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Sample records for banano musa aaa

  1. EFECTO DE DOS TIPOS DE FUNDAS SOBRE EL FRUTO DE BANANO (Musa AAA

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    Alfonso Vargas-Calvo

    2011-01-01

    Full Text Available El objetivo de este estudio fue determinar el efecto de dos fundas en la protección del racimo de banano (Musa AAA . En dos épocas climáticas (adversa y favorable bajo condiciones del Ca ribe de Costa Ri ca se evaluaron dos fundas: 1- azul Sa nta Lucía (bifentrina 0,1%, polie tileno de 12,7 ¿ de grosor, con perforaciones de 4 mm y 86,4 cm de ancho y 2- transparente con aditivos para filtrar la luz ultravioleta e infrarroja (bifentrina 0,1%, 20,3 ¿ de grosor, con perforaciones de 4 mm y 88,9 cm de ancho. El peso del racimo así como el grosor y la longitud del fruto central de la fila externa en la segunda, cuarta y sexta mano no difirieron (P> 0,0556 entre ambas fundas. Tampoco hubo diferencias entre estas en la apa rie ncia del racimo (P>0,4699 ni en la firmeza de la cáscara en grado 1 de maduración (P= 0,6268. En las varia bles de medición del color del fruto solamente L* presentó un valor (56,48 más alto (P=0,0109 con la funda transparente fotosensible, mie ntras que las otras dos varia bles relacionadas (a* y b* no fueron diferentes (P>0,1011 entre las fundas. El incremento adicional de 7,6 ¿ en el grosor del polie tileno de la funda transparente con respecto a la azul Sa nta Lucía, no ocasionó un incremento productivo ni una mejor apa rie ncia del racimo de banano y sus frutos.

  2. Caracterización de harina y almidón de frutos de banano gros michel (musa acuminata aaa)

    OpenAIRE

    Jairo Montoya-López; Víctor Dumar Quintero-Castaño; Juan Carlos Lucas-Aguirre

    2014-01-01

    En el estudio se determinaron las características fisicoquímicas, térmicas y reológicas de la harina y el almidón de frutos de banano Gros Michel (Musa acuminata) cosechado en fincas del departamento del Quindío, Colombia. En el análisis proximal, la harina presentó un contenido de fibra de 18.82% y el almidón presentó contenidos de proteína de 1.92%, grasa de 5.3% y fibra de 2.76%. La harina presentó la temperatura más alta de absorción de calor (68.88 °C) y su entalpia de gelatinización f...

  3. Utilización del método de superficie de respuesta para formular una base de banano (Musa AAA para batidos

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    Marta Gamboa White

    2010-06-01

    Full Text Available El Método de Superficie de Respuesta se utiliza para optimizar o reformular productos. Se usa principalmente para economizar dinero y reducir el tiempo de pruebas, al disminuir el número de ensayos. Se presenta la aplicación del Método de Superficie de Respuesta para desarrollar una base a partir de bananos (Musa AAA var. Cavendish cv. Gran Enano de rechazo de exportación a manera de caso para estudiar su uso. Por medio de una encuesta se identificó que el dulzor, espumosidad y sabor fueron los atributos que los consumidores consideraron más importantes en un batido. Con base en estos atributos y con los ingredientes: pulpa de banano, goma guar y azúcar, se elaboraron y seleccionaron varias mezclas base, que fueron degustadas, en forma de batidos en leche, por un grupo de 90 consumidores. Por medio del diseño de superficie de respuesta se determinó que el batido con la mayor aceptación estuvo constituido por un 77,0 % de leche y un 23,0 % de mezcla base, compuesta esta última por 0,12 % de goma guar, 80,50 % de pulpa de banano y un 19,50 % de azúcar. Un Análisis de Componentes Principales permitió determinar que el contenido de sacarosa, que define el grado de dulzor, fue el atributo más importante para la aceptación del batido y se demostró que la combinación del Método de Superficie de Respuesta con el Análisis de Componentes Principales constituyó una herramienta útil en la formulación y optimización de productos, sobre todo para entender la interrelación de las variables.

  4. Caracterización de harina y almidón de frutos de banano Gros Michel (Musa acuminata AAA

    Directory of Open Access Journals (Sweden)

    Jairo Montoya-López

    2015-01-01

    Full Text Available En el estudio se determinaron las características fisicoquímicas, térmicas y reológicas de la harina y el almidón de frutos de banano Gros Michel (Musa acuminata cosechado en fincas del departamento del Quindío, Colombia. En el análisis proximal, la harina presentó un contenido de fibra de 18.82% y el almidón presentó contenidos de proteína de 1.92%, grasa de 5.3% y fibra de 2.76%. La harina presentó la temperatura más alta de absorción de calor (68.88 °C y su entalpía de gelatinización fue de 2.17 J/g; mientras que para el almidón estos valores fueron de 48.36 °C y 44.62 J/g, respectivamente. El análisis termogravimétrico (TGA de la harina o temperaturas en las cuales se registra la descomposición de carbohidratos (componentes de bajo peso molecular y polisacáridos (componentes de alto peso molecular fueron, respectivamente, de 284.51 °C y 470.42 °C; y para el almidón fueron de 307.51 °C y 500.46 °C. Los gránulos de almidón de banano tienen forma elipsoidal con un tamaño longitudinal promedio de 39.39 µm y tamaño transversal promedio de 29.47 µm. Los difractogramas de rayos X mostraron patrones de difracción tipo B. Los viscoamilogramas mostraron que para la harina la temperatura de inicio de gelatinización (Tg es de 76.3 °C, la viscosidad máxima de 1120 cP, y para el almidón la Tg fue de 70.75 °C y la viscosidad máxima de 2087 cP.

  5. Multiplicación de hongos micorriza arbuscular (h.m.a) y efecto de la micorrización en plantas micropropagadas de banano (musa aaa cv. gran enano) (musaceae).

    OpenAIRE

    Usuga Osorio, Carmen Elena; Castañeda Sánchez, Darío Antonio; Franco Molano, Ana Esperanza

    2011-01-01

    Se evaluó el proceso de multiplicación de hongos que forman micorriza arbuscular (HMA), para lo cual se usaron diferentes tipos de inóculos entre ellos nativos de agroecosistemas bananeros del Urabá (Antioquia-Colombia), en sustrato sólido, con diferentes plantas hospedadoras y la infectividad y efectividad sobre plantas de banano (Musa AAA cv. Gran Enano). La colonización micorrizal promedio general de los HMA a las plantas trampa fue de 37,76 ± 21,86 %, con respecto a este porcentaje, las p...

  6. MULTIPLICACIÓN DE HONGOS MICORRIZA ARBUSCULAR (H.M.A) Y EFECTO DE LA MICORRIZACIÓN EN PLANTAS MICROPROPAGADAS DE BANANO (Musa AAA cv. Gran Enano) (Musaceae) MULTIPLICATION OF ARBUSCULAR MYCORRHIZAE FUNGI (AMF) AND MYCORRHIZATION EFFECT IN MICROPROPAGATED PLANTS OF BANANA (Musa AAA cv. ‘Gran Enano’) (Musaceae)

    OpenAIRE

    Carmen Elena Usuga Osorio; Darío Antonio Castañeda Sánchez; Ana Esperanza Franco Molano

    2008-01-01

    Se evaluó el proceso de multiplicación de hongos que forman micorriza arbuscular (HMA), para lo cual se usaron diferentes tipos de inóculos entre ellos nativos de agroecosistemas bananeros del Urabá (Antioquia-Colombia), en sustrato sólido, con diferentes plantas hospedadoras y la infectividad y efectividad sobre plantas de banano (Musa AAA cv. Gran Enano). La colonización micorrizal promedio general de los HMA a las plantas trampa fue de 37,76 ± 21,86 %, con respecto a este porcentaje, las p...

  7. EFECTO DE LA MICORRIZACIÓN Y LA FERTILIZACIÓN EN LA ACUMULACIÓN DE BIOMASA EN PLANTAS DE BANANO (Musa AAA cv. Gran Enano) (Musaceae) MICORRHIZATION AND FERTILIZATION EFFECT ON BIOMASS ACCUMULATION IN BANANA PLANTS (Musa AAA cv. Gran Enano) (Musaceae)

    OpenAIRE

    Carmen Elena Usuga Osorio; Darío Antonio Castañeda Sánchez; Ana Esperanza Franco Molano; Felipe Andrés Gómez Velásquez; Carlos Adrián Lopera Agudelo

    2008-01-01

    Bajo condiciones de invernadero (ubicado en el municipio de Bello - Antioquia (Colombia) se evaluó el efecto independiente y combinado de los factores: tipo de inóculo de Hongos Micorriza Arbuscular (HMA), fertilización y aplicación de materia orgánica sobre el porcentaje de asociación de HMA en plantas de banano (Musa AAA cv. Gran Enano), así como en la acumulación de materia seca foliar y radical. Dentro del factor tipo de inóculo, se evaluaron inóculos nativos, de agroecosistemas bananeros...

  8. EFECTO DEL ALMIDÓN RESISTENTE DE BANANO (Musa cavendish AAA) SOBRE EL CONTROL METABÓLICO EN RATAS WISTAR CON DIETA ALTA EN SACAROSA

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    V Olvera-Hernández; MA Aparicio-Trápala; JL Ble-Castillo; JM Muñoz-Cano; L Rodríguez-Blanco

    2012-01-01

    En este estudio se determinaron los efectos del almidón resistente de banano gran enano (Musa cavendish) sobre los cambios metabólicos en sangre, en ratas con dieta alta en sacarosa. Se utilizaron 32 ratas Wistar macho recién destetadas de 150 g, suministradas con sacarosa al 30% (P/V) ad libitum durante 29 semanas. Posteriormente se administraron las siguientes dietas: dieta normal (DN) (control), almidón de yuca (AY); almidón resistente (AR) y fórmula de almidón resistente con leche de vaca...

  9. Descripción morfoagronómica de materiales de plátano (Musa AAB, ABB) y banano (Musa AAA) cultivados en San Andrés Isla Morpho-agronomic Description of Plantain (Musa AAB, ABB) and Banana (Musa AAA) Materials Grown in San Andres Island

    OpenAIRE

    Oscar Javier Parra Pachón; Daniel Gerardo Cayón Salinas; Jaime Polanía Vorenber

    2009-01-01

    Durante el primer semestre de 2005 se estudiaron los cultivares de plátano y banano en fincas y parcelas de 15 agricultores típicos participantes en programas conjuntos de la Secretaría de Agricultura y Pesca de San Andrés Isla y de la Universidad Nacional de Colombia sede Caribe. A partir de descriptores de INIBAP, IPGRI y CIRAD y revisiones bibliográficas.se describen morfológicamente los materiales de Musa cultivados en la Isla, así como las prácticas de los productores isleños, Se identif...

  10. EFECTO DE LA MICORRIZACIÓN Y LA FERTILIZACIÓN EN LA ACUMULACIÓN DE BIOMASA EN PLANTAS DE BANANO (Musa AAA cv. Gran Enano (Musaceae MICORRHIZATION AND FERTILIZATION EFFECT ON BIOMASS ACCUMULATION IN BANANA PLANTS (Musa AAA cv. Gran Enano (Musaceae

    Directory of Open Access Journals (Sweden)

    Carmen Elena Usuga Osorio

    2008-06-01

    Full Text Available Bajo condiciones de invernadero (ubicado en el municipio de Bello - Antioquia (Colombia se evaluó el efecto independiente y combinado de los factores: tipo de inóculo de Hongos Micorriza Arbuscular (HMA, fertilización y aplicación de materia orgánica sobre el porcentaje de asociación de HMA en plantas de banano (Musa AAA cv. Gran Enano, así como en la acumulación de materia seca foliar y radical. Dentro del factor tipo de inóculo, se evaluaron inóculos nativos, de agroecosistemas bananeros y ecosistemas naturales del Urabá (Antioquia-Colombia, uno comercial y la especie Acaulospora morrowiae; con respecto a la fertilización se probó la mitad, completa y dos veces la dosis de la fertilización recomendada de acuerdo al análisis de suelo y a los requerimientos de la planta, y cada uno de estos factores con y sin la aplicación de materia orgánica; como testigos se usaron, la no aplicación del respectivo factor. Se usó como material vegetal plantas de banano micropropagadas del grupo Cavendish cv. Gran Enano (AAA. El sustrato utilizado para el crecimiento de las plantas de banano se compuso de suelo y arena en relación 70/30 v/v. El suelo se obtuvo de la granja experimental de Augura, ubicado en el municipio de Carepa en la región de Urabá. Los resultados encontrados, muestran que los factores que más incidieron en la asociación así como en la acumulación de biomasa en toda la planta son la micorrización y la adición de materia orgánica. Los resultados, también muestran un comportamiento positivo respecto al uso de inóculos nativos de agroecosistemas bananeros, con bajas aplicaciones de fertilizantes.The effects of independent an combined factors such as inoculum type, fertilization and organic matter application on the percentage of association of ‘H.M.A’ in banana plants (Musa AAA cv. ‘Gran Enano’, and on the accumulation of leaves and rrots material, were evaluated under greenhouse conditions. Natives samples

  11. Nuevo método para la selección rapida de plantas de banano (Musa spp. AAA cv. Grande naine transformadas con gen bar procedentes de campo, empleando glufosinato de amonio

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    Rafael Gómez-Kosky

    2010-10-01

    Full Text Available Título en inglés: New method for rapid selection of banana (Musa spp. AAA cv. Grande naine plants with bar gene in the field using ammonium gluphosinate Resumen Los genes marcadores de la selección son ampliamente utilizados para la transformación eficiente de diferentes cultivos, sin embargo, existen muy pocas referencias sobre el desarrollo de métodos de selección temprana en campo de plantas supuestamente transformadas con marcador de selección tipo herbicidas. La presente investigación tuvo como objetivo evaluar el efecto del glufosinato de amonio a partir del herbicida Finale en los fragmentos de hojas de plantas de campo cultivados in vitro, para la selección de líneas transformadas de banano cv. Grande naine (Musa spp. AAA, con una construcción que porta como marcador de selección el gen bar. A partir del método desarrollado con el empleo de fragmentos de hojas de plantas procedentes de campo, cultivados in vitro en el medio de cultivo agar al 1% más agua y 30,0 g/l-1 de glufosinato de amonio se logró diferenciar las dos líneas transformadas del control no transformado, a partir de la expresión del gen bar. Ambas líneas fueron positivas en el análisis molecular de PCR. Estos resultados permiten disponer de una herramienta útil y simple como parte de un protocolo de transformación genética para el cultivo del banano, siendo el primer informe de este resultado a nivel internacional. Palabras clave: Musa; transformación genética; gen bar; glufosinato de amonio. Abstract Selector marker genes are widely used for the efficient transformation of different crops; however, there are very few references on the development of early selection methods in the fields of potentially transformed plants for herbicides. The objective of this research was to evaluate the effect of ammonium gluphosinate from the herbicide Finale in leaf fragments from in vitro plants grown in the fields for the selection of putative transformed

  12. DETECCIÓN DE BACTERIAS BENÉFICAS EN SUELO CON BANANO (Musa AAA Simmonds) cultivar 'Gran enano' Y SU POTENCIAL PARA INTEGRAR UN BIOFERTILIZANTE

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    Y Córdova-Bautista; MC Rivera-Cruz; R Ferrera-Cerrato; JJ Obrador-Olán; V Córdova-Ávalos

    2009-01-01

    Se determinaron las densidades de bacterias totales fijadoras de N de vida libre en suelo hasta 110 cm de profundidad y en suelo rizosférico de la planta de banano establecido en un Fluvisol éutrico. Se colectaron muestras de suelo en los seis horizontes del perfil y en 30 puntos de muestreo rizosférico (0-10 cm de profundidad) distribuidos en forma aleatoria en 10 000 m2. Las poblaciones de Azospirillum y Azotobacter se aislaron hasta profundidades de 59 y 110 cm, respectivamente. Los gremio...

  13. MULTIPLICACIÓN DE HONGOS MICORRIZA ARBUSCULAR (H.M.A Y EFECTO DE LA MICORRIZACIÓN EN PLANTAS MICROPROPAGADAS DE BANANO (Musa AAA cv. Gran Enano (Musaceae MULTIPLICATION OF ARBUSCULAR MYCORRHIZAE FUNGI (AMF AND MYCORRHIZATION EFFECT IN MICROPROPAGATED PLANTS OF BANANA (Musa AAA cv. ‘Gran Enano’ (Musaceae

    Directory of Open Access Journals (Sweden)

    Carmen Elena Usuga Osorio

    2008-06-01

    Full Text Available Se evaluó el proceso de multiplicación de hongos que forman micorriza arbuscular (HMA, para lo cual se usaron diferentes tipos de inóculos entre ellos nativos de agroecosistemas bananeros del Urabá (Antioquia-Colombia, en sustrato sólido, con diferentes plantas hospedadoras y la infectividad y efectividad sobre plantas de banano (Musa AAA cv. Gran Enano. La colonización micorrizal promedio general de los HMA a las plantas trampa fue de 37,76 ± 21,86 %, con respecto a este porcentaje, las plantas B (Brachiaria decumbens y S (Sorgum vulgare fueron las que más favorecieron la simbiosis. Teniendo en cuenta el sustrato, el S2 (Arena 50 - suelo 50 y el S6 (Vermiculita 50-suelo 50 permitieron expresiones significativamente mayores respecto a los demás. El Sorgum vulgare y Pueraria phaseoloides y en el sustrato S1 (Arena 30 - suelo 70, se encontró un mayor número de esporas. La combinación planta-sustrato que más favoreció la asociación fue la planta trampa B en los sustratos S2 y S4 (cascarilla de arroz 50-suelo50 y la producción de esporas fueron las plantas K y S en el sustrato S1. La asociación micorrícica general en plantas de banano provenientes de cultivo de tejidos fue de 48,74 ± 30,44. No se encontraron diferencias significativas (P > 0,05 entre plantas de cero días con plantas de 30 de aclimatadas. Los inóculos que significativamente favorecieron la asociación fueron los provenientes de agroecosistemas bananeros al compararse con el inóculo comercial y el proveniente de ecosistemas naturales del Urabá. El mayor peso seco foliar y radical se encontró en plántulas de banano inoculadas con I5 (Inóculo proveniente de agroecosistema bananeros de la zona de estudio. Para las variables de crecimiento no se encontraron diferencias.The process of multiplication of arbuscular mycorrhizae fungi (AMF from indigenous banana agro-environments from Urabá (Antioquia - Colombia was evaluated, using solid substrate, with different

  14. Nuevo método para la selección rapida de plantas de banano (Musa spp. AAA cv. Grande naine) transformadas con gen bar procedentes de campo, empleando glufosinato de amonio

    OpenAIRE

    Rafael Gómez-Kosky; Maritza Reyes Vega; Idalmis Bermúdez Caraballoso; Borys Chong Pérez; Yelenys Alvarado Capo

    2010-01-01

    Título en inglés: New method for rapid selection of banana (Musa spp. AAA cv. Grande naine) plants with bar gene in the field using ammonium gluphosinate Resumen Los genes marcadores de la selección son ampliamente utilizados para la transformación eficiente de diferentes cultivos, sin embargo, existen muy pocas referencias sobre el desarrollo de métodos de selección temprana en campo de plantas supuestamente transformadas con marcador de selección tipo herbicidas. La presente invest...

  15. EFFECT OF NUMBER OF FUNCTIONAL LEAVES AT FLOWERING ON YIELD OF BANANA GRAND NAINE (Musa AAA Simmonds EFECTO DEL NÚMERO DE HOJAS FUNCIONALES A LA FLORACIÓN SOBRE LA PRODUCCIÓN DE BANANO GRAN ENANO (Musa AAA Simmonds

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    Carolina Rodríguez González

    2012-06-01

    Full Text Available Abstract. The effect of the selective removal of leaves at flowering on yield, growth, maturity and quality of Grand Nain banana fruits was evaluated. The present study was conducted in three regions of Urabá (Colombia with precipitation low (experiment 1, medium (experiment 2 and high (experiment 3. The experimental design used randomized complete blocks, four treatments (6, 8, 10 and 12 leaves from the start of flowering and three replications. Every 15 days, the thickness (cm and length (cm of central fruit from the second hand were measured and two fruits were taken to record fresh weight and dry weight. At harvest, leaf number, bunch weight (kg, and the thickness (cm and length (cm of central fruit from the second hand were measured. During fruit ripening (8 and 15 days, the contents of starch and sugars in the pulp were determined. The thickness and length of the fruit, bunch weight and the box-stem ratio were not affected (PResumen. Se evaluó el efecto de la eliminación selectiva de hojas a la floración sobre la producción, crecimiento, maduración y calidad de los frutos de banano Gran Enano. El estudio se realizó en tres zonas de Urabá (Colombia, con precipitación baja (experimento 1, media (experimento 2 y alta (experimento 3. Se usó un diseño de bloques completamente al azar, cuatro tratamientos (6, 8, 10 y 12 hojas desde el inicio de floración y tres repeticiones. Cada 15 días se midieron el grosor (cm y la longitud (cm del fruto central de la segunda mano y se tomaron dos frutos más para registrar el peso fresco y el peso seco. A la cosecha se midieron el número de hojas, el peso del racimo (kg, el grosor (cm y la longitud (cm del fruto central de la segunda mano. Durante la maduración de los frutos (8 y 15 días se determinaron los contenidos de almidón y azúcares en la pulpa. El grosor y la longitud del fruto, peso del racimo y la cantidad de cajas a partir de un racimo (ratio, no fueron afectados (P<0,05 por la

  16. Fisiología de maduración postcosecha de banano Variedad Nanica (Musa Cavendishii

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    Fanny Villamizar de Borrero

    2011-02-01

    Full Text Available Después de la cosecha, gran número de frutas llamadas climatéricas sufren profundas transformaciones bioquímicas que influyen directamente la calidad de esos productos. La respiración medida como la cantidad de CO2 producido por Kg de producto por hora, es el proceso metabólico más importante entre los demás que ocurren en las frutas después de la cosecha, siendo que su intensidad se refleja en la mayor o menor deterioración del producto, para una determinada condición de almacenamiento. La importancia del conocimiento de la intensidad respiratoria de un producto, hace posible relacionar algunas de las transformaciones físicas y bioquímicas tales como la variación del peso, volumen, firmeza, desarrollo de azúcares, ácidos, etc., que en conjunto son responsables por la buena calidad y conservación posterior. En el presente trabajo se tuvo como objetivo principal el estudio de la tasa respiratoria del banano, variedad NANICA (MUSA Cavendishii, en dos condiciones diferentes de almacenamiento: 26" C y 60% de humedad relativa y 15" C Y 90% de humedad relativa. Se estudió paralelamente su incidencia en los parámetros físicos, de peso, peso especifico, volumen, firmeza y químicos en sólidos solubles, pH, acidez, azúcares reductores y azúcares totales. Los resultados obtenidos pueden considerarse de gran utilidad para el estudio de los parámetros que intervienen en procesos de almacenamiento, empaque, transporte y conservación de este producto.

  17. Radiation dose assessment of musa acuminata - triploid (AAA)

    International Nuclear Information System (INIS)

    Bananas are radioactive due to the presence of the radioisotope-40K. This imposes a possible health risk to the general public. This study intended to assess the annual equivalent dosages and the annual effective dosage committed by the body. This seeks to benefit the general public, students and researchers, and entrepreneurs. Using atomic absorption spectrophotometry, lakatan banana (Musa acuminata-triploid (AAA), the most purchased variety cultivated in Barangay Adlawon, Cebu City, Philippines, was found to contain 0.53 g of total potassium for every 100 g of its fresh fruit wherein 6.2 x 10-5 g of which is potassium-40. Based on its 40K content banana was calculated to have a radioactivity of 16 Bq/100 g. it was found out that the body is exposed to radiation dosages ranging from 2.8 x 10-3 rem annually by eating 100 g of lakatan bananas everyday. Conversely, it is equivalent to the annual effective dosage of 0.0043 rem; the amount at which the body of an individual is uniformly exposed. However, no or extremely minute health risk was determined by just eating bananas. In fact, to exceed the radiation dose limits set by the International Commission on Radiation Protection, an individual may eat 116 kg of lakatan bananas everyday for a year. Fertilizers may be the major source of the radioisotope - 40K and assimilated by the plants. (author)

  18. Caracterización morfológica y contenido de almidón resistente y disponible en bananos (Musa sapientum) exportables del Ecuador

    OpenAIRE

    Edison Omar Martínez-Mora

    2015-01-01

    Introducción: La investigación tuvo como objetivo fundamental, la caracterización morfológica del almidón nativo de banano (Musa sapientum), contenido en almidón resistente (AR) y disponible (AD), de las variedades de banano de exportación: Cavendish (AC), Filipino (AF), Orito (AO) y Valery (AV).Material y Métodos: Se determinó tamaño y forma del gránulo de almidón a través de microscopia electrónica de barrido. La cuantificación del AR por medición de su fracción no hidrolizada, luego de un ...

  19. Análisis de patrones morfológicos y anatómicos en la embriogénesis somática del banano Williams (AAA)

    OpenAIRE

    Maribel Ramírez-Villalobos; Eva García; Helga Lindorf

    2012-01-01

    Título en ingles: Analysis of the morphological and anatomical patterns in the somatic embryogenesis of Williams banana (AAA)Resumen: La embriogénesis somática representa una herramienta esencial en el mejoramiento genético y en la micropropagación clonal masiva de bananos mejorados. En el presente trabajo se analizaron los patrones morfológicos y anatómicos que ocurren durante la embriogénesis somática del banano Williams, dirigidos a conocer y mejorar este proceso. En la investigación se e...

  20. Characterisation of pectins extracted from banana peels (Musa AAA) under different conditions using an experimental design.

    Science.gov (United States)

    Happi Emaga, Thomas; Ronkart, Sébastien N; Robert, Christelle; Wathelet, Bernard; Paquot, Michel

    2008-05-15

    An experimental design was used to study the influence of pH (1.5 and 2.0), temperature (80 and 90°C) and time (1 and 4h) on extraction of pectin from banana peels (Musa AAA). Yield of extracted pectins, their composition (neutral sugars, galacturonic acid, and degree of esterification) and some macromolecular characteristics (average molecular weight, intrinsic viscosity) were determined. It was found that extraction pH was the most important parameter influencing yield and pectin chemical composition. Lower pH values negatively affected the galacturonic acid content of pectin, but increased the pectin yield. The values of degree of methylation decreased significantly with increasing temperature and time of extraction. The average molecular weight ranged widely from 87 to 248kDa and was mainly influenced by pH and extraction time. PMID:26059123

  1. Caracterização do grânulo de amido de bananas (Musa AAA-Nanicão e Musa AAB-Terra Characterization of starch granules from bananas Musa AAA-Nanicão and Musa AAB-Terra

    Directory of Open Access Journals (Sweden)

    M.C.J. Freitas

    2005-06-01

    Full Text Available O amido de bananas tem sido pesquisado na área de nutrição a partir da introdução do conceito de Amido Resistente. O amido de Musa AAA-Nanicão e Musa AAB-Terra foram caracterizados quanto as suas respostas fisiológicas [12]. Em continuidade, o presente trabalho estudou características físicas e morfológicas dos grânulos de amido de ambas as espécies de banana comparando-as com amido nativo de milho comercial. O amido de bananas foi extraído segundo CHIANG, CHU & CHU [3]. A morfologia dos grânulos foi realizada após tratamento enzimático in vitro a 37°C/24h com alfa-amilase pancreática. Foram efetuados os respectivos amilogramas e difractogramas de raios-X. Os grânulos de amido da Musa Tipo AAA-Nanicão apresentaram comprimento entre 30-40µm. Em Musa AAB-Terra, os grânulos, também ovais e alongados, eram um pouco menores, 20-30µm. A corrosão enzimática in vitro iniciava-se sobre a superfície anteriormente lisa e formavam estrias superficiais e apicais. A Microscopia Eletrônica de Varredura (MEV mostrou que a hidrólise in vitro por 24 horas foi pequena e ocorria sobretudo nas camadas amorfas dos grânulos de ambas as espécies. O padrão de corrosão demonstrou-se distinto daquele ocorrido no amido de milho. As suspensões de amido de bananas ao viscosímetro demonstraram forte capacidade de hidratação e menor capacidade de retrogradação em relação ao milho, sobretudo do amido de Musa Tipo AAA-Nanicão; o amido de Musa Tipo AAB-Terra apresentou maior estabilidade de pasta. Na análise de difração de raios-X, os grânulos de bananas apresentaram padrão tipo B e C para Musa Tipo AAA-Nanicão e Musa Tipo AAB-Terra, respectivamente. Conclui-se que os amidos de Musa AAA-Nanicão e Musa AAB-Terra são estruturalmente distintos, justificando as respostas fisiológicas distintas encontradas posteriormente pelos mesmos autores. As distinções das propriedades físicas e bioquímicas obtidas para os grânulos, embora

  2. Formulation and evaluation of semisolid jelly produced by Musa acuminata Colla (AAA Group) peels

    Institute of Scientific and Technical Information of China (English)

    Noor Azwani Mohd Rasidek; Mariam Firdhaus Mad Nordin; Kamyar Shameli

    2016-01-01

    Objective: To study the jelly formulation produced by Musa acuminata Colla (AAA Group) peels and evaluate its antioxidant properties which are related to the product quality. Methods: The formulations of peel jelly were established under two-level full factorial designs within two blocks and one center point. Regarding response optimizer, the amount of sugar and citric acid was obtained; hence, the peel jellies were produced. The evaluation of antioxidant properties was conducted by using total phenolic content (TPC) assay and 1,1 diphenyl-2-picrylhydrazyl (DPPH) free radical assay. Results: The TPC of peel powder varied from 91.8 to 602.26 mg gallic acid equivalents/100 g dry weight, and 5%–7% peel jellies had phenolic content ranging from 29.38 to 48.31 mg gallic acid equivalents/100 g dry weight. The results of DPPH test indicated that at 10 mg/mL, the peel powder showed 89% DPPH inhibition, while 7% peel jelly prominently exhibited 84% DPPH inhibition. The correlation between DPPH IC50 value and TPC of peel powder as well as peel jelly was quite reasonably high with correlation coefficient ranging from 0.843 7 to 0.995. Conclusions: TPC can be used as an indicator in assessing the antioxidant activity of fruits and vegetables. The present investigation reveals that TPC is mainly responsible for DPPH free radical scavenging capacity.

  3. Morpho-agronomic description of plantain (musa aab, abb) and banana (musa aaa) materials grown in san andres island

    OpenAIRE

    Parra Pachón, Oscar Javier; Cayón Salinas, Daniel Gerardo; Polanía Vorenberg, Jaime

    2010-01-01

    During the first semester, 2005 we studied the plantain and banana cultivars in small farms of 15 volunteer regular producers, who were participating in joint programs of the Agriculture and Fisheries Secretariat of San Andres Island and the Colombian National University Caribbean Headquarters. We described morphologically the Musa cultivars identified in the island, as well as agricultural practices of the island producers, using the INIBAP, IPGRI and CIRAD (1996) descriptors and bibliograph...

  4. Antibacterial and Antioxidant Activities of Banana (Musa, AAA cv. Cavendish Fruits Peel

    Directory of Open Access Journals (Sweden)

    Matook S. Mokbe

    2005-01-01

    Full Text Available The fresh green and yellow banana peel of, (Musa, cv. Cavendish fruits were treated with 70% acetone, which were partitioned with chloroform (CHCl3 and ethyl acetate (EtOAc, sequentially. The antioxidant activities of the extracts were evaluated by using the thiocyanate method, ß-carotene bleaching method and 1,1-diphenyl-2-picrylhydrazyl (DPPH free radical elimination. While, antimicrobial activities of the extracts and isolated components were evaluated using paper disc methods and Minimum Inhibition Concentration (MIC. The EtOAc and water soluble fractions of green peel displayed high antimicrobial and antioxidant activity, respectively. Antioxidant activity of water extracts was comparable to those of synthetic antioxidants such as butylated hydroxyanisole and butylated hydroxytoluene. Among all isolated components ß-sitosterol, malic acid, succinic acid, palmatic acid, 12-hydroxystrearic acid, glycoside, the d-malic and 12-hydroxystrearic acid were the most active against all the Gram-negative and positive bacterial species tested. The MIC of d-malic and succinic acid was varying between 140-750 ppm, respectively.

  5. Altered Response to Biotic and Abiotic Stress in Tissue Culture-Induced Off-Type Plants of East African Highland Banana (Musa AAA East Africa)

    OpenAIRE

    Theodosy Msogoya; Brian Grout

    2008-01-01

    This study was conducted to investigate black sigatoka disease and drought stress responses of off-type plants derived from shoot-tip micropropagation of East African highland banana (Musa AAA East Africa) landrace Uganda. Results showed that the off-type plants were more (p<0.05) tolerant to black sigatoka disease with the infection index of 17.5% compared to 30.1 and 22.8% of the micropropagation (MP) derived phenotypically normal plants and Conventional Propagation (CP) derived p...

  6. Karyotypic and 2C Nuclear DNA Size Instability in vitro Induced Off-Types of East African Highland Banana (Musa AAA East Africa)

    OpenAIRE

    Theodosy Msogoya; Brian Grout; Andy Roberts

    2008-01-01

    This study was conducted to determine chromosome number and 2C nuclear DNA content in tissue culture induced off-type banana (Musa AAA East Africa) landrace Uganda with tolerance black sigatoka disease, susceptibility to water stress, sparsely black-blotched pseudostems, taller pseudostems, late fruit maturation, altered inflorescence and higher fruit dry matter content. The off-type banana appeared to have higher (p<0.05) frequency of 31 and 32 chromosomes at 15.1 and 13.6%, respect...

  7. Análisis de patrones morfológicos y anatómicos en la embriogénesis somática del banano Williams (AAA

    Directory of Open Access Journals (Sweden)

    Maribel Ramírez-Villalobos

    2012-02-01

    Full Text Available Normal 0 21 false false false ES-CO X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} Título en ingles: Analysis of the morphological and anatomical patterns in the somatic embryogenesis of Williams banana (AAAResumen: La embriogénesis somática representa una herramienta esencial en el mejoramiento genético y en la micropropagación clonal masiva de bananos mejorados. En el presente trabajo se analizaron los patrones morfológicos y anatómicos que ocurren durante la embriogénesis somática del banano Williams, dirigidos a conocer y mejorar este proceso. En la investigación se establecieron suspensiones celulares embriogénicas (SCE a partir de callo embriogénico obtenido de manos florales inmaduras masculinas, las cuales originaron abundantes embriones que regeneraron plantas. Hacia los tres meses de cultivo se detectaron embriones somáticos (ES primarios color blanco-crema en las manos florales de los nudos nueve a doce, contados a partir del ápice floral. Al cuarto mes estos ES primarios dieron origen al callo embriogénico, de color blanco crema, estructura granular, con abundantes ES torpedo en su periferia y con una organización celular en tres diferentes zonas. De este callo se cultivaron porciones pequeñas con ES torpedo en medio de multiplicación durante dos meses

  8. Karyotypic and 2C Nuclear DNA Size Instability in vitro Induced Off-Types of East African Highland Banana (Musa AAA East Africa

    Directory of Open Access Journals (Sweden)

    Theodosy Msogoya

    2008-01-01

    Full Text Available This study was conducted to determine chromosome number and 2C nuclear DNA content in tissue culture induced off-type banana (Musa AAA East Africa landrace Uganda with tolerance black sigatoka disease, susceptibility to water stress, sparsely black-blotched pseudostems, taller pseudostems, late fruit maturation, altered inflorescence and higher fruit dry matter content. The off-type banana appeared to have higher (p<0.05 frequency of 31 and 32 chromosomes at 15.1 and 13.6%, respectively. Conversely, the frequency of 31 and 32 chromosomes was 12.0 and 9.6% for the micropropagation (MP derived phenotypically normal plants and 11.8 and 9.5% for the Conventionally Propagation (CP derived plants with no tissue culture history. Moreover, the off-type banana had lower (p<0.05 leaf 2C nuclear DNA amount of 1.72 pg, whilst the MP and CP derived plants had 1.81 and 1.82 pg, respectively.

  9. Transcripts and MicroRNAs Responding to Salt Stress in Musa acuminata Colla (AAA Group cv. Berangan Roots.

    Directory of Open Access Journals (Sweden)

    Wan Sin Lee

    Full Text Available Physiological responses to stress are controlled by expression of a large number of genes, many of which are regulated by microRNAs. Since most banana cultivars are salt-sensitive, improved understanding of genetic regulation of salt induced stress responses in banana can support future crop management and improvement in the face of increasing soil salinity related to irrigation and climate change. In this study we focused on determining miRNA and their targets that respond to NaCl exposure and used transcriptome sequencing of RNA and small RNA from control and NaCl-treated banana roots to assemble a cultivar-specific reference transcriptome and identify orthologous and Musa-specific miRNA responding to salinity. We observed that, banana roots responded to salinity stress with changes in expression for a large number of genes (9.5% of 31,390 expressed unigenes and reduction in levels of many miRNA, including several novel miRNA and banana-specific miRNA-target pairs. Banana roots expressed a unique set of orthologous and Musa-specific miRNAs of which 59 respond to salt stress in a dose-dependent manner. Gene expression patterns of miRNA compared with those of their predicted mRNA targets indicated that a majority of the differentially expressed miRNAs were down-regulated in response to increased salinity, allowing increased expression of targets involved in diverse biological processes including stress signaling, stress defence, transport, cellular homeostasis, metabolism and other stress-related functions. This study may contribute to the understanding of gene regulation and abiotic stress response of roots and the high-throughput sequencing data sets generated may serve as important resources related to salt tolerance traits for functional genomic studies and genetic improvement in banana.

  10. Identification and expression analysis of four 14-3-3 genes during fruit ripening in banana (Musa acuminata L. AAA group, cv. Brazilian).

    Science.gov (United States)

    Li, Mei-Ying; Xu, Bi-Yu; Liu, Ju-Hua; Yang, Xiao-Liang; Zhang, Jian-Bin; Jia, Cai-Hong; Ren, Li-Cheng; Jin, Zhi-Qiang

    2012-02-01

    To investigate the regulation of 14-3-3 proteins in banana (Musa acuminata L. AAA group, cv. Brazilian) fruit postharvest ripening, four cDNAs encoding 14-3-3 proteins were isolated from banana and designated as Ma-14-3-3a, Ma-14-3-3c, Ma-14-3-3e, and Ma-14-3-3i, respectively. Amino acid sequence alignment showed that the four 14-3-3 proteins shared a highly conserved core structure and variable C-terminal as well as N-terminal regions with 14-3-3 proteins from other plant species. Phylogenetic analysis revealed that the four 14-3-3 genes belong to the non-ε groups. They were differentially and specifically expressed in various tissues. Real-time RT-PCR analysis indicated that these four genes function differentially during banana fruit postharvest ripening. Three genes, Ma-14-3-3a, Ma-14-3-3c, and Ma-14-3-3e, were significantly induced by exogenous ethylene treatment. However, gene function differed in naturally ripened fruits. Ethylene could induce Ma-14-3-3c expression during postharvest ripening, but expression patterns of Ma-14-3-3a and Ma-14-3-3e suggest that these two genes appear to be involved in regulating ethylene biosynthesis during fruit ripening. No obvious relationship emerged between Ma-14-3-3i expression in naturally ripened and 1-MCP (1-methylcyclopropene)-treated fruit groups during fruit ripening. These results indicate that the 14-3-3 proteins might be involved in various regulatory processes of banana fruit ripening. Further studies will mainly focus on revealing the detailed biological mechanisms of these four 14-3-3 genes in regulating banana fruit postharvest ripening. PMID:22009053

  11. EFEITO DO PERÍODO DE ARMAZENAMENTO SOBRE A BROTAÇÃO DE MUDAS DE BANANEIRA DO CULTIVAR NANICÃO (Musa acuminata AAA EFFECT OF STORAGE TIME IN THE BUDDING OF BANANA ROOTSTOCK CULTIVAR NANICÃO (Musa acuminata AAA

    Directory of Open Access Journals (Sweden)

    Ronaldo Veloso Naves

    2007-09-01

    Full Text Available

    A grande procura atual de mudas de bananeiras do cultivar Nanicão (Musa acuminata AAA em Goiás, principalmente na região do Mato Grosso Goiano, tem forçado o transporte de mudas a grandes distâncias. A maioria destas mudas são transportadas sob a forma de pedaços de rizoma com aproximadamente 1 Kg. Tem-se observado uma falha acima do previsto quando se utiliza este tipo de muda. O presente trabalho foi realizado para determinar o efeito do período de armazenamento sobre a brotação deste cultivar. Com base nos resultados concluiu-se que no período de armazenamento estudado (45 dias praticamente não houve diferença na brotação das mudas. Somente aquelas armazenadas por 5 (cinco semanas apresentaram inexplicavelmente um índice baixo de pegamento.

    Because of the tremendous current demand for Nanicão (Musa acuminata AAA banana shoots in the state of Goiás, notably in the “Mato Grosso Goiano” area, shoots have had to be imported from far away. The greater part of these shoots are transported in rootstalk form. Each rootstalk weighs approximately 1 Kg. A larger portion of these shoots than been anticipated, are defective. The present study was made to determine the effect of storage time on the budding of this particular banana rootstalk. The results of the experiment show that during the period of storage, forty-two (42 days, that was studied, there was almost no difference in budding among the rootstalks. Only those which were stored for five (5 weeks showed, unexplainably, a low budding index.

  12. Screening Fusarium wilt-resistant plants of Brazil banana (Musa spp., AAA) through EMS induced mutations from microcross sections cultural system

    International Nuclear Information System (INIS)

    Fusarium wilt is recognized as one of the most destructive diseases of banana worldwide. In this study, we screened Fusarium wilt-resistant plants of Brazil banana (Musa spp., AAA.) through EMS induced mutations from micro-cross sections cultural system. Micro-cross sections of pseudo stem of In vitro banana plantlets were treated with various concentrations of EMS for different duration, then were cultured in shoots induction medium. The results indicated that the survival index and the shoot forming index of the explants dropped with the increasing of EMS concentration and treatment duration. The optimal treatment for the concentration and duration was 300 mM and 60 min respectively. After the optimal treatment for 21 days, 2.2 regenerated shoots averagely could be produced from the explants of micro-cross sections, and the regenerated shoots were then cultured in shoots multiplying medium for 7 days. The stronger shoots were selected and transferred into roots medium for 4 weeks to obtain healthy regenerated plantlets. Hardened-regenerated plantlets were transplanted in green house for 2 months and 100 regenerated plants with vigorous root systems were selected for screening for Fusarium wilt resistance by using early screening technique. The initial disease symptom, yellowing in lower leaves, of susceptible plantlets could be observed after 2 weeks of inoculation with FOC race 4 and the extensive streaking on the most leaves was appeared after 2 months of inoculation. Only five plantlets survived and grew up healthily, which might be putative Fusarium wilt-resistant plants. Five sucks were selected from these 7-month-old putative resistant plants for screening the tolerance again and two suck- plants showed to be Fusarium wilt-resistant. Further studies on the Fusarium wilt tolerance of next generation of tissue cultural plants derived from these sucks and their genetic background are conducting. We concluded that the application of microcross sections of

  13. MANEJO DE LA SIMBIOSIS MICORRÍZICA ARBUSCULAR Y EL SUMINISTRO DE NUTRIENTES EN PLANTACIONES DE BANANO cv. ‘FHIA-18’ (Musa AAAB EN SUELO PARDO MULLIDO CARBONATADO

    Directory of Open Access Journals (Sweden)

    Jaime E. Simó González

    2015-01-01

    Full Text Available A partir de los beneficios de la simbiosis micorrízica arbuscular para la mayoría de las plantas y de regularidades en el manejo de cepas eficientes de hongos micorrízicos arbusculares (HMA, se incrementan los resultados positivos sobre su aplicación en diferentes cultivos. Teniendo en cuenta estos aspectos se realizó este trabajo, con el objetivo de evaluar la factibilidad de propuestas integradas de manejo de inoculantes micorrízicos y dosis complementarias de fertilización orgánico-mineral, para plantaciones del cultivar 'FHIA-18' en suelo Pardo mullido carbonatado. Se estudiaron dos esquemas para suministrar los nutrientes a las plantas, uno a base de fertilizantes minerales y otro a partir de fuentes orgánico-minerales, ambos en presencia o no de la inoculación de una cepa eficiente de HMA y durante los ciclos de planta madre y vástago-1 y 2, utilizando un diseño de bloques al azar con cuatro repeticiones. Se encontró una alta respuesta a la fertilización, siendo completamente equivalentes para el rendimiento y estado nutricional del banano, las dosis recomendadas tanto de fertilización mineral como órgano- mineral. La aplicación de HMA complementada con el 75 % de ambas fertilizaciones estudiadas, garantizaron rendimientos equivalentes y estados nutricionales similares entre sí y con los tratamientos que recibieron solo el 100 % de las dosis, pero con altos porcentajes de colonización micorrízica y producción de esporas. La inoculación de HMA realizada en el trasplante, mantuvo su efectividad y permanencia en los tres ciclos productivos evaluados. Las concentraciones foliares de potasio se asociaron directamente con el rendimiento y con la respuesta del banano a la inoculación con HMA .

  14. Deteminación de un índice de calidad del suelo en áreas productoras de banano (Musa x paradisiaca L.) de la vertiente del Pacifico de Panamá

    OpenAIRE

    Villarreal Nuñez, José Ezequiel

    2010-01-01

    El cultiu de la banana (Musa x paradisíaca L.) és un dels conreus agrícoles més importants en molts països tropicals i és el quart producte amb major volum d'exportació al món, només superat per l'arrós, el blat i el blat de moro (FAO, 2004). A Panamà, a pesar de l'aplicació de tècniques i insums d'alt cost, s'ha registrat una considerable reducció en la productivitat, Causa el canvi i deteriorament accelerat de les propietats físico-químiques i biològiques del sòl. Aquest treball es va reali...

  15. MECANISMO DE GELATINIZACIÓN DEL ALMIDÓN NATIVO DE BANANO EXPORTABLE DEL ECUADOR

    OpenAIRE

    Omar Martínez; Byron Lapo Calderón; Johnny Pérez Rodriguez; Cristhian Zambrano Cabrera; Favián Maza Valle

    2015-01-01

    El presente trabajo tuvo como objetivo fundamental estudiar el mecanismo de gelatinización del almidón nativo de banano de la especie Musa sapientum L. exportable del Ecuador. Se estudiaron las variedades Cavendish, Filipino, Valery y Orito, y a su vez, se estudió su potencial para ser añadido en alimentos que demanden características tecnológicas funcionales deseables como agentes espesantes, gelificantes y estabilizantes. También se determinó el tamaño, la forma de gránulos de almidón, temp...

  16. WHISKERS DE CELULOSA A PARTIR DE RESIDUOS AGROINDUSTRIALES DE BANANO: OBTENCIÓN Y CARACTERIZACIÓN

    OpenAIRE

    G.I. Bolio-López; Valadez-González, A.; Veleva, L.; A. Andreeva

    2011-01-01

    Debido al potencial que presentan los whiskers de celulosa (CW) como refuerzo en el diseño, procesamiento y producción de nanobiocompuestos, el objetivo de este estudio fue la obtención de whiskers a partir de residuos agroindustriales de banano (Musa cavendish). La metodología para la obtención de la celulosa incluyó procedimientos químicos, tales como hidrólisis ácida, cloración, extracción alcalina y blanqueamiento. Una doble hidrólisis acida controlada, (4N HCl y H2SO4 al 64%) y un proces...

  17. MECANISMO DE GELATINIZACIÓN DEL ALMIDÓN NATIVO DE BANANO EXPORTABLE DEL ECUADOR

    Directory of Open Access Journals (Sweden)

    Omar Martínez

    2015-05-01

    Full Text Available El presente trabajo tuvo como objetivo fundamental estudiar el mecanismo de gelatinización del almidón nativo de banano de la especie Musa sapientum L. exportable del Ecuador. Se estudiaron las variedades Cavendish, Filipino, Valery y Orito, y a su vez, se estudió su potencial para ser añadido en alimentos que demanden características tecnológicas funcionales deseables como agentes espesantes, gelificantes y estabilizantes. También se determinó el tamaño, la forma de gránulos de almidón, temperatura de gelatinización y viscosidad máxima; obteniéndose los siguientes valores: de 25 a 35 μm; 77,7 a 80 °C y de 259 a 270 UB, respectivamente. Según los resultados obtenidos, el almidón nativo de banano de las variedades estudiadas, puede ser incorporado en la elaboración de alimentos que demanden las características mencionadas.

  18. ABDOMINAL AORTIC ANEURYSM (AAA

    Directory of Open Access Journals (Sweden)

    Rajesh G

    2011-11-01

    Full Text Available AAA is defined as a distension of the infrarenal aorta by more than 50% (or 1.5 times compared with a corresponding healthy, aged and gender matched population. AAA afflicts 1 to 6 % of the general population aged more than 60 years and the incidence rises by approximately 0.15% annually. When the definition of a maximum external diameter ≥3 cm is used, the prevalence of AAA is upto 6 times greater in men than in women. AAAs are much more common than thoracic aortic aneurysms. Most common cause of AAA is atherosclerosis (95%. Less common causes include infectious or inflammatory origin or those associated with connective tissue disorders. Process of AAA formation is multifactorial. Other than the general risk factors for atherosclerosis, genetic predispo s iti on, aut o immunity and hemodynamic factors all play roles in its formation. AAA is 1.5 times more frequent in hypert ensive patients. Smokers have 8 times risk for developing AAA. The existence of familial aggre gation of AAA implicates genetic factors in the etiology of AAA. Women with AAA are more likely to have a positive family history of this disorder. Those with a family history of AAA have an increased risk of 30%, and their aneurysms tend to occur at a younger age and carry a greater risk of rupture than do sporadic aneurysms.

  19. Molecular Characterization of Somatic Mutation in Musa acuminata 'Red'

    International Nuclear Information System (INIS)

    Musa acuminata 'Red' (AAA) is a South Indian dessert banana cultivar (2n = 3x = 33) with a characteristic red color in the pseudostem, petiole and fruit peel. Red banana undergoes the process of somaclonal variation, producing the 'off types' Musa acuminata 'Green' cultivars. The frequency of the production of this 'green variant' is high during in vitro multiplication. In plants, anthocyanin pigments are assembled from phenyl alanine and acetyl CoA by the enzyme chalcone synthase (CHS). To understand the molecular mechanism for the somaclonal variation in Red banana, the chalcone synthase gene sequences were amplified using PCR products and sequences were compared with those of 'Green variants (AAA)', 'Dwarf Cavendish (AAA)' and diploid 'Pisang lilin' (AA). Sequence variations were observed only in amplified product from Red cultivar. Predicted amino acid sequences of the longest ORF indicated changes in seven amino acids such as arginine, glutamine, alanine, aspartic acid, isoleucine, phenylalanine and asparagine to serine, leucine, proline, alanine, valine, tyrosine and serine respectively. Musa acuminata cv. Red with anthocyanin content might have originated as a natural mutant, selected and maintained by vegetative propagation through generations. (author)

  20. Analysis of the distribution and structure of integrated Banana streak virus DNA in a range of Musa cultivars.

    Science.gov (United States)

    Geering, A D; Olszewski, N E; Dahal, G; Thomas, J E; Lockhart, B E

    2001-07-01

    Summary Banana streak virus strain OL (BSV-OL) commonly infects new Musa hybrids, and this infection is thought to arise de novo from integrated virus sequences present in the nuclear genome of the plant. Integrated DNA (Musa6+8 sequence) containing the whole genome of the virus has previously been cloned from cv. Obino l'Ewai (Musa AAB group), a parent of many of the hybrids. Using a Southern blot hybridization assay, we have examined the distribution and structure of integrated BSV-OL sequences in a range of Musa cultivars. For cv. Obino l'Ewai, almost every restriction fragment hybridizing to BSV-OL was predicted from the Musa6+8 sequence, suggesting that this is the predominant type of BSV-OL integrant in the genome. Furthermore, since only two junction fragments of Musa/BSV sequence were detected, and the Musa6+8 sequence is believed to be integrated as multiple copies in a tandem array, then the internal Musa spacer sequences must be highly conserved. Similarly sized restriction fragments were detected in four BB group cultivars, but not in six AA or AAA group cultivars, suggesting that the BSV-OL sequences are linked to the B-genome of Musa. We also provide evidence that cv. Williams (Musa AAA group) contains a distinct badnavirus integrant that is closely related to the 'dead' virus integrant previously characterized from Calcutta 4 (Musa acuminata ssp. burmannicoides). Our results suggest that the virus integrant from cv. Williams is linked to the A-genome, and the complexity of the hybridization patterns suggest multiple sites of integration and/or variation in sequence and structure of the integrants. PMID:20573008

  1. Fisiología de maduración postcosecha de banano variedad nanica (musa cavendishii)

    OpenAIRE

    Fanny Villamizar de Borrero

    2011-01-01

    Después de la cosecha, gran número de frutas llamadas climatéricas sufren profundas transformaciones bioquímicas que influyen directamente la calidad de esos productos. La respiración medida como la cantidad de CO2 producido por Kg de producto por hora, es el proceso metabólico más importante entre los demás que ocurren en las frutas después de la cosecha, siendo que su intensidad se refleja en la mayor o menor deterioración del producto, para una determinada condición de almacenamiento. La i...

  2. SELECCIÓN PARTICIPATIVA DE VARIEDADES Y EXPERIMENTACIÓN CAMPESINA EN AGRICULTURA URBANA EN EL CULTIVO DEL PLÁTANO (Musa spp.)

    OpenAIRE

    María de los A. Pino; Loracnis Hernández; María E. Dominí; Ramírez, A; Z. Terán; E. Calves

    2007-01-01

    El objetivo del trabajo fue probar si mediante la aplicación de la metodología de selección participativa de variedades y experimentación campesina en agricultura urbana en San José de las Lajas, provincia La Habana y Arroyo Naranjo, Ciudad de La Habana, se podía introducir, adoptar y diseminar biodiversidad vegetal en el cultivo de plátanos y bananos (Musa spp) en parcelas y patios. Los resultados mostraron las posibilidades que brindan las metodologías participativas para introducir y adop...

  3. Sobre musas y museos

    OpenAIRE

    Nápoli, Juan Tobías

    2000-01-01

    El arte de coleccionar, conservar, inventariar, exhibir e interpretar el caudal cultural de un pueblo se remonta a la antigüedad más remota. Sin embargo, la vinculación de estas prácticas con una institución específica (los museos), y la denominación de esta institución con un término relacionado con unas divinidades que constituyen un aspecto de la tradición cultural griega (las musas), tendrán una historia mucho más acotada.

  4. PROPAGACIÓN In Vitro DEL PLÁTANO MACHO (Musa sp. AAB) CLÓN SOBRINO CON LOS BIOESTIMULANTES CUBANOS BB-6 Y BIOSTAN COMO SUSTITUTOS DE LOS REGULADORES DEL CRECIMIENTO

    OpenAIRE

    E. Héctor; Torres, A; S. Algoe; M. Cabañas; López, A.

    2007-01-01

    La posibilidad de emplear bioestimulantes cubanos, como sustitutos de los reguladores del crecimiento convencionales, en la propagación masiva de los plátanos y bananos abriría una nueva perspectiva, para las biofábricas que actualmente se dedican a la multiplicación del género Musa en Cuba, e incrementaría las potencialidades exportables de los productos biotecnológicos cubanos. Se estudió el efecto de los bioestimulantes cubanos, BB-6 y Biostan, como sustitutos potenciales de los reguladore...

  5. Genetic Diversity in Musa acuminata Colla and Musa balbisiana Colla and some of their natural hybrids using AFLP Markers.

    Science.gov (United States)

    Ude, G.; Pillay, M.; Nwakanma, D.; Tenkouano, A.

    2002-06-01

    Genetic diversity and relationships were assessed in 28 accessions of Musa acuminata (AA) Colla and Musa balbisiana (BB) Colla, and some of their natural hybrids, using the amplified fragment length polymorphisms (AFLP) technique. Fifteen AFLP +3 primer pairs produced 527 polymorphic bands among the accessions. Neighbor-joining and principal co-ordinate (PCO) analyses using Jaccard's similarity coefficient produced four major clusters that closely corresponded with the genome composition of the accessions (AA, BB, AAB and ABB). The AFLP data distinguished between the wild diploid accessions and suggested new subspecies relationships in the M. acuminata complex that are different from those based on morphological data. The data suggested that there are three subspecies within the M. acuminata complex (ssp. burmannica Simmonds, malaccensis Simmonds, and microcarpa Simmonds). 'Tjau Lagada' (ssp. microcarpa), 'Truncata' [ssp truncata (Ridl.) Shepherd] and 'SF247' [ssp. banksii (F.Muell) Simmonds] clustered very closely with 'Gros Michel' and 'Km 5', indicating that more than one M. acuminata subspecies may be involved in the origin of triploid AAA bananas. 'Calcutta 4' (ssp. burmannicoides De Langhe & Devreux) and 'Long Tavoy' (ssp. burmannica) were closely related and could be together in the same subspecies. This study also showed that there is much more genetic diversity within M. balbisiana that was split into two groups: (1) 'I-63' and 'HND' and (2) 'Los Banos', 'MPL' (Montpellier), '10852', 'Singapuri', 'Etikehel', and 'Butohan 1' as the other. PMID:12582577

  6. Molecular characterization of somatic mutation in Musa acuminata 'Red'

    International Nuclear Information System (INIS)

    Musa acuminata 'Red' (AAA) is a South Indian dessert banana cultivar (2n = 3x = 33) with a characteristic red colour in the pseudostem, petiole and fruit peel. It is a popular edible variety grown extensively in India, Thailand, Sri Lanka, East Africa, West Indies, Myanmar and Continental America. Red banana undergoes the process of somaclonal variation, producing the 'off types' M. acuminata 'Green' cultivars. The frequency of the production of this 'green variant' is high during in vitro multiplication. In plants, anthocyanin pigments are assembled like all other flavanoids from two different streams of chemical raw materials in the cell. One stream involved the shikimate pathway to produce the amino acid phenyl alanine and the other stream produced 3 molecules of malonyl Co-A, a C3 unit from a C2 unit (acetyl Co A). These streams meet and are coupled together by the enzyme chalcone synthase (CHS), which forms an intermediate chalcone via a polyketide folding mechanism that is commonly found in plants. The chalcone is subsequently isomerized by the enzyme chalcone isomerase (CHI) to the prototype pigment naringenin - the precursor for flavanoids. More than five enzymes are required to synthesize anthocyanin pigments, each working in concert. Any even minor disruption in any of the mechanism of these enzymes by either genetic or environmental factors would halt anthocyanin production. To understand the molecular mechanism for the somaclonal variation in Red banana, the chalcone synthase gene sequences were amplified using PCR products cloned and sequences were compared with those of 'Green variants (AAA)', 'Dwarf Cavendish (AAA)' and diploid 'Pisang lilin' (AA). Sequence variations were observed only in amplified product from Red cultivar. Predicted amino acid sequences of the longest ORF indicated changes in seven amino acids such as arginine, glutamine, alanine, aspartic acid, isoleucine, phenylalanine and asparagine to serine, leucine, proline, alanine, valine

  7. The LHC at the AAAS

    CERN Multimedia

    CERN Bulletin

    2011-01-01

    The American Association for the Advancement of Science held its annual meeting in the Walter E. Washington Convention Center in Washington D.C. last week.   Veteran science writer Tim Radford introduces LHC scientists during a media briefing at the AAAS annual meeting. Left to right: Felicitas Pauss, Tom LeCompte, Yves Schutz and Nick Hadley. As the world’s largest popular science meeting, the AAAS meeting is a major event in the calendar of science journalists.  At this year’s LHC session, CERN’s coordinator for international relations, Felicitas Pauss, opened the discussion, paving the way for Tom LeCompte of ATLAS, Joe Incandela of CMS, Yves Schutz of ALICE and Monica Pepe-Altarelli of LHCb to report on the status of the first year’s analysis from their experiments.    

  8. Studi Pemakaian Tepung Pisang Ambon (Musa acuminata AAA) sebagai Anti-aging Dalam Sediaan Masker

    OpenAIRE

    Sinambela, Lisberia

    2016-01-01

    Background: Facial mask is cosmetic used as a skin care action, banana contains flavonoids, vitamins, and melatonin contained in banana flour efficacious as an antioxidants and smooth the skin, rejuvenate the skin, keeping the softness of the skin so the skin younger and fresher. Objective: This study was to formulate anti-aging mask with banana flour and to evaluate its anti-aging effect. Methods: Banana flour made by steaming banana 15 minutes, then thinly sliced, then dried under the...

  9. Effects of gamma radiation on banana 'nanica' (Musa sp., group AAA) irradiated in pre climacteric phase

    International Nuclear Information System (INIS)

    The present work verified the effect of gamma radiation on physical and chemical parameters of banana 'nanica', analyzing possible alterations on the period of conservation and the possibility of commercial irradiation aiming the exportation. The results had demonstrated that the radiations had not produced effect on pH and total acidity. However, the bananas of the 'control group' and those that had received 0,75 kGy, had presented greater maturation degree and, radiated with 0,30 kGy, had presented greater firmness. In accordance with the results of the organoleptic analysis, can be perceived that the bananas most mature, especially of the 'control group', had had greater acceptance. The bananas of treatments 0,30 and 0,60 kGy had had minors notes for presenting minor maturation stadium. Knowing that the irradiation in adequate dose and fruits of good quality brings benefits to the storage and the process of exportation, we conclude that the dose most appropriate for the control of the maturation of the 'nanica' banana is 0,30 kGy. (author)

  10. Gamma irradiated variants of banana cultivar Giant Cavendish (AAA) and their characterization using RAPD markers

    International Nuclear Information System (INIS)

    Bananas and plantains are one of the most important fruit crops and a staple food for millions in the tropical and subtropical countries. Owing to the constraints of vegetative propagation, sterility and triploid nature of most of the cultivars, mutation breeding and biotechnological methods are useful tools for developing cultivars resistant to diseases and pests. Herein, multiple shoot cultures of banana Giant Cavendish (Musa spp. AAA group) were gamma-irradiated (5, 10 and 30 Gy). Field evaluation of these exhibited variation for several agronomic traits including dwarf stature and early flowering. The selections are now being studied for stability. Molecular analysis using RAPD markers indicated polymorphism and some of the dwarf selections were analyzed using dwarf-specific SCAR marker confirming their dwarf behavior. The results suggest that the gamma irradiation is useful for the isolation of agronomic variants in Cavendish bananas (author)

  11. Insights into the Musa genome: Syntenic relationships to rice and between Musa species

    Directory of Open Access Journals (Sweden)

    Althoff Ryan

    2008-01-01

    Full Text Available Abstract Background Musa species (Zingiberaceae, Zingiberales including bananas and plantains are collectively the fourth most important crop in developing countries. Knowledge concerning Musa genome structure and the origin of distinct cultivars has greatly increased over the last few years. Until now, however, no large-scale analyses of Musa genomic sequence have been conducted. This study compares genomic sequence in two Musa species with orthologous regions in the rice genome. Results We produced 1.4 Mb of Musa sequence from 13 BAC clones, annotated and analyzed them along with 4 previously sequenced BACs. The 443 predicted genes revealed that Zingiberales genes share GC content and distribution characteristics with eudicot and Poaceae genomes. Comparison with rice revealed microsynteny regions that have persisted since the divergence of the Commelinid orders Poales and Zingiberales at least 117 Mya. The previously hypothesized large-scale duplication event in the common ancestor of major cereal lineages within the Poaceae was verified. The divergence time distributions for Musa-Zingiber (Zingiberaceae, Zingiberales orthologs and paralogs provide strong evidence for a large-scale duplication event in the Musa lineage after its divergence from the Zingiberaceae approximately 61 Mya. Comparisons of genomic regions from M. acuminata and M. balbisiana revealed highly conserved genome structure, and indicated that these genomes diverged circa 4.6 Mya. Conclusion These results point to the utility of comparative analyses between distantly-related monocot species such as rice and Musa for improving our understanding of monocot genome evolution. Sequencing the genome of M. acuminata would provide a strong foundation for comparative genomics in the monocots. In addition a genome sequence would aid genomic and genetic analyses of cultivated Musa polyploid genotypes in research aimed at localizing and cloning genes controlling important agronomic

  12. The role of mutation techniques and genomics for banana and plantain (Musa spp.), major staple crops in the tropics

    International Nuclear Information System (INIS)

    Bananas and plantains are produced in 126 countries throughout the tropics and sub-tropics, on an area of 12.5 million acres (5Mha), with annual production exceeding 100 million tons. Dessert bananas exported as one of the most widely eaten of the 'five-a-day fruits' in the industrialized countries, with a value of $US 4.86 billion and underpinning the economy of many developing countries, account for 15% of this total. The remainder provide a staple food and major source of income for as many as 400 million people in developing countries. The banana export industry relies on genetically closely related clones of the Cavendish sub-group (sterile triploids, AAA). Though high-yielding, this sub-group of cultivars is extremely vulnerable to biotic and abiotic stresses and, due to high levels of sterility, is very hard to improve though classical breeding. A high quality genomic sequence of Musa has immediate application in assisting localization and identification of genes and alleles related to biotic (pest and disease) stresses, to abiotic (environmental, including drought, flooding, wind and salinity) stresses and to fruit quality (including post-harvest processes and nutrition). The latest information on activities and resources developed by the Global Musa Genomics Consortium will be presented. It will be demonstrated that by organizing the Global Musa Genomics Consortium (currently comprising 37 member institutions from 24 countries), duplication of effort can be minimized and the results of Musa genomics research are rapidly made accessible to taxonomists, breeders and the biotechnology community. (author)

  13. Sequence analysis of the AAA protein family.

    OpenAIRE

    Beyer, A.

    1997-01-01

    The AAA protein family, a recently recognized group of Walker-type ATPases, has been subjected to an extensive sequence analysis. Multiple sequence alignments revealed the existence of a region of sequence similarity, the so-called AAA cassette. The borders of this cassette were localized and within it, three boxes of a high degree of conservation were identified. Two of these boxes could be assigned to substantial parts of the ATP binding site (namely, to Walker motifs A and B); the third ma...

  14. LMIP/AAA: Local Authentication, Authorization and Accounting (AAA) Protocol for Mobile IP

    Science.gov (United States)

    Chenait, Manel

    Mobile IP represents a simple and scalable global mobility solution. However, it inhibits various vulnerabilities to malicious attacks and, therefore, requires the integration of appropriate security services. In this paper, we discuss two authentication schemes suggested for Mobile IP: standard authentication and Mobile IP/AAA authentication. In order to provide Mobile IP roaming services including identity verication, we propose an improvement to Mobile/AAA authentication scheme by applying a local politic key management in each domain, hence we reduce hando latency by avoiding the involvement of AAA infrastructure during mobile node roaming.

  15. SELECCIÓN PARTICIPATIVA DE VARIEDADES Y EXPERIMENTACIÓN CAMPESINA EN AGRICULTURA URBANA EN EL CULTIVO DEL PLÁTANO (Musa spp.

    Directory of Open Access Journals (Sweden)

    María de los A. Pino

    2007-01-01

    Full Text Available El objetivo del trabajo fue probar si mediante la aplicación de la metodología de selección participativa de variedades y experimentación campesina en agricultura urbana en San José de las Lajas, provincia La Habana y Arroyo Naranjo, Ciudad de La Habana, se podía introducir, adoptar y diseminar biodiversidad vegetal en el cultivo de plátanos y bananos (Musa spp en parcelas y patios. Los resultados mostraron las posibilidades que brindan las metodologías participativas para introducir y adoptar materiales a partir de una feria de agrobiodiversidad de frutos y las diferencias en la selección de los materiales, en función de los criterios de selección y los contextos particulares de cada municipio. Estos resultados también brindan las primeras evidencias concretas de este tipo de experiencias en un entorno urbano y para este tipo de cultivo.

  16. Autocatalytic Processing of m-AAA Protease Subunits in Mitochondria

    OpenAIRE

    Koppen, Mirko; Bonn, Florian; Ehses, Sarah; Langer, Thomas

    2009-01-01

    m-AAA proteases are ATP-dependent proteolytic machines in the inner membrane of mitochondria which are crucial for the maintenance of mitochondrial activities. Conserved nuclear-encoded subunits, termed paraplegin, Afg3l1, and Afg3l2, form various isoenzymes differing in their subunit composition in mammalian mitochondria. Mutations in different m-AAA protease subunits are associated with distinct neuronal disorders in human. However, the biogenesis of m-AAA protease complexes or of individua...

  17. Regulatory Circuits of the AAA+ Disaggregase Hsp104

    OpenAIRE

    Franzmann, Titus M.; Czekalla, Anna; Walter, Stefan G.

    2011-01-01

    Yeast Hsp104 is an AAA+ chaperone that rescues proteins from the aggregated state. Six protomers associate to form the functional hexamer. Each protomer contains two AAA+ modules, NBD1 and NBD2. Hsp104 converts energy provided by ATP into mechanical force used to thread polypeptides through its axial channel, thereby disrupting protein aggregates. But how the action of its 12 AAA+ domains is co-ordinated to catalyze disaggregation remained unexplained. Here, we identify a sophisticated allost...

  18. Results of a Musa mapping project

    International Nuclear Information System (INIS)

    A completed map, based on two selfed progenies from two banana cultivars (M53 and SFB5) is presented (roughly 1200 cM). More than three hundred markers are linked in 11 linkage groups representing the genome (2n=22) of Musa acuminata. Roughly one third of the markers are co-dominant restriction fragment polymorphisms (RFLPs; one hundred) or micro satellites (thirty). Two thirds of the markers are dominant amplified fragment length polymorphisms (AFLPs; 10% could be considered to be co-dominant). The mean linkage distance is 3 cM, but marker density still should be increased on a couple of linkage groups. Particularities for a mapping job in banana are discussed. Due to Musa acuminata sub-species specific translocations, up to 36% of all the markers tested show important segregation distortions. The need for a cooperative mapping initiative based on a proposed ''frame-map'' harbouring evenly spaced co-dominant ''anchor'' markers is proposed. CIRAD has published 45 sequence tagged micro satellite sites (STMS) in the EMBL database which are accessible at: ''http://www.ebi.ac.uk/'' using the keywords LAGODA and MICROSATELLITE (EMBL accessions X87258 to X87265, X90740 to X90750 and Z85950 to Z85977). (author)

  19. Chemical and physical characterization of Musa sepientum and Musa balbisiana fibers of banana tree

    International Nuclear Information System (INIS)

    This study aimed to characterize the fibers of cavendish and silver banana trunks (Musa sepientum and Musa balbisiana, respectively) concerning their density, lignin and moisture contents, and chemical structure by using the techniques of infrared spectroscopy and low field solid state nuclear magnetic resonance, NMR. From NMR analysis, it was possible to observe the morphological differences between cavendish and silver types of banana fibers. FTIR technique did not allow the observation of any important difference in the banana fibers spectra. The cavendish banana fiber showed higher moisture and lignin contents than the silver banana fiber The NMR technique showed that relaxation times for silver banana fiber were higher than those for cavendish banana fiber, which can be credited to the lower moisture content values found in the silver fibers. (author)

  20. A platform for efficient genotyping in Musa using microsatellite markers

    OpenAIRE

    Christelová, Pavla; Valárik, Miroslav; Hřibová, Eva; Van den Houwe, Ines; Channelière, Stéphanie; Roux, Nicolas; Doležel, Jaroslav

    2011-01-01

    Background and aims Bananas and plantains (Musa spp.) are one of the major fruit crops worldwide with acknowledged importance as a staple food for millions of people. The rich genetic diversity of this crop is, however, endangered by diseases, adverse environmental conditions and changed farming practices, and the need for its characterization and preservation is urgent. With the aim of providing a simple and robust approach for molecular characterization of Musa species, we developed an opti...

  1. Optimatization of transient transformation methods to study gene expression in Musa acuminata (AAA group) cultivar Ambon Lumut

    Science.gov (United States)

    Prayuni, Kinasih; Dwivany, Fenny M.

    2015-09-01

    Banana is classified as a climateric fruit, whose ripening is regulated by ethylene. Ethylene is synthesized from ACC (1-aminocyclopropane-1-carboxylic acid) by ACC oxidase enzyme which is encoded by ACO gene. Controling an important gene expression in ethylene biosynthesis pathway has became a target to delay the ripening process. Therefore in the previous study we have designed a MaACO-RNAi construct to control MaACO gene expression. In this research, we study the effectiveness of different transient transformation methods to deliver the construct. Direct injection, with or no vaccum infiltration methods were used to deliver MaACO-RNAi construct. All of the methods succesfully deliver the construct into banana fruits based on RT-PCR result.

  2. Agronomical and molecular factors influencing bananas (Musa acuminata, AAA, cv ‘Grande-Naine’) susceptibility to crown rot disease

    OpenAIRE

    Lassois, Ludivine

    2009-01-01

    Crown rot affects export bananas in all producing countries and is considered to be one of the main export banana post-harvest disease. Variations are observed in the expression of crown rot symptoms. An original approach of the disease is proposed and consists on presenting the fruit quality potential at harvest as a key factor in crown rot development. This potential develops during growth of bananas in the field and depends on a physiological and a parasitical component. The...

  3. AAAS Communicating Science Program: Reflections on Evaluation

    Science.gov (United States)

    Braha, J.

    2015-12-01

    The AAAS Center for Public Engagement (Center) with science builds capacity for scientists to engage public audiences by fostering collaboration among natural or physical scientists, communication researchers, and public engagement practitioners. The recently launched Leshner Leadership Institute empowers cohorts of mid-career scientists to lead public engagement by supporting their networks of scientists, researchers, and practitioners. The Center works closely with social scientists whose research addresses science communication and public engagement with science to ensure that the Communicating Science training program builds on empirical evidence to inform best practices. Researchers ( Besley, Dudo, & Storkdieck 2015) have helped Center staff and an external evaluator develop pan instrument that measures progress towards goals that are suggested by the researcher, including internal efficacy (increasing scientists' communication skills and confidence in their ability to engage with the public) and external efficacy (scientists' confidence in engagement methods). Evaluation results from one year of the Communicating Science program suggest that the model of training yields positive results that support scientists in the area that should lead to greater engagement. This talk will explore the model for training, which provides a context for strategic communication, as well as the practical factors, such as time, access to public engagement practitioners, and technical skill, that seems to contribute to increased willingness to engage with public audiences. The evaluation program results suggest willingness by training participants to engage directly or to take preliminary steps towards engagement. In the evaluation results, 38% of trained scientists reported time as a barrier to engagement; 35% reported concern that engagement would distract from their work as a barrier. AAAS works to improve practitioner-researcher-scientist networks to overcome such barriers.

  4. The Complete Chloroplast Genome of Banana (Musa acuminata, Zingiberales): Insight into Plastid Monocotyledon Evolution

    OpenAIRE

    Guillaume Martin; Franc-Christophe Baurens; Céline Cardi; Jean-Marc Aury; Angélique D'Hont

    2013-01-01

    BACKGROUND: Banana (genus Musa) is a crop of major economic importance worldwide. It is a monocotyledonous member of the Zingiberales, a sister group of the widely studied Poales. Most cultivated bananas are natural Musa inter-(sub-)specific triploid hybrids. A Musa acuminata reference nuclear genome sequence was recently produced based on sequencing of genomic DNA enriched in nucleus. METHODOLOGY/PRINCIPAL FINDINGS: The Musa acuminata chloroplast genome was assembled with chloroplast reads e...

  5. Positron emission tomography (PET) evaluation of abdominal aortic aneurysm (AAA)

    OpenAIRE

    SakalihasanN, Natzi; Van Damme, Hendrik; Gomez, P.; RIGO, PIERRE; Lapiere, C. M.; Nusgens, Betty; Limet, Raymond

    2002-01-01

    Background: aneurysmal disease is associated with all inflammatory Cell infiltrate and enzymatic degradation of the vessel wall. Aim of the study: to detect increased metabolic activity in abdominal aortic aneurysms (AAA) by means of positron emission tomography (PET-imaging). Study design: twenty-six patients with AAA underwent PET-imaging Results: in tell patients, PET-imaging revealed increased, fluoro-deoxy-glucose (18-FDG) uptake at the level of the aneurysm. Patients with positive PET-i...

  6. Improvement of Musa through biotechnology and mutation breeding

    International Nuclear Information System (INIS)

    Edible Musa, i.e. bananas, plantains and cooking bananas, are mostly sterile polyploids with a reproductive system that is extremely difficult to manipulate. The presently used breeding methods based on crossing of near-sterile triploids with semi-wild diploids is time consuming and laborious. The cultivation of bananas in many regions is seriously threatened by several diseases caused by pathogenic fungi (Mycosphaerella, Fusarium), bacteria (Pseudomonas), viruses (esp. bunchy top virus) and nematodes. Breeding for resistance requires new strategies and the implementation of biotechnology. The current applications of biotechnology in Musa improvement include; (1) embryo culture for hybridization, (2) shoot-tip culture in micropropagation, (3) in vitro mutation induction, (4) somatic embryogenesis and somaclonal variation, (5) in vitro screening and selection, (6) artificial seed production and (7) DNA fingerprinting applied to genomic analysis. The results and ongoing projects of the Joint FAO/IAEA Programme are: (1) development of in vitro mutation systems in shoot-tip cultures, (2) development of a mutant clone with superior performance to the original Cavendish type ''Grain Nain'', (3) somatic embryogenesis in cell suspensions of Musa, (4) protocols for the isolation and short term culture of protoplasts, (5) the study of somaclonal variation for the genetic improvement of banana, (6) encapsulation of somatic embryos for artificial seed production, (7) selection techniques and nursery screening techniques for disease resistance breeding of Musa and, (8) protein electrophoresis and DNA fingerprinting for genomic characterization of different Musa clones. 11 refs, 1 fig

  7. Inhibitors of the AAA+ Chaperone p97

    Directory of Open Access Journals (Sweden)

    Eli Chapman

    2015-02-01

    Full Text Available It is remarkable that a pathway as ubiquitous as protein quality control can be targeted to treat cancer. Bortezomib, an inhibitor of the proteasome, was first approved by the US Food and Drug Administration (FDA more than 10 years ago to treat refractory myeloma and later extended to lymphoma. Its use has increased the survival rate of myeloma patients by as much as three years. This success was followed with the recent accelerated approval of the natural product derived proteasome inhibitor carfilzomib (Kyprolis®, which is used to treat patients with bortezomib-resistant multiple myeloma. The success of these two drugs has validated protein quality control as a viable target to fight select cancers, but begs the question why are proteasome inhibitors limited to lymphoma and myeloma? More recently, these limitations have encouraged the search for additional targets within the protein quality control system that might offer heightened cancer cell specificity, enhanced clinical utility, a lower rate of resistance, reduced toxicity, and mitigated side effects. One promising target is p97, an ATPase associated with various cellular activities (AAA+ chaperone. p97 figures prominently in protein quality control as well as serving a variety of other cellular functions associated with cancer. More than a decade ago, it was determined that up-regulation of p97 in many forms of cancer correlates with a poor clinical outcome. Since these initial discoveries, a mechanistic explanation for this observation has been partially illuminated, but details are lacking. Understandably, given this clinical correlation, myriad roles within the cell, and its importance in protein quality control, p97 has emerged as a potential therapeutic target. This review provides an overview of efforts towards the discovery of small molecule inhibitors of p97, offering a synopsis of efforts that parallel the excellent reviews that currently exist on p97 structure, function, and

  8. Post harvest changes of banana prata (Musa acuminata x Musa balbisiana group AAB) γ-irradiated

    International Nuclear Information System (INIS)

    Pre-climateric bananas (Musa acuminata x Musa balbisiana [AAB group] cv. Prata) were stored without treatment (no radiation) or were exposed at 0.25 and 0.50 kGy of γ -irradiation, with a dose rate of 1 kGy.h -1 before storage. The fruits were analysed for CO2 and ethylene production during maturation and pulp-to-peel ratio, starch, total soluble sugars, pectins, pectin methyl esterase and poly galacturonase activities on 6 colour score of peel. The radiation dose/peel-colour interaction affected the pulp-to-peel ratio significantly, while the fruits subjected at 0.25 Gy had the highest increase on the colour scores 5, 6, and 7. A predictable increase in the conversion of starch to sugar took place during maturation and a delaying on the soluble sugar accumulation was noted on the fruits submitted to 0.50 kGy, in the 6 and 7 colour scores only. A decrease on the total pectin content and a trend of a higher solubilization of pectins was observed in the fruits γ-irradiated at 0.50 kGy. Higher pectin methyl esterase activities were exhibited by irradiated fruits, although the γ-irradiation has suppressed the poly galacturonase activity throughout the maturation period. (author). 110 refs., 11 figs., 5 tabs

  9. Atividade antiviral de Musa acuminata Colla, Musaceae Antiviral activity of Musa acuminata Colla, Musaceae

    Directory of Open Access Journals (Sweden)

    Fernanda Otaviano Martins

    2009-09-01

    Full Text Available O presente trabalho avalia a atividade antiviral de extratos e frações de Musa acuminata Colla, Musaceae, coletada em duas regiões do Estado do Rio de Janeiro (Petrópolis e Santo Antônio de Pádua. As inflorescências de M. acuminata apresentaram excelente atividade para os dois vírus avaliados: herpesvírus simples humano tipo 1 e herpesvírus simples humano tipo 2, ambos resistentes ao Aciclovir. Os resultados indicam que os extratos de M. acuminata testados podem constituir alvo potencial para uso em terapias antivirais.This study evaluates the antiviral activity of extracts and fractions of Musa acuminata Colla collected in two regions of Rio de Janeiro State (Petrópolis and Santo Antônio de Pádua. The inflorescences of M. acuminata showed excellent activity for the two virus evaluated: simple human herpesvirus type 1 and simple human herpesvirus type 2, both resistant to Acyclovir. The results indicate that the tested extracts of M. acuminata can be potential target for use in antiviral therapy.

