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Sample records for based cultivation method

  1. Assessment of Cultivation Method for Energy Beet Based on LCA Method

    Institute of Scientific and Technical Information of China (English)

    Chunfeng; ZHANG; Feng; LIU; Yuangang; ZU; Qingying; MENG; Baoguo; ZHU; Nannan; WANG

    2014-01-01

    In order to establish a supply system for energy resource coupled with the environment,the production technology of sugar beets was explored as a biological energy source. The low-humic andosol as the experimental soil,the panting method was direct planting,and cultivation technique was minimum tillage direct planting method. The control was conventional tillage transplant and no tillage direct planting. The results demonstrated that data revealed that the energy cost of no tillage and a direct planting method was 105 GJ/hm2on average for two years,while that of the conventional tillage method was 112 GJ/hm2per year. The ratio of output to input showed that the direct planting with no tillage was more efficient( 3. 61) than the conventional tillage( 3. 01). Moreover,the emission of CO2into the atmosphere with no tillage and the direct planting was 71% of the conventional tillage planting technique. Therefore,direct planting without tillage reduces the impact on the environment.

  2. DNA-based stable isotope probing coupled with cultivation methods implicates Methylophaga in hydrocarbon degradation.

    Directory of Open Access Journals (Sweden)

    TonyGutierrez

    2014-02-01

    Full Text Available Marine hydrocarbon-degrading bacteria perform a fundamental role in the oxidation and ultimate removal of crude oil and its petrochemical derivatives in coastal and open ocean environments. Those with an almost exclusive ability to utilize hydrocarbons as a sole carbon and energy source have been found confined to just a few genera. Here we used stable isotope probing (SIP, a valuable tool to link the phylogeny and function of targeted microbial groups, to investigate hydrocarbon-degrading bacteria in coastal North Carolina sea water (Beaufort Inlet, USA with uniformly labeled [13C]n-hexadecane. The dominant sequences in clone libraries constructed from 13C-enriched bacterial DNA (from n-hexadecane enrichments were identified to belong to the genus Alcanivorax, with ≤98% sequence identity to the closest type strain – thus representing a putative novel phylogenetic taxon within this genus. Unexpectedly, we also identified 13C-enriched sequences in heavy DNA fractions that were affiliated to the genus Methylophaga. This is a contentious group since, though some of its members have been proposed to degrade hydrocarbons, substantive evidence has not previously confirmed this. We used quantitative PCR primers targeting the 16S rRNA gene of the SIP-identified Alcanivorax and Methylophaga to determine their abundance in incubations amended with unlabeled n-hexadecane. Both showed substantial increases in gene copy number during the experiments. Subsequently, we isolated a strain representing the SIP-identified Methylophaga sequences (99.9% 16S rRNA gene sequence identity and used it to show, for the first time, direct evidence of hydrocarbon degradation by a cultured Methylophaga sp. This study demonstrates the value of coupling SIP with cultivation methods to identify and expand on the known diversity of hydrocarbon-degrading bacteria in the marine environment.

  3. Advanced continuous cultivation methods for systems microbiology.

    Science.gov (United States)

    Adamberg, Kaarel; Valgepea, Kaspar; Vilu, Raivo

    2015-09-01

    Increasing the throughput of systems biology-based experimental characterization of in silico-designed strains has great potential for accelerating the development of cell factories. For this, analysis of metabolism in the steady state is essential as only this enables the unequivocal definition of the physiological state of cells, which is needed for the complete description and in silico reconstruction of their phenotypes. In this review, we show that for a systems microbiology approach, high-resolution characterization of metabolism in the steady state--growth space analysis (GSA)--can be achieved by using advanced continuous cultivation methods termed changestats. In changestats, an environmental parameter is continuously changed at a constant rate within one experiment whilst maintaining cells in the physiological steady state similar to chemostats. This increases the resolution and throughput of GSA compared with chemostats, and, moreover, enables following of the dynamics of metabolism and detection of metabolic switch-points and optimal growth conditions. We also describe the concept, challenge and necessary criteria of the systematic analysis of steady-state metabolism. Finally, we propose that such systematic characterization of the steady-state growth space of cells using changestats has value not only for fundamental studies of metabolism, but also for systems biology-based metabolic engineering of cell factories. PMID:26220303

  4. A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures

    Directory of Open Access Journals (Sweden)

    Glumoff Tuomo

    2010-02-01

    Full Text Available Abstract Background Cultivations for recombinant protein production in shake flasks should provide high cell densities, high protein productivity per cell and good protein quality. The methods described in laboratory handbooks often fail to reach these goals due to oxygen depletion, lack of pH control and the necessity to use low induction cell densities. In this article we describe the impact of a novel enzymatically controlled fed-batch cultivation technology on recombinant protein production in Escherichia coli in simple shaken cultures. Results The enzymatic glucose release system together with a well-balanced combination of mineral salts and complex medium additives provided high cell densities, high protein yields and a considerably improved proportion of soluble proteins in harvested cells. The cultivation method consists of three steps: 1 controlled growth by glucose-limited fed-batch to OD600 ~10, 2 addition of growth boosters together with an inducer providing efficient protein synthesis within a 3 to 6 hours period, and 3 a slow growth period (16 to 21 hours during which the recombinant protein is slowly synthesized and folded. Cell densities corresponding to 10 to 15 g l-1 cell dry weight could be achieved with the developed technique. In comparison to standard cultures in LB, Terrific Broth and mineral salt medium, we typically achieved over 10-fold higher volumetric yields of soluble recombinant proteins. Conclusions We have demonstrated that by applying the novel EnBase® Flo cultivation system in shaken cultures high cell densities can be obtained without impairing the productivity per cell. Especially the yield of soluble (correctly folded proteins was significantly improved in comparison to commonly used LB, Terrific Broth or mineral salt media. This improvement is thought to result from a well controlled physiological state during the whole process. The higher volumetric yields enable the use of lower culture volumes and can

  5. Identification of growth phases and influencing factors in cultivations with AGE1.HN cells using set-based methods.

    Directory of Open Access Journals (Sweden)

    Steffen Borchers

    Full Text Available Production of bio-pharmaceuticals in cell culture, such as mammalian cells, is challenging. Mathematical models can provide support to the analysis, optimization, and the operation of production processes. In particular, unstructured models are suited for these purposes, since they can be tailored to particular process conditions. To this end, growth phases and the most relevant factors influencing cell growth and product formation have to be identified. Due to noisy and erroneous experimental data, unknown kinetic parameters, and the large number of combinations of influencing factors, currently there are only limited structured approaches to tackle these issues. We outline a structured set-based approach to identify different growth phases and the factors influencing cell growth and metabolism. To this end, measurement uncertainties are taken explicitly into account to bound the time-dependent specific growth rate based on the observed increase of the cell concentration. Based on the bounds on the specific growth rate, we can identify qualitatively different growth phases and (in-validate hypotheses on the factors influencing cell growth and metabolism. We apply the approach to a mammalian suspension cell line (AGE1.HN. We show that growth in batch culture can be divided into two main growth phases. The initial phase is characterized by exponential growth dynamics, which can be described consistently by a relatively simple unstructured and segregated model. The subsequent phase is characterized by a decrease in the specific growth rate, which, as shown, results from substrate limitation and the pH of the medium. An extended model is provided which describes the observed dynamics of cell growth and main metabolites, and the corresponding kinetic parameters as well as their confidence intervals are estimated. The study is complemented by an uncertainty and outlier analysis. Overall, we demonstrate utility of set-based methods for analyzing cell

  6. Identification of growth phases and influencing factors in cultivations with AGE1.HN cells using set-based methods.

    Science.gov (United States)

    Borchers, Steffen; Freund, Susann; Rath, Alexander; Streif, Stefan; Reichl, Udo; Findeisen, Rolf

    2013-01-01

    Production of bio-pharmaceuticals in cell culture, such as mammalian cells, is challenging. Mathematical models can provide support to the analysis, optimization, and the operation of production processes. In particular, unstructured models are suited for these purposes, since they can be tailored to particular process conditions. To this end, growth phases and the most relevant factors influencing cell growth and product formation have to be identified. Due to noisy and erroneous experimental data, unknown kinetic parameters, and the large number of combinations of influencing factors, currently there are only limited structured approaches to tackle these issues. We outline a structured set-based approach to identify different growth phases and the factors influencing cell growth and metabolism. To this end, measurement uncertainties are taken explicitly into account to bound the time-dependent specific growth rate based on the observed increase of the cell concentration. Based on the bounds on the specific growth rate, we can identify qualitatively different growth phases and (in-)validate hypotheses on the factors influencing cell growth and metabolism. We apply the approach to a mammalian suspension cell line (AGE1.HN). We show that growth in batch culture can be divided into two main growth phases. The initial phase is characterized by exponential growth dynamics, which can be described consistently by a relatively simple unstructured and segregated model. The subsequent phase is characterized by a decrease in the specific growth rate, which, as shown, results from substrate limitation and the pH of the medium. An extended model is provided which describes the observed dynamics of cell growth and main metabolites, and the corresponding kinetic parameters as well as their confidence intervals are estimated. The study is complemented by an uncertainty and outlier analysis. Overall, we demonstrate utility of set-based methods for analyzing cell growth and

  7. Cultivation methods appliance in the micromycetes monitoring in water environment

    International Nuclear Information System (INIS)

    Micromycetes presence in the drinking water source can cause not only technological problems with drinking water treatment, but also can change its sensoric characteristic (taste, odour) and can have impact to the human health. Micromycetes monitoring in water environment demand cultivation. Cultivation specificities are needed to respect in all processes of water sample manipulation from sampling to the sanitary of the samples. Micromycetes are able to relatively well cultivated under laboratory condition. There are a lot of well known and certified formulates to micromycetes cultivation from native complexly media to synthetic media only. Different concerns in world produce cultivation media in large for using media according to the standard methods. Concerns as Difco, Oxoid, Himedia, Sanofi, Serva, Bio Merieux, Sartorius and in Slovakia Imuna befit within well-known industrial producers of cultivation media. Some of these media belongs to so called universal screening media another media are appropriate for taxonomic determination etc. These commercially produced cultivation media are applicable also to the fungi monitoring in the water environment. We are tested and compared different sampling and handling methods, different commercial produced cultivation media, and also different cultivation methods and results evaluation as the first step for hazard identification in risk assessment in water environment. (authors)

  8. An isotope approach based on C-13 pulse-chase labelling vs. the root trenching method to separate heterotrophic and autotrophic respiration in cultivated peatlands

    Energy Technology Data Exchange (ETDEWEB)

    Biasi, C.; Pitkamaki, A. S.; Tavi, N. M.; Koponen, H. T.; Martikainen, P. J. [Univ.of Eastern Finland, Kuopio (Finland). Dept. of Environmental Science], e-mail: christina.biasi@uef.fi

    2012-11-01

    We tested an isotope method based on C-13 pulse-chase labelling for determining the fractional contribution of soil microbial respiration to overall soil respiration in an organic soil (cutaway peatland, eastern Finland), cultivated with the bioenergy crop, reed canary grass. The plants were exposed to CO{sub 2}-13 for five hours and the label was thereafter determined in CO{sub 2} derived from the soil-root system. A two-pool isotope mixing model was used to separate sources of respiration. The isotopic approach showed that a minimum of 50% of the total CO{sub 2} originated from soil-microbial respiration. Even though the method uses undisturbed soil-plant systems, it has limitations concerning the experimental determination of the true isotopic signal of all components contributing to autotrophic respiration. A trenching experiment which was comparatively conducted resulted in a 71% fractional contribution of soil-microbial respiration. This value was likely overestimated. Further studies are needed to evaluate critically the output from these two partitioning approaches. (orig.)

  9. A Study on the Cultivation of College Students' Information Literacy Based on Task-driving Method%基于任务驱动法培养大学生信息素养的研究

    Institute of Scientific and Technical Information of China (English)

    丛荣华

    2011-01-01

    Through analyzing the significance of cultivating college students' information literacy and explaining the relation between information literacy and innovation ability training,this paper puts forward some strategies of cultivating college students' information literacy based on task-driving method.%本文通过对大学生信息素养培养意义的分析以及对信息素养与创新能力培养的关系的说明,详细论述了采用任务驱动法培养大学生信息素养的策略。

  10. Sampling method for monitoring classification of cultivated land in county area based on Kriging estimation error%基于Kriging估计误差的县域耕地等级监测布样方法

    Institute of Scientific and Technical Information of China (English)

    杨建宇; 汤赛; 郧文聚; 张超; 朱德海; 陈彦清

    2013-01-01

    China, an agricultural country, has a large population but not enough cultivated land. Until 2011, the cultivated land per capita was 1.38 mu (0.09 ha), only 40% of the world average, and it is getting worse with industrialization and urbanization. The next task for the Ministry of Land and Resources:Dynamic monitoring of cultivated land classification in which a number of counties will be sampled; in each county, a sample-based monitoring network would be established that reflects the distribution and its tendency of cultivated land classification in county area and estimates of non-sampled locations. Due to the correlation among samples, traditional methods such as simple random sampling, stratified sampling, and systematic sampling are insufficient to achieve the goal. Therefore, in this paper we introduced a spatial sampling method based on the Kriging estimation error. For our case, natural classifications of cultivated land identified from the last Land Resource Survey and Cultivated Land Evaluation are regarded as the true value and classifications of non-sampled cultivated lands would be predicted by interpolating the sample data. Finally, RMSE (root-mean-square error) of Kriging interpolation is redefined to measure the performance of the network. To be specific, five steps are needed for the monitoring network. First, the optimal sample size is determined by analyzing the variation trend between the number and the accuracy of samples. Then, set up the basic monitoring network using square grids. The suitable grid size can be chosen by comparing the grid sizes and the corresponding RMSEs from the Kriging interpolation of the samples data. Because some centers of grids do not overlap the area of cultivated land, the third step is to add some points near the centers of grids to create the global monitoring network. These points are selected from centroids of cultivated land spots which are closest to the centers and inside the searching circles around the

  11. Development and optimization of biofilm based algal cultivation

    Science.gov (United States)

    Gross, Martin Anthony

    This dissertation describes research done on biofilm based algal cultivation systems. The system that was developed in this work is the revolving algal biofilm cultivation system (RAB). A raceway-retrofit, and a trough-based pilot-scale RAB system were developed and investigated. Each of the systems significantly outperformed a control raceway pond in side-by-side tests. Furthermore the RAB system was found to require significantly less water than the raceway pond based cultivation system. Lastly a TEA/LCA analysis was conducted to evaluate the economic and life cycle of the RAB cultivation system in comparison to raceway pond. It was found that the RAB system was able to grow algae at a lower cost and was shown to be profitable at a smaller scale than the raceway pond style of algal cultivation. Additionally the RAB system was projected to have lower GHG emissions, and better energy and water use efficiencies in comparison to a raceway pond system. Furthermore, fundamental research was conducted to identify the optimal material for algae to attach on. A total of 28 materials with a smooth surface were tested for initial cell colonization and it was found that the tetradecane contact angle of the materials had a good correlation with cell attachment. The effects of surface texture were evaluated using mesh materials (nylon, polypropylene, high density polyethylene, polyester, aluminum, and stainless steel) with openings ranging from 0.05--6.40 mm. It was found that both surface texture and material composition influence algal attachment.

  12. Component analysis of cultivated ginseng, cultivated wild ginseng, and natural wild ginseng by structural parts using HPLC method

    OpenAIRE

    Young-Ju, Han; Ki-Rok, Kwon; Bae-Chun,Cha; Oh-Man,Kwon

    2007-01-01

    Objectives : The aim of this experiments is to provide an objective differentiation of ginseng, Korean and Chinese cultivated wild ginseng, and natural wild ginseng through components analysis of different parts of ginseng. Methods : Comparative analyses of ginsenoside-, ginsenoside-, and ginsenosides and from the root, stem, and leaves of ginseng, Korean and Chinese cultivated wild ginseng, and natural wild ginseng were conducted using HPLC. Results : 1. For content comparison of lea...

  13. Component analysis of cultivated ginseng, cultivated wild ginseng, and natural wild ginseng by structural parts using HPLC method

    Directory of Open Access Journals (Sweden)

    Young-Ju,Han

    2007-02-01

    Full Text Available Objectives : The aim of this experiments is to provide an objective differentiation of ginseng, Korean and Chinese cultivated wild ginseng, and natural wild ginseng through components analysis of different parts of ginseng. Methods : Comparative analyses of ginsenoside-, ginsenoside-, and ginsenosides and from the root, stem, and leaves of ginseng, Korean and Chinese cultivated wild ginseng, and natural wild ginseng were conducted using HPLC. Results : 1. For content comparison of leaves, ginseng showed highest content of ginsenoside than other samples. Natural wild ginseng showed relatively high content of ginsenosides and than other samples. 2. For content comparison of the stem, ginseng and 10 years old Chinese cultivated wild ginseng didn't contain ginsenoside . Natural wild ginseng showed higher content of ginsenosides and than other samples. 3. For content comparison of the root, ginsenoside was found only in 5 and 10 years old Korean cultivated wild ginseng. 4. Distribution of contents by the parts of ginseng was similar in ginseng and Chinese cultivated wild ginseng. Conclusions : Above experiment data can be an important indicator for the identification of ginseng, Korean and Chinese cultivated wild ginseng, and natural wild ginseng.

  14. Dynamics of flowering of artichoke globe (Cynara scolymus L.) plants in depending on cultivation method

    OpenAIRE

    Andrzej Sałata

    2012-01-01

    The study was carried out in the years 2001-2002 in the Felin Research Center in Lublin. The differences in growth dynamics and morphology of flowering shoots were investigated with regards to a method of cultivation of artichoke. In the year 2001 flowering shoots and flowers occurred the earliest in the year 2001 on artichoke plants cultivated from crowns. In the year 2002 plants cultivated from crowns and transplants produced flowering shoots in the same time. Artichoke plants cultivated fr...

  15. LTCC based bioreactors for cell cultivation

    Science.gov (United States)

    Bartsch, H.; Welker, T.; Welker, K.; Witte, H.; Müller, J.

    2016-01-01

    LTCC multilayers offer a wide range of structural options and flexibility of connections not available in standard thin film technology. Therefore they are considered as material base for cell culture reactors. The integration of microfluidic handling systems and features for optical and electrical capturing of indicators for cell culture growth offers the platform for an open system concept. The present paper assesses different approaches for the creation of microfluidic channels in LTCC multilayers. Basic functions required for the fluid management in bioreactors include temperature and flow control. Both features can be realized with integrated heaters and temperature sensors in LTCC multilayers. Technological conditions for the integration of such elements into bioreactors are analysed. The temperature regulation for the system makes use of NTC thermistor sensors which serve as real value input for the control of the heater. It allows the adjustment of the fluid temperature with an accuracy of 0.2 K. The tempered fluid flows through the cell culture chamber. Inside of this chamber a thick film electrode array monitors the impedance as an indicator for the growth process of 3-dimensional cell cultures. At the system output a flow sensor is arranged to monitor the continual flow. For this purpose a calorimetric sensor is implemented, and its crucial design parameters are discussed. Thus, the work presented gives an overview on the current status of LTCC based fluid management for cell culture reactors, which provides a promising base for the automation of cell culture processes.

  16. Integrated Indicators-based Gradation of Cultivated Land Quality in Mountainous Region of Southwestern China

    Institute of Scientific and Technical Information of China (English)

    SHAO Jing'an; WEI Chaofu; XIE Deti; ZHOU Yan

    2006-01-01

    The gradation of cultivated land is to assess the suitability of cultivated land for agricultural production in terms of natural and economic properties of land. It can be used to evaluate sustainability of land use and soil management practices. Formal and informal surveys, Delphi and comprehensive index method are adopted to identify appropriate integrated indicators, and measure the gradation of cultivated land quality. The determination of integrated indicators presents three basic features of cultivated land quality: stable plant productivity, social acceptability and maintenance of environmental quality.Pronounced concentration-dissipation law of total function score value occurs in paddy field units, with three ranges, I.e., 79-68.5, 68.5~59 and 59-51. Total function score value in dryland units mainly ranges from 40 to 70, but slight concentration-dissipation law of each unit is still observed, with four ranges, I.e., 79~68, 68~51, 51~37 and 37~15. Paddy field quality is divided into three gradations, and the scales are 18 220.9 ha, 5410.6 ha and 2890.9 ha. Dryland quality is classified into four gradations, and the scales are 1548.6 ha, 8153.8 ha, 3316.3 ha and 685.2 ha. The total function score value of every gradation unit is conformed to the results of farmers' assessment. Research results meet with the needs of accurate degree of the gradation of cultivated land quality. Integrated indicators-based gradation of cultivated land assessment accords with the characteristics of land resources and human preference in the mountains of southwestern China. This study will be useful to promote the monitoring of cultivated land quality, and to supply fine ground and knowledge for establishing appropriate cultivated land management practices in Southwestern China.

  17. Agent-based modelling of shifting cultivation field patterns, Vietnam

    DEFF Research Database (Denmark)

    Jepsen, Martin Rudbeck; Leisz, S.; Rasmussen, K.;

    2006-01-01

    modelling, and relying on empirical data from fieldwork and observations for parameterization of variables, the level of clustering in agricultural fields observed around a study village is reproduced. Agents in the model act to maximize labour productivity, which is based on potential yield and labour......Shifting cultivation in the Nghe An Province of Vietnam's Northern Mountain Region produces a characteristic land-cover pattern of small and larger fields. The pattern is the result of farmers cultivating either individually or in spatially clustered groups. Using spatially explicit agent-based...... costs associated with fencing of fields, and are faced with physical constraints. The simulation results are compared with land-cover data obtained from remote sensing. Comparisons are made on patterns as detected visually and using the mean nearest-neighbour ratio. Baseline simulation outputs show high...

  18. Component Analysis of Cultivated Ginseng, Red Ginseng, Cultivated Wild Ginseng, and Red Wild Ginseng Using HPLC Method

    Directory of Open Access Journals (Sweden)

    Jang Ho, Lee

    2008-06-01

    Full Text Available Objectives : The aim of this experiment is to provide an differentiation of ginseng, red ginseng, cultivated wild ginseng(CWG, and red wild ginseng(RWG through component analysis using HPLC(High Performance Liquid Chromatography, hereafter HPLC. Methods : Comparative analyses of ginsenoside Rg3, ginsenoside Rh2, and ginsenosides Rb1 and Rg1 of various ginsengs were conducted using HPLC. Results : 1. CWG was relatively heat-resistant and showed slow change in color during the process of steaming and drying, compared to cultivated ginseng. 2. Ginsenoside Rg3 was not detected in cultivated ginseng and CWG, whereas it was high in red ginseng and RWG. Ginsenoside Rg3 was more generated in red ginseng than in RWG. 3. Ginsenoside Rh2 appreared during steaming and drying of cultivated ginseng, whereas it was more increased during steaming and drying of CWG. 4. Ginsenoside Rg1 content was more increased during steaming and drying of cultivated ginseng, whereas it was more decreased during steaming and drying of CWG. 5. Ginsenoside Rb1 content was increased about 500% during steaming and drying of cultivated ginseng, whereas it was increased about 30% during steaming and drying of CWG, indicating that ginsenoside Rb1 was more generated in red ginseng than in RWG. 6. Ginsenoside Rg3 content was higher, whereas ginsenoside Rg1 content was lower in 11th RWG than in 9th RWG, indicating that ginsenoside Rg3 content was increased and Rg1 content was decreased as steaming and drying continued to proceed. Ginsenoside Rh2 and Rb1 contents began to be increased, followed by decreased after 9th steaming and drying process. Conclusions : Above experiment data can be an important indicator for the identification of ginseng, red ginseng, CWG, and RWG. And the following studies will be need for making good product using CWG.

  19. 基于改进农业生态区划法的耕地综合量能指数模型构建%Comprehensive index model building of cultivated land yield and productivity based on improved agro-ecological zoning method

    Institute of Scientific and Technical Information of China (English)

    陈丽; 郝晋珉; 艾东; 朱传民; 周宁; 洪舒蔓

    2014-01-01

    研究粮食产量与耕地产能间的关系以及区域差异,对于挖掘区域增产潜力,提高耕地资源利用效率,实现区域均衡增产,保障粮食安全战略具有重要意义。该文在农业生态区划法估算土地生产潜力基础上,提出基于耕地质量新内涵的耕地质量修正的耕地产能核算方法。通过探讨耕地综合产能的概念体系,构建了反映粮食产量与耕地产能关系的耕地综合量能指数模型,模型由耕地综合量能指数、耕地质量产能指数和耕地数量产能指数构成。并以曲周县为例,对该模型进行了实证研究,曲周县耕地综合量能指数为0.16,耕地质量产能指数为0.31,耕地数量产能指数为0.99,耕地总体产出效率不高,粮食增产的潜力较大,耕地粮食生产平均开发程度也不高,但数量增加潜力较小。%Study on the relationships between grain production and cultivated land productivity and its regional difference is of great significance for food security. Besides, it is also important for finding regional potential yields of grain, improving the utilization efficiency of cultivated land resources and achieving the regional increase of grain yield. In this paper, on the basis of agro-ecological zoning (AEZ) model, a new method was proposed to calculate the cultivated land productivity under cultivated land quality modification. Furthermore, the modification of cultivated land quality was based on a new concept that the quality of cultivated land was not only affected by natural factors but also by artificial factors such as irrigation canals, greenhouse, nursery factory, mechanical facilities and its supporting service station. By discussing the conceptual system of cultivated land integrated productivity which took the potential quantity of cultivated land and the quality index of cultivated land into the consideration, we proposed that comprehensive productivity of cultivated land

  20. Improved method for ex ovo-cultivation of developing chicken embryos for human stem cell xenografts.

    Science.gov (United States)

    Schomann, Timo; Qunneis, Firas; Widera, Darius; Kaltschmidt, Christian; Kaltschmidt, Barbara

    2013-01-01

    The characterization of human stem cells for the usability in regenerative medicine is particularly based on investigations regarding their differentiation potential in vivo. In this regard, the chicken embryo model represents an ideal model organism. However, the access to the chicken embryo is only achievable by windowing the eggshell resulting in limited visibility and accessibility in subsequent experiments. On the contrary, ex ovo-culture systems avoid such negative side effects. Here, we present an improved ex ovo-cultivation method enabling the embryos to survive 13 days in vitro. Optimized cultivation of chicken embryos resulted in a normal development regarding their size and weight. Our ex ovo-approach closely resembles the development of chicken embryos in ovo, as demonstrated by properly developed nervous system, bones, and cartilage at expected time points. Finally, we investigated the usability of our method for trans-species transplantation of adult stem cells by injecting human neural crest-derived stem cells into late Hamburger and Hamilton stages (HH26-HH28/E5-E6) of ex ovo-incubated embryos. We demonstrated the integration of human cells allowing experimentally easy investigation of the differentiation potential in the proper developmental context. Taken together, this ex ovo-method supports the prolonged cultivation of properly developing chicken embryos enabling integration studies of xenografted mammalian stem cells at late developmental stages. PMID:23554818

  1. Component Analysis of Cultivated Ginseng, Red Ginseng, Cultivated Wild Ginseng, and Red Wild Ginseng Using HPLC Method

    OpenAIRE

    Jang Ho, Lee; Ki Rok, Kwon; Bae Chun, Cha

    2008-01-01

    Objectives : The aim of this experiment is to provide an differentiation of ginseng, red ginseng, cultivated wild ginseng(CWG), and red wild ginseng(RWG) through component analysis using HPLC(High Performance Liquid Chromatography, hereafter HPLC). Methods : Comparative analyses of ginsenoside Rg3, ginsenoside Rh2, and ginsenosides Rb1 and Rg1 of various ginsengs were conducted using HPLC. Results : 1. CWG was relatively heat-resistant and showed slow change in color during the process ...

  2. To Cultivate Outstanding Software Engineer Based on Project-based Learning

    Institute of Scientific and Technical Information of China (English)

    LI Dong; MA Peiqun; LIU Shu

    2012-01-01

    This thesis sketches the connotation of project-based learning and introduces the basis on which project-based learning is practiced and applied in school of software as well as the plan of further practicing project-based learning. At the same time, this thesis also discusses application of project-based learning in "education and cultivation plan of excellent engineer".

  3. Effect of plant cultivation methods on content of major and trace elements in foodstuffs and retention in rats

    DEFF Research Database (Denmark)

    Kristensen, Mette; Østergaard, Lars F.; Halekoh, Ulrich;

    2008-01-01

    major and trace element contents of dried foodstuffs (carrots, kale, peas, potatoes and apples) grown in two consecutive years, as well as mineral retention determined in 36 rats (second generation in a multi-generation study) fed diets based on these foodstuffs from one year. RESULTS: Overall, there...... was no evident trend towards differences in element content of foodstuffs or diets due to the use of different cultivation systems, and differences between harvest years exceeded those seen between cultivation methods. Also, no significant differences in the retention of elements in rats fed diets...

  4. Application of gamma-ray transmission method for study the properties of cultivated soil

    International Nuclear Information System (INIS)

    Highlights: ► Gamma-ray transmission method and attenuation coefficients. ► Physical properties of cultivated soil. ► Soil–water systems management. - Abstract: The objective of the proposed work was to apply gamma-ray transmission method for studying the properties of cultivated soil. In this study, mass attenuation coefficients, bulk density, moisture content, porosity, and field capacity are determined. Five soil samples are collected from different agriculture zones in Egypt. A high-resolution gamma-ray spectrometry based on HPGe (high purity germanium) detector was employed to measure attenuation of strongly collimated monoenergetic gamma beam through soil samples. The radioactive sources used in the experiment were 241Am, 133Ba, 137Cs and 60Co. The mass attenuation coefficients of soil samples were calculated from the transmission measurements. WinXCom program was used to calculate mass attenuation coefficients of soil samples. There is a satisfactory agreement between experimental and theoretical values. It was observed that gamma-ray transmission method is an efficient tool for studying soil parameters for agriculture purposes.

  5. Potential of Homestay Tourism Based on Seaweed Cultivation from the Views of Seaweed Cultivators in District of Semporna Sabah, East Malaysia

    Directory of Open Access Journals (Sweden)

    Hussin Rosazman

    2014-01-01

    Full Text Available Community participation in tourism development especially among fisherman and farmers has begun to given serious attention by the government whereby the communities are given opportunities to engage in tourism development programmes in order to enhance their quality of life. In order to encourage local community participation in tourism development in rural areas, participants’ perceptions regarding tourism activities are important aspect to be sought. Good or bad perceptions from the community towards tourism development are important because it can determine the success of the programme. Firstly, this paper aims to explore the views or perceptions of seaweed cultivators towards homestay tourism which is based on seaweed cultivation in the District of Semporna, Sabah. Qualitative and quantitative research approaches have been applied in this study, such as the usage of the face to face interviews survey using survey questionnaires and field observation as primary methods. The findings show that the majority of the respondents have a positive perception of homestay tourism based on seaweed cultivation, such as the acceptance of visits by the tourists to their working place. Seaweed cultivators agreed that this tourism activity bring additional income to them. Activities that can become tourist attractions include tying seaweed seeds on a casino table. Moreover, the tourists have an opportunity to take a boat to see the seaweed farm, and take pictures of seaweed activities and so on. These findings also revealed that the majority of the respondents assumed that the visits of the tourists would motivate them to carry out the activity with more enthusiasm. This shows that seaweed cultivation could become a new tourism product which has great potential to develop in the district of Semporna, Sabah.

  6. 基于双边界二分式CVM的耕地社会价值评价——以重庆市为例%Appraisal of Cultivated Land Social Vaiue Based on Double-Bounded Contingent Valuation Method

    Institute of Scientific and Technical Information of China (English)

    唐建; 易乐庆; 彭珏

    2011-01-01

    以重庆市为例,采用双边界二分式CVM的logistic模型,从城镇居民支付意愿和农民受偿意愿2个角度考察耕地的社会价值.研究发现,WTP和WTA法下,耕地的社会价值巨大,且呈增长态势;城镇总体支付意愿和农民总体受偿意愿存在较大差距,目前仍需政府采取农业补贴等形式解决耕地外部性问题.%Taking the case of Chongqing, logistic model of double-bounded CVM is adopted, the social value of cultivated land are investigated from the angle of urban residents' willingness to pay and peasants' willingness to accept. The results show that there is great social value of cultivated land under WTP and WTA methods, and being a rising trend; there is a wide gap between urban WTP and rural WTA, and it still need the government to take agricultural subsidies to solve land externalities at present.

  7. Cultivation-based multiplex phenotyping of human gut microbiota allows targeted recovery of previously uncultured bacteria

    DEFF Research Database (Denmark)

    Rettedal, Elizabeth; Gumpert, Heidi; Sommer, Morten

    2014-01-01

    The human gut microbiota is linked to a variety of human health issues and implicated in antibiotic resistance gene dissemination. Most of these associations rely on culture-independent methods, since it is commonly believed that gut microbiota cannot be easily or sufficiently cultured. Here, we...... microbiota. Based on the phenotypic mapping, we tailor antibiotic combinations to specifically select for previously uncultivated bacteria. Utilizing this method we cultivate and sequence the genomes of four isolates, one of which apparently belongs to the genus Oscillibacter; uncultivated Oscillibacter...

  8. Appraisal of the Ecological Value of Cultivated Land Based on Double-Bounded Contingent Valuation Methods%基于双边界二分式CVM法的耕地生态价值评价——以重庆市为例

    Institute of Scientific and Technical Information of China (English)

    唐建; 沈田华; 彭珏

    2013-01-01

    There are externalities in the use of cultivated land, but there is no rational compensation for positive externality, and no effective restraint and containment for negative externality in China. Under the present system, fanners make use of their cultivated land for economic interests and ignore social and ecological opportunity costs. This pattern will lead to a reduction in cultivated land, quality degradation and environment pollution, and also seriously threaten food safety and sustainable agriculture. The ecological value of cultivated land should be evaluated objectively in order to reveal the extent of cultivated land ecological externalities. Research into the evaluation of the ecological value of cultivated land remains in the primary phase as there are many different methods with various results, most of which focus on urban WTP and ignore rural WTA. If WTA is ignored, it is difficult to build a rational compensation policy and incentive mechanisms for the ecological value of cultivated land. In order to solve this problem, views of urban WTP and rural WTA are taken into consideration. This paper uses Chongqing city as a focal city and applies logistic models of double-bounded contingent valuation methods to evaluate the ecological value of cultivated land. The results indicate that based on a 5% discount rate, the total ecological value of cultivated land in Chongqing is 87.6 billion CNY with WTP views and 213.2 billion CNY with WTA views. There is important ecological value under WTP and WTA method. There is a wide gap between urban WTP and rural WTA and therefore the government need to adopt measures to solve the ecological externalities of cultivated land, such as through agricultural subsidies.%评价耕地生态价值有利于了解耕地生态外部性大小,为政府制订合理的耕地补偿政策提供依据,实现耕地资源的可持续利用.本文以重庆市为例,采用双边界二分式CVM法的Logistic模型,从城镇居民的支付

  9. Curriculum-based library instruction from cultivating faculty relationships to assessment

    CERN Document Server

    Blevins, Amy

    2014-01-01

    Curriculum-Based Library Instruction: From Cultivating Faculty Relationships to Assessment highlights the movement beyond one-shot instruction sessions, specifically focusing on situations where academic librarians have developed curriculum based sessions and/or become involved in curriculum committees.

  10. Strategies for Cultivating New Peasants Based on Modern Agricultural Development

    Institute of Scientific and Technical Information of China (English)

    Fanxing; MENG; Jianguo; ZHOU

    2013-01-01

    Development of modern agriculture has higher and higher requirements on quality of peasants.This study,on the basis of connotations of modern agriculture,current situation and problems of Chinese peasants,and contradictions between modern agricultural development and peasants’ quality,specifies requirements of modern agriculture on new peasants,proposes corresponding suggestions to promote the cultivation of new Chinese peasants.

  11. Economic and energy assessment of minimalized soil tillage methods in maize cultivation

    Directory of Open Access Journals (Sweden)

    Piotr Szulc

    2014-06-01

    Full Text Available Grain yield of maize cultivated in the years 1997-2009 in monoculture and with annual tillage simplifications was assessed in energy and economy terms. Effects of no-tillage system and direct sowing (D with cultivation with autumn deep (A and shallow (B ploughing and cultivation with spring pre-sowing ploughing (C were compared. It was demonstrated that the 13-year maize grain yield in no-tillage system and direct sowing was lower by 10.4% than the yield obtained in conventional tillage system. The depth of autumn ploughing and its replacement with spring pre-sowing ploughing did not significantly affect the level of grain yield. Direct sowing compared to conventional tillage system reduced costs by on average 11.6%, and energy inputs only by 0.9%. The energy inputs of 100 kg grain production in direct sowing were higher by over 10% than the inputs of cultivations based on both autumn ploughing, irrespective of its depth, and of pre-sowing ploughing in the spring. The negative effect of direct sowing on grain yield was compensated by lower costs, which caused that the mean cost of 100 kg grain production for 13 years in direct sowing did not significantly differ from the costs of ploughing cultivations. Energy effectiveness of maize grain production in direct sowing was significantly lower than in the examined ploughing tillage cultivations. The soil tillage systems under study did not have a significant influence on economic effectiveness of maize cultivation evaluated with mean costs of production of 100 kg grain for 13 years.

  12. Quantification of encapsulated bioburden in spacecraft polymer materials by cultivation-dependent and molecular methods.

    Directory of Open Access Journals (Sweden)

    Anja Bauermeister

    Full Text Available Bioburden encapsulated in spacecraft polymers (such as adhesives and coatings poses a potential risk to jeopardize scientific exploration of other celestial bodies. This is particularly critical for spacecraft components intended for hard landing. So far, it remained unclear if polymers are indeed a source of microbial contamination. In addition, data with respect to survival of microbes during the embedding/polymerization process are sparse. In this study we developed testing strategies to quantitatively examine encapsulated bioburden in five different polymers used frequently and in large quantities on spaceflight hardware. As quantitative extraction of the bioburden from polymerized (solid materials did not prove feasible, contaminants were extracted from uncured precursors. Cultivation-based analyses revealed <0.1-2.5 colony forming units (cfu per cm3 polymer, whereas quantitative PCR-based detection of contaminants indicated considerably higher values, despite low DNA extraction efficiency. Results obtained from this approach reflect the most conservative proxy for encapsulated bioburden, as they give the maximum bioburden of the polymers irrespective of any additional physical and chemical stress occurring during polymerization. To address the latter issue, we deployed an embedding model to elucidate and monitor the physiological status of embedded Bacillus safensis spores in a cured polymer. Staining approaches using AlexaFluor succinimidyl ester 488 (AF488, propidium monoazide (PMA, CTC (5-cyano-2,3-diotolyl tetrazolium chloride demonstrated that embedded spores retained integrity, germination and cultivation ability even after polymerization of the adhesive Scotch-Weld 2216 B/A. Using the methods presented here, we were able to estimate the worst case contribution of encapsulated bioburden in different polymers to the bioburden of spacecraft. We demonstrated that spores were not affected by polymerization processes. Besides Planetary

  13. Construction of the Classification and Grading Index System of Cultivated Land Based on the Viewpoint of Sustainable Development

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    In order to objectively and reasonably evaluate the actual and potential value of cultivated land, both social and ecological values are introduced into the classification and grading index system of cultivated land based on the viewpoint of sustainable development, after considering the natural and economic values of cultivated land. Index system construction of the sustainable utilization of cultivated land should follow the principles of economic viability, social acceptability, and ecological protection. Classification of cultivated land should take into account the soil fertility of cultivated land. Then, grading of cultivated land is carried out from the practical productivity (or potential productivity) of cultivated land. According to the existing classification index system of cultivated land, the soil, natural and environmental factors in plains, mountains and hills are mainly modified in the classification index system of cultivated land. And index systems for the cultivated land classification in plains, mountains and hills are set up. The grading index system of cultivated land is established based on the economic viability (economic value), social acceptability (social value) and protection of cultivated land (ecological value). Quantitative expression of cultivated land grading index is also carried out.

  14. The Influence of Cultivation and Fertilization Methods on Yield of Eggplants Grown in a Polyethylene Greenhouse

    OpenAIRE

    Danut MANIUTIU; Rodica Maria SIMA

    2010-01-01

    In the experiment carried out in 2009 at University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania, the influence of cultivation and fertilization methods on yield of eggplants grown in polyethylene greenhouse was investigated. In the experiment, the early and total yields, as well as yield quality were determined. For the plants cultivated in organic substrate the yield reached about 92.9% in the case of early yield and about 58.6% in the case of total yield in compar...

  15. Collection and cultivation methods of Acartia tonsa for toxicity testing

    Energy Technology Data Exchange (ETDEWEB)

    Hood, C.A. [Baker Hughes INTEQ, Houston, TX (United States); Mayo, R.R. [ENSR Environmental Toxicology Lab., Houston, TX (United States)

    1995-12-31

    Acartia tonsa were located and collected from Galveston Bay, Texas in June 1995, using plankton nets and transported to the laboratory for culture. After literature searching and laboratory experimentation. A simple reliable method was designed to culture A. tonsa. This method requires a minimum of glassware and supplies. Adult A. tonsa are placed in one gallon bell jars filled with natural seawater. The jars are then maintained in a water bath at a constant temperature. Water changes are conducted twice weekly and organisms are fed daily with a mixture of algae, Skeletonema costatum, isocrysis galbana, and Thalassiosira sp. Gravid females are then isolated in generators for 24 hours to obtain known age neonates. The neonates are maintained up to a specific age and then are used in toxicity tests such as the ``Determination of the Acute Lethal Toxicity to Marine Copepods,`` required in the United Kingdom for all chemicals used for offshore drilling fluid applications.

  16. Collection and cultivation methods of Acartia tonsa for toxicity testing

    International Nuclear Information System (INIS)

    Acartia tonsa were located and collected from Galveston Bay, Texas in June 1995, using plankton nets and transported to the laboratory for culture. After literature searching and laboratory experimentation. A simple reliable method was designed to culture A. tonsa. This method requires a minimum of glassware and supplies. Adult A. tonsa are placed in one gallon bell jars filled with natural seawater. The jars are then maintained in a water bath at a constant temperature. Water changes are conducted twice weekly and organisms are fed daily with a mixture of algae, Skeletonema costatum, isocrysis galbana, and Thalassiosira sp. Gravid females are then isolated in generators for 24 hours to obtain known age neonates. The neonates are maintained up to a specific age and then are used in toxicity tests such as the ''Determination of the Acute Lethal Toxicity to Marine Copepods,'' required in the United Kingdom for all chemicals used for offshore drilling fluid applications

  17. Cultivating Life Skills at a Project-Based Charter School

    Science.gov (United States)

    Wurdinger, Scott; Enloe, Walter

    2011-01-01

    Surveys that focused on academic and life skill development were collected from alumni who attended Avalon Charter School in St Paul, Minnesota. Avalon is a small public charter school that uses project-based learning as their primary teaching method. Forty-two alumni responded to the online survey. Students ranked life skills such as creativity,…

  18. Crop based climate regimes for energy saving in greenhouse cultivation

    Energy Technology Data Exchange (ETDEWEB)

    Koerner, O.

    2003-06-16

    Sustainability is one of the major aims in greenhouse horticulture. According to agreements between the Dutch grower association and the government, energy consumption and the use of chemical biocides have to be reduced. More advanced greenhouse technique is being developed to reach the target to decrease the energy efficiency-index by 65% between 1980 and 2010. However, this could also be achieved with existing technology by using more advanced climate regimes. The present thesis aimed at that, through designing and analysing climate regimes while employing existing climate control possibilities. Theoretical temperature and humidity regimes were designed to decrease energy consumption and a photosynthesis maximisation procedure was implemented to maximise growth. The basis for a crop gross photosynthesis model for control purposes was created. Crop photosynthesis models were evaluated at conditions expected to occur with more sustainable climate regimes. It was shown with experimental evidence that theoretical assumptions on the temperature - CO2 effects in a crop that are based on theoretically models scaling up leaf photosynthesis to the crop level are valid and that simplified existing models could be applied up to 28C. With higher temperatures new designs are needed and this can probably be achieved with an improved stomata-resistance model. The well known temperature integration principle was modified with two nested time-frames (24-hour and six days) and a temperature dose-response function. In a year round tomato cultivation, energy consumption was predicted to decrease with up to 9 % compared to regular temperature integration. The potential for energy saving with temperature integration is limited by humidity control when as usual fixed set points are maintained, because it counteracts temperature integration. Vents open at lower temperatures and heating is switched on at higher temperatures than required for optimal effects of temperature integration. A

  19. Research Progress on Cultivation Methods of Microalgae%微藻培养方法研究进展

    Institute of Scientific and Technical Information of China (English)

    尚常花; 朱顺妮; 王忠铭; 袁振宏; 谢君

    2016-01-01

    微藻是一种有前景的生物柴油原料。微藻培养是微藻生物柴油生产过程的重要环节。本文就微藻培养方法的研究进展进行了阐述。对自养、异养及兼养三种培养方法进行了比较,并对微藻培养提出了建议。%Microalgae is a promising feedstock for biodiesel production. Cultivation of microalgae is a key step in the process of producing biodiesel using microalgae as raw material. Research progress on cultivation methods of microalgae is discussed. Phototrophic cultivation, heterotrophic cultivation and mixotrophic cultivation are compared. Suggestions about cultivation of microalgae are proposed in this article.

  20. Genetic Diversity Based on Allozyme Alleles of Chinese Cultivated Rice

    Institute of Scientific and Technical Information of China (English)

    TANG Sheng-xiang; WEI Xing-hua; JIANG Yun-zhu; D S Brar; G S Khush

    2007-01-01

    Genetic diversity was analyzed with 6 632 core rice cultivars selected from 60 282 Chinese rice accessions on the basis of 12 allozyme loci, Pgil, Pgi2, Ampl, Amp2, Amp3, Amp4, Sdh1, Adh1, Est1, Est2, Est5 and Est9, by starch gel electrophoresis. Among the materials examined, 52 alleles at 12 polymorphic loci were identified, which occupied 96.3% of 54 alleles found in cultivated germplasm of O.sativa L. The number of alleles per locus ranged from 2 to 7 with an average of 4.33. The gene diversity (He) each locus varied considerably from 0.017 for Amp4 to 0.583 for Est2 with an average gene diversity (Ht) 0.271, and Shannon-Wiener index from 0.055 to 0.946 with an average of 0.468. The degree of polymorphism (DP) was in a range from 0.9 to 46.9% with an average of 21.4%. It was found that the genetic diversity in japonica (Keng) subspecies was lower in terms of allele's number, Ht and S-W index, being 91.8, 66.2 and 75.7% of indica (Hsien) one, respectively. Significant genetic differentiation between indica and japonica rice has been appeared in the loci Pgil, Amp2, Pgi2, and Est2, with higher average coefficient of genetic differentiation (Gst) 0.635, 0.626, 0.322 and 0.282, respectively. Except less allele number per locus (3.33) for modern cultivars, being 76.9% of landraces, the Ht and S-W index showed in similar between the modern cultivars and the landraces detected. In terms of allozyme, the rice cultivars in the Southwest Plateau and Central China have richer genetic diversity. The present study reveals again that Chinese cultivated rice germplasm has rich genetic diversity, showed by the allozyme allele variation.

  1. Effect of planting methods and cyanobacterial inoculants on yield, water productivity and economics of rice cultivation

    Directory of Open Access Journals (Sweden)

    Amit A. Shahane

    2015-09-01

    Full Text Available The impact of two crop planting methods and of the application of cyanobacterial inoculants on plant growth, yield, water productivity and economics of rice cultivation was evaluated with the help of a split plot designed experiment during the rainy season of 2011 in New Delhi, India. Conventional transplanting and system of rice intensification (SRI were tested as two different planting methods and seven treatments that considered cyanobacterial inoculants and compost were applied with three repetitions each. Results revealed no significant differences in plant performance and crop yield between both planting methods. However, the application of biofilm based BGA bio-fertiliser + 2/3 N had an overall positive impact on both, plant performance (plant height, number of tillers and crop yield (number and weight of panicles as well as on grain and straw yield. Higher net return and a higher benefit-cost ratio were observed in rice fields under SRI planting method, whereas the application of BGA + PGPR + 2/3 N resulted in highest values. Total water productivity and irrigation water productivity was significantly higher under SRI practices (5.95 and 3.67 kg ha^(-1 mm^(-1 compared to practices of conventional transplanting (3.36 and 2.44, meaning that using SRI method, water saving of about 34 % could be achieved and significantly less water was required to produce one kg of rice. This study could show that a combination of plant growth promoting rhizobacteria (PGPR in conjunction with BGA and 2/3 dose of mineral N fertiliser can support crop growth performance, crop yields and reduces overall production cost, wherefore this practices should be used in the integrated nutrient management of rice fields in India.

  2. Diversity and habitat preferences of cultivated and uncultivated aerobic methanotrophic bacteria evaluated based on pmoA as molecular marker

    Directory of Open Access Journals (Sweden)

    Claudia eKnief

    2015-12-01

    Full Text Available Methane-oxidizing bacteria are characterized by their capability to grow on methane as sole source of carbon and energy. Cultivation-dependent and –independent methods have revealed that this functional guild of bacteria comprises a substantial diversity of organisms. In particular the use of cultivation-independent methods targeting a subunit of the particulate methane monooxygenase (pmoA as functional marker for the detection of aerobic methanotrophs has resulted in thousands of sequences representing unknown methanotrophic bacteria. This limits data interpretation due to restricted information about these uncultured methanotrophs. A few groups of uncultivated methanotrophs are assumed to play important roles in methane oxidation in specific habitats, while the biology behind other sequence clusters remains still largely unknown. The discovery of evolutionary related monooxygenases in non-methanotrophic bacteria and of pmoA paralogs in methanotrophs requires that sequence clusters of uncultivated organisms have to be interpreted with care. This review article describes the present diversity of cultivated and uncultivated aerobic methanotrophic bacteria based on pmoA gene sequence diversity. It summarizes current knowledge about cultivated and major clusters of uncultivated methanotrophic bacteria and evaluates habitat specificity of these bacteria at different levels of taxonomic resolution. Habitat specificity exists for diverse lineages and at different taxonomic levels. Methanotrophic genera such as Methylocystis and Methylocaldum are identified as generalists, but they harbor habitat specific methanotrophs at species level. This finding implies that future studies should consider these diverging preferences at different taxonomic levels when analyzing methanotrophic communities.

  3. Diversity and Habitat Preferences of Cultivated and Uncultivated Aerobic Methanotrophic Bacteria Evaluated Based on pmoA as Molecular Marker

    Science.gov (United States)

    Knief, Claudia

    2015-01-01

    Methane-oxidizing bacteria are characterized by their capability to grow on methane as sole source of carbon and energy. Cultivation-dependent and -independent methods have revealed that this functional guild of bacteria comprises a substantial diversity of organisms. In particular the use of cultivation-independent methods targeting a subunit of the particulate methane monooxygenase (pmoA) as functional marker for the detection of aerobic methanotrophs has resulted in thousands of sequences representing “unknown methanotrophic bacteria.” This limits data interpretation due to restricted information about these uncultured methanotrophs. A few groups of uncultivated methanotrophs are assumed to play important roles in methane oxidation in specific habitats, while the biology behind other sequence clusters remains still largely unknown. The discovery of evolutionary related monooxygenases in non-methanotrophic bacteria and of pmoA paralogs in methanotrophs requires that sequence clusters of uncultivated organisms have to be interpreted with care. This review article describes the present diversity of cultivated and uncultivated aerobic methanotrophic bacteria based on pmoA gene sequence diversity. It summarizes current knowledge about cultivated and major clusters of uncultivated methanotrophic bacteria and evaluates habitat specificity of these bacteria at different levels of taxonomic resolution. Habitat specificity exists for diverse lineages and at different taxonomic levels. Methanotrophic genera such as Methylocystis and Methylocaldum are identified as generalists, but they harbor habitat specific methanotrophs at species level. This finding implies that future studies should consider these diverging preferences at different taxonomic levels when analyzing methanotrophic communities. PMID:26696968

  4. Seasonal cultivated and fallow cropland mapping using MODIS-based automated cropland classification algorithm

    Science.gov (United States)

    Wu, Zhuoting; Thenkabail, Prasad S.; Mueller, Rick; Zakzeski, Audra; Melton, Forrest; Johnson, Lee; Rosevelt, Carolyn; Dwyer, John; Jones, Jeanine; Verdin, James P.

    2014-01-01

    Increasing drought occurrences and growing populations demand accurate, routine, and consistent cultivated and fallow cropland products to enable water and food security analysis. The overarching goal of this research was to develop and test automated cropland classification algorithm (ACCA) that provide accurate, consistent, and repeatable information on seasonal cultivated as well as seasonal fallow cropland extents and areas based on the Moderate Resolution Imaging Spectroradiometer remote sensing data. Seasonal ACCA development process involves writing series of iterative decision tree codes to separate cultivated and fallow croplands from noncroplands, aiming to accurately mirror reliable reference data sources. A pixel-by-pixel accuracy assessment when compared with the U.S. Department of Agriculture (USDA) cropland data showed, on average, a producer's accuracy of 93% and a user's accuracy of 85% across all months. Further, ACCA-derived cropland maps agreed well with the USDA Farm Service Agency crop acreage-reported data for both cultivated and fallow croplands with R-square values over 0.7 and field surveys with an accuracy of ≥95% for cultivated croplands and ≥76% for fallow croplands. Our results demonstrated the ability of ACCA to generate cropland products, such as cultivated and fallow cropland extents and areas, accurately, automatically, and repeatedly throughout the growing season.

  5. Seasonal cultivated and fallow cropland mapping using MODIS-based automated cropland classification algorithm

    Science.gov (United States)

    Wu, Zhuoting; Thenkabail, Prasad S.; Mueller, Rick; Zakzeski, Audra; Melton, Forrest; Johnson, Lee; Rosevelt, Carolyn; Dwyer, John; Jones, Jeanine; Verdin, James P.

    2013-01-01

    Increasing drought occurrences and growing populations demand accurate, routine, and consistent cultivated and fallow cropland products to enable water and food security analysis. The overarching goal of this research was to develop and test automated cropland classification algorithm (ACCA) that provide accurate, consistent, and repeatable information on seasonal cultivated as well as seasonal fallow cropland extents and areas based on the Moderate Resolution Imaging Spectroradiometer remote sensing data. Seasonal ACCA development process involves writing series of iterative decision tree codes to separate cultivated and fallow croplands from noncroplands, aiming to accurately mirror reliable reference data sources. A pixel-by-pixel accuracy assessment when compared with the U.S. Department of Agriculture (USDA) cropland data showed, on average, a producer’s accuracy of 93% and a user’s accuracy of 85% across all months. Further, ACCA-derived cropland maps agreed well with the USDA Farm Service Agency crop acreage-reported data for both cultivated and fallow croplands with R-square values over 0.7 and field surveys with an accuracy of ≥95% for cultivated croplands and ≥76% for fallow croplands. Our results demonstrated the ability of ACCA to generate cropland products, such as cultivated and fallow cropland extents and areas, accurately, automatically, and repeatedly throughout the growing season.

  6. Estimation of the sugar cane cultivated area from LANDSAT images using the two phase sampling method

    Science.gov (United States)

    Parada, N. D. J. (Principal Investigator); Cappelletti, C. A.; Mendonca, F. J.; Lee, D. C. L.; Shimabukuro, Y. E.

    1982-01-01

    A two phase sampling method and the optimal sampling segment dimensions for the estimation of sugar cane cultivated area were developed. This technique employs visual interpretations of LANDSAT images and panchromatic aerial photographs considered as the ground truth. The estimates, as a mean value of 100 simulated samples, represent 99.3% of the true value with a CV of approximately 1%; the relative efficiency of the two phase design was 157% when compared with a one phase aerial photographs sample.

  7. 一种分离培养硫酸盐还原菌的改进方法%A IMPROVED METHOD FOR ISLATION AND CULTIVATION OF SULFATE-REDUCING BACTERIA

    Institute of Scientific and Technical Information of China (English)

    万海清; 苏仕军; 葛长海; 万雪松; 朱家骅

    2003-01-01

    On the base of the characteristics of sulfate-reducing bacteria (SRB) growth and dish cultivated method, a new method for isolation and cultivation of SRB is established. It is dilution spread and repeat dish sandwish cultivated method with the exellence of dilution spread and embeded growth, and it can help separation and identification of SRB. The method has simple operation and less equipment. And using this method, the natural SRB drop was successfully gained, and small agar pieces containing SRB drop were acquired in easiness. The brief SRB transfering operation in strict anaerobic condition was in-deed realized. Fig 1, Ref 7.

  8. Diversity of Cultivable Methane-Oxidizing Bacteria in Microsites of a Rice Paddy Field: Investigation by Cultivation Method and Fluorescence in situ Hybridization (FISH)

    OpenAIRE

    Dianou, Dayéri; Ueno, Chihoko; Ogiso, Takuya; Kimura, Makoto; Asakawa, Susumu

    2012-01-01

    The diversity of cultivable methane-oxidizing bacteria (MOB) in the rice paddy field ecosystem was investigated by combined culture-dependent and fluorescence in situ hybridization (FISH) techniques. Seven microsites of a Japanese rice paddy field were the focus of the study: floodwater, surface soil, bulk soil, rhizosphere soil, root, basal stem of rice plant, and rice stumps of previous harvest. Based on pmoA gene analysis and transmission electron microscopy (TEM), four type I, and nine ty...

  9. Cultivation of microalgae in a tubular photobioreactor based on solar concentrators: distribution of light

    Czech Academy of Sciences Publication Activity Database

    Sergejevová, M.; Zaťková, I.; Červený, J.; Zrotalová, Kateřina; Masojídek, Jiří

    Brno, 2005, s. 65-65. [Biophysical and Biochemical Methods in Photosynthesis Research Central-European Conference. Brno (CZ), 15.09.2005-16.09.2005] R&D Projects: GA ČR GP522/05/P276 Institutional research plan: CEZ:AV0Z50200510 Keywords : microalga * cultivation Subject RIV: EE - Microbiology, Virology

  10. Genetic structure of cultivated flax (Linum usitatissimum L. based on retrotransposon-based markers

    Directory of Open Access Journals (Sweden)

    Habibollahi Hadi

    2015-01-01

    Full Text Available Flax (Linum usitatissimum L. is one of the most important fiber and oil crop plants cultivated since ancient time. The flax seeds contain high amount of omega- 3-fatty acids and biologically active lignans. In spite of economic importance of cultivated flax, no information is available on its genetic variability and population structure in Iran. Therefore, we used six inter-retrotransposon amplified polymorphism (IRAP markers and 15 combined IRAP markers to reveal within and among population genetic diversity in this crop plant. We used 30 randomly selected plants in three geographical populations for present investigation. AMOVA test produced significant genetic difference (PhiPT = 0.40, P = 0.010 among the studied populations and also revealed that, 40% of total genetic variability was due to within population diversity while, 60% was due to among population genetic differentiation. Gst (0.78, P = 0.001, Hedrick, standardised fixation index (G'st = 0.83, P = 0.001, revealed that the studied populations are genetically differentiated. STRUCTURE plot based on admixture model revealed that the studied populations differed extensively in their genetic content, but some degree of shared alleles occurred between them. Some adaptive IRAP loci were identified by LFMM analysis. These loci were private alleles restricted to geographical populations. Data obtained may be used in breeding and hybridization program of flax in the country.

  11. On the Cultivation of Automation Majors' Research Innovation Ability Based on Scientific Research Projects

    Science.gov (United States)

    Wang, Lipeng; Li, Mingqiu

    2012-01-01

    Currently, it has become a fundamental goal for the engineering major to cultivate high-quality engineering technicians with innovation ability in scientific research which is an important academic ability necessary for them. This paper mainly explores the development of comprehensive and designing experiments in automation based on scientific…

  12. Work-Based Research Degrees: Systematic Cultivation through a University-Industry Network Space

    Science.gov (United States)

    Sense, Andrew J.

    2016-01-01

    The purpose of this paper is to expound on an innovative approach to cultivating work-based Doctorates and Masters of Philosophy degrees, which involves close collaboration between industry organisations and a tertiary institution. This paper also reports on an examination of the programme's structural ability to help develop the learning…

  13. Cultivation-independent methods reveal differences among bacterial gut microbiota in triatomine vectors of Chagas disease.

    Directory of Open Access Journals (Sweden)

    Fabio Faria da Mota

    Full Text Available BACKGROUND: Chagas disease is a trypanosomiasis whose agent is the protozoan parasite Trypanosoma cruzi, which is transmitted to humans by hematophagous bugs known as triatomines. Even though insecticide treatments allow effective control of these bugs in most Latin American countries where Chagas disease is endemic, the disease still affects a large proportion of the population of South America. The features of the disease in humans have been extensively studied, and the genome of the parasite has been sequenced, but no effective drug is yet available to treat Chagas disease. The digestive tract of the insect vectors in which T. cruzi develops has been much less well investigated than blood from its human hosts and constitutes a dynamic environment with very different conditions. Thus, we investigated the composition of the predominant bacterial species of the microbiota in insect vectors from Rhodnius, Triatoma, Panstrongylus and Dipetalogaster genera. METHODOLOGY/PRINCIPAL FINDINGS: Microbiota of triatomine guts were investigated using cultivation-independent methods, i.e., phylogenetic analysis of 16s rDNA using denaturing gradient gel electrophoresis (DGGE and cloned-based sequencing. The Chao index showed that the diversity of bacterial species in triatomine guts is low, comprising fewer than 20 predominant species, and that these species vary between insect species. The analyses showed that Serratia predominates in Rhodnius, Arsenophonus predominates in Triatoma and Panstrongylus, while Candidatus Rohrkolberia predominates in Dipetalogaster. CONCLUSIONS/SIGNIFICANCE: The microbiota of triatomine guts represents one of the factors that may interfere with T. cruzi transmission and virulence in humans. The knowledge of its composition according to insect species is important for designing measures of biological control for T. cruzi. We found that the predominant species of the bacterial microbiota in triatomines form a group of low

  14. Crossing Methods and Cultivation Conditions for Rapid Production of Segregating Populations in Three Grain Amaranth Species.

