WorldWideScience

Sample records for based cultivation method

  1. Diversity surveys of soil bacterial community by cultivation-based methods and molecular fingerprinting techniques

    Institute of Scientific and Technical Information of China (English)

    LUO Hai-feng; QI Hong-yan; ZHANG Hong-xun

    2004-01-01

    By combining the cultivation methods with molecular fingerprinting techniques, the diversity surveys of soil bacterial community in 13 areas of China were carried out. The cultivable heterotrophic diversity was investigated by colony morphology on solid LB medium. Genetic diversity was measured as bands on denaturing gradient gel electrophoresis(DGGE) by the extraction and purification of the total soil DNA, and amplification of bacterial 16S rDNA fragments by polymerase chain reaction ( PCR). The Shannon-Wiener indices of diversity (H), richness (S)and evenness( EH ) were employed to estimate the diversity of soil bacterial community. The results showed that there was an obvious diversification existed in soil from the different areas. However, the genetic diversity estimated by PCR-DGGE can provide more comprehensive information on bacterial community than the cultivation-based methods. Therefore, it is suggested to combine the traditional methods with genetic fingerprinting techniques to survey and estimate soil bacterial diversity.

  2. [Dendrobium officinale stereoscopic cultivation method].

    Science.gov (United States)

    Si, Jin-Ping; Dong, Hong-Xiu; Liao, Xin-Yan; Zhu, Yu-Qiu; Li, Hui

    2014-12-01

    The study is aimed to make the most of available space of Dendrobium officinale cultivation facility, reveal the yield and functional components variation of stereoscopic cultivated D. officinale, and improve quality, yield and efficiency. The agronomic traits and yield variation of stereoscopic cultivated D. officinale were studied by operating field experiment. The content of polysaccharide and extractum were determined by using phenol-sulfuric acid method and 2010 edition of "Chinese Pharmacopoeia" Appendix X A. The results showed that the land utilization of stereoscopic cultivated D. officinale increased 2.74 times, the stems, leaves and their total fresh or dry weight in unit area of stereoscopic cultivated D. officinale were all heavier than those of the ground cultivated ones. There was no significant difference in polysaccharide content between stereoscopic cultivation and ground cultivation. But the extractum content and total content of polysaccharide and extractum were significantly higher than those of the ground cultivated ones. In additional, the polysaccharide content and total content of polysaccharide and extractum from the top two levels of stereoscopic culture matrix were significantly higher than that of the ones from the other levels and ground cultivation. Steroscopic cultivation can effectively improves the utilization of space and yield, while the total content of polysaccharides and extractum were significantly higher than that of the ground cultivated ones. The significant difference in Dendrobium polysaccharides among the plants from different height of stereo- scopic culture matrix may be associated with light factor.

  3. Assessment of Cultivation Method for Energy Beet Based on LCA Method

    Institute of Scientific and Technical Information of China (English)

    Chunfeng; ZHANG; Feng; LIU; Yuangang; ZU; Qingying; MENG; Baoguo; ZHU; Nannan; WANG

    2014-01-01

    In order to establish a supply system for energy resource coupled with the environment,the production technology of sugar beets was explored as a biological energy source. The low-humic andosol as the experimental soil,the panting method was direct planting,and cultivation technique was minimum tillage direct planting method. The control was conventional tillage transplant and no tillage direct planting. The results demonstrated that data revealed that the energy cost of no tillage and a direct planting method was 105 GJ/hm2on average for two years,while that of the conventional tillage method was 112 GJ/hm2per year. The ratio of output to input showed that the direct planting with no tillage was more efficient( 3. 61) than the conventional tillage( 3. 01). Moreover,the emission of CO2into the atmosphere with no tillage and the direct planting was 71% of the conventional tillage planting technique. Therefore,direct planting without tillage reduces the impact on the environment.

  4. Advanced continuous cultivation methods for systems microbiology.

    Science.gov (United States)

    Adamberg, Kaarel; Valgepea, Kaspar; Vilu, Raivo

    2015-09-01

    Increasing the throughput of systems biology-based experimental characterization of in silico-designed strains has great potential for accelerating the development of cell factories. For this, analysis of metabolism in the steady state is essential as only this enables the unequivocal definition of the physiological state of cells, which is needed for the complete description and in silico reconstruction of their phenotypes. In this review, we show that for a systems microbiology approach, high-resolution characterization of metabolism in the steady state--growth space analysis (GSA)--can be achieved by using advanced continuous cultivation methods termed changestats. In changestats, an environmental parameter is continuously changed at a constant rate within one experiment whilst maintaining cells in the physiological steady state similar to chemostats. This increases the resolution and throughput of GSA compared with chemostats, and, moreover, enables following of the dynamics of metabolism and detection of metabolic switch-points and optimal growth conditions. We also describe the concept, challenge and necessary criteria of the systematic analysis of steady-state metabolism. Finally, we propose that such systematic characterization of the steady-state growth space of cells using changestats has value not only for fundamental studies of metabolism, but also for systems biology-based metabolic engineering of cell factories.

  5. A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures

    Directory of Open Access Journals (Sweden)

    Glumoff Tuomo

    2010-02-01

    Full Text Available Abstract Background Cultivations for recombinant protein production in shake flasks should provide high cell densities, high protein productivity per cell and good protein quality. The methods described in laboratory handbooks often fail to reach these goals due to oxygen depletion, lack of pH control and the necessity to use low induction cell densities. In this article we describe the impact of a novel enzymatically controlled fed-batch cultivation technology on recombinant protein production in Escherichia coli in simple shaken cultures. Results The enzymatic glucose release system together with a well-balanced combination of mineral salts and complex medium additives provided high cell densities, high protein yields and a considerably improved proportion of soluble proteins in harvested cells. The cultivation method consists of three steps: 1 controlled growth by glucose-limited fed-batch to OD600 ~10, 2 addition of growth boosters together with an inducer providing efficient protein synthesis within a 3 to 6 hours period, and 3 a slow growth period (16 to 21 hours during which the recombinant protein is slowly synthesized and folded. Cell densities corresponding to 10 to 15 g l-1 cell dry weight could be achieved with the developed technique. In comparison to standard cultures in LB, Terrific Broth and mineral salt medium, we typically achieved over 10-fold higher volumetric yields of soluble recombinant proteins. Conclusions We have demonstrated that by applying the novel EnBase® Flo cultivation system in shaken cultures high cell densities can be obtained without impairing the productivity per cell. Especially the yield of soluble (correctly folded proteins was significantly improved in comparison to commonly used LB, Terrific Broth or mineral salt media. This improvement is thought to result from a well controlled physiological state during the whole process. The higher volumetric yields enable the use of lower culture volumes and can

  6. An isotope approach based on C-13 pulse-chase labelling vs. the root trenching method to separate heterotrophic and autotrophic respiration in cultivated peatlands

    Energy Technology Data Exchange (ETDEWEB)

    Biasi, C.; Pitkamaki, A. S.; Tavi, N. M.; Koponen, H. T.; Martikainen, P. J. [Univ.of Eastern Finland, Kuopio (Finland). Dept. of Environmental Science], e-mail: christina.biasi@uef.fi

    2012-11-01

    We tested an isotope method based on C-13 pulse-chase labelling for determining the fractional contribution of soil microbial respiration to overall soil respiration in an organic soil (cutaway peatland, eastern Finland), cultivated with the bioenergy crop, reed canary grass. The plants were exposed to CO{sub 2}-13 for five hours and the label was thereafter determined in CO{sub 2} derived from the soil-root system. A two-pool isotope mixing model was used to separate sources of respiration. The isotopic approach showed that a minimum of 50% of the total CO{sub 2} originated from soil-microbial respiration. Even though the method uses undisturbed soil-plant systems, it has limitations concerning the experimental determination of the true isotopic signal of all components contributing to autotrophic respiration. A trenching experiment which was comparatively conducted resulted in a 71% fractional contribution of soil-microbial respiration. This value was likely overestimated. Further studies are needed to evaluate critically the output from these two partitioning approaches. (orig.)

  7. Method of Cultivated Land Area Extraction Based on TM Data%基于TM数据耕地面积提取方法研究

    Institute of Scientific and Technical Information of China (English)

    王国芳

    2011-01-01

    粮食作物的播种面积、产量等信息是国家制定粮食政策和经济计划的重要依据.以昔阳县为研究区域,主要应用TM数据,结合研究区土地利用现状图,基于光谱信息,提取了耕地和非耕地信息,最后利用最大似然法监督分类得出耕地面积为29 242.1 hm2,分类结果精度达到93.3%.%Information of cultivation land area and cereal crops output is an important basis for the national food policy and the development of economic planning.In this paper, taking Xiyang as study case, TM data was used, land use map of the study area combined, and spectral information based, cultivated and non-cultivated land information was extracted.Finally, by maximum likelihood supervised classification, cultivation land area is 29 242.1 hm2 and its classification accuracy is as high as 93.3%.

  8. A Study on the Cultivation of College Students' Information Literacy Based on Task-driving Method%基于任务驱动法培养大学生信息素养的研究

    Institute of Scientific and Technical Information of China (English)

    丛荣华

    2011-01-01

    Through analyzing the significance of cultivating college students' information literacy and explaining the relation between information literacy and innovation ability training,this paper puts forward some strategies of cultivating college students' information literacy based on task-driving method.%本文通过对大学生信息素养培养意义的分析以及对信息素养与创新能力培养的关系的说明,详细论述了采用任务驱动法培养大学生信息素养的策略。

  9. Comparative Study of Rice Morphogenesis wit Different Cultivation Methods

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    [Objective] The aim of this study was to compare the morphological char- acteristics of rice (Oryza sativa L.) with different cultivation methods and investigate the dynamics of organ growth and development characteristics of different rice culti- vars. [Method] Based on continuous field observation and destructive sampling over a growing season, detailed organ morphological data were obtained including leaf length, node number, plant height, tiller number, leaf angle, leaf area and specific leaf weight, to compare organ morphological differences among 4 rice cultivars of Baidao (indica), Jinnanfeng (japonica), 9325 (japonica) and 9915 (japonica) with 3 cultivation methods of field planting in Weigang, pot planting in Weigang, and field planting in Jiangpu. [Result] Maximum leaf length of each node gradually increased at the early growth stage and decreased at the later growth stage, the relationship between maximum leaf length and node position can be described by the equations y=a,,-~ and y=ax+b; node number, growth duration, leaf length and plant height of pot planting rice in Weigang were smaller than that of the other two field planting meth- ods; the relationship between plant height and sunshine duration, plant height and GDD (growing degree days) can be described by the equation y=ax+b, 19.23 ℃.d of GDD (≥10 ≥-d) and 8.12 h of sunshine duration were required to increase 1 cm of plant height; plant height, tiller number, and leaf area of Baidao were higher than that of the other 3 laponica rice cultivars, but the specific leaf weight and leaf angle were smaller. [Conclusion] Comparison of morphological characteristic differ- ences among rice cultivars is an important way to select water-saving and drought- tolerant rice varieties. In this study, the experimental results can be integrated into a rice functional-architectural model to simulate rice organ growth dynamics in a three- dimensional space, thereby providing reference for selecting water

  10. Different cultivation methods to acclimatise ammonia-tolerant methanogenic consortia.

    Science.gov (United States)

    Tian, Hailin; Fotidis, Ioannis A; Mancini, Enrico; Angelidaki, Irini

    2017-02-11

    Bioaugmentation with ammonia tolerant-methanogenic consortia was proposed as a solution to overcome ammonia inhibition during anaerobic digestion process recently. However, appropriate technology to generate ammonia tolerant methanogenic consortia is still lacking. In this study, three basic reactors (i.e. batch, fed-batch and continuous stirred-tank reactors (CSTR)) operated at mesophilic (37°C) and thermophilic (55°C) conditions were assessed, based on methane production efficiency, incubation time, TAN/FAN (total ammonium nitrogen/free ammonia nitrogen) levels and maximum methanogenic activity. Overall, fed-batch cultivation was clearly the most efficient method compared to batch and CSTR. Specifically, by saving incubation time up to 150%, fed-batch reactors were acclimatised to nearly 2-fold higher FAN levels with a 37%-153% methanogenic activity improvement, compared to batch method. Meanwhile, CSTR reactors were inhibited at lower ammonia levels. Finally, specific methanogenic activity test showed that hydrogenotrophic methanogens were more active than aceticlastic methanogens in all FAN levels above 540mgNH3-NL(-1).

  11. 基于空间模拟退火算法的耕地质量布样及优化方法%Sampling and optimizing methods of cultivated land quality based on spatial simulated annealing algorithm

    Institute of Scientific and Technical Information of China (English)

    杨建宇; 岳彦利; 宋海荣; 汤赛; 叶思菁; 徐凡

    2015-01-01

    耕地质量监测是保障耕地资源的永续利用,实现耕地产能提升、加强耕地资源的管理、保护、合理利用的重要措施,对实现持续粮食安全具有重要意义.该文提出了基于空间模拟退火算法的耕地质量布样优化方法,以空间模拟退火算法为基础生成一组最优样本,构成基础监测网络,在此基础上,通过多期耕地等级成果数据提取属性发生变化的分等因素和对应发生变化的区域,生成潜在变化区,并结合研究区实际情况辅以专家知识和异常监测点,对基础样本点进行增加、删除、替换等优化操作,生成最终监测样点.以北京市大兴区为例,最终确定布设55个监测样点,结果表明,该方法布设的样点在耕地质量预测方面的精度高于传统的随机抽样和分层抽样方法,能有效地预测县域耕地质量并监控耕地质量的变化情况.%M Monitoring points in country area are the foundation to reflect changes of cultivated land quality, which directly affect the result of farmland grading and its accuracy. Through the monitoring network for cultivated land quality in county area, the distribution and changing trend of the cultivated land quality can be reflected. Besides, the quality of non-sampled locations should also be estimated with the data of sampling points. Due to the correlation among spatial samples, the traditional methods such as simple random sampling, stratified sampling and systematic sampling are inefficient to accomplish the task above. Thus, we propose a new spatial sampling and optimizing method based on the spatial simulated annealing (SSA). This paper presents a pre-processing method to determine the number of sampling points, including preprocessing the data of cultivated land quality before sampling, exploring the spatial correlation and spatial distribution pattern of cultivated land quality, and computing the appropriate quantity of sampling points by analyzing the

  12. Yunxian Walnut Cultivation Planning Method Based on ArcGIS%基于ArcGIS的云县核桃种植规划方法探讨

    Institute of Scientific and Technical Information of China (English)

    陈和彦

    2012-01-01

    It is necessary to consider the influence of soil, temperature , precipitation, elevation, slope, aspect and other site conditions on the growth, blossom, and resultant of trees for planting area planning. According to Yunnan walnut specific requirements of site conditions, combined with current situation of land utilization, followed the related law, based on ArcGIS software, this study fond out the suitable plan specific to the region by making classification evaluation, and plan map. Through area calculation, got suitable planting area of 4 556. 6hm , suitable cultivation area of 75 079. 65hm , planting area of 12 123. 46hm .%对某个树种适宜种植区域的规划要考虑到土壤、气温、降水量、海拔、坡度、坡向等立地条件对树木生长、开花、结果的影响.根据云南核桃对立地条件的特定要求,结合土地利用现状,遵循其中的关联规律,利用ArcGIS软件的叠加分析功能,查找出适宜规划的特定区域,做出分级评价,并制作出规划图.通过面积计算得到较适宜种植面积为4556.6hm2,适宜种植面积为75079.65hm2,可种植面积为12123.46hm2

  13. Cultivation-based multiplex phenotyping of human gut microbiota allows targeted recovery of previously uncultured bacteria

    DEFF Research Database (Denmark)

    Rettedal, Elizabeth; Gumpert, Heidi; Sommer, Morten

    2014-01-01

    show that carefully designed conditions enable cultivation of a representative proportion of human gut bacteria, enabling rapid multiplex phenotypic profiling. We use this approach to determine the phylogenetic distribution of antibiotic tolerance phenotypes for 16 antibiotics in the human gut...... microbiota. Based on the phenotypic mapping, we tailor antibiotic combinations to specifically select for previously uncultivated bacteria. Utilizing this method we cultivate and sequence the genomes of four isolates, one of which apparently belongs to the genus Oscillibacter; uncultivated Oscillibacter...

  14. A Moral Method? Thoughts on Cultivating Empathy through Method Acting.

    Science.gov (United States)

    Verducci, Susan

    2000-01-01

    Using Nel Noddings ethic of Care, contends that this model of empathy has elements in common with the type of empathy method actors employ. Discusses the agreements between Caring's empathy and dramatic empathy focusing on three skills method actors use and the three differences that possess moral implications. (CMK)

  15. Component analysis of cultivated ginseng, cultivated wild ginseng, and natural wild ginseng by structural parts using HPLC method

    Directory of Open Access Journals (Sweden)

    Young-Ju,Han

    2007-02-01

    Full Text Available Objectives : The aim of this experiments is to provide an objective differentiation of ginseng, Korean and Chinese cultivated wild ginseng, and natural wild ginseng through components analysis of different parts of ginseng. Methods : Comparative analyses of ginsenoside-, ginsenoside-, and ginsenosides and from the root, stem, and leaves of ginseng, Korean and Chinese cultivated wild ginseng, and natural wild ginseng were conducted using HPLC. Results : 1. For content comparison of leaves, ginseng showed highest content of ginsenoside than other samples. Natural wild ginseng showed relatively high content of ginsenosides and than other samples. 2. For content comparison of the stem, ginseng and 10 years old Chinese cultivated wild ginseng didn't contain ginsenoside . Natural wild ginseng showed higher content of ginsenosides and than other samples. 3. For content comparison of the root, ginsenoside was found only in 5 and 10 years old Korean cultivated wild ginseng. 4. Distribution of contents by the parts of ginseng was similar in ginseng and Chinese cultivated wild ginseng. Conclusions : Above experiment data can be an important indicator for the identification of ginseng, Korean and Chinese cultivated wild ginseng, and natural wild ginseng.

  16. LTCC based bioreactors for cell cultivation

    Science.gov (United States)

    Bartsch, H.; Welker, T.; Welker, K.; Witte, H.; Müller, J.

    2016-01-01

    LTCC multilayers offer a wide range of structural options and flexibility of connections not available in standard thin film technology. Therefore they are considered as material base for cell culture reactors. The integration of microfluidic handling systems and features for optical and electrical capturing of indicators for cell culture growth offers the platform for an open system concept. The present paper assesses different approaches for the creation of microfluidic channels in LTCC multilayers. Basic functions required for the fluid management in bioreactors include temperature and flow control. Both features can be realized with integrated heaters and temperature sensors in LTCC multilayers. Technological conditions for the integration of such elements into bioreactors are analysed. The temperature regulation for the system makes use of NTC thermistor sensors which serve as real value input for the control of the heater. It allows the adjustment of the fluid temperature with an accuracy of 0.2 K. The tempered fluid flows through the cell culture chamber. Inside of this chamber a thick film electrode array monitors the impedance as an indicator for the growth process of 3-dimensional cell cultures. At the system output a flow sensor is arranged to monitor the continual flow. For this purpose a calorimetric sensor is implemented, and its crucial design parameters are discussed. Thus, the work presented gives an overview on the current status of LTCC based fluid management for cell culture reactors, which provides a promising base for the automation of cell culture processes.

  17. Integrated Indicators-based Gradation of Cultivated Land Quality in Mountainous Region of Southwestern China

    Institute of Scientific and Technical Information of China (English)

    SHAO Jing'an; WEI Chaofu; XIE Deti; ZHOU Yan

    2006-01-01

    The gradation of cultivated land is to assess the suitability of cultivated land for agricultural production in terms of natural and economic properties of land. It can be used to evaluate sustainability of land use and soil management practices. Formal and informal surveys, Delphi and comprehensive index method are adopted to identify appropriate integrated indicators, and measure the gradation of cultivated land quality. The determination of integrated indicators presents three basic features of cultivated land quality: stable plant productivity, social acceptability and maintenance of environmental quality.Pronounced concentration-dissipation law of total function score value occurs in paddy field units, with three ranges, I.e., 79-68.5, 68.5~59 and 59-51. Total function score value in dryland units mainly ranges from 40 to 70, but slight concentration-dissipation law of each unit is still observed, with four ranges, I.e., 79~68, 68~51, 51~37 and 37~15. Paddy field quality is divided into three gradations, and the scales are 18 220.9 ha, 5410.6 ha and 2890.9 ha. Dryland quality is classified into four gradations, and the scales are 1548.6 ha, 8153.8 ha, 3316.3 ha and 685.2 ha. The total function score value of every gradation unit is conformed to the results of farmers' assessment. Research results meet with the needs of accurate degree of the gradation of cultivated land quality. Integrated indicators-based gradation of cultivated land assessment accords with the characteristics of land resources and human preference in the mountains of southwestern China. This study will be useful to promote the monitoring of cultivated land quality, and to supply fine ground and knowledge for establishing appropriate cultivated land management practices in Southwestern China.

  18. Cultivation of Critical Thinking Ability Based on Flipped Classroom Teaching Method%基于思辨能力培养的英美文学翻转教学改革

    Institute of Scientific and Technical Information of China (English)

    毛艳华

    2015-01-01

    At present, absence of critical thinking and humanistic literacy has become a universal phenomenon among English majors. It is necessary and urgent to develop teaching reformation of British and American literature. With the combination of technology-based and traditional teaching methods , the paper has mainly discussed how to cultivate critical thinking ability of English majors with the help of flipped classroom teaching method. The main teaching steps include inverting teaching procedure , developing teaching resources and reforming testing system.%英语专业英美文学教学中存在思辨缺席、人文缺失的普遍现象。文章从重构教学流程、强化课堂思辨,拓展教学资源、延展思辨空间,开放考核体系、释放思辨活力三方面讨论以“翻转课堂”促“思辨培养”的主要操作方法,旨在论证融合信息化教育手段与优良的文学教学传统,可以提升学生的文学思辨能力与人文素养。

  19. Comparison of the Anaerocult A and the oil blocking methods for the in vitro cultivation of Entamoeba histolytica.

    Science.gov (United States)

    Mohd Shah, Nurul Azira; Wan Abdul Wahab, Wan Nor Amilah; Mohd Nawi, Siti Farah Alwani; Mohd-Zain, Zaini; Latif, Baha; Suhaimi, Rosliza

    2015-12-01

    Entamoeba histolytica, the causative agent for human amoebiasis, is among the most deadly parasites, accounting for the second highest mortality rate among parasitic diseases. Because this parasite dwells in low oxygen tension, for its cultivation, microaerophilic conditions are required to mimick the human gut environment. Several methods developed for optimal growth environment are commercially available and some are conventionally modified in-house which include the Anaerocult A and oil blocking preparation methods. This study was undertaken to compare the reliability of the Anaerocult A and the oil blocking methods in generating anaerobic environment for cultivation of E. histolytica. The trophozoites of E. histolytica HM1: IMSS strains were axenically cultivated in TYI-S-33 medium in culture incubated anaerobically by using Anaerocult A (Merck) and mineral oil blocking method. The outcomes of both methods were determined by the minimum inhibitory concentration (MIC) of metronidazole against E. histolytica by giving a score to the growth pattern of the trophozoites. The reliability of both methods was assessed based on susceptibility testing of E. histolytica to metronidazole. The MIC obtained by both anaerobic condition methods was 6.25 ug/ ml, thus showing that oil-blocking method is comparable to the Anaerocult A method and therefore, considered as a reliable method for generating an anaerobic environment for the cultivation of E. histolytica.

  20. To Cultivate Outstanding Software Engineer Based on Project-based Learning

    Institute of Scientific and Technical Information of China (English)

    LI Dong; MA Peiqun; LIU Shu

    2012-01-01

    This thesis sketches the connotation of project-based learning and introduces the basis on which project-based learning is practiced and applied in school of software as well as the plan of further practicing project-based learning. At the same time, this thesis also discusses application of project-based learning in "education and cultivation plan of excellent engineer".

  1. Effect of plant cultivation methods on content of major and trace elements in foodstuffs and retention in rats

    DEFF Research Database (Denmark)

    Kristensen, Mette; Østergaard, Lars F.; Halekoh, Ulrich

    2008-01-01

    , there was no evident trend towards differences in element content of foodstuffs or diets due to the use of different cultivation systems, and differences between harvest years exceeded those seen between cultivation methods. Also, no significant differences in the retention of elements in rats fed diets derived from...... major and trace element contents of dried foodstuffs (carrots, kale, peas, potatoes and apples) grown in two consecutive years, as well as mineral retention determined in 36 rats (second generation in a multi-generation study) fed diets based on these foodstuffs from one year. RESULTS: Overall...... different cultivation systems were seen, since higher intake resulted in correspondingly higher excretion. CONCLUSION: This study does not support the belief that organically grown foodstuffs generally contain more major and trace elements than conventionally grown foodstuffs, nor does there appear...

  2. Production of microbial enzymes by new method of cultivation of microorganisms

    Directory of Open Access Journals (Sweden)

    Blieva Raushan

    2014-10-01

    Full Text Available We have developed efficient methods for long-term culturing and selection of highly active versions of the original cultures of micromycetes – producers of enzymes. We theoretically substantiated and experimentally confirmed an advantage of growing micromycetes in a new filament-spongy immobilized growth structure on the substrate relative to the traditional method of deep cultivation of free cells in the form of pellets. When comparing a traditional with our innovative method of cultivation, many advantages of the latter are revealed, above all being the possibility of the formation of new highly selective cultures in the long process of their growth with modified culturally - morphological properties.

  3. 浅议基于“参与”理念的“学工-教学”融合式人才培养途径——以宁波大红鹰学院为例%A Preliminary Exploration of Combination of "Student Affair Teaching" Talent Cultivation Method Based on Concept of Participation ---- Taking Ningbo Dahongying University as an Example

    Institute of Scientific and Technical Information of China (English)

    吴勇

    2012-01-01

    人才培养工作是高等院校的根本任务,以“学生为本”的教育理念已成为高校育人工作的核心指导思想,探索新时期育人工作的有效途径是高校进一步落实科学发展观的必然要求。可以传统人才培养工作中所存问题为切入点.以提高学生基础素质和能力的培养为主线,定位于培养区域中小型企业所需的高端技术和管理岗位的高素质应用型人才,以学生成才为导向.探索基于“参与”理念的“学工-教学”相融合的人才培养途径。旨在为高校在新时代背景和社会需求下,培养具有较强创新精神和实践能力的应用型人才,提供理论和实践上的参考。%Talent cultivation is the fundamental task for colleges. Student-orientation has become the guideline for college cultivation work. The exploration of new means for college's cultivation for talents is the necessity for further implementing the scientific outlook on development. We can take the problems existing in traditional cultivation work as the penetration point, take the improving basic quality and capacity of students as guideline, focus on cultivation of high quality and applicable high-end technology and managerial talents required by regional SMEs. We can also take the success of students as guideline to explore talent cultivation method combined "student affair-teaching" based on concept of participation so as to offer theoretical and practical reference for cultivation of innovative and practical applicable talents under the background of new era and social requirements for colleges.

  4. Expansion of mesenchymal stem cells using a microcarrier-based cultivation system: growth and metabolism

    NARCIS (Netherlands)

    Schop, D.; Janssen, F.W.; Borgart, E.; Bruijn, de J.D.; Dijkhuizen-Radersma, van R.

    2008-01-01

    For the continuous and fast expansion of mesenchymal stem cells (MSCs), microcarriers have gained increasing interest. The aim of this study was to evaluate the growth and metabolism profiles of MSCs, expanded in a microcarrier-based cultivation system. We investigated various cultivation conditions

  5. Curriculum-based library instruction from cultivating faculty relationships to assessment

    CERN Document Server

    Blevins, Amy

    2014-01-01

    Curriculum-Based Library Instruction: From Cultivating Faculty Relationships to Assessment highlights the movement beyond one-shot instruction sessions, specifically focusing on situations where academic librarians have developed curriculum based sessions and/or become involved in curriculum committees.

  6. Simple and efficient method for isolation and cultivation of endoscopically obtained human colonocytes

    DEFF Research Database (Denmark)

    Seidelin, Jakob B; Horn, Thomas; Nielsen, Ole H

    2003-01-01

    on a porous membrane was superior to the other methods applied [P cultivated cells. CM had similar viability supporting effects to FCS. Other supplements had......Few comparative and validated reports exist on the isolation and growth of colonoscopically obtained colonic epithelium. The aim of this study was to develop and validate a simple method for the cultivation of colonoscopically obtained colonocytes. Forty patients, who underwent routine colonoscopy...... and where the diagnosis of irritable bowel syndrome was later reached, were included. Seven colon biopsies were taken and incubated at varying time periods of 10-120 min and temperatures of 4-37 degrees C in a chelating buffer. The epithelium was then harvested and cultivated under three different...

  7. Simple and efficient method for isolation and cultivation of endoscopically obtained human colonocytes

    DEFF Research Database (Denmark)

    Seidelin, Jakob B; Horn, Thomas; Nielsen, Ole H

    2003-01-01

    on a porous membrane was superior to the other methods applied [P cultivated cells. CM had similar viability supporting effects to FCS. Other supplements had no effects......Few comparative and validated reports exist on the isolation and growth of colonoscopically obtained colonic epithelium. The aim of this study was to develop and validate a simple method for the cultivation of colonoscopically obtained colonocytes. Forty patients, who underwent routine colonoscopy...... and where the diagnosis of irritable bowel syndrome was later reached, were included. Seven colon biopsies were taken and incubated at varying time periods of 10-120 min and temperatures of 4-37 degrees C in a chelating buffer. The epithelium was then harvested and cultivated under three different...

  8. Examination of bacteriological status of surface fresh waters using direct and cultivation methods

    Directory of Open Access Journals (Sweden)

    Petrović Olga V.

    2011-01-01

    Full Text Available The number of bacteria in surface waters reflects the current state of waters and their trophicity, and it is the first parameter that is affected by the anthropogenic contamination. Traditionally, quantification of bacteria in waters is performed using the cultivation methods. However, all these methods detect only cultivable bacteria; the results depend on the incubation conditions, and the results are obtained after several days. These deficiencies are solved by using direct methods. This work is the first bacteriological examination of the reservoir Ćelije and its tributaries by using a direct method of quantification. Total of 343 samples of water from the reservoir and its tributaries were processed. In each sample, the count of aerobic mesophilic bacteria was determined using cultivation method was involving involved inoculation of samples on high-nutrient PCA medium and on low-nutrient R2A medium, and incubation for 7 days at room temperature. The total number of bacteria was determined by epifluorescence microscopy technique after filtration of samples previously stained with acridine orange. Using the cultivation methods, the highest number of bacteria was recorded in the river of Blatašnica on R2A medium (56,535 CFU/ml, and lowest in the basin Vodozahvat on PCA medium (1,364 CFU/ml. According to the results from R2A, water belonged to the class of water of poorer quality compared to the results from the PCA. The results obtained by the direct method were correlated with cultivation methods. The significantly highest number of bacteria was obtained by epifluorescence microscopy, and the lowest on PCA medium. If the application of direct method for some reason is not possible, the real results of bacteriological state of surface waters can be obtained by inoculation of samples on R2A medium with adequate incubation conditions.

  9. Quantification of encapsulated bioburden in spacecraft polymer materials by cultivation-dependent and molecular methods.

    Directory of Open Access Journals (Sweden)

    Anja Bauermeister

    Full Text Available Bioburden encapsulated in spacecraft polymers (such as adhesives and coatings poses a potential risk to jeopardize scientific exploration of other celestial bodies. This is particularly critical for spacecraft components intended for hard landing. So far, it remained unclear if polymers are indeed a source of microbial contamination. In addition, data with respect to survival of microbes during the embedding/polymerization process are sparse. In this study we developed testing strategies to quantitatively examine encapsulated bioburden in five different polymers used frequently and in large quantities on spaceflight hardware. As quantitative extraction of the bioburden from polymerized (solid materials did not prove feasible, contaminants were extracted from uncured precursors. Cultivation-based analyses revealed <0.1-2.5 colony forming units (cfu per cm3 polymer, whereas quantitative PCR-based detection of contaminants indicated considerably higher values, despite low DNA extraction efficiency. Results obtained from this approach reflect the most conservative proxy for encapsulated bioburden, as they give the maximum bioburden of the polymers irrespective of any additional physical and chemical stress occurring during polymerization. To address the latter issue, we deployed an embedding model to elucidate and monitor the physiological status of embedded Bacillus safensis spores in a cured polymer. Staining approaches using AlexaFluor succinimidyl ester 488 (AF488, propidium monoazide (PMA, CTC (5-cyano-2,3-diotolyl tetrazolium chloride demonstrated that embedded spores retained integrity, germination and cultivation ability even after polymerization of the adhesive Scotch-Weld 2216 B/A. Using the methods presented here, we were able to estimate the worst case contribution of encapsulated bioburden in different polymers to the bioburden of spacecraft. We demonstrated that spores were not affected by polymerization processes. Besides Planetary

  10. Strategies for Cultivating New Peasants Based on Modern Agricultural Development

    Institute of Scientific and Technical Information of China (English)

    Fanxing; MENG; Jianguo; ZHOU

    2013-01-01

    Development of modern agriculture has higher and higher requirements on quality of peasants.This study,on the basis of connotations of modern agriculture,current situation and problems of Chinese peasants,and contradictions between modern agricultural development and peasants’ quality,specifies requirements of modern agriculture on new peasants,proposes corresponding suggestions to promote the cultivation of new Chinese peasants.

  11. 基于农用地分等修正的土地整治项目对耕地质量的影响评价%Evaluation on influence of land consolidation project on cultivated land quality based on agricultural land classification correction method

    Institute of Scientific and Technical Information of China (English)

    匡丽花; 叶英聪; 赵小敏; 郭熙; 谢文

    2016-01-01

    natural quality would be affected by the land consolidation project. In this study, the initial grading factors could be divided into two categories, the first were the stable factors, the attribute remained the same after the land consolidation project, and the second were the changing factors, the attribute changed after the land consolidation project. For these stable factors, in this paper, we took the original data directly. For these changing factors, we would collect the data after the land consolidation project through field investigation and indoor analysis. The weights and scoring rules of the natural quality grading factors stayed the same. Then we recalculated the natural quality index again. The canonical correlation analysis method was used to determine the land use correction factors from the influence factors of land consolidation project on the cultivated land production conditions. Then, we determined the weights of the factors on the base of Delphi method and analytic hierarchy process (AHP). The ratio of the scores before and after land consolidation was the land use correction coefficient and modified the original land use coefficient so that the revised land use index could be derived according to the original evaluation process. In the paper, we developed the cultivated land quality natural grade and the land use grade according to Agricultural Land Classification for Jiangxi Province. Then we compared and analyzed the changing situation of cultivated land quality grade before and after land consolidation. At last, in this paper, we used the multiple regression analysis methods to determine correction factors which had significant influence on the cultivated land quality. The land use correction factors were plots regularity, road network density, effective irrigation area index, distribution of the drought and flood. The results showed that the average natural quality grade were raised from 9.06 to 8.81 after the land consolidation, the general

  12. Teaching Methods for Science and Technology: Visualized Teaching and Cultivating the Capacity for Scientific Abstraction

    Science.gov (United States)

    Chinese Education, 1973

    1973-01-01

    Improvement of the student's ability to analyze and solve problems by effectively relating the use of visual aids to the cultivation of capacity for scientific abstraction is discussed. These basic theory courses are based on the dialectical materialistic theory of knowledge and Mao's instruction on the unity of theory and reality.'' (SM)

  13. Design and Implementation of Emergy-Based Sustainability Decision Assessment System for Protected Grape Cultivation

    Directory of Open Access Journals (Sweden)

    Jianying Feng

    2015-10-01

    Full Text Available The characteristic “high input and output” in protected production has caused some environmental and ecological issues. Hence, emergy-based sustainability assessments are necessary and valuable. However, traditional emergy analysis is time consuming, tedious, and inefficient. Such disadvantages can be addressed by the integration of emergy analysis with information technology. This paper reports the development of the emergy-based sustainability decision assessment system (ESDAS for protected grape cultivation systems. This system was established by first analyzing the business process, users, and requirements through survey, and the findings of which were used to design the system’s function, architecture, database, model base, and knowledge base with a combination of emergy methods. The results showed that ESDAS passed the system test and achieved the real-time calculation of emergy data and the automatization of emergy analysis. Therefore, this research is a beneficial attempt to apply information technology in improving the efficiency of sustainability assessments. The results also revealed that the protected grape cultivation system is characterized by a heavy dependence on purchased and non-renewable resource emergy, lower emergy yield ratio, higher emergy investment rate and environmental loading ratio, and lower emergy sustainability index. Some suggestions were made to improve the sustainability of the protected grape system.

  14. Simple and efficient method for isolation and cultivation of endoscopically obtained human colonocytes.

    Science.gov (United States)

    Seidelin, Jakob B; Horn, Thomas; Nielsen, Ole H

    2003-12-01

    Few comparative and validated reports exist on the isolation and growth of colonoscopically obtained colonic epithelium. The aim of this study was to develop and validate a simple method for the cultivation of colonoscopically obtained colonocytes. Forty patients, who underwent routine colonoscopy and where the diagnosis of irritable bowel syndrome was later reached, were included. Seven colon biopsies were taken and incubated at varying time periods of 10-120 min and temperatures of 4-37 degrees C in a chelating buffer. The epithelium was then harvested and cultivated under three different conditions: 1) on a collagen coating, 2) embedded in a collagen gel, or 3) embedded in a gel put on a porous well insert. The effect of conditioned medium (CM), insulin, transferrin, selenium, and the oxygen content was assessed. Viability was tested by the metabolic dimethylthiazol-diphenyl-tetrazolium bromide assay, by flowcytometry, by phase contrast microscopy, and by transmission electron microscopy. Incubation at 21 degrees C for 75 min gave an optimal yield of 3 x 10(6) (2.0-3.8 x 10(6)) viable epithelial cells in intact crypts per seven biopsies. Embedding of crypts in a collagen gel put on a porous membrane was superior to the other methods applied [P coat-cultivated cells. CM had similar viability supporting effects to FCS. Other supplements had no effects. A simple method is presented, which makes cultivation of colonocytes obtained at endoscopy possible for up to 72 h.

  15. Construction of the Classification and Grading Index System of Cultivated Land Based on the Viewpoint of Sustainable Development

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    In order to objectively and reasonably evaluate the actual and potential value of cultivated land, both social and ecological values are introduced into the classification and grading index system of cultivated land based on the viewpoint of sustainable development, after considering the natural and economic values of cultivated land. Index system construction of the sustainable utilization of cultivated land should follow the principles of economic viability, social acceptability, and ecological protection. Classification of cultivated land should take into account the soil fertility of cultivated land. Then, grading of cultivated land is carried out from the practical productivity (or potential productivity) of cultivated land. According to the existing classification index system of cultivated land, the soil, natural and environmental factors in plains, mountains and hills are mainly modified in the classification index system of cultivated land. And index systems for the cultivated land classification in plains, mountains and hills are set up. The grading index system of cultivated land is established based on the economic viability (economic value), social acceptability (social value) and protection of cultivated land (ecological value). Quantitative expression of cultivated land grading index is also carried out.

  16. Validation of a novel sequential cultivation method for the production of enzymatic cocktails from Trichoderma strains.

    Science.gov (United States)

    Florencio, C; Cunha, F M; Badino, A C; Farinas, C S

    2015-02-01

    The development of new cost-effective bioprocesses for the production of cellulolytic enzymes is needed in order to ensure that the conversion of biomass becomes economically viable. The aim of this study was to determine whether a novel sequential solid-state and submerged fermentation method (SF) could be validated for different strains of the Trichoderma genus. Cultivation of the Trichoderma reesei Rut-C30 reference strain under SF using sugarcane bagasse as substrate was shown to be favorable for endoglucanase (EGase) production, resulting in up to 4.2-fold improvement compared with conventional submerged fermentation. Characterization of the enzymes in terms of the optimum pH and temperature for EGase activity and comparison of the hydrolysis profiles obtained using a synthetic substrate did not reveal any qualitative differences among the different cultivation conditions investigated. However, the thermostability of the EGase was influenced by the type of carbon source and cultivation system. All three strains of Trichoderma tested (T. reesei Rut-C30, Trichoderma harzianum, and Trichoderma sp INPA 666) achieved higher enzymatic productivity when cultivated under SF, hence validating the proposed SF method for use with different Trichoderma strains. The results suggest that this bioprocess configuration is a very promising development for the cellulosic biofuels industry.

  17. Cultivating Life Skills at a Project-Based Charter School

    Science.gov (United States)

    Wurdinger, Scott; Enloe, Walter

    2011-01-01

    Surveys that focused on academic and life skill development were collected from alumni who attended Avalon Charter School in St Paul, Minnesota. Avalon is a small public charter school that uses project-based learning as their primary teaching method. Forty-two alumni responded to the online survey. Students ranked life skills such as creativity,…

  18. Modeling microalgae cultivation productivities in different geographic locations - estimation method for idealized photobioreactors.

    Science.gov (United States)

    Franz, Anette; Lehr, Florian; Posten, Clemens; Schaub, Georg

    2012-04-01

    Microalgae can be used to produce versatile high-value fuels, such as methane, biodiesel, ethanol, or hydrogen gas. One of the most important factors that influence the economics of microalgae cultivation is the primary production of biomass per unit area. This is determined by productivity rates during cultivation, which are influenced by the local climate conditions (solar irradiation, temperature). To compare locations in different climate regions for microalgae cultivation, a mathematical model for an idealized closed photobioreactor was developed. The applied growth kinetics were based on theoretical maximum photon-conversion efficiencies (for the conversion of solar energy to chemical energy in the form of biomass). Known or estimated temperature effects for different algal strains were incorporated. The model was used to calculate hourly average areal productivity rates as well as annual primary production values under local conditions at seven example locations. Here, hourly weather data (solar irradiance and air temperature) were taken into account. According to these model calculations, maximum annual yields were achieved in regions with high irradiation and temperature patterns in or near the optimum range of the specific algal strain (here, desert and equatorial humid climates). The developed model can be used as a tool to assess and compare individual locations for microalgae cultivation.

  19. Exploration of the Strategies of Cultivating Students' Critical Thinking Ability in Col ege English Education in the Network Environment--A Perspective of Task-based Teaching Method%网络环境下大学英语教学中批判性思维培养策略探索--基于任务型教学法的视角

    Institute of Scientific and Technical Information of China (English)

    王大智

    2014-01-01

    This article explores how to cultivate student's critical thinking ability in college English teaching process in the network envi-ronment with the help of task-based teaching method through examples. Meanwhile, it also analyzes the advantages of task-based teach-ing method in developing students' critical thinking ability and states some issues that need attention.%文章通过例证探讨了在网络环境下,基于任务型教学法的大学英语教学中如何进行批判性思维培养,分析了任务型教学在培养批判性思维方面的优势,并提出了在培养学生批判性思维时应注意的问题。

  20. Estimation of the sugar cane cultivated area from LANDSAT images using the two phase sampling method

    Science.gov (United States)

    Parada, N. D. J. (Principal Investigator); Cappelletti, C. A.; Mendonca, F. J.; Lee, D. C. L.; Shimabukuro, Y. E.

    1982-01-01

    A two phase sampling method and the optimal sampling segment dimensions for the estimation of sugar cane cultivated area were developed. This technique employs visual interpretations of LANDSAT images and panchromatic aerial photographs considered as the ground truth. The estimates, as a mean value of 100 simulated samples, represent 99.3% of the true value with a CV of approximately 1%; the relative efficiency of the two phase design was 157% when compared with a one phase aerial photographs sample.

  1. A method to estimate the biomass of Spirulina platensis cultivated on a solid medium

    OpenAIRE

    Lúcia Helena Pelizer; Iracema de Oliveira Moraes

    2014-01-01

    This paper presents a method to estimate the biomass of Spirulina cultivated on solid medium with sugarcane bagasse as a support, in view of the difficulty in determining biomass concentrations in bioprocesses, particularly those conducted in semi-solid or solid media. The genus Spirulina of the family Oscillatoriaceae comprises the group of multicellular filamentous cyanobacteria (blue-green microalgae). Spirulina is used as fish feed in aquaculture, as a food supplement, a source of vitamin...

  2. An alternative method to estimate WiP inventory for cultivated assets

    OpenAIRE

    野村, 浩二

    2006-01-01

    The objectives of this paper are to describe the problems with the current estimates for WiP inventories of cultivated assets in Japan's system of national accounts and to propose an alternative method of dealing with these assets. There is a huge di erence between inventory stock measurement that are compiled according to the 1968 SNA versus the 1993 SNA for Japan's national accounts. This leads to non-negligible di erences for productivity measures when inventories are treated as a capital ...

  3. A method to estimate the biomass of Spirulina platensis cultivated on a solid medium

    Directory of Open Access Journals (Sweden)

    Lúcia Helena Pelizer

    2014-09-01

    Full Text Available This paper presents a method to estimate the biomass of Spirulina cultivated on solid medium with sugarcane bagasse as a support, in view of the difficulty in determining biomass concentrations in bioprocesses, particularly those conducted in semi-solid or solid media. The genus Spirulina of the family Oscillatoriaceae comprises the group of multicellular filamentous cyanobacteria (blue-green microalgae. Spirulina is used as fish feed in aquaculture, as a food supplement, a source of vitamins, pigments, antioxidants and fatty acids. Therefore, its growth parameters are extremely important in studies of the development and optimization of bioprocesses. For studies of biomass growth, Spirulina platensis was cultured on solid medium using sugarcane bagasse as a support. The biomass thus produced was estimated by determining the protein content of the material grown during the process, based on the ratio of dry weight to protein content obtained in the surface growth experiments. The protein content of the biomass grown in Erlenmeyer flasks on surface medium was examined daily to check the influence of culture time on the protein content of the biomass. The biomass showed an average protein content of 42.2%. This methodology enabled the concentration of biomass adhering to the sugarcane bagasse to be estimated from the indirect measurement of the protein content associated with cell growth.

  4. A method to estimate the biomass of Spirulina platensis cultivated on a solid medium

    Science.gov (United States)

    Pelizer, Lúcia Helena; Moraes, Iracema de Oliveira

    2014-01-01

    This paper presents a method to estimate the biomass of Spirulina cultivated on solid medium with sugarcane bagasse as a support, in view of the difficulty in determining biomass concentrations in bioprocesses, particularly those conducted in semi-solid or solid media. The genus Spirulina of the family Oscillatoriaceae comprises the group of multicellular filamentous cyanobacteria (blue-green microalgae). Spirulina is used as fish feed in aquaculture, as a food supplement, a source of vitamins, pigments, antioxidants and fatty acids. Therefore, its growth parameters are extremely important in studies of the development and optimization of bioprocesses. For studies of biomass growth, Spirulina platensis was cultured on solid medium using sugarcane bagasse as a support. The biomass thus produced was estimated by determining the protein content of the material grown during the process, based on the ratio of dry weight to protein content obtained in the surface growth experiments. The protein content of the biomass grown in Erlenmeyer flasks on surface medium was examined daily to check the influence of culture time on the protein content of the biomass. The biomass showed an average protein content of 42.2%. This methodology enabled the concentration of biomass adhering to the sugarcane bagasse to be estimated from the indirect measurement of the protein content associated with cell growth. PMID:25477928

  5. A method to estimate the biomass of Spirulina platensis cultivated on a solid medium.

    Science.gov (United States)

    Pelizer, Lúcia Helena; Moraes, Iracema de Oliveira

    2014-01-01

    This paper presents a method to estimate the biomass of Spirulina cultivated on solid medium with sugarcane bagasse as a support, in view of the difficulty in determining biomass concentrations in bioprocesses, particularly those conducted in semi-solid or solid media. The genus Spirulina of the family Oscillatoriaceae comprises the group of multicellular filamentous cyanobacteria (blue-green microalgae). Spirulina is used as fish feed in aquaculture, as a food supplement, a source of vitamins, pigments, antioxidants and fatty acids. Therefore, its growth parameters are extremely important in studies of the development and optimization of bioprocesses. For studies of biomass growth, Spirulina platensis was cultured on solid medium using sugarcane bagasse as a support. The biomass thus produced was estimated by determining the protein content of the material grown during the process, based on the ratio of dry weight to protein content obtained in the surface growth experiments. The protein content of the biomass grown in Erlenmeyer flasks on surface medium was examined daily to check the influence of culture time on the protein content of the biomass. The biomass showed an average protein content of 42.2%. This methodology enabled the concentration of biomass adhering to the sugarcane bagasse to be estimated from the indirect measurement of the protein content associated with cell growth.

  6. Comparison of algae cultivation methods for bioenergy production using a combined life cycle assessment and life cycle costing approach.

    Science.gov (United States)

    Resurreccion, Eleazer P; Colosi, Lisa M; White, Mark A; Clarens, Andres F

    2012-12-01

    Algae are an attractive energy source, but important questions still exist about the sustainability of this technology on a large scale. Two particularly important questions concern the method of cultivation and the type of algae to be used. This present study combines elements of life cycle analysis (LCA) and life cycle costing (LCC) to evaluate open pond (OP) systems and horizontal tubular photobioreactors (PBRs) for the cultivation of freshwater (FW) or brackish-to-saline water (BSW) algae. Based on the LCA, OPs have lower energy consumption and greenhouse gas emissions than PBRs; e.g., 32% less energy use for construction and operation. According to the LCC, all four systems are currently financially unattractive investments, though OPs are less so than PBRs. BSW species deliver better energy and GHG performance and higher profitability than FW species in both OPs and PBRs. Sensitivity analyses suggest that improvements in critical cultivation parameters (e.g., CO(2) utilization efficiency or algae lipid content), conversion parameters (e.g., anaerobic digestion efficiency), and market factors (e.g., costs of CO(2) and electricity, or sale prices for algae biodiesel) could alter these results.

  7. Genetic Diversity Based on Allozyme Alleles of Chinese Cultivated Rice

    Institute of Scientific and Technical Information of China (English)

    TANG Sheng-xiang; WEI Xing-hua; JIANG Yun-zhu; D S Brar; G S Khush

    2007-01-01

    Genetic diversity was analyzed with 6 632 core rice cultivars selected from 60 282 Chinese rice accessions on the basis of 12 allozyme loci, Pgil, Pgi2, Ampl, Amp2, Amp3, Amp4, Sdh1, Adh1, Est1, Est2, Est5 and Est9, by starch gel electrophoresis. Among the materials examined, 52 alleles at 12 polymorphic loci were identified, which occupied 96.3% of 54 alleles found in cultivated germplasm of O.sativa L. The number of alleles per locus ranged from 2 to 7 with an average of 4.33. The gene diversity (He) each locus varied considerably from 0.017 for Amp4 to 0.583 for Est2 with an average gene diversity (Ht) 0.271, and Shannon-Wiener index from 0.055 to 0.946 with an average of 0.468. The degree of polymorphism (DP) was in a range from 0.9 to 46.9% with an average of 21.4%. It was found that the genetic diversity in japonica (Keng) subspecies was lower in terms of allele's number, Ht and S-W index, being 91.8, 66.2 and 75.7% of indica (Hsien) one, respectively. Significant genetic differentiation between indica and japonica rice has been appeared in the loci Pgil, Amp2, Pgi2, and Est2, with higher average coefficient of genetic differentiation (Gst) 0.635, 0.626, 0.322 and 0.282, respectively. Except less allele number per locus (3.33) for modern cultivars, being 76.9% of landraces, the Ht and S-W index showed in similar between the modern cultivars and the landraces detected. In terms of allozyme, the rice cultivars in the Southwest Plateau and Central China have richer genetic diversity. The present study reveals again that Chinese cultivated rice germplasm has rich genetic diversity, showed by the allozyme allele variation.

  8. Water Productivity of Irrigated Rice under Transplanting, Wet Seeding and Dry Seeding Methods of Cultivation

    Directory of Open Access Journals (Sweden)

    Murali, NS.

    1997-01-01

    Full Text Available Water productivity (WP of irrigated lowland rice was determined during the 1994 dry (January to May and wet (August to December seasons on a heavy clay acid sulphate soil. Treatments consisted of three cultivation methods : transplanted rice, pregerminated seeds broadcasted on puddled soil (wet seeding and dry seeds broadcasted on unpuddled soil (dry seeding. In wet and dry seeded plots, continuous standing water condition was initiated 17 days after sowing. Total water requirement for rice production was highest in transplanted plots (755 mm in wet season and 1154 mm in dry season and was lowest in dry seeded plots (505 mm in wet season and 1040 mm in dry season. Dry seeding required no water for land preparation but transplanting and wet seeding methods required 18 - 20 % of total water requirement in dry season and 27 - 29 % in wet season. Total percolation was maximum (99 mm in wet season and 215 mm in dry season in dry seeding method and was minimum (62 mm in wet season and 94 mm in dry season in transplanting method. In dry and wet seeding methods, daily percolation gradually decreased with the age of the crop. Total seepage loss did not show any significant difference between the cultivation methods in the two seasons. Grain yield was not affected by the three cultivation methods in both seasons. Water productivity (the ratio between grain yield and total amount of water used in production was 3.5 - 4.1 kg ha-1 mm-1, 3.8 - 4.4 kg ha-1 mm-1 and 4.1 - 5.5 kg ha-1 mm-1 in transplanted, wet seeded and dry seeded rice, respectively. Labour requirement for land preparation and sowing was maximum in transplanted (219 - 226 man-hours ha-1 followed by wet (104 -112 man-hours ha-1 and dry seeded (94 - 99 man-hours ha-1 methods. However, in wet season extra labour (77 man-hours ha-1 was required for weeding after crop establishment in dry and wet seeding methods. Crop maturity was 20 days earlier in wet and dry seeding methods compared to

  9. 一种分离培养硫酸盐还原菌的改进方法%A IMPROVED METHOD FOR ISLATION AND CULTIVATION OF SULFATE-REDUCING BACTERIA

    Institute of Scientific and Technical Information of China (English)

    万海清; 苏仕军; 葛长海; 万雪松; 朱家骅

    2003-01-01

    On the base of the characteristics of sulfate-reducing bacteria (SRB) growth and dish cultivated method, a new method for isolation and cultivation of SRB is established. It is dilution spread and repeat dish sandwish cultivated method with the exellence of dilution spread and embeded growth, and it can help separation and identification of SRB. The method has simple operation and less equipment. And using this method, the natural SRB drop was successfully gained, and small agar pieces containing SRB drop were acquired in easiness. The brief SRB transfering operation in strict anaerobic condition was in-deed realized. Fig 1, Ref 7.

  10. Seasonal cultivated and fallow cropland mapping using MODIS-based automated cropland classification algorithm

    Science.gov (United States)

    Wu, Zhuoting; Thenkabail, Prasad S.; Mueller, Rick; Zakzeski, Audra; Melton, Forrest; Johnson, Lee; Rosevelt, Carolyn; Dwyer, John; Jones, Jeanine; Verdin, James P.

    2013-01-01

    Increasing drought occurrences and growing populations demand accurate, routine, and consistent cultivated and fallow cropland products to enable water and food security analysis. The overarching goal of this research was to develop and test automated cropland classification algorithm (ACCA) that provide accurate, consistent, and repeatable information on seasonal cultivated as well as seasonal fallow cropland extents and areas based on the Moderate Resolution Imaging Spectroradiometer remote sensing data. Seasonal ACCA development process involves writing series of iterative decision tree codes to separate cultivated and fallow croplands from noncroplands, aiming to accurately mirror reliable reference data sources. A pixel-by-pixel accuracy assessment when compared with the U.S. Department of Agriculture (USDA) cropland data showed, on average, a producer’s accuracy of 93% and a user’s accuracy of 85% across all months. Further, ACCA-derived cropland maps agreed well with the USDA Farm Service Agency crop acreage-reported data for both cultivated and fallow croplands with R-square values over 0.7 and field surveys with an accuracy of ≥95% for cultivated croplands and ≥76% for fallow croplands. Our results demonstrated the ability of ACCA to generate cropland products, such as cultivated and fallow cropland extents and areas, accurately, automatically, and repeatedly throughout the growing season.

  11. An Inquiry into the Cultivation of Students' Creative Learning Method%培养学生创造性的学习方法

    Institute of Scientific and Technical Information of China (English)

    谭美玲

    2011-01-01

    高等学校是培养学生创造性精神和实用型人才的基地,是科学文化发展和促进和谐社会建设的重要场所。高校要通过不断的教学改革,培养学生的素质教养,努力培养学生的创造性思维。本文主要是介绍创造的内涵、培养创造性学习方法的意义及如何培养创造性学习方法等方面内容。%The college is a base of cultivating students' creative spirit and cultivating practical talents,and it is also an important position of developing scientific culture and promoting the construction of harmonious society.The college should cultivate students' quality and their creative thinking through constant teaching reforms.This paper mainly introduces the definition of creativity,the meaning of cultivating creative learning method and how to cultivate creative learning method.

  12. Cultivation-Independent Methods Reveal Differences among Bacterial Gut Microbiota in Triatomine Vectors of Chagas Disease

    Science.gov (United States)

    da Mota, Fabio Faria; Marinho, Lourena Pinheiro; Moreira, Carlos José de Carvalho; Lima, Marli Maria; Mello, Cícero Brasileiro; Garcia, Eloi Souza; Carels, Nicolas; Azambuja, Patricia

    2012-01-01

    Background Chagas disease is a trypanosomiasis whose agent is the protozoan parasite Trypanosoma cruzi, which is transmitted to humans by hematophagous bugs known as triatomines. Even though insecticide treatments allow effective control of these bugs in most Latin American countries where Chagas disease is endemic, the disease still affects a large proportion of the population of South America. The features of the disease in humans have been extensively studied, and the genome of the parasite has been sequenced, but no effective drug is yet available to treat Chagas disease. The digestive tract of the insect vectors in which T. cruzi develops has been much less well investigated than blood from its human hosts and constitutes a dynamic environment with very different conditions. Thus, we investigated the composition of the predominant bacterial species of the microbiota in insect vectors from Rhodnius, Triatoma, Panstrongylus and Dipetalogaster genera. Methodology/Principal Findings Microbiota of triatomine guts were investigated using cultivation-independent methods, i.e., phylogenetic analysis of 16s rDNA using denaturing gradient gel electrophoresis (DGGE) and cloned-based sequencing. The Chao index showed that the diversity of bacterial species in triatomine guts is low, comprising fewer than 20 predominant species, and that these species vary between insect species. The analyses showed that Serratia predominates in Rhodnius, Arsenophonus predominates in Triatoma and Panstrongylus, while Candidatus Rohrkolberia predominates in Dipetalogaster. Conclusions/Significance The microbiota of triatomine guts represents one of the factors that may interfere with T. cruzi transmission and virulence in humans. The knowledge of its composition according to insect species is important for designing measures of biological control for T. cruzi. We found that the predominant species of the bacterial microbiota in triatomines form a group of low complexity whose structure

  13. Cultivation-independent methods reveal differences among bacterial gut microbiota in triatomine vectors of Chagas disease.

    Directory of Open Access Journals (Sweden)

    Fabio Faria da Mota

    Full Text Available BACKGROUND: Chagas disease is a trypanosomiasis whose agent is the protozoan parasite Trypanosoma cruzi, which is transmitted to humans by hematophagous bugs known as triatomines. Even though insecticide treatments allow effective control of these bugs in most Latin American countries where Chagas disease is endemic, the disease still affects a large proportion of the population of South America. The features of the disease in humans have been extensively studied, and the genome of the parasite has been sequenced, but no effective drug is yet available to treat Chagas disease. The digestive tract of the insect vectors in which T. cruzi develops has been much less well investigated than blood from its human hosts and constitutes a dynamic environment with very different conditions. Thus, we investigated the composition of the predominant bacterial species of the microbiota in insect vectors from Rhodnius, Triatoma, Panstrongylus and Dipetalogaster genera. METHODOLOGY/PRINCIPAL FINDINGS: Microbiota of triatomine guts were investigated using cultivation-independent methods, i.e., phylogenetic analysis of 16s rDNA using denaturing gradient gel electrophoresis (DGGE and cloned-based sequencing. The Chao index showed that the diversity of bacterial species in triatomine guts is low, comprising fewer than 20 predominant species, and that these species vary between insect species. The analyses showed that Serratia predominates in Rhodnius, Arsenophonus predominates in Triatoma and Panstrongylus, while Candidatus Rohrkolberia predominates in Dipetalogaster. CONCLUSIONS/SIGNIFICANCE: The microbiota of triatomine guts represents one of the factors that may interfere with T. cruzi transmission and virulence in humans. The knowledge of its composition according to insect species is important for designing measures of biological control for T. cruzi. We found that the predominant species of the bacterial microbiota in triatomines form a group of low

  14. Research on County-level Cultivated Land Classification Update Methods Based on the Fuzzy Matter-element and Kriging Interpolation Method%基于模糊灰色物元与克里格插值的县级耕地质量分等更新方法研究

    Institute of Scientific and Technical Information of China (English)

    曹隽隽; 周勇; 叶青清; 郧文聚; 冷国勇

    2012-01-01

    According to the characteristics of changes of cultivated land and the cycle of index change, author picks up 14 indexes considering the chemical factor, physical factor and management factor, using these indexes to construct the farmland quality dynamic monitoring indicator system. Then, the author use fuzzy grey matter-element method and Kriging interpolation to make an empirical study on the cultivated land grade update in Gongan county. Research conclusions: ①The overall quality of cultivated land in Gongan County is in slight decline which mainly concentrated in the eastern part of the county and near the lakes. The quality of cultivated land in land treatment and local high-yielding farmland has partially promoted. ②Fuzzy grey matter-element analysis combined with Kriging interpolation could make a better balance of contradictions of cultivated land quality dynamic update between the timeliness, accuracy and affordability.%依据耕地变化特征与指标变化周期,从化学因子、物理因子和管理因子三个方面选取14个指标构建耕地质量动态监测指标体系,运用模糊灰色物元与克里格插值方法对公安县耕地等别更新进行了实证研究。结果表明:①公安县耕地整体质量略有下降,主要集中在东部县城及湖泊附近,土地整治与高产农田区耕地质量局部提升;②模糊灰色物元分析结合克里格插值能较好的平衡耕地质量动态更新的时效性、准确性与经济性之间的矛盾。

  15. Crossing Methods and Cultivation Conditions for Rapid Production of Segregating Populations in Three Grain Amaranth Species.

    Science.gov (United States)

    Stetter, Markus G; Zeitler, Leo; Steinhaus, Adrian; Kroener, Karoline; Biljecki, Michelle; Schmid, Karl J

    2016-01-01

    Grain amaranths (Amaranthus spp.) have been cultivated for thousands of years in Central and South America. Their grains are of high nutritional value, but the low yield needs to be increased by selection of superior genotypes from genetically diverse breeding populations. Amaranths are adapted to harsh conditions and can be cultivated on marginal lands although little is known about their physiology. The development of controlled growing conditions and efficient crossing methods is important for research on and improvement of this ancient crop. Grain amaranth was domesticated in the Americas and is highly self-fertilizing with a large inflorescence consisting of thousands of very small flowers. We evaluated three different crossing methods (open pollination, hot water emasculation and hand emasculation) for their efficiency in amaranth and validated them with genetic markers. We identified cultivation conditions that allow an easy control of flowering time by day length manipulation and achieved flowering times of 4 weeks and generation times of 2 months. All three different crossing methods successfully produced hybrid F1 offspring, but with different success rates. Open pollination had the lowest (10%) and hand emasculation the highest success rate (74%). Hot water emasculation showed an intermediate success rate (26%) with a maximum of 94% success. It is simple to perform and suitable for a more large-scale production of hybrids. We further evaluated 11 single nucleotide polymorphism (SNP) markers and found that they were sufficient to validate all crosses of the genotypes used in this study for intra- and interspecific hybridizations. Despite its very small flowers, crosses in amaranth can be carried out efficiently and evaluated with inexpensive SNP markers. Suitable growth conditions strongly reduce the generation time and allow the control of plant height, flowering time, and seed production. In combination, this enables the rapid production of segregating

  16. Crossing methods and cultivation conditions for rapid production of segregating populations in three grain amaranth species

    Directory of Open Access Journals (Sweden)

    Markus G Stetter

    2016-06-01

    Full Text Available Grain amaranths (Amaranthus spp. have been cultivated for thousands of years in Central and South America. Their grains are of high nutritional value, but the low yield needs to be increased by selection of superior genotypes from genetically diverse breeding populations. Amaranths are adapted to harsh conditions and can be cultivated on marginal lands although little is known about their physiology. The development of controlled growing conditions and efficient crossing methods is important for research on and improvement of this ancient crop. Grain amaranth was domesticated in the Americas and is highly self-fertilizing with a large inflorescence consisting of thousands of very small flowers. We evaluated three different crossing methods (open pollination, hot water emasculation and hand emasculation for their efficiency in amaranth and validated them with genetic markers. We identified cultivation conditions that allow an easy control of flowering time by manipulating day length and achieved flowering times of four weeks and generation times of two months. All three different crossing methods successfully produced hybrid F1 offspring, but with different success rates. Open pollination had the lowest (10% and hand emasculation the highest success rate (74%. Hot water emasculation showed an intermediate success rate (26% with a maximum of 94% success. It is simple to perform and suitable for a more large-scale production of hybrids. We further evaluated 11 single nucleotide polymorphism (SNP markers and found that they were sufficient to validate all crosses of the genotypes used in this study for intra- and interspecific hybridisations. Despite its very small flowers, crosses in amaranth can be carried out efficiently and evaluated with inexpensive SNP markers. Suitable growth conditions strongly reduce the generation time and allow the control of plant height, flowering time and seed production. In combination, this enables the rapid

  17. Work-Based Research Degrees: Systematic Cultivation through a University-Industry Network Space

    Science.gov (United States)

    Sense, Andrew J.

    2016-01-01

    The purpose of this paper is to expound on an innovative approach to cultivating work-based Doctorates and Masters of Philosophy degrees, which involves close collaboration between industry organisations and a tertiary institution. This paper also reports on an examination of the programme's structural ability to help develop the learning…

  18. On the Cultivation of Automation Majors' Research Innovation Ability Based on Scientific Research Projects

    Science.gov (United States)

    Wang, Lipeng; Li, Mingqiu

    2012-01-01

    Currently, it has become a fundamental goal for the engineering major to cultivate high-quality engineering technicians with innovation ability in scientific research which is an important academic ability necessary for them. This paper mainly explores the development of comprehensive and designing experiments in automation based on scientific…

  19. On Cultivation Methods of College Student Laboratory Assistants Based on Self-managing Mode%基于自主管理模式的高校机房学生管理员培养方法研究

    Institute of Scientific and Technical Information of China (English)

    卢荣辉; 梁洪涛

    2011-01-01

    计算机机房是高校重要的实践教学场所,用户对机房的服务需求不断提高。通过自适应学习能力、团队协作能力的培养,建设了一支具有自我管理、自主学习能力的学生管理员队伍。学生管理员协助老师提高机房管理和维护效率的同时,其自身的计算机专业实践能力和综合素质得到了提高。%With computer laboratory becoming an important place of practical teaching in college,user's requirements of service provided have also increased greatly.Through the cultivation of their adaptive learning ability and team working ability,an efficient team of student lab assistants can be built who are good at self-management and self-learning.In this way,students can not only help teachers improve the efficiency of laboratory management and maintenance,but also improve their own practical abilities in computer and integrated qualities.