  10. GROSOR DEL FRUTO DE LA ÚLTIMA Y SEGUNDA MANO COMO CRITERIO DE COSECHA EN BANANO

    Directory of Open Access Journals (Sweden)

    Alfonso Vargas-Calvo

    2012-01-01

    Full Text Available Grosor del fruto de la última y segunda mano como criterio de cosecha en banano. El objetivo de este trabajo fue determinar la relación entre el grosor del fruto central de las manos última y segunda, como criterio de cosecha en el banano. Las mediciones se efectuaron semanalmente (mm de la segunda a la décima semana después de la fl oración y fueron transformadas a treintaidosavos de pulgada (tdp donde 1 tdp= 0,7914 mm, para la generación de ecuaciones de regresión para ambas manos, considerando, o ignorando, el tamaño del racimo. El grosor del fruto central de la última mano fue estimado a partir del grosor del fruto central de la segunda mano, y viceversa para el grosor del fruto de la segunda mano. Los valores de grosor del fruto central de la última mano (valores Y obtenidos aplicando los grosores mínimos preestablecidos de cosecha para el fruto central de la segunda mano (valores x: 43; 44 y 45 tdp fueron superiores a 40 tdp, independientemente del tamaño del racimo, e inclusive superó en la mayoría de las veces, a 41 tdp. Inversamente, aplicando los valores mínimos de grosor de la última mano, predefi nidos para la cosecha (valores x: 39; 40 y 41 tdp, se obtuvieron valores de grosor del fruto central de la segunda mano (valores Y, que no superaron el valor de 43 tdp excepto para x=41 en los racimos de siete a nueve manos. Los racimos cosechados con la exigencia mínima de empaque aplicada para la última mano, tuvieron menos peso, por estar conformados de frutos con menor grosor, que aquellos que se cosecharían mediante la medición en la segunda mano.

  11. Alimentación de bovinos con ensilado de mezclas de banano de rechazo y ráquis en diferentes proporciones

    Directory of Open Access Journals (Sweden)

    J. C. De la Cruz-Hernández

    2006-01-01

    Full Text Available Con el propósito de evaluar el comportamiento productivo de bovinos consumiendo ensilado de mezclas de banano de rechazo como fruta y ráquis en diferentes proporciones, se realizó una prueba de alimentación con 12 bovinos cebú comercial, machos, enteros, con un peso promedio de 168±17 kg, a los que se les asignó, de manera aleatoria, cada uno de los siguientes tratamientos: I ensilado con 50% banano y 50% ráquis; II ensilado con 75% banano y 25% ráquis; y III un grupo testigo con zacate Taiwán (Pennisetum purpureum. Los animales recibieron, además, 1.1 kg de pasta de soya como fuente proteica y 50 g de una premezcla comercial de minerales y sal común. La prueba tuvo una duración de 120 días, más un tiempo de adaptación de 15, periodo en el que las dietas se ofrecieron a libertad. Los animales fueron pesados cada 30 días y se les llevó diariamente el control de consumo de alimento. Las mejores (P0.05 entre ellos. El consumo de alimento base húmeda (CABH no presentó diferencias significativas (P>0.05 entre tratamientos; sin embargo, el consumo de alimento base seca (CABS fue mayor (P<0.05 en el tratamiento ensilado de banano-ráquis 75:25 comparado con el de 50:50 (6.433 vs. 4.308 kg. El consumo de materia seca/100 kg de peso vivo fue mayor (P<0.05 para los animales que consumieron zacate Taiwán y ensilado de banano-ráquis 75:25 (2.334 y 1.990, comparados con los del ensilado 50:50 (1.413. El consumo de proteína cruda en kg fue mayor (P<0.05 para los animales alimentados con zacate Taiwán. Porcentualmente, el consumo de proteína cruda fue mayor (P<0.05 en los animales que consumieron zacate Taiwán y ensilado de banano-ráquis 50:50 (16.99 y 17.36 %. La mayor eficiencia alimenticia (P<0.05 la obtuvieron los animales que consumieron el ensilado de banano-ráquis 50:50. El análisis económico de este sistema de alimentación nos indica que se obtuvieron las mayores ganancias con el uso de los ensilados, siendo el mejor

  12. Efectos Ambientales y Socieconómicos de los Procesos Productivos del Café y del Banano: Una Mirada Multifactorial

    Directory of Open Access Journals (Sweden)

    José Alfredo Vásquez

    2006-10-01

    Full Text Available Durante el siglo XX y hasta la actualidad la produccion del sector agricola y en particular de los subsectores de cafe y banano han jugado un importante papel en la economia antioquena, tanto en la generacion de empleo y divisas, como en la construccion de una cultura asociada a ambos sectores. Las investigaciones realizadas por diversas instituciones en ambos subsectores si bien han orientado la introduccion de mejoras en la produccion, han dejado de lado un analisis comparativo de ambos subsectores con miras a la identificacion politicas propendan por el mejoramiento conjunto de ambos procesos productivos en aspectos de indole socioeconomico, y ambiental. El articulo presenta los resultados y conclusiones de un analisis comparativo de los procesos productivos del cafe y el banano en el departamento de Antioquia, desde los aspectos socioeconomico y ambiental, haciendo uso de una metodologia de analisis factorial (cuantitativo.

  13. Producción de etanol a partir de la cáscara de banano y de almidón de yuca

    OpenAIRE

    JOHN F. MONSALVE G.; VICTORIA ISABEL MEDINA DE PEREZ; ANGELA ADRIANA RUIZ COLORADO

    2006-01-01

    En este trabajo se evaluó la hidrólisis ácida del almidón presente en yuca y de la celulosa presente en cáscara de banano y su posterior fermentación a etanol, se ajustaron los medios de fermentación para los microorganismos Saccharomyces cerevisiae NRRL Y-2034 y Zymomonas mobilis CP4. Se caracterizó la cáscara de banano, la cual posee un contenido de almidón, celulosa y hemicelulosa que representan más del 80 % de la cáscara ameritando el estudio de ésta como fuente de carbono. La hidróli...

  14. Focus on the Musa collection: Ploidy levels revealed

    Czech Academy of Sciences Publication Activity Database

    Kubaláková, Marie; Doležel, Jaroslav; Van den Houwe, I.; Roux, N.; Swennen, R.

    2005-01-01

    Roč. 14, - (2005), s. 34-36. ISSN 1023-0076 R&D Projects: GA AV ČR IAA6038204 Grant ostatní: IAEA res. contract No. 12230 Institutional research plan: CEZ:AV0Z50380511 Keywords : Musa * ploidy * flow cytometry Subject RIV: EB - Genetics ; Molecular Biology

  15. Analysis of Musa genome using flow cytometry and molecular cytogenetics

    Czech Academy of Sciences Publication Activity Database

    Doležel, Jaroslav; Valárik, Miroslav; Vrána, Jan; Šafář, Jan; Hřibová, Eva; Gasmanová, Nikol; Van den Howe, I.; Doleželová, Marie; Swennen, R.; Šimková, Hana

    2002. s. 16-17. [ FAO /IAEA Research Co-ordination Meeting on Cellular Biology and Biotechnology Including Mutation Techniques for Creation of New Useful Banana Genotypes /4./. 24.09.2002-28.09.2002, Leuven] Institutional research plan: CEZ:AV0Z5038910 Keywords : flow cytometry * molecular cytogenetics * Musa spp Subject RIV: EB - Genetics ; Molecular Biology

  16. The Adult Asperger Assessment (AAA): A Diagnostic Method

    Science.gov (United States)

    Baron-Cohen, Simon; Wheelwright, Sally; Robinson, Janine; Woodbury-Smith, Marc

    2005-01-01

    At the present time there are a large number of adults who have "suspected" Asperger syndrome (AS). In this paper we describe a new instrument, the Adult Asperger Assessment (AAA), developed in our clinic for adults with AS. The need for a new instrument relevant to the diagnosis of AS in adulthood arises because existing instruments are designed…

  17. A structural analysis of the AAA+ domains in Saccharomyces cerevisiae cytoplasmic dynein

    OpenAIRE

    Gleave, Emma S.; Schmidt, Helgo; Carter, Andrew P.

    2014-01-01

    Dyneins are large protein complexes that act as microtubule based molecular motors. The dynein heavy chain contains a motor domain which is a member of the AAA+ protein family (ATPases Associated with diverse cellular Activities). Proteins of the AAA+ family show a diverse range of functionalities, but share a related core AAA+ domain, which often assembles into hexameric rings. Dynein is unusual because it has all six AAA+ domains linked together, in one long polypeptide. The dynein motor do...

  18. Increased levels of thioredoxin in patients with abdominal aortic aneurysms (AAAs). A potential link of oxidative stress with AAA evolution

    DEFF Research Database (Denmark)

    Martinez-Pinna, R; Lindholt, Jes S.; Blanco-Colio, L M;

    2010-01-01

    Oxidative stress is a main mechanism involved in vascular pathologies. Increased thioredoxin (TRX) levels have been observed in several oxidative stress-associated cardiovascular diseases. We aim to test the potential role of TRX as a biomarker of oxidative stress in abdominal aortic aneurysm (AAA)....

  19. MORPHOLOGICAL CHARACTERIZATION OF MALAYSIAN WILD BANANA MUSA ACUMINATA

    Directory of Open Access Journals (Sweden)

    MUHAMMAD ASIF JAVED

    2002-01-01

    Full Text Available Fourteen populations of Musa acuminata ranging from populations in the lowlands of northern (ssp. siamea to central Malaysian region (ssp. malaccensis and highland banana (ssp. truncata were characterized based on chromosome number and 46 morphological characters. A large amount of variation was observed within the populations. However, only highland bananas appeared morphologically distinct. Lowland populations both from northern and central Malaysia were found to be overlapping and no distinguishing pattern was observed. The morphological characters found variable within these populations were related to developmental changes and mutations. The results ob tained in this study were not revolutionary. However, the survey of a large number of characters treated with multivariate techniques further sharpened the existing groupings of the Musa acuminata subspecies.

  20. MORPHOLOGICAL CHARACTERIZATION OF MALAYSIAN WILD BANANA MUSA ACUMINATA

    OpenAIRE

    MUHAMMAD ASIF JAVED; MAK CHAI; ROFINA YASMIN OTHMAN

    2002-01-01

    Fourteen populations of Musa acuminata ranging from populations in the lowlands of northern (ssp. siamea) to central Malaysian region (ssp. malaccensis) and highland banana (ssp. truncata) were characterized based on chromosome number and 46 morphological characters. A large amount of variation was observed within the populations. However, only highland bananas appeared morphologically distinct. Lowland populations both from northern and central Malaysia were found to be overlapping a...

  1. A structural analysis of the AAA+ domains in Saccharomyces cerevisiae cytoplasmic dynein.

    Science.gov (United States)

    Gleave, Emma S; Schmidt, Helgo; Carter, Andrew P

    2014-06-01

    Dyneins are large protein complexes that act as microtubule based molecular motors. The dynein heavy chain contains a motor domain which is a member of the AAA+ protein family (ATPases Associated with diverse cellular Activities). Proteins of the AAA+ family show a diverse range of functionalities, but share a related core AAA+ domain, which often assembles into hexameric rings. Dynein is unusual because it has all six AAA+ domains linked together, in one long polypeptide. The dynein motor domain generates movement by coupling ATP driven conformational changes in the AAA+ ring to the swing of a motile element called the linker. Dynein binds to its microtubule track via a long antiparallel coiled-coil stalk that emanates from the AAA+ ring. Recently the first high resolution structures of the dynein motor domain were published. Here we provide a detailed structural analysis of the six AAA+ domains using our Saccharomycescerevisiae crystal structure. We describe how structural similarities in the dynein AAA+ domains suggest they share a common evolutionary origin. We analyse how the different AAA+ domains have diverged from each other. We discuss how this is related to the function of dynein as a motor protein and how the AAA+ domains of dynein compare to those of other AAA+ proteins. PMID:24680784

  2. Ocenenie skupiny AAA Auto Group N.V.

    OpenAIRE

    Tatár, Dávid

    2012-01-01

    The objective of diploma thesis is about to define a value of a international group AAA Auto Group N. V. which operates on the czech, slovak and russian market with used cars and analysis of an ability to increase shareholder's value in the future. It is structured as an expert opinion. It defines purpose, subject and exact date of valuation, valuation method's overview, strategic (macro and micro) and financial group analysis on the main markets. Consolidated financial plan was created as a ...

  3. Accuros XB or AAA? Dosimetry study on a heterogeneous medium

    International Nuclear Information System (INIS)

    Accuros XB 1 is a new algorithm introduced by Varian Medical Systems in your Eclipse Planner for external radiotherapy. It is based on the solution of the linear Boltzmann transport equation2. It is necessary to carry out different tests in order to determine its accuracy. This paper examines the variation in absolute doses in presence of inhomogeneities and the results are compared with the results obtained for the Accuros XB and AAA algorithms. (Author)

  4. “Agriculturización” y cambios en el paisaje. El banano en el Atlántico/Caribe de Costa Rica (1870-1930)

    OpenAIRE

    Andrea Montero Mora; Ronny Viales Hurtado

    2014-01-01

    En Costa Rica el cultivo comercial del banano inició en la década de 1870 en el Atlántico/Caribe costarricense. Su impulso debe comprenderse en el contexto del desarrollo del export led growth, que consolidó un estilo de capitalismo agrario basado en la agriculturización/ganaderización. El presente artículo pretende acercarse a la huella visible que dejó la actividad bananera durante su primer ciclo, pues la plantación a gran escala provocó cambios en el paisaje. Comenzaremos con una sucinta ...

  5. Efecto de microorganismos aplicados por fertiriego en la disponibilidad de fósforo en dos sistemas de cultivo de banano en la zona bananera del magdalena.

    OpenAIRE

    Pérez Molina, Sara Mercedes

    2009-01-01

    El trabajo tuvo como objetivo evaluar la disponibilidad de fósforo en el suelo y en el tejido de la planta, los cambios poblacionales de microorganismos eficientes y la correlación de éstos con el pH y las diferentes fracciones de fósforo en el suelo bajo los sistemas de manejo orgánico y convencional de banano en la zona bananera del Magdalena. Se utilizó un diseño completamente al azar con cuatro tratamientos y tres repeticiones; los tratamientos fueron constituidos por tres dosis del produ...

  6. EFECTO DE LA APLICACIÓN DE LOS FUNGICIDAS PROPINEB Y MANCOZEB SOBRE EL ESTADO NUTRICIONAL DE PLÁNTULAS DE BANANO EN MEDIO HIDROPÓNICO

    OpenAIRE

    Juan Carlos M\\u00E9ndez; Floria Bertsch; Oscar Castro

    2013-01-01

    Se evaluó el efecto de la aplicación reiterada y/o alterna de los fungicidas propineb y mancozeb sobre el estado nutricional y el crecimiento de plántulas de banano procedentes de cultivo in vitro. Para ello, se realizó un experimento en cultivo hidropónico con 2 diferentes soluciones nutritivas, con altos y bajos contenidos de Mg y Zn, alto contenido de Mn y niveles óptimos de los demás nutrimentos en ambas soluciones. Los tratamientos, aplicados foliarmente, fueron: testigo, propineb en agu...

  7. Determinación del tiempo de crecimiento para cosecha y comportamiento fisiológico poscosecha del banano variedad Gross Michael

    OpenAIRE

    León Agatón, Libardo; Mejía Gutiérrez, Luis Fernando

    2002-01-01

    La investigación se realizó en la finca “El Manantial”, en la vereda de la Cuchilla del Salado, municipio de Manizales; se escogieron cinco lotes de la finca, cuatro racimos por lote, una gaja por racimo, y de cada gaja se eligieron tres frutos. En el objetivo general se determinó el tiempo de crecimiento del banano variedad “Gross Michael”, con base al desarrollo físico del fruto que establezca el tiempo mínimo de cosecha, y analizar el comportamiento poscosecha a temperaturas de 18° y 28°C....

  8. The well-designed hierarchical structure of Musa basjoo for supercapacitors

    Science.gov (United States)

    Zheng, Kaiwen; Fan, Xiaorong; Mao, Yingzhu; Lin, Jingkai; Dai, Wenxuan; Zhang, Junying; Cheng, Jue

    2016-02-01

    Application of biological structure is one of the hottest topics in the field of science and technology. The unimaginable and excellent architectures of living beings supporting their vital activities have attracted the interests of worldwide researchers. An intriguing example is Musa basjoo which belongs to the herb, while appears like a tree. The profound mystery of structure and potential application of Musa basjoo have not been probed. Here we show the finding of the hierarchical structure of Musa basjoo and the outstanding electrochemical performance of the super-capacitors fabricated through the simple carbonization of Musa basjoo followed by KOH activation. Musa basjoo has three layers of structure: nanometer-level, micrometer-level and millimeter-level. The nanometer-level structure constructs the micrometer-level structure, while the micrometer-level structure constructs the millimeter-level structure. Based on this hierarchical structure, Musa basjoo reduces the unnecessary weight and therefore supports its huge body. The super-capacitors derived from Musa basjoo display a high specific capacitance and a good cycling stability. This enlightening work opens a window for the applications of the natural structure and we hope that more and more people could pay attention to the bio-inspired materials.

  9. Genomics of banana and plantain (Musa spp.), major staple crops in the tropics

    International Nuclear Information System (INIS)

    This chapter on Musa (banana and plantain) genomics covers the latest information on activities and resources developed by the Global Musa Genomics Consortium. Section 4.1 describes the morphology of the plant, its socio-economical importance and usefulness as an experimental organism. Section 4.2 describes the complexity of Musa taxonomy and the importance of genetic diversity Section 4.3 details the genetic maps which have recently been developed and those that are currently being developed. Section 4.4 presents the five BAC libraries which are now publicly available from the Musa Genome Resource Centre and can be distributed in various forms under a material transfer agreement. Section 4.5 gives an overview of cytogenetics and genome organization, showing that the genus Musa has a quite high proportion of repetitive DNA; the discovery of the first pararetrovirus integrated in the genome makes it unique. Section 4.6 explains the first attempts to sequence the genome by BAC end sequencing, whole BAC sequencing, and reduced representation sequencing. Section 4.7 validation using gene trapping, mutation induction and tilling techniques, as well as genetic transformation. Section 4.8 draws overall conclusions. This chapter demonstrated that by organizing the Global Musa Genomics Consortium (currently comprising 33 member institutions from 23 countries), duplication of effort can be minimized and the results of Musa genomics research are rapidly made accessible to taxonomists, breeders and the biotechnology community. (author)

  10. Genomes, diversity and resistance gene analogues in Musa species.

    Science.gov (United States)

    Azhar, M; Heslop-Harrison, J S

    2008-01-01

    Resistance genes (R genes) in plants are abundant and may represent more than 1% of all the genes. Their diversity is critical to the recognition and response to attack from diverse pathogens. Like many other crops, banana and plantain face attacks from potentially devastating fungal and bacterial diseases, increased by a combination of worldwide spread of pathogens, exploitation of a small number of varieties, new pathogen mutations, and the lack of effective, benign and cheap chemical control. The challenge for plant breeders is to identify and exploit genetic resistances to diseases, which is particularly difficult in banana and plantain where the valuable cultivars are sterile, parthenocarpic and mostly triploid so conventional genetic analysis and breeding is impossible. In this paper, we review the nature of R genes and the key motifs, particularly in the Nucleotide Binding Sites (NBS), Leucine Rich Repeat (LRR) gene class. We present data about identity, nature and evolutionary diversity of the NBS domains of Musa R genes in diploid wild species with the Musa acuminata (A), M. balbisiana (B), M. schizocarpa (S), M. textilis (T), M. velutina and M. ornata genomes, and from various cultivated hybrid and triploid accessions, using PCR primers to isolate the domains from genomic DNA. Of 135 new sequences, 75% of the sequenced clones had uninterrupted open reading frames (ORFs), and phylogenetic UPGMA tree construction showed four clusters, one from Musa ornata, one largely from the B and T genomes, one from A and M. velutina, and the largest with A, B, T and S genomes. Only genes of the coiled-coil (non-TIR) class were found, typical of the grasses and presumably monocotyledons. The analysis of R genes in cultivated banana and plantain, and their wild relatives, has implications for identification and selection of resistance genes within the genus which may be useful for plant selection and breeding and also for defining relationships and genome evolution

  11. The complete chloroplast genome of banana (Musa acuminata, Zingiberales: insight into plastid monocotyledon evolution.

    Directory of Open Access Journals (Sweden)

    Guillaume Martin

    Full Text Available BACKGROUND: Banana (genus Musa is a crop of major economic importance worldwide. It is a monocotyledonous member of the Zingiberales, a sister group of the widely studied Poales. Most cultivated bananas are natural Musa inter-(sub-specific triploid hybrids. A Musa acuminata reference nuclear genome sequence was recently produced based on sequencing of genomic DNA enriched in nucleus. METHODOLOGY/PRINCIPAL FINDINGS: The Musa acuminata chloroplast genome was assembled with chloroplast reads extracted from whole-genome-shotgun sequence data. The Musa chloroplast genome is a circular molecule of 169,972 bp with a quadripartite structure containing two single copy regions, a Large Single Copy region (LSC, 88,338 bp and a Small Single Copy region (SSC, 10,768 bp separated by Inverted Repeat regions (IRs, 35,433 bp. Two forms of the chloroplast genome relative to the orientation of SSC versus LSC were found. The Musa chloroplast genome shows an extreme IR expansion at the IR/SSC boundary relative to the most common structures found in angiosperms. This expansion consists of the integration of three additional complete genes (rps15, ndhH and ycf1 and part of the ndhA gene. No such expansion has been observed in monocots so far. Simple Sequence Repeats were identified in the Musa chloroplast genome and a new set of Musa chloroplastic markers was designed. CONCLUSION: The complete sequence of M. acuminata ssp malaccensis chloroplast we reported here is the first one for the Zingiberales order. As such it provides new insight in the evolution of the chloroplast of monocotyledons. In particular, it reinforces that IR/SSC expansion has occurred independently several times within monocotyledons. The discovery of new polymorphic markers within Musa chloroplast opens new perspectives to better understand the origin of cultivated triploid bananas.

  12. Positive Cooperativity of the p97 AAA ATPase Is Critical for Essential Functions*

    OpenAIRE

    Nishikori, Shingo; Esaki, Masatoshi; Yamanaka, Kunitoshi; Sugimoto, Shinya; Ogura, Teru

    2011-01-01

    p97 is composed of two conserved AAA (ATPases associated with diverse cellular activities) domains, which form a tandem hexameric ring. We characterized the ATP hydrolysis mechanism of CDC-48.1, a p97 homolog of Caenorhabditis elegans. The ATPase activity of the N-terminal AAA domain was very low at physiological temperature, whereas the C-terminal AAA domain showed high ATPase activity in a coordinated fashion with positive cooperativity. The cooperativity and coordination are generated by d...

  13. Generation of Five New Musa Hybrids With Resistance To Black Sigatoka and High Yield

    Directory of Open Access Journals (Sweden)

    Ricardo Hernandez

    2007-01-01

    Full Text Available The ability to identify genetic variation is indispensable to effective management and use of genetic resources. This work is the first approach concerning to generation and genetic differentiation of new Musa hybrid lines obtained in INIVIT (Cuba, crossing Musa acuminata male diploid (AA x female triploid (ABB, genotypes selected by their resistant character to pests and diseases. From the obtained hybrids, only five were chosen because of their agronomic behavior and were genetically discriminated among them and also respect to the commercial clone FHIA-18 by AFLP polymorphism. Thanks to this prior genetic characterization Musa breeding programs could be consistently dinamised.

  14. Aortocaval fistula (ACF) in patients operated for ruptured aortic aneurysm (rAAA)

    DEFF Research Database (Denmark)

    Warning, Karina; Houlind, Kim Christian; Ravn, Hans

    Aortocaval fistula (ACF) in patients operated for Ruptured Acute Aorta Aneurysm (rAAA): A surgical challenge. Introduction Aortocaval fistula is a rare complication of abdominal aortic aneurysm (AAA). It been reported to be present in less than 1% of all AAAs. ACF arise in patients with large...... aneurysms and is typically discovered peroperatively in patients with rAAA. Open surgical treatment is associated with high mortality and morbidity. ACF is a result of spontaneously rupture of large atherosclerotic aneurysms into the inferior vena cava in 80%, 15% arise after trauma and 5% are iatrogenic...

  15. Training Scientists to be Effective Communicators: AAAS Communicating Science Workshops

    Science.gov (United States)

    Cendes, L.; Lohwater, T.

    2012-12-01

    "Communicating Science: Tools for Scientists and Engineers" is a workshop program developed by AAAS to provide guidance and practice for scientists and engineers in communicating about science with public audiences. The program was launched at the 2008 AAAS Annual Meeting in Boston and has since provided 24 workshops for more than 1,500 scientist and engineer attendees at universities, science society meetings, and government agency labs around the United States. Each interactive workshop targets scientists and engineers specifically and has included content such as message development, defining audience, identifying opportunities for engaging the public, and practice with public presentations and cameras. The workshop format allows for collaborative learning through small-group discussion, resource sharing, and participation in critique of other participants' presentations. Continuous monitoring of the program includes on-site and online surveys and evaluation. On an assessment of workshops from 2008-2010, attendees reported that knowledge gained from the workshop helped in crafting messages about their scientific work for use in communicating with public audiences, and approximately 80 percent of respondents reported participation in communication with a public audience after attending the workshop. Through workshop content and feedback of participating scientists, this presentation will highlight some best practices and resources for scientists who want to take a proactive role in science communication.

  16. Whole genome sequencing of a banana wild relative Musa itinerans provides insights into lineage-specific diversification of the Musa genus.

    Science.gov (United States)

    Wu, Wei; Yang, Yu-Lan; He, Wei-Ming; Rouard, Mathieu; Li, Wei-Ming; Xu, Meng; Roux, Nicolas; Ge, Xue-Jun

    2016-01-01

    Crop wild relatives are valuable resources for future genetic improvement. Here, we report the de novo genome assembly of Musa itinerans, a disease-resistant wild banana relative in subtropical China. The assembled genome size was 462.1 Mb, covering 75.2% of the genome (615.2Mb) and containing 32, 456 predicted protein-coding genes. Since the approximate divergence around 5.8 million years ago, the genomes of Musa itinerans and Musa acuminata have shown conserved collinearity. Gene family expansions and contractions enrichment analysis revealed that some pathways were associated with phenotypic or physiological innovations. These include a transition from wood to herbaceous in the ancestral Musaceae, intensification of cold and drought tolerances, and reduced diseases resistance genes for subtropical marginally distributed Musa species. Prevalent purifying selection and transposed duplications were found to facilitate the diversification of NBS-encoding gene families for two Musa species. The population genome history analysis of M. itinerans revealed that the fluctuated population sizes were caused by the Pleistocene climate oscillations, and that the formation of Qiongzhou Strait might facilitate the population downsizing on the isolated Hainan Island about 10.3 Kya. The qualified assembly of the M. itinerans genome provides deep insights into the lineage-specific diversification and also valuable resources for future banana breeding. PMID:27531320

  17. PHARMACOLOGICAL SCREENING OF MUSA PARADISICA LINN AGAINST ETHYLENE GLYCOL INDUCED RENAL CALCULI

    Directory of Open Access Journals (Sweden)

    Jha U

    2011-03-01

    Full Text Available The effect of ethanol extract of dried roots of Musa paradisica Linn against ethylene glycol induced renal calculi in albino wistar rats are studied in this research. A renal calculus was induced in rats by ingesting 0.75% ethylene glycol in drinking water for 28 days and was manifested by high urinary calcium, oxalate, and low urinary magnesium contents. Simultaneous administration of 1ml (1 in 10 Musa paradisica Linn orally for 28 days along with ethylene glycol (0.75% v/v reduced urinary calcium, oxalate and elevated urinary magnesium level. It also increased urinary volume thereby reducing the tendency for crystallization. The histopathological studies confirmed the induction as degenerated glomeruli, necrotic tubule and inflammatory cells was observed in section of kidney from animals treated with ethylene glycol. This was reduced; however after treatment with Musa paradisica Linn. These observations enable to conclude that Musa paradisica Linn is effective against ethylene glycol induced renal calculi.

  18. Ascertaining maternal and paternal lineage within Musa by chloroplast and mitochondrial DNA RFLP analyses.

    Science.gov (United States)

    Carreel, F; Gonzalez de Leon, D; Lagoda, P; Lanaud, C; Jenny, C; Horry, J P; Tezenas du Montcel, H

    2002-08-01

    In banana, the maternal transmission of chloroplast DNA and paternal transmission of the mitochondrial DNA provides an exceptional opportunity for studying the maternal and paternal lineage of clones. In the present study, RFLP combined with hybridization of heterologous mitochondrial and chloroplastic probes have been used to characterize 71 wild accessions and 131 diploid and 103 triploid cultivated clones. In additon to Musa acuminata and Musa balbisiana, other species from the four Musa sections were studied to investigate their contribution to the origin of cultivated bananas. These molecular analyses enable the classification of the Musa complex to be discussed. Results ascertain relationships among and between the wild accessions and the mono- and interspecific diploid and triploid bananas, particularly for the acuminata genome. Parthenocarpic varieties are shown to be linked to M. acuminata banksii and M. acuminata errans, thus suggesting that the first center of domestication was in the Philippines - New Guinea area. PMID:12175071

  19. RFLP-based phylogeny of Musa species in Papua New Guinea.

    Science.gov (United States)

    Jarret, R L; Gawel, N; Whittemore, A; Sharrock, S

    1992-08-01

    Random genomic probes were used to detect restriction fragment length polymorphisms (RFLPs) in 26 accessions of Musa representing eight species from Papua New Guinea (PNG), M. textilis, M. jackeyi and one accession of Ensete. Ninety-eight phylogenetically informative characters were scored and analyzed cladistically and phenetically. Results generally agreed with previous morphology-based phylogenetic analyses. However, the closest wild relative of the edible M. fehi (fe'i banana) appears to be M. lolodensis. Musa angustigemma is sister species with M. boman and M. jackeyi and is distinct from M. peekelii, with which it is often united. Musa boman is unambiguously placed in section Australimusa. The diploid parthenocarpic landraces of section Musa unique to PNG are closely related to, but apparently distinct from, M. acuminata ssp. banksii. The evolution of the fe'i bananas and the M. acuminata-derived diploid landraces of PNG are discussed. PMID:24201344

  20. Suppression of Type-II Diabetes with Dyslipidemia and Nephropathy by Peels of Musa cavendish Fruit.

    Science.gov (United States)

    Navghare, Vijay; Dhawale, Shashikant

    2016-10-01

    Musa cavendish, peels has local and traditional use to promote wound healing, hyperglycemia, ulceration etc. The present work investigated the lipid lowering; nephroprotective and glucose lowering properties of ethanolic extract of peels of Musa cavendish (EMC) in alloxan-induced diabetic rats. The EMC 250, 500 and 1000 mg/kg/day and the vehicle were administered orally to alloxan-induced diabetic rats (n = 6) for 3 weeks. Changes in plasma glucose, lipid profile along with kidney function before and after treatment with EMC were recorded. The ethanolic extract of peels of Musa cavendish reduced blood glucose, serum triglyceride, cholesterol, LDL cholesterol and creatinine levels and improvement in body weight, liver glycogen, serum HDL cholesterol, serum albumin and total protein level when compared with untreated rats. Musa cavendish has lipid lowering, nephroprotective and antidiabetic property by regulating glucose uptake in the liver and muscles by restoring the intracellular energy balance. PMID:27605735

  1. Ecophysiological and agronomic response of Abaca (Musa textilis) to different resource conditions in Leyte Island, Philippines

    OpenAIRE

    Bande, Marlito M.

    2012-01-01

    Abaca (Musa textilis Née) is closely related to edible bananas (Musa acuminata Colla and M. balbisiana Colla). Abaca usually thrives in the shade beneath tall trees, especially important for protecting the young plants from the sun and the older, taller plants from wind breakage. However, there is still disagreement on the need for shade trees in abaca cultivation. Hence, this study was conducted to ascertain the ecophysiological and agronomic response of abaca grown in different shade condit...

  2. Phenotypic and molecular characterization of Colletotrichum species associated with anthracnose of banana (Musa spp) in Malaysia.

    Science.gov (United States)

    Intan Sakinah, M A; Suzianti, I V; Latiffah, Z

    2014-01-01

    Anthracnose caused by Colletotrichum species is a common postharvest disease of banana fruit. We investigated and identified Colletotrichum species associated with anthracnose in several local banana cultivars based on morphological characteristics and sequencing of ITS regions and of the β-tubulin gene. Thirty-eight Colletotrichum isolates were encountered in anthracnose lesions of five local banana cultivars, 'berangan', 'mas', 'awak', 'rastali', and 'nangka'. Based on morphological characteristics, 32 isolates were identified as Colletotrichum gloeosporioides and 6 isolates as C. musae. C. gloeosporioides isolates were divided into two morphotypes, with differences in colony color, shape of the conidia and growth rate. Based on ITS regions and β-tubulin sequences, 35 of the isolates were identified as C. gloeosporioides and only 3 isolates as C. musae; the percentage of similarity from BLAST ranged from 95-100% for ITS regions and 97-100% for β-tubulin. C. gloeosporioides isolates were more prevalent compared to C. musae. This is the first record of C. gloeosporioides associated with banana anthracnose in Malaysia. In a phylogenetic analysis of the combined dataset of ITS regions and β-tubulin using a maximum likelihood method, C. gloeosporioides and C. musae isolates were clearly separated into two groups. We concluded that C. gloeosporioides and C. musae isolates are associated with anthracnose in the local banana cultivars and that C. gloeosporioides is more prevalent than C. musae. PMID:24854442

  3. Enhanced yield of phenolic extracts from banana peels (Musa acuminata Colla AAA) and cinnamon barks (Cinnamomum varum) and their antioxidative potentials in fish oil

    OpenAIRE

    Anal, Anil Kumar; Jaisanti, Sirorat; Athapol NOOMHORM

    2012-01-01

    The bioactive compounds of banana peels and cinnamon barks were extracted by vacuum microwave and ultrasonic-assisted extraction methods at pre-determined temperatures and times. These methods enhance the yield extracts in shorter time. The highest yields of both extracts were obtained from the conditions which employed the highest temperature and the longest time. The extracts’ yield from cinnamon bark method was higher by ultrasonic than vacuum microwave method, while vacuum microwave metho...

  4. The role of topolins in micropropagation and somaclonal variation of banana cultivars ´Williams´ and ´Grand Naine´ (Musa spp. AAA)

    Czech Academy of Sciences Publication Activity Database

    Bairu, M. W.; Stirk, W.A.; Doležal, Karel; van Staden, J.

    2008-01-01

    Roč. 95, č. 3 (2008), s. 373-379. ISSN 0167-6857 R&D Projects: GA ČR GA522/06/0108 Institutional research plan: CEZ:AV0Z50380511 Keywords : Abnormality index * Banana * Micropropagation Subject RIV: ED - Physiology Impact factor: 1.017, year: 2008

  5. Combination of membrane technologies for purification of L (+) - lactic acid from juice of banana (Musa AAA, variety Cavendish cultivar Gram naine) obtained from an agroindustrial waste

    International Nuclear Information System (INIS)

    The process that has allowed recovery and purification of the L (+)-acid present in the juice fermented waste produced from banana was developed, treated enzymatically, using tangential nanofiltration. The effect of the enzymatic treatment was evaluated on physical chemical parameters of fermented banana juice. The process parameters of centrifugal clarification and microfiltration were characterized on banana juice as activities prior operations to recovery and purification of lactic acid. The temperature and the transmembrane pressure on the permeate flow and the performance of recovery and purification of lactic acid were evaluated by the ultrafiltration and nanofiltration processes. The properties physico-chemical the banana juice fermented and of the liquid filtrate obtained at the stage recovery and purification of lactic acid were compared by ultrafiltration

  6. Estabelecimento e multiplicação in vitro de brotos no processo de micropropagação de cultivares de bananeira (Musa spp. Establishment and in vitro multiplication of banana (Musa spp. cultivars with the use of PVP (Polyvinylpyrrolidone

    Directory of Open Access Journals (Sweden)

    Hérica Santos de Oliveira

    2011-01-01

    Full Text Available A banana (Musa spp. é uma das frutas mais consumidas no mundo, e amplamente cultivada no Brasil, porém doenças como as sigatokas, negra e amarela, vêm reduzindo a sua produção. A disponibilização imediata de novas cultivares resistentes às principais doenças é limitada pela propagação convencional. A micropropagação é uma alternativa para a produção de mudas com qualidade fitossanitária e vegetativa, mas apresenta fatores que dificultam sua aplicação como a contaminação por fungos e bactérias, associada à oxidação dos explantes. O objetivo desse trabalho foi adaptar e/ou otimizar as etapas do processo de micropropagação para diferentes cultivares de bananeira, por meio do controle de oxidação, contaminação, e multiplicação de brotos, sendo utilizadas as cultivares Caipira (AAA, BRS Caprichosa (AAAB, Pacovan Ken (AAAB, Preciosa (AAAB, PV 03-76 (AAAB, Thap Maeo (AAB. No estudo foram utilizados o antibiótico sulfato de estreptomicina e o fungicida Opera® (BASF visando reduzir a contaminação in vitro provocada por bactérias e fungos, além do anti-oxidante PVP (polivinilpirrolidona para controlar a oxidação. Houve redução da contaminação com uso do sulfato de estreptomicina à concentração de 100 mg L-1 e da oxidação com PVP a 4 g L-1. Na fase de multiplicação de brotos, as cultivares apresentaram médias que variaram de 1,90 a 4,75 brotos/explante. A cultivar caipira (AAA destacou-se das demais com a maior taxa de multiplicação de brotos após três subcultivos, média de 41,50 brotos por rizoma.The banana (Musa spp is one of the most consumed fruits in the world and is widely consumed in Brazil, but diseases such as yellow and black sigatoka have been reducing its production. The immediate availability of new cultivars resistant to major diseases is limited by conventional propagation. The micropropagation, is an alternative for the production of seedlings with phytosanitarium and vegetative

  7. Atividade antifúngica de extratos de Momordica charantia L. sobre Colletotrichum musae Antifungal activity of Momordica charantia L. extracts against Colletotrichum musae

    OpenAIRE

    M.I.B. Celoto; M.F.S. Papa; L.V.S. Sacramento; F.J. Celoto

    2011-01-01

    Os objetivos do presente trabalho foram avaliar os efeitos de extratos de Momordica charantia sobre o crescimento micelial e a germinação de conídios de Colletotrichum musae, e a eficiência destes extratos no controle da antracnose, causada por C. musae, em bananas. Extratos aquoso e hidroetanólico, obtidos de folhas e ramos, na concentração de 50% em relação ao volume adicionado, em meio sólido, proporcionaram 71 e 65% de inibição do crescimento micelial, respectivamente, enquanto que em mei...

  8. ESPECIES DE DRYOPHTHORINAE (COLEOPTERA: CURCULIONIDAE ASOCIADAS A PLÁTANO Y BANANO (Musaspp. EN COLOMBIA Dryophthorinae Species (Coleoptera: Curculionidae Associted to Platain and Banana crops (Musaspp. in Colombia

    Directory of Open Access Journals (Sweden)

    PAULA A SEPÚLVEDA-CANO

    Full Text Available Se presenta una sinopsis de los escarabajos de la subfamilia Dryophthorinae (Coleoptera: Curculionidae asociados a cultivos de plátano y banano en Colombia. Adicionalmente se ofrecen claves ilustradas para las especies del país. Se registran seis especies asociadas a dichos cultivos: Cosmopolites sordidus (Germar, 1824, Metamasius hemipterus (Linnaeus, 1758, Metamasius hebetatus (Gyllenhal, 1838, Metamasius submaculatus Champion, 1910, Rhyncophorus palmarum (Linnaeus, 1758 y Polytus mellerborgii (Boheman, 1838.This synopsis is about beetle&#’;s subfamily Dryophthorinae (Coleoptera: Curculionidae associated to plantain and banana crops. Additionally keys illustrated for the species of the country are offered. Six species associated to these cultures are registered: Cosmopolites sordidus (Germar, 1824, Metamasius hemipterus (Linnaeus, 1758, Metamasius hebetatus (Gyllenhal, 1838, Metamasius submaculatus Champion, 1910, Rhyncophorus palmarum (Linnaeus, 1758 y Polytus mellerborgii (Boheman, 1838.

  9. HOSPEDANTES DE Ralstonia solanacearum EN PLANTACIONES DE BANANO Y PLÁTANO EN COLOMBIA HOSTS OF Ralstonia solanacearum ON BANANA AND PLANTAIN PLANTATIONS IN COLOMBIA

    Directory of Open Access Journals (Sweden)

    Mónica Obregón Barrios

    2008-12-01

    Full Text Available La enfermedad del “Moko” causada por la bacteria Ralstonia solanacearum Raza 2, es uno de los problemas fitopatológicos más limitantes en la producción de plátano y banano en las regiones productoras. La bacteria cuenta con un amplio rango de hospederos, cerca de 50 familias botánicas y más de 200 especies. En plantaciones de banano y plátano de Colombia la bacteria se asocia con arvenses que se encuentran en altas poblaciones contribuyendo a su sobrevivencia y permanencia en el campo. Para la determinación de los hospedantes silvestres sintomáticos y asintomáticos en áreas afectadas en plantaciones de plátano y banano del Valle del Cauca, Quindío y Urabá, se realizó la colección de arvenses frecuentes en los focos afectados y el aislamiento en medio semiselectivo SMSA de la bacteria. 124 aislamientos fueron sometidos a pruebas bioquímicas y de patogenicidad en plantas de banano y plátano bajo condiciones similares a las de Urabá y Valle del Cauca. En las pruebas de patogenicidad 67 de 124 aislamientos evaluados fueron positivos, reproduciendo los síntomas típicos de la enfermedad como flacidez, clorosis, amarillamiento de hojas y muerte de las plantas. Se encontraron nuevas especies de arvenses hospedantes de la bacteria que corresponden a Chaptalia nutans, Seneciodes cinerea, Tripogandra glandulosa, Plenax hirtus, Peperomia pellucida, Tripogandra cumanenses, Desmodium sp, y Cissus sicyoides esta última perteneciente a la familia Vitaceae, la cual corresponde al registro de una nueva familia como hospedera. Se confirmó la presencia de la bacteria en hospedantes silvestres previamente mencionados.The moko disease caused by the bacterium Ralstonia solanacearum race 2, is one of the most limiting phytosanitary problem to deal with in plantain and banana production in Colombia . The bacterium has a wide host range, nearly 50 botanical families and over two hundred species. In plantain and banana plantations in Colombia

  10. Abortifacient and antioxidant activities of different extracts of Musa rosacea

    Institute of Scientific and Technical Information of China (English)

    M. Srikanth; T. Rajananda Swamy; T. Mallikarjuna Rao; B. Ganga Rao

    2013-01-01

    Objective: To evaluate abortifacient and antioxidant activity of Musa rosacea (M. rosacea).Methods:Abortifacient activity was evaluated in rats, compared with standard drug (Mifepristone) and antioxidant activity was evaluated by using three free radicals (Superoxide, Hydroxyl and DPPH). Results: The extracts showed preimplantation loss, postimplantation loss of implantations and decreased the survival ration of foetuses. Among all extracts hydroalcoholic extract showed better activity. The selected plant extracts showed concentration dependent percentage inhibition of free radicals. Among four extracts hydroalcoholic extract showed better activity with IC50 values on superoxide, hydroxyl and DPPH radicals were 180 µg, 218 µg and 116 µg. Conclusion:From the results obtained during the study it could be concluded that M. rosacea extracts have abortifacient and antioxidant components and the results support its folklore usage as abortifacient plant. Further is necessary for isolation and characterization of bioactive molecules which are responsible for these activities.

  11. Improvement of bananas (Musa cvs.) through in vitro anther culture

    International Nuclear Information System (INIS)

    Agricultural products play a great role in the Colombian economy, and the banana is one of the most important. Since 1981, one of the more serious problems effecting production of this crop is the fungus Mycosphaerella fijensis sp. difformis, that causes black sigatoka disease. Most of the recent efforts to control this disease have been directed towards the identification of clones tolerant or resistant to this disease. One alternative approach is the use of anther culture to obtain resistant haploid plants. Diploid clones (Musa - AA) have been used as a model in this study. The results presented here identify the most appropriate stage of anther development for callus induction and proliferation, and treatments that reduce tissue browning. (author). 12 refs, 1 fig., 1 tab

  12. Antidiarrhoeal Activity of Musa paradisiaca Sap in Wistar Rats

    Directory of Open Access Journals (Sweden)

    Musa T. Yakubu

    2015-01-01

    Full Text Available The folkloric claim of Musa paradisiaca sap in the management of diarrhoea is yet to be substantiated or refuted with scientific data. Therefore, the aim of the current study was to screen the sap of M. paradisiaca for both its secondary metabolites and antidiarrhoeal activity at 0.25, 0.50, and 1.00 mL in rats. Secondary metabolites were screened using standard methods while the antidiarrhoeal activity was done by adopting the castor oil-induced diarrhoeal, castor oil-induced enteropooling, and gastrointestinal motility models. The sap contained flavonoids, phenolics, saponins, alkaloids, tannins, and steroids while cardiac glycosides, anthraquinones, triterpenes, cardenolides, and dienolides were not detected. In the castor oil-induced diarrhoeal model, the sap significantly (P<0.05 prolonged the onset time of diarrhoea, decreased the number, fresh weight, and water content of feaces, and increased the inhibition of defecations. Na+-K+-ATPase activity in the small intestine increased significantly whereas nitric oxide content decreased. The decreases in the masses and volumes of intestinal fluid by the sap were accompanied by increase in inhibition of intestinal fluid content in the enteropooling model. The sap decreased the charcoal meal transit in the gastrointestinal motility model. In all the models, the 1.00 mL of the sap produced changes that compared well with the reference drugs. Overall, the antidiarrhoeal activity of Musa paradisiaca sap attributed to the presence of alkaloids, phenolics, flavonoids, and/or saponins which may involve, among others, enhancing fluid and electrolyte absorption through de novo synthesis of the sodium potassium ATPase and/or reduced nitric oxide levels.

  13. Evaluación de métodos estadísticos para el desarrollo de una propuesta de manejo por sitio específico para banano

    OpenAIRE

    Castañeda Sánchez, Darío Antonio

    2011-01-01

    El objetivo general de este trabajo consistió en determinar diferentes propiedades físico-químicas del suelo y el contenido de raíz funcional de las plantas para seleccionar de entre ellas, mediante diferentes métodos, aquellas más relacionadas con la producción de banano en Urabá, con miras a establecer con ellas un plan de manejo por "Sitio específico" dentro de un"Programa de Agricultura de Precisión para Banano" en la región. El trabajo comprende cuatro capítulos. En el capítulo uno se pr...

  14. Effects of gamma irradiation followed by climatization on the quality of 'Prata' banana (Musa acuminata x Musa balbisiana, AAB)

    International Nuclear Information System (INIS)

    Banana is a highly perishable climacteric fruit. In order to ensure fast and homogeneous ripening that will make its processing and commercialization easier, it is usually treated by climatization. On the other hand, irradiation is an interesting physical process capable of delaying ripening and extending the shelf life of fruits. This work investigated the competing effects of irradiation followed by climatization on the quality of 'Prata' banana (Musa acuminata x Musa balbisiana, AAB). All fruits were harvested in the preclimacteric stage, rinsed and stored at 27 deg C after being submitted to five different treatments. After hygienization, four out of five samples were exposed to gamma-ray at doses of 0.25 and 0.50 kGy. The unirradiated fruits were left for control and half of the irradiated samples were then climatized by exposure to CaC2. Measurements of fresh mass loss, total soluble solids, peel coloration and disease index were performed on five different dates for up to nine days in order to monitor quality and the degree of ripening. It was found that high temperatures prevailing during climatization and storage accelerated maturation in all fruits in spite of the use of irradiation, with the control becoming ripe in 3 days only. In addition, based on the consistent results from the experiments performed, it can be concluded that irradiation at the doses tested was unable to overcome the maturation effects produced by climatization. However, the sole use of irradiation with 0.25 kGy yielded the lowest figures for fresh mass loss and disease index in the samples, thus becoming the most attractive among the treatments tested. (author)

  15. Mutations of the AAAS gene in an Indian family with Allgrove's syndrome

    OpenAIRE

    Mukhopadhya, Ashis; Danda, Sumita; Huebner, Angela; Chacko, Ashok

    2006-01-01

    The triple A or Allgrove's syndrome is an autosomal recessive disorder characterized by the triad of achalasia cardia, alacrima and ACTH resistant adrenocortical insufficiency. Mutations of the Achalasia-Addisonianism-Alacrima-Syndrome (AAAS) gene on chromosome 12q13 are associated with this syndrome. We report an Indian family where two siblings were homozygous for a known mutation of the AAAS gene and presented with the classical triad of symptoms. The mother and the brother were heterozygo...

  16. Subunit asymmetry and roles of conformational switching in the hexameric AAA+ ring of ClpX

    OpenAIRE

    Stinson, Benjamin M.; Baytshtok, Vladimir; Schmitz, Karl R.; Baker, Tania A.; Sauer, Robert T.

    2015-01-01

    The hexameric AAA+ ring of Escherichia. coli ClpX, an ATP-dependent protein unfolding and translocation machine, functions with the ClpP peptidase to degrade target substrates. For efficient function, ClpX subunits must switch between nucleotide-loadable (L) and nucleotide-unloadable (U) conformations, but the roles of switching are uncertain. Moreover, it is controversial whether working AAA+ ring enzymes assume symmetric or asymmetric conformations. Here, we show that a covalent ClpX ring w...

  17. On the Impact of Intraluminal Thrombus Mechanical Behavior in AAA Passive Mechanics.

    Science.gov (United States)

    Riveros, Fabián; Martufi, Giampaolo; Gasser, T Christian; Rodriguez-Matas, Jose F

    2015-09-01

    Intraluminal thrombus (ILT) is a pseudo-tissue that develops from coagulated blood, and is found in most abdominal aortic aneurysms (AAAs) of clinically relevant size. A number of studies have suggested that ILT mechanical characteristics may be related to AAA risk of rupture, even though there is still great controversy in this regard. ILT is isotropic and inhomogeneous and may appear as a soft (single-layered) or stiff (multilayered fibrotic) tissue. This paper aims to investigate how ILT constitution and topology influence the magnitude and location of peak wall stress (PWS). In total 21 patient-specific AAAs (diameter 4.2-5.4 cm) were reconstructed from computer tomography images and biomechanically analyzed using state-of-the-art modeling assumptions. Results indicated that PWS correlated stronger with ILT volume (ρ = 0.44, p = 0.05) and minimum thickness of ILT layer (ρ = 0.73, p = 0.001) than with maximum AAA diameter (ρ = 0.05, p = 0.82). On average PWS was 20% (SD 12%) higher for FE models that used soft instead of stiff ILT models (p soft and stiff thrombus tissue, can considerably influence stress in the AAA wall. The present study is limited to identification of influential biomechanical factors, and how its findings translate to an AAA rupture risk assessment remains to be explored by clinical studies. PMID:25636600

  18. Hepatoprotective activity of Musa paradisiaca on experimental animal models

    Institute of Scientific and Technical Information of China (English)

    Nirmala M; Girija K; Lakshman K; Divya T

    2012-01-01

    Objective: To investigate the hepatoprotective activity of stem of Musa paradisiaca (M. paradisiaca) in CCl4 and paracetamol induced hepatotoxicity models in rats. Methods:Hepatoprotective activity of alcoholic and aqueous extracts of stem of M. paradisiaca was demonstrated by using two experimentally induced hepatotoxicity models. Results:Administration of hepatotoxins (CCl4 and paracetamol) showed significant biochemical and histological deteriorations in the liver of experimental animals. Pretreatment with alcoholic extract (500 mg/kg), more significantly and to a lesser extent the alcoholic extract (250 mg/kg) and aqueous extract (500 mg/kg), reduced the elevated levels of the serum enzymes like serum glutamic-oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP) and bilirubin levels and alcoholic and aqueous extracts reversed the hepatic damage towards the normal, which further evidenced the hepatoprotective activity of stem of M.paradisiaca. Conclusions: The alcoholic extract at doses of 250 and 500 mg/kg, p.o. and aqueous extract at a dose of 500 mg/kg, p.o. of stem of M. paradisiaca have significant effect on the liver of CCl4 and paracetamol induced hepatotoxicity animal models.

  19. Valorization and Miscellaneous Prospects of Waste Musa balbisiana Colla Pseudostem

    Directory of Open Access Journals (Sweden)

    Krishna Gogoi

    2014-01-01

    Full Text Available Resourceful utilization of the enormous quantum of agrowastes generated via agricultural practices can be supportive in waste management, environmental upgradation, and subsequent material and energy recovery. In this regard, the present study aimed at highlighting waste banana (Musa balbisiana Colla pseudostem (an agrowaste as a potential bio-based feedstock with miscellaneous applications. The pseudostem was characterized by carbon, nitrogen, and hydrogen (CHN analysis, thermogravimetric-differential thermal analysis (TGDTA, and Fourier transform infrared (FTIR spectroscopy. Cellulose, hemicellulose, and lignin were estimated as a part of biochemical characterization. Total phenolic content, total flavonoid content, 1, 1-diphenyl-2-picrylhydrazyl (DPPH radical scavenging assay, and ferric reducing antioxidant power (FRAP were carried out as a part of antioxidant characterization. The waste banana pseudostem biomass (WBPB was also tried successfully as a natural filler in polyvinyl chloride (PVC polymer composite. Thermal properties and water uptake test of the WBPB polymer composite were accessed as a part of composite characterization. The pseudostem had calorific value (15.22 MJ/kg, high holocellulose (58.67%, high free radical scavenging potential (69.9%, and a low ash content (6.8%. Additionally, the WBPB polymer composite showed improved water resistance and thermostability. The study suggests feasibility of WBPB as a prospective bioenergy feedstock, primary antioxidant source, and reinforcing agent in polymer composites.

  20. Antimicrobial and antihyperglycemic activities of Musa paradisiaca flowers

    Institute of Scientific and Technical Information of China (English)

    Sunil Jawla; Y Kumar; MSY Khan

    2012-01-01

    Objective: To screen the antimicrobial and antihyperglycemic activities of Musa paradisiaca (M. paradisiaca) flowers. Methods: The EtOH and EtOH: water (1:1) extracts of M. paradisiaca flowers were screened for antibacterial and antifungal activity against standard strains of Bacillussubtilis (K. pneumoniae), Proteus mirabilis (P. mirabilis), Pseudomonas aeruginosa (P. aeruginosa),Streptococcus pneumoniae (B. subtilis), Bacillus cereus (B. cereus), Escherichia coli (E. coli), Klebsiella pneumoniae typhimurium (S. typhimurium) and Candida albicans (C. albicans), Cryptococcus albidus (C.albidus (S. pneumoniae), Staphylococcus aureus (S. aureus), Salmonella ) against amikacin and clotrimazole respectively. Both the extracts were also administered to normal and alloxan induced diabetic rats. The blood glucose levels were measured daily after oral administration of extracts at doses of 100, 250 and 500 mg/(kg.d). Result: The EtOH and EtOH:water (1:1) extracts exhibited antimicrobial activity with minimum inhibitory concentrations ranging from 5.62-25.81 and 7.60-31.50 μg/mL respectively. Both the extracts reversed the permanent hyperglycemia within a week in alloxan induced diabetic rats. The EtOH extract (250 mg/kg) was found to be 7.69% more potent hypoglycemic effect than standard oral hypoglycemic drug, glibenclamide 0.2 mg/kg b.w., respectively. Conclusion: The alcoholic extracts of M. paradisiaca flowers showed potent antihyperglycemic and moderate antimicrobial activities.

  1. Surface Coating of Musa Brachycarpa Trunk Using UV-Radiation

    International Nuclear Information System (INIS)

    An experiment on UV-curing of surface coating of Musa brachycarpa was carried out using urethane acrylate polymer films. Radiation curable material was the mixture of urethane acrylate resin, tripropylene glycol diacrylate monomer (TPGDA) and radical photo initiator of 2,2-dimethyl -2-hydroxy acetophenone. The TPGDA concentrations in the mixture with urethane acrylate resin were 60; 70 and 80% weight, Whereas concentrations of photo initiator were varied at the level 1.5:2.0 and 2.5% by weight based on resin and monomer mixture. Irradiation was conducted by using 80 Watt/cm intensity UV-light at the conveyor speed of 2: 3 and 4 m/min. Analysis and film properties observed were IR spectrum, gel fraction, hardness, abrasion resistance, glossy and chemical, solvent and stain resistances. Films have good resistances against 1% sodium carbonate, 5% acetic acid, 50% alcohol, thinner and red, blue and black permanent marker, except against 10% sodium hydroxide and 10% sulfuric acid. Optimum condition was achieved at the photo initiator concentration level of 2% and conveyor speed of 3 m/min

  2. Atividade antifúngica de extratos de Momordica charantia L. sobre Colletotrichum musae Antifungal activity of Momordica charantia L. extracts against Colletotrichum musae

    Directory of Open Access Journals (Sweden)

    M.I.B. Celoto

    2011-01-01

    Full Text Available Os objetivos do presente trabalho foram avaliar os efeitos de extratos de Momordica charantia sobre o crescimento micelial e a germinação de conídios de Colletotrichum musae, e a eficiência destes extratos no controle da antracnose, causada por C. musae, em bananas. Extratos aquoso e hidroetanólico, obtidos de folhas e ramos, na concentração de 50% em relação ao volume adicionado, em meio sólido, proporcionaram 71 e 65% de inibição do crescimento micelial, respectivamente, enquanto que em meio líquido, a inibição do crescimento micelial foi de 86 e 81%, respectivamente. Somente o extrato aquoso e o tiofanato metílico, nas concentrações de 50% e 1000 µg mL-1 respectivamente, proporcionaram 100% de inibição da germinação de esporos de C. musae. Os extratos metanólico e aquoso inibiram em 80 e 70%, respectivamente, o desenvolvimento das lesões em bananas, quando aplicados até dois dias antes da inoculação do fungo. Estes resultados foram semelhantes ao tratamento com tiofanato metílico, que inibiu 80% do desenvolvimento das lesões. Confirma-se a presença de substância antifúngica nos extratos de M. charantia e outros estudos devem ser realizados para viabilizar seu uso no controle da antracnose da banana.The aims of the present work were to evaluate the effects of Momordica charantia extracts on mycelial growth and conidial germination of Colletotrichum musae, as well as the efficiency of these extracts in controlling anthracnose caused by C. musae in bananas. Water and hydroethanol extracts were obtained from leaves and branches at 50% concentration relative to the added volume. In solid medium, extracts led to 71 and 65% mycelial growth inhibition, respectively, whereas in liquid medium the mycelial growth was inhibited at 86 and 81%, respectively. Only water extract and thiophanate-methyl, at 50% and 1000 µg mL-1, respectively, resulted in 100% inhibition of C. musae spore germination. Methanol and water

  3. AAA application in diagnosis exams in a large public hospital, RS, Brazil; Aplicacao do AAA na realizacao de exames diagnosticos em um hospital publico de grande porte

    Energy Technology Data Exchange (ETDEWEB)

    Bacelar, A.; Ferret, A.A.; Vanni, S.; Galhardi, M.P.; Lykawka, R., E-mail: abacelar@hepa.ufrgs.br, E-mail: allferret@gmail.com, E-mail: svanni@hepa.ufrgs.br, E-mail: mpgalhardi@gmail.com, E-mail: rlykawka@hepa.ufrgs.br [Hospital de Clinicas de Porto Alegre (HCPA), Porto Alegre, RS (Brazil)

    2013-10-01

    Objective: the initiative AAA - Awareness , Appropriateness and Audit , promotes consciousness ( Awareness) , fitness ( Appropriateness ) and Audit ( Audit) . This paper analyzes the application of the concept in the AAA requests and justifications examinations using ionizing radiation within a large public hospital. Materials and methods: we collected and analyzed data between the years 2011 and 2012, concerning the number of exams performed with the use of radiation and their justifications. After, we sought to raise awareness of the clinical team through training on the risks and benefits of the various modalities of the radiology department and the need to justify the use of ionizing radiation on health. After the data were collected again of test requests for verification of the effectiveness of training. Results: the mean requests that need to be appropriate to the AAA in the last quarter of 2011 was 75 % lower than the average demands of the first quarter, matched against the last two months of 2012 increased by up to four times the number requests that require improvements in relation to the excellent results obtained in July 2012. Conclusion: it is shown in this paper the need of implementing this initiative AAA continuously added to the clinical staff awareness about the risk of the use of ionizing radiation, the appropriateness of the requests of these tests , as well as the control of this process in order to optimize use of ionizing radiation on health.

  4. Obtención de Etanol y Biogás a Partir de Banano de Rechazo Biogas and Bioethanol Production from Non-Exportable Low Quality Banana

    Directory of Open Access Journals (Sweden)

    Carlos A Guevara

    2012-01-01

    Full Text Available Se desarrolló un proceso fermentativo mediante hidrólisis endógena inducida para producir etanol a partir de banano verde no apto para exportación. El potencial de las vinazas también fue evaluado para obtener biogás. A escala de 1 litro se comparó la hidrólisis endógena inducida con la hidrólisis exógena usando enzimas comerciales, y la hidrólisis ácida tomando como punto de referencia el etanol producido en la fermentación. Con la hidrólisis endógena se obtuvo los mayores rendimientos y con esta metodología se realizaron fermentaciones a 15 litros Los rendimientos de etanol en promedio fueron 0.065 litros por kilogramo de banano verde y los del biogás fueron 2.24 litros por litro de vinaza. El proceso propuesto presenta rendimientos comparables con los de la fermentación de caña de azúcar. Además presenta ventajas, tales como su bajo costo, fácil operación y por ser una alternativa de solución ambientalmente compatible en el área del manejo de residuos de cosecha.In this study was developed a fermentative process to produce ethanol from green banana non optimal for exportation through one induced endogenous hydrolysis. The potential of the stillage to produce biogas was also evaluated. It was compared the induced endogenous hydrolysis, with the exogenous hydrolysis that uses commercial enzymes and the traditional acid hydrolysis done all of them in a final volume of 1 liter, the reference point was the ethanol produced in the fermentation process. The maximum performance was obtained with the endogenous hydrolysis and with these methodology fermentations at 15 liter as done. The average of ethanol produced was 0.065 liters from one kilogram of green banana and the biogas production was 2.24 liter per liter of stillage. The proposed process shows performance comparable with that obtained by sugar cane fermentation. Also the methodology demonstrated several advantages, such as low cost, simple to operate, and because it

  5. DETERMINATION OF POTASH ALKALI AND METAL CONTENTS OF ASHES OBTAINED FROM PEELS OF SOME VARIETIES OF NIGERIA GROWN MUSA SPECIES

    Directory of Open Access Journals (Sweden)

    Joshua Olajiire Babayemi

    2010-05-01

    Full Text Available Potash alkali and metal contents of ashes obtained from peels of six varieties of Nigeria Musa species were investigated. The varieties of Musa species – Musa paradisiaca (plantain, Musa ‘Gross Michel’ (Igbo banana, M.sapientum L. (paranta, Musa ‘Wild Banana’ (omini, Musa ‘Red’ (sweet banana, and Musa ‘Fugamo’ (somupeke, were investigated. The moisture, dry matter, ash and alkali contents; concentration of metals in the ashes and in the contents extracted with water from the ashes; and the ratio of potassium to other metals in the ashes and in the corresponding extracts were determined. Moisture contents ranged from 80.9 to 86.7%; dry matter content, 13.3 to 19.1%; ash content, 6.3 to 12.0%; alkali content, 69.0 to 81.9% of ash and 4.7 to 9.6% of dry sample. Samples ranged between 2.60 and 720mg/kg and in the corresponding extracts, BDL to 500.49mg/kg; ratio of concentration of potassium to other metals in the samples, 0.6 to 395; and in the extracts, 0.5 to 313. Gross michel showed the highest concentration of K (750mg/kg while omini banana gave the lowest average value (112.70mg/kg.

  6. EFEITO DO PESO DE MUDA TIPO PEDAÇO DE RIZOMA NO ENRAIZAMENTO INICIAL DA BANANEIRA DO CULTIVAR NANICÃO (Musa acuminata AAA EFFECT OF THE WEIGHT OF RHIZOME TYPE SEEDLING IN THE INITIAL ROOTING OF BANANA CULTIVAR NANICÃO (Musa acuminata AAA

    Directory of Open Access Journals (Sweden)

    Lincoln F. Zica

    2007-09-01

    Full Text Available

    O uso de mudas do tipo pedaço de rizoma, vem, a cada dia, sendo procurado intensivamente como material de propagação de bananeiras. Porém, ao se trabalhar com esse tipo de mudas há necessidades de se conhecer o efeito do peso do rizoma em relação ao seu enraizamento inicial, pois quanto maior o peso das raízes, melhores serão as mudas. De acordo com a análise estatística concluiu-se que há um maior enraizamento em pedaços de rizomas de maior peso.

    Pieces of rootstalk are being used more and more intensively as a means of reproducing banana plants. However, in order to work effectively with this means of reproduction, it is necessary to know the effect of the rootstalk’s weight when it is first planted, because the greater the weight of the rootstalk, the better the shoots will be. On the basis of statistical analysis, it was concluded that the heavier pieces of rootstalk root better.

  7. CORRELATIONS BETWEEN COMMERCIALIZATION UNITS OF BANANA "NANICÃO" (Musa acuminata AAA IN GOIÁS, BRAZIL CORRELAÇÕES ENTRE UNIDADES DE COMERCIALIZAÇÃO PARA BANANAS “NANICÃO” (Musa acuminata AAA EM GOIÁS

    Directory of Open Access Journals (Sweden)

    Ronaldo Veloso Naves

    2007-09-01

    Full Text Available

    In Goiás, wholesalers of the “Giant Cavendish” banana use different systems of measuring. This causes constant confusion. This study was undertaken to try to establish empirical formulas which can be used to correlate the diverse systems.

    Na comercialização de bananas do sub-grupo cavendishi, em Goiás, utiliza-se de várias unidades de medida. Esta diversidade causa problemas no bom entendimento entre vendedores e compradores. Com a finalidade de estabelecer fórmulas empíricas de transformações de unidades de comercialização, empreendeu-se o presente trabalho. Com base nos resultados, conclui-se que as melhores fórmulas de transformações são as seguintes: a - Peso de um carregamento em cachos para número de frutas (NF = 80,4 P/ 11,41, sendo P tomado em Kg; b - Para a obtenção do peso líquido ( PL de frutas a serem encaixadas, subtrai-se 10% do peso bruto; c - Peso líquido (PL de frutas em caixas tipo torito (Cx (Cx = PL/ 18,85; d - Cento de frutas (CF em caixas tipo torito (Cx (Cx = CF/ 14.

  8. Structural and Function Prediction of Musa acuminata subsp. Malaccensis Protein

    Directory of Open Access Journals (Sweden)

    Anum Munir

    2016-03-01

    Full Text Available Hypothetical proteins (HPs are the proteins whose presence has been anticipated, yet in vivo function has not been built up. Illustrating the structural and functional privileged insights of these HPs might likewise prompt a superior comprehension of the protein-protein associations or networks in diverse types of life. Bananas (Musa acuminata spp., including sweet and cooking types, are giant perennial monocotyledonous herbs of the order Zingiberales, a sister grouped to the all-around considered Poales, which incorporate oats. Bananas are crucial for nourishment security in numerous tropical and subtropical nations and the most prominent organic product in industrialized nations. In the present study, the hypothetical protein of M. acuminata (Banana was chosen for analysis and modeling by distinctive bioinformatics apparatuses and databases. As indicated by primary and secondary structure analysis, XP_009393594.1 is a stable hydrophobic protein containing a noteworthy extent of α-helices; Homology modeling was done utilizing SWISS-MODEL server where the templates identity with XP_009393594.1 protein was less which demonstrated novelty of our protein. Ab initio strategy was conducted to produce its 3D structure. A few evaluations of quality assessment and validation parameters determined the generated protein model as stable with genuinely great quality. Functional analysis was completed by ProtFun 2.2, and KEGG (KAAS, recommended that the hypothetical protein is a transcription factor with cytoplasmic domain as zinc finger. The protein was observed to be vital for translation process, involved in metabolism, signaling and cellular processes, genetic information processing and Zinc ion binding. It is suggested that further test approval would help to anticipate the structures and functions of other uncharacterized proteins of different plants and living being.

  9. Control of Anthracnose Caused by Colletotrichum musae on Curcuma alismatifolia Gagnep. Using Antagonistic Bacillus spp.

    Directory of Open Access Journals (Sweden)

    Supuk Mahadtanapuk

    2007-01-01

    Full Text Available Over 400 bacterial strains, isolated from leaf surfaces of Curcuma alismatifolia Gagnep. and hot springs in the Chiang Mai province of northern Thailand, were screened in vitro for antagonistic activity against Colletotrichum musae, an anthracnose fungus. Three isolates provided greater than 75% growth inhibition of the fungus in vitro and were identified as Bacillus licheniformis, B. amyloliquefaciens and B. subtilis. Using in planta tests, B. amyloliquefaciens and B. subtilis were shown to efficiently colonize the curcuma bracts, provide a statistically significant growth suppression of C. musae over that of B. licheniformis, and all three isolates could provide 100% inhibition of conidial fungal germination. When B. licheniformis was co-inoculated in combination with either of the other two bacteria, the ability of B. amyloliquefaciens and B. subtilis to suppress the fungal disease was dramatically reduced. Both B. amyloliquefaciens and B. subtilis were found to contain an isoform of iturin A with antifungal activity against C. musae. As a preventative measure to control the spread of C. musae and reduce the severity of fungal infections, B. amyloliquefaciens could be used to inoculate curcuma flowers cost effectively and reduce the need for the toxic synthetic fungicides currently in use.

  10. Assessment of Ploidy and Genome Constitution of Some Musa balbisiana Cultivars using DArT Markers

    Czech Academy of Sciences Publication Activity Database

    Sales, E. K.; Butardo, N. G.; Paniagua, H. G.; Jansen, H.; Doležel, Jaroslav

    2011-01-01

    Roč. 36, č. 1 (2011), s. 11-18. ISSN 0115-463X Institutional research plan: CEZ:AV0Z50380511 Keywords : DArT * genome * Musa balbisiana Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 0.075, year: 2011 http://home.ueb.cas.cz/publikace/2011_Sales_PHILIPPINE_JOURNAL_OF_CROP_SCIENCE_11.pdf

  11. Catalog of banana (Musa spp.) accessions maintained at the USDA-ARS, Tropical Agriculture Reserach Station

    Science.gov (United States)

    Banana genetic resources can be found in situ in native habitats in Southeast Asia and the Pacific region. Ex situ collections also exist in important tropical regions of the world as well as in vitro cultures at the Bioversity International Musa Germplasm Transit Centre. Unfortunately, readily avai...

  12. Consumer Perceptions towards Introducing a Genetically Modified Banana (Musa spp.) in Uganda

    NARCIS (Netherlands)

    Kikulwe, E.M.; Wesseler, J.H.H.; Falck-Zepeda, J.

    2010-01-01

    The introduction of a genetically modified (GM) banana (Musa spp.) in Uganda is not without controversy. It is likely to generate a wide portfolio of concerns as the technology of genetic engineering is still in its early stages of development in Uganda. The purpose of this study is to show how cons

  13. Evaluation and characterization in bananas (Musa ssp.) at the USDA-ARS Tropical Agriculture Research Station

    Science.gov (United States)

    Banana, Musa spp., is a key horticultural crop in tropical regions of the world where they provide sustenance and serve as cash crops. The plantain subgroup in particular, is an important staple in the Caribbean, Central America and some countries in South America. One of the integral research comp...

  14. Genetic resources in Musa bananas and improvement of their disease resistance

    International Nuclear Information System (INIS)

    The cultivated bananas belong to the genus Musa and it is the wild species Musa acuminata and Musa balbisiana which contributed to the origin of the sorts used as food. Most of these are triploids and possess a high degree of sterility. The sources of variation that are of importance for genetic improvement of the bananas are (1) hereditary differences between the cultivated clones; (2) difference between species and sub-species; (3) differences between the primitive cultivars derived from Musa acuminata, and (4) mutations that can be artiificially induced. The bananas are attacked by many diseases. Their vulnerability to certain diseases is highly significant in view of the extreme genetic uniformity of the commercial crops and the absence of resistant genes. In the past the wild species and the diploids used as food served as sources of resistance. However, efforts to induce resistance in the cultivated triploid bananas have not been successful. The use of mutagenic agents is proposed as a possible way of improving genetic variability in banana cultivation. (author)

  15. Usefulness of embryogenic cell suspension for the induction and selection of mutants in Musa spp

    Czech Academy of Sciences Publication Activity Database

    Roux, N.; Toloza, A.; Doležel, Jaroslav; Swennen, R.; Lepoivre, P.; Zapata-Arias, F. J.

    2002. s. 17-18. [ FAO /IAEA Research Co-ordination Meeting on Cellular Biology and Biotechnology Including Mutation Techniques for Creation of New Useful Banana Genotypes /4./. 24.09.2002-28.09.2002, Leuven] Institutional research plan: CEZ:AV0Z5038910 Keywords : cell suspension * mutagenesis * Musa spp. Subject RIV: EB - Genetics ; Molecular Biology

  16. “Agriculturización” y cambios en el paisaje. El banano en el Atlántico/Caribe de Costa Rica (1870-1930

    Directory of Open Access Journals (Sweden)

    Andrea Montero Mora

    2014-03-01

    Full Text Available En Costa Rica el cultivo comercial del banano inició en la década de 1870 en el Atlántico/Caribe costarricense. Su impulso debe comprenderse en el contexto del desarrollo del export led growth, que consolidó un estilo de capitalismo agrario basado en la agriculturización/ganaderización. El presente artículo pretende acercarse a la huella visible que dejó la actividad bananera durante su primer ciclo, pues la plantación a gran escala provocó cambios en el paisaje. Comenzaremos con una sucinta explicación del auge bananero en la región, para después analizar los nuevos paisajes y las nuevas formas de vida que surgieron con la introducción de la musácea y culminar describiendo el paisaje diverso y humanizado de las plantaciones bananeras, lo anterior en el marco liberal decimonónico de orden y progreso.

  17. Indigenous anti-ulcer activity of Musa sapientum on peptic ulcer

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    P Prabha

    2011-01-01

    Full Text Available Background: Peptic ulcer disease (PUD, encompassing gastric and duodenal ulcers is the most prevalent gastrointestinal disorder. The pathophysiology of PUD involves an imbalance between offensive factors like acid, pepsin and defensive factors like nitric oxide and growth factors. The clinical evaluation of antiulcer drugs showed tolerance, incidence of relapses and side-effects that make their efficacy arguable. An indigenous drug like Musa sapientum possessing fewer side-effects is the major thrust area of present day research, aiming at a better and safer approach for the management of PUD. Material and Methods: The unripe plantain bananas (Musa sapientum were shade-dried, powdered and used for phytochemical analysis and as antiulcer drug. In our present study Group I rats served as control and were treated with saline, Group II was indomethacin-induced ulcerated rats, Group III received aqueous extract of Musa sapientum along with indomethacin and Group IV received esomeprazole along with indomethacin for 21 days. The anti-ulcerogenic activity was investigated by performing hematological, mucosal, antioxidant profile in comparison with the standard drug esomeprazole. Results: Our findings from High - Performance Thin Layer Chromatography (HPTLC analysis showed that Musa sapientum has an active compound a monomeric flavonoid (leucocyanidin with anti-ulcerogenic activity. Results were expressed as mean ± SD. All our results are in congruous with the results of standard drug esomeprazole. Conclusion: It could be clearly concluded that administration of the aqueous extract of Musa sapientum at the dose used in this study tends to ameliorate ulcers. Its use in indigenous medicine should be scientifically scrutinized with further research.

  18. Musa's granite and Rio Maria's granodiorite Rb/Sr isotopic ages and geochemistry

    International Nuclear Information System (INIS)

    The Musa Granite and the Rio Maria Granodiorite are located at the eastern margin of the Amazonian craton, in the Rio Maria region, where a typical granite-greenstone terrain is characterized. Rb-Sr dating of six samples from different facies of Rio Maria Granodiorite furnished an age of 2564 ± 68 Ma with initial 87Sr/86Sr radio (IR) of 0.70288 ± 0.00092 (whole rock isochron; 1 ο error; MSWD = 2.26). Thirteen samples from the three facies of Musa Granite (monzogranites, syenogranites and intermediate to felsic hypabyssal rocks) gave Rb-Sr whole rock isochron with an age of 1692 ± 11 Ma and IR of 0.70777 ± 0.00023 (1 ο error, MSWD = 1.89). A preliminary attempt to individualize geochronologically the three facies was done resulting different ages and IRs. There is a coincidence between these ages and the emplacement sequence of these facies of the pluton. The actual meaning of the Rio Maria Granodiorite Rb-Sr age is still uncertain. It could be related to the end of the magmatic crystallization of the batholith as well as to the metamorphic-mylonitic event that affect it. Considering that the Jamon and Musa Granites are petrologically similar that they occur in the same area, it is interesting to note that latter is apparently a little older than the former. The IRs obtained for the two plutons are also not coincident. The isotopic Rb-Sr available data show that the exposed rocks of the Rio Maria Granodiorite have not been able to generate magmas with the compositions of the monzongranitic and the hypabyssal facies of the Musa pluton. On the other hand, rocks isotopically similar to the Rio Maria Granodiorite would theorically be able to generate the Jamon and a magma with the characteristics of the syenogranitic facies of the Musa pluton. (author)

  19. Improvement of the banana "Musa acuminata" reference sequence using NGS data and semi-automated bioinformatics methods

    Czech Academy of Sciences Publication Activity Database

    Martin, G.; Baurens, F.C.; Droc, G.; Rouard, M.; Cenci, A.; Kilian, A.; Hastie, A.; Doležel, Jaroslav; Aury, J. M.; Alberti, A.; Carreel, F.; D'Hont, A.

