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Sample records for barley seeds real-time

  1. Barley seed aging

    NARCIS (Netherlands)

    Nagel, Manuela; Kodde, Jan; Pistrick, Sibylle; Mascher, Martin; Börner, Andreas; Groot, Steven P.C.

    2016-01-01

    Experimental seed aging approaches intend to mimic seed deterioration processes to achieve a storage interval reduction. Common methods apply higher seed moisture levels and temperatures. In contrast, the “elevated partial pressure of oxygen” (EPPO) approach treats dry seed stored at ambient temp

  2. Alanine aminotransferase controls seed dormancy in barley

    Science.gov (United States)

    Sato, Kazuhiro; Yamane, Miki; Yamaji, Nami; Kanamori, Hiroyuki; Tagiri, Akemi; Schwerdt, Julian G.; Fincher, Geoffrey B.; Matsumoto, Takashi; Takeda, Kazuyoshi; Komatsuda, Takao

    2016-01-01

    Dormancy allows wild barley grains to survive dry summers in the Near East. After domestication, barley was selected for shorter dormancy periods. Here we isolate the major seed dormancy gene qsd1 from wild barley, which encodes an alanine aminotransferase (AlaAT). The seed dormancy gene is expressed specifically in the embryo. The AlaAT isoenzymes encoded by the long and short dormancy alleles differ in a single amino acid residue. The reduced dormancy allele Qsd1 evolved from barleys that were first domesticated in the southern Levant and had the long dormancy qsd1 allele that can be traced back to wild barleys. The reduced dormancy mutation likely contributed to the enhanced performance of barley in industrial applications such as beer and whisky production, which involve controlled germination. In contrast, the long dormancy allele might be used to control pre-harvest sprouting in higher rainfall areas to enhance global adaptation of barley. PMID:27188711

  3. Barley seed proteomics from spots to structures

    DEFF Research Database (Denmark)

    Finnie, Christine; Svensson, Birte

    2009-01-01

    with information from rice and other cereals facilitate identification of barley proteins. Several hundred barley seed proteins are identified and lower abundance proteins including membrane proteins are now being analysed. In the present review we focus on variation in protein profiles of seed tissues during...... forms on 2D-gels. Specific protein families, including peroxidases and alpha-amylases have been subjected to in-depth analysis resulting in characterisation of different isozymes, post-translational. modifications and processing. A functional proteomics study focusing on the seed thioredoxin system has...

  4. Wheat and barley seed systems in Ethiopia and Syria

    NARCIS (Netherlands)

    Bishaw, Z.

    2004-01-01

    Keywords: Wheat,Triticumspp., Barley,Hordeumvulgare L., Seed Systems, Formal Seed Sector, Informal Seed Sector, National Seed Program, Seed Source, Seed Selection, Seed Management, Seed Quality,

  5. MS Based Imaging of Barley Seed Development

    Institute of Scientific and Technical Information of China (English)

    Manuela Peukert; Andrea Matros; Hans-Peter Mock

    2012-01-01

    Spatially resolved analysis of metabolites and proteins is essential to model compartmentalized cellular processes in plants.Within recent years,tremendous progress has been made in MS based imaging (MSI) techniques,mostly MALDI MSI.The technology has been pioneered and is now widely applied in medicinal and pharmacological studies,and in recent years found its way into plant science (Kaspar et al.,2011; Peukert etal.,2012).We are interested in the elucidation of spatially resolved metabolic networks related to barley grain development.An understanding of developmentally and ecologically regulated processes affecting agronomical traits such as final grain weight,seed quality and stress tolerance is of outmost importance,as barley provides one of the staple foods.Barley also serves as a model plant for other cereals such as wheat.The presentation will introduce an untargeted MALDI MSI approach to the analysis of me-tabolite patterns during barley grain development.We analyzed longitudinal and cross sections from developing barley grains (3,7,10 and 14 days after pollination).In the presentation we will address spatial resolution,sensitivity and identification of unknown compounds will also be discussed.A major task is to connect the metabolite patterns to distinct cellular and physiological events.As an example,particular metabolite distributions indicative for nutrient transport into the developing endosperm will be shown.

  6. Search for endophytic diazotrophs in barley seeds

    Directory of Open Access Journals (Sweden)

    Myriam S. Zawoznik

    2014-06-01

    Full Text Available Eight endophytic isolates assigned to Pseudomonas, Azospirillum, and Bacillus genera according to pheno-genotypic features were retrieved from barley seeds under selective pressure for nitrogen-fixers. Genetic relationships among related isolates were investigated through RAPD. Six isolates displayed nitrogen-fixing ability, while all could biosynthesize indolacetic acid in vitro and showed no antibiosis effects against Azospirillum brasilense Az39, a recognized PGPR.

  7. Search for endophytic diazotrophs in barley seeds.

    Science.gov (United States)

    Zawoznik, Myriam S; Vázquez, Susana C; Díaz Herrera, Silvana M; Groppa, María D

    2014-01-01

    Eight endophytic isolates assigned to Pseudomonas, Azospirillum, and Bacillus genera according to pheno-genotypic features were retrieved from barley seeds under selective pressure for nitrogen-fixers. Genetic relationships among related isolates were investigated through RAPD. Six isolates displayed nitrogen-fixing ability, while all could biosynthesize indolacetic acid in vitro and showed no antibiosis effects against Azospirillum brasilense Az39, a recognized PGPR.

  8. Rhizopus oligosporus and yeast co-cultivation during barley tempeh fermentation--nutritional impact and real-time PCR quantification of fungal growth dynamics.

    Science.gov (United States)

    Feng, Xin Mei; Passoth, Volkmar; Eklund-Jonsson, Charlotte; Alminger, Marie Larsson; Schnürer, Johan

    2007-06-01

    Barley tempeh was produced by fermenting barley kernels with Rhizopus oligosporus. The potential of the yeasts Saccharomyces cerevisiae (three strains), S. boulardii (one strain), Pichia anomala (one strain) and Kluyveromyces lactis (one strain) to grow together with R. oligosporus during barley tempeh fermentation was evaluated. All yeast strains grew during the fermentation and even during cold storage of tempeh (P<0.01). The growth of yeasts slightly increased the ergosterol contents, but did not influence amino acid contents and compositions, and did not reduce phytate contents. Slight increases of vitamins B(6) and niacinamide, and slight decreases of B(1) and biotin were observed. Quantification of fungal growth is difficult during mixed species fermentations because ergosterol is found in all fungal species, and colony-forming-unit (cfu) estimations are not reliable for R. oligosporus and other sporulating fungi. Therefore, we developed a quantitative real-time PCR method for individually quantifying S. cerevisiae and R. oligosporus growth in barley tempeh. The PCR results were highly correlated with the ergosterol content of R. oligosporus and with the number of cfu of S. cerevisiae. Thus, real-time PCR is a rapid and selective method to quantify yeasts and R. oligosporus during mixed species fermentation of inhomogenous substrate such as barley tempeh.

  9. Aspects of the barley seed proteome during development and germination

    DEFF Research Database (Denmark)

    Finnie, Christine; Maeda, K.; Østergaard, O.;

    2004-01-01

    Analysis of the water-soluble barley seed proteome has led to the identification of proteins by MS in the major spots on two-dimensional gels covering the pi ranges 4-7 and 6-11. This provides the basis for in-depth studies of proteome changes during seed development and germination, tissue...

  10. Involvement of Alternative Splicing in Barley Seed Germination.

    Science.gov (United States)

    Zhang, Qisen; Zhang, Xiaoqi; Wang, Songbo; Tan, Cong; Zhou, Gaofeng; Li, Chengdao

    2016-01-01

    Seed germination activates many new biological processes including DNA, membrane and mitochondrial repairs and requires active protein synthesis and sufficient energy supply. Alternative splicing (AS) regulates many cellular processes including cell differentiation and environmental adaptations. However, limited information is available on the regulation of seed germination at post-transcriptional levels. We have conducted RNA-sequencing experiments to dissect AS events in barley seed germination. We identified between 552 and 669 common AS transcripts in germinating barley embryos from four barley varieties (Hordeum vulgare L. Bass, Baudin, Harrington and Stirling). Alternative 3' splicing (34%-45%), intron retention (32%-34%) and alternative 5' splicing (16%-21%) were three major AS events in germinating embryos. The AS transcripts were predominantly mapped onto ribosome, RNA transport machineries, spliceosome, plant hormone signal transduction, glycolysis, sugar and carbon metabolism pathways. Transcripts of these genes were also very abundant in the early stage of seed germination. Correlation analysis of gene expression showed that AS hormone responsive transcripts could also be co-expressed with genes responsible for protein biosynthesis and sugar metabolisms. Our RNA-sequencing data revealed that AS could play important roles in barley seed germination.

  11. Urethra-Sparing, Intraoperative, Real-Time Planned, Permanent-Seed Prostate Brachytherapy: Toxicity Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Zilli, Thomas [Department of Radiation Oncology, Centre hospitalier de l' Universite de Montreal-Hopital Notre-Dame, Montreal, QC (Canada); Taussky, Daniel, E-mail: daniel.taussky.chum@ssss.gouv.qc.ca [Department of Radiation Oncology, Centre hospitalier de l' Universite de Montreal-Hopital Notre-Dame, Montreal, QC (Canada); Donath, David; Le, Hoa Phong; Larouche, Renee-Xaviere; Beliveau-Nadeau, Dominique; Hervieux, Yannick; Delouya, Guila [Department of Radiation Oncology, Centre hospitalier de l' Universite de Montreal-Hopital Notre-Dame, Montreal, QC (Canada)

    2011-11-15

    Purpose: To report the toxicity outcome in patients with localized prostate cancer undergoing {sup 125}I permanent-seed brachytherapy (BT) according to a urethra-sparing, intraoperative (IO), real-time planned conformal technique. Methods and Materials: Data were analyzed on 250 patients treated consecutively for low- or intermediate-risk prostate cancer between 2005 and 2009. The planned goal was urethral V{sub 150} = 0. Acute and late genitourinary (GU), gastrointestinal (GI), and erectile toxicities were scored with the International Prostate Symptom Score (IPSS) questionnaire and Common Terminology Criteria for Adverse Events (version 3.0). Median follow-up time for patients with at least 2 years of follow-up (n = 130) was 34.4 months (range, 24-56.9 months). Results: Mean IO urethra V{sub 150} was 0.018% {+-} 0.08%. Mean prostate D{sub 90} and V{sub 100} on day-30 computed tomography scan were 158.0 {+-} 27.0 Gy and 92.1% {+-} 7.2%, respectively. Mean IPSS peak was 9.5 {+-} 6.3 1 month after BT (mean difference from baseline IPSS, 5.3). No acute GI toxicity was observed in 86.8% of patients. The 3-year probability of Grade {>=}2 late GU toxicity-free survival was 77.4% {+-} 4.0%, with Grade 3 late GU toxicity encountered in only 3 patients. Three-year Grade 1 late GI toxicity-free survival was 86.1% {+-} 3.2%. No patient presented Grade {>=}2 late GI toxicity. Of patients with normal sexual status at baseline, 20.7% manifested Grade {>=}2 erectile dysfunction after BT. On multivariate analysis, elevated baseline IPSS (p = 0.016) and high-activity sources (median 0.61 mCi) (p = 0.033) predicted increased Grade {>=}2 late GU toxicity. Conclusions: Urethra-sparing IO BT results in low acute and late GU toxicity compared with the literature. High seed activity and elevated IPSS at baseline increased long-term GU toxicity.

  12. Environmental and transgene expression effects on the barley seed proteome

    DEFF Research Database (Denmark)

    Finnie, Christine; Steenholdt, T.; Noguera, O.R.;

    2004-01-01

    The barley (Hordeum vulgare) cultivar Golden Promise is no longer widely used for malting, but is amenable to transformation and is therefore a valuable experimental cultivar. Its characteristics include high salt tolerance, however it is also susceptible to several fungal pathogens. Proteome....... Eleven of these were identified by mass spectrometric peptide mass mapping, including an abundant chitinase implicated in defence against fungal pathogens and a small heat-shock protein. To enable a comparison with transgenic seed protein patterns, differences in spot patterns between field...... with extra nitrogen. Finally, the fate of transgene products in barley seeds was followed. Spots containing two green fluorescent protein constructs and the herbicide resistance marker phosphinothricin acetyltransferase were observed in 2D-gel patterns of transgenic seeds and identified by mass spectrometry...

  13. Fungicides, seed dresser adjuvants and storage time in the control of Drechslera teres in barley seeds

    Directory of Open Access Journals (Sweden)

    Erlei Melo Reis

    2012-09-01

    Full Text Available In experiments conducted in laboratory, the effect of fungicides, seed dresser adjuvants and storage time in the control of Drechslera teres in seeds of barley cultivar BRS Elis, with 58% incidence, was quantified. Fungicides indicated by barley research (carboxin + thiram, difenoconazole and triadimenol compared with the mixture carbendazim + iprodione were tested. As seed dresser adjuvants, water (500m mL/100 Kg and a polymer (150 mL/100 Kg were used. Treated seeds were stored in paper bags and kept in the refrigerator at 5ºC. At 30-day intervals during six months, seeds were plated on semi-selective Reis's medium (1983. The best control was obtained by the mixtures carboxin + thiram and carbendazim + iprodione and the polymer as seed dresser. The control efficiency was improved by the storage time without negatively affecting seed germination. Due to the transmission efficiency, the fungus eradication in seeds should be pursued.

  14. Homeopathically prepared gibberellic acid and barley seed germination.

    Science.gov (United States)

    Hamman, B; Koning, G; Lok, K Him

    2003-07-01

    The potentisation process by which homeopathic preparations are produced raises the concern that these medicines have placebo effects only, since they theoretically no longer contain active molecules of the diluted substance. Plant models offer a method of examining the efficacy of homeopathically prepared solutions. This study examined the effects of homeopathically prepared gibberellic acid (HGA3) on the germination performance of barley (Hordeum vulgare L.) seeds. The effect of HGA3 (4-200 cH) on seed germination rate and seedling development was compared to that of the most commonly used form of gibberellic acid (GA3), 0.5 g l(-1), and control (distilled water). The extent and type of response was dependent on the vigour level of the seedlot. Treating seeds from three vigour groups in HGA3 consistently resulted in larger seedlings. High-vigour seeds treated with HGA3 4, 30 and 200 cH germinated faster, and roots of medium-vigour seedlots treated in HGA3 15 cH were longer. Biphasic effects of HGA3 were also demonstrated. As a plant model, germinating barley seeds successfully demonstrated the ability of HGA3 to produce a biological response.

  15. Real-time weed detection, decision making and patch spraying in maize, sugarbeet, winter wheat and winter barley

    DEFF Research Database (Denmark)

    Gerhards, R; Christensen, Svend

    2003-01-01

    online weed detection using digital image analysis, computer-based decision making and global positioning systems (GPS)-controlled patch spraying. In a 4-year study, herbicide use with this map-based approach was reduced in winter cereals by 60% for herbicides against broad-leaved weeds and 90% for grass......Information on temporal and spatial variation in weed seedling populations within agricultural fields is very important for weed population assessment and management. Most of all, it allows a potential reduction in herbicide use, when post-emergence herbicides are only applied to field sections...... with weed infestation levels higher than the economic weed threshold; a review of such work is provided. This paper presents a system for site-specific weed control in sugarbeet (Beta vulgaris L.), maize (Zea mays L.), winter wheat (Triticum aestivum L.) and winter barley (Hordeum vulgare L.), including...

  16. Plant seed species identification from chemical fingerprints: a high-throughput application of direct analysis in real time mass spectrometry.

    Science.gov (United States)

    Lesiak, Ashton D; Cody, Robert B; Dane, A John; Musah, Rabi A

    2015-09-01

    Plant species identification based on the morphological features of plant parts is a well-established science in botany. However, species identification from seeds has largely been unexplored, despite the fact that the seeds contain all of the genetic information that distinguishes one plant from another. Using seeds of genus Datura plants, we show here that the mass spectrum-derived chemical fingerprints for seeds of the same species are similar. On the other hand, seeds from different species within the same genus display distinct chemical signatures, even though they may contain similar characteristic biomarkers. The intraspecies chemical signature similarities on the one hand, and interspecies fingerprint differences on the other, can be processed by multivariate statistical analysis methods to enable rapid species-level identification and differentiation. The chemical fingerprints can be acquired rapidly and in a high-throughput manner by direct analysis in real time mass spectrometry (DART-MS) analysis of the seeds in their native form, without use of a solvent extract. Importantly, knowledge of the identity of the detected molecules is not required for species level identification. However, confirmation of the presence within the seeds of various characteristic tropane and other alkaloids, including atropine, scopolamine, scopoline, tropine, tropinone, and tyramine, was accomplished by comparison of the in-source collision-induced dissociation (CID) fragmentation patterns of authentic standards, to the fragmentation patterns observed in the seeds when analyzed under similar in-source CID conditions. The advantages, applications, and implications of the chemometric processing of DART-MS derived seed chemical signatures for species level identification and differentiation are discussed.

  17. Selective detection of viable seed-borne Acidovorax citrulli by real-time PCR with propidium monoazide

    Science.gov (United States)

    Tian, Qian; Feng, Jian-jun; Hu, Jie; Zhao, Wen-jun

    2016-01-01

    In recent years, use of the DNA-intercalating dye propidium monoazide (PMA) in real-time PCR has been reported as a novel method to detect viable bacteria in different types of samples, such as food, environmental, and microbiological samples. In this study, viable cells of Acidovorax citrulli, the causal agent of bacterial seedling blight and fruit blotch, were selectively detected and differentiated from dead cells by real-time fluorescent polymerase chain reaction amplification after the bacterial solution was treated with the DNA-binding dye PMA. The primers and TaqMan probe were based on the A. citrulli genome (Aave_1909, Gene ID: 4669443) and were highly specific for A. citrulli. The detection threshold of this assay was 103 colony-forming units per mL (CFU/mL) in pure cell suspensions containing viable and dead cells and infected watermelon seeds. Application of this assay enables the selective detection of viable cells of A. citrulli and facilitates monitoring of the pathogen in watermelon and melon seeds. PMID:27739469

  18. Radioprotective effects of ascorbic acid in barley seeds

    Energy Technology Data Exchange (ETDEWEB)

    Conger, B.V.

    1975-01-01

    Experiments were conducted to test the radioprotective effects of a naturally occurring reducing agent, ascorbic acid, on seeds (caryopses) of barley, Hordeum vulgare L. emend Lam. Seeds were soaked either before or after ..gamma.. or fission neutron irradiation in distilled water or ascorbic acid solutions ranging in concentration from 0.01 to 1.00 M. Results are reported as percentage germination, seedling height, seedling growth reduction, and (in one experiment) percent of cells with chromosome aberrations. As evidenced by both reduced germination and seedling growth, ascorbic acid was toxic when seeds were soaked for 1 hr at ambient temperature prior to irradiation and then planted immediately. When seeds were soaked in ascorbic acid before irradiation and soaked after irradiation in air-bubbled water at 0/sup 0/C for 18 hr, the toxicity disappeared, and a protective effect (which increased with increasing ascorbic acid concentration) was observed for ..gamma.. and, to a lesser extent, for neutron irradiation. Additional studies suggested that the protective effect was related to reduced hydration of the embryos of seeds soaked in ascorbic acid. Also, no radioprotective effect was observed when seeds were presoaked for 2 or 16 hr in 0.01 M ascorbic acid solutions buffered at pH 3 or pH 7. A protective effect was observed for seeds of 1.5 percent water content soaked after irradiation in an oxygen-bubbled ascorbic acid solution of 0.5 M but was not observed for seeds soaked in nitrogen-bubbled ascorbic acid. The protective effect against oxygen-dependent damage may be a result of interaction of ascorbic acid with radiation-induced free radicals.

  19. Proteome analysis of grain filling and seed maturation in barley.

    Science.gov (United States)

    Finnie, Christine; Melchior, Sabrina; Roepstorff, Peter; Svensson, Birte

    2002-07-01

    In monocotyledonous plants, the process of seed development involves the deposition of reserves in the starchy endosperm and development of the embryo and aleurone layer. The final stages of seed development are accompanied by an increase in desiccation tolerance and drying out of the mature seed. We have used two-dimensional gel electrophoresis for a time-resolved study of the changes in proteins that occur during seed development in barley (Hordeum vulgare). About 1,000 low-salt extractable protein spots could be resolved on the two-dimensional gels. Protein spots were divided into six categories according to the timing of appearance or disappearance during the 5-week period of comparison. Nineteen different proteins or protein fragments in 36 selected spots were identified by matrix-assisted laser-desorption ionization time of flight mass spectrometry (MS) or nano-electrospray tandem MS/MS. Some proteins were present throughout development (for example, cytosolic malate dehydrogenase), whereas others were associated with the early grain filling (ascorbate peroxidase) or desiccation (Cor14b) stages. Most noticeably, the development process is characterized by an accumulation of low-M(r) alpha-amylase/trypsin inhibitors, serine protease inhibitors, and enzymes involved in protection against oxidative stress. We present examples of proteins not previously experimentally observed, differential extractability of thiol-bound proteins, and possible allele-specific spot variation. Our results both confirm and expand on knowledge gained from previous analyses of individual proteins involved in grain filling and maturation.

  20. Effect of pulsed electric field on the germination of barley seeds

    DEFF Research Database (Denmark)

    Dymek, Katarzyna; Dejmek, Petr; Panarese, Valentina;

    2012-01-01

    This study explores metabolic responses of germinating barley seeds upon the application of pulsed electric fields (PEF). Malting barley seeds were steeped in aerated water for 24 h and PEF-treated at varying voltages (0 (control), 110, 160, 240, 320, 400 and 480 V). The seeds were then allowed...... to finish germination in saturated air. It is shown that exposure of germinating barley to PEF affects radicle emergence without significantly affecting the seeds’ gross metabolic activity, as quantified by isothermal calorimetry. An exploration of protein 2-DE profiles of both the embryo and the starchy...

  1. Viability of barley seeds after long-term exposure to outer side of international space station

    Science.gov (United States)

    Sugimoto, Manabu; Ishii, Makoto; Mori, Izumi C.; Elena, Shagimardanova; Gusev, Oleg A.; Kihara, Makoto; Hoki, Takehiro; Sychev, Vladimir N.; Levinskikh, Margarita A.; Novikova, Natalia D.; Grigoriev, Anatoly I.

    2011-09-01

    Barley seeds were exposed to outer space for 13 months in a vented metal container without a climate control system to assess the risk of physiological and genetic mutation during long-term storage in space. The space-stored seeds (S0 generation), with an 82% germination rate in 50 seeds, lost about 20% of their weight after the exposure. The germinated seeds showed normal growth, heading, and ripening. The harvested seeds (S1 generation) also germinated and reproduced (S2 generation) as did the ground-stored seeds. The culm length, ear length, number of seed, grain weight, and fertility of the plants from the space-stored seeds were not significantly different from those of the ground-stored seeds in each of the S0 and S1 generation. Furthermore, the S1 and S2 space-stored seeds respectively showed similar β-glucan content to those of the ground-stored seeds. Amplified fragment length polymorphism analysis with 16 primer combinations showed no specific fragment that appears or disappears significantly in the DNA isolated from the barley grown from the space-stored seeds. Though these data are derived from nine S0 space-stored seeds in a single exposure experiment, the results demonstrate the preservation of barley seeds in outer space for 13 months without phenotypic or genotypic changes and with healthy and vigorous growth in space.

  2. Genes controlling seed dormancy and pre-harvest sprouting in a rice-wheat-barley comparison

    DEFF Research Database (Denmark)

    Li, Chengdao; Ni, Peixiang; Francki, Michael;

    2004-01-01

    Pre-harvest sprouting results in significant economic loss for the grain industry around the world. Lack of adequate seed dormancy is the major reason for pre-harvest sprouting in the field under wet weather conditions. Although this trait is governed by multiple genes it is also highly heritable....... A major QTL controlling both pre-harvest sprouting and seed dormancy has been identified on the long arm of barley chromosome 5H, and it explains over 70% of the phenotypic variation. Comparative genomics approaches among barley, wheat and rice were used to identify candidate gene(s) controlling seed...... dormancy and hence one aspect of pre-harvest sprouting. The barley seed dormancy/pre-harvest sprouting QTL was located in a region that showed good synteny with the terminal end of the long arm of rice chromosome 3. The rice DNA sequences were annotated and a gene encoding GA20-oxidase was identified...

  3. Sensitive detection of Fusarium circinatum in pine seed by combining an enrichment procedure with a real-time polymerase chain reaction using dual-labeled probe chemistry.

    Science.gov (United States)

    Ioos, Renaud; Fourrier, Céline; Iancu, Gabriela; Gordon, Thomas R

    2009-05-01

    Fusarium circinatum is the causal agent of pitch canker disease on numerous Pinus spp. This aggressive fungus may infect pine seed cryptically and, therefore, can easily be spread long distances by the seed trade. F. circinatum has recently been listed as a quarantine organism in numerous countries throughout the world, which prompted the development of a specific and sensitive tool for the detection of this pathogen in conifer seed. A new detection protocol for F. circinatum based on a biological enrichment step followed by a real-time polymerase chain reaction (PCR) assay was developed. Several enrichment protocols were compared and a 72-h incubation of the seed with potato dextrose broth was the most efficient technique to increase F. circinatum biomass before DNA extraction. The relative accuracy, specificity, and sensitivity of the real-time PCR assay was evaluated in comparison with a previously published conventional PCR test on 420 seed DNA extracts. The real-time PCR described here proved to be highly specific and significantly more sensitive than the conventional PCR, and enabled the detection of F. circinatum in samples artificially contaminated with less than 1/1,000 infected seed, as well as in naturally infected samples. Last, in order to routinely check the quality of the seed DNA extracts, a primer-probe combination that targets a highly conserved region within the 18S ribosomal DNA in plants or fungi was successfully developed. This assay allows for quick and reliable detection of F. circinatum in seed, which can help to prevent long-distance spread of the pathogen via contaminated seed lots.

  4. WE-A-17A-09: Exploiting Electromagnetic Technologies for Real-Time Seed Drop Position Validation in Permanent Implant Brachytherapy

    Energy Technology Data Exchange (ETDEWEB)

    Racine, E [Departement de Radio-Oncologie et Centre de Recherche du CHU de Quebec, Quebec, QC (Canada); Hautvast, G [Biomedical Systems, Philips Group Innovation, Eindhoven, North Brabant (Netherlands); Binnekamp, D [Integrated Clinical Solutions and Marketing, Philips Healthcare, Best, DA (Netherlands); Beaulieu, L [Centre Hospitalier University de Quebec, Quebec, QC (Canada)

    2014-06-15

    Purpose: To report on preliminary results validating the performance of a specially designed LDR brachytherapy needle prototype possessing both electromagnetic (EM) tracking and seed drop detection abilities. Methods: An EM hollow needle prototype has been designed and constructed in collaboration with research partner Philips Healthcare. The needle possesses conventional 3D tracking capabilities, along with a novel seed drop detection mechanism exploiting local changes of electromagnetic properties generated by the passage of seeds in the needle's embedded sensor coils. These two capabilities are exploited by proprietary engineering and signal processing techniques to generate seed drop position estimates in real-time treatment delivery. The electromagnetic tracking system (EMTS) used for the experiment is the NDI Aurora Planar Field Generator. The experiment consisted of dropping a total of 35 seeds in a prismatic agarose phantom, and comparing the 3D seed drop positions of the EMTS to those obtained by an image analysis of subsequent micro-CT scans. Drop position error computations and statistical analysis were performed after a 3D registration of the two seed distributions. Results: Of the 35 seeds dropped in the phantom, 32 were properly detected by the needle prototype. Absolute drop position errors among the detected seeds ranged from 0.5 to 4.8 mm with mean and standard deviation values of 1.6 and 0.9 mm, respectively. Error measurements also include undesirable and uncontrollable effects such as seed motion upon deposition. The true accuracy performance of the needle prototype is therefore underestimated. Conclusion: This preliminary study demonstrates the potential benefits of EM technologies in detecting the passage of seeds in a hollow needle as a means of generating drop position estimates in real-time treatment delivery. Such tools could therefore represent a potentially interesting addition to existing brachytherapy protocols for rapid dosimetry

  5. Barley seed coating with free and immobilized alkaline phosphatase to improve P uptake and plant growth

    OpenAIRE

    Pilar Izquierdo, María Concepción; Ortega Santamaría, Natividad; Pérez Mateos, Manuel; Busto Núñez, Mª Dolores

    2012-01-01

    Coating barley seeds with free and immobilized alkaline phosphatase was investigated as a potential means to enhance plant utilization of accumulated soil phosphorus (P). Two coating techniques were studied: film-coating and pelleting. The highest phosphatase activity retention in the coating layer, ranging from 0·48 to 0·67, was observed when seeds were film-coated with phosphatase–polyresorcinol complex (PPC). The germination of seeds film-coated or pelleted with alkaline phosph...

  6. Characterization of the entire cystatin gene family in barley and their target cathepsin L-like cysteine-proteases, partners in the hordein mobilization during seed germination.

    Science.gov (United States)

    Martinez, Manuel; Cambra, Ines; Carrillo, Laura; Diaz-Mendoza, Mercedes; Diaz, Isabel

    2009-11-01

    Plant cystatins are inhibitors of cysteine-proteases of the papain C1A and legumain C13 families. Cystatin data from multiple plant species have suggested that these inhibitors act as defense proteins against pests and pathogens and as regulators of protein turnover. In this study, we characterize the entire cystatin gene family from barley (Hordeum vulgare), which contain 13 nonredundant genes, and identify and characterize their target enzymes, the barley cathepsin L-like proteases. Cystatins and proteases were expressed and purified from Escherichia coli cultures. Each cystatin was found to have different inhibitory capability against barley cysteine-proteases in in vitro inhibitory assays using specific substrates. Real-time reverse transcription-polymerase chain reaction revealed that inhibitors and enzymes present a wide variation in their messenger RNA expression patterns. Their transcripts were mainly detected in developing and germinating seeds, and some of them were also expressed in leaves and roots. Subcellular localization of cystatins and cathepsin L-like proteases fused to green fluorescent protein demonstrated the presence of both protein families throughout the endoplasmic reticulum and the Golgi complex. Proteases and cystatins not only colocalized but also interacted in vivo in the plant cell, as revealed by bimolecular fluorescence complementation. The functional relationship between cystatins and cathepsin L-like proteases was inferred from their common implication as counterparts of mobilization of storage proteins upon barley seed germination. The opposite pattern of transcription expression in gibberellin-treated aleurones presented by inhibitors and enzymes allowed proteases to specifically degrade B, C, and D hordeins stored in the endosperm of barley seeds.

  7. Real-time control of divertor detachment in H-mode with impurity seeding using Langmuir probe feedback in JET-ITER-like wall

    Science.gov (United States)

    Guillemaut, C.; Lennholm, M.; Harrison, J.; Carvalho, I.; Valcarcel, D.; Felton, R.; Griph, S.; Hogben, C.; Lucock, R.; Matthews, G. F.; Perez Von Thun, C.; Pitts, R. A.; Wiesen, S.; contributors, JET

    2017-04-01

    Burning plasmas with 500 MW of fusion power on ITER will rely on partially detached divertor operation to keep target heat loads at manageable levels. Such divertor regimes will be maintained by a real-time control system using the seeding of radiative impurities like nitrogen (N), neon or argon as actuator and one or more diagnostic signals as sensors. Recently, real-time control of divertor detachment has been successfully achieved in Type I ELMy H-mode JET-ITER-like wall discharges by using saturation current (I sat) measurements from divertor Langmuir probes as feedback signals to control the level of N seeding. The degree of divertor detachment is calculated in real-time by comparing the outer target peak I sat measurements to the peak I sat value at the roll-over in order to control the opening of the N injection valve. Real-time control of detachment has been achieved in both fixed and swept strike point experiments. The system has been progressively improved and can now automatically drive the divertor conditions from attached through high recycling and roll-over down to a user-defined level of detachment. Such a demonstration is a successful proof of principle in the context of future operation on ITER which will be extensively equipped with divertor target probes.

  8. Demarcation of mutant-carrying regions in barley plants after ethylmethane-sulfonate seed treatment

    DEFF Research Database (Denmark)

    Jacobsen, P.

    1966-01-01

    The branching pattern of the barley plant is analyzed and the anatomical structure of the resting barley embryo studied in longitudinal and cross-sections as well as by dissection techniques. The frequency and distribution of ethylmethane-sulfonate induced chloroplast and morphological seedling...... was obtained.The absence of cluster sharing allows the recognition in the barley plant of 8 mutually exclusive mutant sectors which never had a mutant cluster in common. The anatomical analysis proves that the barley embryo contains at least 6 separate shoot meristems or prospective shoot meristems, which...... will constitute mutually exclusive mutant sectors in the plant. The combined genetical and anatomical analysis reveals that in large seeds there are always 9 meristems leading to 9 mutually exclusive mutant sectors. Up to 7 additional meristems leading to mutually exclusive mutant sectors can be present...

  9. Feedback system for divertor impurity seeding based on real-time measurements of surface heat flux in the Alcator C-Mod tokamak.

    Science.gov (United States)

    Brunner, D; Burke, W; Kuang, A Q; LaBombard, B; Lipschultz, B; Wolfe, S

    2016-02-01

    Mitigation of the intense heat flux to the divertor is one of the outstanding problems in fusion energy. One technique that has shown promise is impurity seeding, i.e., the injection of low-Z gaseous impurities (typically N2 or Ne) to radiate and dissipate the power before it arrives to the divertor target plate. To this end, the Alcator C-Mod team has created a first-of-its-kind feedback system to control the injection of seed gas based on real-time surface heat flux measurements. Surface thermocouples provide real-time measurements of the surface temperature response to the plasma heat flux. The surface temperature measurements are inputted into an analog computer that "solves" the 1-D heat transport equation to deliver accurate, real-time signals of the surface heat flux. The surface heat flux signals are sent to the C-Mod digital plasma control system, which uses a proportional-integral-derivative (PID) algorithm to control the duty cycle demand to a pulse width modulated piezo valve, which in turn controls the injection of gas into the private flux region of the C-Mod divertor. This paper presents the design and implementation of this new feedback system as well as initial results using it to control divertor heat flux.

  10. Feedback system for divertor impurity seeding based on real-time measurements of surface heat flux in the Alcator C-Mod tokamak

    Science.gov (United States)

    Brunner, D.; Burke, W.; Kuang, A. Q.; LaBombard, B.; Lipschultz, B.; Wolfe, S.

    2016-02-01

    Mitigation of the intense heat flux to the divertor is one of the outstanding problems in fusion energy. One technique that has shown promise is impurity seeding, i.e., the injection of low-Z gaseous impurities (typically N2 or Ne) to radiate and dissipate the power before it arrives to the divertor target plate. To this end, the Alcator C-Mod team has created a first-of-its-kind feedback system to control the injection of seed gas based on real-time surface heat flux measurements. Surface thermocouples provide real-time measurements of the surface temperature response to the plasma heat flux. The surface temperature measurements are inputted into an analog computer that "solves" the 1-D heat transport equation to deliver accurate, real-time signals of the surface heat flux. The surface heat flux signals are sent to the C-Mod digital plasma control system, which uses a proportional-integral-derivative (PID) algorithm to control the duty cycle demand to a pulse width modulated piezo valve, which in turn controls the injection of gas into the private flux region of the C-Mod divertor. This paper presents the design and implementation of this new feedback system as well as initial results using it to control divertor heat flux.

  11. cDNA, amino acid carbohydrate sequence of barley seed-specific peroxidase BP 1

    DEFF Research Database (Denmark)

    Johansson, A.; Rasmussen, Søren Kjærsgård; Harthill, J.E.

    1992-01-01

    The major peroxidase of barley seed BP 1 was characterized. Previous studies showed a low carbohydrate content, low specific activity and tissue-specific expression, and suggested that this basic peroxidase could be particularly useful in the elucidation of the structure-function relationship...... plant modified-type structure, Man-alpha-1-6(Xyl-beta-1-2)Man-beta-1-4GlcNAc-beta-1-4(Fuc-alpha-1-3)GlcNAc. The BP 1 gene was RFLP-mapped on barley chromosome 3, and we propose Prx5 as the name for this new peroxidase locus....

  12. Molecular Clustering Interrelationships and Carbohydrate Conformation in Hull and Seeds Among Barley Cultivars

    Energy Technology Data Exchange (ETDEWEB)

    N Liu; P Yu

    2011-12-31

    The objective of this study was to use molecular spectral analyses with the diffuse reflectance Fourier transform infrared spectroscopy (DRIFT) bioanlytical technique to study carbohydrate conformation features, molecular clustering and interrelationships in hull and seed among six barley cultivars (AC Metcalfe, CDC Dolly, McLeod, CDC Helgason, CDC Trey, CDC Cowboy), which had different degradation kinetics in rumen. The molecular structure spectral analyses in both hull and seed involved the fingerprint regions of ca. 1536-1484 cm{sup -1} (attributed mainly to aromatic lignin semicircle ring stretch), ca. 1293-1212 cm{sup -1} (attributed mainly to cellulosic compounds in the hull), ca. 1269-1217 cm{sup -1} (attributed mainly to cellulosic compound in the seeds), and ca. 1180-800 cm{sup -1} (attributed mainly to total CHO C-O stretching vibrations) together with an agglomerative hierarchical cluster (AHCA) and principal component spectral analyses (PCA). The results showed that the DRIFT technique plus AHCA and PCA molecular analyses were able to reveal carbohydrate conformation features and identify carbohydrate molecular structure differences in both hull and seeds among the barley varieties. The carbohydrate molecular spectral analyses at the region of ca. 1185-800 cm{sup -1} together with the AHCA and PCA were able to show that the barley seed inherent structures exhibited distinguishable differences among the barley varieties. CDC Helgason had differences from AC Metcalfe, MeLeod, CDC Cowboy and CDC Dolly in carbohydrate conformation in the seed. Clear molecular cluster classes could be distinguished and identified in AHCA analysis and the separate ellipses could be grouped in PCA analysis. But CDC Helgason had no distinguished differences from CDC Trey in carbohydrate conformation. These carbohydrate conformation/structure difference could partially explain why the varieties were different in digestive behaviors in animals. The molecular spectroscopy

  13. Real-time systems

    OpenAIRE

    Badr, Salah M.; Bruztman, Donald P.; Nelson, Michael L.; Byrnes, Ronald Benton

    1992-01-01

    This paper presents an introduction to the basic issues involved in real-time systems. Both real-time operating sys and real-time programming languages are explored. Concurrent programming and process synchronization and communication are also discussed. The real-time requirements of the Naval Postgraduate School Autonomous Under Vehicle (AUV) are then examined. Autonomous underwater vehicle (AUV), hard real-time system, real-time operating system, real-time programming language, real-time sy...

  14. Biochemical and Molecular Characterization of a Barley Seed ß-Glucosidase

    DEFF Research Database (Denmark)

    Leah, R.; Kigel, J.; Svendsen, I.

    1995-01-01

    A 60-kDa ß-glucosidase (BGQ60) was purified and characterized from seeds of barley (Hordeum vulgare L.). BGQ60 catalytic activity was restricted to the cleavage of short-chain oligosaccharides composed of(1, 2) -,(1, 2, 3) -, and/or(1, 2, 3, 4) -ß-linked glucose or mannose units. These oligosacch......A 60-kDa ß-glucosidase (BGQ60) was purified and characterized from seeds of barley (Hordeum vulgare L.). BGQ60 catalytic activity was restricted to the cleavage of short-chain oligosaccharides composed of(1, 2) -,(1, 2, 3) -, and/or(1, 2, 3, 4) -ß-linked glucose or mannose units....... These oligosaccharides are the primary products of endosperm cell wall polysaccharide hydrolysis by other enzymes. In keeping with this, complete hydrolysis of the major polysaccharide of barley starchy endosperm cell wall, (1-3,1-4)-ß-glucan, to free glucose was shown to require the concerted action of endo-(1-3,1-4)-ß...... interrupted by 9 introns. The complete nucleotide sequence of the bgq60 structural gene represents the first characterized plant gene encoding a ß-glucosidase. The barley BGQ60 is a novel plant ß-glucosidase with a hitherto undescribed specific enzymatic activity. The possible biological functions of BGQ60...

  15. Evidence for osmotic regulation of hydrolytic enzyme production in germinating barley seeds.

    Science.gov (United States)

    Jones, R L; Armstrong, J E

    1971-08-01

    alpha-Amylase levels in intact seeds of barley (Hordeum vulgare L. cv. Himalaya) reach a maximum at 3 to 4 days of germination while gibberellin levels continue to increase beyond 6 days of germination. In contrast to its effect on half seeds, gibberellic acid does not increase the total amount of alpha-amylase produced in germinating seeds. The inability of gibberellic acid to stimulate alpha-amylase production is not related to its availability; rather, evidence suggests that a factor(s) in whole seeds prevents further enhancement of alpha-amylase formation and accumulation. Hydrolysis products accumulate in the subaleurone space of the endosperm of germinating seeds up to concentrations of 570 milliosmolar. Chromatography of these hydrolysis products indicate the presence of maltose and glucose. Calculations based on reducing sugar determinations show that glucose accounts for as much as 57% of the solutes present in the endosperm fluid. Both maltose and glucose in the range of 0.2 to 0.4 M effectively inhibit the production of alpha-amylase by isolated barley aleurone layers. This inhibition is quantitatively similar to that brought about by solutions of polyethylene glycol and mannitol. On the basis of these data we propose that hydrolysis products which accumulate in the starchy endosperm of germinating seeds function to regulate the production of hydrolytic enzymes by the aleurone layer.

  16. Barley (Hordeum vulgare L.) low phytic acid 1-1: an endosperm-specific, filial determinant of seed total phosphorus.

    Science.gov (United States)

    Raboy, Victor; Cichy, Karen; Peterson, Kevin; Reichman, Sarah; Sompong, Utumporn; Srinives, Peerasak; Saneoka, Hirofumi

    2014-01-01

    Inositol hexaphosphate (Ins P6 or "phytic acid") typically accounts for 75 (± 10%) of seed total phosphorus (P). In some cases, genetic blocks in seed Ins P6 accumulation can also alter the distribution or total amount of seed P. In nonmutant barley (Hordeum vulgare L.) caryopses, ~80% of Ins P6 and total P accumulate in the aleurone layer, the outer layer of the endosperm, with the remainder in the germ. In barley low phytic acid 1-1 (Hvlpa1-1) seed, both endosperm Ins P6 and total P are reduced (~45% and ~25%, respectively), but germs are phenotypically wild type. This translates into a net reduction in whole-seed total P of ~15%. Nutrient culture studies demonstrate that the reduction in endosperm total P is not due to a reduction in the uptake of P into the maternal plant. Genetic tests (analyses of testcross and F2 seed) reveal that the Hvlpa1-1 genotype of the filial seed conditions the seed total P reduction; sibling seed in the same head of barley that differ in their Hvlpa1-1 genotype (heterozygous vs. homozygous recessive) differ in their total P (normal vs. reduced, respectively). Therefore, Hvlpa1 functions as a seed-specific or filial determinant of barley endosperm total P.

  17. Barley Seed Germination/Root Elongation Toxicity Test For Evaluation Of Sludge Pre-Treatment

    DEFF Research Database (Denmark)

    Eriksson, Eva; Kusk, Kresten Ole; Barrett Sørensen, Mie

    Application of sludge from wastewater treatment plants (WWTPs) on agricultural land is an approach for nutrient recycling that rise challenges due to recalcitrant and harmful pollutants. In this study we assessed the feasibility of a seed germination test to evaluate sludge ecotoxicity and compared...... germination responses from two test parameters, root elongation and seed germination (sprouts elongation) of the barley (Hordeum vulgare). 2nd objective was to evaluate sewage sludge pre-treatments at batch-scale of sludge samples from two WWTPs using anaerobic digestion, and thermal and ozonation pre...

  18. A Role for Reactive Oxygen Species Produced by NADPH Oxidases in the Embryo and Aleurone Cells in Barley Seed Germination

    OpenAIRE

    Yushi Ishibashi; Shinsuke Kasa; Masatsugu Sakamoto; Nozomi Aoki; Kyohei Kai; Takashi Yuasa; Atsushi Hanada; Shinjiro Yamaguchi; Mari Iwaya-Inoue

    2015-01-01

    Reactive oxygen species (ROS) promote the germination of several seeds, and antioxidants suppress it. However, questions remain regarding the role and production mechanism of ROS in seed germination. Here, we focused on NADPH oxidases, which produce ROS. After imbibition, NADPH oxidase mRNAs were expressed in the embryo and in aleurone cells of barley seed; these expression sites were consistent with the sites of ROS production in the seed after imbibition. To clarify the role of NADPH oxidas...

  19. Validation of a newly developed hexaplex real-time PCR assay for screening for presence of GMOs in food, feed and seed.

    Science.gov (United States)

    Bahrdt, C; Krech, A B; Wurz, A; Wulff, D

    2010-03-01

    For years, an increasing number and diversity of genetically modified plants has been grown on a commercial scale. The need for detection and identification of these genetically modified organisms (GMOs) calls for broad and at the same time flexible high throughput testing methods. Here we describe the development and validation of a hexaplex real-time polymerase chain reaction (PCR) screening assay covering more than 100 approved GMOs containing at least one of the GMO targets of the assay. The assay comprises detection systems for Cauliflower Mosaic Virus 35S promoter, Agrobacterium tumefaciens NOS terminator, Figwort Mosaic Virus 34S promoter and two construct-specific sequences present in novel genetically modified soybean and maize that lack common screening elements. Additionally a detection system for an internal positive control (IPC) indicating the presence or absence of PCR inhibiting substances was included. The six real-time PCR systems were allocated to five detection channels showing no significant crosstalk between the detection channels. As part of an extensive validation, a limit of detection (LOD(abs)) GMO detection system was still shown in highly asymmetric target situations in the presence of 1,000 copies of all other GMO targets of each detection channel. Furthermore, the applicability to a broad sample spectrum and reliable indication of inhibition by the IPC system was demonstrated. The presented hexaplex assay offers sensitive and reliable detection of GMOs in processed and unprocessed food, feed and seed samples with high efficiency.

  20. Improvement of seedling establishment under flood condition by seed coating with molybdenum compounds for wheat and barley

    Directory of Open Access Journals (Sweden)

    Yoshitaka Hara

    2016-04-01

    Full Text Available Wheat and barley are often cultivated also in paddy fields in winter in Japan. The drainage of paddy fields is often poor. The seedling establishment of wheat and barley is more prone to become poor if it rains heavily after sowing. The flooding damage on seedling establishment is thought to be caused by many factors. The generation of sulfide ions in flooded and reduced soil is thought to be one factor of the flooding damage. In this study, the effect of seed coating with molybdenum compounds, which suppress the generation of sulfide ions, on the flooding damage of wheat and barley seedling establishment. Two poorly soluble molybdenum compounds were coated on wheat or barley seeds at different amounts. Coated seeds were sown in soil and soon flooded for 2 d at 20 °C. When seeds were not coated with molybdenum compounds, rates of seedling establishment were no more than 32%. However, when any molybdenum compounds of .05–.5 mol-Mo kg−1 were coated, seedling establishment was significantly improved and rates of seedling establishment were no less than 54%. However, when sown seed were not flooded, the establishment rates of the seeds, which were coated with a molybdenum compound of no less than .1 or .2 mol-Mo kg-1, were significantly decreased. Accordingly, coating of molybdenum compounds could improve the seedling establishment of coated seeds under flooded condition, but might impair the seedling establishment of coated seeds under unflooded condition.

  1. A Role for Reactive Oxygen Species Produced by NADPH Oxidases in the Embryo and Aleurone Cells in Barley Seed Germination.

    Directory of Open Access Journals (Sweden)

    Yushi Ishibashi

    Full Text Available Reactive oxygen species (ROS promote the germination of several seeds, and antioxidants suppress it. However, questions remain regarding the role and production mechanism of ROS in seed germination. Here, we focused on NADPH oxidases, which produce ROS. After imbibition, NADPH oxidase mRNAs were expressed in the embryo and in aleurone cells of barley seed; these expression sites were consistent with the sites of ROS production in the seed after imbibition. To clarify the role of NADPH oxidases in barley seed germination, we examined gibberellic acid (GA / abscisic acid (ABA metabolism and signaling in barley seeds treated with diphenylene iodonium chloride (DPI, an NADPH oxidase inhibitor. DPI significantly suppressed germination, and suppressed GA biosynthesis and ABA catabolism in embryos. GA, but not ABA, induced NADPH oxidase activity in aleurone cells. Additionally, DPI suppressed the early induction of α-amylase by GA in aleurone cells. These results suggest that ROS produced by NADPH oxidases promote GA biosynthesis in embryos, that GA induces and activates NADPH oxidases in aleurone cells, and that ROS produced by NADPH oxidases induce α-amylase in aleurone cells. We conclude that the ROS generated by NADPH oxidases regulate barley seed germination through GA / ABA metabolism and signaling in embryo and aleurone cells.

  2. A Role for Reactive Oxygen Species Produced by NADPH Oxidases in the Embryo and Aleurone Cells in Barley Seed Germination.

    Science.gov (United States)

    Ishibashi, Yushi; Kasa, Shinsuke; Sakamoto, Masatsugu; Aoki, Nozomi; Kai, Kyohei; Yuasa, Takashi; Hanada, Atsushi; Yamaguchi, Shinjiro; Iwaya-Inoue, Mari

    2015-01-01

    Reactive oxygen species (ROS) promote the germination of several seeds, and antioxidants suppress it. However, questions remain regarding the role and production mechanism of ROS in seed germination. Here, we focused on NADPH oxidases, which produce ROS. After imbibition, NADPH oxidase mRNAs were expressed in the embryo and in aleurone cells of barley seed; these expression sites were consistent with the sites of ROS production in the seed after imbibition. To clarify the role of NADPH oxidases in barley seed germination, we examined gibberellic acid (GA) / abscisic acid (ABA) metabolism and signaling in barley seeds treated with diphenylene iodonium chloride (DPI), an NADPH oxidase inhibitor. DPI significantly suppressed germination, and suppressed GA biosynthesis and ABA catabolism in embryos. GA, but not ABA, induced NADPH oxidase activity in aleurone cells. Additionally, DPI suppressed the early induction of α-amylase by GA in aleurone cells. These results suggest that ROS produced by NADPH oxidases promote GA biosynthesis in embryos, that GA induces and activates NADPH oxidases in aleurone cells, and that ROS produced by NADPH oxidases induce α-amylase in aleurone cells. We conclude that the ROS generated by NADPH oxidases regulate barley seed germination through GA / ABA metabolism and signaling in embryo and aleurone cells.

  3. Comparison of Intraoperatively Built Custom Linked Seeds Versus Loose Seed Gun Applicator Technique Using Real-Time Intraoperative Planning for Permanent Prostate Brachytherapy

    Energy Technology Data Exchange (ETDEWEB)

    Zauls, A. Jason; Ashenafi, Michael S. [Department of Radiation Oncology, Hollings Cancer Center, Medical University of South Carolina, Charleston, SC (United States); Onicescu, Georgiana [Department of Biostatistics and Epidemiology, Hollings Cancer Center, Medical University of South Carolina, Charleston, SC (United States); Clarke, Harry S. [Department of Urology, Hollings Cancer Center, Medical University of South Carolina, Charleston, SC (United States); Marshall, David T., E-mail: marshadt@musc.edu [Department of Radiation Oncology, Hollings Cancer Center, Medical University of South Carolina, Charleston, SC (United States)

    2011-11-15

    Purpose: To report our dosimetric results using a novel push-button seed delivery system that constructs custom links of seeds intraoperatively. Methods and Materials: From 2005 to 2007, 43 patients underwent implantation using a gun applicator (GA), and from 2007 to 2008, 48 patientsunderwent implantation with a novel technique allowing creation of intraoperatively built custom links of seeds (IBCL). Specific endpoint analyses were prostate D90% (pD90%), rV100% > 1.3 cc, and overall time under anesthesia. Results: Final analyses included 91 patients, 43 GA and 48 IBCL. Absolute change in pD90% ({Delta}pD90%) between intraoperative and postoperative plans was evaluated. Using GA method, the {Delta}pD90% was -8.1Gy and -12.8Gy for I-125 and Pd-103 implants, respectively. Similarly, the IBCL technique resulted in a {Delta}pD90% of -8.7Gy and -9.8Gy for I-125 and Pd-103 implants, respectively. No statistically significant difference in {Delta}pD90% was found comparing methods. The GA method had two intraoperative and 10 postoperative rV100% >1.3 cc. For IBCL, five intraoperative and eight postoperative plans had rV100% >1.3 cc. For GA, the mean time under anesthesia was 75 min and 87 min for Pd-103 and I-125 implants, respectively. For IBCL, the mean time was 86 and 98 min for Pd-103 and I-125. There was a statistical difference between the methods when comparing mean time under anesthesia. Conclusions: Dosimetrically relevant endpoints were equivalent between the two methods. Currently, time under anesthesia is longer using the IBCL technique but has decreased over time. IBCL is a straightforward brachytherapy technique that can be implemented into clinical practice as an alternative to gun applicators.

