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Sample records for based cultivation method

  1. Diversity surveys of soil bacterial community by cultivation-based methods and molecular fingerprinting techniques

    Institute of Scientific and Technical Information of China (English)

    LUO Hai-feng; QI Hong-yan; ZHANG Hong-xun

    2004-01-01

    By combining the cultivation methods with molecular fingerprinting techniques, the diversity surveys of soil bacterial community in 13 areas of China were carried out. The cultivable heterotrophic diversity was investigated by colony morphology on solid LB medium. Genetic diversity was measured as bands on denaturing gradient gel electrophoresis(DGGE) by the extraction and purification of the total soil DNA, and amplification of bacterial 16S rDNA fragments by polymerase chain reaction ( PCR). The Shannon-Wiener indices of diversity (H), richness (S)and evenness( EH ) were employed to estimate the diversity of soil bacterial community. The results showed that there was an obvious diversification existed in soil from the different areas. However, the genetic diversity estimated by PCR-DGGE can provide more comprehensive information on bacterial community than the cultivation-based methods. Therefore, it is suggested to combine the traditional methods with genetic fingerprinting techniques to survey and estimate soil bacterial diversity.

  2. Assessment of Cultivation Method for Energy Beet Based on LCA Method

    Institute of Scientific and Technical Information of China (English)

    Chunfeng; ZHANG; Feng; LIU; Yuangang; ZU; Qingying; MENG; Baoguo; ZHU; Nannan; WANG

    2014-01-01

    In order to establish a supply system for energy resource coupled with the environment,the production technology of sugar beets was explored as a biological energy source. The low-humic andosol as the experimental soil,the panting method was direct planting,and cultivation technique was minimum tillage direct planting method. The control was conventional tillage transplant and no tillage direct planting. The results demonstrated that data revealed that the energy cost of no tillage and a direct planting method was 105 GJ/hm2on average for two years,while that of the conventional tillage method was 112 GJ/hm2per year. The ratio of output to input showed that the direct planting with no tillage was more efficient( 3. 61) than the conventional tillage( 3. 01). Moreover,the emission of CO2into the atmosphere with no tillage and the direct planting was 71% of the conventional tillage planting technique. Therefore,direct planting without tillage reduces the impact on the environment.

  3. Advanced continuous cultivation methods for systems microbiology.

    Science.gov (United States)

    Adamberg, Kaarel; Valgepea, Kaspar; Vilu, Raivo

    2015-09-01

    Increasing the throughput of systems biology-based experimental characterization of in silico-designed strains has great potential for accelerating the development of cell factories. For this, analysis of metabolism in the steady state is essential as only this enables the unequivocal definition of the physiological state of cells, which is needed for the complete description and in silico reconstruction of their phenotypes. In this review, we show that for a systems microbiology approach, high-resolution characterization of metabolism in the steady state--growth space analysis (GSA)--can be achieved by using advanced continuous cultivation methods termed changestats. In changestats, an environmental parameter is continuously changed at a constant rate within one experiment whilst maintaining cells in the physiological steady state similar to chemostats. This increases the resolution and throughput of GSA compared with chemostats, and, moreover, enables following of the dynamics of metabolism and detection of metabolic switch-points and optimal growth conditions. We also describe the concept, challenge and necessary criteria of the systematic analysis of steady-state metabolism. Finally, we propose that such systematic characterization of the steady-state growth space of cells using changestats has value not only for fundamental studies of metabolism, but also for systems biology-based metabolic engineering of cell factories.

  4. Advanced continuous cultivation methods for systems microbiology.

    Science.gov (United States)

    Adamberg, Kaarel; Valgepea, Kaspar; Vilu, Raivo

    2015-09-01

    Increasing the throughput of systems biology-based experimental characterization of in silico-designed strains has great potential for accelerating the development of cell factories. For this, analysis of metabolism in the steady state is essential as only this enables the unequivocal definition of the physiological state of cells, which is needed for the complete description and in silico reconstruction of their phenotypes. In this review, we show that for a systems microbiology approach, high-resolution characterization of metabolism in the steady state--growth space analysis (GSA)--can be achieved by using advanced continuous cultivation methods termed changestats. In changestats, an environmental parameter is continuously changed at a constant rate within one experiment whilst maintaining cells in the physiological steady state similar to chemostats. This increases the resolution and throughput of GSA compared with chemostats, and, moreover, enables following of the dynamics of metabolism and detection of metabolic switch-points and optimal growth conditions. We also describe the concept, challenge and necessary criteria of the systematic analysis of steady-state metabolism. Finally, we propose that such systematic characterization of the steady-state growth space of cells using changestats has value not only for fundamental studies of metabolism, but also for systems biology-based metabolic engineering of cell factories. PMID:26220303

  5. A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures

    Directory of Open Access Journals (Sweden)

    Glumoff Tuomo

    2010-02-01

    Full Text Available Abstract Background Cultivations for recombinant protein production in shake flasks should provide high cell densities, high protein productivity per cell and good protein quality. The methods described in laboratory handbooks often fail to reach these goals due to oxygen depletion, lack of pH control and the necessity to use low induction cell densities. In this article we describe the impact of a novel enzymatically controlled fed-batch cultivation technology on recombinant protein production in Escherichia coli in simple shaken cultures. Results The enzymatic glucose release system together with a well-balanced combination of mineral salts and complex medium additives provided high cell densities, high protein yields and a considerably improved proportion of soluble proteins in harvested cells. The cultivation method consists of three steps: 1 controlled growth by glucose-limited fed-batch to OD600 ~10, 2 addition of growth boosters together with an inducer providing efficient protein synthesis within a 3 to 6 hours period, and 3 a slow growth period (16 to 21 hours during which the recombinant protein is slowly synthesized and folded. Cell densities corresponding to 10 to 15 g l-1 cell dry weight could be achieved with the developed technique. In comparison to standard cultures in LB, Terrific Broth and mineral salt medium, we typically achieved over 10-fold higher volumetric yields of soluble recombinant proteins. Conclusions We have demonstrated that by applying the novel EnBase® Flo cultivation system in shaken cultures high cell densities can be obtained without impairing the productivity per cell. Especially the yield of soluble (correctly folded proteins was significantly improved in comparison to commonly used LB, Terrific Broth or mineral salt media. This improvement is thought to result from a well controlled physiological state during the whole process. The higher volumetric yields enable the use of lower culture volumes and can

  6. Identification of growth phases and influencing factors in cultivations with AGE1.HN cells using set-based methods.

    Directory of Open Access Journals (Sweden)

    Steffen Borchers

    Full Text Available Production of bio-pharmaceuticals in cell culture, such as mammalian cells, is challenging. Mathematical models can provide support to the analysis, optimization, and the operation of production processes. In particular, unstructured models are suited for these purposes, since they can be tailored to particular process conditions. To this end, growth phases and the most relevant factors influencing cell growth and product formation have to be identified. Due to noisy and erroneous experimental data, unknown kinetic parameters, and the large number of combinations of influencing factors, currently there are only limited structured approaches to tackle these issues. We outline a structured set-based approach to identify different growth phases and the factors influencing cell growth and metabolism. To this end, measurement uncertainties are taken explicitly into account to bound the time-dependent specific growth rate based on the observed increase of the cell concentration. Based on the bounds on the specific growth rate, we can identify qualitatively different growth phases and (in-validate hypotheses on the factors influencing cell growth and metabolism. We apply the approach to a mammalian suspension cell line (AGE1.HN. We show that growth in batch culture can be divided into two main growth phases. The initial phase is characterized by exponential growth dynamics, which can be described consistently by a relatively simple unstructured and segregated model. The subsequent phase is characterized by a decrease in the specific growth rate, which, as shown, results from substrate limitation and the pH of the medium. An extended model is provided which describes the observed dynamics of cell growth and main metabolites, and the corresponding kinetic parameters as well as their confidence intervals are estimated. The study is complemented by an uncertainty and outlier analysis. Overall, we demonstrate utility of set-based methods for analyzing cell

  7. Identification of growth phases and influencing factors in cultivations with AGE1.HN cells using set-based methods.

    Science.gov (United States)

    Borchers, Steffen; Freund, Susann; Rath, Alexander; Streif, Stefan; Reichl, Udo; Findeisen, Rolf

    2013-01-01

    Production of bio-pharmaceuticals in cell culture, such as mammalian cells, is challenging. Mathematical models can provide support to the analysis, optimization, and the operation of production processes. In particular, unstructured models are suited for these purposes, since they can be tailored to particular process conditions. To this end, growth phases and the most relevant factors influencing cell growth and product formation have to be identified. Due to noisy and erroneous experimental data, unknown kinetic parameters, and the large number of combinations of influencing factors, currently there are only limited structured approaches to tackle these issues. We outline a structured set-based approach to identify different growth phases and the factors influencing cell growth and metabolism. To this end, measurement uncertainties are taken explicitly into account to bound the time-dependent specific growth rate based on the observed increase of the cell concentration. Based on the bounds on the specific growth rate, we can identify qualitatively different growth phases and (in-)validate hypotheses on the factors influencing cell growth and metabolism. We apply the approach to a mammalian suspension cell line (AGE1.HN). We show that growth in batch culture can be divided into two main growth phases. The initial phase is characterized by exponential growth dynamics, which can be described consistently by a relatively simple unstructured and segregated model. The subsequent phase is characterized by a decrease in the specific growth rate, which, as shown, results from substrate limitation and the pH of the medium. An extended model is provided which describes the observed dynamics of cell growth and main metabolites, and the corresponding kinetic parameters as well as their confidence intervals are estimated. The study is complemented by an uncertainty and outlier analysis. Overall, we demonstrate utility of set-based methods for analyzing cell growth and

  8. An isotope approach based on C-13 pulse-chase labelling vs. the root trenching method to separate heterotrophic and autotrophic respiration in cultivated peatlands

    Energy Technology Data Exchange (ETDEWEB)

    Biasi, C.; Pitkamaki, A. S.; Tavi, N. M.; Koponen, H. T.; Martikainen, P. J. [Univ.of Eastern Finland, Kuopio (Finland). Dept. of Environmental Science], e-mail: christina.biasi@uef.fi

    2012-11-01

    We tested an isotope method based on C-13 pulse-chase labelling for determining the fractional contribution of soil microbial respiration to overall soil respiration in an organic soil (cutaway peatland, eastern Finland), cultivated with the bioenergy crop, reed canary grass. The plants were exposed to CO{sub 2}-13 for five hours and the label was thereafter determined in CO{sub 2} derived from the soil-root system. A two-pool isotope mixing model was used to separate sources of respiration. The isotopic approach showed that a minimum of 50% of the total CO{sub 2} originated from soil-microbial respiration. Even though the method uses undisturbed soil-plant systems, it has limitations concerning the experimental determination of the true isotopic signal of all components contributing to autotrophic respiration. A trenching experiment which was comparatively conducted resulted in a 71% fractional contribution of soil-microbial respiration. This value was likely overestimated. Further studies are needed to evaluate critically the output from these two partitioning approaches. (orig.)

  9. A Study on the Cultivation of College Students' Information Literacy Based on Task-driving Method%基于任务驱动法培养大学生信息素养的研究

    Institute of Scientific and Technical Information of China (English)

    丛荣华

    2011-01-01

    Through analyzing the significance of cultivating college students' information literacy and explaining the relation between information literacy and innovation ability training,this paper puts forward some strategies of cultivating college students' information literacy based on task-driving method.%本文通过对大学生信息素养培养意义的分析以及对信息素养与创新能力培养的关系的说明,详细论述了采用任务驱动法培养大学生信息素养的策略。

  10. Methods for cultivation of luminal parasitic protists of clinical importance.

    Science.gov (United States)

    Clark, C Graham; Diamond, Louis S

    2002-07-01

    Cultivation of luminal protistan parasites has a long history. In this review we discuss the methods and media that are most widely used for the establishment and maintenance of the following organisms in culture: Entamoeba histolytica, Giardia intestinalis, Trichomonas vaginalis, Dientamoeba fragilis, Blastocystis hominis, and Balantidium coli. While cultivation is of limited importance in the diagnostic laboratory, it is essential to most research laboratories, and it is toward the latter that this review is primarily aimed. PMID:12097242

  11. Comparative Study of Rice Morphogenesis wit Different Cultivation Methods

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    [Objective] The aim of this study was to compare the morphological char- acteristics of rice (Oryza sativa L.) with different cultivation methods and investigate the dynamics of organ growth and development characteristics of different rice culti- vars. [Method] Based on continuous field observation and destructive sampling over a growing season, detailed organ morphological data were obtained including leaf length, node number, plant height, tiller number, leaf angle, leaf area and specific leaf weight, to compare organ morphological differences among 4 rice cultivars of Baidao (indica), Jinnanfeng (japonica), 9325 (japonica) and 9915 (japonica) with 3 cultivation methods of field planting in Weigang, pot planting in Weigang, and field planting in Jiangpu. [Result] Maximum leaf length of each node gradually increased at the early growth stage and decreased at the later growth stage, the relationship between maximum leaf length and node position can be described by the equations y=a,,-~ and y=ax+b; node number, growth duration, leaf length and plant height of pot planting rice in Weigang were smaller than that of the other two field planting meth- ods; the relationship between plant height and sunshine duration, plant height and GDD (growing degree days) can be described by the equation y=ax+b, 19.23 ℃.d of GDD (≥10 ≥-d) and 8.12 h of sunshine duration were required to increase 1 cm of plant height; plant height, tiller number, and leaf area of Baidao were higher than that of the other 3 laponica rice cultivars, but the specific leaf weight and leaf angle were smaller. [Conclusion] Comparison of morphological characteristic differ- ences among rice cultivars is an important way to select water-saving and drought- tolerant rice varieties. In this study, the experimental results can be integrated into a rice functional-architectural model to simulate rice organ growth dynamics in a three- dimensional space, thereby providing reference for selecting water

  12. 基于空间模拟退火算法的耕地质量布样及优化方法%Sampling and optimizing methods of cultivated land quality based on spatial simulated annealing algorithm

    Institute of Scientific and Technical Information of China (English)

    杨建宇; 岳彦利; 宋海荣; 汤赛; 叶思菁; 徐凡

    2015-01-01

    耕地质量监测是保障耕地资源的永续利用,实现耕地产能提升、加强耕地资源的管理、保护、合理利用的重要措施,对实现持续粮食安全具有重要意义.该文提出了基于空间模拟退火算法的耕地质量布样优化方法,以空间模拟退火算法为基础生成一组最优样本,构成基础监测网络,在此基础上,通过多期耕地等级成果数据提取属性发生变化的分等因素和对应发生变化的区域,生成潜在变化区,并结合研究区实际情况辅以专家知识和异常监测点,对基础样本点进行增加、删除、替换等优化操作,生成最终监测样点.以北京市大兴区为例,最终确定布设55个监测样点,结果表明,该方法布设的样点在耕地质量预测方面的精度高于传统的随机抽样和分层抽样方法,能有效地预测县域耕地质量并监控耕地质量的变化情况.%M Monitoring points in country area are the foundation to reflect changes of cultivated land quality, which directly affect the result of farmland grading and its accuracy. Through the monitoring network for cultivated land quality in county area, the distribution and changing trend of the cultivated land quality can be reflected. Besides, the quality of non-sampled locations should also be estimated with the data of sampling points. Due to the correlation among spatial samples, the traditional methods such as simple random sampling, stratified sampling and systematic sampling are inefficient to accomplish the task above. Thus, we propose a new spatial sampling and optimizing method based on the spatial simulated annealing (SSA). This paper presents a pre-processing method to determine the number of sampling points, including preprocessing the data of cultivated land quality before sampling, exploring the spatial correlation and spatial distribution pattern of cultivated land quality, and computing the appropriate quantity of sampling points by analyzing the

  13. Sampling method for monitoring classification of cultivated land in county area based on Kriging estimation error%基于Kriging估计误差的县域耕地等级监测布样方法

    Institute of Scientific and Technical Information of China (English)

    杨建宇; 汤赛; 郧文聚; 张超; 朱德海; 陈彦清

    2013-01-01

    China, an agricultural country, has a large population but not enough cultivated land. Until 2011, the cultivated land per capita was 1.38 mu (0.09 ha), only 40% of the world average, and it is getting worse with industrialization and urbanization. The next task for the Ministry of Land and Resources:Dynamic monitoring of cultivated land classification in which a number of counties will be sampled; in each county, a sample-based monitoring network would be established that reflects the distribution and its tendency of cultivated land classification in county area and estimates of non-sampled locations. Due to the correlation among samples, traditional methods such as simple random sampling, stratified sampling, and systematic sampling are insufficient to achieve the goal. Therefore, in this paper we introduced a spatial sampling method based on the Kriging estimation error. For our case, natural classifications of cultivated land identified from the last Land Resource Survey and Cultivated Land Evaluation are regarded as the true value and classifications of non-sampled cultivated lands would be predicted by interpolating the sample data. Finally, RMSE (root-mean-square error) of Kriging interpolation is redefined to measure the performance of the network. To be specific, five steps are needed for the monitoring network. First, the optimal sample size is determined by analyzing the variation trend between the number and the accuracy of samples. Then, set up the basic monitoring network using square grids. The suitable grid size can be chosen by comparing the grid sizes and the corresponding RMSEs from the Kriging interpolation of the samples data. Because some centers of grids do not overlap the area of cultivated land, the third step is to add some points near the centers of grids to create the global monitoring network. These points are selected from centroids of cultivated land spots which are closest to the centers and inside the searching circles around the

  14. Component analysis of cultivated ginseng, cultivated wild ginseng, and natural wild ginseng by structural parts using HPLC method

    OpenAIRE

    Young-Ju, Han; Ki-Rok, Kwon; Bae-Chun,Cha; Oh-Man,Kwon

    2007-01-01

    Objectives : The aim of this experiments is to provide an objective differentiation of ginseng, Korean and Chinese cultivated wild ginseng, and natural wild ginseng through components analysis of different parts of ginseng. Methods : Comparative analyses of ginsenoside-, ginsenoside-, and ginsenosides and from the root, stem, and leaves of ginseng, Korean and Chinese cultivated wild ginseng, and natural wild ginseng were conducted using HPLC. Results : 1. For content comparison of lea...

  15. A Moral Method? Thoughts on Cultivating Empathy through Method Acting.

    Science.gov (United States)

    Verducci, Susan

    2000-01-01

    Using Nel Noddings ethic of Care, contends that this model of empathy has elements in common with the type of empathy method actors employ. Discusses the agreements between Caring's empathy and dramatic empathy focusing on three skills method actors use and the three differences that possess moral implications. (CMK)

  16. Dynamics of flowering of artichoke globe (Cynara scolymus L.) plants in depending on cultivation method

    OpenAIRE

    Andrzej Sałata

    2012-01-01

    The study was carried out in the years 2001-2002 in the Felin Research Center in Lublin. The differences in growth dynamics and morphology of flowering shoots were investigated with regards to a method of cultivation of artichoke. In the year 2001 flowering shoots and flowers occurred the earliest in the year 2001 on artichoke plants cultivated from crowns. In the year 2002 plants cultivated from crowns and transplants produced flowering shoots in the same time. Artichoke plants cultivated fr...

  17. Component analysis of cultivated ginseng, cultivated wild ginseng, and natural wild ginseng by structural parts using HPLC method

    Directory of Open Access Journals (Sweden)

    Young-Ju,Han

    2007-02-01

    Full Text Available Objectives : The aim of this experiments is to provide an objective differentiation of ginseng, Korean and Chinese cultivated wild ginseng, and natural wild ginseng through components analysis of different parts of ginseng. Methods : Comparative analyses of ginsenoside-, ginsenoside-, and ginsenosides and from the root, stem, and leaves of ginseng, Korean and Chinese cultivated wild ginseng, and natural wild ginseng were conducted using HPLC. Results : 1. For content comparison of leaves, ginseng showed highest content of ginsenoside than other samples. Natural wild ginseng showed relatively high content of ginsenosides and than other samples. 2. For content comparison of the stem, ginseng and 10 years old Chinese cultivated wild ginseng didn't contain ginsenoside . Natural wild ginseng showed higher content of ginsenosides and than other samples. 3. For content comparison of the root, ginsenoside was found only in 5 and 10 years old Korean cultivated wild ginseng. 4. Distribution of contents by the parts of ginseng was similar in ginseng and Chinese cultivated wild ginseng. Conclusions : Above experiment data can be an important indicator for the identification of ginseng, Korean and Chinese cultivated wild ginseng, and natural wild ginseng.

  18. LTCC based bioreactors for cell cultivation

    Science.gov (United States)

    Bartsch, H.; Welker, T.; Welker, K.; Witte, H.; Müller, J.

    2016-01-01

    LTCC multilayers offer a wide range of structural options and flexibility of connections not available in standard thin film technology. Therefore they are considered as material base for cell culture reactors. The integration of microfluidic handling systems and features for optical and electrical capturing of indicators for cell culture growth offers the platform for an open system concept. The present paper assesses different approaches for the creation of microfluidic channels in LTCC multilayers. Basic functions required for the fluid management in bioreactors include temperature and flow control. Both features can be realized with integrated heaters and temperature sensors in LTCC multilayers. Technological conditions for the integration of such elements into bioreactors are analysed. The temperature regulation for the system makes use of NTC thermistor sensors which serve as real value input for the control of the heater. It allows the adjustment of the fluid temperature with an accuracy of 0.2 K. The tempered fluid flows through the cell culture chamber. Inside of this chamber a thick film electrode array monitors the impedance as an indicator for the growth process of 3-dimensional cell cultures. At the system output a flow sensor is arranged to monitor the continual flow. For this purpose a calorimetric sensor is implemented, and its crucial design parameters are discussed. Thus, the work presented gives an overview on the current status of LTCC based fluid management for cell culture reactors, which provides a promising base for the automation of cell culture processes.

  19. Integrated Indicators-based Gradation of Cultivated Land Quality in Mountainous Region of Southwestern China

    Institute of Scientific and Technical Information of China (English)

    SHAO Jing'an; WEI Chaofu; XIE Deti; ZHOU Yan

    2006-01-01

    The gradation of cultivated land is to assess the suitability of cultivated land for agricultural production in terms of natural and economic properties of land. It can be used to evaluate sustainability of land use and soil management practices. Formal and informal surveys, Delphi and comprehensive index method are adopted to identify appropriate integrated indicators, and measure the gradation of cultivated land quality. The determination of integrated indicators presents three basic features of cultivated land quality: stable plant productivity, social acceptability and maintenance of environmental quality.Pronounced concentration-dissipation law of total function score value occurs in paddy field units, with three ranges, I.e., 79-68.5, 68.5~59 and 59-51. Total function score value in dryland units mainly ranges from 40 to 70, but slight concentration-dissipation law of each unit is still observed, with four ranges, I.e., 79~68, 68~51, 51~37 and 37~15. Paddy field quality is divided into three gradations, and the scales are 18 220.9 ha, 5410.6 ha and 2890.9 ha. Dryland quality is classified into four gradations, and the scales are 1548.6 ha, 8153.8 ha, 3316.3 ha and 685.2 ha. The total function score value of every gradation unit is conformed to the results of farmers' assessment. Research results meet with the needs of accurate degree of the gradation of cultivated land quality. Integrated indicators-based gradation of cultivated land assessment accords with the characteristics of land resources and human preference in the mountains of southwestern China. This study will be useful to promote the monitoring of cultivated land quality, and to supply fine ground and knowledge for establishing appropriate cultivated land management practices in Southwestern China.

  20. Component Analysis of Cultivated Ginseng, Red Ginseng, Cultivated Wild Ginseng, and Red Wild Ginseng Using HPLC Method

    Directory of Open Access Journals (Sweden)

    Jang Ho, Lee

    2008-06-01

    Full Text Available Objectives : The aim of this experiment is to provide an differentiation of ginseng, red ginseng, cultivated wild ginseng(CWG, and red wild ginseng(RWG through component analysis using HPLC(High Performance Liquid Chromatography, hereafter HPLC. Methods : Comparative analyses of ginsenoside Rg3, ginsenoside Rh2, and ginsenosides Rb1 and Rg1 of various ginsengs were conducted using HPLC. Results : 1. CWG was relatively heat-resistant and showed slow change in color during the process of steaming and drying, compared to cultivated ginseng. 2. Ginsenoside Rg3 was not detected in cultivated ginseng and CWG, whereas it was high in red ginseng and RWG. Ginsenoside Rg3 was more generated in red ginseng than in RWG. 3. Ginsenoside Rh2 appreared during steaming and drying of cultivated ginseng, whereas it was more increased during steaming and drying of CWG. 4. Ginsenoside Rg1 content was more increased during steaming and drying of cultivated ginseng, whereas it was more decreased during steaming and drying of CWG. 5. Ginsenoside Rb1 content was increased about 500% during steaming and drying of cultivated ginseng, whereas it was increased about 30% during steaming and drying of CWG, indicating that ginsenoside Rb1 was more generated in red ginseng than in RWG. 6. Ginsenoside Rg3 content was higher, whereas ginsenoside Rg1 content was lower in 11th RWG than in 9th RWG, indicating that ginsenoside Rg3 content was increased and Rg1 content was decreased as steaming and drying continued to proceed. Ginsenoside Rh2 and Rb1 contents began to be increased, followed by decreased after 9th steaming and drying process. Conclusions : Above experiment data can be an important indicator for the identification of ginseng, red ginseng, CWG, and RWG. And the following studies will be need for making good product using CWG.

  1. Simple and efficient method for isolation and cultivation of endoscopically obtained human colonocytes

    DEFF Research Database (Denmark)

    Seidelin, Jakob B; Horn, Thomas; Nielsen, Ole H

    2003-01-01

    Few comparative and validated reports exist on the isolation and growth of colonoscopically obtained colonic epithelium. The aim of this study was to develop and validate a simple method for the cultivation of colonoscopically obtained colonocytes. Forty patients, who underwent routine colonoscop....... A simple method is presented, which makes cultivation of colonocytes obtained at endoscopy possible for up to 72 h....

  2. 基于改进农业生态区划法的耕地综合量能指数模型构建%Comprehensive index model building of cultivated land yield and productivity based on improved agro-ecological zoning method

    Institute of Scientific and Technical Information of China (English)

    陈丽; 郝晋珉; 艾东; 朱传民; 周宁; 洪舒蔓

    2014-01-01

    研究粮食产量与耕地产能间的关系以及区域差异,对于挖掘区域增产潜力,提高耕地资源利用效率,实现区域均衡增产,保障粮食安全战略具有重要意义。该文在农业生态区划法估算土地生产潜力基础上,提出基于耕地质量新内涵的耕地质量修正的耕地产能核算方法。通过探讨耕地综合产能的概念体系,构建了反映粮食产量与耕地产能关系的耕地综合量能指数模型,模型由耕地综合量能指数、耕地质量产能指数和耕地数量产能指数构成。并以曲周县为例,对该模型进行了实证研究,曲周县耕地综合量能指数为0.16,耕地质量产能指数为0.31,耕地数量产能指数为0.99,耕地总体产出效率不高,粮食增产的潜力较大,耕地粮食生产平均开发程度也不高,但数量增加潜力较小。%Study on the relationships between grain production and cultivated land productivity and its regional difference is of great significance for food security. Besides, it is also important for finding regional potential yields of grain, improving the utilization efficiency of cultivated land resources and achieving the regional increase of grain yield. In this paper, on the basis of agro-ecological zoning (AEZ) model, a new method was proposed to calculate the cultivated land productivity under cultivated land quality modification. Furthermore, the modification of cultivated land quality was based on a new concept that the quality of cultivated land was not only affected by natural factors but also by artificial factors such as irrigation canals, greenhouse, nursery factory, mechanical facilities and its supporting service station. By discussing the conceptual system of cultivated land integrated productivity which took the potential quantity of cultivated land and the quality index of cultivated land into the consideration, we proposed that comprehensive productivity of cultivated land

  3. Component Analysis of Cultivated Ginseng, Red Ginseng, Cultivated Wild Ginseng, and Red Wild Ginseng Using HPLC Method

    OpenAIRE

    Jang Ho, Lee; Ki Rok, Kwon; Bae Chun, Cha

    2008-01-01

    Objectives : The aim of this experiment is to provide an differentiation of ginseng, red ginseng, cultivated wild ginseng(CWG), and red wild ginseng(RWG) through component analysis using HPLC(High Performance Liquid Chromatography, hereafter HPLC). Methods : Comparative analyses of ginsenoside Rg3, ginsenoside Rh2, and ginsenosides Rb1 and Rg1 of various ginsengs were conducted using HPLC. Results : 1. CWG was relatively heat-resistant and showed slow change in color during the process ...

  4. Experimental Study on Granular Sludge Cultivation Based on IC Reactor

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    [Objective]We aimed to discuss the optimal conditions of sludge granulation on the basis of IC reactor.[Method]By using the formulated glucose wastewater,we studied the rapid cultivation of granular sludge as well as its influencing factors,so as to discuss the optimal conditions of sludge granulation.[Result]Through the static culture outside of IC reactor and dynamic culture in IC reactor,granular sludge appeared within a training cycle,with particle size of 1.0-1.5 mm.In IC reactor,when COD concentration...

  5. Comparison of the Anaerocult A and the oil blocking methods for the in vitro cultivation of Entamoeba histolytica.

    Science.gov (United States)

    Mohd Shah, Nurul Azira; Wan Abdul Wahab, Wan Nor Amilah; Mohd Nawi, Siti Farah Alwani; Mohd-Zain, Zaini; Latif, Baha; Suhaimi, Rosliza

    2015-12-01

    Entamoeba histolytica, the causative agent for human amoebiasis, is among the most deadly parasites, accounting for the second highest mortality rate among parasitic diseases. Because this parasite dwells in low oxygen tension, for its cultivation, microaerophilic conditions are required to mimick the human gut environment. Several methods developed for optimal growth environment are commercially available and some are conventionally modified in-house which include the Anaerocult A and oil blocking preparation methods. This study was undertaken to compare the reliability of the Anaerocult A and the oil blocking methods in generating anaerobic environment for cultivation of E. histolytica. The trophozoites of E. histolytica HM1: IMSS strains were axenically cultivated in TYI-S-33 medium in culture incubated anaerobically by using Anaerocult A (Merck) and mineral oil blocking method. The outcomes of both methods were determined by the minimum inhibitory concentration (MIC) of metronidazole against E. histolytica by giving a score to the growth pattern of the trophozoites. The reliability of both methods was assessed based on susceptibility testing of E. histolytica to metronidazole. The MIC obtained by both anaerobic condition methods was 6.25 ug/ ml, thus showing that oil-blocking method is comparable to the Anaerocult A method and therefore, considered as a reliable method for generating an anaerobic environment for the cultivation of E. histolytica.

  6. To Cultivate Outstanding Software Engineer Based on Project-based Learning

    Institute of Scientific and Technical Information of China (English)

    LI Dong; MA Peiqun; LIU Shu

    2012-01-01

    This thesis sketches the connotation of project-based learning and introduces the basis on which project-based learning is practiced and applied in school of software as well as the plan of further practicing project-based learning. At the same time, this thesis also discusses application of project-based learning in "education and cultivation plan of excellent engineer".

  7. Application of gamma-ray transmission method for study the properties of cultivated soil

    International Nuclear Information System (INIS)

    Highlights: ► Gamma-ray transmission method and attenuation coefficients. ► Physical properties of cultivated soil. ► Soil–water systems management. - Abstract: The objective of the proposed work was to apply gamma-ray transmission method for studying the properties of cultivated soil. In this study, mass attenuation coefficients, bulk density, moisture content, porosity, and field capacity are determined. Five soil samples are collected from different agriculture zones in Egypt. A high-resolution gamma-ray spectrometry based on HPGe (high purity germanium) detector was employed to measure attenuation of strongly collimated monoenergetic gamma beam through soil samples. The radioactive sources used in the experiment were 241Am, 133Ba, 137Cs and 60Co. The mass attenuation coefficients of soil samples were calculated from the transmission measurements. WinXCom program was used to calculate mass attenuation coefficients of soil samples. There is a satisfactory agreement between experimental and theoretical values. It was observed that gamma-ray transmission method is an efficient tool for studying soil parameters for agriculture purposes.

  8. 基于双边界二分式CVM的耕地社会价值评价——以重庆市为例%Appraisal of Cultivated Land Social Vaiue Based on Double-Bounded Contingent Valuation Method

    Institute of Scientific and Technical Information of China (English)

    唐建; 易乐庆; 彭珏

    2011-01-01

    以重庆市为例,采用双边界二分式CVM的logistic模型,从城镇居民支付意愿和农民受偿意愿2个角度考察耕地的社会价值.研究发现,WTP和WTA法下,耕地的社会价值巨大,且呈增长态势;城镇总体支付意愿和农民总体受偿意愿存在较大差距,目前仍需政府采取农业补贴等形式解决耕地外部性问题.%Taking the case of Chongqing, logistic model of double-bounded CVM is adopted, the social value of cultivated land are investigated from the angle of urban residents' willingness to pay and peasants' willingness to accept. The results show that there is great social value of cultivated land under WTP and WTA methods, and being a rising trend; there is a wide gap between urban WTP and rural WTA, and it still need the government to take agricultural subsidies to solve land externalities at present.

  9. Potential of Homestay Tourism Based on Seaweed Cultivation from the Views of Seaweed Cultivators in District of Semporna Sabah, East Malaysia

    Directory of Open Access Journals (Sweden)

    Hussin Rosazman

    2014-01-01

    Full Text Available Community participation in tourism development especially among fisherman and farmers has begun to given serious attention by the government whereby the communities are given opportunities to engage in tourism development programmes in order to enhance their quality of life. In order to encourage local community participation in tourism development in rural areas, participants’ perceptions regarding tourism activities are important aspect to be sought. Good or bad perceptions from the community towards tourism development are important because it can determine the success of the programme. Firstly, this paper aims to explore the views or perceptions of seaweed cultivators towards homestay tourism which is based on seaweed cultivation in the District of Semporna, Sabah. Qualitative and quantitative research approaches have been applied in this study, such as the usage of the face to face interviews survey using survey questionnaires and field observation as primary methods. The findings show that the majority of the respondents have a positive perception of homestay tourism based on seaweed cultivation, such as the acceptance of visits by the tourists to their working place. Seaweed cultivators agreed that this tourism activity bring additional income to them. Activities that can become tourist attractions include tying seaweed seeds on a casino table. Moreover, the tourists have an opportunity to take a boat to see the seaweed farm, and take pictures of seaweed activities and so on. These findings also revealed that the majority of the respondents assumed that the visits of the tourists would motivate them to carry out the activity with more enthusiasm. This shows that seaweed cultivation could become a new tourism product which has great potential to develop in the district of Semporna, Sabah.

  10. Appraisal of the Ecological Value of Cultivated Land Based on Double-Bounded Contingent Valuation Methods%基于双边界二分式CVM法的耕地生态价值评价——以重庆市为例

    Institute of Scientific and Technical Information of China (English)

    唐建; 沈田华; 彭珏

    2013-01-01

    There are externalities in the use of cultivated land, but there is no rational compensation for positive externality, and no effective restraint and containment for negative externality in China. Under the present system, fanners make use of their cultivated land for economic interests and ignore social and ecological opportunity costs. This pattern will lead to a reduction in cultivated land, quality degradation and environment pollution, and also seriously threaten food safety and sustainable agriculture. The ecological value of cultivated land should be evaluated objectively in order to reveal the extent of cultivated land ecological externalities. Research into the evaluation of the ecological value of cultivated land remains in the primary phase as there are many different methods with various results, most of which focus on urban WTP and ignore rural WTA. If WTA is ignored, it is difficult to build a rational compensation policy and incentive mechanisms for the ecological value of cultivated land. In order to solve this problem, views of urban WTP and rural WTA are taken into consideration. This paper uses Chongqing city as a focal city and applies logistic models of double-bounded contingent valuation methods to evaluate the ecological value of cultivated land. The results indicate that based on a 5% discount rate, the total ecological value of cultivated land in Chongqing is 87.6 billion CNY with WTP views and 213.2 billion CNY with WTA views. There is important ecological value under WTP and WTA method. There is a wide gap between urban WTP and rural WTA and therefore the government need to adopt measures to solve the ecological externalities of cultivated land, such as through agricultural subsidies.%评价耕地生态价值有利于了解耕地生态外部性大小,为政府制订合理的耕地补偿政策提供依据,实现耕地资源的可持续利用.本文以重庆市为例,采用双边界二分式CVM法的Logistic模型,从城镇居民的支付

  11. Curriculum-based library instruction from cultivating faculty relationships to assessment

    CERN Document Server

    Blevins, Amy

    2014-01-01

    Curriculum-Based Library Instruction: From Cultivating Faculty Relationships to Assessment highlights the movement beyond one-shot instruction sessions, specifically focusing on situations where academic librarians have developed curriculum based sessions and/or become involved in curriculum committees.

  12. Quantification of encapsulated bioburden in spacecraft polymer materials by cultivation-dependent and molecular methods.

    Directory of Open Access Journals (Sweden)

    Anja Bauermeister

    Full Text Available Bioburden encapsulated in spacecraft polymers (such as adhesives and coatings poses a potential risk to jeopardize scientific exploration of other celestial bodies. This is particularly critical for spacecraft components intended for hard landing. So far, it remained unclear if polymers are indeed a source of microbial contamination. In addition, data with respect to survival of microbes during the embedding/polymerization process are sparse. In this study we developed testing strategies to quantitatively examine encapsulated bioburden in five different polymers used frequently and in large quantities on spaceflight hardware. As quantitative extraction of the bioburden from polymerized (solid materials did not prove feasible, contaminants were extracted from uncured precursors. Cultivation-based analyses revealed <0.1-2.5 colony forming units (cfu per cm3 polymer, whereas quantitative PCR-based detection of contaminants indicated considerably higher values, despite low DNA extraction efficiency. Results obtained from this approach reflect the most conservative proxy for encapsulated bioburden, as they give the maximum bioburden of the polymers irrespective of any additional physical and chemical stress occurring during polymerization. To address the latter issue, we deployed an embedding model to elucidate and monitor the physiological status of embedded Bacillus safensis spores in a cured polymer. Staining approaches using AlexaFluor succinimidyl ester 488 (AF488, propidium monoazide (PMA, CTC (5-cyano-2,3-diotolyl tetrazolium chloride demonstrated that embedded spores retained integrity, germination and cultivation ability even after polymerization of the adhesive Scotch-Weld 2216 B/A. Using the methods presented here, we were able to estimate the worst case contribution of encapsulated bioburden in different polymers to the bioburden of spacecraft. We demonstrated that spores were not affected by polymerization processes. Besides Planetary

  13. Strategies for Cultivating New Peasants Based on Modern Agricultural Development

    Institute of Scientific and Technical Information of China (English)

    Fanxing; MENG; Jianguo; ZHOU

    2013-01-01

    Development of modern agriculture has higher and higher requirements on quality of peasants.This study,on the basis of connotations of modern agriculture,current situation and problems of Chinese peasants,and contradictions between modern agricultural development and peasants’ quality,specifies requirements of modern agriculture on new peasants,proposes corresponding suggestions to promote the cultivation of new Chinese peasants.

  14. 基于农用地分等修正的土地整治项目对耕地质量的影响评价%Evaluation on influence of land consolidation project on cultivated land quality based on agricultural land classification correction method

    Institute of Scientific and Technical Information of China (English)

    匡丽花; 叶英聪; 赵小敏; 郭熙; 谢文

    2016-01-01

    natural quality would be affected by the land consolidation project. In this study, the initial grading factors could be divided into two categories, the first were the stable factors, the attribute remained the same after the land consolidation project, and the second were the changing factors, the attribute changed after the land consolidation project. For these stable factors, in this paper, we took the original data directly. For these changing factors, we would collect the data after the land consolidation project through field investigation and indoor analysis. The weights and scoring rules of the natural quality grading factors stayed the same. Then we recalculated the natural quality index again. The canonical correlation analysis method was used to determine the land use correction factors from the influence factors of land consolidation project on the cultivated land production conditions. Then, we determined the weights of the factors on the base of Delphi method and analytic hierarchy process (AHP). The ratio of the scores before and after land consolidation was the land use correction coefficient and modified the original land use coefficient so that the revised land use index could be derived according to the original evaluation process. In the paper, we developed the cultivated land quality natural grade and the land use grade according to Agricultural Land Classification for Jiangxi Province. Then we compared and analyzed the changing situation of cultivated land quality grade before and after land consolidation. At last, in this paper, we used the multiple regression analysis methods to determine correction factors which had significant influence on the cultivated land quality. The land use correction factors were plots regularity, road network density, effective irrigation area index, distribution of the drought and flood. The results showed that the average natural quality grade were raised from 9.06 to 8.81 after the land consolidation, the general

  15. Collection and cultivation methods of Acartia tonsa for toxicity testing

    Energy Technology Data Exchange (ETDEWEB)

    Hood, C.A. [Baker Hughes INTEQ, Houston, TX (United States); Mayo, R.R. [ENSR Environmental Toxicology Lab., Houston, TX (United States)

    1995-12-31

    Acartia tonsa were located and collected from Galveston Bay, Texas in June 1995, using plankton nets and transported to the laboratory for culture. After literature searching and laboratory experimentation. A simple reliable method was designed to culture A. tonsa. This method requires a minimum of glassware and supplies. Adult A. tonsa are placed in one gallon bell jars filled with natural seawater. The jars are then maintained in a water bath at a constant temperature. Water changes are conducted twice weekly and organisms are fed daily with a mixture of algae, Skeletonema costatum, isocrysis galbana, and Thalassiosira sp. Gravid females are then isolated in generators for 24 hours to obtain known age neonates. The neonates are maintained up to a specific age and then are used in toxicity tests such as the ``Determination of the Acute Lethal Toxicity to Marine Copepods,`` required in the United Kingdom for all chemicals used for offshore drilling fluid applications.

  16. Simple and efficient method for isolation and cultivation of endoscopically obtained human colonocytes.

    Science.gov (United States)

    Seidelin, Jakob B; Horn, Thomas; Nielsen, Ole H

    2003-12-01

    Few comparative and validated reports exist on the isolation and growth of colonoscopically obtained colonic epithelium. The aim of this study was to develop and validate a simple method for the cultivation of colonoscopically obtained colonocytes. Forty patients, who underwent routine colonoscopy and where the diagnosis of irritable bowel syndrome was later reached, were included. Seven colon biopsies were taken and incubated at varying time periods of 10-120 min and temperatures of 4-37 degrees C in a chelating buffer. The epithelium was then harvested and cultivated under three different conditions: 1) on a collagen coating, 2) embedded in a collagen gel, or 3) embedded in a gel put on a porous well insert. The effect of conditioned medium (CM), insulin, transferrin, selenium, and the oxygen content was assessed. Viability was tested by the metabolic dimethylthiazol-diphenyl-tetrazolium bromide assay, by flowcytometry, by phase contrast microscopy, and by transmission electron microscopy. Incubation at 21 degrees C for 75 min gave an optimal yield of 3 x 10(6) (2.0-3.8 x 10(6)) viable epithelial cells in intact crypts per seven biopsies. Embedding of crypts in a collagen gel put on a porous membrane was superior to the other methods applied [P coat-cultivated cells. CM had similar viability supporting effects to FCS. Other supplements had no effects. A simple method is presented, which makes cultivation of colonocytes obtained at endoscopy possible for up to 72 h.

  17. Construction of the Classification and Grading Index System of Cultivated Land Based on the Viewpoint of Sustainable Development

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    In order to objectively and reasonably evaluate the actual and potential value of cultivated land, both social and ecological values are introduced into the classification and grading index system of cultivated land based on the viewpoint of sustainable development, after considering the natural and economic values of cultivated land. Index system construction of the sustainable utilization of cultivated land should follow the principles of economic viability, social acceptability, and ecological protection. Classification of cultivated land should take into account the soil fertility of cultivated land. Then, grading of cultivated land is carried out from the practical productivity (or potential productivity) of cultivated land. According to the existing classification index system of cultivated land, the soil, natural and environmental factors in plains, mountains and hills are mainly modified in the classification index system of cultivated land. And index systems for the cultivated land classification in plains, mountains and hills are set up. The grading index system of cultivated land is established based on the economic viability (economic value), social acceptability (social value) and protection of cultivated land (ecological value). Quantitative expression of cultivated land grading index is also carried out.

  18. Validation of a novel sequential cultivation method for the production of enzymatic cocktails from Trichoderma strains.

    Science.gov (United States)

    Florencio, C; Cunha, F M; Badino, A C; Farinas, C S

    2015-02-01

    The development of new cost-effective bioprocesses for the production of cellulolytic enzymes is needed in order to ensure that the conversion of biomass becomes economically viable. The aim of this study was to determine whether a novel sequential solid-state and submerged fermentation method (SF) could be validated for different strains of the Trichoderma genus. Cultivation of the Trichoderma reesei Rut-C30 reference strain under SF using sugarcane bagasse as substrate was shown to be favorable for endoglucanase (EGase) production, resulting in up to 4.2-fold improvement compared with conventional submerged fermentation. Characterization of the enzymes in terms of the optimum pH and temperature for EGase activity and comparison of the hydrolysis profiles obtained using a synthetic substrate did not reveal any qualitative differences among the different cultivation conditions investigated. However, the thermostability of the EGase was influenced by the type of carbon source and cultivation system. All three strains of Trichoderma tested (T. reesei Rut-C30, Trichoderma harzianum, and Trichoderma sp INPA 666) achieved higher enzymatic productivity when cultivated under SF, hence validating the proposed SF method for use with different Trichoderma strains. The results suggest that this bioprocess configuration is a very promising development for the cellulosic biofuels industry.

  19. Cultivating Life Skills at a Project-Based Charter School

    Science.gov (United States)

    Wurdinger, Scott; Enloe, Walter

    2011-01-01

    Surveys that focused on academic and life skill development were collected from alumni who attended Avalon Charter School in St Paul, Minnesota. Avalon is a small public charter school that uses project-based learning as their primary teaching method. Forty-two alumni responded to the online survey. Students ranked life skills such as creativity,…

  20. Research Progress on Cultivation Methods of Microalgae%微藻培养方法研究进展

    Institute of Scientific and Technical Information of China (English)

    尚常花; 朱顺妮; 王忠铭; 袁振宏; 谢君

    2016-01-01

    微藻是一种有前景的生物柴油原料。微藻培养是微藻生物柴油生产过程的重要环节。本文就微藻培养方法的研究进展进行了阐述。对自养、异养及兼养三种培养方法进行了比较,并对微藻培养提出了建议。%Microalgae is a promising feedstock for biodiesel production. Cultivation of microalgae is a key step in the process of producing biodiesel using microalgae as raw material. Research progress on cultivation methods of microalgae is discussed. Phototrophic cultivation, heterotrophic cultivation and mixotrophic cultivation are compared. Suggestions about cultivation of microalgae are proposed in this article.

  1. Effect of planting methods and cyanobacterial inoculants on yield, water productivity and economics of rice cultivation

    Directory of Open Access Journals (Sweden)

    Amit A. Shahane

    2015-09-01

    Full Text Available The impact of two crop planting methods and of the application of cyanobacterial inoculants on plant growth, yield, water productivity and economics of rice cultivation was evaluated with the help of a split plot designed experiment during the rainy season of 2011 in New Delhi, India. Conventional transplanting and system of rice intensification (SRI were tested as two different planting methods and seven treatments that considered cyanobacterial inoculants and compost were applied with three repetitions each. Results revealed no significant differences in plant performance and crop yield between both planting methods. However, the application of biofilm based BGA bio-fertiliser + 2/3 N had an overall positive impact on both, plant performance (plant height, number of tillers and crop yield (number and weight of panicles as well as on grain and straw yield. Higher net return and a higher benefit-cost ratio were observed in rice fields under SRI planting method, whereas the application of BGA + PGPR + 2/3 N resulted in highest values. Total water productivity and irrigation water productivity was significantly higher under SRI practices (5.95 and 3.67 kg ha^(-1 mm^(-1 compared to practices of conventional transplanting (3.36 and 2.44, meaning that using SRI method, water saving of about 34 % could be achieved and significantly less water was required to produce one kg of rice. This study could show that a combination of plant growth promoting rhizobacteria (PGPR in conjunction with BGA and 2/3 dose of mineral N fertiliser can support crop growth performance, crop yields and reduces overall production cost, wherefore this practices should be used in the integrated nutrient management of rice fields in India.

  2. Crop based climate regimes for energy saving in greenhouse cultivation

    Energy Technology Data Exchange (ETDEWEB)

    Koerner, O.

    2003-06-16

    Sustainability is one of the major aims in greenhouse horticulture. According to agreements between the Dutch grower association and the government, energy consumption and the use of chemical biocides have to be reduced. More advanced greenhouse technique is being developed to reach the target to decrease the energy efficiency-index by 65% between 1980 and 2010. However, this could also be achieved with existing technology by using more advanced climate regimes. The present thesis aimed at that, through designing and analysing climate regimes while employing existing climate control possibilities. Theoretical temperature and humidity regimes were designed to decrease energy consumption and a photosynthesis maximisation procedure was implemented to maximise growth. The basis for a crop gross photosynthesis model for control purposes was created. Crop photosynthesis models were evaluated at conditions expected to occur with more sustainable climate regimes. It was shown with experimental evidence that theoretical assumptions on the temperature - CO2 effects in a crop that are based on theoretically models scaling up leaf photosynthesis to the crop level are valid and that simplified existing models could be applied up to 28C. With higher temperatures new designs are needed and this can probably be achieved with an improved stomata-resistance model. The well known temperature integration principle was modified with two nested time-frames (24-hour and six days) and a temperature dose-response function. In a year round tomato cultivation, energy consumption was predicted to decrease with up to 9 % compared to regular temperature integration. The potential for energy saving with temperature integration is limited by humidity control when as usual fixed set points are maintained, because it counteracts temperature integration. Vents open at lower temperatures and heating is switched on at higher temperatures than required for optimal effects of temperature integration. A

  3. Estimation of the sugar cane cultivated area from LANDSAT images using the two phase sampling method

    Science.gov (United States)

    Parada, N. D. J. (Principal Investigator); Cappelletti, C. A.; Mendonca, F. J.; Lee, D. C. L.; Shimabukuro, Y. E.

    1982-01-01

    A two phase sampling method and the optimal sampling segment dimensions for the estimation of sugar cane cultivated area were developed. This technique employs visual interpretations of LANDSAT images and panchromatic aerial photographs considered as the ground truth. The estimates, as a mean value of 100 simulated samples, represent 99.3% of the true value with a CV of approximately 1%; the relative efficiency of the two phase design was 157% when compared with a one phase aerial photographs sample.

  4. Genetic Diversity Based on Allozyme Alleles of Chinese Cultivated Rice

    Institute of Scientific and Technical Information of China (English)

    TANG Sheng-xiang; WEI Xing-hua; JIANG Yun-zhu; D S Brar; G S Khush

    2007-01-01

    Genetic diversity was analyzed with 6 632 core rice cultivars selected from 60 282 Chinese rice accessions on the basis of 12 allozyme loci, Pgil, Pgi2, Ampl, Amp2, Amp3, Amp4, Sdh1, Adh1, Est1, Est2, Est5 and Est9, by starch gel electrophoresis. Among the materials examined, 52 alleles at 12 polymorphic loci were identified, which occupied 96.3% of 54 alleles found in cultivated germplasm of O.sativa L. The number of alleles per locus ranged from 2 to 7 with an average of 4.33. The gene diversity (He) each locus varied considerably from 0.017 for Amp4 to 0.583 for Est2 with an average gene diversity (Ht) 0.271, and Shannon-Wiener index from 0.055 to 0.946 with an average of 0.468. The degree of polymorphism (DP) was in a range from 0.9 to 46.9% with an average of 21.4%. It was found that the genetic diversity in japonica (Keng) subspecies was lower in terms of allele's number, Ht and S-W index, being 91.8, 66.2 and 75.7% of indica (Hsien) one, respectively. Significant genetic differentiation between indica and japonica rice has been appeared in the loci Pgil, Amp2, Pgi2, and Est2, with higher average coefficient of genetic differentiation (Gst) 0.635, 0.626, 0.322 and 0.282, respectively. Except less allele number per locus (3.33) for modern cultivars, being 76.9% of landraces, the Ht and S-W index showed in similar between the modern cultivars and the landraces detected. In terms of allozyme, the rice cultivars in the Southwest Plateau and Central China have richer genetic diversity. The present study reveals again that Chinese cultivated rice germplasm has rich genetic diversity, showed by the allozyme allele variation.

  5. Diversity and habitat preferences of cultivated and uncultivated aerobic methanotrophic bacteria evaluated based on pmoA as molecular marker

    Directory of Open Access Journals (Sweden)

    Claudia eKnief

    2015-12-01

    Full Text Available Methane-oxidizing bacteria are characterized by their capability to grow on methane as sole source of carbon and energy. Cultivation-dependent and –independent methods have revealed that this functional guild of bacteria comprises a substantial diversity of organisms. In particular the use of cultivation-independent methods targeting a subunit of the particulate methane monooxygenase (pmoA as functional marker for the detection of aerobic methanotrophs has resulted in thousands of sequences representing unknown methanotrophic bacteria. This limits data interpretation due to restricted information about these uncultured methanotrophs. A few groups of uncultivated methanotrophs are assumed to play important roles in methane oxidation in specific habitats, while the biology behind other sequence clusters remains still largely unknown. The discovery of evolutionary related monooxygenases in non-methanotrophic bacteria and of pmoA paralogs in methanotrophs requires that sequence clusters of uncultivated organisms have to be interpreted with care. This review article describes the present diversity of cultivated and uncultivated aerobic methanotrophic bacteria based on pmoA gene sequence diversity. It summarizes current knowledge about cultivated and major clusters of uncultivated methanotrophic bacteria and evaluates habitat specificity of these bacteria at different levels of taxonomic resolution. Habitat specificity exists for diverse lineages and at different taxonomic levels. Methanotrophic genera such as Methylocystis and Methylocaldum are identified as generalists, but they harbor habitat specific methanotrophs at species level. This finding implies that future studies should consider these diverging preferences at different taxonomic levels when analyzing methanotrophic communities.

  6. Diversity and Habitat Preferences of Cultivated and Uncultivated Aerobic Methanotrophic Bacteria Evaluated Based on pmoA as Molecular Marker

    Science.gov (United States)

    Knief, Claudia

    2015-01-01

    Methane-oxidizing bacteria are characterized by their capability to grow on methane as sole source of carbon and energy. Cultivation-dependent and -independent methods have revealed that this functional guild of bacteria comprises a substantial diversity of organisms. In particular the use of cultivation-independent methods targeting a subunit of the particulate methane monooxygenase (pmoA) as functional marker for the detection of aerobic methanotrophs has resulted in thousands of sequences representing “unknown methanotrophic bacteria.” This limits data interpretation due to restricted information about these uncultured methanotrophs. A few groups of uncultivated methanotrophs are assumed to play important roles in methane oxidation in specific habitats, while the biology behind other sequence clusters remains still largely unknown. The discovery of evolutionary related monooxygenases in non-methanotrophic bacteria and of pmoA paralogs in methanotrophs requires that sequence clusters of uncultivated organisms have to be interpreted with care. This review article describes the present diversity of cultivated and uncultivated aerobic methanotrophic bacteria based on pmoA gene sequence diversity. It summarizes current knowledge about cultivated and major clusters of uncultivated methanotrophic bacteria and evaluates habitat specificity of these bacteria at different levels of taxonomic resolution. Habitat specificity exists for diverse lineages and at different taxonomic levels. Methanotrophic genera such as Methylocystis and Methylocaldum are identified as generalists, but they harbor habitat specific methanotrophs at species level. This finding implies that future studies should consider these diverging preferences at different taxonomic levels when analyzing methanotrophic communities. PMID:26696968

  7. A rapid and convenient method for fluorescence analysis of in vitro cultivated metacestode vesicles from Echinococcus multilocularis.

    Directory of Open Access Journals (Sweden)

    Zhe Cheng

    Full Text Available We here describe a convenient method for preparation, fixation and fluorescence analysis of in vitro cultivated metacestode vesicles from E. multilocularis. Parasite materials could be prepared in one hour, did not need to be sectioned, and were subsequently utilized for further whole-mount staining assays directly. Using these preparations, in combination with conventional fluorescence staining techniques, we could detect the expression and subcellular localization of a specific protein and identify in situ proliferative or apoptotic cells in the germinal layer of metacestode vesicles. Based on this approach, future molecular and cellular analysis of Echinococcus metacestode vesicles in the in vitro system will be greatly facilitated.

  8. Simple and efficient method for isolation and cultivation of endoscopically obtained human colonocytes

    DEFF Research Database (Denmark)

    Seidelin, Jakob B; Horn, Thomas; Nielsen, Ole H

    2003-01-01

    Few comparative and validated reports exist on the isolation and growth of colonoscopically obtained colonic epithelium. The aim of this study was to develop and validate a simple method for the cultivation of colonoscopically obtained colonocytes. Forty patients, who underwent routine colonoscopy...... and where the diagnosis of irritable bowel syndrome was later reached, were included. Seven colon biopsies were taken and incubated at varying time periods of 10-120 min and temperatures of 4-37 degrees C in a chelating buffer. The epithelium was then harvested and cultivated under three different...... conditions: 1) on a collagen coating, 2) embedded in a collagen gel, or 3) embedded in a gel put on a porous well insert. The effect of conditioned medium (CM), insulin, transferrin, selenium, and the oxygen content was assessed. Viability was tested by the metabolic dimethylthiazol...

  9. Seasonal cultivated and fallow cropland mapping using MODIS-based automated cropland classification algorithm

    Science.gov (United States)

    Wu, Zhuoting; Thenkabail, Prasad S.; Mueller, Rick; Zakzeski, Audra; Melton, Forrest; Johnson, Lee; Rosevelt, Carolyn; Dwyer, John; Jones, Jeanine; Verdin, James P.

    2013-01-01

    Increasing drought occurrences and growing populations demand accurate, routine, and consistent cultivated and fallow cropland products to enable water and food security analysis. The overarching goal of this research was to develop and test automated cropland classification algorithm (ACCA) that provide accurate, consistent, and repeatable information on seasonal cultivated as well as seasonal fallow cropland extents and areas based on the Moderate Resolution Imaging Spectroradiometer remote sensing data. Seasonal ACCA development process involves writing series of iterative decision tree codes to separate cultivated and fallow croplands from noncroplands, aiming to accurately mirror reliable reference data sources. A pixel-by-pixel accuracy assessment when compared with the U.S. Department of Agriculture (USDA) cropland data showed, on average, a producer’s accuracy of 93% and a user’s accuracy of 85% across all months. Further, ACCA-derived cropland maps agreed well with the USDA Farm Service Agency crop acreage-reported data for both cultivated and fallow croplands with R-square values over 0.7 and field surveys with an accuracy of ≥95% for cultivated croplands and ≥76% for fallow croplands. Our results demonstrated the ability of ACCA to generate cropland products, such as cultivated and fallow cropland extents and areas, accurately, automatically, and repeatedly throughout the growing season.

  10. Water Productivity of Irrigated Rice under Transplanting, Wet Seeding and Dry Seeding Methods of Cultivation

    Directory of Open Access Journals (Sweden)

    Murali, NS.

    1997-01-01

    Full Text Available Water productivity (WP of irrigated lowland rice was determined during the 1994 dry (January to May and wet (August to December seasons on a heavy clay acid sulphate soil. Treatments consisted of three cultivation methods : transplanted rice, pregerminated seeds broadcasted on puddled soil (wet seeding and dry seeds broadcasted on unpuddled soil (dry seeding. In wet and dry seeded plots, continuous standing water condition was initiated 17 days after sowing. Total water requirement for rice production was highest in transplanted plots (755 mm in wet season and 1154 mm in dry season and was lowest in dry seeded plots (505 mm in wet season and 1040 mm in dry season. Dry seeding required no water for land preparation but transplanting and wet seeding methods required 18 - 20 % of total water requirement in dry season and 27 - 29 % in wet season. Total percolation was maximum (99 mm in wet season and 215 mm in dry season in dry seeding method and was minimum (62 mm in wet season and 94 mm in dry season in transplanting method. In dry and wet seeding methods, daily percolation gradually decreased with the age of the crop. Total seepage loss did not show any significant difference between the cultivation methods in the two seasons. Grain yield was not affected by the three cultivation methods in both seasons. Water productivity (the ratio between grain yield and total amount of water used in production was 3.5 - 4.1 kg ha-1 mm-1, 3.8 - 4.4 kg ha-1 mm-1 and 4.1 - 5.5 kg ha-1 mm-1 in transplanted, wet seeded and dry seeded rice, respectively. Labour requirement for land preparation and sowing was maximum in transplanted (219 - 226 man-hours ha-1 followed by wet (104 -112 man-hours ha-1 and dry seeded (94 - 99 man-hours ha-1 methods. However, in wet season extra labour (77 man-hours ha-1 was required for weeding after crop establishment in dry and wet seeding methods. Crop maturity was 20 days earlier in wet and dry seeding methods compared to

  11. 一种分离培养硫酸盐还原菌的改进方法%A IMPROVED METHOD FOR ISLATION AND CULTIVATION OF SULFATE-REDUCING BACTERIA

    Institute of Scientific and Technical Information of China (English)

    万海清; 苏仕军; 葛长海; 万雪松; 朱家骅

    2003-01-01

    On the base of the characteristics of sulfate-reducing bacteria (SRB) growth and dish cultivated method, a new method for isolation and cultivation of SRB is established. It is dilution spread and repeat dish sandwish cultivated method with the exellence of dilution spread and embeded growth, and it can help separation and identification of SRB. The method has simple operation and less equipment. And using this method, the natural SRB drop was successfully gained, and small agar pieces containing SRB drop were acquired in easiness. The brief SRB transfering operation in strict anaerobic condition was in-deed realized. Fig 1, Ref 7.

  12. Cultivation-independent methods reveal differences among bacterial gut microbiota in triatomine vectors of Chagas disease.

    Directory of Open Access Journals (Sweden)

    Fabio Faria da Mota

    Full Text Available BACKGROUND: Chagas disease is a trypanosomiasis whose agent is the protozoan parasite Trypanosoma cruzi, which is transmitted to humans by hematophagous bugs known as triatomines. Even though insecticide treatments allow effective control of these bugs in most Latin American countries where Chagas disease is endemic, the disease still affects a large proportion of the population of South America. The features of the disease in humans have been extensively studied, and the genome of the parasite has been sequenced, but no effective drug is yet available to treat Chagas disease. The digestive tract of the insect vectors in which T. cruzi develops has been much less well investigated than blood from its human hosts and constitutes a dynamic environment with very different conditions. Thus, we investigated the composition of the predominant bacterial species of the microbiota in insect vectors from Rhodnius, Triatoma, Panstrongylus and Dipetalogaster genera. METHODOLOGY/PRINCIPAL FINDINGS: Microbiota of triatomine guts were investigated using cultivation-independent methods, i.e., phylogenetic analysis of 16s rDNA using denaturing gradient gel electrophoresis (DGGE and cloned-based sequencing. The Chao index showed that the diversity of bacterial species in triatomine guts is low, comprising fewer than 20 predominant species, and that these species vary between insect species. The analyses showed that Serratia predominates in Rhodnius, Arsenophonus predominates in Triatoma and Panstrongylus, while Candidatus Rohrkolberia predominates in Dipetalogaster. CONCLUSIONS/SIGNIFICANCE: The microbiota of triatomine guts represents one of the factors that may interfere with T. cruzi transmission and virulence in humans. The knowledge of its composition according to insect species is important for designing measures of biological control for T. cruzi. We found that the predominant species of the bacterial microbiota in triatomines form a group of low

  13. An Inquiry into the Cultivation of Students' Creative Learning Method%培养学生创造性的学习方法

    Institute of Scientific and Technical Information of China (English)

    谭美玲

    2011-01-01

    高等学校是培养学生创造性精神和实用型人才的基地,是科学文化发展和促进和谐社会建设的重要场所。高校要通过不断的教学改革,培养学生的素质教养,努力培养学生的创造性思维。本文主要是介绍创造的内涵、培养创造性学习方法的意义及如何培养创造性学习方法等方面内容。%The college is a base of cultivating students' creative spirit and cultivating practical talents,and it is also an important position of developing scientific culture and promoting the construction of harmonious society.The college should cultivate students' quality and their creative thinking through constant teaching reforms.This paper mainly introduces the definition of creativity,the meaning of cultivating creative learning method and how to cultivate creative learning method.

  14. Crossing Methods and Cultivation Conditions for Rapid Production of Segregating Populations in Three Grain Amaranth Species.

    Science.gov (United States)

    Stetter, Markus G; Zeitler, Leo; Steinhaus, Adrian; Kroener, Karoline; Biljecki, Michelle; Schmid, Karl J

    2016-01-01

    Grain amaranths (Amaranthus spp.) have been cultivated for thousands of years in Central and South America. Their grains are of high nutritional value, but the low yield needs to be increased by selection of superior genotypes from genetically diverse breeding populations. Amaranths are adapted to harsh conditions and can be cultivated on marginal lands although little is known about their physiology. The development of controlled growing conditions and efficient crossing methods is important for research on and improvement of this ancient crop. Grain amaranth was domesticated in the Americas and is highly self-fertilizing with a large inflorescence consisting of thousands of very small flowers. We evaluated three different crossing methods (open pollination, hot water emasculation and hand emasculation) for their efficiency in amaranth and validated them with genetic markers. We identified cultivation conditions that allow an easy control of flowering time by day length manipulation and achieved flowering times of 4 weeks and generation times of 2 months. All three different crossing methods successfully produced hybrid F1 offspring, but with different success rates. Open pollination had the lowest (10%) and hand emasculation the highest success rate (74%). Hot water emasculation showed an intermediate success rate (26%) with a maximum of 94% success. It is simple to perform and suitable for a more large-scale production of hybrids. We further evaluated 11 single nucleotide polymorphism (SNP) markers and found that they were sufficient to validate all crosses of the genotypes used in this study for intra- and interspecific hybridizations. Despite its very small flowers, crosses in amaranth can be carried out efficiently and evaluated with inexpensive SNP markers. Suitable growth conditions strongly reduce the generation time and allow the control of plant height, flowering time, and seed production. In combination, this enables the rapid production of segregating

  15. Crossing Methods and Cultivation Conditions for Rapid Production of Segregating Populations in Three Grain Amaranth Species.

