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Sample records for banana cultivars musa

  1. Anatomy and morphology character of five Indonesian banana cultivars (Musa spp. of different ploidy level

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    ISSIREP SUMARDI

    2010-04-01

    Full Text Available Sumardi I, Wulandari M (2011 Anatomy and morphology character of five Indonesian banana cultivars (Musa spp. of different ploidy level. Biodiversitas 12: 167-175. In Indonesia there are many cultivars of banana, and some of them produce edible fruits. Beside their morphology, the character which necessary as a tool for classification is anatomical character. The aim of this research were to describe the anatomical character and morphology of fives Indonesian banana cultivars based on their level of ploidy. The cultivars were collected from Banana Germplasm Plantation, Yogyakarta District, Indonesia. The samples of roots, rhizome, and leaf were collected from five banana cultivars i.e.: Musa acuminata cv Penjalin, M.balbisiana cv Kluthuk warangan, M.acuminata cv Ambon warangan, M.paradisiaca cv Raja nangka , and M. paradisiaca cv Kluthuk susu. For anatomy observation samples were prepared using paraffin method, stained with 1% safranin in 70% ethanol. To observe the structure of stomata and epidermis surface, slide were prepared using modification of whole mount method. Slides were observed using Olympus BHB microscope completed with Olympus camera BM-10A. Stem and leaf morphology character of diploid level (AA and BB genome is different with triploid level (AAA, AAB, and ABB genome. Anatomy and morphology character of root and rhizome of banana in diploid level (AA and BB genome and triploid level (AAA, AAB, and ABB genome is quite similar. Distribution of stomata is found in leaf and pseudostem. Stomata is found in adaxial and abaxial epidermis layer. The size of guard cells in triploid cultivars was longer than that diploid cultivars. The root composse of epidermis layer, cortex and cylinder vascular of five cultivar’s root show anomalous structure. Rhizome consist of peripheric and centre zone. Anatomically, this was no differences in the rizome structur among five banana cultivars. The row of vascular bundles act as demarcation area

  2. The Quest for Golden Bananas: Investigating Carotenoid Regulation in a Fe'i Group Musa Cultivar.

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    Buah, Stephen; Mlalazi, Bulukani; Khanna, Harjeet; Dale, James L; Mortimer, Cara L

    2016-04-27

    The regulation of carotenoid biosynthesis in a high-carotenoid-accumulating Fe'i group Musa cultivar, "Asupina", has been examined and compared to that of a low-carotenoid-accumulating cultivar, "Cavendish", to understand the molecular basis underlying carotenogenesis during banana fruit development. Comparisons in the accumulation of carotenoid species, expression of isoprenoid genes, and product sequestration are reported. Key differences between the cultivars include greater carotenoid cleavage dioxygenase 4 (CCD4) expression in "Cavendish" and the conversion of amyloplasts to chromoplasts during fruit ripening in "Asupina". Chromoplast development coincided with a reduction in dry matter content and fruit firmness. Chromoplasts were not observed in "Cavendish" fruits. Such information should provide important insights for future developments in the biofortification and breeding of banana.

  3. Characterisation of metabolic profile of banana genotypes, aiming at biofortified Musa spp. cultivars.

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    Borges, Cristine Vanz; Amorim, Vanusia Batista de Oliveira; Ramlov, Fernanda; Ledo, Carlos Alberto da Silva; Donato, Marcela; Maraschin, Marcelo; Amorim, Edson Perito

    2014-02-15

    The banana is an important, widely consumed fruit, especially in areas of rampant undernutrition. Twenty-nine samples were analysed, including 9 diploids, 13 triploids and 7 tetraploids, in the Active Germplasm Bank, at Embrapa Cassava & Fruits, to evaluate the bioactive compounds. The results of this study reveal the presence of a diversity of bioactive compounds, e.g., catechins; they are phenolic compounds with high antioxidant potential and antitumour activity. In addition, accessions with appreciable amounts of pVACs were identified, especially compared with the main cultivars that are currently marketed. The ATR-FTIR, combined with principal components analysis, identified accessions with distinct metabolic profiles in the fingerprint regions of compounds important for human health. Likewise, starch fraction characterisation allowed discrimination of accessions according to their physical, chemical, and functional properties. The results of this study demonstrate that the banana has functional characteristics endowing it with the potential to promote human health.

  4. Aproveitamento de residuo da banana (casca e coroa), cultivar nanição, Musa cavendishu, para uso em panificação

    OpenAIRE

    1997-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciencias Agrarias Visando o aproveitamento dos resíduos da banana (casca e coroa), cultivar nanicão, Musa cavendishii L., foi proposta sua utilização como farinha para panificação. O resíduo foi submetido ao processo de branqueamento, secagem em estufa e moagem. A farinha obtida foi analisada quanto as características físico-química e microbiológica, e calculado seu valor calórico. Os pães foram elaborados com as fa...

  5. ARMAZENAMENTO E MATURAÇÃO DE BANANAS DO CULTIVAR PRATA (Musa sp EM SACOS DE POLIETILENO CONTENDO ABSORVENTE DE ETILENO STORAGE AND MATURATION OF BANANA (Musa sp. “PRATA” CULTIVAR IN POLYETHYLENE BAGS CONTAINING ETHYLENE ABSORBENT

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    Lincoln F. Zica

    2007-09-01

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    Este trabalho teve como objetivo estudar um meio adequado de conservação de banana “Prata” (Musa sp., para as condições de mercado interno, utilizando polietileno como modificador do meio envolvente e como embalagem permanente do produtor ao consumidor. Para isto, utilizaram-se os seguintes tratamentos: Testemunha, sem embalagem; Plástico perfurado; Plástico perfurado, contendo absorvente; Plástico sem perfuração; Plástico sem perfuração, contendo absorvente. As bananas tratadas, foram acondicionadas em câmara com temperatura constante de 22 ± 1°C e umidade relativa de 90 ± 3%. Uma vez por dia era feita a leitura da cor da casca e a ventilação de câmara. Com auxílio das análises estatísticas dos dados obtidos concluiu-se que: a. Uso de absorvente de etileno dentro da embalagem plástica, sem perfuração, atrasa o início do amadurecimento, mas não impede que os frutos se tornem imprestáveis para o consumo. b. A melhor embalagem encontrada para ser usada do produtor ao consumidor foi a de plástico perfurado sem absorvente de etileno, que dá um ganho de aproximadamente cinco dias de conservação à temperatura de 22° C.

    This experiment had as its objective the study of an adequate way to conserve the banana “Prata” (Musa sp. in order to meet conditions of the national market. Poliethylene packaging from the producer to the consumer. For this, the following treatments were used: no packaging, perforated plastic, imperforated plastic containing an absorvent, imperforated plastic, imperforated plastic containing an absorvent. The treated banana were stored in a container with a constant temperature of 22 ± 1°C and a relative humidity of 90 ± 3%. Once a day a reading was taken of the skin color and the room was ventilated. With the help of statistical analysis of the obtained

  6. New microsatellite markers for bananas (Musa spp).

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    Amorim, E P; Silva, P H; Ferreira, C F; Amorim, V B O; Santos, V J; Vilarinhos, A D; Santos, C M R; Souza Júnior, M T; Miller, R N G

    2012-04-27

    Thirty-four microsatellite markers (SSRs) were identified in EST and BAC clones from Musa acuminata burmannicoides var. Calcutta 4 and validated in 22 Musa genotypes from the Banana Germplasm Bank of Embrapa-CNPMF, which includes wild and improved diploids. The number of alleles per locus ranged from 2 to 14. The markers were considered highly informative based on their polymorphism information content values; more than 50% were above 0.5. These SSRs will be useful for banana breeding programs, for studies of genetic diversity, germplasm characterization and selection, development of saturated genetic linkage maps, and marker assisted selection.

  7. EFEITO DA EMBALAGEM COM SACOS DE POLIETILENO NO COMPORTAMENTO DO AMIDO NA POLPA DE BANANA DO CULTIVAR PRATA (Musa sp. NO PERÍODO DE CONSERVAÇÃO E MATURAÇÃO EFFECT OF POLYETHYLENE PACKING BAGS ON THE STARCH BEHAVIOR IN THE CONSERVATION MATURATION PERIOD OF BANANA PULP CULTIVAR “PRATA” (Musa sp.

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    Lincoln F. Zica

    2007-09-01

    Full Text Available

    Neste trabalho estudou-se o comportamento do amido da polpa de banana do cultivar “Prata" (Musa sp., sob os seguintes tratamentos: Testemunha, sem embalagem; Plástico perfurado; Plástico perfurado, contendo absorvente; Plástico sem perfuração; Plástico, sem perfuração, contendo absorvente. As bananas tratadas, foram acondicionadas em uma câmara com temperatura constante de 22 ± 1°C e umidade relativa de 90 ± 3%. Uma vez por dia era feita a ventilação da câmara. De dois em dois dias, retiravam-se amostras de cada tratamento e faziam-se as análises. Com auxílio das análises estatísticas dos dados obtidos conclui-se que: a. A embalagem com polietileno atrasa o início da queda do amido, mas não impede a sua transformação. b. a percentagem de amido na polpa, permanece constante até que, em dado momento, que varia com os tratamentos, inicia a sua variação. c. O uso de absorvente de etileno dentro da embalagem plástica, sem perfuração, atrasa mais o início da queda do amido, mas não impede que os frutos se tornem imprestáveis para o consumo.

    In this experiment, the behavior of starch in the pulp of banana Prata (Musa sp., was studied under the following treatments: bananas without packing, perforated plastic, perforated plastic with an absorbent, unperforated plastic, unperforated plastic containing an absorbent. The treated bananas, were shelved at 22 ± 1°C and a relative humidity of 90 ± 3%. Every 2 days, samples of each treatment were collected and analysis were made. With the help of statistical analyses of the data, the following conclusions were reached: a. The packaging with polyethylene delays the starch breakdown, but does not impede its transformation. b. The percentage of starch of the pulp remains constant until a given moment, which varies with each treatment, when its

  8. Phenotypic and molecular characterization of Colletotrichum species associated with anthracnose of banana (Musa spp) in Malaysia.

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    Intan Sakinah, M A; Suzianti, I V; Latiffah, Z

    2014-05-09

    Anthracnose caused by Colletotrichum species is a common postharvest disease of banana fruit. We investigated and identified Colletotrichum species associated with anthracnose in several local banana cultivars based on morphological characteristics and sequencing of ITS regions and of the β-tubulin gene. Thirty-eight Colletotrichum isolates were encountered in anthracnose lesions of five local banana cultivars, 'berangan', 'mas', 'awak', 'rastali', and 'nangka'. Based on morphological characteristics, 32 isolates were identified as Colletotrichum gloeosporioides and 6 isolates as C. musae. C. gloeosporioides isolates were divided into two morphotypes, with differences in colony color, shape of the conidia and growth rate. Based on ITS regions and β-tubulin sequences, 35 of the isolates were identified as C. gloeosporioides and only 3 isolates as C. musae; the percentage of similarity from BLAST ranged from 95-100% for ITS regions and 97-100% for β-tubulin. C. gloeosporioides isolates were more prevalent compared to C. musae. This is the first record of C. gloeosporioides associated with banana anthracnose in Malaysia. In a phylogenetic analysis of the combined dataset of ITS regions and β-tubulin using a maximum likelihood method, C. gloeosporioides and C. musae isolates were clearly separated into two groups. We concluded that C. gloeosporioides and C. musae isolates are associated with anthracnose in the local banana cultivars and that C. gloeosporioides is more prevalent than C. musae.

  9. Avaliação de cultivares de bananeira (Musa sp na região de Selvíria-MS Evaluation of banana (Musa sp cultivars in Selvíria-MS region

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    Edicléia Aparecida da Silva

    2006-04-01

    Full Text Available O presente trabalho teve como objetivo avaliar o ciclo e a produção de sete cultivares de bananeira na região de Selvíria, Estado do Mato Grosso do Sul. O delineamento experimental utilizado foi do tipo blocos ao acaso, com sete tratamentos (Nanica; Mysore; Marmelo; Ouro-da-Mata; Ourinho; São Tomé e Prata e quatro repetições, num total de 28 parcelas. Os resultados obtidos permitiram as seguintes conclusões: A cultivar Mysore mostrou maior período do plantio ao florescimento, e as cultivares Nanica e São Tomé, menores. As cultivares Ouro-da-Mata e Ourinho apresentaram menor ciclo do plantio à colheita, enquanto as cultivares Mysore e Prata apresentaram ciclos maiores e iguais. As cultivares Marmelo e Nanica produziram cachos e pencas maiores, e as cultivares Ouro-da-Mata e Ourinho produziram cachos e pencas menores. A cultivar Mysore apresentou cacho com maior número de pencas, e a cultivar São Tomé, menor número. A cultivar Ourinho obteve maior número de frutos por penca, e a cultivar Marmelo, menor número. A cultivar Marmelo destacou-se com maior comprimento e diâmetro dos frutos da segunda penca. As melhores cultivares para esta região são Nanica e Marmelo, pois apresentaram bom desenvolvimento.The objective of this study was to evaluate the development and the production of seven banana cultivars in the region of Selvíria, Mato Grosso do Sul state. The experiment was conducted in random blocks, under seven different treatments (Nanica; Mysore; Marmelo; Ouro-da-Mata; Ourinho; São Tomé e Prata with four repetitions, in a total of 28 plots. The results led to the following conclusions: the period which goes from the planting to the flowering was longer for the Mysore cultivar and shorter for the Nanica and São Tomé cultivars. The development from the planting to the harvesting was shorter for the Ouro-da-Mata and Ourinho cultivars and equally longer for the Mysore and Prata cultivars. The Marmelo end Nanica cultivars

  10. Variação somaclonal em mudas micropropagadas de bananeira, cultivar Pacovan Somaclonal variation event on micropropagated Pacovan banana seedling (Musa spp. AAB group

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    Cynthia Christina Carvalho dos Santos

    2004-01-01

    Full Text Available A literatura tem relatado a ocorrência de variação somaclonal em plantas derivadas de cultura de tecidos. Neste trabalho, foi estudada a influência do número de subcultivos na indução de variantes em bananeira, cultivar Pacovan (Musa spp., grupo AAB. Ápices caulinares foram introduzidos e multiplicados in vitro, utilizando-se no estabelecimento o meio de cultura MS com adição de 2,5 mg.L-1 de BAP e nos subcultivos subseqüentes, o meio MS com 4,0 mg.L-1 de BAP para indução de brotações laterais. Foram obtidas gerações com diferentes números de subcultivos (3, 4, 5, 6, 7, 8 e 9, e comparadas com mudas obtidas de rizomas. Observaram-se as plantas obtidas, no total de 426, em condições de campo no Vale do Açu (RN. Para cálculo da porcentagem de variantes somaclonais, avaliaram-se as seguintes características morfológicas: estatura da planta, coloração e forma das folhas e conformação do cacho. Foram consideradas como variantes apenas as plantas cujos perfilhos também mostravam o mesmo tipo de variação. Constatou-se a ocorrência de variação somaclonal nas mudas provenientes dos tratamentos a partir de cinco subcultivos, atingindo-se o valor de 5,8% de variantes no nono subcultivo. O aumento da porcentagem de variação somaclonal com o do número de subcultivos indica o cuidado que as biofábricas devem ter em relação a esse parâmetro, desenvolvendo protocolos de micropropagação específicos para cada cultivar a ser comercializada.The occurrence of somaclonal variation for plants in developed from tissue culture is well documented in the literature. The present study evaluated the influence of numbers subculture in the induction of variants in Pacovan banana tree (Musa spp., AAB group. Apex stems were introduced and multiplied in vitro using culture media MS, with addition of 2.5 mg.L-1 of BAP. In subsequent subcultures, MS with 4.0 mg.L-1 of BAP was used to induce side buds. Results showed that plants were

  11. Estabelecimento e multiplicação in vitro de brotos no processo de micropropagação de cultivares de bananeira (Musa spp. Establishment and in vitro multiplication of banana (Musa spp. cultivars with the use of PVP (Polyvinylpyrrolidone

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    Hérica Santos de Oliveira

    2011-01-01

    Full Text Available A banana (Musa spp. é uma das frutas mais consumidas no mundo, e amplamente cultivada no Brasil, porém doenças como as sigatokas, negra e amarela, vêm reduzindo a sua produção. A disponibilização imediata de novas cultivares resistentes às principais doenças é limitada pela propagação convencional. A micropropagação é uma alternativa para a produção de mudas com qualidade fitossanitária e vegetativa, mas apresenta fatores que dificultam sua aplicação como a contaminação por fungos e bactérias, associada à oxidação dos explantes. O objetivo desse trabalho foi adaptar e/ou otimizar as etapas do processo de micropropagação para diferentes cultivares de bananeira, por meio do controle de oxidação, contaminação, e multiplicação de brotos, sendo utilizadas as cultivares Caipira (AAA, BRS Caprichosa (AAAB, Pacovan Ken (AAAB, Preciosa (AAAB, PV 03-76 (AAAB, Thap Maeo (AAB. No estudo foram utilizados o antibiótico sulfato de estreptomicina e o fungicida Opera® (BASF visando reduzir a contaminação in vitro provocada por bactérias e fungos, além do anti-oxidante PVP (polivinilpirrolidona para controlar a oxidação. Houve redução da contaminação com uso do sulfato de estreptomicina à concentração de 100 mg L-1 e da oxidação com PVP a 4 g L-1. Na fase de multiplicação de brotos, as cultivares apresentaram médias que variaram de 1,90 a 4,75 brotos/explante. A cultivar caipira (AAA destacou-se das demais com a maior taxa de multiplicação de brotos após três subcultivos, média de 41,50 brotos por rizoma.The banana (Musa spp is one of the most consumed fruits in the world and is widely consumed in Brazil, but diseases such as yellow and black sigatoka have been reducing its production. The immediate availability of new cultivars resistant to major diseases is limited by conventional propagation. The micropropagation, is an alternative for the production of seedlings with phytosanitarium and vegetative

  12. EFEITO DO PERÍODO DE ARMAZENAMENTO SOBRE A BROTAÇÃO DE MUDAS DE BANANEIRA DO CULTIVAR NANICÃO (Musa acuminata AAA EFFECT OF STORAGE TIME IN THE BUDDING OF BANANA ROOTSTOCK CULTIVAR NANICÃO (Musa acuminata AAA

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    Ronaldo Veloso Naves

    2007-09-01

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    A grande procura atual de mudas de bananeiras do cultivar Nanicão (Musa acuminata AAA em Goiás, principalmente na região do Mato Grosso Goiano, tem forçado o transporte de mudas a grandes distâncias. A maioria destas mudas são transportadas sob a forma de pedaços de rizoma com aproximadamente 1 Kg. Tem-se observado uma falha acima do previsto quando se utiliza este tipo de muda. O presente trabalho foi realizado para determinar o efeito do período de armazenamento sobre a brotação deste cultivar. Com base nos resultados concluiu-se que no período de armazenamento estudado (45 dias praticamente não houve diferença na brotação das mudas. Somente aquelas armazenadas por 5 (cinco semanas apresentaram inexplicavelmente um índice baixo de pegamento.

    Because of the tremendous current demand for Nanicão (Musa acuminata AAA banana shoots in the state of Goiás, notably in the “Mato Grosso Goiano” area, shoots have had to be imported from far away. The greater part of these shoots are transported in rootstalk form. Each rootstalk weighs approximately 1 Kg. A larger portion of these shoots than been anticipated, are defective. The present study was made to determine the effect of storage time on the budding of this particular banana rootstalk. The results of the experiment show that during the period of storage, forty-two (42 days, that was studied, there was almost no difference in budding among the rootstalks. Only those which were stored for five (5 weeks showed, unexplainably, a low budding index.

  13. Banana NAC transcription factor MusaNAC042 is positively associated with drought and salinity tolerance.

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    Tak, Himanshu; Negi, Sanjana; Ganapathi, T R

    2017-03-01

    Banana is an important fruit crop and its yield is hampered by multiple abiotic stress conditions encountered during its growth. The NAC (NAM, ATAF, and CUC) transcription factors are involved in plant response to biotic and abiotic stresses. In the present study, we studied the induction of banana NAC042 transcription factor in drought and high salinity conditions and its overexpression in transgenic banana to improve drought and salinity tolerance. MusaNAC042 expression was positively associated with stress conditions like salinity and drought and it encoded a nuclear localized protein. Transgenic lines of banana cultivar Rasthali overexpressing MusaNAC042 were generated by Agrobacterium-mediated transformation of banana embryogenic cells and T-DNA insertion was confirmed by PCR and Southern blot analysis. Our results using leaf disc assay indicated that transgenic banana lines were able to tolerate drought and high salinity stress better than the control plants and retained higher level of total chlorophyll and lower level of MDA content (malondialdehyde). Transgenic lines analyzed for salinity (250 mM NaCl) and drought (Soil gravimetric water content 0.15) tolerance showed higher proline content, better Fv/Fm ratio, and lower levels of MDA content than control suggesting that MusaNAC042 may be involved in responses to higher salinity and drought stresses in banana. Expression of several abiotic stress-related genes like those coding for CBF/DREB, LEA, and WRKY factors was altered in transgenic lines indicating that MusaNAC042 is an efficient modulator of abiotic stress response in banana.

  14. EFEITO DO ESPAÇAMENTO E DESBASTE NA PRODUÇÃO DA BANANEIRA DO CULTIVAR “MAÇÔ (Musa sp EFFECT OF THE SPACING AND THINNING IN BANANA (Musa sp. "MAÇÃ" CULTIVAR PRODUCTION

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    Lincoln F. Zica

    2007-09-01

    Full Text Available

    O presente trabalho teve como objetivo encontrar um espaçamento mais adequado, para conduzir os bananais do cultivar “maçã”, nas condições edáficas do Estado de Goiás. A experiência foi conduzida em solos sob cerrado, no município de Goiânia. Os seis tratamentos utilizados consistiram nas combinações de 3 (três espaçamentos com dois sistemas de condução, i.e. com e sem desbaste. A análise estatística dos dados obtidos demonstrou não haver interação desbaste x espaçamento. Com base nos resultados e observações feitas durante a fase experimental, tiraram-se as seguintes conclusões: a com o aumento da densidade de touceiras por unidade de área, há um aumento tanto do piso como do número de frutos produzidos; b O uso do desbaste não altera a produtividade, nas condições do presente experimento; c com a redução da área por touceira de 36 m² para 9 m², há um aumento na produtividade, sem prejudicar o tamanho dos frutos.

    The purpose of the present work was to determine a more adequate spacing for growing bananas (Maçã in natural conditions of the state of Goiás. The experiment was carried out in “cerrado” soils in Goiânia county. The six treatments used consisted of the combination of three (3 spacings, with and without pruning. The statistical analysis of the obtained data demonstrated that there was no interaction between pruning and spacing. The results obtained and the observations made during the spacing. The results obtained and the observations made during the experimental phase led to the following conclusions: 1. with the increase in density of the groups of shoots per unit of area, there is an increase in weight as well as an increase in the amount of fruit produced. 2. within the norms of this experiment, the use of pruning doesn’t alter productivity. 3. with

  15. Banana (Musa spp.) Production Characteristics and Performance in Uganda

    NARCIS (Netherlands)

    Bagamba, F.; Burger, C.P.J.; Tushemereirwe, W.K.

    2010-01-01

    The highland cooking banana (Musa spp., AAA-EA genome) is the most important crop in the East African Great Lakes region. In Uganda, production has expanded and productivity increased in the country’s southwest and declined in the Central region where the crop has traditional roots. Analyzing crop c

  16. Characterization of Musa sp. fruits and plantain banana ripening stages according to their physicochemical attributes.

    Science.gov (United States)

    Valérie Passo Tsamo, Claudine; Andre, Christelle M; Ritter, Christian; Tomekpe, Kodjo; Ngoh Newilah, Gérard; Rogez, Hervé; Larondelle, Yvan

    2014-08-27

    This study aimed at understanding the contribution of the fruit physicochemical parameters to Musa sp. diversity and plantain ripening stages. A discriminant analysis was first performed on a collection of 35 Musa sp. cultivars, organized in six groups based on the consumption mode (dessert or cooking banana) and the genomic constitution. A principal component analysis reinforced by a logistic regression on plantain cultivars was proposed as an analytical approach to describe the plantain ripening stages. The results of the discriminant analysis showed that edible fraction, peel pH, pulp water content, and pulp total phenolics were among the most contributing attributes for the discrimination of the cultivar groups. With mean values ranging from 65.4 to 247.3 mg of gallic acid equivalents/100 g of fresh weight, the pulp total phenolics strongly differed between interspecific and monospecific cultivars within dessert and nonplantain cooking bananas. The results of the logistic regression revealed that the best models according to fitting parameters involved more than one physicochemical attribute. Interestingly, pulp and peel total phenolic contents contributed in the building up of these models.

  17. A saturated SSR/DArT linkage map of Musa acuminata addressing genome rearrangements among bananas

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    Matsumoto Takashi

    2010-04-01

    Full Text Available Abstract Background The genus Musa is a large species complex which includes cultivars at diploid and triploid levels. These sterile and vegetatively propagated cultivars are based on the A genome from Musa acuminata, exclusively for sweet bananas such as Cavendish, or associated with the B genome (Musa balbisiana in cooking bananas such as Plantain varieties. In M. acuminata cultivars, structural heterozygosity is thought to be one of the main causes of sterility, which is essential for obtaining seedless fruits but hampers breeding. Only partial genetic maps are presently available due to chromosomal rearrangements within the parents of the mapping populations. This causes large segregation distortions inducing pseudo-linkages and difficulties in ordering markers in the linkage groups. The present study aims at producing a saturated linkage map of M. acuminata, taking into account hypotheses on the structural heterozygosity of the parents. Results An F1 progeny of 180 individuals was obtained from a cross between two genetically distant accessions of M. acuminata, 'Borneo' and 'Pisang Lilin' (P. Lilin. Based on the gametic recombination of each parent, two parental maps composed of SSR and DArT markers were established. A significant proportion of the markers (21.7% deviated (p Conclusions We propose a synthetic map with 11 linkage groups containing 489 markers (167 SSRs and 322 DArTs covering 1197 cM. This first saturated map is proposed as a "reference Musa map" for further analyses. We also propose two complete parental maps with interpretations of structural rearrangements localized on the linkage groups. The structural heterozygosity in P. Lilin is hypothesized to result from a duplication likely accompanied by an inversion on another chromosome. This paper also illustrates a methodological approach, transferable to other species, to investigate the mapping of structural rearrangements and determine their consequences on marker

  18. Phyllosticta musarum Infection-Induced Defences Suppress Anthracnose Disease Caused by Colletotrichum musae in Banana Fruits cv ‘Embul’

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    C. L. Abayasekara

    2013-03-01

    Full Text Available Anthracnose development by Colletotrichum musae was observed to be significantly less in the fruits of the banana cultivar ‘Embul’ (Mysore, AAB infected with Phyllosticta musarum than in fruits without such infections. Anthracnose disease originates from quiescent C. musae infections in the immature fruit. P. musarum incites minute, scattered spots, referred to as freckles, in the superficial tissues of immature banana peel which do not expand during maturation or ripening. P. musarum does not appear to have a direct suppressive effect on C. musae as conidia of C. musae germinate on both freckled and non-freckled fruit forming quiescent infections. Our investigations have shown that P. musarum infection induced several defence responses in fruit including the accumulation of five phytoalexins, upregulation of chitinase and β-1,3-glucanase, phenylalanine ammonia lyase (PAL activity and cell wall lignification. ¹H and ¹³C NMR spectral data of one purified phytoalexin compared closely with 4′-hydroxyanigorufone. Some of the P. musarum-induced defences that retained during ripening, restrict C. musae development at the ripe stage. This paper examines the potential of P. musarum-induced defences, in the control of anthracnose, the most destructive postharvest disease in banana.

  19. Detection of fertility and crossmatch between cultivars of Musa spp to obtain commercial hybrids

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    Lianet González Díaz

    2015-04-01

    Full Text Available This work is part of an intensive Program of Genetic improvement in Musa spp. through hybridi-zations, which holds the Research Institute of Tropical Viandas (INIVIT. For this were conducted pollen fertility tests in different cultivars of bananas and plantains and it was studied a set of combinations from preselected male and female parents from National Germplasm Bank of Musa, it is conserved “ex situ” in areas of Institute, with the objective of to determine male and female fertility in some clones and the most efficient combinations to obtain new hybrids. It was obtained as a result that the highest rates of fertility were for bananas cultivars (AA ‘SH-3142’ (95.1 %, ‘SH-3362’ (94.4 %. ‘Calcuta–4’ (99.1 %, ‘Paka’ (96.5 % and ‘Pisang Jary Buaya’ (98.1 %, the latter reported by first time under our conditions as a new male parent and they were successful in obtaining hybrid most combinations made between cooking bananas and synthetic diploid or improved (‘SH- 3362’ and ‘SH-3142’. Triploid cultivar ‘FHIA-25’, to show fewer fertile grains (17.6 and produce seeds in all combinations, reaffirms its status as maternal line

  20. Wound-induced pectin methylesterases enhance banana (Musa spp. AAA) susceptibility to Fusarium oxysporum f. sp. cubense.

    Science.gov (United States)

    Ma, Li; Jiang, Shuang; Lin, Guimei; Cai, Jianghua; Ye, Xiaoxi; Chen, Houbin; Li, Minhui; Li, Huaping; Takác, Tomás; Samaj, Jozef; Xu, Chunxiang

    2013-05-01

    Recent studies suggest that plant pectin methylesterases (PMEs) are directly involved in plant defence besides their roles in plant development. However, the molecular mechanisms of PME action on pectins are not well understood. In order to understand how PMEs modify pectins during banana (Musa spp.)-Fusarium interaction, the expression and enzyme activities of PMEs in two banana cultivars, highly resistant or susceptible to Fusarium, were compared with each other. Furthermore, the spatial distribution of PMEs and their effect on pectin methylesterification of 10 individual homogalacturonan (HG) epitopes with different degrees of methylesterification (DMs) were also examined. The results showed that, before pathogen treatment, the resistant cultivar displayed higher PME activity than the susceptible cultivar, corresponding well to the lower level of pectin DM. A significant increase in PME expression and activity and a decrease in pectin DM were observed in the susceptible cultivar but not in the resistant cultivar when plants were wounded, which was necessary for successful infection. With the increase of PME in the wounded susceptible cultivar, the JIM5 antigen (low methyestrified HGs) increased. Forty-eight hours after pathogen infection, the PME activity and expression in the susceptible cultivar were higher than those in the resistant cultivar, while the DM was lower. In conclusion, the resistant and the susceptible cultivars differ significantly in their response to wounding. Increased PMEs and thereafter decreased DMs acompanied by increased low methylesterified HGs in the root vascular cylinder appear to play a key role in determination of banana susceptibility to Fusarium.

  1. Physicochemical studies on starches isolated from plantain cultivars, plantain hybrids and cooking bananas

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    Eggleston, G.; Akoni, S. (International Inst. of Tropical Agriculture, Ibadan (Nigeria)); Swennen, R. (Catholic Univ. of Leuven, Heverlee (Belgium). Lab. of Tropical Husbandry)

    1992-04-01

    Starches from mature, unripe fruit pulp of plantain cultivars (Musa supp., AAB group) representing the wide variability in Africa, tetraploid and diploid plantain hybrids and starchy cooking bananas (Musa spp., ABB group) were isolated and characterised. In general, studies revealed very compact irregularly shaped and sized granules, with low amylose content (9.11-17.16%), highly resistant to bacterial {alpha}-amylase attack; Brabender amylograms showed very restricted swelling type patterns with great stability and negligible retrogradation. Results indicate that differences in physico-chemical properties exist amongst the three Musa fruit group starches. Plantains represent a chemical/molecular homogeneous group, but heterogeneous for granule structure. Ploidy level affected hybrid properties. ABB cooking bananas starches exhibited highly pronounced restricted swelling and high gelatinisation and pasting temperatures, indicating a more ordered, very strongly bonded granule structure; chemical and physical properties varied considerably within the ABB genotype. (orig.).

  2. The natural impact of banana inflorescences (Musa acuminata on human nutrition

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    Catharina E. Fingolo

    2012-12-01

    Full Text Available Banana inflorescences are popularly known as 'navels,' and they are used in Brazil as nutritional complements. However, the nutritional value of banana inflorescences (male flowers and bracts has never been studied. Therefore, plant material of Musa acuminata, cultivar "ouro", was collected in Rio de Janeiro state, Brazil, and then submitted to chemical procedures to determine its nutritional composition. The experiment was arranged a completely randomized design and performed in triplicate. The sample composition analysis showed percentual average value for moisture, protein, fat and ash as 8.21, 14.50, 4.04 and 14.43, respectively. The dehydrated inflorescences were found to contain a significant nutritive complement based on their high content of potassium (5008.26 mg / 100 g and fiber 49.83% (lignin, cellulose and hemicelluloses revealing important functional and nutritional properties. In a parallel evaluation, the anatomical study revealed key elements for the recognition of Musa acuminata when reduced to fragments.Inflorescências de bananeira são popularmente conhecidas como "umbigos", e esses são usados no Brasil como complementos nutricionais. No entanto, o valor nutricional das inflorescências (flores masculinas e brácteas de bananeira nunca foi estudado. Portanto, o material vegetal de Musa acuminata cultivar "ouro" foi coletado no Rio de Janeiro, Brasil e, em seguida, submetido aos procedimentos químicos para determinar a sua composição nutricional. As análises foram realizadas em triplicata. A análise da composição da amostra apresentou teor percentual médio de umidade, proteína, lipídeos e cinza de 8,21, 14,50, 4,04 e 14,43, respectivamente. As inflorescências secas mostraram significativo complemento nutritivo baseado no alto conteúdo de potássio (5.008,26 mg / 100 g e de fibra 49,83% (lignina, celulose e hemiceluloses revelando importantes propriedades funcional e nutritiva. Em uma avaliação paralela, o estudo

  3. Activation of salicylic acid metabolism and signal transduction can enhance resistance to Fusarium wilt in banana (Musa acuminata L. AAA group, cv. Cavendish).

    Science.gov (United States)

    Wang, Zhuo; Jia, Caihong; Li, Jingyang; Huang, Suzhen; Xu, Biyu; Jin, Zhiqiang

    2015-01-01

    Fusarium wilt caused by the fungus Fusarium oxysporum f. sp. cubens (Foc) is the most serious disease that attacks banana plants. Salicylic acid (SA) can play a key role in plant-microbe interactions. Our study is the first to examine the role of SA in conferring resistance to Foc TR4 in banana (Musa acuminata L. AAA group, cv. Cavendish), which is the greatest commercial importance cultivar in Musa. We used quantitative real-time reverse polymerase chain reaction (qRT-PCR) to analyze the expression profiles of 45 genes related to SA biosynthesis and downstream signaling pathways in a susceptible banana cultivar (cv. Cavendish) and a resistant banana cultivar (cv. Nongke No. 1) inoculated with Foc TR4. The expression of genes involved in SA biosynthesis and downstream signaling pathways was suppressed in a susceptible cultivar and activated in a resistant cultivar. The SA levels in each treatment arm were measured using high-performance liquid chromatography. SA levels were decreased in the susceptible cultivar and increased in the resistant cultivar. Finally, we examined the contribution of exogenous SA to Foc TR4 resistance in susceptible banana plants. The expression of genes involved in SA biosynthesis and signal transduction pathways as well as SA levels were significantly increased. The results suggest that one reason for banana susceptibility to Foc TR4 is that expression of genes involved in SA biosynthesis and SA levels are suppressed and that the induced resistance observed in banana against Foc TR4 might be a case of salicylic acid-dependent systemic acquired resistance.

  4. “EFEITO DO PESO DA MUDA TIPO PEDAÇO DE RIZOMA, NO ENRAIZAMENTO INICIAL DA BANANEIRA DO CULTIVAR MARMELO (Musa sp ABB” EFFECT OF THE WEIGHT OF RHIZOME TYPE SEEDLING IN THE INITIAL ROOTING OF BANANA CULTIVAR MARMELO (Musa sp. ABB

    Directory of Open Access Journals (Sweden)

    Raimundo Ney de Macedo Lima

    2007-09-01

    Full Text Available

    Um dos maiores problemas na formação de um bananal é a obtenção de mudas sadias e em quantidade suficiente nas proximidades da lavoura. O custo do transporte deste material, assim como o preço é muito alto. Uma solução para amenizar o problema é a utilização de pedaços de rizoma. O presente trabalho foi realizado com finalidade de determinar o tamanho mínimo do pedaço de rizoma que poderá ser recomendado quando se utiliza o cultivar “marmelo” sob nossas condições. Com base nos resultados conclui-se que: a - Quanto maior o pedaço de rizoma, maior o enraizamento; b - O peso mínimo a ser utilizado diretamente no campo é de 750 g; c - Os pedaços de 250 g apresentam bom índice de brotação e podem ser utilizados para propagação rápida.

    One of the greatest problems in the formation of a banana plantation is obtaining healthy shoots in sufficient quantity near the chosen site. The cost of transportation, along with the price of the shoots, is very high. One solution for this problem is to use bits. This study was undertaken to determine the smallest piece of rizoma which could de used in the cultivation of the banana “marmelo” under our existing conditions.

  5. Banana infecting fungus, Fusarium musae, is also an opportunistic human pathogen: are bananas potential carriers and source of fusariosis?

    Science.gov (United States)

    Triest, David; Stubbe, Dirk; De Cremer, Koen; Piérard, Denis; Detandt, Monique; Hendrickx, Marijke

    2015-01-01

    During re-identification of Fusarium strains in the BCCM™/IHEM fungal collection by multilocus sequence-analysis we observed that five strains, previously identified as Fusarium verticillioides, were Fusarium musae, a species described in 2011 from banana fruits. Four strains were isolated from blood samples or biopsies of immune-suppressed patients and one was isolated from the clinical environment, all originating from different hospitals in Belgium or France, 2001-2008. The F. musae identity of our isolates was confirmed by phylogenetic analysis using reference sequences of type material. Absence of the gene cluster necessary for fumonisin biosynthesis, characteristic to F. musae, was also the case for our isolates. In vitro antifungal susceptibility testing revealed no important differences in their susceptibility compared to clinical F. verticillioides strains and terbinafine was the most effective drug. Additional clinical F. musae strains were searched by performing BLAST queries in GenBank. Eight strains were found, of which six were keratitis cases from the U.S. multistate contact lens-associated outbreak in 2005 and 2006. The two other strains were also from the U.S., causing either a skin infection or sinusitis. This report is the first to describe F. musae as causative agent of superficial and opportunistic, disseminated infections in humans. Imported bananas might act as carriers of F. musae spores and be a potential source of infection with F. musae in humans. An alternative hypothesis is that the natural distribution of F. musae is geographically a lot broader than originally suspected and F. musae is present on different plant hosts.

  6. Occurrence of Fusarium verticillioides and Fusarium musae on banana fruits marketed in Hungary.

    Science.gov (United States)

    Molnár, Orsolya; Bartók, Tibor; Szécsi, Árpád

    2015-06-01

    Fusarium strains were isolated from rotten banana fruit imported into Hungary from some African and some Neotropical countries. The strains were identified using morphological features, 2-benzoxazolinone tolerance, translation elongation factor (EF-1α) sequences and inter simple sequence repeat (ISSR) analysis. All strains from Africa proved to be F. verticillioides whereas the strains from the Neotropics are Fusarium musae. According to the PCR proof and the fumonisin toxin measurement F. musae strains cannot produce any fumonisins (FB1-4).

  7. Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers

    Directory of Open Access Journals (Sweden)

    YUYU SURYASARI POERBA

    2010-07-01

    Full Text Available Poerba YS, Ahmad F (2010 Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers. Biodiversitas 11: 118-123. This study was done to assess the molecular diversity of 36 accessions (18 cultivars of the plantain and cooking bananas (Musa acuminata x M. balbisiana, AAB, ABB subgroups based on Random amplified polymorphic DNA (RAPD and and Inter Simple Sequence Repeats (ISSR markers and to determine genetic relationships in the bananas. RAPD and ISSR fingerprinting of these banana varieties was carried out by five primers of RAPDs and two primers of ISSRs. RAPD primers produced 63 amplified fragments varying from 250 to 2500 bp in size. 96.82% of the amplification bands were polymorphic. ISSR primers produced 26 amplified fragments varying from 350 bp to 2000 bp in size. The results showed that 92.86% of the amplification bands were polymorphic. The range of genetic distance of 18 cultivars was from 0.06-0.67.

  8. Evaluation and characterization in bananas (Musa ssp.) at the USDA-ARS Tropical Agriculture Research Station

    Science.gov (United States)

    Banana, Musa spp., is a key horticultural crop in tropical regions of the world where they provide sustenance and serve as cash crops. The plantain subgroup in particular, is an important staple in the Caribbean, Central America and some countries in South America. One of the integral research comp...

  9. Consumer Perceptions towards Introducing a Genetically Modified Banana (Musa spp.) in Uganda

    NARCIS (Netherlands)

    Kikulwe, E.M.; Wesseler, J.H.H.; Falck-Zepeda, J.

    2010-01-01

    The introduction of a genetically modified (GM) banana (Musa spp.) in Uganda is not without controversy. It is likely to generate a wide portfolio of concerns as the technology of genetic engineering is still in its early stages of development in Uganda. The purpose of this study is to show how cons

  10. The banana (Musa acuminata) genome and the evolution of monocotyledonous plants.

    Science.gov (United States)

    D'Hont, Angélique; Denoeud, France; Aury, Jean-Marc; Baurens, Franc-Christophe; Carreel, Françoise; Garsmeur, Olivier; Noel, Benjamin; Bocs, Stéphanie; Droc, Gaëtan; Rouard, Mathieu; Da Silva, Corinne; Jabbari, Kamel; Cardi, Céline; Poulain, Julie; Souquet, Marlène; Labadie, Karine; Jourda, Cyril; Lengellé, Juliette; Rodier-Goud, Marguerite; Alberti, Adriana; Bernard, Maria; Correa, Margot; Ayyampalayam, Saravanaraj; Mckain, Michael R; Leebens-Mack, Jim; Burgess, Diane; Freeling, Mike; Mbéguié-A-Mbéguié, Didier; Chabannes, Matthieu; Wicker, Thomas; Panaud, Olivier; Barbosa, Jose; Hribova, Eva; Heslop-Harrison, Pat; Habas, Rémy; Rivallan, Ronan; Francois, Philippe; Poiron, Claire; Kilian, Andrzej; Burthia, Dheema; Jenny, Christophe; Bakry, Frédéric; Brown, Spencer; Guignon, Valentin; Kema, Gert; Dita, Miguel; Waalwijk, Cees; Joseph, Steeve; Dievart, Anne; Jaillon, Olivier; Leclercq, Julie; Argout, Xavier; Lyons, Eric; Almeida, Ana; Jeridi, Mouna; Dolezel, Jaroslav; Roux, Nicolas; Risterucci, Ange-Marie; Weissenbach, Jean; Ruiz, Manuel; Glaszmann, Jean-Christophe; Quétier, Francis; Yahiaoui, Nabila; Wincker, Patrick

    2012-08-01

    Bananas (Musa spp.), including dessert and cooking types, are giant perennial monocotyledonous herbs of the order Zingiberales, a sister group to the well-studied Poales, which include cereals. Bananas are vital for food security in many tropical and subtropical countries and the most popular fruit in industrialized countries. The Musa domestication process started some 7,000 years ago in Southeast Asia. It involved hybridizations between diverse species and subspecies, fostered by human migrations, and selection of diploid and triploid seedless, parthenocarpic hybrids thereafter widely dispersed by vegetative propagation. Half of the current production relies on somaclones derived from a single triploid genotype (Cavendish). Pests and diseases have gradually become adapted, representing an imminent danger for global banana production. Here we describe the draft sequence of the 523-megabase genome of a Musa acuminata doubled-haploid genotype, providing a crucial stepping-stone for genetic improvement of banana. We detected three rounds of whole-genome duplications in the Musa lineage, independently of those previously described in the Poales lineage and the one we detected in the Arecales lineage. This first monocotyledon high-continuity whole-genome sequence reported outside Poales represents an essential bridge for comparative genome analysis in plants. As such, it clarifies commelinid-monocotyledon phylogenetic relationships, reveals Poaceae-specific features and has led to the discovery of conserved non-coding sequences predating monocotyledon-eudicotyledon divergence.

  11. Effect of surface coating on ripening and early peel spotting in 'Sucrier' banana (Musa acuminata)

    NARCIS (Netherlands)

    Promyou, S.; Ketsa, S.; Doorn, van W.G.

    2007-01-01

    Sucrier¿ bananas (Musa acuminata, AA Group) show peel spotting when the peel is just about as yellow as green, which coincides with optimum eating quality. As consumers might relate the spotting to overripe fruit, early spotting is considered undesirable, especially for export markets. Fruit were le

  12. Catalog of banana (Musa spp.) accessions maintained at the USDA-ARS, Tropical Agriculture Reserach Station

    Science.gov (United States)

    Banana genetic resources can be found in situ in native habitats in Southeast Asia and the Pacific region. Ex situ collections also exist in important tropical regions of the world as well as in vitro cultures at the Bioversity International Musa Germplasm Transit Centre. Unfortunately, readily avai...

  13. Structures of chlorophyll catabolites in bananas (Musa acuminata) reveal a split path of chlorophyll breakdown in a ripening fruit.

    Science.gov (United States)

    Moser, Simone; Müller, Thomas; Holzinger, Andreas; Lütz, Cornelius; Kräutler, Bernhard

    2012-08-27

    The disappearance of chlorophyll is a visual sign of fruit ripening. Yet, chlorophyll breakdown in fruit has hardly been explored; its non-green degradation products are largely unknown. Here we report the analysis and structure elucidation of colorless tetrapyrrolic chlorophyll breakdown products in commercially available, ripening bananas (Musa acuminata, Cavendish cultivar). In banana peels, chlorophyll catabolites were found in an unprecedented structural richness: a variety of new fluorescent chlorophyll catabolites (FCCs) and nonfluorescent chlorophyll catabolites (NCCs) were detected. As a rule, FCCs exist only "fleetingly" and are hard to observe. However, in bananas several of the FCCs (named Mc-FCCs) were persistent and carried an ester function at the propionate side-chain. NCCs were less abundant, and exhibited a free propionic acid group, but functional modifications elsewhere. The modifications of NCCs in banana peels were similar to those found in NCCs from senescent leaves. They are presumed to be introduced by enzymatic transformations at the stage of the mostly unobserved, direct FCC-precursors. The observed divergent functional group characteristics of the Mc-FCCs versus those of the Mc-NCCs indicated two major "late" processing lines of chlorophyll breakdown in ripening bananas. The "last common precursor" at the branching point to either the persistent FCCs, or towards the NCCs, was identified as a temporarily abundant "secondary" FCC. The existence of two "downstream" branches of chlorophyll breakdown in banana peels, and the striking accumulation of persistent Mc-FCCs call for attention as to the still-elusive biological roles of the resulting colorless linear tetrapyrroles.

  14. Structures of Chlorophyll Catabolites in Bananas (Musa acuminata) Reveal a Split Path of Chlorophyll Breakdown in a Ripening Fruit

    Science.gov (United States)

    Moser, Simone; Müller, Thomas; Holzinger, Andreas; Lütz, Cornelius; Kräutler, Bernhard

    2012-01-01

    Abstract The disappearance of chlorophyll is a visual sign of fruit ripening. Yet, chlorophyll breakdown in fruit has hardly been explored; its non-green degradation products are largely unknown. Here we report the analysis and structure elucidation of colorless tetrapyrrolic chlorophyll breakdown products in commercially available, ripening bananas (Musa acuminata, Cavendish cultivar). In banana peels, chlorophyll catabolites were found in an unprecedented structural richness: a variety of new fluorescent chlorophyll catabolites (FCCs) and nonfluorescent chlorophyll catabolites (NCCs) were detected. As a rule, FCCs exist only "fleetingly" and are hard to observe. However, in bananas several of the FCCs (named Mc-FCCs) were persistent and carried an ester function at the propionate side-chain. NCCs were less abundant, and exhibited a free propionic acid group, but functional modifications elsewhere. The modifications of NCCs in banana peels were similar to those found in NCCs from senescent leaves. They are presumed to be introduced by enzymatic transformations at the stage of the mostly unobserved, direct FCC-precursors. The observed divergent functional group characteristics of the Mc-FCCs versus those of the Mc-NCCs indicated two major "late" processing lines of chlorophyll breakdown in ripening bananas. The "last common precursor" at the branching point to either the persistent FCCs, or towards the NCCs, was identified as a temporarily abundant "secondary" FCC. The existence of two "downstream" branches of chlorophyll breakdown in banana peels, and the striking accumulation of persistent Mc-FCCs call for attention as to the still-elusive biological roles of the resulting colorless linear tetrapyrroles. PMID:22807397

  15. Development of Green Banana (Musa paradisiaca as Potential Food Packaging Films and Coatings

    Directory of Open Access Journals (Sweden)

    Nur Hanani Z. A.

    2016-02-01

    Full Text Available The aim of this study was to develop biodegradable packaging films based on a unripe green banana (Musa paradisiaca L. with different plasticizers; glycerol, polyethylene glycol (PEG and sorbitol at various concentrations (10-50%. Banana films were produced by using casting method and physical properties of these films were determined. Banana films with 10% of PEG showed the lowest water solubility (P≤0.05 followed by films with glycerol and sorbitol. Banana films with 40% plasticizers possessed the lowest water vapor permeability (WVP whereas films with 30% glycerol exhibited higher values of tensile strength (P≤0.05 compared to films with PEG and sorbitol. However, types of plasticizers did not influence the thickness of the films. Also, used of higher concentrations of plasticizers had increased the solubility values. These findings reveal that concentrations and types of plasticizers have significant roles to provide banana film or coating with good physical properties. The aim of this study was to develop biodegradable packaging films based on a unripe green banana (Musa paradisiaca L. with different plasticizers; glycerol, polyethylene glycol (PEG and sorbitol at various concentrations (10-50%. Banana films were produced by using casting method and physical properties of these films were determined. Banana films with 10% of PEG showed the lowest water solubility (P≤0.05 followed by films with glycerol and sorbitol. Banana films with 40% plasticizers possessed the lowest water vapour permeability (WVP whereas films with 30% glycerol exhibited higher values of tensile strength (P≤0.05 compared to films with PEG and sorbitol. However, types of plasticizers did not influence the thickness of the films. Also, used of higher concentrations of plasticizers had increased the solubility values. These findings reveal that concentrations and types of plasticizers have significant roles to provide banana film or coating with good physical

  16. Socioeconomic importance of the banana tree (Musa spp.) in the Guinean Highland Savannah agroforests.

    Science.gov (United States)

    Mapongmetsem, Pierre Marie; Nkongmeneck, Bernard Aloys; Gubbuk, Hamide

    2012-01-01

    Home gardens are defined as less complex agroforests which look like and function as natural forest ecosystems but are integrated into agricultural management systems located around houses. Investigations were carried out in 187 households. The aim of the study was to identify the different types of banana home gardens existing in the periurban zone of Ngaoundere town. The results showed that the majority of home gardens in the area were very young (less than 15 years old) and very small in size (less than 1 ha). Eleven types of home gardens were found in the periurban area of Ngaoundere town. The different home garden types showed important variations in all their structural characteristics. Two local species of banana are cultivated in the systems, Musa sinensis and Musa paradisiaca. The total banana production is 3.57 tons per year. The total quantity of banana consumed in the periurban zone was 3.54 tons (93.5%) whereas 1.01 tons were sold in local or urban markets. The main banana producers belonged to home gardens 2, 4, 7, and 9. The quantity of banana offered to relatives was more than what the farmers received from others. Farmers, rely on agroforests because the flow of their products helps them consolidate friendship and conserve biodiversity at the same time.

  17. Socioeconomic Importance of the Banana Tree (Musa Spp. in the Guinean Highland Savannah Agroforests

    Directory of Open Access Journals (Sweden)

    Pierre Marie Mapongmetsem

    2012-01-01

    Full Text Available Home gardens are defined as less complex agroforests which look like and function as natural forest ecosystems but are integrated into agricultural management systems located around houses. Investigations were carried out in 187 households. The aim of the study was to identify the different types of banana home gardens existing in the periurban zone of Ngaoundere town. The results showed that the majority of home gardens in the area were very young (less than 15 years old and very small in size (less than 1 ha. Eleven types of home gardens were found in the periurban area of Ngaoundere town. The different home garden types showed important variations in all their structural characteristics. Two local species of banana are cultivated in the systems, Musa sinensis and Musa paradisiaca. The total banana production is 3.57 tons per year. The total quantity of banana consumed in the periurban zone was 3.54 tons (93.5% whereas 1.01 tons were sold in local or urban markets. The main banana producers belonged to home gardens 2, 4, 7, and 9. The quantity of banana offered to relatives was more than what the farmers received from others. Farmers, rely on agroforests because the flow of their products helps them consolidate friendship and conserve biodiversity at the same time.

  18. Expression and distribution of extensins and AGPs in susceptible and resistant banana cultivars in response to wounding and Fusarium oxysporum

    Science.gov (United States)

    Wu, Yunli; Fan, Wei; Li, Xiaoquan; Chen, Houbin; Takáč, Tomáš; Šamajová, Olga; Fabrice, Musana Rwalinda; Xie, Ling; Ma, Juan; Šamaj, Jozef; Xu, Chunxiang

    2017-01-01

    Banana Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc) is soil-borne disease of banana (Musa spp.) causing significant economic losses. Extensins and arabinogalactan proteins (AGPs) are cell wall components important for pathogen defence. Their significance for Foc resistance in banana was not reported so far. In this study, two banana cultivars differing in Foc sensitivity were used to monitor the changes in transcript levels, abundance and distribution of extensins and AGPs after wounding and Foc inoculation. Extensins mainly appeared in the root cap and meristematic cells. AGPs recognized by JIM13, JIM8, PN16.4B4 and CCRC-M134 antibodies located in root hairs, xylem and root cap. Individual AGPs and extensins showed specific radial distribution in banana roots. At the transcript level, seven extensins and 23 AGPs were differentially expressed between two banana cultivars before and after treatments. Two extensins and five AGPs responded to the treatments at the protein level. Most extensins and AGPs were up-regulated by wounding and pathogen inoculation of intact plants but down-regulated by pathogen attack of wounded plants. Main components responsible for the resistance of banana were MaELP-2 and MaPELP-2. Our data revealed that AGPs and extensins represent dynamic cell wall components involved in wounding and Foc resistance. PMID:28218299

  19. Eficiência hospedeira de oito cultivares de bananeira a fitonematóides Host reaction of eight banana cultivars to plant parasitic nematodes

    Directory of Open Access Journals (Sweden)

    Leila Lucí Dinardo-Miranda

    1996-01-01

    Full Text Available Os cultivares de bananeira (Musa spp. Grande Naine, Nanicão Jangada, Nanicão (Grupo AAA, Enxerto, Prata, Prata Zulu, Maçã e Mysore (Grupo AAB foram plantados em campo e avaliados quanto à eficiência hospedeira a nematóides. Todos eles apresentaram elevadas populações de Meloidogyne arenaria raça 1, sendo considerados, portanto, hospedeiros favoráveis dessa espécie. Os cultivares do grupo AAA e Mysore foram também hospedeiros favoráveis de Radopholus similis, enquanto os demais do grupo AAB se comportaram como hospedeiros pouco favoráveis. Helicotylenchus dihystera foi detectado em baixas populações, em raízes e no solo da rizosfera de todos os cultivares.The host reaction of eight banana cultivars (Musa spp. to nematodes was assessed in a field experiment. Three cultivars evaluated were members of AAA Group (Grande Naine, Nanicão and Nanicão Jangada and five belonged to AAB Group (Prata, Enxerto, Prata Zulu, Maçã and Mysore. All cultivars were infested by Meloidogyne arenaria race 1, rated as efficient hosts. The AAA Group cultivars and Mysore were also efficient hosts to Radopholus similis, whereas Prata, Enxerto and Maçã were not. Helicotylenchus dihystera was detected at low density populations in soil and root samples of all cultivars.

  20. Phenylphenalenones Accumulate in Plant Tissues of Two Banana Cultivars in Response to Herbivory by the Banana Weevil and Banana Stem Weevil

    Science.gov (United States)

    Hölscher, Dirk; Buerkert, Andreas; Schneider, Bernd

    2016-01-01

    Phenylphenalenone-type compounds accumulated in the tissues of two banana cultivars—Musa acuminata cv. “Grande Naine” (AAA) and Musa acuminata × balbisiana Colla cv. “Bluggoe” (ABB)—when these were fed on by the banana weevil (Cosmopolites sordidus (Germ.) (Coleoptera: Curculionidae)) and the banana stem weevil (Odoiporus longicollis (Oliver) (Coleoptera: Curculionidae)). The chemical constituents of the banana material were separated by means of chromatographic techniques and identified by NMR spectroscopy. One new compound, 2-methoxy-4-phenylphenalen-1-one, was found exclusively in the corm material of “Bluggoe” that had been fed on by the weevils. PMID:27571112

  1. Insights into the Musa genome: Syntenic relationships to rice and between Musa species

    NARCIS (Netherlands)

    Piffanelli, P.; Ciampi, A.Y.; Silva, F.R.; Santos, C.R.; Dhont, A.; Vilarinhos, A.; Pappas, G.; Souza, M.T.; Milller, R.N.G.

    2008-01-01

    Musa species (Zingiberaceae, Zingiberales) including bananas and plantains are collectively the fourth most important crop in developing countries. Knowledge concerning Musa genome structure and the origin of distinct cultivars has greatly increased over the last few years. Until now, however, no la

  2. Maternal inheritance of chloroplast genome and paternal inheritance of mitochondrial genome in bananas (Musa acuminata).

    Science.gov (United States)

    Fauré, S; Noyer, J L; Carreel, F; Horry, J P; Bakry, F; Lanaud, C

    1994-03-01

    Restriction fragment length polymorphisms (RFLPs) were used as markers to determine the transmission of cytoplasmic DNA in diploid banana crosses. Progenies from two controlled crosses were studied with heterologous cytoplasmic probes. This analysis provided evidence for a strong bias towards maternal transmission of chloroplast DNA and paternal transmission of mitochondrial DNA in Musa acuminata. These results suggest the existence of two separate mechanisms of organelle transmission and selection, but no model to explain this can be proposed at the present time. Knowledge of the organelle mode of inheritance constitutes an important point for phylogeny analyses in bananas and may offer a powerful tool to confirm hybrid origins.

  3. Characterization of some physicist, mechanics and chemistries properties in the banana (Musa spp.)

    OpenAIRE

    Carlos Modesto Martínez Hernández; Tania de la Caridad Bermúdez Camacho

    2016-01-01

    The present work approaches the study of some physical-mechanical and chemical properties of the banana (Musa spp.). For its investigation, they took samples in the Municipal Company of Cultivos Varios, municipality Taguasco, provinces of Sancti-Spíritus. They were carried out rehearsals related to some physical, mechanical and chemical variables as: pH, oBrix, total regular acids, static coefficients of friction and mechanical impact damages deal with the established norms. The objective of ...

  4. Production of haploids from anther culture of banana [Musa balbisiana (BB)].

    Science.gov (United States)

    Assani, A; Bakry, F; Kerbellec, F; Haïcour, R; Wenzel, G; Foroughi-Wehr, B

    2003-02-01

    We report here, for the first time, the production of haploid plants of banana Musa balbisiana (BB). Callus was induced from anthers in which the majority of the microspores were at the uninucleate stage. The frequency of callus induction was 77%. Callus proliferation usually preceded embryo formation. About 8% of the anthers developed androgenic embryos. Of the 147 plantlets obtained, 41 were haploids (n=x=11). The frequency of haploid production depended on genotypes used: 18 haploid plants were produced from genotype Pisang klutuk, 12 from Pisang batu, seven from Pisang klutuk wulung and four from Tani. The frequency of regeneration was 1.1%, which was based on the total number of anthers cultured. Diploid plants (2n=2x=22) were also observed in the regenerated plants. The haploid banana plants that were developed will be important material for the improvement of banana through breeding programmes.

  5. EFEITO DO PESO DE MUDA TIPO PEDAÇO DE RIZOMA NO ENRAIZAMENTO INICIAL DA BANANEIRA DO CULTIVAR NANICÃO (Musa acuminata AAA EFFECT OF THE WEIGHT OF RHIZOME TYPE SEEDLING IN THE INITIAL ROOTING OF BANANA CULTIVAR NANICÃO (Musa acuminata AAA

    Directory of Open Access Journals (Sweden)

    Lincoln F. Zica

    2007-09-01

    Full Text Available

    O uso de mudas do tipo pedaço de rizoma, vem, a cada dia, sendo procurado intensivamente como material de propagação de bananeiras. Porém, ao se trabalhar com esse tipo de mudas há necessidades de se conhecer o efeito do peso do rizoma em relação ao seu enraizamento inicial, pois quanto maior o peso das raízes, melhores serão as mudas. De acordo com a análise estatística concluiu-se que há um maior enraizamento em pedaços de rizomas de maior peso.

    Pieces of rootstalk are being used more and more intensively as a means of reproducing banana plants. However, in order to work effectively with this means of reproduction, it is necessary to know the effect of the rootstalk’s weight when it is first planted, because the greater the weight of the rootstalk, the better the shoots will be. On the basis of statistical analysis, it was concluded that the heavier pieces of rootstalk root better.

  6. Construction and characterization of a plant transformation-competent BIBAC library of the black Sigatoka-resistant banana Musa acuminata cv. Tuu Gia (AA).

    Science.gov (United States)

    Ortiz-Vázquez, E; Kaemmer, D; Zhang, H-B; Muth, J; Rodríguez-Mendiola, M; Arias-Castro, C; James, Andrew

    2005-02-01

    A plant transformation-competent binary bacterial artificial chromosome (BIBAC) library was constructed from Musa acuminata cv. Tuu Gia (AA), a black Sigatoka-resistant diploid banana. After digestion of high-molecular-weight banana DNA by HindIII, several methods of DNA size selection were tested, followed by ligation, using a vector/insert molar ratio of 4:1. The library consists of 30,700 clones stored in 80 384-well microtiter plates. The mean insert size was estimated to be 100 kb, and the frequency of inserts with internal NotI sites was 61%. The majority of insert sizes fell into the range of 100+/-20 kb, making them suitable for Agrobacterium-mediated transformation. Only 1% and 0.9% of the clones contain chloroplast and mitochondrial DNA, respectively. This is the first BIBAC library for banana, estimated to represent five times its haploid genome (600 Mbp). It was demonstrated by hybridization that the library contains typical members of resistance gene and defense gene families that can be used for transformation of disease susceptible banana cultivars for banana genetic improvement.

  7. Wound healing activity of methanolic stem extract of Musa paradisiaca Linn. (Banana) in Wistar albino rats.

    Science.gov (United States)

    Amutha, Kuppusamy; Selvakumari, Ulagesan

    2016-10-01

    This study is designed to explore the phytochemical, antibacterial and wound healing activity of methanolic stem extract of Musa paradisiaca Linn. (Banana). The phytochemical analysis was performed for the methanolic stem extract of Musa paradisiaca Linn. Results indicates that the Musa paradisiaca Linn. was rich in glucosides, tannins and alkaloids, saponins, flavonoids and phenols were present in moderate quantities. The extract shows antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus with the zone of inhibition of Pseudomonas aeruginosa was 21 mm and Staphylococcus aureus was 19 mm at concentration of 500 µg/disc. The minimum inhibitory concentration (MIC) was also evaluated for the extract. Wistar albino rats were selected for wound healing activity. The burn wound was created by using red hot steel rod from above the hind limb region. The methanolic extract was applied on the wound and the progressive changes were monitored every day. The wound contraction rate was absorbed based on the histopathological examination. It was concluded that the methanolic extract of Musa paradisiaca Linn. showed greater healing activity compared to control in Wistar albino rats.

  8. EXTRACTION AND QUANTITATIVE DETERMINATION OF ASCORBIC ACID FROM BANANA PEEL MUSA ACUMINATA ‘KEPOK’

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    Khairul Anwar Mohamad Said

    2016-04-01

    Full Text Available This paper discusses the extraction of an antioxidant compound, which is ascorbic acid or vitamin C, from a banana peel using an ultrasound-assisted extraction (UAE method. The type of banana used was Musa acuminata also known as “PisangKepok” in Malaysia. The investigation includes the effect of solvent/solid ratio (4.5, 5 g and 10  ml/g, sonication time (15, 30 and 45 mins and temperature variation (30 , 45  and 60oC on the extraction of ascorbic acid compounds from the banana peel to determine the best or optimum condition of the operation. Out of all extract samples analyzed by redox titration method using iodine solution, it was found that the highest yield was 0.04939 ± 0.00080 mg that resulted from an extraction at 30oC for 15 mins with 5 ml/g solvent-to-solute ratio.KEYWORDS:  Musa acuminata; ultrasound-assisted extraction; vitamin C; redox titration

  9. Whole genome sequencing of a banana wild relative Musa itinerans provides insights into lineage-specific diversification of the Musa genus.

    Science.gov (United States)

    Wu, Wei; Yang, Yu-Lan; He, Wei-Ming; Rouard, Mathieu; Li, Wei-Ming; Xu, Meng; Roux, Nicolas; Ge, Xue-Jun

    2016-08-17

    Crop wild relatives are valuable resources for future genetic improvement. Here, we report the de novo genome assembly of Musa itinerans, a disease-resistant wild banana relative in subtropical China. The assembled genome size was 462.1 Mb, covering 75.2% of the genome (615.2Mb) and containing 32, 456 predicted protein-coding genes. Since the approximate divergence around 5.8 million years ago, the genomes of Musa itinerans and Musa acuminata have shown conserved collinearity. Gene family expansions and contractions enrichment analysis revealed that some pathways were associated with phenotypic or physiological innovations. These include a transition from wood to herbaceous in the ancestral Musaceae, intensification of cold and drought tolerances, and reduced diseases resistance genes for subtropical marginally distributed Musa species. Prevalent purifying selection and transposed duplications were found to facilitate the diversification of NBS-encoding gene families for two Musa species. The population genome history analysis of M. itinerans revealed that the fluctuated population sizes were caused by the Pleistocene climate oscillations, and that the formation of Qiongzhou Strait might facilitate the population downsizing on the isolated Hainan Island about 10.3 Kya. The qualified assembly of the M. itinerans genome provides deep insights into the lineage-specific diversification and also valuable resources for future banana breeding.