  20. Paper-based device for separation and cultivation of single microalga.

    Science.gov (United States)

    Chen, Chih-Chung; Liu, Yi-Ju; Yao, Da-Jeng

    2015-12-01

    Single-cell separation is among the most useful techniques in biochemical research, diagnosis and various industrial applications. Microalgae species have great economic importance as industrial raw materials. Microalgae species collected from environment are typically a mixed and heterogeneous population of species that must be isolated and purified for examination and further application. Conventional methods, such as serial dilution and a streaking-plate method, are intensive of labor and inefficient. We developed a paper-based device for separation and cultivation of single microalga. The fabrication was simply conducted with a common laser printer and required only a few minutes without lithographic instruments and clean-room. The driving force of the paper device was simple capillarity without a complicated pump connection that is part of most devices for microfluidics. The open-structure design of the paper device makes it operable with a common laboratory micropipette for sample transfer and manipulation with a naked eye or adaptable to a robotic system with functionality of high-throughput retrieval and analysis. The efficiency of isolating a single cell from mixed microalgae species is seven times as great as with a conventional method involving serial dilution. The paper device can serve also as an incubator for microalgae growth on simply rinsing the paper with a growth medium. Many applications such as highly expressed cell selection and various single-cell analysis would be applicable.

  1. Support system based on GIS and weighted sum method for drawing up of land suitability map for agriculture. Application to durum wheat cultivation in the area of Mleta (Algeria)

    Energy Technology Data Exchange (ETDEWEB)

    Mendas, A.; Delali, A.

    2012-11-01

    Due to constant decrease in farmlands, it is important to identify the best lands for sustainable agriculture (productive and profitable agriculture that protects the environment and that is socially equitable). This requirement has resulted in the development of land suitability maps for agriculture by combining a range of factors. Spatial analysis approaches, based on the concepts of the weighted sum, combined with Geographical Information Systems (GIS) offer the opportunity to efficiently produce these land suitability maps. The functions of the weighted sum make it possible to assign numerical weights, to distinguish between positive and negative criteria and to rank alternatives. A spatial decision support system has been developed for establishing the land suitability map for agriculture. It incorporates a version of the weighted sum method SAW (Simple Additive Weighting), applicable to the vector data model, in ArcGIS within the GIS program package environment. This approach has been tested on the area of Mleta (Algeria) to assess the land suitability for durum wheat agriculture. The parameters and the classification system used in this work are inspired from the FAO. The coherence of results confirms the system effectiveness. (Author) 23 refs.

  2. Methods for the cultivation of ciliated protozoa from the large intestine of horses.

    Science.gov (United States)

    Bełżecki, Grzegorz; Miltko, Renata; Michałowski, Tadeusz; McEwan, Neil R

    2016-01-01

    This paper describes cultivation methods for ciliates from the digestive tract of horses. Members of three different genera were successfully grown in vitro for short periods of time. However, only cells belonging to the genus Blepharocorys, which resides in the horse's large intestine, were maintained for longer periods. This Blepharocorys culture was successfully grown in vitro after inoculation of freshly excreted horse faeces in culture medium containing a population of bacteria. The ciliates survived for over six months, and the density of their population varied between 1.7 × 10(3) and 2.4 × 10(3) cells mL(-1). Favourable conditions for the prolonged cultivation of this ciliate were observed when the medium was prepared by mixing horse faeces and 'caudatum' salt solution in a 1:1 V/V ratio together with food (60% powdered meadow hay, 16% wheat gluten, 12% barley flour and 12% microcrystalline cellulose) supplied as 0.20 mg mL(-1) culture per day.

  3. Preferences based Control Design of Complex Fed-batch Cultivation Process

    Directory of Open Access Journals (Sweden)

    Yuri Pavlov

    2009-08-01

    Full Text Available In the paper is presented preferences based control design and stabilization of the growth rate of fed-batch cultivation processes. The control is based on an enlarged Wang-Monod-Yerusalimsky kinetic model. Expected utility theory is one of the approaches for utilization of conceptual information (expert preferences. In the article is discussed utilization of stochastic machine learning procedures for evaluation of expert utilities as criteria for optimization.

  4. Effect of different cooking methods on nutritional value and antioxidant activity of cultivated mushrooms.

    Science.gov (United States)

    Roncero-Ramos, Irene; Mendiola-Lanao, Mónica; Pérez-Clavijo, Margarita; Delgado-Andrade, Cristina

    2017-05-01

    Influence of culinary treatments (boiling, microwaving, grilling, and deep frying) on proximate composition and antioxidant capacity of cultivated mushrooms (Agaricus bisporus, Lentinula edodes, Pleurotus ostreatus, and Pleurotus eryngii) was studied. Proximate composition was affected by the cooking method and the mushrooms species. Frying induced more severe losses in protein, ash, and carbohydrates content but increased the fat and energy. Boiling improved the total glucans content by enhancing the β-glucans fraction. A significant decrease was detected in the antioxidant activity especially after boiling and frying, while grilled and microwaved mushrooms reached higher values of antioxidant activity. Maillard reaction products could be partially responsible, as supported by the absorbance values measured at 420 nm. Since cooking techniques clearly influence the nutritional attributes of mushrooms, the proper selection of treatments is a key factor to prevent/reduce nutritional losses. Microwaving and grilling were established as the best processes to maintain the nutritional profile of mushrooms.

  5. Analysis of yield of eleutherosides B and E in Acanthopanax divaricatus and A. koreanum grown with varying cultivation methods.

    Science.gov (United States)

    Lee, Jeong Min; Lee, Myoung-Hee; Pae, Suk-Bok; Oh, Ki-Won; Jung, Chan-Sik; Baek, In-Youl; Lee, Sanghyun

    2014-01-01

    An analysis of the yield of eleutherosides B and E in Acanthopanax divaricatus and A. koreanum was performed using high performance liquid chromatography to evaluate production by different cultivation methods. In A. divaricatus and A. koreanum, the total content of eleutherosides B and E was 2.466-7.360 mg/g varying by plant section, 3.886-11.506 mg/g by pinching site, 3.655-10.083 mg/g by planting time, and 3.652-10.108 mg/g by fertilizer ratio. Thus the total content of eleutherosides B and E in A. divaricatus and A. koreanum differed depending on cultivation methods. These results present useful information for high eleutheroside content applications in A. divaricatus and A. koreanum. This information can affect selection of plant section and cultivation methods for nutraceutical, pharmaceutical, and cosmeceutical material development.

  6. Analysis of Yield of Eleutherosides B and E in Acanthopanax divaricatus and A. koreanum Grown with Varying Cultivation Methods

    Directory of Open Access Journals (Sweden)

    Jeong Min Lee

    2014-01-01

    Full Text Available An analysis of the yield of eleutherosides B and E in Acanthopanax divaricatus and A. koreanum was performed using high performance liquid chromatography to evaluate production by different cultivation methods. In A. divaricatus and A. koreanum, the total content of eleutherosides B and E was 2.466–7.360 mg/g varying by plant section, 3.886–11.506 mg/g by pinching site, 3.655–10.083 mg/g by planting time, and 3.652–10.108 mg/g by fertilizer ratio. Thus the total content of eleutherosides B and E in A. divaricatus and A. koreanum differed depending on cultivation methods. These results present useful information for high eleutheroside content applications in A. divaricatus and A. koreanum. This information can affect selection of plant section and cultivation methods for nutraceutical, pharmaceutical, and cosmeceutical material development.

  7. Classification of cultivated plants.

    NARCIS (Netherlands)

    Brandenburg, W.A.

    1986-01-01

    Agricultural practice demands principles for classification, starting from the basal entity in cultivated plants: the cultivar. In establishing biosystematic relationships between wild, weedy and cultivated plants, the species concept needs re-examination. Combining of botanic classification, based

  8. Wild helianthus species used for broadening the genetic base of cultivated sunflower in India

    OpenAIRE

    Sujatha M

    2006-01-01

    The present investigation has been undertaken to introgress desirable traits from wild sunflowers to cultivated sunflower. Using conventional methods of crossing, backcrossing and selection, several pre-bred lines with altered plant architecture, high yield and oil content, maturity duration and inbuilt tolerance to major biotic stresses have been developed from crosses involving diploid annuals. These recombinant interspecific inbred lines are being utilized in the national sunflower network...

  9. Efficient animal-serum free 3D cultivation method for adult human neural crest-derived stem cell therapeutics

    Directory of Open Access Journals (Sweden)

    JFW Greiner

    2011-12-01

    Full Text Available Due to their broad differentiation potential and their persistence into adulthood, human neural crest-derived stem cells (NCSCs harbour great potential for autologous cellular therapies, which include the treatment of neurodegenerative diseases and replacement of complex tissues containing various cell types, as in the case of musculoskeletal injuries. The use of serum-free approaches often results in insufficient proliferation of stem cells and foetal calf serum implicates the use of xenogenic medium components. Thus, there is much need for alternative cultivation strategies. In this study we describe for the first time a novel, human blood plasma based semi-solid medium for cultivation of human NCSCs. We cultivated human neural crest-derived inferior turbinate stem cells (ITSCs within a blood plasma matrix, where they revealed higher proliferation rates compared to a standard serum-free approach. Three-dimensionality of the matrix was investigated using helium ion microscopy. ITSCs grew within the matrix as revealed by laser scanning microscopy. Genetic stability and maintenance of stemness characteristics were assured in 3D cultivated ITSCs, as demonstrated by unchanged expression profile and the capability for self-renewal. ITSCs pre-cultivated in the 3D matrix differentiated efficiently into ectodermal and mesodermal cell types, particularly including osteogenic cell types. Furthermore, ITSCs cultivated as described here could be easily infected with lentiviruses directly in substrate for potential tracing or gene therapeutic approaches. Taken together, the use of human blood plasma as an additive for a completely defined medium points towards a personalisable and autologous cultivation of human neural crest-derived stem cells under clinical grade conditions.

  10. A Comparative Accuracy Analysis of Classification Methods in Determination of Cultivated Lands with Spot 5 Satellite Imagery

    Science.gov (United States)

    kaya, S.; Alganci, U.; Sertel, E.; Ustundag, B.

    2013-12-01

    A Comparative Accuracy Analysis of Classification Methods in Determination of Cultivated Lands with Spot 5 Satellite Imagery Ugur ALGANCI1, Sinasi KAYA1,2, Elif SERTEL1,2,Berk USTUNDAG3 1 ITU, Center for Satellite Communication and Remote Sensing, 34469, Maslak-Istanbul,Turkey 2 ITU, Department of Geomatics, 34469, Maslak-Istanbul, Turkey 3 ITU, Agricultural and Environmental Informatics Research Center,34469, Maslak-Istanbul,Turkey alganci@itu.edu.tr, kayasina@itu.edu.tr, sertele@itu.edu.tr, berk@berk.tc ABSTRACT Cultivated land determination and their area estimation are important tasks for agricultural management. Derived information is mostly used in agricultural policies and precision agriculture, in specifically; yield estimation, irrigation and fertilization management and farmers declaration verification etc. The use of satellite image in crop type identification and area estimate is common for two decades due to its capability of monitoring large areas, rapid data acquisition and spectral response to crop properties. With launch of high and very high spatial resolution optical satellites in the last decade, such kind of analysis have gained importance as they provide information at big scale. With increasing spatial resolution of satellite images, image classification methods to derive the information form them have become important with increase of the spectral heterogeneity within land objects. In this research, pixel based classification with maximum likelihood algorithm and object based classification with nearest neighbor algorithm were applied to 2012 dated 2.5 m resolution SPOT 5 satellite images in order to investigate the accuracy of these methods in determination of cotton and corn planted lands and their area estimation. Study area was selected in Sanliurfa Province located on Southeastern Turkey that contributes to Turkey's agricultural production in a major way. Classification results were compared in terms of crop type identification using

  11. A supervision and control tool based on artificial intelligence for high cell density cultivations

    Directory of Open Access Journals (Sweden)

    A. C. L. Horta

    2014-06-01

    Full Text Available High cell density cultivations of recombinant E. coli have been increasingly used for the production of heterologous proteins. However, it is a challenge to maintain these cultivations within the desired conditions, given that some variables such as dissolved oxygen concentration (DOC and feed flow rate are difficult to control. This paper describes the software SUPERSYS_HCDC, a tool developed to supervise fed-batch cultures of rE. coli with biomass concentrations up to 150 gDCW/L and cell productivities up to 9 gDCW.L-1.h-1. The tool includes automatic control of the DOC by integrated action of the stirrer speed as well as of the air and oxygen flow rates; automatic start-up of the feed flow of fresh medium (system based on a neural network committee; and automatic slowdown of feeding when oxygen consumption exceeds the maximum capacity of the oxygen supply.

  12. Anaerobic High-Throughput Cultivation Method for Isolation of Thermophiles Using Biomass-Derived Substrates

    Energy Technology Data Exchange (ETDEWEB)

    Hamilton-Brehm, Scott [ORNL; Vishnivetskaya, Tatiana A [ORNL; Allman, Steve L [ORNL; Mielenz, Jonathan R [ORNL; Elkins, James G [ORNL

    2012-01-01

    Flow cytometry (FCM) techniques have been developed for sorting mesophilic organisms, but the difficulty increases if the target microbes are thermophilic anaerobes. We demonstrate a reliable, high-throughput method of screening thermophilic anaerobic organisms using FCM and 96-well plates for growth on biomass-relevant substrates. The method was tested using the cellulolytic thermophiles Clostridium ther- mocellum (Topt = 55 C), Caldicellulosiruptor obsidiansis (Topt = 78 C) and the fermentative hyperthermo- philes, Pyrococcus furiosus (Topt = 100 C) and Thermotoga maritima (Topt = 80 C). Multi-well plates were incubated at various temperatures for approximately 72 120 h and then tested for growth. Positive growth resulting from single cells sorted into individual wells containing an anaerobic medium was verified by OD600. Depending on the growth substrate, up to 80 % of the wells contained viable cultures, which could be transferred to fresh media. This method was used to isolate thermophilic microbes from Rabbit Creek, Yellowstone National Park (YNP), Wyoming. Substrates for enrichment cultures including crystalline cellulose (Avicel), xylan (from Birchwood), pretreated switchgrass and Populus were used to cultivate organisms that may be of interest to lignocellulosic biofuel production.

  13. Soil properties and crop yield under different tillage methods for rapeseed cultivation in paddy fields

    Directory of Open Access Journals (Sweden)

    Alizadeh Mohammad Reza

    2015-01-01

    Full Text Available A two-year research was conducted to investigate the effect of different tillage methods on some soil physical characteristics and crop yield in rapeseed cultivation after rice harvesting. Five tillage treatments including: (i using rotavator, once to depth of 10-15 cm (T1, (ii using rotavator, twice to depth of 10-15 cm (T2, (iii using moldboard plow to depth of 25 cm + rotavator, once to depth of 10-15 cm (T3, (iv no-till planting through removing rice stubbles from plots (T4, and (v no-till planting without removing rice stubbles from plots (T5, were evaluated under randomized complete block design (RCBD in three replications. The biannual results revealed that the effect of tillage methods was significant (p<0.01 on soil bulk density, surface residues after tillage, dry mass of weeds, seed germination, and grain yield. T2 and T3 made considerable reduction in soil bulk density compared to other treatments for the 15- to 30-cm tillage depths. In T1, T2, T3, and T4, surface residues after tillage decreased in comparison with T5 by up to 35.37, 50.71, 69.92, and 75.75%, respectively. Having 71.48 g m-2, T5 had the maximum dry mass of weeds while T3 had the minimum one with 37.50 g m-2. Means comparison represented that in T2 and T3, seed germination reached the shortest length of 6.4 days in average. The highest and lowest grain yields were acquired in T3 (1,571 kg ha-1 and T5 (1,339 kg ha-1, respectively. Statistically, there was no significant difference between T1 (1,432 kg ha-1 and T2 (1,537 kg ha-1 compared with T3 in terms of grain yield.

  14. Investigation of mineral water springs of Miercurea Ciuc (Csíkszereda) region (Romania) with cultivation-dependent microbiological methods.

    Science.gov (United States)

    Máthé, I; Táncsics, A; György, Eva; Pohner, Zsuzsanna; Vladár, P; Székely, Anna J; Márialigeti, K

    2010-06-01

    Water samples of ten mineral water springs at Miercurea Ciuc (Csíkszereda) region (Romania) were examined during 2005-2006 using cultivation-dependent microbiological methods. The results of standard hygienic bacteriological tests showed that the Hargita Spring had perfect and five other springs had microbiologically acceptable water quality (Zsögöd-, Nagy-borvíz-, Taploca-, Szentegyháza- and Lobogó springs). The water of Borsáros Spring was exceptionable (high germ count, presence of Enterococcus spp.).Both standard bacteriological and molecular microbiological methods indicated that the microbiological water quality of the Szeltersz-, Nádasszék- and Délo springs was not acceptable. Bad water quality resulted from inadequate spring catchment and hygiene (low yield, lack of runoff, negligent usage of the springs, horse manure around the spring).The 16S rRNA gene-based identification of strains isolated on standard meat-peptone medium resulted in the detection of typical aquatic organisms such as Shewanella baltica, Aeromonas spp., Pseudomonas veronii, Psychrobacter sp,. Acinetobacter spp. and allochthonous microbes, like Nocardia, Streptomyces, Bacillus, Microbacterium , and Arthrobacter strains indicating the impact of soil. Other allochthonous microbes, such as Staphylococcus spp., Micrococcus sp., Lactococcus sp., Clostridium butyricum, Yersinia spp., Aerococcus sp., may have originated from animal/human sources.

  15. Can agricultural Cultivation Methods Influence the Healthfulness of Crops for Foods?

    DEFF Research Database (Denmark)

    Melballe Jensen, Maja; Jørgensen, Henry; Halekoh, Ulrich

    2012-01-01

    . Additionally, the nutritional quality was affected by harvest year and location. However, harvest year and location rather than cultivation system affected the measured health biomarkers. In conclusion, the differences in dietary treatments composed of ingredients from different cultivation systems did......The aim of the current study was to investigate if there are any health effects of long-term consumption of organically grown crops using a rat model. Crops were retrieved over two years from a long-term field trial at three different locations in Denmark, using three different cultivation systems...... not lead to significant differences in the measured health biomarkers, except for a significant difference in plasma IgG levels....

  16. Secretome data from Trichoderma reesei and Aspergillus niger cultivated in submerged and sequential fermentation methods

    Directory of Open Access Journals (Sweden)

    Camila Florencio

    2016-09-01

    Full Text Available The cultivation procedure and the fungal strain applied for enzyme production may influence levels and profile of the proteins produced. The proteomic analysis data presented here provide critical information to compare proteins secreted by Trichoderma reesei and Aspergillus niger when cultivated through submerged and sequential fermentation processes, using steam-explosion sugarcane bagasse as inducer for enzyme production. The proteins were organized according to the families described in CAZy database as cellulases, hemicellulases, proteases/peptidases, cell-wall-protein, lipases, others (catalase, esterase, etc., glycoside hydrolases families, predicted and hypothetical proteins. Further detailed analysis of this data is provided in “Secretome analysis of Trichoderma reesei and Aspergillus niger cultivated by submerged and sequential fermentation process: enzyme production for sugarcane bagasse hydrolysis” C. Florencio, F.M. Cunha, A.C Badino, C.S. Farinas, E. Ximenes, M.R. Ladisch (2016 [1].

  17. Improved poliovirus d-antigen yields by application of different Vero cell cultivation methods

    OpenAIRE

    Thomassen, Y.E.; Rubingh, O.; Wijffels, R.H.; Pol, van de, F.C.M.

    2014-01-01

    Vero cells were grown adherent to microcarriers (Cytodex 1; 3 g L-1) using animal component free media in stirred-tank type bioreactors. Different strategies for media refreshment, daily media replacement (semi-batch), continuous media replacement (perfusion) and recirculation of media, were compared with batch cultivation. Cell densities increased using a feed strategy from 1 × 106 cells mL-1 during batch cultivation to 1.8, 2.7 and 5.0 × 106 cells mL-1 during semi-batch, perfusion and recir...

  18. Molecular identification based on ITS sequences for Kappaphycus and Eucheuma cultivated in China

    Science.gov (United States)

    Zhao, Sufen; He, Peimin

    2011-11-01

    The systematic classification of the Eucheumatoideae is difficult because of their variable morphology and interpretation of reproductive structures. Kappaphycus and Eucheuma specimens cultivated on the Hainan and Fujian coast of China were introduced from Vietnam, the Philippines and Indonesia. Combined with morphological characteristics, all Kappaphycus and Eucheuma cultivated strains were identified by internal transcribed spacer (ITS) sequences. The phylogenetic tree was constructed using neighbor-joining and maximum likelihood methods. The results indicate that different ITS sequence lengths occurred in the different genera and species. An obvious difference in morphology could be found in the protuberance shape between Kappaphycus and Eucheuma. The protuberance in Eucheuma was thorn-like and in Kappaphycus was wartlike or papillate. Their ITS sequence lengths differed significantly in nucleotide variation rates up to 58.55%-63.90%. All nucleotide variations occurred in the ITS1 and ITS2 regions except for five nucleotide transversions in the 5.8S rDNA region. In addition, the difference was at the branches among congeneric species. Kappaphycus sp. had branches with small buds, while K. alvarezii did not have such a feature. The nucleotide variation rates varied from 7.02% to 7.48% among species; within the same species of the clades it was <1.20%. Eucheumatoideae algae cultivated in China consisted of three clades, K. alvarezii, Kappaphycus sp., and E. denticulatum. The results indicate that ITS sequence analysis was an effective way for identification of interspecies and intraspecies phylogenetic relationships and might provide a clue for molecular identification of algal Eucheumatoideae.

  19. Molecular identification based on ITS sequences for Kappaphycus and Eucheuma cultivated in China

    Institute of Scientific and Technical Information of China (English)

    ZHAO Sufen; HE Peimin

    2011-01-01

    The systematic classification of the Eucheurnatoideae is difficult because of their variable morphology and interpretation of reproductive structures.Kappaphycus and Eucheuma specimens cultivated on the Hainan and Fujian coast of China were introduced from Vietnam,the Philippines and Indonesia.Combined with morphological characteristics,all Kappaphycus and Eucheuma cultivated strains were identified by internal transcribed spacer (ITS) sequences.The phylogenetic tree was constructed using neighbor-joining and maximum likelihood methods.The results indicate that different ITS sequence lengths occurred in the different genera and species.An obvious difference in morphology could be found in the protuberance shape between Kappaphycus and Eucheuma.The protuberance in Eucheuma was thorn-like and in Kappaphycus was wartlike or papillate.Their ITS sequence lengths differed significantly in nucleotide variation rates up to 58.55%-63.90%.All nucleotide variations occurred in the ITS1 andITS2 regions except for five nucleotide transversions in the 5.8S rDNA region.In addition,the difference was at the branches among congeneric species.Kappaphycus sp.had branches with small buds,while K.alvarezii did not have such a feature.The nucleotide variation rates varied from 7.02% to 7.48% among species; within the same species of the clades it was <1.20%.Eucheumatoideae algae cultivated in China consisted of three clades,K.alvarezii,Kappaphycus sp.,and E.denticulatum.The results indicate that ITS sequence analysis was an effective way for identification of interspecies and intraspecies phylogenetic relationships and might provide a clue for molecular identification of algal Eucheumatoideae.

  20. Comparison of phenotype of gammadelta T cells generated using various cultivation methods.

    Science.gov (United States)

    Mehrle, Stefan; Watzl, Carsten; von Lilienfeld-Toal, Marie; Amoroso, Alfredo; Schmidt, Jan; Märten, Angela

    2009-06-30

    It has been demonstrated, that gammadelta T cells play an important role in the development of immune responses to many pathogens. gammadelta T cells play a role in the clearance of viral and microbiological infections, anti-tumor responses, but also in autoimmune diseases. Many different protocols for the isolation and cultivation of gammadelta T cells can be found in the literature. Here we compare three common cultivation protocols for gammadelta T cells derived from peripheral blood with a newly developed protocol depending on SLAM (Signaling Lymphocyte Activation Molecule) stimulation. We demonstrate that the cultivation protocol chosen to raise gammadelta T cells has direct impact on the resulting gammadelta T cell phenotype. We show differences in gammadelta TCR composition, memory phenotype formation, CD8 receptor expression and the expression of NK cell markers depending on the stimulation protocol used. As such, the cultivation protocol chosen for a series of experiments might have significant impact on the outcome of the experiments and should be considered carefully.

  1. Cultivable endophytic bacteria from leaf bases of Agave tequilana and their role as plant growth promoters

    Science.gov (United States)

    Martínez-Rodríguez, Julia del C.; la Mora-Amutio, Marcela De; Plascencia-Correa, Luis A.; Audelo-Regalado, Esmeralda; Guardado, Francisco R.; Hernández-Sánchez, Elías; Peña-Ramírez, Yuri J.; Escalante, Adelfo; Beltrán-García, Miguel J.; Ogura, Tetsuya

    2014-01-01

    Agave tequilana Weber var. ‘Azul’ is grown for the production of tequila, inulin and syrup. Diverse bacteria inhabit plant tissues and play a crucial role for plant health and growth. In this study culturable endophytic bacteria were extracted from leaf bases of 100 healthy Agave tequilana plants. In plant tissue bacteria occurred at mean population densities of 3 million CFU/g of fresh plant tissue. Three hundred endophytic strains were isolated and 16s rDNA sequences grouped the bacteria into eight different taxa that shared high homology with other known sequences. Bacterial endophytes were identified as Acinectobacter sp., A. baumanii, A. bereziniae, Cronobacter sakazakii, Enterobacter hormaechei, Bacillus sp. Klebsiella oxytoca, Pseudomonas sp., Enterococcus casseliflavus, Leuconostoc mesenteroides subsp. mesenteroides and Gluconobacter oxydans. Isolates were confirmed to be plant growth promoting bacteria (PGPB) by their capacities for nitrogen fixation, auxin production, phosphate solubilization, or antagonism against Fusarium oxysporum AC132. E. casseliflavus JM47 and K. oxytoca JM26 secreted the highest concentrations of IAA. The endophyte Acinectobacter sp. JM58 exhibited the maximum values for nitrogen fixation and phosphate solubilization index (PSI). Inhibition of fungi was found in Pseudomonas sp. JM9p and K. oxytoca JM26. Bacterial endophytes show promise for use as bio-inoculants for agave cultivation. Use of endophytes to enhance cultivation of agave may be particularly important for plants produced by micropropagation techniques, where native endophytes may have been lost. PMID:25763038

  2. Cultivable endophytic bacteria from leaf bases of Agave tequilana and their role as plant growth promoters.

    Science.gov (United States)

    Martínez-Rodríguez, Julia del C; De la Mora-Amutio, Marcela; Plascencia-Correa, Luis A; Audelo-Regalado, Esmeralda; Guardado, Francisco R; Hernández-Sánchez, Elías; Peña-Ramírez, Yuri J; Escalante, Adelfo; Beltrán-García, Miguel J; Ogura, Tetsuya

    2014-01-01

    Agave tequilana Weber var. 'Azul' is grown for the production of tequila, inulin and syrup. Diverse bacteria inhabit plant tissues and play a crucial role for plant health and growth. In this study culturable endophytic bacteria were extracted from leaf bases of 100 healthy Agave tequilana plants. In plant tissue bacteria occurred at mean population densities of 3 million CFU/g of fresh plant tissue. Three hundred endophytic strains were isolated and 16s rDNA sequences grouped the bacteria into eight different taxa that shared high homology with other known sequences. Bacterial endophytes were identified as Acinectobacter sp., A. baumanii, A. bereziniae, Cronobacter sakazakii, Enterobacter hormaechei, Bacillus sp. Klebsiella oxytoca, Pseudomonas sp., Enterococcus casseliflavus, Leuconostoc mesenteroides subsp. mesenteroides and Gluconobacter oxydans. Isolates were confirmed to be plant growth promoting bacteria (PGPB) by their capacities for nitrogen fixation, auxin production, phosphate solubilization, or antagonism against Fusarium oxysporum AC132. E. casseliflavus JM47 and K. oxytoca JM26 secreted the highest concentrations of IAA. The endophyte Acinectobacter sp. JM58 exhibited the maximum values for nitrogen fixation and phosphate solubilization index (PSI). Inhibition of fungi was found in Pseudomonas sp. JM9p and K. oxytoca JM26. Bacterial endophytes show promise for use as bio-inoculants for agave cultivation. Use of endophytes to enhance cultivation of agave may be particularly important for plants produced by micropropagation techniques, where native endophytes may have been lost.

  3. Cultivable endophytic bacteria from leaf bases of Agave tequilana and their role as plant growth promoters

    Directory of Open Access Journals (Sweden)

    Julia del C. Martínez-Rodríguez

    2014-12-01

    Full Text Available Agave tequilana Weber var. 'Azul' is grown for the production of tequila, inulin and syrup. Diverse bacteria inhabit plant tissues and play a crucial role for plant health and growth. In this study culturable endophytic bacteria were extracted from leaf bases of 100 healthy Agave tequilana plants. In plant tissue bacteria occurred at mean population densities of 3 million CFU/g of fresh plant tissue. Three hundred endophytic strains were isolated and 16s rDNA sequences grouped the bacteria into eight different taxa that shared high homology with other known sequences. Bacterial endophytes were identified as Acinectobacter sp., A. baumanii, A. bereziniae, Cronobacter sakazakii, Enterobacter hormaechei, Bacillus sp. Klebsiella oxytoca, Pseudomonas sp., Enterococcus casseliflavus, Leuconostoc mesenteroides subsp. mesenteroides and Gluconobacter oxydans. Isolates were confirmed to be plant growth promoting bacteria (PGPB by their capacities for nitrogen fixation, auxin production, phosphate solubilization, or antagonism against Fusarium oxysporum AC132. E. casseliflavus JM47 and K. oxytoca JM26 secreted the highest concentrations of IAA. The endophyte Acinectobacter sp. JM58 exhibited the maximum values for nitrogen fixation and phosphate solubilization index (PSI. Inhibition of fungi was found in Pseudomonas sp. JM9p and K. oxytoca JM26. Bacterial endophytes show promise for use as bio-inoculants for agave cultivation. Use of endophytes to enhance cultivation of agave may be particularly important for plants produced by micropropagation techniques, where native endophytes may have been lost.

  4. Possible Influence of the Cultivated Land Reclamation on Surface Climate in India: A WRF Model Based Simulation

    Directory of Open Access Journals (Sweden)

    Yi Qu

    2013-01-01

    Full Text Available Land use/cover change (LUCC has become one of the most important factors for the global climate change. As one of the major types of LUCC, cultivated land reclamation also has impacts on regional climate change. Most of the previous studies focused on the correlation and simulation analysis of historical LUCC and climate change, with few explorations for the impacts of future LUCC on regional climate, especially impacts of the cultivated land reclamation. This study used the Weather Research and Forecasting (WRF model to forecast the changes of energy flux and temperature based on the future cultivated land reclamation in India and then analyzed the impacts of cultivated land reclamation on climate change. The results show that cultivated land reclamation will lead to a large amount of land conversions, which will overall result in the increase in latent heat flux of regional surface as well as the decrease in sensible heat flux and further lead to changes of regional average temperature. Furthermore, the impact on climate change is seasonally different. The cultivated land reclamation mainly leads to a temperature decrease in the summer, while it leads to a temperature increase in the winter.

  5. Carotenoid profiling from 27 types of paprika (Capsicum annuum L.) with different colors, shapes, and cultivation methods.

    Science.gov (United States)

    Kim, Ji-Sun; An, Chul Geon; Park, Jong-Suk; Lim, Yong Pyo; Kim, Suna

    2016-06-15

    In this study, we investigated carotenoid profiles and contents from 27 types of paprika with different colors (red, orange, and yellow), shapes (blocky and conical), and cultivation methods (soil and soilless). We simultaneously analyzed 12 kinds of carotenoids using UPLC equipped with an HSS T3 column for 30 min, and we identified six kinds of carotenoids in red paprika and nine types in orange and yellow paprika. Zeaxanthin concentrations in orange paprika were in the range of 85.06±23.37-151.39±5.94 mg/100 g dry weight (dw), which shows that orange paprika is a great source of zeaxanthin. Generally, red paprika is a great source of capsanthin. However, a new cultivar, 'Mini Goggal Red', contained large amounts of zeaxanthin (121.41±30.10 mg/100 g dw) even though its visible color is red. This is very meaningful considering that consumers have a preference for red color and the potent functional value of zeaxanthin. Carotenoid profiles and concentrations in blocky and conical type paprika were not significantly different in red paprika except the 'Mini Goggal Red' cultivar and yellow paprika. Blocky type orange paprika contains plenty of zeaxanthin, unlike conical type orange paprika. Three new cultivars of the conical type were cultivated in both soil culture and soilless culture in the same province, and carotenoid profiles and concentrations were similar, showing that both cultivations methods can be used.

  6. Comparison of Cheng's Index-and SSR Marker-based Classification of Asian Cultivated Rice

    Institute of Scientific and Technical Information of China (English)

    WANG Cai-hong; XU Qun; YU Ping; YUAN Xiao-ping; YU Han-yong; WANG Yi-ping; TANG Sheng-xiang

    2013-01-01

    A total of 100 cultivated rice accessions,with a clear isozyme-based classification,were analyzed based on Cheng's index and simple sequence repeat (SSR) marker.The results showed that the isozyme-based classification was in high accordance with that based on Cheng's index and SSR markers.Mantel-test revealed that the Euclidean distance of Cheng's index was significantly correlated with Nei's unbiased genetic distance of SSR markers (r =0.466,P ≤ 0.01).According to the model-based group and cluster analysis,the Cheng's index-and SSR-based classification coincided with each other,with the goodness of fit of 82.1% and 84.7% in indica,97.4% and 95.1% in japonica,respectively,showing higher accordance than that within subspecies.Therefore,Cheng's index could be used to classify subspecies,while SSR marker could be more efficient to analyze the subgroups within subspecies.

  7. Cultivation of Marine Sponges.

    Science.gov (United States)

    Osinga; Tramper; Wijffels

    1999-11-01

    There is increasing interest in biotechnological production of marine sponge biomass owing to the discovery of many commercially important secondary metabolites in this group of animals. In this article, different approaches to producing sponge biomass are reviewed, and several factors that possibly influence culture success are evaluated. In situ sponge aquacultures, based on old methods for producing commercial bath sponges, are still the easiest and least expensive way to obtain sponge biomass in bulk. However, success of cultivation with this method strongly depends on the unpredictable and often suboptimal natural environment. Hence, a better-defined production system would be desirable. Some progress has been made with culturing sponges in semicontrolled systems, but these still use unfiltered natural seawater. Cultivation of sponges under completely controlled conditions has remained a problem. When designing an in vitro cultivation method, it is important to determine both qualitatively and quantitatively the nutritional demands of the species that is to be cultured. An adequate supply of food seems to be the key to successful sponge culture. Recently, some progress has been made with sponge cell cultures. The advantage of cell cultures is that they are completely controlled and can easily be manipulated for optimal production of the target metabolites. However, this technique is still in its infancy: a continuous cell line has yet to be established. Axenic cultures of sponge aggregates (primmorphs) may provide an alternative to cell culture. Some sponge metabolites are, in fact, produced by endosymbiotic bacteria or algae that live in the sponge tissue. Only a few of these endosymbionts have been cultivated so far. The biotechnology for the production of sponge metabolites needs further development. Research efforts should be continued to enable commercial exploitation of this valuable natural resource in the near future.

  8. Biodegradation and Osteosarcoma Cell Cultivation on Poly(aspartic acid) Based Hydrogels.

    Science.gov (United States)

    Juriga, Dávid; Nagy, Krisztina; Jedlovszky-Hajdú, Angéla; Perczel-Kovách, Katalin; Chen, Yong Mei; Varga, Gábor; Zrínyi, Miklós

    2016-09-14

    Development of novel biodegradable and biocompatible scaffold materials with optimal characteristics is important for both preclinical and clinical applications. The aim of the present study was to analyze the biodegradability of poly(aspartic acid)-based hydrogels, and to test their usability as scaffolds for MG-63 osteoblast-like cells. Poly(aspartic acid) was fabricated from poly(succinimide) and hydrogels were prepared using natural amines as cross-linkers (diaminobutane and cystamine). Disulfide bridges were cleaved to thiol groups and the polymer backbone was further modified with RGD sequence. Biodegradability of the hydrogels was evaluated by experiments on the base of enzymes and cell culture medium. Poly(aspartic acid) hydrogels possessing only disulfide bridges as cross-links proved to be degradable by collagenase I. The MG-63 cells showed healthy, fibroblast-like morphology on the double cross-linked and RGD modified hydrogels. Thiolated poly(aspartic acid) based hydrogels provide ideal conditions for adhesion, survival, proliferation, and migration of osteoblast-like cells. The highest viability was found on the thiolated PASP gels while the RGD motif had influence on compacted cluster formation of the cells. These biodegradable and biocompatible poly(aspartic acid)-based hydrogels are promising scaffolds for cell cultivation.

  9. 基于双边界二分式条件价值法的农户耕地补偿意愿评估%An evaluation of farmers’ willingness for compensation for cultivated land based on the double-bounded dichotomous contingent valuation method

    Institute of Scientific and Technical Information of China (English)

    马爱慧

    2015-01-01

    条件价值法是目前资源环境经济价值货币评估中较为广泛的应用方法,其经济理论基础虽已趋完善,但准确性仍受质疑,而双边界二分式条件价值法是可以最大限度地减少偏差的支付诱导方式。本文将双边界二分式CVM模型引入农民对耕地资源保护补偿意愿的经济价值评估研究中,为平衡同一主体受偿意愿与支付意愿存在较大的差异性,基于不同的假想市场,以武汉市450份农村居民意愿为例,进行农户对耕地资源保护的接受意愿和支付意愿的实证分析,以探索条件价值评估法双边界二分式意愿引导技术在我国应用的适用性。研究表明:农户的受偿意愿每亩在309.30~650.39元之间,其点估计值为477.47元;支付意愿在206.87~380.38元之间,其点估计值为290.86元。根据我国国情、补偿时空差异性和实施的可操作性,确定具有一定弹性的耕地资源保护补偿意愿标准为每亩290.86~477.47元之间。%The contingent valuation method (CVM) is widely used to evaluate the economic value of environmental resources. Although the economic theory behind the CVM has improved, its accuracy has been questioned. The double-bounded dichotomous choice method can minimise the error induced through payment. Using the double-bounded dichotomous choice method, this study analyses farmers’ wilingness to be compensated for cultivated land compared to their wilingness to pay or accept. It constructs a different hypothetical market based on a questionnaire sent to 450 rural residents in Wuhan as an example and explores the application of the double-bounded dichotomous choice method in China. Research shows that farmers’ wilingness to accept compensation is 309.30–650.39 RMB per Mu (1hm2=15Mu ), with point estimates of 477.47 RMB; and their wilingness to pay is 206.87–380.38 RMB, with point estimates of 290.86 RMB. According to the situation in China regarding

  10. Analysis of Yield of Eleutherosides B and E in Acanthopanax divaricatus and A. koreanum Grown with Varying Cultivation Methods

    OpenAIRE

    Jeong Min Lee; Myoung-Hee Lee; Suk-Bok Pae; Ki-Won Oh; Chan-Sik Jung; In-Youl Baek; Sanghyun Lee

    2014-01-01

    An analysis of the yield of eleutherosides B and E in Acanthopanax divaricatus and A. koreanum was performed using high performance liquid chromatography to evaluate production by different cultivation methods. In A. divaricatus and A. koreanum, the total content of eleutherosides B and E was 2.466–7.360 mg/g varying by plant section, 3.886–11.506 mg/g by pinching site, 3.655–10.083 mg/g by planting time, and 3.652–10.108 mg/g by fertilizer ratio. Thus the total content of eleutherosides B an...

  11. Calculating and testing cultivated land protection externality using Contingent Valuation Method%基于CVM的耕地保护外部性估算与检验

    Institute of Scientific and Technical Information of China (English)

    牛海鹏; 王文龙; 张安录

    2014-01-01

    This paper proposed the Contingent Valuation Method (CVM) for estimating cultivated land protection externality. The proposed CVM was based on reconstruction of cultivated land utilization benefit system and defined cultivated land protection externality. The paper discussed in detail specific techniques of sample size and distribution, determination of leading technology, pay/compensation interval setting, hypothetical market scenario setting, evasion of deviation, pretreatment of survey data, and results validity/reliability tests. The results showed that ecological and social benefits of cultivated lands included public goods with mixed classes across categories. These classes were provided by joint ventures between the pure positive externalities and the private sector (mainly farmers). This could be estimated by using CVM and other non-market valuation methods. The willingness to pay of inter-viewee for this service was heavily restricted and influenced by their levels of socioeconomic development. The recognition of eco-logical and social benefits of cultivated lands of farmers and urban residents formed the main causality factor for the differences in payment ratio between urban residents and rural farmers. Urban residents were more familiar with ecological and social benefits of cultivated lands than rural/farm households. This was probably a key factor influencing the willingness to pay for ecological services. Farm households preferred voluntary labor as mean of payment, while both money and voluntary labor were more preferred by urban residents. The probable reason for this was that farm households had more leisure time than urban residents. Unitary cultivated land protection externality for Jiaozuo City was 1.91×108 Yuan·a−1 and the cultivated land protection externality per unit area of cultivated land was 993.7 Yuan·hm−2·a−1 base on CVM. CVM validity and reliability test was a critical element of CVM theory, method and practice. This was

  12. Research on Cultivation Mode of Engineering Practical Ability Based on Post Simulation – Taking the hydrology and water resources engineering major in Tianjin Agricultural University as the example

    Directory of Open Access Journals (Sweden)

    Wang Lizhen

    2015-01-01

    Full Text Available This article aims at exploring the cultivation mode of engineering practical abstract based on post simulation to provide scientific and reasonable basis for talent cultivation. According to survey of hydrology and water resources industry, we determine the talent specification and its post group, analyze the relationship of post group and course system, reform the teaching system and relevant condition and integrate the engineering practice of post simulation. Finally, we make an analysis and verification on the cultivation effect in different perspective and find that each ability of students cultivated from this mode has been greatly improved, by which we confirm that the cultivation mode of engineering practical ability based on post simulation has an important meaning for cultivating talents in engineering faculty.

  13. On-line gas analysis in animal cell cultivation: II. Methods for oxygen uptake rate estimation and its application to controlled feeding of glutamine.

    Science.gov (United States)

    Eyer, K; Oeggerli, A; Heinzle, E

    1995-01-05

    Different methods for oxygen uptake rate (OUR) determinations in animal cell cultivation were investigated using a high quality mass spectrometer. Dynamic measurements have considerable disadvantages because of disturbances of the growing cells by the necessary variations of dissolved oxygen concentration. Only infrequent discrete measurements are possible using this method. Stationary liquid phase balance yielded better results with much higher frequency. Gas phase balancing has the advantage of not requiring dissolved oxygen measurement and knowledge of K(L)a, both of them are easily biased. It was found that simple gas phase balancing is either very inaccurate (error larger than expected signal) or very slow, with gas phase residence times of several hours. Therefore, a new method of aeration was designed. Oxygen and CO(2) transfer are mainly achieved via sparging. The gas released to the headspace is diluted with a roughly 100-fold stream of an inert gas (helium). Through this dilution, gas ratios are not changed for O(2), CO(2), Ar, and N(2). The measurement of lower concentrations (parts per million and below) is easy using mass spectrometry with a secondary electron multiplier. With this new method an excellent accuracy and sufficient speed of analysis were obtained. All these on-line methods for OUR measurement were tested during the cultivation of animal cells. The new method allowed better study of the kinetics of animal cell cultures as was shown with a hybridoma cell line (HFN 7.1, ATCC CRL 1606) producing monoclonal antibodies against human fibronectin. With the aid of these methods it was possible to find a correlation between a rapid decrease in oxygen uptake rate (OUR) and glutamine concentration. The sudden decrease in OUR can be attributed to glutamine depletion. This provided a basis for the controlled addition of glutamine to reduce the formation of ammonia produced by hydrolysis. This control method based on OUR measurement resulted in

  14. A SSR-based composite genetic linkage map for the cultivated peanut (Arachis hypogaea L. genome

    Directory of Open Access Journals (Sweden)

    Li Shaoxiong

    2010-01-01

    Full Text Available Abstract Background The construction of genetic linkage maps for cultivated peanut (Arachis hypogaea L. has and continues to be an important research goal to facilitate quantitative trait locus (QTL analysis and gene tagging for use in a marker-assisted selection in breeding. Even though a few maps have been developed, they were constructed using diploid or interspecific tetraploid populations. The most recently published intra-specific map was constructed from the cross of cultivated peanuts, in which only 135 simple sequence repeat (SSR markers were sparsely populated in 22 linkage groups. The more detailed linkage map with sufficient markers is necessary to be feasible for QTL identification and marker-assisted selection. The objective of this study was to construct a genetic linkage map of cultivated peanut using simple sequence repeat (SSR markers derived primarily from peanut genomic sequences, expressed sequence tags (ESTs, and by "data mining" sequences released in GenBank. Results Three recombinant inbred lines (RILs populations were constructed from three crosses with one common female parental line Yueyou 13, a high yielding Spanish market type. The four parents were screened with 1044 primer pairs designed to amplify SSRs and 901 primer pairs produced clear PCR products. Of the 901 primer pairs, 146, 124 and 64 primer pairs (markers were polymorphic in these populations, respectively, and used in genotyping these RIL populations. Individual linkage maps were constructed from each of the three populations and a composite map based on 93 common loci were created using JoinMap. The composite linkage maps consist of 22 composite linkage groups (LG with 175 SSR markers (including 47 SSRs on the published AA genome maps, representing the 20 chromosomes of A. hypogaea. The total composite map length is 885.4 cM, with an average marker density of 5.8 cM. Segregation distortion in the 3 populations was 23.0%, 13.5% and 7.8% of the markers

  15. Development of test models to quantify encapsulated bioburden in spacecraft polymer materials by cultivation-dependent and molecular methods

    Science.gov (United States)

    Bauermeister, Anja; Moissl-Eichinger, Christine; Mahnert, Alexander; Probst, Alexander; Flier, Niwin; Auerbach, Anna; Weber, Christina; Haberer, Klaus; Boeker, Alexander

    Bioburden encapsulated in spacecraft polymers (such as adhesives and coatings) poses a potential risk to scientific exploration of other celestial bodies, but it is not easily detectable. In this study, we developed novel testing strategies to estimate the quantity of intrinsic encapsulated bioburden in polymers used frequently on spaceflight hardware. In particular Scotch-Weld (TM) 2216 B/A (Epoxy adhesive); MAP SG121FD (Silicone coating), Solithane (®) 113 (Urethane resin); ESP 495 (Silicone adhesive); and Dow Corning (®) 93-500 (Silicone encapsulant) were investigated. As extraction of bioburden from polymerized (solid) materials did not prove feasible, a method was devised to extract contaminants from uncured polymer precursors by dilution in organic solvents. Cultivation-dependent analyses showed less than 0.1-2.5 colony forming units (cfu) per cm³ polymer, whereas quantitative PCR with extracted DNA indicated considerably higher values, despite low DNA extraction efficiency. Results obtained by this method reflected the most conservative proxy for encapsulated bioburden. To observe the effect of physical and chemical stress occurring during polymerization on the viability of encapsulated contaminants, Bacillus safensis spores were embedded close to the surface in cured polymer, which facilitated access for different analytical techniques. Staining by AlexaFluor succinimidyl ester 488 (AF488), propidium monoazide (PMA), CTC (5-cyano-2,3-diotolyl tetrazolium chloride) and subsequent confocal laser scanning microscopy (CLSM) demonstrated that embedded spores retained integrity, germination and cultivation ability even after polymerization of the adhesive Scotch-Weld™ 2216 B/A.

  16. Production of intraspecific F1 hybrids between wild and cultivated accessions of cowpea (Vigna unguiculata (L.) walp.) using conventional methods.

    Science.gov (United States)

    Lelou, B; Van Damme, P

    2006-01-01

    Cowpea (Vigna unguiculata (L.) Walp.) is an important food legume in the tropics. It belongs to the Phaseoleae (L.) tribe (Fabaceae family), it is diploid and its chromosome number is 22. Its gene pool includes the cultivated cowpea and its wild relatives, which are connected with Vigna subgenus, Catiang section. Cowpea has a great potential in increasing food legume production. The cowpea varieties, however, are susceptible to a number of insect pests, especially the pod borer Maruca testulalis and a pod sucking-bug complex (e.g.: Clavigralla tomentosicollis, Anoplocnemis curvipes and Riptortus dentipes), which cause severe damage. The crossing programme presented here exploits the variability existing in the wild African germplasm of V. unguiculata and cultivated cowpea. To incorporate the insect pest resistance into the cultivated cowpea economically, reciprocal crosses between wild forms and cowpea varieties were performed, using the stigmatic pollination methods at anthesis. Some barriers were found in these intraspecific crosses. In the majority of reciprocal crosses, the growth of the pollen tubes was arrested in the stigmatic tissue. Only 16.01% of the ovules were fertilised. In these ovules, embryo development was normal at about 20-25 days after pollination. The failure of the intraspecific crosses in about 80.7% of the cases is thus the result of the lack of fertilisation and the unfertilised ovules. There seems to exist considerable incompatibility within the primary cowpea gene pool. The breeding programme carried out under controlled conditions has proved to be less successful in developed cowpea intraspecific F1 hybrids. Further studies should concentrate on germplasm from Africa with documented resistance to major insect pests. In addition, the application of techniques for bypassing barriers to hybridisation of parent genotypes should enable these embryos to grow to plants.

  17. A ddRAD Based Linkage Map of the Cultivated Strawberry, Fragaria xananassa.

    Science.gov (United States)

    Davik, Jahn; Sargent, Daniel James; Brurberg, May Bente; Lien, Sigbjørn; Kent, Matthew; Alsheikh, Muath

    2015-01-01

    The cultivated strawberry (Fragaria ×ananassa Duch.) is an allo-octoploid considered difficult to disentangle genetically due to its four relatively similar sub-genomic chromosome sets. This has been alleviated by the recent release of the strawberry IStraw90 whole genome genotyping array. However, array resolution relies on the genotypes used in the array construction and may be of limited general use. SNP detection based on reduced genomic sequencing approaches has the potential of providing better coverage in cases where the studied genotypes are only distantly related from the SNP array's construction foundation. Here we have used double digest restriction-associated DNA sequencing (ddRAD) to identify SNPs in a 145 seedling F1 hybrid population raised from the cross between the cultivars Sonata (♀) and Babette (♂). A linkage map containing 907 markers which spanned 1,581.5 cM across 31 linkage groups representing the 28 chromosomes of the species. Comparing the physical span of the SNP markers with the F. vesca genome sequence, the linkage groups resolved covered 79% of the estimated 830 Mb of the F. × ananassa genome. Here, we have developed the first linkage map for F. × ananassa using ddRAD and show that this technique and other related techniques are useful tools for linkage map development and downstream genetic studies in the octoploid strawberry.

  18. A ddRAD Based Linkage Map of the Cultivated Strawberry, Fragaria xananassa.

    Directory of Open Access Journals (Sweden)

    Jahn Davik

    Full Text Available The cultivated strawberry (Fragaria ×ananassa Duch. is an allo-octoploid considered difficult to disentangle genetically due to its four relatively similar sub-genomic chromosome sets. This has been alleviated by the recent release of the strawberry IStraw90 whole genome genotyping array. However, array resolution relies on the genotypes used in the array construction and may be of limited general use. SNP detection based on reduced genomic sequencing approaches has the potential of providing better coverage in cases where the studied genotypes are only distantly related from the SNP array's construction foundation. Here we have used double digest restriction-associated DNA sequencing (ddRAD to identify SNPs in a 145 seedling F1 hybrid population raised from the cross between the cultivars Sonata (♀ and Babette (♂. A linkage map containing 907 markers which spanned 1,581.5 cM across 31 linkage groups representing the 28 chromosomes of the species. Comparing the physical span of the SNP markers with the F. vesca genome sequence, the linkage groups resolved covered 79% of the estimated 830 Mb of the F. × ananassa genome. Here, we have developed the first linkage map for F. × ananassa using ddRAD and show that this technique and other related techniques are useful tools for linkage map development and downstream genetic studies in the octoploid strawberry.

  19. Ground-based studies of tropisms in hardware developed for the European Modular Cultivation System (EMCS)

    Science.gov (United States)

    Correll, Melanie J.; Edelmann, Richard E.; Hangarter, Roger P.; Mullen, Jack L.; Kiss, John Z.

    Phototropism and gravitropism play key roles in the oriented growth of roots in flowering plants. In blue or white light, roots exhibit negative phototropism, but red light induces positive phototropism in Arabidopsis roots. The blue-light response is controlled by the phototropins while the red-light response is mediated by the phytochrome family of photoreceptors. In order to better characterize root phototropism, we plan to perform experiments in microgravity so that this tropism can be more effectively studied without the interactions with the gravity response. Our experiments are to be performed on the European Modular Cultivation System (EMCS), which provides an incubator, lighting system, and high resolution video that are on a centrifuge palette. These experiments will be performed at μg, 1g (control) and fractional g-levels. In order to ensure success of this mission on the International Space Station, we have been conducting ground-based studies on growth, phototropism, and gravitropism in experimental unique equipment (EUE) that was designed for our experiments with Arabidopsis seedlings. Currently, the EMCS and our EUE are scheduled for launch on space shuttle mission STS-121. This project should provide insight into how the blue- and red-light signaling systems interact with each other and with the gravisensing system.

  20. Biosurfactant Production by Cultivation of Bacillus atrophaeus ATCC 9372 in Semidefined Glucose/Casein-Based Media

    Science.gov (United States)

    Das Neves, Luiz Carlos Martins; de Oliveira, Kátia Silva; Kobayashi, Márcio Junji; Vessoni Penna, Thereza Christina; Converti, Attilio

    Biosurfactants are proteins with detergent, emulsifier, and antimicrobial actions that have potential application in environmental applications such as the treatment of organic pollutants and oil recovery. Bacillus atrophaeus strains are nonpathogenic and are suitable source of biosurfactants, among which is surfactin. The aim of this work is to establish a culture medium composition able to stimulate biosurfactants production by B. atrophaeus ATCC 9372. Batch cultivations were carried out in a rotary shaker at 150 rpm and 35°C for 24 h on glucose- and/or casein-based semidefined culture media also containing sodium chloride, dibasic sodium phosphate, and soy flour. The addition of 14.0 g/L glucose in a culture medium containing 10.0 g/L of casein resulted in 17 times higher biosurfactant production (B max=635.0 mg/L). Besides, the simultaneous presence of digested casein (10.0 g/L), digested soy flour (3.0 g/L), and glucose (18.0 g/L) in the medium was responsible for a diauxic effect during cell growth. Once the diauxie started, the average biosurfactants concentration was 16.8% less than that observed before this phenomenon. The capability of B. atrophaeus strain to adapt its own metabolism to use several nutrients as energy sources and to preserve high levels of biosurfactants in the medium during the stationary phase is a promising feature for its possible application in biological treatments.

  1. Mechanized methods for harvesting residual biomass from Mediterranean fruit tree cultivations

    Directory of Open Access Journals (Sweden)

    Borja Velázquez-Martí

    2012-06-01

    Full Text Available This study evaluates the technology and work systems used in order to harvest residual biomass from pruning in the specific conditions of Mediterranean fruit orchards (narrow distances between crop-rows. Harvesting has been divided into several types of operations - pruning, biomass alignment between crop tracks, biomass concentration in piles, chipping and bundling - which have been analyzed in five Mediterranean cultivations for three years. Altogether, three types of pruning have been analyzed: Manual, previous mechanical followed by manual, and fully mechanical; Two types of alignment: Manual and mechanical; Three concentration systems: Manual, tractor with a rake and a forwarder; Four chipping work organization systems: chipper driven inside orchard and manually fed by operators, mobile chipper driven inside orchard with pick-up header, mobile chipper fed by means of mechanical crane, chipper mounted on a truck fed by means of mechanical crane, which was working in a fixed position in a border of the plot after wood concentration. Also two bundling organization systems were checked: bundler machine working in a fixed position after wood concentration and working inside the plot driven among the crops. Previous concentration of the materials was the best alternative for their chipping or bundling in the studied conditions. Regression models have been calculated to predict the time of work of machinery and labor for each alternative. These equations were used to implement logistic planning as the Borvemar model, which defines a logistics network for supplying bio-energy systems.

  2. Effects of site conditions and methods of cultivation on growth of sawtooth oak plantations

    Institute of Scientific and Technical Information of China (English)

    Luozhong TANG; Mukui YU; Dan ZHAO; Chunfeng YAN; Zhilong LIU; Shengzuo FANG

    2009-01-01

    The effects of site conditions and cultivation on the growth of sawtooth oak {Quercus acutissima Carr.) plantations were evaluated at the Hongyashan forest farm, in Chuzhou City, Anhui Province, China. The results indicate that the position on the slope, the amount of gravel and the thickness of the soil were important factors in the growth of the sawtooth oak. Lower slope positions with small amounts of gravel and a thick soil were better for the growth of this species than middle slope positions with more gravel and a thin soil. Given the site conditions of the hilly and mountainous areas in Chuzhou City, the mixed Chinese fir (Cunninghamia lanceolata Hook.) and sawtooth oak forests did not improve forest productivity compared with pure sawtooth oak forests. Both urea and compound fertilizers promoted the growth of sawtooth oak, as did site preparation and intercropping. Two years after planting, the height growth of ordinary seedlings with a starting height of 0.6 m was higher than that of supper seedlings with a starting height of 1.0 m. Compared with planting, the early growth of the coppices was faster, but the later growth of the coppices was slower.

  3. The influence of cultivation method on the flowering of Salvia horminum L. 'Oxford Blue'

    Directory of Open Access Journals (Sweden)

    Elżbieta Pogroszewska

    2012-12-01

    Full Text Available Salvia horminum L. 'Oxford Blue' was grown from transplants or it was direct seeded. Four sowing dates were applied: 15 March or 30 March - in a greenhouse, in order to obtain transplants, 13 April, 27 April and 11 May - sown directly into the ground. Three plant densities were applied: 25 plants×m2, 16 plants×m2, 12 plants×m2. It was found that Horminum sage can be grown from transplants or direct seeded. The cultivation from transplants is more advantageous due to the earlier flowering of plants, by about two weeks, and a better quality of inflorescences evaluated in terms of their length and size of bracteoles. Direct sowing of Horminum sage at two-week intervals from the 2nd decade of April till the 2nd decade of May ensures that by the end of August mature inflorescence stems are obtained, ready for cutting. A delay in the date of sowing results in the development of significantly shorter inflorescence stems, irrespective of the plant density. Plant density does not affect significantly the length of the period of inflorescence formation and the date of flowering, but a larger spacing is favourable to plants growing big, what results in a larger fresh weight of the above-ground portion. Sage grown from transplants responses to favourable climatic conditions by the development of bigger inflorescences.

  4. A simple method for decomposition of peracetic acid in a microalgal cultivation system.

    Science.gov (United States)

    Sung, Min-Gyu; Lee, Hansol; Nam, Kibok; Rexroth, Sascha; Rögner, Matthias; Kwon, Jong-Hee; Yang, Ji-Won

    2015-03-01

    A cost-efficient process devoid of several washing steps was developed, which is related to direct cultivation following the decomposition of the sterilizer. Peracetic acid (PAA) is known to be an efficient antimicrobial agent due to its high oxidizing potential. Sterilization by 2 mM PAA demands at least 1 h incubation time for an effective disinfection. Direct degradation of PAA was demonstrated by utilizing components in conventional algal medium. Consequently, ferric ion and pH buffer (HEPES) showed a synergetic effect for the decomposition of PAA within 6 h. On the contrary, NaNO3, one of the main components in algal media, inhibits the decomposition of PAA. The improved growth of Chlorella vulgaris and Synechocystis PCC6803 was observed in the prepared BG11 by decomposition of PAA. This process involving sterilization and decomposition of PAA should help cost-efficient management of photobioreactors in a large scale for the production of value-added products and biofuels from microalgal biomass.

  5. Research on Higher Vocational Student Professional Skill Quality Cultivation Methods%高职学生业务技能素质养成的方法研究

    Institute of Scientific and Technical Information of China (English)

    陈志强

    2015-01-01

    Aiming to the present situation of higher vocational student professional skill quality, it analyzes the research meaning of current higher vocational student features and its professional skill cultivation method. By the curriculum education cyber gamification, skill base training and professional skill manage-ment culture construction, it can work out the problems of higher vocational student profession skill quality cultivation.%针对高职学生的业务技能素质现况,分析目前高职学生的特点及其业务技能素质养成方法的研究意义;通过课程教育网络游戏化、技能基地训练和业务技能管理文化建设等途径,可以较好地解决高职学生业务技能素质养成的感知、熟知和精准问题。

  6. Production Process for Stem Cell Based Therapeutic Implants: Expansion of the Production Cell Line and Cultivation of Encapsulated Cells

    Science.gov (United States)

    Weber, C.; Pohl, S.; Poertner, R.; Pino-Grace, Pablo; Freimark, D.; Wallrapp, C.; Geigle, P.; Czermak, P.

    Cell based therapy promises the treatment of many diseases like diabetes mellitus, Parkinson disease or stroke. Microencapsulation of the cells protects them against host-vs-graft reactions and thus enables the usage of allogenic cell lines for the manufacturing of cell therapeutic implants. The production process of such implants consists mainly of the three steps expansion of the cells, encapsulation of the cells, and cultivation of the encapsulated cells in order to increase their vitality and thus quality. This chapter deals with the development of fixed-bed bioreactor-based cultivation procedures used in the first and third step of production. The bioreactor system for the expansion of the stem cell line (hMSC-TERT) is based on non-porous glass spheres, which support cell growth and harvesting with high yield and vitality. The cultivation process for the spherical cell based implants leads to an increase of vitality and additionally enables the application of a medium-based differentiation protocol.

  7. The Cultivation of Arabidopsis for Experimental Research Using Commercially Available Peat-Based and Peat-Free Growing Media.

    Directory of Open Access Journals (Sweden)

    Tiffany Drake

    Full Text Available Experimental research involving Arabidopsis thaliana often involves the quantification of phenotypic traits during cultivation on compost or other growing media. Many commercially-available growing media contain peat, but peat extraction is not sustainable due to its very slow rate of formation. Moreover, peat extraction reduces peatland biodiversity and releases stored carbon and methane into the atmosphere. Here, we compared the experimental performance of Arabidopsis on peat-based and several types of commercially-available peat-free growing media (variously formed from coir, composted bark, wood-fibre, and domestic compost, to provide guidance for reducing peat use in plant sciences research with Arabidopsis. Arabidopsis biomass accumulation and seed yield were reduced by cultivation on several types of peat-free growing media. Arabidopsis performed extremely poorly on coir alone, presumably because this medium was completely nitrate-free. Some peat-free growing media were more susceptible to fungal contamination. We found that autoclaving of control (peat-based growing media had no effect upon any physiological parameters that we examined, compared with non-autoclaved control growing media, under our experimental conditions. Overall, we conclude that Arabidopsis performs best when cultivated on peat-based growing media because seed yield was almost always reduced when peat-free media were used. This may be because standard laboratory protocols and growth conditions for Arabidopsis are optimized for peat-based media. However, during the vegetative growth phase several phenotypic traits were comparable between plants cultivated on peat-based and some peat-free media, suggesting that under certain circumstances peat-free media can be suitable for phenotypic analysis of Arabidopsis.

  8. Optimization of Escherichia coli cultivation methods for high yield neuropeptide Y receptor type 2 production.

    Science.gov (United States)

    Berger, Christian; Montag, Cindy; Berndt, Sandra; Huster, Daniel

    2011-03-01

    The recombinant expression of human G protein-coupled receptors usually yields low production levels using commonly available cultivation protocols. Here, we describe the development of a high yield production protocol for the human neuropeptide Y receptor type 2 (Y2R), which provides the determination of expression levels in a time, media composition, and process parameter dependent manner. Protein was produced by Escherichia coli in a defined medium composition suitable for isotopic labeling required for investigations by nuclear magnetic resonance spectroscopy. The Y2 receptor was fused to a C-terminal 8x histidine tag by means of the pET vector system for easy one-step purification via affinity chromatography, yielding a purity of 95-99% for every condition tested, which was determined by SDS-PAGE and Western blot analysis. The Y2 receptor was expressed as inclusion body aggregates in complex media and minimal media, using different carbon sources. We investigated the influences of media composition, temperature, pH, and set specific growth rate on cell behavior, biomass wet weight specific and culture volume specific amounts of the target protein, which had been identified by inclusion body preparation, solubilization, followed by purification and spectrometric determination of the protein concentration. The developed process control strategy led to very high reproducibility of cell growth and protein concentrations with a maximum yield of 800 μg purified Y2 receptor per gram wet biomass when glycerol was used as carbon source in the mineral salt medium composition (at 38 °C, pH 7.0, and a set specific growth rate of 0.14 g/(gh)). The maximum biomass specific amount of purified Y2 receptor enabled the production of 35 mg Y2R per liter culture medium at an optical density (600 nm) of 25.

  9. The Effect of Cultivated Wild Ginseng Extract on Preadipocyte Proliferation

    Directory of Open Access Journals (Sweden)

    Byoung-Woo Kim

    2007-12-01

    Full Text Available Objectives : The purpose of this study is to investigate the effects of cultivated wild ginseng extract on primary cultured preadipocyte and adipocytes. Methods : Diminish preadipocyte proliferation does primary role to reduce obesity. So, preadipocytes and adipocytes were performed on cell cultures with using Sprague-Dawley rats and treated with 0.01-1mg/㎖ cultivated wild ginseng extract. Result : At all concentrations, cultivated wild ginseng extract wasn't show the suppress proliferation of preadipocytes significantly and failed to show effects on decomposition of adipocytes except high dosage. Conclusion : Based on these findings, cultivated wild ginseng is not a suitable choice for the treatment of localized obesity.