  4. Extraction of high-quality RNA from germinating barley (Hordeum vulgare L.) seeds containing high levels of starch.

    Science.gov (United States)

    Comparative evaluation of gene expression levels can lead to improved understanding of the gene networks underlying traits of economic importance. Extraction of high-quality RNA from germinating barley seeds that contain high levels of starch is of vital importance for analysing the expression of ca...

  5. Proteome analysis of barley seeds: Identification of major proteins from two-dimensional gels (pl 4-7)

    DEFF Research Database (Denmark)

    Østergaard, O.; Finnie, Christine; Laugesen, S.;

    2004-01-01

    -soluble proteins in extracts of mature barley (Hordeum vulgare) seeds and to follow their fate during germination. About 1200 and 600 spots of p/ 4-7 were detected on 2-D gels by silver staining and colloidal Coomassie Brilliant Blue staining, respectively. About 300 spots were selected for in-gel digestion...

  6. From Proteomics to Structural Studies of Cytosolic/Mitochondrial-Type Thioredoxin Systems in Barley Seeds

    Institute of Scientific and Technical Information of China (English)

    Azar Shahpiri; Birte Svensson; Christine Finnie

    2009-01-01

    Thioredoxins (Trx) are ubiquitous proteins that participate in thiol disulfide reactions via two active site cysteine residues,allowing Trx to reduce disulfide bonds in target proteins.Recent progress in proteome analysis has resulted in identification of a wide range of potential target proteins for Trx,indicating that Trx plays a key role in several aspects of cell metabolism.In contrast to other organisms,plants contain multiple forms of Trx that are classified based on their primary structures and sub-cellular localization.The reduction of cytosolic and mitochondrial types of Trx is dependenl on NADPH and catalyzed by NADPH-dependent thioredoxin reductase (NTR).In barley,two isoforms each of Trx and NTR have been identified and investigated using proteomics,gene expression,and structural studies.This review outlines the diverse roles suggested for cytosolic/mitochondrial-type Trx systems in cereal seeds and summarizes the current knowl-edge of the barley system including recent data on function,regulation,interactions,and structure.Directions for future research are discussed.

  7. Antioxidant activity of 100% and 80% methanol extracts from barley seeds (Hordeum vulgare L.): stabilization of sunflower oil

    Energy Technology Data Exchange (ETDEWEB)

    Anwar, F.; Abdul Qayyum, H. M.; Hussein, A. I.; Iqbal, S.

    2010-07-01

    The antioxidant potential of 100% and 80% methanol extracts from the seeds of three barley varieties (Jou 83, Jou 87 and Haider 93) was assessed. The extract yields from barley seeds ranged from 3.23% (Haider 93,100% methanol) to 5.31% (Jou 83, 80% methanol). The total phenolic contents, DPPH radical scavenging activity (IC50 values) and inhibition of linoleic acid oxidation of barley seed extracts (BSE) were determined to be 88.1-145.7 mg/100g, 90.8-168.6 {mu}g/mL and 62.6-74.6%, respectively. The antioxidant effectiveness of BSE was also assessed by stabilizing sunflower oil (SFO) with BSE at a concentration of 600 ppm (oil weight basis). The stabilized (treated with extract) and the control (without extract addition) SFO samples were subjected to accelerated (oven heating at 60 degree centigrade for 30 days, 8 h heating cycle/day) storage. These were analyzed at regular intervals for the extent of oxidative changes according to the measurements of their contents of peroxide value, para-anisidine value, conjugated dienes and conjugated trienes. Generally, the 80% methanol extract of barely seeds demonstrated better antioxidant action than the 100% methanol extract. The antioxidant activity of BSE was also found to be considerably varied among the varieties tested. The present results suggest that antioxidant extracts from barely seeds might be used to protect vegetable oils from oxidation. (Author) 32 refs.

  8. Comparison of frequency distributions of doubled haploid and single seed descent lines in barley.

    Science.gov (United States)

    Choo, T M; Reinbergs, E; Park, S J

    1982-09-01

    Both doubled haploid (DH) and single seed descent (SSD) methods were used to derive homozygous lines from two crosses of barley. The frequency distributions of grain yield, heading date, and plant height of the DH and SSD lines were compared by the Mann-Whitney U test, Kolmogorov-Smirnov twosample test and Wald-Wolfowitz runs test. It was found that the DH lines distributed in the same manner as the SSD lines with respect to the three characters. The results indicated that although the SSD method had more opportunity for recombination than the DH method, it did not produce a sample of recombinants which differed significantly from the DH sample; thus both methods were equally efficient for use in deriving homozygous lines from F1 hybrids in a relatively short time.

  9. Proteome analysis of dissected barley seed tissue during germination and radicle elongation

    DEFF Research Database (Denmark)

    Bønsager, Birgit Christine

    2007-01-01

    Cereal grains are vital components of our diet, and therefore understanding of the biology of breakage of dormancy and initiation of germination very important. These processes are far from fully understood, despite extensive studies, and no specific markers for germination have been identified...... at the protein or the DNA level. In addition, germination of barley seeds is of interest for the brewing industry since this process corresponds to the steeping process that starts the industrial malting. In the present study a proteomics approach was employed to understand the initial changes in the water...... stress, storage breakdown, folding, and housekeeping were identified and tracked through 72 h PI. APX and the other enzymes involved in the ascorbate-glutathione cycle (dehydroascorbate reductase, monodehydroascorbate reductase, and glutathione reductase) were thought to have an important function during...

  10. Spatio-temporal profiling and degradation of α-amylase isozymes during barley seed germination

    DEFF Research Database (Denmark)

    Bak-Jensen, K.S.; Laugesen, S.; Østergaard, O.

    2007-01-01

    Ten genes from two multigene families encode barley alpha-amylases. To gain insight into the occurrence and fate of individual isoforms during seed germination, the alpha-amylase repertoire was mapped by using a proteomics approach consisting of 2D gel electrophoresis, western blotting, and mass...... identified only by immunostaining. Mass spectrometry identified 12 full-length forms and 12 fragments from the cultivar Barke. Products of both alpha-amylase 2 entries co-migrated in five full-length and one fragment spot. The alpha-amylase abundance and the number of fragments increased during germination...... and near the C-terminus. Only two shorter fragments were identified of the other alpha-amylase 2 (gi|166985). The 2D gels of dissected tissues showed alpha-amylase degradation to be confined to endosperm. In contrast, the aleurone layer contained essentially only full-length alpha-amylase forms. While only...

  11. Spatio-temporal profiling and degradation of alpha-amylase isozymes during barley seed germination

    DEFF Research Database (Denmark)

    Bak-Jensen, K.S.; Laugesen, Sabrina; Østergaard, Ole

    2007-01-01

    Ten genes from two multigene families encode barley alpha-amylases. To gain insight into the occurrence and fate of individual isoforms during seed germination, the alpha-amylase repertoire was mapped by using a proteomics approach consisting of 2D gel electrophoresis, western blotting, and mass...... increased during germination. Assessing the fragment minimum chain length by peptide mass fingerprinting suggested that alpha-amylase 2 ( gi vertical bar 4699831) initially was cleaved just prior to domain B that protrudes from the (beta alpha)(8)-barrel between beta-strand 3 and alpha-helix 3, followed...... by cleavage on the C-terminal side of domain B and near the C-terminus. Only two shorter fragments were identified of the other alpha-amylase 2 (gi vertical bar 166985). The 2D gels of dissected tissues showed alpha-amylase degradation to be confined to endosperm. In contrast, the aleurone layer contained...

  12. Real Time Systems

    DEFF Research Database (Denmark)

    Christensen, Knud Smed

    2000-01-01

    Describes fundamentals of parallel programming and a kernel for that. Describes methods for modelling and checking parallel problems. Real time problems.......Describes fundamentals of parallel programming and a kernel for that. Describes methods for modelling and checking parallel problems. Real time problems....

  13. THE STUDY OF THE INFLUENCE OF AN ALTERNATING ELECTROMAGNETIC FIELD OF COMMERCIAL FREQUENCY ON CROP SEED QUALITY OF SPRING BARLEY

    Directory of Open Access Journals (Sweden)

    Zholobova M. V.

    2015-04-01

    Full Text Available The process of the presowing seed treatment with alternating electromagnet field of the commercial frequency (EMF CF 50 Hz is economically efficient. The use of the EMF CF 50 Hz in the unit with ring pole pieces allows increasing the germinating energy and power. The electromagnet seed treatment in the units with ring pole pieces is the perspective process of the presowing treatment which does not make unhealthy influence on the operating personnel. At the presowing treatment we have to take into account three factors, they are the arrangement of seeds in the working chamber, the time of treatment and the seed humidity. In this article the authors recommend to promote the unit consisting of a magnetic circuit with ring pole pieces of the rectangular cross-section, a magnetizing coil and an embedding of the non-magnetic material in the working chamber which allows increasing the germinating energy of spring barley by 10% and the germination by 7%. As field experiences have shown the highest seed germination and good crop capacity take place in the work chamber zone with boundaries R1=0,012 m and R2=0,035 m at seed humidity from 12% till 18% and the time of seed treatment is 0,9-2,75 s. The addition to crop capacity has run to 7% as a result of the spring barley treatment

  14. Real-time shadows

    CERN Document Server

    Eisemann, Elmar; Assarsson, Ulf; Wimmer, Michael

    2011-01-01

    Important elements of games, movies, and other computer-generated content, shadows are crucial for enhancing realism and providing important visual cues. In recent years, there have been notable improvements in visual quality and speed, making high-quality realistic real-time shadows a reachable goal. Real-Time Shadows is a comprehensive guide to the theory and practice of real-time shadow techniques. It covers a large variety of different effects, including hard, soft, volumetric, and semi-transparent shadows.The book explains the basics as well as many advanced aspects related to the domain

  15. Carbon nanofibers suppress fungal inhibition of seed germination of maize (Zea mays) and barley (Hordeum vulgare L.) crop

    Science.gov (United States)

    Joshi, Anjali; Sharma, Arti; Nayyar, Harsh; Verma, Gaurav; Dharamvir, Keya

    2015-08-01

    Carbon nanofibers (CNFs) are one of allotropes of carbon, consists of graphene layers arrangement in the form of stacked cones or like a cup diameter in nanometer and several millimeters in length. Their extraordinary mechanical, chemical and electronic properties are due to their small size. CNFs have been successfully applied in field of medicine in variety of diagnostic methods. They proven to be an excellent system for drug delivery, tissue regeneration, biosensor etc. This research focuses the applications of CNFs in all fields of Agriculture. In the we treated some fungal disease seed of maize and barley using functionalised CNFs. We find that the tested seeds grow just as well as the healthy seeds whereas the untreated fungal disease seeds, by themselves show very poor germination and seedling growth. This simple experiment shows the extraordinary ability of Carbon nanofibers in carrying effectively inside the germinated seeds.

  16. Carbon nanofibers suppress fungal inhibition of seed germination of maize (Zea mays) and barley (Hordeum vulgare L.) crop

    Energy Technology Data Exchange (ETDEWEB)

    Joshi, Anjali, E-mail: joshianjali1982@gmail.com; Sharma, Arti [Centre For Nanoscience and Nanotechnology, Panjab University, Chandigarh (India); Nayyar, Harsh [Department of Botany, Panjab University, Chandigarh (India); Verma, Gaurav [Dr. SS Bhatnagar University Institute of Chemical Engineering and Technology, Panjab University, Chandigarh (India); Dharamvir, Keya [Department of Physics, Panjab University, Chandigarh (India)

    2015-08-28

    Carbon nanofibers (CNFs) are one of allotropes of carbon, consists of graphene layers arrangement in the form of stacked cones or like a cup diameter in nanometer and several millimeters in length. Their extraordinary mechanical, chemical and electronic properties are due to their small size. CNFs have been successfully applied in field of medicine in variety of diagnostic methods. They proven to be an excellent system for drug delivery, tissue regeneration, biosensor etc. This research focuses the applications of CNFs in all fields of Agriculture. In the we treated some fungal disease seed of maize and barley using functionalised CNFs. We find that the tested seeds grow just as well as the healthy seeds whereas the untreated fungal disease seeds, by themselves show very poor germination and seedling growth. This simple experiment shows the extraordinary ability of Carbon nanofibers in carrying effectively inside the germinated seeds.

  17. On the modulation effect of pulsing and static magnetic fields and mechanical vibrations on barley seed hydration.

    Science.gov (United States)

    Amyan, Armine; Ayrapetyan, Sinerik

    2004-01-01

    The changes of wet and dry weights of barley seed in different periods of swelling were studied in seeds treated with Extremely Low Frequency Electromagnetic Fields (ELF EMF), Static Magnetic Fields (SMF) and Mechanical Vibrations (MV) in cold (4 degrees C) and warm (20 degrees C) distilled water as well as in seeds non-treated (control). The metabolic dependent seed hydration, dry weight loss and water binding in seed were modulated by preliminary EMF, SMF and MV-induced treatment of distilled water. The specific electrical conductivity (SEC) of control and treated distilled water was measured before the seed incubation. Frequency and intensity "windows" (i.e. range of frequency or intensity) for the effect of EMF, MV and SMF (correspondingly) on seed hydration, solubility and water binding in seed were studied. These "windows" were different in various phases of seed swelling. It is suggested that water structure modification is the result of valence angle changes (SMF and EMF) and dipole molecules vibration (EMF and MV) has different effects on the process of hydration, solubility and water binding in seed. These results are important from the point of understanding the mechanisms of the biological effect of EMF, as well as from the point of agriculture.

  18. Colonization pattern of the biocontrol strain Pseudomonas chlororaphis MA 342 on barley seeds visualized by using green fluorescent protein.

    Science.gov (United States)

    Tombolini, R; van der Gaag, D J; Gerhardson, B; Jansson, J K

    1999-08-01

    Pseudomonas chlororaphis MA 342 is a potent biocontrol agent that can be used against several seed-borne diseases of cereal crops, including net blotch of barley caused by the fungus Drechslera teres. In this study, strain MA 342 was tagged with the gfp gene (encoding the green fluorescent protein) in order to study the fate of cells after seed inoculation. The gfp-tagged strain, MA 342G2, had the same biocontrol efficacy as the wild type when it was applied at high cell concentrations to seeds but was less effective at lower cell concentrations. By comparing cell counts determined by microscopy to the number of CFU, we found that the number of culturable cells was significantly lower than the total number of bacteria on seeds which were inoculated and dried for 20 h. Confocal microscopy and epifluorescence stereomicroscopy were used to determine the pattern of MA 342G2 colonization and cell aggregation on barley seeds. Immediately after inoculation of seeds, bacteria were found mainly under the seed glume, and there was no particular aggregation pattern. However, after the seeds were sown, irregularly distributed areas of bacterial aggregation were found, which reflected epiphytic colonization of glume cells. There was a trend towards bacterial aggregation near the embryo but never within the embryo. Bacterial aggregates were regularly found in the groove of each seed formed by the base of the coleoptile and the scutellum. Based on these results, we suggest that MA 342 colocalizes with the pathogen D. teres, which facilitates the action of the fungistatic compound(s) produced by this strain.

  19. Real-time Cosmology

    CERN Document Server

    Quercellini, Claudia; Balbi, Amedeo; Cabella, Paolo; Quartin, Miguel

    2010-01-01

    In recent years the possibility of measuring the temporal change of radial and transverse position of sources in the sky in real time have become conceivable thanks to the thoroughly improved technique applied to new astrometric and spectroscopic experiments, leading to the research domain we call Real-time cosmology. We review for the first time great part of the work done in this field, analysing both the theoretical framework and some endeavor to foresee the observational strategies and their capability to constrain models. We firstly focus on real time measurements of the overall redshift drift and angular separation shift in distant source, able to trace background cosmic expansion and large scale anisotropy, respectively. We then examine the possibility of employing the same kind of observations to probe peculiar and proper acceleration in clustered systems and therefore the gravitational potential. The last two sections are devoted to the short time future change of the cosmic microwave background, as ...

  20. Real-time radiography

    Energy Technology Data Exchange (ETDEWEB)

    Bossi, R.H.; Oien, C.T.

    1981-02-26

    Real-time radiography is used for imaging both dynamic events and static objects. Fluorescent screens play an important role in converting radiation to light, which is then observed directly or intensified and detected. The radiographic parameters for real-time radiography are similar to conventional film radiography with special emphasis on statistics and magnification. Direct-viewing fluoroscopy uses the human eye as a detector of fluorescent screen light or the light from an intensifier. Remote-viewing systems replace the human observer with a television camera. The remote-viewing systems have many advantages over the direct-viewing conditions such as safety, image enhancement, and the capability to produce permanent records. This report reviews real-time imaging system parameters and components.

  1. Real-Time Shading

    CERN Document Server

    Olano, Marc

    2002-01-01

    This book covers real-time shading systems, their design and how they work. Procedural shading, long valued for off-line rendering and production animation is now possible on interactive graphics hardware. These developments are important for areas such as game development, product design, and scientific visualization, among others. The authors include examples of techniques for achieving common effects efficiently in a real-time shading language ranging from full procedural shading on advanced specialized hardware to limited, yet surprisingly flexible shading on unextended OpenGL, to modern P

  2. Real-Time Evaluations

    Directory of Open Access Journals (Sweden)

    UNHCR

    2002-07-01

    Full Text Available A real-time evaluation (RTE is a timely, rapid andinteractive review of a fast evolving humanitarianoperation undertaken at an early phase. Its broadobjectives are to gauge the effectiveness and impactof a given UNHCR response and to ensure that itsfindings are used as an immediate catalyst fororganisational and operational change.

  3. Nitric oxide and reactive oxygen species mediate metabolic changes in barley seed embryo during germination

    Directory of Open Access Journals (Sweden)

    Zhenguo eMa

    2016-02-01

    Full Text Available The levels of nitric oxide (NO and reactive oxygen species (ROS, ATP/ADP ratios, reduction levels of ascorbate and glutathione, expression of the genes encoding proteins involved in metabolism of NO and activities of the enzymes involved in fermentation and in metabolism of NO and ROS were studied in the embryos of germinating seeds of two barley (Hordeum vulgare L. cultivars differing in dormancy level. The level of NO production continuously increased after imbibition while the level of nitrosylated SH-groups in proteins increased. This corresponded to the decrease of free SH-groups in proteins. At early stage of germination (0-48 h postimbibition the genes encoding class 1 phytoglobin (the protein scavenging NO and S-nitrosoglutathione reductase (scavenging S-nitrosoglutathione were markedly expressed. More dormant cultivar exhibited lower ATP/ADP and ascorbate/dehydroascorbate ratios and lower lactate and alcohol dehydrogenase activities, while the production of NO and nitrosylation of proteins was higher as compared to the non-dormant cultivar. The obtained data indicate that at the onset of germination NO is actively generated causing nitrosylation of SH-groups and a switch from respiration to fermentation. After radicle protrusion the metabolism changes in a more reducing type as recorded by ratio of reduced and oxidized glutathione and ascorbate. The turnover of NO by the scavenging systems (phytoglobin, S-nitrosoglutathione reductase and interaction with ROS might contribute to the maintenance of redox and energy balance of germinating seeds and lead to alleviation of dormancy.

  4. Real Time Econometrics

    OpenAIRE

    Pesaran, M. Hashem; Timmermann, Allan

    2004-01-01

    This paper considers the problems facing decision makers using econometric models in real time. It identifies the key stages involved and highlights the role of automated systems in reducing the effect of data snooping. It sets out many choices that researchers face in construction of automated systems and discusses some of the possible ways advanced in the literature for dealing with them. The role of feedbacks from the decision maker?s actions to the data generating process is also discusse...

  5. Real Time Processing

    CERN Document Server

    CERN. Geneva; ANDERSON, Dustin James; DOGLIONI, Caterina

    2015-01-01

    The LHC provides experiments with an unprecedented amount of data. Experimental collaborations need to meet storage and computing requirements for the analysis of this data: this is often a limiting factor in the physics program that would be achievable if the whole dataset could be analysed. In this talk, I will describe the strategies adopted by the LHCb, CMS and ATLAS collaborations to overcome these limitations and make the most of LHC data: data parking, data scouting, and real-time analysis.

  6. Construction of the Seed-Coat cDNA Microarray and Screening of Differentially Expressed Genes in Barley

    Institute of Scientific and Technical Information of China (English)

    Jin-Song PANG; Meng-Yuan HE; Bao LIU

    2004-01-01

    Some barley mutants can synthesize neither anthocyanins nor proanthocyanidins in the seed coat, which is related to several genes in locus Ant13, but the exact model of action remains unknown. We used the cDNA microarray technology with barley transcription-deficient mutant (ant13-152) that does not synthesize proanthocyanidins as the tester, and its wild type genotype (Triumph) as the driver, to study this question. Six-thousand and forty-eight clones from the wild type Morex testa+pericarp cDNA library were amplified using PCR, and the DNA fragments were spotted on commercial amino-modified glass slide as microarray. The mRNAs from the developing seed coat (8-15 days) of both the mutant and the wild-type barley plants were isolated, and labeled respectively with Cy3-dUTP and Cy5-dUTP when reversely transcribed to cDNAs. The labeled cDNAs were used as probes, mixed at the same molar concentration, and hybridized with the DNA fragments on the slide. Seventy clones exhibiting marked differential expression (ratio>4) were identified from the microarray. All the 25 cDNA clones that showed an over-expression in wild type in comparison to the mutant ant13-152 were sequenced. It was found that most of these overexpressing clones were transcription/translation and hordein-associated genes. These results have laid a solid material basis for further elucidation of the metabolic pathway in proanthocyanidin synthesis in barley and likely other plants.

  7. Optimization of the most important operational parameters of a pneumatic seeder using real-time monitoring for Cucumber and Watermelon seeds

    Directory of Open Access Journals (Sweden)

    Z Abdolahzare

    2016-04-01

    Full Text Available Introduction: Since the application of pneumatic planters for seeds with different physical properties is growing, it is essential to evaluation the performance of these machines to improve the operating parameters under different pressures and forward speeds. To evaluate the performance of precision vacuum seeders numerous procedures of laboratory and field have been developed and their feed mechanism evaluation is of great importance. The use of instrumentation is essential in laboratory procedures. Many systems have been designed, using instrumentation, to be able to monitor seed falling trajectory and as a result, in those systems the precise place of falling seed in the seed bed could be determined. In this study, the uniformity of seed spacing of a seed drill was determined using of high speed camera with a frame rate of 480 frames s-1. So that, the uniformity of planting was statistically significant under the influence of the speed of seed metering rollers (Karayel et al., 2006. Singh et al. (2005 studied the effects of disk rotation speed, vacuum pressure and shape of seed entrance hole on planting spacing uniformity using uniformity indices under laboratory and field conditions. They reported miss index values were reduced as the pressure was increased but they were increased with increasing of the speed. The multiple indices on the other hand were low at higher speed but they were increased as the pressure was increased. Ground speed was affected by changes in engine speed and gear selection, both of which effect on amount of fan rotation speed for different pressures. The aim of this study was to identify and determine the effects of forward speed and optimum vacuum pressure amount of the pneumatic seeder. Materials and Methods: The pneumatic planter (Unissem was mounted on a tractor (MF399 and passed over the soil bin. Thus, the acquired data would be more reliable and practical. To do so, the tractor was equipped with electronic

  8. Real time Faraday spectrometer

    Science.gov (United States)

    Smith, Jr., Tommy E.; Struve, Kenneth W.; Colella, Nicholas J.

    1991-01-01

    This invention uses a dipole magnet to bend the path of a charged particle beam. As the deflected particles exit the magnet, they are spatially dispersed in the bend-plane of the magnet according to their respective momenta and pass to a plurality of chambers having Faraday probes positioned therein. Both the current and energy distribution of the particles is then determined by the non-intersecting Faraday probes located along the chambers. The Faraday probes are magnetically isolated from each other by thin metal walls of the chambers, effectively providing real time current-versus-energy particle measurements.

  9. No effect of 5-fluorouracil on the properties of purified. cap alpha. -amylase from barley half-seeds

    Energy Technology Data Exchange (ETDEWEB)

    Rodaway, S.J.; Kende, H.

    1978-01-01

    Amylase has been purified from de-embryonated seeds of barley (Horedeum vulgare L. cv. Betzes) which have been incubated on 10/sup 6/M gibberellic acid (GA/sub 3/) following 3 days of imbibition in buffer. Incubation of the half-seeds in up to 10/sup -2/ M 5-fluorouracil (5-FU) during the entire incubation period, including imbibition, had no effect on any of the following characteristics of purified ..cap alpha..-amylase: thermal stability in the absence of calcium, molecular weight of the enzyme, isozyme composition, specific activity, or the amount of ..cap alpha..-amylase synthesized by the aleurone tissue. The synthesis of rRNA and tRNA was strongly inhibited by 5-FU, indicating that the analog had entered the aleurone cells. These results are not in agreement with those of Carlson (Nature New Biology 237: 39-41 (1972)) who found that treatment of barley aleurone with 10/sup -4/ M 5-FU to the addition of GA/sub 3/ resulted in decreased thermal stability of GA/sub 3/-induced ..cap alpha..-amylase and who interpreted this as evidence that the mRNA for ..cap alpha..-amylase was synthesized during the imbibition of the aleurone tissue and independently of gibberellin action. Results of the present experiments indicate that the thermal stability of highly purified ..cap alpha..-amylase is not altered by treatment of barley half-seeds with 5-FU, and that 5-FU cannot be used as a probe to examine the timing of ..cap alpha..-amylase mRNA synthesis.

  10. Real time production optimization

    Energy Technology Data Exchange (ETDEWEB)

    Saputelli, Luigi; Otavio, Joao; Araujo, Turiassu; Escorcia, Alvaro [Halliburton, Houston, TX (United States). Landmark Division

    2004-07-01

    Production optimization encompasses various activities of measuring, analyzing, modeling, prioritizing and implementing actions to enhance productivity of a field. We present a state-of-the-art framework for optimizing production on a continuous basis as new sensor data is acquired in real time. Permanently acquired data is modeled and analyzed in order to create predictive models. A model based control strategy is used to regulate well and field instrumentation. The optimum field operating point, which changes with time, satisfies the maximum economic return. This work is a starting point for further development in automatic, intelligent reservoir technologies which get the most out of the abilities of permanent, instrumented wells and remotely activated downhole completions. The strategy, tested with history-matched data from a compartmentalised giant field, proved to reduce operating costs while increasing oil recovery by 27% in this field. (author)

  11. Characterization of the microchemical structure of seed endosperm within a cellular dimension among six barley varieties with distinct degradation kinetics, using ultraspatially resolved synchrotron-based infrared microspectroscopy.

    Science.gov (United States)

    Liu, Na; Yu, Peiqiang

    2010-07-14

    Barley varieties have similar chemical composition but exhibit different rumen degradation kinetics and nutrient availability. These biological differences may be related to molecular, structural, and chemical makeup among the seed endosperm tissue. No detailed study was carried out. The objectives of this study were: (1) to use a molecular spectroscopy technique, synchrotron-based Fourier transform infrared microspectroscopy (SFTIRM), to determine the microchemical-structural features in seed endosperm tissue of six developed barley varieties; (2) to study the relationship among molecular-structural characteristics, degradation kinetics, and nutrient availability in six genotypes of barley. The results showed that inherent microchemical-structural differences in the endosperm among the six barley varieties were detected by the synchrotron-based analytical technique, SFTIRM, with the univariate molecular spectral analysis. The SFTIRM spectral profiles differed (P degradation). Weak correlations may indicate that limited variations of these six barley varieties might not be sufficient to interpret the relationship between spectroscopic information and the nutrient value of barley grain, although significant differences in biodegradation kinetics were observed. In conclusion, the studies demonstrated the potential of ultraspatially resolved synchrotron based technology (SFTIRM) to reveal the structural and chemical makeup within cellular and subcellular dimensions without destruction of the inherent structure of cereal grain tissue.

  12. Evaluation of phytotoxicity effect of olive mill wastewater treated by different technologies on seed germination of barley (Hordeum vulgare L.).

    Science.gov (United States)

    Rusan, Munir J M; Albalasmeh, Ammar A; Zuraiqi, Said; Bashabsheh, Mohammad

    2015-06-01

    Olive-mill wastewater (OMW) is a by-product effluent of olive oil extraction process that is produced in large amount in the Mediterranean region. OMW is believed to induce phytotoxic effect on organisms including seed germination and plant growth. The objective of this study was to evaluate the impact of untreated and treated OMW with different techniques on seed germination of barley (Hordeum vulgare L.). The following treatments were investigated: (1) tap water (control); (2) OMW treated by aerobic biological technology in a Jacto Reactor (JR); (3) OMW treated by solar fenton oxidation (SFO); (4) OMW treated by microfiltration followed by nanofiltration (MF+NF); (5) OMW treated by microfiltration followed by reverse osmosis (MF+RO) process; (6) diluted OMW with tap water (25 % OMW); (7) diluted OMW with tap water (50 % OMW); (8) diluted OMW with tap water (75 % OMW); and (9) untreated OMW (100 % OMW). A germination test was conducted in an incubator at temperature of 23 (∘)C. In each petri dish, a filter paper was mounted and ten seeds of barley were placed on the filter paper. Five milliliter of water were added to each petri dish. The seed germination was determined by counting the number of germinated seeds to calculate the percentage of germination (G %). Germination rate index (GRI), seed vigor index (SVI), and phytotoxicity index (PI) were also calculated. Then, the dry weights and lengths of the shoots and the roots of the germinated seeds were measured. The results show that 100, 75, and 50 %OMW were very phytotoxic and completely prohibited seed germination. However, phytotoxicity decreased significantly following treatments of OMW with all techniques investigated and by the 25 % OMW dilution, as results of removing the phenols and other phytotoxic organic compounds from the OMW or by diluting it. This was evidenced by relative enhancement of the dry weights and lengths of shoot and root as well as the G %, GRI, SVG, and PI. It was concluded that if

  13. Ovation Prime Real-Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Ovation Prime Real-Time (OPRT) product is a real-time forecast and nowcast model of auroral power and is an operational implementation of the work by Newell et...

  14. Two-dimensional gel electrophoresis pattern (pH 6-11) and identification of water-soluble barley seed and malt proteins by mass spectrometry

    DEFF Research Database (Denmark)

    Bak-Jensen, K.S.; Laugesen, S.; Roepstorff, P.

    2004-01-01

    A protocol was established for two-dimensional gel electrophoresis (2-DE) of barley seed and malt proteins in the pH range of 6-11. Proteins extracted from flour in a low-salt buffer were focused after cup-loading onto IPG strips. Successful separation in the second dimension was achieved using...

  15. Isolation and purification of a papain inhibitor from Egyptian genotypes of barley seeds and its in vitro and in vivo effects on the cowpea bruchid, Callosobruchus maculatus (F.).

    Science.gov (United States)

    Abd El-Latif, Ashraf Oukasha

    2015-02-01

    The cysteine inhibitors that are known as cystatin have been identified and characterized from several plant species. In the current study, 44 barley (Hordeum vulgare) genotypes including 3 varieties and 41 promising lines were screened for their potential as protease inhibitors. The barley genotypes showed low inhibitory activity against trypsin and chymotrypsin enzymes with a mean of 4.15 TIU/mg protein and 4.40 CIU/mg protein. The barley variety, Giza 123, showed strong papain inhibitory activity of 97.09 PIU/mg proteins and was subjected for further purification studies using ammonium sulfate fractionation and DEAE-Sephadex A-25 column. Barley purified proteins showed two bands on SDS-PAGE corresponding to a molecular mass of 12.4-54.8 kDa. The purified barley PI was found to be stable at a temperature below 80 °C and at a wide range of pH from 2 to 12. Barley PI was found to have higher potential inhibitory activity against papain enzyme compared to the standard papain inhibitor, E-64 with an IC50 value of 21.04 µg/ml and 25.62 µg/ml for barley PI and E-64, respectively. The kinetic analysis revealed a non-competitive type of inhibition with a Ki value of 1.95 × 10(-3 )µM. The antimetabolic effect of barley PI was evaluated against C. maculatus by incorporating the F30-60 protein of the purified inhibitor into the artificial diet using artificial seeds. Barley PI significantly prolonged the development of C. maculatus in proportion to PI concentration. Barley PI significantly increased the mortality of C. maculatus and caused a significant reduction in its fecundity. On the other hand, barley PI seemed to have non-significant effects on the adult longevity and the adult dry weight. The in vitro and in vivo results proved the efficiency of the papain inhibitory protein isolated from barley as a tool for managing the cowpea bruchid, C. maculatus.

  16. Production responses by early lactation cows to whole sunflower seed or tallow supplementation of a diet based on barley.

    Science.gov (United States)

    Markus, S B; Wittenberg, K M; Ingalls, J R; Undi, M

    1996-10-01

    A 2-yr study to evaluate the effectiveness of whole sunflower seed as a source of fat was conducted with 18 primiparous and 31 multiparous Holstein cows. The three diets evaluated were a basal diet based on barley (control), a basal diet supplemented with 2.7% tallow, and a basal diet supplemented with 7.1% whole sunflower seeds. The DMI of lactating cows during the 16-wk test period was not influenced by supplementation with either sunflower seeds or tallow. Milk production was 34.4, 34.6, and 35.5 kg/d for cows fed the control diet or the diets supplemented with sunflower or tallow, respectively, and was not influenced by diet. The production and concentrations of milk protein, fat, and SNF also were not influenced by diet. The concentrations of C6:0 to C14:1 fatty acids were highest in the milk of cows fed the control diet. The concentrations of C10:0 to C16:1 were higher when cows were fed the diet with the tallow supplement than when they were fed the diet with the sunflower supplement. However, the concentrations of C18:0 to C18:2 and C20:0 were higher in the milk of cows that were fed the sunflower supplement than in the milk of cows that were fed the tallow supplement or the control diet. Concentrations of individual VFA and the ratio of acetate to propionate were not influenced by diet. Body weight, body condition score, and reproduction parameters were similar for all diets, suggesting that there were no effects on subsequent production. The performance of cows fed whole sunflower seeds as a source of energy appeared to be similar to the performance of cows fed traditional high energy diets based on barley. The fatty acid profile of the milk of cows fed diets supplemented with sunflower seeds was more favorable than that of the milk of cows fed diets supplemented with tallow.

  17. Barley peroxidase isozymes. Expression and post-translational modification in mature seeds as identified by two-dimensional gel electrophoresis and mass spectrometry

    DEFF Research Database (Denmark)

    Laugesen, Sabrina; Bak-Jensen, Kristian Sass; Hägglund, Per

    2007-01-01

    putative barley peroxidase. The presence of multiple spots for each of the isozymes reflected variations in post-translational glycosylation and protein truncation. Complete sequence coverage was achieved by using a series of proteases and chromatographic resins for sample preparation prior to mass....... In summary, a subset of three peroxidase isozymes was found to occur in the seed, whereas products of four other barley peroxidase genes were not detected. The present analysis documents the selective expression profiles and post-translational modifications of isozymes from a large plant gene family. (C...

  18. ANALYSIS OF THE IMPACT OF PREPLANT TREATING OF SEEDS WITH INDUSTRIAL ALTERNATING ELECTROMAGNETIC FREQUENCY (EMF PF 50 HZ ON THE GERMINATION ENERGY OF MIDRIPENING SPRING BARLEY SEEDS OF THE VARIETIES OF VAKULA, VIKONT, RATNIK

    Directory of Open Access Journals (Sweden)

    Zholobova M. V.

    2016-04-01

    Full Text Available The use of physical factors for preplant stimulation of seeds increases vigor, germination and improves the yield of agricultural products. One of the effective ways to improve seed grain quality is to impact on the seeds using physical factors, in particular, an alternating electromagnetic field of industrial frequency of 50 Hz (EMF of IF of 50 Hz. We studied the impact of EMF of IF of 50 Hz on the energy germination of mid-ripening spring barley seeds of promising varieties Vakula, Vikont, Ratnik. Carrying out the experimental studies we used a laboratory setup with annular pole points of rectangular cross section. Our disperse analysis resulted in data on germinative energy of seeds of spring barley varieties Vakula, Vikont, Ratnik, which showed that while treating seeds of spring barley by an alternating electromagnetic field of industrial frequency of 50Hz, we obtained the best values of germinative energy of seeds of Variety Vikont with a mode of processing being equal to W = 16%, T = 2 c, L = 0,03 m

  19. A flexible loop controlling the enzymatic activity and specificity in a glycosyl hydrolase family 19 endochitinase from barley seeds

    DEFF Research Database (Denmark)

    Fukamizo, Tamo; Miyake, Ryoh; Tamura, Atsushi

    2009-01-01

    To examine the role of the loop structure consisting of residues 70-82 (70-82 loop) localized to + 3/4 subsite of the substrate binding cleft of a family GH-19 endochitinase from barley seeds, Trp72 and Trp82 were mutated, and the mutated enzymes (W72A, W82A, and W72A/W82A) were characterized...... more substantial in W82A than in W72A, but surprisingly the effects of the W82A/W72A double mutation were intermediate between those of the two single mutations. A molecular dynamics simulation of the wild type and the Trp-mutated enzymes indicated that the 70-82 loop becomes more flexible upon...

  20. Effect of cadmium on germination, coleoptile and root growth of barley seeds in the presence of gibberellic acid and kinetin.

    Science.gov (United States)

    Munzuroglu, Omer; Zengin, Fikriye Kirbag

    2006-10-01

    Effect of cadmium on barley seeds treated with kinetin and gibberellic acid was investigated. As usual, cadmium has inhibited seed germination, and showed important inhibitory effects on roots and coleoptile growth after germination. In general, increase in cadmium concentration caused a greater inhibition of germination, root and coleoptile growth. The adverse effect of cadmium on root and coleoptile growth was more pronounced than that on germination. While testa was pierced by radicle (an indication of germination), no root or coleoptile development was observed above at concentration of 3-9.5 mM CdCl2xH2O. Low concentrations of cadmium have inhibited the root growth more than it did on coleoptile growth. Treatment of seeds with gibberellic acid and kinetin did not show any significant difference on the effect of cadmium in germination. However, inhibition of coleoptile elongation by cadmium has decreased a very much after kinetin application. The same result, although with lower rates when compared to kinetin, has been obtained for GA3 as well. In addition, the inhibitory effect of cadmium on root growth increased even more after kinetin application. The results have been found statistically significant through the least significant different (LSD) test at levels ofp < 0.05 and p < 0.01.

  1. Designing Real Time Assistive Technologies

    DEFF Research Database (Denmark)

    Sonne, Tobias; Obel, Carsten; Grønbæk, Kaj

    2015-01-01

    design criteria in relation to three core components (sensing, recognizing, and assisting) for designing real time assistive technologies for children with ADHD. Based on these design criteria, we designed the Child Activity Sensing and Training Tool (CASTT), a real time assistive prototype that captures...... activities and assists the child in maintaining attention. From a preliminary evaluation of CASTT with 20 children in several schools, we and found that: 1) it is possible to create a wearable sensor system for children with ADHD that monitors physical and physiological activities in real time; and that 2......) real time assistive technologies have potential to assist children with ADHD in regaining attention in critical school situations....

  2. Designing Real Time Assistive Technologies

    DEFF Research Database (Denmark)

    Sonne, Tobias; Obel, Carsten; Grønbæk, Kaj

    design criteria in relation to three core components (sensing, recognizing, and assisting) for designing real time assistive technologies for children with ADHD. Based on these design criteria, we designed the Child Activity Sensing and Training Tool (CASTT), a real time assistive prototype that captures...... activities and assists the child in maintaining attention. From a preliminary evaluation of CASTT with 20 children in several schools, we and found that: 1) it is possible to create a wearable sensor system for children with ADHD that monitors physical and physiological activities in real time; and that 2......) real time assistive technologies have potential to assist children with ADHD in regaining attention in critical school situations....

  3. Effects of damping-off caused by Rhizoctonia solani anastomosis group 2-1 on roots of wheat and oil seed rape quantified using X-ray Computed Tomography and real-time PCR

    Directory of Open Access Journals (Sweden)

    Craig J. Sturrock

    2015-06-01

    Full Text Available Rhizoctonia solani is a plant pathogenic fungus that causes significant establishment and yield losses to several important food crops globally. This is the first application of high resolution X-ray micro Computed Tomography (X-ray µCT and real-time PCR to study host-pathogen interactions in situ and elucidate the mechanism of Rhizoctonia damping-off disease over a 6-day period caused by R. solani, anastomosis group (AG 2-1 in wheat (Triticum aestivum cv. Gallant and oil seed rape (OSR, Brassica napus cv. Marinka. Temporal, non-destructive analysis of root system architectures was performed using RooTrak and validated by the destructive method of root washing. Disease was assessed visually and related to pathogen DNA quantification in soil using real-time PCR. R. solani AG2-1 at similar initial DNA concentrations in soil was capable of causing significant damage to the developing root systems of both wheat and OSR. Disease caused reductions in primary root number, root volume, root surface area and convex hull which were affected less in the monocotyledonous host. Wheat was more tolerant to the pathogen, exhibited fewer symptoms and developed more complex root system. In contrast, R. solani caused earlier damage and maceration of the taproot of the dicot, OSR. Disease severity was related to pathogen DNA accumulation in soil only for OSR, however reductions in root traits were significantly associated with both disease and pathogen DNA. The method offers the first steps in advancing current understanding of soil-borne pathogen behaviour in situ at the pore scale, which may lead to the development of mitigation measures to combat disease influence in the field.

  4. Detection of Expression Intensity of Aspergillus flavus Resistance Gene in Peanut Seed Capsule by Quantitative Real -Time PCR%荧光量PCR技术检测花生种皮抗黄曲霉基因表达强度

    Institute of Scientific and Technical Information of China (English)

    张廷婷; 李春娟; 闫彩霞; 孙兵; 郑奕雄; 单世华

    2012-01-01

    依据已获得的基因芯片和cDNA研究结果,筛选上调表达并且表达差异系数在5.0以上的花生种皮基因序列设计引物,采用SYBR Green Ⅰ实时荧光定量PCR法对上述基因进行相对定量分析,以验证基因芯片研究结果并为分离黄曲霉抗性相关基因奠定基础.结果表明:荧光定量PCR的结果与基因芯片的结果高度一致,目的基因在感病和抗病品种之间差异显著,其中BE87、AW03在抗病品种中相对表达量较高,可能在抵御黄曲霉侵染过程中发挥重要作用.%According to the research results of obtained gene chip and cDNA library, the primers were designed based on the gene sequences of peanut seed capsule which up - regulated expression and the differential expression coefficient more than 5.0. The relative quantitative analysis of these genes was carried out by SYBR Green I quantitative real - time PCR to identify the research results of gene chip and provide foundation for separating related genes resistant to Aspergillus flavus. The results showed that the quantitative real -time PCR results were consistent with the research results of gene chip on a high degree; there were significant differences between Aspergillus flavus resistant and susceptible varieties. Among which, BES1 and j4WD3 expressed more in Aspergillus flavus resistant varieties and maybe play an important role in defense from Aspergillus flavus infection.

  5. Nucleotide sequence of a cDNA coding for the barley seed protein CMa: an inhibitor of insect α-amylase

    DEFF Research Database (Denmark)

    Rasmussen, Søren Kjærsgård; Johansson, A.

    1992-01-01

    The primary structure of the insect alpha-amylase inhibitor CMa of barley seeds was deduced from a full-length cDNA clone pc43F6. Analysis of RNA from barley endosperm shows high levels 15 and 20 days after flowering. The cDNA predicts an amino acid sequence of 119 residues preceded by a signal...... peptide of 25 amino acids. Ala and Leu account for 55% of the signal peptide. CMa is 60-85% identical with alpha-amylase inhibitors of wheat, but shows less than 50% identity to trypsin inhibitors of barley and wheat. The 10 Cys residues are located in identical positions compared to the cereal inhibitor...

  6. Antioxidant activity of 100% and 80% methanol extracts from barley seeds (Hordeum vulgare L.: stabilization of sunflower oil

    Directory of Open Access Journals (Sweden)

    Iqbal, Shahid

    2010-09-01

    Full Text Available The antioxidant potential of 100% and 80% methanol extracts from the seeds of three barley varieties (Jou 83, Jou 87 and Haider 93 was assessed. The extract yields from barley seeds ranged from 3.23% (Haider 93,100% methanol to 5.31% (Jou 83, 80% methanol. The total phenolic contents, DPPH radical scavenging activity (IC50 values and inhibition of linoleic acid oxidation of barley seed extracts (BSE were determined to be 88.1-145.7 mg/100g, 90.8-168.6 μg/mL and 62.6-74.6%, respectively. The antioxidant effectiveness of BSE was also assessed by stabilizing sunflower oil (SFO with BSE at a concentration of 600 ppm (oil weight basis. The stabilized (treated with extract and the control (without extract addition SFO samples were subjected to accelerated (oven heating at 60ºC for 30 days, 8 h heating cycle/day storage. These were analyzed at regular intervals for the extent of oxidative changes according to the measurements of their contents of peroxide value, para-anisidine value, conjugated dienes and conjugated trienes. Generally, the 80% methanol extract of barely seeds demonstrated better antioxidant action than the 100% methanol extract. The antioxidant activity of BSE was also found to be considerably varied among the varieties tested. The present results suggest that antioxidant extracts from barely seeds might be used to protect vegetable oils from oxidation.El potencial antioxidante de extractos de metanol al 100% y el 80% de semillas de tres variedades de cebada (Jou 83, Jou 87 y Haider 93 fue evaluada. El rendimiento de los extractos de las semillas de cebada vario desde un 3.23% (Haider, 100% methanol a un 5.31% (Jou 83, 80% metanol. El contenido total de fenoles, la actividad atrapadora del radical DPPH (valores IC50 y la inhibición de la oxidación del ácido linoleico de los extractos de semilla de cebada (BSE fueron 88.1-145.7 mg/100g, 90.8-168.6 μg/mL y 62.6- 74.6%, respectivamente. La efectividad antioxidante de BSE fue tambi

  7. Real-time volume graphics

    CERN Document Server

    Engel, Klaus; Kniss, Joe; Rezk-Salama, Christof; Weiskopf, Daniel

    2006-01-01

    Based on course notes of SIGGRAPH course teaching techniques for real-time rendering of volumetric data and effects; covers both applications in scientific visualization and real-time rendering. Starts with the basics (texture-based ray casting) and then improves and expands the algorithms incrementally. Book includes source code, algorithms, diagrams, and rendered graphics.

  8. Response of germinating barley seeds to Fusarium graminearum: The first molecular insight into Fusarium seedling blight

    DEFF Research Database (Denmark)

    Yang, Fen; Svensson, Birte; Finnie, Christine

    2011-01-01

    Fusarium seedling blight in cereals can result in significant reductions in plant establishment but has not received much attention. The disease often starts during seed germination due to sowing of the seeds infected by Fusarium spp. including Fusarium graminearum. In order to gain the first...... provides the first molecular insight into Fusarium seedling blight....

  9. Interrelation of exposure and exposure rate in germinating seeds of barley and its concurrence with dose-rate theory

    Energy Technology Data Exchange (ETDEWEB)

    Bottino, P.J.; Sparrow, A.H.; Schwemmer, S.S.; Thompson, K.H.

    1975-01-01

    Germinating seeds of barley were irradiated with /sup 137/Cs gamma rays at various combinations of total exposure (400-3200 R) and exposure rate (30-24,000 R/hr). Seedling height was measured 5 days after the initiation of irradiation and the various levels of growth inhibition produced by each combination of treatments were determined. The results obtained ranged from no effect on growth to 100 percent growth inhibition. Growth inhibition curves based on both total exposure and exposure rate were constructed. The exposures required to produce 20 and 35 percent growth inhibition at each exposure rate were determined, 35 percent growth inhibition being the highest level that could be determined over the entire range of rates used (20 percent growth inhibition was used for comparative purposes). For both levels of growth inhibition, as exposure rate increased (or, concomitantly, as exposure time decreased), the total exposure required to produce the end point decreased (effectiveness increased) as a straight line relationship on a double logarithmic plot between 30 and 1500 R/hr (0.03 to 0.3 hr exposure time). Above 1500 R/hr, further increases in exposure rate (or decreases in exposure time) increased the total exposure required for a given effect, i.e., effectiveness decreased. Conversion of exposure rate to exposure time demonstrates this point of change in effectiveness to occur well within one mitotic cycle. These results are discussed with regard to current dose-rate theory and are at least partially consistent therewith. A straight-line dependency of the exposure rate producing maximum growth inhibition on total exposure is shown. The point at which the combinations of exposure and exposure rate for 35 percent growth inhibition occurs is restricted to barley and may differ for other species. This may depend on chromosome size or DNA content and/or the mitotic cycle time characteristic of a species. (auth)

  10. Cy5 maleimide labelling for sensitive detection of free thiols in native protein extracts: identification of seed proteins targeted by barley thioredoxin h isoforms.

    Science.gov (United States)

    Maeda, Kenji; Finnie, Christine; Svensson, Birte

    2004-03-01

    Barley thioredoxin h isoforms HvTrxh1 and HvTrxh2 differ in temporal and spatial distribution and in kinetic properties. Target proteins of HvTrxh1 and HvTrxh2 were identified in mature seeds and in seeds after 72 h of germination. Improvement of the established method for identification of thioredoxin-targeted proteins based on two-dimensional electrophoresis and fluorescence labelling of thiol groups was achieved by application of a highly sensitive Cy5 maleimide dye and large-format two-dimensional gels, resulting in a 10-fold increase in the observed number of labelled protein spots. The technique also provided information about accessible thiol groups in the proteins identified in the barley seed proteome. In total, 16 different putative target proteins were identified from 26 spots using tryptic in-gel digestion, matrix-assisted laser-desorption ionization-time-of-flight MS and database search. HvTrxh1 and HvTrxh2 were shown to have similar target specificity. Barley alpha-amylase/subtilisin inhibitor, previously demonstrated to be reduced by both HvTrxh1 and HvTrxh2, was among the identified target proteins, confirming the suitability of the method. Several alpha-amylase/trypsin inhibitors, some of which are already known as target proteins of thioredoxin h, and cyclophilin known as a target protein of m-type thioredoxin were also identified. Lipid transfer protein, embryospecific protein, three chitinase isoenzymes, a single-domain glyoxalase-like protein and superoxide dismutase were novel identifications of putative target proteins, suggesting new physiological roles of thioredoxin h in barley seeds.

  11. Molecular cloning of the barley seed protein CMd: a variant member of the alpha-amylase/trypsin inhibitor family of cereals.

    Science.gov (United States)

    Halford, N G; Morris, N A; Urwin, P; Williamson, M S; Kasarda, D D; Lew, E J; Kreis, M; Shewry, P R

    1988-09-07

    The nucleotide and deduced amino-acid sequences of a cDNA clone encoding the barley seed protein CMd are described. The sequence is homologous with those of a family of inhibitors of alpha-amylase and trypsin, except for two short insertions. The longest of these (14 residues) is at the junction between the three proposed ancestral regions that comprise this family of proteins, and has limited identity with alpha-amylases of bacterial origin.

  12. Real-time vision systems

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, R.; Hernandez, J.E.; Lu, Shin-yee [Lawrence Livermore National Lab., CA (United States)

    1994-11-15

    Many industrial and defence applications require an ability to make instantaneous decisions based on sensor input of a time varying process. Such systems are referred to as `real-time systems` because they process and act on data as it occurs in time. When a vision sensor is used in a real-time system, the processing demands can be quite substantial, with typical data rates of 10-20 million samples per second. A real-time Machine Vision Laboratory (MVL) was established in FY94 to extend our years of experience in developing computer vision algorithms to include the development and implementation of real-time vision systems. The laboratory is equipped with a variety of hardware components, including Datacube image acquisition and processing boards, a Sun workstation, and several different types of CCD cameras, including monochrome and color area cameras and analog and digital line-scan cameras. The equipment is reconfigurable for prototyping different applications. This facility has been used to support several programs at LLNL, including O Division`s Peacemaker and Deadeye Projects as well as the CRADA with the U.S. Textile Industry, CAFE (Computer Aided Fabric Inspection). To date, we have successfully demonstrated several real-time applications: bullet tracking, stereo tracking and ranging, and web inspection. This work has been documented in the ongoing development of a real-time software library.