    Science.gov (United States)

    Stetter, Markus G; Zeitler, Leo; Steinhaus, Adrian; Kroener, Karoline; Biljecki, Michelle; Schmid, Karl J

    2016-01-01

    Grain amaranths (Amaranthus spp.) have been cultivated for thousands of years in Central and South America. Their grains are of high nutritional value, but the low yield needs to be increased by selection of superior genotypes from genetically diverse breeding populations. Amaranths are adapted to harsh conditions and can be cultivated on marginal lands although little is known about their physiology. The development of controlled growing conditions and efficient crossing methods is important for research on and improvement of this ancient crop. Grain amaranth was domesticated in the Americas and is highly self-fertilizing with a large inflorescence consisting of thousands of very small flowers. We evaluated three different crossing methods (open pollination, hot water emasculation and hand emasculation) for their efficiency in amaranth and validated them with genetic markers. We identified cultivation conditions that allow an easy control of flowering time by day length manipulation and achieved flowering times of 4 weeks and generation times of 2 months. All three different crossing methods successfully produced hybrid F1 offspring, but with different success rates. Open pollination had the lowest (10%) and hand emasculation the highest success rate (74%). Hot water emasculation showed an intermediate success rate (26%) with a maximum of 94% success. It is simple to perform and suitable for a more large-scale production of hybrids. We further evaluated 11 single nucleotide polymorphism (SNP) markers and found that they were sufficient to validate all crosses of the genotypes used in this study for intra- and interspecific hybridizations. Despite its very small flowers, crosses in amaranth can be carried out efficiently and evaluated with inexpensive SNP markers. Suitable growth conditions strongly reduce the generation time and allow the control of plant height, flowering time, and seed production. In combination, this enables the rapid production of segregating

  16. Crossing methods and cultivation conditions for rapid production of segregating populations in three grain amaranth species

    Directory of Open Access Journals (Sweden)

    Markus G Stetter

    2016-06-01

    Full Text Available Grain amaranths (Amaranthus spp. have been cultivated for thousands of years in Central and South America. Their grains are of high nutritional value, but the low yield needs to be increased by selection of superior genotypes from genetically diverse breeding populations. Amaranths are adapted to harsh conditions and can be cultivated on marginal lands although little is known about their physiology. The development of controlled growing conditions and efficient crossing methods is important for research on and improvement of this ancient crop. Grain amaranth was domesticated in the Americas and is highly self-fertilizing with a large inflorescence consisting of thousands of very small flowers. We evaluated three different crossing methods (open pollination, hot water emasculation and hand emasculation for their efficiency in amaranth and validated them with genetic markers. We identified cultivation conditions that allow an easy control of flowering time by manipulating day length and achieved flowering times of four weeks and generation times of two months. All three different crossing methods successfully produced hybrid F1 offspring, but with different success rates. Open pollination had the lowest (10% and hand emasculation the highest success rate (74%. Hot water emasculation showed an intermediate success rate (26% with a maximum of 94% success. It is simple to perform and suitable for a more large-scale production of hybrids. We further evaluated 11 single nucleotide polymorphism (SNP markers and found that they were sufficient to validate all crosses of the genotypes used in this study for intra- and interspecific hybridisations. Despite its very small flowers, crosses in amaranth can be carried out efficiently and evaluated with inexpensive SNP markers. Suitable growth conditions strongly reduce the generation time and allow the control of plant height, flowering time and seed production. In combination, this enables the rapid

  17. Genetic structure of cultivated flax (Linum usitatissimum L. based on retrotransposon-based markers

    Directory of Open Access Journals (Sweden)

    Habibollahi Hadi

    2015-01-01

    Full Text Available Flax (Linum usitatissimum L. is one of the most important fiber and oil crop plants cultivated since ancient time. The flax seeds contain high amount of omega- 3-fatty acids and biologically active lignans. In spite of economic importance of cultivated flax, no information is available on its genetic variability and population structure in Iran. Therefore, we used six inter-retrotransposon amplified polymorphism (IRAP markers and 15 combined IRAP markers to reveal within and among population genetic diversity in this crop plant. We used 30 randomly selected plants in three geographical populations for present investigation. AMOVA test produced significant genetic difference (PhiPT = 0.40, P = 0.010 among the studied populations and also revealed that, 40% of total genetic variability was due to within population diversity while, 60% was due to among population genetic differentiation. Gst (0.78, P = 0.001, Hedrick, standardised fixation index (G'st = 0.83, P = 0.001, revealed that the studied populations are genetically differentiated. STRUCTURE plot based on admixture model revealed that the studied populations differed extensively in their genetic content, but some degree of shared alleles occurred between them. Some adaptive IRAP loci were identified by LFMM analysis. These loci were private alleles restricted to geographical populations. Data obtained may be used in breeding and hybridization program of flax in the country.

  18. On Cultivation Methods of College Student Laboratory Assistants Based on Self-managing Mode%基于自主管理模式的高校机房学生管理员培养方法研究

    Institute of Scientific and Technical Information of China (English)

    卢荣辉; 梁洪涛

    2011-01-01

    计算机机房是高校重要的实践教学场所,用户对机房的服务需求不断提高。通过自适应学习能力、团队协作能力的培养,建设了一支具有自我管理、自主学习能力的学生管理员队伍。学生管理员协助老师提高机房管理和维护效率的同时,其自身的计算机专业实践能力和综合素质得到了提高。%With computer laboratory becoming an important place of practical teaching in college,user's requirements of service provided have also increased greatly.Through the cultivation of their adaptive learning ability and team working ability,an efficient team of student lab assistants can be built who are good at self-management and self-learning.In this way,students can not only help teachers improve the efficiency of laboratory management and maintenance,but also improve their own practical abilities in computer and integrated qualities.

  19. On the Cultivation of Automation Majors' Research Innovation Ability Based on Scientific Research Projects

    Science.gov (United States)

    Wang, Lipeng; Li, Mingqiu

    2012-01-01

    Currently, it has become a fundamental goal for the engineering major to cultivate high-quality engineering technicians with innovation ability in scientific research which is an important academic ability necessary for them. This paper mainly explores the development of comprehensive and designing experiments in automation based on scientific…

  20. Work-Based Research Degrees: Systematic Cultivation through a University-Industry Network Space

    Science.gov (United States)

    Sense, Andrew J.

    2016-01-01

    The purpose of this paper is to expound on an innovative approach to cultivating work-based Doctorates and Masters of Philosophy degrees, which involves close collaboration between industry organisations and a tertiary institution. This paper also reports on an examination of the programme's structural ability to help develop the learning…

  1. Paper-based device for separation and cultivation of single microalga.

    Science.gov (United States)

    Chen, Chih-Chung; Liu, Yi-Ju; Yao, Da-Jeng

    2015-12-01

    Single-cell separation is among the most useful techniques in biochemical research, diagnosis and various industrial applications. Microalgae species have great economic importance as industrial raw materials. Microalgae species collected from environment are typically a mixed and heterogeneous population of species that must be isolated and purified for examination and further application. Conventional methods, such as serial dilution and a streaking-plate method, are intensive of labor and inefficient. We developed a paper-based device for separation and cultivation of single microalga. The fabrication was simply conducted with a common laser printer and required only a few minutes without lithographic instruments and clean-room. The driving force of the paper device was simple capillarity without a complicated pump connection that is part of most devices for microfluidics. The open-structure design of the paper device makes it operable with a common laboratory micropipette for sample transfer and manipulation with a naked eye or adaptable to a robotic system with functionality of high-throughput retrieval and analysis. The efficiency of isolating a single cell from mixed microalgae species is seven times as great as with a conventional method involving serial dilution. The paper device can serve also as an incubator for microalgae growth on simply rinsing the paper with a growth medium. Many applications such as highly expressed cell selection and various single-cell analysis would be applicable. PMID:26459444

  2. Paper-based device for separation and cultivation of single microalga.

    Science.gov (United States)

    Chen, Chih-Chung; Liu, Yi-Ju; Yao, Da-Jeng

    2015-12-01

    Single-cell separation is among the most useful techniques in biochemical research, diagnosis and various industrial applications. Microalgae species have great economic importance as industrial raw materials. Microalgae species collected from environment are typically a mixed and heterogeneous population of species that must be isolated and purified for examination and further application. Conventional methods, such as serial dilution and a streaking-plate method, are intensive of labor and inefficient. We developed a paper-based device for separation and cultivation of single microalga. The fabrication was simply conducted with a common laser printer and required only a few minutes without lithographic instruments and clean-room. The driving force of the paper device was simple capillarity without a complicated pump connection that is part of most devices for microfluidics. The open-structure design of the paper device makes it operable with a common laboratory micropipette for sample transfer and manipulation with a naked eye or adaptable to a robotic system with functionality of high-throughput retrieval and analysis. The efficiency of isolating a single cell from mixed microalgae species is seven times as great as with a conventional method involving serial dilution. The paper device can serve also as an incubator for microalgae growth on simply rinsing the paper with a growth medium. Many applications such as highly expressed cell selection and various single-cell analysis would be applicable.

  3. Support system based on GIS and weighted sum method for drawing up of land suitability map for agriculture. Application to durum wheat cultivation in the area of Mleta (Algeria)

    Energy Technology Data Exchange (ETDEWEB)

    Mendas, A.; Delali, A.

    2012-11-01

    Due to constant decrease in farmlands, it is important to identify the best lands for sustainable agriculture (productive and profitable agriculture that protects the environment and that is socially equitable). This requirement has resulted in the development of land suitability maps for agriculture by combining a range of factors. Spatial analysis approaches, based on the concepts of the weighted sum, combined with Geographical Information Systems (GIS) offer the opportunity to efficiently produce these land suitability maps. The functions of the weighted sum make it possible to assign numerical weights, to distinguish between positive and negative criteria and to rank alternatives. A spatial decision support system has been developed for establishing the land suitability map for agriculture. It incorporates a version of the weighted sum method SAW (Simple Additive Weighting), applicable to the vector data model, in ArcGIS within the GIS program package environment. This approach has been tested on the area of Mleta (Algeria) to assess the land suitability for durum wheat agriculture. The parameters and the classification system used in this work are inspired from the FAO. The coherence of results confirms the system effectiveness. (Author) 23 refs.

  4. Preferences based Control Design of Complex Fed-batch Cultivation Process

    Directory of Open Access Journals (Sweden)

    Yuri Pavlov

    2009-08-01

    Full Text Available In the paper is presented preferences based control design and stabilization of the growth rate of fed-batch cultivation processes. The control is based on an enlarged Wang-Monod-Yerusalimsky kinetic model. Expected utility theory is one of the approaches for utilization of conceptual information (expert preferences. In the article is discussed utilization of stochastic machine learning procedures for evaluation of expert utilities as criteria for optimization.

  5. Characteristics of Eastern Canadian cultivated Sphagnum and potential use as a substitute for perlite and vermiculite in peat-based horticultural substrates

    OpenAIRE

    M. Aubé; M. Quenum; L.L. Ranasinghe

    2015-01-01

    Sphagnum cultivation on harvested peatlands to meet wetland restoration objectives could be an economically feasible activity since cultivated Sphagnum has potential horticultural applications. We compared the characteristics of cultivated Sphagnum from Shippagan (Canada) with those of non-cultivated Sphagnum products from Chile, New Zealand and Canada, and assessed its potential as a perlite and vermiculite substitute in horticultural peat-based substrates. Shippagan cultivated Sphagnum was ...

  6. A Study on Cultivation of Students’Autonomous Learning Ability by Project-Based Teaching Method in the Indepen-dent College%项目教学法培养独立学院学生英语自主学习能力初探

    Institute of Scientific and Technical Information of China (English)

    孙远用

    2015-01-01

    该文探索项目教学法在地方性高校大学英语教学中的应用,通过项目式学习,让学生参与学习内容的制定和选择,学习效果的评价,项目的组织等活动,提高用语言解决实际问题的能力,进而培养学生自主学习能力。%This paper tries to explore the application of Project-Based Teaching Method in college English teaching in the local colleges. Through project-based learning, students are expected to participate in the teaching activities, such as choosing and se⁃lecting teaching materials, formative assessment, and organization of the project, in order to promote students ’ability to solve prac⁃tical problems in target language and to cultivate students’autonomous learning ability.

  7. Genetic structure of cultivated flax (Linum usitatissimum L.) based on retrotransposon-based markers

    OpenAIRE

    Habibollahi Hadi; Noormohammadi Zahra; Sheidai Masoud; Farahani Farah

    2015-01-01

    Flax (Linum usitatissimum L.) is one of the most important fiber and oil crop plants cultivated since ancient time. The flax seeds contain high amount of omega- 3-fatty acids and biologically active lignans. In spite of economic importance of cultivated flax, no information is available on its genetic variability and population structure in Iran. Therefore, we used six inter-retrotransposon amplified polymorphism (IRAP) markers and 15 combined IRAP markers ...

  8. A Comparative Accuracy Analysis of Classification Methods in Determination of Cultivated Lands with Spot 5 Satellite Imagery

    Science.gov (United States)

    kaya, S.; Alganci, U.; Sertel, E.; Ustundag, B.

    2013-12-01

    A Comparative Accuracy Analysis of Classification Methods in Determination of Cultivated Lands with Spot 5 Satellite Imagery Ugur ALGANCI1, Sinasi KAYA1,2, Elif SERTEL1,2,Berk USTUNDAG3 1 ITU, Center for Satellite Communication and Remote Sensing, 34469, Maslak-Istanbul,Turkey 2 ITU, Department of Geomatics, 34469, Maslak-Istanbul, Turkey 3 ITU, Agricultural and Environmental Informatics Research Center,34469, Maslak-Istanbul,Turkey alganci@itu.edu.tr, kayasina@itu.edu.tr, sertele@itu.edu.tr, berk@berk.tc ABSTRACT Cultivated land determination and their area estimation are important tasks for agricultural management. Derived information is mostly used in agricultural policies and precision agriculture, in specifically; yield estimation, irrigation and fertilization management and farmers declaration verification etc. The use of satellite image in crop type identification and area estimate is common for two decades due to its capability of monitoring large areas, rapid data acquisition and spectral response to crop properties. With launch of high and very high spatial resolution optical satellites in the last decade, such kind of analysis have gained importance as they provide information at big scale. With increasing spatial resolution of satellite images, image classification methods to derive the information form them have become important with increase of the spectral heterogeneity within land objects. In this research, pixel based classification with maximum likelihood algorithm and object based classification with nearest neighbor algorithm were applied to 2012 dated 2.5 m resolution SPOT 5 satellite images in order to investigate the accuracy of these methods in determination of cotton and corn planted lands and their area estimation. Study area was selected in Sanliurfa Province located on Southeastern Turkey that contributes to Turkey's agricultural production in a major way. Classification results were compared in terms of crop type identification using

  9. Efficient animal-serum free 3D cultivation method for adult human neural crest-derived stem cell therapeutics

    Directory of Open Access Journals (Sweden)

    JFW Greiner

    2011-12-01

    Full Text Available Due to their broad differentiation potential and their persistence into adulthood, human neural crest-derived stem cells (NCSCs harbour great potential for autologous cellular therapies, which include the treatment of neurodegenerative diseases and replacement of complex tissues containing various cell types, as in the case of musculoskeletal injuries. The use of serum-free approaches often results in insufficient proliferation of stem cells and foetal calf serum implicates the use of xenogenic medium components. Thus, there is much need for alternative cultivation strategies. In this study we describe for the first time a novel, human blood plasma based semi-solid medium for cultivation of human NCSCs. We cultivated human neural crest-derived inferior turbinate stem cells (ITSCs within a blood plasma matrix, where they revealed higher proliferation rates compared to a standard serum-free approach. Three-dimensionality of the matrix was investigated using helium ion microscopy. ITSCs grew within the matrix as revealed by laser scanning microscopy. Genetic stability and maintenance of stemness characteristics were assured in 3D cultivated ITSCs, as demonstrated by unchanged expression profile and the capability for self-renewal. ITSCs pre-cultivated in the 3D matrix differentiated efficiently into ectodermal and mesodermal cell types, particularly including osteogenic cell types. Furthermore, ITSCs cultivated as described here could be easily infected with lentiviruses directly in substrate for potential tracing or gene therapeutic approaches. Taken together, the use of human blood plasma as an additive for a completely defined medium points towards a personalisable and autologous cultivation of human neural crest-derived stem cells under clinical grade conditions.

  10. Diversity and Habitat Preferences of Cultivated and Uncultivated Aerobic Methanotrophic Bacteria Evaluated Based on pmoA as Molecular Marker

    OpenAIRE

    Knief, Claudia

    2015-01-01

    Methane-oxidizing bacteria are characterized by their capability to grow on methane as sole source of carbon and energy. Cultivation-dependent and -independent methods have revealed that this functional guild of bacteria comprises a substantial diversity of organisms. In particular the use of cultivation-independent methods targeting a subunit of the particulate methane monooxygenase (pmoA) as functional marker for the detection of aerobic methanotrophs has resulted in thousands of sequences ...

  11. Online Supervision of Penicillin Cultivations Based on Rolling MPCA%基于滚动MPCA的青霉素发酵过程的在线监控

    Institute of Scientific and Technical Information of China (English)

    汪志锋; 袁景淇

    2007-01-01

    To reduce the variations of the production process in penicillin cultivations, a rolling multivariate statistical approach based on multiway principle component analysis (MPCA) is developed and used for fault diagnosis of penicillin cultivations. Using the moving data windows technique, the static MPCA is extended for use in dynamic process performance monitoring. The control chart is set up using the historical data collected from the past successful batches, thereby resulting in simplification of monitoring charts, easy tracking of the progress in each batch run, and monitoring the occurrence of the observable upsets. Data from the commercial-scale penicillin fermentation process are used to develop the rolling model. Using this method, faults are detected in real time and the corresponding measurements of these faults are directly made through inspection of a few simple plots (t-chart,SPE-chart, and T2-chart). Thus, the present methodology allows the process operator to actively monitor the data from several cultivations simultaneously.

  12. Cultivation-based multiplex phenotyping of human gut microbiota allows targeted recovery of previously uncultured bacteria

    DEFF Research Database (Denmark)

    Rettedal, Elizabeth; Gumpert, Heidi; Sommer, Morten

    2014-01-01

    The human gut microbiota is linked to a variety of human health issues and implicated in antibiotic resistance gene dissemination. Most of these associations rely on culture-independent methods, since it is commonly believed that gut microbiota cannot be easily or sufficiently cultured. Here, we...... show that carefully designed conditions enable cultivation of a representative proportion of human gut bacteria, enabling rapid multiplex phenotypic profiling. We use this approach to determine the phylogenetic distribution of antibiotic tolerance phenotypes for 16 antibiotics in the human gut...

  13. Analysis of rhizosphere bacteria of rice cultivated in Andosol lowland and upland fields using molecular biological methods

    OpenAIRE

    DOI, Tetsuya; Hagiwara, Yusuke; Abe, Jun; Morita, Shigenori

    2007-01-01

    Bacteria in the rhizosphere influence plant growth and interact with plant roots. Microscopy and culture method have been used for studies of microorganisms of the rhizosphere, but these methods are insufficient for evaluation because most rhizosphere bacteria are viable but non-culturable (VBNC). Bacteria in the rhizosphere of rice cultivated in Andosol lowland and upland fields were analyzed in this study using PCR-DGGE and FISH, in combination with modified pretreatments. Results show that...

  14. Secretome data from Trichoderma reesei and Aspergillus niger cultivated in submerged and sequential fermentation methods.

    Science.gov (United States)

    Florencio, Camila; Cunha, Fernanda M; Badino, Alberto C; Farinas, Cristiane S; Ximenes, Eduardo; Ladisch, Michael R

    2016-09-01

    The cultivation procedure and the fungal strain applied for enzyme production may influence levels and profile of the proteins produced. The proteomic analysis data presented here provide critical information to compare proteins secreted by Trichoderma reesei and Aspergillus niger when cultivated through submerged and sequential fermentation processes, using steam-explosion sugarcane bagasse as inducer for enzyme production. The proteins were organized according to the families described in CAZy database as cellulases, hemicellulases, proteases/peptidases, cell-wall-protein, lipases, others (catalase, esterase, etc.), glycoside hydrolases families, predicted and hypothetical proteins. Further detailed analysis of this data is provided in "Secretome analysis of Trichoderma reesei and Aspergillus niger cultivated by submerged and sequential fermentation process: enzyme production for sugarcane bagasse hydrolysis" C. Florencio, F.M. Cunha, A.C Badino, C.S. Farinas, E. Ximenes, M.R. Ladisch (2016) [1]. PMID:27419196

  15. Can agricultural Cultivation Methods Influence the Healthfulness of Crops for Foods?

    DEFF Research Database (Denmark)

    Melballe Jensen, Maja; Jørgensen, Henry; Halekoh, Ulrich;

    2012-01-01

    . Additionally, the nutritional quality was affected by harvest year and location. However, harvest year and location rather than cultivation system affected the measured health biomarkers. In conclusion, the differences in dietary treatments composed of ingredients from different cultivation systems did......The aim of the current study was to investigate if there are any health effects of long-term consumption of organically grown crops using a rat model. Crops were retrieved over two years from a long-term field trial at three different locations in Denmark, using three different cultivation systems...... not lead to significant differences in the measured health biomarkers, except for a significant difference in plasma IgG levels....

  16. Secretome data from Trichoderma reesei and Aspergillus niger cultivated in submerged and sequential fermentation methods

    Directory of Open Access Journals (Sweden)

    Camila Florencio

    2016-09-01

    Full Text Available The cultivation procedure and the fungal strain applied for enzyme production may influence levels and profile of the proteins produced. The proteomic analysis data presented here provide critical information to compare proteins secreted by Trichoderma reesei and Aspergillus niger when cultivated through submerged and sequential fermentation processes, using steam-explosion sugarcane bagasse as inducer for enzyme production. The proteins were organized according to the families described in CAZy database as cellulases, hemicellulases, proteases/peptidases, cell-wall-protein, lipases, others (catalase, esterase, etc., glycoside hydrolases families, predicted and hypothetical proteins. Further detailed analysis of this data is provided in “Secretome analysis of Trichoderma reesei and Aspergillus niger cultivated by submerged and sequential fermentation process: enzyme production for sugarcane bagasse hydrolysis” C. Florencio, F.M. Cunha, A.C Badino, C.S. Farinas, E. Ximenes, M.R. Ladisch (2016 [1].

  17. Molecular identification based on ITS sequences for Kappaphycus and Eucheuma cultivated in China

    Institute of Scientific and Technical Information of China (English)

    ZHAO Sufen; HE Peimin

    2011-01-01

    The systematic classification of the Eucheurnatoideae is difficult because of their variable morphology and interpretation of reproductive structures.Kappaphycus and Eucheuma specimens cultivated on the Hainan and Fujian coast of China were introduced from Vietnam,the Philippines and Indonesia.Combined with morphological characteristics,all Kappaphycus and Eucheuma cultivated strains were identified by internal transcribed spacer (ITS) sequences.The phylogenetic tree was constructed using neighbor-joining and maximum likelihood methods.The results indicate that different ITS sequence lengths occurred in the different genera and species.An obvious difference in morphology could be found in the protuberance shape between Kappaphycus and Eucheuma.The protuberance in Eucheuma was thorn-like and in Kappaphycus was wartlike or papillate.Their ITS sequence lengths differed significantly in nucleotide variation rates up to 58.55%-63.90%.All nucleotide variations occurred in the ITS1 andITS2 regions except for five nucleotide transversions in the 5.8S rDNA region.In addition,the difference was at the branches among congeneric species.Kappaphycus sp.had branches with small buds,while K.alvarezii did not have such a feature.The nucleotide variation rates varied from 7.02% to 7.48% among species; within the same species of the clades it was <1.20%.Eucheumatoideae algae cultivated in China consisted of three clades,K.alvarezii,Kappaphycus sp.,and E.denticulatum.The results indicate that ITS sequence analysis was an effective way for identification of interspecies and intraspecies phylogenetic relationships and might provide a clue for molecular identification of algal Eucheumatoideae.

  18. Genetic diversity of cultivated flax (Linum usitatissimum L.) germplasm assessed by retrotransposon-based markers.

    Science.gov (United States)

    Smýkal, P; Bačová-Kerteszová, N; Kalendar, R; Corander, J; Schulman, A H; Pavelek, M

    2011-05-01

    Retrotransposon segments were characterized and inter-retrotransposon amplified polymorphism (IRAP) markers developed for cultivated flax (Linum usitatissimum L.) and the Linum genus. Over 75 distinct long terminal repeat retrotransposon segments were cloned, the first set for Linum, and specific primers designed for them. IRAP was then used to evaluate genetic diversity among 708 accessions of cultivated flax comprising 143 landraces, 387 varieties, and 178 breeding lines. These included both traditional and modern, oil (86), fiber (351), and combined-use (271) accessions, originating from 36 countries, and 10 wild Linum species. The set of 10 most polymorphic primers yielded 141 reproducible informative data points per accession, with 52% polymorphism and a 0.34 Shannon diversity index. The maximal genetic diversity was detected among wild Linum species (100% IRAP polymorphism and 0.57 Jaccard similarity), while diversity within cultivated germplasm decreased from landraces (58%, 0.63) to breeding lines (48%, 0.85) and cultivars (50%, 0.81). Application of Bayesian methods for clustering resulted in the robust identification of 20 clusters of accessions, which were unstratified according to origin or user type. This indicates an overlap in genetic diversity despite disruptive selection for fiber versus oil types. Nevertheless, eight clusters contained high proportions (70-100%) of commercial cultivars, whereas two clusters were rich (60%) in landraces. These findings provide a basis for better flax germplasm management, core collection establishment, and exploration of diversity in breeding, as well as for exploration of the role of retrotransposons in flax genome dynamics. PMID:21293839

  19. High-power light-emitting diode based facility for plant cultivation

    Energy Technology Data Exchange (ETDEWEB)

    Tamulaitis, G [Institute of Materials Science and Applied Research, Vilnius University, Sauletekio al. 9-III, LT-10222 Vilnius (Lithuania); Duchovskis, P [Lithuanian Institute of Horticulture, Babtai, LT-54333 Kaunas District (Lithuania); Bliznikas, Z [Institute of Materials Science and Applied Research, Vilnius University, Sauletekio al. 9-III, LT-10222 Vilnius (Lithuania); Breive, K [Institute of Materials Science and Applied Research, Vilnius University, Sauletekio al. 9-III, LT-10222 Vilnius (Lithuania); Ulinskaite, R [Lithuanian Institute of Horticulture, Babtai, LT-54333 Kaunas District (Lithuania); Brazaityte, A [Lithuanian Institute of Horticulture, Babtai, LT-54333 Kaunas District (Lithuania); Novickovas, A [Institute of Materials Science and Applied Research, Vilnius University, Sauletekio al. 9-III, LT-10222 Vilnius (Lithuania); Zukauskas, A [Institute of Materials Science and Applied Research, Vilnius University, Sauletekio al. 9-III, LT-10222 Vilnius (Lithuania)

    2005-09-07

    Based on perspectives of the development of semiconductor materials systems for high-power light-emitting diodes (LEDs), an illumination facility for greenhouse plant cultivation was designed with the dominating 640 nm photosynthetically active component delivered by AlGaInP LEDs and supplementary components from AlGaN (photothropic action, 455 nm) and AlGaAs (photosynthetic 660 nm and photomorphogenetic 735 nm) LEDs. Photosynthesis intensity, photosynthetic productivity and growth morphology as well as chlorophyll and phytohormone concentrations were investigated in radish and lettuce grown in phytotron chambers under the LED-based illuminators and under high-pressure sodium (HPS) lamps with an equivalent photon flux density. Advantages of the high-power LED-based illuminators over conventional HPS lamps, applicability of AlGaInP LEDs for photosynthesis and control of plant growth by circadian manipulation of a relatively weak far-red component were demonstrated.

  20. High-power light-emitting diode based facility for plant cultivation

    International Nuclear Information System (INIS)

    Based on perspectives of the development of semiconductor materials systems for high-power light-emitting diodes (LEDs), an illumination facility for greenhouse plant cultivation was designed with the dominating 640 nm photosynthetically active component delivered by AlGaInP LEDs and supplementary components from AlGaN (photothropic action, 455 nm) and AlGaAs (photosynthetic 660 nm and photomorphogenetic 735 nm) LEDs. Photosynthesis intensity, photosynthetic productivity and growth morphology as well as chlorophyll and phytohormone concentrations were investigated in radish and lettuce grown in phytotron chambers under the LED-based illuminators and under high-pressure sodium (HPS) lamps with an equivalent photon flux density. Advantages of the high-power LED-based illuminators over conventional HPS lamps, applicability of AlGaInP LEDs for photosynthesis and control of plant growth by circadian manipulation of a relatively weak far-red component were demonstrated

  1. Comparison of phenotype of gammadelta T cells generated using various cultivation methods.

    Science.gov (United States)

    Mehrle, Stefan; Watzl, Carsten; von Lilienfeld-Toal, Marie; Amoroso, Alfredo; Schmidt, Jan; Märten, Angela

    2009-06-30

    It has been demonstrated, that gammadelta T cells play an important role in the development of immune responses to many pathogens. gammadelta T cells play a role in the clearance of viral and microbiological infections, anti-tumor responses, but also in autoimmune diseases. Many different protocols for the isolation and cultivation of gammadelta T cells can be found in the literature. Here we compare three common cultivation protocols for gammadelta T cells derived from peripheral blood with a newly developed protocol depending on SLAM (Signaling Lymphocyte Activation Molecule) stimulation. We demonstrate that the cultivation protocol chosen to raise gammadelta T cells has direct impact on the resulting gammadelta T cell phenotype. We show differences in gammadelta TCR composition, memory phenotype formation, CD8 receptor expression and the expression of NK cell markers depending on the stimulation protocol used. As such, the cultivation protocol chosen for a series of experiments might have significant impact on the outcome of the experiments and should be considered carefully.

  2. Cultivable endophytic bacteria from leaf bases of Agave tequilana and their role as plant growth promoters

    Science.gov (United States)

    Martínez-Rodríguez, Julia del C.; la Mora-Amutio, Marcela De; Plascencia-Correa, Luis A.; Audelo-Regalado, Esmeralda; Guardado, Francisco R.; Hernández-Sánchez, Elías; Peña-Ramírez, Yuri J.; Escalante, Adelfo; Beltrán-García, Miguel J.; Ogura, Tetsuya

    2014-01-01

    Agave tequilana Weber var. ‘Azul’ is grown for the production of tequila, inulin and syrup. Diverse bacteria inhabit plant tissues and play a crucial role for plant health and growth. In this study culturable endophytic bacteria were extracted from leaf bases of 100 healthy Agave tequilana plants. In plant tissue bacteria occurred at mean population densities of 3 million CFU/g of fresh plant tissue. Three hundred endophytic strains were isolated and 16s rDNA sequences grouped the bacteria into eight different taxa that shared high homology with other known sequences. Bacterial endophytes were identified as Acinectobacter sp., A. baumanii, A. bereziniae, Cronobacter sakazakii, Enterobacter hormaechei, Bacillus sp. Klebsiella oxytoca, Pseudomonas sp., Enterococcus casseliflavus, Leuconostoc mesenteroides subsp. mesenteroides and Gluconobacter oxydans. Isolates were confirmed to be plant growth promoting bacteria (PGPB) by their capacities for nitrogen fixation, auxin production, phosphate solubilization, or antagonism against Fusarium oxysporum AC132. E. casseliflavus JM47 and K. oxytoca JM26 secreted the highest concentrations of IAA. The endophyte Acinectobacter sp. JM58 exhibited the maximum values for nitrogen fixation and phosphate solubilization index (PSI). Inhibition of fungi was found in Pseudomonas sp. JM9p and K. oxytoca JM26. Bacterial endophytes show promise for use as bio-inoculants for agave cultivation. Use of endophytes to enhance cultivation of agave may be particularly important for plants produced by micropropagation techniques, where native endophytes may have been lost. PMID:25763038

  3. Cultivable endophytic bacteria from leaf bases of Agave tequilana and their role as plant growth promoters

    Directory of Open Access Journals (Sweden)

    Julia del C. Martínez-Rodríguez

    2014-12-01

    Full Text Available Agave tequilana Weber var. 'Azul' is grown for the production of tequila, inulin and syrup. Diverse bacteria inhabit plant tissues and play a crucial role for plant health and growth. In this study culturable endophytic bacteria were extracted from leaf bases of 100 healthy Agave tequilana plants. In plant tissue bacteria occurred at mean population densities of 3 million CFU/g of fresh plant tissue. Three hundred endophytic strains were isolated and 16s rDNA sequences grouped the bacteria into eight different taxa that shared high homology with other known sequences. Bacterial endophytes were identified as Acinectobacter sp., A. baumanii, A. bereziniae, Cronobacter sakazakii, Enterobacter hormaechei, Bacillus sp. Klebsiella oxytoca, Pseudomonas sp., Enterococcus casseliflavus, Leuconostoc mesenteroides subsp. mesenteroides and Gluconobacter oxydans. Isolates were confirmed to be plant growth promoting bacteria (PGPB by their capacities for nitrogen fixation, auxin production, phosphate solubilization, or antagonism against Fusarium oxysporum AC132. E. casseliflavus JM47 and K. oxytoca JM26 secreted the highest concentrations of IAA. The endophyte Acinectobacter sp. JM58 exhibited the maximum values for nitrogen fixation and phosphate solubilization index (PSI. Inhibition of fungi was found in Pseudomonas sp. JM9p and K. oxytoca JM26. Bacterial endophytes show promise for use as bio-inoculants for agave cultivation. Use of endophytes to enhance cultivation of agave may be particularly important for plants produced by micropropagation techniques, where native endophytes may have been lost.

  4. Carotenoid profiling from 27 types of paprika (Capsicum annuum L.) with different colors, shapes, and cultivation methods.

    Science.gov (United States)

    Kim, Ji-Sun; An, Chul Geon; Park, Jong-Suk; Lim, Yong Pyo; Kim, Suna

    2016-06-15

    In this study, we investigated carotenoid profiles and contents from 27 types of paprika with different colors (red, orange, and yellow), shapes (blocky and conical), and cultivation methods (soil and soilless). We simultaneously analyzed 12 kinds of carotenoids using UPLC equipped with an HSS T3 column for 30 min, and we identified six kinds of carotenoids in red paprika and nine types in orange and yellow paprika. Zeaxanthin concentrations in orange paprika were in the range of 85.06±23.37-151.39±5.94 mg/100 g dry weight (dw), which shows that orange paprika is a great source of zeaxanthin. Generally, red paprika is a great source of capsanthin. However, a new cultivar, 'Mini Goggal Red', contained large amounts of zeaxanthin (121.41±30.10 mg/100 g dw) even though its visible color is red. This is very meaningful considering that consumers have a preference for red color and the potent functional value of zeaxanthin. Carotenoid profiles and concentrations in blocky and conical type paprika were not significantly different in red paprika except the 'Mini Goggal Red' cultivar and yellow paprika. Blocky type orange paprika contains plenty of zeaxanthin, unlike conical type orange paprika. Three new cultivars of the conical type were cultivated in both soil culture and soilless culture in the same province, and carotenoid profiles and concentrations were similar, showing that both cultivations methods can be used. PMID:26868549

  5. Model-based design of protected cultivation system - first results and remaining challenges

    OpenAIRE

    Henten, van, J.W.; Vanthoor, B.H.E.; Stanghellini, C.; Visser, de, P.H.B.; Hemming, S.

    2012-01-01

    Abstract: Protected cultivation systems are used throughout the world as a powerful instrument to produce crops. They protect the crops from unfavorable outdoor climate conditions and pests and offer the opportunity to modify the indoor climate to create an environment that is optimal for crop growth and production, both in terms of quality and quantity. A quick scan of protected cultivation systems presently in use reveals that quite a variety of protected cultivation systems can be found th...

  6. Mechanized methods for harvesting residual biomass from Mediterranean fruit tree cultivations

    OpenAIRE

    Borja Velázquez-Martí; Elena Fernández-González; Ángel Jesús Callejón-Ferre; Javier Estornell-Cremades

    2012-01-01

    This study evaluates the technology and work systems used in order to harvest residual biomass from pruning in the specific conditions of Mediterranean fruit orchards (narrow distances between crop-rows). Harvesting has been divided into several types of operations - pruning, biomass alignment between crop tracks, biomass concentration in piles, chipping and bundling - which have been analyzed in five Mediterranean cultivations for three years. Altogether, three types of pruning have been ana...

  7. Comparison of Cheng's Index-and SSR Marker-based Classification of Asian Cultivated Rice

    Institute of Scientific and Technical Information of China (English)

    WANG Cai-hong; XU Qun; YU Ping; YUAN Xiao-ping; YU Han-yong; WANG Yi-ping; TANG Sheng-xiang

    2013-01-01

    A total of 100 cultivated rice accessions,with a clear isozyme-based classification,were analyzed based on Cheng's index and simple sequence repeat (SSR) marker.The results showed that the isozyme-based classification was in high accordance with that based on Cheng's index and SSR markers.Mantel-test revealed that the Euclidean distance of Cheng's index was significantly correlated with Nei's unbiased genetic distance of SSR markers (r =0.466,P ≤ 0.01).According to the model-based group and cluster analysis,the Cheng's index-and SSR-based classification coincided with each other,with the goodness of fit of 82.1% and 84.7% in indica,97.4% and 95.1% in japonica,respectively,showing higher accordance than that within subspecies.Therefore,Cheng's index could be used to classify subspecies,while SSR marker could be more efficient to analyze the subgroups within subspecies.

  8. The Production of High Purity Phycocyanin by Spirulina platensis Using Light-Emitting Diodes Based Two-Stage Cultivation.

    Science.gov (United States)

    Lee, Sang-Hyo; Lee, Ju Eun; Kim, Yoori; Lee, Seung-Yop

    2016-01-01

    Phycocyanin is a photosynthetic pigment found in photosynthetic cyanobacteria, cryptophytes, and red algae. In general, production of phycocyanin depends mainly on the light conditions during the cultivation period, and purification of phycocyanin requires expensive and complex procedures. In this study, we propose a new two-stage cultivation method to maximize the quantitative content and purity of phycocyanin obtained from Spirulina platensis using red and blue light-emitting diodes (LEDs) under different light intensities. In the first stage, Spirulina was cultured under a combination of red and blue LEDs to obtain the fast growth rate until reaching an absorbance of 1.4-1.6 at 680 nm. Next, blue LEDs were used to enhance the concentration and purity of the phycocyanin in Spirulina. Two weeks of the two-stage cultivation of Spirulina yielded 1.28 mg mL(-1) phycocyanin with the purity of 2.7 (OD620/OD280). PMID:26433600

  9. Method of cultivated land landscape ecological security evaluation and its application%耕地景观生态安全评价方法及其应用

    Institute of Scientific and Technical Information of China (English)

    裴欢; 魏勇; 王晓妍; 覃志豪; 侯春良

    2014-01-01

    Ecological security is very important for regional development and society stability, and it has become a hot topic currently. The concept of ecological security can be understood from two aspects, namely the broader sense and the narrower sense. In a broader sense, ecological security includes natural, economic, and social ecological security, while in a narrow sense, it means the security of natural and half natural ecological system, including ecological system and environment security. As special public resources, the quantity and quality of cultivated land are closely related to national food security and social stability, and has great significance in the maintenance of national security. In this study, taking the cultivated land in eastern low hills and coastal region of Qinhuangdao, China as the research area, a cultivated land landscape ecological security evaluation model was built based on the principles of landscape ecology and ecological security. The model contained five landscape pattern indexes including fragmentation, isolation, fractal dimension and shape factor, which could reflect production efficiency, stability and resilience of cultivated land. With three TM(ETM+) remote sensing images in 1990, 2000, and 2010 as well as GIS techniques, the landscape ecological security of cultivated land in the research area was comprehensively evaluated based on each town unit, and the evolution of landscape ecological security pattern and gravity center was analyzed using center of gravity method. At last, the driving factors of landscape ecological security evolution were discussed. The results showed that the landscape ecological security of cultivated land in the research area had been declining all the time with the value decreasing from 0.63 in 1993 to 0.5 in 2000. The fragmentation of cultivated land in low mountainous and hilly areas was more serious, so its landscape ecological security was lower than coastal area. The landscape ecological security

  10. Carbon and Nitrous Oxide Exchange from a Bioenergy Crop Cultivation on a Mineral Soil Measured with Eddy Covariance Method

    Science.gov (United States)

    Lind, S.; Shurpali, N.; Martikainen, P. J.

    2009-12-01

    Increased concentration of greenhouse gases (GHG; CO2, CH4, N2O) in the atmosphere have been linked to anthropogenic activities. Energy production based on fossil fuels is the main sources of CO2 to the atmosphere. Strategies to reduce CO2 emissions include use of bioenergy crops as energy source. To evaluate the real atmospheric value of bioenergy, knowledge on CO2 and N2O balances of ecosystems under bioenergy crop cultivation is needed. Reed canary grass (RCG, Phalaris arundinaceae L.) is increasingly being used as a bioenergy crop in the Nordic countries. It grows well in northern Europe as it tolerates flooding, requires short growing season and thrives well under low temperatures. Currently RCG is cultivated on an area of about 19000 ha in Finland and it is the highest yielding grass grown for energy purpose in Finland. The long rotation period (about ten years) of RCG cultivation without tilling favors carbon allocation into soil. First results from RCG cultivations on peat soil show that it is a promising land use option on such soils favoring soil carbon sequestration with low N2O emissions (Hyvönen et al. 2009; Shurpali et al. 2009). In order to devise a sound bioenergy policy based on this grass, studies on soils with variable characteristics under different climatic and hydrological regimes is urgently needed. Micrometeorological eddy covariance technique is suitable to study GHG fluxes at an ecosystem level (Baldocchi 2003). It is useful for continuous, direct and long-term measurements. Eddy covariance technique has been successfully used for measuring CO2 exchange in various ecosystems. The eddy covariance technique has also been used to measure N2O emissions (Denmead 2008). Aims of this study are to quantify the CO2 and N2O exchange of RCG cultivation on a mineral soil employing eddy covariance technique and to identify the key factors controlling the gas exchange. The study site on mineral soil is located in Eastern Finland. RCG was sown in spring

  11. Calculating and testing cultivated land protection externality using Contingent Valuation Method%基于CVM的耕地保护外部性估算与检验

    Institute of Scientific and Technical Information of China (English)

    牛海鹏; 王文龙; 张安录

    2014-01-01

    This paper proposed the Contingent Valuation Method (CVM) for estimating cultivated land protection externality. The proposed CVM was based on reconstruction of cultivated land utilization benefit system and defined cultivated land protection externality. The paper discussed in detail specific techniques of sample size and distribution, determination of leading technology, pay/compensation interval setting, hypothetical market scenario setting, evasion of deviation, pretreatment of survey data, and results validity/reliability tests. The results showed that ecological and social benefits of cultivated lands included public goods with mixed classes across categories. These classes were provided by joint ventures between the pure positive externalities and the private sector (mainly farmers). This could be estimated by using CVM and other non-market valuation methods. The willingness to pay of inter-viewee for this service was heavily restricted and influenced by their levels of socioeconomic development. The recognition of eco-logical and social benefits of cultivated lands of farmers and urban residents formed the main causality factor for the differences in payment ratio between urban residents and rural farmers. Urban residents were more familiar with ecological and social benefits of cultivated lands than rural/farm households. This was probably a key factor influencing the willingness to pay for ecological services. Farm households preferred voluntary labor as mean of payment, while both money and voluntary labor were more preferred by urban residents. The probable reason for this was that farm households had more leisure time than urban residents. Unitary cultivated land protection externality for Jiaozuo City was 1.91×108 Yuan·a−1 and the cultivated land protection externality per unit area of cultivated land was 993.7 Yuan·hm−2·a−1 base on CVM. CVM validity and reliability test was a critical element of CVM theory, method and practice. This was

  12. Improvement of erythromycin production by Saccharopolyspora erythraea in molasses based medium through cultivation medium optimization.

    Science.gov (United States)

    El-Enshasy, H A; Mohamed, N A; Farid, M A; El-Diwany, A I

    2008-07-01

    In the present work, erythromycin production was carried out in submerged culture using Saccharopolyspora erythraea. Different experiments were conducted to optimize the cultivation medium through the change of carbon and nitrogen sources to cheaper one in order to reduce the cost of medium and to utilize sugar cane molasses as one of major sugar industry by-products in Egypt. It was found that the addition of sugar cane molasses a sole carbon source at a concentration of 60 g/l accompanied by corn steep liquor (as organic N-source) in combination with ammonium sulphate (as inorganic N-source) gave the maximal erythromycin production. The antibiotic production in this medium reached about 600 mg/l which is about 33% higher than the value obtained in glucose based medium. On the other hand, the addition of n-propanol in concentration of 1% (v/v) increased the antibiotic production reaching about 720 mg/l after 144 h. Concluding, the new medium formulation based on cheap carbon source, sugar cane molasses, was a good alternative solution for the production of erythromycin economically. PMID:17936622

  13. Comparison of pooled standard deviation and standardized-t bootstrap methods for estimating uncertainty about average methane emission from rice cultivation

    Science.gov (United States)

    Kang, Namgoo; Jung, Min-Ho; Jeong, Hyun-Cheol; Lee, Yung-Seop

    2015-06-01

    The general sample standard deviation and the Monte-Carlo methods as an estimate of confidence interval is frequently being used for estimates of uncertainties with regard to greenhouse gas emission, based on the critical assumption that a given data set follows a normal (Gaussian) or statistically known probability distribution. However, uncertainty estimated using those methods are severely limited in practical applications where it is challenging to assume the probability distribution of a data set or where the real data distribution form appears to deviate significantly from statistically known probability distribution models. In order to solve these issues encountered especially in reasonable estimation of uncertainty about the average of greenhouse gas emission, we present two statistical methods, the pooled standard deviation method (PSDM) and the standardized-t bootstrap method (STBM) based upon statistical theories. We also report interesting results of the uncertainties about the average of a data set of methane (CH4) emission from rice cultivation under the four different irrigation conditions in Korea, measured by gas sampling and subsequent gas analysis. Results from the applications of the PSDM and the STBM to these rice cultivation methane emission data sets clearly demonstrate that the uncertainties estimated by the PSDM were significantly smaller than those by the STBM. We found that the PSDM needs to be adopted in many cases where a data probability distribution form appears to follow an assumed normal distribution with both spatial and temporal variations taken into account. However, the STBM is a more appropriate method widely applicable to practical situations where it is realistically impossible with the given data set to reasonably assume or determine a probability distribution model with a data set showing evidence of fairly asymmetric distribution but severely deviating from known probability distribution models.

  14. A SSR-based composite genetic linkage map for the cultivated peanut (Arachis hypogaea L. genome

    Directory of Open Access Journals (Sweden)

    Li Shaoxiong

    2010-01-01

    Full Text Available Abstract Background The construction of genetic linkage maps for cultivated peanut (Arachis hypogaea L. has and continues to be an important research goal to facilitate quantitative trait locus (QTL analysis and gene tagging for use in a marker-assisted selection in breeding. Even though a few maps have been developed, they were constructed using diploid or interspecific tetraploid populations. The most recently published intra-specific map was constructed from the cross of cultivated peanuts, in which only 135 simple sequence repeat (SSR markers were sparsely populated in 22 linkage groups. The more detailed linkage map with sufficient markers is necessary to be feasible for QTL identification and marker-assisted selection. The objective of this study was to construct a genetic linkage map of cultivated peanut using simple sequence repeat (SSR markers derived primarily from peanut genomic sequences, expressed sequence tags (ESTs, and by "data mining" sequences released in GenBank. Results Three recombinant inbred lines (RILs populations were constructed from three crosses with one common female parental line Yueyou 13, a high yielding Spanish market type. The four parents were screened with 1044 primer pairs designed to amplify SSRs and 901 primer pairs produced clear PCR products. Of the 901 primer pairs, 146, 124 and 64 primer pairs (markers were polymorphic in these populations, respectively, and used in genotyping these RIL populations. Individual linkage maps were constructed from each of the three populations and a composite map based on 93 common loci were created using JoinMap. The composite linkage maps consist of 22 composite linkage groups (LG with 175 SSR markers (including 47 SSRs on the published AA genome maps, representing the 20 chromosomes of A. hypogaea. The total composite map length is 885.4 cM, with an average marker density of 5.8 cM. Segregation distortion in the 3 populations was 23.0%, 13.5% and 7.8% of the markers

  15. Biodiversity of segetal weed community in continuous potato cultivated with metribuzin-based weed control

    OpenAIRE

    Pawlonka Zbigniew; Rymuza Katarzyna; Starczewski Krzysztof; Bombik Antoni

    2015-01-01

    The objective of the work reported here was to determine the relationship between herbicide rate and the biodiversity of weed communities in potato cultivated in continuous cropping. A seven-year field experiment was conducted to examine the effect of 4 metribuzin rates and an uncontrol on weed infestation in successive years of continuous potato cultivation. The following indices were calculated: the Shannon-Wiener and Simpson’s indices of species diversity and the Simpson’s index of dominat...

  16. A ddRAD Based Linkage Map of the Cultivated Strawberry, Fragaria xananassa.

    Directory of Open Access Journals (Sweden)

    Jahn Davik

    Full Text Available The cultivated strawberry (Fragaria ×ananassa Duch. is an allo-octoploid considered difficult to disentangle genetically due to its four relatively similar sub-genomic chromosome sets. This has been alleviated by the recent release of the strawberry IStraw90 whole genome genotyping array. However, array resolution relies on the genotypes used in the array construction and may be of limited general use. SNP detection based on reduced genomic sequencing approaches has the potential of providing better coverage in cases where the studied genotypes are only distantly related from the SNP array's construction foundation. Here we have used double digest restriction-associated DNA sequencing (ddRAD to identify SNPs in a 145 seedling F1 hybrid population raised from the cross between the cultivars Sonata (♀ and Babette (♂. A linkage map containing 907 markers which spanned 1,581.5 cM across 31 linkage groups representing the 28 chromosomes of the species. Comparing the physical span of the SNP markers with the F. vesca genome sequence, the linkage groups resolved covered 79% of the estimated 830 Mb of the F. × ananassa genome. Here, we have developed the first linkage map for F. × ananassa using ddRAD and show that this technique and other related techniques are useful tools for linkage map development and downstream genetic studies in the octoploid strawberry.

  17. A ddRAD Based Linkage Map of the Cultivated Strawberry, Fragaria xananassa.

    Science.gov (United States)

    Davik, Jahn; Sargent, Daniel James; Brurberg, May Bente; Lien, Sigbjørn; Kent, Matthew; Alsheikh, Muath

    2015-01-01

    The cultivated strawberry (Fragaria ×ananassa Duch.) is an allo-octoploid considered difficult to disentangle genetically due to its four relatively similar sub-genomic chromosome sets. This has been alleviated by the recent release of the strawberry IStraw90 whole genome genotyping array. However, array resolution relies on the genotypes used in the array construction and may be of limited general use. SNP detection based on reduced genomic sequencing approaches has the potential of providing better coverage in cases where the studied genotypes are only distantly related from the SNP array's construction foundation. Here we have used double digest restriction-associated DNA sequencing (ddRAD) to identify SNPs in a 145 seedling F1 hybrid population raised from the cross between the cultivars Sonata (♀) and Babette (♂). A linkage map containing 907 markers which spanned 1,581.5 cM across 31 linkage groups representing the 28 chromosomes of the species. Comparing the physical span of the SNP markers with the F. vesca genome sequence, the linkage groups resolved covered 79% of the estimated 830 Mb of the F. × ananassa genome. Here, we have developed the first linkage map for F. × ananassa using ddRAD and show that this technique and other related techniques are useful tools for linkage map development and downstream genetic studies in the octoploid strawberry.

  18. Ground-based studies of tropisms in hardware developed for the European Modular Cultivation System (EMCS)

    Science.gov (United States)

    Correll, Melanie J.; Edelmann, Richard E.; Hangarter, Roger P.; Mullen, Jack L.; Kiss, John Z.

    Phototropism and gravitropism play key roles in the oriented growth of roots in flowering plants. In blue or white light, roots exhibit negative phototropism, but red light induces positive phototropism in Arabidopsis roots. The blue-light response is controlled by the phototropins while the red-light response is mediated by the phytochrome family of photoreceptors. In order to better characterize root phototropism, we plan to perform experiments in microgravity so that this tropism can be more effectively studied without the interactions with the gravity response. Our experiments are to be performed on the European Modular Cultivation System (EMCS), which provides an incubator, lighting system, and high resolution video that are on a centrifuge palette. These experiments will be performed at μg, 1g (control) and fractional g-levels. In order to ensure success of this mission on the International Space Station, we have been conducting ground-based studies on growth, phototropism, and gravitropism in experimental unique equipment (EUE) that was designed for our experiments with Arabidopsis seedlings. Currently, the EMCS and our EUE are scheduled for launch on space shuttle mission STS-121. This project should provide insight into how the blue- and red-light signaling systems interact with each other and with the gravisensing system.

  19. The influence of cultivation method on the flowering of Salvia horminum L. 'Oxford Blue'

    Directory of Open Access Journals (Sweden)

    Elżbieta Pogroszewska

    2012-12-01

    Full Text Available Salvia horminum L. 'Oxford Blue' was grown from transplants or it was direct seeded. Four sowing dates were applied: 15 March or 30 March - in a greenhouse, in order to obtain transplants, 13 April, 27 April and 11 May - sown directly into the ground. Three plant densities were applied: 25 plants×m2, 16 plants×m2, 12 plants×m2. It was found that Horminum sage can be grown from transplants or direct seeded. The cultivation from transplants is more advantageous due to the earlier flowering of plants, by about two weeks, and a better quality of inflorescences evaluated in terms of their length and size of bracteoles. Direct sowing of Horminum sage at two-week intervals from the 2nd decade of April till the 2nd decade of May ensures that by the end of August mature inflorescence stems are obtained, ready for cutting. A delay in the date of sowing results in the development of significantly shorter inflorescence stems, irrespective of the plant density. Plant density does not affect significantly the length of the period of inflorescence formation and the date of flowering, but a larger spacing is favourable to plants growing big, what results in a larger fresh weight of the above-ground portion. Sage grown from transplants responses to favourable climatic conditions by the development of bigger inflorescences.

  20. Effects of site conditions and methods of cultivation on growth of sawtooth oak plantations

    Institute of Scientific and Technical Information of China (English)

    Luozhong TANG; Mukui YU; Dan ZHAO; Chunfeng YAN; Zhilong LIU; Shengzuo FANG

    2009-01-01

    The effects of site conditions and cultivation on the growth of sawtooth oak {Quercus acutissima Carr.) plantations were evaluated at the Hongyashan forest farm, in Chuzhou City, Anhui Province, China. The results indicate that the position on the slope, the amount of gravel and the thickness of the soil were important factors in the growth of the sawtooth oak. Lower slope positions with small amounts of gravel and a thick soil were better for the growth of this species than middle slope positions with more gravel and a thin soil. Given the site conditions of the hilly and mountainous areas in Chuzhou City, the mixed Chinese fir (Cunninghamia lanceolata Hook.) and sawtooth oak forests did not improve forest productivity compared with pure sawtooth oak forests. Both urea and compound fertilizers promoted the growth of sawtooth oak, as did site preparation and intercropping. Two years after planting, the height growth of ordinary seedlings with a starting height of 0.6 m was higher than that of supper seedlings with a starting height of 1.0 m. Compared with planting, the early growth of the coppices was faster, but the later growth of the coppices was slower.

  1. Mechanized methods for harvesting residual biomass from Mediterranean fruit tree cultivations

    Directory of Open Access Journals (Sweden)

    Borja Velázquez-Martí

    2012-06-01

    Full Text Available This study evaluates the technology and work systems used in order to harvest residual biomass from pruning in the specific conditions of Mediterranean fruit orchards (narrow distances between crop-rows. Harvesting has been divided into several types of operations - pruning, biomass alignment between crop tracks, biomass concentration in piles, chipping and bundling - which have been analyzed in five Mediterranean cultivations for three years. Altogether, three types of pruning have been analyzed: Manual, previous mechanical followed by manual, and fully mechanical; Two types of alignment: Manual and mechanical; Three concentration systems: Manual, tractor with a rake and a forwarder; Four chipping work organization systems: chipper driven inside orchard and manually fed by operators, mobile chipper driven inside orchard with pick-up header, mobile chipper fed by means of mechanical crane, chipper mounted on a truck fed by means of mechanical crane, which was working in a fixed position in a border of the plot after wood concentration. Also two bundling organization systems were checked: bundler machine working in a fixed position after wood concentration and working inside the plot driven among the crops. Previous concentration of the materials was the best alternative for their chipping or bundling in the studied conditions. Regression models have been calculated to predict the time of work of machinery and labor for each alternative. These equations were used to implement logistic planning as the Borvemar model, which defines a logistics network for supplying bio-energy systems.

  2. Production Process for Stem Cell Based Therapeutic Implants: Expansion of the Production Cell Line and Cultivation of Encapsulated Cells

    Science.gov (United States)

    Weber, C.; Pohl, S.; Poertner, R.; Pino-Grace, Pablo; Freimark, D.; Wallrapp, C.; Geigle, P.; Czermak, P.

    Cell based therapy promises the treatment of many diseases like diabetes mellitus, Parkinson disease or stroke. Microencapsulation of the cells protects them against host-vs-graft reactions and thus enables the usage of allogenic cell lines for the manufacturing of cell therapeutic implants. The production process of such implants consists mainly of the three steps expansion of the cells, encapsulation of the cells, and cultivation of the encapsulated cells in order to increase their vitality and thus quality. This chapter deals with the development of fixed-bed bioreactor-based cultivation procedures used in the first and third step of production. The bioreactor system for the expansion of the stem cell line (hMSC-TERT) is based on non-porous glass spheres, which support cell growth and harvesting with high yield and vitality. The cultivation process for the spherical cell based implants leads to an increase of vitality and additionally enables the application of a medium-based differentiation protocol.

  3. Research on Higher Vocational Student Professional Skill Quality Cultivation Methods%高职学生业务技能素质养成的方法研究

    Institute of Scientific and Technical Information of China (English)

    陈志强

    2015-01-01

    Aiming to the present situation of higher vocational student professional skill quality, it analyzes the research meaning of current higher vocational student features and its professional skill cultivation method. By the curriculum education cyber gamification, skill base training and professional skill manage-ment culture construction, it can work out the problems of higher vocational student profession skill quality cultivation.%针对高职学生的业务技能素质现况,分析目前高职学生的特点及其业务技能素质养成方法的研究意义;通过课程教育网络游戏化、技能基地训练和业务技能管理文化建设等途径,可以较好地解决高职学生业务技能素质养成的感知、熟知和精准问题。

  4. Cultivation and utilization of specific wood biomass for synthesis of cellulose based bioethanol

    Energy Technology Data Exchange (ETDEWEB)

    Fara, L.; Comaneci, D. [Polytechnic Univ. of Bucharest, Bucharest (Romania). Faculty of Applied Sciences; Cincu, C.; Hubca, G.; Zaharia, C.; Diacon, A. [Polytechnic Univ. of Bucharest, Bucharest (Romania). Faculty of Applied Chemistry; Filat, M.; Chira, D. [Forest Research and Management Inst., Ilfov (Romania); Nutescu, C. [National Wood Inst., Bucharest (Romania); Fara, S. [Inst. for Research and Design of Automation, Bucharest (Romania)

    2010-07-01

    The energetic characteristics of 6 types of poplar clones cultivated for different pedoclimatic conditions in Romania were determined. Four clones were developed in Italy and 2 in Romania. Five experimental cultures were used to analyze the plant survival rate and biomass production rate. After 2 years of study, the Italian clones were found to have very good adaptability to the pedoclimatic conditions in Romania in comparison with local clones. The Italian clones Monviso and AF-6 registered the most substantial growths and the highest resistance to disease. Bioethanol was synthesized by acidic hydrolysis of the cellulose using 2 approaches. In the first approach the lignocellulosic raw material was hydrolyzed with diluted sulfuric acid at 50 degrees C for 24 hours. After filtration, the solid residue was treated with 30 per cent H{sub 2}SO{sub 4} at 100 degrees C for 6 hours. The resulting solutions were neutralized with Ca(OH){sub 2} following another filtration and the resulted solution with pH 6.5 was subjected to fermentation with Saccharomices Cerevisiae. In the second approach the lignocellulosic raw material was subjected to hydrolysis with 10 per cent H{sub 2}SO{sub 4} at 100 degrees C for 4 hours. After filtration, the solid residue was hydrolyzed with 30 per cent H{sub 2}SO{sub 4} at 100 degrees for 6 hours. The solution was neutralized with Ca(OH){sub 2} and subjected to alcoholic fermentation with Saccharomices Cerevisiae. The fermentation took place at 25 degrees C for 72 hours. The results for the two methods were similar.

  5. The Effect of Cultivated Wild Ginseng Extract on Preadipocyte Proliferation

    Directory of Open Access Journals (Sweden)

    Byoung-Woo Kim

    2007-12-01

    Full Text Available Objectives : The purpose of this study is to investigate the effects of cultivated wild ginseng extract on primary cultured preadipocyte and adipocytes. Methods : Diminish preadipocyte proliferation does primary role to reduce obesity. So, preadipocytes and adipocytes were performed on cell cultures with using Sprague-Dawley rats and treated with 0.01-1mg/㎖ cultivated wild ginseng extract. Result : At all concentrations, cultivated wild ginseng extract wasn't show the suppress proliferation of preadipocytes significantly and failed to show effects on decomposition of adipocytes except high dosage. Conclusion : Based on these findings, cultivated wild ginseng is not a suitable choice for the treatment of localized obesity.

  6. Model based optimization of high cell density cultivation of nitrogen-fixing cyanobacteria.

    Science.gov (United States)

    Alagesan, Swathi; Gaudana, Sandeep B; Krishnakumar, S; Wangikar, Pramod P

    2013-11-01

    In the present study, fed-batch cultivation of Cyanothece sp. ATCC 51142, a known hydrogen producer, was optimized for maximizing biomass production. Decline in growth of this organism in dense cultures was attributed to increased substrate consumption for maintenance and respiration, and photolimitation due to self shading. A model incorporating these aspects was developed, and by using control vector parameterization (CVP), substrate feeding recipe was optimized to achieve 12-fold higher biomass concentration. The optimization results were verified experimentally on shake flask and bioreactor. The latter resulted in greater exponential growth rate possibly by overcoming photolimitation by simulating flashing light effect. Such a strategy can be readily applied for mixotrophic cultivation of cyanobacterial cultures in the first stage followed by photoautotrophic growth at the production stage. PMID:24047683

  7. Self-Organizing Map and Multi-Layer Perceptron Neural Network Based Data Mining To Envisage Agriculture Cultivation

    Directory of Open Access Journals (Sweden)

    E. T. Venkatesh

    2008-01-01

    Full Text Available Study on characteristics of soil, to determine the types of crops suitable for cultivation in a particular region can increase the yield to greater extent, which minimizes the expenditures involved in irrigation and application of fertilizers. With the tested techniques available for calibrating the quality of soil and the crops suitable for cultivation in it, it is possible to determine the exact crop, irrigation patterns and even the cycle and quantity of fertilizer application. This paper dealt with the application of SOM based clustering and Artificial Intelligence techniques, to analyze the patterns of soils distributed across huge geographical area and identify the suitable types of crops for the particular soil. Estimation of exact crop(s suitable for a particular region can help stave off redundant maintenance and the inherent expenditures that would occur due to over irrigation and over usage of fertilizers, to fulfill the natural deficiencies. Our Focus is to improve the optimal utilization of innate characteristics in a soil through cultivation of appropriate crops, which will increase the volume and quality of yield, in particular for a developing country like India, where the huge majority of the population depends primarily on agriculture for livelihood.

  8. Cultivating New-type Farmers Based on the Theory of Human Resources Development

    OpenAIRE

    Zhang, Li

    2010-01-01

    Under the direction of theory of human resources development, this thesis analyzes the impact of rural human resources development on cultivating new-type farmers. Firstly, it increases the input of rural basic education; secondly, it reinforces the vocational education and technology training; thirdly, it promotes the rural medical and public health services; fourthly, it quickens the rural labor transfer. The status quo of China’s rural human resources has been analyzed as follows: in ter...

  9. The Cultivation of Cluster’s Sustainable Competence Based on Knowledge Management

    OpenAIRE

    Tong Yang; Nengmin Wang

    2009-01-01

    The cluster has shown its powerful competitive advantages in global competition. However, as amounts of industrial clusters have displayed their competence, some have lost their competitive advantages in global competition. It is meaningful for clusters’ development to cultivate their sustainable competence. This paper analyzes the sources of cluster competence. According this paper, the important ways to make cluster competence sustainable include transferring and sharing knowledge, making i...

  10. Study of Cultivating Intercultural Awareness in High School--Based on English Euphemism

    Institute of Scientific and Technical Information of China (English)

    孔曼羚

    2014-01-01

    Euphemism, which is one of universal language phenomena, provides an important approach to improve the commu-nicative effects and maintain interpersonal relationship harmoniously. This paper aims to arouse the attention of high school Eng-lish teachers to cultivate students’intercultural awareness. This thesis, with a brief introduction of English euphemism, presents a profound study of how to promote the intercultural awareness of students in high school with the help of English euphemism.

  11. Research on the cultivation path of smart home-based care service mode in Internet+ vision

    Directory of Open Access Journals (Sweden)

    Peng Qingchao

    2016-01-01

    Full Text Available Home-based care for the aged is an effective method to solve the problem of caring the aged in China. This thesis analyzes some problems existing in the development of current home-based care service for the aged in our country and the positive effects brought by Internet+ in home-based care service. It proposes a new service mode of care for the aged--Internet+ home-based care service, and explains the establishment of this system and the responsibilities of the participants. Also, it explores the path to realize the establishment of Internet+ home-based care service mode so as to promote the healthy development of home-based care service in China.

  12. Characteristics of Eastern Canadian cultivated Sphagnum and potential use as a substitute for perlite and vermiculite in peat-based horticultural substrates

    Directory of Open Access Journals (Sweden)

    M. Aubé

    2015-03-01

    Full Text Available Sphagnum cultivation on harvested peatlands to meet wetland restoration objectives could be an economically feasible activity since cultivated Sphagnum has potential horticultural applications. We compared the characteristics of cultivated Sphagnum from Shippagan (Canada with those of non-cultivated Sphagnum products from Chile, New Zealand and Canada, and assessed its potential as a perlite and vermiculite substitute in horticultural peat-based substrates. Shippagan cultivated Sphagnum was shorter than the Chilean and New Zealand products with which it was compared, yet more similar to them than to the Canadian product currently on the market. Laboratory tests on physical properties and greenhouse growth trials indicated that 50–100 % of the perlite or vermiculite of a peat-based substrate can be successfully replaced with cultivated Sphagnum. Non-sieved coarsely shredded Sphagnum or the large (> 6.3 mm fragments of sieved coarsely shredded Sphagnum best replicated the aeration provided by perlite and vermiculite in the substrates that were tested. Decomposition tests and comparisons of changes in physical properties of substrates containing Sphagnum after six weeks of growth trials indicated that Sphagnum degradation leading to reduced substrate performance is not likely to be an issue. Therefore, cultivated Sphagnum has great potential as a substitute for perlite and vermiculite.