  10. Cultivation and utilization of specific wood biomass for synthesis of cellulose based bioethanol

    Energy Technology Data Exchange (ETDEWEB)

    Fara, L.; Comaneci, D. [Polytechnic Univ. of Bucharest, Bucharest (Romania). Faculty of Applied Sciences; Cincu, C.; Hubca, G.; Zaharia, C.; Diacon, A. [Polytechnic Univ. of Bucharest, Bucharest (Romania). Faculty of Applied Chemistry; Filat, M.; Chira, D. [Forest Research and Management Inst., Ilfov (Romania); Nutescu, C. [National Wood Inst., Bucharest (Romania); Fara, S. [Inst. for Research and Design of Automation, Bucharest (Romania)

    2010-07-01

    The energetic characteristics of 6 types of poplar clones cultivated for different pedoclimatic conditions in Romania were determined. Four clones were developed in Italy and 2 in Romania. Five experimental cultures were used to analyze the plant survival rate and biomass production rate. After 2 years of study, the Italian clones were found to have very good adaptability to the pedoclimatic conditions in Romania in comparison with local clones. The Italian clones Monviso and AF-6 registered the most substantial growths and the highest resistance to disease. Bioethanol was synthesized by acidic hydrolysis of the cellulose using 2 approaches. In the first approach the lignocellulosic raw material was hydrolyzed with diluted sulfuric acid at 50 degrees C for 24 hours. After filtration, the solid residue was treated with 30 per cent H{sub 2}SO{sub 4} at 100 degrees C for 6 hours. The resulting solutions were neutralized with Ca(OH){sub 2} following another filtration and the resulted solution with pH 6.5 was subjected to fermentation with Saccharomices Cerevisiae. In the second approach the lignocellulosic raw material was subjected to hydrolysis with 10 per cent H{sub 2}SO{sub 4} at 100 degrees C for 4 hours. After filtration, the solid residue was hydrolyzed with 30 per cent H{sub 2}SO{sub 4} at 100 degrees for 6 hours. The solution was neutralized with Ca(OH){sub 2} and subjected to alcoholic fermentation with Saccharomices Cerevisiae. The fermentation took place at 25 degrees C for 72 hours. The results for the two methods were similar.

  11. Model-based design of protected cultivation system - first results and remaining challenges

    NARCIS (Netherlands)

    Henten, van E.J.; Vanthoor, B.H.E.; Stanghellini, C.; Visser, de P.H.B.; Hemming, S.

    2012-01-01

    Abstract: Protected cultivation systems are used throughout the world as a powerful instrument to produce crops. They protect the crops from unfavorable outdoor climate conditions and pests and offer the opportunity to modify the indoor climate to create an environment that is optimal for crop growt

  12. Straighten the Back to Sit: Belly-Cultivation Techniques as "Modern Health Methods" in Japan, 1900-1945.

    Science.gov (United States)

    Wu, Yu-Chuan

    2016-09-01

    In Japan, the first half of the twentieth century saw a remarkable revival of concern with the cultivation of the belly, with a variety of belly-cultivation techniques, particularly breathing exercise and meditative sitting, widely practiced for improving health and treating diseases. This article carefully examines some practitioners' experiences of belly-cultivation practice in attempting to understand its healing effects for them within their life histories and contemporary intellectual, social and cultural contexts. It shows that belly-cultivation practice served as a medium for some practitioners to reflect on and retell their life stories, and that the personal charisma of certain masters and the communities developing around them provided practitioners with a valuable sense of belonging in an increasingly industrialized and urbanized society. Moreover, these belly-cultivation techniques provided an embodied way for some to explore and affirm their sense of self and develop individual identity. While they were increasingly promoted as cultural traditions capable of cultivating national character, they also served as healing practices by inspiring practitioners with a sense of collective identity and purpose. With these analyses, this article sheds light on the complicated meanings of belly-cultivation for practitioners, and provides illustrative examples of the multitude of meanings of the body, bodily cultivation and healing.

  13. Influence of different cultivation methods on carbohydrate and lipid compositions and digestibility of energy of fruits and vegetables

    DEFF Research Database (Denmark)

    Jørgensen, Henry; Knudsen, Knud Erik Bach; Lauridsen, Charlotte

    2012-01-01

    of harvest. Organic foods are defined as products that are produced under controlled cultivation conditions characterized by the absence of synthetic fertilisers and very restricted use of pesticides. Very limited information is available regarding the impact of organic cultivation systems on the composition...

  14. 基于选择试验法的北京市城乡居民耕地保护支付意愿研究%A Study on Willingness of Urban and Rural Residents to Pay for Cultivated Land Preservation Based on Choice Experiment Method

    Institute of Scientific and Technical Information of China (English)

    施园园; 赵华甫; 郧文聚; 汤怀志; 董芳玢

    2016-01-01

    [目的]测算北京市城乡居民耕地保护的支付意愿,检验二者的支付意愿差异,并分析差异形成的原因,为制定面向对象差异化的耕地保护政策提供理论借鉴。[方法]采用选择试验法(CE),R统计及部分经济学模型。[结果]农村居民对保护耕地面积、耕地质量和耕地景观生态环境的支付意愿分别为67.24,113.75,51.47元/a ,而城镇居民的支付意愿分别为58.07,66.40,150.80元/a。[结论]城镇居民的支付意愿明显高于农村居民,个体支付意愿与其文化程度、家庭年总收入、家庭抚养人口数显著相关;从二者耕地功能需求的角度看,城乡居民对耕地各项功能需求最高的分别为生态功能与生产功能,在需求差异的驱动下样本在选择集中做出符合自己需求偏好的选择。%Objective] The aims of this study are to estimate the willingness of residents to pay for farmland protection in Beijing City ,to investigate the differences of willingness between urban and rural residents ,and analyze the driving factors that cause the differences ,in order to provide the theory references for the devel‐opment of object‐oriented differentiated cultivated land protection policy .[Methods] Choice experiment(CE) method was employed .R statistical language software and econometric models was used .[Results] The amount of rural residents willing to pay for the protection of cultivated land area ,quality and landscape ecological environment was 67 .24 ,113 .75 and 51 .47 yuan per year ,respectively ,while the amount of urban residents willing to pay was 58 .07 ,66 .40 and 150 .80 yuan per year ,respectively .[Conclusion] The willing‐ness of urban residents to pay for land protection was significantly higher than that of rural residents ,indi‐vidual’s willingness to pay was positively related to their education ,annual total income of household and dependent population in family

  15. Effect of wastewater-borne bacteria on algal growth and nutrients removal in wastewater-based algae cultivation system.

    Science.gov (United States)

    Ma, Xiaochen; Zhou, Wenguang; Fu, Zongqiang; Cheng, Yanling; Min, Min; Liu, Yuhuan; Zhang, Yunkai; Chen, Paul; Ruan, Roger

    2014-09-01

    Centrate, a type of nutrient-rich municipal wastewater was used to determine the effect of wastewater-borne bacteria on algal growth and nutrients removal efficiency in this study. The characteristics of algal and bacterial growth profiles, wastewater nutrient removal and effect of initial algal inoculums were systematically examined. The results showed that initial algal concentration had apparent effect on bacterial growth, and the presence of bacteria had a significant influence on algal growth pattern, suggesting symbiotic relationship between algae and bacteria at the initial stage of algae cultivation. The maximum algal biomass of 2.01 g/L with 0.1g/L initial algal inoculums concentration can be obtained during algae cultivation in raw centrate medium. The synergistic effect of centrate-borne bacteria and microalgae on algae growth and nutrient removal performance at initial fast growth stage has great potential to be applied to pilot-scale wastewater-based algae wastewater system cultivated in continuous or semi-continuous mode.

  16. Research on the cultivation path of smart home-based care service mode in Internet+ vision

    Directory of Open Access Journals (Sweden)

    Peng Qingchao

    2016-01-01

    Full Text Available Home-based care for the aged is an effective method to solve the problem of caring the aged in China. This thesis analyzes some problems existing in the development of current home-based care service for the aged in our country and the positive effects brought by Internet+ in home-based care service. It proposes a new service mode of care for the aged--Internet+ home-based care service, and explains the establishment of this system and the responsibilities of the participants. Also, it explores the path to realize the establishment of Internet+ home-based care service mode so as to promote the healthy development of home-based care service in China.

  17. GIS-based characterization of the geographic distributions of wild and cultivated populations of the Mesoamerican fruit tree Spondias purpurea (Anacardiaceae).

    Science.gov (United States)

    Miller, Allison J; Knouft, Jason H

    2006-12-01

    Humans are having a profound impact on the geographic distributions of plant populations. In crop species, domestication has been accompanied by the geographic expansion of cultivated populations relative to their wild ancestors. We used a geographical information system (GIS)-based approach to investigate differences in the environmental factors characterizing the geographic distributions of cultivated and wild populations of the Mesoamerican fruit tree Spondias purpurea. Locality data for 86 cultivated and 28 wild S. purpurea populations were used in conjunction with environmental data layers and Maxent, a maximum entropy application for predicting species distributions. Interpredictivity analyses and principal components analysis revealed that the predicted distribution of wild S. purpurea is nested within the cultivated distribution and that the ecological niche (defined by environmental characteristics) of cultivated S. purpurea has expanded relative to that of wild populations. Significant differences between wild and cultivated populations were detected for five environmental variables, corresponding to the expansion of S. purpurea during the domestication process from its native habitat in the Mesoamerican tropical dry forests into less seasonal habitats. These data suggest that humans have altered the range of habitats occupied by cultivated S. purpurea populations relative to their wild progenitors.

  18. Optimizing high strength acetic acid bioprocess by cognitive methods in an unsteady state cultivation.

    Science.gov (United States)

    Arnold, S; Becker, T; Delgado, A; Emde, F; Enenkel, A

    2002-08-07

    Methods of adapting micro-organisms to an inhibiting factor in an active industrial bioprocess were examined with an acetic acid fermentation as model. With the aim of automatic control, a fuzzy-logic system was developed on the basis of the collected knowledge of skilled vinegar brewers. In a first step, this fuzzy system was to assess the actual adaptation degree of the bacteria on the basis of data from robust and reasonably priced sensors. From this information an appropriate setpoint value for the inhibiting factor 'final acid concentration' was derived for each batch cycle. As a result a further acid tolerance was found after several batch cycles. This adaptation effect should be used to increase the product concentration to more than 20 g per 100 ml acetic acid with a high productivity. The stepwise adapted culture was productive over the aimed acetic acid concentration, a 10% improvement of the product formation rate could be found compared with the status before conditioning. High product concentration and increased productivity finally result in shorter cycle times, less transport and storage volumes, an improved utilization of energy and material resources, and, last but not least, they are an essential steps towards the fulfillment of economical and ecological demands.

  19. Optimal conditions of different flocculation methods for harvesting Scenedesmus sp. cultivated in an open-pond system.

    Science.gov (United States)

    Chen, Lu; Wang, Cunwen; Wang, Weiguo; Wei, Jiang

    2013-04-01

    The effects of culture medium pH, flocculant type (FeCl3, Al2(SO4)3, Alum, Ca(OH)2, chitosan, polyacrylamide), dosage and sedimental time on flocculation efficiency of harvesting Scenedesmus sp. cultivated in an open-pond system were investigated. Meanwhile, the relation between initial biomass concentration and the flocculant dosage needed was also investigated. The results from this work indicated that the flocculation efficiency achieved 97.4% after 10 min of sedimentation when the pH was adjusted to be 11.5, without adding flocculants. FeCl3 and chitosan showed a good flocculation efficiency at dosage of 0.15 and 0.08 g/L, respectively without pH adjustment. The flocculation efficiency increased from 49.74% to 90.63% when the final medium pH was adjusted to 6 after adding 0.1 g/L Alum. An increment from 68.18% to 92.84% was observed after adding 0.1 g/L Al2(SO4)3 followed by pH adjustment. Finally, the most suitable flocculation method was discussed in this paper.

  20. Methods in Logic Based Control

    DEFF Research Database (Denmark)

    Christensen, Georg Kronborg

    1999-01-01

    Desing and theory of Logic Based Control systems.Boolean Algebra, Karnaugh Map, Quine McClusky's algorithm. Sequential control design. Logic Based Control Method, Cascade Control Method. Implementation techniques: relay, pneumatic, TTL/CMOS,PAL and PLC- and Soft_PLC implementation. PLC-design met......Desing and theory of Logic Based Control systems.Boolean Algebra, Karnaugh Map, Quine McClusky's algorithm. Sequential control design. Logic Based Control Method, Cascade Control Method. Implementation techniques: relay, pneumatic, TTL/CMOS,PAL and PLC- and Soft_PLC implementation. PLC...

  1. Entropy-based benchmarking methods

    OpenAIRE

    2012-01-01

    We argue that benchmarking sign-volatile series should be based on the principle of movement and sign preservation, which states that a bench-marked series should reproduce the movement and signs in the original series. We show that the widely used variants of Denton (1971) method and the growth preservation method of Causey and Trager (1981) may violate this principle, while its requirements are explicitly taken into account in the pro-posed entropy-based benchmarking methods. Our illustrati...

  2. Activity based costing (ABC Method

    Directory of Open Access Journals (Sweden)

    Prof. Ph.D. Saveta Tudorache

    2008-05-01

    Full Text Available In the present paper the need and advantages are presented of using the Activity BasedCosting method, need arising from the need of solving the information pertinence issue. This issue has occurreddue to the limitation of classic methods in this field, limitation also reflected by the disadvantages ofsuch classic methods in establishing complete costs.

  3. Optical coherence tomography-based topography determination of corneal grafts in eye bank cultivation

    Science.gov (United States)

    Damian, Angela; Seitz, Berthold; Langenbucher, Achim; Eppig, Timo

    2017-01-01

    Vision loss due to corneal injuries or diseases can be treated by transplantation of human corneal grafts (keratoplasty). However, quality assurance in retrieving and cultivating the tissue transplants is confined to visual and microbiological testing. To identify previous refractive surgery or morphological alterations, an automatic, noncontact, sterile screening procedure is required. Twenty-three corneal grafts have been measured in organ culture with a clinical spectral-domain optical coherence tomographer. Employing a biconic surface fit with 10 degrees of freedom, the radii of curvature and conic constants could be estimated for the anterior and posterior corneal surfaces. Thereupon, central corneal thickness, refractive values, and astigmatism have been calculated. Clinical investigations are required to elaborate specific donor-host matching in the future.

  4. Estimation of the stand ages of tropical secondary forests after shifting cultivation based on the combination of WorldView-2 and time-series Landsat images

    Science.gov (United States)

    Fujiki, Shogoro; Okada, Kei-ichi; Nishio, Shogo; Kitayama, Kanehiro

    2016-09-01

    We developed a new method to estimate stand ages of secondary vegetation in the Bornean montane zone, where local people conduct traditional shifting cultivation and protected areas are surrounded by patches of recovering secondary vegetation of various ages. Identifying stand ages at the landscape level is critical to improve conservation policies. We combined a high-resolution satellite image (WorldView-2) with time-series Landsat images. We extracted stand ages (the time elapsed since the most recent slash and burn) from a change-detection analysis with Landsat time-series images and superimposed the derived stand ages on the segments classified by object-based image analysis using WorldView-2. We regarded stand ages as a response variable, and object-based metrics as independent variables, to develop regression models that explain stand ages. Subsequently, we classified the vegetation of the target area into six age units and one rubber plantation unit (1-3 yr, 3-5 yr, 5-7 yr, 7-30 yr, 30-50 yr, >50 yr and 'rubber plantation') using regression models and linear discriminant analyses. Validation demonstrated an accuracy of 84.3%. Our approach is particularly effective in classifying highly dynamic pioneer vegetation younger than 7 years into 2-yr intervals, suggesting that rapid changes in vegetation canopies can be detected with high accuracy. The combination of a spectral time-series analysis and object-based metrics based on high-resolution imagery enabled the classification of dynamic vegetation under intensive shifting cultivation and yielded an informative land cover map based on stand ages.

  5. Development of a facile droplet-based single-cell isolation platform for cultivation and genomic analysis in microorganisms

    Science.gov (United States)

    Zhang, Qiang; Wang, Tingting; Zhou, Qian; Zhang, Peng; Gong, Yanhai; Gou, Honglei; Xu, Jian; Ma, Bo

    2017-01-01

    Wider application of single-cell analysis has been limited by the lack of an easy-to-use and low-cost strategy for single-cell isolation that can be directly coupled to single-cell sequencing and single-cell cultivation, especially for small-size microbes. Herein, a facile droplet microfluidic platform was developed to dispense individual microbial cells into conventional standard containers for downstream analysis. Functional parts for cell encapsulation, droplet inspection and sorting, as well as a chip-to-tube capillary interface were integrated on one single chip with simple architecture, and control of the droplet sorting was achieved by a low-cost solenoid microvalve. Using microalgal and yeast cells as models, single-cell isolation success rate of over 90% and single-cell cultivation success rate of 80% were demonstrated. We further showed that the individual cells isolated can be used in high-quality DNA and RNA analyses at both gene-specific and whole-genome levels (i.e. real-time quantitative PCR and genome sequencing). The simplicity and reliability of the method should improve accessibility of single-cell analysis and facilitate its wider application in microbiology researches. PMID:28112223

  6. Pilot scale land-based cultivation of Saccharina latissima Linnaeus at southern European climate conditions: Growth and nutrient uptake at high temperatures

    DEFF Research Database (Denmark)

    Azevedo, Isabel C.; Silva Marinho, Goncalo; Silva, Diogo M.

    2016-01-01

    of integrated multi-trophic aquaculture (IMTA) systems, presenting good results considering both growth and biofiltration performance. In the present work, the cultivation of S. latissima in a pilot land-based system was performed in order to assess the efficiency of two different methods: tumbling in the water...... during high temperature periods. Densities around 8 kg m− 3 were effective in keeping epiphytes development low. This system may be used for seaweed monoculture or as a biofilter component of IMTA systems.......Saccharina latissima is a cold water seaweed species with commercial potential. The northern Portuguese coast is the southern distribution limit of the species, where some dispersed populations can be found. S. latissima has been identified as being a potential candidate for monoculture or as part...

  7. 基于脱钩指数的2020年江苏省耕地保有量目标分析%Target Analysis of Cultivated Land Protection Area in Jiangsu Province in 2020 Based on Decoupling Index

    Institute of Scientific and Technical Information of China (English)

    肖丽群; 吴群

    2012-01-01

    脱钩理论是近年来国际上在测度经济增长与物质消耗之间关系过程中提出的一种理论方法。本研究引入脱钩理论中的脱钩指数及其评价标准,采用历史分析与未来预测相结合的方法,估算了2020年江苏省耕地保有量目标实现的难易程度及其耕地保有量缺口。并且利用试算内插法,测算出了实现耕地保有量目标时的脱钩指数值。研究结果表明:①近30年来,江苏省经济增长与耕地消耗的脱钩指数一直呈现波动下降的趋势,主要是结构效应和政策效应共同作用的结果;②实现2020年耕地保有量目标压力巨大,脱钩指数只有维持在不大于0.0012的水平,才有可能实现耕地保有量目标。因此,在保证经济增长的同时实现耕地保有量规划目标,则必须转变经济增长方式,采取更多积极的政策措施。%Rapid growth of economy inevitably requires a large quantity of land resources. In the next 10 years, China’s economy will enjoy rapid development, so limited cultivated land resources will be faced with great pressure. At the same time, economic growth will also be the main cause of cultivated land consumption. Based on the domestic and foreign researches, this paper has analyzed the relationship between economic growth and cultivated land consumption and estimated the difficulty of achieving the cultivated land protection target. Decoupling theory is a theoretical approach put forward in the process of measuring the relationship between economic growth and substance consumption by international researchers in recent years. The word"decoupling"is derived from physics. Agricultural trade negotiators, economists and policymakers use "decoupling"to interpret the departure relationship between agricultural trade and policy measures. This paper has estimated the difficulty of achieving the target of cultivated land protection area and its gap in Jiangsu Province in 2020 by introducing

  8. Influence of cultivated landscape composition on variety resistance: an assessment based on wheat leaf rust epidemics.

    Science.gov (United States)

    Papaïx, Julien; Goyeau, Henriette; Du Cheyron, Philippe; Monod, Hervé; Lannou, Christian

    2011-09-01

    In plant pathology, the idea of designing variety management strategies at the scale of cultivated landscapes is gaining more and more attention. This requires the identification of effects that take place at large scales on host and pathogen populations. Here, we show how the landscape varietal composition influences the resistance level (as measured in the field) of the most grown wheat varieties by altering the structure of the pathogen populations. For this purpose, we jointly analysed three large datasets describing the wheat leaf rust pathosystem (Puccinia triticina/Triticum aestivum) at the country scale of France with a Bayesian hierarchical model. We showed that among all compatible pathotypes, some were preferentially associated with a variety, that the pathotype frequencies on a variety were affected by the landscape varietal composition, and that the observed resistance level of a variety was linked to the frequency of the most aggressive pathotypes among all compatible pathotypes. This data exploration establishes a link between the observed resistance level of a variety and landscape composition at the national scale. It illustrates that the quantitative aspects of the host-pathogen relationship have to be considered in addition to the major resistance/virulence factors in landscape epidemiology approaches.

  9. Characteristics of a hydrated, alginate-based delivery system for cultivation of the button mushroom.

    Science.gov (United States)

    Romaine, C P; Schlagnhaufer, B

    1992-09-01

    The production of the button mushroom Agaricus bisporus with mycelium-colonized alginate pellets as an inoculant of the growing medium was investigated. Pellets having an irregular surface and porous internal structure were prepared by complexing a mixture of 1% sodium alginate, 2 to 6% vermiculite, 2% hygramer, and various concentrations of Nutrisoy (soy protein) with calcium chloride. The porous structure allowed the pellets to be formed septically and then inoculated and colonized with the fungus following sterilization. By using an enzyme-linked immunosorbent assay (ELISA) to estimate fungal biomass, the matrix components of the pellet were found to be of no nutritive value to A. bisporus. Pellets amended with Nutrisoy at a concentration of 0.5 to 8% supported extensive mycelial growth, as determined by significantly increased ELISA values, with a concentration of 4% being optimal and higher concentrations proving inhibitory. The addition of hydrated, mycelium-invaded pellets to the compost or casing layer supported the thorough colonization of the growing substrate and culminated in the formation of mushrooms that showed normal development and typical morphology. Yields and sizes of mushrooms were comparable from composts seeded with either colonized pellets or cereal grain spawn. Similarly, amending the casing layer with pelletized-mycelium-colonized compost resulted in a 2- to 3-day-earlier and more-synchronous emergence of mushrooms than with untreated casing. This technology shows the greatest potential as a pathogen-free inoculant of the casing layer in the commercial cultivation of mushrooms.

  10. Evaluation on Fuzzy Variable Weight of Cultivated Land Fertility Based on GIS

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    [Objective] The modified variable weights based on constant weight and in- troduced theory of equalization function would better incorporate authentic index weights and make evaluation results of fertility more scientific. [Method] In Gaozhou City, the final weights of influential factors can be determined with the help of GIS and as per AHP and theory of variable weights. In addition, farmland fertility was e- valuated in an automatic and quantitative way and the spatial distribution pattern was analyzed as per fuzzy comprehensive evaluation. [Result] For farmlands at 58 505.027 8 hm2 in the city, farmlands from grade 1 to grade 8 account for 3.62%, 18.27%, 33.15%, 26.96%, 13.66%, 3.29%, 0.81% and 0.24%, respectively, which is in consistent with local condition. [Conclusion] These results have been applied di- rectly in test regions and constitute a rewarding exploration for fertility evaluation in South China.

  11. Fungal cultivation on glass-beads

    DEFF Research Database (Denmark)

    Droce, Aida; Sørensen, Jens Laurids; Giese, Henriette;

    Transcription of various bioactive compounds and enzymes are dependent on fungal cultivation method. In this study we cultivate Fusarium graminearum and Fusarium solani on glass-beads with liquid media in petri dishes as an easy and inexpensive cultivation method, that resembles in secondary meta...... metabolite production to agar-cultivation but with an easier and more pure RNA-extraction of total fungal mycelia....

  12. 基于胜任力的高校高层次人才培养的探析%Exploration on High-level Personnel Cultivating Based on Competency in University

    Institute of Scientific and Technical Information of China (English)

    王懿; 刘卫东

    2012-01-01

    本文将胜任力理论与高校高层次人才培养实践相结合,阐述了胜任力理论的基本内涵,分析了高校高层次人才培养存在的问题以及基于胜任力的高层次人才培养的意义,探讨了高层次人才胜任力研究的步骤和方法,为高校高层次人才培养改革提供一个新的思路.%On the basis of competency theory and high-level personnel cultivating of university, the study stated simply connotation of competency, analysed problem of high-level personnel cultivating of university, and importance of high-level personnel cultivating based on competency in university, and explored process and method about analysis of high-level personnel competency, in order to offer a reference for reform of high-level personnel cultivating.

  13. Cultivating engineering ethics and critical thinking: a systematic and cross-cultural education approach using problem-based learning

    Science.gov (United States)

    Chang, Pei-Fen; Wang, Dau-Chung

    2011-08-01

    In May 2008, the worst earthquake in more than three decades struck southwest China, killing more than 80,000 people. The complexity of this earthquake makes it an ideal case study to clarify the intertwined issues of ethics in engineering and to help cultivate critical thinking skills. This paper first explores the need to encourage engineering ethics within a cross-cultural context. Next, it presents a systematic model for designing an engineering ethics curriculum based on moral development theory and ethic dilemma analysis. Quantitative and qualitative data from students' oral and written work were collected and analysed to determine directions for improvement. The paper also presents results of an assessment of this interdisciplinary engineering ethics course. This investigation of a disaster is limited strictly to engineering ethics education; it is not intended to assign blame, but rather to spark debate about ethical issues.

  14. Comparative Analysis and Cultivation of New Agricultural Business Entities Based on Comparison of Family Farms and Professional Cooperatives

    Institute of Scientific and Technical Information of China (English)

    Fang; YANG; Xingxi; LIU; Yingliang; ZHANG

    2014-01-01

    Cultivating new bussiness entities is the key to speeding up improving new agricultural production and operating system.This study discussed two representative entities under the agricultural production and operating system based on household contract management,namely,family farms and cooperatives.It introduced current development status of these two entities in Chongqing region,and discussed prominent problems of understanding,management,fund,personnel,technology,information,and system in the development process.In line with common problems such as single production structure and short supply of agricultural insurance,and different problems like organizational degree,it made comparative analysis.Finally,it is concluded that different institutional arrangement and pertinent support policy according to different development characteristics.

  15. Gas chromatography/mass spectrometry-based metabolic profiling and differentiation of ginseng roots according to cultivation age using variable selection.

    Science.gov (United States)

    Park, Hae Eun; Lee, Seok-Young; Hyun, Sun-Hee; Kim, Da Yeon; Marriott, Philip J; Choi, Hyung-Kyoon

    2013-01-01

    Ginseng roots are an important herbal resource worldwide, and the adulteration of ginseng with age is recognized as a serious problem. It is therefore crucial to develop objective criteria or standard protocols for differentiating ginseng root samples according to their cultivation age. The reported study used GC/MS combined with multivariate statistical analysis with variable selection to obtain metabolic profiling and an optimal partial least squares-discriminant analysis (PLS-DA) model for the differentiation of ginseng according to cultivation age. Relative levels of various metabolites, such as amino acids, alcohols, fatty acids, organic acids, and sugars, were measured for various ginseng cultivation ages. Increasing cultivation age resulted in the production of higher levels of panaxynol and panaxydol, which are active polyacetylene compounds in ginseng. In addition, optimized PLS-DA models for the prediction of ginseng age were obtained by selecting variables based on a variable importance in the projection cut-off value of 1.3. Proline, glucaric acid, mannose, gluconic acid, glucuronic acid, myoinositol, panaxydol, and panaxynol are suggested as key and relevant compounds with which to differentiate the age of ginseng samples. The findings of this study suggest that GC/MS-based metabolic profiling can be used to differentiate ginseng samples according to cultivation age.

  16. Single-base resolution maps of cultivated and wild rice methylomes and regulatory roles of DNA methylation in plant gene expression

    Directory of Open Access Journals (Sweden)

    Li Xin

    2012-07-01

    Full Text Available Abstract Background DNA methylation plays important biological roles in plants and animals. To examine the rice genomic methylation landscape and assess its functional significance, we generated single-base resolution DNA methylome maps for Asian cultivated rice Oryza sativa ssp. japonica, indica and their wild relatives, Oryza rufipogon and Oryza nivara. Results The overall methylation level of rice genomes is four times higher than that of Arabidopsis. Consistent with the results reported for Arabidopsis, methylation in promoters represses gene expression while gene-body methylation generally appears to be positively associated with gene expression. Interestingly, we discovered that methylation in gene transcriptional termination regions (TTRs can significantly repress gene expression, and the effect is even stronger than that of promoter methylation. Through integrated analysis of genomic, DNA methylomic and transcriptomic differences between cultivated and wild rice, we found that primary DNA sequence divergence is the major determinant of methylational differences at the whole genome level, but DNA methylational difference alone can only account for limited gene expression variation between the cultivated and wild rice. Furthermore, we identified a number of genes with significant difference in methylation level between the wild and cultivated rice. Conclusions The single-base resolution methylomes of rice obtained in this study have not only broadened our understanding of the mechanism and function of DNA methylation in plant genomes, but also provided valuable data for future studies of rice epigenetics and the epigenetic differentiation between wild and cultivated rice.

  17. Cultivating New-type Farmers Based on the Theory of Human Resources Development

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Under the direction of theory of human resources development,this thesis analyzes the impact of rural human resources development oncultivating new-type farmers.Firstly,it increases the input of rural basic education;secondly,it reinforces the vocational education and technologytraining;thirdly,it promotes the rural medical and public health services;fourthly,it quickens the rural labor transfer.The status quo of China’s ruralhuman resources has been analyzed as follows:in terms of the quantity of rural human resources,the status quo is large and quick-developed baseof rural human resources,high labor participatory rate,and constitution of low age;in terms of the quality of rural human resources,the status quois the ubiquitous low quality of rural human resources,low technological level of rural human resources,and overall low physical quality of farmers;in terms of the structure of rural human resources,the status quo is the irrational industrial structure distribution and imbalanced regional structuredistribution.The thesis also discusses the edification of theory of human resources development in cultivating new-type farmers.First,in terms ofthe control over quantity of rural human resources,it is to keep the stability of family planning policy,and expedite the transfer of rural surplus la-bor;second,in terms of promoting the quality of rural human resources,it is to bolster the development of reserve rural labor force resources,toconstruct the adult educational training system with Chinese characteristics,and to build rural primary health care system;third,in terms of adjus-ting the structure of rural human resources,it is to perfect rural human resources market,and adjust rural economical structure and talents struc-ture.

  18. Entropy-based benchmarking methods

    NARCIS (Netherlands)

    Temurshoev, Umed

    2012-01-01

    We argue that benchmarking sign-volatile series should be based on the principle of movement and sign preservation, which states that a bench-marked series should reproduce the movement and signs in the original series. We show that the widely used variants of Denton (1971) method and the growth pre

  19. Population-based resequencing analysis of wild and cultivated barley revealed weak domestication signal of selection and bottleneck in the Rrs2 scald resistance gene region.

    Science.gov (United States)

    Fu, Yong-Bi

    2012-02-01

    Many plant disease resistance (R) genes have been cloned, but the potential of utilizing these plant R-gene genomic resources for genetic inferences of plant domestication history remains unexplored. A population-based resequencing analysis of the genomic region near the Rrs2 scald resistance gene was made in 51 accessions of wild and cultivated barley from 41 countries. Fifteen primer pairs were designed to sample the genomic region with a total length of 10 406 bp. More nucleotide diversity was found in wild (π = 0.01846) than cultivated (π = 0.01507) barley samples. Three distinct groups of 29 haplotypes were detected for all 51 samples, and they were well mixed with wild and cultivated barley samples from different countries and regions. The neutrality tests by Tajima's D were not significant, but a significant (P domestication. Together, the domestication signal in the genomic region was weak both in human selection and domestication bottleneck.

  20. A Spiral Step-by-Step Educational Method for Cultivating Competent Embedded System Engineers to Meet Industry Demands

    Science.gov (United States)

    Jing,Lei; Cheng, Zixue; Wang, Junbo; Zhou, Yinghui

    2011-01-01

    Embedded system technologies are undergoing dramatic change. Competent embedded system engineers are becoming a scarce resource in the industry. Given this, universities should revise their specialist education to meet industry demands. In this paper, a spirally tight-coupled step-by-step educational method, based on an analysis of industry…

  1. Key Technology for Cultivation of Virus-free Potato Original Species with Aeroponics Method%气雾栽培脱毒马铃薯关键技术

    Institute of Scientific and Technical Information of China (English)

    南雄雄; 王昊; 时新宁; 李永华

    2012-01-01

    Cultivation of micro-potato original species with aeroponics method is a relatively new and efficient method of breeding original species of potatoes,it is also easy to regulate and control.It was conducted to explore the key technology of cultivation of micro-potato original species with aeroponics method.The study was carried out in the aspects of major equipment installation selection,selection and planting of seedlings,key technologies of seedling management,nutrient management in the growing season,potato harvesting and storage and other key technologies,which was to develop a more appropriate key technologies integration of potato cultivation with aeroponics method and provide theoretical and practical guidance.%马铃薯无基质气雾栽培技术是国内比较新型的快速繁育微型薯的一种方法,它同时具有高效和条件易控的优点。研究从马铃薯气雾栽培主要设备安装与选择,苗木的选择与定植,苗期管理关键技术、生长期营养液管理及马铃薯采收及储存等关键技术环节进行研究总结,制定出了较为适宜的马铃薯气雾栽培关键技术体系,为实际生产提供理论和实践指导。

  2. Study on Compensation Standard of Land Expropriation Based on Comprehensive Value of Cultivated Land%基于耕地综合价值的土地征收补偿标准研究

    Institute of Scientific and Technical Information of China (English)

    诸培新; 卜婷婷; 吴正廷

    2011-01-01

    The existing compensation of cultivated land expropriation only covers cultivated land value of agricultural use and employment, and neglects other values such as ecological value, which leads to a series of social and economic problems related to cultivated land expropriation. Therefore, it is necessary to protect farmers' land rights and cultivated land resources to establish a reasonable standard of cultivated land expropriation. Based on the theory of comprehensive value of cultivated land resources, land expropriation compensation needs to cover three kinds of value; agricultural economic value, social security value and ecological value. Then, different methods are employed to calculate various values of land. The return flow discount method is used for agricultural economic value measurement, and cost substitution method for social value calculation, and of contingent value method for ecological value. Last, Nanjing City of Jiangsu Province is taken as an example to establish expropriation compensation standard adapted to comprehensive land value in 2007. The result of the case study shows that compensation received by farmers according to comprehensive compensation standard shall be 6.6 times of the existing compensation standard for land expropriation. Because the new land compensation can internalize external value of cultivated land such as social stability value and ecological values, and effectively eliminate the market failure during the cultivated land expropriation, it will improve the efficiency of cultivated land conversion to non-agricultural use. In addition, new standard of land compensation is much lower than the current benchmark price of construction land; therefore, it is feasible in economy and acceptable in society.%由于现行耕地征收补偿仅对耕地的农业经济价值以及部分就业保障价值进行补偿,忽视了耕地的其他价值,导致了耕地征收中的一系列社会经济问题产生.因此,合理制定

  3. Design and Implementation of Emergy-Based Sustainability Decision Assessment System for Protected Grape Cultivation

    OpenAIRE

    2015-01-01

    The characteristic “high input and output” in protected production has caused some environmental and ecological issues. Hence, emergy-based sustainability assessments are necessary and valuable. However, traditional emergy analysis is time consuming, tedious, and inefficient. Such disadvantages can be addressed by the integration of emergy analysis with information technology. This paper reports the development of the emergy-based sustainability decision assessment system (ESDAS) for protecte...

  4. Cultivating Professional Allies for Sexual Minority Youth: A Community-Based Educational Intervention.

    Science.gov (United States)

    Craig, Shelley L; Doiron, Christopher; Dillon, Frank

    2015-01-01

    Sexual minority youth (SMY) face multiple risks in their daily lives that may influence their need for supportive care. Health and social service providers have unique opportunities to provide culturally competent services to these youth. This article describes a community-based educational intervention created to increase providers' knowledge, skills, and intention to support SMY. Based on the Information-Motivation-Behavioral Skills model, this pilot study found that for a diverse sample of multidisciplinary professionals (n = 2,850), the odds of behavioral intention (BI) to support SMY were significantly higher when trainings were relevant to the professionals' experience (OR = 1.3), were sensitive (OR = 1.3), developed skills (OR = 1.1), and incorporated policy (OR = 1.2). Implications for the delivery of community-based trainings are provided.

  5. Optimization of Milk-Based Medium for Efficient Cultivation of Bifidobacterium pseudocatenulatum G4 Using Face-Centered Central Composite-Response Surface Methodology

    Directory of Open Access Journals (Sweden)

    Khalilah Abdul Khalil

    2014-01-01

    Full Text Available This study was undertaken to optimize skim milk and yeast extract concentration as a cultivation medium for optimal Bifidobacteria pseudocatenulatum G4 (G4 biomass and β-galactosidase production as well as lactose and free amino nitrogen (FAN balance after cultivation period. Optimization process in this study involved four steps: screening for significant factors using 23 full factorial design, steepest ascent, optimization using FCCD-RSM, and verification. From screening steps, skim milk and yeast extract showed significant influence on the biomass production and, based on the steepest ascent step, middle points of skim milk (6% wt/vol and yeast extract (1.89% wt/vol were obtained. A polynomial regression model in FCCD-RSM revealed that both factors were found significant and the strongest influence was given by skim milk concentration. Optimum concentrations of skim milk and yeast extract for maximum biomass G4 and β-galactosidase production meanwhile low in lactose and FAN balance after cultivation period were 5.89% (wt/vol and 2.31% (wt/vol, respectively. The validation experiments showed that the predicted and experimental values are not significantly different, indicating that the FCCD-RSM model developed is sufficient to describe the cultivation process of G4 using skim-milk-based medium with the addition of yeast extract.

  6. Fruit protected cultivation in China

    Institute of Scientific and Technical Information of China (English)

    Gao Huajun; Wang Saoming; Wang Jiaxi

    2003-01-01

    Protected fruit cultivation in China has developed very quickly from the early 1990s, and now it is animportant branch in fruit cultivation. A brief review including fruit species, developing history, growing area, output, anddistribution in the whole country is made in the paper. Characteristics of the dominant kinds of greenhouse,environmental control methods, and standards of temperature, humidity, light and CO2 for different fruit species arepresented. Information on varieties, growing benefits, special management practices and other aspects of the main fruitspecies used for protected cultivation are also presented.

  7. 浅谈案例教学法与培养创新人才%On the Case Method and Cultivating Innovative Talents

    Institute of Scientific and Technical Information of China (English)

    马金友; 吴时国

    2012-01-01

    培养创新人才是素质教育和创新教育的要求.文章简要介绍了案例教学具有培养创新人才的特征,通过比较传统教学法与案例教学法,彰显了案例教学法对培养创新人才的优势,进一步论证了案例教学对培养学生创新能力的价值,即案例教学有利于培养学生创新思维能力,有利于刺激学生求知创新的欲望,有利于激活学生创新天性.有利于培养学生创新意识.并提出案例教学重在精选案例:案例要有针对性、现实性、典型性、基本性和有多样性.%The cultivation of innovative talents is the requirement of the quality education and innovation education. The article briefly describes that the case teaching has the characteristics of nurturing creative talents. Through comparing the traditional leaching methods and case teaching, the advantages of the case teaching method to cultivate innovative talents are highlighted, and its value is further demonstrated, that is, cases teaching can cultivate students' creative thinking ability, stimulate the students' desire of innovation, activate students' innovative nature and develop students' awareness of innovation. The paper proposes that the case teaching focuses on the selection of cases and cases should be targeted, realistic and typical, basic and diversity.

  8. Cultivating strategic thinking skills.

    Science.gov (United States)

    Shirey, Maria R

    2012-06-01

    This department highlights change management strategies that may be successful in strategically planning and executing organizational change initiatives. With the goal of presenting practical approaches helpful to nurse leaders advancing organizational change, content includes evidence-based projects, tools, and resources that mobilize and sustain organizational change initiatives. In this article, the author presents an overview of strategic leadership and offers approaches for cultivating strategic thinking skills.

  9. Metagenomic and Cultivation-Based Analysis of Novel Microorganisms and Functions in Metal-Contaminated Environments

    OpenAIRE

    Yelton, Alexis Pepper

    2012-01-01

    Some bacteria and archaea have evolved metabolic strategies that enable them to live in environments contaminated by toxic metals. In fact, many bacteria and archaea take advantage of the redox sensitivity of these very same metals to gain energy via anaerobic respiration. Here, metagenomic techniques were developed and applied along with conventional physiological and ecological methods to elucidate multiple modes of adaptation of bacteria and archaea in metal-contaminated acid mine drainage...

  10. Quantification of polyphenols and pharmacological analysis of water and ethanol-based extracts of cultivated agarwood leaves.

    Science.gov (United States)

    Ito, Tetsuro; Kakino, Mamoru; Tazawa, Shigemi; Watarai, Tatsuya; Oyama, Masayoshi; Maruyama, Hiroe; Araki, Yoko; Hara, Hideaki; Iinuma, Munekazu

    2012-01-01

    Mangiferin (3) and genkwanin 5-O-β-primeveroside (5) are the two major bioactive polyphenols with laxative property present in the extracts of agarwood (Aquilaria sinensis) leaves (AL). Here we developed an HPLC method to determine these bioactive components and four other major polyphenols in AL extracts and evaluated the pharmacological equivalence of organic and water extracts. Using mobile phase gradient conditions combined with UV detection at 330 nm, all six compounds were separated and we determined the relative extraction ratios of the six compounds present in A. sinensis extracts that were prepared under different conditions and compared the contents of the two laxative polyphenols present in the 60% ethanol extracts of A. sinensis and A. crassna. The polyphenols present in water extracts of 13 commercially cultivated A. crassna plants have also been analyzed. The laxative properties of 60% ethanol and four water extracts of A. crassna were evaluated by the frequency and weight of stools in loperamide-induced constipation model mice. The pharmacological equivalence of 60% ethanol extract and hot water (95°C) extract was identified in mice.

  11. Alternative method of inoculum and spawn production for the cultivation of the edible brazilian mushroom Pleurotus ostreatoroseus SING.

    Science.gov (United States)

    Rosado, Fábio Rogério; Kemmelmeier, Carlos; Da Costa, Sandra Maria Gomes

    2002-01-01

    Efficiency of solid and liquid inocula and their use for spawn production were compared so that improved cultivation conditions for the edible mushroom Pleurotus ostreatoroseus could be tested. Solid and liquid inocula were prepared respectively with Potato Dextrose Agar (PDA) and Liquid Potato Dextrose (LPD). Wheat grains and cotton residues were used as substrates for spawn preparation. Inoculum types did not affect the development of P. ostreatoroseus, and LPD spawns were cheaper, more homogenous, less contaminated. Decomposition activity of mushroom growth, as a percentage of organic matter loss (OML), was higher in the wheat grain spawn and was not influenced by the inoculum type. Advantages in the use of cotton residue for spawn production were longer storage time, lower contamination and reduced costs. The cotton residue substrate may be also used for the production of mushroom fruiting bodies.

  12. Characterisation of hexane-degrading microorganisms in a biofilter by stable isotope-based fatty acid analysis, FISH and cultivation.

    Science.gov (United States)

    Friedrich, Michèle M; Lipski, André

    2010-01-01

    The hexane-degrading bacterial community of a biofilter was characterised by a combination of stable isotope-based phospholipid fatty acid analyses, fluorescence in situ hybridisation and cultivation. About 70 bacterial strains were isolated from a full-scale biofilter used for treatment of hexane containing waste gas of an oil mill. The isolation approach led to 16 bacterial groups, which were identified as members of the Alpha-, Beta- and Gammaproteobacteria, Actinobacteria and Firmicutes. Three groups showed good growth on hexane as the sole source of carbon. These groups were allocated to the genera Gordonia and Sphingomonas and to the Nevskia-branch of the Gammaproteobacteria. Actively degrading populations in the filter material were characterised by incubation of filter material samples with deuterated hexane and subsequent phospholipid fatty acid analysis. Significant labelling of the fatty acids 16:1 cis10, 18:1 cis9 and 18:0 10methyl affiliated the hexane-degrading activity of the biofilter with the isolates of the genus Gordonia. In vitro growth on hexane and in situ labelling of characteristic fatty acids confirmed the central role of these organisms in the hexane degradation within the full-scale biofilter.

  13. Characterizing diversity based on nutritional and bioactive compositions of yam germplasm (Dioscorea spp. commonly cultivated in China

    Directory of Open Access Journals (Sweden)

    Zhi-Gang Wu

    2016-04-01

    Full Text Available Yams (Dioscorea spp. are widely cultivated as edible resources and medical materials in China. Characterizing chemical compositions in yam germplasm is crucial to determine their diversity and suitability for food and medicine applications. In this study, a core germplasm containing 25 yam landraces was used to create an effective classification of usage by characterizing their nutritive and medicinal compositions. All studied landraces exhibited high contents of starch from 60.7% to 80.6% dry weight (DW, protein (6.3–12.2% DW, minerals (especially Mg 326.8–544.7 mg/kg DW, and essential amino acids. Allantoin and dioscin varied considerably, with values of 0.62–1.49% DW and 0.032–0.092% DW, respectively. The quality variability of 25 yam landraces was clearly separated in light of UPGMA clustering and principal component analysis (PCA. Using an eigenvalue ≥1 as the cutoff, the first three principal components accounted for most of the total variability (62.33%. Classification was achieved based on the results of the measured parameters and principal component analysis scores. The results are of great help in determining appropriate application strategies for yam germplasm in China.

  14. Recommendations for the regionalizing of coffee cultivation in Colombia: a methodological proposal based on agro-climatic indices.

    Science.gov (United States)

    García L, Juan Carlos; Posada-Suárez, Húver; Läderach, Peter

    2014-01-01

    The Colombian National Federation of Coffee Growers (FNC) conducted an agro-ecological zoning study based on climate, soil, and terrain of the Colombian coffee-growing regions (CCGR) located in the tropics, between 1° and 11.5° N, in areas of complex topography. To support this study, a climate baseline was constructed at a spatial resolution of 5 km. Twenty-one bioclimatic indicators were drawn from this baseline data and from yield data for different coffee genotypes evaluated under conditions at eight experimental stations (ESs) belonging to the National Center for Coffee Research (CENICAFÉ). Three topographic indicators were obtained from a digital elevation model (DEM). Zoning at a national level resulted in the differentiation of 12 agro-climatic zones. Altitude notably influenced zone differentiation, however other factors such as large air currents, low-pressure atmospheric systems, valleys of the great rivers, and physiography also played an important role. The strategy of zoning according to coffee-growing conditions will enable areas with the greatest potential for the development of coffee cultivation to be identified, criteria for future research to be generated, and the level of technology implementation to be assessed.

  15. Bacterial diversity assessed by cultivation-based techniques shows predominance of Staphylococccus species on coins collected in Lisbon and Casablanca.

    Science.gov (United States)

    de Carvalho, Carla C C R; Caramujo, Maria José

    2014-04-01

    Money is usually considered a source of infections, yet survival of bacteria on dry metal surfaces is limited. The aim of this work was to assess the bacterial numbers and diversity on coins collected in Casablanca and Lisbon as these two cities, on different continents and only 585 km apart, have diverse cultural habitats, but have similar climate. A cultivation-based characterisation of the bacterial community showed that a relatively low number of cells per area of coin were found on both Moroccan Dirhams and Euros (0.014 and 0.125 colony-forming units (CFU) mm(-2) , respectively). Most of the bacterial isolates were located near the rim of the coins, and coins collected in Europe contained more CFU/area of coin. Coins collected in Europe and transported in pockets presented a higher number of bacterial isolates (maximum 0.125 CFU mm(-2) ) than coins transported in wallets (maximum 0.042 CFU mm(-2) ), regardless of the gender of the person transporting them, suggesting that temperature and moisture might be key parameters for bacterial survival on metallic coins. Bacterial diversity was higher for coins collected in Casablanca relative to coins collected in Lisbon, and there was no evidence that it was dependent on the gender of the person transporting the coins or the place of transport (pockets or wallets). Curiously, the percentage of Staphylococcus strains was 44% of the total isolates on both currencies.

  16. Predictive mapping of soil organic carbon in wet cultivated lands using classification-tree based models

    DEFF Research Database (Denmark)

    Kheir, Rania Bou; Greve, Mogens Humlekrog; Bøcher, Peder Klith

    2010-01-01

    field measurements in the area of interest (Denmark). A large number of tree-based classification models (588) were developed using (i) all of the parameters, (ii) all Digital Elevation Model (DEM) parameters only, (iii) the primary DEM parameters only, (iv), the remote sensing (RS) indices only, (v...... the geographic distribution of SOC across Denmark using remote sensing (RS), geographic information systems (GISs) and decision-tree modeling (un-pruned and pruned classification trees). Seventeen parameters, i.e. parent material, soil type, landscape type, elevation, slope gradient, slope aspect, mean curvature......) selected pairs of parameters, (vi) soil type, parent material and landscape type only, and (vii) the parameters having a high impact on SOC distribution in built pruned trees. The best constructed classification tree models (in the number of three) with the lowest misclassification error (ME...

  17. Evaluation of disinfectant efficacy against hepatitis C virus using a RT-PCR-based method.

    Science.gov (United States)

    Charrel, R N; de Chesse, R; Decaudin, A; De Micco, P; de Lamballerie, X

    2001-10-01

    The methods traditionally used to evaluate the antiviral activity of antiseptics and disinfectants are based on cell cultures. However, such methods are not applicable to non-cultivable viruses such as hepatitis C (HCV). Therefore, in this case, virucidal activity is normally tested using surrogate viruses able to grow in cell culture. This paper describes a RT-PCR method for testing antiseptic/disinfectant activity against HCV, as a model for non-cultivable viruses. A chlorine-based agent used for skin and tissues, and a 2% glutaraldehyde solution used for endoscope disinfection, were the test materials. The results are discussed in the light of the use of these agents. The method is simple, fast and inexpensive, and could be used for tests on other viruses with minor modification.

  18. Feasibility Study on Soilless Cultivation of Organic Ginseng

    Institute of Scientific and Technical Information of China (English)

    Yuji JIN; Hongyan ZHAO; Xiangguo LI; Renzhe PIAO

    2015-01-01

    Based on the present situation and problems concerning ginseng cultivation as well as soilless cultivation features,we analyze the growth indicators and input-output ratio of different ginseng cultivation patterns,and conform that the soilless cultivation technology for organic ginseng is feasible. And this technology provides theoretical basis and technological feasibility for the sustainable development of ginseng industry.

  19. DISPLACEMENT BASED SEISMIC DESIGN METHODS.

    Energy Technology Data Exchange (ETDEWEB)

    HOFMAYER,C.MILLER,C.WANG,Y.COSTELLO,J.

    2003-07-15

    A research effort was undertaken to determine the need for any changes to USNRC's seismic regulatory practice to reflect the move, in the earthquake engineering community, toward using expected displacement rather than force (or stress) as the basis for assessing design adequacy. The research explored the extent to which displacement based seismic design methods, such as given in FEMA 273, could be useful for reviewing nuclear power stations. Two structures common to nuclear power plants were chosen to compare the results of the analysis models used. The first structure is a four-story frame structure with shear walls providing the primary lateral load system, referred herein as the shear wall model. The second structure is the turbine building of the Diablo Canyon nuclear power plant. The models were analyzed using both displacement based (pushover) analysis and nonlinear dynamic analysis. In addition, for the shear wall model an elastic analysis with ductility factors applied was also performed. The objectives of the work were to compare the results between the analyses, and to develop insights regarding the work that would be needed before the displacement based analysis methodology could be considered applicable to facilities licensed by the NRC. A summary of the research results, which were published in NUREGICR-6719 in July 2001, is presented in this paper.

  20. Yield-enhancing and tuber-downsizing effects of transplantation cultivation method of case-held tuber seedlings in the sweet potato cultivar Beniharuka

    Directory of Open Access Journals (Sweden)

    Katsuki Adachi

    2016-01-01

    Full Text Available We developed transplantation cultivation method of case-held tuber seedlings (CTS, which was derived from direct planting method of seed tubers, and applied this method to the sweet potato cultivar Beniharuka. A plastic case made of polypropylene was designed for cultivation of CTS. Seed tubers of cultivar Beniharuka in the range of 30–80 g were cut in half. The half-cut tubers were placed inside the plastic cases, and the cases were filled with a commercial soil mix. The case-held tubers were incubated under natural sunlight in a glass house. After 3–4 wk, the CTS were transplanted into a field. Mother tuber (seed tuber enlargement was suppressed by the plastic confinement of the cases, and daughter tubers were formed above the case as vine-root-originated tubers. In the field experiments in 2012 and 2013, daughter tuber yields were increased 19% and 21% by case-held tuber seedling transplanting (CTST over conventional vine-planting (VP, the number of daughter tubers per plant in CTST were 36 and 68% higher than in VP, and the mother tuber yields were limited to 2.1 and 4.3% of the total fresh yield of mother and daughter tubers, respectively in 2012 and 2013. Application of CTST method to cultivar Beniharuka enhanced tuber yield, increased the number of daughter tubers per plant, downsized daughter tubers compared to VP, and mother tuber enlargement was suppressed by case-holding. The CTST method is expected to produce more and smaller good in shape tubers of cultivar Beniharuka compared to VP.

  1. CULTIVATION OF CLADOCERAN (CLADOCERA FOR INCREASING PROVISION OF YOUNG-OF-THE-YEAR CARP (CYPRINUS CARPIO WITH NATURAL FEEDS (REVIEW

    Directory of Open Access Journals (Sweden)

    A. Tuchapska

    2014-06-01

    Full Text Available Purpose. Natural feeds are important in pond fish diet because they contain all nutrients necessary for fish growth and development. The share of natural feeds in fish diet has great effect on fish growth and immunity, assimilation of artificial feeds. The main way of assured procurement of natural feeds for fish feeding at different stages of their development is artificial cultivation of aquatic organisms. However, cultivation of natural feeds is not virtually performed in aquaculture enterprises of Ukraine, therefore an analysis of available data on zooplankton cultivation is important for looking for optimal and economically profitable methods of enrichment of pond fish diet with natural feeds. Methodology. Methods of cladoceran cultivation were the object of the study, material for the study – literature data on ways and methods of zooplankton cultivation. Findings. Cultivation of various species of zooplankton is performed for feeding of pond fish on different life stages. Main object of cultivation in aquaculture is Daphnia magna Straus, juvenile forms of which are consumed by fish larvae, while adult organisms are the most valuable for yearlings and older fish. The efficiency of hydrobiont cultivation highly depends on the selected object, containers, where cladocerans are cultivated, optimum conditions, peculiarities of water supply, species, and application of fertilizers and feeds. Originality. The highest production of zooplankton can be obtained when cultivating D. magna in tanks with continuous flow and in net cages installed in ponds under condition of ensuring requirements of the culture in bacterial and algae feeds (due to application of fertilizers and feeding with feeds and microalgae taking into account their presence in water, which is in the tank-cultivator. Practical value. Simplicity of the methods and high efficiency of zooplankton cultivation for preparation of pond fish juveniles is the basis of its wide use in

  2. Cellulase and xylanase productions by isolated Amazon Bacillus strains using soybean industrial residue based solid-state cultivation

    Directory of Open Access Journals (Sweden)

    Heck Júlio X.

    2002-01-01

    Full Text Available In Brazil, a large amount of a fibrous residue is generated as result of soybean (Glycine max protein production. This material, which is rich in hemicellulose and cellulose, can be used in solid state cultivations for the production of valuable metabolites and enzymes. In this work, we studied the bioconversion of this residue by bacteria strains isolated from water and soil collected in the Amazon region. Five strains among 87 isolated bacteria selected for their ability to produce either celullases or xylanases were cultivated on the aforementioned residue. From strain BL62, identified as Bacillus subtilis, it was obtained a preparation showing the highest specific cellulase activity, 1.08 UI/mg protein within 24 hours of growth. Concerning xylanase, the isolate BL53, also identified as Bacillus subtilis, showed the highest specific activity for this enzyme, 5.19 UI/mg protein within 72 hours of cultivation. It has also been observed the production of proteases that were associated with the loss of cellulase and xylanase activities. These results indicated that the selected microorganisms, and the cultivation process, have great biotechnological potential.

  3. Dynamic of China's cultivated land and landcover changes of its typical regions based on remote sensing data

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Using the multi-temporal Landsat data and survey data of national resources, the authors studied the dynamics of cultivated land and landcover changes of typical ecological regions in China. The results of investigation showed that the whole distribution of the cultivated land shifted to Northeast and Northwest China, and as a result, the ecological quality of cultivated land dropped down. The seacoast and cultivated land in the area of Yellow River Mouth expanded by an increasing rate of 0.73 km?a-1, with a depositing rate of 2.1 km?a-1. The desertification area of the dynamic of Horqin Sandy Land increased from 60.02% of the total land area in1970s to 64.82% in1980s but decreased to 54.90% in early 1990s. As to the change of North Tibet lakes, the water area of the Namu Lake decreased by 38.58 km2 from year 1970 to 1988, with a decreasing rate of 2.14 km2?a-1.

  4. Cultivating Engineering Ethics and Critical Thinking: A Systematic and Cross-Cultural Education Approach Using Problem-Based Learning

    Science.gov (United States)

    Chang, Pei-Fen; Wang, Dau-Chung

    2011-01-01

    In May 2008, the worst earthquake in more than three decades struck southwest China, killing more than 80,000 people. The complexity of this earthquake makes it an ideal case study to clarify the intertwined issues of ethics in engineering and to help cultivate critical thinking skills. This paper first explores the need to encourage engineering…

  5. Dendrobium officinale cliff epiphytic cultivation method%铁皮石斛悬崖附生栽培技术研究

    Institute of Scientific and Technical Information of China (English)

    斯金平; 陈梓云; 刘京晶; 朱玉球; 蔡国见; 黄柄荣; 章焜一; 金传高

    2015-01-01

    以铁皮石斛设施栽培投入大、药材功效不如野生等问题为导向,通过水分调控、崖壁坡度选择,研究悬崖附生铁皮石斛生长情况、农艺性状、产量、多糖及醇溶性浸出物含量.结果表明,将铁皮石斛附生于85 ~ 90°悬崖表面,在生长季节喷水1 ~2 h·d-1的条件下,即使在裸露的环境中,不用基质,不施肥料也能够较好的生长,获得较高的产量,其形态与悬崖上野生铁皮石斛无异;多糖与醇溶性浸出物含量显著高于设施基质栽培,并与萌蘖生理年龄密切相关,逆境有利于铁皮石斛多糖与醇溶性浸出物等功效成分的积累.%To solve the issues of costly planting of facility cultivation method and inferior efficacy than wild herbs of Dendrobium officinale,the cliff epiphytic cultivation method was studied.To research the growth,agronomic traits,yield,polysaccharide and alcohol-soluble extract contents were measured on the D.officinale from different water regulation and cliff slope gradients treatments.The results showed that D.officinale epiphytic at 85°-90° cliff and sprayed water 1-2 h · d-1 at the growing season can get better growth and obtain high yield,and the morphology has no different from wild cliff D.officinale,even in the environments without shade.The contents of polysaccharide and alcohol-soluble extract are closely related to the physiological ages,but significantly higher than the facility cultivation.It is possible that environmental stresses benefit the accumulation of polysaccharides,alcohol-soluble extract and other efficient ingredients.

  6. Comparative Study of Rice Morphogenesis with Different Cultivation Methods%不同栽培方式下水稻形态发生比较研究

    Institute of Scientific and Technical Information of China (English)

    严定春

    2012-01-01

    The aim of this study was to compare the morphological characteristics of rice ( Oryza sativa L. ) with different cultivation methods and investigate the dynamics of organ growth and development characteristics of different rice cultivars. [ Method ] Based on continuous field observation and destructive sampling over a growing season, detailed organ morphological data were obtained including leaf length, node number, plant height, tiller number, leaf angle, leaf area and specific leaf weight, to compare organ morphological differences a-mong 4 rice cultivars of Baidao (indica) , Jinnanfeng (japonica) , 9325 (japonica) and 9915 (japonica) with 3 cultivation methods of field planting in Weigang, pot planting in Weigang, and field planting in Jiangpu. [Result] Maximum leaf length of each node gradually increased at the early growth stage and decreased at the later growth stage, the relationship between maximum leaf length and node can be described with the equations y -ax and y = ax + b; node number, growth duration, leaf length and plant height of pot planting rice in Weigang were smaller than that of the other two field planting methods; the relationship between plant height and sunshine duration, plant height and GDD (growing degree days) can be described with the equation y = ax + b, 19.23 ℃ o d of GDD ( ≥ 10℃ o d) and 8.12 h of sunshine duration were required to increase 1 cm of plant height; plant height, tiller number, and leaf area of Baidao were higher than that of the other 3 japonica rice cultivars, but the specific leaf weight and leaf angle were smaller. .[ Conclusion ] Comparison of morphological characteristic differences among rice cultivars is an important way to select water-saving and drought-tolerant rice varieties. In this study, the experimental results can be integrated into a rice functional-architectural model to simulate rice organ growth dynamics in a three-dimensional space, thereby providing reference for selection of water

  7. Cultivating nutrition

    OpenAIRE

    Levin, Carol E; Long, Jennifer; Simler, Kenneth R.; Johnson-Welch, Charlotte

    2003-01-01

    "Over the past decade, donor-funded policies and programs designed to address undernutrition in the Global South have shifted away from agriculture-based strategies toward nutrient supplementation and food fortification programs. Given the potential benefits resulting from agriculture-based nutrition interventions, this study uses Q methodology to explore the views of a range of stakeholders from both developed and developing countries on the value of—and constraints related to—gender-sensiti...

  8. Bioreactors for high cell density and continuous multi-stage cultivations: options for process intensification in cell culture-based viral vaccine production.

    Science.gov (United States)

    Tapia, Felipe; Vázquez-Ramírez, Daniel; Genzel, Yvonne; Reichl, Udo

    2016-03-01

    With an increasing demand for efficacious, safe, and affordable vaccines for human and animal use, process intensification in cell culture-based viral vaccine production demands advanced process strategies to overcome the limitations of conventional batch cultivations. However, the use of fed-batch, perfusion, or continuous modes to drive processes at high cell density (HCD) and overextended operating times has so far been little explored in large-scale viral vaccine manufacturing. Also, possible reductions in cell-specific virus yields for HCD cultivations have been reported frequently. Taking into account that vaccine production is one of the most heavily regulated industries in the pharmaceutical sector with tough margins to meet, it is understandable that process intensification is being considered by both academia and industry as a next step toward more efficient viral vaccine production processes only recently. Compared to conventional batch processes, fed-batch and perfusion strategies could result in ten to a hundred times higher product yields. Both cultivation strategies can be implemented to achieve cell concentrations exceeding 10(7) cells/mL or even 10(8) cells/mL, while keeping low levels of metabolites that potentially inhibit cell growth and virus replication. The trend towards HCD processes is supported by development of GMP-compliant cultivation platforms, i.e., acoustic settlers, hollow fiber bioreactors, and hollow fiber-based perfusion systems including tangential flow filtration (TFF) or alternating tangential flow (ATF) technologies. In this review, these process modes are discussed in detail and compared with conventional batch processes based on productivity indicators such as space-time yield, cell concentration, and product titers. In addition, options for the production of viral vaccines in continuous multi-stage bioreactors such as two- and three-stage systems are addressed. While such systems have shown similar virus titers compared to

  9. In vitro cultivation of rat bone marrow mesenchymal stem cells and establishment of pEGFP/Ang-1 transfection method

    Directory of Open Access Journals (Sweden)

    Xiu-Qun Zhang

    2014-09-01

    Conclusions: Adherence screening method and density gradient centrifugation can be effective methods to obtain BMSCs with high purity and rapid proliferation. Besides, the expression of transfected recombinant plasmid pEGFP/Ang-1 in rat BMSCs is satisfactory.

  10. Multi-Suitability Evaluation of Cultivated Land in Houhu Farm Area Based on GIS and Niche-Fitness

    Institute of Scientific and Technical Information of China (English)

    LIU Li-wei; NIE Yan; ZHOU Yong

    2005-01-01

    The niche-fitness and limited factors of rice, wheat, cotton and rape in Houhu Farm area of Jianghan Plain are analyzed by weighted average model and limited factor model. The niche-fitness theory and GIS are applied to the research on multi-suitability evaluation. The high values of the niche-fitness (the maximum is 0.958) indicate that the environmental conditions basically meet the requirements of crops in the area. Suitability evaluation of single crop shows that the degrees of suitability are satisfactory. Comprehensive suitability evaluation shows that the acreage for grade 1 (highly suitable for cultivation) accounts for 18.16% of the total cultivated land area, whereas the acreage for grade 3 (critically suitable) and grade 4 (unsuitable) only for 8.9%. The main factors limiting the potential land productivity are cation exchange capacity (CEC), available phosphorus content and accessibility level of road.

  11. Plant growth and cultivation.

    Science.gov (United States)

    Podar, Dorina

    2013-01-01

    There is a variety of methods used for growing plants indoor for laboratory research. In most cases plant research requires germination and growth of plants. Often, people have adapted plant cultivation protocols to the conditions and materials at hand in their own laboratory and growth facilities. Here I will provide a guide for growing some of the most frequently used plant species for research, i.e., Arabidopsis thaliana, barley (Hordeum vulgare) and rice (Oryza sativa). However, the methods presented can be used for other plant species as well, especially if they are related to the above-mentioned species. The presented methods include growing plants in soil, hydroponics, and in vitro on plates. This guide is intended as a starting point for those who are just beginning to work on any of the above-mentioned plant species. Methods presented are to be taken as suggestive and modification can be made according to the conditions existing in the host laboratory.

  12. Research on Monitoring Area Division of Quality Grade Changes in County Cultivated Land and Technology of Deploying Monitoring Point

    Institute of Scientific and Technical Information of China (English)

    Wei; WEI; Lijun; LIAO; Jianxin; YU

    2013-01-01

    It is an important means in management of improving both the quality and quantity of cultivated land to monitor grade changes in cultivated land quality. How to deploy monitoring network system and its point reasonably and roundly are the key to the technology of monitoring grade changes in cultivated land quality by monitoring grade changes in cultivated land quality dynamically in order to obtain the information to the index of cultivated land quality and its changes based on the existing achievements of farmland classification and grading. Spatial analysis method is used to demarcate monitoring area and deploy monitoring point according to ARCGIS,of which the result can meet the demand for monitoring grade changes in cultivated land.

  13. Impacts of energy crop cultivation on nature and landscape. Development and application of an evaluation method; Auswirkungen des Energiepflanzenanbaus auf Natur und Landschaft. Entwicklung und Anwendung einer Bewertungsmethode

    Energy Technology Data Exchange (ETDEWEB)

    Wiehe, Julia

    2011-08-15

    For long-term planning, knowledge about the interrelationship of effects of the cultivation method and the sensitivity of ecological balance is essential. Hence, the objective of this thesis is the development of a method for the evaluation of the impacts of bioenergy crop production for biogas use on the natural environment. The developed method is in alignment with existing methods. It is also in alignment with those methods used within the practice of landscape planning, so that the method as well as the derived conclusions can be implemented into landscape planning practice in the future. The evaluation method has been applied in the three model regions Hildesheim, Soltau-Fallingbostel and Emsland. These test areas represent different physical regions in Lower Saxony and typical agricultural production conditions. On the basis of these results, general statements on the impact of bioenergy crop production on the ecological balance of the area can be made. [German] Grundlage fuer eine vorausschauende Steuerung des Ausbaus erneuerbarer Energien ist die Kenntnis der Zusammenhaenge der Wirkung des Energiepflanzenanbaus und der Empfindlichkeit des Naturhaushaltes. Ziel der Arbeit ist daher die Erarbeitung einer Methode zur umfassenden Bewertung dieser Auswirkungen auf den Naturhaushalt. Die Methode orientiert sich an bereits bestehenden und in der Praxis der Landschaftsplanung angewendeten Bewertungsmethoden, so dass sie ebenso wie die daraus abgeleiteten Schlussfolgerungen zukuenftig Eingang in die Planungspraxis finden kann. Die Bewertungsmethode wird in den drei Modellregionen Hildesheim, Soltau-Fallingbostel und Emsland angewendet, mit denen die verschiedenen Naturraeume und fuer Niedersachsen typischen landwirtschaftlichen Produktionsbedingungen abgebildet werden. Auf Grundlage dieser Ergebnisse koennen dann allgemeine Aussagen zu den Auswirkungen des Energiepflanzenanbaus auf den Naturhaushalt gemacht werden.