    Science.gov (United States)

    Stetter, Markus G; Zeitler, Leo; Steinhaus, Adrian; Kroener, Karoline; Biljecki, Michelle; Schmid, Karl J

    2016-01-01

    Grain amaranths (Amaranthus spp.) have been cultivated for thousands of years in Central and South America. Their grains are of high nutritional value, but the low yield needs to be increased by selection of superior genotypes from genetically diverse breeding populations. Amaranths are adapted to harsh conditions and can be cultivated on marginal lands although little is known about their physiology. The development of controlled growing conditions and efficient crossing methods is important for research on and improvement of this ancient crop. Grain amaranth was domesticated in the Americas and is highly self-fertilizing with a large inflorescence consisting of thousands of very small flowers. We evaluated three different crossing methods (open pollination, hot water emasculation and hand emasculation) for their efficiency in amaranth and validated them with genetic markers. We identified cultivation conditions that allow an easy control of flowering time by day length manipulation and achieved flowering times of 4 weeks and generation times of 2 months. All three different crossing methods successfully produced hybrid F1 offspring, but with different success rates. Open pollination had the lowest (10%) and hand emasculation the highest success rate (74%). Hot water emasculation showed an intermediate success rate (26%) with a maximum of 94% success. It is simple to perform and suitable for a more large-scale production of hybrids. We further evaluated 11 single nucleotide polymorphism (SNP) markers and found that they were sufficient to validate all crosses of the genotypes used in this study for intra- and interspecific hybridizations. Despite its very small flowers, crosses in amaranth can be carried out efficiently and evaluated with inexpensive SNP markers. Suitable growth conditions strongly reduce the generation time and allow the control of plant height, flowering time, and seed production. In combination, this enables the rapid production of segregating

  15. Crossing methods and cultivation conditions for rapid production of segregating populations in three grain amaranth species

    Directory of Open Access Journals (Sweden)

    Markus G Stetter

    2016-06-01

    Full Text Available Grain amaranths (Amaranthus spp. have been cultivated for thousands of years in Central and South America. Their grains are of high nutritional value, but the low yield needs to be increased by selection of superior genotypes from genetically diverse breeding populations. Amaranths are adapted to harsh conditions and can be cultivated on marginal lands although little is known about their physiology. The development of controlled growing conditions and efficient crossing methods is important for research on and improvement of this ancient crop. Grain amaranth was domesticated in the Americas and is highly self-fertilizing with a large inflorescence consisting of thousands of very small flowers. We evaluated three different crossing methods (open pollination, hot water emasculation and hand emasculation for their efficiency in amaranth and validated them with genetic markers. We identified cultivation conditions that allow an easy control of flowering time by manipulating day length and achieved flowering times of four weeks and generation times of two months. All three different crossing methods successfully produced hybrid F1 offspring, but with different success rates. Open pollination had the lowest (10% and hand emasculation the highest success rate (74%. Hot water emasculation showed an intermediate success rate (26% with a maximum of 94% success. It is simple to perform and suitable for a more large-scale production of hybrids. We further evaluated 11 single nucleotide polymorphism (SNP markers and found that they were sufficient to validate all crosses of the genotypes used in this study for intra- and interspecific hybridisations. Despite its very small flowers, crosses in amaranth can be carried out efficiently and evaluated with inexpensive SNP markers. Suitable growth conditions strongly reduce the generation time and allow the control of plant height, flowering time and seed production. In combination, this enables the rapid

  16. Improved poliovirus D-antigen yields by application of different Vero cell cultivation methods.

    Science.gov (United States)

    Thomassen, Yvonne E; Rubingh, Olaf; Wijffels, René H; van der Pol, Leo A; Bakker, Wilfried A M

    2014-05-19

    Vero cells were grown adherent to microcarriers (Cytodex 1; 3 g L(-1)) using animal component free media in stirred-tank type bioreactors. Different strategies for media refreshment, daily media replacement (semi-batch), continuous media replacement (perfusion) and recirculation of media, were compared with batch cultivation. Cell densities increased using a feed strategy from 1×10(6) cells mL(-1) during batch cultivation to 1.8, 2.7 and 5.0×10(6) cells mL(-1) during semi-batch, perfusion and recirculation, respectively. The effects of these different cell culture strategies on subsequent poliovirus production were investigated. Increased cell densities allowed up to 3 times higher D-antigen levels when compared with that obtained from batch-wise Vero cell culture. However, the cell specific D-antigen production was lower when cells were infected at higher cell densities. This cell density effect is in good agreement with observations for different cell lines and virus types. From the evaluated alternative culture methods, application of a semi-batch mode of operations allowed the highest cell specific D-antigen production. The increased product yields that can easily be reached using these higher cell density cultivation methods, showed the possibility for better use of bioreactor capacity for the manufacturing of polio vaccines to ultimately reduce vaccine cost per dose. Further, the use of animal-component-free cell- and virus culture media shows opportunities for modernization of human viral vaccine manufacturing. PMID:24583004

  17. Paper-based device for separation and cultivation of single microalga.

    Science.gov (United States)

    Chen, Chih-Chung; Liu, Yi-Ju; Yao, Da-Jeng

    2015-12-01

    Single-cell separation is among the most useful techniques in biochemical research, diagnosis and various industrial applications. Microalgae species have great economic importance as industrial raw materials. Microalgae species collected from environment are typically a mixed and heterogeneous population of species that must be isolated and purified for examination and further application. Conventional methods, such as serial dilution and a streaking-plate method, are intensive of labor and inefficient. We developed a paper-based device for separation and cultivation of single microalga. The fabrication was simply conducted with a common laser printer and required only a few minutes without lithographic instruments and clean-room. The driving force of the paper device was simple capillarity without a complicated pump connection that is part of most devices for microfluidics. The open-structure design of the paper device makes it operable with a common laboratory micropipette for sample transfer and manipulation with a naked eye or adaptable to a robotic system with functionality of high-throughput retrieval and analysis. The efficiency of isolating a single cell from mixed microalgae species is seven times as great as with a conventional method involving serial dilution. The paper device can serve also as an incubator for microalgae growth on simply rinsing the paper with a growth medium. Many applications such as highly expressed cell selection and various single-cell analysis would be applicable. PMID:26459444

  18. On Cultivation Methods of College Student Laboratory Assistants Based on Self-managing Mode%基于自主管理模式的高校机房学生管理员培养方法研究

    Institute of Scientific and Technical Information of China (English)

    卢荣辉; 梁洪涛

    2011-01-01

    计算机机房是高校重要的实践教学场所,用户对机房的服务需求不断提高。通过自适应学习能力、团队协作能力的培养,建设了一支具有自我管理、自主学习能力的学生管理员队伍。学生管理员协助老师提高机房管理和维护效率的同时,其自身的计算机专业实践能力和综合素质得到了提高。%With computer laboratory becoming an important place of practical teaching in college,user's requirements of service provided have also increased greatly.Through the cultivation of their adaptive learning ability and team working ability,an efficient team of student lab assistants can be built who are good at self-management and self-learning.In this way,students can not only help teachers improve the efficiency of laboratory management and maintenance,but also improve their own practical abilities in computer and integrated qualities.

  19. Preferences based Control Design of Complex Fed-batch Cultivation Process

    Directory of Open Access Journals (Sweden)

    Yuri Pavlov

    2009-08-01

    Full Text Available In the paper is presented preferences based control design and stabilization of the growth rate of fed-batch cultivation processes. The control is based on an enlarged Wang-Monod-Yerusalimsky kinetic model. Expected utility theory is one of the approaches for utilization of conceptual information (expert preferences. In the article is discussed utilization of stochastic machine learning procedures for evaluation of expert utilities as criteria for optimization.

  20. Characteristics of Eastern Canadian cultivated Sphagnum and potential use as a substitute for perlite and vermiculite in peat-based horticultural substrates

    OpenAIRE

    M. Aubé; M. Quenum; L.L. Ranasinghe

    2015-01-01

    Sphagnum cultivation on harvested peatlands to meet wetland restoration objectives could be an economically feasible activity since cultivated Sphagnum has potential horticultural applications. We compared the characteristics of cultivated Sphagnum from Shippagan (Canada) with those of non-cultivated Sphagnum products from Chile, New Zealand and Canada, and assessed its potential as a perlite and vermiculite substitute in horticultural peat-based substrates. Shippagan cultivated Sphagnum was ...

  1. Support system based on GIS and weighted sum method for drawing up of land suitability map for agriculture. Application to durum wheat cultivation in the area of Mleta (Algeria)

    Energy Technology Data Exchange (ETDEWEB)

    Mendas, A.; Delali, A.

    2012-11-01

    Due to constant decrease in farmlands, it is important to identify the best lands for sustainable agriculture (productive and profitable agriculture that protects the environment and that is socially equitable). This requirement has resulted in the development of land suitability maps for agriculture by combining a range of factors. Spatial analysis approaches, based on the concepts of the weighted sum, combined with Geographical Information Systems (GIS) offer the opportunity to efficiently produce these land suitability maps. The functions of the weighted sum make it possible to assign numerical weights, to distinguish between positive and negative criteria and to rank alternatives. A spatial decision support system has been developed for establishing the land suitability map for agriculture. It incorporates a version of the weighted sum method SAW (Simple Additive Weighting), applicable to the vector data model, in ArcGIS within the GIS program package environment. This approach has been tested on the area of Mleta (Algeria) to assess the land suitability for durum wheat agriculture. The parameters and the classification system used in this work are inspired from the FAO. The coherence of results confirms the system effectiveness. (Author) 23 refs.

  2. Methods for the cultivation of ciliated protozoa from the large intestine of horses.

    Science.gov (United States)

    Bełżecki, Grzegorz; Miltko, Renata; Michałowski, Tadeusz; McEwan, Neil R

    2016-01-01

    This paper describes cultivation methods for ciliates from the digestive tract of horses. Members of three different genera were successfully grown in vitro for short periods of time. However, only cells belonging to the genus Blepharocorys, which resides in the horse's large intestine, were maintained for longer periods. This Blepharocorys culture was successfully grown in vitro after inoculation of freshly excreted horse faeces in culture medium containing a population of bacteria. The ciliates survived for over six months, and the density of their population varied between 1.7 × 10(3) and 2.4 × 10(3) cells mL(-1). Favourable conditions for the prolonged cultivation of this ciliate were observed when the medium was prepared by mixing horse faeces and 'caudatum' salt solution in a 1:1 V/V ratio together with food (60% powdered meadow hay, 16% wheat gluten, 12% barley flour and 12% microcrystalline cellulose) supplied as 0.20 mg mL(-1) culture per day. PMID:26684192

  3. A ddRAD Based Linkage Map of the Cultivated Strawberry, Fragaria xananassa

    OpenAIRE

    Davik, Jahn; Sargent, Daniel James; Brurberg, May Bente; Lien, Sigbjørn; Kent, Matthew; Alsheikh, Muath

    2015-01-01

    The cultivated strawberry (Fragaria ×ananassa Duch.) is an allo-octoploid considered difficult to disentangle genetically due to its four relatively similar sub-genomic chromosome sets. This has been alleviated by the recent release of the strawberry IStraw90 whole genome genotyping array. However, array resolution relies on the genotypes used in the array construction and may be of limited general use. SNP detection based on reduced genomic sequencing approaches has the potential of providin...

  4. Genetic structure of cultivated flax (Linum usitatissimum L.) based on retrotransposon-based markers

    OpenAIRE

    Habibollahi Hadi; Noormohammadi Zahra; Sheidai Masoud; Farahani Farah

    2015-01-01

    Flax (Linum usitatissimum L.) is one of the most important fiber and oil crop plants cultivated since ancient time. The flax seeds contain high amount of omega- 3-fatty acids and biologically active lignans. In spite of economic importance of cultivated flax, no information is available on its genetic variability and population structure in Iran. Therefore, we used six inter-retrotransposon amplified polymorphism (IRAP) markers and 15 combined IRAP markers ...

  5. Diversity and Habitat Preferences of Cultivated and Uncultivated Aerobic Methanotrophic Bacteria Evaluated Based on pmoA as Molecular Marker

    OpenAIRE

    Knief, Claudia

    2015-01-01

    Methane-oxidizing bacteria are characterized by their capability to grow on methane as sole source of carbon and energy. Cultivation-dependent and -independent methods have revealed that this functional guild of bacteria comprises a substantial diversity of organisms. In particular the use of cultivation-independent methods targeting a subunit of the particulate methane monooxygenase (pmoA) as functional marker for the detection of aerobic methanotrophs has resulted in thousands of sequences ...

  6. Online Supervision of Penicillin Cultivations Based on Rolling MPCA%基于滚动MPCA的青霉素发酵过程的在线监控

    Institute of Scientific and Technical Information of China (English)

    汪志锋; 袁景淇

    2007-01-01

    To reduce the variations of the production process in penicillin cultivations, a rolling multivariate statistical approach based on multiway principle component analysis (MPCA) is developed and used for fault diagnosis of penicillin cultivations. Using the moving data windows technique, the static MPCA is extended for use in dynamic process performance monitoring. The control chart is set up using the historical data collected from the past successful batches, thereby resulting in simplification of monitoring charts, easy tracking of the progress in each batch run, and monitoring the occurrence of the observable upsets. Data from the commercial-scale penicillin fermentation process are used to develop the rolling model. Using this method, faults are detected in real time and the corresponding measurements of these faults are directly made through inspection of a few simple plots (t-chart,SPE-chart, and T2-chart). Thus, the present methodology allows the process operator to actively monitor the data from several cultivations simultaneously.

  7. A Comparative Accuracy Analysis of Classification Methods in Determination of Cultivated Lands with Spot 5 Satellite Imagery

    Science.gov (United States)

    kaya, S.; Alganci, U.; Sertel, E.; Ustundag, B.

    2013-12-01

    A Comparative Accuracy Analysis of Classification Methods in Determination of Cultivated Lands with Spot 5 Satellite Imagery Ugur ALGANCI1, Sinasi KAYA1,2, Elif SERTEL1,2,Berk USTUNDAG3 1 ITU, Center for Satellite Communication and Remote Sensing, 34469, Maslak-Istanbul,Turkey 2 ITU, Department of Geomatics, 34469, Maslak-Istanbul, Turkey 3 ITU, Agricultural and Environmental Informatics Research Center,34469, Maslak-Istanbul,Turkey alganci@itu.edu.tr, kayasina@itu.edu.tr, sertele@itu.edu.tr, berk@berk.tc ABSTRACT Cultivated land determination and their area estimation are important tasks for agricultural management. Derived information is mostly used in agricultural policies and precision agriculture, in specifically; yield estimation, irrigation and fertilization management and farmers declaration verification etc. The use of satellite image in crop type identification and area estimate is common for two decades due to its capability of monitoring large areas, rapid data acquisition and spectral response to crop properties. With launch of high and very high spatial resolution optical satellites in the last decade, such kind of analysis have gained importance as they provide information at big scale. With increasing spatial resolution of satellite images, image classification methods to derive the information form them have become important with increase of the spectral heterogeneity within land objects. In this research, pixel based classification with maximum likelihood algorithm and object based classification with nearest neighbor algorithm were applied to 2012 dated 2.5 m resolution SPOT 5 satellite images in order to investigate the accuracy of these methods in determination of cotton and corn planted lands and their area estimation. Study area was selected in Sanliurfa Province located on Southeastern Turkey that contributes to Turkey's agricultural production in a major way. Classification results were compared in terms of crop type identification using

  8. UTILIZATION OF DNA MARKERS BASED ON MICROSATELLITE POLYMORPHISM FOR IDENTIFICATION OF POTATO VARIETIES CULTIVATED IN THE CZECH REPUBLIC

    Directory of Open Access Journals (Sweden)

    Alena Nováková

    2011-01-01

    Full Text Available In the year 2007, there were one hundred and seventy-eight potato varieties enlisted in the Czech list of registered potato varieties. The classical morphometric approach to characterization is not effective for such a number of varieties especially for identification at the level of tubers. The needfulness of variety identification at the level of tubers is important mainly for trade aspect. The Czech law no.110/1997 Sb. about the food-stuff and tobacco products and the consequential ordinance (MZe č. 332 / 1997 Sb. require guarantee of variety declaration in commercial relation for table potato. In this study we analyzed twenty potato varieties (Solanum tuberosum L. cultivated in the Czech Republic. Every variety was represented by four independent replicates. This set of samples was analyzed by methods of PCR-SSR (Simple Sequence Repeats and PCR-ISSR (Inter Simple Sequence Repeats. We discovered that both of tested methods afford sufficient polymorphism for variety identification, but the method of PCR-ISSR is not utilizable, because we observed the variability within variety. For outright identification of the whole set of potato varieties cultivated in the Czech Republic we recommend to use SSR, AFLP and retrotransposene-based markers as well as morphological markers.

  9. Development of a rule-based algorithm for rice cultivation mapping using Landsat 8 time series

    Science.gov (United States)

    Karydas, Christos G.; Toukiloglou, Pericles; Minakou, Chara; Gitas, Ioannis Z.

    2015-06-01

    In the framework of ERMES project (FP7 66983), an algorithm for mapping rice cultivation extents using mediumhigh resolution satellite data was developed. ERMES (An Earth obseRvation Model based RicE information Service) aims to develop a prototype of downstream service for rice yield modelling based on a combination of Earth Observation and in situ data. The algorithm was designed as a set of rules applied on a time series of Landsat 8 images, acquired throughout the rice cultivation season of 2014 from the plain of Thessaloniki, Greece. The rules rely on the use of spectral indices, such as the Normalized Difference Vegetation Index (NDVI), the Normalized Difference Water Index (NDWI), and the Normalized Seasonal Wetness Index (NSWI), extracted from the Landsat 8 dataset. The algorithm is subdivided into two phases: a) a hard classification phase, resulting in a binary map (rice/no-rice), where pixels are judged according to their performance in all the images of the time series, while index thresholds were defined after a trial and error approach; b) a soft classification phase, resulting in a fuzzy map, by assigning scores to the pixels which passed (as `rice') the first phase. Finally, a user-defined threshold of the fuzzy score will discriminate rice from no-rice pixels in the output map. The algorithm was tested in a subset of Thessaloniki plain against a set of selected field data. The results indicated an overall accuracy of the algorithm higher than 97%. The algorithm was also applied in a study are in Spain (Valencia) and a preliminary test indicated a similar performance, i.e. about 98%. Currently, the algorithm is being modified, so as to map rice extents early in the cultivation season (by the end of June), with a view to contribute more substantially to the rice yield prediction service of ERMES. Both algorithm modes (late and early) are planned to be tested in extra Mediterranean study areas, in Greece, Italy, and Spain.

  10. Genetic diversity of cultivated flax (Linum usitatissimum L.) germplasm assessed by retrotransposon-based markers.

    Science.gov (United States)

    Smýkal, P; Bačová-Kerteszová, N; Kalendar, R; Corander, J; Schulman, A H; Pavelek, M

    2011-05-01

    Retrotransposon segments were characterized and inter-retrotransposon amplified polymorphism (IRAP) markers developed for cultivated flax (Linum usitatissimum L.) and the Linum genus. Over 75 distinct long terminal repeat retrotransposon segments were cloned, the first set for Linum, and specific primers designed for them. IRAP was then used to evaluate genetic diversity among 708 accessions of cultivated flax comprising 143 landraces, 387 varieties, and 178 breeding lines. These included both traditional and modern, oil (86), fiber (351), and combined-use (271) accessions, originating from 36 countries, and 10 wild Linum species. The set of 10 most polymorphic primers yielded 141 reproducible informative data points per accession, with 52% polymorphism and a 0.34 Shannon diversity index. The maximal genetic diversity was detected among wild Linum species (100% IRAP polymorphism and 0.57 Jaccard similarity), while diversity within cultivated germplasm decreased from landraces (58%, 0.63) to breeding lines (48%, 0.85) and cultivars (50%, 0.81). Application of Bayesian methods for clustering resulted in the robust identification of 20 clusters of accessions, which were unstratified according to origin or user type. This indicates an overlap in genetic diversity despite disruptive selection for fiber versus oil types. Nevertheless, eight clusters contained high proportions (70-100%) of commercial cultivars, whereas two clusters were rich (60%) in landraces. These findings provide a basis for better flax germplasm management, core collection establishment, and exploration of diversity in breeding, as well as for exploration of the role of retrotransposons in flax genome dynamics. PMID:21293839

  11. Molecular identification based on ITS sequences for Kappaphycus and Eucheuma cultivated in China

    Institute of Scientific and Technical Information of China (English)

    ZHAO Sufen; HE Peimin

    2011-01-01

    The systematic classification of the Eucheurnatoideae is difficult because of their variable morphology and interpretation of reproductive structures.Kappaphycus and Eucheuma specimens cultivated on the Hainan and Fujian coast of China were introduced from Vietnam,the Philippines and Indonesia.Combined with morphological characteristics,all Kappaphycus and Eucheuma cultivated strains were identified by internal transcribed spacer (ITS) sequences.The phylogenetic tree was constructed using neighbor-joining and maximum likelihood methods.The results indicate that different ITS sequence lengths occurred in the different genera and species.An obvious difference in morphology could be found in the protuberance shape between Kappaphycus and Eucheuma.The protuberance in Eucheuma was thorn-like and in Kappaphycus was wartlike or papillate.Their ITS sequence lengths differed significantly in nucleotide variation rates up to 58.55%-63.90%.All nucleotide variations occurred in the ITS1 andITS2 regions except for five nucleotide transversions in the 5.8S rDNA region.In addition,the difference was at the branches among congeneric species.Kappaphycus sp.had branches with small buds,while K.alvarezii did not have such a feature.The nucleotide variation rates varied from 7.02% to 7.48% among species; within the same species of the clades it was <1.20%.Eucheumatoideae algae cultivated in China consisted of three clades,K.alvarezii,Kappaphycus sp.,and E.denticulatum.The results indicate that ITS sequence analysis was an effective way for identification of interspecies and intraspecies phylogenetic relationships and might provide a clue for molecular identification of algal Eucheumatoideae.

  12. Analysis of rhizosphere bacteria of rice cultivated in Andosol lowland and upland fields using molecular biological methods

    OpenAIRE

    DOI, Tetsuya; Hagiwara, Yusuke; Abe, Jun; Morita, Shigenori

    2007-01-01

    Bacteria in the rhizosphere influence plant growth and interact with plant roots. Microscopy and culture method have been used for studies of microorganisms of the rhizosphere, but these methods are insufficient for evaluation because most rhizosphere bacteria are viable but non-culturable (VBNC). Bacteria in the rhizosphere of rice cultivated in Andosol lowland and upland fields were analyzed in this study using PCR-DGGE and FISH, in combination with modified pretreatments. Results show that...

  13. High-power light-emitting diode based facility for plant cultivation

    Energy Technology Data Exchange (ETDEWEB)

    Tamulaitis, G [Institute of Materials Science and Applied Research, Vilnius University, Sauletekio al. 9-III, LT-10222 Vilnius (Lithuania); Duchovskis, P [Lithuanian Institute of Horticulture, Babtai, LT-54333 Kaunas District (Lithuania); Bliznikas, Z [Institute of Materials Science and Applied Research, Vilnius University, Sauletekio al. 9-III, LT-10222 Vilnius (Lithuania); Breive, K [Institute of Materials Science and Applied Research, Vilnius University, Sauletekio al. 9-III, LT-10222 Vilnius (Lithuania); Ulinskaite, R [Lithuanian Institute of Horticulture, Babtai, LT-54333 Kaunas District (Lithuania); Brazaityte, A [Lithuanian Institute of Horticulture, Babtai, LT-54333 Kaunas District (Lithuania); Novickovas, A [Institute of Materials Science and Applied Research, Vilnius University, Sauletekio al. 9-III, LT-10222 Vilnius (Lithuania); Zukauskas, A [Institute of Materials Science and Applied Research, Vilnius University, Sauletekio al. 9-III, LT-10222 Vilnius (Lithuania)

    2005-09-07

    Based on perspectives of the development of semiconductor materials systems for high-power light-emitting diodes (LEDs), an illumination facility for greenhouse plant cultivation was designed with the dominating 640 nm photosynthetically active component delivered by AlGaInP LEDs and supplementary components from AlGaN (photothropic action, 455 nm) and AlGaAs (photosynthetic 660 nm and photomorphogenetic 735 nm) LEDs. Photosynthesis intensity, photosynthetic productivity and growth morphology as well as chlorophyll and phytohormone concentrations were investigated in radish and lettuce grown in phytotron chambers under the LED-based illuminators and under high-pressure sodium (HPS) lamps with an equivalent photon flux density. Advantages of the high-power LED-based illuminators over conventional HPS lamps, applicability of AlGaInP LEDs for photosynthesis and control of plant growth by circadian manipulation of a relatively weak far-red component were demonstrated.

  14. High-power light-emitting diode based facility for plant cultivation

    International Nuclear Information System (INIS)

    Based on perspectives of the development of semiconductor materials systems for high-power light-emitting diodes (LEDs), an illumination facility for greenhouse plant cultivation was designed with the dominating 640 nm photosynthetically active component delivered by AlGaInP LEDs and supplementary components from AlGaN (photothropic action, 455 nm) and AlGaAs (photosynthetic 660 nm and photomorphogenetic 735 nm) LEDs. Photosynthesis intensity, photosynthetic productivity and growth morphology as well as chlorophyll and phytohormone concentrations were investigated in radish and lettuce grown in phytotron chambers under the LED-based illuminators and under high-pressure sodium (HPS) lamps with an equivalent photon flux density. Advantages of the high-power LED-based illuminators over conventional HPS lamps, applicability of AlGaInP LEDs for photosynthesis and control of plant growth by circadian manipulation of a relatively weak far-red component were demonstrated

  15. Estimation of Efficiency, Sustainability and Constraints in SRI (System of Rice Intensification) vis-a-vis Traditional Methods of Paddy Cultivation in North Coastal Zone of Andhra Pradesh

    OpenAIRE

    Rao, I.V.Y. Rama

    2011-01-01

    The study has assessed the economics and sustainability of SRI (system of rice intensification) and traditional methods of paddy cultivation in North Coastal Zone of Andhra Pradesh for the period 2008-09, based on the data of costs and returns of crop. Apart from budgeting techniques, benefit-cost ratio (BCR), yield gap analysis, sustainability index and response priority index have been employed in the study. It has shown that BCR is higher for SRI (1.76) than traditional (1.25) methods. Fur...

  16. Secretome data from Trichoderma reesei and Aspergillus niger cultivated in submerged and sequential fermentation methods.

    Science.gov (United States)

    Florencio, Camila; Cunha, Fernanda M; Badino, Alberto C; Farinas, Cristiane S; Ximenes, Eduardo; Ladisch, Michael R

    2016-09-01

    The cultivation procedure and the fungal strain applied for enzyme production may influence levels and profile of the proteins produced. The proteomic analysis data presented here provide critical information to compare proteins secreted by Trichoderma reesei and Aspergillus niger when cultivated through submerged and sequential fermentation processes, using steam-explosion sugarcane bagasse as inducer for enzyme production. The proteins were organized according to the families described in CAZy database as cellulases, hemicellulases, proteases/peptidases, cell-wall-protein, lipases, others (catalase, esterase, etc.), glycoside hydrolases families, predicted and hypothetical proteins. Further detailed analysis of this data is provided in "Secretome analysis of Trichoderma reesei and Aspergillus niger cultivated by submerged and sequential fermentation process: enzyme production for sugarcane bagasse hydrolysis" C. Florencio, F.M. Cunha, A.C Badino, C.S. Farinas, E. Ximenes, M.R. Ladisch (2016) [1]. PMID:27419196

  17. Can agricultural Cultivation Methods Influence the Healthfulness of Crops for Foods?

    DEFF Research Database (Denmark)

    Melballe Jensen, Maja; Jørgensen, Henry; Halekoh, Ulrich;

    2012-01-01

    . Additionally, the nutritional quality was affected by harvest year and location. However, harvest year and location rather than cultivation system affected the measured health biomarkers. In conclusion, the differences in dietary treatments composed of ingredients from different cultivation systems did not......The aim of the current study was to investigate if there are any health effects of long-term consumption of organically grown crops using a rat model. Crops were retrieved over two years from a long-term field trial at three different locations in Denmark, using three different cultivation systems...... lead to significant differences in the measured health biomarkers, except for a significant difference in plasma IgG levels....

  18. Cultivable endophytic bacteria from leaf bases of Agave tequilana and their role as plant growth promoters

    Directory of Open Access Journals (Sweden)

    Julia del C. Martínez-Rodríguez

    2014-12-01

    Full Text Available Agave tequilana Weber var. 'Azul' is grown for the production of tequila, inulin and syrup. Diverse bacteria inhabit plant tissues and play a crucial role for plant health and growth. In this study culturable endophytic bacteria were extracted from leaf bases of 100 healthy Agave tequilana plants. In plant tissue bacteria occurred at mean population densities of 3 million CFU/g of fresh plant tissue. Three hundred endophytic strains were isolated and 16s rDNA sequences grouped the bacteria into eight different taxa that shared high homology with other known sequences. Bacterial endophytes were identified as Acinectobacter sp., A. baumanii, A. bereziniae, Cronobacter sakazakii, Enterobacter hormaechei, Bacillus sp. Klebsiella oxytoca, Pseudomonas sp., Enterococcus casseliflavus, Leuconostoc mesenteroides subsp. mesenteroides and Gluconobacter oxydans. Isolates were confirmed to be plant growth promoting bacteria (PGPB by their capacities for nitrogen fixation, auxin production, phosphate solubilization, or antagonism against Fusarium oxysporum AC132. E. casseliflavus JM47 and K. oxytoca JM26 secreted the highest concentrations of IAA. The endophyte Acinectobacter sp. JM58 exhibited the maximum values for nitrogen fixation and phosphate solubilization index (PSI. Inhibition of fungi was found in Pseudomonas sp. JM9p and K. oxytoca JM26. Bacterial endophytes show promise for use as bio-inoculants for agave cultivation. Use of endophytes to enhance cultivation of agave may be particularly important for plants produced by micropropagation techniques, where native endophytes may have been lost.

  19. Cultivable endophytic bacteria from leaf bases of Agave tequilana and their role as plant growth promoters

    Science.gov (United States)

    Martínez-Rodríguez, Julia del C.; la Mora-Amutio, Marcela De; Plascencia-Correa, Luis A.; Audelo-Regalado, Esmeralda; Guardado, Francisco R.; Hernández-Sánchez, Elías; Peña-Ramírez, Yuri J.; Escalante, Adelfo; Beltrán-García, Miguel J.; Ogura, Tetsuya

    2014-01-01

    Agave tequilana Weber var. ‘Azul’ is grown for the production of tequila, inulin and syrup. Diverse bacteria inhabit plant tissues and play a crucial role for plant health and growth. In this study culturable endophytic bacteria were extracted from leaf bases of 100 healthy Agave tequilana plants. In plant tissue bacteria occurred at mean population densities of 3 million CFU/g of fresh plant tissue. Three hundred endophytic strains were isolated and 16s rDNA sequences grouped the bacteria into eight different taxa that shared high homology with other known sequences. Bacterial endophytes were identified as Acinectobacter sp., A. baumanii, A. bereziniae, Cronobacter sakazakii, Enterobacter hormaechei, Bacillus sp. Klebsiella oxytoca, Pseudomonas sp., Enterococcus casseliflavus, Leuconostoc mesenteroides subsp. mesenteroides and Gluconobacter oxydans. Isolates were confirmed to be plant growth promoting bacteria (PGPB) by their capacities for nitrogen fixation, auxin production, phosphate solubilization, or antagonism against Fusarium oxysporum AC132. E. casseliflavus JM47 and K. oxytoca JM26 secreted the highest concentrations of IAA. The endophyte Acinectobacter sp. JM58 exhibited the maximum values for nitrogen fixation and phosphate solubilization index (PSI). Inhibition of fungi was found in Pseudomonas sp. JM9p and K. oxytoca JM26. Bacterial endophytes show promise for use as bio-inoculants for agave cultivation. Use of endophytes to enhance cultivation of agave may be particularly important for plants produced by micropropagation techniques, where native endophytes may have been lost. PMID:25763038

  20. Simple and efficient method for isolation and cultivation of endoscopically obtained human colonocytes

    DEFF Research Database (Denmark)

    Seidelin, Jakob B; Horn, Thomas; Nielsen, Ole H

    2003-01-01

    and where the diagnosis of irritable bowel syndrome was later reached, were included. Seven colon biopsies were taken and incubated at varying time periods of 10-120 min and temperatures of 4-37 degrees C in a chelating buffer. The epithelium was then harvested and cultivated under three different...

  1. Model-based design of protected cultivation system - first results and remaining challenges

    OpenAIRE

    Henten, van, J.W.; Vanthoor, B.H.E.; Stanghellini, C.; Visser, de, P.H.B.; Hemming, S.

    2012-01-01

    Abstract: Protected cultivation systems are used throughout the world as a powerful instrument to produce crops. They protect the crops from unfavorable outdoor climate conditions and pests and offer the opportunity to modify the indoor climate to create an environment that is optimal for crop growth and production, both in terms of quality and quantity. A quick scan of protected cultivation systems presently in use reveals that quite a variety of protected cultivation systems can be found th...

  2. Comparison of Cheng's Index-and SSR Marker-based Classification of Asian Cultivated Rice

    Institute of Scientific and Technical Information of China (English)

    WANG Cai-hong; XU Qun; YU Ping; YUAN Xiao-ping; YU Han-yong; WANG Yi-ping; TANG Sheng-xiang

    2013-01-01

    A total of 100 cultivated rice accessions,with a clear isozyme-based classification,were analyzed based on Cheng's index and simple sequence repeat (SSR) marker.The results showed that the isozyme-based classification was in high accordance with that based on Cheng's index and SSR markers.Mantel-test revealed that the Euclidean distance of Cheng's index was significantly correlated with Nei's unbiased genetic distance of SSR markers (r =0.466,P ≤ 0.01).According to the model-based group and cluster analysis,the Cheng's index-and SSR-based classification coincided with each other,with the goodness of fit of 82.1% and 84.7% in indica,97.4% and 95.1% in japonica,respectively,showing higher accordance than that within subspecies.Therefore,Cheng's index could be used to classify subspecies,while SSR marker could be more efficient to analyze the subgroups within subspecies.

  3. Carotenoid profiling from 27 types of paprika (Capsicum annuum L.) with different colors, shapes, and cultivation methods.

    Science.gov (United States)

    Kim, Ji-Sun; An, Chul Geon; Park, Jong-Suk; Lim, Yong Pyo; Kim, Suna

    2016-06-15

    In this study, we investigated carotenoid profiles and contents from 27 types of paprika with different colors (red, orange, and yellow), shapes (blocky and conical), and cultivation methods (soil and soilless). We simultaneously analyzed 12 kinds of carotenoids using UPLC equipped with an HSS T3 column for 30 min, and we identified six kinds of carotenoids in red paprika and nine types in orange and yellow paprika. Zeaxanthin concentrations in orange paprika were in the range of 85.06±23.37-151.39±5.94 mg/100 g dry weight (dw), which shows that orange paprika is a great source of zeaxanthin. Generally, red paprika is a great source of capsanthin. However, a new cultivar, 'Mini Goggal Red', contained large amounts of zeaxanthin (121.41±30.10 mg/100 g dw) even though its visible color is red. This is very meaningful considering that consumers have a preference for red color and the potent functional value of zeaxanthin. Carotenoid profiles and concentrations in blocky and conical type paprika were not significantly different in red paprika except the 'Mini Goggal Red' cultivar and yellow paprika. Blocky type orange paprika contains plenty of zeaxanthin, unlike conical type orange paprika. Three new cultivars of the conical type were cultivated in both soil culture and soilless culture in the same province, and carotenoid profiles and concentrations were similar, showing that both cultivations methods can be used. PMID:26868549

  4. Mechanized methods for harvesting residual biomass from Mediterranean fruit tree cultivations

    OpenAIRE

    Borja Velázquez-Martí; Elena Fernández-González; Ángel Jesús Callejón-Ferre; Javier Estornell-Cremades

    2012-01-01

    This study evaluates the technology and work systems used in order to harvest residual biomass from pruning in the specific conditions of Mediterranean fruit orchards (narrow distances between crop-rows). Harvesting has been divided into several types of operations - pruning, biomass alignment between crop tracks, biomass concentration in piles, chipping and bundling - which have been analyzed in five Mediterranean cultivations for three years. Altogether, three types of pruning have been ana...

  5. The Production of High Purity Phycocyanin by Spirulina platensis Using Light-Emitting Diodes Based Two-Stage Cultivation.

    Science.gov (United States)

    Lee, Sang-Hyo; Lee, Ju Eun; Kim, Yoori; Lee, Seung-Yop

    2016-01-01

    Phycocyanin is a photosynthetic pigment found in photosynthetic cyanobacteria, cryptophytes, and red algae. In general, production of phycocyanin depends mainly on the light conditions during the cultivation period, and purification of phycocyanin requires expensive and complex procedures. In this study, we propose a new two-stage cultivation method to maximize the quantitative content and purity of phycocyanin obtained from Spirulina platensis using red and blue light-emitting diodes (LEDs) under different light intensities. In the first stage, Spirulina was cultured under a combination of red and blue LEDs to obtain the fast growth rate until reaching an absorbance of 1.4-1.6 at 680 nm. Next, blue LEDs were used to enhance the concentration and purity of the phycocyanin in Spirulina. Two weeks of the two-stage cultivation of Spirulina yielded 1.28 mg mL(-1) phycocyanin with the purity of 2.7 (OD620/OD280). PMID:26433600

  6. An influence of various soil cultivation methods in a combination with fertilizers on the radionuclide redistribution through the soil profile and the entering them into plants

    International Nuclear Information System (INIS)

    It is shown, that the radionuclide migration in agricultural landscapes significantly differs from virgin soils where the contamination (in 5 years after emergency) is concentrated in the upper turfy layer. Among agricultural methods the main factor, a changing radiation levels and the radioisotope content in the soil is a method its cultivation. As the cultivation depth increases the superficial gamma-backgroond and specific radionuclide content decreases, but contaminated layer radioactivity increases and the radionuclide migration intensity becomes stronger. With the regular plonghing at the same depth plough-foot effect occurs. It is expressed in increased contamination of the adjacent to the 'foot' soil layer. An alternation of cultivation methods and the cultivation depth is needed to destroy the plough-foot. 1 refs.; 4 tabs

  7. Improvement of erythromycin production by Saccharopolyspora erythraea in molasses based medium through cultivation medium optimization.

    Science.gov (United States)

    El-Enshasy, H A; Mohamed, N A; Farid, M A; El-Diwany, A I

    2008-07-01

    In the present work, erythromycin production was carried out in submerged culture using Saccharopolyspora erythraea. Different experiments were conducted to optimize the cultivation medium through the change of carbon and nitrogen sources to cheaper one in order to reduce the cost of medium and to utilize sugar cane molasses as one of major sugar industry by-products in Egypt. It was found that the addition of sugar cane molasses a sole carbon source at a concentration of 60 g/l accompanied by corn steep liquor (as organic N-source) in combination with ammonium sulphate (as inorganic N-source) gave the maximal erythromycin production. The antibiotic production in this medium reached about 600 mg/l which is about 33% higher than the value obtained in glucose based medium. On the other hand, the addition of n-propanol in concentration of 1% (v/v) increased the antibiotic production reaching about 720 mg/l after 144 h. Concluding, the new medium formulation based on cheap carbon source, sugar cane molasses, was a good alternative solution for the production of erythromycin economically. PMID:17936622

  8. Carbon and Nitrous Oxide Exchange from a Bioenergy Crop Cultivation on a Mineral Soil Measured with Eddy Covariance Method

    Science.gov (United States)

    Lind, S.; Shurpali, N.; Martikainen, P. J.

    2009-12-01

    Increased concentration of greenhouse gases (GHG; CO2, CH4, N2O) in the atmosphere have been linked to anthropogenic activities. Energy production based on fossil fuels is the main sources of CO2 to the atmosphere. Strategies to reduce CO2 emissions include use of bioenergy crops as energy source. To evaluate the real atmospheric value of bioenergy, knowledge on CO2 and N2O balances of ecosystems under bioenergy crop cultivation is needed. Reed canary grass (RCG, Phalaris arundinaceae L.) is increasingly being used as a bioenergy crop in the Nordic countries. It grows well in northern Europe as it tolerates flooding, requires short growing season and thrives well under low temperatures. Currently RCG is cultivated on an area of about 19000 ha in Finland and it is the highest yielding grass grown for energy purpose in Finland. The long rotation period (about ten years) of RCG cultivation without tilling favors carbon allocation into soil. First results from RCG cultivations on peat soil show that it is a promising land use option on such soils favoring soil carbon sequestration with low N2O emissions (Hyvönen et al. 2009; Shurpali et al. 2009). In order to devise a sound bioenergy policy based on this grass, studies on soils with variable characteristics under different climatic and hydrological regimes is urgently needed. Micrometeorological eddy covariance technique is suitable to study GHG fluxes at an ecosystem level (Baldocchi 2003). It is useful for continuous, direct and long-term measurements. Eddy covariance technique has been successfully used for measuring CO2 exchange in various ecosystems. The eddy covariance technique has also been used to measure N2O emissions (Denmead 2008). Aims of this study are to quantify the CO2 and N2O exchange of RCG cultivation on a mineral soil employing eddy covariance technique and to identify the key factors controlling the gas exchange. The study site on mineral soil is located in Eastern Finland. RCG was sown in spring

  9. A SSR-based composite genetic linkage map for the cultivated peanut (Arachis hypogaea L. genome

    Directory of Open Access Journals (Sweden)

    Li Shaoxiong

    2010-01-01

    Full Text Available Abstract Background The construction of genetic linkage maps for cultivated peanut (Arachis hypogaea L. has and continues to be an important research goal to facilitate quantitative trait locus (QTL analysis and gene tagging for use in a marker-assisted selection in breeding. Even though a few maps have been developed, they were constructed using diploid or interspecific tetraploid populations. The most recently published intra-specific map was constructed from the cross of cultivated peanuts, in which only 135 simple sequence repeat (SSR markers were sparsely populated in 22 linkage groups. The more detailed linkage map with sufficient markers is necessary to be feasible for QTL identification and marker-assisted selection. The objective of this study was to construct a genetic linkage map of cultivated peanut using simple sequence repeat (SSR markers derived primarily from peanut genomic sequences, expressed sequence tags (ESTs, and by "data mining" sequences released in GenBank. Results Three recombinant inbred lines (RILs populations were constructed from three crosses with one common female parental line Yueyou 13, a high yielding Spanish market type. The four parents were screened with 1044 primer pairs designed to amplify SSRs and 901 primer pairs produced clear PCR products. Of the 901 primer pairs, 146, 124 and 64 primer pairs (markers were polymorphic in these populations, respectively, and used in genotyping these RIL populations. Individual linkage maps were constructed from each of the three populations and a composite map based on 93 common loci were created using JoinMap. The composite linkage maps consist of 22 composite linkage groups (LG with 175 SSR markers (including 47 SSRs on the published AA genome maps, representing the 20 chromosomes of A. hypogaea. The total composite map length is 885.4 cM, with an average marker density of 5.8 cM. Segregation distortion in the 3 populations was 23.0%, 13.5% and 7.8% of the markers

  10. Calculating and testing cultivated land protection externality using Contingent Valuation Method%基于CVM的耕地保护外部性估算与检验

    Institute of Scientific and Technical Information of China (English)

    牛海鹏; 王文龙; 张安录

    2014-01-01

    This paper proposed the Contingent Valuation Method (CVM) for estimating cultivated land protection externality. The proposed CVM was based on reconstruction of cultivated land utilization benefit system and defined cultivated land protection externality. The paper discussed in detail specific techniques of sample size and distribution, determination of leading technology, pay/compensation interval setting, hypothetical market scenario setting, evasion of deviation, pretreatment of survey data, and results validity/reliability tests. The results showed that ecological and social benefits of cultivated lands included public goods with mixed classes across categories. These classes were provided by joint ventures between the pure positive externalities and the private sector (mainly farmers). This could be estimated by using CVM and other non-market valuation methods. The willingness to pay of inter-viewee for this service was heavily restricted and influenced by their levels of socioeconomic development. The recognition of eco-logical and social benefits of cultivated lands of farmers and urban residents formed the main causality factor for the differences in payment ratio between urban residents and rural farmers. Urban residents were more familiar with ecological and social benefits of cultivated lands than rural/farm households. This was probably a key factor influencing the willingness to pay for ecological services. Farm households preferred voluntary labor as mean of payment, while both money and voluntary labor were more preferred by urban residents. The probable reason for this was that farm households had more leisure time than urban residents. Unitary cultivated land protection externality for Jiaozuo City was 1.91×108 Yuan·a−1 and the cultivated land protection externality per unit area of cultivated land was 993.7 Yuan·hm−2·a−1 base on CVM. CVM validity and reliability test was a critical element of CVM theory, method and practice. This was

  11. Biodiversity of segetal weed community in continuous potato cultivated with metribuzin-based weed control

    OpenAIRE

    Pawlonka Zbigniew; Rymuza Katarzyna; Starczewski Krzysztof; Bombik Antoni

    2015-01-01

    The objective of the work reported here was to determine the relationship between herbicide rate and the biodiversity of weed communities in potato cultivated in continuous cropping. A seven-year field experiment was conducted to examine the effect of 4 metribuzin rates and an uncontrol on weed infestation in successive years of continuous potato cultivation. The following indices were calculated: the Shannon-Wiener and Simpson’s indices of species diversity and the Simpson’s index of dominat...

  12. A ddRAD Based Linkage Map of the Cultivated Strawberry, Fragaria xananassa.

    Science.gov (United States)

    Davik, Jahn; Sargent, Daniel James; Brurberg, May Bente; Lien, Sigbjørn; Kent, Matthew; Alsheikh, Muath

    2015-01-01

    The cultivated strawberry (Fragaria ×ananassa Duch.) is an allo-octoploid considered difficult to disentangle genetically due to its four relatively similar sub-genomic chromosome sets. This has been alleviated by the recent release of the strawberry IStraw90 whole genome genotyping array. However, array resolution relies on the genotypes used in the array construction and may be of limited general use. SNP detection based on reduced genomic sequencing approaches has the potential of providing better coverage in cases where the studied genotypes are only distantly related from the SNP array's construction foundation. Here we have used double digest restriction-associated DNA sequencing (ddRAD) to identify SNPs in a 145 seedling F1 hybrid population raised from the cross between the cultivars Sonata (♀) and Babette (♂). A linkage map containing 907 markers which spanned 1,581.5 cM across 31 linkage groups representing the 28 chromosomes of the species. Comparing the physical span of the SNP markers with the F. vesca genome sequence, the linkage groups resolved covered 79% of the estimated 830 Mb of the F. × ananassa genome. Here, we have developed the first linkage map for F. × ananassa using ddRAD and show that this technique and other related techniques are useful tools for linkage map development and downstream genetic studies in the octoploid strawberry. PMID:26398886

  13. Comparison of pooled standard deviation and standardized-t bootstrap methods for estimating uncertainty about average methane emission from rice cultivation

    Science.gov (United States)

    Kang, Namgoo; Jung, Min-Ho; Jeong, Hyun-Cheol; Lee, Yung-Seop

    2015-06-01

    The general sample standard deviation and the Monte-Carlo methods as an estimate of confidence interval is frequently being used for estimates of uncertainties with regard to greenhouse gas emission, based on the critical assumption that a given data set follows a normal (Gaussian) or statistically known probability distribution. However, uncertainty estimated using those methods are severely limited in practical applications where it is challenging to assume the probability distribution of a data set or where the real data distribution form appears to deviate significantly from statistically known probability distribution models. In order to solve these issues encountered especially in reasonable estimation of uncertainty about the average of greenhouse gas emission, we present two statistical methods, the pooled standard deviation method (PSDM) and the standardized-t bootstrap method (STBM) based upon statistical theories. We also report interesting results of the uncertainties about the average of a data set of methane (CH4) emission from rice cultivation under the four different irrigation conditions in Korea, measured by gas sampling and subsequent gas analysis. Results from the applications of the PSDM and the STBM to these rice cultivation methane emission data sets clearly demonstrate that the uncertainties estimated by the PSDM were significantly smaller than those by the STBM. We found that the PSDM needs to be adopted in many cases where a data probability distribution form appears to follow an assumed normal distribution with both spatial and temporal variations taken into account. However, the STBM is a more appropriate method widely applicable to practical situations where it is realistically impossible with the given data set to reasonably assume or determine a probability distribution model with a data set showing evidence of fairly asymmetric distribution but severely deviating from known probability distribution models.

  14. Development of test models to quantify encapsulated bioburden in spacecraft polymer materials by cultivation-dependent and molecular methods

    Science.gov (United States)

    Bauermeister, Anja; Moissl-Eichinger, Christine; Mahnert, Alexander; Probst, Alexander; Flier, Niwin; Auerbach, Anna; Weber, Christina; Haberer, Klaus; Boeker, Alexander

    Bioburden encapsulated in spacecraft polymers (such as adhesives and coatings) poses a potential risk to scientific exploration of other celestial bodies, but it is not easily detectable. In this study, we developed novel testing strategies to estimate the quantity of intrinsic encapsulated bioburden in polymers used frequently on spaceflight hardware. In particular Scotch-Weld (TM) 2216 B/A (Epoxy adhesive); MAP SG121FD (Silicone coating), Solithane (®) 113 (Urethane resin); ESP 495 (Silicone adhesive); and Dow Corning (®) 93-500 (Silicone encapsulant) were investigated. As extraction of bioburden from polymerized (solid) materials did not prove feasible, a method was devised to extract contaminants from uncured polymer precursors by dilution in organic solvents. Cultivation-dependent analyses showed less than 0.1-2.5 colony forming units (cfu) per cm³ polymer, whereas quantitative PCR with extracted DNA indicated considerably higher values, despite low DNA extraction efficiency. Results obtained by this method reflected the most conservative proxy for encapsulated bioburden. To observe the effect of physical and chemical stress occurring during polymerization on the viability of encapsulated contaminants, Bacillus safensis spores were embedded close to the surface in cured polymer, which facilitated access for different analytical techniques. Staining by AlexaFluor succinimidyl ester 488 (AF488), propidium monoazide (PMA), CTC (5-cyano-2,3-diotolyl tetrazolium chloride) and subsequent confocal laser scanning microscopy (CLSM) demonstrated that embedded spores retained integrity, germination and cultivation ability even after polymerization of the adhesive Scotch-Weld™ 2216 B/A.

  15. The Cultivation of Arabidopsis for Experimental Research Using Commercially Available Peat-Based and Peat-Free Growing Media.

    Directory of Open Access Journals (Sweden)

    Tiffany Drake

    Full Text Available Experimental research involving Arabidopsis thaliana often involves the quantification of phenotypic traits during cultivation on compost or other growing media. Many commercially-available growing media contain peat, but peat extraction is not sustainable due to its very slow rate of formation. Moreover, peat extraction reduces peatland biodiversity and releases stored carbon and methane into the atmosphere. Here, we compared the experimental performance of Arabidopsis on peat-based and several types of commercially-available peat-free growing media (variously formed from coir, composted bark, wood-fibre, and domestic compost, to provide guidance for reducing peat use in plant sciences research with Arabidopsis. Arabidopsis biomass accumulation and seed yield were reduced by cultivation on several types of peat-free growing media. Arabidopsis performed extremely poorly on coir alone, presumably because this medium was completely nitrate-free. Some peat-free growing media were more susceptible to fungal contamination. We found that autoclaving of control (peat-based growing media had no effect upon any physiological parameters that we examined, compared with non-autoclaved control growing media, under our experimental conditions. Overall, we conclude that Arabidopsis performs best when cultivated on peat-based growing media because seed yield was almost always reduced when peat-free media were used. This may be because standard laboratory protocols and growth conditions for Arabidopsis are optimized for peat-based media. However, during the vegetative growth phase several phenotypic traits were comparable between plants cultivated on peat-based and some peat-free media, suggesting that under certain circumstances peat-free media can be suitable for phenotypic analysis of Arabidopsis.

  16. The Effect of Cultivated Wild Ginseng Extract on Preadipocyte Proliferation

    Directory of Open Access Journals (Sweden)

    Byoung-Woo Kim

    2007-12-01

    Full Text Available Objectives : The purpose of this study is to investigate the effects of cultivated wild ginseng extract on primary cultured preadipocyte and adipocytes. Methods : Diminish preadipocyte proliferation does primary role to reduce obesity. So, preadipocytes and adipocytes were performed on cell cultures with using Sprague-Dawley rats and treated with 0.01-1mg/㎖ cultivated wild ginseng extract. Result : At all concentrations, cultivated wild ginseng extract wasn't show the suppress proliferation of preadipocytes significantly and failed to show effects on decomposition of adipocytes except high dosage. Conclusion : Based on these findings, cultivated wild ginseng is not a suitable choice for the treatment of localized obesity.

  17. Cultivation and utilization of specific wood biomass for synthesis of cellulose based bioethanol

    Energy Technology Data Exchange (ETDEWEB)

    Fara, L.; Comaneci, D. [Polytechnic Univ. of Bucharest, Bucharest (Romania). Faculty of Applied Sciences; Cincu, C.; Hubca, G.; Zaharia, C.; Diacon, A. [Polytechnic Univ. of Bucharest, Bucharest (Romania). Faculty of Applied Chemistry; Filat, M.; Chira, D. [Forest Research and Management Inst., Ilfov (Romania); Nutescu, C. [National Wood Inst., Bucharest (Romania); Fara, S. [Inst. for Research and Design of Automation, Bucharest (Romania)

    2010-07-01

    The energetic characteristics of 6 types of poplar clones cultivated for different pedoclimatic conditions in Romania were determined. Four clones were developed in Italy and 2 in Romania. Five experimental cultures were used to analyze the plant survival rate and biomass production rate. After 2 years of study, the Italian clones were found to have very good adaptability to the pedoclimatic conditions in Romania in comparison with local clones. The Italian clones Monviso and AF-6 registered the most substantial growths and the highest resistance to disease. Bioethanol was synthesized by acidic hydrolysis of the cellulose using 2 approaches. In the first approach the lignocellulosic raw material was hydrolyzed with diluted sulfuric acid at 50 degrees C for 24 hours. After filtration, the solid residue was treated with 30 per cent H{sub 2}SO{sub 4} at 100 degrees C for 6 hours. The resulting solutions were neutralized with Ca(OH){sub 2} following another filtration and the resulted solution with pH 6.5 was subjected to fermentation with Saccharomices Cerevisiae. In the second approach the lignocellulosic raw material was subjected to hydrolysis with 10 per cent H{sub 2}SO{sub 4} at 100 degrees C for 4 hours. After filtration, the solid residue was hydrolyzed with 30 per cent H{sub 2}SO{sub 4} at 100 degrees for 6 hours. The solution was neutralized with Ca(OH){sub 2} and subjected to alcoholic fermentation with Saccharomices Cerevisiae. The fermentation took place at 25 degrees C for 72 hours. The results for the two methods were similar.

  18. Research on Higher Vocational Student Professional Skill Quality Cultivation Methods%高职学生业务技能素质养成的方法研究

    Institute of Scientific and Technical Information of China (English)

    陈志强

    2015-01-01

    Aiming to the present situation of higher vocational student professional skill quality, it analyzes the research meaning of current higher vocational student features and its professional skill cultivation method. By the curriculum education cyber gamification, skill base training and professional skill manage-ment culture construction, it can work out the problems of higher vocational student profession skill quality cultivation.%针对高职学生的业务技能素质现况,分析目前高职学生的特点及其业务技能素质养成方法的研究意义;通过课程教育网络游戏化、技能基地训练和业务技能管理文化建设等途径,可以较好地解决高职学生业务技能素质养成的感知、熟知和精准问题。

  19. The influence of cultivation method on the flowering of Salvia horminum L. 'Oxford Blue'

    Directory of Open Access Journals (Sweden)

    Elżbieta Pogroszewska

    2012-12-01

    Full Text Available Salvia horminum L. 'Oxford Blue' was grown from transplants or it was direct seeded. Four sowing dates were applied: 15 March or 30 March - in a greenhouse, in order to obtain transplants, 13 April, 27 April and 11 May - sown directly into the ground. Three plant densities were applied: 25 plants×m2, 16 plants×m2, 12 plants×m2. It was found that Horminum sage can be grown from transplants or direct seeded. The cultivation from transplants is more advantageous due to the earlier flowering of plants, by about two weeks, and a better quality of inflorescences evaluated in terms of their length and size of bracteoles. Direct sowing of Horminum sage at two-week intervals from the 2nd decade of April till the 2nd decade of May ensures that by the end of August mature inflorescence stems are obtained, ready for cutting. A delay in the date of sowing results in the development of significantly shorter inflorescence stems, irrespective of the plant density. Plant density does not affect significantly the length of the period of inflorescence formation and the date of flowering, but a larger spacing is favourable to plants growing big, what results in a larger fresh weight of the above-ground portion. Sage grown from transplants responses to favourable climatic conditions by the development of bigger inflorescences.

  20. Mechanized methods for harvesting residual biomass from Mediterranean fruit tree cultivations

    Directory of Open Access Journals (Sweden)

    Borja Velázquez-Martí

    2012-06-01

    Full Text Available This study evaluates the technology and work systems used in order to harvest residual biomass from pruning in the specific conditions of Mediterranean fruit orchards (narrow distances between crop-rows. Harvesting has been divided into several types of operations - pruning, biomass alignment between crop tracks, biomass concentration in piles, chipping and bundling - which have been analyzed in five Mediterranean cultivations for three years. Altogether, three types of pruning have been analyzed: Manual, previous mechanical followed by manual, and fully mechanical; Two types of alignment: Manual and mechanical; Three concentration systems: Manual, tractor with a rake and a forwarder; Four chipping work organization systems: chipper driven inside orchard and manually fed by operators, mobile chipper driven inside orchard with pick-up header, mobile chipper fed by means of mechanical crane, chipper mounted on a truck fed by means of mechanical crane, which was working in a fixed position in a border of the plot after wood concentration. Also two bundling organization systems were checked: bundler machine working in a fixed position after wood concentration and working inside the plot driven among the crops. Previous concentration of the materials was the best alternative for their chipping or bundling in the studied conditions. Regression models have been calculated to predict the time of work of machinery and labor for each alternative. These equations were used to implement logistic planning as the Borvemar model, which defines a logistics network for supplying bio-energy systems.

  1. Biodiversity of segetal weed community in continuous potato cultivated with metribuzin-based weed control

    Directory of Open Access Journals (Sweden)

    Pawlonka Zbigniew

    2015-01-01

    Full Text Available The objective of the work reported here was to determine the relationship between herbicide rate and the biodiversity of weed communities in potato cultivated in continuous cropping. A seven-year field experiment was conducted to examine the effect of 4 metribuzin rates and an uncontrol on weed infestation in successive years of continuous potato cultivation. The following indices were calculated: the Shannon-Wiener and Simpson’s indices of species diversity and the Simpson’s index of domination. A total of 33 species were recorded in the experimental plots. Echinochoa crus-galli was the dominant species. The most abundant segetal communities were observed in untreated plots. An application of the herbicide reduced the biodiversity of the agrophytocenosis. Cultivation in continuous cropping increased the species number of the weed community in potato. The herbicide and cultivation in continuous cropping did not significantly affect the biodiversity indices but their values, to a great extent, confirmed the trends revealed by the analysis of weed infestation

  2. Influence of different cultivation methods on carbohydrate and lipid compositions and digestibility of energy of fruits and vegetables

    OpenAIRE

    Jørgensen, Henry; Knudsen, Knud Erik Bach; Lauridsen, Charlotte

    2012-01-01

    BACKGROUND Environmental as well as cultivation factors may greatly influence the chemical composition of plants. The main factors affecting the chemical composition of foodstuff are level and type of fertilizer (conventional and organic cultivation systems), location or soil type, and year of harvest. Organic foods are defined as products that are produced under controlled cultivation conditions characterized by the absence of synthetic fertilisers and very restricted use of pesticides. V...

  3. Self-Organizing Map and Multi-Layer Perceptron Neural Network Based Data Mining To Envisage Agriculture Cultivation

    Directory of Open Access Journals (Sweden)

    E. T. Venkatesh

    2008-01-01

    Full Text Available Study on characteristics of soil, to determine the types of crops suitable for cultivation in a particular region can increase the yield to greater extent, which minimizes the expenditures involved in irrigation and application of fertilizers. With the tested techniques available for calibrating the quality of soil and the crops suitable for cultivation in it, it is possible to determine the exact crop, irrigation patterns and even the cycle and quantity of fertilizer application. This paper dealt with the application of SOM based clustering and Artificial Intelligence techniques, to analyze the patterns of soils distributed across huge geographical area and identify the suitable types of crops for the particular soil. Estimation of exact crop(s suitable for a particular region can help stave off redundant maintenance and the inherent expenditures that would occur due to over irrigation and over usage of fertilizers, to fulfill the natural deficiencies. Our Focus is to improve the optimal utilization of innate characteristics in a soil through cultivation of appropriate crops, which will increase the volume and quality of yield, in particular for a developing country like India, where the huge majority of the population depends primarily on agriculture for livelihood.

  4. Effect of wastewater-borne bacteria on algal growth and nutrients removal in wastewater-based algae cultivation system.

    Science.gov (United States)

    Ma, Xiaochen; Zhou, Wenguang; Fu, Zongqiang; Cheng, Yanling; Min, Min; Liu, Yuhuan; Zhang, Yunkai; Chen, Paul; Ruan, Roger

    2014-09-01

    Centrate, a type of nutrient-rich municipal wastewater was used to determine the effect of wastewater-borne bacteria on algal growth and nutrients removal efficiency in this study. The characteristics of algal and bacterial growth profiles, wastewater nutrient removal and effect of initial algal inoculums were systematically examined. The results showed that initial algal concentration had apparent effect on bacterial growth, and the presence of bacteria had a significant influence on algal growth pattern, suggesting symbiotic relationship between algae and bacteria at the initial stage of algae cultivation. The maximum algal biomass of 2.01 g/L with 0.1g/L initial algal inoculums concentration can be obtained during algae cultivation in raw centrate medium. The synergistic effect of centrate-borne bacteria and microalgae on algae growth and nutrient removal performance at initial fast growth stage has great potential to be applied to pilot-scale wastewater-based algae wastewater system cultivated in continuous or semi-continuous mode. PMID:24968106

  5. Model based optimization of high cell density cultivation of nitrogen-fixing cyanobacteria.

    Science.gov (United States)

    Alagesan, Swathi; Gaudana, Sandeep B; Krishnakumar, S; Wangikar, Pramod P

    2013-11-01

    In the present study, fed-batch cultivation of Cyanothece sp. ATCC 51142, a known hydrogen producer, was optimized for maximizing biomass production. Decline in growth of this organism in dense cultures was attributed to increased substrate consumption for maintenance and respiration, and photolimitation due to self shading. A model incorporating these aspects was developed, and by using control vector parameterization (CVP), substrate feeding recipe was optimized to achieve 12-fold higher biomass concentration. The optimization results were verified experimentally on shake flask and bioreactor. The latter resulted in greater exponential growth rate possibly by overcoming photolimitation by simulating flashing light effect. Such a strategy can be readily applied for mixotrophic cultivation of cyanobacterial cultures in the first stage followed by photoautotrophic growth at the production stage. PMID:24047683

  6. Cultivating New-type Farmers Based on the Theory of Human Resources Development

    OpenAIRE

    Zhang, Li

    2010-01-01

    Under the direction of theory of human resources development, this thesis analyzes the impact of rural human resources development on cultivating new-type farmers. Firstly, it increases the input of rural basic education; secondly, it reinforces the vocational education and technology training; thirdly, it promotes the rural medical and public health services; fourthly, it quickens the rural labor transfer. The status quo of China’s rural human resources has been analyzed as follows: in ter...