  13. Effects of Seed Priming on Root Characteristics of Two Barley (Hordeum vulgare L.) Cultivars in Different Levels of Salinity Stress by using Gel Chamber Technique

    OpenAIRE

    H. R Khazaei; A Nezami; B Saadatian; O Armand Pishe

    2015-01-01

    The study was conducted to evaluate the effect of seed priming on root growth of two barley cultivars (Hordeum vulgare L.) under salinity stress was performed in phytogel. The treatments included two cultivars of barely (Yusuf and Maquie), levels of seed priming (control, hydro-priming and priming with 4.35 g l-1 of urea) and four levels of NaCl salinity stress (0, 0.2, 0.3 M). The experiment was conducted in Factorial experiment based on a completely randomized design (CRD) with three replic...

  14. 26 kDa endochitinase from barley seeds: an interaction of the ionizable side chains essential for catalysis

    DEFF Research Database (Denmark)

    Ohnishi, Tsuneo; Juffer, André H; Tamoi, Masahiro;

    2005-01-01

    To explore the structure essential for the catalysis in 26 kDa endochitinase from barley seeds, we calculated theoretical pKa values of the ionizable groups based on the crystal structure, and then the roles of ionizable side chains located near the catalytic residue were examined by site...... coli expression system. The transition temperature of thermal unfolding (T(m)) of R215A was lower than that of the wild type protein by about 6.2 degrees C. In the crystal structure, the Arg215 side chain is in close proximity to the Glu203 side chain, whose theoretical pKa value was found...... to be abnormally low (-2.4), suggesting that these side chains may interact with each other. Mutation of Glu203 to alanine (E203A) completely eliminated the enzymatic activity and impaired the thermal stability (deltaT(m) = 6.4 degrees C) of the enzyme. Substrate binding ability was also affected by the Glu203...

  15. Real Time Sonic Boom Display

    Science.gov (United States)

    Haering, Ed

    2014-01-01

    This presentation will provide general information about sonic boom mitigation technology to the public in order to supply information to potential partners and licensees. The technology is a combination of flight data, atmospheric data and terrain information implemented into a control room real time display for flight planning. This research is currently being performed and as such, any results and conclusions are ongoing.

  16. Real time automatic scene classification

    NARCIS (Netherlands)

    Israël, Menno; Broek, van den Egon L.; Putten, van der Peter; Uyl, den Marten J.; Verbrugge, R.; Taatgen, N.; Schomaker, L.

    2004-01-01

    This work has been done as part of the EU VICAR (IST) project and the EU SCOFI project (IAP). The aim of the first project was to develop a real time video indexing classification annotation and retrieval system. For our systems, we have adapted the approach of Picard and Minka [3], who categorized

  17. 2015 nationwide survey revealed Barley stripe mosaic virus in Korean barley fields

    Science.gov (United States)

    A seed-transmitted virus has consistently caused significant economic damage to barley crops in Korea in recent years, and may be increasing because many farmers save seed for replanting. Because some barley seed is imported, there is the potential for introduction of new seed-transmitted viruses, c...

  18. ISTTOK real-time architecture

    Energy Technology Data Exchange (ETDEWEB)

    Carvalho, Ivo S., E-mail: ivoc@ipfn.ist.utl.pt; Duarte, Paulo; Fernandes, Horácio; Valcárcel, Daniel F.; Carvalho, Pedro J.; Silva, Carlos; Duarte, André S.; Neto, André; Sousa, Jorge; Batista, António J.N.; Hekkert, Tiago; Carvalho, Bernardo B.

    2014-03-15

    Highlights: • All real-time diagnostics and actuators were integrated in the same control platform. • A 100 μs control cycle was achieved under the MARTe framework. • Time-windows based control with several event-driven control strategies implemented. • AC discharges with exception handling on iron core flux saturation. • An HTML discharge configuration was developed for configuring the MARTe system. - Abstract: The ISTTOK tokamak was upgraded with a plasma control system based on the Advanced Telecommunications Computing Architecture (ATCA) standard. This control system was designed to improve the discharge stability and to extend the operational space to the alternate plasma current (AC) discharges as part of the ISTTOK scientific program. In order to accomplish these objectives all ISTTOK diagnostics and actuators relevant for real-time operation were integrated in the control system. The control system was programmed in C++ over the Multi-threaded Application Real-Time executor (MARTe) which provides, among other features, a real-time scheduler, an interrupt handler, an intercommunications interface between code blocks and a clearly bounded interface with the external devices. As a complement to the MARTe framework, the BaseLib2 library provides the foundations for the data, code introspection and also a Hypertext Transfer Protocol (HTTP) server service. Taking advantage of the modular nature of MARTe, the algorithms of each diagnostic data processing, discharge timing, context switch, control and actuators output reference generation, run on well-defined blocks of code named Generic Application Module (GAM). This approach allows reusability of the code, simplified simulation, replacement or editing without changing the remaining GAMs. The ISTTOK control system GAMs run sequentially each 100 μs cycle on an Intel{sup ®} Q8200 4-core processor running at 2.33 GHz located in the ATCA crate. Two boards (inside the ATCA crate) with 32 analog

  19. Identification of thioredoxin h-reducible disulphides in proteornes by differential labelling of cysteines: Insight into recognition and regulation of proteins in barley seeds by thioredoxin h

    DEFF Research Database (Denmark)

    Maeda, Kenji; Finnie, Christine; Svensson, Birte

    2005-01-01

    or production of recombinant proteins. Mass shifts of 15 peptides, induced by treatment with thioredoxin h and differential alkylation, identified specific reduction of nine disulphides in BASI, four alpha-amylase/trypsin inhibitors and a protein of unknown function. Two specific disulphides, located...... alpha-amylase/subtilisin inhibitor (BASI) by barley thioredoxin h isoform 1 was analysed. Furthermore, the method was coupled with two-dimensional electrophoresis for convenient thioredoxin h-reducible disulphide identification in barley seed extracts without the need for protein purification...... structurally close to the alpha-amylase binding surfaces of BASI and alpha-amylase inhibitor BMAI-1 were demonstrated to be reduced to a particularly high extent. For the first time, specificity of thioredoxin h for particular disulphide bonds is demonstrated, providing a basis to study structural aspects...

  20. Chloroindolyl-3-acetic Acid and its Methyl Ester Incorporation of 36Cl in Immature Seeds of Pea and Barley

    DEFF Research Database (Denmark)

    Engvild, Kjeld Christensen

    1974-01-01

    compounds besides Cl−. One compound, present in pea and probably in barley, cochromatographed with a mixture of 4- and 6-chloroindolyl-3-acetic acid methyl esters. Another, detected in pea, but probably not in barley, cochromatographed with a mixture of 4-and 6-chloroindolyl-3-acetic acids....

  1. Identification of thioredoxin h-reducible disulphides in proteomes by differential labelling of cysteines: insight into recognition and regulation of proteins in barley seeds by thioredoxin h.

    Science.gov (United States)

    Maeda, Kenji; Finnie, Christine; Svensson, Birte

    2005-04-01

    Using thiol-specific fluorescence labelling, over 30 putative target proteins of thioredoxin h with diverse structures and functions have been identified in seeds of barley and other plants. To gain insight at the structural level into the specificity of target protein reduction by thioredoxin h, thioredoxin h-reducible disulphide bonds in individual target proteins are identified using a novel strategy based on differential alkylation of cysteine thiol groups by iodoacetamide and 4-vinylpyridine. This method enables the accessible cysteine side chains in the thiol form (carbamidomethylated) to be distinguished from those inaccessible or disulphide bound form (pyridylethylated) according to the mass difference in the peptide mass maps obtained by matrix-assistend laser desorption/ionisation-time of flight mass spectrometry. Using this approach, in vitro reduction of disulphides in recombinant barley alpha-amylase/subtilisin inhibitor (BASI) by barley thioredoxin h isoform 1 was analysed. Furthermore, the method was coupled with two-dimensional electrophoresis for convenient thioredoxin h-reducible disulphide identification in barley seed extracts without the need for protein purification or production of recombinant proteins. Mass shifts of 15 peptides, induced by treatment with thioredoxin h and differential alkylation, identified specific reduction of nine disulphides in BASI, four alpha-amylase/trypsin inhibitors and a protein of unknown function. Two specific disulphides, located structurally close to the alpha-amylase binding surfaces of BASI and alpha-amylase inhibitor BMAI-1 were demonstrated to be reduced to a particularly high extent. For the first time, specificity of thioredoxin h for particular disulphide bonds is demonstrated, providing a basis to study structural aspects of the recognition mechanism and regulation of target proteins.

  2. Spatio-temporal changes in germination and radical elongation of barley seeds tracked by proteome analysis of dissected embryo, aleurone layer and endosperm tissues

    DEFF Research Database (Denmark)

    Bønsager, Birgit Christine; Finnie, Christine; Roepstorff, P.

    2007-01-01

    Germination of barley is accompanied by changes in water-soluble seed proteins. 2-DE was used to describe spatio-temporal proteome differences in dissected seed tissues associated with germination and the subsequent radicle elongation. Protein identification by MS enabled assignment of proteins...... protein and an ABA-induced protein, suggesting that these proteins are also involved in desiccation stress. Several redox-related proteins differed in spatio-temporal patterns at the end of germination and onset of radicle elongation. Notably, ascorbate peroxidase that was observed only in the embryo......, increased in abundance at 36 h PI. The surprisingly early changes seen in the protein profiles already 4 h after imbibition indicate that germination is programmed during seed maturation...

  3. Barley peroxidase isozymes

    Science.gov (United States)

    Laugesen, Sabrina; Bak-Jensen, Kristian Sass; Hägglund, Per; Henriksen, Anette; Finnie, Christine; Svensson, Birte; Roepstorff, Peter

    2007-12-01

    Thirteen peroxidase spots on two-dimensional gels were identified by comprehensive proteome analysis of the barley seed. Mass spectrometry tracked multiple forms of three different peroxidase isozymes: barley seed peroxidase 1, barley seed-specific peroxidase BP1 and a not previously identified putative barley peroxidase. The presence of multiple spots for each of the isozymes reflected variations in post-translational glycosylation and protein truncation. Complete sequence coverage was achieved by using a series of proteases and chromatographic resins for sample preparation prior to mass spectrometric analysis. Distinct peroxidase spot patterns divided the 16 cultivars tested into two groups. The distribution of the three isozymes in different seed tissues (endosperm, embryo, and aleurone layer) suggested the peroxidases to play individual albeit partially overlapping roles during germination. In summary, a subset of three peroxidase isozymes was found to occur in the seed, whereas products of four other barley peroxidase genes were not detected. The present analysis documents the selective expression profiles and post-translational modifications of isozymes from a large plant gene family.

  4. Deciphering Mineral Homeostasis in Barley Seed Transfer Cells at Transcriptional Level.

    Directory of Open Access Journals (Sweden)

    Behrooz Darbani

    Full Text Available In addition to the micronutrient inadequacy of staple crops for optimal human nutrition, a global downtrend in crop-quality has emerged from intensive breeding for yield. This trend will be aggravated by elevated levels of the greenhouse gas carbon dioxide. Therefore, crop biofortification is inevitable to ensure a sustainable supply of minerals to the large part of human population who is dietary dependent on staple crops. This requires a thorough understanding of plant-mineral interactions due to the complexity of mineral homeostasis. Employing RNA sequencing, we here communicate transfer cell specific effects of excess iron and zinc during grain filling in our model crop plant barley. Responding to alterations in mineral contents, we found a long range of different genes and transcripts. Among them, it is worth to highlight the auxin and ethylene signaling factors Arfs, Abcbs, Cand1, Hps4, Hac1, Ecr1, and Ctr1, diurnal fluctuation components Sdg2, Imb1, Lip1, and PhyC, retroelements, sulfur homeostasis components Amp1, Hmt3, Eil3, and Vip1, mineral trafficking components Med16, Cnnm4, Aha2, Clpc1, and Pcbps, and vacuole organization factors Ymr155W, RabG3F, Vps4, and Cbl3. Our analysis introduces new interactors and signifies a broad spectrum of regulatory levels from chromatin remodeling to intracellular protein sorting mechanisms active in the plant mineral homeostasis. The results highlight the importance of storage proteins in metal ion toxicity-resistance and chelation. Interestingly, the protein sorting and recycling factors Exoc7, Cdc1, Sec23A, and Rab11A contributed to the response as well as the polar distributors of metal-transporters ensuring the directional flow of minerals. Alternative isoform switching was found important for plant adaptation and occurred among transcripts coding for identical proteins as well as transcripts coding for protein isoforms. We also identified differences in the alternative-isoform preference between

  5. Radiation damping in real time.

    Science.gov (United States)

    Mendes, A C; Takakura, F I

    2001-11-01

    We study the nonequilibrium dynamics of a charge interacting with its own radiation, which originates the radiation damping. The real-time equation of motion for the charge and the associated Langevin equation is found in classical limit. The equation of motion for the charge allows one to obtain the frequency-dependent coefficient of friction. In the lowest order we find that although the coefficient of static friction vanishes, there is dynamical dissipation represented by a non-Markovian dissipative kernel.

  6. Real-time flutter analysis

    Science.gov (United States)

    Walker, R.; Gupta, N.

    1984-01-01

    The important algorithm issues necessary to achieve a real time flutter monitoring system; namely, the guidelines for choosing appropriate model forms, reduction of the parameter convergence transient, handling multiple modes, the effect of over parameterization, and estimate accuracy predictions, both online and for experiment design are addressed. An approach for efficiently computing continuous-time flutter parameter Cramer-Rao estimate error bounds were developed. This enables a convincing comparison of theoretical and simulation results, as well as offline studies in preparation for a flight test. Theoretical predictions, simulation and flight test results from the NASA Drones for Aerodynamic and Structural Test (DAST) Program are compared.

  7. Real time analysis under EDS

    Energy Technology Data Exchange (ETDEWEB)

    Schneberk, D.

    1985-07-01

    This paper describes the analysis component of the Enrichment Diagnostic System (EDS) developed for the Atomic Vapor Laser Isotope Separation Program (AVLIS) at Lawrence Livermore National Laboratory (LLNL). Four different types of analysis are performed on data acquired through EDS: (1) absorption spectroscopy on laser-generated spectral lines, (2) mass spectrometer analysis, (3) general purpose waveform analysis, and (4) separation performance calculations. The information produced from this data includes: measures of particle density and velocity, partial pressures of residual gases, and overall measures of isotope enrichment. The analysis component supports a variety of real-time modeling tasks, a means for broadcasting data to other nodes, and a great degree of flexibility for tailoring computations to the exact needs of the process. A particular data base structure and program flow is common to all types of analysis. Key elements of the analysis component are: (1) a fast access data base which can configure all types of analysis, (2) a selected set of analysis routines, (3) a general purpose data manipulation and graphics package for the results of real time analysis. Each of these components are described with an emphasis upon how each contributes to overall system capability. 3 figs.

  8. Real-time analysis keratometer

    Science.gov (United States)

    Adachi, Iwao P. (Inventor); Adachi, Yoshifumi (Inventor); Frazer, Robert E. (Inventor)

    1987-01-01

    A computer assisted keratometer in which a fiducial line pattern reticle illuminated by CW or pulsed laser light is projected on a corneal surface through lenses, a prismoidal beamsplitter quarterwave plate, and objective optics. The reticle surface is curved as a conjugate of an ideal corneal curvature. The fiducial image reflected from the cornea undergoes a polarization shift through the quarterwave plate and beamsplitter whereby the projected and reflected beams are separated and directed orthogonally. The reflected beam fiducial pattern forms a moire pattern with a replica of the first recticle. This moire pattern contains transverse aberration due to differences in curvature between the cornea and the ideal corneal curvature. The moire pattern is analyzed in real time by computer which displays either the CW moire pattern or a pulsed mode analysis of the transverse aberration of the cornea under observation, in real time. With the eye focused on a plurality of fixation points in succession, a survey of the entire corneal topography is made and a contour map or three dimensional plot of the cornea can be made as a computer readout in addition to corneal radius and refractive power analysis.

  9. Autonomous Real Time Requirements Tracing

    Science.gov (United States)

    Plattsmier, George; Stetson, Howard

    2014-01-01

    One of the more challenging aspects of software development is the ability to verify and validate the functional software requirements dictated by the Software Requirements Specification (SRS) and the Software Detail Design (SDD). Insuring the software has achieved the intended requirements is the responsibility of the Software Quality team and the Software Test team. The utilization of Timeliner-TLX(sup TM) Auto- Procedures for relocating ground operations positions to ISS automated on-board operations has begun the transition that would be required for manned deep space missions with minimal crew requirements. This transition also moves the auto-procedures from the procedure realm into the flight software arena and as such the operational requirements and testing will be more structured and rigorous. The autoprocedures would be required to meet NASA software standards as specified in the Software Safety Standard (NASASTD- 8719), the Software Engineering Requirements (NPR 7150), the Software Assurance Standard (NASA-STD-8739) and also the Human Rating Requirements (NPR-8705). The Autonomous Fluid Transfer System (AFTS) test-bed utilizes the Timeliner-TLX(sup TM) Language for development of autonomous command and control software. The Timeliner-TLX(sup TM) system has the unique feature of providing the current line of the statement in execution during real-time execution of the software. The feature of execution line number internal reporting unlocks the capability of monitoring the execution autonomously by use of a companion Timeliner-TLX(sup TM) sequence as the line number reporting is embedded inside the Timeliner-TLX(sup TM) execution engine. This negates I/O processing of this type data as the line number status of executing sequences is built-in as a function reference. This paper will outline the design and capabilities of the AFTS Autonomous Requirements Tracker, which traces and logs SRS requirements as they are being met during real-time execution of the

  10. Real-time flood forecasting

    Science.gov (United States)

    Lai, C.; Tsay, T.-K.; Chien, C.-H.; Wu, I.-L.

    2009-01-01

    Researchers at the Hydroinformatic Research and Development Team (HIRDT) of the National Taiwan University undertook a project to create a real time flood forecasting model, with an aim to predict the current in the Tamsui River Basin. The model was designed based on deterministic approach with mathematic modeling of complex phenomenon, and specific parameter values operated to produce a discrete result. The project also devised a rainfall-stage model that relates the rate of rainfall upland directly to the change of the state of river, and is further related to another typhoon-rainfall model. The geographic information system (GIS) data, based on precise contour model of the terrain, estimate the regions that were perilous to flooding. The HIRDT, in response to the project's progress, also devoted their application of a deterministic model to unsteady flow of thermodynamics to help predict river authorities issue timely warnings and take other emergency measures.

  11. REAL TIME DATA PROCESSING FRAMEWORKS

    Directory of Open Access Journals (Sweden)

    Yash Sakaria

    2015-09-01

    Full Text Available On a business level, everyone wants to get hold of the business value and other organizational advantages that big data has to offer. Analytics has arisen as the primitive path to business value from big data. Hadoop is not just a storage platform for big data; it’s also a computational and processing platform for business analytics. Hadoop is, however, unsuccessful in fulfilling business requirements when it comes to live data streaming. The initial architecture of Apache Hadoop did not solve the problem of live stream data mining. In summary, the traditional approach of big data being co-relational to Hadoop is false; focus needs to be given on business value as well. Data Warehousing, Hadoop and stream processing complement each other very well. In this paper, we have tried reviewing a few frameworks and products which use real time data streaming by providing modifications to Hadoop.

  12. Triple Hybridization with Cultivated Barley (Hordeum vulgare L.)

    DEFF Research Database (Denmark)

    Bothmer, R. von; Claesson, L.; Flink, J.;

    1989-01-01

    represented species closely or distantly related to H. jubatum and H. lechleri. In trispecific crosses with diploid barley, the seed set was 5.7%. Crosses with tetraploid barley were highly unsuccessful (0.2% seed set). Three lines of diploid barley were used in the crosses, i.e. 'Gull', 'Golden Promise...

  13. A chemometric evaluation of the underlying physical and chemical patterns that support near infrared spectroscopy of barley seeds as a tool for explorative classification of endosperm genes and gene combinations

    DEFF Research Database (Denmark)

    Jacobsen, Susanne; Søndergaard, Ib; Møller, Birthe

    2005-01-01

    Near infrared spectroscopic (NIR; 1100-2500 nm), chemical and genetic data were combined to study the pleiotropic secondary effects of mutant genes on milled samples in a barley seed model. NIR and chemical data were both effective in classifying gene and gene combinations by Principal Component ...

  14. Clinical virology in real time.

    Science.gov (United States)

    Niesters, Hubert G M

    2002-12-01

    The ability to detect nucleic acids has had and still has a major impact on diagnostics in clinical virology. Both quantitative and qualitative techniques, whether signal or target amplification based systems, are currently used routinely in most if not all virology laboratories. Technological improvements, from automated sample isolation to real time amplification technology, have given the ability to develop and introduce systems for most viruses of clinical interest, and to obtain clinical relevant information needed for optimal antiviral treatment options. Both polymerase chain reaction (PCR) and nucleic acid sequence-based amplification (NASBA) can currently be used together with real time detection to generate results in a short turn-around time and to determine whether variants relevant for antiviral resistance are present. These new technologies enable the introduction of an individual patient disease management concept. Within our clinical setting, we have introduced this e.g. for quantitative detection of Epstein-Barr Virus (EBV) in T-dell depleted allogeneic stem cell transplant patients. This enabled us to develop models for pre-emptive anti B-cell immunotherapy for EBV reactivation, thereby effectively reducing not the incidence of EBV-lymphoproliferative disease but the virus related mortality. Furthermore, additional clinically relevant viruses can now easily be detected simultaneously. It also becomes more feasible to introduce molecular testing for those viruses that can easily be detected using classical virological methods, like culture techniques or antigen detection. Prospective studies are needed to evaluate the clinical importance of the additional positive samples detected. It should however be made clear that a complete exchange of technologies is unlikely to occur, and that some complementary technologies should stay operational enabling the discovery of new viruses. The implementation of these molecular diagnostic technologies furthermore

  15. Mobile real time radiography system

    Energy Technology Data Exchange (ETDEWEB)

    Vigil, J.; Taggart, D.; Betts, S. [Los Alamos National Lab., NM (United States)] [and others

    1997-11-01

    A 450-keV Mobile Real Time Radiography (RTR) System was delivered to Los Alamos National Laboratory (LANL) in January 1996. It was purchased to inspect containers of radioactive waste produced at (LANL). Since its delivery it has been used to radiograph more than 600 drums of radioactive waste at various LANL sites. It has the capability of inspecting waste containers of various sizes from <1-gal. buckets up to standard waste boxes (SWB, dimensions 54.5 in. x 71 in. x 37 in.). It has three independent x-ray acquisition formats. The primary system used is a 12- in. image intensifier, the second is a 36-in. linear diode array (LDA) and the last is an open system. It is fully self contained with on board generator, HVAC, and a fire suppression system. It is on a 53-ft long x 8-ft. wide x 14-ft. high trailer that can be moved over any highway requiring only an easily obtainable overweight permit because it weights {approximately}38 tons. It was built to conform to industry standards for a cabinet system which does not require an exclusion zone. The fact that this unit is mobile has allowed us to operate where the waste is stored, rather than having to move the waste to a fixed facility.

  16. Can a genetic correlation with seed mass constrain adaptive evolution of seedling desiccation tolerance in wild barley?

    NARCIS (Netherlands)

    Verhoeven, K.J.F.; Biere, A.; Nevo, E.; Van Damme, J.M.M.

    2004-01-01

    Very young seedlings of wild barley Hordeum spontaneum have the ability to survive extended periods of severe drought. This desiccation tolerance is considered an adaptation to the rain-limited and unpredictable habitats that the species occupies. Genetic variation has been observed for this trait,

  17. Microfluidic monitoring of programmed cell death in living plant seed tissue

    DEFF Research Database (Denmark)

    Mark, Christina; Heiskanen, Arto; Zor, Kinga;

    such as concentration of selected compounds, external pH, oxygen consumption, redox state and cell viability. The aleurone layer of the barley seed is a 2-3 single cell type thick tissue that can be dissected from the embryo and starchy endosperm. During incubation in vitro this mechanically very robust maintains......, 126, p. 156; Finnie, Christine, et al., (2011), Proteomics, 11, p. 1595). The potential of microfluidics real-time monitoring is relatively unexplored within plant biology, and the barley aleurone layer system will thus enable new ground to be broken in the field of plant science and microfluidics....

  18. Space Weather and Real-Time Monitoring

    OpenAIRE

    2009-01-01

    Recent advance of information and communications technology enables to collect a large amount of ground-based and space-based observation data in real-time. The real-time data realize nowcast of space weather. This paper reports a history of space weather by the International Space Environment Service (ISES) in association with the International Geophysical Year (IGY) and importance of real-time monitoring in space weather.

  19. Real-time Pricing in Power Markets

    DEFF Research Database (Denmark)

    Boom, Anette; Schwenen, Sebastian

    We examine welfare eects of real-time pricing in electricity markets. Before stochastic energy demand is known, competitive retailers contract with nal consumers who exogenously do not have real-time meters. After demand is realized, two electricity generators compete in a uniform price auction...... to satisfy demand from retailers acting on behalf of subscribed customers and from consumers with real-time meters. Increasing the number of consumers on real-time pricing does not always increase welfare since risk-averse consumers dislike uncertain and high prices arising through market power...

  20. Real-time Pricing in Power Markets

    DEFF Research Database (Denmark)

    Boom, Anette; Schwenen, Sebastian

    We examine welfare e ects of real-time pricing in electricity markets. Before stochastic energy demand is known, competitive retailers contract with nal consumers who exogenously do not have real-time meters. After demand is realized, two electricity generators compete in a uniform price auction...... to satisfy demand from retailers acting on behalf of subscribed customers and from consumers with real-time meters. Increasing the number of consumers on real-time pricing does not always increase welfare since risk-averse consumers dislike uncertain and high prices arising through market power...

  1. Real-time graphics rendering engine

    CERN Document Server

    Bao, Hujun

    2011-01-01

    ""Real-Time Graphics Rendering Engine"" reveals the software architecture of the modern real-time 3D graphics rendering engine and the relevant technologies based on the authors' experience developing this high-performance, real-time system. The relevant knowledge about real-time graphics rendering such as the rendering pipeline, the visual appearance and shading and lighting models are also introduced. This book is intended to offer well-founded guidance for researchers and developers who are interested in building their own rendering engines. Hujun Bao is a professor at the State Key Lab of

  2. Research in Distributed Real-Time Systems

    Science.gov (United States)

    Mukkamala, R.

    1997-01-01

    This document summarizes the progress we have made on our study of issues concerning the schedulability of real-time systems. Our study has produced several results in the scalability issues of distributed real-time systems. In particular, we have used our techniques to resolve schedulability issues in distributed systems with end-to-end requirements. During the next year (1997-98), we propose to extend the current work to address the modeling and workload characterization issues in distributed real-time systems. In particular, we propose to investigate the effect of different workload models and component models on the design and the subsequent performance of distributed real-time systems.

  3. Integration of the barley genetic and seed proteome maps for chromosome 1H, 2H, 3H, 5H and 7H.

    Science.gov (United States)

    Finnie, Christine; Bagge, Merethe; Steenholdt, Torben; Østergaard, Ole; Bak-Jensen, Kristian Sass; Backes, Gunter; Jensen, Anaïs; Giese, Henriette; Larsen, Jørgen; Roepstorff, Peter; Svensson, Birte

    2009-02-01

    Two-dimensional gel electrophoresis was used to screen spring barley cultivars for differences in seed protein profiles. In parallel, 72 microsatellite (simple sequence repeat (SSR)) markers and 11 malting quality parameters were analysed for each cultivar. Over 60 protein spots displayed cultivar variation, including peroxidases, serpins and proteins with unknown functions. Cultivars were clustered based on the spot variation matrix. Cultivars with superior malting quality grouped together, indicating malting quality to be more closely correlated with seed proteomes than with SSR profiles. Mass spectrometry showed that some spot variations were caused by amino acid differences encoded by single nucleotide polymorphisms (SNPs). Coding SNPs were validated by mass spectrometry, expressed sequence tag and 2D gel data. Coding SNPs can alter function of affected proteins and may thus represent a link between cultivar traits, proteome and genome. Proteome analysis of doubled haploid lines derived from a cross between a malting (Scarlett) and a feed cultivar (Meltan) enabled genetic localisation of protein phenotypes represented by 48 spot variations, involving e.g. peroxidases, serpins, alpha-amylase/trypsin inhibitors, peroxiredoxin and a small heat shock protein, in relation to markers on the chromosome map.

  4. The improved broadband Real-Time Seismic Network in Romania

    Science.gov (United States)

    Neagoe, C.; Ionescu, C.

    2009-04-01

    Starting with 2002 the National Institute for Earth Physics (NIEP) has developed its real-time digital seismic network. This network consists of 96 seismic stations of which 48 broad band and short period stations and two seismic arrays are transmitted in real-time. The real time seismic stations are equipped with Quanterra Q330 and K2 digitizers, broadband seismometers (STS2, CMG40T, CMG 3ESP, CMG3T) and strong motions sensors Kinemetrics episensors (+/- 2g). SeedLink and AntelopeTM (installed on MARMOT) program packages are used for real-time (RT) data acquisition and exchange. The communication from digital seismic stations to the National Data Center in Bucharest is assured by 5 providers (GPRS, VPN, satellite communication, radio lease line and internet), which will assure the back-up communications lines. The processing centre runs BRTT's AntelopeTM 4.10 data acquisition and processing software on 2 workstations for real-time processing and post processing. The Antelope Real-Time System is also providing automatic event detection, arrival picking, event location and magnitude calculation. It provides graphical display and reporting within near-real-time after a local or regional event occurred. Also at the data center was implemented a system to collect macroseismic information using the internet on which macro seismic intensity maps are generated. In the near future at the data center will be install Seiscomp 3 data acquisition processing software on a workstation. The software will run in parallel with Antelope software as a back-up. The present network will be expanded in the near future. In the first half of 2009 NIEP will install 8 additional broad band stations in Romanian territory, which also will be transmitted to the data center in real time. The Romanian Seismic Network is permanently exchanging real -time waveform data with IRIS, ORFEUS and different European countries through internet. In Romania, magnitude and location of an earthquake are now

  5. Real-time communication protocols: an overview

    NARCIS (Netherlands)

    Hanssen, Ferdy; Jansen, Pierre G.

    2003-01-01

    This paper describes several existing data link layer protocols that provide real-time capabilities on wired networks, focusing on token-ring and Carrier Sense Multiple Access based networks. Existing modifications to provide better real-time capabilities and performance are also described. Finally

  6. Real-time communication protocols: an overview

    OpenAIRE

    Hanssen, Ferdy; Jansen, Pierre G.

    2003-01-01

    This paper describes several existing data link layer protocols that provide real-time capabilities on wired networks, focusing on token-ring and Carrier Sense Multiple Access based networks. Existing modifications to provide better real-time capabilities and performance are also described. Finally the pros and cons regarding the At-Home Anywhere project are discussed.

  7. Validation of RNAi by real time PCR

    DEFF Research Database (Denmark)

    Josefsen, Knud; Lee, Ying Chiu

    2011-01-01

    Real time PCR is the analytic tool of choice for quantification of gene expression, while RNAi is concerned with downregulation of gene expression. Together, they constitute a powerful approach in any loss of function studies of selective genes. We illustrate here the use of real time PCR to veri...

  8. Storm real-time processing cookbook

    CERN Document Server

    Anderson, Quinton

    2013-01-01

    A Cookbook with plenty of practical recipes for different uses of Storm.If you are a Java developer with basic knowledge of real-time processing and would like to learn Storm to process unbounded streams of data in real time, then this book is for you.

  9. Real-time Pricing in Power Markets

    DEFF Research Database (Denmark)

    Boom, Anette; Schwenen, Sebastian

    to satisfy demand from retailers acting on behalf of subscribed customers and from consumers with real-time meters. Increasing the number of consumers on real-time pricing does not always increase welfare since risk-averse consumers dislike uncertain and high prices arising through market power...

  10. Interactive Real-time Magnetic Resonance Imaging

    DEFF Research Database (Denmark)

    Brix, Lau

    Real-time acquisition, reconstruction and interactively changing the slice position using magnetic resonance imaging (MRI) have been possible for years. However, the current clinical use of interactive real-time MRI is limited due to an inherent low spatial and temporal resolution. This PhD proje...

  11. THE STUDY OF THE INFLUENCE OF AN ALTERNATING ELECTROMAGNETIC FIELD OF COMMERCIAL FREQUENCY ON CROP SEED QUALITY OF SPRING BARLEY

    OpenAIRE

    Zholobova M. V.; Fedorishchenko M. G.; Shabanov N. I.; Grachyova N. N.

    2015-01-01

    The process of the presowing seed treatment with alternating electromagnet field of the commercial frequency (EMF CF) 50 Hz is economically efficient. The use of the EMF CF 50 Hz in the unit with ring pole pieces allows increasing the germinating energy and power. The electromagnet seed treatment in the units with ring pole pieces is the perspective process of the presowing treatment which does not make unhealthy influence on the operating personnel. At the presowing treatment we have to take...

  12. The ALMA Real Time Control System

    Science.gov (United States)

    Kern, Jeffrey S.; Juerges, Thomas A.; Marson, Ralph G.

    2009-01-01

    The Atacama Large Millimeter Array (ALMA) is a revolutionary millimeter and submillimeter array being developed on the Atacama plateau of northern Chile. An international partnership lead by NRAO, ESO, and NAOJ this powerful and flexible telescope will provide unprecedented observations of this relatively unexplored frequency range. The control subsystem for the Atacama Large Millimeter Array must coordinate the monitor and control of at least sixty six antennas (in four different styles), two correlators, and all of the ancillary equipment (samplers, local oscillators, front ends, etc.). This equipment will be spread over tens of kilometers and operated remotely. Operation of the array requires a robust, scalable, and maintainable real time control system. The real time control system is responsible for monitoring and control of any devices where there are fixed deadlines. Examples in the ALMA context are antenna pointing and fringe tracking. Traditionally the real time portion of a large software system is an intricate and error prone portion of the software. As a result the real time portion is very expensive in terms of effort expended both during construction and during maintenance phases of a project. The ALMA real time control system uses a Linux based real time operating system to interact with the hardware and the CORBA based ALMA Common Software to communicate in the distributed computing environment. Mixing the requirements of real time computing and the non-deterministic CORBA middleware has produced an interesting design. We discuss the architecture, design, and implementation of the ALMA real time control system. Highlight some lessons learned along the way, and justify our assertion that this should be the last large scale real time control system in radio astronomy.

  13. Real time detecting system for turning force

    CERN Document Server

    Yue Xiao Bin

    2001-01-01

    How to get the real-time value of forces dropped on the tool in the course of processing by piezoelectric sensors is introduced. First, the analog signals of the cutting force were achieved by these sensors, amplified and transferred into digital signals by A/D transferring card. Then real-time software reads the information, put it into its own coordinate, drew the curve of forces, displayed it on the screen by the real time and saved it for the technicians to analyze the situation of the tool. So the cutting parameter can be optimized to improve surface quality of the pieces

  14. Real-time medical applications and telecommunications.

    Science.gov (United States)

    Stravs, M

    1999-01-01

    Telecommunications play an important role in telemedicine. Many forms of telecommunication services based on different telecommunication technologies are developed for various needs. The paper deals with complex real-time applications which demand high telecommunication requirements. At the beginning, medical applications are categorised and real-time applications qualified as multimedia applications. Requirements for multimedia elements are listed separately. Later on, short introduction of related telecommunication protocols is given. Real-time medical applications can show their ability in case of guaranteed quality of services delivered by telecommunication network as it is explained in the end.

  15. Real-Time DMB Video Encryption in Recording on PMP

    Directory of Open Access Journals (Sweden)

    Seong-Yeon Lee

    2009-03-01

    Full Text Available At July 2008, the nationwide DMB (Digital Multimedia Broadcasting has got started. As DMB device's volume of sales has been over 12 million already, the development of rapidly changing network systems has increased the dangers of infringing the copyright of DMB contents. For solving this problem, we suggest that real-time encryption algorithm without additional expenses, and implemented that on PMP. We used the SEED algorithm for encryption. As the experimental result, we confirmed that the real-time encryption and decryption of DMB videos were working well. Also confirmed that recorded contents in PMP were not replayed in other devices, and also the contents recorded by other devices were not played in PMP. Suggested algorithms can be useful in the VOD, IPTV or cable TV.

  16. Real Time Study and Related Variables

    Institute of Scientific and Technical Information of China (English)

    WU Xiao-qing

    2016-01-01

    This paper first, illustrates the advantages of applying real time study to linguistic researches. Second, this paper also compares linguistic variables with linguistic variant; nasality, stronger constraint and weaker constraint have been clearly de-fined as well.

  17. Multiprocessor scheduling for real-time systems

    CERN Document Server

    Baruah, Sanjoy; Buttazzo, Giorgio

    2015-01-01

    This book provides a comprehensive overview of both theoretical and pragmatic aspects of resource-allocation and scheduling in multiprocessor and multicore hard-real-time systems.  The authors derive new, abstract models of real-time tasks that capture accurately the salient features of real application systems that are to be implemented on multiprocessor platforms, and identify rules for mapping application systems onto the most appropriate models.  New run-time multiprocessor scheduling algorithms are presented, which are demonstrably better than those currently used, both in terms of run-time efficiency and tractability of off-line analysis.  Readers will benefit from a new design and analysis framework for multiprocessor real-time systems, which will translate into a significantly enhanced ability to provide formally verified, safety-critical real-time systems at a significantly lower cost.

  18. Visualization in Real-Time Experiment Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The objective of this project will be to migrate some of the outputs from the WFF Mission Planning Lab (MPL) into a real-time visualization system.  The MPL is...

  19. Scala for Real-Time Systems?

    DEFF Research Database (Denmark)

    Schoeberl, Martin

    2015-01-01

    Java served well as a general-purpose language. However, during its two decades of constant change it has gotten some weight and legacy in the language syntax and the libraries. Furthermore, Java's success for real-time systems is mediocre. Scala is a modern object-oriented and functional language...... with interesting new features. Although a new language, it executes on a Java virtual machine, reusing that technology. This paper explores Scala as language for future real-time systems....

  20. Real-Time Sensor-Actuator Networks

    OpenAIRE

    Sastry, Shivakumar; S. S. Iyengar

    2005-01-01

    Emerging technologies offer new paradigms for computation, control, collaboration, and communication. To realize the full potential of these technologies in industry, defense, and homeland security applications, it is necessary to exploit the real-time distributed computing capabilities of sensor-actuator networks. To reliably design and develop such networks, it is necessary to develop deeper insight into the underlying model for real-time computation and the infrastructure at the node level...

  1. Real Time Information Fusion in Military Systems

    Directory of Open Access Journals (Sweden)

    E. Bhagiratharao

    1990-01-01

    Full Text Available With the proliferation of sensors on platforms like battle ships and aircraft, the information to be handled by the battlefield commanders has significantly increased in the recent time. From a deluge of information flowing from sensors, the battlefield commander is required to make situation assessment in real-time and take appropriate action. Recent studies by cognitive scientists have indicated that decision making by individuals as well as a team suffer from several biases. For these two reasons, the battlefield commanders need assistance of real-time information fusion systems to take objective assessment of highly dynamic battle situation in real-time information fusion systems to take objective assessment of a highly dynamic battle situation in real-time. The real-time information fusion systems at a single platform level as well as that applicable for geographically distributed platforms is discussed in detail in this paper. It was concluded that by carrying out these activities at the platform level as well as at 'global' level involving several platforms, the limitations in performance of any sensor due to propagation effects or due to enemy counter measures can be significantly minimised or totally eliminated. At the same time the functional effectiveness of each sensor onboard different platforms, becomes better than when it had to operate autonomously within the real-time information fusion facility. By carrying out global real-time information fusion activity in a theatre of war, all the platforms operating in the area will have the benefit of the best sensor in that area on each aspect of the capability. A few examples of real-time information fusion system are also discussed.

  2. The LAA real-time benchmarks

    Energy Technology Data Exchange (ETDEWEB)

    Block, R.K.; Krischer, W.; Lone, S. [CERN, Geneva (Switzerland)

    1989-04-01

    In the context of the LAA detector development program a subgroup Real Time Data Processing has tackled the problem of intelligent triggering. The main goal of this group is to show how fast digital devices, implemented as custom-made or commercial processors, can execute some basic algorithms, and how they can be embedded in the data flow between detector readout components and fully programmable commercial processors, which are expected to be the final data processing filter in real time.

  3. Analysis of real-time vibration data

    Science.gov (United States)

    Safak, E.

    2005-01-01

    In recent years, a few structures have been instrumented to provide continuous vibration data in real time, recording not only large-amplitude motions generated by extreme loads, but also small-amplitude motions generated by ambient loads. The main objective in continuous recording is to track any changes in structural characteristics, and to detect damage after an extreme event, such as an earthquake or explosion. The Fourier-based spectral analysis methods have been the primary tool to analyze vibration data from structures. In general, such methods do not work well for real-time data, because real-time data are mainly composed of ambient vibrations with very low amplitudes and signal-to-noise ratios. The long duration, linearity, and the stationarity of ambient data, however, allow us to utilize statistical signal processing tools, which can compensate for the adverse effects of low amplitudes and high noise. The analysis of real-time data requires tools and techniques that can be applied in real-time; i.e., data are processed and analyzed while being acquired. This paper presents some of the basic tools and techniques for processing and analyzing real-time vibration data. The topics discussed include utilization of running time windows, tracking mean and mean-square values, filtering, system identification, and damage detection.

  4. Crystal structure of an essential enzyme in seed starch degradation - barley limit dextrinase in complex with cyclodextrins

    DEFF Research Database (Denmark)

    Vester-Christensen, Malene Bech; Abou Hachem, Maher; Svensson, Birte

    2010-01-01

    Barley limit dextrinase [Hordeum vulgare limit dextrinase (HvLD)] catalyzes the hydrolysis of α-1,6 glucosidic linkages in limit dextrins. This activity plays a role in starch degradation during germination and presumably in starch biosynthesis during grain filling. The crystal structures of Hv......LD in complex with the competitive inhibitors α-cyclodextrin (CD) and β-CD are solved and refined to 2.5 Å and 2.1 Å, respectively, and are the first structures of a limit dextrinase. HvLD belongs to glycoside hydrolase 13 family and is composed of four domains: an immunoglobulin-like N-terminal eight......-stranded β-sandwich domain, a six-stranded β-sandwich domain belonging to the carbohydrate binding module 48 family, a catalytic (β/α)8-like barrel domain that lacks α-helix 5, and a C-terminal eight-stranded β-sandwich domain of unknown function. The CDs are bound at the active site occupying carbohydrate...

  5. Protein and hordein fraction content in barley seeds as affected by sowing date and their relations to malting quality

    Institute of Scientific and Technical Information of China (English)

    QI Jun-cong; CHEN Jin-xin; WANG Jun-mei; WU Fei-bo; CAO Lian-pu; ZHANG Guo-ping

    2005-01-01

    The effect of sowing date on grain protein, hordein fraction content and malting quality of two-rowed spring barley was investigated by using ten commercial cultivars with different grain protein content and the relationships among these traits were examined. The results showed that grain protein content and B hordein content increased as the sowing date postponed and were significantly affected by sowing date, while C and D hordein contents were less influenced by sowing date. There were significant differences in grain protein and hordein fraction content among the ten cultivars. The coefficient of variation of D hordein content was much larger than that of B and C hordein contents, suggesting its greater variation caused by different sowing dates. Beta-amylase activity and diastatic power were also significantly affected by sowing date, with malt extract being less affected. Significant differences in measured malt quality were found among the ten cultivars. Grain protein was significantly correlated with B hordein and malt extract positively and negatively, respectively. There was no significant correlation between beta-amylase activity or diastatic power and grain protein content. B hordein was negatively and significantly correlated with malt extract, but no significant correlations between C hordein, D hordein and malting quality traits.

  6. REAL TIME SYSTEM OPERATIONS 2006-2007

    Energy Technology Data Exchange (ETDEWEB)

    Eto, Joseph H.; Parashar, Manu; Lewis, Nancy Jo

    2008-08-15

    The Real Time System Operations (RTSO) 2006-2007 project focused on two parallel technical tasks: (1) Real-Time Applications of Phasors for Monitoring, Alarming and Control; and (2) Real-Time Voltage Security Assessment (RTVSA) Prototype Tool. The overall goal of the phasor applications project was to accelerate adoption and foster greater use of new, more accurate, time-synchronized phasor measurements by conducting research and prototyping applications on California ISO's phasor platform - Real-Time Dynamics Monitoring System (RTDMS) -- that provide previously unavailable information on the dynamic stability of the grid. Feasibility assessment studies were conducted on potential application of this technology for small-signal stability monitoring, validating/improving existing stability nomograms, conducting frequency response analysis, and obtaining real-time sensitivity information on key metrics to assess grid stress. Based on study findings, prototype applications for real-time visualization and alarming, small-signal stability monitoring, measurement based sensitivity analysis and frequency response assessment were developed, factory- and field-tested at the California ISO and at BPA. The goal of the RTVSA project was to provide California ISO with a prototype voltage security assessment tool that runs in real time within California ISO?s new reliability and congestion management system. CERTS conducted a technical assessment of appropriate algorithms, developed a prototype incorporating state-of-art algorithms (such as the continuation power flow, direct method, boundary orbiting method, and hyperplanes) into a framework most suitable for an operations environment. Based on study findings, a functional specification was prepared, which the California ISO has since used to procure a production-quality tool that is now a part of a suite of advanced computational tools that is used by California ISO for reliability and congestion management.

  7. Visualization of Real-Time Data

    Science.gov (United States)

    Stansifer, Ryan; Engrand, Peter

    1996-01-01

    In this project we explored various approaches to presenting real-time data from the numerous systems monitored on the space shuttle to computer users. We examined the approach that several projects at the Kennedy Space Center (KSC) used to accomplish this. We undertook to build a prototype system to demonstrate that the Internet and the Java programming language could be used to present the real-time data conveniently. Several Java programs were developed that presented real-time data in different forms including one form that emulated the display screens of the PC GOAL system which is familiar to many at KSC. Also, we developed several communications programs to supply the data continuously. Furthermore, a framework was created using the World Wide Web (WWW) to organize the collection and presentation of the real-time data. We believe our demonstration project shows the great flexibility of the approach. We had no particular use of the data in mind, instead we wanted the most general and the least complex framework possible. People who wish to view data need only know how to use a WWW browser and the address (the URL). People wanting to build WWW documents containing real-time data need only know the values of a few parameters, they do not need to program in Java or any other language. These are stunning advantages over more monolithic systems.

  8. Differential labelling of cysteines for simultaneous identification of thioredoxin h-reducible disulphides in native protein extracts: insight into recognition and regulation of proteins in barley seeds by thioredoxin h

    DEFF Research Database (Denmark)

    Maeda, Kenji; Finnie, Christine; Svensson, Birte

    2005-01-01

    . Mass shifts of 15 peptides, induced by treatment with thioredoxin h and differential alkylation, identified specific reduction of nine disulphides in BASI, four a-amylase/trypsin inhibitors and a protein of unknown function. Two specific disulphides, located structurally close to the alpha......-amylase/subtilisin inhibitor (BASI) by barley thioredoxin h isoform 1 was analysed. Furthermore, the method was coupled with twodimensional electrophoresis for convenient thioredoxin h-reducible disulphide identification in barley seed extracts without the need for protein purification or production of recombinant proteins......-amylase binding surfaces of BASI and alpha-amylase inhibitor BMAI-1 were demonstrated to be reduced to a particularly high extent. For the first time, specificity of thioredoxin h for particular disulphide bonds is demonstrated, providing a basis to study structural aspects of the recognition mechanism...

  9. Deterministic Real-time Thread Scheduling

    CERN Document Server

    Yun, Heechul; Sha, Lui

    2011-01-01

    Race condition is a timing sensitive problem. A significant source of timing variation comes from nondeterministic hardware interactions such as cache misses. While data race detectors and model checkers can check races, the enormous state space of complex software makes it difficult to identify all of the races and those residual implementation errors still remain a big challenge. In this paper, we propose deterministic real-time scheduling methods to address scheduling nondeterminism in uniprocessor systems. The main idea is to use timing insensitive deterministic events, e.g, an instruction counter, in conjunction with a real-time clock to schedule threads. By introducing the concept of Worst Case Executable Instructions (WCEI), we guarantee both determinism and real-time performance.

  10. Continuous, real time microwave plasma element sensor

    Science.gov (United States)

    Woskov, Paul P.; Smatlak, Donna L.; Cohn, Daniel R.; Wittle, J. Kenneth; Titus, Charles H.; Surma, Jeffrey E.

    1995-01-01

    Microwave-induced plasma for continuous, real time trace element monitoring under harsh and variable conditions. The sensor includes a source of high power microwave energy and a shorted waveguide made of a microwave conductive, refractory material communicating with the source of the microwave energy to generate a plasma. The high power waveguide is constructed to be robust in a hot, hostile environment. It includes an aperture for the passage of gases to be analyzed and a spectrometer is connected to receive light from the plasma. Provision is made for real time in situ calibration. The spectrometer disperses the light, which is then analyzed by a computer. The sensor is capable of making continuous, real time quantitative measurements of desired elements, such as the heavy metals lead and mercury.

  11. Durham adaptive optics real-time controller.

    Science.gov (United States)

    Basden, Alastair; Geng, Deli; Myers, Richard; Younger, Eddy

    2010-11-10

    The Durham adaptive optics (AO) real-time controller was initially a proof of concept design for a generic AO control system. It has since been developed into a modern and powerful central-processing-unit-based real-time control system, capable of using hardware acceleration (including field programmable gate arrays and graphical processing units), based primarily around commercial off-the-shelf hardware. It is powerful enough to be used as the real-time controller for all currently planned 8 m class telescope AO systems. Here we give details of this controller and the concepts behind it, and report on performance, including latency and jitter, which is less than 10 μs for small AO systems.

  12. Interactive Real-time Magnetic Resonance Imaging

    DEFF Research Database (Denmark)

    Brix, Lau

    seeks to implement and assess existing reconstruction algorithms using multi-processors of modern graphics cards and many-core computer processors and to cover some of the potential clinical applications which might benefit from using an interactive real-time MRI system. First an off......-line, but interactive, slice alignment tool was used to support the notion that 3D blood flow quantification in the heart possesses the ability to obtain curves and volumes which are not statistical different from standard 2D flow. Secondly, the feasibility of an interactive real-time MRI system was exploited...... with regard to optimal sampling strategy for detecting motion in four different anatomies on two different MRI scanner brands. A fully implemented interactive real-time MRI system was exploited in a group of healthy fetuses and proved its eligibility as an alternative diagnostic tool for fetal imaging...

  13. Real-Time Visualization of Tissue Ischemia

    Science.gov (United States)

    Bearman, Gregory H. (Inventor); Chrien, Thomas D. (Inventor); Eastwood, Michael L. (Inventor)

    2000-01-01

    A real-time display of tissue ischemia which comprises three CCD video cameras, each with a narrow bandwidth filter at the correct wavelength is discussed. The cameras simultaneously view an area of tissue suspected of having ischemic areas through beamsplitters. The output from each camera is adjusted to give the correct signal intensity for combining with, the others into an image for display. If necessary a digital signal processor (DSP) can implement algorithms for image enhancement prior to display. Current DSP engines are fast enough to give real-time display. Measurement at three, wavelengths, combined into a real-time Red-Green-Blue (RGB) video display with a digital signal processing (DSP) board to implement image algorithms, provides direct visualization of ischemic areas.

  14. Low Phytic Acid Barley Responses to Phosphorus Rates

    Science.gov (United States)

    Low phytic acid (LPA) barley (Hordeum vulgare L.) cultivars partition phosphorus in seed tissue differently than conventional barley cultivars through a reduction in seed phytic acid (myo-inositol-1,2,3,4,5,6-hexkisphosphate) coupled with an increase in inorganic phosphorus. The response of the LPA...

  15. Real Time Implementation Of Face Recognition System

    Directory of Open Access Journals (Sweden)

    Megha Manchanda

    2014-10-01

    Full Text Available This paper proposes face recognition method using PCA for real time implementation. Nowadays security is gaining importance as it is becoming necessary for people to keep passwords in their mind and carry cards. Such implementations however, are becoming less secure and practical, also is becoming more problematic thus leading to an increasing interest in techniques related to biometrics systems. Face recognition system is amongst important subjects in biometrics systems. This system is very useful for security in particular and has been widely used and developed in many countries. This study aims to achieve face recognition successfully by detecting human face in real time, based on Principal Component Analysis (PCA algorithm.