  14. Effects of Cultivation Methods on Plant Growth and Yield of Summer-planting Peanut%栽培方式对夏直播花生植株生长及产量的影响

    Institute of Scientific and Technical Information of China (English)

    杨富军; 赵长星; 闫萌萌; 王月福; 王铭伦

    2013-01-01

    为了探讨麦后夏直播花生的适宜栽培方式及其作用效果,为指导生产实践提供依据.在田间试验条件下,以‘青花7号’花生品种为材料,研究了不同栽培方式对夏直播花生植株生长和产量的影响.结果表明:采用花生夏直播高产保护性栽培法,可矮化株高,主茎高度较地膜覆盖的减少15.7%,防止徒长和倒伏;促进营养器官干物质积累,增加有效开花数、有效果针数;促进荚果发育,单株结果数较其他处理增加0.8~2.0个,公斤果数减少23.8~66.5个;显著增加干物质向荚果中分配的比例,经济系数提高0.08~0.17,荚果产量提高5.5%~29.2%.地膜覆盖的植株生育状况和产量优于露地栽培的,秸秆还田的优于不还田的.研究认为,在本试验条件下,采用花生夏直播高产保护性栽培法是取得夏直播花生高产的最佳栽培方式.%The paper explored the suitable cultivation methods and their effects of summer-planting peanut after winter wheat harvest, in order to provide a basis for guiding the production practices of peanut. Under field conditions, ' Qinghua 7' was used to study the influences of cultivation methods on plant growth and yield of summer-planting peanut. The results showed that: compared with conventional cultivation methods, high-yield protective cultivation style of summer-planting peanut could dwarf the plant height, so that the main height under the method decreased 15.7% compared with plastic film mulching methods, which could effectively prevent excessive growth and lodging of peanut. High-yield protective cultivation style of summer-planting peanut promoted dry matter accumulation of vegetative organs of peanut increased the number of effective flowering and fruit pin of peanut. Moreover, high-yield protective cultivation style of summer-planting peanut could promote the pod development of peanut, and the pod number per plant increased from 0.8 to 2.0 than that under

  15. PBL教学法在实验教学中对学生批判性思维能力的培养%Application of PBL teaching method in cultivation of critical thinking ability of students in experimental teaching

    Institute of Scientific and Technical Information of China (English)

    秦海宏; 葛伟; 孙雪洁

    2013-01-01

    The PBL teaching method is a teaching mode based on problems.It has significant effect on the formation of crilical thinking ability of the nursery students in experimental teaching.In our study,the PBL was adopted in the experimental group,while traditional teaching method was used in the control group.CCTDI-CV was applied to assess the critical thinking ability of the students in these above two groups.It was found that the critical thinking ability of the students in the experimental group is significantly higher than that in the control group.This suggested that the PBL is beneficial to cultivate the critical thinking ability of the students.This paper provides an evidence for improving the teaching mode and way of the cultivation of critical thinking ability in nursery practice teaching.%PBL教学是以问题为基础的模式,在实验教学中对培养学生批判性思维能力的效果显著.在对实验组采用PBL教学法教学、对照组采用传统的实验教学方法教学的基础上,应用CCTDI-CV评价2种教学方法对学生批判性思维能力的影响.经分析发现,PBL教学组学生的批判性思维能力高于对照组,两组间差异显著.实验教学中的PBL教学模式有助于学生批判性思维能力的培养.

  16. In vitro cultivation of rat bone marrow mesenchymal stem cells and establishment of pEGFP/Ang-1 transfection method

    Institute of Scientific and Technical Information of China (English)

    Xiu-Qun Zhang; Long Wang; Shu-Li Zhao; Wei Xu

    2014-01-01

    Objective:To obtain the bone marrow mesenchymal stem cells (BMSCs), complete phenotypic identification and successfully transfect rat BMSCs by recombinant plasmid pEGFP/Ang-1. Methods:BMSCs were isolated from bone marrow using density gradient centrifugation method and adherence screening method, and purified. Then the recombinant plasmid pEGFP/Ang-1 was used to transfect BMSCs and the positive clones were obtained by the screen of G418 and observed under light microscopy inversely. Green fluorescent exhibited by protein was enhanced to measure the change time of the expression amount of Ang-1. Results: BMSCs cell lines were obtained successfully by adherence screening method and density gradient centrifugation. Ang-1 recombinant plasmid was transfected smoothly into rat BMSCs, which can express Ang-1 for 3 d and decreased after 7 d. Conclusions:Adherence screening method and density gradient centrifugation can be effective methods to obtain BMSCs with high purity and rapid proliferation. Besides, the expression of transfected recombinant plasmid pEGFP/Ang-1 in rat BMSCs is satisfactory.

  17. COMPANY VALUATION METHODS BASED ON PATRIMONY

    Directory of Open Access Journals (Sweden)

    SUCIU GHEORGHE

    2013-02-01

    Full Text Available The methods used for the company valuation can be divided into 3 main groups: methods based on patrimony,methods based on financial performance, methods based both on patrimony and on performance. The companyvaluation methods based on patrimony are implemented taking into account the balance sheet or the financialstatement. The financial statement refers to that type of balance in which the assets are arranged according to liquidity,and the liabilities according to their financial maturity date. The patrimonial methods are based on the principle thatthe value of the company equals that of the patrimony it owns. From a legal point of view, the patrimony refers to allthe rights and obligations of a company. The valuation of companies based on their financial performance can be donein 3 ways: the return value, the yield value, the present value of the cash flows. The mixed methods depend both onpatrimony and on financial performance or can make use of other methods.

  18. 高职人才培养机理研究:产业集群创新系统的视角%Research on Talent Cultivation Mechanism of the Higher Vocational Education Based on Industry Cluster Groups

    Institute of Scientific and Technical Information of China (English)

    毛才盛; 吕慈仙

    2012-01-01

    本文通过梳理产业集群创新系统相关理论,结合高职人才培养模式特征,基于产业集群与人才培养的耦合机理结构,提出了产业集群与人才培养的耦合度模型,分析产业集群下高职人才培养模式存在着专业设置与市场需求脱节、人才培养资源共享机制不健全、产业与人才培养互动机制缺失等问题,并提出实现产业集群与人才培养的良性耦合机制的对策。%With analysing industry cluster groups theory and talent cultivation features of high vocational education,coupling mechanism between industry cluster groups and talent cultivation,this paper proposes the coupling model of industry cluster groups and talent cultivation,analyses the problems existing in higher vocational talent cultivation based on the industry cluster groups such as no tight coupling between professional setting and market demand,no strong talent cultivation resource sharing mechanism,no information sharing mechanism between industry and talent cultivation,and puts forward the suggestions on how to realize the coupling mechanism between industry cluster and talent cultivation.

  19. Exploratory research on course teaching of architectural design based on ability cultivation of practice and innovation - Take course teaching of high-rise building design as an example

    Directory of Open Access Journals (Sweden)

    Gao Ming

    2016-01-01

    Full Text Available Under current disciplinary development reform and the objective of strengthening ability cultivation of practice innovation, this thesis makes comprehensive analysis of the problems existing in “high-rise building design” teaching for undergraduates majoring in architecture; and proposes new philosophies of focusing on teaching process, opening up joint school management and research-oriented teaching in combination of construction transformation demands. Based on the above background, this thesis also proposes the teaching conception of establishing multi-platform frame. Moreover, it discusses corresponding platform establishment and teaching design in combination of educational reform practice.

  20. Objective, Way and Method of Faculty Management Based on Ergonomics

    Science.gov (United States)

    WANG, Hong-bin; Liu, Yu-hua

    2008-01-01

    The core problem that influences educational quality of talents in colleges and universities is the faculty management. Without advanced faculty, it is difficult to cultivate excellent talents. With regard to some problems in present faculty construction of colleges and universities, this paper puts forward the new objectives, ways and methods of…

  1. An interactive segmentation method based on superpixel

    DEFF Research Database (Denmark)

    Yang, Shu; Zhu, Yaping; Wu, Xiaoyu

    2015-01-01

    This paper proposes an interactive image-segmentation method which is based on superpixel. To achieve fast segmentation, the method is used to establish a Graphcut model using superpixels as nodes, and a new energy function is proposed. Experimental results demonstrate that the authors' method has...... excellent performance in terms of segmentation accuracy and computation efficiency compared with other segmentation algorithm based on pixels....

  2. Digital Autofocusing Method Based on Contourlet Transform

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The autofocusing technique based on contourlet transform is discussed in this paper and an autofocusing method is proposed for images with much information in certain directions. The experimental results show that theproposed method can focus accurately and the sensitivity ratio is higher than that of the other autofocusing methods based on conventional image processing

  3. 县域耕地后备资源与规划期内数量质量并重的占补平衡分析%Analysis on county based reserved resource for cultivated land and quality-quantity requisition-compensation balance in planning period

    Institute of Scientific and Technical Information of China (English)

    高星; 吴克宁; 郧文聚; 唐程杰; 许实; 麻战洪

    2015-01-01

    耕地后备资源是耕地的重要补充来源,在耕地占补平衡中具有重要作用,但在补充耕地能力上长期存在偏主观、重数量等情况。利用国土部新一轮耕地后备资源调查评价试点县成果,在新增耕地系数和等级折算系数的基础上提出了耕地数量与耕地数量质量并重的平衡测算方法,分析县域规划期内实现耕地占补平衡的能力。以湖南省沅江市为例,测算至2020年实现数量平衡和数量质量平衡需开发的耕地后备资源面积。结果显示,满足耕地数量平衡需开发耕地后备资源面积为1083.37 hm2;满足数量质量并重平衡,最低需开发耕地后备资源面积1083.37 hm2,最高需开发1351.65 hm2。通过测算,沅江市耕地后备资源开发可满足县域耕地占补数量、质量平衡的需要,但存在较大压力,尤以数量质量并重平衡为甚。因此,明晰县域耕地后备资源补充耕地的能力有利于合理安排新增建设项目,确保实现土地利用总体规划保护耕地的目标。%Reserved resource for cultivated land is an important supplement to cultivated land, and it plays an important role in requisition-compensation balance, however, it has disadvantages of subjectiveness and emphasizing on quantity in cultivated land supplement. It is necessary to play the role of farmland reserve resources to replenish cultivated land for the realization of the goal of farmland protection in the overall planning period of county land use. Data used for study are from the results of the new round of ‘survey and evaluation of reserved resource for cultivated land’. A balancing method, which focused on both quantity and quality and was based on the ratio of added cultivated land and grade conversion coefficient, were proposed to analyze the capability of requisition-compensation balance in the planning period. And we analyzed the capacity to achieve the balance of arable land in the

  4. GIS-based assessment of land suitability for alfalfa cultivation: a case study in the dry continental steppes of northern China

    Directory of Open Access Journals (Sweden)

    Fei Deng

    2014-03-01

    Full Text Available Alfalfa (Medicago sativa L. is the most valuable perennial forage grass in northern China. We selected 12 ecological criteria and 4 socioeconomic criteria to calculate the suitability of land for alfalfa cultivation in the Xilingol League of the Inner Mongolia Autonomous Region. We combined ecological suitability assessment with fuzzy analysis to standardize the criteria. We used the analytical hierarchy process to determine the weight of these criteria, and used multi-criterion decision analysis (MCDA to aggregate the criteria. We then calculated the suitability score for each evaluation unit. The suitability was divided into highly, moderately, and marginally suitable, or unsuitable, using the geoprocessing module of ArcGIS 9.3 according to the FAO land suitability classification. We found that highly suitable areas covered 10,799.84 km2, accounting for 5.3% of the total area, and unsuitable areas covered 99,235.87 km2, accounting for 48.9% of the total area. Combining the fuzzy analysis method with the theory of ecological suitability and the MCDA method to evaluate the suitability of land for alfalfa cultivation provided insights that can guide decisionmakers and farmers to make more practical and scientific decisions.

  5. 模糊聚类模型在昌平区耕地质量评价中的应用%Cultivated land gradation study of Changping District in Beijing based on fuzzy clustering model

    Institute of Scientific and Technical Information of China (English)

    望勇; 陈宇晖

    2015-01-01

    耕地质量评价是新一轮国土资源大调查的项目之一,准确掌握县域内耕地质量状况十分必要。以北京市昌平区为研究区,将模糊理论、自组织特征映射( self-organizing feature maps,SOFM)与GIS相结合,构造出SOFM-GIS聚类模型,并开发出评价工具,对研究区耕地进行自然等级划分。通过在昌平地区的实验表明,利用SOFM-GIS聚类模型评价耕地质量结果有效,说明SOFM-GIS聚类模型能很好地对耕地质量等级进行划分,并借助GIS的可视化功能显示耕地质量的分异特征,为相似地区耕地质量评价提供参考。%Cultivated land quality assessment is one of the projects in the new round of land resources surveying, and it is necessary to clarify the quality of cultivated land in Changping District of Beijing. In view of the shortage of cultivated land graduation, the authors combined the fuzzy theory and SOFM with GIS to establish the cultivated land graduation model, and used the model to graduate cultivated land of Changping District in Beijing. The research results show that the method has the advantages of obtaining stable and reliable results. The SOFM-GIS clustering model can be used to evaluate the quality of cultivated land and, with the visualization capabilities of GIS, to display distribution characteristics of cultivated land. In addition, it also provides a reference method for evaluating the cultivated land quality in similar areas.

  6. Research on the Teaching Reform of Exhibition Market-ing Based on the Cultivation of Professional Ability%基于职业能力培养的"会展营销"课程教学改革研究

    Institute of Scientific and Technical Information of China (English)

    张杰丽

    2016-01-01

    "会展营销"课程在学生职业能力培养方面的目标包含市场调查能力、商务沟通能力、营销策划能力、销售管理能力和客户服务能力;在此基础上提出了"会展营销"课程的教学改革应从课程内容的优化、教学方法的创新、实践教学内涵的提升、考核评价体系的创新等四个维度展开的建议.%In the cultivation of students' professional abilities, ex-hibition marketing aims at cultivating students' market research ability, business communication ability, marketing planning abil-ity, sales management ability and customer service ability. Based on the above objectives, this paper proposes that the teaching re-form of exhibition marketing should be implemented from course content optimization, teaching method innovation, practice teach-ing improvement and evaluation system innovation.

  7. High-density Linkage Map of Cultivated Allotetraploid Cotton Based on SSR, TRAP, SRAP and AFLP Markers

    Institute of Scientific and Technical Information of China (English)

    Jiwen Yu; Shuxun Yu; Cairui Lu; Wu Wang; Shuli Fan; Meizhen Song; Zhongxu Lin; Xianlong Zhang; Jinfa Zhang

    2007-01-01

    A high-density linkage map was constructed for an F2 population derived from an interspecific cross of cultivated allotetraploid species between Gossyplum hirsutum L. and G. barbadense L. A total of 186 F2 individuals from the interspecific cross of "CRI 36 × Hai 7124" were genotyped at 1 252 polymorphic loci including a novel marker system,target region amplification polymorphism (TRAP). The map consists of 1 097 markers, including 697 simple sequence repeats (SSRs), 171 TRAPs, 129 sequence-related amplified polymorphisms, 98 amplified fragment length polymorphisms, and two morphological markers, and spanned 4 536.7 cM with an average genetic distance of 4.1 cM per marker. Using 45 duplicated SSR loci among chromosomes, 11 of the 13 pairs of homologous chromosomes were identified in tetraplold cotton. This map will provide an essential resource for high resolution mapping of quantitative trait loci and molecular breeding in cotton.

  8. Predictive analysis on the amount of cultivated land transfer based on different targets%基于不同目标下的农户耕地流转量预测分析

    Institute of Scientific and Technical Information of China (English)

    丁敬磊; 刘光远

    2014-01-01

    基于不同假设条件和目标定位,运用实证研究和计量预测分析的方法,构建研究区农地适度经营规模测度数学模型,分阶段对研究区农户耕地流转趋势、流转量、流转率和实现预测流转率需转移的农村隐性剩余劳动力数量进行计量预测,通过预测数据与实际数据的比较,找出假设条件对本地耕地流转产生的影响程度,揭示出研究区耕地流转缓慢的根本原因,以便有针对性地制定应对之策,最大限度提高耕地的生产潜力,促进农业现代化进程。研究结果表明,在阶段2和阶段3,农户耕地可实现较为稳定而高比率的流转;而在阶段1,农户耕地只能在低比率的情况下缓慢流转,耕地流转无论从规模还是利用效率上,都存在着巨大的可流转潜力。结果表明研究区实现农户耕地适度规模经营,需要转移就业的农业劳动力数量巨大、形势紧迫、任务艰巨。%Using the method of empirical research and quantitative analysis prediction based on different assumptions and targets , the mathematical model of agricultural land proper scale management was constructed .The cultivated land transfer , circulation amount , turnover rate and the number of rural surplus labor transfer prediction of hidden rate to be transferred in Yuncheng County were forecasted by stages .Then by comparing the predicted data and the actual data , the influence of local assumptions on the cultivated land transfer was found out , the root causes of slow flow of cultivated land in Yuncheng County was revealed , and then the coping strategies were developed in order to maximize the arable land production potential and to promote the process of agricultural modernization .The results showed that, in phase 2 and phase 3, it was possible to realize the stable and high turnover rate of the cultivated land;however, the cultivated land transferred slowly at low ratios in stage 1

  9. 通过设计型课程培养学生创新能力%Cultivation of Creative Ability in the Problem-based Curriculum

    Institute of Scientific and Technical Information of China (English)

    赵刚

    2011-01-01

    本文将电气工程专业课程分成授课型和设计型两类,讨论二者的联系与区别,以及各自在培养电气工程学生工作能力和创新能力方面的作用。授课型课程为学生建立宽厚的教育根基,是培养学生创新能力的前提条件。设计型课程鼓励学生主动学习,促进创新实践,是培养大学生工作与创新能力的载体。本文在明确当前大学生状况和教师职责的基础上,提出了一个旨在培养学生创新能力、基于开放性设计实践的设计型课程启发式教学流程。%The professional curriculums of the electrical engineering are divided into to classes: conventional lecture and problem-based curriculum. The difference and relationship are discussed in depth. The emphasis is placed on their functions on the cultivation of creative ability of the electrical engineering students respectively. The conventional lecture helps students to make concrete engineering education base, and is comer stone for the cultivation of creative capability, Problem-based curriculums can promote students to learn actively and make creative practice, and serves as the platform to foster the creative ability of the college students. On the analysis of the role of the college teachers and the status of the students, the heuristic teaching procedure of problem-based curriculum is proposed, whose aim is to cultivate the creative ability in the problem-based curriculum with open design project.

  10. Research on BOM based composable modeling method

    NARCIS (Netherlands)

    Zhang, M.; He, Q.; Gong, J.

    2013-01-01

    Composable modeling method has been a research hotpot in the area of Modeling and Simulation for a long time. In order to increase the reuse and interoperability of BOM based model, this paper put forward a composable modeling method based on BOM, studied on the basic theory of composable modeling m

  11. Exploration of Cultivation Model for Project-researching Based Logistics Talents%项目研究型物流人才培养模式探索

    Institute of Scientific and Technical Information of China (English)

    郑宁; 孙有望

    2013-01-01

    伴随物流业如火如荼的发展态势,物流人才“就业难”和企业“招工难”的结构性矛盾日益突出.提出了项目研究型物流人才创新培养模式,通过构建“三平台”的物流管理专业课程体系和“四层次”的物流管理专业实践教学环节进行学科建设和人才培养模式的创新实践,突出“专、通、雅”协调发展的物流人才培养特色.%In this paper we proposed the innovated mode for cultivating the project-researching based logistics talents,and through establishing the tri-platform of the logistics management specialty curricular system and the quad-layered logistics management specialty practical teaching link,put the innovated discipline construction and talent training mode into practice,which emphasized the logistics talent cultivation approach as characterized by professionalism,universalism and elegance.

  12. 以能力培养为主线建设实验教学示范中心%Constructing Experimental Teaching Demonstration Center Based on Ability Cultivation

    Institute of Scientific and Technical Information of China (English)

    温武; 黄立胜; 郭旭鸣; 丘凯伦

    2011-01-01

    介绍计算机科学与工程实验教学示范中心以培养满足社会需求的复合型和创新型计算机类专业人才为目标,充分利用优越的实验条件,发挥现有师资力量,将实验教学与实践相结合,建立以能力培养为主线的"一体化、分阶段、多层次"的独立实验课程教学体系.构造"特色课程"模块,培养学生科学素养、创新意识和协作精神,开拓学生探索科学潜能的创新思路,取得了显著的成效.%To cultivate complex and innovative computer professionals, the computer science and technology experimental teaching demonstration center makes full use of favorable experimental conditions and the existing teaching staff, combines experimental teaching with practice to set up ability based experimental teaching system, which is characterized by "integration,multi-phase,multi-level". The center also constructs "special courses" module to cultivate students' scientific quality,innovation consciousness and team spirit,to develop students' exploration potential. It has achieved remarkable results.

  13. Statistical evaluation and modeling of cheap substrate-based cultivation medium of Chlorella vulgaris to enhance microalgae lipid as new potential feedstock for biolubricant.

    Science.gov (United States)

    Mohammad Mirzaie, M A; Kalbasi, M; Mousavi, S M; Ghobadian, B

    2016-05-18

    Chlorella vulgaris (C. vulgaris) microalga was investigated as a new potential feedstock for the production of biodegradable lubricant. In order to enhance microalgae lipid for biolubricant production, mixotrophic growth of C. vulgaris was optimized using statistical analysis of Plackett-Burman (P-B) and response surface methodology (RSM). A cheap substrate-based medium of molasses and corn steep liquor (CSL) was used instead of expensive mineral salts to reduce the total cost of microalgae production. The effects of molasses and CSL concentration (cheap substrates) and light intensity on the growth of microalgae and their lipid content were analyzed and modeled. Designed models by RSM showed good compatibility with a 95% confidence level when compared to the cultivation system. According to the models, optimal cultivation conditions were obtained with biomass productivity of 0.123 g L(-1) day(-1) and lipid dry weight of 0.64 g L(-1) as 35% of dry weight of C. vulgaris. The extracted microalgae lipid presented useful fatty acid for biolubricant production with viscosities of 42.00 cSt at 40°C and 8.500 cSt at 100°C, viscosity index of 185, flash point of 185°C, and pour point of -6°C. These properties showed that microalgae lipid could be used as potential feedstock for biolubricant production.

  14. Application of Reduced Corn Cultivation Technology in Agro-Ecosystem of Cazin Municipality

    OpenAIRE

    Mirsad Veladžić; Fatima Muhamedagić; Emdžad Galijašević

    2011-01-01

    Intensive corn cultivation is predominant in current agriculture of the Una-Sana Canton. One of the corn cultivation methods in agro-ecosystem is reduced cultivation. The paper presents the experiment of “Osmak žuti” (eight-row yellow) corn cultivation on two control sites with application of reduced and intensive cultivation in Cazin municipality. The objectives of this research were to examine the possibility of application of reduced corn cultivation; analyze statistical variation elements...

  15. 讨论式学习在麻醉住院医师培养中的应用体会%The application of discussion learning method in the cultivation of anesthetic residents

    Institute of Scientific and Technical Information of China (English)

    姚立农

    2011-01-01

    It's essential to investigate an effective learning method in order to cultivate qualified anesthetic residents Discussion learning method has the characteristics of purposiveness and systematicness, and can promote learning activeness and cultivate creativity in clinical traning.The apllication of discussion learning method depend on the topic,preparation,style of discussion.In addition,the discussion should be guided and enlightenad by experienced senior doctors and a definite conclusion must be made after discussion%讨论式学习具有很强的针对性、系统性,而且能够调动学生学习积极性、培养其创新思维.精心确定讨论主题,讨论前充分准备,采用多形式讨论方式,及时地引导启发和总结是讨论式学习顺利实施的关键.

  16. Spirulina cultivation in China

    Science.gov (United States)

    Wu, Bo-Tang; Xiang, Wen-Zhou; Zeng, Cheng-Kui

    1998-03-01

    This paper reviews and discusses the development and many problems of Spirulina cultivation in China, points out the advantages and disadvantages of open photobioreactor system, and predicts that seawater Spirulina cultivation will be a new trend to be strengthened and emphasized due to its special physiological characteristics, easier management, lower fertilizer cost, and higher resistance to contaminants and rare pollution of chemicals.

  17. 区域耕地粮食生产保障能力及其风险评价方法%Risk assessment method of grain production guarantee capacity of regional cultivated land

    Institute of Scientific and Technical Information of China (English)

    季翔; 刘黎明; 起晓星

    2014-01-01

    区域耕地粮食生产保障能力是区域耕地对综合食物安全所需粮食的保障程度,受区域耕地资源和社会经济等方面的影响。科学评价区域耕地的粮食生产保障能力及其存在风险,对于解决综合食物安全问题起着关键性的作用。该文以区域耕地的粮食供给能力和粮食生产安全量预测为基础,建立了区域耕地粮食生产保障风险的评价方法,并以洞庭湖区为例开展了以县(市、区)为基本单元的实证研究。结果表明:洞庭湖区耕地资源对全国综合食物安全的保障情况较好,但部分县(市、区)的风险等级较高,且临湖区域的风险较相对远离洞庭湖区域的风险高,需要提高粮食生产水平并控制耕地非农化。该文所提出的区域耕地粮食生产保障能力及其风险评价方法为研究和评估不同区域粮食安全状况与耕地资源之间的相互关系提供参考。%Food security always attracts strong attention from researchers and policy makers in China, because it has more than one fifth of the world’s population, but the total cultivated area accounts for only about seven percent of the world’s total cultivated area. However, relevant studies were normally conducted at the national level, which generally considered China’s food security as a whole or used a single index system set for analysis. Therefore, study on food security from a regional scale will be of great value for policy makers. Grain production guarantee capacity of regional cultivated land is the satisfaction degree of regional grain supply to meet the national grain demand, which is mainly influenced by local cultivated land resources, cropping systems, and socio-economic factors. Regional total grain demand should be measured from a national perspective because of the mobility of grain and population;more specifically, it should be calculated based on the proportion of regional resource endowments

  18. Satellite-based investigation of flood-affected rice cultivation areas in Chao Phraya River Delta, Thailand

    Science.gov (United States)

    Son, N. T.; Chen, C. F.; Chen, C. R.; Chang, L. Y.

    2013-12-01

    The occurrence of catastrophic floods in Thailand in 2011 caused significant damage to rice agriculture. This study investigated flood-affected rice cultivation areas in the Chao Phraya River Delta (CRD) rice bowl, Thailand using time-series moderate resolution imaging spectroradiometer (MODIS) data. The data were processed for 2008 (normal flood year) and 2011, comprising four main steps: (1) data pre-processing to construct time-series MODIS vegetation indices (VIs), to filter noise from the time-series VIs by the empirical mode decomposition (EMD), and to mask out non-agricultural areas in respect to water-related cropping areas; (2) flood-affected area classification using the unsupervised linear mixture model (ULMM); (3) rice crop classification using the support vector machines (SVM); and (4) accuracy assessment of flood and rice crop mapping results. The comparisons between the flood mapping results and the ground reference data indicated an overall accuracy of 97.9% and Kappa coefficient of 0.62 achieved for 2008, and 95.7% and 0.77 for 2011, respectively. These results were reaffirmed by close agreement (R2 > 0.8) between comparisons of the two datasets at the provincial level. The crop mapping results compared with the ground reference data revealed that the overall accuracies and Kappa coefficients obtained for 2008 were 88.5% and 0.82, and for 2011 were 84.1% and 0.76, respectively. A strong correlation was also found between MODIS-derived rice area and rice area statistics at the provincial level (R2 > 0.7). Rice crop maps overlaid on the flood-affected area maps showed that approximately 16.8% of the rice cultivation area was affected by floods in 2011 compared to 4.9% in 2008. A majority of the flood-expanded area was observed for the double-cropped rice (10.5%), probably due to flood-induced effects to the autumn-summer and rainy season crops. Information achieved from this study could be useful for agricultural planners to mitigate possible impacts

  19. Instance Based Methods --- A Brief Overview

    CERN Document Server

    Baumgartner, Peter; 10.1007/s13218-010-0002-x

    2012-01-01

    Instance-based methods are a specific class of methods for automated proof search in first-order logic. This article provides an overview of the major methods in the area and discusses their properties and relations to the more established resolution methods. It also discusses some recent trends on refinements and applications. This overview is rather brief and informal, but we provide a comprehensive literature list to follow-up on the details.

  20. 基于育人为本与能力培养为核心的教学设计与实践%Teaching design and practice based on the core of cultivation-orientation and ability training

    Institute of Scientific and Technical Information of China (English)

    张子贤

    2016-01-01

    课程建设与改革是提高教学质量的核心,基于育人为本与能力培养为核心的教学设计对提高人才培养质量具有重要的意义。阐述了基于育人为本与能力培养为核心的课程整体设计和教学设计。课程整体设计主要包括学习任务、教学模式、实现知识与能力目标的路径等方面的设计;教学设计论述了以理论支撑技术应用能力的理实一体化、思想、方法与细节的“三位一体”、教法引领学法、重视非智力因素等方面的设计。笔者将上述设计付诸于教学实践,收到了良好效果。%The course construction and reform is the core of improving teaching quality. The teaching design based on the core of cultivation-orientation and ability training has important sig-nificance to improve the quality of talents cultivation.This paper expands the overall design and teaching design based on the core of cultivation-orientation and ability training.The overall de-sign of the course indudes design of the learning task,the teaching mode,the path of realizing the goal of knowledge and ability.The teaching design discusses the trinity of integration of the-ory and practice,thinking,method and detail with technology application ability supported by theories,teaching guide learning methods,the importance of non-intelligence factor. We puts the above design into teaching practice,receives a good effect.

  1. [The Theory and Practice of Health Cultivation Qigong Exercise in Traditional Chinese Medicine].

    Science.gov (United States)

    Chang, Mei-Ying

    2015-12-01

    The health cultivation qigong exercise in traditional Chinese medicine refers to a traditional, integrated method of illness prevention and body strengthening, which promotes the functions of qi and the blood, smooths the meridians (energy channels), and balances the viscera and bowels through the regulation of the mind, the breathing, and the body. The concept of using qi to cultivate human life is part of the health cultivation practices of ancient Chinese codes and of Chinese medicine. This concept includes the principles, methods, essences, and clinical applications of the practice. In addition, traditional health cultivation references the concepts of yinyang, viscera and bowels, qi and blood, meridians, and essential energy spirit theory in order to explain the human biological phenomena, the theoretical and practical perspectives of qigong, and the basis of the treatment principle. The health cultivation qigong exercise of Chinese medicine utilizes the concept of the "unity of nature and human beings" in traditional Chinese thinking in its practice, which emphasizes the conformity to nature and seasons. In order to fully leverage the benefits from the purpose of health cultivation in qigong practice, the priority is to understand the health cultivation mechanism, the essentials/matters, and the precautions of qigong practices. Recently, the evidence regarding both the biological and the psychological benefits of qigong practices have been demonstrated in numbers of research articles. In particular, qigong is currently considered to be one of the best mild exercises that is suited to all age groups. Professional nurses are suggested to include the health cultivation qigong exercise as part of activities that target health improvement and illness prevention. Due to the diversity in qigong as practiced by different health cultivation qigong exercise sects, it is essential to accumulate more clinical evidence by conducting greater numbers of rigorous studies

  2. Reliability-based concurrent subspace optimization method

    Institute of Scientific and Technical Information of China (English)

    FAN Hui; LI Wei-ji

    2008-01-01

    To avoid the high computational cost and much modification in the process of applying traditional re-liability-based design optimization method, a new reliability-based concurrent subspace optimization approach is proposed based on the comparison and analysis of the existing muhidisciplinary optimization techniques and reli-ability assessment methods. It is shown through a canard configuration optimization for a three-surface transport that the proposed method is computationally efficient and practical with the least modification to the current de-terministic optimization process.

  3. Influence of carvacrol and 1,8-cineole on cell viability, membrane integrity, and morphology of Aeromonas hydrophila cultivated in a vegetable-based broth.

    Science.gov (United States)

    de Sousa, Jossana Pereira; de Oliveira, Kataryne Árabe Rimá; de Figueiredo, Regina Celia Bressan Queiroz; de Souza, Evandro Leite

    2015-02-01

    This study investigated the effects of carvacrol (CAR) and 1,8-cineole (CIN) alone (at the MIC) or in combination at subinhibitory amounts (both at 1/8 MIC) on the cell viability, membrane permeability, and morphology of Aeromonas hydrophila INCQS 7966 (A. hydrophila) cultivated in a vegetable-based broth. CAR and CIN alone or in combination severely affected the viability of the bacteria and caused dramatic changes in the cell membrane permeability, leading to cell death, as observed by confocal laser microscopy. Scanning and transmission electron microscopy images of bacterial cells exposed to CAR or CIN or the mixture of both compounds revealed severe changes in cell wall structure, rupture of the plasma membrane, shrinking of cells, condensation of cytoplasmic content, leakage of intracellular material, and cell collapse. These findings suggest that CAR and CIN alone or in combination at subinhibitory amounts could be applied to inhibit the growth of A. hydrophila in foods, particularly as sanitizing agents in vegetables.

  4. 浙江省西红花“二段法”优质高产栽培技术研究%High-quality and High-yield Cultivation Technique of Two Segments Method for Crocus sativus L.in Zhejiang Province

    Institute of Scientific and Technical Information of China (English)

    饶君凤; 王根法; 吕伟德

    2012-01-01

    通过西红花栽培研究试验及示范推广,作者对西红花的药用价值、特征特性、栽培技术、田间管理、病虫害防治及采收加工进行了系统的研究,提出浙江省西红花“二段法”栽培的技术措施.西红花在浙江高产栽培的关键是选用8g以上的种球,11月上中旬田间露地种植,在科学的肥水运筹下越冬生长并发育形成新的子球,翌年5月植株休眠将商品用球采收放到室内通风阴湿的环境下催芽,10月底到11月采花.这套技术的推广应用为浙江省西红花产业发展及栽培管理提供了科学依据.%Based on the cultivation test and demonstration, the characteristics, medicinal value, cultivation technique, field management, pest control, harvest and process of Crocus sativus L. Were analyzed systemically. Two Segments Method for the cultivation of C. Sativus in Zhejiang Province was put forward. Selecting bulbs above 8 g was the key for high-yield cultivation of C. Sativus in Zhejiang Province. In early November, C. Sativus was cultivated in the open field, and grew over winter under scientific application of fertilizer and water. In the following May, bulbs were collected in ventilating, shady and humid room to accelerate germination. Flowers were collected from late October to November. Popularization of this matching technique provided scientific guidance for the industrial development and cultivation management of C. Sativus.

  5. DNA-based taxonomic identification of basidiospores in hallucinogenic mushrooms cultivated in "grow-kits" seized by the police: LC-UV quali-quantitative determination of psilocybin and psilocin.

    Science.gov (United States)

    Gambaro, Veniero; Roda, Gabriella; Visconti, Giacomo Luca; Arnoldi, Sebastiano; Casagni, Eleonora; Dell'Acqua, Lucia; Farè, Fiorenza; Paladino, Eleonora; Rusconi, Chiara; Arioli, Stefania; Mora, Diego

    2016-06-01

    The taxonomic identification of the biological material contained in the hallucinogenic mushrooms culture media, was carried out using a DNA-based approach, thus highlighting the usefulness of this approach in the forensic identification of illegal samples also when they are present as basidiospores mixed in culture media and spore-bearing fruiting body are not present. This approach is very useful as it allows the unequivocal identification of potentially illicit material before the cultivation and it enables to stop the material to the Customs and to destroy it due to its dangerousness without cultivating the "grow-kits" and without instructing a criminal case. In fact, even if psilocin and psilocybin and the whole mushrooms are illegal in many countries, there is no specific indication in the law about the so called "grow-kits", containing the spores. To confirm the data obtained by the taxonomic identification, a simple, reliable, efficient LC-UV method, using tryptamine as internal standard, suitable for the forensic quali-quantitative determination of psilocin and psilocybin in hallucinogenic mushroom was optimized, validated and applied to the mushrooms grown after the cultivation of the grow-kits seized by the judicial authority, with the authorization of the Ministry of Health. A cation exchange column was used in a gradient elution mode (Phase A: 50mMK2HPO4; 100mM NaCl pH=3 Phase B: methanol). The developed method was linear over the calibration range with a R(2)>0.9992 for both the analytes. The detection and quantification limits were respectively 0.01 and 0.1μg/mL for psilocybin and 0.05μg/mL and 0.1μg/mL for psilocin and the intra- and inter-day precision was satisfactory (coefficients of variation psilocybin in the mushrooms grown from the seized "grow-kits" ranged from 1.02 to 7.60mg/g of dry vegetable material, while the content of psilocin from 0.415 to 8.36mg/g.

  6. Decision making based on data analysis methods

    OpenAIRE

    Sirola, Miki; Sulkava, Mika

    2016-01-01

    This technical report is based on four our recent articles:"Data fusion of pre-election gallups and polls for improved support estimates", "Analyzing parliamentary elections based on voting advice application data", "The Finnish car rejection reasons shown in an interactive SOM visualization tool", and "Network visualization of car inspection data using graph layout". Neural methods are applied in political and technical decision making. We introduce decision support schemes based on Self-Org...

  7. Determination of total saccharide in cultivated Dictyophora indusia Fisscher of Hainan by spectrophotometric method%海南栽培长裙竹荪中总糖含量的测定

    Institute of Scientific and Technical Information of China (English)

    莫峥嵘; 陈光英; 古翰林; 宋鑫明; 陈喜蓉

    2012-01-01

    To determine total saccharide in cultivated Dictyophora indusia Fisscher of Hainan.Under the experimental condition including glucose as the reference,5% phenol-sulphuric acid as color developer.The content of total saccharide in cultivated Dictyophora indusia Fisscher of Hainan was determined by spectrophotometric method at 490 nm.The calibration curve was linear(R2=0.9960) in the range of 0.01~0.08 mg/mL and the curve was Y=-0.005+10.38333X.The content of total saccharide in cultivated Dictyophora indusia Fisscher of Hainan was 8.16%.The spectrophotometric method was suitable for the determination of total saccharide in cultivated Dictyophora indusia Fisscher of Hainan.This method is convenient,stable and repeatable.%测定海南栽培长裙竹荪中总糖的含量。以葡萄糖为标准品,5%苯酚溶液和浓硫酸为显色剂,采用分光光度法在490nm波长处测定样品的吸光度。葡萄糖标准品在(0.01~0.08mg/mL)范围内线性关系良好,其回归方程Y=-0.005+10.38333X,R2=0.9969。海南栽培长裙竹荪中总糖的含量为8.16%。分光光度法适合长裙竹荪中总糖的含量测定,该方法操作简便,结果相对稳定,重现性好。

  8. Advancing gut microbiome research using cultivation

    DEFF Research Database (Denmark)

    Sommer, Morten OA

    2015-01-01

    Culture-independent approaches have driven the field of microbiome research and illuminated intricate relationships between the gut microbiota and human health. However, definitively associating phenotypes to specific strains or elucidating physiological interactions is challenging for metagenomic...... approaches. Recently a number of new approaches to gut microbiota cultivation have emerged through the integration of high-throughput phylogenetic mapping and new simplified cultivation methods. These methodologies are described along with their potential use within microbiome research. Deployment of novel...... cultivation approaches should enable improved studies of xenobiotic tolerance and modification phenotypes and allow a drastic expansion of the gut microbiota reference genome catalogues. Furthermore, the new cultivation methods should facilitate systematic studies of the causal relationship between...

  9. Molecular- and cultivation-based analyses of microbial communities in oil field water and in microcosms amended with nitrate to control H{sub 2}S production

    Energy Technology Data Exchange (ETDEWEB)

    Kumaraswamy, Raji; Ebert, Sara; Fedorak, Phillip M.; Foght, Julia M. [Alberta Univ., Edmonton, AB (Canada). Biological Sciences; Gray, Murray R. [Alberta Univ., Edmonton, AB (Canada). Chemical and Materials Engineering

    2011-03-15

    Nitrate injection into oil fields is an alternative to biocide addition for controlling sulfide production ('souring') caused by sulfate-reducing bacteria (SRB). This study examined the suitability of several cultivation-dependent and cultivation-independent methods to assess potential microbial activities (sulfidogenesis and nitrate reduction) and the impact of nitrate amendment on oil field microbiota. Microcosms containing produced waters from two Western Canadian oil fields exhibited sulfidogenesis that was inhibited by nitrate amendment. Most probable number (MPN) and fluorescent in situ hybridization (FISH) analyses of uncultivated produced waters showed low cell numbers ({<=}10{sup 3} MPN/ml) dominated by SRB (>95% relative abundance). MPN analysis also detected nitrate-reducing sulfide-oxidizing bacteria (NRSOB) and heterotrophic nitrate-reducing bacteria (HNRB) at numbers too low to be detected by FISH or denaturing gradient gel electrophoresis (DGGE). In microcosms containing produced water fortified with sulfate, near-stoichiometric concentrations of sulfide were produced. FISH analyses of the microcosms after 55 days of incubation revealed that Gammaproteobacteria increased from undetectable levels to 5-20% abundance, resulting in a decreased proportion of Deltaproteobacteria (50-60% abundance). DGGE analysis confirmed the presence of Delta- and Gammaproteobacteria and also detected Bacteroidetes. When sulfate-fortified produced waters were amended with nitrate, sulfidogenesis was inhibited and Deltaproteobacteria decreased to levels undetectable by FISH, with a concomitant increase in Gammaproteobacteria from below detection to 50-60% abundance. DGGE analysis of these microcosms yielded sequences of Gamma- and Epsilonproteobacteria related to presumptive HNRB and NRSOB (Halomonas, Marinobacterium, Marinobacter, Pseudomonas and Arcobacter), thus supporting chemical data indicating that nitrate-reducing bacteria out-compete SRB when nitrate is

  10. 基于4C需求的网球裁判技能培养探讨%Cultivation of Tennis Umpire Based on the 4C Skills

    Institute of Scientific and Technical Information of China (English)

    徐祥峰; 王海明

    2012-01-01

    现代网球运动项目存在职业化和商业化的发展趋势,技战术存在快速、灵活、多变与对抗激烈的特点,这对网球裁判的执裁能力提出了更高的要求。文章探讨网球裁判技能的"4C"要求,即Commonsense(常识)、Communication(沟通)、Control(控制)、Concentration(专注),分析其内涵及外延,提出具体的培养要求和方法。文章将丰富我国网球裁判培训的理论知识体系,同时为裁判培养工作提供重要的理论依据和实践指导。%Tennis has become much more professionalized and commercialized in recent years,which has the technical and tactical features of quickness,flexibility and variability.And there are fierce competitions between opponents as well.Under the developing background,tennis competitions put forward higher requests to the quality of tennis umpire in judging process.This article explores the skill model of tennis umpire,including commonsense,communication,control and concentration,called "4C" for short,and probes into their particular connotation and denotation.At the same time,the thesis advances requirements and methods for cultivating tennis umpire of high quality.This research could not only enrich relative theories of cultivation,but also provide theoretical foundation and practical direction for training of tennis umpire.

  11. Cultivation-Based and Molecular Assessment of Bacterial Diversity in the Rhizosheath of Wheat under Different Crop Rotations.

    Directory of Open Access Journals (Sweden)

    Muhammad Tahir

    Full Text Available A field study was conducted to compare the formationand bacterial communities of rhizosheaths of wheat grown under wheat-cotton and wheat-rice rotation and to study the effects of bacterial inoculation on plant growth. Inoculation of Azospirillum sp. WS-1 and Bacillus sp. T-34 to wheat plants increased root length, root and shoot dry weight and dry weight of rhizosheathsoil when compared to non-inoculated control plants, and under both crop rotations. Comparing both crop rotations, root length, root and shoot dry weight and dry weight of soil attached with roots were higher under wheat-cotton rotation. Organic acids (citric acid, malic acid, acetic acid and oxalic acid were detected in rhizosheaths from both rotations, with malic acid being most abundant with 24.8±2 and 21.3±1.5 μg g(-1 dry soil in wheat-cotton and wheat-rice rotation, respectively. Two sugars (sucrose, glucose were detected in wheat rhizosheath under both rotations, with highest concentrations of sucrose (4.08±0.5 μg g(-1 and 7.36±1.0 μg g(-1 and glucose (3.12±0.5 μg g(-1 and 3.01± μg g(-1 being detected in rhizosheaths of non-inoculated control plants under both rotations. Diversity of rhizosheath-associated bacteria was evaluated by cultivation, as well as by 454-pyrosequencing of PCR-tagged 16S rRNA gene amplicons. A total of 14 and 12 bacterial isolates predominantly belonging to the genera Arthrobacter, Azospirillum, Bacillus, Enterobacter and Pseudomonaswere obtained from the rhizosheath of wheat grown under wheat-cotton and wheat-rice rotation, respectively. Analysis of pyrosequencing data revealed Proteobacteria, Bacteriodetes and Verrucomicrobia as the most abundant phyla in wheat-rice rotation, whereas Actinobacteria, Firmicutes, Chloroflexi, Acidobacteria, Planctomycetes and Cyanobacteria were predominant in wheat-cotton rotation. From a total of 46,971 sequences, 10.9% showed ≥97% similarity with 16S rRNA genes of 32 genera previously shown to include

  12. Human mesenchymal stem cell expression program upon extended ex-vivo cultivation, as revealed by 2-DE-based quantitative proteomics.

    Directory of Open Access Journals (Sweden)

    Andreia Madeira

    Full Text Available Human mesenchymal stem cells (MSC have been on the focus of intense clinical-oriented research due to their multilineage differentiation potential and immunomodulatory properties. However, to reach the clinically meaningful cell numbers for cellular therapy and tissue engineering applications, MSC ex-vivo expansion is mandatory but sequential cell passaging results in loss of proliferative, clonogenic and differentiation potential. To get clues into the molecular mechanisms underlying cellular senescence resulting from extended ex-vivo cultivation of bone marrow (BM MSC, we explored a two-dimensional gel electrophoresis (2-DE based quantitative proteomics to compare the expression programs of Passage 3 cells (P3, commonly used in clinical studies with expanded MSC, and Passage 7 (P7 cells, which already demonstrated significant signs of culture-induced senescence. Proteins of the functional categories "Structural components and cellular cytoskeleton" and "Folding and stress response proteins" are less abundant in P7 cells, compared to P3, while proteins involved in "Energy metabolism", "Cell cycle regulation and aging" and "Apoptosis" are more abundant. The large number of multiple size and charge isoforms with an altered content that were identified in this study in P7 versus P3, namely the cytoskeleton components β-actin (7 forms and vimentin (24 forms, also emphasizes the importance of post-transcriptional modification upon long-term cultivation. The differential protein expression registered suggests that cellular senescence occurring during ex-vivo expansion of BM MSC is associated with the impairment of cytoskeleton remodeling and/or organization and the repair of damaged proteins resulting from cell exposure to culture stress. The genome-wide expression approach used in this study has proven useful for getting mechanistic insights into the observed decrease on the proliferative and clonogenic potential of P7 versus P3 cells and paves the

  13. Human mesenchymal stem cell expression program upon extended ex-vivo cultivation, as revealed by 2-DE-based quantitative proteomics.

    Science.gov (United States)

    Madeira, Andreia; da Silva, Cláudia L; dos Santos, Francisco; Camafeita, Emilio; Cabral, Joaquim M S; Sá-Correia, Isabel

    2012-01-01

    Human mesenchymal stem cells (MSC) have been on the focus of intense clinical-oriented research due to their multilineage differentiation potential and immunomodulatory properties. However, to reach the clinically meaningful cell numbers for cellular therapy and tissue engineering applications, MSC ex-vivo expansion is mandatory but sequential cell passaging results in loss of proliferative, clonogenic and differentiation potential. To get clues into the molecular mechanisms underlying cellular senescence resulting from extended ex-vivo cultivation of bone marrow (BM) MSC, we explored a two-dimensional gel electrophoresis (2-DE) based quantitative proteomics to compare the expression programs of Passage 3 cells (P3), commonly used in clinical studies with expanded MSC, and Passage 7 (P7) cells, which already demonstrated significant signs of culture-induced senescence. Proteins of the functional categories "Structural components and cellular cytoskeleton" and "Folding and stress response proteins" are less abundant in P7 cells, compared to P3, while proteins involved in "Energy metabolism", "Cell cycle regulation and aging" and "Apoptosis" are more abundant. The large number of multiple size and charge isoforms with an altered content that were identified in this study in P7 versus P3, namely the cytoskeleton components β-actin (7 forms) and vimentin (24 forms), also emphasizes the importance of post-transcriptional modification upon long-term cultivation. The differential protein expression registered suggests that cellular senescence occurring during ex-vivo expansion of BM MSC is associated with the impairment of cytoskeleton remodeling and/or organization and the repair of damaged proteins resulting from cell exposure to culture stress. The genome-wide expression approach used in this study has proven useful for getting mechanistic insights into the observed decrease on the proliferative and clonogenic potential of P7 versus P3 cells and paves the way to set

  14. Software Testing Method Based on Model Comparison

    Institute of Scientific and Technical Information of China (English)

    XIE Xiao-dong; LU Yan-sheng; MAO Cheng-yin

    2008-01-01

    A model comparison based software testing method (MCST) is proposed. In this method, the requirements and programs of software under test are transformed into the ones in the same form, and described by the same model describe language (MDL).Then, the requirements are transformed into a specification model and the programs into an implementation model. Thus, the elements and structures of the two models are compared, and the differences between them are obtained. Based on the diffrences, a test suite is generated. Different MDLs can be chosen for the software under test. The usages of two classical MDLs in MCST, the equivalence classes model and the extended finite state machine (EFSM) model, are described with example applications. The results show that the test suites generated by MCST are more efficient and smaller than some other testing methods, such as the path-coverage testing method, the object state diagram testing method, etc.

  15. Comparison of Spatial Interpolation Methods for Soil Nutrients in Cultivated Land Fertility Evaluation%县域耕地地力评价中土壤养分空间插值方法的比较研究

    Institute of Scientific and Technical Information of China (English)

    李增兵; 赵庚星; 赵倩倩; 李百红; 武婕

    2012-01-01

    耕地是农业生产的重要资源,耕地地力评价是耕地质量建设的重要手段,与之相关的土壤养分插值技术关系到耕地地力评价精度与可用性.以历城区为研究对象,将历城区划分为历城平原区和丘陵区,分析其采样点养分分布情况、评价指标中涉及的土壤有机质、有效磷、速效钾、有效锌、有效硼的空间变异情况.分别用反距离权重插值法(IDW)和普通克里格插值法(OK)对历城全区、平原区和丘陵区的土壤养分进行插值,通过交叉验证、叠加对比分析等方法比较不同情况下不同插值方法的精度,并对比分析基于不同插值方法的耕地地力评价结果.结果显示,IDW和OK2种方法都具有较高精度,其中IDW插值方法较适宜于丘陵区,而OK插值方法则适宜于平原区;对于有多种参评元素参与的县域耕地地力评价,采用2种方法的评价结果相差不大.基于实用性与精确性结合原则,IDW方法是县域耕地地力评价养分空间内插的最佳选择.%Farmland was an important resource of agricultural production, and cultivated land fertility evaluation is an important guarantee for quality construction of arable land, soil nutrients interpolation techniques affect the evaluation of farmland accuracy and availability immediately. This paper took data of Licheng County as an example, the city was classified into plain and hilly region based on the landform condition. We analyzed distribution and spatial variation via the sample point data, including organic matter, available phosphorus, rapid available potassium, available zinc and available boron. This paper carried on interpolation of soil nutrients by the model of Inverse Distance Weighted and Ordinary Kriging in Licheng plain and hilly regions. Moreover, we compared the two kinds of interpolation method by the way of cross-validation and comparative analysis. At the same time, compared and analyzed the different

  16. 浅谈培养学生音乐记忆力的方法与途径%A Brief Discussion on the Methods and Approaches of Cultivating Students' Music Memory

    Institute of Scientific and Technical Information of China (English)

    张建芬

    2013-01-01

    音乐记忆力指的是对音乐作品的记忆能力,也是我们学生学习音乐必须具备的基本能力,正确的音乐记忆应遵循记忆的规律,讲究方法,在学习中加以培养。本文旨在如何培养学生良好的音乐记忆能力上做了一些尝试。%Music memory, students' basic ability in learning mu-sic, is the memorizing ability of music works. A correct music memory obeys the rules and methods of memory and should be cultivated in learning. This paper attempts to cultivate students' correct music memory ability.

  17. Cultivated Land Changes and Their Driving Forces-A Satellite Remote Sensing Analysis in the Yellow River Delta, China

    Institute of Scientific and Technical Information of China (English)

    ZHAO Geng-Xing; G.LIN; J.J.FLETCHER; C.YUILL

    2004-01-01

    Taking Kenli County in the Yellow River Delta, China, as the study area and using digital satellite remote sensing techniques, cultivated land use changes and their corresponding driving forces were explored in this study. An interactive interpretation and a manual modification procedure were carried out to acquire cultivated land information. An overlay method based on classification results and a visual change detection method which was supported by land use maps were employed to detect the cultivated land changes. Based on the changes that were revealed and a spatial analysis between cultivated land use and related natural and socio-economic factors, the driving forces for cultivated land use changes in the study area were determined.The results showed a decrease in cultivated land in Kenli County of 5321.8 ha from 1987 to 1998, i.e.,an average annual decrement of 483.8 ha, which occurred mainly in the central paddy field region and the northeast dry land region. Adverse human activities, soil salinization and water deficiencies were the driving forces that caused these cultivated land use changes.

  18. A study on the comparison of antioxidant effects among cultivated ginseng, and cultivated wild ginseng extracts -Using the measurement of superoxide and hydroxy radical scavenging activities-

    Directory of Open Access Journals (Sweden)

    Tae Jin, Rhim

    2009-06-01

    Full Text Available Objectives : The objective of this study was to compare the antioxidant effects among cultivated wild ginseng and ginseng extracts. Methods : In vitro antioxidant activities were examined by superoxide and hydroxyl radical scavenging activities of ginseng and cultivated wild ginseng extracts. Results : 1. In the superoxide radical scavenging activities of ginseng and cultivated wild ginseng extracts, antioxidant activities of cultivated wild ginseng extracts was showed higher than cultivated ginseng in the concentration of 0.25 and 0.50㎎/㎖. 2. In the hydroxyl radical scavenging activities of ginseng and cultivated wild ginseng extracts, antioxidant activities of cultivated wild ginseng extracts was showed higher than cultivated ginseng in the concentration of 1.0, 2.5, and 5.0㎎/㎖. Conclusions : In summary, the results of this study demonstrate that cultivated wild ginseng extracts had higher antioxidant activities to cultivated ginseng.

  19. 袁同礼的图书馆人才培植方法及启示%Yuan Tongli's Methods about Cultivating Talents of Libraries and Its Revelation

    Institute of Scientific and Technical Information of China (English)

    潘梅

    2011-01-01

    Yuan Tongli who is an important leader of Chinese librarianship in the period of the Republic of China, he has own unique ways of cultivating talents. In selecting talents, he attached importance to knowledge basis and academic skills of talents. In using talents, he respected talents' interests and took advantage of their strengths. In cultivating talents, he provided many opportunities of academic training and advanced studying for talents. In retaining talents, he cared them and fully played leadership charisma. Yuan Tongli's methods about cultivating talents have a great inspiration for libraries today. Firstly, cultivating talents of library is a strategic issue related to librarianship development and academic progress. Secondly, libraries should moderately increase the space-level positions in favor to cultivate talents. Finally, libraries should pay attention to the establishment of long-term mechanism of cultivating talents.%民国时期中国图书馆事业的重要领导者袁同礼在培植图书馆人才上有自己独特的方法:在选才方面,重视人才的知识基础和学术功底;在用才方面,尊重人才的志趣,用人所长;在育才方面,努力为人才提供学术训练和进修深造的机会,注重人才的成长;在留才方面,关心人才,充分发挥个人的领导魅力。此方法对当今图书馆的人才培植仍有极大的启发意义。图书馆人才培养是事关图书馆事业发展、学术进步的战略问题,图书馆应适度增加空间职位层次,建立长效的人才培养机制,为人才提供多方面的成长渠道。

  20. Wavelet-based Multiresolution Particle Methods

    Science.gov (United States)

    Bergdorf, Michael; Koumoutsakos, Petros

    2006-03-01

    Particle methods offer a robust numerical tool for solving transport problems across disciplines, such as fluid dynamics, quantitative biology or computer graphics. Their strength lies in their stability, as they do not discretize the convection operator, and appealing numerical properties, such as small dissipation and dispersion errors. Many problems of interest are inherently multiscale, and their efficient solution requires either multiscale modeling approaches or spatially adaptive numerical schemes. We present a hybrid particle method that employs a multiresolution analysis to identify and adapt to small scales in the solution. The method combines the versatility and efficiency of grid-based Wavelet collocation methods while retaining the numerical properties and stability of particle methods. The accuracy and efficiency of this method is then assessed for transport and interface capturing problems in two and three dimensions, illustrating the capabilities and limitations of our approach.

  1. The Research on High Yield Cultivation Technology of the High Starch Ipomoea Batatas Lam Based on Orthogonal Experiment%基于正交实验的高淀粉甘薯高产栽培技术研究

    Institute of Scientific and Technical Information of China (English)

    尤瑞玲; 李红忠; 史本林

    2012-01-01

    The ipomoea batatas lam is one of world staple food crops, also is the important industrial crop and the feed crops. In recent years, the domestic scholars have conducted the massive research to the high yield cultivation technique of the ipomoea batatas lam. In order to improve the yield of 'Shangshu 19', the article uses the orthogonal experiment method through combining the experiment, basing on the cultivation technology of ipomoea batatas lam in different transplanting time, planting density, ridge width, ridge height four factors. The article conducts the contrast experimental study for the ipomoea batatas lam, in order to select the best comprehensive technique measures and guide the field production. The results showed that, the five combinations got the highest yield. There were significant differences with other groups. The important cultivation and management measures were the timely plug, the proper ridge and the rational application of fertilizer.%甘薯是世界主要粮食作物之一,也是重要的工业原料作物和饲料作物.近年来,中国学者对甘薯高产栽培技术进行了大量研究.为了提高能源型‘商薯19’的产量,采用正交实验方法,通过对甘薯栽培技术中不同移栽时间、种植密度、起垄宽度、起垄高度4个因素进行组合试验,进行甘薯增产对比试验研究,以筛选出最佳综合配套技术措施方案,指导大田生产.结果表明:处理5组合的产量最高,与其他各组存在极显著差异.即适时早插,起垄适当,合理施肥是提高甘薯产量的重要栽培管理措施.

  2. Optimization of Streptomyces peucetius var. caesius N47 cultivation and epsilon-rhodomycinone production using experimental designs and response surface methods.

    Science.gov (United States)

    Kiviharju, K; Leisola, M; Eerikäinen, T

    2004-11-01

    Streptomyces peucetius var. caesius is an aerobic bacterium that produces doxorubicin as a secondary metabolite. A mixture design was applied for the screening of suitable complex medium components in the cultivation of S. peucetius var. caesius N47, which is an epsilon-rhodomycinone-accumulating mutant strain. epsilon-Rhodomycinone is a non-glycosylated precursor of doxorubicin. Best growth results were obtained with soy peptone and beef extract. A central composite face-centered (CCF) experimental design was constructed for the investigation of pH, temperature and dissolved oxygen (DO) effects on the cultivation growth phase. Another CCF was applied to the production phase to investigate the effects of aeration, pH, temperature and stirring rate on epsilon-rhodomycinone production. An increase in cultivation temperature increased both cell growth and glucose consumption rate. Best epsilon-rhodomycinone productivities were obtained in temperatures around 30 degrees C. DO control increased all growth phase responses, but aeration in the production phase coupled with pH decrease resulted in rapid epsilon-rhodomycinone decay in the medium. In non-aerated production phases a pH change resulted in better productivity than in experiments without pH change. A pH increase with a temperature decrease seemed most beneficial for productivity. This implies that dynamic control strategies in batch production of epsilon-rhodomycinone could increase the overall process productivity.

  3. Research on Talent Cultivation Objectives for College Physical Education Majors Based on Manage-ment By Objectives%基于“目标管理理论”的高师体育教育专业人才培养目标管理探析

    Institute of Scientific and Technical Information of China (English)

    沈强

    2014-01-01

    通过文献资料法、逻辑分析法,对我国高师体育教育专业人才培养目标进行了历史梳理和现状分析,结果认为:目前高师体育教育专业人才培养现状存在:人才就业导向现状与高师院校体育教育专业目标的不适应、人才培养的课程设置与高师院校体育教育专业目标的不适应、个人发展目标模糊与高师院校体育教育专业目标的不适应等问题。根据“目标管理理论”与存在的问题,提出了高师体育教育专业人才培养目标管理体系,并从理论依据、体系构建、实施路径、管理策略等方面对人才培养目标管理体系做了具体分析,为高师体育教育专业人才培养管理提供参考依据。%With the methods of documentary information and logic analysis ,based on the historical introduction and the analysis of present situation about talent cultivation objective for college physical education majors ,the survey result suggests that at present , the problems in talent cultivation objective for college physical education majors lie in following aspects :talent cultivation objective can not adapt itself to employment direction ;the curriculum can not agree with talent cultivation objectives ;vague personal develop-ment goals can not agree with talent cultivation objectives .According to Management By Objectives and existing problems ,the paper puts forwards talent cultivation objective management system for college physical education majors ,and analyzes the management system from aspects of theory basis ,system construction ,implement approach ,and management policy ,which provides some refer-ences for cultivating talents of college physical education majors .

  4. Spatial variability of available phosphorus for cultivated soil based on GARBF neural network%基于GARBF神经网络的耕地土壤有效磷空间变异分析

    Institute of Scientific and Technical Information of China (English)

    徐剑波; 宋立生; 夏振; 张桥; 胡月明

    2012-01-01

    correlation in surface cultivated soil AP was weak in the five sampling scales (training sample points were 100, 200, 300,400 and 500), while unobvious in wide range. Predictions of soil AP in simulation using GARBF neural network was better than that using radial basis function(RBF) neural network (Near-RBF) prediction model based on several closest neighbors and Ordinary Kriging method. In practical application, the spatial interpolation map by GARBF neural network method with 300 soil samples showed a serious trend of surplus phosphate in cropping in Gaozhou city. Diffusive surplus phosphorus made a serious threat to the water environment in this region. The results provide a theoretical basis and technical support for predicting soil property spatial distribution accurately, using fertilizer properly and mitigating non-point source pollution of water.

  5. A Space Flight Cultivation Protocol for Arabidopsis

    Science.gov (United States)

    Levine, H. G.

    2008-06-01

    A tube-based method is presented for the cultivation and manipulation of Arabidopsis thaliana during space flight experimentation. Seeds were germinated on rock-wool plugs and subsequently transferred into modified polypropylene conical tubes (cut to 5 cm lengths) at 7 days after planting. Each tube contained four side-situated slits through which capillary mat strips were woven. An additional capillary mat wick extended from below the tube up through the bottom to the mid-interior portion. The incorporation of Fibrous Ion Exchange Resin Substrate provided nutrients. The tubes were transferred to plant compartments containing a horticulture foam matrix that received water inputs. Vigorous seedling development through to seed production was achieved. Dispersed seeds frequently germinated on top of the foam substrate, yielding a 2nd generation of seedlings. The methods used herein could be applied to other plant species to be flown in space.

  6. Cloud Based Development Issues: A Methodical Analysis

    Directory of Open Access Journals (Sweden)

    Sukhpal Singh

    2012-11-01

    Full Text Available Cloud based development is a challenging task for various software engineering projects, especifically for those which demand extraordinary quality, reusability and security along with general architecture. In this paper we present a report on a methodical analysis of cloud based development problems published in major computer science and software engineering journals and conferences organized by various researchers. Research papers were collected from different scholarly databases using search engines within a particular period of time. A total of 89 research papers were analyzed in this methodical study and we categorized into four classes according to the problems addressed by them. The majority of the research papers focused on quality (24 papers associated with cloud based development and 16 papers focused on analysis and design. By considering the areas focused by existing authors and their gaps, untouched areas of cloud based development can be discovered for future research works.

  7. Recommendation advertising method based on behavior retargeting

    Science.gov (United States)

    Zhao, Yao; YIN, Xin-Chun; CHEN, Zhi-Min

    2011-10-01

    Online advertising has become an important business in e-commerce. Ad recommended algorithms are the most critical part in recommendation systems. We propose a recommendation advertising method based on behavior retargeting which can avoid leakage click of advertising due to objective reasons and can observe the changes of the user's interest in time. Experiments show that our new method can have a significant effect and can be further to apply to online system.

  8. KNOWLEDGE BASED METHODS FOR VIDEO DATA RETRIEVAL

    OpenAIRE

    S.Thanga Ramya; P. Rangarajan

    2011-01-01

    Large collections of publicly available video data grow day by day, the need to query this dataefficiently becomes significant. Consequently, content-based retrieval of video data turns out to be achallenging and important problem. This paper addresses the specific aspect of inferring semanticsautomatically from raw video data using different knowledge-based methods. In particular, this paperfocuses on three techniques namely, rules, Hidden Markov Models (HMMs), and Dynamic BayesianNetworks (...