  10. Antioxidant activity test on ambonese banana stem sap (Musa parasidiaca var. sapientum

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    Hendrik Setia Budi

    2015-12-01

    Full Text Available Background: Polymorphonuclear cells (PMN release oxygen free radicals or reactive oxygen species (ROS during inflammation. As a result, ROS level is higher than antioxidant level in our body during oxidative stress leading to prolong inflammation or continuous tissue damage. Indonesia, on the other hand, is a country with various herbal medicines. For instance, ambonese banana (Musa parasidiaca var. sapientum is often used as herbal medicine. Ambonese banana, moreover, has flavonoid, polyphenol, tannin, and saponin as antioxidants to reduce free radicals by transferring their hydrogen atom. Medicine used to reduce the impact of free radicals is known as antioxidant. Antioxidant is proved to accelerate wound healing. Purpose: This research aims to analyze the effects of the antioxidant activity of Ambonese banana stem sap extract. Method: Antioxidant activities in this research were examined with 1,1-Diphenyl-2-picryl-hidrazyl (DPPH method by reacting with stable radical compounds. Spectrophotometry with a wavelength of 517 nm was used to measure absorption results shown in purple. The absorption results then were calculated by IC50 reduction activity. Result: There were significant differences of Ambonese banana stem sap antioxidant activity (p50%. Conclusion: Ambonese banana stem sap extract has antioxidant activities.

  11. CARCASS YIELD OF BROILER CHICKENS FED BANANA (Musa paradisiaca LEAVES FERMENTED WITH Trichoderma viride

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    J. S. Mandey

    2016-03-01

    Full Text Available The study was conducted to evaluate the effect of level of banana (Musa paradisiaca leaves fermented with Trichoderma viride at different days on the carcass yield of broiler chickens. A hundred and eighty 3-weeks-old broiler chicks were used in this present experiment based on factorial design (3×4. The birds were randomly allocated into three experimental diets containing of 5, 10 and 15% of banana leaves fermented within 0, 5, 10 and 15 days. Each treatment was divided into three replicates of five chicks in each. The experiment was terminated after 4 weeks or when the birds were 7-weeks-old. Feed intake, body weight gain, feed efficiency and carcass yield were measured during the study. The data were subjected to the analysis of variance test followed by least significant difference (LSD test. Results showed that daily feed intake was significantly affected (P˂0.01 by the dietary treatments, in which feed intake was highest in broilers fed diet containing 10% banana leaves fermented for 10 days. The daily weight gain, feed efficiency and carcass yield were significantly affected (P˂0.01 by the treatments, in which the highest values of daily weight gain, feed efficiency, and carcass yield were observed in birds fed diet containing 10% banana leaves fermented for 10 days. It can be concluded that diet containing 10% banana leaves fermented for 10 days can be included in broiler ration without detrimental effects on the performance and carcass yield. 

  12. Caracterização do grânulo de amido de bananas (Musa AAA-Nanicão e Musa AAB-Terra Characterization of starch granules from bananas Musa AAA-Nanicão and Musa AAB-Terra

    Directory of Open Access Journals (Sweden)

    M.C.J. Freitas

    2005-06-01

    Full Text Available O amido de bananas tem sido pesquisado na área de nutrição a partir da introdução do conceito de Amido Resistente. O amido de Musa AAA-Nanicão e Musa AAB-Terra foram caracterizados quanto as suas respostas fisiológicas [12]. Em continuidade, o presente trabalho estudou características físicas e morfológicas dos grânulos de amido de ambas as espécies de banana comparando-as com amido nativo de milho comercial. O amido de bananas foi extraído segundo CHIANG, CHU & CHU [3]. A morfologia dos grânulos foi realizada após tratamento enzimático in vitro a 37°C/24h com alfa-amilase pancreática. Foram efetuados os respectivos amilogramas e difractogramas de raios-X. Os grânulos de amido da Musa Tipo AAA-Nanicão apresentaram comprimento entre 30-40µm. Em Musa AAB-Terra, os grânulos, também ovais e alongados, eram um pouco menores, 20-30µm. A corrosão enzimática in vitro iniciava-se sobre a superfície anteriormente lisa e formavam estrias superficiais e apicais. A Microscopia Eletrônica de Varredura (MEV mostrou que a hidrólise in vitro por 24 horas foi pequena e ocorria sobretudo nas camadas amorfas dos grânulos de ambas as espécies. O padrão de corrosão demonstrou-se distinto daquele ocorrido no amido de milho. As suspensões de amido de bananas ao viscosímetro demonstraram forte capacidade de hidratação e menor capacidade de retrogradação em relação ao milho, sobretudo do amido de Musa Tipo AAA-Nanicão; o amido de Musa Tipo AAB-Terra apresentou maior estabilidade de pasta. Na análise de difração de raios-X, os grânulos de bananas apresentaram padrão tipo B e C para Musa Tipo AAA-Nanicão e Musa Tipo AAB-Terra, respectivamente. Conclui-se que os amidos de Musa AAA-Nanicão e Musa AAB-Terra são estruturalmente distintos, justificando as respostas fisiológicas distintas encontradas posteriormente pelos mesmos autores. As distinções das propriedades físicas e bioquímicas obtidas para os grânulos, embora

  13. Pectinase production by Aspergillus niger using banana (Musa balbisiana) peel as substrate and its effect on clarification of banana juice.

    Science.gov (United States)

    Barman, Sumi; Sit, Nandan; Badwaik, Laxmikant S; Deka, Sankar C

    2015-06-01

    Optimization of substrate concentration, time of incubation and temperature for crude pectinase production from A. niger was carried out using Bhimkol banana (Musa balbisiana) peel as substrate. The crude pectinase produced was partially purified using ethanol and effectiveness of crude and partially purified pectinase was studied for banana juice clarification. The optimum substrate concentration, incubation time and temperature of incubation were 8.07 %, 65.82 h and 32.37 °C respectively, and the polygalacturonase (PG) activity achieved was 6.6 U/ml for crude pectinase. The partially purified enzyme showed more than 3 times of polygalacturonase activity as compared to the crude enzyme. The SDS-PAGE profile showed that the molecular weight of proteins present in the different pectinases varied from 34 to 42 kDa. The study further revealed that highest clarification was achieved when raw banana juice was incubated for 60 min with 2 % concentration of partially purified pectinase and the absorbance obtained was 0.10.

  14. Avaliação do estado nutricional e do desenvolvimento da bananeira-prata-anã (Musa spp. em função da adubação nitrogenada Evaluation of the nutricional status and development of banana cultivar "prata anã" (Musa spp. in relation to nitrogen fertilization

    Directory of Open Access Journals (Sweden)

    Patrícia Soares Furno Fontes

    2003-04-01

    Full Text Available O experimento foi instalado num Argissolo Amarelo, no município de Itaocara-RJ (Latitude de 21º 39' 12" Sul e Longitude de 42º 04' 36" Oeste, com clima conforme Köppen, classificado como Awi, temperatura média anual em torno de 22,5ºC e precipitação anual de 1041 mm, com o objetivo de avaliar o estado nutricional e o desenvolvimento da bananeira cultivar Prata-Anã em função da aplicação de cinco doses de nitrogênio (0; 150; 300; 450 e 600 kg ha-1 ano-1 de N. Os teores dos nutrientes na matéria seca foliar não foram alterados pelas doses de N, exceções feitas ao Mn e ao Cl. Os teores foliares dos nutrientes variaram entre as épocas analisadas, com exceção do Ca. Não houve diferença nos teores de N na folha analisada, em função do incremento da adubação nitrogenada. Foram encontrados menores teores de K na matéria seca foliar, na época da inflorescência e formação do cacho. A adubação nitrogenada não afetou a época de florescimento e a altura do cacho. Observou-se diminuição na emissão de filhotes com o incremento das doses de N.The experiment was carried in Yellow Podzolic at Itaocara, Rio de Janeiro State, Brazil, (Latitude 21º 39' 12" South and Longitude 42º 04' 36" West, with climate, conform Köppen, classified as Awi,, to evaluate the nutritional status and the development of the banana cultivar "Prata Anã" in relation to five nitrogen fertilization levels (0, 150, 300, 450 and 600 kg ha-1 year-1 of N. In general, the analyzed nutrients contents in the leaf dry matter were not modified by the increase of N dosage, being exception Mn and Cl. The levels of leaf nutrients differed between sampling seasons, with the exception of Ca. There was no variation in the levels of N in the leaf dry matter, in relation to nitrogen fertilization. Lower levels of K were observed in the leaf dry matter during the blooming and bunch development. In the first growing cycle, the nitrogen fertilization had no effect

  15. Comparative Phosphoproteomics Reveals an Important Role of MKK2 in Banana (Musa spp.) Cold Signal Network

    Science.gov (United States)

    Gao, Jie; Zhang, Sheng; He, Wei-Di; Shao, Xiu-Hong; Li, Chun-Yu; Wei, Yue-Rong; Deng, Gui-Ming; Kuang, Rui-Bin; Hu, Chun-Hua; Yi, Gan-Jun; Yang, Qiao-Song

    2017-01-01

    Low temperature is one of the key environmental stresses, which greatly affects global banana production. However, little is known about the global phosphoproteomes in Musa spp. and their regulatory roles in response to cold stress. In this study, we conducted a comparative phosphoproteomic profiling of cold-sensitive Cavendish Banana and relatively cold tolerant Dajiao under cold stress. Phosphopeptide abundances of five phosphoproteins involved in MKK2 interaction network, including MKK2, HY5, CaSR, STN7 and kinesin-like protein, show a remarkable difference between Cavendish Banana and Dajiao in response to cold stress. Western blotting of MKK2 protein and its T31 phosphorylated peptide verified the phosphoproteomic results of increased T31 phosphopeptide abundance with decreased MKK2 abundance in Daojiao for a time course of cold stress. Meanwhile increased expression of MKK2 with no detectable T31 phosphorylation was found in Cavendish Banana. These results suggest that the MKK2 pathway in Dajiao, along with other cold-specific phosphoproteins, appears to be associated with the molecular mechanisms of high tolerance to cold stress in Dajiao. The results also provide new evidence that the signaling pathway of cellular MKK2 phosphorylation plays an important role in abiotic stress tolerance that likely serves as a universal plant cold tolerance mechanism. PMID:28106078

  16. Mineral fertilizers improve the sensory quality of East African Highland bananas (Musa AAA-EA, cv. Kisansa)

    NARCIS (Netherlands)

    Taulya, G.; Asten, van P.J.A.; Nowakunda, K.; Kaddu-Mukasa, P.

    2010-01-01

    Some farmers in Uganda believe that fertilizers negatively affect the sensory attributes of cooking type bananas. This belief may hamper the adoption of fertilizers. To verify the validity of this belief, bunches (Musa AAA-EA, cv. ‘Kisansa’) from fertilized (i.e. N-P-K-Mg-Zn-S-B-Mo) and non-fertiliz

  17. Mineral fertilizer response and nutrient use efficiencies of East African highland banana (Musa spp., AAA-EAHB, cv. Kisansa)

    NARCIS (Netherlands)

    Nyombi, K.; Asten, van P.J.A.; Corbeels, M.; Taulya, G.; Leffelaar, P.A.; Giller, K.E.

    2010-01-01

    Poor yields of East African highland bananas (Musa spp., AAA-EAHB) on smallholder farms have often been attributed to problems of poor soil fertility. We measured the effects of mineral fertilizers on crop performance at two sites over two to three crop cycles; Kawanda in central Uganda and Ntungamo

  18. Characterization of some physicist, mechanics and chemistries properties in the banana (Musa spp.

    Directory of Open Access Journals (Sweden)

    Carlos Modesto Martínez Hernández

    2016-10-01

    Full Text Available The present work approaches the study of some physical-mechanical and chemical properties of the banana (Musa spp.. For its investigation, they took samples in the Municipal Company of Cultivos Varios, municipality Taguasco, provinces of Sancti-Spíritus. They were carried out rehearsals related to some physical, mechanical and chemical variables as: pH, oBrix, total regular acids, static coefficients of friction and mechanical impact damages deal with the established norms. The objective of the work it related to the evaluation of the physical – mechanical and chemical parameters of the banana (Musa spp. for a good postharvest manage. Inside the main obtained results they stand out: longitude half 20.85 cm; circumference 11.25 cm; the depth of the shell 0.25 cm; the depth of the pulp 2.85 cm. The total soluble solids (SST they oscillated between 13 and 16 oBrix; the total regular acidity oscillates between 0, 2 and 1, 6 meq/100 g malic acid. The pH oscillates between 4.42 and 5.27. The content of humidity of the pulp oscillates between 82 and 86 %; while in the shell this value oscillates between 14 and 18%. The impact rehearsals showed little resistance to impacts to the order of 105,94 (J, that which makes that the fruit is very susceptible to mechanical damages, together with their qualities of little shelf life (6 days postharvest. The results in the analyzed banana have been always using the first two hands of the cluster with a range from 1 to 9 days later in the crop of the fruits.

  19. Study of Post-Harvest Ambon Banana (Musa acuminata) Preservation Using X-Ray

    Science.gov (United States)

    Dwijananti, P.; Handayani, L.; Marwoto, P.; Iswari, R. S.

    2016-08-01

    An exposure to Ambon banana (Musa Acuminata) samples has been done by using X-rays with current, voltage and exposure time are control parameters. This study aimed to determine storage ability of the post-harvest sample. Five samples were exposured by x-rays with the dose of (3-5) × 10-14 Gy. The samples were stored at room temperature. Their mass and physical condition (color and smell) were evaluated every 3 days. It was found that the control sample which was not exposured by X-ray was ripe in the sixth day indicated by the yellow color and good smell of the banana. Meanwhile, the samples which were exposured by (3 - 5) × 10-14 Gy doze of X-ray looked fresher and still had green color. These samples showed their ripening in the ninth day and their mass decrease was (12-13)% which is lower than the control sample. The preservation of banana can be done through low-dose X-ray exposure.

  20. EIN3-like gene expression during fruit ripening of Cavendish banana (Musa acuminata cv. Grande naine).

    Science.gov (United States)

    Mbéguié-A-Mbéguié, Didier; Hubert, Olivier; Fils-Lycaon, Bernard; Chillet, Marc; Baurens, Franc-Christophe

    2008-06-01

    Ethylene signal transduction initiates with ethylene binding at receptor proteins and terminates in a transcription cascade involving the EIN3/EIL transcription factors. Here, we have isolated four cDNAs homologs of the Arabidopsis EIN3/EIN3-like gene, MA-EILs (Musa acuminata ethylene insensitive 3-like) from banana fruit. Sequence comparison with other banana EIL gene already registered in the database led us to conclude that, at this day, at least five different genes namely MA-EIL1, MA-EIL2/AB266318, MA-EIL3/AB266319, MA-EIL4/AB266320 and AB266321 exist in banana. Phylogenetic analyses included all banana EIL genes within a same cluster consisting of rice OsEILs, a monocotyledonous plant as banana. However, MA-EIL1, MA-EIL2/AB266318, MA-EIL4/AB266320 and AB266321 on one side, and MA-EIL3/AB266319 on the other side, belong to two distant subclusters. MA-EIL mRNAs were detected in all examined banana tissues but at lower level in peel than in pulp. According to tissues, MA-EIL genes were differentially regulated by ripening and ethylene in mature green fruit and wounding in old and young leaves. MA-EIL2/AB266318 was the unique ripening- and ethylene-induced gene; MA-EIL1, MA-EIL4/Ab266320 and AB266321 genes were downregulated, while MA-EIL3/AB266319 presented an unusual pattern of expression. Interestingly, a marked change was observed mainly in MA-EIL1 and MA-EIL3/Ab266319 mRNA accumulation concomitantly with changes in ethylene responsiveness of fruit. Upon wounding, the main effect was observed in MA-EIL4/AB266320 and AB266321 mRNA levels, which presented a markedly increase in both young and old leaves, respectively. Data presented in this study suggest the importance of a transcriptionally step control in the regulation of EIL genes during banana fruit ripening.

  1. Variable Number of Tandem Repeat Markers in the Genome Sequence of Mycosphaerella Fijiensis, the Causal Agent of Black Leaf Streak Disease of Banana (Musa spp.)

    Science.gov (United States)

    Mycosphaerella fijiensis, the causal agent of banana leaf streak disease (commonly known as black Sigatoka), is the most devastating pathogen attacking bananas (Musa spp). Recently the whole genome sequence of M. fijiensis became available. This sequence was screened for the presence of Variable Num...

  2. Avaliação de genótipos de bananeira à Colletotrichum musae em pós-colheita Evaluation of genotypes of banana to Colletotrichum musae in postharvest

    Directory of Open Access Journals (Sweden)

    Danilo Batista Pinho

    2010-09-01

    Full Text Available Alguns hibridos tetraploides apresentam resistência à antracnose, porém são reduzidas as informações sobre os níveis de resistência desses genótipos e o comportamento dos mesmos no Brasil. O objetivo do presente trabalho foi avaliar a incidência e a severidade de antracnose sobre o comportamento de diferentes genótipos de bananeiras resistentes a Sigatoka-Negra em função de diferentes concentrações de conídios de Colletotrichum musae em diferentes épocas de avaliação. Os frutos colhidos no estádio de maturação fisiológica dos genótipos, Thap Maeo, Caipira, Pacovan Ken, Ambrosia, PV 42-53, PA 42-44, FHIA 01, FHIA 18 e a cultivar Prata-Anã utilizada como testemunha. Buquês contendo três frutos foram atomizados com diferentes concentrações (0; 10²; 10³; 10(4; 10(5 e 10(6 conídios/mL de C. musae. Os frutos foram incubados a 25 ºC. A incidência e severidade da antracnose foi avaliada aos 3; 6; 9 e 12 dias após a inoculação. Foi utilizado o delineamento inteiramente casualizado, em esquema fatorial 9x6x4 (genótipos, concentrações de esporos e épocas de avaliação, com quatro repetições, contendo quatro buquês de três frutos. Aos doze dias de avaliação, todos os frutos de todos os genótipos estudados apresentaram incidência de C. musae quando inoculados com as diferentes concentrações de inóculo. A cultivar Thap Maeo apresentou a menor severidade (27,5% de antracnose, enquanto os genótipos Ambrosia, PV 42-53, FHIA 18 e FHIA 01 foram as mais suscetíveis à antracnose com 64%, 64%, 61,33% e 58% de severidade, respectivamente.Some tetraploides hybrids present resistance to anthracnose, however the information on the levels of resistance of these genotypes and the behavior in Brazil are reduced. The aim of this study was to evaluate the incidence and severity of injury on the behavior of different banana genotypes resistant to Black Sigatoka for different concentrations of conidia of Colletotrichum

  3. Molecular Characterization of Banana streak virus Isolate from Musa Acuminata in China

    Institute of Scientific and Technical Information of China (English)

    Jun Zhuang; Jian-hua Wang; Xin Zhang; Zhi-xinLiu

    2011-01-01

    Banana streak virus (BSV),a member of genus Badnavirus,is a causal agent of banana streak disease throughout the world.The genetic diversity of BSVs from different regions of banana plantations has previously been investigated,but there are relatively few reports of the genetic characteristic of episomal (non-integrated)BSV genomes isolated from China.Here,the complete genome,a total of 7722bp (GenBank accession number DQ092436),of an isolate of Banana streak virus (BSV) on cultivar Cavendish (BSAcYNV) in Yunnan,China was determined.The genome organises in the typical manner of badnaviruses.The intergenic region of genomic DNA contains a large stem-loop,which may contribute to the ribosome shift into the following open reading frames (ORFs).The coding region of BSAcYNV consists of three overlapping ORFs,ORF 1 with a non-AUG start eodon and ORF2 encoding two small proteins are individually involved in viral movement and ORF3 encodes a polyprotein.Besides the complete genome,a defective genome lacking the whole RNA leader region and a majority of ORF1 and which encompasses 6525bp was also isolated and sequenced from this BSV DNA reservoir in infected banana plants.Sequence analyses showed that BSAcYNV has closest similarity in terms of genome organization and the coding assignments with an BSV isolate from Vientam (BSAcVNV).The corresponding coding regions shared identities of 88% and ~95% at nucleotide and amino acid levels,respectively.Phylogenetic analysis also indicated BSAcYNV shared the closest geographical evolutionary relationship to BSAcVNV among sequenced banana streak badnaviruses.

  4. Temperature effects on peel spotting in "Sucrier banana" fruit

    NARCIS (Netherlands)

    Trakulnaleumsai, C.; Ketsa, S.; Doorn, van W.G.

    2006-01-01

    Banana fruit of the cultivar `Sucrier¿ (Musa acuminata, AA Group) develops peel spotting at a relatively early stage of development (when the peel is about as slightly more yellow than green). Holding ripening bananas at 15 and 18 °C instead of room temperature (26¿27 °C) only temporarily reduced sp

  5. Variabilidade genética de genótipos de bananeira (Musa spp submetidos ao estresse salino Genetic variability of banana (Musa spp genotypes subjected to saline stress

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    Eline W. F. Gomes

    2005-06-01

    Full Text Available O Brasil é o segundo maior produtor mundial de bananas, sendo Pernambuco o estado que apresenta maior expansão da cultura na região do perímetro irrigado do Vale do São Francisco em cujas áreas, porém, são freqüentes os problemas de salinização do solo o que se pode tornar um fator limitante para a cultura. A utilização de cultivares tolerantes à salinidade apresenta-se como uma alternativa bastante viável; assim, identificar genótipos que se adaptem a solos salinos da Região Nordeste, é de fundamental importância para os programas de melhoramento. Este trabalho teve por finalidade utilizar marcadores moleculares, obtidos por amplificação de DNA via Reação em Cadeia polimerase (PCR com iniciadores (primers de RAPD, para determinar a variabilidade genética entre dez genótipos de banana (Musa spp: Pacovan, Nanicão, Caipira, FHIA18, Calcuttá, SN/2, Borneo, M-53, Microcarpa e Lidi, correlacionando-os com a tolerância ao estresse salino. Foram testados 25 primers. O iniciador D0142A07 gerou o maior número de loci polimórficos, enquanto o D0142B05 originou o menor. Em geral, o polimorfismo gerado com os marcadores de DNA mostrou que, apesar da base genética estreita, no caso das que são formadas pelo mesmo grupo genômico, os genótipos de bananeira apresentam variabilidade genética relativamente alta. As variedades que apresentaram maior tolerância ao estresse salino, como a Pacovan e SN/2, mostraram-se distantes geneticamente, quando comparadas com as mais sensíveis ao sal, como Calcuttá e Lidi.Brazil is the second lagest banana producer. The State of Pernambuco has presented the greatest expansion of banana cultivation in the irrigated perimeters of the São Francisco Valley. In these areas, however, there are frequent problems with high salt content in the soil, which could turn out to be a major limiting factor to its cultivation. The utilization of cultivars tolerant to saline conditions is a rather viable

  6. Pesticide residues in heterogeneous plant populations, a model-based approach applied to nematicides in banana (Musa spp.).

    Science.gov (United States)

    Tixier, Philippe; Chabrier, Christian; Malézieux, Eric

    2007-03-21

    Nematicides are widely used to control plant-parasitic nematodes in intensive export banana (Musa spp.) cropping systems. Data show that the concentration of fosthiazate in banana fruits varies from zero to 0.035 g kg-1, under the maximal residue limit (MRL=0.05 mg kg-1). The fosthiazate concentration in fruit is described by a Gaussian envelope curve function of the interval between pesticide application and fruit harvest (preharvest interval). The heterogeneity of phenological stages in a banana population increases over time, and thus the preharvest interval of fruits harvested after a pesticide application varies over time. A phenological model was used to simulate the long-term harvest dynamics of banana at field scale. Simulations show that the mean fosthiazate concentration in fruits varies according to nematicide application program, climate (temperature), and planting date of the banana field. This method is used to assess the percentage of harvested bunches that exceed a residue threshold and to help farmers minimize fosthiazate residues in bananas.

  7. Senescent spotting of banana peel is inhibited by modified atmosphere packaging

    NARCIS (Netherlands)

    Choehom, R.; Ketsa, S.; Doorn, van W.G.

    2004-01-01

    Banana fruit (Musa cavendishii [Musa acuminata] AA Group cv. Sucrier) were placed in trays and held at 29-30 degreesC. Covering the trays with 'Sun wrap' polyvinyl chloride film prevented the early senescent peel spotting, typical for this cultivar. Carbon dioxide and ethylene concentrations within

  8. Roseomonas musae sp. nov., a new bacterium isolated from a banana phyllosphere.

    Science.gov (United States)

    Nutaratat, Pumin; Srisuk, Nantana; Duangmal, Kannika; Yurimoto, Hiroya; Sakai, Yasuyoshi; Muramatsu, Yuki; Nakagawa, Yasuyoshi

    2013-03-01

    A Gram-negative, coccobacilli, non-spore forming and non-motile bacterium, designated PN1(T), was isolated from a banana leaf collected in Mattra island, Thailand. This isolate was observed to grow optimally at 30 °C and pH 7.0, and to grow with 0-3 % NaCl. Comparative 16S rRNA gene sequence analysis showed that strain PN1(T) is closely related to members of the genus Roseomonas, exhibiting the highest 16S rRNA gene sequence similarity to Roseomonas aestuarii JC17(T) (96.5 %). The DNA G + C content of strain PN1(T) was determined to be 69.7 mol %. Based on physiological and biochemical tests, and genotypic differences between strain PN1(T) and the validly named species of the genus Roseomonas, it is proposed that the strain be classified as a new species of Roseomonas for which the name Roseomonas musae sp. nov. is proposed. The type strain is PN1(T) (= BCC 44863(T) = NBRC 107870(T)).

  9. Mechanisms of haplotype divergence at the RGA08 nucleotide-binding leucine-rich repeat gene locus in wild banana (Musa balbisiana

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    Miller Robert NG

    2010-07-01

    Full Text Available Abstract Background Comparative sequence analysis of complex loci such as resistance gene analog clusters allows estimating the degree of sequence conservation and mechanisms of divergence at the intraspecies level. In banana (Musa sp., two diploid wild species Musa acuminata (A genome and Musa balbisiana (B genome contribute to the polyploid genome of many cultivars. The M. balbisiana species is associated with vigour and tolerance to pests and disease and little is known on the genome structure and haplotype diversity within this species. Here, we compare two genomic sequences of 253 and 223 kb corresponding to two haplotypes of the RGA08 resistance gene analog locus in M. balbisiana "Pisang Klutuk Wulung" (PKW. Results Sequence comparison revealed two regions of contrasting features. The first is a highly colinear gene-rich region where the two haplotypes diverge only by single nucleotide polymorphisms and two repetitive element insertions. The second corresponds to a large cluster of RGA08 genes, with 13 and 18 predicted RGA genes and pseudogenes spread over 131 and 152 kb respectively on each haplotype. The RGA08 cluster is enriched in repetitive element insertions, in duplicated non-coding intergenic sequences including low complexity regions and shows structural variations between haplotypes. Although some allelic relationships are retained, a large diversity of RGA08 genes occurs in this single M. balbisiana genotype, with several RGA08 paralogs specific to each haplotype. The RGA08 gene family has evolved by mechanisms of unequal recombination, intragenic sequence exchange and diversifying selection. An unequal recombination event taking place between duplicated non-coding intergenic sequences resulted in a different RGA08 gene content between haplotypes pointing out the role of such duplicated regions in the evolution of RGA clusters. Based on the synonymous substitution rate in coding sequences, we estimated a 1 million year

  10. Incidência de fungos em pós-colheita de banana 'Prata anã'(Musa AAB Incidence of mushroons in post harvest of banana (Musa spp. 'Prata Anã' (AAB

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    Wilson da Silva Moraes

    2006-03-01

    Full Text Available Este trabalho teve por objetivo determinar a ocorrência e a freqüência de fungos em banana 'Prata anã' e elucidar o agente causal das podridões em pós-colheita de frutos provenientes do norte de Minas Gerais. Dois métodos de isolamento foram adotados: diluição em placas, a partir da lavagem de frutos verdes, e direto de frutos maduros. Os fungos Colletotrichum musae, Trichoderma harzianum, Fusarium equisetii, Penicillium sp. Aspergillus parasiticus, Trichothecium roseum, Colletotrichum acutatum, Alternaria sp., Cladosporium musae e Curvularia lunata foram os mais freqüentemente associados aos frutos. A patogenicidade desses fungos foi testada pela substituição de discos da casca de frutos verdes por discos de micélio. Colletotrichum musae apresentou área média lesionada em torno do ponto de inoculação igual a 5,8 cm², enquanto para os demais fungos testados não passou de 1,50 cm². Os resultados mostraram que C. musae é o agente primário das podridões dos frutos examinados com 100 % de incidência e os demais fungos limitaram-se a necrosar os ferimentos em torno do ponto de inoculação. O modo de infecção latente, causada por C. musae, parece favorecer, primeiramente, a colonização interna dos tecidos e, posteriormente, a ação dos fungos oportunistas, que aceleram as podridões nos frutos e na coroa.The objetive of this work were to determine the occurrence and frequency of fungus in 'Prata anã' banana and to elucidate the causal agent of post harvest rots on fruits from Jaíba, Minas Gerais, Brazil. Two isolation methods were used: diluition in placs from whashed green fruits and direct of mature fruits. The fungus Colletotrichum musae, Trichoderma harzianum, Fusarium equisetii, Penicillium sp., Aspergillus parasiticus, Trichothecium roseum, Colletotrichum acutatum, Alternaria sp., Cladosporium musae and Curvularia lunata were the more freqüentilly associated to fruits. The fungus pathogenicity were tested by

  11. Separation and identification of Musa acuminate Colla (banana) leaf proteins by two-dimensional gel electrophoresis and mass spectrometry.

    Science.gov (United States)

    Lu, Y; Qi, Y X; Zhang, H; Zhang, H Q; Pu, J J; Xie, Y X

    2013-12-19

    To establish a proteomic reference map of Musa acuminate Colla (banana) leaf, we separated and identified leaf proteins using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS). Tryptic digests of 44 spots were subjected to peptide mass fingerprinting (PMF) by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS. Three spots that were not identified by MALDI-TOF MS analysis were identified by searching against the NCBInr, SwissProt, and expressed sequence tag (EST) databases. We identified 41 unique proteins. The majority of the identified leaf proteins were found to be involved in energy metabolism. The results indicate that 2D-PAGE is a sensitive and powerful technique for the separation and identification of Musa leaf proteins. A summary of the identified proteins and their putative functions is discussed.

  12. Efeito da temperatura e período de molhamento sobre o desenvolvimento de lesões de Colletotrichum musae em banana Effect of temperature and wet period on the development of Colletotrichum musae lesions in banana

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    Wagner Rogério Leocádio Soares Pessoa

    2007-06-01

    Full Text Available A banana é a segunda fruta mais consumida no mundo, porém do campo até o mercado consumidor algo em torno de 40 % é perdido devido entre outras causas as doenças pós-colheita e a mais significativa é a antracnose. Diante da necessidade do conhecimento de fatores ambientais que condicionam estas perdas, o trabalho objetivou avaliar métodos de inoculação (com discos de BDA e estruturas do patógeno e suspensão de conídios com e sem ferimento e a influência da temperatura (10,15,20,25 e 30 ºC e do período de molhamento (0, 12, 24 e 36 h sobre o desenvolvimento de Colletotrichum musae em banana. As frutas foram inoculadas com 17 isolados de C. musae onde todos mostraram-se patogênicos quando inoculados com ferimento independentemente do tipo de inóculo utilizado. No experimento envolvendo temperatura e período de molhamento, utilizou-se três isolados de C. musae, MAG2, SFV1 e FSA, que se comportaram como mais agressivo, intermediário e pouco agressivo, respectivamente. As temperaturas em torno de 20, 25 e 30 ºC e os períodos de molhamento testados favoreceram um maior desenvolvimento de lesões, sendo as maiores lesões observadas em temperaturas ao redor de 25 e 30 ºC, com redução à medida que ocorria uma diminuição da temperatura para todos os isolados testados. A temperatura em torno de 15 ºC proporcionou o menor desenvolvimento da doença.,The banana is the second more consumed fruit in the world, but there is a product loss of about 40% from field to consuming market due to several factors including the postharvest diseases, being the anthracnose most significative among them. Considering the necessity of studying the environmental factors that affect this losses, the objective of this work was to evaluate methods of inoculation (PDA discs with pathogen structures and suspension of conidia with and without wound and the influence of temperature (10, 15, 20, 25 and 30ºC and wet period (0, 12, 24 and 36 hours on the

  13. Dried, ground banana plant leaves (Musa spp.) for the control of Haemonchus contortus and Trichostrongylus colubriformis infections in sheep.

    Science.gov (United States)

    Gregory, L; Yoshihara, E; Ribeiro, B L M; Silva, L K F; Marques, E C; Meira, E B S; Rossi, R S; Sampaio, P H; Louvandini, H; Hasegawa, M Y

    2015-12-01

    To evaluate the anthelmintic effect of Musa spp. leaves, 12 animals were artificially infected with Haemonchus contortus, and another 12 animals were infected with Trichostrongylus colubriformis. Then, both treatment groups were offered 400 g of dried ground banana plant leaves, and the control animals were offered only 1000 g of coast cross hay. During the trials, the animals received weekly physical examinations. The methods used to evaluate the efficiency of this treatment were packed cell volume, total plasma protein and faecal egg counts, and egg hatchability tests were performed on days -2, +3, +6, +9, +13 and +15. Coproculture tests were performed on day -2 to confirm monospecific infections. In the FEC and EHT, a statistically significant difference (0.04, 0.005; p  0.05) for Haemochus contortus group in all tests. Our results confirmed previous findings suggesting that dried ground banana plant leaves possess anthelmintic activity.

  14. Ripening of fruits of 'Dwarf Prata' banana (Musa acuminata x Musa balbisiana, AAB group)irradiated and treated with calcium carbide

    Energy Technology Data Exchange (ETDEWEB)

    Martineli, Maristella [Instituto de Quimica. Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ (Brazil); Coneglian, Regina C.C.; Vasconcellos, Marco A.S.; Silva, Eduardo, E-mail: rccconeg@ufrrj.br, E-mail: masv@ufrrj.br [Departamento de Fitotecnia. Instituto de Agronomia. Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropedica, RJ (Brazil); Vital, Helio C., E-mail: vital@ctex.eb.br [Secao de Defesa Nuclear. Divisao de Defesa Quimica, Biologica e Nuclear. Centro Tecnologico do Exercito (CTEx), Guaratiba, Rio de Janeiro, RJ (Brazil)

    2011-07-01

    The competing effects resulting from the exposure of fruits of 'warf prata' banana (Musa acuminata x Musa balbisiana, AAB group) to gamma radiation and to calcium carbide have been investigated in this work. The fruits were harvested in a pre-climateric stage (green colored though physiologically developed) in the city of Jaiba, state of Minas Gerais, and gamma irradiated with doses of 0.25 or 0.50 kGy in a research irradiating facility at the Brazilian Army Technology Center (CTEx) in the city of Rio de Janeiro. Some samples were also exposed to calcium carbide for 32 hours in order to accelerate ripening. Quantitative estimates of peel color, disease index and fresh mass loss were performed for 9 days while the fruits were kept at an average temperature of 23 deg C. The analyses were performed in the Federal Rural University of Rio de Janeiro, located in the city of Seropedica. The following treatments or combination of processes have been tested: untreated (control); treated only with irradiation with doses of 0.25 kGy or 0.50 kGy; treated with irradiation with doses of 0.25 kGy or 0.50 kGy and then exposed to calcium carbide. The fruits treated solely with irradiation with 0.25 kGy exhibited a better response during the first days of storage, although their initial green coloration vanished with time. In addition, the fungi Colletotrichum musae and Lasidioplodia theobroma were detected in samples submitted to the combination of both processes. In contrast, such fungi were not observed in fruits that had only been exposed to 0.25 kGy and exhibited low disease indices. Also, 1-2 cm lesions were detected on fruits.(author)

  15. Effects of gamma irradiation followed by climatization on the quality of 'Prata' banana (Musa acuminata x Musa balbisiana, AAB)

    Energy Technology Data Exchange (ETDEWEB)

    Martineli, Maristella; Coneglian, Regina C.C.; Vasconcellos, Marco A.S.; Silva, Eduardo; Rocha, Janielio G.; Melo, Maruzanete P. [Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropedica, RJ. Inst. de Agronomia (Brazil)], e-mail: maristellamartineli@yahoo.com.br; Vital, Helio C. [Centro Tecnologico do Exercito (CTEx), Rio de Janeiro, RJ (Brazil). Secao de Defesa Nuclear], e-mail: vital@ctex.eb.br

    2009-07-01

    Banana is a highly perishable climacteric fruit. In order to ensure fast and homogeneous ripening that will make its processing and commercialization easier, it is usually treated by climatization. On the other hand, irradiation is an interesting physical process capable of delaying ripening and extending the shelf life of fruits. This work investigated the competing effects of irradiation followed by climatization on the quality of 'Prata' banana (Musa acuminata x Musa balbisiana, AAB). All fruits were harvested in the preclimacteric stage, rinsed and stored at 27 deg C after being submitted to five different treatments. After hygienization, four out of five samples were exposed to gamma-ray at doses of 0.25 and 0.50 kGy. The unirradiated fruits were left for control and half of the irradiated samples were then climatized by exposure to CaC{sub 2}. Measurements of fresh mass loss, total soluble solids, peel coloration and disease index were performed on five different dates for up to nine days in order to monitor quality and the degree of ripening. It was found that high temperatures prevailing during climatization and storage accelerated maturation in all fruits in spite of the use of irradiation, with the control becoming ripe in 3 days only. In addition, based on the consistent results from the experiments performed, it can be concluded that irradiation at the doses tested was unable to overcome the maturation effects produced by climatization. However, the sole use of irradiation with 0.25 kGy yielded the lowest figures for fresh mass loss and disease index in the samples, thus becoming the most attractive among the treatments tested. (author)

  16. [Establishment of embryogenic cell suspension culture and plant regeneration of edible banana Musa acuminata cv. Mas (AA)].

    Science.gov (United States)

    Wei, Yue-Rong; Huang, Xue-Lin; Li, Jia; Huang, Xia; Li, Zhe; Li, Xiao-Ju

    2005-01-01

    Conventional breeding for dual resistance of disease and pest of Musa cultivars remains a difficult endeavor, as the plant is polyploidic and high in sterility. Biotechnological techniques, eg., genetic engineering, in vitro mutation breeding, or protoplast fusion, may overcome the difficulties and improve the germplasm. Establishment of a stable embryogenic cell suspension (ECS) is a prerequisite for any of the biotechnological breeding methods. In this study an embryogenic cell suspension was established from immature male flower of Musa acuminata cv. Mas (AA), a popular commercial variety of banana in the South-East Asian region. After culture for 5-6 months on callus induction media, which consisted of MS salts, different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), 4.1 micromol/L biotin, 5.7 micromol/L indoleacetic acid (IAA), 5.4 micromol/L naphthaleneacetic acid (NAA), other vitamins, 87 mmol/L sucrose, and solidified with 7 g/L agarose, meristematic globules and yellow, friable embryogenic cultures were induced from the explants of 1-15th row young floral hands of immature male flowers. Of the four treatments of 2,4-D, 9 micromol/L was the most effective on the callus induction, it transformed 40.96% and 7.45% of the cultivated male floral hands into callus and embryogenic callus respectively. The explants to produce highest frequency of the embryogenic calli were floral hands of 6 to 12th rows, which generated 5.79% of the embryogenic calli. Suspension cultures were initiated from these embryogenic calli in liquid medium supplemented with 4.5 micromol/L 2, 4-D. After sieving selection of the cultures using a stainless steel metallic strainer with pore sizes of 154 microm at 15 day intervals for 3 months, homogeneous and yellow embryogenic cell suspensions, composed of single cells and small cell aggregates, were established. Based upon the growth quantity and growth rate of ECS, it was determined that the appropriate inoculum was 2.0 mL PCV

  17. Phenalenone-type phytoalexins mediate resistance of banana plants (Musa spp.) to the burrowing nematode Radopholus similis.

    Science.gov (United States)

    Hölscher, Dirk; Dhakshinamoorthy, Suganthagunthalam; Alexandrov, Theodore; Becker, Michael; Bretschneider, Tom; Buerkert, Andreas; Crecelius, Anna C; De Waele, Dirk; Elsen, Annemie; Heckel, David G; Heklau, Heike; Hertweck, Christian; Kai, Marco; Knop, Katrin; Krafft, Christoph; Maddula, Ravi K; Matthäus, Christian; Popp, Jürgen; Schneider, Bernd; Schubert, Ulrich S; Sikora, Richard A; Svatoš, Aleš; Swennen, Rony L

    2014-01-01

    The global yield of bananas-one of the most important food crops-is severely hampered by parasites, such as nematodes, which cause yield losses up to 75%. Plant-nematode interactions of two banana cultivars differing in susceptibility to Radopholus similis were investigated by combining the conventional and spatially resolved analytical techniques (1)H NMR spectroscopy, matrix-free UV-laser desorption/ionization mass spectrometric imaging, and Raman microspectroscopy. This innovative combination of analytical techniques was applied to isolate, identify, and locate the banana-specific type of phytoalexins, phenylphenalenones, in the R. similis-caused lesions of the plants. The striking antinematode activity of the phenylphenalenone anigorufone, its ingestion by the nematode, and its subsequent localization in lipid droplets within the nematode is reported. The importance of varying local concentrations of these specialized metabolites in infected plant tissues, their involvement in the plant's defense system, and derived strategies for improving banana resistance are highlighted.

  18. 16S ribosomal DNA characterization of nitrogen-fixing bacteria isolated from banana (Musa spp.) and pineapple (Ananas comosus (L.) Merril).

    Science.gov (United States)

    Magalhães Cruz, L; de Souza, E M; Weber, O B; Baldani, J I; Döbereiner, J; Pedrosa, F de O

    2001-05-01

    Nitrogen-fixing bacteria isolated from banana (Musa spp.) and pineapple (Ananas comosus (L.) Merril) were characterized by amplified 16S ribosomal DNA restriction analysis and 16S rRNA sequence analysis. Herbaspirillum seropedicae, Herbaspirillum rubrisubalbicans, Burkholderia brasilensis, and Burkholderia tropicalis were identified. Eight other types were placed in close proximity to these genera and other alpha and beta Proteobacteria.

  19. Microbiological and physicochemical factors affecting Aspergillus section Flavi incidence in Cavendish banana (Musa cavendishii) chips production in Southern Philippines.

    Science.gov (United States)

    Sales, A C; Azanza, P V; Yoshizawa, T

    2005-01-01

    Microbiological and physicochemical factors affecting the incidence of Aspergillus section Flavi in dried Cavendish banana (Musa cavendishii) chips production in Southern Philippines were examined. The average counts of Aspergillus section Flavi (AFC) in fresh and dried Cavendish bananas from 10 production batches of the Philippine Agro-Industrial Development Cooperative in Davao del Norte, Southern Philippines were 1.2 x 10(2) and 1.6 x 10(2) cfu/g, respectively. Isolates from both samples were identified to be Aspergillus flavus based on spore type and conidial structure of isolates. An increasing trend in the AFC of Cavendish bananas was observed during dried banana chips processing. Variability in the AFC between production batches was attributed to differences in aerobic and fungal populations and physicochemical characteristics of the fruits, peel damage of the raw materials, concentration of AFC in the air and food-contact surfaces of the production area, and temperature and relative humidity (RH) conditions of the environment during production and storage. Physicochemical characteristics of Cavendish bananas from the receipt of raw materials up to the first day of drying were within the reported range of values allowing growth and toxin production by aflatoxigenic fungi. Air-borne AFC varied depending on the section of the production area examined. The close proximity of the waste disposal area from the production operation to the preparation, drying and storage areas suggests that cross-contamination, probably air-borne or insect-borne was a likely occurrence. The hands of workers were also identified as AFC sources. Results of this study highlight the need for the development of strategies to control aflatoxigenic fungi and aflatoxin contamination in Philippine dried Cavendish bananas.

  20. Carbohydrate binding properties of banana (Musa acuminata) lectin I. Novel recognition of internal alpha1,3-linked glucosyl residues.

    Science.gov (United States)

    Mo, H; Winter, H C; Van Damme, E J; Peumans, W J; Misaki, A; Goldstein, I J

    2001-05-01

    Examination of lectins of banana (Musa acuminata) and the closely related plantain (Musa spp.) by the techniques of quantitative precipitation, hapten inhibition of precipitation, and isothermal titration calorimetry showed that they are mannose/glucose binding proteins with a preference for the alpha-anomeric form of these sugars. Both generate precipitin curves with branched chain alpha-mannans (yeast mannans) and alpha-glucans (glycogens, dextrans, and starches), but not with linear alpha-glucans containing only alpha1,4- and alpha1,6-glucosidic bonds (isolichenan and pullulan). The novel observation was made that banana and plantain lectins recognize internal alpha1,3-linked glucosyl residues, which occur in the linear polysaccharides elsinan and nigeran. Concanavalin A and lectins from pea and lentil, also mannose/glucose binding lectins, did not precipitate with any of these linear alpha-glucans. This is, the authors believe, the first report of the recognition of internal alpha1,3-glucosidic bonds by a plant lectin. It is possible that these lectins are present in the pulp of their respective fruit, complexed with starch.

  1. Determination better culture medium in the establishment phase for the in vitro propagation of banana (Musa paradisiaca L

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    Ancasi-Espejo Ruth Gabriela

    2016-08-01

    Full Text Available This research was conducted at the Laboratory of Plant Biotechnology of the Department of Biological and Natural Sciences of the Amazonian University of Pando, in 2014. The aim of the study was to determine better culture medium in the establishment phase for propagation in vitro banana (Musa paradisiaca L., 20 were selected and characterized mother plants NTRCA (New Technology Research Center Amazonia. A completely random design (CRD with three different culture media was used. The culture media were M1 Murashige and Skoog (MS was supplemented with ascorbic acid 100 mg/L and L-cysteine 2 ml /L, M2 Murashige and Skoog (MS was supplemented charcoal 2 g/L, M3 Murashige and Skoog (MS supplement-ed with ascorbic acid 100 mg/L and cítrico100 mg/L acid. The variables evaluated were: The survival of the former Plantes, where contamination and oxidation was observed. The results showed that in the first phase of establishment, the best answer for the survival of the former Plantes banana (Musa paradisiaca, was with the culture medium 3, where a lower degree of oxidation (0.26 and pollution for all explants was obtained was 28%.

  2. Efeitos do Banana streak virus no desenvolvimento de cultivares de bananeira Effects of banana streak virus on the development of banana cultivars

    Directory of Open Access Journals (Sweden)

    Daniela Garcia Silveira

    2007-06-01

    Full Text Available Este trabalho avaliou, em condições de casa de vegetação, os efeitos da infecção pelo BSV no crescimento de cinco cultivares de bananeira. Mudas micropropagadas das cultivares SH 3640, FHIA 18, Caipira, Thap Maeo e Pioneira foram inoculadas com BSV pela cochonilha Planacoccus citri Risso. Como controles utilizaram-se mudas não inoculadas e inoculadas com cochonilhas não virulíferas. Avaliou-se a altura das plantas, o diâmetro do pseudocaule, o número de folhas, a área foliar e as massas da matéria seca da parte aérea e da raiz. Os primeiros sintomas do BSV foram detectados 15 dias após a inoculação em todas as plantas inoculadas com o vírus. Houve diferenças estatísticas significativas nas variáveis analisadas, concluindo-se que o vírus afetou o desenvolvimento das plantas de todas as cultivares avaliadas.On this study, the BSV effects on five banana cultivars were evaluated under greenhouse conditions. Micropropagated plants of cultivars SH 3640, FHIA 18, Caipira, Thap Maeo and Pioneira were inoculated with BSV using mealybug Planacoccus citri Risso as a vector. Controls plants were inoculated with non-viruliferous mealybugs or not inoculated. Plant height, pseudostem diameter, number of leaves, foliar area and root and shoot dry mass were evaluated. Disease symptoms were first visible 15 days after plant inoculation with virus. Statistical differences were detected for the host growth variables evaluated. It was concluded that BSV affected significantly the growth of all studied cultivars.

  3. Register of New Fruit and Nut Cultivars List 45. Banana, cacao, Spanish lime, plantain

    Science.gov (United States)

    The Register of New Fruit and Nut Varieties 45 is a compilation of descriptions of new fruit and nut cultivars from around the world. In this edition, newly released cacao, banana, plantain, and genip cultivars are described in terms of their origins, important fruit traits and yield....

  4. Register of new fruit and nut cultivars list 48. Banana, cacao, plantain

    Science.gov (United States)

    The Register of New Fruit and Nut Varieties 48 is a compilation of descriptions of new fruit and nut cultivars from around the world. In this edition, newly released banana, plantain, and cacao cultivars are described in terms of their origins, important fruit traits and yield. ...

  5. Gibberella musae (Fusarium musae) sp. nov., a recently discovered species from banana is sister to F. verticillioides

    NARCIS (Netherlands)

    Hove, van F.; Waalwijk, C.; Logrieco, A.; Munaut, F.; Moretti, A.

    2011-01-01

    Several strains of Fusarium isolated from banana were identified previously as F. verticillioides (Sacc.) Nirenberg but described as unable to produce fumonisin. Here we report biochemical and morphological evidence, as well as multilocus phylogenetic analyses based on elongation factor (EF-1a), cal

  6. ALLOCATIVE EFFICIENCY AND RESOURCE USE IN BANANA ( Musa sapientum A ND PLANTAIN ( Musa paradisiaca PRODUCTION ENTERPRISES IN BAYELSA STATE , NIGERIA

    Directory of Open Access Journals (Sweden)

    Kainga Prince Ebiowei

    2013-11-01

    Full Text Available Present study was conducted to determine the allocative efficiency and resource use of banana and plantain production enterprises in Bayelsa State of Nigeria. Three - stage sampling technique was used for drawing the samples and getting data. Total sample size was 180 farm households. The data were analyzed by using mean and production function models. Allocative efficiency and resource use results indicates farmers incapabil ity in efficient use and allocation of banana and plantain suckers, family labor and hired labor. In relation to total revenue, the elasticity of the plantain suckers showed that, an increase in it by one unit will lead to an increase in total revenue. Results from the allocative efficiency index, objective of profit maximization and equimarginal principle, indicated that banana suckers were efficiently allocated, while plantain suckers were inefficiently allocated; family and hired labor were inefficiently allocated in both banana and plantain enterprises. While plantain suckers were under - utilized, family and hired labor were both over - utilized in both enterprises, suggesting that farmers were yet to achieve absolute degree of allocative efficiency in thes e resources. However, they could profitably reduce the quantity of family and hired labor and increase plantain suckers that they employ. Thus, allocative efficiency and resource use can be enhanced by the provision of credit/ loan, good rural roads, impro ved varieties of suckers and farming technologies, extension services and subsidized farm inputs and equipment among others by Government, Non - Governmental Organizations (NGOs and institutions alike through genuine political will

  7. Characterization of banana starches obtained from cultivars grown in Brazil.

    Science.gov (United States)

    de Barros Mesquita, Camila; Leonel, Magali; Franco, Célia Maria Landi; Leonel, Sarita; Garcia, Emerson Loli; Dos Santos, Thaís Paes Rodrigues

    2016-08-01

    The starch market is constantly evolving and studies that provide information about the physical and rheological properties of native starches to meet the diverse demands of the sector are increasingly necessary. In this study starches obtained from five cultivars of banana were analyzed for size and shape of granules, crystallinity, chemical composition, resistant starch, swelling power, solubility, thermal and paste properties. The granules of starch were large (36.58-47.24μm), oval, showed crystallinity pattern type B and the index of crystallinity ranged from 31.94 to 34.06%. The phosphorus content ranged from 0.003 to 0.011%, the amylose ranged from 25.13 to 29.01% and the resistant starch ranged from 65.70 to 80.28%. The starches showed high peak viscosity and breakdown, especially those obtained from 'Nanicão' and 'Grand Naine'. Peak temperature of gelatinization was around 71°C, the enthalpy change (ΔH) ranged from 9.45 to 14.73Jg(-1). The starch from 'Grand Naine' showed higher swelling power (15.19gg(-1)) and the starch from 'Prata-Anã' higher solubility (11.61%). The starches studied are highlighted by their physical and chemical characteristics and may be used in several applications.

  8. Wild Musa Species Collection of Purwodadi Botanic Garden: Inventory and Its Morpho - taxonomic Review

    Directory of Open Access Journals (Sweden)

    Lia Hapsari

    2014-01-01

    Full Text Available Indonesia, being part of the center of origin of bananas (Musaceae, has a large number diversity of bananas both wild seeded species and edible seedless cultivated varieties. Inventory of wild Musa species in Purwodadi Botanic Garden has been conducted through compiling data records from PBG’s Registration section, field inspection and observation to living collections in the garden, herbarium specimens and literature studies. The results show that total 17 wild Musa accessions has been recorded planted in Purwodadi Botanic Garden since 1990 until 2012; comprises of 8 Musa acuminata sub species, 2 Musa balbisiana forms, 1 Musa ornata, 1 Musa troglodytarum, 1 Musa borneensis and 4 unidentified species Musa spp.; but only 8 living accessions remained in 2012. Morphotaxonomic review of those 8 wild Musa accessions remained will be discussed in this paper including their geographical distributions. According to its differentiated morphological characteristics observations, it is known that there are three accessions were resembled cultivars and one unidentified species have been determined its species level, so that their registration identity needs to be revised. It is important next to prioritize ex-situ conservation of wild Musa species not yet collected in Purwodadi Botanic Garden especially from Eastern Indonesia.

  9. Expansion of banana (Musa acuminata) gene families involved in ethylene biosynthesis and signalling after lineage-specific whole-genome duplications.

    Science.gov (United States)

    Jourda, Cyril; Cardi, Céline; Mbéguié-A-Mbéguié, Didier; Bocs, Stéphanie; Garsmeur, Olivier; D'Hont, Angélique; Yahiaoui, Nabila

    2014-05-01

    Whole-genome duplications (WGDs) are widespread in plants, and three lineage-specific WGDs occurred in the banana (Musa acuminata) genome. Here, we analysed the impact of WGDs on the evolution of banana gene families involved in ethylene biosynthesis and signalling, a key pathway for banana fruit ripening. Banana ethylene pathway genes were identified using comparative genomics approaches and their duplication modes and expression profiles were analysed. Seven out of 10 banana ethylene gene families evolved through WGD and four of them (1-aminocyclopropane-1-carboxylate synthase (ACS), ethylene-insensitive 3-like (EIL), ethylene-insensitive 3-binding F-box (EBF) and ethylene response factor (ERF)) were preferentially retained. Banana orthologues of AtEIN3 and AtEIL1, two major genes for ethylene signalling in Arabidopsis, were particularly expanded. This expansion was paralleled by that of EBF genes which are responsible for control of EIL protein levels. Gene expression profiles in banana fruits suggested functional redundancy for several MaEBF and MaEIL genes derived from WGD and subfunctionalization for some of them. We propose that EIL and EBF genes were co-retained after WGD in banana to maintain balanced control of EIL protein levels and thus avoid detrimental effects of constitutive ethylene signalling. In the course of evolution, subfunctionalization was favoured to promote finer control of ethylene signalling.

  10. Image analysis to evaluate the browning degree of banana (Musa spp.) peel.

    Science.gov (United States)

    Cho, Jeong-Seok; Lee, Hyeon-Jeong; Park, Jung-Hoon; Sung, Jun-Hyung; Choi, Ji-Young; Moon, Kwang-Deog

    2016-03-01

    Image analysis was applied to examine banana peel browning. The banana samples were divided into 3 treatment groups: no treatment and normal packaging (Cont); CO2 gas exchange packaging (CO); normal packaging with an ethylene generator (ET). We confirmed that the browning of banana peels developed more quickly in the CO group than the other groups based on sensory test and enzyme assay. The G (green) and CIE L(∗), a(∗), and b(∗) values obtained from the image analysis sharply increased or decreased in the CO group. And these colour values showed high correlation coefficients (>0.9) with the sensory test results. CIE L(∗)a(∗)b(∗) values using a colorimeter also showed high correlation coefficients but comparatively lower than those of image analysis. Based on this analysis, browning of the banana occurred more quickly for CO2 gas exchange packaging, and image analysis can be used to evaluate the browning of banana peels.

  11. Pineapple juice and its fractions in enzymatic browning inhibition of banana [Musa (AAA group) Gros Michel].

    Science.gov (United States)

    Chaisakdanugull, Chitsuda; Theerakulkait, Chockchai; Wrolstad, Ronald E

    2007-05-16

    The effectiveness of pineapple juice in enzymatic browning inhibition was evaluated on the cut surface of banana slices. After storage of banana slices at 15 degrees C for 3 days, pineapple juice showed browning inhibition to a similar extent as 8 mM ascorbic acid but less than 4 mM sodium metabisulfite. Fractionation of pineapple juice by a solid-phase C18 cartridge revealed that the directly eluted fraction (DE fraction) inhibited banana polyphenol oxidase (PPO) about 100% when compared to the control. The DE fraction also showed more inhibitory effect than 8 mM ascorbic acid in enzymatic browning inhibition of banana puree during storage at 5 degrees C for 24 h. Further identification of the DE fraction by fractionation with ion exchange chromatography and confirmation using model systems indicated that malic acid and citric acid play an important role in the enzymatic browning inhibition of banana PPO.

  12. Inventory of Musa paradisiaca L. (banana kepok in Lumajang regency, Malang regency, and Magelang regency

    Directory of Open Access Journals (Sweden)

    Suhadi Suhadi

    2010-12-01

    Full Text Available Banana is fruit containing fairly high nutrition and provides quick reserve enegy. The crop grows in tropical area with average rainfall all the year and banana produces at any season. One of the bananas which has high value sale and high competable potency is subvariety of kepok banana. Kepok banana has various subvarieties, these subvarieties have the same morphologies but have different texture appearances thus uneasy to differenciate among them. The texture appearance determines the quality and price of the banana. Often the buyer makes a mistake in choosing subvariety of kepok he wants to, whereas the seller gives him the cheapiest subvariety of kepok. Methods we used was method of exploration using free exploration technique step by step without any certain path. There were two phases in the research namely the fi rst phase was carried out in field and the second phase was done in the laboratory. Subvarieties of kepok found in Lumajang Regency are 4 subcultivars, Malang Regency there are 3 and Magelang Regency are subcultivars subcultivars, The sequence of the qulity of kapok subcultivars are as follows, red kepok, yellow kepok, big (gede, gilo, gembrot kepok, and white kepok. Sugestion, organic ferlitilizer should be used in the fertilization of banana cultivation, and conservation of red kepok is highly required.

  13. High efficiency transformation of banana [Musa acuminata L. cv. Matti (AA)] for enhanced tolerance to salt and drought stress through overexpression of a peanut salinity-induced pathogenesis-related class 10 protein.

    Science.gov (United States)

    Rustagi, Anjana; Jain, Shalu; Kumar, Deepak; Shekhar, Shashi; Jain, Mukesh; Bhat, Vishnu; Sarin, Neera Bhalla

    2015-01-01

    Bananas and plantains (Musa spp. L.) are important subsistence crops and premium export commodity in several countries, and susceptible to a wide range of environmental and biotic stress conditions. Here, we report efficient, rapid, and reproducible Agrobacterium-mediated transformation and regeneration of an Indian niche cultivar of banana [M. acuminata cv. Matti (AA)]. Apical meristem-derived highly proliferative multiple shoot clump (MSC) explants were transformed with the Agrobacterium strain EHA105 harboring a binary vector pCAMBIA-1301 carrying hptII and uidA. Sequential agro-infiltration (10 min, 400 mmHg), infection (additional 35 min, Agrobacterium density A 600 = 0.8) and co-cultivation (18 h) regimen in 100 µM acetosyringone containing liquid medium were critical factors yielding high transformation efficiency (~81 %) corroborated by transient GUS expression assay. Stable transgenic events were recovered following two cycles of meristem initiation and selection on hygromycin containing medium. Histochemical GUS assay in several tissues of transgenic plants and molecular analyses confirmed stable integration and expression of transgene. The protocol described here allowed recovery of well-established putative transgenic plantlets in as little as 5 months. The transgenic banana plants could be readily acclimatized under greenhouse conditions, and were phenotypically similar to the wild-type untransformed control plants (WT). Transgenic plants overexpressing Salinity-Induced Pathogenesis-Related class 10 protein gene from Arachis hypogaea (AhSIPR10) in banana cv. Matti (AA) showed better photosynthetic efficiency and less membrane damage (P < 0.05) in the presence of NaCl and mannitol in comparison to WT plants suggesting the role of AhSIPR10 in better tolerance of salt stress and drought conditions.

  14. Species of beetles (Coleoptera; Scarabaeidae associated to banana (Musa spp. in Ceballos, Ciego de Avila, Cuba

    Directory of Open Access Journals (Sweden)

    Maria Luisa Sisne Luis

    2016-03-01

    Full Text Available A white light trap was placed in bananas plantations, according to Sisne, 2009 and MINAG, 1985, in the Citric enterprise of Ciego de Ávila during the period between May and July of 2010 with the objective of determining the composition of genus and species of the order Coleoptera family Scarabaeidae associated to the agroecosystem. The species Cyclocephala cubana Chapin, Phyllophaga puberula Duval, and Phyllophaga patruelis Chev. are associated to bananas crops in these areas.

  15. Antihypercholesterolemic and antioxidant effect of sterol rich methanol extract of stem of Musa sapientum (banana) in cholesterol fed wistar rats.

    Science.gov (United States)

    Dikshit, Piyush; Tyagi, Mool Kumar; Shukla, Kirtikar; Gambhir, Jasvindar K; Shukla, Rimi

    2016-03-01

    Musa sapientum Linn. (English 'Banana' family Musaceae), is a plant with nutritive, as well as medicinal value. Antihypercholesterolemic and antioxidant effect of methanolic extract of stem of this plant was investigated in hypercholesterolemic rats. Rats were made hypercholesterolemic by feeding cholesterol (100 mg/kg/day) suspended in soya oil. Treatment groups received extract at a dose of 10, 20 and 40 mg/kg/day in addition to cholesterol orally once daily. Fasting blood samples were collected before and after 6 weeks treatment. Animals were sacrificed and liver stored at -80 °C. Total cholesterol, HDL-cholesterol and triacylglycerol were estimated in blood. Malondialdehyde, reduced glutathione, superoxide dismutase and catalase were measured in blood and liver. Total lipids, HMG CoA redutase and lipoprotein lipase were investigated in liver. Most effective dose was found to be 20 mg/kg/day. Rise in total cholesterol, LDL + VLDL-cholesterol and triacylglycerol in animals receiving only cholesterol was 179 %, 417 % and 74 % respectively, while in animals receiving 20 mg/kg dose rise in these parameters was restricted to 40 %, 106 % and 24 %. HDL-cholesterol decreased by 12 % in extract treated group, while it decreased to 36 % in untreated hypercholesterolemic rats. Malonaldialdehyde, marker of lipid peroxidation decreased while reduced glutathione and enzymes superoxide dismutase and catalase increased significantly in blood and liver (p HMG CoA redutase and lipoprotein lipase were restored to near normal. Gas chromatography mass spectroscopy indicated high content of sterols in extract. Study demonstrated that methanol extract of stem of Musa sapientum has significant antihypercholesterolemic and antioxidant effects.

  16. Hypolipidemic Effects of Aqueous Extract of Three Cultivars of Musa sapientum Fruit Peel on Poloxamer-407 Induced Hyperlipidemic Wistar Rats.

    Directory of Open Access Journals (Sweden)

    C EDENTA

    2014-12-01

    Full Text Available Objective: The effect of aqueous extracts of the ripped fruit peel of three cultivars of Musa sapientum (Saro, Ominni and Oranta on the lipid profile of normolipidemic and hyperlipidemic rats were examined. Methods: Aqueous peel extracts of the 3-cultivars of Musa sapientum (100mg/Kg bw were administered to normolipidemic and poloxamer-407 induced hyperlidemic rats (140-180g. Atorvastatin was used as standard drug (70 mg/Kg bw. Blood samples were collected for determination of plasma total cholesterol (Tc, triacylglycerides (TAG, low-density lipoprotein cholesterol (LDL-c and high-density lipoprotein cholesterol (HDL-c after 21-days in normolipidemic rats and 3-days in hyperlipidemic rats. Results: Normolipidemic rats treated with extract of Saro showed a significant (p0.05 difference when compared to the Atorvastatin and normal control groups. Conclusion: The findings in this study reveals Oranta cultivar of M. sapientum as a better potential drug candidate among the cultivars studied, hence could be useful for the treatment of hyperlipidemia and other cardiovascular related diseases.

  17. A molecular marker-based linkage map of diploid bananas (Musa acuminata).

    Science.gov (United States)

    Fauré, S; Noyer, J L; Horry, J P; Bakry, F; Lanaud, C; Gońzalez de León, D

    1993-12-01

    A partial molecular linkage map of the Musa acuminata diploid genome is presented. This map is based on 58 RFLP, four isozyme and 28 RAPD markers segregating in an F2 population of 92 individuals. A total of 90 loci was detected, 77 of which were placed on 15 linkage groups while 13 segregated independently. Segregation distortions were shown by 36% of all loci, mostly favoring the male parent. Chromosome structural rearrangements were believed to be one of the main causes of these distortions. The use of genetic linkage data to further the genetic and evolutionary knowledge of the genus Musa, as well as to help improve the design of breeding strategies, is discussed.

  18. Soil water requirements of tissue-cultured Dwarf Cavendish banana ( Musa spp. L)

    Science.gov (United States)

    Shongwe, V. D.; Tumber, R.; Masarirambi, M. T.; Mutukumira, A. N.