  16. Real-time systems scheduling fundamentals

    CERN Document Server

    Chetto, Maryline

    2014-01-01

    Real-time systems are used in a wide range of applications, including control, sensing, multimedia, etc.  Scheduling is a central problem for these computing/communication systems since responsible of software execution in a timely manner. This book provides state of knowledge in this domain with special emphasis on the key results obtained within the last decade. This book addresses foundations as well as the latest advances and findings in Real-Time Scheduling, giving all references to important papers. But nevertheless the chapters will be short and not overloaded with confusing details.

  17. Machine vision for real time orbital operations

    Science.gov (United States)

    Vinz, Frank L.

    1988-01-01

    Machine vision for automation and robotic operation of Space Station era systems has the potential for increasing the efficiency of orbital servicing, repair, assembly and docking tasks. A machine vision research project is described in which a TV camera is used for inputing visual data to a computer so that image processing may be achieved for real time control of these orbital operations. A technique has resulted from this research which reduces computer memory requirements and greatly increases typical computational speed such that it has the potential for development into a real time orbital machine vision system. This technique is called AI BOSS (Analysis of Images by Box Scan and Syntax).

  18. Axial Tomography from Digitized Real Time Radiography

    Science.gov (United States)

    Zolnay, A. S.; McDonald, W. M.; Doupont, P. A.; McKinney, R. L.; Lee, M. M.

    1985-01-18

    Axial tomography from digitized real time radiographs provides a useful tool for industrial radiography and tomography. The components of this system are: x-ray source, image intensifier, video camera, video line extractor and digitizer, data storage and reconstruction computers. With this system it is possible to view a two dimensional x-ray image in real time at each angle of rotation and select the tomography plane of interest by choosing which video line to digitize. The digitization of a video line requires less than a second making data acquisition relatively short. Further improvements on this system are planned and initial results are reported.

  19. Real-time systems scheduling 2 focuses

    CERN Document Server

    Chetto, Maryline

    2014-01-01

    Real-time systems are used in a wide range of applications, including control, sensing, multimedia, etc. Scheduling is a central problem for these computing/communication systems since it is responsible for software execution in a timely manner. This book, the second of two volumes on the subject, brings together knowledge on specific topics and discusses the recent advances for some of them.  It addresses foundations as well as the latest advances and findings in real-time scheduling, giving comprehensive references to important papers, but the chapters are short and not overloaded with co

  20. Real time estimates of GDP growth

    NARCIS (Netherlands)

    Groot, de E.A. (Bert); Franses, P.H.P.H.

    2005-01-01

    This paper describes the components of the EICIE, the Econometric Institute Current Indicator of the Economy. This measure concerns quarterly and annual growth of Dutch real Gross Domestic Product. The key component of our real-time forecasting model for Dutch quarterly GDP is weekly staffing servic

  1. Real time estimates of GDP growth

    NARCIS (Netherlands)

    E.A. de Groot (Bert); Ph.H.B.F. Franses (Philip Hans)

    2005-01-01

    textabstractThis paper describes the components of the EICIE, the Econometric Institute Current Indicator of the Economy. This measure concerns quarterly and annual growth of Dutch real Gross Domestic Product. The key component of our real-time forecasting model for Dutch quarterly GDP is weekly sta

  2. Advances in Real-Time Systems

    CERN Document Server

    Chakraborty, Samarjit

    2012-01-01

    This volume contains the lectures given in honor to Georg Farber as tribute to his contributions in the area of real-time and embedded systems. The chapters of many leading scientists cover a wide range of aspects, like robot or automotive vision systems or medical aspects.

  3. Scene independent real-time indirect illumination

    DEFF Research Database (Denmark)

    Frisvad, Jeppe Revall; Christensen, Niels Jørgen; Falster, Peter

    2005-01-01

    A novel method for real-time simulation of indirect illumination is presented in this paper. The method, which we call Direct Radiance Mapping (DRM), is based on basal radiance calculations and does not impose any restrictions on scene geometry or dynamics. This makes the method tractable for real...

  4. Real Time Grid Reliability Management 2005

    Energy Technology Data Exchange (ETDEWEB)

    Eto, Joe; Eto, Joe; Lesieutre, Bernard; Lewis, Nancy Jo; Parashar, Manu

    2008-07-07

    The increased need to manage California?s electricity grid in real time is a result of the ongoing transition from a system operated by vertically-integrated utilities serving native loads to one operated by an independent system operator supporting competitive energy markets. During this transition period, the traditional approach to reliability management -- construction of new transmission lines -- has not been pursued due to unresolved issues related to the financing and recovery of transmission project costs. In the absence of investments in new transmission infrastructure, the best strategy for managing reliability is to equip system operators with better real-time information about actual operating margins so that they can better understand and manage the risk of operating closer to the edge. A companion strategy is to address known deficiencies in offline modeling tools that are needed to ground the use of improved real-time tools. This project: (1) developed and conducted first-ever demonstrations of two prototype real-time software tools for voltage security assessment and phasor monitoring; and (2) prepared a scoping study on improving load and generator response models. Additional funding through two separate subsequent work authorizations has already been provided to build upon the work initiated in this project.

  5. Refactoring Real-Time Java Profiles

    DEFF Research Database (Denmark)

    Søndergaard, Hans; Thomsen, Bent; Ravn, Anders Peter

    2011-01-01

    Just like other software, Java profiles benefits from refactoring when they have been used and have evolved for some time. This paper presents a refactoring of the Real-Time Specification for Java (RTSJ) and the Safety Critical Java (SCJ) profile (JSR-302). It highlights core concepts and makes...

  6. Real-time analysis of telemetry data

    Science.gov (United States)

    Kao, Simon A.; Laffey, Thomas J.; Schmidt, James L.; Read, Jackson Y.; Dunham, Larry L.

    1987-01-01

    This paper descibes a knowledge-based system for performing real-time monitoring and analysis of telemetry data from the NASA Hubble Space Telescope (HST). In order to handle asynchronous inputs and perform in real time the system consists of three or more separate processes, which run concurrently and communicate via a message passing scheme. The data management process gathers, compresses, and scales the incoming telemetry data befoe sending it to the other tasks. The inferencing process uses the incoming data to perform a real-time analysis of the state and health of the Space Telescope. The I/O process receives telemetry monitors from the data management process, updates its graphical displays in real time, and acts as the interface to the console operator. The three processes may run on the same or different computers. This system is currently under development and is being used to monitor testcases produced by the Bass Telemetry System in the Hardware/Software Integration Facility at Lockheed Missile and Space Co. in Sunnyvale, California.

  7. Real-time dynamics of proton decay

    CERN Document Server

    Grigoriev, D

    2005-01-01

    Substituting Skyrmion for nucleon, one can potentially see -- in real time -- how the monopole is catalysing the proton (or neutron) decay, and even obtain a plausible estimate for catalysis cross-section. Here we discuss the key aspects of a practical implementation of such approach and demonstrate how one can overcome the main technical problems: Gauss constraint violation and reflections at the boundaries.

  8. The Power of Real-Time PCR

    Science.gov (United States)

    Valasek, Mark A.; Repa, Joyce J.

    2005-01-01

    In recent years, real-time polymerase chain reaction (PCR) has emerged as a robust and widely used methodology for biological investigation because it can detect and quantify very small amounts of specific nucleic acid sequences. As a research tool, a major application of this technology is the rapid and accurate assessment of changes in gene…

  9. Feedback as Real-Time Constructions

    Science.gov (United States)

    Keiding, Tina Bering; Qvortrup, Ane

    2014-01-01

    This article offers a re-description of feedback and the significance of time in feedback constructions based on systems theory. It describes feedback as internal, real-time constructions in a learning system. From this perspective, feedback is neither immediate nor delayed, but occurs in the very moment it takes place. This article argues for a…

  10. Real Time Structured Light and Applications

    DEFF Research Database (Denmark)

    Wilm, Jakob

    , increased processing power, and methods presented in this thesis, it is possible to perform structured light scans in real time with 20 depth measurements per second. This offers new opportunities for studying dynamic scenes, quality control, human-computer interaction and more. This thesis discusses...

  11. Real-time Texture Error Detection

    Directory of Open Access Journals (Sweden)

    Dan Laurentiu Lacrama

    2008-01-01

    Full Text Available This paper advocates an improved solution for the real-time error detection of texture errors that occurs in the production process in textile industry. The research is focused on the mono-color products with 3D texture model (Jacquard fabrics. This is a more difficult task than, for example, 2D multicolor textures.

  12. Using Real-Time PCR as a tool for monitoring the authenticity of commercial coffees.

    Science.gov (United States)

    Ferreira, Thiago; Farah, Adriana; Oliveira, Tatiane C; Lima, Ivanilda S; Vitório, Felipe; Oliveira, Edna M M

    2016-05-15

    Coffee is one of the main food products commercialized in the world. Its considerable market value among food products makes it susceptible to adulteration, especially with cereals. Therefore, the objective of this study was to develop a method based on Real-Time Polymerase Chain Reaction (PCR) for detection of cereals in commercial ground roast and soluble coffees. After comparison with standard curves obtained by serial dilution of DNA extracted from barley, corn and rice, the method was sensitive and specific to quantify down to 0.6 pg, 14 pg and 16 pg of barley, corn and rice DNA, respectively. To verify the applicability of the method, 30 commercial samples obtained in different countries were evaluated and those classified as gourmets or superior did not present the tested cereals DNA. However, barley was detected in various traditional (cheaper) samples from South America. In addition, corn and rice were also detected in different samples. Real-Time PCR showed to be suitable for detection of food adulterants in commercial ground roast and soluble coffees.

  13. Collecting data in real time with postcards

    DEFF Research Database (Denmark)

    2013-01-01

    The success of information technology (IT) in transforming healthcare is often limited by the lack of clear understanding of the context at which the technology is used. Various methods have been proposed to understand healthcare context better in designing and implementing Health Information...... Systems. These methods often involve cross-sectional, retrospective data collection. This paper describes the postcard method for prospective real-time data collection, both in paper format and electronic format. This paper then describes the results obtained using postcard techniques in Denmark...... and Australia. The benefits of this technique are illustrated. There are limitations in using postcard techniques and this paper provides a detail discussion about these limitations. Postcard techniques provide unique advantages in understanding real time healthcare context and it is an important technique...

  14. Real-Time Watercolor for Animation

    Institute of Scientific and Technical Information of China (English)

    Thomas Luft; Oliver Deussen

    2006-01-01

    We present algorithms that allow for real-time rendering of 3D-scenes with a watercolor painting appearance. Our approach provides an appropriate simplification of the visual complexity, imitates characteristic natural effects of watercolor, and provides two essential painting techniques: the wet-on-wet and the wet-on-dry painting. We concentrate on efficient algorithms based on image space processing rather than on an exact simulation. This allows for the real-time rendering of 3D-scenes. During an animation a high frame-to-frame coherence can be achieved due to a stable segmentation scheme. Finally, we seamlessly integrate a smooth illumination into the watercolor renderings using information from the 3D-scene.

  15. Real Time Radiation Exposure And Health Risks

    Science.gov (United States)

    Hu, Shaowen; Barzilla, Janet E.; Semones, Edward J.

    2015-01-01

    Radiation from solar particle events (SPEs) poses a serious threat to future manned missions outside of low Earth orbit (LEO). Accurate characterization of the radiation environment in the inner heliosphere and timely monitoring the health risks to crew are essential steps to ensure the safety of future Mars missions. In this project we plan to develop an approach that can use the particle data from multiple satellites and perform near real-time simulations of radiation exposure and health risks for various exposure scenarios. Time-course profiles of dose rates will be calculated with HZETRN and PDOSE from the energy spectrum and compositions of the particles archived from satellites, and will be validated from recent radiation exposure measurements in space. Real-time estimation of radiation risks will be investigated using ARRBOD. This cross discipline integrated approach can improve risk mitigation by providing critical information for risk assessment and medical guidance to crew during SPEs.

  16. Monte Carlo study of real time dynamics

    CERN Document Server

    Alexandru, Andrei; Bedaque, Paulo F; Vartak, Sohan; Warrington, Neill C

    2016-01-01

    Monte Carlo studies involving real time dynamics are severely restricted by the sign problem that emerges from highly oscillatory phase of the path integral. In this letter, we present a new method to compute real time quantities on the lattice using the Schwinger-Keldysh formalism via Monte Carlo simulations. The key idea is to deform the path integration domain to a complex manifold where the phase oscillations are mild and the sign problem is manageable. We use the previously introduced "contraction algorithm" to create a Markov chain on this alternative manifold. We substantiate our approach by analyzing the quantum mechanical anharmonic oscillator. Our results are in agreement with the exact ones obtained by diagonalization of the Hamiltonian. The method we introduce is generic and in principle applicable to quantum field theory albeit very slow. We discuss some possible improvements that should speed up the algorithm.

  17. Real time PCR. Application in dengue studies

    Directory of Open Access Journals (Sweden)

    Jeanette Prada-Arismendy

    2011-06-01

    Full Text Available PCR (polymerase chain reaction is a routinely used tool in every diagnostic and research laboratory. This technique has been used in detection of mutations and pathogens, forensic investigation, and even is the base tool for human genome sequencing. A modification of PCR technique, real time PCR, allows the quantification of nucleic acids with higher sensibility, specificity and reproducibility. This article is intended to clarify the foundations of real-time PCR, using an application model for virology. In the actual work, it was quantified the viral load of dengue virus serotype 2 produced from infected murine macrophages; the obtained results in this work established that murine strain BALB/c presents a greater susceptibility to dengue virus infection, which establishes BALB/c murine strain as a best model of study for investigation of dengue virus infection physiopathology.

  18. AMON: Transition to real-time operations

    Science.gov (United States)

    Cowen, D. F.; Keivani, A.; Tešić, G.

    2016-04-01

    The Astrophysical Multimessenger Observatory Network (AMON) will link the world's leading high-energy neutrino, cosmic-ray, gamma-ray and gravitational wave observatories by performing real-time coincidence searches for multimessenger sources from observatories' subthreshold data streams. The resulting coincidences will be distributed to interested parties in the form of electronic alerts for real-time follow-up observation. We will present the science case, design elements, current and projected partner observatories, status of the AMON project, and an initial AMON-enabled analysis. The prototype of the AMON server has been online since August 2014 and processing archival data. Currently, we are deploying new high-uptime servers and will be ready to start issuing alerts as early as winter 2015/16.

  19. System Equivalent for Real Time Digital Simulator

    Science.gov (United States)

    Lin, Xi

    2011-07-01

    The purpose of this research is to develop a method of making system equivalents for the Real Time Digital Simulator (RTDS), which should enhance its capability of simulating large power systems. The proposed equivalent combines a Frequency Dependent Network Equivalent (FDNE) for the high frequency electromagnetic transients and a Transient Stability Analysis (TSA) type simulation block for the electromechanical transients. The frequency dependent characteristic for FDNE is obtained by curve-fitting frequency domain admittance characteristics using the Vector Fitting method. An approach for approximating the frequency dependent characteristic of large power networks from readily available typical power-flow data is also introduced. A new scheme of incorporating TSA solution in RTDS is proposed. This report shows how the TSA algorithm can be adapted to a real time platform. The validity of this method is confirmed with examples, including the study of a multi in-feed HVDC system based network.

  20. Real time gamma-ray signature identifier

    Science.gov (United States)

    Rowland, Mark [Alamo, CA; Gosnell, Tom B [Moraga, CA; Ham, Cheryl [Livermore, CA; Perkins, Dwight [Livermore, CA; Wong, James [Dublin, CA

    2012-05-15

    A real time gamma-ray signature/source identification method and system using principal components analysis (PCA) for transforming and substantially reducing one or more comprehensive spectral libraries of nuclear materials types and configurations into a corresponding concise representation/signature(s) representing and indexing each individual predetermined spectrum in principal component (PC) space, wherein an unknown gamma-ray signature may be compared against the representative signature to find a match or at least characterize the unknown signature from among all the entries in the library with a single regression or simple projection into the PC space, so as to substantially reduce processing time and computing resources and enable real-time characterization and/or identification.

  1. Real Time Radiation Monitoring Using Nanotechnology

    Science.gov (United States)

    Li, Jing (Inventor); Wilkins, Richard T. (Inventor); Hanratty, James J. (Inventor); Lu, Yijiang (Inventor)

    2016-01-01

    System and method for monitoring receipt and estimating flux value, in real time, of incident radiation, using two or more nanostructures (NSs) and associated terminals to provide closed electrical paths and to measure one or more electrical property change values .DELTA.EPV, associated with irradiated NSs, during a sequence of irradiation time intervals. Effects of irradiation, without healing and with healing, of the NSs, are separately modeled for first order and second order healing. Change values.DELTA.EPV are related to flux, to cumulative dose received by NSs, and to radiation and healing effectivity parameters and/or.mu., associated with the NS material and to the flux. Flux and/or dose are estimated in real time, based on EPV change values, using measured .DELTA.EPV values. Threshold dose for specified changes of biological origin (usually undesired) can be estimated. Effects of time-dependent radiation flux are analyzed in pre-healing and healing regimes.

  2. "Fast" Is Not "Real-Time": Designing Effective Real-Time AI Systems

    Science.gov (United States)

    O'Reilly, Cindy A.; Cromarty, Andrew S.

    1985-04-01

    Realistic practical problem domains (such as robotics, process control, and certain kinds of signal processing) stand to benefit greatly from the application of artificial intelligence techniques. These problem domains are of special interest because they are typified by complex dynamic environments in which the ability to select and initiate a proper response to environmental events in real time is a strict prerequisite to effective environmental interaction. Artificial intelligence systems developed to date have been sheltered from this real-time requirement, however, largely by virtue of their use of simplified problem domains or problem representations. The plethora of colloquial and (in general) mutually inconsistent interpretations of the term "real-time" employed by workers in each of these domains further exacerbates the difficul-ties in effectively applying state-of-the-art problem solving tech-niques to time-critical problems. Indeed, the intellectual waters are by now sufficiently muddied that the pursuit of a rigorous treatment of intelligent real-time performance mandates the redevelopment of proper problem perspective on what "real-time" means, starting from first principles. We present a simple but nonetheless formal definition of real-time performance. We then undertake an analysis of both conventional techniques and AI technology with respect to their ability to meet substantive real-time performance criteria. This analysis provides a basis for specification of problem-independent design requirements for systems that would claim real-time performance. Finally, we discuss the application of these design principles to a pragmatic problem in real-time signal understanding.

  3. Real-time RGBD SLAM system

    Science.gov (United States)

    Czupryński, BłaŻej; Strupczewski, Adam

    2015-09-01

    A real-time tracking and mapping SLAM system is presented. The developed system uses input from an RGBD sensor and tracks the camera pose from frame to frame. The tracking is based on matched feature points and is performed with respect to selected keyframes. The system is robust and scalable, as an arbitrary number of keyframes can be chosen for visualization and tracking depending on the desired accuracy and speed. The presented system is also a good platform for further research.

  4. Tactical AI in Real Time Strategy Games

    Science.gov (United States)

    2015-03-26

    a parent , and each parent can have multiple children based on what decision options are available at the parent node. The search evaluates each child...The real time strategy (RTS) tactical decision making problem is a difficult problem. It is generally more complex due to its high degree of time...evolutionary algorithms (MOEAs) in this tactical decision making problem allows an AI agent to make fast, effective solutions that do not require modification

  5. Robust synthesis for real-time systems

    DEFF Research Database (Denmark)

    Larsen, Kim Guldstrand; Legay, Axel; Traonouez, Louis-Marie;

    2014-01-01

    Specification theories for real-time systems allow reasoning about interfaces and their implementation models, using a set of operators that includes satisfaction, refinement, logical and parallel composition. To make such theories applicable throughout the entire design process from an abstract ...... strategies in timed games. Finally, we consider the parametric robustness problem and propose a counter-example refinement heuristic for computing safe perturbation values....

  6. Real-time optical information processing

    CERN Document Server

    Javidi, Bahram

    1994-01-01

    Real-Time Optical Information Processing covers the most recent developments in optical information processing, pattern recognition, neural computing, and materials for devices in optical computing. Intended for researchers and graduate students in signal and information processing with some elementary background in optics, the book provides both theoretical and practical information on the latest in information processing in all its aspects. Leading researchers in the field describe the significant signal processing algorithms architectures in optics as well as basic hardware concepts,

  7. Real-Time Neutron Radiography at CARR

    Institute of Scientific and Technical Information of China (English)

    HE; Lin-feng; HAN; Song-bai; WANG; Hong-li; WU; Mei-mei; WEI; Guo-hai; WANG; Yu

    2012-01-01

    <正>A real-time detector system for neutron radiography based on CMOS camera has been designed for the thermal neutron imaging facility under construction at China Advanced Research Reactor (CARR). This system is equipped with a new scientific CMOS camera with 5.5 million pixels and speed up to 100 fps at full frame. The readout noise is less than 2.4 electron per pixel. It is capable of providing

  8. Real Time Route for Dynamic Road Congestions

    Directory of Open Access Journals (Sweden)

    A. M. Riad

    2012-05-01

    Full Text Available Minimizing service delivery and travel time during rush hours downtown is strategic target for several organizations, especially the emergency organizations. This paper presents an On-line and Real-time Dynamic Route System (ORDRS which benefits from the advantages and integration between information system and communications technology. It utilizes Global Positioning System (GPS, Geographical Information Systems (GIS, and Global System for Mobile communications (GSM; for producing the real time routes for vehicles. GPS-Tracker is the main input device for ORDRS. It is fixated in a vehicle, sends vehicle's movement data (Geo-info to the control center wirelessly through either Short Message Service (SMS or General Packet Radio Service (GPRS. Geo-info includes time, date, longitude, latitude, speed, and etc., these data is classified over time during weekdays into interval time slices, each slice is 30 minutes. Speeds are treated by GIS tools to determine historical and real time speeds for each street segment in the road network which is being used for calculating time impedance (cost matrix for each street segment dynamically. ORDRS uses a cost matrix of the current time slice for determining the best route to each vehicle in duty attached. Several algorithms was used to calculate the shortest route, a comparison between Dijekstra and Yen algorithms was studied.

  9. Real-time Interactive Tree Animation.

    Science.gov (United States)

    Quigley, Ed; Yu, Yue; Huang, Jingwei; Lin, Winnie; Fedkiw, Ronald

    2017-01-30

    We present a novel method for posing and animating botanical tree models interactively in real time. Unlike other state of the art methods which tend to produce trees that are overly flexible, bending and deforming as if they were underwater plants, our approach allows for arbitrarily high stiffness while still maintaining real-time frame rates without spurious artifacts, even on quite large trees with over ten thousand branches. This is accomplished by using an articulated rigid body model with as-stiff-as-desired rotational springs in conjunction with our newly proposed simulation technique, which is motivated both by position based dynamics and the typical O(N) algorithms for articulated rigid bodies. The efficiency of our algorithm allows us to pose and animate trees with millions of branches or alternatively simulate a small forest comprised of many highly detailed trees. Even using only a single CPU core, we can simulate ten thousand branches in real time while still maintaining quite crisp user interactivity. This has allowed us to incorporate our framework into a commodity game engine to run interactively even on a low-budget tablet. We show that our method is amenable to the incorporation of a large variety of desirable effects such as wind, leaves, fictitious forces, collisions, fracture, etc.

  10. Barley germination

    DEFF Research Database (Denmark)

    Daneri-Castro, Sergio N.; Svensson, Birte; Roberts, Thomas H.

    2016-01-01

    conditions continue to be key to discovering the roles of individual protein forms and posttranslational modifications, such as glycosylation. Activity-based proteomics, particularly in combination with new gene editing technologies, has great potential to elucidate the network of enzymes in barley...

  11. Exploring Earthquakes in Real-Time

    Science.gov (United States)

    Bravo, T. K.; Kafka, A. L.; Coleman, B.; Taber, J. J.

    2013-12-01

    Earthquakes capture the attention of students and inspire them to explore the Earth. Adding the ability to view and explore recordings of significant and newsworthy earthquakes in real-time makes the subject even more compelling. To address this opportunity, the Incorporated Research Institutions for Seismology (IRIS), in collaboration with Moravian College, developed ';jAmaSeis', a cross-platform application that enables students to access real-time earthquake waveform data. Students can watch as the seismic waves are recorded on their computer, and can be among the first to analyze the data from an earthquake. jAmaSeis facilitates student centered investigations of seismological concepts using either a low-cost educational seismograph or streamed data from other educational seismographs or from any seismic station that sends data to the IRIS Data Management System. After an earthquake, students can analyze the seismograms to determine characteristics of earthquakes such as time of occurrence, distance from the epicenter to the station, magnitude, and location. The software has been designed to provide graphical clues to guide students in the analysis and assist in their interpretations. Since jAmaSeis can simultaneously record up to three stations from anywhere on the planet, there are numerous opportunities for student driven investigations. For example, students can explore differences in the seismograms from different distances from an earthquake and compare waveforms from different azimuthal directions. Students can simultaneously monitor seismicity at a tectonic plate boundary and in the middle of the plate regardless of their school location. This can help students discover for themselves the ideas underlying seismic wave propagation, regional earthquake hazards, magnitude-frequency relationships, and the details of plate tectonics. The real-time nature of the data keeps the investigations dynamic, and offers students countless opportunities to explore.

  12. CUDA-based real time surgery simulation.

    Science.gov (United States)

    Liu, Youquan; De, Suvranu

    2008-01-01

    In this paper we present a general software platform that enables real time surgery simulation on the newly available compute unified device architecture (CUDA)from NVIDIA. CUDA-enabled GPUs harness the power of 128 processors which allow data parallel computations. Compared to the previous GPGPU, it is significantly more flexible with a C language interface. We report implementation of both collision detection and consequent deformation computation algorithms. Our test results indicate that the CUDA enables a twenty times speedup for collision detection and about fifteen times speedup for deformation computation on an Intel Core 2 Quad 2.66 GHz machine with GeForce 8800 GTX.

  13. Real-time inclinometer using accelerometer MEMS

    CERN Document Server

    Hanto, D; Hermanto, B; Puranto, P; Handoko, L T

    2011-01-01

    A preliminary design of inclinometer for real-time monitoring system of soil displacement is proposed. The system is developed using accelerometer sensor with microelectromechanical system (MEMS) device. The main apparatus consists of a single MEMS sensor attached to a solid pipe and stucked pependicularly far away below the soil surface. The system utilizes small fractions of electrical signals from MEMS sensor induced by the pipe inclination due to soil displacements below the surface. It is argued that the system is accurate enough to detect soil displacements responsible for landslides, and then realizes a simple and low cost landslide early warning system.

  14. Testing Real-Time Systems Using UPPAAL

    DEFF Research Database (Denmark)

    Hessel, Anders; Larsen, Kim Guldstrand; Mikucionis, Marius

    2008-01-01

    This chapter presents principles and techniques for model-based black-box conformance testing of real-time systems using the Uppaal model-checking tool-suite. The basis for testing is given as a network of concurrent timed automata specified by the test engineer. Relativized input....../output conformance serves as the notion of implementation correctness, essentially timed trace inclusion taking environment assumptions into account. Test cases can be generated offline and later executed, or they can be generated and executed online. For both approaches this chapter discusses how to specify test...... objectives, derive test sequences, apply these to the system under test, and assign a verdict....

  15. Real-time detection of gravitational microlensing

    CERN Document Server

    Pratt, M R; Axelrod, T S; Becker, A; Bennett, D P; Cook, K H; Freeman, K C; Griest, K; Guern, J A; Lehner, M; Marshall, S L; Peterson, B A; Quinn, P J; Reiss, D; Rodgers, A W; Stubbs, C W; Sutherland, W; Welch, D

    1995-01-01

    Real-time detection of microlensing has moved from proof of concept in 1994 to a steady stream of events this year. Global dissemination of these events by the MACHO and OGLE collaborations has made possible intensive photometric and spectroscopic followup from widely dispersed sites confirming the microlensing hypothesis. Improved photometry and increased temporal resolution from followup observations greatly increases the possibility of detecting deviations from the standard point-source, point-lens, inertial motion microlensing model. These deviations are crucial in understanding individual lensing systems by breaking the degeneracy between lens mass, position and velocity. We report here on GMAN (Global Microlensing Alert Network), the coordinated followup of MACHO alerts.

  16. Low cost real time interactive analysis system

    Science.gov (United States)

    Stetina, F.

    1988-01-01

    Efforts continue to develop a low cost real time interactive analysis system for the reception of satellite data. A multi-purpose ingest hardware software frame formatter was demonstrated for GOES and TIROS data and work is proceeding on extending the capability to receive GMS data. A similar system was proposed as an archival and analysis system for use with INSAT data and studies are underway to modify the system to receive the planned SeaWiFS (ocean color) data. This system was proposed as the core of a number of international programs in support of U.S. AID activities. Systems delivered or nearing final testing are listed.

  17. Linear Regression Based Real-Time Filtering

    Directory of Open Access Journals (Sweden)

    Misel Batmend

    2013-01-01

    Full Text Available This paper introduces real time filtering method based on linear least squares fitted line. Method can be used in case that a filtered signal is linear. This constraint narrows a band of potential applications. Advantage over Kalman filter is that it is computationally less expensive. The paper further deals with application of introduced method on filtering data used to evaluate a position of engraved material with respect to engraving machine. The filter was implemented to the CNC engraving machine control system. Experiments showing its performance are included.

  18. Real-time image and video processing

    CERN Document Server

    Kehtarnavaz, Nasser

    2006-01-01

    This book presents an overview of the guidelines and strategies for transitioning an image or video processing algorithm from a research environment into a real-time constrained environment. Such guidelines and strategies are scattered in the literature of various disciplines including image processing, computer engineering, and software engineering, and thus have not previously appeared in one place. By bringing these strategies into one place, the book is intended to serve the greater community of researchers, practicing engineers, industrial professionals, who are interested in taking an im

  19. Object detection in real-time

    Science.gov (United States)

    Solder, Ulrich; Graefe, Volker

    1991-03-01

    An algorithm working on monocular gray-scale image sequences for object detection combined with a road tracker is presented. This algorithm appropriate for the real-time demands of an autonomous car driving with speeds over 40 km/h may be used for triggering obstacle avoidance maneuvers such as coming to a safe stop automatically in front of an obstacle or following another car. Moving and static objects have been detected in real-world experiments on various types of roads even under unfavorable weather conditions. . Morgenthaler and

  20. Systems Analyze Water Quality in Real Time

    Science.gov (United States)

    2010-01-01

    A water analyzer developed under Small Business Innovation Research (SBIR) contracts with Kennedy Space Center now monitors treatment processes at water and wastewater facilities around the world. Originally designed to provide real-time detection of nutrient levels in hydroponic solutions for growing plants in space, the ChemScan analyzer, produced by ASA Analytics Inc., of Waukesha, Wisconsin, utilizes spectrometry and chemometric algorithms to automatically analyze multiple parameters in the water treatment process with little need for maintenance, calibration, or operator intervention. The company has experienced a compound annual growth rate of 40 percent over its 15-year history as a direct result of the technology's success.

  1. Real Time PCR: Principles and Application

    Directory of Open Access Journals (Sweden)

    Safie Amini

    2005-09-01

    Full Text Available The polymerase chain reaction (PCR has been used as the new golden standard for detecting a wide variety of templates across a range of scientific specialties and also as an essential tool in research laboratories. PCR has completely revolutionized the detection of RNA and DNA viruses(1. Real Time vs. Traditional PCRReal time chemistry allows the detection of PCR amplification during the early phase of the reaction. Measuring the kinetic of the reaction in the early phase of PCR provides a distinct advantage over traditional PCR detection. Traditional methods use agarose gel electrophoresis for detection of PCR amplification at the final phase or end point. End point detection is really time consuming; it takes several hours to have the result. On the other hand, results are based on size discrimination. Also, the result of end point is variable from sample to sample. While gels may not resolve this variability in yield, real time PCR is sensitive enough to detect this change.Some problems with end point detection are: poor precision, low sensitivity, short dynamic range (<2 log, low resolution, non-automated procedure, size-based discrimination only, and post PCR processing (carry-over contamination and results are not expressed as numbers(2.Detection of PCR Products in Real-timeReal-time PCR and RT-PCR allow accurate quantification of starting amounts of DNA, cDNA, and RNA targets. Fluorescence is measured during each cycle, which greatly increases the dynamic range of the reaction since the amount offluorescence is proportional to the amount of PCR product. PCR products can be detected using either fluorescent dyes that bind to double-stranded DNA or fluorescently labeled sequence-specific probes(3.SYBR Green ISYBR® Green I binds all double-stranded DNA molecules, emitting a fluorescent signal of a defined wavelength on binding. The excitation and emission maxima of SYBR Green I are at 494 nm and 521 nm, respectively, and are compatible for

  2. Integrated real-time roof monitoring

    Institute of Scientific and Technical Information of China (English)

    SHEN Bao-tang; GUO Hua; KING Andrew

    2009-01-01

    CSIRO has recently developed a real-time roof monitoring system for under-ground coal mines and successfully tried the system in gate roads at Ulan Mine. The sys-tem integrated displacement monitoring, stress monitoring and seismic monitoring in one package. It included GEL multianchor extensometers, vibrating wire uniaxial stress meters, ESG seismic monitoring system with microseismic sensors and high-frequency AE sen-sors. The monitoring system automated and the data can be automatically collected by a central computer located in an underground nonhazardous area. The data are then trans-ferred to the surface via an optical fiber cable. The real-time data were accessed at any location with an Internet connection. The trials of the system in two tailgates at Ulan Mine demonstrate that the system is effective for monitoring the behavior and stability of read-ways during Iongwall mining. The continuous roof displacement/stress data show clear precursors of roof falls. The seismic data (event count and locations) provide insights into the roof failure process during roof fall.

  3. Video Surveillance for Real Time Objects

    Directory of Open Access Journals (Sweden)

    N.R. Raajan

    2012-12-01

    Full Text Available In this study, we describe an integrated solution for video surveillance in a fortified environment. The focus of this study is on identification of real time objects on different environments. The system is composed of robust object detection module, which normally detects the presence of abandoned objects, concealed objects hidden inside the human clothing, objects in dark environment and performs image segmentation with the intention of facilitating human operator’s task of retrieving the cause of a buzzer. The abandoned objects are detected by image segmentation based on temporal rank order filtering. Image fusion technique which fuses a color visual image and a corresponding IR image for concealed objects in guarded environment and in some cases like dark environment heat signature can be used for detecting real time objects etc. In the clips of interest, the key frame is the one depicting a person leaving a dangerous object and is determined on the basis of a feature indicating the movement around the dangerous region.

  4. Real-time PCR in microfluidic devices

    Science.gov (United States)

    Becker, Holger; Hlawatsch, Nadine; Klemm, Richard; Moche, Christian; Hansen-Hagge, Thomas; Gärtner, Claudia

    2014-03-01

    A central method in a standard biochemical laboratory is represented by the polymerase chain reaction (PCR), therefore many attempts have been performed so far to implement this technique in lab-on-a-chip (LOC) devices. PCR is an ideal candidate for miniaturization because of a reduction of assay time and decreased costs for expensive bio-chemicals. In case of the "classical" PCR, detection is done by identification of DNA fragments electrophoretically separated in agarose gels. This method is meanwhile frequently replaced by the so-called Real-Time-PCR because here the exponential increase of amplificates can be observed directly by measurement of DNA interacting fluorescent dyes. Two main methods for on-chip PCRs are available: traditional "batch" PCR in chambers on a chip using thermal cycling, requiring about 30 minutes for a typical PCR protocol and continuous-flow PCR, where the liquid is guided over stationary temperature zones. In the latter case, the PCR protocol can be as fast as 5 minutes. In the presented work, a proof of concept is demonstrated for a real-time-detection of PCR products in microfluidic systems.

  5. An efficient real time superresolution ASIC system

    Science.gov (United States)

    Reddy, Dikpal; Yue, Zhanfeng; Topiwala, Pankaj

    2008-04-01

    Superresolution of images is an important step in many applications like target recognition where the input images are often grainy and of low quality due to bandwidth constraints. In this paper, we present a real-time superresolution application implemented in ASIC/FPGA hardware, and capable of 30 fps of superresolution by 16X in total pixels. Consecutive frames from the video sequence are grouped and the registered values between them are used to fill the pixels in the higher resolution image. The registration between consecutive frames is evaluated using the algorithm proposed by Schaum et al. The pixels are filled by averaging a fixed number of frames associated with the smallest error distances. The number of frames (the number of nearest neighbors) is a user defined parameter whereas the weights in the averaging process are decided by inverting the corresponding smallest error distances. Wiener filter is used to post process the image. Different input parameters, such as size of input image, enlarging factor and the number of nearest neighbors, can be tuned conveniently by the user. We use a maximum word size of 32 bits to implement the algorithm in Matlab Simulink as well as the hardware, which gives us a fine balance between the number of bits and performance. The algorithm performs with real time speed with very impressive superresolution results.

  6. Real-time applications of neural nets

    Energy Technology Data Exchange (ETDEWEB)

    Spencer, J.E.

    1989-05-01

    Producing, accelerating and colliding very high power, low emittance beams for long periods is a formidable problem in real-time control. As energy has grown exponentially in time so has the complexity of the machines and their control systems. Similar growth rates have occurred in many areas, e.g., improved integrated circuits have been paid for with comparable increases in complexity. However, in this case, reliability, capability and cost have improved due to reduced size, high production and increased integration which allow various kinds of feedback. In contrast, most large complex systems (LCS) are perceived to lack such possibilities because only one copy is made. Neural nets, as a metaphor for LCS, suggest ways to circumvent such limitations. It is argued that they are logically equivalent to multi-loop feedback/forward control of faulty systems. While complimentary to AI, they mesh nicely with characteristics desired for real-time systems. Such issues are considered, examples given and possibilities discussed. 21 refs., 6 figs.

  7. ROBUST MEMORY MANAGEMENT USING REAL TIME CONCEPTS

    Directory of Open Access Journals (Sweden)

    V. Karthikeyan

    2014-01-01

    Full Text Available Memory fragmentation is the development of a large number of separate free areas. Memory management in embedded systems demand effective implementation schemes to avoid fragmentation problem. Existing dynamic memory allocation methods fail to suit real time system requirements. Execution times need to be deterministic and this motivates the need for allocation and deallocation to be done in constant time with the help of API’s. In µC/OS-II, memory allocation is semi-dynamic and a buddy allocator dynamic memory allocation algorithm is commonly used. Programmer must statically allocate a memory and partition the region using µC/OS-II Kernel API. Tasks can only request pre-partitioned fixed-size memory space from µC/OS-II. Memory allocation times are influenced by the ratio of memory allocation to the stack size of the task. In this research work memory management in LPC 1768 environment using RTOS µC/OS-II is proposed. Effective sharing of memory blocks among tasks co exists with partition. The captured results shows that the memory allocation and deallocation suits real time. The implication of the work is that, the necessity to reserve a static set of locations ahead of time is eliminated so that memory can be allocated at compile or design time.

  8. Memory controllers for real-time embedded systems predictable and composable real-time systems

    CERN Document Server

    Akesson, Benny

    2012-01-01

      Verification of real-time requirements in systems-on-chip becomes more complex as more applications are integrated. Predictable and composable systems can manage the increasing complexity using formal verification and simulation.  This book explains the concepts of predictability and composability and shows how to apply them to the design and analysis of a memory controller, which is a key component in any real-time system. This book is generally intended for readers interested in Systems-on-Chips with real-time applications.   It is especially well-suited for readers looking to use SDRAM memories in systems with hard or firm real-time requirements. There is a strong focus on real-time concepts, such as predictability and composability, as well as a brief discussion about memory controller architectures for high-performance computing. Readers will learn step-by-step how to go from an unpredictable SDRAM memory, offering highly variable bandwidth and latency, to a predictable and composable shared memory...

  9. Real-Time and Near Real-Time Data for Space Weather Applications and Services

    Science.gov (United States)

    Singer, H. J.; Balch, C. C.; Biesecker, D. A.; Matsuo, T.; Onsager, T. G.

    2015-12-01

    Space weather can be defined as conditions in the vicinity of Earth and in the interplanetary environment that are caused primarily by solar processes and influenced by conditions on Earth and its atmosphere. Examples of space weather are the conditions that result from geomagnetic storms, solar particle events, and bursts of intense solar flare radiation. These conditions can have impacts on modern-day technologies such as GPS or electric power grids and on human activities such as astronauts living on the International Space Station or explorers traveling to the moon or Mars. While the ultimate space weather goal is accurate prediction of future space weather conditions, for many applications and services, we rely on real-time and near-real time observations and model results for the specification of current conditions. In this presentation, we will describe the space weather system and the need for real-time and near-real time data that drive the system, characterize conditions in the space environment, and are used by models for assimilation and validation. Currently available data will be assessed and a vision for future needs will be given. The challenges for establishing real-time data requirements, as well as acquiring, processing, and disseminating the data will be described, including national and international collaborations. In addition to describing how the data are used for official government products, we will also give examples of how these data are used by both the public and private sector for new applications that serve the public.

  10. A compact multifunctional microfluidic platform for exploring cellular dynamics in real-time using electrochemical detection

    DEFF Research Database (Denmark)

    Zor, Kinga; Heiskanen, Arto; Caviglia, Claudia

    2014-01-01

    and electrochemical analysis platform with in-built fluid handling and detection, enabling complete cell based assays comprising on-line electrode cleaning, sterilization, surface functionalization, cell seeding, cultivation and electrochemical real-time monitoring of cellular dynamics. To demonstrate the versatility...

  11. Real-time forecasts of dengue epidemics

    Science.gov (United States)

    Yamana, T. K.; Shaman, J. L.

    2015-12-01

    Dengue is a mosquito-borne viral disease prevalent in the tropics and subtropics, with an estimated 2.5 billion people at risk of transmission. In many areas with endemic dengue, disease transmission is seasonal but prone to high inter-annual variability with occasional severe epidemics. Predicting and preparing for periods of higher than average transmission is a significant public health challenge. Here we present a model of dengue transmission and a framework for optimizing model simulations with real-time observational data of dengue cases and environmental variables in order to generate ensemble-based forecasts of the timing and severity of disease outbreaks. The model-inference system is validated using synthetic data and dengue outbreak records. Retrospective forecasts are generated for a number of locations and the accuracy of these forecasts is quantified.

  12. A Flexible Real-Time Architecture

    Energy Technology Data Exchange (ETDEWEB)

    WICKSTROM,GREGORY L.

    2000-08-17

    Assuring hard real-time characteristics of I/O associated with embedded software is often a difficult task. Input-Output related statements are often intermixed with the computational code, resulting in I/O timing that is dependent on the execution path and computational load. One way to mitigate this problem is through the use of interrupts. However, the non-determinism that is introduced by interrupt driven I/O may be so difficult to analyze that it is prohibited in some high consequence systems. This paper describes a balanced hardware/software solution to obtain consistent interrupt-free I/O timing, and results in software that is much more amenable to analysis.

  13. Embedded and real-time operating systems

    CERN Document Server

    Wang, K C

    2017-01-01

    This book covers the basic concepts and principles of operating systems, showing how to apply them to the design and implementation of complete operating systems for embedded and real-time systems. It includes all the foundational and background information on ARM architecture, ARM instructions and programming, toolchain for developing programs, virtual machines for software implementation and testing, program execution image, function call conventions, run-time stack usage and link C programs with assembly code. It describes the design and implementation of a complete OS for embedded systems in incremental steps, explaining the design principles and implementation techniques. For Symmetric Multiprocessing (SMP) embedded systems, the author examines the ARM MPcore processors, which include the SCU and GIC for interrupts routing and interprocessor communication and synchronization by Software Generated Interrupts (SGIs). Throughout the book, complete working sample systems demonstrate the design principles and...

  14. Kalman Filtering with Real-Time Applications

    CERN Document Server

    Chui, Charles K

    2009-01-01

    Kalman Filtering with Real-Time Applications presents a thorough discussion of the mathematical theory and computational schemes of Kalman filtering. The filtering algorithms are derived via different approaches, including a direct method consisting of a series of elementary steps, and an indirect method based on innovation projection. Other topics include Kalman filtering for systems with correlated noise or colored noise, limiting Kalman filtering for time-invariant systems, extended Kalman filtering for nonlinear systems, interval Kalman filtering for uncertain systems, and wavelet Kalman filtering for multiresolution analysis of random signals. Most filtering algorithms are illustrated by using simplified radar tracking examples. The style of the book is informal, and the mathematics is elementary but rigorous. The text is self-contained, suitable for self-study, and accessible to all readers with a minimum knowledge of linear algebra, probability theory, and system engineering.

  15. Feedback as real-time constructions

    DEFF Research Database (Denmark)

    Keiding, Tina Bering; Qvortrup, Ane

    2014-01-01

    This article offers a re-description of feedback and the significance of time in feedback constructions based on systems theory. It describes feedback as internal, real-time constructions in a learning system. From this perspective, feedback is neither immediate nor delayed, but occurs in the very...... instant it takes place. This article argues for a clear distinction between the timing of communicative events, such as responses that are provided as help for feedback constructions, and the feedback construction itself as an event in a psychic system. Although feedback is described as an internal......, system-relative construction, different teaching environments offer diverse conditions for feedback constructions. The final section of this article explores this idea with the help of examples from both synchronous oral interaction and asynchronous text-based interaction mediated by digital media....

  16. Real-Time Optical Antimicrobial Susceptibility Testing

    DEFF Research Database (Denmark)

    Fredborg, Marlene; Andersen, Klaus R; Jørgensen, Erik;

    2013-01-01

    Rapid antibiotic susceptibility testing is in highly demand in health-care fields as antimicrobial resistant bacterial strains emerge and spread. Here we describe an optical screening system (oCelloScope), which based on time-lapse imaging of 96 bacteria-antibiotic combinations at a time, introdu......Rapid antibiotic susceptibility testing is in highly demand in health-care fields as antimicrobial resistant bacterial strains emerge and spread. Here we describe an optical screening system (oCelloScope), which based on time-lapse imaging of 96 bacteria-antibiotic combinations at a time......, introduces real-time detection of bacterial growth and antimicrobial susceptibility, with imaging material to support the automatically generated graphs. Automated antibiotic susceptibility tests of a monoculture showed statistically significant antibiotic effect within 6 minutes and within 30 minutes...

  17. Mobility and language change in real time

    DEFF Research Database (Denmark)

    Monka, Malene

    -mobile peers prior to being geographically and socially mobile (e.g. Andersson & Thelander 1994). In the presentation I discuss this question by presenting a real time panel-study of language change in 23 speakers from three municipalities in distinct dialect areas in Denmark. The language change of six mobile......Diachronic studies of the interrelationship between mobility and language change leave us with some unanswered questions of causation. The most important question is whether language change is caused by mobility, or if mobile informants mark themselves linguistically different than their non...... the non-mobile speakers in the old recordings, and that the degree of language change differs among the mobile informants from the three dialect areas. Based on the qualitative analyses I argue that differences in geographic and social orientation in the old recordings can explain differences between...

  18. Operational and real-time Business Intelligence

    Directory of Open Access Journals (Sweden)

    Daniela Ioana SANDU

    2008-01-01

    Full Text Available A key component of a company’s IT framework is a business intelligence (BI system. BI enables business users to report on, analyze and optimize business operations to reduce costs and increase revenues. Organizations use BI for strategic and tactical decision making where the decision-making cycle may span a time period of several weeks (e.g., campaign management or months (e.g., improving customer satisfaction.Competitive pressures coming from a very dynamic business environment are forcing companies to react faster to changing business conditions and customer requirements. As a result, there is now a need to use BI to help drive and optimize business operations on a daily basis, and, in some cases, even for intraday decision making. This type of BI is usually called operational business intelligence and real-time business intelligence.

  19. Real-time, face recognition technology

    Energy Technology Data Exchange (ETDEWEB)

    Brady, S.

    1995-11-01

    The Institute for Scientific Computing Research (ISCR) at Lawrence Livermore National Laboratory recently developed the real-time, face recognition technology KEN. KEN uses novel imaging devices such as silicon retinas developed at Caltech or off-the-shelf CCD cameras to acquire images of a face and to compare them to a database of known faces in a robust fashion. The KEN-Online project makes that recognition technology accessible through the World Wide Web (WWW), an internet service that has recently seen explosive growth. A WWW client can submit face images, add them to the database of known faces and submit other pictures that the system tries to recognize. KEN-Online serves to evaluate the recognition technology and grow a large face database. KEN-Online includes the use of public domain tools such as mSQL for its name-database and perl scripts to assist the uploading of images.

  20. Near real-time stereo vision system

    Science.gov (United States)

    Anderson, Charles H. (Inventor); Matthies, Larry H. (Inventor)

    1993-01-01

    The apparatus for a near real-time stereo vision system for use with a robotic vehicle is described. The system is comprised of two cameras mounted on three-axis rotation platforms, image-processing boards, a CPU, and specialized stereo vision algorithms. Bandpass-filtered image pyramids are computed, stereo matching is performed by least-squares correlation, and confidence ranges are estimated by means of Bayes' theorem. In particular, Laplacian image pyramids are built and disparity maps are produced from the 60 x 64 level of the pyramids at rates of up to 2 seconds per image pair. The first autonomous cross-country robotic traverses (of up to 100 meters) have been achieved using the stereo vision system of the present invention with all computing done onboard the vehicle. The overall approach disclosed herein provides a unifying paradigm for practical domain-independent stereo ranging.

  1. CONSIDERATIONS ON REAL TIME DATA WAREHOUSING (RTDW

    Directory of Open Access Journals (Sweden)

    Marius Bogdan DINU

    2014-05-01

    Full Text Available The RTDW concept originated in the early 2000s. By that time, computing power had increased to a level that was allowing extraction of data collections for reporting purposes. Such collections were used almost in real time and at speeds nearly comparable to what an operation system was capable to deliver. The main idea will be to eliminate some of the components of the classic extraction process which is basically the most costly factor less time - consuming. We anticipate that the following factors will be decisive: elimination of batch-type processes [1], data compression techniques, data capture techniques, ability to keep in cache a large volume of data, parallel processing, and data mining algorithms that can adapt to such applications.

  2. Real-time color holographic interferometry

    Science.gov (United States)

    Desse, Jean-Michel; Albe, Felix; Tribillon, Jean-Louis

    2002-09-01

    A new optical technique based on real-time color holographic interferometry has been developed for analyzing unsteady aerodynamic wakes in fluid mechanics or for measuring displacements and deformations in solid mechanics. The technique's feasibility is demonstrated here. It uses three coherent wavelengths produced simultaneously by a cw laser (mixed argon and krypton). Holograms are recorded on single-layer panchromatic silver halide (Slavich PFG 03C) plates. Results show the optical setup can be adjusted to obtain a uniform background color. The interference fringe pattern visualized is large and colored and exhibits a single central white fringe, which makes the zero order of the interferogram easy to identify. An application in a subsonic wind tunnel is presented, in which the unsteady wake past a cylinder is recorded at high rate.

  3. Terrestrial Real-Time Volcano Monitoring

    Science.gov (United States)

    Franke, M.

    2013-12-01

    As volcano monitoring involves more and different sensors from seismic to GPS receivers, from video and thermal cameras to multi-parameter probes measuring temperature, ph values and humidity in the ground and the air, it becomes important to design real-time networks that integrate and leverage the multitude of available parameters. In order to do so some simple principles need to be observed: a) a common time base for all measurements, b) a packetized general data communication protocol for acquisition and distribution, c) an open and well documented interface to the data permitting standard and emerging innovative processing, and d) an intuitive visualization platform for scientists and civil defense personnel. Although mentioned as simple principles, the list above does not necessarily lead to obvious solutions or integrated systems, which is, however, required to take advantage of the available data. Only once the different data streams are put into context to each other in terms of time and location can a broader view be obtained and additional information extracted. The presentation is a summary of currently available technologies and how they can achieve the goal of an integrated real-time volcano monitoring system. A common time base are standard for seismic and GPS networks. In different projects we extended this to video feeds and time-lapse photography. Other probes have been integrated with vault interface enclosures (VIE) as used in the Transportable Array (TA) of the USArray. The VIE can accommodate the sensors employed in volcano monitoring. The TA has shown that Antelope is a versatile and robust middleware. It provides the required packetized general communication protocol that is independent from the actual physical communication link leaving the network design to adopt appropriate and possible hybrid solutions. This applies for the data acquisition and the data/information dissemination providing both a much needed collaboration platform, as

  4. Real-Time Imaging of Quantum Entanglement

    CERN Document Server

    Fickler, Robert; Lapkiewicz, Radek; Ramelow, Sven; Zeilinger, Anton

    2013-01-01

    Quantum Entanglement - correlations between at least two systems that are stronger than classically explainable - is widely regarded as one of the most prominent features of quantum mechanics and quantum information science. Although, the creation of entanglement between two systems has become possible in laboratories, it has been out of the grasp of one of the most natural ways to investigate nature: direct visual observation. Here we show that modern imaging technology, namely a triggered intensified charge coupled device (ICCD) camera, is fast and sensitive enough to image in real-time the influence of the measurement of one photon on its entangled partner. To demonstrate the non-classicality of the measurements quantitatively from the registered intensity we develop a novel method to statistically analyze the image and precisely quantify the number of photons within a certain region. In addition, we show the high flexibility of our experimental setup in creating any desired spatial-mode entanglement, even...