    2016-01-01

    Roč. 17, MAR 16 (2016), s. 243. ISSN 1471-2164 Institutional support: RVO:61389030 Keywords : Musa acuminata * Genome assembly * Bioinformatics tool Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.986, year: 2014

  20. Investigation of Solvent Effects on NMR Shielding Tensors of Tripeptide Tyr-Aaa-Gly

    Directory of Open Access Journals (Sweden)

    Ebrahim Balali

    2014-09-01

    Full Text Available The unique structure of adamantane is reflected in its highly unusual physical and chemical properties, which can have many applications including drug design and drug delivery. Tripeptide Tyr-Aaa-Gly is methionine-enkephalin analogues [Aaa = (R,S-(1-adamantylglycine]. In this research, according to Quantum-mechanical calculations nuclear, shielding parameters of Tripeptide Tyr-Aaa-Gly, such as chemical shift isotropic value (σiso and the anisotropy shielding (σaniso , ∆σ, and other parameter such as δ, η and atomic charge water have been taken into account using GIAO method at the HF method with the 3-21G, 6-31G and 6-31G* basis sets and at the B3LYP method with the 3-21G, 6-31G and STO-3G basis sets in the gas phase and in different solvents such as, DMSO, methanol, ethanol and dichloromethane.

  1. Efecto fungistático de extractos y aceites esenciales de LippiaoriganoidesHBK y ThymusvulgarisL. como alternativas de manejo de Colletotrichummusae en banano y Botrytiscinerea en fresa

    OpenAIRE

    Taborda Andrade, Luís Alejandro

    2013-01-01

    La antracnosis del banano (Colletotrichummusae) y la cenicilla de la fresa (Botrytiscinerea) son dos enfermedades que producen importantes pérdidas poscosecha. En el presente estudio se evaluó el efecto fungistático de extractos y aceites esenciales de Lippiaoriganoides y Thymusvulgaris (a concentraciones de 500, 256 y 128 mg/l) sobre ColletotrichummusaeyBotrytiscinerea in vitroe in vivo. En el ensayo in vitro se determinó el porcentaje de inhibición del crecimiento micelial. Se observó que l...

  2. Análisis de secuencias expresadas diferencialmente de la variedad “Calcutta 4” (Musa AA) en respuesta a mycosphaerella fijiensis morelet mediante microarreglos / Analysis of differentially expressed sequences from banana cultivar “Calcutta 4” (Musa AA) in response to mycosphaerella fijiensi morelet by microarrays

    OpenAIRE

    Rodríguez Cabal, Hector Alejandro

    2011-01-01

    Los cultivos de banano y plátano son importantes para muchos países como producto de alimentación básica y para exportación. La enfermedad causada por el hongo Mycosphaerella fijiensis es uno de los problemas fitosanitarios que más perdidas ocasiona en cultivos de banano y plátano. Son muchas las alternativas que se han implementado para el control de la enfermedad, algunas con mejor resultado que otras. Sin embargo, actualmente se depende casi exclusivamente de productos químicos para su man...

  3. Phyllosticta musarum Infection-Induced Defences Suppress Anthracnose Disease Caused by Colletotrichum musae in Banana Fruits cv ‘Embul’

    OpenAIRE

    Abayasekara, C. L.; Adikaram, N. K. B.; Wanigasekara, U. W. N. P.; B.M.R. Bandara

    2013-01-01

    Anthracnose development by Colletotrichum musae was observed to be significantly less in the fruits of the banana cultivar ‘Embul’ (Mysore, AAB) infected with Phyllosticta musarum than in fruits without such infections. Anthracnose disease originates from quiescent C. musae infections in the immature fruit. P. musarum incites minute, scattered spots, referred to as freckles, in the superficial tissues of immature banana peel which do not expand during maturation or ripening. P. musarum does n...

  4. Analysis of the leaf transcriptome of Musa acuminata during interaction with Mycosphaerella musicola: gene assembly, annotation and marker development

    OpenAIRE

    Passos, Marco A. N.; de Cruz, Viviane Oliveira; Emediato, Flavia L; de Teixeira, Cristiane Camargo; Azevedo, Vânia C Rennó; Brasileiro, Ana C. M.; Amorim, Edson P; Ferreira, Claudia F; Martins, Natalia F; Togawa, Roberto C; Pappas, Georgios J; da Silva, Orzenil Bonfim; Miller, Robert NG

    2013-01-01

    Background Although banana (Musa sp.) is an important edible crop, contributing towards poverty alleviation and food security, limited transcriptome datasets are available for use in accelerated molecular-based breeding in this genus. 454 GS-FLX Titanium technology was employed to determine the sequence of gene transcripts in genotypes of Musa acuminata ssp. burmannicoides Calcutta 4 and M. acuminata subgroup Cavendish cv. Grande Naine, contrasting in resistance to the fungal pathogen Mycosph...

  5. Communication between the AAA+ ring and microtubule-binding domain of dynein1

    OpenAIRE

    Carter, Andrew P.; Vale, Ronald D.

    2010-01-01

    Dyneins are microtubule motors, the core of which consists of a ring of AAA+ domains. ATP-driven conformational changes of the AAA+ ring are used to drive the movement of a mechanical element (termed the linker domain) that provides the motor’s powerstroke and to change the affinity of the motor for microtubules (strong binding during the power stroke and weak binding to allow stepping and recocking of the linker domain). Dynein’s microtubule-binding domain (MTBD) is located at the end of a 1...

  6. Dynamic and static components power unfolding in topologically closed rings of a AAA+ proteolytic machine

    OpenAIRE

    Glynn, Steven E.; Nager, Andrew R.; Baker, Tania A.; Sauer, Robert T.

    2012-01-01

    In the E. coli ClpXP protease, a hexameric ClpX ring couples ATP binding and hydrolysis to mechanical protein unfolding and translocation into the ClpP degradation chamber. Rigid-body packing between the small AAA+ domain of each ClpX subunit and the large AAA+ domain of its neighbor stabilizes the hexamer. By connecting the parts of each rigid-body unit with disulfide bonds or linkers, we created covalently closed rings that retained robust activity. A single-residue insertion in the hinge t...

  7. Pharmacological evaluation of Musa seminifera Lour.fruit

    Institute of Scientific and Technical Information of China (English)

    Sanjib Saha; Faroque Hossain; Md.Anisuzzman; Md.Khirul Islam

    2013-01-01

    OBJECTIVE:To study potential antioxidant,analgesic,antidiarrheal,and antibacterial activities of the ethanol extract of Musa seminifera Lour.fruit in different established in vivo and in vitro experimental models.METHODS:In vitro antioxidant activity was studied in 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay.Phenolic content was determined using Folin-Ciocalteu's reagent.Reducing ability was evaluated by ferric reducing power assay.Peripherally and centrally acting analgesic activity was studied in three different in vivo models,namely,acetic acid-induced writhing,hot-plate test,and tail-flick test in Swiss albino mice.In vivo antidiarrheal activity was evaluated in castor oil-and magnesium sulfate-induced diarrhea in mice.Gastrointestinal motility test was also carried out in mice.All studies in mice were undertaken at the doses of 250 and 500 mg/kg body weight.Antibacterial activity was assessed by disk diffusion assay against some Gram-positive and Gram-negative bacterial strains.Acute toxicity test was conducted to assess the safe doses of the extract.RESULTS:The extract showed 50% inhibitory concentration value of 12.65 μg/mL in DPPH radicalscavenging assay.Phenolic content was found to be 589.83 mg gallic acid equivalent per 100 g of dried fruits extract.Reducing power was in a concentration-dependent manner,and strongly comparable with the standard ascorbic acid.The extract demonstrated significant inhibition of writhing in acetic acid-induced writhing test at both dose levels (P<0.01).The extract also raised pain threshold in both hot-plate and tail-flick test in a dose-dependent manner,and the results were statistically significant (P<0.01).The extract significantly (P<0.01) increased latent period,and decreased defecation in both castor oil-and magnesium sulfate-induced diarrhea.The extract also decreased gastrointestinal motility in mice.In disk diffusion assay,the extract showed potential antibacterial activity against all the

  8. Dilatation of the proximal neck of infrarenal aortic aneurysms after endovascular AAA repair

    NARCIS (Netherlands)

    Wever, JJ; de Nie, AJ; Blankensteijn, JD; Broeders, IAMJ; Mail, WPTM; Eikelboom, BC

    2000-01-01

    Objectives: to assess size changes of the proximal aortic neck after endograft placement. Methods: since 1994, 54 consecutive patients have undergone abdominal aortic aneurysm (AAA) repair with the Endovascular Technologies (EVT) endograft. The study group comprised the 33 patients who had completed

  9. AAA application in diagnosis exams in a large public hospital, RS, Brazil

    International Nuclear Information System (INIS)

    Objective: the initiative AAA - Awareness , Appropriateness and Audit , promotes consciousness ( Awareness) , fitness ( Appropriateness ) and Audit ( Audit) . This paper analyzes the application of the concept in the AAA requests and justifications examinations using ionizing radiation within a large public hospital. Materials and methods: we collected and analyzed data between the years 2011 and 2012, concerning the number of exams performed with the use of radiation and their justifications. After, we sought to raise awareness of the clinical team through training on the risks and benefits of the various modalities of the radiology department and the need to justify the use of ionizing radiation on health. After the data were collected again of test requests for verification of the effectiveness of training. Results: the mean requests that need to be appropriate to the AAA in the last quarter of 2011 was 75 % lower than the average demands of the first quarter, matched against the last two months of 2012 increased by up to four times the number requests that require improvements in relation to the excellent results obtained in July 2012. Conclusion: it is shown in this paper the need of implementing this initiative AAA continuously added to the clinical staff awareness about the risk of the use of ionizing radiation, the appropriateness of the requests of these tests , as well as the control of this process in order to optimize use of ionizing radiation on health

  10. Structure of Lmaj006129AAA, a hypothetical protein from Leishmania major

    International Nuclear Information System (INIS)

    The crystal structure of a conserved hypothetical protein from L. major, Pfam sequence family PF04543, structural genomics target ID Lmaj006129AAA, has been determined at a resolution of 1.6 Å. The gene product of structural genomics target Lmaj006129 from Leishmania major codes for a 164-residue protein of unknown function. When SeMet expression of the full-length gene product failed, several truncation variants were created with the aid of Ginzu, a domain-prediction method. 11 truncations were selected for expression, purification and crystallization based upon secondary-structure elements and disorder. The structure of one of these variants, Lmaj006129AAH, was solved by multiple-wavelength anomalous diffraction (MAD) using ELVES, an automatic protein crystal structure-determination system. This model was then successfully used as a molecular-replacement probe for the parent full-length target, Lmaj006129AAA. The final structure of Lmaj006129AAA was refined to an R value of 0.185 (Rfree = 0.229) at 1.60 Å resolution. Structure and sequence comparisons based on Lmaj006129AAA suggest that proteins belonging to Pfam sequence families PF04543 and PF01878 may share a common ligand-binding motif

  11. Structure of Lmaj006129AAA, a hypothetical protein from Leishmania major

    Energy Technology Data Exchange (ETDEWEB)

    Arakaki, Tracy; Le Trong, Isolde [Department of Biochemistry, University of Washington, Seattle, WA 98195-7742 (United States); Structural Genomics of Pathogenic Protozoa (SGPP) Consortium (United States); Phizicky, Eric; Quartley, Erin [Structural Genomics of Pathogenic Protozoa (SGPP) Consortium (United States); Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642 (United States); DeTitta, George; Luft, Joseph; Lauricella, Angela [Structural Genomics of Pathogenic Protozoa (SGPP) Consortium (United States); Hauptman-Woodward Institute, Buffalo, NY 14203 (United States); Anderson, Lori; Kalyuzhniy, Oleksandr [Department of Biochemistry, University of Washington, Seattle, WA 98195-7742 (United States); Structural Genomics of Pathogenic Protozoa (SGPP) Consortium (United States); Worthey, Elizabeth; Myler, Peter J. [Structural Genomics of Pathogenic Protozoa (SGPP) Consortium (United States); Seattle Biomedical Research Institute, Seattle, WA 98109 (United States); Kim, David [Structural Genomics of Pathogenic Protozoa (SGPP) Consortium (United States); Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195 (United States); Baker, David; Hol, Wim G. J. [Department of Biochemistry, University of Washington, Seattle, WA 98195-7742 (United States); Structural Genomics of Pathogenic Protozoa (SGPP) Consortium (United States); Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195 (United States); Merritt, Ethan A., E-mail: merritt@u.washington.edu [Department of Biochemistry, University of Washington, Seattle, WA 98195-7742 (United States); Structural Genomics of Pathogenic Protozoa (SGPP) Consortium (United States)

    2006-03-01

    The crystal structure of a conserved hypothetical protein from L. major, Pfam sequence family PF04543, structural genomics target ID Lmaj006129AAA, has been determined at a resolution of 1.6 Å. The gene product of structural genomics target Lmaj006129 from Leishmania major codes for a 164-residue protein of unknown function. When SeMet expression of the full-length gene product failed, several truncation variants were created with the aid of Ginzu, a domain-prediction method. 11 truncations were selected for expression, purification and crystallization based upon secondary-structure elements and disorder. The structure of one of these variants, Lmaj006129AAH, was solved by multiple-wavelength anomalous diffraction (MAD) using ELVES, an automatic protein crystal structure-determination system. This model was then successfully used as a molecular-replacement probe for the parent full-length target, Lmaj006129AAA. The final structure of Lmaj006129AAA was refined to an R value of 0.185 (R{sub free} = 0.229) at 1.60 Å resolution. Structure and sequence comparisons based on Lmaj006129AAA suggest that proteins belonging to Pfam sequence families PF04543 and PF01878 may share a common ligand-binding motif.

  12. Ripening of fruits of 'Dwarf Prata' banana (Musa acuminata x Musa balbisiana, AAB group)irradiated and treated with calcium carbide

    Energy Technology Data Exchange (ETDEWEB)

    Martineli, Maristella [Instituto de Quimica. Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ (Brazil); Coneglian, Regina C.C.; Vasconcellos, Marco A.S.; Silva, Eduardo, E-mail: rccconeg@ufrrj.br, E-mail: masv@ufrrj.br [Departamento de Fitotecnia. Instituto de Agronomia. Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropedica, RJ (Brazil); Vital, Helio C., E-mail: vital@ctex.eb.br [Secao de Defesa Nuclear. Divisao de Defesa Quimica, Biologica e Nuclear. Centro Tecnologico do Exercito (CTEx), Guaratiba, Rio de Janeiro, RJ (Brazil)

    2011-07-01

    The competing effects resulting from the exposure of fruits of 'warf prata' banana (Musa acuminata x Musa balbisiana, AAB group) to gamma radiation and to calcium carbide have been investigated in this work. The fruits were harvested in a pre-climateric stage (green colored though physiologically developed) in the city of Jaiba, state of Minas Gerais, and gamma irradiated with doses of 0.25 or 0.50 kGy in a research irradiating facility at the Brazilian Army Technology Center (CTEx) in the city of Rio de Janeiro. Some samples were also exposed to calcium carbide for 32 hours in order to accelerate ripening. Quantitative estimates of peel color, disease index and fresh mass loss were performed for 9 days while the fruits were kept at an average temperature of 23 deg C. The analyses were performed in the Federal Rural University of Rio de Janeiro, located in the city of Seropedica. The following treatments or combination of processes have been tested: untreated (control); treated only with irradiation with doses of 0.25 kGy or 0.50 kGy; treated with irradiation with doses of 0.25 kGy or 0.50 kGy and then exposed to calcium carbide. The fruits treated solely with irradiation with 0.25 kGy exhibited a better response during the first days of storage, although their initial green coloration vanished with time. In addition, the fungi Colletotrichum musae and Lasidioplodia theobroma were detected in samples submitted to the combination of both processes. In contrast, such fungi were not observed in fruits that had only been exposed to 0.25 kGy and exhibited low disease indices. Also, 1-2 cm lesions were detected on fruits.(author)

  13. Medicinal activities of the leaves of Musa sapientum var. sylvesteris in vitro

    Institute of Scientific and Technical Information of China (English)

    Repon Kumer Sahaa; Srijan Acharyaa; Syed Sohidul Haque; Shovon Priyanka Royb

    2013-01-01

    This study is to investigate the medicinal value of methanolic extract of the leaves of Musa sapientum var. sylvesteris in Bangladesh. Methods: Several biochemical assays, thin layer chormatogarphy and ultra-violet spectroscopy were used to detect the presence of various types of compounds in this extract. Antioxidant effects were measured by DPPH scavenging assay, total reducing assay and hydrogen peroxide scavenging assay. Receptor binding activities and hydrogen peroxide induced hemolysis assay were performed by hemagglutination assay and hemolysis assay using erythrocytes. Disk diffusion assay was performed to show the antibacterial effect of the extract. Results: Methanolic extract of the leaves showed antioxidant and antibacterial activity in vitro. The extract showed hemaglutination inhibition activities and hydrogen peroxide induced hemolysis inhibition activity of human red blood cells. Conclusion:Musa sapientum var. sylvesteris can be an useful medicinal plant.

  14. A saturated SSR/DArT linkage map of Musa acuminata addressing genome rearrangements among bananas

    Directory of Open Access Journals (Sweden)

    Matsumoto Takashi

    2010-04-01

    Full Text Available Abstract Background The genus Musa is a large species complex which includes cultivars at diploid and triploid levels. These sterile and vegetatively propagated cultivars are based on the A genome from Musa acuminata, exclusively for sweet bananas such as Cavendish, or associated with the B genome (Musa balbisiana in cooking bananas such as Plantain varieties. In M. acuminata cultivars, structural heterozygosity is thought to be one of the main causes of sterility, which is essential for obtaining seedless fruits but hampers breeding. Only partial genetic maps are presently available due to chromosomal rearrangements within the parents of the mapping populations. This causes large segregation distortions inducing pseudo-linkages and difficulties in ordering markers in the linkage groups. The present study aims at producing a saturated linkage map of M. acuminata, taking into account hypotheses on the structural heterozygosity of the parents. Results An F1 progeny of 180 individuals was obtained from a cross between two genetically distant accessions of M. acuminata, 'Borneo' and 'Pisang Lilin' (P. Lilin. Based on the gametic recombination of each parent, two parental maps composed of SSR and DArT markers were established. A significant proportion of the markers (21.7% deviated (p Conclusions We propose a synthetic map with 11 linkage groups containing 489 markers (167 SSRs and 322 DArTs covering 1197 cM. This first saturated map is proposed as a "reference Musa map" for further analyses. We also propose two complete parental maps with interpretations of structural rearrangements localized on the linkage groups. The structural heterozygosity in P. Lilin is hypothesized to result from a duplication likely accompanied by an inversion on another chromosome. This paper also illustrates a methodological approach, transferable to other species, to investigate the mapping of structural rearrangements and determine their consequences on marker

  15. Repetitive part of the banana (Musa acuminata) genome investigated by low-depth 454 sequencing

    OpenAIRE

    Hřibová Eva; Neumann Pavel; Matsumoto Takashi; Roux Nicolas; Macas Jiří; Doležel Jaroslav

    2010-01-01

    Abstract Background Bananas and plantains (Musa spp.) are grown in more than a hundred tropical and subtropical countries and provide staple food for hundreds of millions of people. They are seed-sterile crops propagated clonally and this makes them vulnerable to a rapid spread of devastating diseases and at the same time hampers breeding improved cultivars. Although the socio-economic importance of bananas and plantains cannot be overestimated, they remain outside the focus of major research...

  16. Consumer Perceptions towards Introducing a Genetically Modified Banana (Musa spp.) in Uganda

    OpenAIRE

    Kikulwe, E.M.; Wesseler, J.H.H.; Falck-Zepeda, J.

    2010-01-01

    The introduction of a genetically modified (GM) banana (Musa spp.) in Uganda is not without controversy. It is likely to generate a wide portfolio of concerns as the technology of genetic engineering is still in its early stages of development in Uganda. The purpose of this study is to show how consumers feel about GM banana biosafety risks and the potential challenges for marketing the product. The study analyzes socio-demographic characteristics, awareness and attitudes of banana-consuming ...

  17. Effect of gamma irradiation on ripening, storage and biochemical parameters of banana (Musa paradisiaca L.)

    International Nuclear Information System (INIS)

    Banana (Musa paradisiaca L.) popularly known as kadali (S) belongs to the family Musaceae is an economically important plant due to its nutritive fruit used as staple food all over the world. Several techniques have been employed to delay fruit ripening and softening by storing preclimacteric bananas in modified or controlled atmospheres. The Food and Drug Administration (FDA) has recommended the usage up to 1.0 kGy of gamma irradiation for delaying the ripening in fruits. Irradiation significantly improved shelf life by inhibiting the fungal growth in strawberries. Hence, present work was undertaken to scrutinize the impact of gamma irradiation on the ripening, storage and the biochemical attributes of Musa paradisiaca L. variety kadali. Present work revealed that irradiation at 0.2 kGy and 0.3 kGy retained their organoleptic properties till 16th day of storage against under normal conditions. Nutritional parameters such as ascorbic acid content, soluble sugar and protein contents were significantly higher in irradiated fruits. Hence, lower dose of gamma irradiation could be successfully used in extending the shelf life and nutritional quality and delay the process of ripening in Musa paradisiaca L.

  18. Phyllosticta musarum Infection-Induced Defences Suppress Anthracnose Disease Caused by Colletotrichum musae in Banana Fruits cv ‘Embul’

    Directory of Open Access Journals (Sweden)

    C. L. Abayasekara

    2013-03-01

    Full Text Available Anthracnose development by Colletotrichum musae was observed to be significantly less in the fruits of the banana cultivar ‘Embul’ (Mysore, AAB infected with Phyllosticta musarum than in fruits without such infections. Anthracnose disease originates from quiescent C. musae infections in the immature fruit. P. musarum incites minute, scattered spots, referred to as freckles, in the superficial tissues of immature banana peel which do not expand during maturation or ripening. P. musarum does not appear to have a direct suppressive effect on C. musae as conidia of C. musae germinate on both freckled and non-freckled fruit forming quiescent infections. Our investigations have shown that P. musarum infection induced several defence responses in fruit including the accumulation of five phytoalexins, upregulation of chitinase and β-1,3-glucanase, phenylalanine ammonia lyase (PAL activity and cell wall lignification. ¹H and ¹³C NMR spectral data of one purified phytoalexin compared closely with 4′-hydroxyanigorufone. Some of the P. musarum-induced defences that retained during ripening, restrict C. musae development at the ripe stage. This paper examines the potential of P. musarum-induced defences, in the control of anthracnose, the most destructive postharvest disease in banana.

  19. Phyllosticta musarum Infection-Induced Defences Suppress Anthracnose Disease Caused by Colletotrichum musae in Banana Fruits cv 'Embul'.

    Science.gov (United States)

    Abayasekara, C L; Adikaram, N K B; Wanigasekara, U W N P; Bandara, B M R

    2013-03-01

    Anthracnose development by Colletotrichum musae was observed to be significantly less in the fruits of the banana cultivar 'Embul' (Mysore, AAB) infected with Phyllosticta musarum than in fruits without such infections. Anthracnose disease originates from quiescent C. musae infections in the immature fruit. P. musarum incites minute, scattered spots, referred to as freckles, in the superficial tissues of immature banana peel which do not expand during maturation or ripening. P. musarum does not appear to have a direct suppressive effect on C. musae as conidia of C. musae germinate on both freckled and non-freckled fruit forming quiescent infections. Our investigations have shown that P. musarum infection induced several defence responses in fruit including the accumulation of five phytoalexins, upregulation of chitinase and β-1,3-glucanase, phenylalanine ammonia lyase (PAL) activity and cell wall lignification. (1)H and (13)C NMR spectral data of one purified phytoalexin compared closely with 4'-hydroxyanigorufone. Some of the P. musarum-induced defences that retained during ripening, restrict C. musae development at the ripe stage. This paper examines the potential of P. musarum-induced defences, in the control of anthracnose, the most destructive postharvest disease in banana. PMID:25288931

  20. Phyllosticta musarum Infection-Induced Defences Suppress Anthracnose Disease Caused by Colletotrichum musae in Banana Fruits cv ‘Embul’

    Science.gov (United States)

    Abayasekara, C. L.; Adikaram, N. K. B.; Wanigasekara, U. W. N. P.; Bandara, B. M. R.

    2013-01-01

    Anthracnose development by Colletotrichum musae was observed to be significantly less in the fruits of the banana cultivar ‘Embul’ (Mysore, AAB) infected with Phyllosticta musarum than in fruits without such infections. Anthracnose disease originates from quiescent C. musae infections in the immature fruit. P. musarum incites minute, scattered spots, referred to as freckles, in the superficial tissues of immature banana peel which do not expand during maturation or ripening. P. musarum does not appear to have a direct suppressive effect on C. musae as conidia of C. musae germinate on both freckled and non-freckled fruit forming quiescent infections. Our investigations have shown that P. musarum infection induced several defence responses in fruit including the accumulation of five phytoalexins, upregulation of chitinase and β-1,3-glucanase, phenylalanine ammonia lyase (PAL) activity and cell wall lignification. 1H and 13C NMR spectral data of one purified phytoalexin compared closely with 4′-hydroxyanigorufone. Some of the P. musarum-induced defences that retained during ripening, restrict C. musae development at the ripe stage. This paper examines the potential of P. musarum-induced defences, in the control of anthracnose, the most destructive postharvest disease in banana. PMID:25288931

  1. Estructura de mercado en la comercialización del banano tipo exportación producido en la Zona Bananera del Magdalena y comportamiento estratégico de las empresas

    Directory of Open Access Journals (Sweden)

    Janny Acuña Fuentes

    2014-01-01

    Full Text Available La estructura de mercado en la comercialización del banano tipo exportación producido en el departamento del Magdalena históricamente ha sido imperfecta, siendo las comercializadoras multinacionales quienes fijan las cantidades de banano que compran a los productores, o el precio, generando una pérdida de bienestar social a los productores de banano. En la presente investigación se midió el grado de concentración de mercado por parte de las comercializadoras a través del índice de concentración Herfindahl-Hirschman (IHH; posteriormente, a través de econometría de series de tiempo, se procedió a mostrar la relación entre los precios pagados a los productores y el IHH, por último, mediante el modelo de Cournot y de Stackelberg, a partir de la estimación econométrica de una función inversa de oferta y la aplicación de estrategias puras en juegos no cooperativos, se probó empíricamente las estrategias que las comercializadoras están llevando a cabo.Palabras Clave: Cournot; Stackelberg; Bertrand; Equilibrio de Nash; índice Herfindahl-Hirschman; teoría de juegos Market structure in the marketing of bananas export type produced in the area of Magdalena and strategic business behaviorAbstractThe market structure in the marketing of banana export type produced in the department of Magdalena historically has been imperfect, with multinational marketers who will determine the quantities of bananas they buy from the producers or the price, generating a loss of social welfare to banana producers. In the present study measured the degree of market concentration by marketers across the Herfindahl concentration index-Heirschman (HHI, then through econometric time series proceeded to show the relationship between the prices paid to producers and the concentration index (HHI, finally using the model of Cournot and Stackelberg, from the econometric estimation of an inverse function of supply and application of pure strategies in

  2. Dosimetric comparison of Acuros XB, AAA, and XVMC in stereotactic body radiotherapy for lung cancer

    International Nuclear Information System (INIS)

    Purpose: To compare the dosimetric performance of Acuros XB (AXB), anisotropic analytical algorithm (AAA), and x-ray voxel Monte Carlo (XVMC) in heterogeneous phantoms and lung stereotactic body radiotherapy (SBRT) plans. Methods: Water- and lung-equivalent phantoms were combined to evaluate the percentage depth dose and dose profile. The radiation treatment machine Novalis (BrainLab AG, Feldkirchen, Germany) with an x-ray beam energy of 6 MV was used to calculate the doses in the composite phantom at a source-to-surface distance of 100 cm with a gantry angle of 0°. Subsequently, the clinical lung SBRT plans for the 26 consecutive patients were transferred from the iPlan (ver. 4.1; BrainLab AG) to the Eclipse treatment planning systems (ver. 11.0.3; Varian Medical Systems, Palo Alto, CA). The doses were then recalculated with AXB and AAA while maintaining the XVMC-calculated monitor units and beam arrangement. Then the dose-volumetric data obtained using the three different radiation dose calculation algorithms were compared. Results: The results from AXB and XVMC agreed with measurements within ±3.0% for the lung-equivalent phantom with a 6 × 6 cm2 field size, whereas AAA values were higher than measurements in the heterogeneous zone and near the boundary, with the greatest difference being 4.1%. AXB and XVMC agreed well with measurements in terms of the profile shape at the boundary of the heterogeneous zone. For the lung SBRT plans, AXB yielded lower values than XVMC in terms of the maximum doses of ITV and PTV; however, the differences were within ±3.0%. In addition to the dose-volumetric data, the dose distribution analysis showed that AXB yielded dose distribution calculations that were closer to those with XVMC than did AAA. Means ± standard deviation of the computation time was 221.6 ± 53.1 s (range, 124–358 s), 66.1 ± 16.0 s (range, 42–94 s), and 6.7 ± 1.1 s (range, 5–9 s) for XVMC, AXB, and AAA, respectively. Conclusions: In the phantom

  3. Motivation of employees in the organization - case study of AAA auto group

    OpenAIRE

    Vznuzdaeva, Anastasia

    2013-01-01

    This bachelor thesis deals with the matter of motivation in AAA Auto Group N.V. Work considers motivation as one of possibilities to effectively lead people and achieve outcomes. The work consists of two parts. The first part is a theoretical that talks about the management and its administrative functions, describes motivation and stimulation patterns, characterizes different theories of motivation and management strategies for increasing motivation. The second part of the work concentrat...

  4. Indications for and outcome of open AAA repair in the endovascular era.

    Science.gov (United States)

    Wieker, Carola M; Spazier, Max; Böckler, Dittmar

    2016-04-01

    The benefits, safety and efficacy of endovascular aortic aneurysm repair (EVAR) is well documented and intensively reported in multiple randomized trials and meta-analysis. Therefore, EVAR became the first choice of abdominal aortic aneurysms (AAA) treatment in almost 70-100% of patients. Consecutively, open repair (OR) is performed less frequently in morphologically preselected patients. Anatomical condition remains the most important factor for indication for OR. Especially unfavorable intrarenal landing zone based on difficult neck anatomy like very short neck or excessive neck angulation is still the most predictive factor. Furthermore, patients presenting additional iliac aneurysms, aortoiliac occlusive disease or variations of renal arteries are recommended for OR. Randomized trials like EVAR 1, DREAM and OVER from the year 2004/2005 and 2009 showed lower 30-day mortality rates in EVAR compared to OR. However, the late mortality rates after two years became equal in both treatment options. Furthermore, reinterventions after EVAR occur more frequently than after OR. Analysis from our own data showed a higher 30-day mortality in the patients who underwent OR in the endovascular era (15% vs. 2.5%), however the number of emergency open AAA repair because of ruptured aneurysms was much higher in the endovascular era (32.5% vs. 5%). In conclusion, treatment of AAA has changed in the past decade. Nevertheless OR of AAA still remains as a safe and durable method in experienced surgeons, even in the endovascular era. High volume centres are needed to offer the best patients' treatment providing the best postoperative outcome. Therefore OR must remain a part of fellowship training in the future. To decide the best treatment option many facts like patients' fitness and preference or finally the anatomic suitability for endovascular repair have to be considered. PMID:26822580

  5. A Localized Authentication, Authorization, and Accounting (AAA) Protocol for Mobile Hotspots

    OpenAIRE

    Baek, Sungmin; Pack, Sangheon; Kwon, Taekyoung; Choi, Yanghee

    2006-01-01

    Mobile hotspots, i.e. Internet access services in moving networks (e.g. vehicular area networks (VAN) and personal area networks (PAN)) bring about new challenging issues. Even if the network mobility (NEMO) basic support protocol has been standardized as a mobility solution by the Internet Engineering Task Force (IETF), to the best of our knowledge, no studies have been conducted in the area of authentication, authorization, and accounting (AAA) protocol, which is a core technology for publi...

  6. Caracterização fisiomorfológica de isolados de Colletotrichum musae Physiological and morphological characterization of Colletotrichum musae isolates

    Directory of Open Access Journals (Sweden)

    Erick F. Couto

    2004-08-01

    Full Text Available Isolados de Colletotrichum musae obtidos de quatro cultivares de banana (Musa spp. 'Comprida', 'Maçã', 'Pacovan' e 'Prata' foram estudados quanto ao aspecto morfológico dos conídios, apressórios, características culturais, diâmetro das colônias em meio BDA, germinação dos conídios em água destilada esterilizada e meio líquido BD, como também, quanto ao efeito da combinação de C/N no crescimento micelial, esporulação e peso da matéria seca, sob alternância luminosa, a aproximadamente, 25 ºC. No estudo da relação C/N, as fontes de carbono foram dextrose, sacarose e sorbitol e as de nitrogênio asparagina, peptona e nitrato de potássio combinadas na proporção 10:1 (10 g de carbono para 1 g de nitrogênio. Os resultados mostraram conídios hialinos, com forma e tamanhos característicos da espécie, variando dentro dos limites estabelecidos para a espécie. A relação comprimento/largura foi menor para os isolados Iso-1, Iso-6 e Iso-8 oriundos de banana 'Comprida'. A germinação de conídios ocorreu a partir de 8 h de incubação, havendo diferença significativa entre os isolados, quanto ao percentual de conídios germinados. Foram observados apressórios em todos os isolados, variando em quantidade. Houve diversidade nas características culturais e diâmetro das colônias dos isolados, em BDA. Com relação às combinações C/N, a análise estatística revelou diferença significativa entre os isolados, sob efeito da interação C/N, bem como dos fatores independentes, sobre o crescimento micelial, produção de esporos e peso seco do micélio. De um modo geral, as combinações de carbono com peptona favoreceram esses três processos fisiológicos, porém com diferença significativa entre os isolados de C. musae dentro de cada processo considerado.Isolates of Colletotrichum musae from four banana (Musa spp. cultivars 'Comprida', 'Maçã', 'Pacovan' and 'Prata' were studied in relation to morphological aspects of

  7. Functional characterization of fidgetin, an AAA-family protein mutated in fidget mice

    International Nuclear Information System (INIS)

    The mouse fidget mutation is an autosomal recessive mutation that renders reduced or absent semicircular canals, microphthalmia, and various skeletal abnormalities to affected mice. We previously identified the defective gene which encodes fidgetin, a new member of the ATPases associated with diverse cellular activities (AAA proteins). Here, we report on the subcellular localization of fidgetin as well as that of two closely related proteins, fidgetin-like 1 and fidgetin-like 2. Epitope-tagging and immunostaining revealed that both fidgetin and fidgetin-like 2 were predominantly localized to the nucleus, whereas fidgetin-like 1 was both nuclear and cytoplasmic. Furthermore, deletion studies identified a putative bipartite nuclear localization signal in the middle portion of the fidgetin protein. Since AAA proteins are known to form functional hetero- or homo-hexamers, we used reciprocal immunoprecipitation to examine the potential interaction among these proteins. We found that fidgetin interacted with itself and this specific interaction was abolished when either the N- or C-terminus of the protein was truncated. Taken together, our results suggest that fidgetin is a nuclear AAA-family protein with the potential to form homo-oligomers, thus representing the first step towards the elucidation of fidgetin's cellular function and the disease mechanism in fidget mutant mice

  8. Structural Insights into the Allosteric Operation of the Lon AAA+ Protease.

    Science.gov (United States)

    Lin, Chien-Chu; Su, Shih-Chieh; Su, Ming-Yuan; Liang, Pi-Hui; Feng, Chia-Cheng; Wu, Shih-Hsiung; Chang, Chung-I

    2016-05-01

    The Lon AAA+ protease (LonA) is an evolutionarily conserved protease that couples the ATPase cycle into motion to drive substrate translocation and degradation. A hallmark feature shared by AAA+ proteases is the stimulation of ATPase activity by substrates. Here we report the structure of LonA bound to three ADPs, revealing the first AAA+ protease assembly where the six protomers are arranged alternately in nucleotide-free and bound states. Nucleotide binding induces large coordinated movements of conserved pore loops from two pairs of three non-adjacent protomers and shuttling of the proteolytic groove between the ATPase site and a previously unknown Arg paddle. Structural and biochemical evidence supports the roles of the substrate-bound proteolytic groove in allosteric stimulation of ATPase activity and the conserved Arg paddle in driving substrate degradation. Altogether, this work provides a molecular framework for understanding how ATP-dependent chemomechanical movements drive allosteric processes for substrate degradation in a major protein-destruction machine. PMID:27041592

  9. Characterization of Resistance Gene Analogs in Musa acuminata Cultivars Contrasting in Resistance to Biotic Stresses

    International Nuclear Information System (INIS)

    The majority of commercial banana cultivars (Musa sp.) have evolved via asexual vegetative propagation, with diversity dependent upon somatic mutation. Restricted variation has resulted in a crops with little resistance to pests and disease, and conventional breeding efforts are limited due to limited viable seed production. Numerous disease resistance genes (R-genes / R-proteins) have been characterized in plants, recognizing and conferring resistance to bacteria, virus, fungi and nematodes. The identification and cloning of R-genes in Musa would contribute to germplasm improvement. To date, five main R-gene classes have been identified, based upon protein domains, with the most abundant coding for nucleotide-binding site-leucine-rich repeat (NBS-LRR) proteins. Primers designed from conserved protein motifs have enabled amplification of NBS homologues across diverse plant species. In Musa, our group has identified over 50 distinct NBS-LRR type resistance gene analogs (RGAs) in the resistant wild diploid M. acuminata Calcutta 4. The aim of this work was to characterize RGAs in M. acuminata cultivars contrasting in resistance to Black leaf Streak Disease. PCR amplification was conducted using DNA from M. acuminata cultivars Calcutta 4 (resistant) and Pisang Berlin (susceptible). Degenerate primers targeted sequences homologous to the NBS-LRR R-gene family. Following sequencing and processing of cloned PCR products, 63 out of a total of 136 high quality sequences showed homology to R-genes or RGAs. Phylogenetic analysis was conducted on deduced amino-acid sequences. Degenerate primers were also developed targeting an R-gene family of cytoplasmic serine-threonine (Ser/Thr) receptor-like kinases (RLKs) with extracellular LRRs, for application across cultivars. Studies are also planned for selection and full length sequencing of clones from M. acuminata and M. balbisiana BAC libraries containing novel RGAs characterized in this study, as an approach for complete R

  10. Characterization of resistance gene analogs in Musa acuminata cultivars contrasting in resistance to biotic stresses

    International Nuclear Information System (INIS)

    The majority of commercial banana cultivars (Musa sp.) have evolved via asexual vegetative propagation, with diversity dependent upon somatic mutation. Restricted variation has resulted in a crop with little resistance to pests and disease, and conventional breeding efforts are limited due to limited viable seed production. Numerous disease resistance genes (R-genes / R-proteins) have been characterized in plants, recognizing and conferring resistance to bacteria, virus, fungi and nematodes. The identification and cloning of R-genes in Musa would contribute to germplasm improvement. To date, five main R-gene classes have been identified, based upon protein domains, with the most abundant coding for nucleotidebinding site-leucine-rich repeat (NBS-LRR) proteins. Primers designed from conserved protein motifs have enabled amplification of NBS homologues across diverse plant species. In the case of Musa, our group has identified over 50 distinct NBS-LRR type resistance gene analogs (RGAs) in the resistant wild diploid M. acuminata Calcutta 4. The objective of this work was to characterize RGAs in M. acuminata cultivars contrasting in resistance to Black leaf Streak Disease. PCR amplification was conducted using DNA from M. acuminata cultivars Calcutta 4 (resistant) and Pisang Berlin (susceptible). Degenerate primers targeted sequences homologous to the NBS-LRR R-gene family. Following sequencing and processing of cloned PCR products, 63 out of a total of 136 high quality sequences showed homology to R-genes or RGAs. Phylogenetic analysis was conducted on deduced amino-acid sequences. Degenerate primers were also developed targeting an R-gene family of cytoplasmic serine-threonine (Ser/Thr) receptor-like kinases (RLKs) with extracellular LRRs, for application across cultivars. Studies are also planned for selection and full length sequencing of clones from M. acuminata and M. balbisiana BAC libraries containing novel RGAs characterized in this study, as an approach for

  11. Effects of gamma irradiation followed by climatization on the quality of 'Prata' banana (Musa acuminata x Musa balbisiana, AAB)

    Energy Technology Data Exchange (ETDEWEB)

    Martineli, Maristella; Coneglian, Regina C.C.; Vasconcellos, Marco A.S.; Silva, Eduardo; Rocha, Janielio G.; Melo, Maruzanete P. [Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropedica, RJ. Inst. de Agronomia (Brazil)], e-mail: maristellamartineli@yahoo.com.br; Vital, Helio C. [Centro Tecnologico do Exercito (CTEx), Rio de Janeiro, RJ (Brazil). Secao de Defesa Nuclear], e-mail: vital@ctex.eb.br

    2009-07-01

    Banana is a highly perishable climacteric fruit. In order to ensure fast and homogeneous ripening that will make its processing and commercialization easier, it is usually treated by climatization. On the other hand, irradiation is an interesting physical process capable of delaying ripening and extending the shelf life of fruits. This work investigated the competing effects of irradiation followed by climatization on the quality of 'Prata' banana (Musa acuminata x Musa balbisiana, AAB). All fruits were harvested in the preclimacteric stage, rinsed and stored at 27 deg C after being submitted to five different treatments. After hygienization, four out of five samples were exposed to gamma-ray at doses of 0.25 and 0.50 kGy. The unirradiated fruits were left for control and half of the irradiated samples were then climatized by exposure to CaC{sub 2}. Measurements of fresh mass loss, total soluble solids, peel coloration and disease index were performed on five different dates for up to nine days in order to monitor quality and the degree of ripening. It was found that high temperatures prevailing during climatization and storage accelerated maturation in all fruits in spite of the use of irradiation, with the control becoming ripe in 3 days only. In addition, based on the consistent results from the experiments performed, it can be concluded that irradiation at the doses tested was unable to overcome the maturation effects produced by climatization. However, the sole use of irradiation with 0.25 kGy yielded the lowest figures for fresh mass loss and disease index in the samples, thus becoming the most attractive among the treatments tested. (author)

  12. Maternal inheritance of chloroplast genome and paternal inheritance of mitochondrial genome in bananas (Musa acuminata).

    Science.gov (United States)

    Fauré, S; Noyer, J L; Carreel, F; Horry, J P; Bakry, F; Lanaud, C

    1994-03-01

    Restriction fragment length polymorphisms (RFLPs) were used as markers to determine the transmission of cytoplasmic DNA in diploid banana crosses. Progenies from two controlled crosses were studied with heterologous cytoplasmic probes. This analysis provided evidence for a strong bias towards maternal transmission of chloroplast DNA and paternal transmission of mitochondrial DNA in Musa acuminata. These results suggest the existence of two separate mechanisms of organelle transmission and selection, but no model to explain this can be proposed at the present time. Knowledge of the organelle mode of inheritance constitutes an important point for phylogeny analyses in bananas and may offer a powerful tool to confirm hybrid origins. PMID:7923414

  13. Repetitive part of the banana (Musa acuminata genome investigated by low-depth 454 sequencing

    Directory of Open Access Journals (Sweden)

    Hřibová Eva

    2010-09-01

    Full Text Available Abstract Background Bananas and plantains (Musa spp. are grown in more than a hundred tropical and subtropical countries and provide staple food for hundreds of millions of people. They are seed-sterile crops propagated clonally and this makes them vulnerable to a rapid spread of devastating diseases and at the same time hampers breeding improved cultivars. Although the socio-economic importance of bananas and plantains cannot be overestimated, they remain outside the focus of major research programs. This slows down the study of nuclear genome and the development of molecular tools to facilitate banana improvement. Results In this work, we report on the first thorough characterization of the repeat component of the banana (M. acuminata cv. 'Calcutta 4' genome. Analysis of almost 100 Mb of sequence data (0.15× genome coverage permitted partial sequence reconstruction and characterization of repetitive DNA, making up about 30% of the genome. The results showed that the banana repeats are predominantly made of various types of Ty1/copia and Ty3/gypsy retroelements representing 16 and 7% of the genome respectively. On the other hand, DNA transposons were found to be rare. In addition to new families of transposable elements, two new satellite repeats were discovered and found useful as cytogenetic markers. To help in banana sequence annotation, a specific Musa repeat database was created, and its utility was demonstrated by analyzing the repeat composition of 62 genomic BAC clones. Conclusion A low-depth 454 sequencing of banana nuclear genome provided the largest amount of DNA sequence data available until now for Musa and permitted reconstruction of most of the major types of DNA repeats. The information obtained in this study improves the knowledge of the long-range organization of banana chromosomes, and provides sequence resources needed for repeat masking and annotation during the Musa genome sequencing project. It also provides sequence

  14. Extraction of Cellulose from Kepok Banana Peel (Musa parasidiaca L.) for Adsorption Procion Dye

    OpenAIRE

    Poedji Loekitowati Hariani; Fahma Riyanti; Riski Dita Asmara

    2016-01-01

    The aim of the research was to extract a cellulose from kepok banana peel (Musa parasidiaca L.) and application to removal Procion dye. The extracted cellulose was prepared by dewaxing process to releasing of lignin, bleaching and hemicellulose removal. The cellulose identified by FTIR and SEM-EDS. The efficiency of the cellulose to adsorp Procion dye was evaluated by variation in the initial concentration of dye (5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 mg/L), solution pH (3, 4, 5, 6, 7, 8 a...

  15. MusaKäsi : Musiikki ja käsityö käsikädessä, toiminnallinen kokeilu musiikkileikkikoulussa

    OpenAIRE

    Mäki-Ikola, Annika

    2011-01-01

    Tämä opinnäytetyö perustuu MusaKäsi -kokeiluun, musiikin ja käsitöiden yhdistämiseen musiikkileikkikoulussa. MusaKäsi-kokeilun tarkoituksena oli tehdä kokeilu siitä, miten musiikki ja käsityöt kulkisivat samaan suuntaan musiikkileikkikoulussa. Tarkoituksena oli havainnoida myös, miten musiikki ja käsityöt saadaan toimivaksi kokonaisuudeksi osaksi musiikkileikkikoulun toimintaa. MusaKäsi -kokeilu toteutettiin syksyllä 2010 Etelä-Pohjanmaan musiikkiopiston Tanelinrannan opetuspisteen esikou...

  16. Incidência de fungos em pós-colheita de banana 'Prata anã'(Musa AAB Incidence of mushroons in post harvest of banana (Musa spp. 'Prata Anã' (AAB

    Directory of Open Access Journals (Sweden)

    Wilson da Silva Moraes

    2006-03-01

    Full Text Available Este trabalho teve por objetivo determinar a ocorrência e a freqüência de fungos em banana 'Prata anã' e elucidar o agente causal das podridões em pós-colheita de frutos provenientes do norte de Minas Gerais. Dois métodos de isolamento foram adotados: diluição em placas, a partir da lavagem de frutos verdes, e direto de frutos maduros. Os fungos Colletotrichum musae, Trichoderma harzianum, Fusarium equisetii, Penicillium sp. Aspergillus parasiticus, Trichothecium roseum, Colletotrichum acutatum, Alternaria sp., Cladosporium musae e Curvularia lunata foram os mais freqüentemente associados aos frutos. A patogenicidade desses fungos foi testada pela substituição de discos da casca de frutos verdes por discos de micélio. Colletotrichum musae apresentou área média lesionada em torno do ponto de inoculação igual a 5,8 cm², enquanto para os demais fungos testados não passou de 1,50 cm². Os resultados mostraram que C. musae é o agente primário das podridões dos frutos examinados com 100 % de incidência e os demais fungos limitaram-se a necrosar os ferimentos em torno do ponto de inoculação. O modo de infecção latente, causada por C. musae, parece favorecer, primeiramente, a colonização interna dos tecidos e, posteriormente, a ação dos fungos oportunistas, que aceleram as podridões nos frutos e na coroa.The objetive of this work were to determine the occurrence and frequency of fungus in 'Prata anã' banana and to elucidate the causal agent of post harvest rots on fruits from Jaíba, Minas Gerais, Brazil. Two isolation methods were used: diluition in placs from whashed green fruits and direct of mature fruits. The fungus Colletotrichum musae, Trichoderma harzianum, Fusarium equisetii, Penicillium sp., Aspergillus parasiticus, Trichothecium roseum, Colletotrichum acutatum, Alternaria sp., Cladosporium musae and Curvularia lunata were the more freqüentilly associated to fruits. The fungus pathogenicity were tested by

  17. A new isochroman-4-one derivative from the peel of Musa sapientum L. and its total synthesis

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    A new isochroman-4-one, 7,8-dihydroxy-3-methylisochroman-4-one was isolated from water soluble fraction of Musa sapientum L. Its structure was determined by spectroscopic evidences and its total synthesis has also been reported. The compound showed potent antihypertensive activity.

  18. Annotation of Differentially Expressed Genes in the Somatic Embryogenesis of Musa and Their Location in the Banana Genome

    Directory of Open Access Journals (Sweden)

    Josefina Ines Maldonado-Borges

    2013-01-01

    Full Text Available Analysis of cDNA-AFLP was used to study the genes expressed in zygotic and somatic embryogenesis of Musa acuminata Colla ssp. malaccensis, and a comparison was made between their differential transcribed fragments (TDFs and the sequenced genome of the double haploid- (DH- Pahang of the malaccensis subspecies that is available in the network. A total of 253 transcript-derived fragments (TDFs were detected with apparent size of 100–4000 bp using 5 pairs of AFLP primers, of which 21 were differentially expressed during the different stages of banana embryogenesis; 15 of the sequences have matched DH-Pahang chromosomes, with 7 of them being homologous to gene sequences encoding either known or putative protein domains of higher plants. Four TDF sequences were located in all Musa chromosomes, while the rest were located in one or two chromosomes. Their putative individual function is briefly reviewed based on published information, and the potential roles of these genes in embryo development are discussed. Thus the availability of the genome of Musa and the information of TDFs sequences presented here opens new possibilities for an in-depth study of the molecular and biochemical research of zygotic and somatic embryogenesis of Musa.

  19. Annotation of differentially expressed genes in the somatic embryogenesis of musa and their location in the banana genome.

    Science.gov (United States)

    Maldonado-Borges, Josefina Ines; Ku-Cauich, José Roberto; Escobedo-Graciamedrano, Rosa Maria

    2013-01-01

    Analysis of cDNA-AFLP was used to study the genes expressed in zygotic and somatic embryogenesis of Musa acuminata Colla ssp. malaccensis, and a comparison was made between their differential transcribed fragments (TDFs) and the sequenced genome of the double haploid- (DH-) Pahang of the malaccensis subspecies that is available in the network. A total of 253 transcript-derived fragments (TDFs) were detected with apparent size of 100-4000 bp using 5 pairs of AFLP primers, of which 21 were differentially expressed during the different stages of banana embryogenesis; 15 of the sequences have matched DH-Pahang chromosomes, with 7 of them being homologous to gene sequences encoding either known or putative protein domains of higher plants. Four TDF sequences were located in all Musa chromosomes, while the rest were located in one or two chromosomes. Their putative individual function is briefly reviewed based on published information, and the potential roles of these genes in embryo development are discussed. Thus the availability of the genome of Musa and the information of TDFs sequences presented here opens new possibilities for an in-depth study of the molecular and biochemical research of zygotic and somatic embryogenesis of Musa. PMID:24027442

  20. Effect of coconut palm proximities and Musa spp. germplasm resistance to colonization by Raoiella indica (Acari: Tenuipalpidae)

    Science.gov (United States)

    Although coconut (Cocos nucifera L.) is the predominant host for Raoiella indica Hirst (Acari: Tenuipalpidae), false spider mite infestations do occur on bananas and plantains (Musa spp. Colla). Since its introduction, the banana and plantain industries have been negatively impacted to different deg...

  1. Morphological State as a Predictor for Reintervention and Mortality After EVAR for AAA

    Energy Technology Data Exchange (ETDEWEB)

    Ohrlander, Tomas [Eksjoe County Hospital (Sweden); Dencker, Magnus [Malmoe University Hospital, Department of Clinical Physiology and Nuclear Medicine (Sweden); Acosta, Stefan, E-mail: stefan.acosta@telia.com [Malmoe University Hospital, Vascular Center Malmoe-Lund (Sweden)

    2012-10-15

    Purpose: This study was designed to assess aorto-iliac morphological characteristics in relation to reintervention and all-cause long-term mortality in patients undergoing standard EVAR for infrarenal AAA. Methods: Patients treated with EVAR (Zenith{sup Registered-Sign} Stentgrafts, Cook) between May 1998 and February 2006 were prospectively enrolled in a computerized database where comorbidities and preoperative aneurysm morphology were entered. Reinterventions and mortality were checked until December 1, 2010. Median follow-up time was 68 months. Results: A total of 304 patients were included, of which 86% were men. Median age was 74 years. The reintervention rate was 23.4% (71/304). A greater diameter of the common iliac artery (p = 0.037; hazard ratio (HR) 1.037 [1.002-1.073]) was an independent factor for an increased number of reinterventions. The 30-day mortality rate was 3.0% (9/304). Aneurysm-related deaths due to AAA occurred in 4.9% (15/304). Five patients died due to a concomitant ruptured thoracic aortic aneurysm. The mortality until end of follow-up was 54.3% (165/304). The proportion of deaths caused by vascular diseases was 61.6%. The severity of angulation of the iliac arteries (p = 0.014; HR 1.018 [95% confidence interval (CI) 1.004-1.033]) and anemia (p = 0.044; HR 2.79 [95% CI 1.029-7.556]) remained as independent factors associated with all-cause long-term mortality. The crude reintervention-free survival rate at 1, 3, and 5 years was 84.5%, 64.8%, and 51.6%, respectively. Conclusions: The initial aorto-iliac morphological state in patients scheduled for standard EVAR for AAA seems to be strongly related to the need for reinterventions and long-term mortality.

  2. NASA Astrophysics E/PO Impact: NASA SOFIA AAA Program Evaluation Results

    Science.gov (United States)

    Harman, Pamela; Backman, Dana E.; Clark, Coral; Inverness Research Sofia Aaa Evaluation Team, Wested Sofia Aaa Evaluation Team

    2015-01-01

    SOFIA is an airborne observatory, studying the universe at infrared wavelengths, capable of making observations that are impossible for even the largest and highest ground-based telescopes. SOFIA also inspires the development of new scientific instrumentation and fosters the education of young scientists and engineers.SOFIA is an 80% - 20% partnership of NASA and the German Aerospace Center (DLR), consisting of an extensively modified Boeing 747SP aircraft carrying a reflecting telescope with an effective diameter of 2.5 meters (100 inches). The SOFIA aircraft is based at NASA Armstrong Flight Research Center, Building 703, in Palmdale, California. The Science Program and Outreach Offices are located at NASA Ames Research center. SOFIA is a program in NASA's Science Mission Directorate, Astrophysics Division.Data will be collected to study many different kinds of astronomical objects and phenomena, including star cycles, solar system formation, identification of complex molecules in space, our solar system, galactic dust, nebulae and ecosystems.Airborne Astronomy Ambassador (AAA) Program:The SOFIA Education and Communications program exploits the unique attributes of airborne astronomy to contribute to national goals for the reform of science, technology, engineering, and math (STEM) education, and to elevate public scientific and technical literacy.The AAA effort is a professional development program aspiring to improve teaching, inspire students, and inform the community. To date, 55 educators from 21 states; Cycles 0, 1 and 2; have completed their astronomy professional development and their SOFIA science flight experience. Evaluation has confirmed the program's positive impact on the teacher participants, on their students, and in their communities. The inspirational experience has positively impacted their practice and career trajectory. AAAs have incorporated content knowledge and specific components of their experience into their curricula, and have given

  3. Phytochemical Screening and Effect of Musa paradisiaca Stem Extrude on Rat Haematological Parameters

    Directory of Open Access Journals (Sweden)

    Paul C. Onyenekwe

    2013-01-01

    Full Text Available This study was carried out to investigate the phytochemical composition and effect of various concentrations of Musa paradisiaca stem extrude on haematological parameters in Albino Wistar rats. Twenty rats (62-121 g were randomly assigned into 5 groups of 4 rats each. Group 1 the control group, were given ordinary water while the test groups 2, 3, 4 and 5 were given 25, 50, 75 and 100% of the aqueous extract, respectively without water for 28 days. Rats were sacrificed and blood samples were collected by cardiac puncture then used for the haematological studies. Phytochemical tests were carried out using standard laboratory techniques. The results of the study show the presence of tannins and glycosides in abundance in the stem extrude while saponins, flavonoids, alkaloids, polyphenols and reducing sugars were present in moderate amounts but phlobatannins was absent. There was a significant (p>0.05 increase in rat RBC, PCV, Hb and WBC counts at concentrations of 75 and 100% when compared with the control and a significant (p>0.05 decrease in MCH and MCHC. The levels of MCV were not significantly altered at all extract concentrations. It can therefore be concluded thatMusa paradisiaca stem extrude has a haematopoietic and immunomodulatory effect consistent with its ethnomedicinal use.

  4. Anti-ulcer and ulcer healing potentials of Musa sapientum peel extract in the laboratory rodents

    Directory of Open Access Journals (Sweden)

    Samuel Adetunji Onasanwo

    2013-01-01

    Full Text Available Background: This study investigated the anti-ulcer and ulcer healing potentials of the methanol extract of Musa sapientum peel in the laboratory rats. Materials and Methods: Methanol extract of the peels on Musa sapientum (MEMS was evaluated for its anti-ulcer using alcohol-induced, aspirin-induced, and pyloric ligation-induced models, and for its ulcer healing employing acetic acid-induced ulcer models in rats. Results: The findings from this experiment showed that MEMS (50, 100 and 200 mg/kg, b.w. anti-ulcer and ulcer healing activity (P ≤ 0.05 is dose-dependent. Also, MEMS exhibited healing of the ulcer base in all the treated groups when compared with the control group. Conclusion: The outcomes of this experiment revealed that the anti-ulcer effect of MEMS may be due to its anti-secretory and cyto-protective activity. The healing of the ulcer base might not be unconnected with basic fibroblast growth factors responsible for epithelial regeneration.

  5. Socioeconomic importance of the banana tree (Musa spp.) in the Guinean Highland Savannah agroforests.

    Science.gov (United States)

    Mapongmetsem, Pierre Marie; Nkongmeneck, Bernard Aloys; Gubbuk, Hamide

    2012-01-01

    Home gardens are defined as less complex agroforests which look like and function as natural forest ecosystems but are integrated into agricultural management systems located around houses. Investigations were carried out in 187 households. The aim of the study was to identify the different types of banana home gardens existing in the periurban zone of Ngaoundere town. The results showed that the majority of home gardens in the area were very young (less than 15 years old) and very small in size (less than 1 ha). Eleven types of home gardens were found in the periurban area of Ngaoundere town. The different home garden types showed important variations in all their structural characteristics. Two local species of banana are cultivated in the systems, Musa sinensis and Musa paradisiaca. The total banana production is 3.57 tons per year. The total quantity of banana consumed in the periurban zone was 3.54 tons (93.5%) whereas 1.01 tons were sold in local or urban markets. The main banana producers belonged to home gardens 2, 4, 7, and 9. The quantity of banana offered to relatives was more than what the farmers received from others. Farmers, rely on agroforests because the flow of their products helps them consolidate friendship and conserve biodiversity at the same time. PMID:22629136

  6. In-depth proteomic analysis of banana (Musa spp.) fruit with combinatorial peptide ligand libraries.

    Science.gov (United States)

    Esteve, Clara; D'Amato, Alfonsina; Marina, María Luisa; García, María Concepción; Righetti, Pier Giorgio

    2013-01-01

    Musa ssp. is among the world's leading fruit crops. Although a strong interest on banana biochemistry exists in the scientific community, focused on metabolite composition, proteins have been scarcely investigated even if they play an important role in food allergy and stability, are a source of biologically active peptides, and can provide information about nutritional aspects of this fruit. In this work we have employed the combinatorial peptide ligand libraries after different types of protein extractions, for searching the very low-abundance proteins in banana. The use of advanced MS techniques and Musa ssp. mRNAs database in combination with the Uniprot_viridiplantae database allowed us to identify 1131 proteins. Among this huge amount of proteins we found several already known allergens such as Mus a 1, pectinesterase, superoxide dismutase, and potentially new allergens. Additionally several enzymes involved in degradation of starch granules and strictly correlated to ripening stage were identified. This is the first in-depth exploration of the banana fruit proteome and one of the largest descriptions of the proteome of any vegetable system. PMID:23161558

  7. SU-E-P-16: A Feasibility Study of Using Eclipse AAA for SRS Treatement

    Energy Technology Data Exchange (ETDEWEB)

    Lim, S; LoSasso, T [Memorial Sloan-Kettering Cancer Center, New York, NY (United States)

    2015-06-15

    Purpose: To commission Varian Eclipse AAA for SRS treatment and compare the accuracy with Brainlab iPlan system for clinical cases measured with radiochromic film. Methods: A 6MV AAA clinical model for a Varian TrueBeam STx is used as baseline. The focal spot and field size of the baseline model(BASE) are (1.75,0.75) and 40×40cm{sup 2} respectively. Maximum field sizes, output factors(S{sub t}), FWHM focal spot and secondary source sizes are systematically adjusted to obtain an optimized model(OPT) by comparing the calculated PDD’s, profiles, and output factors with measurements taken with a stereotactic diode(SD) and, cc01 and cc04 ion chambers in Blue Phantom. In-phantom dose distributions of clinical SRS fields are calculated using the OPT and the clinical Brainlab iPlan pencil-beam. Within the 90% isodose-line(ROI), the average dose difference between the calculations and radiochromic film measurements are assessed. Results: The maximum field, focal spot and secondary source sizes for the OPT are 15×15cm{sup 2}, (0,0), and 32.3mm respectively. The OPT St input at 1.0 and 2.0cm fields are increased by 4.5% and 1.5% from BASE. The calculated output of the BASE and OPT underestimate by 16.1%–3.2% respectively at 0.5×0.5cm{sup 2} field and 3.1%−0.02% respectively at 1.0×1.0cm{sup 2} field. The depth doses at 10cm are within 3.5% and 0.4% of measurements for 0.5×0.5 and 1.0×1.0cm{sup 2}. The ROI dose of OPT and iPlan are within 1.6% and 0.6% of film measurements for 3.0cm clinical fields. For 1.0cm fields, the ROI dose of OPT underestimate 0.0–2.0% and iPlan overestimates 1.7–2.9% relative to measurements. Conclusion: The small field dose calculation of Eclipse AAA algorithm can be significantly improved by carefully adjusting the input parameters. The larger deviation of the OPT for 0.5×0.5cm{sup 2} field from measurements can be attributed to the lowest 1.0cm field size input limit of AAA. The OPT compares reasonably well with the iPlan pencil

  8. AAA+ Chaperone ClpX Regulates Dynamics of Prokaryotic Cytoskeletal Protein FtsZ*

    OpenAIRE

    Sugimoto, Shinya; Yamanaka, Kunitoshi; Nishikori, Shingo; Miyagi, Atsushi; Ando, Toshio; Ogura, Teru

    2009-01-01

    AAA+ chaperone ClpX has been suggested to be a modulator of prokaryotic cytoskeletal protein FtsZ, but the details of recognition and remodeling of FtsZ by ClpX are largely unknown. In this study, we have extensively investigated the nature of FtsZ polymers and mechanisms of ClpX-regulated FtsZ polymer dynamics. We found that FtsZ polymerization is inhibited by ClpX in an ATP-independent manner and that the N-terminal domain of ClpX plays a crucial role for the inhibition of FtsZ polymerizati...

  9. Single-molecule denaturation and degradation of proteins by the AAA+ ClpXP protease

    OpenAIRE

    Shin, Yongdae; Davis, Joseph H.; Brau, Ricardo R.; Martin, Andreas; Kenniston, Jon A.; Baker, Tania A.; Sauer, Robert T.; Lang, Matthew J.

    2009-01-01

    ClpXP is an ATP-fueled molecular machine that unfolds and degrades target proteins. ClpX, an AAA+ enzyme, recognizes specific proteins, and then uses cycles of ATP hydrolysis to denature any native structure and to translocate the unfolded polypeptide into ClpP for degradation. Here, we develop and apply single-molecule fluorescence assays to probe the kinetics of protein denaturation and degradation by ClpXP. These assays employ a single-chain variant of the ClpX hexamer, linked via a single...

  10. SU-E-P-16: A Feasibility Study of Using Eclipse AAA for SRS Treatement

    International Nuclear Information System (INIS)

    Purpose: To commission Varian Eclipse AAA for SRS treatment and compare the accuracy with Brainlab iPlan system for clinical cases measured with radiochromic film. Methods: A 6MV AAA clinical model for a Varian TrueBeam STx is used as baseline. The focal spot and field size of the baseline model(BASE) are (1.75,0.75) and 40×40cm2 respectively. Maximum field sizes, output factors(St), FWHM focal spot and secondary source sizes are systematically adjusted to obtain an optimized model(OPT) by comparing the calculated PDD’s, profiles, and output factors with measurements taken with a stereotactic diode(SD) and, cc01 and cc04 ion chambers in Blue Phantom. In-phantom dose distributions of clinical SRS fields are calculated using the OPT and the clinical Brainlab iPlan pencil-beam. Within the 90% isodose-line(ROI), the average dose difference between the calculations and radiochromic film measurements are assessed. Results: The maximum field, focal spot and secondary source sizes for the OPT are 15×15cm2, (0,0), and 32.3mm respectively. The OPT St input at 1.0 and 2.0cm fields are increased by 4.5% and 1.5% from BASE. The calculated output of the BASE and OPT underestimate by 16.1%–3.2% respectively at 0.5×0.5cm2 field and 3.1%−0.02% respectively at 1.0×1.0cm2 field. The depth doses at 10cm are within 3.5% and 0.4% of measurements for 0.5×0.5 and 1.0×1.0cm2. The ROI dose of OPT and iPlan are within 1.6% and 0.6% of film measurements for 3.0cm clinical fields. For 1.0cm fields, the ROI dose of OPT underestimate 0.0–2.0% and iPlan overestimates 1.7–2.9% relative to measurements. Conclusion: The small field dose calculation of Eclipse AAA algorithm can be significantly improved by carefully adjusting the input parameters. The larger deviation of the OPT for 0.5×0.5cm2 field from measurements can be attributed to the lowest 1.0cm field size input limit of AAA. The OPT compares reasonably well with the iPlan pencil-beam and measurements

  11. Estimating overdiagnosis in Screening for Abdominal Aortic Aneurysm: could a change in smoking habits and lowered aortic diameter tip the balance of AAA screening towards harm?

    DEFF Research Database (Denmark)

    Johansson, Minna; Hansson, Anders; Brodersen, John

    2015-01-01

    overdiagnosis are not available and unlikely to emerge. The psychosocial consequences of living with a screen detected AAA are inadequately investigated. Cost effectiveness data on screening are inconclusive Conclusion— Screening programmes have changed the meaning of an AAA diagnosis from a life threatening......Summary box Clinical context—Abdominal aortic aneurysms (AAAs) are often asymptomatic until they rupture, when the death rate is greater than 80%. If diagnosed before rupture, AAA can be treated with surgery, which has a mortality of 4-5% Diagnostic change— Sweden, the UK, and the US have initiated...

  12. Influence of the Oil Pequi in the Growth Mycelial and Sporulation of Colletotrichum Musae and Growth Micelial Of Rhizopus sp. in vitro.

    OpenAIRE

    B. L. Mendes; I. B. Zanatto; Ribeiro, A.S.; M. Pastorello; S. M. Bonaldo

    2013-01-01

    Abstract: The anthracnose caused by Colletotrichum musae and soft rot caused by Rhizopus sp are post-harvest diseases that influenced quality of exported fruits. To control these diseases fungicides are used, but in light of these waste products in the fruit and the environment, alternative control methods such as using biofungicides have been studied. The objective of this study was to evaluate the effect of pequi oil on mycelia growth and sporulation of Colletotrichum musae and mycelia grow...

  13. Genome-Wide Computational Analysis of Musa Microsatellites: Classification, Cross-Taxon Transferability, Functional Annotation, Association with Transposons & miRNAs, and Genetic Marker Potential.

    Science.gov (United States)

    Biswas, Manosh Kumar; Liu, Yuxuan; Li, Chunyu; Sheng, Ou; Mayer, Christoph; Yi, Ganjun

    2015-01-01

    The development of organized, informative, robust, user-friendly, and freely accessible molecular markers is imperative to the Musa marker assisted breeding program. Although several hundred SSR markers have already been developed, the number of informative, robust, and freely accessible Musa markers remains inadequate for some breeding applications. In view of this issue, we surveyed SSRs in four different data sets, developed large-scale non-redundant highly informative therapeutic SSR markers, and classified them according to their attributes, as well as analyzed their cross-taxon transferability and utility for the genetic study of Musa and its relatives. A high SSR frequency (177 per Mbp) was found in the Musa genome. AT-rich dinucleotide repeats are predominant, and trinucleotide repeats are the most abundant in transcribed regions. A significant number of Musa SSRs are associated with pre-miRNAs, and 83% of these SSRs are promising candidates for the development of therapeutic SSR markers. Overall, 74% of the SSR markers were polymorphic, and 94% were transferable to at least one Musa spp. Two hundred forty-three markers generated a total of 1047 alleles, with 2-8 alleles each and an average of 4.38 alleles per locus. The PIC values ranged from 0.31 to 0.89 and averaged 0.71. We report the largest set of non-redundant, polymorphic, new SSR markers to be developed in Musa. These additional markers could be a valuable resource for marker-assisted breeding, genetic diversity and genomic studies of Musa and related species. PMID:26121637

  14. Dissection of Axial-Pore Loop Function during Unfolding and Translocation by a AAA+ Proteolytic Machine

    Directory of Open Access Journals (Sweden)

    Ohad Iosefson

    2015-08-01

    Full Text Available In the axial channels of ClpX and related hexameric AAA+ protein-remodeling rings, the pore-1 loops are thought to play important roles in engaging, mechanically unfolding, and translocating protein substrates. How these loops perform these functions and whether they also prevent substrate dissociation to ensure processive degradation by AAA+ proteases are open questions. Using ClpX pore-1-loop variants, single-molecule force spectroscopy, and ensemble assays, we find that the six pore-1 loops function synchronously to grip and unfold protein substrates during a power stroke but are not important in preventing substrate slipping between power strokes. The importance of grip strength is task dependent. ClpX variants with multiple mutant pore-1 loops translocate substrates as well as the wild-type enzyme against a resisting force but show unfolding defects and a higher frequency of substrate release. These problems are magnified for more mechanically stable target proteins, supporting a threshold model of substrate gripping.

  15. Going the distance: validation of Acuros and AAA at an extended SSD of 400 cm.

    Science.gov (United States)

    Lamichhane, Narottam; Patel, Vivek N; Studenski, Matthew T

    2016-01-01

    Accurate dose calculation and treatment delivery is essential for total body irradia-tion (TBI). In an effort to verify the accuracy of TBI dose calculation at our institu-tion, we evaluated both the Varian Eclipse AAA and Acuros algorithms to predict dose distributions at an extended source-to-surface distance (SSD) of 400 cm. Measurements were compared to calculated values for a 6 MV beam in physical and virtual phantoms at 400 cm SSD using open beams for both 5 × 5 and 40 × 40cm2 field sizes. Inline and crossline profiles were acquired at equivalent depths of 5 cm, 10 cm, and 20 cm. Depth-dose curves were acquired using EBT2 film and an ion chamber for both field sizes. Finally, a RANDO phantom was used to simulate an actual TBI treatment. At this extended SSD, care must be taken using the planning system as there is good relative agreement between measured and calculated profiles for both algorithms, but there are deviations in terms of the absolute dose. Acuros has better agreement than AAA in the penumbra region. PMID:27074473

  16. OGLE16aaa - a Signature of a Hungry Super Massive Black Hole

    CERN Document Server

    Wyrzykowski, Łukasz; Kostrzewa-Rutkowska, Z; Hamanowicz, A; Jonker, P G; Arcavi, I; Guillochon, J; Brown, P J; Kozłowski, S; Udalski, A; Szymański, M K; Soszyński, I; Poleski, R; Pietrukowicz, P; Skowron, J; Mróz, P; Ulaczyk, K; Pawlak, M; Rybicki, K A; Greiner, J; Krühler, T; Bolmer, J

    2016-01-01

    We present the discovery and first three months of follow-up observations of a currently on-going unusual transient detected by the OGLE-IV survey, located in the centre of a galaxy at redshift z=0.1655. The long rise to absolute magnitude of -20.5 mag, slow decline, very broad He and H spectral features make OGLE16aaa similar to other optical/UV Tidal Disruption Events (TDEs). Weak narrow emission lines in the spectrum and archival photometric observations suggest the host galaxy is a weak-line Active Galactic Nucleus (AGN), which has been accreting at higher rate in the past. OGLE16aaa, along with SDSS J0748, seems to form a sub-class of TDEs by weakly or recently active supermassive black holes (SMBHs). This class might bridge the TDEs by quiescent SMBHs and flares observed as "changing-look QSOs", if we interpret the latter as TDEs. If this picture is true, the previously applied requirement for identifying a flare as a TDE that it had to come from an inactive nucleus, could be leading to observational bi...

  17. An atomic model AAA-ATPase/20S core particle sub-complex of the 26S proteasome.

    Science.gov (United States)

    Förster, Friedrich; Lasker, Keren; Beck, Florian; Nickell, Stephan; Sali, Andrej; Baumeister, Wolfgang

    2009-10-16

    The 26S proteasome is the most downstream element of the ubiquitin-proteasome pathway of protein degradation. It is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP consists of 6 AAA-ATPases and at least 13 non-ATPase subunits. Based on a cryo-EM map of the 26S proteasome, structures of homologs, and physical protein-protein interactions we derive an atomic model of the AAA-ATPase-CP sub-complex. The ATPase order in our model (Rpt1/Rpt2/Rpt6/Rpt3/Rpt4/Rpt5) is in excellent agreement with the recently identified base-precursor complexes formed during the assembly of the RP. Furthermore, the atomic CP-AAA-ATPase model suggests that the assembly chaperone Nas6 facilitates CP-RP association by enhancing the shape complementarity between Rpt3 and its binding CP alpha subunits partners. PMID:19653995

  18. An atomic model AAA-ATPase/20S core particle sub-complex of the 26S proteasome

    International Nuclear Information System (INIS)

    The 26S proteasome is the most downstream element of the ubiquitin-proteasome pathway of protein degradation. It is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP consists of 6 AAA-ATPases and at least 13 non-ATPase subunits. Based on a cryo-EM map of the 26S proteasome, structures of homologs, and physical protein-protein interactions we derive an atomic model of the AAA-ATPase-CP sub-complex. The ATPase order in our model (Rpt1/Rpt2/Rpt6/Rpt3/Rpt4/Rpt5) is in excellent agreement with the recently identified base-precursor complexes formed during the assembly of the RP. Furthermore, the atomic CP-AAA-ATPase model suggests that the assembly chaperone Nas6 facilitates CP-RP association by enhancing the shape complementarity between Rpt3 and its binding CP alpha subunits partners.

  19. An atomic model AAA-ATPase/20S core particle sub-complex of the 26S proteasome

    Energy Technology Data Exchange (ETDEWEB)

    Foerster, Friedrich [Department of Structural Biology, Max-Planck-Institute of Biochemistry, D-82152 Martinsried (Germany); Department of Bioengineering and Therapeutic Sciences, Department of Pharmaceutical Chemistry, and California Institute for Quantitative Biosciences (QB3), University of California at San Francisco, San Francisco (United States); Lasker, Keren [Department of Bioengineering and Therapeutic Sciences, Department of Pharmaceutical Chemistry, and California Institute for Quantitative Biosciences (QB3), University of California at San Francisco, San Francisco (United States); Blavatnik School of Computer Science, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Tel Aviv (Israel); Beck, Florian; Nickell, Stephan [Department of Structural Biology, Max-Planck-Institute of Biochemistry, D-82152 Martinsried (Germany); Sali, Andrej [Department of Bioengineering and Therapeutic Sciences, Department of Pharmaceutical Chemistry, and California Institute for Quantitative Biosciences (QB3), University of California at San Francisco, San Francisco (United States); Baumeister, Wolfgang, E-mail: baumeist@biochem.mpg.de [Department of Structural Biology, Max-Planck-Institute of Biochemistry, D-82152 Martinsried (Germany)

    2009-10-16

    The 26S proteasome is the most downstream element of the ubiquitin-proteasome pathway of protein degradation. It is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP consists of 6 AAA-ATPases and at least 13 non-ATPase subunits. Based on a cryo-EM map of the 26S proteasome, structures of homologs, and physical protein-protein interactions we derive an atomic model of the AAA-ATPase-CP sub-complex. The ATPase order in our model (Rpt1/Rpt2/Rpt6/Rpt3/Rpt4/Rpt5) is in excellent agreement with the recently identified base-precursor complexes formed during the assembly of the RP. Furthermore, the atomic CP-AAA-ATPase model suggests that the assembly chaperone Nas6 facilitates CP-RP association by enhancing the shape complementarity between Rpt3 and its binding CP alpha subunits partners.

  20. Antifungal Activities of Crude Extractum from Camellia semiserrata Chi (Nanshancha) Seed Cake Against Colletotrichum musae, Colletotrichum gloeosporioides and Penicillium italicum in vitro and in vivo Fruit Test.

    Science.gov (United States)

    Meng, Xiangchun; Li, Jun; Bi, Fangcheng; Zhu, Lixue; Ma, Zhiyu

    2015-12-01

    Antifungal activities of crude extractum of Nanshancha Seed Cake (NSC), to inactivate postharvest pathogens were investigated. Highest inhibitory rate was found against C. musae, C. gloeosporioides and C. papaya P.Henn, which was much stronger than that by tea saponin. Compared to tea saponin, effects of NSC extractum was relatively weak and similar on C. gloeosporioides Penzig and P. italicum. In an in vivo study, best controlling effects by NSC extractum was found with banana anthracnose disease development, which showed no inhibitory effects by tea saponin. NSC extractum controlled in vitro C. musae growth through directly inhibiting germination rate and germ tube elongation, and causing distortation, rupture and indentation of C. musae mycelium. In banana fruit subject to C. musae inoculation, higher PAL, POD, GLU and CHT activity was observed in banana fruit treated with crude NSC extractum than that of water control fruits. Current study proved the best controlling effects of crude NSC extractum in C. musae in vitro and in vivo development, which through direct inhibition of C. musae growth and increasing defense system of the banana fruit. PMID:26674222

  1. An atomic model AAA-ATPase/20S core particle sub-complex of the 26S proteasome

    OpenAIRE

    Förster, Friedrich; Lasker, Keren; Beck, Florian; Nickell, Stephan; Sali, Andrej; Baumeister, Wolfgang

    2009-01-01

    The 26S proteasome is the most downstream element of the ubiquitin-proteasome pathway of protein degradation. It is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP consists of 6 AAA-ATPases and at least 13 non-ATPase subunits. Based on a cryo-EM map of the 26S proteasome, structures of homologs, and physical protein-protein interactions we derive an atomic model of the AAA-ATPase-CP sub-complex. The ATPase order in our model (Rpt1/Rpt2/Rpt6/Rpt3/Rpt4/Rpt5) ...