  13. Analysis of 2-(2-Phenylethyl)chromones by UPLC-ESI-QTOF-MS and Multivariate Statistical Methods in Wild and Cultivated Agarwood

    Science.gov (United States)

    Li, Yuanbin; Sheng, Nan; Wang, Lingli; Li, Shijie; Chen, Jiannan; Lai, Xiaoping

    2016-01-01

    Agarwood is the fragrant resinous material mainly formed from species of Aquilaria. 2-(2-phenylethyl)chromones, especially the highly oxidized 5,6,7,8-tetrahydro-2-(2-phenylethyl)chromones, are the main representative compounds from agarwood. It is important to determine whether agarwood in trade is from cultivated trees or natural trees in the Convention on the International Trade in Endangered Species (CITES). We characterized the 2-(2-phenylethyl)chromones in agarwood by ultra-performance liquid chromatography coupled with electrospray ionization mass spectrometry (UPLC–ESI-QTOF-MS) and differentiated wild from cultivated agarwood by metabolomic analysis. A total of 141 chromones including 50 potentially new compounds were evaluated as belonging to four structural classes (unoxidized 2-(2-phenylethyl)chromones, 5,6,7,8-tetrahydro-2-(2-phenylethyl)-chromones, bi-2-(2-phenylethyl)chromones, and tri-2-(2-phenylethyl)chromones). The metabolic difference between wild and cultivated agarwood was analyzed by component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). Fourteen markers of metabolisms in wild and cultivated agarwood were constructed (e.g., 6,7-dimethoxy-2-(2-phenylethyl)chromone, 6,8-dihydroxy-2-(2-phenylethyl)chromone, 6-methoxy-2-(2-phenylethyl)chromone, etc.). These results indicated that UPLC–ESI-QTOF-MS-based metabonomics analysis in agarwood may be useful for distinguishing wild agarwood from cultivated agarwood. PMID:27223280

  14. Analysis of 2-(2-Phenylethyl)chromones by UPLC-ESI-QTOF-MS and Multivariate Statistical Methods in Wild and Cultivated Agarwood.

    Science.gov (United States)

    Li, Yuanbin; Sheng, Nan; Wang, Lingli; Li, Shijie; Chen, Jiannan; Lai, Xiaoping

    2016-01-01

    Agarwood is the fragrant resinous material mainly formed from species of Aquilaria. 2-(2-phenylethyl)chromones, especially the highly oxidized 5,6,7,8-tetrahydro-2-(2-phenylethyl)chromones, are the main representative compounds from agarwood. It is important to determine whether agarwood in trade is from cultivated trees or natural trees in the Convention on the International Trade in Endangered Species (CITES). We characterized the 2-(2-phenylethyl)chromones in agarwood by ultra-performance liquid chromatography coupled with electrospray ionization mass spectrometry (UPLC-ESI-QTOF-MS) and differentiated wild from cultivated agarwood by metabolomic analysis. A total of 141 chromones including 50 potentially new compounds were evaluated as belonging to four structural classes (unoxidized 2-(2-phenylethyl)chromones, 5,6,7,8-tetrahydro-2-(2-phenylethyl)-chromones, bi-2-(2-phenylethyl)chromones, and tri-2-(2-phenylethyl)chromones). The metabolic difference between wild and cultivated agarwood was analyzed by component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). Fourteen markers of metabolisms in wild and cultivated agarwood were constructed (e.g., 6,7-dimethoxy-2-(2-phenylethyl)chromone, 6,8-dihydroxy-2-(2-phenylethyl)chromone, 6-methoxy-2-(2-phenylethyl)chromone, etc.). These results indicated that UPLC-ESI-QTOF-MS-based metabonomics analysis in agarwood may be useful for distinguishing wild agarwood from cultivated agarwood. PMID:27223280

  15. A one-stage cultivation process for lipid- and carbohydrate-rich biomass of Scenedesmus obtusiusculus based on artificial and natural water sources.

    Science.gov (United States)

    Schulze, Christian; Reinhardt, Jakob; Wurster, Martina; Ortiz-Tena, José Guillermo; Sieber, Volker; Mundt, Sabine

    2016-10-01

    A one-stage cultivation process of the microalgae Scenedesmus obtusiusculus with medium based on natural water sources was developed to enhance lipids and carbohydrates. A medium based on artificial sea water, Baltic Sea water and river water with optimized nutrient concentrations compared to the standard BG-11 for nitrate (-75%), phosphate and iron (-90%) was used for cultivation. Although nitrate exhaustion over cultivation resulted in nitrate limitation, growth of the microalgae was not reduced. The lipid content increased from 6.0% to 19.9%, an increase in oleic and stearic acid was observed. The unsaponifiable matter of the lipid fraction was reduced from 19.5% to 11.4%. The carbohydrate yield rose from 45% to 50% and the protein content decreased from 32.4% to 15.9%. Using natural water sources with optimized nutrient concentrations could open the opportunity to modulate biomass composition and to reduce the cultivation costs. PMID:27394996

  16. GIS-based characterization of the geographic distributions of wild and cultivated populations of the Mesoamerican fruit tree Spondias purpurea (Anacardiaceae).

    Science.gov (United States)

    Miller, Allison J; Knouft, Jason H

    2006-12-01

    Humans are having a profound impact on the geographic distributions of plant populations. In crop species, domestication has been accompanied by the geographic expansion of cultivated populations relative to their wild ancestors. We used a geographical information system (GIS)-based approach to investigate differences in the environmental factors characterizing the geographic distributions of cultivated and wild populations of the Mesoamerican fruit tree Spondias purpurea. Locality data for 86 cultivated and 28 wild S. purpurea populations were used in conjunction with environmental data layers and Maxent, a maximum entropy application for predicting species distributions. Interpredictivity analyses and principal components analysis revealed that the predicted distribution of wild S. purpurea is nested within the cultivated distribution and that the ecological niche (defined by environmental characteristics) of cultivated S. purpurea has expanded relative to that of wild populations. Significant differences between wild and cultivated populations were detected for five environmental variables, corresponding to the expansion of S. purpurea during the domestication process from its native habitat in the Mesoamerican tropical dry forests into less seasonal habitats. These data suggest that humans have altered the range of habitats occupied by cultivated S. purpurea populations relative to their wild progenitors.

  17. Methods in Logic Based Control

    DEFF Research Database (Denmark)

    Christensen, Georg Kronborg

    1999-01-01

    Desing and theory of Logic Based Control systems.Boolean Algebra, Karnaugh Map, Quine McClusky's algorithm. Sequential control design. Logic Based Control Method, Cascade Control Method. Implementation techniques: relay, pneumatic, TTL/CMOS,PAL and PLC- and Soft_PLC implementation. PLC...

  18. Activity based costing (ABC Method

    Directory of Open Access Journals (Sweden)

    Prof. Ph.D. Saveta Tudorache

    2008-05-01

    Full Text Available In the present paper the need and advantages are presented of using the Activity BasedCosting method, need arising from the need of solving the information pertinence issue. This issue has occurreddue to the limitation of classic methods in this field, limitation also reflected by the disadvantages ofsuch classic methods in establishing complete costs.

  19. A model based method for evaluation of crop operation scenarios in greenhouses

    NARCIS (Netherlands)

    Ooster, van 't A.

    2015-01-01

    Abstract This research initiated a model-based method to analyse labour in crop production systems and to quantify effects of system changes in order to contribute to effective greenhouse crop cultivation systems with efficient use of human labour and technology. This method was gra

  20. Estimation of the stand ages of tropical secondary forests after shifting cultivation based on the combination of WorldView-2 and time-series Landsat images

    Science.gov (United States)

    Fujiki, Shogoro; Okada, Kei-ichi; Nishio, Shogo; Kitayama, Kanehiro

    2016-09-01

    We developed a new method to estimate stand ages of secondary vegetation in the Bornean montane zone, where local people conduct traditional shifting cultivation and protected areas are surrounded by patches of recovering secondary vegetation of various ages. Identifying stand ages at the landscape level is critical to improve conservation policies. We combined a high-resolution satellite image (WorldView-2) with time-series Landsat images. We extracted stand ages (the time elapsed since the most recent slash and burn) from a change-detection analysis with Landsat time-series images and superimposed the derived stand ages on the segments classified by object-based image analysis using WorldView-2. We regarded stand ages as a response variable, and object-based metrics as independent variables, to develop regression models that explain stand ages. Subsequently, we classified the vegetation of the target area into six age units and one rubber plantation unit (1-3 yr, 3-5 yr, 5-7 yr, 7-30 yr, 30-50 yr, >50 yr and 'rubber plantation') using regression models and linear discriminant analyses. Validation demonstrated an accuracy of 84.3%. Our approach is particularly effective in classifying highly dynamic pioneer vegetation younger than 7 years into 2-yr intervals, suggesting that rapid changes in vegetation canopies can be detected with high accuracy. The combination of a spectral time-series analysis and object-based metrics based on high-resolution imagery enabled the classification of dynamic vegetation under intensive shifting cultivation and yielded an informative land cover map based on stand ages.

  1. Evaluation on Fuzzy Variable Weight of Cultivated Land Fertility Based on GIS

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    [Objective] The modified variable weights based on constant weight and in- troduced theory of equalization function would better incorporate authentic index weights and make evaluation results of fertility more scientific. [Method] In Gaozhou City, the final weights of influential factors can be determined with the help of GIS and as per AHP and theory of variable weights. In addition, farmland fertility was e- valuated in an automatic and quantitative way and the spatial distribution pattern was analyzed as per fuzzy comprehensive evaluation. [Result] For farmlands at 58 505.027 8 hm2 in the city, farmlands from grade 1 to grade 8 account for 3.62%, 18.27%, 33.15%, 26.96%, 13.66%, 3.29%, 0.81% and 0.24%, respectively, which is in consistent with local condition. [Conclusion] These results have been applied di- rectly in test regions and constitute a rewarding exploration for fertility evaluation in South China.

  2. 基于脱钩指数的2020年江苏省耕地保有量目标分析%Target Analysis of Cultivated Land Protection Area in Jiangsu Province in 2020 Based on Decoupling Index

    Institute of Scientific and Technical Information of China (English)

    肖丽群; 吴群

    2012-01-01

    脱钩理论是近年来国际上在测度经济增长与物质消耗之间关系过程中提出的一种理论方法。本研究引入脱钩理论中的脱钩指数及其评价标准,采用历史分析与未来预测相结合的方法,估算了2020年江苏省耕地保有量目标实现的难易程度及其耕地保有量缺口。并且利用试算内插法,测算出了实现耕地保有量目标时的脱钩指数值。研究结果表明:①近30年来,江苏省经济增长与耕地消耗的脱钩指数一直呈现波动下降的趋势,主要是结构效应和政策效应共同作用的结果;②实现2020年耕地保有量目标压力巨大,脱钩指数只有维持在不大于0.0012的水平,才有可能实现耕地保有量目标。因此,在保证经济增长的同时实现耕地保有量规划目标,则必须转变经济增长方式,采取更多积极的政策措施。%Rapid growth of economy inevitably requires a large quantity of land resources. In the next 10 years, China’s economy will enjoy rapid development, so limited cultivated land resources will be faced with great pressure. At the same time, economic growth will also be the main cause of cultivated land consumption. Based on the domestic and foreign researches, this paper has analyzed the relationship between economic growth and cultivated land consumption and estimated the difficulty of achieving the cultivated land protection target. Decoupling theory is a theoretical approach put forward in the process of measuring the relationship between economic growth and substance consumption by international researchers in recent years. The word"decoupling"is derived from physics. Agricultural trade negotiators, economists and policymakers use "decoupling"to interpret the departure relationship between agricultural trade and policy measures. This paper has estimated the difficulty of achieving the target of cultivated land protection area and its gap in Jiangsu Province in 2020 by introducing

  3. Comparative Analysis and Cultivation of New Agricultural Business Entities Based on Comparison of Family Farms and Professional Cooperatives

    Institute of Scientific and Technical Information of China (English)

    Fang; YANG; Xingxi; LIU; Yingliang; ZHANG

    2014-01-01

    Cultivating new bussiness entities is the key to speeding up improving new agricultural production and operating system.This study discussed two representative entities under the agricultural production and operating system based on household contract management,namely,family farms and cooperatives.It introduced current development status of these two entities in Chongqing region,and discussed prominent problems of understanding,management,fund,personnel,technology,information,and system in the development process.In line with common problems such as single production structure and short supply of agricultural insurance,and different problems like organizational degree,it made comparative analysis.Finally,it is concluded that different institutional arrangement and pertinent support policy according to different development characteristics.

  4. Fungal cultivation on glass-beads

    DEFF Research Database (Denmark)

    Droce, Aida; Sørensen, Jens Laurids; Giese, Henriette;

    Transcription of various bioactive compounds and enzymes are dependent on fungal cultivation method. In this study we cultivate Fusarium graminearum and Fusarium solani on glass-beads with liquid media in petri dishes as an easy and inexpensive cultivation method, that resembles in secondary meta...... metabolite production to agar-cultivation but with an easier and more pure RNA-extraction of total fungal mycelia....

  5. An Innovative Research on the Cultivation Pattern of Human Resources Management Professionals

    OpenAIRE

    Xia Cui; Yang Lu

    2009-01-01

    With the approaching era of knowlege-based economy as well as the gradual universalization of China’s higher education, the quantity issue in talent cultivation has given way to the quality one consequently. Setting out from some problems in the cultivation of human resources management professional in China, this article puts forward patterns for objectives, curriculum systems as well as teaching methods in innovative talent cultivation.

  6. Cultivating New-type Farmers Based on the Theory of Human Resources Development

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Under the direction of theory of human resources development,this thesis analyzes the impact of rural human resources development oncultivating new-type farmers.Firstly,it increases the input of rural basic education;secondly,it reinforces the vocational education and technologytraining;thirdly,it promotes the rural medical and public health services;fourthly,it quickens the rural labor transfer.The status quo of China’s ruralhuman resources has been analyzed as follows:in terms of the quantity of rural human resources,the status quo is large and quick-developed baseof rural human resources,high labor participatory rate,and constitution of low age;in terms of the quality of rural human resources,the status quois the ubiquitous low quality of rural human resources,low technological level of rural human resources,and overall low physical quality of farmers;in terms of the structure of rural human resources,the status quo is the irrational industrial structure distribution and imbalanced regional structuredistribution.The thesis also discusses the edification of theory of human resources development in cultivating new-type farmers.First,in terms ofthe control over quantity of rural human resources,it is to keep the stability of family planning policy,and expedite the transfer of rural surplus la-bor;second,in terms of promoting the quality of rural human resources,it is to bolster the development of reserve rural labor force resources,toconstruct the adult educational training system with Chinese characteristics,and to build rural primary health care system;third,in terms of adjus-ting the structure of rural human resources,it is to perfect rural human resources market,and adjust rural economical structure and talents struc-ture.

  7. Method for gesture based modeling

    DEFF Research Database (Denmark)

    2006-01-01

    A computer program based method is described for creating models using gestures. On an input device, such as an electronic whiteboard, a user draws a gesture which is recognized by a computer program and interpreted relative to a predetermined meta-model. Based on the interpretation, an algorithm...... is assigned to the gesture drawn by the user. The executed algorithm may, for example, consist in creating a new model element, modifying an existing model element, or deleting an existing model element....

  8. Gas chromatography/mass spectrometry-based metabolic profiling and differentiation of ginseng roots according to cultivation age using variable selection.

    Science.gov (United States)

    Park, Hae Eun; Lee, Seok-Young; Hyun, Sun-Hee; Kim, Da Yeon; Marriott, Philip J; Choi, Hyung-Kyoon

    2013-01-01

    Ginseng roots are an important herbal resource worldwide, and the adulteration of ginseng with age is recognized as a serious problem. It is therefore crucial to develop objective criteria or standard protocols for differentiating ginseng root samples according to their cultivation age. The reported study used GC/MS combined with multivariate statistical analysis with variable selection to obtain metabolic profiling and an optimal partial least squares-discriminant analysis (PLS-DA) model for the differentiation of ginseng according to cultivation age. Relative levels of various metabolites, such as amino acids, alcohols, fatty acids, organic acids, and sugars, were measured for various ginseng cultivation ages. Increasing cultivation age resulted in the production of higher levels of panaxynol and panaxydol, which are active polyacetylene compounds in ginseng. In addition, optimized PLS-DA models for the prediction of ginseng age were obtained by selecting variables based on a variable importance in the projection cut-off value of 1.3. Proline, glucaric acid, mannose, gluconic acid, glucuronic acid, myoinositol, panaxydol, and panaxynol are suggested as key and relevant compounds with which to differentiate the age of ginseng samples. The findings of this study suggest that GC/MS-based metabolic profiling can be used to differentiate ginseng samples according to cultivation age.

  9. Single-base resolution maps of cultivated and wild rice methylomes and regulatory roles of DNA methylation in plant gene expression

    Directory of Open Access Journals (Sweden)

    Li Xin

    2012-07-01

    Full Text Available Abstract Background DNA methylation plays important biological roles in plants and animals. To examine the rice genomic methylation landscape and assess its functional significance, we generated single-base resolution DNA methylome maps for Asian cultivated rice Oryza sativa ssp. japonica, indica and their wild relatives, Oryza rufipogon and Oryza nivara. Results The overall methylation level of rice genomes is four times higher than that of Arabidopsis. Consistent with the results reported for Arabidopsis, methylation in promoters represses gene expression while gene-body methylation generally appears to be positively associated with gene expression. Interestingly, we discovered that methylation in gene transcriptional termination regions (TTRs can significantly repress gene expression, and the effect is even stronger than that of promoter methylation. Through integrated analysis of genomic, DNA methylomic and transcriptomic differences between cultivated and wild rice, we found that primary DNA sequence divergence is the major determinant of methylational differences at the whole genome level, but DNA methylational difference alone can only account for limited gene expression variation between the cultivated and wild rice. Furthermore, we identified a number of genes with significant difference in methylation level between the wild and cultivated rice. Conclusions The single-base resolution methylomes of rice obtained in this study have not only broadened our understanding of the mechanism and function of DNA methylation in plant genomes, but also provided valuable data for future studies of rice epigenetics and the epigenetic differentiation between wild and cultivated rice.

  10. Effect of cultivation method on the number and quality of young Santolina chamaecyparissus L. plants after wintering

    Directory of Open Access Journals (Sweden)

    Zdzisław Guzikowski

    2013-12-01

    Full Text Available The growth of young Santolina chamaecyparissus plants in a non-heated foil tunnel was rated depending on term of rooting cuttings and type of growing container in three cycles of study: 2005/06, 2006/07, 2007/08. Young plants obtained from cuttings rooted on 16th of August and 16th of September were planted on 16th of November in various containers and grown till 8th of May of the following year. Multipot pallets, cellulose pots and plastic pots were used for cultivation. In May, after 8-9 months the number of plants and their quality were defined after wintering. Type of containers and age of young plants did not have a significant effect on the wintering. The best quality of plants – high and with a large number of shoots – was obtained from cultivation in plastic pots.

  11. On the Cultivation of Computing Thinking Ability in Nu-merical Computing Method Course%数值计算方法课程中计算思维能力的培养

    Institute of Scientific and Technical Information of China (English)

    胡金蓉; 吴霞

    2015-01-01

    With the continuous development of computer technol-ogy, computing thinking ability has become a necessary quality that college students must possess. The inherent characteristics of knowledge structure of numerical computing method, as a foun-dation course for science and engineering students, are of great significance to the cultivation of computing thinking. With the course as the breakthrough point, under the guidance of the out-line of cultivating computing thinking ability, this paper discusses the teaching scheme of numerical computing method course based on the cultivation of computing thinking ability, aiming to train and improve students' computing thinking ability based on their mastery of knowledge on numerical computing method.%随着计算机技术的不断发展,计算思维能力已成为大学生必须具备的基本素质之一。数值计算方法作为理工科学生的专业基础课程之一,其固有的知识结构特点对计算思维的培养具有重要意义。本文以该课程作为切入点,在计算思维能力培养概要指导下讨论了基于计算思维能力培养的数值计算方法课程的教学方案,目的是在学生学习数值计算方法内容的同时,训练和提升自己的计算思维能力。

  12. Population-based resequencing analysis of wild and cultivated barley revealed weak domestication signal of selection and bottleneck in the Rrs2 scald resistance gene region.

    Science.gov (United States)

    Fu, Yong-Bi

    2012-02-01

    Many plant disease resistance (R) genes have been cloned, but the potential of utilizing these plant R-gene genomic resources for genetic inferences of plant domestication history remains unexplored. A population-based resequencing analysis of the genomic region near the Rrs2 scald resistance gene was made in 51 accessions of wild and cultivated barley from 41 countries. Fifteen primer pairs were designed to sample the genomic region with a total length of 10 406 bp. More nucleotide diversity was found in wild (π = 0.01846) than cultivated (π = 0.01507) barley samples. Three distinct groups of 29 haplotypes were detected for all 51 samples, and they were well mixed with wild and cultivated barley samples from different countries and regions. The neutrality tests by Tajima's D were not significant, but a significant (P domestication. Together, the domestication signal in the genomic region was weak both in human selection and domestication bottleneck.

  13. A Spiral Step-by-Step Educational Method for Cultivating Competent Embedded System Engineers to Meet Industry Demands

    Science.gov (United States)

    Jing,Lei; Cheng, Zixue; Wang, Junbo; Zhou, Yinghui

    2011-01-01

    Embedded system technologies are undergoing dramatic change. Competent embedded system engineers are becoming a scarce resource in the industry. Given this, universities should revise their specialist education to meet industry demands. In this paper, a spirally tight-coupled step-by-step educational method, based on an analysis of industry…

  14. Single-base resolution maps of cultivated and wild rice methylomes and regulatory roles of DNA methylation in plant gene expression

    DEFF Research Database (Denmark)

    Li, Xin; Zhu, Jingde; Hu, Fengyi;

    2012-01-01

    DNA methylation plays important biological roles in plants and animals. To examine the rice genomic methylation landscape and assess its functional significance, we generated single-base resolution DNA methylome maps for Asian cultivated rice Oryza sativa ssp. japonica, indica and their wild...

  15. Classification method based on KCCA

    Science.gov (United States)

    Wang, Zhanqing; Zhang, Guilin; Zhao, Guangzhou

    2007-11-01

    Nonlinear CCA extends the linear CCA in that it operates in the kernel space and thus implies the nonlinear combinations in the original space. This paper presents a classification method based on the kernel canonical correlation analysis (KCCA). We introduce the probabilistic label vectors (PLV) for a give pattern which extend the conventional concept of class label, and investigate the correlation between feature variables and PLV variables. A PLV predictor is presented based on KCCA, and then classification is performed on the predicted PLV. We formulate a frame for classification by integrating class information through PLV. Experimental results on Iris data set classification and facial expression recognition show the efficiencies of the proposed method.

  16. Study on Compensation Standard of Land Expropriation Based on Comprehensive Value of Cultivated Land%基于耕地综合价值的土地征收补偿标准研究

    Institute of Scientific and Technical Information of China (English)

    诸培新; 卜婷婷; 吴正廷

    2011-01-01

    The existing compensation of cultivated land expropriation only covers cultivated land value of agricultural use and employment, and neglects other values such as ecological value, which leads to a series of social and economic problems related to cultivated land expropriation. Therefore, it is necessary to protect farmers' land rights and cultivated land resources to establish a reasonable standard of cultivated land expropriation. Based on the theory of comprehensive value of cultivated land resources, land expropriation compensation needs to cover three kinds of value; agricultural economic value, social security value and ecological value. Then, different methods are employed to calculate various values of land. The return flow discount method is used for agricultural economic value measurement, and cost substitution method for social value calculation, and of contingent value method for ecological value. Last, Nanjing City of Jiangsu Province is taken as an example to establish expropriation compensation standard adapted to comprehensive land value in 2007. The result of the case study shows that compensation received by farmers according to comprehensive compensation standard shall be 6.6 times of the existing compensation standard for land expropriation. Because the new land compensation can internalize external value of cultivated land such as social stability value and ecological values, and effectively eliminate the market failure during the cultivated land expropriation, it will improve the efficiency of cultivated land conversion to non-agricultural use. In addition, new standard of land compensation is much lower than the current benchmark price of construction land; therefore, it is feasible in economy and acceptable in society.%由于现行耕地征收补偿仅对耕地的农业经济价值以及部分就业保障价值进行补偿,忽视了耕地的其他价值,导致了耕地征收中的一系列社会经济问题产生.因此,合理制定

  17. Tree-based ranking methods

    OpenAIRE

    Clémençon, Stéphan; Vayatis, Nicolas

    2008-01-01

    The paper investigates how recursive partitioning methods can be adapted to the bipartite ranking problem. In ranking, the pursued goal is global: based on past data, define an order on the whole input space X, so that positive instances take up the top ranks with maximum probability. The most natural way to order all instances consists of projecting the input data onto the real line through a real-valued scoring function s and use the natural order on R. The accuracy of the ordering induced ...

  18. Regional Cluster Development Measures and New Technology Based Firms Cultivation in Japan

    OpenAIRE

    Kirihata, Tetsuya

    2008-01-01

    The aim of this paper is to reveal significant factors within a cluster that influence the fostering of new technology based firms (hereafter abbreviated NTBFs) and determine which measures should be given priority in aiding regional improvement. In this paper, a questionnaire survey was conducted with project directors of regional knowledge cluster headquarters promoted by the Ministry of Education, Culture, Sports, Science and Technology, NTBFs, as well as, external specialists such as atto...

  19. Quantification of polyphenols and pharmacological analysis of water and ethanol-based extracts of cultivated agarwood leaves.

    Science.gov (United States)

    Ito, Tetsuro; Kakino, Mamoru; Tazawa, Shigemi; Watarai, Tatsuya; Oyama, Masayoshi; Maruyama, Hiroe; Araki, Yoko; Hara, Hideaki; Iinuma, Munekazu

    2012-01-01

    Mangiferin (3) and genkwanin 5-O-β-primeveroside (5) are the two major bioactive polyphenols with laxative property present in the extracts of agarwood (Aquilaria sinensis) leaves (AL). Here we developed an HPLC method to determine these bioactive components and four other major polyphenols in AL extracts and evaluated the pharmacological equivalence of organic and water extracts. Using mobile phase gradient conditions combined with UV detection at 330 nm, all six compounds were separated and we determined the relative extraction ratios of the six compounds present in A. sinensis extracts that were prepared under different conditions and compared the contents of the two laxative polyphenols present in the 60% ethanol extracts of A. sinensis and A. crassna. The polyphenols present in water extracts of 13 commercially cultivated A. crassna plants have also been analyzed. The laxative properties of 60% ethanol and four water extracts of A. crassna were evaluated by the frequency and weight of stools in loperamide-induced constipation model mice. The pharmacological equivalence of 60% ethanol extract and hot water (95°C) extract was identified in mice.

  20. Recommendations for the regionalizing of coffee cultivation in Colombia: a methodological proposal based on agro-climatic indices.

    Science.gov (United States)

    García L, Juan Carlos; Posada-Suárez, Húver; Läderach, Peter

    2014-01-01

    The Colombian National Federation of Coffee Growers (FNC) conducted an agro-ecological zoning study based on climate, soil, and terrain of the Colombian coffee-growing regions (CCGR) located in the tropics, between 1° and 11.5° N, in areas of complex topography. To support this study, a climate baseline was constructed at a spatial resolution of 5 km. Twenty-one bioclimatic indicators were drawn from this baseline data and from yield data for different coffee genotypes evaluated under conditions at eight experimental stations (ESs) belonging to the National Center for Coffee Research (CENICAFÉ). Three topographic indicators were obtained from a digital elevation model (DEM). Zoning at a national level resulted in the differentiation of 12 agro-climatic zones. Altitude notably influenced zone differentiation, however other factors such as large air currents, low-pressure atmospheric systems, valleys of the great rivers, and physiography also played an important role. The strategy of zoning according to coffee-growing conditions will enable areas with the greatest potential for the development of coffee cultivation to be identified, criteria for future research to be generated, and the level of technology implementation to be assessed.

  1. Recommendations for the regionalizing of coffee cultivation in Colombia: a methodological proposal based on agro-climatic indices.

    Science.gov (United States)

    García L, Juan Carlos; Posada-Suárez, Húver; Läderach, Peter

    2014-01-01

    The Colombian National Federation of Coffee Growers (FNC) conducted an agro-ecological zoning study based on climate, soil, and terrain of the Colombian coffee-growing regions (CCGR) located in the tropics, between 1° and 11.5° N, in areas of complex topography. To support this study, a climate baseline was constructed at a spatial resolution of 5 km. Twenty-one bioclimatic indicators were drawn from this baseline data and from yield data for different coffee genotypes evaluated under conditions at eight experimental stations (ESs) belonging to the National Center for Coffee Research (CENICAFÉ). Three topographic indicators were obtained from a digital elevation model (DEM). Zoning at a national level resulted in the differentiation of 12 agro-climatic zones. Altitude notably influenced zone differentiation, however other factors such as large air currents, low-pressure atmospheric systems, valleys of the great rivers, and physiography also played an important role. The strategy of zoning according to coffee-growing conditions will enable areas with the greatest potential for the development of coffee cultivation to be identified, criteria for future research to be generated, and the level of technology implementation to be assessed. PMID:25436456

  2. Recommendations for the regionalizing of coffee cultivation in Colombia: a methodological proposal based on agro-climatic indices.

    Directory of Open Access Journals (Sweden)

    Juan Carlos García L

    Full Text Available The Colombian National Federation of Coffee Growers (FNC conducted an agro-ecological zoning study based on climate, soil, and terrain of the Colombian coffee-growing regions (CCGR located in the tropics, between 1° and 11.5° N, in areas of complex topography. To support this study, a climate baseline was constructed at a spatial resolution of 5 km. Twenty-one bioclimatic indicators were drawn from this baseline data and from yield data for different coffee genotypes evaluated under conditions at eight experimental stations (ESs belonging to the National Center for Coffee Research (CENICAFÉ. Three topographic indicators were obtained from a digital elevation model (DEM. Zoning at a national level resulted in the differentiation of 12 agro-climatic zones. Altitude notably influenced zone differentiation, however other factors such as large air currents, low-pressure atmospheric systems, valleys of the great rivers, and physiography also played an important role. The strategy of zoning according to coffee-growing conditions will enable areas with the greatest potential for the development of coffee cultivation to be identified, criteria for future research to be generated, and the level of technology implementation to be assessed.

  3. Characterizing diversity based on nutritional and bioactive compositions of yam germplasm (Dioscorea spp. commonly cultivated in China

    Directory of Open Access Journals (Sweden)

    Zhi-Gang Wu

    2016-04-01

    Full Text Available Yams (Dioscorea spp. are widely cultivated as edible resources and medical materials in China. Characterizing chemical compositions in yam germplasm is crucial to determine their diversity and suitability for food and medicine applications. In this study, a core germplasm containing 25 yam landraces was used to create an effective classification of usage by characterizing their nutritive and medicinal compositions. All studied landraces exhibited high contents of starch from 60.7% to 80.6% dry weight (DW, protein (6.3–12.2% DW, minerals (especially Mg 326.8–544.7 mg/kg DW, and essential amino acids. Allantoin and dioscin varied considerably, with values of 0.62–1.49% DW and 0.032–0.092% DW, respectively. The quality variability of 25 yam landraces was clearly separated in light of UPGMA clustering and principal component analysis (PCA. Using an eigenvalue ≥1 as the cutoff, the first three principal components accounted for most of the total variability (62.33%. Classification was achieved based on the results of the measured parameters and principal component analysis scores. The results are of great help in determining appropriate application strategies for yam germplasm in China.

  4. Fruit protected cultivation in China

    Institute of Scientific and Technical Information of China (English)

    Gao Huajun; Wang Saoming; Wang Jiaxi

    2003-01-01

    Protected fruit cultivation in China has developed very quickly from the early 1990s, and now it is animportant branch in fruit cultivation. A brief review including fruit species, developing history, growing area, output, anddistribution in the whole country is made in the paper. Characteristics of the dominant kinds of greenhouse,environmental control methods, and standards of temperature, humidity, light and CO2 for different fruit species arepresented. Information on varieties, growing benefits, special management practices and other aspects of the main fruitspecies used for protected cultivation are also presented.

  5. Cultivating strategic thinking skills.

    Science.gov (United States)

    Shirey, Maria R

    2012-06-01

    This department highlights change management strategies that may be successful in strategically planning and executing organizational change initiatives. With the goal of presenting practical approaches helpful to nurse leaders advancing organizational change, content includes evidence-based projects, tools, and resources that mobilize and sustain organizational change initiatives. In this article, the author presents an overview of strategic leadership and offers approaches for cultivating strategic thinking skills.

  6. DISPLACEMENT BASED SEISMIC DESIGN METHODS.

    Energy Technology Data Exchange (ETDEWEB)

    HOFMAYER,C.MILLER,C.WANG,Y.COSTELLO,J.

    2003-07-15

    A research effort was undertaken to determine the need for any changes to USNRC's seismic regulatory practice to reflect the move, in the earthquake engineering community, toward using expected displacement rather than force (or stress) as the basis for assessing design adequacy. The research explored the extent to which displacement based seismic design methods, such as given in FEMA 273, could be useful for reviewing nuclear power stations. Two structures common to nuclear power plants were chosen to compare the results of the analysis models used. The first structure is a four-story frame structure with shear walls providing the primary lateral load system, referred herein as the shear wall model. The second structure is the turbine building of the Diablo Canyon nuclear power plant. The models were analyzed using both displacement based (pushover) analysis and nonlinear dynamic analysis. In addition, for the shear wall model an elastic analysis with ductility factors applied was also performed. The objectives of the work were to compare the results between the analyses, and to develop insights regarding the work that would be needed before the displacement based analysis methodology could be considered applicable to facilities licensed by the NRC. A summary of the research results, which were published in NUREGICR-6719 in July 2001, is presented in this paper.

  7. Robot-based Learning : Toward Cultivation of Information Technology Skills for Mechanical Engineering Students

    Science.gov (United States)

    Hanajima, Naohiko; Yamashita, Mitsuhisa; Kazama, Toshiharu; Yuasa, Tomonori; Niida, Yoichi; Aizu, Yoshihisa; Hikita, Hiromitsu

    Today there is increasing development of products in which embedded microprocessors are installed in a wide variety of industrial fields including mechanical industries. Mechanical engineers will be asked to develop such embedded systems in the future. To educate mechanical engineering students who possess information technology skills, for five years we have offered practical classes in which the students build a mobile robot with an embedded microprocessor and compete in a robot-triathlon race. The students have an incentive to program their robot to finish the race. We call this style of learning “robot-based learning.” In this paper, we discuss the efficiency of and problems in our practical classes as derived from information gained in surveys. In addition, we verify how the engineering design abilities of the students are improved through their participation in these classes.

  8. Potential evaluation method on plow layer soil-stripping of cultivated land occupied by non-agricultural construction%非农建设占用耕地耕层土壤剥离潜力评价方法

    Institute of Scientific and Technical Information of China (English)

    陈心佩; 钟守琴; 魏朝富

    2016-01-01

    proposed a quantitative evaluation method about the potential of the plow layer soil-stripping of the cultivated land occupied by non-agricultural construction, and Jiangjin District of Chongqing was chosen as an example. Firstly, in order to ensure that plow layer soil-stripping was fertile, the indictors including organic matter, alkali-hydrolysable nitrogen (N), available phosphorus (P), available potassium (K), total N, total P and total K were chosen to evaluate the quality of plow layer soil in Jiangjin District. The fuzzy mathematical evaluation method was used in the determination of the membership degree about evaluation indicators of plow layer soil quality. The analysis hierarchy process was used in the determination of the weight of evaluation indicators. The fuzzy set weighted synthesis method was applied to establish a comprehensive evaluation model for plow layer soil quality in Jiangjin District. Based on ArcGIS, the quality of plow layer soil in Jiangjin District was discussed by using the ordinary Kriging method, which was divided into 5 levels. Then, in order to save the financial resource and human resource, reduce the project cost and realize the scale stripping and improve the benefit of project, the indicators of slope, soil thickness, distance to lane and single plot area were chosen to evaluate the quality of cultivated land occupied by non-agricultural construction in Jiangjin District. By using the Superposition Tool and Statistical Tool in Analysis Tools of ArcGIS, the cultivated land occupied by construction land in Jiangjin District from 2013 to 2020 could be inferred. With reference to the evaluation indicators of cultivatedland quality, the cultivated land occupied by non-agricultural construction in Jiangjin District could be divided into 5 levels. Finally, according to the level of plow layer soil quality and the level of cultivated land quality, the potential of plow layer soil-stripping in Jiangjin District could be calculated and the

  9. Exploring the complex response to linuron of bacterial communities from biopurification systems by means of cultivation-independent methods.

    Science.gov (United States)

    Dealtry, Simone; Nour, Eman H; Holmsgaard, Peter N; Ding, Guo-Chun; Weichelt, Viola; Dunon, Vincent; Heuer, Holger; Hansen, Lars H; Sørensen, Søren J; Springael, Dirk; Smalla, Kornelia

    2016-02-01

    On-farm biopurification systems (BPSs) treat pesticide-contaminated wastewater at farms through biodegradation and sorption processes. However, information on the microbiota involved in pesticide removal in BPSs is scarce. Here we report on the response of BPS bacterial communities to the herbicide linuron (BPS(+)) compared with the control (BPS(-)) in a microcosm experiment. Both denaturing gradient gel electrophoresis (DGGE) and pyrosequencing of 16S rRNA gene fragments amplified from community DNA indicated shifts in the bacterial community after linuron application. Responding populations belonged to taxa that were previously reported from linuron degrading consortia cultivated from soil (Hyphomicrobiaceae, Comamonadaceae, Micrococcaceae). In addition, numerous taxa with increased relative abundance were identified that were previously not associated with linuron degradation. The relative abundance of IncP-1 korB copies increased in response to linuron application. Amplicon pyrosequencing of IncP-1 trfA genes revealed a high IncP-1 plasmid diversity and suggested that populations carrying IncP-1β plasmids increased in relative abundance. Transferable mercury resistance plasmids were exogenously captured from BPS(+)/BPS(-), and in three transconjugants from BPS(+) the gene hylA was detected. Our data suggest the existence of a multispecies linuron degrading bacterial food web and an involvement of IncP-1 plasmids in the adaptation of bacterial communities to pesticide pollution in BPSs. PMID:26705572

  10. CULTIVATION OF CLADOCERAN (CLADOCERA FOR INCREASING PROVISION OF YOUNG-OF-THE-YEAR CARP (CYPRINUS CARPIO WITH NATURAL FEEDS (REVIEW

    Directory of Open Access Journals (Sweden)

    A. Tuchapska

    2014-06-01

    Full Text Available Purpose. Natural feeds are important in pond fish diet because they contain all nutrients necessary for fish growth and development. The share of natural feeds in fish diet has great effect on fish growth and immunity, assimilation of artificial feeds. The main way of assured procurement of natural feeds for fish feeding at different stages of their development is artificial cultivation of aquatic organisms. However, cultivation of natural feeds is not virtually performed in aquaculture enterprises of Ukraine, therefore an analysis of available data on zooplankton cultivation is important for looking for optimal and economically profitable methods of enrichment of pond fish diet with natural feeds. Methodology. Methods of cladoceran cultivation were the object of the study, material for the study – literature data on ways and methods of zooplankton cultivation. Findings. Cultivation of various species of zooplankton is performed for feeding of pond fish on different life stages. Main object of cultivation in aquaculture is Daphnia magna Straus, juvenile forms of which are consumed by fish larvae, while adult organisms are the most valuable for yearlings and older fish. The efficiency of hydrobiont cultivation highly depends on the selected object, containers, where cladocerans are cultivated, optimum conditions, peculiarities of water supply, species, and application of fertilizers and feeds. Originality. The highest production of zooplankton can be obtained when cultivating D. magna in tanks with continuous flow and in net cages installed in ponds under condition of ensuring requirements of the culture in bacterial and algae feeds (due to application of fertilizers and feeding with feeds and microalgae taking into account their presence in water, which is in the tank-cultivator. Practical value. Simplicity of the methods and high efficiency of zooplankton cultivation for preparation of pond fish juveniles is the basis of its wide use in

  11. Cultivating Engineering Ethics and Critical Thinking: A Systematic and Cross-Cultural Education Approach Using Problem-Based Learning

    Science.gov (United States)

    Chang, Pei-Fen; Wang, Dau-Chung

    2011-01-01

    In May 2008, the worst earthquake in more than three decades struck southwest China, killing more than 80,000 people. The complexity of this earthquake makes it an ideal case study to clarify the intertwined issues of ethics in engineering and to help cultivate critical thinking skills. This paper first explores the need to encourage engineering…

  12. Dynamic of China's cultivated land and landcover changes of its typical regions based on remote sensing data

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Using the multi-temporal Landsat data and survey data of national resources, the authors studied the dynamics of cultivated land and landcover changes of typical ecological regions in China. The results of investigation showed that the whole distribution of the cultivated land shifted to Northeast and Northwest China, and as a result, the ecological quality of cultivated land dropped down. The seacoast and cultivated land in the area of Yellow River Mouth expanded by an increasing rate of 0.73 km?a-1, with a depositing rate of 2.1 km?a-1. The desertification area of the dynamic of Horqin Sandy Land increased from 60.02% of the total land area in1970s to 64.82% in1980s but decreased to 54.90% in early 1990s. As to the change of North Tibet lakes, the water area of the Namu Lake decreased by 38.58 km2 from year 1970 to 1988, with a decreasing rate of 2.14 km2?a-1.

  13. Cellulase and xylanase productions by isolated Amazon Bacillus strains using soybean industrial residue based solid-state cultivation

    Directory of Open Access Journals (Sweden)

    Heck Júlio X.

    2002-01-01

    Full Text Available In Brazil, a large amount of a fibrous residue is generated as result of soybean (Glycine max protein production. This material, which is rich in hemicellulose and cellulose, can be used in solid state cultivations for the production of valuable metabolites and enzymes. In this work, we studied the bioconversion of this residue by bacteria strains isolated from water and soil collected in the Amazon region. Five strains among 87 isolated bacteria selected for their ability to produce either celullases or xylanases were cultivated on the aforementioned residue. From strain BL62, identified as Bacillus subtilis, it was obtained a preparation showing the highest specific cellulase activity, 1.08 UI/mg protein within 24 hours of growth. Concerning xylanase, the isolate BL53, also identified as Bacillus subtilis, showed the highest specific activity for this enzyme, 5.19 UI/mg protein within 72 hours of cultivation. It has also been observed the production of proteases that were associated with the loss of cellulase and xylanase activities. These results indicated that the selected microorganisms, and the cultivation process, have great biotechnological potential.

  14. Research on the Cultivation Mechanism of Innovative Talents Based on TPB%基于 TPB的创新型人才培养机制研究

    Institute of Scientific and Technical Information of China (English)

    孔德议

    2015-01-01

    The cultivation of innovative talents has a profound significance for China in transition. The paper analyzes innovative talent training mechanism and universities based on the theory of planned behavior (TPB) to explore how to cultivate talents to satisfy the needs of the society un‐der the existing circumstances in China. The paper analyzes the influence factors of creative talents training from the subjective norm ,behavioral attitude and perceived behavior control three aspects respectively. And then it constructs the innovative talent cultivation mechanism based on TPB model and puts forward the improvement suggestions and countermeasures of cultivating innovative talents in colleges and universities .%创新型人才的培养对处于转型中的中国有着深远的意义。基于计划行为理论(简称T PB )对创新型人才培养机制进行分析研究,旨在探索在中国现有的大环境下如何培养出适应社会需要的人才。从主观规范,行为态度和感知行为控制三方面分别对应分析创新型人才培养的影响因素,在此基础上构建了基于 T PB的创新型人才培养机制模型并提出改进培养创新型人才的相关建议和对策。

  15. Cultivating nutrition

    OpenAIRE

    Levin, Carol E.; Long, Jennifer; Simler, Kenneth R.; Johnson-Welch, Charlotte

    2003-01-01

    "Over the past decade, donor-funded policies and programs designed to address undernutrition in the Global South have shifted away from agriculture-based strategies toward nutrient supplementation and food fortification programs. Given the potential benefits resulting from agriculture-based nutrition interventions, this study uses Q methodology to explore the views of a range of stakeholders from both developed and developing countries on the value of—and constraints related to—gender-sensiti...

  16. Bioreactors for high cell density and continuous multi-stage cultivations: options for process intensification in cell culture-based viral vaccine production.

    Science.gov (United States)

    Tapia, Felipe; Vázquez-Ramírez, Daniel; Genzel, Yvonne; Reichl, Udo

    2016-03-01

    With an increasing demand for efficacious, safe, and affordable vaccines for human and animal use, process intensification in cell culture-based viral vaccine production demands advanced process strategies to overcome the limitations of conventional batch cultivations. However, the use of fed-batch, perfusion, or continuous modes to drive processes at high cell density (HCD) and overextended operating times has so far been little explored in large-scale viral vaccine manufacturing. Also, possible reductions in cell-specific virus yields for HCD cultivations have been reported frequently. Taking into account that vaccine production is one of the most heavily regulated industries in the pharmaceutical sector with tough margins to meet, it is understandable that process intensification is being considered by both academia and industry as a next step toward more efficient viral vaccine production processes only recently. Compared to conventional batch processes, fed-batch and perfusion strategies could result in ten to a hundred times higher product yields. Both cultivation strategies can be implemented to achieve cell concentrations exceeding 10(7) cells/mL or even 10(8) cells/mL, while keeping low levels of metabolites that potentially inhibit cell growth and virus replication. The trend towards HCD processes is supported by development of GMP-compliant cultivation platforms, i.e., acoustic settlers, hollow fiber bioreactors, and hollow fiber-based perfusion systems including tangential flow filtration (TFF) or alternating tangential flow (ATF) technologies. In this review, these process modes are discussed in detail and compared with conventional batch processes based on productivity indicators such as space-time yield, cell concentration, and product titers. In addition, options for the production of viral vaccines in continuous multi-stage bioreactors such as two- and three-stage systems are addressed. While such systems have shown similar virus titers compared to

  17. Bioreactors for high cell density and continuous multi-stage cultivations: options for process intensification in cell culture-based viral vaccine production.

    Science.gov (United States)

    Tapia, Felipe; Vázquez-Ramírez, Daniel; Genzel, Yvonne; Reichl, Udo

    2016-03-01

    With an increasing demand for efficacious, safe, and affordable vaccines for human and animal use, process intensification in cell culture-based viral vaccine production demands advanced process strategies to overcome the limitations of conventional batch cultivations. However, the use of fed-batch, perfusion, or continuous modes to drive processes at high cell density (HCD) and overextended operating times has so far been little explored in large-scale viral vaccine manufacturing. Also, possible reductions in cell-specific virus yields for HCD cultivations have been reported frequently. Taking into account that vaccine production is one of the most heavily regulated industries in the pharmaceutical sector with tough margins to meet, it is understandable that process intensification is being considered by both academia and industry as a next step toward more efficient viral vaccine production processes only recently. Compared to conventional batch processes, fed-batch and perfusion strategies could result in ten to a hundred times higher product yields. Both cultivation strategies can be implemented to achieve cell concentrations exceeding 10(7) cells/mL or even 10(8) cells/mL, while keeping low levels of metabolites that potentially inhibit cell growth and virus replication. The trend towards HCD processes is supported by development of GMP-compliant cultivation platforms, i.e., acoustic settlers, hollow fiber bioreactors, and hollow fiber-based perfusion systems including tangential flow filtration (TFF) or alternating tangential flow (ATF) technologies. In this review, these process modes are discussed in detail and compared with conventional batch processes based on productivity indicators such as space-time yield, cell concentration, and product titers. In addition, options for the production of viral vaccines in continuous multi-stage bioreactors such as two- and three-stage systems are addressed. While such systems have shown similar virus titers compared to

  18. 非培养法检测阴道微生态的进展%Research Progress of Detecting Vagina Microecology by Cultivation-Independent Methods

    Institute of Scientific and Technical Information of China (English)

    陈晴

    2012-01-01

    女性阴道内寄生着多种微生物,鉴定阴道中存在的微生物有助于了解其在健康和疾病时的作用,但是许多微生物难以通过培养将其分离鉴定.近年来,一系列分子生物学的方法,如基于16S rRNA基因聚合酶链反应扩增法等用于研究阴道微生态并取得了较大的进展.利用分子生物学的非培养法检测女性阴道微生物发现,不论在健康女性还是细菌性阴道病(BV)患者中惰性乳杆菌是普遍存在的乳杆菌;还发现许多新的厌氧菌与BV高度相关,如阴道阿托波菌、阴道加德纳菌、BVAB1等.%The human vagina hosts many microbes. Identifying all kinds of microbes in vagina will help to better understand their roles in heath and disease. However,many microbes are difficult to be identified by cultivation-dependent methods. In recent years, a series of molecular approaches, such as PCR amplification of 16S rRNA gene,are used to study the vagina microecology and have made considerable progress. The use of molecular, cultivation-independent methods have revealed :lactobacillus inert is the ubiquitous nature in women with or without bacterial vaginosis( BV ); some novel anaerobes are strongly associated with BV, such as Atopobium vaginae, Gardnerella vaginalis, BVAB1 and so on.

  19. Multi-Suitability Evaluation of Cultivated Land in Houhu Farm Area Based on GIS and Niche-Fitness

    Institute of Scientific and Technical Information of China (English)

    LIU Li-wei; NIE Yan; ZHOU Yong

    2005-01-01

    The niche-fitness and limited factors of rice, wheat, cotton and rape in Houhu Farm area of Jianghan Plain are analyzed by weighted average model and limited factor model. The niche-fitness theory and GIS are applied to the research on multi-suitability evaluation. The high values of the niche-fitness (the maximum is 0.958) indicate that the environmental conditions basically meet the requirements of crops in the area. Suitability evaluation of single crop shows that the degrees of suitability are satisfactory. Comprehensive suitability evaluation shows that the acreage for grade 1 (highly suitable for cultivation) accounts for 18.16% of the total cultivated land area, whereas the acreage for grade 3 (critically suitable) and grade 4 (unsuitable) only for 8.9%. The main factors limiting the potential land productivity are cation exchange capacity (CEC), available phosphorus content and accessibility level of road.

  20. Plant growth and cultivation.

    Science.gov (United States)

    Podar, Dorina

    2013-01-01

    There is a variety of methods used for growing plants indoor for laboratory research. In most cases plant research requires germination and growth of plants. Often, people have adapted plant cultivation protocols to the conditions and materials at hand in their own laboratory and growth facilities. Here I will provide a guide for growing some of the most frequently used plant species for research, i.e., Arabidopsis thaliana, barley (Hordeum vulgare) and rice (Oryza sativa). However, the methods presented can be used for other plant species as well, especially if they are related to the above-mentioned species. The presented methods include growing plants in soil, hydroponics, and in vitro on plates. This guide is intended as a starting point for those who are just beginning to work on any of the above-mentioned plant species. Methods presented are to be taken as suggestive and modification can be made according to the conditions existing in the host laboratory. PMID:23073874

  1. Impacts of energy crop cultivation on nature and landscape. Development and application of an evaluation method; Auswirkungen des Energiepflanzenanbaus auf Natur und Landschaft. Entwicklung und Anwendung einer Bewertungsmethode

    Energy Technology Data Exchange (ETDEWEB)

    Wiehe, Julia

    2011-08-15

    For long-term planning, knowledge about the interrelationship of effects of the cultivation method and the sensitivity of ecological balance is essential. Hence, the objective of this thesis is the development of a method for the evaluation of the impacts of bioenergy crop production for biogas use on the natural environment. The developed method is in alignment with existing methods. It is also in alignment with those methods used within the practice of landscape planning, so that the method as well as the derived conclusions can be implemented into landscape planning practice in the future. The evaluation method has been applied in the three model regions Hildesheim, Soltau-Fallingbostel and Emsland. These test areas represent different physical regions in Lower Saxony and typical agricultural production conditions. On the basis of these results, general statements on the impact of bioenergy crop production on the ecological balance of the area can be made. [German] Grundlage fuer eine vorausschauende Steuerung des Ausbaus erneuerbarer Energien ist die Kenntnis der Zusammenhaenge der Wirkung des Energiepflanzenanbaus und der Empfindlichkeit des Naturhaushaltes. Ziel der Arbeit ist daher die Erarbeitung einer Methode zur umfassenden Bewertung dieser Auswirkungen auf den Naturhaushalt. Die Methode orientiert sich an bereits bestehenden und in der Praxis der Landschaftsplanung angewendeten Bewertungsmethoden, so dass sie ebenso wie die daraus abgeleiteten Schlussfolgerungen zukuenftig Eingang in die Planungspraxis finden kann. Die Bewertungsmethode wird in den drei Modellregionen Hildesheim, Soltau-Fallingbostel und Emsland angewendet, mit denen die verschiedenen Naturraeume und fuer Niedersachsen typischen landwirtschaftlichen Produktionsbedingungen abgebildet werden. Auf Grundlage dieser Ergebnisse koennen dann allgemeine Aussagen zu den Auswirkungen des Energiepflanzenanbaus auf den Naturhaushalt gemacht werden.

  2. Research on Monitoring Area Division of Quality Grade Changes in County Cultivated Land and Technology of Deploying Monitoring Point

    Institute of Scientific and Technical Information of China (English)

    Wei; WEI; Lijun; LIAO; Jianxin; YU

    2013-01-01

    It is an important means in management of improving both the quality and quantity of cultivated land to monitor grade changes in cultivated land quality. How to deploy monitoring network system and its point reasonably and roundly are the key to the technology of monitoring grade changes in cultivated land quality by monitoring grade changes in cultivated land quality dynamically in order to obtain the information to the index of cultivated land quality and its changes based on the existing achievements of farmland classification and grading. Spatial analysis method is used to demarcate monitoring area and deploy monitoring point according to ARCGIS,of which the result can meet the demand for monitoring grade changes in cultivated land.

  3. COMPANY VALUATION METHODS BASED ON PATRIMONY

    Directory of Open Access Journals (Sweden)

    SUCIU GHEORGHE

    2013-02-01

    Full Text Available The methods used for the company valuation can be divided into 3 main groups: methods based on patrimony,methods based on financial performance, methods based both on patrimony and on performance. The companyvaluation methods based on patrimony are implemented taking into account the balance sheet or the financialstatement. The financial statement refers to that type of balance in which the assets are arranged according to liquidity,and the liabilities according to their financial maturity date. The patrimonial methods are based on the principle thatthe value of the company equals that of the patrimony it owns. From a legal point of view, the patrimony refers to allthe rights and obligations of a company. The valuation of companies based on their financial performance can be donein 3 ways: the return value, the yield value, the present value of the cash flows. The mixed methods depend both onpatrimony and on financial performance or can make use of other methods.

  4. Comparison of soil thermal properties in cultivated fields determined using soil water content measured by two methods

    OpenAIRE

    Kossowski J.; Usowicz B.

    1999-01-01

    Results of the measurements of water content in the topsoil layer (1-6 cm) in fields with various crops obtained by gravimetric and reflectometric (TDR) methods have been used for the calculations of soil volumetric heat capacity, thermal conductivity, and diffusivity. Calculation values of individual soil thermal properties obtained in the two ways were then analysed by means of statistical and geostatistical methods and compared (correlation coefficients, regression equations, difference di...

  5. In vitro cultivation of rat bone marrow mesenchymal stem cells and establishment of pEGFP/Ang-1 transfection method

    Institute of Scientific and Technical Information of China (English)

    Xiu-Qun; Zhang; Long; Wang; Shu-Li; Zhao; Wei; Xu

    2014-01-01

    Objective:To obtain the bone marrow mesenchymal stem cells(BMSCs).complete phenotypic identification and successfully transfecl rat BMSCs by recombinant plasmid pF.GFP/Ang-1.Methods:BMSCs were isolated from bone marrow using density gradient centrifugation method and adherence screening method,and purified.Then the recombinant plasmid pEGFP/Ang-1was used to transfect BMSCs and the positive clones were obtained by the screen of C418 and observed under light microscopy inversely.Green fluorescent exhibited by protein was enhanced to measure the change time of the expression amount of Ang-1.Results:BMSCs cell lines were obtained successfully by adherence screening method and density gradient ccntrifugation.Ang-1 recombinant plasmid was transfected smoothly into rat BMSCs,which can express Ang-1 for 3 d and decreased after 7 d.Conclusions:Adherence screening method und density gradient ceiilrifugation can be effective methods lo obtain BMSCs with high purity and rapid proliferation.Besides,the expression of transfected recombinant plasmid pEGFP/Ang-1 in rat BMSCs is satisfactory.

  6. Based on Penalty Function Method

    Directory of Open Access Journals (Sweden)

    Ishaq Baba

    2015-01-01

    Full Text Available The dual response surface for simultaneously optimizing the mean and variance models as separate functions suffers some deficiencies in handling the tradeoffs between bias and variance components of mean squared error (MSE. In this paper, the accuracy of the predicted response is given a serious attention in the determination of the optimum setting conditions. We consider four different objective functions for the dual response surface optimization approach. The essence of the proposed method is to reduce the influence of variance of the predicted response by minimizing the variability relative to the quality characteristics of interest and at the same time achieving the specific target output. The basic idea is to convert the constraint optimization function into an unconstraint problem by adding the constraint to the original objective function. Numerical examples and simulations study are carried out to compare performance of the proposed method with some existing procedures. Numerical results show that the performance of the proposed method is encouraging and has exhibited clear improvement over the existing approaches.

  7. PBL教学法在实验教学中对学生批判性思维能力的培养%Application of PBL teaching method in cultivation of critical thinking ability of students in experimental teaching

    Institute of Scientific and Technical Information of China (English)

    秦海宏; 葛伟; 孙雪洁

    2013-01-01

    The PBL teaching method is a teaching mode based on problems.It has significant effect on the formation of crilical thinking ability of the nursery students in experimental teaching.In our study,the PBL was adopted in the experimental group,while traditional teaching method was used in the control group.CCTDI-CV was applied to assess the critical thinking ability of the students in these above two groups.It was found that the critical thinking ability of the students in the experimental group is significantly higher than that in the control group.This suggested that the PBL is beneficial to cultivate the critical thinking ability of the students.This paper provides an evidence for improving the teaching mode and way of the cultivation of critical thinking ability in nursery practice teaching.%PBL教学是以问题为基础的模式,在实验教学中对培养学生批判性思维能力的效果显著.在对实验组采用PBL教学法教学、对照组采用传统的实验教学方法教学的基础上,应用CCTDI-CV评价2种教学方法对学生批判性思维能力的影响.经分析发现,PBL教学组学生的批判性思维能力高于对照组,两组间差异显著.实验教学中的PBL教学模式有助于学生批判性思维能力的培养.

  8. Venturini Method Based Matrix Converter

    Directory of Open Access Journals (Sweden)

    Derick Mathew

    2015-03-01

    Full Text Available Recently, matrix converter has received considerable interest as a viable alternative to the conventional ac-dc-ac converter. This direct ac-ac converter provides some attractive characteristics such as: four quadrant operation, absence of bulky dc-link electrolyte capacitors, clean input power characteristics. Due to the absence of dc link energy storage elements any disturbance in the input voltage will be immediately reflected to the output voltages. In this paper venturini method for matrix converter has been presented. Three phase sinusoidal symmetrical voltage or current can obtained .

  9. Evaluation on Cultivated Land Ecological Security of Leshan City Based on Improved Set Pair Analysis%基于改进 SPA 的乐山市耕地生态安全评价

    Institute of Scientific and Technical Information of China (English)

    刘小波; 秦天彬; 周宝同; 涂建军

    2016-01-01

    Evaluation of cultivated land ecological security is comprehensive diagnosis for complex system of farmland ecosystem ,and it is an important content to measure regional sustainable development .T his pa‐per takes Leshan City of Sichuan province for example ,on the basis of defining the connotation of cultivat‐ed land ecological security ,constructing an evaluation index system base on the Pressure‐State‐Response (PSR)model .With the improved SPA the cultivated land ecological security in Leshan City and its districts in 2007 ,2009 ,and 2012 has been evaluated . T hen the change factors are discussed and conclusions are summarized as follow s :1) T he level of cultivated land ecological security in Leshan City is increasing on the w hole ,safe level is gradually improved from “critically safe”to“safe” ,the level of cultivated land eco‐logical security in each district is improved in varying degree .2) The quality and quantity of cultivated land resources ,the good points and bad points of ecological environment ,and the level of economy are the main factors which lead to the differences of cultivated land ecological security between the south and the north . 3) The improved SPA method can reveal the degree of differentiation of evaluation index system for culti‐vated land ecological security ,and extract the intermediate information of comprehensive evaluation re‐sults ,w hich has some practical value in the evaluation of cultivated land ecological security .%耕地生态安全评价是对农田生态复合系统的全面诊断,也是测度区域可持续发展的重要内容。本文以四川省乐山市为例,在界定耕地生态安全内涵的基础上,构建基于PSR模型的评价指标体系,并采用改进的SPA法分别对乐山市及各区县2007,2009,2012年耕地生态安全进行评价,并对其变化因素进行了分析。结果表明:1)乐山市耕地生态安全水平整体上呈上升趋势,

  10. Objective, Way and Method of Faculty Management Based on Ergonomics

    Science.gov (United States)

    WANG, Hong-bin; Liu, Yu-hua

    2008-01-01

    The core problem that influences educational quality of talents in colleges and universities is the faculty management. Without advanced faculty, it is difficult to cultivate excellent talents. With regard to some problems in present faculty construction of colleges and universities, this paper puts forward the new objectives, ways and methods of…

  11. An interactive segmentation method based on superpixel

    DEFF Research Database (Denmark)

    Yang, Shu; Zhu, Yaping; Wu, Xiaoyu

    2015-01-01

    This paper proposes an interactive image-segmentation method which is based on superpixel. To achieve fast segmentation, the method is used to establish a Graphcut model using superpixels as nodes, and a new energy function is proposed. Experimental results demonstrate that the authors' method has...... excellent performance in terms of segmentation accuracy and computation efficiency compared with other segmentation algorithm based on pixels....

  12. Digital Autofocusing Method Based on Contourlet Transform

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The autofocusing technique based on contourlet transform is discussed in this paper and an autofocusing method is proposed for images with much information in certain directions. The experimental results show that theproposed method can focus accurately and the sensitivity ratio is higher than that of the other autofocusing methods based on conventional image processing

  13. 高职人才培养机理研究:产业集群创新系统的视角%Research on Talent Cultivation Mechanism of the Higher Vocational Education Based on Industry Cluster Groups

    Institute of Scientific and Technical Information of China (English)

    毛才盛; 吕慈仙

    2012-01-01

    本文通过梳理产业集群创新系统相关理论,结合高职人才培养模式特征,基于产业集群与人才培养的耦合机理结构,提出了产业集群与人才培养的耦合度模型,分析产业集群下高职人才培养模式存在着专业设置与市场需求脱节、人才培养资源共享机制不健全、产业与人才培养互动机制缺失等问题,并提出实现产业集群与人才培养的良性耦合机制的对策。%With analysing industry cluster groups theory and talent cultivation features of high vocational education,coupling mechanism between industry cluster groups and talent cultivation,this paper proposes the coupling model of industry cluster groups and talent cultivation,analyses the problems existing in higher vocational talent cultivation based on the industry cluster groups such as no tight coupling between professional setting and market demand,no strong talent cultivation resource sharing mechanism,no information sharing mechanism between industry and talent cultivation,and puts forward the suggestions on how to realize the coupling mechanism between industry cluster and talent cultivation.

  14. Effect of plant cultivation methods on content of major and trace elements in foodstuffs and retention in rats

    DEFF Research Database (Denmark)

    Kristensen, Mette; Østergaard, Lars F.; Halekoh, Ulrich;

    2008-01-01

    major and trace element contents of dried foodstuffs (carrots, kale, peas, potatoes and apples) grown in two consecutive years, as well as mineral retention determined in 36 rats (second generation in a multi-generation study) fed diets based on these foodstuffs from one year. RESULTS: Overall...

  15. Exploratory research on course teaching of architectural design based on ability cultivation of practice and innovation - Take course teaching of high-rise building design as an example

    Directory of Open Access Journals (Sweden)

    Gao Ming

    2016-01-01

    Full Text Available Under current disciplinary development reform and the objective of strengthening ability cultivation of practice innovation, this thesis makes comprehensive analysis of the problems existing in “high-rise building design” teaching for undergraduates majoring in architecture; and proposes new philosophies of focusing on teaching process, opening up joint school management and research-oriented teaching in combination of construction transformation demands. Based on the above background, this thesis also proposes the teaching conception of establishing multi-platform frame. Moreover, it discusses corresponding platform establishment and teaching design in combination of educational reform practice.