    The banana is one of the most important fruit crops in the world. In terms of consumption, the banana fruit is ranked high yet there has not been much research particularly in relation to water requirements for propagules produced by tissue culture. In recent years, tissue culture banana planting material has become increasingly important due to its vigorous growth and high yields. The objective of this study was to investigate optimum soil water requirements of tissue-cultured banana. Dwarf Cavendish tissue-cultured plantlets grown in pots in a greenhouse were subjected to four irrigation regimes at 100% ETm, 85% ETm, 65% ETm, and 40% ETm. Plant parameters measured were leaf number, plant height, pseudo-stem girth, leaf length, leaf width, leaf area, leaf area index, leaf index, leaf colour, and plant vigour. Soil water potential measurements were also made over a three-month period. Differences between irrigating at 100% ETm and 85% ETm were not significantly ( P plant height, and plant height, compared to 65% and 40% ETm treatments. Pseudo-stem girth was highest from the 100% ETm compared to the other treatments. Economic yields of banana may be obtained with irrigation regimes ranging between 100% ETm and 85% ETm.

  19. Pathogenicity of 6 Populations of Radopholus similis From Ornamentals to 4 Banana Cultivars%香蕉穿孔线虫观赏植物种群对香蕉的致病性研究

    Institute of Scientific and Technical Information of China (English)

    秦丹; 谢辉; 裴艳艳; 徐春玲; 韩玉春; 黄春晓; 李云

    2009-01-01

    [目的]为了明确香蕉穿孔线虫(gadopholus similis)观赏植物种群对香蕉(Musa spp.)的致病性.[方法]通过室内盆栽接种测试的方法,研究侵入中国的6个香蕉穿孔线虫观赏植物种群对大蕉、粉蕉、皇帝蕉和巴西蕉的幼苗的致病性.[结果]供试的6个香蕉穿孔线虫观赏植物种群都能明显侵染大蕉、粉蕉和皇帝蕉,并显著抑制大蕉、粉蕉和皇帝蕉的生长;只有2个种群对巴西蕉表现为弱致病,对其生长有一定的抑制.[结论]侵入中国的6个香蕉穿孔线虫观赏植物种群普遍对大蕉、粉蕉和皇帝蕉有明显的致病性,少数种群对巴西蕉有一定的致病作用.%[Objective] The pathogenicity of 6 populations of Radopholus similis from ornamental introduced into China were tested to seedlings of four banana cultivars. [Method] Pot inoculation was used in greenhouse tests. [Result] The six populations of R. similis tested obviously infected the roots of Musa paraolisiaca, Musa ABB Pisang Awak and Musa acuminata cv. Mas and reduced their plant growth. However only two populations of R. similis were weakly infected the roots of Musa AAA Giant Cavendish cv. Baxi. [Conclusion] The results demonstrated the significantly differences in pathogenicity of 6 populations of R. similis from omamantals to seedlings of different Musa spp. The pathogenicity of 6 populations of R. similis was significantly different to the same variety of bananas, and different varieties of bananas showed different susceptibility to the same population of R. similis.

  20. Extraction of Cellulose from Kepok Banana Peel (Musa parasidiaca L. for Adsorption Procion Dye

    Directory of Open Access Journals (Sweden)

    Poedji Loekitowati Hariani

    2016-05-01

    cellulose. The morphology of cellulose more homogenous than kepok banana peel powder. It was observed that the optimum adsorption of Procion dye by cellulose was on the initial concentration of 30 mg/L, pH solution of 5 and contact time within 30 minutes. The obtained result that cellulose has removal percentage to adsorp Procion dye more higher than kepok banana peel powder. The adsorption equilibrium showed the Langmuir isotherm was described well for adsorption process (R2 = 0.991 than Freundlich isotherm (R2 = 0.922.

  1. Allometric growth relationships of East Africa highland bananas (Musa AAA-EAHB) cv. Kisansa and Mbwazirume

    NARCIS (Netherlands)

    Nyombi, K.; Asten, van P.J.A.; Leffelaar, P.A.; Corbeels, M.; Kaizzi, C.K.; Giller, K.E.

    2009-01-01

    Highland bananas are an important staple food in East Africa, but there is little information on their physiology and growth patterns. This makes it difficult to identify opportunities for yield improvement. We studied allometric relationships by evaluating different phenological stages of highland

  2. Spatial distribution of nematodes in three banana ( Musa AAA) root parts considering two root thickness in three farm management systems

    Science.gov (United States)

    Araya, M.; De Waele, D.

    2004-10-01

    The spatial location of the banana ( Musa AAA) root parasitic nematodes within three root parts considering two root thickness was determined in three commercial farm management systems, which differ in weed and nematode management. Roots in each farm management system were classified in thick (>5 mm-d) and thin (1 ≤ 5 mm-d) roots. From each root type, the epidermis, the cortical parenchyma (CP) and the vascular cylinder (VC) were separated by fingernail, and nematodes were extracted by maceration of each root part. Independent of the farm management system, and for either root thickness, highest numbers of Radopholus similis per gram of root was found in the CP, followed by the epidermis and VC. The highest number of Helicotylencus spp., Pratylenchus spp. and the total nematode population per gram of root was found in the epidermis. Considering the number of nematodes per root part, the highest number of R. similis and total nematodes was located in the CP, while Helicotylenchus spp. and Pratylenchus spp. were concentrated in the epidermis. These patterns were approximately reproduced in the two root thickness and in the three farm management systems. This behavior suggests that injection of systemic nematicides into the plant pseudostem to replace the granular applications on surface soil might be promissory.

  3. Antioxidant effcacy of unripe banana (Musa acuminata Colla peel extracts in sunflower oil during accelerated storage

    Directory of Open Access Journals (Sweden)

    Stella Sye Chee Ling

    2015-12-01

    Full Text Available Background. Sunflower oil is prone to oxidation during storage time, leading to production of toxic com- pounds that might affect human health. Synthetic antioxidants are used to prevent lipid oxidation. Spreading interest in the replacement of synthetic food antioxidants by natural ones has fostered research on fruit and vegetables for new antioxidants. Material and methods. In this study, the efficacy of unripe banana peel extracts (100, 200 and 300 ppm  in stabilizing sunflower oil was tested under accelerated storage (65°C for a period of 24 days. BHA and α-tocopherol served as comparative standards besides the control. Established parameters such as peroxide value (PV, iodine value (IV, p-anisidine value (p-AnV, total oxidation value (TOTOX, thiobarbituric acid reactive substances (TBARS and free fatty acid (FFA content were used to assess the extent of oil deterioration. Results. After 24 days storage at 65°C, sunflower oil containing 200 and 300 ppm extract of unripe banana peel showed significantly lower PV and TOTOX compared to BHA and α-tocopherol. TBARS, p-AnV and FFA values of sunflower oil containing 200 and 300 ppm of unripe banana peel extract exhibited comparable inhibitory effects with BHA. Unripe banana peel extract at 200 and 300 ppm demonstrated inhibitory effect against both primary and secondary oxidation up to 24 days under accelerated storage conditions. Conclusions. Unripe banana peel extract may be used as a potential source of natural antioxidants in the ap- plication of food industry to suppress lipid oxidation.

  4. CARBOHYDRATES, PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY IN PULP AND PEEL OF 15 BANANA CULTIVARS

    Directory of Open Access Journals (Sweden)

    CÉSAR FERNANDES AQUINO

    Full Text Available ABSTRACT The aim of this study was to quantify and compare the levels of carbohydrates and phenolic compounds and the antioxidant activity in the pulp and peel of 15 banana cultivars in two ripening stages. Four bunches per cultivar were harvested in the pre-climacteric stage, six fruits were used by sample unit. Fruits were analyzed in the pre-climacteric stage and after ripening. Total, reducing and non-reducing soluble sugars, starch, phenolic compounds and antioxidant activity were evaluated. Cultivar and ripening stage influenced all characteristics analyzed. Unripe pulp and peel had small percentage of sugar, but high percentage of starch, especially ‘Terrinha’ and ‘Marmelo’ cultivars. AAB and ABB cultivars presented the highest percentages of starch, when compared to AA and AAA cultivars. For the phenolic compounds, the highest content was observed in ripe peel, followed by ripe pulp and unripe peel and pulp, highlighting ‘Terrinha’ cultivar in all parts and stages evaluated. The antioxidant potential was higher in ripe peel, followed by unripe peel, ripe and unripe pulp. Fruits of Terrinha, Marmelo, Maçã, Ouro and Caru-Verde cultivars showed the highest carbohydrate contents, and phenolic compounds or antioxidant activity, justifying future actions in the expansion of planting and consumption of these fruits.

  5. Pyrolytic oil of banana (Musa spp.) pseudo-stem via fast process

    Energy Technology Data Exchange (ETDEWEB)

    Abdullah, Nurhayati; Sulaiman, Fauziah; Taib, Rahmad Mohd [School of Physics, Universiti Sains Malaysia, 11800 USM Pulau Pinang (Malaysia); Miskam, Muhamad Azman [Science and Engineering Research Centre, Universiti Sains Malaysia, 14300 Nibong Tebal, Pulau Pinang (Malaysia)

    2015-04-24

    This study was an attempt to produce bio-oil from banana pseudo-stem, a waste of banana cultivation, using fast pyrolysis technology. The compositions were determined and the thermal degradation behaviour of the raw material was analyzed using Perkin-Elmer Simultaneous Thermal Analyzer (STA) 6000. A 300 g/h fluidized bed bench scale fast pyrolysis unit, assembled with double screw feeders and cyclones, operating at atmospheric pressure, was used to obtain the pyrolysis liquid. The study involves the impact of the following key variables; the reactor temperature in the range of 450–650 °C, and the residence time in the range of 1.00–3.00 s. The particle size was set at 224-400 µm. The properties of the liquid product were analyzed for calorific heating value, pH value, conductivity, water and char content. The basic functional groups of the compositions were also determined using FTIR. The properties of the liquid product were compared with other wood derived bio-oil. The pyrolysis liquids derived from banana pseudo-stem were found to be in an aqueous phase.

  6. Pyrolytic oil of banana (Musa spp.) pseudo-stem via fast process

    Science.gov (United States)

    Abdullah, Nurhayati; Sulaiman, Fauziah; Taib, Rahmad Mohd; Miskam, Muhamad Azman

    2015-04-01

    This study was an attempt to produce bio-oil from banana pseudo-stem, a waste of banana cultivation, using fast pyrolysis technology. The compositions were determined and the thermal degradation behaviour of the raw material was analyzed using Perkin-Elmer Simultaneous Thermal Analyzer.(STA) 6000. A 300 g/h fluidized bed bench scale fast pyrolysis unit, assembled with double screw feeders and cyclones, operating at atmospheric pressure, was used to obtain the pyrolysis liquid. The study involves the impact of the following key variables; the reactor temperature in the range of 450-650°C, and the residence time in the range of 1.00-3.00s. The particle size was set at 224-400µm. The properties of the liquid product were analyzed for calorific heating value, pH value, conductivity, water and char content. The basic functional groups of the compositions were also determined using FTIR. The properties of the liquid product were compared with other wood derived bio-oil. The pyrolysis liquids derived from banana pseudo-stem were found to be in an aqueous phase.

  7. 抗感枯萎病香蕉的细胞结构抗性研究%Cell Structure Alteration of Banana Cultivars with Different Resistance to Fusarium Oxysporum f. sp. Cubense

    Institute of Scientific and Technical Information of China (English)

    邝瑞彬; 李春雨; 杨静; 魏岳荣; 杨乔松; 胡春华; 盛鸥; 易干军

    2013-01-01

    Fusarium wilt is a worldwide disastrous banana disease. In this study, ultrastructure observing was performed to understand the disease-resistant mechanism of cell structure alternation in banana via Foc (Fusarium Oxysporum f. sp. Cubense) inoculation treatment. Banana cultivars with different disease-resistant levels (resistant cultivars: Kangku 5, Musa AAA, and Fenza 1, Musa ABB, and susceptible cultivars: BAXI Cavendish, Musa AAA, and Guangfen 1, Musa ABB), were inoculated by dipping roots, and any change in cell structure of pseudostem was observed by using electron microscopy. Results showed that plasmolysis, decomposition and rupture of cell wall and membranes of cytoplasmic organoids were observed in the susceptible cultivars, while the breakage in the resistant cultivars was much slight. With the inoculation of Foc4, tylose and brown-like materials in vessels were observed in both types of cultivars, but thickening cuticles and suberification, and tubercles in cell wall were only appeared in the resistant cultivars. These results indicated that ultrastructure alteration of banana pseudostem might be against infection of Fusarium Oxysporum f. sp. Cubense.%  香蕉枯萎病是影响世界香蕉产业的毁灭性病害。本研究对香蕉枯萎病抗病品种抗枯5号香蕉、粉杂一号粉蕉和感病品种巴西香蕉和广粉一号粉蕉的幼苗根系接种后的球茎组织的进行了超微结构变化观察,结果表明清水对照中4个品种球茎组织的细胞形态和结构正常且完整,细胞代谢强;枯萎病原菌侵染处理下,病菌从根部受损部位侵入,穿过薄壁组织后进入维管组织,球茎木质部导管中相继出现侵填体及一些灰褐色物质,质壁分离;感病品种巴西香蕉和广粉一号粉蕉的球茎细胞壁破损断裂,细胞器肿胀变形,膜溶解呈不完整状态;抗病品种抗枯5号香蕉和粉杂一号粉蕉受害较轻,细胞器膜受损较轻,内部结构基

  8. Transgenic expression of the rice Xa21 pattern-recognition receptor in banana (Musa sp.) confers resistance to Xanthomonas campestris pv. musacearum.

    Science.gov (United States)

    Tripathi, Jaindra N; Lorenzen, Jim; Bahar, Ofir; Ronald, Pamela; Tripathi, Leena

    2014-08-01

    Banana Xanthomonas wilt (BXW), caused by the bacterium Xanthomonas campestris pv. musacearum (Xcm), is the most devastating disease of banana in east and central Africa. The spread of BXW threatens the livelihood of millions of African farmers who depend on banana for food security and income. There are no commercial chemicals, biocontrol agents or resistant cultivars available to control BXW. Here, we take advantage of the robust resistance conferred by the rice pattern-recognition receptor (PRR), XA21, to the rice pathogen Xanthomonas oryzae pv. oryzae (Xoo). We identified a set of genes required for activation of Xa21-mediated immunity (rax) that were conserved in both Xoo and Xcm. Based on the conservation, we hypothesized that intergeneric transfer of Xa21 would confer resistance to Xcm. We evaluated 25 transgenic lines of the banana cultivar 'Gonja manjaya' (AAB) using a rapid bioassay and 12 transgenic lines in the glasshouse for resistance against Xcm. About 50% of the transgenic lines showed complete resistance to Xcm in both assays. In contrast, all of the nontransgenic control plants showed severe symptoms that progressed to complete wilting. These results indicate that the constitutive expression of the rice Xa21 gene in banana results in enhanced resistance against Xcm. Furthermore, this work demonstrates the feasibility of PRR gene transfer between monocotyledonous species and provides a valuable new tool for controlling the BXW pandemic of banana, a staple food for 100 million people in east Africa.

  9. Phenylpropanoid enzymes, phenolic polymers and metabolites as chemical defenses to infection of Pratylenchus coffeae in roots of resistant and susceptible bananas (Musa spp.).

    Science.gov (United States)

    Vaganan, M Mayil; Ravi, I; Nandakumar, A; Sarumathi, S; Sundararaju, P; Mustaffa, M M

    2014-03-01

    Activity differences of the first (phenylalanine ammonia lyase, PAL) and the last (cinnamyl alcohol dehydrogenase, CAD) enzymes of phenylpropanoid pathway in the roots of resistant (Yangambi Km5 and Anaikomban) and susceptible (Nendran and Robusta) banana cultivars caused by root lesion nematode, Pratylenchus coffeae, were investigated. Also, the accumulation of phenolics and deposition of lignin polymers in cell walls in relation to resistance of the banana cultivars to the nematode were analyzed. Compared to the susceptible cultivars, the resistant cultivars had constitutively significantly higher PAL activity and total soluble and cell wall-bound phenolics than in susceptible cultivars. The resistant cultivars responded strongly to the infection of the nematode by induction of several-time higher PAL and CAD enzymes activities, soluble and wall-bound phenolics and enrichment of lignin polymers in cell wall and these biochemical parameters reached maximum at 7th day postinoculation. In addition, profiles of phenolic acid metabolites in roots of Yangambi Km5 and Nendran were analyzed by HPLC to ascertain the underlying biochemical mechanism of bananas resistance to the nematode. Identification and quantification of soluble and cell wall-bound phenolic acids showed six metabolites and only quantitative, no qualitative, differences occurred between the resistant and susceptible cvs. and between constitutive and induced contents. A very prominent increase of p-coumaric, ferulic and sinapic acids, which are precursors of monolignols of lignin, in resistant cv. was found. These constitutive and induced biochemical alterations are definitely the chemical defenses of resistant cvs. to the nematode infection.

  10. Endophytic bacterial diversity in banana 'Prata Anã' (Musa spp. roots

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    Suzane A. Souza

    2013-01-01

    Full Text Available The genetic diversity of endophytic bacteria in banana 'Prata Anã' roots was characterized. Two hundred and one endophytic bacteria were isolated, 151 of which were classified as Gram-positive and 50 as Gram-negative. No hypersensitivity response was observed in any of the isolates. The rep-PCR technique generated different molecular profiles for each primer set (REP, ERIC and BOX. Fifty readable loci were obtained and all of the fragments were polymorphic. Amplified ribosomal DNA restriction analysis (ARDRA of the isolates based on cleavage with four restriction enzymes yielded 45 polymorphic bands and no monomorphic bands. PCR amplified the nifH gene in 24 isolates. 16S rDNA sequencing of the 201 bacterial isolates yielded 102 high-quality sequences. Sequence analyses revealed that the isolates were distributed among ten bacterial genera (Agrobacterium, Aneurinibacillus, Bacillus, Enterobacter, Klebsiella, Lysinibacillus, Micrococcus, Paenibacillus, Rhizobium and Sporolactobacillus and included 15 species. The greatest number of isolates belonged to the genus Bacillus. The bacteria identified in this study may be involved in promoting growth, phosphate solubilization, biological control and nitrogen fixation in bananas.

  11. Beneficial effects of banana leaves (Musa x paradisiaca on glucose homeostasis: multiple sites of action

    Directory of Open Access Journals (Sweden)

    Virginia D. Kappel

    2013-08-01

    Full Text Available The acute effect of crude extract, n-butanol and aqueous residual fractions of Musa x paradisiaca L., Musaceae, leaves on glycemia, serum insulin secretion and glycogen content in an in vivo approach was evaluated. In addition, the in vitro effect on disaccharidases activity and albumin glycation was studied. The crude extract and fractions, n-butanol and aqueous residual, reduced glycemia and increased liver glycogen content in hyperglycemic rats, inhibited maltase activity and the formation of advanced glycation end-products in vitro. Also, a significant increase in insulin secretion and muscle glycogen content in hyperglycemic rats was observed with oral administration of the n-butanol fraction. Phytochemical analysis demonstrated the presence of rutin in crude extract and fractions of M. x paradisiaca leaves as the major compound. These beneficial effects on the regulation of glucose homeostasis observed for M. x paradisiaca leaves and the presence of rutin as the major compound indicate potential anti-diabetic properties, since previous studies have been reported that rutin can modulate glucose homeostasis.

  12. Storage at low temperature differentially affects the colour and carotenoid composition of two cultivars of banana.

    Science.gov (United States)

    Facundo, Heliofabia Virginia De Vasconcelos; Gurak, Poliana Deyse; Mercadante, Adriana Zerlotti; Lajolo, Franco Maria; Cordenunsi, Beatriz Rosana

    2015-03-01

    Different storage conditions can induce changes in the colour and carotenoid profiles and levels in some fruits. The goal of this work was to evaluate the influence of low temperature storage on the colour and carotenoid synthesis in two banana cultivars: Prata and Nanicão. For this purpose, the carotenoids from the banana pulp were determined by HPLC-DAD-MS/MS, and the colour of the banana skin was determined by a colorimeter method. Ten carotenoids were identified, of which the major carotenoids were all-trans-lutein, all-trans-α-carotene and all-trans-β-carotene in both cultivars. The effect of the low temperatures was subjected to linear regression analysis. In cv. Prata, all-trans-α-carotene and all-trans-β-carotene were significantly affected by low temperature (p0.05). The accumulation of carotenoids in this group may be because the metabolic pathways using these carotenoids were affected by storage at low temperatures. The colour of the fruits was not negatively affected by the low temperatures (p>0.05).

  13. Expression of ACO1, ERS1 and ERF1 genes in harvested bananas in relation to heat-induced defense against Colletotrichum musae.

    Science.gov (United States)

    Zhu, Xiangfei; Wang, Aiping; Zhu, Shijiang; Zhang, Lubin

    2011-09-15

    The aim of this study was to investigate the connection between heat-induced ethylene signal changes and enhanced disease resistance. Heat enhanced ripening and elevated MaACO1 expression in naturally ripened bananas (NRB), while it delayed ripening and reduced MaACO1expression in the ethephon-treated bananas (ETB). However, in both cases, heat reduced lesion sizes infected by Colletotrichum musae. This indicates that heat-induced disease resistance in bananas was independent of ripening rate. The expression of MaERS1 gene was inhibited by heat treatment in both NRB and ETB, implying that heat as a physical signal could be sensed by banana fruits through the inhibition of ethylene receptor gene expression. The intensity of MaERF1 transcript signals was elevated in heated bananas, suggesting that the enhanced accumulation of MaERF1 transcript following heat treatment could play an important role in activation of the defense system. In ETB, inhibition of JA biosynthesis by application of IBU down-regulated the expression of MaERF and significantly weakened disease resistance, suggesting involvement of endogenous JA in induction of the gene expression, which was reconfirmed by the fact that exposure to exogenous MeJA following the combination of heat plus IBU treatment restored part of the gene expression. On the other hand, in NRB, application of IBU elevated level of MaERF1 expression at 24h and enhanced disease resistance, suggesting that, when banana was not exposed to ethephon, the expression of MaERF1 gene was not JA dependent, which was verified by the fact that MeJA application did not enhance MaERF1 gene expression. In conclusion, heat-induced disease resistance in harvested bananas could involve down-regulation of MaERS1 expression and up-regulation of MaERF1 expression and JA pathway could be involved in heat activation of the defense system in bananas exposed to ethephon.

  14. Somaclonal variation in tissue culture of banana cv. 'Kluai Num Wa'[Musa (ABB group

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    Te-chato, S.

    2007-05-01

    Full Text Available Two types of cytokinins, 6-Benzyladenine (BA and/or coconut water (CW and pH of medium were studied for their effects on shoot formation, growth and somaclonal variation of banana tissue culture. Thecultures were carried out on Murashige and Skoog (MS medium for 30 days. The average shoot length (5.82 cm was obtained in liquid medium supplemented with 15% CW. While a high number of shoots at 3.8shoots/explant were obtained in liquid medium supplemented with 5 mg/l BA. For fresh weight, liquid medium supplemented with 5 mg/l BA in combination with 15% CW gave the best results (2.26 g/shoot. Anoptimum pH for promotion shoot length (5.67 cm was 5.6 whereas the lower value (pH 4 promoted a high number of shoot formation (3.89 shoots/explant. The highest fresh weight of 1.33 g/shoot was obtained onmedium adjusted pH to 8. After maintaining the shoots by successive subculturing (3-4 week-intervals on MS medium with 5 mg/l BA several morphological abnormalities were obtained. Among those, chloroticleaves (1.5% were firstly observed in the first subculture and increased to 3% in the second subculture. Moreover, a narrow leaf at 25.76% was also observed in this period. Further subculture, more somaclonalvariation, such as a thin long shoots, nodular shoots and bamboo-like leaf, appeared. Isozyme marker revealed a difference in zymogram patterns among those somaclonal variants.

  15. 5个香蕉穿孔线虫种群对香蕉的致病性测定%Determination of Pathogenicity of 5 Populations of Radopholus similis to 3 Banana Cultivars

    Institute of Scientific and Technical Information of China (English)

    符美英; 吴凤芝; 卜小莉; 王会芳; 陈绵才

    2011-01-01

    Radopholus similis is the most serious pest of banana. The pathogenicity of 5 populations of R. Similis to seedlings of 3 banana cultivars was tested by pot-inoculation in greenhouse. The results showed that all of the 5 populations of R. Similis were highly pathogenic to Musa AAA Giant Cavendish cv. Baxi. RSI population of R. Similis showed weak pathogenicity to Musa ABB Pisang Awak and the other four populations showed no pathogenicity to Musa ABB Pisang Awak. Musa acuminata cv. Mas was not the host of these 5 R. Similis populations. Musa AAA Giant Cavendish cv. Baxi plants parasitized with R. Similis showed a reduced growth with yellowing leaves and browning spot in roots.%香蕉穿孔线虫对香蕉生产是一种毁灭性的危害.为了明确5个香蕉穿孔线虫种群对香蕉的致病性,在温室中采用盆栽接种的方法,分别测定了5个香蕉穿孔线虫花卉种群对巴西蕉、皇帝蕉和粉蕉的寄生性.结果表明,5个香蕉穿孔线虫种群对巴西蕉都有强致病性;RS1种群对粉蕉存在弱致病性,其他4个种群对粉蕉均没有致病性;皇帝蕉不是这5个香蕉穿孔线虫种群的寄主植物.香蕉穿孔线虫寄生巴西蕉后,造成香蕉植株明显矮小、长势弱,严重时地上部叶片出现萎蔫黄化,根部形成褐色病斑甚至坏死变黑腐烂等症状,对巴西蕉危害显著.

  16. From crossbreeding to biotechnology-facilitated improvement of banana and plantain.

    Science.gov (United States)

    Ortiz, Rodomiro; Swennen, Rony

    2014-01-01

    The annual harvest of banana and plantain (Musa spp.) is approximately 145 million tons worldwide. About 85% of this global production comes from small plots and kitchen or backyard gardens from the developing world, and only 15% goes to the export trade. Musa acuminata and Musa balbisiana are the ancestors of several hundreds of parthenocarpic Musa diploid and polyploid cultivars, which show multiple origins through inter- and intra-specific hybridizations from these two wild diploid species. Generating hybrids combining host plant resistance to pathogens and pests, short growth cycles and height, high fruit yield, parthenocarpy, and desired quality from the cultivars remains a challenge for Musa crossbreeding, which started about one century ago in Trinidad. The success of Musa crossbreeding depends on the production of true hybrid seeds in a crop known for its high levels of female sterility, particularly among polyploid cultivars. All banana export cultivars grown today are, however, selections from somatic mutants of the group Cavendish and have a very narrow genetic base, while smallholders in sub-Saharan Africa, tropical Asia and Latin America use some bred-hybrids (mostly cooking types). Musa improvement goals need to shift to address emerging threats because of the changing climate. Innovative cell and molecular biology tools have the potential to enhance the pace and efficiency of genetic improvement in Musa. Micro-propagation has been successful for high throughput of clean planting materials while in vitro seed germination assists in obtaining seedlings after inter-specific and across ploidy hybridization. Flow cytometry protocols are used for checking ploidy among genebank accessions and breeding materials. DNA markers, the genetic maps based on them, and the recent sequencing of the banana genome offer means for gaining more insights in the genetics of the crops and to identifying genes that could lead to accelerating Musa betterment. Likewise, DNA

  17. Histology of Callogenesis in Diploid Bananas (Musa acuminata, AA Group �Kluai Sa� and �Kluai Leb Mu Nang�

    Directory of Open Access Journals (Sweden)

    Kamnoon KANCHANAPOOM

    2012-02-01

    Full Text Available Yellow compact calluses were induced from in vitro-grown shoot tips of diploid bananas (Musa acuminata, AA group �Kluai Sa� and �Kluai Leb Mu Nang� on a modified Murashige and Skoog (MS medium containing 100 mg/L malt extract, 50 mg/L proline, 50 mg/L cysteine, 100 mg/L glutamine, 1 mg/L biotin, 7 mg/L Dicamba and 2 mg/L TDZ. Green shoot buds were induced after transfer of the yellow compact calluses to the same MS medium but supplemented with 1 mg/L NAA and 3 mg/L BA and plant regeneration was achieved through organogenesis in callus cultures. Regenerated shoots were rooted on MS medium containing 0.2% activated charcoal but without plant growth regulators. Histological analysis revealed that calluses originated from small dense cells with well stained cytoplasm and nucleus typical of meristematic cells.

  18. Ploidy Variation in Hybrids from Interploid 3x X 2x Crosses in Musa

    OpenAIRE

    Osuji, JO.; Vuylsteke, D.; Ortiz, R.

    1997-01-01

    Hybrids were obtained after in vitro germination of embryos from interploid crosses between triploid 'French' plantain cultivars (Musa spp. AAB group) 'Ntanga 2' and 'Bobby Tannap' with diploid banana (Ivlusa acuminata subsp. burmannicoidesj 'Calcutta 4'. Cross-pollinated bunches were harvested at full maturity and ripened with acetylene in a room for 4 days. Seeds were extracted from peeled ripe fruits by squashing. Embryos from the seeds were excised aseptically after 2 days and germinated ...

  19. Drying kinetics of bananas by natural convection: Influence of temperature, shape, blanching and cultivar Cinética de secagem de bananas por convecção natural: Influência da temperatura, formato, branqueamento e cultivar

    Directory of Open Access Journals (Sweden)

    Soraia Vilela Borges

    2011-04-01

    Full Text Available The influence of variables as temperature, solid shape, temperature, branching and cultivar on drying of bananas were studied. Bananas from cv. Prata and D'água, on disk and cylindrical shape, blanched or not, were dehydrated in a tray dryer in natural convection at 40 and 70ºC. Drying behavior was analyzed by using a mathematical model. The exponential model showed good agreement to experimental data (r² 0.93 - 0.99 and standard error: 0.01- 0.05. Temperature presented positive influence on drying rate in all the tested conditions. With respect to the sample shape, the disk shape carried out to significantly higher drying rates only for D'água cultivar without blanching. Blanching was significantly influent, with positive influence, on drying rate of banana cv. Prata at 40º C. The influence of the cultivar did not present a defined tendency.No presente trabalho, estudou-se a influência de variáveis como temperatura, formato do sólido, branqueamento e cultivar na secagem de bananas. Bananas cultivares Prata e D'água, nos formatos disco e cilindro, branqueadas ou não, foram desidratadas em secador de bandejas com convecção natural nas temperaturas de 40 e 70ºC. O comportamento da secagem foi analisado utilizandose modelagem matemática. O modelo exponencial mostrou bom ajuste aos resultados experimentais (r²: 0,93 - 0,99 e erro de estimativa padrão: 0,01 - 0,04. A temperatura apresentou influência positiva na taxa de secagem em todas as condições testadas. Com relação ao formato das amostras, o formato disco conduziu a taxas de secagem significativamente maiores somente para o cultivar D'água e sem branqueamento. O branqueamento se mostrou significativamente influente, com influência positiva na taxa de secagem da banana Prata a 40º C. A influência da cultivar não apresentou uma tendência definida.

  20. Granules morphology and rheological behavior of green banana (Musa cavendishii and corn (Zea mays starch gels Morfologia dos grânulos e comportamento reológico dos géis de amido de banana verde (Musa cavendishii e milho (Zea mays

    Directory of Open Access Journals (Sweden)

    Dayane Rosalyn Izidoro

    2007-10-01

    Full Text Available In this work, it was used starch obtained from green banana (Musa cavendishii and commercial corn (Zea mays starch in order to compare the granule morphology and the rheological behavior of these gel-starches. Images of starch granules morphology were obtained from scanning electron microscope (SEM. The banana starch granules presented an oval and ellipsoidal shape with irregular diameters. Neverthless, the granules of corn starch showed a poliedric shape, with different sizes. The rheological behavior of gel starch solutions showed a non-newtonian character with a pseudoplastic behavior. Herschel-Bulkley model gave a good description on the rheological behavior of the gel starch. Banana gel-starch solutions showed higher values of shear stress and apparent viscosity when compared with corn gel-starch solutions. A progressive decrease in shear stress and viscosity occurred with the addition of sodium chloride and sucrose.No presente trabalho foi utilizado amido obtido de banana verde (Musa cavendishii e amido de milho (Zea mays comercial, com o objetivo de comparar a morfologia dos grânulos e o comportamento reológico dos géis. As imagens da morfologia dos grânulos foram obtidas por microscopia eletrônica de varredura. Os grânulos de amido da banana apresentaram forma oval e elipsoidal com diâmetros irregulares, todavia, os grânulos do amido de milho mostraram forma poliédrica e diferentes tamanhos. As análises reológicas dos géis das soluções de amido mostraram caráter não-newtoniano, pseudoplástico. O modelo de Herschel-Bulkley foi o que melhor representou comportamento reológico dos géis. O gel de amido de banana verde obteve maiores valores de tensão de cisalhamento e viscosidade aparente quando comparada ao gel de amido de milho. Ocorreu um decréscimo progressivo na tensão de cisalhamento e na viscosidade com a adição de cloreto de sódio e sacarose aos géis.

  1. MACROPROPAGATION OF PLANTAIN (MUSA SPP. CULTIVARS PITA 3, FHIA 21, ORISHELE AND CORNE 1: EFFECT OF BENZYLAMINOPURINE (BAP CONCENTRATION

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    Deless Edmond Fulgence THIEMELE

    2015-12-01

    Full Text Available In Africa, plantain is one of the most important starchy food and cash crops. Nonetheless, one of the major constraints for its production was the unavailability of healthy planting materials at planting time. This constraint could be lifted using the cloning of planting materials via the in vitro micropropagation or in vivo macropropagation techniques. Shelled corms from four cultivars, known as PITA 3, FHIA 21, ORISHELE and CORNE 1, were used. Three treatments differing in three hormonal concentrations, especially 20.0, 30.0 and 40.0 mg L-1 were tested. The control one was hormone free. Tested treatments were laid out in a split plot design. The decorticated banana corms were sprayed twofold at 2 weeks interval with BAP solution when placed in sterilized soil in high humidity plastic tunnel. It emerged from results, regarding BAP concentration effect, that BAP treatment with 40 mg L-1 significantly reduced the emergence time of shoots at 20 days as against 25.1, 28.3 and 28.5 for the 2 tested other treatments as well as control, respectively. Likewise, the concentrations 40.0 mg L-1 both recorded the largest number of sprouted buds per corm and number of shoots per corm. With respect to banana cultivar effect, PITA 3 showed the largest number of shoots per corm. Basing on such findings, it is concluded that MSD technique combined with BAP at 40.0 mg L-1 is a suitable technique for improving of the in vivo macropropagation of plantain. This concentration increased at least 50 % of sucker production compared to control.

  2. Detection of antimicrobial activity of banana peel (Musa paradisiaca L. on Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans: An in vitro study

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    Suraj Premal Kapadia

    2015-01-01

    Full Text Available Introduction and Aim: Banana is used widely because of its nutritional values. In past, there are studies that show banana plant parts, and their fruits can be used to treat the human diseases. Banana peel is a part of banana fruit that also has the antibacterial activity against microorganisms but has not been studied extensively. Since, there are no studies that relate the antibacterial activity of banana peel against periodontal pathogens. Hence, the aim of this study is to determine the antimicrobial activity of banana peel extract on Porphyromonas gingivalis (P. gingivalis and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans. Material and Methods: Standard strains of P. gingivalis and A. actinomycetemcomitans were used in this study which was obtained from the in-house bacterial bank of Department of Molecular Biology and Immunology at Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre. The banana peel extract was prepared, and the antibacterial activity was assessed using well agar diffusion method and minimum inhibitory concentration was assessed using serial broth dilution method. Results: In the current study, both the tested microorganisms showed antibacterial activity. In well diffusion method, P. gingivalis and A. actinomycetemcomitans showed 15 mm and 12 mm inhibition zone against an alcoholic extract of banana peel, respectively. In serial broth dilution method P. gingivalis and A. actinomycetemcomitans were sensitive until 31.25 μg/ml dilutions. Conclusion: From results of the study, it is suggested that an alcoholic extract of banana peel has antimicrobial activity against P. gingivalis and A. actinomycetemcomitans.

  3. Development of Agrobacterium-mediated transformation of highly valued hill banana cultivar Virupakshi (AAB) for resistance to BBTV disease.

    Science.gov (United States)

    Elayabalan, Sivalingam; Kalaiponmani, Kalaimughilan; Subramaniam, Sreeramanan; Selvarajan, Ramasamy; Panchanathan, Radha; Muthuvelayoutham, Ramlatha; Kumar, Krish K; Balasubramanian, Ponnuswami

    2013-04-01

    One of the most severe viral diseases of hill banana is caused by banana bunchy top virus (BBTV), a nanovirus transmitted by the aphid Pentalonia nigronervosa. In this study, we reported the Agrobacterium-mediated transformation on a highly valued hill banana cultivar Virupakshi (AAB) for resistance to BBTV disease. The target of the RNA interference (RNAi) is the rep gene, encoded by the BBTV-DNA1. In order to develop RNAi construct targeting the BBTV rep gene, the full-length rep gene of 870 bp was polymerase chain reaction amplified from BBTV infected hill banana sample DNA, cloned and confirmed by DNA sequencing. The partial rep gene fragment was cloned in sense and anti sense orientation in the RNAi intermediate vector, pSTARLING-A. After cloning in pSTARLING-A, the cloned RNAi gene cassette was released by NotI enzyme digestion and cloned into the NotI site of binary vector, pART27. Two different explants, embryogenic cells and embryogenic cell suspension derived microcalli were used for co-cultivation. Selection was done in presence of 100 mg/L kanamycin. In total, 143 putative transgenic hill banana lines were generated and established in green house condition. The presence of the transgenes was confirmed in the selected putative transgenic hill banana lines by PCR and reverse transcription PCR analyses. Transgenic hill banana plants expressing RNAi-BBTV rep were obtained and shown to resist infection by BBTV. The transformed plants are symptomless, and the replication of challenge BBTV almost completely suppressed. Hence, the RNAi mediating resistances were shown to be effective management of BBTV in hill banana.

  4. ASPECTOS ETIOLÓGICOS DA OCORRÊNCIA DA SECA DO CACHO DA BANANEIRA PRATA (Musa sp. AAB NO ESTADO DE GOIÁS ETHIOLOGICAL ASPECTS OF BANANA BUNCH DRYING OCCURRENCE IN Musa sp. AAB Cv. PRATA IN THE GOIÁS STATE, BRAZIL

    Directory of Open Access Journals (Sweden)

    Lincoln F. Zica

    2007-09-01

    Full Text Available

    No presente trabalho procurou-se determinar a causa da “seca do cacho” de banana do cultivar Prata (Musa sp. AAB e analisar alguns aspectos de sua ocorrência no Estado de Goiás. A doença afeta drasticamente a produção, e ocorre mais severamente durante o período chuvoso (outubro - abril, podendo, em certas regiões, afetar quase todos os cachos. Mediante os resultados obtidos em isolamentos sob condições axênicas e através da aplicação do postulado de Koch, determinou-se que a causa primária da doença á o fungo Gloeosporium musarum Cooke & Massee, atuando sob condições climáticas predisponentes. Foi sugerido que características clonais, condições climáticas e potencial de inóculo disponível sejam os principais fatores envolvidos na distribuição regional da enfermidade.

    The purpose of the present study was to determine the cause of the drying of the bunch in the “Prata banana” (Musa sp. AAB and to analyze some of the aspects of its occurrence in the State of Goiás. The disease drastically affects production, and occurs more severely during the rainy season (October to April , going so far as to affect almost all of the bunches in certain regions. Based on the results obtained in isolation, under aseptic conditions, and using Koch’s postulate, the primary cause of the disease was determined to be the fungus Gloesporium musarum Cooke & Massee, which acts under predisposing climatic conditions. It was suggested that clonal characteristics, climatic conditions, and the potential of available innoculum were the principal factors involved in the spread of the disease in certain regions.

  5. ISOLATION AND IDENTIFICATION OF Streptomyces sp. ON RHIZOSPHERE PLANT BANANA (Musa paradiasica IN PENDEM VILLAGE JEMBRANA REGENCY BALI

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    Retno Kawuri

    2016-09-01

    Full Text Available Pendem village in Jembrana regency is one of the banana plantation in Bali. Now a days banana plants were attack by bacterial wilt disease with the symptoms of wilting plants, brown spots on the vessel banana stems and fruit to rot and dry. Control of use of chemical fertilizers can cause bad impact on environment and also can not control the disease. Streptomyces bacteria are bacteria that are capable of producing enzymes and antibiotics that can be used as biocontrol agents of several diseases in plants. The purpose of this research is to isolate and identify the bacteria Streptomyces from rhizosphere of banana plants without symptoms in the village Pendem Jembrana regency. The method of isolation of Streptomyces using Platting method, Streptomyces isolated from soil rhizosphere of banana plants without symptoms or health plant. Soil was taken by digging near rooting bananas plant about 15 cm from the ground and and the sample was growth on media Humic Vitamin Agar (HVA and Yeast Extract Malt Agar (ISP4. Identification macros-copically and microscopically and biochemical test using determination key book guide to the Classification and Identification of the Actinomycetes and Their antibiotics of Lechevalier and Waksman (1973. Result showed it was found 9 Streptomyces isolate; Streptomyces sp.1, Streptomyces sp. 2, Streptomyces sp.3, sp.4 Streptomyces, Streptomyces sp.5 sp.6, Streptomyces sp 7, Streptomyces sp.8 and Streptomyces sp.9. Nine isolates of Streptomyces sp. will be tested against the bacteria Ralstonia solanacearum ,the bacteria that causes bacterial wilt disease.

  6. EVALUATION OF GASTRIC ANTIULCEROGENIC ACTION OF PLANTAIN BANANA ( MUSA SAPIENTUM VAR. PARADISIACA IN ASPIRIN PLUS PYLORUS LIGATED ALBINO RATS

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    K.R. Tandel* and B.K. Shah

    2012-11-01

    Full Text Available Objectives: To assess the effect of unripe dried banana powder in experimentally induced gastric ulcers and effect on gastric acid secretion. To evaluate the antiulcerogenic effect of plantain banana of Gujarat as a part of evaluation of impact of biological variables on this activity. Materials and methods: Total of 24 albino rats of either sex weighing between 150-250 gm were randomly divided into 4 groups. Each group has 6 no. of rats. The first group received placebo (distilled water, the second, third & forth group received 0.5gm/kg, 1gm/kg and 2mg/kg of banana powder respectively. Banana powder was given as suspension at fixed time (3 times in a day for two days and animals were kept for fasting for another 48hrs. On 5th day, the animals were sacrificed after 7 hrs and stomach were removed for examination and gastric juice samples were collected to analyze volume and acidity.Results: Orally administered banana powder in the dose of 2gm/kg caused a statistically significant decrease in aspirin with pyloric ligation induced ulcers in rats without significantly decrease in secretary activity.Conclusion: It can be concluded from these results that vegetable plantain banana has antiulcerogenic and mucosal protective actions, but it has no antisecretory activity.

  7. Effects of N(6)-benzylaminopurine and Indole Acetic Acid on In Vitro Shoot Multiplication, Nodule-like Meristem Proliferation and Plant Regeneration of Malaysian Bananas (Musa spp.).

    Science.gov (United States)

    Sipen, Philip; Davey, Michael R

    2012-12-01

    Different concentrations of N(6)-benzylaminopurine (BAP) and indole acetic acid (IAA) in Murashige and Skoog based medium were assessed for their effects on shoot multiplication, nodule-like meristem proliferation and plant regeneration of the Malaysian banana cultivars Pisang Mas, Pisang Nangka, Pisang Berangan and Pisang Awak. BAP at 1-14 mg L(-1) with or without 0.2 mg L(-1) IAA, or BAP at 7-14 mg L(-1) with the same concentration of IAA, was evaluated for shoot multiplication from shoot tips and the proliferation of nodule-like meristems from scalps, respectively. Plant regeneration from scalps was assessed using 1 mg L(-1) BAP and 0.2 mg L(-1) IAA separately, or a combination of these two growth regulators. Data on shoot multiplication, the proliferation of nodule-like meristems with associated plant regeneration were recorded after 30 days of culture. A maximum of 5 shoots per original shoot tip was achieved on medium supplemented with BAP at 5 mg L(-1) (Pisang Nangka), 6 mg L(-1) (Pisang Mas and Pisang Berangan), or 7 mg L(-1) (Pisang Awak), with 0.2 mg L(-1) IAA. BAP at 11 mg L(-1) with 0.2 mg L(-1) IAA induced the most highly proliferating nodule-like meristems in the four banana cultivars. Plant regeneration from scalps was optimum in all cases on medium containing 1 mg L(-1) BAP and 0.2 mg L(-1) IAA. This is the first report on the successful induction of highly proliferating nodule-like meristems and plant regeneration from scalps of the Malaysian banana cultivars Pisang Mas, Pisang Nangka, Pisang Berangan and Pisang Awak.

  8. Prototheca associated with banana.

    Science.gov (United States)

    Pore, R S

    1985-06-01

    Prototheca stagnora was found to be a habitant of older harvested banana (Musa sapientum) and plantain (M. paradisiaca) stumps while P. wickerhamii colonized fresh Musa sp. stumps and flower bract water of Heliconia sp. While Prototheca sp. were known to habituate woody plants, this is the first evidence that herbaceous plants also serve as habitats.

  9. Technological use of green banana flour with shell (Musa AAB) as a fat susbtitute for meat models

    OpenAIRE

    Yorleny Araya-Quesada; Alejandra Morales-Torres; Lea Wexler; Pedro Vargas-Aguilar

    2014-01-01

    Green banana flour with shell was evaluated as fat substitute for meat products. Unripe plantains were dried in a hot air dryer (70 °C) and milled by a hammer mill to obtain two flours with different mesh sizes (HF, HG). Proximal composition, water holding capacity (WHC), solubility (S), swelling (SW), and fat absorption capacity (FAC) were determined. 2%, 4% and 6% of HG and HF were added to a meat model emulsion as fat replacement. The effect of adding green banana flour was evaluated on co...

  10. [In vitro activity of different fungicides on the growth in Mycosphaerella fijiensis var. difformis Stover and Dickson, Cladosporium musae Morelet and Deightoniella torulosa (Syd.) Ellis, isolated parasites of the banana phyllosphere in the Ivory Coast].

    Science.gov (United States)

    Koné, Daouda; Badou, Odjochoumou Jean; Bomisso, Edson Lezin; Camara, Brahima; Ake, Séverin

    2009-05-01

    In Côte-d'Ivoire, banana leaf surfaces are attacked by Mycosphaerella fijiensis var. difformis, Cladosporium musae, and Deigthonielle torulosa. Control is based on fungicides recommanded for Mycosphaerella fijiensis. Fungicides belonging to triazoles and strobilurines types were added, at different concentrations, to the PDA medium, using this PDA medium containing no fungicide as the control. Mycelium disc and spores of Cladosporium musae, Mycosphaerella fijiensis and Deightoniella torulosa were put on the different media. Total inhibition of mycelium growth of every fungus on the PDA amended with propiconazole was observed. The fungicides used show different activities according to their concentration and their mode of action. The application of a fungicide should talke into account the pathogenic fungus involved in the leaf attacked. Against Mycosphaerella fijiensis var. difformis, the Ci(50) are different according to the concentrations (0.44 to 1.06 ppm). Correlations ranked from 0.71 to 0.91 are also different according to fungicide used. The Ci(50) of Cladosporium musae (0.10 to 2.44 ppm) and Deightoniella torulosa (0.26 to 0.52 ppm) are different and their correlations are respectively 0.78 to 0.99 and 0.86 to 0.95. An assessment of the sensitivity of parasitic fungi of banana can be made by mycelium growth measurement of fungi isolated from resistance zones.

  11. Micromicetos del suelo de una plantación de plátano (Musa paradisiaca en Teapa, Tabasco, México Soil microfungi from a banana (Musa paradisiaca plantation in Teapa, Tabasco, Mexico

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    Mariana Del Olmo-Ruiz

    2010-04-01

    Full Text Available Se analizó la micobiota presente en el suelo de un cultivo de plátano (Musa paradisiaca L. en el municipio de Teapa en el estado de Tabasco, México. Los objetivos fueron conocer las especies habitantes de este suelo y la dinámica de la comunidad con respecto a la composición de especies a lo largo de un año. Se llevaron a cabo 4 muestreos durante temporadas distintas y los aislamientos se realizaron mediante la técnica de dilución en placa. Se recuperaron 91 colonias, correspondientes a 30 especies diferentes de hongos mitospóricos, de las cuales únicamente Aspergillus flavus Link, Fusarium nivale (Fr. Ces y Trichoderma harzianum Rifai fueron especies residentes de ese suelo y el resto, esporádicas. El índice de similitud de Sorensen evidenció una sucesión de especies entre las temporadas de muestreo. Del total de especies determinadas en este estudio, 8 no habían sido encontradas como habitantes de suelos mexicanos. Asimismo, se mostró que el suelo analizado es un hábitat muy rico en micromicetos y que es necesario incrementar los esfuerzos de colecta para tener un mayor conocimiento sobre la diversidad en nuestro país.In this study, we analyzed the soil microfungal community from a banana (Musa paradisiaca L. plantation in Teapa, a municipality of Tabasco State in Mexico. The objectives were to determine the fungal species present and to analyze the community composition throughout an entire year. We performed 4 samplings during different seasons and the fungal isolates were recovered using the dilution plate technique. We isolated 91 strains from 30 different mitosporic species where only Aspergillus flavus Link, Fusarium nivale (Fr. Ces and Trichoderma harzianum Rifai were considered as resident species while the remaining species were sporadic. The Sorensen similarity index suggested that species succession had occurred among the sampling seasons. From all the species identified in this survey, 8 had not been found as

  12. Differentiation between cooking bananas and dessert bananas. 1. Morphological and compositional characterization of cultivated Colombian Musaceae (Musa sp.) in relation to consumer preferences.

    Science.gov (United States)

    Gibert, Olivier; Dufour, Dominique; Giraldo, Andrés; Sánchez, Teresa; Reynes, Max; Pain, Jean-Pierre; González, Alonso; Fernández, Alejandro; Díaz, Alberto

    2009-09-09

    The morphological, physical, and chemical characteristics of 23 unripe cultivated varieties of Colombian Musaceae were assessed. Fresh pulp dry matter helped to discriminate the following consumption subgroups: FHIA dessert hybrids (hydes: 24.6%) plantains (pl: 41.1%). Banana flour starch content on dry basis (db) varied from 74.2 to 88.2% among the varieties, with: pl: 86.5% > cook and hycook: 84% > des: 81.9% > hydes: 79.7% (p plantain subgroup (5.6), which also had lower titratable acidity than those of the cooking banana and FHIA groups with 7.9, 13.6, and 15.6 mEq H(+)/100 g db, respectively (p plantain subgroup in relation to stakeholder and the consumer preferences.

  13. East African highland bananas (Musa spp. AAA-EA) 'worry' more about potassium deficiency than drought stress

    NARCIS (Netherlands)

    Taulya, G.

    2013-01-01

    Drought stress, potassium (K) and nitrogen (N) deficiencies are major constraints to rain-fed East African highland banana (EAHB) production in Uganda. It was hypothesised that the reduction in fresh bunch mass and increase in dry matter (DM) allocation to corms with drought stress, K and N deficien

  14. Norms for multivariate diagnosis of nutrient imbalance in the East African highland bananas (musa spp.aaa)

    NARCIS (Netherlands)

    Wairegi, L.; Asten, van P.

    2011-01-01

    Despite low yields and soil fertility problems, fertilizer use in the East African Highland banana (AAA-EA) production is absent. High fertilizer costs increase the need for site-specific fertilizer recommendations that address deficiencies. This study aimed to derive and compare norms for AAA-EA ba

  15. Aceitação de cultivares de bananas resistentes à Sigatoka Negra junto ao consumidor da região Nordeste do Brasil Acceptance of banana cultivars resistant to Black Sigatoka by the consumer market of Northeast Brazilian region

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    Deborah dos Santos Garruti

    2012-05-01

    Full Text Available O Mal da Sigatoka Negra é devastador para as cultivares tradicionais de banana. No Brasil, cultivares resistentes estão sendo desenvolvidas, mas se a preferência do consumidor não for considerada, o programa de melhoramento pode falhar. Neste trabalho, a aceitabilidade de quatro cultivares resistentes foi acessada no Nordeste brasileiro e comparada a quatro variedades comerciais. Dois grupos de consumidores participaram: adultos jovens e donas de casa. Escala hedônica foi aplicada para aparência externa dos cachos, aceitação global e aceitação por atributos dos frutos (aparência sem a casca, aroma, sabor e textura, além de testes de preferência e intenção de compra. Os dados foram submetidos à ANOVA e Mapa de Preferência Interno. Mulheres e jovens mostraram opiniões semelhantes. As cultivares 'Preciosa' e 'Pacovan Ken' mostraram ser as mais promissoras, sendo que a primeira já pode substituir as cultivares comerciais 'Prata' e 'Pacovan' sem prejudicar a sua aceitabilidade, mas a 'Pacovan Ken' ainda precisa ser melhorada quanto à aparência.The Black Sigatoka Disease is devastating to traditional banana cultivars. In Brazil, resistant cultivars are being developed but if the consumer's preference is not taken into account, the breeding program may not succeed. In this research the acceptability of four resistant cultivars was accessed in the Northeastern Brazilian region and compared to four commercial varieties. Two groups of consumers participated: young adults and housewives. Hedonic scale was applied to external appearance of bunches, global acceptance and acceptance of fruits' attributes (appearance without peel, aroma, flavor and texture. Preference and purchase intent were also evaluated. Data were submitted to ANOVA and Preference Mapping. Women and young adults showed similar opinions. 'Preciosa' and 'Pacovan Ken' cultivars showed to be the most promising ones. 'Preciosa' can already substitute the commercial 'Prata

  16. Transgenic banana expressing Pflp gene confers enhanced resistance to Xanthomonas wilt disease.

    Science.gov (United States)

    Namukwaya, B; Tripathi, L; Tripathi, J N; Arinaitwe, G; Mukasa, S B; Tushemereirwe, W K

    2012-08-01

    Banana Xanthomonas wilt (BXW), caused by Xanthomonas campestris pv. musacearum, is one of the most important diseases of banana (Musa sp.) and currently considered as the biggest threat to banana production in Great Lakes region of East and Central Africa. The pathogen is highly contagious and its spread has endangered the livelihood of millions of farmers who rely on banana for food and income. The development of disease resistant banana cultivars remains a high priority since farmers are reluctant to employ labor-intensive disease control measures and there is no host plant resistance among banana cultivars. In this study, we demonstrate that BXW can be efficiently controlled using transgenic technology. Transgenic bananas expressing the plant ferredoxin-like protein (Pflp) gene under the regulation of the constitutive CaMV35S promoter were generated using embryogenic cell suspensions of banana. These transgenic lines were characterized by molecular analysis. After challenge with X. campestris pv. musacearum transgenic lines showed high resistance. About 67% of transgenic lines evaluated were completely resistant to BXW. These transgenic lines did not show any disease symptoms after artificial inoculation of in vitro plants under laboratory conditions as well as potted plants in the screen-house, whereas non-transgenic control plants showed severe symptoms resulting in complete wilting. This study confirms that expression of the Pflp gene in banana results in enhanced resistance to BXW. This transgenic technology can provide a timely solution to the BXW pandemic.

  17. Fusarium Wilt of Banana.

    Science.gov (United States)

    Ploetz, Randy C

    2015-12-01

    Banana (Musa spp.) is one of the world's most important fruits. In 2011, 145 million metric tons, worth an estimated $44 billion, were produced in over 130 countries. Fusarium wilt (also known as Panama disease) is one of the most destructive diseases of this crop. It devastated the 'Gros Michel'-based export trades before the mid-1900s, and threatens the Cavendish cultivars that were used to replace it; in total, the latter cultivars are now responsible for approximately 45% of all production. An overview of the disease and its causal agent, Fusarium oxysporum f. sp. cubense, is presented below. Despite a substantial positive literature on biological, chemical, or cultural measures, management is largely restricted to excluding F. oxysporum f. sp. cubense from noninfested areas and using resistant cultivars where the pathogen has established. Resistance to Fusarium wilt is poor in several breeding targets, including important dessert and cooking cultivars. Better resistance to this and other diseases is needed. The history and impact of Fusarium wilt is summarized with an emphasis on tropical race 4 (TR4), a 'Cavendish'-killing variant of the pathogen that has spread dramatically in the Eastern Hemisphere.

  18. Evolutionary Expansion of WRKY Gene Family in Banana and Its Expression Profile during the Infection of Root Lesion Nematode, Pratylenchus coffeae

    Science.gov (United States)

    Suthanthiram, Backiyarani; Subbaraya, Uma; Marimuthu Somasundram, Saraswathi; Muthu, Mayilvaganan

    2016-01-01

    The WRKY family of transcription factors orchestrate the reprogrammed expression of the complex network of defense genes at various biotic and abiotic stresses. Within the last 96 million years, three rounds of Musa polyploidization events had occurred from selective pressure causing duplication of MusaWRKYs with new activities. Here, we identified a total of 153 WRKY transcription factors available from the DH Pahang genome. Based on their phylogenetic relationship, the MusaWRKYs available with complete gene sequence were classified into the seven common WRKY sub-groups. Synteny analyses data revealed paralogous relationships, with 17 MusaWRKY gene pairs originating from the duplication events that had occurred within the Musa lineage. We also found 15 other MusaWRKY gene pairs originating from much older duplication events that had occurred along Arecales and Poales lineage of commelinids. Based on the synonymous and nonsynonymous substitution rates, the fate of duplicated MusaWRKY genes was predicted to have undergone sub-functionalization in which the duplicated gene copies retain a subset of the ancestral gene function. Also, to understand the regulatory roles of MusaWRKY during a biotic stress, Illumina sequencing was performed on resistant and susceptible cultivars during the infection of root lesion nematode, Pratylenchus coffeae. The differential WRKY gene expression analysis in nematode resistant and susceptible cultivars during challenged and unchallenged conditions had distinguished: 1) MusaWRKYs participating in general banana defense mechanism against P.coffeae common to both susceptible and resistant cultivars, 2) MusaWRKYs that may aid in the pathogen survival as suppressors of plant triggered immunity, 3) MusaWRKYs that may aid in the host defense as activators of plant triggered immunity and 4) cultivar specific MusaWRKY regulation. Mainly, MusaWRKY52, -69 and -92 are found to be P.coffeae specific and can act as activators or repressors in a

  19. Características pós-colheita de frutos de genótipos de bananeira (Musa spp. Post-harvest fruit characteristics of different banana (Musa spp. genotypes

    Directory of Open Access Journals (Sweden)

    Reginaldo Conceição Cerqueira

    2002-12-01

    Full Text Available O conhecimento das características pós-colheita de novos híbridos de bananeira é uma importante informação que auxilia o melhorista em sua tomada de decisão. Este trabalho teve como objetivo avaliar genótipos de bananeira com relação às características de pós-colheita e resistência ao desprendimento do fruto. Foram utilizados frutos da segunda penca de 16 híbridos (Calipso, Bucaneiro, Ambrosia, YB42-21, PV42-53, PV42-68, PV42-81, PV42-85, PV42-129, PV42-142, PV42-143, ST12-31, ST42-08, PV03-44, FHIA-03 e SH 3640 e quatro cultivares (Pacovan, Prata Comum, Nam e Figue Pomme Naine, que foram avaliadas quanto ao peso, comprimento, circunferência, relação polpa/casca, resistência ao desprendimento, firmeza, sólidos solúveis totais (SST, acidez total titulável (ATT, relação SST/ATT e tempo de amadurecimento. Empregou-se um delineamento inteiramente casualizado, com cinco repetições para todas as características, exceto para a firmeza do fruto em que se utilizaram dez repetições. A unidade experimental foi constituída de um único fruto. Os dados foram submetidos à analise de variância e as médias comparadas pelo Teste Scott & Knott, a 5% de probabilidade. A cultivar Nam apresentou a menor acidez titulável e a maior relação SST/ATT, enquanto a maior firmeza do fruto foi apresentada pelos híbridos PV42-81 e PV42-53. Os frutos mais pesados foram do híbrido SH 3640, e os mais compridos foram dos híbridos Bucaneiro e PV42-81. Os híbridos PV42-85 e ST42-08 apresentaram maior resistência ao desprendimento, não diferindo da 'Pacovan'. No entanto, superaram a 'Prata Comum', que obteve a menor resistência. Os genótipos 'Nam', PV42-143 e PV42-129 apresentaram maior tempo de maturação após a colheita quando comparados, principalmente, à 'Prata Comum'. A 'Nam' deve ser considerada como uma nova alternativa na produção de banana. O SH3640, híbrido da Prata Anã, pode ser recomendado como nova cultivar. Os h

  20. Micropropagation of banana.

    Science.gov (United States)

    Kaçar, Yıldız Aka; Faber, Ben

    2012-01-01

    Banana (Musa spp. AAA) is propagated vegetatively and can be rapidly and efficiently propagated by micropropagation. Conventional micropropagation techniques, however, may be too costly for commercial purposes. Our laboratory has found that depending on the combination of culture vessel and gelling agent more economic methods can be chosen for successfully micropropagating banana.

  1. Effect of Reinforcement of Hydrophobic Grade Banana (Musa ornata Bark Fiber on the Physicomechanical Properties of Isotactic Polypropylene

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    Md. Mamunur Rashid

    2016-01-01

    Full Text Available This research studied the physicomechanical as well as morphological properties of alkali treated (NaOH and KMnO4 and untreated banana bark fiber (BBF reinforced polypropylene composites. A detailed structural and morphological characterization was performed using Fourier transform infrared spectroscopy (FTIR, scanning electron microscopy (SEM, and mechanical properties testing (tensile strength, flexural strength, and microhardness. Chemical treatments improved the hydrophobic property of the fiber and it is found to be better for KMnO4 treatment. Composites with 0, 5, 10, and 15 wt.% loadings were then compared for water uptake studies and revealed that KMnO4 treated fiber composites absorb less water compared to others. KMnO4 treatment with 15% fiber loading improved the tensile strength, flexural strength, and microhardness of the composites compared to raw and NaOH treated fiber loadings. TGA analysis also shows onset temperature at 400~500°C that is associated with the decomposition of the banana fibers constituents including lignin, cellulose, and hemicelluloses which suggests better thermomechanical stability. All of the values suggest that 15% KMnO4 treated banana bark fiber (BBF/PP composites were found to be better than those of the raw and NaOH treated ones.

  2. Molecular characterization of CONSTANS-Like (COL) genes in banana (Musa acuminata L. AAA Group, cv. Grand Nain).

    Science.gov (United States)

    Chaurasia, Akhilesh Kumar; Patil, Hemant Bhagwan; Azeez, Abdul; Subramaniam, Vadakanthara Ramakrishnan; Krishna, Bal; Sane, Aniruddha Prafullachandra; Sane, Prafullachandra Vishnu

    2016-01-01

    The CONSTANS (CO) family is an important regulator of flowering in photoperiod sensitive plants. But information regarding their role in day neutral plants is limited. We report identification of nine Group I type CONSTANS-like (COL) genes of banana and their characterization for their age dependent, diurnal and tissue-specific expression. Our studies show that the Group I genes are conserved in structure to members in other plants. Expression of these genes shows a distinct circadian regulation with a peak during light period. Developmental stage specific expression reveals high level transcript accumulation of two genes, MaCOL3a and MaCOL3b, well before flowering and until the initiation of flowering. A decrease in their transcript levels after initiation of flowering is followed by an increase in transcription of other members that coincides with the continued development of the inflorescence and fruiting. CO binding cis-elements are observed in at least three FT -like genes in banana suggesting possible CO-FT interactions that might regulate flowering. Distinct tissue specific expression patterns are observed for different family members in mature leaves, apical inflorescence, bracts, fruit skin and fruit pulp suggesting possible roles other than flowering. This is the first exhaustive study of the COL genes belonging to Group I of banana.

  3. Diferenças entre bananas de cultivares Prata e Nanicão ao longo do amadurecimento: características físico-químicas e compostos voláteis Differences between Prata and Nanicão banana cultivars during ripening: physicochemical characteristics and volatile compounds

    Directory of Open Access Journals (Sweden)

    Baraquizio Braga do Nascimento Junior

    2008-09-01

    Full Text Available Bananas verdes [Nanicão (Musa sp., subgrupo Cavendish e Prata (Musa sp., subgrupo Prata] foram estudadas durante o seu amadurecimento. As propriedades físicas (firmeza, físico-químicas (pH, acidez total titulável e sólidos solúveis e químicas (açúcares, compostos fenólicos e voláteis foram analisadas e demonstraram diferenças significantes (p Fresh green bananas [Nanicão (Musa sp., subgroup Cavendish and Prata (Musa sp., subgroup Prata] were studied during their ripening. Physical (firmness, physicochemical (pH, total titrable acidity and soluble solids and chemical properties (sugars, phenolics and volatile compounds were analyzed and showed significant differences (p < 0.05. Prata presented higher values of phenolic compounds, soluble solids, sugars and firmness than Nanicão. A method to quantify the emission of esters was developed by cryogenic headspace and gas chromatography. Acetates, butyrates, isobutyrates and isovalerates were predominant. Prata produced a higher volatile concentration than Nanicão, except for acetates. In most cases, the amount of volatiles increased continuously until peel browning, followed by a decrease or a plateau.

  4. POTENSI SENYAWA POLIFENOL ANTIOKSIDAN DARI PISANG GOROHO (Musa sapien sp. The Potency of Antioxidant Polyphenol from Goroho Banana (Musa sapien Sp.