  9. 基于校企双主体的创意人才培养模式研究%Research on Creative Talents Cultivation Mode Based on Double Subjects of College and Enterprise

    Institute of Scientific and Technical Information of China (English)

    曾文英; 曾东海; 吴积军

    2013-01-01

    Based on the contents of double subjects of college and enterprise, this paper describes the strategy of creative talents to be cultivated, multiple cooperative methods between college and enterprise and talents cultivation mode. The definition of creative talents, combination mode of double subjects between college and enterprise, and the characteristics and paths of creative talents training have been analyzed and discussed. The creative talents training mode based on double subjects of college and enterprise is proposed and the mode can take use of the both resources, with interactive, multiple dimensions expansion, dynamic process, flexible and available, and etc. Some innovative thought and ideas have been proposed, and the category based on cooperation methods have been divided into dynamic state and constant state, the implementation methods have been divided into micro mode, middle mode and macro mode. The development modes contain wide development, deep development and net development.%  通过对校企双主体的内涵为出发点,论述了创意人才培养的策略、校企合作的多种方式构建的人才培养模式。分别对创意人才定义、校企双主体结合模式、以及基于模式的创意人才培养的特点与途径等进行了分析和探讨。提出的基于校企双主体的创意人才培养模式具有综合利用校企资源、全面互动、多维拓展、动态实施、灵活可行等的特点。对于创意人才培养模式提出了一些创新思路和创新理念,并对模式中的协作方式划分为动态和常态,将模式的实现方式分为微模式、中模式、宏模式,将拓展方式归结为横向拓展、纵向拓展和网状拓展。

  10. Spatial distribution of prime farmland based on cultivated land quality comprehensive evaluation at county scale%基于耕地质量综合评价的县域基本农田空间布局

    Institute of Scientific and Technical Information of China (English)

    奉婷; 张凤荣; 李灿; 曲衍波; 朱凤凯

    2014-01-01

    For the cultivated land quality comprehensive evaluation there have been relatively abundant research results at present, but most of them tend to judge the suitability of spatial distribution of cultivated land and prime farmland without taking sufficient attention to the ecological security. The cultivated land quality comprehensive evaluation plays an important part as prerequisites and groundwork in demarcating of prime farmland and high-standard prime farmland construction. On the basis of dissecting the concept and method of cultivated land quality evaluation, the paper constructed a cultivated quality comprehensive evaluation system by way of selecting relevant indicators from the natural quality, site conditions, spatial pattern and ecological security of cultivated land through referring to agricultural land classification, agricultural land gradation, cultivated land scale operation and its ecological security. The study aimed at meeting the inner demands for demarcating of prime farmland and made up for the deficiency in the present related researches, then achieved quantitative analysis of various indexes and calculation on the cultivated land quality comprehensive index with the help of GIS. On the above basis, the study divided 5 916 cultivated land parcels into 4 different types according to the similarity theory in Pinggu district:1) The first one, named as the prior planning type, was up to 4 325.29 hm2 and accounted for 35.06% of regional total cultivated land area, distributed mainly in the western, southern and southwest plains of Pinggu district, mainly in the town of Donggaocun, followed by the towns of Yukou and Mafang, which showed comprehensive optimum in the natural quality, site conditions, ecological security and spatial pattern of cultivated land as the preferred object to be prime farmland or high-standard prime farmland. This type became the core part of cultivated land protected in Pinggu district. 2) The second one, named as the

  11. Oil monitoring methods based on information theory

    Institute of Scientific and Technical Information of China (English)

    XIA Yan-chun; HUO Hua

    2009-01-01

    To evaluate the Wear condition of machines accurately,oil spectrographic entropy,mutual information and ICA analysis methods based on information theory are presented.A full-scale diagnosis utilizing all channels of spectrographic analysis can be obtained.By measuring the complexity and correlativity,the characteristics of wear condition of machines can be shown clearly.The diagnostic quality is improved.The analysis processes of these monitoring methods are given through the explanation of examples.The availability of these methods is validated and further research fields are demonstrated.

  12. Exploration on Effective Teaching Methods of Ideological and Moral Cultivation Course in Junior High Schools%初中思想品德课有效教学法探究

    Institute of Scientific and Technical Information of China (English)

    秦树芬

    2014-01-01

    初中学生正处于青春期,思想品德课对于学生的德育的养成有着重要作用。提高初中思想品德课的教学有效性就要做到:立足教材,营造师生双方互动的课堂。以学生为中心,营造学生喜欢的课堂。以生活为模板,营造师生共乐的课堂。%Junior high school students are adolescents, so ideo-logical and moral cultivation course plays an important role in the development of their moral cultivation. To improve the effective-ness of ideological and moral cultivation teaching in junior high schools, a classroom with teacher-student interaction should be established based on the textbook;a classroom loved by students should be constructed centered by students;a classroom in which both the teacher and students are happy with life as the back-ground.

  13. Identification of suitable sites for mountain ginseng cultivation using GIS and geo-temperature.

    Science.gov (United States)

    Kang, Hag Mo; Choi, Soo Im; Kim, Hyun

    2016-01-01

    This study was conducted to explore an accurate site identification technique using a geographic information system (GIS) and geo-temperature (gT) for locating suitable sites for growing cultivated mountain ginseng (CMG; Panax ginseng), which is highly sensitive to the environmental conditions in which it grows. The study site was Jinan-gun, South Korea. The spatial resolution for geographic data was set at 10 m × 10 m, and the temperatures for various climatic factors influencing CMG growth were calculated by averaging the 3-year temperatures obtained from the automatic weather stations of the Korea Meteorological Administration. Identification of suitable sites for CMG cultivation was undertaken using both a conventional method and a new method, in which the gT was added as one of the most important factors for crop cultivation. The results yielded by the 2 methods were then compared. When the gT was added as an additional factor (new method), the proportion of suitable sites identified decreased by 0.4 % compared with the conventional method. However, the proportion matching real CMG cultivation sites increased by 3.5 %. Moreover, only 68.2 % corresponded with suitable sites identified using the conventional factors; i.e., 31.8 % were newly detected suitable sites. The accuracy of GIS-based identification of suitable CMG cultivation sites improved by applying the temperature factor (i.e., gT) in addition to the conventionally used factors.

  14. 陶冶教学法在《护理专业英语》教学中的应用%Application of cultivating teaching method in the teaching of "professional English of nursing"

    Institute of Scientific and Technical Information of China (English)

    刘燕南; 段功香

    2013-01-01

    目的:探讨陶冶教学法在教学中的应用及效果.方法:选取2009级涉外护理专业1班和2班护生60名,将1班30名作为观察组,应用陶冶教学法;将2班30名作为对照组,应用传统理论教学法.课程结束后,采用问卷调查和考试两种方法比较两组对教学效果评价和考试成绩.结果:观察组对教学效果评价和考试成绩明显优于对照组(P<0.05).结论:陶冶教学法可提高护生的护理英语实际运用能力、自主学习能力及团队合作能力.%Objective:To explore the application of cultivating teaching method in the teaching of "professional English of nursing" and the teaching effect. Methods:60 selected nursing students of 2 classes enrolled in 2009 were divided into the observation group and the control group ( 30 students in each group ). The cultivating teaching method was adopted in the observation group and the traditional theoretic teaching method was used in the control group. At the end of the course,the questionnaire survey and examination were conducted to compare the teaching effect and examination results of the students between the two groups. Results:The teaching effect and examination results of the students were significantly better in the observation group than the control group ( P < 0. 05 ). Conclusion: The cultivating teaching method can improve the practical ability of nursing students to use English,self -learning ability and teamwork ability.

  15. Embedded system-based incubator cultivation of applied talent%基于嵌入式系统应用型人才培养基地的建设

    Institute of Scientific and Technical Information of China (English)

    张乐芳

    2012-01-01

    Based on the opportunities confronted with embedded industrial development,the problems and defects in embedded system teaching among domestic universities are analysed.Practices in guiding principles,educational objectives,curriculum design,training content and instructional models,embedded system-based incubator cultivation of applied talents in Xi'an Eurasia University as an example,are also covered.The author concludes that with embedded system teaching,students' development and application competence has been greatly enhanced and it has also promoted the discipline development and the building of high-quality teaching faculty.%针对嵌入式产业发展所面临的机遇,简要分析了目前国内高校在嵌入式系统教学中存在的问题和不足。基于我院嵌入式系统应用型人才培养基地的建设,介绍了在实施嵌入式人才培养中,从指导思想、培养目标、课程设置、实训内容、教学模式等方面的做法。实践证明,学生在嵌入式开发及应用能力方面有了明显的提升,同时又推动了学科发展及师资队伍建设。

  16. 通信工程类本科研究型人才培养的思考%Considerations for the Cultivation of Research-based Undergraduates in Communication Engineering

    Institute of Scientific and Technical Information of China (English)

    周宁; 李晓峰; 李玉柏; 刘洪盛; 张敏

    2011-01-01

    In this paper, considerations and practices are described for the cultivation of research-based undergraduate communicationalents in our school. Discussion is given mainly in aspects of establishment of the research- based undergraduate programmes based on the society demands, reform of the teaching methods with support of the advanced academic researchs of the university, promotion of the active studies of the students with the direction of the teachers, improvement of the experimental training system and enhancement of the practical-skill training, and nurturing of scientific research attainments and innovation capabilities with various of scientific projects.%本文介绍了对我院通信类本科研究型人才培养的一些思考与探索。我们主要讨论了基于社会需求制定研究型本科人才培养方案;依托学校的学科与研究优势开展教学改革,积极推进教师引导学生主动探究的研究式学习方法;完善实验教学体系与加强实践技能培养;以及通过各类科技项目培养学生科研素质与创新能力等方面。

  17. Sustainability of current GM crop cultivation : Review of people, planet, profit effects of agricultural production of GM crops, based on the cases of soybean, maize, and cotton

    OpenAIRE

    2011-01-01

    This report adresses the question whether the cultivation of genetically modified (GM) crops abroad for import in the Netherlands, as compared to the cultivation of their conventional (non-GM) counterparts, is in line with Dutch policy and societal aims striving after more sustainable forms of agriculture worldwide and the utilization of the benefits offered by biotechnology in a responsible manner. Three crops were selected as case study objects: sybean, maize and cotton. The sustainability ...

  18. Cultivating Common Ground: Integrating Standards-Based Visual Arts, Math and Literacy in High-Poverty Urban Classrooms

    Science.gov (United States)

    Cunnington, Marisol; Kantrowitz, Andrea; Harnett, Susanne; Hill-Ries, Aline

    2014-01-01

    The "Framing Student Success: Connecting Rigorous Visual Arts, Math and Literacy Learning" experimental demonstration project was designed to develop and test an instructional program integrating high-quality, standards-based instruction in the visual arts, math, and literacy. Developed and implemented by arts-in-education organization…

  19. Facial Beautification Method Based on Age Evolution

    Institute of Scientific and Technical Information of China (English)

    CHEN Yan; DING Shou-hong; HU Gan-le; MA Li-zhuang

    2013-01-01

    This paper proposes a new facial beautification method using facial rejuvenation based on the age evolution. Traditional facial beautification methods only focus on the color of skin and deformation and do the transformation based on an experimental standard of beauty. Our method achieves the beauty effect by making facial image looks younger, which is different from traditional methods and is more reasonable than them. Firstly, we decompose the image into different layers and get a detail layer. Secondly, we get an age-related parameter:the standard deviation of the Gaussian distribution that the detail layer follows, and the support vector machine (SVM) regression is used to fit a function about the age and the standard deviation. Thirdly, we use this function to estimate the age of input image and generate a new detail layer with a new standard deviation which is calculated by decreasing the age. Lastly, we combine the original layers and the new detail layer to get a new face image. Experimental results show that this algo-rithm can make facial image become more beautiful by facial rejuvenation. The proposed method opens up a new way about facial beautification, and there are great potentials for applications.

  20. Searching chromosomal landmarks in Indian lentils through EMA-based Giemsa staining method.

    Science.gov (United States)

    Jha, Timir Baran; Halder, Mihir

    2016-09-01

    Lentil is one of the oldest protein-rich food crop with only one cultivated and six wild species. India is one important cultivator, producer and consumer of lentils and possesses a large number of germplasms. All species of lentil show 2n = 14 chromosomes. The primary objective of the present paper is to search chromosomal landmarks through enzymatic maceration and air drying (EMA)-based Giemsa staining method in five Indian lentil species not reported elsewhere at a time. Additionally, gametic chromosome analysis, tendril formation and seed morphology have been studied to ascertain interspecific relationships in lentils. Chromosome analysis in Lens culinaris, Lens orientalis and Lens odemensis revealed that they contain intercalary sat chromosome and similar karyotypic formula, while Lens nigricans and Lens lamottei showed presence of terminal sat chromosomes not reported earlier. This distinct morphological feature in L. nigricans and L. lamottei may be considered as chromosomal landmark. Meiotic analysis showed n = 7 bivalents in L. culinaris, L. nigricans and L. lamottei. No tendril formation was observed in L. culinaris, L. orientalis and L. odemensis while L. nigricans and L. lamottei developed very prominent tendrils. Based on chromosomal analysis, tendril formation and seed morphology, the five lentil species can be separated into two distinct groups. The outcome of this research may enrich conventional and biotechnological breeding programmes in lentil and may facilitate an easy and alternative method for identification of interspecific hybrids.

  1. Research on the method for removing the influential factor for lymphocyte growth during cultivation%去除影响淋巴细胞培养生长因素的方法探讨

    Institute of Scientific and Technical Information of China (English)

    聂俊玮; 梁燕; 杨寿; 唐玉芬

    2008-01-01

    目的 探讨影响淋巴细胞培养生长因素及去除方法.方法 在正常淋巴细胞培养中去掉原培养液,加入灭菌生理水(内含有青霉素钠100 IU/mL)洗涤后再加入培养基继续培养,常规收获细胞及常规G显带油镜观察分析.结果 用上述方法洗涤后收获的细胞较正常培养收获的细胞转化率、有丝分裂指数、分裂相质量均提高非常显著(P<0.01).结论 该方法可去除影响淋巴细胞培养生长的因素,保证收获细胞数量和质量,从而提高分析诊断水平,且易于推广应用.%Objective To probe into the influential factor for lymphocyte growth and its removing method.Methods The original culture liquid in normal lymphocyte medium was removed and washed with the disinfectant physiological saline(containing 100 IU/mL of benzylpenicillin sodium).After that,the new culture medium was added for continuous cultivation.The lymphocytes had been collected conventionally and G banding analysis under immersion objective was performed.Results After the aforesaid washing method was used,cell transformation efficiency,mitotic index and quality of mitotic phase of lymphocytes were obviously improved compared with those cultivated by conventional cultivation method(P<0.01).Conclusion It is proved that this new method can remove the influence on lymphocyte growth during cultivation and guarantee the harvesting cell number and quality,and then improve the levels of analysis and diagnosis.And also it is easy for popularization.

  2. 虚拟情境教学法在专业英语人才培养中的应用研究%Virtual Situational Teaching Method in the Application of Professional English Talent Cultivation Research

    Institute of Scientific and Technical Information of China (English)

    王梅亮; 叶秋群

    2014-01-01

    为了适应新的经济时代对应用型英语专业人才的需求,迫切需要改革目前专业英语的教学模式。该文首先根据建构主义教学理论,探讨虚拟情境教学法在专业英语教学中应用的优越性;然后研究如何在专业英语课堂教学中利用虚拟情境教学系统,为学生营造出逼真的语言学习和运用环境,通过情景体验进行教学和操练来提高学英语的学习效果,为培养应用型英语专业人才探索出一种全新而且有效的教学模式和教学手段。%In order to adapt to the new economic era demand for applied English professional talents, the current professional English teaching mode need to be reformed urgently.The paper based on the constructivism teaching theory, discusses the applica-tion of virtual situational teaching method in the professional English teaching superiority. And then it studied how to use virtual situation teaching in English classroom teaching system, in order to create a vivid language learning and using environment for students. Through the scene experience and practice of teaching to improve the learning effect of learning English, we can culti-vate applied English professional talents and explore a new effective teaching mode and teaching means.

  3. 创新高校学生干部队伍培养之路%Innovation of the Cultivation Methods for College Student Cadre Group

    Institute of Scientific and Technical Information of China (English)

    方君亮; 宣玉梅; 成晓

    2011-01-01

    高校学生干部是大学校园中的一个特殊群体,也是高校学生工作的一个重要方面。如何打造一支高素质的学生干部队伍,对于做好高校的思想政治工作和学生工作都具有重要意义。本文着重研究如何培养学生干部自身素质、如何培养学生干部团队合作意识和如何在活动中提升学生干部队伍综合素质。%Student cadre is a special group on college campus and an important aspect of college student affairs.How to construct a high quality student cadre group is of great significance to implement college ideological and political work and student affairs.This paper mainly studies on how to cultivate the quality of student cadres,how to cultivate the teamwork awareness of student cadre group and how to improve the comprehensive quality of student cadre group.

  4. iTRAQ-based quantitative proteomic analysis reveals proteomic changes in leaves of cultivated tobacco (Nicotiana tabacum) in response to drought stress.

    Science.gov (United States)

    Xie, He; Yang, Da-Hai; Yao, Heng; Bai, Ge; Zhang, Yi-Han; Xiao, Bing-Guang

    2016-01-15

    Drought is one of the most severe forms of abiotic stresses that threaten the survival of plants, including crops. In turn, plants dramatically change their physiology to increase drought tolerance, including reconfiguration of proteomes. Here, we studied drought-induced proteomic changes in leaves of cultivated tobacco (Nicotiana tabacum), a solanaceous plant, using the isobaric tags for relative and absolute quantitation (iTRAQ)-based protein labeling technology. Of identified 5570 proteins totally, drought treatment increased and decreased abundance of 260 and 206 proteins, respectively, compared with control condition. Most of these differentially regulated proteins are involved in photosynthesis, metabolism, and stress and defense. Although abscisic acid (ABA) levels greatly increased in drought-treated tobacco leaves, abundance of detected ABA biosynthetic enzymes showed no obvious changes. In contrast, heat shock proteins (HSPs), thioredoxins, ascorbate-, glutathione-, and hydrogen peroxide (H2O2)-related proteins were up- or down-regulated in drought-treated tobacco leaves, suggesting that chaperones and redox signaling are important for tobacco tolerance to drought, and it is likely that redox-induced posttranslational modifications play an important role in modulating protein activity. This study not only provides a comprehensive dataset on overall protein changes in drought-treated tobacco leaves, but also shed light on the mechanism by which solanaceous plants adapt to drought stress.

  5. New insights into the biogeochemistry of extremely acidic environments revealed by a combined cultivation-based and culture-independent study of two stratified pit lakes.

    Science.gov (United States)

    Falagán, Carmen; Sánchez-España, Javier; Johnson, David Barrie

    2014-01-01

    The indigenous microbial communities of two extremely acidic, metal-rich stratified pit lakes, located in the Iberian Pyrite Belt (Spain), were identified, and their roles in mediating transformations of carbon, iron, and sulfur were confirmed. A combined cultivation-based and culture-independent approach was used to elucidate microbial communities at different depths and to examine the physiologies of isolates, which included representatives of at least one novel genus and several species of acidophilic Bacteria. Phosphate availability correlated with redox transformations of iron, and this (rather than solar radiation) dictated where primary production was concentrated. Carbon fixed and released as organic compounds by acidophilic phototrophs acted as electron donors for acidophilic heterotrophic prokaryotes, many of which catalyzed the dissimilatory reduction in ferric iron; the ferrous iron generated was re-oxidized by chemolithotrophic acidophiles. Bacteria that catalyze redox transformations of sulfur were also identified, although these Bacteria appeared to be less abundant than the iron oxidizers/reducers. Primary production and microbial numbers were greatest, and biogeochemical transformation of carbon, iron, and sulfur, most intense, within a zone of c. 8-10 m depth, close to the chemocline, in both pit lakes. Archaea detected in sediments included two Thaumarchaeota clones, indicating that members of this recently described phylum can inhabit extremely acidic environments.

  6. Cultivation of native seaweed Gracilaria domingensis (Rhodophyta in Southern Brazil

    Directory of Open Access Journals (Sweden)

    José Pedrassoli Salles

    2010-06-01

    Full Text Available The aim of this work was to study the cultivation of Gracilaria domingensis in a mussel farming urbanized area in Santa Catarina, Brazil. Relative growth rate was the parameter used to evaluate the cuttings attachment methods on the cultivation rope, cuttings density, cultivation period and cystocarpic versus unfertile thalli performance. The cultivation was feasible only when protected by net cages due to herbivory. The tie-tie attachment method presented the best results. No differences were observed when comparing the cuttings densities and reproductive phase. Future studies should evaluate the cost-effectiveness of producing the species in net cages and its potential as biofilter.

  7. AN SVAD ALGORITHM BASED ON FNNKD METHOD

    Institute of Scientific and Technical Information of China (English)

    Chen Dong; Zhang Yan; Kuang Jingming

    2002-01-01

    The capacity of mobile communication system is improved by using Voice Activity Detection (VAD) technology. In this letter, a novel VAD algorithm, SVAD algorithm based on Fuzzy Neural Network Knowledge Discovery (FNNKD) method is proposed. The performance of SVAD algorithm is discussed and compared with traditional algorithm recommended by ITU G.729B in different situations. The simulation results show that the SVAD algorithm performs better.

  8. Need to reform education in graduate schools of public health in Japan: Toward outcome-based education to cultivate competencies.

    Science.gov (United States)

    Inoue, Mariko; Yano, Eiji

    2016-01-01

    Japan, known for its good healthcare access via universal health insurance, leads the world in terms of life expectancy, and possesses a public health system that has improved health standards markedly in the 20th century. However, we currently face major challenges to maintain and promote people's health. Although these complicated problems pose numerous threats to public welfare, education of public health for health professionals still retains 20th-century standards. This also means that graduate education of public health in Japan is traditionally based on obtaining licensure as a medical professional, conducting research and writing papers, and on-the-job training. Since graduate school education is expected to produce competent public health leaders, Japan requires a reform toward a new education design that caters to the current societal needs. The current global trend in the education of health professionals leans toward outcome-based education to meet core competencies. Here, "competency" refers to a set of features or particular behavioral patterns possessed by highly qualified persons. In 2006, the World Health Organization (WHO) established a general health professional competency standard that includes both management and leadership competencies. Moreover, the Lancet Commission concluded that there was a need for transformative education based on a "health system approach." In brief, this means that our education should correspond to the needs of the health system to allow for the resolution of problems by educated professionals with satisfactory levels of competencies. In addition, as "change agents," these competent professionals are expected to promote societal change toward the realization of better public health. In Japan, the Central Education Council has produced several reports on professional graduate school reform since 2000. These reports indicate that graduate school curricula require reform to allow the health professionals to work

  9. Life cycle assessment of hemp cultivation and use of hemp-based thermal insulator materials in buildings.

    Science.gov (United States)

    Zampori, Luca; Dotelli, Giovanni; Vernelli, Valeria

    2013-07-02

    The aim of this research is to assess the sustainability of a natural fiber, such as hemp (Cannabis sativa), and its use as thermal insulator for building applications. The sustainability of hemp was quantified by life cycle assessment (LCA) and particular attention was given to the amount of CO2eq of the whole process, and the indicator greenhouse gas protocol (GGP) was selected to quantify CO2eq emissions. In this study also CO2 uptake of hemp was considered. Two different allocation procedures (i.e., mass and economic) were adopted. Other indicators, such as Cumulative Energy Demand (CED) and EcoIndicator99 H were calculated. The production of 1 ha yielded 15 ton of hemp, whose global warming potential (GWP100) was equal to about -26.01 ton CO2eq: the amount allocated to the technical fiber (20% of the total amount of hemp biomass) was -5.52 ton CO2eq when mass allocation was used, and -5.54 ton CO2eq when economic allocation was applied. The sustainability for building applications was quantified by considering an insulation panel made by hemp fiber (85%) and polyester fiber (15%) in 1 m(2) of wall having a thermal transmittance (U) equal to 0.2 W/m(2)_K. The environmental performances of the hemp-based panel were compared to those of a rockwool-based one.

  10. Cultivation of evidence-based quality of clinical specialization postgraduates%临床医学专业学位硕士研究生循证素质培养

    Institute of Scientific and Technical Information of China (English)

    曲彦隆; 薄红; 关策文

    2011-01-01

    在临床医学专业学位硕士研究生教育中,循证素质培养是十分必要的.哈尔滨医科大学第一附属医院结合PBL教学,从循证意识的培养、循证能力的培养、建立评价体系三个方面,进行临床医学专业学位硕士研究生循证素质培养.%The importance of cultivating evidence-based consciousness of thinking and implementation capability of specialization postgraduates was analyzed,and experiences and suggestions acquired from the practice of teaching of specialization postgraduates were shared by demonstrating the enlightening of evidence-based consciousness of thinking,the cultivation of evidence-based implementation capability and the establishment of evaluation system,with PBL integrated.

  11. Lagrangian based methods for coherent structure detection

    Energy Technology Data Exchange (ETDEWEB)

    Allshouse, Michael R., E-mail: mallshouse@chaos.utexas.edu [Center for Nonlinear Dynamics and Department of Physics, University of Texas at Austin, Austin, Texas 78712 (United States); Peacock, Thomas, E-mail: tomp@mit.edu [Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 (United States)

    2015-09-15

    There has been a proliferation in the development of Lagrangian analytical methods for detecting coherent structures in fluid flow transport, yielding a variety of qualitatively different approaches. We present a review of four approaches and demonstrate the utility of these methods via their application to the same sample analytic model, the canonical double-gyre flow, highlighting the pros and cons of each approach. Two of the methods, the geometric and probabilistic approaches, are well established and require velocity field data over the time interval of interest to identify particularly important material lines and surfaces, and influential regions, respectively. The other two approaches, implementing tools from cluster and braid theory, seek coherent structures based on limited trajectory data, attempting to partition the flow transport into distinct regions. All four of these approaches share the common trait that they are objective methods, meaning that their results do not depend on the frame of reference used. For each method, we also present a number of example applications ranging from blood flow and chemical reactions to ocean and atmospheric flows.

  12. An attribute-based image segmentation method

    Directory of Open Access Journals (Sweden)

    M.C. de Andrade

    1999-07-01

    Full Text Available This work addresses a new image segmentation method founded on Digital Topology and Mathematical Morphology grounds. The ABA (attribute based absorptions transform can be viewed as a region-growing method by flooding simulation working at the scale of the main structures of the image. In this method, the gray level image is treated as a relief flooded from all its local minima, which are progressively detected and merged as the flooding takes place. Each local minimum is exclusively associated to one catchment basin (CB. The CBs merging process is guided by their geometric parameters as depth, area and/or volume. This solution enables the direct segmentation of the original image without the need of a preprocessing step or the explicit marker extraction step, often required by other flooding simulation methods. Some examples of image segmentation, employing the ABA transform, are illustrated for uranium oxide samples. It is shown that the ABA transform presents very good segmentation results even in presence of noisy images. Moreover, it's use is often easier and faster when compared to similar image segmentation methods.

  13. 考虑耕地生态质量的基本农田划定方法%Demarcating method of prime farmland considering ecological quality of cultivated land

    Institute of Scientific and Technical Information of China (English)

    任艳敏; 唐秀美; 刘玉; 潘瑜春

    2014-01-01

    Evaluating the quality of cultivated land comprehensively and systematically is an important prerequisite in performing, demarcating, and conducting high standards of prime farmland scientifically. Taking Tunchang county, Hainan Province as an example, these indexes are chosen to evaluate the quality of cultivated land in the paper as following: topography, erosion condition of soil, ecological system service value, flood disaster, connection, etc, on the basis of comprehensive consideration of nature quality and location condition. It shows that the ecological quality is very important in prime farmland evaluation, and index system is improved. The result: 88.91% of cultivated land is demarcated into prime farmland, with comprehensive evaluating score equal or larger than 70, mainly in the central south and north of the platforms and plains regions of Tunchang country. Contrastive analysis was studied between the method of this paper (method 1) and natural quality of agricultural land (method 2) in demarcating prime farmland. Cultivated land of the study area is classified into four type regions, namely: Type region I, Type region II, Type region III, Type region IV. Among them, Type region I is described as the farmland area demarcated by the above two methods, which is the largest part (84.49%) and has some essential characteristics such as good nature quality, convenient location convenient, less natural disasters, and soil erosion, as well as fairly flat land. Except that, prime farmland is close to towns or rural settlements in spatial distribution, and their average patch area is large and is in the obviously centralized distribution, which is easy to agricultural scaled production and mechanization. Type region II is chosen through method 2, only occupies 4.42%of the area, but distributes in every town. This kind of cultivated land is mainly west and east of the mountainous and hilly regions. The patches are much smaller than Type region I or Type region

  14. Chapter 11. Community analysis-based methods

    Energy Technology Data Exchange (ETDEWEB)

    Cao, Y.; Wu, C.H.; Andersen, G.L.; Holden, P.A.

    2010-05-01

    Microbial communities are each a composite of populations whose presence and relative abundance in water or other environmental samples are a direct manifestation of environmental conditions, including the introduction of microbe-rich fecal material and factors promoting persistence of the microbes therein. As shown by culture-independent methods, different animal-host fecal microbial communities appear distinctive, suggesting that their community profiles can be used to differentiate fecal samples and to potentially reveal the presence of host fecal material in environmental waters. Cross-comparisons of microbial communities from different hosts also reveal relative abundances of genetic groups that can be used to distinguish sources. In increasing order of their information richness, several community analysis methods hold promise for MST applications: phospholipid fatty acid (PLFA) analysis, denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (TRFLP), cloning/sequencing, and PhyloChip. Specific case studies involving TRFLP and PhyloChip approaches demonstrate the ability of community-based analyses of contaminated waters to confirm a diagnosis of water quality based on host-specific marker(s). The success of community-based MST for comprehensively confirming fecal sources relies extensively upon using appropriate multivariate statistical approaches. While community-based MST is still under evaluation and development as a primary diagnostic tool, results presented herein demonstrate its promise. Coupled with its inherently comprehensive ability to capture an unprecedented amount of microbiological data that is relevant to water quality, the tools for microbial community analysis are increasingly accessible, and community-based approaches have unparalleled potential for translation into rapid, perhaps real-time, monitoring platforms.

  15. Evidence for a Common Origin of Blacksmiths and Cultivators in the Ethiopian Ari within the Last 4500 Years: Lessons for Clustering-Based Inference.

    Directory of Open Access Journals (Sweden)

    Lucy van Dorp

    2015-08-01

    Full Text Available The Ari peoples of Ethiopia are comprised of different occupational groups that can be distinguished genetically, with Ari Cultivators and the socially marginalised Ari Blacksmiths recently shown to have a similar level of genetic differentiation between them (FST ≈ 0.023 - 0.04 as that observed among multiple ethnic groups sampled throughout Ethiopia. Anthropologists have proposed two competing theories to explain the origins of the Ari Blacksmiths as (i remnants of a population that inhabited Ethiopia prior to the arrival of agriculturists (e.g. Cultivators, or (ii relatively recently related to the Cultivators but presently marginalized in the community due to their trade. Two recent studies by different groups analysed genome-wide DNA from samples of Ari Blacksmiths and Cultivators and suggested that genetic patterns between the two groups were more consistent with model (i and subsequent assimilation of the indigenous peoples into the expanding agriculturalist community. We analysed the same samples using approaches designed to attenuate signals of genetic differentiation that are attributable to allelic drift within a population. By doing so, we provide evidence that the genetic differences between Ari Blacksmiths and Cultivators can be entirely explained by bottleneck effects consistent with hypothesis (ii. This finding serves as both a cautionary tale about interpreting results from unsupervised clustering algorithms, and suggests that social constructions are contributing directly to genetic differentiation over a relatively short time period among previously genetically similar groups.

  16. Custom Fusion Methode Based on Borda

    Directory of Open Access Journals (Sweden)

    Issam Abdelbaki

    2016-10-01

    Full Text Available Searching for information on the Internet is not only an activity newly rediscovered, but also a strategic tool to achieve a wide variety of information. Indeed, it’s extremely important to know how to find the information quickly and efficiently. Unfortunately, the Web is so huge and so little structured, that gathering precise, fair and useful information becomes an expensive task. In order to define an information retrieval tool (meta search engine that brings together multiple sources of information search, interest must be credited to the merger phase of search engines results. On the other hand, information search systems tend primarily to model the user with a profile and then to integrate it into the information access chain, to better meet its specific needs. This paper presents a custom fusion method based on Borda method and values retrieved from the user profile. We evaluated our approach on multiple domains and we present some experimental results.

  17. Kernel method-based fuzzy clustering algorithm

    Institute of Scientific and Technical Information of China (English)

    Wu Zhongdong; Gao Xinbo; Xie Weixin; Yu Jianping

    2005-01-01

    The fuzzy C-means clustering algorithm(FCM) to the fuzzy kernel C-means clustering algorithm(FKCM) to effectively perform cluster analysis on the diversiform structures are extended, such as non-hyperspherical data, data with noise, data with mixture of heterogeneous cluster prototypes, asymmetric data, etc. Based on the Mercer kernel, FKCM clustering algorithm is derived from FCM algorithm united with kernel method. The results of experiments with the synthetic and real data show that the FKCM clustering algorithm is universality and can effectively unsupervised analyze datasets with variform structures in contrast to FCM algorithm. It is can be imagined that kernel-based clustering algorithm is one of important research direction of fuzzy clustering analysis.

  18. Trinocular Calibration Method Based on Binocular Calibration

    Directory of Open Access Journals (Sweden)

    CAO Dan-Dan

    2012-10-01

    Full Text Available In order to solve the self-occlusion problem in plane-based multi-camera calibration system and expand the measurement range, a tri-camera vision system based on binocular calibration is proposed. The three cameras are grouped into two pairs, while the public camera is taken as the reference to build the global coordinate. By calibration of the measured absolute distance and the true absolute distance, global calibration is realized. The MRE (mean relative error of the global calibration of the two camera pairs in the experiments can be as low as 0.277% and 0.328% respectively. Experiment results show that this method is feasible, simple and effective, and has high precision.

  19. 基于Unity3D的甘蔗种植虚拟教育培训系统设计与实现%Design and Realization of Virtual Education Training System for Sugarcane Cultivation Based on Unity3D

    Institute of Scientific and Technical Information of China (English)

    吴升; 郭新宇; 贺谊; 温维亮; 王虓

    2014-01-01

    我国是最大的甘蔗种植国之一,甘蔗种植教育培训课程在各大农林职业院校广泛开展,针对传统的甘蔗种植教育培训试验开展周期长、成本高、授课方式枯燥、参与实践机会少等不足。为此开展甘蔗生长模型建模,农事仿真实时可视化等虚拟仿真交互关键技术进行研究;以“看动画、学知识、玩游戏、长技能”的虚拟互动培训理念,提出基于时序驱动的甘蔗虚拟种植教育培训系统设计方法;以Unity3D为开发平台,实现甘蔗种植生长周期虚拟展示以及互动体验功能。系统以虚拟化改造传统教学,为现代农业职业技术教育提供新的教学思路,从而为全面提升学员技术技能水平做出贡献。%China is one of the largest sugarcane cultivation countries. Education training courses for sugarcane cultivation is conducted in vocational colleges of agriculture and forestry universities. On account of the shortcomings of the conventional sugarcane education and training, such as long cycles, high costs, boring teaching methods, few practice opportunities, etc., we carried out studies on key technology of sugarcane interactive virtual simulation, sugarcane growth modeling, sugarcane planting real⁃time visualization simulation, etc. Method for designing sugarcane virtual education training system based on timing driven was provided, taking the concept of “watching animation, learning knowledge, playing games, and mastering skill” throughout the whole teaching process. Thus, taking Unity3D as a development platform, we realized the virtual display of sugarcane growth cycle and interactive experience function. This system took virtualization to reform the traditional education, and provided new teaching ideas for modern vocational and technical education in agriculture, thus had comprehensive upgrading of the students’ technical skill level.

  20. On Task-based English Learning Method

    Institute of Scientific and Technical Information of China (English)

    朱蕾

    2010-01-01

    @@ Task-Based learning(TBL)is becoming a catchword in English circles.The new national English Curricular Syllabus also recommends the use of the TBL approach in classroom teaching.The purpose of learning a foreign language is the most direct communicative in the target language,and speaking is the most direct communicative method.In recent years,with the publication of the New Curriculum Standard by the State Education Department,the teaching reform in middle and primary schools has been being implemented step by step.

  1. 基于能力培养和团队协作的通信电子电路实验考核模式%Assessment mode for communication electronic circuit experiment based on capacity cultivation and team cooperation

    Institute of Scientific and Technical Information of China (English)

    车辚辚; 余萍; 程文清; 李然

    2012-01-01

    针对通信电子电路实验教学改革,提出了基于能力培养和团队协作的通信电子电路实验考核模式.该考核模式以表现性评价为标准,对学生的实验成绩进行量化评价,着重对学生的综合实验技能和团队协作能力进行考核.新的考核模式有助于提高教学质量和学生素质,并且考核结果具有更强的可靠性和公平性.%In view of teaching reform of communication electronic circuit experiment, the assessment mode for communication electronic circuit experiment based on ccapacity cultivation and team cooperation has been proposed, which takes the performance assessment as the standard. Experimental scores of students can be got by the mode with the quantifiable assessment. The assessment mode focuses on students' comprehensive experiment quality and team cooperation. Practice shows the effectiveness of proposed method in teaching quality and improving students' quality. Moreover assessment results are greater reliability and fairness.

  2. Bus Based Synchronization Method for CHIPPER Based NoC

    Directory of Open Access Journals (Sweden)

    D. Muralidharan

    2016-01-01

    Full Text Available Network on Chip (NoC reduces the communication delay of System on Chip (SoC. The main limitation of NoC is power consumption and area overhead. Bufferless NoC reduces the area complexity and power consumption by eliminating buffers in the traditional routers. The bufferless NoC design should include live lock freeness since they use hot potato routing. This increases the complexity of bufferless NoC design. Among the available propositions to reduce this complexity, CHIPPER based bufferless NoC is considered as one of the best options. Live lock freeness is provided in CHIPPER through golden epoch and golden packet. All routers follow some synchronization method to identify a golden packet. Clock based method is intuitively followed for synchronization in CHIPPER based NoCs. It is shown in this work that the worst-case latency of packets is unbearably high when the above synchronization is followed. To alleviate this problem, broadcast bus NoC (BBus NoC approach is proposed in this work. The proposed method decreases the worst-case latency of packets by increasing the golden epoch rate of CHIPPER.

  3. Building a field- and model-based climatology of local water and energy cycles in the cultivated Sahel - annual budgets and seasonality

    Science.gov (United States)

    Velluet, C.; Demarty, J.; Cappelaere, B.; Braud, I.; Issoufou, H. B.-A.; Boulain, N.; Ramier, D.; Mainassara, I.; Charvet, G.; Boucher, M.; Chazarin, J.-P.; Oï, M.; Yahou, H.; Maidaji, B.; Arpin-Pont, F.; Benarrosh, N.; Mahamane, A.; Nazoumou, Y.; Favreau, G.; Seghieri, J.

    2014-05-01

    In the African Sahel, energy and water cycling at the land surface is pivotal for regional climate, water resources and land productivity, yet it is still extremely poorly documented. As a step towards a comprehensive climatological description of surface fluxes in this area, this study provides estimates of average annual budgets and seasonal cycles for two main land use types of the cultivated Sahelian belt, rainfed millet crop and fallow bush. These estimates build on the combination of a 7 year field dataset from two typical plots in southwestern Niger with detailed physically-based soil-plant-atmosphere modelling, yielding a continuous, comprehensive set of water and energy flux and storage variables over the 7 year period. In this study case in particular, blending field data with mechanistic modelling is considered as making best use of available data and knowledge for such purpose. It extends observations by reconstructing missing data and extrapolating to unobserved variables or periods. Furthermore, model constraining with observations compromises between extraction of observational information content and integration of process understanding, hence accounting for data imprecision and departure from physical laws. Climatological averages of all water and energy variables, with associated sampling uncertainty, are derived at annual to subseasonal scales from the 7 year series produced. Similarities and differences in the two ecosystems behaviors are highlighted. Mean annual evapotranspiration is found to represent ~82-85% of rainfall for both systems, but with different soil evaporation/plant transpiration partitioning and different seasonal distribution. The remainder consists entirely of runoff for the fallow, whereas drainage and runoff stand in a 40-60% proportion for the millet field. These results should provide a robust reference for the surface energy- and water-related studies needed in this region. The model developed in this context has the

  4. Building a field- and model-based climatology of local water and energy cycles in the cultivated Sahel – annual budgets and seasonality

    Directory of Open Access Journals (Sweden)

    C. Velluet

    2014-05-01

    Full Text Available In the African Sahel, energy and water cycling at the land surface is pivotal for regional climate, water resources and land productivity, yet it is still extremely poorly documented. As a step towards a comprehensive climatological description of surface fluxes in this area, this study provides estimates of average annual budgets and seasonal cycles for two main land use types of the cultivated Sahelian belt, rainfed millet crop and fallow bush. These estimates build on the combination of a 7 year field dataset from two typical plots in southwestern Niger with detailed physically-based soil-plant-atmosphere modelling, yielding a continuous, comprehensive set of water and energy flux and storage variables over the 7 year period. In this study case in particular, blending field data with mechanistic modelling is considered as making best use of available data and knowledge for such purpose. It extends observations by reconstructing missing data and extrapolating to unobserved variables or periods. Furthermore, model constraining with observations compromises between extraction of observational information content and integration of process understanding, hence accounting for data imprecision and departure from physical laws. Climatological averages of all water and energy variables, with associated sampling uncertainty, are derived at annual to subseasonal scales from the 7 year series produced. Similarities and differences in the two ecosystems behaviors are highlighted. Mean annual evapotranspiration is found to represent ~82–85% of rainfall for both systems, but with different soil evaporation/plant transpiration partitioning and different seasonal distribution. The remainder consists entirely of runoff for the fallow, whereas drainage and runoff stand in a 40–60% proportion for the millet field. These results should provide a robust reference for the surface energy- and water-related studies needed in this region. The model developed in

  5. High-throughput micro-scale cultivations and chromatography modeling: Powerful tools for integrated process development.

    Science.gov (United States)

    Baumann, Pascal; Hahn, Tobias; Hubbuch, Jürgen

    2015-10-01

    Upstream processes are rather complex to design and the productivity of cells under suitable cultivation conditions is hard to predict. The method of choice for examining the design space is to execute high-throughput cultivation screenings in micro-scale format. Various predictive in silico models have been developed for many downstream processes, leading to a reduction of time and material costs. This paper presents a combined optimization approach based on high-throughput micro-scale cultivation experiments and chromatography modeling. The overall optimized system must not necessarily be the one with highest product titers, but the one resulting in an overall superior process performance in up- and downstream. The methodology is presented in a case study for the Cherry-tagged enzyme Glutathione-S-Transferase from Escherichia coli SE1. The Cherry-Tag™ (Delphi Genetics, Belgium) which can be fused to any target protein allows for direct product analytics by simple VIS absorption measurements. High-throughput cultivations were carried out in a 48-well format in a BioLector micro-scale cultivation system (m2p-Labs, Germany). The downstream process optimization for a set of randomly picked upstream conditions producing high yields was performed in silico using a chromatography modeling software developed in-house (ChromX). The suggested in silico-optimized operational modes for product capturing were validated subsequently. The overall best system was chosen based on a combination of excellent up- and downstream performance.

  6. Black truffle cultivation: a global reality

    Directory of Open Access Journals (Sweden)

    Santiago Reyna

    2014-08-01

    Full Text Available Aim of study: In recent decades the cultivation of the black truffle Tuber melanosporum has expanded across all the Mediterranean-climate regions, but also to other regions outside the European standard for the species. We aim to describe the current extent of T. melanosporum cultivation.Area of study: Tuber melanosporum plantations in Europe, the Mediterranean basin, Australia, New Zealand, China, America and South Africa.Material and Methods: The socioeconomic impact of T. melanosporum cultivation, the way in which the current situation has been achieved and the knowledge needed for its progress are reviewed.Research highlights: T. melanosporum has been successfully cultivated in several countries outside its natural area, but many practices are still empirical and thus yields cannot be guaranteed. The recent advances in molecular techniques and genome science are helping to overcome some of the difficulties traditionally constraining truffle research. The role of truffles as a transitional element between agricultural and forestry activities makes its cultivation a paradigm of sustainable rural development.Keywords: Tuber melanosporum; Europe; Australia; New Zealand; Chile; USA.

  7. Graph-based Methods for Orbit Classification

    Energy Technology Data Exchange (ETDEWEB)

    Bagherjeiran, A; Kamath, C

    2005-09-29

    An important step in the quest for low-cost fusion power is the ability to perform and analyze experiments in prototype fusion reactors. One of the tasks in the analysis of experimental data is the classification of orbits in Poincare plots. These plots are generated by the particles in a fusion reactor as they move within the toroidal device. In this paper, we describe the use of graph-based methods to extract features from orbits. These features are then used to classify the orbits into several categories. Our results show that existing machine learning algorithms are successful in classifying orbits with few points, a situation which can arise in data from experiments.

  8. Measurements of chlorophyll fluorescence as an auxiliary method in estimating susceptibility of cultivated hazel (Corylus L. for filbert aphid (Myzocallis coryli goetze

    Directory of Open Access Journals (Sweden)

    Magdalena Gantner

    2012-12-01

    Full Text Available The influence of aphid feeding on chlorophyll a fluorescence in the leaves of four cultivated hazel cultivars, with different levels of resistance to filbert aphid (Myzocallis coryli Goetze, was studied. The maximum effect of photosystem reaction measured on dark-adapted hazel leaves (Fv/Fm parameter and maximum efficiency of photon energy PAR conversion to chemical energy in light conditions (Y parameter were estimated twice, in the leaves of four hazel cultivars with different levels of resistance to filbert aphid, using a fluorometer PAM- 2000 by Walz GmbH - Germany. The analysis of changes of these parameters showed that aphid feeding caused a reaction in all tested cultivars. The most visible reduction of the Fv/Fm and Y values as a result of aphid feeding was observed in the cultivars 'Cud z Bollwiller' and 'Olbrzymi z Halle', numerously colonized by aphids. A smaller number of aphids found on the leaves of more resistant cultivars - 'Kataloński' and 'Lamberta Biały', caused a weaker response of plants and a smaller decline in the value of this parameter. 'Cud z Bollwiller' cultivar showed higher tolerance than other tested cultivars to stress caused by the feeding of sucking insects. The Fv/Fm and Y parameters can be regarded as reliable indexes useful in diagnosing susceptibility of hazel cultivars to aphids, helpful in determining, for example, harmfulness thresholds.

  9. 体育教学中培养学生自信心的方法%Methods of Cultivating students’ Confidence in Physical Education Teaching

    Institute of Scientific and Technical Information of China (English)

    聂鹏

    2014-01-01

    体育作为初中生学习的必备课程之一,有助于丰富学生学习生活,增添学生学习乐趣,提高学生的自信心。新课程改革也提出了体育作为全民运动的活动项目,有利于保持人们的身心健康。而初中生作为祖国未来的接班人,并且正处于身体发育和心理成熟的关键时期,体育运动对培养学生的自信心尤为关键。%The sports as a junior high school one of the essential courses of study,help to enrich the students’ life,increase students’ interest in learning,improve students’ confidence. The new curriculum reform put forward,sports as a national sports event,is conducive to maintaining the physical and mental health of people. And junior high school students as the successors,and are in the physical and psychological maturity of the critical period,sports is especially important in the cultivation of students’ self-confidence ability.

  10. Method of Cultivating Students’ Interest in Mathematics Teaching in Primary School%小学数学教学中培养学生兴趣的方法

    Institute of Scientific and Technical Information of China (English)

    郭小军

    2014-01-01

    爱因斯坦说:“兴趣是最好的老师。”可见兴趣对一个人的学习起着至关重要的作用。对于小学生来说,小学数学是一门枯燥而又令人头疼的课程,他们不仅要面对那无趣乏味的课本,还要面对紧张的课堂气氛。如何解决这一问题呢?这就需要教师在小学数学教学中努力培养学生学习数学的兴趣。%Einstein said:“interest is the best teacher”.Visible on a person’s learning interest plays a vital role in. For primary school stu-dents,primary school mathematics is a dull and headaches, they not only have to face the dull textbooks, but also face the tense atmosphere in the classroom. How to solve theproblems in mathematics teaching .This requires teachers to work in primary school mathematics teaching to cultivate students' interest in mathematics.

  11. An Evaluation of the Externality of Cultivated Land Based on CVM:A Case Study of Neihuang County, Henan Province%基于CVM耕地资源利用的外部性评估——以河南省内黄县为例

    Institute of Scientific and Technical Information of China (English)

    陈艳蕊; 赵凯; 王志晓; 薛欧

    2011-01-01

    improve farmland landscape. This helps enhance farmer's awareness to protect cultivated land so as to improve the overall efficiency of land utilization. Based on questionnaire surveys about Neihuang County in Henan Province, we performed Pearson correlation tests about the socio-economic characteristics, and subsequently multiple linear regression analysis on the dependent variables of LnWTP. In addition, we assessed the Externality of the Cultivated Land with Contingent Valuation Method (CVM) and analyzed its influential factors. Results show that: 1) the relationship between annual income of urban households and their willingness to pay is statistically significantly positive; 2) the relationship between the age of rural residents and the corresponding area of contracted cultivated land and their willingness to pay is statistically significantly negative; the relationship among gender, education level, household annual income, agricultural income, and their willingness to pay are generally positive; and 3) based on the median values of 75.5 yuan as the urban residents' willingness to pay, and 15.5 yuan as the willingness to pay of the rural residents, the externality of the cultivated land resources in Neihuang County, Henan Province, was estimated to be 11 649.5 yuan/hm2 (776.6 yuan/mu). Thus, more attention should be paid to the value of the externality of the cultivated land resources in estimating its total value, expropriation price, transaction price, and to policy making with respect to protection of cultivated land resources.

  12. Pose measurement method based on geometrical constraints

    Institute of Scientific and Technical Information of China (English)

    Zimiao Zhang; Changku Sun; Pengfei Sun; Peng Wang

    2011-01-01

    @@ The pose estimation method based on geometric constraints is studied.The coordinates of the five feature points in the camera coordinate system are calculated to obtain the pose of an object on the basis of the geometric constraints formed by the connective lines of the feature points and the coordinates of the feature points on the CCD image plane; during the solution process,the scaling and orthography projection model is used to approximate the perspective projection model.%The pose estimation method based on geometric constraints is studied. The coordinates of the five feature points in the camera coordinate system are calculated to obtain the pose of an object on the basis of the geometric constraints formed by the connective lines of the feature points and the coordinates of the feature points on the CCD image plane; during the solution process, the scaling and orthography projection model is used to approximate the perspective projection model. The initial values of the coordinates of the five feature points in the camera coordinate system are obtained to ensure the accuracy and convergence rate of the non-linear algorithm. In accordance with the perspective projection characteristics of the circular feature landmarks, we propose an approach that enables the iterative acquisition of accurate target poses through the correction of the perspective projection coordinates of the circular feature landmark centers. Experimental results show that the translation positioning accuracy reaches ±0.05 mm in the measurement range of 0-40 mm, and the rotation positioning accuracy reaches ±0.06° in the measurement range of 4°-60°.

  13. 如何培养学生的创新思维--以《植物学》“根”一节的教学过程为例%How to cultivate students′innovation thinking---based on the chapter "root"in"botany"curriculum as an example

    Institute of Scientific and Technical Information of China (English)

    吴晓霞; 丁海东; 徐小颖; 耿斌; 金银根

    2014-01-01

    The cultivation of innovative thinking ability is an important task of teaching in Colleges and Universities .One of the key factors influencing the cultivation of innovative talents is the process of culturing innovation thinking .The teaching process based on the chapter "root"in the "botany"curriculum was used as an example , through the "question string"teaching method , the mind map learning method and the concept of re-definition method and so on;more attention to the cultivation of students′innovative thinking a-bility in teaching process was emphazied to make students acquire knowledge and innovative thinking and learning methods scientifical -ly.%大学是培养创新型人才的重要场所,而培养学生的创新思维能力是培养创新型人才的关键。以《植物学》课程中的“根”的教学过程为例,探讨了“问题串”教学法、思维导图学习法以及概念重定义法在教学过程中的应用,实践表明,这样的教学方法有利于学生创新思维能力的培养,使学生在获得知识的同时学会科学的学习方法和创新思维方法。

  14. Cultivation of EFL Learners’Intercultural Competence

    Institute of Scientific and Technical Information of China (English)

    夏晨

    2012-01-01

      Nowadays, educators from home and abroad believe that learning a foreign language means learning a great deal of the foreign civilization and culture. And the utmost goal of language teaching is to cultivate EFL learners’communicative competence, but not merely the linguistic knowledge. Thus, teaching a foreign language is more than teaching new words, expressions and grammar rules, but should also incorporate the target culture elements into language knowledge. In order to improve EFL learners’English proficiency, teachers must make efforts to cultivate EFL learners’intercultural competence and promote their sensibility and adaptability to the differences between Chinese culture and the Western culture. Only by doing so, can EFL learners communicate with native speakers appropriately and smoothly. This pa-per proposes some effective teaching techniques and methods that can be applied in English classroom to cultivate Chinese EFL learners’in-tercultural competence.

  15. Satellite Formation based on SDDF Method

    Directory of Open Access Journals (Sweden)

    Yu Wang

    2014-04-01

    Full Text Available The technology of satellite formation flying has being a research focus in flight application. The relative position and velocity between satellites are basic parameters to achieve the control of formation flight during the satellite formation flying mission. In order to improve the navigation accuracy, a new filter different from Extended Kalman Filter (EKF should be adopted to estimate the errors of relative position and velocity, which is based on the nonlinearity of the kinetic model for the satellite formation flying. A nonlinear Divided Difference Filter (DDF based on Stirling interpolation formula was proposed in this paper. According to the linearity of the measurement equation for the filter, a simplified differential filter was designed by means of expanding the polynomial of the nonlinear system equation and linear approximating of the finite differential interpolation. Digital simulation experiment for the relative positioning of satellite formation flying was carried out. The result demonstrates that the filter proposed in this paper has a higher filtering accuracy, faster convergence speed and better stability. Compared with the EKF, the estimation accuracy of the relative position and velocity has improved by 77.1%and 47% respectively in the method of simplified DDF, which indicates the significance for practical applications. 

  16. [Cultivation of pathogenic free-living amoebae].

    Science.gov (United States)

    Peng, Heng; Zhu, Huai-Min

    2009-08-01

    The isolation and culture of pathogenic free-living amoebae are useful in the diagnosis and research. This review focuses on the methods of isolation and cultivation of pathogenic free-living amoebae, including sample treatment, culture conditions, passage culture, pathogen detection, and maintenance.

  17. Effect of cultivation measures on economic benefit of Larix olgesis pulp forest

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    According to the cultivating practice of Larix olgensis pulp plantation, IRR (Internal revenue rate) and NPV (Net present value) were taken as two economic indices to study the effect of cultivation measurements on economic benefit of Larix olgensis pulp forest. The results showed that the economic benefit of this type of forest is closely related to rotation and site class. Higher economic benefit could be obtained when the rotation is shorter and site class is higher. The planting density also had an obvious influence on economic benefit. On the base of assuring survival rate and conserving rate, the less the fee used in soil preparation and young growth tending is, the higher the economic benefit is. The influence of determined six cultivation meas-ures on economic benefit in sequence was the rotation-site class-density-management fee level-young growth tending in-tensity- soil preparation methods.

  18. 数学专业教学中学生创新能力的培养%Investigation on Methods for Cultivating Students' Creative Abilities in Teaching in Mathematics Major

    Institute of Scientific and Technical Information of China (English)

    钟文勇

    2012-01-01

    To cultivate creative talents is one of the main aims of education in mathematics major; the teaching is a key factor to realize the goal. In this work, we investigate the methods by which the teaching is organized effectively to train students' innovative abilities. We point out that the mathematical quali ties of teachers and the learning interest of students are premises to develop students ' innovative abili ties. We further analyze the functions of the teaching methods for cultivate students ' innovative abilities; and the methods involve analogy and conjecture and induction,the problemoriented ideas and the inte gration of modeling thinking into the teaching process ' and some concrete examples are given to demon strate that the methods discussed are advantageous to training students' innovative abilities.%培养创新型数学专业人才,是大学数学专业的培养目标之一,教学过程是实现这一目标的关键.以有效利用课堂教学进行学生创新能力培养为中心,以教师的数学素养和学生的学习兴趣为培养创新能力的前提,分析了类比、猜想及归纳、问题为导向和建模融入教学过程等教学方法在学生创新能力培养中的作用,并结合教学案例说明了这些教学思想有利于促进学生创新能力的培养.

  19. Long term cultivation of larger benthic Foraminifera

    Science.gov (United States)

    Wöger, Julia; Eder, Wolfgang; Kinoshita, Shunichi; Antonino, Briguglio; Carles, Ferrandes-Cañadell; Hohenegger, Johann

    2015-04-01

    Benthic Foraminifera are used in a variety of applications employing numerous different methods, i.e. ecological monitoring, studying the effects of ocean acidification, reconstructing palaeo-bathymetry or investigating palaeo-salinity and palaeo-temperature to name only a few. To refine our understanding of ecological influences on larger benthic foraminiferal biology and to review inferences from field observations, culture experiments have become an indispensable tool. While culture experiments on smaller benthic foraminifera have become increasingly frequent in the past century, reports of the cultivation of symbiont bearing larger Foraminifera are rare. Generally, cultivation experiments can be divided into two groups: Culturing of populations and cultivation of single specimens allowing individual investigation. The latter differ form the former by several restrictions resulting from the need to limit individual motility without abridging microenvironmental conditions in the Foraminiferans artificial habitat, necessary to enable the individual to development as unfettered as possible. In this study we present first experiences and preliminary results of the long-term cultivation of larger benthic Foraminifera conducted at the 'Tropical Biosphere Research Station Sesoko Island, University of the Ryukyus', Japan, trying to reproduce natural conditions as closely as possible. Individuals of three species of larger benthic Foraminifera (Heterostegina depressa, Palaeonummulites venosus and Operculina complanata) have been cultured since April 2014. At the time of the general assembly the cultivation experiments will have been going on for more than one year, with the aim to investigate growth rates, longevities and reproduction strategies for comparison with results statistically inferred from application of the of the 'natural laboratory' method. The most important factor influencing foraminiferal health and development was found to be light intensity and light

  20. Review on Cultivated Land Use and Protection Based on Three-Dimensional Perspective%基于“三维”视角的国内耕地利用与保护研究述评

    Institute of Scientific and Technical Information of China (English)

    张旺锋; 张瑞霞; 方晨; 易洋

    2012-01-01

    Rapid progress and prospect has made in cultivated land use and protection in China. With the passage of time, the study has gone through the transition of no-protection of cultivated land to strict-protection in research background, the change of qualitative analysis to quantitative analysis in research method,and the space-shifting of eastern developed area to the middle area which has the high speed of urbanization and the superior resource endowment Meanwhile, the eastern and middle area were ahead of western area in study on the same topic. The study shows that: 1) The research which has explored the impacts of economic and social factors on quantity protection of cultivated land is maturity;2) The study will be made deeply concerning the change of cultivated land's quality response to cultivated land use and the evolution of ecological environment;3) It is suggested for developing general protection of cultivated land and sustainable protection which has diversified goals and policies and ways as well in China.%分析和评述了国内耕地利用与保护的研究动态,对今后的研究方向进行了展望.国内有关耕地利用与保护的研究经历了研究背景由耕地无保护向耕地严格保护过渡,研究方法由定性为主向定量和计量为主转变,空间落点由东部经济发达地区向城镇化速度快、耕地资源禀赋条件好的中部地区转移.在某一研究论题断面上,东、中部地区始终先于西部地区.得出以下结论:经济、社会因子与耕地数量保护之间的作用机制研究已近饱和;耕地质量变动对耕地利用与地区生态环境演变的响应机制探讨亟待深入;我国应开展广义的耕地保护,走“目标多样化、政策多样化、途径多样化”的可持续耕地保护之路.

  1. Land Fiscalization and Cultivated Land Protection: Causal Relationship Based on Provincial Panel Data Analysis%土地财政与耕地保护——基于省际面板数据的因果关系分析

    Institute of Scientific and Technical Information of China (English)

    杜雪君; 黄忠华

    2009-01-01

    With rapid growth of economy, urbanization and industrialization, China' s cultivated land decreased 793 × 10~4 hm~2 from 1998 to 2008. Recent studies indicate land fiscalization is one of the important reasons for land decrease. The purpose of this study is to explore the relationship between land fiscalization and cultivated land change. Methods of Granger causality test, OLS, fixed effect model, random effect model and GMM model were used, based on all China provinces' panel data of cultivated land area, land fiscalization, GDP, population and urbanization level from 1999 to 2006. The Granger causality test result shows cultivated land and land fiscalization have mutual feedback effects. Empirical analysis reveals that land fiscaliztion is an important reason for cultivated land decrease, 1 percentage increase in land fiscaliztion would cause 0. 01 percentage decrease of cultivated land. Land fiscaliztion has two opposite impacts on cultivated land. On the one hand, land fiscaliztion could induce local governments to expand cultivated land occupation in pursuit of fiscal revenue growth and economic development. On the other hand, benefited from the land fiscaliztion growth, more capital investment in land consolidation could reduce land decrease rate.%论文利用1999-2006年我国31个省(直辖市、自治区,因资料所限,不包括台湾省及香港和澳门地区)的面板数据,采用格兰杰因果关系检验、普通最小二乘法、固定效应模型、随机效应模型和动态面板数据模型广义矩等来分析我国土地财政对耕地数量变化的影响.研究结果表明:①土地财政与耕地数量之间存在相互反馈作用;②土地财政是导致我国耕地数量减少的重要原因,其影响的弹性系数为-0.01;③土地财政对耕地保护具有正反两方面作用:一方面,土地财政刺激地方政府形成城市扩张冲动,导致耕地数量减少;另一方面,土地财政带来土地开发整理增加耕地面

  2. Effects of Temperature variations on the Super Fine Powderization of Korean Cultivated Wild Ginseng

    Directory of Open Access Journals (Sweden)

    Jin Ho Kim

    2006-12-01

    Full Text Available Objectives : The aim of this study was to find optimal conditions for producing red ginseng from cultivated wild ginseng using the Turbo Mill. Methods : Characteristics of powdered cultivated wild ginseng based on various temperature settings of the Turbo Mill were observed, and changes in the content was measured by HPLC for various ginsenosides. Results : 1. The diameter of cultivated wild ginseng powder ground by the Turbo Mill was around 10㎛. 2. As the temperature rose, presusre, Specific Mechanical Energy(SME, and density decreased, whileas Water Solubility Index(WSI increased. 3. As the temperature rose, super fine powder showed tendency to turn into dark brown. 4. Measuring content changes by HPLC, there was no detection of ginsenoside Rg3 and ginsenosideRg1, Rb1, and Rh2 concentrations decreased with increase in temperature. Conclusions : Super fine powder of cultivated wild ginseng produced by the Turbo Mill promotes easy absorption of effective ingredients by breaking the cell walls. Using this mechanism to produce red ginseng from cultivated wild ginseng, it yielded less than satisfactory results under the current experiment setup. Furtherresearches are needed to verify more suitable condition for the production of red ginseng.

  3. Comparative Study on the Human Driving Force of Cultivated Land and Construction Land Use Change in Hubei Province,China

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Change trend of cultivated land and construction land in Hubei Province is analyzed.The years 1998-2003 are the reduction period of cultivated land;the years 2004-2007 are the increase period of cultivated land;the years 1988-2002 are the slow growth period of construction land;and the years of 2003 and 2007 are the increase period of construction land.Based on related social economic statistical data in 1988-2007,human driving forces on cultivated land and construction land in Hubei Province are analyzed quantitatively and comparatively by using SPSS software and mathematical statistics method.Result shows that driving force factors have negative driving effect on cultivated land,and positive driving effect on construction land.Driving force factors have significant differences of driving effects on cultivated land and construction land.Three principal components affecting the changes of cultivated land and construction land are policy,population and economic development level,and gap between urban and rural living standards.The three principal components have different degrees of sensitivity on the two land use types and the effects direction are reverse.The other factor driving abilities are relatively weak,and these factors have relatively stronger driving force on construction land than that on cultivated land.It is put forward that Hubei Province should implement rational allocation of land resources and scientific and rational use in order to ensure land security and to realize the sustainable development of regional economy and society based on the intensive land use,the planning policy constraints,and the agricultural and industrial production.

  4. Exploration on cultivating students’ innovative thinking mode based on opening experiment%基于开放实验培养学生创新思维模式的探索

    Institute of Scientific and Technical Information of China (English)

    张立立; 杨文谷; 李大宇; 王明全

    2016-01-01

    For further training students’ innovation thinking and handling ability ,based on open experiment , this article proposes a new teaching method ,combined learning with self‐teaching ,to develop initiative and creativity .This article sets a specific example ,a car control system with Bluetooth technology based on App Inventor ,to guide students through the learning process . And it is organized evolutionarily ,from design requirements to inspection and acceptance . By finishing system inspection ,students are cultivated how to develop products ,how to do system debugging ,both are of great value in their future learning and working , which has been put in practice for a couple of years and received a good result .%为了进一步培养学生创新思维和实际动手能力,以开放实验为平台,采用以学带学的教学方式,充分发挥学生的主动性和创新性。该文以基于App Inventor技术的蓝牙遥控小车控制系统为例,从系统设计要求到系统调试验收进行了循序渐进的内容安排,使大部分学生能够从零基础开始逐渐深入地学习,并通过系统验收阶段的要求,培养了学生产品开发的思维方式,积累了一定的软硬件系统调试经验,对学生以后的学习和工作都有很大的帮助。

  5. Building a field- and model-based climatology of local water and energy cycles in the cultivated Sahel - annual budgets and seasonality

    Science.gov (United States)

    Velluet, C.; Demarty, J.; Cappelaere, B.; Braud, I.; Issoufou, H. B.-A.; Boulain, N.; Ramier, D.; Mainassara, I.; Charvet, G.; Boucher, M.; Chazarin, J.-P.; Oï, M.; Yahou, H.; Maidaji, B.; Arpin-Pont, F.; Benarrosh, N.; Mahamane, A.; Nazoumou, Y.; Favreau, G.; Seghieri, J.

    2014-12-01

    In the sub-Saharan Sahel, energy and water cycling at the land surface is pivotal for the regional climate, water resources and land productivity, yet it is still very poorly documented. As a step towards a comprehensive climatological description of surface fluxes in this area, this study provides estimates of long-term average annual budgets and seasonal cycles for two main land use types of the cultivated Sahelian belt: rainfed millet crop and fallow bush. These estimates build on the combination of a 7-year field data set from two typical plots in southwestern Niger with detailed physically based soil-plant-atmosphere modeling, yielding a continuous, comprehensive set of water and energy flux and storage variables over this multiyear period. In the present case in particular, blending field data with mechanistic modeling makes the best use of available data and knowledge for the construction of the multivariate time series. Rather than using the model only to gap-fill observations into a composite series, model-data integration is generalized homogeneously over time by generating the whole series with the entire data-constrained model simulation. Climatological averages of all water and energy variables, with associated sampling uncertainty, are derived at annual to sub-seasonal scales from the time series produced. Similarities and differences in the two ecosystem behaviors are highlighted. Mean annual evapotranspiration is found to represent ~82-85% of rainfall for both systems, but with different soil evaporation/plant transpiration partitioning and different seasonal distribution. The remainder consists entirely of runoff for the fallow, whereas drainage and runoff stand in a 40-60% proportion for the millet field. These results should provide a robust reference for the surface energy- and water-related studies needed in this region. Their significance and the benefits they gain from the innovative data-model integration approach are thoroughly discussed

  6. Region-based multisensor image fusion method

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Image fusion should consider the priori knowledge of the source images to be fused, such as the characteristics of the images and the goal of image fusion, that is to say, the knowledge about the input data and the application plays a crucial role. This paper is concerned on multiresolution (MR) image fusion. Considering the characteristics of the multisensor (SAR and FLIR etc) and the goal of fusion, which is to achieve one image in possession of the contours feature and the target region feature. It seems more meaningful to combine features rather than pixels. A multisensor image fusion scheme based on K-means cluster and steerable pyramid is presented. K-means cluster is used to segment out objects in FLIR images. The steerable pyramid is a multiresolution analysis method, which has a good property to extract contours information at different scales. Comparisons are made with the relevant existing techniques in the literature. The paper concludes with some examples to illustrate the efficiency of the proposed scheme.