  2. Analysis of positional candidate genes in the AAA1 susceptibility locus for abdominal aortic aneurysms on chromosome 19

    Directory of Open Access Journals (Sweden)

    Ferrell Robert E

    2011-01-01

    Full Text Available Abstract Background Abdominal aortic aneurysm (AAA is a complex disorder with multiple genetic risk factors. Using affected relative pair linkage analysis, we previously identified an AAA susceptibility locus on chromosome 19q13. This locus has been designated as the AAA1 susceptibility locus in the Online Mendelian Inheritance in Man (OMIM database. Methods Nine candidate genes were selected from the AAA1 locus based on their function, as well as mRNA expression levels in the aorta. A sample of 394 cases and 419 controls was genotyped for 41 SNPs located in or around the selected nine candidate genes using the Illumina GoldenGate platform. Single marker and haplotype analyses were performed. Three genes (CEBPG, PEPD and CD22 were selected for DNA sequencing based on the association study results, and exonic regions were analyzed. Immunohistochemical staining of aortic tissue sections from AAA and control individuals was carried out for the CD22 and PEPD proteins with specific antibodies. Results Several SNPs were nominally associated with AAA (p CEBPG, peptidase D (PEPD, and CD22. Haplotype analysis found a nominally associated 5-SNP haplotype in the CEBPG/PEPD locus, as well as a nominally associated 2-SNP haplotype in the CD22 locus. DNA sequencing of the coding regions revealed no variation in CEBPG. Seven sequence variants were identified in PEPD, including three not present in the NCBI SNP (dbSNP database. Sequencing of all 14 exons of CD22 identified 20 sequence variants, five of which were in the coding region and six were in the 3'-untranslated region. Five variants were not present in dbSNP. Immunohistochemical staining for CD22 revealed protein expression in lymphocytes present in the aneurysmal aortic wall only and no detectable expression in control aorta. PEPD protein was expressed in fibroblasts and myofibroblasts in the media-adventitia border in both aneurysmal and non-aneurysmal tissue samples. Conclusions Association testing

  3. Mammalian m-AAA Proteases as Key Regulators of Mitochondrial Function - Analysis of Dominant Negative Mutant Variants

    OpenAIRE

    Raschke, Ines

    2009-01-01

    To ensure the removal of excess and non-assembled proteins, mitochondria require a protein quality control system which is constituted by several proteases located in different compartments of mitochondria. m-AAA proteases, oligomeric ATP-dependent metallopeptidases, are key components of this system active at the matrix side of the inner membrane. Human m-AAA proteases build up homo- and hetero-oligomeric complexes composed of AFG3L2 and SPG7. Mice express a third subunit, Afg3l1, resulting ...

  4. SUSTRATOS FOLIARES PARA EL INCREMENTO DE BACTERIAS QUITINOLÍTICAS Y GLUCANOLÍTICAS EN LA FILOSFERA DE BANANO FOLIAR SUBSTRATES FOR INCREASING CHITINOLYTIC AND GLUCANOLYTIC BACTERIA ON PHYLLOSPHERE OF BANANA

    Directory of Open Access Journals (Sweden)

    Lina María Salazar Peláez

    2006-12-01

    Full Text Available La Sigatoka Negra, causada por el hongo Mycosphaerella fijiensis Morelet, es considerada la enfermedad de mayor importancia económica para los cultivos de musáceas alimenticias. Su control, se fundamenta en la aplicación de fungicidas sintéticos, cuyo modo de acción viene siendo vulnerado por poblaciones del hongo. La presente investigación, ha explorado la posibilidad de desarrollar una estrategia de regulación biológica, basada en el estudio y manipulación de algunos de los factores bióticos y abióticos que interactúan en la filosfera de banano, con el objetivo de incrementar las poblaciones epifitas naturales de bacterias quitinolíticas y glucanolíticas, las cuales pueden afectar negativamente la germinación del patógeno. Con base en una fase previa a este estudio, en la cual se realizó una caracterización químico-microbiológica parcial de la filosfera de plantas de banano y plátano bajo condiciones del Urabá antioqueño (Colombia, se evaluaron 14 sustratos foliares, compuestos por quitina coloidal (QC [4 %], harina de cebada (HC [2,5 %] y urea (U [1 %], solas ó en combinación, los cuales siempre estuvieron acompañados de una solución mineral base y una solución adherente-dispersante. Aquellos sustratos a base de HC y U, produjeron los mayores incrementos en las poblaciones de bacterias líticas epifitas, con recuentos superiores a las 10.000 veces, con respecto a sus poblaciones iniciales sobre hojas de banano del cultivar Gran Enano, y de hasta 8,6 y 15 veces mayores para las bacterias quitinolíticas y glucanolíticas respectivamente, con relación a la población en el tratamiento sin sustratos foliares. Se discute la influencia de la precipitación sobre las poblaciones de dichas bacterias líticas y la necesidad de ajustar una formulación que mejore las posibilidades de control sobre la sigatoka negra.Black Sigatoka, caused by the fungus Mycosphaerella fijiensis Morelet, is considered the economically most

  5. EVALUACIÓN DE RESISTENCIA DE GENOTIPOS DE PLÁTANO Y BANANO A LA SIGATOKA NEGRA (MYCOSPHAERELLA FIJIENSIS MORELET. EVALUATION OF RESISTANCE OF PLANTAIN AND BANANA GENOTYPES TO BLACK SIGATOKA (MYSCOSPHAERELLA FIJIENSIS MORELET.

    Directory of Open Access Journals (Sweden)

    Alejandra Cuéllar Quintero

    2011-06-01

    Full Text Available Con el objetivo de evaluar bajo condiciones de invernadero genotipos de plátano y banano frente a poblaciones de Mycosphaerella fijiensis Morelet de diferente virulencia y origen, se estableció una colección de 125 aislamientos monospóricos obtenidos en 10 departamentos de Colombia. Se seleccionaron 50 aislamientos por área geográfica y genotipo hospedante, para la caracterización morfológica y patogénica del hongo en Dominico Hartón. En las pruebas de patogenicidad y resistencia se realizaron inoculaciones de una suspensión acuosa de 5.000 conidias mL-1, la respuesta de las plántulas a la enfermedad se determinó mediante las variables periodo de incubación (PI, tiempo de evolución de los síntomas (TES, área bajo la curva del desarrollo de la enfermedad (ABCDE y tasa de desarrollo de la enfermedad (r. La inoculación de los 50 aislamientos en plántulas de Dominico Hartón, permitió establecer cinco niveles de virulencia (muy alta, alta, media, baja y muy baja. La virulencia de los aislamientos no se relacionó con su origen geográfico ni con el genotipo del cual se obtuvieron; encontrándose en una misma zona y en un mismo genotipo, aislamientos de diferente virulencia. Los genotipos de plátano y banano mostraron un comportamiento diferencial frente a cinco aislamientos de diferente virulencia y origen, resultados que permitieron establecer tres niveles de reacción de los genotipos frente a los aislamientos (resistente, intermedio y susceptible, destacándose los genotipos Topocho, Maqueño, FHIA 20, FHIA 21 de plátano y los genotipos Sedita y FHIA 23 de banano por presentar un mayor nivel de resistencia, expresada como un progreso lento y menos severo de la enfermedad.A study was conducted to evaluate plantain and banana genotypes with Mycosphaerella fijiensis Morelet isolates of different virulence and geographic origin. The pathogenicity test and morphological characterization of the M. fijiensis populations were

  6. Evaluation of vegetable extracts for control of the pathogenic fungi in banana and strawberry in post harvest storage Evaluación de extractos vegetales para manejo de hongos patógenos en banano y fresa almacenados

    Directory of Open Access Journals (Sweden)

    Bonilla Correa Carmen Rosa

    2006-12-01

    Full Text Available

    This study was carried out get knowledge about fungicidal properties of vegetable extracts to the control of the pathogenyc fungi (Colletotrichum musae and Botrytis cinerea in banana (Musa sapientum and strawberry (Fragaria sp during the pos harvest storage, at the National University of Colombia headquarters in Palmira. Ethanolics extracts of the following vegetable species were evaluated: Plantago major L, Ruta graveolens L, Lippia alba (Mill NE BROS, Pteridium aquilinum (Kaulf Maxon. The extracts were obtained by the percolation method starting from the macerated material (100 g and 1000 ml of solvent. The fungi was isolated from the strawberry (Fragaria sp affected by B. cinerea and banana Gross mitchel affected by C. musae. To evaluate the control of the fungi C. musae and B. cinerea sixteen treatments were used (four extracts of plants, two extraction media, two dilutions and three repetitions. Three control treatments were added: absolute (PDA; solvent (PDA + ethanol or water; and chemical (PDA + chemical fungicide. The dilutions were prepared with the solvent used to obtain the extract. The evaluation in vitro was maid with ethanolics extracts diluted at 25%, because these presented the best results in the previous tests. The results indicate that the ethanolic solvent decreased the development of the fungi in 68%. The fruits in which the etanolic extract of ruda and pronto alivio was applied presented bigger incidence and grade of severity.

    Keys words: Vegetable extract; patogenic fungi; Colletotrichum musae; Botrytis cinérea; post harvest

    En la Universidad Nacional de Colombia -Sede Palmira, se obtuvieron extractos etanólicos (1.000 ml, macerados (100 g de llantén (Plantago major L, ruda (Ruta graveolens L, pronto alivio [Lippia alba (Mill NE BROS] y helecho marranero [Pteridium aquilinum (Kaulf Maxon]. Para la evaluación del control

  7. Trapping Effect of Baxi Banana(Musa AAA Cavendish)Pseudostem on Two Banana Weevil Species%巴西蕉假茎对2种香蕉象甲的诱捕效果分析

    Institute of Scientific and Technical Information of China (English)

    李科明; 许桂莺; 彭正强

    2015-01-01

    This study is to determine the effect of Baxi banana pseudostem as attractant to two banana weevil species , and to provide theoretical guidance for control of banana weevil species. Field traps of pseudostem to banana weevils weredeployed for the analysis,meanwhile,indoor selection response of banana weevil to Baxi banana pseudostem was conducted by using double pitfall olfactometer. Significant trapping effects of Baxi banana pseudostem on two ba⁃nana weevils were found by field trapping and number of the trapped banana weevils in five and ten days reaching 8.3~11.3 and 14.7~18.0 individuals per trap,respectively.Indoor selection response results showed that both the two banana weevils showed significant selection effect to the Baxi banana pseudostem when compared with blank control. Baxi banana pseudostem could be used to control the two banana weevil species.%为明确巴西蕉假茎对香蕉假茎象甲和香蕉球茎象甲的诱捕效果,为利用巴西蕉假茎防治香蕉象甲这一农业防治措施提供理论依据,采用假茎田间诱捕试验及室内选择反应试验,研究了巴西蕉假茎对2种香蕉象甲的诱捕效果。田间诱捕试验结果表明,巴西蕉假茎对2种香蕉象甲具有有效的诱捕作用,其5d和10d的诱捕量分别达8.3~11.3和14.7~18.0头/诱捕器;室内选择反应试验结果表明,与空白对照相比,2种香蕉象甲对巴西蕉假茎均表现出显著的选择趋性。因此,巴西蕉假茎可用于蕉园香蕉象甲的诱捕防治。

  8. Avaliação de clones de bananeira do subgrupo Cavendish (Musa acuminata, AAA em Cruz das Almas-BA Evaluation of banana Cavendish clones in Cruz das Almas City, Bahia State, Brazil

    Directory of Open Access Journals (Sweden)

    Sebastião de Oliveira e Silva

    2004-12-01

    Full Text Available Foram avaliados em Cruz das Almas-BA, em dois ciclos, 69 clones Cavendish coletados em São Paulo, Minas Gerais, Bahia e Santa Catarina, sendo 49 do tipo Nanicão e 20 do tipo Grande Naine. Os clones foram estabelecidos em área experimental da Embrapa Mandioca e Fruticultura utilizando-se um delineamento em blocos ao acaso com cinco repetições e cinco plantas por parcela. Foram utilizados a irrigação por microaspersão, o espaçamento de 3,0 m x 2,0 m e um sistema de condução com três plantas/touceira. Os requerimentos agronômicos foram aplicados conforme preconizados para o cultivo. Na avaliação dos clones, foram consideradas as seguintes variáveis: altura da planta em centímetros (AP; número de dias do plantio à colheita (ND; peso do cacho em quilogramas (PC; número de frutos por cacho (NF; e comprimento do fruto em centímetros (CF. Pelos resultados obtidos, verificou-se uma variabilidade média em todas as variáveis avaliadas. Por apresentarem caracteres superiores, foram selecionados cinco clones de Grande Naine (G.N. Taperão, G.N.Rossete, G.N. Willians, G.N. Magário, G.N. SC-074 e quatro de Nanicão (N. IAC Abóbada Verde, N. Rossete, N. SC-0008 e N. SC-063.Sixty-nine clones of Cavendish type bananas (49 Nanicão and 20 Grande Naine collected in the States of São Paulo, Minas Gerais, Bahia and Santa Catarina were evaluated. The clones were established in an experimental area at the Cassava and Fruit Crops Research Center - EMBRAPA, using micro-aspersion irrigation, in plants spaced at 3.0 m x 2.0 m, and conducted with three plants/bushes. Random blocks were used with five replications and five plants per plot. All cultural requirements for the crop were carried out. For the clone evaluations, the following variables were considered: plant height in centimeters (PH; number of days from planting to yield (ND; weight of bunch in kilograms (WB; number of fruits per bunch (FB and length of fruit in centimeters (LF. The results obtained showed medium variability in all the characteristics evaluated and five clones of Grande Naine (G.N. Taperão, G.N.Rossete, G.N. Willians, G.N. Magário, G.N. SC-074 and four of Nanicão (N. IAC Green Vault, N. Rossete, N. SC-0008 and N. SC-063 were selected for presenting superior characteristics.

  9. Musa massif: mapping, petrology and petrochemical, Rio Maria, SE from Para State

    International Nuclear Information System (INIS)

    The petrological, geochemical and geochronological studies allow some insight on the genesis and evolution of the Musa Massif. The different facies of the granitic body are cogenetic, although each of these facies presents some peculiarities in its genesis and evolution. These data suggests that the granite magma evolution was complex or, alternatively, that the facies were generated by liquids derived from different sources. A model of magmatic emplacement, genesis and differentiation is proposed and discussed. The granitic facies show a calc-alkaline compositions, exhibiting strong analogies with cordilleran granites or magnetite granites. An age of 1692 +- 11 Ma (Rb/Sr) with IR of 0,70777 +- 0,00023 was obtained for different facies of Massif. A preliminary attempt to individualize geochronology the principal facies was done and showed that there is a coincidence between the ages and the emplacement sequence of these facies of the pluton. (author)

  10. Controlled green synthesis of silver nanoparticles by Allium cepa and Musa acuminata with strong antimicrobial activity

    Science.gov (United States)

    Sahni, Geetika; Panwar, Amit; Kaur, Balpreet

    2015-02-01

    A controlled "green synthesis" approach to synthesize silver nanoparticles by Allium cepa and Musa acuminata plant extract has been reported. The effect of different process parameters, such as pH, temperature and time, on synthesis of Ag nanoparticles from plant extracts has been highlighted. The work reports an easy approach to control the kinetics of interaction of metal ions with reducing agents, stabilized by ammonia to achieve sub-10 nm particles with narrow size distribution. The nanoparticles have been characterized by UV-Visible spectra and TEM analysis. Excellent antimicrobial activity at extremely low concentration of the nanoparticles was observed against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Fusarium oxysporum which may allow their exploitation as a new generation nanoproduct in biomedical and agricultural applications.

  11. Efeito do óleo de Piper aduncum no controle em pós-colheita de Colletotricum musae em banana The effect of essential oil of Piper aduncum in controlling Colletotrichum musae on post harvest bananas

    OpenAIRE

    Cleber N. Bastos; Paulo Sérgio B. Albuquerque

    2004-01-01

    Em pós-colheita, a podridão dos frutos causada por Colletotrichum musae é a doença mais importante da banana (Musa spp.). Testes in vitro e in vivo foram realizados com o objetivo de avaliar o efeito fungitóxico do óleo essencial de pimenta-de-macaco (Piper aduncum) sobre o patógeno. Nas concentrações acima de 100 µg/ml, o óleo inibiu, em 100%, o crescimento micelial e a germinação dos conídios. No teste in vivo foram usados frutos de banana "Prata", nos quais foram feitos orifícios com...

  12. Direct Effects Of Chronic Gamma Radiation On Musa Acuminata Var. Berangan, A Local Malaysia Banana Cultivar

    International Nuclear Information System (INIS)

    Musa acuminata var. Berangan, is a popular variety of our local banana known as Pisang Berangan. The variety is a triploid banana, use mainly for dessert and has a great value for commodity fruit crops. However, production of PisangBerangan has been threatened by diseases such as Fusarium wilt, black sigatoka, Fusarium wilt, burrowing nematodes and viral diseases like Banana streak virus, Banana bunch top virus and Banana bract mosaic virus. The scenario becoming worst as Musa has a narrow genetic background for breeding and/or selection program. The banana breeding program of edible bananas is hampered by high sterility, and very limited amounts of seeds. Mutation induction via chronic gamma radiation is an alternative ways in creating more variants for selections towards a better quality and disease tolerance. A total number of 75 samples at nursery stage (1 month) were exposed to chronic gamma radiation in Gamma Greenhouse at Malaysian Nuclear Agency for 28 weeks. The samples were accordingly arranged with distance ranging from 1 m to 15 m from gamma source (Cesium-137). Plant height and new buds were used as measurement parameters in evaluating the direct effects of the chronic gamma radiation. Results showed effective dose of chronic gamma radiation in Pisang Berangan was 20 Gy. Number of new emerging sucker was ranging from 1-3 pieces with the highest at ring-4 and ring-5. Plant height was observed ranging from 22.1 to 110.5 cm. Effects of chronic radiation were observed after 3-4 months in the GGH. The samples revealed as striking leaves, short inter node and new emergence of suckers. The objective of this work is to get a dose response for chronic gamma radiation in Pisang Berangan. As for selection of potential mutant variants, new emerging suckers were tissue cultured in segregating chimeras and to get required numbers of samples for further field evaluation. (author)

  13. Lipid Profile and Electrolyte Composition in Diabetic Rats Treated With Leaf Extract of Musa sapientum.

    Science.gov (United States)

    Adewoye, E O; Ige, A O

    2016-01-01

    Diabetes mellitus affects lipid levels resulting in diabetic dyslipidemia as well as electrolyte loss from the body. Musa sapientum has been reported to possess antidiabetic properties. This study assessed the lipid profile and electrolyte composition in alloxan-induced diabetic rats treated with methanol leaf extract of M. sapientum (cMEMSL). Diabetes was induced with alloxan (120 mg/kg i.p.). Seventy-five male albino rats were divided into 5 groups of 15 rats each. Group 1 was control; groups 2-5 were made diabetic and treated with 0.2 ml 0.9% NaCl, cMEMSL (250 mg/kg and 500 mg/kg), and glibenclamide (5 mg/kg), respectively, for 14 days. Blood samples were obtained from the retro orbital sinus after light anesthesia from 5 animals in each group on days 2, 7, and 14 for lipids and electrolyte analysis. Lipid profile of diabetic treated (cMEMSL and glibenclamide) animals showed significant reduction (p density lipoprotein (LDL) levels. The high density lipoprotein (HDL) level in the treatment groups increased significantly (p < .05) compared with diabetic untreated. Sodium, potassium, and phosphate ions significantly increased in all diabetic treatment groups while chloride ion significantly decreased compared with diabetic untreated. There was no significant difference in calcium and bicarbonate ion concentration in all the groups. This study has showed additional properties of Musa sapientum to include its ability to restore electrolyte balance, reduce cholesterol, triglyceride, LDL, and increase the HDL levels in diabetic animals. PMID:25320868

  14. Development of Green Banana (Musa paradisiaca as Potential Food Packaging Films and Coatings

    Directory of Open Access Journals (Sweden)

    Nur Hanani Z. A.

    2016-02-01

    Full Text Available The aim of this study was to develop biodegradable packaging films based on a unripe green banana (Musa paradisiaca L. with different plasticizers; glycerol, polyethylene glycol (PEG and sorbitol at various concentrations (10-50%. Banana films were produced by using casting method and physical properties of these films were determined. Banana films with 10% of PEG showed the lowest water solubility (P≤0.05 followed by films with glycerol and sorbitol. Banana films with 40% plasticizers possessed the lowest water vapor permeability (WVP whereas films with 30% glycerol exhibited higher values of tensile strength (P≤0.05 compared to films with PEG and sorbitol. However, types of plasticizers did not influence the thickness of the films. Also, used of higher concentrations of plasticizers had increased the solubility values. These findings reveal that concentrations and types of plasticizers have significant roles to provide banana film or coating with good physical properties. The aim of this study was to develop biodegradable packaging films based on a unripe green banana (Musa paradisiaca L. with different plasticizers; glycerol, polyethylene glycol (PEG and sorbitol at various concentrations (10-50%. Banana films were produced by using casting method and physical properties of these films were determined. Banana films with 10% of PEG showed the lowest water solubility (P≤0.05 followed by films with glycerol and sorbitol. Banana films with 40% plasticizers possessed the lowest water vapour permeability (WVP whereas films with 30% glycerol exhibited higher values of tensile strength (P≤0.05 compared to films with PEG and sorbitol. However, types of plasticizers did not influence the thickness of the films. Also, used of higher concentrations of plasticizers had increased the solubility values. These findings reveal that concentrations and types of plasticizers have significant roles to provide banana film or coating with good physical

  15. Multiplicación del clon de banano ‘FHIA-18’ (AAAB en Sistema de Inmersión Temporal

    Directory of Open Access Journals (Sweden)

    Milagros Basail Pérez

    2012-06-01

    Full Text Available Título en inglés: Multiplication of banana clone 'FHIA-18' (AAAB in Temporary Immersion System Normal 0 21 false false false ES-CO X-NONE X-NONE MicrosoftInternetExplorer4 Resumen Con el propósito de desarrollar un protocolo para la multiplicación del clon de banano ´FHIA-18´ (AAAB en sistema de inmersión temporal, se definieron como objetivos del trabajo determinar el efecto del tiempo (5, 10 y 15 minutos y la frecuencia de inmersión (3, 6 y 8 horas por día, así como la influencia de diferentes combinaciones de reguladores del crecimiento (2,0; 3,0 y  4,0 mg.L-1 de 6-BAP y 2,0; 2,5; 3,0; 3,5 y 4,0 mg.L-1 de 6 AIA,  el efecto del volumen de medio de cultivo por planta (20, 30, 40 y 50 ml/explante y la densidad de explantes por frasco de cultivo (30, 50, 70 y 90 explantes/frasco para incrementar el coeficiente de multiplicación. Con el empleo de un tiempo de 10 minutos y una frecuencia de inmersión cada tres horas, se alcanzaron los mejores resultados en cuanto al número de explantes obtenidos. Con este tiempo y frecuencia de inmersión los explantes presentaron el mayor diámetro del pseudotallo. Para cada frasco de 10,0 L se inocularon 70 explantes y la renovación con 2800 ml de medio de cultivo (40 ml/explante con un tiempo de cultivo de 21 días permitió alcanzar la mayor productividad del material en fase de multiplicación. Además al utilizar las sales MS suplementadas con 3,0 mg.L-1 de 6-BAP; 2,0 mg.L-1 de AIA; 10,0 mg.L-1 de ácido ascórbico, se logró disminuir el crecimiento innecesario de los tallos y hojas de los brotes en la fase de multiplicación y por lo tanto un mayor número de explantes. Palabras claves: coeficiente, densidad de explantes, tiempo, frecuencia, volumen de medio de cultivo. Abstract In order to develop a protocol for multiplication of Banana clone 'FHIA-18' (AAAB in temporary immersion systems, the following working objectives were defined: to determine the effect of immersion time (5, 10 and 15

  16. Simulation of bifurcated stent grafts to treat abdominal aortic aneurysms (AAA)

    CERN Document Server

    Egger, Jan; Freisleben, Bernd

    2016-01-01

    In this paper a method is introduced, to visualize bifurcated stent grafts in CT-Data. The aim is to improve therapy planning for minimal invasive treatment of abdominal aortic aneurysms (AAA). Due to precise measurement of the abdominal aortic aneurysm and exact simulation of the bifurcated stent graft, physicians are supported in choosing a suitable stent prior to an intervention. The presented method can be used to measure the dimensions of the abdominal aortic aneurysm as well as simulate a bifurcated stent graft. Both of these procedures are based on a preceding segmentation and skeletonization of the aortic, right and left iliac. Using these centerlines (aortic, right and left iliac) a bifurcated initial stent is constructed. Through the implementation of an ACM method the initial stent is fit iteratively to the vessel walls - due to the influence of external forces (distance- as well as balloonforce). Following the fitting process, the crucial values for choosing a bifurcated stent graft are measured, ...

  17. Health-related quality of life outcomes following elective open or endovascular AAA repair: a randomized controlled trial.

    NARCIS (Netherlands)

    Lottman, P.E.M.; Laheij, R.J.F.; Cuypers, P.W.; Bender, M.H.M.; Buth, J.

    2004-01-01

    PURPOSE: To assess health-related quality of life outcomes after endovascular versus open abdominal aortic aneurysm repair. METHODS: Participants were randomly assigned to receive either endovascular or open abdominal aortic aneurysm (AAA) surgery according to a rate of 3 endovascular patients to 1

  18. Clinical dosimetric impact of Acuros XB and analytical anisotropic algorithm (AAA on real lung cancer treatment plans : review

    Directory of Open Access Journals (Sweden)

    Suresh Rana

    2014-02-01

    Full Text Available Photon dose calculation algorithms in treatment planning system could affect the accuracy of dose delivery when tissue heterogeneity is involved along the beam path. Treatment planning for lung cancer is challenging, especially in the case of treatment plan involving small fields. The combination of low-density (air medium and small fields cause charge particle disequilibrium nears the air/tissue interface. Beam modeling within the dose calculation algorithms must also employ an accurate method of accounting tissue heterogeneity corrections in order to avoid dose overestimation or underestimation. Analytical anisotropic algorithm (AAA is one of the widely tested and validated dose calculation algorithms in external beam photon radiation therapy. Recently, Acuros XB (AXB was made available for photon dose calculations, and several studies have demonstrated better dose prediction accuracy of the AXB over AAA. This article reviews the results from the treatment planning studies, which have investigated the clinical dosimetric impact of the AXB and AAA on real lung cancer treatment plans.--------------------------------------Cite this article as: Rana S. Clinical dosimetric impact of Acuros XB and analytical anisotropic algorithm (AAA on real lung cancer treatment plans: review. Int J Cancer Ther Oncol 2014; 2(1:02019.DOI: http://dx.doi.org/10.14319/ijcto.0201.9

  19. The AAA-ATPase NVL2 is a telomerase component essential for holoenzyme assembly

    Energy Technology Data Exchange (ETDEWEB)

    Her, Joonyoung [Departments of Biology and Integrated Omics for Biomedical Science, Yonsei University, Seoul 120-749 (Korea, Republic of); Chung, In Kwon, E-mail: topoviro@yonsei.ac.kr [Departments of Biology and Integrated Omics for Biomedical Science, Yonsei University, Seoul 120-749 (Korea, Republic of)

    2012-01-20

    Highlights: Black-Right-Pointing-Pointer Identification of the AAA-ATPase NVL2 as a novel hTERT-interacting protein. Black-Right-Pointing-Pointer NVL2 associates with catalytically active telomerase via an interaction with hTERT. Black-Right-Pointing-Pointer NVL2 is a telomerase component essential for holoenzyme assembly. Black-Right-Pointing-Pointer ATP-binding activity of NVL2 is required for hTERT binding and telomerase assembly. -- Abstract: Continued cell proliferation requires telomerase to maintain functional telomeres that are essential for chromosome integrity. Although the core enzyme includes a telomerase reverse transcriptase (TERT) and a telomerase RNA component (TERC), a number of auxiliary proteins have been identified to regulate telomerase assembly, localization, and enzymatic activity. Here we describe the characterization of the AAA-ATPase NVL2 as a novel hTERT-interacting protein. NVL2 interacts and co-localizes with hTERT in the nucleolus. NLV2 is also found in association with catalytically competent telomerase in cell lysates through an interaction with hTERT. Depletion of endogenous NVL2 by small interfering RNA led to a decrease in hTERT without affecting the steady-state levels of hTERT mRNA, thereby reducing telomerase activity, suggesting that NVL2 is an essential component of the telomerase holoenzyme. We also found that ATP-binding activity of NVL2 is required for hTERT binding as well as telomerase assembly. Our findings suggest that NVL2, in addition to its role in ribosome biosynthesis, is essential for telomerase biogenesis and provides an alternative approach for inhibiting telomerase activity in cancer.

  20. Pareto front analysis of 6 and 15 MV dynamic IMRT for lung cancer using pencil beam, AAA and Monte Carlo

    Energy Technology Data Exchange (ETDEWEB)

    Ottosson, R O; Karlsson, A; Behrens, C F, E-mail: riolot01@heh.regionh.d [Department of Oncology (R), Division of Radiophysics (52AA), Copenhagen University Hospital Herlev, Herlev Ringvej 75, DK-2730 Herlev (Denmark)

    2010-08-21

    The pencil beam dose calculation method is frequently used in modern radiation therapy treatment planning regardless of the fact that it is documented inaccurately for cases involving large density variations. The inaccuracies are larger for higher beam energies. As a result, low energy beams are conventionally used for lung treatments. The aim of this study was to analyze the advantages and disadvantages of dynamic IMRT treatment planning for high and low photon energy in order to assess if deviating from the conventional low energy approach could be favorable in some cases. Furthermore, the influence of motion on the dose distribution was investigated. Four non-small cell lung cancer cases were selected for this study. Inverse planning was conducted using Varian Eclipse. A total number of 31 dynamic IMRT plans, distributed amongst the four cases, were created ranging from PTV conformity weighted to normal tissue sparing weighted. All optimized treatment plans were calculated using three different calculation algorithms (PBC, AAA and MC). In order to study the influence of motion, two virtual lung phantoms were created. The idea was to mimic two different situations: one where the GTV is located centrally in the PTV and another where the GTV was close to the edge of the PTV. PBC is in poor agreement with MC and AAA for all cases and treatment plans. AAA overestimates the dose, compared to MC. This effect is more pronounced for 15 than 6 MV. AAA and MC both predict similar perturbations in dose distributions when moving the GTV to the edge of the PTV. PBC, however, predicts results contradicting those of AAA and MC. This study shows that PB-based dose calculation algorithms are clinically insufficient for patient geometries involving large density inhomogeneities. AAA is in much better agreement with MC, but even a small overestimation of the dose level by the algorithm might lead to a large part of the PTV being underdosed. It is advisable to use low energy as a

  1. Pareto front analysis of 6 and 15 MV dynamic IMRT for lung cancer using pencil beam, AAA and Monte Carlo

    Science.gov (United States)

    Ottosson, R. O.; Karlsson, A.; Behrens, C. F.

    2010-08-01

    The pencil beam dose calculation method is frequently used in modern radiation therapy treatment planning regardless of the fact that it is documented inaccurately for cases involving large density variations. The inaccuracies are larger for higher beam energies. As a result, low energy beams are conventionally used for lung treatments. The aim of this study was to analyze the advantages and disadvantages of dynamic IMRT treatment planning for high and low photon energy in order to assess if deviating from the conventional low energy approach could be favorable in some cases. Furthermore, the influence of motion on the dose distribution was investigated. Four non-small cell lung cancer cases were selected for this study. Inverse planning was conducted using Varian Eclipse. A total number of 31 dynamic IMRT plans, distributed amongst the four cases, were created ranging from PTV conformity weighted to normal tissue sparing weighted. All optimized treatment plans were calculated using three different calculation algorithms (PBC, AAA and MC). In order to study the influence of motion, two virtual lung phantoms were created. The idea was to mimic two different situations: one where the GTV is located centrally in the PTV and another where the GTV was close to the edge of the PTV. PBC is in poor agreement with MC and AAA for all cases and treatment plans. AAA overestimates the dose, compared to MC. This effect is more pronounced for 15 than 6 MV. AAA and MC both predict similar perturbations in dose distributions when moving the GTV to the edge of the PTV. PBC, however, predicts results contradicting those of AAA and MC. This study shows that PB-based dose calculation algorithms are clinically insufficient for patient geometries involving large density inhomogeneities. AAA is in much better agreement with MC, but even a small overestimation of the dose level by the algorithm might lead to a large part of the PTV being underdosed. It is advisable to use low energy as a

  2. Pareto front analysis of 6 and 15 MV dynamic IMRT for lung cancer using pencil beam, AAA and Monte Carlo

    International Nuclear Information System (INIS)

    The pencil beam dose calculation method is frequently used in modern radiation therapy treatment planning regardless of the fact that it is documented inaccurately for cases involving large density variations. The inaccuracies are larger for higher beam energies. As a result, low energy beams are conventionally used for lung treatments. The aim of this study was to analyze the advantages and disadvantages of dynamic IMRT treatment planning for high and low photon energy in order to assess if deviating from the conventional low energy approach could be favorable in some cases. Furthermore, the influence of motion on the dose distribution was investigated. Four non-small cell lung cancer cases were selected for this study. Inverse planning was conducted using Varian Eclipse. A total number of 31 dynamic IMRT plans, distributed amongst the four cases, were created ranging from PTV conformity weighted to normal tissue sparing weighted. All optimized treatment plans were calculated using three different calculation algorithms (PBC, AAA and MC). In order to study the influence of motion, two virtual lung phantoms were created. The idea was to mimic two different situations: one where the GTV is located centrally in the PTV and another where the GTV was close to the edge of the PTV. PBC is in poor agreement with MC and AAA for all cases and treatment plans. AAA overestimates the dose, compared to MC. This effect is more pronounced for 15 than 6 MV. AAA and MC both predict similar perturbations in dose distributions when moving the GTV to the edge of the PTV. PBC, however, predicts results contradicting those of AAA and MC. This study shows that PB-based dose calculation algorithms are clinically insufficient for patient geometries involving large density inhomogeneities. AAA is in much better agreement with MC, but even a small overestimation of the dose level by the algorithm might lead to a large part of the PTV being underdosed. It is advisable to use low energy as a

  3. Development of expressed sequence tag and expressed sequence tag–simple sequence repeat marker resources for Musa acuminata

    OpenAIRE

    Passos, Marco A. N.; de Oliveira Cruz, Viviane; Emediato, Flavia L; de Camargo Teixeira, Cristiane; Souza, Manoel T; Matsumoto, Takashi; Rennó Azevedo, Vânia C.; Ferreira, Claudia F; Amorim, Edson P; de Alencar Figueiredo, Lucio Flavio; Martins, Natalia F; de Jesus Barbosa Cavalcante, Maria; Baurens, Franc-Christophe; da Silva, Orzenil Bonfim; Pappas, Georgios J

    2012-01-01

    Background and aims Banana (Musa acuminata) is a crop contributing to global food security. Many varieties lack resistance to biotic stresses, due to sterility and narrow genetic background. The objective of this study was to develop an expressed sequence tag (EST) database of transcripts expressed during compatible and incompatible banana–Mycosphaerella fijiensis (Mf) interactions. Black leaf streak disease (BLSD), caused by Mf, is a destructive disease of banana. Microsatellite markers were...

  4. Determination of mercury and vanadium concentration in Johnius belangerii (C) fish in Musa estuary in Persian Gulf

    International Nuclear Information System (INIS)

    Highlights: • Hg & V in Johnius belangerii fish in Musa estuary, Persian Gulf was determined. • Significant reverse relationship was between concentrations of Hg & V. • Weight and length had significant direct relationship with Hg concentration. • Length had significant reverse relationship with V concentration. • Concentrations of Hg & V were higher than threshold limits. - Abstract: The main aim of this study was to determine the concentrations of mercury and vanadium in Johnius belangerii (C) fish in the Musa estuary. A total of 67 fishes were caught from the Musa estuary during five intervals of 15 days in the summer of 2013. After biometric measurements were conducted, the concentrations of mercury and vanadium were measured in the muscle tissue of fish using a direct method analyzer (DMA) and a graphite furnace atomic absorption spectrophotometer, respectively. The mean concentration of mercury and vanadium in the muscle tissue of fish was 3.154 ± 1.981 and 2.921 ± 0.873 mg/kg w.w, respectively. The generalized linear model (GLM) analysis showed a significantly positive relationship among mercury concentration, length, and weight (P = 0.000). In addition, there was a significantly negative relationship between vanadium concentration and fish length (P = 0.000). A reverse association was found between concentrations of mercury and vanadium. Mercury concentration exceeded the allowable standards of the Environmental Protection Agency (EPA), the World Health Organization (WHO), and the Food and Drug Administration (FDA) in J. belangerii (C)

  5. Respuesta Glucémica e Insulínica de Pacientes con Diabetes Tipo 2 al consumo de Sopa de Calabaza Criolla (Cucúrbita Pepo L.) Enriquecida con Almidón de Banano Glycemic and Insulinic Response of Patients with Type 2 diabetes to the consumption of Pumpkin Soup Creole (Cucúrbita Pepo L.) Enriched with Banana Starch

    OpenAIRE

    Angel E Torres-Zapata; María A Aparicio-Trápala; Jorge L Blé- Castillo; Carlos A Corzo-Sosa

    2012-01-01

    El objetivo de este trabajo fue determinar el índice glucémico y la respuesta insulínica de una sopa conteniendo almidón nativo de banano. Los efectos benéficos del consumo de almidón resistente sobre el control glucémico han sido reportados previamente, pero estos parámetros no han sido suficientemente estudiados. Las determinaciones se realizaron en 12 sujetos sanos y 12 con diabetes tipo 2 y el índice glucémico de la sopa fue de 31.79. Las áreas bajo las curvas de glucosa e insulina fueron...

  6. Evaluación de la degradación ambiental de materiales termoplásticos empleados en labores agrícolas en el cultivo de banano en Colombia Environmental degradation of thermoplastic materials used in banana agricultural labors in Colombia

    OpenAIRE

    José L. Mejía; Herbert Kerguelén; Angélica Gil; Piedad Gañán

    2007-01-01

    La producción de banano es una dinámica actividad económica en Colombia, sin embargo durante las labores agrícolas, las plantas deben ser unidas unas a otras a través de cuerdas. Esta prácticas contribuye a solventar el peso de los frutos antes de la cosecha. Cuerdas de polipropileno son utilizadas para este fin. Sin embargo, esta práctica genera problemas ambientales en las regiones de cultivo, haciendo necesario identificar materiales biodegradables alternativos. En este estudio, muestras d...

  7. Clinical implications in the use of the PBC algorithm versus the AAA by comparison of different NTCP models/parameters

    International Nuclear Information System (INIS)

    Retrospective analysis of 3D clinical treatment plans to investigate qualitative, possible, clinical consequences of the use of PBC versus AAA. The 3D dose distributions of 80 treatment plans at four different tumour sites, produced using PBC algorithm, were recalculated using AAA and the same number of monitor units provided by PBC and clinically delivered to each patient; the consequences of the difference on the dose-effect relations for normal tissue injury were studied by comparing different NTCP model/parameters extracted from a review of published studies. In this study the AAA dose calculation is considered as benchmark data. The paired Student t-test was used for statistical comparison of all results obtained from the use of the two algorithms. In the prostate plans, the AAA predicted lower NTCP value (NTCPAAA) for the risk of late rectal bleeding for each of the seven combinations of NTCP parameters, the maximum mean decrease was 2.2%. In the head-and-neck treatments, each combination of parameters used for the risk of xerostemia from irradiation of the parotid glands involved lower NTCPAAA, that varied from 12.8% (sd=3.0%) to 57.5% (sd=4.0%), while when the PBC algorithm was used the NTCPPBC’s ranging was from 15.2% (sd=2.7%) to 63.8% (sd=3.8%), according the combination of parameters used; the differences were statistically significant. Also NTCPAAA regarding the risk of radiation pneumonitis in the lung treatments was found to be lower than NTCPPBC for each of the eight sets of NTCP parameters; the maximum mean decrease was 4.5%. A mean increase of 4.3% was found when the NTCPAAA was calculated by the parameters evaluated from dose distribution calculated by a convolution-superposition (CS) algorithm. A markedly different pattern was observed for the risk relating to the development of pneumonitis following breast treatments: the AAA predicted higher NTCP value. The mean NTCPAAA varied from 0.2% (sd = 0.1%) to 2.1% (sd = 0.3%), while the mean NTCPPBC

  8. Foot-and-Mouth Disease Virus 2C Is a Hexameric AAA+ Protein with a Coordinated ATP Hydrolysis Mechanism

    DEFF Research Database (Denmark)

    Sweeney, Trevor; Cisnetto, Valentina; Bose, Daniel;

    2010-01-01

    Foot-and-mouth disease virus (FMDV), a positive sense, single-stranded RNA virus, causes a highly contagious disease in cloven-hoofed livestock. Like other picornaviruses, FMDV has a conserved 2C protein assigned to the superfamily 3 helicases a group of AAA+ ATPases that has a predicted N....... Unliganded 2C(34–318) exhibits concentration-dependent self-association to yield oligomeric forms, the largest of which is tetrameric. Strikingly, in the presence of ATP and RNA, FMDV 2C(34–318) containing the N207A mutation, which binds but does not hydrolyze ATP, was found to oligomerize specifically...... into hexamers. Visualization of FMDV 2C-ATP-RNA complexes by negative stain electron microscopy revealed hexameric ring structures with 6-fold symmetry that are characteristic of AAA+ ATPases. ATPase assays performed by mixing purified active and inactive 2C(34–318) subunits revealed a coordinated mechanism...

  9. Structural Basis for the Magnesium-Dependent Activation and Hexamerization of the Lon AAA+ Protease.

    Science.gov (United States)

    Su, Shih-Chieh; Lin, Chien-Chu; Tai, Hui-Chung; Chang, Mu-Yueh; Ho, Meng-Ru; Babu, C Satheesan; Liao, Jiahn-Haur; Wu, Shih-Hsiung; Chang, Yuan-Chih; Lim, Carmay; Chang, Chung-I

    2016-05-01

    The Lon AAA+ protease (LonA) plays important roles in protein homeostasis and regulation of diverse biological processes. LonA behaves as a homomeric hexamer in the presence of magnesium (Mg(2+)) and performs ATP-dependent proteolysis. However, it is also found that LonA can carry out Mg(2+)-dependent degradation of unfolded protein substrate in an ATP-independent manner. Here we show that in the presence of Mg(2+) LonA forms a non-secluded hexameric barrel with prominent openings, which explains why Mg(2+)-activated LonA can operate as a diffusion-based chambered protease to degrade unstructured protein and peptide substrates efficiently in the absence of ATP. A 1.85 Å crystal structure of Mg(2+)-activated protease domain reveals Mg(2+)-dependent remodeling of a substrate-binding loop and a potential metal-binding site near the Ser-Lys catalytic dyad, supported by biophysical binding assays and molecular dynamics simulations. Together, these findings reveal the specific roles of Mg(2+) in the molecular assembly and activation of LonA. PMID:27041593

  10. AAA-ATPase FIDGETIN-LIKE 1 and Helicase FANCM Antagonize Meiotic Crossovers by Distinct Mechanisms.

    Directory of Open Access Journals (Sweden)

    Chloe Girard

    2015-07-01

    Full Text Available Meiotic crossovers (COs generate genetic diversity and are critical for the correct completion of meiosis in most species. Their occurrence is tightly constrained but the mechanisms underlying this limitation remain poorly understood. Here we identified the conserved AAA-ATPase FIDGETIN-LIKE-1 (FIGL1 as a negative regulator of meiotic CO formation. We show that Arabidopsis FIGL1 limits CO formation genome-wide, that FIGL1 controls dynamics of the two conserved recombinases DMC1 and RAD51 and that FIGL1 hinders the interaction between homologous chromosomes, suggesting that FIGL1 counteracts DMC1/RAD51-mediated inter-homologue strand invasion to limit CO formation. Further, depleting both FIGL1 and the previously identified anti-CO helicase FANCM synergistically increases crossover frequency. Additionally, we showed that the effect of mutating FANCM on recombination is much lower in F1 hybrids contrasting from the phenotype of inbred lines, while figl1 mutation equally increases crossovers in both contexts. This shows that the modes of action of FIGL1 and FANCM are differently affected by genomic contexts. We propose that FIGL1 and FANCM represent two successive barriers to CO formation, one limiting strand invasion, the other disassembling D-loops to promote SDSA, which when both lifted, leads to a large increase of crossovers, without impairing meiotic progression.

  11. Avaliação de genótipos de bananeira à Colletotrichum musae em pós-colheita Evaluation of genotypes of banana to Colletotrichum musae in postharvest

    Directory of Open Access Journals (Sweden)

    Danilo Batista Pinho

    2010-09-01

    Full Text Available Alguns hibridos tetraploides apresentam resistência à antracnose, porém são reduzidas as informações sobre os níveis de resistência desses genótipos e o comportamento dos mesmos no Brasil. O objetivo do presente trabalho foi avaliar a incidência e a severidade de antracnose sobre o comportamento de diferentes genótipos de bananeiras resistentes a Sigatoka-Negra em função de diferentes concentrações de conídios de Colletotrichum musae em diferentes épocas de avaliação. Os frutos colhidos no estádio de maturação fisiológica dos genótipos, Thap Maeo, Caipira, Pacovan Ken, Ambrosia, PV 42-53, PA 42-44, FHIA 01, FHIA 18 e a cultivar Prata-Anã utilizada como testemunha. Buquês contendo três frutos foram atomizados com diferentes concentrações (0; 10²; 10³; 10(4; 10(5 e 10(6 conídios/mL de C. musae. Os frutos foram incubados a 25 ºC. A incidência e severidade da antracnose foi avaliada aos 3; 6; 9 e 12 dias após a inoculação. Foi utilizado o delineamento inteiramente casualizado, em esquema fatorial 9x6x4 (genótipos, concentrações de esporos e épocas de avaliação, com quatro repetições, contendo quatro buquês de três frutos. Aos doze dias de avaliação, todos os frutos de todos os genótipos estudados apresentaram incidência de C. musae quando inoculados com as diferentes concentrações de inóculo. A cultivar Thap Maeo apresentou a menor severidade (27,5% de antracnose, enquanto os genótipos Ambrosia, PV 42-53, FHIA 18 e FHIA 01 foram as mais suscetíveis à antracnose com 64%, 64%, 61,33% e 58% de severidade, respectivamente.Some tetraploides hybrids present resistance to anthracnose, however the information on the levels of resistance of these genotypes and the behavior in Brazil are reduced. The aim of this study was to evaluate the incidence and severity of injury on the behavior of different banana genotypes resistant to Black Sigatoka for different concentrations of conidia of Colletotrichum

  12. MusA: using indoor positioning and navigation to enhance cultural experiences in a museum.

    Science.gov (United States)

    Rubino, Irene; Xhembulla, Jetmir; Martina, Andrea; Bottino, Andrea; Malnati, Giovanni

    2013-01-01

    In recent years there has been a growing interest in the use of multimedia mobile guides in museum environments. Mobile devices have the capabilities to detect the user context and to provide pieces of information suitable to help visitors discover and follow the logical and emotional connections that develop during the visit. In this scenario, location based services (LBS) currently represent an asset, and the choice of the technology to determine users' position, combined with the definition of methods that can effectively convey information, become key issues in the design process. In this work, we present Museum Assistant (MusA), a general framework for the development of multimedia interactive guides for mobile devices. Its main feature is a vision-based indoor positioning system that allows the provision of several LBS, from way-finding to the contextualized communication of cultural contents, aimed at providing a meaningful exploration of exhibits according to visitors' personal interest and curiosity. Starting from the thorough description of the system architecture, the article presents the implementation of two mobile guides, developed to respectively address adults and children, and discusses the evaluation of the user experience and the visitors' appreciation of these applications. PMID:24351645

  13. MusA: Using Indoor Positioning and Navigation to Enhance Cultural Experiences in a Museum

    Directory of Open Access Journals (Sweden)

    Irene Rubino

    2013-12-01

    Full Text Available In recent years there has been a growing interest in the use of multimedia mobile guides in museum environments. Mobile devices have the capabilities to detect the user context and to provide pieces of information suitable to help visitors discover and follow the logical and emotional connections that develop during the visit. In this scenario, location based services (LBS currently represent an asset, and the choice of the technology to determine users’ position, combined with the definition of methods that can effectively convey information, become key issues in the design process. In this work, we present Museum Assistant (MusA, a general framework for the development of multimedia interactive guides for mobile devices. Its main feature is a vision-based indoor positioning system that allows the provision of several LBS, from way-finding to the contextualized communication of cultural contents, aimed at providing a meaningful exploration of exhibits according to visitors’ personal interest and curiosity. Starting from the thorough description of the system architecture, the article presents the implementation of two mobile guides, developed to respectively address adults and children, and discusses the evaluation of the user experience and the visitors’ appreciation of these applications.

  14. Improvement of banana (Musa cvs.) through in vitro culture techniques and induced mutations

    International Nuclear Information System (INIS)

    Rapidly multiplying shoot tip cultures were established from meristem explants of banana (Musa cvs.). Banana cultivars were successfully propagated on modified Murashige and Skoog medium with 4.5 mg/l BAP. Roots were induced on plantlets cultured on 1/2 strength MS medium containing 0.20 mg/l IBA. Plantlets were acclimatized and transferred to soil for further evaluation. Proembryogenic calli were initiated from basal leaf sheaths and rhizome tissue cultured on SH medium with 6.63 mg/l dicamba. Protoplasts were isolated form rhizome tissue using an enzyme solution containing 3% cellulase R-10, 1% macerozyme R-10 and 1% pectinase. Efforts are in progress to regenerate plants via somatic embryogenesis and from isolated protoplasts. A mutation induction experiment was carried out by irradiating vegetative shoot apices at 5, 10, 15 and 20 Gy using a 60Co source. Radiosensitivity was assessed by determining the subsequent rate of shoot differentiation. (author). 19 refs, 1 fig., 1 tab

  15. Molecular Characterisation of Endophytic Fungi from Roots of Wild Banana (Musa acuminata).

    Science.gov (United States)

    Zakaria, Latiffah; Jamil, Muhamad Izham Muhamad; Anuar, Intan Sakinah Mohd

    2016-02-01

    Endophytic fungi inhabit apparently healthy plant tissues and are prevalent in terrestrial plants, especially root tissues, which harbour a wide assemblage of fungal endophytes. Therefore, this study focused on the isolation and characterisation of endophytic fungi from the roots of wild banana (Musa acuminata). A total of 31 isolates of endophytic fungi were isolated from 80 root fragments. The endophytic fungi were initially sorted according to morphological characteristics and identified using the sequences of the translation elongation factor-1α (TEF-1α) gene of Fusarium spp. and the Internal Transcribed Spacer (ITS) regions of other fungi. The most common fungal isolates were species of the genus Fusarium, which were identified as F. proliferatum, Fusarium sp., F. solani species complex, and F. oxysporum. Other isolated endophytic fungi included Curvularia lunata, Trichoderma atroviride, Calonectria gracilis, Rhizoctonia solani, Bionectria ochroleuca, and Stromatoneurospora phoenix (Xylariceae). Several of the fungal genera, such as Fusarium, Trichoderma, Rhizoctonia, and Xylariceae, are among the common fungal endophytes reported in plants. This study showed that the roots of wild banana harbour a diverse group of endophytic fungi. PMID:27019688

  16. Effect of Musa spp. extract on eggs and larvae of gastrointestinal nematodes from infected sheep

    Directory of Open Access Journals (Sweden)

    Natalie Neuwirt

    2015-12-01

    Full Text Available Helminthes are listed as one of the main problems facing the development of goat and sheep production. Haemonchus contortus is the specie that causes greatest negative impact in ranching. Resistance to anti-parasitic drugs and demand for residue-free animal-derived food products has elevated the importance of herbal treatments. The aim of this study was to develop an extract of Musa spp. and assess by in vitro testing, the anthelmintic effect on eggs and larvae in the gastrointestinal nematodes in sheep. Stool samples from sheep naturally infected were used to obtain eggs and larvae and was then followed by a test of hatchability and a larval migration inhibition test. In vitro tests on the inhibition of larval hatchability at concentrations of 160 and 180 mg mL-1 of larval extracts and inhibition of migration at concentrations of 800 and 1000 mg mL-1 were observed. The results indicate that the use of banana leaf has an anthelmintic effect and that in vivo studies on the applicability of this technology to the field should be made to further understanding and bring more information to what has already been revealed in this study.

  17. Isolation and Characterization of an α-Glucosidase Inhibitor from Musa spp. (Baxijiao Flowers

    Directory of Open Access Journals (Sweden)

    Zhanwu Sheng

    2014-07-01

    Full Text Available The use of α-glucosidase inhibitors is considered to be an effective strategy in the treatment of diabetes. Using a bioassay-guided fractionation technique, five Bacillus stearothermophilus α-glucosidase inhibitors were isolated from the flowers of Musa spp. (Baxijiao. Using NMR spectroscopy analysis they were identified as vanillic acid (1, ferulic acid (2, β-sitosterol (3, daucosterol (4 and 9-(4′-hydroxyphenyl-2-methoxyphenalen-1-one (5. The half maximal inhibitory concentration (IC50 values of compounds 1–5 were 2004.58, 1258.35, 283.67, 247.35 and 3.86 mg/L, respectively. Compared to a known α-glucosidase inhibitor (acarbose, IC50 = 999.31 mg/L, compounds 3, 4 and 5 showed a strong α-glucosidase inhibitory effect. A Lineweaver-Burk plot indicated that compound 5 is a mixed-competitive inhibitor, while compounds 3 and 4 are competitive inhibitors. The inhibition constants (Ki of compounds 3, 4 and 5 were 20.09, 2.34 and 4.40 mg/L, respectively. Taken together, these data show that the compounds 3, 4 and 5 are potent α-glucosidase inhibitors.

  18. Musa paradisica peel extract as green corrosion inhibitor for mild steel in HCl solution

    International Nuclear Information System (INIS)

    Highlights: • Banana peels have been tested for corrosion inhibition of mild steel in 1 M HCl. • Weight loss, electrochemical and AFM Techniques are used for inhibition study. • Maximum corrosion inhibition of 92% is achieved using raw banana peel extract. • The extracts have been characterized using HPLC, UV–vis, and FTIR techniques. • Gallocatechin and catechin is responsible for inhibitive property of the extracts. - Abstract: The inhibition and adsorption effects of the aqueous extracts of Musa paradisica (Banana) peels on mild steel corrosion in 1 M HCl as well as change in inhibition efficiency with ripening of the peels are investigated by weight loss measurement, electrochemical impedance spectroscopy (EIS), Tafel polarization and atomic force microscopy (AFM) techniques. Inhibition ability of the extracts decreases with the maturity stages. Furthermore, the extracts are characterized by FTIR spectroscopy, UV–visible spectroscopy and high performance liquid chromatography (HPLC) techniques. Adsorption behavior of the extracts is also studied, which suggest Langmuir isotherm model as a most suitable adsorption mechanism

  19. CINÉTICA ENZIMÁTICA DE LA POLIFENOL OXIDASA DEL BANANO GROS MICHEL EN DIFERENTES ESTADOS DE MADURACIÓN ENZYME KINETICS OF POLYPHENOL OXIDASE FROM GROS MICHEL BANANA THROUGH DIFFERENT MATURATION STAGES

    Directory of Open Access Journals (Sweden)

    Claudia L. GARCÍA W.

    2006-01-01

    Full Text Available La postcosecha del banano se acompaña de múltiples reacciones enzimáticas, causantes de la degradación de almidones y clorofila, de la síntesis de azúcares y carotenos, y de los cambios en la acidez, ablandamiento de tejidos y pardeamiento enzimático. Este pardeamiento es causado por la acción de la Polifenol Oxidasa (PFO sobre los fenoles contenidos en el fruto y es considerado un cambio indeseable por el consumidor. Con el objeto de determinar el momento de mínima actividad enzimática para lograr su óptimo procesamiento, se caracterizó la variedad Gros Michel en diferentes estados de maduración, en cuanto a su color, textura, actividad de agua, humedad y grados Brix, y luego se midió la cinética enzimática, aprovechando la formación del color café durante el pardeamiento, empleando un espectrofotómetro de UV-visible a 420nm. También se cuantificó la cantidad de proteína (enzima mediante el método de Bradford (lectura a 595nm. Una vez identificadas las cinéticas de cada estado de maduración, se hizo una comparación entre cada uno de los grupos mediante la técnica estadística de Análisis de Varianza con Medidas Repetidas en el tiempo, que mostró una marcada diferencia, estadísticamente significativa, entre los bananos de menor y mayor estado de maduración en forma decreciente, y se encontró el punto mínimo de actividad de la enzima en los estados más avanzados de maduración.The post harvest of banana is accompanied by multiple enzymatic reactions, which cause the degradation of starches and chlorophyll, the synthesis of sugars and carotenes, changes in the acidity, tissue softening and enzymatic browning. This browning is caused by the action of the Polyphenoloxidase (PPO on the phenols contained in the fruit and is considered an undesirable change by the consumer. To determine the time of minimum enzymatic activity in order to obtain its optimal processing, the variety Gros Michel was characterized in colour

  20. Micromicetos del suelo de una plantación de plátano (Musa paradisiaca) en Teapa, Tabasco, México Soil microfungi from a banana (Musa paradisiaca) plantation in Teapa, Tabasco, Mexico

    OpenAIRE

    Mariana Del Olmo-Ruiz; Joaquín Cifuentes-Blanco; Guadalupe Vidal-Gaona; Edmundo Rosique-Gil

    2010-01-01

    Se analizó la micobiota presente en el suelo de un cultivo de plátano (Musa paradisiaca L.) en el municipio de Teapa en el estado de Tabasco, México. Los objetivos fueron conocer las especies habitantes de este suelo y la dinámica de la comunidad con respecto a la composición de especies a lo largo de un año. Se llevaron a cabo 4 muestreos durante temporadas distintas y los aislamientos se realizaron mediante la técnica de dilución en placa. Se recuperaron 91 colonias, correspondientes a 30 e...

  1. Ensayo de toxicidad aguda oral de un fitofármaco obtenido a partir del pseudotallo de Musa paradisiaca L.

    OpenAIRE

    Guevara Orellanes, Irania; Rodriguez Torres, Caridad C.; Pérez Capote, María Regla; León Fernández, Olga Sonia; Rodriguez, Susana Sam; Álvarez, Dalia; Castañeda, Juana; Vega, Jorge Luis

    2003-01-01

    El fitofármaco elaborado a partir del extracto de pseudotallo de Musa paradisiaca es un producto natural que presenta además en su composición miel de abejas (edulcorante) y propóleos (preservante). Entre los requisitos para el registro de medicamentos se encuentran los ensayos toxicológicos, los cuales garantizan un margen de seguridad para el empleo de los productos que son evaluados, según las normativas establecidas por la OECD. Para el estudio de toxicidad aguda oral se emplearon rata...

  2. Effet des différentes balances hormonales sur la microprogation du Cv. Hybride FHIA-01 (Musa sp.)

    OpenAIRE

    Mazinga, KM.; Van Koninckxloo, M.; Godoy Jara, M.; Baboy Longanza, L.

    2013-01-01

    Effects of Different Hormonal Balances on the Microprogation of Cv. Hybrid FHIA-01 (Musa sp.). Different hormonal balances were evaluated to develop a growth medium to effectively multiply the FHIA-01 hybrid in vitro The most striking results of this study are: the almost complete inhibition of the shoot proliferation by BAP; the total root inhibition by the concentration 10 μM BAP+10 μM MemTR and the improvement of the mean shoot proliferation (5,4 shoots/explants) by adding the same concent...

  3. Penggunaan Daun Pisang Batu (Musa Balbisiana Colla) Sebagai Adsorben Untuk Menyerap Logam Crom (Cr) Dan Nikel (Ni)

    OpenAIRE

    Hanim, Ulfa

    2011-01-01

    A research for the using of batu banana leaves (Musa Balbisiana Colla) as adsorbent of chrome (Cr) and nickel (Ni), has been done. The sample of dried batu banana leaves that had be powdered in the size of 50 mesh and mixed with NaOH 20% activated by using HCl 1 N solution. In this research, the pH is measured on the chrome (Cr) and nickel (Ni) using pH meter and the measurement of chrome (Cr) and nickel (Ni) content in the sample of dried batu banana leaves before activation and after activa...

  4. Annotation of Differentially Expressed Genes in the Somatic Embryogenesis of Musa and Their Location in the Banana Genome

    OpenAIRE

    Josefina Ines Maldonado-Borges; José Roberto Ku-Cauich; Rosa Maria Escobedo-GraciaMedrano

    2013-01-01

    Analysis of cDNA-AFLP was used to study the genes expressed in zygotic and somatic embryogenesis of Musa acuminata Colla ssp. malaccensis, and a comparison was made between their differential transcribed fragments (TDFs) and the sequenced genome of the double haploid- (DH-) Pahang of the malaccensis subspecies that is available in the network. A total of 253 transcript-derived fragments (TDFs) were detected with apparent size of 100–4000 bp using 5 pairs of AFLP primers, of which 21 were di...

  5. Analysis of expressed sequence tags from Musa acuminata ssp. burmannicoides, var. Calcutta 4 (AA) leaves submitted to temperature stresses

    OpenAIRE

    Santos, C R; Martins, N.F.; Horberg, H.M.; Almeida, E.R.P.; Coelho, M.C.F.; Togawa, R.; F. R. Silva; Caetano, A.R.; Miller, R.N.G.; de Souza, M T

    2005-01-01

    In order to discover genes expressed in leaves of Musa acuminata ssp. burmannicoides var. Calcutta 4 (AA), from plants submitted to temperature stress, we produced and characterized two full-length enriched cDNA libraries. Total RNA from plants subjected to temperatures ranging from 5°C to 25°C and from 25°C to 45°C was used to produce a COLD and a HOT cDNA library, respectively. We sequenced 1,440 clones from each library. Following quality analysis and vector trimming, we assembled 2,286 se...

  6. Fusarium species associated with fruit rot of banana (Musa spp.), papaya (Carica papaya) and guava (Psidium guajava)

    OpenAIRE

    Zakaria, L.; Mazzura, W. C.; Kong, W. H.; S. Baharuddin

    2012-01-01

    A total of 60 isolates of Fusarium were isolated from fruit rot of banana (Musa spp.), papaya (Carica papaya) and guava(Psidium guajava). The most common species recovered from the fruit rot of the three fruit crops were F. semitectum(40 %), F. solani (38.3 %), F. verticillioides (11.7 %) and F. oxysporum (10 %). Fusarium semitectum was isolated from fruit rot of banana, papaya and guava; F. oxysporum from banana and papaya; F. solani from banana and guava and F.verticillioides from banana. F...

  7. Verification of Rapid Arc™ planning with AAA algorithm using an inhomogeneous 3D phantom

    International Nuclear Information System (INIS)

    New technologies have been developed to improve the quality assurance of the planning with modulated beams. One way to deal with the high costs of the dosimetry equipment was to develop a 3D phantom, using TLDs and radiochromic film, designed by the Radiotherapy Quality Program of INCa. The calculus was done using the AAA algorithm with heterogeneity correction, making the phantom rather heterogeneous. Five measurements related to the Rapid Arc™ planning were taken, once there was the phantom CT for optimization. The purpose of this work is a 3D verification of the dose distribution in the heterogeneous phantom. The mean deviation in planning target volumes was lower than ±5%. On the other side, the results dispersion for the others heterogeneities was higher, the maximum mean deviation obtained, for example, for the heterogeneity related to the bladder, was 7.41%. The maximum standard deviation found for both cases was around 9% for the target heterogeneity and 11% for the other heterogeneities. The phantom might be an interesting tool in order to verify the Rapid Arc™ planning, however, more statistical data is necessary as to achieve better results for the analysis of dose distribution. (author)heterogeneous phantom. The mean deviation in planning target volumes was lower than ±5%. On the other side, the results dispersion for the others heterogeneities was higher, the maximum mean deviation obtained, for example, for the heterogeneity related to the bladder, was 7.41%. The maximum standard deviation found for both cases was around 9% for the target heterogeneity and 11% for the other heterogeneities. The phantom might be an interesting tool in order to verify the Rapid Arc™ planning, however, more statistical data is necessary as to achieve better results for the analysis of dose distribution. (author)

  8. A proteomic study of Corynebacterium glutamicum AAA+ protease FtsH

    Directory of Open Access Journals (Sweden)

    Schluesener Daniela

    2007-01-01

    Full Text Available Abstract Background The influence of the membrane-bound AAA+ protease FtsH on membrane and cytoplasmic proteins of Corynebacterium glutamicum was investigated in this study. For the analysis of the membrane fraction, anion exchange chromatography was combined with SDS-PAGE, while the cytoplasmic protein fraction was studied by conventional two-dimensional gel electrophoresis. Results In contrast to the situation in other bacteria, deletion of C. glutamicum ftsH has no significant effect on growth in standard minimal medium or response to heat or osmotic stress. On the proteome level, deletion of the ftsH gene resulted in a strong increase of ten cytoplasmic and membrane proteins, namely biotin carboxylase/biotin carboxyl carrier protein (accBC, glyceraldehyde-3-phosphate dehydrogenase (gap, homocysteine methyltransferase (metE, malate synthase (aceB, isocitrate lyase (aceA, a conserved hypothetical protein (NCgl1985, succinate dehydrogenase A (sdhA, succinate dehydrogenase B (sdhB, succinate dehydrogenase CD (sdhCD, and glutamate binding protein (gluB, while 38 cytoplasmic and membrane-associated proteins showed a decreased abundance. The decreasing amount of succinate dehydrogenase A (sdhA in the cytoplasmic fraction of the ftsH mutant compared to the wild type and its increasing abundance in the membrane fraction indicates that FtsH might be involved in the cleavage of a membrane anchor of this membrane-associated protein and by this changes its localization. Conclusion The data obtained hint to an involvement of C. glutamicum FtsH protease mainly in regulation of energy and carbon metabolism, while the protease is not involved in stress response, as found in other bacteria.

  9. Fructans and other water soluble carbohydrates in vegetative organs and fruits of different Musa spp. accessions.

    Science.gov (United States)

    Cruz-Cárdenas, Carlos I; Miranda-Ham, María L; Castro-Concha, Lizbeth A; Ku-Cauich, José R; Vergauwen, Rudy; Reijnders, Timmy; Van den Ende, Wim; Escobedo-GraciaMedrano, Rosa M

    2015-01-01

    The water soluble carbohydrates (WSC) glucose, fructose, and sucrose are well-known to the great public, but fructans represent another type of WSC that deserves more attention given their prebiotic and immunomodulatory properties in the food context. Although the occurrence of inulin-type fructo-oligosaccharides (FOS) was proposed in the fruit of some banana accessions, little or no information is available neither on the exact identity of the fructan species, nor on the fructan content in different parts of banana plants and among a broader array of banana cultivars. Here, we investigated the WSC composition in leaves, pulp of ripe fruits and rhizomes from mature banana plants of 11 accessions (I to XI), including both cultivated varieties and wild Musa species. High performance anion exchange chromatography with integrated pulsed amperometric detection (HPAEC-IPAD) showed the presence of 1-kestotriose [GF2], inulobiose [F2], inulotriose [F3], 6-kestotriose and 6G-kestotriose (neokestose) fructan species in the pulp of mature fruits of different accessions, but the absence of 1,1-nystose and 1,1,1 kestopentaose and higher degree of polymerization (DP) inulin-type fructans. This fructan fingerprint points at the presence of one or more invertases that are able to use fructose and sucrose as alternative acceptor substrates. Quantification of glucose, fructose, sucrose and 1-kestotriose and principal component analysis (PCA) identified related banana groups, based on their specific WSC profiles. These data provide new insights in the biochemical diversity of wild and cultivated bananas, and shed light on potential roles that fructans may fulfill across species, during plant development and adaptation to changing environments. Furthermore, the promiscuous behavior of banana fruit invertases (sucrose and fructose as acceptor substrates besides water) provides a new avenue to boost future work on structure-function relationships on these enzymes, potentially leading to

  10. Fructans and other water soluble carbohydrates in vegetative organs and fruits of different Musa spp. accessions

    Directory of Open Access Journals (Sweden)

    Carlos Ivan eCruz Cardenas

    2015-06-01

    Full Text Available The water-soluble carbohydrates (WSC glucose, fructose and sucrose are well-known to the great public, but fructan represents another type of WSC that deserves more attention given their prebiotic and immunomodulatory properties in the food context. Although the occurrence of inulin-type fructo-oligosaccharides (FOS was proposed in the fruit of some banana accessions, little or no information is available neither on the exact identity of the fructan species, nor on the fructan content in different parts of banana plants and among a broader array of banana cultivars. Here, we investigated the WSC composition in leaves, pulp of ripe fruits and rhizomes from mature banana plants of eleven accessions (I to XI, including both cultivated varieties and wild Musa species. High performance anion exchange chromatography with integrated pulsed amperometric detection (HPAEC-IPAD showed the presence of 1-kestotriose [GF2], inulobiose [F2], inulotriose [F3], 6-kestotriose and 6G-kestotriose (neokestose fructan species in the pulp of mature fruits of different accessions, but the absence of 1,1-nystose and 1,1,1 kestopentaose and higher degree of polymerization (DP inulin-type fructans. This fructan fingerprint points at the presence of one or more invertases that are able to use fructose and sucrose as alternative acceptor substrates. Quantification of glucose, fructose, sucrose and 1-kestotriose and principal component analysis (PCA identified related banana groups, based on their specific WSC profiles. These data provide new insights in the biochemical diversity of wild and cultivated bananas, and shed light on potential roles that fructans may fulfil across species, during plant development and adaptation to changing environments. Furthermore, the promiscuous behavior of banana fruit invertases (sucrose and fructose as acceptor substrates besides water provides a new avenue to boost future work on structure-function relationships on these enzymes

  11. Arylsulphatase in the rhizosphere of plantain Musa AAB and its relation with growth, development and pr Arilsulfatasa en la rizosfera de plátano, Musa AAB y relación con crecimiento, desarrollo y produccion

    Directory of Open Access Journals (Sweden)

    Rao Idupulapati M

    2006-09-01

    Full Text Available

    Arylsulphatase in the rhizosphere of plantain Musa AAB and its relation with growth, development and production. In a plantain crop (Musa AAB of the Central coffee growing zone of Colombia, arylsulphatase activity in a Pachic Melanudand Medial Isotermic soil under three agronomic management systems during 18 months was measured. Measurements were done at 6, 12 and 18 months in four depths, 0-5, 5-10, 10-20, 20-30 cm. The highest arylsulphatase activity (60% was found in the first 10 cm, with a decreasing in depth. Statical analysis did not show significant differences among the three plant ages. Arylsulphatase activity was found between 164 µmol g-1 min-1 and 319 µmol g-1 min-1. The lowest activity was found in the rhizosphere of plants with 12 months of age (flowering under chemical management. Arylsulphatase activity showed very significant correlation with Biomass-C and with Mg in soil, number of leaf and the plantain bunch weight.

    Key words: Enzymic activity-arylsulphatase, andisol, Musa ABB

    En la zona central cafetera de Colombia, en un suelo Pachic Melanudands medial isotérmico, se midió la actividad de arilsulfatasa en rizosfera de plátano Musa AAB, en tres sistemas de manejo agronómico (químico o convencional, tradicional y ecológico, cuatro profundidades (0-5, 5-10, 10-20 y 20-30 cm y tres edades de cultivo (6 meses – diferenciación floral, 12 meses – floración, 18 meses - cosecha. La mayor actividad (60% se encontró en los primeros 10 cm., y disminuyó a medida que se profundizó en el perfil del suelo. El análisis estadístico no mostró diferencias significativas entre los tres manejos o entre edades de planta. La actividad de arilsulfatasa se encontró entre 164 µ mol g-1 min-1 y 319 µ

  12. Alejandro García Reidy, Las musas rameras. Oficio dramático y conciencia profesional en Lope de Vega

    Directory of Open Access Journals (Sweden)

    Antonio Carreño

    2015-01-01

    Full Text Available Review of Alejandro García Reidy, Las musas rameras. Oficio dramático y conciencia profesional en Lope de Vega, Iberoamericana / Vervuert (Colección escena Clásica, 2, Madrid / Frankfurt am Main, 2013, 440 pp. ISBN: 9788484897439.

  13. MuSAE: A European Project for the Diffusion of Energy and Environmental Planning in Small-Medium Sized Municipalities

    Directory of Open Access Journals (Sweden)

    Giorgio Baldinelli

    2015-12-01

    Full Text Available The basic idea of the EU LIFE+ 2011 project MuSAE (“Municipalities Subsidiarity for Actions on Energy”, code LIFE11 ENV/IT/000016 consists of transferring the skills and experience related to energy planning, acquired by the leading beneficiary, the Municipality of Perugia, to three small- or medium-sized Umbrian Municipalities (Marsciano, Umbertide and Lisciano Niccone. This transfer is aimed, among other objectives, at the drafting of the Municipal Energy and Environmental Plan (MEEP and the opening of an energy information office in each partner Municipality, in cooperation with CIRIAF and Umbria Region. The present paper provides a summary of MuSAE activities, analyzing the procedures and modalities of implementation of the various phases of the MEEPs, on the basis of the experience gained over the years through the collaboration with the Municipality of Perugia and adapted to smaller territories such as those represented by the other partner Municipalities. A summary of the dissemination activities and pilot projects is also presented, testifying the first concrete results of the planning activity developed by each administration within the project.

  14. Hypolipidemic Effects of Aqueous Extract of Three Cultivars of Musa sapientum Fruit Peel on Poloxamer-407 Induced Hyperlipidemic Wistar Rats.

    Directory of Open Access Journals (Sweden)

    C EDENTA

    2014-12-01

    Full Text Available Objective: The effect of aqueous extracts of the ripped fruit peel of three cultivars of Musa sapientum (Saro, Ominni and Oranta on the lipid profile of normolipidemic and hyperlipidemic rats were examined. Methods: Aqueous peel extracts of the 3-cultivars of Musa sapientum (100mg/Kg bw were administered to normolipidemic and poloxamer-407 induced hyperlidemic rats (140-180g. Atorvastatin was used as standard drug (70 mg/Kg bw. Blood samples were collected for determination of plasma total cholesterol (Tc, triacylglycerides (TAG, low-density lipoprotein cholesterol (LDL-c and high-density lipoprotein cholesterol (HDL-c after 21-days in normolipidemic rats and 3-days in hyperlipidemic rats. Results: Normolipidemic rats treated with extract of Saro showed a significant (p0.05 difference when compared to the Atorvastatin and normal control groups. Conclusion: The findings in this study reveals Oranta cultivar of M. sapientum as a better potential drug candidate among the cultivars studied, hence could be useful for the treatment of hyperlipidemia and other cardiovascular related diseases.

  15. Production of Haploid Tissues and SNP Analysis of the Genome in Musa acuminata cv.'Matti'(AA)

    International Nuclear Information System (INIS)

    Haploid and doubled haploid plants are of considerable value in genetic studies, genomics and plant breeding, allowing characterization and exploitation of genes where only one allele is present. Inbred banana lines have not been developed and with very few haploids or homozygotes reported, we aimed to develop new materials for genetic and genomic studies and to see if protocols could be improved using a range of genetic material not previously investigated. Anther culture was carried out using four Musa acuminata (AA) cultivars grown in homestead cultivation in Kerala, southern India. Protocols based on using modified MS medium supplemented with 2,4-D and BA (0.1 mg 1-1 each) were used. Embryogenic callus was regenerated from 4% of anthers of cv. Matti. The callus produced somatic embryosthat developed small leaves but haploid plant regeneration was not achieved. Regenerated leaf tissues had a haploid set of chromosome complements. The haploid tissuesprovided a source of DNA which may be suitable for whole genome shotgun approaches to re-sequencing, where the high levels of heterozygosity in Musa would impair assembly. (author)

  16. Analysis of expressed sequence tags from Musa acuminata ssp. burmannicoides, var. Calcutta 4 (AA) leaves submitted to temperature stresses.

    Science.gov (United States)

    Santos, C M R; Martins, N F; Hörberg, H M; de Almeida, E R P; Coelho, M C F; Togawa, R C; da Silva, F R; Caetano, A R; Miller, R N G; Souza, M T

    2005-05-01

    In order to discover genes expressed in leaves of Musa acuminata ssp. burmannicoides var. Calcutta 4 (AA), from plants submitted to temperature stress, we produced and characterized two full-length enriched cDNA libraries. Total RNA from plants subjected to temperatures ranging from 5 degrees C to 25 degrees C and from 25 degrees C to 45 degrees C was used to produce a COLD and a HOT cDNA library, respectively. We sequenced 1,440 clones from each library. Following quality analysis and vector trimming, we assembled 2,286 sequences from both libraries into 1,019 putative transcripts, consisting of 217 clusters and 802 singletons, which we denoted Musa acuminata assembled expressed sequence tagged (EST) sequences (MaAES). Of these MaAES, 22.87% showed no matches with existing sequences in public databases. A global analysis of the MaAES data set indicated that 10% of the sequenced cDNAs are present in both cDNA libraries, while 42% and 48% are present only in the COLD or in the HOT libraries, respectively. Annotation of the MaAES data set categorized them into 22 functional classes. Of the 2,286 high-quality sequences, 715 (31.28%) originated from full-length cDNA clones and resulted in a set of 149 genes. PMID:15841358

  17. Genotype-by-Environment Interaction and Testing Environments for Plantain and Banana (Musa spp. L. Breeding in West Africa

    Directory of Open Access Journals (Sweden)

    Ortiz, R.

    1999-01-01

    Full Text Available With reduced budgets allocated for international agricultural research, site rationalisation had become an important issue to consider when carrying out multilocational testing of promising selections. The aim of this paper was to determine the importance of the genotype-by-environment interaction in multilocational trials of plantains and bananas (Musa spp. L. in selected sites of West Africa comprising the humid forest and the forest-savanna transition zones. A sample of plantain-banana hybrids, plantain landraces, exotic banana cultivars and diploid parental banana accessions were evaluated in three locations : Mbalmayo and Onne (humid forest and Ibadan (forest-savanna transition. The experimental results of our research suggested that multilocational testing is more profitable than single site evaluation over several years in the Musa breeding station. Furthermore, based on correlated responses across environments for yield potential, we suggest that one of the selection sites in the humid forest (i. e., Mbalmayo be dropped since selections in one site (Onne may be well adapted to the other location in the same agroecozone. Conversely, the relatively poor performance of most genotypes in dry environments (e. g. Ibadan reinforces the importance of early testing across a wide range of environments. In this way selections with broad or specific adaptation may be identified for further release to targeted farmers.