    Directory of Open Access Journals (Sweden)

    Edi Suryanto

    2012-05-01

    Full Text Available Goroho banana is food material that has positive content effect on human body. The aims of this research were toanalyze polyphenolic content and to examine antioxidant activity of goroho. Polyphenolic antioxidant of goroho was extracted using methanol, ethanol and acetone for 2 hours at room temperature. Furthermore. extract was analyzed polyphenol content, such as total phenolic, total flavonoidand condensed tannin content. To examine antioxidant activity, free radical DPPH scavenging and total antioxidant (FRAP were used by spectrophotometer analysis. Beside that, the best extract was evaluated its heat stability at 1000C for 15, 30, 45, 60 and 120 minutes.The highest total phenolic content was in acetone extract 181.87 mg/kg sample, total flavonoid content 4.75 mg/ kg sample in ethanol extract and condensed tannin 54.63 mg/kg sample in aceton extract. Acetone extract of goroho show the highest free radical scavenging activity (94.32% compare to ethanol extract (85.39 % and methanol extract (76.38 %. Although examination of total antioxidant (FRAP on aceton extract of goroho showed the highest content than methanol and ethanol extract. Total antioxidant  from acetone, methanol and ethanol extract were 1.83; 1.74; and1.74 μmol/L. This examination resulted that at 1000C there are alterations in phenolic content, free radical scavenging activity and total antioxidant after heating 120 minutes, although this three parameters alteration did not show extremedecreasing after 60 minute heating, because it still possess high activity, more than 80%. This result indicate goroho extract stability on heating depend on heating time. This results concluded that goroho possess to phenolic, flavonoid and tannin, also antioxidant activity. ABSTRAK Pisang goroho merupakan bahan makanan yang memiliki efek positif bagi kesehatan. Penelitian ini bertujuan untukmenganalisis kandungan senyawa polifenol dan menguji aktivitas antioksidan pisang goroho. Ekstraksi

  5. Expression of a ripening-related cytochrome P450 cDNA in Cavendish banana (Musa acuminata cv. Williams).

    Science.gov (United States)

    Pua, Eng-Chong; Lee, Yi-Chuan

    2003-02-13

    As part of a study to understand the molecular basis of fruit ripening, this study reports the isolation and characterization of a banana cytochrome P450 (P450) cDNA, designated as MAP450-1, which was associated with fruit ripening of banana. MAP450-1 encoded a single polypeptide of 507 amino acid residues that shared an overall identity of 27-45% with that of several plant P450s, among which MAP450-1 was most related phylogenetically to the avocado P450 CYP71A1. The polypeptide that possessed residue domains conserved in all P450s was classified as CYP71N1. Expression of CYP71N1 varied greatly between banana organs. Transcripts were detected only in peel and pulp of the ripening fruit and not in unripe fruit tissues at all developmental stages or other organs (root, leaf, ovary and flower). During ripening, transcripts were barely detectable in pre-climacteric and climacteric fruits but, as ripening progressed, they began to accumulate and reached a maximum in post-climacteric fruits. CYP71N1 expression in pre-climacteric fruit could be upregulated by exogenous application of ethylene (1-5 ppm) and treatment of overripe fruit with exogenous sucrose (50-300 mM) but not glucose downregulated the expression. These results indicate that P450s may not play a role in fruit development and its expression is associated with ripening, which may be regulated, in part, by ethylene and/or sucrose, at the transcript level.

  6. Molecular and Cytogenetic Characterization of Wild Musa Species.

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    Jana Čížková

    Full Text Available The production of bananas is threatened by rapid spreading of various diseases and adverse environmental conditions. The preservation and characterization of banana diversity is essential for the purposes of crop improvement. The world's largest banana germplasm collection maintained at the Bioversity International Transit Centre (ITC in Belgium is continuously expanded by new accessions of edible cultivars and wild species. Detailed morphological and molecular characterization of the accessions is necessary for efficient management of the collection and utilization of banana diversity. In this work, nuclear DNA content and genomic distribution of 45S and 5S rDNA were examined in 21 diploid accessions recently added to ITC collection, representing both sections of the genus Musa. 2C DNA content in the section Musa ranged from 1.217 to 1.315 pg. Species belonging to section Callimusa had 2C DNA contents ranging from 1.390 to 1.772 pg. While the number of 45S rDNA loci was conserved in the section Musa, it was highly variable in Callimusa species. 5S rRNA gene clusters were found on two to eight chromosomes per diploid cell. The accessions were genotyped using a set of 19 microsatellite markers to establish their relationships with the remaining accessions held at ITC. Genetic diversity done by SSR genotyping platform was extended by phylogenetic analysis of ITS region. ITS sequence data supported the clustering obtained by SSR analysis for most of the accessions. High level of nucleotide diversity and presence of more than two types of ITS sequences in eight wild diploids pointed to their origin by hybridization of different genotypes. This study significantly expands the number of wild Musa species where nuclear genome size and genomic distribution of rDNA loci is known. SSR genotyping identified Musa species that are closely related to the previously characterized accessions and provided data to aid in their classification. Sequence analysis of

  7. Evaluation of different protein extraction methods for banana (Musa spp.) root proteome analysis by two-dimensional electrophoresis.

    Science.gov (United States)

    Vaganan, M Mayil; Sarumathi, S; Nandakumar, A; Ravi, I; Mustaffa, M M

    2015-02-01

    Four protocols viz., the trichloroacetic acid-acetone (TCA), phenol-ammonium acetate (PAA), phenol/SDS-ammonium acetate (PSA) and trisbase-acetone (TBA) were evaluated with modifications for protein extraction from banana (Grand Naine) roots, considered as recalcitrant tissues for proteomic analysis. The two-dimensional electrophoresis (2-DE) separated proteins were compared based on protein yield, number of resolved proteins, sum of spot quantity, average spot intensity and proteins resolved in 4-7 pI range. The PAA protocol yielded more proteins (0.89 mg/g of tissues) and protein spots (584) in 2-DE gel than TCA and other protocols. Also, the PAA protocol was superior in terms of sum of total spot quantity and average spot intensity than TCA and other protocols, suggesting phenol as extractant and ammonium acetate as precipitant of proteins were the most suitable for banana rooteomics analysis by 2-DE. In addition, 1:3 ratios of root tissue to extraction buffer and overnight protein precipitation were most efficient to obtain maximum protein yield.

  8. Technological use of green banana flour with shell (Musa AAB as a fat susbtitute for meat models

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    Yorleny Araya-Quesada

    2014-12-01

    Full Text Available Green banana flour with shell was evaluated as fat substitute for meat products. Unripe plantains were dried in a hot air dryer (70 °C and milled by a hammer mill to obtain two flours with different mesh sizes (HF, HG. Proximal composition, water holding capacity (WHC, solubility (S, swelling (SW, and fat absorption capacity (FAC were determined. 2%, 4% and 6% of HG and HF were added to a meat model emulsion as fat replacement. The effect of adding green banana flour was evaluated on cooking yield, water holding capacity, pH, hardness, gumminess, chewiness, adhesiveness, elasticity, cohesiveness and color. There were not significant differences between both flours for protein, fat, ashes, WHC, S, SW, and FAC. No significant differences were found for adhesiveness, elasticity, and cohesiveness. A drop in L*, b* and C* parameters regarding the control gel was determined with the increase of fat substitution. We recommend a substitution percentage equal or lower than 4%, which represents a 20% of fat reduction.

  9. Genomic analysis of NAC transcription factors in banana (Musa acuminata) and definition of NAC orthologous groups for monocots and dicots.

    Science.gov (United States)

    Cenci, Albero; Guignon, Valentin; Roux, Nicolas; Rouard, Mathieu

    2014-05-01

    Identifying the molecular mechanisms underlying tolerance to abiotic stresses is important in crop breeding. A comprehensive understanding of the gene families associated with drought tolerance is therefore highly relevant. NAC transcription factors form a large plant-specific gene family involved in the regulation of tissue development and responses to biotic and abiotic stresses. The main goal of this study was to set up a framework of orthologous groups determined by an expert sequence comparison of NAC genes from both monocots and dicots. In order to clarify the orthologous relationships among NAC genes of different species, we performed an in-depth comparative study of four divergent taxa, in dicots and monocots, whose genomes have already been completely sequenced: Arabidopsis thaliana, Vitis vinifera, Musa acuminata and Oryza sativa. Due to independent evolution, NAC copy number is highly variable in these plant genomes. Based on an expert NAC sequence comparison, we propose forty orthologous groups of NAC sequences that were probably derived from an ancestor gene present in the most recent common ancestor of dicots and monocots. These orthologous groups provide a curated resource for large-scale protein sequence annotation of NAC transcription factors. The established orthology relationships also provide a useful reference for NAC function studies in newly sequenced genomes such as M. acuminata and other plant species.

  10. Processamento térmico de purê de banana (Musa cavendishii, Lamb. em embalagens flexíveis esterilizáveis Thermal processing of banana puree (Musa cavendishii, Lamb. in retortale pouches

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    Celso Duarte CARVALHO FILHO

    1997-12-01

    Full Text Available O objetivo deste trabalho foi estabelecer as condições de processo térmico de purê de banana, variedade "nanica", em bolsas esterilizáveis porção individual (130 x 170mm. Os frutos foram selecionados, aquecidos a 98°C/5min. para inativação enzimática, separadas as sementes e fibras num despolpador com malha de 0,08cm de diâmetro e depois o purê foi embalado nas bolsas sob um vácuo de 25mmHg, resultando em média 2,7ml de ar residual por bolsa. Em seguida as bolsas foram processadas sem agitação e em posição horizontal numa autoclave horizontal, a 115°C/158KPa, com imersão total de água quente. O tempo de manutenção do processo foi de 7,5min. Inicialmente foi utilizado o Clostridium butyricum como microrganismo alvo do processo, entretanto, observou-se que esta bactéria apresentara uma resistência térmica em purê de banana (pH 4,6 um pouco menor que o valor estimado para o Clostridium botulinum, D115=0,183min. e D115=0,236min respectivamente, sendo assim, o C. botulinum foi escolhido como microrganismo alvo da esterilização do produto. O valor de F121,1°C aplicado foi de 0,64min. para causar 12 reduções decimais, calculado segundo PFLUG (1985 para um pH de 4,6, e verificado pelo método geral. Este valor somente foi considerado para a fase de aquecimento, deixando a letalidade do resfriamento (0,34min., que foi realizada com água à temperatura ambiente, como segurança de processo. Nos testes de penetração de calor foram encontrados os valores médio de fh=6,8min; jh=0,48; fc=17,4min. e jc=1,3 demonstrando assim, que o produto é altamente condutivo. Nenhuma alteração no produto nem nas embalagens foi notada após a aplicação do ensaio de esterilidade comercial em 36 bolsas processadas contendo purê de banana, confirmando assim, a eficácia do tratamento térmico aplicado.The aim of this work was to establish thermal processing conditions for banana puree in retor pouches (130 x 170mm. The fruits were

  11. Efeito das condições de manejo nas características sensoriais de banana (Musa spp. cv. Pacovan

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    Maria Sueli Francisco

    2014-06-01

    Full Text Available Estudou-se o efeito de quatro condições de manejo sobre a aceitação de banana 'Pacovan'. Os frutos foram colhidos no ponto de maturação fisiológica ideal para o armazenamento, em quatro propriedades rurais do município de Bananeiras - PB, seguindo-se delineamento inteiramente casualizado. Os tratamentos foram identificados como: P1: irrigação+adubação química e orgânica +controle químico de plantas invasoras; P2: controle manual de plantas invasoras; P3: irrigação+adubação química+controle manual de plantas invasoras; P4: controle manual e químico de plantas invasoras. Em cada propriedade, foram colhidos cinco cachos de banana que foram submetidos à climatização com carbureto de cálcio por 12 horas para uniformização da maturação. Após esse período, foram mantidos à temperatura ambiente até sua completa maturação. O painel sensorial foi composto por 62 julgadores não treinados, com idade média de 18 anos. Os atributos aroma, sabor, textura e cor da polpa foram avaliados por meio de teste de aceitação, utilizando-se de escala hedônica estruturada de 9 pontos. Para o teste de intenção de compra, foi utilizada escala estruturada de 5 pontos. As amostras foram apresentadas aos julgadores na forma de rodelas, com espessura aproximada de 1,5 cm. Os resultados foram submetidos à análise de variância (ANOVA, e as médias, comparadas pelo teste de Tukey, ao nível de 5% de probabilidade. Para todos os atributos avaliados,os valores situaram-se entre 7,34 e 7,68, posicionando-se na escala hedônica entre os termos 'gostei moderadamente' e 'gostei muito'. Para intenção de compra, o percentual de aceitação foi de 74,19; 72,58; 72,58 e 70,97%, respectivamente, para as amostras P1, P2, P3 e P4. O tipo de manejo não influenciou na aceitação da banana 'Pacovan', sendo os frutos considerados pelos julgadores como produto de boa aceitação.

  12. Developmental localization and the role of hydroxyproline rich glycoproteins during somatic embryogenesis of banana (Musa spp. AAA

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    Menzel Diedrik

    2011-02-01

    Full Text Available Abstract Background Hydroxyproline rich glycoproteins (HRGPs are implicated to have a role in many aspects of plant growth and development but there is limited knowledge about their localization and function during somatic embryogenesis of higher plants. In this study, the localization and function of hydroxyproline rich glycoproteins in embryogenic cells (ECs and somatic embryos of banana were investigated by using immunobloting and immunocytochemistry with monoclonal JIM11 and JIM20 antibodies as well as by treatment with 3,4-dehydro-L-proline (3,4-DHP, an inhibitor of extensin biosynthesis, and by immunomodulation with the JIM11 antibody. Results Immunofluorescence labelling of JIM11 and JIM20 hydroxyproline rich glycoprotein epitopes was relatively weak in non-embryogenic cells (NECs, mainly on the edge of small cell aggregates. On the other hand, hydroxyproline rich glycoprotein epitopes were found to be enriched in early embryogenic cells as well as in various developmental stages of somatic embryos. Embryogenic cells (ECs, proembryos and globular embryos showed strong labelling of hydroxyproline rich glycoprotein epitopes, especially in their cell walls and outer surface layer, so-called extracellular matrix (ECM. This hydroxyproline rich glycoprotein signal at embryo surfaces decreased and/or fully disappeared during later developmental stages (e.g. pear-shaped and cotyledonary stages of embryos. In these later developmental embryogenic stages, however, new prominent hydroxyproline rich glycoprotein labelling appeared in tri-cellular junctions among parenchymatic cells inside these embryos. Overall immunofluorescence labelling of late stage embryos with JIM20 antibody was weaker than that of JIM11. Western blot analysis supported the above immunolocalization data. The treatment with 3,4-DHP inhibited the development of embryogenic cells and decreased the rate of embryo germination. Embryo-like structures, which developed after 3,4-DHP

  13. Efeito das condições de manejo nas características sensoriais de banana (Musa spp.) cv. Pacovan

    OpenAIRE

    2014-01-01

    Estudou-se o efeito de quatro condições de manejo sobre a aceitação de banana 'Pacovan'. Os frutos foram colhidos no ponto de maturação fisiológica ideal para o armazenamento, em quatro propriedades rurais do município de Bananeiras - PB, seguindo-se delineamento inteiramente casualizado. Os tratamentos foram identificados como: P1: irrigação+adubação química e orgânica +controle químico de plantas invasoras; P2: controle manual de plantas invasoras; P3: irrigação+adubação química+con...

  14. Isolation and characterization of a glucose/mannose-specific lectin with stimulatory effect on nitric oxide production by macrophages from the emperor banana.

    Science.gov (United States)

    Wong, Jack Ho; Ng, T B

    2006-02-01

    Emperor banana (Musa basjoo cv. 'Emperor Banana') is a banana cultivar that has not been studied previously. In this study, a glucose/mannose-specific lectin has been purified from the emperor banana by affinity chromatography on Affi-gel blue gel, ion exchange chromatography on Mono S and gel filtration by fast protein liquid chromatography on Superdex 75. This lectin was composed of two identical 15-kDa subunits with N-terminal amino acid sequence similarity to other lectins from other Musa species. Emperor banana lectin stimulated [3H-methyl]-thymidine uptake by mouse splenocytes and nitric oxide production by mouse macrophages. In contrast to Con A, the mitogenic activity of emperor banana lectin toward mouse splenocytes but not its stimulatory effect on nitric oxide production by mouse macrophages could be abrogated by 200 mM glucose. Emperor banana lectin also inhibited proliferation of leukemia cell (L1210) and the activity of HIV-1 reverse transcriptase. In summary, this is the first report of the macrophage-stimulating, antiproliferative and HIV-1 reverse transcriptase inhibiting activities of a banana lectin.

  15. Comparative biochemical analysis during the anaerobic digestion of lignocellulosic biomass from six morphological parts of Williams Cavendish banana (Triploid Musa AAA group) plants.

    Science.gov (United States)

    Kamdem, Irénée; Hiligsmann, Serge; Vanderghem, Caroline; Bilik, Igor; Paquot, Michel; Thonart, Philippe

    2013-12-01

    We studied banana lignocellulosic biomass (BALICEBIOM) that is abandoned after fruit harvesting, and assessed its biochemical methane potential, because of its potential as an energy source. We monitored biogas production from six morphological parts (MPs) of the "Williams Cavendish" banana cultivar using a modified operating procedure (KOP) using KOH. Volatile fatty acid (VFA) production was measured using high performance liquid chromatography. The bulbs, leaf sheaths, petioles-midribs, leaf blades, rachis stems, and floral stalks gave total biogas production of 256, 205, 198, 126, 253, and 221 ml g⁻¹ dry matter, respectively, and total biomethane production of 150, 141, 127, 98, 162, and 144 ml g⁻¹, respectively. The biogas production rates and yields depended on the biochemical composition of the BALICEBIOM and the ability of anaerobic microbes to access fermentable substrates. There were no significant differences between the biogas analysis results produced using KOP and gas chromatography. Acetate was the major VFA in all the MP sample culture media. The bioconversion yields for each MP were below 50 %, showing that these substrates were not fully biodegraded after 188 days. The estimated electricity that could be produced from biogas combustion after fermenting all of the BALICEBIOM produced annually by the Cameroon Development Corporation-Del Monte plantations for 188 days is approximately 10.5 × 10⁶ kW h (which would be worth 0.80-1.58 million euros in the current market). This bioenergy could serve the requirements of about 42,000 people in the region, although CH₄ productivity could be improved.

  16. Transcripts and MicroRNAs Responding to Salt Stress in Musa acuminata Colla (AAA Group cv. Berangan Roots.

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    Wan Sin Lee

    Full Text Available Physiological responses to stress are controlled by expression of a large number of genes, many of which are regulated by microRNAs. Since most banana cultivars are salt-sensitive, improved understanding of genetic regulation of salt induced stress responses in banana can support future crop management and improvement in the face of increasing soil salinity related to irrigation and climate change. In this study we focused on determining miRNA and their targets that respond to NaCl exposure and used transcriptome sequencing of RNA and small RNA from control and NaCl-treated banana roots to assemble a cultivar-specific reference transcriptome and identify orthologous and Musa-specific miRNA responding to salinity. We observed that, banana roots responded to salinity stress with changes in expression for a large number of genes (9.5% of 31,390 expressed unigenes and reduction in levels of many miRNA, including several novel miRNA and banana-specific miRNA-target pairs. Banana roots expressed a unique set of orthologous and Musa-specific miRNAs of which 59 respond to salt stress in a dose-dependent manner. Gene expression patterns of miRNA compared with those of their predicted mRNA targets indicated that a majority of the differentially expressed miRNAs were down-regulated in response to increased salinity, allowing increased expression of targets involved in diverse biological processes including stress signaling, stress defence, transport, cellular homeostasis, metabolism and other stress-related functions. This study may contribute to the understanding of gene regulation and abiotic stress response of roots and the high-throughput sequencing data sets generated may serve as important resources related to salt tolerance traits for functional genomic studies and genetic improvement in banana.

  17. 巴西香蕉苗静置水培营养液配方的初步筛选%Screening of the Nutrient Solution Formulas for Static Hydroponics of Banana (Musa AAA Cavendish Subgroup 'Brazil') Plantlets

    Institute of Scientific and Technical Information of China (English)

    黄小娟; 杨腊英; 谢德啸; 周端咏; 魏巍; 郭利佳; 黄俊生

    2012-01-01

    采用静置水培法,使用4种不同的营养液配方,并以清水培养和椰糠基质栽培为对照,对巴西香蕉苗进行生长比较试验。结果表明:在香蕉苗增加的生物量方面,BXM配方(巴西木植物营养液)处理与椰糠栽培的差异不显著,但与日本园试配方、Hoagland植物营养液配方、1/2 MS培养基营养元素配方和清水培养的差异显著;在株高和叶面积方面,均以椰糠栽培的最大,BXM配方处理的居次,接下来依次是Hoagland植物营养液配方、日本园试配方、1/2 MS培养基配方和清水培养。综上所述,在这4种营养液配方中,BXM配方是巴西香蕉苗生长的最适营养液配方。%In order to study water culture techniques, growths of the banana (Musa AAA cavendish subgroup cv. Brazil) plantlets under the static culture and substrate culture were 'compared. Four nutrient solutions including Hoagland solution, Murashige and Skoog (1/2MS) solution, BXM solution and RSYY solution were used to culture banana plantlets, which grew in tap water or in substrate culture (pearlite as substrate). The data showed that banana plantlets grew well in the four tested nutrient solutions, and there was a significant increase in biomass of the banana plantlets grew in static nutrient solution over that in tap water. Among the four nutrient solutions, the biomass of the banana plantlets that grew in BXM solution was next to that in substrate (pearlite) culture, and was significantly more than that in Hoagland solution, 1/2MS solution and RSYY solution. The indexes such as plant height and leaf area of banana plantlets that grew in BXM solution were largest among the four nutrient solutions, and then that grew in Hoagland solution, RSYY solution and MS solution reduced in turn. In conclusion, the banana plantlets grew best in BXM solution among the four nutrient solutions. So BXM solution is suggested, when growing banana plantlets in water culture.

  18. Study on levels of Kluai Hin Banana (Musa sapientum peel meal in Japanese quail ration: 2. Laying period

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    Watanasit, S.

    2005-03-01

    Full Text Available Kluai Hin banana peel meal (KHBPM contained 92.70% dry matter, 5.61% crude protein, 6.66% crude fat, 9.32% crude fiber, 10.90% ash, 60.21% NFE, 0.37% Ca, 0.22% P and 1.25% tannin on dry basis and 4,362 Kcal GE/kg. It was incorporated into 8 Japanese quail diets at 0, 5, 10, 15, 20, 25, 30 and 35%. Three hundred and thirty head of 5-weeks-old birds were randomly allotted to 8 treatments each with 4 replicates (10 quails/ replicate, fed with the above mentioned diets until 20 weeks of age. The results showed no significant (p> 0.05 difference among treatments in terms of their ages at first laying, egg production, egg weight, feed efficiency and feed consumption and feed cost when KHBPM was included up to 25% of the diet. However, treatment at the higher levels (>25% KHBPM, gave significantly (p<0.01 lower egg production and quality, feed efficiency and egg shell thickness. In addition it also gave higher feed cost (p<0.05. The average mortality rate was 0.86% and no significant difference was found among groups. Egg quality and egg yolk color-score appeared to decrease with increasing KHBPM level. From these results, it is concluded that 20- 25% KHBPM or 50.5% substation to ground corn was the optimum level for laying quails. It should be used as animal feed to reduce pollution problem.

  19. The Use of Alternate Ligno-cellulosic Raw Materials Banana (Musa sapientum Ankara (Calotropis procera and Pineapple (Ananas comosus in Handmade Paper & their Blending with Waste Paper.

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    Atul Kumar

    2013-09-01

    Full Text Available The studies were made to established suitability of lingo-cellulosic raw materials namely leaf fibreBanana (Musa Sapientum, bast fibre Ankara (Calotropis Procera, & leaf fibre Pineapple (Ananas Comosus for making pulps for handmade paper industry. This should help in providing a cost effective, good quality cellulosic raw material as an alternate to cost prohibitive traditionally used cotton hosiery waste traditionally used for manufacturing good quality handmade paper & it’s products. This will help in improving the cost economics & competitiveness of the Indian paper industry in the global market besides addressing the problems of environment & global warming. The aim of the research was to study the extraction, morphology, chemical composition and pulping of these fibres and relate these properties to the composite properties obtained with these fibres as reinforcement with short fibres viz. waste paper. For the pulp production to be feasible it is essential to use suitable pulping methods, which maximize the yield of pulp and introduce as low damage as possible to the fibres. The different pulping methods were applied to these fibre to get optimized strength properties papers. The Studies thus carried out provide useful information about the nature of these raw materials, suitable pulping & bleaching process to produce an eco-friendly handmade paper and converted products. The research work provides a good quality cost effective lingo-cellulosic raw material for handmade paper industries with a possibility of replacement of the expensive and traditionally used cotton hosiery waste.The paper thus produced using environmental friendly pulping and bleaching process is characterized for its strength properties like tensile, tear, bursting, folding endurance and other parameters. The effluents generated from pulping and bleaching of above ligno-cellulosic waste materials were characterized for various pollution parameters like Residual Alkali

  20. Identification of Biomarkers for Resistance to Fusarium oxysporum f. sp. cubense Infection and in Silico Studies in Musa paradisiaca Cultivar Puttabale through Proteomic Approach

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    Venkatesh Ramu

    2016-02-01

    Full Text Available Panama wilt caused by Fusarium oxysporum f. sp. cubense (Foc is one of the major disease constraints of banana production. Previously, we reported the disease resistance Musa paradisiaca cv. puttabale clones developed from Ethylmethanesulfonate and Foc culture filtrate against Foc inoculation. Here, the same resistant clones and susceptible clones were used for the study of protein accumulation against Foc inoculation by two-dimensional gel electrophoresis (2-DE, their expression pattern and an in silico approach. The present investigation revealed mass-spectrometry identified 16 proteins that were over accumulated and 5 proteins that were under accumulated as compared to the control. The polyphosphoinositide binding protein ssh2p (PBPssh2p and Indoleacetic acid-induced-like (IAA protein showed significant up-regulation and down-regulation. The docking of the pathogenesis-related protein (PR with the fungal protein endopolygalacturonase (PG exemplify the three ionic interactions and seven hydrophobic residues that tends to good interaction at the active site of PG with free energy of assembly dissociation (1.5 kcal/mol. The protein-ligand docking of the Peptide methionine sulfoxide reductase chloroplastic-like protein (PMSRc with the ligand β-1,3 glucan showed minimum binding energy (−6.48 kcal/mol and docking energy (−8.2 kcal/mol with an interaction of nine amino-acid residues. These explorations accelerate the research in designing the host pathogen interaction studies for the better management of diseases.

  1. Identification of Biomarkers for Resistance to Fusarium oxysporum f. sp. cubense Infection and in Silico Studies in Musa paradisiaca Cultivar Puttabale through Proteomic Approach

    Science.gov (United States)

    Ramu, Venkatesh; Venkatarangaiah, Krishna; Krishnappa, Pradeepa; Shimoga Rajanna, Santosh Kumar; Deeplanaik, Nagaraja; Chandra Pal, Anup; Kini, Kukkundoor Ramachandra

    2016-01-01

    Panama wilt caused by Fusarium oxysporum f. sp. cubense (Foc) is one of the major disease constraints of banana production. Previously, we reported the disease resistance Musa paradisiaca cv. puttabale clones developed from Ethylmethanesulfonate and Foc culture filtrate against Foc inoculation. Here, the same resistant clones and susceptible clones were used for the study of protein accumulation against Foc inoculation by two-dimensional gel electrophoresis (2-DE), their expression pattern and an in silico approach. The present investigation revealed mass-spectrometry identified 16 proteins that were over accumulated and 5 proteins that were under accumulated as compared to the control. The polyphosphoinositide binding protein ssh2p (PBPssh2p) and Indoleacetic acid-induced-like (IAA) protein showed significant up-regulation and down-regulation. The docking of the pathogenesis-related protein (PR) with the fungal protein endopolygalacturonase (PG) exemplify the three ionic interactions and seven hydrophobic residues that tends to good interaction at the active site of PG with free energy of assembly dissociation (1.5 kcal/mol). The protein-ligand docking of the Peptide methionine sulfoxide reductase chloroplastic-like protein (PMSRc) with the ligand β-1,3 glucan showed minimum binding energy (−6.48 kcal/mol) and docking energy (−8.2 kcal/mol) with an interaction of nine amino-acid residues. These explorations accelerate the research in designing the host pathogen interaction studies for the better management of diseases. PMID:28248219

  2. Evaluation of Tea Tree Oil to Control Postharvest Banana Disease of Colletotrichum musae%茶树油控制香蕉采后炭疽病害的研究

    Institute of Scientific and Technical Information of China (English)

    静玮; 张鲁斌; 朱德明; 韩志萍

    2011-01-01

    The potential of using TTO (Tea tree oil) instead of synthetic fungicides to control disease of Colletotrichum musae on banana was evaluated.The antifungal activity of TTO against colony growth of Colletotrichum musae in vitro and its efficiency in vivo on the disease incidence were determined.Results showed that TTO with 20~40 μL could significantly inhibit Colletotrichum musae growth in vitro, and the higher of the dosage of TTO, the higher of antifungal activity.TTO treatment significantly reduced the lesion diameter of inoculated banana.In addition,TTO could remain peel color and fruit firmness at ambient temperature for 10 days.Preliminary study suggested that TTO was feasible on fresh-keeping of postharvest banana fruit.%为了探讨植物提取物对香蕉采后病害的防治效果,以香蕉果实为材料,采用菌丝生长速率方法测定不同剂量的茶树油(TTO)对香蕉炭疽病菌的离体抗菌活性,并用TTO熏蒸处理接种炭疽病菌的香蕉果实,测定果实的发病情况.结果显示,20~40μL TTO能够显著抑制离体炭疽病菌的菌丝生长,浓度越高,抑制效果越明显;接种炭疽病菌的果实经TTO处理后,病斑直径显著小于未处理果实.另外,TTO处理的果实在常温贮藏10 d后果实果皮颜色和硬度也显著优于未处理果实.初步研究表明TTO在控制香蕉果实采后腐烂和保鲜上具有应用可行性.

  3. Correlações entre caracteres da planta e do cacho em bananeira (Musa spp Correlations among characters of the plant and of the bunch in banana (Musa spp.

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    Sérgio Luiz Rodrigues Donato

    2006-02-01

    quantify the relationships among characters development and yield attributes in flowering and harvesting periods of 13 banana (Musa spp. genotypes (varieties and hybrids, in Guanambi State of Bahia, Brazil. The varieties were Prata anã e Pacovan (AAB, Grande naine e Nanicão (AAA, and the hybrids PA42-44, PV42-85, PV42-142, PV42-68 e ST12-31 (AAAB e Ambrosia, Calipso, Bucaneiro e FHIA02 (AAAA, selected in Embrapa Mandioca e Fruticultura. The characters analyzed were plant height; pseudostem perimeter; number of functional leaves in the flowering and in the harvesting; number of days from planting to flowering and to harvesting; number of days from flowering to harvesting; bunch weight, of raquis and of the hands; length and diameter of the peduncle; number of hands and fruits; weight of the second hand; weight, length and diameter of finger and peel thickness. The correlations among the weight of the bunch and the other studied characters, varied among the genotypes and cycles. The associations among the weight of the bunch and the characters of the plant, in a general were no significant, and among the weight of the bunch and the characters of the bunch significant and positive for most of the genotypes in the two cycles evaluated. The correlations among the characters involving all the genotypes along the two cycles was predominantly positive and no significant, however, the associations among the characters of the bunch were in majority significant, positive and with expressive values.

  4. Screenhouse and field persistence of nonpathogenic endophytic Fusarium oxysporum in Musa tissue culture plants.

    Science.gov (United States)

    Paparu, Pamela; Dubois, Thomas; Gold, Clifford S; Niere, Björn; Adipala, Ekwamu; Coyne, Daniel

    2008-04-01

    Two major biotic constraints to highland cooking banana (Musa spp., genome group AAA-EA) production in Uganda are the banana weevil Cosmopolites sordidus and the burrowing nematode Radopholus similis. Endophytic Fusarium oxysporum strains inoculated into tissue culture banana plantlets have shown control of the banana weevil and the nematode. We conducted screenhouse and field experiments to investigate persistence in the roots and rhizome of two endophytic Fusarium oxysporum strains, V2w2 and III4w1, inoculated into tissue-culture banana plantlets of highland cooking banana cultivars Kibuzi and Nabusa. Re-isolation of F. oxysporum showed that endophyte colonization decreased faster from the rhizomes than from the roots of inoculated plants, both in the screenhouse and in the field. Whereas rhizome colonization by F. oxysporum decreased in the screenhouse (4-16 weeks after inoculation), root colonization did not. However, in the field (17-33 weeks after inoculation), a decrease was observed in both rhizome and root colonization. The results show a better persistence in the roots than rhizomes of endophytic F. oxysporum strains V2w2 and III4w1.

  5. Influencia del Tratamiento Osmótico en el Secado de la Banana “Nanica” (Musa cavendishii, L. en Secador de Lecho Fijo Influence of Osmotic Treatment onthe Drying of "Nanica" Bananas (Musa cavendishii, L. in a Fixed Bed Dryer

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    O.C.P. Gaspareto

    2004-01-01

    Full Text Available Se ha estudiado la influencia de la concentración de azúcar (50 y 70 °Brix y de la temperatura (50 y 70 °C en la deshidratación osmótica de la Banana “Nanica” (Musa cavendishii, L.. Para el secado complementario fue empleado un secador de lecho fijo a 60 °C y velocidad del aire 2.0 m/s. Durante el secado se verificó el comportamiento cinético del proceso, usando datos experimentales de los períodos de velocidad decrecientes, obteniéndose curvas de velocidad de secado versus humedad. Basándose en un modelo difusional fueron calculadas las difusividades efectivas, para 50 °Brix/50 °C, 50 °Brix/70 °C, 70 °Brix/50 °C y 70 °Brix/70 °C resultando 9.12x10-10, 9.46x10-10, 1.01x10-09 y 1.05x10-09 m²/s, respectivamente. De los resultados obtenidos, se observó que la difusividad efectiva no estuvo influenciada por la concentración de azúcar y tampoco por la temperatura. Por lo tanto, la mejor condición de pérdida de humedad y ganancia de azúcar fue de 70 °Brix/50 °C.The influence of sugar concentrations (50 and 70 °Brix and temperature (50 and 70 ºC on the osmotic dehydration of "Nanica" Bananas (Musa cavendishii, L., have been studied. For supplementary drying, a fixed bed drier was used at 60 ºC with an air flow of 2.0 m/s. The kinetic behavior of the process was verified during drying using experimental data on the periods of decreasing velocities to obtain curves of drying speed vs humidity. Effective diffusivities were calculated based on a diffusion model for 50 ºBrix/50 ºC, 50 ºBrix/70 ºC, 70 ºBrix/50 ºC and 70 ºBrix/70 ºC, with respective results of 9.12x10-10, 9.46x10-10, 1.01x10-09 and 1.05x10-09 m²/s. It was observed that the effective diffusivities were neither influenced by the sugar concentration nor by the temperature. The best condition of humidity loss and sugar gain was at 70 ºBrix/50º C.

  6. 茶树油熏蒸处理对香蕉采后炭疽病害的影响%Effects of tea tree oil fumigation on banana postharvest disease Colletotrichum musae

    Institute of Scientific and Technical Information of China (English)

    静玮; 苏子鹏; 朱德明; 韩志萍

    2011-01-01

    The effects of natural plants extracts against banana postharvest disease Colletotrichum musae was evaluated.The antifimgal activity of 0~40μL TTO(tea tree oil) and its main components (Terpinenol-4, a-Terpineol, γ-Terpinene and 1,8-Cineole) against colony growth of C.musae in vitro and their effects on disease intensity and defense-related enzymes of banana fruits inoculated with C.musae were determined at 25℃ and 75% relative humidity.Results showed that fumigation of TTO, Terpinenol-4 and a-Terpineol could significantly inhibit C.musae growth in vitro.In addition,the antifungal activity of TTO and its main components could be greatly enhanced with higher levels.Compared with non-treated fruits, 1.25 g/L TTO fumigation reduced lesion diameter of inoculated banana fruits, but less than 0.32 g/L Terpinenol-4 or a-Terpineol.Moreover.1.25 g/L TTO fumigation induced significant increase in peroxidase(POD), and phenylalanine ammonia-lyase(PAL), but decreased polyphenoloxidase(PPO) activity.The preliminary study suggests that TTO fumigation is feasible on fresh-keeping and increasing of disease resistance in postharvest banana fruits,meanwhile, Terpinenol-4 and a-Terpineol are primary antifungal activities of TTO.%为了探讨植物提取物对香蕉采后病害的防治效果,以香蕉果实为材料,采用菌丝生长速率方法测定不同剂量(0~40 μL)的茶树油(TTO)及其主要成分(4-松油烯醇、α-松油醇、γ-松油烯和1,8-桉叶油素)对香蕉炭疽病菌的离体抗菌活性,并用TTO及其主要成分熏蒸处理接种炭疽病菌的香蕉果实,测定果实常温贮藏(25℃,相对湿度75%)的发病情况和TTO处理对接种果实防御酶活性的影响.结果表明,TTO、4-松油烯醇和α-松油醇均能显著抑制离体炭疽病菌的菌丝生长,质量浓度越高,抑制效果越明显:1.25 g/L TTO处理降低了接种炭疽病菌果实的发病程度,0.32 g/L 4-松油烯醇和α-松油醇处理对接种果实病斑直径的

  7. Fructans and other water soluble carbohydrates in vegetative organs and fruits of different Musa spp. accessions.

    Science.gov (United States)

    Cruz-Cárdenas, Carlos I; Miranda-Ham, María L; Castro-Concha, Lizbeth A; Ku-Cauich, José R; Vergauwen, Rudy; Reijnders, Timmy; Van den Ende, Wim; Escobedo-GraciaMedrano, Rosa M

    2015-01-01

    The water soluble carbohydrates (WSC) glucose, fructose, and sucrose are well-known to the great public, but fructans represent another type of WSC that deserves more attention given their prebiotic and immunomodulatory properties in the food context. Although the occurrence of inulin-type fructo-oligosaccharides (FOS) was proposed in the fruit of some banana accessions, little or no information is available neither on the exact identity of the fructan species, nor on the fructan content in different parts of banana plants and among a broader array of banana cultivars. Here, we investigated the WSC composition in leaves, pulp of ripe fruits and rhizomes from mature banana plants of 11 accessions (I to XI), including both cultivated varieties and wild Musa species. High performance anion exchange chromatography with integrated pulsed amperometric detection (HPAEC-IPAD) showed the presence of 1-kestotriose [GF2], inulobiose [F2], inulotriose [F3], 6-kestotriose and 6G-kestotriose (neokestose) fructan species in the pulp of mature fruits of different accessions, but the absence of 1,1-nystose and 1,1,1 kestopentaose and higher degree of polymerization (DP) inulin-type fructans. This fructan fingerprint points at the presence of one or more invertases that are able to use fructose and sucrose as alternative acceptor substrates. Quantification of glucose, fructose, sucrose and 1-kestotriose and principal component analysis (PCA) identified related banana groups, based on their specific WSC profiles. These data provide new insights in the biochemical diversity of wild and cultivated bananas, and shed light on potential roles that fructans may fulfill across species, during plant development and adaptation to changing environments. Furthermore, the promiscuous behavior of banana fruit invertases (sucrose and fructose as acceptor substrates besides water) provides a new avenue to boost future work on structure-function relationships on these enzymes, potentially leading to

  8. Oxidative Stress on Buccal Mucosa Wound in Rats and Rule of Topical Application of Ethanolic Extracts of Mauli Banana ( Musa acuminata Stem

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    Wenda Fitriati Noora

    2015-05-01

    Full Text Available The present study was undertaken to evaluate the effect of a topical application of ethanolic extracts of Mauli banana stem on oxidative status in buccal mucosa wounds of rats. The sets involved three groups, P0 was the negative control; P1 was treated with alocair topically; and P2 was treated with ethanolic extracts of Mauli banana stem topically, respectively, 24h after wound creation for 3 days. The oxidative stress status was evaluated by monitoring the SOD, CAT activity, MDA and CC levels. Ethanolic extracts of Mauli banana stem showed significantly increased in SOD activity, decreased in MDA levels, and no significant change both in CAT activity and CC levels compared to negative control. These results showed that The ethanolic extracts of Mauli banana stem might affect the oxidative stress status during wound healing process.

  9. EFEITOS DE ALGUMAS DROGAS SISTÊMICAS NO CONTROLE PREVENTIVO DO MAL DO PANAMÁ (Fusarim oxysporum f. sp. Cubense (Ef. Sn. & H. DA BANANEIRA (Musa sapientum L. EFFECT OF SOME SYSTEMIC DRUGS IN THE PREVENTIVE CONTROL OF THE MAL-DO-PANAMÁ (Fusarium oxysporum f.sp. cubense (EF. SN. & H. OF THE BANANA TREE (Musa sapientum L.

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    Ildeu Matias do Nascimento

    2007-09-01

    Full Text Available

    Com o objetivo de estudar o efeito de algumas drogas sistêmicas sobre o fungo Fusarium oxysporim f. sp. cubense (Ef. Sn. & H. em tecido de rizoma de bananeira do cultivar Maçã (Musa sapientum L. foi montado um experimento em solo sob cerrado, anteriormente coberto por vegetação natural. Testou-se o efeito do Benlate (benomyl e do Plantvax (oxamyl, ambos nas doses de 0,1, 0,2 e 0,5%, do Mycostatin (nistatina nas doses de 0,02, 0,05 e 0,1% e da combinação Mycostatin + Neantina solúvel (cloreto de metoxi-etil mercúrio 3,7% nas dosagens de 0,02 + 0,15%, 0,05 + 0,3% e 0,1 + 0,5% em banho de imersão dos rizomas Infectados por um período de 10 minutos. Foram usados rizomas com infecção natural pelo patógeno. O delineamento experimental usado foi a casualização completa, com 26 tratamentos e 4 repetições. Cada parcela foi constituída de 4 plantas (covas. Os resultados observados em fevereiro de 1974, revelaram a incidência do ‘Mal do Panamá' indiscriminadamente sobre plantas de todos os tratamentos, demonstrando portanto que nenhum deles foi suficientemente eficaz para erradicar o patógeno estabelecido no tecido do rizoma. Sugeriu-se que a falta de absorção, devida à mutilação de raízes, e a precária penetração dos fungicidas do rizoma, tenham sido responsáveis pelos resultados de insucesso no controle da enfermidade. Concluiu-se que, nas condições em que foram conduzidas essas experiências, e com essa metodologia de aplicação do princípio ativo do fungicida e preparo do rizoma, o controle dessa doença não é possível, nem viável. São sugeridas modificações de procedimento em experiências futuras.

    With the objective of studying the effect of some systemic drugs on the fungus, Fusarium oxysporim f. sp. cubense (Ef. Sn. & H. , which attacks the rootstalk

  10. Dietary intervention with green dwarf banana flour (Musa sp AAA) prevents intestinal inflammation in a trinitrobenzenesulfonic acid model of rat colitis.

    Science.gov (United States)

    Scarminio, Viviane; Fruet, Andrea C; Witaicenis, Aline; Rall, Vera L M; Di Stasi, Luiz C

    2012-03-01

    Dietary products are among the therapeutic approaches used to modify intestinal microflora and to promote protective effects during the intestinal inflammatory process. Because the banana plant is rich in resistant starch, which is used by colonic microbiota for the anaerobic production of the short-chain fatty acids that serve as a major fuel source for colonocytes: first, green dwarf banana flour produces protective effects on the intestinal inflammation acting as a prebiotic and, second, combination of this dietary supplementation with prednisolone presents synergistic effects. For this, we used the trinitrobenzenesulphonic acid (TNBS) model of rat colitis. Our results revealed that the protective effect produced by a combination of 10% green dwarf banana flour with prednisolone was more pronounced than those promoted by a single administration of prednisolone or a diet containing 10% or 20% banana flour. This beneficial effect was associated with an improvement in the colonic oxidative status because the banana flour diet prevented the glutathione depletion and inhibited myeloperoxidase activity and lipid peroxidation. In addition, the intestinal anti-inflammatory activity was associated with an inhibition of alkaline phosphatase activity, a reduction in macroscopic and microscopic scores, and an extension of the lesions. In conclusion, the dietary use of the green dwarf banana flour constitutes an important dietary supplement and complementary medicine product to prevention and treatment of human inflammatory bowel disease.

  11. Trapping Effect of Baxi Banana(Musa AAA Cavendish)Pseudostem on Two Banana Weevil Species%巴西蕉假茎对2种香蕉象甲的诱捕效果分析

    Institute of Scientific and Technical Information of China (English)

    李科明; 许桂莺; 彭正强

    2015-01-01

    This study is to determine the effect of Baxi banana pseudostem as attractant to two banana weevil species , and to provide theoretical guidance for control of banana weevil species. Field traps of pseudostem to banana weevils weredeployed for the analysis,meanwhile,indoor selection response of banana weevil to Baxi banana pseudostem was conducted by using double pitfall olfactometer. Significant trapping effects of Baxi banana pseudostem on two ba⁃nana weevils were found by field trapping and number of the trapped banana weevils in five and ten days reaching 8.3~11.3 and 14.7~18.0 individuals per trap,respectively.Indoor selection response results showed that both the two banana weevils showed significant selection effect to the Baxi banana pseudostem when compared with blank control. Baxi banana pseudostem could be used to control the two banana weevil species.%为明确巴西蕉假茎对香蕉假茎象甲和香蕉球茎象甲的诱捕效果,为利用巴西蕉假茎防治香蕉象甲这一农业防治措施提供理论依据,采用假茎田间诱捕试验及室内选择反应试验,研究了巴西蕉假茎对2种香蕉象甲的诱捕效果。田间诱捕试验结果表明,巴西蕉假茎对2种香蕉象甲具有有效的诱捕作用,其5d和10d的诱捕量分别达8.3~11.3和14.7~18.0头/诱捕器;室内选择反应试验结果表明,与空白对照相比,2种香蕉象甲对巴西蕉假茎均表现出显著的选择趋性。因此,巴西蕉假茎可用于蕉园香蕉象甲的诱捕防治。

  12. Impact of diseases on export and smallholder production of banana.

    Science.gov (United States)

    Ploetz, Randy C; Kema, Gert H J; Ma, Li-Jun

    2015-01-01

    Banana (Musa spp.) is one of the world's most valuable primary agricultural commodities. Exported fruit are key commodities in several producing countries yet make up less than 15% of the total annual output of 145 million metric tons (MMT). Transnational exporters market fruit of the Cavendish cultivars, which are usually produced in large plantations with fixed infrastructures and high inputs of fertilizers, pesticides, and irrigation. In contrast, smallholders grow diverse cultivars, often for domestic markets, with minimal inputs. Diseases are serious constraints for export as well as smallholder production. Although black leaf streak disease (BLSD), which is present throughout Asian, African, and American production areas, is a primary global concern, other diseases with limited distributions, notably tropical race 4 of Fusarium wilt, rival its impact. Here, we summarize recent developments on the most significant of these problems.

  13. Avaliação de cultivares e hibridos de bananeira em lavras, MG Banana hybrids and cultivar evaluation in lavras-MG

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    Lair Victor Pereira

    2003-02-01

    Full Text Available Avaliaram-se os genótipos de bananeira Nam, Pioneira, Caipira, Prata Anã, Grande Naine, SH36-40, FHIA 18, FHIA 01 e PV 03-44, em condições de sequeiro na região sul de Minas Gerais, com o objetivo de identificar os mais produtivos e com melhor adaptação edafoclimática, em comparação com as cultivares Prata e Prata Anã, tradicionalmente cultivadas nessa região. O ensaio foi conduzido na Fazenda Experimental da EPAMIG, em Lavras, no período de 1997 a 1999. Cada genótipo foi plantado em blocos de 50 plantas, no espaçamento 3,0 x 3,0 m, sendo a parcela útil as 24 plantas centrais e uma por repetição. O híbrido SH36-40 foi superior aos demais em peso médio dos cachos (16,56 kg, frutos (171,00 g, diâmetro (4,21 cm e comprimento dos frutos (17,64 cm nos três primeiros ciclos. Isso corresponde a um rendimento de 18,5 t/ha, cerca de duas vezes maior que a produção das cultivares Prata Anã, Pioneira, Nam, Caipira e três vezes maior que a do híbrido PV 03-44. O híbrido SH 36-40 apresentou, ainda, porte médio de 2,75 m, ciclo em torno de 17,5 meses e o menor período da emissão à colheita do cacho, inferior a 4,83 meses para as plantas-mãe, filha e neta. Esse híbrido apresentou também um menor e mais tardio perfilhamento. Os híbridos SH 36-40 e FHIA 01 apresentaram os maiores incrementos nos pesos dos cachos e dos frutos do primeiro para o terceiro ciclo. As cultivares Caipira, Prata Anã, Pioneira e Nam produziram cachos com peso médio inferior a 9,0 kg e o híbrido PV0344, inferior a 6,0 kg. A cv. Caipira foi a mais tardia, com um ciclo em torno de 22 meses e a Prata Anã e Pioneira, as mais precoces, com cerca de 16,6 meses no primeiro ciclo e 15,5 no segundo e terceiro ciclos.The banana genotypes Nam, Caipira, Pioneira, Prata anã, Grande Naine, FHIA 01, FHIA 18, SH 36-44, and PV 03-44 were evaluated from 1997 to 1999. The objective was to identify the most productive and most adaptated genotype compared to the

  14. EFECTO DE LA MICORRIZACIÓN Y LA FERTILIZACIÓN EN LA ACUMULACIÓN DE BIOMASA EN PLANTAS DE BANANO (Musa AAA cv. Gran Enano (Musaceae MICORRHIZATION AND FERTILIZATION EFFECT ON BIOMASS ACCUMULATION IN BANANA PLANTS (Musa AAA cv. Gran Enano (Musaceae

    Directory of Open Access Journals (Sweden)

    Carmen Elena Usuga Osorio

    2008-06-01

    Full Text Available Bajo condiciones de invernadero (ubicado en el municipio de Bello - Antioquia (Colombia se evaluó el efecto independiente y combinado de los factores: tipo de inóculo de Hongos Micorriza Arbuscular (HMA, fertilización y aplicación de materia orgánica sobre el porcentaje de asociación de HMA en plantas de banano (Musa AAA cv. Gran Enano, así como en la acumulación de materia seca foliar y radical. Dentro del factor tipo de inóculo, se evaluaron inóculos nativos, de agroecosistemas bananeros y ecosistemas naturales del Urabá (Antioquia-Colombia, uno comercial y la especie Acaulospora morrowiae; con respecto a la fertilización se probó la mitad, completa y dos veces la dosis de la fertilización recomendada de acuerdo al análisis de suelo y a los requerimientos de la planta, y cada uno de estos factores con y sin la aplicación de materia orgánica; como testigos se usaron, la no aplicación del respectivo factor. Se usó como material vegetal plantas de banano micropropagadas del grupo Cavendish cv. Gran Enano (AAA. El sustrato utilizado para el crecimiento de las plantas de banano se compuso de suelo y arena en relación 70/30 v/v. El suelo se obtuvo de la granja experimental de Augura, ubicado en el municipio de Carepa en la región de Urabá. Los resultados encontrados, muestran que los factores que más incidieron en la asociación así como en la acumulación de biomasa en toda la planta son la micorrización y la adición de materia orgánica. Los resultados, también muestran un comportamiento positivo respecto al uso de inóculos nativos de agroecosistemas bananeros, con bajas aplicaciones de fertilizantes.The effects of independent an combined factors such as inoculum type, fertilization and organic matter application on the percentage of association of ‘H.M.A’ in banana plants (Musa AAA cv. ‘Gran Enano’, and on the accumulation of leaves and rrots material, were evaluated under greenhouse conditions. Natives samples

  15. Effects of hot-water extract of banana (Musa acuminata) fruit's peel on the antibacterial activity, and anti-hypothermal stress, immune responses and disease resistance of the giant freshwater prawn, Macrobrachium rosenbegii.

    Science.gov (United States)

    Rattanavichai, Wutti; Cheng, Winton

    2014-08-01

    The hot-extracts isolated from fruit's peel of banana, Musa acuminata, was evaluated on the antibacterial activity to pathogens from aquatic animals, and immunostimulating potential, disease resistance and anti-hypothermal stress in giant freshwater prawn, Macrobrachium rosenbergii through injection administration. The banana peel extract (BPE) showed good activity against 1 Gram-positive and 3 Gram-negative pathogens, including Lactococcus garvieae, Photobacteria damsella, Vibrio alginolyticus and Vibrio parahemolyticus especially in prawn pathogen of L. garvieae strain, which were carried out by a disk diffusion method. Prawn received BPE via injection administration at 1-6 μg (g prawn)(-1) significantly increased total haemocyte count (THC), hyaline cell (HC), granular cell (GC), phenoloxidase (PO) activity and phagocytic activity against L. garvieae from 3 to 6 days, and significantly increased clearance efficiency against L. garvieae and a significantly decreased coagulation time of prawn from 1 to 6 days. Prawn injected with BPE at 6.0 μg (g prawn)(-1) for 6 days showed significantly increased superoxide dismutase (SOD) activity, but significantly decreased respiratory bursts (RBs) of per haemocyte. Survival rates of M. rosenbergii injected with BPE at concentrations of 1, 3 and 6 μg (g prawn)(-1) were significantly higher than those injected with saline control after challenge with L. garvieae for 4-6 days, and the respective relative survival percentages of prawn were 28.6%, 38.1%, and 47.8%, respectively at 6 days. The sublethal time of prawns that had received saline and BPE at 1, 3 and 6 μg (g prawn)(-1) for 6 days and then were transferred from 28 °C to 14 °C were 69.4, 79.8, 83.6, and 90.2 h, respectively. It was concluded that the BPE can be used as the bacteriostat, and immunostimulant and physiological regulator for prawn through injection administration to enhance immunity, physiological responses, and resistance against L. garvieae.

  16. The effect of banana (Musa acuminata) peels hot-water extract on the immunity and resistance of giant freshwater prawn, Macrobrachium rosenbergii via dietary administration for a long term: Activity and gene transcription.

    Science.gov (United States)

    Rattanavichai, Wutti; Chen, Ying-Nan; Chang, Chin-Chyuan; Cheng, Winton

    2015-10-01

    The non-specific immune parameters, disease resistance and immune genes expressions in Macrobrachium rosenbergii were evaluated at 120 days of post feeding the diets containing the extracts of banana, Musa acuminate, fruit's peel (banana peels extract, BPE) at 0, 1.0, 3.0 and 6.0 g kg(-1). Results showed that prawns fed with a diet containing BPE at the level of 1.0, 3.0 and 6.0 g kg(-1) for 120 days had a significantly higher survival rate (30.0%, 40.0% and 56.7%, respectively) than those fed with the control diet after challenge with Lactococcus garvieae for 144 h, and the respective relative survival percentages were 22.2%, 33.3%, and 51.9%, respectively. Dietary BPE supplementation at 3.0 and/or 6.0 g kg(-1) for 120 days showed a significant increase total haemocyte count (THC), granular cell (GC), superoxide dismutase (SOD) activity, phenoloxidase (PO) activity, transglutaminase (TG) activity, and phagocytic activity and clearance efficiency to L. garvieae infection, and meanwhile, the significant decrease in haemolymph clotting times and respiratory bursts (RBs) per haemocyte of prawns were revealed. Furthermore, the mRNA expressions of prophenoloxidase (proPO), lipopolysaccharide and β-1,3-glucan binding protein (LGBP), peroxinectin (PE), transglutaminase (TG), and crustin (CT) were significantly increased. We therefore recommend that BPE can be used as an immunomodulator for prawns through dietary administration at 6.0 g kg(-1) for a long term (over 120 days) to modify immune responses and genes expression following the enhanced resistance against pathogens.

  17. Fructans and other water soluble carbohydrates in vegetative organs and fruits of different Musa spp. accessions

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    Carlos Ivan eCruz Cardenas

    2015-06-01

    Full Text Available The water-soluble carbohydrates (WSC glucose, fructose and sucrose are well-known to the great public, but fructan represents another type of WSC that deserves more attention given their prebiotic and immunomodulatory properties in the food context. Although the occurrence of inulin-type fructo-oligosaccharides (FOS was proposed in the fruit of some banana accessions, little or no information is available neither on the exact identity of the fructan species, nor on the fructan content in different parts of banana plants and among a broader array of banana cultivars. Here, we investigated the WSC composition in leaves, pulp of ripe fruits and rhizomes from mature banana plants of eleven accessions (I to XI, including both cultivated varieties and wild Musa species. High performance anion exchange chromatography with integrated pulsed amperometric detection (HPAEC-IPAD showed the presence of 1-kestotriose [GF2], inulobiose [F2], inulotriose [F3], 6-kestotriose and 6G-kestotriose (neokestose fructan species in the pulp of mature fruits of different accessions, but the absence of 1,1-nystose and 1,1,1 kestopentaose and higher degree of polymerization (DP inulin-type fructans. This fructan fingerprint points at the presence of one or more invertases that are able to use fructose and sucrose as alternative acceptor substrates. Quantification of glucose, fructose, sucrose and 1-kestotriose and principal component analysis (PCA identified related banana groups, based on their specific WSC profiles. These data provide new insights in the biochemical diversity of wild and cultivated bananas, and shed light on potential roles that fructans may fulfil across species, during plant development and adaptation to changing environments. Furthermore, the promiscuous behavior of banana fruit invertases (sucrose and fructose as acceptor substrates besides water provides a new avenue to boost future work on structure-function relationships on these enzymes

  18. Anthelmintic Activity of Musa paradisiaca (L. cv. Puttabale

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    Venkatesh, V. Krishna

    2013-04-01

    Full Text Available Musa paradisiaca cv. Puttabale (AB group is an indigenous banana cultivar commonly cultivated in the Malnad region of Karnataka, India. Helminthes infections are acute and chronic illness in human beings and cattle. About 3 million people are infected with helminthes worldwide. Traditionally, the plant M. paradisiaca cv. Puttabale was used to expel parasitic worms. In order to justify the ethanomedicinal claim with scientific report, sincere attempts have been made to investigate the Anthelmintic activity from corm ethanol extracts of M. paradisiaca cv. Puttabale using Pheretima posthuma as an experimental model. Three concentrations of 25, 50 and 100 mg/ml of corm ethanol extract were used to study their effect in time of paralysis and death of worm. The results suggest that the ethanol extract at the concentration of 100 mg/ml showed significant effect in time of paralysis at 42.33±1.45 min and death time was 54.00±0.58 min than control group in time of paralysis (142.67±1.45 min and death (168.00±1.53 min. Standard drug piperazine citrate showed paralysis on 39.67±0.88 min and death at 59.00±0.58 min. The corm ethanol extract confirmed antihelmintic activity in dose depend manure and efficient, than standard drug piperazine citrate. This investigation revealed that the antihelmintic property of ethanol extracts of Musa paradisiaca cv. Puttabale against Pheretima posthuma to support its medicinal claims.

  19. Banana Fibers – Variability and Fracture Behaviour

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    Samrat Mukhopadhyay, Ph.D.

    2008-06-01

    Full Text Available Banana fibers obtained from the stem of banana plant (Musa sapientum have been characterised for their diameter variability and their mechanical properties, with a stress on fracture morphology. The nature of representative stress strain curves and fracture at different strain rates have been analysed through SEM.

  20. Fungus-Elicited Metabolites from Plants as an Enriched Source for New Leishmanicidal Agents: Antifungal Phenyl-Phenalenone Phytoalexins from the Banana Plant (Musa acuminata) Target Mitochondria of Leishmania donovani Promastigotes

    Science.gov (United States)

    Luque-Ortega, Juan Román; Martínez, Silvia; Saugar, José María; Izquierdo, Laura R.; Abad, Teresa; Luis, Javier G.; Piñero, José; Valladares, Basilio; Rivas, Luis

    2004-01-01

    Two antifungal phenyl-phenalenone phytoalexins isolated from the banana plant (Musa acuminata) elicited with the fungus Fusarium oxysporum, together with a methoxy derivative of one of them and two epoxide precursors of their chemical synthesis, were tested for leishmanicidal activity on Leishmania donovani promastigotes and L. infantum amastigotes. Drugs inhibited proliferation of both forms of the parasite with a 50% lethal concentration range between 10.3 and 68.7 μg/ml. Their lethal mechanism was found linked to the respiratory chain by a systematic approach, including electron microscopy, measurement of the oxygen consumption rate on digitonin-permeabilized promastigotes, and enzymatic assays on a mitochondrial enriched fraction. Whereas the whole set of compounds inhibited the activity of fumarate reductase in the mitochondrial fraction (50% effective concentration [EC50] between 33.3 and 78.8 μg/ml) and on purified enzyme (EC50 = 53.3 to 115 μg/ml), inhibition for succinate dehydrogenase was only observed for the two phytoalexins with the highest leishmanicidal activity: anigorufone and its natural analogue 2-methoxy-9-phenyl-phenalen-1-one (EC50 = 33.5 and 59.6 μg/ml, respectively). These results provided a new structural motif, phenyl-phenalenone, as a new lead for leishmanicidal activity, and support the use of plant extracts enriched in antifungal phytoalexins, synthesized under fungal challenge, as a more rational and effective strategy to screen for new plant leishmanicidal drugs. PMID:15105102

  1. A Study on the Morphological and PhysicoChemical Characteristics of Five Cooking Bananas

    Science.gov (United States)

    Field evaluation of five banana clones was carried out at the National Germplasm Repository in Miami, Florida, USA from July 2006 to July 2008. Bananas (Musa acuminata Colla [AA, AAA]; Musa x paradisiaca Colla (ABB, AAAB, AABB), are one of the worlds most important food crops. Five clones of cookin...

  2. Ploidy Variation in Hybrids from Interploid 3x X 2x Crosses in Musa

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    Osuji, JO.

    1997-01-01

    Full Text Available Hybrids were obtained after in vitro germination of embryos from interploid crosses between triploid 'French' plantain cultivars (Musa spp. AAB group 'Ntanga 2' and 'Bobby Tannap' with diploid banana (Ivlusa acuminata subsp. burmannicoidesj 'Calcutta 4'. Cross-pollinated bunches were harvested at full maturity and ripened with acetylene in a room for 4 days. Seeds were extracted from peeled ripe fruits by squashing. Embryos from the seeds were excised aseptically after 2 days and germinated in vitro. Seedlings were subsequently planted in early evaluation trials after acclimatising in the greenhouse. Chromosome counts were carried out on root tips of mature and maiden suckers to determine ploidy levels using a modified squashing technique. Counts showed that two of the hybrids were aneuploids (trisomies with somatic chromosome number of 2n = 2x + 1 = 23, one hybrid was diploid while the other two were tetraploids. Tetraploids are the most promising hybrids for the genetic improvement of plantains. Diploids are valuable material for further improvement of the plantain genome at this ploidy level. Trisomies provide means for further characterisation of the Musa genome and physical gene mapping in plantain and banana.

  3. Natural Radioactivity in Bananas

    Science.gov (United States)

    Zagatto, V. A. B.; Medina, N. H.; Okuno, E.; Umisedo, N. K.

    2008-08-01

    The content of 40K natural radionuclide in bananas (Musa sapientum) from the Vale do Ribeira region, São Paulo, Brazil, has been measured. We have collected several samples of bananas prata and nanica, its peels, leaves, and also different soils where the banana tree was planted, such as soil with a standard amount of fertilizer, the fertilizer itself and also soil without fertilizer for comparison. We have used the gamma-ray spectroscopy technique with a NaI(T1) crystal inside a 12 cm thick lead shield to detect the gamma-radiation. The results indicate that only part of the available potassium is absorbed by the plant, which is mainly concentrated in the banana peel.