  5. Wi-Fi real time location systems

    Science.gov (United States)

    Doll, Benjamin A.

    This thesis objective was to determine the viability of utilizing an untrained Wi-Fi. real time location system as a GPS alternative for indoor environments. Background. research showed that GPS is rarely able to penetrate buildings to provide reliable. location data. The benefit of having location information in a facility and how they might. be used for disaster or emergency relief personnel and their resources motivated this. research. A building was selected with a well-deployed Wi-Fi infrastructure and its. untrained location feature was used to determine the distance between the specified. test points and the system identified location. It was found that the average distance. from the test point throughout the facility was 14.3 feet 80% of the time. This fell within. the defined viable range and supported that an untrained Wi-Fi RTLS system could be a. viable solution for GPS's lack of availability indoors.

  6. Towards real time speckle controlled retinal photocoagulation

    Science.gov (United States)

    Bliedtner, Katharina; Seifert, Eric; Stockmann, Leoni; Effe, Lisa; Brinkmann, Ralf

    2016-03-01

    Photocoagulation is a laser treatment widely used for the therapy of several retinal diseases. Intra- and inter-individual variations of the ocular transmission, light scattering and the retinal absorption makes it impossible to achieve a uniform effective exposure and hence a uniform damage throughout the therapy. A real-time monitoring and control of the induced damage is highly requested. Here, an approach to realize a real time optical feedback using dynamic speckle analysis is presented. A 532 nm continuous wave Nd:YAG laser is used for coagulation. During coagulation, speckle dynamics are monitored by a coherent object illumination using a 633nm HeNe laser and analyzed by a CMOS camera with a frame rate up to 1 kHz. It is obvious that a control system needs to determine whether the desired damage is achieved to shut down the system in a fraction of the exposure time. Here we use a fast and simple adaption of the generalized difference algorithm to analyze the speckle movements. This algorithm runs on a FPGA and is able to calculate a feedback value which is correlated to the thermal and coagulation induced tissue motion and thus the achieved damage. For different spot sizes (50-200 μm) and different exposure times (50-500 ms) the algorithm shows the ability to discriminate between different categories of retinal pigment epithelial damage ex-vivo in enucleated porcine eyes. Furthermore in-vivo experiments in rabbits show the ability of the system to determine tissue changes in living tissue during coagulation.

  7. Real Time Eye Template Detection and Tracking

    Directory of Open Access Journals (Sweden)

    Richa Mehta

    2012-06-01

    Full Text Available There has been a growing interest in the field of facial expression recognition especially in the last two decades. An example of such a system is the improvement of driver carefulness and accident reduction. The driver’s face is tracked while he is driving and he is warned if there seems to be an alerting fact that can result in an accident such as sleepy eyes, or looking out of the road. Furthermore, with a facial feature tracker, it becomes possible to play a synthesized avatar so that it imitates the expressions of the performer. Human-Computer Interaction (HCI systems may also be enriched by a facial feature tracker. For a user who is incapable of using her hands, a facial expression controller may be a solution to send limited commands to a computer. Eye blinking is one of the prominent areas to solve many real world problems. The process of blink detection consists of two phases. These are eye tracking followed by detection of blink. The work that has been carried out for eye tracking only is not suitable for eye blink detection. Therefore some approaches had been proposed for eye tracking along with eyes blink detection. In this thesis, real time implementation is done to count number of eye blinks in an image sequence. At last after analyzing all these approaches some of the parameters we obtained on which better performance of eye blink detection algorithm depend. This project focuses on automatic eye blink detection in real time. The aim of this thesis is to count the number of eye blinks in a video. This project will be performed on a video database of the facial expressions.

  8. Real-time visualization of joint cavitation.

    Directory of Open Access Journals (Sweden)

    Gregory N Kawchuk

    Full Text Available Cracking sounds emitted from human synovial joints have been attributed historically to the sudden collapse of a cavitation bubble formed as articular surfaces are separated. Unfortunately, bubble collapse as the source of joint cracking is inconsistent with many physical phenomena that define the joint cracking phenomenon. Here we present direct evidence from real-time magnetic resonance imaging that the mechanism of joint cracking is related to cavity formation rather than bubble collapse. In this study, ten metacarpophalangeal joints were studied by inserting the finger of interest into a flexible tube tightened around a length of cable used to provide long-axis traction. Before and after traction, static 3D T1-weighted magnetic resonance images were acquired. During traction, rapid cine magnetic resonance images were obtained from the joint midline at a rate of 3.2 frames per second until the cracking event occurred. As traction forces increased, real-time cine magnetic resonance imaging demonstrated rapid cavity inception at the time of joint separation and sound production after which the resulting cavity remained visible. Our results offer direct experimental evidence that joint cracking is associated with cavity inception rather than collapse of a pre-existing bubble. These observations are consistent with tribonucleation, a known process where opposing surfaces resist separation until a critical point where they then separate rapidly creating sustained gas cavities. Observed previously in vitro, this is the first in-vivo macroscopic demonstration of tribonucleation and as such, provides a new theoretical framework to investigate health outcomes associated with joint cracking.

  9. Intelligent, Real-Time Problem Solving

    Science.gov (United States)

    1990-03-08

    feature-edges loop! do-point-set (point seed-point &~key (resolution (quote ’gc-cell-resolution’)) (Z ( gensym )) [Macro] (y ( gensym )) visited-point...returned. do-vertex-neighbors (neighbor connection vertex 8key (edge ( gensym ))) Vibody body [Macro] Iterate over the neighbors of ’vertex’. ’Vertex

  10. An Efficient Secure Real-Time Concurrency Control Protocol

    Institute of Scientific and Technical Information of China (English)

    XIAO Yingyuan; LIU Yunsheng; CHEN Xiangyang

    2006-01-01

    Secure real-time databases must simultaneously satisfy two requirements in guaranteeing data security and minimizing the missing deadlines ratio of transactions. However, these two requirements can conflict with each other and achieve one requirement is to sacrifice the other. This paper presents a secure real-time concurrency control protocol based on optimistic method. The concurrency control protocol incorporates security constraints in a real-time optimistic concurrency control protocol and makes a suitable tradeoff between security and real-time requirements by introducing secure influence factor and real-time influence factor. The experimental results show the concurrency control protocol achieves data security without degrading real-time performance significantly.

  11. RTMOD: Real-Time MODel evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Graziani, G; Galmarini, S. [Joint Research centre, Ispra (Italy); Mikkelsen, T. [Risoe National Lab., Wind Energy and Atmospheric Physics Dept. (Denmark)

    2000-01-01

    The 1998 - 1999 RTMOD project is a system based on an automated statistical evaluation for the inter-comparison of real-time forecasts produced by long-range atmospheric dispersion models for national nuclear emergency predictions of cross-boundary consequences. The background of RTMOD was the 1994 ETEX project that involved about 50 models run in several Institutes around the world to simulate two real tracer releases involving a large part of the European territory. In the preliminary phase of ETEX, three dry runs (i.e. simulations in real-time of fictitious releases) were carried out. At that time, the World Wide Web was not available to all the exercise participants, and plume predictions were therefore submitted to JRC-Ispra by fax and regular mail for subsequent processing. The rapid development of the World Wide Web in the second half of the nineties, together with the experience gained during the ETEX exercises suggested the development of this project. RTMOD featured a web-based user-friendly interface for data submission and an interactive program module for displaying, intercomparison and analysis of the forecasts. RTMOD has focussed on model intercomparison of concentration predictions at the nodes of a regular grid with 0.5 degrees of resolution both in latitude and in longitude, the domain grid extending from 5W to 40E and 40N to 65N. Hypothetical releases were notified around the world to the 28 model forecasters via the web on a one-day warning in advance. They then accessed the RTMOD web page for detailed information on the actual release, and as soon as possible they then uploaded their predictions to the RTMOD server and could soon after start their inter-comparison analysis with other modelers. When additional forecast data arrived, already existing statistical results would be recalculated to include the influence by all available predictions. The new web-based RTMOD concept has proven useful as a practical decision-making tool for realtime

  12. CRANS - CONFIGURABLE REAL-TIME ANALYSIS SYSTEM

    Science.gov (United States)

    Mccluney, K.

    1994-01-01

    In a real-time environment, the results of changes or failures in a complex, interconnected system need evaluation quickly. Tabulations showing the effects of changes and/or failures of a given item in the system are generally only useful for a single input, and only with regard to that item. Subsequent changes become harder to evaluate as combinations of failures produce a cascade effect. When confronted by multiple indicated failures in the system, it becomes necessary to determine a single cause. In this case, failure tables are not very helpful. CRANS, the Configurable Real-time ANalysis System, can interpret a logic tree, constructed by the user, describing a complex system and determine the effects of changes and failures in it. Items in the tree are related to each other by Boolean operators. The user is then able to change the state of these items (ON/OFF FAILED/UNFAILED). The program then evaluates the logic tree based on these changes and determines any resultant changes to other items in the tree. CRANS can also search for a common cause for multiple item failures, and allow the user to explore the logic tree from within the program. A "help" mode and a reference check provide the user with a means of exploring an item's underlying logic from within the program. A commonality check determines single point failures for an item or group of items. Output is in the form of a user-defined matrix or matrices of colored boxes, each box representing an item or set of items from the logic tree. Input is via mouse selection of the matrix boxes, using the mouse buttons to toggle the state of the item. CRANS is written in C-language and requires the MIT X Window System, Version 11 Revision 4 or Revision 5. It requires 78K of RAM for execution and a three button mouse. It has been successfully implemented on Sun4 workstations running SunOS, HP9000 workstations running HP-UX, and DECstations running ULTRIX. No executable is provided on the distribution medium; however

  13. Real time model for public transportation management

    Directory of Open Access Journals (Sweden)

    Ireneusz Celiński

    2014-03-01

    Full Text Available Background: The article outlines managing a public transportation fleet in the dynamic aspect. There are currently many technical possibilities of identifying demand in the transportation network. It is also possible to indicate legitimate basis of estimating and steering demand. The article describes a general public transportation fleet management concept based on balancing demand and supply. Material and methods: The presented method utilizes a matrix description of demand for transportation based on telemetric and telecommunication data. Emphasis was placed mainly on a general concept and not the manner in which data was collected by other researchers.  Results: The above model gave results in the form of a system for managing a fleet in real-time. The objective of the system is also to optimally utilize means of transportation at the disposal of service providers. Conclusions: The presented concept enables a new perspective on managing public transportation fleets. In case of implementation, the project would facilitate, among others, designing dynamic timetables, updated based on observed demand, and even designing dynamic points of access to public transportation lines. Further research should encompass so-called rerouting based on dynamic measurements of the characteristics of the transportation system.

  14. The Colliderscope: a real-time show

    CERN Multimedia

    Francesco Poppi

    2010-01-01

    Ninety-six LED lights distributed over the facade of the Niels Bohr Institute (NBI) in Blegdamsvej (Denmark) reproduce the actual signals coming from the Transition Radiation Detector (TRT) in ATLAS. Thanks to the Colliderscope, when a collision occurs below the ground in Geneva, people passing by in Blegdamsvej will be aware of it almost in real-time.   Niels Bohr Institute facade lit up to reflect the latest data from ATLAS-TRT . The pattern, intensity and duration of the Colliderscope’s flashes of light depend on the physical parameters of particles crossing the ATLAS TRT detector. “At the Colliderscope very little happens randomly”, explains Troels Petersen, a physicist at NBI and one of the people who conceived it. “Particularly interesting events, such as electrons, are shown by a bright light that remains on the facade for several seconds”. The Niels Bohr Institute has participated in the development of the TRT detector, and this is why t...

  15. Real-Time Principal-Component Analysis

    Science.gov (United States)

    Duong, Vu; Duong, Tuan

    2005-01-01

    A recently written computer program implements dominant-element-based gradient descent and dynamic initial learning rate (DOGEDYN), which was described in Method of Real-Time Principal-Component Analysis (NPO-40034) NASA Tech Briefs, Vol. 29, No. 1 (January 2005), page 59. To recapitulate: DOGEDYN is a method of sequential principal-component analysis (PCA) suitable for such applications as data compression and extraction of features from sets of data. In DOGEDYN, input data are represented as a sequence of vectors acquired at sampling times. The learning algorithm in DOGEDYN involves sequential extraction of principal vectors by means of a gradient descent in which only the dominant element is used at each iteration. Each iteration includes updating of elements of a weight matrix by amounts proportional to a dynamic initial learning rate chosen to increase the rate of convergence by compensating for the energy lost through the previous extraction of principal components. In comparison with a prior method of gradient-descent-based sequential PCA, DOGEDYN involves less computation and offers a greater rate of learning convergence. The sequential DOGEDYN computations require less memory than would parallel computations for the same purpose. The DOGEDYN software can be executed on a personal computer.

  16. Real-time DIRCM system modeling

    Science.gov (United States)

    Petersson, Mikael

    2004-12-01

    Directed infrared countermeasures (DIRCM) play an increasingly important role in electronic warfare to counteract threats posed by infrared seekers. The usefulness and performance of such countermeasures depend, for example, on atmospheric conditions (attenuation and turbulence) and platform vibrations, causing pointing and tracking errors for the laser beam and reducing the power transferred to the seeker aperture. These problems make it interesting to simulate the performance of a DIRCM system in order to understand how easy or difficult it is to counteract an approaching threat and evaluate limiting factors in various situations. This paper describes a DIRCM model that has been developed, including atmospheric effects such as attenuation and turbulence as well as closed loop tracking algorithms, where the retro reflex of the laser is used for the pointing control of the beam. The DIRCM model is part of a large simulation framework (EWSim), which also incorporates several descriptions of different seekers (e.g. reticle, rosette, centroid, nutating cross) and models of robot dynamics. Effects of a jamming laser on a specific threat can be readily verified by simulations within this framework. The duel between missile and countermeasure is simulated in near real-time and visualized graphically in 3D. A typical simulation with a reticle seeker jammed by a modulated laser is included in the paper.

  17. Real-Time Accumulative Computation Motion Detectors

    Directory of Open Access Journals (Sweden)

    Saturnino Maldonado-Bascón

    2009-12-01

    Full Text Available The neurally inspired accumulative computation (AC method and its application to motion detection have been introduced in the past years. This paper revisits the fact that many researchers have explored the relationship between neural networks and finite state machines. Indeed, finite state machines constitute the best characterized computational model, whereas artificial neural networks have become a very successful tool for modeling and problem solving. The article shows how to reach real-time performance after using a model described as a finite state machine. This paper introduces two steps towards that direction: (a A simplification of the general AC method is performed by formally transforming it into a finite state machine. (b A hardware implementation in FPGA of such a designed AC module, as well as an 8-AC motion detector, providing promising performance results. We also offer two case studies of the use of AC motion detectors in surveillance applications, namely infrared-based people segmentation and color-based people tracking, respectively.

  18. Recommendations for Real-Time Speech MRI

    Science.gov (United States)

    Lingala, Sajan Goud; Sutton, Brad P.; Miquel, Marc E.; Nayak, Krishna S.

    2016-01-01

    Real-time magnetic resonance imaging (RT-MRI) is being increasingly used for speech and vocal production research studies. Several imaging protocols have emerged based on advances in RT-MRI acquisition, reconstruction, and audio-processing methods. This review summarizes the state-of-the-art, discusses technical considerations, and provides specific guidance for new groups entering this field. We provide recommendations for performing RT-MRI of the upper airway. This is a consensus statement stemming from the ISMRM-endorsed Speech MRI summit held in Los Angeles, February 2014. A major unmet need identified at the summit was the need for consensus on protocols that can be easily adapted by researchers equipped with conventional MRI systems. To this end, we provide a discussion of tradeoffs in RT-MRI in terms of acquisition requirements, a priori assumptions, artifacts, computational load, and performance for different speech tasks. We provide four recommended protocols and identify appropriate acquisition and reconstruction tools. We list pointers to open-source software that facilitate implementation. We conclude by discussing current open challenges in the methodological aspects of RT-MRI of speech. PMID:26174802

  19. Real-time optoacoustic monitoring of stroke

    Science.gov (United States)

    Kneipp, Moritz; Turner, Jake; Hambauer, Sebastian; Krieg, Sandro M.; Lehmberg, Jens; Lindauer, Ute; Razansky, Daniel

    2014-03-01

    Characterizing disease progression and identifying possible therapeutic interventions in stroke is greatly aided by the use of longitudinal function imaging studies. In this study, we investigate the applicability of real-time multispectral optoacoustic tomography (MSOT) as a tool for non-invasive monitoring of the progression of stroke in the whole brain. The middle cerebral artery occlusion (MCAO) method was used to induce stroke. Mice were imaged under isoflurane anesthesia preoperatively and at several time points during and after the 60-minute occlusion. The animals were sacrificed after 24 hours and their excised brains frozen at -80°C for sectioning. The cryosection were stained using H&E staining to identify the ischemic lesion. Major vessels are readily identifiable in the whole mouse head in the in vivo optoacoustic scans. During ischemia, a reduction in cerebral blood volume is detectable in the cortex. Post ischemia, spectral unmixing of the optoacoustic signals shows an asymmetry of the deoxygenated hemoglobin in the hemisphere affected by MCAO. This hypoxic area was mainly located around the boundary of the ischemic lesion and was therefore identified as the ischemic penumbra. Non-invasive functional MSOT imaging is able to visualize the hypoxic penumbra in brains affected by stroke. Stopping the spread of the infarct area and revitalizing the penumbra is central in stroke research, this new imaging technique may therefore prove to be a valuable tool in the monitoring and developing new treatments.

  20. Business Hypervisors for Real-time Applications

    Directory of Open Access Journals (Sweden)

    L. Perneel

    2015-08-01

    Full Text Available System virtualization is one of the hottest trends in information technology today. It is not just another nice to use technology but has become fundamental across the business world. It is successfully used with many business application classes where cloud computing is the most visual one. Recently, it started to be used for soft Real-Time (RT applications such as IP telephony, media servers, audio and video streaming servers, automotive and communication systems in general. Running these applications on a traditional system (Hardware + Operating System guarantee their Quality of Service (QoS; virtualizing them means inserting a new layer between the hardware and the (virtual Operating System (OS, and thus adding extra overhead. Although these applications’ areas do not always demand hard time guarantees, they require the underlying virtualization layer supports low latency and provide adequate computational resources for completion within a reasonable or predictable timeframe. These aspects are intimately intertwined with the logic of the hypervisor scheduler. In this paper, a series of tests are conducted on three hypervisors (VMware ESXi, Hyper-V server and Xen to provide a benchmark of the latencies added to the applications running on top of them. These tests are conducted for different scenarios (use cases to take into consideration all the parameters and configurations of the hypervisors’ schedulers. Finally, this benchmark can be used as a reference for choosing the best hypervisor-application combination.

  1. Real-Time 3D Visualization

    Science.gov (United States)

    1997-01-01

    Butler Hine, former director of the Intelligent Mechanism Group (IMG) at Ames Research Center, and five others partnered to start Fourth Planet, Inc., a visualization company that specializes in the intuitive visual representation of dynamic, real-time data over the Internet and Intranet. Over a five-year period, the then NASA researchers performed ten robotic field missions in harsh climes to mimic the end- to-end operations of automated vehicles trekking across another world under control from Earth. The core software technology for these missions was the Virtual Environment Vehicle Interface (VEVI). Fourth Planet has released VEVI4, the fourth generation of the VEVI software, and NetVision. VEVI4 is a cutting-edge computer graphics simulation and remote control applications tool. The NetVision package allows large companies to view and analyze in virtual 3D space such things as the health or performance of their computer network or locate a trouble spot on an electric power grid. Other products are forthcoming. Fourth Planet is currently part of the NASA/Ames Technology Commercialization Center, a business incubator for start-up companies.

  2. Real time visual servoing using controlled illumination

    Science.gov (United States)

    Urban, J. P.; Motyl, G.; Gallice, J.

    1994-02-01

    A real-time visual servoing approach is applied to robotics tasks consisting of the positioning of the end effector with respect to a priori known polyhedral objects. The vision apparatus is consituted by a compact CCD camera rigidly coupled with two laser stripes mounted on the wrist of a robot manipulator. The objective is to servo the robot and effector at a constant position and orientation with respect to a known object in three- dimensional space in the field of view of the sensory system. The approach is expressed in terms of sensor-based control applied to visual servoing. In the case of camera-light stripe coupling, the elementary visual signals used for visual servoing are the points of discontinuity in the light stripes. The feasibility of the approach is demonstrated in a factory automation task consisting of the positioning of the end-effector tool over a vehicle battery. Both simulation and experimentation results are presented, proving the robustness and stability of the algorithm.

  3. Internet-accessible real-time weather information system

    Digital Repository Service at National Institute of Oceanography (India)

    Desai, R.G.P.; Joseph, A.; Desa, E.; Mehra, P.; Desa, E.; Gouveia, A.D.

    An internet-accessible real-time weather information system has been developed. This system provides real-time accessibility to weather information from a multitude of spatially distributed weather stations. The Internet connectivity also offers...

  4. A real-time data acquisition and elaboration system for instabilities control in the FTU tokamak

    Energy Technology Data Exchange (ETDEWEB)

    Alessi, E., E-mail: alessi@ifp.cnr.it [Associazione EURATOM-ENEA, IFP-CNR, Milano (Italy); Boncagni, L. [Associazione EURATOM-ENEA, C.R. Frascati (Italy); Galperti, C.; Marchetto, C.; Nowak, S.; Sozzi, C. [Associazione EURATOM-ENEA, IFP-CNR, Milano (Italy); Apruzzese, G. [Associazione EURATOM-ENEA, C.R. Frascati (Italy); Bin, W. [Associazione EURATOM-ENEA, IFP-CNR, Milano (Italy); Belli, F.; Botrugno, A. [Associazione EURATOM-ENEA, C.R. Frascati (Italy); Bruschi, A.; Cirant, S. [Associazione EURATOM-ENEA, IFP-CNR, Milano (Italy); D' Antona, G.; Davoudi, M. [Politecnico di Milano, Dipartimento di Elettrotecnica (Italy); Figini, L. [Associazione EURATOM-ENEA, IFP-CNR, Milano (Italy); Ferrero, R. [Politecnico di Milano, Dipartimento di Elettrotecnica (Italy); Gabellieri, L. [Associazione EURATOM-ENEA, C.R. Frascati (Italy); Garavaglia, S.; Granucci, G. [Associazione EURATOM-ENEA, IFP-CNR, Milano (Italy); Grosso, A. [Associazione EURATOM-ENEA, C.R. Frascati (Italy); and others

    2013-08-21

    A real-time data acquisition and elaboration system is being implemented to control the new ECH launcher recently installed at FTU (Frascati Tokamak Upgrade). The system is aimed at controlling different kinds of magnetohydrodynamic instabilities, in particular the deleterious 3/2 and 2/1 (neoclassical) tearing modes, (N)TM, and the saw teeth period in order to prevent the seeding of NTMs. The complete system is presented here together with preliminary offline and real-time tests. © 2001 Elsevier Science. All rights reserved.

  5. Brewing with fractionated barley

    OpenAIRE

    Donkelaar, van, CC René

    2016-01-01

    Brewing with fractionated barley Beer is a globally consumed beverage, which is produced from malted barley, water, hops and yeast. In recent years, the use of unmalted barley and exogenous enzymes have become more popular because they enable simpler processing and reduced environmental impact. Raw barley, however, contains less endogenous enzymes and more undesired components for the use of beer brewing, compared to malted barley.  The overall aim of this thesis is to investigate how ba...

  6. Execution of a High Level Real-Time Language

    OpenAIRE

    Luqi; Berzins, Valdis

    1988-01-01

    Prototype System Description Language (PSDL) is a high level real-time language with special features for hard real-time system specification and design. It can be used to firm up requirements through execution of its software prototypes The language is designed based on a real-time model merging data and control flow and its implementation is beyond conventional compiler technology because of the need to meet real-time constraints. In this paper we describe and illustrate our research result...

  7. Survey of real-time processing systems for big data

    DEFF Research Database (Denmark)

    Liu, Xiufeng; Lftikhar, Nadeem; Xie, Xike

    2014-01-01

    for big data; Its open-source implementation such as Hadoop has become the de-facto standard for processing big data, however, Hadoop has the limitation of supporting real-time updates. The improvements in Hadoop for the real-time capability, and the other alternative real-time frameworks have been...

  8. Real-Time Gender Classification by Face

    Directory of Open Access Journals (Sweden)

    Eman Fares Al Mashagba

    2016-03-01

    Full Text Available The identification of human beings based on their biometric body parts, such as face, fingerprint, gait, iris, and voice, plays an important role in electronic applications and has become a popular area of research in image processing. It is also one of the most successful applications of computer–human interaction and understanding. Out of all the abovementioned body parts,the face is one of most popular traits because of its unique features.In fact, individuals can process a face in a variety of ways to classify it by its identity, along with a number of other characteristics, such as gender, ethnicity, and age. Specifically, recognizing human gender is important because people respond differently according to gender. In this paper, we present a robust method that uses global geometry-based features to classify gender and identify age and human beings from video sequences. The features are extracted based on face detection using skin color segmentation and the computed geometric features of the face ellipse region. These geometric features are then used to form the face vector trajectories, which are inputted to a time delay neural network and are trained using the Broyden–Fletcher–Goldfarb–Shanno (BFGS function. Results show that using the suggested method with our own dataset under an unconstrained condition achieves a 100% classification rate in the training set for all application, as well as 91.2% for gender classification, 88% for age identification, and 83% for human identification in the testing set. In addition, the proposed method establishes the real-time system to be used in three applications with a simple computation for feature extraction.

  9. Real time PV manufacturing diagnostic system

    Energy Technology Data Exchange (ETDEWEB)

    Kochergin, Vladimir [MicroXact Inc., Blacksburg, VA (United States); Crawford, Michael A. [MicroXact Inc., Blacksburg, VA (United States)

    2015-09-01

    The main obstacle Photovoltaic (PV) industry is facing at present is the higher cost of PV energy compared to that of fossil energy. While solar cell efficiencies continue to make incremental gains these improvements are so far insufficient to drive PV costs down to match that of fossil energy. Improved in-line diagnostics however, has the potential to significantly increase the productivity and reduce cost by improving the yield of the process. On this Phase I/Phase II SBIR project MicroXact developed and demonstrated at CIGS pilot manufacturing line a high-throughput in-line PV manufacturing diagnostic system, which was verified to provide fast and accurate data on the spatial uniformity of thickness, an composition of the thin films comprising the solar cell as the solar cell is processed reel-to-reel. In Phase II project MicroXact developed a stand-alone system prototype and demonstrated the following technical characteristics: 1) ability of real time defect/composition inconsistency detection over 60cm wide web at web speeds up to 3m/minute; 2) Better than 1mm spatial resolution on 60cm wide web; 3) an average better than 20nm spectral resolution resulting in more than sufficient sensitivity to composition imperfections (copper-rich and copper-poor regions were detected). The system was verified to be high vacuum compatible. Phase II results completely validated both technical and economic feasibility of the proposed concept. MicroXact’s solution is an enabling technique for in-line PV manufacturing diagnostics to increase the productivity of PV manufacturing lines and reduce the cost of solar energy, thus reducing the US dependency on foreign oil while simultaneously reducing emission of greenhouse gasses.

  10. Comprehensive GMO detection using real-time PCR array: single-laboratory validation.

    Science.gov (United States)

    Mano, Junichi; Harada, Mioko; Takabatake, Reona; Furui, Satoshi; Kitta, Kazumi; Nakamura, Kosuke; Akiyama, Hiroshi; Teshima, Reiko; Noritake, Hiromichi; Hatano, Shuko; Futo, Satoshi; Minegishi, Yasutaka; Iizuka, Tayoshi

    2012-01-01

    We have developed a real-time PCR array method to comprehensively detect genetically modified (GM) organisms. In the method, genomic DNA extracted from an agricultural product is analyzed using various qualitative real-time PCR assays on a 96-well PCR plate, targeting for individual GM events, recombinant DNA (r-DNA) segments, taxon-specific DNAs, and donor organisms of the respective r-DNAs. In this article, we report the single-laboratory validation of both DNA extraction methods and component PCR assays constituting the real-time PCR array. We selected some DNA extraction methods for specified plant matrixes, i.e., maize flour, soybean flour, and ground canola seeds, then evaluated the DNA quantity, DNA fragmentation, and PCR inhibition of the resultant DNA extracts. For the component PCR assays, we evaluated the specificity and LOD. All DNA extraction methods and component PCR assays satisfied the criteria set on the basis of previous reports.

  11. A Real-Time Lane Detection Algorithm Based on Intelligent CCD Parameters Regulation

    Directory of Open Access Journals (Sweden)

    Ping-shu Ge

    2012-01-01

    Full Text Available Lane departure warning system (LDWS has been regarded as an efficient method to lessen the damages of road traffic accident resulting from driver fatigue or inattention. Lane detection is one of the key techniques for LDWS. To overcome the contradiction between complexity of algorithm and the real-time requirement for vehicle onboard system, this paper introduces a new lane detection method based on intelligent CCD parameters regulation. In order to improve the real-time capability of the system, a CCD parameters regulating method is proposed which enhances the contrast between lane line and road surfaces and reduces image noise, so it lays a good foundation for the following lane detection. Hough transform algorithm is improved by selection and classification of seed points. Finally the lane line is extracted through some restrictions. Experimental results verify the effectiveness of the proposed method, which improves not only real-time capability but also the accuracy of the system.

  12. Real-time Forensic Disaster Analysis

    Science.gov (United States)

    Wenzel, F.; Daniell, J.; Khazai, B.; Mühr, B.; Kunz-Plapp, T.; Markus, M.; Vervaeck, A.

    2012-04-01

    The Center for Disaster Management and Risk Reduction Technology (CEDIM, www.cedim.de) - an interdisciplinary research center founded by the German Research Centre for Geoscience (GFZ) and Karlsruhe Institute of Technology (KIT) - has embarked on a new style of disaster research known as Forensic Disaster Analysis. The notion has been coined by the Integrated Research on Disaster Risk initiative (IRDR, www.irdrinternational.org) launched by ICSU in 2010. It has been defined as an approach to studying natural disasters that aims at uncovering the root causes of disasters through in-depth investigations that go beyond the reconnaissance reports and case studies typically conducted after disasters. In adopting this comprehensive understanding of disasters CEDIM adds a real-time component to the assessment and evaluation process. By comprehensive we mean that most if not all relevant aspects of disasters are considered and jointly analysed. This includes the impact (human, economy, and infrastructure), comparisons with recent historic events, social vulnerability, reconstruction and long-term impacts on livelihood issues. The forensic disaster analysis research mode is thus best characterized as "event-based research" through systematic investigation of critical issues arising after a disaster across various inter-related areas. The forensic approach requires (a) availability of global data bases regarding previous earthquake losses, socio-economic parameters, building stock information, etc.; (b) leveraging platforms such as the EERI clearing house, relief-web, and the many sources of local and international sources where information is organized; and (c) rapid access to critical information (e.g., crowd sourcing techniques) to improve our understanding of the complex dynamics of disasters. The main scientific questions being addressed are: What are critical factors that control loss of life, of infrastructure, and for economy? What are the critical interactions

  13. Instrumentation development for real time brainwave monitoring.

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, Lawrence Frederick; Clough, Benjamin W.

    2005-12-01

    The human brain functions through a chemically-induced biological process which operates in a manner similar to electrical systems. The signal resulting from this biochemical process can actually be monitored and read using tools and having patterns similar to those found in electrical and electronics engineering. The primary signature of this electrical activity is the ''brain wave'', which looks remarkably similar to the output of many electrical systems. Likewise, the device currently used in medical arenas to read brain electrical activity is the electroencephalogram (EEG) which is synonymous with a multi-channel oscilloscope reading. Brain wave readings and recordings for medical purposes are traditionally taken in clinical settings such as hospitals, laboratories or diagnostic clinics. The signal is captured via externally applied scalp electrodes using semi-viscous gel to reduce impedance. The signal will be in the 10 to 100 microvolt range. In other instances, where surgeons are attempting to isolate particular types of minute brain signals, the electrodes may actually be temporarily implanted in the brain during a preliminary procedure. The current configurations of equipment required for EEGs involve large recording instruments, many electrodes, wires, and large amounts of hard disk space devoted to storing large files of brain wave data which are then eventually analyzed for patterns of concern. Advances in sensors, signal processing, data storage and microelectronics over the last decade would seem to have paved the way for the realization of devices capable of ''real time'' external monitoring, and possible assessment, of brain activity. A myriad of applications for such a capability are likewise presenting themselves, including the ability to assess brain functioning, level of functioning and malfunctioning. Our plan is to develop the sensors, signal processing, and portable instrumentation package which could

  14. Real Time Wide Area Radiation Surveillance System

    Science.gov (United States)

    Biafore, M.

    2012-04-01

    We present the REWARD project, financed within the FP7 programme, theme SEC-2011.1.5-1 (Development of detection capabilities of difficult to detect radioactive sources and nuclear materials - Capability Project). Within this project, we propose a novel mobile system for real time, wide area radiation surveillance. The system is based on the integration of new miniaturized solid-state radiation sensors: a CdZnTe detector for gamma radiation and a high efficiency neutron detector based on novel silicon technologies. The sensing unit will include a wireless communication interface to send the data remotely to a monitoring base station which also uses a GPS system to calculate the position of the tag. The system will also incorporate middleware and high level software to provide web-service interfaces for the exchange of information, and that will offer top level functionalities as management of users, mobile tags and environment data and alarms, database storage and management and a web-based graphical user interface. Effort will be spent to ensure that the software is modular and re-usable across as many architectural levels as possible. Finally, an expert system will continuously analyze the information from the radiation sensor and correlate it with historical data from the tag location in order to generate an alarm when an abnormal situation is detected. The system will be useful for many different scenarios, including such lost radioactive sources and radioactive contamination. It will be possible to deploy in emergency units and in general in any type of mobile or static equipment. The sensing units will be highly portable thanks to their low size and low energy consumption. The complete system will be scalable in terms of complexity and cost and will offer very high precision on both the measurement and the location of the radiation. The modularity and flexibility of the system will allow for a realistic introduction to the market. Authorities may start with a

  15. Real time UAV autonomy through offline calculations

    Science.gov (United States)

    Jung, Sunghun

    . Once one or several targets are detected, UAVs near the target are manipulated to approach to the target. If the number of detected targets is more than one, UAVs are evenly grouped to track targets. After a specific period of time, UAVs hand off and continue their original tasks. Thirdly, Emergency algorithm is generated to avoid losses of UAVs when UAVs have system failures. If one UAV is out of fuel or control during the mission, the Emergency algorithm brings the malfunctioning UAV to the point of departure and let the rest UAVs to continue an aerial reconnaissance. An UAV which finishes its task the earliest will continue to search a region which the failed UAV is supposed to search. In addition, Emergency algorithm prevents UAVs colliding into each other by using emergency altitude. Overall, the framework developed here facilitates the solution of several mission planning problems. The robustness built into our discretization of space and time permits feedback corrections on real-time to vehicle trajectories. The library of off-line solutions proposed and developed here minimizes computational overhead during operations.

  16. Real Time Flux Control in PM Motors

    Energy Technology Data Exchange (ETDEWEB)

    Otaduy, P.J.

    2005-09-27

    Significant research at the Oak Ridge National Laboratory (ORNL) Power Electronics and Electric Machinery Research Center (PEEMRC) is being conducted to develop ways to increase (1) torque, (2) speed range, and (3) efficiency of traction electric motors for hybrid electric vehicles (HEV) within existing current and voltage bounds. Current is limited by the inverter semiconductor devices' capability and voltage is limited by the stator wire insulation's ability to withstand the maximum back-electromotive force (emf), which occurs at the upper end of the speed range. One research track has been to explore ways to control the path and magnitude of magnetic flux while the motor is operating. The phrase, real time flux control (RTFC), refers to this mode of operation in which system parameters are changed while the motor is operating to improve its performance and speed range. RTFC has potential to meet an increased torque demand by introducing additional flux through the main air gap from an external source. It can augment the speed range by diverting flux away from the main air gap to reduce back-emf at high speeds. Conventional RTFC technology is known as vector control [1]. Vector control decomposes the stator current into two components; one that produces torque and a second that opposes (weakens) the magnetic field generated by the rotor, thereby requiring more overall stator current and reducing the efficiency. Efficiency can be improved by selecting a RTFC method that reduces the back-emf without increasing the average current. This favors methods that use pulse currents or very low currents to achieve field weakening. Foremost in ORNL's effort to develop flux control is the work of J. S. Hsu. Early research [2,3] introduced direct control of air-gap flux in permanent magnet (PM) machines and demonstrated it with a flux-controlled generator. The configuration eliminates the problem of demagnetization because it diverts all the flux from the

  17. Cy5 maleimide labelling for sensitive detection of free thiols in native protein extracts: identification of seed proteins targeted by barley thioredoxin h isoforms

    DEFF Research Database (Denmark)

    Maeda, K.; Finnie, Christine; Svensson, Birte

    2004-01-01

    search. HvTrxh1 and HvTrxh2 were shown to have similar target specificity. Barley alpha-amylase/subtilisin inhibitor, previously demonstrated to be reduced by both HvTrxh1 and HvTrxh2, was among the identified target proteins, confirming the suitability of the method. Several alpha-amylase/trypsin...... inhibitors, some of which are already known as target proteins of thioredoxin h, and cyclophilin known as a target protein of m-type thioredoxin were also identified. Lipid transfer protein, embryo-specific protein, three chitinase isoenzymes, a single-domain glyoxalase-like protein and superoxide dismutase...

  18. RealTimeFrame – A Real Time Processing Framework for Medical Video Sequences

    Directory of Open Access Journals (Sweden)

    S. Gross

    2008-01-01

    Full Text Available Imaging technology is highly important in today’s medical environments. It provides information upon which the accuracy of the diagnosis and consequently the wellbeing of the patient rely. Increasing the quality and significance of medical image data is therefore one the aims of scientific research and development. We introduce an integrated hardware and software framework for real time image processing in medical environments, which we call RealTimeFrame. Our project is designed to offer flexibility, easy expandability and high performance. We use standard personal computer hardware to run our multithreaded software. A frame grabber card is used to capture video signals from medical imaging systems. A modular, user-defined process chain performs arbitrary manipulations on the image data. The graphical user interface offers configuration options and displays the processed image in either window or full screen mode. Image source and processing routines are encapsulated in dynamic library modules for easy functionality extension without recompilation of the entire software framework. Documented template modules for sources and processing steps are part of the software’s source code.

  19. A real-time transaction service for grid computing

    Institute of Scientific and Technical Information of China (English)

    Tang Feilong; Li Minglu; Joshua Huang Zhexue; Frank Tong; Cao Jian

    2005-01-01

    A real-time transaction service aims at ensuring the consistency of real-time and transactional Grid applications. This paper proposes an efficient real-time transaction service (RTTS). It provides abilities to discover qualified services, I.e., participants, to execute specified sub-transactions, coordinate real-time Grid transactions, calculate deadline and assign priority for scheduling concurrent transactions. The service discovery adopts the two-level registry mechanismto adapt to transient Grid services. The coordination algorithm invokes multiplefunctional alternative services for each sub-transaction so that it remarkablyimproves the successful ratio of real-time transactions. The experiment result shows that the RTTS can well coordinate real-time transactions for Grid service environment and shield users from the complex process.

  20. Advanced real-time manipulation of video streams

    CERN Document Server

    Herling, Jan

    2014-01-01

    Diminished Reality is a new fascinating technology that removes real-world content from live video streams. This sensational live video manipulation actually removes real objects and generates a coherent video stream in real-time. Viewers cannot detect modified content. Existing approaches are restricted to moving objects and static or almost static cameras and do not allow real-time manipulation of video content. Jan Herling presents a new and innovative approach for real-time object removal with arbitrary camera movements.

  1. Sentiment analysis on students’ real-time feedback

    OpenAIRE

    Altrabsheh, Nabeela

    2016-01-01

    Previous literature identifies that students’ real-time feedback is important in the learning process. There are numerous studies that have collected students’ feedback in real time. However, they include several limitations of which the most important is analysing the feedback. In this thesis, we address these limitations by proposing a system that will automatically analyse students’ feedback in real time and present the analysis results to the lecturer. To create such a system, we propose ...

  2. Real Time Synchronization for Creativity in Distributed Innovation Teams

    DEFF Research Database (Denmark)

    Peitersen, Dennis Kjaersgaard; Dolog, Peter; Pedersen, Esben Staunsbjerg

    2009-01-01

    In this paper we introduce a synchronization approach for real time collaborative sketching for creativity in distributed innovation teams. We base our approach on reverse AJAX. This way we ensure scalable solution for real time drawing and sketching important in creativity settings.......In this paper we introduce a synchronization approach for real time collaborative sketching for creativity in distributed innovation teams. We base our approach on reverse AJAX. This way we ensure scalable solution for real time drawing and sketching important in creativity settings....

  3. Unified Modeling of Complex Real-Time Control Systems

    CERN Document Server

    Hai, He; Chi-Lan, Cai

    2011-01-01

    Complex real-time control system is a software dense and algorithms dense system, which needs modern software engineering techniques to design. UML is an object-oriented industrial standard modeling language, used more and more in real-time domain. This paper first analyses the advantages and problems of using UML for real-time control systems design. Then, it proposes an extension of UML-RT to support time-continuous subsystems modeling. So we can unify modeling of complex real-time control systems on UML-RT platform, from requirement analysis, model design, simulation, until generation code.

  4. Building Real-Time Collaborative Applications with a Federated Architecture

    Directory of Open Access Journals (Sweden)

    Pablo Ojanguren-Menendez

    2015-12-01

    Full Text Available Real-time collaboration is being offered by multiple libraries and APIs (Google Drive Real-time API, Microsoft Real-Time Communications API, TogetherJS, ShareJS, rapidly becoming a mainstream option for webservices developers. However, they are offered as centralised services running in a single server, regardless if they are free/open source or proprietary software. After re-engineering Apache Wave (former Google Wave, we can now provide the first decentralised and federated free/open source alternative. The new API allows to develop new real-time collaborative web applications in both JavaScript and Java environments.

  5. Verifying real-time systems against scenario-based requirements

    DEFF Research Database (Denmark)

    Larsen, Kim Guldstrand; Li, Shuhao; Nielsen, Brian;

    2009-01-01

    subset of the LSC language. By equivalently translating an LSC chart into an observer TA and then non-intrusively composing this observer with the original system model, the problem of verifying a real-time system against a scenario-based requirement reduces to a classical real-time model checking......We propose an approach to automatic verification of real-time systems against scenario-based requirements. A real-time system is modeled as a network of Timed Automata (TA), and a scenario-based requirement is specified as a Live Sequence Chart (LSC). We define a trace-based semantics for a kernel...

  6. Brewing with fractionated barley

    NARCIS (Netherlands)

    Donkelaar, van L.H.G.

    2016-01-01

    Brewing with fractionated barley Beer is a globally consumed beverage, which is produced from malted barley, water, hops and yeast. In recent years, the use of unmalted barley and exogenous enzymes have become more popular because they enable simpler processing and reduced environmental impact. Raw

  7. PHYSIOLOGICAL AND AGROECOLOGICAL ASPECTS OF CADMIUM INTERACTIONS WITH BARLEY PLANTS: AN OVERVIEW

    Directory of Open Access Journals (Sweden)

    A VASSILEV

    2003-07-01

    Full Text Available This work is a review of author’s previous publications, unpublished results as well as available literature on barley responses to Cd contamination. The physiological backgrounds of the acute Cd toxicity in barley plants are briefly described. Some data characterizing the chronic Cd toxicity in barley have been also provided in relation to its possible use for seed production and Cd phytoextraction on Cd-contaminated agricultural soils. Information about the main physiological factors limiting growth of Cd-exposed barley plants and grain yield, seedling quality as well as Cd phytoextraction capacity of barley grown in Cd-contaminated soils is presented.

  8. Quantitation of viral load using real-time amplification techniques

    NARCIS (Netherlands)

    Niesters, H G

    2001-01-01

    Real-time PCR amplification techniques are currently used to determine the viral load in clinical samples for an increasing number of targets. Real-time PCR reduces the time necessary to generate results after amplification. In-house developed PCR and nucleic acid sequence-based amplification (NASBA

  9. "Real-Time" Case Studies in Organizational Communication

    Science.gov (United States)

    Long, Shawn D.

    2005-01-01

    This article presents an activity that integrates theory and application by examining the multiple communication events affecting a single organization in "real time" over the course of an academic term. The "real-time" case study (RTCS) avails students of the opportunity to examine organizational communication events as they are occurring in…

  10. 76 FR 42536 - Real-Time System Management Information Program

    Science.gov (United States)

    2011-07-19

    ... Federal Highway Administration 23 CFR Part 511 RIN 2125-AF19 Real-Time System Management Information... additional comments relating to the costs and benefits of the Real-Time System Management Information Program... System Management Information Program on November 8, 2010, at 75 FR 68418. The final rule document...

  11. 75 FR 68418 - Real-Time System Management Information Program

    Science.gov (United States)

    2010-11-08

    ... Federal Highway Administration 23 CFR Part 511 RIN 2125-AF19 Real-Time System Management Information... System Management Information Program that provides, in all States, the capability to monitor, in real... traveler information. The purposes of the Real-Time System Management Information Program are to:...

  12. Real-Time PCR for Universal Phytoplasma Detection and Quantification

    DEFF Research Database (Denmark)

    Christensen, Nynne Meyn; Nyskjold, Henriette; Nicolaisen, Mogens

    2013-01-01

    Currently, the most efficient detection and precise quantification of phytoplasmas is by real-time PCR. Compared to nested PCR, this method is less sensitive to contamination and is less work intensive. Therefore, a universal real-time PCR method will be valuable in screening programs and in other...

  13. Distributed, Embedded and Real-time Java Systems

    CERN Document Server

    Wellings, Andy

    2012-01-01

    Research on real-time Java technology has been prolific over the past decade, leading to a large number of corresponding hardware and software solutions, and frameworks for distributed and embedded real-time Java systems.  This book is aimed primarily at researchers in real-time embedded systems, particularly those who wish to understand the current state of the art in using Java in this domain.  Much of the work in real-time distributed, embedded and real-time Java has focused on the Real-time Specification for Java (RTSJ) as the underlying base technology, and consequently many of the Chapters in this book address issues with, or solve problems using, this framework. Describes innovative techniques in: scheduling, memory management, quality of service and communication systems supporting real-time Java applications; Includes coverage of multiprocessor embedded systems and parallel programming; Discusses state-of-the-art resource management for embedded systems, including Java’s real-time garbage collect...

  14. RT-PLG: Real Time Process Log Generator

    DEFF Research Database (Denmark)

    Yahya, Bernardo Nugroho; Khosiawan, Yohanes; Choi, Woosik;

    2016-01-01

    . This paper aims to develop a real time process log generator for the usage of streaming process mining tool. The real time process log generator (RT-PLG) is constructed in an independent tool. Afterward, the RT-PLG is utilized to generate a synthetic log for streaming process mining. The tool has been...... evaluated using an existing simulation model....

  15. Intelligent, Real-Time Problem Solving. Phase 3

    Science.gov (United States)

    1992-04-30

    approach, and to develop a heuristic algorithm for design-to-time real-time scheduling. E .velopes. During Phase III we extended our work on the real...the anytime algorithm approach, and presents a heuristic algorithm for design-to-time real-time scheduling. The methodology is known as design-to

  16. Real time ray tracing of skeletal implicit surfaces

    DEFF Research Database (Denmark)

    Rouiller, Olivier; Bærentzen, Jakob Andreas

    Modeling and rendering in real time is usually done via rasterization of polygonal meshes. We present a method to model with skeletal implicit surfaces and an algorithm to ray trace these surfaces in real time in the GPU. Our skeletal representation of the surfaces allows to create smooth models...

  17. Compilation and synthesis for real-time embedded controllers

    DEFF Research Database (Denmark)

    Fränzle, Martin; Müller-Olm, Markus

    1999-01-01

    This article provides an overview over two constructive approaches to provably correct hard real-time code generation where hard real-time code is generated from abstract requirements rather than verified against the timing requirements a posteriori. The first, more pragmatic approach is concerne...

  18. A Real-time Data Model Based on Temporal Data

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xiao-fang; LIU Yun-sheng

    2006-01-01

    Real-time database systems contain not only transaction timing constraints, but also data timing constraints. This paper discusses the temporal characteristics of data in real-time databases and offers a definition of absolute and relative temporal consistency. In real-time database systems, it is often the case that the policies of transaction schedules only consider the deadline of real-time transactions, making it insufficient to ensure temporal correctness of transactions. A policy is given by considering both the deadlines of transactions and the "data deadline" to schedule real-time transactions. A real-time relational data model and a real-time relational algebra based on the characteristics of temporal data are also proposed. In this model, the temporal data has not only corresponding values, but also validity intervals corresponding to the data values. At the same time, this model is able to keep historical data values. When validity interval of a relation is[NOW,NOW], real-time relational algebra will transform to traditional relational algebra.

  19. Real-Time Adaptation of Influence Strategies in Online Selling

    NARCIS (Netherlands)

    Kaptein, M.C.; Parvinen, P.

    2014-01-01

    Real-time adjustments in online selling are becoming increasingly common. In this paper we describe a novel method of real-time adaptation, and introduce influence strategies as a useful level of analysis for personalization of online selling. The proposed method incorporates three perspectives on r

  20. Recent achievements in real-time computational seismology in Taiwan

    Science.gov (United States)

    Lee, S.; Liang, W.; Huang, B.

    2012-12-01

    Real-time computational seismology is currently possible to be achieved which needs highly connection between seismic database and high performance computing. We have developed a real-time moment tensor monitoring system (RMT) by using continuous BATS records and moment tensor inversion (CMT) technique. The real-time online earthquake simulation service is also ready to open for researchers and public earthquake science education (ROS). Combine RMT with ROS, the earthquake report based on computational seismology can provide within 5 minutes after an earthquake occurred (RMT obtains point source information < 120 sec; ROS completes a 3D simulation < 3 minutes). All of these computational results are posted on the internet in real-time now. For more information, welcome to visit real-time computational seismology earthquake report webpage (RCS).

  1. Real-time feedback from iterative electronic structure calculations

    CERN Document Server

    Vaucher, Alain C; Reiher, Markus

    2015-01-01

    Real-time feedback from iterative electronic structure calculations requires to mediate between the inherently unpredictable execution times of the iterative algorithm employed and the necessity to provide data in fixed and short time intervals for real-time rendering. We introduce the concept of a mediator as a component able to deal with infrequent and unpredictable reference data to generate reliable feedback. In the context of real-time quantum chemistry, the mediator takes the form of a surrogate potential that has the same local shape as the first-principles potential and can be evaluated efficiently to deliver atomic forces as real-time feedback. The surrogate potential is updated continuously by electronic structure calculations and guarantees to provide a reliable response to the operator for any molecular structure. To demonstrate the application of iterative electronic structure methods in real-time reactivity exploration, we implement self-consistent semi-empirical methods as the data source and a...

  2. Ambiguity and Concepts in Real Time Online Internet Traffic Classification

    Directory of Open Access Journals (Sweden)

    Hamza Awad Hamza Ibrahim

    2014-03-01

    Full Text Available Internet traffic classification gained significant attention in the last few years. Identifying the Internet applications in the real time is one of the most significant challenges in network traffic classification. Most of the proposed classification methods are limited to offline classification and cannot support online classification. This paper aims to highlight the ambiguity in the definition of online classification. Therefore, some of the previous online classification works are discussed and analyzed. This analysing is to check how far the real time online classification was achieved. The results indicate that most of the previous works consider a real Internet traffic but did not consider a real time online classification. In addition, the paper provides a real time classifier which was proposed and used in [1] [2] [3], to show how to perform a real time online classification.