  7. The Cultivation of Cluster’s Sustainable Competence Based on Knowledge Management

    OpenAIRE

    Tong Yang; Nengmin Wang

    2009-01-01

    The cluster has shown its powerful competitive advantages in global competition. However, as amounts of industrial clusters have displayed their competence, some have lost their competitive advantages in global competition. It is meaningful for clusters’ development to cultivate their sustainable competence. This paper analyzes the sources of cluster competence. According this paper, the important ways to make cluster competence sustainable include transferring and sharing knowledge, making i...

  8. Stainability of Cultivated Land in Henan Province Based on Ecological Footprint

    OpenAIRE

    Li, Ying-chao

    2009-01-01

    This paper summarizes the regional situation of Henan Province of China, and introduces the principle of ecological footprint mode, as well as computing formulas of ecological footprint and ecological capacity. According to the related data of Henan Province from 1998 to 2007, this paper firstly calculates the per capita ecological footprint and per capita ecological capacity of Henan Province. Result indicates that cultivated land in Henan Province shows ecological surplus with sustainable l...

  9. Study of Cultivating Intercultural Awareness in High School--Based on English Euphemism

    Institute of Scientific and Technical Information of China (English)

    孔曼羚

    2014-01-01

    Euphemism, which is one of universal language phenomena, provides an important approach to improve the commu-nicative effects and maintain interpersonal relationship harmoniously. This paper aims to arouse the attention of high school Eng-lish teachers to cultivate students’intercultural awareness. This thesis, with a brief introduction of English euphemism, presents a profound study of how to promote the intercultural awareness of students in high school with the help of English euphemism.

  10. Characteristics of Eastern Canadian cultivated Sphagnum and potential use as a substitute for perlite and vermiculite in peat-based horticultural substrates

    Directory of Open Access Journals (Sweden)

    M. Aubé

    2015-03-01

    Full Text Available Sphagnum cultivation on harvested peatlands to meet wetland restoration objectives could be an economically feasible activity since cultivated Sphagnum has potential horticultural applications. We compared the characteristics of cultivated Sphagnum from Shippagan (Canada with those of non-cultivated Sphagnum products from Chile, New Zealand and Canada, and assessed its potential as a perlite and vermiculite substitute in horticultural peat-based substrates. Shippagan cultivated Sphagnum was shorter than the Chilean and New Zealand products with which it was compared, yet more similar to them than to the Canadian product currently on the market. Laboratory tests on physical properties and greenhouse growth trials indicated that 50–100 % of the perlite or vermiculite of a peat-based substrate can be successfully replaced with cultivated Sphagnum. Non-sieved coarsely shredded Sphagnum or the large (> 6.3 mm fragments of sieved coarsely shredded Sphagnum best replicated the aeration provided by perlite and vermiculite in the substrates that were tested. Decomposition tests and comparisons of changes in physical properties of substrates containing Sphagnum after six weeks of growth trials indicated that Sphagnum degradation leading to reduced substrate performance is not likely to be an issue. Therefore, cultivated Sphagnum has great potential as a substitute for perlite and vermiculite.

  11. A one-stage cultivation process for lipid- and carbohydrate-rich biomass of Scenedesmus obtusiusculus based on artificial and natural water sources.

    Science.gov (United States)

    Schulze, Christian; Reinhardt, Jakob; Wurster, Martina; Ortiz-Tena, José Guillermo; Sieber, Volker; Mundt, Sabine

    2016-10-01

    A one-stage cultivation process of the microalgae Scenedesmus obtusiusculus with medium based on natural water sources was developed to enhance lipids and carbohydrates. A medium based on artificial sea water, Baltic Sea water and river water with optimized nutrient concentrations compared to the standard BG-11 for nitrate (-75%), phosphate and iron (-90%) was used for cultivation. Although nitrate exhaustion over cultivation resulted in nitrate limitation, growth of the microalgae was not reduced. The lipid content increased from 6.0% to 19.9%, an increase in oleic and stearic acid was observed. The unsaponifiable matter of the lipid fraction was reduced from 19.5% to 11.4%. The carbohydrate yield rose from 45% to 50% and the protein content decreased from 32.4% to 15.9%. Using natural water sources with optimized nutrient concentrations could open the opportunity to modulate biomass composition and to reduce the cultivation costs. PMID:27394996

  12. Analysis of 2-(2-Phenylethyl)chromones by UPLC-ESI-QTOF-MS and Multivariate Statistical Methods in Wild and Cultivated Agarwood

    Science.gov (United States)

    Li, Yuanbin; Sheng, Nan; Wang, Lingli; Li, Shijie; Chen, Jiannan; Lai, Xiaoping

    2016-01-01

    Agarwood is the fragrant resinous material mainly formed from species of Aquilaria. 2-(2-phenylethyl)chromones, especially the highly oxidized 5,6,7,8-tetrahydro-2-(2-phenylethyl)chromones, are the main representative compounds from agarwood. It is important to determine whether agarwood in trade is from cultivated trees or natural trees in the Convention on the International Trade in Endangered Species (CITES). We characterized the 2-(2-phenylethyl)chromones in agarwood by ultra-performance liquid chromatography coupled with electrospray ionization mass spectrometry (UPLC–ESI-QTOF-MS) and differentiated wild from cultivated agarwood by metabolomic analysis. A total of 141 chromones including 50 potentially new compounds were evaluated as belonging to four structural classes (unoxidized 2-(2-phenylethyl)chromones, 5,6,7,8-tetrahydro-2-(2-phenylethyl)-chromones, bi-2-(2-phenylethyl)chromones, and tri-2-(2-phenylethyl)chromones). The metabolic difference between wild and cultivated agarwood was analyzed by component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). Fourteen markers of metabolisms in wild and cultivated agarwood were constructed (e.g., 6,7-dimethoxy-2-(2-phenylethyl)chromone, 6,8-dihydroxy-2-(2-phenylethyl)chromone, 6-methoxy-2-(2-phenylethyl)chromone, etc.). These results indicated that UPLC–ESI-QTOF-MS-based metabonomics analysis in agarwood may be useful for distinguishing wild agarwood from cultivated agarwood. PMID:27223280

  13. Analysis of 2-(2-Phenylethyl)chromones by UPLC-ESI-QTOF-MS and Multivariate Statistical Methods in Wild and Cultivated Agarwood.

    Science.gov (United States)

    Li, Yuanbin; Sheng, Nan; Wang, Lingli; Li, Shijie; Chen, Jiannan; Lai, Xiaoping

    2016-01-01

    Agarwood is the fragrant resinous material mainly formed from species of Aquilaria. 2-(2-phenylethyl)chromones, especially the highly oxidized 5,6,7,8-tetrahydro-2-(2-phenylethyl)chromones, are the main representative compounds from agarwood. It is important to determine whether agarwood in trade is from cultivated trees or natural trees in the Convention on the International Trade in Endangered Species (CITES). We characterized the 2-(2-phenylethyl)chromones in agarwood by ultra-performance liquid chromatography coupled with electrospray ionization mass spectrometry (UPLC-ESI-QTOF-MS) and differentiated wild from cultivated agarwood by metabolomic analysis. A total of 141 chromones including 50 potentially new compounds were evaluated as belonging to four structural classes (unoxidized 2-(2-phenylethyl)chromones, 5,6,7,8-tetrahydro-2-(2-phenylethyl)-chromones, bi-2-(2-phenylethyl)chromones, and tri-2-(2-phenylethyl)chromones). The metabolic difference between wild and cultivated agarwood was analyzed by component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). Fourteen markers of metabolisms in wild and cultivated agarwood were constructed (e.g., 6,7-dimethoxy-2-(2-phenylethyl)chromone, 6,8-dihydroxy-2-(2-phenylethyl)chromone, 6-methoxy-2-(2-phenylethyl)chromone, etc.). These results indicated that UPLC-ESI-QTOF-MS-based metabonomics analysis in agarwood may be useful for distinguishing wild agarwood from cultivated agarwood. PMID:27223280

  14. Improved method for ex ovo-cultivation of developing chicken embryos for human stem cell xenografts

    OpenAIRE

    Timo Schomann; Firas Qunneis; Darius Widera; Christian Kaltschmidt; Barbara Kaltschmidt

    2013-01-01

    The characterization of human stem cells for the usability in regenerative medicine is particularly based on investigations regarding their differentiation potential in vivo. In this regard, the chicken embryo model represents an ideal model organism. However, the access to the chicken embryo is only achievable by windowing the eggshell resulting in limited visibility and accessibility in subsequent experiments. On the contrary, ex ovo-culture systems avoid such negative side effects. Her...

  15. Estimation of the stand ages of tropical secondary forests after shifting cultivation based on the combination of WorldView-2 and time-series Landsat images

    Science.gov (United States)

    Fujiki, Shogoro; Okada, Kei-ichi; Nishio, Shogo; Kitayama, Kanehiro

    2016-09-01

    We developed a new method to estimate stand ages of secondary vegetation in the Bornean montane zone, where local people conduct traditional shifting cultivation and protected areas are surrounded by patches of recovering secondary vegetation of various ages. Identifying stand ages at the landscape level is critical to improve conservation policies. We combined a high-resolution satellite image (WorldView-2) with time-series Landsat images. We extracted stand ages (the time elapsed since the most recent slash and burn) from a change-detection analysis with Landsat time-series images and superimposed the derived stand ages on the segments classified by object-based image analysis using WorldView-2. We regarded stand ages as a response variable, and object-based metrics as independent variables, to develop regression models that explain stand ages. Subsequently, we classified the vegetation of the target area into six age units and one rubber plantation unit (1-3 yr, 3-5 yr, 5-7 yr, 7-30 yr, 30-50 yr, >50 yr and 'rubber plantation') using regression models and linear discriminant analyses. Validation demonstrated an accuracy of 84.3%. Our approach is particularly effective in classifying highly dynamic pioneer vegetation younger than 7 years into 2-yr intervals, suggesting that rapid changes in vegetation canopies can be detected with high accuracy. The combination of a spectral time-series analysis and object-based metrics based on high-resolution imagery enabled the classification of dynamic vegetation under intensive shifting cultivation and yielded an informative land cover map based on stand ages.

  16. DNA evolution in cultivated plants

    Directory of Open Access Journals (Sweden)

    A. LAZANYI

    1987-08-01

    Full Text Available In long cultivated plant species - such as wheat, barley, bean, soya, tomato - during their microevolution from wild to modern cultivated species, the genome size has increased by 200 - 300 %. Presumably the newly acquired DNA of the cultivated species consists both of no coding, repetitive DNA and of new copies of the polygenesis that control the culture-characters. It seems, that in cultivated plants the genetic bases of the gigas-character and of the allometric-growth of the plant organs useful for man, are the amplified polygenesis, which control these characters.

  17. Influence of different cultivation methods on carbohydrate and lipid compositions and digestibility of energy of fruits and vegetables

    DEFF Research Database (Denmark)

    Jørgensen, Henry; Knudsen, Knud Erik Bach; Lauridsen, Charlotte

    2012-01-01

    of carbohydrates and fatty acids of fruits and vegetables. The objective was to investigate the influence of organic and conventional cultivation systems on the carbohydrate and fatty acid composition and digestibility of the energy of apple, carrot, kale, pea, potato, and rape seed oil. RESULTS: Carbohydrate......BACKGROUND: Environmental as well as cultivation factors may greatly influence the chemical composition of plants. The main factors affecting the chemical composition of foodstuff are level and type of fertilizer (conventional and organic cultivation systems), location or soil type, and year...... and lignin values ranged from 584 g kg−1 dry matter in kale to 910 g kg−1 DM in potato, but with significant differences in the proportion of sugars, starch, non-starch polysaccharides, and lignin between the foodstuffs. Triacylglycerol was the major lipid class in pea, with 82% of total fatty acids...

  18. A model based method for evaluation of crop operation scenarios in greenhouses

    NARCIS (Netherlands)

    Ooster, van 't A.

    2015-01-01

    Abstract This research initiated a model-based method to analyse labour in crop production systems and to quantify effects of system changes in order to contribute to effective greenhouse crop cultivation systems with efficient use of human labour and technology. This method was gra

  19. Evaluation on Fuzzy Variable Weight of Cultivated Land Fertility Based on GIS

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    [Objective] The modified variable weights based on constant weight and in- troduced theory of equalization function would better incorporate authentic index weights and make evaluation results of fertility more scientific. [Method] In Gaozhou City, the final weights of influential factors can be determined with the help of GIS and as per AHP and theory of variable weights. In addition, farmland fertility was e- valuated in an automatic and quantitative way and the spatial distribution pattern was analyzed as per fuzzy comprehensive evaluation. [Result] For farmlands at 58 505.027 8 hm2 in the city, farmlands from grade 1 to grade 8 account for 3.62%, 18.27%, 33.15%, 26.96%, 13.66%, 3.29%, 0.81% and 0.24%, respectively, which is in consistent with local condition. [Conclusion] These results have been applied di- rectly in test regions and constitute a rewarding exploration for fertility evaluation in South China.

  20. Prediction of Cultivated Land Change Based on Gray Series Forecasting Model: A Case of Puan County, Guizhou Province, China

    OpenAIRE

    He, Guangwei; Zhou, Dequan

    2009-01-01

    Both climate and cultivated land area of Puan County, Guizhou Province, China are briefly described. The six steps of Gray Series Forecasting Model are introduced, including generation of series, generation of mean value, establishment of GM (1,1) model, reducing reaction, reliability test, and extrapolation forecast. According to the data of cultivated land area in Puan County from the year 1998 to 2007, Gray System Theory is used to establish the Gray Series Forecasting Model of cultivated ...

  1. An Innovative Research on the Cultivation Pattern of Human Resources Management Professionals

    Directory of Open Access Journals (Sweden)

    Xia Cui

    2009-02-01

    Full Text Available With the approaching era of knowlege-based economy as well as the gradual universalization of China’s higher education, the quantity issue in talent cultivation has given way to the quality one consequently. Setting out from some problems in the cultivation of human resources management professional in China, this article puts forward patterns for objectives, curriculum systems as well as teaching methods in innovative talent cultivation.

  2. An Innovative Research on the Cultivation Pattern of Human Resources Management Professionals

    OpenAIRE

    Xia Cui; Yang Lu

    2009-01-01

    With the approaching era of knowlege-based economy as well as the gradual universalization of China’s higher education, the quantity issue in talent cultivation has given way to the quality one consequently. Setting out from some problems in the cultivation of human resources management professional in China, this article puts forward patterns for objectives, curriculum systems as well as teaching methods in innovative talent cultivation.

  3. Methods in Logic Based Control

    DEFF Research Database (Denmark)

    Christensen, Georg Kronborg

    1999-01-01

    Desing and theory of Logic Based Control systems.Boolean Algebra, Karnaugh Map, Quine McClusky's algorithm. Sequential control design. Logic Based Control Method, Cascade Control Method. Implementation techniques: relay, pneumatic, TTL/CMOS,PAL and PLC- and Soft_PLC implementation. PLC...

  4. Comparative Analysis and Cultivation of New Agricultural Business Entities Based on Comparison of Family Farms and Professional Cooperatives

    Institute of Scientific and Technical Information of China (English)

    Fang; YANG; Xingxi; LIU; Yingliang; ZHANG

    2014-01-01

    Cultivating new bussiness entities is the key to speeding up improving new agricultural production and operating system.This study discussed two representative entities under the agricultural production and operating system based on household contract management,namely,family farms and cooperatives.It introduced current development status of these two entities in Chongqing region,and discussed prominent problems of understanding,management,fund,personnel,technology,information,and system in the development process.In line with common problems such as single production structure and short supply of agricultural insurance,and different problems like organizational degree,it made comparative analysis.Finally,it is concluded that different institutional arrangement and pertinent support policy according to different development characteristics.

  5. Cultivating New-type Farmers Based on the Theory of Human Resources Development

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Under the direction of theory of human resources development,this thesis analyzes the impact of rural human resources development oncultivating new-type farmers.Firstly,it increases the input of rural basic education;secondly,it reinforces the vocational education and technologytraining;thirdly,it promotes the rural medical and public health services;fourthly,it quickens the rural labor transfer.The status quo of China’s ruralhuman resources has been analyzed as follows:in terms of the quantity of rural human resources,the status quo is large and quick-developed baseof rural human resources,high labor participatory rate,and constitution of low age;in terms of the quality of rural human resources,the status quois the ubiquitous low quality of rural human resources,low technological level of rural human resources,and overall low physical quality of farmers;in terms of the structure of rural human resources,the status quo is the irrational industrial structure distribution and imbalanced regional structuredistribution.The thesis also discusses the edification of theory of human resources development in cultivating new-type farmers.First,in terms ofthe control over quantity of rural human resources,it is to keep the stability of family planning policy,and expedite the transfer of rural surplus la-bor;second,in terms of promoting the quality of rural human resources,it is to bolster the development of reserve rural labor force resources,toconstruct the adult educational training system with Chinese characteristics,and to build rural primary health care system;third,in terms of adjus-ting the structure of rural human resources,it is to perfect rural human resources market,and adjust rural economical structure and talents struc-ture.

  6. Entropy-based benchmarking methods

    OpenAIRE

    Temurshoev, Umed

    2012-01-01

    We argue that benchmarking sign-volatile series should be based on the principle of movement and sign preservation, which states that a bench-marked series should reproduce the movement and signs in the original series. We show that the widely used variants of Denton (1971) method and the growth preservation method of Causey and Trager (1981) may violate this principle, while its requirements are explicitly taken into account in the pro-posed entropy-based benchmarking methods. Our illustrati...

  7. Activity based costing (ABC Method

    Directory of Open Access Journals (Sweden)

    Prof. Ph.D. Saveta Tudorache

    2008-05-01

    Full Text Available In the present paper the need and advantages are presented of using the Activity BasedCosting method, need arising from the need of solving the information pertinence issue. This issue has occurreddue to the limitation of classic methods in this field, limitation also reflected by the disadvantages ofsuch classic methods in establishing complete costs.

  8. Effect of cultivation method on the number and quality of young Santolina chamaecyparissus L. plants after wintering

    Directory of Open Access Journals (Sweden)

    Zdzisław Guzikowski

    2013-12-01

    Full Text Available The growth of young Santolina chamaecyparissus plants in a non-heated foil tunnel was rated depending on term of rooting cuttings and type of growing container in three cycles of study: 2005/06, 2006/07, 2007/08. Young plants obtained from cuttings rooted on 16th of August and 16th of September were planted on 16th of November in various containers and grown till 8th of May of the following year. Multipot pallets, cellulose pots and plastic pots were used for cultivation. In May, after 8-9 months the number of plants and their quality were defined after wintering. Type of containers and age of young plants did not have a significant effect on the wintering. The best quality of plants – high and with a large number of shoots – was obtained from cultivation in plastic pots.

  9. Replication methods and tools in high-throughput cultivation processes - recognizing potential variations of growth and product formation by on-line monitoring

    Directory of Open Access Journals (Sweden)

    Luft Karina

    2010-03-01

    Full Text Available Abstract Background High-throughput cultivations in microtiter plates are the method of choice to express proteins from recombinant clone libraries. Such processes typically include several steps, whereby some of them are linked by replication steps: transformation, plating, colony picking, preculture, main culture and induction. In this study, the effects of conventional replication methods and replication tools (8-channel pipette, 96-pin replicators: steel replicator with fixed or spring-loaded pins, plastic replicator with fixed pins on growth kinetics of Escherichia coli SCS1 pQE-30 pSE111 were observed. Growth was monitored with the BioLector, an on-line monitoring technique for microtiter plates. Furthermore, the influence of these effects on product formation of Escherichia coli pRhotHi-2-EcFbFP was investigated. Finally, a high-throughput cultivation process was simulated with Corynebacterium glutamicum pEKEx2-phoD-GFP, beginning at the colony picking step. Results Applying different replication tools and methods for one single strain resulted in high time differences of growth of the slowest and fastest growing culture. The shortest time difference (0.3 h was evaluated for the 96 cultures that were transferred with an 8-channel pipette from a thawed and mixed cryoculture and the longest time difference (6.9 h for cultures that were transferred with a steel replicator with fixed pins from a frozen cryoculture. The on-line monitoring of a simulated high-throughput cultivation process revealed strong variances in growth kinetics and a twofold difference in product formation. Another experiment showed that varying growth kinetics, caused by varying initial biomass concentrations (OD600 of 0.0125 to 0.2 led to strongly varying product formation upon induction at a defined point of time. Conclusions To improve the reproducibility of high-throughput cultivation processes and the comparability between different applied cultures, it is strongly

  10. A Multiple-Sessions Interactive Computer-Based Learning Tool for Ability Cultivation in Circuit Simulation

    Science.gov (United States)

    Xu, Q.; Lai, L. L.; Tse, N. C. F.; Ichiyanagi, K.

    2011-01-01

    An interactive computer-based learning tool with multiple sessions is proposed in this paper, which teaches students to think and helps them recognize the merits and limitations of simulation tools so as to improve their practical abilities in electrical circuit simulation based on the case of a power converter with progressive problems. The…

  11. Design and Implementation of Emergy-Based Sustainability Decision Assessment System for Protected Grape Cultivation

    OpenAIRE

    Jianying Feng; Jing Wang; Xiaoshuan Zhang; Fengtao Zhao; Radoslava Kanianska; Dong Tian

    2015-01-01

    The characteristic “high input and output” in protected production has caused some environmental and ecological issues. Hence, emergy-based sustainability assessments are necessary and valuable. However, traditional emergy analysis is time consuming, tedious, and inefficient. Such disadvantages can be addressed by the integration of emergy analysis with information technology. This paper reports the development of the emergy-based sustainability decision assessment system (ESDAS) for protecte...

  12. A Spiral Step-by-Step Educational Method for Cultivating Competent Embedded System Engineers to Meet Industry Demands

    Science.gov (United States)

    Jing,Lei; Cheng, Zixue; Wang, Junbo; Zhou, Yinghui

    2011-01-01

    Embedded system technologies are undergoing dramatic change. Competent embedded system engineers are becoming a scarce resource in the industry. Given this, universities should revise their specialist education to meet industry demands. In this paper, a spirally tight-coupled step-by-step educational method, based on an analysis of industry…

  13. A SSR-based composite genetic linkage map for the cultivated peanut (Arachis hypogaea L.) genome

    OpenAIRE

    Li Shaoxiong; Zhou Guiyuan; Liu Haiyan; Liang Xuanqiang; Chen Xiaoping; Hong Yanbin; Wen Shijie; Holbrook C Corley; Guo Baozhu

    2010-01-01

    Abstract Background The construction of genetic linkage maps for cultivated peanut (Arachis hypogaea L.) has and continues to be an important research goal to facilitate quantitative trait locus (QTL) analysis and gene tagging for use in a marker-assisted selection in breeding. Even though a few maps have been developed, they were constructed using diploid or interspecific tetraploid populations. The most recently published intra-specific map was constructed from the cross of cultivated peanu...

  14. Fruit protected cultivation in China

    Institute of Scientific and Technical Information of China (English)

    Gao Huajun; Wang Saoming; Wang Jiaxi

    2003-01-01

    Protected fruit cultivation in China has developed very quickly from the early 1990s, and now it is animportant branch in fruit cultivation. A brief review including fruit species, developing history, growing area, output, anddistribution in the whole country is made in the paper. Characteristics of the dominant kinds of greenhouse,environmental control methods, and standards of temperature, humidity, light and CO2 for different fruit species arepresented. Information on varieties, growing benefits, special management practices and other aspects of the main fruitspecies used for protected cultivation are also presented.

  15. Regional Cluster Development Measures and New Technology Based Firms Cultivation in Japan

    OpenAIRE

    Kirihata, Tetsuya

    2008-01-01

    The aim of this paper is to reveal significant factors within a cluster that influence the fostering of new technology based firms (hereafter abbreviated NTBFs) and determine which measures should be given priority in aiding regional improvement. In this paper, a questionnaire survey was conducted with project directors of regional knowledge cluster headquarters promoted by the Ministry of Education, Culture, Sports, Science and Technology, NTBFs, as well as, external specialists such as atto...

  16. Cultivable endophytic bacteria from leaf bases of Agave tequilana and their role as plant growth promoters

    OpenAIRE

    Julia del C. Martínez-Rodríguez; Marcela De la Mora-Amutio; Plascencia-Correa, Luis A.; Esmeralda Audelo-Regalado; Francisco R. Guardado; Elías Hernández-Sánchez; Peña-Ramírez, Yuri J.; Adelfo Escalante; Miguel J. Beltrán-García; Tetsuya Ogura

    2015-01-01

    Agave tequilana Weber var. 'Azul' is grown for the production of tequila, inulin and syrup. Diverse bacteria inhabit plant tissues and play a crucial role for plant health and growth. In this study culturable endophytic bacteria were extracted from leaf bases of 100 healthy Agave tequilana plants. In plant tissue bacteria occurred at mean population densities of 3 million CFU/g of fresh plant tissue. Three hundred endophytic strains were isolated and 16s rDNA sequences grouped the bacteria in...

  17. Recommendations for the regionalizing of coffee cultivation in Colombia: a methodological proposal based on agro-climatic indices.

    Directory of Open Access Journals (Sweden)

    Juan Carlos García L

    Full Text Available The Colombian National Federation of Coffee Growers (FNC conducted an agro-ecological zoning study based on climate, soil, and terrain of the Colombian coffee-growing regions (CCGR located in the tropics, between 1° and 11.5° N, in areas of complex topography. To support this study, a climate baseline was constructed at a spatial resolution of 5 km. Twenty-one bioclimatic indicators were drawn from this baseline data and from yield data for different coffee genotypes evaluated under conditions at eight experimental stations (ESs belonging to the National Center for Coffee Research (CENICAFÉ. Three topographic indicators were obtained from a digital elevation model (DEM. Zoning at a national level resulted in the differentiation of 12 agro-climatic zones. Altitude notably influenced zone differentiation, however other factors such as large air currents, low-pressure atmospheric systems, valleys of the great rivers, and physiography also played an important role. The strategy of zoning according to coffee-growing conditions will enable areas with the greatest potential for the development of coffee cultivation to be identified, criteria for future research to be generated, and the level of technology implementation to be assessed.

  18. Characterizing diversity based on nutritional and bioactive compositions of yam germplasm (Dioscorea spp. commonly cultivated in China

    Directory of Open Access Journals (Sweden)

    Zhi-Gang Wu

    2016-04-01

    Full Text Available Yams (Dioscorea spp. are widely cultivated as edible resources and medical materials in China. Characterizing chemical compositions in yam germplasm is crucial to determine their diversity and suitability for food and medicine applications. In this study, a core germplasm containing 25 yam landraces was used to create an effective classification of usage by characterizing their nutritive and medicinal compositions. All studied landraces exhibited high contents of starch from 60.7% to 80.6% dry weight (DW, protein (6.3–12.2% DW, minerals (especially Mg 326.8–544.7 mg/kg DW, and essential amino acids. Allantoin and dioscin varied considerably, with values of 0.62–1.49% DW and 0.032–0.092% DW, respectively. The quality variability of 25 yam landraces was clearly separated in light of UPGMA clustering and principal component analysis (PCA. Using an eigenvalue ≥1 as the cutoff, the first three principal components accounted for most of the total variability (62.33%. Classification was achieved based on the results of the measured parameters and principal component analysis scores. The results are of great help in determining appropriate application strategies for yam germplasm in China.

  19. Recommendations for the regionalizing of coffee cultivation in Colombia: a methodological proposal based on agro-climatic indices.

    Science.gov (United States)

    García L, Juan Carlos; Posada-Suárez, Húver; Läderach, Peter

    2014-01-01

    The Colombian National Federation of Coffee Growers (FNC) conducted an agro-ecological zoning study based on climate, soil, and terrain of the Colombian coffee-growing regions (CCGR) located in the tropics, between 1° and 11.5° N, in areas of complex topography. To support this study, a climate baseline was constructed at a spatial resolution of 5 km. Twenty-one bioclimatic indicators were drawn from this baseline data and from yield data for different coffee genotypes evaluated under conditions at eight experimental stations (ESs) belonging to the National Center for Coffee Research (CENICAFÉ). Three topographic indicators were obtained from a digital elevation model (DEM). Zoning at a national level resulted in the differentiation of 12 agro-climatic zones. Altitude notably influenced zone differentiation, however other factors such as large air currents, low-pressure atmospheric systems, valleys of the great rivers, and physiography also played an important role. The strategy of zoning according to coffee-growing conditions will enable areas with the greatest potential for the development of coffee cultivation to be identified, criteria for future research to be generated, and the level of technology implementation to be assessed. PMID:25436456

  20. Effect of PCL/PEG-Based Membranes on Actinorhodin Production in Streptomyces coelicolor Cultivations.

    Science.gov (United States)

    Scaffaro, Roberto; Lopresti, Francesco; Sutera, Alberto; Botta, Luigi; Fontana, Rosa Maria; Puglia, Anna Maria; Gallo, Giuseppe

    2016-05-01

    The actinomycetes, Gram-positive filamentous bacteria, are the most prolific source of natural occurring antibiotics. At an industrial level, antibiotics from actinomycete strains are produced by means of submerged fermentations, where one of the major factors negatively affecting bioproductivity is the pellet-shaped biomass growth. The immobilization of microorganisms on properly chosen supports prevents cell-cell aggregation resulting in improving the biosynthetic capability. Thus, novel porous biopolymer-based devices are developed by combining melt mixing and particulate leaching. In particular, polycaprolactone (PCL), polyethylene glycol (PEG), and sodium chloride (NaCl) with different grain sizes are used to prepare PCL/PEG/NaCl blends in the melt. These blends are then leached to obtain PCL-based porous membranes that are used as solid support for the growth of Streptomyces coelicolor, a model streptomycete used to produce various antibiotics including the blue colored actinorhodin (ACT). Thereafter, the effect of the devices' characteristics on the bacterial growth and on the production ACT is evaluated. The results showed that ACT production is strongly dependent on the pore size distribution of the device. Moreover, membranes with pores ranging from 90 to 110 μm are able to offer a potential improvement in volumetric productivity of ACT if compared to conventional submerged liquid culture. PMID:26762618

  1. Robot-based Learning : Toward Cultivation of Information Technology Skills for Mechanical Engineering Students

    Science.gov (United States)

    Hanajima, Naohiko; Yamashita, Mitsuhisa; Kazama, Toshiharu; Yuasa, Tomonori; Niida, Yoichi; Aizu, Yoshihisa; Hikita, Hiromitsu

    Today there is increasing development of products in which embedded microprocessors are installed in a wide variety of industrial fields including mechanical industries. Mechanical engineers will be asked to develop such embedded systems in the future. To educate mechanical engineering students who possess information technology skills, for five years we have offered practical classes in which the students build a mobile robot with an embedded microprocessor and compete in a robot-triathlon race. The students have an incentive to program their robot to finish the race. We call this style of learning “robot-based learning.” In this paper, we discuss the efficiency of and problems in our practical classes as derived from information gained in surveys. In addition, we verify how the engineering design abilities of the students are improved through their participation in these classes.

  2. CULTIVATION OF CLADOCERAN (CLADOCERA FOR INCREASING PROVISION OF YOUNG-OF-THE-YEAR CARP (CYPRINUS CARPIO WITH NATURAL FEEDS (REVIEW

    Directory of Open Access Journals (Sweden)

    A. Tuchapska

    2014-06-01

    Full Text Available Purpose. Natural feeds are important in pond fish diet because they contain all nutrients necessary for fish growth and development. The share of natural feeds in fish diet has great effect on fish growth and immunity, assimilation of artificial feeds. The main way of assured procurement of natural feeds for fish feeding at different stages of their development is artificial cultivation of aquatic organisms. However, cultivation of natural feeds is not virtually performed in aquaculture enterprises of Ukraine, therefore an analysis of available data on zooplankton cultivation is important for looking for optimal and economically profitable methods of enrichment of pond fish diet with natural feeds. Methodology. Methods of cladoceran cultivation were the object of the study, material for the study – literature data on ways and methods of zooplankton cultivation. Findings. Cultivation of various species of zooplankton is performed for feeding of pond fish on different life stages. Main object of cultivation in aquaculture is Daphnia magna Straus, juvenile forms of which are consumed by fish larvae, while adult organisms are the most valuable for yearlings and older fish. The efficiency of hydrobiont cultivation highly depends on the selected object, containers, where cladocerans are cultivated, optimum conditions, peculiarities of water supply, species, and application of fertilizers and feeds. Originality. The highest production of zooplankton can be obtained when cultivating D. magna in tanks with continuous flow and in net cages installed in ponds under condition of ensuring requirements of the culture in bacterial and algae feeds (due to application of fertilizers and feeding with feeds and microalgae taking into account their presence in water, which is in the tank-cultivator. Practical value. Simplicity of the methods and high efficiency of zooplankton cultivation for preparation of pond fish juveniles is the basis of its wide use in

  3. Ecological niche separation in the Polynucleobacter subclusters linked to quality of dissolved organic matter: a demonstration using a high sensitivity cultivation-based approach.

    Science.gov (United States)

    Watanabe, Keiji; Komatsu, Nobuyuki; Kitamura, Tatsumi; Ishii, Yuichi; Park, Ho-Dong; Miyata, Ryo; Noda, Naohiro; Sekiguchi, Yuji; Satou, Takayuki; Watanabe, Mirai; Yamamura, Shigeki; Imai, Akio; Hayashi, Seiji

    2012-09-01

    The free-living, cosmopolitan, freshwater betaproteobacterial bacterioplankton genus Polynucleobacter was detected in different years in 11 lakes of varying types and a river using the size-exclusion assay method (SEAM). Of the 350 strains isolated, 228 (65.1%) were affiliated with the Polynucleobacter subclusters PnecC (30.0%) and PnecD (35.1%). Significant positive correlations between fluorescence in situ hybridization and SEAM data were observed in the relative abundance of PnecC and PnecD bacteria to Polynucleobacter communities (PnecC + PnecD). Isolates were mainly PnecC bacteria in the samples with a high specific UV absorbance at 254 nm (SUVA(254) ), and a low total hydrolysable neutral carbohydrate and amino acid (THneutralCH + THAA) content of the dissolved organic matter (DOM) fraction, which is known to be correlated with a high humic content. In contrast, the PnecD bacteria were abundant in samples with high chlorophyll a and/or THneutralCH + THAA concentrations, indicative of primary productivity. With few exceptions, differences in the relative abundance of PnecC and PnecD in each sample, determined using a high-sensitivity cultivation-based approach, were due to DOM quality. These results suggest that the major DOM component in the field, which is allochthonously or autochthonously derived, is a key factor for ecological niche separation between PnecC and PnecD subclusters. PMID:22759205

  4. Cultivating Engineering Ethics and Critical Thinking: A Systematic and Cross-Cultural Education Approach Using Problem-Based Learning

    Science.gov (United States)

    Chang, Pei-Fen; Wang, Dau-Chung

    2011-01-01

    In May 2008, the worst earthquake in more than three decades struck southwest China, killing more than 80,000 people. The complexity of this earthquake makes it an ideal case study to clarify the intertwined issues of ethics in engineering and to help cultivate critical thinking skills. This paper first explores the need to encourage engineering…

  5. Cellulase and xylanase productions by isolated Amazon Bacillus strains using soybean industrial residue based solid-state cultivation

    Directory of Open Access Journals (Sweden)

    Heck Júlio X.

    2002-01-01

    Full Text Available In Brazil, a large amount of a fibrous residue is generated as result of soybean (Glycine max protein production. This material, which is rich in hemicellulose and cellulose, can be used in solid state cultivations for the production of valuable metabolites and enzymes. In this work, we studied the bioconversion of this residue by bacteria strains isolated from water and soil collected in the Amazon region. Five strains among 87 isolated bacteria selected for their ability to produce either celullases or xylanases were cultivated on the aforementioned residue. From strain BL62, identified as Bacillus subtilis, it was obtained a preparation showing the highest specific cellulase activity, 1.08 UI/mg protein within 24 hours of growth. Concerning xylanase, the isolate BL53, also identified as Bacillus subtilis, showed the highest specific activity for this enzyme, 5.19 UI/mg protein within 72 hours of cultivation. It has also been observed the production of proteases that were associated with the loss of cellulase and xylanase activities. These results indicated that the selected microorganisms, and the cultivation process, have great biotechnological potential.

  6. Tree-based ranking methods

    OpenAIRE

    Clémençon, Stéphan; Vayatis, Nicolas

    2008-01-01

    The paper investigates how recursive partitioning methods can be adapted to the bipartite ranking problem. In ranking, the pursued goal is global: based on past data, define an order on the whole input space X, so that positive instances take up the top ranks with maximum probability. The most natural way to order all instances consists of projecting the input data onto the real line through a real-valued scoring function s and use the natural order on R. The accuracy of the ordering induced ...

  7. Exploring the complex response to linuron of bacterial communities from biopurification systems by means of cultivation-independent methods.

    Science.gov (United States)

    Dealtry, Simone; Nour, Eman H; Holmsgaard, Peter N; Ding, Guo-Chun; Weichelt, Viola; Dunon, Vincent; Heuer, Holger; Hansen, Lars H; Sørensen, Søren J; Springael, Dirk; Smalla, Kornelia

    2016-02-01

    On-farm biopurification systems (BPSs) treat pesticide-contaminated wastewater at farms through biodegradation and sorption processes. However, information on the microbiota involved in pesticide removal in BPSs is scarce. Here we report on the response of BPS bacterial communities to the herbicide linuron (BPS(+)) compared with the control (BPS(-)) in a microcosm experiment. Both denaturing gradient gel electrophoresis (DGGE) and pyrosequencing of 16S rRNA gene fragments amplified from community DNA indicated shifts in the bacterial community after linuron application. Responding populations belonged to taxa that were previously reported from linuron degrading consortia cultivated from soil (Hyphomicrobiaceae, Comamonadaceae, Micrococcaceae). In addition, numerous taxa with increased relative abundance were identified that were previously not associated with linuron degradation. The relative abundance of IncP-1 korB copies increased in response to linuron application. Amplicon pyrosequencing of IncP-1 trfA genes revealed a high IncP-1 plasmid diversity and suggested that populations carrying IncP-1β plasmids increased in relative abundance. Transferable mercury resistance plasmids were exogenously captured from BPS(+)/BPS(-), and in three transconjugants from BPS(+) the gene hylA was detected. Our data suggest the existence of a multispecies linuron degrading bacterial food web and an involvement of IncP-1 plasmids in the adaptation of bacterial communities to pesticide pollution in BPSs. PMID:26705572

  8. Research on the Cultivation Mechanism of Innovative Talents Based on TPB%基于 TPB的创新型人才培养机制研究

    Institute of Scientific and Technical Information of China (English)

    孔德议

    2015-01-01

    The cultivation of innovative talents has a profound significance for China in transition. The paper analyzes innovative talent training mechanism and universities based on the theory of planned behavior (TPB) to explore how to cultivate talents to satisfy the needs of the society un‐der the existing circumstances in China. The paper analyzes the influence factors of creative talents training from the subjective norm ,behavioral attitude and perceived behavior control three aspects respectively. And then it constructs the innovative talent cultivation mechanism based on TPB model and puts forward the improvement suggestions and countermeasures of cultivating innovative talents in colleges and universities .%创新型人才的培养对处于转型中的中国有着深远的意义。基于计划行为理论(简称T PB )对创新型人才培养机制进行分析研究,旨在探索在中国现有的大环境下如何培养出适应社会需要的人才。从主观规范,行为态度和感知行为控制三方面分别对应分析创新型人才培养的影响因素,在此基础上构建了基于 T PB的创新型人才培养机制模型并提出改进培养创新型人才的相关建议和对策。

  9. Cultivating nutrition

    OpenAIRE

    Levin, Carol E.; Long, Jennifer; Simler, Kenneth R.; Johnson-Welch, Charlotte

    2003-01-01

    "Over the past decade, donor-funded policies and programs designed to address undernutrition in the Global South have shifted away from agriculture-based strategies toward nutrient supplementation and food fortification programs. Given the potential benefits resulting from agriculture-based nutrition interventions, this study uses Q methodology to explore the views of a range of stakeholders from both developed and developing countries on the value of—and constraints related to—gender-sensiti...

  10. Bioreactors for high cell density and continuous multi-stage cultivations: options for process intensification in cell culture-based viral vaccine production.

    Science.gov (United States)

    Tapia, Felipe; Vázquez-Ramírez, Daniel; Genzel, Yvonne; Reichl, Udo

    2016-03-01

    With an increasing demand for efficacious, safe, and affordable vaccines for human and animal use, process intensification in cell culture-based viral vaccine production demands advanced process strategies to overcome the limitations of conventional batch cultivations. However, the use of fed-batch, perfusion, or continuous modes to drive processes at high cell density (HCD) and overextended operating times has so far been little explored in large-scale viral vaccine manufacturing. Also, possible reductions in cell-specific virus yields for HCD cultivations have been reported frequently. Taking into account that vaccine production is one of the most heavily regulated industries in the pharmaceutical sector with tough margins to meet, it is understandable that process intensification is being considered by both academia and industry as a next step toward more efficient viral vaccine production processes only recently. Compared to conventional batch processes, fed-batch and perfusion strategies could result in ten to a hundred times higher product yields. Both cultivation strategies can be implemented to achieve cell concentrations exceeding 10(7) cells/mL or even 10(8) cells/mL, while keeping low levels of metabolites that potentially inhibit cell growth and virus replication. The trend towards HCD processes is supported by development of GMP-compliant cultivation platforms, i.e., acoustic settlers, hollow fiber bioreactors, and hollow fiber-based perfusion systems including tangential flow filtration (TFF) or alternating tangential flow (ATF) technologies. In this review, these process modes are discussed in detail and compared with conventional batch processes based on productivity indicators such as space-time yield, cell concentration, and product titers. In addition, options for the production of viral vaccines in continuous multi-stage bioreactors such as two- and three-stage systems are addressed. While such systems have shown similar virus titers compared to

  11. DISPLACEMENT BASED SEISMIC DESIGN METHODS.

    Energy Technology Data Exchange (ETDEWEB)

    HOFMAYER,C.MILLER,C.WANG,Y.COSTELLO,J.

    2003-07-15

    A research effort was undertaken to determine the need for any changes to USNRC's seismic regulatory practice to reflect the move, in the earthquake engineering community, toward using expected displacement rather than force (or stress) as the basis for assessing design adequacy. The research explored the extent to which displacement based seismic design methods, such as given in FEMA 273, could be useful for reviewing nuclear power stations. Two structures common to nuclear power plants were chosen to compare the results of the analysis models used. The first structure is a four-story frame structure with shear walls providing the primary lateral load system, referred herein as the shear wall model. The second structure is the turbine building of the Diablo Canyon nuclear power plant. The models were analyzed using both displacement based (pushover) analysis and nonlinear dynamic analysis. In addition, for the shear wall model an elastic analysis with ductility factors applied was also performed. The objectives of the work were to compare the results between the analyses, and to develop insights regarding the work that would be needed before the displacement based analysis methodology could be considered applicable to facilities licensed by the NRC. A summary of the research results, which were published in NUREGICR-6719 in July 2001, is presented in this paper.

  12. Multi-Suitability Evaluation of Cultivated Land in Houhu Farm Area Based on GIS and Niche-Fitness

    Institute of Scientific and Technical Information of China (English)

    LIU Li-wei; NIE Yan; ZHOU Yong

    2005-01-01

    The niche-fitness and limited factors of rice, wheat, cotton and rape in Houhu Farm area of Jianghan Plain are analyzed by weighted average model and limited factor model. The niche-fitness theory and GIS are applied to the research on multi-suitability evaluation. The high values of the niche-fitness (the maximum is 0.958) indicate that the environmental conditions basically meet the requirements of crops in the area. Suitability evaluation of single crop shows that the degrees of suitability are satisfactory. Comprehensive suitability evaluation shows that the acreage for grade 1 (highly suitable for cultivation) accounts for 18.16% of the total cultivated land area, whereas the acreage for grade 3 (critically suitable) and grade 4 (unsuitable) only for 8.9%. The main factors limiting the potential land productivity are cation exchange capacity (CEC), available phosphorus content and accessibility level of road.

  13. Research on Monitoring Area Division of Quality Grade Changes in County Cultivated Land and Technology of Deploying Monitoring Point

    Institute of Scientific and Technical Information of China (English)

    Wei; WEI; Lijun; LIAO; Jianxin; YU

    2013-01-01

    It is an important means in management of improving both the quality and quantity of cultivated land to monitor grade changes in cultivated land quality. How to deploy monitoring network system and its point reasonably and roundly are the key to the technology of monitoring grade changes in cultivated land quality by monitoring grade changes in cultivated land quality dynamically in order to obtain the information to the index of cultivated land quality and its changes based on the existing achievements of farmland classification and grading. Spatial analysis method is used to demarcate monitoring area and deploy monitoring point according to ARCGIS,of which the result can meet the demand for monitoring grade changes in cultivated land.

  14. 非培养法检测阴道微生态的进展%Research Progress of Detecting Vagina Microecology by Cultivation-Independent Methods

    Institute of Scientific and Technical Information of China (English)

    陈晴

    2012-01-01

    女性阴道内寄生着多种微生物,鉴定阴道中存在的微生物有助于了解其在健康和疾病时的作用,但是许多微生物难以通过培养将其分离鉴定.近年来,一系列分子生物学的方法,如基于16S rRNA基因聚合酶链反应扩增法等用于研究阴道微生态并取得了较大的进展.利用分子生物学的非培养法检测女性阴道微生物发现,不论在健康女性还是细菌性阴道病(BV)患者中惰性乳杆菌是普遍存在的乳杆菌;还发现许多新的厌氧菌与BV高度相关,如阴道阿托波菌、阴道加德纳菌、BVAB1等.%The human vagina hosts many microbes. Identifying all kinds of microbes in vagina will help to better understand their roles in heath and disease. However,many microbes are difficult to be identified by cultivation-dependent methods. In recent years, a series of molecular approaches, such as PCR amplification of 16S rRNA gene,are used to study the vagina microecology and have made considerable progress. The use of molecular, cultivation-independent methods have revealed :lactobacillus inert is the ubiquitous nature in women with or without bacterial vaginosis( BV ); some novel anaerobes are strongly associated with BV, such as Atopobium vaginae, Gardnerella vaginalis, BVAB1 and so on.

  15. Rapid, Simple, and Specific Cultivation-Based Method for Detection of Pectinatus spp. in Brewery Samples

    Czech Academy of Sciences Publication Activity Database

    Matoulková, D.; Kosař, K.; Sigler, Karel

    2012-01-01

    Roč. 70, č. 1 (2012), s. 29-34. ISSN 0361-0470 R&D Projects: GA MŠk 1M0570 Institutional research plan: CEZ:AV0Z50200510 Keywords : Beer spoilage * Hop compounds * MRS medium Subject RIV: EE - Microbiology, Virology Impact factor: 1.000, year: 2012

  16. DNA-based stable isotope probing coupled with cultivation methods implicates Methylophaga in hydrocarbon degradation

    OpenAIRE

    TonyGutierrez

    2014-01-01

    Marine hydrocarbon-degrading bacteria perform a fundamental role in the oxidation and ultimate removal of crude oil and its petrochemical derivatives in coastal and open ocean environments. Those with an almost exclusive ability to utilize hydrocarbons as a sole carbon and energy source have been found confined to just a few genera. Here we used stable isotope probing (SIP), a valuable tool to link the phylogeny and function of targeted microbial groups, to investigate hydrocarbon-degrading b...

  17. Evaluation on Cultivated Land Ecological Security of Leshan City Based on Improved Set Pair Analysis%基于改进 SPA 的乐山市耕地生态安全评价

    Institute of Scientific and Technical Information of China (English)

    刘小波; 秦天彬; 周宝同; 涂建军

    2016-01-01

    Evaluation of cultivated land ecological security is comprehensive diagnosis for complex system of farmland ecosystem ,and it is an important content to measure regional sustainable development .T his pa‐per takes Leshan City of Sichuan province for example ,on the basis of defining the connotation of cultivat‐ed land ecological security ,constructing an evaluation index system base on the Pressure‐State‐Response (PSR)model .With the improved SPA the cultivated land ecological security in Leshan City and its districts in 2007 ,2009 ,and 2012 has been evaluated . T hen the change factors are discussed and conclusions are summarized as follow s :1) T he level of cultivated land ecological security in Leshan City is increasing on the w hole ,safe level is gradually improved from “critically safe”to“safe” ,the level of cultivated land eco‐logical security in each district is improved in varying degree .2) The quality and quantity of cultivated land resources ,the good points and bad points of ecological environment ,and the level of economy are the main factors which lead to the differences of cultivated land ecological security between the south and the north . 3) The improved SPA method can reveal the degree of differentiation of evaluation index system for culti‐vated land ecological security ,and extract the intermediate information of comprehensive evaluation re‐sults ,w hich has some practical value in the evaluation of cultivated land ecological security .%耕地生态安全评价是对农田生态复合系统的全面诊断,也是测度区域可持续发展的重要内容。本文以四川省乐山市为例,在界定耕地生态安全内涵的基础上,构建基于PSR模型的评价指标体系,并采用改进的SPA法分别对乐山市及各区县2007,2009,2012年耕地生态安全进行评价,并对其变化因素进行了分析。结果表明:1)乐山市耕地生态安全水平整体上呈上升趋势,

  18. Impacts of energy crop cultivation on nature and landscape. Development and application of an evaluation method; Auswirkungen des Energiepflanzenanbaus auf Natur und Landschaft. Entwicklung und Anwendung einer Bewertungsmethode

    Energy Technology Data Exchange (ETDEWEB)

    Wiehe, Julia

    2011-08-15

    For long-term planning, knowledge about the interrelationship of effects of the cultivation method and the sensitivity of ecological balance is essential. Hence, the objective of this thesis is the development of a method for the evaluation of the impacts of bioenergy crop production for biogas use on the natural environment. The developed method is in alignment with existing methods. It is also in alignment with those methods used within the practice of landscape planning, so that the method as well as the derived conclusions can be implemented into landscape planning practice in the future. The evaluation method has been applied in the three model regions Hildesheim, Soltau-Fallingbostel and Emsland. These test areas represent different physical regions in Lower Saxony and typical agricultural production conditions. On the basis of these results, general statements on the impact of bioenergy crop production on the ecological balance of the area can be made. [German] Grundlage fuer eine vorausschauende Steuerung des Ausbaus erneuerbarer Energien ist die Kenntnis der Zusammenhaenge der Wirkung des Energiepflanzenanbaus und der Empfindlichkeit des Naturhaushaltes. Ziel der Arbeit ist daher die Erarbeitung einer Methode zur umfassenden Bewertung dieser Auswirkungen auf den Naturhaushalt. Die Methode orientiert sich an bereits bestehenden und in der Praxis der Landschaftsplanung angewendeten Bewertungsmethoden, so dass sie ebenso wie die daraus abgeleiteten Schlussfolgerungen zukuenftig Eingang in die Planungspraxis finden kann. Die Bewertungsmethode wird in den drei Modellregionen Hildesheim, Soltau-Fallingbostel und Emsland angewendet, mit denen die verschiedenen Naturraeume und fuer Niedersachsen typischen landwirtschaftlichen Produktionsbedingungen abgebildet werden. Auf Grundlage dieser Ergebnisse koennen dann allgemeine Aussagen zu den Auswirkungen des Energiepflanzenanbaus auf den Naturhaushalt gemacht werden.

  19. PBL教学法在实验教学中对学生批判性思维能力的培养%Application of PBL teaching method in cultivation of critical thinking ability of students in experimental teaching

    Institute of Scientific and Technical Information of China (English)

    秦海宏; 葛伟; 孙雪洁

    2013-01-01

    The PBL teaching method is a teaching mode based on problems.It has significant effect on the formation of crilical thinking ability of the nursery students in experimental teaching.In our study,the PBL was adopted in the experimental group,while traditional teaching method was used in the control group.CCTDI-CV was applied to assess the critical thinking ability of the students in these above two groups.It was found that the critical thinking ability of the students in the experimental group is significantly higher than that in the control group.This suggested that the PBL is beneficial to cultivate the critical thinking ability of the students.This paper provides an evidence for improving the teaching mode and way of the cultivation of critical thinking ability in nursery practice teaching.%PBL教学是以问题为基础的模式,在实验教学中对培养学生批判性思维能力的效果显著.在对实验组采用PBL教学法教学、对照组采用传统的实验教学方法教学的基础上,应用CCTDI-CV评价2种教学方法对学生批判性思维能力的影响.经分析发现,PBL教学组学生的批判性思维能力高于对照组,两组间差异显著.实验教学中的PBL教学模式有助于学生批判性思维能力的培养.

  20. Comparison of soil thermal properties in cultivated fields determined using soil water content measured by two methods

    OpenAIRE

    Kossowski J.; Usowicz B.

    1999-01-01

    Results of the measurements of water content in the topsoil layer (1-6 cm) in fields with various crops obtained by gravimetric and reflectometric (TDR) methods have been used for the calculations of soil volumetric heat capacity, thermal conductivity, and diffusivity. Calculation values of individual soil thermal properties obtained in the two ways were then analysed by means of statistical and geostatistical methods and compared (correlation coefficients, regression equations, difference di...

  1. In vitro cultivation of rat bone marrow mesenchymal stem cells and establishment of pEGFP/Ang-1 transfection method

    Institute of Scientific and Technical Information of China (English)

    Xiu-Qun; Zhang; Long; Wang; Shu-Li; Zhao; Wei; Xu

    2014-01-01

    Objective:To obtain the bone marrow mesenchymal stem cells(BMSCs).complete phenotypic identification and successfully transfecl rat BMSCs by recombinant plasmid pF.GFP/Ang-1.Methods:BMSCs were isolated from bone marrow using density gradient centrifugation method and adherence screening method,and purified.Then the recombinant plasmid pEGFP/Ang-1was used to transfect BMSCs and the positive clones were obtained by the screen of C418 and observed under light microscopy inversely.Green fluorescent exhibited by protein was enhanced to measure the change time of the expression amount of Ang-1.Results:BMSCs cell lines were obtained successfully by adherence screening method and density gradient ccntrifugation.Ang-1 recombinant plasmid was transfected smoothly into rat BMSCs,which can express Ang-1 for 3 d and decreased after 7 d.Conclusions:Adherence screening method und density gradient ceiilrifugation can be effective methods lo obtain BMSCs with high purity and rapid proliferation.Besides,the expression of transfected recombinant plasmid pEGFP/Ang-1 in rat BMSCs is satisfactory.

  2. 县域耕地后备资源与规划期内数量质量并重的占补平衡分析%Analysis on county based reserved resource for cultivated land and quality-quantity requisition-compensation balance in planning period

    Institute of Scientific and Technical Information of China (English)

    高星; 吴克宁; 郧文聚; 唐程杰; 许实; 麻战洪

    2015-01-01

    Reserved resource for cultivated land is an important supplement to cultivated land, and it plays an important role in requisition-compensation balance, however, it has disadvantages of subjectiveness and emphasizing on quantity in cultivated land supplement. It is necessary to play the role of farmland reserve resources to replenish cultivated land for the realization of the goal of farmland protection in the overall planning period of county land use. Data used for study are from the results of the new round of ‘survey and evaluation of reserved resource for cultivated land’. A balancing method, which focused on both quantity and quality and was based on the ratio of added cultivated land and grade conversion coefficient, were proposed to analyze the capability of requisition-compensation balance in the planning period. And we analyzed the capacity to achieve the balance of arable land in the county planning period. The coefficient of newly increased cultivated land was developed by the weighted average of the newly cultivated land rate. The conversion coefficient of requisition and compensation of arable land is based on the national unified research results of arable land’s requisition compensation balance according to grade conversion coefficient from Land Resources and Land Rehabilitation Center. Taking Yuanjiang City, Hunan Province as an example, we calculated the area of reserved resource for cultivated land needed for realizing both quantitative and qualitative balance. According to the "Yuanjiang City land use overall planning (during 2006-2020)", the new construction area of farmland was expected to not more than 899.2 hm2 until 2020, the quality of new construction occupation of cultivated land focused on the level of 1-5, whose composition ratios were 40.61%, 16.15%, 22.73%, 18.8% and 1.72%, respectively. The reserved resource area of cultivated land in Yuanjiang City was 1 744.14 hm2, of which 1 447.64 hm2 cultivated land can be added after the

  3. GIS-based assessment of land suitability for alfalfa cultivation: a case study in the dry continental steppes of northern China

    Directory of Open Access Journals (Sweden)

    Fei Deng

    2014-03-01

    Full Text Available Alfalfa (Medicago sativa L. is the most valuable perennial forage grass in northern China. We selected 12 ecological criteria and 4 socioeconomic criteria to calculate the suitability of land for alfalfa cultivation in the Xilingol League of the Inner Mongolia Autonomous Region. We combined ecological suitability assessment with fuzzy analysis to standardize the criteria. We used the analytical hierarchy process to determine the weight of these criteria, and used multi-criterion decision analysis (MCDA to aggregate the criteria. We then calculated the suitability score for each evaluation unit. The suitability was divided into highly, moderately, and marginally suitable, or unsuitable, using the geoprocessing module of ArcGIS 9.3 according to the FAO land suitability classification. We found that highly suitable areas covered 10,799.84 km2, accounting for 5.3% of the total area, and unsuitable areas covered 99,235.87 km2, accounting for 48.9% of the total area. Combining the fuzzy analysis method with the theory of ecological suitability and the MCDA method to evaluate the suitability of land for alfalfa cultivation provided insights that can guide decisionmakers and farmers to make more practical and scientific decisions.

  4. Development of an irrigation control device based on solar radiation and its adaptability for cultivation of high soluble solid tomato fruit in root zone restriction culture

    International Nuclear Information System (INIS)

    An irrigation control device based on solar radiation was developed to allow automatic irrigation management for high soluble solid tomato fruit production in root zone restriction culture. Its adaptability for long-term cultivation (planting carried out in early September and harvesting ending in late June) of high soluble solid tomato fruit in root zone restriction culture was examined. The following results were obtained: 1. The control device was composed of generally available electronic parts. A change of setting was possible for the irrigation starting point, the irrigation time period, and the once amount of irrigation. For the first irrigation of the day, one of two irrigation control modes can be chosen; the first determines irrigation dependent on the solar radiation after the irrigated time of the previous day. The second mode irrigates at a set time. 2. The correlation between the total integrated solar radiation and the evapotranspiration rate of tomato plants were investigated. Positive correlations were observed for each month from October to June. Moreover, total integrated solar radiation per unit evapotranspiration was different for each month. 3. In long-term cultivation of tomato fruit using this device, the marketable yield of high soluble solid tomato fruit (more than Brix 8%) was 9.7t/10a. 4. This device exhibited the necessary adaptability for use in long-term cultivation of high soluble solid tomato fruit in root zone restriction culture, by changing the set value of the irrigation starting point and the irrigation time period in accordance with the growth period

  5. High-density Linkage Map of Cultivated Allotetraploid Cotton Based on SSR, TRAP, SRAP and AFLP Markers

    Institute of Scientific and Technical Information of China (English)

    Jiwen Yu; Shuxun Yu; Cairui Lu; Wu Wang; Shuli Fan; Meizhen Song; Zhongxu Lin; Xianlong Zhang; Jinfa Zhang

    2007-01-01

    A high-density linkage map was constructed for an F2 population derived from an interspecific cross of cultivated allotetraploid species between Gossyplum hirsutum L. and G. barbadense L. A total of 186 F2 individuals from the interspecific cross of "CRI 36 × Hai 7124" were genotyped at 1 252 polymorphic loci including a novel marker system,target region amplification polymorphism (TRAP). The map consists of 1 097 markers, including 697 simple sequence repeats (SSRs), 171 TRAPs, 129 sequence-related amplified polymorphisms, 98 amplified fragment length polymorphisms, and two morphological markers, and spanned 4 536.7 cM with an average genetic distance of 4.1 cM per marker. Using 45 duplicated SSR loci among chromosomes, 11 of the 13 pairs of homologous chromosomes were identified in tetraplold cotton. This map will provide an essential resource for high resolution mapping of quantitative trait loci and molecular breeding in cotton.

  6. Objective, Way and Method of Faculty Management Based on Ergonomics

    Science.gov (United States)

    WANG, Hong-bin; Liu, Yu-hua

    2008-01-01

    The core problem that influences educational quality of talents in colleges and universities is the faculty management. Without advanced faculty, it is difficult to cultivate excellent talents. With regard to some problems in present faculty construction of colleges and universities, this paper puts forward the new objectives, ways and methods of…

  7. Method development for the determination of iron in milligram amounts of rice plants (Oryza sativa L.) from cultivation experiments using graphite furnace atomic absorption spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Marcia M.; Vale, Maria Goreti R.; Damin, Isabel C. Ferreira [Instituto de Quimica, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Goncalves 9500, 91501-970, Porto Alegre RS (Brazil); Welz, Bernhard [Departamento de Quimica, Universidade Federal de Santa Catarina (UFSC), 88040-900, Florianopolis SC (Brazil); Mandaji, Marcos; Fett, Janette P. [Departamento de Botanica e Centro de Biotecnologia, Instituto de Biociencias, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Goncalves 9500, 91501-970, Porto Alegre RS (Brazil)

    2003-09-01

    The amount of sample that is available for analysis in laboratory plant cultivation experiments is usually very limited. Highly sensitive analytical techniques are therefore required, even for elements that are present in the plants at mg g{sup -1} concentrations, and graphite furnace atomic absorption spectrometry (GFAAS) was chosen in this work because of its micro-sampling capability, and its relatively simple operation. Four micro-methods were investigated for the determination of iron in roots and leaves of rice plants: (i) a micro-digestion with nitric and hydrochloric acids, (ii) a slurry procedure using tetramethylammonium hydroxide (TMAH) tissue solubilizer, (iii) a slurry prepared in 1.4 mol L{sup -1} nitric acid, and treated in an ultrasonic bath, and (iv) the direct analysis of solid samples. The micro-digestion was suffering from high blank values and contamination problems, so that it could not be recommended for routine purposes. The TMAH method exhibited poor precision and occasional low recoveries, particularly for real samples. Direct solid sampling analysis gave results similar to those obtained with the slurry technique with ultrasonic agitation for the determination of iron in certified reference materials with iron content up to about 100 {mu}g g{sup -1}, but was too sensitive for the investigated rice plants, which had an iron content up to several mg g{sup -1}. The slurry technique with ultrasonic treatment of the samples, suspended in dilute nitric acid, was finally adopted as the method of choice. The method was then applied for the determination of iron in the leaves and in different compartments of the roots of two rice cultivars, one sensitive to iron toxicity, an important nutritional disorder, and the other one resistant to iron toxicity. The results suggest that the higher resistance to iron toxicity of the second cultivar is due to a smaller uptake of iron from the soil, resulting in lower iron levels in all compartments of the

  8. COMPANY VALUATION METHODS BASED ON PATRIMONY

    Directory of Open Access Journals (Sweden)

    SUCIU GHEORGHE

    2013-02-01

    Full Text Available The methods used for the company valuation can be divided into 3 main groups: methods based on patrimony,methods based on financial performance, methods based both on patrimony and on performance. The companyvaluation methods based on patrimony are implemented taking into account the balance sheet or the financialstatement. The financial statement refers to that type of balance in which the assets are arranged according to liquidity,and the liabilities according to their financial maturity date. The patrimonial methods are based on the principle thatthe value of the company equals that of the patrimony it owns. From a legal point of view, the patrimony refers to allthe rights and obligations of a company. The valuation of companies based on their financial performance can be donein 3 ways: the return value, the yield value, the present value of the cash flows. The mixed methods depend both onpatrimony and on financial performance or can make use of other methods.