  4. Characterization of novel microsatellite markers in Musa acuminata subsp. burmannicoides, var. Calcutta 4

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    Amorim Edson P

    2010-05-01

    Full Text Available Abstract Background Banana is a nutritionally important crop across tropical and sub-tropical countries in sub-Saharan Africa, Central and South America and Asia. Although cultivars have evolved from diploid, triploid and tetraploid wild Asian species of Musa acuminata (A genome and Musa balbisiana (B genome, many of today's commercial cultivars are sterile triploids or diploids, with fruit developing via parthenocarpy. As a result of restricted genetic variation, improvement has been limited, resulting in a crop frequently lacking resistance to pests and disease. Considering the importance of molecular tools to facilitate development of disease resistant genotypes, the objectives of this study were to develop polymorphic microsatellite markers from BAC clone sequences for M. acuminata subsp. burmannicoides, var. Calcutta 4. This wild diploid species is used as a donor cultivar in breeding programs as a source of resistance to diverse biotic stresses. Findings Microsatellite sequences were identified from five Calcutta 4 BAC consensi datasets. Specific primers were designed for 41 loci. Isolated di-nucleotide repeat motifs were the most abundant, followed by tri-nucleotides. From 33 tested loci, 20 displayed polymorphism when screened across 21 diploid M. acuminata accessions, contrasting in resistance to Sigatoka diseases. The number of alleles per SSR locus ranged from two to four, with a total of 56. Six repeat classes were identified, with di-nucleotides the most abundant. Expected heterozygosity values for polymorphic markers ranged from 0.31 to 0.75. Conclusions This is the first report identifying polymorphic microsatellite markers from M. acuminata subsp. burmannicoides, var. Calcutta 4 across accessions contrasting in resistance to Sigatoka diseases. These BAC-derived polymorphic microsatellite markers are a useful resource for banana, applicable for genetic map development, germplasm characterization, evolutionary studies and marker

  5. Avaliação de algumas características pós-colheita e índice de aceitação pelos consumidores de novas cultivares de banana Evaluation of some post-harvest characteristics and consumer acceptance index of new banana cultivars

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    Lair Victor Pereira

    2003-06-01

    Full Text Available RESUMO - As cultivares Nam, Pioneira, Caipira, SH 3640, FHIA 01, FHIA 18, Mysore, Nanicão, Grande Naine, Maçã, Prata e Prata Anã foram avaliadas quanto às características de pós-colheita (longevidade pós-colheita, período de comercialização e resistência ao despencamento dos frutos, aspectos visuais (tamanho do fruto e coloração da casca e da polpa, índice de aceitação pelos consumidores por meio da degustação da fruta madura (sabor, aroma, consistência da polpa e semelhança de sabor e aroma com as cultivares tradicionais Prata, Prata Anã, Maçã e Nanicão. O trabalho foi conduzido por meio de ensaios de laboratório usando 6 pencas de bananas de cada cultivar e entrevistas após os testes de degustação com 80 consumidores de Lavras e Maria da Fé-MG, no período de outubro de 2000 a abril de 2001. Pelos resultados, verifica-se que, quanto aos aspectos visuais, somente a cv. Caipira obteve baixo índice de aceitação pelos consumidores (ABSTRACT - The banana cultivars Nam, Pioneira, Caipira, SH 3640, FHIA 01, FHIA 18, Mysore, Nanicão, Grande Naine, Maçã, Prata and Prata Anã were evaluated as to their post-harvest characteristics (post-harvest longevity, marketing period and resistance to fruit dropping, visual aspects (fruit size and coloration of the peel and pulp and consumer acceptance index through tasting of the ripe fruit (flavor, pulp firmness and similarity of flavor with the traditional cultivars Prata, Prata Anã, Maçã and Nanicão. The work was conducted through laboratory trials by using six banana hands of each cultivar and interviews after the tasting tests with 80 consumers of Lavras and Maria da Fé-MG over the period of October 2000 to April 2001.The results pointed out that as to the visual aspects only the cultivar Caipira obtained a low consumer acceptance index (< 50%. As to the post-harvest characteristics the cultivars Prata Anã, Maçã, Prata and SH 3640 stood out with longer post

  6. Molecular Cloning and Expression of MaNPR1E Gene from Banana (Musa acuminata L. AAA group, cv. Cavendish)%香蕉NPR 1E 基因的克隆及表达分析

    Institute of Scientific and Technical Information of China (English)

    王卓; 徐碧玉; 贾彩红; 李健平; 刘菊华; 张建斌; 苗红霞; 金志强

    2015-01-01

    NPR1基因可参与调节植物对病原菌的广谱抗性,在植物系统抗性中起着关键的调控作用。通过RACE (rapid-amplification of cDNA ends)方法从香蕉根系中获得NPR1E基因,命名为MaNPR1E(GenBank登录号分别为: KF582550)。 MaNPR1E是香蕉NPR1基因编码框全长cDNA,包含一个1755 bp的最大开放阅读框,编码一个含584个氨基酸的蛋白质。经蛋白质序列同源比对发现,其含有完整的BTB/POZ结构域、锚蛋白重复ANK序列和NPR1-like-C结构域,属于典型的NPR1蛋白。系统进化树比对分析表明, MaNPR1E与海枣PdNPR3(XP_008808341.1)和油棕EgNPR3(XP_010914123.1)的亲缘关系较近。组织特异性研究表明,该基因组成型表达于香蕉各组织。实时荧光定量PCR分析表明,接种枯萎病菌后MaNPR1E的表达在感病品种中被抑制,而在抗病品种中被激活; MaNPR1E的表达受乙烯利和水杨酸的诱导。上述结果表明, MaNPR1E可能在香蕉抗枯萎病过程中扮演重要的调控角色。%NPR1(nonexpressor of PR gene 1), involved in regulating plant broad-spectrum resistance, plays important roles in plant system resistance. In this study, we report the molecular characteristics of NPR1E gene cloned from banana(Musa acuminate L. AAA group, cv. Cavendish)using a RACE-PCR-based strategy. MaNPR1E(accession number: KF582550) contained an open reading frame of 1 755 bp which encoded a polypeptide of 584 amino acids. Protein alignment showed that they contain the complete typical conserved BTB/POZ (BR-C, ttk and bab/Pox virus and Zinc finger)domain, ankyrin repeats and NPR1/NIM1 like defence protein C terminal, which belongs to a typical NPR1 protein. Phylogenetic analysis showed that the deduced amino acid sequences of MaNPR1E also have high similarity to PdNPR3 (XP_008808341.1) and EgNPR3 (XP_010914123.1) from Phoenix dactylifera and Elaeis guineensis, respectively. Tissue-specific studies showed that the expression of MaNPR1E was constitutive

  7. Amending Storage Vessel and Media Improves Subculture Interval of Musa sp. Tissue Culture Plantlets

    Science.gov (United States)

    Bananas and plantains (Musa sp.) are some of the most important food crops in the world. The USDA-ARS, Tropical Agriculture Research Station Musa spp. collection consists of 140 accessions maintained as clonally propagated plants in field plots as well as in tissue culture. Accessions maintained i...

  8. Dietary supplement of banana (Musa acuminata) peels hot-water extract to enhance the growth, anti-hypothermal stress, immunity and disease resistance of the giant freshwater prawn, Macrobrachium rosenbergii.

    Science.gov (United States)

    Rattanavichai, Wutti; Cheng, Winton

    2015-04-01

    In the present study, Macrobrachium rosenbergii were fed with diets containing extracts of banana, Musa acuminate, fruit's peel (banana peels extract, BPE) at 0, 1.0, 3.0 and 6.0 g kg(-1). The non-specific immune parameters, disease resistance and anti-hypothermal stress were evaluated at 2, 4, 8, 16 and 32 days of post feeding. Also, we demonstrated the percent weight gain (PWG), percent length gain (PLG), feeding efficiency (FE), and survival rate of giant freshwater prawn at 30, 60, 90, and 120 days of post feeding. The PWG, PLG, FE and survival rate of prawns fed at 0, 1.0, 3.0 and 6.0 g kg(-1) BPE-containing diets after 120 days were 69.5%, 75.4%, 77.8% and 83.3%; 21.8%, 23.6%, 27.8% and 33.9%; 0.60, 0.72, 0.75 and 0.90; and 55.4%, 62.2%, 62.3% and 75.3%, respectively. After 32 days of post feeding, a significant increase in total haemocyte count (THC), different haemocyte count (DHC), respiratory bursts (RBs), superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity, phenoloxidase (PO) activity and transglutaminase (TG) activity, and meanwhile, a decreased haemolymph coagulation time was observed. Furthermore, phagocytic activity and clearance efficiency of prawns against Lactococcus garvieae infection were significantly increased. Prawns challenged with L. garvieae after 32 days of feeding at 1.0, 3.0 and 6.0 g kg(-1) had a significantly higher survival rate (33.3%, 40.0% and 56.7%) than those fed with the control diet. Subsequently, hypothermal (14 °C) stress was 43.4%, 50.0% and 50.0%, respectively. Altogether, we therefore recommend the dietary BPE administration at 6.0 g kg(-1) promotes growth, anti-hypothermal stress, and enhance immunity and resistance against L. garvieae in M. rosenbergii.

  9. Hot water treatments delay cold-induced banana peel blackening

    NARCIS (Netherlands)

    Promyou, S.; Ketsa, S.; Doorn, van W.G.

    2008-01-01

    Banana fruit of cv. Gros Michel (Musa acuminata, AAA Group, locally called cv. Hom Thong) and cv. Namwa (Musa x paradisiaca, ABB Group) were immersed for 5, 10 and 15 min in water at 42 degrees C, or in water at 25 degrees C (control), and were then stored at 4 degrees C. Hot water treatment for 15

  10. Effects of inclusion levels of banana (Musa spp.) peelings on feed degradability and rumen environment of cattle fed basal elephant grass.

    Science.gov (United States)

    Nambi-Kasozi, Justine; Sabiiti, Elly Nyambobo; Bareeba, Felix Budara; Sporndly, Eva; Kabi, Fred

    2016-04-01

    The effect of feeding varying banana peeling (BP) levels on rumen environment and feed degradation characteristics was evaluated using three rumen fistulated steers in four treatments. The steers were fed BP at 0, 20, 40, and 60% levels of the daily ration with basal elephant grass (EG) to constitute four diets. Maize bran, cotton seed cake, and Gliricidia sepium were offered to make the diets iso-nitrogenous. The nylon bag technique was used to measure BP and EG dry matter (DM), crude protein (CP), and neutral detergent fiber (NDF) degradabilities at 0, 6, 12, 24, 48, 72, 96, and 120 h. Rumen fluid samples were collected to determine pH and volatile fatty acid (VFA) concentrations. Effective DM, CP, and NDF degradabilities of BP ranged between 574 and 807, 629-802, and 527-689 g/kg, respectively, being lower at higher BP levels. Elephant grass degradability behaved similarly with relatively high effective CP degradability (548-569 g/kg) but low effective DM and NDF degradability (381-403 and 336-373 g/kg, respectively). Rumen pH and VFA reduced with increasing BP in the diets. Rumen pH dropped to 5.8 and 5.9 at the 40 and 60% BP feeding levels, respectively. Banana peelings were better degraded than EG but higher BP levels negatively affected feed degradability and rumen environment.

  11. Avaliação de cultivares e híbridos de bananeira no recôncavo baiano Evaluation of banana cultivars and hybrids in northeastern Bahia

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    Marcelo Bezerra Lima

    2005-06-01

    Full Text Available O programa de melhoramento genético de bananeira da Embrapa Mandioca e Fruticultura Tropical visa desenvolver e selecionar novas cultivares de bananeira com alta produtividade, bom sabor dos frutos e que apresentem resistência às principais doenças. Objetivou-se com este trabalho avaliar cultivares e híbridos de bananeira visando a identificação de genótipos superiores em produtividade e resistência a doenças. Foram avaliados os híbridos da cv. Gros Michel (Calipso, Bucaneiro e Ambrosia; da cv.Yangambi (YB42-21 e YB42-17; da cv. Prata (ST42-08 e ST12-31 e de Prata-Anã (SH3640 e as cultivares Pacovan e Nanicão. As características utilizadas para as avaliações foram: altura da planta (m; diâmetro do pseudocaule (cm; número de folhas vivas na floração; número de folhas vivas na colheita; número de frutos por cacho; peso do cacho (kg; peso médio de frutos (g; comprimento do fruto (cm; diâmetro do fruto (cm; espessura da casca (mm; número de dias do plantio à floração e número de dias do plantio à colheita. Usou-se o delineamento em blocos casualizados com 10 tratamentos, cinco repetições e seis touceiras por parcela, espaçadas de 2,00 m x 3,00 m. Os híbridos avaliados apresentaram características agronômicas iguais ou superiores as cultivares que lhes deram origem. Os melhores híbridos de 'Prata', 'Yangambi' e 'Gros Michel' foram respectivamente, ST12-31, YB42-21 e Bucaneiro.The banana genetic breeding program at Embrapa Cassava and Tropical Fruits aims to develop and select new banana varieties with high productivity, good fruit flavor and resistance to most banana diseases. The objective of this work was to evaluate banana cultivars and hybrids in order to identify superior genotypes for productivity and disease resistance. The following characteristics were evaluated: plant height (cm; pseudostem diameter (cm; number of living leaves during flowering; and at harvest number of fingers per bunch; bunch weight

  12. Effects of high CO2 treatment on green-ripening and peel senescence in banana and plantain fruits

    Institute of Scientific and Technical Information of China (English)

    SONG Mu-bo; TANG Lu-ping; ZHANG Xue-lian; BAI Mei; PANG Xue-qun; ZHANG Zhao-qi

    2015-01-01

    Banana fruit (Musa, AAA group, cv. Brazil) peel fails to ful y degreen but the pulp ripens normal y at temperatures above 24°C. This abnormal ripening, known as green-ripening, does not occur in plantains (Musa, ABB group, cv. Dajiao). Based on the fact that un-completely yel owing was also observed for bananas in poorly ventilated atmospheres, in the present study, the effect of high CO2 with regular O2 (21%) on banana ripening was investigated along with that on plantains at 20°C. The results showed that high CO2 conferred different effects on the color changing of bananas and plantains. After 6 d ripening in 20%CO2, plantains ful y yel owed, while bananas retained high chlorophyl content and stayed green. In contrast to the differentiated color changing patterns, the patterns of the softening, starch degradation and soluble sugar accumulation in the pulp of 20%CO2 treated bananas and plantains displayed similarly as the patterns in the fruits ripening in regular air, indicating that the pulp ripening was not inhibited by 20%CO2, and the abnormal ripening of bananas in 20%CO2 can be considered as green ripening. Similar expression levels of chlorophyl degradation related genes, SGR, NYC and PaO, were detected in the peel of the control and treated fruits, indicating that the repressed degreening in 20%CO2 treated bananas was not due to the down-regulation of the chlorophyl degradation related genes. Compared to the effect on plantains, 20%CO2 WUHDWmHQW GHOD\\HG WKH GHFOLQH LQ WKH FKORURSK\\O ÀRUHVFHQFH Fv/Fm) values and in the mRNA levels of a gene coding smal subunit of Rubisco (SSU), and postponed the disruption of the ultrastructure of chloroplast in the peel tissue of bananas, indicating that the senescence of the green cel s in the exocarp layer was delayed by 20%CO2, to more extent in bananas than in plantains. High CO2 reduced the ethylene production and the expression of the related biosynthesis gene, ACS, but elevated the respiration rates in both

  13. Intellectual property protection through the geographical indication “Chã de Jardim” of the banana leaf handicrafts (musa sp. in the State of Paraiba

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    Everaldo de França

    2015-11-01

    Full Text Available The construction of a Brazilian Geographical Indication System has been guided by a far wider range of goods, ranging from industrial and agricultural products, services, and even craftwork. Taking into account social, cultural and geographical characteristics, as well as the ethnic constitution of the population of Areia city in the State of Paraiba (Brazil, it is possible to suppose the vast local knowledge that this city’s communities have accumulated over the decades while they seek to incorporate such knowledge in their daily practices. One of the artesanal activities subject to protection of Geographical Indication (IG is the use of banana’s plant (Musa sp. stem as a raw material for some  manufactured products such as handbags, light fixtures, folders for events, cooking utensils etc. The IG records for this type of activity can value the local knowledge regarding the sustainable use of biodiversity and promote the cultural traditions of Areia city, bringing also improvements to the communities involved and consequently increasing the regional development. The identified geographical name to be protected for craftwork is “Chã de Jardim”. It is suggested that “Chã de Jardim” becomes protected by indication of origin whereas the artesanal products of this region feature good reputation. JEL-Code | O33; Q56; R11.

  14. Controle pós-colheita da antracnose da banana -prata anã tratada com fungicidas e mantida sob refrigeração Dwarf silver banana post-harvest anthracnose control with fungicides and under refrigeration

    Directory of Open Access Journals (Sweden)

    Ana Flávia Santos Coelho

    2010-08-01

    Full Text Available A antracnose é uma das principais doenças após a colheita da banana, causada pelas diferentes raças fisiológicas do fungo Colletotrichum musae, e que se manifesta na maioria das vezes na fruta madura, comprometendo a sua qualidade. Seu aparecimento está relacionado ao manuseio inadequado, ausência de controle químico em campo e de refrigeração. A aplicação de produtos químicos é efetuada durante o beneficiamento, sendo importante salientar que pode iniciar com a fruta ainda no campo. Objetivou-se, neste trabalho, verificar o efeito de fungicidas utilizados após a colheita, visando o controle da antracnose em bananas, cultivar Prata Anã, armazenadas a 20ºC e 12ºC. Para isso, foi testada a ação dos fungicidas tiabendazol e imazalil no controle de Colletotrichum musae. No teste, foram empregados isolados das cultivares Prata Anã, FHIA 02 e ST 4208, inoculados em bananas-prata anã. As avaliações foram efetuadas a cada 3 dias, por meio de medições do tamanho das lesões (mm², sendo as frutas inoculadas descartadas no estádio de maturação 7 (amarelo com pontas marrom. Os tratamentos mais eficientes no controle da doença foram aqueles em que as bananas foram tratadas com o fungicida tiabendazol. No estudo, também foi verificada diferença em relação à patogenicidade dos isolados utilizados, que demonstraram especificidade quanto a cultivar.Anthracnose is one of the main post-harvest disease of the banana, caused by different physiological races of Colletotrichum musae. It manifests itself mostly in ripe fruits, threatening its quality. Its appearance is related to the inadequate handling, absence of chemical control in field, and cooling. Application of chemical products is usually done during the processing, but it is important to point out that it can be done while the fruit is still in the field. This work had as an objective to verify the effect post-harvest application of fungicides to control anthracnose in

  15. Assessment of In Vivo Antidiabetic Properties of Umbelliferone and Lupeol Constituents of Banana (Musa sp. var. Nanjangud Rasa Bale) Flower in Hyperglycaemic Rodent Model.

    Science.gov (United States)

    Ramu, Ramith; S Shirahatti, Prithvi; S, Nanjunda Swamy; Zameer, Farhan; Lakkappa Dhananjaya, Bhadrapura; M N, Nagendra Prasad

    2016-01-01

    Banana is an extensively cultivated plant worldwide, mainly for its fruit, while its ancillary product, the banana flower is consumed as a vegetable and is highly recommended for diabetics in the traditional Indian medicine system. This study is based on an investigation of the in vivo antihyperglycaemic activity of Umbelliferone (C1) and Lupeol (C2) isolated from the ethanol extract of banana flower (EF) in alloxan induced diabetic rat model. Diabetic rats which were administered with C1, C2 and EF (100 and 200 mg/kg b. wt.) for 4 weeks showed deterioration in fasting hyperglycaemia and reversal of abnormalities in serum/urine protein, urea and creatinine, when compared to the diabetic control group of rats. The diabetic group of rats fed with EF, C1 and C2 (100 mg/kg b. wt.) once daily, for a period of 28 days resulted in a significant reduction of diabetic symptoms viz., polyphagia, polydipsia, polyuria and urine sugar together with an improved body weight. HbA1c extent was reduced whereas levels of insulin and Hb were increased. Both the extract and compounds wielded positive impacts in diabetic rats by reversal of altered activities of hepatic marker enzymes viz., aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP); glycolytic enzyme (hexokinase); shunt enzyme (glucose-6-phosphate dehydrogenase); gluconeogenic enzymes (glucose-6-phosphatase, fructose-1,6-bisphosphatase, lactate dehydrogenase) and pyruvate kinase. The characteristic diabetic complications such as hypercholesterolemia and hypertriacylglycerolemia also significantly reverted to normal in the serum/liver of diabetic rats. Besides these, the treatment increased the activities of enzymatic and non-enzymatic antioxidants in the serum and liver. The histological observations revealed a marked regeneration of the β-cells in the drug treated diabetic rats. In conclusion, the present study illustrates that EF, C1 and C2 enhances the glycolytic activities, besides

  16. Assessment of In Vivo Antidiabetic Properties of Umbelliferone and Lupeol Constituents of Banana (Musa sp. var. Nanjangud Rasa Bale Flower in Hyperglycaemic Rodent Model.

    Directory of Open Access Journals (Sweden)

    Ramith Ramu

    Full Text Available Banana is an extensively cultivated plant worldwide, mainly for its fruit, while its ancillary product, the banana flower is consumed as a vegetable and is highly recommended for diabetics in the traditional Indian medicine system. This study is based on an investigation of the in vivo antihyperglycaemic activity of Umbelliferone (C1 and Lupeol (C2 isolated from the ethanol extract of banana flower (EF in alloxan induced diabetic rat model. Diabetic rats which were administered with C1, C2 and EF (100 and 200 mg/kg b. wt. for 4 weeks showed deterioration in fasting hyperglycaemia and reversal of abnormalities in serum/urine protein, urea and creatinine, when compared to the diabetic control group of rats. The diabetic group of rats fed with EF, C1 and C2 (100 mg/kg b. wt. once daily, for a period of 28 days resulted in a significant reduction of diabetic symptoms viz., polyphagia, polydipsia, polyuria and urine sugar together with an improved body weight. HbA1c extent was reduced whereas levels of insulin and Hb were increased. Both the extract and compounds wielded positive impacts in diabetic rats by reversal of altered activities of hepatic marker enzymes viz., aspartate transaminase (AST, alanine transaminase (ALT, alkaline phosphatase (ALP; glycolytic enzyme (hexokinase; shunt enzyme (glucose-6-phosphate dehydrogenase; gluconeogenic enzymes (glucose-6-phosphatase, fructose-1,6-bisphosphatase, lactate dehydrogenase and pyruvate kinase. The characteristic diabetic complications such as hypercholesterolemia and hypertriacylglycerolemia also significantly reverted to normal in the serum/liver of diabetic rats. Besides these, the treatment increased the activities of enzymatic and non-enzymatic antioxidants in the serum and liver. The histological observations revealed a marked regeneration of the β-cells in the drug treated diabetic rats. In conclusion, the present study illustrates that EF, C1 and C2 enhances the glycolytic activities

  17. MULTIPLICACIÓN DE HONGOS MICORRIZA ARBUSCULAR (H.M.A Y EFECTO DE LA MICORRIZACIÓN EN PLANTAS MICROPROPAGADAS DE BANANO (Musa AAA cv. Gran Enano (Musaceae MULTIPLICATION OF ARBUSCULAR MYCORRHIZAE FUNGI (AMF AND MYCORRHIZATION EFFECT IN MICROPROPAGATED PLANTS OF BANANA (Musa AAA cv. ‘Gran Enano’ (Musaceae

    Directory of Open Access Journals (Sweden)

    Carmen Elena Usuga Osorio

    2008-06-01

    Full Text Available Se evaluó el proceso de multiplicación de hongos que forman micorriza arbuscular (HMA, para lo cual se usaron diferentes tipos de inóculos entre ellos nativos de agroecosistemas bananeros del Urabá (Antioquia-Colombia, en sustrato sólido, con diferentes plantas hospedadoras y la infectividad y efectividad sobre plantas de banano (Musa AAA cv. Gran Enano. La colonización micorrizal promedio general de los HMA a las plantas trampa fue de 37,76 ± 21,86 %, con respecto a este porcentaje, las plantas B (Brachiaria decumbens y S (Sorgum vulgare fueron las que más favorecieron la simbiosis. Teniendo en cuenta el sustrato, el S2 (Arena 50 - suelo 50 y el S6 (Vermiculita 50-suelo 50 permitieron expresiones significativamente mayores respecto a los demás. El Sorgum vulgare y Pueraria phaseoloides y en el sustrato S1 (Arena 30 - suelo 70, se encontró un mayor número de esporas. La combinación planta-sustrato que más favoreció la asociación fue la planta trampa B en los sustratos S2 y S4 (cascarilla de arroz 50-suelo50 y la producción de esporas fueron las plantas K y S en el sustrato S1. La asociación micorrícica general en plantas de banano provenientes de cultivo de tejidos fue de 48,74 ± 30,44. No se encontraron diferencias significativas (P > 0,05 entre plantas de cero días con plantas de 30 de aclimatadas. Los inóculos que significativamente favorecieron la asociación fueron los provenientes de agroecosistemas bananeros al compararse con el inóculo comercial y el proveniente de ecosistemas naturales del Urabá. El mayor peso seco foliar y radical se encontró en plántulas de banano inoculadas con I5 (Inóculo proveniente de agroecosistema bananeros de la zona de estudio. Para las variables de crecimiento no se encontraron diferencias.The process of multiplication of arbuscular mycorrhizae fungi (AMF from indigenous banana agro-environments from Urabá (Antioquia - Colombia was evaluated, using solid substrate, with different

  18. Dynamics of Growth and Development of Banana (Musa AAA Simmonds cvs. Gran Enano and Valery Dinámica del Crecimiento y Desarrollo del Banano (Musa AAA Simmonds cvs. Gran Enano y Valery

    Directory of Open Access Journals (Sweden)

    Ana María Martínez Acosta

    2011-12-01

    Full Text Available This research was conducted in the banana zone on Urabá (Colombia, whith cvs. Gran Enano and Valery. Since planting, each three to four leaves sprouted, three plants per variety were sampled, each corresponding to a repetition. These were separated into its different organs and the total dry matter was estimated. Each cv. was analyzed on a growth curve. The dry matter accumulation on both cvs. is adjusted to the typical sigmoid curve of the plant growth. In the exponential phase, the corm was the main source of assimilates for the development; while in the lineal and senescence phase, the pseudo-stem and leaves were the reservoir organs; when the bunch is formed, such reserves were sent to this drain. In general, while the development progressed, the vegetative organs did not show any dry matter lost, only reduction in the assimilates accumulation rate; unlike the bunch that from its emission kept a high rate, leading the fruit to represent, in the harvest season, almost 50% of the total dry matter of the plant.El estudio se realizó en la zona bananera de Urabá (Colombia; con los cvs. Gran Enano y Valery. A partir de la siembra, cada tres a cuatro hojas emitidas, se muestrearon tres plantas por cv., cada una correspondiente a una repetición. Estas se separaron en sus diferentes órganos y se estimó la materia seca total. Se analizó cada cv. a partir de curvas de crecimiento. La acumulación de materia seca en ambos cvs. se ajustó al modelo típico de la curva sigmoidea del crecimiento vegetal. En la fase exponencial, el cormo fue la principal fuente de asimilados para el desarrollo;mientras que en la fase lineal y de senescencia,el pseudotallo y hojas fueron órganos reservorio; al formarse el racimo, tales reservas fueron enviadas a ese sumidero. En general, a medida que avanzaba el desarrollo, los órganos vegetativos no presentaron pérdidas de materia seca, solo disminución en la tasa de acumulación de asimilados;a diferencia del

  19. Characterization of differential ripening pattern in association with ethylene biosynthesis in the fruits of five naturally occurring banana cultivars and detection of a GCC-box-specific DNA-binding protein.

    Science.gov (United States)

    Choudhury, Swarup Roy; Roy, Sujit; Saha, Progya Paramita; Singh, Sanjay Kumar; Sengupta, Dibyendu N

    2008-07-01

    MA-ACS1 and MA-ACO1 are the two major ripening genes in banana and play crucial role in the regulation of ethylene production during ripening. Here, we report a comparative ripening pattern in five different naturally occurring banana cultivars namely Cavendish (AAA), Rasthali (AAB), Kanthali (AB), Poovan (AAB) and Monthan (ABB), which have distinct genome composition. We found a distinct variation in the climacteric ethylene production and in-vivo ACC oxidase activity level during the ripening stages in the five cultivars. We identified the cDNAs for MA-ACS1 and MA-ACO1 from the five cultivars and studied the transcript accumulation patterns of the two genes, which correlated well with the differential timing in the expression of these two genes during ripening. The GCC-box is one of the ethylene-responsive elements (EREs) found in the promoters of many ethylene-inducible genes. We have identified a GCC-box motif (putative ERE) in the promoters of MA-ACS1 and MA-ACO1 in banana cultivars. DNA-protein interaction studies revealed the presence of a GCC-box-specific DNA-binding activity in the fruit nuclear extract and such DNA-binding activity was enhanced following ethylene treatment. South-Western blotting revealed a 25-kDa nuclear protein that binds specifically to GCC-box DNA in the climacteric banana fruit. Together, these results indicate the probable involvement of the GCC-box motif as the cis-acting ERE in the regulation of MA-ACS1 and MA-ACO1 during ripening in banana fruits via binding of specific ERE-binding protein.

  20. Effects of relative humidity on banana fruit drop

    NARCIS (Netherlands)

    Saengpook, C.; Ketsa, S.; Doorn, van W.G.

    2007-01-01

    Commercial ripening of banana fruit occurs at high relative humidity (RH), which prevents browning of damaged skin areas. In experiments with ripening at high RH (94 ± 1%) the individual fruit (fingers) of `Sucrier¿ (Musa acuminata, AA Group) banana exhibited a high rate of drop. The falling off of

  1. Modelling runoff at the plot scale taking into account rainfall partitioning by vegetation: application to stemflow of banana (Musa spp. plant

    Directory of Open Access Journals (Sweden)

    Y.-M. Cabidoche

    2009-06-01

    Full Text Available Rainfall partitioning by vegetation modifies the intensity of rainwater reaching the ground, which affects runoff generation. Incident rainfall is intercepted by the plant canopy and then redistributed into throughfall and stemflow. Rainfall intensities at the soil surface are therefore not spatially uniform, generating local variations of runoff production that are disregarded in runoff models. The aim of this paper was to model runoff at the plot scale, accounting for rainfall partitioning by vegetation in the case of plants concentrating rainwater at the plant foot and promoting stemflow. We developed a lumped modelling approach, including a stemflow function that divided the plot into two compartments: one compartment including stemflow and the relative water pathways and one compartment for the rest of the plot. This stemflow function was coupled with a production function and a transfer function to simulate a flood hydrograph using the MHYDAS model. Calibrated parameters were a "stemflow coefficient", which compartmented the plot; the saturated hydraulic conductivity (Ks, which controls infiltration and runoff; and the two parameters of the diffusive wave equation. We tested our model on a banana plot of 3000 m2 on permeable Andosol (mean Ks=75 mm h−1 under tropical rainfalls, in Guadeloupe (FWI. Runoff simulations without and with the stemflow function were performed and compared to 18 flood events from 10 to 130 mm rainfall depth. Modelling results showed that the stemflow function improved the calibration of hydrographs according to the error criteria on volume and on peakflow and to the Nash and Sutcliffe coefficient. This was particularly the case for low flows observed during residual rainfall, for which the stemflow function allowed runoff to be simulated for rainfall intensities lower than the Ks measured at the soil surface. This approach also allowed us to take into account the experimental data, without needing to calibrate

  2. 香蕉ARF3基因全长克隆及其对枯萎病菌的响应特性分析%Cloning the Full-length cDNA of ARF3 Gene from Banana (Musa acuminata) and Characterizing Its Response to Banana Fusarium

    Institute of Scientific and Technical Information of China (English)

    杨浩; 杜中军; 徐立; 唐志鹏; 李志英

    2013-01-01

    ARF3 gene is one of member of the ARF gene family. The functions of ARF3 gene are considered to involve in cell signal transduction, membrane transportation and vesicle transporting. This study has designed four specific primers according to the core sequence fragment, which was obtained from banana transcriptome. The full cDNA of the ARF3 was cloned from banana (Musa acuminata) by using the approaches of RACE and PCR, the cloned gene was named MuARF3. The gene sequence of 1 427 bp contains an open reading frame (ORF) of 1 166 bp in length that encodes 338 amino acid residuals. BLAST analysis showed that the full-length nucleotide sequence of the ARF3 has a range from 69% to 74% similarities to the reported ARF3 gene of the other plants as well as from 81% to 93% similarities in amino acid sequence. Through infecting banana roots inoculated with banana Fusarium race 4, the expression level of MuARF3 was dramatically elevated to the highest point at 12 h after inoculation and then gradually declined with time elapse. Therefore, the cloned MuARF3 gene has active response to infection of Fusarium physiological race No.4, which might provide a reference for the further use of the gene.%  ARF3基因属于ARF基因家族的成员,主要功能是参与细胞信号转导、跨膜运输及囊泡转运.本研究根据已获得的基因片段设计4条特异性引物,采用RACE技术克隆获得了香蕉ARF3基因cDNA全长序列,命名为MuARF3.该基因序列全长共1427 bp,开放阅读框长度为1166 bp,共编码338个氨基酸.BLAST结果显示,香蕉ARF3基因全长核苷酸序列与已报道的其它植物ARF3具有69%~74%的相似性,氨基酸序列有81%~93%的相似性.用香蕉枯萎病菌4号生理小种侵染香蕉根系后,分析得出的结果表明,侵染根系后香蕉ARF3基因的表达量在侵染后12 h达到最高,并且随着时间推移逐渐降低.由此说明本研究获得的MuARF3基因对香蕉枯萎病菌4号生理小种侵染具有应急

  3. Dynamic headspace solid-phase microextraction combined with one-dimensional gas chromatography-mass spectrometry as a powerful tool to differentiate banana cultivars based on their volatile metabolite profile.

    Science.gov (United States)

    Pontes, Marisela; Pereira, Jorge; Câmara, José S

    2012-10-15

    In this study the effect of the cultivar on the volatile profile of five different banana varieties was evaluated and determined by dynamic headspace solid-phase microextraction (dHS-SPME) combined with one-dimensional gas chromatography-mass spectrometry (1D-GC-qMS). This approach allowed the definition of a volatile metabolite profile to each banana variety and can be used as pertinent criteria of differentiation. The investigated banana varieties (Dwarf Cavendish, Prata, Maçã, Ouro and Platano) have certified botanical origin and belong to the Musaceae family, the most common genomic group cultivated in Madeira Island (Portugal). The influence of dHS-SPME experimental factors, namely, fibre coating, extraction time and extraction temperature, on the equilibrium headspace analysis was investigated and optimised using univariate optimisation design. A total of 68 volatile organic metabolites (VOMs) were tentatively identified and used to profile the volatile composition in different banana cultivars, thus emphasising the sensitivity and applicability of SPME for establishment of the volatile metabolomic pattern of plant secondary metabolites. Ethyl esters were found to comprise the largest chemical class accounting 80.9%, 86.5%, 51.2%, 90.1% and 6.1% of total peak area for Dwarf Cavendish, Prata, Ouro, Maçã and Platano volatile fraction, respectively. Gas chromatographic peak areas were submitted to multivariate statistical analysis (principal component and stepwise linear discriminant analysis) in order to visualise clusters within samples and to detect the volatile metabolites able to differentiate banana cultivars. The application of the multivariate analysis on the VOMs data set resulted in predictive abilities of 90% as evaluated by the cross-validation procedure.

  4. Modelling runoff at the plot scale taking into account rainfall partitioning by vegetation: application to stemflow of banana (Musa spp. plant

    Directory of Open Access Journals (Sweden)

    Y.-M. Cabidoche

    2009-11-01

    Full Text Available Rainfall partitioning by vegetation modifies the intensity of rainwater reaching the ground, which affects runoff generation. Incident rainfall is intercepted by the plant canopy and then redistributed into throughfall and stemflow. Rainfall intensities at the soil surface are therefore not spatially uniform, generating local variations of runoff production that are disregarded in runoff models. The aim of this paper was to model runoff at the plot scale, accounting for rainfall partitioning by vegetation in the case of plants concentrating rainwater at the plant foot and promoting stemflow. We developed a lumped modelling approach, including a stemflow function that divided the plot into two compartments: one compartment including stemflow and the related water pathways and one compartment for the rest of the plot. This stemflow function was coupled with a production function and a transfer function to simulate a flood hydrograph using the MHYDAS model. Calibrated parameters were a "stemflow coefficient", which compartmented the plot; the saturated hydraulic conductivity (Ks, which controls infiltration and runoff; and the two parameters of the diffusive wave equation. We tested our model on a banana plot of 3000 m2 on permeable Andosol (mean Ks=75 mm h−1 under tropical rainfalls, in Guadeloupe (FWI. Runoff simulations without and with the stemflow function were performed and compared to 18 flood events from 10 to 140 rainfall mm depth. Modelling results showed that the stemflow function improved the calibration of hydrographs according to the error criteria on volume and on peakflow, to the Nash and Sutcliffe coefficient, and to the root mean square error. This was particularly the case for low flows observed during residual rainfall, for which the stemflow function allowed runoff to be simulated for rainfall intensities lower than the Ks measured at the soil surface. This approach also allowed us to take into account the experimental data

  5. Modelling runoff at the plot scale taking into account rainfall partitioning by vegetation: application to stemflow of banana (Musa spp.) plant

    Science.gov (United States)

    Charlier, J.-B.; Moussa, R.; Cattan, P.; Cabidoche, Y.-M.; Voltz, M.

    2009-11-01

    Rainfall partitioning by vegetation modifies the intensity of rainwater reaching the ground, which affects runoff generation. Incident rainfall is intercepted by the plant canopy and then redistributed into throughfall and stemflow. Rainfall intensities at the soil surface are therefore not spatially uniform, generating local variations of runoff production that are disregarded in runoff models. The aim of this paper was to model runoff at the plot scale, accounting for rainfall partitioning by vegetation in the case of plants concentrating rainwater at the plant foot and promoting stemflow. We developed a lumped modelling approach, including a stemflow function that divided the plot into two compartments: one compartment including stemflow and the related water pathways and one compartment for the rest of the plot. This stemflow function was coupled with a production function and a transfer function to simulate a flood hydrograph using the MHYDAS model. Calibrated parameters were a "stemflow coefficient", which compartmented the plot; the saturated hydraulic conductivity (Ks), which controls infiltration and runoff; and the two parameters of the diffusive wave equation. We tested our model on a banana plot of 3000 m2 on permeable Andosol (mean Ks=75 mm h-1) under tropical rainfalls, in Guadeloupe (FWI). Runoff simulations without and with the stemflow function were performed and compared to 18 flood events from 10 to 140 rainfall mm depth. Modelling results showed that the stemflow function improved the calibration of hydrographs according to the error criteria on volume and on peakflow, to the Nash and Sutcliffe coefficient, and to the root mean square error. This was particularly the case for low flows observed during residual rainfall, for which the stemflow function allowed runoff to be simulated for rainfall intensities lower than the Ks measured at the soil surface. This approach also allowed us to take into account the experimental data, without needing to

  6. In vitro mutants identification of banana (Musa sp.) with tolerance to toxin from Fusarium oxysporum f. sp cubense, treating buds with several gamma radiation doses; Identificacao in vitro de mutantes de banana maca (Musa sp.) tolerantes a toxina do Fusarium oxysporum f. sp. cubense, a partir de gemas tratadas com diferentes doses de radiacao gama

    Energy Technology Data Exchange (ETDEWEB)

    Moura, Adriana Muniz Mendes de; Houllou-Kido, Laureen Michelle; Franca, Jose Geraldo Eugenio de [Empresa Pernambucana de Pesquisa Agropecuaria, Recife, PE (Brazil); Colaco, Waldeciro [Pernambuco Univ., Recife, PE (Brazil). Dept. de Energia Nuclear

    1999-11-01

    Mutants of banana, obtained through treatment with different level of gamma-radiation (0; 10; 20; 30; 40 Gy), were initially cultivated in vitro in medium for rapid clonal propagation during 30 days. These treatment affected the shoot tips development ratio. Some plants developed necrosis and died, but some of the shoot tips emitted new gems. These material were cultivated in medium 20% of the toxin of Fusarium oxysporum cubense. During the selection period, the necrosis occurrence and death of susceptible shoot tips were observed. Whereas the tolerant shoot tips kept themselves green during the entire selection process. At the end of the selection process, eight shoot tips were obtained. (author) 7 refs.

  7. CORRELATIONS BETWEEN COMMERCIALIZATION UNITS OF BANANA "NANICÃO" (Musa acuminata AAA IN GOIÁS, BRAZIL CORRELAÇÕES ENTRE UNIDADES DE COMERCIALIZAÇÃO PARA BANANAS “NANICÃO” (Musa acuminata AAA EM GOIÁS

    Directory of Open Access Journals (Sweden)

    Ronaldo Veloso Naves

    2007-09-01

    Full Text Available

    In Goiás, wholesalers of the “Giant Cavendish” banana use different systems of measuring. This causes constant confusion. This study was undertaken to try to establish empirical formulas which can be used to correlate the diverse systems.

    Na comercialização de bananas do sub-grupo cavendishi, em Goiás, utiliza-se de várias unidades de medida. Esta diversidade causa problemas no bom entendimento entre vendedores e compradores. Com a finalidade de estabelecer fórmulas empíricas de transformações de unidades de comercialização, empreendeu-se o presente trabalho. Com base nos resultados, conclui-se que as melhores fórmulas de transformações são as seguintes: a - Peso de um carregamento em cachos para número de frutas (NF = 80,4 P/ 11,41, sendo P tomado em Kg; b - Para a obtenção do peso líquido ( PL de frutas a serem encaixadas, subtrai-se 10% do peso bruto; c - Peso líquido (PL de frutas em caixas tipo torito (Cx (Cx = PL/ 18,85; d - Cento de frutas (CF em caixas tipo torito (Cx (Cx = CF/ 14.

  8. Cultivars

    Directory of Open Access Journals (Sweden)

    Oleg Blum

    2011-01-01

    Full Text Available Some modern agrochemicals with antioxidant potential were tested for their protective effect against ozone injury using clover and tobacco ozone-sensitive cultivars as model plants subjected to ambient ozone at two sites (Kyiv city in Ukraine and Szarów village in Poland. All used agrochemicals showed partial protective effects against ozone injury on clover and tobacco. Conducted studies confirmed the effectiveness of modern fungicides belonging to strobilurin group as protectants of sensitive crops against ozone damage. The effectiveness of new growth regulators “Emistym C” and “Agrostymulin” was showed for the first time. Out of the studied agrochemicals, fungicide “Strobi” and natural growth regulator “Emistym C” demonstrated the best protective effects. These agrochemicals present promise for further studies of their possible utilization for enhancement of ozone tolerance of sensitive crops.

  9. Host-induced post-transcriptional hairpin RNA-mediated gene silencing of vital fungal genes confers efficient resistance against Fusarium wilt in banana.

    Science.gov (United States)

    Ghag, Siddhesh B; Shekhawat, Upendra K S; Ganapathi, Thumballi R

    2014-06-01

    Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense (Foc), is among the most destructive diseases of banana (Musa spp.). Because no credible control measures are available, development of resistant cultivars through genetic engineering is the only option. We investigated whether intron hairpin RNA (ihpRNA)-mediated expression of small interfering RNAs (siRNAs) targeted against vital fungal genes (velvet and Fusarium transcription factor 1) in transgenic banana could achieve effective resistance against Foc. Partial sequences of these two genes were assembled as ihpRNAs in suitable binary vectors (ihpRNA-VEL and ihpRNA-FTF1) and transformed into embryogenic cell suspensions of banana cv. Rasthali by Agrobacterium-mediated genetic transformation. Eleven transformed lines derived from ihpRNA-VEL and twelve lines derived from ihpRNA-FTF1 were found to be free of external and internal symptoms of Foc after 6-week-long greenhouse bioassays. The five selected transgenic lines for each construct continued to resist Foc at 8 months postinoculation. Presence of specific siRNAs derived from the two ihpRNAs in transgenic banana plants was confirmed by Northern blotting and Illumina sequencing of small RNAs derived from the transgenic banana plants. The present study represents an important effort in proving that host-induced post-transcriptional ihpRNA-mediated gene silencing of vital fungal genes can confer efficient resistance against debilitating pathogens in crop plants.

  10. REGISTRO DE ESPÉCIES DE COLEOBROCAS ATACANDO BANANEIRAS DA CULTIVAR ‘TERRA’

    Directory of Open Access Journals (Sweden)

    SÔNIA MARIA FORTI BROGLIO

    2014-01-01

    Full Text Available It is reported the occurrence of coleoborers Cosmopolites sordidus (Germar, 1824, Metama- sius hemipterus (L., 1758, Metamasius canalipes (Gyllenhal, 1838 and Rhynchophorus palmarum (L., 1758 attacking banana cultivar 'Terra' (Musa paradisiaca (Musaceae, in the municipality of Joaquim Gomes, State of Alagoas. Twenty adult specimens were sent to Prof. Dr. Sergio Antonio Vanin, Department of Zoology, In- stitute of Biosciences, University of São Paulo and identified by morphological characteristics, being this the first report of M. canalipes and R. palmarum damaging the cultivar 'Terra’ in Brazil. Attack symptoms in the field are initially manifested by chlorosis, necrosis and fall of the lower leaves, the fruits did not develop satis- factorily and plants finished by fall over. As a result of infestation was observed the death of 70% of the plants evaluated in the area.

  11. Effect of coconut palm proximities and Musa spp. germplasm resistance to colonization by Raoiella indica (Acari: Tenuipalpidae)

    Science.gov (United States)

    Although coconut (Cocos nucifera L.) is the predominant host for Raoiella indica Hirst (Acari: Tenuipalpidae), false spider mite infestations do occur on bananas and plantains (Musa spp. Colla). Since its introduction, the banana and plantain industries have been negatively impacted to different deg...

  12. Caracterização da farinha de banana verde Green banana flour characterization

    Directory of Open Access Journals (Sweden)

    Antonia de Maria Borges

    2009-06-01

    Full Text Available O presente trabalho objetivou a obtenção, a caracterização físico-química e o controle microbiológico durante o processamento da farinha de banana (Musa spp. verde, cv. Prata, visando o seu aproveitamento na panificação, produtos dietéticos e alimentos infantis. Para obtenção da farinha, os frutos foram cortados, imersos em metabissulfito de sódio, desidratados e triturados, sendo em seguida, feitas as seguintes determinações: umidade; extrato etéreo; proteína bruta; fibra bruta; cinzas; fração glicídica; amido; valor calórico; pH; acidez total titulável; vitamina C; macrominerais (K, P, Ca, Mg, S e N; microminerais (B, Cu, Mn, Zn e Fe; coliformes a 45 °C; fungos filamentosos e leveduras; Bacillus cereus; Salmonella sp.; Staphylococcus aureus; e contagem de aeróbios mesófilos. Os resultados indicaram que a banana 'Prata' verde é viável para o processo de obtenção da farinha de banana, tendo em vista que é rica em amido, proteína, potássio, fósforo, magnésio, zinco, cobre e tem um alto valor calórico. O pH, a acidez total titulável e a vitamina C estão compatíveis com os valores encontrados em outras farinhas. Quanto ao uso de boas práticas no processamento, a farinha encontra-se dentro do padrão microbiológico ideal e, portanto, está apta para o consumo.The objective of the present study was the physicochemical characterization and the microbiological control during the processing of the green banana flour (Musa spp., Prata cultivar, aiming at the use of the flour in bread-making, dietary products and children's food. To obtain the flour, the fruits were cut, immersed in sodium meta-bisulfite, dehydrated, and ground. The following criteria were determined: humidity; ethereal extract; raw protein; raw fiber; ash; glicidic fraction; starch; caloric value; pH; total titratable acidity; vitamin C; macrominerals (K, P, Ca, Mg, S and N; microminerals (B, Cu, Mn, Zn and Fe; coliforms at 45 °C; filamentous

  13. High Frequency Plant Regeneration of Musa paradisiaca cv. Karibale Monthan

    Directory of Open Access Journals (Sweden)

    R. Shashi Kumar

    2015-06-01

    Full Text Available High frequency plant regeneration protocol has been standardized from banana cultivar Musa paradisiaca cv. Karibale Monthan, an endemic cultivar of Malnad region of Karnataka. The fruits are used as glomerular protective to solve kidney problems. To minimize the microbial contamination and to promote healthy growth, explants were treated with 70 % absolute alcohol for 6 min, 0.1 % Mercuric chloride for 10 min and 0.2 % for 10 min, 1 % Sodium hypochlorite for 15 min, 0.1 % Cefotaxime for 5 min and 0.05 % Gentamicin for 5 min. The high frequency shoot initiation (93.33 % was recorded at 5 mg/l BAP. The synergetic effect of BAP (4 to 6 mg/l, TDZ (0.1 to 1.2 mg/l and coconut water (0.1 to 0.9 ml/l induced multiple shoot buds and it was optimized at the concentration of 5 mg/l BAP, 0.5 mg/l TDZ and 0.5 ml/l coconut water with 15.90 ± 1.66 frequency of shoots per propagule. Supplementation of 1.0 mg/l IBA induced 5.33 ± 1.21 numbers of roots with a mean root length of 7.50 ± 1.87 roots. The 99% of plantlets with distinct roots and shoots were successfully acclimatized in the green house and transferred to the field to evaluate the agro-morphological variations. The weight of the bunch (kg, number of hands in a bunch, number of fingers in a hand, length of the finger (cm, girth of the finger (cm and girth of the pseudostem (cm exhibited by in vitro plants were higher than the in vivo plants.

  14. Ecophysiological and agronomic response of Abaca (Musa textilis) to different resource conditions in Leyte Island, Philippines

    OpenAIRE

    Bande, Marlito M.

    2012-01-01

    Abaca (Musa textilis Née) is closely related to edible bananas (Musa acuminata Colla and M. balbisiana Colla). Abaca usually thrives in the shade beneath tall trees, especially important for protecting the young plants from the sun and the older, taller plants from wind breakage. However, there is still disagreement on the need for shade trees in abaca cultivation. Hence, this study was conducted to ascertain the ecophysiological and agronomic response of abaca grown in different shade condit...

  15. Quantitative digital imaging of banana growth suppression by plant parasitic nematodes.

    Directory of Open Access Journals (Sweden)

    Hugh Roderick

    Full Text Available A digital camera fitted with a hemispherical lens was used to generate canopy leaf area index (LAI values for a banana (Musa spp. field trial with the aim of establishing a method for monitoring stresses on tall crop plants. The trial in Uganda consisted of two cultivars susceptible to nematodes, a plantain, Gonja manjaya and an East African Highland banana, Mbwazirume, plus a nematode resistant dessert banana, Yangambi km5. A comparative approach included adding a mixed population of Radopholus similis, Helicotylenchus multicinctus and Meloidogyne spp. to the soil around half the plants of each cultivar prior to field planting. Measurements of LAI were made fortnightly from 106 days post-planting over two successive cropping cycles. The highest mean LAI during the first cycle for Gonja manjaya was suppressed to 74.8±3.5% by the addition of nematodes, while for Mbwazirume the values were reduced to 71.1±1.9%. During the second cycle these values were 69.2±2.2% and 72.2±2.7%, respectively. Reductions in LAI values were validated as due to the biotic stress by assessing nematode numbers in roots and the necrosis they caused at each of two harvests and the relationship is described. Yield losses, including a component due to toppled plants, were 35.3% and 55.3% for Gonja manjaya and 31.4% and 55.8% for Mbwazirume, at first and second harvests respectively. Yangambi km5 showed no decrease in LAI and yield in the presence of nematodes at both harvests. LAI estimated by hemispherical photography provided a rapid basis for detecting biotic growth checks by nematodes on bananas, and demonstrated the potential of the approach for studies of growth checks to other tall crop plants caused by biotic or abiotic stresses.

  16. Quantitative digital imaging of banana growth suppression by plant parasitic nematodes.

    Science.gov (United States)

    Roderick, Hugh; Mbiru, Elvis; Coyne, Danny; Tripathi, Leena; Atkinson, Howard J

    2012-01-01

    A digital camera fitted with a hemispherical lens was used to generate canopy leaf area index (LAI) values for a banana (Musa spp.) field trial with the aim of establishing a method for monitoring stresses on tall crop plants. The trial in Uganda consisted of two cultivars susceptible to nematodes, a plantain, Gonja manjaya and an East African Highland banana, Mbwazirume, plus a nematode resistant dessert banana, Yangambi km5. A comparative approach included adding a mixed population of Radopholus similis, Helicotylenchus multicinctus and Meloidogyne spp. to the soil around half the plants of each cultivar prior to field planting. Measurements of LAI were made fortnightly from 106 days post-planting over two successive cropping cycles. The highest mean LAI during the first cycle for Gonja manjaya was suppressed to 74.8±3.5% by the addition of nematodes, while for Mbwazirume the values were reduced to 71.1±1.9%. During the second cycle these values were 69.2±2.2% and 72.2±2.7%, respectively. Reductions in LAI values were validated as due to the biotic stress by assessing nematode numbers in roots and the necrosis they caused at each of two harvests and the relationship is described. Yield losses, including a component due to toppled plants, were 35.3% and 55.3% for Gonja manjaya and 31.4% and 55.8% for Mbwazirume, at first and second harvests respectively. Yangambi km5 showed no decrease in LAI and yield in the presence of nematodes at both harvests. LAI estimated by hemispherical photography provided a rapid basis for detecting biotic growth checks by nematodes on bananas, and demonstrated the potential of the approach for studies of growth checks to other tall crop plants caused by biotic or abiotic stresses.

  17. Occurrence and Distribution of Banana bunchy top virus Related Agro-Ecosystem in South Western, Democratic Republic of Congo

    OpenAIRE

    Mukwa Fama Tongo, Lyna; Muengula, Marcel; Zinga, I; Kalonji, Adrien; Iskra-Caruana, M.L.; Bragard, Claude

    2014-01-01

    Banana bunchy top virus (BBTV) is one of the most severe and widespread virus limiting produc- tion and distribution of planting material of banana (Musa spp.) crops in the world. In Democratic Republic of Congo (DRC), these crops play a major role in daily life of almost 70% of citizen. Many factors influence banana production negatively such as Banana bunchy top disease. Epidemiol- ogical survey was conducted in experimental stations and farmers’ fields for two consecutive sea- sons coverin...

  18. Effect of mulching on banana weevil movement relative to pheromone traps

    NARCIS (Netherlands)

    Tinzaara, W.; Gold, C.S.; Dicke, M.; Huis, van A.; Ragama, P.E.

    2008-01-01

    Banana weevil (Cosmopolites sordidus) is a major pest in East Africa causing yield losses of up to 14 metric tonnes per hectare annually. A study was conducted in Uganda to determine the effect of mulching on banana (Musa spp. L.) weevil, Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae), m

  19. Phyllosticta species associated with freckle disease of banana

    NARCIS (Netherlands)

    Wong, M.H.; Crous, P.W.; Henderson, J.; Groenewald, J.Z.; Drenth, A.

    2012-01-01

    The identity of the casual agent of freckle disease of banana was investigated. The pathogen is generally referred to in literature under its teleomorphic name, Guignardia musae, or that of its purported anamorph, Phyllosticta musarum. Based on morphological and molecular data from a global set of b

  20. Impact of Diseases on Export and Smallholder Production of Banana

    NARCIS (Netherlands)

    Ploetz, R.C.; Kema, G.H.J.; Ma, Li Jun

    2015-01-01

    Banana (Musa spp.) is one of the world's most valuable primary agricultural commodities. Exported fruit are key commodities in several producing countries yet make up less than 15 of the total annual output of 145 million metric tons (MMT). Transnational exporters market fruit of the Cavendish cu

  1. Physiological and biochemical changes during banana ripening and finger drop

    NARCIS (Netherlands)

    Imsabai, W.; Ketsa, S.; Doorn, van W.G.

    2006-01-01

    Fruit drop of banana is due to breaking at the junction of the pedicel and pulp, and we found no true abscission zone. The breakage seems therefore due to weakening of the peel. We investigated pectin hydrolysis and some properties at the rupture zone, using `Hom Thong` (Musa acuminata, AAA Group) a

  2. Avaliação de variante somaclonal de porte baixo de bananeira 'Nanicão Jangada' (Musa sp em duas densidades Evaluation of a dwarf somaclonal variant of banana 'Nanicão Jangada' (Musa sp in two planting densities

    Directory of Open Access Journals (Sweden)

    Patricia da Costa Zonetti

    2003-12-01

    Full Text Available Avaliou-se, sob duas densidades de plantio, um variante somaclonal de porte baixo de bananeira, comparando-o com a variedade Nanicão Jangada que lhe deu origem. Os materiais genéticos 'Nanicão Jangada'(controle e o variante somaclonal representado pelas seleções 224 e 225 de um ensaio anterior, foram avaliados nos espaçamentos 2,0m X 2,0m (densidade 2500 plantas ha-1 e 3,0m X 2,0m (1666plantas ha-1, na Fazenda de Ensino e Pesquisa da Faculdade de Engenharia - UNESP, Campus de Ilha Solteira-SP. O ensaio foi conduzido em blocos ao acaso com cinco repetições, com utilização de mudas micropropagadas, sob irrigação por gotejamento, no período de dezembro de 1998 a março de 2001, com avaliação dos dois primeiros ciclos de produção. Constatou-se efeito da densidade e do ciclo sobre a produção estimada de frutos sendo que no cultivo mais denso, a média foi de 81,25 t.ha-1 no primeiro ciclo de produção e 67,93 t.ha-1 no segundo ciclo. No cultivo de menor densidade a produção estimada no primeiro ciclo foi de 51,35 t.ha-1 e 44,08 t.ha-1 no segundo. As seleções do variante de porte baixo apresentaram menor altura da planta e mostraram-se relativamente mais precoces e com produção semelhante a cv. Nanicão Jangada no primeiro ciclo. No segundo ciclo houve uma queda na produção do bananal, com maior intensidade para a seleção 225 do variante.A dwarf somaclonal variant of banana was evaluated under two planting densities. It was compared with the cultivar "Nanicão Jangada", the original clone from which the variant derived. The genotypes 'Nanicão Jangada'(control and the somaclone, represented by selections 224 and 225 from a previous experiment, were evaluated at spaced 2.0 m X 2.0 m (density of 2500 plants ha-1 and 3.0 m X 2.0 m (1666 plants ha-1 at the "Fazenda de Ensino e Pesquisa da Faculdade de Engenharia - UNESP", Campus de Ilha Solteira, SP. The experiment was conducted in randomized complete blocks with 5

  3. Severity of banana leaf spot in an intercropping system in two cycles of banana Prata Ana

    OpenAIRE

    Valdeir Dias Gonçalves; Silvia Nietsche; Marlon Cristian Toledo Pereira; Manoel Xavier de Oliveira Júnior; Roberto Célio Antunes Júnior; Carlos Ruggiero

    2008-01-01

    Prata Ana is the most planted banana cultivar in northern Minas Gerais, Brazil. It is however susceptible to several pathogens. This study was carried out to evaluate the disease severity of banana leaf spot in the Prata Ana cv. in the first and second cycle under six different planting systems. The randomized block experimental design was used with six treatments and four replications. lit an evaluation of the severity of banana leaf spot, no disease symptoms were found on Thap Maeo and Caip...

  4. Stomatal density and responsiveness of banana fruit stomates.

    Science.gov (United States)

    Johnson, B E; Brun, W A

    1966-01-01

    Determination of stomatal densities of the banana peel (Musa acuminata L. var Hort. Valery) by microscopic observations showed 30 times fewer stomates on fruit epidermis than found on the banana leaf. Observations also showed that peel stomates were not laid down in a linear pattern as on the leaf.It was demonstrated that stomatal responses occurred in banana fruit. Specific conditions of high humidity and light were necessary for stomatal opening: low humidity and darkness were necessary for closure. Responsiveness of the stomates continued for a considerable length of time after the fruit had been severed from the host.

  5. COMPONENTS OF CELL WALL, ENZYME ACTIVITY IN PEDICEL AND SUSCEPTIBILITY OF BANANAS TO FINGER DROP

    OpenAIRE

    GLORIA ANNABELL COBEÑA RUIZ; LUIZ CARLOS CHAMHUM SALOMÃO; DALMO LOPES DE SIQUEIRA; SEBASTIÃO TAVARES DE REZENDE; LEILA CRISTINA ROSA DE LINS

    2016-01-01

    ABSTRACT A major problem in post-harvest handling of bananas is the individual detachment of the fruit from the hands. This study aimed to establishing the relationship between carbohydrate concentration and enzyme activity in the pedicel region of three cultivars of bananas, resistant and susceptible to natural dropping, during post-harvest ripening, and the susceptibility of bananas to finger dropping. Cultivars ‘Terra’ (plantain, AAB group) and ‘Prata’ (banana, AAB group) triploids and th...

  6. EFEITOS DA RADIAÇÃO GAMA EM BANANA “NANICA” (MUSA SP., GRUPO AAA IRRADIADA NA FASE PRÉ-CLIMATÉRICA

    Directory of Open Access Journals (Sweden)

    S. F. SILVA

    2008-11-01

    Full Text Available

    O presente trabalho verifi cou os efeitos da radiação gama sobre parâmetros físicos e químicos da banana “nanica”, analisando possíveis alterações do período de conservação e a possibilidade de irradiação comercial visando à exportação. Os resultados demonstraram que as radiações não produziram efeito sobre o pH e acidez total. Porém, as bananas do grupo controle e aquelas que receberam dose de 0,75 kGy, apresentaram maior grau de maturação e as irradiadas com dose 0,30 kGy apresentaram maior fi rmeza. De acordo com os resultados da análise organoléptica, pode-se perceber que as bananas mais maduras, especialmente as do grupo controle, tiveram maior aceitação. As bananas dos tratamentos 0,30 e 0,60 kGy tiveram menores notas por apresentarem menor estádio de maturação. Sabendo-se que a irradiação em dose adequada e em frutos de boa qualidade traz benefícios ao armazenamento e ao processo de exportação, concluímos que a dose mais apropriada para o controle da maturação da banana “nanica” é a de 0,30 kGy.

  7. Changes in Homogalacturonans at Different Esterification Degrees during the Development of Banana (Musa spp .AAA) Leaves%香蕉叶片发育过程中不同甲酯化程度果胶的变化

    Institute of Scientific and Technical Information of China (English)

    马娟; 范韦; 李小泉; 陈厚彬; 谢玲; 刘静; 吴茜; 袁梦; 徐春香

    2016-01-01

    采用免疫荧光标记技术,利用5种识别不同甲酯化程度聚半乳糖醛酸(HGs )果胶及香蕉果胶甲酯酶(PM E)的单克隆抗体,对不同株龄香蕉叶片中PM E及不同甲酯化程度的HGs定位、相对含量以及PM E活性的变化进行分析,为探讨HGs和PME在香蕉生长发育及抵抗逆境过程中的功能和生理机制奠定基础。结果显示:(1) PM E主要在组培苗的叶肉和保卫细胞中表达,其表达量及其酶的活性均随着香蕉株龄的增长而呈现下降趋势。(2)叶肉细胞也是各类不同甲酯化程度 HGs的主要分布区域,其含量随着香蕉株龄的增长有不同程度的下降,但不同HGs在叶肉细胞中的含量以及在表皮、保卫细胞及叶脉中的分布模式不尽相同,保卫细胞中 HGs的甲酯化程度较高。研究表明,香蕉的叶肉细胞是PM E及这5种不同甲酯化程度HGs的主要分布场所,且这些 HGs在香蕉发育过程中的含量变化趋势与PM E相似。%For better understanding the functions and mechanism of PMEs and HGs in the growth and de-velopment of banana and in the defense of banana to the stresses ,we traced the spatial and temporal abun-dance and distribution of banana pectin methylesterases (PM Es) and five homogalacturonans (HGs) at dif-ferent methyl-esterification degrees (MDs) in banana leaves at different developmental stages using immu-no-fluorescence labeling techniques .In addition ,enzyme activity of PMEs was also tested .The results in-dicated that :(1) PMEs located mainly in the mesophyll and guard cells in tissue-cultured banana seed-lings .Both the level and activity of PMEs decreased with the development of the plant .(2) HGs at differ-ent MDs also mainly appeared in mesophyll cells in tissue-cultured plants and their contents reduced gradu-ally with the development of plants ,though the speed varied with each other .However ,the distribution of these HGs in the epidermis ,the guard

  8. Avaliação das cultivares de bananeira Prata-Anã, Thap Maeo e Caipira em diferentes sistemas de plantio no norte de Minas Gerais Evaluation of Prata Anã, Thap Maeo and Caipira banana cultivars in different planting systems in the north of Minas Gerais

    Directory of Open Access Journals (Sweden)

    Valdeir Dias Gonçalves

    2008-06-01

    Full Text Available A banana é uma das frutas tropicais mais consumidas no mundo, respondendo por, aproximadamente, 10% do comércio mundial de frutas. O objetivo deste trabalho foi avaliar o crescimento e a produção de três cultivares de bananeira sob três sistemas de plantio, em dois ciclos de produção, no período compreendido entre março de 2004 e outubro de 2006, em Janaúba, Minas Gerais. O delineamento experimental foi o de blocos casualizados e duas análises estatísticas: Primeira: 'Prata-Anã' em seis diferentes sistemas de plantio; Segunda: fatorial 2 x 2 + 1, com as variedades Caipira e Thap Maeo, em três diferentes sistemas de plantio, com quatro repetições e seis plantas úteis por parcela. Foram avaliadas as seguintes características: altura de planta, circunferência do pseudocaule, número total de folhas, número de folhas vivas na colheita, número de dias do plantio à colheita, massa do cacho, produtividade, número de pencas e número de frutos por cacho. As características avaliadas foram submetidas à análise de variância com desdobramentos das interações significativas, tendo os efeitos dos tratamentos comparados pelo teste de Tukey, a 5 % de probabilidade. A mistura de cultivares e o uso de bordaduras não influenciaram na maioria das características de crescimento, nas três cultivares estudadas. A cultivar Thap Maeo foi superior à 'Caipira' na maioria das características avaliadas, no primeiro e segundo ciclos. Para os sistemas de plantio, foi superior o plantio com uma bordadura. A análise da variedade Prata-Anã dentro dos seis sistemas de plantio apresentou diferença significativa para as características de número de folhas vivas na colheita, no segundo ciclo, número de pencas/cacho e número de frutos/cacho.Banana is one of the tropical fruits more consumed in the world, answering for approximately 10% of the world trade of fruits. The purpose of this work was to evaluate the growth and production of three

  9. 香蕉枯萎病抗性品种及绿色药剂的初步筛选%Preliminary screening of cultivars and green fungicides against banana Fusarium wilt

    Institute of Scientific and Technical Information of China (English)

    杨静美; 冯岩; 林铭欢; 伍惠媚; 黄秉智; 许林兵

    2014-01-01

    为筛选抗枯萎病香蕉品种,通过伤根、针刺及淋菌法接种香蕉枯萎病菌4号小种,进行盆栽试验,结果表明,品种‘巴西蕉’和‘南天红’的病情指数较高,分别达42.5和37.5,为中感品种;而品种‘南天青’和‘南天黄’病情指数仅为15,为抗病品种;‘农科一号’病情指数最低,仅为7.5,是5个品种中最抗病的,为高抗品种。为筛选防治香蕉枯萎病的绿色药剂,选用壳聚糖水剂、竹醋液、耕保泰对比络氨铜水剂对‘巴西蕉’进行盆栽药效试验,结果表明,耕保泰防治效果较好,相对防效达78.57%;20%竹醋液的防治效果最差,仅为35.71%。%In order to select cultivars against banana Fusarium wilt,pot experiments were carried out with the in-oculation methods of root cutting,prodding and sprinkling fungus of Fusarium oxysporum f.sp.cubense Race 4. The results showed that after inoculated with Fusarium ,the disease indexes of ‘Brazil banana’and ‘Skyred ba-nana’were 42.5 and 37.5,respectively,indicating they were middle susceptible.While the disease index of‘Skygreen’was 15,the same as ‘Skyyellow’,indicating they were the resistant cultivars.‘Agricultural one’dem-onstrated the lowest disease index of all the cultivars,only 7.5,which was the high resistant cultivar.Green fungicides against banana Fusarium wilt were also screened by pot experiments with chitosan,bamboo vinegar,Gengbaotai,com-pared with cuaminosulfate.The pot experiment with fungicides on ‘Brazil banana’showed that Gengbaotai had the best control effiacy by 78.57%.However,20% of bamboo vinegar was the weakest with the efficacy of 35.71%.

  10. Antifungal Activities of Crude Extractum from Camellia semiserrata Chi (Nanshancha) Seed Cake Against Colletotrichum musae, Colletotrichum gloeosporioides and Penicillium italicum in vitro and in vivo Fruit Test.

    Science.gov (United States)

    Meng, Xiangchun; Li, Jun; Bi, Fangcheng; Zhu, Lixue; Ma, Zhiyu

    2015-12-01

    Antifungal activities of crude extractum of Nanshancha Seed Cake (NSC), to inactivate postharvest pathogens were investigated. Highest inhibitory rate was found against C. musae, C. gloeosporioides and C. papaya P.Henn, which was much stronger than that by tea saponin. Compared to tea saponin, effects of NSC extractum was relatively weak and similar on C. gloeosporioides Penzig and P. italicum. In an in vivo study, best controlling effects by NSC extractum was found with banana anthracnose disease development, which showed no inhibitory effects by tea saponin. NSC extractum controlled in vitro C. musae growth through directly inhibiting germination rate and germ tube elongation, and causing distortation, rupture and indentation of C. musae mycelium. In banana fruit subject to C. musae inoculation, higher PAL, POD, GLU and CHT activity was observed in banana fruit treated with crude NSC extractum than that of water control fruits. Current study proved the best controlling effects of crude NSC extractum in C. musae in vitro and in vivo development, which through direct inhibition of C. musae growth and increasing defense system of the banana fruit.