  7. Subjective evidence based ethnography: method and applications.

    Science.gov (United States)

    Lahlou, Saadi; Le Bellu, Sophie; Boesen-Mariani, Sabine

    2015-06-01

    Subjective Evidence Based Ethnography (SEBE) is a method designed to access subjective experience. It uses First Person Perspective (FPP) digital recordings as a basis for analytic Replay Interviews (RIW) with the participants. This triggers their memory and enables a detailed step by step understanding of activity: goals, subgoals, determinants of actions, decision-making processes, etc. This paper describes the technique and two applications. First, the analysis of professional practices for know-how transferring purposes in industry is illustrated with the analysis of nuclear power-plant operators' gestures. This shows how SEBE enables modelling activity, describing good and bad practices, risky situations, and expert tacit knowledge. Second, the analysis of full days lived by Polish mothers taking care of their children is described, with a specific focus on how they manage their eating and drinking. This research has been done on a sub-sample of a large scale intervention designed to increase plain water drinking vs sweet beverages. It illustrates the interest of SEBE as an exploratory technique in complement to other more classic approaches such as questionnaires and behavioural diaries. It provides the detailed "how" of the effects that are measured at aggregate level by other techniques.

  8. 培养创新型人才的网络专业课程体系研究*%Research on the Curriculum System of Network Major Based on Innovative Talent Cultivation

    Institute of Scientific and Technical Information of China (English)

    汤海林; 单家凌

    2013-01-01

      网络专业是以培养应用性、复合型的创新人才为目标。分析网络专业的课程结构模型,对网络专业的课程结构、课时分配、实践教学与考核方式进行了阐述,提出以实践教学为主导、课程设计为支撑、理论教学为基础的立体式人才培养模式。%The talent cultivation's object of network major is to cultivate the innovative talents with ap-plied and comprehensive ability. Analyses the curriculum structure model of the network pro-fessional, details the curriculum structure, distribution of teaching hours, practical teaching and evalution model. Proposes a vertical talent cultivation model based on the leading of practical teaching, the surport of course desing and the foundation of theory teaching.

  9. Research on the Cultivation Model of Software Technology Specialty Based on CDIO Engineering Education Philosophy%基于CDIO理念的高职软件技术专业人才培养模式研究

    Institute of Scientific and Technical Information of China (English)

    贾家新

    2016-01-01

    In view of present situation of China′s higher vocational college of software technology talent cultivation it is necessary to learn the international advanced the concept of CDIO Engineering Education.Then,we need to establish our own talent cultivation model.The theory curriculum system teaching system and evaluation system were based on CDIO philosophy.Also,it is important to deepen software technology professional talent cultivation model.Meanwhile,weshould improve the quality of personnel training,and enhance highGquality software and technical talents.%针对我国高等职业院校软件技术专业人才培养的现状,借鉴国际先进的 CDIO 工程教育理念,分别从人才培养模式的构建、理论课程体系的构建、实践教学体系的构建、考核评价体系的构建等四个方面进行基于CDIO 理念软件技术专业人才培养的研究,探索软件技术专业人才培养模式的建设与改革,提高人才培养质量,为国家培养更多高素质技能应用型软件技术人才。

  10. 基于遥感和GIS的延边州耕地变化与驱动力分析%Study on the Changes of Cultivated Land and the Driving Forces in Yanbian Autonomous Prefecture Based on Remote Sensing and GIS

    Institute of Scientific and Technical Information of China (English)

    丁婧; 冯恒栋

    2013-01-01

    Based on the remote sensing data, the temporal and spatial change characteristics of cultivated land at the Yanbian Autonomous Prefecture during 1990 to 2010 were analyzed. The driving forces which caused the variation were also studied through the method of principal component. Result showed that the area of cultivated land keeps increasing in recent two decades, the increasing speed ups and then downs. The new increased land was mainly in the area of rich water and soil resources that was suitable for farming. The lost land mainly distributed in the edge of the original cultivated land as well as the urban peripheral. By principal component analysis, the driving factors were found to be social and economic development, popu-lation growth and the agricultural technology improvement. Finally, the measures for cultivated land and protection were proposed to make the cultivated land resources used safely and sustainably.%以延边朝鲜族自治州为研究区,基于遥感数据分析了1990~2010年该地区耕地的时空变化特征。并运用主成份分析方法探讨了影响耕地变化的驱动因子。结果表明:近20年来延边州耕地总量一直在增长,增加速度呈先快后慢的趋势。新增耕地主要位于各县市水土资源良好的宜耕地区,流失耕地主要分布在原有耕地的边缘以及城市的周边地区。通过主成分分析得出耕地变化的主要驱动因素是:社会经济发展、人口增长以及农业科技进步。在此基础上提出了保护耕地的措施,以确保该地区耕地资源得到安全和可持续利用。

  11. Cultivating the Synergetic Innovation Ability of College Students Based on the Synergetic Innovation Thought%基于协同创新思想的大学生协同创新能力培养

    Institute of Scientific and Technical Information of China (English)

    王海建

    2012-01-01

    在协同创新思想被广泛应用于社会各领域的环境下,培养大学生协同创新能力对于国家协同创新战略的实现、创新型人才的培养具有重要意义。大学生协同创新能力主要包括大学生的创新能力、协同意识、角色定位能力和协调沟通能力。基于协同创新思想.可从以下途径培养大学生的协同创新能力:优化大学生的能力结构,提升大学生协同创新素质;创新高校教学管理,搭建校内外协同创新平台;创新国家教育制度,为大学生协同创新能力培养提供制度保障。%In the environment of synergetic innovation thoughts have been widely applied in various fields of the society, cultivation of college students' synergetic innovation competence is of great significance for national synergetic innovation strategy implementation and the cultivation of innovative talents. College students' synergetic innovation ability mainly includes the innovation ability, synergetic awareness, role orientation ability and communication ability. We can take some ways to cultivate the synergetic innovation ability of college students based on synergetic innovation thought: promotion of college students' synergetic innovation quality from optimizing students' ability structure; innovation of teaching and management in college from building the synergetic innovation platform inside and outside in university; innovation of the national system to provide a system guarantee for cultivating the college students' synergetic in- novation ability.

  12. [Selectivity rank regionalization of Paeonia lactiflora based on fuzzy method].

    Science.gov (United States)

    Lv, Jinrong; Guo, Lanping; Huang, Luqi; Liang, Liuke; Sun, Yuzhang; Zhang, Xiaobo; Han, Xiaoli; Zhang, Hongjun

    2009-04-01

    For optimal adaptive cultivation region selection, we used ecology factors characterized Duolun region as model area to carry out the adaptive habitat division of Paeonia lactiflora. Similar priority comparison of ecology factors.in 91 cities were calculated by Fuzzy methods, then, distance of the ecology factors were transferred to spacial model by geography information system (,GIS) and modified by soil utilization map of China. The results showed that P. lactiflora were mainly distributed in the Daxing'an Mountain, Changbaishan and qinling range which were divided into six grades of suitable regions belonging to three geographical distributed units. The most similar areas to Duolun were Huade, Xilinhaote, Suolun and Zhangbei. P. lactiflora's distribution and quality are relevant with longitude and latitude, and temperature and rainfall.

  13. Application of "Three-phase Cultivation Method" in Entrepreneurship Education of Post-1990 College Students%"三段式培养法"在90后大学生创业教育中的应用

    Institute of Scientific and Technical Information of China (English)

    郭姗

    2015-01-01

    通过对90后大学生创业意见和现状的调研,了解90后大学生群体对于创业教育和指导工作的需求,总结归纳出适合90后大学生的创业教育"三段式培养法",提出创业教育"共同体"的概念.%According to the survey of the entrepreneurship opinions and status of the post-1990 college students,the students' demands for entrepreneurship education and guidance is known,the "Three-phase Cultivation Method" fit to post-1990 college students is summarized,and the concept of community for entrepreneurship education is put forward.

  14. Triptycene-based ladder monomers and polymers, methods of making each, and methods of use

    KAUST Repository

    Pinnau, Ingo

    2015-02-05

    Embodiments of the present disclosure provide for a triptycene-based A-B monomer, a method of making a triptycene-based A-B monomer, a triptycene-based ladder polymer, a method of making a triptycene-based ladder polymers, a method of using triptycene-based ladder polymers, a structure incorporating triptycene-based ladder polymers, a method of gas separation, and the like.

  15. DNA-based methods of geochemical prospecting

    Science.gov (United States)

    Ashby, Matthew

    2011-12-06

    The present invention relates to methods for performing surveys of the genetic diversity of a population. The invention also relates to methods for performing genetic analyses of a population. The invention further relates to methods for the creation of databases comprising the survey information and the databases created by these methods. The invention also relates to methods for analyzing the information to correlate the presence of nucleic acid markers with desired parameters in a sample. These methods have application in the fields of geochemical exploration, agriculture, bioremediation, environmental analysis, clinical microbiology, forensic science and medicine.

  16. NMR and GC-MS based metabolic profiling and free-radical scavenging activities of Cordyceps pruinosa mycelia cultivated under different media and light conditions.

    Directory of Open Access Journals (Sweden)

    Taek-Joo Oh

    Full Text Available Variation of metabolic profiles in Cordyceps pruinosa mycelia cultivated under various media and light conditions was investigated using 1H nuclear magnetic resonance (NMR analysis and gas chromatography mass spectrometry (GC-MS with multivariate statistical analysis. A total of 71 metabolites were identified (5 alcohols, 21 amino acids, 15 organic acids, 4 purines, 3 pyrimidines, 7 sugars, 11 fatty acids, and 5 other metabolites by NMR and GC-MS analysis. The mycelia grown in nitrogen media and under dark conditions showed the lowest growth and ergosterol levels, essential to a functional fungal cell membrane; these mycelia, however, had the highest levels of putrescine, which is involved in abiotic stress tolerance. In contrast, mycelia cultivated in sabouraud dextrose agar with yeast extract (SDAY media and under light conditions contained relatively higher levels of fatty acids, including valeric acid, stearic acid, lignoceric acid, myristic acid, oleic acid, palmitoleic acid, hepadecenoic acid, and linoleic acid. These mycelia also had the highest phenolic content and antioxidant activity, and did not exhibit growth retardation due to enhanced asexual development caused by higher levels of linoleic acid. Therefore, we suggested that a light-enriched environment with SDAY media was more optimal than dark condition for cultivation of C. pruinosa mycelia as biopharmaceutical or nutraceutical resources.

  17. NMR and GC-MS Based Metabolic Profiling and Free-Radical Scavenging Activities of Cordyceps pruinosa Mycelia Cultivated under Different Media and Light Conditions

    Science.gov (United States)

    Oh, Taek-Joo; Hyun, Sun-Hee; Lee, Seul-Gi; Chun, Young-Jin; Sung, Gi-Ho; Choi, Hyung-Kyoon

    2014-01-01

    Variation of metabolic profiles in Cordyceps pruinosa mycelia cultivated under various media and light conditions was investigated using 1H nuclear magnetic resonance (NMR) analysis and gas chromatography mass spectrometry (GC-MS) with multivariate statistical analysis. A total of 71 metabolites were identified (5 alcohols, 21 amino acids, 15 organic acids, 4 purines, 3 pyrimidines, 7 sugars, 11 fatty acids, and 5 other metabolites) by NMR and GC-MS analysis. The mycelia grown in nitrogen media and under dark conditions showed the lowest growth and ergosterol levels, essential to a functional fungal cell membrane; these mycelia, however, had the highest levels of putrescine, which is involved in abiotic stress tolerance. In contrast, mycelia cultivated in sabouraud dextrose agar with yeast extract (SDAY) media and under light conditions contained relatively higher levels of fatty acids, including valeric acid, stearic acid, lignoceric acid, myristic acid, oleic acid, palmitoleic acid, hepadecenoic acid, and linoleic acid. These mycelia also had the highest phenolic content and antioxidant activity, and did not exhibit growth retardation due to enhanced asexual development caused by higher levels of linoleic acid. Therefore, we suggested that a light-enriched environment with SDAY media was more optimal than dark condition for cultivation of C. pruinosa mycelia as biopharmaceutical or nutraceutical resources. PMID:24608751

  18. Surface modification of closed plastic bags for adherent cell cultivation

    Science.gov (United States)

    Lachmann, K.; Dohse, A.; Thomas, M.; Pohl, S.; Meyring, W.; Dittmar, K. E. J.; Lindenmeier, W.; Klages, C.-P.

    2011-07-01

    In modern medicine human mesenchymal stem cells are becoming increasingly important. However, a successful cultivation of this type of cells is only possible under very specific conditions. Of great importance, for instance, are the absence of contaminants such as foreign microbiological organisms, i.e., sterility, and the chemical functionalization of the ground on which the cells are grown. As cultivation of these cells makes high demands, a new procedure for cell cultivation has been developed in which closed plastic bags are used. For adherent cell growth chemical functional groups have to be introduced on the inner surface of the plastic bag. This can be achieved by a new, atmospheric-pressure plasma-based method presented in this paper. The method which was developed jointly by the Fraunhofer IST and the Helmholtz HZI can be implemented in automated equipment as is also shown in this contribution. Plasma process gases used include helium or helium-based gas mixtures (He + N2 + H2) and vapors of suitable film-forming agents or precursors such as APTMS, DACH, and TMOS in helium. The effect of plasma treatment is investigated by FTIR-ATR spectroscopy as well as surface tension determination based on contact angle measurements and XPS. Plasma treatment in nominally pure helium increases the surface tension of the polymer foil due to the presence of oxygen traces in the gas and oxygen diffusing through the gas-permeable foil, respectively, reacting with surface radical centers formed during contact with the discharge. Primary amino groups are obtained on the inner surface by treatment in mixtures with nitrogen and hydrogen albeit their amount is comparably small due to diffusion of oxygen through the gas-permeable bag, interfering with the plasma-amination process. Surface modifications introducing amino groups on the inner surface turned out to be most efficient in the promotion of cell growth.

  19. Application of Particle Fixation Cultivate Dish Blank Contrast Method in Microbial Limit Test%颗粒定置琼脂基空白对照法在微生物限度检查中的应用

    Institute of Scientific and Technical Information of China (English)

    张娅琴; 张雅稚; 李家琪

    2011-01-01

    目的:研究一种简单易行、快捷、准确的辨别微生物限度检查中菌落与药物颗粒的方法.方法:取琼脂粉制备成颗粒定置琼脂基为空白对照,随样品微生物限度检测时平行操作,计数时以试验培养基上的菌落数减去空白对照的颗粒数,即得样品中的实际菌落数;对所制颗粒定置琼脂基进行标准菌生长试验;取本院自制样品对该法进行验证试验,并与《中国药典》显微镜检法进行比较.结果:标准菌在颗粒定置琼脂基上未见菌生长;本院所制各供试液以颗粒定置琼脂基为空白对照检查所得菌检结果与显微镜检法结果一致,相符率为100%,但前者每批检测时间平均为0.40 min,后者为14.12 min.结论:用颗粒定置琼脂基作空白对照可获得与显微镜检法相同的结果,且前法经证明设计科学,操作简单、快捷,结果准确.%OBJECTIVE: To study a kind of simple, rapid, accurate test method to identify the microbial limits detection of bacterial colonies and drug granules. METHODS: Particle fixation cultivate dish was prepared as blank control using agar powder and microbial limit detection of samples was conducted. The number of blank control colonies was subtracted from colonies of nutrition medium to obtain the actual number of bacterial colonies; standard bacterial growth test of prepared particle fixation cultivate dish was conducted; validation of standard bacterial growth test for self-made samples of our hospital was carried out and it was compared with microscopic examination counting process stated in Chinese Pharmacopeia. RESULTS: No standard bacterial growth was observed on the particle fixation cultivate dish. The growth test of self-made test solution of our hospital was in accordance with the result of microscopic examination to the extent of 100%. The time spending on determination was 0.40 min for the former and 14.12 min for the latter. CONCLUSION: The result of microbial

  20. Effects of different mulching materials on rice yield in transplanting field with semiarid cultivation method%半干旱栽培稻田不同秸秆覆盖材料的产量效应

    Institute of Scientific and Technical Information of China (English)

    陶诗顺; 王学春; 徐健蓉

    2012-01-01

    panicles by 4% -8% , weight of 1000 grains by0.6% - 1.7% under straw mulching treatments, compared with that under no mulching treatment. (2) Integrated with semiarid cultivation method, straw mulching method could significantly increase rice yield in transplanting field in the northwest region of Sichuan Province. The average yield of Cangyou 725 and Dyou 363 under mulching treatments with rapeseed husk, winter wheat husk and winter wheat straw increased by 10.2% , 7.0% and 7. 7% respectively, compared with that under no mulching treatment.

  1. Multifractal Framework Based on Blanket Method

    Science.gov (United States)

    Paskaš, Milorad P.; Reljin, Irini S.; Reljin, Branimir D.

    2014-01-01

    This paper proposes two local multifractal measures motivated by blanket method for calculation of fractal dimension. They cover both fractal approaches familiar in image processing. The first two measures (proposed Methods 1 and 3) support model of image with embedded dimension three, while the other supports model of image embedded in space of dimension three (proposed Method 2). While the classical blanket method provides only one value for an image (fractal dimension) multifractal spectrum obtained by any of the proposed measures gives a whole range of dimensional values. This means that proposed multifractal blanket model generalizes classical (monofractal) blanket method and other versions of this monofractal approach implemented locally. Proposed measures are validated on Brodatz image database through texture classification. All proposed methods give similar classification results, while average computation time of Method 3 is substantially longer. PMID:24578664

  2. New deghosting method based on generalized triangulation

    Institute of Scientific and Technical Information of China (English)

    Bai Jing; Wang Guohong; Xiu Jianjuan; Wang Xiaobo

    2009-01-01

    A new deghosting method baaed on the generalized triangulation is presented. First, two intersection points corresponding to the emitter position are obtained by utilizing two azimuth angles and two elevation angles from two jammed 3-D radars (or 2-D passive sensors). Then, hypothesis testing baaed deghosting method in the multiple target scenarios is proposed using the two intersection points. In order to analyze the performance of the proposed method, the correct association probability of the true targets and the incorrect association probability of the ghost targets are defined. Finally, the Monte Carlo simulations are given for the proposed method compared with the hinge angle method in the cases of both two and three radars. The simulation results show that the proposed method has better performance than the hinge angle method in three radars case.

  3. Study on Economic growth, Urbanization and Sustainable Utilization of Cultivated Land in China

    Institute of Scientific and Technical Information of China (English)

    Wu Qun; Guo Guancheng; Fan Li

    2004-01-01

    By means of the dynamic regression model, this paper analyzes the relationships among economic growth, urbanization and changes of cultivated land in China, finds that the ratio of cultivated land occupied by economic growth is decreasing with social and economic growth. And,based on that, some policy suggestions on how to promote the sustainable use of cultivated land in China are put forward.

  4. Characterization of quantity-quality grade conversion coefficient of supplementary cultivated land in Jianghan Plain, China

    Science.gov (United States)

    Hu, Shougeng; Wang, Jue; Wang, Zhanqi

    2010-11-01

    Relating supplementary cultivated land (SCL) quantity and quality with the occupied cultivated land (OCL) grade as well as realizing conversion between them are crucial to keep the balance between SCL and OCL, to protect the limited farming resources, to guarantee the security of national food, to harmonize the relationship between human and land resources, and to promote sustainable economic development. The Jianhan plain, one of China's grain production bases, was chosen for a case study to quantify the relationships between the grade index of farmland utilization and standard food output based on the resulting grade of agricultural land. Linear regression is used to model the relationships for 2778 samples from the grade of agricultural land obtained by field mapping. The average of grade index for different utilization grades was estimated by the area weighted average method. The results demonstrate that quantity-quality grade conversion coefficient of supplementary cultivated land, and the method used in this study are powerful tools to quantify the relationship between the quantity and quality of the SCL and the grade and realize conversion of OCL. Theses results can be used for protecting the quantity and quality of cultivated land and optimizing design for land consolidation project as well as determining the reasonable scale and layout of land use planning in the Jianghan plain.

  5. Origin of worldwide cultivated barley revealed by NAM-1 gene and grain protein content

    Directory of Open Access Journals (Sweden)

    Yonggang eWang

    2015-09-01

    Full Text Available The origin, evolution and distribution of cultivated barley provides powerful insights into the historic origin and early spread of agrarian culture. Here, population-based genetic diversity and phylogenetic analyses were performed to determine the evolution and origin of barley and how domestication and subsequent introgression have affected the genetic diversity and changes in cultivated barley on a worldwide scale. A set of worldwide cultivated and wild barleys from Asia and Tibet of China were analyzed using the sequences for NAM-1 gene and gene-associated traits-GPC (grain protein content. Our results showed Tibetan wild barley distinctly diverged from Near Eastern barley, and confirmed that Tibet is one of the origin and domestication centers for cultivated barley, and in turn supported a polyphyletic origin of domesticated barley. Comparison of haplotype composition among geographic regions revealed gene flow between Eastern and Western barley populations, suggesting that the Silk Road might have played a crucial role in the spread of genes. The GPC in the 118 cultivated and 93 wild barley accessions ranged from 6.73% to 12.35% with a mean of 9.43%. Overall, wild barley had higher averaged GPC (10.44% than cultivated barley. Two unique haplotypes (Hap2 and Hap7 caused by a base mutations (at position 544 in the coding region of the NAM-1 gene might have a significant impact on the GPC. SNPs and haplotypes of NAM-1 associated with GPC in barley could provide a useful method for screening GPC in barley germplasm. The Tibetan wild accessions with lower GPC could be useful for malt barley breeding

  6. Functional State Modelling of Cultivation Processes: Dissolved Oxygen Limitation State

    Directory of Open Access Journals (Sweden)

    Olympia Roeva

    2015-04-01

    Full Text Available A new functional state, namely dissolved oxygen limitation state for both bacteria Escherichia coli and yeast Saccharomyces cerevisiae fed-batch cultivation processes is presented in this study. Functional state modelling approach is applied to cultivation processes in order to overcome the main disadvantages of using global process model, namely complex model structure and a big number of model parameters. Alongwith the newly introduced dissolved oxygen limitation state, second acetate production state and first acetate production state are recognized during the fed-batch cultivation of E. coli, while mixed oxidative state and first ethanol production state are recognized during the fed-batch cultivation of S. cerevisiae. For all mentioned above functional states both structural and parameter identification is here performed based on experimental data of E. coli and S. cerevisiae fed-batch cultivations.

  7. 基于CAN的能源微藻培养监控系统的设计与实现%Design and Realization of Energy Microalgae Cultivating and Monitoring System Based on CAN

    Institute of Scientific and Technical Information of China (English)

    郭威; 蔡强; 张延军; 徐勤利; 王金辉

    2012-01-01

    针对能源微藻培养监控系统具体需求,选用CAN总线构建主从式总线网络,研制了一种基于STM32F107来构建采集从节点和控制从节点的软硬件设计方法.监控主机采用组态王6.53来实现现场数据的实时显示、数据库存储、实验流程组态和现场情况的Web发布.经调试结果表明:本监控系统可在波特率为9 600 bit/s下正常运行,有较高的稳定性和功能可扩展性,在生物能源领域中有广阔的应用前景.%In view of specific requirement of monitoring system in energy microalgae cultivating process, using CAN bus to build the network of master-slave mode, a software and hardware method of building slave nodes for collecting and controlling is developed, which is based on STM32F107. Using King View 6.53 for central monitor can display real-time data,store the data in database,configure the experimental process and issue the situation report on Web server. The testing result shows that the monitoring system can run under the band rate of 9 600 bit/s normally, high stable and scalable. It has a wide application prospects in the bio-energy area.

  8. 计量经济学教学模式的改革与能力培养%The Reform of the Teaching Methods for Econometrics and the Capacity Cultivation

    Institute of Scientific and Technical Information of China (English)

    孙文杰

    2015-01-01

    计量经济学是本科院校财经类或经管类专业的核心基础课程之一,对学生创新能力的培养和综合素质的提高具有十分重要的作用。本文围绕计量经济学教学中需要培养的关键能力,从培养学生独立思考的能力,运用恰当的计量模型对经济现象或经济问题进行抽象的能力以及比较和评价不同计量经济理论方法的能力等方面,提出计量经济学教学模式改革的对策和思路,为提高计量经济学的教学效果提供有益借鉴。%The econometrics is one of core basic courses of the major of the finance and economics or the economics and man-agement, which is most important for the cultivation of the stu-dents' innovation capacity and the improvement of the overall quality. Around the key capacity that should be acquired in teaching econometrics, the author analyzes the cultivation of the students' independent thinking capacity, the capacity of applying proper econometrics models to represent the economic phenome-na and economic problems and the capacity of comparing and e-valuating different econometrics theories and models, and puts forward the proposals and ideas for the reform of the teaching methods of econometrics in order to enhance the teaching effect of econometrics.

  9. Research on the Progress of Comprehensive Evaluation Method Application in Clinical Competence Cultivation of Medical Students%综合评估法在医学生临床能力培养中的应用进展研究

    Institute of Scientific and Technical Information of China (English)

    胥娇; 张东华; 董靖竹; 周佳; 薄红

    2014-01-01

    Cultivation of medical students’ clinical competence is one of the important issues of com-mon concern to domestic and foreign medical education , w hile a sound comprehensive evaluation method of medical students’ clinical ability is the key to it .In this paper ,through the systematically study concepts ,connotations ,applications in the evaluation of clinical ability of medical students and its advantages of OSCE ,MiniCEX ,concept 360 feedback ,which are widely used in evaluation of medical students’ clinical competence ,and provide ideas in building a comprehensive evaluation feed-back system in clinical competence cultivation of medical students .%医学生临床能力培养是国内外医学教育界普遍关注的重要问题之一,而健全完善的医学生临床能力综合评估反馈体系是医学生能力培养的关键。本文通过系统的文献回顾诠释在医学生临床能力评价中各评价方法的应用特点,通过对其广泛应用的OSCE、Mini-CEX、360°评价方法的概念、内涵,在医学生临床能力评价中的应用进展以及应用优势的阐述,以期为医学生临床能力综合评估反馈体系的建立提供思路和借鉴。

  10. Primary exploration of the application of case based learning method in clinical probation teaching of the integrated curriculum of hematology

    Institute of Scientific and Technical Information of China (English)

    Zi-zhen XU; Ye-fei WANG; Yan WANG; Shu CHENG; Yi-qun HU; Lei DING

    2015-01-01

    Objective To explore the application and the effect of the case based learning(CBL)method in clinical probation teaching of the integrated curriculum of hematology among eight-year-program medical students.Methods The CBL method was applied to the experimental group,and the traditional approach for the control group.After the lecture,a questionnaire survey was conducted to evaluate the teaching effect in the two groups.Results The CBL method efficiently increased the students’interest in learning and autonomous learning ability,enhanced their ability to solve clinical problems with basic theoretic knowledge and cultivated their clinical thinking ability.Conclusion The CBL method can improve the quality of clinical probation teaching of the integrated curriculum of hematology among eight-year-program medical students.

  11. A microfluidic based optical particle detection method

    Science.gov (United States)

    Dou, James; Chen, Lu; Nayyar, Rakesh; Aitchison, Stewart

    2012-03-01

    An optical particle detection and analysis method is presented. This method combines the capillary microfluidics, integrated optics and novel image acquisition and analysis algorithms to form the basis of a portable or handheld cytometer instrument. Experimental results provided shows the testing results are closely matched with conventional flow cytometer data.

  12. Glass bead cultivation of fungi

    DEFF Research Database (Denmark)

    Droce, Aida; Sørensen, Jens Laurids; Giese, H.

    2013-01-01

    Production of bioactive compounds and enzymes from filamentous fungi is highly dependent on cultivation conditions. Here we present an easy way to cultivate filamentous fungi on glass beads that allow complete control of nutrient supply. Secondary metabolite production in Fusarium graminearum and...

  13. Micrometeorological principles of protected cultivation

    Science.gov (United States)

    Protected cultivation is a broad term commonly used among producers of specialty crops. Techniques can range from complex fixed structures to field site selection, to straightforward cultural practices in the field. This introduction to the ASHS workshop "Protected cultivation for fruit crops" consi...

  14. Cultivating Technological Innovation in the Mind

    Directory of Open Access Journals (Sweden)

    PROF ANYATA BENEDICT U.(Ph.D

    2015-11-01

    Full Text Available Cultivating innovation in the mind is imperative to successfully move technology to the next level. Technology is growing rapidly in developed countries and has almost reached its peak. Developing countries and continents like Africa on the other hand are now seen as a breeding ground to explore in technological innovation. Researchers and entrepreneurs are shifting base to Africa where they can achieve maximum profit resulting from under development of the continent. Reefs, city and web are used to explain the best way to cultivate innovation. The city and web are such engines of new innovation because both environments are powerfully suited for the creation, diffusion and adoption of good ideas. The seven keys of cultivating innovation include tropical humidity, illiteracy, poor power surge of electronic use for research, good concept of the liquid networks, slow hunches, serendipity, acceptance of error in cultivating innovation, Exaptation, coffee breaks, application of programming interface and the use of quadrant as a tool. These keys are recommended to be put to use by every individual who has the mindset of driving technological innovation to the next level.

  15. On Cultivating Students' Motivation in Second Language Acquisition

    Institute of Scientific and Technical Information of China (English)

    沈小平

    2009-01-01

    This paper attempts to explore the cultivation of motivation in second language acquisition based on shedding new light on the definition, the importance, and the classification of learners' motivation in second language acquisition.

  16. Mathematic Teaching Strategy Based on Teamwork Spirit Cultivation%基于合作意识培养的数学教学策略探析

    Institute of Scientific and Technical Information of China (English)

    雷兴辉

    2011-01-01

    针对以就业为导向的职业院校数学教学,探讨在教学中培养学生合作意识和合作能力的策略,以期为学生就业做好数学方面的准备.%Aiming at employment-oriented mathematic teaching in vocational college, the article explored the strategy of cultivating students' teamwork spirit and team work ability in teaching, in order to prepare for students' employment.

  17. Labor Cost Analysis for Pome Production in Different Cultivation Modes in Hebei Province

    Institute of Scientific and Technical Information of China (English)

    Shaoyan; ZHOU; Yu; HUANG; Cui; YAO; Runhang; LU; Xingfang; LIU

    2013-01-01

    Taking the traditional fruit pear as the example, this paper analyzes labor cost for pome production in Hebei Province, based on the representative cases and the research of pome production in different cultivation modes. Firstly, it conducts cost analysis for medium-density pome production in Xinji City, focusing on the comparison of the costs for the main production labor in standard thin planting mode and dwarf close planting mode. According to the research results, labor cost has a great influence on the total production cost of pome. The methods to reduce labor cost include: adopting dwarfing rootstock close planting and intensively efficient pome cultivation method; simplifying the pruning method when matching up the shape of tree; improving soil by the methods of natural grasses and addition of organic materials, and increasing mechanized operation.

  18. Development and application of a quantitative method based on LC-QqQ MS/MS for determination of steviol glycosides in Stevia leaves.

    Science.gov (United States)

    Molina-Calle, M; Sánchez de Medina, V; Delgado de la Torre, M P; Priego-Capote, F; Luque de Castro, M D

    2016-07-01

    Stevia is a currently well-known plant thanks to the presence of steviol glycosides, which are considered as sweeteners obtained from a natural source. In this research, a method based on LC-MS/MS by using a triple quadrupole detector was developed for quantitation of 8 steviol glycosides in extracts from Stevia leaves. The ionization and fragmentation parameters for selected reaction monitoring were optimized. Detection and quantitation limits ranging from 0.1 to 0.5ng/mL and from 0.5 to 1ng/mL, respectively, were achieved: the lowest attained so far. The steviol glycosides were quantified in extracts from leaves of seven varieties of Stevia cultivated in laboratory, greenhouse and field. Plants cultivated in field presented higher concentration of steviol glycosides than those cultivated in greenhouse. Thus, the way of cultivation clearly influences the concentration of these compounds. The inclusion of branches together with leaves as raw material was also evaluated, showing that this inclusion modifies, either positively or negatively, the concentration of steviol glycosides.

  19. 综合运用多种方法,有效培养学生应用写作语言风格%Comprehensive use of various methods, effectively cultivate students' practical writing language style

    Institute of Scientific and Technical Information of China (English)

    王颖

    2013-01-01

    In the application writing teaching, the language style is the difficult point of teaching, its reason is no concept of applied writing language style of students in learning, in order to solve this problem, in this paper, the theory of implicit learning and explicit learning are introduces into the teaching, and put forward comprehensive use of reading method, comparative method and practice method in teaching links, in order to cultivate students' practical writing language style.%  在应用写作的教学中,应用文的语言风格是教学的难点,其原因是学生在学习中往往对应用写作的语言风格没有概念,针对这一问题,本文将内隐学习和外显学习的相关理论引入教学,并提出在教学的前、中、后等环节,综合运用阅读法、比较法和实践训练法,以期有效培养学生的应用写作语言风格。

  20. New ITF measure method based on fringes

    Science.gov (United States)

    Fang, Qiaoran; Liu, Shijie; Gao, Wanrong; Zhou, You; Liu, HuanHuan

    2016-01-01

    With the unprecedented developments of the intense laser and aerospace projects', the interferometer is widely used in detecting middle frequency indicators of the optical elements, which put forward very high request towards the interferometer system transfer function (ITF). Conventionally, the ITF is measured by comparing the power spectra of known phase objects such as high-quality phase step. However, the fabrication of phase step is complex and high-cost, especially in the measurement of large-aperture interferometer. In this paper, a new fringe method is proposed to measure the ITF without additional objects. The frequency was changed by adjusting the number of fringes, and the normalized transfer function value was measured at different frequencies. The ITF value measured by fringe method was consistent with the traditional phase step method, which confirms the feasibility of proposed method. Moreover, the measurement error caused by defocus was analyzed. The proposed method does not require the preparation of a step artifact, which greatly reduces the test cost, and is of great significance to the ITF measurement of large aperture interferometer.

  1. Model-based methods for linkage analysis.

    Science.gov (United States)

    Rice, John P; Saccone, Nancy L; Corbett, Jonathan

    2008-01-01

    The logarithm of an odds ratio (LOD) score method originated in a seminal article by Newton Morton in 1955. The method is broadly concerned with issues of power and the posterior probability of linkage, ensuring that a reported linkage has a high probability of being a true linkage. In addition, the method is sequential so that pedigrees or LOD curves may be combined from published reports to pool data for analysis. This approach has been remarkably successful for 50 years in identifying disease genes for Mendelian disorders. After discussing these issues, we consider the situation for complex disorders where the maximum LOD score statistic shares some of the advantages of the traditional LOD score approach, but is limited by unknown power and the lack of sharing of the primary data needed to optimally combine analytic results. We may still learn from the LOD score method as we explore new methods in molecular biology and genetic analysis to utilize the complete human DNA sequence and the cataloging of all human genes.

  2. 基于碳循环的黄淮海平原耕地固碳功能研究%Carbon sequestration function of cultivated land use system based on the carbon cycle for the Huang-Huai-Hai Plain

    Institute of Scientific and Technical Information of China (English)

    陈丽; 郝晋珉; 王峰; 尹钰莹; 高阳; 段文凯; 杨君

    2016-01-01

    Cultivated land carbon sequestration as an effective supplement to the terrestrial ecosystem is recognized;however,research on the carbon sequestration function of cultivated land has been conducted from the perspective of ecology.For example,carbon uptake and carbon release of vegetation and arable soil and carbon balance in agro-ecosystems is often a focus and there is a lack of research into the carbon sequestration of cultivated land from the viewpoint of land science.Here we built a basic research framework for the carbon sequestration function of cultivated land use for the Huang-Huai-Hai Plain based on the carbon cycle process of cultivated land use system theory.Then the carbon sequestration function was evaluated from three aspects of carbon sequestration ability,carbon sequestration efficiency and carbon sequestration dominance.The results suggest that the carbon sequestration function of cultivated land use can be known from four aspects:natural carbon sequestration ability,ideal carbon sequestration ability,practical carbon sequestration ability and regional carbon sequestration ability.The carbon sequestration of cultivated land use systems of the Huang-Huai-Hai Plain is a carbon sink.The carbon sequestration efficiency and carbon sequestration dominance degree are 2.5 and 0.85 respectively.The carbon sequestration function of cultivated land use system plays an irreplaceable role on the Huang-Huai-Hai Plain,which should be a prominent function in this area.This study provides a new way of thinking about ecological function theory research for cultivated land,and support for promoting the ecological benefits of cultivated land,mitigating climate warming,and administering cultivated land ecology interregional compensation.%耕地固碳作为陆地主要生态系统固碳的有效补充,越来越引起学术界的广泛关注.但是,目前耕地固碳功能研究多从生态学角度研究耕地植被、土壤等碳吸收、碳释放

  3. On Research-Based Learning and the Cultivation of A-gricultural Science College Students' Professional Identity%论研究型学习与农科大学生专业认同感的培养

    Institute of Scientific and Technical Information of China (English)

    朱俊; 刘震; 付庆灵; 黄巧云; 胡红青

    2013-01-01

    Agricultural science college students are the hope of a-gricultural and rural development. Professional identity influ-ences students' learning initiative and future occupational plan-ning, so it is an important index measuring the quality of talent cultivation. Research-based learning can stimulate and develop students' professional interest. Through analyzing the relationship among research-based learning, learning interest and profession-al identity, this paper explores the necessity and feasibility of cultivating agricultural science college students' professional i-dentity through research-based learning.%  农科大学生是农业和农村发展的希望。专业认同影响着学生对所学专业的学习动力和对未来的职业规划,是衡量人才培养质量的重要指标之一。研究型学习以培养学生在学习过程中的主体性和探索性为特征,可以激发和发展专业兴趣。本文拟通过分析研究型学习、专业兴趣、专业认同之间的关系,探讨通过研究型学习培养农科大学生专业认同感的必要性和可行性。

  4. LEVEL SET METHODS BASED ON DISTANCE FUNCTION

    Institute of Scientific and Technical Information of China (English)

    王德军; 唐云; 于洪川; 唐泽圣

    2003-01-01

    Some basic problems on the level set methods were discussed, such as the method used to preserve the distance function, the existence and uniqueness of solution for the level set equations. The main contribution is to prove that in a neighborhood of the initial zero level set, the level set equations with the restriction of the distance function have a unique solution, which must be the signed distance function with respect to the evolving surface. Some skillful approaches were used: Noticing that any solution for the original equation was a distance function, the original level set equations were transformed into a simpler alternative form. Moreover, since the new system was not a classical one, the system was transforned into an ordinary one, for which the implicit function method was adopted.

  5. AN EVEN COMPONENT BASED FACE RECOGNITION METHOD

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    This paper presents a novel face recognition algorithm. To provide additional variations to training data set, even-odd decomposition is adopted, and only the even components (half-even face images) are used for further processing. To tackle with shift-variant problem,Fourier transform is applied to half-even face images. To reduce the dimension of an image,PCA (Principle Component Analysis) features are extracted from the amplitude spectrum of half-even face images. Finally, nearest neighbor classifier is employed for the task of classification. Experimental results on ORL database show that the proposed method outperforms in terms of accuracy the conventional eigenface method which applies PCA on original images and the eigenface method which uses both the original images and their mirror images as training set.

  6. Teaching Reform and Practice of Nursing Specialty Courses Based on Cultivation of Humanistic Caring Ability%以人文关怀能力培养为本位的护理专业课教学改革与实践

    Institute of Scientific and Technical Information of China (English)

    贾娟娟; 汪洪杰; 黄弋冰

    2013-01-01

    目的探讨以人文关怀能力培养为本位的护理专业课教学改革的效果。方法采用随机整群抽样,从某校二年级专科护生中抽取8个班作为研究对象,按随机数字表法分为观察组和对照组。对照组在护理专业课教学中遵循传统教学,观察组以人文关怀能力培养为本位对护理专业课的教学内容、教学方法及考核方式进行改革。临床实习中期分别对两组学生及其临床带教老师应用护生人文关怀能力量表和自行设计的调查问卷进行调查,评价实施效果。结果学期结束后护生自评及临床带教老师评价均显示观察组人文关怀能力高于对照组(P<0.05)。结论以人文关怀能力培养为本位的护理专业课教学改革有助于提高护生的人文关怀能力。%Objective To evaluate the effect of teaching reform of nursing specialty courses based on the cultivation of humanistic caring ability. Methods By cluster sampling, eight classes of sophomore nursing students were randomly divided into control group and experiment group. Traditional teaching method was applied in control group while in experiment group, based on the cultivation of humanistic caring ability, a new teaching method including reformed teaching content, teaching method and evaluation mode was used. In mid-term of clinical practice, nursing student humanistic caring ability scale and a self-designed questionnaire were used to evaluate the effect. Results Both nursing students’ self-evaluation and the evaluation on clinical teachers showed students in experiment group presented higher humanistic caring ability (P<0.05). Conclusion Nursing specialty courses based on the cultivation of humanistic caring ability help to enhance nursing students ’ humanistic caring ability.

  7. A numerical method based on probability theory

    Institute of Scientific and Technical Information of China (English)

    唐立; 邹捷中; 杨文胜

    2003-01-01

    By using the connections between Brownian family with drift and elliptic differential equations, an efficient probabilistic computing method is given. This method is applied to a wide-range Diriehlet problem. Detail analysis and deduction of solving the problem are offered. The stochastic representation of the solution to the problem makes a 3-dimensional problem turned into a 2-dimensional problem. And an auxiliary ball is constructed. The strong Markov property and the joint distributions of the time and place of hitting spheres for Brownian family with drift are employed. Finally, good convergence of the numerical solution to the problem over domain with arbitrary boundary is obtained.

  8. Classification and Gradation of Cultivated Land Quality in Bishan County of Chongqing, China

    Institute of Scientific and Technical Information of China (English)

    SHAO Jing'an; GE Xiaofeng; WEI Chaofu; XIE Deti

    2007-01-01

    The conflicts among food security, economic development and ecological protection are the "sticking point"of undeveloped southwestern mountainous areas of China. The objectives of this study are to identify appropriate integrated indicators influencing the classification and gradation of cultivated land quality in the southwestern mountainous area of China based on semi-structure interview, and to promote the monitoring of cultivated land quality in this region.Taking Bishan County of Chongqing as a study case, the integrated indicators involve the productivity, protection, acceptability, and stability of cultivated land. The integrated indicators accord with the characteristics of land resources and human preference in southwestern mountainous area of China. In different agricultural zones, we emphasize different indicators, such as emphasizing productivity, stabilization and acceptability in low hilly and plain agricultural integrative zone (LHP-AIZ), protection, productivity and stability in low mountain and hill agro-forestry ecological zone (LMH-AEZ), and acceptability in plain outskirts integrative agricultural zone (PO-IAZ), respectively. The pronounced difference of classification and gradation of cultivated land, regardless of inter-region or intra-region, is observed, with the reducible rank from PO-IAZ, LHP-AIZ to LMH-AEZ. Research results accord with the characteristics of assets management and intensive utilization of cultivated land resources in the southwestern mountainous area of China.Semi-structure interview adequately presents the principal agent of farmers in agricultural land use and rural land market.This method is very effective and feasible to obtain data of the quality of cultivated land in the southwestern mountainous area of China.

  9. HMM-Based Gene Annotation Methods

    Energy Technology Data Exchange (ETDEWEB)

    Haussler, David; Hughey, Richard; Karplus, Keven

    1999-09-20

    Development of new statistical methods and computational tools to identify genes in human genomic DNA, and to provide clues to their functions by identifying features such as transcription factor binding sites, tissue, specific expression and splicing patterns, and remove homologies at the protein level with genes of known function.

  10. Deforestation and cultivation mobilize mercury from topsoil.

    Science.gov (United States)

    Gamby, Rebecca L; Hammerschmidt, Chad R; Costello, David M; Lamborg, Carl H; Runkle, James R

    2015-11-01

    Terrestrial biomass and soils are a primary global reservoir of mercury (Hg) derived from natural and anthropogenic sources; however, relatively little is known about the fate and stability of Hg in the surface soil reservoir and its susceptibility to change as a result of deforestation and cultivation. In southwest Ohio, we measured Hg concentrations in soils of deciduous old- and new-growth forests, as well as fallow grassland and agricultural soils that had once been forested to examine how, over decadal to century time scales, man-made deforestation and cultivation influence Hg mobility from temperate surface soils. Mercury concentrations in surficial soils were significantly greater in the old-growth than new-growth forest, and both forest soils had greater Hg concentrations than cultivated and fallow fields. Differences in Hg:lead ratios between old-growth forest and agricultural topsoils suggest that about half of the Hg lost from deforested and cultivated Ohio soils may have been volatilized and the other half eroded. The estimated mobilization potential of Hg as a result of deforestation was 4.1 mg m(-2), which was proportional to mobilization potentials measured at multiple locations in the Amazon relative to concentrations in forested surface soils. Based on this relationship and an estimate of the global average of Hg concentrations in forested soils, we approximate that about 550 M mol of Hg has been mobilized globally from soil as a result of deforestation during the past two centuries. This estimate is comparable to, if not greater than, the amount of anthropogenic Hg hypothesized by others to have been sequestered by the soil reservoir since Industrialization. Our results suggest that deforestation and soil cultivation are significant anthropogenic processes that exacerbate Hg mobilization from soil and its cycling in the environment.

  11. Lactose autoinduction with enzymatic glucose release: characterization of the cultivation system in bioreactor.

    Science.gov (United States)

    Mayer, Sonja; Junne, Stefan; Ukkonen, Kaisa; Glazyrina, Julia; Glauche, Florian; Neubauer, Peter; Vasala, Antti

    2014-02-01

    The lactose autoinduction system for recombinant protein production was combined with enzymatic glucose release as a method to provide a constant feed of glucose instead of using glycerol as a carbon substrate. Bioreactor cultivation confirmed that the slow glucose feed does not prevent the induction by lactose. HPLC studies showed that with successful recombinant protein production only a very low amount of lactose was metabolized during glucose-limited fed-batch conditions by the Escherichia coli strain BL21(DE3)pLysS in well-aerated conditions, which are problematic for glycerol-based autoinduction systems. We propose that slow enzymatic glucose feed does not cause a full activation of the lactose operon. However recombinant PDI-A protein (A-domain of human disulfide isomerase) was steadily produced until the end of the cultivation. The results of the cultivations confirmed our earlier observations with shaken cultures showing that lactose autoinduction cultures based on enzymatic glucose feed have good scalability, and that this system can be applied also to bioreactor cultivations.

  12. APPLICATION DIMENSIONAL AND SIMILARITY THEORY IN DETERMINING THE PARAMETERS AND OPERATING MODES OF SOIL CULTIVATING MACHINES

    Directory of Open Access Journals (Sweden)

    Shhirov V. N.

    2015-06-01

    Full Text Available The article presents a study of parameters and modes of operation of machines for soil cultivation. In determining the parameters and modes of operation of machinery for tillage we have applied the theory of similarity and dimensions of physical quantities. We have obtained the regularities of disclosing the relationship of the parameters from the medium to the energy characteristics of the process. As the initial data we used test protocols of machines for soil cultivation (Central - Black Earth, Kubanskaya, Sibirskaya, of North - Caucasion MIS, RosNIITiM : KPI - 3.8, AРC - 3.9, AKV - 4, AKM - 6 - V, AMP - 4 APC - 4 A, AРC - 10 APR - 4.4, APU - 6.5 APSH - 6 , CNC - 6.0, CSТ - 3.8, APC - 4. We defined the formula оf dimension parameters and modes of operation of machines for soil cultivation and properties of soil (traction resistance, depth, width, speed, hardness of the soil, acceleration. Based on dimension theory we have received similarity criteria. Based on the correlation analysis and the least squares method we determined the nature of addiction and the coefficients for it. We have also received a graph for determining the operating modes and parameters of machines for soil cultivation

  13. The Origin of Flooded Rice Cultivation

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    , slow decline of soil fertility, availability of minerals, and resulting in high yield per unit area, which have collectively attained the highly productive cereal cultivation in the warm and humid region. Rice cultivation in marsh is also favorable to raise fish culture, both of which constituted a nutritionally balanced base. Development of irrigation technology to construct flooded farms gave strong bases for stable rice-cultivating society, which in the end formulated the rise of ancient kingdoms of Yue and Wu in China in BC 6th -5th centuries. They were direct descendents of those people who had developed the unique rice cultivation from the era of Hemudu culture, which is dated back to 5 000 BC. Their movement to the south is considered to have established rice-cultivating communities in South China and Southeast Asia, while to the north it transferred the rice-based technology to ancient Korea and Japan and had established there a base for a civilized society.

  14. Enzyme controlled glucose auto-delivery for high cell density cultivations in microplates and shake flasks

    Directory of Open Access Journals (Sweden)

    Casteleijn Marco G

    2008-11-01

    Full Text Available Abstract Background Here we describe a novel cultivation method, called EnBase™, or enzyme-based-substrate-delivery, for the growth of microorganisms in millilitre and sub-millilitre scale which yields 5 to 20 times higher cell densities compared to standard methods. The novel method can be directly applied in microwell plates and shake flasks without any requirements for additional sensors or liquid supply systems. EnBase is therefore readily applicable for many high throughput applications, such as DNA production for genome sequencing, optimisation of protein expression, production of proteins for structural genomics, bioprocess development, and screening of enzyme and metagenomic libraries. Results High cell densities with EnBase are obtained by applying the concept of glucose-limited fed-batch cultivation which is commonly used in industrial processes. The major difference of the novel method is that no external glucose feed is required, but glucose is released into the growth medium by enzymatic degradation of starch. To cope with the high levels of starch necessary for high cell density cultivation, starch is supplied to the growing culture suspension by continuous diffusion from a storage gel. Our results show that the controlled enzyme-based supply of glucose allows a glucose-limited growth to high cell densities of OD600 = 20 to 30 (corresponding to 6 to 9 g l-1 cell dry weight without the external feed of additional compounds in shake flasks and 96-well plates. The final cell density can be further increased by addition of extra nitrogen during the cultivation. Production of a heterologous triosphosphate isomerase in E. coli BL21(DE3 resulted in 10 times higher volumetric product yield and a higher ratio of soluble to insoluble product when compared to the conventional production method. Conclusion The novel EnBase method is robust and simple-to-apply for high cell density cultivation in shake flasks and microwell plates. The

  15. Eucheuma and Kappaphycus : taxonomy and cultivation

    OpenAIRE

    Trono, Jr., C. Gavino

    1992-01-01

    The Genera Eucheuma, Kappaphycus and Hypnea are three important genera of carrageenophytes which are abundant in the Philippines and in the tropical Asia and Western Pacific. The most useful species for the source of kappa carageenan is K. alvarezii called E. "cottonii" of commerce. E. denticulatum called E. "spinosum" of commerce is also the most useful species for the sources of iota carrageenan.The different methods of Eucheuma cultivation were tried in the past from very simple bottom cul...

  16. Isolation and Cultivation of Anaerobes

    DEFF Research Database (Denmark)

    Aragao Börner, Rosa

    2016-01-01

    Anaerobic microorganisms play important roles in different biotechnological processes. Their complex metabolism and special cultivation requirements have led to less isolated representatives in comparison to their aerobic counterparts.In view of that, the isolation and cultivation of anaerobic...... microorganisms is still a promising venture, and conventional methodologies as well as considerations and modifications are presented here. An insight into new methodologies and devices as well as a discussion on future perspectives for the cultivation of anaerobes may open the prospects of the exploitation...

  17. Inculcation Method of Character Education Based on Personality Types Classification in Realizing Indonesia Golden Generation

    Science.gov (United States)

    Sunarto, M. J. Dewiyani; Sagirani, Tri

    2014-01-01

    "The rise of Indonesia Golden Generation" is the theme of National Education Day in 2012. In an effort to create a golden generation; education must be interpreted as a complex problem, in particular the cultivation of character education that was originally using indoctrination method. Given the shifting of the changing times,…

  18. On the Role of Higher Vocational College English Teaching Methods in the Cultivation of Listening and Speaking Abilities%论高职英语教学法对听说能力培养的作用

    Institute of Scientific and Technical Information of China (English)

    李星

    2015-01-01

    The listening and speaking abilities of English lie in the fundamental area, both linguistically and socially;the English education in higher vocational colleges, in terms of its application feature, holds privilege and challenge in the cultivation of listen-ing and speaking abilities. This article, under the orientation of English teaching methods, analyzes the implementation of usual teaching methods in English, so as to search for effective mea-sures to foster listening and speaking skills.%英语听说能力在语言层面和社会层面都处于基础地位,高职英语教学就其应用性而言对于听说能力的培养既有优势也有挑战。本文以英语教学法为导向,分析高职英语常用教学方法的实施,从中探寻有效的听说能力培养措施。

  19. Scope-Based Method Cache Analysis

    DEFF Research Database (Denmark)

    Huber, Benedikt; Hepp, Stefan; Schoeberl, Martin

    2014-01-01

    , as it requests memory transfers at well-defined instructions only. In this article, we present a new cache analysis framework that generalizes and improves work on cache persistence analysis. The analysis demonstrates that a global view on the cache behavior permits the precise analyses of caches which are hard......The quest for time-predictable systems has led to the exploration of new hardware architectures that simplify analysis and reasoning in the temporal domain, while still providing competitive performance. For the instruction memory, the method cache is a conceptually attractive solution...

  20. Triptycene-based dianhydrides, polyimides, methods of making each, and methods of use

    KAUST Repository

    Ghanem, Bader

    2015-12-30

    A triptycene-based monomer, a method of making a triptycene-based monomer, a triptycene-based aromatic polyimide, a method of making a triptycene- based aromatic polyimide, methods of using triptycene-based aromatic polyimides, structures incorporating triptycene-based aromatic polyimides, and methods of gas separation are provided. Embodiments of the triptycene-based monomers and triptycene-based aromatic polyimides have high permeabilities and excellent selectivities. Embodiments of the triptycene-based aromatic polyimides have one or more of the following characteristics: intrinsic microporosity, good thermal stability, and enhanced solubility. In an exemplary embodiment, the triptycene-based aromatic polyimides are microporous and have a high BET surface area. In an exemplary embodiment, the triptycene-based aromatic polyimides can be used to form a gas separation membrane.

  1. Proposal for Scrambled Method based on NTRU

    Directory of Open Access Journals (Sweden)

    Ahmed Tariq Sadiq

    2015-08-01

    Full Text Available Scrambling is widely used to protect the security of data files such as text, image, video or audio files; however, it is not the most efficient method to protect the security of the data files. This article uses NTRU public key cryptosystem to increase the robustness of scrambling of sound files. In this work, we convert the sound file into text, and then scramble it in the following way: first, we encrypt the header of the sound file then, scramble the data of the file after the header in three stages. In each stage we scramble the data of the sound file and keep the original order of data in an array then, the three arrays are encrypted by the sender and sent with the encrypted header to the receiver in one file, while the scrambled data of the sound file is sent to the receiver in another file. We have tested the proposed method on several sound files; the results show that the time of encryption and decryption is reduced to approximately one-third, or less, compared to encrypting the file using NTRU.

  2. [Culture based diagnostic methods for tuberculosis].

    Science.gov (United States)

    Baylan, Orhan

    2005-01-01

    Culture methods providing isolates for identification and drug susceptibility testing, still represent the gold standard for the definitive diagnosis of tuberculosis, although the delay in obtaining results still remains a problem. Traditional solid media are recommended for use along with liquid media in primary isolation of mycobacteria. At present, a number of elaborate culture systems are available commercially. They range from simple bottles and tubes such as MGIT (BD Diagnostic Systems, USA), Septi-Chek AFB (BD, USA) and MB Redox (Biotest Diagnostics, USA) to semiautomated system (BACTEC 460TB, BD, USA) and fully automated systems (BACTEC 9000 MB [BD, USA], BACTEC MGIT 960 [BD, USA], ESP Culture System II [Trek Diagnostics, USA], MB/BacT ALERT 3D System [BioMérieux, NC], TK Culture System [Salubris Inc, Turkey]). Culture methods available today are sufficient to permit laboratories to develop an algoritm that is optimal for patients and administrative needs. In this review article, the culture systems used for the diagnosis of tuberculosis, their mechanisms, advantages and disadvantages have been discussed under the light of recent literature.

  3. Pressure on Cultivated Land in Fengxian County,Jiangsu Province,China

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Based on the introduction of the general situation of research region,pressure index model of cultivated land is adopted according to the data of population,cultivated land area,crop sowing area,grain sowing area,and unit grain yield in the Statistical Yearbook of Fengxian County.The change of relevant factors of cultivated land pressure index over time is analyzed,as well as the tension level of cultivated land resources.Research shows that the pressure on cultivated land is relatively great in Fengxian County in the years 1999-2005.Grain supply and demand is still under the unsafe state.There will be more prominent contradiction between supply and demand of cultivated land in Fengxian County in the future,and the grain security will under greater pressure.Several countermeasures are put forward to ease the pressure on cultivated land in Fengxian County,in order to realize the sustainable use of cultivated land resources,to ensure the supply and demand balance of grain and cultivated land,and to stabilize and improve the grain productivity in Fengxian County,such as protecting the current cultivated land resources by the strictest protection system of cultivated land,enhancing the intensity of land development and reclamation,enlarging the area of cultivated land,increasing the agricultural sci-tech input,and improving the grain unit yield.

  4. GALERKIN MESHLESS METHODS BASED ON PARTITION OF UNITY QUADRATURE

    Institute of Scientific and Technical Information of China (English)

    ZENG Qing-hong; LU De-tang

    2005-01-01

    Numerical quadrature is an important ingredient of Galerkin meshless methods. A new numerical quadrature technique, partition of unity quadrature (PUQ),for Galerkin meshless methods was presented. The technique is based on finite covering and partition of unity. There is no need to decompose the physical domain into small cell. It possesses remarkable integration accuracy. Using Element-free Galerkin methods as example, Galerkin meshless methods based on PUQ were studied in detail. Meshing is always not required in the procedure of constitution of approximate function or numerical quadrature, so Galerkin meshless methods based on PUQ are "truly"meshless methods.

  5. Algebraic Verification Method for SEREs Properties via Groebner Bases Approaches

    Directory of Open Access Journals (Sweden)

    Ning Zhou

    2013-01-01

    Full Text Available This work presents an efficient solution using computer algebra system to perform linear temporal properties verification for synchronous digital systems. The method is essentially based on both Groebner bases approaches and symbolic simulation. A mechanism for constructing canonical polynomial set based symbolic representations for both circuit descriptions and assertions is studied. We then present a complete checking algorithm framework based on these algebraic representations by using Groebner bases. The computational experience result in this work shows that the algebraic approach is a quite competitive checking method and will be a useful supplement to the existent verification methods based on simulation.

  6. Beam Parameters Measurement Based On Tv Methods

    CERN Document Server

    Klimenkov, E; Milichenko, Yu; Voevodin, V

    2004-01-01

    The paper describes hardware and software used to control TV-cameras and to process TV-images of luminescent screens placed along the beam transfer lines. Industrial devices manually control the movements and focusing of the cameras. All devices are linked to PC via PCI interfaces with homemade drivers for Linux OS and provide both selection of camera and digitizing of video signal synchronized with beam. One part of software provides means to set initial parameters using PC consol. Thus an operator can choose contrast, brightness, some number of significant points on TV-image to calculate beam position and its size. Second part supports remote TV controls and data processing from Control Rooms of U-70 complex using set initial parameters. First experience and results of the method realization are discussed.

  7. Adaptive Mixture Methods Based on Bregman Divergences

    CERN Document Server

    Donmez, Mehmet A; Kozat, Suleyman S

    2012-01-01

    We investigate adaptive mixture methods that linearly combine outputs of $m$ constituent filters running in parallel to model a desired signal. We use "Bregman divergences" and obtain certain multiplicative updates to train the linear combination weights under an affine constraint or without any constraints. We use unnormalized relative entropy and relative entropy to define two different Bregman divergences that produce an unnormalized exponentiated gradient update and a normalized exponentiated gradient update on the mixture weights, respectively. We then carry out the mean and the mean-square transient analysis of these adaptive algorithms when they are used to combine outputs of $m$ constituent filters. We illustrate the accuracy of our results and demonstrate the effectiveness of these updates for sparse mixture systems.

  8. Economic Efficiency of Selected Crops Cultivated under Different Technology of Soil Tillage

    Directory of Open Access Journals (Sweden)

    Vach M.

    2016-03-01

    Full Text Available The objective of this study was the model comparison and economic evaluation of different methods of soil tillage and crop stand establishments used. Based on yield results (winter wheat, spring barley, and white mustard cultivated in three-crop rotation from field experiments with conventional, conservation with minimum tillage, and no-tillage methods conducted at the site Prague-Ruzyně, model economic balances were evaluated. Prices of the main products were determined based on the yield results from the period 2010-2013 and the current market prices. In the individual tillage systems, the total costs of production of evaluated crops were counted up and profitability was calculated as a ratio of profit to total costs. The highest total costs of crop cultivation were identified in cereals under conventional soil tillage, on the contrary, the lowest in cereals cultivated under conservation tillage technology. As for the growing technologies, the highest profitability was found in winter wheat, as for the tillage methods, it was in the conservation variant with minimum tillage. The economic evaluation for individual crops was based on standards of growing technologies and particular work operations.

  9. 毛囊细胞高效分离培养方法的建立%Establishment of High Performance Method for Isolation and Cultivation of Hair Follicle Stem Cells of Neonatal Rats

    Institute of Scientific and Technical Information of China (English)

    陈杏晔; 李凡; 刘爱军

    2014-01-01

    Objective To establish a simple , practical , highly-effective and stable method for the isolation and cultivation of rat hair follicle cells. Methods Under sterile condition, single hair follicle was taken out after the skin around the barbel of SD neonatal rats was sheared off. And then the hair follicles were digested with two-step method with Type Ⅰcollagenase and trypsin. The obtained cell suspension was planted into the culture plate which was covered with extracellular matrix, and then was cultivated with K-SFM culture medium containing fetal bovine serum with volume fraction of 1%. On the next day, K-SFM culture medium was replaced with serum-free culture medium. The remaining tissues were cut into pieces and spread out in the culture flask, and then were cultivated with HG-DMEM culture medium containing serum. Two kinds of cells were harvested and then were identified by immunofluorescence. The hair follicle epithelial cells were tested by flow cytometer. Results The hair follicle epithelial cells obtained through the above methods showed rapid adherence, and were round or polygon-like , with typical cobblestone-like morphology. The long spindle-shaped cells were seen around the tissues cultivated, having many protrusions on the surface of the cells, and they were interconnected into reticular structure. The expression of cytokeratin 15, cytokeratin 19 and β1 integrin in epithelial cells were positive. Most of the epithelial cells were in the G1 phase, accounting for 75.6%. The expression of laminin ( LN), fibronectin ( FN) and vimentin in the connective tissue sheath cells were also positive. Conclusion The cells harvested by modified two-step enzyme digestion method have confirmed as hair follicle cells and fibroblasts, and the obtained cells are of rapid adherence, good homogeneity, and active proliferation.%【目的】建立一种简单易行、高效稳定、细胞活性好的毛囊细胞分离培养方法。【方法】无菌条件下剪下SD乳

  10. The use of 32P Method to Evaluate the Growth of Lowland Rice Cultivated in a System of Rice Intensification (SRI

    Directory of Open Access Journals (Sweden)

    A. Citraresmini

    2013-08-01

    Full Text Available A pot experiment has been conducted to evaluate the growth of the Dyah Suci, a lowland rice variety, in an SRI (System of Rice Intensification planting system. The phosphorus-32 (32P isotope technique was used to evaluate the growth of plants in relation with their phosphorus uptake. The uptake was assumed to vary in the same direction as the growth of the plant. The 32P uptake is assumed to vary in the opposite direction to the plant’s total phosphorus uptake. Here the 32P uptake is expressed in count per minutes (cpm which is then transformed to disintegration per minute (dpm. The results show that, in terms of promoting the plant’s uptake of phosphorus, the SRI planting system is superior to the conventional planting system, and it is manifested in the higher dry weight of straw and grain. From this experiment it is concluded that the 32P method can be used satisfactorily as a tool for explaining the relation between P-uptake and plant growth

  11. 兔骨膜细胞分离培养的方法改进%A modified method forin vitroisolation and cultivation of periosteal cells in rabbits

    Institute of Scientific and Technical Information of China (English)

    张峻玮; 陆海涛; 袁峰; 杨宇明

    2016-01-01

    BACKGROUND:Periosteum is considered as a source of seed cels for cel therapydue toits biological features. OBJECTIVE:To seek the optimal way to isolate and culture rabbit periosteal cels and identify their biological features. METHODS:Rabbit periosteum on facies medialis tibiae was taken out under aseptic conditions. Periosteal cels isolated through the digestion of type II colagenase with the explants culture method were cultured in DMEM/F12 complete medium. Cel ultrastructure was observedunderan inverted microscope. Periosteal cel proliferation was determined bycel counting kit-8assay. Cel surface antigensCD90 and CD105 were determined using flow cytometry. Osteogenic andlipogenic induction mediums were applied to induce periosteal cels to differentiate into osteocytes and adipocytes, respectively. After 2 weeks of induction, cels were harvestedfor alizarin red staining and oil red O staining to assay the calciumnodules and lipid droplet. RESULTS AND CONCLUSION:The digestion of type II colagenase with the explants culture method shortened the period of primary cels culture and enhanced the survival rate, which causedhigher purity and stronger reproductive activity of harvested periosteal cels. Primary cultured periosteal cels grew in form of spindle spiral or paralel. Alizarin red andOil red O staining verified the multi-directional differentiation potentiality of periosteal cels. These findings suggest that the periosteal cels with high purity,strong reproductive activity,andmulti-directional differentiation potentialitycanbe harvested in short time using digestion of type II colagenase with the explants culture method.%背景:近年来,骨膜作为细胞治疗的种子细胞来源,因其自身的优越性而备受关注。目的:探讨兔骨膜细胞分离培养的最佳方法及其生物学特性。方法:无菌条件下取出兔胫骨内侧面骨膜,以Ⅱ型胶原酶消化结合组织块贴壁法分离培养兔骨膜细胞,置于DMEM/F12完全

  12. Concentrations of some macro and micro plant nutrient of cultivated soils in Central and Eastern Blacksea Region and their mapping by inverse distance weighted (IDW method

    Directory of Open Access Journals (Sweden)

    Mehmet Arif Özyazıcı

    2015-11-01

    Full Text Available The aim of this study was to determine plant nutrients content and to in terms of soil variables their soil database and generate maps of their distribution on agricultural land in Central and Eastern Black Sea Region using geographical information system (GIS. In this research, total 3400 soil samples (0-20 cm depth were taken at 2.5 x 2.5 km grid points representing agricultural soils. Total nitrogen, extractable calcium, magnesium, sodium, boron, iron, copper, zinc and manganese contents were analysed in collected soil samples. Analysis results of these samples were classified and evaluated for deficiency, sufficiency or excess with respect to plant nutrients. Afterwards, in terms of GIS, a soil database and maps for current status of the study area were created by using inverse distance weighted (IDW interpolation method. According to this research results, it was determined sufficient plant nutrient elements in terms of total nitrogen, extractable iron, copper and manganese in arable soils of Central and Eastern Blacksea Region while, extractable calcium, magnesium, sodium were found good and moderate level in 66.88%, 81.44% and 64.56% of total soil samples, respectively. In addition, insufficient boron and zinc concentration were found in 34.35% and 51.36% of soil samples, respectively.