  3. Academic Training: Real Time Process Control - Lecture series

    CERN Multimedia

    Françoise Benz

    2004-01-01

    ACADEMIC TRAINING LECTURE REGULAR PROGRAMME 7, 8 and 9 June From 11:00 hrs to 12:00 hrs - Main Auditorium bldg. 500 Real Time Process Control T. Riesco / CERN-TS What exactly is meant by Real-time? There are several definitions of real-time, most of them contradictory. Unfortunately the topic is controversial, and there does not seem to be 100% agreement over the terminology. Real-time applications are becoming increasingly important in our daily lives and can be found in diverse environments such as the automatic braking system on an automobile, a lottery ticket system, or robotic environmental samplers on a space station. These lectures will introduce concepts and theory like basic concepts timing constraints, task scheduling, periodic server mechanisms, hard and soft real-time.ENSEIGNEMENT ACADEMIQUE ACADEMIC TRAINING Françoise Benz 73127 academic.training@cern.ch

  4. Survey of real-time processing systems for big data

    DEFF Research Database (Denmark)

    Iftikhar, Nadeem

    2014-01-01

    In recent years, real-time processing and analytics systems for big data–in the context of Business Intelligence (BI)–have received a growing attention. The traditional BI platforms that perform regular updates on daily, weekly or monthly basis are no longer adequate to satisfy the fast......-changing business environments. However, due to the nature of big data, it has become a challenge to achieve the real-time capability using the traditional technologies. The recent distributed computing technology, MapReduce, provides off-the-shelf high scalability that can significantly shorten the processing time...... for big data; Its open-source implementation such as Hadoop has become the de-facto standard for processing big data, however, Hadoop has the limitation of supporting real-time updates. The improvements in Hadoop for the real-time capability, and the other alternative real-time frameworks have been...

  5. Hyd5 gene based analysis of cereals and malt for gushing-inducing Fusarium spp. by real-time LAMP using fluorescence and turbidity measurements.

    Science.gov (United States)

    Denschlag, Carla; Vogel, Rudi F; Niessen, Ludwig

    2013-04-01

    The surface active class 2 hydrophobin Hyd5p (GenBank accession number DQ449530) has been identified as a causative agent for over foaming (gushing) of beer. In order to estimate the potential of brewing cereals and malt to induce gushing in beer we used a previously described set of primers to amplify a partial sequence of the hyd5 gene in Fusarium culmorum and closely related species in a real-time loop-mediated isothermal amplification assay. Real-time LAMP was optimized and performed on two different platforms using a turbidimeter and a fluorescence reader to monitor the reaction on line. Serial dilutions of purified target DNA were used to set up a calibration curve for quantitative estimation of DNA concentrations. Analysis of model barley samples prepared by mixing infected with non-infected material in different ratios demonstrated a positive correlation between the real-time LAMP results and respective infection levels. In order to investigate the performance of the newly established methods, samples of barley were analyzed for their gushing potential using the Hyd5 real-time LAMP assay and a reference in vitro test for gushing prediction (Modified Carlsberg Test, MCT) in parallel experiments. Results showed that the real-time LAMP assay was in accordance with the reference test in 50% of cases with both platforms used. It predicted a lower number of gushing-positive samples as compared to the current reference test.

  6. Allelopathic effects of barley straw on germination and seedling growth of corn, sugar beet and sunflower

    Directory of Open Access Journals (Sweden)

    mohamad taghi naseri poor yazdi

    2009-06-01

    Full Text Available Allelopathic effects of barley straw and root on germination and growth of maize, sugar beet, and sunflower were investigated under glasshouse and laboratory experiments in Faculty of Agriculture, Ferdowsi University of Mashhad in 2006. The glasshouse experiment was designed based on randomized complete block design with three replications, treatments included: 0, 200, 400, 600 g/m² of grounded barley straw and also 0 and 50 g/m2 barley root. A laboratory experiment was carried out in order to study the effect of different concentrations of barley water extracts on germination and seedling characteristics of corn, sugar beet and sunflower. Treatments in laboratory trial included 0, 33, 50 and 100 percent of barley extracts. Results showed that leaf area of corn was significantly affected by barley straw treatments. Shoot dry matter and seed weight per plant in corn , leaf and tuber weight in sugar beet and leaf , stem weights , plant per plant in corn , leaf and tuber weight in sugar beet and leaf, stem weights, plant height, head diameter, head weight and seed weight in sunflower were significantly higher in treatment of 50g/m² barley roots. Crop seed germination decreased with increasing the amount of barley straw. The best germination response to barley extract was observed in corn. Maize radicle weight was significantly decreased with increasing concentration of barley water extract.

  7. Scheduling Non-stationary Bursts of Real-time and Non-real-time Traffic in ATM Networks

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The problem of scheduling real-time and non-real-time traffic in an ATM switch multiplexor when bursts of either type of traffic occur is studied. The scheduling algorithms studied are: Queue Length Threshold (QLT) and Minimum Laxity Threshold (MLT). Analytic results based on Markov Chains are used. In addition the results are compared with an optimal (but impractical) scheduling determined via dynamic programming. Dynamic programming is used in this paper to show that MLT gives a near optimal performance trade-off between real-time and non-real-time traffic for constant arrival rates. The trade-off QLT allows is not close to optimal. For non-real-time bursts MLT still gives a close to optimal trade-off. For real-time bursts the trade-off MLT allows between real-time and non-real-time traffic is not as close to optimal, but even where the MLT trade-off is not near optimal, the QLT trade-off is much worse than the MLT trade-off.

  8. Barley Stripe Mosaic Virus and the Frequency of Triploids and Aneuploids in Barley

    DEFF Research Database (Denmark)

    Sandfær, J.

    1973-01-01

    BSMV infection caused a pronounced increase in the frequency of triploid and aneuploid seeds in eleven barley varieties, but with considerable variation in frequency among varieties. In some of the varieties triploids exceeded three per cent. In virus-free material a few triploids were found in m...

  9. [Rapid detection of rotavirus in water samples using immunomagnetic separation combined with real time PCR].

    Science.gov (United States)

    Yang, Wan; He, Miao; Li, Dan; Shi, Han-Chang; Liu, Li

    2009-05-15

    A quantitative and rapid detection method for rotavirus in water samples was developed, by using immunomagnetic separation combined with reverse transcription and real time polymerase chain reaction (IMS-RT-real time PCR). Magnetic beads coated with antibodies directed against group A rotavirus were used to capture and purify the virus in water samples. The experimental results showed that IMS was optimized when 1 mL samples were supplemented with 10 microL of immunomagnetic beads, 2.5 microL of Tween 20 and incubated for 2 h. The IMS method was employed in the detection of rotavirus in seeded virus eluant such as 3% beef extract successfully and thus manifested its compatibility with established virus concentration methods. The IMS-RT-real time PCR method could yield quantitative results within about 5 h with a detection limit at 1 x 10(4) copies/mL (equivalent to 3-4 PFU/mL). The method exhibited a high level correlation (R2 = 0.9816) with cell culture assay, indicating that it could perform as well as cell culture assay does in infection tests. And the method functioned satisfactorily in seeded concentrate of secondary waste water treatment plant effluent, reclaimed water, surface water and tap water.

  10. Real-time Fluorescence PCR Method for Detection of Burkholderia glumae from Rice

    Institute of Scientific and Technical Information of China (English)

    FANG Yuan; XU Li-hui; TIAN Wen-xiao; HUAI Yan; YU Shan-hong; LOU Miao-miao; XIE Guan-lin

    2009-01-01

    Burkholderia glumae causing seedling rot and grain rot of rice was listed as a plant quarantine disease of China in 2007. It's quite necessary to set up effective detection methods for the pathogen to manage further dispersal of this disease. The present study combined the real-time PCR method with classical PCR to increase the detecting efficiency, and to develop an accurate, rapid and sensitive method to detect the pathogen in the seed quarantine for effective management of the disease. The results showed that all the tested strains of B. glumae produced about 139 bp specific fragments by the real-time PCR and the general PCR methods, while others showed negative PCR result. The bacteria could be detected at the concentrations of 1×104 CFU/mL by general PCR method and at the concentrations below 100 CFU/mL by real-time fluorescence PCR method. B. glumae could be detected when the inoculated and healthy seeds were mixed with a proportion of 1:100.

  11. Space Shuttle Main Engine real time stability analysis

    Science.gov (United States)

    Kuo, F. Y.

    1993-01-01

    The Space Shuttle Main Engine (SSME) is a reusable, high performance, liquid rocket engine with variable thrust. The engine control system continuously monitors the engine parameters and issues propellant valve control signals in accordance with the thrust and mixture ratio commands. A real time engine simulation lab was installed at MSFC to verify flight software and to perform engine dynamic analysis. A real time engine model was developed on the AD100 computer system. This model provides sufficient fidelity on the dynamics of major engine components and yet simplified enough to be executed in real time. The hardware-in-the-loop type simulation and analysis becomes necessary as NASA is continuously improving the SSME technology, some with significant changes in the dynamics of the engine. The many issues of interfaces between new components and the engine can be better understood and be resolved prior to the firing of the engine. In this paper, the SSME real time simulation Lab at the MSFC, the SSME real time model, SSME engine and control system stability analysis, both in real time and non-real time is presented.

  12. Reviewing real-time performance of nuclear reactor safety systems

    Energy Technology Data Exchange (ETDEWEB)

    Preckshot, G.G. [Lawrence Livermore National Lab., CA (United States)

    1993-08-01

    The purpose of this paper is to recommend regulatory guidance for reviewers examining real-time performance of computer-based safety systems used in nuclear power plants. Three areas of guidance are covered in this report. The first area covers how to determine if, when, and what prototypes should be required of developers to make a convincing demonstration that specific problems have been solved or that performance goals have been met. The second area has recommendations for timing analyses that will prove that the real-time system will meet its safety-imposed deadlines. The third area has description of means for assessing expected or actual real-time performance before, during, and after development is completed. To ensure that the delivered real-time software product meets performance goals, the paper recommends certain types of code-execution and communications scheduling. Technical background is provided in the appendix on methods of timing analysis, scheduling real-time computations, prototyping, real-time software development approaches, modeling and measurement, and real-time operating systems.

  13. Real-time embedded systems design principles and engineering practices

    CERN Document Server

    Fan, Xiaocong

    2015-01-01

    This book integrates new ideas and topics from real time systems, embedded systems, and software engineering to give a complete picture of the whole process of developing software for real-time embedded applications. You will not only gain a thorough understanding of concepts related to microprocessors, interrupts, and system boot process, appreciating the importance of real-time modeling and scheduling, but you will also learn software engineering practices such as model documentation, model analysis, design patterns, and standard conformance. This book is split into four parts to help you

  14. Towards Real Time Simulation of Ship-Ship Interaction

    DEFF Research Database (Denmark)

    Lindberg, Ole; Bingham, Harry B.; Engsig-Karup, Allan Peter

    2012-01-01

    We present recent and preliminary work directed towards the development of a simplified, physics-based model for improved simulation of ship-ship interaction that can be used for both analysis and real-time computing (i.e. with real-time constraints due to visualization). The goal is to implement...... accurate (realistic) and much faster ship-wave and ship-ship simulations than are currently possible. The coupling of simulation with visualization should improve the visual experience such that it can be perceived as more realistic in training. Today the state-of-art in real-time ship-ship interaction...

  15. The Durham adaptive optics real-time controller

    CERN Document Server

    Basden, Alastair; Myers, Richard; Younger, Eddy

    2010-01-01

    The Durham adaptive optics real-time controller was initially a proof of concept design for a generic adaptive optics control system. It has since been developed into a modern and powerful CPU based real-time control system, capable of using hardware acceleration (including FPGAs and GPUs), based primarily around commercial off the shelf hardware. It is powerful enough to be used as the real-time controller for all currently planned 8~m class telescope adaptive optics systems. Here we give details of this controller and the concepts behind it, and report on performance including latency and jitter, which is less than 10~$\\mu$s for small adaptive optics systems.

  16. Real-Time Mass Passenger Transport Network Optimization Problems

    OpenAIRE

    2005-01-01

    The aim of Real-Time Mass Transport Vehicle Routing Problem (MTVRP) is to find a solution to route n vehicles in real time to pick up and deliver m passengers. This problem is described in the context of flexible large-scale mass transportation options that use new technologies for communication among passengers and vehicles. The solution of such a problem is relevant to future transportation options involving large scale real-time routing of shared-ride fleet transit vehicles. However, the g...

  17. Screening and reconstruction of real-time traffic data

    Institute of Scientific and Technical Information of China (English)

    裴玉龙; 马骥

    2003-01-01

    The quality of real-time traffic information is of the great importance, therefore the factors having effect on traffic characteristics are analyzed in general, and the necessities of real-time data processing are summarized. The identification and reconstruction of real-time traffic data are analyzed using Kalman filter equation and statistical approach. Four methods for ITS (Intelligent transportation system) detector data screening in traffic management systems are discussed in detail. Meanwhile traffic data examinations are discussed with solutions formulated through analysis, and recommendations are made for information collection and data management in future.

  18. A framework to model real-time databases

    CERN Document Server

    Idoudi, Nizar; Duvallet, Claude; Sadeg, Bruno; Bouaziz, Rafik; Gargouri, Faiez

    2010-01-01

    Real-time databases deal with time-constrained data and time-constrained transactions. The design of this kind of databases requires the introduction of new concepts to support both data structures and the dynamic behaviour of the database. In this paper, we give an overview about different aspects of real-time databases and we clarify requirements of their modelling. Then, we present a framework for real-time database design and describe its fundamental operations. A case study demonstrates the validity of the structural model and illustrates SQL queries and Java code generated from the classes of the model

  19. Real-Time Obstacle Detection Approach using Stereoscopic Images

    Directory of Open Access Journals (Sweden)

    Nadia Baha

    2014-02-01

    Full Text Available In this paper, we propose a new and simple approach to obstacle and free space detection in an indoor and outdoor environment in real-time using stereo vision as sensor. The real-time obstacle detection algorithm uses two dimensional disparity map to detect obstacles in the scene without constructing the ground plane. The proposed approach combines an accumulating and thresholding techniques to detect and cluster obstacle pixels into objects using a dense disparity map. The results from both analysis modules are combined to provide information of the free space. Experimental results are presented to show the effectiveness of the proposed method in real-time.

  20. Time-dependent viscometry study of endoglucanase action on xyloglucan: A real-time approach.

    Science.gov (United States)

    Spier, Vivian Cristina; Sierakowski, Maria Rita; Ibrahim, Amid Tony; Scholze Baum, Jéssica C; Silveira, Joana Lea M; de Freitas, Rilton Alves

    2015-11-01

    Hydrolysis of xyloglucan from Tamarindus indica and Hymenaea courbaril seeds with endoglucanase (EGII), which randomly breaks the (1→4)-linked β-glycosidic bonds of the polymer chain, was monitored in real time using time-dependent viscometry analysis (TDV). For both samples there was a decrease in the intrinsic viscosity ([η]), viscosity average molar mass (Mv), radius de gyration (Rg) and persistence length (Lp) immediately after the addition of the enzyme. It was observed the formation of oligosaccharides and oligomers composed of ∼2 units, up to 140min. Galactose-containing side chains two positions away from the non-substituted glucose, modulated the action of EGII, and the complete hydrolysis of the XG oligomers occurred after 24h. The results demonstrate for the first time the real-time degradation of xyloglucan as well the macromolecular and oligosaccharide composition during the EGII hydrolysis process.

  1. Novel Real-Time Flight Envelope Monitoring System Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The proposed innovation is an aircraft flight envelope monitoring system that will provide real-time in-cockpit estimations of aircraft flight envelope boundaries,...

  2. Study on the Bus Real-time Dispatching Model

    Institute of Scientific and Technical Information of China (English)

    YingZou; JianguoLi; JianhuaHuang; ZhenminTang

    2004-01-01

    The real-time dispatching model on bus system is studied in this article. Realtime dispatching can resume the planned schedule quickly, and then to ensure the reliability of the public transport service and to well raise the service quality.

  3. 3D Flash LIDAR Real-Time Embedded Processing Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Advanced Scientific Concepts, Inc. (ASC) is a small business, which has developed a compact, eye-safe 3D Flash LIDARTM Camera (FLC) well suited for real-time...

  4. Real Time Control Software for Electromagnetic Formation Flight Project

    Data.gov (United States)

    National Aeronautics and Space Administration — We propose the development of a maintainable and evolvable real-time control software system for Electromagnetic Formation Flight (EMFF). EMFF systems use...

  5. RadNet Real-Time Monitoring Spectrometry Data Inventory

    Data.gov (United States)

    U.S. Environmental Protection Agency — The RadNet Real-Time Monitoring Spectrometry Data Inventory contains measured data used to identify and measure specific radioactive materials in the atmosphere at...

  6. RadNet-Air Near Real Time Data

    Data.gov (United States)

    U.S. Environmental Protection Agency — RadNet-Air is a national network of air monitoring stations that regularly collect air samples for near real time analysis of radioactivity. The data is transmitted...

  7. Real Time Scheduling Services for Distributed RT-CORBA Applications

    Directory of Open Access Journals (Sweden)

    Bineta Tresa Mathew

    2012-12-01

    Full Text Available Distributed computing environment is flexible to control in complex embedded systems and their software components gain complexity when these systems are equipped with many microcontrollers and software object which covers diverse platforms, this system is called as DRE system. These DRE systems need new inter-object communication solution thus QoS-enabled middleware services and mechanisms have begun to emerge. Real-time application domain benefit from flexible and open distributed architectures, such as those defined by the CORBA specification. CORBA is well-suited to conventional request/response applications, but not suited to real-time applications due to the lack of QoS features and performance optimizations. The paper shows the design and implementation of the high performance scheduling technique for the real time applications domain with CORBA systems. Four different algorithms are compared by using attributes of real time tasks constraints based on CORBA specification such as RMS, MLF, MUF and EDF.

  8. Real-time Position Control of Concentric Tube Robots.

    Science.gov (United States)

    Dupont, Pierre E; Lock, Jesse; Itkowitz, Brandon

    2010-05-03

    A novel approach to constructing robots is based on concentrically combining pre-curved elastic tubes. By rotating and extending the tubes with respect to each other, their curvatures interact elastically to position and orient the robot's tip, as well as to control the robot's shape along its length. Since these robots form slender curves, they are well suited for minimally invasive medical procedures. A substantial challenge to their practical use is the real-time solution of their kinematics that are described by differential equations with split boundary equations. This paper proposes a numerically efficient approach to real-time position control. It is shown that the forward kinematics are smooth functions that can be pre-computed and accurately approximated using Fourier series. The inverse kinematics can be solved in real time using root finding applied to the functional approximation. Experimental demonstration of real-time position control using this approach is also described.

  9. Real-Time Smart Tools for Processing Spectroscopy Data Project

    Data.gov (United States)

    National Aeronautics and Space Administration — We propose novel and real-time smart software tools to process spectroscopy data. Material abundance or compositional maps will be generated for rover guidance,...

  10. NOAA Ship Rainier Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Rainier Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System (SAMOS)...

  11. Novel Real-Time Flight Envelope Monitoring System Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The proposed innovation is an aircraft flight envelope monitoring system that will provide real-time in-cockpit estimations of aircraft flight envelope boundaries....

  12. Analyzing Real-Time Systems: Theory and Tools

    DEFF Research Database (Denmark)

    Hune, Thomas Seidelin

    actions take place, but also the timing of the actions. The formal reasoning presented here is based on (extensions of) the model of timed automata and tools supporting this model, mainly UPPAAL. Real-time systems are often part of safety critical systems e.g. control systems for planes, trains......The main topic of this dissertation is the development and use of methods for formal reasoning about the correctness of real-time systems, in particular methods and tools to handle new classes of problems. In real-time systems the correctness of the system does not only depend on the order in which......, or factories, though also everyday electronics as audio/video equipment and (mobile) phones are considered real-time systems. Often these systems are concurrent systems with a number of components interacting, and reasoning about such systems is notoriously difficult. However, since most of the systems...

  13. NOAA Ship Oregon II Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Oregon II Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System (SAMOS)...

  14. NOAA Ship Ronald Brown Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Ronald Brown Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System (SAMOS)...

  15. NOAA Ship Fairweather Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Fairweather Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System (SAMOS)...

  16. NOAA Ship Nancy Foster Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Nancy Foster Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System (SAMOS)...

  17. NOAA Ship Gordon Gunter Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Gordon Gunter Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System (SAMOS)...

  18. NOAA Ship Okeanos Explorer Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Okeanos Explorer Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System...

  19. A New Scheduling Algorithms For Real Time Tasks

    CERN Document Server

    Yaashuwanth, C

    2009-01-01

    The main objective of this paper is to develop the two different ways in which round robin architecture is modified and made suitable to be implemented in real time and embedded systems. The scheduling algorithm plays a significant role in the design of real time embedded systems. Simple round robin architecture is not efficient to be implemented in embedded systems because of higher context switch rate, larger waiting time and larger response time. Missing of deadlines will degrade the system performance in soft real time systems. The main objective of this paper is to develop the scheduling algorithm which removes the drawbacks in simple round robin architecture. A comparison with round robin architecture to the proposed architectures has been made. It is observed that the proposed architectures solves the problems encountered in round robin architecture in soft real time by decreasing the number of context switches waiting time and response time thereby increasing the system throughput.

  20. Scheduling Algorithms Based on Weakly Hard Real-Time Constraints

    Institute of Scientific and Technical Information of China (English)

    TU Gang (涂刚); YANG FuMin (阳富民); LU YanSheng (卢炎生)

    2003-01-01

    The problem of scheduling weakly hard real-time tasks is addressed in this paper.The paper first analyzes the characters ofμ-pattern and weakly hard real-time constraints, then,presents two scheduling algorithms, Meet Any Algorithm and Meet Row Algorithm, for weakly hard real-time systems. Different from traditional algorithms used to guarantee deadlines, Meet Any Algorithm and Meet Row Algorithm can guarantee both deadlines and constraints. Meet Any Algorithm and Meet Row Algorithm try to find out the probabilities of tasks breaking constraints and increase task's priority in advance, but not till the last moment. Simulation results show that these two algorithms are better than other scheduling algorithms dealing with constraints and can largely decrease worst-case computation time of real-time tasks.

  1. UML statechart based rigorous modeling of real-time system

    Institute of Scientific and Technical Information of China (English)

    LAI Ming-zhi; YOU Jin-yuan

    2005-01-01

    Rigorous modeling could ensure correctness and could verify a reduced cost in embedded real-time system development for models. Software methods are needed for rigorous modeling of embedded real-time systems. PVS is a formal method with precise syntax and semantics defined. System modeled by PVS specification could be verified by tools. Combining the widely used UML with PVS, this paper provides a novel modeling and verification approach for embedded real-time systems. In this approach, we provide 1 ) a time-extended UML statechart for modeling dynamic behavior of an embedded real-time system; 2) an approach to capture timed automata based semantics from a timed statechart; and 3) an algorithm to generate a finite state model expressed in PVS specification for model checking. The benefits of our approach include flexibility and user friendliness in modeling, extendability in formalization and verification content, and better performance. Time constraints are modeled and verified and is a highlight of this paper.

  2. NOAA Ship Pisces Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Pisces Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System (SAMOS)...

  3. Real-time water quality monitoring and providing water quality ...

    Science.gov (United States)

    EPA and the U.S. Geological Survey (USGS) have initiated the “Village Blue” research project to provide real-time water quality monitoring data to the Baltimore community and increase public awareness about local water quality in Baltimore Harbor and the Chesapeake Bay. The Village Blue demonstration project complements work that a number of state and local organizations are doing to make Baltimore Harbor “swimmable and fishable” 2 by 2020. Village Blue is designed to build upon EPA’s “Village Green” project which provides real-time air quality information to communities in six locations across the country. The presentation, “Real-time water quality monitoring and providing water quality information to the Baltimore Community”, summarizes the Village Blue real-time water quality monitoring project being developed for the Baltimore Harbor.

  4. 3D Flash LIDAR Real-Time Embedded Processing Project

    Data.gov (United States)

    National Aeronautics and Space Administration — 3D Flash LIDAR (3DFL) is ideal for determining real-time spacecraft trajectory, speed and orientation to the planet surface, as well as evaluating potential hazards...

  5. NOAA Ship Oscar Dyson Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Oscar Dyson Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System (SAMOS)...

  6. Hard-real-time resource management for autonomous spacecraft

    Science.gov (United States)

    Gat, E.

    2000-01-01

    This paper describes tickets, a computational mechanism for hard-real-time autonomous resource management. Autonomous spacecraftcontrol can be considered abstractly as a computational process whose outputs are spacecraft commands.

  7. Decision graphs: a tool for developing real-time software

    Energy Technology Data Exchange (ETDEWEB)

    Kozubal, A.J.

    1981-01-01

    The use of decision graphs in the preparation of, in particular, real-time software is briefly described. The usefulness of decision graphs in software design, testing, and maintenance is pointed out. 2 figures. (RWR)

  8. The Effects of Real-Time Interactive Multimedia Teleradiology System.

    Science.gov (United States)

    Al-Safadi, Lilac

    2016-01-01

    This study describes the design of a real-time interactive multimedia teleradiology system and assesses how the system is used by referring physicians in point-of-care situations and supports or hinders aspects of physician-radiologist interaction. We developed a real-time multimedia teleradiology management system that automates the transfer of images and radiologists' reports and surveyed physicians to triangulate the findings and to verify the realism and results of the experiment. The web-based survey was delivered to 150 physicians from a range of specialties. The survey was completed by 72% of physicians. Data showed a correlation between rich interactivity, satisfaction, and effectiveness. The results of our experiments suggest that real-time multimedia teleradiology systems are valued by referring physicians and may have the potential for enhancing their practice and improving patient care and highlight the critical role of multimedia technologies to provide real-time multimode interactivity in current medical care.

  9. Optical Real-Time Space Radiation Monitor Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Real-time dosimetry is needed to provide immediate feedback, so astronauts can minimize their exposure to ionizing radiation during periods of high solar activity....

  10. Cluster Computing for Embedded/Real-Time Systems

    Science.gov (United States)

    Katz, D.; Kepner, J.

    1999-01-01

    Embedded and real-time systems, like other computing systems, seek to maximize computing power for a given price, and thus can significantly benefit from the advancing capabilities of cluster computing.

  11. Specification and compilation of real-time stream processing applications

    NARCIS (Netherlands)

    Geuns, Stephanus Joannes

    2015-01-01

    This thesis is concerned with the specification, compilation and corresponding temporal analysis of real-time stream processing applications that are executed on embedded multiprocessor systems. An example of such applications are software defined radio applications. These applications typically hav

  12. Real-Time Multi-Directional Equipment Site

    Data.gov (United States)

    Federal Laboratory Consortium — As part of the George E. Brown, Jr. Network for Earthquake Engineering Simulation (NEES) Program, Lehigh University has established the Real-Time Multi-Directional...

  13. Transformer real-time reliability model based on operating conditions

    Institute of Scientific and Technical Information of China (English)

    HE Jian; CHENG Lin; SUN Yuan-zhang

    2007-01-01

    Operational reliability evaluation theory reflects real-time reliability level of power system. The component failure rate varies with operating conditions. The impact of real-time operating conditions such as ambient temperature and transformer MVA (megavolt-ampere) loading on transformer insulation life is studied in this paper. The formula of transformer failure rate based on the winding hottest-spot temperature (HST) is given. Thus the real-time reliability model of transformer based on operating conditions is presented. The work is illustrated using the 1979 IEEE Reliability Test System. The changes of operating conditions are simulated by using hourly load curve and temperature curve, so the curves of real-time reliability indices are obtained by using operational reliability evaluation.

  14. Axo: Masking Delay Faults in Real-Time Control Systems

    OpenAIRE

    Maaz, Mashood Mohiuddin; Saab, Wajeb; Bliudze, Simon; Le Boudec, Jean-Yves

    2016-01-01

    We consider real-time control systems that consist of a controller that computes and sends setpoints to be implemented in physical processes through process agents. We focus on systems that use commercial off-the-shelf hardware and software components. Setpoints of these systems have strict real-time constraints: Implementing a setpoint after its deadline, or not receiving setpoints within a deadline, can cause failure. In this paper, we address delay faults: faults that cause setpoints to vi...

  15. Streaming Surface Reconstruction from Real Time 3D Measurements

    OpenAIRE

    Bodenmüller, Tim

    2009-01-01

    In this thesis, a robust method for fast surface reconstruction from real time 3D point streams is presented. It is designed for the integration in a fast visual feedback system that supports a user while manually 3D scanning objects. The method iteratively generates a dense and homogeneous triangular mesh by inserting sample points from the real time data stream and refining the surface model locally. A spatial data structure ensures a fast access to growing point sets and continuously updat...

  16. Implementation of real-time duplex synthetic aperture ultrasonography

    DEFF Research Database (Denmark)

    Hemmsen, Martin Christian; Larsen, Lee; Kjeldsen, Thomas;

    2015-01-01

    This paper presents a real-time duplex synthetic aperture imaging system, implemented on a commercially available tablet. This includes real-time wireless reception of ultrasound signals and GPU processing for B-mode and Color Flow Imaging (CFM). The objective of the work is to investigate the im...... and that the required bandwidth between the probe and processing unit is within the current Wi-Fi standards....

  17. Design a Fault Tolerance for Real Time Distributed System

    OpenAIRE

    Ban M. Khammas

    2012-01-01

    This paper designed a fault tolerance for soft real time distributed system (FTRTDS). This system is designed to be independently on specific mechanisms and facilities of the underlying real time distributed system. It is designed to be distributed on all the computers in the distributed system and controlled by a central unit.Besides gathering information about a target program spontaneously, it provides information about the target operating system and the target hardware in order to diagno...

  18. Real-Time Connect 4 Game Using Artificial Intelligence

    Directory of Open Access Journals (Sweden)

    Ahmad M. Sarhan

    2009-01-01

    Full Text Available Problem statement: The study presented a design that converted connect 4 game into a real-time game by incorporating time restraints. Approach: The design used Artificial Intelligence (AI in implementing the connect 4 game. The AI for this game was based on influence mapping. Results: A waterfall-based AI software was developed for a Connect 4 game. Conclusion: A real time connect 4 game was successfully designed and implanted with GUI using C++ programming language.

  19. Near real-time stereo matching using geodesic diffusion.

    Science.gov (United States)

    De-Maeztu, Leonardo; Villanueva, Arantxa; Cabeza, Rafael

    2012-02-01

    Adaptive-weight algorithms currently represent the state of the art in local stereo matching. However, due to their computational requirements, these types of solutions are not suitable for real-time implementation. Here, we present a novel aggregation method inspired by the anisotropic diffusion technique used in image filtering. The proposed aggregation algorithm produces results similar to adaptive-weight solutions while reducing the computational requirements. Moreover, near real-time performance is demonstrated with a GPU implementation of the algorithm.

  20. Tactical Atmospheric Modeling System-Real Time (TAMS-RT)

    Science.gov (United States)

    2016-06-07

    mesoscale model analysis and forecast fields as inputs. OBJECTIVES Support the NRL Tactical Atmospheric Modeling System- Real Time (TAMS-RT) installed in...SEP 1999 2. REPORT TYPE 3. DATES COVERED 00-00-1999 to 00-00-1999 4. TITLE AND SUBTITLE Tactical Atmospheric Modeling System- Real Time (TAMS...unclassified c. THIS PAGE unclassified Standard Form 298 (Rev. 8-98) Prescribed by ANSI Std Z39-18 1 Tactical Atmospheric Modeling System- Real

  1. THE DLSTRIBOTED MCU MODEL FOR SYNCHRONOUS REAL-TIME TELETEACHING

    Institute of Scientific and Technical Information of China (English)

    刘均; 李人厚; 郑庆华

    2004-01-01

    Aiming at issues on multimedia communication in synchronous real-time teleteaching (SRT) systems over IP network, a 4-tuple structural mode of multimedia communication is proposed in the paper, and an SRT-oriented distributed MCU model is built according to the mode. Moreover, the mechanism of multicast communication across subnets is discussed. The distributed MCU model has been applied successfully in our interactive synchronous real-time telesteaching system RealClass and has shown good extendibility in operation.

  2. Search-Space Characterization for Real-time Heuristic Search

    OpenAIRE

    Huntley, Daniel; Bulitko, Vadim

    2013-01-01

    Recent real-time heuristic search algorithms have demonstrated outstanding performance in video-game pathfinding. However, their applications have been thus far limited to that domain. We proceed with the aim of facilitating wider applications of real-time search by fostering a greater understanding of the performance of recent algorithms. We first introduce eight algorithm-independent complexity measures for search spaces and correlate their values with algorithm performance. The complexity ...

  3. A real time method for surface cleanliness measurement

    OpenAIRE

    Bilmes, Gabriel Mario; Orzi, Daniel Jesús Omar; Martínez , Oscar E.; Lencina, Alberto

    2005-01-01

    The measurement of surface cleanliness is a signifi cant problem in many industrial and technological processes. Existing methods are based on laboratory procedures, that are not performed in real time, can not be automated, and usually are restricted to a small portion of the sample. In this study we describe a new method for real time measurement of the amount of surface dirt or contamination deposited on a surface. It relies on the ablation of the surface dirt film by means of a ...

  4. Multi-Threat Real-Time Separating System

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    This paper describes a multi-threat real-time separating system for broadband anti-radiation missile seeker.It presents a method, with a dual-port memory as comparer, to perform PF and PW hardware real-time separation and to determine the time-of-arrival (TOA) by use of sequential difference histogram (SDIF). The method has been applied to practice, which has achieved good results.

  5. Real Time Collision Detection Using Depth Texturing Spheres

    Institute of Scientific and Technical Information of China (English)

    WANG Ji; ZHAI Zhengjun; CAI Xiaobin

    2006-01-01

    In this paper, we present a novel collision detection algorithm to real time detect the collisions of objects. We generate sphere textures of objects, and use programmable graphics hardware to mapping texture and check the depth of different objects to detect the collision. We have implemented the algorithm and compared it with CULLIDE. The result shows that our algorithm is more effective than CULLIDE and has fixed executive time to suit for real-time applications.

  6. Collaborative Real-time Digital Reference Services in Academic Libraries

    Directory of Open Access Journals (Sweden)

    Shiao-Feng Su

    2004-03-01

    Full Text Available The real-time digital reference service is valued by most academic librarians in United States. To make the collaborative real-time digital reference service viable in academic library service, the libraries need to understand the information seeking behavior of potential users and to train the staff well. The participating libraries also need to coordinate the following issues in advance: quality control, question routing, network standards, cultural and political sensitivities, communication, copyright, and marketing. [Article content in Chinese

  7. Real-time radiography at the NECTAR facility

    Energy Technology Data Exchange (ETDEWEB)

    Buecherl, T., E-mail: thomas.buecherl@radiochemie.de [Lehrstuhl fuer Radiochemie (RCM), Technische Universitaet Muenchen (TUM), Walther-Meissner-Str. 3, 85748 Garching (Germany); Lierse von Gostomski, Ch. [Lehrstuhl fuer Radiochemie (RCM), Technische Universitaet Muenchen (TUM), Walther-Meissner-Str. 3, 85748 Garching (Germany)

    2011-09-21

    A feasibility study has shown that real-time radiography using fission neutrons is possible at the NECTAR facility, when using an improved detection system for fast variations (Buecherl et al., 2009 ). Continuing this study, real-time measurements of slowly varying processes like the water uptake in medium sized trunks (diameter about 12 cm) and of slow periodic processes (e.g. a slowly rotating iron disk) are investigated successfully using the existing detection system.

  8. A Remote Real-Time Monitoring System for Power Quality

    Institute of Scientific and Technical Information of China (English)

    黄治清; 贺建闽

    2003-01-01

    An introduction is made to the composition, design method and engineering application of a remote real-time monitoring system of power quality in substations based on internet. With virtual instrument and network technique adopted, this system is characterized by good real-time property, high reliability, plentiful functions, and so on. It also can be used to monitor the load of a substation, such as electric locomotives.

  9. The Dynamic Checking of Complex Real Time System

    Institute of Scientific and Technical Information of China (English)

    YU Chao; HUANG Benwen; WU Guoqing

    2006-01-01

    The paper presents an dynamic execution model of complex real-time software based on requirement description model RTRSM, and then propose a checking method based on configuration covering and its corresponding algorithm. This checking method can check the execution situations between parallel elements in a dynamic execution step of real-time software systems. It also can check all the states and transitions which assure the completeness of checking. In the end, related theorem is proofed.

  10. Real time simulator with Ti floating point digital signal processor

    Energy Technology Data Exchange (ETDEWEB)

    Razazian, K.; Bobis, J.P.; Dieckman, S.L.; Raptis, A.C.

    1994-08-01

    This paper describes the design and operation of a Real Time Simulator using Texas Instruments TMS320C30 digital signal processor. This system operates with two banks of memory which provide the input data to digital signal processor chip. This feature enables the TMS320C30 to be utilized in variety of applications for which external connections to acquire input data is not needed. In addition, some practical applications of this Real Time Simulator are discussed.

  11. Can Real-Time Data Also Be Climate Quality?

    Science.gov (United States)

    Brewer, M.; Wentz, F. J.

    2015-12-01

    GMI, AMSR-2 and WindSat herald a new era of highly accurate and timely microwave data products. Traditionally, there has been a large divide between real-time and re-analysis data products. What if these completely separate processing systems could be merged? Through advanced modeling and physically based algorithms, Remote Sensing Systems (RSS) has narrowed the gap between real-time and research-quality. Satellite microwave ocean products have proven useful for a wide array of timely Earth science applications. Through cloud SST capabilities have enormously benefited tropical cyclone forecasting and day to day fisheries management, to name a few. Oceanic wind vectors enhance operational safety of shipping and recreational boating. Atmospheric rivers are of import to many human endeavors, as are cloud cover and knowledge of precipitation events. Some activities benefit from both climate and real-time operational data used in conjunction. RSS has been consistently improving microwave Earth Science Data Records (ESDRs) for several decades, while making near real-time data publicly available for semi-operational use. These data streams have often been produced in 2 stages: near real-time, followed by research quality final files. Over the years, we have seen this time delay shrink from months or weeks to mere hours. As well, we have seen the quality of near real-time data improve to the point where the distinction starts to blur. We continue to work towards better and faster RFI filtering, adaptive algorithms and improved real-time validation statistics for earlier detection of problems. Can it be possible to produce climate quality data in real-time, and what would the advantages be? We will try to answer these questions…

  12. Analysis and Synthesis of Distributed Real-Time Embedded Systems

    DEFF Research Database (Denmark)

    Pop, Paul; Eles, Petru; Peng, Zebo

    Embedded computer systems are now everywhere: from alarm clocks to PDAs, from mobile phones to cars, almost all the devices we use are controlled by embedded computers. An important class of embedded computer systems is that of hard real-time systems, which have to fulfill strict timing...... in important reductions of design costs. Analysis and Synthesis of Distributed Real-Time Embedded Systems will be of interest to advanced undergraduates, graduate students, researchers and designers involved in the field of embedded systems....

  13. High speed, real-time, camera bandwidth converter

    Science.gov (United States)

    Bower, Dan E; Bloom, David A; Curry, James R

    2014-10-21

    Image data from a CMOS sensor with 10 bit resolution is reformatted in real time to allow the data to stream through communications equipment that is designed to transport data with 8 bit resolution. The incoming image data has 10 bit resolution. The communication equipment can transport image data with 8 bit resolution. Image data with 10 bit resolution is transmitted in real-time, without a frame delay, through the communication equipment by reformatting the image data.

  14. Mechatronic modeling of real-time wheel-rail contact

    CERN Document Server

    Bosso, Nicola; Gugliotta, Antonio; Somà, Aurelio

    2013-01-01

    Real-time simulations of the behaviour of a rail vehicle require realistic solutions of the wheel-rail contact problem which can work in a real-time mode. Examples of such solutions for the online mode have been well known and are implemented within standard and commercial tools for the simulation codes for rail vehicle dynamics. This book is the result of the research activities carried out by the Railway Technology Lab of the Department of Mechanical and Aerospace Engineering at Politecnico di Torino. This book presents work on the project for the development of a real-time wheel-rail contact model and provides the simulation results obtained with dSpace real-time hardware. Besides this, the implementation of the contact model for the development of a real-time model for the complex mechatronic system of a scaled test rig is presented in this book and may be useful for the further validation of the real-time contact model with experiments on a full scale test rig.

  15. Real-time estimation of dynamic functional connectivity networks.

    Science.gov (United States)

    Monti, Ricardo Pio; Lorenz, Romy; Braga, Rodrigo M; Anagnostopoulos, Christoforos; Leech, Robert; Montana, Giovanni

    2017-01-01

    Two novel and exciting avenues of neuroscientific research involve the study of task-driven dynamic reconfigurations of functional connectivity networks and the study of functional connectivity in real-time. While the former is a well-established field within neuroscience and has received considerable attention in recent years, the latter remains in its infancy. To date, the vast majority of real-time fMRI studies have focused on a single brain region at a time. This is due in part to the many challenges faced when estimating dynamic functional connectivity networks in real-time. In this work, we propose a novel methodology with which to accurately track changes in time-varying functional connectivity networks in real-time. The proposed method is shown to perform competitively when compared to state-of-the-art offline algorithms using both synthetic as well as real-time fMRI data. The proposed method is applied to motor task data from the Human Connectome Project as well as to data obtained from a visuospatial attention task. We demonstrate that the algorithm is able to accurately estimate task-related changes in network structure in real-time. Hum Brain Mapp 38:202-220, 2017. © 2016 Wiley Periodicals, Inc.

  16. Real-time feedback from iterative electronic structure calculations.

    Science.gov (United States)

    Vaucher, Alain C; Haag, Moritz P; Reiher, Markus

    2016-04-05

    Real-time feedback from iterative electronic structure calculations requires to mediate between the inherently unpredictable execution times of the iterative algorithm used and the necessity to provide data in fixed and short time intervals for real-time rendering. We introduce the concept of a mediator as a component able to deal with infrequent and unpredictable reference data to generate reliable feedback. In the context of real-time quantum chemistry, the mediator takes the form of a surrogate potential that has the same local shape as the first-principles potential and can be evaluated efficiently to deliver atomic forces as real-time feedback. The surrogate potential is updated continuously by electronic structure calculations and guarantees to provide a reliable response to the operator for any molecular structure. To demonstrate the application of iterative electronic structure methods in real-time reactivity exploration, we implement self-consistent semiempirical methods as the data source and apply the surrogate-potential mediator to deliver reliable real-time feedback.

  17. Real-time Java for on-board systems

    Science.gov (United States)

    Cechticky, V.; Pasetti, A.

    2002-07-01

    The Java language has several attractive features but cannot at present be used in on-board systems primarily because it lacks support for hard real-time operation. This shortcoming is in being addressed: some suppliers are already providing implementations of Java that are RT-compliant; Sun Microsystem has approved a formal specification for a real-time extension of the language; and an independent consortium is working on an alternative specification for real-time Java. It is therefore expected that, within a year or so, standardized commercial implementations of real-time Java will be on the market. Availability of real-time implementations now opens the way to its use on-board. Within this context, this paper has two objectives. Firstly, it discusses the suitability of Java for on-board applications. Secondly, it reports the results of an ESA study to port a software framework for on-board control systems to a commercial real-time version of Java.

  18. Cultivar and Environmental Variation of β-glucan Content in Chinese Barleys

    Institute of Scientific and Technical Information of China (English)

    CHEN Jin-xin; Zhang Guo-ping; QIANG Xiao-lin; WANG Jun-mei; DING Shou-ren

    2002-01-01

    β-glucan is a polysaccharide compound closely related to the quality of barley used as malting,feed and food. Low β-glucan content is expected for brewing and feed barley, while high β-glucan content is desirable for food barley. The β-glucan content of barley genotypes collected from various areas of China as well as from Canada and Australia were assayed. Meanwhile a multi-locations trial was conducted to determineβ-glucan content of 10 barley cultivars in 8 locations for two successive planting years. The results showed that barley genotypes from Tibet and Xinjiang had higher β-glucan content and the genotypes with higher than 8%of β-glucan content were detected in Tibet barleys, being valuable for use in the development of healthy food.Barley cultivars being planted now in winter-sowing areas of China had basically the same β-glucan content as those from Canada and Australia. Barley seeds produced in Hangzhou had lower β-glucan content than seeds from the original areas. There was a highly significant difference in β-glucan content among 10 barleys, 8locations and between years. On an average of two years, Xiumei 3 and Kongpei 1 had the highest and lowestβ-glucan content, respectively, and Taian and Hangzhou produced the highest and lowest β-glucan content barley seeds, respectively. Analysis of AMMI model showed that interaction effect between cultivar and environment was highly significant in both experimental years, and was dependent on cuitivar, suggesting that it is important to plant the suitable cultivars in a particular area in order to obtain barley seeds with reasonableβ-glucan content.

  19. Real Time Fire Reconnaissance Satellite Monitoring System Failure Model

    Science.gov (United States)

    Nino Prieto, Omar Ariosto; Colmenares Guillen, Luis Enrique

    2013-09-01

    In this paper the Real Time Fire Reconnaissance Satellite Monitoring System is presented. This architecture is a legacy of the Detection System for Real-Time Physical Variables which is undergoing a patent process in Mexico. The methodologies for this design are the Structured Analysis for Real Time (SA- RT) [8], and the software is carried out by LACATRE (Langage d'aide à la Conception d'Application multitâche Temps Réel) [9,10] Real Time formal language. The system failures model is analyzed and the proposal is based on the formal language for the design of critical systems and Risk Assessment; AltaRica. This formal architecture uses satellites as input sensors and it was adapted from the original model which is a design pattern for physical variation detection in Real Time. The original design, whose task is to monitor events such as natural disasters and health related applications, or actual sickness monitoring and prevention, as the Real Time Diabetes Monitoring System, among others. Some related work has been presented on the Mexican Space Agency (AEM) Creation and Consultation Forums (2010-2011), and throughout the International Mexican Aerospace Science and Technology Society (SOMECYTA) international congress held in San Luis Potosí, México (2012). This Architecture will allow a Real Time Fire Satellite Monitoring, which will reduce the damage and danger caused by fires which consumes the forests and tropical forests of Mexico. This new proposal, permits having a new system that impacts on disaster prevention, by combining national and international technologies and cooperation for the benefit of humankind.

  20. Geomagnetic Observatory Data for Real-Time Applications

    Science.gov (United States)

    Love, J. J.; Finn, C. A.; Rigler, E. J.; Kelbert, A.; Bedrosian, P.

    2015-12-01

    The global network of magnetic observatories represents a unique collective asset for the scientific community. Historically, magnetic observatories have supported global magnetic-field mapping projects and fundamental research of the Earth's interior and surrounding space environment. More recently, real-time data streams from magnetic observatories have become an important contributor to multi-sensor, operational monitoring of evolving space weather conditions, especially during magnetic storms. In this context, the U.S. Geological Survey (1) provides real-time observatory data to allied space weather monitoring projects, including those of NOAA, the U.S. Air Force, NASA, several international agencies, and private industry, (2) collaborates with Schlumberger to provide real-time geomagnetic data needed for directional drilling for oil and gas in Alaska, (3) develops products for real-time evaluation of hazards for the electric-power grid industry that are associated with the storm-time induction of geoelectric fields in the Earth's conducting lithosphere. In order to implement strategic priorities established by the USGS Natural Hazards Mission Area and the National Science and Technology Council, and with a focus on developing new real-time products, the USGS is (1) leveraging data management protocols already developed by the USGS Earthquake Program, (2) developing algorithms for mapping geomagnetic activity, a collaboration with NASA and NOAA, (3) supporting magnetotelluric surveys and developing Earth conductivity models, a collaboration with Oregon State University and the NSF's EarthScope Program, (4) studying the use of geomagnetic activity maps and Earth conductivity models for real-time estimation of geoelectric fields, (5) initiating geoelectric monitoring at several observatories, (6) validating real-time estimation algorithms against historical geomagnetic and geoelectric data. The success of these long-term projects is subject to funding constraints

  1. Real-Time Tropospheric Delay Estimation using IGS Products

    Science.gov (United States)

    Stürze, Andrea; Liu, Sha; Söhne, Wolfgang

    2014-05-01

    The Federal Agency for Cartography and Geodesy (BKG) routinely provides zenith tropospheric delay (ZTD) parameter for the assimilation in numerical weather models since more than 10 years. Up to now the results flowing into the EUREF Permanent Network (EPN) or E-GVAP (EUMETNET EIG GNSS water vapour programme) analysis are based on batch processing of GPS+GLONASS observations in differential network mode. For the recently started COST Action ES1206 about "Advanced Global Navigation Satellite Systems tropospheric products for monitoring severe weather events and climate" (GNSS4SWEC), however, rapid updates in the analysis of the atmospheric state for nowcasting applications require changing the processing strategy towards real-time. In the RTCM SC104 (Radio Technical Commission for Maritime Services, Special Committee 104) a format combining the advantages of Precise Point Positioning (PPP) and Real-Time Kinematic (RTK) is under development. The so-called State Space Representation approach is defining corrections, which will be transferred in real-time to the user e.g. via NTRIP (Network Transport of RTCM via Internet Protocol). Meanwhile messages for precise orbits, satellite clocks and code biases compatible to the basic PPP mode using IGS products are defined. Consequently, the IGS Real-Time Service (RTS) was launched in 2013 in order to extend the well-known precise orbit and clock products by a real-time component. Further messages e.g. with respect to ionosphere or phase biases are foreseen. Depending on the level of refinement, so different accuracies up to the RTK level shall be reachable. In co-operation of BKG and the Technical University of Darmstadt the real-time software GEMon (GREF EUREF Monitoring) is under development. GEMon is able to process GPS and GLONASS observation and RTS product data streams in PPP mode. Furthermore, several state-of-the-art troposphere models, for example based on numerical weather prediction data, are implemented. Hence, it

  2. Basic technology development of a real-time graphic simulator

    Energy Technology Data Exchange (ETDEWEB)

    Jeon, Jae Wook; Hong, Soon Hyuk; Lee, Kyung Hoon; Lee, Sang-Hyun; Lee, Hee Sub [Sungkyunkwan Univ., Seoul (Korea)

    2001-04-01

    In this research, we developed the basic technology of a Real-time graphic simulator for remote monitoring in hostile environment, the Spent Fuel Disassembling facility. The use of a real-time graphic simulator is very useful for building virtual workcell and simulating the virtual devices to preview the behavior of real devices in order to reduce the error in design step of devices and to evaluate and optimize the work processes. And also, it can be used to provide the visualization of operation information from working devices to ensure the reliable monitoring in the teleoperation system. Thus, the development of basic technology related to these is needed. In this system, because the Spent Fuel Disassembling facility should be managed in remote environment due to its characteristics, the transmission of operational information through the network should be used. And for real-time monitoring, the real-time visualization of operational information from real system should be available. Therefore, we defined the efficient message protocol format for real-time transmission of operational information to visualize these in the 3D graphic simulator. 14 refs., 44 figs., 2 tabs. (Author)

  3. Real-time simulation of thermal shadows with EMIT

    Science.gov (United States)

    Klein, Andreas; Oberhofer, Stefan; Schätz, Peter; Nischwitz, Alfred; Obermeier, Paul

    2016-05-01

    Modern missile systems use infrared imaging for tracking or target detection algorithms. The development and validation processes of these missile systems need high fidelity simulations capable of stimulating the sensors in real-time with infrared image sequences from a synthetic 3D environment. The Extensible Multispectral Image Generation Toolset (EMIT) is a modular software library developed at MBDA Germany for the generation of physics-based infrared images in real-time. EMIT is able to render radiance images in full 32-bit floating point precision using state of the art computer graphics cards and advanced shader programs. An important functionality of an infrared image generation toolset is the simulation of thermal shadows as these may cause matching errors in tracking algorithms. However, for real-time simulations, such as hardware in the loop simulations (HWIL) of infrared seekers, thermal shadows are often neglected or precomputed as they require a thermal balance calculation in four-dimensions (3D geometry in one-dimensional time up to several hours in the past). In this paper we will show the novel real-time thermal simulation of EMIT. Our thermal simulation is capable of simulating thermal effects in real-time environments, such as thermal shadows resulting from the occlusion of direct and indirect irradiance. We conclude our paper with the practical use of EMIT in a missile HWIL simulation.