  9. Based on Penalty Function Method

    Directory of Open Access Journals (Sweden)

    Ishaq Baba

    2015-01-01

    Full Text Available The dual response surface for simultaneously optimizing the mean and variance models as separate functions suffers some deficiencies in handling the tradeoffs between bias and variance components of mean squared error (MSE. In this paper, the accuracy of the predicted response is given a serious attention in the determination of the optimum setting conditions. We consider four different objective functions for the dual response surface optimization approach. The essence of the proposed method is to reduce the influence of variance of the predicted response by minimizing the variability relative to the quality characteristics of interest and at the same time achieving the specific target output. The basic idea is to convert the constraint optimization function into an unconstraint problem by adding the constraint to the original objective function. Numerical examples and simulations study are carried out to compare performance of the proposed method with some existing procedures. Numerical results show that the performance of the proposed method is encouraging and has exhibited clear improvement over the existing approaches.

  10. Statistical evaluation and modeling of cheap substrate-based cultivation medium of Chlorella vulgaris to enhance microalgae lipid as new potential feedstock for biolubricant.

    Science.gov (United States)

    Mohammad Mirzaie, M A; Kalbasi, M; Mousavi, S M; Ghobadian, B

    2016-05-18

    Chlorella vulgaris (C. vulgaris) microalga was investigated as a new potential feedstock for the production of biodegradable lubricant. In order to enhance microalgae lipid for biolubricant production, mixotrophic growth of C. vulgaris was optimized using statistical analysis of Plackett-Burman (P-B) and response surface methodology (RSM). A cheap substrate-based medium of molasses and corn steep liquor (CSL) was used instead of expensive mineral salts to reduce the total cost of microalgae production. The effects of molasses and CSL concentration (cheap substrates) and light intensity on the growth of microalgae and their lipid content were analyzed and modeled. Designed models by RSM showed good compatibility with a 95% confidence level when compared to the cultivation system. According to the models, optimal cultivation conditions were obtained with biomass productivity of 0.123 g L(-1) day(-1) and lipid dry weight of 0.64 g L(-1) as 35% of dry weight of C. vulgaris. The extracted microalgae lipid presented useful fatty acid for biolubricant production with viscosities of 42.00 cSt at 40°C and 8.500 cSt at 100°C, viscosity index of 185, flash point of 185°C, and pour point of -6°C. These properties showed that microalgae lipid could be used as potential feedstock for biolubricant production. PMID:25844976

  11. Venturini Method Based Matrix Converter

    Directory of Open Access Journals (Sweden)

    Derick Mathew

    2015-03-01

    Full Text Available Recently, matrix converter has received considerable interest as a viable alternative to the conventional ac-dc-ac converter. This direct ac-ac converter provides some attractive characteristics such as: four quadrant operation, absence of bulky dc-link electrolyte capacitors, clean input power characteristics. Due to the absence of dc link energy storage elements any disturbance in the input voltage will be immediately reflected to the output voltages. In this paper venturini method for matrix converter has been presented. Three phase sinusoidal symmetrical voltage or current can obtained .

  12. Spirulina cultivation in China

    Science.gov (United States)

    Wu, Bo-Tang; Xiang, Wen-Zhou; Zeng, Cheng-Kui

    1998-03-01

    This paper reviews and discusses the development and many problems of Spirulina cultivation in China, points out the advantages and disadvantages of open photobioreactor system, and predicts that seawater Spirulina cultivation will be a new trend to be strengthened and emphasized due to its special physiological characteristics, easier management, lower fertilizer cost, and higher resistance to contaminants and rare pollution of chemicals.

  13. An interactive segmentation method based on superpixel

    DEFF Research Database (Denmark)

    Yang, Shu; Zhu, Yaping; Wu, Xiaoyu

    2015-01-01

    This paper proposes an interactive image-segmentation method which is based on superpixel. To achieve fast segmentation, the method is used to establish a Graphcut model using superpixels as nodes, and a new energy function is proposed. Experimental results demonstrate that the authors' method has...... excellent performance in terms of segmentation accuracy and computation efficiency compared with other segmentation algorithm based on pixels....

  14. Digital Autofocusing Method Based on Contourlet Transform

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The autofocusing technique based on contourlet transform is discussed in this paper and an autofocusing method is proposed for images with much information in certain directions. The experimental results show that theproposed method can focus accurately and the sensitivity ratio is higher than that of the other autofocusing methods based on conventional image processing

  15. Satellite-based investigation of flood-affected rice cultivation areas in Chao Phraya River Delta, Thailand

    Science.gov (United States)

    Son, N. T.; Chen, C. F.; Chen, C. R.; Chang, L. Y.

    2013-12-01

    The occurrence of catastrophic floods in Thailand in 2011 caused significant damage to rice agriculture. This study investigated flood-affected rice cultivation areas in the Chao Phraya River Delta (CRD) rice bowl, Thailand using time-series moderate resolution imaging spectroradiometer (MODIS) data. The data were processed for 2008 (normal flood year) and 2011, comprising four main steps: (1) data pre-processing to construct time-series MODIS vegetation indices (VIs), to filter noise from the time-series VIs by the empirical mode decomposition (EMD), and to mask out non-agricultural areas in respect to water-related cropping areas; (2) flood-affected area classification using the unsupervised linear mixture model (ULMM); (3) rice crop classification using the support vector machines (SVM); and (4) accuracy assessment of flood and rice crop mapping results. The comparisons between the flood mapping results and the ground reference data indicated an overall accuracy of 97.9% and Kappa coefficient of 0.62 achieved for 2008, and 95.7% and 0.77 for 2011, respectively. These results were reaffirmed by close agreement (R2 > 0.8) between comparisons of the two datasets at the provincial level. The crop mapping results compared with the ground reference data revealed that the overall accuracies and Kappa coefficients obtained for 2008 were 88.5% and 0.82, and for 2011 were 84.1% and 0.76, respectively. A strong correlation was also found between MODIS-derived rice area and rice area statistics at the provincial level (R2 > 0.7). Rice crop maps overlaid on the flood-affected area maps showed that approximately 16.8% of the rice cultivation area was affected by floods in 2011 compared to 4.9% in 2008. A majority of the flood-expanded area was observed for the double-cropped rice (10.5%), probably due to flood-induced effects to the autumn-summer and rainy season crops. Information achieved from this study could be useful for agricultural planners to mitigate possible impacts

  16. [The Theory and Practice of Health Cultivation Qigong Exercise in Traditional Chinese Medicine].

    Science.gov (United States)

    Chang, Mei-Ying

    2015-12-01

    The health cultivation qigong exercise in traditional Chinese medicine refers to a traditional, integrated method of illness prevention and body strengthening, which promotes the functions of qi and the blood, smooths the meridians (energy channels), and balances the viscera and bowels through the regulation of the mind, the breathing, and the body. The concept of using qi to cultivate human life is part of the health cultivation practices of ancient Chinese codes and of Chinese medicine. This concept includes the principles, methods, essences, and clinical applications of the practice. In addition, traditional health cultivation references the concepts of yinyang, viscera and bowels, qi and blood, meridians, and essential energy spirit theory in order to explain the human biological phenomena, the theoretical and practical perspectives of qigong, and the basis of the treatment principle. The health cultivation qigong exercise of Chinese medicine utilizes the concept of the "unity of nature and human beings" in traditional Chinese thinking in its practice, which emphasizes the conformity to nature and seasons. In order to fully leverage the benefits from the purpose of health cultivation in qigong practice, the priority is to understand the health cultivation mechanism, the essentials/matters, and the precautions of qigong practices. Recently, the evidence regarding both the biological and the psychological benefits of qigong practices have been demonstrated in numbers of research articles. In particular, qigong is currently considered to be one of the best mild exercises that is suited to all age groups. Professional nurses are suggested to include the health cultivation qigong exercise as part of activities that target health improvement and illness prevention. Due to the diversity in qigong as practiced by different health cultivation qigong exercise sects, it is essential to accumulate more clinical evidence by conducting greater numbers of rigorous studies

  17. Comparison and Reference of the Talent Cultivation System in Vocational Education Based on Cultural Creation and Information Industry between Fujian and Taiwan

    Directory of Open Access Journals (Sweden)

    Di Jun An

    2016-01-01

    Full Text Available firstly, the current situation of the cultural creation and information industry in Fujian and Taiwan is investigated and compared in this paper. Secondly, the characteristics and advantages of the talent cultivation system in the cultural creation and information industry of Taiwan are investigated. Finally, the advantages of Taiwanese cultural creation and information industry in talent cultivation are referred to greatly cultivate the talents of cultural creation and information industry in Fujian Province.

  18. Effects of cultivation methods on yield formation of Ningjing 3%栽培方式对宁粳3号产量形成的影响

    Institute of Scientific and Technical Information of China (English)

    王端飞; 耿春苗; 李刚华; 黎泉; 徐晶晶; 王绍华; 丁艳锋

    2011-01-01

    为探讨目前主要的栽培方式对超级稻宁粳3号产量形成的影响,于2009-2010年在江苏省丹阳市宝陵农场南京农业大学试验基地设置机插、撤播、条播、穴播、等距手栽、精量手栽和循环手栽7种栽培方式,比较各种栽培方式的产量及其构成因素、生育进程、水稻干物质积累及运转、穗型和株型特征的差异.结果表明:各处理产量表现由高到低依次为精量手栽、机插、等距手栽、撒播、循环手栽、穴播、条播.手栽高产的优势在于穗型较大,颖花数多;直播稻的劣势是每穗粒数少,颖花数较少;机插每穗粒数和颖花数介于手栽与直播之间.3种手栽方式相比较,循环手栽方式每穗粒数与颖花数明显不足,而等距手栽每穗粒数相对较少,精量手栽方式优势明显.直播方式营养生长期明显缩短,前期干物质积累过少,穗型较小,顶4叶叶片短小,产量较低.相对撒播,条播与穴播方式穗粒数较高,通过适宜的密度配置,增加穗数,有较大的增产潜力.与精量手栽相比,机插前期干物质积累较少,穗型小,提高前期干物质积累,主攻大穗,是实现高产的有效途径.%To study the effects of popular cultivation methods on yield formation in japonica rice Ningjing 3. In 2009-2010,field experiment was conducted in Baoling farm,experiment area of Nanjing Agricultural University, Danyang City,Jiangsu Province. The grain yield and its components, growth process,dry matter, panicle and plant type were analyzed in different cultivations such as machine transplanting (MT), broadcast seeding ( BS), drill seeding ( DS ) , hole-drop seeding ( HS), equidistance hand transplanting (ET) .precise hand transplanting (FT) and cycle hand transplanting(CT). The results showed that the grain yield of PT was the highest,MT was 2nd,ET was 3rd,BS was 4th,CT was 5th,HS was 6th,and DS was the lowest among these treatments. The panicle type and spikelets of hand

  19. The Physical Location of Gm-2 and Gm-6 in O.officinalis with BAC-FISH Based on Comparative RFLP Map of Wild Rice and Cultivated Rice

    Institute of Scientific and Technical Information of China (English)

    QIN Rui; WEI Wen-hui; NING Shun-bin; JIN Wei-wei; HE Guang-cun; SONG Yun-chun

    2002-01-01

    Rice BAC library is used widely in rice genome research due to its distinctive advantages over other library systems. In this study, two rice BAC clones closely linked to rice gall midge resistance, Gm-2 and Gm-6, were in situ hybridized to Oryza officinalis chromosomes. They were located on the long arm of chromosome 4 with FL 72.33% and 77.10% respectively and their FL was consistent with the selective marker of rice, RG214 and RZ569. The frequency of signal detection was 61.2% and 59.5% respectively.Our study was based on comparative RFLP map of wild rice, O. officinalis, and cultivated rice, O. sativa.

  20. DNA-based taxonomic identification of basidiospores in hallucinogenic mushrooms cultivated in "grow-kits" seized by the police: LC-UV quali-quantitative determination of psilocybin and psilocin.

    Science.gov (United States)

    Gambaro, Veniero; Roda, Gabriella; Visconti, Giacomo Luca; Arnoldi, Sebastiano; Casagni, Eleonora; Dell'Acqua, Lucia; Farè, Fiorenza; Paladino, Eleonora; Rusconi, Chiara; Arioli, Stefania; Mora, Diego

    2016-06-01

    The taxonomic identification of the biological material contained in the hallucinogenic mushrooms culture media, was carried out using a DNA-based approach, thus highlighting the usefulness of this approach in the forensic identification of illegal samples also when they are present as basidiospores mixed in culture media and spore-bearing fruiting body are not present. This approach is very useful as it allows the unequivocal identification of potentially illicit material before the cultivation and it enables to stop the material to the Customs and to destroy it due to its dangerousness without cultivating the "grow-kits" and without instructing a criminal case. In fact, even if psilocin and psilocybin and the whole mushrooms are illegal in many countries, there is no specific indication in the law about the so called "grow-kits", containing the spores. To confirm the data obtained by the taxonomic identification, a simple, reliable, efficient LC-UV method, using tryptamine as internal standard, suitable for the forensic quali-quantitative determination of psilocin and psilocybin in hallucinogenic mushroom was optimized, validated and applied to the mushrooms grown after the cultivation of the grow-kits seized by the judicial authority, with the authorization of the Ministry of Health. A cation exchange column was used in a gradient elution mode (Phase A: 50mMK2HPO4; 100mM NaCl pH=3 Phase B: methanol). The developed method was linear over the calibration range with a R(2)>0.9992 for both the analytes. The detection and quantification limits were respectively 0.01 and 0.1μg/mL for psilocybin and 0.05μg/mL and 0.1μg/mL for psilocin and the intra- and inter-day precision was satisfactory (coefficients of variation <2.0% for both the analytes). The content of psilocybin in the mushrooms grown from the seized "grow-kits" ranged from 1.02 to 7.60mg/g of dry vegetable material, while the content of psilocin from 0.415 to 8.36mg/g. PMID:27021629

  1. Development of a method for the control of an environmentally friendly cultivation of energy crops at provincial level using a differentiated premium payment; Entwicklung einer Methodik zur Steuerung eines umweltschonenden Energiepflanzenanbaus auf Landesebene durch eine differenzierte Praemienzahlung

    Energy Technology Data Exchange (ETDEWEB)

    Greiff, Kathrin Britta

    2012-07-01

    Facing the global challenge of climate change and finiteness of fossil resources renewable resources are becoming more and more important at present. At the moment, biomass is the most important renewable resource in Germany. The rising cultivation of energy crops, supported by political instruments, has a negative environmental impact on the natural resources soil, water and biodiversity. The existing agricultural legislation can not avoid such negative environmental impact. Thus, it seems reasonable to realign the existing support scheme for energy crop use to a system that operates regional and crop specific but includes the whole agricultural sector as well. Such a newly designed system could be realised by a differentiated premium. This instrument is most suitable to prevent environmental damage due to the advantages of a regional use and a differentiation of crop species. The aim of this work has been to identify an easy applicable and transferable control mechanism for an environmental friendly support scheme of energy crops and also to develop and test a method as basis for this control mechanism. The design of a regional and crop specific premium has been described as a possible political instrument. The method has been developed and tested within a case study for Bavaria, one of Germany's federal states. For the investigation the level of agrarian production regions has been chosen. For each region it has been assessed which crops should and which ones should not be cultivated. For this, the actual environmental risk for the agricultural activity has been calculated by using landscape analysis on the basis of environmental risk assessment. Agrarian statistics (InVeKos) and digital GIS-based site data has been used as data base. With this data base it has been possible to illustrate the environmental risk for the natural resources soil, water and biodiversity in sufficient exactness. The comparison of the periods 1994-96 and 2005-07 approved that the

  2. Design for the Cultivation of Employment Competence of Higher Vocational Students Based on the Competency%基于胜任特征的高职生就业能力培养设计*

    Institute of Scientific and Technical Information of China (English)

    王小明

    2013-01-01

    本文介绍了基于高职生成功就业胜任特征模型的高职生就业能力培养设计的研究,提出胜任特征水平测试、鉴定胜任特征差距、规划胜任特征差距、自我指导的行动学习、评估行动学习结果等五个环节的基于成功就业胜任特征的自我指导的行动学习法,列举基于胜任特征就业能力培养的需求分析所需胜任特征目标和评价标准。然后提出了基于胜任特征高职生就业能力培养时应当注意的一些问题和思考。%This paper introduces the research of cultivation of employment competency of higher vocational students based on competency model, and proposes self-instructed action learning methods, which are composed of five links:the competency level test, identification of the competency gap, planning the competency gap, self-instructed action learning and evaluation of action learning re-sults. And it also lists the target and evaluation standard of competency based on requirement analysis. At last, it puts forward some problems and reflections on cultivation of employment competency.

  3. Analysis of agronomic and domestication traits in a durum x cultivated emmer wheat population using a high-density single nucleotide polymorphism-based linkage map

    Science.gov (United States)

    Cultivated emmer wheat (Triticum turgidum ssp. dicoccum) is tetraploid and considered one of the eight founder crops that spawned the Agricultural Revolution about 10,000 years ago. Cultivated emmer has non-free-threshing seed and a somewhat fragile rachis, but mutations in genes governing these an...

  4. Molecular- and cultivation-based analyses of microbial communities in oil field water and in microcosms amended with nitrate to control H{sub 2}S production

    Energy Technology Data Exchange (ETDEWEB)

    Kumaraswamy, Raji; Ebert, Sara; Fedorak, Phillip M.; Foght, Julia M. [Alberta Univ., Edmonton, AB (Canada). Biological Sciences; Gray, Murray R. [Alberta Univ., Edmonton, AB (Canada). Chemical and Materials Engineering

    2011-03-15

    Nitrate injection into oil fields is an alternative to biocide addition for controlling sulfide production ('souring') caused by sulfate-reducing bacteria (SRB). This study examined the suitability of several cultivation-dependent and cultivation-independent methods to assess potential microbial activities (sulfidogenesis and nitrate reduction) and the impact of nitrate amendment on oil field microbiota. Microcosms containing produced waters from two Western Canadian oil fields exhibited sulfidogenesis that was inhibited by nitrate amendment. Most probable number (MPN) and fluorescent in situ hybridization (FISH) analyses of uncultivated produced waters showed low cell numbers ({<=}10{sup 3} MPN/ml) dominated by SRB (>95% relative abundance). MPN analysis also detected nitrate-reducing sulfide-oxidizing bacteria (NRSOB) and heterotrophic nitrate-reducing bacteria (HNRB) at numbers too low to be detected by FISH or denaturing gradient gel electrophoresis (DGGE). In microcosms containing produced water fortified with sulfate, near-stoichiometric concentrations of sulfide were produced. FISH analyses of the microcosms after 55 days of incubation revealed that Gammaproteobacteria increased from undetectable levels to 5-20% abundance, resulting in a decreased proportion of Deltaproteobacteria (50-60% abundance). DGGE analysis confirmed the presence of Delta- and Gammaproteobacteria and also detected Bacteroidetes. When sulfate-fortified produced waters were amended with nitrate, sulfidogenesis was inhibited and Deltaproteobacteria decreased to levels undetectable by FISH, with a concomitant increase in Gammaproteobacteria from below detection to 50-60% abundance. DGGE analysis of these microcosms yielded sequences of Gamma- and Epsilonproteobacteria related to presumptive HNRB and NRSOB (Halomonas, Marinobacterium, Marinobacter, Pseudomonas and Arcobacter), thus supporting chemical data indicating that nitrate-reducing bacteria out-compete SRB when nitrate is

  5. Cultivation-Based and Molecular Assessment of Bacterial Diversity in the Rhizosheath of Wheat under Different Crop Rotations.

    Directory of Open Access Journals (Sweden)

    Muhammad Tahir

    Full Text Available A field study was conducted to compare the formationand bacterial communities of rhizosheaths of wheat grown under wheat-cotton and wheat-rice rotation and to study the effects of bacterial inoculation on plant growth. Inoculation of Azospirillum sp. WS-1 and Bacillus sp. T-34 to wheat plants increased root length, root and shoot dry weight and dry weight of rhizosheathsoil when compared to non-inoculated control plants, and under both crop rotations. Comparing both crop rotations, root length, root and shoot dry weight and dry weight of soil attached with roots were higher under wheat-cotton rotation. Organic acids (citric acid, malic acid, acetic acid and oxalic acid were detected in rhizosheaths from both rotations, with malic acid being most abundant with 24.8±2 and 21.3±1.5 μg g(-1 dry soil in wheat-cotton and wheat-rice rotation, respectively. Two sugars (sucrose, glucose were detected in wheat rhizosheath under both rotations, with highest concentrations of sucrose (4.08±0.5 μg g(-1 and 7.36±1.0 μg g(-1 and glucose (3.12±0.5 μg g(-1 and 3.01± μg g(-1 being detected in rhizosheaths of non-inoculated control plants under both rotations. Diversity of rhizosheath-associated bacteria was evaluated by cultivation, as well as by 454-pyrosequencing of PCR-tagged 16S rRNA gene amplicons. A total of 14 and 12 bacterial isolates predominantly belonging to the genera Arthrobacter, Azospirillum, Bacillus, Enterobacter and Pseudomonaswere obtained from the rhizosheath of wheat grown under wheat-cotton and wheat-rice rotation, respectively. Analysis of pyrosequencing data revealed Proteobacteria, Bacteriodetes and Verrucomicrobia as the most abundant phyla in wheat-rice rotation, whereas Actinobacteria, Firmicutes, Chloroflexi, Acidobacteria, Planctomycetes and Cyanobacteria were predominant in wheat-cotton rotation. From a total of 46,971 sequences, 10.9% showed ≥97% similarity with 16S rRNA genes of 32 genera previously shown to include

  6. Cultivation-Based and Molecular Assessment of Bacterial Diversity in the Rhizosheath of Wheat under Different Crop Rotations.

    Science.gov (United States)

    Tahir, Muhammad; Mirza, M Sajjad; Hameed, Sohail; Dimitrov, Mauricio R; Smidt, Hauke

    2015-01-01

    A field study was conducted to compare the formationand bacterial communities of rhizosheaths of wheat grown under wheat-cotton and wheat-rice rotation and to study the effects of bacterial inoculation on plant growth. Inoculation of Azospirillum sp. WS-1 and Bacillus sp. T-34 to wheat plants increased root length, root and shoot dry weight and dry weight of rhizosheathsoil when compared to non-inoculated control plants, and under both crop rotations. Comparing both crop rotations, root length, root and shoot dry weight and dry weight of soil attached with roots were higher under wheat-cotton rotation. Organic acids (citric acid, malic acid, acetic acid and oxalic acid) were detected in rhizosheaths from both rotations, with malic acid being most abundant with 24.8±2 and 21.3±1.5 μg g(-1) dry soil in wheat-cotton and wheat-rice rotation, respectively. Two sugars (sucrose, glucose) were detected in wheat rhizosheath under both rotations, with highest concentrations of sucrose (4.08±0.5 μg g(-1) and 7.36±1.0 μg g(-1)) and glucose (3.12±0.5 μg g(-1) and 3.01± μg g(-1)) being detected in rhizosheaths of non-inoculated control plants under both rotations. Diversity of rhizosheath-associated bacteria was evaluated by cultivation, as well as by 454-pyrosequencing of PCR-tagged 16S rRNA gene amplicons. A total of 14 and 12 bacterial isolates predominantly belonging to the genera Arthrobacter, Azospirillum, Bacillus, Enterobacter and Pseudomonaswere obtained from the rhizosheath of wheat grown under wheat-cotton and wheat-rice rotation, respectively. Analysis of pyrosequencing data revealed Proteobacteria, Bacteriodetes and Verrucomicrobia as the most abundant phyla in wheat-rice rotation, whereas Actinobacteria, Firmicutes, Chloroflexi, Acidobacteria, Planctomycetes and Cyanobacteria were predominant in wheat-cotton rotation. From a total of 46,971 sequences, 10.9% showed ≥97% similarity with 16S rRNA genes of 32 genera previously shown to include isolates

  7. Cultivation-Based and Molecular Assessment of Bacterial Diversity in the Rhizosheath of Wheat under Different Crop Rotations

    Science.gov (United States)

    Tahir, Muhammad; Mirza, M. Sajjad; Hameed, Sohail; Dimitrov, Mauricio R.; Smidt, Hauke

    2015-01-01

    A field study was conducted to compare the formationand bacterial communities of rhizosheaths of wheat grown under wheat-cotton and wheat-rice rotation and to study the effects of bacterial inoculation on plant growth. Inoculation of Azospirillum sp. WS-1 and Bacillus sp. T-34 to wheat plants increased root length, root and shoot dry weight and dry weight of rhizosheathsoil when compared to non-inoculated control plants, and under both crop rotations. Comparing both crop rotations, root length, root and shoot dry weight and dry weight of soil attached with roots were higher under wheat-cotton rotation. Organic acids (citric acid, malic acid, acetic acid and oxalic acid) were detected in rhizosheaths from both rotations, with malic acid being most abundant with 24.8±2 and 21.3±1.5 μg g-1 dry soil in wheat-cotton and wheat-rice rotation, respectively. Two sugars (sucrose, glucose) were detected in wheat rhizosheath under both rotations, with highest concentrations of sucrose (4.08±0.5 μg g-1and 7.36±1.0 μg g-1) and glucose (3.12±0.5 μg g-1 and 3.01± μg g-1) being detected in rhizosheaths of non-inoculated control plants under both rotations. Diversity of rhizosheath-associated bacteria was evaluated by cultivation, as well as by 454-pyrosequencing of PCR-tagged 16S rRNA gene amplicons. A total of 14 and 12 bacterial isolates predominantly belonging to the genera Arthrobacter, Azospirillum, Bacillus, Enterobacter and Pseudomonaswere obtained from the rhizosheath of wheat grown under wheat-cotton and wheat-rice rotation, respectively. Analysis of pyrosequencing data revealed Proteobacteria, Bacteriodetes and Verrucomicrobia as the most abundant phyla in wheat-rice rotation, whereas Actinobacteria, Firmicutes, Chloroflexi, Acidobacteria, Planctomycetes and Cyanobacteria were predominant in wheat-cotton rotation. From a total of 46,971 sequences, 10.9% showed ≥97% similarity with 16S rRNA genes of 32 genera previously shown to include isolates with plant

  8. Advancing gut microbiome research using cultivation

    DEFF Research Database (Denmark)

    Sommer, Morten OA

    2015-01-01

    Culture-independent approaches have driven the field of microbiome research and illuminated intricate relationships between the gut microbiota and human health. However, definitively associating phenotypes to specific strains or elucidating physiological interactions is challenging for metagenomic...... approaches. Recently a number of new approaches to gut microbiota cultivation have emerged through the integration of high-throughput phylogenetic mapping and new simplified cultivation methods. These methodologies are described along with their potential use within microbiome research. Deployment of novel...

  9. A study on the comparison of antioxidant effects among cultivated ginseng, and cultivated wild ginseng extracts -Using the measurement of superoxide and hydroxy radical scavenging activities-

    Directory of Open Access Journals (Sweden)

    Tae Jin, Rhim

    2009-06-01

    Full Text Available Objectives : The objective of this study was to compare the antioxidant effects among cultivated wild ginseng and ginseng extracts. Methods : In vitro antioxidant activities were examined by superoxide and hydroxyl radical scavenging activities of ginseng and cultivated wild ginseng extracts. Results : 1. In the superoxide radical scavenging activities of ginseng and cultivated wild ginseng extracts, antioxidant activities of cultivated wild ginseng extracts was showed higher than cultivated ginseng in the concentration of 0.25 and 0.50㎎/㎖. 2. In the hydroxyl radical scavenging activities of ginseng and cultivated wild ginseng extracts, antioxidant activities of cultivated wild ginseng extracts was showed higher than cultivated ginseng in the concentration of 1.0, 2.5, and 5.0㎎/㎖. Conclusions : In summary, the results of this study demonstrate that cultivated wild ginseng extracts had higher antioxidant activities to cultivated ginseng.

  10. Cultivated Land Changes and Their Driving Forces-A Satellite Remote Sensing Analysis in the Yellow River Delta, China

    Institute of Scientific and Technical Information of China (English)

    ZHAO Geng-Xing; G.LIN; J.J.FLETCHER; C.YUILL

    2004-01-01

    Taking Kenli County in the Yellow River Delta, China, as the study area and using digital satellite remote sensing techniques, cultivated land use changes and their corresponding driving forces were explored in this study. An interactive interpretation and a manual modification procedure were carried out to acquire cultivated land information. An overlay method based on classification results and a visual change detection method which was supported by land use maps were employed to detect the cultivated land changes. Based on the changes that were revealed and a spatial analysis between cultivated land use and related natural and socio-economic factors, the driving forces for cultivated land use changes in the study area were determined.The results showed a decrease in cultivated land in Kenli County of 5321.8 ha from 1987 to 1998, i.e.,an average annual decrement of 483.8 ha, which occurred mainly in the central paddy field region and the northeast dry land region. Adverse human activities, soil salinization and water deficiencies were the driving forces that caused these cultivated land use changes.

  11. 土地利用现状调查资料在耕地地力评价中的应用%Application research of cultivated land fertility evaluation based on land use status data

    Institute of Scientific and Technical Information of China (English)

    蒋玉根; 吴豪翔; 裘希雅; 白金河; 何旭华; 陈侃

    2011-01-01

    以1∶10 000土地利用现状图为空间数据框架基础,结合农业部门现有的土壤普查和样点调查分析资料进行耕地地力评价,相比目前我国测土配方施肥工作方案中以1∶50 000为基础进行的耕地地力评价结果,本研究方法的评价结果耕地分布的空间定位更正确、更切合现状,评价单元的划分更合理、耕地地力等级划分更加精细,能大幅度提高耕地地力评价的精度.本研究方法在耕地地力评价上的应用,有利于为建立测土配方施肥信息管理系统提供详细的基础信息和科学管理耕地,更好地满足精准农业和农业生产中施肥管理对耕地信息的需要.%The purpose of this paper was to evaluate cultivated land fertility based on land use status map at 1- 10 000 scale combined with existing soil survey and soil sample analysis data. The evaluation results showed that spatial positioning of cultivated land distribution was a more accurate and practical situation and that the assessment unit division of cultivated land classification was more reasonable and more sophisticated, and the evaluation accuracy was greatly improved, compared to evaluation at 1:50 000 scale implemented in the work program of testing soil for formulated fertilization in our country. The research method in this paper was beneficial for the establishment of soil testing and formulated fertilization information management systems to provide detailed basic information and scientific management of farmland. It can better meet farmland information needs on precision agriculture and fertilizer management for agricultural production.

  12. Reliability-based concurrent subspace optimization method

    Institute of Scientific and Technical Information of China (English)

    FAN Hui; LI Wei-ji

    2008-01-01

    To avoid the high computational cost and much modification in the process of applying traditional re-liability-based design optimization method, a new reliability-based concurrent subspace optimization approach is proposed based on the comparison and analysis of the existing muhidisciplinary optimization techniques and reli-ability assessment methods. It is shown through a canard configuration optimization for a three-surface transport that the proposed method is computationally efficient and practical with the least modification to the current de-terministic optimization process.

  13. Resposta do milho a fontes e modos de aplicação de fósforo durante três cultivos sucessivos em solo da região do Cerrado Maize response to phosphorus sources and application methods during three successive cultivations in soil of the Cerrado region

    Directory of Open Access Journals (Sweden)

    Álvaro Vilela de Resende

    2006-06-01

    Full Text Available Objetivou-se comparar fertilizantes fosfatados em diferentes modos de aplicação, durante três cultivos sucessivos de milho, num Argissolo Vermelho já adubado anteriormente. Foi utilizado um fatorial 4 x 2+1, envolvendo quatro fontes de P (superfosfato triplo ST, termofosfato magnesiano TM, fosfato reativo de Arad FR e fosfato natural de Araxá FA, duas formas de aplicação (a lanço ou no sulco de plantio, e uma testemunha (sem P como tratamento adicional. Foram fornecidos 180 kg ha-1 de P2O5 no primeiro cultivo, com base nos teores totais das fontes. Para os cultivos seguintes, não foi feito preparo do solo. Determinaram-se os teores de nutrientes no solo e nas folhas de milho e a produção de grãos. As respostas aos tratamentos foram mais discrepantes inicialmente e tenderam à eqüidade com os cultivos sucessivos. Nas duas primeiras safras, as fontes de maior solubilidade (ST e TM ocasionaram as maiores produções. Os fosfatos naturais (FR e FA apresentaram aumento de eficiência com o tempo. A aplicação localizada do FR proporcionou alta produtividade na terceira safra. O residual de antigas adubações e as condições climáticas influenciaram os efeitos dos tratamentos.The objective of this study was to compare phosphate fertilizers in different methods of application during three successive maize cultivations in a Red Argisol (Hapludults which had been previously fertilized. Treatments arranged in a 4x2+1 factorial design combined four P sources in the amount of 180 kg ha-1 P2O5 (triple superphosphate - TS, magnesium termophosphate - MT, Arad reactive rock phosphate - RP, and Araxá rock phosphate - AP, two application methods (broadcasted or banded in the seeding furrow, and a check plot (without P as an additional treatment. The applied P ratio was based on the total P2O5 content of each fertilizer. After the first cultivation, the soil was no longer revolved. Soil and leaf nutrient concentrations, and the grain yield were

  14. Exploration on the Cultivation of Gray Collar Talents Based on the Perspective of Internal Career%基于内职业生涯角度探讨灰领人才的培养

    Institute of Scientific and Technical Information of China (English)

    陈伟

    2015-01-01

    Currently, there is a vicious cycle of talent supply in the job market, that is,"students have difficulties in employment and enterprises have difficulties in recruitment", and gray collar, as a new career field, will be the key to solving the paradox be-tween university students' employment problems and China's tal-ent supply. Starting from the grim employment situation of uni-versity students and the shortage of gray collar talents, this paper analyzes university students' abandonment of "gray collar" and dilemmas in employment due to their obsolete outlooks on em-ployment, and based on the perspective of internal career, finds methods to establish university students' new outlooks on em-ployment and cultivate gray collar talents, thus arousing the pon-dering on the environment for gray collar cultivation in China.%当前,我国就业市场上存在着“学生就业难、企业招工难”的人才供给的怪圈,而灰领作为一种新的职业领域,将成为解决大学生就业问题和我国人才供给悖论的关键所在。本文从我国大学生就业的严峻形势和灰领人才的紧缺问题着手,对大学生因陈旧就业观而放弃“灰领”、就业两难的成因进行分析,从而基于内职业生涯的角度,找出树立大学生新型就业观、打造灰领人才的方法,同时,引出高校对我国灰领培育环境的思考。

  15. 浅谈培养学生音乐记忆力的方法与途径%A Brief Discussion on the Methods and Approaches of Cultivating Students' Music Memory

    Institute of Scientific and Technical Information of China (English)

    张建芬

    2013-01-01

    音乐记忆力指的是对音乐作品的记忆能力,也是我们学生学习音乐必须具备的基本能力,正确的音乐记忆应遵循记忆的规律,讲究方法,在学习中加以培养。本文旨在如何培养学生良好的音乐记忆能力上做了一些尝试。%Music memory, students' basic ability in learning mu-sic, is the memorizing ability of music works. A correct music memory obeys the rules and methods of memory and should be cultivated in learning. This paper attempts to cultivate students' correct music memory ability.

  16. Spatial distribution of prime farmland based on cultivated land quality comprehensive evaluation at county scale%基于耕地质量综合评价的县域基本农田空间布局

    Institute of Scientific and Technical Information of China (English)

    奉婷; 张凤荣; 李灿; 曲衍波; 朱凤凯

    2014-01-01

    For the cultivated land quality comprehensive evaluation there have been relatively abundant research results at present, but most of them tend to judge the suitability of spatial distribution of cultivated land and prime farmland without taking sufficient attention to the ecological security. The cultivated land quality comprehensive evaluation plays an important part as prerequisites and groundwork in demarcating of prime farmland and high-standard prime farmland construction. On the basis of dissecting the concept and method of cultivated land quality evaluation, the paper constructed a cultivated quality comprehensive evaluation system by way of selecting relevant indicators from the natural quality, site conditions, spatial pattern and ecological security of cultivated land through referring to agricultural land classification, agricultural land gradation, cultivated land scale operation and its ecological security. The study aimed at meeting the inner demands for demarcating of prime farmland and made up for the deficiency in the present related researches, then achieved quantitative analysis of various indexes and calculation on the cultivated land quality comprehensive index with the help of GIS. On the above basis, the study divided 5 916 cultivated land parcels into 4 different types according to the similarity theory in Pinggu district:1) The first one, named as the prior planning type, was up to 4 325.29 hm2 and accounted for 35.06% of regional total cultivated land area, distributed mainly in the western, southern and southwest plains of Pinggu district, mainly in the town of Donggaocun, followed by the towns of Yukou and Mafang, which showed comprehensive optimum in the natural quality, site conditions, ecological security and spatial pattern of cultivated land as the preferred object to be prime farmland or high-standard prime farmland. This type became the core part of cultivated land protected in Pinggu district. 2) The second one, named as the

  17. Discrete mechanics Based on Finite Element Methods

    OpenAIRE

    Chen, Jing-Bo; Guo, Han-Ying; Wu, Ke

    2002-01-01

    Discrete Mechanics based on finite element methods is presented in this paper. We also explore the relationship between this discrete mechanics and Veselov discrete mechanics. High order discretizations are constructed in terms of high order interpolations.

  18. Soilless cultivation system for functional food crops

    International Nuclear Information System (INIS)

    This soilless cultivation system is based on the fertigation system and cultivation technologies using Functional Plant Cultivation System (FPCS). EBARA Japan has been studying on the cultivation conditions in order to enhance the function of decease risk reduction in plants. Through the research and development activities, EBARA found the possibilities on the enhancement of functions. Quality and quantity of the products in term of bioactive compounds present in the plants may be affected by unforeseen environmental conditions, such as temperature, strong light and UV radiation. The main objective to develop this system is, to support? Functional Food Industry? as newly emerging field in agriculture business. To success the system, needs comprehensive applying agriculture biotechnologies, health biotechnologies and also information technologies, in agriculture. By this system, production of valuable bioactive compounds is an advantage, because the market size of functional food is increasing more and more in the future. (Author)

  19. 袁同礼的图书馆人才培植方法及启示%Yuan Tongli's Methods about Cultivating Talents of Libraries and Its Revelation

    Institute of Scientific and Technical Information of China (English)

    潘梅

    2011-01-01

    Yuan Tongli who is an important leader of Chinese librarianship in the period of the Republic of China, he has own unique ways of cultivating talents. In selecting talents, he attached importance to knowledge basis and academic skills of talents. In using talents, he respected talents' interests and took advantage of their strengths. In cultivating talents, he provided many opportunities of academic training and advanced studying for talents. In retaining talents, he cared them and fully played leadership charisma. Yuan Tongli's methods about cultivating talents have a great inspiration for libraries today. Firstly, cultivating talents of library is a strategic issue related to librarianship development and academic progress. Secondly, libraries should moderately increase the space-level positions in favor to cultivate talents. Finally, libraries should pay attention to the establishment of long-term mechanism of cultivating talents.%民国时期中国图书馆事业的重要领导者袁同礼在培植图书馆人才上有自己独特的方法:在选才方面,重视人才的知识基础和学术功底;在用才方面,尊重人才的志趣,用人所长;在育才方面,努力为人才提供学术训练和进修深造的机会,注重人才的成长;在留才方面,关心人才,充分发挥个人的领导魅力。此方法对当今图书馆的人才培植仍有极大的启发意义。图书馆人才培养是事关图书馆事业发展、学术进步的战略问题,图书馆应适度增加空间职位层次,建立长效的人才培养机制,为人才提供多方面的成长渠道。

  20. Cost effective and economic method for cultivation of Chlorella pyrenoidosa for the simultaneous treatment of biogas digester wastewater and biogas production

    OpenAIRE

    Rohit Sharma; Avanish K Tiwari; G. Sanjay Kumar; Bhawna Y. Lamba

    2015-01-01

    Microalgae have recently received a lot of attention as a new biomass source for the production of bio fuels and for the treatment of waste water. In this work, Chlorella pyrenoidosa was cultivated in biogas digester wastewater. The growth kinetics of the algae as well as the bio-remediation effect on the waste water was studied. The Chlorella pyrenoidosa can utilize the nitrogen content present in biogas digester wastewater as a substrate for its growth. The growth of microalgae was found to...

  1. Agent Based Image Segmentation Method : A Review

    OpenAIRE

    Pooja Mishra; Navita Srivastava; Shukla, K. K.; Achintya Singlal

    2011-01-01

    Image segmentation is an important research area in computer vision and many segmentation methods have been proposed. This paper attempts to provide a brief overview of elemental segmentation techniques based on boundary or regional approaches. It focuses mainly on the agent based image segmentation techniques

  2. Design for validation, based on formal methods

    Science.gov (United States)

    Butler, Ricky W.

    1990-01-01

    Validation of ultra-reliable systems decomposes into two subproblems: (1) quantification of probability of system failure due to physical failure; (2) establishing that Design Errors are not present. Methods of design, testing, and analysis of ultra-reliable software are discussed. It is concluded that a design-for-validation based on formal methods is needed for the digital flight control systems problem, and also that formal methods will play a major role in the development of future high reliability digital systems.

  3. Model-Based Method for Sensor Validation

    Science.gov (United States)

    Vatan, Farrokh

    2012-01-01

    Fault detection, diagnosis, and prognosis are essential tasks in the operation of autonomous spacecraft, instruments, and in situ platforms. One of NASA s key mission requirements is robust state estimation. Sensing, using a wide range of sensors and sensor fusion approaches, plays a central role in robust state estimation, and there is a need to diagnose sensor failure as well as component failure. Sensor validation can be considered to be part of the larger effort of improving reliability and safety. The standard methods for solving the sensor validation problem are based on probabilistic analysis of the system, from which the method based on Bayesian networks is most popular. Therefore, these methods can only predict the most probable faulty sensors, which are subject to the initial probabilities defined for the failures. The method developed in this work is based on a model-based approach and provides the faulty sensors (if any), which can be logically inferred from the model of the system and the sensor readings (observations). The method is also more suitable for the systems when it is hard, or even impossible, to find the probability functions of the system. The method starts by a new mathematical description of the problem and develops a very efficient and systematic algorithm for its solution. The method builds on the concepts of analytical redundant relations (ARRs).

  4. Optimization of Milk-Based Medium for Efficient Cultivation of Bifidobacterium pseudocatenulatum G4 Using Face-Centered Central Composite-Response Surface Methodology

    OpenAIRE

    2014-01-01

    This study was undertaken to optimize skim milk and yeast extract concentration as a cultivation medium for optimal Bifidobacteria pseudocatenulatum G4 (G4) biomass and β -galactosidase production as well as lactose and free amino nitrogen (FAN) balance after cultivation period. Optimization process in this study involved four steps: screening for significant factors using 23 full factorial design, steepest ascent, optimization using FCCD-RSM, and verification. From screening steps, skim milk...

  5. 培育人才创新动力:知识型服务业发展的关键%Cultivating Talents as the Power of Innovation:the Key to the Development of Knowledge-based Service Industry

    Institute of Scientific and Technical Information of China (English)

    王凌

    2013-01-01

    Accelerating the development of knowledge-based service industry ought to be the strategic choice of China’s economic restructuring and development. The cultivation of talent innovation impetus determines the development pace of knowledge-based service industry for a period of time in future. The impetuses for talents in mental and material basis, career development,cultural values and external coordination are the major factors that influence the effect of cultivation.Playing the“enhancing,regulating and guiding”role during such cultivation will be the specific duties of the government as an important cultivation subject.%加快发展知识型服务业是中国经济转型发展的战略选择,行业人才创新动力的培育决定着今后一段时期知识型服务业的加快发展程度。人才心智动力、物质基础动力、职业发展动力、文化价值动力、组织外部动力是影响培育成效的重要因素,发挥好政府在培育中的“充实、规范、引导”作用则是政府作为培育重要主体的具体职责实现。

  6. Software Testing Method Based on Model Comparison

    Institute of Scientific and Technical Information of China (English)

    XIE Xiao-dong; LU Yan-sheng; MAO Cheng-yin

    2008-01-01

    A model comparison based software testing method (MCST) is proposed. In this method, the requirements and programs of software under test are transformed into the ones in the same form, and described by the same model describe language (MDL).Then, the requirements are transformed into a specification model and the programs into an implementation model. Thus, the elements and structures of the two models are compared, and the differences between them are obtained. Based on the diffrences, a test suite is generated. Different MDLs can be chosen for the software under test. The usages of two classical MDLs in MCST, the equivalence classes model and the extended finite state machine (EFSM) model, are described with example applications. The results show that the test suites generated by MCST are more efficient and smaller than some other testing methods, such as the path-coverage testing method, the object state diagram testing method, etc.

  7. 基于跨文化交际能力培养的大学英语教学模式探究*%Exploration on College English Teaching Model Based on Cultivating Cross-cultural Communication Ability

    Institute of Scientific and Technical Information of China (English)

    糜艳庆

    2013-01-01

    With economic globalization promoting cultural globalization,the cultural diversity of the world strongly impacts the talents cultivation mode of higher education in China.Internationalization should be-come an important direction of the Chinese higher education development in the future.College English teaching should not only emphasize the cultivation of the students’basic language skills,but should focus on an international and cross-cultural concept to cultivate internationalized talents with international vision and thorough understanding of international rules.The core issues of college English teaching model based on cultivating cross-cultural communication ability are teaching concepts,curriculum and teaching methods reform.The concept of multiculturalism and the two-way cross-cultural communication should be estab-lished and cultural hegemony and the loss of Chinese culture should be alerted as well.The curriculum sys-tem should be established including the core curriculum (college English),English curriculum for special purposes (ESP)and elective curriculum (culture and communication).The classroom teaching mode of Chinese teachers in collaboration with foreign teachers teaching should be enforced.%经济全球化带动文化全球化,世界文化的多样性强烈冲击着我国高等教育的人才培养模式。“国际化”应该成为今后我国高等教育发展的一个重要方向。对于大学英语来说,其教学不能只关注学生基础语言技能的培养,而要“以面向国际和跨文化的理念为中心”,为培养具有国际视野和通晓国际规则的国际化人才服务;基于跨文化交际能力培养的大学英语教学模式的核心问题是教学理念、课程设置和教学方法的改革;树立多元文化及双向跨文化交际的理念,警惕文化霸权主义及中国文化缺失;建立由核心课程(大学英语)、专业课程(ESP)和选修课程(文化与交际)构成的课

  8. Cultivating Common Ground: Integrating standards-based visual arts, math and literacy in high-poverty urban classrooms

    OpenAIRE

    Cunnington, Marisol; Kantrowitz, Andrea; Harnett, Susanne; Hill-Ries, Aline

    2014-01-01

    The Framing Student Success: Connecting Rigorous Visual Arts, Math and Literacy Learning experimental demonstration project was designed to develop and test an instructional program integrating high-quality, standards-based instruction in the visual arts, math, and literacy. Developed and implemented by arts-in-education organization Studio in a School (STUDIO), in partnership with the New York City Department of Education, the Framing Student Success curriculum was designed by e...

  9. Influence of carvacrol and 1,8-cineole on cell viability, membrane integrity, and morphology of Aeromonas hydrophila cultivated in a vegetable-based broth.

    Science.gov (United States)

    de Sousa, Jossana Pereira; de Oliveira, Kataryne Árabe Rimá; de Figueiredo, Regina Celia Bressan Queiroz; de Souza, Evandro Leite

    2015-02-01

    This study investigated the effects of carvacrol (CAR) and 1,8-cineole (CIN) alone (at the MIC) or in combination at subinhibitory amounts (both at 1/8 MIC) on the cell viability, membrane permeability, and morphology of Aeromonas hydrophila INCQS 7966 (A. hydrophila) cultivated in a vegetable-based broth. CAR and CIN alone or in combination severely affected the viability of the bacteria and caused dramatic changes in the cell membrane permeability, leading to cell death, as observed by confocal laser microscopy. Scanning and transmission electron microscopy images of bacterial cells exposed to CAR or CIN or the mixture of both compounds revealed severe changes in cell wall structure, rupture of the plasma membrane, shrinking of cells, condensation of cytoplasmic content, leakage of intracellular material, and cell collapse. These findings suggest that CAR and CIN alone or in combination at subinhibitory amounts could be applied to inhibit the growth of A. hydrophila in foods, particularly as sanitizing agents in vegetables. PMID:25710162

  10. iTRAQ-based quantitative proteomic analysis reveals proteomic changes in leaves of cultivated tobacco (Nicotiana tabacum) in response to drought stress.

    Science.gov (United States)

    Xie, He; Yang, Da-Hai; Yao, Heng; Bai, Ge; Zhang, Yi-Han; Xiao, Bing-Guang

    2016-01-15

    Drought is one of the most severe forms of abiotic stresses that threaten the survival of plants, including crops. In turn, plants dramatically change their physiology to increase drought tolerance, including reconfiguration of proteomes. Here, we studied drought-induced proteomic changes in leaves of cultivated tobacco (Nicotiana tabacum), a solanaceous plant, using the isobaric tags for relative and absolute quantitation (iTRAQ)-based protein labeling technology. Of identified 5570 proteins totally, drought treatment increased and decreased abundance of 260 and 206 proteins, respectively, compared with control condition. Most of these differentially regulated proteins are involved in photosynthesis, metabolism, and stress and defense. Although abscisic acid (ABA) levels greatly increased in drought-treated tobacco leaves, abundance of detected ABA biosynthetic enzymes showed no obvious changes. In contrast, heat shock proteins (HSPs), thioredoxins, ascorbate-, glutathione-, and hydrogen peroxide (H2O2)-related proteins were up- or down-regulated in drought-treated tobacco leaves, suggesting that chaperones and redox signaling are important for tobacco tolerance to drought, and it is likely that redox-induced posttranslational modifications play an important role in modulating protein activity. This study not only provides a comprehensive dataset on overall protein changes in drought-treated tobacco leaves, but also shed light on the mechanism by which solanaceous plants adapt to drought stress. PMID:26692494

  11. Need to reform education in graduate schools of public health in Japan: Toward outcome-based education to cultivate competencies.

    Science.gov (United States)

    Inoue, Mariko; Yano, Eiji

    2016-01-01

    Japan, known for its good healthcare access via universal health insurance, leads the world in terms of life expectancy, and possesses a public health system that has improved health standards markedly in the 20th century. However, we currently face major challenges to maintain and promote people's health. Although these complicated problems pose numerous threats to public welfare, education of public health for health professionals still retains 20th-century standards. This also means that graduate education of public health in Japan is traditionally based on obtaining licensure as a medical professional, conducting research and writing papers, and on-the-job training. Since graduate school education is expected to produce competent public health leaders, Japan requires a reform toward a new education design that caters to the current societal needs. The current global trend in the education of health professionals leans toward outcome-based education to meet core competencies. Here, "competency" refers to a set of features or particular behavioral patterns possessed by highly qualified persons. In 2006, the World Health Organization (WHO) established a general health professional competency standard that includes both management and leadership competencies. Moreover, the Lancet Commission concluded that there was a need for transformative education based on a "health system approach." In brief, this means that our education should correspond to the needs of the health system to allow for the resolution of problems by educated professionals with satisfactory levels of competencies. In addition, as "change agents," these competent professionals are expected to promote societal change toward the realization of better public health. In Japan, the Central Education Council has produced several reports on professional graduate school reform since 2000. These reports indicate that graduate school curricula require reform to allow the health professionals to work

  12. Recommendations for the Regionalizing of Coffee Cultivation in Colombia: A Methodological Proposal Based on Agro-Climatic Indices

    OpenAIRE

    García L., Juan Carlos; Posada-Suárez, Húver; Läderach, Peter

    2014-01-01

    The Colombian National Federation of Coffee Growers (FNC) conducted an agro-ecological zoning study based on climate, soil, and terrain of the Colombian coffee-growing regions (CCGR) located in the tropics, between 1° and 11.5° N, in areas of complex topography. To support this study, a climate baseline was constructed at a spatial resolution of 5 km. Twenty-one bioclimatic indicators were drawn from this baseline data and from yield data for different coffee genotypes evaluated under conditi...

  13. A Property Restriction Based Knowledge Merging Method

    Science.gov (United States)

    Che, Haiyan; Chen, Wei; Feng, Tie; Zhang, Jiachen

    Merging new instance knowledge extracted from the Web according to certain domain ontology into the knowledge base (KB for short) is essential for the knowledge management and should be processed carefully, since this may introduce redundant or contradictory knowledge, and the quality of the knowledge in the KB, which is very important for a knowledge-based system to provide users high quality services, will suffer from such "bad" knowledge. Advocates a property restriction based knowledge merging method, it can identify the equivalent instances, redundant or contradictory knowledge according to the property restrictions defined in the domain ontology and can consolidate the knowledge about equivalent instances and discard the redundancy and conflict to keep the KB compact and consistent. This knowledge merging method has been used in a semantic-based search engine project: CRAB and the effect is satisfactory.

  14. Cloud Based Development Issues: A Methodical Analysis

    Directory of Open Access Journals (Sweden)

    Sukhpal Singh

    2012-11-01

    Full Text Available Cloud based development is a challenging task for various software engineering projects, especifically for those which demand extraordinary quality, reusability and security along with general architecture. In this paper we present a report on a methodical analysis of cloud based development problems published in major computer science and software engineering journals and conferences organized by various researchers. Research papers were collected from different scholarly databases using search engines within a particular period of time. A total of 89 research papers were analyzed in this methodical study and we categorized into four classes according to the problems addressed by them. The majority of the research papers focused on quality (24 papers associated with cloud based development and 16 papers focused on analysis and design. By considering the areas focused by existing authors and their gaps, untouched areas of cloud based development can be discovered for future research works.

  15. Method for a human based design process

    OpenAIRE

    Van Herck, Tine

    2013-01-01

    We are developing a method for a human based design process. This means a design method that enables to improve the social relevance of projects. It can be applied to projects of different scale : from interior architecture to landscape strategies. What should be the attitude of a designer in today's society where all certainties threaten to disappear? How to contribute something small to society in order to create a stronger tissue, a safety net, a solid found...

  16. Recommendation advertising method based on behavior retargeting

    Science.gov (United States)

    Zhao, Yao; YIN, Xin-Chun; CHEN, Zhi-Min

    2011-10-01

    Online advertising has become an important business in e-commerce. Ad recommended algorithms are the most critical part in recommendation systems. We propose a recommendation advertising method based on behavior retargeting which can avoid leakage click of advertising due to objective reasons and can observe the changes of the user's interest in time. Experiments show that our new method can have a significant effect and can be further to apply to online system.

  17. The Effect of Cultivated Wild Ginseng Extract on Preadipocyte Proliferation

    OpenAIRE

    Byoung-Woo Kim; Ki-Rok Kwon

    2007-01-01

    Objectives : The purpose of this study is to investigate the effects of cultivated wild ginseng extract on primary cultured preadipocyte and adipocytes. Methods : Diminish preadipocyte proliferation does primary role to reduce obesity. So, preadipocytes and adipocytes were performed on cell cultures with using Sprague-Dawley rats and treated with 0.01-1mg/㎖ cultivated wild ginseng extract. Result : At all concentrations, cultivated wild ginseng extract wasn't show the suppress prolifera...

  18. Plants cultivation in controlled containments

    International Nuclear Information System (INIS)

    The plants cultivation in controlled containments permits to the - Departement d'Ecophysiologie Vegetale et de Microbiologie (DVEM) - of the CEA to lead several topics of research. The works of DVEM which are based on the molecular labelling, technique adapted to plants, contribute to understand the plant - soil relationships and the plant growth process. In addition, the staff of DVEM study the impact of pollutant heavy metals, existing in the soil, on plants and the plant stress induced by oxygen, light, ionizing radiations,... and defence mechanisms of plants (F. M.)

  19. How to Design and Present Texts to Cultivate Balanced Regional Images in Geography Education

    Science.gov (United States)

    Lee, Dong-Min; Ryu, Jaemyong

    2013-01-01

    This article examines possibilities associated with the cultivation of balanced regional images via the use of simple methods. Two experiments based on the primacy effect and the painting picture rule, or visual depiction of regions, were conducted. The results show significant differences in the formation of regional images. More specifically,…

  20. Investigation of the microbial community in the Odisha hot spring cluster based on the cultivation independent approach

    Science.gov (United States)

    Singh, Archana; Subudhi, Enketeswara; Sahoo, Rajesh Kumar; Gaur, Mahendra

    2016-01-01

    Deulajhari hot spring is located in the Angul district of Odisha. The significance of this hot spring is the presence of the hot spring cluster adjacent to the cold spring which attracts the attention of microbiologists to understand the role of physio-chemical factors of these springs on bacterial community structure. Next-generation sequencing technology helps us to depict the pioneering microflora of any ecological niche based on metagenomic approach. Our study represents the first Illumina based metagenomic study of Deulajhari hot spring DH1, and DH2 of the cluster with temperature 65 °C to 55 °C respectively establishing a difference of 10 °C. Comprehensive study of microbiota of these two hot springs was done using the metagenomic sequencing of 16S rRNA of V3‐V4 region extracting metagenomic DNA from the two hot spring sediments. Sequencing community DNA reported about 28 phyla in spring DH1 of which the majority were Chloroflexi (22.98%), Proteobacteria (15.51%), Acidobacteria (14.51%), Chlorobi (9.52%), Nitrospirae (8.54%), and Armatimonadetes (7.07%), at the existing physiochemical conditions like; temperature 65 °C, pH 8.06, electro conductivity 0.020 dSm− 1, and total organic carbon (TOC) 3.76%. About 40 phyla were detected in cluster DH2 at the existing physiochemical parameters like temperature 55 °C, pH 8.10, electro conductivity 0.019 dSm− 1, and total organic carbon (TOC) 0.58% predominated with Chloroflexi (41.98%), Proteobacteria (10.74%), Nitrospirae (10.01%), Chlorobi (8.73%), Acidobacteria (6.73%) and Planctomycetes (3.73%). Approximately 68 class, 107 order, 171 genus and 184 species were reported in cluster DH1 but 102 class, 180 order, 375 genus and 411 species in cluster DH2. The comparative metagenomics study of the Deulajhari hot spring clusters DH1, and DH2 depicts the differential profile of the microbiota. Metagenome sequences of these two hot spring clusters are deposited to the SRA database and are available in

  1. Rough set-based feature selection method

    Institute of Scientific and Technical Information of China (English)

    ZHAN Yanmei; ZENG Xiangyang; SUN Jincai

    2005-01-01

    A new feature selection method is proposed based on the discern matrix in rough set in this paper. The main idea of this method is that the most effective feature, if used for classification, can distinguish the most number of samples belonging to different classes. Experiments are performed using this method to select relevant features for artificial datasets and real-world datasets. Results show that the selection method proposed can correctly select all the relevant features of artificial datasets and drastically reduce the number of features at the same time. In addition, when this method is used for the selection of classification features of real-world underwater targets,the number of classification features after selection drops to 20% of the original feature set, and the classification accuracy increases about 6% using dataset after feature selection.

  2. Virtual Situational Teaching Method in the Application of Professional English Talent Cultivation Research%虚拟情境教学法在专业英语人才培养中的应用研究

    Institute of Scientific and Technical Information of China (English)

    王梅亮; 叶秋群

    2014-01-01

    为了适应新的经济时代对应用型英语专业人才的需求,迫切需要改革目前专业英语的教学模式。该文首先根据建构主义教学理论,探讨虚拟情境教学法在专业英语教学中应用的优越性;然后研究如何在专业英语课堂教学中利用虚拟情境教学系统,为学生营造出逼真的语言学习和运用环境,通过情景体验进行教学和操练来提高学英语的学习效果,为培养应用型英语专业人才探索出一种全新而且有效的教学模式和教学手段。%In order to adapt to the new economic era demand for applied English professional talents, the current professional English teaching mode need to be reformed urgently.The paper based on the constructivism teaching theory, discusses the applica-tion of virtual situational teaching method in the professional English teaching superiority. And then it studied how to use virtual situation teaching in English classroom teaching system, in order to create a vivid language learning and using environment for students. Through the scene experience and practice of teaching to improve the learning effect of learning English, we can culti-vate applied English professional talents and explore a new effective teaching mode and teaching means.

  3. Evidence for a Common Origin of Blacksmiths and Cultivators in the Ethiopian Ari within the Last 4500 Years: Lessons for Clustering-Based Inference.

    Science.gov (United States)

    van Dorp, Lucy; Balding, David; Myers, Simon; Pagani, Luca; Tyler-Smith, Chris; Bekele, Endashaw; Tarekegn, Ayele; Thomas, Mark G; Bradman, Neil; Hellenthal, Garrett

    2015-08-01

    The Ari peoples of Ethiopia are comprised of different occupational groups that can be distinguished genetically, with Ari Cultivators and the socially marginalised Ari Blacksmiths recently shown to have a similar level of genetic differentiation between them (FST ≈ 0.023 - 0.04) as that observed among multiple ethnic groups sampled throughout Ethiopia. Anthropologists have proposed two competing theories to explain the origins of the Ari Blacksmiths as (i) remnants of a population that inhabited Ethiopia prior to the arrival of agriculturists (e.g. Cultivators), or (ii) relatively recently related to the Cultivators but presently marginalized in the community due to their trade. Two recent studies by different groups analysed genome-wide DNA from samples of Ari Blacksmiths and Cultivators and suggested that genetic patterns between the two groups were more consistent with model (i) and subsequent assimilation of the indigenous peoples into the expanding agriculturalist community. We analysed the same samples using approaches designed to attenuate signals of genetic differentiation that are attributable to allelic drift within a population. By doing so, we provide evidence that the genetic differences between Ari Blacksmiths and Cultivators can be entirely explained by bottleneck effects consistent with hypothesis (ii). This finding serves as both a cautionary tale about interpreting results from unsupervised clustering algorithms, and suggests that social constructions are contributing directly to genetic differentiation over a relatively short time period among previously genetically similar groups. PMID:26291793

  4. Evidence for a Common Origin of Blacksmiths and Cultivators in the Ethiopian Ari within the Last 4500 Years: Lessons for Clustering-Based Inference.