  13. Study on cultivation objectives and curriculum system of nursing postgraduate constructed by Delphi method%Delphi法构建护理学研究生培养目标及课程体系的研究

    Institute of Scientific and Technical Information of China (English)

    蒋小平; 颜莉; 郑显兰; 刘贤; 魏晓琼

    2015-01-01

    Objective:To construct the specific ,detailed and operable cultivation objectives of nursing master postgraduates and curriculum system in favor of the cultivation of core competency of nursing professional mas‐ter ,so as to provide the references for the training program of medical colleges and higher nursing education management .Methods:By investigating the related literatures of training scheme of nursing academic type mas‐ter postgraduates in Chinese and English database various colleges and universities public web sites in nearly 5 years in 31 domestic and foreign universities ,and combining with Taylor’s education target model of theory frame ,Delphi specialist letter questionnaire was drafted ,a total of 2 rounds of inquiry were carried out .Statisti‐cal analysis was performed for the positive coefficient ,authoritative coefficient of expert ,expert opinion coordi‐nation degree and the mean value of indexes at all levels ,and coefficient of variation ,and to analyze the practica‐bility and significance of all indexes .Results:A total of 26 experts participated in inquiry ,value of their authority degree was 0 .88 ,and Kendall coordinated coefficient was 0 .531 ,χ2 test showed P<0 .01 ;the cultivation objec‐tives formed through two rounds of expert consultation had 9 concrete items ,including knowledge ,skills and humanistic accomplishment ;the formed curriculum system included 30 subjects totally ,in which there were 4 public obligatory courses ,6 professional basic courses ,20 professional expand option courses .Conclusion:The construction of nursing postgraduate cultivation objectives and curriculum system formed in this study were more specific and operable ,can provide references for development of postgraduate cultivation scheme of nursing profession .%[目的]构建具体、细化及可操作的护理学硕士研究生培养目标和有利于护理专业硕士生核心能力培养的课程体系,为各医学院校和高等护理教

  14. Cultivation of microalgae in industrial wastewaters

    DEFF Research Database (Denmark)

    van Wagenen, Jonathan Myerson

    cultivation. When algae are grown within a wastewater treatment plant, the use of the chemical energy stored in the organic carbon dissolved in the wastewater could also be a useful option. Conventional aerobic sewage treatment expends much energy in breaking down the biomass to CO2. However, various......Microalgae production for the purpose of clearing wastewater has been researched for at least half a century. Such systems have a dual benefit: first, they prevent nutrients from entering water bodies and causing eutrophication; second, they transform sunlight and carbon dioxide into a biomass...... that has many potential uses. Unfortunately, the current high costs of cultivation have limited the development and exploitation of such systems, resulting in only a few full-scale algae wastewater treatment installations and a small industry based mostly around food and pigments. This thesis contributes...

  15. 高校科研工作者职业伦理培育--基于道德想象力路径%Cultivation of University Researchers′Professional Ethics:Based on Moral Imagination

    Institute of Scientific and Technical Information of China (English)

    林曼峰

    2015-01-01

    Abilities and of unversity researchers’moral imagination are usually higher than common social groups, and professional ethics can be cultivated by strengthening the moral imagination. Moral imagination can help researchers improve the moral cognition,process value conflicts effectively,reduce risk behavior research,and enhance the sense of social responsibility. University researchers can cultivate moral imagination through empathy,speech,discipline,prac-tice and other methods.%科研工作者道德想象能力和条件高于一般社会群体,运用道德想象力培育职业伦理可行性强。道德想象力能够帮助科研工作者提高道德认知能力,有效因应价值冲突,降低科研行为风险,增强社会责任意识。高校科研工作者道德想象力的训练路径包括移情法、言说法、惩戒法、实践法等。

  16. 基于职业能力培养的市场营销课程教学改革%Research on Teaching Reform in Marketing Course Based on Vocational Competency Cultivation

    Institute of Scientific and Technical Information of China (English)

    崔玉华

    2012-01-01

    职业能力培养是市场营销课程适应市场、服务企业、创新人才培养模式的需要。从市场营销课程内容的改革、教学方法的改革、考试的改革以及教学中应当把握的原则4个方面,提出了市场营销课程改革必须从知识传授型向职业技能型转变,从课堂为中心向社会实践为中心转变,变教师为教练,变封闭学习为开放学习的建议。%Vocational competency cultivation is the needs of marketing course adapting to the market, serving enter-prises, and innovating talent cultivation model. This paper proposes that teaching reform in marketing course should be from knowledge initiating to vocational skills, from classroom - centered to practice - centered, from teachers to coaches, from closed -end learning to open learning through the research on the reform of course content, the re- form of teaching method, the reform of examination and the teaching principles.

  17. A Study and Practice of College Students' Emotional Quotient Cultivation Based on Quality Development%基于素质拓展平台大学生情商培养研究与实践

    Institute of Scientific and Technical Information of China (English)

    嵇敏; 杨丹; 李冠华; 张玲彬

    2015-01-01

    基于素质拓展平台新模式运作下大学生情商培养研究与实践,通过在河海大学大禹学院2013级本科生中开展野外素质拓展,寒暑假感恩教育,社会实践、公益劳动等素质拓展学分的系列活动,在实践的强化培养下,增强学生的团结、爱心、宽容、感恩、自信等情商特性,以促进学生更好地认识自我、管理自我、激励自我。%This paper is a study and practice of college students' Emotional Quotient cultivation based on a new model which is quality development. In Grade 2013 undergraduates in Dayu College of Hohai University, field quality development, thanks-giving education during summer and winter vacation, social prac-tice, welfare work and other activities of quality development credits were held. Under the practice of intensive cultivation, students' non-intelligence characteristics of EQ, such as solidar-ity, love, tolerance, thanksgiving and self-confidence, , were strengthened, and they could understand, manage themselves, and motivate themselves better.

  18. 基于大学校园环境建设的创新人才培养研究--生态系统的视角%The Cultivation of Innovative Talents based on Campus Environment Constructionfrom the Perspective of Ecosystem

    Institute of Scientific and Technical Information of China (English)

    吕欢; 杨娟娟; 周鹏生; 马德山

    2015-01-01

    From the perspective of ecosystem , this article explains the basic ideas of social ecology , and the ecosystem view of innovative talents cultivation based on university environmental education con -struction , which means the best fitment of establishment between individual and environment .Then the article also puts forward some ideas on how to cultivate innovative talents .Finally , this paper makes some case-analyses of implement of innovation curriculum directed by ecosystem .%本文从生态系统的视角,阐述了社会生态的基本观点,论述了基于大学校园环境建设的创新人才培养生态系统观,即建立个人与环境的最佳适配性,并从生态系统视角就创新人才培养途径提出了思考。以生态系统为引领,对创新课堂的实施进行了案例分析。

  19. 基于改进的SVM算法的耕地地力评价模型研究%Model of Productivity of Cultivated Land Based on Improved-SVM

    Institute of Scientific and Technical Information of China (English)

    李露璐

    2012-01-01

    为了提高农业管理水平,将计算机智能技术与农业技术相结合,提出基于改进的SVM算法建立标准农田地力等级的评价模型,在评价模型中利用频繁闭集挖掘算法获取特征向量集合,再利用SVM算法建立耕地地力评价模型.仿真结果表明:评价结果符合当地实际情况,并且与传统的评价模型相比,该模型对非线性特征值评价评价中精确度更高.%In order to improve the level of management of agriculture and combined with artificial intelligence, a standard evaluation on soil fertility grade for cultivated land was made based on improved-SVM. This model used data mining algorithm of FCIs to obtain the list of feature vectors, then the evaluation model of soil fertility grade for cultivated land was established by SVM algorithm. The experiment result was basically consistent with the actual status, status, showed that the model is better practiocable and higher accuracy for evaluation of the nonlinear characters.

  20. 基于供应链管理模式的军事物流人才培养研究%Study on Cultivation of Military Logistics Talent Based on Supply Chain Management Mode

    Institute of Scientific and Technical Information of China (English)

    王平; 刘晟; 肖侃

    2012-01-01

    随着军事物流领域中高新技术的不断发展,培养高层次、高素质.富有革新精神的军事物流人才已成为我军物流现代化发展的关键.在分析军事物流人才培养现状的基础上,着重探讨了基于供应链管理模式的军事物流人才培养的特点,构建了军事物流人才培养的供应链模式,并讨论了相应的优化措施,着力探求满足部队需求的军事物流专业教学模式,重点解决理论和实际结合问题.%In this paper, we mainly discussed the characteristics of the cultivation of military logistics talents based on the supply chain management mode, established the supply chain mode of military logistics talent cultivation and discussed the corresponding optimization measures.

  1. On cultivating of business English talents in applied universities based on social demand%基于社会需求视角的应用型高校商务英语人才培养研究

    Institute of Scientific and Technical Information of China (English)

    周秀英; 谢金艳

    2013-01-01

    Business English majors cannot produce compound Business English talents if insisting on the traditional cultivating mode of English knowledge plus business knowledge. This paper, based on current social demand for compound talents, intends to investigate a more practical cultivation mode for Business English majors, so as to provide a theoretical reference for applied universities.%应用型高校商务英语专业,如果仅限于“英语+商务”简单的知识累加式培养模式,则无法培养出高质量的复合型人才。本文从社会对人才的实际需求视角出发,探讨有效的可操作性的应用型商务技能人才培养模式,旨在为应用型高校商务英语专业课程体系设置,及专业人才培养目标制定提供理论化借鉴。

  2. Antimicrobial activity and mineral composition of shiitake mushrooms cultivated on agricultural waste

    Directory of Open Access Journals (Sweden)

    Kérley Braga Pereira Bento Casaril

    2011-10-01

    Full Text Available The antimicrobial activity and mineral composition of shiitake mushrooms were evaluated in four isolates of Lentinula edodes. Mushrooms were cultivated on artificial logs, based on eucalyptus sawdust enriched with 20% rice, wheat, or soybean bran, or combination of 10% of two of these supplements. The substrates were humidified with a 0.1% mate tea extract or water. Logs of Eucalyptus grandis were also used to cultivate the shiitake mushrooms. The antimicrobial activity of an aqueous extract, corresponding to 40 mg of mushroom dry matter, was in some cases, depending on the isolate, able to inhibit both Bacillus subtilis and Escherichia coli K-12, independent of substrate composition or the growth stage of the mushrooms. Nitrogen, phosphorus, potassium, magnesium and calcium concentrations varied according to the substrate on which the mushrooms were cultivated, being, generally, higher with cultivation on artificial rather than natural eucalyptus logs. It could be concluded that, in addition to the fungal isolate, substrate composition and, processing methods must be considered during the production of antimicrobial substance(s as well as in the mushroom nutritional composition.

  3. Utilization of industrial dairy waste as microalgae cultivation medium : a potential study for sustainable energy resources

    Science.gov (United States)

    Nurmayani, S.; Sugiarti, Y.; Putra, R. H.

    2016-04-01

    Microalgae is one of biodiesel resources and call as third generation biofuel. Biodiesel is one alternative energy that being developed. So study about resource of biodiesel need a development, for the example is development the basic material such as microalgae. In this paper we explain the potential use of dairy waste from industry as a cultivation medium of microalgae for biodiesel production. Dairy waste from dairy industry contains 34.98% protein, 4.42% lactose, 9.77% fiber, 11.04% fat, 2.33% calcium, 1.05% phosfor, and 0.4 % magnesium, meaning that the dairy waste from dairy industry has a relatively high nutrient content and complete from a source of carbon, nitrogen and phosphorus as macro nutrients. The method in this paper is literature review to resulting a new conclusion about the potency of waste water from dairy industry as microalgae cultivation medium. Based on the study, the dairy waste from dairy industry has potency to be used as cultivation medium of Botryococcus braunii in the production of biodiesel, replacing the conventional cultivation medium.

  4. Evaluation on Cultivated Land Resource Ecologi-cal Service Value in Wuhan Metropolitan Area

    Institute of Scientific and Technical Information of China (English)

    Jinhua TANG; Yinghui Ll; Gangqiang CHEN; Lei YU; Sujuan Ll

    2014-01-01

    The land ecosystem service function value contains the gas regulating function value, air purification function value, water conservation value, land conser-vation value, soil nutrient conservation value and maintain biodiversity value. Based on the method of market value and the data of Hubei Statistical yearbook, cultivat-ed land resources of ecosystem service function value is 730 038.69Mil ion in 2007-2009, and 714 774.99 Mil ion in 2010-2012 in Wuhan Metropolitan Area. After three years of development, Wuhan Metropolitan Area of ecological value of cultivated land has increased by about 3.97%. From the point of each composition, the im-portance of gas regulating function of cultivated land resources and water conserva-tion function is the forefront. From the point of spatial distribution, the highest of annual average value of the cultivated land ecological was Huanggang (1.725 bil ion yuan), the lowest was Ezhou (217.868 6 mil ion yuan) in 2010-2012. From the point of space changes, the land ecological value increased only in Qianjiang city, while farmland ecological value in other cities was fal ing The conclusion is of direc-tive significance to the regional land protection policy and the farmland reasonable distribution, for Wuhan Metropolitan Area and other areas.

  5. A study on Heavy Metal Contents in Cultivated Wild Ginseng from Korea and China

    Directory of Open Access Journals (Sweden)

    Jong-Yoon,Kim

    2007-02-01

    Full Text Available Objectives : Heavy metal contents in cultivated wild ginseng from Korea and China were evaluated for the safety purpose. Methods : Arsenic(As, cadmium(Cd, lead(Pb, mercury(Hg, copper(Cu, chromium(Cr, and selenium(Se from the root, stem, and leaves of the Korean cultivated wild ginseng and the root of the Chinese cultivated wild ginseng were analyzed for the existence of heavy metal contents. Results : Most of heavy metals weren't detected in all the samples. Copper and lead were found in very minute volume and didn't reach the toxic concentration level of 10mg/kg. Samples used in the test were grown in non-contaminated areas, free from heavy metal contamination. Conclusions : Based on above results, cultivated wild ginseng from Korea and China are relatively safe from the heavy metal exposure. But for more rigorous quality control, an epidemiological survey using the samples from more diverse areas should be conducted.

  6. Effects of Urbanization-Induced Cultivated Land Loss on Ecosystem Services in the North China Plain

    Directory of Open Access Journals (Sweden)

    Wei Song

    2015-06-01

    Full Text Available Since the implementation of market oriented economic reform in 1978, China has been on the track of rapid urbanization. The unprecedented urbanization in China has resulted in substantial cultivated land loss and rapid expansion of urban areas. The cultivated land loss due to urbanization not only threatens food security in China, but has also led to ecological system degradation to which close attention should be paid. Therefore, we examined the effects of the conversion from cultivated to urban areas on the ecosystem service in the North China Plain on the basis of a net primary productivity based ecosystem service model (NESM and a buffer comparison method. Cultivated land loss due to urbanization in the North China Plain led to a total loss of ecosystem service value of 34.66% during the period 1988–2008. Urban expansion significantly decreased the ecosystem service function of water conservation (–124.03%, nutrient cycling (–31.91%, gas regulation (−7.18%, and organic production (–7.18%, while it improved the soil conservation function (2.40%. Land use change accounted for 57.40% of the changes in ecosystem service and had a major influence on the changes in nutrient cycling and water conservation. However, climate change mainly determined the changes in gas regulation, organic production, and soil conservation.

  7. 基于创新能力培养的市场营销学课程教学改革探索%Marketing Course Teaching Reform Based on the Cultivation of Innovation Ability

    Institute of Scientific and Technical Information of China (English)

    付存军

    2012-01-01

      The nature of the marketing demands innovation,the drastic changes in the social and economic environment requires that Marketers must innovatively apply the marketing theory to solve the new problems emerging ceaselessly, so the marketing course teaching should focus on the cultivation of students' innovative ability. To achieve this goal, first, the cultivation of students' innovative ability should be embodied in the curriculum teaching target planning, secondly, the marketing curriculum content should properly adjusted, thirdly, it should be flexibly applied in the teaching process of questioning teaching method, scene teaching method, case teaching method and practice teaching method, finally it should be reflected the cultivation of students' innovative ability in course evaluation%  市场营销学的本质要求创新,急剧变化的社会经济环境要求市场营销者必须具备创新性运用市场营销理论解决不断出现的新问题的能力,因而市场营销学课程教学应注重对学生创新能力的培养。为实现该目标,首先应将学生创新能力的培养要求体现在课程教学培养目标规划中,其次应对市场营销学课程内容进行恰当的调整,再次应该在课程教学过程中灵活运用设疑式教学法、情景式教学法、案例教学法及实践教学法等方式方法,最后在课程考核中应该体现出对学生创新能力培养的重视。

  8. AN IMAGE RETRIEVAL METHOD BASED ON SPATIAL DISTRIBUTION OF COLOR

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Color histogram is now widely used in image retrieval. Color histogram-based image retrieval methods are simple and efficient but without considering the spatial distribution information of the color. To overcome the shortcoming of conventional color histogram-based image retrieval methods, an image retrieval method based on Radon Transform (RT) is proposed. In order to reduce the computational complexity,wavelet decomposition is used to compress image data. Firstly, images are decomposed by Mallat algorithm.The low-frequency components are then projected by RT to generate the spatial color feature. Finally the moment feature matrices which are saved along with original images are obtained. Experimental results show that the RT based retrieval is more accurate and efficient than traditional color histogram-based method in case that there are obvious objects in images. Further more, RT based retrieval runs significantly faster than the traditional color histogram methods.

  9. Utilization zoning of cultivated land based on net primary productivity in Guanzhong-Tianshui Economic Region%基于净初级生产力的关中-天水经济区耕地利用分区研究

    Institute of Scientific and Technical Information of China (English)

    杨海娟; 温晓金; 刘焱序

    2013-01-01

    As a populous nation, improve grain production capacity along with rational use and protection of cultivated land resources has posed a considerable challenge in domestic agriculture and land related research in China. Higher NPP for cultivated lands has suggested the existence of more organic biomass. This has been critical for the final production of food crops in the country. It was therefore likely for research on NPP to provide the basis for resolving food security issues. Functional zoning has been the commonly used method to guarantee sustainable use of land. Presently, however, heavily fragmented research merely described real supply of cultivated lands. A deeper understand on the potential reserves of cultivated lands was needed in this regard. Based on remote sensing observation, it is possible to have statistics of the output of a large number of cultivated lands within a short time. Compared with the yearbook data, remote sensing observation has advantages including timeliness and spatial precision. Remote sensing observations have therefore been strongly supplemental to statistical data. NPP estimated by remote sensing was used as crop biomass in cultivated lands instead of the traditional calculations based statistics data. Cultivated land in the Guanzhong-Tianshui Economic Region (GTER) was zoned by using neural network algorithm model and remote sensing data in 2001-2009 substituting for statistic crop yield data. Then the wavelet neural network was used to predict the NPP in the zoned regions. Three results were eventually attained. 1) From 2002 to 2009, total estimated NPP per year in GTER was 1.6×107 t. It showed large variation patterns between estimated NPP data and statistics grain data for cultivated lands in GTER. This suggested statistical and remote sensing data were not substitutable for one another. As clustering function was unknown, zoning via estimated NPP data reflected a more universal adaptability than via statistical data. 2) The

  10. Bionic optimization research of soil cultivating component design

    Institute of Scientific and Technical Information of China (English)

    GUO ZhiJun; ZHOU ZhiLi; ZHANG Yi; LI ZhongLi

    2009-01-01

    The basic biomechanical laws that apply to the clawed toes of animals with powerful digging abilities and the optimal bionic design of curved soil cultivating components with an analogous contour were researched in a novel way. First, the curvature and profile of the inside contour line of a field mouse's clawed toe were analyzed. The finite element method (FEM) was then used to simulate the working process in order to study the changing characteristics of the working resistance of bionic soil-engaging surfaces and the stress field of the processed soil. A straight-line cultivating component was used for comparative analysis. In accordance with the simulation results, a series of soil cultivating components of varying design were manufactured. An indoor soil bin experiment was carried out to measure their working resistance and validate the results of the FEM analysis. The results of this research would have important values in the optimization design of cultivating components for energy and cost savings.

  11. Nitrous oxide emissions from rapeseed cultivation in Germany

    Science.gov (United States)

    Fuß, Roland; Andres, Monique; Hegewald, Hannes; Kesenheimer, Katharina; Koebke, Sarah; Räbiger, Thomas; Suárez Quiñones, Teresa; Walter, Katja; Stichnothe, Heinz; Flessa, Heinz

    2016-04-01

    About 12 % of Germany's agricultural area is used for rapeseed cultivation and two third of the harvest is converted to biodiesel. Due to requirements of the EU Renewables Directive the greenhouse gas (GHG) balance of rapeseed cultivation must be reported and sustainability criteria and GHG savings compared to fossil fuel must be achieved and certified. Current certified methodology estimates N2O field emissions from rapeseed cultivation using the IPCC Tier 1 approach based on a global emission factor (N2O emission per unit nitrogen fertilizer input) of 1 %, which is not specific for the crop. We present results from three years of measurements (2013 - 2015) on five field trials in Germany, which combined with data from a meta-analysis suggest that GHG emission factors of German rapeseed cultivation are lower than thought previously. Furthermore, results suggest that substitution of mineral fertilizers with organic fertilizers is a valid mitigation option since it avoids GHG emissions during production of mineral fertilizers.

  12. Systems and methods for interpolation-based dynamic programming

    KAUST Repository

    Rockwood, Alyn

    2013-01-03

    Embodiments of systems and methods for interpolation-based dynamic programming. In one embodiment, the method includes receiving an object function and a set of constraints associated with the objective function. The method may also include identifying a solution on the objective function corresponding to intersections of the constraints. Additionally, the method may include generating an interpolated surface that is in constant contact with the solution. The method may also include generating a vector field in response to the interpolated surface.

  13. Subpixel edge detection method based on low-frequency filtering

    Science.gov (United States)

    Bylinsky, Yosip Y.; Kotyra, Andrzej; Gromaszek, Konrad; Iskakova, Aigul

    2016-09-01

    A method of edge detection in images is proposed basing that based on low-frequency filtering. The method uses polynomial interpolation to determine the coordinates of the edge point with subpixel accuracy. Some experiments have been results also have been provided.

  14. 基于形象思维的创新能力培养的分析%Analysis of Cultivation for Innovation Ability Based on Thinking in Images

    Institute of Scientific and Technical Information of China (English)

    赵晓运

    2012-01-01

    分析了创新教育中形象思维的独特性、基础性和源头性,指出创新能力是一种社会属性,可以通过锻炼和培养而得到提高.首次提出了结构功能原理“使用情景”的形象认识.通过对教学实践和创新应用实践的分析,强调了训练和触发形象思维能力,可以有效地、显著地提高创新思维能力.%Uniqueness, foundation and fountainhead of thinking in images in innovation education are analyzed. Innovation ability is one type of social attributes and can be improved through practice and cultivation. Imaginal recognition of usage scenario to structural function principles is initially proposed. Practice and triggering of imaginal thinking ability, which can effectively and markedly enhance innovation thinking ability, are emphasized under the analysis of teaching practices and innovation applied practices.

  15. Statistical Analysis of the Cultivating Methods of Construction Workers' Safe Behavior Habits%建筑工人安全行为习惯培养方法的统计分析

    Institute of Scientific and Technical Information of China (English)

    杨高升; 居婕

    2013-01-01

    建筑工人的不安全行为直接导致了安全事故的发生,从行为心理学的角度分析了建筑工人不安全行为形成机理,并从中找出影响因素.细分安全行为习惯的培养方法:正强化、负强化、惩罚、教育培训,并通过对南京市两家建筑单位的管理人员进行问卷抽样调查,利用SPSS软件对安全行为习惯培养的四种方法进行统计分析,其中安全教育培训最为重要,结合国外安全培训模式经验,提出可行性的措施.%The construction workers unsafe behavior is the direct cause of security incidents. Analyzes the formation mechanism of construction workers' unsafe behavior from the perspective of behavior psychology, and finds out the factors. Subdivides the cultivating methods of safe behavior habits into four sectors, namely, positive reinforcement, negative reinforcement, punishment, and training. Through the questionnaire survey on the management of the two building units in Nanjing and using the SPSS software to conduct the statistical analysis of the four methods of fostering safe behavior habits, this paper finds that the safe education and training are the most important factors to foster safe behavior habits. At last, this paper gives the feasible measures combined with foreign experience in safety training mode.

  16. An improved bit shuffling pixels-based image scrambling method

    Institute of Scientific and Technical Information of China (English)

    ZHAO Hong; WANG Hong-xia; WANG Jin

    2011-01-01

    @@ Compared with the Arnold transform, the image scrambling method based on bit shuffling pixels is much more secure, and has higher efficiency and speed.However, the key space of this bit shuffling pixels based method is too small to resist exhaustive search attack.Therefore, an improved method based on chaos is proposed in this paper.The security of the improved scheme is enhanced by increasing the number of the keys.Theoretical analysis and experimental results show that the proposed method is effective and has higher security.

  17. Online Fault Diagnosis Method Based on Nonlinear Spectral Analysis

    Institute of Scientific and Technical Information of China (English)

    WEI Rui-xuan; WU Li-xun; WANG Yong-chang; HAN Chong-zhao

    2005-01-01

    The fault diagnosis based on nonlinear spectral analysis is a new technique for the nonlinear fault diagnosis, but its online application could be limited because of the enormous compution requirements for the estimation of general frequency response functions. Based on the fully decoupled Volterra identification algorithm, a new online fault diagnosis method based on nonlinear spectral analysis is presented, which can availably reduce the online compution requirements of general frequency response functions. The composition and working principle of the method are described, the test experiments have been done for damping spring of a vehicle suspension system by utilizing the new method, and the results indicate that the method is efficient.

  18. A New Robust Image Matching Method Based on Distance Reciprocal

    Institute of Scientific and Technical Information of China (English)

    赵春江; 施文康; 邓勇

    2004-01-01

    Object matching between two-dimensional images is an important problem in computer vision. The purpose of object matching is to decide the similarity between two objects. A new robust image matching method based on distance reciprocal was presented. The distance reciprocal is based on human visual perception. This method is simple and effective. Moreover, it is robust against noise. The experiments show that this method outperforms the Hausdorff distance, when the images with noise interfered need to be recognized.

  19. Method for detecting software anomalies based on recurrence plot analysis

    OpenAIRE

    Michał Mosdorf

    2012-01-01

    Presented paper evaluates method for detecting software anomalies based on recurrence plot analysis of trace log generated by software execution. Described method for detecting software anomalies is based on windowed recurrence quantification analysis for selected measures (e.g. Recurrence rate - RR or Determinism - DET). Initial results show that proposed method is useful in detecting silent software anomalies that do not result in typical crashes (e.g. exceptions).

  20. Method for detecting software anomalies based on recurrence plot analysis

    Directory of Open Access Journals (Sweden)

    Michał Mosdorf

    2012-03-01

    Full Text Available Presented paper evaluates method for detecting software anomalies based on recurrence plot analysis of trace log generated by software execution. Described method for detecting software anomalies is based on windowed recurrence quantification analysis for selected measures (e.g. Recurrence rate - RR or Determinism - DET. Initial results show that proposed method is useful in detecting silent software anomalies that do not result in typical crashes (e.g. exceptions.

  1. Power quality events recognition using a SVM-based method

    Energy Technology Data Exchange (ETDEWEB)

    Cerqueira, Augusto Santiago; Ferreira, Danton Diego; Ribeiro, Moises Vidal; Duque, Carlos Augusto [Department of Electrical Circuits, Federal University of Juiz de Fora, Campus Universitario, 36036 900, Juiz de Fora MG (Brazil)

    2008-09-15

    In this paper, a novel SVM-based method for power quality event classification is proposed. A simple approach for feature extraction is introduced, based on the subtraction of the fundamental component from the acquired voltage signal. The resulting signal is presented to a support vector machine for event classification. Results from simulation are presented and compared with two other methods, the OTFR and the LCEC. The proposed method shown an improved performance followed by a reasonable computational cost. (author)

  2. Wind Turbine Gearbox Fault Diagnosis Method Based on Riemannian Manifold

    OpenAIRE

    Shoubin Wang; Xiaogang Sun; Chengwei Li

    2014-01-01

    As multivariate time series problems widely exist in social production and life, fault diagnosis method has provided people with a lot of valuable information in the finance, hydrology, meteorology, earthquake, video surveillance, medical science, and other fields. In order to find faults in time sequence quickly and efficiently, this paper presents a multivariate time series processing method based on Riemannian manifold. This method is based on the sliding window and uses the covariance mat...

  3. Efficient Option Pricing Methods Based on Fourier Series Expansions

    Institute of Scientific and Technical Information of China (English)

    Deng DING; Sio Chong U

    2011-01-01

    A novel option pricing method based on Fourier-cosine series expansion was proposed by Fang and Oosterlee. Developing their idea, three new option pricing methods based on Fourier, Fourier-cosine and Fourier-sine series expansions are presented in this paper, which are more efficient when the option prices are calculated with many strike prices. A series of numerical experiments under different exp-Lévy models are also given to compare these new methods with the Fang and Oosterlee's method and other methods.

  4. Correlation theory-based signal processing method for CMF signals

    Science.gov (United States)

    Shen, Yan-lin; Tu, Ya-qing

    2016-06-01

    Signal processing precision of Coriolis mass flowmeter (CMF) signals affects measurement accuracy of Coriolis mass flowmeters directly. To improve the measurement accuracy of CMFs, a correlation theory-based signal processing method for CMF signals is proposed, which is comprised of the correlation theory-based frequency estimation method and phase difference estimation method. Theoretical analysis shows that the proposed method eliminates the effect of non-integral period sampling signals on frequency and phase difference estimation. The results of simulations and field experiments demonstrate that the proposed method improves the anti-interference performance of frequency and phase difference estimation and has better estimation performance than the adaptive notch filter, discrete Fourier transform and autocorrelation methods in terms of frequency estimation and the data extension-based correlation, Hilbert transform, quadrature delay estimator and discrete Fourier transform methods in terms of phase difference estimation, which contributes to improving the measurement accuracy of Coriolis mass flowmeters.

  5. 白假丝酵母菌简便培养方法的建立及临床应用研究%The constrution and clinical application of convenient Candida albicans cultivation method

    Institute of Scientific and Technical Information of China (English)

    王晓玲; 侯佳宜; 高颖; 杨少芳; 任建平

    2014-01-01

    目的:探讨真菌简便培养方法的临床应用价值。方法收集2006年1月-2013年12月我院门诊及住院患者疑似真菌感染标本,建立真菌简便培养基对收集标本进行培养,并将其与法国梅里埃培养基进行比较;对比新鲜自制简便培养基与2~8℃放置6个月后简便培养基的真菌检出率。结果在2011-2013年收集的307份标本中,简便培养基白假丝酵母菌的检出率为38.4%,而梅里埃培养基的检出率为26.1%,自制简便培养基对阴道分泌物标本、痰标本白假丝酵母菌检出率优于梅里埃培养基,且差异均有统计学意义(P均<0.05),而尿标本和咽拭子标本中两种方法白假丝酵母菌的检出率差异均无统计学意义(P均>0.05)。白假丝酵母菌在两种培养基上的菌落形态相似,但梅里埃培养基上白假丝酵母菌菌落显示蓝色,而自制简便培养基白假丝酵母菌菌落呈奶油色,并且均能抑制大部分杂菌的生长。痰标本、阴道分泌物标本及其他标本的白假丝酵母菌检出率在新鲜与2~8℃放置6个月后培养基中差异均无统计学意义(P均>0.05)。在2006-2010年共2840份阴道分泌物标本中,检出白假丝酵母菌以外的其他致病菌475份,阳性检出率为16.7%(475/2840),其中白假丝酵母菌与其他致病菌双重感染者有87例,阳性检出率为3.1%(87/2840)。结论真菌简便培养基制作简单、成本低廉,对白假丝酵母菌有良好选择性,可向农村基层医疗机构推广使用。%Objective To study the clinical application value of a convenient fungus cultivation method. Methods The suspected samples of fungus infection from the clinic service and hospitalization pa-tients in our hospital from January 2006 to December 2013 were collected. The fungus infection samples were detected by convenient fungus cultivation method. The cultivated results of homemade

  6. Cultivation of English Comprehensive Competences Based on Students' Majors%基于专业人才培养定位的大学英语综合应用能力培养

    Institute of Scientific and Technical Information of China (English)

    李俊波

    2012-01-01

    本文从社会需要出发分析了专业人才的英语素质和大学英语综合应用能力培养过程中的问题,提出了搞好大学英语自身特色建设的建议,倡导基于专业人才培养定位的大学英语综合应用能力培养。%This paper analyses professional talents' English skills required from the view of society and work situation as well as the problems in the process of college English education with regard to students' English comprehensive competences. It puts forward sev- eral suggestions to improve College English education, proposing cultivation of English comprehensive competences based on students' majors

  7. Study on Stratification Cultivation of Automobile after-sale Service Talents in Higher Vocational Colleges Based on AHP%基于AHP法的高职汽车售后服务人才分层培养研究

    Institute of Scientific and Technical Information of China (English)

    鲍婷婷

    2014-01-01

    汽车售后服务人才是影响汽车维修企业发展的核心要素。运用AHP法对汽车售后服务人才所需要的能力进行分析,从专业技能、社会技能和概念技能三个方面构建人才评价体系,并提出了相应的分层培养策略。%Automobile after-sale service talents are the key factor of the development of automobile repair enterprises. This paper an⁃alyzes the abilities required by automobile after-sale service talents based on AHP. The talents evaluating system is constructed from the three aspects of professional skills, social skills and conceptual skills, and corresponding tactics for stratification cultivation are put forward.

  8. Forest cover dynamics of shifting cultivation in the Democratic Republic of Congo: a remote sensing-based assessment for 2000-2010

    Science.gov (United States)

    Molinario, G.; Hansen, M. C.; Potapov, P. V.

    2015-09-01

    Shifting cultivation has traditionally been practiced in the Democratic Republic of Congo by carving agricultural fields out of primary and secondary forest, resulting in the rural complex: a characteristic land cover mosaic of roads, villages, active and fallow fields and secondary forest. Forest clearing has varying impacts depending on where it occurs relative to this area: whether inside it, along its primary forest interface, or in more isolated primary forest areas. The spatial contextualization of forest cover loss is therefore necessary to understand its impacts and plan its management. We characterized forest clearing using spatial models in a Geographical Information System, applying morphological image processing to the Forets d’Afrique Central Evaluee par Teledetection product. This process allowed us to create forest fragmentation maps for 2000, 2005 and 2010, classifying previously homogenous primary forest into separate patch, edge, perforated, fragmented and core forest subtypes. Subsequently we used spatial rules to map the established rural complex separately from isolated forest perforations, tracking the growth of these areas in time. Results confirm that the expansion of the rural complex and forest perforations has high variance throughout the country, with consequent differences in local impacts on forest ecology and habitat fragmentation. Between 2000 and 2010 the rural complex grew by 10.2% (46 182 ha), increasing from 11.9% to 13.1% of the total land area (1.2% change) while perforated forest grew by 74.4% (23 856 ha), from 0.8% to 1.5%. Core forest decreased by 3.8% (54 852 ha), from 38% to 36.6% of the 2010 land area. Of particular concern is the nearly doubling of perforated forest, a land dynamic that represents greater spatial intrusion of forest clearing within core forest areas and a move away from the established rural complex.

  9. Highly sensitive methods for electroanalytical chemistry based on nanotubule membranes.

    Science.gov (United States)

    Kobayashi, Y; Martin, C R

    1999-09-01

    Two new methods of electroanalysis are described. These methods are based on membranes containing monodisperse Au nanotubules with inside diameters approaching molecular dimensions. In one method, the analyte species is detected by measuring the change in trans-membrane current when the analyte is added to the nanotubule-based cell. The second method entails the use of a concentration cell based on the nanotubule membrane. In this case, the change in membrane potential is used to detect the analyte. Detection limits as low as 10(-11) M have been achieved. Hence, these methods compete with even the most sensitive of modern analytical methodologies. In addition, excellent molecular-sized-based selectivity is observed.

  10. Kernel based eigenvalue-decomposition methods for analysing ham

    DEFF Research Database (Denmark)

    Christiansen, Asger Nyman; Nielsen, Allan Aasbjerg; Møller, Flemming

    2010-01-01

    conditions and finding useful additives to hinder the color to change rapidly. To be able to prove which methods of storing and additives work, Danisco wants to monitor the development of the color of meat in a slice of ham as a function of time, environment and ingredients. We have chosen to use multi...... methods, such as PCA, MAF or MNF. We therefore investigated the applicability of kernel based versions of these transformation. This meant implementing the kernel based methods and developing new theory, since kernel based MAF and MNF is not described in the literature yet. The traditional methods only...... have two factors that are useful for segmentation and none of them can be used to segment the two types of meat. The kernel based methods have a lot of useful factors and they are able to capture the subtle differences in the images. This is illustrated in Figure 1. You can see a comparison of the most...

  11. Map-based control method for vehicle stability enhancement

    Institute of Scientific and Technical Information of China (English)

    Moon-Young Yoon; Seung-Hwan Baek; Kwang-Suk Boo; Heung-Seob Kim

    2015-01-01

    This work proposes a map-based control method to improve a vehicle’s lateral stability, and the performance of the proposed method is compared with that of the conventional model-referenced control method. Model-referenced control uses the sliding mode method to determine the compensated yaw moment;in contrast, the proposed map-based control uses the compensated yaw moment map acquired by vehicle stability analysis. The vehicle stability region is calculated by a topological method based on the trajectory reversal method. A 2-DOF vehicle model and Pacejka’s tire model are used to evaluate the proposed map-based control method. The properties of model-referenced control and map-based control are compared under various road conditions and driving inputs. Model-referenced control uses a control input to satisfy the linear reference model, and it generates unnecessary tire lateral forces that may lead to worse performance than an uncontrolled vehicle with step steering input on a road with a low friction coefficient. However, map-based control determines a compensated yaw moment to maintain the vehicle within the stability region, so the typical responses of vehicle enable to converge rapidly. The simulation results with sine and step steering show that map-based control provides better the tracking responsibility and control performance than model-referenced control.

  12. Dynamics of decadal changes in the distribution of double-cropping rice cultivation in China

    Institute of Scientific and Technical Information of China (English)

    DUAN JuQi; ZHOU GuangSheng

    2013-01-01

    Quantitative description of changes in the distribution of paddy rice cultivation in response to recent climate change provides a reference for rice cultivation patterns and formulation of countermeasures to cope with future climate change in China.This study analyzes the dynamics of decadal changes in distribution of double-cropping rice in China during 1961-2010 in relation to climate change based on the maximum entropy method.Decadal changes in the double-cropping rice cultivation area and climatic suitability in China were apparent.The total area of climatically suitable regions was highest in the 1960s,and subsequently showed an increasing trend at first and then a decreasing trend from the 1970s to 2000s.However,the low climatic suitability area decreased,which implied that the moderate and high climatic suitability areas increased.Among the latter,the high climatic suitability area showed the highest increase in extent to 4.4 times that of the 1990s and four times that of the 1960s.The areas of double-cropping rice cultivation most sensitive to climate change are mainly located in central Jiangsu,central Anhui,the eastern Sichuan Basin,southern Henan and central Guizhou.Transformation of areas between low and moderate climatic suitability was observed in northern Zhejiang,southern Anhui and Hubei,and northern Guangxi.Transformation of areas between moderate and high climatic suitability was observed in central Jiangxi and Leizhou Peninsula.The northern boundary of double-cropping rice cultivation in China shifted southwards and contracted eastwards in the 1970s,and extended northwards in the 1980s.However,the northern boundary did not shift northwards in response to climate warming in the 2000s.

  13. 不同耕作方式对土壤物理性状及玉米产量的影响%Effects of Different Cultivation Methods on the Soil Physical Properties and Yield of Maize

    Institute of Scientific and Technical Information of China (English)

    吕巨智; 程伟东; 钟昌松; 范继征; 石达金; 刘永红; 闫飞燕

    2014-01-01

    The purpose of this experiment was to identify the best cultivation method of maize and provide references for enhancing yield of maize cultivars. To study the effects of different tillage methods on soil physical properties and yield with different growing stages, a field experiment was conducted with randomized block design. The effects of different tillage methods on soil moisture content, bulk density, porosity in 0-45 cm soil depth varied with different growing stages. Among all the 6 tillage methods, the effect of 25-35 cm subsoiling depth on those physical properties mentioned above was the most obvious. In addition, after the subsoiling treatment, maize yield components, such as corn ear length, ear diameter, barren-tip length, number of rows per ear, kernels per row and 100-grain weight were improved at different degrees compared with CK. The maize yield with subsoiling treatment was 0.06%-3.13%higher than CK, but not significantly. Subsoiling could increase soil moisture, decrease soil bulk density, increase the porosity, function as water conservation and increase maize yield. Among them, the combination of 35 cm subsoiling depth and rotary tillage was the most optimal.%为寻求最佳耕作方式、提高玉米品种产量提供理论依据,采用随机区组设计,研究6种不同耕作方式对土壤物理性状和玉米产量的影响。不同耕作方式对不同生育时期0~45 cm土壤的含水量、容重、孔隙度影响各不同,在不同耕作方式中以深松25~35 cm效果最为明显;深松处理的玉米穗长、穗粗、秃尖长、穗行数、行粒数、百粒重等产量构成因素均较对照有不同程度的改善;深松处理比对照玉米增产0.06%~3.13%,但是增产幅度不是很明显。深松处理可以提高土壤含水量,降低容重,增加孔隙度,起到蓄水保墒作用。以深松25~35 cm为最优组合。

  14. Breeding management method of long-term artificial cultivation of tree shrew in beijing area%北京地区实验树鼩的饲养管理方法

    Institute of Scientific and Technical Information of China (English)

    赵琰; 吴婷婷; 李翼飞; 孙晔; 孙慧; 胡丽娜; 屈会化; 王庆国

    2013-01-01

    树鼩作为低等灵长类动物,在生物学研究中的价值日益引起研究者重视,将其作为实验动物的实验需求越来越多.作为南方亚热带动物,树鼩如何适应北方地区的生活是北方地区将其用作实验动物的前提.本实验室探索出适宜树鼩居住的饲养笼具,确定了饲养环境温度、湿度、光照时间、噪声及通风等条件,制定了规范化的卫生管理标准,建立了北京地区长期人工培育树鼩的饲养管理方法,为今后利用树鼩进行中医药病证结合动物模型的研究奠定了基础.%Tree shrew, a species of lower primates, its value in biomedical research has draw attention of researchers, and have been more and more demanded as a laboratory animal. However, tree shrew as a southern subtropical animal, how to adapt it to live in northern China becomes the premise that tree shrew can be used as a laboratory animal in northern China. Our laboratory has explored feeding cage, which is suitable for tree shrew living, determined feeding environmental conditions, such as temperature, humidity, illumination time, noise, ventilation, etc. , formulated the health management standard and established breeding management method of long-term artificial cultivation of tree shrew in Beijing area. Therefore, we have laid the foundation for the application of tree shrew in research on traditional Chinese medicine syndrome combined with establishment of disease animal models in the future.

  15. A Spatialization-based Method for Checking and Updating Metadata

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    In this paper the application of spatialization technology on metadata quality check and updating was discussed. A new method based on spatialization was proposed for checking and updating metadata to overcome the deficiency of text based methods with the powerful functions of spatial query and analysis provided by GIS software. This method employs the technology of spatialization to transform metadata into a coordinate space and the functions of spatial analysis in GIS to check and update spatial metadata in a visual environment. The basic principle and technical flow of this method were explained in detail, and an example of implementation using ArcMap of GIS software was illustrated with a metadata set of digital raster maps. The result shows the new method with the support of interaction of graph and text is much more intuitive and convenient than the ordinary text based method, and can fully utilize the functions of GIS spatial query and analysis with more accuracy and efficiency.

  16. Multi-pattern Matching Methods Based on Numerical Computation

    Directory of Open Access Journals (Sweden)

    Lu Jun

    2013-01-01

    Full Text Available Multi-pattern matching methods based on numerical computation are advanced in this paper. Firstly it advanced the multiple patterns matching algorithm based on added information. In the process of accumulating of information, the select method of byte-accumulate operation will affect the collision odds , which means that the methods or bytes involved in the different matching steps should have greater differences as much as possible. In addition, it can use balanced binary tree to manage index to reduce the average searching times, and use the characteristics of a given pattern set by setting the collision field to eliminate collision further. In order to reduce the collision odds in the initial step, the information splicing method is advanced, which has greater value space than added information method, thus greatly reducing the initial collision odds. Multiple patterns matching methods based on numerical computation fits for large multi-pattern matching.

  17. Conceptual bases of the brand valuation by cost method

    Directory of Open Access Journals (Sweden)

    G.Y. Studinska

    2015-03-01

    Full Text Available The necessity of valuing intangible assets in accordance with international trends is substantiated. The brand is seen as more important component of intangible assets, as an effective management tool company. The benefits and uses of brand evaluation results are investigated. System monocriterion cost brand evaluation methods is analyzed. In particular, methods that require evaluation by the time factor (current and forecast methods and methods for factor comparison base (relative and absolute. The cost method of brand valuation through market transactions in accordance J.Common’s classification is considered in detail. The explanation of the difference between method a summation of all costs and method of brand valuation through market transactions is provided. The advantages and disadvantages considered cost method of brand valuation are investigated. The cost method as the relative-predicted of the brand valuation, «The method of determining the proportion of the brand from the discounted total costs» is grounded

  18. Application of Remote Sensing Techniques for Mapping Shifting Cultivation

    Directory of Open Access Journals (Sweden)

    N.Vijaya Kumari

    2017-01-01

    Full Text Available Shifting cultivation is an ancient and primitive method of cultivation, also referred to as ‘slash and burn’ or ‘rotational bush fallow agricultural system’. The practice mainly involves removal of the forests by primitive slash and burn technique followed by mixed cropping for short period before abandoning the site. Tribals all over India are known to follow the practice of shifting cultivation with some regional variations. The present study has been undertaken to estimate the spatial distribution of podu cultivation in Srikakulam district of Andhra Pradesh by using remote sensing techniques. Results of the present study indicate that an estimated area of 10,491.857 acres of the forest area is under shifting cultivation in Srikakulam district. Shifting cultivation has caused a great deal of environmental degradation. Remote sensing techniques with repetitive coverage and synoptic view provide database for assessing environment degrading practices. Mapping of shifting areas is important not only from ecological point of view but also for management purposes.

  19. 医学院校学生的跨文化交际能力培养策略探析--基于主题式单元教学模式%A study on cultivating strategy of medical students’ intercultural communication competence at college level---Based on the theme-based unit teaching

    Institute of Scientific and Technical Information of China (English)

    褚宏蕊; 金文

    2013-01-01

    In the context of globalization, medical personnel with high intercultural communicative competence has become necessary of social development, this paper compares the differences between the traditional teaching mode and the theme of unit teaching mode, analyzes the inevitable demand of the cultivation of intercultural communicative competence, put forward methods of intercultural communicative competence of medical students training thematic unit based on the teaching mode in English teaching in university, to pay attention to groups of scholars concern and education and medical students of intercultural communicative competence.%在全球化的大背景下,具有较高跨文化交际能力的医学人才也成为社会发展的必需,本文通过对比传统教学模式与主题式单元教学模式的差异,分析培养跨文化交际能力的必然需求,提出在大学英语教学中基于主题式单元教学模式培养医学生的跨文化交际能力的方法,旨在引起学者对此问题的关注以及教育者及医学生群体对跨文化交际能力的重视。

  20. A New Method for Riccati Differential Equations Based on Reproducing Kernel and Quasilinearization Methods

    Directory of Open Access Journals (Sweden)

    F. Z. Geng

    2012-01-01

    Full Text Available We introduce a new method for solving Riccati differential equations, which is based on reproducing kernel method and quasilinearization technique. The quasilinearization technique is used to reduce the Riccati differential equation to a sequence of linear problems. The resulting sets of differential equations are treated by using reproducing kernel method. The solutions of Riccati differential equations obtained using many existing methods give good approximations only in the neighborhood of the initial position. However, the solutions obtained using the present method give good approximations in a larger interval, rather than a local vicinity of the initial position. Numerical results compared with other methods show that the method is simple and effective.

  1. An Exploration on the Studio-based Cultivation of Innovation Ability for Students Majoring in Software Technology in Higher Vocational colleges%基于工作室的高职软件技术专业学生创新能力培养探索

    Institute of Scientific and Technical Information of China (English)

    覃国蓉; 刘志军; 张世华; 周德伟; 何涛

    2015-01-01

    高职软件技术专业学生创新能力的培养非常重要,但是由于创新能力培养是一个系统工程,虽然现有研究和实践作了很多努力和尝试但是效果都不理想.包豪斯教学思想在建筑、艺术和设计专业领域的取得了极大的成功,鉴于软件开发与建筑和艺术设计有诸多相似之处,本文提出了一个基于工作室来培养高职软件技术专业学生的创新能力的方法,分析了工作室对于培养学生创新能力的作用,实际应用证明了该方法的可行性.%The cultivation of innovation ability of software technology students in higher vocational colleges is of great importance. A lot of efforts and attempts have been made in the existing research and practice, but the result was not satisfying. In view of the fact that Bauhaus teaching thought in the field of architecture and art design has achieved great success and there are many similarities between software development and architecture or art design, This paper presents a method of training the innovation ability of the students of higher vocational software technology based on studio, and analyzes the function of the studio to cultivate the students innovation ability. The practical application proves the feasibility of the method.

  2. Fuzzy Clustering Method for Web User Based on Pages Classification

    Institute of Scientific and Technical Information of China (English)

    ZHAN Li-qiang; LIU Da-xin

    2004-01-01

    A new method for Web users fuzzy clustering based on analysis of user interest characteristic is proposed in this article.The method first defines page fuzzy categories according to the links on the index page of the site, then computes fuzzy degree of cross page through aggregating on data of Web log.After that, by using fuzzy comprehensive evaluation method, the method constructs user interest vectors according to page viewing times and frequency of hits, and derives the fuzzy similarity matrix from the interest vectors for the Web users.Finally, it gets the clustering result through the fuzzy clustering method.The experimental results show the effectiveness of the method.

  3. Theory-Based Lexicographical Methods in a Functional Perspective

    DEFF Research Database (Denmark)

    Tarp, Sven

    2014-01-01

    This contribution provides an overview of some of the methods used in relation to the function theory. It starts with a definition of the concept of method and the relation existing between theory and method. It establishes an initial distinction between artisanal and theory-based methods...... of various methods used in the different sub-phases of the overall dictionary compilation process, from the making of the concept to the preparation for publication on the chosen media, with focus on the Internet. Finally, it briefly discusses some of the methods used to create and test the function theory...

  4. Study on Cultivated Land Resource Security and Strategy in Anhui Province%安徽省耕地资源安全保障与对策研究

    Institute of Scientific and Technical Information of China (English)

    何蓓蓓; 何兵

    2011-01-01

    本文在界定耕地资源安全概念的基础上,对安徽省耕地资源安全现状进行分析,并引入耕地质量折算方法,从耕地资源安全需求与供给两个方面,更为科学地预测2020年全省的耕地资源安全态势.研究结果表明,到2020年质量折算后的耕地供给量约为574.19万hm2,高于预测的耕地安全需求量(551.54万hm2)近26万hm2,理论上尚可保障耕地资源安全,但实际仍面临很大的压力.最后,针对目前存在的问题提出对策建议.%Based on the definition of the concept for cultivated land resources security, the paper research on status of cultivated land resource security in Anhui. From demand and supply, in the research, the paper gives a more scientific prediction for security of cultivate land resources in 2020 by introducing the quality of cultivated land conversion method. The results show that by 2020, the cultivated land security supply is 5741900 hm2, more than forecast demand for cultivated land security (5515400 hm2) nearly 260000hm2. In theory, cultivate land resource security can be protected,but in fact, cultivate land security still faces significant pressure. In the end, the measure is proposed for the present problems in this paper.

  5. A Fast Leak Locating Method Based on Wavelet Transform

    Institute of Scientific and Technical Information of China (English)

    GE Chuanhu; YANG Hongying; YE Hao; WANG Guizeng

    2009-01-01

    The problem of leak location is actually a time delay estimation (TDE) problem. Since most exist-ing TDE methods may encounter the problem of high computational complexity when used for online leak location. This paper presents a fast leak locating method based on wavelet transform (WT). The method first gets a rough estimate of the time delay from the WT coefficients of the pressure signals at the largest scale, then keeps refining the estimate using WT coefficients on smaller and smaller scales. Quantitative analyses and test results based on real data show that the method reduces the computational complexity while main-taining the time delay estimation accuracy.

  6. Data Mining and Knowledge Discovery via Logic-Based Methods

    CERN Document Server

    Triantaphyllou, Evangelos

    2010-01-01

    There are many approaches to data mining and knowledge discovery (DM&KD), including neural networks, closest neighbor methods, and various statistical methods. This monograph, however, focuses on the development and use of a novel approach, based on mathematical logic, that the author and his research associates have worked on over the last 20 years. The methods presented in the book deal with key DM&KD issues in an intuitive manner and in a natural sequence. Compared to other DM&KD methods, those based on mathematical logic offer a direct and often intuitive approach for extracting easily int

  7. Image mosaic method based on SIFT features of line segment.

    Science.gov (United States)

    Zhu, Jun; Ren, Mingwu

    2014-01-01

    This paper proposes a novel image mosaic method based on SIFT (Scale Invariant Feature Transform) feature of line segment, aiming to resolve incident scaling, rotation, changes in lighting condition, and so on between two images in the panoramic image mosaic process. This method firstly uses Harris corner detection operator to detect key points. Secondly, it constructs directed line segments, describes them with SIFT feature, and matches those directed segments to acquire rough point matching. Finally, Ransac method is used to eliminate wrong pairs in order to accomplish image mosaic. The results from experiment based on four pairs of images show that our method has strong robustness for resolution, lighting, rotation, and scaling.

  8. Network Traffic Anomalies Identification Based on Classification Methods

    Directory of Open Access Journals (Sweden)

    Donatas Račys

    2015-07-01

    Full Text Available A problem of network traffic anomalies detection in the computer networks is analyzed. Overview of anomalies detection methods is given then advantages and disadvantages of the different methods are analyzed. Model for the traffic anomalies detection was developed based on IBM SPSS Modeler and is used to analyze SNMP data of the router. Investigation of the traffic anomalies was done using three classification methods and different sets of the learning data. Based on the results of investigation it was determined that C5.1 decision tree method has the largest accuracy and performance and can be successfully used for identification of the network traffic anomalies.

  9. Empirical or rational truffle cultivation? It is time to choose.

    Directory of Open Access Journals (Sweden)

    Gerard Chevalier

    2014-08-01

    Full Text Available Aim of study: The aim of this study was to finalize a new method of truffle cultivation in order to obtain an earlier, more regular and  sizeable production of high quality fruiting bodies. Area of study: The experimentation was carried out in France (country of Dordogne, south-western  France  and Italy (country of  Marches,   eastern central Italy for more than one  decade.Material and methods: For the first time the method is based on scientific data on truffle biology particularly: the dynamic system of mycorrhization by the truffle and by other fungi;  the saprophytic capability of the truffle; the ability of its mycelium for decomposing certain minerals and organic materials in the “brûlés”.The basic principle concern the work of the soil and the upkeep of the root system: to work the soil  immediately after the plantation of mycorrhizal seedlings, deeply enough, with adapted tools,  in order to do not compact the soil,  aerate it,  favour the production of deep fruiting bodies not exposed to high temperatures, dryness, frost, parasites… ;  cut accurately  the roots in order to regenerate them and consequently to provide food for the truffle mycelium.Main results: The result has been a new cultivation method designated “differentiated” and called “MRT”, with adapted work of the soil on the lines of plantation and upkeep of the grass between them,  to maintain the cohabitation between areas where the mycelium is present, from those where it is absent.Research highlights: Research is going on to improve   the techniques and particularly finalize tools more precise and more adapted for working the soil and maintaining  the root system in a  best way.  Keywords: truffle; production; nutrition; soil, root system; cultivation tools. 

  10. A simple procedure for preparing substrate for Pleurotus ostreatus cultivation.

    Science.gov (United States)

    Hernández, Daniel; Sánchez, José E; Yamasaki, Keiko

    2003-11-01

    The use of wooden crates for composting a mixture of 70% grass, (Digitaria decumbens), and 30% coffee pulp, combined with 2% Ca(OH)(2), was studied as a method for preparing substrate for the cultivation of Pleurotus ostreatus. Crate composting considerably modified the temperature pattern of the substrate in process, as compared to pile composting, where lower temperatures and less homogeneous distributions were observed. Biological efficiencies varied between 59.79% and 93% in the two harvests. Based on statistical analysis significant differences were observed between the treatments, composting times and in the interactions between these two factors. We concluded that it is possible to produce P. ostreatus on a lignocellulosic, non-composted, non-pasteurized substrate with an initial pH of 8.7, and that composting for two to three days improves the biological efficiency.

  11. Pupils Humanistic Qualities Cultivating Based on Reading and Teaching of Children′s Literature%基于儿童文学阅读教学的小学生人文素养培养

    Institute of Scientific and Technical Information of China (English)

    程雯; 程瑞

    2015-01-01

    For the implementation of"rule system"in children′s literature humanistic qualities,it pointed out that must comply with the development regular of children′s cognitive psychology.The specific strategies were as follows,combining to interactive between home and school,creating reading environment;cultivating reading habits,feeling reading pleasure;strengthening the guideness of methods,building reading system;highlighting teaching characteristics,combining in class with out of class;subliming"facts judgment",understanding human feelings.The practical results of M and N school confirmed that reading and teaching of children′s literature is an effective way to cultivate pupil humanistic qualities.%针对儿童文学人文素养中“规则体系”的实施,指出其必须符合儿童认知心理发展规律。具体策略是:家校联合互动、营造阅读氛围;培养阅读习惯、感受阅读快乐;加强方法指导、构建阅读体系;凸显教学特色、课堂内外结合;升华“事实判断”、感悟人文情怀。 M、N校的实践结果印证了儿童文学阅读教学是培养小学生人文素养的有效途径。

  12. On the Cultivation of Chinese Animation Industry Based on Crowd-funding Business Model%基于众筹商业模式的中国动漫产业培育研究

    Institute of Scientific and Technical Information of China (English)

    李也; 贺正楚; 潘红玉

    2015-01-01

    众筹作为一种企业融资模式,在国外被应用于文化产品的项目开发。从众筹商业模式的分析框架出发,借鉴国外成功经验,揭示中国动漫产业发展过程中的困境,探讨和创新我国动漫产业的培育模式。基于众筹商业模式的动漫产业培育研究,为动漫企业开发产品的经营方式,以及政府出台扶持动漫产业的政策,提供了新的理论参考和政策依据。%As a corporation financing model, Crowd-funding has been applied to develop cultural products in the western countries.This essay analyses the framework of the Crowd-funding business model and learns from the successful experience of for-eign countries, revealing the difficulties at the development of China’ s animation industry, discussing and updating cultivation pat-tern of China’ s animation industry.Based on the Crowd-funding business model, the cultivation study of animation industry provides a new theoretical reference and policy basis for the operation of the animation business product development as well as the support of the government policy.

  13. Soil nutrient content analysis and assessment for cultivation base of gastrodia elata blume in Dejiang%德江天麻种植基地土壤养分含量分析及评价

    Institute of Scientific and Technical Information of China (English)

    吴迪; 邓琴; 贾亚琪; 程志飞; 刘品祯; 曾宪强; 王坤; 曹永直; 邵先强

    2016-01-01

    对德江天麻种植基地土壤pH、养分含量调查和分析,结果表明:土壤属弱酸性环境,较适宜天麻种植要求;土壤有机质、全氮、碱解氮含量较为丰富,属土壤肥力2级水平,为天麻高品质、高产量提供天然优势;缓效钾、速效钾含量稍缺乏,属土壤肥力4级水平,可能会影响到天麻的正常生长.建议合理施用天麻专用化肥,适量补充钾肥、合理施用微生物肥料、科学合理规划种植、注重栽培管理.%By investigating and analyzing the pH value,nutrient content in Dejiang cultivation base of Gastrodia elata Blume. The results showed that soil was in acidic environment and it met the require-ments of planting. The concentration was more abundant and soil fertility with the second level,such as soil organic matter,total nitrogen,and available nitrogen. It offered natural advantages for high-quality,high yield of Gastrodia elata Blume in Dejiang. It was lack of slowly available and fast-avail-able potassium. And the soil fertility was in the fourth level. Sometimes it maybe have an effect of nor-mal growth. It recommended many ways in planting,such as reasonable application-specific fertiliz-er,the amount of added fertilizer,rational application of microbial fertilizers,scientific and rational planning of planting,cultivation and management focus.

  14. Mapping cultivable land from satellite imagery with clustering algorithms

    Science.gov (United States)

    Arango, R. B.; Campos, A. M.; Combarro, E. F.; Canas, E. R.; Díaz, I.

    2016-07-01

    Open data satellite imagery provides valuable data for the planning and decision-making processes related with environmental domains. Specifically, agriculture uses remote sensing in a wide range of services, ranging from monitoring the health of the crops to forecasting the spread of crop diseases. In particular, this paper focuses on a methodology for the automatic delimitation of cultivable land by means of machine learning algorithms and satellite data. The method uses a partition clustering algorithm called Partitioning Around Medoids and considers the quality of the clusters obtained for each satellite band in order to evaluate which one better identifies cultivable land. The proposed method was tested with vineyards using as input the spectral and thermal bands of the Landsat 8 satellite. The experimental results show the great potential of this method for cultivable land monitoring from remote-sensed multispectral imagery.

  15. Ellipse-based shape description and retrieval method

    Institute of Scientific and Technical Information of China (English)

    李向阳; 潘云鹤

    2002-01-01

    Using a group of ellipses to approach the shape contour, a new shape retrieval method is presented in this paper. In order to keep shape-based retrieval invariant to its position, orientation and size, the shape normalization method is presented. From our research, any closed shape contour can be uniquely decomposed into a group of ellipses, and the original shape contour can be re-constructed using the decomposed ellipses. The ellipse-based shape description and similar retrieval method is introduced in this paper. Based on ellipse's contribution to shape contour, the decomposed ellipses are parted into low-order ellipses and high-order ellipses. The low-order ellipses measure the macroscopic feature of a shape contour, and the high-order ellipses measure the microscopic feature. The two-phase shape matching method is given. Through the experiment test, our method has better shape retrieval effect.

  16. Cultivation and uses of cucurbits

    Science.gov (United States)

    Cultivated cucurbits have spread through trade and exploration from their respective Old and New World centers of origin to the six arable continents and are important in local, regional and world trade. Cucumber (Cucumis sativus L.), melon (Cucumis melo L.), pumpkin, squash and gourd (Cucurbita spp...

  17. Cultivating archives: meanings and identities

    NARCIS (Netherlands)

    E. Ketelaar

    2012-01-01

    By cultivating archives through successive activations, people and communities define their identities. In these activations, the meanings of archives are constructed and reconstructed. Archives are not a static artifact imbued with the record creator’s voice alone, but a dynamic process involving a

  18. Convergence of a residual based artificial viscosity finite element method

    KAUST Repository

    Nazarov, Murtazo

    2013-02-01

    We present a residual based artificial viscosity finite element method to solve conservation laws. The Galerkin approximation is stabilized by only residual based artificial viscosity, without any least-squares, SUPG, or streamline diffusion terms. We prove convergence of the method, applied to a scalar conservation law in two space dimensions, toward an unique entropy solution for implicit time stepping schemes. © 2012 Elsevier B.V. All rights reserved.

  19. Memristor Crossbar-based Hardware Implementation of IDS Method

    OpenAIRE

    Merrikh-Bayat, Farnood; Bagheri-Shouraki, Saeed; Rohani, Ali

    2010-01-01

    Ink Drop Spread (IDS) is the engine of Active Learning Method (ALM), which is the methodology of soft computing. IDS, as a pattern-based processing unit, extracts useful information from a system subjected to modeling. In spite of its excellent potential in solving problems such as classification and modeling compared to other soft computing tools, finding its simple and fast hardware implementation is still a challenge. This paper describes a new hardware implementation of IDS method based o...

  20. Elargissement de la base génétique de la principale espèce de cotonnier cultivé Gossypium hirsutum L. par la création et l'exploitation de lignées monosomiques d'addition

    Directory of Open Access Journals (Sweden)

    Sarr D.

    2009-01-01

    Full Text Available Genetic broadening of the main cultivated cotton species Gossypium hirsutum L. by creation and exploitation of monosomic alien addition lines. The genus Gossypium is composed of about forty wild diploïd species that constitute an important reservoir of interesting genes for the genetic improvement of Gossypium hirsutum L., the main cultivated cotton species. Creation of monosomic alien addition lines (MAAL, made up of plants having in addition to the chromosome set of the cultivated species one wild species' supernumerary chromosome, is an interesting way to exploit this diversity. Numerous constraints limit the creation of MAAL, among them the most important is doubtless the production of first generation derivatives from pentaploids obtained by backcrossing G. hirsutum with bispecific hexaploid hybrids made of the cultivated species tetraploid genome and the genome of a donor diploid species. Raising this impediment by appropriate techniques allows to develop MAAL offering the possibility to introgress finely traits of interest from diploid species and to better understand genomic relationships between species in the genus Gossypium. Identification and exploitation of these MAAL have been for a long time based on not very reliable morphological characteristics and on the use of classical cytogenetic techniques, very heavy to implement. Nowadays, the exploitation of MAAL benefits from the great advances registered in molecular biology through the development of DNA markers and molecular cytogenetics. These progresses make of MAAL a promising way for the genetic improvement of the main cultivated cotton species.

  1. Current situation for cultivating reflective thinking in English reading instruction--The influence of teaching models

    Institute of Scientific and Technical Information of China (English)

    Meng Qinglan

    2013-01-01

    Reflective thinking plays an essential role in cultivating students’creative ability. It’s essential to cultivate this ability in the process of reading instruction,while the present traditional English teaching models hinder its development. This paper tries to analyze the current situation in cultivating students’reflective thinking in English reading instruction from the traditional teaching models,in the hope of refreshing the teachers’consciousness on their teaching methods.