  18. A dosimetric evaluation of the Eclipse AAA algorithm and Millennium 120 MLC for cranial intensity-modulated radiosurgery

    Energy Technology Data Exchange (ETDEWEB)

    Calvo Ortega, Juan Francisco, E-mail: jfcdrr@yahoo.es; Moragues, Sandra; Pozo, Miquel; José, Sol San; Puertas, Enrique; Fernández, Jaime; Casals, Joan

    2014-07-01

    The aim of this study is to assess the accuracy of a convolution-based algorithm (anisotropic analytical algorithm [AAA]) implemented in the Eclipse planning system for intensity-modulated radiosurgery (IMRS) planning of small cranial targets by using a 5-mm leaf-width multileaf collimator (MLC). Overall, 24 patient-based IMRS plans for cranial lesions of variable size (0.3 to 15.1 cc) were planned (Eclipse, AAA, version 10.0.28) using fixed field-based IMRS produced by a Varian linear accelerator equipped with a 120 MLC (5-mm width on central leaves). Plan accuracy was evaluated according to phantom-based measurements performed with radiochromic film (EBT2, ISP, Wayne, NJ). Film 2D dose distributions were performed with the FilmQA Pro software (version 2011, Ashland, OH) by using the triple-channel dosimetry method. Comparison between computed and measured 2D dose distributions was performed using the gamma method (3%/1 mm). Performance of the MLC was checked by inspection of the DynaLog files created by the linear accelerator during the delivery of each dynamic field. The absolute difference between the calculated and measured isocenter doses for all the IMRS plans was 2.5% ± 2.1%. The gamma evaluation method resulted in high average passing rates of 98.9% ± 1.4% (red channel) and 98.9% ± 1.5% (blue and green channels). DynaLog file analysis revealed a maximum root mean square error of 0.46 mm. According to our results, we conclude that the Eclipse/AAA algorithm provides accurate cranial IMRS dose distributions that may be accurately delivered by a Varian linac equipped with a Millennium 120 MLC.

  19. A dosimetric evaluation of the Eclipse AAA algorithm and Millennium 120 MLC for cranial intensity-modulated radiosurgery

    International Nuclear Information System (INIS)

    The aim of this study is to assess the accuracy of a convolution-based algorithm (anisotropic analytical algorithm [AAA]) implemented in the Eclipse planning system for intensity-modulated radiosurgery (IMRS) planning of small cranial targets by using a 5-mm leaf-width multileaf collimator (MLC). Overall, 24 patient-based IMRS plans for cranial lesions of variable size (0.3 to 15.1 cc) were planned (Eclipse, AAA, version 10.0.28) using fixed field-based IMRS produced by a Varian linear accelerator equipped with a 120 MLC (5-mm width on central leaves). Plan accuracy was evaluated according to phantom-based measurements performed with radiochromic film (EBT2, ISP, Wayne, NJ). Film 2D dose distributions were performed with the FilmQA Pro software (version 2011, Ashland, OH) by using the triple-channel dosimetry method. Comparison between computed and measured 2D dose distributions was performed using the gamma method (3%/1 mm). Performance of the MLC was checked by inspection of the DynaLog files created by the linear accelerator during the delivery of each dynamic field. The absolute difference between the calculated and measured isocenter doses for all the IMRS plans was 2.5% ± 2.1%. The gamma evaluation method resulted in high average passing rates of 98.9% ± 1.4% (red channel) and 98.9% ± 1.5% (blue and green channels). DynaLog file analysis revealed a maximum root mean square error of 0.46 mm. According to our results, we conclude that the Eclipse/AAA algorithm provides accurate cranial IMRS dose distributions that may be accurately delivered by a Varian linac equipped with a Millennium 120 MLC

  20. Nanomechanical and Thermophoretic Analyses of the Nucleotide-Dependent Interactions between the AAA(+) Subunits of Magnesium Chelatase.

    Science.gov (United States)

    Adams, Nathan B P; Vasilev, Cvetelin; Brindley, Amanda A; Hunter, C Neil

    2016-05-25

    In chlorophyll biosynthesis, the magnesium chelatase enzyme complex catalyzes the insertion of a Mg(2+) ion into protoporphyrin IX. Prior to this event, two of the three subunits, the AAA(+) proteins ChlI and ChlD, form a ChlID-MgATP complex. We used microscale thermophoresis to directly determine dissociation constants for the I-D subunits from Synechocystis, and to show that the formation of a ChlID-MgADP complex, mediated by the arginine finger and the sensor II domain on ChlD, is necessary for the assembly of the catalytically active ChlHID-MgATP complex. The N-terminal AAA(+) domain of ChlD is essential for complex formation, but some stability is preserved in the absence of the C-terminal integrin domain of ChlD, particularly if the intervening polyproline linker region is retained. Single molecule force spectroscopy (SMFS) was used to determine the factors that stabilize formation of the ChlID-MgADP complex at the single molecule level; ChlD was attached to an atomic force microscope (AFM) probe in two different orientations, and the ChlI subunits were tethered to a silica surface; the probability of subunits interacting more than doubled in the presence of MgADP, and we show that the N-terminal AAA(+) domain of ChlD mediates this process, in agreement with the microscale thermophoresis data. Analysis of the unbinding data revealed a most probable interaction force of around 109 pN for formation of single ChlID-MgADP complexes. These experiments provide a quantitative basis for understanding the assembly and function of the Mg chelatase complex. PMID:27133226

  1. The AAA+ proteins Pontin and Reptin enter adult age: From understanding their basic biology to the identification of selective inhibitors

    Directory of Open Access Journals (Sweden)

    Pedro M Matias

    2015-05-01

    Full Text Available Pontin and Reptin are related partner proteins belonging to the AAA+ (ATPases Associated with various cellular Activities family. They are implicated in multiple and seemingly unrelated processes encompassing the regulation of gene transcription, the remodeling of chromatin, DNA damage sensing and repair, and the assembly of protein and ribonucleoprotein complexes, among others. The 2nd International Workshop on Pontin and Reptin took place at the Instituto de Tecnologia Química e Biológica António Xavier in Oeiras, Portugal on October 10-12, 2014, and reported significant new advances on the mechanisms of action of these two AAA+ ATPases. The major points under discussion were related to the mechanisms through which these proteins regulate gene transcription, their roles as co-chaperones, and their involvement in pathophysiology, especially in cancer and ciliary biology and disease. Finally, they may become anticancer drug targets since small chemical inhibitors were shown to produce anti-tumor effects in animal models.

  2. The AAA ATPase Vps4 Plays Important Roles in Candida albicans Hyphal Formation and is Inhibited by DBeQ.

    Science.gov (United States)

    Zhang, Yahui; Li, Wanjie; Chu, Mi; Chen, Hengye; Yu, Haoyuan; Fang, Chaoguang; Sun, Ningze; Wang, Qiming; Luo, Tian; Luo, Kaiju; She, Xueping; Zhang, Mengqian; Yang, Dong

    2016-06-01

    Candida albicans is an opportunistic human pathogen, and its pathogenicity is associated with hyphal formation. Previous studies have shown that at neutral-to-alkaline pH, hyphal growth is dependent on the Rim101 pathway whose activation requires Snf7, a member of the ESCRT system. In this work, we described the purification and characterization of the C. albicans Vps4, an AAA ATPase required for recycling of the ESCRTs. Its role on hyphal growth has been investigated. Our data suggest deletion of Vps4 decreases overall hyphal growth at pH 7 and increases the growth of multiple hyphae induced by serum, which indicates that the ESCRTs may make a Rim101-independent contribution to hyphal growth. Furthermore, DBeQ, an inhibitor of the AAA ATPase p97, was shown to inhibit the ATPase activity of Vps4 with an IC50 of about 11.5 μM. To a less degree, it also inhibits hyphal growth. Our work may provide a new strategy to control C. albicans infection. PMID:26700222

  3. Unfolding the mechanism of the AAA+ unfoldase VAT by a combined cryo-EM, solution NMR study.

    Science.gov (United States)

    Huang, Rui; Ripstein, Zev A; Augustyniak, Rafal; Lazniewski, Michal; Ginalski, Krzysztof; Kay, Lewis E; Rubinstein, John L

    2016-07-19

    The AAA+ (ATPases associated with a variety of cellular activities) enzymes play critical roles in a variety of homeostatic processes in all kingdoms of life. Valosin-containing protein-like ATPase of Thermoplasma acidophilum (VAT), the archaeal homolog of the ubiquitous AAA+ protein Cdc48/p97, functions in concert with the 20S proteasome by unfolding substrates and passing them on for degradation. Here, we present electron cryomicroscopy (cryo-EM) maps showing that VAT undergoes large conformational rearrangements during its ATP hydrolysis cycle that differ dramatically from the conformational states observed for Cdc48/p97. We validate key features of the model with biochemical and solution methyl-transverse relaxation optimized spectroscopY (TROSY) NMR experiments and suggest a mechanism for coupling the energy of nucleotide hydrolysis to substrate unfolding. These findings illustrate the unique complementarity between cryo-EM and solution NMR for studies of molecular machines, showing that the structural properties of VAT, as well as the population distributions of conformers, are similar in the frozen specimens used for cryo-EM and in the solution phase where NMR spectra are recorded. PMID:27402735

  4. Triple-A syndrome with prominent ophthalmic features and a novel mutation in the AAAS gene: a case report

    Directory of Open Access Journals (Sweden)

    Stuart Caroline

    2004-06-01

    Full Text Available Abstract Background Triple-A syndrome (Allgrove syndrome is an autosomal recessive disorder characterized by adrenal insufficiency, alacrima, achalasia, and – occasionally – autonomic instability. Mutations have been found in the AAAS gene on 12q13. Case presentation We present the case of a 12 year-old boy with classic systemic features of triple-A syndrome and several prominent ophthalmic features, including: accommodative spasm, dry eye, superficial punctate keratopathy, and pupillary hypersensitivity to dilute pilocarpine. MRI showed small lacrimal glands bilaterally. DNA sequencing of PCR-amplified fragments from the 16 exons of the AAAS gene revealed compound heterozygosity for a new, out-of-frame 5-bp deletion in exon 15, c1368-1372delGCTCA, and a previously-described nonsense mutation in exon 9, c938C>T, R286X. Conclusions In addition to known ophthalmic manifestations, triple-A syndrome can present with accommodative dysregulation and ocular signs of autonomic dysfunction.

  5. Selection and characterization of gamma ray induced Bunchy Top Virus resistant mutants in banana cv. Lakatan (Musa sp. AA)

    International Nuclear Information System (INIS)

    Gamma irradiation coupled with in vitro technology was used to develop Banana Bunchy Top Virus (BBTV) resistance in banana cv. Lakatan (Musa sp. AA). A total of 6,012 plants regenerated from irradiated shoot cultures were subjected to BBTV artificial inoculation using the aphid vector (Pentalonia nigronervosa Coq.) in green house. From these, 64 mutant plants with varying degrees of resistance/tolerance reaction to BBTV were selected after 30 months of evaluation. The resistant mutant plants were induced from 5 to 30 Gy gamma rays. Twenty six plants showed no BBTV symptoms in both irradiated and first generation sucker plants (rated resistant). The other 38 plants showed limited BBTV symptoms (rated intermediate resistant). Yield and agronomic traits of some resistant plants were comparable to non-irradiated tissue culture plants. Shorter plant stature was also generated in some BBTV resistant mutant plants. First generation suckers from resistant plants were evaluated for second cycle stability of the resistance trait. (author)

  6. Oxygen isotope use in the metallogenetic study of the Pedra Preta W mine, Musa Granite, Para State, Brazil

    International Nuclear Information System (INIS)

    The Pedra Preta wolframite deposit constitutes the main reserve of the Amazonian region, Brazil. Recent metallogenetic studies realized in the mine, allowed to identify four hydrothermal events related to the precocious, late and final vein formation. The first are of the metamorphic origin and basically composed of quartz, topaz and sulfide; the late veins are related to the Musa granite intrusion and are composed of quartz, topaz, fluorite, micas, sulfides and oxides (principally wolframite); the final veins are essentially constituted by quartz. The paper determines the characteristics isotopic signatures of different minerals found in the Pedra Preta deposit. Fourteen samples were analysed, and the fluid isotopic composition were determined through the δ18 O measurement in quartz, micas and wolframite

  7. Antihypercholesterolemic and antioxidant effect of sterol rich methanol extract of stem of Musa sapientum (banana) in cholesterol fed wistar rats.

    Science.gov (United States)

    Dikshit, Piyush; Tyagi, Mool Kumar; Shukla, Kirtikar; Gambhir, Jasvindar K; Shukla, Rimi

    2016-03-01

    Musa sapientum Linn. (English 'Banana' family Musaceae), is a plant with nutritive, as well as medicinal value. Antihypercholesterolemic and antioxidant effect of methanolic extract of stem of this plant was investigated in hypercholesterolemic rats. Rats were made hypercholesterolemic by feeding cholesterol (100 mg/kg/day) suspended in soya oil. Treatment groups received extract at a dose of 10, 20 and 40 mg/kg/day in addition to cholesterol orally once daily. Fasting blood samples were collected before and after 6 weeks treatment. Animals were sacrificed and liver stored at -80 °C. Total cholesterol, HDL-cholesterol and triacylglycerol were estimated in blood. Malondialdehyde, reduced glutathione, superoxide dismutase and catalase were measured in blood and liver. Total lipids, HMG CoA redutase and lipoprotein lipase were investigated in liver. Most effective dose was found to be 20 mg/kg/day. Rise in total cholesterol, LDL + VLDL-cholesterol and triacylglycerol in animals receiving only cholesterol was 179 %, 417 % and 74 % respectively, while in animals receiving 20 mg/kg dose rise in these parameters was restricted to 40 %, 106 % and 24 %. HDL-cholesterol decreased by 12 % in extract treated group, while it decreased to 36 % in untreated hypercholesterolemic rats. Malonaldialdehyde, marker of lipid peroxidation decreased while reduced glutathione and enzymes superoxide dismutase and catalase increased significantly in blood and liver (p < 0.01). Total lipids in liver decreased and enzymes of lipid metabolism viz. HMG CoA redutase and lipoprotein lipase were restored to near normal. Gas chromatography mass spectroscopy indicated high content of sterols in extract. Study demonstrated that methanol extract of stem of Musa sapientum has significant antihypercholesterolemic and antioxidant effects. PMID:27570294

  8. "Dos polos tiene la tierra, / universal movimiento" : dineros, musas y porfías en la última bibliografía lopesca

    OpenAIRE

    García-Bermejo Giner, Miguel

    2015-01-01

    Obres ressenyades: Carmen SANZ AYÁN, Hacer escena: capítulos de historia de la empresa teatral en el Siglo de Oro. Madrid, Real Academia de la Historia, 2013 ; Alejandro GARCÍA REIDY, Las musas rameras. Oficio dramático y conciencia profesional en Lope de Vega. Madrid-Frankfurt, Iberoamericana-Vervuert, 2013; Antonio CORTIJO OCAÑA, La porfía: identidad personal y nacional en Lope de Vega. Barcelona, Anthropos, 2013.

  9. Antifungal Activities of Crude Extractum from Camellia semiserrata Chi (Nanshancha) Seed Cake Against Colletotrichum musae, Colletotrichum gloeosporioides and Penicillium italicum in vitro and in vivo Fruit Test

    OpenAIRE

    Meng, Xiangchun; Li, Jun; Bi, Fangcheng; Zhu, Lixue; Ma, Zhiyu

    2015-01-01

    Antifungal activities of crude extractum of Nanshancha Seed Cake (NSC), to inactivate postharvest pathogens were investigated. Highest inhibitory rate was found against C. musae, C. gloeosporioides and C. papaya P.Henn, which was much stronger than that by tea saponin. Compared to tea saponin, effects of NSC extractum was relatively weak and similar on C. gloeosporioides Penzig and P. italicum. In an in vivo study, best controlling effects by NSC extractum was found with banana anthracnose di...

  10. Petrology of the anorogenic, oxidised Jamon and Musa granites, Amazonian Craton: implications for the genesis of Proterozoic A-type granites

    Science.gov (United States)

    Dall'Agnol, Roberto; Rämö, O. Tapani; de Magalhães, Marilia Sacramento; Macambira, Moacir José Buenano

    1999-03-01

    The 1.88 Ga Jamon and Musa granites are magnetite-bearing anorogenic, A-type granites of Paleoproterozoic age. They intrude the Archaean rocks of the Rio Maria Granite-Greenstone Terrain in the eastern part of the Amazonian Craton in northern Brazil. A suite of biotite±amphibole monzogranite to syenogranite, with associated dacite porphyry (DP) and granite porphyry (GP) dykes, dominates in these subalkaline granites that vary from metaluminous to peraluminous and show high FeO/(FeO+MgO) and K 2O/Na 2O. In spite of their broad geochemical similarities, the Jamon and Musa granites show some significant differences in their REE patterns and in the behaviour of Y. The Jamon granites are related by fractional crystallisation of plagioclase, potassium feldspar, quartz, biotite, magnetite±amphibole±apatite±ilmenite. Geochemical modelling and Nd isotopic data indicate that the Archaean granodiorites, trondhjemites and tonalites of the Rio Maria region are not the source of the Jamon Granite and associated dyke magmas. Archaean quartz diorites, differentiated from the mantle at least 1000 m.y. before the emplacement of the granites, have a composition adequate to generate DP and the hornblende-biotite monzogranite magmas by different degrees of partial melting. A larger extent of amphibole fractionation during the evolution of the Musa pluton can explain some of the observed differences between it and the Jamon pluton. The studied granites crystallised at relatively high fO 2 and are anorogenic magnetite-series granites. In this aspect, as well as concerning geochemical characteristics, they display many affinities with the Proterozoic A-type granites of south-western United States. The Jamon and Musa granites differ from the anorthosite-mangerite-charnockite-rapakivi granite suites of north-eastern Canada and from the reduced rapakivi granites of the Fennoscandian Shield in several aspects, probably because of different magmatic sources.

  11. The Use of Alternate Ligno-cellulosic Raw Materials Banana (Musa sapientum) Ankara (Calotropis procera ) and Pineapple (Ananas comosus ) in Handmade Paper & their Blending with Waste Paper.

    OpenAIRE

    Atul Kumar

    2013-01-01

    The studies were made to established suitability of lingo-cellulosic raw materials namely leaf fibreBanana (Musa Sapientum), bast fibre Ankara (Calotropis Procera), & leaf fibre Pineapple (Ananas Comosus) for making pulps for handmade paper industry. This should help in providing a cost effective, good quality cellulosic raw material as an alternate to cost prohibitive traditionally used cotton hosiery waste traditionally used for manufacturing good quality handmade paper & it’s products. Th...

  12. Control of Anthracnose Caused by Colletotrichum musae on Curcuma alismatifolia Gagnep. Using Antagonistic Bacillus spp.

    OpenAIRE

    Supuk Mahadtanapuk; Mondhon Sanguansermsri; Robert W. Cutler; Vicha Sardsud; Somboon Anuntalabhochai

    2007-01-01

    Over 400 bacterial strains, isolated from leaf surfaces of Curcuma alismatifolia Gagnep. and hot springs in the Chiang Mai province of northern Thailand, were screened in vitro for antagonistic activity against Colletotrichum musae, an anthracnose fungus. Three isolates provided greater than 75% growth inhibition of the fungus in vitro and were identified as Bacillus licheniformis, B. amyloliquefaciens and B. subtilis. Using in planta tests, B. amyloliquefaciens and B. subtilis were shown to ...

  13. OA02.02. Phytochemical constituents and antioxidative property of Musa acuminata flower as green corrosion inhibitor of mild steel in acid media

    OpenAIRE

    N. Gunavathy; ,

    2013-01-01

    Purpose: Corrosion is a major problem that must be confronted for safety, environmental and economic reasons. Organic compounds used as corrosion inhibitors are expensive, toxic and non biodegradable. The present investigation deals with the aim of finding eco friendly and cost effective natural inhibitor. The adsorption characteristic of phytochemical of Musa acuminata ‘nendran’ flower [MAN (F)] was studied to assess the mechanism of corrosion inhibition. Method: The inhibitive effect MAN (F...

  14. Chemical Composition, Nutritive and Energetic Value of Plantain (Musa ssp.) Hybrids CRBP 14, CRBP 39, FHIA 17, FHIA 21and Orishele Variety

    OpenAIRE

    Coulibaly, S.; Nemlin, GJ.; Kamenan, A.

    2007-01-01

    Chemical characteristics as well as contents in main components such as dry matter, sugars, lipids proteins and some minerals were determined in four banana hybrids CRBP 14, CRBP 39, FHIA 17, FHIA 21 and Orishele variety (used as control). Titrable acidity, pH, and calorific energy of these various cultivars of banana (Musa ssp.) were also determined. The results indicated that Orishele variety had the highest content of dry matter, total carbohydrate and potassium. On the other hand, CRBP 39...

  15. Subtitusi Tepung Pisang Awak (Musa Paradisiaca Var Awak) dan Ikan Lele Dumbo (Clarias Garipinus) Dalam Pembuatan Biskuit Serta Uji Daya Terimanya

    OpenAIRE

    Sari, Rini Puspa

    2015-01-01

    Banana ‘awak’ ripe flour (Musa paradisiaca var. Awak) and dumbo catfish (Clarias Garapinus) can be processed into biscuit. Biscuit is one of additional food that can full fill the needs of toddler nutrient. Biscuits had sweet taste and interesting shape. This research purpose to determine the acceptability test and nutritional content of biscuits substitution banana ‘awak’ flour and dumbo catfish. The type of research was an experiment conducted by completely randomized design using two fa...

  16. Sinergi Antara Nematoda Radopholus similis Dengan Jamur Fusarium oxysporum f.sp. cubense Terhadap Laju Serangan Layu Fusarium Pada Beberapa Kultivar Pisang (Musa sp ) Di Lapangan

    OpenAIRE

    Hasibuan, M. Alam Fauzi

    2010-01-01

    M Alam Fauzi Hasibuan SYNERGY BETWEEN Rhadopholus similis AND Fusarium oxysporum f.sp. cubense CONCERNING Fusarium wilt on banana (Musa sp) IN THE FIELD. Under suvervition of Mr. Ir. Lahmuddin Lubis, MP, as chif, and Mr. Ir. Mukhtar Iskandar Pinem, M. Agr, as member. This research was conducted at the Laboratory of Plant Diseases and Experimental Farm Faculty of Agriculture, University of North Sumatra, Medan. Starting from december 2009 until May 2010. This research aims to know Fusari...

  17. Acclimatation de vitroplants de bananier Musa sp. en culture hydroponique: impact de différentes concentrations en cuivre sur la croissance des vitroplants

    OpenAIRE

    Mazinga, MK.; Van Koninckxloo, M.; Godoy Jara, M.; Baboy Longanza, L.; Louvieaux, J.

    2013-01-01

    Acclimatation of Banana's Vitroplants (Musa sp.) in Hydroponic Culture: Effects of Different Concentrations Copper on Growth of Vitroplants. Constraints due to copper and consequences of its accumulation in acclimatized banana in vitroplants have been studied in hydroponic culture. 0-100- 500-1000 ppm copper was added to the nutrient medium in hydroponic culture. At 1000 ppm, copper was accumulated in the roots but not in the aerial parts. Surprisingly, biomass of shoots and roots was augment...

  18. Pareto front analysis of 6 and 15 MV dynamic IMRT for lung cancer using pencil beam, AAA and Monte Carlo

    DEFF Research Database (Denmark)

    Ottosson, R O; Hauer, Anna Karlsson; Behrens, C.F.

    2010-01-01

    are conventionally used for lung treatments. The aim of this study was to analyze the advantages and disadvantages of dynamic IMRT treatment planning for high and low photon energy in order to assess if deviating from the conventional low energy approach could be favorable in some cases. Furthermore, the influence...... of motion on the dose distribution was investigated. Four non-small cell lung cancer cases were selected for this study. Inverse planning was conducted using Varian Eclipse. A total number of 31 dynamic IMRT plans, distributed amongst the four cases, were created ranging from PTV conformity weighted...... to normal tissue sparing weighted. All optimized treatment plans were calculated using three different calculation algorithms (PBC, AAA and MC). In order to study the influence of motion, two virtual lung phantoms were created. The idea was to mimic two different situations: one where the GTV is located...

  19. Pareto front analysis of 6 and 15 MV dynamic IMRT for lung cancer using pencil beam, AAA and Monte Carlo

    DEFF Research Database (Denmark)

    Ottosson, R O; Hauer, Anna Karlsson; Behrens, C.F.

    2010-01-01

    conventionally used for lung treatments. The aim of this study was to analyze the advantages and disadvantages of dynamic IMRT treatment planning for high and low photon energy in order to assess if deviating from the conventional low energy approach could be favorable in some cases. Furthermore, the influence...... of motion on the dose distribution was investigated. Four non-small cell lung cancer cases were selected for this study. Inverse planning was conducted using Varian Eclipse. A total number of 31 dynamic IMRT plans, distributed amongst the four cases, were created ranging from PTV conformity weighted...... to normal tissue sparing weighted. All optimized treatment plans were calculated using three different calculation algorithms (PBC, AAA and MC). In order to study the influence of motion, two virtual lung phantoms were created. The idea was to mimic two different situations: one where the GTV is...

  20. Comparison of Acuros (AXB) and Anisotropic Analytical Algorithm (AAA) for dose calculation in treatment of oesophageal cancer: effects on modelling tumour control probability

    International Nuclear Information System (INIS)

    To investigate systematic changes in dose arising when treatment plans optimised using the Anisotropic Analytical Algorithm (AAA) are recalculated using Acuros XB (AXB) in patients treated with definitive chemoradiotherapy (dCRT) for locally advanced oesophageal cancers. We have compared treatment plans created using AAA with those recalculated using AXB. Although the Anisotropic Analytical Algorithm (AAA) is currently more widely used in clinical routine, Acuros XB (AXB) has been shown to more accurately calculate the dose distribution, particularly in heterogeneous regions. Studies to predict clinical outcome should be based on modelling the dose delivered to the patient as accurately as possible. CT datasets from ten patients were selected for this retrospective study. VMAT (Volumetric modulated arc therapy) plans with 2 arcs, collimator rotation ± 5-10° and dose prescription 50 Gy / 25 fractions were created using Varian Eclipse (v10.0). The initial dose calculation was performed with AAA, and AXB plans were created by re-calculating the dose distribution using the same number of monitor units (MU) and multileaf collimator (MLC) files as the original plan. The difference in calculated dose to organs at risk (OAR) was compared using dose-volume histogram (DVH) statistics and p values were calculated using the Wilcoxon signed rank test. The potential clinical effect of dosimetric differences in the gross tumour volume (GTV) was evaluated using three different TCP models from the literature. PTV Median dose was apparently 0.9 Gy lower (range: 0.5 Gy - 1.3 Gy; p < 0.05) for VMAT AAA plans re-calculated with AXB and GTV mean dose was reduced by on average 1.0 Gy (0.3 Gy −1.5 Gy; p < 0.05). An apparent difference in TCP of between 1.2% and 3.1% was found depending on the choice of TCP model. OAR mean dose was lower in the AXB recalculated plan than the AAA plan (on average, dose reduction: lung 1.7%, heart 2.4%). Similar trends were seen for CRT plans

  1. Efectos de dos sistemas de producción en el contenido de minerales en el fruto de Musa AAB Simmonds Effects of two production systems in the mineral content in the fruit of Musa AAB Simmonds

    Directory of Open Access Journals (Sweden)

    José Luis Barrera-Violeth

    2011-12-01

    Full Text Available Los sistemas de producción orgánico y/o ecológico tienen como objetivo garantizar la sostenibilidad y la renovación de base natural, mediante la limitación del uso de productos de síntesis química para favorecer el ambiente y la salud humana. Teniendo en cuenta estas características, se estudió el efecto de los sistemas de producción orgánico y convencional sobre el contenido de nutrientes minerales del fruto del plátano Hartón (Musa AAB Simmonds, durante el proceso de maduración. Las determinaciones se realizaron en la Universidad de Córdoba (Montería, Colombia con el fin de establecer las diferencias en el contenido de minerales en los frutos en ambos sistemas y en diferentes estados de maduración. Se utilizó un diseño completamente al azar con arreglo factorial 2 x 5 y cinco repeticiones. Los factores fueron: sistema de producción y estado de maduración; los cinco niveles del segundo factor correspondieron a los grados de maduración: verde-oscuro (V, verde-claro (VC, amarillo-verde (AV, amarillo (A y muy amarillo (MA. Los resultados obtenidos muestran que entre ambos sistemas de producción y en distintos grados de maduración, se presentan diferencias significativas (P Organic production systems and/or ecological aim to ensure the sustainability and renewal of natural base by limiting chemical synthesis products favoring the environment and human health due to these benefits, we studied the effect of systems organic and conventional production on mineral nutrient content of the fruit of plantain (Musa AAB Simmonds during the ripening process. Measurements were performed at the University of Córdoba (Montería, Colombia in order to differentiate between the two systems, in varying states of maturity. A complete randomized design with 2 x 5 factorial arrangements with five replications was used. The factors were: production system and state of maturity, the five levels of the second factor corresponded to the following

  2. Micromicetos del suelo de una plantación de plátano (Musa paradisiaca en Teapa, Tabasco, México Soil microfungi from a banana (Musa paradisiaca plantation in Teapa, Tabasco, Mexico

    Directory of Open Access Journals (Sweden)

    Mariana Del Olmo-Ruiz

    2010-04-01

    Full Text Available Se analizó la micobiota presente en el suelo de un cultivo de plátano (Musa paradisiaca L. en el municipio de Teapa en el estado de Tabasco, México. Los objetivos fueron conocer las especies habitantes de este suelo y la dinámica de la comunidad con respecto a la composición de especies a lo largo de un año. Se llevaron a cabo 4 muestreos durante temporadas distintas y los aislamientos se realizaron mediante la técnica de dilución en placa. Se recuperaron 91 colonias, correspondientes a 30 especies diferentes de hongos mitospóricos, de las cuales únicamente Aspergillus flavus Link, Fusarium nivale (Fr. Ces y Trichoderma harzianum Rifai fueron especies residentes de ese suelo y el resto, esporádicas. El índice de similitud de Sorensen evidenció una sucesión de especies entre las temporadas de muestreo. Del total de especies determinadas en este estudio, 8 no habían sido encontradas como habitantes de suelos mexicanos. Asimismo, se mostró que el suelo analizado es un hábitat muy rico en micromicetos y que es necesario incrementar los esfuerzos de colecta para tener un mayor conocimiento sobre la diversidad en nuestro país.In this study, we analyzed the soil microfungal community from a banana (Musa paradisiaca L. plantation in Teapa, a municipality of Tabasco State in Mexico. The objectives were to determine the fungal species present and to analyze the community composition throughout an entire year. We performed 4 samplings during different seasons and the fungal isolates were recovered using the dilution plate technique. We isolated 91 strains from 30 different mitosporic species where only Aspergillus flavus Link, Fusarium nivale (Fr. Ces and Trichoderma harzianum Rifai were considered as resident species while the remaining species were sporadic. The Sorensen similarity index suggested that species succession had occurred among the sampling seasons. From all the species identified in this survey, 8 had not been found as

  3. Antioxidant properties of aqueous extracts of unripe Musa paradisiaca on sodium nitroprusside induced lipid peroxidation in rat pancreas in vitro

    Institute of Scientific and Technical Information of China (English)

    Sidiqat Adamson Shodehinde; Ganiyu Oboh

    2013-01-01

    To evaluate and compare antioxidant activities of the aqueous extracts of unripe plantain (Musa paradisiaca), assess their inhibitory action on sodium nitroprusside induced lipid peroxidation in rat pancreas in vitro and to characterize the main phenolic constituents of the plantain products using gas chromatography analysis. Methods: Aqueous extracts of plantain products (raw, elastic pastry, roasted and boiled) flour of 0.1 g/mL (each) were used to determine their total phenol, total flavonoid, 1,1 diphenyl-2 picrylhydrazyl (DPPH) and hydroxyl (OH) radical scavenging ability. The inhibitory effect of the extracts on sodium nitroprusside induced lipid peroxidation was also determined. Results: The results revealed that all the aqueous extracts showed antioxidant activity. The boiled flour had highest DPPH and OH radical scavenging ability while raw flour had the highest Fe2+ chelating ability, sodium nitroprusside inhibitory effect and vitamin C content. The antioxidant results showed that elastic pastry had the highest total phenol and total flavonoid content. Characterization of the unripe plantain products for polyphenol contents using gas chromatography showed varied quantity of apigenin, myricetin, luteolin, capsaicin, isorhaemnetin, caffeic acid, kampferol, quercetin, p-hydroxybenzoic acid, shogaol, glycitein and gingerol per product on the spectra. Conclusions: Considering the antioxidant activities and ability to inhibit lipid peroxidation of unripe plantain, this could justify their traditional use in the management/prevention of diseases related to stress.

  4. Selection parameters for resistance to Fusarium oxysporum f. sp. cubense race 1 and race 4 on diploid banana (Musa acuminata)

    International Nuclear Information System (INIS)

    Shoot tip cultures from banana clones susceptible and resistant to Fusarium oxysporum f. sp. cubense (FOC) race 1 and race 4 were grown in vitro in the presence of different concentrations of fusaric acid and fungal crude filtrates or inoculated with a conidial suspension of FOC to assess correlation between in vivo and in vitro behaviour. Explants were susceptible to both filtrate and fusaric acid irrespective of their known field resistance/susceptibility response. No clear linkage between in vivo and in vitro behaviour was observed, and our results suggest that the use of crude filtrate or non-host- specific toxin (fusaric acid) in a screening programme for selecting a novel resistant genotype of Musa to FOC is not feasible. When peroxidase activity was used as a parameter to discriminate between susceptibility and tolerance, the results were in good agreement with the field response of host plant to pathogens. Early enzymatic activity increased in the incompatible host-pathogen interaction but not in the compatible interaction. (author)

  5. Effet des différentes balances hormonales sur la microprogation du Cv. Hybride FHIA-01 (Musa sp.

    Directory of Open Access Journals (Sweden)

    Mazinga, KM.

    2013-01-01

    Full Text Available Effects of Different Hormonal Balances on the Microprogation of Cv. Hybrid FHIA-01 (Musa sp.. Different hormonal balances were evaluated to develop a growth medium to effectively multiply the FHIA-01 hybrid in vitro The most striking results of this study are: the almost complete inhibition of the shoot proliferation by BAP; the total root inhibition by the concentration 10 μM BAP+10 μM MemTR and the improvement of the mean shoot proliferation (5,4 shoots/explants by adding the same concentration. Adding MemTR improves the hormonal balance for the proliferation of shoots of FHIA-01. At least 1 μM of IAA is needed as auxin source, to reach the same result. In this study, we identified some factors related to the experimental conditions, such as light and darkness that seemed to be necessary for the organogenesis of FHIA-01. The cultures had to be placed 15 days in darkness and 15 days in light conditions.

  6. Evaluation of physico-chemical and antioxidant properties in different varieties of banana (musa acuminata), indigenous to pakistan

    International Nuclear Information System (INIS)

    The antioxidant capacity, phenolic and flavonoid contents, and physico-chemical analysis on the pulp of three different varieties of Musa acuminata, were studied namely Cavendish basrai, Grand naine and Plantain, collected from Gharo, Adam Khas Kheli Road near Karachi (Pakistan). DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging, reducing power (RPA) and phosphomolybdenum assays were used for determination of their antioxidant capacity. Cavendish basrai characterized the highest antioxidant capacity among three varieties, DPPH (82.46 % inhibition at 0.1 mM conc.), RPA (43.59 mg/100 g) and phosphomolybdenum (38.90 mg/100 g) in methanolic extract and DPPH (67.27 % inhibition at 0.1 mM conc.), RPA (27.03 mg/100 g) and phosphomolybdenum (24.27 mg/100 g) in water extract. The phenolic (83.04 mg/100 g, 19.50 mg/100 g) and flavonoid contents (11.66 mg/100 g, 4.77 mg/100 g) were also high in Cavendish basrai in methanolic and water extracts, respectively showed the direct relation of antioxidant capacity to the phenolic and flavonoid contents, and the DPPH assay revealed more power full assay for determination of antioxidant capacity among these assays. In correlation with antioxidant capacity, Plantain showed comparatively high physico-chemical characteristics revealed high nutritional contents such as total dry matter, total sugar contents, TSS, titratable acidity and % NaCl. (author)

  7. Levaduras autóctonas con capacidad fermentativa en la producción de etanol a partir de pulpa de excedentes de plátano Musa (AAB Simmonds) en el departamento de Córdoba, Colombia

    OpenAIRE

    Luis Oviedo Zumaqué; Cecilia Lara Mantilla; Mauricio Mizger Pantoja

    2009-01-01

    Autoctonous yeasts having fermentation ability in producing ethanol from Musa (AAB Simmonds) plantain surplus pulp in the Córdoba department of Colombia Resumen: Se evaluó la capacidad fermentativa de levaduras nativas de la zona costanera del departamento de Córdoba, Colombia, para la obtención de etanol a partir de la pulpa de excedentes de plátano Musa (AAB Simmonds), con el objetivo de encontrar cepas eficientes. Los microorganismos utilizados correspondieron a las especies: Kloeckera sp,...

  8. Granules morphology and rheological behavior of green banana (Musa cavendishii) and corn (Zea mays) starch gels Morfologia dos grânulos e comportamento reológico dos géis de amido de banana verde (Musa cavendishii) e milho (Zea mays)

    OpenAIRE

    Dayane Rosalyn Izidoro; Bogdan Demczuk Junior; Charles Windson Isidoro Haminiuk; Maria Rita Sierakowski; Renato João Sossela de Freitas; Agnes de Paula Scheer

    2007-01-01

    In this work, it was used starch obtained from green banana (Musa cavendishii) and commercial corn (Zea mays) starch in order to compare the granule morphology and the rheological behavior of these gel-starches. Images of starch granules morphology were obtained from scanning electron microscope (SEM). The banana starch granules presented an oval and ellipsoidal shape with irregular diameters. Neverthless, the granules of corn starch showed a poliedric shape, with different sizes. The rheolog...

  9. Structural insights into the Escherichia coli lysine decarboxylases and molecular determinants of interaction with the AAA+ ATPase RavA

    Science.gov (United States)

    Kandiah, Eaazhisai; Carriel, Diego; Perard, Julien; Malet, Hélène; Bacia, Maria; Liu, Kaiyin; Chan, Sze W. S.; Houry, Walid A.; Ollagnier de Choudens, Sandrine; Elsen, Sylvie; Gutsche, Irina

    2016-01-01

    The inducible lysine decarboxylase LdcI is an important enterobacterial acid stress response enzyme whereas LdcC is its close paralogue thought to play mainly a metabolic role. A unique macromolecular cage formed by two decamers of the Escherichia coli LdcI and five hexamers of the AAA+ ATPase RavA was shown to counteract acid stress under starvation. Previously, we proposed a pseudoatomic model of the LdcI-RavA cage based on its cryo-electron microscopy map and crystal structures of an inactive LdcI decamer and a RavA monomer. We now present cryo-electron microscopy 3D reconstructions of the E. coli LdcI and LdcC, and an improved map of the LdcI bound to the LARA domain of RavA, at pH optimal for their enzymatic activity. Comparison with each other and with available structures uncovers differences between LdcI and LdcC explaining why only the acid stress response enzyme is capable of binding RavA. We identify interdomain movements associated with the pH-dependent enzyme activation and with the RavA binding. Multiple sequence alignment coupled to a phylogenetic analysis reveals that certain enterobacteria exert evolutionary pressure on the lysine decarboxylase towards the cage-like assembly with RavA, implying that this complex may have an important function under particular stress conditions. PMID:27080013

  10. In-situ monitoring of blood glucose level for dialysis machine by AAA-battery-size ATR Fourier spectroscopy

    Science.gov (United States)

    Hosono, Satsuki; Sato, Shun; Ishida, Akane; Suzuki, Yo; Inohara, Daichi; Nogo, Kosuke; Abeygunawardhana, Pradeep K.; Suzuki, Satoru; Nishiyama, Akira; Wada, Kenji; Ishimaru, Ichiro

    2015-07-01

    For blood glucose level measurement of dialysis machines, we proposed AAA-battery-size ATR (Attenuated total reflection) Fourier spectroscopy in middle infrared light region. The proposed one-shot Fourier spectroscopic imaging is a near-common path and spatial phase-shift interferometer with high time resolution. Because numerous number of spectral data that is 60 (= camera frame rare e.g. 60[Hz]) multiplied by pixel number could be obtained in 1[sec.], statistical-averaging improvement realize high-accurate spectral measurement. We evaluated the quantitative accuracy of our proposed method for measuring glucose concentration in near-infrared light region with liquid cells. We confirmed that absorbance at 1600[nm] had high correlations with glucose concentrations (correlation coefficient: 0.92). But to measure whole-blood, complex light phenomenon caused from red blood cells, that is scattering and multiple reflection or so, deteriorate spectral data. Thus, we also proposed the ultrasound-assisted spectroscopic imaging that traps particles at standing-wave node. Thus, if ATR prism is oscillated mechanically, anti-node area is generated around evanescent light field on prism surface. By elimination complex light phenomenon of red blood cells, glucose concentration in whole-blood will be quantify with high accuracy. In this report, we successfully trapped red blood cells in normal saline solution with ultrasonic standing wave (frequency: 2[MHz]).

  11. Quantum corrections to ward identities of chronological AVV- and AAA-current correlators for nondegenerate many-fermion systems in the four-dimensional world

    International Nuclear Information System (INIS)

    The explicit form of nontrivial quantum corrections to Ward identities for AVV- and AAA-current correlators in the four-dimensional world for nondegenerate many-fermion systems of general type is obtained. The characteristics of all nontrivial quantum corrections for nondegenerate two-flavour fermion systems are classified and described. In particular, the well-known results follow from ours for the trivial quantum corrections (anomalies) in the case of the degenerate spectrum of fermion masses

  12. Elements in nucleotide sensing and hydrolysis of the AAA+ disaggregation machine ClpB: a structure-based mechanistic dissection of a molecular motor

    International Nuclear Information System (INIS)

    High-resolution crystal structures together with mutational analysis and transient kinetics experiments were utilized to understand nucleotide sensing and the regulation of the ATPase cycle in an AAA+ molecular motor. ATPases of the AAA+ superfamily are large oligomeric molecular machines that remodel their substrates by converting the energy from ATP hydrolysis into mechanical force. This study focuses on the molecular chaperone ClpB, the bacterial homologue of Hsp104, which reactivates aggregated proteins under cellular stress conditions. Based on high-resolution crystal structures in different nucleotide states, mutational analysis and nucleotide-binding kinetics experiments, the ATPase cycle of the C-terminal nucleotide-binding domain (NBD2), one of the motor subunits of this AAA+ disaggregation machine, is dissected mechanistically. The results provide insights into nucleotide sensing, explaining how the conserved sensor 2 motif contributes to the discrimination between ADP and ATP binding. Furthermore, the role of a conserved active-site arginine (Arg621), which controls binding of the essential Mg2+ ion, is described. Finally, a hypothesis is presented as to how the ATPase activity is regulated by a conformational switch that involves the essential Walker A lysine. In the proposed model, an unusual side-chain conformation of this highly conserved residue stabilizes a catalytically inactive state, thereby avoiding unnecessary ATP hydrolysis

  13. Elements in nucleotide sensing and hydrolysis of the AAA+ disaggregation machine ClpB: a structure-based mechanistic dissection of a molecular motor

    Energy Technology Data Exchange (ETDEWEB)

    Zeymer, Cathleen, E-mail: cathleen.zeymer@mpimf-heidelberg.mpg.de; Barends, Thomas R. M.; Werbeck, Nicolas D.; Schlichting, Ilme; Reinstein, Jochen, E-mail: cathleen.zeymer@mpimf-heidelberg.mpg.de [Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg (Germany)

    2014-02-01

    High-resolution crystal structures together with mutational analysis and transient kinetics experiments were utilized to understand nucleotide sensing and the regulation of the ATPase cycle in an AAA+ molecular motor. ATPases of the AAA+ superfamily are large oligomeric molecular machines that remodel their substrates by converting the energy from ATP hydrolysis into mechanical force. This study focuses on the molecular chaperone ClpB, the bacterial homologue of Hsp104, which reactivates aggregated proteins under cellular stress conditions. Based on high-resolution crystal structures in different nucleotide states, mutational analysis and nucleotide-binding kinetics experiments, the ATPase cycle of the C-terminal nucleotide-binding domain (NBD2), one of the motor subunits of this AAA+ disaggregation machine, is dissected mechanistically. The results provide insights into nucleotide sensing, explaining how the conserved sensor 2 motif contributes to the discrimination between ADP and ATP binding. Furthermore, the role of a conserved active-site arginine (Arg621), which controls binding of the essential Mg{sup 2+} ion, is described. Finally, a hypothesis is presented as to how the ATPase activity is regulated by a conformational switch that involves the essential Walker A lysine. In the proposed model, an unusual side-chain conformation of this highly conserved residue stabilizes a catalytically inactive state, thereby avoiding unnecessary ATP hydrolysis.

  14. Competitive and Noncompetitive Batch Sorption Studies of Aqueous Cd(II and Pb(II Uptake onto Coffea canephora Husks, Cyperus papyrus Stems, and Musa spp. Peels

    Directory of Open Access Journals (Sweden)

    G. K. Bakyayita

    2015-01-01

    Full Text Available Coffea canephora, Cyperus papyrus, and Musa spp. were studied for competitive and noncompetitive removal of aqueous Cd2+ and Pb2+. The optimal conditions were pH 4.5 and agitation time 3.0 hours. Biomass constituent ions showed no interference effects whereas cation exchange capacity values corresponded to the sorption efficiencies. XRD spectroscopy revealed surface oxygen and nitrogen groups that provide binding sites for metal ions. The maximum sorption efficiency ranges for metal ions in noncompetitive media were 95.2–98.7% for C. canephora, 42.0–91.3% for C. papyrus, and 79.9–92.2% for Musa spp. and in competitive sorption 90.8–98.0% for C. canephora, 19.5–90.4% for C. papyrus, and 56.4–89.3% for Musa spp. The Pb2+ ions uptake was superior to that of Cd2+ ions in competitive and noncompetitive media. In competitive sorption synergistic effects were higher for Cd2+ than Pb2+ ions. The pseudo-second-order kinetic model fitted experimental data with 0.917≤R2≥1.000 for Pb2+ ions and 0.711≤R2≥0.999 for Cd2+ ions. The Langmuir model fitted noncompetitive sorption data with 0.769≤R2≥0.999; moreover the Freundlich model fitted competitive sorption data with 0.867≤R2≥0.989. Noncompetitive sorption was monolayer chemisorption whereas competitive sorption exhibited heterogeneous sorption mechanisms.

  15. The stinging Apidae and Vespidae (Hymenoptera:Apocrita) in Iranian islands, Qeshm, Abu-Musa, Great Tunb and Lesser Tunb on the Persian Gulf

    Institute of Scientific and Technical Information of China (English)

    Mehdi Khoobdel; Maryam Tavassoli; Mehdi Salari; Fateme Firozi

    2014-01-01

    Objective: To study the stinging flying Hymenoptera (Apidae and Vespidae) fauna in four Iranian Islands, Qeshm, Greater Tunb, Lesser Tunb and Abu-Musa on the Persian Gulf.Methods:hashing from March 2011 to July 2012. The flies were captured by used of Malaise trap, fly trap, bottle trap and insect net-Results: In this study, 11 species of stinging Hymenoptera were reported for the first time in Persian Gulf region.Conclusions:Some of this species such as Vespa orientalis and Polistes olivaceus are more common in the Persian Gulf islands and can cause clinical problem to islands resident and travelers.

  16. Histology of Callogenesis in Diploid Bananas (Musa acuminata, AA Group) �Kluai Sa� and �Kluai Leb Mu Nang�

    OpenAIRE

    Kamnoon KANCHANAPOOM; Korn KORAPATCHAIKUL

    2012-01-01

    Yellow compact calluses were induced from in vitro-grown shoot tips of diploid bananas (Musa acuminata, AA group) �Kluai Sa� and �Kluai Leb Mu Nang� on a modified Murashige and Skoog (MS) medium containing 100 mg/L malt extract, 50 mg/L proline, 50 mg/L cysteine, 100 mg/L glutamine, 1 mg/L biotin, 7 mg/L Dicamba and 2 mg/L TDZ. Green shoot buds were induced after transfer of the yellow compact calluses to the same MS medium but supplemented with 1 mg/L NAA and 3 mg/L BA and plant regeneration...

  17. Verification of Rapid Arc™ planning with AAA algorithm using an inhomogeneous 3D phantom; Verificacao de planejamentos de RapidArc™ com algoritmo AAA usando um fantoma heterogeneo 3D

    Energy Technology Data Exchange (ETDEWEB)

    Trindade, Cassia; Silva, Leonardo P.; Souza, Roberto S.; Batista, Delano V.L.; Martins, Lais P.; Santos, Maira R.; Garcia, Paulo L., E-mail: cassiatr@gmail.com [Instituto Nacional do Cancer (INCa), Rio de Janeiro, RJ (Brazil)

    2013-12-15

    New technologies have been developed to improve the quality assurance of the planning with modulated beams. One way to deal with the high costs of the dosimetry equipment was to develop a 3D phantom, using TLDs and radiochromic film, designed by the Radiotherapy Quality Program of INCa. The calculus was done using the AAA algorithm with heterogeneity correction, making the phantom rather heterogeneous. Five measurements related to the Rapid Arc™ planning were taken, once there was the phantom CT for optimization. The purpose of this work is a 3D verification of the dose distribution in the heterogeneous phantom. The mean deviation in planning target volumes was lower than ±5%. On the other side, the results dispersion for the others heterogeneities was higher, the maximum mean deviation obtained, for example, for the heterogeneity related to the bladder, was 7.41%. The maximum standard deviation found for both cases was around 9% for the target heterogeneity and 11% for the other heterogeneities. The phantom might be an interesting tool in order to verify the Rapid Arc™ planning, however, more statistical data is necessary as to achieve better results for the analysis of dose distribution. (author)heterogeneous phantom. The mean deviation in planning target volumes was lower than ±5%. On the other side, the results dispersion for the others heterogeneities was higher, the maximum mean deviation obtained, for example, for the heterogeneity related to the bladder, was 7.41%. The maximum standard deviation found for both cases was around 9% for the target heterogeneity and 11% for the other heterogeneities. The phantom might be an interesting tool in order to verify the Rapid Arc™ planning, however, more statistical data is necessary as to achieve better results for the analysis of dose distribution. (author)

  18. Evaluation of the Analytical Anisotropic Algorithm (AAA) in dose calculation for fields with non-uniform fluences considering heterogeneity correction; Avaliacao do Algoritmo Analitico Anisotropico (AAA) no calculo de dose para campos com fluencia nao uniforme considerando correcao de heterogeneidade

    Energy Technology Data Exchange (ETDEWEB)

    Bornatto, P.; Funchal, M.; Bruning, F.; Toledo, H.; Lyra, J.; Fernandes, T.; Toledo, F.; Marciao, C., E-mail: pricila_bornatto@yahoo.com.br [Hospital Erasto Gaertner (LPCC), Curitiba, PR (Brazil). Departamento de Radioterapia

    2014-08-15

    The purpose of this study is to evaluate the calculation of dose distribution AAA (Varian Medical Systems) for fields with non-uniform fluences considering heterogeneity correction. Five different phantoms were used with different density materials. These phantoms were scanned in the CT BrightSpeed (©GE Healthcare) upon the array of detectors MAPCHECK2 TM (Sun Nuclear Corporation) and irradiated in a linear accelerator 600 CD (Varian Medical Systems) 6MV and rate dose 400MU/min with isocentric setup. The fluences used were exported from IMRT plans, calculated by ECLIPSE™ planning system (Varian Medical Systems), and a 10x10 cm{sup 2} field to assess the heterogeneity correction for uniform fluence. The measured dose distribution was compared to the calculated by Gamma analysis with approval criteria of 3% / 3 mm and 10% threshold. The evaluation was performed using the software SNCPatient (Sun Nuclear Corporation) and considering absolute dose normalized at maximum. The phantoms best performers were those with low density materials, with an average of 99.2% approval. Already phantoms with plates of higher density material presented various fluences below 95% of the points approved. The average value reached 94.3%. It was observed a dependency between fluency and approved percentage points, whereas for the same fluency, 100% of the points have been approved in all phantoms. The approval criteria for IMRT plans recommended in most centers is 3% / 3mm with at least 95% of points approved, it can be concluded that, under these conditions, the IMRT plans with heterogeneity correction can be performed , however the quality control must be careful because the difficulty of the system to accurately predict the dose distribution in certain situations. (author)

  19. ARMAZENAMENTO E MATURAÇÃO DE BANANAS DO CULTIVAR PRATA (Musa sp EM SACOS DE POLIETILENO CONTENDO ABSORVENTE DE ETILENO STORAGE AND MATURATION OF BANANA (Musa sp. “PRATA” CULTIVAR IN POLYETHYLENE BAGS CONTAINING ETHYLENE ABSORBENT

    Directory of Open Access Journals (Sweden)

    Lincoln F. Zica

    2007-09-01

    Full Text Available

    Este trabalho teve como objetivo estudar um meio adequado de conservação de banana “Prata” (Musa sp., para as condições de mercado interno, utilizando polietileno como modificador do meio envolvente e como embalagem permanente do produtor ao consumidor. Para isto, utilizaram-se os seguintes tratamentos: Testemunha, sem embalagem; Plástico perfurado; Plástico perfurado, contendo absorvente; Plástico sem perfuração; Plástico sem perfuração, contendo absorvente. As bananas tratadas, foram acondicionadas em câmara com temperatura constante de 22 ± 1°C e umidade relativa de 90 ± 3%. Uma vez por dia era feita a leitura da cor da casca e a ventilação de câmara. Com auxílio das análises estatísticas dos dados obtidos concluiu-se que: a. Uso de absorvente de etileno dentro da embalagem plástica, sem perfuração, atrasa o início do amadurecimento, mas não impede que os frutos se tornem imprestáveis para o consumo. b. A melhor embalagem encontrada para ser usada do produtor ao consumidor foi a de plástico perfurado sem absorvente de etileno, que dá um ganho de aproximadamente cinco dias de conservação à temperatura de 22° C.

    This experiment had as its objective the study of an adequate way to conserve the banana “Prata” (Musa sp. in order to meet conditions of the national market. Poliethylene packaging from the producer to the consumer. For this, the following treatments were used: no packaging, perforated plastic, imperforated plastic containing an absorvent, imperforated plastic, imperforated plastic containing an absorvent. The treated banana were stored in a container with a constant temperature of 22 ± 1°C and a relative humidity of 90 ± 3%. Once a day a reading was taken of the skin color and the room was ventilated. With the help of statistical analysis of the obtained

  20. Experimental verification of the Acuros XB and AAA dose calculation adjacent to heterogeneous media for IMRT and RapidArc of nasopharygeal carcinoma

    International Nuclear Information System (INIS)

    Purpose: To compare the doses calculated by the Acuros XB (AXB) algorithm and analytical anisotropic algorithm (AAA) with experimentally measured data adjacent to and within heterogeneous medium using intensity modulated radiation therapy (IMRT) and RapidArc® (RA) volumetric arc therapy plans for nasopharygeal carcinoma (NPC). Methods: Two-dimensional dose distribution immediately adjacent to both air and bone inserts of a rectangular tissue equivalent phantom irradiated using IMRT and RA plans for NPC cases were measured with GafChromic® EBT3 films. Doses near and within the nasopharygeal (NP) region of an anthropomorphic phantom containing heterogeneous medium were also measured with thermoluminescent dosimeters (TLD) and EBT3 films. The measured data were then compared with the data calculated by AAA and AXB. For AXB, dose calculations were performed using both dose-to-medium (AXBDm) and dose-to-water (AXBDw) options. Furthermore, target dose differences between AAA and AXB were analyzed for the corresponding real patients. The comparison of real patient plans was performed by stratifying the targets into components of different densities, including tissue, bone, and air. Results: For the verification of planar dose distribution adjacent to air and bone using the rectangular phantom, the percentages of pixels that passed the gamma analysis with the ± 3%/3mm criteria were 98.7%, 99.5%, and 97.7% on the axial plane for AAA, AXBDm, and AXBDw, respectively, averaged over all IMRT and RA plans, while they were 97.6%, 98.2%, and 97.7%, respectively, on the coronal plane. For the verification of planar dose distribution within the NP region of the anthropomorphic phantom, the percentages of pixels that passed the gamma analysis with the ± 3%/3mm criteria were 95.1%, 91.3%, and 99.0% for AAA, AXBDm, and AXBDw, respectively, averaged over all IMRT and RA plans. Within the NP region where air and bone were present, the film measurements represented the dose close to

  1. Evaluation of the Analytical Anisotropic Algorithm (AAA) in dose calculation for fields with non-uniform fluences considering heterogeneity correction

    International Nuclear Information System (INIS)

    The purpose of this study is to evaluate the calculation of dose distribution AAA (Varian Medical Systems) for fields with non-uniform fluences considering heterogeneity correction. Five different phantoms were used with different density materials. These phantoms were scanned in the CT BrightSpeed (©GE Healthcare) upon the array of detectors MAPCHECK2 TM (Sun Nuclear Corporation) and irradiated in a linear accelerator 600 CD (Varian Medical Systems) 6MV and rate dose 400MU/min with isocentric setup. The fluences used were exported from IMRT plans, calculated by ECLIPSE™ planning system (Varian Medical Systems), and a 10x10 cm2 field to assess the heterogeneity correction for uniform fluence. The measured dose distribution was compared to the calculated by Gamma analysis with approval criteria of 3% / 3 mm and 10% threshold. The evaluation was performed using the software SNCPatient (Sun Nuclear Corporation) and considering absolute dose normalized at maximum. The phantoms best performers were those with low density materials, with an average of 99.2% approval. Already phantoms with plates of higher density material presented various fluences below 95% of the points approved. The average value reached 94.3%. It was observed a dependency between fluency and approved percentage points, whereas for the same fluency, 100% of the points have been approved in all phantoms. The approval criteria for IMRT plans recommended in most centers is 3% / 3mm with at least 95% of points approved, it can be concluded that, under these conditions, the IMRT plans with heterogeneity correction can be performed , however the quality control must be careful because the difficulty of the system to accurately predict the dose distribution in certain situations. (author)

  2. Biochemical and In-silico Studies on Pectin Methylesterase from G9 Variety of Musa acuminata for Delayed Ripening.

    Science.gov (United States)

    Verma, Charu; R K, Singh; Singh, Ram B; Mishra, Sanjay

    2015-01-01

    Ripening of fruit is a very important process but in some fruits early ripening leads to a great damage during long distance transportation. There are various biochemical changes taking place during the phase of ripening of fruit such as changes in respiration, aroma, flavor, ethylene production and activity of cell wall degrading enzymes. Some important cell wall degrading enzymes are Polygalacturonase (PG), Pectin methylesterase (PME), Pectin lyase, RGase. PME is known to act as a cell wall hydrolyzing enzyme, responsible for demethyl esterification of cell wall polygalacturonan. The present study includes the biochemical and molecular characterization of PME from Grand naine variety of Musa acuminata (banana). This study also deals with the in-silico study reflecting inhibition of PME activity in context to delayed ripening in banana. It mainly deals with the identification of a PME1 gene from Grand naine variety of banana. The expression of this gene is related with the process of ripening. The expression of PME1 gene was observed to be peaked on 3(rd) day in ethylene treated samples of banana but the activity in untreated samples called control was rather slow and then there was a sudden decrease in their activity in both treated as well as untreated samples. With the help of in-silico study, we observed that banana has maximum homology with carrot by using cross species analysis.The designed model has been reported to be of good quality on the basis of its verification and validation. The designed model was observed to be appropriate for docking. The information of binding sites of ligand provides new insights into the predictable functioning of relevant protein. PMID:25926894

  3. Cloning of an ADP-ribosylation factor gene from banana (Musa acuminata) and its expression patterns in postharvest ripening fruit.

    Science.gov (United States)

    Wang, Yuan; Wu, Jing; Xu, Bi-Yu; Liu, Ju-Hua; Zhang, Jian-Bin; Jia, Cai-Hong; Jin, Zhi-Qiang

    2010-08-15

    A full-length cDNA encoding an ADP-ribosylation factor (ARF) from banana (Musa acuminata) fruit was cloned and named MaArf. It contains an open reading frame encoding a 181-amino-acid polypeptide. Sequence analysis showed that MaArf shared high similarity with ARF of other plant species. The genomic sequence of MaArf was also obtained using polymerase chain reaction (PCR). Sequence analysis showed that MaArf was a split gene containing five exons and four introns in genomic DNA. Reverse-transcriptase PCR was used to analyze the spatial expression of MaArf. The results showed that MaArf was expressed in all the organs examined: root, rhizome, leaf, flower and fruit. Real-time quantitative PCR was used to explore expression patterns of MaArf in postharvest banana. There was differential expression of MaArf associated with ethylene biosynthesis. In naturally ripened banana, expression of MaArf was in accordance with ethylene biosynthesis. However, in 1-methylcyclopropene-treated banana, the expression of MaArf was inhibited and changed little. When treated with ethylene, MaArf expression in banana fruit significantly increased in accordance with ethylene biosynthesis; the peak of MaArf was 3 d after harvest, 11 d earlier than for naturally ripened banana fruits. These results suggest that MaArf is induced by ethylene in regulating postharvest banana ripening. Finally, subcellular localization assays showed the MaArf protein in the cytoplasm. PMID:20435371

  4. Influence of the Oil Pequi in the Growth Mycelial and Sporulation of Colletotrichum Musae and Growth Micelial Of Rhizopus sp. in vitro.

    Directory of Open Access Journals (Sweden)

    B. L. Mendes

    2013-07-01

    Full Text Available Abstract: The anthracnose caused by Colletotrichum musae and soft rot caused by Rhizopus sp are post-harvest diseases that influenced quality of exported fruits. To control these diseases fungicides are used, but in light of these waste products in the fruit and the environment, alternative control methods such as using biofungicides have been studied. The objective of this study was to evaluate the effect of pequi oil on mycelia growth and sporulation of Colletotrichum musae and mycelia growth of Rhizopus sp. in vitro. The oil was added in aliquots of 5, 10, 15, 20, 25, and 50L in the center of Petri dishes containing approximately 15mL of Potato-Dextrose-ar (PDA and distributed over the surface of the medium with Drigalskys handle. After 24 hours, a disc of 8mm diameter from mycelium of fungi was peaked to the center of the plates. As a control, were used Petri dishes containing only PDA. Five replicates were used for each aliquot. After analysis, it was concluded that the pequi oil is ineffective for controlling these pathogens because induced mycelium growth and sporulation.Key Words: Phytopathogens, alternative control, anthracnose, soft rot

  5. Development and application of sequence-tagged microsatellite site (STMS) markers in chickpea (Cicer arietinum), banana (Musa spp.) and their major pathogens, Ascochyta rabiei and Mycosphaerella fijiensis

    International Nuclear Information System (INIS)

    DNA markers of various kinds have found widespread application in many facets of plant breeding and plant pathogen control. Yet another marker type, sequence-tagged microsatellite (STMS) markers, provides the markers of choice for nearly every crop because of their co-dominant nature, reliability, ease of application and high polymorphic information content. We report here on the development of a whole set of STMS markers and the respective, selected primer sequences for two important crops, chickpea (Cicer arietinum L.) and banana (Musa acuminata), and for their most devastating fungal pathogens, Ascochyta rabiei and Mycosphaerella fijiensis, respectively. These markers were generated either by direct screening of size-selected genomic libraries with microsatellite-complementary oligonucleotides, or by enrichment of DNA fragments containing microsatellite sequences. A total of 69 markers for chickpea, 15 markers for M. acuminata, 19 markers for A rabiei and 11 markers for M. fijiensis, selected on the basis of their high information content and ease of use are presented here. These can be applied for mapping of the respective genomes, for various population studies, and cultivar and isolate identification. We further demonstrate that several of these markers can potentially be applied across species boundaries and thus could increase the marker repertoire also for other species of the genus Cicer, Musa and for Ascochyta-type pathogens of bean, and potentially also of lentil and pea. (author)

  6. The maize AAA-type protein SKD1 confers enhanced salt and drought stress tolerance in transgenic tobacco by interacting with Lyst-interacting protein 5.

    Directory of Open Access Journals (Sweden)

    Zongliang Xia

    Full Text Available ATPase associated with various cellular activities (AAA proteins are important regulators involved in diverse cellular functions. To date, the molecular mechanisms of AAA proteins involved in response to salt and drought stresses in plants are largely unknown. In this study, a putative SKD1 (suppressor of K(+ transport growth defect 1 ortholog from Zea mays (ZmSKD1, which encodes a putative AAA protein, was isolated. The transcript levels of ZmSKD1 were higher in aerial tissues and were markedly up-regulated by salt or drought stress. Over-expression of ZmSKD1 in tobacco plants enhanced their tolerances not only to salt but to drought. Moreover, reactive oxygen species accumulations in ZmSKD1 transgenic lines were relative less than those in wild-type plants during salt or PEG-induced water stress. The interaction between ZmSKD1 and NtLIP5 (Lyst-Interacting Protein 5 homolog from Nicotiana tabacum was confirmed by both yeast two-hybrid and immuno-precipitation assays; moreover, the α-helix-rich domain in the C-terminus of ZmSKD1 was identified to be required for its interaction with NtLIP5 using truncation mutations. Collectively, these data demonstrate that ZmSKD1could be involved in salt and drought stress responses and its over-expression enhances salt or drought stress tolerance possibly through interacting with LIP5 in tobacco. This study may facilitate our understandings of the biological roles of SKD1-mediated ESCRT pathway under stress conditions in higher plants and accelerate genetic improvement of crop plants tolerant to environmental stresses.

  7. A complex of mammalian Ufd1 and Npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways

    OpenAIRE

    Meyer, Hemmo H.; Shorter, James G.; Seemann, Joachim; Pappin, Darryl; Warren, Graham

    2000-01-01

    The AAA-ATPase, p97/Cdc48p, has been implicated in many different pathways ranging from membrane fusion to ubiquitin-dependent protein degradation. Binding of the p47 complex directs p97 to act in the post-mitotic fusion of Golgi membranes. We now describe another binding complex comprising mammalian Ufd1 and Npl4. Yeast Ufd1p is required for ubiquitin-dependent protein degradation whereas yeast Npl4p has been implicated in nuclear transport. In rat liver cytosol, Ufd1 and Npl4 form a binary ...

  8. Dosimetric accuracy and clinical quality of Acuros XB and AAA dose calculation algorithm for stereotactic and conventional lung volumetric modulated arc therapy plans

    International Nuclear Information System (INIS)

    The main aim of the current study was to assess the dosimetric accuracy and clinical quality of volumetric modulated arc therapy (VMAT) plans for stereotactic (stage I) and conventional (stage III) lung cancer treatments planned with Eclipse version 10.0 Anisotropic Analytical Algorithm (AAA) and Acuros XB (AXB) algorithm. The dosimetric impact of using AAA instead of AXB, and grid size 2.5 mm instead of 1.0 mm for VMAT treatment plans was evaluated. The clinical plan quality of AXB VMAT was assessed using 45 stage I and 73 stage III patients, and was compared with published results, planned with VMAT and hybrid-VMAT techniques. The dosimetric impact on near-minimum PTV dose (D98%) using AAA instead of AXB was large (underdose up to 12.3%) for stage I and very small (underdose up to 0.8%) for stage III lung treatments. There were no significant differences for dose volume histogram (DVH) values between grid sizes. The calculation time was significantly higher for AXB grid size 1.0 than 2.5 mm (p < 0.01). The clinical quality of the VMAT plans was at least comparable with clinical qualities given in literature of lung treatment plans with VMAT and hybrid-VMAT techniques. The average mean lung dose (MLD), lung V20Gy and V5Gy in this study were respectively 3.6 Gy, 4.1% and 15.7% for 45 stage I patients and 12.4 Gy, 19.3% and 46.6% for 73 stage III lung patients. The average contra-lateral lung dose V5Gy-cont was 35.6% for stage III patients. For stereotactic and conventional lung treatments, VMAT calculated with AXB grid size 2.5 mm resulted in accurate dose calculations. No hybrid technique was needed to obtain the dose constraints. AXB is recommended instead of AAA for avoiding serious overestimation of the minimum target doses compared to the actual delivered dose

  9. Analysis of non-TIR NBS-LRR resistance gene analogs in Musa acuminata Colla: Isolation, RFLP marker development, and physical mapping

    Directory of Open Access Journals (Sweden)

    Souza Manoel T

    2008-01-01

    Full Text Available Abstract Background Many commercial banana varieties lack sources of resistance to pests and diseases, as a consequence of sterility and narrow genetic background. Fertile wild relatives, by contrast, possess greater variability and represent potential sources of disease resistance genes (R-genes. The largest known family of plant R-genes encode proteins with nucleotide-binding site (NBS and C-terminal leucine-rich repeat (LRR domains. Conserved motifs in such genes in diverse plant species offer a means for isolation of candidate genes in banana which may be involved in plant defence. Results A computational strategy was developed for unbiased conserved motif discovery in NBS and LRR domains in R-genes and homologues in monocotyledonous plant species. Degenerate PCR primers targeting conserved motifs were tested on the wild cultivar Musa acuminata subsp. burmannicoides, var. Calcutta 4, which is resistant to a number of fungal pathogens and nematodes. One hundred and seventy four resistance gene analogs (RGAs were amplified and assembled into 52 contiguous sequences. Motifs present were typical of the non-TIR NBS-LRR RGA subfamily. A phylogenetic analysis of deduced amino-acid sequences for 33 RGAs with contiguous open reading frames (ORFs, together with RGAs from Arabidopsis thaliana and Oryza sativa, grouped most Musa RGAs within monocotyledon-specific clades. RFLP-RGA markers were developed, with 12 displaying distinct polymorphisms in parentals and F1 progeny of a diploid M. acuminata mapping population. Eighty eight BAC clones were identified in M. acuminata Calcutta 4, M. acuminata Grande Naine, and M. balbisiana Pisang Klutuk Wulung BAC libraries when hybridized to two RGA probes. Multiple copy RGAs were common within BAC clones, potentially representing variation reservoirs for evolution of new R-gene specificities. Conclusion This is the first large scale analysis of NBS-LRR RGAs in M. acuminata Calcutta 4. Contig sequences were

  10. INTEGRACIÓN DE INDUCCIÓN DE RESISTENCIA CON BACTERIAS QUITINOLÍTICAS EN EL CONTROL DE LA SIGATOKA NEGRA (Mycosphaerella fijiensis Morelet EN BANANO INTEGRATION OF INDUCED RESISTANCE WITH CHITINOLYTIC BACTERIA IN BLACK SIGATOKA CONTROL (Mycosphaerella fijiensis Morelet IN BANANA

    Directory of Open Access Journals (Sweden)

    Catalina María Zuluaga Amaya

    2007-12-01

    Full Text Available Se evaluó en condiciones de campo en la zona bananera de Urabá-Colombia, el efecto de inductores de resistencia y bacterias quitinolíticas sobre el desarrollo de la Sigatoka Negra en plantas de banano del cultivar Gran Enano. Como inductores de resistencia se utilizaron acibenzolar-s-metil (ASM (20 cm³ · ha-1 i.a. y ácido salicílico (AS (20 cm³ · ha-1 i.a cada uno en rotación y en mezcla con fungicidas utilizados en el control convencional de la enfermedad; se evaluó también la mezcla de estos inductores con bacterias quitinolíticas aisladas de la filosfera de banano; así como el efecto del fungicida biológico Biofun® ( 1,5 L · ha-1 de p.c. en rotación con fungicidas y en mezcla con ASM. La aplicación de ASM en rotación y en mezcla con fungicidas convencionales presentó un control de la enfermedad hasta un 50% mayor al obtenido con el control convencional, evidenciado esto en los valores más bajos del área bajo la curva del índice de severidad de la enfermedad (IS y más altos de hojas sanas (HS al momento de la floración. Los tratamientos, que incluyeron AS en rotación y mezcla con fungicidas convencionales, al igual que el producto Biofun® en rotación y en mezcla con ASM, mostraron un control de la enfermedad estadísticamente igual al obtenido con el control convencional, lo cual reveló una reducción de 46 - 100% de la cantidad de fungicidas convencionales usados en el control de la Sigatoka Negra.The effect of resistance elicitors and chitinolytic bacteria was evaluated on Black Sigatoka control in banana plants, in field conditions in Urabá-Colombia. Acibenzolar-s-metil (ASM (20 cm³ · ha- 1 a.i. and salicilyc acid (SA (20 cm³ a.i.ha-1 were used in rotation and mixture with conventional fungicides, and in mixture with chitinolytic bacteria isolated from banana phyllosphere. Biological fungicide Biofun® ( 1,5 L · ha-1 de p.c. was also tested in rotation and mixture with ASM. When fungicides were in rotation

  11. AAA安全管理系统在数字图书馆中的应用%Application of AAA Secure Management Information System to Digital Library

    Institute of Scientific and Technical Information of China (English)

    雷向康

    2009-01-01

    针对当前数字图书馆网络系统设备中普遍存在的安全隐患问题,提出在各网络设备中使用基于AAA(认证、授权、记账)的方法,在管理和控制端,使用一套Cisco Secure ACS(思科安全访问控制服务器)软件, 用TACACS+或RADIUS协议与ACS进行通信认证.AAA安全管理系统部署后,成功地解决了这一难题,实验证明本方法行之有效.%In order to overcome the common obstacle of secure and peril problems of the switchs in digital library networks, this paper develops the method based on AAA(Authentication、Authorization、Accounting) in client switchs of networks and Cisco Secure ACS(Access Control Server)in management server,by communication between switchs and ACS through protocol of TACACS+ or RADIUS.The method is tested successfully and proved effectively in our ditital library.

  12. Respuesta Glucémica e Insulínica de Pacientes con Diabetes Tipo 2 al consumo de Sopa de Calabaza Criolla (Cucúrbita Pepo L. Enriquecida con Almidón de Banano Glycemic and Insulinic Response of Patients with Type 2 diabetes to the consumption of Pumpkin Soup Creole (Cucúrbita Pepo L. Enriched with Banana Starch

    Directory of Open Access Journals (Sweden)

    Angel E Torres-Zapata

    2012-01-01

    Full Text Available El objetivo de este trabajo fue determinar el índice glucémico y la respuesta insulínica de una sopa conteniendo almidón nativo de banano. Los efectos benéficos del consumo de almidón resistente sobre el control glucémico han sido reportados previamente, pero estos parámetros no han sido suficientemente estudiados. Las determinaciones se realizaron en 12 sujetos sanos y 12 con diabetes tipo 2 y el índice glucémico de la sopa fue de 31.79. Las áreas bajo las curvas de glucosa e insulina fueron menores en los grupos con la sopa en comparación con el alimento de referencia (PThe aim of this study was to determine the glycemic index value and the insulinic response of a soup containing native banana starch. The beneficial effects of resistant starch in dietary supplementation on glycemic control have been reported previously, but these parameters have not been sufficiently studied. Determinations were carried out in two groups: one of 12 healthy subjects and other with 12 type 2 diabetes. The estimated glycemic index value of the soup was 31.79. The total glucose and insulin areas under the curves were reduced in comparison with the reference food (P <0.01. The enriched soup with native banana starch might help in preventing type 2 diabetes prevalence in health subjects with high risk. Also, this product can be used to decrease complications of people with diabetes.

  13. Multicenter Orthopaedic Outcome Network Early Anti-inflammatory Treatment in Patients with Acute ACL Tear” (MOON-AAA) Clinical Trial

    Science.gov (United States)

    Lattermann, Christian; Proffitt, Mary; Huston, Laura J.; Gammon, Lee; Johnson, Darren L.; Kraus, Virginia B.; Spindler, Kurt P.

    2016-01-01

    Objectives: We present the early results from the “Multicenter Orthopaedic Outcome Network Early Anti-inflammatory Treatment in Patients with Acute ACL Tear and Painful Effusions” (MOON-AAA) clinical trial (figure 1). This trial allows for a well controlled prospective cohort of patients with isolated ACL injury at risk for OA. We compared the effect of a single versus a repeated dosage of Kenalog within the first two weeks after ACL injury and its effect on chondral degradation in the first 4 weeks prior to surgical reconstruction of the ACL. Methods: 49 patients with isolated ACL tears were enrolled. Knee joints were aspirated and patients received an injection with 40 mg Kenalog either within 4 days, 10 days, both time points or not at all (saline injection control). Serum, synovial fluid and urine were collected at 3 time points. Permutated block randomization, triple blinding, independent monitoring and standardized x-ray was performed to comply with GCP standards. Patient reported outcomes were collected at 6 time points up to 6 months post-ACL reconstruction(IKDC, KOOS and Marx activity level). A standardized synovial fluid biomarker panel was analyzed according to OARSI guidelines. Statistical analysis were performed using SAS mixed models analysis. Results: Serum analysis shows significant change after injury. Chondrodegradatory markers such as CTX-II, MMP-1 and MMP-3 as well as COMP indicate a progressive destruction of chondral matrix and collagen breakdown . There is a dramatic (250%) increase of CTX-II in the first 4 weeks. Matrix proteins such as MMP-1 and 3 as well as COMP show an initial increase and then a steep decline (see figure 1). Inflammatory markers (IL-1 alpha, IL-1beta, IRAP) show a decline from the time of injury. IL-1 alpha, however shows a dramatic uptake after week 2. This longitudinal data confirms a dramatic onset of early osteoarthritic biomarker profiles immediately after ACL injury as measured in synovial fluid

  14. Caracterización de harina y almidón obtenidos a partir de plátano guineo AAAea (Musa sapientum L.)

    OpenAIRE

    Juan Carlos Lucas; Víctor Dumar Quintero; Carlos Andrés Cárdenas Valencia

    2013-01-01

    En el estudio se caracterizaron las propiedades química, térmica, funcional y morfológica de la harina y el almidón de guineo AAAea (Musa sapientum L.). Los resultados mostraron un rendimiento de 33.33% para harina y 3.61% para almidón. El porcentaje de proteína en la harina fue de 5.43% y en almidón de 2.17%. La fibra soluble en harina fue de 19.85% y de grasa en el almidón de 4.11 %. El análisis térmico de la harina mostró que la etapa de descomposición varía desde 141.33 °C hasta 388.30 °C...

  15. BESTECİ MUSA MİRZAYEV VE ONUN TÜRKİYE AZERBAYCAN SANAT İLİŞKİLERİNDEKİ YERİ

    OpenAIRE

    Hasanova, Şehla

    2010-01-01

    ÖZETAzerbaycan'ın bağımsızlığını kazanması ile birlikte Türkiye-Azerbaycan sosyokültürelilişkilerinde önemli bir atılım başlamıştır. Bu süreçle pek çok sanatçı ilebirlikte Besteci Musa Mirzayev 'in sanatı ve çalışmaları da önemli rol oynamıştır.Onun sanat ve eserleri Türkiye'de, Türkiye müzik kültürü unsurları da onlarınvasıtası ile Azerbaycan 'da seslenme imkânı bulmuştur.Anahtar Sözcü...

  16. Histology of Callogenesis in Diploid Bananas (Musa acuminata, AA Group �Kluai Sa� and �Kluai Leb Mu Nang�

    Directory of Open Access Journals (Sweden)

    Kamnoon KANCHANAPOOM

    2012-02-01

    Full Text Available Yellow compact calluses were induced from in vitro-grown shoot tips of diploid bananas (Musa acuminata, AA group �Kluai Sa� and �Kluai Leb Mu Nang� on a modified Murashige and Skoog (MS medium containing 100 mg/L malt extract, 50 mg/L proline, 50 mg/L cysteine, 100 mg/L glutamine, 1 mg/L biotin, 7 mg/L Dicamba and 2 mg/L TDZ. Green shoot buds were induced after transfer of the yellow compact calluses to the same MS medium but supplemented with 1 mg/L NAA and 3 mg/L BA and plant regeneration was achieved through organogenesis in callus cultures. Regenerated shoots were rooted on MS medium containing 0.2% activated charcoal but without plant growth regulators. Histological analysis revealed that calluses originated from small dense cells with well stained cytoplasm and nucleus typical of meristematic cells.