    Directory of Open Access Journals (Sweden)

    Lucy van Dorp

    2015-08-01

    Full Text Available The Ari peoples of Ethiopia are comprised of different occupational groups that can be distinguished genetically, with Ari Cultivators and the socially marginalised Ari Blacksmiths recently shown to have a similar level of genetic differentiation between them (FST ≈ 0.023 - 0.04 as that observed among multiple ethnic groups sampled throughout Ethiopia. Anthropologists have proposed two competing theories to explain the origins of the Ari Blacksmiths as (i remnants of a population that inhabited Ethiopia prior to the arrival of agriculturists (e.g. Cultivators, or (ii relatively recently related to the Cultivators but presently marginalized in the community due to their trade. Two recent studies by different groups analysed genome-wide DNA from samples of Ari Blacksmiths and Cultivators and suggested that genetic patterns between the two groups were more consistent with model (i and subsequent assimilation of the indigenous peoples into the expanding agriculturalist community. We analysed the same samples using approaches designed to attenuate signals of genetic differentiation that are attributable to allelic drift within a population. By doing so, we provide evidence that the genetic differences between Ari Blacksmiths and Cultivators can be entirely explained by bottleneck effects consistent with hypothesis (ii. This finding serves as both a cautionary tale about interpreting results from unsupervised clustering algorithms, and suggests that social constructions are contributing directly to genetic differentiation over a relatively short time period among previously genetically similar groups.

  5. Cost effective and economic method for cultivation of Chlorella pyrenoidosa for the simultaneous treatment of biogas digester wastewater and biogas production

    Directory of Open Access Journals (Sweden)

    Rohit Sharma

    2015-02-01

    Full Text Available Microalgae have recently received a lot of attention as a new biomass source for the production of bio fuels and for the treatment of waste water. In this work, Chlorella pyrenoidosa was cultivated in biogas digester wastewater. The growth kinetics of the algae as well as the bio-remediation effect on the waste water was studied. The Chlorella pyrenoidosa can utilize the nitrogen content present in biogas digester wastewater as a substrate for its growth. The growth of microalgae was found to follow the Monod growth model satisfactorily. Under the optimal condition in biogas waste water medium of microalgae, a maximum biomass of 1.5 gm/l was obtained in fifteen days. The net specific growth rate of microalgae Chlorella pyrenoidosa was found to be 0.1 D −1. The growing algae also removed 76 % of nitrate nitrogen (NO3-N from the biogas wastewater. Treated Biogas waste water can be further used for the anaerobic digestion of algal biomass for the production of biogas. This suggests that the cultivation of Chlorella pyrenoidosa in biogas wastewater would be efficient, saving water as well as producing digestible biomass. Thus, on one hand the biogas waste water is being treated and on the other, the alga is showing substantial growth. The same algal species can then be used to produce biogas or other corresponding bio fuel.

  6. 创新高校学生干部队伍培养之路%Innovation of the Cultivation Methods for College Student Cadre Group

    Institute of Scientific and Technical Information of China (English)

    方君亮; 宣玉梅; 成晓

    2011-01-01

    高校学生干部是大学校园中的一个特殊群体,也是高校学生工作的一个重要方面。如何打造一支高素质的学生干部队伍,对于做好高校的思想政治工作和学生工作都具有重要意义。本文着重研究如何培养学生干部自身素质、如何培养学生干部团队合作意识和如何在活动中提升学生干部队伍综合素质。%Student cadre is a special group on college campus and an important aspect of college student affairs.How to construct a high quality student cadre group is of great significance to implement college ideological and political work and student affairs.This paper mainly studies on how to cultivate the quality of student cadres,how to cultivate the teamwork awareness of student cadre group and how to improve the comprehensive quality of student cadre group.

  7. Bayesian individualization via sampling-based methods.

    Science.gov (United States)

    Wakefield, J

    1996-02-01

    We consider the situation where we wish to adjust the dosage regimen of a patient based on (in general) sparse concentration measurements taken on-line. A Bayesian decision theory approach is taken which requires the specification of an appropriate prior distribution and loss function. A simple method for obtaining samples from the posterior distribution of the pharmacokinetic parameters of the patient is described. In general, these samples are used to obtain a Monte Carlo estimate of the expected loss which is then minimized with respect to the dosage regimen. Some special cases which yield analytic solutions are described. When the prior distribution is based on a population analysis then a method of accounting for the uncertainty in the population parameters is described. Two simulation studies showing how the methods work in practice are presented. PMID:8827585

  8. Nataf transformation based point estimate method

    Institute of Scientific and Technical Information of China (English)

    LI HongShuang; L(U) ZhenZhou; YUAN XiuKai

    2008-01-01

    Structural probabilistic analysis quantifies the effect of input random variables, such as material proper-ties, geometrical parameters and loading conditions, on the structural responses. The point estimate method (PEM) is a direct and easy-used way to perform the structural probabilistic analysis in practice. In this paper, a novel and efficient point estimate method is proposed for computing the first four statistical moments of structural response which is a function of input random variables. The method adopts Nataf transformation to replace Rosenblatt transformation in conventional point estimate method. Because of the nature of engineering problems and limited statistical data, the joint probability density function (PDF) of all input random variables is hard to acquire, but it must be known in Rosenblatt transformation. A more common case is that the marginal PDF of each random variable and the correlation matrix are available, which just satisfy the service condition of Nataf transformation. Hence the Nataf transformation based point estimate method is particularly suitable for engineering applications. The comparison be-tween the proposed method and the conventional point estimate method shows that (1) they are equiva-lent when all random variables are mutually independent; (2) if the marginal PDFs and the correlation matrix are known, the conventional PEM cannot be applicable, but the proposed method can give a ra-tional approximation. Finally, the procedure is demonstrated in detail through a simple illustration.

  9. 基于Unity3D的甘蔗种植虚拟教育培训系统设计与实现%Design and Realization of Virtual Education Training System for Sugarcane Cultivation Based on Unity3D

    Institute of Scientific and Technical Information of China (English)

    吴升; 郭新宇; 贺谊; 温维亮; 王虓

    2014-01-01

    我国是最大的甘蔗种植国之一,甘蔗种植教育培训课程在各大农林职业院校广泛开展,针对传统的甘蔗种植教育培训试验开展周期长、成本高、授课方式枯燥、参与实践机会少等不足。为此开展甘蔗生长模型建模,农事仿真实时可视化等虚拟仿真交互关键技术进行研究;以“看动画、学知识、玩游戏、长技能”的虚拟互动培训理念,提出基于时序驱动的甘蔗虚拟种植教育培训系统设计方法;以Unity3D为开发平台,实现甘蔗种植生长周期虚拟展示以及互动体验功能。系统以虚拟化改造传统教学,为现代农业职业技术教育提供新的教学思路,从而为全面提升学员技术技能水平做出贡献。%China is one of the largest sugarcane cultivation countries. Education training courses for sugarcane cultivation is conducted in vocational colleges of agriculture and forestry universities. On account of the shortcomings of the conventional sugarcane education and training, such as long cycles, high costs, boring teaching methods, few practice opportunities, etc., we carried out studies on key technology of sugarcane interactive virtual simulation, sugarcane growth modeling, sugarcane planting real⁃time visualization simulation, etc. Method for designing sugarcane virtual education training system based on timing driven was provided, taking the concept of “watching animation, learning knowledge, playing games, and mastering skill” throughout the whole teaching process. Thus, taking Unity3D as a development platform, we realized the virtual display of sugarcane growth cycle and interactive experience function. This system took virtualization to reform the traditional education, and provided new teaching ideas for modern vocational and technical education in agriculture, thus had comprehensive upgrading of the students’ technical skill level.

  10. ECONOMICS OF CULTIVATION IN ANDHRA PRADESH

    Directory of Open Access Journals (Sweden)

    Balakrishna Ankalam

    2014-10-01

    Full Text Available Farming as a business is greatly influenced by the level and structure of cost of cultivation. Before green revolution farmers were practicing traditional farming methods in which they use home supplied inputs with conventional practices. The risk and uncertainty associated with cultivation does not induce the farmers to invest more on farm business. This in turn leads to low level of input use. In cultivation farmers are constrained to take up more remunerative crop enterprises. People depending on this sector are characterized by low income and higher poverty. To acquire comprehensive understanding of the problems prevailing in agriculture, a Multistage Random Sampling Method is employed. In the first step Andhra Pradesh is divided into three regions, namely Coastal Andhra, Telangana and Rayalaseema regions. The households are divided into five farming categories.

  11. Application of Reduced Corn Cultivation Technology in Agro-Ecosystem of Cazin Municipality

    Directory of Open Access Journals (Sweden)

    Mirsad Veladžić

    2011-03-01

    Full Text Available Intensive corn cultivation is predominant in current agriculture of the Una-Sana Canton. One of the corn cultivation methods in agro-ecosystem is reduced cultivation. The paper presents the experiment of “Osmak žuti” (eight-row yellow corn cultivation on two control sites with application of reduced and intensive cultivation in Cazin municipality. The objectives of this research were to examine the possibility of application of reduced corn cultivation; analyze statistical variation elements (length, circumference and weight of corn cob; and determine cost effectiveness of reduced relative to intensive production. The results indicate extremely high statistically significant difference (p<0.001 for all parameters in both cultivation methods. The cost effectiveness of reduced relative to intensive cultivation of Osmak žuti corn is higher by 36%. The experiment encourages introduction of reduced corn cultivation practice.

  12. Studies on Edible Fungi Cultivation Based on Oilseed Rape Straw%油菜秸秆栽培食用菌研究综述

    Institute of Scientific and Technical Information of China (English)

    宋海燕; 胡殿明

    2015-01-01

    Edible fungi cultivation is a sufficient way to reuse the massive oilseed rape straw without any environment pollution. In this paper, the literatures reported on edible fungi cultivation using the substrate of oilseed rape straw in China were briefly reviewed mainly on the aspects of medium formulations and fungal species. According to our statistic, the literatures that reported on edible fungi cultivation using oilseed rape straw reported in China were manly focused on the cultivation of Pleurotus spp. and Flammulina velutipes, while only a few literatures reported on the cultivation of Hypsizygus marmoreus, Volvariella volvacea and Lentinus edodes. Most reports just briefly reported the medium formulations for edible fungi cultivation, without other details such as pretreatment of raw materials, records of hyphae growth, temperature, humidity, light, the concentration of CO2, etc. In this paper, the medium formulations using oilseed rape straw reported by literatures were all listed in 3 tables, they included 29 formulations for the cultivation of Pleurotus spp., 13 formulations for the cultivation of Flammulina velutipes and 6 formulations for the cultivation of other edible fungi. The biological transformation efficiency of each formulation using oilseed rape straw was discussed. The biological transformation efficiency of most formulations mainly composed by oilseed rape straw was commonly lower than the biological transformation efficiency of traditional formulations, however, most of the former formulations presented higher economic input­output ratio than the latter, as oilseed rape straw was normally cheaper than the traditional substrates such as cotton seed hulls, sawdust etc. Furthermore, mushroom hyphae normally grew faster on oilseed rape straw substrates than traditional substrates, which could significantly shorten the cultivation period of edible fungi and subsequently reduce the cost of the cultivation of edible fungi. Therefore, oilseed rape

  13. Building a field- and model-based climatology of local water and energy cycles in the cultivated Sahel - annual budgets and seasonality

    Science.gov (United States)

    Velluet, C.; Demarty, J.; Cappelaere, B.; Braud, I.; Issoufou, H. B.-A.; Boulain, N.; Ramier, D.; Mainassara, I.; Charvet, G.; Boucher, M.; Chazarin, J.-P.; Oï, M.; Yahou, H.; Maidaji, B.; Arpin-Pont, F.; Benarrosh, N.; Mahamane, A.; Nazoumou, Y.; Favreau, G.; Seghieri, J.

    2014-05-01

    In the African Sahel, energy and water cycling at the land surface is pivotal for regional climate, water resources and land productivity, yet it is still extremely poorly documented. As a step towards a comprehensive climatological description of surface fluxes in this area, this study provides estimates of average annual budgets and seasonal cycles for two main land use types of the cultivated Sahelian belt, rainfed millet crop and fallow bush. These estimates build on the combination of a 7 year field dataset from two typical plots in southwestern Niger with detailed physically-based soil-plant-atmosphere modelling, yielding a continuous, comprehensive set of water and energy flux and storage variables over the 7 year period. In this study case in particular, blending field data with mechanistic modelling is considered as making best use of available data and knowledge for such purpose. It extends observations by reconstructing missing data and extrapolating to unobserved variables or periods. Furthermore, model constraining with observations compromises between extraction of observational information content and integration of process understanding, hence accounting for data imprecision and departure from physical laws. Climatological averages of all water and energy variables, with associated sampling uncertainty, are derived at annual to subseasonal scales from the 7 year series produced. Similarities and differences in the two ecosystems behaviors are highlighted. Mean annual evapotranspiration is found to represent ~82-85% of rainfall for both systems, but with different soil evaporation/plant transpiration partitioning and different seasonal distribution. The remainder consists entirely of runoff for the fallow, whereas drainage and runoff stand in a 40-60% proportion for the millet field. These results should provide a robust reference for the surface energy- and water-related studies needed in this region. The model developed in this context has the

  14. Molecular Characterization of Cultivated Bromeliad Accessions with Inter-Simple Sequence Repeat (ISSR Markers

    Directory of Open Access Journals (Sweden)

    Yongming Yu

    2012-05-01

    Full Text Available Bromeliads are of great economic importance in flower production; however little information is available with respect to genetic characterization of cultivated bromeliads thus far. In the present study, a selection of cultivated bromeliads was characterized via inter-simple sequence repeat (ISSR markers with an emphasis on genetic diversity and population structure. Twelve ISSR primers produced 342 bands, of which 287 (~84% were polymorphic, with polymorphic bands per primer ranging from 17 to 34. The Jaccard’s similarity ranged from 0.08 to 0.89 and averaged ~0.30 for the investigated bromeliads. The Bayesian-based approach, together with the un-weighted paired group method with arithmetic average (UPGMA-based clustering and the principal coordinate analysis (PCoA, distinctly grouped the bromeliads from Neoregelia, Guzmania, and Vriesea into three separately clusters, well corresponding with their botanical classifications; whereas the bromeliads of Aechmea other than the recently selected hybrids were not well assigned to a cluster. Additionally, ISSR marker was proven efficient for the identification of hybrids and bud sports of cultivated bromeliads. The findings achieved herein will further our knowledge about the genetic variability within cultivated bromeliads and therefore facilitate breeding for new varieties of cultivated bromeliads in future as well.

  15. Molecular characterization of cultivated bromeliad accessions with Inter-Simple Sequence Repeat (ISSR) Markers.

    Science.gov (United States)

    Zhang, Fei; Ge, Yaying; Wang, Weiyong; Yu, Xinying; Shen, Xiaolan; Liu, Jianxin; Liu, Xiaojing; Tian, Danqing; Shen, Fuquan; Yu, Yongming

    2012-01-01

    Bromeliads are of great economic importance in flower production; however little information is available with respect to genetic characterization of cultivated bromeliads thus far. In the present study, a selection of cultivated bromeliads was characterized via inter-simple sequence repeat (ISSR) markers with an emphasis on genetic diversity and population structure. Twelve ISSR primers produced 342 bands, of which 287 (~84%) were polymorphic, with polymorphic bands per primer ranging from 17 to 34. The Jaccard's similarity ranged from 0.08 to 0.89 and averaged ~0.30 for the investigated bromeliads. The Bayesian-based approach, together with the un-weighted paired group method with arithmetic average (UPGMA)-based clustering and the principal coordinate analysis (PCoA), distinctly grouped the bromeliads from Neoregelia, Guzmania, and Vriesea into three separately clusters, well corresponding with their botanical classifications; whereas the bromeliads of Aechmea other than the recently selected hybrids were not well assigned to a cluster. Additionally, ISSR marker was proven efficient for the identification of hybrids and bud sports of cultivated bromeliads. The findings achieved herein will further our knowledge about the genetic variability within cultivated bromeliads and therefore facilitate breeding for new varieties of cultivated bromeliads in future as well. PMID:22754348

  16. An image mosaic method based on corner

    Science.gov (United States)

    Jiang, Zetao; Nie, Heting

    2015-08-01

    In view of the shortcomings of the traditional image mosaic, this paper describes a new algorithm for image mosaic based on the Harris corner. Firstly, Harris operator combining the constructed low-pass smoothing filter based on splines function and circular window search is applied to detect the image corner, which allows us to have better localisation performance and effectively avoid the phenomenon of cluster. Secondly, the correlation feature registration is used to find registration pair, remove the false registration using random sampling consensus. Finally use the method of weighted trigonometric combined with interpolation function for image fusion. The experiments show that this method can effectively remove the splicing ghosting and improve the accuracy of image mosaic.

  17. Reinforcement Learning Based on Active Learning Method

    CERN Document Server

    Sagha, Hesam; Khasteh, Hosein; Kiaei, Ali Akbar

    2010-01-01

    In this paper, a new reinforcement learning approach is proposed which is based on a powerful concept named Active Learning Method (ALM) in modeling. ALM expresses any multi-input-single-output system as a fuzzy combination of some single-input-singleoutput systems. The proposed method is an actor-critic system similar to Generalized Approximate Reasoning based Intelligent Control (GARIC) structure to adapt the ALM by delayed reinforcement signals. Our system uses Temporal Difference (TD) learning to model the behavior of useful actions of a control system. The goodness of an action is modeled on Reward- Penalty-Plane. IDS planes will be updated according to this plane. It is shown that the system can learn with a predefined fuzzy system or without it (through random actions).

  18. AN SVAD ALGORITHM BASED ON FNNKD METHOD

    Institute of Scientific and Technical Information of China (English)

    Chen Dong; Zhang Yan; Kuang Jingming

    2002-01-01

    The capacity of mobile communication system is improved by using Voice Activity Detection (VAD) technology. In this letter, a novel VAD algorithm, SVAD algorithm based on Fuzzy Neural Network Knowledge Discovery (FNNKD) method is proposed. The performance of SVAD algorithm is discussed and compared with traditional algorithm recommended by ITU G.729B in different situations. The simulation results show that the SVAD algorithm performs better.

  19. 不同水直播方式水稻植株抗倒特性研究%Lodging Resistance of Rice under the Different Pattern of Water Direct-seeding Cultivation Method

    Institute of Scientific and Technical Information of China (English)

    许轲; 唐磊; 郭保卫; 张洪程; 霍中洋; 戴其根; 魏海燕

    2014-01-01

    cultivation,by meas-uring the morphological and physical traits of rice basal stems. Furthermore, the correlation analysis between the lodging index,breaking resistance and main culm physical characteristics were studied. The results indicated that the culm lodging resistance of rice planted with different planting methods was significantly different. In field,the lodging rate of hole seeding rice was lower than that of seeding rice in drill. Compared with seeding rice in drill,the breaking resistance of the second basal internode and third basal internode in hole seeding rice was higher,while the lodging index was lower. Plant height and gravity center height in hole seeding rice was longer with the ratio of grav-ity center height to plant height shorter. In hole seeding rice,length of the first and second basal internode was sig-nificantly shorter,culm inside diameter of three internodes from base was longer, and beside the first basal inter-node,culm outside diameter and culm wall thickness of the second basal internode and third basal internode were longer,and dry weight of unit internode of the first basal internode and the second basal internode were higher. Those culm physical traits of different type varieties were significantly different. The breaking resistance of the 1st, 2nd,3rd basal internode and plant height,gravity center height,culm diameter,culm wall thickness,dry weight of culm,dry weight of unit internode and bending moment had significant or very significant positive correlation,while internodes length showed a significant or very significant negative correlation. The optimal combination of some main culm physical traits of rice was different under different planting methods. The direct reasons for lodging resistance increased in hole seeding rice were short and thick basal internodes,large wall thickness and good culm plumpness status.

  20. Black truffle cultivation: a global reality

    Directory of Open Access Journals (Sweden)

    Santiago Reyna

    2014-08-01

    Full Text Available Aim of study: In recent decades the cultivation of the black truffle Tuber melanosporum has expanded across all the Mediterranean-climate regions, but also to other regions outside the European standard for the species. We aim to describe the current extent of T. melanosporum cultivation.Area of study: Tuber melanosporum plantations in Europe, the Mediterranean basin, Australia, New Zealand, China, America and South Africa.Material and Methods: The socioeconomic impact of T. melanosporum cultivation, the way in which the current situation has been achieved and the knowledge needed for its progress are reviewed.Research highlights: T. melanosporum has been successfully cultivated in several countries outside its natural area, but many practices are still empirical and thus yields cannot be guaranteed. The recent advances in molecular techniques and genome science are helping to overcome some of the difficulties traditionally constraining truffle research. The role of truffles as a transitional element between agricultural and forestry activities makes its cultivation a paradigm of sustainable rural development.Keywords: Tuber melanosporum; Europe; Australia; New Zealand; Chile; USA.

  1. An Evaluation of the Externality of Cultivated Land Based on CVM:A Case Study of Neihuang County, Henan Province%基于CVM耕地资源利用的外部性评估——以河南省内黄县为例

    Institute of Scientific and Technical Information of China (English)

    陈艳蕊; 赵凯; 王志晓; 薛欧

    2011-01-01

    improve farmland landscape. This helps enhance farmer's awareness to protect cultivated land so as to improve the overall efficiency of land utilization. Based on questionnaire surveys about Neihuang County in Henan Province, we performed Pearson correlation tests about the socio-economic characteristics, and subsequently multiple linear regression analysis on the dependent variables of LnWTP. In addition, we assessed the Externality of the Cultivated Land with Contingent Valuation Method (CVM) and analyzed its influential factors. Results show that: 1) the relationship between annual income of urban households and their willingness to pay is statistically significantly positive; 2) the relationship between the age of rural residents and the corresponding area of contracted cultivated land and their willingness to pay is statistically significantly negative; the relationship among gender, education level, household annual income, agricultural income, and their willingness to pay are generally positive; and 3) based on the median values of 75.5 yuan as the urban residents' willingness to pay, and 15.5 yuan as the willingness to pay of the rural residents, the externality of the cultivated land resources in Neihuang County, Henan Province, was estimated to be 11 649.5 yuan/hm2 (776.6 yuan/mu). Thus, more attention should be paid to the value of the externality of the cultivated land resources in estimating its total value, expropriation price, transaction price, and to policy making with respect to protection of cultivated land resources.

  2. Cultivation of EFL Learners’Intercultural Competence

    Institute of Scientific and Technical Information of China (English)

    夏晨

    2012-01-01

      Nowadays, educators from home and abroad believe that learning a foreign language means learning a great deal of the foreign civilization and culture. And the utmost goal of language teaching is to cultivate EFL learners’communicative competence, but not merely the linguistic knowledge. Thus, teaching a foreign language is more than teaching new words, expressions and grammar rules, but should also incorporate the target culture elements into language knowledge. In order to improve EFL learners’English proficiency, teachers must make efforts to cultivate EFL learners’intercultural competence and promote their sensibility and adaptability to the differences between Chinese culture and the Western culture. Only by doing so, can EFL learners communicate with native speakers appropriately and smoothly. This pa-per proposes some effective teaching techniques and methods that can be applied in English classroom to cultivate Chinese EFL learners’in-tercultural competence.

  3. Lagrangian based methods for coherent structure detection

    International Nuclear Information System (INIS)

    There has been a proliferation in the development of Lagrangian analytical methods for detecting coherent structures in fluid flow transport, yielding a variety of qualitatively different approaches. We present a review of four approaches and demonstrate the utility of these methods via their application to the same sample analytic model, the canonical double-gyre flow, highlighting the pros and cons of each approach. Two of the methods, the geometric and probabilistic approaches, are well established and require velocity field data over the time interval of interest to identify particularly important material lines and surfaces, and influential regions, respectively. The other two approaches, implementing tools from cluster and braid theory, seek coherent structures based on limited trajectory data, attempting to partition the flow transport into distinct regions. All four of these approaches share the common trait that they are objective methods, meaning that their results do not depend on the frame of reference used. For each method, we also present a number of example applications ranging from blood flow and chemical reactions to ocean and atmospheric flows

  4. Lagrangian based methods for coherent structure detection

    Energy Technology Data Exchange (ETDEWEB)

    Allshouse, Michael R., E-mail: mallshouse@chaos.utexas.edu [Center for Nonlinear Dynamics and Department of Physics, University of Texas at Austin, Austin, Texas 78712 (United States); Peacock, Thomas, E-mail: tomp@mit.edu [Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 (United States)

    2015-09-15

    There has been a proliferation in the development of Lagrangian analytical methods for detecting coherent structures in fluid flow transport, yielding a variety of qualitatively different approaches. We present a review of four approaches and demonstrate the utility of these methods via their application to the same sample analytic model, the canonical double-gyre flow, highlighting the pros and cons of each approach. Two of the methods, the geometric and probabilistic approaches, are well established and require velocity field data over the time interval of interest to identify particularly important material lines and surfaces, and influential regions, respectively. The other two approaches, implementing tools from cluster and braid theory, seek coherent structures based on limited trajectory data, attempting to partition the flow transport into distinct regions. All four of these approaches share the common trait that they are objective methods, meaning that their results do not depend on the frame of reference used. For each method, we also present a number of example applications ranging from blood flow and chemical reactions to ocean and atmospheric flows.

  5. The Exploration and Practice of the Cultivating Models for Competition and Innovation Based Marketing Professionals%基于技能竞赛创新高职营销人才培养模式的探索与实践

    Institute of Scientific and Technical Information of China (English)

    曹成喜

    2012-01-01

    经济的转型急需与区域经济发展相适应的高职营销人才,文章以全国营销大赛为切入口,对创新高职营销人才培养的模式进行实践与探索,提出基于营销大赛创新高职营销专业人才培养模式的八个突破。%The restructuring of the economy entails an urgent need for professional marketing talents who can adapt to regional e- conomic developments. This thesis explores the cultivation models of innovative marketing professionals from the angle of the na- tional marketing competition and proposes several breakthroughs in the cultivating models based on the marketing competition.

  6. Phenylhydrazines in the cultivated mushroom (Agaricus bisporus)

    DEFF Research Database (Denmark)

    Andersson, H. C.; Gry, Jørn

    2004-01-01

    In 1991, the Nordic Working Group on Food Toxicology and Risk Evaluation (NNT) reviewed the available data on phenylhydrazines naturally occurring in the cultivated mushroom. It was concluded that the mushroom may contain about 500 mg of the hydrazine derivatives per kg fresh weight. The hydrazine...... derivatives as well as extracts of the cultivated mushroom were mutagenic to a variable degree in most of the reported short-term tests. The raw mushroom and several of the hydrazines induced tumours when administered to Swiss mice as reported by American scientists. However, reservations were expressed...... as to the design of the studies. Based on this review, and due to the concern expressed, a Nordic project (coordinated by Jørn Gry, Danish Veterinary and Food Administration) was initiated dealing with toxicological and chemical studies on the cultivated mushroom and its phenylhydrazine derivatives in order...

  7. Phase space bases for response matrix methods

    International Nuclear Information System (INIS)

    New basis functions in space, angle, and energy are developed for use in the eigenvalue response matrix method (ERMM). ERMM solves reactor eigenvalue problems by spatially decomposing a model and linking the resulting independent nodes through approximate boundary conditions. The conditions are defined via a set of nodal transport calculations for which the forcing function is a tensor product of basis functions in all phase space variables on one nodal surface (with vacuum elsewhere). Traditional implementations of ERMM have used the standard bases of mathematical physics, including Legendre polynomials and their discrete analogs. Alternatively, new bases that incorporate some physics a priori are shown to yield satisfactory results with far lower expansion orders than is possible with more traditional bases. In particular, a very simple spatial basis incorporating pin-dependent spatial variation reduces partial current errors by nearly an order of magnitude over a standard basis. In angle, conservative bases with appropriate angular quadratures outperform simpler bases. In energy, incorporating representative spectral information from infinite medium calculations results in a basis that can achieve sub-1% pin fission rate errors with as low as a 5th order expansion in energy for a 44-group problem. The results indicate that highly-accurate, low-order response matrix solutions should be feasible for reactor physics analysis. (author)

  8. Chapter 11. Community analysis-based methods

    Energy Technology Data Exchange (ETDEWEB)

    Cao, Y.; Wu, C.H.; Andersen, G.L.; Holden, P.A.

    2010-05-01

    Microbial communities are each a composite of populations whose presence and relative abundance in water or other environmental samples are a direct manifestation of environmental conditions, including the introduction of microbe-rich fecal material and factors promoting persistence of the microbes therein. As shown by culture-independent methods, different animal-host fecal microbial communities appear distinctive, suggesting that their community profiles can be used to differentiate fecal samples and to potentially reveal the presence of host fecal material in environmental waters. Cross-comparisons of microbial communities from different hosts also reveal relative abundances of genetic groups that can be used to distinguish sources. In increasing order of their information richness, several community analysis methods hold promise for MST applications: phospholipid fatty acid (PLFA) analysis, denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (TRFLP), cloning/sequencing, and PhyloChip. Specific case studies involving TRFLP and PhyloChip approaches demonstrate the ability of community-based analyses of contaminated waters to confirm a diagnosis of water quality based on host-specific marker(s). The success of community-based MST for comprehensively confirming fecal sources relies extensively upon using appropriate multivariate statistical approaches. While community-based MST is still under evaluation and development as a primary diagnostic tool, results presented herein demonstrate its promise. Coupled with its inherently comprehensive ability to capture an unprecedented amount of microbiological data that is relevant to water quality, the tools for microbial community analysis are increasingly accessible, and community-based approaches have unparalleled potential for translation into rapid, perhaps real-time, monitoring platforms.

  9. High-effective cultivation of Halobacterium salinarum providing with bacteriorhodopsin production under controlled stress.

    Science.gov (United States)

    Kalenov, Sergei V; Baurina, Marina M; Skladnev, Dmitry A; Kuznetsov, Alexander Ye

    2016-09-10

    Submerged growth of Halobacterium salinarum and therefore synthesis of bacteriorhodopsin (BR) and carotenoids depend greatly on products of both chemical and/or photochemical oxidation of medium components and cellular metabolism which act as inhibitors. Some cultivation variants which allowed eliminating an adverse effect of inhibitors on biomass accumulation and BR synthesis are reviewed. The application of activated charcoal or ion exchange resin as adsorbents at preparing inoculums and the main cultivation stages was shown to allow controlling, namely lowering overstress of the halobacterial cells by metabolites. The halobacterial biomass containing BR up to 1,750mgL(-1) and the minimum amount of carotinoids that would BR greatly facilitate isolation was accumulated up to 45gL(-1) during eight-day cultivation with cell recycling through adsorbent suspension in a fed-batch mode. To control BR biosynthesis the express method of BR quantification based on colour shades of cell suspension was developed. PMID:27449487

  10. Effect of cultivation measures on economic benefit of Larix olgesis pulp forest

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    According to the cultivating practice of Larix olgensis pulp plantation, IRR (Internal revenue rate) and NPV (Net present value) were taken as two economic indices to study the effect of cultivation measurements on economic benefit of Larix olgensis pulp forest. The results showed that the economic benefit of this type of forest is closely related to rotation and site class. Higher economic benefit could be obtained when the rotation is shorter and site class is higher. The planting density also had an obvious influence on economic benefit. On the base of assuring survival rate and conserving rate, the less the fee used in soil preparation and young growth tending is, the higher the economic benefit is. The influence of determined six cultivation meas-ures on economic benefit in sequence was the rotation-site class-density-management fee level-young growth tending in-tensity- soil preparation methods.

  11. Meshless numerical method based on tensor product

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    A normalized space constructed by tensor product is used in field function approach to give a special case of moving least squares (MLS) interpolation scheme.In the regular domain,the field function which meets homogenous boundary conditions is constructed by spanning base space to make the MLS interpolation scheme simpler and more efficient.Owing to expanded basis functions selection,some drawbacks in general MLS method,for example repeated inversion,low calculation efficiency,and complex criterions,can be avoided completely.Numerical examples illustrate that the proposed method is characterized by simple mathematical concept,convenient repeat calculations with high accuracy,good continuity,less computation and rapid convergence.

  12. Trinocular Calibration Method Based on Binocular Calibration

    Directory of Open Access Journals (Sweden)

    CAO Dan-Dan

    2012-10-01

    Full Text Available In order to solve the self-occlusion problem in plane-based multi-camera calibration system and expand the measurement range, a tri-camera vision system based on binocular calibration is proposed. The three cameras are grouped into two pairs, while the public camera is taken as the reference to build the global coordinate. By calibration of the measured absolute distance and the true absolute distance, global calibration is realized. The MRE (mean relative error of the global calibration of the two camera pairs in the experiments can be as low as 0.277% and 0.328% respectively. Experiment results show that this method is feasible, simple and effective, and has high precision.

  13. Kernel method-based fuzzy clustering algorithm

    Institute of Scientific and Technical Information of China (English)

    Wu Zhongdong; Gao Xinbo; Xie Weixin; Yu Jianping

    2005-01-01

    The fuzzy C-means clustering algorithm(FCM) to the fuzzy kernel C-means clustering algorithm(FKCM) to effectively perform cluster analysis on the diversiform structures are extended, such as non-hyperspherical data, data with noise, data with mixture of heterogeneous cluster prototypes, asymmetric data, etc. Based on the Mercer kernel, FKCM clustering algorithm is derived from FCM algorithm united with kernel method. The results of experiments with the synthetic and real data show that the FKCM clustering algorithm is universality and can effectively unsupervised analyze datasets with variform structures in contrast to FCM algorithm. It is can be imagined that kernel-based clustering algorithm is one of important research direction of fuzzy clustering analysis.

  14. On Task-based English Learning Method

    Institute of Scientific and Technical Information of China (English)

    朱蕾

    2010-01-01

    @@ Task-Based learning(TBL)is becoming a catchword in English circles.The new national English Curricular Syllabus also recommends the use of the TBL approach in classroom teaching.The purpose of learning a foreign language is the most direct communicative in the target language,and speaking is the most direct communicative method.In recent years,with the publication of the New Curriculum Standard by the State Education Department,the teaching reform in middle and primary schools has been being implemented step by step.

  15. Study on a pattern classification method of soil quality based on simplified learning sample dataset

    Science.gov (United States)

    Zhang, Jiahua; Liu, S.; Hu, Y.; Tian, Y.

    2011-01-01

    Based on the massive soil information in current soil quality grade evaluation, this paper constructed an intelligent classification approach of soil quality grade depending on classical sampling techniques and disordered multiclassification Logistic regression model. As a case study to determine the learning sample capacity under certain confidence level and estimation accuracy, and use c-means algorithm to automatically extract the simplified learning sample dataset from the cultivated soil quality grade evaluation database for the study area, Long chuan county in Guangdong province, a disordered Logistic classifier model was then built and the calculation analysis steps of soil quality grade intelligent classification were given. The result indicated that the soil quality grade can be effectively learned and predicted by the extracted simplified dataset through this method, which changed the traditional method for soil quality grade evaluation. ?? 2011 IEEE.

  16. 研究性课程中发明创造技法的应用及创新能力培养探讨%Application of Inventive Method and Cultivation of Innovative Ability in Research Programs

    Institute of Scientific and Technical Information of China (English)

    姜长阳; 徐娜; 张冬艳

    2012-01-01

    In order to cultivate the innovative ability of undergraduates, the students who took part in the research programs were encouraged to solved some problems in an innovative way. The problems concerning plant tissue culture and plant cloning which were difficult to deal with were successfully solved, which accelerated the development of research programs. 82 papers were published in less than 5 years, whose first authors were all undergraduates. With the application of inventive method,the innovative consciousness of the students could be awakened,the innovative thinking could be cultivated, the innovative ability could be enhanced.%为培养本科生的创新能力,让参加研究性课程的学生把发明创造技法应用于研学中,解决发现的问题.从而解决了植物组织培养、植物克隆中许多难以解决的问题,促进了研学的顺利进行,仅不到5年的时间,就取得了发表以本科生为第一作者的学术论文82篇等多项成果,并且通过发明创造技法在研学中的应用,也使学生树立创新意识,培养创新思维,提高创新能力.

  17. Effects of Temperature variations on the Super Fine Powderization of Korean Cultivated Wild Ginseng

    Directory of Open Access Journals (Sweden)

    Jin Ho Kim

    2006-12-01

    Full Text Available Objectives : The aim of this study was to find optimal conditions for producing red ginseng from cultivated wild ginseng using the Turbo Mill. Methods : Characteristics of powdered cultivated wild ginseng based on various temperature settings of the Turbo Mill were observed, and changes in the content was measured by HPLC for various ginsenosides. Results : 1. The diameter of cultivated wild ginseng powder ground by the Turbo Mill was around 10㎛. 2. As the temperature rose, presusre, Specific Mechanical Energy(SME, and density decreased, whileas Water Solubility Index(WSI increased. 3. As the temperature rose, super fine powder showed tendency to turn into dark brown. 4. Measuring content changes by HPLC, there was no detection of ginsenoside Rg3 and ginsenosideRg1, Rb1, and Rh2 concentrations decreased with increase in temperature. Conclusions : Super fine powder of cultivated wild ginseng produced by the Turbo Mill promotes easy absorption of effective ingredients by breaking the cell walls. Using this mechanism to produce red ginseng from cultivated wild ginseng, it yielded less than satisfactory results under the current experiment setup. Furtherresearches are needed to verify more suitable condition for the production of red ginseng.

  18. Bus Based Synchronization Method for CHIPPER Based NoC

    Directory of Open Access Journals (Sweden)

    D. Muralidharan

    2016-01-01

    Full Text Available Network on Chip (NoC reduces the communication delay of System on Chip (SoC. The main limitation of NoC is power consumption and area overhead. Bufferless NoC reduces the area complexity and power consumption by eliminating buffers in the traditional routers. The bufferless NoC design should include live lock freeness since they use hot potato routing. This increases the complexity of bufferless NoC design. Among the available propositions to reduce this complexity, CHIPPER based bufferless NoC is considered as one of the best options. Live lock freeness is provided in CHIPPER through golden epoch and golden packet. All routers follow some synchronization method to identify a golden packet. Clock based method is intuitively followed for synchronization in CHIPPER based NoCs. It is shown in this work that the worst-case latency of packets is unbearably high when the above synchronization is followed. To alleviate this problem, broadcast bus NoC (BBus NoC approach is proposed in this work. The proposed method decreases the worst-case latency of packets by increasing the golden epoch rate of CHIPPER.

  19. Comparative Study on the Human Driving Force of Cultivated Land and Construction Land Use Change in Hubei Province,China

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Change trend of cultivated land and construction land in Hubei Province is analyzed.The years 1998-2003 are the reduction period of cultivated land;the years 2004-2007 are the increase period of cultivated land;the years 1988-2002 are the slow growth period of construction land;and the years of 2003 and 2007 are the increase period of construction land.Based on related social economic statistical data in 1988-2007,human driving forces on cultivated land and construction land in Hubei Province are analyzed quantitatively and comparatively by using SPSS software and mathematical statistics method.Result shows that driving force factors have negative driving effect on cultivated land,and positive driving effect on construction land.Driving force factors have significant differences of driving effects on cultivated land and construction land.Three principal components affecting the changes of cultivated land and construction land are policy,population and economic development level,and gap between urban and rural living standards.The three principal components have different degrees of sensitivity on the two land use types and the effects direction are reverse.The other factor driving abilities are relatively weak,and these factors have relatively stronger driving force on construction land than that on cultivated land.It is put forward that Hubei Province should implement rational allocation of land resources and scientific and rational use in order to ensure land security and to realize the sustainable development of regional economy and society based on the intensive land use,the planning policy constraints,and the agricultural and industrial production.

  20. Building a field- and model-based climatology of local water and energy cycles in the cultivated Sahel - annual budgets and seasonality

    Science.gov (United States)

    Velluet, C.; Demarty, J.; Cappelaere, B.; Braud, I.; Issoufou, H. B.-A.; Boulain, N.; Ramier, D.; Mainassara, I.; Charvet, G.; Boucher, M.; Chazarin, J.-P.; Oï, M.; Yahou, H.; Maidaji, B.; Arpin-Pont, F.; Benarrosh, N.; Mahamane, A.; Nazoumou, Y.; Favreau, G.; Seghieri, J.

    2014-12-01

    In the sub-Saharan Sahel, energy and water cycling at the land surface is pivotal for the regional climate, water resources and land productivity, yet it is still very poorly documented. As a step towards a comprehensive climatological description of surface fluxes in this area, this study provides estimates of long-term average annual budgets and seasonal cycles for two main land use types of the cultivated Sahelian belt: rainfed millet crop and fallow bush. These estimates build on the combination of a 7-year field data set from two typical plots in southwestern Niger with detailed physically based soil-plant-atmosphere modeling, yielding a continuous, comprehensive set of water and energy flux and storage variables over this multiyear period. In the present case in particular, blending field data with mechanistic modeling makes the best use of available data and knowledge for the construction of the multivariate time series. Rather than using the model only to gap-fill observations into a composite series, model-data integration is generalized homogeneously over time by generating the whole series with the entire data-constrained model simulation. Climatological averages of all water and energy variables, with associated sampling uncertainty, are derived at annual to sub-seasonal scales from the time series produced. Similarities and differences in the two ecosystem behaviors are highlighted. Mean annual evapotranspiration is found to represent ~82-85% of rainfall for both systems, but with different soil evaporation/plant transpiration partitioning and different seasonal distribution. The remainder consists entirely of runoff for the fallow, whereas drainage and runoff stand in a 40-60% proportion for the millet field. These results should provide a robust reference for the surface energy- and water-related studies needed in this region. Their significance and the benefits they gain from the innovative data-model integration approach are thoroughly discussed

  1. 大学生创业能力研究及其培养%Study on College Students' Entrepreneurship Ability and Its Cultivation Based on Professional Skills Elements

    Institute of Scientific and Technical Information of China (English)

    刘畅

    2011-01-01

    从"职业能力"的研究角度,确定了大学生创业能力的能力要素,并在分析大学生创业能力培养环境的基础上,阐述了大学生创业能力的培养路径,旨在推动"国家、高校、大学生"三位一体的大学生创业软环境的日趋完善。%This paper starts from the significance of college students' entrepreneurship ability cultivation,analyzing the ability elements of college student's entrepreneurship from the research aspect of vocation ability.It also states the cultivation approach of college students' entrepreneurship ability,aiming to improve the college students' entrepreneurship soft environment of the trinity of country,college and students.

  2. 以化学竞赛为契机 着力培养应用型人才%Cultivating Applied Talents Based on Chemistry Competition

    Institute of Scientific and Technical Information of China (English)

    韦寿莲; 汪洪武; 许秀丽; 刘玲; 严子军; 赵建芬

    2011-01-01

    Owing to a large social demand for applied talents, combining the school orientation, the cultivation target and an awareness of chemistry competition among students, it' s studied on how to optimize the teaching resources, to improve the teaching quality and to promote the cultivation of applied talents through chemistry competitions in local colleges.%基于社会对应用型人才的大量需求,结合肇庆学院的办学定位、培养目标及化学化工学院学生对竞赛的认知,研究地方高校如何通过化学竞赛优化教学资源,提高教学质量,促进应用型人才的培养.

  3. Mushroom Cultivation in South Korea

    OpenAIRE

    Mustafa Kemal Soylu; Mingu Kang

    2016-01-01

    Mushroom cultivation in South Korea is increasing fast last decades. Mushroom cultivation of South Korea is 173577 tones and South Korea gains 800 million dollars income annually. Different kind of mushroom species are cultivated and 31% enoki mushroom (Flammulina velutipes), 26% king oyster (Pleurotus eryngii), 26% oyster mushroom (Pleurotus ostreatus), 13% white buton mushroom (Agaricus bisporus) and 4% rest of the total mushroom Lentinula edodes, Ganoderma lucidum, Phellinus vb. are produc...

  4. Rice Cultivation Methods and Their Sustainability Aspects: Organic and Conventional Rice Production in Industrialized Tropical Monsoon Asia with a Dual Cropping System

    Directory of Open Access Journals (Sweden)

    Hung-Chun Lin

    2016-06-01

    Full Text Available Options to tackle the sustainability challenges faced in the production of rice, including global and local environmental perspectives, need to be discussed. Here, the global warming potential, water consumption and cumulative energy demand were analyzed using a life-cycle assessment to highlight the sustainability aspects of rice production in Taiwan, where a mixed organic and conventional rice production with a dual cropping system is practiced. The results show that the conventional farming method practiced in Houbi district contributes less to global warming and annual water consumption and consumes less energy than the organic method practiced in Luoshan village on a grain weight basis. It is also more lucrative for farmers because of the higher rice yield. Considering the yield ratio based on the data from two districts, the regional characteristics are more responsible for these differences. Giving up dual cropping to avail water to other sectors by fallowing during the second cropping season is preferable from the GHG emission and productivity perspectives. However, because water shortages usually occur in the first cropping season, it is more realistic to fallow during the first cropping season when domestic and other industrial users have the higher priority. The results presented here can serve as the foundation for exploring the possibilities of options, such as new biorefinery technologies and water allocation policies, in relation to influences on GHG emissions and the national self-sufficiency of rice.

  5. Evidence for a Common Origin of Blacksmiths and Cultivators in the Ethiopian Ari within the Last 4500 Years: Lessons for Clustering-Based Inference.

    OpenAIRE

    Lucy van Dorp; David Balding; Simon Myers; Luca Pagani; Chris Tyler-Smith; Endashaw Bekele; Ayele Tarekegn; Thomas, Mark G; Neil Bradman; Garrett Hellenthal

    2015-01-01

    The Ari peoples of Ethiopia are comprised of different occupational groups that can be distinguished genetically, with Ari Cultivators and the socially marginalised Ari Blacksmiths recently shown to have a similar level of genetic differentiation between them (FST ≈ 0.023 - 0.04) as that observed among multiple ethnic groups sampled throughout Ethiopia. Anthropologists have proposed two competing theories to explain the origins of the Ari Blacksmiths as (i) remnants of a population that inhab...

  6. Pilot scale land-based cultivation of Saccharina latissima Linnaeus at southern European climate conditions: Growth and nutrient uptake at high temperatures

    DEFF Research Database (Denmark)

    Azevedo, Isabel C.; Silva Marinho, Goncalo; Silva, Diogo M.;

    2016-01-01

    for this species. This fact may be explained by their origin in populations located near the southern distribution boundary, which may have acquired adaptations that increased tolerance to high temperatures. Cultivation of S. latissima using tumble culture in outdoor tanks at southern latitudes...... appears to be feasible even during high temperature periods. Densities around 8 kg m− 3 were effective in keeping epiphytes development low. This system may be used for seaweed monoculture or as a biofilter component of IMTA systems....

  7. Surface modification of closed plastic bags for adherent cell cultivation

    Science.gov (United States)

    Lachmann, K.; Dohse, A.; Thomas, M.; Pohl, S.; Meyring, W.; Dittmar, K. E. J.; Lindenmeier, W.; Klages, C.-P.

    2011-07-01

    In modern medicine human mesenchymal stem cells are becoming increasingly important. However, a successful cultivation of this type of cells is only possible under very specific conditions. Of great importance, for instance, are the absence of contaminants such as foreign microbiological organisms, i.e., sterility, and the chemical functionalization of the ground on which the cells are grown. As cultivation of these cells makes high demands, a new procedure for cell cultivation has been developed in which closed plastic bags are used. For adherent cell growth chemical functional groups have to be introduced on the inner surface of the plastic bag. This can be achieved by a new, atmospheric-pressure plasma-based method presented in this paper. The method which was developed jointly by the Fraunhofer IST and the Helmholtz HZI can be implemented in automated equipment as is also shown in this contribution. Plasma process gases used include helium or helium-based gas mixtures (He + N2 + H2) and vapors of suitable film-forming agents or precursors such as APTMS, DACH, and TMOS in helium. The effect of plasma treatment is investigated by FTIR-ATR spectroscopy as well as surface tension determination based on contact angle measurements and XPS. Plasma treatment in nominally pure helium increases the surface tension of the polymer foil due to the presence of oxygen traces in the gas and oxygen diffusing through the gas-permeable foil, respectively, reacting with surface radical centers formed during contact with the discharge. Primary amino groups are obtained on the inner surface by treatment in mixtures with nitrogen and hydrogen albeit their amount is comparably small due to diffusion of oxygen through the gas-permeable bag, interfering with the plasma-amination process. Surface modifications introducing amino groups on the inner surface turned out to be most efficient in the promotion of cell growth.

  8. PERFORMANCE OF TRANSPLANTED SCENTED RICE (ORYZA SATIVA L. UNDER SRI BASED CULTIVATION PRACTICES; A SUSTAINABLE METHOD FOR CROP PRODUCTION

    Directory of Open Access Journals (Sweden)

    DAMINI THAWAIT

    2014-06-01

    Full Text Available The experiment was carried out at Raipur during season of 2012 to find out the performance of transplanted scented rice under different planting geometries and seedling densities. The treatment (T2 i.e. planting of 2-3 seedlings hill-1 transplanted in the spacing of 25 cm x 25 cm recorded significantly highest i.e. Plant height (129.64cm, number of tillers (15.70 tillers hill-1, dry matter accumulation (102.65 g hill-1 and yield attributing characters along with highest grain yield i.e. 38.20 q/ha and straw yield ( 77.91 q/ha. Therefore its adoption protects the ecosystem as well as increasing production also.

  9. PERFORMANCE OF TRANSPLANTED SCENTED RICE (ORYZA SATIVA L.) UNDER SRI BASED CULTIVATION PRACTICES; A SUSTAINABLE METHOD FOR CROP PRODUCTION

    OpenAIRE

    DAMINI THAWAIT; Patel, Amit K.; SAMAPTIKA KAR; MANISH KUMAR SHARMA; MAYUR R. MESHRAM

    2014-01-01

    The experiment was carried out at Raipur during season of 2012 to find out the performance of transplanted scented rice under different planting geometries and seedling densities. The treatment (T2 ) i.e. planting of 2-3 seedlings hill-1 transplanted in the spacing of 25 cm x 25 cm recorded significantly highest i.e. Plant height (129.64cm), number of tillers (15.70 tillers hill-1), dry matter accumulation (102.65 g hill-1) and yield attributing characters along with highest gr...

  10. Identification of Growth Phases and Influencing Factors in Cultivations with AGE1.HN Cells Using Set-Based Methods

    OpenAIRE

    Borchers, S.; Freund, S; Rath, A.; Streif, S; Reichl, U.; Findeisen, R.

    2013-01-01

    Production of bio-pharmaceuticals in cell culture, such as mammalian cells, is challenging. Mathematical models can provide support to the analysis, optimization, and the operation of production processes. In particular, unstructured models are suited for these purposes, since they can be tailored to particular process conditions. To this end, growth phases and the most relevant factors influencing cell growth and product formation have to be identified. Due to noisy and erroneous experimenta...

  11. Graph-based Methods for Orbit Classification

    Energy Technology Data Exchange (ETDEWEB)

    Bagherjeiran, A; Kamath, C

    2005-09-29

    An important step in the quest for low-cost fusion power is the ability to perform and analyze experiments in prototype fusion reactors. One of the tasks in the analysis of experimental data is the classification of orbits in Poincare plots. These plots are generated by the particles in a fusion reactor as they move within the toroidal device. In this paper, we describe the use of graph-based methods to extract features from orbits. These features are then used to classify the orbits into several categories. Our results show that existing machine learning algorithms are successful in classifying orbits with few points, a situation which can arise in data from experiments.

  12. Research on Monitoring Area Division of Quality Grade Changes in County Cultivated Land and Technology of Deploying Monitoring Point

    OpenAIRE

    Wei, Wei; Liao, Lijun; Yu, Jianxin

    2013-01-01

    It is an important means in management of improving both the quality and quantity of cultivated land to monitor grade changes in cultivated land quality. How to deploy monitoring network system and its point reasonably and roundly are the key to the technology of monitoring grade changes in cultivated land quality by monitoring grade changes in cultivated land quality dynamically in order to obtain the information to the index of cultivated land quality and its changes based on the existing a...

  13. Data Driven Modeling for Monitoring and Control of Industrial Fed-Batch Cultivations

    DEFF Research Database (Denmark)

    Bonné, Dennis; Alvarez, María Antonieta; Jørgensen, Sten Bay

    2014-01-01

    A systematic methodology for development of a set of discrete-time sequence models for batch control based on historical and online operating data is presented and investigated experimentally. The modeling is based on the two independent characteristic time dimensions of batch processing, being t...... optimization of the bioreactor operations model. The modeling and preliminary control performance is demonstrated on an industrial fed-batch protein cultivation production process. The presented methods lend themselves directly for application as Process Analytical Technologies....

  14. Study on Economic growth, Urbanization and Sustainable Utilization of Cultivated Land in China

    Institute of Scientific and Technical Information of China (English)

    Wu Qun; Guo Guancheng; Fan Li

    2004-01-01

    By means of the dynamic regression model, this paper analyzes the relationships among economic growth, urbanization and changes of cultivated land in China, finds that the ratio of cultivated land occupied by economic growth is decreasing with social and economic growth. And,based on that, some policy suggestions on how to promote the sustainable use of cultivated land in China are put forward.

  15. Functional State Modelling of Cultivation Processes: Dissolved Oxygen Limitation State

    Directory of Open Access Journals (Sweden)

    Olympia Roeva

    2015-04-01

    Full Text Available A new functional state, namely dissolved oxygen limitation state for both bacteria Escherichia coli and yeast Saccharomyces cerevisiae fed-batch cultivation processes is presented in this study. Functional state modelling approach is applied to cultivation processes in order to overcome the main disadvantages of using global process model, namely complex model structure and a big number of model parameters. Alongwith the newly introduced dissolved oxygen limitation state, second acetate production state and first acetate production state are recognized during the fed-batch cultivation of E. coli, while mixed oxidative state and first ethanol production state are recognized during the fed-batch cultivation of S. cerevisiae. For all mentioned above functional states both structural and parameter identification is here performed based on experimental data of E. coli and S. cerevisiae fed-batch cultivations.

  16. Glass bead cultivation of fungi

    DEFF Research Database (Denmark)

    Droce, Aida; Sørensen, Jens Laurids; Giese, H.;

    2013-01-01

    Production of bioactive compounds and enzymes from filamentous fungi is highly dependent on cultivation conditions. Here we present an easy way to cultivate filamentous fungi on glass beads that allow complete control of nutrient supply. Secondary metabolite production in Fusarium graminearum...

  17. Cultivating Technological Innovation in the Mind

    Directory of Open Access Journals (Sweden)

    PROF ANYATA BENEDICT U.(Ph.D

    2015-11-01

    Full Text Available Cultivating innovation in the mind is imperative to successfully move technology to the next level. Technology is growing rapidly in developed countries and has almost reached its peak. Developing countries and continents like Africa on the other hand are now seen as a breeding ground to explore in technological innovation. Researchers and entrepreneurs are shifting base to Africa where they can achieve maximum profit resulting from under development of the continent. Reefs, city and web are used to explain the best way to cultivate innovation. The city and web are such engines of new innovation because both environments are powerfully suited for the creation, diffusion and adoption of good ideas. The seven keys of cultivating innovation include tropical humidity, illiteracy, poor power surge of electronic use for research, good concept of the liquid networks, slow hunches, serendipity, acceptance of error in cultivating innovation, Exaptation, coffee breaks, application of programming interface and the use of quadrant as a tool. These keys are recommended to be put to use by every individual who has the mindset of driving technological innovation to the next level.

  18. Mathematic Teaching Strategy Based on Teamwork Spirit Cultivation%基于合作意识培养的数学教学策略探析

    Institute of Scientific and Technical Information of China (English)

    雷兴辉

    2011-01-01

    针对以就业为导向的职业院校数学教学,探讨在教学中培养学生合作意识和合作能力的策略,以期为学生就业做好数学方面的准备.%Aiming at employment-oriented mathematic teaching in vocational college, the article explored the strategy of cultivating students' teamwork spirit and team work ability in teaching, in order to prepare for students' employment.

  19. On Cultivating Students' Motivation in Second Language Acquisition

    Institute of Scientific and Technical Information of China (English)

    沈小平

    2009-01-01

    This paper attempts to explore the cultivation of motivation in second language acquisition based on shedding new light on the definition, the importance, and the classification of learners' motivation in second language acquisition.

  20. High throughput Single-cell Cultivation on Microfluidic Streak Plates

    OpenAIRE

    Jiang, Cheng-Ying; Dong, Libing; Zhao, Jian-Kang; Hu, Xiaofang; Shen, Chaohua; Qiao, Yuxin; Zhang, Xinyue; Wang, Yapei; Ismagilov, Rustem F.; Liu, Shuang-Jiang; Du, Wenbin

    2016-01-01

    This paper describes the microfluidic streak plate (MSP), a facile method for high-throughput microbial cell separation and cultivation in nanoliter sessile droplets. The MSP method builds upon the conventional streak plate technique by using microfluidic devices to generate nanoliter droplets that can be streaked manually or robotically onto petri dishes prefilled with carrier oil for cultivation of single cells. In addition, chemical gradients could be encoded in the droplet array for compr...

  1. Cultivo de Oncidium baueri Lindley (Orchidaceae) em substratos a base de coco Oncidium baueri Lindley (Orchidaceae) cultivation in coconut-based substrates

    OpenAIRE

    Adriane Marinho de Assis; Ricardo Tadeu Faria; Lilian Keiko Unemoto; Larissa Abgariani Colombo

    2008-01-01

    Entre os substratos utilizados no cultivo de orquídeas, a fibra de coco vem se destacando como promissor substituto do xaxim, material utilizado por muitos produtores e colecionadores de orquídeas, porém ameaçado de extinção. Objetivou-se, neste trabalho, foi avaliar a eficiência de substratos à base de coco, no cultivo da orquídea Oncidium baueri. As mudas foram cultivadas em vasos de polipropileno, permanecendo em viveiro com 50% de luminosidade. Os tratamentos constituíram-se de xaxim desf...

  2. Region-based multisensor image fusion method

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Image fusion should consider the priori knowledge of the source images to be fused, such as the characteristics of the images and the goal of image fusion, that is to say, the knowledge about the input data and the application plays a crucial role. This paper is concerned on multiresolution (MR) image fusion. Considering the characteristics of the multisensor (SAR and FLIR etc) and the goal of fusion, which is to achieve one image in possession of the contours feature and the target region feature. It seems more meaningful to combine features rather than pixels. A multisensor image fusion scheme based on K-means cluster and steerable pyramid is presented. K-means cluster is used to segment out objects in FLIR images. The steerable pyramid is a multiresolution analysis method, which has a good property to extract contours information at different scales. Comparisons are made with the relevant existing techniques in the literature. The paper concludes with some examples to illustrate the efficiency of the proposed scheme.

  3. IN-SITU DETERMINATION OF SEDIMENT OXYGEN DEMAND IN CULTIVATION PONDS

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    This paper presents a method for in-situ determining sediment oxygen demand (SOD) in cultivation pond. This method based on sediment surface structure, temperature, and other determining conditions like those in shrimp cultivation environments, overcomes defects of old methods and provides more accurate estimation of SOD's effect on dissolved oxygen in culture waters. Our experiment shows that the sediment surface structure and temperature had important effect on SOD in culture water. Different SOD values were derived from different parts of oxygen consumption curves of sediment, because the curves were not linear. According to the oxygen consumption curves of sediment and saturated DO in culture water, it was thought more suitable to calculate SOD with dissolved oxygen reduction from 5.0 to 2.0 mg/l. This method to determine the SOD of shrimp ponds yielded satisfactory results.

  4. Labor Cost Analysis for Pome Production in Different Cultivation Modes in Hebei Province

    Institute of Scientific and Technical Information of China (English)

    Shaoyan; ZHOU; Yu; HUANG; Cui; YAO; Runhang; LU; Xingfang; LIU

    2013-01-01

    Taking the traditional fruit pear as the example, this paper analyzes labor cost for pome production in Hebei Province, based on the representative cases and the research of pome production in different cultivation modes. Firstly, it conducts cost analysis for medium-density pome production in Xinji City, focusing on the comparison of the costs for the main production labor in standard thin planting mode and dwarf close planting mode. According to the research results, labor cost has a great influence on the total production cost of pome. The methods to reduce labor cost include: adopting dwarfing rootstock close planting and intensively efficient pome cultivation method; simplifying the pruning method when matching up the shape of tree; improving soil by the methods of natural grasses and addition of organic materials, and increasing mechanized operation.

  5. 基于四网融合的产业升级与新兴产业培育研究%Industry Upgrade and New Industry Cultivation Based on Four Synergetic Networks

    Institute of Scientific and Technical Information of China (English)

    孙德忠; 周荣; 喻登科

    2014-01-01

    The abundant connotations of value network ,knowledge network ,social network and internet of things are tried out ,and their reciprocities are analyzed ,and then the four networks synchronized mechanism is researched based on the two-two fusion .The industry upgrade and new industry cultivation mechanism based on four synergetic networks are dis-cussed from essence ,mechanism and process .Two industry upgrade modes including grandness and upgrade ,and four new industry cultivation modes including gap padding ,network complementary ,service intensifying and efficiency leading are put forward .These can provide a new development theory for industry upgrade and new industry cultivation of China .%提炼出了价值网、知识网、社会网和物联网的丰富内涵,剖析了它们之间的相互作用关系,从两两融合的角度研究了四网融合机理。进而从本质、机制和过程3个层面探讨了基于四网融合的产业升级与新兴产业培育机理,提出了四网融合下壮大式和升级式两种产业升级模式,以及缝隙填补、网络互补、服务强化、效率领先等4种新兴产业培育模式,为我国产业升级和新兴产业培育提供了一种崭新的发展理念。

  6. 高职酒店管理专业学生职业素质培养研究-基于中原经济区建设背景%On the Cultivation of Occupational Qualities of Hotel Management Students in Vocational Colleges:Based on the Construction Background of the Central Plains Economic Zone

    Institute of Scientific and Technical Information of China (English)

    张志强

    2014-01-01

    在中原经济区的建设大背景下,酒店业迎来了新的发展机遇,但是酒店管理专业人才的职业素养现状与人才需求之间仍有较大差距。为培养酒店高素质服务型人才,助推中原经济区建设,针对目前酒店业人才需求现状和高职人才培养现状,从人才培养定位、课程与教学体系改革、校企合作等方面提出了高职酒店管理专业学生职业素质培养的途径。%In the background of the construction of the Central Plains Economic Zone, the hotel business is facing new opportunities for development, but there is still a wide gap between the hotel management students’ professionalism and talent demand of hotel management. In order to cultivate high-quality hotel services professionals, boosting the economic development of the Central Plains region, based on the current hotel industry demand and the present situation of higher vocational talent training, the article puts forward higher vocational hotel management professional quality development methods to cultivate students’ professional quality, which range from talents cultivation orientation, curriculum and teaching system reform, to university-enterprise cooperation and so on.