  4. Spatio-temporal modeling for real-time ozone forecasting.

    Science.gov (United States)

    Paci, Lucia; Gelfand, Alan E; Holland, David M

    2013-05-01

    The accurate assessment of exposure to ambient ozone concentrations is important for informing the public and pollution monitoring agencies about ozone levels that may lead to adverse health effects. High-resolution air quality information can offer significant health benefits by leading to improved environmental decisions. A practical challenge facing the U.S. Environmental Protection Agency (USEPA) is to provide real-time forecasting of current 8-hour average ozone exposure over the entire conterminous United States. Such real-time forecasting is now provided as spatial forecast maps of current 8-hour average ozone defined as the average of the previous four hours, current hour, and predictions for the next three hours. Current 8-hour average patterns are updated hourly throughout the day on the EPA-AIRNow web site. The contribution here is to show how we can substantially improve upon current real-time forecasting systems. To enable such forecasting, we introduce a downscaler fusion model based on first differences of real-time monitoring data and numerical model output. The model has a flexible coefficient structure and uses an efficient computational strategy to fit model parameters. Our hybrid computational strategy blends continuous background updated model fitting with real-time predictions. Model validation analyses show that we are achieving very accurate and precise ozone forecasts.

  5. Lightweight distributed computing for intraoperative real-time image guidance

    Science.gov (United States)

    Suwelack, Stefan; Katic, Darko; Wagner, Simon; Spengler, Patrick; Bodenstedt, Sebastian; Röhl, Sebastian; Dillmann, Rüdiger; Speidel, Stefanie

    2012-02-01

    In order to provide real-time intraoperative guidance, computer assisted surgery (CAS) systems often rely on computationally expensive algorithms. The real-time constraint is especially challenging if several components such as intraoperative image processing, soft tissue registration or context aware visualization are combined in a single system. In this paper, we present a lightweight approach to distribute the workload over several workstations based on the OpenIGTLink protocol. We use XML-based message passing for remote procedure calls and native types for transferring data such as images, meshes or point coordinates. Two different, but typical scenarios are considered in order to evaluate the performance of the new system. First, we analyze a real-time soft tissue registration algorithm based on a finite element (FE) model. Here, we use the proposed approach to distribute the computational workload between a primary workstation that handles sensor data processing and visualization and a dedicated workstation that runs the real-time FE algorithm. We show that the additional overhead that is introduced by the technique is small compared to the total execution time. Furthermore, the approach is used to speed up a context aware augmented reality based navigation system for dental implant surgery. In this scenario, the additional delay for running the computationally expensive reasoning server on a separate workstation is less than a millisecond. The results show that the presented approach is a promising strategy to speed up real-time CAS systems.

  6. Efficient Real-time Video Transmission in Wireless Mesh Network

    Directory of Open Access Journals (Sweden)

    Rajanikanth Aluvalu

    2011-12-01

    Full Text Available We are experiencing rapid improvement in video transmission and picture quality. The technologies are supporting us with real-time video transmissions such as video conferences and webinars. Soon reliable, simple, low cost real-time video will become essential, providing its extensive support to mobiles; PDA’s etc. These enhancements are going to affect the consumer behaviour, business culture. Video transmission includes transmitting packets through Wireless Mesh Network (WMN, which turned out to be a challenging scenario for real-time video services. Due to the existing nature of wireless networks include the presence of unpredictable delays and high packet error rates due to the error-prone nature of the wireless links. In this paper, we will propose a model which provides high capacity data transfer with reduced delay. We have simulated and achieved results with higher through put.

  7. Real time capable infrared thermography for ASDEX Upgrade

    Energy Technology Data Exchange (ETDEWEB)

    Sieglin, B., E-mail: Bernhard.Sieglin@ipp.mpg.de; Faitsch, M.; Herrmann, A.; Brucker, B.; Eich, T.; Kammerloher, L.; Martinov, S. [Max-Planck Institute for Plasma Physics, Boltzmannstr. 2, D-85748 Garching (Germany)

    2015-11-15

    Infrared (IR) thermography is widely used in fusion research to study power exhaust and incident heat load onto the plasma facing components. Due to the short pulse duration of today’s fusion experiments, IR systems have mostly been designed for off-line data analysis. For future long pulse devices (e.g., Wendelstein 7-X, ITER), a real time evaluation of the target temperature and heat flux is mandatory. This paper shows the development of a real time capable IR system for ASDEX Upgrade. A compact IR camera has been designed incorporating the necessary magnetic and electric shielding for the detector, cooler assembly. The camera communication is based on the Camera Link industry standard. The data acquisition hardware is based on National Instruments hardware, consisting of a PXIe chassis inside and a fibre optical connected industry computer outside the torus hall. Image processing and data evaluation are performed using real time LabVIEW.

  8. Real-time structured light intraoral 3D measurement pipeline

    Science.gov (United States)

    Gheorghe, Radu; Tchouprakov, Andrei; Sokolov, Roman

    2013-02-01

    Computer aided design and manufacturing (CAD/CAM) is increasingly becoming a standard feature and service provided to patients in dentist offices and denture manufacturing laboratories. Although the quality of the tools and data has slowly improved in the last years, due to various surface measurement challenges, practical, accurate, invivo, real-time 3D high quality data acquisition and processing still needs improving. Advances in GPU computational power have allowed for achieving near real-time 3D intraoral in-vivo scanning of patient's teeth. We explore in this paper, from a real-time perspective, a hardware-software-GPU solution that addresses all the requirements mentioned before. Moreover we exemplify and quantify the hard and soft deadlines required by such a system and illustrate how they are supported in our implementation.

  9. A real-time VLC to UART protocol conversion system

    Science.gov (United States)

    Deng, Jian-zhi; Yao, Meng; Cheng, Xiao-hui; Deng, Zhuo-hong

    2016-07-01

    A real-time visible light communication (VLC) to universal asynchronous receiver/transmitter (UART) conversion system is made up of a transmitter with a light emitting diode (LED) and a receiver with a photodiode (PD), by which a VLC system is connected to traditional communication modes, and the data are transferred by wireless visible light. UART packets are converted to light packets by the modulation of a 10 kHz on-off-keying (OOK) light signal, and the data losses in the transportation are avoided by the protection of a data buffer mechanism. The experimental results reveal that the real-time VLC to UART conversion system can provide a real-time VLC transmission way for two UART devices in not less than 10 m at a baud rate not less than 19 200 Bd with stable ambient lighting at the same time.

  10. Reduction Methods for Real-time Simulations in Hybrid Testing

    DEFF Research Database (Denmark)

    Andersen, Sebastian

    2016-01-01

    Hybrid testing constitutes a cost-effective experimental full scale testing method. The method was introduced in the 1960's by Japanese researchers, as an alternative to conventional full scale testing and small scale material testing, such as shake table tests. The principle of the method...... is to divide a structure into a physical substructure and a numerical substructure, and couple these in a test. If the test is conducted in real-time it is referred to as real time hybrid testing. The hybrid testing concept has developed significantly since its introduction in the 1960', both with respect...... without introducing further unknowns into the system. The basis formulation is shown to exhibit high precision and to reduce the computational cost significantly. Furthermore, the basis formulation exhibits a significant higher stability, than standard nonlinear algorithms. A real-time hybrid test...

  11. Compiling graphical real-time specifications into silicon

    DEFF Research Database (Denmark)

    Fränzle, Martin; Lüth, Karsten

    1998-01-01

    The basic algorithms underlying an automatic hardware synthesis environment using fully formal graphical requirements specifications as source language are outlined. The source language is real-time symbolic timing diagrams [FeyerabendJosko97], which are a metric-time temporal logic such that hard...... real-time constraints have to be dealt with. While automata-theoretic methods based on translating the specification to a finite automaton and constructing a winning strategy in the resulting omega-regular game could in principle be used, and do indeed provide the core algorithm, complexity withstands...... practical application of these methods. Therefore, a compositional extension is explored, which yields modular synthesis of multi-component controllers. Based on this, a second extension is proposed for efficiently dealing with hard real-time constraints....

  12. Real-time Flame Rendering with GPU and CUDA

    Directory of Open Access Journals (Sweden)

    Wei Wei

    2011-02-01

    Full Text Available This paper proposes a method of flame simulation based on Lagrange process and chemical composition, which was non-grid and the problems associated with there grids were overcome. The turbulence movement of flame was described by Lagrange process and chemical composition was added into flame simulation which increased the authenticity of flame. For real-time applications, this paper simplified the EMST model. GPU-based particle system combined with OpenGL VBO and PBO unique technology was used to accelerate finally, the speed of vertex and pixel data interaction between CPU and GPU increased two orders of magnitude, frame rate of rendering increased by 30%, which achieved fast dynamic flame real-time simulation. For further real-time applications, this paper presented a strategy to implement flame simulation with CUDA on GPU, which achieved a speed up to 2.5 times the previous implementation.

  13. REAL TIME FACE RECOGNITION USING ADABOOST IMPROVED FAST PCA ALGORITHM

    Directory of Open Access Journals (Sweden)

    K. Susheel Kumar

    2011-08-01

    Full Text Available This paper presents an automated system for human face recognition in a real time background world fora large homemade dataset of persons face. The task is very difficult as the real time backgroundsubtraction in an image is still a challenge. Addition to this there is a huge variation in human face imagein terms of size, pose and expression. The system proposed collapses most of this variance. To detect realtime human face AdaBoost with Haar cascade is used and a simple fast PCA and LDA is used torecognize the faces detected. The matched face is then used to mark attendance in the laboratory, in ourcase. This biometric system is a real time attendance system based on the human face recognition with asimple and fast algorithms and gaining a high accuracy rate..

  14. Analysis of hybrid viscous damper by real time hybrid simulations

    DEFF Research Database (Denmark)

    Brodersen, Mark Laier; Ou, Ge; Høgsberg, Jan Becker

    2016-01-01

    Results from real time hybrid simulations are compared to full numerical simulations for a hybrid viscous damper, composed of a viscous dashpot in series with an active actuator and a load cell. By controlling the actuator displacement via filtered integral force feedback the damping performance...... of the hybrid viscous damper is improved, while for pure integral force feedback the damper stroke is instead increased. In the real time hybrid simulations viscous damping is emulated by a bang-bang controlled Magneto-Rheological (MR) damper. The controller activates high-frequency modes and generates drift...... in the actuator displacement, and only a fraction of the measured damper force can therefore be used as input to the investigated integral force feedback in the real time hybrid simulations....

  15. Analysis and Optimization of Distributed Real-Time Embedded Systems

    DEFF Research Database (Denmark)

    Pop, Paul; Eles, Petru; Peng, Zebo

    2006-01-01

    for such heterogeneous distributed real-time embedded systems. More specifically, we discuss the schedulability analysis of hard real-time systems, highlighting particular aspects related to the heterogeneous and distributed nature of the applications. We also introduce several design optimization problems......An increasing number of real-time applications are today implemented using distributed heterogeneous architectures composed of interconnected networks of processors. The systems are heterogeneous not only in terms of hardware and software components, but also in terms of communication protocols...... and scheduling policies. In this context, the task of designing such systems is becoming increasingly difficult. The success of new adequate design methods depends on the availability of efficient analysis as well as optimization techniques. In this paper, we present both analysis and optimization approaches...

  16. An optimal real-time controller for vertical plasma stabilization

    CERN Document Server

    Cruz, N; Coda, S; Duval, B P; Le, H B; Rodrigues, A P; Varandas, C A F; Correia, C M B A; Goncalves, B S

    2014-01-01

    Modern Tokamaks have evolved from the initial axisymmetric circular plasma shape to an elongated axisymmetric plasma shape that improves the energy confinement time and the triple product, which is a generally used figure of merit for the conditions needed for fusion reactor performance. However, the elongated plasma cross section introduces a vertical instability that demands a real-time feedback control loop to stabilize the plasma vertical position and velocity. At the Tokamak \\`a Configuration Variable (TCV) in-vessel poloidal field coils driven by fast switching power supplies are used to stabilize highly elongated plasmas. TCV plasma experiments have used a PID algorithm based controller to correct the plasma vertical position. In late 2013 experiments a new optimal real-time controller was tested improving the stability of the plasma. This contribution describes the new optimal real-time controller developed. The choice of the model that describes the plasma response to the actuators is discussed. The ...

  17. FPGA Implementation of Real-Time Ethernet for Motion Control

    Directory of Open Access Journals (Sweden)

    Chen Youdong

    2013-01-01

    Full Text Available This paper provides an applicable implementation of real-time Ethernet named CASNET, which modifies the Ethernet medium access control (MAC to achieve the real-time requirement for motion control. CASNET is the communication protocol used for motion control system. Verilog hardware description language (VHDL has been used in the MAC logic design. The designed MAC serves as one of the intellectual properties (IPs and is applicable to various industrial controllers. The interface of the physical layer is RJ45. The other layers have been implemented by using C programs. The real-time Ethernet has been implemented by using field programmable gate array (FPGA technology and the proposed solution has been tested through the cycle time, synchronization accuracy, and Wireshark testing.

  18. Real time lobster posture estimation for behavior research

    Science.gov (United States)

    Yan, Sheng; Alfredsen, Jo Arve

    2017-02-01

    In animal behavior research, the main task of observing the behavior of an animal is usually done manually. The measurement of the trajectory of an animal and its real-time posture description is often omitted due to the lack of automatic computer vision tools. Even though there are many publications for pose estimation, few are efficient enough to apply in real-time or can be used without the machine learning algorithm to train a classifier from mass samples. In this paper, we propose a novel strategy for the real-time lobster posture estimation to overcome those difficulties. In our proposed algorithm, we use the Gaussian mixture model (GMM) for lobster segmentation. Then the posture estimation is based on the distance transform and skeleton calculated from the segmentation. We tested the algorithm on a serials lobster videos in different size and lighting conditions. The results show that our proposed algorithm is efficient and robust under various conditions.

  19. The VLBA Correlator---Real-Time in the Distributed ERA

    Science.gov (United States)

    Wells, Donald C.

    1993-01-01

    The Correlator is the signal processing engine of the Very Long Baseline Array [VLBA]. Radio signals are recorded on special wideband digital recorders at the 10 VLBA antennas and are shipped to the Array Operations Center in Socorro, New Mexico, where they are played back simultaneously into the Correlator. Real-time software and firmware controls the playback drives to achieve synchronization, compute models of the wavefront delay, control the numerous modules of the Correlator, and record FITS files of the fringe visibilities at the back-end of the Correlator. The Correlator system contains a total of more than 100 programmable computers, which communicate by means of various protocols. The VLBA Correlator's dependence on network protocols is an example of the radical transformation of the real-time world over the past five years: real-time is becoming more like conventional computing.

  20. Real-time simulation of large-scale floods

    Science.gov (United States)

    Liu, Q.; Qin, Y.; Li, G. D.; Liu, Z.; Cheng, D. J.; Zhao, Y. H.

    2016-08-01

    According to the complex real-time water situation, the real-time simulation of large-scale floods is very important for flood prevention practice. Model robustness and running efficiency are two critical factors in successful real-time flood simulation. This paper proposed a robust, two-dimensional, shallow water model based on the unstructured Godunov- type finite volume method. A robust wet/dry front method is used to enhance the numerical stability. An adaptive method is proposed to improve the running efficiency. The proposed model is used for large-scale flood simulation on real topography. Results compared to those of MIKE21 show the strong performance of the proposed model.

  1. Games and Scenarios for Real-Time System Validation

    DEFF Research Database (Denmark)

    Li, Shuhao

    communicating real-time systems can be modeled and specified with LSC. By translating LSC to timed automata (TAs), we reduce scenario-based model consistency checking and property verification to CTL real-time model checking problems, and reduce scenario-based synthesis to a timed game solving problem...... game between the tester and the system under test (SUT). We propose methods of using winning strategies as test cases for black-box conformance testing. The methods are generalized to problems where only possibly winning game strategies can be obtained. In this case continued testing requires some......This thesis presents research on the validation of real-time embedded software systems in the context of model-based development. The thesis proposes scenario-based and game-theoretic approaches to system analysis, verification, synthesis and testing to address the challenges that arise from...

  2. Real-Time Parameter Estimation Using Output Error

    Science.gov (United States)

    Grauer, Jared A.

    2014-01-01

    Output-error parameter estimation, normally a post- ight batch technique, was applied to real-time dynamic modeling problems. Variations on the traditional algorithm were investigated with the goal of making the method suitable for operation in real time. Im- plementation recommendations are given that are dependent on the modeling problem of interest. Application to ight test data showed that accurate parameter estimates and un- certainties for the short-period dynamics model were available every 2 s using time domain data, or every 3 s using frequency domain data. The data compatibility problem was also solved in real time, providing corrected sensor measurements every 4 s. If uncertainty corrections for colored residuals are omitted, this rate can be increased to every 0.5 s.

  3. Congestion Management Strategies of Real-Time Market

    DEFF Research Database (Denmark)

    Wang, Qi; Zhang, Chunyu; Ding, Yi

    2014-01-01

    The high penetration of distributed energy resources (DERs) will significantly challenge the power system operation and control due to their stochastic, intermittent, and fluctuation characteristics. This enhances the difficulty of congestion management of power systems in cross-border electricity...... market among different regions. For handling this, the Real-Time Market is proposed for balancing capacity trading against congestions. Several strategies for Real-Time Market dealing with congestions are proposed. The strategy of two-stage crossborder markets in Day-ahead, Intra-day and Real Time Market...... are introduced with the congestion constraints complied. Pre-Contingency strategy is proposed as the advance preparation for the future congestion, and In-Day re-dispatch is used for regulation. Accordingly, the requirements on facilities considering telemetry and remote control in a fast manner are discussed...

  4. Real-Time Statistical Modeling of Blood Sugar.

    Science.gov (United States)

    Otoom, Mwaffaq; Alshraideh, Hussam; Almasaeid, Hisham M; López-de-Ipiña, Diego; Bravo, José

    2015-10-01

    Diabetes is considered a chronic disease that incurs various types of cost to the world. One major challenge in the control of Diabetes is the real time determination of the proper insulin dose. In this paper, we develop a prototype for real time blood sugar control, integrated with the cloud. Our system controls blood sugar by observing the blood sugar level and accordingly determining the appropriate insulin dose based on patient's historical data, all in real time and automatically. To determine the appropriate insulin dose, we propose two statistical models for modeling blood sugar profiles, namely ARIMA and Markov-based model. Our experiment used to evaluate the performance of the two models shows that the ARIMA model outperforms the Markov-based model in terms of prediction accuracy.

  5. Intelligent data management for real-time spacecraft monitoring

    Science.gov (United States)

    Schwuttke, Ursula M.; Gasser, Les; Abramson, Bruce

    1992-01-01

    Real-time AI systems have begun to address the challenge of restructuring problem solving to meet real-time constraints by making key trade-offs that pursue less than optimal strategies with minimal impact on system goals. Several approaches for adapting to dynamic changes in system operating conditions are known. However, simultaneously adapting system decision criteria in a principled way has been difficult. Towards this end, a general technique for dynamically making such trade-offs using a combination of decision theory and domain knowledge has been developed. Multi-attribute utility theory (MAUT), a decision theoretic approach for making one-time decisions is discussed and dynamic trade-off evaluation is described as a knowledge-based extension of MAUT that is suitable for highly dynamic real-time environments, and provides an example of dynamic trade-off evaluation applied to a specific data management trade-off in a real-world spacecraft monitoring application.

  6. Explaining How to Play Real-Time Strategy Games

    Science.gov (United States)

    Metoyer, Ronald; Stumpf, Simone; Neumann, Christoph; Dodge, Jonathan; Cao, Jill; Schnabel, Aaron

    Real-time strategy games share many aspects with real situations in domains such as battle planning, air traffic control, and emergency response team management which makes them appealing test-beds for Artificial Intelligence (AI) and machine learning. End user annotations could help to provide supplemental information for learning algorithms, especially when training data is sparse. This paper presents a formative study to uncover how experienced users explain game play in real-time strategy games. We report the results of our analysis of explanations and discuss their characteristics that could support the design of systems for use by experienced real-time strategy game users in specifying or annotating strategy-oriented behavior.

  7. Specifying and verifying requirements of real-time systems

    DEFF Research Database (Denmark)

    Ravn, Anders P.; Rischel, Hans; Hansen, Kirsten Mark

    1993-01-01

    An approach to specification of requirements and verification of design for real-time systems is presented. A system is defined by a conventional mathematical model for a dynamic system where application specific states denote functions of real time. Specifications are formulas in duration calculus......, a real-time interval logic, where predicates define durations of states. Requirements define safety and functionality constraints on the system or a component. A top-level design is given by a control law: a predicate that defines an automation controlling the transition between phases of operation. Each...... control the distributed computation through synchronous events. Sensors and actuators relate events with system states. Verification is a deduction showing that a design implies requirements...

  8. A Simplification of a Real-Time Verification Problem

    CERN Document Server

    Saha, Indranil; Roy, Suman; 10.1007/978-3-540-75596-8_21

    2010-01-01

    We revisit the problem of real-time verification with dense dynamics using timeout and calendar based models and simplify this to a finite state verification problem. To overcome the complexity of verification of real-time systems with dense dynamics, Dutertre and Sorea, proposed timeout and calender based transition systems to model the behavior of real-time systems and verified safety properties using k-induction in association with bounded model checking. In this work, we introduce a specification formalism for these models in terms of Timeout Transition Diagrams and capture their behavior in terms of semantics of Timed Transition Systems. Further, we discuss a technique, which reduces the problem of verification of qualitative temporal properties on infinite state space of (a large fragment of) these timeout and calender based transition systems into that on clockless finite state models through a two-step process comprising of digitization and canonical finitary reduction. This technique enables us to ve...

  9. Real time capable infrared thermography for ASDEX Upgrade

    Science.gov (United States)

    Sieglin, B.; Faitsch, M.; Herrmann, A.; Brucker, B.; Eich, T.; Kammerloher, L.; Martinov, S.

    2015-11-01

    Infrared (IR) thermography is widely used in fusion research to study power exhaust and incident heat load onto the plasma facing components. Due to the short pulse duration of today's fusion experiments, IR systems have mostly been designed for off-line data analysis. For future long pulse devices (e.g., Wendelstein 7-X, ITER), a real time evaluation of the target temperature and heat flux is mandatory. This paper shows the development of a real time capable IR system for ASDEX Upgrade. A compact IR camera has been designed incorporating the necessary magnetic and electric shielding for the detector, cooler assembly. The camera communication is based on the Camera Link industry standard. The data acquisition hardware is based on National Instruments hardware, consisting of a PXIe chassis inside and a fibre optical connected industry computer outside the torus hall. Image processing and data evaluation are performed using real time LabVIEW.

  10. High performance real-time flight simulation at NASA Langley

    Science.gov (United States)

    Cleveland, Jeff I., II

    1994-01-01

    In order to meet the stringent time-critical requirements for real-time man-in-the-loop flight simulation, computer processing operations must be deterministic and be completed in as short a time as possible. This includes simulation mathematical model computational and data input/output to the simulators. In 1986, in response to increased demands for flight simulation performance, personnel at NASA's Langley Research Center (LaRC), working with the contractor, developed extensions to a standard input/output system to provide for high bandwidth, low latency data acquisition and distribution. The Computer Automated Measurement and Control technology (IEEE standard 595) was extended to meet the performance requirements for real-time simulation. This technology extension increased the effective bandwidth by a factor of ten and increased the performance of modules necessary for simulator communications. This technology is being used by more than 80 leading technological developers in the United States, Canada, and Europe. Included among the commercial applications of this technology are nuclear process control, power grid analysis, process monitoring, real-time simulation, and radar data acquisition. Personnel at LaRC have completed the development of the use of supercomputers for simulation mathematical model computational to support real-time flight simulation. This includes the development of a real-time operating system and the development of specialized software and hardware for the CAMAC simulator network. This work, coupled with the use of an open systems software architecture, has advanced the state of the art in real time flight simulation. The data acquisition technology innovation and experience with recent developments in this technology are described.

  11. New real time needle segmentation technique using grayscale Hough transformation

    Science.gov (United States)

    Qiu, Wu; Zhou, Hua; Ding, Mingyue; Zhang, Songgeng

    2007-12-01

    Real-time needle segmentation and tracking is very important in image-guided surgery, biopsy, and therapy. In this paper, we described an automated technique to provide real-time needle segmentation from a sequence of 2-D ultrasound images for the use of guidance of a needle to the target in soft tissues. The Hough transform is used to find straight lines or analytic curves in binary image. Hough transform is applied usually to binary images. Hence one needs to convert, initially, the gray level image to a binary one (through thresholding, edge detection, or thinning) in order to apply the HT. While in the process of binarization, some information about line segments in the image may be lost when an inappropriate threshold is used. Gray-Scale Hough Transform can detect the line without binarization. Unfortunately, its high computational cost often prevents it from being applied in real-time applications without the help of specially designed hardware. In this paper, we proposed a needle segmentation technique based on a real-time gray-scale Hough transform. It is composed of an improved Gray Hough Transformation and a coarse-fine search strategy. Furthermore, the RTGHT (Real-Time Gray-Scale Hough Transform) technique is evaluated by patient breast biopsy images. Experiments with patient breast biopsy ultrasound (US) image sequences showed that our approach can segment the biopsy needle in real time (i.e., less than 60 ms) with the angular rms error of about 1° and the position rms error of about 0.5 mm an affordable PC computer without the help of specially designed hardware.

  12. Simultaneous real-time monitoring of multiple cortical systems

    Science.gov (United States)

    Gupta, Disha; Hill, N. Jeremy; Brunner, Peter; Gunduz, Aysegul; Ritaccio, Anthony L.; Schalk, Gerwin

    2014-10-01

    Objective. Real-time monitoring of the brain is potentially valuable for performance monitoring, communication, training or rehabilitation. In natural situations, the brain performs a complex mix of various sensory, motor or cognitive functions. Thus, real-time brain monitoring would be most valuable if (a) it could decode information from multiple brain systems simultaneously, and (b) this decoding of each brain system were robust to variations in the activity of other (unrelated) brain systems. Previous studies showed that it is possible to decode some information from different brain systems in retrospect and/or in isolation. In our study, we set out to determine whether it is possible to simultaneously decode important information about a user from different brain systems in real time, and to evaluate the impact of concurrent activity in different brain systems on decoding performance. Approach. We study these questions using electrocorticographic signals recorded in humans. We first document procedures for generating stable decoding models given little training data, and then report their use for offline and for real-time decoding from 12 subjects (six for offline parameter optimization, six for online experimentation). The subjects engage in tasks that involve movement intention, movement execution and auditory functions, separately, and then simultaneously. Main results. Our real-time results demonstrate that our system can identify intention and movement periods in single trials with an accuracy of 80.4% and 86.8%, respectively (where 50% would be expected by chance). Simultaneously, the decoding of the power envelope of an auditory stimulus resulted in an average correlation coefficient of 0.37 between the actual and decoded power envelopes. These decoders were trained separately and executed simultaneously in real time. Significance. This study yielded the first demonstration that it is possible to decode simultaneously the functional activity of multiple

  13. Real time microcontroller implementation of an adaptive myoelectric filter.

    Science.gov (United States)

    Bagwell, P J; Chappell, P H

    1995-03-01

    This paper describes a real time digital adaptive filter for processing myoelectric signals. The filter time constant is automatically selected by the adaptation algorithm, giving a significant improvement over linear filters for estimating the muscle force and controlling a prosthetic device. Interference from mains sources often produces problems for myoelectric processing, and so 50 Hz and all harmonic frequencies are reduced by an averaging filter and differential process. This makes practical electrode placement and contact less critical and time consuming. An economic real time implementation is essential for a prosthetic controller, and this is achieved using an Intel 80C196KC microcontroller.

  14. Magneto-optical system for high speed real time imaging

    Science.gov (United States)

    Baziljevich, M.; Barness, D.; Sinvani, M.; Perel, E.; Shaulov, A.; Yeshurun, Y.

    2012-08-01

    A new magneto-optical system has been developed to expand the range of high speed real time magneto-optical imaging. A special source for the external magnetic field has also been designed, using a pump solenoid to rapidly excite the field coil. Together with careful modifications of the cryostat, to reduce eddy currents, ramping rates reaching 3000 T/s have been achieved. Using a powerful laser as the light source, a custom designed optical assembly, and a high speed digital camera, real time imaging rates up to 30 000 frames per seconds have been demonstrated.

  15. Integration of Real-Time Data Into Building Automation Systems

    Energy Technology Data Exchange (ETDEWEB)

    Mark J. Stunder; Perry Sebastian; Brenda A. Chube; Michael D. Koontz

    2003-04-16

    The project goal was to investigate the possibility of using predictive real-time information from the Internet as an input to building management system algorithms. The objectives were to identify the types of information most valuable to commercial and residential building owners, managers, and system designers. To comprehensively investigate and document currently available electronic real-time information suitable for use in building management systems. Verify the reliability of the information and recommend accreditation methods for data and providers. Assess methodologies to automatically retrieve and utilize the information. Characterize equipment required to implement automated integration. Demonstrate the feasibility and benefits of using the information in building management systems. Identify evolutionary control strategies.

  16. Handheld portable real-time tracking and communications device

    Energy Technology Data Exchange (ETDEWEB)

    Wiseman, James M [Albuquerque, NM; Riblett, Jr., Loren E. (Edgewood, NM); Green, Karl L [Albuquerque, NM; Hunter, John A [Albuquerque, NM; Cook, III, Robert N. (Rio Rancho, NM); Stevens, James R [Arlington, VA

    2012-05-22

    Portable handheld real-time tracking and communications devices include; a controller module, communications module including global positioning and mesh network radio module, data transfer and storage module, and a user interface module enclosed in a water-resistant enclosure. Real-time tracking and communications devices can be used by protective force, security and first responder personnel to provide situational awareness allowing for enhance coordination and effectiveness in rapid response situations. Such devices communicate to other authorized devices via mobile ad-hoc wireless networks, and do not require fixed infrastructure for their operation.

  17. Real-time alerts and reminders using information systems.

    Science.gov (United States)

    Wanderer, Jonathan P; Sandberg, Warren S; Ehrenfeld, Jesse M

    2011-09-01

    Adoption of information systems throughout the hospital environment has enabled the development of real-time physiologic alerts and clinician reminder systems. These clinical tools can be made available through the deployment of anesthesia information management systems (AIMS). Creating usable alert systems requires understanding of technical considerations. Various successful implementations are reviewed, encompassing cost reduction, improved revenue capture, timely antibiotic administration, and postoperative nausea and vomiting prophylaxis. Challenges to the widespread use of real-time alerts and reminders include AIMS adoption rates and the difficulty in choosing appropriate areas and approaches for information systems support.

  18. The Challenges Of Using Multicomputing In Real Time Visualapplications

    Directory of Open Access Journals (Sweden)

    Ajay Anant Joshi

    2011-12-01

    Full Text Available Displaying visual data in a graphical distributed application is one of the main challenges and constraintsthat should be studied in real time applications. Many problems could be reported in using multicomputingfor real time visual applications. These problems are identified throughout this survey, and areclassified into two main categories. The first is computation and transfer time while the second is displayspeed. The real bottleneck in using grid or cloud computing in such applications is whether thecommunication time between nodes (inside the cloud increases to the point of degrading overallapplication performance. This survey paper describes the impact of time and communication speed onapplying visual systems on a grid or in cloud computing.

  19. Towards real time diagnostics of Hybrid Welding Laser/GMAW

    Energy Technology Data Exchange (ETDEWEB)

    McJunkin, T. R.; Kunerth, D. C.; Nichol, C. I. [Idaho National Laboratory, Idaho Falls, ID 83415-3570 (United States); Todorov, E.; Levesque, S. [Edison Welding Institute, Columbus, OH (United States)

    2014-02-18

    Methods are currently being developed towards a more robust system real time feedback in the high throughput process combining laser welding with gas metal arc welding. A combination of ultrasonic, eddy current, electronic monitoring, and visual techniques are being applied to the welding process. Initial simulation and bench top evaluation of proposed real time techniques on weld samples are presented along with the concepts to apply the techniques concurrently to the weld process. Consideration for the eventual code acceptance of the methods and system are also being researched as a component of this project. The goal is to detect defects or precursors to defects and correct when possible during the weld process.

  20. Organic semiconductors as real-time radiation detectors

    Energy Technology Data Exchange (ETDEWEB)

    Suzuki, T., E-mail: tsuzuki@hep.sc.niigata-u.ac.jp [Graduate School of Science and Technology, Niigata University, Niigata 950-2181 (Japan); Miyata, H., E-mail: miyata@hep.sc.niigata-u.ac.jp [Department of Physics, Niigata University, Niigata 950-2181 (Japan); Katsumata, M. [Department of Physics, Niigata University, Niigata 950-2181 (Japan); Nakano, S.; Matsuda, K. [Graduate School of Science and Technology, Niigata University, Niigata 950-2181 (Japan); Tamura, M. [Carlit Holdings Co., Ltd., Tokyo 104-0031 (Japan)

    2014-11-01

    In this study, the possibility of using π-conjugated organic semiconducting polymers as real-time radiation detectors was explored. Polyaniline (PAni) was used to fabricate radiation sensors because of its relative long-term stability in air. Each fabricated sensor was then subjected to irradiation by α- and β-particles, and the real-time response was measured. The multichannel analyzer (MCA) data of the response signal for each irradiation was acquired and the detection efficiency, relative to the electrode bias voltage of the detector, was extracted.

  1. Atomic Stretch: Optimally bounded real-time stretching and beyond

    DEFF Research Database (Denmark)

    Jensen, Rasmus Ramsbøl; Nielsen, Jannik Boll

    2016-01-01

    Atomic Stretch is a plugin for your preferred Adobe video editing tool, allowing real-time smooth and optimally bounded retarget-ting from and to any aspect ratio. The plugin allows preserving of high interest pixels through a protected region, attention redirection through color-modification, co......Atomic Stretch is a plugin for your preferred Adobe video editing tool, allowing real-time smooth and optimally bounded retarget-ting from and to any aspect ratio. The plugin allows preserving of high interest pixels through a protected region, attention redirection through color......-modification, countering barrelling effects through vertical stretching, and tracking of targets of interest....

  2. An Intelligent Real-Time System Architecture Implemented in ADA

    Science.gov (United States)

    1992-12-01

    performed by the system is procedural in nature [ Wilber , 1989:75]. For example, if one was to create a fully autonomous system to pilot a modem fighter...Tindell, Ken , Bums, Alan, and Wellings, Andy, Allocating Hard Real Time Tasks (An NP-Hard Problem Made Easy), e-mail via ftp, 1992, Real Time Systems...Technology/ENS Wright-Patterson AFB, Ohio 45433-6583, Distribution Limited to DoD and DoD contractors only, April 1990. [ Wilber , 1989]. Wilber , George

  3. Real Time Engineering Analysis Based on a Generative Component Implementation

    DEFF Research Database (Denmark)

    Kirkegaard, Poul Henning; Klitgaard, Jens

    2007-01-01

    The present paper outlines the idea of a conceptual design tool with real time engineering analysis which can be used in the early conceptual design phase. The tool is based on a parametric approach using Generative Components with embedded structural analysis. Each of these components uses...... without jumping from aesthetics to structural digital design tools and back, but to work with both simultaneously and real time. The engineering level of knowledge is incorporated at a conceptual thinking level, i.e. qualitative information is used in stead of using quantitative information. An example...

  4. High dynamic range real-time 3D shape measurement.

    Science.gov (United States)

    Jiang, Chufan; Bell, Tyler; Zhang, Song

    2016-04-04

    This paper proposes a method that can measure high-contrast surfaces in real-time without changing camera exposures. We propose to use 180-degree phase-shifted (or inverted) fringe patterns to complement regular fringe patterns. If not all of the regular patterns are saturated, inverted fringe patterns are used in lieu of original saturated patterns for phase retrieval, and if all of the regular fringe patterns are saturated, both the original and inverted fringe patterns are all used for phase computation to reduce phase error. Experimental results demonstrate that three-dimensional (3D) shape measurement can be achieved in real time by adopting the proposed high dynamic range method.

  5. A heterogeneous hierarchical architecture for real-time computing

    Energy Technology Data Exchange (ETDEWEB)

    Skroch, D.A.; Fornaro, R.J.

    1988-12-01

    The need for high-speed data acquisition and control algorithms has prompted continued research in the area of multiprocessor systems and related programming techniques. The result presented here is a unique hardware and software architecture for high-speed real-time computer systems. The implementation of a prototype of this architecture has required the integration of architecture, operating systems and programming languages into a cohesive unit. This report describes a Heterogeneous Hierarchial Architecture for Real-Time (H{sup 2} ART) and system software for program loading and interprocessor communication.

  6. Real-time PCR in Food Science: Introduction.

    Science.gov (United States)

    Rodriguez-Lazaro, David; Hernandez, Marta

    2013-01-01

    Food safety and quality control programmes are increasingly applied throughout the production food chain in order to guarantee added value products as well as to minimize the risk of infection for the consumer. The development of real-time PCR has represented one of the most significant advances in food diagnostics as it provides rapid, reliable and quantitative results. These aspects become increasingly important for the agricultural and food industry. Different strategies for real-time PCR diagnostics have been developed including unspecific detection independent of the target sequence using fluorescent dyes such as SYBR Green, or by sequence-specific fluorescent oligonucleotide probes such as TaqMan probes or molecular beacons.

  7. Dynamic real-time hierarchical heuristic search for pathfinding.

    OpenAIRE

    Naveed, Munir; Kitchin, Diane E.; Crampton, Andrew

    2009-01-01

    Movement of Units in Real-Time Strategy (RTS) Games is a non-trivial and challenging task mainly due to three factors which are constraints on CPU and memory usage, dynamicity of the game world, and concurrency. In this paper, we are focusing on finding a novel solution for solving the pathfinding problem in RTS Games for the units which are controlled by the computer. The novel solution combines two AI Planning approaches: Hierarchical Task Network (HTN) and Real-Time Heuristic Search (RHS)....

  8. A real time method for surface cleanliness measurement

    OpenAIRE

    Bilmes, Gabriel Mario; Orzi, Daniel Jesús Omar; Martínez , Oscar E.; Lencina, Alberto

    2006-01-01

    The measurement of surface cleanliness is a signifi cant problem in many industrial and technological processes. Existing methods are based on laboratory procedures, that are not performed in real time, can not be automated, and usually are restricted to a small portion of the sample. In this study we describe a new method for real time measurement of the amount of surface dirt or contamination deposited on a surface. It relies on the ablation of the surface dirt film by means of a short l...

  9. Steerable real-time sonographically guided needle biopsy.

    Science.gov (United States)

    Buonocore, E; Skipper, G J

    1981-02-01

    A method for dynamic real-time ultrasonic guidance for percutaneous needle biopsy has been successful in obtaining cytologic and histologic specimens from abdominal masses. The system depends on a real-time ultrasonic transducer that has been rigidly attached to a laterally placed steerable needle holder. Using simple trigonometric functions, a chart has been derived that gives the exact angulation and needle length to produce quick, reliable, guided needle placements. Examples of successful renal, hepatobiliary, and retroperitoneal biopsies are presented. Advantages of this technique include speed, accuracy, low cost, three-dimensional format, and the omission of contrast media and radiation.

  10. System security assessment in real-time using synchrophasor measurements

    DEFF Research Database (Denmark)

    Jóhannsson, Hjörtur; Wache, Markus

    2013-01-01

    assessment and sheds light on ongoing research activities that focus on exploiting wide-area synchrophasor measurements for real-time security assessment of sustainable power systems. At last, an mathematical mapping enabling informative visualization of the system state in respect to aperiodic rotor angle...... measures to ensure stable and secure operation of the system are necessary. Time stamped synchrophasor measurements lay the foundation for development of new real-time applications for security and stability assessment. The paper provides overview of existing solutions for synchrophasor based security...

  11. Real time visual tracking of targets in six dimensions

    Science.gov (United States)

    Walsh, Peter M.; Shawaga, Larry

    1990-01-01

    Six dimensional target information is produced by a target tracking vision system for use in real time target tracking by a robotic system. The vision system described in this paper produces three axes of position data and three axes of orientation data using a single camera which views a three dimensional target. The system performs target detection, target discrimination and determines the target position and orientation relative to the camera. As the target information is determined, it is communicated to a computer which is controlling the robot motion in real time. Details of the image processing algorithms and image processing hardware used in the vision system are discussed in the paper.

  12. Towards Real Time Diagnostics of Hybrid Welding Laser/GMAW

    Energy Technology Data Exchange (ETDEWEB)

    Timothy Mcjunkin; Dennis C. Kunerth; Corrie Nichol; Evgueni Todorov; Steve Levesque; Feng Yu; Robert Danna Couch

    2013-07-01

    Methods are currently being developed towards a more robust system real time feedback in the high throughput process combining laser welding with gas metal arc welding. A combination of ultrasonic, eddy current, electronic monitoring, and visual techniques are being applied to the welding process. Initial simulation and bench top evaluation of proposed real time techniques on weld samples are presented along with the concepts to apply the techniques concurrently to the weld process. Consideration for the eventual code acceptance of the methods and system are also being researched as a component of this project. The goal is to detect defects or precursors to defects and correct when possible during the weld process.

  13. Weak Serializable Concurrency Control in Real-time Broadcast Environments

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Given the asymmetric communication capabilities in mobile real-time environments, traditional serializability-based approaches are too restrictive, unnecessary, and impractical. Thus propose the use of a new weaker correctness criterion is proposed. This paper first formally defines the new notion of correctness called weak serializability. After the necessary and sufficient conditions for weak serializability are shown, corresponding concurrency control protocol based on this criterion is outlined for real-time broadcast environments. Finally, the simulation results confirmed that the proposed techniques help mobile transactions to meet their deadlines and improve query response time.

  14. A Contribution to Real-Time Experiments in Remote Laboratories

    Directory of Open Access Journals (Sweden)

    Zoltán Janík

    2013-02-01

    Full Text Available The paper is focused on realization of hard real-time control of experiments in on-line laboratories. The presented solution utilizes already developed on-line laboratory portal that is based on open-source Scilab environment. The customized solution is based on Linux RTAI platform with RTAI-XML server, Comedi and jRTAILab with support of ScicosLab environment. It generates real-time executable code that is used to operate student experiments performed on Humusoft CE152 Magnetic Levitation plant.

  15. Real-time Social Internet Data to Guide Forecasting Models

    Energy Technology Data Exchange (ETDEWEB)

    Del Valle, Sara Y. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-09-20

    Our goal is to improve decision support by monitoring and forecasting events using social media, mathematical models, and quantifying model uncertainty. Our approach is real-time, data-driven forecasts with quantified uncertainty: Not just for weather anymore. Information flow from human observations of events through an Internet system and classification algorithms is used to produce quantitatively uncertain forecast. In summary, we want to develop new tools to extract useful information from Internet data streams, develop new approaches to assimilate real-time information into predictive models, validate approaches by forecasting events, and our ultimate goal is to develop an event forecasting system using mathematical approaches and heterogeneous data streams.

  16. Real-time digital signal processing fundamentals, implementations and applications

    CERN Document Server

    Kuo, Sen M; Tian, Wenshun

    2013-01-01

    Combines both the DSP principles and real-time implementations and applications, and now updated with the new eZdsp USB Stick, which is very low cost, portable and widely employed at many DSP labs. Real-Time Digital Signal Processing introduces fundamental digital signal processing (DSP) principles and will be updated to include the latest DSP applications, introduce new software development tools and adjust the software design process to reflect the latest advances in the field. In the 3rd edition of the book, the key aspect of hands-on experiments will be enhanced to make the DSP principle

  17. Infrared Image Real-time Enhancement Based on DSP

    Institute of Scientific and Technical Information of China (English)

    DAI Shao-sheng; YUAN Xiang-hui; XUE Lian

    2004-01-01

    Since image real- time processing requires vast amount of computation and high- speed hardware,it is difficult to be implemented with general microcomputer system. In order to solve the problem,a powerful digital signal processing (DSP) hardware system is proposed,which is able to meet needs of image real-time processing. There are many approaches to enhance infrared image. But only histogram equalization is discussed because it is the most common and effective way. On the basis of histogram equalization principle, the specific procedures implemented in DSP are shown. At last the experimental results are given.

  18. Real-time medical collaboration services over the web.

    Science.gov (United States)

    Andrikos, Christos; Rassias, Georgios; Tsanakas, Panayiotis; Maglogiannis, Ilias

    2015-08-01

    The gradual shift in modern medical practice, from working alone clinical doctors to MDTs (Multi-Disciplinary Teams), raises the need of online real-time collaboration among geographically distributed medical personnel. The paper presents a Web-based platform, featuring an efficient medical data management and exchange, for hosting real-time collaborative services. The presented work leverages state-of-the-art features of the web (technologies and APIs) to support client-side medical data processing. Moreover, to address the typical bandwidth bottleneck and known scalability issues of centralized data sharing, an indirect RPC (Remote Process Call) scheme is introduced through object synchronization over the WebRTC paradigm.

  19. Requirements Specifications Checking of Embedded Real-Time Software

    Institute of Scientific and Technical Information of China (English)

    WU Guoqing(毋国庆); SHU Fengdi(舒风笛); WANG Min(王敏); CHEN Weiqing(陈伟清)

    2002-01-01

    After introducing the overview of our requirements description model HRFSM,the paper presents a dynamic software execution model (DERTS) of embedded real-time software, which can integrate control flow, data flow and time. Based on DERTS, a checking method is also presented. It consists of three kinds of checking and can check the consistency and completeness of the requirement specifications of embedded real-time software. Besides providing information helpful to improve the efficiency of analyzing and checking specifications,the checking method is flexible, and easy to understand and to use for the analyst.

  20. Comparative Proteomic Analysis of Two Barley Cultivars (Hordeum vulgare L.) with Contrasting Grain Protein Content

    Science.gov (United States)

    Guo, Baojian; Luan, Haiye; Lin, Shen; Lv, Chao; Zhang, Xinzhong; Xu, Rugen

    2016-01-01

    Grain protein contents (GPCs) of barley seeds are significantly different between feed and malting barley cultivars. However, there is still no insight into the proteomic analysis of seed proteins between feed and malting barley cultivars. Also, the genetic control of barley GPC is still unclear. GPCs were measured between mature grains of Yangsimai 3 and Naso Nijo. A proteome profiling of differentially expressed protein was established by using a combination of 2-DE and tandem mass spectrometry. In total, 502 reproducible protein spots in barley seed proteome were detected with a pH range of 4–7 and 6–11, among these 41 protein spots (8.17%) were detected differentially expressed between Yangsimai 3 and Naso Nijo. Thirty-four protein spots corresponding to 23 different proteins were identified, which were grouped into eight categories, including stress, protein degradation and post-translational modification, development, cell, signaling, glycolysis, starch metabolism, and other functions. Among the identified proteins, enolase (spot 274) and small subunit of ADP-glucose pyrophosphorylase (spot 271) are exclusively expressed in barley Yangsimai 3, which may be involved in regulating seed protein expression. In addition, malting quality is characterized by an accumulation of serpin protein, Alpha-amylase/trypsin inhibitor CMb and Alpha-amylase inhibitor BDAI-1. Most noticeably, globulin, an important storage protein in barley seed, undergoes post-translational processing in both cultivars, and also displays different expression patterns. PMID:27200019

  1. Real-time control of multiple MHD instabilities on TCV by ECRH/ECCD

    Directory of Open Access Journals (Sweden)

    Sauter O.

    2012-09-01

    Full Text Available Highly localized deposition of ECRH/ECCD is particularly suited for MHD control, in particular when combined with real-time beam orientation and power control capabilities. The powerful (4.5MW and flexible (7 steerable launcher EC system on TCV has recently been complemented by an equally flexible digital real-time control system with the aim of developing and testing integrated MHD control methods [1]. Sawtooth pacing is one such method [2]. The crash time of stabilized sawteeth can be precisely controled by removing the EC power at a given time after the last sawtooth crash, causing the crash to occur at a short and reproducible time thereafter. This control strategy is combined with efficient neoclassical tearing mode (NTM preemption by depositing power at the mode rational surfaces only during a short time synchronized with the island-seeding sawtooth crash. If an NTM appears nevertheless, full power is applied to stabilize the mode. The real-time steerable launchers have also been employed to stabilize fully saturated NTMs and to investigate the precise requirements for deposition localization for full island stabilization. Finally, though ELM dynamics is markedly different, recent results show that ELM pacing is possible using a similar control technique as used for sawtooth pacing. In this case, edge EC power is removed after each ELM, and is reapplied after a programmable time interval. The ELM period can be real-time controlled by adjusting the length of this interval. While the overall trend conforms to the increase of ELM frequency with increasing power, this technique provides a means to significantly regularize the ELM cycle.

  2. Interfacing Agents to Real-Time Strategy Games

    DEFF Research Database (Denmark)

    Jensen, Andreas Schmidt; Kaysø-Rørdam, Christian; Villadsen, Jørgen

    2015-01-01

    In real-time strategy games players make decisions and control their units simultaneously. Players are required to make decisions under time pressure and should be able to control multiple units at once in order to be successful. We present the design and implementation of a multi-agent interface...

  3. Hardware Approach for Real Time Machine Stereo Vision

    Directory of Open Access Journals (Sweden)

    Michael Tornow

    2006-02-01

    Full Text Available Image processing is an effective tool for the analysis of optical sensor information for driver assistance systems and controlling of autonomous robots. Algorithms for image processing are often very complex and costly in terms of computation. In robotics and driver assistance systems, real-time processing is necessary. Signal processing algorithms must often be drastically modified so they can be implemented in the hardware. This task is especially difficult for continuous real-time processing at high speeds. This article describes a hardware-software co-design for a multi-object position sensor based on a stereophotogrammetric measuring method. In order to cover a large measuring area, an optimized algorithm based on an image pyramid is implemented in an FPGA as a parallel hardware solution for depth map calculation. Object recognition and tracking are then executed in real-time in a processor with help of software. For this task a statistical cluster method is used. Stabilization of the tracking is realized through use of a Kalman filter. Keywords: stereophotogrammetry, hardware-software co-design, FPGA, 3-d image analysis, real-time, clustering and tracking.

  4. The Danish real-time SAR processor: first results

    DEFF Research Database (Denmark)

    Dall, Jørgen; Jørgensen, Jørn Hjelm; Netterstrøm, Anders;

    1993-01-01

    A real-time processor (RTP) for the Danish airborne Synthetic Aperture Radar (SAR) has been designed and constructed at the Electromagnetics Institute. The implementation was completed in mid 1992, and since then the RTP has been operated successfully on several test and demonstration flights...

  5. Semantics of the VDM Real-Time Dialect

    DEFF Research Database (Denmark)

    Lausdahl, Kenneth; Coleman, Joey; Larsen, Peter Gorm

    All formally defined languages need to be given an unambiguous semantics such that the meaning of all models expressed using the language is clear. In this technical report a semantic model is provided for the Real-Time dialect of the Vienna Development Method (VDM). This builds upon both...

  6. Real-time distortionless high-factor compression scheme.

    Science.gov (United States)

    Liénard, J

    1989-01-01

    Nowadays, digital subtraction angiography systems must be able to sustain real-time acquisition (30 frames per second) of 512 x 512 x 8 bit images and store several sequences of such images on low cost and general-purpose mass memories. Concretely, that means a 7.8 Mbytes per second rate and about 780 Mbytes disk space to hold a 100-s cardiac examination. To fulfill these requirements at competitive cost, a distortionless compressor/decompressor system can be designed: during acquisition, the real-time compressor transforms the input images into a lower quantity of coded information through a predictive coder and a variable-length Huffman code. The process is fully reversible because during review, the real-time decompressor exactly recovers the acquired images from the stored compressed data. Test results on many raw images demonstrate that real-time compression is feasible and takes place with absolutely no loss of information. The designed system indifferently works on 512 or 1024 formats, and 256 or 1024 gray levels.

  7. Emergency Department Real Time Location System Patient and Equipment Tracking

    Science.gov (United States)

    2014-10-01

    identify key opportunities to drive operational efficiency, improve patient satisfaction , and increase asset utilization. The project will also focus on...operative setting tagging other assets as well as patients and caregivers. Body Background: The Valley Hospital proposes to implement a real time...area to identify key opportunities to drive operational efficiency, improve patient satisfaction , and increase asset utilization. The pilot project

  8. Testing power system controllers by real-time simulation

    DEFF Research Database (Denmark)

    Venne, Philippe; Guillaud, Xavier; Sirois, Frédéric

    2007-01-01

    In this paper, we present a number of state-of-the art methods for testing power system controllers based on the use of a real-time power system simulator. After introducing Hypersim, we list and discuss the different means of connection between the controller under tests and the power system...