  17. Efectos de dos sistemas de producción en el contenido de minerales en el fruto de Musa AAB Simmonds

    OpenAIRE

    Guerra Hernández Katty Julia; Mizger Pacheco Marcela Lucía; Barrera Violeth José Luis

    2011-01-01

    Los sistemas de producción orgánico y/o ecológico tienen como objetivo garantizar la sostenibilidad y la renovación de base natural, mediante la limitación del uso de productos de síntesis química para favorecer el ambiente y la salud humana. Teniendo en cuenta estas características, se estudió el efecto de los sistemas de producción orgánico y convencional sobre el contenido de nutrientes minerales del fruto del plátano Hartón (Musa AAB Simmonds), durante el proceso de m...

  18. Chemical Composition, Nutritive and Energetic Value of Plantain (Musa ssp. Hybrids CRBP 14, CRBP 39, FHIA 17, FHIA 21and Orishele Variety

    Directory of Open Access Journals (Sweden)

    Coulibaly, S.

    2007-01-01

    Full Text Available Chemical characteristics as well as contents in main components such as dry matter, sugars, lipids proteins and some minerals were determined in four banana hybrids CRBP 14, CRBP 39, FHIA 17, FHIA 21 and Orishele variety (used as control. Titrable acidity, pH, and calorific energy of these various cultivars of banana (Musa ssp. were also determined. The results indicated that Orishele variety had the highest content of dry matter, total carbohydrate and potassium. On the other hand, CRBP 39 hybrid which had the highest total acidity was the richest in total sugars and was the most energetic. Concerning FHIA 17, it contained the highest amount of proteins, lipids, magnesium, calcium and iron.

  19. Acclimatation de vitroplants de bananier Musa sp. en culture hydroponique: impact de différentes concentrations en cuivre sur la croissance des vitroplants

    Directory of Open Access Journals (Sweden)

    Mazinga, MK.

    2013-01-01

    Full Text Available Acclimatation of Banana's Vitroplants (Musa sp. in Hydroponic Culture: Effects of Different Concentrations Copper on Growth of Vitroplants. Constraints due to copper and consequences of its accumulation in acclimatized banana in vitroplants have been studied in hydroponic culture. 0-100- 500-1000 ppm copper was added to the nutrient medium in hydroponic culture. At 1000 ppm, copper was accumulated in the roots but not in the aerial parts. Surprisingly, biomass of shoots and roots was augmented significantly at this concentration (with leaves as an exception. Plant height was reduced strongly even at 100 ppm CuSO4, although the copper content in shoots and leaves was very low.

  20. Arilsulfatasa en la rizosfera de plátano, musa aab y relación con crecimiento, desarrollo y produccion

    OpenAIRE

    Bolaños B, Martha M; Sanchez de Prager, Marina; Rao, Idupulapati M.

    2006-01-01

    En la zona central cafetera de Colombia, en un suelo Pachic Melanudands medial isotérmico, se midió la actividad de arilsulfatasa en rizosfera de plátano Musa AAB, en tres sistemas de manejo agronómico (químico o convencional, tradicional y ecológico), cuatro profundidades (0-5, 5-10, 10-20 y 20-30 cm) y tres edades de cultivo (6 meses – diferenciación floral, 12 meses – floración, 18 meses - cosecha). La mayor actividad (60%) se encontró en los primeros 10 cm., y disminuyó a medida que se ...

  1. Pollination Ecology of a Pioneer Species: Musa itinerans (Musaceae) in Xishuangbanna, South Yunnan, China%西双版纳先锋植物野芭蕉的传粉生态学研究

    Institute of Scientific and Technical Information of China (English)

    刘爱忠; 李德铢; 王红

    2001-01-01

    Pollination ecology of Musa itinerans Cheesman (Musaceae), apioneer species in the tropical rain forest, was explored in Xishuangbanna, South Yunnan, China. This research involved flowering phenology, nectar production, visitation patterns of pollinators and bagging experiments. It was found that (1) flowering of M. itinerans occurred a whole year round with a peak at the early dry season (Nov.) and the daily flowering pattern had two obvious peaks in the early morning and in the late evening, respectively; (2) nectar production occurred at two obvious peaks, during the day and in the night-time (from 8 am to 12 pm, and from 8 pm to 12 am, respectively), which allowed the two different foragers to visit at specific times; and (3) long-tongue fruit bats (Macroglossus sobrinus) and sunbirds (Arachnothera longirostris) were both effective pollinators of Musa itinerans.

  2. Geometrical determinations of IMRT photon pencil-beam path in radiotherapy wedges and limit divergence angle with the Anisotropic Analytic Algorithm (AAA

    Directory of Open Access Journals (Sweden)

    Francisco Casesnoves

    2014-08-01

    Full Text Available Purpose: Static wedge filters (WF are commonly used in radiation therapy, forward and/or inverse planning. We calculated the exact 2D/3D geometrical pathway of the photon-beam through the usual alloy WF, in order to get a better dose related to the beam intensity attenuation factor(s, after the beam has passed through the WF. The objective was to provide general formulation into the Anisotropic Analytical Algorithm (AAA model coordinates system (depending on collimator/wedge angles that also can be applied to other models. Additionally, second purpose of this study was to develop integral formulation for 3D wedge exponential factor with statistical approximations, with introduction for the limit angle/conformal wedge.Methods: The radiotherapy model used to develop this mathematical task is the classical superposition-convolution algorithm, AAA (developed by Ulmer and Harder. We worked with optimal geometrical approximations to make the computational IMRT calculations quicker/reduce the planning-system time. Analytic geometry/computational-techniques to carry out simulations (for standard wedges are detailed/developed sharply. Integral developments/integral-statistical approximations are explained. Beam-divergence limit Angle for optimal wedge filtration formulas is calculated/sketched, with geometrical approximations. Fundamental trigonometry is used for this purpose.Results: Extent simulation tables for WF of 15º, 30º, 45º, and 60º are shown with errors. As a result, it is possible to determine the best individual treatment dose distribution for each patient. We presented these basic simulations/numerical examples for standard manufacturing WF of straight sloping surface, to check the accuracy/errors of the calculations. Simulations results give low RMS/Relative Error values (formulated for WF of 15º, 30º, 45º, and 60º.Conclusion: We obtained a series of formulas of analytic geometry for WF that can be applied for any particular dose

  3. Comparison of doses and NTCP to risk organs with enhanced inspiration gating and free breathing for left-sided breast cancer radiotherapy using the AAA algorithm

    International Nuclear Information System (INIS)

    The purpose of this study was to investigate the potential dose reduction to the heart, left anterior descending (LAD) coronary artery and the ipsilateral lung for patients treated with tangential and locoregional radiotherapy for left-sided breast cancer with enhanced inspiration gating (EIG) compared to free breathing (FB) using the AAA algorithm. The radiobiological implication of such dose sparing was also investigated. Thirty-two patients, who received tangential or locoregional adjuvant radiotherapy with EIG for left-sided breast cancer, were retrospectively enrolled in this study. Each patient was CT-scanned during FB and EIG. Similar treatment plans, with comparable target coverage, were created in the two CT-sets using the AAA algorithm. Further, the probability of radiation induced cardiac mortality and pneumonitis were calculated using NTCP models. For tangential treatment, the median V25Gy for the heart and LAD was decreased for EIG from 2.2% to 0.2% and 40.2% to 0.1% (p < 0.001), respectively, whereas there was no significant difference in V20Gy for the ipsilateral lung (p = 0.109). For locoregional treatment, the median V25Gy for the heart and LAD was decreased for EIG from 3.3% to 0.2% and 51.4% to 5.1% (p < 0.001), respectively, and the median ipsilateral lung V20Gy decreased from 27.0% for FB to 21.5% (p = 0.020) for EIG. The median excess cardiac mortality probability decreased from 0.49% for FB to 0.02% for EIG (p < 0.001) for tangential treatment and from 0.75% to 0.02% (p < 0.001) for locoregional treatment. There was no significant difference in risk of radiation pneumonitis for tangential treatment (p = 0.179) whereas it decreased for locoregional treatment from 6.82% for FB to 3.17% for EIG (p = 0.004). In this study the AAA algorithm was used for dose calculation to the heart, LAD and left lung when comparing the EIG and FB techniques for tangential and locoregional radiotherapy of breast cancer patients. The results support the dose and

  4. Production potentielle de bioéthanol, de biométhane et de pellets à partir des déchets de biomasse lignocellulosique du bananier (Musa spp.) au Cameroun

    OpenAIRE

    Kamdem, I.; TOMEKPE, K.; Thonart, P.

    2011-01-01

    Potential production of bioethanol, biomethane and wood pellets from lignocellulosic biomass wastes of the banana plant (Musa spp.) in Cameroon. Like most African countries who are producers and exporters of banana, Cameroon is facing a major energy deficit. Yet, the country is generating annually about 4,500,000 tons of fresh banana plant lignocellulosic waste biomass matter equivalent to 402,750 tons of dry matter. The dry matter contained about 80,57% organic matter which are not exploited...

  5. Sugar and Amino Acid Contents of Fruit and Foliar Tissues from Two Cultivars of Plantain (Musa paradisiaca) Susceptible and Resistant to Cigar-end Rot Disease Caused by Verticillium theobromae

    OpenAIRE

    C.L. Igeleke; D.K.G. Ayanru

    2006-01-01

    Floral and fruit tissues of two cultivars of plantain (Musa paradisiacal L.), P100-F (susceptible) and P200-1 (resistant to cigar-end rot disease), grown side by side in a field plot in Benin City, Nigeria, were analysed for soluble and tissue-bound amino acids, total carbohydrate and crude protein using paper chromatography and micro Kjeldahl methods. Diminished carbohydrate and protein concentrations and intensity spotting of alanine and leucine were found in fruit tissue digest of the susc...

  6. TU-C-BRE-05: Clinical Implications of AAA Commissioning Errors and Ability of Common Commissioning ' Credentialing Procedures to Detect Them

    International Nuclear Information System (INIS)

    Purpose: To test the ability of the TG-119 commissioning process and RPC credentialing to detect errors in the commissioning process for a commercial Treatment Planning System (TPS). Methods: We introduced commissioning errors into the commissioning process for the Anisotropic Analytical Algorithm (AAA) within the Eclipse TPS. We included errors in Dosimetric Leaf Gap (DLG), electron contamination, flattening filter material, and beam profile measurement with an inappropriately large farmer chamber (simulated using sliding window smoothing of profiles). We then evaluated the clinical impact of these errors on clinical intensity modulated radiation therapy (IMRT) plans (head and neck, low and intermediate risk prostate, mesothelioma, and scalp) by looking at PTV D99, and mean and max OAR dose. Finally, for errors with substantial clinical impact we determined sensitivity of the RPC IMRT film analysis at the midpoint between PTV and OAR using a 4mm distance to agreement metric, and of a 7% TLD dose comparison. We also determined sensitivity of the 3 dose planes of the TG-119 C-shape IMRT phantom using gamma criteria of 3% 3mm. Results: The largest clinical impact came from large changes in the DLG with a change of 1mm resulting in up to a 5% change in the primary PTV D99. This resulted in a discrepancy in the RPC TLDs in the PTVs and OARs of 7.1% and 13.6% respectively, which would have resulted in detection. While use of incorrect flattening filter caused only subtle errors (<1%) in clinical plans, the effect was most pronounced for the RPC TLDs in the OARs (>6%). Conclusion: The AAA commissioning process within the Eclipse TPS is surprisingly robust to user error. When errors do occur, the RPC and TG-119 commissioning credentialing criteria are effective at detecting them; however OAR TLDs are the most sensitive despite the RPC currently excluding them from analysis

  7. TU-C-BRE-05: Clinical Implications of AAA Commissioning Errors and Ability of Common Commissioning ' Credentialing Procedures to Detect Them

    Energy Technology Data Exchange (ETDEWEB)

    McVicker, A; Oldham, M; Yin, F; Adamson, J [Duke University Medical Center, Durham, NC (United States)

    2014-06-15

    Purpose: To test the ability of the TG-119 commissioning process and RPC credentialing to detect errors in the commissioning process for a commercial Treatment Planning System (TPS). Methods: We introduced commissioning errors into the commissioning process for the Anisotropic Analytical Algorithm (AAA) within the Eclipse TPS. We included errors in Dosimetric Leaf Gap (DLG), electron contamination, flattening filter material, and beam profile measurement with an inappropriately large farmer chamber (simulated using sliding window smoothing of profiles). We then evaluated the clinical impact of these errors on clinical intensity modulated radiation therapy (IMRT) plans (head and neck, low and intermediate risk prostate, mesothelioma, and scalp) by looking at PTV D99, and mean and max OAR dose. Finally, for errors with substantial clinical impact we determined sensitivity of the RPC IMRT film analysis at the midpoint between PTV and OAR using a 4mm distance to agreement metric, and of a 7% TLD dose comparison. We also determined sensitivity of the 3 dose planes of the TG-119 C-shape IMRT phantom using gamma criteria of 3% 3mm. Results: The largest clinical impact came from large changes in the DLG with a change of 1mm resulting in up to a 5% change in the primary PTV D99. This resulted in a discrepancy in the RPC TLDs in the PTVs and OARs of 7.1% and 13.6% respectively, which would have resulted in detection. While use of incorrect flattening filter caused only subtle errors (<1%) in clinical plans, the effect was most pronounced for the RPC TLDs in the OARs (>6%). Conclusion: The AAA commissioning process within the Eclipse TPS is surprisingly robust to user error. When errors do occur, the RPC and TG-119 commissioning credentialing criteria are effective at detecting them; however OAR TLDs are the most sensitive despite the RPC currently excluding them from analysis.

  8. Study on Computerized Treatment Plan of Field-in-Field Intensity Modulated Radiation Therapy and Conventional Radiation Therapy according to PBC Algorithm and AAA on Breast Cancer Tangential Beam

    International Nuclear Information System (INIS)

    Anisotropic Analytical Algorithm (AAA) provides more accurate dose calculation regarding impact on scatter and tissue inhomogeneity in comparison to Pencil Beam Convolution (PBC) algorithm. This study tries to analyze the difference of dose distribution according to PBC algorithm and dose calculation algorithm of AAA on breast cancer tangential plan. Computerized medical care plan using Eclipse treatment planning system (version 8.9, VARIAN, USA) has been established for the 10 breast cancer patients using 6 MV energy of Linac (CL-6EX, VARIAN, USA). After treatment plan of Conventional Radiation Therapy plan (Conventional plan) and Field-in-Field Intensity Modulated Radiation Therapy plan (FiF plan) using PBC algorithm has been established, MU has been fixed, implemented dose calculation after changing it to AAA, and compared and analyzed treatment plan using Dose Volume Histogram (DVH). Firstly, as a result of evaluating PBC algorithm of Conventional plan and the difference according to AAA, the average difference of CI value on target volume has been highly estimated by 0.295 on PBC algorithm and as a result of evaluating dose of lung, V47 Gy and has been highly evaluated by 5.83% and 4.04% each, Mean dose, V20, V5, V3 Gy has been highly evaluated 0.6%, 0.29%, 6.35%, 10.23% each on AAA. Secondly, in case of FiF plan, the average difference of CI value on target volume has been highly evaluated on PBC algorithm by 0.165, and dose on ipsilateral lung, V47, V45 Gy, Mean dose has been highly evaluated 6.17%, 3.80%, 0.15% each on PBC algorithm, V20, V5, V3 Gy has been highly evaluated 0.14%, 4.07%, 4.35% each on AAA. When calculating with AAA on breast cancer tangential plan, compared to PBC algorithm, Conformity on target volume of Conventional plan, FiF plan has been less evaluated by 0.295, 0.165 each. For the reason that dose of high dose region of ipsilateral lung has been showed little amount, and dose of low dose region has been showed much amount, features

  9. Evaluación de la degradación ambiental de materiales termoplásticos empleados en labores agrícolas en el cultivo de banano en Colombia Environmental degradation of thermoplastic materials used in banana agricultural labors in Colombia

    Directory of Open Access Journals (Sweden)

    José L. Mejía

    2007-09-01

    Full Text Available La producción de banano es una dinámica actividad económica en Colombia, sin embargo durante las labores agrícolas, las plantas deben ser unidas unas a otras a través de cuerdas. Esta prácticas contribuye a solventar el peso de los frutos antes de la cosecha. Cuerdas de polipropileno son utilizadas para este fin. Sin embargo, esta práctica genera problemas ambientales en las regiones de cultivo, haciendo necesario identificar materiales biodegradables alternativos. En este estudio, muestras de polipropileno, mezclas de polipropileno con almidón, así como un biopolímero conocido como Mater-Bi y éste combinado con PP fueron analizados. Su estabilidad fue evaluada al ser expuestas a las condiciones medioambientales de la región tropical de Urabá (Colombia. Los cambios en la estructura de los materiales expuestos fueron analizados utilizando calorimetría diferencial de barrido (DSC y espectroscopía infrarroja FTIR. Pequeñas diferencias en el comportamiento mecánico y físico de las muestras expuestas fueron observadas, y pese a que la adición tanto del almidón como del biopolímero reducen el comportamiento a tracción del polipropileno, estas mezclas pueden ser útiles para esta aplicación.Banana fruit production is a dynamic economical sector in Colombia. However during the agricultural labor, banana plants need to be tied to each other using strings. This practice helps to support the fruit weight before the harvesting. Polypropylene strings are used in this application. This practice is becoming an environmental issue in cultivation areas, and it is necessary to identify biodegradable alternative materials. In this study, samples of polypropylene, polypropylene/starch blend and a commercial biopolymer, referred to as Mater-Bi and PP/ Mater-Bi, were analyzed. The stability of these materials in tropical regions such as Uraba (Colombia was evaluated. The changes in the material structure and properties were analyzed by

  10. 基于cpSSR分子标记的香蕉种质资源分类%The classification of Musa species using chloroplast SSR primers

    Institute of Scientific and Technical Information of China (English)

    李博; 冯慧敏; 王静毅; 童和林; 陈友; 武耀廷

    2011-01-01

    Choroplast genome was involved in the development of cpSSR primer pairs in Musa and the transferability of cpSSR markers from other plants across Musa wild species was observed. A set of 16 polymorphic cpSSR primers were selected to classify 42 related species/subspecies. A total of 86 polymorphic bands with an average of 5.5 bands (range 2 to 8) were identified. The PIC values ranged from 0.188 9 to 0.780 5. The UPGMA dendrogram divided the banana accessions into three main groups based on the similarity coefficient 0.76. Group Ⅰ included all the subspecies of M. acuminata and all the cultivated banana varieties. All the materials of M. balbisiana formed Group Ⅱ. Group m included all the wild materials except the species/subspecies of M. acuminata and M. balbisiana. The results showed that cpSSR marker was useful to distinguish the related species of Musa. The cluster analysis of cpSSR in present study revealed that the wild species/subspecies could be well grouped according to the geographical origin and but not to the morphological characters.%应用cpSSR分子标记来研究香蕉种质资源分类,用筛选到的16对多态性引物对42份香蕉材料进行扩增.共检测出86个多态性条带,每对引物产生的多态性条带为2~8,平均为5.5,多态信息含量为0.1889~0.7805。在相似系数为0.76时,供试的42份香蕉材料分成3大类群:类群Ⅰ为M.acuminata的全部亚种和供试的所有栽培蕉品种;类群Ⅱ为供试的全部M.balbisiana材料;类群Ⅲ为除了M.acuminata和M.balbisiana外其他全部供试的野生蕉材料。结果表明基于cpSSR分子标记的香蕉种质资源的分类在种间具有较好的区分能力,但是cpSSR分类表现出较大的地理分布的相关性,而与形态特征关系不密切。

  11. Hairpin loops consisting of single adenine residues closed by sheared A.A and G.G pairs formed by the DNA triplets AAA and GAG: solution structure of the d(GTACAAAGTAC) hairpin.

    Science.gov (United States)

    Chou, S H; Zhu, L; Gao, Z; Cheng, J W; Reid, B R

    1996-12-20

    The DNA undecamers GTACAAAGTAC (AAA 11-mer) and GTACGAGGTAC (GAG 11-mer) have been studied in solution by high-resolution NMR spectroscopy. Both duplexes form stable hairpins containing single deoxyadenosine loops and stems containing five base-pairs that are closed at the loop end by sheared AxA and GxC pairs, respectively. These molecules thus contain new AAA and GAG loop turn motifs. All protons, including the chiral H5'/H5" protons of the loop residues, were assigned using NOESY, DQF-COSY and heteronuclear 1H-31P COSY experiments. The backbone torsion angles were constrained using experimental data from NOE crosspeaks, three-bond 1H-1H coupling constants and four-bond 1H-31P coupling constants and four-bond 1H-31P coupling constants. The AAA and GAG 11-mers form similar structures in solution. The detailed structure of the AAA 11-mer was determined by the combined use of NMR, distance geometry and energy minimization methods. This structure exhibits good stacking of the loop adenosine base on the closing 5Ax7A sheared pair, with the 6A base stacking on the 5A base and the 6A deoxyribose stacking with the 7A base. All sugars in the AAA 11-mer hairpin adopt the typical DNA C2'-endo conformation and a sharp backbone turn occurs between residues 6A and 7A. This loop turn is brought about mainly by a change in the backbone phosphate torsion angles from zeta(g-) alpha(g-) to zeta(g+) alphat(g+) at the turn. The gamma torsion angle of residue 7A in the closing sheared pair also changes from gauche+ to trans. In Pu1NPu2 loop turns of the GCA, AAA and GAG types, the chemical shift of the H4' proton of the loop deoxyribose depends on the nature of Pu2; this reflects the stacking of the loop sugar on the Pu2 base and the different ring current effects of A or G in this position. PMID:9000625

  12. Hot water treatments delay cold-induced banana peel blackening

    NARCIS (Netherlands)

    Promyou, S.; Ketsa, S.; Doorn, van W.G.

    2008-01-01

    Banana fruit of cv. Gros Michel (Musa acuminata, AAA Group, locally called cv. Hom Thong) and cv. Namwa (Musa x paradisiaca, ABB Group) were immersed for 5, 10 and 15 min in water at 42 degrees C, or in water at 25 degrees C (control), and were then stored at 4 degrees C. Hot water treatment for 15

  13. AAA服务器在流媒体系统中的应用研究%Research on Application of AAA Server in the Streaming Media System.

    Institute of Scientific and Technical Information of China (English)

    李远英

    2013-01-01

    AAA refers to the athentication (authentication), authorization (Licensing), accounting (accounting). Since the birth of the network, authentication, authorization and accounting system have become the basis of the operation. The use of the resources on network, requires authentication, authorization and accounting to management. Confirm the identity of the user, according to the application when the user service category, system can grant customer permission. Finally, in the use of system resources to the user, need to have the appropriate equipment to statistical occupancy of resource users, according to the user to collect the corresponding costs[1]. The main work of the paper is to complete the AAA server design and in the RADIUS protocol, the RADIUS authentication/ authorized server structure design, through the analysis of the structure and further improvement, the test results show that the design and implementation of streaming media server structure can meet the real time management system, making the system accurate billing, carried on the test to the system and reached the purpose of this study.%  AAA指的是athentication(认证)、authorization(授权)、accounting(计费)。自网络诞生以来,认证、授权和计费体制就成为其运营的基础。网络中各类资源的使用,需要认证、授权和计费进行管理。在确认用户身份后,根据用户开户时所申请的服务类别,系统可以授予客户相应的权限。最后在用户使用系统资源时,需要有相应的设备来统计用户对资源的占用情况,据此向用户收取相应的费用[1]。论文的主要工作是完成AAA服务器的设计和在RADIUS协议下,设计RADIUS认证/授服务器结构,通过对这种结构的分析及进一步的改进,其测试结果表明服务器结构的设计和实现能够满足流媒体系统实时管理,使得系统计费准确,对系统进行了测试,基本达到研究目的。

  14. The CoxD protein, a novel AAA+ ATPase involved in metal cluster assembly: hydrolysis of nucleotide-triphosphates and oligomerization.

    Directory of Open Access Journals (Sweden)

    Tobias Maisel

    Full Text Available CoxD of the α-proteobacterium Oligotropha carboxidovorans is a membrane protein which is involved in the posttranslational biosynthesis of the [CuSMoO₂] cluster in the active site of the enzyme CO dehydrogenase. The bacteria synthesize CoxD only in the presence of CO. Recombinant CoxD produced in E. coli K38 pGP1-2/pETMW2 appeared in inclusion bodies from where it was solubilized by urea and refolded by stepwise dilution. Circular dichroism spectroscopy revealed the presence of secondary structural elements in refolded CoxD. CoxD is a P-loop ATPase of the AAA-protein family. Refolded CoxD catalyzed the hydrolysis of MgATP yielding MgADP and inorganic phosphate at a 1∶1∶1 molar ratio. The reaction was inhibited by the slow hydrolysable MgATP-γ-S. GTPase activity of CoxD did not exceed 2% of the ATPase activity. Employing different methods (non linear regression, Hanes and Woolf, Lineweaver-Burk, preparations of CoxD revealed a mean K(M value of 0.69±0.14 mM ATP and an apparent V(max value of 19.3±2.3 nmol ATP hydrolyzed min⁻¹ mg⁻¹. Sucrose density gradient centrifugation and gel filtration showed that refolded CoxD can exist in various multimeric states (2-mer, 4-mer or 6-mer, preferentially as hexamer or dimer. Within weeks the hexamer dissociates into the dimer, a process which can be reversed by MgATP or MgATP-γ-S within hours. Only the hexamers and the dimers exhibited MgATPase activity. Transmission electron microscopy of negatively stained CoxD preparations revealed distinct particles within a size range of 10-16 nm, which further corroborates the oligomeric organization. The 3D structure of CoxD was modeled with the 3D structure of BchI from Rhodobacter capsulatus as template. It has the key elements of an AAA+ domain in the same arrangement and at same positions as in BchI and displays the characteristic inserts of the PS-II-insert clade. Possible functions of CoxD in [CuSMoO₂] cluster assembly are discussed.

  15. Determinación del tipo de planta adecuado para el monitoreo de poblaciones de Fito nematodos en plátano (Musa AAB (ING

    Directory of Open Access Journals (Sweden)

    Mario Araya

    2016-03-01

    Full Text Available On two plantations of first harvest of Musa AAB each consisting of separate lots of False Horn Plantain (Semi-Giant and Dwarf nematode populations were determined in plants with one to three days flowering and in their respective succeeding suckers. The objective was to determine if there is a relationship between the nematode population in mother plants and their succeeding suckers. Sixty and twenty plants of each cultivar were evaluated in Pococí (70 masl and Matina (40 masl locations, respectively. Nematodes were extracted by maceration of the roots, pouring the suspension through a series of 0.5/0.150/0.038 mm (N° 30/100/400 mesh sieves nested together with the 0.5 mm on top. Populations levels were expressed per 100 g of roots. The results showed no linear relationship between the nematode populations of the flowering plants and their succeeding sucker. Although the differences were not statistically significant, the densities of R. similis determined in flowering plants tended in most cases to overestimate the populations in the succeeding suckers. Sampling flowering plants at Pococí (70 masl overestimated Helicotylenchus spp. populations in the succession suckers of both cultivars, while those at Matina (40 masl were underestimated. The estimation of Meloidogyne spp. and Pratylenchus spp. populations was also not reliable. To decide on nematicide applications, it is recommended to sample the succeeding suckers of recently flowering plants.

  16. Isolation of MA-ACS Gene Family and Expression Study of MA-ACS1 Gene in Musa acuminata Cultivar Pisang Ambon Lumut

    Directory of Open Access Journals (Sweden)

    LISTYA UTAMI KARMAWAN

    2009-03-01

    Full Text Available Musa acuminata cultivar pisang ambon lumut is a native climacteric fruit from Indonesia. Climacteric fruit ripening process is triggered by the gaseous plant hormone ethylene. The rate limiting enzyme involved in ethylene biosynthesis is ACC synthase (ACS which is encoded by ACS gene family. The objective of this study is to identify MA-ACS gene family in M. acuminata cultivar pisang ambon lumut and to study the MA-ACS1 gene expression. The result showed that there were nine M. acuminata ACS gene family members called MA-ACS1–9. Two of them (MA-ACS1 and MA-ACS2 were assessed using reverse transcriptase PCR (RT-PCR for gene expression study and it was only MA-ACS1 correlated with fruit ripening. The MA-ACS1 gene fragment has been successfully isolated and characterized and it has three introns, four exons, and one stop codon. It also shows highest homology with MACS1 gene from M. acuminata cultivar Hsian Jien Chiao (GenBank accession number AF056164. Expression analysis of MA-ACS1 using quantitative PCR (qPCR showed that MA-ACS1 gene expression increased significantly in the third day, reached maximum at the fifth day, and then decreased in the seventh day after harvesting. The qPCR expression analysis result correlated with the result of physical analysis during fruit ripening.

  17. Expansion of banana (Musa acuminata) gene families involved in ethylene biosynthesis and signalling after lineage-specific whole-genome duplications.

    Science.gov (United States)

    Jourda, Cyril; Cardi, Céline; Mbéguié-A-Mbéguié, Didier; Bocs, Stéphanie; Garsmeur, Olivier; D'Hont, Angélique; Yahiaoui, Nabila

    2014-05-01

    Whole-genome duplications (WGDs) are widespread in plants, and three lineage-specific WGDs occurred in the banana (Musa acuminata) genome. Here, we analysed the impact of WGDs on the evolution of banana gene families involved in ethylene biosynthesis and signalling, a key pathway for banana fruit ripening. Banana ethylene pathway genes were identified using comparative genomics approaches and their duplication modes and expression profiles were analysed. Seven out of 10 banana ethylene gene families evolved through WGD and four of them (1-aminocyclopropane-1-carboxylate synthase (ACS), ethylene-insensitive 3-like (EIL), ethylene-insensitive 3-binding F-box (EBF) and ethylene response factor (ERF)) were preferentially retained. Banana orthologues of AtEIN3 and AtEIL1, two major genes for ethylene signalling in Arabidopsis, were particularly expanded. This expansion was paralleled by that of EBF genes which are responsible for control of EIL protein levels. Gene expression profiles in banana fruits suggested functional redundancy for several MaEBF and MaEIL genes derived from WGD and subfunctionalization for some of them. We propose that EIL and EBF genes were co-retained after WGD in banana to maintain balanced control of EIL protein levels and thus avoid detrimental effects of constitutive ethylene signalling. In the course of evolution, subfunctionalization was favoured to promote finer control of ethylene signalling. PMID:24716518

  18. Intellectual property protection through the geographical indication “Chã de Jardim” of the banana leaf handicrafts (musa sp. in the State of Paraiba

    Directory of Open Access Journals (Sweden)

    Everaldo de França

    2015-11-01

    Full Text Available The construction of a Brazilian Geographical Indication System has been guided by a far wider range of goods, ranging from industrial and agricultural products, services, and even craftwork. Taking into account social, cultural and geographical characteristics, as well as the ethnic constitution of the population of Areia city in the State of Paraiba (Brazil, it is possible to suppose the vast local knowledge that this city’s communities have accumulated over the decades while they seek to incorporate such knowledge in their daily practices. One of the artesanal activities subject to protection of Geographical Indication (IG is the use of banana’s plant (Musa sp. stem as a raw material for some  manufactured products such as handbags, light fixtures, folders for events, cooking utensils etc. The IG records for this type of activity can value the local knowledge regarding the sustainable use of biodiversity and promote the cultural traditions of Areia city, bringing also improvements to the communities involved and consequently increasing the regional development. The identified geographical name to be protected for craftwork is “Chã de Jardim”. It is suggested that “Chã de Jardim” becomes protected by indication of origin whereas the artesanal products of this region feature good reputation. JEL-Code | O33; Q56; R11.

  19. Identification of Biomarkers for Resistance to Fusarium oxysporum f. sp. cubense Infection and in Silico Studies in Musa paradisiaca Cultivar Puttabale through Proteomic Approach

    Directory of Open Access Journals (Sweden)

    Venkatesh Ramu

    2016-02-01

    Full Text Available Panama wilt caused by Fusarium oxysporum f. sp. cubense (Foc is one of the major disease constraints of banana production. Previously, we reported the disease resistance Musa paradisiaca cv. puttabale clones developed from Ethylmethanesulfonate and Foc culture filtrate against Foc inoculation. Here, the same resistant clones and susceptible clones were used for the study of protein accumulation against Foc inoculation by two-dimensional gel electrophoresis (2-DE, their expression pattern and an in silico approach. The present investigation revealed mass-spectrometry identified 16 proteins that were over accumulated and 5 proteins that were under accumulated as compared to the control. The polyphosphoinositide binding protein ssh2p (PBPssh2p and Indoleacetic acid-induced-like (IAA protein showed significant up-regulation and down-regulation. The docking of the pathogenesis-related protein (PR with the fungal protein endopolygalacturonase (PG exemplify the three ionic interactions and seven hydrophobic residues that tends to good interaction at the active site of PG with free energy of assembly dissociation (1.5 kcal/mol. The protein-ligand docking of the Peptide methionine sulfoxide reductase chloroplastic-like protein (PMSRc with the ligand β-1,3 glucan showed minimum binding energy (−6.48 kcal/mol and docking energy (−8.2 kcal/mol with an interaction of nine amino-acid residues. These explorations accelerate the research in designing the host pathogen interaction studies for the better management of diseases.

  20. Antidiabetic and Antilipidemic Effect of Musa balbisiana Root Extract: A Potent Agent for Glucose Homeostasis in Streptozotocin-Induced Diabetic Rat

    Science.gov (United States)

    Kalita, Himadri; Boruah, Dulal C.; Deori, Meetali; Hazarika, Ankita; Sarma, Rahul; Kumari, Sima; Kandimalla, Raghuram; Kotoky, Jibon; Devi, Rajlakshmi

    2016-01-01

    Folklore studies have revealed that Musa balbisiana Colla (MB; Family: Musaceae) has high medicinal properties. The purpose of the present study is to evaluate antihyperglycemic, and antioxidant activity of MB extracts in streptozotocin (STZ) induced diabetic rats. In vitro antioxidant and antidiabetic activity of MB extracts, i.e., root extract (RE), shoot extract and inflorescence extract were determined by using various methods viz 1,-1-diphenyl-2-picrylhydrazyl (DPPH) and a method to assess their possible effect on glucose diffusion across gastrointestinal tract and identify bioactive compound of potent extract. In vivo antilipidemic and antidiabetic activity was evaluated by administrating oral dose of RE for 15 days on STZ- induced diabetic rat. RE showed highest antioxidant activity by scavenging DPPH radical (IC50 32.96 μg/ml) and inhibit 30% glucose movement in vitro. The methanol extract of root showed the presence of calyx [4] arene category of the compound. Furthermore, RE treated rat revealed a reduction in fasting blood glucose (62.5%), serum total cholesterol (36.2%), triglyceride (54.5%), and low-density lipoprotein (50.94%) after 15 days as compared to STZ treated animal. There was an initiation of regenerative structures of the affected organs after 15 days of RE treatment. Histopathological observations clearly differentiate the structural changes in pancreas, liver, and kidney of STZ and RE treated group. The presence of calyx [4] arene class of compound may be responsible for its antioxidant and antidiabetic properties by absorbing glucose in vivo. PMID:27199747

  1. Engagement of Arginine Finger to ATP Triggers Large Conformational Changes in NtrC1 AAA+ ATPase for Remodeling Bacterial RNA Polymerase

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Baoyu; Sysoeva, Tatyana A.; Chowdhury, Saikat; Guo, Liang; De Carlo, Sacha; Hanson, Jeffrey A.; Yang, Haw; Nixon, B. Tracy (IIT); (UCB); (City U/NY); (Penn)

    2010-11-19

    The NtrC-like AAA+ ATPases control virulence and other important bacterial activities through delivering mechanical work to {sigma}54-RNA polymerase to activate transcription from {sigma}54-dependent genes. We report the first crystal structure for such an ATPase, NtrC1 of Aquifex aeolicus, in which the catalytic arginine engages the {gamma}-phosphate of ATP. Comparing the new structure with those previously known for apo and ADP-bound states supports a rigid-body displacement model that is consistent with large-scale conformational changes observed by low-resolution methods. First, the arginine finger induces rigid-body roll, extending surface loops above the plane of the ATPase ring to bind {sigma}54. Second, ATP hydrolysis permits Pi release and retraction of the arginine with a reversed roll, remodeling {sigma}54-RNAP. This model provides a fresh perspective on how ATPase subunits interact within the ring-ensemble to promote transcription, directing attention to structural changes on the arginine-finger side of an ATP-bound interface.

  2. 資産負債中心主義と収益費用中心主義の比較 -FASB概念ステートメント,会社会計基準序説,AAA1957年会計原則-

    OpenAIRE

    岡田, 裕正

    1995-01-01

    This paper compares the accounting structures in the FASB Conceptual Statements, the Paton-Littleton's "An Introduction to Corporate Accounting Standards" and the AAA's Accounting Standard in 1957. As many researchers say, FASB's Conceptual Statements has Asset-and-Liability View and Paton-Littleton's monograph has Revenue-and-Expense View. These two have different accounting structure. And this paper also clarifies that the accounting structure of the AAA's standard is placed between Asset-a...

  3. Efectos de recubrimientos de almidón de yuca, ácido ascórbico, n-acetil-cisteína en la calidad del plátano (musa paradisiaca)

    OpenAIRE

    Palacín Beltrán, José Roberto

    2012-01-01

    El efecto de recubrimientos funcionales sobre la calidad de frutos de plátano Hartón (Musa paradisiaca) fue evaluado en la etapa de poscosecha. Las soluciones fueron preparadas a partir de almidón de yuca comercial (Maniot esculenta) 50 g/L, como plastificantes se usaron glicerol 30 g/L y polietilen glicol@600® 6 g/L, se adicionaron como antipardeantes, ácido ascórbico (AC) 6 g/L, (correspondiente al tratamiento 1 @ tr 1), N@acetil@cisteína (NAC) 8 g/L (concer...

  4. PBC算法与AAA算法在肺癌调强放疗中的剂量学比较%Dosimetric Comparison of PBC and AAA Dose Calculation Algorithm for IMRT Treatment of Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    张富利; 王军良; 盛洪国; 郑明民

    2011-01-01

    Objective: To compare the dosimetric differences between intensity modulated radiotherapy (IMRT) plans for non-small cell lung cancer using pencil beam convolution (PBC) and anisotropic analytical algorithm (AAA) methods.Materials and Methods: For a cohort of 7 patients, IMRT plans were designed using PBC and AAA calculation methods, respectively.In the planning process, identical planning objectives and dose constraints were set.Plans were compared according to dose-volume histogram (DVH) analysis in terms of PTV homogeneity and conformity indices (HI and CI) as well as OARs dose and volume parameters.Results: On average, no statistically significant differences between PBC and AAA plans were observed except Dmean and VI0 of lung.Conclusions: Both PBC and AAA algorithms provide similar dose computation accuracy for NSCLC,but the latter is less affected by the variation of respiratory phase and suggested as a priority choice.%目的:分析、比较笔形束卷积算法(PBC)和各向异性解析算法(AAA)在非小细胞肺癌(NSCLC)调强放疗计划设计中的剂量学差异.方法:随机选择7例NSCLC患者,采用Eclipse version 7.3.10计划系统提供的PBC算法和AAA算法对每例NSCLC进行IMRT的计划设计,比较靶区及危及器官的剂量分布、DVH等指标.结果:两种算法获得治疗计划的靶区剂量均匀性和适形度均无明显差别,食管、心脏、脊髓等危及器官的受量也基本相同.结论:对于NSCLC,剂量计算应采用受呼吸时相影响更小的AAA算法.

  5. Variação somaclonal em mudas micropropagadas de bananeira, cultivar Pacovan Somaclonal variation event on micropropagated Pacovan banana seedling (Musa spp. AAB group

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    Cynthia Christina Carvalho dos Santos

    2004-01-01

    Full Text Available A literatura tem relatado a ocorrência de variação somaclonal em plantas derivadas de cultura de tecidos. Neste trabalho, foi estudada a influência do número de subcultivos na indução de variantes em bananeira, cultivar Pacovan (Musa spp., grupo AAB. Ápices caulinares foram introduzidos e multiplicados in vitro, utilizando-se no estabelecimento o meio de cultura MS com adição de 2,5 mg.L-1 de BAP e nos subcultivos subseqüentes, o meio MS com 4,0 mg.L-1 de BAP para indução de brotações laterais. Foram obtidas gerações com diferentes números de subcultivos (3, 4, 5, 6, 7, 8 e 9, e comparadas com mudas obtidas de rizomas. Observaram-se as plantas obtidas, no total de 426, em condições de campo no Vale do Açu (RN. Para cálculo da porcentagem de variantes somaclonais, avaliaram-se as seguintes características morfológicas: estatura da planta, coloração e forma das folhas e conformação do cacho. Foram consideradas como variantes apenas as plantas cujos perfilhos também mostravam o mesmo tipo de variação. Constatou-se a ocorrência de variação somaclonal nas mudas provenientes dos tratamentos a partir de cinco subcultivos, atingindo-se o valor de 5,8% de variantes no nono subcultivo. O aumento da porcentagem de variação somaclonal com o do número de subcultivos indica o cuidado que as biofábricas devem ter em relação a esse parâmetro, desenvolvendo protocolos de micropropagação específicos para cada cultivar a ser comercializada.The occurrence of somaclonal variation for plants in developed from tissue culture is well documented in the literature. The present study evaluated the influence of numbers subculture in the induction of variants in Pacovan banana tree (Musa spp., AAB group. Apex stems were introduced and multiplied in vitro using culture media MS, with addition of 2.5 mg.L-1 of BAP. In subsequent subcultures, MS with 4.0 mg.L-1 of BAP was used to induce side buds. Results showed that plants were

  6. A Ser/Thr protein kinase phosphorylates MA-ACS1 (Musa acuminata 1-aminocyclopropane-1-carboxylic acid synthase 1) during banana fruit ripening.

    Science.gov (United States)

    Choudhury, Swarup Roy; Roy, Sujit; Sengupta, Dibyendu N

    2012-08-01

    1-Aminocyclopropane-1-carboxylic acid synthase (ACS) catalyzes the rate-limiting step in ethylene biosynthesis during ripening. ACS isozymes are regulated both transcriptionally and post-translationally. However, in banana, an important climacteric fruit, little is known about post-translational regulation of ACS. Here, we report the post-translational modification of MA-ACS1 (Musa acuminata ACS1), a ripening inducible isozyme in the ACS family, which plays a key role in ethylene biosynthesis during banana fruit ripening. Immunoprecipitation analyses of phospholabeled protein extracts from banana fruit using affinity-purified anti-MA-ACS1 antibody have revealed phosphorylation of MA-ACS1, particularly in ripe fruit tissue. We have identified the induction of a 41-kDa protein kinase activity in pulp at the onset of ripening. The 41-kDa protein kinase has been identified as a putative protein kinase by MALDI-TOF/MS analysis. Biochemical analyses using partially purified protein kinase fraction from banana fruit have identified the protein kinase as a Ser/Thr family of protein kinase and its possible involvement in MA-ACS1 phosphorylation during ripening. In vitro phosphorylation analyses using synthetic peptides and site-directed mutagenized recombinant MA-ACS1 have revealed that serine 476 and 479 residues at the C-terminal region of MA-ACS1 are phosphorylated. Overall, this study provides important novel evidence for in vivo phosphorylation of MA-ACS1 at the molecular level as a possible mechanism of post-translational regulation of this key regulatory protein in ethylene signaling pathway in banana fruit during ripening. PMID:22419220

  7. Caracterización de harina y almidón obtenidos a partir de plátano guineo AAAea (Musa sapientum L.

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    Juan Carlos Lucas

    2013-04-01

    Full Text Available En el estudio se caracterizaron las propiedades química, térmica, funcional y morfológica de la harina y el almidón de guineo AAAea (Musa sapientum L.. Los resultados mostraron un rendimiento de 33.33% para harina y 3.61% para almidón. El porcentaje de proteína en la harina fue de 5.43% y en almidón de 2.17%. La fibra soluble en harina fue de 19.85% y de grasa en el almidón de 4.11 %. El análisis térmico de la harina mostró que la etapa de descomposición varía desde 141.33 °C hasta 388.30 °C con una pérdida de peso de 55.85%. Para el almidón esta misma condición ocurrió entre los 248 °C y 327 °C con una pérdida de peso de 74.15%. Las temperaturas de gelatinización para harina y almidón fueron de 68 y 66.41 °C, respectivamente, con entalpías de gelatinización 2.38 J/g para harina y 6.06 J/g para almidón. En el análisis de RVA durante el período de enfriamiento, tanto la harina como el almidón presentaron buena viscosidad. La mayor parte de los gránulos de almidón mostraron formas irregulares, aunque algunos tienen forma esférica u oval. Los tamaños varían desde 4.17 µm hasta 42.11 µm.

  8. Efectos de dos sistemas de producción en el contenido de minerales en el fruto de Musa AAB Simmonds

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    Guerra Hernández Katty Julia

    2011-12-01

    Full Text Available Los sistemas de producción orgánico y/o ecológico tienen como objetivo garantizar la sostenibilidad y la renovación de base natural, mediante la limitación del uso de productos de síntesis química para favorecer el ambiente y la salud humana. Teniendo en cuenta estas características, se estudió el efecto de los sistemas de producción orgánico y convencional sobre el contenido de nutrientes minerales del fruto del plátano Hartón (Musa AAB Simmonds, durante el proceso de maduración. Las determinaciones se realizaron en la Universidad de Córdoba (Montería, Colombia con el fin de establecer las diferencias en el contenido de minerales en los frutos en ambos sistemas y en diferentes estados de maduración. Se utilizó un diseño completamente al azar con arreglo factorial 2 x 5 y cinco repeticiones. Los factores fueron: sistema de producción y estado de maduración; los cinco niveles del segundo factor correspondieron a los grados de maduración: verde-oscuro (V, verde-claro (VC, amarillo-verde (AV, amarillo (A y muy amarillo (MA. Los resultados obtenidos muestran que entre ambos sistemas de producción y en distintos grados de maduración, se presentan diferencias significativas (P < 0.05 en los contenidos de calcio, magnesio, potasio, sodio, hierro y zinc.

  9. Efectos de dos sistemas de producción en el contenido de minerales en el fruto de Musa AAB Simmonds

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    José Luis Barrera-Violeth

    2011-12-01

    Full Text Available Los sistemas de producción orgánico y/o ecológico tienen como objetivo garantizar la sostenibilidad y la renovación de base natural, mediante la limitación del uso de productos de síntesis química para favorecer el ambiente y la salud humana. Teniendo en cuenta estas características, se estudió el efecto de los sistemas de producción orgánico y convencional sobre el contenido de nutrientes minerales del fruto del plátano Hartón (Musa AAB Simmonds, durante el proceso de maduración. Las determinaciones se realizaron en la Universidad de Córdoba (Montería, Colombia con el fin de establecer las diferencias en el contenido de minerales en los frutos en ambos sistemas y en diferentes estados de maduración. Se utilizó un diseño completamente al azar con arreglo factorial 2 x 5 y cinco repeticiones. Los factores fueron: sistema de producción y estado de maduración; los cinco niveles del segundo factor correspondieron a los grados de maduración: verde-oscuro (V, verde-claro (VC, amarillo-verde (AV, amarillo (A y muy amarillo (MA. Los resultados obtenidos muestran que entre ambos sistemas de producción y en distintos grados de maduración, se presentan diferencias significativas (P < 0.05 en los contenidos de calcio, magnesio, potasio, sodio, hierro y zinc.

  10. Evaluation of gamma irradiated abaca (Musa textiles Nee.) for resistance to abaca bunchy top virus and banana bract mosaic virus under screen house condition

    International Nuclear Information System (INIS)

    Abaca (Musa textilis Nee.) is the source of natural strong fiber in the Philippines. There has been decreasing production of abaca fibers in the last decade since the available commercial varieties are susceptible to the two major viral diseases, namely bunchy top and bract mosaic. In vitro technology coupled with gamma irradiation (60Cobalt) were sought in order to develop varieties with resistance to these two viruses. To start with the irradiation of two varieties, namely Tinawagan Pula and Tangongon, the optimum dose level or lethal dose or LD50 of 60Cobalt was established by taking the rate of shoot proliferation and growth development of shoot cultures (Sub Cycle 1 to 3). After bulk irradiation using the developed LD50, all plantlets were inoculated with abaca bunchy top virus and banana bract mosaic virus using insect transmission and mechanical transmission, respectively. Out of the 2,296 plants of variety Tinawagan Pula and 974 plants of variety Tangongon, 43 plants or 1.9% and 9 plants or 0.9%, respectively, were negative to abaca bunchy top virus using Enzyme-linked immunoassay (ELISA). For bract mosaic, from the 2,169 plants of variety Tinawagan Pula, and 1,006 plants of variety Tangongon, 57 plant or 2.6% of variety Tinawagan Pula and 14 plants or 1.4% of variety Tangongon, were negative to banana bract mosaic virus using Polymerase Chain Reaction (PCR). The putatively resistant lines of these two varieties from the screen house experiment are being micro-propagated for field evaluation. (author)

  11. Tiempo de almacenamiento del cormo y su efecto en el crecimiento y producción de plátano (Musa AAB Dominico Hartón

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    Manuel Aristizábal L

    2013-12-01

    Full Text Available Se evaluó el efecto del tiempo de almacenamiento del material de siembra sobre el crecimiento y producción del plátano (Musa AAB, utilizando cormos de plantas sanas libres de tejido necrosado.La mitad de ellos fueron sumergidos durante 60 min en una solución de 15 g de carbofurán y 20 g de manzate en 10 L de agua, después de lo cual tanto los cormos tratados como los no tratados fueron colocados sobre el suelo y cubiertos con hojas frescas de plátano. Cada semana, durante un período de 6 semanas, se tomó la cantidad necesaria de cormos para plantar a una distancia de 3m entre surcos y 2m entre plantas, en un diseño de parcelas divididas con cuatro repeticiones y 10 plantas por repetición. En floración se hicieron registros de altura de planta, diámetro del pseudotallo, número de hojas funcionales presentes y hojas jóvenes manchadas; en cosecha se registró el peso de los racimos y se calculó el peso promedio del dedo en cada uno de ellos. Se encontró que el almacenamiento del cormo reduce el crecimiento de la planta sin afectar la severidad de la Sigatoka del plátano, igualmente reduce de forma severa y significativa el peso del racimo y el tamaño de dedo; no obstante, el tratamiento preventivo del cormo tiene efecto positivo en el crecimiento y producción de la planta, aunque no reduce el daño causado por el envejecimiento del material de propagación.

  12. Effect of cassava-starch coatings with ascorbic acidic and N-acetylcysteine on the quality of harton plantain (Musa paradisiaca

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    Carlos Julio Márquez Cardozo

    2015-06-01

    Full Text Available The effect of coatings was evaluated on the quality ofharton plantain fruits (Musa paradisiaca in the postharvest stage.Solutions for three treatments were prepared from 50 g L-1 cassavastarch (Manihot esculenta with 30 g L-1 glycerol as the plasticizerand 6 g L-1 polyethylene glycol-600®; for anti-browning agents, 6g L-1 ascorbic acid (AA and 8 g L-1 N-Acetyl-Cysteine (NAC wereadded. The fruits were coated by immersion, stored at 18 ± 4°Cand 85% RH. Physicochemical properties were determined at 32days postharvest. The applied coatings decreased the physiologicalweight loss (%WL and resulted in a higher pulp firmness (PF; nosignificant difference was seen with a confidence level of 95% inthe concentration of total soluble solids (TSS, acidity or maturityindex. The skin color was measured by the CIE L*a*b* method, withan average L value of 70 for the fruits coated with the 6 g L-1 AAand 8 g L-1 NAC mixture, while the control fruits had a value of 57.Also, lower a* coordinate values and browning indices were foundfor the epidermis of the coated fruits. The enzymatic activity of thepolyphenol oxidase decreased with the number of postharvest daysfor all of the treatments, being lower for the fruits with the mixtureof anti-browning agents by 27%, as compared to the control. It wasconcluded that the coating mixture containing the anti-browningagents ascorbic acid, 6 g L-1, and N-acetyl-cysteine, 8 g L-1, showeda better effect as an alternative for storing fruits and prolongingthe shelf-life of harton plantain.

  13. CRESCIMENTO E OXIDAÇÃO DE EXPLANTES DE BANANEIRA-PRATA (Musa AAB IN VITRO: I. CONCENTRAÇÕES DE SAIS DE FERRO, COBRE E ZINCO

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    UTINO SERGIO

    2001-01-01

    Full Text Available Este experimento teve como objetivo avaliar a influência de diferentes concentrações de ferro, cobre e zinco do meio MS (MURASHIGE & SKOOG, 1962 no controle da oxidação de explantes iniciais de bananeira-Prata (Musa AAB. Foram utilizadas três concentrações (100, 50 e 0 miM de FeEDTA, duas concentrações (0,1 e 0miM de (CuSO4.5H2O e duas concentrações (30 e 0miM de (ZnSO4.7H2O, num delineamento inteiramente casualizado, arranjado em um fatorial completo 3 x 2 x 2, utilizando-se de 15 repetições. Ápices caulinares foram inoculados em meio MS modificado e, decorridos 28 dias após a inoculação, avaliaram-se a massa de matéria fresca, altura e grau de oxidação. Observou-se que esses micronutrientes são essenciais para o crescimento dos explantes e que a concentração de ferro influencia na oxidação de explantes, sendo que maiores graus de escurecimento foram observados nas concentrações mais elevadas. A redução ou retirada destes elementos do meio MS, isoladamente ou em combinações, não foi suficiente para eliminar a oxidação dos explantes.

  14. Suitability of Pueraria phaseoloides, Chromolaena odorata and Tithonia diversifolia as in-situ mulch for nematode management in musa cropping systems.

    Science.gov (United States)

    Schösser, B; Hauser, S; Sikora, R A

    2006-01-01

    Mulching with plant organic matter has been shown to reduce nematode population densities in various cropping systems. The level of nematode control is increased when such mulches are incorporated into the soil as organic amendments. Chromolaena odorata, Tithonia diversifolia and Pueraria phaseoloides are common cover crops in West and Central Africa that produce large quantities of nutrient rich biomass. The aim of this study was to determine, if in-situ mulching of C. odorata, T. diversifolia and P. phaseoloides is suitable for nematode control in Musa production. In a pot trial, the susceptibility of these plants to spiral nematodes was investigated. The effects of different quantities of surface mulch on nematode population densities in the soil and in banana roots also were determined. All mulch types and all quantities led to a reduction in nematode population densities in the soil. The strongest nematode reductions were observed in the Pueraria treatments. In treatments containing banana plants mulching improved plant growth compared to the clean-fallowed soil and induced lower root infestation rates. However, nematode soil populations were higher in mulched than in non-mulched banana treatments. Plant parasitic nematodes also were isolated from roots of all three cover crop species and all three plants caused an increase in nematode numbers in the soil. Therefore, the tested cover crops proved unsuitable for nematode control in a system with the highly susceptible bananas. Further examinations are needed to determine whether or not the positive effects of surface mulching on plantain plant growth and root infestation rates also have positive effects on yield in an in-situ mulching system in the presence of nematodes. PMID:17390809

  15. Les compositions mémorielles autour de la Guerre patriotique. L’exemple du souvenir de Moussa Djalil, Tatar, stalinien, poète et patriote Memory Schemes Around the Patriotic War. The Example of the Memory of Musa Dzhalil, Tatar, Stalinist, Poet and Patriot

    Directory of Open Access Journals (Sweden)

    Françoise Daucé

    2012-01-01

    Full Text Available This article proposes to study the memorial destiny of Musa Dzhalil, a Tatar poet well-known in Russia for his Moabit Notebook written in the Berlin prison where he died in 1944. After 1956, he became posthumously an official Hero of the Soviet Union and won the Lenin Prize for his work. His popularity survived the disappearance of the USSR and Musa Dzhalil is still a popular figure in Russia today. This popularity is the result of a complex combination of factors : he is celebrated as a great patriot (for his military exploit in Germany but also as a representative of the Tatar culture (he wrote his work in Tatar, as an artist (his poems were translated into Russian and, last but not least, as a representative of the Soviet society (of the Stalinist period. Because of the mixing of these different components, the cult of Musa Dzhalil can be appropriated simultaneously by different groups in Post-Soviet society (Russian leadership, Tatar authorities, Communist Party, Cultural institutions. This complex combination of supports forbids any critical assessment of Dzhalil’s memory. Critics against him are immediately denounced as anti-Tatar, anti-Soviet, anti-cultural and anti-Russian. This paper thus shows, through the example of Musa Dzhalil, the insertion of Great Patriotic War memory in a complex collection of social and political bonds.

  16. EFEITO DA EMBALAGEM COM SACOS DE POLIETILENO NO COMPORTAMENTO DO AMIDO NA POLPA DE BANANA DO CULTIVAR PRATA (Musa sp. NO PERÍODO DE CONSERVAÇÃO E MATURAÇÃO EFFECT OF POLYETHYLENE PACKING BAGS ON THE STARCH BEHAVIOR IN THE CONSERVATION MATURATION PERIOD OF BANANA PULP CULTIVAR “PRATA” (Musa sp.

    Directory of Open Access Journals (Sweden)

    Lincoln F. Zica

    2007-09-01

    Full Text Available

    Neste trabalho estudou-se o comportamento do amido da polpa de banana do cultivar “Prata" (Musa sp., sob os seguintes tratamentos: Testemunha, sem embalagem; Plástico perfurado; Plástico perfurado, contendo absorvente; Plástico sem perfuração; Plástico, sem perfuração, contendo absorvente. As bananas tratadas, foram acondicionadas em uma câmara com temperatura constante de 22 ± 1°C e umidade relativa de 90 ± 3%. Uma vez por dia era feita a ventilação da câmara. De dois em dois dias, retiravam-se amostras de cada tratamento e faziam-se as análises. Com auxílio das análises estatísticas dos dados obtidos conclui-se que: a. A embalagem com polietileno atrasa o início da queda do amido, mas não impede a sua transformação. b. a percentagem de amido na polpa, permanece constante até que, em dado momento, que varia com os tratamentos, inicia a sua variação. c. O uso de absorvente de etileno dentro da embalagem plástica, sem perfuração, atrasa mais o início da queda do amido, mas não impede que os frutos se tornem imprestáveis para o consumo.

    In this experiment, the behavior of starch in the pulp of banana Prata (Musa sp., was studied under the following treatments: bananas without packing, perforated plastic, perforated plastic with an absorbent, unperforated plastic, unperforated plastic containing an absorbent. The treated bananas, were shelved at 22 ± 1°C and a relative humidity of 90 ± 3%. Every 2 days, samples of each treatment were collected and analysis were made. With the help of statistical analyses of the data, the following conclusions were reached: a. The packaging with polyethylene delays the starch breakdown, but does not impede its transformation. b. The percentage of starch of the pulp remains constant until a given moment, which varies with each treatment, when its

  17. ETHIOLOGICAL ASPECTS OF BANANA BUNCH DRYING OCCURRENCE IN Musa sp. AAB Cv. PRATA IN THE GOIÁS STATE, BRAZIL ASPECTOS ETIOLÓGICOS DA OCORRÊNCIA DA SECA DO CACHO DA BANANEIRA PRATA (Musa sp. AAB NO ESTADO DE GOIÁS

    Directory of Open Access Journals (Sweden)

    Wilma Alves Ferrari

    2007-09-01

    Full Text Available

    The purpose of the present study was to determine the cause of the drying of the bunch in the “Prata banana” (Musa sp. AAB and to analyze some of the aspects of its occurrence in the State of Goiás. The disease drastically affects production, and occurs more severely during the rainy season (October to April , going so far as to affect almost all of the bunches in certain regions. Based on the results obtained in isolation, under aseptic conditions, and using Koch’s postulate, the primary cause of the disease was determined to be the fungus Gloesporium musarum Cooke & Massee, which acts under predisposing climatic conditions. It was suggested that clonal characteristics, climatic conditions, and the potential of available innoculum were the principal factors involved in the spread of the disease in certain regions.

    No presente trabalho procurou-se determinar a causa da “seca do cacho” de banana do cultivar Prata (Musa sp. AAB e analisar alguns aspectos de sua ocorrência no Estado de Goiás. A doença afeta drasticamente a produção, e ocorre mais severamente durante o período chuvoso (outubro - abril, podendo, em certas regiões, afetar quase todos os cachos. Mediante os resultados obtidos em isolamentos sob condições axênicas e através da aplicação do postulado de Koch, determinou-se que a causa primária da doença á o fungo Gloeosporium musarum Cooke & Massee, atuando sob condições climáticas predisponentes. Foi sugerido que características clonais, condições climáticas e potencial de inóculo disponível sejam os principais fatores envolvidos na distribuição regional da enfermidade.

  18. Granules morphology and rheological behavior of green banana (Musa cavendishii and corn (Zea mays starch gels Morfologia dos grânulos e comportamento reológico dos géis de amido de banana verde (Musa cavendishii e milho (Zea mays

    Directory of Open Access Journals (Sweden)

    Dayane Rosalyn Izidoro

    2007-10-01

    Full Text Available In this work, it was used starch obtained from green banana (Musa cavendishii and commercial corn (Zea mays starch in order to compare the granule morphology and the rheological behavior of these gel-starches. Images of starch granules morphology were obtained from scanning electron microscope (SEM. The banana starch granules presented an oval and ellipsoidal shape with irregular diameters. Neverthless, the granules of corn starch showed a poliedric shape, with different sizes. The rheological behavior of gel starch solutions showed a non-newtonian character with a pseudoplastic behavior. Herschel-Bulkley model gave a good description on the rheological behavior of the gel starch. Banana gel-starch solutions showed higher values of shear stress and apparent viscosity when compared with corn gel-starch solutions. A progressive decrease in shear stress and viscosity occurred with the addition of sodium chloride and sucrose.No presente trabalho foi utilizado amido obtido de banana verde (Musa cavendishii e amido de milho (Zea mays comercial, com o objetivo de comparar a morfologia dos grânulos e o comportamento reológico dos géis. As imagens da morfologia dos grânulos foram obtidas por microscopia eletrônica de varredura. Os grânulos de amido da banana apresentaram forma oval e elipsoidal com diâmetros irregulares, todavia, os grânulos do amido de milho mostraram forma poliédrica e diferentes tamanhos. As análises reológicas dos géis das soluções de amido mostraram caráter não-newtoniano, pseudoplástico. O modelo de Herschel-Bulkley foi o que melhor representou comportamento reológico dos géis. O gel de amido de banana verde obteve maiores valores de tensão de cisalhamento e viscosidade aparente quando comparada ao gel de amido de milho. Ocorreu um decréscimo progressivo na tensão de cisalhamento e na viscosidade com a adição de cloreto de sódio e sacarose aos géis.

  19. NVL2, a nucleolar AAA-ATPase, is associated with the nuclear exosome and is involved in pre-rRNA processing

    Energy Technology Data Exchange (ETDEWEB)

    Yoshikatsu, Yuki [Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, Tokushima 770-8506 (Japan); Ishida, Yo-ichi; Sudo, Haruka [Department of Molecular and Cellular Biochemistry, Meiji Pharmaceutical University, Kiyose, Tokyo 204-8588 (Japan); Yuasa, Keizo; Tsuji, Akihiko [Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, Tokushima 770-8506 (Japan); Nagahama, Masami, E-mail: nagahama@my-pharm.ac.jp [Department of Molecular and Cellular Biochemistry, Meiji Pharmaceutical University, Kiyose, Tokyo 204-8588 (Japan)

    2015-08-28

    Nuclear VCP-like 2 (NVL2) is a member of the chaperone-like AAA-ATPase family and is involved in the biosynthesis of 60S ribosomal subunits in mammalian cells. We previously showed the interaction of NVL2 with a DExD/H-box RNA helicase MTR4/DOB1, which is a known cofactor for an exoribonuclease complex, the exosome. This finding implicated NVL2 in RNA metabolic processes during ribosome biogenesis. In the present study, we found that a series of mutations within the ATPase domain of NVL2 causes a defect in pre-rRNA processing into mature 28S and 5.8S rRNAs. Co-immunoprecipitation analysis showed that NVL2 was associated with the nuclear exosome complex, which includes RRP6 as a nucleus-specific catalytic subunit. This interaction was prevented by depleting either MTR4 or RRP6, indicating their essential role in mediating this interaction with NVL2. Additionally, knockdown of MPP6, another cofactor for the nuclear exosome, also prevented the interaction by causing MTR4 to dissociate from the nuclear exosome. These results suggest that NVL2 is involved in pre-rRNA processing by associating with the nuclear exosome complex and that MPP6 is required for maintaining the integrity of this rRNA processing complex. - Highlights: • ATPase-deficient mutants of NVL2 have decreased pre-rRNA processing. • NVL2 associates with the nuclear exosome through interactions with MTR4 and RRP6. • MPP6 stabilizes MTR4-RRP6 interaction and allows NVL2 to interact with the complex.

  20. Preliminary evaluation of solvent-extraction and/or ion-exchange process for meeting AAA program multi-tier systems recovery and purification goals

    International Nuclear Information System (INIS)

    Several potential processes are described and evaluated for their suitability in a multitier aqueous-based approach to processing dissolved spent nuclear fuel under the Advanced Accelerator Applications (AAA) program. The evaluation is focused on solvent extraction and ion exchange technologies that have been demonstrated to varying degrees. The goals of the program are to separate uranium (U), technetium (Tc), and the transuranic (TRU) elements from the fission products that are to be vitrified for disposal as high-level waste (HLW). Uranium will be disposed as low-level waste (LLW); Tc and TRU will be transmuted in an accelerator. A number of processes have been examined. The focus was on liquid-liquid solvent extraction processes because of their relatively high state of development and their suitability for high-throughput-rate processing. Ion exchange processes were also examined. PUREX and UREX were evaluated as options for recovery of uranium; UREX is also an option for Tc recovery. Solvent extraction options examined for TRU recovery included TRUEX, DIAMEX, and TRPO, as well as some based on TBP extraction. Processes for trivalent actinide separation from lanthanides were also examined. The PUREX processes have been developed over many years, and have been refined to a significant degree. In the first cycle, U and plutonium (Pu) are co-extracted from dissolved spent fuel solutions by TBP in a diluent, selectively stripped, and purified in additional extraction/strip cycles. Neptunium (Np) can be extracted or driven into the raffinate by adjusting the oxidation state. Trivalent actinides and fission products remain predominantly in the raffinate, although a significant Tc fraction will co-extract with the U and Pu

  1. The effect of electroacupuncture on depressive rats's behavior induced by intracerebroventricular injection of L-AAA%电针对侧脑室注射L-AAA致抑郁模型大鼠行为学的影响

    Institute of Scientific and Technical Information of China (English)

    孙健; 姜硕; 刘健华; 符文彬; 蒋丽; 黄彬; 肖遥; 李富铭

    2013-01-01

    目的 观察侧脑室注射L-α-aminoadipic acid(L-AAA)对大鼠抑郁样行为的改变及电针的治疗效应.方法 将45只雌性SD大鼠随机分为空白组、侧脑室注射生理盐水组(侧注NS组)、侧脑室注射L-AAA组(侧注LAAA组)、针刺组、利鲁唑灌胃组(药物组),每组9只;观察侧脑室注射L-AAA后大鼠行为学的变化及电针、药物对侧脑室注射L-AAA模型大鼠抑郁样行为的改善作用.结果 侧脑室注射L-AAA后大鼠出现体重下降、新环境抑制饮食时间延长、糖水消耗率下降和运动量减少等类抑郁症状,与空白组及侧注NS组比较具有显著性差异(P<0.01);经电针、药物治疗后大鼠的类抑郁症状明显减轻,与侧注射LAAA组比较具有显著性差异(P<0.01),基本恢复至正常水平(空白组、侧注NS组).结论 侧脑室注射LAAA后可以诱发大鼠的类抑郁症状,而电针可以明显改善此类症状,且疗效与利鲁唑相似.据此推测电针抗抑郁的机制可能与促进星形胶质细胞修复有关.%Objective To observe the effect of intracerebroventricular injection of L- α -aminoadipic acid ( L-AAA) inducing to depressive behavior in rats and the effect of electroacupuncture ( EA) on it. Methods 45 female SD rats were randomly distributed into blank, intracerebroventricular injection of normal saline ( NS) , intracerebroventricular injection of L-AAA ( LAAA) , acupuncture, riluzole gavage ( drug) groups, 9 rats for each. The behavior changes of rats after intracerebroventricular infusion of L-AAA and the effect of electroacupuncture , drugs on these depression-like behavior of intracerebroventricular injection of L-AAA model rats were observed. Results ( 1 ) There were depressive symptoms in rats after intracerebroventricular injection of L-AAA, including weight loss, new environment restrain food time prolonged, sucrose consumption rate decreasing and exercise loss, the difference was statistically significant compared

  2. 基于AAA服务器策略的企业网动态安全访问的实现%Implementation of Dynamic Security Access of Entranet Based on AAA Server Policy

    Institute of Scientific and Technical Information of China (English)

    彭亚发; 洪晓峰

    2013-01-01

    the AAA server strategy can achieve the function of IEEE802.1X includes authorization, authentication and statistics. In this paper, through the application of AAA server in enterprise network to solve different internal server to meet different user access requirements. Through the AAA server strategy not only can effectively control the unauthorized user access to internal corporate servers, pose a threat to the enterprise information security, but also can avoid the network device configuration is very complex ACL access rules cause equipment burden reduced performance of the phenomenon, to improve the efficiency of enterprise network and realize the dynamic security of network users enterprise network access.%利用AAA服务器策略可以实现IEEE802.1X的授权、认证和统计功能,通过在企业网络中应用AAA服务器策略解决企业内部不同的服务器满足不同用户访问的需求,通过AAA服务器策略不但能够有效控制未经授权的用户访问企业内部服务器,对企业信息安全造成威胁,而且能够避免在网络设备上配置相当复杂的ACL访问规则造成设备负担过重而性能降低的现象,从而提高了企业网络的运行效率并实现网络用户对企业网络的动态安全访问。

  3. AAA Foundation for Traffic Safety

    Science.gov (United States)

    ... Powerful In Driving Restrictions: Study - Nature World News Pokemon Go and distracted driving | WWLP.com - wwlp.com The ... for earlier curfew for teen drivers - WGRZ.com Pokémon GO continues to raise distracted driving worries - Wisconsin Radio ...

  4. Two AAA Family Peroxins, PpPex1p and PpPex6p, Interact with Each Other in an ATP-Dependent Manner and Are Associated with Different Subcellular Membranous Structures Distinct from Peroxisomes

    OpenAIRE

    Faber, Klaas Nico; Heyman, John A.; Subramani, Suresh

    1998-01-01

    Two peroxins of the AAA family, PpPex1p and PpPex6p, are required for peroxisome biogenesis in the yeast Pichia pastoris. Cells from the corresponding deletion strains (PpΔpex1 and PpΔpex6) contain only small vesicular remnants of peroxisomes, the bulk of peroxisomal matrix proteins is mislocalized to the cytosol, and these cells cannot grow in peroxisome-requiring media (J. A. Heyman, E. Monosov, and S. Subramani, J. Cell Biol. 127:1259–1273, 1994; A. P. Spong and S. Subramani, J. Cell Biol....

  5. The Logos of A!A"A? of The Aalto University School of Art and Design%从A!A”A?三款校徽标识所悟到的

    Institute of Scientific and Technical Information of China (English)

    黄迅

    2012-01-01

    去年暑假期间。我们一行十几人,在广东工业大学艺术设计学院院长方海教授的带领下,开始了为期十二天对芬兰阿尔托大学的考察学习。%The paper records the writer's visit to The Aalto University School of Art and Design in Finland, especially how the writer got confused about the Iogos of A!A"A? of The Aalto University School of Art and Design at first and finally figured it out.

  6. Akçalar (Musa Deresi Azot Ve Fosfor Yüklerinin Mevsimsel Değişimi Ve Uluabat Gölü’ne Etkisi

    Directory of Open Access Journals (Sweden)

    Aslıhan KÂTİP

    2013-12-01

    Full Text Available Uluslararası öneme sahip, Ramsar sözleşmesine tabi olan Uluabat Gölü’ne kıyısı bulunan Akçalar Beldesi’nin evsel atıksuları ve beldede bulunan sanayi kuruluşlarından ve mezbahalardan kaynaklanan atıksular Akçalar Deresi (Musa Deresi yoluyla Uluabat Gölü’ne deşarj olmaktadır. Atıksular gölün özellikle doğu kısmını kirleterek su kalitesini düşürmektedir. Bu çalışmada Akçalar Deresi’nin 2008-2009 yılı boyunca debi ölçümleri yapılarak, TN, NH4-N, NO3-N, TP ve PO4-P konsantrasyonları ve kirlilik yükleri incelenmiştir. Buna göre TN, NH4-N, NO3-N, TP ve PO4-P yıllık toplam kirlilik yükleri sırasıyla 22,45 ton/yıl, 3,14 ton/yıl, 3,04 ton/yıl, 0,58 ton/yıl ve 0,46 ton/yıl olarak hesaplanmıştır. Kirlilik yüklerinin aylık değerlendirilmesine göre en yüksek değerler Mart Ayı’nda belirlenmiştir. Akçalar Deresi ve Uluabat Gölü’ndeki kirliliği önlemek ve kirlik yüklerini azaltmak amacıyla en kısa sürede evsel ve endüstriyel atıksular için arıtma tesisleri yapılmalı, tarımsal uygulamalarda gübre ve pestisit kısıtlaması getirilmeli ve Avrupa Birliğine uyum sürecinde Su Çerçeve Direktifi için gerekli çalışmalar başlatılmalıdır.

  7. Expression of ACO1, ERS1 and ERF1 genes in harvested bananas in relation to heat-induced defense against Colletotrichum musae.

    Science.gov (United States)

    Zhu, Xiangfei; Wang, Aiping; Zhu, Shijiang; Zhang, Lubin

    2011-09-15

    The aim of this study was to investigate the connection between heat-induced ethylene signal changes and enhanced disease resistance. Heat enhanced ripening and elevated MaACO1 expression in naturally ripened bananas (NRB), while it delayed ripening and reduced MaACO1expression in the ethephon-treated bananas (ETB). However, in both cases, heat reduced lesion sizes infected by Colletotrichum musae. This indicates that heat-induced disease resistance in bananas was independent of ripening rate. The expression of MaERS1 gene was inhibited by heat treatment in both NRB and ETB, implying that heat as a physical signal could be sensed by banana fruits through the inhibition of ethylene receptor gene expression. The intensity of MaERF1 transcript signals was elevated in heated bananas, suggesting that the enhanced accumulation of MaERF1 transcript following heat treatment could play an important role in activation of the defense system. In ETB, inhibition of JA biosynthesis by application of IBU down-regulated the expression of MaERF and significantly weakened disease resistance, suggesting involvement of endogenous JA in induction of the gene expression, which was reconfirmed by the fact that exposure to exogenous MeJA following the combination of heat plus IBU treatment restored part of the gene expression. On the other hand, in NRB, application of IBU elevated level of MaERF1 expression at 24h and enhanced disease resistance, suggesting that, when banana was not exposed to ethephon, the expression of MaERF1 gene was not JA dependent, which was verified by the fact that MeJA application did not enhance MaERF1 gene expression. In conclusion, heat-induced disease resistance in harvested bananas could involve down-regulation of MaERS1 expression and up-regulation of MaERF1 expression and JA pathway could be involved in heat activation of the defense system in bananas exposed to ethephon. PMID:21511361

  8. Ameliorative potentials of cocoyam (Colocasia esculenta L.) and unripe plantain (Musa paradisiacal L.) on renal and liver growth in streptozotocin induced diabetic rats

    Institute of Scientific and Technical Information of China (English)

    Eleazu CO; Iroaganachi M; Eleazu KC

    2013-01-01

    Objective:To investigate the ameliorating potentials of cocoyam(Colocasia esculenta(C. esculenta)L.) and unripe plantain(Musa paradisiacae(M. paradisiacae)L.) incorporated feeds on renal and liver growth ofSTZ induced rats.Method:The blood glucose level of all the rats was measured with a glucometer, the protein and glucose levels in the urine samples of the rats were determined using urine assay strips while the specific gravity of the urine samples of all the rats was determined with a urinometer.The assay of the proximate, phytochemical, mineral composition as well as screening for antioxidant activity of the test feeds was carried out using standard techniques.Results:The administration of the test feeds to the diabetic rats in58.75% and38.13% decreases in their hyperglycemia with a corresponding amelioration of their elevated urinary protein, glucose, specific gravity as well as renal and kidney growths.Administration of the cocoyam incorporated feeds to the diabetic rats of group4, resulted in2.71% increase in body weight with a corresponding19.52% increase in growth rate unlike the diabetic rats of group 5, administered unripe plantain feed that had5.12% decrease in weight with a corresponding 29.52% decrease in growth rate but higher than the diabetic control rats that recorded28.69% and 29.46% decreases in body weights with a corresponding248.9% and250.14% decreases in growth rates.Analysis revealed that the test feeds contained low quantities of moisture but significant quantities of crude fibre, proteins, lipids, carbohydrates, ash, alkaloids, flavonoids, saponins, tannins, calcium, magnesium, potassium, iron, zinc, phosphorous as well as considerable amount of energy.In addition, the cocoyam incorporated feeds contained higher quantities of flavonoids, saponin, tannin,Ca,Mg,Fe,Zn,K,P, crude fibre as well as antioxidant activity but lower quantities of alkaloids than the unripe plantain feed.Conclusion:The use of cocoyam and unripe plantain flours in the

  9. Adsorption Study on Moringa Oleifera Seeds and Musa Cavendish as Natural Water Purification Agents for Removal of Lead, Nickel and Cadmium from Drinking Water

    Science.gov (United States)

    Aziz, N. A. A.; Jayasuriya, N.; Fan, L.