  7. On the Cultivation of Occupational Qualities of Hotel Management Students in Vocational Colleges:Based on the Construction Background of the Central Plains Economic Zone%高职酒店管理专业学生职业素质培养研究-基于中原经济区建设背景

    Institute of Scientific and Technical Information of China (English)

    张志强

    2014-01-01

    In the background of the construction of the Central Plains Economic Zone, the hotel business is facing new opportunities for development, but there is still a wide gap between the hotel management students’ professionalism and talent demand of hotel management. In order to cultivate high-quality hotel services professionals, boosting the economic development of the Central Plains region, based on the current hotel industry demand and the present situation of higher vocational talent training, the article puts forward higher vocational hotel management professional quality development methods to cultivate students’ professional quality, which range from talents cultivation orientation, curriculum and teaching system reform, to university-enterprise cooperation and so on.%在中原经济区的建设大背景下,酒店业迎来了新的发展机遇,但是酒店管理专业人才的职业素养现状与人才需求之间仍有较大差距。为培养酒店高素质服务型人才,助推中原经济区建设,针对目前酒店业人才需求现状和高职人才培养现状,从人才培养定位、课程与教学体系改革、校企合作等方面提出了高职酒店管理专业学生职业素质培养的途径。

  8. CONTENT AND UPTAKE OF SELECTED TRACE ELEMENTS BY WEEDS IN POTATO TO CULTIVATION UNDER DIFFERENT CONDITIONS OF SOIL TILLAGE AND WEED CONTROL METHODS

    OpenAIRE

    Krystyna Zarzecka; Marek Gugała; Alicja Baranowska

    2014-01-01

    The study utilized data from a field experiment carried out at the Experimental Station in Zawady owned by the University of Natural Sciences and Humanities in Siedlce in the years 2005–2007. The experimental factors included two soil tillage systems and seven weed control methods in potato. Iron, copper and zinc in weeds were determined with the AAS method. The trace element content in weed dry matter before row closure of potato depended significantly on soil tillage methods (excluding Cu),...

  9. Classification and Gradation of Cultivated Land Quality in Bishan County of Chongqing, China

    Institute of Scientific and Technical Information of China (English)

    SHAO Jing'an; GE Xiaofeng; WEI Chaofu; XIE Deti

    2007-01-01

    The conflicts among food security, economic development and ecological protection are the "sticking point"of undeveloped southwestern mountainous areas of China. The objectives of this study are to identify appropriate integrated indicators influencing the classification and gradation of cultivated land quality in the southwestern mountainous area of China based on semi-structure interview, and to promote the monitoring of cultivated land quality in this region.Taking Bishan County of Chongqing as a study case, the integrated indicators involve the productivity, protection, acceptability, and stability of cultivated land. The integrated indicators accord with the characteristics of land resources and human preference in southwestern mountainous area of China. In different agricultural zones, we emphasize different indicators, such as emphasizing productivity, stabilization and acceptability in low hilly and plain agricultural integrative zone (LHP-AIZ), protection, productivity and stability in low mountain and hill agro-forestry ecological zone (LMH-AEZ), and acceptability in plain outskirts integrative agricultural zone (PO-IAZ), respectively. The pronounced difference of classification and gradation of cultivated land, regardless of inter-region or intra-region, is observed, with the reducible rank from PO-IAZ, LHP-AIZ to LMH-AEZ. Research results accord with the characteristics of assets management and intensive utilization of cultivated land resources in the southwestern mountainous area of China.Semi-structure interview adequately presents the principal agent of farmers in agricultural land use and rural land market.This method is very effective and feasible to obtain data of the quality of cultivated land in the southwestern mountainous area of China.

  10. Deforestation and cultivation mobilize mercury from topsoil.

    Science.gov (United States)

    Gamby, Rebecca L; Hammerschmidt, Chad R; Costello, David M; Lamborg, Carl H; Runkle, James R

    2015-11-01

    Terrestrial biomass and soils are a primary global reservoir of mercury (Hg) derived from natural and anthropogenic sources; however, relatively little is known about the fate and stability of Hg in the surface soil reservoir and its susceptibility to change as a result of deforestation and cultivation. In southwest Ohio, we measured Hg concentrations in soils of deciduous old- and new-growth forests, as well as fallow grassland and agricultural soils that had once been forested to examine how, over decadal to century time scales, man-made deforestation and cultivation influence Hg mobility from temperate surface soils. Mercury concentrations in surficial soils were significantly greater in the old-growth than new-growth forest, and both forest soils had greater Hg concentrations than cultivated and fallow fields. Differences in Hg:lead ratios between old-growth forest and agricultural topsoils suggest that about half of the Hg lost from deforested and cultivated Ohio soils may have been volatilized and the other half eroded. The estimated mobilization potential of Hg as a result of deforestation was 4.1 mg m(-2), which was proportional to mobilization potentials measured at multiple locations in the Amazon relative to concentrations in forested surface soils. Based on this relationship and an estimate of the global average of Hg concentrations in forested soils, we approximate that about 550 M mol of Hg has been mobilized globally from soil as a result of deforestation during the past two centuries. This estimate is comparable to, if not greater than, the amount of anthropogenic Hg hypothesized by others to have been sequestered by the soil reservoir since Industrialization. Our results suggest that deforestation and soil cultivation are significant anthropogenic processes that exacerbate Hg mobilization from soil and its cycling in the environment. PMID:26100725

  11. Cultivation of microalgae in industrial wastewaters

    DEFF Research Database (Denmark)

    van Wagenen, Jonathan Myerson

    to CO2. However, various anaerobic treatment methods would result in effluent containing dissolved organic molecules suitable for algae species that have the ability to grow as mixo- or heterotrophs. Chlorella sorokiniana was cultivated in a lab scale photobioreactor under daily light dark cycles and...... good algae growth; knowledge about the mixotrophic utilization of chemical energy present in organic waste; demonstration of a method to optimize efficiency of culture growth and nutrient removal; and biochemical characterization of the produced biomass. When designing algae cultivation, one challenge...... various timing strategies were tested for adding acetate at concentrations that can be obtained in waste streams of 1 – 2 g L-1. The results showed that the fastest growth occurred when adding the acetate at night (cyclic autotrophy/heterotrophy). However adding the acetate during the day (mixotrophty...

  12. Triptycene-based ladder monomers and polymers, methods of making each, and methods of use

    KAUST Repository

    Pinnau, Ingo

    2015-02-05

    Embodiments of the present disclosure provide for a triptycene-based A-B monomer, a method of making a triptycene-based A-B monomer, a triptycene-based ladder polymer, a method of making a triptycene-based ladder polymers, a method of using triptycene-based ladder polymers, a structure incorporating triptycene-based ladder polymers, a method of gas separation, and the like.

  13. APPLICATION DIMENSIONAL AND SIMILARITY THEORY IN DETERMINING THE PARAMETERS AND OPERATING MODES OF SOIL CULTIVATING MACHINES

    Directory of Open Access Journals (Sweden)

    Shhirov V. N.

    2015-06-01

    Full Text Available The article presents a study of parameters and modes of operation of machines for soil cultivation. In determining the parameters and modes of operation of machinery for tillage we have applied the theory of similarity and dimensions of physical quantities. We have obtained the regularities of disclosing the relationship of the parameters from the medium to the energy characteristics of the process. As the initial data we used test protocols of machines for soil cultivation (Central - Black Earth, Kubanskaya, Sibirskaya, of North - Caucasion MIS, RosNIITiM : KPI - 3.8, AРC - 3.9, AKV - 4, AKM - 6 - V, AMP - 4 APC - 4 A, AРC - 10 APR - 4.4, APU - 6.5 APSH - 6 , CNC - 6.0, CSТ - 3.8, APC - 4. We defined the formula оf dimension parameters and modes of operation of machines for soil cultivation and properties of soil (traction resistance, depth, width, speed, hardness of the soil, acceleration. Based on dimension theory we have received similarity criteria. Based on the correlation analysis and the least squares method we determined the nature of addiction and the coefficients for it. We have also received a graph for determining the operating modes and parameters of machines for soil cultivation

  14. A study on the comparison of antioxidant effects among wild ginseng, cultivated wild ginseng, and cultivated ginseng extracts

    Directory of Open Access Journals (Sweden)

    Hae Young, Jang

    2008-09-01

    Full Text Available Objective : The objective of this study was to compare the antioxidant effects among wild ginseng, cultivated wild ginseng, and ginseng extracts. Methods : In vitro antioxidant activities were examined by total antioxidant capacity (TAC, oxygen radical scavenging capacity(ORAC, total phenolic content, 1, 1-Diphenyl-2-picrylhydrazyl (DPPH radical scavenging activity, inhibition of induced lipid peroxidation using liver mitochondria, reactive oxygen species(ROS scavenging effect using 2’, 7’-dichlorofluorescein(DCF fluorescence. Results : 1. TAC of 1.5 and 3.75 mg extracts was highest in cultivated wild ginseng, followed by wild ginseng and lowest in ginseng. 2. ORAC of 2, 10, and 20 μg extracts was highest in cultivated wild ginseng, followed by wild ginseng and lowest in ginseng. 3. Total phenolic content of 0.375, 0.938, and 1.875 mg extracts was highest in cultivated wild ginseng, followed by wild ginseng and lowest in ginseng. 4. DPPH(1, 1 -Diphenyl-2-picrylhydrazyl scavenging activity between wild ginseng and cultivated wild ginseng did not differ significantly (p>0.05. 5. Induced lipid peroxidation, measured by TBARS concentration in solution containing rat liver mitochondria incubated in the presence of FeSO4/ascorbic acid was inhibited as amounts of wild ginseng, cultivated wild ginseng, and ginseng extracts increased. TBARS concentration of ginseng extracts were significantly (p<0.05 higher than wild ginseng or cultivated wild ginseng extracts. 6. DCF fluorescence intensity was decreased as concentrations of wild ginseng, cultivated wild ginseng, and ginseng extracts increased, demonstrating that ROS generation was inhibited in a concentrationdependent manner. Conclusions : In summary, the results of this study demonstrate that cultivated wild ginseng extracts had similar antioxidant activities to wild ginseng extracts and greater that of cultivated ginseng extracts.

  15. New deghosting method based on generalized triangulation

    Institute of Scientific and Technical Information of China (English)

    Bai Jing; Wang Guohong; Xiu Jianjuan; Wang Xiaobo

    2009-01-01

    A new deghosting method baaed on the generalized triangulation is presented. First, two intersection points corresponding to the emitter position are obtained by utilizing two azimuth angles and two elevation angles from two jammed 3-D radars (or 2-D passive sensors). Then, hypothesis testing baaed deghosting method in the multiple target scenarios is proposed using the two intersection points. In order to analyze the performance of the proposed method, the correct association probability of the true targets and the incorrect association probability of the ghost targets are defined. Finally, the Monte Carlo simulations are given for the proposed method compared with the hinge angle method in the cases of both two and three radars. The simulation results show that the proposed method has better performance than the hinge angle method in three radars case.

  16. Development and application of a quantitative method based on LC-QqQ MS/MS for determination of steviol glycosides in Stevia leaves.

    Science.gov (United States)

    Molina-Calle, M; Sánchez de Medina, V; Delgado de la Torre, M P; Priego-Capote, F; Luque de Castro, M D

    2016-07-01

    Stevia is a currently well-known plant thanks to the presence of steviol glycosides, which are considered as sweeteners obtained from a natural source. In this research, a method based on LC-MS/MS by using a triple quadrupole detector was developed for quantitation of 8 steviol glycosides in extracts from Stevia leaves. The ionization and fragmentation parameters for selected reaction monitoring were optimized. Detection and quantitation limits ranging from 0.1 to 0.5ng/mL and from 0.5 to 1ng/mL, respectively, were achieved: the lowest attained so far. The steviol glycosides were quantified in extracts from leaves of seven varieties of Stevia cultivated in laboratory, greenhouse and field. Plants cultivated in field presented higher concentration of steviol glycosides than those cultivated in greenhouse. Thus, the way of cultivation clearly influences the concentration of these compounds. The inclusion of branches together with leaves as raw material was also evaluated, showing that this inclusion modifies, either positively or negatively, the concentration of steviol glycosides. PMID:27154673

  17. Soil protection through almond tree cultivation

    International Nuclear Information System (INIS)

    Most threat to soil are particularly severe in areas with steps slopes and suffering dry periods followed by heavy rain such as the Mediterranean regions. Severity is aggravated by lacking or inappropriate farming systems. Therefore the objective of this work was to demonstrate that land management based on cultivation of new varieties of local crops (almond trees) suited to these conditions may result in a sustainable system to prevent soil degradation. (Author)

  18. Enzyme controlled glucose auto-delivery for high cell density cultivations in microplates and shake flasks

    Directory of Open Access Journals (Sweden)

    Casteleijn Marco G

    2008-11-01

    Full Text Available Abstract Background Here we describe a novel cultivation method, called EnBase™, or enzyme-based-substrate-delivery, for the growth of microorganisms in millilitre and sub-millilitre scale which yields 5 to 20 times higher cell densities compared to standard methods. The novel method can be directly applied in microwell plates and shake flasks without any requirements for additional sensors or liquid supply systems. EnBase is therefore readily applicable for many high throughput applications, such as DNA production for genome sequencing, optimisation of protein expression, production of proteins for structural genomics, bioprocess development, and screening of enzyme and metagenomic libraries. Results High cell densities with EnBase are obtained by applying the concept of glucose-limited fed-batch cultivation which is commonly used in industrial processes. The major difference of the novel method is that no external glucose feed is required, but glucose is released into the growth medium by enzymatic degradation of starch. To cope with the high levels of starch necessary for high cell density cultivation, starch is supplied to the growing culture suspension by continuous diffusion from a storage gel. Our results show that the controlled enzyme-based supply of glucose allows a glucose-limited growth to high cell densities of OD600 = 20 to 30 (corresponding to 6 to 9 g l-1 cell dry weight without the external feed of additional compounds in shake flasks and 96-well plates. The final cell density can be further increased by addition of extra nitrogen during the cultivation. Production of a heterologous triosphosphate isomerase in E. coli BL21(DE3 resulted in 10 times higher volumetric product yield and a higher ratio of soluble to insoluble product when compared to the conventional production method. Conclusion The novel EnBase method is robust and simple-to-apply for high cell density cultivation in shake flasks and microwell plates. The

  19. Communication Error Analysis Method based on CREAM

    International Nuclear Information System (INIS)

    Communication error has been considered as a primary reason of many incidents and accidents in nuclear industry. In order to prevent these accidents, an analysis method of communication errors is proposed. This study presents a qualitative method to analyze communication errors. The qualitative method focuses on finding a root cause of the communication error and predicting the type of communication error which could happen in nuclear power plants. We develop context conditions and antecedent-consequent links of influential factors related to communication error. A case study has been conducted to validate the applicability of the proposed methods

  20. Pressure on Cultivated Land in Fengxian County,Jiangsu Province,China

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Based on the introduction of the general situation of research region,pressure index model of cultivated land is adopted according to the data of population,cultivated land area,crop sowing area,grain sowing area,and unit grain yield in the Statistical Yearbook of Fengxian County.The change of relevant factors of cultivated land pressure index over time is analyzed,as well as the tension level of cultivated land resources.Research shows that the pressure on cultivated land is relatively great in Fengxian County in the years 1999-2005.Grain supply and demand is still under the unsafe state.There will be more prominent contradiction between supply and demand of cultivated land in Fengxian County in the future,and the grain security will under greater pressure.Several countermeasures are put forward to ease the pressure on cultivated land in Fengxian County,in order to realize the sustainable use of cultivated land resources,to ensure the supply and demand balance of grain and cultivated land,and to stabilize and improve the grain productivity in Fengxian County,such as protecting the current cultivated land resources by the strictest protection system of cultivated land,enhancing the intensity of land development and reclamation,enlarging the area of cultivated land,increasing the agricultural sci-tech input,and improving the grain unit yield.

  1. 特色专业建设培养高素质创新型采矿工程专业人才%Cultivate High .Quality Creative Mining Engineering Talents Based on the Construction of Professional Characteristics

    Institute of Scientific and Technical Information of China (English)

    康志强

    2012-01-01

    合理确定采矿工程特色专业建设目标,是采矿工程专业建设首先必须解决的问题。创新人才的培养是采矿工程学科得以存在和发展的关键,对于采矿工程学科具有重要的意义。为了培养出具有采矿特色的"基础实、知识面宽、实践能力强、综合素质高"的高素质创新型采矿工程专业人才,从完善人才培养方案,加强师资队伍建设,改革教学内容及课程体系,提高实践教学质量等方面,阐述了以采矿工程特色专业建设点的建设为依托,进一步完善高素质创新型人才培养体系的方法和措施,从而使学生的综合素质和创新能力得到较大程度的提高。%First of all, mining engineering construction must rationally determine the characteristics of professional development goals of mining engineering. Innovative talents training is the key of existence and development of safe- ty engineering discipline, which is of great significance for mining engineering discipline. In order to train with the characteristics of mining engineering, "based on fact, know/edge breadth, practical ability, and comprehensive high quality" high-quality creative mining engineering talents. The paper based on the construction of mining engineering professional characteristics development points from perfecting training plan strengthening the teaching staff, teach- ing contents and curriculum reform, improvement of education quality, relying on further improving the methods and measures of cultivating the high-quality creative talents. So that the students overall quality and level of creative has been greatly improved.

  2. A Novel Method For Speech Segmentation Based On Speakers' Characteristics

    CERN Document Server

    Abdolali, Behrouz; 10.5121/sipij.2012.3205

    2012-01-01

    Speech Segmentation is the process change point detection for partitioning an input audio stream into regions each of which corresponds to only one audio source or one speaker. One application of this system is in Speaker Diarization systems. There are several methods for speaker segmentation; however, most of the Speaker Diarization Systems use BIC-based Segmentation methods. The main goal of this paper is to propose a new method for speaker segmentation with higher speed than the current methods - e.g. BIC - and acceptable accuracy. Our proposed method is based on the pitch frequency of the speech. The accuracy of this method is similar to the accuracy of common speaker segmentation methods. However, its computation cost is much less than theirs. We show that our method is about 2.4 times faster than the BIC-based method, while the average accuracy of pitch-based method is slightly higher than that of the BIC-based method.

  3. Mass Cultivation of Entomopathogenic Nematode in Artificial Media

    OpenAIRE

    Dyah Rini Indriyanti; Nur Lailatul Muharromah

    2016-01-01

    Entomopathogenic nematodes Entomopathogenic nematodes (EPN) of the genera Heterorhabditis and Steinernema are commercially used to control pest insect. EPN is widely cultivated through in-vivo and in vitro methods. This research aims to discover the abundance of EPN cultivated in various artificial media. Seven types of media composition were tested in this research: media A (yeast + soybean powder), media B (yeast + chicken liver), media C (yeast + dog food), media D (yolk + soybean powder),...

  4. Trinocular Calibration Method Based on Binocular Calibration

    OpenAIRE

    CAO Dan-Dan; Luo, Chun; GAO Shu-Yuan; Wang, Yun; Li, Wen-Bin; XU Zhen-Ying

    2012-01-01

    In order to solve the self-occlusion problem in plane-based multi-camera calibration system and expand the measurement range, a tri-camera vision system based on binocular calibration is proposed. The three cameras are grouped into two pairs, while the public camera is taken as the reference to build the global coordinate. By calibration of the measured absolute distance and the true absolute distance, global calibration is realized. The MRE (mean relative error) of the global calibration of ...

  5. 案例教学法在医德修养教学中的应用%Application of Case Teaching Method in the Cultivation of Medical Ethics

    Institute of Scientific and Technical Information of China (English)

    李东霞

    2015-01-01

    Case teaching methodology is an instructive, practical, lfexible and interesting approach. It is suitable for application in the medical ethics education program. There are still some problems in case teaching, such as inadequate understanding of teaching methods, improperly selected cases and implementation method which does not reach the goal, etc. Methods solving these problems include deepened understanding of case teaching, scientifically selected cases, vividly demonstration, generalization and summarization. Case teaching methodology is a new and suitable approach for the medical ethics education.%案例教学法具有启发性、实践性、灵活性和趣味性等特点,适合应用于医德修养教学中。目前案例教学中还存在一些问题,如教师对案例教学的认识不足,案例选择不当,实施方法不到位等。解决的对策可以有加深教师对案例教学的认识,科学筛选案例,生动展现和归纳总结等方法。案例教学是适合医德教育特点的一种全新的教学方法。

  6. A Study and Practice of College Students' Emotional Quotient Cultivation Based on Quality Development%基于素质拓展平台大学生情商培养研究与实践

    Institute of Scientific and Technical Information of China (English)

    嵇敏; 杨丹; 李冠华; 张玲彬

    2015-01-01

    基于素质拓展平台新模式运作下大学生情商培养研究与实践,通过在河海大学大禹学院2013级本科生中开展野外素质拓展,寒暑假感恩教育,社会实践、公益劳动等素质拓展学分的系列活动,在实践的强化培养下,增强学生的团结、爱心、宽容、感恩、自信等情商特性,以促进学生更好地认识自我、管理自我、激励自我。%This paper is a study and practice of college students' Emotional Quotient cultivation based on a new model which is quality development. In Grade 2013 undergraduates in Dayu College of Hohai University, field quality development, thanks-giving education during summer and winter vacation, social prac-tice, welfare work and other activities of quality development credits were held. Under the practice of intensive cultivation, students' non-intelligence characteristics of EQ, such as solidar-ity, love, tolerance, thanksgiving and self-confidence, , were strengthened, and they could understand, manage themselves, and motivate themselves better.

  7. Evaluation on Cultivated Land Resource Ecologi-cal Service Value in Wuhan Metropolitan Area

    Institute of Scientific and Technical Information of China (English)

    Jinhua TANG; Yinghui Ll; Gangqiang CHEN; Lei YU; Sujuan Ll

    2014-01-01

    The land ecosystem service function value contains the gas regulating function value, air purification function value, water conservation value, land conser-vation value, soil nutrient conservation value and maintain biodiversity value. Based on the method of market value and the data of Hubei Statistical yearbook, cultivat-ed land resources of ecosystem service function value is 730 038.69Mil ion in 2007-2009, and 714 774.99 Mil ion in 2010-2012 in Wuhan Metropolitan Area. After three years of development, Wuhan Metropolitan Area of ecological value of cultivated land has increased by about 3.97%. From the point of each composition, the im-portance of gas regulating function of cultivated land resources and water conserva-tion function is the forefront. From the point of spatial distribution, the highest of annual average value of the cultivated land ecological was Huanggang (1.725 bil ion yuan), the lowest was Ezhou (217.868 6 mil ion yuan) in 2010-2012. From the point of space changes, the land ecological value increased only in Qianjiang city, while farmland ecological value in other cities was fal ing The conclusion is of direc-tive significance to the regional land protection policy and the farmland reasonable distribution, for Wuhan Metropolitan Area and other areas.

  8. Utilization of industrial dairy waste as microalgae cultivation medium : a potential study for sustainable energy resources

    Science.gov (United States)

    Nurmayani, S.; Sugiarti, Y.; Putra, R. H.

    2016-04-01

    Microalgae is one of biodiesel resources and call as third generation biofuel. Biodiesel is one alternative energy that being developed. So study about resource of biodiesel need a development, for the example is development the basic material such as microalgae. In this paper we explain the potential use of dairy waste from industry as a cultivation medium of microalgae for biodiesel production. Dairy waste from dairy industry contains 34.98% protein, 4.42% lactose, 9.77% fiber, 11.04% fat, 2.33% calcium, 1.05% phosfor, and 0.4 % magnesium, meaning that the dairy waste from dairy industry has a relatively high nutrient content and complete from a source of carbon, nitrogen and phosphorus as macro nutrients. The method in this paper is literature review to resulting a new conclusion about the potency of waste water from dairy industry as microalgae cultivation medium. Based on the study, the dairy waste from dairy industry has potency to be used as cultivation medium of Botryococcus braunii in the production of biodiesel, replacing the conventional cultivation medium.

  9. Effects of Urbanization-Induced Cultivated Land Loss on Ecosystem Services in the North China Plain

    Directory of Open Access Journals (Sweden)

    Wei Song

    2015-06-01

    Full Text Available Since the implementation of market oriented economic reform in 1978, China has been on the track of rapid urbanization. The unprecedented urbanization in China has resulted in substantial cultivated land loss and rapid expansion of urban areas. The cultivated land loss due to urbanization not only threatens food security in China, but has also led to ecological system degradation to which close attention should be paid. Therefore, we examined the effects of the conversion from cultivated to urban areas on the ecosystem service in the North China Plain on the basis of a net primary productivity based ecosystem service model (NESM and a buffer comparison method. Cultivated land loss due to urbanization in the North China Plain led to a total loss of ecosystem service value of 34.66% during the period 1988–2008. Urban expansion significantly decreased the ecosystem service function of water conservation (–124.03%, nutrient cycling (–31.91%, gas regulation (−7.18%, and organic production (–7.18%, while it improved the soil conservation function (2.40%. Land use change accounted for 57.40% of the changes in ecosystem service and had a major influence on the changes in nutrient cycling and water conservation. However, climate change mainly determined the changes in gas regulation, organic production, and soil conservation.

  10. AN EVEN COMPONENT BASED FACE RECOGNITION METHOD

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    This paper presents a novel face recognition algorithm. To provide additional variations to training data set, even-odd decomposition is adopted, and only the even components (half-even face images) are used for further processing. To tackle with shift-variant problem,Fourier transform is applied to half-even face images. To reduce the dimension of an image,PCA (Principle Component Analysis) features are extracted from the amplitude spectrum of half-even face images. Finally, nearest neighbor classifier is employed for the task of classification. Experimental results on ORL database show that the proposed method outperforms in terms of accuracy the conventional eigenface method which applies PCA on original images and the eigenface method which uses both the original images and their mirror images as training set.

  11. LEVEL SET METHODS BASED ON DISTANCE FUNCTION

    Institute of Scientific and Technical Information of China (English)

    王德军; 唐云; 于洪川; 唐泽圣

    2003-01-01

    Some basic problems on the level set methods were discussed, such as the method used to preserve the distance function, the existence and uniqueness of solution for the level set equations. The main contribution is to prove that in a neighborhood of the initial zero level set, the level set equations with the restriction of the distance function have a unique solution, which must be the signed distance function with respect to the evolving surface. Some skillful approaches were used: Noticing that any solution for the original equation was a distance function, the original level set equations were transformed into a simpler alternative form. Moreover, since the new system was not a classical one, the system was transforned into an ordinary one, for which the implicit function method was adopted.

  12. Valuing Convertible Bonds Based on LSRQM Method

    Directory of Open Access Journals (Sweden)

    Jian Liu

    2014-01-01

    Full Text Available Convertible bonds are one of the essential financial products for corporate finance, while the pricing theory is the key problem to the theoretical research of convertible bonds. This paper demonstrates how to price convertible bonds with call and put provisions using Least-Squares Randomized Quasi-Monte Carlo (LSRQM method. We consider the financial market with stochastic interest rates and credit risk and present a detailed description on calculating steps of convertible bonds value. The empirical results show that the model fits well the market prices of convertible bonds in China’s market and the LSRQM method is effective.

  13. Environmental life cycle assessment of Ethiopian rose cultivation.

    Science.gov (United States)

    Sahle, Abiy; Potting, José

    2013-01-15

    A life cycle assessment (LCA) was conducted for Ethiopian rose cultivation. The LCA covered the cradle-to-gate production of all inputs to Ethiopian rose cultivation up to, and including transport to the Ethiopian airport. Primary data were collected about materials and resources used as inputs to, and about the product outputs from 21 farms in 4 geographical regions (i.e. Holleta, Sebeta, Debre Ziet, and Ziway). The primary data were imported in, and analyzed with the SimaPro7.3 software. Data for the production of used inputs were taken from the EcoInvent®2.0 database. Emissions from input use on the farms were quantified based on estimates and emission factors from various studies and guidelines. The resulting life cycle inventory (LCI) table was next evaluated with the CML 2 baseline 2000 V2/world, 1990/characterization method to quantify the contribution of the rose cultivation chain to 10 environmental impact categories. The set of collected primary data was comprehensive and of high quality. The data point to an intensive use of fertilizers, pesticides, and greenhouse plastic. Production and use of these inputs also represent the major contributors in all environmental impact categories. The largest contribution comes from the production of the used fertilizers, specifically nitrogen-based fertilizers. The use of calcium nitrate dominates Abiotic Depletion (AD), Global Warming (GW), Human Toxicity (HT) and Marine Aquatic Ecotoxicity (MAET). It also makes a large contribution to Ozone Depletion (OD), Acidification (AD) and Fresh water Aquatic Ecotoxicity (FAET). Acidification (AC) and Eutrophication (EU) are dominated by the emission of fertilizers. The emissions from the use of pesticides, especially insecticides dominate Terrestrial Ecotoxicity (TE) and make a considerable contribution to Freshwater Aquatic Ecotoxicity (FAET) and Photochemical Oxidation (PhO). There is no visible contribution from the use of pesticides to the other toxicity categories

  14. HMM-Based Gene Annotation Methods

    Energy Technology Data Exchange (ETDEWEB)

    Haussler, David; Hughey, Richard; Karplus, Keven

    1999-09-20

    Development of new statistical methods and computational tools to identify genes in human genomic DNA, and to provide clues to their functions by identifying features such as transcription factor binding sites, tissue, specific expression and splicing patterns, and remove homologies at the protein level with genes of known function.

  15. String-Based Methods in Perturbative Gravity

    OpenAIRE

    Bern, Z.; Dunbar, D.C.; Shimada, T.

    1993-01-01

    String theory implies a relatively modest growth in computational complexity for perturbative gravity calculations as compared to gauge theory calculations, contrary to field theory expectations. An explicit string-based calculation, which would be extremely difficult using conventional techniques, is presented to illustrate this.

  16. Utilization zoning of cultivated land based on net primary productivity in Guanzhong-Tianshui Economic Region%基于净初级生产力的关中-天水经济区耕地利用分区研究

    Institute of Scientific and Technical Information of China (English)

    杨海娟; 温晓金; 刘焱序

    2013-01-01

    As a populous nation, improve grain production capacity along with rational use and protection of cultivated land resources has posed a considerable challenge in domestic agriculture and land related research in China. Higher NPP for cultivated lands has suggested the existence of more organic biomass. This has been critical for the final production of food crops in the country. It was therefore likely for research on NPP to provide the basis for resolving food security issues. Functional zoning has been the commonly used method to guarantee sustainable use of land. Presently, however, heavily fragmented research merely described real supply of cultivated lands. A deeper understand on the potential reserves of cultivated lands was needed in this regard. Based on remote sensing observation, it is possible to have statistics of the output of a large number of cultivated lands within a short time. Compared with the yearbook data, remote sensing observation has advantages including timeliness and spatial precision. Remote sensing observations have therefore been strongly supplemental to statistical data. NPP estimated by remote sensing was used as crop biomass in cultivated lands instead of the traditional calculations based statistics data. Cultivated land in the Guanzhong-Tianshui Economic Region (GTER) was zoned by using neural network algorithm model and remote sensing data in 2001-2009 substituting for statistic crop yield data. Then the wavelet neural network was used to predict the NPP in the zoned regions. Three results were eventually attained. 1) From 2002 to 2009, total estimated NPP per year in GTER was 1.6×107 t. It showed large variation patterns between estimated NPP data and statistics grain data for cultivated lands in GTER. This suggested statistical and remote sensing data were not substitutable for one another. As clustering function was unknown, zoning via estimated NPP data reflected a more universal adaptability than via statistical data. 2) The

  17. Independent Awareness Cultivation of Infants Based on Individual Psychology%基于个性心理的婴幼儿独立意识培养

    Institute of Scientific and Technical Information of China (English)

    关金艳

    2013-01-01

      独立意识是评价人体心理健康成熟与否的重要标准。在婴幼儿教育领域,独立意识是婴幼儿心理健康成熟和心理健康发展的重要指标。通过独立意识的培养能够促进婴幼儿运动能力、语言能力、思维能力、智力,以及社会适应能力的全面发展。目前国内外学者对婴幼儿独立意识的研究主要是从年龄、生理过程、独立性特性,以及教学方面进行的,缺乏从心理学专业角度分析其成因、干预对策和效果。为培养婴幼儿的独立意识,首先要营造常态化的和谐的家庭氛围,其次要打造稳定的心理教学队伍,再次要通过优秀的动画片等向婴幼儿传递独立意识。%Independent awareness is an important standard to evaluate whether a person is mature in mental mealth .In the field of infant education ,independent awareness is an important index of the mental maturi‐ty and mental health development of the infants .The cultivation of independent awareness can promote the all‐round development of infants’ ability to do sports ,to speak ,to think and their intelligence and their social adaptability .At present foreign scholars study the independent awareness of infants mainly from such aspects as age ,physiological process ,independence adaptability and teaching ,which lacks profession‐al analysis of its causes ,intervention countermeasures and effects from psychological perspective .In order to cultivate the infants’ independent consciousness ,we must create a normal and harmonious family at‐mosphere in the first place .Secondly ,we should foster stable teaching staff of psychology .Finally we should teach the infants independent awareness through excellent cartoons .

  18. Bionic optimization research of soil cultivating component design

    Institute of Scientific and Technical Information of China (English)

    GUO ZhiJun; ZHOU ZhiLi; ZHANG Yi; LI ZhongLi

    2009-01-01

    The basic biomechanical laws that apply to the clawed toes of animals with powerful digging abilities and the optimal bionic design of curved soil cultivating components with an analogous contour were researched in a novel way. First, the curvature and profile of the inside contour line of a field mouse's clawed toe were analyzed. The finite element method (FEM) was then used to simulate the working process in order to study the changing characteristics of the working resistance of bionic soil-engaging surfaces and the stress field of the processed soil. A straight-line cultivating component was used for comparative analysis. In accordance with the simulation results, a series of soil cultivating components of varying design were manufactured. An indoor soil bin experiment was carried out to measure their working resistance and validate the results of the FEM analysis. The results of this research would have important values in the optimization design of cultivating components for energy and cost savings.

  19. Valuing Convertible Bonds Based on LSRQM Method

    OpenAIRE

    Jian Liu; Lizhao Yan; Chaoqun Ma

    2014-01-01

    Convertible bonds are one of the essential financial products for corporate finance, while the pricing theory is the key problem to the theoretical research of convertible bonds. This paper demonstrates how to price convertible bonds with call and put provisions using Least-Squares Randomized Quasi-Monte Carlo (LSRQM) method. We consider the financial market with stochastic interest rates and credit risk and present a detailed description on calculating steps of convertible bonds value. The e...

  20. An ICA based method for texture recognition

    OpenAIRE

    Fournel, Thierry; Becker, Jean-Marie; Daniela COLTUC; Yann BOUTANT

    2006-01-01

    The method proposed in this paper uses the Independent Component Analysis (ICA) for an application of unsupervised recognition of textures. The analysed texture is modelled by a weighted sum of almost statistically independent random signals that are extracted with FastICA algorithm. Each resulting signal is described by its negentropy, more precisely, by one of the approximations used by FastICA algorithm. The approximated negentropies are sorted into descending order and represented by a cu...

  1. Nitrous oxide emissions from rapeseed cultivation in Germany

    Science.gov (United States)

    Fuß, Roland; Andres, Monique; Hegewald, Hannes; Kesenheimer, Katharina; Koebke, Sarah; Räbiger, Thomas; Suárez Quiñones, Teresa; Walter, Katja; Stichnothe, Heinz; Flessa, Heinz

    2016-04-01

    About 12 % of Germany's agricultural area is used for rapeseed cultivation and two third of the harvest is converted to biodiesel. Due to requirements of the EU Renewables Directive the greenhouse gas (GHG) balance of rapeseed cultivation must be reported and sustainability criteria and GHG savings compared to fossil fuel must be achieved and certified. Current certified methodology estimates N2O field emissions from rapeseed cultivation using the IPCC Tier 1 approach based on a global emission factor (N2O emission per unit nitrogen fertilizer input) of 1 %, which is not specific for the crop. We present results from three years of measurements (2013 - 2015) on five field trials in Germany, which combined with data from a meta-analysis suggest that GHG emission factors of German rapeseed cultivation are lower than thought previously. Furthermore, results suggest that substitution of mineral fertilizers with organic fertilizers is a valid mitigation option since it avoids GHG emissions during production of mineral fertilizers.

  2. Genotypic diversity among rhizospheric bacteria of three legumes assessed by cultivation-dependent and cultivation-independent techniques.

    Science.gov (United States)

    Pongsilp, Neelawan; Nimnoi, Pongrawee; Lumyong, Saisamorn

    2012-02-01

    The genotypic diversity of rhizospheric bacteria of 3 legumes including Vigna radiata, Arachis hypogaea and Acacia mangium was compared by using cultivation-dependent and cultivation-independent methods. For cultivation-dependent method, Random amplified polymorphic DNA (RAPD) profiles revealed that the bacterial genetic diversity of V. radiata and A. mangium rhizospheres was higher than that of A. hypogaea rhizosphere. For cultivation-independent method, Denaturing gradient gel electrophoresis (DGGE) profiles of PCR-amplified 16S rRNA genes revealed the difference in bacterial community and diversity of rhizospheres collected from 3 legumes. The ribotype richness which indicates species diversity, was highest in V. radiata rhizosphere, followed by A. hypogaea and A. mangium rhizospheres, respectively. Three kinds of media were used to cultivate different target groups of bacteria. The result indicates that the communities of cultivable bacteria in 3 rhizospheres recovered from nutrient agar (NA) medium were mostly different from each other, while Bradyrhizobium selective medium (BJSM) and nitrogen-free medium shaped the communities of cultivable bacteria. Nine isolates grown on BJSM were identified by 16S rRNA gene sequence analysis. These isolates were very closely related (with 96% to 99% identities) to either one of the three groups including Cupriavidus-Ralstonia group, Bacillus group and Bradyrhizobium-Bosea-Afipia group. The rhizospheres were also examined for their enzymatic patterns. Of 19 enzymes tested, 3 rhizospheres were distinguishable by the presence or the absence of leucine acrylamidase and acid phosphatase. The selected cultivable bacteria recovered from NA varied in their abilities to produce indole-acetic acid and ammnonia. The resistance to 10 antibiotics was indistinguishable among bacteria isolated from different rhizospheres. PMID:22806857

  3. The use of 32P Method to Evaluate the Growth of Lowland Rice Cultivated in a System of Rice Intensification (SRI

    Directory of Open Access Journals (Sweden)

    A. Citraresmini

    2013-08-01

    Full Text Available A pot experiment has been conducted to evaluate the growth of the Dyah Suci, a lowland rice variety, in an SRI (System of Rice Intensification planting system. The phosphorus-32 (32P isotope technique was used to evaluate the growth of plants in relation with their phosphorus uptake. The uptake was assumed to vary in the same direction as the growth of the plant. The 32P uptake is assumed to vary in the opposite direction to the plant’s total phosphorus uptake. Here the 32P uptake is expressed in count per minutes (cpm which is then transformed to disintegration per minute (dpm. The results show that, in terms of promoting the plant’s uptake of phosphorus, the SRI planting system is superior to the conventional planting system, and it is manifested in the higher dry weight of straw and grain. From this experiment it is concluded that the 32P method can be used satisfactorily as a tool for explaining the relation between P-uptake and plant growth

  4. 基于形象思维的创新能力培养的分析%Analysis of Cultivation for Innovation Ability Based on Thinking in Images

    Institute of Scientific and Technical Information of China (English)

    赵晓运

    2012-01-01

    分析了创新教育中形象思维的独特性、基础性和源头性,指出创新能力是一种社会属性,可以通过锻炼和培养而得到提高.首次提出了结构功能原理“使用情景”的形象认识.通过对教学实践和创新应用实践的分析,强调了训练和触发形象思维能力,可以有效地、显著地提高创新思维能力.%Uniqueness, foundation and fountainhead of thinking in images in innovation education are analyzed. Innovation ability is one type of social attributes and can be improved through practice and cultivation. Imaginal recognition of usage scenario to structural function principles is initially proposed. Practice and triggering of imaginal thinking ability, which can effectively and markedly enhance innovation thinking ability, are emphasized under the analysis of teaching practices and innovation applied practices.

  5. Knowledge-based methods for control systems

    International Nuclear Information System (INIS)

    This thesis consists of three projects which combine artificial intelligence and control. The first part describes an expert system interface for system identification, using the interactive identification program Idpac. The interface works as an intelligent help system, using the command spy strategy. It contains a multitude of help system ideas. The concept of scripts is introduced as a data structure used to describe the procedural part of the knowledge in the interface. Production rules are used to represent diagnostic knowledge. A small knowledge database of scripts and rules has been developed and an example run is shown. The second part describes an expert system for frequency response analysis. This is one of the oldest and most widely used methods to determine the dynamics of a stable linear system. Though quite simple, it requires knowledge and experience of the user, in order to produce reliable results. The expert system is designed to help the user in performing the analysis. It checks whether the system is linear, finds the frequency and amplitude ranges, verifies the results, and, if errors should occur, tries to give explanation and remedies for them. The third part describes three diagnostic methods for use with industrial processes. They are measurement validation, i.e., consistency checking of sensor and measurement values using any redundancy of instrumentation; alarm analysis, i.e. analysis of multiple alarm situations to find which alarms are directly connected to primary faults and which alarms are consequential effects of the primary ones; and fault diagnosis, i.e., a search for the causes of and remedies for faults. The three methods use multilevel flow models, (MFM), to describe the target process. They have been implemented in the programming tool G2, and successfully tested on two small processes. (164 refs.) (au)

  6. Scope-Based Method Cache Analysis

    DEFF Research Database (Denmark)

    Huber, Benedikt; Hepp, Stefan; Schoeberl, Martin

    requests memory transfers at well-defined instructions only. In this article, we present a new cache analysis framework that generalizes and improves work on cache persistence analysis. The analysis demonstrates that a global view on the cache behavior permits the precise analyses of caches which are hard......The quest for time-predictable systems has led to the exploration of new hardware architectures that simplify analysis and reasoning in the temporal domain, while still providing competitive performance. For the instruction memory, the method cache is a conceptually attractive solution, as it...

  7. Proposal for Scrambled Method based on NTRU

    Directory of Open Access Journals (Sweden)

    Ahmed Tariq Sadiq

    2015-08-01

    Full Text Available Scrambling is widely used to protect the security of data files such as text, image, video or audio files; however, it is not the most efficient method to protect the security of the data files. This article uses NTRU public key cryptosystem to increase the robustness of scrambling of sound files. In this work, we convert the sound file into text, and then scramble it in the following way: first, we encrypt the header of the sound file then, scramble the data of the file after the header in three stages. In each stage we scramble the data of the sound file and keep the original order of data in an array then, the three arrays are encrypted by the sender and sent with the encrypted header to the receiver in one file, while the scrambled data of the sound file is sent to the receiver in another file. We have tested the proposed method on several sound files; the results show that the time of encryption and decryption is reduced to approximately one-third, or less, compared to encrypting the file using NTRU.

  8. Gradient-based methods for sparse recovery

    CERN Document Server

    Hager, William; Zhang, Hongchao

    2009-01-01

    The convergence rate is analyzed for the SpaSRA algorithm (Sparse Reconstruction by Separable Approximation) for minimizing a sum $f (\\m{x}) + \\psi (\\m{x})$ where $f$ is smooth and $\\psi$ is convex, but possibly nonsmooth. It is shown that if $f$ is convex, then the error in the objective function at iteration $k$, for $k$ sufficiently large, is bounded by $a/(b+k)$ for suitable choices of $a$ and $b$. Moreover, if the objective function is strongly convex, then the convergence is $R$-linear. An improved version of the algorithm based on a cycle version of the BB iteration and an adaptive line search is given. The performance of the algorithm is investigated using applications in the areas of signal processing and image reconstruction.

  9. Triptycene-based dianhydrides, polyimides, methods of making each, and methods of use

    KAUST Repository

    Ghanem, Bader

    2015-12-30

    A triptycene-based monomer, a method of making a triptycene-based monomer, a triptycene-based aromatic polyimide, a method of making a triptycene- based aromatic polyimide, methods of using triptycene-based aromatic polyimides, structures incorporating triptycene-based aromatic polyimides, and methods of gas separation are provided. Embodiments of the triptycene-based monomers and triptycene-based aromatic polyimides have high permeabilities and excellent selectivities. Embodiments of the triptycene-based aromatic polyimides have one or more of the following characteristics: intrinsic microporosity, good thermal stability, and enhanced solubility. In an exemplary embodiment, the triptycene-based aromatic polyimides are microporous and have a high BET surface area. In an exemplary embodiment, the triptycene-based aromatic polyimides can be used to form a gas separation membrane.

  10. Study on Stratification Cultivation of Automobile after-sale Service Talents in Higher Vocational Colleges Based on AHP%基于AHP法的高职汽车售后服务人才分层培养研究

    Institute of Scientific and Technical Information of China (English)

    鲍婷婷

    2014-01-01

    汽车售后服务人才是影响汽车维修企业发展的核心要素。运用AHP法对汽车售后服务人才所需要的能力进行分析,从专业技能、社会技能和概念技能三个方面构建人才评价体系,并提出了相应的分层培养策略。%Automobile after-sale service talents are the key factor of the development of automobile repair enterprises. This paper an⁃alyzes the abilities required by automobile after-sale service talents based on AHP. The talents evaluating system is constructed from the three aspects of professional skills, social skills and conceptual skills, and corresponding tactics for stratification cultivation are put forward.

  11. Forest cover dynamics of shifting cultivation in the Democratic Republic of Congo: a remote sensing-based assessment for 2000-2010

    Science.gov (United States)

    Molinario, G.; Hansen, M. C.; Potapov, P. V.

    2015-09-01

    Shifting cultivation has traditionally been practiced in the Democratic Republic of Congo by carving agricultural fields out of primary and secondary forest, resulting in the rural complex: a characteristic land cover mosaic of roads, villages, active and fallow fields and secondary forest. Forest clearing has varying impacts depending on where it occurs relative to this area: whether inside it, along its primary forest interface, or in more isolated primary forest areas. The spatial contextualization of forest cover loss is therefore necessary to understand its impacts and plan its management. We characterized forest clearing using spatial models in a Geographical Information System, applying morphological image processing to the Forets d’Afrique Central Evaluee par Teledetection product. This process allowed us to create forest fragmentation maps for 2000, 2005 and 2010, classifying previously homogenous primary forest into separate patch, edge, perforated, fragmented and core forest subtypes. Subsequently we used spatial rules to map the established rural complex separately from isolated forest perforations, tracking the growth of these areas in time. Results confirm that the expansion of the rural complex and forest perforations has high variance throughout the country, with consequent differences in local impacts on forest ecology and habitat fragmentation. Between 2000 and 2010 the rural complex grew by 10.2% (46 182 ha), increasing from 11.9% to 13.1% of the total land area (1.2% change) while perforated forest grew by 74.4% (23 856 ha), from 0.8% to 1.5%. Core forest decreased by 3.8% (54 852 ha), from 38% to 36.6% of the 2010 land area. Of particular concern is the nearly doubling of perforated forest, a land dynamic that represents greater spatial intrusion of forest clearing within core forest areas and a move away from the established rural complex.

  12. Iterative Methods for the Force-based Quasicontinuum Approximation

    OpenAIRE

    Dobson, Matthew; Luskin, Mitchell; Ortner, Christoph

    2009-01-01

    Force-based atomistic-continuum hybrid methods are the only known pointwise consistent methods for coupling a general atomistic model to a finite element continuum model. For this reason, and due to their algorithmic simplicity, force-based coupling methods have become a popular class of atomistic-continuum hybrid models as well as other types of multiphysics models. However, the recently discovered unusual stability properties of the linearized force-based quasicontinuum (QCF) approximation,...

  13. A network diagnostics method based on pattern recognition algorithms

    OpenAIRE

    Olizarovich, E. V.; Rodchenko, V. G.

    2009-01-01

    This report deals with the problem of designing and building of a computer network diagnostic system. Diagnostic content problems are reviewed, as well as ways of their solution based on mathematical and computer modelling methods. A traffic analysis-based diagnostic method is suggested for process statuses in a computer network. The method is based on algorithms of the mathematical pattern recognition theory. To build a diagnostic system, a multi-level model building and verification arrange...

  14. Statistical Analysis of the Cultivating Methods of Construction Workers' Safe Behavior Habits%建筑工人安全行为习惯培养方法的统计分析

    Institute of Scientific and Technical Information of China (English)

    杨高升; 居婕

    2013-01-01

    建筑工人的不安全行为直接导致了安全事故的发生,从行为心理学的角度分析了建筑工人不安全行为形成机理,并从中找出影响因素.细分安全行为习惯的培养方法:正强化、负强化、惩罚、教育培训,并通过对南京市两家建筑单位的管理人员进行问卷抽样调查,利用SPSS软件对安全行为习惯培养的四种方法进行统计分析,其中安全教育培训最为重要,结合国外安全培训模式经验,提出可行性的措施.%The construction workers unsafe behavior is the direct cause of security incidents. Analyzes the formation mechanism of construction workers' unsafe behavior from the perspective of behavior psychology, and finds out the factors. Subdivides the cultivating methods of safe behavior habits into four sectors, namely, positive reinforcement, negative reinforcement, punishment, and training. Through the questionnaire survey on the management of the two building units in Nanjing and using the SPSS software to conduct the statistical analysis of the four methods of fostering safe behavior habits, this paper finds that the safe education and training are the most important factors to foster safe behavior habits. At last, this paper gives the feasible measures combined with foreign experience in safety training mode.

  15. Genetic structure in cultivated and wild carrots (¤Daucus carota¤ L.) revealed by AFLP analysis

    DEFF Research Database (Denmark)

    Shim, S.I.; Bagger Jørgensen, Rikke

    2000-01-01

    Genetic variation within and among five Danish populations of wild carrot and five cultivated varieties was investigated using amplified fragment length polymorphism (AFLP). Ten AFLP primer combinations produced 116 polymorphic bands. Based on the marker data an UPGMA-cluster analysis and principal...... markers specific to the cultivated carrot makes it possible to detect introgression from cultivated to wild types....

  16. Dynamics of decadal changes in the distribution of double-cropping rice cultivation in China

    Institute of Scientific and Technical Information of China (English)

    DUAN JuQi; ZHOU GuangSheng

    2013-01-01

    Quantitative description of changes in the distribution of paddy rice cultivation in response to recent climate change provides a reference for rice cultivation patterns and formulation of countermeasures to cope with future climate change in China.This study analyzes the dynamics of decadal changes in distribution of double-cropping rice in China during 1961-2010 in relation to climate change based on the maximum entropy method.Decadal changes in the double-cropping rice cultivation area and climatic suitability in China were apparent.The total area of climatically suitable regions was highest in the 1960s,and subsequently showed an increasing trend at first and then a decreasing trend from the 1970s to 2000s.However,the low climatic suitability area decreased,which implied that the moderate and high climatic suitability areas increased.Among the latter,the high climatic suitability area showed the highest increase in extent to 4.4 times that of the 1990s and four times that of the 1960s.The areas of double-cropping rice cultivation most sensitive to climate change are mainly located in central Jiangsu,central Anhui,the eastern Sichuan Basin,southern Henan and central Guizhou.Transformation of areas between low and moderate climatic suitability was observed in northern Zhejiang,southern Anhui and Hubei,and northern Guangxi.Transformation of areas between moderate and high climatic suitability was observed in central Jiangxi and Leizhou Peninsula.The northern boundary of double-cropping rice cultivation in China shifted southwards and contracted eastwards in the 1970s,and extended northwards in the 1980s.However,the northern boundary did not shift northwards in response to climate warming in the 2000s.

  17. Answer Card Identification Method Based on Pattern Recognition Technology

    OpenAIRE

    Xu Jing; Zhang Kun Fang

    2016-01-01

    The traditional answer card reading method using OMR (Optical Mark Reader), most commonly, OMR special card special use, less versatile, high cost, aiming at the existing problems proposed a method based on pattern recognition of the answer card identification method. Using the method based on Line Segment Detector to detect the tilt of the image, the existence of tilt image rotation correction, and eventually achieve positioning and detection of answers to the answer sheet .Pattern recogniti...

  18. Brain Based Teaching: Fad or Promising Teaching Method.

    Science.gov (United States)

    Winters, Clyde A.

    This paper discusses brain-based teaching and examines its relevance as a teaching method and knowledge base. Brain-based teaching is very popular among early childhood educators. Positive attributes of brain-based education include student engagement and active involvement in their own learning, teachers teaching for meaning and understanding,…

  19. Development of methodology for the fore cast of microbiological processes under transaction to industrial cultivation

    International Nuclear Information System (INIS)

    Proposals for possible cooperation with Western partners : To obtain the scale transfers method in laboratory condition of microorganisms cultivation to industrial conditions based on the parameters of spatial cultivation to industrial conditions based on the parameters of spatial heterogeneous hydrodynamics situation in bioreactors. The problem is the impossibility to count constructive elements and regimes of ferments operation which provided optimum environment for microorganisms vital functions because the hydrodynamic, biological and mass change processes are complicated. To solve the problems it is required to : - Investigate the different sides of physiology of culture-producer of Biologically Active Substances (hereinafter BAS) - Investigate the interrelation between the stirring and biological transformation in microorganism cells - Analyze and search main tendencies required to control biosynthesis (BAS) processes and reproduction of biosynthesis results at the cultivation change scale - Analyze technical properties of the reactor and the revealing of the spatial heterogeneous hydrodynamics situation at the different scales of bioreactor parameters - Investigate cinematic energy mediums field in the different bioreactor scales - Obtain the criteria dependencies estimating the irregularity of the stirrings intensity - Prepare the methodological foundations of microbiological processes forecast required to introduce to the industrial biosynthesis environment Expected results : To detect the comparable regimes of bioreactor operation in order to achieve equal production range and realize the scale-up method

  20. Impacts of recent cultivation on genetic diversity pattern of a medicinal plant, Scutellaria baicalensis (Lamiaceae

    Directory of Open Access Journals (Sweden)

    Shao Ai-Juan

    2010-04-01

    Full Text Available Abstract Background Cultivation of medicinal plants is not only a means for meeting current and future demands for large volume production of plant-based drug and herbal remedies, but also a means of relieving harvest pressure on wild populations. Scutellaria baicalensis Georgi (Huang-qin or Chinese skullcap is a very important medicinal plant in China. Over the past several decades, wild resource of this species has suffered rapid declines and large-scale cultivation was initiated to meet the increasing demand for its root. However, the genetic impacts of recent cultivation on S. baicalensis have never been evaluated. In this study, the genetic diversity and genetic structure of 28 wild and 22 cultivated populations were estimated using three polymorphic chloroplast fragments. The objectives of this study are to provide baseline data for preserving genetic resource of S. baicalensis and to evaluate the genetic impacts of recent cultivation on medicinal plants, which may be instructive to future cultivation projects of traditional Chinese medicinal plants. Results Thirty-two haplotypes of S. baicalensis (HapA-Y and Hap1-7 were identified when three chloroplast spacers were combined. These haplotypes constituted a shallow gene tree without obvious clusters for cultivated populations, suggesting multiple origins of cultivated S. baicalensis. Cultivated populations (hT = 0.832 maintained comparable genetic variation with wild populations (hT = 0.888, indicating a slight genetic bottleneck due to multiple origins of cultivation. However, a substantial amount of rare alleles (10 out of 25 haplotypes within wild populations lost during the course of S. baicalensis cultivation. The genetic differentiation for cultivated group (GST = 0.220 was significantly lower than that of wild group (GST = 0.701. Isolation by distance analysis showed that the effect of geographical isolation on genetic structure was significant in wild populations (r = 0.4346, P r

  1. AN IMAGE RETRIEVAL METHOD BASED ON SPATIAL DISTRIBUTION OF COLOR

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Color histogram is now widely used in image retrieval. Color histogram-based image retrieval methods are simple and efficient but without considering the spatial distribution information of the color. To overcome the shortcoming of conventional color histogram-based image retrieval methods, an image retrieval method based on Radon Transform (RT) is proposed. In order to reduce the computational complexity,wavelet decomposition is used to compress image data. Firstly, images are decomposed by Mallat algorithm.The low-frequency components are then projected by RT to generate the spatial color feature. Finally the moment feature matrices which are saved along with original images are obtained. Experimental results show that the RT based retrieval is more accurate and efficient than traditional color histogram-based method in case that there are obvious objects in images. Further more, RT based retrieval runs significantly faster than the traditional color histogram methods.

  2. Empirical or rational truffle cultivation? It is time to choose.

    Directory of Open Access Journals (Sweden)

    Gerard Chevalier

    2014-08-01

    Full Text Available Aim of study: The aim of this study was to finalize a new method of truffle cultivation in order to obtain an earlier, more regular and  sizeable production of high quality fruiting bodies. Area of study: The experimentation was carried out in France (country of Dordogne, south-western  France  and Italy (country of  Marches,   eastern central Italy for more than one  decade.Material and methods: For the first time the method is based on scientific data on truffle biology particularly: the dynamic system of mycorrhization by the truffle and by other fungi;  the saprophytic capability of the truffle; the ability of its mycelium for decomposing certain minerals and organic materials in the “brûlés”.The basic principle concern the work of the soil and the upkeep of the root system: to work the soil  immediately after the plantation of mycorrhizal seedlings, deeply enough, with adapted tools,  in order to do not compact the soil,  aerate it,  favour the production of deep fruiting bodies not exposed to high temperatures, dryness, frost, parasites… ;  cut accurately  the roots in order to regenerate them and consequently to provide food for the truffle mycelium.Main results: The result has been a new cultivation method designated “differentiated” and called “MRT”, with adapted work of the soil on the lines of plantation and upkeep of the grass between them,  to maintain the cohabitation between areas where the mycelium is present, from those where it is absent.Research highlights: Research is going on to improve   the techniques and particularly finalize tools more precise and more adapted for working the soil and maintaining  the root system in a  best way.  Keywords: truffle; production; nutrition; soil, root system; cultivation tools. 

  3. A facile method for the preparation of chitosan-based scaffolds with anisotropic pores for tissue engineering applications.

    Science.gov (United States)

    Ran, Jiabing; Xie, Lingjun; Sun, Guanglin; Hu, Jingxiao; Chen, Si; Jiang, Pei; Shen, Xinyu; Tong, Hua

    2016-11-01

    To date, great efforts have been made to prepare different kinds of isotropic tissue engineering (TE) scaffolds. However, little attention has been paid to anisotropic porous scaffolds in spite of many examples of their excellent performances. In this work, a facile method termed "ammonia-induced method" (AIM) was proposed and applied to generate anisotropic pores in chitosan (CS)-based scaffolds. The pore structures of these scaffolds were studied in detail. In order to clarify the rationale behind this process, a speculative explanation was provided on basis of the experimental results and the theory of Uras (Uras & Devlin, 2000). Compression tests indicated that the mechanical strengths of these scaffolds were sufficient for TE applications. In vitro cell culture showed that MC3T3-E1 cells cultivated in the pores of these scaffolds had positive proliferation potential. We anticipated that this novel AIM could inspire research not only in TE but also in other fields. PMID:27516311

  4. Mapping cultivable land from satellite imagery with clustering algorithms

    Science.gov (United States)

    Arango, R. B.; Campos, A. M.; Combarro, E. F.; Canas, E. R.; Díaz, I.

    2016-07-01

    Open data satellite imagery provides valuable data for the planning and decision-making processes related with environmental domains. Specifically, agriculture uses remote sensing in a wide range of services, ranging from monitoring the health of the crops to forecasting the spread of crop diseases. In particular, this paper focuses on a methodology for the automatic delimitation of cultivable land by means of machine learning algorithms and satellite data. The method uses a partition clustering algorithm called Partitioning Around Medoids and considers the quality of the clusters obtained for each satellite band in order to evaluate which one better identifies cultivable land. The proposed method was tested with vineyards using as input the spectral and thermal bands of the Landsat 8 satellite. The experimental results show the great potential of this method for cultivable land monitoring from remote-sensed multispectral imagery.

  5. Mass Cultivation of Freshwater Microalgae

    Czech Academy of Sciences Publication Activity Database

    Masojídek, Jiří; Torzillo, G.

    2014-01-01

    Roč. 2, č. 2 (2014), s. 1-13. ISSN 0000-0000 R&D Projects: GA MŠk ED2.1.00/03.0110; GA MŠk EE2.3.30.0059 Institutional support: RVO:61388971 Keywords : microalgae * mass cultivation * bio-active compounds Subject RIV: EE - Microbiology, Virology

  6. Cultivating Spiritual Reflectivity in Teachers.

    Science.gov (United States)

    Mayes, Clifford

    2001-01-01

    Discusses the need to address spiritual beliefs in teacher education, arguing that: because spiritual impulses are so primary for many people, they must be addressed in teacher education; educators must recognize and cultivate students' spiritual motivations to teach and foster reflectivity; and student teachers must learn what they can legally…

  7. Mushroom Cultivation in South Korea

    Directory of Open Access Journals (Sweden)

    Mustafa Kemal Soylu

    2016-03-01

    Full Text Available Mushroom cultivation in South Korea is increasing fast last decades. Mushroom cultivation of South Korea is 173577 tones and South Korea gains 800 million dollars income annually. Different kind of mushroom species are cultivated and 31% enoki mushroom (Flammulina velutipes, 26% king oyster (Pleurotus eryngii, 26% oyster mushroom (Pleurotus ostreatus, 13% white buton mushroom (Agaricus bisporus and 4% rest of the total mushroom Lentinula edodes, Ganoderma lucidum, Phellinus vb. are produced in Korea. 764 ha mushroom growing area was used for mushroom cultivation. 31% of total growing area is oyster mushroom (Pleurotus ostreatus and 14% king oyster (Pleurotus eryngii, 6% winter mushroom (Flummulina velutipes, 16% white button mushroom (Agaricus bisporus, 3% Phellinus, 3% reishii (Ganoderma lucidum and 27% other mushrooms. Mushroom is consumed frequently in Korea and mushroom consumption per person is 4.2 kg. Growing on log culture (oak mushroom, reishi and Phellinus, growing on shelves (Agaricus bisporus and Pleurotus ostreatus and bottle culture (Flummulina velutipes, Pleurotus ostreatus, Pleurotus eryngii are commonly used growing systems.