  2. 建设病案管理实训基地与创新人才培养模式的探讨%Construction of Medical Record Information Management Training Base and Discussion of Innovative Talents Cultivation

    Institute of Scientific and Technical Information of China (English)

    王宁; 邢春国; 段沁江; 徐芬; 吴良明

    2016-01-01

    Medical record information management is a key link in modern hospital management, medical record information management level directly affects the process of health care reform, the lagging development of medical record management in our country, the shortage of medical record management personnel, to cultivate high quality medical record information management talent is imminent. In this paper, through literature search, questionnaire survey method,in view of the medical institutions for medical record information management personnel diathesis and vocational skills, medical record information management personnel in the core ability is put forward systematic medical record information practice base construction plan, including field condition, equipment requirements, the informationization level, management mechanism, teaching organization and operation management, etc. Through the establishment of cooperation professional training base construction, teaching reform of collaboration, cooperation, cooperation practice and employment mechanism of university-enterprise cooperation, to create "fusion, learn to do fusion, the fusion" personnel training mode, improve students' professional quality of medical record information management, to meet the requirements of health informatization level continuously improve and meet the requirements of medical and health care reform.%病案信息管理是现代医院管理中的重要环节,病案信息管理的水平直接影响着医疗卫生改革的进程,我国病案管理发展滞后,病案管理人才队伍紧缺,培养高素质的病案信息管理人才迫在眉睫.通过文献检索,问卷调查等方法,针对医疗机构对病案信息管理人才素质要求及职业技能目标,围绕病案信息管理人才核心能力提出系统化病案信息实训基地建设方案,包括场地条件、设备要求、信息化水平、管理机制、教学组织和运行管理等.通过建立合作专业建设、合作教

  3. A New Nonlinear Compound Forecasting Method Based on ANN

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    In this paper the compound-forecasting method is discussed. The compound-forecasting method is one of the hotspots in the current predication. Firstly, the compound-forecasting method is introduced and various existing compound-forecasting methods arediscussed. Secondly, the Artificial Neural Network (ANN) is brought in compound-prediction research and a nonlinear compound-prediction model based on ANN is presented. Finally, inorder to avoid irregular weight, a new method is presented which uses principal component analyses to increase the availability of compound-forecasting information. Higherforecasting precision is achieved in practice.

  4. An Adaptive Background Subtraction Method Based on Kernel Density Estimation

    Directory of Open Access Journals (Sweden)

    Mignon Park

    2012-09-01

    Full Text Available In this paper, a pixel-based background modeling method, which uses nonparametric kernel density estimation, is proposed. To reduce the burden of image storage, we modify the original KDE method by using the first frame to initialize it and update it subsequently at every frame by controlling the learning rate according to the situations. We apply an adaptive threshold method based on image changes to effectively subtract the dynamic backgrounds. The devised scheme allows the proposed method to automatically adapt to various environments and effectively extract the foreground. The method presented here exhibits good performance and is suitable for dynamic background environments. The algorithm is tested on various video sequences and compared with other state-of-the-art background subtraction methods so as to verify its performance.

  5. A Channelization-Based DOA Estimation Method for Wideband Signals

    Directory of Open Access Journals (Sweden)

    Rui Guo

    2016-07-01

    Full Text Available In this paper, we propose a novel direction of arrival (DOA estimation method for wideband signals with sensor arrays. The proposed method splits the wideband array output into multiple frequency sub-channels and estimates the signal parameters using a digital channelization receiver. Based on the output sub-channels, a channelization-based incoherent signal subspace method (Channelization-ISM and a channelization-based test of orthogonality of projected subspaces method (Channelization-TOPS are proposed. Channelization-ISM applies narrowband signal subspace methods on each sub-channel independently. Then the arithmetic mean or geometric mean of the estimated DOAs from each sub-channel gives the final result. Channelization-TOPS measures the orthogonality between the signal and the noise subspaces of the output sub-channels to estimate DOAs. The proposed channelization-based method isolates signals in different bandwidths reasonably and improves the output SNR. It outperforms the conventional ISM and TOPS methods on estimation accuracy and dynamic range, especially in real environments. Besides, the parallel processing architecture makes it easy to implement on hardware. A wideband digital array radar (DAR using direct wideband radio frequency (RF digitization is presented. Experiments carried out in a microwave anechoic chamber with the wideband DAR are presented to demonstrate the performance. The results verify the effectiveness of the proposed method.

  6. A Channelization-Based DOA Estimation Method for Wideband Signals.

    Science.gov (United States)

    Guo, Rui; Zhang, Yue; Lin, Qianqiang; Chen, Zengping

    2016-07-04

    In this paper, we propose a novel direction of arrival (DOA) estimation method for wideband signals with sensor arrays. The proposed method splits the wideband array output into multiple frequency sub-channels and estimates the signal parameters using a digital channelization receiver. Based on the output sub-channels, a channelization-based incoherent signal subspace method (Channelization-ISM) and a channelization-based test of orthogonality of projected subspaces method (Channelization-TOPS) are proposed. Channelization-ISM applies narrowband signal subspace methods on each sub-channel independently. Then the arithmetic mean or geometric mean of the estimated DOAs from each sub-channel gives the final result. Channelization-TOPS measures the orthogonality between the signal and the noise subspaces of the output sub-channels to estimate DOAs. The proposed channelization-based method isolates signals in different bandwidths reasonably and improves the output SNR. It outperforms the conventional ISM and TOPS methods on estimation accuracy and dynamic range, especially in real environments. Besides, the parallel processing architecture makes it easy to implement on hardware. A wideband digital array radar (DAR) using direct wideband radio frequency (RF) digitization is presented. Experiments carried out in a microwave anechoic chamber with the wideband DAR are presented to demonstrate the performance. The results verify the effectiveness of the proposed method.

  7. A CT Image Segmentation Algorithm Based on Level Set Method

    Institute of Scientific and Technical Information of China (English)

    QU Jing-yi; SHI Hao-shan

    2006-01-01

    Level Set methods are robust and efficient numerical tools for resolving curve evolution in image segmentation. This paper proposes a new image segmentation algorithm based on Mumford-Shah module. The method is used to CT images and the experiment results demonstrate its efficiency and veracity.

  8. Noise reduction method based on weighted manifold decomposition

    Institute of Scientific and Technical Information of China (English)

    Gan Jian-Chao; Xiao Xian-Ci

    2004-01-01

    A noise reduction method based on weighted manifold decomposition is proposed in this paper, which does not need knowledge of the chaotic dynamics and choosing number of eigenvalues. The simulation indicates that the performance of this method can increase the signal-to-noise ratio of noisy chaotic time series.

  9. Competition assignment problem algorithm based on Hungarian method

    Institute of Scientific and Technical Information of China (English)

    KONG Chao; REN Yongtai; GE Huiling; DENG Hualing

    2007-01-01

    Traditional Hungarian method can only solve standard assignment problems, while can not solve competition assignment problems. This article emphatically discussed the difference between standard assignment problems and competition assignment problems. The kinds of competition assignment problem algorithms based on Hungarian method and the solutions of them were studied.

  10. Network Surrounding and Cultivation of Citizenship Consciousness——Based on the Theories of Deliberative Democracy%网络围观与公民意识培育——基于“审议民主”的视角

    Institute of Scientific and Technical Information of China (English)

    李艳

    2012-01-01

    As an emerging political participation way in the information age, network surrounding plays a positive role in cultivation of citizenship consciousness. But the virtual characteristic and case features of network surrounding cause lacking in discipline constrain of interact users' statements and actions and losing persistent power of cultivation of citizenship conscious- ness. Based on theories of Deliberative Democracy, there are three ways to eliminate adverse impact of network surrounding on the cultivation of citizenship consciousness. Firstly, "deliberative" should be encouraged in "Weak Public" to construct an ideal public expression space; Secondly, a communication platform should be built between "Strong Publics" and "Weak Publics" as to. absorb public opinions; Thirdly, "Strong Publics" should emphasize legitimacy and clarifying.

  11. A Semantic Retrieval Method Based on the Fuzzy Reasoning

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    This paper gives a semantic fuzzy retrieval method of multimedia object,discusses the principle of fuzzy semantic retrieval technique,presents a fuzzy reasoning mechanism based on the knowledge base,and designs the relevant reasoning algorithms.Researchful results have innovative significance.

  12. Novel orchid varieties cultivated

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ A research team led by Prof.DUAN Jun from the CAS South China Botanical Garden has been successful in breeding new orchid hybrids. One of them,Haraenopsis Nanzhi Pink (coming from Phalaenopsis Wedding Promenade and Haraella retrocalla) has been accepted as a new hybrid genus by the London-based Royal Horticulture Society (RHS). It is the first new hybrid genus successfully registered at RHS by orchid breeders from Chinese mainland.

  13. Role-based Integration Method of Enterprise Information System

    Institute of Scientific and Technical Information of China (English)

    YU Ming-hui; FEI Qi; CHEN Xue-guang

    2002-01-01

    This paper analyzes the current situation of enterprise information system and methods of system integration at first. Then a role-based analyzing method is proposed. It can help confirm the keystone of the construction of information system and the direction of system integration. At last, a case study on theintegration of material dispatching information system in a large-scale project is presented briefly. It shows that this new method is more effective than the others are.

  14. A New Video Coding Method Based on Improving Detail Regions

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The Moving Pictures Expert Group (MPEG) and H.263 standard coding method is widely used in video compression. However, the visual quality of detail regions such as eyes and mouth is not content in people at the decoder, as far as the conference telephone or videophone is concerned. A new coding method based on improving detail regions is presented in this paper. Experimental results show that this method can improve the visual quality at the decoder.

  15. Method of designing developable surface based on engineering requirement

    Institute of Scientific and Technical Information of China (English)

    YANG Ji-xin; LIU Zhe; LIU Jian

    2006-01-01

    The paper deals with the principle of envelope of a one-parameter plane family to design developable surfaces. Three methods of designing developable surfaces are presented. They are designing a developable surface based on one curve on it and its normal line, designing a developable surface based on two curves on it and designing a developable surface based on one curve and one surface. They meet the requirements of engineering fields.

  16. A method for selecting training samples based on camera response

    Science.gov (United States)

    Zhang, Leihong; Li, Bei; Pan, Zilan; Liang, Dong; Kang, Yi; Zhang, Dawei; Ma, Xiuhua

    2016-09-01

    In the process of spectral reflectance reconstruction, sample selection plays an important role in the accuracy of the constructed model and in reconstruction effects. In this paper, a method for training sample selection based on camera response is proposed. It has been proved that the camera response value has a close correlation with the spectral reflectance. Consequently, in this paper we adopt the technique of drawing a sphere in camera response value space to select the training samples which have a higher correlation with the test samples. In addition, the Wiener estimation method is used to reconstruct the spectral reflectance. Finally, we find that the method of sample selection based on camera response value has the smallest color difference and root mean square error after reconstruction compared to the method using the full set of Munsell color charts, the Mohammadi training sample selection method, and the stratified sampling method. Moreover, the goodness of fit coefficient of this method is also the highest among the four sample selection methods. Taking all the factors mentioned above into consideration, the method of training sample selection based on camera response value enhances the reconstruction accuracy from both the colorimetric and spectral perspectives.

  17. An Efficient Method for Reliability-based Multidisciplinary Design Optimization

    Institute of Scientific and Technical Information of China (English)

    Fan Hui; Li Weiji

    2008-01-01

    Design for modem engineering system is becoming multidisciplinary and incorporates practical uncertainties; therefore, it is necessary to synthesize reliability analysis and the multidiscipLinary design optimization (MDO) techniques for the design of complex engineering system. An advanced first order second moment method-based concurrent subspace optimization approach is proposed based on the comparison and analysis of the existing multidisciplinary optimization techniques and the reliability analysis methods. It is seen through a canard configuration optimization for a three-surface transport that the proposed method is computationally efficient and practical with the least modification to the current deterministic optimization process.

  18. Genetic diversity and population structure in cultivated sunflower and a comparison to its wild progenitor, Helianthus annuus L.

    Science.gov (United States)

    Mandel, J R; Dechaine, J M; Marek, L F; Burke, J M

    2011-09-01

    Crop germplasm collections are valuable resources for ongoing plant breeding efforts. To fully utilize such collections, however, researchers need detailed information about the amount and distribution of genetic diversity present within collections. Here, we report the results of a population genetic analysis of the primary gene pool of sunflower (Helianthus annuus L.) based on a broad sampling of 433 cultivated accessions from North America and Europe, as well as a range-wide collection of 24 wild sunflower populations. Gene diversity across the cultivars was 0.47, as compared with 0.70 in the wilds, indicating that cultivated sunflower harbors roughly two-thirds of the total genetic diversity present in wild sunflower. Population structure analyses revealed that wild sunflower can be subdivided into four genetically distinct population clusters throughout its North American range, whereas the cultivated sunflower gene pool could be split into two main clusters separating restorer lines from the balance of the gene pool. Use of a maximum likelihood method to estimate the contribution of the wild gene pool to the cultivated sunflower germplasm revealed that the bulk of the cultivar diversity is derived from two wild sunflower population genetic clusters that are primarily composed of individuals from the east-central United States, the same general region in which sunflower domestication is believed to have occurred. We also identified a nested subset of accessions that capture as much of the allelic diversity present within the sampled cultivated sunflower germplasm collection as possible. At the high end, a core set of 288 captured nearly 90% of the alleles present in the full set of 433, whereas a core set of just 12 accessions was sufficient to capture nearly 50% of the total allelic diversity present within this sample of cultivated sunflower.

  19. A HMM-Based Method for Vocal Fold Pathology Diagnosis

    Directory of Open Access Journals (Sweden)

    Vahid Majidnezhad

    2012-11-01

    Full Text Available Acoustic analysis is a proper method in vocal fold pathology diagnosis so that it can complement and in some cases replace the other invasive, based on direct vocal fold observations methods. There are different approaches for vocal fold pathology diagnosis. This paper presents a method based on hidden markov model which classifies speeches into two classes: the normal and the pathological. Two hidden markov models are trained based on these two classes of speech and then the trained models are used to classify the dataset. The proposed method is able to classify the speeches with an accuracy of 93.75%. The results of this algorithm provide insights that can help biologists and computer scientists design high-performance system for detection of vocal fold pathology diagnosis.

  20. WLAN indoor location method based on artificial neural network

    Institute of Scientific and Technical Information of China (English)

    Zhou Mu; Sun Ying; Xu Yubin; Deng Zhian; Meng Weixiao

    2010-01-01

    WLAN indoor location method based on artificial neural network (ANN) is analyzed.A three layer feed-forward ANN model offers the benefits of reducing time cost of the layout of an indoor location system, saving storage cost of the radio map establishment and enhancing real-time capacity in the on-line phase.According to the analysis of SNR distributions of recorded beacon signal samples and discussion about the multi-mode phenomenon, the one map method is proposed for the purpose of simplifying ANN input values and increasing location performances.Based on the simulations and comparison analysis with other two typical indoor location methods, K-nearest neighbor (KNN) and probability, the feasibility and effectiveness of ANN-based indoor location method are verified with average location error of 2.37m and location accuracy of 78.6% in 3m.

  1. A new earthquake location method based on the waveform inversion

    CERN Document Server

    Wu, Hao; Huang, Xueyuan; Yang, Dinghui

    2016-01-01

    In this paper, a new earthquake location method based on the waveform inversion is proposed. As is known to all, the waveform misfit function is very sensitive to the phase shift between the synthetic waveform signal and the real waveform signal. Thus, the convergence domain of the conventional waveform based earthquake location methods is very small. In present study, by introducing and solving a simple sub-optimization problem, we greatly expand the convergence domain of the waveform based earthquake location method. According to a large number of numerical experiments, the new method expands the range of convergence by several tens of times. This allows us to locate the earthquake accurately even from some relatively bad initial values.

  2. Patch nearfield acoustic holography based on the equivalent source method

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    On the basis of nearfield acoustic holography (NAH) based on the equivalent source method (ESM), patch NAH based on the ESM is proposed. The method overcomes the shortcoming in the conventional NAH that the hologram surface should be larger than the source surface. It need not to discretize the whole source and its measurement need not to cover the whole source. The measurement may be performed over the region of interest, and the reconstruction can be done in the region directly. The method is flexible in applications, stable in computation, and very easy to implement. It has good potential applications in engineering. The nu- merical simulations show the invalidity of the conventional NAH based on the ESM and prove the validities of the proposed method for reconstructing a partial source and the regularization for reducing the error effect of the pressure measured on the hologram surface.

  3. Patch nearfield acoustic holography based on the equivalent source method

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    On the basis of nearfield acoustic holography (NAH) based on the equivalent source method (ESM), patch NAH based on the ESM is proposed. The method overcomes the shortcoming in the conventional NAH that the hologram surface should be larger than the source surface. It need not to discretize the whole source and its measurement need not to cover the whole source. The measurement may be performed over the region of interest, and the reconstruction can be done in the region directly. The method is flexible in applications, stable in computation, and very easy to implement. It has good potential applications in engineering. The numerical simulations show the invalidity of the conventional NAH based on the ESM and prove the validities of the proposed method for reconstructing a partial source and the regularization for reducing the error effect of the pressure measured on the hologram surface.

  4. Key Updating Methods for Combinatorial Design Based Key Management Schemes

    Directory of Open Access Journals (Sweden)

    Chonghuan Xu

    2014-01-01

    Full Text Available Wireless sensor network (WSN has become one of the most promising network technologies for many useful applications. However, for the lack of resources, it is different but important to ensure the security of the WSNs. Key management is a corner stone on which to build secure WSNs for it has a fundamental role in confidentiality, authentication, and so on. Combinatorial design theory has been used to generate good-designed key rings for each sensor node in WSNs. A large number of combinatorial design based key management schemes have been proposed but none of them have taken key updating into consideration. In this paper, we point out the essence of key updating for the unital design based key management scheme and propose two key updating methods; then, we conduct performance analysis on the two methods from three aspects; at last, we generalize the two methods to other combinatorial design based key management schemes and enhance the second method.

  5. Cultivators of the future

    Directory of Open Access Journals (Sweden)

    Aleksandr I. Neklessa

    2016-01-01

    Full Text Available Objective to analyze the dependence of worldbuilding oiconomics on the formation of the European worldview to comprehend the role of elites and social mentality in the worldbuilding process and in relation to the formation of the Russian reality and its current state. Methods an integrated approach to the analysis determined the range of specific research methods systemic and value analysis from the standpoint of geoeconomics geoculture and geoanthropology hermeneutic comparative analysis philosophical reasoning. Results the paper substantiates the critical role of elites and knowledge systems in the genesis of the complicated organization of the society the features of worldbuilding practices in Catholic Protestant Orthodox mentality are defined the role of geoeconomics as a relevant concept for the analysis of global processes and Russian reality is highlighted. Scientific novelty the article considers the specifics of Russia39s destiny as a continental geoeconomic space introduces into scientific circulation the concept of geoanthropology to determine the role of anthropological factor in the totality of processes and situations that arise in the allocation and reallocation of human resources on the planet with regard to their quality characteristics. Practical significance the main provisions and conclusions of the research are relevant to the scientific research and pedagogical work as well as for the development of the methodology of the Social Sciences and Humanities. It is of interest when considering the prospects of Russia39s development in the challenging circumstances of the 21 century. nbsp

  6. 《组织行为学》案例教学与学生创新能力的培养%Organizational Behavior Case-based Teaching and cultivation of students' innovative ability

    Institute of Scientific and Technical Information of China (English)

    钟曼丽

    2011-01-01

    Cultivation of innovative ability is one of the main tasks of case - based teaching.This paper combines oneself engaged in management course of the case - base teaching practice,this paper discusses the organizational behavior case - based teaching throug%创新能力的培养是案例教学的主要任务之一。本文结合自己从事管理类课程案例教学的实践,探讨《组织行为学》案例教学中通过案例教学课程设计创新意识培养、教学组织创建新环境、和教学方法培养创新思维的各种途径及方法,对改善《组织行为学》课程案例教学、提高学生的创新能力提出了新的思路。

  7. On the Cultivation of Intensive Reading Skills

    Institute of Scientific and Technical Information of China (English)

    杨雪琴

    2008-01-01

    This paper concerns the importance of the cultivation of reading skills,the characteristics of intensive reading and how to cultivate the intensive reading skills.Since the focus of this papre is how to cultivate the intensive reading skills,we make a systematic exposition from three points: word study,grammar patterns and text.

  8. Integrated navigation method based on inertial navigation system and Lidar

    Science.gov (United States)

    Zhang, Xiaoyue; Shi, Haitao; Pan, Jianye; Zhang, Chunxi

    2016-04-01

    An integrated navigation method based on the inertial navigational system (INS) and Lidar was proposed for land navigation. Compared with the traditional integrated navigational method and dead reckoning (DR) method, the influence of the inertial measurement unit (IMU) scale factor and misalignment was considered in the new method. First, the influence of the IMU scale factor and misalignment on navigation accuracy was analyzed. Based on the analysis, the integrated system error model of INS and Lidar was established, in which the IMU scale factor and misalignment error states were included. Then the observability of IMU error states was analyzed. According to the results of the observability analysis, the integrated system was optimized. Finally, numerical simulation and a vehicle test were carried out to validate the availability and utility of the proposed INS/Lidar integrated navigational method. Compared with the test result of a traditional integrated navigation method and DR method, the proposed integrated navigational method could result in a higher navigation precision. Consequently, the IMU scale factor and misalignment error were effectively compensated by the proposed method and the new integrated navigational method is valid.

  9. 鱼-鸭-菜生态种养模式的方法与效益%Methods and Benefit of Fish-duck-water spinach Eco-cultivating and Breeding Model

    Institute of Scientific and Technical Information of China (English)

    肖建中; 吕耀平

    2011-01-01

    The fish-duck-water spinach co-cultivation system is an ecological fishery mode with high benefit. In co-cultivation system, the fish pond provides a clean activity place for the ducks, meanwhile the duck supplies an adequate source of manure for the fish; And the water spinach not only make the water clean and increases the dissolved oxygen in pond, but also prettifies it. Two treatments, fish-duck-water spinach co-cultivation model (0.53 hm2) and only breeding fish (0.53 hm2), were set in a duck farm in Shuiguan village, Chengguang town, Liandu district, Lishui county, Zhejiang province in 2010 to analyze the benefit of co-cultivation model. The pure output value of the co-cultivation pond and the control pond was 61 480 and 11 130 RMB respectively. The ratio of invest to output of them was 1.00:1.46 and 1.00:1.26 respectively. The results showed that the economic effect of the fish-duck-water spinach co-cultivation system was remarkable.%鱼、鸭、菜混养技术是一种效益较高的生态渔业模式.于2010年在浙江省丽水市莲都区城关镇水东村养鸭场进行了鱼、鸭、菜共育试验.结果表明,鱼池为鸭提供了清洁的活动场所,而鸭子排泄的粪便为养鱼提供了充足的肥源,水面的空心菜使水体清洁,提高溶氧,也使池塘美观;试验面积1.06 hm2,试验塘(面积为0.53 hm2)和对照塘(面积为0.53 hm2)净产值分别为61 480元和11 130元,投入产出比分别为1.00∶1.46和1.00∶1.26,鱼、鸭、菜混养的平均纯收入是单纯养鱼的5.5倍,经济效益显著.

  10. COMPUTER-AIDED ENVIRONMENTAL RISK ASSESSMENT OF POTATO CULTIVATION TECHNOLOGY USING OPENLCA SOFTWARE

    Directory of Open Access Journals (Sweden)

    Jerzy Ryszard Grudziński

    2016-11-01

    Full Text Available The article discusses the environmental impact of the tillage processes of potato production. The analysis was performed using a simplified LCA ( Life Cycle Assessment software. Depending on the purpose and the level of detail of the process analysis, the LCA technique of environmental management may require computer assistance. Even then, carrying it out is very complex, long and costly. The aim of the study was to explore the possibility of the use of open source software for a simplified life-cycle analysis of potato with LCA method. There was selected OpenLCA – GreenDelta TC GmbH, v.1.3.1 software. The diagrams of unit process streams and energy inputs incurred on the individual processes of cultivation were designed based on literature. There were compared environmental hazards of the two systems of potato cultivation: with using the plough and the simplified one in terms of atmospheric pollution with chemicals emitted by the engines of agricultural tractors in the course of the subsequent agricultural procedures. The study results confirmed the suitability of the software for the initial evaluation (screening of environmental hazards using LCA method. The software is compatible with both the open source databases and commercial databases. Environmental impacts of cultivation technologies are presented in a form of a graphic illustration of pollution emissions. Also, the problems hindering the use of the software are indicated.

  11. NONLINEAR DATA RECONCILIATION METHOD BASED ON KERNEL PRINCIPAL COMPONENT ANALYSIS

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    In the industrial process situation, principal component analysis (PCA) is a general method in data reconciliation.However, PCA sometime is unfeasible to nonlinear feature analysis and limited in application to nonlinear industrial process.Kernel PCA (KPCA) is extension of PCA and can be used for nonlinear feature analysis.A nonlinear data reconciliation method based on KPCA is proposed.The basic idea of this method is that firstly original data are mapped to high dimensional feature space by nonlinear function, and PCA is implemented in the feature space.Then nonlinear feature analysis is implemented and data are reconstructed by using the kernel.The data reconciliation method based on KPCA is applied to ternary distillation column.Simulation results show that this method can filter the noise in measurements of nonlinear process and reconciliated data can represent the true information of nonlinear process.

  12. Wind Turbine Gearbox Fault Diagnosis Method Based on Riemannian Manifold

    Directory of Open Access Journals (Sweden)

    Shoubin Wang

    2014-01-01

    Full Text Available As multivariate time series problems widely exist in social production and life, fault diagnosis method has provided people with a lot of valuable information in the finance, hydrology, meteorology, earthquake, video surveillance, medical science, and other fields. In order to find faults in time sequence quickly and efficiently, this paper presents a multivariate time series processing method based on Riemannian manifold. This method is based on the sliding window and uses the covariance matrix as a descriptor of the time sequence. Riemannian distance is used as the similarity measure and the statistical process control diagram is applied to detect the abnormity of multivariate time series. And the visualization of the covariance matrix distribution is used to detect the abnormity of mechanical equipment, leading to realize the fault diagnosis. With wind turbine gearbox faults as the experiment object, the fault diagnosis method is verified and the results show that the method is reasonable and effective.

  13. NETWORK INTRUSION DETECTION METHOD BASED ON RS-MSVM

    Institute of Scientific and Technical Information of China (English)

    Xiao Yun; Han Chongzhao; Zheng Qinghua; Zhang Junjie

    2006-01-01

    A new method called RS-MSVM (Rough Set and Multi-class Support Vector Machine) is proposed for network intrusion detection. This method is based on rough set followed by MSVM for attribute reduction and classification respectively. The number of attributes of the network data used in this paper is reduced from 41 to 30 using rough set theory. The kernel function of HVDM-RBF (Heterogeneous Value Difference Metric Radial Basis Function), based on the heterogeneous value difference metric of heterogeneous datasets, is constructed for the heterogeneous network data. HVDM-RBF and one-against-one method are applied to build MSVM. DARPA (Defense Advanced Research Projects Agency) intrusion detection evaluating data were used in the experiment. The testing results show that our method outperforms other methods mentioned in this paper on six aspects: detection accuracy, number of support vectors, false positive rate, false negative rate, training time and testing time.

  14. Cultivated Characters of Nandina domestica%南天竹栽培性状研究

    Institute of Scientific and Technical Information of China (English)

    李湉

    2009-01-01

    [Objective] This study was to systematically investigate the cultivated characters of Nandina domestica and to lay a foundation for breeding, cultivating, as well as its application in landscape. [Method] Using N.domestica populations from 13 different areas of Hunan Province as tested samples, the cultivated characters of N.domestica in both the cultivated type and wild type were systematically studied through field experiment and comparative analysis. [Resul] The phenological features of N.domestica from different areas and germplasm resources were basically identical; the growth patterns of cultivated type and wild type were also basically identical, but the cultivated type grew faster than the wild type; both the two types grew quickly at young stage, and either the annual growth of new shoots or the growth pattern of leaves confirmed the fitting curve of the Logistic equation. [Conclusion] This research provided basis for the exploitation and utilization the germplasm resources of N.domestica.

  15. Deciphering Community Structure of Methanotrophs Dwelling in Rice Rhizospheres of an Indian Rice Field Using Cultivation and Cultivation-Independent Approaches.

    Science.gov (United States)

    Pandit, Pranitha S; Rahalkar, Monali C; Dhakephalkar, Prashant K; Ranade, Dilip R; Pore, Soham; Arora, Preeti; Kapse, Neelam

    2016-04-01

    Methanotrophs play a crucial role in filtering out methane from habitats, such as flooded rice fields. India has the largest area under rice cultivation in the world; however, to the best of our knowledge, methanotrophs have not been isolated and characterized from Indian rice fields. A cultivation strategy composing of a modified medium, longer incubation time, and serial dilutions in microtiter plates was used to cultivate methanotrophs from a rice rhizosphere sample from a flooded rice field in Western India. We compared the cultured members with the uncultured community as revealed by three culture-independent methods. A novel type Ia methanotroph (Sn10-6), at the rank of a genus, and a putative novel species of a type II methanotroph (Sn-Cys) were cultivated from the terminal positive dilution (10(-6)). From lower dilution (10(-4)), a strain of Methylomonas spp. was cultivated. All the three culture-independent analyses, i.e., pmoA clone library, terminal restriction fragment length polymorphism (T-RFLP), and metagenomics approach, revealed the dominance of type I methanotrophs. Only metagenomic analysis showed significant presence of type II methanotrophs, albeit in lower proportion (37 %). All the three isolates showed relevance to the methanotrophic community as depicted by uncultured methods; however, the cultivated members might not be the most dominant ones. In conclusion, a combined cultivation and cultivation-independent strategy yielded us a broader picture of the methanotrophic community from rice rhizospheres of a flooded rice field in India.

  16. Color Restoration Method Based on Spectral Information Using Normalized Cut

    Institute of Scientific and Technical Information of China (English)

    Tetsuro Morimoto; Tohru Mihashi; Katsushi Ikeuchi

    2008-01-01

    This paper proposes a novel method for color restoration that can effectively apply accurate color based on spectral information to a segmented image using the normalized cut technique. Using the proposed method, we can obtain a digital still camera image and spectral information in different environments. Also, it is not necessary to estimate reflectance spectra using a spectral database such as other methods. The synthesized images are accurate and high resolution. The proposed method effectively works in making digital archive contents. Some experimental results are demonstrated in this paper.

  17. A perceptual hashing method based on luminance features

    Science.gov (United States)

    Luo, Siqing

    2011-02-01

    With the rapid development of multimedia technology, content based searching and image authentication has become strong requirements. Image hashing technique has been proposed to meet them. In this paper, an RST (Rotation, Scaling, and Translation) resistant image hash algorithm is presented. In this method, the geometric distortions are extracted and adjusted by normalization. The features of the image are generated from the high-rank moments of luminance distribution. With the help of the efficient image representation capability of high-rank moments, the robustness and discrimination of proposed method are improved. The experimental results show that the proposed method is better than some existing methods in robustness under rotation attack.

  18. FUZZY IDENTIFICATION METHOD BASED ON A NEW OBJECTIVE FUNCTION

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    A method of fuzzy identification based on a new objective function is proposed. The method could deal with the issue that input variables of a system have an effect on the input space while output variables of the system do not exert an influence on the input space in the proposed objective functions of fuzzy clustering. The method could simultaneously solve the problems about structure identification and parameter estimation; thus it makes the fuzzy model become optimal. Simulation example demonstrates that the method could identify non-linear systems and obviously improve modeling accuracy.

  19. A Novel Method for Solving KdV Equation Based on Reproducing Kernel Hilbert Space Method

    Directory of Open Access Journals (Sweden)

    Mustafa Inc

    2013-01-01

    Full Text Available We propose a reproducing kernel method for solving the KdV equation with initial condition based on the reproducing kernel theory. The exact solution is represented in the form of series in the reproducing kernel Hilbert space. Some numerical examples have also been studied to demonstrate the accuracy of the present method. Results of numerical examples show that the presented method is effective.

  20. A reservoir skeleton-based multiple point geostatistics method

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Traditional stochastic reservoir modeling,including object-based and pixel-based methods,cannot solve the problem of reproducing continuous and curvilinear reservoir objects. The paper first dives into the various stochastic modeling methods and extracts their merits,then proposes the skeleton-based multiple point geostatistics(SMPS) for the fluvial reservoir. The core idea is using the skeletons of reservoir objects to restrict the selection of data patterns. The skeleton-based multiple point geostatistics consists of two steps. First,predicting the channel skeleton(namely,channel centerline) by using the method in object-based modeling. The paper proposes a new method of search window to predict the skeleton. Then forecasting the distributions of reservoir objects using multiple point geostatistics with the restriction of channel skeleton. By the restriction of channel centerline,the selection of data events will be more reasonable and the realization will be achieved more really. The checks by the conceptual model and the real reservoir show that SMPS is much better than Sisim(sequential indicator simulation) ,Snesim(Single Normal Equation Simulation) and Simpat(simulation with patterns) in building the fluvial reservoir model. This new method will contribute to both the theoretical research of stochastic modeling and the oilfield developments of constructing highly precise reservoir geological models.

  1. NOVEL RADAR SIGNAL SORTING METHOD BASED ON GEOMETRIC COVERING

    Institute of Scientific and Technical Information of China (English)

    万建; 国强; 宋文明

    2013-01-01

    With the increase of complexity of electromagnetic environment and continuous appearance of advanced system radars ,signals received by radar reconnaissance receivers become even more intensive and complex .There-fore ,traditional radar sorting methods based on neural network algorithms and support vector machine (SVM ) cannot process them effectively .Aiming at solving this problem ,a novel radar signal sorting method based on the cloud model theory and the geometric covering algorithm is proposed .By applying the geometric covering algo-rithm to divide input signals into different covering domains based on their distribution characteristics ,the method can overcome a typical problem that it is easy for traditional sorting algorithms to fall into the local extrema due to the use of complex nonlinear equation to describe input signals .The method uses the cloud model to describe the membership degree between signals to be sorted and their covering domains ,thus it avoids the disadvantage that traditional sorting methods based on hard clustering cannot deinterleave the signal samples with overlapped param-eters .Experimental results show that the presented method can effectively sort advanced system radar signals with overlapped parameters in complex electromagnetic environment .

  2. A flower image retrieval method based on ROI feature

    Institute of Scientific and Technical Information of China (English)

    洪安祥; 陈刚; 李均利; 池哲儒; 张亶

    2004-01-01

    Flower image retrieval is a very important step for computer-aided plant species recognition. In this paper, we propose an efficient segmentation method based on color clustering and domain knowledge to extract flower regions from flower images. For flower retrieval, we use the color histogram of a flower region to characterize the color features of flower and two shape-based features sets, Centroid-Contour Distance (CCD) and Angle Code Histogram (ACH), to characterize the shape features of a flower contour. Experimental results showed that our flower region extraction method based on color clustering and domain knowledge can produce accurate flower regions. Flower retrieval results on a database of 885 flower images collected from 14 plant species showed that our Region-of-Interest (ROI) based retrieval approach using both color and shape features can perform better than a method based on the global color histogram proposed by Swain and Ballard (1991) and a method based on domain knowledge-driven segmentation and color names proposed by Das et al.(1999).

  3. A flower image retrieval method based on ROI feature

    Institute of Scientific and Technical Information of China (English)

    洪安祥; 陈刚; 李均利; 池哲儒; 张亶

    2004-01-01

    Flower image retrieval is a very important step for computer-aided plant species recognition.In this paper,we propose an efficient segmentation method based on color clustering and domain knowledge to extract flower regions from flower images.For flower retrieval,we use the color histogram of a flower region to characterize the color features of flower and two shape-based features sets,Centroid-Contour Distance(CCD)and Angle Code Histogram(ACH),to characterize the shape features of a flower contour.Experimental results showed that our flower region extraction method based on color clustering and domain knowledge can produce accurate flower regions.Flower retrieval results on a database of 885 flower images collected from 14 plant species showed that our Region-of-Interest(ROD based retrieval approach using both color and shape features can perform better than a method based on the global color histogram proposed by Swain and Ballard(1991)and a method based on domain knowledge-driven segmentation and color names proposed by Das et al.(1999).

  4. 基于生态足迹的甘肃省耕地资源可持续利用与情景预测%Sustainable use and scenario prediction of cultivated land in Gansu Province based on ecological footprint theory

    Institute of Scientific and Technical Information of China (English)

    刘秀丽; 张勃; 昝国江; 何旭强; 张调风

    2013-01-01

    基于生态足迹理论,测算了1997-2009年甘肃省在保持社会经济可持续发展下的最佳耕地资源容量,运用ARIMA模型对甘肃省2010-2015年的生态足迹和生态承载力进行预测,按照《甘肃省国民经济和社会发展第十二个五年规划纲要》中的社会经济指标对耕地资源可持续发展容量进行情景预测.结果表明:1997-2009年,甘肃省耕地资源可持续发展容量总体上呈现上升趋势,其中1997-2002年耕地资源可持续发展容量基本低于实际耕地面积,2003-2009年,耕地资源可持续发展容量均高于实际耕地面积,出现耕地利用负荷现象;ARIMA模型预测结果显示,2010-2015年,甘肃省人均耕地生态足迹呈现上升趋势,年平均增长率为4.75%,人均耕地生态承载力呈现减少趋势,但总体上变化不大,人均耕地生态赤字从2011年成为负值,并且逐渐增加;情景预测结果显示2010-2015年,甘肃省耕地资源可持续发展容量与实际耕地面积差距愈来愈大,预计到2015年,为实际耕地面积的2.42倍,耕地利用负荷现象严重,人地关系紧张.%Cultivated land resources, as parts of important natural resources, are the bases of existence and development of human society, which are also factors to the sustainable development of society. With the fast economic development and increasing population in China, the relationship between cultivated land resources and sustainable development of society is becoming more intensive. At the background of maintaining sustainable socio-economic development, using Ecological Footprint theory, the paper calculated the best capacity of cultivated land resources of Gansu province in from 1997 to 2009. And using the ARIMA model,it predicted the ecological footprint and ecological capacity of Gansu Province from 2010 to 2015. According to the socio-economic indicators in the 12th Five-Year Plan of Gansu Province, it gave a scenarios prediction of the sustainable

  5. Facial Feature Extraction Method Based on Coefficients of Variances

    Institute of Scientific and Technical Information of China (English)

    Feng-Xi Song; David Zhang; Cai-Kou Chen; Jing-Yu Yang

    2007-01-01

    Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) are two popular feature ex- traction techniques in statistical pattern recognition field. Due to small sample size problem LDA cannot be directly applied to appearance-based face recognition tasks. As a consequence, a lot of LDA-based facial feature extraction techniques are proposed to deal with the problem one after the other. Nullspace Method is one of the most effective methods among them. The Nullspace Method tries to find a set of discriminant vectors which maximize the between-class scatter in the null space of the within-class scatter matrix. The calculation of its discriminant vectors will involve performing singular value decomposition on a high-dimensional matrix. It is generally memory- and time-consuming. Borrowing the key idea in Nullspace method and the concept of coefficient of variance in statistical analysis we present a novel facial feature extraction method, i.e., Discriminant based on Coefficient of Variance (DCV) in this paper. Experimental results performed on the FERET and AR face image databases demonstrate that DCV is a promising technique in comparison with Eigenfaces, Nullspace Method, and other state-of-the-art facial feature extraction methods.

  6. 新课程实施中学生创新能力的培养%The Cultivation of Students' Innovative Ability in the Implementation of New Curriculum

    Institute of Scientific and Technical Information of China (English)

    顾成刚

    2011-01-01

    本文立足于新课程标准,探讨了通过营造创新的教学氛围、构建创新的课堂教学环境、培养创新的教学意识和采用创新的课堂教学方法来培养学生的创新精神和创新能力。%Based on new curriculum standard, this paper discusses how to cultivate students' innovative spirit and innovative ability by creating innovative teaching atmosphere, constructing innovative classroom teaching environment, cultivating innovative teaching awareness and adopting innovative classroom teaching methods.

  7. A hybrid method for pancreas extraction from CT image based on level set methods.

    Science.gov (United States)

    Jiang, Huiyan; Tan, Hanqing; Fujita, Hiroshi

    2013-01-01

    This paper proposes a novel semiautomatic method to extract the pancreas from abdominal CT images. Traditional level set and region growing methods that request locating initial contour near the final boundary of object have problem of leakage to nearby tissues of pancreas region. The proposed method consists of a customized fast-marching level set method which generates an optimal initial pancreas region to solve the problem that the level set method is sensitive to the initial contour location and a modified distance regularized level set method which extracts accurate pancreas. The novelty in our method is the proper selection and combination of level set methods, furthermore an energy-decrement algorithm and an energy-tune algorithm are proposed to reduce the negative impact of bonding force caused by connected tissue whose intensity is similar with pancreas. As a result, our method overcomes the shortages of oversegmentation at weak boundary and can accurately extract pancreas from CT images. The proposed method is compared to other five state-of-the-art medical image segmentation methods based on a CT image dataset which contains abdominal images from 10 patients. The evaluated results demonstrate that our method outperforms other methods by achieving higher accuracy and making less false segmentation in pancreas extraction.

  8. Construction of the Cultivation Model of Foreign Language Capacities for Medical Undergraduates Based on CBI Teaching Theory%CBI理论下的医学本科生外语能力培养模式的建构

    Institute of Scientific and Technical Information of China (English)

    黄国君

    2015-01-01

    The rapid development of modern medicine makes medical undergraduates have both basic knowledge of English and stronger abilities in academic research, including information retrieval, writing academic papers in English, participating in international academic conferences, applying for international projects and students exchange in international universities. The new English requirements for medical undergraduates promote the reform of the cultivation model of English capacities for medical undergraduates. Model of foreign language capacities for medical undergraduates will be intensively studied based on the content-based instruction theory so as to construct a teaching model with the triad of general English, general education and academic English.%社会发展对医学生外语能力提出的新要求推动了医学院校本科生外语能力培养模式的改革,以CBI(以内容为依托)理论为基础,对医学本科生外语能力培养模式进行研究和探索,建构基础英语、通识英语及学术英语结合的三位一体教学模式.

  9. Effects of different cultivation methods on stem apex yield and quality of sweetpotato for vegetable use under urban roof condition%屋顶种植条件下不同栽培方式对菜用甘薯茎尖产量及品质的影响

    Institute of Scientific and Technical Information of China (English)

    李欢; 贝嘉伟; 潘超; 赵伟东; 陆国权

    2016-01-01

    Summary Sweetpotato(Ipomoeabatatas)is an important upland crop in China,which plays an important role in feed and industrial raw materials.The root tuber has rich nutrition,and the aerial part of stems and leaves also has extremely high nutritive value.Recently,research on sweetpotato for vegetable use increased gradually.With the rapid development of urbanization process,the cultivated area decreased gradually,along with the decline in quantity and quality of agricultural products.However,the increased space of balcony and roof accelerated the development of balcony agriculture and urban roof agriculture.Meanwhile,new devices for planting become popular in daily life in cities.Sweetpotatoes grow faster in summer,and can solve the problem of leaf vegetables in short supply.Moreover,people can plant organic sweetpotato for vegetable use at home in a convenient and safe way. In this paper,three cultivation methods (soil cultivation,substrate cultivation and pipeline nutrient solution culture)and three cultivars (Pushu 53,Guangcaishu 5 and Fushu 18) of sweetpotato for vegetable use were selected for urban roof agriculture,to compare the impact of different cultivation methods on yield and quality of sweetpotato.In July 2014,stem cuttings from each of the three cultivars were planted under three cultivation methods in roof of Jixian Building in Zhejiang A & F University.Experiments were conducted in a randomized complete block design with three replications.Seedlings were examined and replanted in time after cutting to ensure a full stand of seedlings.Furthermore,control and prevention for pests should be conducted during the growth period,and sweetpotato tips (1 5 cm) were harvested after growth.The yield,water content,pigment content,soluble sugar content,soluble protein content,vitamin C content and nitrate content were determined in the lab. The results showed that the pipeline nutrient solution culture had significant advantages compared with soil cultivation or

  10. Testability integrated evaluation method based on testability virtual test data

    Institute of Scientific and Technical Information of China (English)

    Liu Guanjun; Zhao Chenxu; Qiu Jing; Zhang Yong

    2014-01-01

    Testability virtual test is a new test method for testability verification, which has the advantages such as low cost, few restrictions and large sample of test data. It can be used to make up the deficiency of testability physical test. In order to take the advantage of testability virtual test data effectively and to improve the accuracy of testability evaluation, a testability integrated eval-uation method is proposed in this paper based on testability virtual test data. Considering the char-acteristic of testability virtual test data, the credibility analysis method for testability virtual test data is studied firstly. Then the integrated calculation method is proposed fusing the testability vir-tual and physical test data. Finally, certain helicopter heading and attitude system is presented to demonstrate the proposed method. The results show that the testability integrated evaluation method is feasible and effective.

  11. A Method of Image Symmetry Detection Based on Phase Information

    Institute of Scientific and Technical Information of China (English)

    WU Jun; YANG Zhaoxuan; FENG Dengchao

    2005-01-01

    Traditional methods for detecting symmetry in image suffer greatly from the contrast of image and noise, and they all require some preprocessing. This paper presents a new method of image symmetry detection. This method detects symmetry with phase information utilizing logGabor wavelets, because phase information is stable and significant, while symmetric points produce patterns easy to be recognised and confirmable in local phase. Phase method does not require any preprocessing, and its result is accurate or invariant to contrast, rotation and illumination conditions. This method can detect mirror symmetry, rotating symmetry and curve symmetry at one time. Results of experiment show that, compared with pivotal element algorithm based on intensity information, phase method is more accurate and robust.

  12. International Conference on Robust Rank-Based and Nonparametric Methods

    CERN Document Server

    McKean, Joseph

    2016-01-01

    The contributors to this volume include many of the distinguished researchers in this area. Many of these scholars have collaborated with Joseph McKean to develop underlying theory for these methods, obtain small sample corrections, and develop efficient algorithms for their computation. The papers cover the scope of the area, including robust nonparametric rank-based procedures through Bayesian and big data rank-based analyses. Areas of application include biostatistics and spatial areas. Over the last 30 years, robust rank-based and nonparametric methods have developed considerably. These procedures generalize traditional Wilcoxon-type methods for one- and two-sample location problems. Research into these procedures has culminated in complete analyses for many of the models used in practice including linear, generalized linear, mixed, and nonlinear models. Settings are both multivariate and univariate. With the development of R packages in these areas, computation of these procedures is easily shared with r...

  13. PPA BASED PREDICTION-CORRECTION METHODS FOR MONOTONE VARIATIONAL INEQUALITIES

    Institute of Scientific and Technical Information of China (English)

    He Bingsheng; Jiang Jianlin; Qian Maijian; Xu Ya

    2005-01-01

    In this paper we study the proximal point algorithm (PPA) based predictioncorrection (PC) methods for monotone variational inequalities. Each iteration of these methods consists of a prediction and a correction. The predictors are produced by inexact PPA steps. The new iterates are then updated by a correction using the PPA formula. We present two profit functions which serve two purposes: First we show that the profit functions are tight lower bounds of the improvements obtained in each iteration. Based on this conclusion we obtain the convergence inexactness restrictions for the prediction step. Second we show that the profit functions are quadratically dependent upon the step lengths, thus the optimal step lengths are obtained in the correction step. In the last part of the paper we compare the strengths of different methods based on their inexactness restrictions.

  14. Three Methods for Occupation Coding Based on Statistical Learning

    Directory of Open Access Journals (Sweden)

    Gweon Hyukjun

    2017-03-01

    Full Text Available Occupation coding, an important task in official statistics, refers to coding a respondent’s text answer into one of many hundreds of occupation codes. To date, occupation coding is still at least partially conducted manually, at great expense. We propose three methods for automatic coding: combining separate models for the detailed occupation codes and for aggregate occupation codes, a hybrid method that combines a duplicate-based approach with a statistical learning algorithm, and a modified nearest neighbor approach. Using data from the German General Social Survey (ALLBUS, we show that the proposed methods improve on both the coding accuracy of the underlying statistical learning algorithm and the coding accuracy of duplicates where duplicates exist. Further, we find defining duplicates based on ngram variables (a concept from text mining is preferable to one based on exact string matches.

  15. Genomic comparisons of Brucella spp. and closely related bacteria using base compositional and proteome based methods

    DEFF Research Database (Denmark)

    Bohlin, Jon; Snipen, Lars; Cloeckaert, Axel

    2010-01-01

    , genomic codon and amino acid frequencies based comparisons) and proteomes (all-against-all BLAST protein comparisons and pan-genomic analyses). RESULTS: We found that the oligonucleotide based methods gave different results compared to that of the proteome based methods. Differences were also found...... than proteome comparisons between species in genus Brucella and genus Ochrobactrum. Pan-genomic analyses indicated that uptake of DNA from outside genus Brucella appears to be limited. CONCLUSIONS: While both the proteome based methods and the Markov chain based genomic signatures were able to reflect...

  16. Exploration on Ways of Talents Cultivation Based on Innovative Education at Higher Vocational College%基于创新教育的高职人才培养思路的探索

    Institute of Scientific and Technical Information of China (English)

    杨肖宁

    2015-01-01

    The importance of innovative talents is increasingly prominent in National Development Strategy with the fierce competition of comprehensive national power when entering into the 21st century. As the important base of talents training, colleges and universities play an important role in the process of cultivating innovative talents and constructing an innovative country. But the university talent-cultivating model in our country especially in higher vocational colleges still has a lot of problems unsuitable to the development of social economy. Due to this improper model the talents with weak creative ability lacking thoughts and motivations of diligent in thinking, venture in doubting and bravery in creating. Based on the view of problem solving, the writer of this paper believes that it is necessary to reform the present model and the paper provides some ways of exploring innovative talents at higher vocational colleges.%进入21世纪以来,随着国家间综合国力竞争的日趋激烈,创新型人才在国家发展战略中的重要性日渐突出.高校作为人才培养的重要基地,在培养创新型人才、建设创新型国家的进程中担负着重要使命.但是我国高校尤其是高职院校还存在许多不适应社会经济发展的地方,由此造成培养出来的人才创新能力不强,缺乏勤于思考、敢于怀疑、勇于创新的思想和动力.要解决这些问题,必须进行人才培养模式的改革.文章基于此出发点,对高职院校创新型人才培养的困境、原因、基本思路和措施等方面进行了探讨,为如何培养创新型人才提供了思路探索.

  17. Robust Speech Recognition Method Based on Discriminative Environment Feature Extraction

    Institute of Scientific and Technical Information of China (English)

    HAN Jiqing; GAO Wen

    2001-01-01

    It is an effective approach to learn the influence of environmental parameters,such as additive noise and channel distortions, from training data for robust speech recognition.Most of the previous methods are based on maximum likelihood estimation criterion. However,these methods do not lead to a minimum error rate result. In this paper, a novel discrimina-tive learning method of environmental parameters, which is based on Minimum ClassificationError (MCE) criterion, is proposed. In the method, a simple classifier and the Generalized Probabilistic Descent (GPD) algorithm are adopted to iteratively learn the environmental parameters. Consequently, the clean speech features are estimated from the noisy speech features with the estimated environmental parameters, and then the estimations of clean speech features are utilized in the back-end HMM classifier. Experiments show that the best error rate reduction of 32.1% is obtained, tested on a task of 18 isolated confusion Korean words, relative to a conventional HMM system.

  18. Analysis of Dynamic Modeling Method Based on Boundary Element

    Directory of Open Access Journals (Sweden)

    Xu-Sheng Gan

    2013-07-01

    Full Text Available The aim of this study was to study an improved dynamic modeling method based on a Boundary Element Method (BEM. The dynamic model was composed of the elements such as the beam element, plate element, joint element, lumped mass and spring element by the BEM. An improved dynamic model of a machine structure was established based on plate-beam element system mainly. As a result, the dynamic characteristics of a machine structure were analyzed and the comparison of computational results and experimental’s showed the modeling method was effective. The analyses indicate that the introduced method inaugurates a good way for analyzing dynamic characteristics of a machine structure efficiently.

  19. Image Mosaic Method Based on SIFT Features of Line Segment

    Directory of Open Access Journals (Sweden)

    Jun Zhu

    2014-01-01

    Full Text Available This paper proposes a novel image mosaic method based on SIFT (Scale Invariant Feature Transform feature of line segment, aiming to resolve incident scaling, rotation, changes in lighting condition, and so on between two images in the panoramic image mosaic process. This method firstly uses Harris corner detection operator to detect key points. Secondly, it constructs directed line segments, describes them with SIFT feature, and matches those directed segments to acquire rough point matching. Finally, Ransac method is used to eliminate wrong pairs in order to accomplish image mosaic. The results from experiment based on four pairs of images show that our method has strong robustness for resolution, lighting, rotation, and scaling.

  20. Local coding based matching kernel method for image classification.

    Directory of Open Access Journals (Sweden)

    Yan Song

    Full Text Available This paper mainly focuses on how to effectively and efficiently measure visual similarity for local feature based representation. Among existing methods, metrics based on Bag of Visual Word (BoV techniques are efficient and conceptually simple, at the expense of effectiveness. By contrast, kernel based metrics are more effective, but at the cost of greater computational complexity and increased storage requirements. We show that a unified visual matching framework can be developed to encompass both BoV and kernel based metrics, in which local kernel plays an important role between feature pairs or between features and their reconstruction. Generally, local kernels are defined using Euclidean distance or its derivatives, based either explicitly or implicitly on an assumption of Gaussian noise. However, local features such as SIFT and HoG often follow a heavy-tailed distribution which tends to undermine the motivation behind Euclidean metrics. Motivated by recent advances in feature coding techniques, a novel efficient local coding based matching kernel (LCMK method is proposed. This exploits the manifold structures in Hilbert space derived from local kernels. The proposed method combines advantages of both BoV and kernel based metrics, and achieves a linear computational complexity. This enables efficient and scalable visual matching to be performed on large scale image sets. To evaluate the effectiveness of the proposed LCMK method, we conduct extensive experiments with widely used benchmark datasets, including 15-Scenes, Caltech101/256, PASCAL VOC 2007 and 2011 datasets. Experimental results confirm the effectiveness of the relatively efficient LCMK method.

  1. Simple noise-reduction method based on nonlinear forecasting

    Science.gov (United States)

    Tan, James P. L.

    2017-03-01

    Nonparametric detrending or noise reduction methods are often employed to separate trends from noisy time series when no satisfactory models exist to fit the data. However, conventional noise reduction methods depend on subjective choices of smoothing parameters. Here we present a simple multivariate noise reduction method based on available nonlinear forecasting techniques. These are in turn based on state-space reconstruction for which a strong theoretical justification exists for their use in nonparametric forecasting. The noise reduction method presented here is conceptually similar to Schreiber's noise reduction method using state-space reconstruction. However, we show that Schreiber's method has a minor flaw that can be overcome with forecasting. Furthermore, our method contains a simple but nontrivial extension to multivariate time series. We apply the method to multivariate time series generated from the Van der Pol oscillator, the Lorenz equations, the Hindmarsh-Rose model of neuronal spiking activity, and to two other univariate real-world data sets. It is demonstrated that noise reduction heuristics can be objectively optimized with in-sample forecasting errors that correlate well with actual noise reduction errors.

  2. XML-based product information processing method for product design

    Science.gov (United States)

    Zhang, Zhen Yu

    2012-01-01

    Design knowledge of modern mechatronics product is based on information processing as the center of the knowledge-intensive engineering, thus product design innovation is essentially the knowledge and information processing innovation. Analysis of the role of mechatronics product design knowledge and information management features, a unified model of XML-based product information processing method is proposed. Information processing model of product design includes functional knowledge, structural knowledge and their relationships. For the expression of product function element, product structure element, product mapping relationship between function and structure based on the XML model are proposed. The information processing of a parallel friction roller is given as an example, which demonstrates that this method is obviously helpful for knowledge-based design system and product innovation.

  3. 信息化社会体育教育专业学生信息素养的培养%Research on the Cultivation of Physical Education Students' Information Literacy in Information-based Society

    Institute of Scientific and Technical Information of China (English)

    党林秀; 蒋宁; 刘永

    2012-01-01

    , this paper first puts forward to add some relevant curriculums, adjust the teaching content and methods of original curriculums; then builds up a good environment to cultivate the information accomplishment; lastly, attach the importance of cultivating the information accomplishment in every curriculum and improve the students' abilities of self-control and independent study. This paper aims at offering useful theory basis and practice' guidance for the development of Physical Education major thus cultivating graduates who can adapt to the information age.

  4. Method of infrared image enhancement based on histogram

    Institute of Scientific and Technical Information of China (English)

    WANG Liang; YAN Jie

    2011-01-01

    Aiming at the problem in infrared image enhancement, a new method is given based on histogram. Using the gray character- istics of target, the upper-bouod threshold is selected adaptively and the histogram is processed by the threshold. After choosing the gray transform function based on the gray level distribution of image, the gray transformation is done during histogram equalization. Finally, the enhanced image is obtained. Compared with histogram equalization (HE), histogram double equalization (HDE) and plateau histogram equalization (PE), the simulation results demonstrate that the image enhancement effect of this method has obvious superiority. At the same time, its operation speed is fast and real-time ability is excellent.

  5. Designing fuzzy inference system based on improved gradient descent method

    Institute of Scientific and Technical Information of China (English)

    Zhang Liquan; Shao Cheng

    2006-01-01

    The distribution of sampling data influences completeness of rule base so that extrapolating missing rules is very difficult. Based on data mining, a self-learning method is developed for identifying fuzzy model and extrapolating missing rules, by means of confidence measure and the improved gradient descent method. The proposed approach can not only identify fuzzy model, update its parameters and determine optimal output fuzzy sets simultaneously, but also resolve the uncontrollable problem led by the regions that data do not cover. The simulation results show the effectiveness and accuracy of the proposed approach with the classical truck backer-upper control problem verifying.

  6. Research of Stamp Forming Simulation Based on Finite Element Method

    Institute of Scientific and Technical Information of China (English)

    SU Xaio-ping; XU Lian

    2008-01-01

    We point out that the finite element method offers a greta functional improvement for analyzing the stamp forming process of an automobile panel. Using the finite element theory and the simulation method of sheet stamping forming, the element model of sheet forming is built based on software HyperMesh,and the simulation of the product's sheet forming process is analyzed based on software Dynaform. A series of simulation results are obtained. It is clear that the simulation results from the theoretical basis for the product's die design and are useful for selecting process parameters.

  7. A Robust Digital Watermark Extracting Method Based on Neural Network

    Institute of Scientific and Technical Information of China (English)

    GUOLihua; YANGShutang; LIJianhua

    2003-01-01

    Since watermark removal software, such as StirMark, has succeeded in washing watermarks away for most of the known watermarking systems, it is necessary to improve the robustness of watermarking systems. A watermark extracting method based on the error Back propagation (BP) neural network is presented in this paper, which can efficiently improve the robustness of watermarking systems. Experiments show that even if the watermarking systems are attacked by the StirMark software, the extracting method based on neural network can still efficiently extract the whole watermark information.

  8. Potential Energy Surfaces Using Algebraic Methods Based on Unitary Groups

    Directory of Open Access Journals (Sweden)

    Renato Lemus

    2011-01-01

    Full Text Available This contribution reviews the recent advances to estimate the potential energy surfaces through algebraic methods based on the unitary groups used to describe the molecular vibrational degrees of freedom. The basic idea is to introduce the unitary group approach in the context of the traditional approach, where the Hamiltonian is expanded in terms of coordinates and momenta. In the presentation of this paper, several representative molecular systems that permit to illustrate both the different algebraic approaches as well as the usual problems encountered in the vibrational description in terms of internal coordinates are presented. Methods based on coherent states are also discussed.

  9. CONSTRUCTION METHOD OF KNOWLEDGE MAP BASED ON DESIGN PROCESS

    Institute of Scientific and Technical Information of China (English)

    SU Hai; JIANG Zuhua

    2007-01-01

    Due to the increasing amount and complexity of knowledge in product design, the knowledge map based on design process is presented as a tool to reuse product design process, promote the product design knowledge sharing. The relationship between design task flow and knowledge flow is discussed; A knowledge organizing method based on design task decomposition and a visualization method to support the knowledge retrieving and sharing in product design are proposed. And a knowledge map system to manage the knowledge in product design process is built with Visual C++ and SVG. Finally, a brief case study is provided to illustrate the construction and application of knowledge map in fuel pump design.

  10. IDEF method-based simulation model design and development framework

    Directory of Open Access Journals (Sweden)

    Ki-Young Jeong

    2009-09-01

    Full Text Available The purpose of this study is to provide an IDEF method-based integrated framework for a business process simulation model to reduce the model development time by increasing the communication and knowledge reusability during a simulation project. In this framework, simulation requirements are collected by a function modeling method (IDEF0 and a process modeling method (IDEF3. Based on these requirements, a common data model is constructed using the IDEF1X method. From this reusable data model, multiple simulation models are automatically generated using a database-driven simulation model development approach. The framework is claimed to help both requirement collection and experimentation phases during a simulation project by improving system knowledge, model reusability, and maintainability through the systematic use of three descriptive IDEF methods and the features of the relational database technologies. A complex semiconductor fabrication case study was used as a testbed to evaluate and illustrate the concepts and the framework. Two different simulation software products were used to develop and control the semiconductor model from the same knowledge base. The case study empirically showed that this framework could help improve the simulation project processes by using IDEF-based descriptive models and the relational database technology. Authors also concluded that this framework could be easily applied to other analytical model generation by separating the logic from the data.

  11. Hybrid Fundamental Solution Based Finite Element Method: Theory and Applications

    OpenAIRE

    Changyong Cao; Qing-Hua Qin

    2015-01-01

    An overview on the development of hybrid fundamental solution based finite element method (HFS-FEM) and its application in engineering problems is presented in this paper. The framework and formulations of HFS-FEM for potential problem, plane elasticity, three-dimensional elasticity, thermoelasticity, anisotropic elasticity, and plane piezoelectricity are presented. In this method, two independent assumed fields (intraelement filed and auxiliary frame field) are employed. The formulations for...

  12. A robust tolerance design method based on process capability

    Institute of Scientific and Technical Information of China (English)

    曹衍龙; 杨将新; 吴昭同; 吴立群

    2004-01-01

    This paper presents a method for robust tolerance design in terms of Process Capability Indices (PCI) . The component tolerance and the suitable manufacturing processes can be selected based on the real manufacturing context. The robustness of design feasibility under the effect of uncertainties is also discussed. A comparison between the results obtained by the proposed model and other methods indicates that robust and reliable tolerance can be obtained.

  13. A robust tolerance design method based on process capability

    Institute of Scientific and Technical Information of China (English)

    CAO Yan-long(曹衍龙); YANG Jiang-xin(杨将新); WU Zhao-tong(吴昭同); WU Li-qun(吴立群)

    2004-01-01

    This paper presents a method for robust tolerance design in terms of Process Capability Indices (PCI). The component tolerance and the suitable manufacturing processes can be selected based on the real manufacturing context. The robustness of design feasibility under the effect of uncertainties is also discussed. A comparison between the results obtained by the proposed model and other methods indicates that robust and reliable tolerance can be obtained.

  14. Propagator-based methods for recursive subspace model identification

    OpenAIRE

    Mercère, Guillaume; Bako, Laurent; Lecoeuche, Stéphane

    2008-01-01

    International audience; The problem of the online identification of multi-input multi-output (MIMO) state-space models in the framework of discrete-time subspace methods is considered in this paper. Several algorithms, based on a recursive formulation of the MIMO output error state-space (MOESP) identification class, are developed. The main goals of the proposed methods are to circumvent the huge complexity of eigenvalues or singular values decomposition techniques used by the offline algorit...

  15. New de-interlacing method based on adaptive weight

    Institute of Scientific and Technical Information of China (English)

    赵建伟; 古雪丰; 王朋; 刘重庆

    2004-01-01

    De-interlacing is very important when converting interlaced pictures to progressive pictures in format conversion.Multi-formats digital broadcast and progressive display requires the de-interlacing technique. An adaptive weight deinterlacing method is proposed. It combines motion compensation technique with directional-based spatio-temporal filter efficiently. Experiment results indicate that the method can keep edge continuity and sharpness effectively, reduce the artifacts in motion areas, and shows better visual performance when the estimated motion vectors are inaccurate.

  16. Progress of DNA-based Methods for Species Identification

    Institute of Scientific and Technical Information of China (English)

    HU Zhen; ZHANG Su-hua; WANG Zheng; BIAN Ying-nan; LI Cheng-tao

    2015-01-01

    Species identification of biological samples is widely used in such fields as forensic science and food industry. A variety of accurate and reliable methods have been developed in recent years. The cur-rent reviewshows common target genes and screening criteria suitable for species identification, and de-scribed various DNA-based molecular biology methods about species identification. Additionally, it dis-cusses the future development of species identification combined with real-time PCR and sequencing technologies.

  17. How to Reach Evidence-Based Usability Evaluation Methods.

    Science.gov (United States)

    Marcilly, Romaric; Peute, Linda

    2017-01-01

    This paper discusses how and why to build evidence-based knowledge on usability evaluation methods. At each step of building evidence, requisites and difficulties to achieve it are highlighted. Specifically, the paper presents how usability evaluation studies should be designed to allow capitalizing evidence. Reciprocally, it presents how evidence-based usability knowledge will help improve usability practice. Finally, it underlines that evaluation and evidence participate in a virtuous circle that will help improve scientific knowledge and evaluation practice.

  18. The Efficient Method for Simultaneous Monitoring of the Culturable as Well as Nonculturable Airborne Microorganisms

    OpenAIRE

    2013-01-01

    Cultivation-based microbiological methods are a gold standard for monitoring of airborne micro-organisms to determine the occupational exposure levels or transmission paths of a particular infectious agent. Some highly contagious microorganisms are not easily culturable but it is becoming evident that cultivation and molecular methods are complementary and in these cases highly relevant. We report a simple and efficient method for sampling and analyzing airborne bacteria with an impactor-type...

  19. Restrictive Factors of Vocational Education Development in Cultivation of Rural Practical Skilled Personnel and Countermeasures

    OpenAIRE

    2014-01-01

    Kunming City is accelerating the process of agricultural modernization, industrialization, informationization and ecological development. In this process, it needs speeding up building new high level and advanced rural practical skilled personnel team suitable for development demand. By empirical analysis method, this paper discussed the factors restricting vocational education in cultivation of rural practical skilled personnel. Then, it came up with countermeasures in cultivation objective ...

  20. Model-based analysis of skill oriented labour management in a multi-operations and multiworker static cut rose cultivation system

    NARCIS (Netherlands)

    Ooster, van 't A.; Bontsema, J.; Henten, van E.J.; Hemming, S.

    2015-01-01

    Worldwide competitive challenges urge growers to further improve operational performance. In this paper, the objective ‘model-based analysis and improvement of the operation of horticultural production systems’ was narrowed to ranking simulated labour management scenarios in a multi-operations and m