    2016-07-01

    The effectiveness of plant based materials Moringa oleifera (Moringa) seeds and Musa cavendish (banana peel) for removing heavy metals namely lead (Pb), nickel (Ni) and cadmium (Cd) from contaminated groundwater was studied. Tests were carried out with individual and combined biomass at neutral pH condition on synthetic groundwater samples. The optimum biomass doses were determined as 200 mg/L for single biomass and 400 mg/L (in the ratio of 200 mg/L: 200 mg/L) for combined biomasses and used for adsorption isotherm studies with contact time of 30 minutes. Results showed that combined biomasses was able to met the Pb, Ni and Cd WHO standards from higher Pb, Ni and Cd initial concentrations which were up to 40 µg/L, 50 µg/L 9 µg/L, respectively compared to individual biomass of Moringa seed and banana peel. Moringa seeds exhibited the highest removal of Pb (81%) while the combined biomasses was most effective in removing Ni (74%) and Cd (97%) over wider their initial concentration ranges. The experimental data were linearized with Langmuir and Freundlich adsorption isotherm models. Freundlich model described the Pb adsorption better than the Langmuir model for all the tested biomasses. However, the Langmuir model fit better with the experimental data of Ni adsorption by Moringa seeds. Both models showed negligible differences in the coefficient of determination (R2) when applied for Ni and Cd adsorption on banana peel and combined biomasses, suggesting that there were multiple layers on the biomass interacting with the metals. Chemisorption is suggested to be involved in Pb adsorption for all tested biomasses as the value of nF calculated was lower than one. This type of adsorption could explain the phenomenon of different behavior of Pb removal and the higher Pb adsorption capacity (represented by KF values) compared to Ni and Cd. The study demonstrates that Moringa seeds, banana peel and their combination have the potential to be used as a natural alternative

  10. DILEMAS NA TRADUÇÃO DE MUSE & DRUDGE (“MUSA E MULA”, DE HARRYETTE MULLEN: NEGRITUDE, DISSONÂNCIA E MISCIGENAÇÃO

    Directory of Open Access Journals (Sweden)

    Lauro Maia Amorim

    2014-10-01

    Full Text Available O trabalho discute um projeto de tradução, para o português brasileiro, de um dos poemas da obra Muse & Drudge (“Musa e Mula” da premiada poeta afro-americana Harryette Mullen. Em Muse & Drudge, Mullen entrelaça crítica cultural com humor, lirismo e trocadilhos que desdobram as fronteiras entre identidade cultural e racial, além de redimensionar os limites da oposição entre cultura popular e erudita. Pretende-se discutir em que medida a tradução proposta produz outras relações intertextuais que culminam com efeitos de sentido inesperados. Pretende-se também avaliar de que modo os interstícios desse “inesperado” revela o “encontro” entre as chamadas categorias raciais black e white, dominantes na cultura norte-americana, e a noção de miscigenação e “democracia racial” no Brasil.Abstract: This paper aims to discuss a project of translating part of the work Muse & Drudge, by the award-winning African-American poet Harryette Mullen, into Brazilian Portuguese, with focus on a single poem. In Muse & Drudge Mullen combines cultural critique with humor, lyricism and punning, which has unfolded the frontiers between cultural and racial identity, and has put into question the opposition between popular and high culture. This work analyzes to which extent the proposed translation produces a new set of intertextual relations that might culminate in “unexpected” meanings. It is a goal to understand how the effects of such “unexpected” meanings reveal the “encounter” between the so-called racial “black/white” dichotomy, predominant in the US culture, and the notion of “miscegenation” and “racial democracy” in Brazil.Keywords: Translation; African-American poetry; miscegenation; dilemmas.Lauro Maia Amorim é professor Substituto de Teoria da Tradução da Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP.

  11. The Use of Alternate Ligno-cellulosic Raw Materials Banana (Musa sapientum Ankara (Calotropis procera and Pineapple (Ananas comosus in Handmade Paper & their Blending with Waste Paper.

    Directory of Open Access Journals (Sweden)

    Atul Kumar

    2013-09-01

    Full Text Available The studies were made to established suitability of lingo-cellulosic raw materials namely leaf fibreBanana (Musa Sapientum, bast fibre Ankara (Calotropis Procera, & leaf fibre Pineapple (Ananas Comosus for making pulps for handmade paper industry. This should help in providing a cost effective, good quality cellulosic raw material as an alternate to cost prohibitive traditionally used cotton hosiery waste traditionally used for manufacturing good quality handmade paper & it’s products. This will help in improving the cost economics & competitiveness of the Indian paper industry in the global market besides addressing the problems of environment & global warming. The aim of the research was to study the extraction, morphology, chemical composition and pulping of these fibres and relate these properties to the composite properties obtained with these fibres as reinforcement with short fibres viz. waste paper. For the pulp production to be feasible it is essential to use suitable pulping methods, which maximize the yield of pulp and introduce as low damage as possible to the fibres. The different pulping methods were applied to these fibre to get optimized strength properties papers. The Studies thus carried out provide useful information about the nature of these raw materials, suitable pulping & bleaching process to produce an eco-friendly handmade paper and converted products. The research work provides a good quality cost effective lingo-cellulosic raw material for handmade paper industries with a possibility of replacement of the expensive and traditionally used cotton hosiery waste.The paper thus produced using environmental friendly pulping and bleaching process is characterized for its strength properties like tensile, tear, bursting, folding endurance and other parameters. The effluents generated from pulping and bleaching of above ligno-cellulosic waste materials were characterized for various pollution parameters like Residual Alkali

  12. Efecto de la edad de cosecha en las características poscosecha del plátano Dominico-Hartón (Musa AAB Simmonds

    Directory of Open Access Journals (Sweden)

    Luis Fernando Mejía-Gutiérrez

    2012-12-01

    Full Text Available En el departamento de Caldas, Colombia, se realizó la caracterización física, química y fisiológica durante las fases de cosecha y poscosecha del plátano Dominico Hartón (Musa AAB Simmonds cultivado en el municipio de Belalcázar. La investigación se hizo con frutos de 14, 16 y 18 semanas después de la floración. Cada 2 días y hasta senescencia se evaluaron los cambios de peso, firmeza, parámetros de color L*a*b*, relación pulpa/cáscara, índice de madurez, pH, humedad, almidones, índice de respiración y tasa de etileno. En este trabajo se utilizó un diseño longitudinal de medidas repetidas, balanceado, con un factor en tres niveles. El factor evaluado fue el tiempo de cosecha y las variables de respuesta fueron los parámetros fisicoquímicos. Los resultados mostraron variación en peso cercanas a 7%, el parámetro de color tinte varió entre -61.46 y 86.74, el croma entre 26.31 y 37.11 y el DE entre 1.4603 y 8.360, el pH varió entre 6.2 y 3.98, una relación pulpa/cáscara entre 2.53 y 4.11, la relación °brix/ acidez entre 11.36 y 26.2, la humedad entre 60.56% y 56%, el almidón entre 51.7% y 67% de MS, la tasa de producción de CO2 entre 3.4 y 8.9 g/kg por hora, el etileno entre 24 y 225 µlt/kg por hora. Los parámetros de maduración mostraron comportamiento climatérico y los días a maduración total fueron de 9, 14 y 21 para los frutos de 18, 16 y 14 semanas desde floración.

  13. Caracterización fisicoquímica y fisiológica del proceso de maduración de plátano Hartón (musa AAB Simmonds en dos sistemas de producción Physical-chemist and physiologic characterization in the Harton plantain ripening process (Musa AAB Simmonds in organic and conventional production systems

    Directory of Open Access Journals (Sweden)

    José Luis Barrera V

    2010-01-01

    Full Text Available En el Laboratorio de Análisis de Alimentos de la Universidad de Córdoba (Colombia se realizó la caracterización fisicoquímica y fisiológica del proceso de maduración del plátano Hartón (Musa AAB Simmonds cultivado en los sistemas de producción orgánico y convencional. Para el estudio se utilizó un diseño completamente al azar con arreglo factorial 2 x 5: dos sistemas de producción y cinco estados de maduración. Los análisis realizados fueron físicos: resistencia, días de maduración; químicos: pH, °Brix, azúcares totales y reductores y almidón; y fisiológicos: tasa de respiración a temperatura ambiente. Los resultados obtenidos mostraron que entre los tratamientos (orgánico y convencional durante el proceso de maduración se observaron diferencias significativas en las variables resistencia, °Brix, pH, acidez; por el contrario las variables azúcares reductores, azúcares totales y almidón tuvieron un comportamiento similar. El índice de respiración en plátano orgánico alcanzó el pico climatérico a los 14 días, y el convencional a los 7, indicando que el primero presenta un mejor comportamiento poscosecha facilitando su comercialización.The physical-chemist and physiologic portrayal in the Harton plantain ripening process (Musa AAB Simmonds in the system of organic production and conventional in the Cordoba - Colombia department, was carried out in the food analysis laboratory of the University of Cordoba, in order to establish the differences physical-chemist and physiologic between the system production already mentioned. To reach the objective was used a design completely at random with factorial repair two per five, with two factors that were the production system and the state of ripening. The analysis carried out were physics (resistance, ripening days; chemist (pH, °Brix, total and reduction sugar, starch and physiologic (respiration rate to environmental temperature. The results obtained show that

  14. Influencia del Tratamiento Osmótico en el Secado de la Banana “Nanica” (Musa cavendishii, L. en Secador de Lecho Fijo Influence of Osmotic Treatment onthe Drying of "Nanica" Bananas (Musa cavendishii, L. in a Fixed Bed Dryer

    Directory of Open Access Journals (Sweden)

    O.C.P. Gaspareto

    2004-01-01

    Full Text Available Se ha estudiado la influencia de la concentración de azúcar (50 y 70 °Brix y de la temperatura (50 y 70 °C en la deshidratación osmótica de la Banana “Nanica” (Musa cavendishii, L.. Para el secado complementario fue empleado un secador de lecho fijo a 60 °C y velocidad del aire 2.0 m/s. Durante el secado se verificó el comportamiento cinético del proceso, usando datos experimentales de los períodos de velocidad decrecientes, obteniéndose curvas de velocidad de secado versus humedad. Basándose en un modelo difusional fueron calculadas las difusividades efectivas, para 50 °Brix/50 °C, 50 °Brix/70 °C, 70 °Brix/50 °C y 70 °Brix/70 °C resultando 9.12x10-10, 9.46x10-10, 1.01x10-09 y 1.05x10-09 m²/s, respectivamente. De los resultados obtenidos, se observó que la difusividad efectiva no estuvo influenciada por la concentración de azúcar y tampoco por la temperatura. Por lo tanto, la mejor condición de pérdida de humedad y ganancia de azúcar fue de 70 °Brix/50 °C.The influence of sugar concentrations (50 and 70 °Brix and temperature (50 and 70 ºC on the osmotic dehydration of "Nanica" Bananas (Musa cavendishii, L., have been studied. For supplementary drying, a fixed bed drier was used at 60 ºC with an air flow of 2.0 m/s. The kinetic behavior of the process was verified during drying using experimental data on the periods of decreasing velocities to obtain curves of drying speed vs humidity. Effective diffusivities were calculated based on a diffusion model for 50 ºBrix/50 ºC, 50 ºBrix/70 ºC, 70 ºBrix/50 ºC and 70 ºBrix/70 ºC, with respective results of 9.12x10-10, 9.46x10-10, 1.01x10-09 and 1.05x10-09 m²/s. It was observed that the effective diffusivities were neither influenced by the sugar concentration nor by the temperature. The best condition of humidity loss and sugar gain was at 70 ºBrix/50º C.

  15. Caracterización fisicoquímica y fisiológica del proceso de maduración de plátano hartón (musa aab simmonds) en dos sistemas de producción

    OpenAIRE

    Cayón S Daniel Gerardo; Arrazola P Guillermo Segundo; Barrera V José Luis

    2010-01-01

    En el Laboratorio de Análisis de Alimentos de la Universidad de Córdoba (Colombia) se realizó la caracterización fisicoquímica y fisiológica del proceso de maduración del plátano Hartón (Musa AAB Simmonds) cultivado en los sistemas de producción orgánico y convencional. Para el estudio se utilizó un diseño completamente al azar con arreglo factorial 2 x 5: dos sistemas de producción y cinco estados de maduración. Los análisis realizados fueron físicos: resistencia, días de maduración; químico...

  16. Production potentielle de bioéthanol, de biométhane et de pellets à partir des déchets de biomasse lignocellulosique du bananier (Musa spp. au Cameroun

    Directory of Open Access Journals (Sweden)

    Kamdem, I.

    2011-01-01

    Full Text Available Potential production of bioethanol, biomethane and wood pellets from lignocellulosic biomass wastes of the banana plant (Musa spp. in Cameroon. Like most African countries who are producers and exporters of banana, Cameroon is facing a major energy deficit. Yet, the country is generating annually about 4,500,000 tons of fresh banana plant lignocellulosic waste biomass matter equivalent to 402,750 tons of dry matter. The dry matter contained about 80,57% organic matter which are not exploited. Under the sustainable development, which is linked to environmental protection, the biotransformation of these residues can potentially produce about 93,800; 92,133; 447,500 tons of bioethanol, biomethane and pellets respectively. The waste transformation could reduce the energy deficit and create jobs opportunities. Productions of this renewable energy or biofuel also constitute a new area which could assure an important source of income for the banana cultivators and the entire country.

  17. CRESCIMENTO E OXIDAÇÃO DE EXPLANTES DE BANANEIRA PRATA (Musa AAB) IN VITRO: IV. CONCENTRAÇÕES DE SAIS, ÁCIDOS ASCÓRBICOS E FREQÜÊNCIA DE SUBCULTIVOS

    OpenAIRE

    UTINO SERGIO; CARNEIRO IRAÍDES FERNANDES; CHAVES LÁZARO JOSÉ

    2001-01-01

    Avaliaram-se diferentes concentrações de sais do meio MS (Murashige & Skoog, 1962), freqüência de subcultivos e adição de ácido ascórbico ao meio de cultura, objetivando o controle da oxidação de explantes de bananeira-'Prata' (Musa AAB) na fase de estabelecimento. Os tratamentos constituíram-se das diluições dos sais do meio MS (100%, 50% e 33,33%), subcultivos (a cada 7 dias, a cada 14 dias e a cada 28 dias) e ácido ascórbico (0 e 25 mg L-1). O delineamento experimental foi inteiramente cas...

  18. High resolution mass spectrometry imaging reveals the occurrence of phenylphenalenone-type compounds in red paracytic stomata and red epidermis tissue of Musa acuminata ssp. zebrina cv. 'Rowe Red'.

    Science.gov (United States)

    Hölscher, Dirk; Fuchser, Jens; Knop, Katrin; Menezes, Riya C; Buerkert, Andreas; Svatoš, Aleš; Schubert, Ulrich S; Schneider, Bernd

    2015-08-01

    The banana epidermis and in particular their stomata are conducive sites for the penetration of pathogenic fungi which can severely limit global banana production. The red pseudostem of the ornamental banana Musa acuminata ssp. zebrina cv. 'Rowe Red' was used to study the chemical constituents of the epidermal cell layer using matrix-free laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometric imaging (LDI-FT-ICR-MSI). The high resolution of this technique allowed phenylphenalenone-type compounds to be located in single plant cells. Some of these secondary metabolites were identified as constitutive compounds and found in specialized epidermal cells in banana pseudostem tissue. Especially the red paracytic stomata revealed higher signal intensities of certain phenylphenalenones than normal epidermis cells. The ease of detection of polycyclic aromatic compounds on the cellular level is discussed with regard to future investigations of plant-pathogen interactions. PMID:26004822

  19. Utilización de la metodología MUSA y su aplicación en el estudio para monitorear periódicamente la satisfacción de estudiantes de la Universidad de los Andes (ESAT

    Directory of Open Access Journals (Sweden)

    Ciro Alberto Amaya Guio

    2014-08-01

    Full Text Available El propósito de este trabajo es aplicar el método MUSA a la información obtenida en las encuestas del estudio para monitorear periódicamente la satisfacción de estudiantes (ESAT, para diagnosticar el nivel de satisfacción actual de los estudiantes de pregrado de la Universidad de los Andes (Colombia e identificar la importancia y el desempeño de cada uno de los aspectos evaluados por la institución. Como parte de los resultados, es posible conocer los elementos que los estudiantes consideran más valiosos dentro de su satisfacción, tomando los resultados emitidos por una muestra significativa de encuestados. Finalmente, el estudio presenta las opciones que requieren mayor atención por parte de la Universidad para mejorar dicha satisfacción.

  20. EVALUATION OF ETHANOL PRODUCTION FROM TWO RECOMBINANT AND A COMMERCIAL STRAINS OF SACCHAROMYCES CEREVISIAE (FUNGI: ASCOMYCOTA IN SUGAR-CANE MOLASSES AND REJECTED-BANANA JUICE FROM URABÁ, COLOMBIA EVALUACIÓN DE LA PRODUCCIÓN DE ETANOL POR DOS CEPAS RECOMBINANTES Y UNA COMERCIAL DE SACCHAROMYCES CEREVISIAE (FUNGI: ASCOMYCOTA EN MELAZA DE CAÑA DE AZÚCAR Y MOSTOS DE BANANO DE RECHAZO DE URABÁ, COLOMBIA

    Directory of Open Access Journals (Sweden)

    Carolina Peña-Serna

    2011-12-01

    Full Text Available The production of bioethanol using Saccharomyces cerevisiae (Fungi: Ascomycota is influenced by sugar concentrations and the fermentation substrate. For that reason, in this study the kinetics of biomass production, residual sugar and ethanol production of four S. cerevisiae strains were evaluated in two fermentation media (sugar-cane molasses and rejected-banana juice at two sugar concentrations (100 and 170 g/l. The Ethanol Red® and GG570- CIBII strains exhibited the greatest ethanol production, with peak values of 119.74 (35 h and 62 g/l (15 h, Yps 0.75 and 0.43 g/g, and Qp 3.42 and 2.61 g/l/h, respectively, at 170 g/l of sugar in the sugar-cane molasses broth. In additional, the GG570-CIBII strain showed an increase of 37.1 g/l ethanol with respect to the control strain.La producción de bioetanol a partir de Saccharomyces cerevisiae (Fungi: Ascomycota está influenciada por la concentración de azúcares y el sustrato de fermentación. Por ello, en este trabajo se evaluaron las cinéticas de producción de biomasa, azúcares residuales y producción de etanol de cuatro cepas de S. cerevisiae en dos medios de fermentación (melaza de caña de azúcar y banano de rechazo a dos concentraciones de azúcares (100 y 170 g/l. Las cepas Ethanol Red® y GG570-CIBII presentaron mayor producción de etanol con pico de producción de 119,74 (35 h y 62 g/l (15 h, Yps 0,75 y 0,43 g/g y Qp 3,42 y 2,61 g/l/h, respectivamente a 170 g/l de azúcares en melaza de caña de azúcar. Adicionalmente, la cepa GG570-CIBII mostró un incremento de 37,1 g/l de etanol con respecto a la cepa control.

  1. EVALUATION OF ETHANOL PRODUCTION FROM TWO RECOMBINANT AND A COMMERCIAL STRAINS OF SACCHAROMYCES CEREVISIAE (FUNGI: ASCOMYCOTA IN SUGAR-CANE MOLASSES AND REJECTED-BANANA JUICE FROM URABÁ (ANTIOQUIA, COLOMBIA EVALUACIÓN DE LA PRODUCCIÓN DE ETANOL POR DOS CEPAS RECOMBINANTES Y UNA COMERCIAL DE SACCHAROMYCES CEREVISIAE (FUNGI: ASCOMYCOTA EN MELAZA DE CAÑA DE AZÚCAR Y MOSTOS DE BANANO DE RECHAZO DE URABÁ (ANTIOQUIA, COLOMBIA

    Directory of Open Access Journals (Sweden)

    Carolina Peña-Serna

    2012-06-01

    Full Text Available The production of bioethanol using Saccharomyces cerevisiae (Fungi: Ascomycota is influenced by sugar concentration and fermentation substrate. For that reason, the kinetics of biomass production, residual sugars, and ethanol production of four of S. cerevisiae strains were evaluated in two different fermentation media (sugar-cane molasses and rejected-banana juice at two different sugar concentrations (100 and 170 g/l. The EthanolRed® and GG570-CIBII strains exhibited the greatest ethanol production, with peak values of 119.74 (35 h and 62 g/l (15 h, Yps 0.75 and 0.43 g/g, and Qp 3.42 and 2.61 g/l/h, respectively, at 170 g/l of sugar in sugar-cane molasses broth. Additionally, the strain GG570-CIBII showed an increase of 37.1 g/l ethanol with respect to the control strain.La producción de bioetanol a partir de Saccharomyces cerevisiae (Fungi: Ascomycota está influenciada por la concentración de azúcares y el sustrato de fermentación. Por ello en este trabajo se evaluaron las cinéticas de producción de biomasa, azúcares residuales y producción de etanol de cuatro cepas de S. cerevisiae en dos medios de fermentación (melaza de caña de azúcar y banano de rechazo a dos concentraciones de azúcares (100 y 170 g/l. Las cepas EthanolRed® y GG570-CIBII presentaron mayor producción de etanol con pico de producción de 119,74 (35 h y 62 g/l (15 h, Yps 0,75 y 0,43 g/g y, Qp 3,42 y 2,61 g/l/h, respectivamente a 170 g/l de azúcares en melaza de caña de azúcar. Adicionalmente, la cepa GG570-CIBII mostró un incremento de 37,1 g/l de etanol con respecto a la cepa control.

  2. Efeito da interação entre carvão ativado e N6-benzilaminopurina na propagação in vitro de bananeira, cv. Grand Naine (AAA Interaction effect between activated charcoal and N6-benzylaminopurine in the in vitro propagation of banana, cultivar Grand Naine (AAA

    Directory of Open Access Journals (Sweden)

    Frederico Henrique da Silva Costa

    2006-08-01

    Full Text Available O carvão ativado possui a propriedade de adsorver os compostos fenólicos liberados pela oxidação dos tecidos lesionados durante o cultivo in vitro. O objetivo deste trabalho foi avaliar os efeitos da interação entre o carvão ativado e diferentes concentrações de N6-benzilaminopurina (BAP na multiplicação in vitro da bananeira, cv. Grande Naine (AAA. O meio de cultura utilizado foi o MS, solidificado com 5 g.L-1 de ágar. O cultivo foi mantido em sala de crescimento a 25±2ºC, fotoperíodo de 16 horas e intensidade luminosa de 30 mmol.m-2s-1. Foram avaliadas a presença e a ausência de carvão ativado (0 e 3 g.L-1 e quatro concentrações de BAP (0; 2; 4 e 6 mg.L-1 no meio de cultura. O delineamento foi inteiramente casualizado, com cinco repetições, em um sistema fatorial 2x4. Os explantes foram avaliados a cada 30 dias, por um período de quatro subcultivos. Após cada subcultivo, o comprimento de brotações, a taxa de multiplicação, o vigor, o nível de oxidação das brotações emitidas e o número de raízes formadas foram avaliados. Independentemente das concentrações de BAP, o carvão ativado influenciou significativamente em todas as variáveis analisadas. De maneira geral, a adição de carvão ativado afetou negativamente a taxa de multiplicação, embora tenha melhorado o vigor e o número de raízes e diminuído a oxidação dos explantes. Na ausência de carvão ativado, o BAP proporcionou as maiores taxas de multiplicação das brotações.Active charcoal has the capacity to fix phenolic compounds released by the oxidation of manipulated tissues during in vitro cultivation. The present work objective was to evaluate the interaction effects between active charcoal, combined to different BAP concentrations in the in vitro banana propagation, cv. Grand Nain (AAA. The culture medium used was the MS, solidified with 5 g.L-1 agar. Cultures were kept in a growth room at 25 ± 2ºC with a photoperiod of 16 h at 30 mmol

  3. El autor y su musa

    Directory of Open Access Journals (Sweden)

    Dardo Scavino

    2006-04-01

    Full Text Available …la paradoja es la pasión del pensamiento, y el pensador sin paradoja es comoel amante sin pasión : un mediocre sujeto.Sören Kierkegaard1/ OxímoronPerelman y Olbrechts-Tyteca proponían inscribir el paradoxisme–figura que englobaría la paradoja, la antilogía y el oxímoron– entre los “razonamientos por disociación”, un recurso muy corriente, según ellos, “en los escritos de filósofos obligados a redefinir las nociones del lenguaje común para adaptarlas a su propio sistema” (Perelman : 1958, p. ...

  4. Caracterización química de macroelementos en suelos cultivados con plátano (Musa AAB Simmonds en el departamento de Córdoba, Colombia Chemical characterization of macroelements in soils cultivated with Harton plantain (Musa AAB Simmonds in the department of Córdoba, Colombia

    Directory of Open Access Journals (Sweden)

    Enrique Combatt-Caballero

    2012-04-01

    Full Text Available Las recomendaciones de fertilización para el cultivo de plátano (Musa AAB Simmonds deben estar basadas en las características químicas y el potencial de producción de los suelos. El objetivo de este trabajo fue evaluar las características químicas de macro-elementos presentes en suelos utilizados en la producción de plátano en el departamento de Córdoba, Colombia. Las evaluaciones se hicieron en diez fincas por cada uno de cuatro municipios productores de plátano en el Alto Sinú (Valencia y Tierralta y la región costanera (San Bernardo del Viento y Moñitos. Los parámetros determinados en el suelo fueron: pH, materia orgánica (M.O., P, S, Ca, Mg, Na y K, según metodologías químicas propuestas por Instituto Geográfico Agustín Codazi (IGAC. Los datos obtenidos fueron sometidos a análisis de varianza y pruebas de comparación de medias (Tukey. En los resultados analíticos se encontró que los suelos presentan reacción moderadamente ácida con pH Fertilizer recommendations for hartón plantain cropping should be done by interpreting the chemical characteristics of soils to be reliable from the technical point of view and principally based in technical adjustments in relation to the nutritional potential of soils. The aim of this study was to evaluate the chemical characteristics of macroelements in soil in 40 farms cultivated with hartón plantain in the department of Córdoba, Colombia. The parameters measured were: pH, organic matter (O.M., P, S, Ca, Mg, Na, K, according to the analytical procedures proposed by Geographic Institute Agustín Codaz (IGAC. Analysis of variance and mean comparison tests (Tukey to the data were performed. The analytical results showed that the soils had a moderately acidic reaction, with pH lower than 6 on the High Sinú zone and slightly alkaline with pH 6.5 in the coastal zone. The organic matter content was less than 2.1%, P between 16.4 and 35.3 mg kg-1 and S deficient with values less than

  5. Practice of Creating National AAA Safe and Civilized Demonstration Site in North Huaqiang Metro Station%华强北地铁创建国家级AAA安全文明示范工地实践

    Institute of Scientific and Technical Information of China (English)

    徐新; 吴晓斌

    2015-01-01

    创建国家级AAA安全文明示范工地,提高地铁工程建设的形象,打造精品工程,增强企业的竞争力,提高安全意识和管理水平,交流先进管理经验,抓好工程整体推进及成本投入控制。采取“压噪音、抑扬尘、绝泥水、靓围挡”的文明施工总要求,减少对周边居民和商铺产生的影响,严格落实质量安全文明生产各项规定并形成长效机制,为把华强北建设成国家AAA级安全文明标准化诚信工地奠定了坚实的基础。%This paper tries to create national AAA safe and civilized demonstration site, improve the image of subway construction, create fine works, enhance the competitiveness of enterprises, improve safety awareness and management level, exchange advanced management experience, and grasp the overall progress and cost investment control of project. This paper takes the general requirements of civil construction of "noise reduction, dust suppression, never muddy, good enclosure" to reduce the impact on nearby residents and shops, strictly implements the provisions of safety and civilized production quality and forms long-term mechanism, which has laid a solid foundation for the construction of national AAA safe and civilized demonstration site of north Huaqiang metro station.

  6. EFFECT OF SOME SYSTEMIC DRUGS IN THE PREVENTIVE CONTROL OF THE MAL-DO-PANAMÁ (Fusarium oxysporum f.sp. cubense (EF. SN. & H. OF THE BANANA TREE (Musa sapientum L. EFEITOS DE ALGUMAS DROGAS SISTÊMICAS NO CONTROLE PREVENTIVO DO MAL DO PANAMÁ (Fusarim oxysporum f. sp. Cubense (Ef. Sn. & H. DA BANANEIRA (Musa sapientum L.

    Directory of Open Access Journals (Sweden)

    Yvo de Carvalho

    2007-09-01

    Full Text Available

    With the objective of studying the effect of some systemic drugs on the fungus, Fusarium oxysporim f. sp. cubense (Ef. Sn. & H. , which attacks the rootstalk tissue of the cultivated ‘Maçã’ banana (Musa sapientum L. an experiment was set up in virgin “cerrado” soil , which had been previously covered with natural vegetation. The effects of Benlate (benomyl and Plantvax (oxamyl, both in doses of 0.1, 0.2, and 0.5%, as well as Mycostatin (nistatina in doses of 0.02, 0.05, and 0.1%, and combination of Mycostatin plus soluble Neantina (chloride of metoxi-etil mercurio at 3.7% in doses of 0.02 + 0.15%, 0.05 + 0.3%, and 0.1 + 0.5%, were tested by immersing the infected roostalks in the solutions for a ten-minute period. The rootstalks used had been naturally infected by the fungus. The experiment design used was randomized blocks with four (4 repetitions of twenty-six (26 treatments. Each parcel was composed of four (4 plants. The results observed in February of 1974 revealed an indiscriminate incidence of the ";Mal do Panamá” on the plants of all treatments, demonstrating that none of them was sufficiently effective in erradicating the pathogen established in the rootstalk tissues. It is suggested, because of root damage and the subsequent inadequate penetration by the fungicides, that this lack of absorption was responsible for the unsuccessful attempts at controlling the disease. It was concluded that the control of this disease is neither possible nor viable under the conditions in which the experiment was executed, using the above-mentioned method of fungicide application and preparation of the rootstalk. Changes in procedure are recommended for future experiments.

    Com o objetivo de estudar o efeito de algumas drogas sistêmicas sobre o fungo Fusarium oxysporim f. sp. cubense (Ef. Sn. & H

  7. Avances de la fruticultura en México

    OpenAIRE

    Angel Villegas Monter; Antonio Mora Aguilera

    2011-01-01

    Las estadísticas oficiales informaron que en 2008 se cultivaron 264 944 ha con valor de la producción de $ 14 741 millones de pesos, en 20 frutales de clima templado; mientras que, en 35 tropicales y subtropicales fue de 1 822748 ha con valor de $ 43 463 millones de pesos. De los 55 frutales, sólo naranjo (Citrus sinensis), mango (Mangifera indica), Aguacate (Persea americana), limón mexicano (C. aurantifolia), banano (Musa acuminata), lima persa (C. latifolia) y manzano (Malus domestica) se ...

  8. Caracterización química de macroelementos en suelos cultivados con plátano (Musa AAB Simmonds en el departamento de Córdoba, Colombia

    Directory of Open Access Journals (Sweden)

    Combatt Caballero Enrique

    2012-11-01

    Full Text Available Las recomendaciones de fertilización para el cultivo de plátano (Musa AAB Simmonds deben estar basadas en las características químicas y el potencial de producción de los suelos. El objetivo de este trabajo fue evaluar las características químicas de macro-elementos presentes en suelos utilizados en la producción de plátano en el departamento de Córdoba, Colombia. Las evaluaciones se hicieron en diez fincas por cada uno de cuatro municipios productores de plãtano en el Alto Sinü (Valencia y Tie- rralta y la región costanera (San Bernardo del Viento y Moñitos. Los parámetros determinados en el suelo fueron: pH, materia orgánica (M.O., P, S, Ca, Mg, Na y K, según metodologías químicas propues- tas por Instituto Geogrãfico Agustin Codazi (IGAC. Los datos obtenidos %ueron sometidos a anãlisis de varianza y pruebas de comparación de medias (Tukey. En los resultados analíticos se encontró que los suelos presentan reacción moderadamente ãcida con pH + / en la zona del Alto Sinü7 a ligeramente alcalina con pH de /.8 en la zona costanera. Los contenidos de 9.;. son + 2.=>7 el P Faria entre =/.G y I8.I mgJQg y el S es deficiente con Falores + =I.8 mgJQg en todas las fincas. Los contenidos de Ca7 Mg y K son altos, pero con tendencia a antagonismos iónicos de Ca con respecto a K por las amplias relaciones catiónicas encontradas.

  9. Caracterización fisicoquímica y fisiológica del proceso de maduración de plátano Hartón (musa AAB Simmonds en dos sistemas de producción

    Directory of Open Access Journals (Sweden)

    Cayón S Daniel Gerardo

    2010-03-01

    Full Text Available En el Laboratorio de Análisis de Alimentos de la Universidad de Córdoba (Colombia se realizó la caracterización fisicoquímica y fisiológica del proceso de maduración del plátano Hartón (Musa AAB Simmonds cultivado en los sistemas de producción orgánico y convencional. Para el estudio se utilizó un diseño completamente al azar con arreglo factorial 2 x 5: dos sistemas de producción y cinco estados de maduración. Los análisis realizados fueron físicos: resistencia, días de maduración; químicos: pH, °Brix, azúcares totales y reductores y almidón; y fisiológicos: tasa de respiración a temperatura ambiente. Los resultados obtenidos mostraron que entre los tratamientos (orgánico y convencional durante el proceso de maduración se observaron diferencias significativas en las variables resistencia, °Brix, pH, acidez; por el contrario las variables azúcares reductores, azúcares totales y almidón tuvieron un comportamiento similar. El índice de respiración en plátano orgánico alcanzó el pico climatérico a los 14 días, y el convencional a los 7, indicando que el primero presenta un mejor comportamiento poscosecha facilitando su comercialización.

  10. Caracterización fisicoquímica y fisiológica del proceso de maduración de plátano Hartón (musa AAB Simmonds en dos sistemas de producción

    Directory of Open Access Journals (Sweden)

    José Luis Barrera V

    2010-01-01

    Full Text Available En el Laboratorio de Análisis de Alimentos de la Universidad de Córdoba (Colombia se realizó la caracterización fisicoquímica y fisiológica del proceso de maduración del plátano Hartón (Musa AAB Simmonds cultivado en los sistemas de producción orgánico y convencional. Para el estudio se utilizó un diseño completamente al azar con arreglo factorial 2 x 5: dos sistemas de producción y cinco estados de maduración. Los análisis realizados fueron físicos: resistencia, días de maduración; químicos: pH, °Brix, azúcares totales y reductores y almidón; y fisiológicos: tasa de respiración a temperatura ambiente. Los resultados obtenidos mostraron que entre los tratamientos (orgánico y convencional durante el proceso de maduración se observaron diferencias significativas en las variables resistencia, °Brix, pH, acidez; por el contrario las variables azúcares reductores, azúcares totales y almidón tuvieron un comportamiento similar. El índice de respiración en plátano orgánico alcanzó el pico climatérico a los 14 días, y el convencional a los 7, indicando que el primero presenta un mejor comportamiento poscosecha facilitando su comercialización.

  11. Reducción de poblaciones de Ralstonia solanacearum raza 2 (Smith en plátano (Musa AAB Simmonds con aplicación de extractos de Trichoderma sp. (Alexopoulus y Mims y bacterias antagonistas

    Directory of Open Access Journals (Sweden)

    Germán Ceballos

    2014-01-01

    Full Text Available El moko, enfermedad causada por Ralstonia solanacearum raza 2, afecta la producción de plátano (Musa AAB Simmonds en Colombia, ocasionando pérdidas hasta de 100%. In vitro, se evaluó el efecto de filtrados crudos de cepas de Trichoderma sp. y dos productos comerciales como posibles reductores de poblaciones de R. solanacearum. Para cada cepa de Trichoderma spp., se empleó filtrado (40% en solución acuosa y para R. solanacearum se utilizó una dilución de 10-4 por caja de petri, en un diseño completamente al azar con tres repeticiones para determinar el número de unidades formadoras de colonia de R. solanacearum. En invernadero, se aplicaron suspensiones de T. harzianum (Agroguard®, T. viride 14PDA3A y Ecoterra® en plantas de plátano Dominico Hartón y 30 días después se inoculó R. solanacearum en un diseño de bloques completos al azar con cuatro repeticiones. La severidad del ataque de R. solanacearum se evaluó utilizando una escala de síntomas foliares de 0 a 6. In vitro, los extractos crudos de dos cepas y dos productos comerciales de Trichoderma spp. inhibieron el 100% de R. solanacearum. Los tratamientos con T. viride y Ecoterra® mostraron bajos niveles de control de R. solanacearum en plantas (0.63 y 1.88, respectivamente.

  12. Aplicação de ácido giberélico (GA3 e anatomia da epiderme foliar visando à detecção de variantes somaclonais de bananeira Musa sp. Colla cv. Prata-anã (Musaceae Gibberellic acid (GA3 and anatomy of the leaf epidermises to detect somaclonal variants of the banana tree Musa sp. Colla cv. Prata-anã (Musaceae

    Directory of Open Access Journals (Sweden)

    Janaína de Oliveira Costa Silva

    2010-03-01

    Full Text Available A micropropagação de indivíduos do gênero Musa vem sendo aplicada em larga escala para obtenção de mudas de qualidade fitossanitária. O cultivo in vitro pode levar a ocorrência de variação somaclonal que pode acarretar em prejuízos aos produtores. Atualmente, a diferenciação das plantas variantes somaclonais é possível somente quando as mesmas encontram-se em condições de campo, mediante crescimento exagerado, conhecido como gigantismo. Mutantes para a produção de giberelina apresentaram fenótipo anão e esta mutação já foi revertida com a aplicação de ácido giberélico exógeno. A anatomia de plântulas cultivadas in vitro e ex vitro fornece informações que podem auxiliar na melhoria das técnicas de micropropagação e na sua seleção precoce. Objetivou-se verificar a diferenciação das estruturas anatômica entre plantas normais e variantes somaclonais. Para tal, utilizaram-se folhas de 'Prata-anã' cultivadas in vitro, em fase de aclimatização e no campo após a constatação da variação somaclonal. Secções paradérmicas foram realizadas para avaliação do complexo estomático. Secções transversais foram realizadas para medições da espessura das faces adaxial e abaxial da epiderme, medições dos parênquimas clorofilianos e da nervura central. Os resultados permitiram verificar diferenças estruturais das plantas ao longo das fases de cultivo. Foi possível também determinar marcadores anatômicos baseados no diâmetro da nervura central e na hipoderme inferior que permiti distinguir as plantas normais das variantes somaclonais.Micropropagation of Musa plants is applied on a wide scale in order to obtain plantlets with high sanitary quality. In vitro cultivation may lead to somaclonal variation that can cause harm the producers. Currently, differentiation of somaclonal variants is possible only in the field based on the exaggerated growth of the plants. Mutants for gibberellin production are dwarfed

  13. Two AAA family peroxins, PpPex1p and PpPex6p, interact with each other in an ATP-dependent manner and are associated with different subcellular membranous structures distinct from peroxisomes.

    Science.gov (United States)

    Faber, K N; Heyman, J A; Subramani, S

    1998-02-01

    Two peroxins of the AAA family, PpPex1p and PpPex6p, are required for peroxisome biogenesis in the yeast Pichia pastoris. Cells from the corresponding deletion strains (Pp delta pex1 and Pp delta pex6) contain only small vesicular remnants of peroxisomes, the bulk of peroxisomal matrix proteins is mislocalized to the cytosol, and these cells cannot grow in peroxisome-requiring media (J. A. Heyman, E. Monosov, and S. Subramani, J. Cell Biol. 127:1259-1273, 1994; A. P. Spong and S. Subramani, J. Cell Biol. 123:535-548, 1993). We demonstrate that PpPex1p and PpPex6p interact in an ATP-dependent manner. Genetically, the interaction was observed in a suppressor screen with a strain harboring a temperature-sensitive allele of PpPEX1 and in the yeast two-hybrid system. Biochemially, these proteins were coimmunoprecipitated with antibodies raised against either of the proteins, but only in the presence of ATP. The protein complex formed under these conditions was 320 to 400 kDa in size, consistent with the formation of a heterodimeric PpPex1p-PpPex6p complex. Subcellular fractionation revealed PpPex1p and PpPex6p to be predominantly associated with membranous subcellular structures distinct from peroxisomes. Based on their behavior in subcellular fractionation experiments including flotation gradients and on the fact that these structures are also present in a Pp delta pex3 strain in which no morphologically detectable peroxisomal remnants have been observed, we propose that these structures are small vesicles. The identification of vesicle-associated peroxins is novel and implies a role for these vesicles in peroxisome biogenesis. We discuss the possible role of the ATP-dependent interaction between PpPex1p and PpPex6p in regulating peroxisome biogenesis events. PMID:9447990

  14. GenBank blastx search result: AK288054 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK288054 J075152E14 DQ088099.1 DQ088099 Musa acuminata genotype AAA chloroplast ribulose-1,5-bis ... hosphate carboxylase/oxygenase small subunit (rbcS-Ma1 ) gene, complete cds; nuclear gene for chloroplast ...

  15. GenBank blastx search result: AK288517 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK288517 J090043B03 DQ088099.1 DQ088099 Musa acuminata genotype AAA chloroplast ribulose-1,5-bis ... hosphate carboxylase/oxygenase small subunit (rbcS-Ma1 ) gene, complete cds; nuclear gene for chloroplast ...

  16. Agrobacterium-mediated transformation of Musa spp.cv.Tianbao with cDNA encodina S6PDH(NADP-dependent sorbitol-6-phosphate dehydrogenase) from Prunus salicina var.cordata%根癌农杆菌介导的榇S6PDH基因转化香蕉研究

    Institute of Scientific and Technical Information of China (English)

    匡云波; 赖钟雄

    2012-01-01

    研究以香蕉栽培品种“天宝蕉”(Musa spp.cv.Tianbao)横切薄片(Thin cross-sections,TCSs)为材料,采用根癌农杆菌介导的方法,进行棒S6PDH基因转化香蕉的研究.结果表明,在横切薄片继代增殖培养基M4中添加5%~7%(V/V)的椰汁明显增强了香蕉芽苗的生长势;GUS基因瞬时表达检测表明,长势旺盛的香蕉芽苗(直径为7~8 mm)适宜作为香蕉遗传转化的受体材料,横切薄片厚度以2mm左右为佳;采用两步法进行抗性芽的筛选得到37个抗性芽苗,生根移栽后获得31株成活苗;目的基因S6PDH和报告基因GUS的PCR检测表明其中4株是转基因植株.该研究为将蔷薇科山梨醇代谢途径引入香蕉以提高其耐渗透胁迫的能力奠定了重要的基础.%Musa spp. cv. Tianbao was transformed with cDNA encoding S6PDH(NADP-dependent sorbitol-6-phosphate dehydrogenase) isolated from Prunus sa/icina var. cordata by an Agrobacterium-mediated thin cross-sections (TCSs) transformation system. The condition of the buds was effectively improved when the TCSs were transferred onto the medium M4 adding 5%-7%(V/V) coconut water. And the highest GUS transient expression occurred while 2 mm thin TCSs from the healthy and strong buds were used as the recepted material. Total 37 putative transformants were selected via the two-step method and 31 putative transformants survived after transplanting. Finally, four transgenic lines were conformed by PCR analysis of S6PDH gene and GUS gene. Sorbitol synthesis pathway which was unique to the Rosaceae plants had been introduced into Musa spp.cv.Tianbao, laying the groundwork to increase its tolerance to environmental stress.

  17. 食管癌调强放疗计划中AAA算法与PBC算法的对比研究%A Comparison of Anisotropic Analytical Algorithm (AAA) and Pencil Beam Convolution (PBC) Algorithm for IMRT Treatment Planning of Esophageal Carcioma

    Institute of Scientific and Technical Information of China (English)

    谭丽娜; 石梅; 柴广金; 孙晓欢; 马奎; 肖锋

    2012-01-01

    目的:比较Varian治疗计划系统Eclipse中AAA算法和PBC算法在食管癌调强放疗中的剂量学差异.方法:选择22例中段食管癌患者,分别采用AAA算法与PBC算法设计两种调强计划,比较靶区的剂量分布,肺、脊髓和心脏等危及器官受照剂量的差异.结果:PGTV最大剂量、PTV平均剂量和左肺的平均剂量两种算法无显著性差异(P>0.05),PGTV和PTV最小剂量、PTV最大剂量、参考剂量所包靶区的体积(V95)和其他危及器官的受量,两种算法均有显著性差异(P<0.05).结论:与PBC算法相比,AAA算法对不均匀组织的修正更加精确,对于食管癌这种与肺相关的剂量计算采用AAA算法更准确一些.%Objective: The aim of the study was to investigate the difference of Anisotropic Analytical Algorithm (AAA) and Pencil Beam Convolution (PBC) Algorithm in the Eclipse planning system(Varian Medical Systems) for Esophageal Carcioma IMRT Treatment Planning. Methods: For 22 patients with esophageal carcioma, IMRT Treatment Plannings with AAA and PBC were preformed respectively. The difference of dose distribution to target and organs at risk (OAR) was compared. Re-sults: There was no significant difference in the maximum doses of the PGTV, the mean doses of the PTV and left lung (P>0. 05), while there was significant difference in the minimum doses of the PGTV and PTV, the maximum doses of the PTV, the percentage volume of the target receiving a dose greater than 95% of the prescription dose (V95), and the dose of other OAR (P<0.05). Conclusions: Compared with the PBC algorithm, AAA algorithm is more exactitude to correct the asymmetry tis-sue, so for the esophageal carcioma, AAA algorithm is more accurate.

  18. Efecto de la temperatura en la reacción de oxidación de almidón de plátano (Musa paradisiaca L.). Estimación de la energía de activación

    OpenAIRE

    M.M. Sánchez-Rivera; L.A. Bello-Pérez

    2008-01-01

    El almidón nativo de plátano (Musa paradisiaca L.) fue sometido a cinéticas de oxidación con hipoclorito de sodio (NaClO) al 2 % de cloro activo (p/v) a diferentes temperaturas (30 ºC, 40 ºC y 50 ºC) y dos pH (7.0 y 8.5). Las constantes de velocidad (k) y la energía de activación (Ea) se obtuvieron a partir de la concentración de hipoclorito ClO-, (expresado como cloro consumido) durante la oxidación del almidón. Se utilizaron ecuaciones de primer y segundo grado para determinar el orden de r...

  19. Caracterización física, morfológica y evaluación de las curvas de empastamiento de musáceas (Musa spp.

    Directory of Open Access Journals (Sweden)

    Javier Darío Hoyos-Leyva

    2012-07-01

    Full Text Available Se caracterizaron veinte variedades de musáceas de diferente composición genética: AB, BB, AAA, AAB, ABB, AAAA y AAAB, del Banco de Germoplasma existente en la hacienda Las Vegas, de Fedeplátano, Chinchiná (1360 m.s.n.m., 4° 58' N y 75° 20' O, Colombia. La caracterización incluyó propiedades físicas como peso, diámetro, longitud, materia seca y porcentaje de cáscara del fruto y propiedades funcionales como harinas y almidones. El análisis de componentes principales (ACP mostró que las variedades de plátano se diferencian por su mayor tamaño (peso, longitud y diámetro. M. B. Tani, ICAFHIA 110, Saba, y los plátanos del subgrupo Bluggoe tuvieron altos porcentajes de cáscara. Entre los demás clones no se observaron diferencian entre sí. Los plátanos de cocción del subgrupo Plantain presentaron el mayor contenido de materia seca. Bocadillo Chileno e híbridos postre (exceptuando FHIA-1, presentaron mayor contenido de fracción comestible frente a las demás variedades. La temperatura de inicio de gelatinización de las harinas en el RVA varió entre 66.58 °C para la variedad Bocadillo Chileno y 75.21 °C para Mbindi. La viscosidad máxima varió entre 441.57 y 1837.17 cP para las variedades Tafetán Rojo y Dwarf Cavendish, respectivamente. La facilidad de cocción varió entre 2.76 min en Bocadillo Chileno y 7.55 min en Gros Michel Guayabo. La temperatura de inicio de gelatinización de los almidones varió entre 65.58°C para Gros Michel Guayabo y 74.41°C en Tafetán Rojo. La variedad Yangambi Km3 presentó la viscosidad máxima a 483.24 cP e Indio a 1958.44cP. La variedad Mbindi presentó la mayor facilidad de cocción (1.91 min mientras que FHIA-1 presentó el mayor tiempo (9.49 min.

  20. Ángel, musa y duende

    OpenAIRE

    Ossa Martinez, Marco Antonio de la

    2014-01-01

    in duda alguna, Federico García Lorca es uno de los nombres más destacados en la historia de la literatura. Sin embargo, su relación con la música es una faceta sobre la que merece la pena detenerse: «Ante todo, soy músico», dijo en una entrevista realizada en 1933. No sólo fue un buen intérprete de piano, sino que conoció a los principales compositores de la España de su época. Además, fue un amante del flamenco y de la música tradicional y cabe destacar la grabación Canciones populares espa...

  1. Progress report on Musa and cassava

    International Nuclear Information System (INIS)

    The Plant Breeding Unit develops nuclear methods and related technology, such as in vitro culture techniques, molecular biology and carries out physiological studies on the genetic improvement of crop plants. The Unit transfers these technologies by providing training in plant breeding, mutagenic treatment service and technical advice. A number of activities were undertaken in relation to the CRP ''Improvement of basic food crops in African through plant breeding including the use of induced mutations''. 5 refs, 1 fig., 1 tab

  2. Proyecto de Innovación AAA_14_059. Cuestionarios de Evaluación sobre Diseño, Realización, Resultados y Satisfacción con las Prácticas de Laboratorio y Taller

    OpenAIRE

    2014-01-01

    EVALUACIÓN Y MEJORA DE LAS PRÁCTICAS DE TALLER Y LABORATORIO DE LAS ÁREAS DE CONOCIMIENTO DE CIENCIA DE LOS MATERIALES E INGENIERÍA METALÚRGICA Y DE QUÍMICA INORGÁNICA. Proyecto de Innovación Docente. Acción avalada: AAA_14_059 Una vez que los estudios de Grado están implantados y que se ha elegido un conjunto de prácticas para ilustrar los conceptos teóricos, transcurridos cuatro años se considera necesario evaluar lo hecho y plantear propuestas de revisión y mejora. La revisión se p...

  3. BANANO Y REVALUACIÓN EN EL DEPARTAMENTO DEL MAGDALENA,1997-2007

    OpenAIRE

    JOAQUÍN VILORIA DE LA HOZ

    2008-01-01

    El objetivo de la presente investigación es estudiar el comportamiento de la economíabananera del departamento del Magdalena durante la última década (1997-2007) yanalizar los efectos que ha tenido la revaluación del peso sobre este sector económico.En este período, la actividad bananera arrojó algunas cifras preocupantes y otrasesperanzadoras: de una parte, el área cosechada, la producción y el valor de real de lasexportaciones disminuyeron. Pero también, el precio implícito de la fruta en d...

  4. Extracción de taninos del banano verde de rechazo

    OpenAIRE

    Ángela María Velásquez Valderrama

    2005-01-01

    Introducción: Una de las aplicaciones más evidente de la práctica biotecnológica en la bioindustria es el proceso fermentativo con organismos como los hongos y las bacterias, los cuales a partir de un sustrato orgánico, obtienen la energía necesaria para su crecimiento y desarrollo. Durante el mecanismo cinético de crecimiento e inhibición celular, uso de sustrato y mantenimiento, se da simultáneamente la formación de productos metabólicos de desecho de gran importa...

  5. Phenylphenalenones Accumulate in Plant Tissues of Two Banana Cultivars in Response to Herbivory by the Banana Weevil and Banana Stem Weevil.

    Science.gov (United States)

    Hölscher, Dirk; Buerkert, Andreas; Schneider, Bernd

    2016-01-01

    Phenylphenalenone-type compounds accumulated in the tissues of two banana cultivars-Musa acuminata cv. "Grande Naine" (AAA) and Musa acuminata × balbisiana Colla cv. "Bluggoe" (ABB)-when these were fed on by the banana weevil (Cosmopolites sordidus (Germ.) (Coleoptera: Curculionidae)) and the banana stem weevil (Odoiporus longicollis (Oliver) (Coleoptera: Curculionidae)). The chemical constituents of the banana material were separated by means of chromatographic techniques and identified by NMR spectroscopy. One new compound, 2-methoxy-4-phenylphenalen-1-one, was found exclusively in the corm material of "Bluggoe" that had been fed on by the weevils. PMID:27571112

  6. 7种龙舌兰属植物提取物对两种炭疽病病菌抑菌活性初探%Antifungal activity of extracts from 7 species of Agave spp.plants against Colletotrichum gloeosporioides and Calletotrichum musae

    Institute of Scientific and Technical Information of China (English)

    2016-01-01

    采用菌丝生长速率法测定了7种龙舌兰属植物提取物对胶孢炭疽病病菌(Colletotrichum gloeosporioides)和香蕉炭疽病病菌(Calletotrichum musae)的抑菌活性.结果表明,在质量浓度10、5 mg/mL下处理72 h,7种龙舌兰属植物提取物对两种炭疽病病菌均有很强的抑茵活性.其中,H.11648、广西76416、有剌番麻和番麻对两种炭疽病病菌抑菌率均为100%,普通剑麻、粤西114和灰叶剑麻对两种炭疽病病菌抑菌率为77.78%~100%.毒力测定表明,有刺番麻对胶孢和香蕉炭瘟病病菌抑茵效果最显著,有效中浓度(EC5o)分别为0.027 7 mg/mL和0.048 2 mg/mL,其次是广西76416,有效中浓度(EC50)分别为0.093 4 mg/mL和0.240 9 mg/mL.

  7. Nematode resistance in bananas : screening results on some new Mycosphaerella resistant banana hybrids

    OpenAIRE

    Quénéhervé, Patrick; Salmon, F.; Topart, Patrick; Horry, J. P.

    2009-01-01

    Banana hybrids with resistance to Yellow Sigatoka and Black Leaf Streak disease were evaluated for resistance to the burrowing nematode Radopholus similis and to the lesion nematode Pratylenchus coVeae in a growth chamber at 24-28. Plants produced by tissue culture were acclimatised for 6 weeks prior to inoculation. Forty-five days after inoculation with nematodes, the root systems were processed and nematode numbers assessed. Two cultivars of Grande Naine (Musa AAA, Cavendish subgroup, ITC12...

  8. Wastes of banana ‘s lignocellulosic biomass: a sustainable and renewable source of biogas production

    OpenAIRE

    Kamdem, Irenée; Hiligsmann, Serge; Vanderghem, Caroline; Bilik, Igor; Paquot, Michel

    2013-01-01

    We highlight in this poster, the results of biogas production and biochemical analysis based on the anaerobic digestion of each type of the lignocellulosic waste from a banana cultivar (Williams Cavendish: triploid Musa AAA group). These wastes are usually abandoned in the plantation after the fruits have been harvested. There is great interest in obtaining energy from this generally neglected biomaterial, particularly in the contexts of global warming and sustainable development.

  9. Efecto de la densidad de inoculación en la formación y morfología de los embriones somáticos de plátano (Musa spp. AAAB, cv. híbrido FHIA-21

    Directory of Open Access Journals (Sweden)

    Leyanis García-Águila

    2010-10-01

    Full Text Available Título en inglés: Effect of inoculum density on formation and morphology of plantain somatic embryos (Musa spp. AAAB, cv. Hybrid ‘FHIA-21’ Resumen La embriogénesis somática constituye una interesante propuesta para la propagación masiva de plantas del cultivar híbrido FHIA-21 (Musa AAAB. Sin embargo, se caracteriza por ser un proceso asincrónico, dado por la presencia de embriones somáticos en diferentes etapas de desarrollo ontogénico. La reducción de la asincronía es un aspecto importante para la utilización de este sistema de regeneración en la propagación de plantas. Esta investigación se realizó con el objetivo de determinar el efecto de la densidad de inoculación sobre la formación y el desarrollo morfológico homogéneo de los embriones somáticos del cv. FHIA-21. Con este propósito se adicionaron 0,5; 1,0; 1,5 y 2,0 g de masa fresca (gMF de agregados celulares embriogénicos en Erlenmeyers (250 ml, que contenían 30 ml de medio de cultivo líquido. Las evaluaciones se realizaron a los 30 días de cultivo con la determinación del número y la longitud de los embriones somáticos (mm. Además de una descripción morfológica e histológica de estos. Los resultados mostraron el efecto regulatorio de la densidad de inoculación en la formación y el desarrollo morfológico de los embriones somáticos. La mayor formación de embriones con un desarrollo morfológico homogéneo se produjo en 1,5 gMF de densidad de inoculación. Esta proporcionó un 86,4% de embriones con una longitud de 0,21 a 4,0 mm, y el estudio de las secciones histológicas mostró evidencias histológicas de la etapa globular. La sincronización durante la formación y el desarrollo de los embriones somáticos del cv. FHIA-21 aumentó la eficiencia del proceso de embriogénesis somática. Palabras clave: histología; morfología; embriogénesis somática; sincronización. Abstract Somatic embryogenesis is an interesting proposal for mass propagation

  10. Careers Other Than Academia EPA/AAAS Graduate Fellowship Expo

    Science.gov (United States)

    NCER invited Emilie to speak to the EPA STAR graduate fellows about her transition from graduate school into a postdoctoral position with the EPA. Discussion of what led Emilie to seek a position with the EPA, how she conducted her job search, and what qualities she thinks gradu...

  11. 26 CFR 1.1368-2 - Accumulated adjustments account (AAA).

    Science.gov (United States)

    2010-04-01

    ... depletion over the basis of property subject to depletion unless the property is an oil or gas property the... of the shareholders' deductions for depletion for any oil or gas property held by the corporation... the adjusted basis of which exceeds its fair market value on the date of the distribution and...

  12. Dimensions of Usability: Cougaar, Aglets and Adaptive Agent Architecture (AAA)

    Energy Technology Data Exchange (ETDEWEB)

    Haack, Jereme N.; Cowell, Andrew J.; Gorton, Ian

    2004-06-20

    Research and development organizations are constantly evaluating new technologies in order to implement the next generation of advanced applications. At Pacific Northwest National Laboratory, agent technologies are perceived as an approach that can provide a competitive advantage in the construction of highly sophisticated software systems in a range of application areas. An important factor in selecting a successful agent architecture is the level of support it provides the developer in respect to developer support, examples of use, integration into current workflow and community support. Without such assistance, the developer must invest more effort into learning instead of applying the technology. Like many other applied research organizations, our staff are not dedicated to a single project and must acquire new skills as required, underlining the importance of being able to quickly become proficient. A project was instigated to evaluate three candidate agent toolkits across the dimensions of support they provide. This paper reports on the outcomes of this evaluation and provides insights into the agent technologies evaluated.

  13. Mechanism of Amyloidogenesis of a Bacterial AAA+ Chaperone.

    Science.gov (United States)

    Chan, Sze Wah Samuel; Yau, Jason; Ing, Christopher; Liu, Kaiyin; Farber, Patrick; Won, Amy; Bhandari, Vaibhav; Kara-Yacoubian, Nareg; Seraphim, Thiago V; Chakrabarti, Nilmadhab; Kay, Lewis E; Yip, Christopher M; Pomès, Régis; Sharpe, Simon; Houry, Walid A

    2016-07-01

    Amyloids are fibrillar protein superstructures that are commonly associated with diseases in humans and with physiological functions in various organisms. The precise mechanisms of amyloid formation remain to be elucidated. Surprisingly, we discovered that a bacterial Escherichia coli chaperone-like ATPase, regulatory ATPase variant A (RavA), and specifically the LARA domain in RavA, forms amyloids under acidic conditions at elevated temperatures. RavA is involved in modulating the proper assembly of membrane respiratory complexes. LARA contains an N-terminal loop region followed by a β-sandwich-like folded core. Several approaches, including nuclear magnetic resonance spectroscopy and molecular dynamics simulations, were used to determine the mechanism by which LARA switches to an amyloid state. These studies revealed that the folded core of LARA is amyloidogenic and is protected by its N-terminal loop. At low pH and high temperatures, the interaction of the N-terminal loop with the folded core is disrupted, leading to amyloid formation. PMID:27265850

  14. Nuclear Forensics: Report of the AAAS/APS Working Group

    Science.gov (United States)

    Tannenbaum, Benn

    2008-04-01

    This report was produced by a Working Group of the American Physical Society's Program on Public Affairs in conjunction with the American Association for the Advancement of Science Center for Science, Technology and Security Policy. The primary purpose of this report is to provide the Congress, U.S. government agencies and other institutions involved in nuclear forensics with a clear unclassified statement of the state of the art of nuclear forensics; an assessment of its potential for preventing and identifying unattributed nuclear attacks; and identification of the policies, resources and human talent to fulfill that potential. In the course of its work, the Working Group observed that nuclear forensics was an essential part of the overall nuclear attribution process, which aims at identifying the origin of unidentified nuclear weapon material and, in the event, an unidentified nuclear explosion. A credible nuclear attribution capability and in particular nuclear forensics capability could deter essential participants in the chain of actors needed to smuggle nuclear weapon material or carry out a nuclear terrorist act and could also encourage states to better secure such materials and weapons. The Working Group also noted that nuclear forensics result would take some time to obtain and that neither internal coordination, nor international arrangements, nor the state of qualified personnel and needed equipment were currently enough to minimize the time needed to reach reliable results in an emergency such as would be caused by a nuclear detonation or the intercept of a weapon-size quantity of material. The Working Group assesses international cooperation to be crucial for forensics to work, since the material would likely come from inadequately documented foreign sources. In addition, international participation, if properly managed, could enhance the credibility of the deterrent effect of attribution. Finally the Working Group notes that the U.S. forensics capability involved a number of agencies and other groups that would have to cooperate rapidly in an emergency and that suitable exercises to ensure such cooperation were needed.

  15. AAA Roadmap for Navigating Religion in Physical Education

    Science.gov (United States)

    Kahan, David

    2011-01-01

    The majority of Americans identify with a religious faith, and the connection between religious beliefs/behaviors and health behaviors make it important to understand teachers' role and influence in this regard. The purpose of this article is to inform readers regarding (1) religious demographics in the United States; (2) the relationship between…

  16. The 40th AAAS Gordon Conference on nuclear chemistry

    International Nuclear Information System (INIS)

    I am pleased to speak at the Fortieth Gordon Conference on Nuclear Chemistry. I served as Chairman of the first Gordon Conference on Nuclear Chemistry held June 23--27, 1952, at New Hampton, New Hampshire. In my remarks, during which I shall quote from my journal, I shall describe some of the background leading up to the first Gordon Conference on Nuclear Chemistry and my attendance at the first seven Gordon Conferences during the period 1952 through 1958. I shall also quote my description of my appearance as the featured speaker at the Silver Anniversary of the Gordon Research Conferences on December 27, 1956 held at the Commodore Hotel in New York City. I shall begin with reference to my participation in the predecessor to the Gordon Conferences, the Gibson Island Research Conferences 45 years ago, on Thursday, June 20, 1946, as a speaker. This was 15 years after the start of these conferences in 1931. Neil Gordon played a leading role in these conferences, which were named (in 1948) in his honor -- the Gordon Research Conferences -- soon after they were moved to Colby Junior College, New London, New Hampshire in 1947. W. George Parks became Director in 1947, Alexander Cruickshank became Assistant Director in 1947 and Director in 1968

  17. Antimicrobial Treatment to Impair Expansion of Abdominal Aortic Aneurysm (AAA)

    DEFF Research Database (Denmark)

    Bergqvist, David; Lindeman, Johannes H N; Lindholt, Jes Sanddal;

    2013-01-01

    Antimicrobial treatment to attenuate expansion of abdominal aortic aneurysm has been suggested, especially with the focus on Chlamydophila. In this systematic literature review only four randomized trials were identified. In two small studies there is an indication of an effect of roxithromycin. In...

  18. The 40th AAAS Gordon Conference on nuclear chemistry

    Energy Technology Data Exchange (ETDEWEB)

    Seaborg, G.T.

    1991-06-27

    I am pleased to speak at the Fortieth Gordon Conference on Nuclear Chemistry. I served as Chairman of the first Gordon Conference on Nuclear Chemistry held June 23--27, 1952, at New Hampton, New Hampshire. In my remarks, during which I shall quote from my journal, I shall describe some of the background leading up to the first Gordon Conference on Nuclear Chemistry and my attendance at the first seven Gordon Conferences during the period 1952 through 1958. I shall also quote my description of my appearance as the featured speaker at the Silver Anniversary of the Gordon Research Conferences on December 27, 1956 held at the Commodore Hotel in New York City. I shall begin with reference to my participation in the predecessor to the Gordon Conferences, the Gibson Island Research Conferences 45 years ago, on Thursday, June 20, 1946, as a speaker. This was 15 years after the start of these conferences in 1931. Neil Gordon played a leading role in these conferences, which were named (in 1948) in his honor -- the Gordon Research Conferences -- soon after they were moved to Colby Junior College, New London, New Hampshire in 1947. W. George Parks became Director in 1947, Alexander Cruickshank became Assistant Director in 1947 and Director in 1968.

  19. Analisis Kandungan Inulin pada Pisang Barangan (Musa acuminata Colla), Pisang Awak (Musa paradisiaca var Awak) dan pisang kepok (Musa acuminata balbisiana Colla)

    OpenAIRE

    Wahyuningsih, Defi

    2015-01-01

    Inulin is a substance of food that has a function as a prebiotic that is good for the development and activity of nonpathogenic bacteria in the digestive system. One source of inulin is bananas. Bananas are consumed by all people ranging from infants to adults. Inulin in bananas can help improve the health. One of which is to increase the body's immunity with his role as a prebiotic. This study aims to determine the content of inulin on banana barangan , banana awak , and banana kepok well...

  20. Improvement of plantain, Musa spp. using mutation breeding techniques

    International Nuclear Information System (INIS)

    Tissue culture regenerated plants of 'Apantu' and 'Asamienu' varieties of plantain were acutely irradiated using gamma rays at 0,30 and 40 Gy, rooted in soil, and transferred to a field for agronomic evaluation and selection. A proposal to select dwarf mutants is reported. (author). 4 refs, 1 tab

  1. Karyological Observations in Musa beccarii var. hottana (Musaceae)

    Czech Academy of Sciences Publication Activity Database

    Häkkinen, M.; Suchánková, Pavla; Doleželová, Marie; Hřibová, Eva; Doležel, Jaroslav

    2007-01-01

    Roč. 58, 2/3 (2007), s. 112-118. ISSN 1346-7565 R&D Projects: GA MŠk(CZ) LC06004; GA AV ČR IAA600380703 Institutional research plan: CEZ:AV0Z50380511 Source of funding: V - iné verejné zdroje ; V - iné verejné zdroje Keywords : Callimusa * chromosome number * flow cytometry Subject RIV: EB - Genetics ; Molecular Biology http://ci.nii.ac.jp/naid/110006533812/en/

  2. Molecular and Cytogenetic Characterization of Wild Musa Species

    Czech Academy of Sciences Publication Activity Database

    Čížková, Jana; Hřibová, Eva; Christelová, Pavla; Van den Houwe, I.; Häkkinen, M.; Roux, N.; Swennen, R.; Doležel, Jaroslav

    2015-01-01

    Roč. 10, č. 8 (2015), e0134096. E-ISSN 1932-6203 R&D Projects: GA MŠk(CZ) LG12021; GA MŠk(CZ) LO1204 Institutional support: RVO:61389030 Keywords : NUCLEAR-DNA CONTENT * GENETIC DIVERSITY * RIBOSOMAL DNA Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.234, year: 2014

  3. Carmen Dauset Moreno, primera musa del cine estadounidense

    OpenAIRE

    Mora Contreras, Francisco Javier

    2014-01-01

    Este artículo se propone analizar la importancia de la bailarina española Carmen Dauset Moreno para la historia del cine. Conocida en el mundo del vaudeville como Carmencita, fue la primera mujer que apareció en dos breves películas para kinetoscopio realizadas en el estudio de Edison en marzo de 1894. En este sentido, el artículo trata de contextualizar la aparición de la artista española en estas películas y plantea cuestiones sobre el significado de las mismas en el desarrollo de las fases...

  4. ALLOCATIVE EFFICIENCY AND RESOURCE USE IN BANANA ( Musa sapientum A ND PLANTAIN ( Musa paradisiaca PRODUCTION ENTERPRISES IN BAYELSA STATE , NIGERIA

    Directory of Open Access Journals (Sweden)

    Kainga Prince Ebiowei

    2013-11-01

    Full Text Available Present study was conducted to determine the allocative efficiency and resource use of banana and plantain production enterprises in Bayelsa State of Nigeria. Three - stage sampling technique was used for drawing the samples and getting data. Total sample size was 180 farm households. The data were analyzed by using mean and production function models. Allocative efficiency and resource use results indicates farmers incapabil ity in efficient use and allocation of banana and plantain suckers, family labor and hired labor. In relation to total revenue, the elasticity of the plantain suckers showed that, an increase in it by one unit will lead to an increase in total revenue. Results from the allocative efficiency index, objective of profit maximization and equimarginal principle, indicated that banana suckers were efficiently allocated, while plantain suckers were inefficiently allocated; family and hired labor were inefficiently allocated in both banana and plantain enterprises. While plantain suckers were under - utilized, family and hired labor were both over - utilized in both enterprises, suggesting that farmers were yet to achieve absolute degree of allocative efficiency in thes e resources. However, they could profitably reduce the quantity of family and hired labor and increase plantain suckers that they employ. Thus, allocative efficiency and resource use can be enhanced by the provision of credit/ loan, good rural roads, impro ved varieties of suckers and farming technologies, extension services and subsidized farm inputs and equipment among others by Government, Non - Governmental Organizations (NGOs and institutions alike through genuine political will

  5. Crescimento de mudas micropropagadas da bananeira cv. Nanicão, em diferentes substratos e fontes de fertilizante = Growth of micropropagated cavendish banana seedlings in different substrates and fertilizer sources

    OpenAIRE

    Edson Shigueaki Nomura; Juliana Domingues Lima; Valéria Augusta Garcia; Domingos Sávio Rodrigues

    2008-01-01

    Com objetivo de avaliar o efeito de diferentes substratos combinados a fontes de fertilizantes no crescimento de mudas de bananeira micropropagadas cv. Nanicão (Musa spp. AAA), foi instalado um experimento em blocos casualizados, no esquema fatorial 5 x 3,com quatro repetições. Os substratos utilizados foram: S1 - Terra de subsolo + casca de arroz carbonizada + Rendmax Floreira®; S2 - Terra de subsolo + casca de arroz carbonizada + Organifol®; S3 - Terra de subsolo + casca de arroz carbonizad...

  6. 5个香蕉穿孔线虫种群对香蕉的致病性测定%Determination of Pathogenicity of 5 Populations of Radopholus similis to 3 Banana Cultivars

    Institute of Scientific and Technical Information of China (English)

    符美英; 吴凤芝; 卜小莉; 王会芳; 陈绵才

    2011-01-01

    Radopholus similis is the most serious pest of banana. The pathogenicity of 5 populations of R. Similis to seedlings of 3 banana cultivars was tested by pot-inoculation in greenhouse. The results showed that all of the 5 populations of R. Similis were highly pathogenic to Musa AAA Giant Cavendish cv. Baxi. RSI population of R. Similis showed weak pathogenicity to Musa ABB Pisang Awak and the other four populations showed no pathogenicity to Musa ABB Pisang Awak. Musa acuminata cv. Mas was not the host of these 5 R. Similis populations. Musa AAA Giant Cavendish cv. Baxi plants parasitized with R. Similis showed a reduced growth with yellowing leaves and browning spot in roots.%香蕉穿孔线虫对香蕉生产是一种毁灭性的危害.为了明确5个香蕉穿孔线虫种群对香蕉的致病性,在温室中采用盆栽接种的方法,分别测定了5个香蕉穿孔线虫花卉种群对巴西蕉、皇帝蕉和粉蕉的寄生性.结果表明,5个香蕉穿孔线虫种群对巴西蕉都有强致病性;RS1种群对粉蕉存在弱致病性,其他4个种群对粉蕉均没有致病性;皇帝蕉不是这5个香蕉穿孔线虫种群的寄主植物.香蕉穿孔线虫寄生巴西蕉后,造成香蕉植株明显矮小、长势弱,严重时地上部叶片出现萎蔫黄化,根部形成褐色病斑甚至坏死变黑腐烂等症状,对巴西蕉危害显著.

  7. Caracterización molecular de 15 aislamientos de Beauveria bassiana asociados con Cosmopolites y Metamasius en plátano y banano en tres regiones de Colombia

    Directory of Open Access Journals (Sweden)

    Muñoz Flórez Jaime Eduardo

    2008-09-01

    Full Text Available Se colectaron picudos de Cosmopolites y Metamasius en municipios del Valle del Cauca, Caldas y Quindío. Se obtuvieron cultivos monospóricos con diluciones de 10-10 y 10-11. Los aislamientos fueron almacenados a -80°C con glicerol al 10% y el ADN a –20°C. Los marcadores moleculares RAM generaron 82 fragmentos de los cuales 67% fueron polimórficos con una heterocigocidad de 0.24, que indica diversidad media a alta. A un índice de similitud 0.84 se formaron 5 grupos: uno con 11 aislamientos y 4 con un solo aislamiento. En el gran grupo se detectó un duplicado y se encontró diversidad del hongo en los sitios muestreados. No se encontró relación entre aislamientos sobre Cosmopolites y Metamasius o zona geográfica en la formación de grupos genéticos.

  8. Formas de carbono orgánico en suelos con diferentes usos en el departamento del Magdalena (Colombia

    Directory of Open Access Journals (Sweden)

    José Rafael Vásquez-Polo

    2011-12-01

    Full Text Available Las fracciones de materia orgánica del suelo (MOS lábiles y humificadas pueden ser afectadas por las prácticas de uso y manejo; sin embargo el impacto de estos cambios no se ha evaluado en suelos y ambientes tropicales. El presente estudio tuvo como objetivo evaluar los contenidos y algunas formas de carbono orgánico del suelo (COS en cinco zonas de clima cálido tropical (0 - 1110 m.s.n.m. del departamento del Magdalena (Colombia y el efecto que sobre ellas han tenido las prácticas asociadas a suelos cultivados con café (Coffea arabica, banano (Musa sp., palma africana (Elaeis guineensis y sábila (Aloe vera, comparados con suelos de bosques naturales. No se encontraron diferencias (P < 0.05 tanto entre zonas como entre usos del suelo, se presentaron valores medios a bajos de MOS en las zonas de estudio y contenidos de carbono total (Ct mayores en suelos de bosques que en suelos cultivados, así los suelos de bosques presentan una acumulación media de Ct de 42.4 mg/ha a 20 cm, frente a 33.8 mg/ha en los suelos cultivados, esto equivale a una pérdida media de Ct del 23% por efecto del manejo de los cultivos. En relación con el carbono extraíble con pirofosfato sódico altamente relacionado con las fracciones humificadas de la MOS (Cp se observaron valores muy bajos en los suelos cultivados y casi nulos en suelos de bosques; sin embargo estos últimos presentan mayor contenido de formas de carbono no-oxidables o estables (Cnox determinado por diferencia entre Ct - carbono oxidable (Cox. En el suelo cultivado con banano, el Ct corresponde en su totalidad a formas de Cox; mientras que en el suelo cultivado con palma africana las formas estables Cnox representaron 83% del carbono total.

  9. Formas de carbono orgánico en suelos con diferentes usos en el departamento del Magdalena (Colombia

    Directory of Open Access Journals (Sweden)

    Menjivar Flores Juan Carlos

    2011-12-01

    Full Text Available Las fracciones de materia orgánica del suelo (MOS lábiles y humificadas pueden ser afectadas por las prácticas de uso y manejo; sin embargo el impacto de estos cambios no se ha evaluado en suelos y ambientes tropicales. El presente estudio tuvo como objetivo evaluar los contenidos y algunas formas de carbono orgánico del suelo (COS en cinco zonas de clima cálido tropical (0 - 1110 m.s.n.m. del departamento del Magdalena (Colombia y el efecto que sobre ellas han tenido las prácticas asociadas a suelos cultivados con café (Coffea arabica, banano (Musa sp., palma africana (Elaeis guineensis y sábila (Aloe vera, comparados con suelos de bosques naturales. No se encontraron diferencias (P < 0.05 tanto entre zonas como entre usos del suelo, se presentaron valores medios a bajos de MOS en las zonas de estudio y contenidos de carbono total (Ct mayores en suelos de bosques que en suelos cultivados, así los suelos de bosques presentan una acumulación media de Ct de 42.4 mg/ha a 20 cm, frente a 33.8 mg/ha en los suelos cultivados, esto equivale a una pérdida media de Ct del 23% por efecto del manejo de los cultivos. En relación con el carbono extraíble con pirofosfato sódico altamente relacionado con las fracciones humificadas de la MOS (Cp se observaron valores muy bajos en los suelos cultivados y casi nulos en suelos de bosques; sin embargo estos últimos presentan mayor contenido de formas de carbono no-oxidables o estables (Cnox determinado por diferencia entre Ct - carbono oxidable (Cox. En el suelo cultivado con banano, el Ct corresponde en su totalidad a formas de Cox; mientras que en el suelo cultivado con palma africana las formas estables Cnox representaron 83% del carbono total.

  10. Diagnosis and perioperative management of ruptured AAA mimicking symptomatic groin hernia

    OpenAIRE

    Klein, Holger Jan; Becker, Daniel; Rancic, Zoran

    2016-01-01

    INTRODUCTION: Ruptured abdominal aortic aneurysm (RAAA) can infrequently present as symptomatic groin hernia. This misleading form of presentation often leads to erroneous preoperative management resulting in poor survival. CASE PRESENTATION: Two patients with RAAA mimicking symptomatic groin hernia underwent different preoperative managements pointing out the importance of the principles of hypotensive haemostasis in the scope of this emergency scenario. CONCLUSION: Computed Tomography...

  11. Diagnosis and perioperative management of ruptured AAA mimicking symptomatic groin hernia

    OpenAIRE

    Holger Jan Klein; Daniel Becker; Zoran Rancic

    2016-01-01

    Introduction: Ruptured abdominal aortic aneurysm (RAAA) can infrequently present as symptomatic groin hernia. This misleading form of presentation often leads to erroneous preoperative management resulting in poor survival. Case presentation: Two patients with RAAA mimicking symptomatic groin hernia underwent different preoperative managements pointing out the importance of the principles of hypotensive haemostasis in the scope of this emergency scenario. Conclusion: Computed Tomography...

  12. Detectability of motions in AAA with ECG-gated CTA : A quantitative study

    NARCIS (Netherlands)

    Klein, Almar; Oostveen, Luuk J.; Greuter, Marcel J. W.; Hoogeveen, Yvonne; Kool, Leo J. Schultze; Slump, Cornelis H.; Renema, W. Klaas Jan

    2009-01-01

    Purpose: ECG-gated CT enables the visualization of motions caused by the beating of the heart. Although ECG gating is frequently used in cardiac CT imaging, this technique is also very promising for evaluating vessel wall motion of the aortic artery and the motions of (stent grafts inside) abdominal

  13. Introductory Chemistry Needs A Revolution. ACS and IUPAC and AAAS and ICUC Should Lead It

    Science.gov (United States)

    Hawkes, Stephen J.

    2005-01-01

    The standard curriculum for introductory chemistry does not reflect the needs of students, is enforced by custom and untenured teachers who deviate from it endanger prospects for tenure. Hence, revision of the introductory curriculum should involve research into actual needs and interests of students and should reflect what modern workers conceive…

  14. Ethical Issues and the Life Sciences. Test Edition. AAAS Study Guides on Contemporary Problems.

    Science.gov (United States)

    Kieffer, George H.

    This is one of several study guides on contemporary problems produced by the American Association for the Advancement of Science with support of the National Science Foundation. This study guide on Ethical Issues and the Life Sciences includes the following sections: (1) Introduction; (2) The Search for an Ethic; (3) Biomedical Issues including…

  15. An Instructors Guide to Water Pollution. Test Edition. AAAS Study Guides on Contemporary Problems, No. 5.

    Science.gov (United States)

    Kidd, David E.

    This is one of several study guides on contemporary problems produced by the American Association for the Advancement of Science with support of the National Science Foundation. This study guide on water pollution includes the following units: (1) Overview of World Pollution; (2) History, Definition, Criteria; (3) Ecosystem Theory; (4) Biological…

  16. Science, Science Signaling, and Science Translational Medicine – AAAS Special Collection on Cancer Research, March 2011

    Directory of Open Access Journals (Sweden)

    Forsythe, Katherine H.

    2011-10-01

    Full Text Available The National Cancer Act, signed in 1971, aimed to eliminate cancer deaths through a massive increase in research funding. The American Association for the Advancement of Science, the publisher of Science, Science Signaling, and Science Translational Medicine, observed the 40th anniversary of the Cancer Act in 2011, with special research articles and features, found in all three journals, on the state of cancer research 40 years later. This collection of articles explores both breakthroughs and the challenges in cancer research over the last four decades, and lets us know what we might expect in the future.