  8. Cultivating archives: meanings and identities

    NARCIS (Netherlands)

    E. Ketelaar

    2012-01-01

    By cultivating archives through successive activations, people and communities define their identities. In these activations, the meanings of archives are constructed and reconstructed. Archives are not a static artifact imbued with the record creator’s voice alone, but a dynamic process involving a

  9. Systems and methods for interpolation-based dynamic programming

    KAUST Repository

    Rockwood, Alyn

    2013-01-03

    Embodiments of systems and methods for interpolation-based dynamic programming. In one embodiment, the method includes receiving an object function and a set of constraints associated with the objective function. The method may also include identifying a solution on the objective function corresponding to intersections of the constraints. Additionally, the method may include generating an interpolated surface that is in constant contact with the solution. The method may also include generating a vector field in response to the interpolated surface.

  10. Adaptability and stability of soybean genotypes in off-season cultivation.

    Science.gov (United States)

    Batista, R O; Hamawaki, R L; Sousa, L B; Nogueira, A P O; Hamawaki, O T

    2015-01-01

    The oil and protein contents of soybean grains are important quantitative traits for use in breeding. However, few breeding programs perform selection based on these traits in different environments. This study assessed the adaptability and stability of 14 elite early soybean breeding lines in off-season cultivation with respect to yield, and oil and protein contents. A range of statistical methods was applied and these analyses indicated that for off-season cultivation, the lines UFUS 5 and UFUS 10 could be recommended due to their superior performance in grain yield, oil content, and specific adaptability to unfavorable environments along with high stability in these characteristics. Also recommended were UFUS 06, which demonstrated superior performance in all three tested characteristics and showed adaptation to favorable environments, and UFUS 13, which showed high adaptability and stability and a superior performance for protein content. PMID:26345896

  11. Current situation for cultivating reflective thinking in English reading instruction--The influence of teaching models

    Institute of Scientific and Technical Information of China (English)

    Meng Qinglan

    2013-01-01

    Reflective thinking plays an essential role in cultivating students’creative ability. It’s essential to cultivate this ability in the process of reading instruction,while the present traditional English teaching models hinder its development. This paper tries to analyze the current situation in cultivating students’reflective thinking in English reading instruction from the traditional teaching models,in the hope of refreshing the teachers’consciousness on their teaching methods.

  12. Indigenous floating cultivation : a sustainable agricultural practice in the wetlands of Bangladesh.

    OpenAIRE

    Isalm, T.; Atkins, P.J.

    2007-01-01

    Floating-bed cultivation has proved a successful means to produce agricultural crops in various wetland areas of the world. In freshwater lakes and wetlands, vegetables, flowers, and seedlings are grown in Bangladesh using this floating cultivation technique, without any additional irrigation or chemical fertiliser. No detailed study of this indigenous cultivation technique has been published to date, although the laboratory method, hydroponics, is well documented in the professional literatu...

  13. Phenotypic variation in cultivated populations of Azadirachta indica in Yunnan, China

    OpenAIRE

    PENG Xing-Min; WU Jiang-Chong; ZHENG Yi-Xing; Zhang, Yan-Ping; LI Gen-Qian

    2012-01-01

    Aims Our objectives were to investigate phenotypic variation of Azadirachta indica in cultivated populations and to describe the relationship between phenotypic variation of the cultivated populations and different distribution areas.Methods We investigated 14 phenotypic traits for 90 individuals in nine cultivated populations of A. indica from Yunnan Province of China and used variance analysis, nested analysis, correlation analysis, principal component analysis, UPGMA cluster analysis and m...

  14. A Possible Industrial Solution to Ferment Lignocellulosic Hydrolyzate to Ethanol: Continuous Cultivation with Flocculating Yeast

    OpenAIRE

    Taherzadeh, Mohammad J.; Tomas Brandberg; Ronny Purwadi

    2007-01-01

    The cultivation of toxic lignocellulosic hydrolyzates has become a challenging research topic in recent decades. Although several cultivation methods have been proposed, numerous questions have arisen regarding their industrial applications. The current work deals with a solution to this problem which has a good potential application on an industrial scale. A toxic dilute-acid hydrolyzate was continuously cultivated using a high-cell-density flocculating yeast in a single and serial bioreacto...

  15. Decision Making Method Based on Paraconsistent Annotated Logic and Statistical Method: a Comparison

    Science.gov (United States)

    de Carvalho, Fábio Romeu; Brunstein, Israel; Abe, Jair Minoro

    2008-10-01

    Presently, there are new kinds of logic capable of handling uncertain and contradictory data without becoming trivial. Decision making theories based on these logics have shown to be powerful in many aspects regarding more traditional methods based on Statistics. In this paper we intend to outline a first study for a decision making theory based on Paraconsistent Annotated Evidential Logic Eτ (Paraconsistent Decision Method (PDM)) and classical Statistical Decision Method (SDM). Some discussion is presented below.

  16. Cultivating the thinking mode of evidence-based medicine for retinal postgraduate students%循证医学实践与眼底病专业研究生循证思维的培养

    Institute of Scientific and Technical Information of China (English)

    张新媛

    2012-01-01

    With the developments in the fields of medicine and biomedicine, physicians are not only required to have a high level of clinical skills, but more emphased to have understanding, analysis, synthesis, inferring and practicing abilities to adapt to the needs of modern medicine. The modern evidence-based practice emphasizes the "student-based, problem-based" educational model. Raising clinical questions using the PICOT (population, intervention, comparator, outcome, time) principle is extremely important for the medical graduates in their clinical practice training, especially for the retinal graduate students. Utilizing the evidence-based medicine resources as much as they can, practicing actively, cultivating the science of clinical thinking are all necessary. Cultivating the evidence-based thinking mode has special significance for those who want to be an excellent retinal specialist. (Ophthalmol CHN, 2012, 21: 357-359)%面对医学及生物医药领域发展的突飞猛进,循证医学不仅仅强调医生具有高水平的临床技能,更多地强调其具备认识、分析、综合、推断总结和实践的多种能力,以适应现代医学的需要.现代循证医学教育的模式强调“以学生为中心,以问题为中心”.眼底病专科学位研究生临床实践的培养应从平日的临床教育及工作中注意以人群、干预、对照、结果、时间(population,intervention,comparator,outcome,time,PICOT)原则凝练临床问题,注重学习利用循证医学资源,积极实践,在临床实践中培养科学的临床思维,以备在今后眼底病的临床及科研工作中发挥优势.培养良好的循证思维对于一名眼底病医师的成长具有深远意义.

  17. A visual saliency based method for vehicle logo detection

    Science.gov (United States)

    Zhang, Fan; Shen, Yiping; Chang, Hongxing

    2013-07-01

    This paper presents a novel method based on visual saliency and template matching for detecting vehicle logo from images captured by cross-road cameras. To detect the logo, such method first generates a saliency map based on the modified Itti's saliency model, and then obtains regions of interest (ROI) by thresholding the saliency map, at last performs an edge-based template matching to locate the logo. Experiments on more than 2400 images validate both high accuracy and efficiency of the proposed method, and demonstrates our method suitable for real-time application.

  18. 基于DEA模型的山东省耕地投入产出效率研究%RESEARCH ON INPUT-OUTPUT EFFICIENCY OF CULTIVATED LAND IN SHANDONG PROVINCE BASED ON DEA

    Institute of Scientific and Technical Information of China (English)

    宫攀; 韩振铃

    2015-01-01

    Cultivated land recourses was the most important natural resources, which was the basic guarantee of food security. Shandong province was one of China's most important bases of commodity grain production. In 2010, the arable land in Shandong was 7 , 510 , 761 hectares which accounting for 64. 95%of agricultural land and 47. 8%of the land area. The total output of Shandong in 2010 was 43. 53 million tons of grain, accounting for 7. 97%of total output in China. Firstly,this paper constructed the input and output index system with the input and output of cultivated land in Shandong province combined with availability of data and the actual situation. This index system divided into in-puts,outputs two levels. The output index selected economic efficiency and the social efficiency. The economic effi-ciency index included the output value of planting industry which reflected the scale of planting in study area,and value added of farming which expressed operation activity and service achievement. The social efficiency index re-flected by the grain output which mainly considerate that the social benefits of the arable land was mainly to main-tain social stability,and ensure food security. For investment-type indicators selected agricultural production inputs, labor inputs and land inputs. Secondly,this paper analyzed the change trend of the data of Shan Dong province's 17 municipalities during 2001~2010. Thirdly,the paper calculated the cultivated land use efficiency by the DEA mod-el using the data of Shan Dong province in 11 years. Besides,it had investigated the influences of the scale and pure technical factor on cultivated land use efficiency in different areas. The result showed that the output indexes including the total agricultural output value and the grain output all presented general increasing trend. According to the input indexes,from 2000 to 2010, 8 municipalities' crop sown area decreased while 9 of them increased;All the regions have improved their

  19. Novel orchid varieties cultivated

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ A research team led by Prof.DUAN Jun from the CAS South China Botanical Garden has been successful in breeding new orchid hybrids. One of them,Haraenopsis Nanzhi Pink (coming from Phalaenopsis Wedding Promenade and Haraella retrocalla) has been accepted as a new hybrid genus by the London-based Royal Horticulture Society (RHS). It is the first new hybrid genus successfully registered at RHS by orchid breeders from Chinese mainland.

  20. Method for detecting software anomalies based on recurrence plot analysis

    Directory of Open Access Journals (Sweden)

    Michał Mosdorf

    2012-03-01

    Full Text Available Presented paper evaluates method for detecting software anomalies based on recurrence plot analysis of trace log generated by software execution. Described method for detecting software anomalies is based on windowed recurrence quantification analysis for selected measures (e.g. Recurrence rate - RR or Determinism - DET. Initial results show that proposed method is useful in detecting silent software anomalies that do not result in typical crashes (e.g. exceptions.

  1. Economic Models of Shifting Cultivation: A Review

    OpenAIRE

    Takasaki, Yoshito

    2012-01-01

    This chapter reviews farm-level economic models of shifting cultivation and those of deforestation and soil conservation related to shifting cultivation. Although economists have made significant progress in modeling shifting cultivation over the last two decades, extant economic models neither clearly distinguish between primary and secondary forests nor address potential roles of on-farm soil conservation in shifting cultivation. Developing a unified farm model of primary forest clearing, f...

  2. Classification-Based Method of Linear Multicriteria Optimization

    OpenAIRE

    Vassilev, Vassil; Genova, Krassimira; Vassileva, Mariyana; Narula, Subhash

    2003-01-01

    The paper describes a classification-based learning-oriented interactive method for solving linear multicriteria optimization problems. The method allows the decision makers describe their preferences with greater flexibility, accuracy and reliability. The method is realized in an experimental software system supporting the solution of multicriteria optimization problems.

  3. Teaching based on the case method of building collective protection

    OpenAIRE

    Gonzalez Garcia, Nieves; Cobo Escamilla, Alfonso; Moreno Fernandez, Maria Esther

    2013-01-01

    One of the fundamental aspects in the adaptation of the teaching to the European higher education is changing based models of teacher education to models based on student learning. In this work we present an educational experience developed with the teaching method based on the case method, with a clearly multidisciplinary. The experience has been developed in the teaching of analysis and verification of safety rails. This is a multidisciplinary field that presents great difficulties during t...

  4. On the Cultivation of Intensive Reading Skills

    Institute of Scientific and Technical Information of China (English)

    杨雪琴

    2008-01-01

    This paper concerns the importance of the cultivation of reading skills,the characteristics of intensive reading and how to cultivate the intensive reading skills.Since the focus of this papre is how to cultivate the intensive reading skills,we make a systematic exposition from three points: word study,grammar patterns and text.

  5. Cultivating Students' English Autonomous Learning Ability in Web-based Environment%网络环境下大学生英语自主学习能力培养

    Institute of Scientific and Technical Information of China (English)

    张兴华; 杨洪娟

    2014-01-01

    该文分析网络环境下大学生英语自主学习存在的问题,并从网络环境下教师角色转变、课堂活动开展、校园网络资源平台建设、学生自主学习能力培训和指导、学生自主学习过程监控、评价、激励等机制指出如何培养网络环境下大学生英语自主学习能力。%This paper analyzes the problems of college students' autonomous learning in the web-based environment and points out how to cultivate students' English autonomous learning ability in the web-based environment from the changing role of teachers in the web-based environment, the launching of classroom activities, the construction of the campus network resourc-es platform, the training and guidance of the students' autonomous learning ability, autonomous learning process monitoring, evaluation, incentives and other mechanisms.

  6. Deciphering Community Structure of Methanotrophs Dwelling in Rice Rhizospheres of an Indian Rice Field Using Cultivation and Cultivation-Independent Approaches.

    Science.gov (United States)

    Pandit, Pranitha S; Rahalkar, Monali C; Dhakephalkar, Prashant K; Ranade, Dilip R; Pore, Soham; Arora, Preeti; Kapse, Neelam

    2016-04-01

    Methanotrophs play a crucial role in filtering out methane from habitats, such as flooded rice fields. India has the largest area under rice cultivation in the world; however, to the best of our knowledge, methanotrophs have not been isolated and characterized from Indian rice fields. A cultivation strategy composing of a modified medium, longer incubation time, and serial dilutions in microtiter plates was used to cultivate methanotrophs from a rice rhizosphere sample from a flooded rice field in Western India. We compared the cultured members with the uncultured community as revealed by three culture-independent methods. A novel type Ia methanotroph (Sn10-6), at the rank of a genus, and a putative novel species of a type II methanotroph (Sn-Cys) were cultivated from the terminal positive dilution (10(-6)). From lower dilution (10(-4)), a strain of Methylomonas spp. was cultivated. All the three culture-independent analyses, i.e., pmoA clone library, terminal restriction fragment length polymorphism (T-RFLP), and metagenomics approach, revealed the dominance of type I methanotrophs. Only metagenomic analysis showed significant presence of type II methanotrophs, albeit in lower proportion (37 %). All the three isolates showed relevance to the methanotrophic community as depicted by uncultured methods; however, the cultivated members might not be the most dominant ones. In conclusion, a combined cultivation and cultivation-independent strategy yielded us a broader picture of the methanotrophic community from rice rhizospheres of a flooded rice field in India. PMID:26547567

  7. Question Categorization Using SVM Based on Different Term Weighting Methods

    Directory of Open Access Journals (Sweden)

    Priyanka G Pillai

    2012-05-01

    Full Text Available This paper deals with the performance of Question Categorization based on four different term weighting methods. Term weighting methods such as tf*idf, qf*icf, iqf*qf*icf and vrf together with SVM classifier were used for categorization. From the experiments conducted using both linear and nonlinear SVM, term weighting method iqf*qf*icf showed better performance in question categorization than other methods.

  8. Question Categorization Using SVM Based on Different Term Weighting Methods

    OpenAIRE

    Priyanka G Pillai; Jayasree Narayanan

    2012-01-01

    This paper deals with the performance of Question Categorization based on four different term weighting methods. Term weighting methods such as tf*idf, qf*icf, iqf*qf*icf and vrf together with SVM classifier were used for categorization. From the experiments conducted using both linear and nonlinear SVM, term weighting method iqf*qf*icf showed better performance in question categorization than other methods.

  9. Conceptual bases of the brand valuation by cost method

    Directory of Open Access Journals (Sweden)

    G.Y. Studinska

    2015-03-01

    Full Text Available The necessity of valuing intangible assets in accordance with international trends is substantiated. The brand is seen as more important component of intangible assets, as an effective management tool company. The benefits and uses of brand evaluation results are investigated. System monocriterion cost brand evaluation methods is analyzed. In particular, methods that require evaluation by the time factor (current and forecast methods and methods for factor comparison base (relative and absolute. The cost method of brand valuation through market transactions in accordance J.Common’s classification is considered in detail. The explanation of the difference between method a summation of all costs and method of brand valuation through market transactions is provided. The advantages and disadvantages considered cost method of brand valuation are investigated. The cost method as the relative-predicted of the brand valuation, «The method of determining the proportion of the brand from the discounted total costs» is grounded

  10. A Spatialization-based Method for Checking and Updating Metadata

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    In this paper the application of spatialization technology on metadata quality check and updating was discussed. A new method based on spatialization was proposed for checking and updating metadata to overcome the deficiency of text based methods with the powerful functions of spatial query and analysis provided by GIS software. This method employs the technology of spatialization to transform metadata into a coordinate space and the functions of spatial analysis in GIS to check and update spatial metadata in a visual environment. The basic principle and technical flow of this method were explained in detail, and an example of implementation using ArcMap of GIS software was illustrated with a metadata set of digital raster maps. The result shows the new method with the support of interaction of graph and text is much more intuitive and convenient than the ordinary text based method, and can fully utilize the functions of GIS spatial query and analysis with more accuracy and efficiency.

  11. 基于生态足迹的甘肃省耕地资源可持续利用与情景预测%Sustainable use and scenario prediction of cultivated land in Gansu Province based on ecological footprint theory

    Institute of Scientific and Technical Information of China (English)

    刘秀丽; 张勃; 昝国江; 何旭强; 张调风

    2013-01-01

    基于生态足迹理论,测算了1997-2009年甘肃省在保持社会经济可持续发展下的最佳耕地资源容量,运用ARIMA模型对甘肃省2010-2015年的生态足迹和生态承载力进行预测,按照《甘肃省国民经济和社会发展第十二个五年规划纲要》中的社会经济指标对耕地资源可持续发展容量进行情景预测.结果表明:1997-2009年,甘肃省耕地资源可持续发展容量总体上呈现上升趋势,其中1997-2002年耕地资源可持续发展容量基本低于实际耕地面积,2003-2009年,耕地资源可持续发展容量均高于实际耕地面积,出现耕地利用负荷现象;ARIMA模型预测结果显示,2010-2015年,甘肃省人均耕地生态足迹呈现上升趋势,年平均增长率为4.75%,人均耕地生态承载力呈现减少趋势,但总体上变化不大,人均耕地生态赤字从2011年成为负值,并且逐渐增加;情景预测结果显示2010-2015年,甘肃省耕地资源可持续发展容量与实际耕地面积差距愈来愈大,预计到2015年,为实际耕地面积的2.42倍,耕地利用负荷现象严重,人地关系紧张.%Cultivated land resources, as parts of important natural resources, are the bases of existence and development of human society, which are also factors to the sustainable development of society. With the fast economic development and increasing population in China, the relationship between cultivated land resources and sustainable development of society is becoming more intensive. At the background of maintaining sustainable socio-economic development, using Ecological Footprint theory, the paper calculated the best capacity of cultivated land resources of Gansu province in from 1997 to 2009. And using the ARIMA model,it predicted the ecological footprint and ecological capacity of Gansu Province from 2010 to 2015. According to the socio-economic indicators in the 12th Five-Year Plan of Gansu Province, it gave a scenarios prediction of the sustainable

  12. Theory-Based Lexicographical Methods in a Functional Perspective

    DEFF Research Database (Denmark)

    Tarp, Sven

    2014-01-01

    This contribution provides an overview of some of the methods used in relation to the function theory. It starts with a definition of the concept of method and the relation existing between theory and method. It establishes an initial distinction between artisanal and theory-based methods and...... various methods used in the different sub-phases of the overall dictionary compilation process, from the making of the concept to the preparation for publication on the chosen media, with focus on the Internet. Finally, it briefly discusses some of the methods used to create and test the function theory...

  13. Data Mining and Knowledge Discovery via Logic-Based Methods

    CERN Document Server

    Triantaphyllou, Evangelos

    2010-01-01

    There are many approaches to data mining and knowledge discovery (DM&KD), including neural networks, closest neighbor methods, and various statistical methods. This monograph, however, focuses on the development and use of a novel approach, based on mathematical logic, that the author and his research associates have worked on over the last 20 years. The methods presented in the book deal with key DM&KD issues in an intuitive manner and in a natural sequence. Compared to other DM&KD methods, those based on mathematical logic offer a direct and often intuitive approach for extracting easily int

  14. Network Traffic Anomalies Identification Based on Classification Methods

    Directory of Open Access Journals (Sweden)

    Donatas Račys

    2015-07-01

    Full Text Available A problem of network traffic anomalies detection in the computer networks is analyzed. Overview of anomalies detection methods is given then advantages and disadvantages of the different methods are analyzed. Model for the traffic anomalies detection was developed based on IBM SPSS Modeler and is used to analyze SNMP data of the router. Investigation of the traffic anomalies was done using three classification methods and different sets of the learning data. Based on the results of investigation it was determined that C5.1 decision tree method has the largest accuracy and performance and can be successfully used for identification of the network traffic anomalies.

  15. Body Cultivation in Contemporary China

    OpenAIRE

    Palmer, DA

    2006-01-01

    Body cultivation practices known as qigong1 were the most widespread form of popular religiosity in post-Mao urban China between 1979 and 19992. It is estimated that at the height of “qigong fever” in the middle of this period, over 100 million people -- over 20% of the urban population -- practiced the gymnastic, breathing and meditative exercises of qigong in some form or another. Hundreds of charismatic qigong healers and masters rose to fame and built organisations which, i...

  16. Teaching Model by Associating Production Enterprises with College and Research Based on Students Ability and Quality Cultivation%基于学生能力和素质培养的产学研联动教学模式

    Institute of Scientific and Technical Information of China (English)

    赵玲; 黄大荣

    2012-01-01

    基于学生能力及素质培养的产学研联动教学模式,促进产学研三方受益,是目前高校教学和企业用人过程中遇到的难题。针对目前高校学生的实际情况,分析了目前教学过程中存在的问题以及产生问题的原因,从学生培养的基础理论课程学习、实践课程学习以及毕业论文设计3个环节,结合重庆交通大学教学的实践经验,提出了一些可供教师教学借鉴的可行建议。%The problem faced by higher learning institutions and enterprises in the process of employee recruitment is how to boost the benefit of teaching model to production enterprises, colleges and research based on students' ability and quality cultivation. This paper analyzes the problems in current teaching process and the cause for the problems based on the reality of colleges and universities, and provides feasible suggestions for teachers with teaching reference from students theoretical curriculum learning, practical course learning and graduate thesis design and on the basis of the practical experience in the teaching in Chongqing Jiaotong University.

  17. 基于利益相关者理论的企业社会责任的战略培育%Strategy Cultivating of Enterprise Social Responsibility Based on the Stakeholder Theory

    Institute of Scientific and Technical Information of China (English)

    肖功为; 曾海燕

    2012-01-01

    The stakeholder theory has rich enterprise social responsibility thought.It is a new important theoretical base for us to study the social responsibility of the enterprise.Based on the stakeholder theory,the enterprise social responsibility has five characteristics: mutual benefit,internalizing externality,voluntary and mandatory,associate gradient and social signal transitivity.Combining the enterprise social responsibility with the stakeholder theory,this paper lays emphasis on the charateristics of the enterprise social responsibility,the theoretical interpretation and the strategy cultivating.%利益相关者理论具有丰富的企业社会责任思想,是研究企业社会责任问题的一个新的重要理论基础。基于利益相关者理论的企业社会责任具有五个特征:互惠互利;"外部性"内部化;自愿性与强制性;关联梯度性;社会信号传递性。文章把利益相关者理论与企业社会责任研究结合起来研究,着重论述企业社会责任的特征、理论诠释及其战略培育。

  18. 基于创新教育的高职人才培养思路的探索%Exploration on Ways of Talents Cultivation Based on Innovative Education at Higher Vocational College

    Institute of Scientific and Technical Information of China (English)

    杨肖宁

    2015-01-01

    The importance of innovative talents is increasingly prominent in National Development Strategy with the fierce competition of comprehensive national power when entering into the 21st century. As the important base of talents training, colleges and universities play an important role in the process of cultivating innovative talents and constructing an innovative country. But the university talent-cultivating model in our country especially in higher vocational colleges still has a lot of problems unsuitable to the development of social economy. Due to this improper model the talents with weak creative ability lacking thoughts and motivations of diligent in thinking, venture in doubting and bravery in creating. Based on the view of problem solving, the writer of this paper believes that it is necessary to reform the present model and the paper provides some ways of exploring innovative talents at higher vocational colleges.%进入21世纪以来,随着国家间综合国力竞争的日趋激烈,创新型人才在国家发展战略中的重要性日渐突出.高校作为人才培养的重要基地,在培养创新型人才、建设创新型国家的进程中担负着重要使命.但是我国高校尤其是高职院校还存在许多不适应社会经济发展的地方,由此造成培养出来的人才创新能力不强,缺乏勤于思考、敢于怀疑、勇于创新的思想和动力.要解决这些问题,必须进行人才培养模式的改革.文章基于此出发点,对高职院校创新型人才培养的困境、原因、基本思路和措施等方面进行了探讨,为如何培养创新型人才提供了思路探索.

  19. MULTI-TRAIT EVALUATION OF VALUE FOR CULTIVATION AND USE OF EARLY MATURING EDIBLE POTATO CULTIVARS REGISTERED IN POLAND

    OpenAIRE

    Katarzyna Rymuza

    2014-01-01

    The work presents an analysis of diversity and comparison of value for cultivation and use of early maturing potato cultivars registered with the Polish National Register of Cultivars. The comparison was based on 17 yield and appearance traits and quality attributes of tubers as well as their resistance to diseases. The analysis employed the following multi-dimensional statistical methods: principal component analysis and cluster analysis. The principal component analysis revealed that over 7...

  20. Ellipse-based shape description and retrieval method

    Institute of Scientific and Technical Information of China (English)

    李向阳; 潘云鹤

    2002-01-01

    Using a group of ellipses to approach the shape contour, a new shape retrieval method is presented in this paper. In order to keep shape-based retrieval invariant to its position, orientation and size, the shape normalization method is presented. From our research, any closed shape contour can be uniquely decomposed into a group of ellipses, and the original shape contour can be re-constructed using the decomposed ellipses. The ellipse-based shape description and similar retrieval method is introduced in this paper. Based on ellipse's contribution to shape contour, the decomposed ellipses are parted into low-order ellipses and high-order ellipses. The low-order ellipses measure the macroscopic feature of a shape contour, and the high-order ellipses measure the microscopic feature. The two-phase shape matching method is given. Through the experiment test, our method has better shape retrieval effect.

  1. ENHANCED GRAPH BASED NORMALIZED CUT METHODS FOR IMAGE SEGMENTATION

    Directory of Open Access Journals (Sweden)

    S.D. Kapade

    2014-11-01

    Full Text Available Image segmentation is one of the important steps in digital image processing. Several algorithms are available for segmenting the images, posing many challenges such as precise criteria and efficient computations. Most of the graph based methods used for segmentation depend on local properties of graphs without considering global impressions of image, which ultimately limits segmentation quality. In this paper, we propose an enhanced graph based normalized cut method for extracting global impression and consistencies in the image. We propose a technique to add flexibility to original recursive normalized two way cut method which was further extended to other graph based methods. The results show that the proposed technique improves segmentation quality as well as requires lesser computational time than the regular normalized cut method.

  2. Metamorphic computer virus detection by Case- Based Reasoning (CBR) methods

    OpenAIRE

    Abdellatif Berkat

    2011-01-01

    Metamorphic virus employs code obfuscation techniques to mutate itself. It absconds from signaturebaseddetection system by modifying internal structure without compromising original functionality.In this paper, we propose a new method, for detecting metamorphic computer viruses, that is based on thetechnique of Case-Based Reasoning (CBR). In this method:-Can detect similar viruses with high probability.- The updating of the virus database is done automatically without connecting to the Intern...

  3. ENHANCED GRAPH BASED NORMALIZED CUT METHODS FOR IMAGE SEGMENTATION

    OpenAIRE

    S.D. Kapade; S.M. Khairnar; B.S. Chaudhari

    2014-01-01

    Image segmentation is one of the important steps in digital image processing. Several algorithms are available for segmenting the images, posing many challenges such as precise criteria and efficient computations. Most of the graph based methods used for segmentation depend on local properties of graphs without considering global impressions of image, which ultimately limits segmentation quality. In this paper, we propose an enhanced graph based normalized cut method for extracting global imp...

  4. Convergence of a residual based artificial viscosity finite element method

    KAUST Repository

    Nazarov, Murtazo

    2013-02-01

    We present a residual based artificial viscosity finite element method to solve conservation laws. The Galerkin approximation is stabilized by only residual based artificial viscosity, without any least-squares, SUPG, or streamline diffusion terms. We prove convergence of the method, applied to a scalar conservation law in two space dimensions, toward an unique entropy solution for implicit time stepping schemes. © 2012 Elsevier B.V. All rights reserved.

  5. Cultivating Microalgae in Domestic Wastewater for Biodiesel Production

    Directory of Open Access Journals (Sweden)

    Soha S.M. MOSTAFA

    2012-02-01

    Full Text Available The objective of this study was to evaluate the growth of nine species of microalgae (green and blue green microalgae on domestic waste water samples obtained from Zenein Waste Water Treatment Plant (ZWWTP, Giza governorate, Egypt. The species were cultivated in different kind of waste water; before treatment; after sterilization; with nutrients with sterilization and with nutrients without sterilization. The experiment was conducted in triplicate and cultures were incubated at 25�1�C under continuous shaking (150 rpm and illumination (2000 Lux for 15 days. pH, electric conductivity (EC, optical density (OD , dry weight (DW, were done at the time of incubation and at the end of experiment, in addition to determine the percentage of lipid and biodiesel. The data revealed that, domestic waste water with nutrient media (T3 was promising for cultivation of five algal species when compared with conventional media, Moreover, domestic waste water after sterilization (T2 was selected media for cultivation of Oscillatoria sp and Phormedium sp. However, T1 media (waste water without treatment was the promising media for cultivation of Nostoc humifusum. The biodiesel produced from algal species cultivated in waste water media ranged from 3.8 to 11.80% when compared with the conventional method (3.90 to 12.52%. The results of this study suggest that growing algae in nutrient rich media offers a new option of applying algal process in ZWWTP to mange the nutrient load for growth and valuable biodiesel feedstock production.

  6. Rapid quantification of viable Legionella in nuclear cooling tower waters using filter cultivation, fluorescent in situ hybridization, and solid phase cytometry

    OpenAIRE

    Baudart, Julia; Guillaume, C.; Mercier, A.; Lebaron, P.; Binet, M.

    2015-01-01

    To develop a rapid and sensitive method to quantify viable Legionella spp. in cooling tower water samples. A rapid, culture-based method capable of quantifying as few as 600 Legionella microcolonies per litre within 2 days in industrial waters was developed. The method combines a short cultivation step of microcolonies on GVPC agar plate, specific detection of Legionella cells by a fluorescent in situ hybridization (FISH) approach, and a sensitive enumeration using a solid-phase cytometer. Fo...

  7. Restrictive Factors of Vocational Education Development in Cultivation of Rural Practical Skilled Personnel and Countermeasures

    OpenAIRE

    Wang, Ning; Liu, Fang

    2014-01-01

    Kunming City is accelerating the process of agricultural modernization, industrialization, informationization and ecological development. In this process, it needs speeding up building new high level and advanced rural practical skilled personnel team suitable for development demand. By empirical analysis method, this paper discussed the factors restricting vocational education in cultivation of rural practical skilled personnel. Then, it came up with countermeasures in cultivation objective ...

  8. The afforestation problem: a heuristic method based on simulated annealing

    DEFF Research Database (Denmark)

    Vidal, Rene Victor Valqui

    1992-01-01

    This paper presents the afforestation problem, that is the location and design of new forest compartments to be planted in a given area. This optimization problem is solved by a two-step heuristic method based on simulated annealing. Tests and experiences with this method are also presented....

  9. A Channelization-Based DOA Estimation Method for Wideband Signals

    Directory of Open Access Journals (Sweden)

    Rui Guo

    2016-07-01

    Full Text Available In this paper, we propose a novel direction of arrival (DOA estimation method for wideband signals with sensor arrays. The proposed method splits the wideband array output into multiple frequency sub-channels and estimates the signal parameters using a digital channelization receiver. Based on the output sub-channels, a channelization-based incoherent signal subspace method (Channelization-ISM and a channelization-based test of orthogonality of projected subspaces method (Channelization-TOPS are proposed. Channelization-ISM applies narrowband signal subspace methods on each sub-channel independently. Then the arithmetic mean or geometric mean of the estimated DOAs from each sub-channel gives the final result. Channelization-TOPS measures the orthogonality between the signal and the noise subspaces of the output sub-channels to estimate DOAs. The proposed channelization-based method isolates signals in different bandwidths reasonably and improves the output SNR. It outperforms the conventional ISM and TOPS methods on estimation accuracy and dynamic range, especially in real environments. Besides, the parallel processing architecture makes it easy to implement on hardware. A wideband digital array radar (DAR using direct wideband radio frequency (RF digitization is presented. Experiments carried out in a microwave anechoic chamber with the wideband DAR are presented to demonstrate the performance. The results verify the effectiveness of the proposed method.

  10. A Channelization-Based DOA Estimation Method for Wideband Signals.

    Science.gov (United States)

    Guo, Rui; Zhang, Yue; Lin, Qianqiang; Chen, Zengping

    2016-01-01

    In this paper, we propose a novel direction of arrival (DOA) estimation method for wideband signals with sensor arrays. The proposed method splits the wideband array output into multiple frequency sub-channels and estimates the signal parameters using a digital channelization receiver. Based on the output sub-channels, a channelization-based incoherent signal subspace method (Channelization-ISM) and a channelization-based test of orthogonality of projected subspaces method (Channelization-TOPS) are proposed. Channelization-ISM applies narrowband signal subspace methods on each sub-channel independently. Then the arithmetic mean or geometric mean of the estimated DOAs from each sub-channel gives the final result. Channelization-TOPS measures the orthogonality between the signal and the noise subspaces of the output sub-channels to estimate DOAs. The proposed channelization-based method isolates signals in different bandwidths reasonably and improves the output SNR. It outperforms the conventional ISM and TOPS methods on estimation accuracy and dynamic range, especially in real environments. Besides, the parallel processing architecture makes it easy to implement on hardware. A wideband digital array radar (DAR) using direct wideband radio frequency (RF) digitization is presented. Experiments carried out in a microwave anechoic chamber with the wideband DAR are presented to demonstrate the performance. The results verify the effectiveness of the proposed method. PMID:27384566

  11. A copula-based method for synthetic microarray data generation

    OpenAIRE

    Lew, Sergio; Solé-Casals, Jordi; Caiafa, Cesar F.; Bau i Macià, Josep

    2012-01-01

    In this work, we propose a copula-based method to generate synthetic gene expression data that account for marginal and joint probability distributions features captured from real data. Our method allows us to implant significant genes in the synthetic dataset in a controlled manner, giving the possibility of testing new detection algorithms under more realistic environments.

  12. Tomographs based on non-conventional radiation sources and methods

    International Nuclear Information System (INIS)

    Computer techniques for tomographic reconstruction of objects X-rayed with a compact plasma focus (PF) are presented. The implemented reconstruction algorithms are based on stochastic searching of solutions of Radon equation, using Genetic Algorithms and Monte Carlo methods. Numerical experiments using actual projections were performed concluding the feasibility of the application of both methods in tomographic reconstruction problem. (author)

  13. On Accepting and Affording Subjects in Economic Compensation of Cultivated Land Protection Based on Questionnaire Surveys%耕地保护经济补偿的接受和给付主体分析——基于110份接受主体和445份给付主体的问卷调查

    Institute of Scientific and Technical Information of China (English)

    牛海鹏; 许传阳; 李明秋; 张安录

    2011-01-01

    Defining accepting and affording subjects of cultivated land protection economic compensation is the key of constructing the economic compensation system and mechanisms of cultivated land protection. The internal and interregional accepting subjects of economic compensation of cultivated land protection were defined and analyzed based on the theory of externalities donator. Meanwhile, the internal and interregional affording subjects of economic compensation of cultivated land protection were also defined and analyzed based on the externalities acceptor and its action patterns. Results suggest that (1) The externalities of cultivated land protection consist of internal and interregional externalities in terms of different consumption subjects. The externality problem of cultivated land protection can be conceptually divided into the internal externality problem and interregional externality problem. The internal externality problem is generally influenced by internal and interregional externalities; 2) The subject of cultivated land protection can be divided into the management subject, social supervision subject, and utilization subject by patterns of the behavior and mode of action of the subject in cultivated land protection;3) The utilization subject of cultivated land may be defined as the accepting subject of internal economic compensation in cultivated land protection in terms of the characteristics and connotation of externalities donator. The key areas for cultivated land protection may be termed an agent donator as it has not the characteristics of externalities donator. It consists of all accepting subjects in internal economic compensation in the key areas, also referred to as interregional union accepting subject of economic compensation of cultivated land protection; 4) The affording subject involves internal and interregional individuals who gain ecological and social benefits from cultivated land protection. It is indicated that the outside area

  14. Cultivating Students' Critical Thinking Ability through Simplified Modal United Nations Conference

    Science.gov (United States)

    Gao, Cun

    2016-01-01

    Cultivating EFL learners' critical thinking ability is an urgent task for English teachers. To integrate the training of language skills and cultivation of critical thinking ability into one language course, the author designed an activity called simplified Modal United Nations conference, which is based on the revised Bloom's Taxonomy that…

  15. Allergenic Potential of Tomatoes Cultivated in Organic and Conventional Systems.

    Science.gov (United States)

    Słowianek, Marta; Skorupa, Marta; Hallmann, Ewelina; Rembiałkowska, Ewa; Leszczyńska, Joanna

    2016-03-01

    Tomatoes (Lycopersicon esculentum Mill.) are a widely consumed vegetables and contain many health beneficial micronutrients. Unfortunately, they may also cause adverse allergic reactions in sensitized people. Many studies, conducted in recent years, indicate that organically produced vegetables have higher nutritional value, improved sensory quality and contain more health-enhancing bioactive compounds than vegetables grown under the conventional system. However, the relation between organic methods of cultivation and allergenic potential of tomatoes has received little scientific attention. This study analyzed samples of five tomato cultivars taken from organic and conventional systems over three consecutive years. The content of profilin, Bet v 1 and lipid transfer protein (LTP) analogues in tomato samples was determined using an indirect ELISA assay. Substantial quantities of these proteins were found in certain cultivars across all three years of cultivation. On the basis of these findings, organically grown tomatoes appear to offer little advantage over conventionally cultivated plants in terms of reduced allergenic potential. PMID:26590604

  16. A Semantic Retrieval Method Based on the Fuzzy Reasoning

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    This paper gives a semantic fuzzy retrieval method of multimedia object,discusses the principle of fuzzy semantic retrieval technique,presents a fuzzy reasoning mechanism based on the knowledge base,and designs the relevant reasoning algorithms.Researchful results have innovative significance.

  17. Role-based Integration Method of Enterprise Information System

    Institute of Scientific and Technical Information of China (English)

    YU Ming-hui; FEI Qi; CHEN Xue-guang

    2002-01-01

    This paper analyzes the current situation of enterprise information system and methods of system integration at first. Then a role-based analyzing method is proposed. It can help confirm the keystone of the construction of information system and the direction of system integration. At last, a case study on theintegration of material dispatching information system in a large-scale project is presented briefly. It shows that this new method is more effective than the others are.

  18. Strategies to Cultivate Middle School Students’ Interest in Learning English

    Institute of Scientific and Technical Information of China (English)

    杨培月

    2015-01-01

    “To inspire and cultivate students’ interest in learning English” is listed as one of the first lines as the mission of the foundational education stage in English course in the new curriculum standard. English learning interest is directly related to the students’ English learning achievement, how to improve middle school students’ English study interest has raised a lot of discussions and agreements.In this paper, the author, puts forward some strategies of cultivating middle school students English study interest, respectively is: need addition strategy, aspire interest strategy, double-base interest strategy and content addition strategy.

  19. A method for selecting training samples based on camera response

    Science.gov (United States)

    Zhang, Leihong; Li, Bei; Pan, Zilan; Liang, Dong; Kang, Yi; Zhang, Dawei; Ma, Xiuhua

    2016-09-01

    In the process of spectral reflectance reconstruction, sample selection plays an important role in the accuracy of the constructed model and in reconstruction effects. In this paper, a method for training sample selection based on camera response is proposed. It has been proved that the camera response value has a close correlation with the spectral reflectance. Consequently, in this paper we adopt the technique of drawing a sphere in camera response value space to select the training samples which have a higher correlation with the test samples. In addition, the Wiener estimation method is used to reconstruct the spectral reflectance. Finally, we find that the method of sample selection based on camera response value has the smallest color difference and root mean square error after reconstruction compared to the method using the full set of Munsell color charts, the Mohammadi training sample selection method, and the stratified sampling method. Moreover, the goodness of fit coefficient of this method is also the highest among the four sample selection methods. Taking all the factors mentioned above into consideration, the method of training sample selection based on camera response value enhances the reconstruction accuracy from both the colorimetric and spectral perspectives.

  20. Local Coding Based Matching Kernel Method for Image Classification

    OpenAIRE

    Song, Yan; McLoughlin, Ian Vince; Dai, Li-rong

    2014-01-01

    This paper mainly focuses on how to effectively and efficiently measure visual similarity for local feature based representation. Among existing methods, metrics based on Bag of Visual Word (BoV) techniques are efficient and conceptually simple, at the expense of effectiveness. By contrast, kernel based metrics are more effective, but at the cost of greater computational complexity and increased storage requirements. We show that a unified visual matching framework can be developed to encompa...

  1. Cultivation of College Students'Network-Based Autonomous Learning Ability in English%基于网络的大学生英语自主学习能力的培养

    Institute of Scientific and Technical Information of China (English)

    赵琳

    2011-01-01

    自主学习强调学生在学习过程中的主体地位和独立行为,以人本主义心理学和建构主义心理学为理论基础发展而来。自主学习能力已成为人类生存的基本能力,但很多大学生在英语学习过程中表现为此能力缺失。这既有自身原因,又有客观因素。网络以其信息海量、交互性强等优势为培养学生自主学习提供了有效途径。随着网络技术对英语教学改革的推进,校园网络等硬件设施的建设以及教师自主性、网络课程等软环境的构建都对学生自主学习能力培养起到推动作用。最后,多元化的评价体系对于网络环境下英语自主学习能力的培养也是不可缺少的。%Autonomous learning emphasizes students' dominant position and independent behavior in the process of learning.It is evolved from the theories of humanistic and constructivist psychology.The autonomous learning ability has become a basic ability of human existence,but a lot of college students are in lack of this ability in English learning.It is not only for reasons by themselves,but also some objective elements.With its massive information,strong interaction and some other advantages,network provides an effective way for the cultivation of students' autonomous learning.As network technology has promoted the reform of English teaching,both the construction of campus network and other hardware facilities and the construction of teacher autonomy,network courses,and other soft factors will improve students' autonomous learning ability.Finally,a multiple evaluation system is also essential in cultivating autonomous learning ability in English based on the network environment.

  2. 2D geometric measurement method based on industrial CT images

    International Nuclear Information System (INIS)

    To achieve the non-destructive measurement of the internal structure of the objects, a kind of automatic dimension measuring method using industrial computed tomography (ICT) images was presented based on a threshold of edge extraction. First, a pretreatment of CT images was carried out Then, the best threshold segmentation method was used to extract edge, based on this work the automatic geometry measurement of the CT images was achieved. The results show that geometric measurement of images reaches to a certain degree of accuracy and meet the basic needs of accuracy and repeatability. Simultaneously this method may reduce the influence of artifacts. (authors)

  3. An Efficient Method for Reliability-based Multidisciplinary Design Optimization

    Institute of Scientific and Technical Information of China (English)

    Fan Hui; Li Weiji

    2008-01-01

    Design for modem engineering system is becoming multidisciplinary and incorporates practical uncertainties; therefore, it is necessary to synthesize reliability analysis and the multidiscipLinary design optimization (MDO) techniques for the design of complex engineering system. An advanced first order second moment method-based concurrent subspace optimization approach is proposed based on the comparison and analysis of the existing multidisciplinary optimization techniques and the reliability analysis methods. It is seen through a canard configuration optimization for a three-surface transport that the proposed method is computationally efficient and practical with the least modification to the current deterministic optimization process.

  4. On Design Experiment Teaching in Engineering Quality Cultivation

    Science.gov (United States)

    Chen, Xiao

    2008-01-01

    Design experiment refers to that designed and conducted by students independently and is surely an important method to cultivate students' comprehensive quality. According to the development and requirements of experimental teaching, this article carries out a study and analysis on the purpose, significance, denotation, connotation and…

  5. The Approaches to Cultivating College Students' Learning Autonomy of English

    Institute of Scientific and Technical Information of China (English)

    王凤娟

    2010-01-01

    Learner autonomy is a mainstream view in recent education research. In our country' s traditional English teaching, students are lack of autonomous learning. Cultivating learner autonomy is essential and urgent. This paper provides some referential autonomy teaching methods for college English teachers to adapt to the requirement of learner autonomy and quality education.

  6. The Cultivation of English Reading Ability in Secondary School

    Institute of Scientific and Technical Information of China (English)

    李晨希

    2011-01-01

    With the development of the society, more and more people start to study English. Reading ability can help students to know the current events at present in the whole world. Here I will discus the methods of how to cultivate students' the reading ability in secondary school.

  7. The Elaboration of Cultivating Learners' English Communicative Competence in China

    Science.gov (United States)

    Zhang, Yuxiang; Wang, Jiling

    2012-01-01

    The communicative competence is the ultimate goal of the Communicative Language Teaching (CLT), which is thought to be the eclectic approach in the place of other approaches and methods and which begins to gain momentum in the recent years. Hence, the comprehensive understanding of the communicative competence is prerequisite to the cultivation of…

  8. Biophysical Properties of Cultivated Pastures in the Brazilian Savanna Biome: An Analysis in the Spatial-Temporal Domains Based on Ground and Satellite Data

    Directory of Open Access Journals (Sweden)

    Fernando M. Araújo

    2013-01-01

    Full Text Available Brazil has the largest commercial beef cattle herd in the world, with cattle ranching being particularly prominent in the 200-million ha, Brazilian neotropical moist savanna biome, known as Cerrado, one of the world’s hotspots for biodiversity conservation. As decreasing productivity is a major concern affecting the Cerrado pasturelands, evaluation of pasture conditions through the determination of biophysical parameters is instrumental for more effective management practices and herd occupation strategies. Within this context, the primary goal of this study was the regional assessment of pasture biophysical properties, through the scaling of wet- and dry-season ground truth data (total biomass, green biomass, and % green cover via the combined use of high (Landsat-TM and moderate (MODIS spatial resolution vegetation index images. Based on the high correlation found between NDVI (normalized difference vegetation index and % green cover (r = 0.95, monthly MODIS-based % green cover images were derived for the 2009–2010 hydrological cycle, which were able to capture major regional patterns and differences in pasture biophysical responses, including the increasing greenness values towards the southern portions of the biome, due to both local conditions (e.g., more fertile soils and management practices. These results corroborate the development of biophysically-based landscape degradation indices, in support of improved land use governance and natural area conservation in the Cerrado.

  9. An Entropy-Based Network Anomaly Detection Method

    Directory of Open Access Journals (Sweden)

    Przemysław Bereziński

    2015-04-01

    Full Text Available Data mining is an interdisciplinary subfield of computer science involving methods at the intersection of artificial intelligence, machine learning and statistics. One of the data mining tasks is anomaly detection which is the analysis of large quantities of data to identify items, events or observations which do not conform to an expected pattern. Anomaly detection is applicable in a variety of domains, e.g., fraud detection, fault detection, system health monitoring but this article focuses on application of anomaly detection in the field of network intrusion detection.The main goal of the article is to prove that an entropy-based approach is suitable to detect modern botnet-like malware based on anomalous patterns in network. This aim is achieved by realization of the following points: (i preparation of a concept of original entropy-based network anomaly detection method, (ii implementation of the method, (iii preparation of original dataset, (iv evaluation of the method.

  10. Optimizing distance-based methods for large data sets

    Science.gov (United States)

    Scholl, Tobias; Brenner, Thomas

    2015-10-01

    Distance-based methods for measuring spatial concentration of industries have received an increasing popularity in the spatial econometrics community. However, a limiting factor for using these methods is their computational complexity since both their memory requirements and running times are in {{O}}(n^2). In this paper, we present an algorithm with constant memory requirements and shorter running time, enabling distance-based methods to deal with large data sets. We discuss three recent distance-based methods in spatial econometrics: the D&O-Index by Duranton and Overman (Rev Econ Stud 72(4):1077-1106, 2005), the M-function by Marcon and Puech (J Econ Geogr 10(5):745-762, 2010) and the Cluster-Index by Scholl and Brenner (Reg Stud (ahead-of-print):1-15, 2014). Finally, we present an alternative calculation for the latter index that allows the use of data sets with millions of firms.

  11. Kernel based eigenvalue-decomposition methods for analysing ham

    DEFF Research Database (Denmark)

    Christiansen, Asger Nyman; Nielsen, Allan Aasbjerg; Møller, Flemming;

    2010-01-01

    conditions and finding useful additives to hinder the color to change rapidly. To be able to prove which methods of storing and additives work, Danisco wants to monitor the development of the color of meat in a slice of ham as a function of time, environment and ingredients. We have chosen to use multi......Every consumer wants fresh ham and the way we decide whether the meat is fresh or not is by looking at the color. The producers of ham wants a long shelf life, meaning they want the ham to look fresh for a long time. The Danish company Danisco is therefore trying to develop optimal storing...... methods, such as PCA, MAF or MNF. We therefore investigated the applicability of kernel based versions of these transformation. This meant implementing the kernel based methods and developing new theory, since kernel based MAF and MNF is not described in the literature yet. The traditional methods only...

  12. Patch nearfield acoustic holography based on the equivalent source method

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    On the basis of nearfield acoustic holography (NAH) based on the equivalent source method (ESM), patch NAH based on the ESM is proposed. The method overcomes the shortcoming in the conventional NAH that the hologram surface should be larger than the source surface. It need not to discretize the whole source and its measurement need not to cover the whole source. The measurement may be performed over the region of interest, and the reconstruction can be done in the region directly. The method is flexible in applications, stable in computation, and very easy to implement. It has good potential applications in engineering. The numerical simulations show the invalidity of the conventional NAH based on the ESM and prove the validities of the proposed method for reconstructing a partial source and the regularization for reducing the error effect of the pressure measured on the hologram surface.

  13. Patch nearfield acoustic holography based on the equivalent source method

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    On the basis of nearfield acoustic holography (NAH) based on the equivalent source method (ESM), patch NAH based on the ESM is proposed. The method overcomes the shortcoming in the conventional NAH that the hologram surface should be larger than the source surface. It need not to discretize the whole source and its measurement need not to cover the whole source. The measurement may be performed over the region of interest, and the reconstruction can be done in the region directly. The method is flexible in applications, stable in computation, and very easy to implement. It has good potential applications in engineering. The nu- merical simulations show the invalidity of the conventional NAH based on the ESM and prove the validities of the proposed method for reconstructing a partial source and the regularization for reducing the error effect of the pressure measured on the hologram surface.

  14. On the Curriculum System of International Business Profession Based on the Cultivation of Professional Man%基于国际商务“职业人”培养的专业课程体系探讨

    Institute of Scientific and Technical Information of China (English)

    刘建明; 赵继梅

    2013-01-01

      The construction of curriculum is the core of teaching quality improvement. We should set the curriculum according to essential and enough principles and requirements of technical field and job post, determine the structure of professional knowledge centering on the cultivation of professional ability and quality. Based on the training objective of Professional Man of International Business, the original curriculum system of International Business profession should be reformed structurally, the curriculum system of professional characteristics should be explored and constructed to realize a real teaching reform.%  课程建设是提高教学质量的核心,要按照“必需、够用”的原则,根据技术领域和职业岗位的要求设置课程,围绕职业能力和素质培养确定所需的专业知识结构。基于国际商务“职业人”的培养目标,对原有的国际商务专业课程体系进行结构性改革,探索构建具有职业特色的课程体系,实现真正意义上的教学改革。

  15. Correction of Misclassifications Using a Proximity-Based Estimation Method

    Science.gov (United States)

    Niemistö, Antti; Shmulevich, Ilya; Lukin, Vladimir V.; Dolia, Alexander N.; Yli-Harja, Olli

    2004-12-01

    An estimation method for correcting misclassifications in signal and image processing is presented. The method is based on the use of context-based (temporal or spatial) information in a sliding-window fashion. The classes can be purely nominal, that is, an ordering of the classes is not required. The method employs nonlinear operations based on class proximities defined by a proximity matrix. Two case studies are presented. In the first, the proposed method is applied to one-dimensional signals for processing data that are obtained by a musical key-finding algorithm. In the second, the estimation method is applied to two-dimensional signals for correction of misclassifications in images. In the first case study, the proximity matrix employed by the estimation method follows directly from music perception studies, whereas in the second case study, the optimal proximity matrix is obtained with genetic algorithms as the learning rule in a training-based optimization framework. Simulation results are presented in both case studies and the degree of improvement in classification accuracy that is obtained by the proposed method is assessed statistically using Kappa analysis.

  16. Correction of Misclassifications Using a Proximity-Based Estimation Method

    Directory of Open Access Journals (Sweden)

    Shmulevich Ilya

    2004-01-01

    Full Text Available An estimation method for correcting misclassifications in signal and image processing is presented. The method is based on the use of context-based (temporal or spatial information in a sliding-window fashion. The classes can be purely nominal, that is, an ordering of the classes is not required. The method employs nonlinear operations based on class proximities defined by a proximity matrix. Two case studies are presented. In the first, the proposed method is applied to one-dimensional signals for processing data that are obtained by a musical key-finding algorithm. In the second, the estimation method is applied to two-dimensional signals for correction of misclassifications in images. In the first case study, the proximity matrix employed by the estimation method follows directly from music perception studies, whereas in the second case study, the optimal proximity matrix is obtained with genetic algorithms as the learning rule in a training-based optimization framework. Simulation results are presented in both case studies and the degree of improvement in classification accuracy that is obtained by the proposed method is assessed statistically using Kappa analysis.

  17. The importance of cultivating Metaphoric Competence in Teaching Reading

    Institute of Scientific and Technical Information of China (English)

    孙小瑶

    2015-01-01

    Metaphor is outstanding in Foreign Language Learning and Teaching.Based on discussing the reading model in reading teaching and the relationship between the metaphoric competence and reading comprehension,this paper advocated that teachers should cultivate students’ metaphoric competence to improve students’ reading proficiency.

  18. Cloning of Babesia bovis by in vitro cultivation.

    OpenAIRE

    Rodriguez, S D; Buening, G M; Green, T. J.; Carson, C A

    1983-01-01

    A procedure for cloning Babesia bovis was developed. The procedure was used to establish and cultivate homogeneous populations of parasites and to isolate B. bovis from carrier animals. Three different clone lines of B. bovis based on in vitro growth rates were established.

  19. Integrated navigation method based on inertial navigation system and Lidar

    Science.gov (United States)

    Zhang, Xiaoyue; Shi, Haitao; Pan, Jianye; Zhang, Chunxi

    2016-04-01

    An integrated navigation method based on the inertial navigational system (INS) and Lidar was proposed for land navigation. Compared with the traditional integrated navigational method and dead reckoning (DR) method, the influence of the inertial measurement unit (IMU) scale factor and misalignment was considered in the new method. First, the influence of the IMU scale factor and misalignment on navigation accuracy was analyzed. Based on the analysis, the integrated system error model of INS and Lidar was established, in which the IMU scale factor and misalignment error states were included. Then the observability of IMU error states was analyzed. According to the results of the observability analysis, the integrated system was optimized. Finally, numerical simulation and a vehicle test were carried out to validate the availability and utility of the proposed INS/Lidar integrated navigational method. Compared with the test result of a traditional integrated navigation method and DR method, the proposed integrated navigational method could result in a higher navigation precision. Consequently, the IMU scale factor and misalignment error were effectively compensated by the proposed method and the new integrated navigational method is valid.

  20. Métodos de superação da dormência de sementes de plantas daninhas de pastagens cultivadas da Amazônia Dormancy overcoming methods of weed seeds from Amazonian cultivated pasture

    Directory of Open Access Journals (Sweden)

    Antonio P. da S. Souza Filho

    1998-06-01

    Full Text Available O objetivo do presente trabalho foi o de avaliar diferentes métodos de superação da dormência de sementes de plantas daninhas de áreas de pastagens cultivadas da região amazônica brasileira. Foram estudados os métodos escarificação térmica em água a temperatura de 80oC por 4, 8 e 12 minutos, escarificação química em ácido sulfúrico por 5, 10, 15 e 20 minutos e nitrato de potássio nas concentrações de 0,1; 0,2 e 0,3%. As sementes, cuja dormência não foi superada por esses métodos, foram colocadas para germinar na presença de cinetina (20, 40, 60 e 80 ppm e de giberelina (150, 300, 450 e 600 ppm. A germinação foi monitorada em períodos de 15 dias, com contagem diária e eliminação das sementes germinadas. A escarificação térmica em água não se mostrou satisfatória, tendo havido, para a maioria das espécies, redução da germinação em relação ao tratamento testemunha. O ácido sulfúrico foi eficiente para superar a dormência das sementes de todas as espécies, havendo, no entanto, variações com relação ao tempo de imersão. O nitrato de potássio afetou positivamente a germinação das sementes de fedegoso, de rinchão e, mais expressivamente, de Hyptis mutabilis. As sementes de jurubebão responderam, positivamente, apenas aos diferentes níveis de giberelina, atingindo valor superior de germinação na concentração de 600 ppm.The aim of this work was to evaluate different dormancy overcoming methods of weed seeds of cultivated pastures in the Amazon region. The following methods were studied: Thermal scarification in hot water (800C for 4, 8 and 12 minutes; chemical scarification in sulfuric acid for 5, 10, 15 and 20 minutes and potassium nitrate in the concentrations of 0.1, 0.2 and 0.3%. The seeds that did not overcome dormancy in these methods were put to germinate in the presence of kinetin (20, 40, 60 and 80 ppm and gibberelic acid ( 150, 300, 450 and 600 ppm. Germination was monitorated during

  1. NETWORK INTRUSION DETECTION METHOD BASED ON RS-MSVM

    Institute of Scientific and Technical Information of China (English)

    Xiao Yun; Han Chongzhao; Zheng Qinghua; Zhang Junjie

    2006-01-01

    A new method called RS-MSVM (Rough Set and Multi-class Support Vector Machine) is proposed for network intrusion detection. This method is based on rough set followed by MSVM for attribute reduction and classification respectively. The number of attributes of the network data used in this paper is reduced from 41 to 30 using rough set theory. The kernel function of HVDM-RBF (Heterogeneous Value Difference Metric Radial Basis Function), based on the heterogeneous value difference metric of heterogeneous datasets, is constructed for the heterogeneous network data. HVDM-RBF and one-against-one method are applied to build MSVM. DARPA (Defense Advanced Research Projects Agency) intrusion detection evaluating data were used in the experiment. The testing results show that our method outperforms other methods mentioned in this paper on six aspects: detection accuracy, number of support vectors, false positive rate, false negative rate, training time and testing time.

  2. NONLINEAR DATA RECONCILIATION METHOD BASED ON KERNEL PRINCIPAL COMPONENT ANALYSIS

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    In the industrial process situation, principal component analysis (PCA) is a general method in data reconciliation.However, PCA sometime is unfeasible to nonlinear feature analysis and limited in application to nonlinear industrial process.Kernel PCA (KPCA) is extension of PCA and can be used for nonlinear feature analysis.A nonlinear data reconciliation method based on KPCA is proposed.The basic idea of this method is that firstly original data are mapped to high dimensional feature space by nonlinear function, and PCA is implemented in the feature space.Then nonlinear feature analysis is implemented and data are reconstructed by using the kernel.The data reconciliation method based on KPCA is applied to ternary distillation column.Simulation results show that this method can filter the noise in measurements of nonlinear process and reconciliated data can represent the true information of nonlinear process.

  3. Consistency-based ellipse detection method for complicated images

    Science.gov (United States)

    Zhang, Lijun; Huang, Xuexiang; Feng, Weichun; Liang, Shuli; Hu, Tianjian

    2016-05-01

    Accurate ellipse detection in complicated images is a challenging problem due to corruptions from image clutter, noise, or occlusion of other objects. To cope with this problem, an edge-following-based ellipse detection method is proposed which promotes the performances of the subprocesses based on consistency. The ellipse detector models edge connectivity by line segments and exploits inconsistent endpoints of the line segments to split the edge contours into smooth arcs. The smooth arcs are further refined with a novel arc refinement method which iteratively improves the consistency degree of the smooth arc. A two-phase arc integration method is developed to group disconnected elliptical arcs belonging to the same ellipse, and two constraints based on consistency are defined to increase the effectiveness and speed of the merging process. Finally, an efficient ellipse validation method is proposed to evaluate the saliency of the elliptic hypotheses. Detailed evaluation on synthetic images shows that our method outperforms other state-of-the-art ellipse detection methods in terms of effectiveness and speed. Additionally, we test our detector on three challenging real-world datasets. The F-measure score and execution time of results demonstrate that our method is effective and fast in complicated images. Therefore, the proposed method is suitable for practical applications.

  4. High-power LEDs for plant cultivation

    Science.gov (United States)

    Tamulaitis, Gintautas; Duchovskis, Pavelas; Bliznikas, Zenius; Breive, Kestutis; Ulinskaite, Raimonda; Brazaityte, Ausra; Novickovas, Algirdas; Zukauskas, Arturas; Shur, Michael S.

    2004-10-01

    We report on high-power solid-state lighting facility for cultivation of greenhouse vegetables and on the results of the study of control of photosynthetic activity and growth morphology of radish and lettuce imposed by variation of the spectral composition of illumination. Experimental lighting modules (useful area of 0.22 m2) were designed based on 4 types of high-power light-emitting diodes (LEDs) with emission peaked in red at the wavelengths of 660 nm and 640 nm (predominantly absorbed by chlorophyll a and b for photosynthesis, respectively), in blue at 455 nm (phototropic function), and in far-red at 735 nm (important for photomorphology). Morphological characteristics, chlorophyll and phytohormone concentrations in radish and lettuce grown in phytotron chambers under lighting with different spectral composition of the LED-based illuminator and under illumination by high pressure sodium lamps with an equivalent photosynthetic photon flux density were compared. A well-balanced solid-state lighting was found to enhance production of green mass and to ensure healthy morphogenesis of plants compared to those grown using conventional lighting. We observed that the plant morphology and concentrations of morphologically active phytohormones is strongly affected by the spectral composition of light in the red region. Commercial application of the LED-based illumination for large-scale plant cultivation is discussed. This technology is favorable from the point of view of energy consumption, controllable growth, and food safety but is hindered by high cost of the LEDs. Large scale manufacturing of high-power red AlInGaP-based LEDs emitting at 650 nm and a further decrease of the photon price for the LEDs emitting in the vicinity of the absorption peak of chlorophylls have to be achieved to promote horticulture applications.

  5. Optimal control of greenhouse cultivation

    NARCIS (Netherlands)

    Straten, van G.; Willigenburg, van L.G.; Henten, van E.J.; Ooteghem, van R.J.C.