  16. 县域耕地后备资源与规划期内数量质量并重的占补平衡分析%Analysis on county based reserved resource for cultivated land and quality-quantity requisition-compensation balance in planning period

    Institute of Scientific and Technical Information of China (English)

    高星; 吴克宁; 郧文聚; 唐程杰; 许实; 麻战洪

    2015-01-01

    Reserved resource for cultivated land is an important supplement to cultivated land, and it plays an important role in requisition-compensation balance, however, it has disadvantages of subjectiveness and emphasizing on quantity in cultivated land supplement. It is necessary to play the role of farmland reserve resources to replenish cultivated land for the realization of the goal of farmland protection in the overall planning period of county land use. Data used for study are from the results of the new round of ‘survey and evaluation of reserved resource for cultivated land’. A balancing method, which focused on both quantity and quality and was based on the ratio of added cultivated land and grade conversion coefficient, were proposed to analyze the capability of requisition-compensation balance in the planning period. And we analyzed the capacity to achieve the balance of arable land in the county planning period. The coefficient of newly increased cultivated land was developed by the weighted average of the newly cultivated land rate. The conversion coefficient of requisition and compensation of arable land is based on the national unified research results of arable land’s requisition compensation balance according to grade conversion coefficient from Land Resources and Land Rehabilitation Center. Taking Yuanjiang City, Hunan Province as an example, we calculated the area of reserved resource for cultivated land needed for realizing both quantitative and qualitative balance. According to the "Yuanjiang City land use overall planning (during 2006-2020)", the new construction area of farmland was expected to not more than 899.2 hm2 until 2020, the quality of new construction occupation of cultivated land focused on the level of 1-5, whose composition ratios were 40.61%, 16.15%, 22.73%, 18.8% and 1.72%, respectively. The reserved resource area of cultivated land in Yuanjiang City was 1 744.14 hm2, of which 1 447.64 hm2 cultivated land can be added after the

  17. GIS-based assessment of land suitability for alfalfa cultivation: a case study in the dry continental steppes of northern China

    Directory of Open Access Journals (Sweden)

    Fei Deng

    2014-03-01

    Full Text Available Alfalfa (Medicago sativa L. is the most valuable perennial forage grass in northern China. We selected 12 ecological criteria and 4 socioeconomic criteria to calculate the suitability of land for alfalfa cultivation in the Xilingol League of the Inner Mongolia Autonomous Region. We combined ecological suitability assessment with fuzzy analysis to standardize the criteria. We used the analytical hierarchy process to determine the weight of these criteria, and used multi-criterion decision analysis (MCDA to aggregate the criteria. We then calculated the suitability score for each evaluation unit. The suitability was divided into highly, moderately, and marginally suitable, or unsuitable, using the geoprocessing module of ArcGIS 9.3 according to the FAO land suitability classification. We found that highly suitable areas covered 10,799.84 km2, accounting for 5.3% of the total area, and unsuitable areas covered 99,235.87 km2, accounting for 48.9% of the total area. Combining the fuzzy analysis method with the theory of ecological suitability and the MCDA method to evaluate the suitability of land for alfalfa cultivation provided insights that can guide decisionmakers and farmers to make more practical and scientific decisions.

  18. High-density Linkage Map of Cultivated Allotetraploid Cotton Based on SSR, TRAP, SRAP and AFLP Markers

    Institute of Scientific and Technical Information of China (English)

    Jiwen Yu; Shuxun Yu; Cairui Lu; Wu Wang; Shuli Fan; Meizhen Song; Zhongxu Lin; Xianlong Zhang; Jinfa Zhang

    2007-01-01

    A high-density linkage map was constructed for an F2 population derived from an interspecific cross of cultivated allotetraploid species between Gossyplum hirsutum L. and G. barbadense L. A total of 186 F2 individuals from the interspecific cross of "CRI 36 × Hai 7124" were genotyped at 1 252 polymorphic loci including a novel marker system,target region amplification polymorphism (TRAP). The map consists of 1 097 markers, including 697 simple sequence repeats (SSRs), 171 TRAPs, 129 sequence-related amplified polymorphisms, 98 amplified fragment length polymorphisms, and two morphological markers, and spanned 4 536.7 cM with an average genetic distance of 4.1 cM per marker. Using 45 duplicated SSR loci among chromosomes, 11 of the 13 pairs of homologous chromosomes were identified in tetraplold cotton. This map will provide an essential resource for high resolution mapping of quantitative trait loci and molecular breeding in cotton.

  19. Predictive analysis on the amount of cultivated land transfer based on different targets%基于不同目标下的农户耕地流转量预测分析

    Institute of Scientific and Technical Information of China (English)

    丁敬磊; 刘光远

    2014-01-01

    基于不同假设条件和目标定位,运用实证研究和计量预测分析的方法,构建研究区农地适度经营规模测度数学模型,分阶段对研究区农户耕地流转趋势、流转量、流转率和实现预测流转率需转移的农村隐性剩余劳动力数量进行计量预测,通过预测数据与实际数据的比较,找出假设条件对本地耕地流转产生的影响程度,揭示出研究区耕地流转缓慢的根本原因,以便有针对性地制定应对之策,最大限度提高耕地的生产潜力,促进农业现代化进程。研究结果表明,在阶段2和阶段3,农户耕地可实现较为稳定而高比率的流转;而在阶段1,农户耕地只能在低比率的情况下缓慢流转,耕地流转无论从规模还是利用效率上,都存在着巨大的可流转潜力。结果表明研究区实现农户耕地适度规模经营,需要转移就业的农业劳动力数量巨大、形势紧迫、任务艰巨。%Using the method of empirical research and quantitative analysis prediction based on different assumptions and targets , the mathematical model of agricultural land proper scale management was constructed .The cultivated land transfer , circulation amount , turnover rate and the number of rural surplus labor transfer prediction of hidden rate to be transferred in Yuncheng County were forecasted by stages .Then by comparing the predicted data and the actual data , the influence of local assumptions on the cultivated land transfer was found out , the root causes of slow flow of cultivated land in Yuncheng County was revealed , and then the coping strategies were developed in order to maximize the arable land production potential and to promote the process of agricultural modernization .The results showed that, in phase 2 and phase 3, it was possible to realize the stable and high turnover rate of the cultivated land;however, the cultivated land transferred slowly at low ratios in stage 1

  20. 以能力培养为主线建设实验教学示范中心%Constructing Experimental Teaching Demonstration Center Based on Ability Cultivation

    Institute of Scientific and Technical Information of China (English)

    温武; 黄立胜; 郭旭鸣; 丘凯伦

    2011-01-01

    介绍计算机科学与工程实验教学示范中心以培养满足社会需求的复合型和创新型计算机类专业人才为目标,充分利用优越的实验条件,发挥现有师资力量,将实验教学与实践相结合,建立以能力培养为主线的"一体化、分阶段、多层次"的独立实验课程教学体系.构造"特色课程"模块,培养学生科学素养、创新意识和协作精神,开拓学生探索科学潜能的创新思路,取得了显著的成效.%To cultivate complex and innovative computer professionals, the computer science and technology experimental teaching demonstration center makes full use of favorable experimental conditions and the existing teaching staff, combines experimental teaching with practice to set up ability based experimental teaching system, which is characterized by "integration,multi-phase,multi-level". The center also constructs "special courses" module to cultivate students' scientific quality,innovation consciousness and team spirit,to develop students' exploration potential. It has achieved remarkable results.

  1. Statistical evaluation and modeling of cheap substrate-based cultivation medium of Chlorella vulgaris to enhance microalgae lipid as new potential feedstock for biolubricant.

    Science.gov (United States)

    Mohammad Mirzaie, M A; Kalbasi, M; Mousavi, S M; Ghobadian, B

    2016-05-18

    Chlorella vulgaris (C. vulgaris) microalga was investigated as a new potential feedstock for the production of biodegradable lubricant. In order to enhance microalgae lipid for biolubricant production, mixotrophic growth of C. vulgaris was optimized using statistical analysis of Plackett-Burman (P-B) and response surface methodology (RSM). A cheap substrate-based medium of molasses and corn steep liquor (CSL) was used instead of expensive mineral salts to reduce the total cost of microalgae production. The effects of molasses and CSL concentration (cheap substrates) and light intensity on the growth of microalgae and their lipid content were analyzed and modeled. Designed models by RSM showed good compatibility with a 95% confidence level when compared to the cultivation system. According to the models, optimal cultivation conditions were obtained with biomass productivity of 0.123 g L(-1) day(-1) and lipid dry weight of 0.64 g L(-1) as 35% of dry weight of C. vulgaris. The extracted microalgae lipid presented useful fatty acid for biolubricant production with viscosities of 42.00 cSt at 40°C and 8.500 cSt at 100°C, viscosity index of 185, flash point of 185°C, and pour point of -6°C. These properties showed that microalgae lipid could be used as potential feedstock for biolubricant production. PMID:25844976

  2. Reliability-based concurrent subspace optimization method

    Institute of Scientific and Technical Information of China (English)

    FAN Hui; LI Wei-ji

    2008-01-01

    To avoid the high computational cost and much modification in the process of applying traditional re-liability-based design optimization method, a new reliability-based concurrent subspace optimization approach is proposed based on the comparison and analysis of the existing muhidisciplinary optimization techniques and reli-ability assessment methods. It is shown through a canard configuration optimization for a three-surface transport that the proposed method is computationally efficient and practical with the least modification to the current de-terministic optimization process.

  3. Effects of cultivation methods on yield formation of Ningjing 3%栽培方式对宁粳3号产量形成的影响

    Institute of Scientific and Technical Information of China (English)

    王端飞; 耿春苗; 李刚华; 黎泉; 徐晶晶; 王绍华; 丁艳锋

    2011-01-01

    为探讨目前主要的栽培方式对超级稻宁粳3号产量形成的影响,于2009-2010年在江苏省丹阳市宝陵农场南京农业大学试验基地设置机插、撤播、条播、穴播、等距手栽、精量手栽和循环手栽7种栽培方式,比较各种栽培方式的产量及其构成因素、生育进程、水稻干物质积累及运转、穗型和株型特征的差异.结果表明:各处理产量表现由高到低依次为精量手栽、机插、等距手栽、撒播、循环手栽、穴播、条播.手栽高产的优势在于穗型较大,颖花数多;直播稻的劣势是每穗粒数少,颖花数较少;机插每穗粒数和颖花数介于手栽与直播之间.3种手栽方式相比较,循环手栽方式每穗粒数与颖花数明显不足,而等距手栽每穗粒数相对较少,精量手栽方式优势明显.直播方式营养生长期明显缩短,前期干物质积累过少,穗型较小,顶4叶叶片短小,产量较低.相对撒播,条播与穴播方式穗粒数较高,通过适宜的密度配置,增加穗数,有较大的增产潜力.与精量手栽相比,机插前期干物质积累较少,穗型小,提高前期干物质积累,主攻大穗,是实现高产的有效途径.%To study the effects of popular cultivation methods on yield formation in japonica rice Ningjing 3. In 2009-2010,field experiment was conducted in Baoling farm,experiment area of Nanjing Agricultural University, Danyang City,Jiangsu Province. The grain yield and its components, growth process,dry matter, panicle and plant type were analyzed in different cultivations such as machine transplanting (MT), broadcast seeding ( BS), drill seeding ( DS ) , hole-drop seeding ( HS), equidistance hand transplanting (ET) .precise hand transplanting (FT) and cycle hand transplanting(CT). The results showed that the grain yield of PT was the highest,MT was 2nd,ET was 3rd,BS was 4th,CT was 5th,HS was 6th,and DS was the lowest among these treatments. The panicle type and spikelets of hand

  4. Application of Reduced Corn Cultivation Technology in Agro-Ecosystem of Cazin Municipality

    OpenAIRE

    Mirsad Veladžić; Fatima Muhamedagić; Emdžad Galijašević

    2011-01-01

    Intensive corn cultivation is predominant in current agriculture of the Una-Sana Canton. One of the corn cultivation methods in agro-ecosystem is reduced cultivation. The paper presents the experiment of “Osmak žuti” (eight-row yellow) corn cultivation on two control sites with application of reduced and intensive cultivation in Cazin municipality. The objectives of this research were to examine the possibility of application of reduced corn cultivation; analyze statistical variation elements...

  5. 讨论式学习在麻醉住院医师培养中的应用体会%The application of discussion learning method in the cultivation of anesthetic residents

    Institute of Scientific and Technical Information of China (English)

    姚立农

    2011-01-01

    It's essential to investigate an effective learning method in order to cultivate qualified anesthetic residents Discussion learning method has the characteristics of purposiveness and systematicness, and can promote learning activeness and cultivate creativity in clinical traning.The apllication of discussion learning method depend on the topic,preparation,style of discussion.In addition,the discussion should be guided and enlightenad by experienced senior doctors and a definite conclusion must be made after discussion%讨论式学习具有很强的针对性、系统性,而且能够调动学生学习积极性、培养其创新思维.精心确定讨论主题,讨论前充分准备,采用多形式讨论方式,及时地引导启发和总结是讨论式学习顺利实施的关键.

  6. Spirulina cultivation in China

    Science.gov (United States)

    Wu, Bo-Tang; Xiang, Wen-Zhou; Zeng, Cheng-Kui

    1998-03-01

    This paper reviews and discusses the development and many problems of Spirulina cultivation in China, points out the advantages and disadvantages of open photobioreactor system, and predicts that seawater Spirulina cultivation will be a new trend to be strengthened and emphasized due to its special physiological characteristics, easier management, lower fertilizer cost, and higher resistance to contaminants and rare pollution of chemicals.

  7. 基于育人为本与能力培养为核心的教学设计与实践%Teaching design and practice based on the core of cultivation-orientation and ability training

    Institute of Scientific and Technical Information of China (English)

    张子贤

    2016-01-01

    课程建设与改革是提高教学质量的核心,基于育人为本与能力培养为核心的教学设计对提高人才培养质量具有重要的意义。阐述了基于育人为本与能力培养为核心的课程整体设计和教学设计。课程整体设计主要包括学习任务、教学模式、实现知识与能力目标的路径等方面的设计;教学设计论述了以理论支撑技术应用能力的理实一体化、思想、方法与细节的“三位一体”、教法引领学法、重视非智力因素等方面的设计。笔者将上述设计付诸于教学实践,收到了良好效果。%The course construction and reform is the core of improving teaching quality. The teaching design based on the core of cultivation-orientation and ability training has important sig-nificance to improve the quality of talents cultivation.This paper expands the overall design and teaching design based on the core of cultivation-orientation and ability training.The overall de-sign of the course indudes design of the learning task,the teaching mode,the path of realizing the goal of knowledge and ability.The teaching design discusses the trinity of integration of the-ory and practice,thinking,method and detail with technology application ability supported by theories,teaching guide learning methods,the importance of non-intelligence factor. We puts the above design into teaching practice,receives a good effect.

  8. Discrete mechanics Based on Finite Element Methods

    OpenAIRE

    Chen, Jing-Bo; Guo, Han-Ying; Wu, Ke

    2002-01-01

    Discrete Mechanics based on finite element methods is presented in this paper. We also explore the relationship between this discrete mechanics and Veselov discrete mechanics. High order discretizations are constructed in terms of high order interpolations.

  9. Development of a method for the control of an environmentally friendly cultivation of energy crops at provincial level using a differentiated premium payment; Entwicklung einer Methodik zur Steuerung eines umweltschonenden Energiepflanzenanbaus auf Landesebene durch eine differenzierte Praemienzahlung

    Energy Technology Data Exchange (ETDEWEB)

    Greiff, Kathrin Britta

    2012-07-01

    Facing the global challenge of climate change and finiteness of fossil resources renewable resources are becoming more and more important at present. At the moment, biomass is the most important renewable resource in Germany. The rising cultivation of energy crops, supported by political instruments, has a negative environmental impact on the natural resources soil, water and biodiversity. The existing agricultural legislation can not avoid such negative environmental impact. Thus, it seems reasonable to realign the existing support scheme for energy crop use to a system that operates regional and crop specific but includes the whole agricultural sector as well. Such a newly designed system could be realised by a differentiated premium. This instrument is most suitable to prevent environmental damage due to the advantages of a regional use and a differentiation of crop species. The aim of this work has been to identify an easy applicable and transferable control mechanism for an environmental friendly support scheme of energy crops and also to develop and test a method as basis for this control mechanism. The design of a regional and crop specific premium has been described as a possible political instrument. The method has been developed and tested within a case study for Bavaria, one of Germany's federal states. For the investigation the level of agrarian production regions has been chosen. For each region it has been assessed which crops should and which ones should not be cultivated. For this, the actual environmental risk for the agricultural activity has been calculated by using landscape analysis on the basis of environmental risk assessment. Agrarian statistics (InVeKos) and digital GIS-based site data has been used as data base. With this data base it has been possible to illustrate the environmental risk for the natural resources soil, water and biodiversity in sufficient exactness. The comparison of the periods 1994-96 and 2005-07 approved that the

  10. Comparison and Reference of the Talent Cultivation System in Vocational Education Based on Cultural Creation and Information Industry between Fujian and Taiwan

    Directory of Open Access Journals (Sweden)

    Di Jun An

    2016-01-01

    Full Text Available firstly, the current situation of the cultural creation and information industry in Fujian and Taiwan is investigated and compared in this paper. Secondly, the characteristics and advantages of the talent cultivation system in the cultural creation and information industry of Taiwan are investigated. Finally, the advantages of Taiwanese cultural creation and information industry in talent cultivation are referred to greatly cultivate the talents of cultural creation and information industry in Fujian Province.

  11. [The Theory and Practice of Health Cultivation Qigong Exercise in Traditional Chinese Medicine].

    Science.gov (United States)

    Chang, Mei-Ying

    2015-12-01

    The health cultivation qigong exercise in traditional Chinese medicine refers to a traditional, integrated method of illness prevention and body strengthening, which promotes the functions of qi and the blood, smooths the meridians (energy channels), and balances the viscera and bowels through the regulation of the mind, the breathing, and the body. The concept of using qi to cultivate human life is part of the health cultivation practices of ancient Chinese codes and of Chinese medicine. This concept includes the principles, methods, essences, and clinical applications of the practice. In addition, traditional health cultivation references the concepts of yinyang, viscera and bowels, qi and blood, meridians, and essential energy spirit theory in order to explain the human biological phenomena, the theoretical and practical perspectives of qigong, and the basis of the treatment principle. The health cultivation qigong exercise of Chinese medicine utilizes the concept of the "unity of nature and human beings" in traditional Chinese thinking in its practice, which emphasizes the conformity to nature and seasons. In order to fully leverage the benefits from the purpose of health cultivation in qigong practice, the priority is to understand the health cultivation mechanism, the essentials/matters, and the precautions of qigong practices. Recently, the evidence regarding both the biological and the psychological benefits of qigong practices have been demonstrated in numbers of research articles. In particular, qigong is currently considered to be one of the best mild exercises that is suited to all age groups. Professional nurses are suggested to include the health cultivation qigong exercise as part of activities that target health improvement and illness prevention. Due to the diversity in qigong as practiced by different health cultivation qigong exercise sects, it is essential to accumulate more clinical evidence by conducting greater numbers of rigorous studies

  12. Agent Based Image Segmentation Method : A Review

    OpenAIRE

    Pooja Mishra; Navita Srivastava; Shukla, K. K.; Achintya Singlal

    2011-01-01

    Image segmentation is an important research area in computer vision and many segmentation methods have been proposed. This paper attempts to provide a brief overview of elemental segmentation techniques based on boundary or regional approaches. It focuses mainly on the agent based image segmentation techniques

  13. Capacity for colonization and degradation of horse manure and wheat-straw-based compost by different strains of Agaricus subrufescens during the first two weeks of cultivation.

    Science.gov (United States)

    Farnet, Anne-Marie; Qasemian, Leila; Peter-Valence, Frédérique; Ruaudel, Florence; Savoie, Jean Michel; Ferré, Elisée

    2013-03-01

    The potential of Agaricus subrufescens strains to colonize and transform horse manure and wheat-straw-based mushroom compost under the physico-chemical conditions typically used for Agaricus bisporus was assessed. Lignocellulolytic activities, H2O2 production and substrate transformation (assessed via CP/MAS NMR of (13)C) for certain A. subrufescens strains were similar or even greater than those obtained for an A. bisporus strain used as control. Moreover, the functional diversity of the microbial communities of the substrate was not altered by the growth of A. subrufescens after 2weeks. These findings obtained with mesocosms simulating the incubation phase of the mushroom production process hold promise for the improvement of cultivation of this tropical Agaricus species on European standard mushroom compost.

  14. Influence of carvacrol and 1,8-cineole on cell viability, membrane integrity, and morphology of Aeromonas hydrophila cultivated in a vegetable-based broth.

    Science.gov (United States)

    de Sousa, Jossana Pereira; de Oliveira, Kataryne Árabe Rimá; de Figueiredo, Regina Celia Bressan Queiroz; de Souza, Evandro Leite

    2015-02-01

    This study investigated the effects of carvacrol (CAR) and 1,8-cineole (CIN) alone (at the MIC) or in combination at subinhibitory amounts (both at 1/8 MIC) on the cell viability, membrane permeability, and morphology of Aeromonas hydrophila INCQS 7966 (A. hydrophila) cultivated in a vegetable-based broth. CAR and CIN alone or in combination severely affected the viability of the bacteria and caused dramatic changes in the cell membrane permeability, leading to cell death, as observed by confocal laser microscopy. Scanning and transmission electron microscopy images of bacterial cells exposed to CAR or CIN or the mixture of both compounds revealed severe changes in cell wall structure, rupture of the plasma membrane, shrinking of cells, condensation of cytoplasmic content, leakage of intracellular material, and cell collapse. These findings suggest that CAR and CIN alone or in combination at subinhibitory amounts could be applied to inhibit the growth of A. hydrophila in foods, particularly as sanitizing agents in vegetables.

  15. The Physical Location of Gm-2 and Gm-6 in O.officinalis with BAC-FISH Based on Comparative RFLP Map of Wild Rice and Cultivated Rice

    Institute of Scientific and Technical Information of China (English)

    QIN Rui; WEI Wen-hui; NING Shun-bin; JIN Wei-wei; HE Guang-cun; SONG Yun-chun

    2002-01-01

    Rice BAC library is used widely in rice genome research due to its distinctive advantages over other library systems. In this study, two rice BAC clones closely linked to rice gall midge resistance, Gm-2 and Gm-6, were in situ hybridized to Oryza officinalis chromosomes. They were located on the long arm of chromosome 4 with FL 72.33% and 77.10% respectively and their FL was consistent with the selective marker of rice, RG214 and RZ569. The frequency of signal detection was 61.2% and 59.5% respectively.Our study was based on comparative RFLP map of wild rice, O. officinalis, and cultivated rice, O. sativa.

  16. Effects of Seedling Cultivation Methods on Seedling Cultivation of Major Vegetables%不同育苗方式对主要蔬菜作物育苗效果的影响

    Institute of Scientific and Technical Information of China (English)

    徐海; 宋波; 陈龙正; 卢之道; 袁希汉

    2015-01-01

    Objective] The alm was to expIore the effects of cuItivation methods on vegetabIe growth in seedIing stage. [Method] The test incIuded 4 treatments, as foI-Iows: treatment 1 (T1) where seedIings were cuItivated as per water fiIm-based cuI-tivation and irrigation proceeded reguIarIy; treatment 2 (T2) where irrigation proceed-ed continuousIy as per water fiIm-based cuItivation; treatment 3 (T3) where seedIings were cuItivated as per fIoating nursing; controI treatment (CK) where seedIings were cuItivated as per seedIing-tray cuItivation in order to survey the number of germinated seedIings per tray. [Result] Both of water fiIm-based cuItiva-tion and fIoating nursing took advantages in germination speed and aduIt seedIing rate, compared with tray cuItivation method. [Conclusion] Water fiIm-based cuItivation and fIoating nursing make innovations in water management during seedIing cuItiva-tion, which improve germination speed, aduIt seedIing rate and seedIing quality, making contribution to Iarge-scale production of greenhouse vegetabIes through ap-pIications and practices.%[目的]研究不同育苗方式对主要蔬菜作物苗期生长的影响。[方法]试验设4个处理:水膜育苗,适时间断供水( T1);水膜育苗,连续供水(T2);水浮育苗(T3);普通穴盘育苗(CK)。调查每个育苗盘中出苗株数。[结果]水膜育苗和水浮育苗等新型育苗方式相比普通穴盘育苗在出苗速度、成苗率方面有较明显的优势,更易于培育壮苗。[结论]水膜育苗和水浮育苗等新型育苗技术通过在育苗过程中水分管理方面的创新,一定程度上提高了出苗速度、成苗率和幼苗质量。

  17. Software Testing Method Based on Model Comparison

    Institute of Scientific and Technical Information of China (English)

    XIE Xiao-dong; LU Yan-sheng; MAO Cheng-yin

    2008-01-01

    A model comparison based software testing method (MCST) is proposed. In this method, the requirements and programs of software under test are transformed into the ones in the same form, and described by the same model describe language (MDL).Then, the requirements are transformed into a specification model and the programs into an implementation model. Thus, the elements and structures of the two models are compared, and the differences between them are obtained. Based on the diffrences, a test suite is generated. Different MDLs can be chosen for the software under test. The usages of two classical MDLs in MCST, the equivalence classes model and the extended finite state machine (EFSM) model, are described with example applications. The results show that the test suites generated by MCST are more efficient and smaller than some other testing methods, such as the path-coverage testing method, the object state diagram testing method, etc.

  18. Planting Environment of Imitation Wild Ginseng and a High Efficient Cultivation Techniques by the Artificial Mediated Method%仿山参种植环境与人工介导高效种植技术1)

    Institute of Scientific and Technical Information of China (English)

    孙绍鹏; 顾宇书; 陈红伟

    2013-01-01

      根据山参的生长习性和品质特征,在依据多年从事林下参种植经验和充分调查基础上,总结了仿山参适宜种植环境和人参人工介导仿山参高效种植技术,采用该项技术能够培养出身形和质量与野山参相媲美人参珍品,为培育高质量的仿山参珍品提供可靠的实用技术。%According to the ginseng growth habit and quality characteristics ,the ginseng planting environ-ment of Imitation wild ginseng was summarized by the way of comprehensive investigation .Based on sev-eral years of experience engaged in planting ginseng under forest ,a technolyge which was differente from the current planting ginseng was adopted .In this way ,a kind of manual mediated method for imitation wild ginseng in body shape and quality by the way of plantation was summarized .with the technology we can cultivate the same body shape and similar quality wild ginseng treasures and provide reliable technolo-gy of mimic wild ginseng .

  19. DNA-based taxonomic identification of basidiospores in hallucinogenic mushrooms cultivated in "grow-kits" seized by the police: LC-UV quali-quantitative determination of psilocybin and psilocin.

    Science.gov (United States)

    Gambaro, Veniero; Roda, Gabriella; Visconti, Giacomo Luca; Arnoldi, Sebastiano; Casagni, Eleonora; Dell'Acqua, Lucia; Farè, Fiorenza; Paladino, Eleonora; Rusconi, Chiara; Arioli, Stefania; Mora, Diego

    2016-06-01

    The taxonomic identification of the biological material contained in the hallucinogenic mushrooms culture media, was carried out using a DNA-based approach, thus highlighting the usefulness of this approach in the forensic identification of illegal samples also when they are present as basidiospores mixed in culture media and spore-bearing fruiting body are not present. This approach is very useful as it allows the unequivocal identification of potentially illicit material before the cultivation and it enables to stop the material to the Customs and to destroy it due to its dangerousness without cultivating the "grow-kits" and without instructing a criminal case. In fact, even if psilocin and psilocybin and the whole mushrooms are illegal in many countries, there is no specific indication in the law about the so called "grow-kits", containing the spores. To confirm the data obtained by the taxonomic identification, a simple, reliable, efficient LC-UV method, using tryptamine as internal standard, suitable for the forensic quali-quantitative determination of psilocin and psilocybin in hallucinogenic mushroom was optimized, validated and applied to the mushrooms grown after the cultivation of the grow-kits seized by the judicial authority, with the authorization of the Ministry of Health. A cation exchange column was used in a gradient elution mode (Phase A: 50mMK2HPO4; 100mM NaCl pH=3 Phase B: methanol). The developed method was linear over the calibration range with a R(2)>0.9992 for both the analytes. The detection and quantification limits were respectively 0.01 and 0.1μg/mL for psilocybin and 0.05μg/mL and 0.1μg/mL for psilocin and the intra- and inter-day precision was satisfactory (coefficients of variation <2.0% for both the analytes). The content of psilocybin in the mushrooms grown from the seized "grow-kits" ranged from 1.02 to 7.60mg/g of dry vegetable material, while the content of psilocin from 0.415 to 8.36mg/g. PMID:27021629

  20. DNA-based taxonomic identification of basidiospores in hallucinogenic mushrooms cultivated in "grow-kits" seized by the police: LC-UV quali-quantitative determination of psilocybin and psilocin.

    Science.gov (United States)

    Gambaro, Veniero; Roda, Gabriella; Visconti, Giacomo Luca; Arnoldi, Sebastiano; Casagni, Eleonora; Dell'Acqua, Lucia; Farè, Fiorenza; Paladino, Eleonora; Rusconi, Chiara; Arioli, Stefania; Mora, Diego

    2016-06-01

    The taxonomic identification of the biological material contained in the hallucinogenic mushrooms culture media, was carried out using a DNA-based approach, thus highlighting the usefulness of this approach in the forensic identification of illegal samples also when they are present as basidiospores mixed in culture media and spore-bearing fruiting body are not present. This approach is very useful as it allows the unequivocal identification of potentially illicit material before the cultivation and it enables to stop the material to the Customs and to destroy it due to its dangerousness without cultivating the "grow-kits" and without instructing a criminal case. In fact, even if psilocin and psilocybin and the whole mushrooms are illegal in many countries, there is no specific indication in the law about the so called "grow-kits", containing the spores. To confirm the data obtained by the taxonomic identification, a simple, reliable, efficient LC-UV method, using tryptamine as internal standard, suitable for the forensic quali-quantitative determination of psilocin and psilocybin in hallucinogenic mushroom was optimized, validated and applied to the mushrooms grown after the cultivation of the grow-kits seized by the judicial authority, with the authorization of the Ministry of Health. A cation exchange column was used in a gradient elution mode (Phase A: 50mMK2HPO4; 100mM NaCl pH=3 Phase B: methanol). The developed method was linear over the calibration range with a R(2)>0.9992 for both the analytes. The detection and quantification limits were respectively 0.01 and 0.1μg/mL for psilocybin and 0.05μg/mL and 0.1μg/mL for psilocin and the intra- and inter-day precision was satisfactory (coefficients of variation psilocybin in the mushrooms grown from the seized "grow-kits" ranged from 1.02 to 7.60mg/g of dry vegetable material, while the content of psilocin from 0.415 to 8.36mg/g.

  1. Design for the Cultivation of Employment Competence of Higher Vocational Students Based on the Competency%基于胜任特征的高职生就业能力培养设计*

    Institute of Scientific and Technical Information of China (English)

    王小明

    2013-01-01

    本文介绍了基于高职生成功就业胜任特征模型的高职生就业能力培养设计的研究,提出胜任特征水平测试、鉴定胜任特征差距、规划胜任特征差距、自我指导的行动学习、评估行动学习结果等五个环节的基于成功就业胜任特征的自我指导的行动学习法,列举基于胜任特征就业能力培养的需求分析所需胜任特征目标和评价标准。然后提出了基于胜任特征高职生就业能力培养时应当注意的一些问题和思考。%This paper introduces the research of cultivation of employment competency of higher vocational students based on competency model, and proposes self-instructed action learning methods, which are composed of five links:the competency level test, identification of the competency gap, planning the competency gap, self-instructed action learning and evaluation of action learning re-sults. And it also lists the target and evaluation standard of competency based on requirement analysis. At last, it puts forward some problems and reflections on cultivation of employment competency.

  2. Cultivation-Based and Molecular Assessment of Bacterial Diversity in the Rhizosheath of Wheat under Different Crop Rotations.

    Directory of Open Access Journals (Sweden)

    Muhammad Tahir

    Full Text Available A field study was conducted to compare the formationand bacterial communities of rhizosheaths of wheat grown under wheat-cotton and wheat-rice rotation and to study the effects of bacterial inoculation on plant growth. Inoculation of Azospirillum sp. WS-1 and Bacillus sp. T-34 to wheat plants increased root length, root and shoot dry weight and dry weight of rhizosheathsoil when compared to non-inoculated control plants, and under both crop rotations. Comparing both crop rotations, root length, root and shoot dry weight and dry weight of soil attached with roots were higher under wheat-cotton rotation. Organic acids (citric acid, malic acid, acetic acid and oxalic acid were detected in rhizosheaths from both rotations, with malic acid being most abundant with 24.8±2 and 21.3±1.5 μg g(-1 dry soil in wheat-cotton and wheat-rice rotation, respectively. Two sugars (sucrose, glucose were detected in wheat rhizosheath under both rotations, with highest concentrations of sucrose (4.08±0.5 μg g(-1 and 7.36±1.0 μg g(-1 and glucose (3.12±0.5 μg g(-1 and 3.01± μg g(-1 being detected in rhizosheaths of non-inoculated control plants under both rotations. Diversity of rhizosheath-associated bacteria was evaluated by cultivation, as well as by 454-pyrosequencing of PCR-tagged 16S rRNA gene amplicons. A total of 14 and 12 bacterial isolates predominantly belonging to the genera Arthrobacter, Azospirillum, Bacillus, Enterobacter and Pseudomonaswere obtained from the rhizosheath of wheat grown under wheat-cotton and wheat-rice rotation, respectively. Analysis of pyrosequencing data revealed Proteobacteria, Bacteriodetes and Verrucomicrobia as the most abundant phyla in wheat-rice rotation, whereas Actinobacteria, Firmicutes, Chloroflexi, Acidobacteria, Planctomycetes and Cyanobacteria were predominant in wheat-cotton rotation. From a total of 46,971 sequences, 10.9% showed ≥97% similarity with 16S rRNA genes of 32 genera previously shown to include

  3. Cultivation-Based and Molecular Assessment of Bacterial Diversity in the Rhizosheath of Wheat under Different Crop Rotations.

    Science.gov (United States)

    Tahir, Muhammad; Mirza, M Sajjad; Hameed, Sohail; Dimitrov, Mauricio R; Smidt, Hauke

    2015-01-01

    A field study was conducted to compare the formationand bacterial communities of rhizosheaths of wheat grown under wheat-cotton and wheat-rice rotation and to study the effects of bacterial inoculation on plant growth. Inoculation of Azospirillum sp. WS-1 and Bacillus sp. T-34 to wheat plants increased root length, root and shoot dry weight and dry weight of rhizosheathsoil when compared to non-inoculated control plants, and under both crop rotations. Comparing both crop rotations, root length, root and shoot dry weight and dry weight of soil attached with roots were higher under wheat-cotton rotation. Organic acids (citric acid, malic acid, acetic acid and oxalic acid) were detected in rhizosheaths from both rotations, with malic acid being most abundant with 24.8±2 and 21.3±1.5 μg g(-1) dry soil in wheat-cotton and wheat-rice rotation, respectively. Two sugars (sucrose, glucose) were detected in wheat rhizosheath under both rotations, with highest concentrations of sucrose (4.08±0.5 μg g(-1) and 7.36±1.0 μg g(-1)) and glucose (3.12±0.5 μg g(-1) and 3.01± μg g(-1)) being detected in rhizosheaths of non-inoculated control plants under both rotations. Diversity of rhizosheath-associated bacteria was evaluated by cultivation, as well as by 454-pyrosequencing of PCR-tagged 16S rRNA gene amplicons. A total of 14 and 12 bacterial isolates predominantly belonging to the genera Arthrobacter, Azospirillum, Bacillus, Enterobacter and Pseudomonaswere obtained from the rhizosheath of wheat grown under wheat-cotton and wheat-rice rotation, respectively. Analysis of pyrosequencing data revealed Proteobacteria, Bacteriodetes and Verrucomicrobia as the most abundant phyla in wheat-rice rotation, whereas Actinobacteria, Firmicutes, Chloroflexi, Acidobacteria, Planctomycetes and Cyanobacteria were predominant in wheat-cotton rotation. From a total of 46,971 sequences, 10.9% showed ≥97% similarity with 16S rRNA genes of 32 genera previously shown to include isolates

  4. Molecular- and cultivation-based analyses of microbial communities in oil field water and in microcosms amended with nitrate to control H{sub 2}S production

    Energy Technology Data Exchange (ETDEWEB)

    Kumaraswamy, Raji; Ebert, Sara; Fedorak, Phillip M.; Foght, Julia M. [Alberta Univ., Edmonton, AB (Canada). Biological Sciences; Gray, Murray R. [Alberta Univ., Edmonton, AB (Canada). Chemical and Materials Engineering

    2011-03-15

    Nitrate injection into oil fields is an alternative to biocide addition for controlling sulfide production ('souring') caused by sulfate-reducing bacteria (SRB). This study examined the suitability of several cultivation-dependent and cultivation-independent methods to assess potential microbial activities (sulfidogenesis and nitrate reduction) and the impact of nitrate amendment on oil field microbiota. Microcosms containing produced waters from two Western Canadian oil fields exhibited sulfidogenesis that was inhibited by nitrate amendment. Most probable number (MPN) and fluorescent in situ hybridization (FISH) analyses of uncultivated produced waters showed low cell numbers ({<=}10{sup 3} MPN/ml) dominated by SRB (>95% relative abundance). MPN analysis also detected nitrate-reducing sulfide-oxidizing bacteria (NRSOB) and heterotrophic nitrate-reducing bacteria (HNRB) at numbers too low to be detected by FISH or denaturing gradient gel electrophoresis (DGGE). In microcosms containing produced water fortified with sulfate, near-stoichiometric concentrations of sulfide were produced. FISH analyses of the microcosms after 55 days of incubation revealed that Gammaproteobacteria increased from undetectable levels to 5-20% abundance, resulting in a decreased proportion of Deltaproteobacteria (50-60% abundance). DGGE analysis confirmed the presence of Delta- and Gammaproteobacteria and also detected Bacteroidetes. When sulfate-fortified produced waters were amended with nitrate, sulfidogenesis was inhibited and Deltaproteobacteria decreased to levels undetectable by FISH, with a concomitant increase in Gammaproteobacteria from below detection to 50-60% abundance. DGGE analysis of these microcosms yielded sequences of Gamma- and Epsilonproteobacteria related to presumptive HNRB and NRSOB (Halomonas, Marinobacterium, Marinobacter, Pseudomonas and Arcobacter), thus supporting chemical data indicating that nitrate-reducing bacteria out-compete SRB when nitrate is

  5. Human mesenchymal stem cell expression program upon extended ex-vivo cultivation, as revealed by 2-DE-based quantitative proteomics.

    Directory of Open Access Journals (Sweden)

    Andreia Madeira

    Full Text Available Human mesenchymal stem cells (MSC have been on the focus of intense clinical-oriented research due to their multilineage differentiation potential and immunomodulatory properties. However, to reach the clinically meaningful cell numbers for cellular therapy and tissue engineering applications, MSC ex-vivo expansion is mandatory but sequential cell passaging results in loss of proliferative, clonogenic and differentiation potential. To get clues into the molecular mechanisms underlying cellular senescence resulting from extended ex-vivo cultivation of bone marrow (BM MSC, we explored a two-dimensional gel electrophoresis (2-DE based quantitative proteomics to compare the expression programs of Passage 3 cells (P3, commonly used in clinical studies with expanded MSC, and Passage 7 (P7 cells, which already demonstrated significant signs of culture-induced senescence. Proteins of the functional categories "Structural components and cellular cytoskeleton" and "Folding and stress response proteins" are less abundant in P7 cells, compared to P3, while proteins involved in "Energy metabolism", "Cell cycle regulation and aging" and "Apoptosis" are more abundant. The large number of multiple size and charge isoforms with an altered content that were identified in this study in P7 versus P3, namely the cytoskeleton components β-actin (7 forms and vimentin (24 forms, also emphasizes the importance of post-transcriptional modification upon long-term cultivation. The differential protein expression registered suggests that cellular senescence occurring during ex-vivo expansion of BM MSC is associated with the impairment of cytoskeleton remodeling and/or organization and the repair of damaged proteins resulting from cell exposure to culture stress. The genome-wide expression approach used in this study has proven useful for getting mechanistic insights into the observed decrease on the proliferative and clonogenic potential of P7 versus P3 cells and paves the

  6. Human mesenchymal stem cell expression program upon extended ex-vivo cultivation, as revealed by 2-DE-based quantitative proteomics.

    Science.gov (United States)

    Madeira, Andreia; da Silva, Cláudia L; dos Santos, Francisco; Camafeita, Emilio; Cabral, Joaquim M S; Sá-Correia, Isabel

    2012-01-01

    Human mesenchymal stem cells (MSC) have been on the focus of intense clinical-oriented research due to their multilineage differentiation potential and immunomodulatory properties. However, to reach the clinically meaningful cell numbers for cellular therapy and tissue engineering applications, MSC ex-vivo expansion is mandatory but sequential cell passaging results in loss of proliferative, clonogenic and differentiation potential. To get clues into the molecular mechanisms underlying cellular senescence resulting from extended ex-vivo cultivation of bone marrow (BM) MSC, we explored a two-dimensional gel electrophoresis (2-DE) based quantitative proteomics to compare the expression programs of Passage 3 cells (P3), commonly used in clinical studies with expanded MSC, and Passage 7 (P7) cells, which already demonstrated significant signs of culture-induced senescence. Proteins of the functional categories "Structural components and cellular cytoskeleton" and "Folding and stress response proteins" are less abundant in P7 cells, compared to P3, while proteins involved in "Energy metabolism", "Cell cycle regulation and aging" and "Apoptosis" are more abundant. The large number of multiple size and charge isoforms with an altered content that were identified in this study in P7 versus P3, namely the cytoskeleton components β-actin (7 forms) and vimentin (24 forms), also emphasizes the importance of post-transcriptional modification upon long-term cultivation. The differential protein expression registered suggests that cellular senescence occurring during ex-vivo expansion of BM MSC is associated with the impairment of cytoskeleton remodeling and/or organization and the repair of damaged proteins resulting from cell exposure to culture stress. The genome-wide expression approach used in this study has proven useful for getting mechanistic insights into the observed decrease on the proliferative and clonogenic potential of P7 versus P3 cells and paves the way to set

  7. A Property Restriction Based Knowledge Merging Method

    Science.gov (United States)

    Che, Haiyan; Chen, Wei; Feng, Tie; Zhang, Jiachen

    Merging new instance knowledge extracted from the Web according to certain domain ontology into the knowledge base (KB for short) is essential for the knowledge management and should be processed carefully, since this may introduce redundant or contradictory knowledge, and the quality of the knowledge in the KB, which is very important for a knowledge-based system to provide users high quality services, will suffer from such "bad" knowledge. Advocates a property restriction based knowledge merging method, it can identify the equivalent instances, redundant or contradictory knowledge according to the property restrictions defined in the domain ontology and can consolidate the knowledge about equivalent instances and discard the redundancy and conflict to keep the KB compact and consistent. This knowledge merging method has been used in a semantic-based search engine project: CRAB and the effect is satisfactory.

  8. Cloud Based Development Issues: A Methodical Analysis

    Directory of Open Access Journals (Sweden)

    Sukhpal Singh

    2012-11-01

    Full Text Available Cloud based development is a challenging task for various software engineering projects, especifically for those which demand extraordinary quality, reusability and security along with general architecture. In this paper we present a report on a methodical analysis of cloud based development problems published in major computer science and software engineering journals and conferences organized by various researchers. Research papers were collected from different scholarly databases using search engines within a particular period of time. A total of 89 research papers were analyzed in this methodical study and we categorized into four classes according to the problems addressed by them. The majority of the research papers focused on quality (24 papers associated with cloud based development and 16 papers focused on analysis and design. By considering the areas focused by existing authors and their gaps, untouched areas of cloud based development can be discovered for future research works.

  9. 区域耕地粮食生产保障能力及其风险评价方法%Risk assessment method of grain production guarantee capacity of regional cultivated land

    Institute of Scientific and Technical Information of China (English)

    季翔; 刘黎明; 起晓星

    2014-01-01

    区域耕地粮食生产保障能力是区域耕地对综合食物安全所需粮食的保障程度,受区域耕地资源和社会经济等方面的影响。科学评价区域耕地的粮食生产保障能力及其存在风险,对于解决综合食物安全问题起着关键性的作用。该文以区域耕地的粮食供给能力和粮食生产安全量预测为基础,建立了区域耕地粮食生产保障风险的评价方法,并以洞庭湖区为例开展了以县(市、区)为基本单元的实证研究。结果表明:洞庭湖区耕地资源对全国综合食物安全的保障情况较好,但部分县(市、区)的风险等级较高,且临湖区域的风险较相对远离洞庭湖区域的风险高,需要提高粮食生产水平并控制耕地非农化。该文所提出的区域耕地粮食生产保障能力及其风险评价方法为研究和评估不同区域粮食安全状况与耕地资源之间的相互关系提供参考。%Food security always attracts strong attention from researchers and policy makers in China, because it has more than one fifth of the world’s population, but the total cultivated area accounts for only about seven percent of the world’s total cultivated area. However, relevant studies were normally conducted at the national level, which generally considered China’s food security as a whole or used a single index system set for analysis. Therefore, study on food security from a regional scale will be of great value for policy makers. Grain production guarantee capacity of regional cultivated land is the satisfaction degree of regional grain supply to meet the national grain demand, which is mainly influenced by local cultivated land resources, cropping systems, and socio-economic factors. Regional total grain demand should be measured from a national perspective because of the mobility of grain and population;more specifically, it should be calculated based on the proportion of regional resource endowments

  10. Method for a human based design process

    OpenAIRE

    Van Herck, Tine

    2013-01-01

    We are developing a method for a human based design process. This means a design method that enables to improve the social relevance of projects. It can be applied to projects of different scale : from interior architecture to landscape strategies. What should be the attitude of a designer in today's society where all certainties threaten to disappear? How to contribute something small to society in order to create a stronger tissue, a safety net, a solid found...

  11. Recommendation advertising method based on behavior retargeting

    Science.gov (United States)

    Zhao, Yao; YIN, Xin-Chun; CHEN, Zhi-Min

    2011-10-01

    Online advertising has become an important business in e-commerce. Ad recommended algorithms are the most critical part in recommendation systems. We propose a recommendation advertising method based on behavior retargeting which can avoid leakage click of advertising due to objective reasons and can observe the changes of the user's interest in time. Experiments show that our new method can have a significant effect and can be further to apply to online system.

  12. Resposta do milho a fontes e modos de aplicação de fósforo durante três cultivos sucessivos em solo da região do Cerrado Maize response to phosphorus sources and application methods during three successive cultivations in soil of the Cerrado region

    Directory of Open Access Journals (Sweden)

    Álvaro Vilela de Resende

    2006-06-01

    Full Text Available Objetivou-se comparar fertilizantes fosfatados em diferentes modos de aplicação, durante três cultivos sucessivos de milho, num Argissolo Vermelho já adubado anteriormente. Foi utilizado um fatorial 4 x 2+1, envolvendo quatro fontes de P (superfosfato triplo ST, termofosfato magnesiano TM, fosfato reativo de Arad FR e fosfato natural de Araxá FA, duas formas de aplicação (a lanço ou no sulco de plantio, e uma testemunha (sem P como tratamento adicional. Foram fornecidos 180 kg ha-1 de P2O5 no primeiro cultivo, com base nos teores totais das fontes. Para os cultivos seguintes, não foi feito preparo do solo. Determinaram-se os teores de nutrientes no solo e nas folhas de milho e a produção de grãos. As respostas aos tratamentos foram mais discrepantes inicialmente e tenderam à eqüidade com os cultivos sucessivos. Nas duas primeiras safras, as fontes de maior solubilidade (ST e TM ocasionaram as maiores produções. Os fosfatos naturais (FR e FA apresentaram aumento de eficiência com o tempo. A aplicação localizada do FR proporcionou alta produtividade na terceira safra. O residual de antigas adubações e as condições climáticas influenciaram os efeitos dos tratamentos.The objective of this study was to compare phosphate fertilizers in different methods of application during three successive maize cultivations in a Red Argisol (Hapludults which had been previously fertilized. Treatments arranged in a 4x2+1 factorial design combined four P sources in the amount of 180 kg ha-1 P2O5 (triple superphosphate - TS, magnesium termophosphate - MT, Arad reactive rock phosphate - RP, and Araxá rock phosphate - AP, two application methods (broadcasted or banded in the seeding furrow, and a check plot (without P as an additional treatment. The applied P ratio was based on the total P2O5 content of each fertilizer. After the first cultivation, the soil was no longer revolved. Soil and leaf nutrient concentrations, and the grain yield were

  13. 浅谈培养学生音乐记忆力的方法与途径%A Brief Discussion on the Methods and Approaches of Cultivating Students' Music Memory

    Institute of Scientific and Technical Information of China (English)

    张建芬

    2013-01-01

    音乐记忆力指的是对音乐作品的记忆能力,也是我们学生学习音乐必须具备的基本能力,正确的音乐记忆应遵循记忆的规律,讲究方法,在学习中加以培养。本文旨在如何培养学生良好的音乐记忆能力上做了一些尝试。%Music memory, students' basic ability in learning mu-sic, is the memorizing ability of music works. A correct music memory obeys the rules and methods of memory and should be cultivated in learning. This paper attempts to cultivate students' correct music memory ability.

  14. Oil monitoring methods based on information theory

    Institute of Scientific and Technical Information of China (English)

    XIA Yan-chun; HUO Hua

    2009-01-01

    To evaluate the Wear condition of machines accurately,oil spectrographic entropy,mutual information and ICA analysis methods based on information theory are presented.A full-scale diagnosis utilizing all channels of spectrographic analysis can be obtained.By measuring the complexity and correlativity,the characteristics of wear condition of machines can be shown clearly.The diagnostic quality is improved.The analysis processes of these monitoring methods are given through the explanation of examples.The availability of these methods is validated and further research fields are demonstrated.

  15. Rough set-based feature selection method

    Institute of Scientific and Technical Information of China (English)

    ZHAN Yanmei; ZENG Xiangyang; SUN Jincai

    2005-01-01

    A new feature selection method is proposed based on the discern matrix in rough set in this paper. The main idea of this method is that the most effective feature, if used for classification, can distinguish the most number of samples belonging to different classes. Experiments are performed using this method to select relevant features for artificial datasets and real-world datasets. Results show that the selection method proposed can correctly select all the relevant features of artificial datasets and drastically reduce the number of features at the same time. In addition, when this method is used for the selection of classification features of real-world underwater targets,the number of classification features after selection drops to 20% of the original feature set, and the classification accuracy increases about 6% using dataset after feature selection.

  16. 土地利用现状调查资料在耕地地力评价中的应用%Application research of cultivated land fertility evaluation based on land use status data

    Institute of Scientific and Technical Information of China (English)

    蒋玉根; 吴豪翔; 裘希雅; 白金河; 何旭华; 陈侃

    2011-01-01

    以1∶10 000土地利用现状图为空间数据框架基础,结合农业部门现有的土壤普查和样点调查分析资料进行耕地地力评价,相比目前我国测土配方施肥工作方案中以1∶50 000为基础进行的耕地地力评价结果,本研究方法的评价结果耕地分布的空间定位更正确、更切合现状,评价单元的划分更合理、耕地地力等级划分更加精细,能大幅度提高耕地地力评价的精度.本研究方法在耕地地力评价上的应用,有利于为建立测土配方施肥信息管理系统提供详细的基础信息和科学管理耕地,更好地满足精准农业和农业生产中施肥管理对耕地信息的需要.%The purpose of this paper was to evaluate cultivated land fertility based on land use status map at 1- 10 000 scale combined with existing soil survey and soil sample analysis data. The evaluation results showed that spatial positioning of cultivated land distribution was a more accurate and practical situation and that the assessment unit division of cultivated land classification was more reasonable and more sophisticated, and the evaluation accuracy was greatly improved, compared to evaluation at 1:50 000 scale implemented in the work program of testing soil for formulated fertilization in our country. The research method in this paper was beneficial for the establishment of soil testing and formulated fertilization information management systems to provide detailed basic information and scientific management of farmland. It can better meet farmland information needs on precision agriculture and fertilizer management for agricultural production.

  17. Cultivated Land Changes and Their Driving Forces-A Satellite Remote Sensing Analysis in the Yellow River Delta, China

    Institute of Scientific and Technical Information of China (English)

    ZHAO Geng-Xing; G.LIN; J.J.FLETCHER; C.YUILL

    2004-01-01

    Taking Kenli County in the Yellow River Delta, China, as the study area and using digital satellite remote sensing techniques, cultivated land use changes and their corresponding driving forces were explored in this study. An interactive interpretation and a manual modification procedure were carried out to acquire cultivated land information. An overlay method based on classification results and a visual change detection method which was supported by land use maps were employed to detect the cultivated land changes. Based on the changes that were revealed and a spatial analysis between cultivated land use and related natural and socio-economic factors, the driving forces for cultivated land use changes in the study area were determined.The results showed a decrease in cultivated land in Kenli County of 5321.8 ha from 1987 to 1998, i.e.,an average annual decrement of 483.8 ha, which occurred mainly in the central paddy field region and the northeast dry land region. Adverse human activities, soil salinization and water deficiencies were the driving forces that caused these cultivated land use changes.

  18. Spatial variability of available phosphorus for cultivated soil based on GARBF neural network%基于GARBF神经网络的耕地土壤有效磷空间变异分析

    Institute of Scientific and Technical Information of China (English)

    徐剑波; 宋立生; 夏振; 张桥; 胡月明

    2012-01-01

    correlation in surface cultivated soil AP was weak in the five sampling scales (training sample points were 100, 200, 300,400 and 500), while unobvious in wide range. Predictions of soil AP in simulation using GARBF neural network was better than that using radial basis function(RBF) neural network (Near-RBF) prediction model based on several closest neighbors and Ordinary Kriging method. In practical application, the spatial interpolation map by GARBF neural network method with 300 soil samples showed a serious trend of surplus phosphate in cropping in Gaozhou city. Diffusive surplus phosphorus made a serious threat to the water environment in this region. The results provide a theoretical basis and technical support for predicting soil property spatial distribution accurately, using fertilizer properly and mitigating non-point source pollution of water.

  19. 袁同礼的图书馆人才培植方法及启示%Yuan Tongli's Methods about Cultivating Talents of Libraries and Its Revelation

    Institute of Scientific and Technical Information of China (English)

    潘梅

    2011-01-01

    Yuan Tongli who is an important leader of Chinese librarianship in the period of the Republic of China, he has own unique ways of cultivating talents. In selecting talents, he attached importance to knowledge basis and academic skills of talents. In using talents, he respected talents' interests and took advantage of their strengths. In cultivating talents, he provided many opportunities of academic training and advanced studying for talents. In retaining talents, he cared them and fully played leadership charisma. Yuan Tongli's methods about cultivating talents have a great inspiration for libraries today. Firstly, cultivating talents of library is a strategic issue related to librarianship development and academic progress. Secondly, libraries should moderately increase the space-level positions in favor to cultivate talents. Finally, libraries should pay attention to the establishment of long-term mechanism of cultivating talents.%民国时期中国图书馆事业的重要领导者袁同礼在培植图书馆人才上有自己独特的方法:在选才方面,重视人才的知识基础和学术功底;在用才方面,尊重人才的志趣,用人所长;在育才方面,努力为人才提供学术训练和进修深造的机会,注重人才的成长;在留才方面,关心人才,充分发挥个人的领导魅力。此方法对当今图书馆的人才培植仍有极大的启发意义。图书馆人才培养是事关图书馆事业发展、学术进步的战略问题,图书馆应适度增加空间职位层次,建立长效的人才培养机制,为人才提供多方面的成长渠道。

  20. Exploration on the Cultivation of Gray Collar Talents Based on the Perspective of Internal Career%基于内职业生涯角度探讨灰领人才的培养

    Institute of Scientific and Technical Information of China (English)

    陈伟

    2015-01-01

    Currently, there is a vicious cycle of talent supply in the job market, that is,"students have difficulties in employment and enterprises have difficulties in recruitment", and gray collar, as a new career field, will be the key to solving the paradox be-tween university students' employment problems and China's tal-ent supply. Starting from the grim employment situation of uni-versity students and the shortage of gray collar talents, this paper analyzes university students' abandonment of "gray collar" and dilemmas in employment due to their obsolete outlooks on em-ployment, and based on the perspective of internal career, finds methods to establish university students' new outlooks on em-ployment and cultivate gray collar talents, thus arousing the pon-dering on the environment for gray collar cultivation in China.%当前,我国就业市场上存在着“学生就业难、企业招工难”的人才供给的怪圈,而灰领作为一种新的职业领域,将成为解决大学生就业问题和我国人才供给悖论的关键所在。本文从我国大学生就业的严峻形势和灰领人才的紧缺问题着手,对大学生因陈旧就业观而放弃“灰领”、就业两难的成因进行分析,从而基于内职业生涯的角度,找出树立大学生新型就业观、打造灰领人才的方法,同时,引出高校对我国灰领培育环境的思考。

  1. Research on Talent Cultivation Objectives for College Physical Education Majors Based on Manage-ment By Objectives%基于“目标管理理论”的高师体育教育专业人才培养目标管理探析

    Institute of Scientific and Technical Information of China (English)

    沈强

    2014-01-01

    通过文献资料法、逻辑分析法,对我国高师体育教育专业人才培养目标进行了历史梳理和现状分析,结果认为:目前高师体育教育专业人才培养现状存在:人才就业导向现状与高师院校体育教育专业目标的不适应、人才培养的课程设置与高师院校体育教育专业目标的不适应、个人发展目标模糊与高师院校体育教育专业目标的不适应等问题。根据“目标管理理论”与存在的问题,提出了高师体育教育专业人才培养目标管理体系,并从理论依据、体系构建、实施路径、管理策略等方面对人才培养目标管理体系做了具体分析,为高师体育教育专业人才培养管理提供参考依据。%With the methods of documentary information and logic analysis ,based on the historical introduction and the analysis of present situation about talent cultivation objective for college physical education majors ,the survey result suggests that at present , the problems in talent cultivation objective for college physical education majors lie in following aspects :talent cultivation objective can not adapt itself to employment direction ;the curriculum can not agree with talent cultivation objectives ;vague personal develop-ment goals can not agree with talent cultivation objectives .According to Management By Objectives and existing problems ,the paper puts forwards talent cultivation objective management system for college physical education majors ,and analyzes the management system from aspects of theory basis ,system construction ,implement approach ,and management policy ,which provides some refer-ences for cultivating talents of college physical education majors .

  2. A Methodological Investigation of Cultivation.

    Science.gov (United States)

    Rubin, Alan M.; And Others

    Cultivation theory states that television engenders negative emotions in heavy viewers. Noting that cultivation methodology contains an apparent response bias, a study examined relationships between television exposure and positive restatements of cultivation concepts and tested a more instrumental media uses and effects model. Cultivation was…

  3. A study on the comparison of antioxidant effects among cultivated ginseng, and cultivated wild ginseng extracts -Using the measurement of superoxide and hydroxy radical scavenging activities-

    Directory of Open Access Journals (Sweden)

    Tae Jin, Rhim

    2009-06-01

    Full Text Available Objectives : The objective of this study was to compare the antioxidant effects among cultivated wild ginseng and ginseng extracts. Methods : In vitro antioxidant activities were examined by superoxide and hydroxyl radical scavenging activities of ginseng and cultivated wild ginseng extracts. Results : 1. In the superoxide radical scavenging activities of ginseng and cultivated wild ginseng extracts, antioxidant activities of cultivated wild ginseng extracts was showed higher than cultivated ginseng in the concentration of 0.25 and 0.50㎎/㎖. 2. In the hydroxyl radical scavenging activities of ginseng and cultivated wild ginseng extracts, antioxidant activities of cultivated wild ginseng extracts was showed higher than cultivated ginseng in the concentration of 1.0, 2.5, and 5.0㎎/㎖. Conclusions : In summary, the results of this study demonstrate that cultivated wild ginseng extracts had higher antioxidant activities to cultivated ginseng.

  4. 基于校企双主体的创意人才培养模式研究%Research on Creative Talents Cultivation Mode Based on Double Subjects of College and Enterprise

    Institute of Scientific and Technical Information of China (English)

    曾文英; 曾东海; 吴积军

    2013-01-01

    Based on the contents of double subjects of college and enterprise, this paper describes the strategy of creative talents to be cultivated, multiple cooperative methods between college and enterprise and talents cultivation mode. The definition of creative talents, combination mode of double subjects between college and enterprise, and the characteristics and paths of creative talents training have been analyzed and discussed. The creative talents training mode based on double subjects of college and enterprise is proposed and the mode can take use of the both resources, with interactive, multiple dimensions expansion, dynamic process, flexible and available, and etc. Some innovative thought and ideas have been proposed, and the category based on cooperation methods have been divided into dynamic state and constant state, the implementation methods have been divided into micro mode, middle mode and macro mode. The development modes contain wide development, deep development and net development.%  通过对校企双主体的内涵为出发点,论述了创意人才培养的策略、校企合作的多种方式构建的人才培养模式。分别对创意人才定义、校企双主体结合模式、以及基于模式的创意人才培养的特点与途径等进行了分析和探讨。提出的基于校企双主体的创意人才培养模式具有综合利用校企资源、全面互动、多维拓展、动态实施、灵活可行等的特点。对于创意人才培养模式提出了一些创新思路和创新理念,并对模式中的协作方式划分为动态和常态,将模式的实现方式分为微模式、中模式、宏模式,将拓展方式归结为横向拓展、纵向拓展和网状拓展。

  5. Bayesian individualization via sampling-based methods.

    Science.gov (United States)

    Wakefield, J

    1996-02-01

    We consider the situation where we wish to adjust the dosage regimen of a patient based on (in general) sparse concentration measurements taken on-line. A Bayesian decision theory approach is taken which requires the specification of an appropriate prior distribution and loss function. A simple method for obtaining samples from the posterior distribution of the pharmacokinetic parameters of the patient is described. In general, these samples are used to obtain a Monte Carlo estimate of the expected loss which is then minimized with respect to the dosage regimen. Some special cases which yield analytic solutions are described. When the prior distribution is based on a population analysis then a method of accounting for the uncertainty in the population parameters is described. Two simulation studies showing how the methods work in practice are presented. PMID:8827585

  6. A Space Flight Cultivation Protocol for Arabidopsis

    Science.gov (United States)

    Levine, H. G.

    2008-06-01

    A tube-based method is presented for the cultivation and manipulation of Arabidopsis thaliana during space flight experimentation. Seeds were germinated on rock-wool plugs and subsequently transferred into modified polypropylene conical tubes (cut to 5 cm lengths) at 7 days after planting. Each tube contained four side-situated slits through which capillary mat strips were woven. An additional capillary mat wick extended from below the tube up through the bottom to the mid-interior portion. The incorporation of Fibrous Ion Exchange Resin Substrate provided nutrients. The tubes were transferred to plant compartments containing a horticulture foam matrix that received water inputs. Vigorous seedling development through to seed production was achieved. Dispersed seeds frequently germinated on top of the foam substrate, yielding a 2nd generation of seedlings. The methods used herein could be applied to other plant species to be flown in space.

  7. Nataf transformation based point estimate method

    Institute of Scientific and Technical Information of China (English)

    LI HongShuang; L(U) ZhenZhou; YUAN XiuKai

    2008-01-01

    Structural probabilistic analysis quantifies the effect of input random variables, such as material proper-ties, geometrical parameters and loading conditions, on the structural responses. The point estimate method (PEM) is a direct and easy-used way to perform the structural probabilistic analysis in practice. In this paper, a novel and efficient point estimate method is proposed for computing the first four statistical moments of structural response which is a function of input random variables. The method adopts Nataf transformation to replace Rosenblatt transformation in conventional point estimate method. Because of the nature of engineering problems and limited statistical data, the joint probability density function (PDF) of all input random variables is hard to acquire, but it must be known in Rosenblatt transformation. A more common case is that the marginal PDF of each random variable and the correlation matrix are available, which just satisfy the service condition of Nataf transformation. Hence the Nataf transformation based point estimate method is particularly suitable for engineering applications. The comparison be-tween the proposed method and the conventional point estimate method shows that (1) they are equiva-lent when all random variables are mutually independent; (2) if the marginal PDFs and the correlation matrix are known, the conventional PEM cannot be applicable, but the proposed method can give a ra-tional approximation. Finally, the procedure is demonstrated in detail through a simple illustration.

  8. Spatial distribution of prime farmland based on cultivated land quality comprehensive evaluation at county scale%基于耕地质量综合评价的县域基本农田空间布局

    Institute of Scientific and Technical Information of China (English)

    奉婷; 张凤荣; 李灿; 曲衍波; 朱凤凯

    2014-01-01

    For the cultivated land quality comprehensive evaluation there have been relatively abundant research results at present, but most of them tend to judge the suitability of spatial distribution of cultivated land and prime farmland without taking sufficient attention to the ecological security. The cultivated land quality comprehensive evaluation plays an important part as prerequisites and groundwork in demarcating of prime farmland and high-standard prime farmland construction. On the basis of dissecting the concept and method of cultivated land quality evaluation, the paper constructed a cultivated quality comprehensive evaluation system by way of selecting relevant indicators from the natural quality, site conditions, spatial pattern and ecological security of cultivated land through referring to agricultural land classification, agricultural land gradation, cultivated land scale operation and its ecological security. The study aimed at meeting the inner demands for demarcating of prime farmland and made up for the deficiency in the present related researches, then achieved quantitative analysis of various indexes and calculation on the cultivated land quality comprehensive index with the help of GIS. On the above basis, the study divided 5 916 cultivated land parcels into 4 different types according to the similarity theory in Pinggu district:1) The first one, named as the prior planning type, was up to 4 325.29 hm2 and accounted for 35.06% of regional total cultivated land area, distributed mainly in the western, southern and southwest plains of Pinggu district, mainly in the town of Donggaocun, followed by the towns of Yukou and Mafang, which showed comprehensive optimum in the natural quality, site conditions, ecological security and spatial pattern of cultivated land as the preferred object to be prime farmland or high-standard prime farmland. This type became the core part of cultivated land protected in Pinggu district. 2) The second one, named as the

  9. The Reform of "Web Design"Course Based on Professional Competence Cultivation%基于职业能力培养的高职《网页设计》课程改革

    Institute of Scientific and Technical Information of China (English)

    胡芳芳

    2013-01-01

    基于职业能力视角对高职网页设计课程进行改革,主要从分析高职学生职业能力培养要求和现在课程教学存在的问题入手,探索了基于职业能力培养的课程改革思路,从教学内容、教学方法、考核方式等方面提出了具体的改革实施方案。%The reform of“Web Design”course based on professional competence perspective. It mainly explores the course re?form idea based on professional competence cultivation from the analysis of students' professional capabilities and problems in cur?rent course teaching . And we put forward specific reform implementation scheme from the aspects of teaching content, teaching methods , assessment methods.