  17. Distinct static and dynamic interactions control ATPase-peptidase communication in a AAA+ protease

    OpenAIRE

    Martin, Andreas; Baker, Tania A.; Sauer, Robert T.

    2007-01-01

    In the ClpXP proteolytic machine, ClpX uses the energy of ATP hydrolysis to unfold protein substrates and translocate them through a central pore and into the degradation chamber of ClpP. Here, we demonstrate a bipartite system of ClpX-ClpP interactions that serves multiple functional roles. High-affinity contacts between six loops near the periphery of the hexameric ClpX ring and a ClpP ring establish correct positioning and increase degradation activity but are insensitive to nucleotide sta...

  18. Diagnosis and perioperative management of ruptured AAA mimicking symptomatic groin hernia

    Directory of Open Access Journals (Sweden)

    Holger Jan Klein

    2016-01-01

    Conclusion: Computed Tomography Angiography (CTA remains the recommended diagnostic tool—for both safe diagnosis of the ruptured aneurysm and precise preoperative planning. Endovascular aortic repair of the RAAA—if feasible—is the treatment of choice. This rare form of RAAA manifestation should call physicians attention—especially in patients with known abdominal aortic aneurysms in their preceding medical history.

  19. Regeneration and multiplication capacity of local banana (Musa spp.) cultivars using callus and shoot tip cultures

    International Nuclear Information System (INIS)

    Conventional clonal propagation of banana cultivars using suckers is very slow and limited. In Malaysia, areas for banana plantation are increasing and there is an urgent demand for a quick source of planting materials. In addition, there is interest in improving and developing local banana cultivars for commercial production. In vitro culture techniques can provide an effective method for rapid micropropagation of new banana plants. Plantlet regeneration of P. Intan was achieved via callus tissues derived from the cell suspension cultures. The planting materials for P. Nangka and P. Tanduk were difficult to obtain because of poor sucker formation. However, this study showed that the multiplication rate of plantlets of these cultivars is high and potentially useful in producing high and uniform planting materials

  20. Species of beetles (Coleoptera; Scarabaeidae associated to banana (Musa spp. in Ceballos, Ciego de Avila, Cuba

    Directory of Open Access Journals (Sweden)

    Maria Luisa Sisne Luis

    2016-03-01

    Full Text Available A white light trap was placed in bananas plantations, according to Sisne, 2009 and MINAG, 1985, in the Citric enterprise of Ciego de Ávila during the period between May and July of 2010 with the objective of determining the composition of genus and species of the order Coleoptera family Scarabaeidae associated to the agroecosystem. The species Cyclocephala cubana Chapin, Phyllophaga puberula Duval, and Phyllophaga patruelis Chev. are associated to bananas crops in these areas.

  1. Identification List of Ensete and Musa (Musaceae) in SE Asia and West Malesia

    OpenAIRE

    HOTTA, Mitsuru; ホッタ, ミツル

    1989-01-01

    This identification list refers to specimens of Herbarium of Kyoto University (KYO), Herbarium Bogoriense (BO), Singapore Botanic Gardens' Herbarium (SING), Rijkshebarium (L), Herbarium of Forest Department, Kuching(SAR) and Herbarium of Forest Department, Sandakan (SAN).

  2. Identification List of Ensete and Musa (Musaceae) in SE Asia and West Malesia

    OpenAIRE

    HOTTA, Mitsuru; ホッタ, ミツル

    1989-01-01

    This identification list refers to specimens of Herbarium of Kyoto University (KYO), Herbarium Bogoriense (BO), Singapore Botanic Gardens' Herbarium (SING), Rijkshebarium (L), Herbarium of Forest Department, Kuching (SAR) and Herbarium of Forest Department, Sandakan (SAN).

  3. Genomics of banana and plantain (Musa spp.), major staple crops in the tropics

    Czech Academy of Sciences Publication Activity Database

    Roux, N.; Baurens, F.C.; Doležel, Jaroslav; Hřibová, Eva; Heslop-Harrison, P.; Town, C.; Sasaki, T.; Matsumoto, T.; Aert, R.; Remy, S.; Souza, M.; Lagoda, P.

    New York : Springer, 2008 - (Moore, P.; Ming, R.), s. 83-111 ISBN 978-0-387-71218-5 R&D Projects: GA AV ČR IAA600380703 Institutional research plan: CEZ:AV0Z50380511 Keywords : Banana and plantain * genomics * BAC libraries Subject RIV: EB - Genetics ; Molecular Biology

  4. COMPONENTES QUÍMICOS DA FARINHA DE BANANA (Musa sp.) OBTIDA POR MEIO DE SECAGEM NATURAL

    OpenAIRE

    João Miguel de Moraes Neto; Luiza Eugênia da M. Rocha Cirne; Juarez Paz Pedroza; Mozaniel Gomes da Silva

    1998-01-01

    RESUMO Objetivando-se obter farinha de baixo custo e seu possível aproveitamento como alimento adicional para as crianças das creches e escolas da zona rural, banana verde e madura foram submetidas a secagem natural, durante 18 horas. As bananas maduras sofreram secagem adicional em estufa a 60ºC, durante 24 horas; posteriormente, avaliou-se a composição química da farinha por meio das análises de cinzas, carboidratos totais, açúcares redutores e proteínas. A análise estatística revelou que, ...

  5. INVESTIGATION OF IMMUNOSTIMULATORY BEHAVIOUR OF MUSA ACUMINATE PEEL EXTRACT IN CLARIAS BATRACHUS

    Directory of Open Access Journals (Sweden)

    Prit Benny, Geetha Viswanathan, Smitha Thomas, Aruna Nair

    2010-08-01

    Full Text Available Antibiotics provide a useful means of helping to control many bacterial diseases but there are many problems associated with the development of antibiotic resistance and recurrent outbreaks necessity further, costly treatments. Immunostimulants especially when administered through the diet have been potentially playing an important role in aquaculture. Banana fruit peels are generally considered as waste, but recently antifungal, antimicrobial and antioxidant properties are explored in the peel. The banana is nourishing, doesn’t contain fat, and can be eaten at every hour of the day because of its digestive properties. The banana contains magnesium, selenium, iron, a lot of vitamins and is recommended for salt free diet because of its low content in sodium chloride. Generally, the peel is being thrown once the pulp is eaten. These peels are eaten by animals or sometimes degraded by nature. Our aim is to extract few vital substances. The protein content of the peel was estimated qualitatively and quantitatively. The extract was injected into Clarias batrachus and its immune cells were counted. The result shows the wasted peel has both lower and higher molecular weights and has an immunological response as it showed an increase in the immune cells and there were very less cellular distortions when histopathological observations was done.

  6. DETERMINATION OF THE ISOSTERIC HEAT TO PLANTAIN PULP (musa paradisiaca BY SORPTION ISOTHERMS

    Directory of Open Access Journals (Sweden)

    HÉCTOR CIRO

    2008-01-01

    Full Text Available Las isotermas de sorción para ser usadas en la simulación y diseño de procesos de secado y almacenamiento de alimentos deben ser representadas por modelos válidos dentro de las condiciones encontradas en la práctica industrial. Usando el modelo de Chung-Pfost y ajustado por desorción el calor isostérico neto y totalpara pulpa de plátano fueron determinados. El calor neto isotérico neto decreció con el contenido de humedad del producto variando desde 1670 kJ/kg (5%.d.b a 215 kJ/kg (26%.d.b donde esta variación fue representada por el modelo potencial y polinomial.

  7. Endophytic bacterial diversity in banana 'Prata Anã' (Musa spp. roots

    Directory of Open Access Journals (Sweden)

    Suzane A. Souza

    2013-01-01

    Full Text Available The genetic diversity of endophytic bacteria in banana 'Prata Anã' roots was characterized. Two hundred and one endophytic bacteria were isolated, 151 of which were classified as Gram-positive and 50 as Gram-negative. No hypersensitivity response was observed in any of the isolates. The rep-PCR technique generated different molecular profiles for each primer set (REP, ERIC and BOX. Fifty readable loci were obtained and all of the fragments were polymorphic. Amplified ribosomal DNA restriction analysis (ARDRA of the isolates based on cleavage with four restriction enzymes yielded 45 polymorphic bands and no monomorphic bands. PCR amplified the nifH gene in 24 isolates. 16S rDNA sequencing of the 201 bacterial isolates yielded 102 high-quality sequences. Sequence analyses revealed that the isolates were distributed among ten bacterial genera (Agrobacterium, Aneurinibacillus, Bacillus, Enterobacter, Klebsiella, Lysinibacillus, Micrococcus, Paenibacillus, Rhizobium and Sporolactobacillus and included 15 species. The greatest number of isolates belonged to the genus Bacillus. The bacteria identified in this study may be involved in promoting growth, phosphate solubilization, biological control and nitrogen fixation in bananas.

  8. Modelo de rendimiento potencial del cultivo de plátano (musa aab simmonds)

    OpenAIRE

    Chaves, Bernardo

    2010-01-01

    El plátano es un producto básico en la alimentación para más de 400 millones de habitantes de las regiones tropicales y subtropicales del mundo. Los modelos del desarrollo de cultivos se han convertido en una herramienta de mucha utilidad para investigadores que estudian procesos fisiológicos básicos, y agricultores que los usan en la toma de decisiones para el manejo del cultivo. Con el objetivo de desarrollar un modelo de producción potencial de plátano, se usaron los datos de dos experimen...

  9. Feeding of banana (Musa spp.) plantation wastes for fast pyrolysis process

    Science.gov (United States)

    Abdullah, Nurhayati; Sulaiman, Fauziah; Taib, Rahmad Mohd

    2013-05-01

    Using the pyrolysis process, agricultural residue such as banana waste can be converted into bio-char, bio-oil, and gases. The fast pyrolysis process of banana wastes on the available 150g/h rig requires particle size reduction. The particle size of less than 150μm constitutes 50% and particles in the 150-250μm ranges make up 28% of the distribution of particle size of banana leaves. The particle size of less than 150μm makes up 52% and particles in the 150-250μm ranges constitute 28% of the distribution of particle size for banana pseudo-stem. A new gravity chute feeder is also designed for this fast pyrolysis system. A series of feeding tests were conducted using this new feeder. The advantages and limitations will be presented. A comparison with the previously designed feeder will be discussed.

  10. Rheological properties of emulsions stabilized by green banana (Musa cavendishii pulp fitted by power law model

    Directory of Open Access Journals (Sweden)

    Dayane Rosalyn Izidoro

    2009-12-01

    Full Text Available In this work, the rheological behaviour of emulsions (mayonnaises stabilized by green banana pulp using the response surface methodology was studied. In addition, the emulsions stability was investigated. Five formulations were developed, according to design for constrained surfaces and mixtures, with the proportion, respectively: water/soy oil/green banana pulp: F1 (0.10/0.20/0.70, F2 (0.20/0.20/0.60, F3 (0.10/0.25/0.65, F4 (0.20/0.25/0.55 and F5 (0.15/0.225/0.625 .Emulsions rheological properties were performed with a rotational Haake Rheostress 600 rheometer and a cone and plate geometry sensor (60-mm diameter, 2º cone angle, using a gap distance of 1mm. The emulsions showed pseudoplastic behaviour and were adequately described by the Power Law model. The rheological responses were influenced by the difference in green banana pulp proportions and also by the temperatures (10 and 25ºC. The formulations with high pulp content (F1 and F3 presented higher shear stress and apparent viscosity. Response surface methodology, described by the quadratic model,showed that the consistency coefficient (K increased with the interaction between green banana pulp and soy oil concentration and the water fraction contributed to the flow behaviour index increase for all emulsions samples. Analysis of variance showed that the second-order model had not significant lack-of-fit and a significant F-value, indicating that quadratic model fitted well into the experimental data. The emulsions that presented better stability were the formulations F4 (0.20/0.25/0.55 and F5 (0.15/0.225/0.625.No presente trabalho, foi estudado o comportamento reológico de emulsões adicionadas de polpa de banana verde utilizando a metodologia de superfície de resposta e também foram investigadas a estabilidade das emulsões. Foram desenvolvidas cinco formulações, de acordo com o delineamento para superfícies limitadas e misturas, com as proporções respectivamente: água/óleo de soja/polpa de banana verde: F1 (0,10/0,20/0,70, F2 (0,20/0,20/0,60, F3 (0,10/0,25/0,65, F4 (0,20/0,25/0,55 e F5 (0,15/0,225/0,625. As propriedades reológicas foram realizadas em reômetro Haake Rheostress 600, com sensor de geometria cone e placa (60-mm diâmetro, 2º ângulo de cone, usando uma distância de abertura de 1mm. As emulsões mostraram comportamento pseudoplástico e foram descritas adequadamente pelo modelo Lei da Potência. O comportamento reológico foi influenciado pelas diferentes proporções de polpa de banana verde e também pelas temperaturas (10 e 25ºC. As formulações com alto teor de polpa (F1 e F3 apresentaram maior tensão de cisalhamento e viscosidade aparente. Relativo à metodologia de superfície de resposta, descrita pelo modelo quadrático, pôde-se observar que o coeficiente de consistência aumentou com a interação entre a polpa de banana verde e a concentração de óleo de soja e a água contribuiu com o aumento do índice de comportamento para todas as amostras de emulsões. A Análise de Variância mostrou que o modelo de segundo ordem não apresentou falta-de-ajuste com valor F significativo, indicando que o modelo quadrático representou bem os dados experimentais. As emulsões que apresentaram melhor estabilidade foram as formulações F4 (0,20/0,25/0,55 e F5 (0,15/0,225/0,625.

  11. Banana (Musa sp. var. elakki bale) flower and pseudostem: dietary fiber and associated antioxidant capacity.

    Science.gov (United States)

    Bhaskar, Jamuna J; S, Mahadevamma; Chilkunda, Nandini D; Salimath, Paramahans V

    2012-01-11

    Banana flower (BF) and pseudostem (PS) are byproducts of banana cultivation and are known to have health beneficial effects. The main objective of this study was to evaluate the dietary fiber composition and antioxidant effect of BF and PS. In the present study, BF and PS were found to be rich in dietary fiber (65.6 ± 1.32 and 28.8 ± 0.98%, respectively). Dietary fiber fractions were extracted and characterized in terms of sugar profile, and antioxidant activities were determined. BF and PS fractions were rich in sugars and showed wide diversity with respect to the nature of the sugars. Hemicellulose A fraction of BF showed high amounts of total polyphenols and total antioxidants, which were 121.8 ± 1.9 and 39.03 ± 0.118 μg/mg extract, respectively. HPLC analysis showed the presence of phenolic acids in hemicellulose A and B fractions of BF. These results indicate that BF and PS are rich sources of dietary fiber associated with polyphenols, which could promote health beneficial effects. PMID:22122826

  12. Modelling change in color and firmness of baby banana (musa acuminata aa) in modified atmosphere packaging

    OpenAIRE

    Castellanos Espinosa, Diego; Algecira Enciso, Néstor

    2012-01-01

    To determine the change in the ripening stage and quality through associated variables such as firmness and peel color is a useful tool for predicting the behavior and involvement of the product stored at different changing conditions. The change in O2 and CO2 concentration, pulp firmness and peel color were measured in a test of modified atmosphere packaging for baby banana to develop a mathematical model to represent the change in firmness and color as a function of temperature, mixture of ...

  13. Repetitive part of the banana (Musa acuminata) genome investigated by low-depth 454 sequencing

    Czech Academy of Sciences Publication Activity Database

    Hřibová, Eva; Neumann, Pavel; Matsumoto, T.; Roux, N.; Macas, Jiří; Doležel, Jaroslav

    2010-01-01

    Roč. 10, č. 204 (2010), s. 1-10. ISSN 1471-2229 R&D Projects: GA AV ČR KJB500380901; GA AV ČR IAA600380703; GA MŠk(CZ) LC06004 Institutional research plan: CEZ:AV0Z50380511; CEZ:AV0Z50510513 Keywords : IN-SITU HYBRIDIZATION * RIBOSOMAL- RNA GENES * DNA-SEQUENCES Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 4.085, year: 2010

  14. Effect of gamma-irradiation on post-harvest ripening of plantain fruit (Musa paradisiaca) cultivars

    International Nuclear Information System (INIS)

    Three plantain cultivars, Agbagba, Obino L'ewai and Cardaba, harvested at two stages of maturity, were irradiated at 0.1-1.0 kGy prior to storage under tropical ambient conditions. Postirradiation storage behaviour showed that plantain fruits at full three-quarter grade were more responsive to irradiation treatment than at full grade maturity, while Cardaba, a type of cooking banana, showed the least response. Irrespective of varietal differences, irradiation to 0.5 kGy or higher caused severe tissue damage, softening and skin discolouration; however, gamma-irradiation at optimum dose range of 0.15-0.30 kGy effectively delayed ripening for 10-12 days in Agbagba and Obino L'ewai, respectively, without affecting respiratory pattern or fruit quality

  15. Antioxidant effect of unripe plantain (Musa paradisiacae on oxidative stress in alloxan-induced diabetic rabbits

    Directory of Open Access Journals (Sweden)

    Eleazu C.O

    2012-01-01

    Full Text Available Background: Diabetes mellitus results in complications that could be contributed by elevated levels of free radicals and oxidative stress and increased risk of cardiovascular disease. Aim: We investigated the effect of the antioxidant activity of unripe plantain on oxidative stress markers in alloxan induced diabetic rabbits. Materials and Method: Rats were randomized into three groups; control, diabetic, and unripe plantain-treated diabetic rats. The proximate, phytochemical and antioxidant activity of the unripe plantain were determined. The glutathionine, malonaldehyde, catalase and glucose levels of the animals were also determined using standard techniques. Result: The diabetic rabbits placed on unripe plantain diet had a decrease in their blood glucose (- 24.25±8.08% and malonaldehyde levels with a corresponding increase in their weights (23.63±1.20%, glutathione and catalase levels after 3weeks of dieting compared with the control (p<0.05. Glutathione correlated negatively with malonaldehyde and glucose (r = -0.77 and -0.877, but positively with catalase and body weight (r = 0.60 and 0.70. Malonaldehyde correlated negatively with catalase and body weight (r = - 0.44 and -0.72 but positively with glucose (r = 0.86. Catalase correlated negatively with glucose(-0.69 but positively with body weight (1. The extract had a high scavenging activity on DPPH radical (78.57±0.12% comparable to that of standard vitamin C (83.20±0.00% in addition to a high total antioxidant activity (62±0.20%. The flour contained low amounts of protein, lipid, tannin, saponin, alkaloids, flavonoids and energy value but significant quantities of crude fibre, ash and carbohydrates. Conclusion: These findings suggest that raised blood glucose level in diabetics could deplete cells of their antioxidant status with resultant increase in oxidative stress. Finally, unripe plantain intake may exert a free radical scavenging activity by ameliorating the altered antioxidant status.

  16. Extraction of Cellulose from Kepok Banana Peel (Musa parasidiaca L. for Adsorption Procion Dye

    Directory of Open Access Journals (Sweden)

    Poedji Loekitowati Hariani

    2016-05-01

    cellulose. The morphology of cellulose more homogenous than kepok banana peel powder. It was observed that the optimum adsorption of Procion dye by cellulose was on the initial concentration of 30 mg/L, pH solution of 5 and contact time within 30 minutes. The obtained result that cellulose has removal percentage to adsorp Procion dye more higher than kepok banana peel powder. The adsorption equilibrium showed the Langmuir isotherm was described well for adsorption process (R2 = 0.991 than Freundlich isotherm (R2 = 0.922.

  17. Antioxidant effcacy of unripe banana (Musa acuminata Colla peel extracts in sunflower oil during accelerated storage

    Directory of Open Access Journals (Sweden)

    Stella Sye Chee Ling

    2015-12-01

    Full Text Available Background. Sunflower oil is prone to oxidation during storage time, leading to production of toxic com- pounds that might affect human health. Synthetic antioxidants are used to prevent lipid oxidation. Spreading interest in the replacement of synthetic food antioxidants by natural ones has fostered research on fruit and vegetables for new antioxidants. Material and methods. In this study, the efficacy of unripe banana peel extracts (100, 200 and 300 ppm  in stabilizing sunflower oil was tested under accelerated storage (65°C for a period of 24 days. BHA and α-tocopherol served as comparative standards besides the control. Established parameters such as peroxide value (PV, iodine value (IV, p-anisidine value (p-AnV, total oxidation value (TOTOX, thiobarbituric acid reactive substances (TBARS and free fatty acid (FFA content were used to assess the extent of oil deterioration. Results. After 24 days storage at 65°C, sunflower oil containing 200 and 300 ppm extract of unripe banana peel showed significantly lower PV and TOTOX compared to BHA and α-tocopherol. TBARS, p-AnV and FFA values of sunflower oil containing 200 and 300 ppm of unripe banana peel extract exhibited comparable inhibitory effects with BHA. Unripe banana peel extract at 200 and 300 ppm demonstrated inhibitory effect against both primary and secondary oxidation up to 24 days under accelerated storage conditions. Conclusions. Unripe banana peel extract may be used as a potential source of natural antioxidants in the ap- plication of food industry to suppress lipid oxidation.

  18. Pyrolytic oil of banana (Musa spp.) pseudo-stem via fast process

    Science.gov (United States)

    Abdullah, Nurhayati; Sulaiman, Fauziah; Taib, Rahmad Mohd; Miskam, Muhamad Azman

    2015-04-01

    This study was an attempt to produce bio-oil from banana pseudo-stem, a waste of banana cultivation, using fast pyrolysis technology. The compositions were determined and the thermal degradation behaviour of the raw material was analyzed using Perkin-Elmer Simultaneous Thermal Analyzer.(STA) 6000. A 300 g/h fluidized bed bench scale fast pyrolysis unit, assembled with double screw feeders and cyclones, operating at atmospheric pressure, was used to obtain the pyrolysis liquid. The study involves the impact of the following key variables; the reactor temperature in the range of 450-650°C, and the residence time in the range of 1.00-3.00s. The particle size was set at 224-400µm. The properties of the liquid product were analyzed for calorific heating value, pH value, conductivity, water and char content. The basic functional groups of the compositions were also determined using FTIR. The properties of the liquid product were compared with other wood derived bio-oil. The pyrolysis liquids derived from banana pseudo-stem were found to be in an aqueous phase.

  19. Pyrolytic oil of banana (Musa spp.) pseudo-stem via fast process

    Energy Technology Data Exchange (ETDEWEB)

    Abdullah, Nurhayati; Sulaiman, Fauziah; Taib, Rahmad Mohd [School of Physics, Universiti Sains Malaysia, 11800 USM Pulau Pinang (Malaysia); Miskam, Muhamad Azman [Science and Engineering Research Centre, Universiti Sains Malaysia, 14300 Nibong Tebal, Pulau Pinang (Malaysia)

    2015-04-24

    This study was an attempt to produce bio-oil from banana pseudo-stem, a waste of banana cultivation, using fast pyrolysis technology. The compositions were determined and the thermal degradation behaviour of the raw material was analyzed using Perkin-Elmer Simultaneous Thermal Analyzer (STA) 6000. A 300 g/h fluidized bed bench scale fast pyrolysis unit, assembled with double screw feeders and cyclones, operating at atmospheric pressure, was used to obtain the pyrolysis liquid. The study involves the impact of the following key variables; the reactor temperature in the range of 450–650 °C, and the residence time in the range of 1.00–3.00 s. The particle size was set at 224-400 µm. The properties of the liquid product were analyzed for calorific heating value, pH value, conductivity, water and char content. The basic functional groups of the compositions were also determined using FTIR. The properties of the liquid product were compared with other wood derived bio-oil. The pyrolysis liquids derived from banana pseudo-stem were found to be in an aqueous phase.

  20. Properties of a Mg2+ independent isocitrate lyase from gamma irradiated preclimacteric banana (Musa cavendishii)

    International Nuclear Information System (INIS)

    Isocitrate lyase (EC 4.1, 3.1) was purified seventy fold from gamma irradiated banana pulp tissue acetone powder. It showed an optimum pH of 6.0, and the Ksub(m) value for DL-isocitrate was 0.8 mM. Among the various metabolic inhibitors, oxaloacetate was found to be the most potent and its inhibition was competitive. The enzyme activity was not dependent on externally added Mg2+. The Mg2+ content of the purified enzyme was 10-12 ng/mg protein. A method for the detection of the two multiple forms of isocitrate lyase presented in this preparation was developed using 2,4-dinitrophenylhydrazine as detecting agent for glyoxylate formed during the isocitrate lyase reaction. (author)

  1. The banana (Musa acuminata) genome and the evolution of monocotyledonous plants

    Czech Academy of Sciences Publication Activity Database

    D´Hont, A.; Denoeud, F.; Aury, J. M.; Baurens, F. Ch.; Carreel, F.; Garsmeur, O.; Noel, B.; Bocs, S.; Droc, G.; Rouard, M.; Da Silva, C.; Jabbari, K.; Cardi, C.; Poulain, J.; Souquet, M.; Labadie, K.; Jourda, C.; Lengellé, J.; Rodier-Goud, M.; Alberti, A.; Bernard, M.; Correa, M.; Ayyampalayam, S.; Mckain, M. R.; Leebens-Mack, J.; Burgess, D.; Freeling, M.; Mbéguié-A-Mbéguié, D.; Chabannes, M.; Wicker, T.; Panaud, O.; Barbosa, J.; Hřibová, Eva; Heslop-Harrison, P.; Habas, R.; Rivallan, R.; Francois, P.; Poiron, C.; Kilian, A.; Burthia, D.; Jenny, Ch.; Bakry, F.; Brown, S.; Guignon, V.; Kema, G.; Dita, M.; Waalwijk, C.; Joseph, S.; Dievart, A.; Jaillon, O.; Leclercq, J.; Argout, X.; Lyons, E.; Almeida, A.; Jeridi, M.; Doležel, Jaroslav; Roux, N.; Risterucci, A. M.; Weissenbach, J.; Ruiz, M.; Glaszmann, J. Ch.; Quétier, F.; Yahiaoui, N.; Wincker, P.

    2012-01-01

    Roč. 488, č. 7410 (2012), s. 213-219. ISSN 0028-0836 Institutional research plan: CEZ:AV0Z50380511 Keywords : IN-SITU HYBRIDIZATION * SEQUENCE COUNT DATA * DNA-SEQUENCES Subject RIV: ED - Physiology Impact factor: 38.597, year: 2012

  2. Musa Qasimli, USSR-Turkey: From Normalization of Relations Till New Cold War (1960-1979)

    OpenAIRE

    ,

    2013-01-01

    However, the Cold War was reanimated at the end of the 1970s. Tension at the international and West-East competition level prevailed over the normalization trend. Suspicions continued to trouble relations. Factors that prevented the de- velopment of cooperation remained while clandestine conflicts between the two states continued. The Soviet invasion of Afghanistan and the deployment of U.S. Pershing II missiles in Western Europe worsened the situation

  3. COBERTURAS VIVAS DE LEGUMINOSAS EN EL PLÁTANO (Musa sp. FHIA 03

    Directory of Open Access Journals (Sweden)

    I. R. Gutiérrez

    2002-01-01

    Full Text Available La necesidad de disminuir el consumo de agrotóxicos en el manejo del cultivo del plátano posibilita la búsqueda de alternativas viables en la esfera agroecológica, razones por las que en la Estación Experimental de la Universidad de Ciego de Avila, entre 1996 y 1998, se desarrolló un experimento para la determinación del efecto de coberturas vivas de leguminosas en una plantación de plátano FHIA-03, que se estableció en un suelo Ferralítico Rojo (Eutric ferralsol, evaluándose los efectos que coberturas vivas de las leguminosas Centrosema plumieri, Teramnus labialis y Stylosanthes guianensis produjeron en el agroecosistema, usando como unidad experimental parcelas de ocho plantas evaluables del cultivo principal (78 m2, en un diseño en franjas en el transcurso de dos años de establecimiento de la cobertura, obteniéndose una reducción significativa de las especies de malezas características del área y competitivas del cultivo, un comportamiento favorable de los indicadores fenológicos, tendencias positivas en cuanto a elementos que pueden definir la calidad del suelo así como de reducción de algunas de las plagas que ocasionan los mayores daños en el plátano. Por último, se apreció la elevación de los rendimientos del cultivo principal y un efecto económico positivo en el manejo del agroecosistema, especialmente con el uso de Teramnus labialis como cobertura.

  4. Wie wirken "effektive Mikroorganismen" auf EM-Bokashi in der Bananenproduktion (Musa ssp.)?

    OpenAIRE

    Formowitz, B.; Elango, F.; Okumoto, S.; Buerkert, A.; Müller, T

    2005-01-01

    In Costa Rica, ‘effective microorganisms’ (EM) are used to produce organic fertiliser in the form of Bokashi. This study aimed at investigating the effects of EM addition on the decomposition of banana residues during Bokashi production in comparison to different non-EM control variants (Bokashi produced with: W= water, M= molasses as an EM additive, EMst= sterilized EM). Furthermore, the effects of the above mentioned Bokashi variants on the growth of young banana plants and their effects on...

  5. A duplex PCR assay for the detection of Ralstonia solanacearum phylotype II strains in Musa spp.

    Science.gov (United States)

    Cellier, Gilles; Moreau, Aurélie; Chabirand, Aude; Hostachy, Bruno; Ailloud, Florent; Prior, Philippe

    2015-01-01

    Banana wilt outbreaks that are attributable to Moko disease-causing strains of the pathogen Ralstonia solanacearum (Rs) remain a social and economic burden for both multinational corporations and subsistence farmers. All known Moko strains belong to the phylotype II lineage, which has been previously recognized for its broad genetic basis. Moko strains are paraphyletic and are distributed among seven related but distinct phylogenetic clusters (sequevars) that are potentially major threats to Musaceae, Solanaceae, and ornamental crops in many countries. Although clustered within the Moko IIB-4 sequevar, strains of the epidemiologically variant IIB-4NPB do not cause wilt on Cavendish or plantain bananas; instead, they establish a latent infection in the vascular tissues of plantains and demonstrate an expanded host range and high aggressiveness toward Solanaceae and Cucurbitaceae. Although most molecular diagnostic methods focus on strains that wilt Solanaceae (particularly potato), no relevant protocol has been described that universally detects strains of the Musaceae-infecting Rs phylotype II. Thus, a duplex PCR assay targeting Moko and IIB-4NPB variant strains was developed, and its performance was assessed using an extensive collection of 111 strains representing the known diversity of Rs Moko-related strains and IIB-4NPB variant strains along with certain related strains and families. The proposed diagnostic protocol demonstrated both high accuracy (inclusivity and exclusivity) and high repeatability, detected targets on either pure culture or spiked plant extracts. Although they did not belong to the Moko clusters described at the time of the study, recently discovered banana-infecting strains from Brazil were also detected. According to our comprehensive evaluation, this duplex PCR assay appears suitable for both research and diagnostic laboratories and provides reliable detection of phylotype II Rs strains that infect Musaceae. PMID:25811378

  6. Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA)

    Czech Academy of Sciences Publication Activity Database

    Pillay, M.; Ogundiwin, E.; Tenkouano, A.; Doležel, Jaroslav

    2006-01-01

    Roč. 5, - (2006), s. 1224-1232. ISSN 1684-5315 R&D Projects: GA MŠk(CZ) LC06004 Institutional research plan: CEZ:AV0Z50380511 Keywords : Banana * plantain * genomes Subject RIV: EB - Genetics ; Molecular Biology

  7. Pesan Al Quran dalam Kerjasama Musa dengan Syu'ayb tentang Pengelolaan Bisnis

    Directory of Open Access Journals (Sweden)

    Alimin Mesra

    2015-10-01

    Full Text Available Prophet Moses and Prophet Syu'ayb companion ultimately led them to work together. This case reveals a number of values that should be developed and applied in life related to business management. This story reveals a relation between employees and owners of capitla, recruitment patterns, appreciation of performance, proffesionalism, and some related matters. The cooperation between them can be considered as a symbolic learning delivered by the Quran, especially in business development. This story also shows that a partnership should be based on a clear contract that shows such related materials, working period, the amount of wages, security, and others related to their right and obligation.DOI: 10.15408/aiq.v4i1.2080

  8. Salomé, a musa do fim-do-século

    Directory of Open Access Journals (Sweden)

    Álvaro Cardoso Gomes

    2009-04-01

    Full Text Available  This article on Mallarmé’s poem “Salomé” analyses the character as a fin de siècle topusbecause she destroys males with her cold eroticism. In this sense, Salomé is seen more as a symbol, as a representation of a period, rather than as an historical or mythical figure

  9. Phenolic profiling in the pulp and peel of nine plantain cultivars (Musa sp.).

    Science.gov (United States)

    Passo Tsamo, Claudine Valérie; Herent, Marie-France; Tomekpe, Kodjo; Happi Emaga, Thomas; Quetin-Leclercq, Joëlle; Rogez, Hervé; Larondelle, Yvan; Andre, Christelle

    2015-01-15

    The present study investigated the phenolic profiles of the pulp and peel of nine plantain cultivars and compared them to those of two dessert bananas of commercial interest (Grand Nain and Gros Michel), alongside a newly created hybrid, resistant to black sigatoka disease (F568). Identification and quantification of phenolic compounds were performed by means of HPLC-ESI-HR-MS and HPLC-DAD. Hydroxycinnamic acids, particularly ferulic acid-hexoside with 4.4-85.1 μg/g of dry weight, dominated in the plantain pulp and showed a large diversity among cultivars. Flavonol glycosides were predominant in plantain peels, rutin (242.2-618.7 μg/g of dry weight) being the most abundant. A principal component analysis on the whole data revealed that the phenolic profiles of the hybrid, the dessert bananas and the pure plantains differed from each other. Plantain pulps and peels appeared as good sources of phenolics, which could be involved in the health benefits associated with their current applications. PMID:25148979

  10. Molecular Characterization of Banana streak virus Isolate from Musa Acuminata in China

    Institute of Scientific and Technical Information of China (English)

    Jun Zhuang; Jian-hua Wang; Xin Zhang; Zhi-xinLiu

    2011-01-01

    Banana streak virus (BSV),a member of genus Badnavirus,is a causal agent of banana streak disease throughout the world.The genetic diversity of BSVs from different regions of banana plantations has previously been investigated,but there are relatively few reports of the genetic characteristic of episomal (non-integrated)BSV genomes isolated from China.Here,the complete genome,a total of 7722bp (GenBank accession number DQ092436),of an isolate of Banana streak virus (BSV) on cultivar Cavendish (BSAcYNV) in Yunnan,China was determined.The genome organises in the typical manner of badnaviruses.The intergenic region of genomic DNA contains a large stem-loop,which may contribute to the ribosome shift into the following open reading frames (ORFs).The coding region of BSAcYNV consists of three overlapping ORFs,ORF 1 with a non-AUG start eodon and ORF2 encoding two small proteins are individually involved in viral movement and ORF3 encodes a polyprotein.Besides the complete genome,a defective genome lacking the whole RNA leader region and a majority of ORF1 and which encompasses 6525bp was also isolated and sequenced from this BSV DNA reservoir in infected banana plants.Sequence analyses showed that BSAcYNV has closest similarity in terms of genome organization and the coding assignments with an BSV isolate from Vientam (BSAcVNV).The corresponding coding regions shared identities of 88% and ~95% at nucleotide and amino acid levels,respectively.Phylogenetic analysis also indicated BSAcYNV shared the closest geographical evolutionary relationship to BSAcVNV among sequenced banana streak badnaviruses.

  11. Effectiveness of three micropropagation techniques to dissociate cytochimeras in Musa spp

    Czech Academy of Sciences Publication Activity Database

    Roux, N.; Doležel, Jaroslav; Swennen, R.; Zapata-Arias, F.

    2001-01-01

    Roč. 66, - (2001), s. 189-197. ISSN 0167-6857 R&D Projects: GA ČR GV521/96/K117 Institutional research plan: CEZ:AV0Z5038910 Keywords : chimera * flow cytometry * in vitro tissue culture Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 0.631, year: 2001

  12. In vivo interaction between ciprofloxacin hydrochloride and the pulp of unripe plantain (Musa paradisiaca).

    Science.gov (United States)

    Sv, Nwafor; Co, Esimone; Ca, Amadi; Cs, Nworu

    2003-01-01

    The absorption of quinolone antibiotics is seriously impaired by polyvalent cations due to the formation of unabsorbable complexes. M. paradisiaca Linn. (Musaceae), an important staple food in most parts of the world, has been demonstrated to contain many polyvalent cations. The aim of the work was to study the influence of concurrent administration of M. paradisiaca and ciprofloxacin HCI on the pharmacokinetic profiles of ciprofloxacin. The study was carried out in two phases in five healthy male rabbits. Phase one involved oral administration of ciprofloxacin (40 mg/kg) to rabbits, withdrawing blood from the marginal ear vein at 1, 2, 4, and 24 h intervals and checking the serum ciprofloxacin concentration. After a one-week drug "wash-out" period, the second phase started with concurrent oral administration of M. paradisiaca (800 mg/kg) and ciprofloxacin (40 mg/kg). Blood was again withdrawn and analyzed for serum ciprofloxacin content. Antimicrobial activity of the serum was also assessed and expressed as reciprocal serum inhibitory titer. Co-administration of both agents resulted in significant (Phour. The following pharmacokinetic parameters were also decreased: area under the curve (81.53%), peak serum concentration (94.37%), elimination rate constant (42.35%); while increase in half-life (81.08%) and clearance rate (69.64%) were noted. Antimicrobial study showed that the antimicrobial potency against E. coli was also decreased by such concurrent administration. The pharmacokinetic parameters and antimicrobial activities of ciprofloxacin were significantly decreased when it was given concurrently with pulp of unripe plantain. Complex formation between the drug and the polyvalent cations present in plantain, leading to decrease in absorption and hence bioavailability, may be responsible for the observed antagonistic interactions. PMID:14743965

  13. Benzothiadiazole-Mediated Induced Resistance to Colletotrichum musae and Delayed Ripening of Harvested Banana Fruit.

    Science.gov (United States)

    Zhu, Xiaoyang; Lin, Huanzhang; Si, Zhenwei; Xia, Yihua; Chen, Weixin; Li, Xueping

    2016-02-24

    Benzothiadiazole (BTH) works as a plant activator. The effects of different BTH treatments and fungicides SPORGON on fruit ripening and disease incidence were investigated. The results showed that BTH treatment significantly delayed fruit ripening, maintained fruit firmness, color, and good fruit quality, and dramatically reduced the incidence of disease. BTH effectively inhibited the invasion and development of pathogenic bacteria and controlled the occurrence of disease. BTH treatment enhanced the activities of defense-related enzymes, including chitinase, phenylalanine ammonia-lyase, peroxidase, and polyphenol oxidase, increased the content of hydrogen peroxide and total antioxidant capacity, and reduced malondialdehyde content. Cellular structure analysis after inoculation confirmed that BTH treatment effectively maintained the cell structural integrity. SPORGON did not provide benefits for delaying fruit ripening or for the resistance system, while it can control the disease only during the earlier stage and not at later stages. PMID:26871966

  14. PLÁTANO MACHO (Musa aab, CÁRDENAS, TABASCO, MÉXICO

    Directory of Open Access Journals (Sweden)

    Eder Ramos-Hern\\u00E1ndez

    2011-01-01

    Full Text Available Este experimento se realizó en Cárdenas, Tabasco, México en un suelo con textura franca, pH moderadamente ácido, contenido de materia orgánica y nitrógeno total bajo, cultivado con plátano macho, con el objetivo de determinar el control de arvenses con A. pintoi en una plantación de plátano macho. Se utilizó un arreglo factoria l alojado en un diseño de bloques completos al azar, con sombreado y tip o de cobertura vegetal asocia da al plátano como factores, con tres repe ticiones (octubre 2008-agosto 2009. Tanto el factor sombreado como el factor cobertura tuvie ron dos niveles de porcentaje: 45 (s= ± 11,1 y 50 (s= ± 13,2% de sombra, para suelos con arvenses y, con A. pintoi, respectivamente. Se utilizaron los índices de Shannon-Wie ver (H´ y similitud de Sörensen (S para determinar la riqueza de especie s. A los once meses después de establecido el experimento los valores de H´ no mostrarondiferencias significativas entre los tratamientos, S= 0,8 entre los dos niveles de sombra. Las especie s arvenses dominantes fueron: T. triangulare, S. podophylum, P. paniculatum y C. diffusa. Entre los tratamie ntos probados, el de suelo con arvenses a nivel de 50% de sombra fue el que presentó (p=0,05 mayor producción MS (375,3 ± 83,3 g/m2. La MS producida por A. pintoi fue mayor (p=0,05 con 45% (s= ± 11,1 de sombra (198 g/m2 que la producida a 50% (s= ± 13,2 sombra (150,4 g/m2. Se encontró que el nivel de efectividad de A. pintoi para controlar arvenses fue de 52,3% (con 45 ± 11,1% de sombra y 70,5% (con 50 ± 13,2% sombra.

  15. Banana Ovate Family Protein MaOFP1 and MADS-Box Protein MuMADS1 Antagonistically Regulated Banana Fruit Ripening

    OpenAIRE

    Juhua Liu; Jing Zhang; Wei Hu; Hongxia Miao; Jianbin Zhang; Caihong Jia; Zhuo Wang; Biyu Xu; Zhiqiang Jin

    2015-01-01

    The ovate family protein named MaOFP1 was identified in banana (Musa acuminata L.AAA) fruit by a yeast two-hybrid (Y2H) method using the banana MADS-box gene MuMADS1 as bait and a 2 day postharvest (DPH) banana fruit cDNA library as prey. The interaction between MuMADS1 and MaOFP1 was further confirmed by Y2H and Bimolecular Fluorescence Complementation (BiFC) methods, which showed that the MuMADS1 K domain interacted with MaOFP1. Real-time quantitative PCR evaluation of MuMADS1 and MaOFP1 ex...

  16. Avances de la fruticultura en México

    Directory of Open Access Journals (Sweden)

    Angel Villegas Monter

    2011-10-01

    Full Text Available Las estadísticas oficiales informaron que en 2008 se cultivaron 264 944 ha con valor de la producción de $ 14 741 millones de pesos, en 20 frutales de clima templado; mientras que, en 35 tropicales y subtropicales fue de 1 822748 ha con valor de $ 43 463 millones de pesos. De los 55 frutales, sólo naranjo (Citrus sinensis, mango (Mangifera indica, Aguacate (Persea americana, limón mexicano (C. aurantifolia, banano (Musa acuminata, lima persa (C. latifolia y manzano (Malus domestica se cultivan más de 50 000 ha, lo que explica porque es limitada la oferta de frutas en las grandes ciudades (Distrito Federal, Monterrey, Guadalajara, Puebla, Veracruz, entre otras. Considerando que el 79,27% de la superficie plantada con frutales tropicales y subtropicales es de temporal, las densidades de plantación son bajas, la propagación de plantas no se hace en viveros certificados (sólo cítricos, los rendimientos son bajos en comparación con otros países productores. Por otra parte, debido a la falta de técnicos capacitados, en los últimos 28 años la demanda de fruta se ha solucionado incrementando la superficie plantada, pero los rendimientos han disminuido. La presencia de nuevas enfermedades; Huanglongbing, Meleira, Sunblotch, plantean nuevos retos y también posibilidades si utilizamos los avances tecnológicos. La fruticultura es una alternativa viable pero deben utilizar altas densidades, árboles de porte bajo, plantas de origen genético conocido y calidad fitosanitaria probada que permitan tener mayores rendimientos y con ello competitividad.

  17. Formas de carbono orgánico en suelos con diferentes usos en el departamento del Magdalena (Colombia Some forms of organic carbon in soil with different uses in the Department of Magdalena (Colombia

    Directory of Open Access Journals (Sweden)

    José Rafael Vásquez-Polo

    2011-12-01

    Full Text Available Las fracciones de materia orgánica del suelo (MOS lábiles y humificadas pueden ser afectadas por las prácticas de uso y manejo; sin embargo el impacto de estos cambios no se ha evaluado en suelos y ambientes tropicales. El presente estudio tuvo como objetivo evaluar los contenidos y algunas formas de carbono orgánico del suelo (COS en cinco zonas de clima cálido tropical (0 - 1110 m.s.n.m. del departamento del Magdalena (Colombia y el efecto que sobre ellas han tenido las prácticas asociadas a suelos cultivados con café (Coffea arabica, banano (Musa sp., palma africana (Elaeis guineensis y sábila (Aloe vera, comparados con suelos de bosques naturales. No se encontraron diferencias (P Fractions of soil organic matter (SOM labile and humified, can be affected by use and management practices, but the impact of these changes has not been evaluated in soils of tropical environments. The present study investigated the contents and some forms of soil organic carbon (SOC in five warm tropical climate zones of the Department of Magdalena (Colombia, and the effect of the cropping practices on these forms of organic carbon in cultivated soils, associated with Coffee (Coffea arabica, Banana (Musa sp., African palm (Elaeis guineensis, Aloe (Aloe vera compared to natural forest soils. Significant differences (P < 0.05 were not found between zones as much use soil as. Low average values of SOM in the study areas and higher contents of total carbon in forest soils than in cultivated soils were reported. Forest soils had an average carbon accumulation total of 42.4 mg/ha at 20 cm, compared to 33.8 mg/ha in the cultivated soils, this equates to an average loss of 23% total C by the effect of crop management in these soils, compared to C humified (C extracted with sodium pyrophosphate, values are observed very low in cultivated soils and almost zero in forest soils, but forest soils had a higher number of stable forms of C (Cnox. In the soil cultivated

  18. A contig-based strategy for the genome-wide discovery of microRNAs without complete genome resources.

    Directory of Open Access Journals (Sweden)

    Jun-Zhi Wen

    Full Text Available MicroRNAs (miRNAs are important regulators of many cellular processes and exist in a wide range of eukaryotes. High-throughput sequencing is a mainstream method of miRNA identification through which it is possible to obtain the complete small RNA profile of an organism. Currently, most approaches to miRNA identification rely on a reference genome for the prediction of hairpin structures. However, many species of economic and phylogenetic importance are non-model organisms without complete genome sequences, and this limits miRNA discovery. Here, to overcome this limitation, we have developed a contig-based miRNA identification strategy. We applied this method to a triploid species of edible banana (GCTCV-119, Musa spp. AAA group and identified 180 pre-miRNAs and 314 mature miRNAs, which is three times more than those were predicted by the available dataset-based methods (represented by EST+GSS. Based on the recently published miRNA data set of Musa acuminate, the recall rate and precision of our strategy are estimated to be 70.6% and 92.2%, respectively, significantly better than those of EST+GSS-based strategy (10.2% and 50.0%, respectively. Our novel, efficient and cost-effective strategy facilitates the study of the functional and evolutionary role of miRNAs, as well as miRNA-based molecular breeding, in non-model species of economic or evolutionary interest.

  19. CHARACTERIZATION OF MALAYSIAN WILD BANANAS BASED ON ANTHOCYANINS

    OpenAIRE

    MUHAMMAD ASIF JAVED; MAK CHAI; ROFINA YASMIN OTHMAN

    2001-01-01

    The male buds of 16 Musa species (Musaceae) populations were investigated by HPLC for the occurrence of anthocyanins. The investigation was based on the presence of 6 anthocyanins. The 16 Musa samples could be classified into three distinct species i.e. Musa acuminata, Musa violascens and Musa balbisiana. Musa acuminata could be divided into two subspecies : malaccensis (lowland) and tmncata (highland) according to their constituents and content of major anthocyanins. No variation was ob...

  20. The Role of the N-Domain in the ATPase Activity of the Mammalian AAA ATPase p97/VCP*

    OpenAIRE

    Niwa, Hajime; Ewens, Caroline A.; Tsang, Chun; Yeung, Heidi O.; Zhang, Xiaodong; Freemont, Paul S.

    2012-01-01

    p97/valosin-containing protein (VCP) is a type II ATPase associated with various cellular activities that forms a homohexamer with each protomer containing an N-terminal domain (N-domain); two ATPase domains, D1 and D2; and a disordered C-terminal region. Little is known about the role of the N-domain or the C-terminal region in the p97 ATPase cycle. In the p97-associated human disease inclusion body myopathy associated with Paget disease of bone and frontotemporal dementia, the majority of m...

  1. Current technology for the treatment of infection following abdominal aortic aneurysm (AAA) fixation by endovascular repair (EVAR).

    Science.gov (United States)

    Capoccia, L; Mestres, G; Riambau, V

    2014-06-01

    In recent years, in parallel with the increase of endovascular aortic repair (EVAR) procedures performances, a rise of late open surgical removal of EVAR implants has been observed, due to non-endovascularly correctable graft complications. Among them endograft infection is a rare but devastating occurrence, accounting for an incidence ranging from 0.2% to 0.7% in major series, and almost 1% of all causes of endograft explantations. However, a real estimation of the incidence of the problem respect to the number of EVAR implantations is difficult to obtain. Time to infection is usually defined as the period between EVAR and presentation of symptoms that leads to the infection diagnosis. It can be extremely variable, depending on bacterial virulence and host conditions. The diagnosis of an endograft infection is usually based on a combination of clinical symptoms, imaging studies and microbial cultures whenever possible. If computed tomography (CT) scan is employed in almost 100% of infection diagnosis, a combination of fluorodeoxyglucose-positron emission tomography (FDG-PET) and CT scan is nowadays used with increasing frequency in order to rise the likelihood of detecting a graft infection, since even cultures of blood or samples collected from the infected field can sometimes be negative. Complete graft excision seems the best approach whenever a surgical reconstruction could be attempted. In situ reconstruction can be performed by the interposition of an autologous vein, a cryopreserved allograft or a rifampin-soaked Dacron graft. The so-called conventional treatment contemplates the re-establishment of vascularization through extranatomical routes, thus preserving the new graft material from possible contamination by the surgical field just cleaned. When severe comorbid conditions did not allow graft excision, a conservative treatment should be taken into account. It is mainly based on broad-spectrum or culture-specific antibiotic therapy combined, whenever possible, with percutaneous drainage of the infectious cavity or aneurismal sac followed by irrigation with saline and antibiotic solutions. New techniques of percutaneous drainage under CT scan guidance can allow expedite collection of fluid material for microbial culture or fluid drainage, catheter positioning to collect infectious material from the cavity and perform irrigation of the infected field or injection of iodine contrast when the suspicion of aortoenteric fistula exists. PMID:24518072

  2. Extending AAA operational model for profile-based access control in ethernet-based Neutral Access Networks

    NARCIS (Netherlands)

    J. Matias; E. Jacob; Y. Demchenko; C. de Laat; L. Gommans

    2010-01-01

    Neutral Access Networks (NAN) have appeared as a new model to overcome some restrictions and lack of flexibility that are present currently in broadband access networks. NAN brings new business opportunities by opening this market to new stakeholders. Although the NAN model is accepted, there are so

  3. The AAA-ATPase molecular chaperone Cdc48/p97 disassembles sumoylated centromeres, decondenses heterochromatin, and activates ribosomal RNA genes

    OpenAIRE

    Mérai, Zsuzsanna; Chumak, Nina; García-Aguilar, Marcelina; Hsieh, Tzung-Fu; Nishimura, Toshiro; Schoft, Vera K.; Bindics, János; Ślusarz, Lucyna; Arnoux, Stéphanie; Opravil, Susanne; Mechtler, Karl; Zilberman, Daniel; Fischer, Robert L.; Tamaru, Hisashi

    2014-01-01

    Centromeres are the fundamental unit required for segregation of chromosomes during mitosis and meiosis, and they are defined by the centromere-specific histone H3 variant (CenH3)/centromere protein A (CENP-A). In contrast to the relatively well-known process of de novo assembly of CenH3 at centromeres, little is known of how CenH3 is actively removed, leading to centromere disassembly, an essential biological process during the life of a cell. This study describes the process of centromere d...

  4. The Hetero-Hexameric Nature of a Chloroplast AAA+ FtsH Protease Contributes to Its Thermodynamic Stability

    Science.gov (United States)

    Ziv, Tamar; Adam, Zach; Prag, Gali

    2012-01-01

    FtsH is an evolutionary conserved membrane-bound metalloprotease complex. While in most prokaryotes FtsH is encoded by a single gene, multiple FtsH genes are found in eukaryotes. Genetic and biochemical data suggest that the Arabidopsis chloroplast FtsH is a hetero-hexamer. This raises the question why photosynthetic organisms require a heteromeric complex, whereas in most bacteria a homomeric one is sufficient. To gain structural information of the possible complexes, the Arabidopsis FtsH2 (type B) and FtsH5 (type A) were modeled. An in silico study with mixed models of FtsH2/5 suggests that heteromeric hexamer structure with ratio of 4∶2 is more likely to exists. Specifically, calculation of the buried surface area at the interfaces between neighboring subunits revealed that a hetero-complex should be thermodynamically more stable than a homo-hexamer, due to the presence of additional hydrophobic and hydrophilic interactions. To biochemically assess this model, we generated Arabidopsis transgenic plants, expressing epitope-tagged FtsH2 and immuno-purified the protein. Mass-spectrometry analysis showed that FtsH2 is associated with FtsH1, FtsH5 and FtsH8. Interestingly, we found that ‘type B’ subunits (FtsH2 and FtsH8) were 2–3 fold more abundant than ‘type A’ (FtsH1 and FtsH5). The biochemical data corroborate the in silico model and suggest that the thylakoid FtsH hexamer is composed of two ‘type A’ and four ‘type B’ subunits. PMID:22558304

  5. The hetero-hexameric nature of a chloroplast AAA+ FtsH protease contributes to its thermodynamic stability.

    Directory of Open Access Journals (Sweden)

    Ofer Moldavski

    Full Text Available FtsH is an evolutionary conserved membrane-bound metalloprotease complex. While in most prokaryotes FtsH is encoded by a single gene, multiple FtsH genes are found in eukaryotes. Genetic and biochemical data suggest that the Arabidopsis chloroplast FtsH is a hetero-hexamer. This raises the question why photosynthetic organisms require a heteromeric complex, whereas in most bacteria a homomeric one is sufficient. To gain structural information of the possible complexes, the Arabidopsis FtsH2 (type B and FtsH5 (type A were modeled. An in silico study with mixed models of FtsH2/5 suggests that heteromeric hexamer structure with ratio of 4:2 is more likely to exists. Specifically, calculation of the buried surface area at the interfaces between neighboring subunits revealed that a hetero-complex should be thermodynamically more stable than a homo-hexamer, due to the presence of additional hydrophobic and hydrophilic interactions. To biochemically assess this model, we generated Arabidopsis transgenic plants, expressing epitope-tagged FtsH2 and immuno-purified the protein. Mass-spectrometry analysis showed that FtsH2 is associated with FtsH1, FtsH5 and FtsH8. Interestingly, we found that 'type B' subunits (FtsH2 and FtsH8 were 2-3 fold more abundant than 'type A' (FtsH1 and FtsH5. The biochemical data corroborate the in silico model and suggest that the thylakoid FtsH hexamer is composed of two 'type A' and four 'type B' subunits.

  6. EFEITO DO ESPAÇAMENTO E DESBASTE NA PRODUÇÃO DA BANANEIRA DO CULTIVAR “MAÇÔ (Musa sp EFFECT OF THE SPACING AND THINNING IN BANANA (Musa sp. "MAÇÃ" CULTIVAR PRODUCTION

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    Lincoln F. Zica

    2007-09-01

    Full Text Available

    O presente trabalho teve como objetivo encontrar um espaçamento mais adequado, para conduzir os bananais do cultivar “maçã”, nas condições edáficas do Estado de Goiás. A experiência foi conduzida em solos sob cerrado, no município de Goiânia. Os seis tratamentos utilizados consistiram nas combinações de 3 (três espaçamentos com dois sistemas de condução, i.e. com e sem desbaste. A análise estatística dos dados obtidos demonstrou não haver interação desbaste x espaçamento. Com base nos resultados e observações feitas durante a fase experimental, tiraram-se as seguintes conclusões: a com o aumento da densidade de touceiras por unidade de área, há um aumento tanto do piso como do número de frutos produzidos; b O uso do desbaste não altera a produtividade, nas condições do presente experimento; c com a redução da área por touceira de 36 m² para 9 m², há um aumento na produtividade, sem prejudicar o tamanho dos frutos.

    The purpose of the present work was to determine a more adequate spacing for growing bananas (Maçã in natural conditions of the state of Goiás. The experiment was carried out in “cerrado” soils in Goiânia county. The six treatments used consisted of the combination of three (3 spacings, with and without pruning. The statistical analysis of the obtained data demonstrated that there was no interaction between pruning and spacing. The results obtained and the observations made during the spacing. The results obtained and the observations made during the experimental phase led to the following conclusions: 1. with the increase in density of the groups of shoots per unit of area, there is an increase in weight as well as an increase in the amount of fruit produced. 2. within the norms of this experiment, the use of pruning doesn’t alter productivity. 3. with the reduction of area from 36 to 9 m², for each group of’ shoots, there is an increase in productivity, without diminishing the size of the fruits.

  7. Características pós-colheita de frutos de genótipos de bananeira (Musa spp. Post-harvest fruit characteristics of different banana (Musa spp. genotypes

    Directory of Open Access Journals (Sweden)

    Reginaldo Conceição Cerqueira

    2002-12-01

    Full Text Available O conhecimento das características pós-colheita de novos híbridos de bananeira é uma importante informação que auxilia o melhorista em sua tomada de decisão. Este trabalho teve como objetivo avaliar genótipos de bananeira com relação às características de pós-colheita e resistência ao desprendimento do fruto. Foram utilizados frutos da segunda penca de 16 híbridos (Calipso, Bucaneiro, Ambrosia, YB42-21, PV42-53, PV42-68, PV42-81, PV42-85, PV42-129, PV42-142, PV42-143, ST12-31, ST42-08, PV03-44, FHIA-03 e SH 3640 e quatro cultivares (Pacovan, Prata Comum, Nam e Figue Pomme Naine, que foram avaliadas quanto ao peso, comprimento, circunferência, relação polpa/casca, resistência ao desprendimento, firmeza, sólidos solúveis totais (SST, acidez total titulável (ATT, relação SST/ATT e tempo de amadurecimento. Empregou-se um delineamento inteiramente casualizado, com cinco repetições para todas as características, exceto para a firmeza do fruto em que se utilizaram dez repetições. A unidade experimental foi constituída de um único fruto. Os dados foram submetidos à analise de variância e as médias comparadas pelo Teste Scott & Knott, a 5% de probabilidade. A cultivar Nam apresentou a menor acidez titulável e a maior relação SST/ATT, enquanto a maior firmeza do fruto foi apresentada pelos híbridos PV42-81 e PV42-53. Os frutos mais pesados foram do híbrido SH 3640, e os mais compridos foram dos híbridos Bucaneiro e PV42-81. Os híbridos PV42-85 e ST42-08 apresentaram maior resistência ao desprendimento, não diferindo da 'Pacovan'. No entanto, superaram a 'Prata Comum', que obteve a menor resistência. Os genótipos 'Nam', PV42-143 e PV42-129 apresentaram maior tempo de maturação após a colheita quando comparados, principalmente, à 'Prata Comum'. A 'Nam' deve ser considerada como uma nova alternativa na produção de banana. O SH3640, híbrido da Prata Anã, pode ser recomendado como nova cultivar. Os híbridos da Prata São Tomé (ST42-08 e Pacovan (PV42-85, PV42-81, PV42-68, PV42-53, PV42-129 e PV42-143 podem substituir as cultivares Prata Comum e Pacovan nos sitemas produção.The knowledge of the post-harvest characteristics of new banana hybrids is an important information that helps the breeder in making his decision. This work has the objective to evaluate banana genotypes in relation to their post-harvest characteristics and resistance to fruit drop. Fruits of the second hand of 16 hybrids (Calipso, Bucaneiro, Ambrosia, YB42-21, PV42-53, PV42-68, PV42-81, PV42-85, PV42-129, PV42-142, PV42-143, ST12-31, ST42-08, PV03-44, FHIA-03 and SH 3640 and four cultivars (Pacovan, Prata Comum, Nam and Figue Pomme Naine were evaluated according to their weight, length, circumference, pulp/peel ratio, resistance to fruit drop, firmness, total soluble solids (TSS, total titratable acidity (TTA, TSS/TTA ratio and maturation period. A completely randomized experimental design was used, with five replications, except fruit firmness with ten replications, each unit was constituted by one fruit. The data were submitted to the analysis of variance and the means were compared by the Scott & Knott test at 5% of probability. The cultivar Nam had the lowest TTA and the highest TSS/TTA ratio, while the highest fruit firmness was observed in PV42-81 and PV42-53. The SH 3640 produced the heaviest fruits, and Bucaneiro and PV42-81 produced the longest ones. PV42-85 and ST42-08 showed high resistance to fruit drop, with no statistical difference from 'Pacovan'. 'Prata Comum' showed the lowest resistance. The genotypes ' Nam', PV42-143 and PV42-129 showed longer shelf life when compared to 'Prata Comum'. 'Nam' genotype can be considered as alternative in banana production. SH3640 the Prata dwarf hybrid, might be considered as a new cultivar. Hybrids from Prata São Tomé (ST42-08 and from Pacovan (PV42-68, PV42-81, PV42-85, PV42-129 e PV42-143 can replace Prata Comum and Pacovan in the banana production system.

  8. REQUERIMIENTOS HÍDRICOS DEL PLÁTANO DOMINICO- HARTÓN (Musa AAB SIMMONDS EN LA REGIÓN SANTÁGUEDA (PALESTINA, CALDAS HYDRIC REQUIREMENTS OF PLANTAIN DOMINICO-HARTON (Musa AAB SIMMONDS INTHE SANTAGUEDA REGION (PALESTINA, CALDAS

    Directory of Open Access Journals (Sweden)

    Ángela María Castaño

    Full Text Available El presente estudio, se desarrolló en la granja Montelindo, de la Universidad de Caldas, con el fin de establecer los requerimientos hídricos del cultivo del plátano, con base en el cálculo de la evapotranspiración de referencia, calculada por el método de Penman-Monteith y la evapotranspiración del cultivo, según el estado fenológico de la planta. Se sembraron plantas de plátano Dominico-Hartón, a 2m entre plantas y 3m, entre surcos y, oportunamente, se efectuaron las prácticas agronómicas necesarias. Al inicio del estudio, se determinaron la capacidad de campo, el punto de marchitez permanente y la lámina de agua aprovechable. Semanalmente, se hicieron registros de los elementos climáticos, el crecimiento de las plantas y la tensión de humedad del suelo, utilizando tensiómetros ubicados a 30,48cm y 45,72cm de profundidad. Se estableció que en la región Santágueda, las plantas de Dominico-Hartón, bajo condiciones adecuadas de cultivo, requieren 1.141mm de agua, durante un ciclo del cultivo; que el consumo de agua aumenta gradualmente a medida que el cultivo avanza en su desarrollo, siendo la floración la época de mayor uso de agua y la más crítica, en cuanto al déficit hídrico. También, se encontró que la altura de la planta, la emisión de hojas y el diámetro del pseudotallo tienen relación directa con la evapotranspiración del cultivo.The present study was developed at the farm 'Montelindo' of the University of Caldas in order to establish the hydric requirements of the plantain crop, based on the evapotranspiration calculated by Penman-Monteith method and the crop evapotranspiration according to the phonological stage of the plant. Plants of Dominico-Harton plantain were planted at 2m between plants and 3m between rows and the necessary agronomic practices were applied on time. At the beginning of the study the field capacity, the permanent wilting point and the usable water table were determined. Weekly, registers of climatic elements, plants growth and soil humidity tension with tensiometers located at 40, 48 and 45, 72 inches deep, were made. It was established that at the Santagueda region the Dominico-Harton plants, under appropriated culture conditions, require 1.141mm of water during a culture cycle; that water consumption increases as the development of the crop advances, being the flowering period the time of highest water consumption and also the most critical one in relation to water deficit. It was also found that plant height, leaves emission and pseudostem diameter have a direct relation with crop evapotranspiration.

  9. Processamento térmico de purê de banana (Musa cavendishii, Lamb. em embalagens flexíveis esterilizáveis Thermal processing of banana puree (Musa cavendishii, Lamb. in retortale pouches

    Directory of Open Access Journals (Sweden)

    Celso Duarte CARVALHO FILHO

    1997-12-01

    Full Text Available O objetivo deste trabalho foi estabelecer as condições de processo térmico de purê de banana, variedade "nanica", em bolsas esterilizáveis porção individual (130 x 170mm. Os frutos foram selecionados, aquecidos a 98°C/5min. para inativação enzimática, separadas as sementes e fibras num despolpador com malha de 0,08cm de diâmetro e depois o purê foi embalado nas bolsas sob um vácuo de 25mmHg, resultando em média 2,7ml de ar residual por bolsa. Em seguida as bolsas foram processadas sem agitação e em posição horizontal numa autoclave horizontal, a 115°C/158KPa, com imersão total de água quente. O tempo de manutenção do processo foi de 7,5min. Inicialmente foi utilizado o Clostridium butyricum como microrganismo alvo do processo, entretanto, observou-se que esta bactéria apresentara uma resistência térmica em purê de banana (pH 4,6 um pouco menor que o valor estimado para o Clostridium botulinum, D115=0,183min. e D115=0,236min respectivamente, sendo assim, o C. botulinum foi escolhido como microrganismo alvo da esterilização do produto. O valor de F121,1°C aplicado foi de 0,64min. para causar 12 reduções decimais, calculado segundo PFLUG (1985 para um pH de 4,6, e verificado pelo método geral. Este valor somente foi considerado para a fase de aquecimento, deixando a letalidade do resfriamento (0,34min., que foi realizada com água à temperatura ambiente, como segurança de processo. Nos testes de penetração de calor foram encontrados os valores médio de fh=6,8min; jh=0,48; fc=17,4min. e jc=1,3 demonstrando assim, que o produto é altamente condutivo. Nenhuma alteração no produto nem nas embalagens foi notada após a aplicação do ensaio de esterilidade comercial em 36 bolsas processadas contendo purê de banana, confirmando assim, a eficácia do tratamento térmico aplicado.The aim of this work was to establish thermal processing conditions for banana puree in retor pouches (130 x 170mm. The fruits were selected, blanched in boiling water for 5min., finished (0,033 inch mesh and vacuum-packaged in 25mmHg with 2,7ml residual air/pouch. After the pouches were processed without agitation in horizontal position at 115°C/158 Kpa over-pressure in water total immersion. The holding process time was 7,5min. Initially Clostridium butyricum was used as target organism for the thermal process, however the banana puree is situated in pH limit area of (4,6 for low acid foods, favouring the Clostridium botulinum growth. Its heat resistance was estimated through of the equation 1 (PFLUG, 1985 and compared with C. butyricum. Though the D115°C values for C. butyricum and C. botulinum were similar (0,183 min and 0,236 min, respectively, C. botulinum higher compared to C. butyricum. The F121,1°C applied was 0,64 min to obtain 12 decimal reductions calculated by PFLUG (1985 and verified by the general method. This value was only considerated for the heating phase. The value of F121,1°C = 0,27 min (29,5% determinated for cooling phase, was used as safety factor. Average values of the heating rate rate parameter fh and the lag factor were: fh=6,8min. and jh=0,48. For the cooling phase the average values were: fc=17,4min. and jc=1,3. After 15 days/37°C incubation, no abnormal conditions were noted in the 36 processed pouches. This is an indicative that the process was satisfactory from the microbiological standpoint.

  10. EVALUATION OF GASTRIC ANTIULCEROGENIC ACTION OF PLANTAIN BANANA ( MUSA SAPIENTUM VAR. PARADISIACA IN ASPIRIN PLUS PYLORUS LIGATED ALBINO RATS

    Directory of Open Access Journals (Sweden)

    K.R. Tandel* and B.K. Shah

    2012-11-01

    Full Text Available Objectives: To assess the effect of unripe dried banana powder in experimentally induced gastric ulcers and effect on gastric acid secretion. To evaluate the antiulcerogenic effect of plantain banana of Gujarat as a part of evaluation of impact of biological variables on this activity. Materials and methods: Total of 24 albino rats of either sex weighing between 150-250 gm were randomly divided into 4 groups. Each group has 6 no. of rats. The first group received placebo (distilled water, the second, third & forth group received 0.5gm/kg, 1gm/kg and 2mg/kg of banana powder respectively. Banana powder was given as suspension at fixed time (3 times in a day for two days and animals were kept for fasting for another 48hrs. On 5th day, the animals were sacrificed after 7 hrs and stomach were removed for examination and gastric juice samples were collected to analyze volume and acidity.Results: Orally administered banana powder in the dose of 2gm/kg caused a statistically significant decrease in aspirin with pyloric ligation induced ulcers in rats without significantly decrease in secretary activity.Conclusion: It can be concluded from these results that vegetable plantain banana has antiulcerogenic and mucosal protective actions, but it has no antisecretory activity.

  11. ESTUDIO PRELIMINAR DEL COMPORTAMIENTO REOLOGICO BAJO COMPRESIÓN UNIDIRECCIONAL DE LA PULPA DE PLÁTANO (Musa AAB Simmonds

    Directory of Open Access Journals (Sweden)

    HÉCTOR JOSÉ CIRO VELÁSQUEZ

    2008-01-01

    Full Text Available Pulpa de plátano Dominico-Hartón fue sometida a ensayos de compresión unidireccional usando un analizador de textura TA-XT2i. Las curvas de flujo y las propiedades reológicas del producto a condición de falla como esfuerzo, deformación y tenacidad fueron determinadas. La pulpa fue sometida a dos direcciones de carga (longitudinal y transversal en tres posiciones a lo largo del eje longitudinal del producto: inicial, medio y final. Los análisis estadísticos mostraron que el material es altamente anisotrópico, donde la dirección de carga y la posición dentro de la material son efectos significativos en la respuesta reológica del producto.

  12. ESTUDIO PRELIMINAR DEL COMPORTAMIENTO REOLOGICO BAJO COMPRESIÓN UNIDIRECCIONAL DE LA PULPA DE PLÁTANO (Musa AAB Simmonds)

    OpenAIRE

    HÉCTOR JOSÉ CIRO VELÁSQUEZ; FABIAN ARMANDO MONROY LAITON; ELKIN ALONSO CORTÉS MARÍN

    2008-01-01

    Pulpa de plátano Dominico-Hartón fue sometida a ensayos de compresión unidireccional usando un analizador de textura TA-XT2i. Las curvas de flujo y las propiedades reológicas del producto a condición de falla como esfuerzo, deformación y tenacidad fueron determinadas. La pulpa fue sometida a dos direcciones de carga (longitudinal y transversal) en tres posiciones a lo largo del eje longitudinal del producto: inicial, medio y final. Los análisis estadísticos mostraron que el material es altame...

  13. Biochemical and In-silico Studies on Pectin Methylesterase from G9 Variety of Musa acuminata for Delayed Ripening

    OpenAIRE

    Verma, Charu; R.K, Singh; Singh, Ram B; Mishra, Sanjay

    2015-01-01

    Ripening of fruit is a very important process but in some fruits early ripening leads to a great damage during long distance transportation. There are various biochemical changes taking place during the phase of ripening of fruit such as changes in respiration, aroma, flavor, ethylene production and activity of cell wall degrading enzymes. Some important cell wall degrading enzymes are Polygalacturonase (PG), Pectin methylesterase (PME), Pectin lyase, RGase. PME is known to act as a cell wall...

  14. Quantitative Proteomic Analysis Reveals that Antioxidation Mechanisms Contribute to Cold Tolerance in Plantain (Musa paradisiaca L.;ABB Group) Seedlings

    Institute of Scientific and Technical Information of China (English)

    Qiaosong Yang; Junhua Wu; Chunyu Li; Yuerong Wei; Ou Sheng; Chunhua Hu; Ruibin Kuang

    2012-01-01

    Banana and its close relative,plantain are globally important crops and there is of considerable interest in optimizing their cultivation.Plantain has superior cold tolerance compared to banana and a thorough understanding of the molecular mechanisms and responses of plantain to cold stress has great potential value for developing cold tolerant banana cultivars.In this study,we used iTRAQ-based comparative proteomic analysis to investigate the temporal responses of plantain to cold stress.Plantain seedlings were exposed for 0,6 and 24 h of cold stress at 8℃ and subsequently allowed to recover for 24 h at 28℃.A total of 3,477 plantain proteins were identified,of which 809 showed differential expression from the three treatments.The majority of differentially expressed proteins were predicted to be involved in oxidation-reduction,including oxylipin biosynthesis,while others were associated with photosynthesis,photorespiration and several primary metabolic processes,such as carbohydrate metabolic process and fatty acid beta-oxidation.Western blot analysis and enzyme activity assays were performed on 7 differentially expressed,cold-response candidate plantain proteins in order to validate the proteomics data.Similar analyses of the 7 candidate proteins were performed in cold-sensitive banana to examine possible functional conservation and to compare the results to equivalent responses between the two species.Consistent results were achieved by Western blot and enzyme activity assays,demonstrating that the quantitative proteomics data collected in this study are reliable.Our results suggest that an increase of antioxidant capacity through adapted ROS scavenging capability,reduced production of ROS and decreased lipid peroxidation contribute to molecular mechanisms for the higher cold tolerance in plantain.To the best of our knowledge,this is the first report of a global investigation on molecular responses of plantain to cold stress by proteomic analysis.

  15. Physicochemical properties of stiff dough ‘amala’ prepared from plantain (Musa Paradisca) flour and Moringa (Moringa oleifera) leaf powder

    OpenAIRE

    Karim, Olayinka; Kayode, Rowland; Oyeyinka, Samson; Oyeyinka, Adewumi

    2015-01-01

    Plantains are a good source of resistant starch and are currently being used in the dietary management of diabetes when consumed in the unripe stage. They can be used in making amala, a stiff dough commonly consumed in some parts of Africa including Nigeria. The addition of fortificant to foods may affect product composition and functionality; therefore this study investigated the effect of Moringa oleifera leaf powder at varying concentrations (0, 0.5, 1.0, 1.5, 2.0 and 2.5 %) on pasting and...

  16. Effect of Reinforcement of Hydrophobic Grade Banana (Musa ornata Bark Fiber on the Physicomechanical Properties of Isotactic Polypropylene

    Directory of Open Access Journals (Sweden)

    Md. Mamunur Rashid

    2016-01-01

    Full Text Available This research studied the physicomechanical as well as morphological properties of alkali treated (NaOH and KMnO4 and untreated banana bark fiber (BBF reinforced polypropylene composites. A detailed structural and morphological characterization was performed using Fourier transform infrared spectroscopy (FTIR, scanning electron microscopy (SEM, and mechanical properties testing (tensile strength, flexural strength, and microhardness. Chemical treatments improved the hydrophobic property of the fiber and it is found to be better for KMnO4 treatment. Composites with 0, 5, 10, and 15 wt.% loadings were then compared for water uptake studies and revealed that KMnO4 treated fiber composites absorb less water compared to others. KMnO4 treatment with 15% fiber loading improved the tensile strength, flexural strength, and microhardness of the composites compared to raw and NaOH treated fiber loadings. TGA analysis also shows onset temperature at 400~500°C that is associated with the decomposition of the banana fibers constituents including lignin, cellulose, and hemicelluloses which suggests better thermomechanical stability. All of the values suggest that 15% KMnO4 treated banana bark fiber (BBF/PP composites were found to be better than those of the raw and NaOH treated ones.

  17. PRELIMINARY SCREENING RESISTANCE OF Musa GERMPLASMS FOR BANANA BUNCHY TOP DISEASE IN PURWODADI BOTANIC GARDEN, PASURUAN, EAST JAWA

    Directory of Open Access Journals (Sweden)

    Lia Hapsari

    2012-07-01

    Full Text Available Banana Bunchy Top Disease (BBTD caused by Banana Bunchy Top Virus (BBTV is one of the most serious banana diseases, constraint and devastate to the local and regional banana production. Some of banana cultivars were more readily infected by the virus, but considering no cultivars is resistant. The incidences of BBTD and the type and severity symptoms in natural conditions without any artificial inoculation were recorded. The observations results on 2009 showed that 12.14% of the total accessions indicated positively infected BBTD. The BBTD incidences were increased rapidly about twofolds per year, 28.57% in October 2011 and 54.29% in October 2011. The spread and development of BBTD is optimum at humid, high rainfall intensity and temperatures, high soil fertility and also low light intensity or shaded. The latest monitoring on February 2012 (62.14% comprises of 64 accessions were positively infected and 39 accessions were symptomless. Those symptomless accessions were initially indicated as tolerant banana cultivars to BBTD e.g. Pisang Kepok (BBB, Pisang Sobo (BBB, Pisang Bandung (ABB, Pisang Nangka (AAB, Pisang Candi (AAB and  Pisang Raja Marto (AAB. These preliminary results are in agreement with several previous studies indicated that genotypes with one or two B genomes tend to be more tolerant to BBTD. However several cultivars which known tend to BBTD tolerant are indicated susceptible to Blood Disease. Some cultivars also showing complication symptoms both BBTD and Blood Disease.

  18. Genome-Wide Identification and Expression Analysis of Homeodomain Leucine Zipper Subfamily IV (HDZ IV) Gene Family from Musa accuminata

    OpenAIRE

    Pandey, Ashutosh; Misra, Prashant; Alok, Anshu; Kaur, Navneet; Sharma, Shivani; Lakhwani, Deepika; Asif, Mehar H.; Tiwari, Siddharth; Trivedi, Prabodh K.

    2016-01-01

    The homeodomain zipper family (HD-ZIP) of transcription factors is present only in plants and plays important role in the regulation of plant-specific processes. The subfamily IV of HDZ transcription factors (HD-ZIP IV) has primarily been implicated in the regulation of epidermal structure development. Though this gene family is present in all lineages of land plants, members of this gene family have not been identified in banana, which is one of the major staple fruit crops. In the present w...

  19. Genome wide identification and expression analysis of Homeodomain leucine zipper subfamily IV (HDZ IV) gene family from Musa accuminata

    OpenAIRE

    Ashutosh ePandey; Prashant eMisra; Anshu eAlok; Navneet eKaur; Shivai eSharma; Deepika eLakhwani; Mehar H Hasan; Siddhartha eTiwari; Prabodh KUMAR Trivedi

    2016-01-01

    The homedodomain zipper family (HD-ZIP) of transcription factors is present only in plants and plays important role in the regulation of plant-specific processes. The subfamily IV of HDZ transcription factors (HD-ZIP IV) has primarily been implicated in the regulation of epidermal structure development. Though this gene family is present in all lineages of land plants, members of this gene family have not been identified in banana, which is one of the major staple fruit crops. In the present ...

  20. ESTUDO DO PRIMEIRO CICLO PRODUTIVO DA BANANEIRA `NANICÃO' (Musa sp. DESENVOLVIDA A PARTIR DE DIFERENTES TIPOS DE MUDA

    Directory of Open Access Journals (Sweden)

    SCARPARE FILHO J.A.

    1998-01-01

    Full Text Available Para avaliar o primeiro ciclo produtivo de bananeira `Nanicão' foram utilizados 5 tipos de muda: "chifrinho" e "chifrão" (brotações laterais com folhas lanceoladas, "guarda-chuva" (brotações separadas da planta mãe, com folhas normais, pedaços de rizoma e mudas micropropagadas. Foram avaliados o peso dos cachos, o número de pencas, o número de frutos por cacho e número de dias decorridos do plantio até a colheita. Os melhores desempenhos foram obtidos com as mudas "chifrinho" e "chifrão", que apresentaram maior equilíbrio das variáveis estudadas. As mudas constituidas de pedaços de rizoma, embora com uma produção alta, mostraram-se tardias no primeiro ciclo. O pior desempenho foi verificado nas mudas micropropagadas, com produções inferiores aos demais tratamentos, apresentando primeiro ciclo tardio e alta taxa de variação somaclonal.