  9. Real-Time GNSS Positioning Along Canada's Active Coastal Margin

    Science.gov (United States)

    Henton, J. A.; Dragert, H.; Lu, Y.

    2014-12-01

    High-rate, low-latency Global Navigation Satellite System (GNSS) data are being refined for real-time applications to monitor and report motions related to large earthquakes in coastal British Columbia. Given the tectonic setting of Canada's west coast, specific goals for real-time regional geodetic monitoring are: (1) the collection of GNSS data with adequate station density to identify the deformation field for regional earthquakes with M>7.3; (2) the robust, continuous real-time analyses of GNSS data with a precision of 1-2 cm and a latency of less than 10s; and (3) the display of results with attending automated alarms and estimations of earthquake parameters. Megathrust earthquakes (M>8) are the primary targets for immediate identification, since the tsunamis they generate will strike the coast within 15 to 20 min. However, large (6.0displacements expected from various offshore events which allows an evaluation of the effectiveness of the current regional coverage. The present distribution and density of real-time sites is largely sufficient for aiding the timely estimation of size, location, and nature of a great (M>8) megathrust earthquake. However, current coverage is inadequate for the unambiguous identification of the same parameters for 7

  10. Real-time vehicle emission monitoring along roadsides

    NARCIS (Netherlands)

    Stelwagen, U.; Lange, R. de; Ligterink, N.E.; Klunder, G.A.; Bigazzi, A.; Duyzer, J.H.; Baalen, J. van; Katwijk, R.T. van; Kruithof, M.C.; Ratingen, S. van; Weststrate, J.H.; Wilmink, I.R.

    2010-01-01

    In the worldwide efforts to reduce the emissions of greenhouse gasses in general and those emitted by vehicles in particular, vehicle emission monitoring is important. It provides accurate knowledge of real-world emissions of vehicles as input for vehicle emission models. Real-time emission monitori

  11. Real-time speech gisting for ATC applications

    Science.gov (United States)

    Dunkelberger, Kirk A.

    1995-06-01

    Command and control within the ATC environment remains primarily voice-based. Hence, automatic real time, speaker independent, continuous speech recognition (CSR) has many obvious applications and implied benefits to the ATC community: automated target tagging, aircraft compliance monitoring, controller training, automatic alarm disabling, display management, and many others. However, while current state-of-the-art CSR systems provide upwards of 98% word accuracy in laboratory environments, recent low-intrusion experiments in the ATCT environments demonstrated less than 70% word accuracy in spite of significant investments in recognizer tuning. Acoustic channel irregularities and controller/pilot grammar verities impact current CSR algorithms at their weakest points. It will be shown herein, however, that real time context- and environment-sensitive gisting can provide key command phrase recognition rates of greater than 95% using the same low-intrusion approach. The combination of real time inexact syntactic pattern recognition techniques and a tight integration of CSR, gisting, and ATC database accessor system components is the key to these high phase recognition rates. A system concept for real time gisting in the ATC context is presented herein. After establishing an application context, discussion presents a minimal CSR technology context then focuses on the gisting mechanism, desirable interfaces into the ATCT database environment, and data and control flow within the prototype system. Results of recent tests for a subset of the functionality are presented together with suggestions for further research.

  12. Real time Aanderaa current meter data collection system

    Digital Repository Service at National Institute of Oceanography (India)

    AshokKumar, K.; Diwan, S.G.

    in laboratory. In this paper a method is described to read the real time current meter data and display/print/store on cartridge. For this, binary coded electrical signal available at the top end plate of the current meter is connectEd. by underwater cable...

  13. Hardware Algorithms For Tile-Based Real-Time Rendering

    NARCIS (Netherlands)

    Crisu, D.

    2012-01-01

    In this dissertation, we present the GRAphics AcceLerator (GRAAL) framework for developing embedded tile-based rasterization hardware for mobile devices, meant to accelerate real-time 3-D graphics (OpenGL compliant) applications. The goal of the framework is a low-cost, low-power, high-performance d

  14. Wireless Sensor Network Metrics for Real-Time Systems

    Science.gov (United States)

    2009-05-20

    time measurement system . . . . . . . . . . . . . . . . . . . . . . . . . 4 1.2 Real-time networked control system . . . . . . . . . . . . . . . . . . . . . . 5...topology with GMM2 and APLM . . . . . . . . . . 103 4.1 Networked control system . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106 4.2 One / two...4.1. The key issue in designing a wireless networked control system is addressing how to guarantee system performance and stability when the

  15. Rattlesnake: a network for real-time Multimedia Communication

    NARCIS (Netherlands)

    Smit, Gerard J.M.; Havinga, Paul J.M.; Smit, Michel J.P.

    1992-01-01

    In this extended abstract we describe the design of a local area network suitable for distributed multimedia communications. Multimedia applications require a communication infrastructure with capabilities beyond the current state of the art : real-time stream traffic, small end-to-end latency with

  16. Rattlesnake: a Network for Real-time Multimedia Communication

    NARCIS (Netherlands)

    Smit, Gerard J.M.; Havinga, Paul J.M.; Smit, Michel J.P.

    1992-01-01

    In this extended abstract we describe the design of a local area network suitable for distributed multimedia communications. Multimedia applications require a communication infrastructure with capabilities beyond the current state of the art : real-time stream traffic, small end-to-end latency with

  17. Robust regression methods for real-time polymerase chain reaction

    NARCIS (Netherlands)

    Trypsteen, Wim; De Neve, Jan; Bosman, Kobus; Nijhuis, Monique; Thas, Olivier; Vandekerckhove, Linos; De Spiegelaere, Ward

    2015-01-01

    Current real-time polymerase chain reaction (PCR) data analysis methods implement linear least squares regression methods for primer efficiency estimation based on standard curve dilution series. This method is sensitive to outliers that distort the outcome and are often ignored or removed by the en

  18. Introducing Undergraduate Students to Real-Time PCR

    Science.gov (United States)

    Hancock, Dale; Funnell, Alister; Jack, Briony; Johnston, Jill

    2010-01-01

    An experiment is conducted, which in four 3 h laboratory sessions, introduces third year undergraduate Biochemistry students to the technique of real-time PCR in a biological context. The model used is a murine erythroleukemia cell line (MEL cells). These continuously cycling, immature red blood cells, arrested at an early stage in erythropoiesis,…

  19. Real time traffic models, decision support for traffic management

    NARCIS (Netherlands)

    Wismans, L.J.J.; Romph, de E.; Friso, K.; Zantema, K.

    2014-01-01

    Reliable and accurate short-term traffic state prediction can improve the performance of real-time traffic management systems significantly. Using this short-time prediction based on current measurements delivered by advanced surveillance systems will support decision-making processes on various con

  20. Real Time Traffic Models, Decision Support for Traffic Management

    NARCIS (Netherlands)

    Wismans, L.; De Romph, E.; Friso, K.; Zantema, K.

    2014-01-01

    Reliable and accurate short-term traffic state prediction can improve the performance of real-time traffic management systems significantly. Using this short-time prediction based on current measurements delivered by advanced surveillance systems will support decision-making processes on various con

  1. HAMLET: HPCN Technology for Real-Time, Embedded Applications

    NARCIS (Netherlands)

    Mager, J.W.L.J.; Dam, A. ten

    1995-01-01

    Building an application by using HPCN technology makes the solution scalable and therefore more flexible. The uptake of HPCN technology for real-time, embedded applications, however, is severely hampered by the lack of real application development support. Within the Esprit project HAMLET, technolog

  2. Knowledge exchange between agents in real-time environments

    DEFF Research Database (Denmark)

    Frisvad, Jeppe Revall; Falster, Peter; Møller, Gert Lykke

    2005-01-01

    To obtain unpredictable social interaction between autonomous agents in real-time environments, we present a simple method for logic-based knowledge exchange. A method which is able to form new knowledge rather than do simple exchange of particular rules found in predetermined rule sets. The appl...

  3. Real Time Surface Registration for PET Motion Tracking

    DEFF Research Database (Denmark)

    Wilm, Jakob; Olesen, Oline Vinter; Paulsen, Rasmus Reinhold

    2011-01-01

    to create point clouds representing parts of the patient's face. The movement is estimated by a rigid registration of the point clouds. The registration should be done using a robust algorithm that can handle partial overlap and ideally operate in real time. We present an optimized Iterative Closest Point...

  4. SARUS: A Synthetic Aperture Real-Time Ultrasound System

    DEFF Research Database (Denmark)

    Jensen, Jørgen Arendt; Holten-Lund, Hans; Nilsson, Ronnie Thorup

    2013-01-01

    The Synthetic Aperture Real-time Ultrasound System (SARUS) for acquiring and processing synthetic aperture (SA) data for research purposes is described. The specifications and design of the system are detailed, along with its performance for SA, nonlinear, and 3-D flow estimation imaging. SARUS...

  5. Real-Time Visualization of Large Textured Terrains

    DEFF Research Database (Denmark)

    Brodersen, Anders Torp

    2005-01-01

    In this paper, we present a framework for real-time rendering of large scale terrains with texture maps larger than what the graphics hardware can display in a single texture. The presented system is compact and efficient, yet very simple and easy to implement....

  6. Hierarchical Real-time Network Traffic Classification Based on ECOC

    Directory of Open Access Journals (Sweden)

    Yaou Zhao

    2013-09-01

    Full Text Available Classification of network traffic is basic and essential for manynetwork researches and managements. With the rapid development ofpeer-to-peer (P2P application using dynamic port disguisingtechniques and encryption to avoid detection, port-based and simplepayload-based network traffic classification methods were diminished.An alternative method based on statistics and machine learning hadattracted researchers' attention in recent years. However, most ofthe proposed algorithms were off-line and usually used a single classifier.In this paper a new hierarchical real-time model was proposed which comprised of a three tuple (source ip, destination ip and destination portlook up table(TT-LUT part and layered milestone part. TT-LUT was used to quickly classify short flows whichneed not to pass the layered milestone part, and milestones in layered milestone partcould classify the other flows in real-time with the real-time feature selection and statistics.Every milestone was a ECOC(Error-Correcting Output Codes based model which was usedto improve classification performance. Experiments showed that the proposedmodel can improve the efficiency of real-time to 80%, and themulti-class classification accuracy encouragingly to 91.4% on the datasets which had been captured from the backbone router in our campus through a week.

  7. Detection of magnetising inrush current using real time integration method

    Science.gov (United States)

    Ling, P. C. Y.; Basak, A.

    1990-01-01

    A technique of predicting magnetising inrush currents in transformers is described. Computed results show an inconsistency in second harmonic decay resulting detection failure while using conventional second harmonic techniques. A new detection scheme using real time integration values of the inrush current is proposed to provide reliable relay operation.

  8. Learning in Real-Time Search: A Unifying Framework

    CERN Document Server

    Bulitko, V; 10.1613/jair.1789

    2011-01-01

    Real-time search methods are suited for tasks in which the agent is interacting with an initially unknown environment in real time. In such simultaneous planning and learning problems, the agent has to select its actions in a limited amount of time, while sensing only a local part of the environment centered at the agents current location. Real-time heuristic search agents select actions using a limited lookahead search and evaluating the frontier states with a heuristic function. Over repeated experiences, they refine heuristic values of states to avoid infinite loops and to converge to better solutions. The wide spread of such settings in autonomous software and hardware agents has led to an explosion of real-time search algorithms over the last two decades. Not only is a potential user confronted with a hodgepodge of algorithms, but he also faces the choice of control parameters they use. In this paper we address both problems. The first contribution is an introduction of a simple three-parameter framework...

  9. Real-time change detection in data streams with FPGAs

    Energy Technology Data Exchange (ETDEWEB)

    Vega, J., E-mail: jesus.vega@ciemat.es [Asociación EURATOM/CIEMAT para Fusión, Avda. Complutense, 22, 28040 Madrid (Spain); Dormido-Canto, S.; Cruz, T. [Departamento de Informática y Automática, UNED, Madrid (Spain); Ruiz, M.; Barrera, E. [Grupo de Investigación en Instrumentación y Acústica Aplicada, Universidad Politécnica de Madrid, Madrid (Spain); Castro, R. [Asociación EURATOM/CIEMAT para Fusión, Avda. Complutense, 22, 28040 Madrid (Spain); Murari, A. [Associazione EURATOM-ENEA per la Fusione, Consorzio RFX, I-35127 Padova (Italy); Ochando, M. [Asociación EURATOM/CIEMAT para Fusión, Avda. Complutense, 22, 28040 Madrid (Spain)

    2014-05-15

    Highlights: • Automatic recognition of changes in data streams of multidimensional signals. • Detection algorithm based on testing exchangeability on-line. • Real-time and off-line applicability. • Real-time implementation in FPGAs. - Abstract: The automatic recognition of changes in data streams is useful in both real-time and off-line data analyses. This article shows several effective change-detecting algorithms (based on martingales) and describes their real-time applicability in the data acquisition systems through the use of Field Programmable Gate Arrays (FPGA). The automatic event recognition system is absolutely general and it does not depend on either the particular event to detect or the specific data representation (waveforms, images or multidimensional signals). The developed approach provides good results for change detection in both the temporal evolution of profiles and the two-dimensional spatial distribution of volume emission intensity. The average computation time in the FPGA is 210 μs per profile.

  10. Real-Time Analytics for the Healthcare Industry: Arrhythmia Detection.

    Science.gov (United States)

    Agneeswaran, Vijay Srinivas; Mukherjee, Joydeb; Gupta, Ashutosh; Tonpay, Pranay; Tiwari, Jayati; Agarwal, Nitin

    2013-09-01

    It is time for the healthcare industry to move from the era of "analyzing our health history" to the age of "managing the future of our health." In this article, we illustrate the importance of real-time analytics across the healthcare industry by providing a generic mechanism to reengineer traditional analytics expressed in the R programming language into Storm-based real-time analytics code. This is a powerful abstraction, since most data scientists use R to write the analytics and are not clear on how to make the data work in real-time and on high-velocity data. Our paper focuses on the applications necessary to a healthcare analytics scenario, specifically focusing on the importance of electrocardiogram (ECG) monitoring. A physician can use our framework to compare ECG reports by categorization and consequently detect Arrhythmia. The framework can read the ECG signals and uses a machine learning-based categorizer that runs within a Storm environment to compare different ECG signals. The paper also presents some performance studies of the framework to illustrate the throughput and accuracy trade-off in real-time analytics.

  11. Nonlinear projective filtering; 1, Application to real time series

    CERN Document Server

    Schreiber, T

    1998-01-01

    We discuss applications of nonlinear filtering of time series by locally linear phase space projections. Noise can be reduced whenever the error due to the manifold approximation is smaller than the noise in the system. Examples include the real time extraction of the fetal electrocardiogram from abdominal recordings.

  12. Smart Shopping Carts: How Real-Time Feedback Influences Spending

    NARCIS (Netherlands)

    Ittersum, van K.; Wansink, B.; Pennings, J.M.E.; Sheehan, D.

    2013-01-01

    Although interest in smart shopping carts is increasing, both retailers and consumer groups have concerns about how real-time spending feedback will influence shopping behavior. Building on budgeting and spending theories, the authors conduct three lab and grocery store experiments that robustly sho

  13. Smart shopping carts : How real-time feedback influences spending

    NARCIS (Netherlands)

    van Ittersum, Koert; Wansink, B.; Pennings, J.M.E.; Sheehan, D.

    2013-01-01

    Although interest in smart shopping carts is increasing, both retailers and consumer groups have concerns about how real-time spending feedback will influence shopping behavior. Building on budgeting and spending theories, the authors conduct three lab and grocery store experiments that robustly sho

  14. DSP Based System for Real time Voice Synthesis Applications Development

    CERN Document Server

    Arsinte, Radu; Miron, Costin

    2008-01-01

    This paper describes an experimental system designed for development of real time voice synthesis applications. The system is composed from a DSP coprocessor card, equipped with an TMS320C25 or TMS320C50 chip, voice acquisition module (ADDA2),host computer (IBM-PC compatible), software specific tools.

  15. Real-time PCR: Advanced technologies and applications

    Science.gov (United States)

    This book brings together contributions from 20 experts in the field of PCR, providing a broad perspective of the applications of quantitative real-time PCR (qPCR). The editors state in the preface that the aim is to provide detailed insight into underlying principles and methods of qPCR to provide ...

  16. Real-Time Capture of Student Reasoning While Writing

    Science.gov (United States)

    Franklin, Scott V.; Hermsen, Lisa M.

    2014-01-01

    We present a new approach to investigating student reasoning while writing: real-time capture of the dynamics of the writing process. Key-capture or video software is used to record the entire writing episode, including all pauses, deletions, insertions, and revisions. A succinct shorthand, "S notation," is used to highlight significant…

  17. Real-Time Ocean Data in the Classroom

    Science.gov (United States)

    Murray, Laura; Gibson, Deidre; Ward, Angela

    2008-01-01

    To apply students' savvy internet skills in the science classroom--as well as capture their interest in science and investigation, and provide opportunities for authentic research--introduce them to real-time data from ocean-observing systems. Students can use data from these ocean-observing systems to discover the winds and waves from storms or…

  18. In Vivo Real Time Volumetric Synthetic Aperture Ultrasound Imaging

    DEFF Research Database (Denmark)

    Bouzari, Hamed; Rasmussen, Morten Fischer; Brandt, Andreas Hjelm;

    2015-01-01

    Synthetic aperture (SA) imaging can be used to achieve real-time volumetric ultrasound imaging using 2-D array transducers. The sensitivity of SA imaging is improved by maximizing the acoustic output, but one must consider the limitations of an ultrasound system, both technical and biological...

  19. Real-time image fusion involving diagnostic ultrasound

    DEFF Research Database (Denmark)

    Ewertsen, Caroline; Săftoiu, Adrian; Gruionu, Lucian G;

    2013-01-01

    The aim of our article is to give an overview of the current and future possibilities of real-time image fusion involving ultrasound. We present a review of the existing English-language peer-reviewed literature assessing this technique, which covers technical solutions (for ultrasound...... and endoscopic ultrasound), image fusion in several anatomic regions, and electromagnetic needle tracking....

  20. Real-time hyperspectral processing for automatic nonferrous material sorting

    Science.gov (United States)

    Picón, Artzai; Ghita, Ovidiu; Bereciartua, Aranzazu; Echazarra, Jone; Whelan, Paul F.; Iriondo, Pedro M.

    2012-01-01

    The application of hyperspectral sensors in the development of machine vision solutions has become increasingly popular as the spectral characteristics of the imaged materials are better modeled in the hyperspectral domain than in the standard trichromatic red, green, blue data. While there is no doubt that the availability of detailed spectral information is opportune as it opens the possibility to construct robust image descriptors, it also raises a substantial challenge when this high-dimensional data is used in the development of real-time machine vision systems. To alleviate the computational demand, often decorrelation techniques are commonly applied prior to feature extraction. While this approach has reduced to some extent the size of the spectral descriptor, data decorrelation alone proved insufficient in attaining real-time classification. This fact is particularly apparent when pixel-wise image descriptors are not sufficiently robust to model the spectral characteristics of the imaged materials, a case when the spatial information (or textural properties) also has to be included in the classification process. The integration of spectral and spatial information entails a substantial computational cost, and as a result the prospects of real-time operation for the developed machine vision system are compromised. To answer this requirement, in this paper we have reengineered the approach behind the integration of the spectral and spatial information in the material classification process to allow the real-time sorting of the nonferrous fractions that are contained in the waste of electric and electronic equipment scrap.

  1. Model-Checking Real-Time Control Programs

    DEFF Research Database (Denmark)

    Iversen, T. K.; Kristoffersen, K. J.; Larsen, Kim Guldstrand

    2000-01-01

    In this paper, we present a method for automatic verification of real-time control programs running on LEGO(R) RCX(TM) bricks using the verification tool UPPALL. The control programs, consisting of a number of tasks running concurrently, are automatically translated into the mixed automata model...

  2. ADA and multi-microprocessor real-time simulation

    Science.gov (United States)

    Feyock, S.; Collins, W. R.

    1983-01-01

    The selection of a high-order programming language for a real-time distributed network simulation is described. The additional problem of implementing a language on a possibly changing network is addressed. The recently designed language ADA (trademarked by DoD) was chosen since it provides the best model of the underlying application to be simulated.

  3. Real-Time PCR Quantification of Methanobrevibacter oralis in Periodontitis

    OpenAIRE

    Bringuier, Amélie; Khelaifia, Saber; Richet, Hervé; Aboudharam, Gérard; Drancourt, Michel

    2013-01-01

    A real-time PCR assay developed to quantify Methanobrevibacter oralis indicated that its inoculum significantly correlated with periodontitis severity (P = 0.003), despite a nonsignificant difference in prevalence between controls (3/10) and patients (12/22) (P = 0.2, Fisher test). The M. oralis load can be used as a biomarker for periodontitis.

  4. Emergency Department Real Time Location System Patient and Equipment Tracking

    Science.gov (United States)

    2013-10-01

    solution complies with all RF transmission guidelines to avoid interference with Hospital’s wireless communication systems and clinical...technology path that the messages will follow is, AgileTrac ESB services will capture real time updates of patient locations and push that

  5. Diagnosing herpesvirus infections by real time amplification and rapid culture.

    NARCIS (Netherlands)

    J. Guldemeester; A.D.M.E. Osterhaus (Albert); H.G.M. Niesters (Bert); G.J.J. van Doornum (Gerard)

    2003-01-01

    textabstractProcedures using real-time technique were developed to demonstrate the presence of herpes simplex virus type 1 (HSV-1) and HSV-2, varicella zoster virus (VZV), and cytomegalovirus (CMV) in miscellaneous clinical specimens. The assays were compared to rapid culture using centrifugation fo

  6. Real Time Scheduling Services for Distributed RT-CORBA Applications

    Directory of Open Access Journals (Sweden)

    Bineta Tresa Mathew

    2012-12-01

    Full Text Available Distributed computing environment is flexible to control in complex embedded systems and their software components gain complexity when these systems are equipped with many microcontrollers and software object which covers diverse platforms, this system is called as DRE system. These DRE systems need new inter-object communication solution thus QoS-enabled middleware services and mechanisms have begun to emerge. Real-time application domain benefit from flexible and open distributed architectures, such as those defined by the CORBA specification. CORBA is well-suited to conventional request/response applications, but not suited to real-time applications due to the lack of QoS features and performance optimizations. The paper shows the design and implementation of the high performance scheduling technique for the real time applications domain with CORBA systems. Four different algorithms are compared by using attributes of real time tasks constraints based on CORBA specification such as RMS, MLF, MUF and EDF. The experimental outcome demonstrates the better performance of MLF by analyzing the time taken for the execution of several numbers of tasks and further it can be compared with the combination of RMS and MLF to reach the best performance strategy.

  7. Real time process algebra with time-dependent conditions

    NARCIS (Netherlands)

    Baeten, J.C.M.; Middelburg, C.A.

    2008-01-01

    We extend the main real time version of ACP presented in [6] with conditionals in which the condition depends on time. This extension facilitates flexible dependence of proccess behaviour on initialization time. We show that the conditions concerned generalize the conditions introduced earlier in a

  8. Representing real time semantics for distributed application integration

    NARCIS (Netherlands)

    Poon, P.M.S.; Dillon, T.S.; Chang, E.; Feng, L.

    2006-01-01

    Traditional real time system design and development are driven by technological requirements. With the ever growing complexity of requirements and the advances in software design, the alignment of focus has gradually been shifted to the perspective of business and industrial needs. This paper discus

  9. Real-time measurements of jet aircraft engine exhaust.

    Science.gov (United States)

    Rogers, Fred; Arnott, Pat; Zielinska, Barbara; Sagebiel, John; Kelly, Kerry E; Wagner, David; Lighty, JoAnn S; Sarofim, Adel F

    2005-05-01

    Particulate-phase exhaust properties from two different types of ground-based jet aircraft engines--high-thrust and turboshaft--were studied with real-time instruments on a portable pallet and additional time-integrated sampling devices. The real-time instruments successfully characterized rapidly changing particulate mass, light absorption, and polycyclic aromatic hydrocarbon (PAH) content. The integrated measurements included particulate-size distributions, PAH, and carbon concentrations for an entire test run (i.e., "run-integrated" measurements). In all cases, the particle-size distributions showed single modes peaking at 20-40nm diameter. Measurements of exhaust from high-thrust F404 engines showed relatively low-light absorption compared with exhaust from a turboshaft engine. Particulate-phase PAH measurements generally varied in phase with both net particulate mass and with light-absorbing particulate concentrations. Unexplained response behavior sometimes occurred with the real-time PAH analyzer, although on average the real-time and integrated PAH methods agreed within the same order of magnitude found in earlier investigations.

  10. Real-time Loudspeaker Distance Estimation with Stereo Audio

    DEFF Research Database (Denmark)

    Nielsen, Jesper Kjær; Gaubitch, Nikolay; Heusdens, Richard;

    2015-01-01

    . In this paper, we propose to use the desired audio signal instead. Specifically, we treat the case of estimating the distance between two loudspeakers playing back a stereo music or speech signal. In this connection, we develop a real-time maximum likelihood estimator and demonstrate that it has a variance...

  11. Capturing Real-Time Data in Disaster Response Logistics

    Directory of Open Access Journals (Sweden)

    Kezban Yagci Sokat

    2016-06-01

    Full Text Available The volume, accuracy, accessibility and level of detail of near real-time data emerging from disaster-affected regions continue to significantly improve. Integration of dynamically evolving in-field data is an important, yet often overlooked, component of the humanitarian logistics models. In this paper, we present a framework for real-time humanitarian logistics data focused on use in mathematical modeling along with modeling implications of this framework. We also discuss how one might measure the attributes of the framework and describe the application of the presented framework to a case study of near real-time data collection in the days following the landfall of Typhoon Haiyan. We detail our first-hand experience of capturing data as the post-disaster response unfolds starting on November 10, 2013 until March 31, 2014 and assess the characteristics and evolution of data pertaining to humanitarian logistics modeling using the proposed framework. The presented logistical content analysis examines the availability of data and informs modelers about the current state of near real-time data. This analysis illustrates what data is available, how early it is available, and how data changes after the disaster. The study describes how our humanitarian logistics team approached the emergence of dynamic online data after the disaster and the challenges faced during the collection process, as well as recommendations to address these challenges in the future (when possible from an academic humanitarian logistics perspective.

  12. A Semantics for a Real-Time Actor Language

    DEFF Research Database (Denmark)

    Knoll, Istvan; Ravn, Anders Peter; Skou, Arne

    2008-01-01

    In order to develop simulators and analysis tools for an actor based real-time language, we define its semantics. The semantics is interesting in itself, as it models the functional, communication, and timing aspects separately, allowing several variants of the language to be investigated....

  13. Quantitative Microplate Assay for Real-Time Nuclease Kinetics

    OpenAIRE

    Eriksson, Jonas; Langel, Ülo

    2016-01-01

    Utilizing the phenomenon of nucleases exposing oligonucleotide phosphate backbones to phosphatases we present a novel quantitative method for kinetics of nuclease catalysis. Inorganic phosphate released from nuclease products by phosphatases could be quantified in real-time by a fluorescent sensor of inorganic phosphate. Two different nucleases were employed, showing the versatility of this assay for multiple turnover label-free nuclease studies.

  14. Real-Time Attitude Independent Three Axis Magnetometer Calibration

    Science.gov (United States)

    Crassidis, John L.; Lai, Kok-Lam; Harman, Richard R.

    2003-01-01

    In this paper new real-time approaches for three-axis magnetometer sensor calibration are derived. These approaches rely on a conversion of the magnetometer-body and geomagnetic-reference vectors into an attitude independent observation by using scalar checking. The goal of the full calibration problem involves the determination of the magnetometer bias vector, scale factors and non-orthogonality corrections. Although the actual solution to this full calibration problem involves the minimization of a quartic loss function, the problem can be converted into a quadratic loss function by a centering approximation. This leads to a simple batch linear least squares solution. In this paper we develop alternative real-time algorithms based on both the extended Kalman filter and Unscented filter. With these real-time algorithms, a full magnetometer calibration can now be performed on-orbit during typical spacecraft mission-mode operations. Simulation results indicate that both algorithms provide accurate integer resolution in real time, but the Unscented filter is more robust to large initial condition errors than the extended Kalman filter. The algorithms are also tested using actual data from the Transition Region and Coronal Explorer (TRACE).

  15. Real-Time Motion Management of Prostate Cancer Radiotherapy

    DEFF Research Database (Denmark)

    Pommer, Tobias

    of this thesis is to manage prostate motion in real-time by aligning the radiation beam to the prostate using the novel dynamic multileaf collimator (DMLC) tracking method. Specifically, the delivered dose with tracking was compared to the planned dose, and the impact of treatment plan complexity and limitations...

  16. Real-time Navigator Approach to Motion Problems

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    @@ This presentation attempts a summary discourse on the real time navigator approach to suppressing motion effects in thoracic MRA, particularly in coronary MRA. Organs in the thoracic body trunk experience physiologic motion due to cardiac contraction, respiration, peristalsis and other gross displacements.

  17. Real-time analytics with Storm and Cassandra

    CERN Document Server

    Saxena, Shilpi

    2015-01-01

    If you want to efficiently use Storm and Cassandra together and excel at developing production-grade, distributed real-time applications, then this book is for you. No prior knowledge of using Storm and Cassandra together is necessary. However, a background in Java is expected.

  18. The Real-Time Corporate University Becomes a Reality.

    Science.gov (United States)

    Dealtry, Richard

    2002-01-01

    Traces the evolution of the corporate university, which combines traditional management tools with new learning process models, bridging strategic theory and real-time learning. Advocates the need for movement away from traditional cognitive paradigms, suggesting the use of image simulations and metamanagement thinking. (SK)

  19. Tera-sample-per-second Real-time Waveform Digitizer

    CERN Document Server

    Han, Y; Jalali, B; Han, Yan; Boyraz, Ozdal; Jalali, Bahram

    2005-01-01

    We demonstrate a real-time transient waveform digitizer with a record 1 TSa/s (Tera-Sample/sec) sampling rate. This is accomplished by using a photonic time stretch preprocessor which slows down the electrical waveform before it is captured by an electronic digitizer.

  20. A new real-time tsunami detection algorithm

    Science.gov (United States)

    Chierici, Francesco; Embriaco, Davide; Pignagnoli, Luca

    2017-01-01

    Real-time tsunami detection algorithms play a key role in any Tsunami Early Warning System. We have developed a new algorithm for tsunami detection based on the real-time tide removal and real-time band-pass filtering of seabed pressure recordings. The algorithm greatly increases the tsunami detection probability, shortens the detection delay and enhances detection reliability with respect to the most widely used tsunami detection algorithm, while containing the computational cost. The algorithm is designed to be used also in autonomous early warning systems with a set of input parameters and procedures which can be reconfigured in real time. We have also developed a methodology based on Monte Carlo simulations to test the tsunami detection algorithms. The algorithm performance is estimated by defining and evaluating statistical parameters, namely the detection probability, the detection delay, which are functions of the tsunami amplitude and wavelength, and the occurring rate of false alarms. Pressure data sets acquired by Bottom Pressure Recorders in different locations and environmental conditions have been used in order to consider real working scenarios in the test. We also present an application of the algorithm to the tsunami event which occurred at Haida Gwaii on 28 October 2012 using data recorded by the Bullseye underwater node of Ocean Networks Canada. The algorithm successfully ran for test purpose in year-long missions onboard abyssal observatories, deployed in the Gulf of Cadiz and in the Western Ionian Sea.

  1. Real-time PCR for Strongyloides stercoralis-associated meningitis.

    Science.gov (United States)

    Nadir, Eyal; Grossman, Tamar; Ciobotaro, Pnina; Attali, Malka; Barkan, Daniel; Bardenstein, Rita; Zimhony, Oren

    2016-03-01

    Four immunocompromised patients, immigrants from Ethiopia, presented with diverse clinical manifestations of meningitis associated with Strongyloides stercoralis dissemination as determined by identification of intestinal larvae. The cerebrospinal fluid of 3 patients was tested by a validated (for stool) real-time PCR for S. stercoralis and was found positive, establishing this association.

  2. Real-time in vivo photoacoustic and ultrasound imaging

    NARCIS (Netherlands)

    Kolkman, Roy G.M.; Brands, Peter J.; Steenbergen, Wiendelt; Leeuwen, van Ton G.

    2008-01-01

    A real-time photoacoustic imaging system is designed and built. This system is based on a commercially available ultrasound imaging system. It can achieve a frame rate of 8 frames/sec. Vasculature in the hand of a human volunteer is imaged, and the resulting photoacoustic image is combined with the

  3. Real-time depth map manipulation for 3D visualization

    Science.gov (United States)

    Ideses, Ianir; Fishbain, Barak; Yaroslavsky, Leonid

    2009-02-01

    One of the key aspects of 3D visualization is computation of depth maps. Depth maps enables synthesis of 3D video from 2D video and use of multi-view displays. Depth maps can be acquired in several ways. One method is to measure the real 3D properties of the scene objects. Other methods rely on using two cameras and computing the correspondence for each pixel. Once a depth map is acquired for every frame, it can be used to construct its artificial stereo pair. There are many known methods for computing the optical flow between adjacent video frames. The drawback of these methods is that they require extensive computation power and are not very well suited to high quality real-time 3D rendering. One efficient method for computing depth maps is extraction of motion vector information from standard video encoders. In this paper we present methods to improve the 3D visualization quality acquired from compression CODECS by spatial/temporal and logical operations and manipulations. We show how an efficient real time implementation of spatial-temporal local order statistics such as median and local adaptive filtering in 3D-DCT domain can substantially improve the quality of depth maps and consequently 3D video while retaining real-time rendering. Real-time performance is achived by utilizing multi-core technology using standard parallelization algorithms and libraries (OpenMP, IPP).

  4. Real-time optoacoustic monitoring of temperature in tissues

    Energy Technology Data Exchange (ETDEWEB)

    Larina, Irina V [Center for Biomedical Engineering, University of Texas Medical Branch, Galveston, TX 77555-0456 (United States); Department of Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX 77555-0456 (United States); Larin, Kirill V [Center for Biomedical Engineering, University of Texas Medical Branch, Galveston, TX 77555-0456 (United States); Esenaliev, Rinat O [Center for Biomedical Engineering, University of Texas Medical Branch, Galveston, TX 77555-0456 (United States); Department of Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX 77555-0456 (United States); Department of Anesthesiology, University of Texas Medical Branch, Galveston, TX 77555-0456 (United States)

    2005-08-07

    To improve the safety and efficacy of thermal therapy, it is necessary to map tissue temperature in real time with submillimetre spatial resolution. Accurate temperature maps may provide the necessary control of the boundaries of the heated regions and minimize thermal damage to surrounding normal tissues. Current imaging modalities fail to monitor tissue temperature in real time with high resolution and accuracy. We investigated a non-invasive optoacoustic method for accurate, real-time monitoring of tissue temperature during thermotherapy. In this study, we induced temperature gradients in tissue and tissue-like samples and monitored the temperature distribution using the optoacoustic technique. The fundamental harmonic of a Q-switched Nd : YAG laser ({lambda} = 1064 nm) was used for optoacoustic wave generation and probing of tissue temperature. The tissue temperature was also monitored with a multi-sensor temperature probe inserted in the samples. Good agreement between optoacoustically measured and actual tissue temperatures was obtained. The accuracy of temperature monitoring was better than 1{sup 0}C, while the spatial resolution was about 1 mm. These data suggest that the optoacoustic technique has the potential to be used for non-invasive, real-time temperature monitoring during thermotherapy.

  5. Real-Time IRI driven by GIRO data

    Science.gov (United States)

    Galkin, Ivan; Huang, Xueqin; Reinisch, Bodo; Bilitza, Dieter; Vesnin, Artem

    Real-time extensions of the empirical International Reference Ionosphere (IRI) model are based on assimilative techniques that preserve the IRI formalism which is optimized for the description of climatological ionospheric features. The Global Ionosphere Radio Observatory (GIRO) team has developed critical parts of an IRI Real Time Assimilative Model (IRTAM) for the global ionospheric plasma distribution using measured data available in real time from ~50 ionosondes of the GIRO network, The current assimilation results present global assimilative maps of foF2 and hmF2 that reproduce available data at the sensor sites and smoothly return to the climatological specifications when and where the data are missing, and are free from artificial sharp gradients and short-lived artifacts when viewed in time progression. Animated real-time maps of foF2 and hmF2 are published with a few minutes latency at http://giro.uml.edu/IRTAM/. Our real-time IRI modeling uses morphing, a technique that transforms the climatological ionospheric specifications to match the observations by iteratively computing corrections to the original coefficients of the diurnal/spatial expansions, used in IRI to map the key ionospheric characteristics, while keeping the IRI expansion basis formalism intact. Computation of the updated coefficient set for a given point in time includes analysis of the latest 24-hour history of observations, which allows the morphing technique to sense evolving ionospheric dynamics even with a sparse sensor network. A Non-linear Error Compensation Technique for Associative Restoration (NECTAR), one of the features in our morphing approach, has been in operation at the Lowell GIRO Data Center since 2013. The cornerstone of NECTAR is a recurrent neural network optimizer that is responsible for smoothing the transitions between the grid cells where observations are available. NECTAR has proved suitable for real-time operations that require the assimilation code to be

  6. Tokamak equilibrium reconstruction code LIUQE and its real time implementation

    Energy Technology Data Exchange (ETDEWEB)

    Moret, J.-M., E-mail: jean-marc.moret@epfl.ch; Duval, B.P.; Le, H.B.; Coda, S.; Felici, F.; Reimerdes, H.

    2015-02-15

    Highlights: • Algorithm vertical stabilisation using a linear parametrisation of the current density. • Experimentally derived model of the vacuum vessel to account for vessel currents. • Real-time contouring algorithm for flux surface averaged 1.5 D transport equations. • Full real time implementation coded in SIMULINK runs in less than 200 μs. • Applications: shape control, safety factor profile control, coupling with RAPTOR. - Abstract: Equilibrium reconstruction consists in identifying, from experimental measurements, a distribution of the plasma current density that satisfies the pressure balance constraint. The LIUQE code adopts a computationally efficient method to solve this problem, based on an iterative solution of the Poisson equation coupled with a linear parametrisation of the plasma current density. This algorithm is unstable against vertical gross motion of the plasma column for elongated shapes and its application to highly shaped plasmas on TCV requires a particular treatment of this instability. TCV's continuous vacuum vessel has a low resistance designed to enhance passive stabilisation of the vertical position. The eddy currents in the vacuum vessel have a sizeable influence on the equilibrium reconstruction and must be taken into account. A real time version of LIUQE has been implemented on TCV's distributed digital control system with a cycle time shorter than 200 μs for a full spatial grid of 28 by 65, using all 133 experimental measurements and including the flux surface average of quantities necessary for the real time solution of 1.5 D transport equations. This performance was achieved through a thoughtful choice of numerical methods and code optimisation techniques at every step of the algorithm, and was coded in MATLAB and SIMULINK for the off-line and real time version respectively.

  7. Efficacy of imidacloprid for control of cereal leaf beetle (Coleoptera: Chrysomelidae) in barley.

    Science.gov (United States)

    Tharp, C; Blodgett, S L; Johnson, G D

    2000-02-01

    The toxicity of imidacloprid to the cereal leaf beetle, Oulema melanopus (L.), was measured under laboratory and field conditions. Insect mortality and plant damage were determined from artificial and natural infestations of O. melanopus applied to various growth stages of barley. All rates of imidacloprid formulated and applied as a seed treatment caused >90% mortality to cereal leaf beetle larvae when barley was infested with eggs at the 4-leaf stage, but were ineffective when barley was infested with eggs at the early tillering or flag-leaf stages of barley. This window of susceptibility influenced results obtained in field trials where peak larval emergence did not occur until the early tillering stage of barley. The resulting mortality in plants from treated seeds never exceeded 40% in the field. Foliar imidacloprid, however, caused >90% mortality in the field, and may be another option in the management of the cereal leaf beetle.

  8. A distributed agent architecture for real-time knowledge-based systems: Real-time expert systems project, phase 1

    Science.gov (United States)

    Lee, S. Daniel

    1990-01-01

    We propose a distributed agent architecture (DAA) that can support a variety of paradigms based on both traditional real-time computing and artificial intelligence. DAA consists of distributed agents that are classified into two categories: reactive and cognitive. Reactive agents can be implemented directly in Ada to meet hard real-time requirements and be deployed on on-board embedded processors. A traditional real-time computing methodology under consideration is the rate monotonic theory that can guarantee schedulability based on analytical methods. AI techniques under consideration for reactive agents are approximate or anytime reasoning that can be implemented using Bayesian belief networks as in Guardian. Cognitive agents are traditional expert systems that can be implemented in ART-Ada to meet soft real-time requirements. During the initial design of cognitive agents, it is critical to consider the migration path that would allow initial deployment on ground-based workstations with eventual deployment on on-board processors. ART-Ada technology enables this migration while Lisp-based technologies make it difficult if not impossible. In addition to reactive and cognitive agents, a meta-level agent would be needed to coordinate multiple agents and to provide meta-level control.

  9. Barley (Hordeum vulgare L.) cysteine proteases: heterologous expression, purification and characterization

    DEFF Research Database (Denmark)

    Rosenkilde, Anne Lind; Dionisio, Giuseppe; Holm, Preben Bach

    2011-01-01

    During germination of barley seeds, mobilization of protein is essential and cysteine proteases accounts for more than 90 % of the total proteolytic activity in the degradation of barley seed storage proteins. Cysteine proteases exist as pro-enzyme and is activated through reduction of the active...... site cysteines and by removal of the pro-domain. The complement of cysteine proteases is comprehensive and for detailed studies of the individual components of this complement, a fast and efficient eukaryotic expression platform is highly desirable. A cDNA clone of the barley key cysteine endoprotease...

  10. Heterologous expression and purification of barley (Hordeum vulgare L.) cysteine protease in yeast

    DEFF Research Database (Denmark)

    Rosenkilde, Anne Lind; Dionisio, Giuseppe; Holm, Preben Bach

    2011-01-01

    The mobilization of protein during germination of barley seeds is essential and Cysteine Proteases accounts for more than 90 % of the total proteolytic activity in the degradation of barley seed storage proteins [1]. Cysteine proteases exist as pro-enzyme until activated through reduction...... of the active site cysteines and via removal of the pro-domain. The complement of cysteine proteases is comprehensive and for detailed studies of the individual components of this complement, a fast and efficient eukaryotic expression platform is highly desirable. The barley key cysteine protease, endoprotease...

  11. Real-time GNSS volcano deformation monitoring (Invited)

    Science.gov (United States)

    Lisowski, M.; Langbein, J. O.; Hudnut, K. W.

    2013-12-01

    We present comparisons of the precision obtained from several alternative real-time GNSS processing methods, and show how offsets caused by snow and ice on an antenna can be automatically identified in real time using signal-to-noise ratio (SNR) data. We monitor ground deformation using continuous GNSS stations installed on several volcanoes in the Cascade Range and elsewhere, and many of these stations transmit high-rate (1s) data in real-time. We examine real-time, high-rate station position solutions obtained with two implementations of centralized RTNet (GPS Solutions, Inc.) processing, and find that the precision is roughly the same for ambiguity-fixed network solutions and for ambiguity-fixed precise point position solutions (PPPAR). The PPPAR method uses satellite clock corrections provided by GPS Solutions from a network of Plate Boundary Observatory (PBO) stations in western Oregon. The precision of network solutions that include GPS and GLONASS data is similar to the GPS-only solutions, except at stations with a relatively poor view of the sky. An alternative method of processing the real-time GPS data uses clock corrections transmitted directly to the receiver, which then autonomously calculates and transmits positions. We will compare our RTNet results with autonomous point position solutions calculated using Trimble's CenterPoint RTX corrections. RTX performance in repeated, controlled, large antenna-motion tests by USGS and UNAVCO indicates that it meets requirements of USGS volcano-monitoring applications; more thorough testing and performance checks on an ongoing basis would be desirable. GNSS antennas on volcanoes often become temporarily coated with ice or buried by snow in the winter. In these situations, signal delays introduce an apparent offset in the monitoring station's position. We address this problem by implementing in real time a technique developed by Kristine Larson that uses changes in the signal-to-noise ratio (SNR) of GNSS signals

  12. The VLBA correlator: Real-time in the distributed era

    Science.gov (United States)

    Wells, D. C.

    1992-01-01

    The correlator is the signal processing engine of the Very Long Baseline Array (VLBA). Radio signals are recorded on special wideband (128 Mb/s) digital recorders at the 10 telescopes, with sampling times controlled by hydrogen maser clocks. The magnetic tapes are shipped to the Array Operations Center in Socorro, New Mexico, where they are played back simultaneously into the correlator. Real-time software and firmware controls the playback drives to achieve synchronization, compute models of the wavefront delay, control the numerous modules of the correlator, and record FITS files of the fringe visibilities at the back-end of the correlator. In addition to the more than 3000 custom VLSI chips which handle the massive data flow of the signal processing, the correlator contains a total of more than 100 programmable computers, 8-, 16- and 32-bit CPUs. Code is downloaded into front-end CPU's dependent on operating mode. Low-level code is assembly language, high-level code is C running under a RT OS. We use VxWorks on Motorola MVME147 CPU's. Code development is on a complex of SPARC workstations connected to the RT CPU's by Ethernet. The overall management of the correlation process is dependent on a database management system. We use Ingres running on a Sparcstation-2. We transfer logging information from the database of the VLBA Monitor and Control System to our database using Ingres/NET. Job scripts are computed and are transferred to the real-time computers using NFS, and correlation job execution logs and status flow back by the route. Operator status and control displays use windows on workstations, interfaced to the real-time processes by network protocols. The extensive network protocol support provided by VxWorks is invaluable. The VLBA Correlator's dependence on network protocols is an example of the radical transformation of the real-time world over the past five years. Real-time is becoming more like conventional computing. Paradoxically, 'conventional

  13. Determination of Uncalibrated Phase Delays for Real-Time PPP

    Science.gov (United States)

    Hinterberger, Fabian; Weber, Robert; Huber, Katrin; Lesjak, Roman

    2014-05-01

    Today PPP is a well-known technique of GNSS based positioning used for a wide range of post-processing applications. Using observations of a single GNSS receiver and applying precise orbit and clock information derived from global GNSS networks highly precise positions can be obtained. The atmospheric delays are usually mitigated by linear combination (ionosphere) and parameter estimation (troposphere). Within the last years also the demand for real-time PPP increased. In 2012, the IGS real-time working group started a pilot project to broadcast real-time precise orbits and clock correction streams. Nevertheless, real-time PPP is in its starting phase and currently only few applications make use of the technique although SSR-Messages are already implemented in RTCM3.1. The problems of still limited accuracy compared to Network-RTK as well as long convergence times might be solved by almost instantaneous integer ambiguity resolution at zero-difference level which is a major topic of current scientific investigations. Therefore a national consortium has carried out over the past 2 years the research project PPP-Serve (funded by the Austrian Research Promotion Agency - FFG), which aimed at the development of appropriate algorithms for real-time PPP with special emphasis on the ambiguity resolution of zero-difference observations. We have established a module which calculates based on GPS-reference station data-streams of a dense network (obtained from IGS via BKG) so-called wide-lane and narrow-lane satellite specific calibration phase delays. While the wide-lane phase delays are almost stable over longer periods, the estimation of narrow-lane phase delays has to be re-established every 24 hours. These phase-delays are submitted via a real-time module to the rover where they are used for point positioning via a PPP-model. This presentation deals with the process and obstacles of calculating the wide-lane and narrow-lane phase-delays (based on SD -observations between

  14. Two metallothionein gene family members in buckwheat: Expression analysis in flooding stress using Real Time RT-PCR technology

    Directory of Open Access Journals (Sweden)

    Majić Dragana B.

    2008-01-01

    Full Text Available Metallothioneins (MTs are an extensive and diverse family of small cysteine-rich proteins with metal-binding ability that are involved in metal homeostasis and detoxification. Two cDNA clones of the MT3 type, differing in 3’ UTRs, were isolated from the developing buckwheat seed cDNA library. Following sequence analyses, expression profiles during flooding stress were monitored by Real Time RT PCR technology.

  15. Cost evaluation of a DSN high level real-time language

    Science.gov (United States)

    Mckenzie, M.

    1977-01-01

    The hypothesis that the implementation of a DSN High Level Real Time Language will reduce real time software expenditures is explored. The High Level Real Time Language is found to be both affordable and cost-effective.

  16. Real-Time Walk Light Detection with a Mobile Phone.

    Science.gov (United States)

    Ivanchenko, Volodymyr; Coughlan, James; Shen, Huiying

    2010-07-01

    Crossing an urban traffic intersection is one of the most dangerous activities of a blind or visually impaired person's travel. Building on past work by the authors on the issue of proper alignment with the crosswalk, this paper addresses the complementary issue of knowing when it is time to cross. We describe a prototype portable system that alerts the user in real time once the Walk light is illuminated. The system runs as a software application on an off-the-shelf Nokia N95 mobile phone, using computer vision algorithms to analyze video acquired by the built-in camera to determine in real time if a Walk light is currently visible. Once a Walk light is detected, an audio tone is sounded to alert the user. Experiments with a blind volunteer subject at urban traffic intersections demonstrate proof of concept of the system, which successfully alerted the subject when the Walk light appeared.

  17. Real-time performance monitoring and management system

    Science.gov (United States)

    Budhraja, Vikram S.; Dyer, James D.; Martinez Morales, Carlos A.

    2007-06-19

    A real-time performance monitoring system for monitoring an electric power grid. The electric power grid has a plurality of grid portions, each grid portion corresponding to one of a plurality of control areas. The real-time performance monitoring system includes a monitor computer for monitoring at least one of reliability metrics, generation metrics, transmission metrics, suppliers metrics, grid infrastructure security metrics, and markets metrics for the electric power grid. The data for metrics being monitored by the monitor computer are stored in a data base, and a visualization of the metrics is displayed on at least one display computer having a monitor. The at least one display computer in one said control area enables an operator to monitor the grid portion corresponding to a different said control area.

  18. Real time markerless motion tracking using linked kinematic chains

    Science.gov (United States)

    Luck, Jason P.; Small, Daniel E.

    2007-08-14

    A markerless method is described for tracking the motion of subjects in a three dimensional environment using a model based on linked kinematic chains. The invention is suitable for tracking robotic, animal or human subjects in real-time using a single computer with inexpensive video equipment, and does not require the use of markers or specialized clothing. A simple model of rigid linked segments is constructed of the subject and tracked using three dimensional volumetric data collected by a multiple camera video imaging system. A physics based method is then used to compute forces to align the model with subsequent volumetric data sets in real-time. The method is able to handle occlusion of segments and accommodates joint limits, velocity constraints, and collision constraints and provides for error recovery. The method further provides for elimination of singularities in Jacobian based calculations, which has been problematic in alternative methods.

  19. Interactive Construction Digital Tools With Real Time Analysis

    DEFF Research Database (Denmark)

    Klitgaard, Jens; Kirkegaard, Poul Henning

    2007-01-01

    . An example of a prototype for a digital conceptual design tool with integrated real time structural analysis is presented and compared with a more common Building Information Modelling (BIM) approach. It is concluded that a digital conceptual design tool with embedded real time structural analysis could......The recent developments in computational design tools have evolved into a sometimes purely digital process which opens up for new perspectives and problems in the sketching process. One of the interesting possibilities lay within the hybrid practitioner- or architect-engineer approach, where...... an architect-engineer or hybrid practitioner works simultaneously with both aesthetic and technical design requirements. In this paper the problem of a vague or not existing link between digital design tools, used by architects and designers, and the analysis tools developed by and for engineers is considered...

  20. REAL TIME REGISTRATION OF THE ELECTROPHYSIOLOGICAL SIGNALS SPECTRA

    Directory of Open Access Journals (Sweden)

    D. V. Tolkovich

    2014-01-01

    Full Text Available The paper deals with the issues of spectra acquisition for the electrophysiological signals, diagnostics result dependence on the noise level and expert’s qualification. A real-time module for amplitude-frequency spectra estimation of electrophysiological signals is designed. A general diagram of connection between measuring module and spectrum analyzer (as a part of multifunctional diagnostic system is shown. The module is realized on the base of Cubieboard A20 minicomputer. It provides continuous decomposition of signals (up to 32 channels into fragments of equal length and their independent mathematical processing for each measuring channel. The suggested device makes it possible to analyze the real-time dynamics of spectrum changes.