  10. Soilless cultivation system for functional food crops

    International Nuclear Information System (INIS)

    This soilless cultivation system is based on the fertigation system and cultivation technologies using Functional Plant Cultivation System (FPCS). EBARA Japan has been studying on the cultivation conditions in order to enhance the function of decease risk reduction in plants. Through the research and development activities, EBARA found the possibilities on the enhancement of functions. Quality and quantity of the products in term of bioactive compounds present in the plants may be affected by unforeseen environmental conditions, such as temperature, strong light and UV radiation. The main objective to develop this system is, to support? Functional Food Industry? as newly emerging field in agriculture business. To success the system, needs comprehensive applying agriculture biotechnologies, health biotechnologies and also information technologies, in agriculture. By this system, production of valuable bioactive compounds is an advantage, because the market size of functional food is increasing more and more in the future. (Author)

  11. Embedded system-based incubator cultivation of applied talent%基于嵌入式系统应用型人才培养基地的建设

    Institute of Scientific and Technical Information of China (English)

    张乐芳

    2012-01-01

    Based on the opportunities confronted with embedded industrial development,the problems and defects in embedded system teaching among domestic universities are analysed.Practices in guiding principles,educational objectives,curriculum design,training content and instructional models,embedded system-based incubator cultivation of applied talents in Xi'an Eurasia University as an example,are also covered.The author concludes that with embedded system teaching,students' development and application competence has been greatly enhanced and it has also promoted the discipline development and the building of high-quality teaching faculty.%针对嵌入式产业发展所面临的机遇,简要分析了目前国内高校在嵌入式系统教学中存在的问题和不足。基于我院嵌入式系统应用型人才培养基地的建设,介绍了在实施嵌入式人才培养中,从指导思想、培养目标、课程设置、实训内容、教学模式等方面的做法。实践证明,学生在嵌入式开发及应用能力方面有了明显的提升,同时又推动了学科发展及师资队伍建设。

  12. Exploration on Effective Teaching Methods of Ideological and Moral Cultivation Course in Junior High Schools%初中思想品德课有效教学法探究

    Institute of Scientific and Technical Information of China (English)

    秦树芬

    2014-01-01

    初中学生正处于青春期,思想品德课对于学生的德育的养成有着重要作用。提高初中思想品德课的教学有效性就要做到:立足教材,营造师生双方互动的课堂。以学生为中心,营造学生喜欢的课堂。以生活为模板,营造师生共乐的课堂。%Junior high school students are adolescents, so ideo-logical and moral cultivation course plays an important role in the development of their moral cultivation. To improve the effective-ness of ideological and moral cultivation teaching in junior high schools, a classroom with teacher-student interaction should be established based on the textbook;a classroom loved by students should be constructed centered by students;a classroom in which both the teacher and students are happy with life as the back-ground.

  13. Topology based methods for vector field comparisons

    Science.gov (United States)

    Batra, Rajesh Kumar

    Vector fields are commonly found in almost all branches of the physical sciences. Aerodynamics, dynamical systems, electromagnetism, and global climate modeling are a few examples. These multivariate data fields are often large, and no general, automated method exists for comparing these fields. Existing methods require either subjective visual judgments, or data interface compatibility, or domain specific knowledge. A topology based method intrinsically eliminates all of the above limitations and has the additional advantage of significantly compressing the vector field by representing only key features of the flow. Therefore, large databases are compactly represented and quickly searched. Topology is a natural framework for the study of many vector fields. It provides rules of an organizing principle, a flow grammar, that can describe and connect together the properties common to flows. Helman and Hesselink first introduced automated methods to extract and visualize this grammar. This work extends their method by introducing automated methods for vector topology comparison. Basic two-dimensional flows are first compared. The theory is extended to compare three-dimensional flow fields and the topology on no-slip surfaces. Concepts from graph theory and linear programming are utilized to solve these problems. Finally, the first automated method for higher order singularity comparisons is introduced using mathematical theories from geometric (Clifford) algebra.

  14. Facial Beautification Method Based on Age Evolution

    Institute of Scientific and Technical Information of China (English)

    CHEN Yan; DING Shou-hong; HU Gan-le; MA Li-zhuang

    2013-01-01

    This paper proposes a new facial beautification method using facial rejuvenation based on the age evolution. Traditional facial beautification methods only focus on the color of skin and deformation and do the transformation based on an experimental standard of beauty. Our method achieves the beauty effect by making facial image looks younger, which is different from traditional methods and is more reasonable than them. Firstly, we decompose the image into different layers and get a detail layer. Secondly, we get an age-related parameter:the standard deviation of the Gaussian distribution that the detail layer follows, and the support vector machine (SVM) regression is used to fit a function about the age and the standard deviation. Thirdly, we use this function to estimate the age of input image and generate a new detail layer with a new standard deviation which is calculated by decreasing the age. Lastly, we combine the original layers and the new detail layer to get a new face image. Experimental results show that this algo-rithm can make facial image become more beautiful by facial rejuvenation. The proposed method opens up a new way about facial beautification, and there are great potentials for applications.

  15. Identification of suitable sites for mountain ginseng cultivation using GIS and geo-temperature.

    Science.gov (United States)

    Kang, Hag Mo; Choi, Soo Im; Kim, Hyun

    2016-01-01

    This study was conducted to explore an accurate site identification technique using a geographic information system (GIS) and geo-temperature (gT) for locating suitable sites for growing cultivated mountain ginseng (CMG; Panax ginseng), which is highly sensitive to the environmental conditions in which it grows. The study site was Jinan-gun, South Korea. The spatial resolution for geographic data was set at 10 m × 10 m, and the temperatures for various climatic factors influencing CMG growth were calculated by averaging the 3-year temperatures obtained from the automatic weather stations of the Korea Meteorological Administration. Identification of suitable sites for CMG cultivation was undertaken using both a conventional method and a new method, in which the gT was added as one of the most important factors for crop cultivation. The results yielded by the 2 methods were then compared. When the gT was added as an additional factor (new method), the proportion of suitable sites identified decreased by 0.4 % compared with the conventional method. However, the proportion matching real CMG cultivation sites increased by 3.5 %. Moreover, only 68.2 % corresponded with suitable sites identified using the conventional factors; i.e., 31.8 % were newly detected suitable sites. The accuracy of GIS-based identification of suitable CMG cultivation sites improved by applying the temperature factor (i.e., gT) in addition to the conventionally used factors.

  16. Project Based Learning and Cultivation of Students' Professional Abilities%项目教学法与学生职业能力培养

    Institute of Scientific and Technical Information of China (English)

    杨建平

    2012-01-01

    Project Based Learning in professional requirements as a starting point,based on the cultivation of students' professional ability as the core,can achieve the unification of education and jobs,the organic combination of teaching and practical work. Project Based Learning has important significance to strengthen practice teaching to enhance students' professional ability. The reasonable design project tasks,elaborate organization project implementation plan,pay attention to communication and evaluation is the development of Project Based Learning in the process of several important link. Project Based Learning to realize the requirement for the teacher role conversion,and strive to improve their practical ability.%项目教学法以职业岗位需求为出发点,以培养学生的职业能力为核心,可以实现专业教育与职业岗位、教学与实际工作的有机结合。项目教学法对强化实践教学、提升学生的职业能力具有重要意义。合理设计项目任务、精心组织实施项目计划、重视交流和评价是开展项目教学法过程中的几个重要环节。项日教学法要求老师实现角色转换,努力提升自身的实践能力。

  17. 论高校辅导员法治思维的养成%On cultivating college instuctor's law -based thinking

    Institute of Scientific and Technical Information of China (English)

    董延彪

    2015-01-01

    党的十八大以来,中共中央多次强调要全面推进依法治国战略,要运用法治思维和法治方式深化改革,推动发展,化解矛盾,维护稳定。具备法治思维是辅导员依法开展学生工作的前提,是推进学生工作法治化、化解学生工作矛盾的有力武器。本文从辅导员专业化和职业化发展、管理观念、管理规范三个方面探讨了高校辅导员队伍具备法治思维的现实意义,并从尚法,学法,用法三个方面论述了高校辅导员法治思维养成的路径。%Since the 18th CPC national congress, the central committee of CPC has emphasized many times that we should comprehensively advance the rule of law and use the law -based thinking and approaches to deepen the reform , promote de-velopment, resolve conflicts, and maintain stability.In college the quality of law -based thinking is the precondition of col -lege student administration carried out under the rule of law , and also a powerful weapon of promoting law -based administra-tion and resolving college students affairs contradictions .The research analysed the practical significance of college instuctor 'quality of law -based thinking from three aspects and sated how to cultivate college instuctor 'quality of law -based thinking from three aspects, that is, believing law, learning law and use law in student administration .

  18. An image mosaic method based on corner

    Science.gov (United States)

    Jiang, Zetao; Nie, Heting

    2015-08-01

    In view of the shortcomings of the traditional image mosaic, this paper describes a new algorithm for image mosaic based on the Harris corner. Firstly, Harris operator combining the constructed low-pass smoothing filter based on splines function and circular window search is applied to detect the image corner, which allows us to have better localisation performance and effectively avoid the phenomenon of cluster. Secondly, the correlation feature registration is used to find registration pair, remove the false registration using random sampling consensus. Finally use the method of weighted trigonometric combined with interpolation function for image fusion. The experiments show that this method can effectively remove the splicing ghosting and improve the accuracy of image mosaic.

  19. Reinforcement Learning Based on Active Learning Method

    CERN Document Server

    Sagha, Hesam; Khasteh, Hosein; Kiaei, Ali Akbar

    2010-01-01

    In this paper, a new reinforcement learning approach is proposed which is based on a powerful concept named Active Learning Method (ALM) in modeling. ALM expresses any multi-input-single-output system as a fuzzy combination of some single-input-singleoutput systems. The proposed method is an actor-critic system similar to Generalized Approximate Reasoning based Intelligent Control (GARIC) structure to adapt the ALM by delayed reinforcement signals. Our system uses Temporal Difference (TD) learning to model the behavior of useful actions of a control system. The goodness of an action is modeled on Reward- Penalty-Plane. IDS planes will be updated according to this plane. It is shown that the system can learn with a predefined fuzzy system or without it (through random actions).

  20. AN SVAD ALGORITHM BASED ON FNNKD METHOD

    Institute of Scientific and Technical Information of China (English)

    Chen Dong; Zhang Yan; Kuang Jingming

    2002-01-01

    The capacity of mobile communication system is improved by using Voice Activity Detection (VAD) technology. In this letter, a novel VAD algorithm, SVAD algorithm based on Fuzzy Neural Network Knowledge Discovery (FNNKD) method is proposed. The performance of SVAD algorithm is discussed and compared with traditional algorithm recommended by ITU G.729B in different situations. The simulation results show that the SVAD algorithm performs better.

  1. Optimization of Milk-Based Medium for Efficient Cultivation of Bifidobacterium pseudocatenulatum G4 Using Face-Centered Central Composite-Response Surface Methodology

    OpenAIRE

    2014-01-01

    This study was undertaken to optimize skim milk and yeast extract concentration as a cultivation medium for optimal Bifidobacteria pseudocatenulatum G4 (G4) biomass and β -galactosidase production as well as lactose and free amino nitrogen (FAN) balance after cultivation period. Optimization process in this study involved four steps: screening for significant factors using 23 full factorial design, steepest ascent, optimization using FCCD-RSM, and verification. From screening steps, skim milk...

  2. 培育人才创新动力:知识型服务业发展的关键%Cultivating Talents as the Power of Innovation:the Key to the Development of Knowledge-based Service Industry

    Institute of Scientific and Technical Information of China (English)

    王凌

    2013-01-01

    Accelerating the development of knowledge-based service industry ought to be the strategic choice of China’s economic restructuring and development. The cultivation of talent innovation impetus determines the development pace of knowledge-based service industry for a period of time in future. The impetuses for talents in mental and material basis, career development,cultural values and external coordination are the major factors that influence the effect of cultivation.Playing the“enhancing,regulating and guiding”role during such cultivation will be the specific duties of the government as an important cultivation subject.%加快发展知识型服务业是中国经济转型发展的战略选择,行业人才创新动力的培育决定着今后一段时期知识型服务业的加快发展程度。人才心智动力、物质基础动力、职业发展动力、文化价值动力、组织外部动力是影响培育成效的重要因素,发挥好政府在培育中的“充实、规范、引导”作用则是政府作为培育重要主体的具体职责实现。

  3. Cultivating Common Ground: Integrating standards-based visual arts, math and literacy in high-poverty urban classrooms

    OpenAIRE

    Cunnington, Marisol; Kantrowitz, Andrea; Harnett, Susanne; Hill-Ries, Aline

    2014-01-01

    The Framing Student Success: Connecting Rigorous Visual Arts, Math and Literacy Learning experimental demonstration project was designed to develop and test an instructional program integrating high-quality, standards-based instruction in the visual arts, math, and literacy. Developed and implemented by arts-in-education organization Studio in a School (STUDIO), in partnership with the New York City Department of Education, the Framing Student Success curriculum was designed by e...

  4. 基于跨文化交际能力培养的大学英语教学模式探究*%Exploration on College English Teaching Model Based on Cultivating Cross-cultural Communication Ability

    Institute of Scientific and Technical Information of China (English)

    糜艳庆

    2013-01-01

    With economic globalization promoting cultural globalization,the cultural diversity of the world strongly impacts the talents cultivation mode of higher education in China.Internationalization should be-come an important direction of the Chinese higher education development in the future.College English teaching should not only emphasize the cultivation of the students’basic language skills,but should focus on an international and cross-cultural concept to cultivate internationalized talents with international vision and thorough understanding of international rules.The core issues of college English teaching model based on cultivating cross-cultural communication ability are teaching concepts,curriculum and teaching methods reform.The concept of multiculturalism and the two-way cross-cultural communication should be estab-lished and cultural hegemony and the loss of Chinese culture should be alerted as well.The curriculum sys-tem should be established including the core curriculum (college English),English curriculum for special purposes (ESP)and elective curriculum (culture and communication).The classroom teaching mode of Chinese teachers in collaboration with foreign teachers teaching should be enforced.%经济全球化带动文化全球化,世界文化的多样性强烈冲击着我国高等教育的人才培养模式。“国际化”应该成为今后我国高等教育发展的一个重要方向。对于大学英语来说,其教学不能只关注学生基础语言技能的培养,而要“以面向国际和跨文化的理念为中心”,为培养具有国际视野和通晓国际规则的国际化人才服务;基于跨文化交际能力培养的大学英语教学模式的核心问题是教学理念、课程设置和教学方法的改革;树立多元文化及双向跨文化交际的理念,警惕文化霸权主义及中国文化缺失;建立由核心课程(大学英语)、专业课程(ESP)和选修课程(文化与交际)构成的课

  5. Transition in nori cultivation

    DEFF Research Database (Denmark)

    Delaney, Alyne

    2011-01-01

    undertaken in Northeastern Japan among a community of nori cultivators on their production process and cultural way of life. Natural scientists acknowledge that in order to manage natural resources, it is actually the resource users who must be managed. In order to manage resource users, with the goals...... of social and environmental sustainability, we must understand both society and cultural institutions. With this in mind, this article focuses on the division of labor among cultivators, particularly along gender lines and the impacts, on a cultural level, of technological change on nori production...

  6. iTRAQ-based quantitative proteomic analysis reveals proteomic changes in leaves of cultivated tobacco (Nicotiana tabacum) in response to drought stress.

    Science.gov (United States)

    Xie, He; Yang, Da-Hai; Yao, Heng; Bai, Ge; Zhang, Yi-Han; Xiao, Bing-Guang

    2016-01-15

    Drought is one of the most severe forms of abiotic stresses that threaten the survival of plants, including crops. In turn, plants dramatically change their physiology to increase drought tolerance, including reconfiguration of proteomes. Here, we studied drought-induced proteomic changes in leaves of cultivated tobacco (Nicotiana tabacum), a solanaceous plant, using the isobaric tags for relative and absolute quantitation (iTRAQ)-based protein labeling technology. Of identified 5570 proteins totally, drought treatment increased and decreased abundance of 260 and 206 proteins, respectively, compared with control condition. Most of these differentially regulated proteins are involved in photosynthesis, metabolism, and stress and defense. Although abscisic acid (ABA) levels greatly increased in drought-treated tobacco leaves, abundance of detected ABA biosynthetic enzymes showed no obvious changes. In contrast, heat shock proteins (HSPs), thioredoxins, ascorbate-, glutathione-, and hydrogen peroxide (H2O2)-related proteins were up- or down-regulated in drought-treated tobacco leaves, suggesting that chaperones and redox signaling are important for tobacco tolerance to drought, and it is likely that redox-induced posttranslational modifications play an important role in modulating protein activity. This study not only provides a comprehensive dataset on overall protein changes in drought-treated tobacco leaves, but also shed light on the mechanism by which solanaceous plants adapt to drought stress.

  7. Need to reform education in graduate schools of public health in Japan: Toward outcome-based education to cultivate competencies.

    Science.gov (United States)

    Inoue, Mariko; Yano, Eiji

    2016-01-01

    Japan, known for its good healthcare access via universal health insurance, leads the world in terms of life expectancy, and possesses a public health system that has improved health standards markedly in the 20th century. However, we currently face major challenges to maintain and promote people's health. Although these complicated problems pose numerous threats to public welfare, education of public health for health professionals still retains 20th-century standards. This also means that graduate education of public health in Japan is traditionally based on obtaining licensure as a medical professional, conducting research and writing papers, and on-the-job training. Since graduate school education is expected to produce competent public health leaders, Japan requires a reform toward a new education design that caters to the current societal needs. The current global trend in the education of health professionals leans toward outcome-based education to meet core competencies. Here, "competency" refers to a set of features or particular behavioral patterns possessed by highly qualified persons. In 2006, the World Health Organization (WHO) established a general health professional competency standard that includes both management and leadership competencies. Moreover, the Lancet Commission concluded that there was a need for transformative education based on a "health system approach." In brief, this means that our education should correspond to the needs of the health system to allow for the resolution of problems by educated professionals with satisfactory levels of competencies. In addition, as "change agents," these competent professionals are expected to promote societal change toward the realization of better public health. In Japan, the Central Education Council has produced several reports on professional graduate school reform since 2000. These reports indicate that graduate school curricula require reform to allow the health professionals to work

  8. Need to reform education in graduate schools of public health in Japan: Toward outcome-based education to cultivate competencies.

    Science.gov (United States)

    Inoue, Mariko; Yano, Eiji

    2016-01-01

    Japan, known for its good healthcare access via universal health insurance, leads the world in terms of life expectancy, and possesses a public health system that has improved health standards markedly in the 20th century. However, we currently face major challenges to maintain and promote people's health. Although these complicated problems pose numerous threats to public welfare, education of public health for health professionals still retains 20th-century standards. This also means that graduate education of public health in Japan is traditionally based on obtaining licensure as a medical professional, conducting research and writing papers, and on-the-job training. Since graduate school education is expected to produce competent public health leaders, Japan requires a reform toward a new education design that caters to the current societal needs. The current global trend in the education of health professionals leans toward outcome-based education to meet core competencies. Here, "competency" refers to a set of features or particular behavioral patterns possessed by highly qualified persons. In 2006, the World Health Organization (WHO) established a general health professional competency standard that includes both management and leadership competencies. Moreover, the Lancet Commission concluded that there was a need for transformative education based on a "health system approach." In brief, this means that our education should correspond to the needs of the health system to allow for the resolution of problems by educated professionals with satisfactory levels of competencies. In addition, as "change agents," these competent professionals are expected to promote societal change toward the realization of better public health. In Japan, the Central Education Council has produced several reports on professional graduate school reform since 2000. These reports indicate that graduate school curricula require reform to allow the health professionals to work

  9. Research on the method for removing the influential factor for lymphocyte growth during cultivation%去除影响淋巴细胞培养生长因素的方法探讨

    Institute of Scientific and Technical Information of China (English)

    聂俊玮; 梁燕; 杨寿; 唐玉芬

    2008-01-01

    目的 探讨影响淋巴细胞培养生长因素及去除方法.方法 在正常淋巴细胞培养中去掉原培养液,加入灭菌生理水(内含有青霉素钠100 IU/mL)洗涤后再加入培养基继续培养,常规收获细胞及常规G显带油镜观察分析.结果 用上述方法洗涤后收获的细胞较正常培养收获的细胞转化率、有丝分裂指数、分裂相质量均提高非常显著(P<0.01).结论 该方法可去除影响淋巴细胞培养生长的因素,保证收获细胞数量和质量,从而提高分析诊断水平,且易于推广应用.%Objective To probe into the influential factor for lymphocyte growth and its removing method.Methods The original culture liquid in normal lymphocyte medium was removed and washed with the disinfectant physiological saline(containing 100 IU/mL of benzylpenicillin sodium).After that,the new culture medium was added for continuous cultivation.The lymphocytes had been collected conventionally and G banding analysis under immersion objective was performed.Results After the aforesaid washing method was used,cell transformation efficiency,mitotic index and quality of mitotic phase of lymphocytes were obviously improved compared with those cultivated by conventional cultivation method(P<0.01).Conclusion It is proved that this new method can remove the influence on lymphocyte growth during cultivation and guarantee the harvesting cell number and quality,and then improve the levels of analysis and diagnosis.And also it is easy for popularization.

  10. Lagrangian based methods for coherent structure detection

    Energy Technology Data Exchange (ETDEWEB)

    Allshouse, Michael R., E-mail: mallshouse@chaos.utexas.edu [Center for Nonlinear Dynamics and Department of Physics, University of Texas at Austin, Austin, Texas 78712 (United States); Peacock, Thomas, E-mail: tomp@mit.edu [Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 (United States)

    2015-09-15

    There has been a proliferation in the development of Lagrangian analytical methods for detecting coherent structures in fluid flow transport, yielding a variety of qualitatively different approaches. We present a review of four approaches and demonstrate the utility of these methods via their application to the same sample analytic model, the canonical double-gyre flow, highlighting the pros and cons of each approach. Two of the methods, the geometric and probabilistic approaches, are well established and require velocity field data over the time interval of interest to identify particularly important material lines and surfaces, and influential regions, respectively. The other two approaches, implementing tools from cluster and braid theory, seek coherent structures based on limited trajectory data, attempting to partition the flow transport into distinct regions. All four of these approaches share the common trait that they are objective methods, meaning that their results do not depend on the frame of reference used. For each method, we also present a number of example applications ranging from blood flow and chemical reactions to ocean and atmospheric flows.

  11. 虚拟情境教学法在专业英语人才培养中的应用研究%Virtual Situational Teaching Method in the Application of Professional English Talent Cultivation Research

    Institute of Scientific and Technical Information of China (English)

    王梅亮; 叶秋群

    2014-01-01

    为了适应新的经济时代对应用型英语专业人才的需求,迫切需要改革目前专业英语的教学模式。该文首先根据建构主义教学理论,探讨虚拟情境教学法在专业英语教学中应用的优越性;然后研究如何在专业英语课堂教学中利用虚拟情境教学系统,为学生营造出逼真的语言学习和运用环境,通过情景体验进行教学和操练来提高学英语的学习效果,为培养应用型英语专业人才探索出一种全新而且有效的教学模式和教学手段。%In order to adapt to the new economic era demand for applied English professional talents, the current professional English teaching mode need to be reformed urgently.The paper based on the constructivism teaching theory, discusses the applica-tion of virtual situational teaching method in the professional English teaching superiority. And then it studied how to use virtual situation teaching in English classroom teaching system, in order to create a vivid language learning and using environment for students. Through the scene experience and practice of teaching to improve the learning effect of learning English, we can culti-vate applied English professional talents and explore a new effective teaching mode and teaching means.

  12. Life cycle assessment of hemp cultivation and use of hemp-based thermal insulator materials in buildings.

    Science.gov (United States)

    Zampori, Luca; Dotelli, Giovanni; Vernelli, Valeria

    2013-07-01

    The aim of this research is to assess the sustainability of a natural fiber, such as hemp (Cannabis sativa), and its use as thermal insulator for building applications. The sustainability of hemp was quantified by life cycle assessment (LCA) and particular attention was given to the amount of CO2eq of the whole process, and the indicator greenhouse gas protocol (GGP) was selected to quantify CO2eq emissions. In this study also CO2 uptake of hemp was considered. Two different allocation procedures (i.e., mass and economic) were adopted. Other indicators, such as Cumulative Energy Demand (CED) and EcoIndicator99 H were calculated. The production of 1 ha yielded 15 ton of hemp, whose global warming potential (GWP100) was equal to about -26.01 ton CO2eq: the amount allocated to the technical fiber (20% of the total amount of hemp biomass) was -5.52 ton CO2eq when mass allocation was used, and -5.54 ton CO2eq when economic allocation was applied. The sustainability for building applications was quantified by considering an insulation panel made by hemp fiber (85%) and polyester fiber (15%) in 1 m(2) of wall having a thermal transmittance (U) equal to 0.2 W/m(2)_K. The environmental performances of the hemp-based panel were compared to those of a rockwool-based one.

  13. Phase space bases for response matrix methods

    International Nuclear Information System (INIS)

    New basis functions in space, angle, and energy are developed for use in the eigenvalue response matrix method (ERMM). ERMM solves reactor eigenvalue problems by spatially decomposing a model and linking the resulting independent nodes through approximate boundary conditions. The conditions are defined via a set of nodal transport calculations for which the forcing function is a tensor product of basis functions in all phase space variables on one nodal surface (with vacuum elsewhere). Traditional implementations of ERMM have used the standard bases of mathematical physics, including Legendre polynomials and their discrete analogs. Alternatively, new bases that incorporate some physics a priori are shown to yield satisfactory results with far lower expansion orders than is possible with more traditional bases. In particular, a very simple spatial basis incorporating pin-dependent spatial variation reduces partial current errors by nearly an order of magnitude over a standard basis. In angle, conservative bases with appropriate angular quadratures outperform simpler bases. In energy, incorporating representative spectral information from infinite medium calculations results in a basis that can achieve sub-1% pin fission rate errors with as low as a 5th order expansion in energy for a 44-group problem. The results indicate that highly-accurate, low-order response matrix solutions should be feasible for reactor physics analysis. (author)

  14. 创新高校学生干部队伍培养之路%Innovation of the Cultivation Methods for College Student Cadre Group

    Institute of Scientific and Technical Information of China (English)

    方君亮; 宣玉梅; 成晓

    2011-01-01

    高校学生干部是大学校园中的一个特殊群体,也是高校学生工作的一个重要方面。如何打造一支高素质的学生干部队伍,对于做好高校的思想政治工作和学生工作都具有重要意义。本文着重研究如何培养学生干部自身素质、如何培养学生干部团队合作意识和如何在活动中提升学生干部队伍综合素质。%Student cadre is a special group on college campus and an important aspect of college student affairs.How to construct a high quality student cadre group is of great significance to implement college ideological and political work and student affairs.This paper mainly studies on how to cultivate the quality of student cadres,how to cultivate the teamwork awareness of student cadre group and how to improve the comprehensive quality of student cadre group.

  15. Cost effective and economic method for cultivation of Chlorella pyrenoidosa for the simultaneous treatment of biogas digester wastewater and biogas production

    Directory of Open Access Journals (Sweden)

    Rohit Sharma

    2015-02-01

    Full Text Available Microalgae have recently received a lot of attention as a new biomass source for the production of bio fuels and for the treatment of waste water. In this work, Chlorella pyrenoidosa was cultivated in biogas digester wastewater. The growth kinetics of the algae as well as the bio-remediation effect on the waste water was studied. The Chlorella pyrenoidosa can utilize the nitrogen content present in biogas digester wastewater as a substrate for its growth. The growth of microalgae was found to follow the Monod growth model satisfactorily. Under the optimal condition in biogas waste water medium of microalgae, a maximum biomass of 1.5 gm/l was obtained in fifteen days. The net specific growth rate of microalgae Chlorella pyrenoidosa was found to be 0.1 D −1. The growing algae also removed 76 % of nitrate nitrogen (NO3-N from the biogas wastewater. Treated Biogas waste water can be further used for the anaerobic digestion of algal biomass for the production of biogas. This suggests that the cultivation of Chlorella pyrenoidosa in biogas wastewater would be efficient, saving water as well as producing digestible biomass. Thus, on one hand the biogas waste water is being treated and on the other, the alga is showing substantial growth. The same algal species can then be used to produce biogas or other corresponding bio fuel.

  16. Chapter 11. Community analysis-based methods

    Energy Technology Data Exchange (ETDEWEB)

    Cao, Y.; Wu, C.H.; Andersen, G.L.; Holden, P.A.

    2010-05-01

    Microbial communities are each a composite of populations whose presence and relative abundance in water or other environmental samples are a direct manifestation of environmental conditions, including the introduction of microbe-rich fecal material and factors promoting persistence of the microbes therein. As shown by culture-independent methods, different animal-host fecal microbial communities appear distinctive, suggesting that their community profiles can be used to differentiate fecal samples and to potentially reveal the presence of host fecal material in environmental waters. Cross-comparisons of microbial communities from different hosts also reveal relative abundances of genetic groups that can be used to distinguish sources. In increasing order of their information richness, several community analysis methods hold promise for MST applications: phospholipid fatty acid (PLFA) analysis, denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (TRFLP), cloning/sequencing, and PhyloChip. Specific case studies involving TRFLP and PhyloChip approaches demonstrate the ability of community-based analyses of contaminated waters to confirm a diagnosis of water quality based on host-specific marker(s). The success of community-based MST for comprehensively confirming fecal sources relies extensively upon using appropriate multivariate statistical approaches. While community-based MST is still under evaluation and development as a primary diagnostic tool, results presented herein demonstrate its promise. Coupled with its inherently comprehensive ability to capture an unprecedented amount of microbiological data that is relevant to water quality, the tools for microbial community analysis are increasingly accessible, and community-based approaches have unparalleled potential for translation into rapid, perhaps real-time, monitoring platforms.

  17. Cultivation of native seaweed Gracilaria domingensis (Rhodophyta in Southern Brazil

    Directory of Open Access Journals (Sweden)

    José Pedrassoli Salles

    2010-06-01

    Full Text Available The aim of this work was to study the cultivation of Gracilaria domingensis in a mussel farming urbanized area in Santa Catarina, Brazil. Relative growth rate was the parameter used to evaluate the cuttings attachment methods on the cultivation rope, cuttings density, cultivation period and cystocarpic versus unfertile thalli performance. The cultivation was feasible only when protected by net cages due to herbivory. The tie-tie attachment method presented the best results. No differences were observed when comparing the cuttings densities and reproductive phase. Future studies should evaluate the cost-effectiveness of producing the species in net cages and its potential as biofilter.

  18. Investigation of the microbial community in the Odisha hot spring cluster based on the cultivation independent approach

    Science.gov (United States)

    Singh, Archana; Subudhi, Enketeswara; Sahoo, Rajesh Kumar; Gaur, Mahendra

    2016-01-01

    Deulajhari hot spring is located in the Angul district of Odisha. The significance of this hot spring is the presence of the hot spring cluster adjacent to the cold spring which attracts the attention of microbiologists to understand the role of physio-chemical factors of these springs on bacterial community structure. Next-generation sequencing technology helps us to depict the pioneering microflora of any ecological niche based on metagenomic approach. Our study represents the first Illumina based metagenomic study of Deulajhari hot spring DH1, and DH2 of the cluster with temperature 65 °C to 55 °C respectively establishing a difference of 10 °C. Comprehensive study of microbiota of these two hot springs was done using the metagenomic sequencing of 16S rRNA of V3‐V4 region extracting metagenomic DNA from the two hot spring sediments. Sequencing community DNA reported about 28 phyla in spring DH1 of which the majority were Chloroflexi (22.98%), Proteobacteria (15.51%), Acidobacteria (14.51%), Chlorobi (9.52%), Nitrospirae (8.54%), and Armatimonadetes (7.07%), at the existing physiochemical conditions like; temperature 65 °C, pH 8.06, electro conductivity 0.020 dSm− 1, and total organic carbon (TOC) 3.76%. About 40 phyla were detected in cluster DH2 at the existing physiochemical parameters like temperature 55 °C, pH 8.10, electro conductivity 0.019 dSm− 1, and total organic carbon (TOC) 0.58% predominated with Chloroflexi (41.98%), Proteobacteria (10.74%), Nitrospirae (10.01%), Chlorobi (8.73%), Acidobacteria (6.73%) and Planctomycetes (3.73%). Approximately 68 class, 107 order, 171 genus and 184 species were reported in cluster DH1 but 102 class, 180 order, 375 genus and 411 species in cluster DH2. The comparative metagenomics study of the Deulajhari hot spring clusters DH1, and DH2 depicts the differential profile of the microbiota. Metagenome sequences of these two hot spring clusters are deposited to the SRA database and are available in

  19. Meshless numerical method based on tensor product

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    A normalized space constructed by tensor product is used in field function approach to give a special case of moving least squares (MLS) interpolation scheme.In the regular domain,the field function which meets homogenous boundary conditions is constructed by spanning base space to make the MLS interpolation scheme simpler and more efficient.Owing to expanded basis functions selection,some drawbacks in general MLS method,for example repeated inversion,low calculation efficiency,and complex criterions,can be avoided completely.Numerical examples illustrate that the proposed method is characterized by simple mathematical concept,convenient repeat calculations with high accuracy,good continuity,less computation and rapid convergence.

  20. Kernel method-based fuzzy clustering algorithm

    Institute of Scientific and Technical Information of China (English)

    Wu Zhongdong; Gao Xinbo; Xie Weixin; Yu Jianping

    2005-01-01

    The fuzzy C-means clustering algorithm(FCM) to the fuzzy kernel C-means clustering algorithm(FKCM) to effectively perform cluster analysis on the diversiform structures are extended, such as non-hyperspherical data, data with noise, data with mixture of heterogeneous cluster prototypes, asymmetric data, etc. Based on the Mercer kernel, FKCM clustering algorithm is derived from FCM algorithm united with kernel method. The results of experiments with the synthetic and real data show that the FKCM clustering algorithm is universality and can effectively unsupervised analyze datasets with variform structures in contrast to FCM algorithm. It is can be imagined that kernel-based clustering algorithm is one of important research direction of fuzzy clustering analysis.

  1. Trinocular Calibration Method Based on Binocular Calibration

    Directory of Open Access Journals (Sweden)

    CAO Dan-Dan

    2012-10-01

    Full Text Available In order to solve the self-occlusion problem in plane-based multi-camera calibration system and expand the measurement range, a tri-camera vision system based on binocular calibration is proposed. The three cameras are grouped into two pairs, while the public camera is taken as the reference to build the global coordinate. By calibration of the measured absolute distance and the true absolute distance, global calibration is realized. The MRE (mean relative error of the global calibration of the two camera pairs in the experiments can be as low as 0.277% and 0.328% respectively. Experiment results show that this method is feasible, simple and effective, and has high precision.

  2. How to Design and Present Texts to Cultivate Balanced Regional Images in Geography Education

    Science.gov (United States)

    Lee, Dong-Min; Ryu, Jaemyong

    2013-01-01

    This article examines possibilities associated with the cultivation of balanced regional images via the use of simple methods. Two experiments based on the primacy effect and the painting picture rule, or visual depiction of regions, were conducted. The results show significant differences in the formation of regional images. More specifically,…

  3. On Task-based English Learning Method

    Institute of Scientific and Technical Information of China (English)

    朱蕾

    2010-01-01

    @@ Task-Based learning(TBL)is becoming a catchword in English circles.The new national English Curricular Syllabus also recommends the use of the TBL approach in classroom teaching.The purpose of learning a foreign language is the most direct communicative in the target language,and speaking is the most direct communicative method.In recent years,with the publication of the New Curriculum Standard by the State Education Department,the teaching reform in middle and primary schools has been being implemented step by step.

  4. Research on Irrigation Methods and Density of Tomato Cultivated in Small Pot%番茄小根域基质栽培:灌溉方式和密度研究

    Institute of Scientific and Technical Information of China (English)

    崔琳茹; 牛庆良; 黄丹枫; 朱宏印; 刘静静

    2012-01-01

    -systems was significant. According to the comparison with tomato planted in potting, as well as fruit yields and quality, cultivating the plants in small pot integrated with filtration irrigation and reasonable close planting based on low density (5. 2 plants/m2) is an advantageous method for production.

  5. 考虑耕地生态质量的基本农田划定方法%Demarcating method of prime farmland considering ecological quality of cultivated land

    Institute of Scientific and Technical Information of China (English)

    任艳敏; 唐秀美; 刘玉; 潘瑜春

    2014-01-01

    Evaluating the quality of cultivated land comprehensively and systematically is an important prerequisite in performing, demarcating, and conducting high standards of prime farmland scientifically. Taking Tunchang county, Hainan Province as an example, these indexes are chosen to evaluate the quality of cultivated land in the paper as following: topography, erosion condition of soil, ecological system service value, flood disaster, connection, etc, on the basis of comprehensive consideration of nature quality and location condition. It shows that the ecological quality is very important in prime farmland evaluation, and index system is improved. The result: 88.91% of cultivated land is demarcated into prime farmland, with comprehensive evaluating score equal or larger than 70, mainly in the central south and north of the platforms and plains regions of Tunchang country. Contrastive analysis was studied between the method of this paper (method 1) and natural quality of agricultural land (method 2) in demarcating prime farmland. Cultivated land of the study area is classified into four type regions, namely: Type region I, Type region II, Type region III, Type region IV. Among them, Type region I is described as the farmland area demarcated by the above two methods, which is the largest part (84.49%) and has some essential characteristics such as good nature quality, convenient location convenient, less natural disasters, and soil erosion, as well as fairly flat land. Except that, prime farmland is close to towns or rural settlements in spatial distribution, and their average patch area is large and is in the obviously centralized distribution, which is easy to agricultural scaled production and mechanization. Type region II is chosen through method 2, only occupies 4.42%of the area, but distributes in every town. This kind of cultivated land is mainly west and east of the mountainous and hilly regions. The patches are much smaller than Type region I or Type region

  6. Evidence for a Common Origin of Blacksmiths and Cultivators in the Ethiopian Ari within the Last 4500 Years: Lessons for Clustering-Based Inference.

    Science.gov (United States)

    van Dorp, Lucy; Balding, David; Myers, Simon; Pagani, Luca; Tyler-Smith, Chris; Bekele, Endashaw; Tarekegn, Ayele; Thomas, Mark G; Bradman, Neil; Hellenthal, Garrett

    2015-08-01

    The Ari peoples of Ethiopia are comprised of different occupational groups that can be distinguished genetically, with Ari Cultivators and the socially marginalised Ari Blacksmiths recently shown to have a similar level of genetic differentiation between them (FST ≈ 0.023 - 0.04) as that observed among multiple ethnic groups sampled throughout Ethiopia. Anthropologists have proposed two competing theories to explain the origins of the Ari Blacksmiths as (i) remnants of a population that inhabited Ethiopia prior to the arrival of agriculturists (e.g. Cultivators), or (ii) relatively recently related to the Cultivators but presently marginalized in the community due to their trade. Two recent studies by different groups analysed genome-wide DNA from samples of Ari Blacksmiths and Cultivators and suggested that genetic patterns between the two groups were more consistent with model (i) and subsequent assimilation of the indigenous peoples into the expanding agriculturalist community. We analysed the same samples using approaches designed to attenuate signals of genetic differentiation that are attributable to allelic drift within a population. By doing so, we provide evidence that the genetic differences between Ari Blacksmiths and Cultivators can be entirely explained by bottleneck effects consistent with hypothesis (ii). This finding serves as both a cautionary tale about interpreting results from unsupervised clustering algorithms, and suggests that social constructions are contributing directly to genetic differentiation over a relatively short time period among previously genetically similar groups. PMID:26291793

  7. Evidence for a Common Origin of Blacksmiths and Cultivators in the Ethiopian Ari within the Last 4500 Years: Lessons for Clustering-Based Inference.

    Directory of Open Access Journals (Sweden)

    Lucy van Dorp

    2015-08-01

    Full Text Available The Ari peoples of Ethiopia are comprised of different occupational groups that can be distinguished genetically, with Ari Cultivators and the socially marginalised Ari Blacksmiths recently shown to have a similar level of genetic differentiation between them (FST ≈ 0.023 - 0.04 as that observed among multiple ethnic groups sampled throughout Ethiopia. Anthropologists have proposed two competing theories to explain the origins of the Ari Blacksmiths as (i remnants of a population that inhabited Ethiopia prior to the arrival of agriculturists (e.g. Cultivators, or (ii relatively recently related to the Cultivators but presently marginalized in the community due to their trade. Two recent studies by different groups analysed genome-wide DNA from samples of Ari Blacksmiths and Cultivators and suggested that genetic patterns between the two groups were more consistent with model (i and subsequent assimilation of the indigenous peoples into the expanding agriculturalist community. We analysed the same samples using approaches designed to attenuate signals of genetic differentiation that are attributable to allelic drift within a population. By doing so, we provide evidence that the genetic differences between Ari Blacksmiths and Cultivators can be entirely explained by bottleneck effects consistent with hypothesis (ii. This finding serves as both a cautionary tale about interpreting results from unsupervised clustering algorithms, and suggests that social constructions are contributing directly to genetic differentiation over a relatively short time period among previously genetically similar groups.

  8. Bus Based Synchronization Method for CHIPPER Based NoC

    Directory of Open Access Journals (Sweden)

    D. Muralidharan

    2016-01-01

    Full Text Available Network on Chip (NoC reduces the communication delay of System on Chip (SoC. The main limitation of NoC is power consumption and area overhead. Bufferless NoC reduces the area complexity and power consumption by eliminating buffers in the traditional routers. The bufferless NoC design should include live lock freeness since they use hot potato routing. This increases the complexity of bufferless NoC design. Among the available propositions to reduce this complexity, CHIPPER based bufferless NoC is considered as one of the best options. Live lock freeness is provided in CHIPPER through golden epoch and golden packet. All routers follow some synchronization method to identify a golden packet. Clock based method is intuitively followed for synchronization in CHIPPER based NoCs. It is shown in this work that the worst-case latency of packets is unbearably high when the above synchronization is followed. To alleviate this problem, broadcast bus NoC (BBus NoC approach is proposed in this work. The proposed method decreases the worst-case latency of packets by increasing the golden epoch rate of CHIPPER.

  9. 基于Unity3D的甘蔗种植虚拟教育培训系统设计与实现%Design and Realization of Virtual Education Training System for Sugarcane Cultivation Based on Unity3D

    Institute of Scientific and Technical Information of China (English)

    吴升; 郭新宇; 贺谊; 温维亮; 王虓

    2014-01-01

    我国是最大的甘蔗种植国之一,甘蔗种植教育培训课程在各大农林职业院校广泛开展,针对传统的甘蔗种植教育培训试验开展周期长、成本高、授课方式枯燥、参与实践机会少等不足。为此开展甘蔗生长模型建模,农事仿真实时可视化等虚拟仿真交互关键技术进行研究;以“看动画、学知识、玩游戏、长技能”的虚拟互动培训理念,提出基于时序驱动的甘蔗虚拟种植教育培训系统设计方法;以Unity3D为开发平台,实现甘蔗种植生长周期虚拟展示以及互动体验功能。系统以虚拟化改造传统教学,为现代农业职业技术教育提供新的教学思路,从而为全面提升学员技术技能水平做出贡献。%China is one of the largest sugarcane cultivation countries. Education training courses for sugarcane cultivation is conducted in vocational colleges of agriculture and forestry universities. On account of the shortcomings of the conventional sugarcane education and training, such as long cycles, high costs, boring teaching methods, few practice opportunities, etc., we carried out studies on key technology of sugarcane interactive virtual simulation, sugarcane growth modeling, sugarcane planting real⁃time visualization simulation, etc. Method for designing sugarcane virtual education training system based on timing driven was provided, taking the concept of “watching animation, learning knowledge, playing games, and mastering skill” throughout the whole teaching process. Thus, taking Unity3D as a development platform, we realized the virtual display of sugarcane growth cycle and interactive experience function. This system took virtualization to reform the traditional education, and provided new teaching ideas for modern vocational and technical education in agriculture, thus had comprehensive upgrading of the students’ technical skill level.

  10. 不同水直播方式水稻植株抗倒特性研究%Lodging Resistance of Rice under the Different Pattern of Water Direct-seeding Cultivation Method

    Institute of Scientific and Technical Information of China (English)

    许轲; 唐磊; 郭保卫; 张洪程; 霍中洋; 戴其根; 魏海燕

    2014-01-01

    cultivation,by meas-uring the morphological and physical traits of rice basal stems. Furthermore, the correlation analysis between the lodging index,breaking resistance and main culm physical characteristics were studied. The results indicated that the culm lodging resistance of rice planted with different planting methods was significantly different. In field,the lodging rate of hole seeding rice was lower than that of seeding rice in drill. Compared with seeding rice in drill,the breaking resistance of the second basal internode and third basal internode in hole seeding rice was higher,while the lodging index was lower. Plant height and gravity center height in hole seeding rice was longer with the ratio of grav-ity center height to plant height shorter. In hole seeding rice,length of the first and second basal internode was sig-nificantly shorter,culm inside diameter of three internodes from base was longer, and beside the first basal inter-node,culm outside diameter and culm wall thickness of the second basal internode and third basal internode were longer,and dry weight of unit internode of the first basal internode and the second basal internode were higher. Those culm physical traits of different type varieties were significantly different. The breaking resistance of the 1st, 2nd,3rd basal internode and plant height,gravity center height,culm diameter,culm wall thickness,dry weight of culm,dry weight of unit internode and bending moment had significant or very significant positive correlation,while internodes length showed a significant or very significant negative correlation. The optimal combination of some main culm physical traits of rice was different under different planting methods. The direct reasons for lodging resistance increased in hole seeding rice were short and thick basal internodes,large wall thickness and good culm plumpness status.

  11. Studies on Edible Fungi Cultivation Based on Oilseed Rape Straw%油菜秸秆栽培食用菌研究综述

    Institute of Scientific and Technical Information of China (English)

    宋海燕; 胡殿明

    2015-01-01

    Edible fungi cultivation is a sufficient way to reuse the massive oilseed rape straw without any environment pollution. In this paper, the literatures reported on edible fungi cultivation using the substrate of oilseed rape straw in China were briefly reviewed mainly on the aspects of medium formulations and fungal species. According to our statistic, the literatures that reported on edible fungi cultivation using oilseed rape straw reported in China were manly focused on the cultivation of Pleurotus spp. and Flammulina velutipes, while only a few literatures reported on the cultivation of Hypsizygus marmoreus, Volvariella volvacea and Lentinus edodes. Most reports just briefly reported the medium formulations for edible fungi cultivation, without other details such as pretreatment of raw materials, records of hyphae growth, temperature, humidity, light, the concentration of CO2, etc. In this paper, the medium formulations using oilseed rape straw reported by literatures were all listed in 3 tables, they included 29 formulations for the cultivation of Pleurotus spp., 13 formulations for the cultivation of Flammulina velutipes and 6 formulations for the cultivation of other edible fungi. The biological transformation efficiency of each formulation using oilseed rape straw was discussed. The biological transformation efficiency of most formulations mainly composed by oilseed rape straw was commonly lower than the biological transformation efficiency of traditional formulations, however, most of the former formulations presented higher economic input­output ratio than the latter, as oilseed rape straw was normally cheaper than the traditional substrates such as cotton seed hulls, sawdust etc. Furthermore, mushroom hyphae normally grew faster on oilseed rape straw substrates than traditional substrates, which could significantly shorten the cultivation period of edible fungi and subsequently reduce the cost of the cultivation of edible fungi. Therefore, oilseed rape

  12. Environment Quality Detection and Assessment for Cultivation Base of Mesona chinensis in Jinxiu County of Guangxi%广西金秀县凉粉草种植基地的环境质量检测与评价

    Institute of Scientific and Technical Information of China (English)

    韦荣昌; 吴庆华; 白隆华; 梁干君; 蓝祖栽; 唐其

    2013-01-01

    To make clear the quality of environmental and product for GAP planting base of M. chinensis, the environmental quality of M. chinensis original producing area in Jinxiu County, Guangxi Province was detected and evaluated using the standard environmental monitoring method issued by Chinese Environmental Protection Bureau. The results showed that the soil quality of M. chinensis cultivation base reached the second class standard of GB15618 - 1995(Environmental Quality Standard for Soil). The irrigation water quality reached the second class standard of GB 5084 - 2005 (Standards for Irrigation Water Quality). And the medicinal materials quality reached WM/T2 - 2004(Green Standards of Medicinal Plants and Preparations for Foreign Trade and Economy). It could be concluded that the environmental quality of the investigated base meets the corresponding requirement of GAP and the base is suitable to plant M. chinensis.%为弄清凉粉草GAP种植基地的环境及成品的质量状况,参照国家环保局环境监测分析方法,对广西金秀县罗香乡山茶村凉粉草种植基地的环境质量进行了检测与评价.结果表明:凉粉草种植基地的土壤质量达GB15618-1995土壤环境质量二级标准,灌溉水质达GB5084-2005农田灌溉水质二类标准,药材质量符合WM/T2-2004药用植物及制剂外经贸绿色行业标准要求.该基地环境质量良好,符合GAP的相关要求,可作为凉粉草规范化种植基地.

  13. Study on a pattern classification method of soil quality based on simplified learning sample dataset

    Science.gov (United States)

    Zhang, Jiahua; Liu, S.; Hu, Y.; Tian, Y.

    2011-01-01

    Based on the massive soil information in current soil quality grade evaluation, this paper constructed an intelligent classification approach of soil quality grade depending on classical sampling techniques and disordered multiclassification Logistic regression model. As a case study to determine the learning sample capacity under certain confidence level and estimation accuracy, and use c-means algorithm to automatically extract the simplified learning sample dataset from the cultivated soil quality grade evaluation database for the study area, Long chuan county in Guangdong province, a disordered Logistic classifier model was then built and the calculation analysis steps of soil quality grade intelligent classification were given. The result indicated that the soil quality grade can be effectively learned and predicted by the extracted simplified dataset through this method, which changed the traditional method for soil quality grade evaluation. ?? 2011 IEEE.

  14. Building a field- and model-based climatology of local water and energy cycles in the cultivated Sahel - annual budgets and seasonality

    Science.gov (United States)

    Velluet, C.; Demarty, J.; Cappelaere, B.; Braud, I.; Issoufou, H. B.-A.; Boulain, N.; Ramier, D.; Mainassara, I.; Charvet, G.; Boucher, M.; Chazarin, J.-P.; Oï, M.; Yahou, H.; Maidaji, B.; Arpin-Pont, F.; Benarrosh, N.; Mahamane, A.; Nazoumou, Y.; Favreau, G.; Seghieri, J.

    2014-05-01

    In the African Sahel, energy and water cycling at the land surface is pivotal for regional climate, water resources and land productivity, yet it is still extremely poorly documented. As a step towards a comprehensive climatological description of surface fluxes in this area, this study provides estimates of average annual budgets and seasonal cycles for two main land use types of the cultivated Sahelian belt, rainfed millet crop and fallow bush. These estimates build on the combination of a 7 year field dataset from two typical plots in southwestern Niger with detailed physically-based soil-plant-atmosphere modelling, yielding a continuous, comprehensive set of water and energy flux and storage variables over the 7 year period. In this study case in particular, blending field data with mechanistic modelling is considered as making best use of available data and knowledge for such purpose. It extends observations by reconstructing missing data and extrapolating to unobserved variables or periods. Furthermore, model constraining with observations compromises between extraction of observational information content and integration of process understanding, hence accounting for data imprecision and departure from physical laws. Climatological averages of all water and energy variables, with associated sampling uncertainty, are derived at annual to subseasonal scales from the 7 year series produced. Similarities and differences in the two ecosystems behaviors are highlighted. Mean annual evapotranspiration is found to represent ~82-85% of rainfall for both systems, but with different soil evaporation/plant transpiration partitioning and different seasonal distribution. The remainder consists entirely of runoff for the fallow, whereas drainage and runoff stand in a 40-60% proportion for the millet field. These results should provide a robust reference for the surface energy- and water-related studies needed in this region. The model developed in this context has the

  15. Building a field- and model-based climatology of local water and energy cycles in the cultivated Sahel – annual budgets and seasonality

    Directory of Open Access Journals (Sweden)

    C. Velluet

    2014-05-01

    Full Text Available In the African Sahel, energy and water cycling at the land surface is pivotal for regional climate, water resources and land productivity, yet it is still extremely poorly documented. As a step towards a comprehensive climatological description of surface fluxes in this area, this study provides estimates of average annual budgets and seasonal cycles for two main land use types of the cultivated Sahelian belt, rainfed millet crop and fallow bush. These estimates build on the combination of a 7 year field dataset from two typical plots in southwestern Niger with detailed physically-based soil-plant-atmosphere modelling, yielding a continuous, comprehensive set of water and energy flux and storage variables over the 7 year period. In this study case in particular, blending field data with mechanistic modelling is considered as making best use of available data and knowledge for such purpose. It extends observations by reconstructing missing data and extrapolating to unobserved variables or periods. Furthermore, model constraining with observations compromises between extraction of observational information content and integration of process understanding, hence accounting for data imprecision and departure from physical laws. Climatological averages of all water and energy variables, with associated sampling uncertainty, are derived at annual to subseasonal scales from the 7 year series produced. Similarities and differences in the two ecosystems behaviors are highlighted. Mean annual evapotranspiration is found to represent ~82–85% of rainfall for both systems, but with different soil evaporation/plant transpiration partitioning and different seasonal distribution. The remainder consists entirely of runoff for the fallow, whereas drainage and runoff stand in a 40–60% proportion for the millet field. These results should provide a robust reference for the surface energy- and water-related studies needed in this region. The model developed in

  16. 当代大学生社会主义道德信仰培育的方式创新%Method innovation in cultivating contemporary college students' socialist moral belief

    Institute of Scientific and Technical Information of China (English)

    陈孔祥

    2016-01-01

    当代大学生社会主义道德信仰培育的方式创新,是新时期我国高校学生德育改革的内在要求。可借鉴我国高校改革开放以来学生社会主义道德信仰培育的经验,在当代大学生社会主义道德信仰培育中实现从“小德育”向“大德育”、从“知性德育”向“生活德育”、从“人际德育”向“生态德育”、从“知识德育”向“能力德育”、从“说教德育”向“实践德育”、从“经验德育”向“创新德育”的转变。%Innovating cultivating method of contemporary college students' socialist moral belief is the inherent requirement of the reform of college students' moral education in China under the new circumstances. We can reference the experience of the cultivation of college students' socialist moral belief since reform and opening up. The cultivation of college students' socialist moral belief needs to achieve transformation from little moral education to great moral education, from intellectually moral education to life moral education, from interpersonal moral education to ecological moral education, from knowledgeable moral education to capable moral education, from preaching moral education to practical moral education, from experienced moral education to innovative moral education.

  17. Application of Reduced Corn Cultivation Technology in Agro-Ecosystem of Cazin Municipality

    Directory of Open Access Journals (Sweden)

    Mirsad Veladžić

    2011-03-01

    Full Text Available Intensive corn cultivation is predominant in current agriculture of the Una-Sana Canton. One of the corn cultivation methods in agro-ecosystem is reduced cultivation. The paper presents the experiment of “Osmak žuti” (eight-row yellow corn cultivation on two control sites with application of reduced and intensive cultivation in Cazin municipality. The objectives of this research were to examine the possibility of application of reduced corn cultivation; analyze statistical variation elements (length, circumference and weight of corn cob; and determine cost effectiveness of reduced relative to intensive production. The results indicate extremely high statistically significant difference (p<0.001 for all parameters in both cultivation methods. The cost effectiveness of reduced relative to intensive cultivation of Osmak žuti corn is higher by 36%. The experiment encourages introduction of reduced corn cultivation practice.

  18. Graph-based Methods for Orbit Classification

    Energy Technology Data Exchange (ETDEWEB)

    Bagherjeiran, A; Kamath, C

    2005-09-29

    An important step in the quest for low-cost fusion power is the ability to perform and analyze experiments in prototype fusion reactors. One of the tasks in the analysis of experimental data is the classification of orbits in Poincare plots. These plots are generated by the particles in a fusion reactor as they move within the toroidal device. In this paper, we describe the use of graph-based methods to extract features from orbits. These features are then used to classify the orbits into several categories. Our results show that existing machine learning algorithms are successful in classifying orbits with few points, a situation which can arise in data from experiments.

  19. Pose measurement method based on geometrical constraints

    Institute of Scientific and Technical Information of China (English)

    Zimiao Zhang; Changku Sun; Pengfei Sun; Peng Wang

    2011-01-01

    @@ The pose estimation method based on geometric constraints is studied.The coordinates of the five feature points in the camera coordinate system are calculated to obtain the pose of an object on the basis of the geometric constraints formed by the connective lines of the feature points and the coordinates of the feature points on the CCD image plane; during the solution process,the scaling and orthography projection model is used to approximate the perspective projection model.%The pose estimation method based on geometric constraints is studied. The coordinates of the five feature points in the camera coordinate system are calculated to obtain the pose of an object on the basis of the geometric constraints formed by the connective lines of the feature points and the coordinates of the feature points on the CCD image plane; during the solution process, the scaling and orthography projection model is used to approximate the perspective projection model. The initial values of the coordinates of the five feature points in the camera coordinate system are obtained to ensure the accuracy and convergence rate of the non-linear algorithm. In accordance with the perspective projection characteristics of the circular feature landmarks, we propose an approach that enables the iterative acquisition of accurate target poses through the correction of the perspective projection coordinates of the circular feature landmark centers. Experimental results show that the translation positioning accuracy reaches ±0.05 mm in the measurement range of 0-40 mm, and the rotation positioning accuracy reaches ±0.06° in the measurement range of 4°-60°.

  20. 基于正态云模型与熵权法的景泰县耕地后备资源开发潜力评价∗%DEVELOPMENTAL POTENTIALITY EVALUATION OF CULTIVATED LAND RESERVE IN JINGTAI BASED ON NORMAL CLOUD AND ENTROPY WEIGHT

    Institute of Scientific and Technical Information of China (English)

    叶达; 吴克宁; 刘霈珈

    2016-01-01

    The development of cultivated land reserve is an important issue related to food security, and the evalu-ation of development potential is the foundation of cultivated land development. Currently, studies on cultivated land reserve in China largely concern with the land suitability evaluation which takes unused land as cultivated land reserve. However, there are few studies discussing the developmental potentiality of cultivated land reserve, which often paid much more attention to natural, social or economic conditions but focused less on ecological condition. Besides, the index weight determined by the traditional studies is always subjective, and the traditional evaluative methods are often incapable of expressing the fuzziness and randomness of the evaluative object precisely. The pur-pose of this study is to establish a feasible evaluating indication system and method for the developmental potentiali-ty of cultivated land reserve based on the statistical data in Jingtai. Methods of entropy weight and the Normal Cloud model are employed. Normal Cloud is a new model using language value to express the uncertain conversion be-tween qualitative description and quantitative description. It can overcome the ambiguity and uncertainty existing in the conversion from qualitative description to quantitative description when using traditional methods to make evalu-ations. Entropy can be used to express the confusion of a system. The weight of evaluation index is greater when the difference between evaluation index is bigger, and it is the basic idea that uses entropy to express the relative im-portance of evaluation index. Methods of entropy weight and Normal Cloud model can effectively reduce the errors caused by the traditional method. The results indicated that the cultivated land reserve developmental potentiality could be evaluated based on three aspects-ecological environment, natural conditions and social economic condi-tions. The evaluation score of each town in

  1. Black truffle cultivation: a global reality

    Directory of Open Access Journals (Sweden)

    Santiago Reyna

    2014-08-01

    Full Text Available Aim of study: In recent decades the cultivation of the black truffle Tuber melanosporum has expanded across all the Mediterranean-climate regions, but also to other regions outside the European standard for the species. We aim to describe the current extent of T. melanosporum cultivation.Area of study: Tuber melanosporum plantations in Europe, the Mediterranean basin, Australia, New Zealand, China, America and South Africa.Material and Methods: The socioeconomic impact of T. melanosporum cultivation, the way in which the current situation has been achieved and the knowledge needed for its progress are reviewed.Research highlights: T. melanosporum has been successfully cultivated in several countries outside its natural area, but many practices are still empirical and thus yields cannot be guaranteed. The recent advances in molecular techniques and genome science are helping to overcome some of the difficulties traditionally constraining truffle research. The role of truffles as a transitional element between agricultural and forestry activities makes its cultivation a paradigm of sustainable rural development.Keywords: Tuber melanosporum; Europe; Australia; New Zealand; Chile; USA.

  2. An Evaluation of the Externality of Cultivated Land Based on CVM:A Case Study of Neihuang County, Henan Province%基于CVM耕地资源利用的外部性评估——以河南省内黄县为例

    Institute of Scientific and Technical Information of China (English)

    陈艳蕊; 赵凯; 王志晓; 薛欧

    2011-01-01

    improve farmland landscape. This helps enhance farmer's awareness to protect cultivated land so as to improve the overall efficiency of land utilization. Based on questionnaire surveys about Neihuang County in Henan Province, we performed Pearson correlation tests about the socio-economic characteristics, and subsequently multiple linear regression analysis on the dependent variables of LnWTP. In addition, we assessed the Externality of the Cultivated Land with Contingent Valuation Method (CVM) and analyzed its influential factors. Results show that: 1) the relationship between annual income of urban households and their willingness to pay is statistically significantly positive; 2) the relationship between the age of rural residents and the corresponding area of contracted cultivated land and their willingness to pay is statistically significantly negative; the relationship among gender, education level, household annual income, agricultural income, and their willingness to pay are generally positive; and 3) based on the median values of 75.5 yuan as the urban residents' willingness to pay, and 15.5 yuan as the willingness to pay of the rural residents, the externality of the cultivated land resources in Neihuang County, Henan Province, was estimated to be 11 649.5 yuan/hm2 (776.6 yuan/mu). Thus, more attention should be paid to the value of the externality of the cultivated land resources in estimating its total value, expropriation price, transaction price, and to policy making with respect to protection of cultivated land resources.

  3. 体育教学中培养学生自信心的方法%Methods of Cultivating students’ Confidence in Physical Education Teaching

    Institute of Scientific and Technical Information of China (English)

    聂鹏

    2014-01-01

    体育作为初中生学习的必备课程之一,有助于丰富学生学习生活,增添学生学习乐趣,提高学生的自信心。新课程改革也提出了体育作为全民运动的活动项目,有利于保持人们的身心健康。而初中生作为祖国未来的接班人,并且正处于身体发育和心理成熟的关键时期,体育运动对培养学生的自信心尤为关键。%The sports as a junior high school one of the essential courses of study,help to enrich the students’ life,increase students’ interest in learning,improve students’ confidence. The new curriculum reform put forward,sports as a national sports event,is conducive to maintaining the physical and mental health of people. And junior high school students as the successors,and are in the physical and psychological maturity of the critical period,sports is especially important in the cultivation of students’ self-confidence ability.

  4. The Exploration and Practice of the Cultivating Models for Competition and Innovation Based Marketing Professionals%基于技能竞赛创新高职营销人才培养模式的探索与实践

    Institute of Scientific and Technical Information of China (English)

    曹成喜

    2012-01-01

    经济的转型急需与区域经济发展相适应的高职营销人才,文章以全国营销大赛为切入口,对创新高职营销人才培养的模式进行实践与探索,提出基于营销大赛创新高职营销专业人才培养模式的八个突破。%The restructuring of the economy entails an urgent need for professional marketing talents who can adapt to regional e- conomic developments. This thesis explores the cultivation models of innovative marketing professionals from the angle of the na- tional marketing competition and proposes several breakthroughs in the cultivating models based on the marketing competition.

  5. 研究性课程中发明创造技法的应用及创新能力培养探讨%Application of Inventive Method and Cultivation of Innovative Ability in Research Programs

    Institute of Scientific and Technical Information of China (English)

    姜长阳; 徐娜; 张冬艳

    2012-01-01

    In order to cultivate the innovative ability of undergraduates, the students who took part in the research programs were encouraged to solved some problems in an innovative way. The problems concerning plant tissue culture and plant cloning which were difficult to deal with were successfully solved, which accelerated the development of research programs. 82 papers were published in less than 5 years, whose first authors were all undergraduates. With the application of inventive method,the innovative consciousness of the students could be awakened,the innovative thinking could be cultivated, the innovative ability could be enhanced.%为培养本科生的创新能力,让参加研究性课程的学生把发明创造技法应用于研学中,解决发现的问题.从而解决了植物组织培养、植物克隆中许多难以解决的问题,促进了研学的顺利进行,仅不到5年的时间,就取得了发表以本科生为第一作者的学术论文82篇等多项成果,并且通过发明创造技法在研学中的应用,也使学生树立创新意识,培养创新思维,提高创新能力.

  6. AOAC method 966.04: preliminary evaluation of cooked meat medium with manganese sulfate for the cultivation of Clostridium sporogenes: precollaborative study.

    Science.gov (United States)

    Tomasino, Stephen F; Samalot-Freire, Luisa C

    2007-01-01

    AOAC Method 966.04, the Sporicidal Activity of Disinfectants Test, is a carrier-based test that provides a qualitative measure of product efficacy against spores of Bacillus subtilis and Clostridium sporogenes. For regulatory purposes, Method 966.04 is accepted by the U.S. Environmental Protection Agency (EPA) and the U.S. Food and Drug Administration (FDA) for the generation of product performance data for sporicides and sterilants. In this study, we report on findings associated with proposed improvements (modifications) to the Clostridium component of the method. Egg meat medium (EMM), the culture medium for C. sporogenes currently specified in the method, is no longer commercially available and finding a suitable replacement is critical. In addition, the use of a nonstandardized extract of raw soil as an amendment to EMM, as stipulated in the current method, may result in a highly variable spore suspension. The primary focus of this study was to find replacements for EMM and soil extract. A carrier count procedure, the establishment of target carrier counts (spores/carrier), and a neutralization confirmation procedure were also evaluated. The study was limited to liquid products tested against Clostridium on a hard surface carrier (porcelain penicylinder). Spore suspensions of C. sporogenes were generated using: (1) EMM with soil extract (EMM/SE), (2) cooked meat medium with soil extract (CMM/SE), and (3) cooked meat medium with 5 microg/mL manganese sulfate (CMM/MnSO4). The titer of the spore suspension, carrier counts, resistance to hydrochloric acid (HCI), and efficacy against 3 liquid sporicidal agents were used to evaluate the potential of CMM and MnSO4 as replacements. The study was performed by the EPA Office of Pesticide Programs Microbiology Laboratory, Fort Meade, MD. Use of CMM/SE and CMM/MnSO4 resulted in comparable results for titer of spore suspensions (approximately 10(8) spores/mL) and carrier counts (approximately 3 x 10(6) spores/carrier). The

  7. Rice Cultivation Methods and Their Sustainability Aspects: Organic and Conventional Rice Production in Industrialized Tropical Monsoon Asia with a Dual Cropping System

    Directory of Open Access Journals (Sweden)

    Hung-Chun Lin

    2016-06-01

    Full Text Available Options to tackle the sustainability challenges faced in the production of rice, including global and local environmental perspectives, need to be discussed. Here, the global warming potential, water consumption and cumulative energy demand were analyzed using a life-cycle assessment to highlight the sustainability aspects of rice production in Taiwan, where a mixed organic and conventional rice production with a dual cropping system is practiced. The results show that the conventional farming method practiced in Houbi district contributes less to global warming and annual water consumption and consumes less energy than the organic method practiced in Luoshan village on a grain weight basis. It is also more lucrative for farmers because of the higher rice yield. Considering the yield ratio based on the data from two districts, the regional characteristics are more responsible for these differences. Giving up dual cropping to avail water to other sectors by fallowing during the second cropping season is preferable from the GHG emission and productivity perspectives. However, because water shortages usually occur in the first cropping season, it is more realistic to fallow during the first cropping season when domestic and other industrial users have the higher priority. The results presented here can serve as the foundation for exploring the possibilities of options, such as new biorefinery technologies and water allocation policies, in relation to influences on GHG emissions and the national self-sufficiency of rice.