  11. Antifungal Activities of Crude Extractum from Camellia semiserrata Chi (Nanshancha Seed Cake Against Colletotrichum musae, Colletotrichum gloeosporioides and Penicillium italicum in vitro and in vivo Fruit Test

    Directory of Open Access Journals (Sweden)

    Xiangchun Meng

    2015-12-01

    Full Text Available Antifungal activities of crude extractum of Nanshancha Seed Cake (NSC, to inactivate postharvest pathogens were investigated. Highest inhibitory rate was found against C. musae, C. gloeosporioides and C. papaya P.Henn, which was much stronger than that by tea saponin. Compared to tea saponin, effects of NSC extractum was relatively weak and similar on C. gloeosporioides Penzig and P. italicum. In an in vivo study, best controlling effects by NSC extractum was found with banana anthracnose disease development, which showed no inhibitory effects by tea saponin. NSC extractum controlled in vitro C. musae growth through directly inhibiting germination rate and germ tube elongation, and causing distortation, rupture and indentation of C. musae mycelium. In banana fruit subject to C. musae inoculation, higher PAL, POD, GLU and CHT activity was observed in banana fruit treated with crude NSC extractum than that of water control fruits. Current study proved the best controlling effects of crude NSC extractum in C. musae in vitro and in vivo development, which through direct inhibition of C. musae growth and increasing defense system of the banana fruit.

  12. PINZOTE de Musa balbisiana y Musa acuminata como Fuente de Fibras para Papel RACHIS of Muse balbisiana and Muse acuminata as Source of Fibers for Paper Structure

    Directory of Open Access Journals (Sweden)

    José Turrado

    2009-01-01

    Full Text Available Este trabajo presenta una alternativa de uso industrial del pinzote (centro leñoso del racimo de banano como fuente de fibra para la estructuración de papel. El pinzote se considera residuo agroindustrial en los países productores de banano, material que representa el 2.8 % del racimo en la variedad de Musa balbisiana y el 4.4 % en la de Musa acuminata. Por sus características similares a la del bagazo caña de azúcar, se utilizó un proceso químico similar al empleado para obtener fibras a partir de este material. El proceso de obtención de fibras se llevó a cabo de acuerdo a un diseño experimental factorial, diseño que permite relacionar variables las independientes (tiempo de cocción, concentración de reactivo en cuatro niveles con variables independientes (rendimiento, consumo de reactivos, rechazo y lignina residual. El pinzote de Musa acuminata requiere menor temperatura y reactivo para liberar las fibras con respecto al pinzote de Musa balbisiana. Por lo tanto, el rendimiento en fibra para Musa acuminata es mayor al de Musa balbisiana.This work presents an alternative of industrial use of the banana rachis (the woody center of the cluster of bananas, as source of fibers for the structure of paper. Banana rachis is considered to be an agroindustrial residue in producing countries of banana it represents 2.8 % of the cluster of Muse balbisiana and 4.4 % of the cluster of Muse acuminata. The physical and chemical structure of the rachis is almost simliar to that of the sugar cane so a chemical process similar to that employed to obtain fibers from the sugar bagasse cane was used. An experimental factorial design that allows the correlation of independent variables (cooking time, reagent concentration in four levels with response parameters (yield, reagents consumption, rejects and residual lignin was adopted. The rachis of Muse acuminata needs lower temperature and less reagent consumption to liberate fibers in comparison with

  13. Avaliação de cultivares e híbridos de bananeira em quatro ciclos de produção Evaluation of banana cultivars and hybrids in four production cycles

    Directory of Open Access Journals (Sweden)

    Sebastião de Oliveira e Silva

    2002-11-01

    Full Text Available Híbridos superiores de bananeira com alta produtividade, frutos vistosos e resistência a doenças estão sendo gerados no programa de melhoramento genético da Embrapa-Centro Nacional de Pesquisa de Mandioca e Fruticultura Tropical. Este trabalho objetivou avaliar alguns desses genótipos em quatro ciclos de produção, visando sua recomendação aos agricultores. O experimento foi realizado em delineamento inteiramente casualizado, com 18 repetições, em Cruz das Almas, BA. Avaliaram-se as cultivares Grande-Naine, Nanica, Nam, Thap Maeo, Mysore, Caipira, Prata-Comum, Pacovan e Prata-Anã e os híbridos Pioneira, PA03-22, FHIA-18, PV03-76, PV03-44 e JV03-15. Analisaram-se os caracteres altura da planta, diâmetro do pseudocaule, peso do cacho, número de frutos, comprimento do fruto e ciclo. Ao longo dos ciclos, a 'Nanica' apresentou o menor porte, enquanto a 'Prata-Anã' se destacou no diâmetro do pseudocaule, seguida pela 'Prata-Comum' e pelo JV03-15. A 'Thap Maeo' sobressaiu-se no peso do cacho e no número de frutos, seguida pelo FHIA-18, no primeiro caráter, e pela 'Mysore', pela 'Caipira' e pelo FHIA-18, no segundo caráter. A 'GrandeNaine' e a 'Nanica' apresentaram o maior comprimento do fruto, enquanto o FHIA-18 e o PA03-22 destacaram-se na precocidade. A 'Thap Maeo' tem potencial para substituir a 'Mysore' e os híbridos avaliados apresentam qualidade para vir a ser recomendados aos agricultores.New productive banana hybrids, presenting good fruits and disease resistant, have been generated at Embrapa-Centro Nacional de Pesquisa de Mandioca e Fruticultura Tropical. The objective of this work was to evaluate some of these genotypes in four production cycles, to recommend them to the farmers. The experiment was carried out in the completely randomized design with 18 replications in Cruz das Almas, BA, Brazil. The genotypes evaluated were the varieties Grande-Naine, Nanica, Nam, Thap Maeo, Mysore, Caipira, Prata-Comum, Pacovan and

  14. First report offusarium oxysporumf. sp.cubensetropical race 4 causing panama disease in cavendish bananas in Pakistan and Lebanon

    NARCIS (Netherlands)

    Ordoñez, N.; García-Bastidas, F.; Laghari, H.B.; Akkary, M.Y.; Harfouche, E.N.; Awar, al B.N.; Kema, G.H.J.

    2016-01-01

    Panama disease of banana, caused byFusarium oxysporumf. sp.cubense(Foc), poses a great risk to global banana production. Tropical race 4 (TR4) of Foc, which affects Cavendish bananas as well as many other banana cultivars (Ploetz 2006), was confirmed for the first time outside Southeast Asia in Jord

  15. 大蕉后熟期的褐变相关性及褐变底物鉴定%Correlations between Browning Degree and PPO or POD Activity of Dajiao BananaMusa paradisiaca ABB cv Dajiao) and Preliminary Identification of Browning Substrates during Postharvest Ripening

    Institute of Scientific and Technical Information of China (English)

    白永亮; 余铭; 袁根良; 杜冰; 鲁旺旺; 黄守耀; 杨公明

    2012-01-01

    The variation regularity of browning degree (BD), polyphenol oxidase (PPO) and peroxidase (POD) of Dajiao bananaMusa paradisiaca ABB cv. Dajiao) was observed and compared with other two banana varieties, Fenjiao and Cavendish. Meanwhile, the role of total and free phenol contents in the browning of Dajiao banana was investigated. BD indicated a progressively increasing trend in the three varieties of banana during postharvest ripening, and Fenjiao and Cavendish browned significantly faster than Dajian after the 13^th day of storage, suggesting that Dajiao was more suitable for deep processing. In addition, Dajiao exhibited low PPO and POD activities and low free phenol content but high total phenol content and consequently browned slowly, demonstrating an association between the BD and PPO or POD activities and a negative correlation between the browning rate and free content of The correlation coefficients between BD and PPO POD activities phenol Dajiao. or were 0.8789 and 0.9240, respectively. Thus, there is a close correlation between the browning potential and PPO or POD activities of Dajiao. The main browning substrates in Dajiao were gallic acid and epicatechin as preliminarily identified using UV-VIS absorption spectroscopy and HPLC.%以粉蕉和香牙蕉为对照,观察大蕉后熟过程中褐变度、多酚氧化酶和过氧化物酶的变化规律,同时考察大蕉总酚和游离酚含量分别在大蕉褐变过程中起的作用。结果表明:三种香蕉在后熟过程中,褐变度均呈逐渐增长趋势,在储藏13d后,粉蕉与香牙蕉的褐变速率明显高于大蕉,说明大蕉更适合于作为深加工的原料。在储藏13d后,大蕉的多酚氧化酶和过氧化物酶比活力均比较低,游离酚含量也比较低,但总酚含量较高,最终导致褐变速率较慢,说明大蕉的褐变度同多酚氧化酶和过氧化物酶有一定的相关性,大蕉的褐变速率与游离酚的含量呈

  16. FORMULASI TABLET SERBUK PISANG RAJA (Musa xparadisiaca AAB SEBAGAI PENUTUP TUKAK LAMBUNG PADA TIKUS

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    Iis Wahyuningsih

    2012-05-01

    Full Text Available Mucoadhesive is a topic of current interest in the design of drug delivery system and treatment of peptic ulcers. This concept is based on the strength of adhesion of a protective material to the mucosa. King Banana (Musa xparadisiaca AAB is often used in the treatment of abdominal pain. It has been suspected having muchoadhesive strength to cover peptic ulcer. The study was armed to formulate king banana tablet which has muchoadhesive ability to protect gastric mucosa become ulcer. The aim of peptic ulcer cover test was to see the ability of king banana tablet to covered ulcer at the groups test that received different treatment of the negative control group (solvent, the banana tablet suspension group, and sucralfate group. The ulcer severity was determined by scoring method. All experimental data were tested by SPSS analytical statistics. The result showed that king banana tablet has similar strength to peptic ulcer cover of sucralfat and not significantly different.

  17. Caracterização morfológica e avaliação de cultivares de bananeira resistentes a Sigatoka Negra (Mycosphaerella fijiensis Morelet no sudoeste goiano Morphologic characterization and evaluation of banana cultivars resistant to the Black Sigatoka disease (Mycosphaerella fijiensis Morelet in the Southwest of the State of Goiás, Brazil

    Directory of Open Access Journals (Sweden)

    Silvia Correa Santos

    2006-12-01

    Full Text Available Cinco cultivares de bananeira resistentes a Sigatoka Negra foram cultivadas na área experimental da Universidade Federal de Goiás, no município de Jataí, localizado na região Sudoeste do Estado. O objetivo foi introduzir genótipos resistentes a Sigatoka Negra no Estado, bem como fazer a caracterização morfológica desses materiais nessas condições climáticas. O trabalho foi realizado por meio da avaliação, em três ciclos produtivos, dos seguintes componentes de produção: intervalo em dias entre o plantio e o florescimento, número de folhas no florescimento e na colheita, número de pencas por cacho, frutos por cacho e frutos por penca, comprimento do cacho e dos frutos e peso do engaço, das pencas e do cacho. As cultivares falsa FHIA-18 e Caipira produziram em média aos 393 dias, apresentando maior precocidade em relação às demais cultivares. Como era esperado, a FHIA-21, que é uma bananeira tipo Terra, apresentou um ciclo maior (488 dias. O clima e a altitude de Jataí-GO podem ter contribuído para o aumento do ciclo das cinco cultivares quando comparado com outras regiões. Para as características de produção, a FHIA-01 e a falsa FHIA-18 se destacaram das demais. Para o número de pencas, a Thap Maeo se destacou nos três ciclos, mostrando a característica deste genótipo. As maiores alturas na floração foram observadas nas cultivares FHIA-21 e Thap Maeo, que também foram as que mais sofreram com os efeitos do vento.Five banana cultivars resistant to the Black Sigatoka Disease were grown at the experimental field of the Universidade Federal de Goiás (UFG, located in the Southwest region of the State of Goiás (Brazil. This work aimed the introduction of Black Sigatoka resistant cultivars as well as the characterization of the morphological aspects of such cultivars at that climatic region. The following characteristics were evaluated, in the first three cycles: days between planting and flowering, leaf number at

  18. 健康香蕉(Musa paradisiaca)植株与枯萎病患病植株根区土壤细菌多样性的比较研究%Comparison of Soil Bacterial Genetic Diversity in Root Zone of Banana(Musa paradisiaca) Infected with Fusarium Wilt and Non-Infected Plants

    Institute of Scientific and Technical Information of China (English)

    邓晓; 李勤奋; 武春媛; 李怡; 刘景坤

    2015-01-01

    Currently banana fusarium wilt (Fusarium oysporumf. cubense) is one of the important soil-borne diseases of seriously threat to banana production. However, there is no effective method for prevention in the world. The objective of this study was providing research information for the prevention of banana fusarium wilt from the perspective of microbial ecology. Two gene libraries of 16S rRNA were constructed by directly extracted from soil total DNA. And the soil samples were collected from root zone of banana which infected with fusarium wilt and non-infected plants in three typical plots infected by banana fusarium wilt (Jianfeng, Shiyuetian, Chongpo) in Hainan Province. The soil bacterial genetic diversity was analyzed by DNA sequencing and phylogenetic characters. The results were shown as follows: (1) the number of operational taxonomic units (OTUs) in soils from root zone of banana which infected with fusarium wilt was 18 lower than that of non-infected plants. The Shannon index (H') and calculated species richness (Schao1) in soils from root zone of banana which infected with fusarium wilt was respectively 0.66 and 26 lower than that of non-infected plants. (2) 63 OTUs were belonged to 9 bacterial taxa and 4 non-determined genotypes in soils from non-infected plants, and 45 OTUs were belonged to 8 bacterial taxa and 2 non-determined genotypes in soils from fusarium wilt infected plants. And the 6 bacterial phyla of Firmicutes, Proteobacteria, Actinobacteria, Acidobacteria, Gemmatimonadetes and Planctomycetes among them were common bacterial taxa in soils from root zone of banana both infected with fusarium wilt and non-infected plants. However, Cyanobacteria and Bacteria intertae sedis were only existed in the soils from root zone of banana plants which non-infected with fusarium wilt. In contrast, Nitrospia was only observed in the soils from root zone of infected plants. (3) The proportion of Firmicutes in soils from fusarium wilt infected plants was 11

  19. Vegetative and productive aspects of organically grown coffee cultivars under shaded and unshaded systems

    Directory of Open Access Journals (Sweden)

    Marta dos Santos Freire Ricci

    2011-08-01

    Full Text Available Although Coffea arabica species has its origin in the African understories, there is great resistance on the part of the Brazilian producers for growing this species under agroforestry systems as they fear that shading reduces production. This study aimed at evaluating some vegetative traits and the productivity of organically grown coffee (Coffea arabica L. cultivars under shaded and unshaded systems. Twelve treatments consisting of two cultivation systems (shaded and unshaded and six coffee cultivars were arranged in randomized blocks with four replicates, in a split-plot scheme. Shading was provided by banana (Musa sp. and coral bean plants (Erythrinaverna. Shading delayed fruit maturation. Late maturation cultivars, such as the Icatu and the Obatã, matured early in both cultivation systems, while medium and early maturation cultivars presented late maturation. Cultivation in the shaded system increased the leaf area and the number of lower branches, decreased the number of productive nodes per branch, and increased the distance between the nodes and the number of leaves present in the branches. Cultivation in the unshaded system presented greater number of plants with branch blight in relation to plants grown in the shade. The productivity of the cultivars was not different, at 30.0 processed bags per hectare in the shaded system, and 25.8 processed bags per hectare in the unshaded system. The most productive cultivars in the shaded system were the Tupi, the Obatã, and the Catuaí, while no differences between cultivars were obtained in the unshaded system.

  20. Banana Cakes

    Institute of Scientific and Technical Information of China (English)

    1998-01-01

    Ingredients: Two bananas, 50 grams of preserved fruits, 25 grams sesame seeds, 10 grams glutinous rice powder,white sugar,oil. Method: 1. Chop the preserved fruits and mix them well with vegetable oil. white sugar and sesame. 2. Mash the bananas into a paste and mix it with

  1. Effect of coconut palm proximities and Musa spp. germplasm resistance to colonization by Raoiella indica (Acari: Tenuipalpidae).

    Science.gov (United States)

    Rodrigues, Jose Carlos Verle; Irish, Brian M

    2012-08-01

    Although coconut (Cocos nucifera L.) is the predominant host for Raoiella indica Hirst (Acari: Tenuipalpidae), false spider mite infestations do occur on bananas and plantains (Musa spp. Colla). Since its introduction, the banana and plantain industries have been negatively impacted to different degrees by R. indica infestation throughout the Caribbean. Genetic resistance in the host and the proximity of natural sources of mite infestation has been suggested as two of the main factors affecting R. indica densities in Musa spp. plantations. Greenhouse experiments were established to try to determine what effect coconut palm proximities and planting densities had on R. indica populations infesting Musa spp. plants. Trials were carried out using potted Musa spp. and coconut palms plants at two different ratios. In addition, fourteen Musa spp. hybrid accessions were evaluated for their susceptibility/resistance to colonization by R. indica populations. Differences were observed for mite population buildup for both the density and germplasm accession evaluations. These results have potential implications on how this important pest can be managed on essential agricultural commodities such as bananas and plantains.

  2. Banana MaMADS transcription factors are necessary for fruit ripening and molecular tools to promote shelf-life and food security

    Science.gov (United States)

    Genetic solutions to postharvest crop loss can reduce cost and energy inputs while increasing food security, especially for banana (Musa acuminata), which is a significant component of worldwide food commerce. We have functionally characterized two banana E class (SEPALLATA3 [SEP3]) MADS box genes, ...

  3. Colonização micorrízica e concentração de nutrientes em três cultivares de bananeiras em um latossolo amarelo da Amazônia central Arbuscular mycorrhizal colonization and nutrient concentration of three cultivars of banana on a central Amazonian oxisol

    Directory of Open Access Journals (Sweden)

    Arlem Nascimento de Oliveira

    2003-01-01

    regional producers. Adaptation can be related to arbuscular mycorrhizae, that can increase the plant's capacity to absorb soil nutrients. This study was carried out in a banana plantation on a yellow Oxisol in the Agrarian Sciences Faculty (University of Amazonas Foundation, to verify the mycorrhizal colonization and the plant's nutrient status in the banana cultivars Maçã, Pacovan and Prata, during three months of evaluations (December/98, January and February/99. Samples of roots were collected to evaluate the rates of mycorrhizal colonization and leaves to verify the macro and micronutrient concentrations. The average of mycorrhizal colonization were 60.7% in the cultivar Maçã, 55.2% in Pacovan and 53.6% in Prata. Sampling done in December 1998 showed that the cultivar Maçã had lower fungal colonization (48.3% of the roots than Pacovan (73.6% and Prata (67.8%. In January 1999 the situation was inverted: Maçã presented the highest colonization (75.3% when compared with Pacovan (47.8% and Prata (40.3%. No difference in P and Fe concentrations was observed among cultivars, but there was significant variation among them for Ca, Mg, K, Zn, Cu and Mn. The mycorrhizal colonization was correlated positively with Ca, K and Zn in the cultivar Maçã, and Cu in Prata. These positive correlations allow us to infer that the mycorrhizal association was important to stimulate Ca, K and Zn absorption in the cultivar Maçã, and Cu in Prata in the commercial production stage of five years old banana trees.

  4. PROPOLIS EXTRACT IN POSTHARVEST CONSERVATION BANANA ' PRATA'

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    FLÁVIA REGINA PASSOS

    2016-01-01

    Full Text Available ABSTRACT In the present work were evaluated the effects of propolis coatings of various botanical sources on quality traits of bananas cv. Prata (Musa sapientum L. stored at room temperature. ´Prata´ bananas were selected and submitted to five postharvest treatments: four coatings applied by immersion in propolis extracts at a concentration of 2.5% (w/v and a control (without coating. Propolis extracts were applied as 1 a wild type aqueous propolis extract, 2 a wild type hydroalcoholic propolis extract, 3 a rosemary green type hydroalcoholic propolis extract and 4 a red type hydroalcoholic propolis extract. The bananas were evaluated at three-day intervals along 12 days for fresh weight losses, flesh firmness, soluble solids (SS, titratable acidity (TA, the ratio SS/TA and pH. Sensory analyses were performed after three and six days of storage by 55 not trained panelists designed for acceptability. At the end of the twelve-day storage period, bananas coated either with the rosemary green hydroalcoholic extract or with the aqueous extract presented lower fresh weight losses in comparison to the bananas of the control treatment. No differences were determined in relation to flesh firmness and along the storage period TA values decreased and pH values increased in bananas of all treatments. SS contents increased towards the end of the storage period that, consequently, contributed to increases in the SS/TA ratio. The most significant increase in SS/TA ratio was determined in bananas coated with the red type hydroalcoholic extract. Taste panelists did not detect significant differences amongst coated and not coated cv. Prata bananas up to six days of storage.

  5. Analysis of non-TIR NBS-LRR resistance gene analogs in Musa acuminata Colla: Isolation, RFLP marker development, and physical mapping

    Directory of Open Access Journals (Sweden)

    Souza Manoel T

    2008-01-01

    Full Text Available Abstract Background Many commercial banana varieties lack sources of resistance to pests and diseases, as a consequence of sterility and narrow genetic background. Fertile wild relatives, by contrast, possess greater variability and represent potential sources of disease resistance genes (R-genes. The largest known family of plant R-genes encode proteins with nucleotide-binding site (NBS and C-terminal leucine-rich repeat (LRR domains. Conserved motifs in such genes in diverse plant species offer a means for isolation of candidate genes in banana which may be involved in plant defence. Results A computational strategy was developed for unbiased conserved motif discovery in NBS and LRR domains in R-genes and homologues in monocotyledonous plant species. Degenerate PCR primers targeting conserved motifs were tested on the wild cultivar Musa acuminata subsp. burmannicoides, var. Calcutta 4, which is resistant to a number of fungal pathogens and nematodes. One hundred and seventy four resistance gene analogs (RGAs were amplified and assembled into 52 contiguous sequences. Motifs present were typical of the non-TIR NBS-LRR RGA subfamily. A phylogenetic analysis of deduced amino-acid sequences for 33 RGAs with contiguous open reading frames (ORFs, together with RGAs from Arabidopsis thaliana and Oryza sativa, grouped most Musa RGAs within monocotyledon-specific clades. RFLP-RGA markers were developed, with 12 displaying distinct polymorphisms in parentals and F1 progeny of a diploid M. acuminata mapping population. Eighty eight BAC clones were identified in M. acuminata Calcutta 4, M. acuminata Grande Naine, and M. balbisiana Pisang Klutuk Wulung BAC libraries when hybridized to two RGA probes. Multiple copy RGAs were common within BAC clones, potentially representing variation reservoirs for evolution of new R-gene specificities. Conclusion This is the first large scale analysis of NBS-LRR RGAs in M. acuminata Calcutta 4. Contig sequences were

  6. Banana technology

    Science.gov (United States)

    van Amstel, Willem D.; Schellekens, E. P. A.; Walravens, C.; Wijlaars, A. P. F.

    1999-09-01

    With 'Banana Technology' an unconventional hybrid fabrication technology is indicated for the production of very large parabolic and hyperbolic cylindrical mirror systems. The banana technology uses elastic bending of very large and thin glass substrates and fixation onto NC milled metal moulds. This technology has matured during the last twenty years for the manufacturing of large telecentric flat-bed scanners. Two construction types, called 'internal banana' and 'external banana; are presented. Optical figure quality requirements in terms of slope and curvature deviations are discussed. Measurements of these optical specifications by means of a 'finishing rod' type of scanning deflectometer or slope tester are presented. Design constraints for bending glass and the advantages of a new process will be discussed.

  7. Cooking Banana Consumption Patterns in the Plantain-growing Area of Southeastern Nigeria

    OpenAIRE

    Tshiunza, M.; Lemchi, J.; Onyeka, U.; Tenkouano, A.

    2001-01-01

    Cooking bananas (Musa spp., ABB genome) were intro-duced into Southeastern Nigeria by the International Institute of Tropical Agriculture (IITA) in the mid-1980s as an interim measure to reduce the incidence of black sigatoka disease (caused by the fungus Mycosphaerel-la fijiensis Morelet) on plantain. However, the people of this region were not familiar with their utilisation methods. To address this lack of the knowledge and thereby sustain cooking banana cultivation, IITA, in collaboration...

  8. Musa chunii Hakkinen, a new species (Musaceae) from Yunnan,China and taxonomic identity of Musa rubra

    Institute of Scientific and Technical Information of China (English)

    Markku H(A)KKINEN

    2009-01-01

    The center of diversity of the genus Musa (Musaceae) is in Southeast Asia, a region not studied in detail and where new species and varieties continue to be reported. A new wild banana species, M. chunii Hakki-nen from Yunnan, China is described and illustrated based on observed morphological characteristics in the field. This extremely rare new species was only found in Tongbiguan Nature Reserve, Dehong District, West Yunnan. A key to M. chunii and related taxa is provided. In addition, critical notes regarding M. rubra Kurz identity are given.

  9. ESTUDO DA CORRELAÇÃO DOS TEORES DE P, K, Al, Ca e Mg NO SOLO, COM A ALTURA DE BANANEIRAS DO CULTIVAR “MARMELO”, NA EMISSÃO DO CACHO P, K, Al, Ca, AND Mg SOIL CORRELATION STUDY WITH BANANA PLANT HEIGHT CULTIVAR "MARMELO" IN THE BUNCH EMISSION

    Directory of Open Access Journals (Sweden)

    Manoel Passos de Castro

    2007-09-01

    Full Text Available

    A bananeira do cultivar “Marmelo” vem, a cada dia, sendo a melhor opção para os bananicultores, que fornecem frutas às fábricas de doces. Com o aumento dos plantios deste cultivar, necessitou-se de dados concretos, na indicação de glebas, para a formação de bananais, tomando como base a análise do solo. Para isto tomaram-se 40 amostras, constituídas cada uma de 12 sub-amostras, ao redor de 40 touceiras de bananas. Estas touceiras foram escolhidas ao acaso, em um bananal de 2,5 ha, plantado no espaçamento de 3m x 3m, com idade de três anos. Para cada amostra analisaram-se os teores de P, K, Ca + Mg e Al, sendo estes correlacionados com a altura da bananeira correspondente, na emissão da inflorescência. Com base nas análises estatísticas concluiu-se que: a. Em caso de não se indicar adubação, o plantio só deve ser efetuado em solos com mais de 50 ppm. de K; b. A indicação de adubos fosfatados deverá ser feita apenas quando o teor de P no solo for menor que 1 ppm; c. O teor de Ca + Mg do solo deverá ser elevado para 2 (dois mE/100 ml, utilizando-se calcário dolomítico; d. O alumínio tóxico do solo deve ser totalmente neutralizado.

    For commercial banana production, flat fersain is preferable because farm implements and heavy machinery can move early in the fields, but in Goiás, flat land is generally of very low fertility. This study was undertaken to determine the growth of the “Marmelo” banana in this kind of poor soil, using correlations between analyses of soils and the heights of pseudo stems at flowering, and to suggest viable fertilization for its successful growth.

  10. Determinants of Banana Productivity and Technical Efficiency in Uganda

    NARCIS (Netherlands)

    Bagamba, F.; Ruben, R.; Rufino, M.C.

    2007-01-01

    This research report highlights findings from a set of studies undertaken by applied economists on the impact of improved banana cultivars and recommended management practices in the East African highlands. A particular focus of the analysis is genetic transformation of the cooking banana. Genetic t

  11. Highly efficient Agrobacterium-mediated transformation of embryogenic cell suspensions of Musa acuminata cv. Mas (AA) via a liquid co-cultivation system.

    Science.gov (United States)

    Huang, Xia; Huang, Xue-Lin; Xiao, Wang; Zhao, Jie-Tang; Dai, Xue-Mei; Chen, Yun-Feng; Li, Xiao-Ju

    2007-10-01

    A high efficient protocol of Agrobacterium-mediated transformation of Musa acuminata cv. Mas (AA), a major banana variety of the South East Asia region, was developed in this study. Male-flower-derived embryogenic cell suspensions (ECS) were co-cultivated in liquid medium with Agrobacterium strain EHA105 harboring a binary vector pCAMBIA2301 carrying nptII and gusA gene in the T-DNA. Depending upon conditions and duration of co-cultivation in liquid medium, 0-490 transgenic plants per 0.5 ml packed cell volume (PCV) of ECS were obtained. The optimum duration of inoculation was 2 h, and the highest transformation frequency was achieved when infected ECS were co-cultivated in liquid medium first for 12 h at 40 rpm and then for 156 h at 100 rpm on a rotary shaker. Co-cultivation for a shorter duration (72 h) or shaking constantly at 100 rpm at the same duration gave 1.6 and 1.8 folds lower transformation efficiency, respectively. No transgenic plants were obtained in parallel experiments carried on semi-solid media. Histochemical GUS assay and molecular analysis in several tissues of the transgenic plants demonstrated that foreign genes were stably integrated into the banana genome. Compared to semi-solid co-cultivation transformation in other banana species, it is remarkable that liquid co-cultivation was much more efficient for transformation of the Mas cultivar, and was at least 1 month faster for regenerating transgenic plants.

  12. First report of fusarium oxysporum f. sp. cubense tropical race 4 causing panama disease in cavendish bananas in Pakistan and Lebanon

    NARCIS (Netherlands)

    Ordoñez, N.; García-Bastidas, F.; Laghari, H.B.; Akkary, M.Y.; Harfouche, E.N.; Awar, al B.N.; Kema, G.H.J.

    2016-01-01

    Panama disease of banana, caused byFusarium oxysporumf. sp.cubense(Foc), poses a great risk to global banana production. Tropical race 4 (TR4) of Foc, which affects Cavendish bananas as well as many other banana cultivars (Ploetz 2006), was confirmed for the first time outside Southeast Asia in Jord

  13. BABA诱导香蕉果实抗病性与贮藏期活性氧积累的关系%Accumulation of Reactive Oxygen Species Related to Disease Resistance Induced by BABA in Postharvest Banana (Musa AAA. cv. Brazil) Fruit

    Institute of Scientific and Technical Information of China (English)

    谭卫萍; 庞学群; 张昭其; 黄雪梅

    2014-01-01

    果实的抗病性。%[Objective]Effects of β-aminobutyric acid (BABA) on diseases-tolerance of harvested banana (Musa AAA Group cv. Brazil) and the possible mechanism were investigated to provide theoretical basis for the new technology of resistant and preservation.[Method] In this study, diphenylene iodonium (DPI), a NADPH oxidase specific inhibitor, was used to inhibit ROS generation in BABA treated fruits. After 5 g·L-1 BABA treatment or 3.14 mg·L-1DPI followed with 5 g·L-1BABA treatment, banana fruits were inoculated withCollectotrichum musae (2× 105 spores/mL) at 0, 3, 6, 12, 24, 48, and 72 h, respectively, and stored at20±2℃ and RH 85%-95%. The disease spot sizes were determined at 5 d to 16 d. The fruit inoculated withC. musae at 24 h after BABA or DPI treatments and stored at (20±2)℃ and RH85%-95%, and the peel was used for determination of production rate, activities of catalase (CAT) and ascorbate peroxidase (APX), β-1, 3-glucanase(GUN), phenylalanine ammonia lyase(PAL), chitinase (CHI), gene expressions ofMaCAT,MaAPX,MaGLU, MaCHI,MaPAL1andMaNOX during storage.[Result] BABA treatment effectively reduced the disease spot sizes on the peel of inoculated banana at 24 h after BABA treatment and cultured for 5 d, showing that BABA treatment needed proper time to have an effect. The production rate andMaNOX gene expression of BABA treated fruits were evidently higher than those of control during 5-12 d storage. Compared with the control, CAT activity andMaCAT expression were higher on day 5 and 1-5 d, respectively. APX activity andMaAPX expression were higher on 5-8 d and 14 d, and 1-5 d, respectively. CHI activity andMaCHI expression was higher on 5-12 d and 1-5 d, respectively. Both of GUN activity and MaGLU expression were higher on 8-14 d. Both PAL activity andMaPAL1 gene expression were higher on 12-14 d . No significant difference was found in other time points. DPI combined with BABA treatment suppressed the above effects of BABA treatment.[Conclusion]These results strongly suggest

  14. Suscetibilidade de oito cultivares de bananeira à sigatoka-amarela na região de Marília (SP Susceptibility to sigatoka of eight banana cultivars in the region of Marília, State of São Paulo, Brazil

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    Luiz Antonio Junqueira Teixeira

    1996-01-01

    Full Text Available A suscetibilidade de oito cultivares de bananeira à sigatoka-amarela (Mycosphaerella musicola foi avaliada em um experimento de campo, na região de Marília (SP. Três cultivares pertenciam ao Grupo AAA ('Nanicão', 'Nanicão Jangada' e 'Grande Naine' e cinco, ao AAB ('Prata', 'Enxerto', 'Maçã', 'Mysore' e 'Prata Zulu'. O experimento foi implantado em fevereiro de 1992 e, durante os três primeiros anos, não foi feito controle de sigatoka. A suscetibilidade dos materiais foi estimada mediante avaliações em três épocas: março de 93, abril e julho de 94. Na primeira avaliação, foi contado o número de manchas na segunda folha e, nas outras, foram atribuídas notas de acordo com a intensidade dos sintomas. Os cultivares foram agrupados em três classes: altamente suscetível (AS, suscetível (S ou parcialmente resistente (PR. Nenhum dos cultivares pôde ser considerado imune, pois todos apresentaram lesões foliares. Os cultivares mais suscetíveis foram os do Grupo AAA, Subgrupo Cavendish ('Nanicão', 'Grande Naine' e 'Nanicão Jangada' e 'Enxerto' (AAB. 'Mysore' (AAB e 'Prata Zulu' (AAB foram classificados como parcialmente resistentes. 'Prata' (AAB e 'Maçã' (AAB mostraram-se medianamente suscetíveis.Susceptibility of eight banana cultivars to sigatoka caused by Mycosphaerella musicola was assessed in a field experiment in the region of Marília, State of São Paulo. Three cultivars were members of AAA Group ('Nanicão', 'Nanicão Jangada' and 'Grande Naine' and five belonged to AAB Group ('Prata', 'Enxerto', 'Maçã', 'Mysore' and 'Prata Zulu'. The experiment was established in February, 1992, and during the first three years, sigatoka disease was not controlled. Susceptibility was assessed in three occasions: March, 1993, April and July, 1994. In the first, it was counted the number of spots on the second leaf, and in the others, the plants were graded according to the amount of leaf scorch due to sigatoka. The cultivars were

  15. Análises técnica e econômica da cultura da bananeira 'Maçã' (Musa spp. na região noroeste do Estado de São Paulo Technical and economical analysis of 'Apple' banana tree (Musa spp. culture, in the northwest region of São Paulo State

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    Mariley de Castro Almeida da Silva

    2005-04-01

    Full Text Available Este trabalho foi realizado no Escritório de Desenvolvimento Rural - Jales, região noroeste do Estado de São Paulo, com o objetivo de efetuar um levantamento do potencial da cultura da banana 'Maçã' na região, determinando-se indicadores técnicos e econômicos de dois sistemas de produção, os quais utilizam, no plantio, mudas micropropagadas e convencional. O levantamento de dados necessários à realização da pesquisa nos aspectos ligados à elaboração das matrizes de coeficientes técnicos, base para estimativas de custos de produção, foi obtido diretamente junto a produtores da região, e a metodologia de custos foi baseada no custo total de produção. Os resultados econômicos mostraram-se satisfatórios na região para os dois sistemas de produção, mas a receita líquida obtida com a utilização de mudas micropropagadas foi 34% maior que a obtida no sistema convencional.This work has been carried out by the Rural Development Office of Jales, Northwest of São Paulo State, intending to survey the potential of the 'Apple' banana 's cultivation in this region, as well as determine the technical and economical indicators of two production systems, both using micro-propagated and conventional seedlings in the planting. The necessary data survey to accomplish this research in the aspects related to the elaboration of technical coefficient matrixes, a basis for estimating production costs, was obtained among the region producers, and the cost methodology was based in the total cost of production. The economical results turned out to be quite satisfactory in this region for both production systems, however the liquid income obtained from the utilization of micro-propagated seedlings was 34% greater than the one obtained from the conventional system.

  16. In vitro propagation and assessment of the genetic fidelity of Musa acuminata (AAA) cv. Vaibalhla derived from immature male flowers.

    Science.gov (United States)

    Hrahsel, Lalremsiami; Basu, Adreeja; Sahoo, Lingaraj; Thangjam, Robert

    2014-02-01

    An efficient in vitro propagation method has been developed for the first time for Musa acuminata (AAA) cv. Vaibalhla, an economically important banana cultivar of Mizoram, India. Immature male flowers were used as explants. Murashige and Skoog's (MS) medium supplemented with plant growth regulators (PGRs) were used for the regeneration process. Out of different PGR combinations, MS medium supplemented with 2 mg L(-1) 6-benzylaminopurine (BAP) + 0.5 mg L(-1) α-naphthalene acetic acid (NAA) was optimal for production of white bud-like structures (WBLS). On this medium, explants produced the highest number of buds per explant (4.30). The highest percentage (77.77) and number (3.51) of shoot formation from each explants was observed in MS medium supplemented with 2 mg L(-1) kinetin + 0.5 mg L(-1) NAA. While MS medium supplemented with a combination of 2 mg L(-1) BAP + 0.5 mg L(-1) NAA showed the maximum shoot length (14.44 cm). Rooting efficiency of the shoots was highest in the MS basal medium without any PGRs. The plantlets were hardened successfully in the greenhouse with 96% survival rate. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were employed to assess the genetic stability of in vitro regenerated plantlets of M. acuminata (AAA) cv. Vaibalhla. Eight RAPD and 8 ISSR primers were successfully used for the analysis from the 40 RAPD and 30 ISSR primers screened initially. The amplified products were monomorphic across all the regenerated plants and were similar to the mother plant. The present standardised protocol will find application in mass production, conservation and genetic transformation studies of this commercially important banana.

  17. Delayed ripening of banana fruit by salicylic acid.

    Science.gov (United States)

    Srivastava; Dwivedi

    2000-09-08

    Salicylic acid treatment has been found to delay the ripening of banana fruits (Musa acuminata). Fruit softening, pulp:peel ratio, reducing sugar content, invertase and respiration rate have been found to decrease in salicylic acid treated fruits as compared with control ones. The activities of major cell wall degrading enzymes, viz. cellulase, polygalacturonase and xylanase were found to be decreased in presence of salicylic acid. The major enzymatic antioxidants namely, catalase and peroxidase, were also found to be decreased in presence of salicylic acid during banana fruit ripening.

  18. Fungi obtained on various media from soil under banana trees near Logos in Nigeria

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    Aleksandra Ihnatowicz

    2014-08-01

    Full Text Available From the soil samples collected from beneath various banana plant, Musa paradisiaca L., 96 different species of soil fungi were isolated on medium: Ohio-Agar, Littmans-Agar, Martins Rose Bengal-Agar and identified. Four species of keratinophilic fungi were isolated by means of To-Ka-Va trap-hair method.

  19. Adubação nitrogenada e potássica em bananeira 'Pacovan' (musa AAB, subgrupo prata na chapada do Apodi, Estado do Ceará Nitrogen and potassium fertilization for banana 'Pacovan' (musa AAB, prata subgroup at the Apodi plateau in the State of Ceará

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    Olmar Baller Weber

    2006-04-01

    Full Text Available Este trabalho teve por objetivo avaliar o efeito do nitrogênio e do potássio na produção de bananeiras 'Pacovan', sob irrigação, durante três ciclos, e a qualidade dos frutos obtidos no primeiro ciclo de cultivo na chapada do Apodi, no Estado do Ceará, Brasil. A adubação de base consistiu na aplicação de 20 L planta-1 de esterco de curral, 200 g planta-1 de fosfato monoamônico e 100 g planta-1 de FTE-BR12. A partir de 4,5 meses do plantio, mensalmente, aplicou-se 1/12 das doses de N:K2O (180:330; 180:770; 420:330; 420:770; 30:330; 570:770; 180:55; 420:1.045 e 300:550 kg ha-1, utilizando-se como fonte da uréia e do cloreto de potássio. Não houve influência do potássio na produtividade durante os três ciclos de cultivo, sugerindo-se a dose de 55 kg de K2O ha-1 ano-1. Entretanto, a adubação nitrogenada aumentou o número de frutos no cacho durante o segundo ciclo, permitindo estimar a dose ótima (198,3 kg de N ha-1 ano-1. Os teores de sólidos solúveis, açúcares solúveis e acidez titulável total das bananas colhidas durante o primeiro ciclo foram afetados pela adubação nitrogenada e potássica.The objective of this work was to evaluate nitrogen and potassium effects on the production of banana trees 'Pacovan', under irrigation, during three cycles, and the fruit quality during the 1st cycle, at the Apodi plateau in the State of Ceará, Brazil. The basic fertilization consisted of the application of 20 L plant-1 of cattle manure, 200 g plant-1 of mono-ammonium phosphate and 100 g of FTE-BR12 plant-1. Four and a half months aster planting, were applied, monthly, 1/12 of the doses of N:K2O (180:330; 180:770; 420:330; 420:770; 30:330; 570:770; 180:55; 420:1045 e 300:550 kg ha-1, using as source, urea and potassium chloride. The crop productivity was not affected by potassium during three yield cycles, suggesting the dose of 55 kg ha-1 year-1 of K2O. However, the nitrogen fertilization increased the number of fruits on

  20. RESISTANT STARCH AND BIOACTIVE CONTENTS OF UNRIPE BANANA FLOUR AS INFLUENCED BY HARVESTING PERIODS AND ITS APPLICATION

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    Anuchita Moongngarm

    2014-01-01

    Full Text Available Recently, various innovative products from unripe banana flour have been reported as it is high in resistant starch and other important compounds. The harvesting period of the unripe banana fruit is one of the key factors affecting the quality of the unripe banana flour in terms of resistant starch and bioactive compound content. The study aimed to investigate the effect of the harvesting stages of unripe banana fruit on Resistant Starch (RS content, carotenoid content, antioxidant activity and the application of unripe banana flour to prepare high RS rice noodle. Four different harvesting stages of banana fruits of Musa sapientum Linn including 75, 90, 105 and 120 days after bloom, were processed for banana flours. The results indicated that the maturation stages affected RS, some bioactive contents, antioxidant activities. The highest RS content (48.88% of banana flour was obtained from the 105 day banana fruits. The total phenolic and carotenoid contents were high in the banana flours harvested between 75 and 105 days. The unripe banana flour could be substituted for rice flour as high as 80% and contained RS content as high as 18.64% whereas the commercial rice noodle had 4.21% of RS content. Therefore, the preparation of unripe banana flour from banana fruit harvested at 105 days and applying it in the preparation of functional food is promising.

  1. Avaliação de clones de bananeira do subgrupo Cavendish (Musa acuminata, AAA em Cruz das Almas-BA Evaluation of banana Cavendish clones in Cruz das Almas City, Bahia State, Brazil

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    Sebastião de Oliveira e Silva

    2004-12-01

    Full Text Available Foram avaliados em Cruz das Almas-BA, em dois ciclos, 69 clones Cavendish coletados em São Paulo, Minas Gerais, Bahia e Santa Catarina, sendo 49 do tipo Nanicão e 20 do tipo Grande Naine. Os clones foram estabelecidos em área experimental da Embrapa Mandioca e Fruticultura utilizando-se um delineamento em blocos ao acaso com cinco repetições e cinco plantas por parcela. Foram utilizados a irrigação por microaspersão, o espaçamento de 3,0 m x 2,0 m e um sistema de condução com três plantas/touceira. Os requerimentos agronômicos foram aplicados conforme preconizados para o cultivo. Na avaliação dos clones, foram consideradas as seguintes variáveis: altura da planta em centímetros (AP; número de dias do plantio à colheita (ND; peso do cacho em quilogramas (PC; número de frutos por cacho (NF; e comprimento do fruto em centímetros (CF. Pelos resultados obtidos, verificou-se uma variabilidade média em todas as variáveis avaliadas. Por apresentarem caracteres superiores, foram selecionados cinco clones de Grande Naine (G.N. Taperão, G.N.Rossete, G.N. Willians, G.N. Magário, G.N. SC-074 e quatro de Nanicão (N. IAC Abóbada Verde, N. Rossete, N. SC-0008 e N. SC-063.Sixty-nine clones of Cavendish type bananas (49 Nanicão and 20 Grande Naine collected in the States of São Paulo, Minas Gerais, Bahia and Santa Catarina were evaluated. The clones were established in an experimental area at the Cassava and Fruit Crops Research Center - EMBRAPA, using micro-aspersion irrigation, in plants spaced at 3.0 m x 2.0 m, and conducted with three plants/bushes. Random blocks were used with five replications and five plants per plot. All cultural requirements for the crop were carried out. For the clone evaluations, the following variables were considered: plant height in centimeters (PH; number of days from planting to yield (ND; weight of bunch in kilograms (WB; number of fruits per bunch (FB and length of fruit in centimeters (LF. The

  2. Identification of genes differentially expressed during ripening of banana.

    Science.gov (United States)

    Manrique-Trujillo, Sandra Mabel; Ramírez-López, Ana Cecilia; Ibarra-Laclette, Enrique; Gómez-Lim, Miguel Angel

    2007-08-01

    The banana (Musa acuminata, subgroup Cavendish 'Grand Nain') is a climacteric fruit of economic importance. A better understanding of the banana ripening process is needed to improve fruit quality and to extend shelf life. Eighty-four up-regulated unigenes were identified by differential screening of a banana fruit cDNA subtraction library at a late ripening stage. The ripening stages in this study were defined according to the peel color index (PCI). Unigene sequences were analyzed with different databases to assign a putative identification. The expression patterns of 36 transcripts confirmed as positive by differential screening were analyzed comparing the PCI 1, PCI 5 and PCI 7 ripening stages. Expression profiles were obtained for unigenes annotated as orcinol O-methyltransferase, putative alcohol dehydrogenase, ubiquitin-protein ligase, chorismate mutase and two unigenes with non-significant matches with any reported sequence. Similar expression profiles were observed in banana pulp and peel. Our results show differential expression of a group of genes involved in processes associated with fruit ripening, such as stress, detoxification, cytoskeleton and biosynthesis of volatile compounds. Some of the identified genes had not been characterized in banana fruit. Besides providing an overview of gene expression programs and metabolic pathways at late stages of banana fruit ripening, this study contributes to increasing the information available on banana fruit ESTs.

  3. Cultivable bacteria populations associated with leaves of banana and plantain plants and their antagonistic activity against Mycosphaerella fijiensis.

    Science.gov (United States)

    Ceballos, Isabel; Mosquera, Sandra; Angulo, Mónica; Mira, John J; Argel, Luz Edith; Uribe-Velez, Daniel; Romero-Tabarez, Magally; Orduz-Peralta, Sergio; Villegas, Valeska

    2012-10-01

    Mycosphaerella fijiensis is the etiological agent of Black Sigatoka, a fungal disease that affects production of banana and plantain crops in tropical regions. The sizes of cultivable epiphytic and endophytic bacterial populations, aerobic endospore forming bacteria (AEFB), and antagonist bacteria against M. fijiensis isolated from three Musa spp. cultivars from Urabá (Colombia) were studied, in order to find a suitable screening strategy to isolate antagonistic bacteria. Most of the variability found in the epiphytic and endophytic bacterial community sizes among fruit trees was explained by the cultivar differences. We found population sizes ranging from 1.25 × 10(3) to 9.64 × 10(5) CFU/g of fresh leaf and found that 44 % of total cultivable bacteria belong to the AEFB group. We isolated 648 AEFB from three different cultivars and assessed their antagonistic activity against M. fijiensis using the cell-free supernatant obtained from bacterial liquid cultures in three different in vitro assays. Five percent of those bacteria showed higher percent inhibition than the positive control Bacillus subtilis UA321 has (percent inhibition = 84 ± 5) in the screening phase. Therefore, they were selected as antagonistic bacteria against the pathogen. The strains with the highest percentage of antagonism were found in older leaves for the three cultivars, given support to recommend this group of leaves for future samplings. Some of these isolated bacteria affected the mycelium and ascospores morphology of the fungus. They also presented in vitro characteristics related to a successful colonization of the phylloplane such as indolic compounds, surfactant production, and biofilm formation, which makes them possible, potential candidates as biological control agents.

  4. Traditional and Medicinal Uses of Banana

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    K. P. Sampath Kumar

    2012-09-01

    Full Text Available Banana is the common name for herbaceous plants of the genus Musa and for the fruit they produce. It is one of the oldest cultivated plants. All parts of the banana plant have medicinal applications: the flowers in bronchitis and dysentery and on ulcers; cooked flowers are given to diabetics; the astringent plant sap in cases of hysteria, epilepsy, leprosy, fevers, hemorrhages, acute dysentery and diarrhea, and it is applied on hemorrhoids, insect and other stings and bites; young leaves are placed as poultices on burns and other skin afflictions; the astringent ashes of the unripe peel and of the leaves are taken in dysentery and diarrhea and used for treating malignant ulcers; the roots are administered in digestive disorders, dysentery and other ailments; banana seed mucilage is given in cases of diarrhea in India. Antifungal and antibiotic principles are found in the peel and pulp of fully ripe bananas. The antibiotic acts against Mycobacteria. A fungicide in the peel and pulp of green fruits is active against a fungus disease of tomato plants. Norepinephrine, dopamine, and serotonin are also present in the ripe peel and pulp. The first two elevate blood pressure; serotonin inhibits gastric secretion and stimulates the smooth muscle of the intestines.

  5. EFFECT OF NUMBER OF FUNCTIONAL LEAVES AT FLOWERING ON YIELD OF BANANA GRAND NAINE (Musa AAA Simmonds EFECTO DEL NÚMERO DE HOJAS FUNCIONALES A LA FLORACIÓN SOBRE LA PRODUCCIÓN DE BANANO GRAN ENANO (Musa AAA Simmonds

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    Carolina Rodríguez González

    2012-06-01

    Full Text Available Abstract. The effect of the selective removal of leaves at flowering on yield, growth, maturity and quality of Grand Nain banana fruits was evaluated. The present study was conducted in three regions of Urabá (Colombia with precipitation low (experiment 1, medium (experiment 2 and high (experiment 3. The experimental design used randomized complete blocks, four treatments (6, 8, 10 and 12 leaves from the start of flowering and three replications. Every 15 days, the thickness (cm and length (cm of central fruit from the second hand were measured and two fruits were taken to record fresh weight and dry weight. At harvest, leaf number, bunch weight (kg, and the thickness (cm and length (cm of central fruit from the second hand were measured. During fruit ripening (8 and 15 days, the contents of starch and sugars in the pulp were determined. The thickness and length of the fruit, bunch weight and the box-stem ratio were not affected (PResumen. Se evaluó el efecto de la eliminación selectiva de hojas a la floración sobre la producción, crecimiento, maduración y calidad de los frutos de banano Gran Enano. El estudio se realizó en tres zonas de Urabá (Colombia, con precipitación baja (experimento 1, media (experimento 2 y alta (experimento 3. Se usó un diseño de bloques completamente al azar, cuatro tratamientos (6, 8, 10 y 12 hojas desde el inicio de floración y tres repeticiones. Cada 15 días se midieron el grosor (cm y la longitud (cm del fruto central de la segunda mano y se tomaron dos frutos más para registrar el peso fresco y el peso seco. A la cosecha se midieron el número de hojas, el peso del racimo (kg, el grosor (cm y la longitud (cm del fruto central de la segunda mano. Durante la maduración de los frutos (8 y 15 días se determinaron los contenidos de almidón y azúcares en la pulpa. El grosor y la longitud del fruto, peso del racimo y la cantidad de cajas a partir de un racimo (ratio, no fueron afectados (P<0,05 por la

  6. Caracterização da farinha de banana verde Green banana flour characterization

    OpenAIRE

    Antonia de Maria Borges; Joelma Pereira; Eliseu Marlônio Pereira de Lucena

    2009-01-01

    O presente trabalho objetivou a obtenção, a caracterização físico-química e o controle microbiológico durante o processamento da farinha de banana (Musa spp.) verde, cv. Prata, visando o seu aproveitamento na panificação, produtos dietéticos e alimentos infantis. Para obtenção da farinha, os frutos foram cortados, imersos em metabissulfito de sódio, desidratados e triturados, sendo em seguida, feitas as seguintes determinações: umidade; extrato etéreo; proteína bruta; fibra bruta; cinzas; fra...

  7. Effect of physiological harvest stages on the composition of bioactive compounds in Cavendish bananas.

    Science.gov (United States)

    Bruno Bonnet, Christelle; Hubert, Olivier; Mbeguie-A-Mbeguie, Didier; Pallet, Dominique; Hiol, Abel; Reynes, Max; Poucheret, Patrick

    2013-04-01

    The combined influence of maturation, ripening, and climate on the profile of bioactive compounds was studied in banana (Musa acuminata, AAA, Cavendish, cv. Grande Naine). Their bioactive compounds were determined by the Folin-Ciocalteu assay and high-performance thin layer chromatographic (HPTLC) method. The polyphenol content of bananas harvested after 400 degree days remained unchanged during ripening, while bananas harvested after 600 and 900 degree days exhibited a significant polyphenol increase. Although dopamine was the polyphenol with the highest concentration in banana peels during the green developmental stage and ripening, its kinetics differed from the total polyphenol profile. Our results showed that this matrix of choice (maturation, ripening, and climate) may allow selection of the banana (M. acuminata, AAA, Cavendish, cv. Grande Naine) status that will produce optimal concentrations of identified compounds with human health relevance.

  8. Effect of physiological harvest stages on the composition of bioactive compounds in Cavendish bananas

    Institute of Scientific and Technical Information of China (English)

    Christelle BRUNO BONNET; Olivier HUBERT; Didier MBEGUIE-A-MBEGUIE; Dominique PALLET; Abel HIOL; Max REYNES; Patrick POUCHERET

    2013-01-01

    The combined influence of maturation,ripening,and climate on the profile of bioactive compounds was studied in banana (Musa acuminata,AAA,Cavendish,cv.Grande Naine).Their bioactive compounds were determined by the Folin-Ciocalteu assay and high-performance thin layer chromatographic (HPTLC) method.The polyphenol content of bananas harvested after 400 degree days remained unchanged during ripening,while bananas harvested after 600 and 900 degree days exhibited a significant polyphenol increase.Although dopamine was the polyphenol with the highest concentration in banana peels during the green developmental stage and ripening,its kinetics differed from the total polyphenol profile.Our results showed that this matrix of choice (maturation,ripening,and climate) may allow selection of the banana (M.acuminata,AAA,Cavendish,cv.Grande Naine) status that will produce optimal concentrations of identified compounds with human health relevance.

  9. Comportamento de variedades e híbridos de bananeira (Musa spp., em dois ciclos de produção no sudoeste da Bahia Behavior of banana varieties and hybrids (Musa spp., in two production cycle in the southwest of Bahia State

    Directory of Open Access Journals (Sweden)

    Sérgio Luiz Rodrigues Donato

    2006-04-01

    characterize and evaluate the behavior of 13 banana genotypes (varieties and hybrids AAA, AAAA, AAB and AAAB genomic groups, in two production cycles in southwest Bahia, using relevant phenotypic descriptors for the identification and selection of superior individuals. The Prata Anã and Pacovan (AAB, Grande Naine and Nanicão (AAA varieties and the hybrids PA42-44, Pacovan Ken, Japira, Preciosa and ST12-31 (AAAB and Ambrosia, Calipso, Bucaneiro and FHIA02 (AAAA, selected at Embrapa Cassava and Fruit Crops, were evaluated. The experimental design was in random blocks, with 13 treatments, five repetitions, 20 plants per plot, six useful and 3.00 m x 2.00 m spacing. The following characteristics were considered: Plant height, pseudostem perimeter, number of live leaves during flowering and harvest, number of days from planting to flowering and until harvest, bunch and hand weight, number of fruits, weight, length and diameter of fruit. Grande Naine, and Nanicão stood out for the characteristics of bunch and hand weight, number of fruits, fruit weight and length; the FHIA-02 hybrid presented greater fruit diameter in both production cycles and the earliest flowering and harvest in the second cycle; Calipso was the latest for harvest; ST12-31 was the tallest in height; the Prata Anã variety stood out for number of live leaves during flowering and harvest; the Prata type hybrids, PA42-44, Pacovan Ken, Preciosa and Japira, stood out for the parameters related to fruit quality when compared to its genitors Prata Anã and Pacovan, respectively; there was an increment of the parameters that reflected vegetative growth and yield from the first to the second production cycle for all genotypes evaluated.

  10. Characterization of a linear DNA plasmid from the filamentous fungal plant pathogen Glomerella musae [Anamorph: Colletotrichum musae (Berk. and Curt.) arx.

    Science.gov (United States)

    Freeman, S.; Redman, R.S.; Grantham, G.; Rodriguez, R.J.

    1997-01-01

    A 7.4-kilobase (kb) DNA plasmid was isolated from Glomerella musae isolate 927 and designated pGML1. Exonuclease treatments indicated that pGML1 was a linear plasmid with blocked 5' termini. Cell-fractionation experiments combined with sequence-specific PCR amplification revealed that pGML1 resided in mitochondria. The pGML1 plasmid hybridized to cesium chloride-fractionated nuclear DNA but not to A + T-rich mitochondrial DNA. An internal 7.0-kb section of pGML1 was cloned and did not hybridize with either nuclear or mitochondrial DNA from G. musae. Sequence analysis revealed identical terminal inverted repeats (TIR) of 520 bp at the ends of the cloned 7.0-kb section of pGML1. The occurrence of pGML1 did not correspond with the pathogenicity of G. musae on banana fruit. Four additional isolates of G. musae possessed extrachromosomal DNA fragments similar in size and sequence to pGML1.

  11. Pengaruh Kombinasi Pemupukan Terhadap Pertumbuhan Pisang Kepok Kuning (Musa acuminata × M. balbisiana Pada Lahan Kering di Banyumas, Jawa Tengah

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    Saktiyono Sigit Tri Pamungkas

    2015-06-01

    Full Text Available Kepok Kuning banana (Musa acuminate × M.balbisiana is a banana which has a good adaptability tothe water shortage conditions. The banana has cultivatedin watershed of Tajum river, Banyumas. The place haspotential to be centre of Yellow Kepok banana productionbut the farmers occasionally applied fertilizer unbalancedwhich effect to productivity and quality of banana. It isnecessary to examine proportion of fertilizers to determinethe effective and effi cient fertilizer composition whichincreased productivity and quality of banana.The experiment was conducted on Gentawangi dryland farm, Jatilawang, Banyumas on January-June 2009. Theexperiment was laid out in randomized block design with12 replications and involving single factor. It consistedof 5 treatments The Banana was planted in pattern 3 m x3 m. Results showed that banana required both of organicfertilizers and inorganic in vegetative stage. The treatmentof Urea 150 g + 100 g + SP36 compost 10 kg/tree/applicationshowed the effi cient compared to other, followed bytreatment of ZA 150 g + SP36 KCl 150 g + 50 g/tree/application. Combination of Urea and ZA without organicfertilizer indicated uneffi cient compared to others

  12. Progress of Methodology of Genetic Transformation of Banana%香蕉遗传转化方法研究进展

    Institute of Scientific and Technical Information of China (English)

    黄永红; 易干军; 周碧容; 曾继吾; 吴元立

    2006-01-01

    Banana is an important staple food and source of carbohydrates in developing counties of tropical and subtropical. But many pests and diseases have significantly affected banana cultivation. Most cultivars of banana are triploid, long life cycle, and sterility, which resulted in difficulty for its reproduction and breeding. In order to conquer limitations of conventional breeding and to improve cultivars qualities, transgenic approaches have been introduced. The development of transgenic Musa plants has been achieved using electroporation, microprojectile bombardment procedure, and A grobacterium-mediated transformation etc. The successful application of A grobacterium- mediated transformation protocol is a great breakthrough in the development of banana cultivars improvement. The explants of the genetic transformation of Musa have developed into protoplast, embryogenic cell suspensions, meristematic tissues and thin cell layer. Presently, with the advancement of biology, some more efficient genetic transformation methods including integrated bombardment and Agrobacterium transformation, centrifugation-assisted Agrobacterium-mediated transformation (CAAT) protocol, and vacuum infiltration technique have been developed and made great progress, which will accelerate the progress of banana breeding.%香蕉是热带亚热带发展中国家重要的粮食作物和碳水化合物来源.但近年来,香蕉生产受到严重的病虫危害.大多数香蕉栽培品种是三倍体,生长周期长,而且不孕.由于没有种子,给繁殖和育种带来一定的困难.遗传转化技术的发展为香蕉品种的改良提供了一种有效的手段.香蕉的遗传转化方法有电激法、基因枪法、农杆菌介导法等.农杆菌介导法的应用是香蕉品种改良的一个重大突破.香蕉遗传转化的外植体也发展到多种,有原生质体,胚性细胞悬浮系,分生组织,以及横切薄片等.近几年,随着分子生物学的发展,出现了转化效

  13. Inibição do amadurecimento da banana-'Prata-Anã' com a aplicação do 1-metilciclopropeno Inhibition of the ripening of banana 'Prata-Anã' by 1-methylcyclopropene

    Directory of Open Access Journals (Sweden)

    NEIDE BOTREL

    2002-04-01

    Full Text Available A banana é uma das frutas mais consumidas no mundo, cultivada na maioria dos países tropicais. As práticas de pós-colheita, muitas vezes, não são suficientes para garantir uma boa qualidade da fruta quando esta é comercializada em mercados mais distantes. O 1-metilciclopropeno (1-MCP é um produto bloqueador da ação do etileno e tem sido utilizado com sucesso em flores, hortaliças e frutos. O objetivo deste trabalho foi retardar o amadurecimento da banana-'Prata' e aumentar a sua vida útil, testando diferentes níveis do 1- MCP . Os frutos da cultivar 'Prata-Anã', provenientes de Janaúba, Norte de Minas Gerais, Brasil, foram colhidos no estádio de maturação 1, que corresponde ao estádio verde, classificados como "extra", selecionados e separados em parcelas de 10 frutos, com 3 repetições para cada tratamento. Foram utilizados 4 níveis do produto 0; 10; 30 e 90 ppb. O 1-MCP foi utilizado na formulação pó, na concentração de 0,14% de ingrediente ativo. Os frutos foram armazenados em temperatura ambiente (24ºC e 78,5 % UR e avaliados aos 0; 4; 8 e 12 dias após a aplicação do produto. As avaliações foram: cor, despencamento de dedos no buquê, firmeza, sólidos solúveis, acidez titulável e pH. Com 30 ppb, o amadurecimento foi retardado por 4 dias e, com 90ppb, por 8 dias. Essa técnica apresenta-se como uma alternativa para prolongar a vida útil dos frutos dessa cultivar.Banana, Musa spp., is one of the most consumed fruits around the world, and it is cultivated in most of tropical countries. Often postharvest practices are not good enough to guarantee the quality of the fruits mainly if they are to be commercialized at distant markets. The 1-methylcyclopropene (1-MCP is known as a blocking of ethylene action and it has been successfully used in flowers, vegetables and fruits. The aim of this work was to delay the ripening of banana-'Prata' increasing its shelf life. Fruits of Banana -'Prata anã' were used from

  14. Protocol for simultaneous isolation of three important banana allergens.

    Science.gov (United States)

    Nikolic, Jasna; Mrkic, Ivan; Grozdanovic, Milica; Popovic, Milica; Petersen, Arnd; Jappe, Uta; Gavrovic-Jankulovic, Marija

    2014-07-01

    Banana fruit (Musa acuminata) has become an important food allergen source in recent years. So far, 5 IgE reactive banana proteins have been identified, and the major allergens are: Mus a 2 (a class I chitinase, 31kDa), Mus a 4 (thaumatin-like protein, 21kDa), and Mus a 5 (β-1,3-glucanase, 33kDa). Due to variations in allergen expression levels, diagnostic reagents for food allergy can be improved by using individual allergen components instead of banana allergen extracts. The purpose of this study was to optimize the purification protocol of the three major allergens present in banana fruit: Mus a 2, Mus a 4 and Mus a 5. By employing a three-step purification protocol (a combination of anion-exchange, cation-exchange and reversed-phase chromatography) three important banana allergens were obtained in sufficient yield and high purity. Characterization of the purified proteins was performed by both biochemical (2-D PAGE, mass fingerprint and N-terminal sequencing) and immunochemical (immunoblot) methods. IgE reactivity to the purified allergens was tested by employing sera of five allergic patients. The purified allergens displayed higher sensitivity in IgE detection than the routinely used extracts. The three purified allergens are good candidates for reagents in component-based diagnosis of banana allergy.

  15. Residual effects of low oxygen storage of mature green fruit on ripening processes and ester biosynthesis during ripening in bananas

    Science.gov (United States)

    Mature green banana (Musa sapientum L. cv. Cavendish) fruit were stored in 0.5%, 2 %, or 21% O2 for 7 days at 20 °C before ripening was initiated by ethylene. Residual effects of low O2 storage in mature green fruit on ripening and ester biosynthesis in fruit were investigated during ripening period...

  16. Multiple gene genealogies and phenotypic characters differentiate several novel species of Mycosphaerella and related anamorphs on banana

    NARCIS (Netherlands)

    Arzanlou, M.; Groenewald, J.Z.; Fullerton, R.A.; Abeln, E.C.A.; Carlier, J.; Zapater, M.-F.; Buddenhagen, I.W.; Viljoen, A.; Crous, P.W.