    2011-01-01

    Features Discusses economic optimization of greenhouse control through mathematical modeling Examines 30 years of scientific research to present a unified framework for efficient decision-making Presents modern methods of control and optimization including classical rule-based and multivariable feed

  6. Color Restoration Method Based on Spectral Information Using Normalized Cut

    Institute of Scientific and Technical Information of China (English)

    Tetsuro Morimoto; Tohru Mihashi; Katsushi Ikeuchi

    2008-01-01

    This paper proposes a novel method for color restoration that can effectively apply accurate color based on spectral information to a segmented image using the normalized cut technique. Using the proposed method, we can obtain a digital still camera image and spectral information in different environments. Also, it is not necessary to estimate reflectance spectra using a spectral database such as other methods. The synthesized images are accurate and high resolution. The proposed method effectively works in making digital archive contents. Some experimental results are demonstrated in this paper.

  7. Analysis of fault detection method based on predictive filter approach

    Institute of Scientific and Technical Information of China (English)

    LI Ji; ZHANG Hongyue

    2005-01-01

    A new detection method for component faults based on predictive filters together with the fault detectability, false alarm rate, missed alarm rate and upper bound of detection time are proposed. The efficiency of the method is illustrated by a simulation example of a second-order system. It is shown that the fault detection method using predictive filters has a small delay, a low false alarm rate and a low missed alarm rate. Furthermore the filter can give accurate estimates of states even after a fault occurs. The real-time estimation provided by this method can also be used for fault tolerant control.

  8. Adulteration and cultivation region identification of American ginseng using HPLC coupled with multivariate analysis.

    Science.gov (United States)

    Yu, Chunhao; Wang, Chong-Zhi; Zhou, Chun-Jie; Wang, Bin; Han, Lide; Zhang, Chun-Feng; Wu, Xiao-Hui; Yuan, Chun-Su

    2014-10-01

    American ginseng (Panax quinquefolius) is originally grown in North America. Due to price difference and supply shortage, American ginseng recently has been cultivated in northern China. Further, in the market, some Asian ginsengs are labeled as American ginseng. In this study, forty-three American ginseng samples cultivated in the USA, Canada or China were collected and 14 ginseng saponins were determined using HPLC. HPLC coupled with hierarchical cluster analysis and principal component analysis was developed to identify the species. Subsequently, an HPLC-linear discriminant analysis was established to discriminate cultivation regions of American ginseng. This method was successfully applied to identify the sources of 6 commercial American ginseng samples. Two of them were identified as Asian ginseng, while 4 others were identified as American ginseng, which were cultivated in the USA (3) and China (1). Our newly developed method can be used to identify American ginseng with different cultivation regions. PMID:25044150

  9. High-throughput cultivation and screening platform for unicellular phototrophs

    OpenAIRE

    Tillich, Ulrich M; Wolter, Nick; Schulze, Katja; Kramer, Dan; Brödel, Oliver; Frohme, Marcus

    2014-01-01

    Background High-throughput cultivation and screening methods allow a parallel, miniaturized and cost efficient processing of many samples. These methods however, have not been generally established for phototrophic organisms such as microalgae or cyanobacteria. Results In this work we describe and test high-throughput methods with the model organism Synechocystis sp. PCC6803. The required technical automation for these processes was achieved with a Tecan Freedom Evo 200 pipetting robot. The c...

  10. a Minimum Spanning Tree Based Method for Uav Image Segmentation

    Science.gov (United States)

    Wang, Ping; Wei, Zheng; Cui, Weihong; Lin, Zhiyong

    2016-06-01

    This paper proposes a Minimum Span Tree (MST) based image segmentation method for UAV images in coastal area. An edge weight based optimal criterion (merging predicate) is defined, which based on statistical learning theory (SLT). And we used a scale control parameter to control the segmentation scale. Experiments based on the high resolution UAV images in coastal area show that the proposed merging predicate can keep the integrity of the objects and prevent results from over segmentation. The segmentation results proves its efficiency in segmenting the rich texture images with good boundary of objects.

  11. The Pickling Cucumbers Cultivated from Seedlings and Direct Sowing

    Directory of Open Access Journals (Sweden)

    Ivanka Žutić

    1998-12-01

    Full Text Available The start of infection with downy mildew (Pseudoperonospora cubensis Berk et Curt. coincides with the picking period start of pickling cucumbers (Cucumis sativus L. cultivated through direct sowing, while the application of fungicide is being limited through frequent harvests. The larger portion of cucumber crop grown from the seedlings could be harvested prior to the onset of intense downy mildew infection without fungicide application in the picking period. Cultivar Levina growth was investigated in a two years experiment from two and three weeks old seedlings, with a root's substratum volume of 100 and 200 cm 3 , in comparison with the direct sowing as a standard cultivation. In the cultivation method from the seedlings the plants were ready for harvest for two or three weeks earlier than obtainable from the direct sowing. As a consequence the harvest period by the seedlings method lasted for two weeks longer, whereby a higher market product yield was materialized. It was established that the seedling age, and root's substratum volume have no substantial influence on an earlier picking period or the height of market cucumber product yields either. In the picking period by the cucumber cultivation from the seedlings method and with a three times/week harvest, it becomes possible without fungicide application to get 75 % of the yield until the onset of the intense downy mildew infection.

  12. A flower image retrieval method based on ROI feature

    Institute of Scientific and Technical Information of China (English)

    洪安祥; 陈刚; 李均利; 池哲儒; 张亶

    2004-01-01

    Flower image retrieval is a very important step for computer-aided plant species recognition. In this paper, we propose an efficient segmentation method based on color clustering and domain knowledge to extract flower regions from flower images. For flower retrieval, we use the color histogram of a flower region to characterize the color features of flower and two shape-based features sets, Centroid-Contour Distance (CCD) and Angle Code Histogram (ACH), to characterize the shape features of a flower contour. Experimental results showed that our flower region extraction method based on color clustering and domain knowledge can produce accurate flower regions. Flower retrieval results on a database of 885 flower images collected from 14 plant species showed that our Region-of-Interest (ROI) based retrieval approach using both color and shape features can perform better than a method based on the global color histogram proposed by Swain and Ballard (1991) and a method based on domain knowledge-driven segmentation and color names proposed by Das et al.(1999).

  13. A flower image retrieval method based on ROI feature

    Institute of Scientific and Technical Information of China (English)

    洪安祥; 陈刚; 李均利; 池哲儒; 张亶

    2004-01-01

    Flower image retrieval is a very important step for computer-aided plant species recognition.In this paper,we propose an efficient segmentation method based on color clustering and domain knowledge to extract flower regions from flower images.For flower retrieval,we use the color histogram of a flower region to characterize the color features of flower and two shape-based features sets,Centroid-Contour Distance(CCD)and Angle Code Histogram(ACH),to characterize the shape features of a flower contour.Experimental results showed that our flower region extraction method based on color clustering and domain knowledge can produce accurate flower regions.Flower retrieval results on a database of 885 flower images collected from 14 plant species showed that our Region-of-Interest(ROD based retrieval approach using both color and shape features can perform better than a method based on the global color histogram proposed by Swain and Ballard(1991)and a method based on domain knowledge-driven segmentation and color names proposed by Das et al.(1999).

  14. Cultivation of Children's Self-dependence Ability%浅谈学生自理能力的培养

    Institute of Scientific and Technical Information of China (English)

    王晓静

    2011-01-01

    Self-care ability is the embodiment of the self service ability and plays an important role in children's growth.Based on the analysis of the infants' ability of self-dependence, the paper puts forward some cultivating methods of ability of self-dependence.The practice proves that this method can effectively improve their ability of self-dependence, establish confidence, and cultivate perfect personality.%自理能力是自我服务能力的体现,对幼儿的成长有着重要作用.本文通过对幼儿自理能力的分析,提出了一些培养幼儿自理能力的方法.经实践证明,这些方法能够有效提高幼儿自理能力,树立自信心,培养健全人格.

  15. Waste streams for algae cultivation

    OpenAIRE

    Kautto, Antti

    2011-01-01

    ALDIGA, short for “Algae from Waste for Combined Biodiesel and Biogas Pro-duction”, aims to develop a concept for a closed circulation of resources in pro-ducing biodiesel and biogas from waste. The project is realized in co-operation between VTT, University of Helsinki, Lahti and Häme Universities of Applied Sciences, SYKE and funded by Tekes. The project’s first work phase ergo this bachelor’s thesis covered the mapping of available and suitable streams to be used in the cultivation of ...

  16. Talent Cultivation Project Onthe Move

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    In the golden autumn of 2002, a ceremony was held in Lhasa for the Tibet Development Fund to donate to the Talent Cultivation Project. Nineteen Tibetan students currently studying in the hinterland and Tibetan universities and high schools, whose families live below the poverty line, formed the first group of recipients; 24 others belong to the second group to be aided. Radi (Chairman of the People’s Congress of the Tibet Autonomous Region), Lobsang Toinzhub (Vice-Chairman of the TAR government and advisor to the Tibet Development Fund), and Lhamin Soinam Lhunzhub (Vice-Chairman of the CPPCC Tibet Committee and council member of the Tibet Development Fund)

  17. Métodos para a climatização de bananas 'Prata-Anã' produzidas na Amazônia Setentrional Brasileira Methods of climatization of 'Prata-Anã' bananas cultivated in the Northern Brazilian Amazon

    Directory of Open Access Journals (Sweden)

    Marcos André de Souza Prill

    2012-12-01

    Full Text Available O objetivo, neste trabalho, foi avaliar o uso da climatização para a uniformização de bananas 'Prata-Anã' produzidas em Boa Vista-RR. Após colhidos, os frutos foram selecionados no formato de buquês, sanitizados, climatizados por abafamento com lona plástica ou por imersão em solução de Ethrel®, embalados com filme de polietileno de baixa densidade e armazenados por quatro períodos de tempo (0; 10;20 e 30 dias a 12 ± 1 ºC e 93 ± 2% de UR. Após cada período de armazenamento refrigerado (AR os frutos foram submetidos ao armazenamento em condições ambiente (22 ± 1 ºC e 75 ± 3% UR, retirados das embalagens plásticas e sendo analisados após 1; 2; 3 e 4 dias. As seguintes análises foram realizadas: perda de massa fresca, coloração da casca, produção de etileno e CO2, atividade das enzimas pectinametilesterase e poligalacturonase, acidez titulável (AT, pectina total e solúvel, amido e sólidos solúveis (SS. Não houve diferenças significativas entre os métodos de climatização, porém verificou-se que, quanto maior o período de AR e de condicionamento, menor foi o período de conservação das bananas 'Prata-Anã'. Ficou evidenciado, também, que a climatização, independentemente do método utilizado, deve ser realizada em até 20 dias após a colheita, nas condições de AR aqui testadas. Nessas condições, foi possível manter a qualidade sensorial das bananas por até 3 dias após a retirada dos frutos do armazenamento refrigerado.The aim of this study was to evaluate the use of climatization on standardization of 'Prata-Anã' bananas cultivated in Boa Vista, Roraima. After harvested, fruits were selected in the shape of bouquets, sanitized, conditioned by muffling with plastic sheeting or by immersion in a Ethrel® solution, packed with low density polyethylene and stored for four time periods (0, 10, 20 and 30 days at 12 ± 1 °C and 93 ± 2% RH. After each period of cold storage (CS, the fruits were taken

  18. Facial Feature Extraction Method Based on Coefficients of Variances

    Institute of Scientific and Technical Information of China (English)

    Feng-Xi Song; David Zhang; Cai-Kou Chen; Jing-Yu Yang

    2007-01-01

    Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) are two popular feature ex- traction techniques in statistical pattern recognition field. Due to small sample size problem LDA cannot be directly applied to appearance-based face recognition tasks. As a consequence, a lot of LDA-based facial feature extraction techniques are proposed to deal with the problem one after the other. Nullspace Method is one of the most effective methods among them. The Nullspace Method tries to find a set of discriminant vectors which maximize the between-class scatter in the null space of the within-class scatter matrix. The calculation of its discriminant vectors will involve performing singular value decomposition on a high-dimensional matrix. It is generally memory- and time-consuming. Borrowing the key idea in Nullspace method and the concept of coefficient of variance in statistical analysis we present a novel facial feature extraction method, i.e., Discriminant based on Coefficient of Variance (DCV) in this paper. Experimental results performed on the FERET and AR face image databases demonstrate that DCV is a promising technique in comparison with Eigenfaces, Nullspace Method, and other state-of-the-art facial feature extraction methods.

  19. Predictive mapping of soil organic carbon in wet cultivated lands using classification-tree based models: the case study of Denmark.

    Science.gov (United States)

    Bou Kheir, Rania; Greve, Mogens H; Bøcher, Peder K; Greve, Mette B; Larsen, René; McCloy, Keith

    2010-05-01

    Soil organic carbon (SOC) is one of the most important carbon stocks globally and has large potential to affect global climate. Distribution patterns of SOC in Denmark constitute a nation-wide baseline for studies on soil carbon changes (with respect to Kyoto protocol). This paper predicts and maps the geographic distribution of SOC across Denmark using remote sensing (RS), geographic information systems (GISs) and decision-tree modeling (un-pruned and pruned classification trees). Seventeen parameters, i.e. parent material, soil type, landscape type, elevation, slope gradient, slope aspect, mean curvature, plan curvature, profile curvature, flow accumulation, specific catchment area, tangent slope, tangent curvature, steady-state wetness index, Normalized Difference Vegetation Index (NDVI), Normalized Difference Wetness Index (NDWI) and Soil Color Index (SCI) were generated to statistically explain SOC field measurements in the area of interest (Denmark). A large number of tree-based classification models (588) were developed using (i) all of the parameters, (ii) all Digital Elevation Model (DEM) parameters only, (iii) the primary DEM parameters only, (iv), the remote sensing (RS) indices only, (v) selected pairs of parameters, (vi) soil type, parent material and landscape type only, and (vii) the parameters having a high impact on SOC distribution in built pruned trees. The best constructed classification tree models (in the number of three) with the lowest misclassification error (ME) and the lowest number of nodes (N) as well are: (i) the tree (T1) combining all of the parameters (ME=29.5%; N=54); (ii) the tree (T2) based on the parent material, soil type and landscape type (ME=31.5%; N=14); and (iii) the tree (T3) constructed using parent material, soil type, landscape type, elevation, tangent slope and SCI (ME=30%; N=39). The produced SOC maps at 1:50,000 cartographic scale using these trees are highly matching with coincidence values equal to 90.5% (Map T1

  20. Testability integrated evaluation method based on testability virtual test data

    Institute of Scientific and Technical Information of China (English)

    Liu Guanjun; Zhao Chenxu; Qiu Jing; Zhang Yong

    2014-01-01

    Testability virtual test is a new test method for testability verification, which has the advantages such as low cost, few restrictions and large sample of test data. It can be used to make up the deficiency of testability physical test. In order to take the advantage of testability virtual test data effectively and to improve the accuracy of testability evaluation, a testability integrated eval-uation method is proposed in this paper based on testability virtual test data. Considering the char-acteristic of testability virtual test data, the credibility analysis method for testability virtual test data is studied firstly. Then the integrated calculation method is proposed fusing the testability vir-tual and physical test data. Finally, certain helicopter heading and attitude system is presented to demonstrate the proposed method. The results show that the testability integrated evaluation method is feasible and effective.

  1. Preconditioned WR-LMF-based method for ODE systems

    Science.gov (United States)

    Jin, Xiao-Qing; Sin, Vai-Kuong; Song, Li-Li

    2004-01-01

    The waveform relaxation (WR) method was developed as an iterative method for solving large systems of ordinary differential equations (ODEs). In each WR iteration, we are required to solve a system of ODEs. We then introduce the boundary value method (BVM) which is a relatively new method based on the linear multistep formulae to solve ODEs. In particular, we apply the generalized minimal residual method with the Strang-type block-circulant preconditioner for solving linear systems arising from the application of BVMs to each WR iteration. It is demonstrated that these techniques are very effective in speeding up the convergence rate of the resulting iterative processes. Numerical experiments are presented to illustrate the effectiveness of our methods.

  2. Testability integrated evaluation method based on testability virtual test data

    Directory of Open Access Journals (Sweden)

    Liu Guanjun

    2014-02-01

    Full Text Available Testability virtual test is a new test method for testability verification, which has the advantages such as low cost, few restrictions and large sample of test data. It can be used to make up the deficiency of testability physical test. In order to take the advantage of testability virtual test data effectively and to improve the accuracy of testability evaluation, a testability integrated evaluation method is proposed in this paper based on testability virtual test data. Considering the characteristic of testability virtual test data, the credibility analysis method for testability virtual test data is studied firstly. Then the integrated calculation method is proposed fusing the testability virtual and physical test data. Finally, certain helicopter heading and attitude system is presented to demonstrate the proposed method. The results show that the testability integrated evaluation method is feasible and effective.

  3. Quaternion-based discriminant analysis method for color face recognition.

    Science.gov (United States)

    Xu, Yong

    2012-01-01

    Pattern recognition techniques have been used to automatically recognize the objects, personal identities, predict the function of protein, the category of the cancer, identify lesion, perform product inspection, and so on. In this paper we propose a novel quaternion-based discriminant method. This method represents and classifies color images in a simple and mathematically tractable way. The proposed method is suitable for a large variety of real-world applications such as color face recognition and classification of the ground target shown in multispectrum remote images. This method first uses the quaternion number to denote the pixel in the color image and exploits a quaternion vector to represent the color image. This method then uses the linear discriminant analysis algorithm to transform the quaternion vector into a lower-dimensional quaternion vector and classifies it in this space. The experimental results show that the proposed method can obtain a very high accuracy for color face recognition. PMID:22937054

  4. A SUBARRAY-SYNTHESIS BASED 2D DOA ESTIMATION METHOD

    Institute of Scientific and Technical Information of China (English)

    Xu Wenlong; Jiang Wei; Li Zengfu; Shang Yong; Xiang Haige

    2006-01-01

    In some satellite communications, we need to perform Direction Of Arrival (DOA) angle estimation under the restriction that the number of receivers is less than that of the array elements in an array antenna.To solve the conundrum, a method named subarray-synthesis-based Two-Dimensional DOA (2D DOA) angle estimation is proposed. In the method, firstly, the array antenna is divided into a series of subarray antennas based on the total number of receivers; secondly, the subarray antennas' output covariance matrices are estimated; thirdly, an equivalent covariance matrix is synthesized based on the subarray output covariance matrices; then 2D DOA estimation is performed. Monte Carlo simulations showed that the estimation method is effective.

  5. Quantitative trait locus analysis and construction of consensus genetic map for drought tolerance traits based on three recombinant inbred line populations in cultivated groundnut (Arachis hypogaea L.).

    Science.gov (United States)

    Gautami, B; Pandey, M K; Vadez, V; Nigam, S N; Ratnakumar, P; Krishnamurthy, L; Radhakrishnan, T; Gowda, M V C; Narasu, M L; Hoisington, D A; Knapp, S J; Varshney, R K

    2012-08-01

    Groundnut (Arachis hypogaea L.) is an important food and cash crop grown mainly in semi-arid tropics (SAT) regions of the world where drought is the major constraint on productivity. With the aim of understanding the genetic basis and identification of quantitative trait loci (QTL) for drought tolerance, two new recombinant inbred line (RIL) mapping populations, namely ICGS 76 × CSMG 84-1 (RIL-2) and ICGS 44 × ICGS 76 (RIL-3), were used. After screening of 3,215 simple sequence repeat (SSR) markers on the parental genotypes of these populations, two new genetic maps were developed with 119 (RIL-2) and 82 (RIL-3) SSR loci. Together with these maps and the reference map with 191 SSR loci based on TAG 24 × ICGV 86031 (RIL-1), a consensus map was constructed with 293 SSR loci distributed over 20 linkage groups, spanning 2,840.8 cM. As all these three populations segregate for drought-tolerance-related traits, a comprehensive QTL analysis identified 153 main effect QTL (M-QTL) and 25 epistatic QTL (E-QTL) for drought-tolerance-related traits. Localization of these QTL on the consensus map provided 16 genomic regions that contained 125 QTL. A few key genomic regions were selected on the basis of the QTL identified in each region, and their expected role in drought adaptation is also discussed. Given that no major QTL for drought adaptation were identified, novel breeding approaches such as marker-assisted recurrent selection (MARS) and genomic selection (GS) approaches are likely to be the preferred approaches for introgression of a larger number of QTL in order to breed drought-tolerant groundnut genotypes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-011-9660-0) contains supplementary material, which is available to authorized users. PMID:22924017

  6. Image mosaic method based on SIFT features of line segment.

    Science.gov (United States)

    Zhu, Jun; Ren, Mingwu

    2014-01-01

    This paper proposes a novel image mosaic method based on SIFT (Scale Invariant Feature Transform) feature of line segment, aiming to resolve incident scaling, rotation, changes in lighting condition, and so on between two images in the panoramic image mosaic process. This method firstly uses Harris corner detection operator to detect key points. Secondly, it constructs directed line segments, describes them with SIFT feature, and matches those directed segments to acquire rough point matching. Finally, Ransac method is used to eliminate wrong pairs in order to accomplish image mosaic. The results from experiment based on four pairs of images show that our method has strong robustness for resolution, lighting, rotation, and scaling. PMID:24511326

  7. Image Mosaic Method Based on SIFT Features of Line Segment

    Directory of Open Access Journals (Sweden)

    Jun Zhu

    2014-01-01

    Full Text Available This paper proposes a novel image mosaic method based on SIFT (Scale Invariant Feature Transform feature of line segment, aiming to resolve incident scaling, rotation, changes in lighting condition, and so on between two images in the panoramic image mosaic process. This method firstly uses Harris corner detection operator to detect key points. Secondly, it constructs directed line segments, describes them with SIFT feature, and matches those directed segments to acquire rough point matching. Finally, Ransac method is used to eliminate wrong pairs in order to accomplish image mosaic. The results from experiment based on four pairs of images show that our method has strong robustness for resolution, lighting, rotation, and scaling.

  8. Analysis of Dynamic Modeling Method Based on Boundary Element

    Directory of Open Access Journals (Sweden)

    Xu-Sheng Gan

    2013-07-01

    Full Text Available The aim of this study was to study an improved dynamic modeling method based on a Boundary Element Method (BEM. The dynamic model was composed of the elements such as the beam element, plate element, joint element, lumped mass and spring element by the BEM. An improved dynamic model of a machine structure was established based on plate-beam element system mainly. As a result, the dynamic characteristics of a machine structure were analyzed and the comparison of computational results and experimental’s showed the modeling method was effective. The analyses indicate that the introduced method inaugurates a good way for analyzing dynamic characteristics of a machine structure efficiently.

  9. Local coding based matching kernel method for image classification.

    Science.gov (United States)

    Song, Yan; McLoughlin, Ian Vince; Dai, Li-Rong

    2014-01-01

    This paper mainly focuses on how to effectively and efficiently measure visual similarity for local feature based representation. Among existing methods, metrics based on Bag of Visual Word (BoV) techniques are efficient and conceptually simple, at the expense of effectiveness. By contrast, kernel based metrics are more effective, but at the cost of greater computational complexity and increased storage requirements. We show that a unified visual matching framework can be developed to encompass both BoV and kernel based metrics, in which local kernel plays an important role between feature pairs or between features and their reconstruction. Generally, local kernels are defined using Euclidean distance or its derivatives, based either explicitly or implicitly on an assumption of Gaussian noise. However, local features such as SIFT and HoG often follow a heavy-tailed distribution which tends to undermine the motivation behind Euclidean metrics. Motivated by recent advances in feature coding techniques, a novel efficient local coding based matching kernel (LCMK) method is proposed. This exploits the manifold structures in Hilbert space derived from local kernels. The proposed method combines advantages of both BoV and kernel based metrics, and achieves a linear computational complexity. This enables efficient and scalable visual matching to be performed on large scale image sets. To evaluate the effectiveness of the proposed LCMK method, we conduct extensive experiments with widely used benchmark datasets, including 15-Scenes, Caltech101/256, PASCAL VOC 2007 and 2011 datasets. Experimental results confirm the effectiveness of the relatively efficient LCMK method. PMID:25119982

  10. Local coding based matching kernel method for image classification.

    Directory of Open Access Journals (Sweden)

    Yan Song

    Full Text Available This paper mainly focuses on how to effectively and efficiently measure visual similarity for local feature based representation. Among existing methods, metrics based on Bag of Visual Word (BoV techniques are efficient and conceptually simple, at the expense of effectiveness. By contrast, kernel based metrics are more effective, but at the cost of greater computational complexity and increased storage requirements. We show that a unified visual matching framework can be developed to encompass both BoV and kernel based metrics, in which local kernel plays an important role between feature pairs or between features and their reconstruction. Generally, local kernels are defined using Euclidean distance or its derivatives, based either explicitly or implicitly on an assumption of Gaussian noise. However, local features such as SIFT and HoG often follow a heavy-tailed distribution which tends to undermine the motivation behind Euclidean metrics. Motivated by recent advances in feature coding techniques, a novel efficient local coding based matching kernel (LCMK method is proposed. This exploits the manifold structures in Hilbert space derived from local kernels. The proposed method combines advantages of both BoV and kernel based metrics, and achieves a linear computational complexity. This enables efficient and scalable visual matching to be performed on large scale image sets. To evaluate the effectiveness of the proposed LCMK method, we conduct extensive experiments with widely used benchmark datasets, including 15-Scenes, Caltech101/256, PASCAL VOC 2007 and 2011 datasets. Experimental results confirm the effectiveness of the relatively efficient LCMK method.

  11. XML-based product information processing method for product design

    Science.gov (United States)

    Zhang, Zhen Yu

    2012-01-01

    Design knowledge of modern mechatronics product is based on information processing as the center of the knowledge-intensive engineering, thus product design innovation is essentially the knowledge and information processing innovation. Analysis of the role of mechatronics product design knowledge and information management features, a unified model of XML-based product information processing method is proposed. Information processing model of product design includes functional knowledge, structural knowledge and their relationships. For the expression of product function element, product structure element, product mapping relationship between function and structure based on the XML model are proposed. The information processing of a parallel friction roller is given as an example, which demonstrates that this method is obviously helpful for knowledge-based design system and product innovation.

  12. Wavelet basis construction method based on separation blast vibration signal

    Institute of Scientific and Technical Information of China (English)

    凌同华; 张胜; 陈倩倩; 李洁

    2015-01-01

    As wavelet basis in wavelet analysis is neither arbitrary nor unique, the same signal dealing with different wavelet bases will generate different results. Therefore, how to construct a wavelet basis suitable for the characteristics of the analyzed signal and solve its algorithm and realization is a fundamental problem which perplexed many researchers. To solve these problems, in accordance with the basic features of the measured millisecond blast vibration signal, a new wavelet basis construction method based on the separation blast vibration signal is proposed, and the feasibility of this method is verified by comparing the practical effect of the newly constructed wavelet with other known wavelets in signal processing.

  13. CONSTRUCTION METHOD OF KNOWLEDGE MAP BASED ON DESIGN PROCESS

    Institute of Scientific and Technical Information of China (English)

    SU Hai; JIANG Zuhua

    2007-01-01

    Due to the increasing amount and complexity of knowledge in product design, the knowledge map based on design process is presented as a tool to reuse product design process, promote the product design knowledge sharing. The relationship between design task flow and knowledge flow is discussed; A knowledge organizing method based on design task decomposition and a visualization method to support the knowledge retrieving and sharing in product design are proposed. And a knowledge map system to manage the knowledge in product design process is built with Visual C++ and SVG. Finally, a brief case study is provided to illustrate the construction and application of knowledge map in fuel pump design.

  14. A Novel Spectrum Handoff Method Based On Spectrum Reservation

    Directory of Open Access Journals (Sweden)

    Zheng Yong

    2013-07-01

    Full Text Available In this paper, we apply fuzzy analytic hierarchy process (FAHP method in the decision process of Cognitive Radio Networks (CRN spectrum handoff. Based on the pre-determined target spectrum list model, considering the multiple indicators which influence the handoff performance, we designed a spectrum handoff method based on spectrum reservation strategy. Simulation results show that the algorithm proposed in this paper exceeds the random handoff algorithm without channel order, it can significantly reduce the handoff frequency and time overhead of cognitive users, reduce the system delay and improve the system throughput.

  15. An Image Encryption Method Based on Bit Plane Hiding Technology

    Institute of Scientific and Technical Information of China (English)

    LIU Bin; LI Zhitang; TU Hao

    2006-01-01

    A novel image hiding method based on the correlation analysis of bit plane is described in this paper. Firstly, based on the correlation analysis, different bit plane of a secret image is hided in different bit plane of several different open images. And then a new hiding image is acquired by a nesting "Exclusive-OR" operation on those images obtained from the first step. At last, by employing image fusion technique, the final hiding result is achieved. The experimental result shows that the method proposed in this paper is effective.

  16. IDEF method-based simulation model design and development framework

    Directory of Open Access Journals (Sweden)

    Ki-Young Jeong

    2009-09-01

    Full Text Available The purpose of this study is to provide an IDEF method-based integrated framework for a business process simulation model to reduce the model development time by increasing the communication and knowledge reusability during a simulation project. In this framework, simulation requirements are collected by a function modeling method (IDEF0 and a process modeling method (IDEF3. Based on these requirements, a common data model is constructed using the IDEF1X method. From this reusable data model, multiple simulation models are automatically generated using a database-driven simulation model development approach. The framework is claimed to help both requirement collection and experimentation phases during a simulation project by improving system knowledge, model reusability, and maintainability through the systematic use of three descriptive IDEF methods and the features of the relational database technologies. A complex semiconductor fabrication case study was used as a testbed to evaluate and illustrate the concepts and the framework. Two different simulation software products were used to develop and control the semiconductor model from the same knowledge base. The case study empirically showed that this framework could help improve the simulation project processes by using IDEF-based descriptive models and the relational database technology. Authors also concluded that this framework could be easily applied to other analytical model generation by separating the logic from the data.

  17. A single scale retinex based method for palm vein extraction

    OpenAIRE

    Wang, Chongyang; Peng, Ming; Xu, Lingfeng; Chen, Tong

    2016-01-01

    Palm vein recognition is a novel biometric identification technology. But how to gain a better vein extraction result from the raw palm image is still a challenging problem, especially when the raw data collection has the problem of asymmetric illumination. This paper proposes a method based on single scale Retinex algorithm to extract palm vein image when strong shadow presents due to asymmetric illumination and uneven geometry of the palm. We test our method on a multispectral palm image. T...

  18. New de-interlacing method based on adaptive weight

    Institute of Scientific and Technical Information of China (English)

    赵建伟; 古雪丰; 王朋; 刘重庆

    2004-01-01

    De-interlacing is very important when converting interlaced pictures to progressive pictures in format conversion.Multi-formats digital broadcast and progressive display requires the de-interlacing technique. An adaptive weight deinterlacing method is proposed. It combines motion compensation technique with directional-based spatio-temporal filter efficiently. Experiment results indicate that the method can keep edge continuity and sharpness effectively, reduce the artifacts in motion areas, and shows better visual performance when the estimated motion vectors are inaccurate.

  19. Validation of some FM-based fitness for purpose methods

    International Nuclear Information System (INIS)

    The reliability of several FM-based fitness-for-purpose methods has been investigated on a number of objects for which accurate fracture data were available from experiments or from practice, viz. 23 wide plates, 30 mm thickness (surface and through thickness cracks, cracks at holes, with and without welds), 45 pipelines sections with cracks, pressure vessels and a T-joint. The methods applied mainly comprise ASME XI, PD 6493 and R6. This contribution reviews the results. (author)

  20. NUMERICAL METHODS FOR DIFFERENTIAL GAMES BASED ON PARTIAL DIFFERENTIAL EQUATIONS

    OpenAIRE

    Falcone, M

    2006-01-01

    In this paper we present some numerical methods for the solution of two-persons zero-sum deterministic differential games. The methods are based on the dynamic programming approach. We first solve the Isaacs equation associated to the game to get an approximate value function and then we use it to reconstruct approximate optimal feedback controls and optimal trajectories. The approximation schemes also have an interesting control interpretation since the time-discrete scheme stems from a dyna...

  1. A Ranking Method of Retrieval Results Based on Web Comprehending

    OpenAIRE

    Zhijuan Deng; Shaojun Zhong

    2011-01-01

    This thesis put forward a method used to calculate query similarity of webpage search results based on Web comprehending. According to users’ query input, this method can use Web comprehending technology to display the important web pages closer to users’ query in the first page of the list, make users more satisfied with the response of search engine, running after recall ratio and ensure precision at the same time.

  2. A questionnaire based evaluation of teaching methods amongst MBBS students

    OpenAIRE

    Muneshwar JN, Mirza Shiraz Baig, Zingade US, Khan ST

    2013-01-01

    The medical education and health care in India are facing serious challenges in content and competencies. Heightened focus on the quality of teaching in medical college has led to increased use of student surveys as a means of evaluating teaching. Objectives: A questionnaire based evaluation of 200 students (I MBBS & II MBBS) about teaching methods was conducted at a Govt Medical College & Hospital, Aurangabad (MS) with intake capacity of 150 students &established since 50 last years. Methods...

  3. Progress of DNA-based Methods for Species Identification

    Institute of Scientific and Technical Information of China (English)

    HU Zhen; ZHANG Su-hua; WANG Zheng; BIAN Ying-nan; LI Cheng-tao

    2015-01-01

    Species identification of biological samples is widely used in such fields as forensic science and food industry. A variety of accurate and reliable methods have been developed in recent years. The cur-rent reviewshows common target genes and screening criteria suitable for species identification, and de-scribed various DNA-based molecular biology methods about species identification. Additionally, it dis-cusses the future development of species identification combined with real-time PCR and sequencing technologies.

  4. NETS FOR PEACH PROTECTED CULTIVATION

    Directory of Open Access Journals (Sweden)

    Evelia Schettini

    2012-06-01

    Full Text Available The aim of this paper was to investigate the radiometric properties of coloured nets used to protect a peach cultivation. The modifications of the solar spectral distribution, mainly in the R and FR wavelength band, influence plant photomorphogenesis by means of the phytochrome and cryptochrome. The phytochrome response is characterized in terms of radiation rate in the red wavelengths (R, 600-700 nm to that in the farred radiation (FR, 700-800 nm, i.e. the R/FR ratio. The effects of the blue radiation (B, 400-500 nm is investigated by the ratio between the blue radiation and the far-red radiation, i.e. the B/FR ratio. A BLUE net, a RED net, a YELLOW net, a PEARL net, a GREY net and a NEUTRAL net were tested in Bari (Italy, latitude 41° 05’ N. Peach trees were located in pots inside the greenhouses and in open field. The growth of the trees cultivated in open field was lower in comparison to the growth of the trees grown under the nets. The RED, PEARL, YELLOW and GREY nets increased the growth of the trees more than the other nets. The nets positively influenced the fruit characteristics, such as fruit weight and flesh firmness.

  5. On spectral methods for variance based sensitivity analysis

    OpenAIRE

    Alexanderian, Alen

    2013-01-01

    Consider a mathematical model with a finite number of random parameters. Variance based sensitivity analysis provides a framework to characterize the contribution of the individual parameters to the total variance of the model response. We consider the spectral methods for variance based sensitivity analysis which utilize representations of square integrable random variables in a generalized polynomial chaos basis. Taking a measure theoretic point of view, we provide a rigorous and at the sam...

  6. Model Based Bootstrap Methods for Interval Censored Data

    OpenAIRE

    Sen, Bodhisattva; Xu, Gongjun

    2013-01-01

    We investigate the performance of model based bootstrap methods for constructing point-wise confidence intervals around the survival function with interval censored data. We show that bootstrapping from the nonparametric maximum likelihood estimator of the survival function is inconsistent for both the current status and case 2 interval censoring models. A model based smoothed bootstrap procedure is proposed and shown to be consistent. In addition, simulation studies are conducted to illustra...

  7. AN IMPROVED GRAPH BASED METHOD FOR EXTRACTING ASSOCIATION RULES

    OpenAIRE

    Wael AlZoubi

    2015-01-01

    This paper proposes an improved approach to mine strong association rules from an association graph, called graph based association rule mining (GBAR) method, where the association for each frequent itemset is represented by a sub-graph, then all sub-graphs are merged to determine association rules with high confidence and eliminate weak rules, the proposed graph based technique is self-motivated since it builds the association graph in a successive manner. These rules achieve the scalability...

  8. Gamification as an Intervention Method in Practice-Based Innovation

    OpenAIRE

    Mirva Hyypiä; Satu Parjanen

    2015-01-01

    This paper concentrates on the possibilities offered by gamification in practice-based innovation activities. It addresses the following research questions: How does gamification enhance creativity in practice-based innovation? How can gamification be modified into a method that facilitates experiences of gamefulness? A case study presented in the paper examines the impact of gamification in co-creating a value-adding network for open innovation processes between organizations. The results in...

  9. Improving merge methods for grid-based digital elevation models

    Science.gov (United States)

    Leitão, J. P.; Prodanović, D.; Maksimović, Č.

    2016-03-01

    Digital Elevation Models (DEMs) are used to represent the terrain in applications such as, for example, overland flow modelling or viewshed analysis. DEMs generated from digitising contour lines or obtained by LiDAR or satellite data are now widely available. However, in some cases, the area of study is covered by more than one of the available elevation data sets. In these cases the relevant DEMs may need to be merged. The merged DEM must retain the most accurate elevation information available while generating consistent slopes and aspects. In this paper we present a thorough analysis of three conventional grid-based DEM merging methods that are available in commercial GIS software. These methods are evaluated for their applicability in merging DEMs and, based on evaluation results, a method for improving the merging of grid-based DEMs is proposed. DEMs generated by the proposed method, called MBlend, showed significant improvements when compared to DEMs produced by the three conventional methods in terms of elevation, slope and aspect accuracy, ensuring also smooth elevation transitions between the original DEMs. The results produced by the improved method are highly relevant different applications in terrain analysis, e.g., visibility, or spotting irregularities in landforms and for modelling terrain phenomena, such as overland flow.

  10. Possibilities of Strawberry Integrated Disease Management in Different Cultivation System

    Directory of Open Access Journals (Sweden)

    Tihomir Miličević

    2006-01-01

    Full Text Available In the last few years strawberry production in Croatia is constantly increasing. One of the main problems in production are diseases. During two-year trials in strawberry plantations in northern Croatia, the occurrence of diseases was monitored in order to establish the most effective methods of integrated disease management. Trials were performed in three cultivation system: open field, greenhouse and hydroponics. The most frequent disease in all three production systems was gray mould (Botrytis cinerea. In open field production, the occurrence of common leaf spot (Mycosphaerella fragariae and leaf scorch (Diplocarpon earliana were also frequently observed, while leaf blotch (Gnomonia comari, leaf blight (Phomopsis obscurans and fruit anthracnose (Colletotrichum spp. were only sporadically present. For the control of the most important disease, gray mould, forecast model BOTMAN was implemented. As relatively simple model based on meteorological data, BOTMAN allowed effective, ecologically and economically more acceptable control, based on integrated chemical and biological measures. Meteorological data were obtained from the State Hydrometeorological Department (DHMZ. Results showed no significant difference in intensity of gray mould infection between usual chemical control and BOTMAN-based control. Two-years research on strawberry disease management in Croatia revealed perspective possibilities of integrated strawberry disease management.

  11. Possibilities of Strawberry Integrated Disease Management in Different Cultivation System

    Directory of Open Access Journals (Sweden)

    Tihomir Miličević

    2006-12-01

    Full Text Available In the last few years strawberry production in Croatia is constantly increasing. One of the main problems in production are diseases. During two-year trials in strawberry plantations in northern Croatia, the occurrence of diseases was monitored in order to establish the most effective methods of integrated disease management. Trials were performed in three cultivation system: open field, greenhouse and hydroponics. The most frequent disease in all three production systems was gray mould (Botrytis cinerea. In open field production, the occurrence of common leaf spot (Mycosphaerella fragariae and leaf scorch (Diplocarpon earliana were also frequently observed, while leaf blotch (Gnomonia comari, leaf blight (Phomopsis obscurans and fruit anthracnose (Colletotrichum spp. were only sporadically present. For the control of the most important disease, gray mould, forecast model BOTMAN was implemented. As relatively simple model based on meteorological data, BOTMAN allowed effective, ecologically and economically more acceptable control, based on integrated chemical and biological measures. Meteorological data were obtained from the State Hydrometeorological Department (DHMZ. Results showed no significant difference in intensity of gray mould infection between usual chemical control and BOTMAN-based control. Two-years research on strawberry disease management in Croatia revealed perspective possibilities of integrated strawberry disease management.

  12. Much Improved Water Use Efficiency of Rice under Non-Flooded Mulching Cultivation

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Water shortage is increasingly limiting the luxury use of water in rice cultivation. In this study, non-flooded mulching cultivation of rice only consumed a fraction of the water that was needed for traditional flooded cultivation and largely maintained the grain yield. We also investigated the growth and development of rice plants and examined grain yield formation when rice was subjected to non-flooded mulching cultivation. One indica hybrid rice combination was grown in a field experiment and three cultivation methods, traditional flooding (TF), non-flooded straw mulching cultivation (SM) and non-flooded plastic mulching cultivation (PM), were conducted during the whole season. Grain yield showed that there was no significant difference between SM and TF rice, but the grain yield of SM cultivation was significantly higher than that of PM. The tiller numbers were inhibited in the early stage under non-flooded mulching cultivation, but the situation was reversed at the later period. Both SM and PM rice reduced dry matter accumulation of shoot, but increased root dry weight,enhanced the remobilization of assimilates from stems to grains and increased the harvest index. During the middle and later grain filling period, mulched plants showed a faster decrease in chlorophyll concentrations, photosynthetic rates of flag leaves and root activity than TF rice, indicating that non-flooded mulching cultivation enhanced plant senescence. In comparison, SM treatment produced higher grain yield and, more dry matter accumulation and panicle numbers than the PM treatment. The overall results suggest that high yield of non-flooded mulching cultivation of rice can be achieved with much improved irrigational water use efficiency.

  13. EFFICIENCY OF FERTILIZATION AND SOIL CULTIVATION IN CROP ROTATION

    Directory of Open Access Journals (Sweden)

    Eva CANDRÁKOVÁ

    2009-03-01

    Full Text Available The experiment with the crop rotation: winter wheat, pea, corn, spring barley and cow-grass were founded in 2001- 2004. We examined the effect of the preceding crop, the soil cultivation and fertilization on yield of grain of the main product. The methods of soil cultivation: tillage to the depth of 0.25 m, to the depth of 0.15 m and cultivation where we used disk tools to the depth of 0.10 m. In the variants of fertilization had been used fertilization of the artificial fertilizer with the balance method and fertilization with the artificial fertilizer with the placement of the remains of the preceding crop. By grain corn were statistically significant yield achieved after the tillage to the depth of 0.25 m and 0.15 m. Spring barley reacted positive on the soil cultivation with disk tools. Yield of the winter wheat and the pea were not statistically significant. Fertilization affected statistically significant on yield of winter wheat. The effect of the artificial fertilizers and the residues of the preceding crop on yield of corn, spring barley and pea did not show statistically significant.

  14. A Star Pattern Recognition Method Based on Decreasing Redundancy Matching

    Science.gov (United States)

    Yao, Lu; Xiao-xiang, Zhang; Rong-yu, Sun

    2016-04-01

    During the optical observation of space objects, it is difficult to enable the background stars to get matched when the telescope pointing error and tracking error are significant. Based on the idea of decreasing redundancy matching, an effective recognition method for background stars is proposed in this paper. The simulative images under different conditions and the observed images are used to verify the proposed method. The experimental results show that the proposed method has raised the rate of recognition and reduced the time consumption, it can be used to match star patterns accurately and rapidly.

  15. A GPS-Based Decentralized Control Method for Islanded Microgrids

    DEFF Research Database (Denmark)

    Golsorkhi, Mohammad; Lu, Dylan; Guerrero, Josep M.

    2016-01-01

    sharing of active (id) and reactive (iq) components of current are achieved based on vd-id and vq-iq droop characteristics. The method has been tested on laboratory scale MG. Experimental results demonstrate the efficacy of the proposed method in terms of dynamics, power quality and robustness with......Coordinated control of distributed energy resources (DER) is essential for the operation of islanded microgrids (MGs). Conventionally, such coordination is achieved by drooping the frequency of the reference voltage versus active (or reactive) power. The conventional droop method ensures...

  16. A online credit evaluation method based on AHP and SPA

    Science.gov (United States)

    Xu, Yingtao; Zhang, Ying

    2009-07-01

    Online credit evaluation is the foundation for the establishment of trust and for the management of risk between buyers and sellers in e-commerce. In this paper, a new credit evaluation method based on the analytic hierarchy process (AHP) and the set pair analysis (SPA) is presented to determine the credibility of the electronic commerce participants. It solves some of the drawbacks found in classical credit evaluation methods and broadens the scope of current approaches. Both qualitative and quantitative indicators are considered in the proposed method, then a overall credit score is achieved from the optimal perspective. In the end, a case analysis of China Garment Network is provided for illustrative purposes.

  17. An AIS-Based E-mail Classification Method

    Science.gov (United States)

    Qing, Jinjian; Mao, Ruilong; Bie, Rongfang; Gao, Xiao-Zhi

    This paper proposes a new e-mail classification method based on the Artificial Immune System (AIS), which is endowed with good diversity and self-adaptive ability by using the immune learning, immune memory, and immune recognition. In our method, the features of spam and non-spam extracted from the training sets are combined together, and the number of false positives (non-spam messages that are incorrectly classified as spam) can be reduced. The experimental results demonstrate that this method is effective in reducing the false rate.

  18. General transmission method based on PXI platform for physical experiments

    International Nuclear Information System (INIS)

    In this paper, a general method of data transmission system design on PXI platform is proposed. It can be used in readout system design for physical experiments. It aims at providing reusable and general interfaces for customized design of PXI while maintaining the transmission performance. It has three main features: (1) universal logic hardware interface, (2) ethernet based socket software interface, and (3) specific and simple data transmission protocol. Data transmission on PXI bus can be realized with the said two universal interfaces coordinated by this specific protocol. Test shows that this method is feasible and stable. This method can be easily reused in readout system designs for different experiments. (authors)

  19. Sonoclot(®)-based method to detect iron enhanced coagulation.

    Science.gov (United States)

    Nielsen, Vance G; Henderson, Jon

    2016-07-01

    Thrombelastographic methods have been recently introduced to detect iron mediated hypercoagulability in settings such as sickle cell disease, hemodialysis, mechanical circulatory support, and neuroinflammation. However, these inflammatory situations may have heme oxygenase-derived, coexistent carbon monoxide present, which also enhances coagulation as assessed by the same thrombelastographic variables that are affected by iron. This brief report presents a novel, Sonoclot-based method to detect iron enhanced coagulation that is independent of carbon monoxide influence. Future investigation will be required to assess the sensitivity of this new method to detect iron mediated hypercoagulability in clinical settings compared to results obtained with thrombelastographic techniques. PMID:26497986

  20. A Design Method of Business Application Framework Based on

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    This paper discusses design and implementation method of BusinessAppl ication Framework based on software patterns, and then presents MVC pattern of a rchitecture and the method of dynamical update promulgation for Business Applica tion Framework. We discuss adaptation of Abstract Factory for the kern el functionality of Business Application Framework, such as data creation, manip ulation, composition, etc. It also presents class model and its class st ructure of Abstract Factory pattern. Finally, we briefly discuss the update, mod ification, and reconstruction method of Business Application Framework.

  1. A Clustering Method Based on the Maximum Entropy Principle

    Directory of Open Access Journals (Sweden)

    Edwin Aldana-Bobadilla

    2015-01-01

    Full Text Available Clustering is an unsupervised process to determine which unlabeled objects in a set share interesting properties. The objects are grouped into k subsets (clusters whose elements optimize a proximity measure. Methods based on information theory have proven to be feasible alternatives. They are based on the assumption that a cluster is one subset with the minimal possible degree of “disorder”. They attempt to minimize the entropy of each cluster. We propose a clustering method based on the maximum entropy principle. Such a method explores the space of all possible probability distributions of the data to find one that maximizes the entropy subject to extra conditions based on prior information about the clusters. The prior information is based on the assumption that the elements of a cluster are “similar” to each other in accordance with some statistical measure. As a consequence of such a principle, those distributions of high entropy that satisfy the conditions are favored over others. Searching the space to find the optimal distribution of object in the clusters represents a hard combinatorial problem, which disallows the use of traditional optimization techniques. Genetic algorithms are a good alternative to solve this problem. We benchmark our method relative to the best theoretical performance, which is given by the Bayes classifier when data are normally distributed, and a multilayer perceptron network, which offers the best practical performance when data are not normal. In general, a supervised classification method will outperform a non-supervised one, since, in the first case, the elements of the classes are known a priori. In what follows, we show that our method’s effectiveness is comparable to a supervised one. This clearly exhibits the superiority of our method.

  2. Comparative study, based on metamodels, of methods for controlling performance

    Directory of Open Access Journals (Sweden)

    Aitouche Samia

    2012-05-01

    Full Text Available The continuing evolution of technology and human behavior puts the company in an uncertain and evolving environment. The company must be responsive and even proactive; therefore, control performance becomes increasingly difficult. Choosing the best method of ensuring control by the management policy of the company and its strategy is also a decision problem. The aim of this paper is the comparative study of three methods: the Balanced Scorecard, GIMSI and SKANDIAs NAVIGATOR for choosing the best method for ensuring the orderly following the policy of the company while maintaining its durability. Our work is divided into three parts. We firstly proposed original structural and kinetic metamodels for the three methods that allow an overall view of a method. Secondly, based on the three metamodels, we have drawn a generic comparison to analyze completeness of the method. Thirdly, we performed a restrictive comparison based on a restrictive set of criteria related to the same aspect example organizational learning, which is one of the bricks of knowledge management for a reconciliation to a proactive organization in an environment disturbed and uncertain, and the urgent needs. We note that we applied the three methods are applied in our precedent works. [1][23

  3. Nonlinear fault diagnosis method based on kernel principal component analysis

    Institute of Scientific and Technical Information of China (English)

    Yan Weiwu; Zhang Chunkai; Shao Huihe

    2005-01-01

    To ensure the system run under working order, detection and diagnosis of faults play an important role in industrial process. This paper proposed a nonlinear fault diagnosis method based on kernel principal component analysis (KPCA). In proposed method, using essential information of nonlinear system extracted by KPCA, we constructed KPCA model of nonlinear system under normal working condition. Then new data were projected onto the KPCA model. When new data are incompatible with the KPCA model, it can be concluded that the nonlinear system isout of normal working condition. Proposed method was applied to fault diagnosison rolling bearings. Simulation results show proposed method provides an effective method for fault detection and diagnosis of nonlinear system.

  4. SET OPERATOR-BASED METHOD OF DENOISING MEDICAL VOLUME DATA

    Institute of Scientific and Technical Information of China (English)

    程兵; 郑南宁; 袁泽剑

    2002-01-01

    Objective To investigate impulsive noise suppression of medical volume data. Methods The volume data is represented as level sets and a special set operator is defined and applied to filtering it. The small connected components, which are likely to be produced by impulsive noise, are eliminated after the filtering process. A fast algorithm that uses a heap data structure is also designed. Results Compared with traditional linear filters such as a Gaussian filter, this method preserves the fine structure features of the medical volume data while removing noise, and the fast algorithm developed by us reduces memory consumption and improves computing efficiency. The experimental results given illustrate the efficiency of the method and the fast algorithm. Conclusion The set operator-based method shows outstanding denoising properties in our experiment, especially for impulsive noise. The method has a wide variety of applications in the areas of volume visualization and high dimensional data processing.

  5. Measurement of unattached radon progeny based in electrostatic deposition method

    International Nuclear Information System (INIS)

    A method for the measurement of unattached radon progeny based on its electrostatic deposition onto wire screens, using only one pump, has been implemented and calibrated. The importance of being able of making use of this method is related with the special radiological significance that has the unattached fraction of the short-lived radon progeny. Because of this, the assessment of exposure could be directly related to dose with far greater accuracy than before. The advantages of this method are its simplicity, even with the tools needed for the sample collection, as well as the measurement instruments used. Also, the suitability of this method is enhanced by the fact that it can effectively be used with a simple measuring procedure such as the Kusnetz method. (author)

  6. A Matrix Pencil Algorithm Based Multiband Iterative Fusion Imaging Method

    Science.gov (United States)

    Zou, Yong Qiang; Gao, Xun Zhang; Li, Xiang; Liu, Yong Xiang

    2016-01-01

    Multiband signal fusion technique is a practicable and efficient way to improve the range resolution of ISAR image. The classical fusion method estimates the poles of each subband signal by the root-MUSIC method, and some good results were get in several experiments. However, this method is fragile in noise for the proper poles could not easy to get in low signal to noise ratio (SNR). In order to eliminate the influence of noise, this paper propose a matrix pencil algorithm based method to estimate the multiband signal poles. And to deal with mutual incoherent between subband signals, the incoherent parameters (ICP) are predicted through the relation of corresponding poles of each subband. Then, an iterative algorithm which aimed to minimize the 2-norm of signal difference is introduced to reduce signal fusion error. Applications to simulate dada verify that the proposed method get better fusion results at low SNR.

  7. Allelopathy as a potential strategy to improve microalgae cultivation.

    Science.gov (United States)

    Bacellar Mendes, Leonardo Brantes; Vermelho, Alane Beatriz

    2013-01-01

    One of the main obstacles for continuous productivity in microalgae cultivation is the presence of biological contaminants capable of eliminating large numbers of cells in a matter of days or even hours. However, a number of strategies are being used to combat and prevent contamination in microalgae cultivation. These strategies include the use of extreme conditions in the culture media such as high salinity and high pH to create an unfavorable environment for the competitive organisms or predators of the microalgae. Numerous studies have explored the potential of naturally occurring bioactive secondary metabolites, which are natural products from plants and microorganisms, as a source of such compounds. Some of these compounds are herbicides, and marine and freshwater microalgae are a source of these compounds. Microalgae produce a remarkable diversity of biologically active metabolites. Results based on the allelopathic potential of algae have only been described for laboratory-scale production and not for algae cultivation on a pilot scale. The adoption of allelopathy on microalgal strains is an unexplored field and may be a novel solution to improve algae production. Here we present information showing the diversity of allelochemicals from microalgae and the use of an allelopathic approach to control microalgae cultivation on a pilot scale based on R&D activities being carried out in Brazil for biodiesel production. PMID:24499580

  8. Effect of Rain-Shelter Cultivation of Vitis vinifera cv. Cabernet Gernischet on the Phenolic Profile of Berry Skins and the Incidence of Grape Diseases

    OpenAIRE

    Teng-Fei Xu; Jiang-Fei Meng; Peng-Fei Ning; Zhen-Wen Zhang

    2012-01-01

    Rain-shelter cultivation is an effective cultural method to prevent rainfall damage during grape harvest and widely applied in the Chinese rainy regions. In this study we investigated the effect of rain-shelter cultivation on grape diseases and phenolic composition in the skins of Vitis vinifera cv. Cabernet Gernischet grape berries through the comparison with open-field cultivation at two vintages (2010 and 2011). The results showed that rain-shelter cultivation reduced the incidence of grap...

  9. A method to manage the model base in DSS

    Institute of Scientific and Technical Information of China (English)

    孙成双; 李桂君

    2004-01-01

    How to manage and use models in DSS is a most important subject. Generally, it costs a lot of money and time to develop the model base management system in the development of DSS and most are simple in function or cannot be used efficiently in practice. It is a very effective, applicable, and economical choice to make use of the interfaces of professional computer software to develop a model base management system. This paper presents the method of using MATLAB, a well-known statistics software, as the development platform of a model base management system. The main functional framework of a MATLAB-based model base managementsystem is discussed. Finally, in this paper, its feasible application is illustrated in the field of construction projects.

  10. 基于能值-生态足迹改进模型的浙江省耕地可持续利用研究%Study on Sustainable Utilization of Cultivated Land in Zhejiang Province Based on Modified Emergy-Ecological Footprint Model

    Institute of Scientific and Technical Information of China (English)

    童悦; 毛传澡; 严力蛟

    2015-01-01

    With sustainable utilization of cultivated land as the entry point, a modified cultivated land ecological footprint model based on energy analysis was used to rectify yield factors of the cultivated land in Zhejiang Province from the angle of “national hectare” and “provincial hectare”. On such a basis, temporal dynamic analysis was performed of sustainable utilization of the cultivated land in Zhejiang during the years of 2003-2012, and spatial differentiation analysis was con⁃ducted of sustainable utilization of the cultivated land in the 11 prefecture⁃level cities of Zhejiang in 2012. Results show that the ecological carrying capacity of cultivated land per⁃capita in Zhejiang during the years of 2003-2012 remained quite low and did not vary much;per capita ecological footprint of cultivated land declined while fluctuating;and the ecological deficit per capita was somewhat mitigated during the ten years, but the cultivated land ecological sustainability index re⁃mained in the range between 0�215 0 and 0�266 4, being at a moderate level of unsustainability. Besides, in 2012, though the 11 cities in Zhejiang Province all incurred large ecological deficits, they varied spatially in per capita ecological carrying capacity and ecological footprint of cultivated land: Ningbo, Zhoushan, Shaoxing, Lishui, Taizhou and Jinhua were rated as moderate in unsustainability while Hangzhou, Wenzhou, Huzhou, Quzhou and Jiaxing were as high in unsus⁃tainability. These findings may provide some theoretical references and solutions for overall prediction and planning of culti⁃vated land ecological security and cultivated land sustainable utilization in Zhejiang Province.%以耕地可持续利用为切入点,应用基于能值分析的耕地生态足迹改进模型,从“国家公顷”和“省公顷”的角度出发修正浙江省耕地产量因子,在此基础上构建耕地生态可持续利用指数,时间维度上对浙江省2003—2012年耕地

  11. Kernel-Based Methods for Hypothesis Testing: A Unified View

    OpenAIRE

    Harchaoui, Zaid; Bach, Francis; Cappe, Olivier; Moulines, Eric

    2013-01-01

    Kernel-based methods provide a rich and elegant framework for developing nonparametric detection procedures for signal processing. Several recently proposed procedures can be simply described using basic concepts of reproducing kernel Hilbert space embeddings of probability distributions, namely mean elements and covariance operators. We propose a unified view of these tools, and draw relationships with information divergences between distributions.

  12. Optimization-based Method for Automated Road Network Extraction

    Energy Technology Data Exchange (ETDEWEB)

    Xiong, D

    2001-09-18

    Automated road information extraction has significant applicability in transportation. It provides a means for creating, maintaining, and updating transportation network databases that are needed for purposes ranging from traffic management to automated vehicle navigation and guidance. This paper is to review literature on the subject of road extraction and to describe a study of an optimization-based method for automated road network extraction.

  13. A Quantum-Based Similarity Method in Virtual Screening

    Directory of Open Access Journals (Sweden)

    Mohammed Mumtaz Al-Dabbagh

    2015-10-01

    Full Text Available One of the most widely-used techniques for ligand-based virtual screening is similarity searching. This study adopted the concepts of quantum mechanics to present as state-of-the-art similarity method of molecules inspired from quantum theory. The representation of molecular compounds in mathematical quantum space plays a vital role in the development of quantum-based similarity approach. One of the key concepts of quantum theory is the use of complex numbers. Hence, this study proposed three various techniques to embed and to re-represent the molecular compounds to correspond with complex numbers format. The quantum-based similarity method that developed in this study depending on complex pure Hilbert space of molecules called Standard Quantum-Based (SQB. The recall of retrieved active molecules were at top 1% and top 5%, and significant test is used to evaluate our proposed methods. The MDL drug data report (MDDR, maximum unbiased validation (MUV and Directory of Useful Decoys (DUD data sets were used for experiments and were represented by 2D fingerprints. Simulated virtual screening experiment show that the effectiveness of SQB method was significantly increased due to the role of representational power of molecular compounds in complex numbers forms compared to Tanimoto benchmark similarity measure.

  14. Agile Service Development: A Rule-Based Method Engineering Approach

    NARCIS (Netherlands)

    Hoppenbrouwers, Stijn; Zoet, Martijn; Versendaal, Johan; Weerd, Inge van de

    2011-01-01

    Agile software development has evolved into an increasingly mature software development approach and has been applied successfully in many software vendors’ development departments. In this position paper, we address the broader agile service development. Based on method engineering principles we de

  15. A Novel Image Fusion Method Based on FRFT-NSCT

    Directory of Open Access Journals (Sweden)

    Peiguang Wang

    2013-01-01

    fused image is obtained by performing the inverse NSCT and inverse FRFT on the combined coefficients. Three modes images and three fusion rules are demonstrated in the proposed algorithm test. The simulation results show that the proposed fusion approach is better than the methods based on NSCT at the same parameters.

  16. A simulation based engineering method to support HAZOP studies

    DEFF Research Database (Denmark)

    Enemark-Rasmussen, Rasmus; Cameron, David; Angelo, Per Bagge;

    2012-01-01

    HAZOP is the most commonly used process hazard analysis tool in industry, a systematic yet tedious and time consuming method. The aim of this study is to explore the feasibility of process dynamic simulations to facilitate the HAZOP studies. We propose a simulation-based methodology to complement...

  17. Optimization-based Method for Automated Road Network Extraction

    International Nuclear Information System (INIS)

    Automated road information extraction has significant applicability in transportation. It provides a means for creating, maintaining, and updating transportation network databases that are needed for purposes ranging from traffic management to automated vehicle navigation and guidance. This paper is to review literature on the subject of road extraction and to describe a study of an optimization-based method for automated road network extraction

  18. Effective Teaching Methods--Project-based Learning in Physics

    Science.gov (United States)

    Holubova, Renata

    2008-01-01

    The paper presents results of the research of new effective teaching methods in physics and science. It is found out that it is necessary to educate pre-service teachers in approaches stressing the importance of the own activity of students, in competences how to create an interdisciplinary project. Project-based physics teaching and learning…

  19. New methods for preparing mercury-based ferrofluids

    DEFF Research Database (Denmark)

    Linderoth, Søren; Rasmussen, L.H.; Mørup, Steen

    1991-01-01

    Metallic ferrofluids based on magnetic particles in mercury have been produced by two new methods. Alloy particles of Fe-B, Fe-Co-B, Fe-Ni-B, and Co-B were prepared by reduction of the transition metal ions in aqueous solutions by NaBH4 and subsequently suspended in mercury. In one preparation, the...

  20. Planning of operation & maintenance using risk and reliability based methods

    DEFF Research Database (Denmark)

    Florian, Mihai; Sørensen, John Dalsgaard

    2015-01-01

    Operation and maintenance (OM) of offshore wind turbines contributes with a substantial part of the total levelized cost of energy (LCOE). The objective of this paper is to present an application of risk- and reliability-based methods for planning of OM. The theoretical basis is presented and...