  8. Cultivation of EFL Learners’Intercultural Competence

    Institute of Scientific and Technical Information of China (English)

    夏晨

    2012-01-01

      Nowadays, educators from home and abroad believe that learning a foreign language means learning a great deal of the foreign civilization and culture. And the utmost goal of language teaching is to cultivate EFL learners’communicative competence, but not merely the linguistic knowledge. Thus, teaching a foreign language is more than teaching new words, expressions and grammar rules, but should also incorporate the target culture elements into language knowledge. In order to improve EFL learners’English proficiency, teachers must make efforts to cultivate EFL learners’intercultural competence and promote their sensibility and adaptability to the differences between Chinese culture and the Western culture. Only by doing so, can EFL learners communicate with native speakers appropriately and smoothly. This pa-per proposes some effective teaching techniques and methods that can be applied in English classroom to cultivate Chinese EFL learners’in-tercultural competence.

  9. Phenylhydrazines in the cultivated mushroom (Agaricus bisporus)

    DEFF Research Database (Denmark)

    Andersson, H. C.; Gry, Jørn

    2004-01-01

    In 1991, the Nordic Working Group on Food Toxicology and Risk Evaluation (NNT) reviewed the available data on phenylhydrazines naturally occurring in the cultivated mushroom. It was concluded that the mushroom may contain about 500 mg of the hydrazine derivatives per kg fresh weight. The hydrazine...... derivatives as well as extracts of the cultivated mushroom were mutagenic to a variable degree in most of the reported short-term tests. The raw mushroom and several of the hydrazines induced tumours when administered to Swiss mice as reported by American scientists. However, reservations were expressed...... as to the design of the studies. Based on this review, and due to the concern expressed, a Nordic project (coordinated by Jørn Gry, Danish Veterinary and Food Administration) was initiated dealing with toxicological and chemical studies on the cultivated mushroom and its phenylhydrazine derivatives in order...

  10. [Cultivation of pathogenic free-living amoebae].

    Science.gov (United States)

    Peng, Heng; Zhu, Huai-Min

    2009-08-01

    The isolation and culture of pathogenic free-living amoebae are useful in the diagnosis and research. This review focuses on the methods of isolation and cultivation of pathogenic free-living amoebae, including sample treatment, culture conditions, passage culture, pathogen detection, and maintenance.

  11. Region-based multisensor image fusion method

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Image fusion should consider the priori knowledge of the source images to be fused, such as the characteristics of the images and the goal of image fusion, that is to say, the knowledge about the input data and the application plays a crucial role. This paper is concerned on multiresolution (MR) image fusion. Considering the characteristics of the multisensor (SAR and FLIR etc) and the goal of fusion, which is to achieve one image in possession of the contours feature and the target region feature. It seems more meaningful to combine features rather than pixels. A multisensor image fusion scheme based on K-means cluster and steerable pyramid is presented. K-means cluster is used to segment out objects in FLIR images. The steerable pyramid is a multiresolution analysis method, which has a good property to extract contours information at different scales. Comparisons are made with the relevant existing techniques in the literature. The paper concludes with some examples to illustrate the efficiency of the proposed scheme.

  12. Land Fiscalization and Cultivated Land Protection: Causal Relationship Based on Provincial Panel Data Analysis%土地财政与耕地保护——基于省际面板数据的因果关系分析

    Institute of Scientific and Technical Information of China (English)

    杜雪君; 黄忠华

    2009-01-01

    With rapid growth of economy, urbanization and industrialization, China' s cultivated land decreased 793 × 10~4 hm~2 from 1998 to 2008. Recent studies indicate land fiscalization is one of the important reasons for land decrease. The purpose of this study is to explore the relationship between land fiscalization and cultivated land change. Methods of Granger causality test, OLS, fixed effect model, random effect model and GMM model were used, based on all China provinces' panel data of cultivated land area, land fiscalization, GDP, population and urbanization level from 1999 to 2006. The Granger causality test result shows cultivated land and land fiscalization have mutual feedback effects. Empirical analysis reveals that land fiscaliztion is an important reason for cultivated land decrease, 1 percentage increase in land fiscaliztion would cause 0. 01 percentage decrease of cultivated land. Land fiscaliztion has two opposite impacts on cultivated land. On the one hand, land fiscaliztion could induce local governments to expand cultivated land occupation in pursuit of fiscal revenue growth and economic development. On the other hand, benefited from the land fiscaliztion growth, more capital investment in land consolidation could reduce land decrease rate.%论文利用1999-2006年我国31个省(直辖市、自治区,因资料所限,不包括台湾省及香港和澳门地区)的面板数据,采用格兰杰因果关系检验、普通最小二乘法、固定效应模型、随机效应模型和动态面板数据模型广义矩等来分析我国土地财政对耕地数量变化的影响.研究结果表明:①土地财政与耕地数量之间存在相互反馈作用;②土地财政是导致我国耕地数量减少的重要原因,其影响的弹性系数为-0.01;③土地财政对耕地保护具有正反两方面作用:一方面,土地财政刺激地方政府形成城市扩张冲动,导致耕地数量减少;另一方面,土地财政带来土地开发整理增加耕地面

  13. Effect of cultivation measures on economic benefit of Larix olgesis pulp forest

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    According to the cultivating practice of Larix olgensis pulp plantation, IRR (Internal revenue rate) and NPV (Net present value) were taken as two economic indices to study the effect of cultivation measurements on economic benefit of Larix olgensis pulp forest. The results showed that the economic benefit of this type of forest is closely related to rotation and site class. Higher economic benefit could be obtained when the rotation is shorter and site class is higher. The planting density also had an obvious influence on economic benefit. On the base of assuring survival rate and conserving rate, the less the fee used in soil preparation and young growth tending is, the higher the economic benefit is. The influence of determined six cultivation meas-ures on economic benefit in sequence was the rotation-site class-density-management fee level-young growth tending in-tensity- soil preparation methods.

  14. High-effective cultivation of Halobacterium salinarum providing with bacteriorhodopsin production under controlled stress.

    Science.gov (United States)

    Kalenov, Sergei V; Baurina, Marina M; Skladnev, Dmitry A; Kuznetsov, Alexander Ye

    2016-09-10

    Submerged growth of Halobacterium salinarum and therefore synthesis of bacteriorhodopsin (BR) and carotenoids depend greatly on products of both chemical and/or photochemical oxidation of medium components and cellular metabolism which act as inhibitors. Some cultivation variants which allowed eliminating an adverse effect of inhibitors on biomass accumulation and BR synthesis are reviewed. The application of activated charcoal or ion exchange resin as adsorbents at preparing inoculums and the main cultivation stages was shown to allow controlling, namely lowering overstress of the halobacterial cells by metabolites. The halobacterial biomass containing BR up to 1,750mgL(-1) and the minimum amount of carotinoids that would BR greatly facilitate isolation was accumulated up to 45gL(-1) during eight-day cultivation with cell recycling through adsorbent suspension in a fed-batch mode. To control BR biosynthesis the express method of BR quantification based on colour shades of cell suspension was developed. PMID:27449487

  15. Identification of Growth Phases and Influencing Factors in Cultivations with AGE1.HN Cells Using Set-Based Methods

    OpenAIRE

    Borchers, S.; Freund, S; Rath, A.; Streif, S; Reichl, U.; Findeisen, R.

    2013-01-01

    Production of bio-pharmaceuticals in cell culture, such as mammalian cells, is challenging. Mathematical models can provide support to the analysis, optimization, and the operation of production processes. In particular, unstructured models are suited for these purposes, since they can be tailored to particular process conditions. To this end, growth phases and the most relevant factors influencing cell growth and product formation have to be identified. Due to noisy and erroneous experimenta...

  16. Triptycene-based ladder monomers and polymers, methods of making each, and methods of use

    KAUST Repository

    Pinnau, Ingo

    2015-02-05

    Embodiments of the present disclosure provide for a triptycene-based A-B monomer, a method of making a triptycene-based A-B monomer, a triptycene-based ladder polymer, a method of making a triptycene-based ladder polymers, a method of using triptycene-based ladder polymers, a structure incorporating triptycene-based ladder polymers, a method of gas separation, and the like.

  17. Building a field- and model-based climatology of local water and energy cycles in the cultivated Sahel - annual budgets and seasonality

    Science.gov (United States)

    Velluet, C.; Demarty, J.; Cappelaere, B.; Braud, I.; Issoufou, H. B.-A.; Boulain, N.; Ramier, D.; Mainassara, I.; Charvet, G.; Boucher, M.; Chazarin, J.-P.; Oï, M.; Yahou, H.; Maidaji, B.; Arpin-Pont, F.; Benarrosh, N.; Mahamane, A.; Nazoumou, Y.; Favreau, G.; Seghieri, J.

    2014-12-01

    In the sub-Saharan Sahel, energy and water cycling at the land surface is pivotal for the regional climate, water resources and land productivity, yet it is still very poorly documented. As a step towards a comprehensive climatological description of surface fluxes in this area, this study provides estimates of long-term average annual budgets and seasonal cycles for two main land use types of the cultivated Sahelian belt: rainfed millet crop and fallow bush. These estimates build on the combination of a 7-year field data set from two typical plots in southwestern Niger with detailed physically based soil-plant-atmosphere modeling, yielding a continuous, comprehensive set of water and energy flux and storage variables over this multiyear period. In the present case in particular, blending field data with mechanistic modeling makes the best use of available data and knowledge for the construction of the multivariate time series. Rather than using the model only to gap-fill observations into a composite series, model-data integration is generalized homogeneously over time by generating the whole series with the entire data-constrained model simulation. Climatological averages of all water and energy variables, with associated sampling uncertainty, are derived at annual to sub-seasonal scales from the time series produced. Similarities and differences in the two ecosystem behaviors are highlighted. Mean annual evapotranspiration is found to represent ~82-85% of rainfall for both systems, but with different soil evaporation/plant transpiration partitioning and different seasonal distribution. The remainder consists entirely of runoff for the fallow, whereas drainage and runoff stand in a 40-60% proportion for the millet field. These results should provide a robust reference for the surface energy- and water-related studies needed in this region. Their significance and the benefits they gain from the innovative data-model integration approach are thoroughly discussed

  18. Effects of Temperature variations on the Super Fine Powderization of Korean Cultivated Wild Ginseng

    Directory of Open Access Journals (Sweden)

    Jin Ho Kim

    2006-12-01

    Full Text Available Objectives : The aim of this study was to find optimal conditions for producing red ginseng from cultivated wild ginseng using the Turbo Mill. Methods : Characteristics of powdered cultivated wild ginseng based on various temperature settings of the Turbo Mill were observed, and changes in the content was measured by HPLC for various ginsenosides. Results : 1. The diameter of cultivated wild ginseng powder ground by the Turbo Mill was around 10㎛. 2. As the temperature rose, presusre, Specific Mechanical Energy(SME, and density decreased, whileas Water Solubility Index(WSI increased. 3. As the temperature rose, super fine powder showed tendency to turn into dark brown. 4. Measuring content changes by HPLC, there was no detection of ginsenoside Rg3 and ginsenosideRg1, Rb1, and Rh2 concentrations decreased with increase in temperature. Conclusions : Super fine powder of cultivated wild ginseng produced by the Turbo Mill promotes easy absorption of effective ingredients by breaking the cell walls. Using this mechanism to produce red ginseng from cultivated wild ginseng, it yielded less than satisfactory results under the current experiment setup. Furtherresearches are needed to verify more suitable condition for the production of red ginseng.

  19. 大学生创业能力研究及其培养%Study on College Students' Entrepreneurship Ability and Its Cultivation Based on Professional Skills Elements

    Institute of Scientific and Technical Information of China (English)

    刘畅

    2011-01-01

    从"职业能力"的研究角度,确定了大学生创业能力的能力要素,并在分析大学生创业能力培养环境的基础上,阐述了大学生创业能力的培养路径,旨在推动"国家、高校、大学生"三位一体的大学生创业软环境的日趋完善。%This paper starts from the significance of college students' entrepreneurship ability cultivation,analyzing the ability elements of college student's entrepreneurship from the research aspect of vocation ability.It also states the cultivation approach of college students' entrepreneurship ability,aiming to improve the college students' entrepreneurship soft environment of the trinity of country,college and students.

  20. 以化学竞赛为契机 着力培养应用型人才%Cultivating Applied Talents Based on Chemistry Competition

    Institute of Scientific and Technical Information of China (English)

    韦寿莲; 汪洪武; 许秀丽; 刘玲; 严子军; 赵建芬

    2011-01-01

    Owing to a large social demand for applied talents, combining the school orientation, the cultivation target and an awareness of chemistry competition among students, it' s studied on how to optimize the teaching resources, to improve the teaching quality and to promote the cultivation of applied talents through chemistry competitions in local colleges.%基于社会对应用型人才的大量需求,结合肇庆学院的办学定位、培养目标及化学化工学院学生对竞赛的认知,研究地方高校如何通过化学竞赛优化教学资源,提高教学质量,促进应用型人才的培养.

  1. Research on the Cultivation Model of Software Technology Specialty Based on CDIO Engineering Education Philosophy%基于CDIO理念的高职软件技术专业人才培养模式研究

    Institute of Scientific and Technical Information of China (English)

    贾家新

    2016-01-01

    In view of present situation of China′s higher vocational college of software technology talent cultivation it is necessary to learn the international advanced the concept of CDIO Engineering Education.Then,we need to establish our own talent cultivation model.The theory curriculum system teaching system and evaluation system were based on CDIO philosophy.Also,it is important to deepen software technology professional talent cultivation model.Meanwhile,weshould improve the quality of personnel training,and enhance highGquality software and technical talents.%针对我国高等职业院校软件技术专业人才培养的现状,借鉴国际先进的 CDIO 工程教育理念,分别从人才培养模式的构建、理论课程体系的构建、实践教学体系的构建、考核评价体系的构建等四个方面进行基于CDIO 理念软件技术专业人才培养的研究,探索软件技术专业人才培养模式的建设与改革,提高人才培养质量,为国家培养更多高素质技能应用型软件技术人才。

  2. Comparative Study on the Human Driving Force of Cultivated Land and Construction Land Use Change in Hubei Province,China

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Change trend of cultivated land and construction land in Hubei Province is analyzed.The years 1998-2003 are the reduction period of cultivated land;the years 2004-2007 are the increase period of cultivated land;the years 1988-2002 are the slow growth period of construction land;and the years of 2003 and 2007 are the increase period of construction land.Based on related social economic statistical data in 1988-2007,human driving forces on cultivated land and construction land in Hubei Province are analyzed quantitatively and comparatively by using SPSS software and mathematical statistics method.Result shows that driving force factors have negative driving effect on cultivated land,and positive driving effect on construction land.Driving force factors have significant differences of driving effects on cultivated land and construction land.Three principal components affecting the changes of cultivated land and construction land are policy,population and economic development level,and gap between urban and rural living standards.The three principal components have different degrees of sensitivity on the two land use types and the effects direction are reverse.The other factor driving abilities are relatively weak,and these factors have relatively stronger driving force on construction land than that on cultivated land.It is put forward that Hubei Province should implement rational allocation of land resources and scientific and rational use in order to ensure land security and to realize the sustainable development of regional economy and society based on the intensive land use,the planning policy constraints,and the agricultural and industrial production.

  3. Evidence for a Common Origin of Blacksmiths and Cultivators in the Ethiopian Ari within the Last 4500 Years: Lessons for Clustering-Based Inference.

    OpenAIRE

    Lucy van Dorp; David Balding; Simon Myers; Luca Pagani; Chris Tyler-Smith; Endashaw Bekele; Ayele Tarekegn; Thomas, Mark G; Neil Bradman; Garrett Hellenthal

    2015-01-01

    The Ari peoples of Ethiopia are comprised of different occupational groups that can be distinguished genetically, with Ari Cultivators and the socially marginalised Ari Blacksmiths recently shown to have a similar level of genetic differentiation between them (FST ≈ 0.023 - 0.04) as that observed among multiple ethnic groups sampled throughout Ethiopia. Anthropologists have proposed two competing theories to explain the origins of the Ari Blacksmiths as (i) remnants of a population that inhab...

  4. Pilot scale land-based cultivation of Saccharina latissima Linnaeus at southern European climate conditions: Growth and nutrient uptake at high temperatures

    DEFF Research Database (Denmark)

    Azevedo, Isabel C.; Silva Marinho, Goncalo; Silva, Diogo M.;

    2016-01-01

    for this species. This fact may be explained by their origin in populations located near the southern distribution boundary, which may have acquired adaptations that increased tolerance to high temperatures. Cultivation of S. latissima using tumble culture in outdoor tanks at southern latitudes...... appears to be feasible even during high temperature periods. Densities around 8 kg m− 3 were effective in keeping epiphytes development low. This system may be used for seaweed monoculture or as a biofilter component of IMTA systems....

  5. New deghosting method based on generalized triangulation

    Institute of Scientific and Technical Information of China (English)

    Bai Jing; Wang Guohong; Xiu Jianjuan; Wang Xiaobo

    2009-01-01

    A new deghosting method baaed on the generalized triangulation is presented. First, two intersection points corresponding to the emitter position are obtained by utilizing two azimuth angles and two elevation angles from two jammed 3-D radars (or 2-D passive sensors). Then, hypothesis testing baaed deghosting method in the multiple target scenarios is proposed using the two intersection points. In order to analyze the performance of the proposed method, the correct association probability of the true targets and the incorrect association probability of the ghost targets are defined. Finally, the Monte Carlo simulations are given for the proposed method compared with the hinge angle method in the cases of both two and three radars. The simulation results show that the proposed method has better performance than the hinge angle method in three radars case.

  6. Mushroom Cultivation in South Korea

    OpenAIRE

    Mustafa Kemal Soylu; Mingu Kang

    2016-01-01

    Mushroom cultivation in South Korea is increasing fast last decades. Mushroom cultivation of South Korea is 173577 tones and South Korea gains 800 million dollars income annually. Different kind of mushroom species are cultivated and 31% enoki mushroom (Flammulina velutipes), 26% king oyster (Pleurotus eryngii), 26% oyster mushroom (Pleurotus ostreatus), 13% white buton mushroom (Agaricus bisporus) and 4% rest of the total mushroom Lentinula edodes, Ganoderma lucidum, Phellinus vb. are produc...

  7. Surface modification of closed plastic bags for adherent cell cultivation

    Science.gov (United States)

    Lachmann, K.; Dohse, A.; Thomas, M.; Pohl, S.; Meyring, W.; Dittmar, K. E. J.; Lindenmeier, W.; Klages, C.-P.

    2011-07-01

    In modern medicine human mesenchymal stem cells are becoming increasingly important. However, a successful cultivation of this type of cells is only possible under very specific conditions. Of great importance, for instance, are the absence of contaminants such as foreign microbiological organisms, i.e., sterility, and the chemical functionalization of the ground on which the cells are grown. As cultivation of these cells makes high demands, a new procedure for cell cultivation has been developed in which closed plastic bags are used. For adherent cell growth chemical functional groups have to be introduced on the inner surface of the plastic bag. This can be achieved by a new, atmospheric-pressure plasma-based method presented in this paper. The method which was developed jointly by the Fraunhofer IST and the Helmholtz HZI can be implemented in automated equipment as is also shown in this contribution. Plasma process gases used include helium or helium-based gas mixtures (He + N2 + H2) and vapors of suitable film-forming agents or precursors such as APTMS, DACH, and TMOS in helium. The effect of plasma treatment is investigated by FTIR-ATR spectroscopy as well as surface tension determination based on contact angle measurements and XPS. Plasma treatment in nominally pure helium increases the surface tension of the polymer foil due to the presence of oxygen traces in the gas and oxygen diffusing through the gas-permeable foil, respectively, reacting with surface radical centers formed during contact with the discharge. Primary amino groups are obtained on the inner surface by treatment in mixtures with nitrogen and hydrogen albeit their amount is comparably small due to diffusion of oxygen through the gas-permeable bag, interfering with the plasma-amination process. Surface modifications introducing amino groups on the inner surface turned out to be most efficient in the promotion of cell growth.

  8. A Novel Method For Speech Segmentation Based On Speakers' Characteristics

    CERN Document Server

    Abdolali, Behrouz; 10.5121/sipij.2012.3205

    2012-01-01

    Speech Segmentation is the process change point detection for partitioning an input audio stream into regions each of which corresponds to only one audio source or one speaker. One application of this system is in Speaker Diarization systems. There are several methods for speaker segmentation; however, most of the Speaker Diarization Systems use BIC-based Segmentation methods. The main goal of this paper is to propose a new method for speaker segmentation with higher speed than the current methods - e.g. BIC - and acceptable accuracy. Our proposed method is based on the pitch frequency of the speech. The accuracy of this method is similar to the accuracy of common speaker segmentation methods. However, its computation cost is much less than theirs. We show that our method is about 2.4 times faster than the BIC-based method, while the average accuracy of pitch-based method is slightly higher than that of the BIC-based method.

  9. Study on Economic growth, Urbanization and Sustainable Utilization of Cultivated Land in China

    Institute of Scientific and Technical Information of China (English)

    Wu Qun; Guo Guancheng; Fan Li

    2004-01-01

    By means of the dynamic regression model, this paper analyzes the relationships among economic growth, urbanization and changes of cultivated land in China, finds that the ratio of cultivated land occupied by economic growth is decreasing with social and economic growth. And,based on that, some policy suggestions on how to promote the sustainable use of cultivated land in China are put forward.

  10. Research on Monitoring Area Division of Quality Grade Changes in County Cultivated Land and Technology of Deploying Monitoring Point

    OpenAIRE

    Wei, Wei; Liao, Lijun; Yu, Jianxin

    2013-01-01

    It is an important means in management of improving both the quality and quantity of cultivated land to monitor grade changes in cultivated land quality. How to deploy monitoring network system and its point reasonably and roundly are the key to the technology of monitoring grade changes in cultivated land quality by monitoring grade changes in cultivated land quality dynamically in order to obtain the information to the index of cultivated land quality and its changes based on the existing a...

  11. CONTENT AND UPTAKE OF SELECTED TRACE ELEMENTS BY WEEDS IN POTATO TO CULTIVATION UNDER DIFFERENT CONDITIONS OF SOIL TILLAGE AND WEED CONTROL METHODS

    OpenAIRE

    Krystyna Zarzecka; Marek Gugała; Alicja Baranowska

    2014-01-01

    The study utilized data from a field experiment carried out at the Experimental Station in Zawady owned by the University of Natural Sciences and Humanities in Siedlce in the years 2005–2007. The experimental factors included two soil tillage systems and seven weed control methods in potato. Iron, copper and zinc in weeds were determined with the AAS method. The trace element content in weed dry matter before row closure of potato depended significantly on soil tillage methods (excluding Cu),...

  12. Origin of worldwide cultivated barley revealed by NAM-1 gene and grain protein content

    Directory of Open Access Journals (Sweden)

    Yonggang eWang

    2015-09-01

    Full Text Available The origin, evolution and distribution of cultivated barley provides powerful insights into the historic origin and early spread of agrarian culture. Here, population-based genetic diversity and phylogenetic analyses were performed to determine the evolution and origin of barley and how domestication and subsequent introgression have affected the genetic diversity and changes in cultivated barley on a worldwide scale. A set of worldwide cultivated and wild barleys from Asia and Tibet of China were analyzed using the sequences for NAM-1 gene and gene-associated traits-GPC (grain protein content. Our results showed Tibetan wild barley distinctly diverged from Near Eastern barley, and confirmed that Tibet is one of the origin and domestication centers for cultivated barley, and in turn supported a polyphyletic origin of domesticated barley. Comparison of haplotype composition among geographic regions revealed gene flow between Eastern and Western barley populations, suggesting that the Silk Road might have played a crucial role in the spread of genes. The GPC in the 118 cultivated and 93 wild barley accessions ranged from 6.73% to 12.35% with a mean of 9.43%. Overall, wild barley had higher averaged GPC (10.44% than cultivated barley. Two unique haplotypes (Hap2 and Hap7 caused by a base mutations (at position 544 in the coding region of the NAM-1 gene might have a significant impact on the GPC. SNPs and haplotypes of NAM-1 associated with GPC in barley could provide a useful method for screening GPC in barley germplasm. The Tibetan wild accessions with lower GPC could be useful for malt barley breeding

  13. Research of Cultivated Land Management Zones Based on the Evaluation of Farmland Productivity-Taking Qingzhou City,Shandong Province as an Example%基于县域耕地地力评价的耕地管理分区研究--以山东省青州市为例

    Institute of Scientific and Technical Information of China (English)

    姜纯; 董超; 李百红; 周光建; 苏诗雅

    2016-01-01

    Based on the GIS technology,appropriate evaluation factors were selected and the cultivated land in Qingzhou City was evaluated by the analytical hierarchy process,fuzzy evaluation and other methods.Using fuzzy C-means clustering method,study on delineation of management zones was performed,and the result showed that the overall quality of the cultivated land in the study area is high .Moreover,three optimal zones were determined according to the FPI and NCE index ,the result showed that the cultivated land fertility decreased from north to south ,greatly in-fluenced by terrain factors.Further delineation for subdistrict 1 was carried out by using fuzzy clustering method,giving six management zones. Therefore,more uniform internal partition management unit was obtained.The research provides a rational reference for management and utiliza-tion of the cultivated land.%基于GIS技术选取合适评价因子,运用层次分析、模糊评价等方法对青州市耕地进行定量化评价。利用模糊C均值聚类法对评价结果进行分区研究,揭示该市耕地质量整体较好;依据FPI和NCE指数确定最佳分区数目为3个,由北向南耕地地力依次降低,受地形因素影响较大;利用模糊聚类方法进一步对分区1进行划分,分为6个管理子区,得到了内部更为均衡的分区管理单元,为统一采取管理措施提供了有益的参考。

  14. Functional State Modelling of Cultivation Processes: Dissolved Oxygen Limitation State

    Directory of Open Access Journals (Sweden)

    Olympia Roeva

    2015-04-01

    Full Text Available A new functional state, namely dissolved oxygen limitation state for both bacteria Escherichia coli and yeast Saccharomyces cerevisiae fed-batch cultivation processes is presented in this study. Functional state modelling approach is applied to cultivation processes in order to overcome the main disadvantages of using global process model, namely complex model structure and a big number of model parameters. Alongwith the newly introduced dissolved oxygen limitation state, second acetate production state and first acetate production state are recognized during the fed-batch cultivation of E. coli, while mixed oxidative state and first ethanol production state are recognized during the fed-batch cultivation of S. cerevisiae. For all mentioned above functional states both structural and parameter identification is here performed based on experimental data of E. coli and S. cerevisiae fed-batch cultivations.

  15. 计量经济学教学模式的改革与能力培养%The Reform of the Teaching Methods for Econometrics and the Capacity Cultivation

    Institute of Scientific and Technical Information of China (English)

    孙文杰

    2015-01-01

    计量经济学是本科院校财经类或经管类专业的核心基础课程之一,对学生创新能力的培养和综合素质的提高具有十分重要的作用。本文围绕计量经济学教学中需要培养的关键能力,从培养学生独立思考的能力,运用恰当的计量模型对经济现象或经济问题进行抽象的能力以及比较和评价不同计量经济理论方法的能力等方面,提出计量经济学教学模式改革的对策和思路,为提高计量经济学的教学效果提供有益借鉴。%The econometrics is one of core basic courses of the major of the finance and economics or the economics and man-agement, which is most important for the cultivation of the stu-dents' innovation capacity and the improvement of the overall quality. Around the key capacity that should be acquired in teaching econometrics, the author analyzes the cultivation of the students' independent thinking capacity, the capacity of applying proper econometrics models to represent the economic phenome-na and economic problems and the capacity of comparing and e-valuating different econometrics theories and models, and puts forward the proposals and ideas for the reform of the teaching methods of econometrics in order to enhance the teaching effect of econometrics.

  16. Model-based methods for linkage analysis.

    Science.gov (United States)

    Rice, John P; Saccone, Nancy L; Corbett, Jonathan

    2008-01-01

    The logarithm of an odds ratio (LOD) score method originated in a seminal article by Newton Morton in 1955. The method is broadly concerned with issues of power and the posterior probability of linkage, ensuring that a reported linkage has a high probability of being a true linkage. In addition, the method is sequential so that pedigrees or LOD curves may be combined from published reports to pool data for analysis. This approach has been remarkably successful for 50 years in identifying disease genes for Mendelian disorders. After discussing these issues, we consider the situation for complex disorders where the maximum LOD score statistic shares some of the advantages of the traditional LOD score approach, but is limited by unknown power and the lack of sharing of the primary data needed to optimally combine analytic results. We may still learn from the LOD score method as we explore new methods in molecular biology and genetic analysis to utilize the complete human DNA sequence and the cataloging of all human genes.

  17. New ITF measure method based on fringes

    Science.gov (United States)

    Fang, Qiaoran; Liu, Shijie; Gao, Wanrong; Zhou, You; Liu, HuanHuan

    2016-01-01

    With the unprecedented developments of the intense laser and aerospace projects', the interferometer is widely used in detecting middle frequency indicators of the optical elements, which put forward very high request towards the interferometer system transfer function (ITF). Conventionally, the ITF is measured by comparing the power spectra of known phase objects such as high-quality phase step. However, the fabrication of phase step is complex and high-cost, especially in the measurement of large-aperture interferometer. In this paper, a new fringe method is proposed to measure the ITF without additional objects. The frequency was changed by adjusting the number of fringes, and the normalized transfer function value was measured at different frequencies. The ITF value measured by fringe method was consistent with the traditional phase step method, which confirms the feasibility of proposed method. Moreover, the measurement error caused by defocus was analyzed. The proposed method does not require the preparation of a step artifact, which greatly reduces the test cost, and is of great significance to the ITF measurement of large aperture interferometer.

  18. AN EVEN COMPONENT BASED FACE RECOGNITION METHOD

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    This paper presents a novel face recognition algorithm. To provide additional variations to training data set, even-odd decomposition is adopted, and only the even components (half-even face images) are used for further processing. To tackle with shift-variant problem,Fourier transform is applied to half-even face images. To reduce the dimension of an image,PCA (Principle Component Analysis) features are extracted from the amplitude spectrum of half-even face images. Finally, nearest neighbor classifier is employed for the task of classification. Experimental results on ORL database show that the proposed method outperforms in terms of accuracy the conventional eigenface method which applies PCA on original images and the eigenface method which uses both the original images and their mirror images as training set.

  19. LEVEL SET METHODS BASED ON DISTANCE FUNCTION

    Institute of Scientific and Technical Information of China (English)

    王德军; 唐云; 于洪川; 唐泽圣

    2003-01-01

    Some basic problems on the level set methods were discussed, such as the method used to preserve the distance function, the existence and uniqueness of solution for the level set equations. The main contribution is to prove that in a neighborhood of the initial zero level set, the level set equations with the restriction of the distance function have a unique solution, which must be the signed distance function with respect to the evolving surface. Some skillful approaches were used: Noticing that any solution for the original equation was a distance function, the original level set equations were transformed into a simpler alternative form. Moreover, since the new system was not a classical one, the system was transforned into an ordinary one, for which the implicit function method was adopted.

  20. Cultivo de Oncidium baueri Lindley (Orchidaceae) em substratos a base de coco Oncidium baueri Lindley (Orchidaceae) cultivation in coconut-based substrates

    OpenAIRE

    Adriane Marinho de Assis; Ricardo Tadeu Faria; Lilian Keiko Unemoto; Larissa Abgariani Colombo

    2008-01-01

    Entre os substratos utilizados no cultivo de orquídeas, a fibra de coco vem se destacando como promissor substituto do xaxim, material utilizado por muitos produtores e colecionadores de orquídeas, porém ameaçado de extinção. Objetivou-se, neste trabalho, foi avaliar a eficiência de substratos à base de coco, no cultivo da orquídea Oncidium baueri. As mudas foram cultivadas em vasos de polipropileno, permanecendo em viveiro com 50% de luminosidade. Os tratamentos constituíram-se de xaxim desf...

  1. Valuing Convertible Bonds Based on LSRQM Method

    Directory of Open Access Journals (Sweden)

    Jian Liu

    2014-01-01

    Full Text Available Convertible bonds are one of the essential financial products for corporate finance, while the pricing theory is the key problem to the theoretical research of convertible bonds. This paper demonstrates how to price convertible bonds with call and put provisions using Least-Squares Randomized Quasi-Monte Carlo (LSRQM method. We consider the financial market with stochastic interest rates and credit risk and present a detailed description on calculating steps of convertible bonds value. The empirical results show that the model fits well the market prices of convertible bonds in China’s market and the LSRQM method is effective.

  2. A numerical method based on probability theory

    Institute of Scientific and Technical Information of China (English)

    唐立; 邹捷中; 杨文胜

    2003-01-01

    By using the connections between Brownian family with drift and elliptic differential equations, an efficient probabilistic computing method is given. This method is applied to a wide-range Diriehlet problem. Detail analysis and deduction of solving the problem are offered. The stochastic representation of the solution to the problem makes a 3-dimensional problem turned into a 2-dimensional problem. And an auxiliary ball is constructed. The strong Markov property and the joint distributions of the time and place of hitting spheres for Brownian family with drift are employed. Finally, good convergence of the numerical solution to the problem over domain with arbitrary boundary is obtained.

  3. Mathematic Teaching Strategy Based on Teamwork Spirit Cultivation%基于合作意识培养的数学教学策略探析

    Institute of Scientific and Technical Information of China (English)

    雷兴辉

    2011-01-01

    针对以就业为导向的职业院校数学教学,探讨在教学中培养学生合作意识和合作能力的策略,以期为学生就业做好数学方面的准备.%Aiming at employment-oriented mathematic teaching in vocational college, the article explored the strategy of cultivating students' teamwork spirit and team work ability in teaching, in order to prepare for students' employment.

  4. Glass bead cultivation of fungi

    DEFF Research Database (Denmark)

    Droce, Aida; Sørensen, Jens Laurids; Giese, H.;

    2013-01-01

    Production of bioactive compounds and enzymes from filamentous fungi is highly dependent on cultivation conditions. Here we present an easy way to cultivate filamentous fungi on glass beads that allow complete control of nutrient supply. Secondary metabolite production in Fusarium graminearum...

  5. Cultivation of microalgae in industrial wastewaters

    DEFF Research Database (Denmark)

    van Wagenen, Jonathan Myerson

    anaerobic treatment methods would result in effluent containing dissolved organic molecules suitable for algae species that have the ability to grow as mixo- or heterotrophs. Chlorella sorokiniana was cultivated in a lab scale photobioreactor under daily light dark cycles and various timing strategies were...... tested for adding acetate at concentrations that can be obtained in waste streams of 1 – 2 g L-1. The results showed that the fastest growth occurred when adding the acetate at night (cyclic autotrophy/heterotrophy). However adding the acetate during the day (mixotrophty) also improved growth compared...... cultivation medium. The screening method developed will reduce the cost of identifying the best conditions to test at lab scale. The D-stat method offers a way to identify the best conditions for biomass production and nutrient removal. Various options for heterotrophic and mixotrophic utilization of waste...

  6. HMM-Based Gene Annotation Methods

    Energy Technology Data Exchange (ETDEWEB)

    Haussler, David; Hughey, Richard; Karplus, Keven

    1999-09-20

    Development of new statistical methods and computational tools to identify genes in human genomic DNA, and to provide clues to their functions by identifying features such as transcription factor binding sites, tissue, specific expression and splicing patterns, and remove homologies at the protein level with genes of known function.

  7. Development and application of a quantitative method based on LC-QqQ MS/MS for determination of steviol glycosides in Stevia leaves.

    Science.gov (United States)

    Molina-Calle, M; Sánchez de Medina, V; Delgado de la Torre, M P; Priego-Capote, F; Luque de Castro, M D

    2016-07-01

    Stevia is a currently well-known plant thanks to the presence of steviol glycosides, which are considered as sweeteners obtained from a natural source. In this research, a method based on LC-MS/MS by using a triple quadrupole detector was developed for quantitation of 8 steviol glycosides in extracts from Stevia leaves. The ionization and fragmentation parameters for selected reaction monitoring were optimized. Detection and quantitation limits ranging from 0.1 to 0.5ng/mL and from 0.5 to 1ng/mL, respectively, were achieved: the lowest attained so far. The steviol glycosides were quantified in extracts from leaves of seven varieties of Stevia cultivated in laboratory, greenhouse and field. Plants cultivated in field presented higher concentration of steviol glycosides than those cultivated in greenhouse. Thus, the way of cultivation clearly influences the concentration of these compounds. The inclusion of branches together with leaves as raw material was also evaluated, showing that this inclusion modifies, either positively or negatively, the concentration of steviol glycosides.

  8. Development and application of a quantitative method based on LC-QqQ MS/MS for determination of steviol glycosides in Stevia leaves.

    Science.gov (United States)

    Molina-Calle, M; Sánchez de Medina, V; Delgado de la Torre, M P; Priego-Capote, F; Luque de Castro, M D

    2016-07-01

    Stevia is a currently well-known plant thanks to the presence of steviol glycosides, which are considered as sweeteners obtained from a natural source. In this research, a method based on LC-MS/MS by using a triple quadrupole detector was developed for quantitation of 8 steviol glycosides in extracts from Stevia leaves. The ionization and fragmentation parameters for selected reaction monitoring were optimized. Detection and quantitation limits ranging from 0.1 to 0.5ng/mL and from 0.5 to 1ng/mL, respectively, were achieved: the lowest attained so far. The steviol glycosides were quantified in extracts from leaves of seven varieties of Stevia cultivated in laboratory, greenhouse and field. Plants cultivated in field presented higher concentration of steviol glycosides than those cultivated in greenhouse. Thus, the way of cultivation clearly influences the concentration of these compounds. The inclusion of branches together with leaves as raw material was also evaluated, showing that this inclusion modifies, either positively or negatively, the concentration of steviol glycosides. PMID:27154673

  9. Cultivating Technological Innovation in the Mind

    Directory of Open Access Journals (Sweden)

    PROF ANYATA BENEDICT U.(Ph.D

    2015-11-01

    Full Text Available Cultivating innovation in the mind is imperative to successfully move technology to the next level. Technology is growing rapidly in developed countries and has almost reached its peak. Developing countries and continents like Africa on the other hand are now seen as a breeding ground to explore in technological innovation. Researchers and entrepreneurs are shifting base to Africa where they can achieve maximum profit resulting from under development of the continent. Reefs, city and web are used to explain the best way to cultivate innovation. The city and web are such engines of new innovation because both environments are powerfully suited for the creation, diffusion and adoption of good ideas. The seven keys of cultivating innovation include tropical humidity, illiteracy, poor power surge of electronic use for research, good concept of the liquid networks, slow hunches, serendipity, acceptance of error in cultivating innovation, Exaptation, coffee breaks, application of programming interface and the use of quadrant as a tool. These keys are recommended to be put to use by every individual who has the mindset of driving technological innovation to the next level.

  10. String-Based Methods in Perturbative Gravity

    OpenAIRE

    Bern, Z.; Dunbar, D.C.; Shimada, T.

    1993-01-01

    String theory implies a relatively modest growth in computational complexity for perturbative gravity calculations as compared to gauge theory calculations, contrary to field theory expectations. An explicit string-based calculation, which would be extremely difficult using conventional techniques, is presented to illustrate this.

  11. On Cultivating Students' Motivation in Second Language Acquisition

    Institute of Scientific and Technical Information of China (English)

    沈小平

    2009-01-01

    This paper attempts to explore the cultivation of motivation in second language acquisition based on shedding new light on the definition, the importance, and the classification of learners' motivation in second language acquisition.

  12. Labor Cost Analysis for Pome Production in Different Cultivation Modes in Hebei Province

    Institute of Scientific and Technical Information of China (English)

    Shaoyan; ZHOU; Yu; HUANG; Cui; YAO; Runhang; LU; Xingfang; LIU

    2013-01-01

    Taking the traditional fruit pear as the example, this paper analyzes labor cost for pome production in Hebei Province, based on the representative cases and the research of pome production in different cultivation modes. Firstly, it conducts cost analysis for medium-density pome production in Xinji City, focusing on the comparison of the costs for the main production labor in standard thin planting mode and dwarf close planting mode. According to the research results, labor cost has a great influence on the total production cost of pome. The methods to reduce labor cost include: adopting dwarfing rootstock close planting and intensively efficient pome cultivation method; simplifying the pruning method when matching up the shape of tree; improving soil by the methods of natural grasses and addition of organic materials, and increasing mechanized operation.

  13. IN-SITU DETERMINATION OF SEDIMENT OXYGEN DEMAND IN CULTIVATION PONDS

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    This paper presents a method for in-situ determining sediment oxygen demand (SOD) in cultivation pond. This method based on sediment surface structure, temperature, and other determining conditions like those in shrimp cultivation environments, overcomes defects of old methods and provides more accurate estimation of SOD's effect on dissolved oxygen in culture waters. Our experiment shows that the sediment surface structure and temperature had important effect on SOD in culture water. Different SOD values were derived from different parts of oxygen consumption curves of sediment, because the curves were not linear. According to the oxygen consumption curves of sediment and saturated DO in culture water, it was thought more suitable to calculate SOD with dissolved oxygen reduction from 5.0 to 2.0 mg/l. This method to determine the SOD of shrimp ponds yielded satisfactory results.

  14. High throughput Single-cell Cultivation on Microfluidic Streak Plates

    OpenAIRE

    Jiang, Cheng-Ying; Dong, Libing; Zhao, Jian-Kang; Hu, Xiaofang; Shen, Chaohua; Qiao, Yuxin; Zhang, Xinyue; Wang, Yapei; Ismagilov, Rustem F.; Liu, Shuang-Jiang; Du, Wenbin

    2016-01-01

    This paper describes the microfluidic streak plate (MSP), a facile method for high-throughput microbial cell separation and cultivation in nanoliter sessile droplets. The MSP method builds upon the conventional streak plate technique by using microfluidic devices to generate nanoliter droplets that can be streaked manually or robotically onto petri dishes prefilled with carrier oil for cultivation of single cells. In addition, chemical gradients could be encoded in the droplet array for compr...

  15. 基于四网融合的产业升级与新兴产业培育研究%Industry Upgrade and New Industry Cultivation Based on Four Synergetic Networks

    Institute of Scientific and Technical Information of China (English)

    孙德忠; 周荣; 喻登科

    2014-01-01

    The abundant connotations of value network ,knowledge network ,social network and internet of things are tried out ,and their reciprocities are analyzed ,and then the four networks synchronized mechanism is researched based on the two-two fusion .The industry upgrade and new industry cultivation mechanism based on four synergetic networks are dis-cussed from essence ,mechanism and process .Two industry upgrade modes including grandness and upgrade ,and four new industry cultivation modes including gap padding ,network complementary ,service intensifying and efficiency leading are put forward .These can provide a new development theory for industry upgrade and new industry cultivation of China .%提炼出了价值网、知识网、社会网和物联网的丰富内涵,剖析了它们之间的相互作用关系,从两两融合的角度研究了四网融合机理。进而从本质、机制和过程3个层面探讨了基于四网融合的产业升级与新兴产业培育机理,提出了四网融合下壮大式和升级式两种产业升级模式,以及缝隙填补、网络互补、服务强化、效率领先等4种新兴产业培育模式,为我国产业升级和新兴产业培育提供了一种崭新的发展理念。

  16. An ICA based method for texture recognition

    OpenAIRE

    Fournel, Thierry; Becker, Jean-Marie; Daniela COLTUC; Yann BOUTANT

    2006-01-01

    The method proposed in this paper uses the Independent Component Analysis (ICA) for an application of unsupervised recognition of textures. The analysed texture is modelled by a weighted sum of almost statistically independent random signals that are extracted with FastICA algorithm. Each resulting signal is described by its negentropy, more precisely, by one of the approximations used by FastICA algorithm. The approximated negentropies are sorted into descending order and represented by a cu...

  17. 基于碳循环的黄淮海平原耕地固碳功能研究%Carbon sequestration function of cultivated land use system based on the carbon cycle for the Huang-Huai-Hai Plain

    Institute of Scientific and Technical Information of China (English)

    陈丽; 郝晋珉; 王峰; 尹钰莹; 高阳; 段文凯; 杨君

    2016-01-01

    Cultivated land carbon sequestration as an effective supplement to the terrestrial ecosystem is recognized;however,research on the carbon sequestration function of cultivated land has been conducted from the perspective of ecology.For example,carbon uptake and carbon release of vegetation and arable soil and carbon balance in agro-ecosystems is often a focus and there is a lack of research into the carbon sequestration of cultivated land from the viewpoint of land science.Here we built a basic research framework for the carbon sequestration function of cultivated land use for the Huang-Huai-Hai Plain based on the carbon cycle process of cultivated land use system theory.Then the carbon sequestration function was evaluated from three aspects of carbon sequestration ability,carbon sequestration efficiency and carbon sequestration dominance.The results suggest that the carbon sequestration function of cultivated land use can be known from four aspects:natural carbon sequestration ability,ideal carbon sequestration ability,practical carbon sequestration ability and regional carbon sequestration ability.The carbon sequestration of cultivated land use systems of the Huang-Huai-Hai Plain is a carbon sink.The carbon sequestration efficiency and carbon sequestration dominance degree are 2.5 and 0.85 respectively.The carbon sequestration function of cultivated land use system plays an irreplaceable role on the Huang-Huai-Hai Plain,which should be a prominent function in this area.This study provides a new way of thinking about ecological function theory research for cultivated land,and support for promoting the ecological benefits of cultivated land,mitigating climate warming,and administering cultivated land ecology interregional compensation.%耕地固碳作为陆地主要生态系统固碳的有效补充,越来越引起学术界的广泛关注.但是,目前耕地固碳功能研究多从生态学角度研究耕地植被、土壤等碳吸收、碳释放

  18. 高职酒店管理专业学生职业素质培养研究-基于中原经济区建设背景%On the Cultivation of Occupational Qualities of Hotel Management Students in Vocational Colleges:Based on the Construction Background of the Central Plains Economic Zone

    Institute of Scientific and Technical Information of China (English)

    张志强

    2014-01-01

    在中原经济区的建设大背景下,酒店业迎来了新的发展机遇,但是酒店管理专业人才的职业素养现状与人才需求之间仍有较大差距。为培养酒店高素质服务型人才,助推中原经济区建设,针对目前酒店业人才需求现状和高职人才培养现状,从人才培养定位、课程与教学体系改革、校企合作等方面提出了高职酒店管理专业学生职业素质培养的途径。%In the background of the construction of the Central Plains Economic Zone, the hotel business is facing new opportunities for development, but there is still a wide gap between the hotel management students’ professionalism and talent demand of hotel management. In order to cultivate high-quality hotel services professionals, boosting the economic development of the Central Plains region, based on the current hotel industry demand and the present situation of higher vocational talent training, the article puts forward higher vocational hotel management professional quality development methods to cultivate students’ professional quality, which range from talents cultivation orientation, curriculum and teaching system reform, to university-enterprise cooperation and so on.

  19. On the Cultivation of Occupational Qualities of Hotel Management Students in Vocational Colleges:Based on the Construction Background of the Central Plains Economic Zone%高职酒店管理专业学生职业素质培养研究-基于中原经济区建设背景

    Institute of Scientific and Technical Information of China (English)

    张志强

    2014-01-01

    In the background of the construction of the Central Plains Economic Zone, the hotel business is facing new opportunities for development, but there is still a wide gap between the hotel management students’ professionalism and talent demand of hotel management. In order to cultivate high-quality hotel services professionals, boosting the economic development of the Central Plains region, based on the current hotel industry demand and the present situation of higher vocational talent training, the article puts forward higher vocational hotel management professional quality development methods to cultivate students’ professional quality, which range from talents cultivation orientation, curriculum and teaching system reform, to university-enterprise cooperation and so on.%在中原经济区的建设大背景下,酒店业迎来了新的发展机遇,但是酒店管理专业人才的职业素养现状与人才需求之间仍有较大差距。为培养酒店高素质服务型人才,助推中原经济区建设,针对目前酒店业人才需求现状和高职人才培养现状,从人才培养定位、课程与教学体系改革、校企合作等方面提出了高职酒店管理专业学生职业素质培养的途径。

  20. 综合运用多种方法,有效培养学生应用写作语言风格%Comprehensive use of various methods, effectively cultivate students' practical writing language style

    Institute of Scientific and Technical Information of China (English)

    王颖

    2013-01-01

    In the application writing teaching, the language style is the difficult point of teaching, its reason is no concept of applied writing language style of students in learning, in order to solve this problem, in this paper, the theory of implicit learning and explicit learning are introduces into the teaching, and put forward comprehensive use of reading method, comparative method and practice method in teaching links, in order to cultivate students' practical writing language style.%  在应用写作的教学中,应用文的语言风格是教学的难点,其原因是学生在学习中往往对应用写作的语言风格没有概念,针对这一问题,本文将内隐学习和外显学习的相关理论引入教学,并提出在教学的前、中、后等环节,综合运用阅读法、比较法和实践训练法,以期有效培养学生的应用写作语言风格。

  1. Teaching Reform and Practice of Nursing Specialty Courses Based on Cultivation of Humanistic Caring Ability%以人文关怀能力培养为本位的护理专业课教学改革与实践

    Institute of Scientific and Technical Information of China (English)

    贾娟娟; 汪洪杰; 黄弋冰

    2013-01-01

    目的探讨以人文关怀能力培养为本位的护理专业课教学改革的效果。方法采用随机整群抽样,从某校二年级专科护生中抽取8个班作为研究对象,按随机数字表法分为观察组和对照组。对照组在护理专业课教学中遵循传统教学,观察组以人文关怀能力培养为本位对护理专业课的教学内容、教学方法及考核方式进行改革。临床实习中期分别对两组学生及其临床带教老师应用护生人文关怀能力量表和自行设计的调查问卷进行调查,评价实施效果。结果学期结束后护生自评及临床带教老师评价均显示观察组人文关怀能力高于对照组(P<0.05)。结论以人文关怀能力培养为本位的护理专业课教学改革有助于提高护生的人文关怀能力。%Objective To evaluate the effect of teaching reform of nursing specialty courses based on the cultivation of humanistic caring ability. Methods By cluster sampling, eight classes of sophomore nursing students were randomly divided into control group and experiment group. Traditional teaching method was applied in control group while in experiment group, based on the cultivation of humanistic caring ability, a new teaching method including reformed teaching content, teaching method and evaluation mode was used. In mid-term of clinical practice, nursing student humanistic caring ability scale and a self-designed questionnaire were used to evaluate the effect. Results Both nursing students’ self-evaluation and the evaluation on clinical teachers showed students in experiment group presented higher humanistic caring ability (P<0.05). Conclusion Nursing specialty courses based on the cultivation of humanistic caring ability help to enhance nursing students ’ humanistic caring ability.

  2. Knowledge-based methods for control systems

    International Nuclear Information System (INIS)

    This thesis consists of three projects which combine artificial intelligence and control. The first part describes an expert system interface for system identification, using the interactive identification program Idpac. The interface works as an intelligent help system, using the command spy strategy. It contains a multitude of help system ideas. The concept of scripts is introduced as a data structure used to describe the procedural part of the knowledge in the interface. Production rules are used to represent diagnostic knowledge. A small knowledge database of scripts and rules has been developed and an example run is shown. The second part describes an expert system for frequency response analysis. This is one of the oldest and most widely used methods to determine the dynamics of a stable linear system. Though quite simple, it requires knowledge and experience of the user, in order to produce reliable results. The expert system is designed to help the user in performing the analysis. It checks whether the system is linear, finds the frequency and amplitude ranges, verifies the results, and, if errors should occur, tries to give explanation and remedies for them. The third part describes three diagnostic methods for use with industrial processes. They are measurement validation, i.e., consistency checking of sensor and measurement values using any redundancy of instrumentation; alarm analysis, i.e. analysis of multiple alarm situations to find which alarms are directly connected to primary faults and which alarms are consequential effects of the primary ones; and fault diagnosis, i.e., a search for the causes of and remedies for faults. The three methods use multilevel flow models, (MFM), to describe the target process. They have been implemented in the programming tool G2, and successfully tested on two small processes. (164 refs.) (au)

  3. Scope-Based Method Cache Analysis

    DEFF Research Database (Denmark)

    Huber, Benedikt; Hepp, Stefan; Schoeberl, Martin

    2014-01-01

    , as it requests memory transfers at well-defined instructions only. In this article, we present a new cache analysis framework that generalizes and improves work on cache persistence analysis. The analysis demonstrates that a global view on the cache behavior permits the precise analyses of caches which are hard......The quest for time-predictable systems has led to the exploration of new hardware architectures that simplify analysis and reasoning in the temporal domain, while still providing competitive performance. For the instruction memory, the method cache is a conceptually attractive solution...

  4. Proposal for Scrambled Method based on NTRU

    Directory of Open Access Journals (Sweden)

    Ahmed Tariq Sadiq

    2015-08-01

    Full Text Available Scrambling is widely used to protect the security of data files such as text, image, video or audio files; however, it is not the most efficient method to protect the security of the data files. This article uses NTRU public key cryptosystem to increase the robustness of scrambling of sound files. In this work, we convert the sound file into text, and then scramble it in the following way: first, we encrypt the header of the sound file then, scramble the data of the file after the header in three stages. In each stage we scramble the data of the sound file and keep the original order of data in an array then, the three arrays are encrypted by the sender and sent with the encrypted header to the receiver in one file, while the scrambled data of the sound file is sent to the receiver in another file. We have tested the proposed method on several sound files; the results show that the time of encryption and decryption is reduced to approximately one-third, or less, compared to encrypting the file using NTRU.

  5. Inculcation Method of Character Education Based on Personality Types Classification in Realizing Indonesia Golden Generation

    Science.gov (United States)

    Sunarto, M. J. Dewiyani; Sagirani, Tri

    2014-01-01

    "The rise of Indonesia Golden Generation" is the theme of National Education Day in 2012. In an effort to create a golden generation; education must be interpreted as a complex problem, in particular the cultivation of character education that was originally using indoctrination method. Given the shifting of the changing times,…

  6. Classification and Gradation of Cultivated Land Quality in Bishan County of Chongqing, China

    Institute of Scientific and Technical Information of China (English)

    SHAO Jing'an; GE Xiaofeng; WEI Chaofu; XIE Deti

    2007-01-01

    The conflicts among food security, economic development and ecological protection are the "sticking point"of undeveloped southwestern mountainous areas of China. The objectives of this study are to identify appropriate integrated indicators influencing the classification and gradation of cultivated land quality in the southwestern mountainous area of China based on semi-structure interview, and to promote the monitoring of cultivated land quality in this region.Taking Bishan County of Chongqing as a study case, the integrated indicators involve the productivity, protection, acceptability, and stability of cultivated land. The integrated indicators accord with the characteristics of land resources and human preference in southwestern mountainous area of China. In different agricultural zones, we emphasize different indicators, such as emphasizing productivity, stabilization and acceptability in low hilly and plain agricultural integrative zone (LHP-AIZ), protection, productivity and stability in low mountain and hill agro-forestry ecological zone (LMH-AEZ), and acceptability in plain outskirts integrative agricultural zone (PO-IAZ), respectively. The pronounced difference of classification and gradation of cultivated land, regardless of inter-region or intra-region, is observed, with the reducible rank from PO-IAZ, LHP-AIZ to LMH-AEZ. Research results accord with the characteristics of assets management and intensive utilization of cultivated land resources in the southwestern mountainous area of China.Semi-structure interview adequately presents the principal agent of farmers in agricultural land use and rural land market.This method is very effective and feasible to obtain data of the quality of cultivated land in the southwestern mountainous area of China.

  7. Triptycene-based dianhydrides, polyimides, methods of making each, and methods of use

    KAUST Repository

    Ghanem, Bader

    2015-12-30

    A triptycene-based monomer, a method of making a triptycene-based monomer, a triptycene-based aromatic polyimide, a method of making a triptycene- based aromatic polyimide, methods of using triptycene-based aromatic polyimides, structures incorporating triptycene-based aromatic polyimides, and methods of gas separation are provided. Embodiments of the triptycene-based monomers and triptycene-based aromatic polyimides have high permeabilities and excellent selectivities. Embodiments of the triptycene-based aromatic polyimides have one or more of the following characteristics: intrinsic microporosity, good thermal stability, and enhanced solubility. In an exemplary embodiment, the triptycene-based aromatic polyimides are microporous and have a high BET surface area. In an exemplary embodiment, the triptycene-based aromatic polyimides can be used to form a gas separation membrane.

  8. Deforestation and cultivation mobilize mercury from topsoil.

    Science.gov (United States)

    Gamby, Rebecca L; Hammerschmidt, Chad R; Costello, David M; Lamborg, Carl H; Runkle, James R

    2015-11-01

    Terrestrial biomass and soils are a primary global reservoir of mercury (Hg) derived from natural and anthropogenic sources; however, relatively little is known about the fate and stability of Hg in the surface soil reservoir and its susceptibility to change as a result of deforestation and cultivation. In southwest Ohio, we measured Hg concentrations in soils of deciduous old- and new-growth forests, as well as fallow grassland and agricultural soils that had once been forested to examine how, over decadal to century time scales, man-made deforestation and cultivation influence Hg mobility from temperate surface soils. Mercury concentrations in surficial soils were significantly greater in the old-growth than new-growth forest, and both forest soils had greater Hg concentrations than cultivated and fallow fields. Differences in Hg:lead ratios between old-growth forest and agricultural topsoils suggest that about half of the Hg lost from deforested and cultivated Ohio soils may have been volatilized and the other half eroded. The estimated mobilization potential of Hg as a result of deforestation was 4.1 mg m(-2), which was proportional to mobilization potentials measured at multiple locations in the Amazon relative to concentrations in forested surface soils. Based on this relationship and an estimate of the global average of Hg concentrations in forested soils, we approximate that about 550 M mol of Hg has been mobilized globally from soil as a result of deforestation during the past two centuries. This estimate is comparable to, if not greater than, the amount of anthropogenic Hg hypothesized by others to have been sequestered by the soil reservoir since Industrialization. Our results suggest that deforestation and soil cultivation are significant anthropogenic processes that exacerbate Hg mobilization from soil and its cycling in the environment.

  9. Deforestation and cultivation mobilize mercury from topsoil.

    Science.gov (United States)

    Gamby, Rebecca L; Hammerschmidt, Chad R; Costello, David M; Lamborg, Carl H; Runkle, James R

    2015-11-01

    Terrestrial biomass and soils are a primary global reservoir of mercury (Hg) derived from natural and anthropogenic sources; however, relatively little is known about the fate and stability of Hg in the surface soil reservoir and its susceptibility to change as a result of deforestation and cultivation. In southwest Ohio, we measured Hg concentrations in soils of deciduous old- and new-growth forests, as well as fallow grassland and agricultural soils that had once been forested to examine how, over decadal to century time scales, man-made deforestation and cultivation influence Hg mobility from temperate surface soils. Mercury concentrations in surficial soils were significantly greater in the old-growth than new-growth forest, and both forest soils had greater Hg concentrations than cultivated and fallow fields. Differences in Hg:lead ratios between old-growth forest and agricultural topsoils suggest that about half of the Hg lost from deforested and cultivated Ohio soils may have been volatilized and the other half eroded. The estimated mobilization potential of Hg as a result of deforestation was 4.1 mg m(-2), which was proportional to mobilization potentials measured at multiple locations in the Amazon relative to concentrations in forested surface soils. Based on this relationship and an estimate of the global average of Hg concentrations in forested soils, we approximate that about 550 M mol of Hg has been mobilized globally from soil as a result of deforestation during the past two centuries. This estimate is comparable to, if not greater than, the amount of anthropogenic Hg hypothesized by others to have been sequestered by the soil reservoir since Industrialization. Our results suggest that deforestation and soil cultivation are significant anthropogenic processes that exacerbate Hg mobilization from soil and its cycling in the environment. PMID:26100725

  10. Adaptive Mixture Methods Based on Bregman Divergences

    CERN Document Server

    Donmez, Mehmet A; Kozat, Suleyman S

    2012-01-01

    We investigate adaptive mixture methods that linearly combine outputs of $m$ constituent filters running in parallel to model a desired signal. We use "Bregman divergences" and obtain certain multiplicative updates to train the linear combination weights under an affine constraint or without any constraints. We use unnormalized relative entropy and relative entropy to define two different Bregman divergences that produce an unnormalized exponentiated gradient update and a normalized exponentiated gradient update on the mixture weights, respectively. We then carry out the mean and the mean-square transient analysis of these adaptive algorithms when they are used to combine outputs of $m$ constituent filters. We illustrate the accuracy of our results and demonstrate the effectiveness of these updates for sparse mixture systems.

  11. APPLICATION DIMENSIONAL AND SIMILARITY THEORY IN DETERMINING THE PARAMETERS AND OPERATING MODES OF SOIL CULTIVATING MACHINES

    Directory of Open Access Journals (Sweden)

    Shhirov V. N.

    2015-06-01

    Full Text Available The article presents a study of parameters and modes of operation of machines for soil cultivation. In determining the parameters and modes of operation of machinery for tillage we have applied the theory of similarity and dimensions of physical quantities. We have obtained the regularities of disclosing the relationship of the parameters from the medium to the energy characteristics of the process. As the initial data we used test protocols of machines for soil cultivation (Central - Black Earth, Kubanskaya, Sibirskaya, of North - Caucasion MIS, RosNIITiM : KPI - 3.8, AРC - 3.9, AKV - 4, AKM - 6 - V, AMP - 4 APC - 4 A, AРC - 10 APR - 4.4, APU - 6.5 APSH - 6 , CNC - 6.0, CSТ - 3.8, APC - 4. We defined the formula оf dimension parameters and modes of operation of machines for soil cultivation and properties of soil (traction resistance, depth, width, speed, hardness of the soil, acceleration. Based on dimension theory we have received similarity criteria. Based on the correlation analysis and the least squares method we determined the nature of addiction and the coefficients for it. We have also received a graph for determining the operating modes and parameters of machines for soil cultivation

  12. Gradient-based methods for sparse recovery

    CERN Document Server

    Hager, William; Zhang, Hongchao

    2009-01-01

    The convergence rate is analyzed for the SpaSRA algorithm (Sparse Reconstruction by Separable Approximation) for minimizing a sum $f (\\m{x}) + \\psi (\\m{x})$ where $f$ is smooth and $\\psi$ is convex, but possibly nonsmooth. It is shown that if $f$ is convex, then the error in the objective function at iteration $k$, for $k$ sufficiently large, is bounded by $a/(b+k)$ for suitable choices of $a$ and $b$. Moreover, if the objective function is strongly convex, then the convergence is $R$-linear. An improved version of the algorithm based on a cycle version of the BB iteration and an adaptive line search is given. The performance of the algorithm is investigated using applications in the areas of signal processing and image reconstruction.

  13. The Origin of Flooded Rice Cultivation

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    , slow decline of soil fertility, availability of minerals, and resulting in high yield per unit area, which have collectively attained the highly productive cereal cultivation in the warm and humid region. Rice cultivation in marsh is also favorable to raise fish culture, both of which constituted a nutritionally balanced base. Development of irrigation technology to construct flooded farms gave strong bases for stable rice-cultivating society, which in the end formulated the rise of ancient kingdoms of Yue and Wu in China in BC 6th -5th centuries. They were direct descendents of those people who had developed the unique rice cultivation from the era of Hemudu culture, which is dated back to 5 000 BC. Their movement to the south is considered to have established rice-cultivating communities in South China and Southeast Asia, while to the north it transferred the rice-based technology to ancient Korea and Japan and had established there a base for a civilized society.