    2008-01-01

    Three species of Mycosphaerella, namely M. eumusae, M. fijiensis, and M. musicola are involved in the Sigatoka disease complex of bananas. Besides these three primary pathogens, several additional species of Mycosphaerella or their anamorphs have been described from Musa. However, very little is kno

  17. Carotenoid-rich bananas: a potential food source for alleviating vitamin A deficiency.

    Science.gov (United States)

    Englberger, Lois; Darnton-Hill, Ian; Coyne, Terry; Fitzgerald, Maureen H; Marks, Geoffrey C

    2003-12-01

    This review article points out that bananas are an important food for many people in the world. Thus, banana cultivars rich in provitamin A carotenoids may offer a potential food source for alleviating vitamin A deficiency, particularly in developing countries. Many factors are associated with the presently known food sources of vitamin A that limit their effectiveness in improving vitamin A status. Acceptable carotenoid-rich banana cultivars have been identified in Micronesia, and some carotenoid-rich bananas have been identified elsewhere. Bananas are an ideal food for young children and families for many regions of the world, because of their sweetness, texture, portion size, familiarity, availability, convenience, versatility, and cost. Foods containing high levels of carotenoids have been shown to protect against chronic disease, including certain cancers, cardiovascular disease, and diabetes. Because the coloration of the edible flesh of the banana appears to be a good indicator of likely carotenoid content, it may be possible to develop a simple method for selecting carotenoid-rich banana cultivars in the community. Research is needed on the identification of carotenoid-rich cultivars, targeting those areas of the world where bananas are a major staple food; investigating factors affecting production, consumption, and acceptability; and determining the impact that carotenoid-rich bananas may have on improving vitamin A status. Based on these results, interventions should be undertaken for initiating or increasing homestead and commercial production.

  18. Uso da giberelina GA3 na seleção do porte de bananeira das cultivares prata e prata-anã Use of gibberellin GA3 in the selection of banana height of prata and dwarf prata cultivars

    Directory of Open Access Journals (Sweden)

    Joana Angélica Bonfim Silva de Carvalho

    2005-12-01

    Full Text Available Este trabalho teve como objetivo desenvolver metodologia de seleção do porte em bananeira mediante o emprego de giberelina. Foi desenvolvido em condições controladas de casa de vegetação da Embrapa Mandioca e Fruticultura Tropical, localizada em Cruz das Almas - BA (12° 48' 38" de latitude sul e 39° 06'26" de longitude oeste de Greenwich, no período de setembro de 2002 a janeiro de 2003. Para a comparação de diferentes portes de plantas das cultivares Prata-Anã e Prata-Gigante, foram testadas diferentes doses de ácido giberélico (0; 3,0; 14,0; 29,0; 59,0 e 145 µmol L-1, avaliando-se aos 30 e 60 dias após o plantio, altura da planta e altura da primeira folha. Avaliaram-se também o diâmetro do caule, massas fresca e seca da parte aérea e da raiz, e altura da segunda folha, aos 60 dias após o plantio. A concentração que provocou o maior efeito nos caracteres considerados foi de 84 µmol L-1, sendo que, na variável altura da planta, aos 60 dias foi a de 90,26 µmol L-1. Para todas as variáveis estudadas, observou-se um ponto de máximo em torno da dose de 90 µmol L-1 de giberelina. A concentração de 94,13 µmol L-1 de ácido giberélico (GA3 foi a mais eficiente na identificação precoce do porte de genótipos de Prata-Anã e Prata-Gigante. O momento adequado para efetuar a separação dos genótipos de diferentes portes é aos 60 dias após o plantio, e a variável que deve ser observada no instante da seleção, é a altura da segunda folha.This work aimed to develop a methodology for the selection of plants with different heights using gibberellin. The study was carried out under greenhouse conditions at Embrapa Cassava and Tropical Fruits, located at Cruz das Almas - BA (12º 48' 38" South latitude and 39º 06' 26" longitude West, during the period of September 2002 through January 2003. For the comparison of different heights of Prata Dwarf and Prata Gigante cultivars, different concentrations of gibberellic acid (0

  19. Fungal pathogens associated with banana fruit in Sri Lanka, and their treatment with essential oils.

    Science.gov (United States)

    Anthony, Sulali; Abeywickrama, Krishanthi; Dayananda, Ranjith; Wijeratnam, Shanthi Wilson; Arambewela, Luxshmi

    2004-01-01

    The crown rot pathogens isolated from banana samples collected from 12 localities in Sri Lanka were Lasiodiplodia theobromae, Fusarium proliferatum and Colletotrichum musae. Fungal pathogens isolated were able to cause crown rot disease alone or in combination. Disease severity was higher when combinations of virulent pathogens were used. Cymbopogon nardus and Ocimum basilicum oils displayed fungicidal activity against C. musae and F. proliferatum between 0.2-0.6% (v/v) in a Poisoned food bioassay. Slightly lower concentrations of the test oils were needed for similar activity during liquid bioassays. The combination of Cymbopogon nardus and O. basilicum oils demonstrated synergistic action during both in-vivo bioassays.

  20. Fruit physical characteristics, proximate, mineral and starch characterization of FHIA 19 and FHIA 20 plantain and FHIA 03 cooking banana hybrids.

    Science.gov (United States)

    Annor, George Amponsah; Asamoah-Bonti, Prudence; Sakyi-Dawson, Esther

    2016-01-01

    Cooking banana and plantain (Musa spp. AAB and ABB groups), have over the years been affected by pest and diseases, resulting in various organizations developing disease resistant hybrids with superior agronomic potential. The characteristics of these improved varieties needs to be studied to ascertain their suitability for use in various food systems. This study aimed at evaluating the physical characteristics, proximate and minerals composition, and characterizing the starch of plantain and a cooking banana hybrid release by Fundación Hondureña de Investigación Agrícola (FHIA), and comparing them to a local landrace in Ghana. FHIA 19 and FHIA 20 plantain, Apentu pa (a local landrace) and FHIA 03 cooking banana hybrid were used for the study. Their physical characteristics, proximate and mineral composition were determined at the proximal, midsection and distal hand positions. Starch granules and cells were then examined under light microscope. Ranges obtained for protein content for FHIA 20, FHIA 03 and FHIA 19 were 3.01-3.40, 2.66-2.91 and 2.81-2.91 %. Potassium was found to be the most abundant mineral in all the cultivars. The highest mean value of 982.5-1013.76 mg/100 g was obtained for FHIA 19. There were significant differences (p starch granule size was found in FHIA 19 hybrid. FHIA 03 was also composed predominantly of two types: longitudinal and rounded granules with each type grouped together. The new plantain hybrids compared very well with the local landrace hence making them suitable to be incorporated into local food systems.

  1. Differential characteristics in the chemical composition of bananas from Tenerife (Canary Islands) and Ecuador.

    Science.gov (United States)

    Forster, Markus Paul; Rodríguez Rodríguez, Elena; Díaz Romero, Carlos

    2002-12-18

    The contents of moisture, protein, ash, ascorbic acid, glucose, fructose, total sugars, and total and insoluble fiber were determined in cultivars of bananas (Gran Enana and Pequeña Enana) harvested in Tenerife and in bananas (Gran Enana) from Ecuador. The chemical compositions in the bananas from Tenerife and from Ecuador were clearly different. The cultivar did not influence the chemical composition, except for insoluble fiber content. Variations of the chemical composition were observed in the bananas from Tenerife according to cultivation method (greenhouse and outdoors), farming style (conventional and organic), and region of production (north and south). A highly significant (r = 0.995) correlation between glucose and fructose was observed. Correlations of ash and protein contents tend to separate the banana samples according to origin. A higher content of protein, ash, and ascorbic acid was observed as the length of the banana decreased. Applying factor analysis, the bananas from Ecuador were well separated from the bananas produced in Tenerife. An almost total differentiation (91.7%) between bananas from Tenerife and bananas from Ecuador was obtained by selecting protein, ash, and ascorbic acid content and applying stepwise discriminant analysis. By selecting the bananas Pequeña Enana and using discriminant analysis, a clear separation of the samples according to the region of production and farming style was observed.

  2. Crescimento de bananeiras sob diferentes níveis de salinidade da água de irrigação Growth in banana cultivars under different salinity levels of irrigation water

    Directory of Open Access Journals (Sweden)

    Gilcimar Alves do Carmo

    2003-12-01

    Full Text Available Avaliaram-se os efeitos do uso de diferentes níveis de salinidade da água de irrigação (S1 = 0,55 dS/m, S2 = 1,70 dS/m, S3 = 2,85 dS/m e S1 = 4,00 dS/m no crescimento vegetativo de bananeiras Pacovan (AAB e Marmelo (ABB, utilizando-se um solo Argissolo Crômico, textura franco-argilo-arenosa e adotando-se um delineamento experimental em blocos completos casualizados, com parcelas subdivididas e quatro repetições. As parcelas experimentais foram compostas de uma fileira com 10 plantas, sendo cinco de cada cultivar, com as três plantas centrais de cada sub-parcela consideradas úteis. As irrigações foram feitas diariamente ou a cada dois dias de modo a proporcionar uma fração de lixiviação ao redor de 0,15%, colocando-se volumes aproximados de água para manter o solo próximo à capacidade de campo. Os resultados obtidos aos 110, 160, 220, 300 e 360 dias após o plantio mostraram que o incremento nos níveis de salinidade diminuiu significativamente a altura da planta, o número de folhas e a área foliar com o aumento do nível de salinidade da água até no máximo 240 dias do desenvolvimento, havendo uma equiparação depois do período chuvoso.The effects of different salinity levels of irrigation water (S1 = 0.55 dS/m, S2 = 1.70 dS/m, S3 = 2.85 dS/m e S4 = 4.00 dS/m on vegetative growth in bananas Pacovan (AAB and Marmelo (ABB were evaluated. The experiment was carried out on a sandy clay loam Chromic Argisol in a split-plot scheme arranged in a randomized block design with four replications. The whole plots were composed of a row with 10-plants, five plants of each cultivar (subplot, and the data were collected from the three central ones. The irrigations were applied either dayly or every each two-days period in order to maintain a lixiviation fraction of 0.15, using approximate water sheets to keep the soil at field capacity. The data obtained at 110, 160, 220, 300, and 360 days after planting indicated that the water

  3. Artificial neural network modelling of the antioxidant activity and phenolic compounds of bananas submitted to different drying treatments.

    Science.gov (United States)

    Guiné, Raquel P F; Barroca, Maria João; Gonçalves, Fernando J; Alves, Mariana; Oliveira, Solange; Mendes, Mateus

    2015-02-01

    Bananas (cv. Musa nana and Musa cavendishii) fresh and dried by hot air at 50 and 70°C and lyophilisation were analysed for phenolic contents and antioxidant activity. All samples were subject to six extractions (three with methanol followed by three with acetone/water solution). The experimental data served to train a neural network adequate to describe the experimental observations for both output variables studied: total phenols and antioxidant activity. The results show that both bananas are similar and air drying decreased total phenols and antioxidant activity for both temperatures, whereas lyophilisation decreased the phenolic content in a lesser extent. Neural network experiments showed that antioxidant activity and phenolic compounds can be predicted accurately from the input variables: banana variety, dryness state and type and order of extract. Drying state and extract order were found to have larger impact in the values of antioxidant activity and phenolic compounds.

  4. Protection of ultrastructure in chilling-stressed banana leaves by salicylic acid

    Institute of Scientific and Technical Information of China (English)

    KANG Guo-zhang; WANG Zheng-xun; XIA Kuai-fei; SUN Gu-chou

    2007-01-01

    Objective: Chilling tolerance of salicylic acid (SA) in banana seedlings (Musa acuminata cv., Williams 8818) was investigated by changes in ultrastructure in this study. Methods: Light and electron microscope observation. Results: Pretreatment with 0.5 mmol/L SA under normal growth conditions (30/22 ℃) by foliar spray and root irrigation resulted in many changes in ultrastructure of banana cells, such as cells separation from palisade parenchymas, the appearance of crevices in cell walls, the swelling of grana and stromal thylakoids, and a reduction in the number of starch granules. These results implied that SA treatment at 30/22 ℃ could be a type of stress. During 3 d of exposure to 7 ℃ chilling stress under low light, however, cell ultrastructure of SA-pretreated banana seedlings showed less deterioration than those of control seedlings (distilled water-pretreated). Conclusion:SA could provide some protection for cell structure of chilling-stressed banana seedling.

  5. Chemical control of the red palm mite, Raoiella indica (Acari: Tenuipalpidae) in banana and coconut.

    Science.gov (United States)

    Rodrigues, Jose Carlos Verle; Peña, J E

    2012-08-01

    The red palm mite (RPM), Raoiella indica Hirst, is a predominant pest of coconuts, date palms and other palm species, as well as a major pest of bananas (Musa spp.) in different parts of the world. Recently, RPM dispersed throughout the Caribbean islands and has reached both the North and South American continents. The RPM introductions have caused severe damage to palm species, and bananas and plantains in the Caribbean region. The work presented herein is the result of several acaricide trials conducted in Puerto Rico and Florida on palms and bananas in order to provide chemical control alternatives to minimize the impact of this pest. Spiromesifen, dicofol and acequinocyl were effective in reducing the population of R. indica in coconut in Puerto Rico. Spray treatments with etoxanole, abamectin, pyridaben, milbemectin and sulfur showed mite control in Florida. In addition, the acaricides acequinocyl and spiromesifen were able to reduce the population of R. indica in banana trials.

  6. Production of bioethanol using agricultural waste: banana pseudo stem

    Directory of Open Access Journals (Sweden)

    Snehal Ingale

    2014-09-01

    Full Text Available India is amongst the largest banana (Musa acuminata producing countries and thus banana pseudo stem is commonly available agricultural waste to be used as lignocellulosic substrate. Present study focuses on exploitation of banana pseudo stem as a source for bioethanol production from the sugars released due to different chemical and biological pretreatments. Two fungal strains Aspergillus ellipticus and Aspergillus fumigatus reported to be producing cellulolytic enzymes on sugarcane bagasse were used under co-culture fermentation on banana pseudo stem to degrade holocellulose and facilitate maximum release of reducing sugars. The hydrolysate obtained after alkali and microbial treatments was fermented by Saccharomyces cerevisiae NCIM 3570 to produce ethanol. Fermentation of cellulosic hydrolysate (4.1 g% gave maximum ethanol (17.1 g/L with yield (84% and productivity (0.024 g%/h after 72 h. Some critical aspects of fungal pretreatment for saccharification of cellulosic substrate using A. ellipticus and A. fumigatus for ethanol production by S. cerevisiae NCIM 3570 have been explored in this study. It was observed that pretreated banana pseudo stem can be economically utilized as a cheaper substrate for ethanol production.

  7. Production of bioethanol using agricultural waste: banana pseudo stem.

    Science.gov (United States)

    Ingale, Snehal; Joshi, Sanket J; Gupte, Akshaya

    2014-01-01

    India is amongst the largest banana (Musa acuminata) producing countries and thus banana pseudo stem is commonly available agricultural waste to be used as lignocellulosic substrate. Present study focuses on exploitation of banana pseudo stem as a source for bioethanol production from the sugars released due to different chemical and biological pretreatments. Two fungal strains Aspergillus ellipticus and Aspergillus fumigatus reported to be producing cellulolytic enzymes on sugarcane bagasse were used under co-culture fermentation on banana pseudo stem to degrade holocellulose and facilitate maximum release of reducing sugars. The hydrolysate obtained after alkali and microbial treatments was fermented by Saccharomyces cerevisiae NCIM 3570 to produce ethanol. Fermentation of cellulosic hydrolysate (4.1 g%) gave maximum ethanol (17.1 g/L) with yield (84%) and productivity (0.024 g%/h) after 72 h. Some critical aspects of fungal pretreatment for saccharification of cellulosic substrate using A. ellipticus and A. fumigatus for ethanol production by S. cerevisiae NCIM 3570 have been explored in this study. It was observed that pretreated banana pseudo stem can be economically utilized as a cheaper substrate for ethanol production.

  8. Within-plant distribution and binomial sampling of Pentalonia nigronervosa (Hemiptera: Aphididae) on banana.

    Science.gov (United States)

    Robson, Jacqueline D; Wright, Mark G; Almeida, Rodrigo P P

    2006-12-01

    The banana aphid, Pentalonia nigronervosa Coquerel (Hemiptera: Aphididae), infests banana (Musa spp.) worldwide. Pentalonia nigronervosa is the vector of Banana bunchy top virus (family Nanoviridae, genus Babuvirus) the etiological agent of Banana bunchy top disease (BBTD). BBTD is currently the most serious problem affecting banana in Hawaii. Despite the importance of this vector species, little is known about its biology or ecology. There are also no sampling plans available for P. nigronervosa. We conducted field surveys to develop a sampling plan for this pest. Ten plots were surveyed on seven commercial banana farms on the island of Oahu, HI, for the presence of P. nigronervosa on banana plantlets. We found aphids more frequently near the base of plants, followed by the newest unfurled leaf at the top of the plant. Aphids were least likely to be located on leaves in between the top and bottom of the plant. Aphid infestation on surveyed plots ranged from 8 to 95%. We developed a sequential binomial sampling plan based on our surveys. We also discovered that the within-plant distribution of P. nigronervosa is an important factor to consider when sampling for this pest. Our sampling plan will assist in the development of sustainable management practices for banana production.

  9. Expression of sweet pepper Hrap gene in banana enhances resistance to Xanthomonas campestris pv. musacearum.

    Science.gov (United States)

    Tripathi, Leena; Mwaka, Henry; Tripathi, Jaindra Nath; Tushemereirwe, Wilberforce Kateera

    2010-11-01

    Banana Xanthomonas wilt (BXW), caused by the bacterium Xanthomonas campestris pv. musacearum, is the most devastating disease of banana in the Great Lakes region of Africa. The pathogen's rapid spread has threatened the livelihood of millions of Africans who rely on banana fruit for food security and income. The disease is very destructive, infecting all banana varieties, including both East African Highland bananas and exotic types of banana. In the absence of natural host plant resistance among banana cultivars, the constitutive expression of the hypersensitivity response-assisting protein (Hrap) gene from sweet pepper (Capsicum annuum) was evaluated for its ability to confer resistance to BXW. Transgenic lines expressing the Hrap gene under the regulation of the constitutive CaMV35S promoter were generated using embryogenic cell suspensions of two banana cultivars: 'Sukali Ndiizi' and 'Mpologoma'. These lines were characterized by molecular analysis, and were challenged with Xanthomonas campestris pv. musacearum to analyse the efficacy of the Hrap gene against BXW. The majority of transgenic lines (six of eight) expressing Hrap did not show any symptoms of infection after artificial inoculation of potted plants in the screenhouse, whereas control nontransgenic plants showed severe symptoms resulting in complete wilting. This study demonstrates that the constitutive expression of the sweet pepper Hrap gene in banana results in enhanced resistance to BXW. We describe the development of transgenic banana varieties resistant to BXW, which will boost the arsenal available to fight this epidemic disease and save livelihoods in the Great Lakes region of East and Central Africa.

  10. Biology, etiology, and control of virus diseases of banana and plantain.

    Science.gov (United States)

    Kumar, P Lava; Selvarajan, Ramasamy; Iskra-Caruana, Marie-Line; Chabannes, Matthieu; Hanna, Rachid

    2015-01-01

    Banana and plantain (Musa spp.), produced in 10.3 million ha in the tropics, are among the world's top 10 food crops. They are vegetatively propagated using suckers or tissue culture plants and grown almost as perennial plantations. These are prone to the accumulation of pests and pathogens, especially viruses which contribute to yield reduction and are also barriers to the international exchange of germplasm. The most economically important viruses of banana and plantain are Banana bunchy top virus (BBTV), a complex of banana streak viruses (BSVs) and Banana bract mosaic virus (BBrMV). BBTV is known to cause the most serious economic losses in the "Old World," contributing to a yield reduction of up to 100% and responsible for a dramatic reduction in cropping area. The BSVs exist as episomal and endogenous forms are known to be worldwide in distribution. In India and the Philippines, BBrMV is known to be economically important but recently the virus was discovered in Colombia and Costa Rica, thus signaling its spread into the "New World." Banana and plantain are also known to be susceptible to five other viruses of minor significance, such as Abaca mosaic virus, Abaca bunchy top virus, Banana mild mosaic virus, Banana virus X, and Cucumber mosaic virus. Studies over the past 100 years have contributed to important knowledge on disease biology, distribution, and spread. Research during the last 25 years have led to a better understanding of the virus-vector-host interactions, virus diversity, disease etiology, and epidemiology. In addition, new diagnostic tools were developed which were used for surveillance and the certification of planting material. Due to a lack of durable host resistance in the Musa spp., phytosanitary measures and the use of virus-free planting material are the major methods of virus control. The state of knowledge on BBTV, BBrMV, and BSVs, and other minor viruses, disease spread, and control are summarized in this review.

  11. Effect of enzymatic clarifier complexes Clarex and CEC1-CTAA on the quality of banana juice

    OpenAIRE

    Marisa Helena Cardoso; Hilary Castle de Menezes; Marisa de Nazaré Hoelz Jackix; Elisabeth Borges Gonçalves

    1999-01-01

    Neste trabalho foi observado o efeito dos complexos enzimáticos clarificantes Clarex e CEC1-CTAA, adicionados na proporção de 0,03% v/p sobre purê de banana (Musa cavendishii), em condições amenas de hidrólise (40ºC, 15 minutos) visando determinar a qualidade, aqui representada pelos indicadores: rendimento; viscosidade; Brix; pH; composição centesimal; contagens de bolores e leveduras e de mesófilos, e propriedades sensoriais de cor, aroma, sabor e corpo dos sucos de banana clarificados. O s...

  12. Citric acid production by Koji fermentation using banana peel as a novel substrate.

    Science.gov (United States)

    Karthikeyan, Alagarsamy; Sivakumar, Nallusamy

    2010-07-01

    The growing demand for citric acid and the current need for alternative sources have encouraged biotechnologists to search for novel and economical substrates. Koji fermentation was conducted using the peels of banana (Musa acuminata) as an inexpensive substrate for the production of citric acid using Aspergillus niger. Various crucial parameters that affect citric acid production such as moisture content, temperature, pH, inoculum level and incubation time were quantified. Moisture (70%), 28 degrees C temperature, an initial pH 3, 10(8) spores/ml as inoculum and 72h incubation was found to be suitable for maximum citric acid production by A. niger using banana peel as a substrate.

  13. In vitro colonic fermentation and glycemic response of different kinds of unripe banana flour.

    Science.gov (United States)

    Menezes, Elizabete Wenzel; Dan, Milana C T; Cardenette, Giselli H L; Goñi, Isabel; Bello-Pérez, Luis Arturo; Lajolo, Franco M

    2010-12-01

    This work aimed to study the in vitro colonic fermentation profile of unavailable carbohydrates of two different kinds of unripe banana flour and to evaluate their postprandial glycemic responses. The unripe banana mass (UBM), obtained from the cooked pulp of unripe bananas (Musa acuminata, Nanicão variety), and the unripe banana starch (UBS), obtained from isolated starch of unripe banana, plantain type (Musa paradisiaca) in natura, were studied. The fermentability of the flours was evaluated by different parameters, using rat inoculum, as well as the glycemic response produced after the ingestion by healthy volunteers. The flours presented high concentration of unavailable carbohydrates, which varied in the content of resistant starch, dietary fiber and indigestible fraction (IF). The in vitro colonic fermentation of the flours was high, 98% for the UBS and 75% for the UBM when expressed by the total amount of SCFA such as acetate, butyrate and propionate in relation to lactulose. The increase in the area under the glycemic curve after ingestion of the flours was 90% lower for the UBS and 40% lower for the UBM than the increase produced after bread intake. These characteristics highlight the potential of UBM and UBS as functional ingredients. However, in vivo studies are necessary in order to evaluate the possible benefit effects of the fermentation on intestinal health.

  14. Let's Go Bananas.

    Science.gov (United States)

    Brown, Helen; And Others

    1995-01-01

    Presents a hands-on primary science unit of activities designed to teach students concepts about bananas. Real bananas are used as students investigate and use the process skills of observation, measurement, and communication. Using bananas as a theme, science, mathematics, social studies, music, and writing are integrated into the curriculum of…

  15. Thermotherapy, chemotherapy, and meristem culture in banana.

    Science.gov (United States)

    Lassois, Ludivine; Lepoivre, Philippe; Swennen, Rony; van den Houwe, Ines; Panis, Bart

    2013-01-01

    Bananas that provide a staple food to the millions of people are adversely affected by several viruses such as Banana bunchy Top Virus (BBTV), Banana Streak Virus (BSV), and Cucumber Mosaic Virus (CMV). These viruses are known to have a devastating effect on crop production and constraint to the international exchange and conservation of banana germplasm-a cornerstone for breeding new cultivars. The viruses are particularly problematic in vegetative propagated crops, like bananas, because of their transmission in the planting material. Different virus eradication techniques have been developed, such as thermotherapy, chemotherapy, and meristem culture for providing virus-free planting material. Meristem culture proved to be the most effective procedure to eradicate phloem-associated viruses. This method requires isolation of meristematic dome of plant under the aseptic conditions and culture in an appropriate nutrient medium to develop new virus-free plants. Thermotherapy is another widely used virus eradication technique, which is initially carried out on in vivo or in vitro plants and eventually combined with meristem culture technique. The plantlets are initially grown at 28°C day temperature and increase it by 2°C per day until reaches 40°C and the night temperature at 28°C; maintain plants at 40°C for 4 weeks; excise meristem and culture onto the regeneration medium. In chemotherapy technique, antiviral chemical compound Virazole(®) is applied on meristem culture. Combination of these techniques is also applied to improve the eradication rate.

  16. Ripening influences banana and plantain peels composition and energy content

    OpenAIRE

    Happi Emaga, Thomas; Bindelle, Jérôme; Angeesens, Richard; Buldgen, André; Wathelet, Bernard; Paquot, Michel

    2011-01-01

    Musa sp. peels are widely used by smallholders as complementary feeds for cattle in the tropics. A study of the influence of the variety and the maturation stage of the fruit on fermentability and metabolisable energy (ME) content of the peels was performed using banana (Yangambi Km5) and plantain (Big Ebanga) peels at three stages of maturation in an in vitro model of the rumen. Peel samples were analysed for starch, free sugars and fibre composition. Samples were incubated in the presence o...

  17. Valorization and Miscellaneous Prospects of Waste Musa balbisiana Colla Pseudostem

    Directory of Open Access Journals (Sweden)

    Krishna Gogoi

    2014-01-01

    Full Text Available Resourceful utilization of the enormous quantum of agrowastes generated via agricultural practices can be supportive in waste management, environmental upgradation, and subsequent material and energy recovery. In this regard, the present study aimed at highlighting waste banana (Musa balbisiana Colla pseudostem (an agrowaste as a potential bio-based feedstock with miscellaneous applications. The pseudostem was characterized by carbon, nitrogen, and hydrogen (CHN analysis, thermogravimetric-differential thermal analysis (TGDTA, and Fourier transform infrared (FTIR spectroscopy. Cellulose, hemicellulose, and lignin were estimated as a part of biochemical characterization. Total phenolic content, total flavonoid content, 1, 1-diphenyl-2-picrylhydrazyl (DPPH radical scavenging assay, and ferric reducing antioxidant power (FRAP were carried out as a part of antioxidant characterization. The waste banana pseudostem biomass (WBPB was also tried successfully as a natural filler in polyvinyl chloride (PVC polymer composite. Thermal properties and water uptake test of the WBPB polymer composite were accessed as a part of composite characterization. The pseudostem had calorific value (15.22 MJ/kg, high holocellulose (58.67%, high free radical scavenging potential (69.9%, and a low ash content (6.8%. Additionally, the WBPB polymer composite showed improved water resistance and thermostability. The study suggests feasibility of WBPB as a prospective bioenergy feedstock, primary antioxidant source, and reinforcing agent in polymer composites.

  18. Indigenous anti-ulcer activity of Musa sapientum on peptic ulcer

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    P Prabha

    2011-01-01

    Full Text Available Background: Peptic ulcer disease (PUD, encompassing gastric and duodenal ulcers is the most prevalent gastrointestinal disorder. The pathophysiology of PUD involves an imbalance between offensive factors like acid, pepsin and defensive factors like nitric oxide and growth factors. The clinical evaluation of antiulcer drugs showed tolerance, incidence of relapses and side-effects that make their efficacy arguable. An indigenous drug like Musa sapientum possessing fewer side-effects is the major thrust area of present day research, aiming at a better and safer approach for the management of PUD. Material and Methods: The unripe plantain bananas (Musa sapientum were shade-dried, powdered and used for phytochemical analysis and as antiulcer drug. In our present study Group I rats served as control and were treated with saline, Group II was indomethacin-induced ulcerated rats, Group III received aqueous extract of Musa sapientum along with indomethacin and Group IV received esomeprazole along with indomethacin for 21 days. The anti-ulcerogenic activity was investigated by performing hematological, mucosal, antioxidant profile in comparison with the standard drug esomeprazole. Results: Our findings from High - Performance Thin Layer Chromatography (HPTLC analysis showed that Musa sapientum has an active compound a monomeric flavonoid (leucocyanidin with anti-ulcerogenic activity. Results were expressed as mean ± SD. All our results are in congruous with the results of standard drug esomeprazole. Conclusion: It could be clearly concluded that administration of the aqueous extract of Musa sapientum at the dose used in this study tends to ameliorate ulcers. Its use in indigenous medicine should be scientifically scrutinized with further research.

  19. The influence of alkali treatment on banana fibre’s mechanical properties

    Directory of Open Access Journals (Sweden)

    Julio César Mejía Osorio

    2012-04-01

    Full Text Available This work analyses the effect of alkali treatment on the mechanical properties of banana fibre (Musa Paradisiaca. Fibres were extracted from the pseudostem by a defibring machine; they were mercerised and modified by 5% NaOH (w/v alkali treatment. Morphological characterisation showed that treated fibres’ surface was rougher than that of untreated fibres. Mechanical characterisation indicated that Young’s modulus, ultimate tensile strength and strain became decreased by increasing both treated and untreated fibres’ diameter.

  20. 不同香蕉品种多聚半乳糖醛酸酶抑制蛋白活性的比较%Comparison of the Activity of Polygalacturonase Inhibiting Proteins in Different Banana Varieties

    Institute of Scientific and Technical Information of China (English)

    刘昆昂; 王振中

    2013-01-01

    Polygalacturonase inhibiting proteins (PGIP), containing rich leucine with repeated region, can inhibit the activity of a variety of fungal Polygalacturonases (PG), and is considered as a kind of important factor in plant resistance against fungal infection. In this study, we used several banana varieties as the research materials to detect their PGIP activities to inhibit PGs produced by race 4 of Fusarium oxysporum f.sp. cubense (Foc4), the severe disease pathogen of banana fusarium wilt. Those banana varieties were concerned as resistant varieties to the pathogen and with different cell genotypes, including Musa AAA, Musa ABB, Musa ABB, Musa AA and Musa AAAB. The results showed that PGIP from Dongguandajiao (Musa ABB) inhibitted PGs of Foc4 with the highest activity among these banana varieties. By comparing the PGIP activities in roots, pseudo-stems and leaves of Dongguandajiao, PGIP from pseudo-stem was found with the highest PGIP activity. The study also found that the PGIP activity could be induced by Foc4 inoculation, and PGIP activity was higher in young seedlings than that in older ones.%植物多聚半乳糖醛酸酶抑制蛋白(PGIP)是一种富含亮氨酸重复区域的蛋白质,能够非竞争性地抑制真菌的多聚半乳糖醛酸酶(PG)的活性.以目前生产上几个主要抗枯萎病的香蕉品种为材料,参照Gross的PGIP活性测定方法,通过测定了Musa AAA、Musa ABB、Musa ABB、Musa AA和Musa AAAB等不同细胞基因型的香蕉品种的PGIP活性,发现东莞大蕉(Musa ABB)的活性最高.通过对东莞大蕉根、假茎、叶不同部位的PGIP活性比较,发现假茎的活性最高.用香蕉枯萎病菌4号生理小种接种诱导东莞大蕉能明显提高PGIP活性,观察还发现东莞大蕉苗期PGIP的活性明显高于后期.

  1. Cooking Banana Consumption Patterns in the Plantain-growing Area of Southeastern Nigeria

    Directory of Open Access Journals (Sweden)

    Tshiunza, M.

    2001-01-01

    Full Text Available Cooking bananas (Musa spp., ABB genome were intro-duced into Southeastern Nigeria by the International Institute of Tropical Agriculture (IITA in the mid-1980s as an interim measure to reduce the incidence of black sigatoka disease (caused by the fungus Mycosphaerel-la fijiensis Morelet on plantain. However, the people of this region were not familiar with their utilisation methods. To address this lack of the knowledge and thereby sustain cooking banana cultivation, IITA, in collaboration with the Shell Petroleum Development Company (SPDC and the Nigeria Agip OU Company (NAOC commenced a training campaign on cooking banana processing methods. This study examined the patterns of utilisation of cooking bananas ten years after the training took place and compared them with plantain. About 95 % of the households interviewed are consuming cooking banana, indicating a broad acceptance of the crop in the region. Overall, two ripening stages termed green and ripe are the most popular ripening stages for the consumption of both plantain and cooking banana, followed by partially ripe maturity stage. The most common forms of consumption for green plantain are, in decreasing order of importance, pottage, boiled, roasted, and fried. Green cooking banana is also mostly eaten in pottage and boiled forms, and less frequently in fried and pounded forms. Ripe plantain is mostly eaten in fried and pottage forms, while ripe cooking banana is mostly eaten in fried and raw forms. Partially ripe plantain is mostly eaten in pottage, fried, boiled, and roasted forms, while partially ripe cooking banana is eaten in fried, pottage and boiled forms. These results indicate that the consumption patterns of plantain and cooking banana are very similar. This similarity has greatly contributed to the rapid integration of cooking banana within the existing plantain consumption and cropping systems.

  2. Structural and Function Prediction of Musa acuminata subsp. Malaccensis Protein

    Directory of Open Access Journals (Sweden)

    Anum Munir

    2016-03-01

    Full Text Available Hypothetical proteins (HPs are the proteins whose presence has been anticipated, yet in vivo function has not been built up. Illustrating the structural and functional privileged insights of these HPs might likewise prompt a superior comprehension of the protein-protein associations or networks in diverse types of life. Bananas (Musa acuminata spp., including sweet and cooking types, are giant perennial monocotyledonous herbs of the order Zingiberales, a sister grouped to the all-around considered Poales, which incorporate oats. Bananas are crucial for nourishment security in numerous tropical and subtropical nations and the most prominent organic product in industrialized nations. In the present study, the hypothetical protein of M. acuminata (Banana was chosen for analysis and modeling by distinctive bioinformatics apparatuses and databases. As indicated by primary and secondary structure analysis, XP_009393594.1 is a stable hydrophobic protein containing a noteworthy extent of α-helices; Homology modeling was done utilizing SWISS-MODEL server where the templates identity with XP_009393594.1 protein was less which demonstrated novelty of our protein. Ab initio strategy was conducted to produce its 3D structure. A few evaluations of quality assessment and validation parameters determined the generated protein model as stable with genuinely great quality. Functional analysis was completed by ProtFun 2.2, and KEGG (KAAS, recommended that the hypothetical protein is a transcription factor with cytoplasmic domain as zinc finger. The protein was observed to be vital for translation process, involved in metabolism, signaling and cellular processes, genetic information processing and Zinc ion binding. It is suggested that further test approval would help to anticipate the structures and functions of other uncharacterized proteins of different plants and living being.

  3. Nitrogen and potassium fertilization on 'Caipira' and 'BRS Princesa' bananas in the Ribeira Valley.

    OpenAIRE

    Edson S. Nomura; Cuquel,Francine L.; Damatto Junior,Erval R.; Eduardo J. Fuzitani; Borges,Ana L.; Saes,Luis A.

    2016-01-01

    ABSTRACT ‘BRS Princesa’ (AAAB) and ‘Caipira’ (AAA) banana cultivars have similar sensorial features in comparison to the ‘Maçã’ banana. They are resistant to Panama disease, which allows them to grow in the Ribeira Valley, the largest banana plantation area in the São Paulo State. However, there is no information on how to fertilize crop under these edaphoclimatic conditions. This study aimed to evaluate the development and production of ‘Caipira’ and ‘BRS Princesa’ bananas, by applying four ...

  4. Efeito do ensacamento de cachos de banana 'Nanicão' na produção e no intervalo entre inflorescência e colheita

    Directory of Open Access Journals (Sweden)

    Costa José Nilton Medeiros

    2002-01-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito do ensacamento de cachos de bananas (Musa sp. AAA, cultivar Nanicão, em diferentes épocas, na produção e no intervalo entre inflorescência e colheita. O experimento foi realizado no Município de Tietê, SP, em blocos ao acaso, em esquema fatorial 2x3, com quatro repetições. O ensacamento foi efetuado com sacos de polietileno em três épocas: 21/5/96, 17/12/96 e 26/2/97. Foram avaliados o intervalo entre emergência da inflorescência e colheita, a massa do cacho e o comprimento, diâmetro e densidade do fruto. A interação ensacamento x épocas não foi significativa em relação a nenhuma das variáveis. O ensacamento diminuiu o intervalo entre a emergência da inflorescência e a colheita na primeira (21/5/96 e segunda época (17/12/96.

  5. Nitrogen and potassium fertilization on ‘Caipira’ and ‘BRS Princesa’ bananas in the Ribeira Valley

    OpenAIRE

    Edson S. Nomura; Cuquel,Francine L.; Damatto Junior,Erval R.; Eduardo J. Fuzitani; Borges,Ana L.; Saes,Luis A.

    2016-01-01

    ABSTRACT ‘BRS Princesa’ (AAAB) and ‘Caipira’ (AAA) banana cultivars have similar sensorial features in comparison to the ‘Maçã’ banana. They are resistant to Panama disease, which allows them to grow in the Ribeira Valley, the largest banana plantation area in the São Paulo State. However, there is no information on how to fertilize crop under these edaphoclimatic conditions. This study aimed to evaluate the development and production of ‘Caipira̵...

  6. Severity of banana leaf spot in an intercropping system in two cycles of banana Prata Anã

    Directory of Open Access Journals (Sweden)

    Valdeir Dias Gonçalves

    2008-01-01

    Full Text Available Prata Anã is the most planted banana cultivar in northern Minas Gerais, Brazil. It is however susceptible toseveral pathogens. This study was carried out to evaluate the disease severity of banana leaf spot in the Prata Anã cv. in thefirst and second cycle under six different planting systems. The randomized block experimental design was used with sixtreatments and four replications. In an evaluation of the severity of banana leaf spot, no disease symptoms were found onThap Maeo and Caipira. The evolution curve of the disease indicated seasonal effects in the first and second cycles. Theseverity of banana leaf spot was highest soon after the regional rainy period from November to March. A comparison of themeans of the evaluations indicated a reduction in disease severity from the first to the second cycle.

  7. Bioactive compounds in banana and their associated health benefits - A review.

    Science.gov (United States)

    Singh, Balwinder; Singh, Jatinder Pal; Kaur, Amritpal; Singh, Narpinder

    2016-09-01

    Banana is a very popular fruit in the world market and is consumed as staple food in many countries. It is grown worldwide and constitutes the fifth most important agricultural food crop in terms of world trade. It has been classified into the dessert or sweet bananas and the cooking bananas or plantains. It is either eaten raw or processed, and also as a functional ingredient in various food products. Banana contains several bioactive compounds, such as phenolics, carotenoids, biogenic amines and phytosterols, which are highly desirable in the diet as they exert many positive effects on human health and well-being. Many of these compounds have antioxidant activities and are effective in protecting the body against various oxidative stresses. In the past, bananas were effectively used in the treatment of various diseases, including reducing the risk of many chronic degenerative disorders. In the present review, historical background, cultivar classification, beneficial phytochemicals, antioxidant activity and health benefits of bananas are discussed.

  8. Gamma radiation effects on the viscosity of green banana flour

    Energy Technology Data Exchange (ETDEWEB)

    Uehara, Vanessa B.; Inamura, Patricia Y.; Mastro, Nelida L. Del [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)], e-mail: vanessa.uehara@usp.br, e-mail: patyoko@yahoo.com, e-mail: nlmastro@ipen.br

    2009-07-01

    Banana (Musa sp) is a tropical fruits with great acceptability among consumers and produced in Brazil in a large scale. Bananas are not being as exploited as they could be in prepared food, and research could stimulate greater interest from industry. The viscosity characteristics and a product consistency can determine its acceptance by the consumer. Particularly the starch obtained from green banana had been studied from the nutritional point of view since the concept of Resistant Starch was introduced. Powder RS with high content of amylose was included in an approved food list with alleged functional properties in Brazilian legislation. Ionizing radiation can be used as a public health intervention measure for the control of food-borne diseases. Radiation is also a very convenient tool for polymer materials modification through degradation, grafting and crosslinking. In this work the influence of ionizing radiation on the rheological behavior of green banana pulp was investigated. Samples of green banana pulp flour were irradiated in a {sup 60}Co Gammacell 220 (AECL) with doses of 0 kGy,1 kGy, 3 kGy, 5 kGy and 10 kGy in glass recipients. After irradiation 3% and 5% aqueous dilution were prepared and viscosity measurements performed in a Brooksfield, model DVIII viscometer using spindle SC4-18 and SC4-31. There was a reduction of the initial viscosity of the samples as a consequence of radiation processing, being the reduction inversely proportional to the flour concentration. The polysaccharide content of the banana starch seems to be degraded by radiation in solid state as shown by the reduction of viscosity as a function of radiation dose. (author)

  9. Efeito do 1-metilciclopropeno sobre a emissão dos ésteres voláteis de bananas ao longo do amadurecimento Efferct of 1-MCP on esters volatiles eission of bananas along the ripening

    Directory of Open Access Journals (Sweden)

    Baraquizio Braga do Nascimento Junior

    2008-01-01

    Full Text Available Fresh green bananas (Musa sp., subgroup Prata were treated with a dose of only 90 ηg g-1 of 1-MCP for 13 hours and the evolution of the volatile compounds along the ripeness was studied. A method to quantify the emission of esters was developed by cryogenic headspace and gas chromatography. Esters of acetate, butyrate, isobutyrate and isovalerate were found as major compounds. The application of the 1-MCP for 13 hours delayed the appearance of the coloration 8 of the peel for 3 days and decreased quantitatively in about 46% the total production of esters in the banana until the 15° day of harvested.

  10. La academia poética Musa Musae

    Directory of Open Access Journals (Sweden)

    Federico Utrera

    2012-03-01

    Full Text Available La academia poética Musa Musae, primera tertulia cultural de calado formada tras la guerra civil española, pues abrió sus puertas el 17 de enero de 1940, diez meses y medio después de finalizada oficialmente la contienda (1 de abril de 1939, fue un tímido y frustrado intento por sentar las bases de una futura reconciliación nacional sobre utópicos y románticos presupuestos literarios y artísticos. Denostada por sus críticos, en este artículo se fija y contextualiza su aparición y participantes, se reúnen diferentes testimonios de asistentes, algunos de ellos inéditos o poco conocidos, y se definen sus principales características como la pionera iniciativa literaria de los sectores más aperturistas y conciliadores del régimen, sucediéndole la revista Escorial, las tertulias del Café Gijón y el suplemento cultural del diario Arriba en una España destruida y con sus heridas bélicas aún abiertas, pues su exilio percibía todos estos movimientos con bastante escepticismo.

  11. Efeito dos complexos enzimaticos clarificantes clarex e CEC1-CTAA sobre a qualidade do suco de banana.

    OpenAIRE

    Cardoso,Marisa Helena; Menezes,Hilary Castle de; Jackix, Marisa de Nazaré Hoelz; GONÇALVES,Elisabeth Borges

    1999-01-01

    Neste trabalho foi observado o efeito dos complexos enzimáticos clarificantes Clarex e CEC1-CTAA, adicionados na proporção de 0,03% v/p sobre purê de banana (Musa cavendishii), em condições amenas de hidrólise (40ºC, 15 minutos) visando determinar a qualidade, aqui representada pelos indicadores: rendimento; viscosidade; Brix; pH; composição centesimal; contagens de bolores e leveduras e de mesófilos, e propriedades sensoriais de cor, aroma, sabor e corpo dos sucos de banana clarificados. O s...

  12. Bananas go paraelectric

    Energy Technology Data Exchange (ETDEWEB)

    Loidl, A; Krohns, S; Hemberger, J; Lunkenheimer, P [Experimental Physics V, Center for Electronic Correlations and Magnetism, University of Augsburg, 86159 Augsburg (Germany)], E-mail: peter.lunkenheimer@physik.uni-augsburg.de

    2008-05-14

    Using a banana as an example, we demonstrate how the ferroelectric-like hysteresis loops measured in inhomogeneous, conducting materials can easily be identified as non-intrinsic. With simple experiments, the response of a banana to electric fields is revealed as characteristic for an inhomogeneous paraelectric ion conductor. Not even absolute beginners in dielectrics should identify this biological matter as ferroelectric. (viewpoint)

  13. Anaphylaxis caused by banana.

    Science.gov (United States)

    Savonius, B; Kanerva, L

    1993-04-01

    An anaphylactic reaction following ingestion of banana occurred in a 32-year-old female cook. The sensitization to banana occurred simultaneously with the development of occupational asthma caused by grain flour. The patient was sensitized to a wide range of airborne and ingestible proteins but not to rubber latex.

  14. Agroforestry leads to shifts within the gammaproteobacterial microbiome of banana plants cultivated in Central America.

    Science.gov (United States)

    Köberl, Martina; Dita, Miguel; Martinuz, Alfonso; Staver, Charles; Berg, Gabriele

    2015-01-01

    Bananas (Musa spp.) belong to the most important global food commodities, and their cultivation represents the world's largest monoculture. Although the plant-associated microbiome has substantial influence on plant growth and health, there is a lack of knowledge of the banana microbiome and its influencing factors. We studied the impact of (i) biogeography, and (ii) agroforestry on the banana-associated gammaproteobacterial microbiome analyzing plants grown in smallholder farms in Nicaragua and Costa Rica. Profiles of 16S rRNA genes revealed high abundances of Pseudomonadales, Enterobacteriales, Xanthomonadales, and Legionellales. An extraordinary high diversity of the gammaproteobacterial microbiota was observed within the endophytic microenvironments (endorhiza and pseudostem), which was similar in both countries. Enterobacteria were identified as dominant group of above-ground plant parts (pseudostem and leaves). Neither biogeography nor agroforestry showed a statistically significant impact on the gammaproteobacterial banana microbiome in general. However, indicator species for each microenvironment and country, as well as for plants grown in Coffea intercropping systems with and without agri-silvicultural production of different Fabaceae trees (Inga spp. in Nicaragua and Erythrina poeppigiana in Costa Rica) could be identified. For example, banana plants grown in agroforestry systems were characterized by an increase of potential plant-beneficial bacteria, like Pseudomonas and Stenotrophomonas, and on the other side by a decrease of Erwinia. Hence, this study could show that as a result of legume-based agroforestry the indigenous banana-associated gammaproteobacterial community noticeably shifted.

  15. Fluorescent chlorophyll catabolites in bananas light up blue halos of cell death

    Science.gov (United States)

    Moser, Simone; Müller, Thomas; Holzinger, Andreas; Lütz, Cornelius; Jockusch, Steffen; Turro, Nicholas J.; Kräutler, Bernhard

    2009-01-01

    Breakdown of chlorophyll is a major contributor to the diagnostic color changes in fall leaves, and in ripening apples and pears, where it commonly provides colorless, nonfluorescent tetrapyrroles. In contrast, in ripening bananas (Musa acuminata) chlorophylls fade to give unique fluorescent catabolites (FCCs), causing yellow bananas to glow blue, when observed under UV light. Here, we demonstrate the capacity of the blue fluorescent chlorophyll catabolites to signal symptoms of programmed cell death in a plant. We report on studies of bright blue luminescent rings on the peel of very ripe bananas, which arise as halos around necrotic areas in ‘senescence associated’ dark spots. These dark spots appear naturally on the peel of ripe bananas and occur in the vicinity of stomata. Wavelength, space, and time resolved fluorescence measurements allowed the luminescent areas to be monitored on whole bananas. Our studies revealed an accumulation of FCCs in luminescent rings, within senescing cells undergoing the transition to dead tissue, as was observable by morphological textural cellular changes. FCCs typically are short lived intermediates of chlorophyll breakdown. In some plants, FCCs are uniquely persistent, as is seen in bananas, and can thus be used as luminescent in vivo markers in tissue undergoing senescence. While FCCs still remain to be tested for their own hypothetical physiological role in plants, they may help fill the demand for specific endogenous molecular reporters in noninvasive assays of plant senescence. Thus, they allow for in vivo studies, which provide insights into critical stages preceding cell death. PMID:19805212

  16. Agroforestry leads to shifts within the gammaproteobacterial microbiome of banana plants cultivated in Central America

    Directory of Open Access Journals (Sweden)

    Martina eKöberl

    2015-02-01

    Full Text Available Bananas (Musa spp. belong to the most important global food commodities, and their cultivation represents the world’s largest monoculture. Although the plant-associated microbiome has substantial influence on plant growth and health, there is a lack of knowledge of the banana microbiome and its influencing factors. We studied the impact of i biogeography, and ii agroforestry on the banana-associated gammaproteobacterial microbiome analyzing plants grown in smallholder farms in Nicaragua and Costa Rica. Profiles of 16S rRNA genes revealed high abundances of Pseudomonadales, Enterobacteriales, Xanthomonadales, and Legionellales. An extraordinary high diversity of the gammaproteobacterial microbiota was observed within the endophytic microenvironments (endorhiza and pseudostem, which was similar in both countries. Enterobacteria were identified as dominant group of above-ground plant parts (pseudostem and leaves. Neither biogeography nor agroforestry showed a statistically significant impact on the gammaproteobacterial banana microbiome in general. However, indicator species for each microenvironment and country, as well as for plants grown in Coffea intercropping systems with and without agri-silvicultural production of different Fabaceae trees (Inga spp. in Nicaragua and Erythrina poeppigiana in Costa Rica could be identified. For example, banana plants grown in agroforestry systems were characterized by an increase of potential plant-beneficial bacteria, like Pseudomonas and Stenotrophomonas, and on the other side by a decrease of Erwinia. Hence, this study could show that as a result of legume-based agroforestry the indigenous banana-associated gammaproteobacterial community noticeably shifted.

  17. Traditional Banana Diversity in Oceania: An Endangered Heritage.

    Science.gov (United States)

    Kagy, Valérie; Wong, Maurice; Vandenbroucke, Henri; Jenny, Christophe; Dubois, Cécile; Ollivier, Anthony; Cardi, Céline; Mournet, Pierre; Tuia, Valérie; Roux, Nicolas; Doležel, Jaroslav; Perrier, Xavier

    2016-01-01

    This study aims to understand the genetic diversity of traditional Oceanian starchy bananas in order to propose an efficient conservation strategy for these endangered varieties. SSR and DArT molecular markers are used to characterize a large sample of Pacific accessions, from New Guinea to Tahiti and Hawaii. All Pacific starchy bananas are shown of New Guinea origin, by interspecific hybridization between Musa acuminata (AA genome), more precisely its local subspecies M. acuminata ssp. banksii, and M. balbisiana (BB genome) generating triploid AAB Pacific starchy bananas. These AAB genotypes do not form a subgroup sensu stricto and genetic markers differentiate two subgroups across the three morphotypes usually identified: Iholena versus Popoulu and Maoli. The Popoulu/Maoli accessions, even if morphologically diverse throughout the Pacific, cluster in the same genetic subgroup. However, the subgroup is not strictly monophyletic and several close, but different genotypes are linked to the dominant genotype. One of the related genotypes is specific to New Caledonia (NC), with morphotypes close to Maoli, but with some primitive characters. It is concluded that the diffusion of Pacific starchy AAB bananas results from a series of introductions of triploids originating in New Guinea area from several sexual recombination events implying different genotypes of M. acuminata ssp. banksii. This scheme of multiple waves from the New Guinea zone is consistent with the archaeological data for peopling of the Pacific. The present geographic distribution suggests that a greater diversity must have existed in the past. Its erosion finds parallels with the erosion of cultural traditions, inexorably declining in most of the Polynesian or Melanesian Islands. Symmetrically, diversity hot spots appear linked to the local persistence of traditions: Maoli in New Caledonian Kanak traditions or Iholena in a few Polynesian islands. These results will contribute to optimizing the

  18. Traditional Banana Diversity in Oceania: An Endangered Heritage

    Science.gov (United States)

    Kagy, Valérie; Wong, Maurice; Vandenbroucke, Henri; Jenny, Christophe; Dubois, Cécile; Ollivier, Anthony; Cardi, Céline; Mournet, Pierre; Tuia, Valérie; Roux, Nicolas; Doležel, Jaroslav; Perrier, Xavier

    2016-01-01

    This study aims to understand the genetic diversity of traditional Oceanian starchy bananas in order to propose an efficient conservation strategy for these endangered varieties. SSR and DArT molecular markers are used to characterize a large sample of Pacific accessions, from New Guinea to Tahiti and Hawaii. All Pacific starchy bananas are shown of New Guinea origin, by interspecific hybridization between Musa acuminata (AA genome), more precisely its local subspecies M. acuminata ssp. banksii, and M. balbisiana (BB genome) generating triploid AAB Pacific starchy bananas. These AAB genotypes do not form a subgroup sensu stricto and genetic markers differentiate two subgroups across the three morphotypes usually identified: Iholena versus Popoulu and Maoli. The Popoulu/Maoli accessions, even if morphologically diverse throughout the Pacific, cluster in the same genetic subgroup. However, the subgroup is not strictly monophyletic and several close, but different genotypes are linked to the dominant genotype. One of the related genotypes is specific to New Caledonia (NC), with morphotypes close to Maoli, but with some primitive characters. It is concluded that the diffusion of Pacific starchy AAB bananas results from a series of introductions of triploids originating in New Guinea area from several sexual recombination events implying different genotypes of M. acuminata ssp. banksii. This scheme of multiple waves from the New Guinea zone is consistent with the archaeological data for peopling of the Pacific. The present geographic distribution suggests that a greater diversity must have existed in the past. Its erosion finds parallels with the erosion of cultural traditions, inexorably declining in most of the Polynesian or Melanesian Islands. Symmetrically, diversity hot spots appear linked to the local persistence of traditions: Maoli in New Caledonian Kanak traditions or Iholena in a few Polynesian islands. These results will contribute to optimizing the

  19. Isolation and Identification of Plantain (Musa paradisiaca Linn.) Fusarium Wilt Disease Pathogen and Pathogenicity Test%大蕉枯萎病病原菌的分离、鉴定和致病性测定

    Institute of Scientific and Technical Information of China (English)

    郭立佳; 彭军; 杨腊英; 王国芬; 梁昌聪; 刘磊; 黄俊生

    2013-01-01

    从表现枯萎病症状的大蕉球茎病组织中获得10株分离物,采用形态学分类方法将这些分离物鉴定为尖孢镰刀菌.利用分离物DJ1的rDNA-ITS区序列和IGS序列开展的系统发育分析迸一步确定DJ1为尖孢镰刀菌.对不同香蕉品系的致病性测定结果表明,DJ1对粉蕉(Musa sp.ABB)的致病性最强,其次是特威(Musa sp.AAA)、巴西蕉(Musa sp.AAA)和泰蕉(Musa sp.AAA),对皇帝蕉(Musa sp.AA)的致病性较弱,根据DJ1的寄主范围确定DJ1为尖孢镰刀菌古巴专化型生理4号小种.对IGS序列的进一步比对分析表明DJ1不属于热带4号生理小种菌株.这些结果为大蕉枯萎病的防治提供了重要依据和指导.%Plantain (Musa paradisiaca linn.) is an edible banana, and the causal agent of plantain wilt disease hasn't been reported until now. In this study, ten isolates (DJ1-10) from the conns of the plantains that showed wilt symptoms were isolated. These isolates were preliminarily identified as Fusarium oxysporm based on their morphological characteristics. Then phylogenetic analysis based on the sequences of rDNA-ITS and intergenic spacer (IGS) regions added proof that the isolate DJ1 was F. oxysporum. The pathogenicity assays showed that DJ1 was able to infect 5 varieties of the genus Musa including Fenjiao (Musa sp. ABB), Hangdijiao (Musa sp. AA), Taijiao (Musa sp. AAA), Tewei (Musa sp. AAA) and Brazilian (Musa sp. AAA), but it was most pathogenic to Fenjiao, then Tewei, Taijiao and Bazilian, and it was less pathogenic to Huangdijiao. These results imply that DJ1 might be an isolate from race 4 of Fusarium oxysporum f. sp. cubense (Foe).

  20. Geographical distribution of Musa gracilis Holttum in Peninsular Malaysia

    Science.gov (United States)

    Norfazlina, B.; Wickneswari, R.; Choong, C. Y.

    2016-11-01

    Musa gracilis (Musaceae) is placed under section Callimusa and was considered endemic to Peninsular Malaysia. The objective of this study was to evaluate the current occurrence of Musa gracilis in Peninsular Malaysia. The coordinates of each population was recorded using the Global Positioning System (GPS) and mapped to show the geographical distribution of Musa gracilis. This study revealed that Musa gracilis exhibits specific pattern of distribution, which exists only in a lowland areas on the eastern and southern part of Peninsular Malaysia.

  1. [Yield of starch extraction from plantain (Musa paradisiaca). Pilot plant study].

    Science.gov (United States)

    Flores-Gorosquera, Emigdia; García-Suárez, Francisco J; Flores-Huicochea, Emmanuel; Núñez-Santiago, María C; González-Soto, Rosalia A; Bello-Pérez, Luis A

    2004-01-01

    In México, the banana (Musa paradisiaca) is cooked (boiling or deep frying) before being eaten, but the consumption is not very popular and a big quantity of the product is lost after harvesting. The unripe plantain has a high level of starch and due to this the use of banana can be diversified as raw material for starch isolation. The objective of this work was to study the starch yield at pilot plant scale. Experiments at laboratory scale were carried out using the pulp with citric acid to 0,3 % (antioxidant), in order to evaluate the different unitary operations of the process. The starch yield, based on starch presence in the pulp that can be isolated, were between 76 and 86 %, and the values at pilot plant scale were between 63 and 71 %, in different lots of banana fruit. Starch yield values were similar among the diverse lots, showing that the process is reproducible. The lower values of starch recovery at pilot plant scale are due to the loss during sieving operations; however, the amount of starch recovery is good.

  2. Ripening influences banana and plantain peels composition and energy content.

    Science.gov (United States)

    Emaga, Thomas Happi; Bindelle, Jérôme; Agneesens, Richard; Buldgen, André; Wathelet, Bernard; Paquot, Michel

    2011-01-01

    Musa sp. peels are widely used by smallholders as complementary feeds for cattle in the tropics. A study of the influence of the variety and the maturation stage of the fruit on fermentability and metabolisable energy (ME) content of the peels was performed using banana (Yangambi Km5) and plantain (Big Ebanga) peels at three stages of maturation in an in vitro model of the rumen. Peel samples were analysed for starch, free sugars and fibre composition. Samples were incubated in the presence of rumen fluid. Kinetics of gas production were modelled, ME content was calculated using prediction equation and short-chain fatty acids production and molar ratio were measured after 72 h of fermentation. Final gas production was higher in plantain (269-339 ml g(-1)) compared to banana (237-328 ml g(-1)) and plantain exhibited higher ME contents (8.9-9.7 MJ/kg of dry matter, DM) compared to banana (7.7-8.8 MJ/kg of DM). Butyrate molar ratio decreased with maturity of the peels. The main influence of the variety and the stage of maturation on all fermentation parameters as well as ME contents of the peels was correlated to changes in the carbohydrate fraction of the peels, including starch and fibre.

  3. Production potentielle de bioéthanol, de biométhane et de pellets à partir des déchets de biomasse lignocellulosique du bananier (Musa spp. au Cameroun

    Directory of Open Access Journals (Sweden)

    Kamdem, I.

    2011-01-01

    Full Text Available Potential production of bioethanol, biomethane and wood pellets from lignocellulosic biomass wastes of the banana plant (Musa spp. in Cameroon. Like most African countries who are producers and exporters of banana, Cameroon is facing a major energy deficit. Yet, the country is generating annually about 4,500,000 tons of fresh banana plant lignocellulosic waste biomass matter equivalent to 402,750 tons of dry matter. The dry matter contained about 80,57% organic matter which are not exploited. Under the sustainable development, which is linked to environmental protection, the biotransformation of these residues can potentially produce about 93,800; 92,133; 447,500 tons of bioethanol, biomethane and pellets respectively. The waste transformation could reduce the energy deficit and create jobs opportunities. Productions of this renewable energy or biofuel also constitute a new area which could assure an important source of income for the banana cultivators and the entire country.

  4. Molecular Characterization of Banana (AA Diploids with Contrasting Levels of Black and Yellow Sigatoka Resistance

    Directory of Open Access Journals (Sweden)

    Claudia F. Ferreira

    2004-01-01

    Full Text Available Most banana cultivars are susceptible to many diseases, whereas Sigatoka leads to greatest yield losses. One of the strategies to overcome this disease is thorough banana genetic breeding which consists in the obtainment of improved (AA diploids which are then crossed with triploids obtaining (AAAB tetraploid disease resistant bananas also presenting other important agronomic characteristics. The prior knowledge of the genetic diversity of (AA diploids, is therefore considered indispensable in order to direct the crosses being made. The objective of the present work was to analyze the genetic diversity of 20 (AA banana diploids with contrasting levels of reaction to yellow and black Sigatoka caused by Mycosphaerella musicola and M. fijensis, respectively, using molecular markers. From the dendrogram data it is shown that a great number of experimental hybrids can be obtained from the combination of genetically different diploids, therefore making the banana genetic breeding program more efficient regarding its objectives.

  5. Host Range and Banana Variety Resistance to Bacterial Soft Rot Pathogens%香蕉细菌性软腐病菌的寄主范围及香蕉品种的抗性测定

    Institute of Scientific and Technical Information of China (English)

    袁月; 陈雪凤; 李华平; 梁家杰; 刘琼光

    2013-01-01

    以分离获得的3个香蕉细菌性软腐病菌为接种菌株,采用针刺和注射等接种方法进行了香蕉细菌性软腐病菌寄主范围的鉴定,并对香蕉不同品种的抗性进行了测定.结果表明,在供试的15科22种植物中有21种植物可以被病原菌侵染而发病,表明该病原菌寄主范围广泛.对我国普遍栽种的7个香蕉品种的抗性测定结果表明,不同香蕉品种抗性存在差异,其中皇帝蕉为高抗品种;农科1号、大蕉和巴西蕉为中抗品种;而金粉、广粉和威廉斯B6为中感品种.%Banana soft rot, a serious disease caused by Dickeya sp., was found in Guangdong and Hainan provinces. The host range of banana soft rot pathogens in China and the disease resistance of different banana varieties were not reported. Three isolates of banana soft rot pathogens and 22 species of plants from 15 families were investigated for pathogen-host range by using artificial inoculation methods. The results indicated that three isolates had a wide host range. In addition, seven banana varieties, which widely grew in China, were used to identify the resistance to banana soft rot. The results showed that Musa AA Pisang Mas had a high resistance; Musa AAA Cavendish cv. Brazil, Musa AAA Cavendish cv. Nongke No. 1, and Musa ABB Pisang had a moderate resistance; Musa ABB Pisang Awak cv. Jinfen, Musa AAA Cavendish cv. Williams, Musa ABB Pisang Awak cv. Guangfen were moderately susceptible.

  6. Marketing de banana: preferências do consumidor quanto aos atributos de qualidade dos frutos Marketing of banana: consumer preferences relating to fruit quality attributes

    Directory of Open Access Journals (Sweden)

    Fernando César Akira Urbano Matsuura

    2004-04-01

    Full Text Available O Brasil produz aproximadamente seis milhões de toneladas por ano de banana (Musa spp., com consumo médio da ordem de 35 kg/ habitante / ano. A aceitação da banana deve-se, principalmente, a seus aspectos sensoriais, valor nutricional e conveniência. A identificação das necessidades e desejos dos clientes consiste em uma atividade crítica do marketing. O objetivo deste trabalho foi o de pesquisar as preferências do consumidor de um mercado local (município de Cruz das Almas - Estado da Bahia considerando os atributos de qualidade dos frutos frescos de banana madura. A metodologia utilizada foi a da pesquisa descritiva por método estatístico. Os dados foram coletados por questionário, na forma de entrevista pessoal com 400 pessoas. Os atributos de qualidade (variáveis questionados e avaliados foram relacionados com a aparência, cor, textura, aroma, sabor e vida útil esperada dos frutos de banana. De acordo com a preferência dos consumidores entrevistados, o fruto de banana maduro ideal deve apresentar características como: penca contendo 10 a 12 dedos (frutos, dedos de tamanho médio ou grande, diâmetro médio, quina presente, ausência de pintas pretas na casca, cor da polpa amarelo-clara ou média, textura firme, aroma e sabor de intensidade média, mediamente doce e vida útil de 7 a 10 dias em condição ambiente. O sabor, vida útil e aparência dos frutos de banana são considerados os mais importantes atributos na escolha ou compra da banana, segundo os consumidores entrevistados.Brazil has an approximate production of six million annual tons of banana (Musa spp., with a consumption close to 35 kg / inhabitant / year. The acceptance of the banana fruit is due, mainly, to its sensorial aspects, nutritional value and convenience. The identification of the customers' needs and desires consists of a critical activity of the marketing. The objective of this work was to research the consumer preferences of a local market (Cruz

  7. Carbon footprint of premium quality export bananas: case study in Ecuador, the world's largest exporter.

    Science.gov (United States)

    Iriarte, Alfredo; Almeida, Maria Gabriela; Villalobos, Pablo

    2014-02-15

    Nowadays, the new international market demands challenge the food producing countries to include the measurement of the environmental impact generated along the production process for their products. In order to comply with the environmentally responsible market requests the measurement of the greenhouse gas emissions of Ecuadorian agricultural goods has been promoted employing the carbon footprint concept. Ecuador is the largest exporter of bananas in the world. Within this context, this study is a first assessment of the carbon footprint of the Ecuadorian premium export banana (Musa AAA) using a considerable amount of field data. The system boundaries considered from agricultural production to delivery in a European destination port. The data collected over three years permitted identifying the hot spot stages. For the calculation, the CCaLC V3.0 software developed by the University of Manchester is used. The carbon footprint of the Ecuadorian export banana ranged from 0.45 to 1.04 kg CO2-equivalent/kg banana depending on the international overseas transport employed. The principal contributors to the carbon footprint are the on farm production and overseas transport stages. Mitigation and reduction strategies were suggested for the main emission sources in order to achieve sustainable banana production.