  14. 案例教学法在医德修养教学中的应用%Application of Case Teaching Method in the Cultivation of Medical Ethics

    Institute of Scientific and Technical Information of China (English)

    李东霞

    2015-01-01

    Case teaching methodology is an instructive, practical, lfexible and interesting approach. It is suitable for application in the medical ethics education program. There are still some problems in case teaching, such as inadequate understanding of teaching methods, improperly selected cases and implementation method which does not reach the goal, etc. Methods solving these problems include deepened understanding of case teaching, scientifically selected cases, vividly demonstration, generalization and summarization. Case teaching methodology is a new and suitable approach for the medical ethics education.%案例教学法具有启发性、实践性、灵活性和趣味性等特点,适合应用于医德修养教学中。目前案例教学中还存在一些问题,如教师对案例教学的认识不足,案例选择不当,实施方法不到位等。解决的对策可以有加深教师对案例教学的认识,科学筛选案例,生动展现和归纳总结等方法。案例教学是适合医德教育特点的一种全新的教学方法。

  15. Enzyme controlled glucose auto-delivery for high cell density cultivations in microplates and shake flasks

    Directory of Open Access Journals (Sweden)

    Casteleijn Marco G

    2008-11-01

    Full Text Available Abstract Background Here we describe a novel cultivation method, called EnBase™, or enzyme-based-substrate-delivery, for the growth of microorganisms in millilitre and sub-millilitre scale which yields 5 to 20 times higher cell densities compared to standard methods. The novel method can be directly applied in microwell plates and shake flasks without any requirements for additional sensors or liquid supply systems. EnBase is therefore readily applicable for many high throughput applications, such as DNA production for genome sequencing, optimisation of protein expression, production of proteins for structural genomics, bioprocess development, and screening of enzyme and metagenomic libraries. Results High cell densities with EnBase are obtained by applying the concept of glucose-limited fed-batch cultivation which is commonly used in industrial processes. The major difference of the novel method is that no external glucose feed is required, but glucose is released into the growth medium by enzymatic degradation of starch. To cope with the high levels of starch necessary for high cell density cultivation, starch is supplied to the growing culture suspension by continuous diffusion from a storage gel. Our results show that the controlled enzyme-based supply of glucose allows a glucose-limited growth to high cell densities of OD600 = 20 to 30 (corresponding to 6 to 9 g l-1 cell dry weight without the external feed of additional compounds in shake flasks and 96-well plates. The final cell density can be further increased by addition of extra nitrogen during the cultivation. Production of a heterologous triosphosphate isomerase in E. coli BL21(DE3 resulted in 10 times higher volumetric product yield and a higher ratio of soluble to insoluble product when compared to the conventional production method. Conclusion The novel EnBase method is robust and simple-to-apply for high cell density cultivation in shake flasks and microwell plates. The

  16. A study on the comparison of antioxidant effects among wild ginseng, cultivated wild ginseng, and cultivated ginseng extracts

    Directory of Open Access Journals (Sweden)

    Hae Young, Jang

    2008-09-01

    Full Text Available Objective : The objective of this study was to compare the antioxidant effects among wild ginseng, cultivated wild ginseng, and ginseng extracts. Methods : In vitro antioxidant activities were examined by total antioxidant capacity (TAC, oxygen radical scavenging capacity(ORAC, total phenolic content, 1, 1-Diphenyl-2-picrylhydrazyl (DPPH radical scavenging activity, inhibition of induced lipid peroxidation using liver mitochondria, reactive oxygen species(ROS scavenging effect using 2’, 7’-dichlorofluorescein(DCF fluorescence. Results : 1. TAC of 1.5 and 3.75 mg extracts was highest in cultivated wild ginseng, followed by wild ginseng and lowest in ginseng. 2. ORAC of 2, 10, and 20 μg extracts was highest in cultivated wild ginseng, followed by wild ginseng and lowest in ginseng. 3. Total phenolic content of 0.375, 0.938, and 1.875 mg extracts was highest in cultivated wild ginseng, followed by wild ginseng and lowest in ginseng. 4. DPPH(1, 1 -Diphenyl-2-picrylhydrazyl scavenging activity between wild ginseng and cultivated wild ginseng did not differ significantly (p>0.05. 5. Induced lipid peroxidation, measured by TBARS concentration in solution containing rat liver mitochondria incubated in the presence of FeSO4/ascorbic acid was inhibited as amounts of wild ginseng, cultivated wild ginseng, and ginseng extracts increased. TBARS concentration of ginseng extracts were significantly (p<0.05 higher than wild ginseng or cultivated wild ginseng extracts. 6. DCF fluorescence intensity was decreased as concentrations of wild ginseng, cultivated wild ginseng, and ginseng extracts increased, demonstrating that ROS generation was inhibited in a concentrationdependent manner. Conclusions : In summary, the results of this study demonstrate that cultivated wild ginseng extracts had similar antioxidant activities to wild ginseng extracts and greater that of cultivated ginseng extracts.

  17. 特色专业建设培养高素质创新型采矿工程专业人才%Cultivate High .Quality Creative Mining Engineering Talents Based on the Construction of Professional Characteristics

    Institute of Scientific and Technical Information of China (English)

    康志强

    2012-01-01

    合理确定采矿工程特色专业建设目标,是采矿工程专业建设首先必须解决的问题。创新人才的培养是采矿工程学科得以存在和发展的关键,对于采矿工程学科具有重要的意义。为了培养出具有采矿特色的"基础实、知识面宽、实践能力强、综合素质高"的高素质创新型采矿工程专业人才,从完善人才培养方案,加强师资队伍建设,改革教学内容及课程体系,提高实践教学质量等方面,阐述了以采矿工程特色专业建设点的建设为依托,进一步完善高素质创新型人才培养体系的方法和措施,从而使学生的综合素质和创新能力得到较大程度的提高。%First of all, mining engineering construction must rationally determine the characteristics of professional development goals of mining engineering. Innovative talents training is the key of existence and development of safe- ty engineering discipline, which is of great significance for mining engineering discipline. In order to train with the characteristics of mining engineering, "based on fact, know/edge breadth, practical ability, and comprehensive high quality" high-quality creative mining engineering talents. The paper based on the construction of mining engineering professional characteristics development points from perfecting training plan strengthening the teaching staff, teach- ing contents and curriculum reform, improvement of education quality, relying on further improving the methods and measures of cultivating the high-quality creative talents. So that the students overall quality and level of creative has been greatly improved.

  18. Theory-Based Lexicographical Methods in a Functional Perspective

    DEFF Research Database (Denmark)

    Tarp, Sven

    2014-01-01

    This contribution provides an overview of some of the methods used in relation to the function theory. It starts with a definition of the concept of method and the relation existing between theory and method. It establishes an initial distinction between artisanal and theory-based methods...

  19. Answer Card Identification Method Based on Pattern Recognition Technology

    OpenAIRE

    Xu Jing; Zhang Kun Fang

    2016-01-01

    The traditional answer card reading method using OMR (Optical Mark Reader), most commonly, OMR special card special use, less versatile, high cost, aiming at the existing problems proposed a method based on pattern recognition of the answer card identification method. Using the method based on Line Segment Detector to detect the tilt of the image, the existence of tilt image rotation correction, and eventually achieve positioning and detection of answers to the answer sheet .Pattern recogniti...

  20. Pressure on Cultivated Land in Fengxian County,Jiangsu Province,China

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Based on the introduction of the general situation of research region,pressure index model of cultivated land is adopted according to the data of population,cultivated land area,crop sowing area,grain sowing area,and unit grain yield in the Statistical Yearbook of Fengxian County.The change of relevant factors of cultivated land pressure index over time is analyzed,as well as the tension level of cultivated land resources.Research shows that the pressure on cultivated land is relatively great in Fengxian County in the years 1999-2005.Grain supply and demand is still under the unsafe state.There will be more prominent contradiction between supply and demand of cultivated land in Fengxian County in the future,and the grain security will under greater pressure.Several countermeasures are put forward to ease the pressure on cultivated land in Fengxian County,in order to realize the sustainable use of cultivated land resources,to ensure the supply and demand balance of grain and cultivated land,and to stabilize and improve the grain productivity in Fengxian County,such as protecting the current cultivated land resources by the strictest protection system of cultivated land,enhancing the intensity of land development and reclamation,enlarging the area of cultivated land,increasing the agricultural sci-tech input,and improving the grain unit yield.

  1. AN IMAGE RETRIEVAL METHOD BASED ON SPATIAL DISTRIBUTION OF COLOR

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Color histogram is now widely used in image retrieval. Color histogram-based image retrieval methods are simple and efficient but without considering the spatial distribution information of the color. To overcome the shortcoming of conventional color histogram-based image retrieval methods, an image retrieval method based on Radon Transform (RT) is proposed. In order to reduce the computational complexity,wavelet decomposition is used to compress image data. Firstly, images are decomposed by Mallat algorithm.The low-frequency components are then projected by RT to generate the spatial color feature. Finally the moment feature matrices which are saved along with original images are obtained. Experimental results show that the RT based retrieval is more accurate and efficient than traditional color histogram-based method in case that there are obvious objects in images. Further more, RT based retrieval runs significantly faster than the traditional color histogram methods.

  2. 毛囊细胞高效分离培养方法的建立%Establishment of High Performance Method for Isolation and Cultivation of Hair Follicle Stem Cells of Neonatal Rats

    Institute of Scientific and Technical Information of China (English)

    陈杏晔; 李凡; 刘爱军

    2014-01-01

    Objective To establish a simple , practical , highly-effective and stable method for the isolation and cultivation of rat hair follicle cells. Methods Under sterile condition, single hair follicle was taken out after the skin around the barbel of SD neonatal rats was sheared off. And then the hair follicles were digested with two-step method with Type Ⅰcollagenase and trypsin. The obtained cell suspension was planted into the culture plate which was covered with extracellular matrix, and then was cultivated with K-SFM culture medium containing fetal bovine serum with volume fraction of 1%. On the next day, K-SFM culture medium was replaced with serum-free culture medium. The remaining tissues were cut into pieces and spread out in the culture flask, and then were cultivated with HG-DMEM culture medium containing serum. Two kinds of cells were harvested and then were identified by immunofluorescence. The hair follicle epithelial cells were tested by flow cytometer. Results The hair follicle epithelial cells obtained through the above methods showed rapid adherence, and were round or polygon-like , with typical cobblestone-like morphology. The long spindle-shaped cells were seen around the tissues cultivated, having many protrusions on the surface of the cells, and they were interconnected into reticular structure. The expression of cytokeratin 15, cytokeratin 19 and β1 integrin in epithelial cells were positive. Most of the epithelial cells were in the G1 phase, accounting for 75.6%. The expression of laminin ( LN), fibronectin ( FN) and vimentin in the connective tissue sheath cells were also positive. Conclusion The cells harvested by modified two-step enzyme digestion method have confirmed as hair follicle cells and fibroblasts, and the obtained cells are of rapid adherence, good homogeneity, and active proliferation.%【目的】建立一种简单易行、高效稳定、细胞活性好的毛囊细胞分离培养方法。【方法】无菌条件下剪下SD乳

  3. The use of 32P Method to Evaluate the Growth of Lowland Rice Cultivated in a System of Rice Intensification (SRI

    Directory of Open Access Journals (Sweden)

    A. Citraresmini

    2013-08-01

    Full Text Available A pot experiment has been conducted to evaluate the growth of the Dyah Suci, a lowland rice variety, in an SRI (System of Rice Intensification planting system. The phosphorus-32 (32P isotope technique was used to evaluate the growth of plants in relation with their phosphorus uptake. The uptake was assumed to vary in the same direction as the growth of the plant. The 32P uptake is assumed to vary in the opposite direction to the plant’s total phosphorus uptake. Here the 32P uptake is expressed in count per minutes (cpm which is then transformed to disintegration per minute (dpm. The results show that, in terms of promoting the plant’s uptake of phosphorus, the SRI planting system is superior to the conventional planting system, and it is manifested in the higher dry weight of straw and grain. From this experiment it is concluded that the 32P method can be used satisfactorily as a tool for explaining the relation between P-uptake and plant growth

  4. 基于职业能力培养的市场营销课程教学改革%Research on Teaching Reform in Marketing Course Based on Vocational Competency Cultivation

    Institute of Scientific and Technical Information of China (English)

    崔玉华

    2012-01-01

    职业能力培养是市场营销课程适应市场、服务企业、创新人才培养模式的需要。从市场营销课程内容的改革、教学方法的改革、考试的改革以及教学中应当把握的原则4个方面,提出了市场营销课程改革必须从知识传授型向职业技能型转变,从课堂为中心向社会实践为中心转变,变教师为教练,变封闭学习为开放学习的建议。%Vocational competency cultivation is the needs of marketing course adapting to the market, serving enter-prises, and innovating talent cultivation model. This paper proposes that teaching reform in marketing course should be from knowledge initiating to vocational skills, from classroom - centered to practice - centered, from teachers to coaches, from closed -end learning to open learning through the research on the reform of course content, the re- form of teaching method, the reform of examination and the teaching principles.

  5. Study on cultivation objectives and curriculum system of nursing postgraduate constructed by Delphi method%Delphi法构建护理学研究生培养目标及课程体系的研究

    Institute of Scientific and Technical Information of China (English)

    蒋小平; 颜莉; 郑显兰; 刘贤; 魏晓琼

    2015-01-01

    Objective:To construct the specific ,detailed and operable cultivation objectives of nursing master postgraduates and curriculum system in favor of the cultivation of core competency of nursing professional mas‐ter ,so as to provide the references for the training program of medical colleges and higher nursing education management .Methods:By investigating the related literatures of training scheme of nursing academic type mas‐ter postgraduates in Chinese and English database various colleges and universities public web sites in nearly 5 years in 31 domestic and foreign universities ,and combining with Taylor’s education target model of theory frame ,Delphi specialist letter questionnaire was drafted ,a total of 2 rounds of inquiry were carried out .Statisti‐cal analysis was performed for the positive coefficient ,authoritative coefficient of expert ,expert opinion coordi‐nation degree and the mean value of indexes at all levels ,and coefficient of variation ,and to analyze the practica‐bility and significance of all indexes .Results:A total of 26 experts participated in inquiry ,value of their authority degree was 0 .88 ,and Kendall coordinated coefficient was 0 .531 ,χ2 test showed P<0 .01 ;the cultivation objec‐tives formed through two rounds of expert consultation had 9 concrete items ,including knowledge ,skills and humanistic accomplishment ;the formed curriculum system included 30 subjects totally ,in which there were 4 public obligatory courses ,6 professional basic courses ,20 professional expand option courses .Conclusion:The construction of nursing postgraduate cultivation objectives and curriculum system formed in this study were more specific and operable ,can provide references for development of postgraduate cultivation scheme of nursing profession .%[目的]构建具体、细化及可操作的护理学硕士研究生培养目标和有利于护理专业硕士生核心能力培养的课程体系,为各医学院校和高等护理教

  6. Mass Cultivation of Entomopathogenic Nematode in Artificial Media

    OpenAIRE

    Dyah Rini Indriyanti; Nur Lailatul Muharromah

    2016-01-01

    Entomopathogenic nematodes Entomopathogenic nematodes (EPN) of the genera Heterorhabditis and Steinernema are commercially used to control pest insect. EPN is widely cultivated through in-vivo and in vitro methods. This research aims to discover the abundance of EPN cultivated in various artificial media. Seven types of media composition were tested in this research: media A (yeast + soybean powder), media B (yeast + chicken liver), media C (yeast + dog food), media D (yolk + soybean powder),...

  7. A Study and Practice of College Students' Emotional Quotient Cultivation Based on Quality Development%基于素质拓展平台大学生情商培养研究与实践

    Institute of Scientific and Technical Information of China (English)

    嵇敏; 杨丹; 李冠华; 张玲彬

    2015-01-01

    基于素质拓展平台新模式运作下大学生情商培养研究与实践,通过在河海大学大禹学院2013级本科生中开展野外素质拓展,寒暑假感恩教育,社会实践、公益劳动等素质拓展学分的系列活动,在实践的强化培养下,增强学生的团结、爱心、宽容、感恩、自信等情商特性,以促进学生更好地认识自我、管理自我、激励自我。%This paper is a study and practice of college students' Emotional Quotient cultivation based on a new model which is quality development. In Grade 2013 undergraduates in Dayu College of Hohai University, field quality development, thanks-giving education during summer and winter vacation, social prac-tice, welfare work and other activities of quality development credits were held. Under the practice of intensive cultivation, students' non-intelligence characteristics of EQ, such as solidar-ity, love, tolerance, thanksgiving and self-confidence, , were strengthened, and they could understand, manage themselves, and motivate themselves better.

  8. 基于应用型人才培养的HRM本科专业课程体系设计%Design of HRM Undergraduate Curriculum System Based on Cultivating Practical Talents

    Institute of Scientific and Technical Information of China (English)

    徐进

    2013-01-01

    Training objectives and specifications of specialty finally needs to be realized through the perfect curriculum system. Curriculum system design is the core content of the specialized training plan, it is also the con-crete externalization of features and highlights of specialty. HRM is a high practical specialty, for realizing the ob-jectives of cultivating practical talent, how to construct HRM undergraduate curriculum system based on cultivat-ing practical talents is a realistic and important issue.%专业培养目标和培养规格最终要通过完善的课程体系来实现。课程体系设计是专业培养方案最核心的内容,也是专业特色和亮点的具体体现。HRM(人力资源管理)本科专业是一门实践性很强的专业。应用型培养目标下,如何构建基于应用型人才培养的HRM本科专业课程体系是一个现实而重要的课题。

  9. 基于创新能力培养的市场营销学课程教学改革探索%Marketing Course Teaching Reform Based on the Cultivation of Innovation Ability

    Institute of Scientific and Technical Information of China (English)

    付存军

    2012-01-01

      The nature of the marketing demands innovation,the drastic changes in the social and economic environment requires that Marketers must innovatively apply the marketing theory to solve the new problems emerging ceaselessly, so the marketing course teaching should focus on the cultivation of students' innovative ability. To achieve this goal, first, the cultivation of students' innovative ability should be embodied in the curriculum teaching target planning, secondly, the marketing curriculum content should properly adjusted, thirdly, it should be flexibly applied in the teaching process of questioning teaching method, scene teaching method, case teaching method and practice teaching method, finally it should be reflected the cultivation of students' innovative ability in course evaluation%  市场营销学的本质要求创新,急剧变化的社会经济环境要求市场营销者必须具备创新性运用市场营销理论解决不断出现的新问题的能力,因而市场营销学课程教学应注重对学生创新能力的培养。为实现该目标,首先应将学生创新能力的培养要求体现在课程教学培养目标规划中,其次应对市场营销学课程内容进行恰当的调整,再次应该在课程教学过程中灵活运用设疑式教学法、情景式教学法、案例教学法及实践教学法等方式方法,最后在课程考核中应该体现出对学生创新能力培养的重视。

  10. Systems and methods for interpolation-based dynamic programming

    KAUST Repository

    Rockwood, Alyn

    2013-01-03

    Embodiments of systems and methods for interpolation-based dynamic programming. In one embodiment, the method includes receiving an object function and a set of constraints associated with the objective function. The method may also include identifying a solution on the objective function corresponding to intersections of the constraints. Additionally, the method may include generating an interpolated surface that is in constant contact with the solution. The method may also include generating a vector field in response to the interpolated surface.

  11. High-cell-density cultivation of microorganisms.

    Science.gov (United States)

    Riesenberg, D; Guthke, R

    1999-04-01

    High-cell-density cultivation (HCDC) is required to improve microbial biomass and product formation substantially. An overview of HCDC is given for microorganisms including bacteria, archae and eukarya (yeasts). Problems encountered by HCDC and their possible solutions are discussed. Improvements of strains, different types of bioreactors and cultivation strategies for successful HCDC are described. Stirred-tank reactors with and without cell retention, a dialysis-membrane reactor, a gas-lift reactor and a membrane cyclone reactor used for HCDC are outlined. Recently modified traditional feeding strategies and new ones are included, in particular those for unlimited growth to very dense cultures. Emphasis is placed on robust fermentation control because of the growing industrial interest in this field. Therefore, developments in the application of multivariate statistical control, artificial neural networks, fuzzy control and knowledge-based supervision (expert systems) are summarized. Recent advances using Escherichia coli--the pioneer organism for HCDC--are outlined. PMID:10341426

  12. Evaluation on Cultivated Land Resource Ecologi-cal Service Value in Wuhan Metropolitan Area

    Institute of Scientific and Technical Information of China (English)

    Jinhua TANG; Yinghui Ll; Gangqiang CHEN; Lei YU; Sujuan Ll

    2014-01-01

    The land ecosystem service function value contains the gas regulating function value, air purification function value, water conservation value, land conser-vation value, soil nutrient conservation value and maintain biodiversity value. Based on the method of market value and the data of Hubei Statistical yearbook, cultivat-ed land resources of ecosystem service function value is 730 038.69Mil ion in 2007-2009, and 714 774.99 Mil ion in 2010-2012 in Wuhan Metropolitan Area. After three years of development, Wuhan Metropolitan Area of ecological value of cultivated land has increased by about 3.97%. From the point of each composition, the im-portance of gas regulating function of cultivated land resources and water conserva-tion function is the forefront. From the point of spatial distribution, the highest of annual average value of the cultivated land ecological was Huanggang (1.725 bil ion yuan), the lowest was Ezhou (217.868 6 mil ion yuan) in 2010-2012. From the point of space changes, the land ecological value increased only in Qianjiang city, while farmland ecological value in other cities was fal ing The conclusion is of direc-tive significance to the regional land protection policy and the farmland reasonable distribution, for Wuhan Metropolitan Area and other areas.

  13. Antimicrobial activity and mineral composition of shiitake mushrooms cultivated on agricultural waste

    Directory of Open Access Journals (Sweden)

    Kérley Braga Pereira Bento Casaril

    2011-10-01

    Full Text Available The antimicrobial activity and mineral composition of shiitake mushrooms were evaluated in four isolates of Lentinula edodes. Mushrooms were cultivated on artificial logs, based on eucalyptus sawdust enriched with 20% rice, wheat, or soybean bran, or combination of 10% of two of these supplements. The substrates were humidified with a 0.1% mate tea extract or water. Logs of Eucalyptus grandis were also used to cultivate the shiitake mushrooms. The antimicrobial activity of an aqueous extract, corresponding to 40 mg of mushroom dry matter, was in some cases, depending on the isolate, able to inhibit both Bacillus subtilis and Escherichia coli K-12, independent of substrate composition or the growth stage of the mushrooms. Nitrogen, phosphorus, potassium, magnesium and calcium concentrations varied according to the substrate on which the mushrooms were cultivated, being, generally, higher with cultivation on artificial rather than natural eucalyptus logs. It could be concluded that, in addition to the fungal isolate, substrate composition and, processing methods must be considered during the production of antimicrobial substance(s as well as in the mushroom nutritional composition.

  14. Utilization of industrial dairy waste as microalgae cultivation medium : a potential study for sustainable energy resources

    Science.gov (United States)

    Nurmayani, S.; Sugiarti, Y.; Putra, R. H.

    2016-04-01

    Microalgae is one of biodiesel resources and call as third generation biofuel. Biodiesel is one alternative energy that being developed. So study about resource of biodiesel need a development, for the example is development the basic material such as microalgae. In this paper we explain the potential use of dairy waste from industry as a cultivation medium of microalgae for biodiesel production. Dairy waste from dairy industry contains 34.98% protein, 4.42% lactose, 9.77% fiber, 11.04% fat, 2.33% calcium, 1.05% phosfor, and 0.4 % magnesium, meaning that the dairy waste from dairy industry has a relatively high nutrient content and complete from a source of carbon, nitrogen and phosphorus as macro nutrients. The method in this paper is literature review to resulting a new conclusion about the potency of waste water from dairy industry as microalgae cultivation medium. Based on the study, the dairy waste from dairy industry has potency to be used as cultivation medium of Botryococcus braunii in the production of biodiesel, replacing the conventional cultivation medium.

  15. A study on Heavy Metal Contents in Cultivated Wild Ginseng from Korea and China

    Directory of Open Access Journals (Sweden)

    Jong-Yoon,Kim

    2007-02-01

    Full Text Available Objectives : Heavy metal contents in cultivated wild ginseng from Korea and China were evaluated for the safety purpose. Methods : Arsenic(As, cadmium(Cd, lead(Pb, mercury(Hg, copper(Cu, chromium(Cr, and selenium(Se from the root, stem, and leaves of the Korean cultivated wild ginseng and the root of the Chinese cultivated wild ginseng were analyzed for the existence of heavy metal contents. Results : Most of heavy metals weren't detected in all the samples. Copper and lead were found in very minute volume and didn't reach the toxic concentration level of 10mg/kg. Samples used in the test were grown in non-contaminated areas, free from heavy metal contamination. Conclusions : Based on above results, cultivated wild ginseng from Korea and China are relatively safe from the heavy metal exposure. But for more rigorous quality control, an epidemiological survey using the samples from more diverse areas should be conducted.

  16. Effects of Urbanization-Induced Cultivated Land Loss on Ecosystem Services in the North China Plain

    Directory of Open Access Journals (Sweden)

    Wei Song

    2015-06-01

    Full Text Available Since the implementation of market oriented economic reform in 1978, China has been on the track of rapid urbanization. The unprecedented urbanization in China has resulted in substantial cultivated land loss and rapid expansion of urban areas. The cultivated land loss due to urbanization not only threatens food security in China, but has also led to ecological system degradation to which close attention should be paid. Therefore, we examined the effects of the conversion from cultivated to urban areas on the ecosystem service in the North China Plain on the basis of a net primary productivity based ecosystem service model (NESM and a buffer comparison method. Cultivated land loss due to urbanization in the North China Plain led to a total loss of ecosystem service value of 34.66% during the period 1988–2008. Urban expansion significantly decreased the ecosystem service function of water conservation (–124.03%, nutrient cycling (–31.91%, gas regulation (−7.18%, and organic production (–7.18%, while it improved the soil conservation function (2.40%. Land use change accounted for 57.40% of the changes in ecosystem service and had a major influence on the changes in nutrient cycling and water conservation. However, climate change mainly determined the changes in gas regulation, organic production, and soil conservation.

  17. Three Strategies for Elaborating the Cultivation Hypothesis.

    Science.gov (United States)

    Potter, W. James

    1988-01-01

    Uses three strategies (dividing cultivation into component subprocesses, testing for an intervening variable, and contingent relationships) for elaborating the cultivation hypothesis. Finds evidence that cultivation effects do exist but that dividing the socialization process does not increase the predictive power of the cultivation hypothesis.…

  18. 基于个性心理的婴幼儿独立意识培养%Independent Awareness Cultivation of Infants Based on Individual Psychology

    Institute of Scientific and Technical Information of China (English)

    关金艳

    2013-01-01

      独立意识是评价人体心理健康成熟与否的重要标准。在婴幼儿教育领域,独立意识是婴幼儿心理健康成熟和心理健康发展的重要指标。通过独立意识的培养能够促进婴幼儿运动能力、语言能力、思维能力、智力,以及社会适应能力的全面发展。目前国内外学者对婴幼儿独立意识的研究主要是从年龄、生理过程、独立性特性,以及教学方面进行的,缺乏从心理学专业角度分析其成因、干预对策和效果。为培养婴幼儿的独立意识,首先要营造常态化的和谐的家庭氛围,其次要打造稳定的心理教学队伍,再次要通过优秀的动画片等向婴幼儿传递独立意识。%Independent awareness is an important standard to evaluate whether a person is mature in mental mealth .In the field of infant education ,independent awareness is an important index of the mental maturi‐ty and mental health development of the infants .The cultivation of independent awareness can promote the all‐round development of infants’ ability to do sports ,to speak ,to think and their intelligence and their social adaptability .At present foreign scholars study the independent awareness of infants mainly from such aspects as age ,physiological process ,independence adaptability and teaching ,which lacks profession‐al analysis of its causes ,intervention countermeasures and effects from psychological perspective .In order to cultivate the infants’ independent consciousness ,we must create a normal and harmonious family at‐mosphere in the first place .Secondly ,we should foster stable teaching staff of psychology .Finally we should teach the infants independent awareness through excellent cartoons .

  19. Independent Awareness Cultivation of Infants Based on Individual Psychology%基于个性心理的婴幼儿独立意识培养

    Institute of Scientific and Technical Information of China (English)

    关金艳

    2013-01-01

      独立意识是评价人体心理健康成熟与否的重要标准。在婴幼儿教育领域,独立意识是婴幼儿心理健康成熟和心理健康发展的重要指标。通过独立意识的培养能够促进婴幼儿运动能力、语言能力、思维能力、智力,以及社会适应能力的全面发展。目前国内外学者对婴幼儿独立意识的研究主要是从年龄、生理过程、独立性特性,以及教学方面进行的,缺乏从心理学专业角度分析其成因、干预对策和效果。为培养婴幼儿的独立意识,首先要营造常态化的和谐的家庭氛围,其次要打造稳定的心理教学队伍,再次要通过优秀的动画片等向婴幼儿传递独立意识。%Independent awareness is an important standard to evaluate whether a person is mature in mental mealth .In the field of infant education ,independent awareness is an important index of the mental maturi‐ty and mental health development of the infants .The cultivation of independent awareness can promote the all‐round development of infants’ ability to do sports ,to speak ,to think and their intelligence and their social adaptability .At present foreign scholars study the independent awareness of infants mainly from such aspects as age ,physiological process ,independence adaptability and teaching ,which lacks profession‐al analysis of its causes ,intervention countermeasures and effects from psychological perspective .In order to cultivate the infants’ independent consciousness ,we must create a normal and harmonious family at‐mosphere in the first place .Secondly ,we should foster stable teaching staff of psychology .Finally we should teach the infants independent awareness through excellent cartoons .

  20. Environmental life cycle assessment of Ethiopian rose cultivation.

    Science.gov (United States)

    Sahle, Abiy; Potting, José

    2013-01-15

    A life cycle assessment (LCA) was conducted for Ethiopian rose cultivation. The LCA covered the cradle-to-gate production of all inputs to Ethiopian rose cultivation up to, and including transport to the Ethiopian airport. Primary data were collected about materials and resources used as inputs to, and about the product outputs from 21 farms in 4 geographical regions (i.e. Holleta, Sebeta, Debre Ziet, and Ziway). The primary data were imported in, and analyzed with the SimaPro7.3 software. Data for the production of used inputs were taken from the EcoInvent®2.0 database. Emissions from input use on the farms were quantified based on estimates and emission factors from various studies and guidelines. The resulting life cycle inventory (LCI) table was next evaluated with the CML 2 baseline 2000 V2/world, 1990/characterization method to quantify the contribution of the rose cultivation chain to 10 environmental impact categories. The set of collected primary data was comprehensive and of high quality. The data point to an intensive use of fertilizers, pesticides, and greenhouse plastic. Production and use of these inputs also represent the major contributors in all environmental impact categories. The largest contribution comes from the production of the used fertilizers, specifically nitrogen-based fertilizers. The use of calcium nitrate dominates Abiotic Depletion (AD), Global Warming (GW), Human Toxicity (HT) and Marine Aquatic Ecotoxicity (MAET). It also makes a large contribution to Ozone Depletion (OD), Acidification (AD) and Fresh water Aquatic Ecotoxicity (FAET). Acidification (AC) and Eutrophication (EU) are dominated by the emission of fertilizers. The emissions from the use of pesticides, especially insecticides dominate Terrestrial Ecotoxicity (TE) and make a considerable contribution to Freshwater Aquatic Ecotoxicity (FAET) and Photochemical Oxidation (PhO). There is no visible contribution from the use of pesticides to the other toxicity categories

  1. Utilization zoning of cultivated land based on net primary productivity in Guanzhong-Tianshui Economic Region%基于净初级生产力的关中-天水经济区耕地利用分区研究

    Institute of Scientific and Technical Information of China (English)

    杨海娟; 温晓金; 刘焱序

    2013-01-01

    As a populous nation, improve grain production capacity along with rational use and protection of cultivated land resources has posed a considerable challenge in domestic agriculture and land related research in China. Higher NPP for cultivated lands has suggested the existence of more organic biomass. This has been critical for the final production of food crops in the country. It was therefore likely for research on NPP to provide the basis for resolving food security issues. Functional zoning has been the commonly used method to guarantee sustainable use of land. Presently, however, heavily fragmented research merely described real supply of cultivated lands. A deeper understand on the potential reserves of cultivated lands was needed in this regard. Based on remote sensing observation, it is possible to have statistics of the output of a large number of cultivated lands within a short time. Compared with the yearbook data, remote sensing observation has advantages including timeliness and spatial precision. Remote sensing observations have therefore been strongly supplemental to statistical data. NPP estimated by remote sensing was used as crop biomass in cultivated lands instead of the traditional calculations based statistics data. Cultivated land in the Guanzhong-Tianshui Economic Region (GTER) was zoned by using neural network algorithm model and remote sensing data in 2001-2009 substituting for statistic crop yield data. Then the wavelet neural network was used to predict the NPP in the zoned regions. Three results were eventually attained. 1) From 2002 to 2009, total estimated NPP per year in GTER was 1.6×107 t. It showed large variation patterns between estimated NPP data and statistics grain data for cultivated lands in GTER. This suggested statistical and remote sensing data were not substitutable for one another. As clustering function was unknown, zoning via estimated NPP data reflected a more universal adaptability than via statistical data. 2) The

  2. Method for detecting software anomalies based on recurrence plot analysis

    Directory of Open Access Journals (Sweden)

    Michał Mosdorf

    2012-03-01

    Full Text Available Presented paper evaluates method for detecting software anomalies based on recurrence plot analysis of trace log generated by software execution. Described method for detecting software anomalies is based on windowed recurrence quantification analysis for selected measures (e.g. Recurrence rate - RR or Determinism - DET. Initial results show that proposed method is useful in detecting silent software anomalies that do not result in typical crashes (e.g. exceptions.

  3. Correlation theory-based signal processing method for CMF signals

    Science.gov (United States)

    Shen, Yan-lin; Tu, Ya-qing

    2016-06-01

    Signal processing precision of Coriolis mass flowmeter (CMF) signals affects measurement accuracy of Coriolis mass flowmeters directly. To improve the measurement accuracy of CMFs, a correlation theory-based signal processing method for CMF signals is proposed, which is comprised of the correlation theory-based frequency estimation method and phase difference estimation method. Theoretical analysis shows that the proposed method eliminates the effect of non-integral period sampling signals on frequency and phase difference estimation. The results of simulations and field experiments demonstrate that the proposed method improves the anti-interference performance of frequency and phase difference estimation and has better estimation performance than the adaptive notch filter, discrete Fourier transform and autocorrelation methods in terms of frequency estimation and the data extension-based correlation, Hilbert transform, quadrature delay estimator and discrete Fourier transform methods in terms of phase difference estimation, which contributes to improving the measurement accuracy of Coriolis mass flowmeters.

  4. Teaching based on the case method of building collective protection

    OpenAIRE

    Gonzalez Garcia, Nieves; Cobo Escamilla, Alfonso; Moreno Fernandez, Maria Esther

    2013-01-01

    One of the fundamental aspects in the adaptation of the teaching to the European higher education is changing based models of teacher education to models based on student learning. In this work we present an educational experience developed with the teaching method based on the case method, with a clearly multidisciplinary. The experience has been developed in the teaching of analysis and verification of safety rails. This is a multidisciplinary field that presents great difficulties during t...

  5. Qualitative Comparison of Contraction-Based Curve Skeletonization Methods

    NARCIS (Netherlands)

    Sobiecki, André; Yasan, Haluk C.; Jalba, Andrei C.; Telea, Alexandru C.

    2013-01-01

    In recent years, many new methods have been proposed for extracting curve skeletons of 3D shapes, using a mesh-contraction principle. However, it is still unclear how these methods perform with respect to each other, and with respect to earlier voxel-based skeletonization methods, from the viewpoint

  6. Classification-Based Method of Linear Multicriteria Optimization

    OpenAIRE

    Vassilev, Vassil; Genova, Krassimira; Vassileva, Mariyana; Narula, Subhash

    2003-01-01

    The paper describes a classification-based learning-oriented interactive method for solving linear multicriteria optimization problems. The method allows the decision makers describe their preferences with greater flexibility, accuracy and reliability. The method is realized in an experimental software system supporting the solution of multicriteria optimization problems.

  7. 基于形象思维的创新能力培养的分析%Analysis of Cultivation for Innovation Ability Based on Thinking in Images

    Institute of Scientific and Technical Information of China (English)

    赵晓运

    2012-01-01

    分析了创新教育中形象思维的独特性、基础性和源头性,指出创新能力是一种社会属性,可以通过锻炼和培养而得到提高.首次提出了结构功能原理“使用情景”的形象认识.通过对教学实践和创新应用实践的分析,强调了训练和触发形象思维能力,可以有效地、显著地提高创新思维能力.%Uniqueness, foundation and fountainhead of thinking in images in innovation education are analyzed. Innovation ability is one type of social attributes and can be improved through practice and cultivation. Imaginal recognition of usage scenario to structural function principles is initially proposed. Practice and triggering of imaginal thinking ability, which can effectively and markedly enhance innovation thinking ability, are emphasized under the analysis of teaching practices and innovation applied practices.

  8. 基于P BLL的大学生英语自主式学习能力培养研究%Cultivating University Students’ English Autonomous Learning Ability Based on PBLL

    Institute of Scientific and Technical Information of China (English)

    李明秋; 张光耀; 孙欣; 石小妹; 武瑶伊

    2015-01-01

    PBLL( Project-Based Language Learning, short for PBLL) is a kind of learner-oriented learning mode,also known as“Zhuantishi Research” or“Project Learning”. Based on PBLL mode,it needs all aspects of university’ s administration,administer,and students themselves work together and work respectively to cultivate the students’ autonomous learning ability,including carrying out the division of the relevant departments to culti-vate the autonomous learning ability;building English autonomous learning platform;establishing the autonomous learning model in the classroom learning environment;increasing the responsibility of autonomous learning from the perspective of teachers;improving the students’ autonomous learning ability and establishing the new autono-mous learning mode in information network.%依托项目的语言学习( Project-Based Language Learning,简称PBLL)是一种以学习者为中心的学习模式,又称为“项目式学习”“专题式研究”。依据PBLL模式,高校的各个职能部门、教学单位,管理者、教师和学习者既需各司其职又要通力合作来培养大学生的自主学习能力,包括落实形成英语自主学习能力的各自分工任务;构建英语自主学习平台;建立课堂环境的自主学习模式;明确英语自主学习能力培养过程中的教师职责;提升学生自主学习能力素质和创建信息化网络自主学习新模式。

  9. Bionic optimization research of soil cultivating component design

    Institute of Scientific and Technical Information of China (English)

    GUO ZhiJun; ZHOU ZhiLi; ZHANG Yi; LI ZhongLi

    2009-01-01

    The basic biomechanical laws that apply to the clawed toes of animals with powerful digging abilities and the optimal bionic design of curved soil cultivating components with an analogous contour were researched in a novel way. First, the curvature and profile of the inside contour line of a field mouse's clawed toe were analyzed. The finite element method (FEM) was then used to simulate the working process in order to study the changing characteristics of the working resistance of bionic soil-engaging surfaces and the stress field of the processed soil. A straight-line cultivating component was used for comparative analysis. In accordance with the simulation results, a series of soil cultivating components of varying design were manufactured. An indoor soil bin experiment was carried out to measure their working resistance and validate the results of the FEM analysis. The results of this research would have important values in the optimization design of cultivating components for energy and cost savings.

  10. A facile method for the preparation of chitosan-based scaffolds with anisotropic pores for tissue engineering applications.

    Science.gov (United States)

    Ran, Jiabing; Xie, Lingjun; Sun, Guanglin; Hu, Jingxiao; Chen, Si; Jiang, Pei; Shen, Xinyu; Tong, Hua

    2016-11-01

    To date, great efforts have been made to prepare different kinds of isotropic tissue engineering (TE) scaffolds. However, little attention has been paid to anisotropic porous scaffolds in spite of many examples of their excellent performances. In this work, a facile method termed "ammonia-induced method" (AIM) was proposed and applied to generate anisotropic pores in chitosan (CS)-based scaffolds. The pore structures of these scaffolds were studied in detail. In order to clarify the rationale behind this process, a speculative explanation was provided on basis of the experimental results and the theory of Uras (Uras & Devlin, 2000). Compression tests indicated that the mechanical strengths of these scaffolds were sufficient for TE applications. In vitro cell culture showed that MC3T3-E1 cells cultivated in the pores of these scaffolds had positive proliferation potential. We anticipated that this novel AIM could inspire research not only in TE but also in other fields. PMID:27516311

  11. Nitrous oxide emissions from rapeseed cultivation in Germany

    Science.gov (United States)

    Fuß, Roland; Andres, Monique; Hegewald, Hannes; Kesenheimer, Katharina; Koebke, Sarah; Räbiger, Thomas; Suárez Quiñones, Teresa; Walter, Katja; Stichnothe, Heinz; Flessa, Heinz

    2016-04-01

    About 12 % of Germany's agricultural area is used for rapeseed cultivation and two third of the harvest is converted to biodiesel. Due to requirements of the EU Renewables Directive the greenhouse gas (GHG) balance of rapeseed cultivation must be reported and sustainability criteria and GHG savings compared to fossil fuel must be achieved and certified. Current certified methodology estimates N2O field emissions from rapeseed cultivation using the IPCC Tier 1 approach based on a global emission factor (N2O emission per unit nitrogen fertilizer input) of 1 %, which is not specific for the crop. We present results from three years of measurements (2013 - 2015) on five field trials in Germany, which combined with data from a meta-analysis suggest that GHG emission factors of German rapeseed cultivation are lower than thought previously. Furthermore, results suggest that substitution of mineral fertilizers with organic fertilizers is a valid mitigation option since it avoids GHG emissions during production of mineral fertilizers.

  12. Statistical Analysis of the Cultivating Methods of Construction Workers' Safe Behavior Habits%建筑工人安全行为习惯培养方法的统计分析

    Institute of Scientific and Technical Information of China (English)

    杨高升; 居婕

    2013-01-01

    建筑工人的不安全行为直接导致了安全事故的发生,从行为心理学的角度分析了建筑工人不安全行为形成机理,并从中找出影响因素.细分安全行为习惯的培养方法:正强化、负强化、惩罚、教育培训,并通过对南京市两家建筑单位的管理人员进行问卷抽样调查,利用SPSS软件对安全行为习惯培养的四种方法进行统计分析,其中安全教育培训最为重要,结合国外安全培训模式经验,提出可行性的措施.%The construction workers unsafe behavior is the direct cause of security incidents. Analyzes the formation mechanism of construction workers' unsafe behavior from the perspective of behavior psychology, and finds out the factors. Subdivides the cultivating methods of safe behavior habits into four sectors, namely, positive reinforcement, negative reinforcement, punishment, and training. Through the questionnaire survey on the management of the two building units in Nanjing and using the SPSS software to conduct the statistical analysis of the four methods of fostering safe behavior habits, this paper finds that the safe education and training are the most important factors to foster safe behavior habits. At last, this paper gives the feasible measures combined with foreign experience in safety training mode.

  13. Highly sensitive methods for electroanalytical chemistry based on nanotubule membranes.

    Science.gov (United States)

    Kobayashi, Y; Martin, C R

    1999-09-01

    Two new methods of electroanalysis are described. These methods are based on membranes containing monodisperse Au nanotubules with inside diameters approaching molecular dimensions. In one method, the analyte species is detected by measuring the change in trans-membrane current when the analyte is added to the nanotubule-based cell. The second method entails the use of a concentration cell based on the nanotubule membrane. In this case, the change in membrane potential is used to detect the analyte. Detection limits as low as 10(-11) M have been achieved. Hence, these methods compete with even the most sensitive of modern analytical methodologies. In addition, excellent molecular-sized-based selectivity is observed.

  14. Conceptual bases of the brand valuation by cost method

    Directory of Open Access Journals (Sweden)

    G.Y. Studinska

    2015-03-01

    Full Text Available The necessity of valuing intangible assets in accordance with international trends is substantiated. The brand is seen as more important component of intangible assets, as an effective management tool company. The benefits and uses of brand evaluation results are investigated. System monocriterion cost brand evaluation methods is analyzed. In particular, methods that require evaluation by the time factor (current and forecast methods and methods for factor comparison base (relative and absolute. The cost method of brand valuation through market transactions in accordance J.Common’s classification is considered in detail. The explanation of the difference between method a summation of all costs and method of brand valuation through market transactions is provided. The advantages and disadvantages considered cost method of brand valuation are investigated. The cost method as the relative-predicted of the brand valuation, «The method of determining the proportion of the brand from the discounted total costs» is grounded

  15. A Spatialization-based Method for Checking and Updating Metadata

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    In this paper the application of spatialization technology on metadata quality check and updating was discussed. A new method based on spatialization was proposed for checking and updating metadata to overcome the deficiency of text based methods with the powerful functions of spatial query and analysis provided by GIS software. This method employs the technology of spatialization to transform metadata into a coordinate space and the functions of spatial analysis in GIS to check and update spatial metadata in a visual environment. The basic principle and technical flow of this method were explained in detail, and an example of implementation using ArcMap of GIS software was illustrated with a metadata set of digital raster maps. The result shows the new method with the support of interaction of graph and text is much more intuitive and convenient than the ordinary text based method, and can fully utilize the functions of GIS spatial query and analysis with more accuracy and efficiency.

  16. A New Adaptive Image Denoising Method Based on Neighboring Coefficients

    Science.gov (United States)

    Biswas, Mantosh; Om, Hari

    2016-03-01

    Many good techniques have been discussed for image denoising that include NeighShrink, improved adaptive wavelet denoising method based on neighboring coefficients (IAWDMBNC), improved wavelet shrinkage technique for image denoising (IWST), local adaptive wiener filter (LAWF), wavelet packet thresholding using median and wiener filters (WPTMWF), adaptive image denoising method based on thresholding (AIDMT). These techniques are based on local statistical description of the neighboring coefficients in a window. These methods however do not give good quality of the images since they cannot modify and remove too many small wavelet coefficients simultaneously due to the threshold. In this paper, a new image denoising method is proposed that shrinks the noisy coefficients using an adaptive threshold. Our method overcomes these drawbacks and it has better performance than the NeighShrink, IAWDMBNC, IWST, LAWF, WPTMWF, and AIDMT denoising methods.

  17. Study on Stratification Cultivation of Automobile after-sale Service Talents in Higher Vocational Colleges Based on AHP%基于AHP法的高职汽车售后服务人才分层培养研究

    Institute of Scientific and Technical Information of China (English)

    鲍婷婷

    2014-01-01

    汽车售后服务人才是影响汽车维修企业发展的核心要素。运用AHP法对汽车售后服务人才所需要的能力进行分析,从专业技能、社会技能和概念技能三个方面构建人才评价体系,并提出了相应的分层培养策略。%Automobile after-sale service talents are the key factor of the development of automobile repair enterprises. This paper an⁃alyzes the abilities required by automobile after-sale service talents based on AHP. The talents evaluating system is constructed from the three aspects of professional skills, social skills and conceptual skills, and corresponding tactics for stratification cultivation are put forward.

  18. Cultivation of English Comprehensive Competences Based on Students' Majors%基于专业人才培养定位的大学英语综合应用能力培养

    Institute of Scientific and Technical Information of China (English)

    李俊波

    2012-01-01

    本文从社会需要出发分析了专业人才的英语素质和大学英语综合应用能力培养过程中的问题,提出了搞好大学英语自身特色建设的建议,倡导基于专业人才培养定位的大学英语综合应用能力培养。%This paper analyses professional talents' English skills required from the view of society and work situation as well as the problems in the process of college English education with regard to students' English comprehensive competences. It puts forward sev- eral suggestions to improve College English education, proposing cultivation of English comprehensive competences based on students' majors

  19. A New Method for Riccati Differential Equations Based on Reproducing Kernel and Quasilinearization Methods

    Directory of Open Access Journals (Sweden)

    F. Z. Geng

    2012-01-01

    Full Text Available We introduce a new method for solving Riccati differential equations, which is based on reproducing kernel method and quasilinearization technique. The quasilinearization technique is used to reduce the Riccati differential equation to a sequence of linear problems. The resulting sets of differential equations are treated by using reproducing kernel method. The solutions of Riccati differential equations obtained using many existing methods give good approximations only in the neighborhood of the initial position. However, the solutions obtained using the present method give good approximations in a larger interval, rather than a local vicinity of the initial position. Numerical results compared with other methods show that the method is simple and effective.

  20. 白假丝酵母菌简便培养方法的建立及临床应用研究%The constrution and clinical application of convenient Candida albicans cultivation method

    Institute of Scientific and Technical Information of China (English)

    王晓玲; 侯佳宜; 高颖; 杨少芳; 任建平

    2014-01-01

    目的:探讨真菌简便培养方法的临床应用价值。方法收集2006年1月-2013年12月我院门诊及住院患者疑似真菌感染标本,建立真菌简便培养基对收集标本进行培养,并将其与法国梅里埃培养基进行比较;对比新鲜自制简便培养基与2~8℃放置6个月后简便培养基的真菌检出率。结果在2011-2013年收集的307份标本中,简便培养基白假丝酵母菌的检出率为38.4%,而梅里埃培养基的检出率为26.1%,自制简便培养基对阴道分泌物标本、痰标本白假丝酵母菌检出率优于梅里埃培养基,且差异均有统计学意义(P均<0.05),而尿标本和咽拭子标本中两种方法白假丝酵母菌的检出率差异均无统计学意义(P均>0.05)。白假丝酵母菌在两种培养基上的菌落形态相似,但梅里埃培养基上白假丝酵母菌菌落显示蓝色,而自制简便培养基白假丝酵母菌菌落呈奶油色,并且均能抑制大部分杂菌的生长。痰标本、阴道分泌物标本及其他标本的白假丝酵母菌检出率在新鲜与2~8℃放置6个月后培养基中差异均无统计学意义(P均>0.05)。在2006-2010年共2840份阴道分泌物标本中,检出白假丝酵母菌以外的其他致病菌475份,阳性检出率为16.7%(475/2840),其中白假丝酵母菌与其他致病菌双重感染者有87例,阳性检出率为3.1%(87/2840)。结论真菌简便培养基制作简单、成本低廉,对白假丝酵母菌有良好选择性,可向农村基层医疗机构推广使用。%Objective To study the clinical application value of a convenient fungus cultivation method. Methods The suspected samples of fungus infection from the clinic service and hospitalization pa-tients in our hospital from January 2006 to December 2013 were collected. The fungus infection samples were detected by convenient fungus cultivation method. The cultivated results of homemade

  1. 大鼠骨髓间充质干细胞的分离方法和培养%Rat bone marrow mesenchymal stem cells‘s separation methods and to cultivate

    Institute of Scientific and Technical Information of China (English)

    邹瑾; 李雅; 尹进

    2015-01-01

    目的:探索在体外培养和纯化SD大鼠的骨髓间充质干细胞(BMSCs)的方法。方法:采用改良全骨髓贴壁方法,分离和纯化3~4周的大鼠BMSCs,镜下连续观察细胞的形态变化。流式细胞仪鉴定其表面抗原CD11b、CD45和CD90的表达情况。结果:原代培养的细胞呈圆形和梭形等,24小时后大部分细胞均贴壁。8~10天可达80%~90%融合,纯化后传代周期为6~8天。流式细胞术鉴定表明CD11b和CD45阴性,CD90阳性。结论:改良全骨髓贴壁法可有效分离培养大鼠的骨髓间充质干细胞,是一种比较理想的分离培养方法。%Objective To explore the in vitro culture and purification of SD rat bone marrow mesenchymal stem cells (BMSCs) method.Methods Using improved the whole bone marrow adherent method, separation and purification of rat (for 3~ 4 weeks )of BMSCs, microscopically continuous observation of cell morphology change. To identification cells surface antigen CD11b, CD45 and CD90 expression using flow cytometry.Results The original generation of cultured cells is circular and spindle, after 24 hours, most of the cells are attached. 8~10 days later, the fusion of up to 80%~ 90%. After purification,extend the period is 6~ 8 days. Using flow cytometry assay, CD11b and CD45 were negative, CD90 is positive.ConclusionsImproved the whole bone marrow adherent method, which can effectively the isolation and culture of rat bone marrow mesenchymal stem cells, is an ideal method of cultivation.

  2. Forest cover dynamics of shifting cultivation in the Democratic Republic of Congo: a remote sensing-based assessment for 2000-2010

    Science.gov (United States)

    Molinario, G.; Hansen, M. C.; Potapov, P. V.

    2015-09-01

    Shifting cultivation has traditionally been practiced in the Democratic Republic of Congo by carving agricultural fields out of primary and secondary forest, resulting in the rural complex: a characteristic land cover mosaic of roads, villages, active and fallow fields and secondary forest. Forest clearing has varying impacts depending on where it occurs relative to this area: whether inside it, along its primary forest interface, or in more isolated primary forest areas. The spatial contextualization of forest cover loss is therefore necessary to understand its impacts and plan its management. We characterized forest clearing using spatial models in a Geographical Information System, applying morphological image processing to the Forets d’Afrique Central Evaluee par Teledetection product. This process allowed us to create forest fragmentation maps for 2000, 2005 and 2010, classifying previously homogenous primary forest into separate patch, edge, perforated, fragmented and core forest subtypes. Subsequently we used spatial rules to map the established rural complex separately from isolated forest perforations, tracking the growth of these areas in time. Results confirm that the expansion of the rural complex and forest perforations has high variance throughout the country, with consequent differences in local impacts on forest ecology and habitat fragmentation. Between 2000 and 2010 the rural complex grew by 10.2% (46 182 ha), increasing from 11.9% to 13.1% of the total land area (1.2% change) while perforated forest grew by 74.4% (23 856 ha), from 0.8% to 1.5%. Core forest decreased by 3.8% (54 852 ha), from 38% to 36.6% of the 2010 land area. Of particular concern is the nearly doubling of perforated forest, a land dynamic that represents greater spatial intrusion of forest clearing within core forest areas and a move away from the established rural complex.

  3. A Fast Leak Locating Method Based on Wavelet Transform

    Institute of Scientific and Technical Information of China (English)

    GE Chuanhu; YANG Hongying; YE Hao; WANG Guizeng

    2009-01-01

    The problem of leak location is actually a time delay estimation (TDE) problem. Since most exist-ing TDE methods may encounter the problem of high computational complexity when used for online leak location. This paper presents a fast leak locating method based on wavelet transform (WT). The method first gets a rough estimate of the time delay from the WT coefficients of the pressure signals at the largest scale, then keeps refining the estimate using WT coefficients on smaller and smaller scales. Quantitative analyses and test results based on real data show that the method reduces the computational complexity while main-taining the time delay estimation accuracy.

  4. Network Traffic Anomalies Identification Based on Classification Methods

    Directory of Open Access Journals (Sweden)

    Donatas Račys

    2015-07-01

    Full Text Available A problem of network traffic anomalies detection in the computer networks is analyzed. Overview of anomalies detection methods is given then advantages and disadvantages of the different methods are analyzed. Model for the traffic anomalies detection was developed based on IBM SPSS Modeler and is used to analyze SNMP data of the router. Investigation of the traffic anomalies was done using three classification methods and different sets of the learning data. Based on the results of investigation it was determined that C5.1 decision tree method has the largest accuracy and performance and can be successfully used for identification of the network traffic anomalies.

  5. Data Mining and Knowledge Discovery via Logic-Based Methods

    CERN Document Server

    Triantaphyllou, Evangelos

    2010-01-01

    There are many approaches to data mining and knowledge discovery (DM&KD), including neural networks, closest neighbor methods, and various statistical methods. This monograph, however, focuses on the development and use of a novel approach, based on mathematical logic, that the author and his research associates have worked on over the last 20 years. The methods presented in the book deal with key DM&KD issues in an intuitive manner and in a natural sequence. Compared to other DM&KD methods, those based on mathematical logic offer a direct and often intuitive approach for extracting easily int

  6. 医学院校学生的跨文化交际能力培养策略探析--基于主题式单元教学模式%A study on cultivating strategy of medical students’ intercultural communication competence at college level---Based on the theme-based unit teaching

    Institute of Scientific and Technical Information of China (English)

    褚宏蕊; 金文

    2013-01-01

    In the context of globalization, medical personnel with high intercultural communicative competence has become necessary of social development, this paper compares the differences between the traditional teaching mode and the theme of unit teaching mode, analyzes the inevitable demand of the cultivation of intercultural communicative competence, put forward methods of intercultural communicative competence of medical students training thematic unit based on the teaching mode in English teaching in university, to pay attention to groups of scholars concern and education and medical students of intercultural communicative competence.%在全球化的大背景下,具有较高跨文化交际能力的医学人才也成为社会发展的必需,本文通过对比传统教学模式与主题式单元教学模式的差异,分析培养跨文化交际能力的必然需求,提出在大学英语教学中基于主题式单元教学模式培养医学生的跨文化交际能力的方法,旨在引起学者对此问题的关注以及教育者及医学生群体对跨文化交际能力的重视。

  7. 不同耕作方式对土壤物理性状及玉米产量的影响%Effects of Different Cultivation Methods on the Soil Physical Properties and Yield of Maize

    Institute of Scientific and Technical Information of China (English)

    吕巨智; 程伟东; 钟昌松; 范继征; 石达金; 刘永红; 闫飞燕

    2014-01-01

    The purpose of this experiment was to identify the best cultivation method of maize and provide references for enhancing yield of maize cultivars. To study the effects of different tillage methods on soil physical properties and yield with different growing stages, a field experiment was conducted with randomized block design. The effects of different tillage methods on soil moisture content, bulk density, porosity in 0-45 cm soil depth varied with different growing stages. Among all the 6 tillage methods, the effect of 25-35 cm subsoiling depth on those physical properties mentioned above was the most obvious. In addition, after the subsoiling treatment, maize yield components, such as corn ear length, ear diameter, barren-tip length, number of rows per ear, kernels per row and 100-grain weight were improved at different degrees compared with CK. The maize yield with subsoiling treatment was 0.06%-3.13%higher than CK, but not significantly. Subsoiling could increase soil moisture, decrease soil bulk density, increase the porosity, function as water conservation and increase maize yield. Among them, the combination of 35 cm subsoiling depth and rotary tillage was the most optimal.%为寻求最佳耕作方式、提高玉米品种产量提供理论依据,采用随机区组设计,研究6种不同耕作方式对土壤物理性状和玉米产量的影响。不同耕作方式对不同生育时期0~45 cm土壤的含水量、容重、孔隙度影响各不同,在不同耕作方式中以深松25~35 cm效果最为明显;深松处理的玉米穗长、穗粗、秃尖长、穗行数、行粒数、百粒重等产量构成因素均较对照有不同程度的改善;深松处理比对照玉米增产0.06%~3.13%,但是增产幅度不是很明显。深松处理可以提高土壤含水量,降低容重,增加孔隙度,起到蓄水保墒作用。以深松25~35 cm为最优组合。

  8. Breeding management method of long-term artificial cultivation of tree shrew in beijing area%北京地区实验树鼩的饲养管理方法

    Institute of Scientific and Technical Information of China (English)

    赵琰; 吴婷婷; 李翼飞; 孙晔; 孙慧; 胡丽娜; 屈会化; 王庆国

    2013-01-01

    树鼩作为低等灵长类动物,在生物学研究中的价值日益引起研究者重视,将其作为实验动物的实验需求越来越多.作为南方亚热带动物,树鼩如何适应北方地区的生活是北方地区将其用作实验动物的前提.本实验室探索出适宜树鼩居住的饲养笼具,确定了饲养环境温度、湿度、光照时间、噪声及通风等条件,制定了规范化的卫生管理标准,建立了北京地区长期人工培育树鼩的饲养管理方法,为今后利用树鼩进行中医药病证结合动物模型的研究奠定了基础.%Tree shrew, a species of lower primates, its value in biomedical research has draw attention of researchers, and have been more and more demanded as a laboratory animal. However, tree shrew as a southern subtropical animal, how to adapt it to live in northern China becomes the premise that tree shrew can be used as a laboratory animal in northern China. Our laboratory has explored feeding cage, which is suitable for tree shrew living, determined feeding environmental conditions, such as temperature, humidity, illumination time, noise, ventilation, etc. , formulated the health management standard and established breeding management method of long-term artificial cultivation of tree shrew in Beijing area. Therefore, we have laid the foundation for the application of tree shrew in research on traditional Chinese medicine syndrome combined with establishment of disease animal models in the future.

  9. Ellipse-based shape description and retrieval method

    Institute of Scientific and Technical Information of China (English)

    李向阳; 潘云鹤

    2002-01-01

    Using a group of ellipses to approach the shape contour, a new shape retrieval method is presented in this paper. In order to keep shape-based retrieval invariant to its position, orientation and size, the shape normalization method is presented. From our research, any closed shape contour can be uniquely decomposed into a group of ellipses, and the original shape contour can be re-constructed using the decomposed ellipses. The ellipse-based shape description and similar retrieval method is introduced in this paper. Based on ellipse's contribution to shape contour, the decomposed ellipses are parted into low-order ellipses and high-order ellipses. The low-order ellipses measure the macroscopic feature of a shape contour, and the high-order ellipses measure the microscopic feature. The two-phase shape matching method is given. Through the experiment test, our method has better shape retrieval effect.

  10. ENHANCED GRAPH BASED NORMALIZED CUT METHODS FOR IMAGE SEGMENTATION

    Directory of Open Access Journals (Sweden)

    S.D. Kapade

    2014-11-01

    Full Text Available Image segmentation is one of the important steps in digital image processing. Several algorithms are available for segmenting the images, posing many challenges such as precise criteria and efficient computations. Most of the graph based methods used for segmentation depend on local properties of graphs without considering global impressions of image, which ultimately limits segmentation quality. In this paper, we propose an enhanced graph based normalized cut method for extracting global impression and consistencies in the image. We propose a technique to add flexibility to original recursive normalized two way cut method which was further extended to other graph based methods. The results show that the proposed technique improves segmentation quality as well as requires lesser computational time than the regular normalized cut method.

  11. Evaluation of PCR-based beef sexing methods.

    Science.gov (United States)

    Zeleny, Reinhard; Bernreuther, Alexander; Schimmel, Heinz; Pauwels, Jean

    2002-07-17

    Analysis of the sex of beef meat by fast and reliable molecular methods is an important measure to ensure correct allocation of export refunds, which are considerably higher for male beef meat. Two PCR-based beef sexing methods have been optimized and evaluated. The amelogenin-type method revealed excellent accuracy and robustness, whereas the bovine satellite/Y-chromosome duplex PCR procedure showed more ambiguous results. In addition, an interlaboratory comparison was organized to evaluate currently applied PCR-based sexing methods in European customs laboratories. From a total of 375 samples sent out, only 1 false result was reported (female identified as male). However, differences in the performances of the applied methods became apparent. The collected data contribute to specify technical requirements for a common European beef sexing methodology based on PCR. PMID:12105941

  12. ENHANCED GRAPH BASED NORMALIZED CUT METHODS FOR IMAGE SEGMENTATION

    OpenAIRE

    S.D. Kapade; S.M. Khairnar; B.S. Chaudhari

    2014-01-01

    Image segmentation is one of the important steps in digital image processing. Several algorithms are available for segmenting the images, posing many challenges such as precise criteria and efficient computations. Most of the graph based methods used for segmentation depend on local properties of graphs without considering global impressions of image, which ultimately limits segmentation quality. In this paper, we propose an enhanced graph based normalized cut method for extracting global imp...

  13. Convergence of a residual based artificial viscosity finite element method

    KAUST Repository

    Nazarov, Murtazo

    2013-02-01

    We present a residual based artificial viscosity finite element method to solve conservation laws. The Galerkin approximation is stabilized by only residual based artificial viscosity, without any least-squares, SUPG, or streamline diffusion terms. We prove convergence of the method, applied to a scalar conservation law in two space dimensions, toward an unique entropy solution for implicit time stepping schemes. © 2012 Elsevier B.V. All rights reserved.

  14. Dynamics of decadal changes in the distribution of double-cropping rice cultivation in China

    Institute of Scientific and Technical Information of China (English)

    DUAN JuQi; ZHOU GuangSheng

    2013-01-01

    Quantitative description of changes in the distribution of paddy rice cultivation in response to recent climate change provides a reference for rice cultivation patterns and formulation of countermeasures to cope with future climate change in China.This study analyzes the dynamics of decadal changes in distribution of double-cropping rice in China during 1961-2010 in relation to climate change based on the maximum entropy method.Decadal changes in the double-cropping rice cultivation area and climatic suitability in China were apparent.The total area of climatically suitable regions was highest in the 1960s,and subsequently showed an increasing trend at first and then a decreasing trend from the 1970s to 2000s.However,the low climatic suitability area decreased,which implied that the moderate and high climatic suitability areas increased.Among the latter,the high climatic suitability area showed the highest increase in extent to 4.4 times that of the 1990s and four times that of the 1960s.The areas of double-cropping rice cultivation most sensitive to climate change are mainly located in central Jiangsu,central Anhui,the eastern Sichuan Basin,southern Henan and central Guizhou.Transformation of areas between low and moderate climatic suitability was observed in northern Zhejiang,southern Anhui and Hubei,and northern Guangxi.Transformation of areas between moderate and high climatic suitability was observed in central Jiangxi and Leizhou Peninsula.The northern boundary of double-cropping rice cultivation in China shifted southwards and contracted eastwards in the 1970s,and extended northwards in the 1980s.However,the northern boundary did not shift northwards in response to climate warming in the 2000s.

  15. Genetic structure in cultivated and wild carrots (¤Daucus carota¤ L.) revealed by AFLP analysis

    DEFF Research Database (Denmark)

    Shim, S.I.; Bagger Jørgensen, Rikke

    2000-01-01

    Genetic variation within and among five Danish populations of wild carrot and five cultivated varieties was investigated using amplified fragment length polymorphism (AFLP). Ten AFLP primer combinations produced 116 polymorphic bands. Based on the marker data an UPGMA-cluster analysis and principal...... markers specific to the cultivated carrot makes it possible to detect introgression from cultivated to wild types....

  16. A New Nonlinear Compound Forecasting Method Based on ANN

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    In this paper the compound-forecasting method is discussed. The compound-forecasting method is one of the hotspots in the current predication. Firstly, the compound-forecasting method is introduced and various existing compound-forecasting methods arediscussed. Secondly, the Artificial Neural Network (ANN) is brought in compound-prediction research and a nonlinear compound-prediction model based on ANN is presented. Finally, inorder to avoid irregular weight, a new method is presented which uses principal component analyses to increase the availability of compound-forecasting information. Higherforecasting precision is achieved in practice.

  17. Competition assignment problem algorithm based on Hungarian method

    Institute of Scientific and Technical Information of China (English)

    KONG Chao; REN Yongtai; GE Huiling; DENG Hualing

    2007-01-01

    Traditional Hungarian method can only solve standard assignment problems, while can not solve competition assignment problems. This article emphatically discussed the difference between standard assignment problems and competition assignment problems. The kinds of competition assignment problem algorithms based on Hungarian method and the solutions of them were studied.

  18. Nonparametric forecasting: a comparison of three kernel-based methods

    NARCIS (Netherlands)

    J.G. de Gooijer; E. Matzner-Lober; A. Gannoun

    1998-01-01

    In this paper the use of three kernel-based nonparametric forecasting methods - the conditional mean, the conditional median, and the conditioal mode - is explored in detail. Several issues related to the estimation of these methods are discussed, including the choice of the bandwidth and the type o

  19. Tomographs based on non-conventional radiation sources and methods

    International Nuclear Information System (INIS)

    Computer techniques for tomographic reconstruction of objects X-rayed with a compact plasma focus (PF) are presented. The implemented reconstruction algorithms are based on stochastic searching of solutions of Radon equation, using Genetic Algorithms and Monte Carlo methods. Numerical experiments using actual projections were performed concluding the feasibility of the application of both methods in tomographic reconstruction problem. (author)

  20. The afforestation problem: a heuristic method based on simulated annealing

    DEFF Research Database (Denmark)

    Vidal, Rene Victor Valqui

    1992-01-01

    This paper presents the afforestation problem, that is the location and design of new forest compartments to be planted in a given area. This optimization problem is solved by a two-step heuristic method based on simulated annealing. Tests and experiences with this method are also presented....

  1. A CT Image Segmentation Algorithm Based on Level Set Method

    Institute of Scientific and Technical Information of China (English)

    QU Jing-yi; SHI Hao-shan

    2006-01-01

    Level Set methods are robust and efficient numerical tools for resolving curve evolution in image segmentation. This paper proposes a new image segmentation algorithm based on Mumford-Shah module. The method is used to CT images and the experiment results demonstrate its efficiency and veracity.

  2. A Channelization-Based DOA Estimation Method for Wideband Signals

    Directory of Open Access Journals (Sweden)

    Rui Guo

    2016-07-01

    Full Text Available In this paper, we propose a novel direction of arrival (DOA estimation method for wideband signals with sensor arrays. The proposed method splits the wideband array output into multiple frequency sub-channels and estimates the signal parameters using a digital channelization receiver. Based on the output sub-channels, a channelization-based incoherent signal subspace method (Channelization-ISM and a channelization-based test of orthogonality of projected subspaces method (Channelization-TOPS are proposed. Channelization-ISM applies narrowband signal subspace methods on each sub-channel independently. Then the arithmetic mean or geometric mean of the estimated DOAs from each sub-channel gives the final result. Channelization-TOPS measures the orthogonality between the signal and the noise subspaces of the output sub-channels to estimate DOAs. The proposed channelization-based method isolates signals in different bandwidths reasonably and improves the output SNR. It outperforms the conventional ISM and TOPS methods on estimation accuracy and dynamic range, especially in real environments. Besides, the parallel processing architecture makes it easy to implement on hardware. A wideband digital array radar (DAR using direct wideband radio frequency (RF digitization is presented. Experiments carried out in a microwave anechoic chamber with the wideband DAR are presented to demonstrate the performance. The results verify the effectiveness of the proposed method.

  3. A Channelization-Based DOA Estimation Method for Wideband Signals.

    Science.gov (United States)

    Guo, Rui; Zhang, Yue; Lin, Qianqiang; Chen, Zengping

    2016-01-01

    In this paper, we propose a novel direction of arrival (DOA) estimation method for wideband signals with sensor arrays. The proposed method splits the wideband array output into multiple frequency sub-channels and estimates the signal parameters using a digital channelization receiver. Based on the output sub-channels, a channelization-based incoherent signal subspace method (Channelization-ISM) and a channelization-based test of orthogonality of projected subspaces method (Channelization-TOPS) are proposed. Channelization-ISM applies narrowband signal subspace methods on each sub-channel independently. Then the arithmetic mean or geometric mean of the estimated DOAs from each sub-channel gives the final result. Channelization-TOPS measures the orthogonality between the signal and the noise subspaces of the output sub-channels to estimate DOAs. The proposed channelization-based method isolates signals in different bandwidths reasonably and improves the output SNR. It outperforms the conventional ISM and TOPS methods on estimation accuracy and dynamic range, especially in real environments. Besides, the parallel processing architecture makes it easy to implement on hardware. A wideband digital array radar (DAR) using direct wideband radio frequency (RF) digitization is presented. Experiments carried out in a microwave anechoic chamber with the wideband DAR are presented to demonstrate the performance. The results verify the effectiveness of the proposed method. PMID:27384566

  4. Pupils Humanistic Qualities Cultivating Based on Reading and Teaching of Children′s Literature%基于儿童文学阅读教学的小学生人文素养培养

    Institute of Scientific and Technical Information of China (English)

    程雯; 程瑞

    2015-01-01

    For the implementation of"rule system"in children′s literature humanistic qualities,it pointed out that must comply with the development regular of children′s cognitive psychology.The specific strategies were as follows,combining to interactive between home and school,creating reading environment;cultivating reading habits,feeling reading pleasure;strengthening the guideness of methods,building reading system;highlighting teaching characteristics,combining in class with out of class;subliming"facts judgment",understanding human feelings.The practical results of M and N school confirmed that reading and teaching of children′s literature is an effective way to cultivate pupil humanistic qualities.%针对儿童文学人文素养中“规则体系”的实施,指出其必须符合儿童认知心理发展规律。具体策略是:家校联合互动、营造阅读氛围;培养阅读习惯、感受阅读快乐;加强方法指导、构建阅读体系;凸显教学特色、课堂内外结合;升华“事实判断”、感悟人文情怀。 M、N校的实践结果印证了儿童文学阅读教学是培养小学生人文素养的有效途径。

  5. A Semantic Retrieval Method Based on the Fuzzy Reasoning

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    This paper gives a semantic fuzzy retrieval method of multimedia object,discusses the principle of fuzzy semantic retrieval technique,presents a fuzzy reasoning mechanism based on the knowledge base,and designs the relevant reasoning algorithms.Researchful results have innovative significance.

  6. Role-based Integration Method of Enterprise Information System

    Institute of Scientific and Technical Information of China (English)

    YU Ming-hui; FEI Qi; CHEN Xue-guang

    2002-01-01

    This paper analyzes the current situation of enterprise information system and methods of system integration at first. Then a role-based analyzing method is proposed. It can help confirm the keystone of the construction of information system and the direction of system integration. At last, a case study on theintegration of material dispatching information system in a large-scale project is presented briefly. It shows that this new method is more effective than the others are.

  7. A New Video Coding Method Based on Improving Detail Regions

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The Moving Pictures Expert Group (MPEG) and H.263 standard coding method is widely used in video compression. However, the visual quality of detail regions such as eyes and mouth is not content in people at the decoder, as far as the conference telephone or videophone is concerned. A new coding method based on improving detail regions is presented in this paper. Experimental results show that this method can improve the visual quality at the decoder.

  8. On the Cultivation of Chinese Animation Industry Based on Crowd-funding Business Model%基于众筹商业模式的中国动漫产业培育研究

    Institute of Scientific and Technical Information of China (English)

    李也; 贺正楚; 潘红玉

    2015-01-01

    众筹作为一种企业融资模式,在国外被应用于文化产品的项目开发。从众筹商业模式的分析框架出发,借鉴国外成功经验,揭示中国动漫产业发展过程中的困境,探讨和创新我国动漫产业的培育模式。基于众筹商业模式的动漫产业培育研究,为动漫企业开发产品的经营方式,以及政府出台扶持动漫产业的政策,提供了新的理论参考和政策依据。%As a corporation financing model, Crowd-funding has been applied to develop cultural products in the western countries.This essay analyses the framework of the Crowd-funding business model and learns from the successful experience of for-eign countries, revealing the difficulties at the development of China’ s animation industry, discussing and updating cultivation pat-tern of China’ s animation industry.Based on the Crowd-funding business model, the cultivation study of animation industry provides a new theoretical reference and policy basis for the operation of the animation business product development as well as the support of the government policy.

  9. A simple procedure for preparing substrate for Pleurotus ostreatus cultivation.

    Science.gov (United States)

    Hernández, Daniel; Sánchez, José E; Yamasaki, Keiko

    2003-11-01

    The use of wooden crates for composting a mixture of 70% grass, (Digitaria decumbens), and 30% coffee pulp, combined with 2% Ca(OH)(2), was studied as a method for preparing substrate for the cultivation of Pleurotus ostreatus. Crate composting considerably modified the temperature pattern of the substrate in process, as compared to pile composting, where lower temperatures and less homogeneous distributions were observed. Biological efficiencies varied between 59.79% and 93% in the two harvests. Based on statistical analysis significant differences were observed between the treatments, composting times and in the interactions between these two factors. We concluded that it is possible to produce P. ostreatus on a lignocellulosic, non-composted, non-pasteurized substrate with an initial pH of 8.7, and that composting for two to three days improves the biological efficiency.

  10. A method for selecting training samples based on camera response

    Science.gov (United States)

    Zhang, Leihong; Li, Bei; Pan, Zilan; Liang, Dong; Kang, Yi; Zhang, Dawei; Ma, Xiuhua

    2016-09-01

    In the process of spectral reflectance reconstruction, sample selection plays an important role in the accuracy of the constructed model and in reconstruction effects. In this paper, a method for training sample selection based on camera response is proposed. It has been proved that the camera response value has a close correlation with the spectral reflectance. Consequently, in this paper we adopt the technique of drawing a sphere in camera response value space to select the training samples which have a higher correlation with the test samples. In addition, the Wiener estimation method is used to reconstruct the spectral reflectance. Finally, we find that the method of sample selection based on camera response value has the smallest color difference and root mean square error after reconstruction compared to the method using the full set of Munsell color charts, the Mohammadi training sample selection method, and the stratified sampling method. Moreover, the goodness of fit coefficient of this method is also the highest among the four sample selection methods. Taking all the factors mentioned above into consideration, the method of training sample selection based on camera response value enhances the reconstruction accuracy from both the colorimetric and spectral perspectives.

  11. Method of designing developable surface based on engineering requirement

    Institute of Scientific and Technical Information of China (English)

    YANG Ji-xin; LIU Zhe; LIU Jian

    2006-01-01

    The paper deals with the principle of envelope of a one-parameter plane family to design developable surfaces. Three methods of designing developable surfaces are presented. They are designing a developable surface based on one curve on it and its normal line, designing a developable surface based on two curves on it and designing a developable surface based on one curve and one surface. They meet the requirements of engineering fields.

  12. An Efficient Method for Reliability-based Multidisciplinary Design Optimization

    Institute of Scientific and Technical Information of China (English)

    Fan Hui; Li Weiji

    2008-01-01

    Design for modem engineering system is becoming multidisciplinary and incorporates practical uncertainties; therefore, it is necessary to synthesize reliability analysis and the multidiscipLinary design optimization (MDO) techniques for the design of complex engineering system. An advanced first order second moment method-based concurrent subspace optimization approach is proposed based on the comparison and analysis of the existing multidisciplinary optimization techniques and the reliability analysis methods. It is seen through a canard configuration optimization for a three-surface transport that the proposed method is computationally efficient and practical with the least modification to the current deterministic optimization process.

  13. Impacts of recent cultivation on genetic diversity pattern of a medicinal plant, Scutellaria baicalensis (Lamiaceae

    Directory of Open Access Journals (Sweden)

    Shao Ai-Juan

    2010-04-01

    Full Text Available Abstract Background Cultivation of medicinal plants is not only a means for meeting current and future demands for large volume production of plant-based drug and herbal remedies, but also a means of relieving harvest pressure on wild populations. Scutellaria baicalensis Georgi (Huang-qin or Chinese skullcap is a very important medicinal plant in China. Over the past several decades, wild resource of this species has suffered rapid declines and large-scale cultivation was initiated to meet the increasing demand for its root. However, the genetic impacts of recent cultivation on S. baicalensis have never been evaluated. In this study, the genetic diversity and genetic structure of 28 wild and 22 cultivated populations were estimated using three polymorphic chloroplast fragments. The objectives of this study are to provide baseline data for preserving genetic resource of S. baicalensis and to evaluate the genetic impacts of recent cultivation on medicinal plants, which may be instructive to future cultivation projects of traditional Chinese medicinal plants. Results Thirty-two haplotypes of S. baicalensis (HapA-Y and Hap1-7 were identified when three chloroplast spacers were combined. These haplotypes constituted a shallow gene tree without obvious clusters for cultivated populations, suggesting multiple origins of cultivated S. baicalensis. Cultivated populations (hT = 0.832 maintained comparable genetic variation with wild populations (hT = 0.888, indicating a slight genetic bottleneck due to multiple origins of cultivation. However, a substantial amount of rare alleles (10 out of 25 haplotypes within wild populations lost during the course of S. baicalensis cultivation. The genetic differentiation for cultivated group (GST = 0.220 was significantly lower than that of wild group (GST = 0.701. Isolation by distance analysis showed that the effect of geographical isolation on genetic structure was significant in wild populations (r = 0.4346, P r

  14. 建设病案管理实训基地与创新人才培养模式的探讨%Construction of Medical Record Information Management Training Base and Discussion of Innovative Talents Cultivation

    Institute of Scientific and Technical Information of China (English)

    王宁; 邢春国; 段沁江; 徐芬; 吴良明

    2016-01-01

    Medical record information management is a key link in modern hospital management, medical record information management level directly affects the process of health care reform, the lagging development of medical record management in our country, the shortage of medical record management personnel, to cultivate high quality medical record information management talent is imminent. In this paper, through literature search, questionnaire survey method,in view of the medical institutions for medical record information management personnel diathesis and vocational skills, medical record information management personnel in the core ability is put forward systematic medical record information practice base construction plan, including field condition, equipment requirements, the informationization level, management mechanism, teaching organization and operation management, etc. Through the establishment of cooperation professional training base construction, teaching reform of collaboration, cooperation, cooperation practice and employment mechanism of university-enterprise cooperation, to create "fusion, learn to do fusion, the fusion" personnel training mode, improve students' professional quality of medical record information management, to meet the requirements of health informatization level continuously improve and meet the requirements of medical and health care reform.%病案信息管理是现代医院管理中的重要环节,病案信息管理的水平直接影响着医疗卫生改革的进程,我国病案管理发展滞后,病案管理人才队伍紧缺,培养高素质的病案信息管理人才迫在眉睫.通过文献检索,问卷调查等方法,针对医疗机构对病案信息管理人才素质要求及职业技能目标,围绕病案信息管理人才核心能力提出系统化病案信息实训基地建设方案,包括场地条件、设备要求、信息化水平、管理机制、教学组织和运行管理等.通过建立合作专业建设、合作教

  15. Cultivating the thinking mode of evidence-based medicine for retinal postgraduate students%循证医学实践与眼底病专业研究生循证思维的培养

    Institute of Scientific and Technical Information of China (English)

    张新媛

    2012-01-01

    With the developments in the fields of medicine and biomedicine, physicians are not only required to have a high level of clinical skills, but more emphased to have understanding, analysis, synthesis, inferring and practicing abilities to adapt to the needs of modern medicine. The modern evidence-based practice emphasizes the "student-based, problem-based" educational model. Raising clinical questions using the PICOT (population, intervention, comparator, outcome, time) principle is extremely important for the medical graduates in their clinical practice training, especially for the retinal graduate students. Utilizing the evidence-based medicine resources as much as they can, practicing actively, cultivating the science of clinical thinking are all necessary. Cultivating the evidence-based thinking mode has special significance for those who want to be an excellent retinal specialist. (Ophthalmol CHN, 2012, 21: 357-359)%面对医学及生物医药领域发展的突飞猛进,循证医学不仅仅强调医生具有高水平的临床技能,更多地强调其具备认识、分析、综合、推断总结和实践的多种能力,以适应现代医学的需要.现代循证医学教育的模式强调“以学生为中心,以问题为中心”.眼底病专科学位研究生临床实践的培养应从平日的临床教育及工作中注意以人群、干预、对照、结果、时间(population,intervention,comparator,outcome,time,PICOT)原则凝练临床问题,注重学习利用循证医学资源,积极实践,在临床实践中培养科学的临床思维,以备在今后眼底病的临床及科研工作中发挥优势.培养良好的循证思维对于一名眼底病医师的成长具有深远意义.

  16. Mapping cultivable land from satellite imagery with clustering algorithms

    Science.gov (United States)

    Arango, R. B.; Campos, A. M.; Combarro, E. F.; Canas, E. R.; Díaz, I.

    2016-07-01

    Open data satellite imagery provides valuable data for the planning and decision-making processes related with environmental domains. Specifically, agriculture uses remote sensing in a wide range of services, ranging from monitoring the health of the crops to forecasting the spread of crop diseases. In particular, this paper focuses on a methodology for the automatic delimitation of cultivable land by means of machine learning algorithms and satellite data. The method uses a partition clustering algorithm called Partitioning Around Medoids and considers the quality of the clusters obtained for each satellite band in order to evaluate which one better identifies cultivable land. The proposed method was tested with vineyards using as input the spectral and thermal bands of the Landsat 8 satellite. The experimental results show the great potential of this method for cultivable land monitoring from remote-sensed multispectral imagery.

  17. Patch nearfield acoustic holography based on the equivalent source method

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    On the basis of nearfield acoustic holography (NAH) based on the equivalent source method (ESM), patch NAH based on the ESM is proposed. The method overcomes the shortcoming in the conventional NAH that the hologram surface should be larger than the source surface. It need not to discretize the whole source and its measurement need not to cover the whole source. The measurement may be performed over the region of interest, and the reconstruction can be done in the region directly. The method is flexible in applications, stable in computation, and very easy to implement. It has good potential applications in engineering. The nu- merical simulations show the invalidity of the conventional NAH based on the ESM and prove the validities of the proposed method for reconstructing a partial source and the regularization for reducing the error effect of the pressure measured on the hologram surface.

  18. Distance Based Method for Outlier Detection of Body Sensor Networks

    Directory of Open Access Journals (Sweden)

    Haibin Zhang

    2016-01-01

    Full Text Available We propose a distance based method for the outlier detection of body sensor networks. Firstly, we use a Kernel Density Estimation (KDE to calculate the probability of the distance to k nearest neighbors for diagnosed data. If the probability is less than a threshold, and the distance of this data to its left and right neighbors is greater than a pre-defined value, the diagnosed data is decided as an outlier. Further, we formalize a sliding window based method to improve the outlier detection performance. Finally, to estimate the KDE by training sensor readings with errors, we introduce a Hidden Markov Model (HMM based method to estimate the most probable ground truth values which have the maximum probability to produce the training data. Simulation results show that the proposed method possesses a good detection accuracy with a low false alarm rate.

  19. A HMM-Based Method for Vocal Fold Pathology Diagnosis

    Directory of Open Access Journals (Sweden)

    Vahid Majidnezhad

    2012-11-01

    Full Text Available Acoustic analysis is a proper method in vocal fold pathology diagnosis so that it can complement and in some cases replace the other invasive, based on direct vocal fold observations methods. There are different approaches for vocal fold pathology diagnosis. This paper presents a method based on hidden markov model which classifies speeches into two classes: the normal and the pathological. Two hidden markov models are trained based on these two classes of speech and then the trained models are used to classify the dataset. The proposed method is able to classify the speeches with an accuracy of 93.75%. The results of this algorithm provide insights that can help biologists and computer scientists design high-performance system for detection of vocal fold pathology diagnosis.

  20. Optimizing distance-based methods for large data sets

    Science.gov (United States)

    Scholl, Tobias; Brenner, Thomas

    2015-10-01

    Distance-based methods for measuring spatial concentration of industries have received an increasing popularity in the spatial econometrics community. However, a limiting factor for using these methods is their computational complexity since both their memory requirements and running times are in {{O}}(n^2). In this paper, we present an algorithm with constant memory requirements and shorter running time, enabling distance-based methods to deal with large data sets. We discuss three recent distance-based methods in spatial econometrics: the D&O-Index by Duranton and Overman (Rev Econ Stud 72(4):1077-1106, 2005), the M-function by Marcon and Puech (J Econ Geogr 10(5):745-762, 2010) and the Cluster-Index by Scholl and Brenner (Reg Stud (ahead-of-print):1-15, 2014). Finally, we present an alternative calculation for the latter index that allows the use of data sets with millions of firms.

  1. Patch nearfield acoustic holography based on the equivalent source method

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    On the basis of nearfield acoustic holography (NAH) based on the equivalent source method (ESM), patch NAH based on the ESM is proposed. The method overcomes the shortcoming in the conventional NAH that the hologram surface should be larger than the source surface. It need not to discretize the whole source and its measurement need not to cover the whole source. The measurement may be performed over the region of interest, and the reconstruction can be done in the region directly. The method is flexible in applications, stable in computation, and very easy to implement. It has good potential applications in engineering. The numerical simulations show the invalidity of the conventional NAH based on the ESM and prove the validities of the proposed method for reconstructing a partial source and the regularization for reducing the error effect of the pressure measured on the hologram surface.

  2. A new earthquake location method based on the waveform inversion

    CERN Document Server

    Wu, Hao; Huang, Xueyuan; Yang, Dinghui

    2016-01-01

    In this paper, a new earthquake location method based on the waveform inversion is proposed. As is known to all, the waveform misfit function is very sensitive to the phase shift between the synthetic waveform signal and the real waveform signal. Thus, the convergence domain of the conventional waveform based earthquake location methods is very small. In present study, by introducing and solving a simple sub-optimization problem, we greatly expand the convergence domain of the waveform based earthquake location method. According to a large number of numerical experiments, the new method expands the range of convergence by several tens of times. This allows us to locate the earthquake accurately even from some relatively bad initial values.

  3. Detection of microbial diversity in endocarditis using cultivation-independent molecular techniques

    DEFF Research Database (Denmark)

    Wolff, Tine Y; Moser, Claus Ernst; Bundgaard, Henning;

    2011-01-01

    Background: The aim of this study was to investigate whether the diagnosis of infective endocarditis (IE) could be improved using molecular tools in addition to standard microscopy and cultivation methods. Methods: Cultivation was performed on blood or tissue samples as recommended in the modified...

  4. Correction of Misclassifications Using a Proximity-Based Estimation Method

    Science.gov (United States)

    Niemistö, Antti; Shmulevich, Ilya; Lukin, Vladimir V.; Dolia, Alexander N.; Yli-Harja, Olli

    2004-12-01

    An estimation method for correcting misclassifications in signal and image processing is presented. The method is based on the use of context-based (temporal or spatial) information in a sliding-window fashion. The classes can be purely nominal, that is, an ordering of the classes is not required. The method employs nonlinear operations based on class proximities defined by a proximity matrix. Two case studies are presented. In the first, the proposed method is applied to one-dimensional signals for processing data that are obtained by a musical key-finding algorithm. In the second, the estimation method is applied to two-dimensional signals for correction of misclassifications in images. In the first case study, the proximity matrix employed by the estimation method follows directly from music perception studies, whereas in the second case study, the optimal proximity matrix is obtained with genetic algorithms as the learning rule in a training-based optimization framework. Simulation results are presented in both case studies and the degree of improvement in classification accuracy that is obtained by the proposed method is assessed statistically using Kappa analysis.

  5. Correction of Misclassifications Using a Proximity-Based Estimation Method

    Directory of Open Access Journals (Sweden)

    Shmulevich Ilya

    2004-01-01

    Full Text Available An estimation method for correcting misclassifications in signal and image processing is presented. The method is based on the use of context-based (temporal or spatial information in a sliding-window fashion. The classes can be purely nominal, that is, an ordering of the classes is not required. The method employs nonlinear operations based on class proximities defined by a proximity matrix. Two case studies are presented. In the first, the proposed method is applied to one-dimensional signals for processing data that are obtained by a musical key-finding algorithm. In the second, the estimation method is applied to two-dimensional signals for correction of misclassifications in images. In the first case study, the proximity matrix employed by the estimation method follows directly from music perception studies, whereas in the second case study, the optimal proximity matrix is obtained with genetic algorithms as the learning rule in a training-based optimization framework. Simulation results are presented in both case studies and the degree of improvement in classification accuracy that is obtained by the proposed method is assessed statistically using Kappa analysis.

  6. Cultivating archives: meanings and identities

    NARCIS (Netherlands)

    E. Ketelaar

    2012-01-01

    By cultivating archives through successive activations, people and communities define their identities. In these activations, the meanings of archives are constructed and reconstructed. Archives are not a static artifact imbued with the record creator’s voice alone, but a dynamic process involving a

  7. Mushroom Cultivation in South Korea

    Directory of Open Access Journals (Sweden)

    Mustafa Kemal Soylu

    2016-03-01

    Full Text Available Mushroom cultivation in South Korea is increasing fast last decades. Mushroom cultivation of South Korea is 173577 tones and South Korea gains 800 million dollars income annually. Different kind of mushroom species are cultivated and 31% enoki mushroom (Flammulina velutipes, 26% king oyster (Pleurotus eryngii, 26% oyster mushroom (Pleurotus ostreatus, 13% white buton mushroom (Agaricus bisporus and 4% rest of the total mushroom Lentinula edodes, Ganoderma lucidum, Phellinus vb. are produced in Korea. 764 ha mushroom growing area was used for mushroom cultivation. 31% of total growing area is oyster mushroom (Pleurotus ostreatus and 14% king oyster (Pleurotus eryngii, 6% winter mushroom (Flummulina velutipes, 16% white button mushroom (Agaricus bisporus, 3% Phellinus, 3% reishii (Ganoderma lucidum and 27% other mushrooms. Mushroom is consumed frequently in Korea and mushroom consumption per person is 4.2 kg. Growing on log culture (oak mushroom, reishi and Phellinus, growing on shelves (Agaricus bisporus and Pleurotus ostreatus and bottle culture (Flummulina velutipes, Pleurotus ostreatus, Pleurotus eryngii are commonly used growing systems.

  8. A Methodological Examination of Cultivation.

    Science.gov (United States)

    Rubin, Alan M.; And Others

    1988-01-01

    Considers two issues in cultivation research. Examines relationships between television exposure and positive statements of social perceptions, and tests a model of instrumental media uses and effects. Finds television exposure to be unrelated to social attitudes, while program selectivity is related to all social attitudes except interpersonal…

  9. Cultivating Imagination Across Boundaries

    DEFF Research Database (Denmark)

    Sproedt, Henrik; Heape, Chris

    2014-01-01

    a perspective that is based on complex processes of relating we argue that innovation can be otherwise seen as the emergence of new meaning that arises from the ongoing gesture and response interactions between those involved through the knowing, doing, making and relating of participatory inquiry...

  10. Integrated navigation method based on inertial navigation system and Lidar

    Science.gov (United States)

    Zhang, Xiaoyue; Shi, Haitao; Pan, Jianye; Zhang, Chunxi

    2016-04-01

    An integrated navigation method based on the inertial navigational system (INS) and Lidar was proposed for land navigation. Compared with the traditional integrated navigational method and dead reckoning (DR) method, the influence of the inertial measurement unit (IMU) scale factor and misalignment was considered in the new method. First, the influence of the IMU scale factor and misalignment on navigation accuracy was analyzed. Based on the analysis, the integrated system error model of INS and Lidar was established, in which the IMU scale factor and misalignment error states were included. Then the observability of IMU error states was analyzed. According to the results of the observability analysis, the integrated system was optimized. Finally, numerical simulation and a vehicle test were carried out to validate the availability and utility of the proposed INS/Lidar integrated navigational method. Compared with the test result of a traditional integrated navigation method and DR method, the proposed integrated navigational method could result in a higher navigation precision. Consequently, the IMU scale factor and misalignment error were effectively compensated by the proposed method and the new integrated navigational method is valid.

  11. NETWORK INTRUSION DETECTION METHOD BASED ON RS-MSVM

    Institute of Scientific and Technical Information of China (English)

    Xiao Yun; Han Chongzhao; Zheng Qinghua; Zhang Junjie

    2006-01-01

    A new method called RS-MSVM (Rough Set and Multi-class Support Vector Machine) is proposed for network intrusion detection. This method is based on rough set followed by MSVM for attribute reduction and classification respectively. The number of attributes of the network data used in this paper is reduced from 41 to 30 using rough set theory. The kernel function of HVDM-RBF (Heterogeneous Value Difference Metric Radial Basis Function), based on the heterogeneous value difference metric of heterogeneous datasets, is constructed for the heterogeneous network data. HVDM-RBF and one-against-one method are applied to build MSVM. DARPA (Defense Advanced Research Projects Agency) intrusion detection evaluating data were used in the experiment. The testing results show that our method outperforms other methods mentioned in this paper on six aspects: detection accuracy, number of support vectors, false positive rate, false negative rate, training time and testing time.

  12. Wind Turbine Gearbox Fault Diagnosis Method Based on Riemannian Manifold

    Directory of Open Access Journals (Sweden)

    Shoubin Wang

    2014-01-01

    Full Text Available As multivariate time series problems widely exist in social production and life, fault diagnosis method has provided people with a lot of valuable information in the finance, hydrology, meteorology, earthquake, video surveillance, medical science, and other fields. In order to find faults in time sequence quickly and efficiently, this paper presents a multivariate time series processing method based on Riemannian manifold. This method is based on the sliding window and uses the covariance matrix as a descriptor of the time sequence. Riemannian distance is used as the similarity measure and the statistical process control diagram is applied to detect the abnormity of multivariate time series. And the visualization of the covariance matrix distribution is used to detect the abnormity of mechanical equipment, leading to realize the fault diagnosis. With wind turbine gearbox faults as the experiment object, the fault diagnosis method is verified and the results show that the method is reasonable and effective.

  13. NONLINEAR DATA RECONCILIATION METHOD BASED ON KERNEL PRINCIPAL COMPONENT ANALYSIS

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    In the industrial process situation, principal component analysis (PCA) is a general method in data reconciliation.However, PCA sometime is unfeasible to nonlinear feature analysis and limited in application to nonlinear industrial process.Kernel PCA (KPCA) is extension of PCA and can be used for nonlinear feature analysis.A nonlinear data reconciliation method based on KPCA is proposed.The basic idea of this method is that firstly original data are mapped to high dimensional feature space by nonlinear function, and PCA is implemented in the feature space.Then nonlinear feature analysis is implemented and data are reconstructed by using the kernel.The data reconciliation method based on KPCA is applied to ternary distillation column.Simulation results show that this method can filter the noise in measurements of nonlinear process and reconciliated data can represent the true information of nonlinear process.

  14. Consistency-based ellipse detection method for complicated images

    Science.gov (United States)

    Zhang, Lijun; Huang, Xuexiang; Feng, Weichun; Liang, Shuli; Hu, Tianjian

    2016-05-01

    Accurate ellipse detection in complicated images is a challenging problem due to corruptions from image clutter, noise, or occlusion of other objects. To cope with this problem, an edge-following-based ellipse detection method is proposed which promotes the performances of the subprocesses based on consistency. The ellipse detector models edge connectivity by line segments and exploits inconsistent endpoints of the line segments to split the edge contours into smooth arcs. The smooth arcs are further refined with a novel arc refinement method which iteratively improves the consistency degree of the smooth arc. A two-phase arc integration method is developed to group disconnected elliptical arcs belonging to the same ellipse, and two constraints based on consistency are defined to increase the effectiveness and speed of the merging process. Finally, an efficient ellipse validation method is proposed to evaluate the saliency of the elliptic hypotheses. Detailed evaluation on synthetic images shows that our method outperforms other state-of-the-art ellipse detection methods in terms of effectiveness and speed. Additionally, we test our detector on three challenging real-world datasets. The F-measure score and execution time of results demonstrate that our method is effective and fast in complicated images. Therefore, the proposed method is suitable for practical applications.

  15. Current situation for cultivating reflective thinking in English reading instruction--The influence of teaching models

    Institute of Scientific and Technical Information of China (English)

    Meng Qinglan

    2013-01-01

    Reflective thinking plays an essential role in cultivating students’creative ability. It’s essential to cultivate this ability in the process of reading instruction,while the present traditional English teaching models hinder its development. This paper tries to analyze the current situation in cultivating students’reflective thinking in English reading instruction from the traditional teaching models,in the hope of refreshing the teachers’consciousness on their teaching methods.

  16. Indigenous floating cultivation : a sustainable agricultural practice in the wetlands of Bangladesh.

    OpenAIRE

    Isalm, T.; Atkins, P.J.

    2007-01-01

    Floating-bed cultivation has proved a successful means to produce agricultural crops in various wetland areas of the world. In freshwater lakes and wetlands, vegetables, flowers, and seedlings are grown in Bangladesh using this floating cultivation technique, without any additional irrigation or chemical fertiliser. No detailed study of this indigenous cultivation technique has been published to date, although the laboratory method, hydroponics, is well documented in the professional literatu...

  17. Network Surrounding and Cultivation of Citizenship Consciousness——Based on the Theories of Deliberative Democracy%网络围观与公民意识培育——基于“审议民主”的视角

    Institute of Scientific and Technical Information of China (English)

    李艳

    2012-01-01

    As an emerging political participation way in the information age, network surrounding plays a positive role in cultivation of citizenship consciousness. But the virtual characteristic and case features of network surrounding cause lacking in discipline constrain of interact users' statements and actions and losing persistent power of cultivation of citizenship conscious- ness. Based on theories of Deliberative Democracy, there are three ways to eliminate adverse impact of network surrounding on the cultivation of citizenship consciousness. Firstly, "deliberative" should be encouraged in "Weak Public" to construct an ideal public expression space; Secondly, a communication platform should be built between "Strong Publics" and "Weak Publics" as to. absorb public opinions; Thirdly, "Strong Publics" should emphasize legitimacy and clarifying.

  18. 基于DEA模型的山东省耕地投入产出效率研究%RESEARCH ON INPUT-OUTPUT EFFICIENCY OF CULTIVATED LAND IN SHANDONG PROVINCE BASED ON DEA

    Institute of Scientific and Technical Information of China (English)

    宫攀; 韩振铃

    2015-01-01

    Cultivated land recourses was the most important natural resources, which was the basic guarantee of food security. Shandong province was one of China's most important bases of commodity grain production. In 2010, the arable land in Shandong was 7 , 510 , 761 hectares which accounting for 64. 95%of agricultural land and 47. 8%of the land area. The total output of Shandong in 2010 was 43. 53 million tons of grain, accounting for 7. 97%of total output in China. Firstly,this paper constructed the input and output index system with the input and output of cultivated land in Shandong province combined with availability of data and the actual situation. This index system divided into in-puts,outputs two levels. The output index selected economic efficiency and the social efficiency. The economic effi-ciency index included the output value of planting industry which reflected the scale of planting in study area,and value added of farming which expressed operation activity and service achievement. The social efficiency index re-flected by the grain output which mainly considerate that the social benefits of the arable land was mainly to main-tain social stability,and ensure food security. For investment-type indicators selected agricultural production inputs, labor inputs and land inputs. Secondly,this paper analyzed the change trend of the data of Shan Dong province's 17 municipalities during 2001~2010. Thirdly,the paper calculated the cultivated land use efficiency by the DEA mod-el using the data of Shan Dong province in 11 years. Besides,it had investigated the influences of the scale and pure technical factor on cultivated land use efficiency in different areas. The result showed that the output indexes including the total agricultural output value and the grain output all presented general increasing trend. According to the input indexes,from 2000 to 2010, 8 municipalities' crop sown area decreased while 9 of them increased;All the regions have improved their

  19. A perceptual hashing method based on luminance features

    Science.gov (United States)

    Luo, Siqing

    2011-02-01

    With the rapid development of multimedia technology, content based searching and image authentication has become strong requirements. Image hashing technique has been proposed to meet them. In this paper, an RST (Rotation, Scaling, and Translation) resistant image hash algorithm is presented. In this method, the geometric distortions are extracted and adjusted by normalization. The features of the image are generated from the high-rank moments of luminance distribution. With the help of the efficient image representation capability of high-rank moments, the robustness and discrimination of proposed method are improved. The experimental results show that the proposed method is better than some existing methods in robustness under rotation attack.

  20. Color Restoration Method Based on Spectral Information Using Normalized Cut

    Institute of Scientific and Technical Information of China (English)

    Tetsuro Morimoto; Tohru Mihashi; Katsushi Ikeuchi

    2008-01-01

    This paper proposes a novel method for color restoration that can effectively apply accurate color based on spectral information to a segmented image using the normalized cut technique. Using the proposed method, we can obtain a digital still camera image and spectral information in different environments. Also, it is not necessary to estimate reflectance spectra using a spectral database such as other methods. The synthesized images are accurate and high resolution. The proposed method effectively works in making digital archive contents. Some experimental results are demonstrated in this paper.

  1. Analysis of fault detection method based on predictive filter approach

    Institute of Scientific and Technical Information of China (English)

    LI Ji; ZHANG Hongyue

    2005-01-01

    A new detection method for component faults based on predictive filters together with the fault detectability, false alarm rate, missed alarm rate and upper bound of detection time are proposed. The efficiency of the method is illustrated by a simulation example of a second-order system. It is shown that the fault detection method using predictive filters has a small delay, a low false alarm rate and a low missed alarm rate. Furthermore the filter can give accurate estimates of states even after a fault occurs. The real-time estimation provided by this method can also be used for fault tolerant control.

  2. Novel orchid varieties cultivated

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ A research team led by Prof.DUAN Jun from the CAS South China Botanical Garden has been successful in breeding new orchid hybrids. One of them,Haraenopsis Nanzhi Pink (coming from Phalaenopsis Wedding Promenade and Haraella retrocalla) has been accepted as a new hybrid genus by the London-based Royal Horticulture Society (RHS). It is the first new hybrid genus successfully registered at RHS by orchid breeders from Chinese mainland.

  3. A reservoir skeleton-based multiple point geostatistics method

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Traditional stochastic reservoir modeling,including object-based and pixel-based methods,cannot solve the problem of reproducing continuous and curvilinear reservoir objects. The paper first dives into the various stochastic modeling methods and extracts their merits,then proposes the skeleton-based multiple point geostatistics(SMPS) for the fluvial reservoir. The core idea is using the skeletons of reservoir objects to restrict the selection of data patterns. The skeleton-based multiple point geostatistics consists of two steps. First,predicting the channel skeleton(namely,channel centerline) by using the method in object-based modeling. The paper proposes a new method of search window to predict the skeleton. Then forecasting the distributions of reservoir objects using multiple point geostatistics with the restriction of channel skeleton. By the restriction of channel centerline,the selection of data events will be more reasonable and the realization will be achieved more really. The checks by the conceptual model and the real reservoir show that SMPS is much better than Sisim(sequential indicator simulation) ,Snesim(Single Normal Equation Simulation) and Simpat(simulation with patterns) in building the fluvial reservoir model. This new method will contribute to both the theoretical research of stochastic modeling and the oilfield developments of constructing highly precise reservoir geological models.

  4. a Minimum Spanning Tree Based Method for Uav Image Segmentation

    Science.gov (United States)

    Wang, Ping; Wei, Zheng; Cui, Weihong; Lin, Zhiyong

    2016-06-01

    This paper proposes a Minimum Span Tree (MST) based image segmentation method for UAV images in coastal area. An edge weight based optimal criterion (merging predicate) is defined, which based on statistical learning theory (SLT). And we used a scale control parameter to control the segmentation scale. Experiments based on the high resolution UAV images in coastal area show that the proposed merging predicate can keep the integrity of the objects and prevent results from over segmentation. The segmentation results proves its efficiency in segmenting the rich texture images with good boundary of objects.

  5. NOVEL RADAR SIGNAL SORTING METHOD BASED ON GEOMETRIC COVERING

    Institute of Scientific and Technical Information of China (English)

    万建; 国强; 宋文明

    2013-01-01

    With the increase of complexity of electromagnetic environment and continuous appearance of advanced system radars ,signals received by radar reconnaissance receivers become even more intensive and complex .There-fore ,traditional radar sorting methods based on neural network algorithms and support vector machine (SVM ) cannot process them effectively .Aiming at solving this problem ,a novel radar signal sorting method based on the cloud model theory and the geometric covering algorithm is proposed .By applying the geometric covering algo-rithm to divide input signals into different covering domains based on their distribution characteristics ,the method can overcome a typical problem that it is easy for traditional sorting algorithms to fall into the local extrema due to the use of complex nonlinear equation to describe input signals .The method uses the cloud model to describe the membership degree between signals to be sorted and their covering domains ,thus it avoids the disadvantage that traditional sorting methods based on hard clustering cannot deinterleave the signal samples with overlapped param-eters .Experimental results show that the presented method can effectively sort advanced system radar signals with overlapped parameters in complex electromagnetic environment .

  6. Cultivating Students' English Autonomous Learning Ability in Web-based Environment%网络环境下大学生英语自主学习能力培养

    Institute of Scientific and Technical Information of China (English)

    张兴华; 杨洪娟

    2014-01-01

    该文分析网络环境下大学生英语自主学习存在的问题,并从网络环境下教师角色转变、课堂活动开展、校园网络资源平台建设、学生自主学习能力培训和指导、学生自主学习过程监控、评价、激励等机制指出如何培养网络环境下大学生英语自主学习能力。%This paper analyzes the problems of college students' autonomous learning in the web-based environment and points out how to cultivate students' English autonomous learning ability in the web-based environment from the changing role of teachers in the web-based environment, the launching of classroom activities, the construction of the campus network resourc-es platform, the training and guidance of the students' autonomous learning ability, autonomous learning process monitoring, evaluation, incentives and other mechanisms.

  7. 《组织行为学》案例教学与学生创新能力的培养%Organizational Behavior Case-based Teaching and cultivation of students' innovative ability

    Institute of Scientific and Technical Information of China (English)

    钟曼丽

    2011-01-01

    Cultivation of innovative ability is one of the main tasks of case - based teaching.This paper combines oneself engaged in management course of the case - base teaching practice,this paper discusses the organizational behavior case - based teaching throug%创新能力的培养是案例教学的主要任务之一。本文结合自己从事管理类课程案例教学的实践,探讨《组织行为学》案例教学中通过案例教学课程设计创新意识培养、教学组织创建新环境、和教学方法培养创新思维的各种途径及方法,对改善《组织行为学》课程案例教学、提高学生的创新能力提出了新的思路。

  8. A flower image retrieval method based on ROI feature

    Institute of Scientific and Technical Information of China (English)

    洪安祥; 陈刚; 李均利; 池哲儒; 张亶

    2004-01-01

    Flower image retrieval is a very important step for computer-aided plant species recognition.In this paper,we propose an efficient segmentation method based on color clustering and domain knowledge to extract flower regions from flower images.For flower retrieval,we use the color histogram of a flower region to characterize the color features of flower and two shape-based features sets,Centroid-Contour Distance(CCD)and Angle Code Histogram(ACH),to characterize the shape features of a flower contour.Experimental results showed that our flower region extraction method based on color clustering and domain knowledge can produce accurate flower regions.Flower retrieval results on a database of 885 flower images collected from 14 plant species showed that our Region-of-Interest(ROD based retrieval approach using both color and shape features can perform better than a method based on the global color histogram proposed by Swain and Ballard(1991)and a method based on domain knowledge-driven segmentation and color names proposed by Das et al.(1999).

  9. A flower image retrieval method based on ROI feature

    Institute of Scientific and Technical Information of China (English)

    洪安祥; 陈刚; 李均利; 池哲儒; 张亶

    2004-01-01

    Flower image retrieval is a very important step for computer-aided plant species recognition. In this paper, we propose an efficient segmentation method based on color clustering and domain knowledge to extract flower regions from flower images. For flower retrieval, we use the color histogram of a flower region to characterize the color features of flower and two shape-based features sets, Centroid-Contour Distance (CCD) and Angle Code Histogram (ACH), to characterize the shape features of a flower contour. Experimental results showed that our flower region extraction method based on color clustering and domain knowledge can produce accurate flower regions. Flower retrieval results on a database of 885 flower images collected from 14 plant species showed that our Region-of-Interest (ROI) based retrieval approach using both color and shape features can perform better than a method based on the global color histogram proposed by Swain and Ballard (1991) and a method based on domain knowledge-driven segmentation and color names proposed by Das et al.(1999).

  10. Facial Feature Extraction Method Based on Coefficients of Variances

    Institute of Scientific and Technical Information of China (English)

    Feng-Xi Song; David Zhang; Cai-Kou Chen; Jing-Yu Yang

    2007-01-01

    Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) are two popular feature ex- traction techniques in statistical pattern recognition field. Due to small sample size problem LDA cannot be directly applied to appearance-based face recognition tasks. As a consequence, a lot of LDA-based facial feature extraction techniques are proposed to deal with the problem one after the other. Nullspace Method is one of the most effective methods among them. The Nullspace Method tries to find a set of discriminant vectors which maximize the between-class scatter in the null space of the within-class scatter matrix. The calculation of its discriminant vectors will involve performing singular value decomposition on a high-dimensional matrix. It is generally memory- and time-consuming. Borrowing the key idea in Nullspace method and the concept of coefficient of variance in statistical analysis we present a novel facial feature extraction method, i.e., Discriminant based on Coefficient of Variance (DCV) in this paper. Experimental results performed on the FERET and AR face image databases demonstrate that DCV is a promising technique in comparison with Eigenfaces, Nullspace Method, and other state-of-the-art facial feature extraction methods.

  11. Quaternion-based discriminant analysis method for color face recognition.

    Science.gov (United States)

    Xu, Yong

    2012-01-01

    Pattern recognition techniques have been used to automatically recognize the objects, personal identities, predict the function of protein, the category of the cancer, identify lesion, perform product inspection, and so on. In this paper we propose a novel quaternion-based discriminant method. This method represents and classifies color images in a simple and mathematically tractable way. The proposed method is suitable for a large variety of real-world applications such as color face recognition and classification of the ground target shown in multispectrum remote images. This method first uses the quaternion number to denote the pixel in the color image and exploits a quaternion vector to represent the color image. This method then uses the linear discriminant analysis algorithm to transform the quaternion vector into a lower-dimensional quaternion vector and classifies it in this space. The experimental results show that the proposed method can obtain a very high accuracy for color face recognition. PMID:22937054

  12. Testability integrated evaluation method based on testability virtual test data

    Directory of Open Access Journals (Sweden)

    Liu Guanjun

    2014-02-01

    Full Text Available Testability virtual test is a new test method for testability verification, which has the advantages such as low cost, few restrictions and large sample of test data. It can be used to make up the deficiency of testability physical test. In order to take the advantage of testability virtual test data effectively and to improve the accuracy of testability evaluation, a testability integrated evaluation method is proposed in this paper based on testability virtual test data. Considering the characteristic of testability virtual test data, the credibility analysis method for testability virtual test data is studied firstly. Then the integrated calculation method is proposed fusing the testability virtual and physical test data. Finally, certain helicopter heading and attitude system is presented to demonstrate the proposed method. The results show that the testability integrated evaluation method is feasible and effective.

  13. Testability integrated evaluation method based on testability virtual test data

    Institute of Scientific and Technical Information of China (English)

    Liu Guanjun; Zhao Chenxu; Qiu Jing; Zhang Yong

    2014-01-01

    Testability virtual test is a new test method for testability verification, which has the advantages such as low cost, few restrictions and large sample of test data. It can be used to make up the deficiency of testability physical test. In order to take the advantage of testability virtual test data effectively and to improve the accuracy of testability evaluation, a testability integrated eval-uation method is proposed in this paper based on testability virtual test data. Considering the char-acteristic of testability virtual test data, the credibility analysis method for testability virtual test data is studied firstly. Then the integrated calculation method is proposed fusing the testability vir-tual and physical test data. Finally, certain helicopter heading and attitude system is presented to demonstrate the proposed method. The results show that the testability integrated evaluation method is feasible and effective.

  14. Preconditioned WR-LMF-based method for ODE systems

    Science.gov (United States)

    Jin, Xiao-Qing; Sin, Vai-Kuong; Song, Li-Li

    2004-01-01

    The waveform relaxation (WR) method was developed as an iterative method for solving large systems of ordinary differential equations (ODEs). In each WR iteration, we are required to solve a system of ODEs. We then introduce the boundary value method (BVM) which is a relatively new method based on the linear multistep formulae to solve ODEs. In particular, we apply the generalized minimal residual method with the Strang-type block-circulant preconditioner for solving linear systems arising from the application of BVMs to each WR iteration. It is demonstrated that these techniques are very effective in speeding up the convergence rate of the resulting iterative processes. Numerical experiments are presented to illustrate the effectiveness of our methods.

  15. A hybrid method for pancreas extraction from CT image based on level set methods.

    Science.gov (United States)

    Jiang, Huiyan; Tan, Hanqing; Fujita, Hiroshi

    2013-01-01

    This paper proposes a novel semiautomatic method to extract the pancreas from abdominal CT images. Traditional level set and region growing methods that request locating initial contour near the final boundary of object have problem of leakage to nearby tissues of pancreas region. The proposed method consists of a customized fast-marching level set method which generates an optimal initial pancreas region to solve the problem that the level set method is sensitive to the initial contour location and a modified distance regularized level set method which extracts accurate pancreas. The novelty in our method is the proper selection and combination of level set methods, furthermore an energy-decrement algorithm and an energy-tune algorithm are proposed to reduce the negative impact of bonding force caused by connected tissue whose intensity is similar with pancreas. As a result, our method overcomes the shortages of oversegmentation at weak boundary and can accurately extract pancreas from CT images. The proposed method is compared to other five state-of-the-art medical image segmentation methods based on a CT image dataset which contains abdominal images from 10 patients. The evaluated results demonstrate that our method outperforms other methods by achieving higher accuracy and making less false segmentation in pancreas extraction.

  16. A SUBARRAY-SYNTHESIS BASED 2D DOA ESTIMATION METHOD

    Institute of Scientific and Technical Information of China (English)

    Xu Wenlong; Jiang Wei; Li Zengfu; Shang Yong; Xiang Haige

    2006-01-01

    In some satellite communications, we need to perform Direction Of Arrival (DOA) angle estimation under the restriction that the number of receivers is less than that of the array elements in an array antenna.To solve the conundrum, a method named subarray-synthesis-based Two-Dimensional DOA (2D DOA) angle estimation is proposed. In the method, firstly, the array antenna is divided into a series of subarray antennas based on the total number of receivers; secondly, the subarray antennas' output covariance matrices are estimated; thirdly, an equivalent covariance matrix is synthesized based on the subarray output covariance matrices; then 2D DOA estimation is performed. Monte Carlo simulations showed that the estimation method is effective.

  17. International Conference on Robust Rank-Based and Nonparametric Methods

    CERN Document Server

    McKean, Joseph

    2016-01-01

    The contributors to this volume include many of the distinguished researchers in this area. Many of these scholars have collaborated with Joseph McKean to develop underlying theory for these methods, obtain small sample corrections, and develop efficient algorithms for their computation. The papers cover the scope of the area, including robust nonparametric rank-based procedures through Bayesian and big data rank-based analyses. Areas of application include biostatistics and spatial areas. Over the last 30 years, robust rank-based and nonparametric methods have developed considerably. These procedures generalize traditional Wilcoxon-type methods for one- and two-sample location problems. Research into these procedures has culminated in complete analyses for many of the models used in practice including linear, generalized linear, mixed, and nonlinear models. Settings are both multivariate and univariate. With the development of R packages in these areas, computation of these procedures is easily shared with r...

  18. On the Cultivation of Intensive Reading Skills

    Institute of Scientific and Technical Information of China (English)

    杨雪琴

    2008-01-01

    This paper concerns the importance of the cultivation of reading skills,the characteristics of intensive reading and how to cultivate the intensive reading skills.Since the focus of this papre is how to cultivate the intensive reading skills,we make a systematic exposition from three points: word study,grammar patterns and text.

  19. Image mosaic method based on SIFT features of line segment.

    Science.gov (United States)

    Zhu, Jun; Ren, Mingwu

    2014-01-01

    This paper proposes a novel image mosaic method based on SIFT (Scale Invariant Feature Transform) feature of line segment, aiming to resolve incident scaling, rotation, changes in lighting condition, and so on between two images in the panoramic image mosaic process. This method firstly uses Harris corner detection operator to detect key points. Secondly, it constructs directed line segments, describes them with SIFT feature, and matches those directed segments to acquire rough point matching. Finally, Ransac method is used to eliminate wrong pairs in order to accomplish image mosaic. The results from experiment based on four pairs of images show that our method has strong robustness for resolution, lighting, rotation, and scaling. PMID:24511326

  20. Image Mosaic Method Based on SIFT Features of Line Segment

    Directory of Open Access Journals (Sweden)

    Jun Zhu

    2014-01-01

    Full Text Available This paper proposes a novel image mosaic method based on SIFT (Scale Invariant Feature Transform feature of line segment, aiming to resolve incident scaling, rotation, changes in lighting condition, and so on between two images in the panoramic image mosaic process. This method firstly uses Harris corner detection operator to detect key points. Secondly, it constructs directed line segments, describes them with SIFT feature, and matches those directed segments to acquire rough point matching. Finally, Ransac method is used to eliminate wrong pairs in order to accomplish image mosaic. The results from experiment based on four pairs of images show that our method has strong robustness for resolution, lighting, rotation, and scaling.

  1. Analysis of Dynamic Modeling Method Based on Boundary Element

    Directory of Open Access Journals (Sweden)

    Xu-Sheng Gan

    2013-07-01

    Full Text Available The aim of this study was to study an improved dynamic modeling method based on a Boundary Element Method (BEM. The dynamic model was composed of the elements such as the beam element, plate element, joint element, lumped mass and spring element by the BEM. An improved dynamic model of a machine structure was established based on plate-beam element system mainly. As a result, the dynamic characteristics of a machine structure were analyzed and the comparison of computational results and experimental’s showed the modeling method was effective. The analyses indicate that the introduced method inaugurates a good way for analyzing dynamic characteristics of a machine structure efficiently.

  2. A Triangle Mesh Standardization Method Based on Particle Swarm Optimization.

    Science.gov (United States)

    Wang, Wuli; Duan, Liming; Bai, Yang; Wang, Haoyu; Shao, Hui; Zhong, Siyang

    2016-01-01

    To enhance the triangle quality of a reconstructed triangle mesh, a novel triangle mesh standardization method based on particle swarm optimization (PSO) is proposed. First, each vertex of the mesh and its first order vertices are fitted to a cubic curve surface by using least square method. Additionally, based on the condition that the local fitted surface is the searching region of PSO and the best average quality of the local triangles is the goal, the vertex position of the mesh is regulated. Finally, the threshold of the normal angle between the original vertex and regulated vertex is used to determine whether the vertex needs to be adjusted to preserve the detailed features of the mesh. Compared with existing methods, experimental results show that the proposed method can effectively improve the triangle quality of the mesh while preserving the geometric features and details of the original mesh. PMID:27509129

  3. Nearfield acoustic holography based on the equivalent source method

    Institute of Scientific and Technical Information of China (English)

    BI Chuanxing; CHEN Xinzhao; CHEN Jian; ZHOU Rong

    2005-01-01

    On the basis of the nearfield acoustic holography (NAH) based on the distributed source boundary point method (DSBPM), a novel NAH based on the equivalent source method (ESM) is proposed. The theoretical model of the proposed method is established at first. And then, the error sensitivity and the reconstruction problems of a multi-source acoustic field and a semi-free acoustic field are analyzed, and the corresponding treatment methods are proposed. Subsequently, an experiment on a speaker is investigated to validate the feasibility and correctness of the method. In the method, no discretization is needed on the boundary of the vibrating body. The acoustic field is predicted directly by a set of weighted equivalent sources located inside the vibrating body. Therefore, the variable interpolation, the treatments of singular integrals and non-uniqueness of solutions in the characteristic wave number are avoided. Additionally, the method is adapted to arbitrary-shaped source, so it is really a meshless method. Furthermore, there are such merits as the simple principle, the high calculation efficiency and precision. It is valuabe for the NAH to be applied in the practical engineering field.

  4. Local coding based matching kernel method for image classification.

    Directory of Open Access Journals (Sweden)

    Yan Song

    Full Text Available This paper mainly focuses on how to effectively and efficiently measure visual similarity for local feature based representation. Among existing methods, metrics based on Bag of Visual Word (BoV techniques are efficient and conceptually simple, at the expense of effectiveness. By contrast, kernel based metrics are more effective, but at the cost of greater computational complexity and increased storage requirements. We show that a unified visual matching framework can be developed to encompass both BoV and kernel based metrics, in which local kernel plays an important role between feature pairs or between features and their reconstruction. Generally, local kernels are defined using Euclidean distance or its derivatives, based either explicitly or implicitly on an assumption of Gaussian noise. However, local features such as SIFT and HoG often follow a heavy-tailed distribution which tends to undermine the motivation behind Euclidean metrics. Motivated by recent advances in feature coding techniques, a novel efficient local coding based matching kernel (LCMK method is proposed. This exploits the manifold structures in Hilbert space derived from local kernels. The proposed method combines advantages of both BoV and kernel based metrics, and achieves a linear computational complexity. This enables efficient and scalable visual matching to be performed on large scale image sets. To evaluate the effectiveness of the proposed LCMK method, we conduct extensive experiments with widely used benchmark datasets, including 15-Scenes, Caltech101/256, PASCAL VOC 2007 and 2011 datasets. Experimental results confirm the effectiveness of the relatively efficient LCMK method.

  5. XML-based product information processing method for product design

    Science.gov (United States)

    Zhang, Zhen Yu

    2012-01-01

    Design knowledge of modern mechatronics product is based on information processing as the center of the knowledge-intensive engineering, thus product design innovation is essentially the knowledge and information processing innovation. Analysis of the role of mechatronics product design knowledge and information management features, a unified model of XML-based product information processing method is proposed. Information processing model of product design includes functional knowledge, structural knowledge and their relationships. For the expression of product function element, product structure element, product mapping relationship between function and structure based on the XML model are proposed. The information processing of a parallel friction roller is given as an example, which demonstrates that this method is obviously helpful for knowledge-based design system and product innovation.

  6. CONSTRUCTION METHOD OF KNOWLEDGE MAP BASED ON DESIGN PROCESS

    Institute of Scientific and Technical Information of China (English)

    SU Hai; JIANG Zuhua

    2007-01-01

    Due to the increasing amount and complexity of knowledge in product design, the knowledge map based on design process is presented as a tool to reuse product design process, promote the product design knowledge sharing. The relationship between design task flow and knowledge flow is discussed; A knowledge organizing method based on design task decomposition and a visualization method to support the knowledge retrieving and sharing in product design are proposed. And a knowledge map system to manage the knowledge in product design process is built with Visual C++ and SVG. Finally, a brief case study is provided to illustrate the construction and application of knowledge map in fuel pump design.

  7. Dynamic Filtering Method for GPS Based on Multi-Scale

    Directory of Open Access Journals (Sweden)

    Tingjun Li

    2013-01-01

    Full Text Available Aiming at GPS dynamic filtering method, this paper uses the method of multi-scale analysis, combines the “current” statistical model of automotive carrier with multi-scale signal transformation which is based on statistical characteristic, establishes the new algorithm of multi-scale data fusion for GPS dynamic filter combining with normal Kalman filter algorithm, at last achieves the optimal fusion estimated value of the target states based on global information at the finest scale. When the above algorithm is used to GPS dynamic filter, the simulated results show that the proposed algorithm can effectively increase estimated precision of target states compared with the conventional KF.

  8. An Image Encryption Method Based on Bit Plane Hiding Technology

    Institute of Scientific and Technical Information of China (English)

    LIU Bin; LI Zhitang; TU Hao

    2006-01-01

    A novel image hiding method based on the correlation analysis of bit plane is described in this paper. Firstly, based on the correlation analysis, different bit plane of a secret image is hided in different bit plane of several different open images. And then a new hiding image is acquired by a nesting "Exclusive-OR" operation on those images obtained from the first step. At last, by employing image fusion technique, the final hiding result is achieved. The experimental result shows that the method proposed in this paper is effective.

  9. A Novel Spectrum Handoff Method Based On Spectrum Reservation

    Directory of Open Access Journals (Sweden)

    Zheng Yong

    2013-07-01

    Full Text Available In this paper, we apply fuzzy analytic hierarchy process (FAHP method in the decision process of Cognitive Radio Networks (CRN spectrum handoff. Based on the pre-determined target spectrum list model, considering the multiple indicators which influence the handoff performance, we designed a spectrum handoff method based on spectrum reservation strategy. Simulation results show that the algorithm proposed in this paper exceeds the random handoff algorithm without channel order, it can significantly reduce the handoff frequency and time overhead of cognitive users, reduce the system delay and improve the system throughput.

  10. A Robust Digital Watermark Extracting Method Based on Neural Network

    Institute of Scientific and Technical Information of China (English)

    GUOLihua; YANGShutang; LIJianhua

    2003-01-01

    Since watermark removal software, such as StirMark, has succeeded in washing watermarks away for most of the known watermarking systems, it is necessary to improve the robustness of watermarking systems. A watermark extracting method based on the error Back propagation (BP) neural network is presented in this paper, which can efficiently improve the robustness of watermarking systems. Experiments show that even if the watermarking systems are attacked by the StirMark software, the extracting method based on neural network can still efficiently extract the whole watermark information.

  11. Method of infrared image enhancement based on histogram

    Institute of Scientific and Technical Information of China (English)

    WANG Liang; YAN Jie

    2011-01-01

    Aiming at the problem in infrared image enhancement, a new method is given based on histogram. Using the gray character- istics of target, the upper-bouod threshold is selected adaptively and the histogram is processed by the threshold. After choosing the gray transform function based on the gray level distribution of image, the gray transformation is done during histogram equalization. Finally, the enhanced image is obtained. Compared with histogram equalization (HE), histogram double equalization (HDE) and plateau histogram equalization (PE), the simulation results demonstrate that the image enhancement effect of this method has obvious superiority. At the same time, its operation speed is fast and real-time ability is excellent.

  12. Harbourscape Aalborg - Design Based Methods in Waterfront Development

    DEFF Research Database (Denmark)

    Kiib, Hans

    2012-01-01

    and Performative Architecture Workshop in 2008, and evaluates the method and the thinking behind this. The design workshops provide different design-based development methods which can be tested with the purpose of developing new concepts for the relationship between the city and its harbour, and in addition...... an independent contribution to the visioning process on urban development, city life planning and landscaping, but this has to be based on a “non-dogmatic” approach in the architectural and urban space design. This involves, among other aspects, the combination of independent evaluation and discourse analyses...

  13. Wavelet basis construction method based on separation blast vibration signal

    Institute of Scientific and Technical Information of China (English)

    凌同华; 张胜; 陈倩倩; 李洁

    2015-01-01

    As wavelet basis in wavelet analysis is neither arbitrary nor unique, the same signal dealing with different wavelet bases will generate different results. Therefore, how to construct a wavelet basis suitable for the characteristics of the analyzed signal and solve its algorithm and realization is a fundamental problem which perplexed many researchers. To solve these problems, in accordance with the basic features of the measured millisecond blast vibration signal, a new wavelet basis construction method based on the separation blast vibration signal is proposed, and the feasibility of this method is verified by comparing the practical effect of the newly constructed wavelet with other known wavelets in signal processing.

  14. IDEF method-based simulation model design and development framework

    Directory of Open Access Journals (Sweden)

    Ki-Young Jeong

    2009-09-01

    Full Text Available The purpose of this study is to provide an IDEF method-based integrated framework for a business process simulation model to reduce the model development time by increasing the communication and knowledge reusability during a simulation project. In this framework, simulation requirements are collected by a function modeling method (IDEF0 and a process modeling method (IDEF3. Based on these requirements, a common data model is constructed using the IDEF1X method. From this reusable data model, multiple simulation models are automatically generated using a database-driven simulation model development approach. The framework is claimed to help both requirement collection and experimentation phases during a simulation project by improving system knowledge, model reusability, and maintainability through the systematic use of three descriptive IDEF methods and the features of the relational database technologies. A complex semiconductor fabrication case study was used as a testbed to evaluate and illustrate the concepts and the framework. Two different simulation software products were used to develop and control the semiconductor model from the same knowledge base. The case study empirically showed that this framework could help improve the simulation project processes by using IDEF-based descriptive models and the relational database technology. Authors also concluded that this framework could be easily applied to other analytical model generation by separating the logic from the data.

  15. Hybrid Fundamental Solution Based Finite Element Method: Theory and Applications

    OpenAIRE

    Changyong Cao; Qing-Hua Qin

    2015-01-01

    An overview on the development of hybrid fundamental solution based finite element method (HFS-FEM) and its application in engineering problems is presented in this paper. The framework and formulations of HFS-FEM for potential problem, plane elasticity, three-dimensional elasticity, thermoelasticity, anisotropic elasticity, and plane piezoelectricity are presented. In this method, two independent assumed fields (intraelement filed and auxiliary frame field) are employed. The formulations for...

  16. A questionnaire based evaluation of teaching methods amongst MBBS students

    OpenAIRE

    Muneshwar JN, Mirza Shiraz Baig, Zingade US, Khan ST

    2013-01-01

    The medical education and health care in India are facing serious challenges in content and competencies. Heightened focus on the quality of teaching in medical college has led to increased use of student surveys as a means of evaluating teaching. Objectives: A questionnaire based evaluation of 200 students (I MBBS & II MBBS) about teaching methods was conducted at a Govt Medical College & Hospital, Aurangabad (MS) with intake capacity of 150 students &established since 50 last years. Methods...

  17. NUMERICAL METHODS FOR DIFFERENTIAL GAMES BASED ON PARTIAL DIFFERENTIAL EQUATIONS

    OpenAIRE

    Falcone, M

    2006-01-01

    In this paper we present some numerical methods for the solution of two-persons zero-sum deterministic differential games. The methods are based on the dynamic programming approach. We first solve the Isaacs equation associated to the game to get an approximate value function and then we use it to reconstruct approximate optimal feedback controls and optimal trajectories. The approximation schemes also have an interesting control interpretation since the time-discrete scheme stems from a dyna...

  18. Progress of DNA-based Methods for Species Identification

    Institute of Scientific and Technical Information of China (English)

    HU Zhen; ZHANG Su-hua; WANG Zheng; BIAN Ying-nan; LI Cheng-tao

    2015-01-01

    Species identification of biological samples is widely used in such fields as forensic science and food industry. A variety of accurate and reliable methods have been developed in recent years. The cur-rent reviewshows common target genes and screening criteria suitable for species identification, and de-scribed various DNA-based molecular biology methods about species identification. Additionally, it dis-cusses the future development of species identification combined with real-time PCR and sequencing technologies.

  19. New de-interlacing method based on adaptive weight

    Institute of Scientific and Technical Information of China (English)

    赵建伟; 古雪丰; 王朋; 刘重庆

    2004-01-01

    De-interlacing is very important when converting interlaced pictures to progressive pictures in format conversion.Multi-formats digital broadcast and progressive display requires the de-interlacing technique. An adaptive weight deinterlacing method is proposed. It combines motion compensation technique with directional-based spatio-temporal filter efficiently. Experiment results indicate that the method can keep edge continuity and sharpness effectively, reduce the artifacts in motion areas, and shows better visual performance when the estimated motion vectors are inaccurate.

  20. Cultivated Characters of Nandina domestica%南天竹栽培性状研究

    Institute of Scientific and Technical Information of China (English)

    李湉

    2009-01-01

    [Objective] This study was to systematically investigate the cultivated characters of Nandina domestica and to lay a foundation for breeding, cultivating, as well as its application in landscape. [Method] Using N.domestica populations from 13 different areas of Hunan Province as tested samples, the cultivated characters of N.domestica in both the cultivated type and wild type were systematically studied through field experiment and comparative analysis. [Resul] The phenological features of N.domestica from different areas and germplasm resources were basically identical; the growth patterns of cultivated type and wild type were also basically identical, but the cultivated type grew faster than the wild type; both the two types grew quickly at young stage, and either the annual growth of new shoots or the growth pattern of leaves confirmed the fitting curve of the Logistic equation. [Conclusion] This research provided basis for the exploitation and utilization the germplasm resources of N.domestica.