  8. Effect of banana on blood pressure of hypertensive individuals: a cross sectional study from Pokhara, Nepal

    Directory of Open Access Journals (Sweden)

    Gnanakshi. Dayanand

    2015-06-01

    Full Text Available Background Hypertension or high blood pressure is a critical condition which can strain the heart, injure blood vessels, leads to augment the risk of heart attack, stroke, kidney problems, and if untreated may cause death. Several herbal approaches have been made to treat hypertensive individuals. Banana is a well known tropical fruit with little known anti hypertensive properties. The objective of this research was to investigate changes in blood pressure after consuming banana among the hypertensive individuals. Methods A cross-sectional study design was used to conduct this research. Data was collected by questionnaire and personal interviewing. Two ripened bananas (Musa acuminata were provided to each subject for 20 days. Blood pressures of the participants were taken before and after the experimentation. Results Most of the subjects were in the age group >60 years followed by 30-40 years and 51-60 years. 57.1% of the respondents were female. Noticeable changes observed in the pre and post experimentation results. Systolic and diastolic blood pressure was significantly decreased after banana consumption. Conclusion Results of this research strongly supports that banana contains phytochemicals, thus its intake significantly reduces blood pressure among hypertensive individuals. However, more clinical studies in human are still required that may provide evidence of efficacy.

  9. Characterization of Radopholus similis resistance in Musa Spp. with emphasis on phytochemical analysis

    OpenAIRE

    Dhakshinamoorthy, Suganthagunthalam

    2011-01-01

    The burrowing nematode, Radopholus similis (Cobb, 1893) Thorne, 1949 is considered as the most damaging nematode species in commercial banana plantations. Nematicides have been intensively used to control plant-parasitic nematodes. However, many effective nematicides have been withdrawn from the market due to their adverse effects on the environment, non-target organisms and the accumulation of toxic residues in the food chain. The use of resistant cultivars is an efficient and economical alt...

  10. Genome-wide analysis of the AP2/ERF family in Musa species reveals divergence and neofunctionalisation during evolution.

    Science.gov (United States)

    Lakhwani, Deepika; Pandey, Ashutosh; Dhar, Yogeshwar Vikram; Bag, Sumit Kumar; Trivedi, Prabodh Kumar; Asif, Mehar Hasan

    2016-01-06

    AP2/ERF domain containing transcription factor super family is one of the important regulators in the plant kingdom. The involvement of AP2/ERF family members has been elucidated in various processes associated with plant growth, development as well as in response to hormones, biotic and abiotic stresses. In this study, we carried out genome-wide analysis to identify members of AP2/ERF family in Musa acuminata (A genome) and Musa balbisiana (B genome) and changes leading to neofunctionalisation of genes. Analysis identified 265 and 318 AP2/ERF encoding genes in M. acuminata and M. balbisiana respectively which were further classified into ERF, DREB, AP2, RAV and Soloist groups. Comparative analysis indicated that AP2/ERF family has undergone duplication, loss and divergence during evolution and speciation of the Musa A and B genomes. We identified nine genes which are up-regulated during fruit ripening and might be components of the regulatory machinery operating during ethylene-dependent ripening in banana. Tissue-specific expression analysis of the genes suggests that different regulatory mechanisms might be involved in peel and pulp ripening process through recruiting specific ERFs in these tissues. Analysis also suggests that MaRAV-6 and MaERF026 have structurally diverged from their M. balbisiana counterparts and have attained new functions during ripening.

  11. Efeito da banana da Terra verde e do albedo do maracujá sobre o perfil lipídico de ratos

    OpenAIRE

    BARBOSA, Eloiza Helena Carrijo

    2011-01-01

    Foram elaboradas farinhas de banana da terra verde (Musa AAB- Terra) e albedo de maracujá (Passiflora edulis f. flavicarpa) para o desenvolvimento do presente trabalho. Após determinação da composição química dessas farinhas realizou-se um ensaio biológico cujo objetivo foi avaliar os efeitos da farinha do albedo de maracujá (rica em fibra solúvel), da farinha de banana da terra verde (rica em amido resistente) sobre os níveis lipídicos de ratos com dieta hipercolesterolêmica. Para o ensai...

  12. Going Bananas over The Rainforest

    Science.gov (United States)

    Curriculum Review, 2005

    2005-01-01

    With a market of nearly $5 billion a year, the banana is the world's most popular fruit, and the most important food crop after rice, wheat, and maize. Banana businesses are economic pillars in many tropical countries, providing millions of jobs for rural residents. But, for much of its history, the banana industry was notorious for destructive…

  13. Social Interactions in Growing Bananas

    DEFF Research Database (Denmark)

    Van Den Broeck, Katleen; Dercon, Stefan

    This paper analyses whether agricultural information flows give rise to social learning effects in banana cultivation in Nyakatoke, a small Tanzanian village. Based on a village census, full information is available on socio-economic characteristics and banana production of farmer kinship members...... effects that produce positive externalities in banana output...

  14. Fruit physical characteristics, proximate, mineral and starch characterization of FHIA 19 and FHIA 20 plantain and FHIA 03 cooking banana hybrids

    OpenAIRE

    Annor, George Amponsah; Asamoah-Bonti, Prudence; Sakyi-Dawson, Esther

    2016-01-01

    Cooking banana and plantain (Musa spp. AAB and ABB groups), have over the years been affected by pest and diseases, resulting in various organizations developing disease resistant hybrids with superior agronomic potential. The characteristics of these improved varieties needs to be studied to ascertain their suitability for use in various food systems. This study aimed at evaluating the physical characteristics, proximate and minerals composition, and characterizing the starch of plantain and...

  15. A High-Throughput Regeneration and Transformation Platform for Production of Genetically Modified Banana.

    Science.gov (United States)

    Tripathi, Jaindra N; Oduor, Richard O; Tripathi, Leena

    2015-01-01

    Banana (Musa spp.) is an important staple food as well as cash crop in tropical and subtropical countries. Various bacterial, fungal, and viral diseases and pests such as nematodes are major constraints in its production and are currently destabilizing the banana production in sub-Saharan Africa. Genetic engineering is a complementary option used for incorporating useful traits in banana to bypass the long generation time, polyploidy, and sterility of most of the cultivated varieties. A robust transformation protocol for farmer preferred varieties is crucial for banana genomics and improvement. A robust and reproducible system for genetic transformation of banana using embryogenic cell suspensions (ECS) has been developed in this study. Two different types of explants (immature male flowers and multiple buds) were tested for their ability to develop ECS in several varieties of banana locally grown in Africa. ECS of banana varieties "Cavendish Williams" and "Gros Michel" were developed using multiple buds, whereas ECS of "Sukali Ndiizi" was developed using immature male flowers. Regeneration efficiency of ECS was about 20,000-50,000 plantlets per ml of settled cell volume (SCV) depending on variety. ECS of three different varieties were transformed through Agrobacterium-mediated transformation using gusA reporter gene and 20-70 independent transgenic events per ml SCV of ECS were regenerated on selective medium. The presence and integration of gusA gene in transgenic plants was confirmed by PCR, dot blot, and Southern blot analysis and expression by histochemical GUS assays. The robust transformation platform was successfully used to generate hundreds of transgenic lines with disease resistance. Such a platform will facilitate the transfer of technologies to national agricultural research systems (NARS) in Africa.

  16. Soil mycoflora of banana and cassava in peatland and alluvial soil in Bengkulu

    Directory of Open Access Journals (Sweden)

    SUCIATMIH

    2006-10-01

    Full Text Available In order to discover the diversity and population of soil fungi, a study was carried out at banana (Musa paradisiaca and cassava (Manihot utilissima plants where both those plants planted in peatland and alluvial soil. Soil fungi were isolated using serial dilution plate method and they were incubated at both room temperature (27-28oC and 45oC. This process was replicated two times for each sample. The result indicated that from 4 soil samples, 24 genera of fungi representing 4 Ascomycotina, 15 Deuteromycotina, and 5 Zygomycotina were detected. The highest soil fungi population was found in cassava planted in peat land and incubated at room temperature (8.5 105 cfu/ g dry soil, while the lower soil fungi population came from banana plant that was planted in peat land and incubated at 45oC (7.1 103 cfu/g dry soil.

  17. Anti-cancer potential of banana flower extract: An in vitro study

    Directory of Open Access Journals (Sweden)

    Varalakshmi Kilingar Nadumane

    2014-12-01

    Full Text Available Banana (Musa paradisiaca flower is rich in phytochemicals (vitamins, flavonoids, proteins and has antioxidant properties. The anti-cancer activity of banana flower extract has been evaluated on the cervical cancer cell line HeLa. The antiproliferative effects were evaluated by MTT assay. The extract was further purified by TLC and characterized by LC-MS method. The ethanol extract had significant cytotoxicity to HeLa cells with an IC50 of 20 µg/mL. By thin layer chromatography we could isolate three fractions out of which fraction 2 had exhibited maximum anti-proliferative effects with an IC50 value of <10 µg/mL. By LC-MS analysis, bioactive fraction was found to have an m/z value of 224.2 indicating it as a novel one.

  18. Caracterização de acessos de bananeira com base na concentração de compostos funcionais Characterization of banana accessions with base on functional compounds

    Directory of Open Access Journals (Sweden)

    Edson Perito Amorim

    2011-04-01

    Full Text Available O objetivo deste trabalho foi determinar a concentração de polifenóis totais, flavonóides, vitamina C e carotenóides totais em 61 acessos de banana pertencentes ao Banco Ativo de Germoplasma de bananeira da Embrapa Mandioca e Fruticultura. Foram detectadas diferenças significativas para todas as características analisadas por meio da análise de variância. A média para os teores de polifenóis totais entre os 61 acessos de banana foi de 38,06mg 100g-1, com variação de 12,51mg 100g-1 para o triplóide 'Torp' a 257,80mg 100g-1 para o tetraplóide 'Teparod'. A média para flavonóides foi de 2,09mg 100g-1, variando de 0,85mg 100g-1 ('Maravilha' AAAB a 6,63mg 100g-1 ('Teparod' ABBB. Para vitamina C, a média foi de 21,60mg 100g-1, variando de 8,60mg 100g-1 (tetraplóide 'Bucaneiro' a 76,82mg 100g-1 (tetraplóide 'Teparod'. A média do teor de carotenóides totais foi de 4,34mg g-1, variando de 1,18mg g-1 ('Champa Madras', triplóide ABB a 19,24mg g-1 ('Saney', triplóide AAB. Os diplóides Modok Gier e NBA-14 apresentaram, respectivamente, quatro e cinco vezes mais carotenóides totais que as cultivares do grupo Cavendish ('Nanica', 'Willians' e 'Lacatan'. Esses resultados permitem concluir sobre a possibilidade da obtenção de cultivares com altos níveis de compostos funcionais por meio de cruzamentos e seleção. Cultivares com este perfil têm potencial como alimento funcional, ajudando a prevenir doenças, por meio da neutralização de radicais livres.The objective of this research was to determine the concentration of polyphenols, flavonols, vitamin C and carotenoid and in 61 banana accessions from Musa germplasm collection from Embrapa Cassava and Fruits, Brazil. Was detected significant differences for all characteristic analyzed in ANOVA. The average for polyphenols among the 61 accessions was of 38.06mg 100g-1, with variation of 12.51mg 100g-1 for the triploid 'Torp' to 257.80mg 100g-1 for the tetraploid 'Teparod'. The average

  19. 不同品种香蕉内生菌分离及广谱拮抗菌的筛选%Endophytes Isolation and Broad-spectrum Antagonistic Bacterias Screening from Banana

    Institute of Scientific and Technical Information of China (English)

    王梦颖; 周登博; 井涛; 胡一凤; 高祝芬; 谢晴宜; 张锡炎; 戚春林

    2014-01-01

    In order to determine the main distribution of endophytes and their broad-spectrum antimicrobial activity, endophytes were obtained from healthy and diseased tissues of two disease-resistant and one disease susceptible banana cultivars. Endophytes were separated from roots, corms, pseudostems, leaves and store in the ultra-low on Luria-Bertani(LB), Yeast Extract with supplements(YE), and Potato Dextrose Agar(PDA)strain store medium. Then screened broad-spectrum antagonistic bacteria which against Fusarium oxysporum f. sp. Cubense, Curvularia lunata, Curvularia fallax, Corynespora cassiicola(Berk&Curt)Wei, Alternaria musae, Deightoniella troulosa, Colletotrichum musae, Pestalogiopsis sp., Btoryosphaeria dothidea. Taxonomy identification of 041, 04-1, 19-1, 03A-1 was conducted by evaluating morphologic characteristics and 16S rDNA gene sequences for phylogenetic analysis. After purification, total of 438 endophytes were obtained. The total of isolates showed that we obtained 240 strains bacteria, followed by 142 strains actinomycetes, and 56 strains fungi. The richest number of endophytes that isolated from diseased NanTian banana cultivars(128). Ten actinomyces and two bacterias were determined to possess antibiotic activity against Ten banana pathogens. Isolates 041 was the most effective and had 28.13±1.89 mm width of inhibition zone. Isolated 041, 04-1, 19-1, 034-1 were identified as Streptomyces misionensis.%旨在探究抗病品种与易感品种香蕉的健康株和病株内生菌与其中广谱拮抗菌的主要分布规律,并对广谱拮抗菌进行拮抗活性的测定。以样品根、球茎、假茎、叶为材料分离培养内生菌,在实验室条件下,筛选对供试的10种香蕉致病菌均有良好拮抗活性的菌株并测定它们的拮抗活性,对活性最强的菌株进行形态学、16S rDNA序列同源性分析。结果显示,分离得到内生菌438株,其中细菌240株,放线菌142株,真菌56株。抗病品种南天

  20. Expression profiles of a MhCTR1 gene in relation to banana fruit ripening.

    Science.gov (United States)

    Hu, Huei-Lin; Do, Yi-Yin; Huang, Pung-Ling

    2012-07-01

    The banana (Musa spp.) is a typical climacteric fruit of high economic importance. The development of bananas from maturing to ripening is characterized by increased ethylene production accompanied by a respiration burst. To elucidate the signal transduction pathway involved in the ethylene regulation of banana ripening, a gene homologous to Arabidopsis CTR1 (constitutive triple response 1) was isolated from Musa spp. (Hsien Jin Chiao, AAA group) and designated as MhCTR1. MhCTR1 spans 11.5 kilobases and consists of 15 exons and 14 introns with consensus GT-AG nucleotides situated at their boundaries. MhCTR1 encodes a polypeptide of 805 amino acid residues with a calculated molecular weight of 88.6 kDa. The deduced amino acid sequence of MhCTR1 demonstrates 55%, 56% and 55% homology to AtCTR1, RhCTR1, and LeCTR1, respectively. MhCTR1 is expressed mostly in the mature green pulp and root organs. During fruit development MhCTR1 expression increases just before ethylene production rises. Moreover, MhCTR1 expression was detected mainly in the pulps at ripening stage 3, and correlated with the onset of peel yellowing, while MhCTR1 was constitutively expressed in the peels. MhCTR1 expression could be induced by ethylene treatment (0.01 μL L(-1)), and MhCTR1 expression decreased in both peel and pulp 24 h after treatment. Overall, changes observed in MhCTR1 expression in the pulp closely related to the regulation of the banana ripening process.

  1. Genome-Wide Identification and Expression Analyses of Aquaporin Gene Family during Development and Abiotic Stress in Banana.

    Science.gov (United States)

    Hu, Wei; Hou, Xiaowan; Huang, Chao; Yan, Yan; Tie, Weiwei; Ding, Zehong; Wei, Yunxie; Liu, Juhua; Miao, Hongxia; Lu, Zhiwei; Li, Meiying; Xu, Biyu; Jin, Zhiqiang

    2015-08-20

    Aquaporins (AQPs) function to selectively control the flow of water and other small molecules through biological membranes, playing crucial roles in various biological processes. However, little information is available on the AQP gene family in bananas. In this study, we identified 47 banana AQP genes based on the banana genome sequence. Evolutionary analysis of AQPs from banana, Arabidopsis, poplar, and rice indicated that banana AQPs (MaAQPs) were clustered into four subfamilies. Conserved motif analysis showed that all banana AQPs contained the typical AQP-like or major intrinsic protein (MIP) domain. Gene structure analysis suggested the majority of MaAQPs had two to four introns with a highly specific number and length for each subfamily. Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening. Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress. Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

  2. Genome-Wide Identification and Expression Analyses of Aquaporin Gene Family during Development and Abiotic Stress in Banana

    Directory of Open Access Journals (Sweden)

    Wei Hu

    2015-08-01

    Full Text Available Aquaporins (AQPs function to selectively control the flow of water and other small molecules through biological membranes, playing crucial roles in various biological processes. However, little information is available on the AQP gene family in bananas. In this study, we identified 47 banana AQP genes based on the banana genome sequence. Evolutionary analysis of AQPs from banana, Arabidopsis, poplar, and rice indicated that banana AQPs (MaAQPs were clustered into four subfamilies. Conserved motif analysis showed that all banana AQPs contained the typical AQP-like or major intrinsic protein (MIP domain. Gene structure analysis suggested the majority of MaAQPs had two to four introns with a highly specific number and length for each subfamily. Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening. Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress. Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

  3. The "Blue Banana" Revisited

    NARCIS (Netherlands)

    Faludi, A.K.F.

    2015-01-01

    This essay is about the “Blue Banana”. Banana is the name given subsequently by others to a Dorsale européenne (European backbone) identified empirically by Roger Brunet. In a background study to the Communication of the European Commission ‘Europe 2000’, Klaus Kunzmann and Michael Wegener put forwa

  4. Field Plot Techniques for Black Sigatoka Evaluation in East African Highland Bananas

    Directory of Open Access Journals (Sweden)

    Okoro, JU.

    1997-01-01

    Full Text Available Number of plants per experimental unit and number of replications for the efficient and precise assessment of black sigatoka leaf spot disease caused by Mycosphaerella fijiensis in East African Highland bananas were determined. Two representative cultivars were used. Host response to black sigatoka infection was measured by recording the youngest leaf with necrotic spots. The number of plants per experimental unit was determined, using the methods of maximum curvature and comparison of variances, while the number of replications was estimated by Hatheway's method. The optimum experimental plot size was 3 plants (18 m2 for the beer banana cultivar 'Igitsiri', and 30 plants (180 m2 for the cooking banana cultivar 'Igisahira Gisanzwe', using the comparison of variances method. However, the optimum plot size was 15 plants (90 m2 for both cultivars using the method of maximum curvature. The latter statistical method was preferred because of the low precision of the estimates in the former method. Unreplicated trials with plots of 15 plants could be adequate to assess black sigatoka response in East African bananas if uniform disease pressure exists.

  5. Composition, digestibility and application in breadmaking of banana flour.

    Science.gov (United States)

    Juarez-Garcia, E; Agama-Acevedo, E; Sáyago-Ayerdi, S G; Rodríguez-Ambriz, S L; Bello-Pérez, L A

    2006-09-01

    Banana flour (BF) was obtained from unripe banana (Musa paradisiacal L.) and characterized in its chemical composition. Experimental bread was formulated with BF flour and the product was studied regarding chemical composition, available starch (AS), resistant starch (RS) and rate of starch digestion in vitro. The chemical composition of BF showed that total starch (73.36%) and dietary fiber (14.52%) were the highest constituents. Of the total starch, available starch was 56.29% and resistant starch 17.50%. BF bread had higher protein and total starch content than control bread, but the first had higher lipid amount. Appreciable differences were found in available, resistant starch and indigestible fraction between the bread studied, since BF bread showed higher resistant starch and indigestible fraction content. HI-based predicted glycemic index for the BF bread was 65.08%, which was significantly lower than control bread (81.88%), suggesting a "slow carbohydrate" feature for the BF-based goods. Results revealed BF as a potential ingredient for bakery products containing slowly digestible carbohydrates.

  6. Creation of Transgenic Bananas Expressing Human Lysozyme Gene for Panama Wilt Resistance

    Institute of Scientific and Technical Information of China (English)

    Xin-Wu PEI; Shi-Kai CHEN; Rui-Ming WEN; Shang YE; Jia-Qin HUANG; Yong-Qiang ZHANG; Bing-Shan WANG; Zhi-Xing WANG; Shi-Rong JIA

    2005-01-01

    Human lysozyme (HL) inhibits Fusarium oxysporum (FocR4) growth in vitro. To obtaintransgenic bananas (Musa spp.) that are resistant to Panama wilt (F. oxysporum), we introduced an HL genethat is driven by a constitutive cauliflower mosaic virus 35S promoter into the banana via Agrobacterium-mediated transformation. PCR confirmed that 51 transgenic plants were obtained. The development ofPanama wilt symptoms were examined after the plants had been grown in pots. The non-transgenic plantsdeveloped typical fusarium symptoms 60 d after FocR4 inoculation, whereas 24 of 51 transgenic plants remained healthy. The transgenic banana plants that showed resistance to FocR4 in the pots were then planted in a field that was heavily infected with FocR4 for further investigation. Eleven of 24 plants developed symptoms before bud emergence; another 11 plants showed symptoms after bud emergence and the remaining two plants, H-67 and H-144, remained healthy and were able to fruit. Northern blotting analysisdemonstrated that H-67 and H-144, bearing the strongest resistance to Panama wilt, had the highest level ofHL expression and that the expression of HL was well correlated with the FocR4 resistance of transgenicplants. We conclude that Agrobacterium-mediated transformation, with the assistance of particlebombardment, is a powerful approach for banana transformation and that a transgenic HL gene can causeresistance of the crop to FocR4 in the field.

  7. Efeito dos complexos enzimáticos clarificantes Clarex e CEC1-CTAA sobre a qualidade do suco de banana Effect of enzymatic clarifier complexes Clarex and CEC1-CTAA on the quality of banana juice

    Directory of Open Access Journals (Sweden)

    Marisa Helena Cardoso

    1999-05-01

    Full Text Available Neste trabalho foi observado o efeito dos complexos enzimáticos clarificantes Clarex e CEC1-CTAA, adicionados na proporção de 0,03% v/p sobre purê de banana (Musa cavendishii, em condições amenas de hidrólise (40ºC, 15 minutos visando determinar a qualidade, aqui representada pelos indicadores: rendimento; viscosidade; Brix; pH; composição centesimal; contagens de bolores e leveduras e de mesófilos, e propriedades sensoriais de cor, aroma, sabor e corpo dos sucos de banana clarificados. O suco clarificado com Clarex apresentou-se significativamente (p The effect of the clarifier enzymatic complexes Clarex and CEC1-CTAA, used in the proportion 0.03% v/w in industrialized banana (Musa cavendishii pulp, at the conditions of gentle hydrolysis 40 degree Celsius, 15 minutes, was observed to determine the quality here represented by indicators such as yield, viscosity, Brix, pH, centesimal composition, counts of moulds and yeasts and of mesophilics, and sensorial properties of color, aroma, flavor and body by both clarified banana juices. The juice clarified by Clarex was significantly (p < 0.01 more yellow, less grey, less opaque and less viscous than that obtained with CEC1-CTAA. There was no significant difference between the means of aroma of fresh banana and flavor in these juices. Furthermore, the values obtained for flavor for both juices were judged good (6.72 and 6.05 for the juices clarified with Clarex and CEC1- CTAA, respectively, because they were up the middle of the scale (from 0 to 10.

  8. Secagem de bananas prata e d'água por convecção forçada Drying of banana prata and banana d'água by forced convection

    Directory of Open Access Journals (Sweden)

    Soraia Vilela Borges

    2010-09-01

    Full Text Available Estudou-se a influência de variáveis como cultivar, formato (cilindro e disco, branqueamento e condições do ar aquecido (temperatura: 50 e 70 ºC e velocidade: 0,14 e 0,42 m/s sobre o comportamento de secagem convectiva de bananas com uso de modelagem matemática. As bananas foram desidratadas em secador de bandejas e pesada em intervalos pré-determinados. O modelo exponencial foi bem ajustado às curvas de secagem (R²: 0,98-0,99, mostrando que os fatores mais influentes sobre a taxa de secagem foram a temperatura, a velocidade do ar e o branqueamento. De acordo com as constantes cinéticas apresentadas pelo modelo recomenda-se a secagem de banana, em qualquer dos formatos estudados, nas seguintes condições: para banana-prata, uso de branqueamento e secagem a 50 ºC/0,42 m/s; e para banana-d'água, sem uso de branqueamento e secagem a 70 ºC/0,42 m/s.The influence of variables such as cultivar, shape (cylinder and disc, blanching, and heated air conditions (temperatures of 50 and 70 ºC and velocities of 0.14 and 0.42 m/s on convective drying behavior of bananas using mathematical modeling. The bananas were dehydrated in a tray dryer and were weighed in predetermined periods of time. The exponential model showed good agreement with the drying curves (R²: 0.98-0.99 indicating that the factors that influenced the drying rate the most were temperature, air velocity, and blanching. According to the kinetics constants obtained with the model, the drying of bananas is recommended, including all shapes investigated under the following conditions: banana prata, blanching and drying at 50 ºC/0.42 m/s; and banana d'água, no blanching and drying at 70 ºC/0.42 m/s.

  9. Acclimatation de vitroplants de bananier Musa sp. en culture hydroponique: impact de différentes concentrations en cuivre sur la croissance des vitroplants

    Directory of Open Access Journals (Sweden)

    Mazinga, MK.

    2013-01-01

    Full Text Available Acclimatation of Banana's Vitroplants (Musa sp. in Hydroponic Culture: Effects of Different Concentrations Copper on Growth of Vitroplants. Constraints due to copper and consequences of its accumulation in acclimatized banana in vitroplants have been studied in hydroponic culture. 0-100- 500-1000 ppm copper was added to the nutrient medium in hydroponic culture. At 1000 ppm, copper was accumulated in the roots but not in the aerial parts. Surprisingly, biomass of shoots and roots was augmented significantly at this concentration (with leaves as an exception. Plant height was reduced strongly even at 100 ppm CuSO4, although the copper content in shoots and leaves was very low.

  10. Viability of pollen grains of tetraploid banana

    Directory of Open Access Journals (Sweden)

    Taliane Leila Soares

    2016-01-01

    Full Text Available ABSTRACT Obtaining banana tetraploid cultivars from triploid strains results in total or partial reestablishment of fertility, allowing the occurrence of some fruits with seeds, a feature that is undesirable from a marketing perspective. The objective of this study was to assess the viability of pollen of 12 banana tetraploid hybrids (AAAB by means of in vitro germination and two histochemical tests (acetocarmine and 2,3,5-triphenyltetrazolium chloride. The pollen tube growth was evaluated by germinating grains in three culture media — M1: 0.03% Ca(NO3∙4H2O, 0.02% Mg(SO4∙7H2O, 0.01% KNO3, 0.01% H3BO3 and 15% sucrose; M2: 0.03% Ca(NO3∙4H2O, 0.01% KNO3, 0.01% H3BO3 and 10% sucrose; and M3: 0.015% H3BO3, 0.045% Ca3(PO42 and 25% sucrose. The acetocarmine staining indicated high viability (above 80%, except for the genotypes YB42-17 and Caprichosa, which were 76 and 70%, respectively. However, the in vitro germination rate was lower than 50% for all the genotypes, except for the hybrids YB42-17 (M1 and YB42-47 (M1. The medium M1 provided the greatest germination percentage and pollen tube growth. Among the genotypes assessed, YB42-47 presented the highest germination rate (61.5% and tube length (5.0 mm. On the other hand, the Vitória cultivar had the lowest germination percentage (8.2% in medium M1. Studies of meiosis can shed more light on the differences observed in the evaluated tetraploids, since meiotic irregularities can affect pollen viability.

  11. Heat shock transcription factors in banana: genome-wide characterization and expression profile analysis during development and stress response

    Science.gov (United States)

    Wei, Yunxie; Hu, Wei; Xia, Feiyu; Zeng, Hongqiu; Li, Xiaolin; Yan, Yu; He, Chaozu; Shi, Haitao

    2016-01-01

    Banana (Musa acuminata) is one of the most popular fresh fruits. However, the rapid spread of fungal pathogen Fusarium oxysporum f. sp. cubense (Foc) in tropical areas severely affected banana growth and production. Thus, it is very important to identify candidate genes involved in banana response to abiotic stress and pathogen infection, as well as the molecular mechanism and possible utilization for genetic breeding. Heat stress transcription factors (Hsfs) are widely known for their common involvement in various abiotic stresses and plant-pathogen interaction. However, no MaHsf has been identified in banana, as well as its possible role. In this study, genome-wide identification and further analyses of evolution, gene structure and conserved motifs showed closer relationship of them in every subgroup. The comprehensive expression profiles of MaHsfs revealed the tissue- and developmental stage-specific or dependent, as well as abiotic and biotic stress-responsive expressions of them. The common regulation of several MaHsfs by abiotic and biotic stress indicated the possible roles of them in plant stress responses. Taken together, this study extended our understanding of MaHsf gene family and identified some candidate MaHsfs with specific expression profiles, which may be used as potential candidates for genetic breeding in banana. PMID:27857174

  12. Micorriza arbuscular e matéria orgânica na aclimatização de mudas de bananeira, cultivar nanicão Arbuscular mycorrhizal and organic matter on the acclimatization of banana-tree seedlings, cv. Nanicão

    Directory of Open Access Journals (Sweden)

    Rosa Maria Barbosa Matos

    2002-12-01

    Full Text Available Com o objetivo de avaliar o efeito da inoculação de fungo micorrízico arbuscular na produção de mudas de qualidade, em sistema de pouco insumo, realizou-se um experimento com mudas micropropagadas de bananeira, cultivar Nanicão, em casa de vegetação do Centro Nacional de Pesquisa de Agrobiologia (Seropédica/RJ, em julho de 1998. O delineamento experimental empregado foi o de blocos ao acaso com quatro repetições, constituindo-se os tratamentos por três substratos (0, 10% e 20% de matéria orgânica, na presença e ausência de Glomus clarum. A partir dos 65 dias de aclimatização, as mudas desenvolvidas no substrato sem matéria orgânica, submetidas a inoculação com G. clarum, apresentaram efeito positivo significativo da inoculação na altura e no número de folhas. Aos 93 dias, as mudas infectadas, cultivadas no substrato com 0 e 10% de matéria orgânica, mostraram altura e diâmetro superior às não-infectadas. Na colheita, aos 95 dias, constatou-se aumento significativo da massa de folha, pseudocaule e raízes secas, bem como do conteúdo total de fósforo (P das mudas infectadas em relação às não-infectadas com G. clarum. A avaliação da razão de eficiência radicular mostrou que as mudas dos substratos sem matéria orgânica e com 10%, infectadas com G. clarum, apresentaram maior razão do que as não-infectadas. De maneira geral, a presença de matéria orgânica no substrato proporcionou efeito positivo no desenvolvimento das mudas de bananeira 'Nanicão'.With the objective of evaluating the inoculation effect of arbuscular mycorrhizal fungus on the development of good-quality banana seedlings, cv. Nanicão, under a low-input system, a greenhouse experiment was done at Seropédica, State of Rio de Janeiro, Brazil, in the National Center of Agrobiology Research, in 1998. The experimental design was a randomized block, with four repetitions. The treatments consisted of growing micropropagated seedlings in three

  13. Comparative Genomics of the Sigatoka Disease Complex on Banana Suggests a Link between Parallel Evolutionary Changes in Pseudocercospora fijiensis and Pseudocercospora eumusae and Increased Virulence on the Banana Host

    Science.gov (United States)

    Chang, Ti-Cheng; Crous, Pedro W.; Stergiopoulos, Ioannis

    2016-01-01

    The Sigatoka disease complex, caused by the closely-related Dothideomycete fungi Pseudocercospora musae (yellow sigatoka), Pseudocercospora eumusae (eumusae leaf spot), and Pseudocercospora fijiensis (black sigatoka), is currently the most devastating disease on banana worldwide. The three species emerged on bananas from a recent common ancestor and show clear differences in virulence, with P. eumusae and P. fijiensis considered the most aggressive. In order to understand the genomic modifications associated with shifts in the species virulence spectra after speciation, and to identify their pathogenic core that can be exploited in disease management programs, we have sequenced and analyzed the genomes of P. eumusae and P. musae and compared them with the available genome sequence of P. fijiensis. Comparative analysis of genome architectures revealed significant differences in genome size, mainly due to different rates of LTR retrotransposon proliferation. Still, gene counts remained relatively equal and in the range of other Dothideomycetes. Phylogenetic reconstruction based on a set of 46 conserved single-copy genes strongly supported an earlier evolutionary radiation of P. fijiensis from P. musae and P. eumusae. However, pairwise analyses of gene content indicated that the more virulent P. eumusae and P. fijiensis share complementary patterns of expansions and contractions in core gene families related to metabolism and enzymatic degradation of plant cell walls, suggesting that the evolution of virulence in these two pathogens has, to some extent, been facilitated by convergent changes in metabolic pathways associated with nutrient acquisition and assimilation. In spite of their common ancestry and shared host-specificity, the three species retain fairly dissimilar repertoires of effector proteins, suggesting that they likely evolved different strategies for manipulating the host immune system. Finally, 234 gene families, including seven putative effectors, were

  14. PMN Leukocytes and Fibroblasts Numbers on Wound Burn Healing on the Skin of White Rat after Administration of Ambonese Plantain Banana

    Directory of Open Access Journals (Sweden)

    Juniarti

    2012-04-01

    Full Text Available A study of ambonese plantain banana (Musa paradisiaca var sapientum Lamb treatment in burn wound healing on the skin of white rats (Rattus novergicus has been conducted. The wound healing of burn injuries was evaluated by counting the number of PMN leukocytes and fibroblasts at the 7th, 14th, and 21st days following the treatment. The study showed that the decrease in number of PMN leukocytes of subjects treated with ambonese plantain banana was relatively more significant compared to both negative and positive control (Bioplacenton ®. In contrast, an increasing number of fibroblasts was significantly demonstrated at the 14th and 21st days after treatment. In conclusion, ambonese plantain banana treatment in burn injuries will provide better results compared to both positive and negative controls.

  15. Efecto de la densidad de inóculo en la multiplicación y diferenciación de suspensiones celulares embriogénicas en el cultivar híbrido de banano FHIA-18 (Musa spp. AAAB

    Directory of Open Access Journals (Sweden)

    Luis A. Barranco Olivera

    2011-06-01

    Full Text Available La obtención de un sistema de regeneración eficiente por medio de la embriogénesis somática en las Musaceas, es hoy una gran herramienta ante los enormes problemas que presenta este género con el ataque de enfermedades como la sigatoka negra. El objetivo del trabajo es determinar las densidades celulares adecuadas para las etapas de multiplicación de suspensiones celulares embriogénicas y formación de los embriones somáticos en medios de cultivo líquidos. Como material vegetal se usaron brotes inmaduros de la inflorescencia masculina de Musa AAAB, cv. FHIA-18. Los resultados demostraron que es posible el establecimiento de suspensiones celulares homogéneas a partir de embriones somáticos en etapa globular, y obtener los mayores volúmenes de biomasa celular al multiplicar dichas suspensiones con una densidad del 3% del volumen de células sedimentadas. A partir del decimoquinto día en el medio de cultivo de formación de embriones comenzaron a formarse estructuras compuestas por proembriones y embriones somáticos en etapa globular; entre las densidades estudiadas los mejores resultados se obtuvieron con 100 mgMF en la cual se formaron 1 871 ES.l-1 con un peso de 248 mgMF.l-1 Palabras clave: embrión somático, Musaceas, densidad celular.

  16. 海南香蕉根颈象甲产卵选择性研究%Oviposition Preference of the Banana Weevil Cosmopolites sordidus in Hainan

    Institute of Scientific and Technical Information of China (English)

    卢辉; 王明军; 钟义海; 卢芙萍; 徐雪莲; 陈青

    2011-01-01

    The banana weevil, Cosmopolites sordidus (Germar), is a kind of important pest on bananas, causing serious damage to many banana gardens in Hainan. In order to clarify the oviposition characteristic of C. Sordidus on the banana in Hainan, and offer the guide for the field controlling technology, the oviposition preference of C. Sordidus adult on the different host plant and leaf sheath, and the oviposition rate with different feeding ways, were studied and analyzed experimentally in the laboratory. The results showed that the oviposition rate of C. Sordidus on Brazil banana (Musa AAA cavendish) and Thailand banana (Musa AAA Croup KlaiHom Thong) was 32.87% and 30.93% separately, which were significantly higher than that on Tai Chiao (Musa AAB Sikl), Plantain (Musa ABA), Huangdi banana (Musa AA Pisang Mas), and Fenjiao (Musa ABB Pisang Awak). The adult preferred to oviposit on the middle leaf sheath, particularly the second leaf sheath, with the highest selectivity coefficient of 45.78%, significantly higher than the others. Moreover, when rotten and fresh banana pesudostem were separately used for oviposition of adult female C. Sordidus, the weekly average oviposition rate was 4 and 2 respectively, indicating that C. Sordidus preferred to oviposite on rotten pesudostem.%为了研究香蕉根颈象甲在海南香蕉上的产卵特性,指导田间防控技术,室内研究了香蕉根颈象甲成虫对寄主植物、叶鞘部位的产卵选择性及不同饲养方式下的产卵率.结果表明,在巴西蕉和泰国蕉上,香蕉根颈象甲的产卵率分别为32.87%和30.93%,均显著高于台蕉、大蕉、皇帝蕉和粉蕉;香蕉根颈甲成虫嗜好在香蕉假茎中部叶鞘产卵,在第2层叶鞘的选择性最高,选择系数为45.78%,显著高于其它几层叶鞘的选择系数;分别提供腐烂和新鲜的香蕉假茎让香蕉根颈象甲产卵,周平均产卵量分别为4粒和2粒,可见,香蕉根颈象甲喜欢在腐烂的假茎上产卵.

  17. De Novo characterization of the banana root transcriptome and analysis of gene expression under Fusarium oxysporum f. sp. Cubense tropical race 4 infection

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    Wang Zhuo

    2012-11-01

    Full Text Available Abstract Background Bananas and plantains (Musa spp. are among the most important crops in the world due to their nutritional and export value. However, banana production has been devastated by fungal infestations caused by Fusarium oxysporum f. sp. cubense (Foc, which cannot be effectively prevented or controlled. Since there is very little known about the molecular mechanism of Foc infections; therefore, we aimed to investigate the transcriptional changes induced by Foc in banana roots. Results We generated a cDNA library from total RNA isolated from banana roots infected with Foc Tropical Race 4 (Foc TR 4 at days 0, 2, 4, and 6. We generated over 26 million high-quality reads from the cDNA library using deep sequencing and assembled 25,158 distinct gene sequences by de novo assembly and gap-filling. The average distinct gene sequence length was 1,439 base pairs. A total of 21,622 (85.94% unique sequences were annotated and 11,611 were assigned to specific metabolic pathways using the Kyoto Encyclopedia of Genes and Genomes database. We used digital gene expression (DGE profiling to investigate the transcriptional changes in the banana root upon Foc TR4 infection. The expression of genes in the Phenylalanine metabolism, phenylpropanoid biosynthesis and alpha-linolenic acid metabolism pathways was affected by Foc TR4 infection. Conclusion The combination of RNA-Seq and DGE analysis provides a powerful method for analyzing the banana root transcriptome and investigating the transcriptional changes during the response of banana genes to Foc TR4 infection. The assembled banana transcriptome provides an important resource for future investigations about the banana crop as well as the diseases that plague this valuable staple food.

  18. Banana Gold: Problem or Solution?

    Science.gov (United States)

    Joseph, Garnet

    1992-01-01

    Since 1955, the British banana industry has dominated the lives of the Caribs and other peoples in Dominica. Banana growing supplants other economic activities, including local food production; toxic chemicals and fertilizers pollute the land; community is dwindling; suicide is common; and child labor diminishes school attendance. (SV)

  19. Banana MaMADS Transcription Factors Are Necessary for Fruit Ripening and Molecular Tools to Promote Shelf-Life and Food Security.

    Science.gov (United States)

    Elitzur, Tomer; Yakir, Esther; Quansah, Lydia; Zhangjun, Fei; Vrebalov, Julia; Khayat, Eli; Giovannoni, James J; Friedman, Haya

    2016-05-01

    Genetic solutions to postharvest crop loss can reduce cost and energy inputs while increasing food security, especially for banana (Musa acuminata), which is a significant component of worldwide food commerce. We have functionally characterized two banana E class (SEPALLATA3 [SEP3]) MADS box genes, MaMADS1 and MaMADS2, homologous to the tomato (Solanum lycopersicum) RIN-MADS ripening gene. Transgenic banana plants repressing either gene (via antisense or RNA interference [RNAi]) were created and exhibited specific ripening delay and extended shelf-life phenotypes, including delayed color development and softening. The delay in fruit ripening is associated with a delay in climacteric respiration and reduced synthesis of the ripening hormone ethylene; in the most severe repressed lines, no ethylene was produced and ripening was most delayed. Unlike tomato rin mutants, banana fruits of all transgenic repression lines responded to exogenous ethylene by ripening normally, likely due to incomplete transgene repression and/or compensation by other MADS box genes. Our results show that, although MADS box ripening gene necessity is conserved across diverse taxa (monocots to dicots), unlike tomato, banana ripening requires at least two necessary members of the SEPALLATA MADS box gene group, and either can serve as a target for ripening control. The utility of such genes as tools for ripening control is especially relevant in important parthenocarpic crops such as the vegetatively propagated and widely consumed Cavendish banana, where breeding options for trait improvement are severely limited.

  20. Plantain and banana starches: granule structural characteristics explain the differences in their starch degradation patterns.

    Science.gov (United States)

    Soares, Claudinéia Aparecida; Peroni-Okita, Fernanda Helena Gonçalves; Cardoso, Mateus Borba; Shitakubo, Renata; Lajolo, Franco Maria; Cordenunsi, Beatriz Rosana

    2011-06-22

    Different banana cultivars were used to investigate the influences of starch granule structure and hydrolases on degradation. The highest degrees of starch degradation were observed in dessert bananas during ripening. Scanning electron microscopy images revealed smooth granule surface in the green stage in all cultivars, except for Mysore. The small and round granules were preferentially degraded in all of the cultivars. Terra demonstrated a higher degree of crystallinity and a short amylopectin chain length distribution, resulting in high starch content in the ripe stage. Amylose content and the crystallinity index were more strongly correlated than the distribution of amylopectin branch chain lengths in banana starches. α- and β-amylase activities were found in both forms, soluble in the pulp and associated with the starch granule. Starch-phosphorylase was not found in Mysore. On the basis of the profile of α-amylase in vitro digestion and the structural characteristics, it could be concluded that the starch of plantains has an arrangement of granules more resistant to enzymes than the starch of dessert bananas.

  1. Genome and transcriptome analysis of the fungal pathogen Fusarium oxysporum f. sp. cubense causing banana vascular wilt disease.

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    Lijia Guo

    Full Text Available BACKGROUND: The asexual fungus Fusarium oxysporum f. sp. cubense (Foc causing vascular wilt disease is one of the most devastating pathogens of banana (Musa spp.. To understand the molecular underpinning of pathogenicity in Foc, the genomes and transcriptomes of two Foc isolates were sequenced. METHODOLOGY/PRINCIPAL FINDINGS: Genome analysis revealed that the genome structures of race 1 and race 4 isolates were highly syntenic with those of F. oxysporum f. sp. lycopersici strain Fol4287. A large number of putative virulence associated genes were identified in both Foc genomes, including genes putatively involved in root attachment, cell degradation, detoxification of toxin, transport, secondary metabolites biosynthesis and signal transductions. Importantly, relative to the Foc race 1 isolate (Foc1, the Foc race 4 isolate (Foc4 has evolved with some expanded gene families of transporters and transcription factors for transport of toxins and nutrients that may facilitate its ability to adapt to host environments and contribute to pathogenicity to banana. Transcriptome analysis disclosed a significant difference in transcriptional responses between Foc1 and Foc4 at 48 h post inoculation to the banana 'Brazil' in comparison with the vegetative growth stage. Of particular note, more virulence-associated genes were up regulated in Foc4 than in Foc1. Several signaling pathways like the mitogen-activated protein kinase Fmk1 mediated invasion growth pathway, the FGA1-mediated G protein signaling pathway and a pathogenicity associated two-component system were activated in Foc4 rather than in Foc1. Together, these differences in gene content and transcription response between Foc1 and Foc4 might account for variation in their virulence during infection of the banana variety 'Brazil'. CONCLUSIONS/SIGNIFICANCE: Foc genome sequences will facilitate us to identify pathogenicity mechanism involved in the banana vascular wilt disease development. These will

  2. High-efficiency phenotyping for vitamin A in banana using artificial neural networks and colorimetric data

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    César Fernandes Aquino

    2016-01-01

    Full Text Available ABSTRACT Banana is one of the most consumed fruits in Brazil and an important source of minerals, vitamins and carbohydrates for human diet. The characterization of banana superior genotypes allows identifying those with nutritional quality for cultivation and to integrate genetic improvement programs. However, identification and quantification of the provitamin carotenoids are hampered by the instruments and reagents cost for chemical analyzes, and it may become unworkable if the number of samples to be analyzed is high. Thus, the objective was to verify the potential of indirect phenotyping of the vitamin A content in banana through artificial neural networks (ANNs using colorimetric data. Fifteen banana cultivars with four replications were evaluated, totaling 60 samples. For each sample, colorimetric data were obtained and the vitamin A content was estimated in the ripe banana pulp. For the prediction of the vitamin A content by colorimetric data, multilayer perceptron ANNs were used. Ten network architectures were tested with a single hidden layer. The network selected by the best fit (least mean square error had four neurons in the hidden layer, enabling high efficiency in prediction of vitamin A (r2 = 0.98. The colorimetric parameters a* and Hue angle were the most important in this study. High-scale indirect phenotyping of vitamin A by ANNs on banana pulp is possible and feasible.

  3. Corrosion Inhibition Studies of Mild Steel in Acid Medium Using Musa Acuminata Fruit Peel Extract

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    N. Gunavathy

    2012-01-01

    Full Text Available The inhibition effect of unripe fruit peel extract of Musa acuminata (Cultivar variety – Nendran (MNP on corrosion of mild steel in 1 N HCl has been investigated by weight loss and electrochemical impedance spectroscopy (EIS with various concentrations of the extract. The effect of temperature on the corrosion inhibition of mild steel in the temperature range of 30°C – 80°C was carried out. The results indicate that MNP extract act as an effective inhibitor in the acid environment and is of mixed type inhibitor having efficiency as high as 96% at 2% inhibitor concentration. The inhibition efficiency of MNP extract increases with the increase of concentration but decreases with the increase in temperature. The inhibitor achieves its inhibition by physical adsorption of nutrients of the peel extract on the surface of the mild steel. The experimental data revealed that the adsorption occurred according to the Langmuir and Temkin adsorption isotherm.

  4. Banana bunchy top virus in sub-Saharan Africa: investigations on virus distribution and diversity.

    Science.gov (United States)

    Kumar, P Lava; Hanna, R; Alabi, O J; Soko, M M; Oben, T T; Vangu, G H P; Naidu, R A

    2011-08-01

    Banana bunchy top virus (BBTV) was first reported from sub-Saharan Africa (SSA) from Democratic Republic of Congo (DRC) in the 1950s, has become invasive and spread into 11 countries in the region. To determine the potential threat of BBTV to the production of bananas and plantains (Musa spp.) in the sub-region, field surveys were conducted for the presence of banana bunchy top disease (BBTD) in the DRC, Angola, Cameroon, Gabon and Malawi. Using the DNA-S and DNA-R segments of the virus genome, the genetic diversity of BBTV isolates was also determined from these countries relative to virus isolates across the banana-growing regions around the world. The results established that BBTD is widely prevalent in all parts of DRC, Malawi, Angola and Gabon, in south and western part of Cameroon. Analysis of the nucleotide sequences of DNA-S and DNA-R indicate that BBTV isolates from these countries are genetically identical forming a unique clade within the 'South Pacific' phylogroup that includes isolates from Australia, Egypt, South Asia and South Pacific. These results imply that farmers' traditional practice of transferring vegetative propagules within and between countries, together with virus spread by the widely prevalent banana aphid vector, Pentalonia nigronervosa, could have contributed to the geographic expansion of BBTV in SSA. The results provided a baseline to explore sanitary measures and other 'clean' plant programs for sustainable management of BBTV and its vector in regions where the disease has already been established and prevent the spread of the virus to as yet unaffected regions in SSA.

  5. Nitrogen and potassium fertilization on ‘Caipira’ and ‘BRS Princesa’ bananas in the Ribeira Valley

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    Edson S. Nomura

    Full Text Available ABSTRACT ‘BRS Princesa’ (AAAB and ‘Caipira’ (AAA banana cultivars have similar sensorial features in comparison to the ‘Maçã’ banana. They are resistant to Panama disease, which allows them to grow in the Ribeira Valley, the largest banana plantation area in the São Paulo State. However, there is no information on how to fertilize crop under these edaphoclimatic conditions. This study aimed to evaluate the development and production of ‘Caipira’ and ‘BRS Princesa’ bananas, by applying four fertilization doses of N and K2O (no fertilization; 175 and 285 kg ha-1 year-1; 350 and 570 kg ha-1 year-1; 525 and 855 kg ha-1 year-1. The most adequate fertilizer recommendation for ‘Caipira’ and ‘BRS Princesa’ cultivars was 150% of the standard recommendation for banana (525 kg ha-1 year-1 of N and 855 kg ha-1 year-1 of K2O in both production cycles, promoting adequate growth and production, since most of the evaluated characteristics showed linear responses with the increase in the fertilization doses. ‘Caipira’ and ‘BRS Princesa’ require higher amounts of N and K than that recommended for the banana crop in the São Paulo State, in order to express their productive potential.

  6. Modulation of Banana Polyphenol Oxidase (Ppo Activity by Naturally Occurring Bioactive Compounds From Plant Extracts

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    Alamelumangai. M

    2015-01-01

    Full Text Available Polyphenol Oxidase (PPO (E.C number 1.14.18.1 was extracted from banana (Musa paradisiaca and partially purified by acetone precipitation. The enzyme was found to have high affinity towards its substrate, catechol. In this study, various plant extracts like Glycyrrhiza glabra, Rubia cordifolia, Hesperethusa crenulata and oil from the seeds of Hydnocarpus laurifolia were observed to modulate the activity of banana PPO. Method In this study, various plant extracts were observed to modulate the activity of banana PPO at two different concentrations (0.4 and 40 μg/ml concentrations Result Among these 4 plant extracts, Glycyrrhiza glabra and Rubia cordifolia were found to increase the activity of PPO up to 1.35- 2.7 fold at two different concentrations (4 and 40 μg/ml. Few other two samples like Chaulmogra oil (2 and 4 μl/ml and the Hesperethusa crenulata plant extract (0.4 and 40 μg/ml concentrations, when used at low concentrations decreased the enzyme activity (38 %. Conclusion The novelty of this study is to screen their naturally occurring bioactive compounds from the plant extracts and their inhibitory activity against PPO.

  7. The Influence of Variation in Time and HCl Concentration to the Glucose Produced from Kepok Banana

    Science.gov (United States)

    Widodo M, Rohman; Noviyanto, Denny; RM, Faisal

    2016-01-01

    Kepok banana (Musa paradisiaca) is a plant that has many advantagesfrom its fruit, stems, leaves, flowers and cob. However, we just tend to take benefit from the fruit. We grow and harvest the fruit without taking advantages from other parts. So they would be a waste or detrimental to animal nest if not used. The idea to take the benefit from the banana crop yields, especially cob is rarely explored. This study is an introduction to the use of banana weevil especially from the glucose it contains. This study uses current methods of hydrolysis using HCl as a catalyst with the concentration variation of 0.4 N, 0.6 N and 0.8 N and hydrolysis times variation of 20 minutes, 25 minutes and 30 minutes. The stages in the hydrolysis include preparation of materials, the process of hydrolysis and analysis of test results using Fehling and titrate with standard glucose solution. HCl is used as a catalyst because it is cheaper than the enzyme that has the same function. NaOH 60% is used for neutralizing the pH of the filtrate result of hydrolysis. From the results of analysis, known thatthe biggest yield of glucose is at concentration 0.8 N and at 30 minutes reaction, it contains 6.25 gram glucose / 20 gram dry sampel, and the convertion is 27.22% at 20 gram dry sampel.

  8. Use of competitive PCR to assay copy number of repetitive elements in banana.

    Science.gov (United States)

    Baurens, F C; Noyer, J L; Lanaud, C; Lagoda, P J

    1996-11-27

    Banana is one of the most important subtropical fruit crops. Genetic improvement by traditional breeding strategies is difficult and better knowledge of genomic structure is needed. Repeated sequences are powerful markers for genetic fingerprinting. The method proposed here to determine the copy number of nuclear repetitive elements is based on competitive reverse transcription-polymerase chain reaction and can also be used for quantifying cytosolic sequences. The reliability of this method was investigated on crude preparations of total DNA. Variations due to the heterogeneity of crude DNA extracts showed that a single locus reference is needed for accurate quantification. A mapped microsatellite locus was used to normalize copy number measurements. Copy number assay of repetitive elements using this method clearly distinguishes between the two banana subspecies investigated: Musa acuminata spp. banskii and M. acuminata spp. malaccensis. Two repetitive sequence families, pMaCIR1115 and pA9-26, were assayed that cover up to 1% of the M. acuminata genome. Their copy number varied up to six fold between the two subspecies. Furthermore, sequence quantification showed that mitochondrial genomes are present in crude leaf-extracted banana DNA at up to 40 copies per cell.

  9. Molecular Cloning and Characterization of Fruit Softening Related Gene Mannanase from Banana Fruit

    Institute of Scientific and Technical Information of China (English)

    ZHUANG Jun-ping; SU Jing; CHEN Wei-xin

    2006-01-01

    A 1 250 bp cDNA fragment encoding β-mannanase, named MaMAN, was cloned from banana (Musa spp cv. Baxi) fruit using degenerate primers designed with reference to the conserved nucleic acid sequences of known β-mannanase genes by RT-PCR. Sequence analysis showed that MaMAN cDNA encompassed a 1 085 bp open-reading frame (ORF), encoding a predicted polypeptide of 395 amino acids. Alignment of the deduced amino acid sequence of MaMAN and other putative β-mannanases showed that MaMAN has an identity of 86, 70, 69, 54, and 57%, respectively, to β-mannanases from tomato, lettuce, arabidopsis, carrot and oryza sativa. The catalytic residues: Asn203, Glu204, Glu318 and the active site residues: Arg86, His277, Tyr279, and Trp360, which were strictly conserved in the glycoside hydrolase family 5 to which all 3-mannanases belonged, were found in MaMAN. Semi-quantitative RT-PCR revealed that the level of MaMAN transcript in the pulp increased during banana fruit ripening, suggesting that MaMAN was likely to be involved highly in banana fruit softening.

  10. Callus cell, shoot and stem proliferation data from pineapple crown and banana inflorescence in vitro: Biochemical and antioxidant properties

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    ABM Sharif Hossain

    2017-02-01

    Full Text Available The data article contains the experimental data and images on the callus cell, shoot and stem proliferation from pineapple crown slice and banana inflorescence in vitro. Investigated data are related to the research article “Effects of benzylaminopurine and naphthalene acetic acid on proliferation and shoot growth of pineapple (Ananas comosus L. Merr in vitro” Alsaif et al. (2011 [1] and “Plantlet Production through Development of Competent Multiple Meristem Cultures from Male Inflorescence of Banana, Musa acuminta cv. ׳Pisang Mas׳(AA” Wirakarnain et al. (2008 [2]. In the experimental data 1, physiological, (shoot weight, number length and stem proliferation biochemical (total sugar and chlorophyll and nutritional ((K+ and NO3− data using BAP, MS medium and NAA growth regulators in pineapple have been explored. In the experimental data 2, physiological, (callus weight, shoot number and length biochemical (total sugar, chlorophyll, total phenol, DPPH and nutritional (K+ and NO3− data employing BAP +IAA, MS medium and NAA growth regulators in banana have been exhibited. Overall quantitative measurement was observed by Spectrophotometer. In the experimental data, BAP was shown the best effective hormone for the both pineapple and banana explants regeneration.

  11. Localization, Concentration, and Transmission Efficiency of Banana bunchy top virus in Four Asexual Lineages of Pentalonia aphids

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    Alberto Bressan

    2013-02-01

    Full Text Available Banana bunchy top virus (BBTV is the most destructive pathogenic virus of banana plants worldwide. The virus is transmitted in a circulative non-propagative manner by the banana aphid, Pentalonia nigronervosa Coquerel. In this work, we examined the localization, accumulation, and transmission efficiency of BBTV in four laboratory-established lineages of Pentalonia aphids derived from four different host plants: taro (Colocasia esculenta, heliconia (Heliconia spp., red ginger (Alpinia purpurata, and banana (Musa sp.. Mitochondrial sequencing identified three and one lineages as Pentalonia caladii van der Goot, a recently proposed species, and P. nigronervosa, respectively. Microsatellite analysis separated the aphid lineages into four distinct genotypes. The transmission of BBTV was tested using leaf disk and whole-plant assays, both of which showed that all four lineages are competent vectors of BBTV, although the P. caladii from heliconia transmitted BBTV to the leaf disks at a significantly lower rate than did P. nigronervosa. The concentration of BBTV in dissected guts, haemolymph, and salivary glands was quantified by real-time PCR. The BBTV titer reached similar concentrations in the guts, haemolymph, and salivary glands of aphids from all four lineages tested. Furthermore, immunofluorescence assays showed that BBTV antigens localized to the anterior midguts and the principal salivary glands, demonstrating a similar pattern of translocations across the four lineages. The results reported in this study showed for the first time that P. caladii is a competent vector of BBTV.

  12. Localization, concentration, and transmission efficiency of Banana bunchy top virus in four asexual lineages of Pentalonia aphids.

    Science.gov (United States)

    Watanabe, Shizu; Greenwell, April M; Bressan, Alberto

    2013-02-22

    Banana bunchy top virus (BBTV) is the most destructive pathogenic virus of banana plants worldwide. The virus is transmitted in a circulative non-propagative manner by the banana aphid, Pentalonia nigronervosa Coquerel. In this work, we examined the localization, accumulation, and transmission efficiency of BBTV in four laboratory-established lineages of Pentalonia aphids derived from four different host plants: taro (Colocasia esculenta), heliconia (Heliconia spp.), red ginger (Alpinia purpurata), and banana (Musa sp.). Mitochondrial sequencing identified three and one lineages as Pentalonia caladii van der Goot, a recently proposed species, and P. nigronervosa, respectively. Microsatellite analysis separated the aphid lineages into four distinct genotypes. The transmission of BBTV was tested using leaf disk and whole-plant assays, both of which showed that all four lineages are competent vectors of BBTV, although the P. caladii from heliconia transmitted BBTV to the leaf disks at a significantly lower rate than did P. nigronervosa. The concentration of BBTV in dissected guts, haemolymph, and salivary glands was quantified by real-time PCR. The BBTV titer reached similar concentrations in the guts, haemolymph, and salivary glands of aphids from all four lineages tested. Furthermore, immunofluorescence assays showed that BBTV antigens localized to the anterior midguts and the principal salivary glands, demonstrating a similar pattern of translocations across the four lineages. The results reported in this study showed for the first time that P. caladii is a competent vector of BBTV.

  13. 香蕉真菌性叶斑病病原种群结构季节性变化研究%Seasonal variation in population structure of banana mycotic leaf spot pathogen

    Institute of Scientific and Technical Information of China (English)

    林善海; 黄思良; 岑贞陆; 覃丽萍; 黎起秦; 付岗

    2011-01-01

    [目的]研究香蕉叶斑病病原真菌种群结构的季节性变化,为香蕉叶斑病的流行学研究和防治提供理论依据.[方法]2009年6~10月,对南宁市金陵镇第1年生香蕉的真菌性叶斑病病原随机抽样,检测单个病斑的病原菌,并分析每个时期病原菌的种群结构.[结果]南宁市金陵镇香蕉真菌性叶斑病病原至少有9种,主要病原为香蕉暗双孢菌(Cordana musae).叶斑病类型以单一病原侵染为主,其独立侵染的检出率随着季节的变化呈先升后降趋势,最高峰(8月15目)达88.8%;复合侵染的叶斑病类型以两种病原共同侵染居多,检出率的变化趋势与单一病原相反;除Alternaria musae和Pestalotiopsis menezesiana外,其他7种病原之间可共同侵染香蕉;Cordana musae的总检出率呈振幅式上升,最高检出率达92.8%.[结论]季节变化是影响香蕉真菌性叶斑病病原种群结构的一个主要环境因子.Cordana musae是田间香蕉真菌性叶斑病病原菌的优势种,建议香蕉叶斑病的防治主要针对由Cordana musae引起的灰纹病.%[Objective]The present work was carried out to study the seasonal variation in population structure of banana mycotic leaf spot pathogen in order to provide information for its control. [Method]The effects of fungal pathogens, causing mycotic leaf spot diseases, were investigated on annual growth of banana plantations at Jinling Township (Nanning) from June to October, 2009. Pathogens of leaf spot were examined and the population structure was analyzed during each period. [Result]At least nine fungal pathogens of mycotic leaf spots of banana were observed, amongst which, the Cordana musae was the major pathogen. The single-pathogen-induced banana leaf spots (SBLS) were predominant. The detection rate of SBLS was slightly higher and declined rapidly along with seasonal variations, with the peak rate (88.8%) detected on 15 August. The co-infected banana leaf spots (CBLS) with two

  14. 香蕉茎秆制造纸浆工艺研究%Process of Paper Pulp Production from Banana Stem

    Institute of Scientific and Technical Information of China (English)

    黎瑞珍

    2014-01-01

    The process of paper pulp production from banana (Musa ‘Paradisiaca’) stem and the effects of acetic acid con-centration on chemical degumming efficiency, the effects of hydrogen peroxide and ammonia concentration on bleaching effi-ciency for banana stem were analyzed. The results showed that the best concentration of the three reagents including hydrogen peroxide, ammonia and acetic acid was 6 mol/L, 5 mol/L and 6 mol/L, respectively. The paper pulps produced were the best when old banana stems were bleached and degummed under these concentrations.%对香蕉(Musa ‘Paradisiaca’)茎秆制造纸浆的工艺进行了研究,并探讨了乙酸溶液浓度对香蕉树原茎化学脱胶效果的影响及过氧化氢、氨水浓度对其漂白效果的影响,最终确定3种试剂的最佳浓度。结果表明,老的香蕉树原茎在加热的情况下选用6.0 mol/L的过氧化氢溶液、5.0 mol/L的氨水、6.0 mol/L的乙酸溶液进行漂白和脱胶,所得纸浆成品效果最好。

  15. Transcriptome and expression profile analysis of highly resistant and susceptible banana roots challenged with Fusarium oxysporum f. sp. cubense tropical race 4.

    Directory of Open Access Journals (Sweden)

    Ting-Ting Bai

    Full Text Available Banana wilt disease, caused by the fungal pathogen Fusarium oxysporum f. sp. cubense 4 (Foc4, is regarded as one of the most devastating diseases worldwide. Cavendish cultivar 'Yueyoukang 1' was shown to have significantly lower disease severity and incidence compared with susceptible cultivar 'Brazilian' in greenhouse and field trials. De novo sequencing technology was previously performed to investigate defense mechanism in middle resistant 'Nongke No 1' banana, but not in highly resistant cultivar 'Yueyoukang 1'. To gain more insights into the resistance mechanism in banana against Foc4, Illumina Solexa sequencing technology was utilized to perform transcriptome sequencing of 'Yueyoukang 1' and 'Brazilian' and characterize gene expression profile changes in the both two cultivars at days 0.5, 1, 3, 5 and 10 after infection with Foc4. The results showed that more massive transcriptional reprogramming occurs due to Foc4 treatment in 'Yueyoukang 1' than 'Brazilian', especially at the first three time points, which suggested that 'Yueyoukang 1' had much faster defense response against Foc4 infection than 'Brazilian'. Expression patterns of genes involved in 'Plant-pathogen interaction' and 'Plant hormone signal transduction' pathways were analyzed and compared between the two cultivars. Defense genes associated with CEBiP, BAK1, NB-LRR proteins, PR proteins, transcription factor and cell wall lignification were expressed stronger in 'Yueyoukang 1' than 'Brazilian', indicating that these genes play important roles in banana against Foc4 infection. However, genes related to hypersensitive reaction (HR and senescence were up-regulated in 'Brazilian' but down-regulated in 'Yueyoukang 1', which suggested that HR and senescence may contribute to Foc4 infection. In addition, the resistance mechanism in highly resistant 'Yueyoukang 1' was found to differ from that in middle resistant 'Nongke No 1' banana. These results explain the resistance in the

  16. Resfriamento de banana-prata com ar forçado Forced-air cooling of banana

    Directory of Open Access Journals (Sweden)

    BÁRBARA TERUEL

    2002-04-01

    Full Text Available Este trabalho apresenta as curvas de resfriamento de banana-prata (Musa balbisiana Colla e os valores do tempo de meio e sete oitavos de resfriamento, partindo do cálculo da Taxa Adimensional de Temperatura. Os frutos foram resfriados num sistema com ar forçado a 7ºC, umidade relativa de 87,6±3,8%, e velocidade do ar entre 1 e 0,2 m/s. Aplicou-se um delineamento experimental inteiramente casualizado, usando um esquema fatorial 2x2 (dois fluxos de ar (fatores e duas embalagens (níveis, para um nível de significância de 10%. Os fluxos de ar foram 1.933 a 1.160 m³/h, e as embalagens se diferenciaram pela porcentagem de área de abertura disponível para a ventilação (40 e 3,2%. Foi constatada uma diferença significativa no tempo de resfriamento, tanto quando aplicadas as duas taxas de ar como quando usadas as duas embalagens. O menor tempo de resfriamento foi atingido no tratamento que combinou a maior taxa de ar (1.933 m³/h com a embalagem de maior área de aberturas (40%. O maior tempo de resfriamento foi atingido no tratamento que combinou a menor taxa de ar (1160 m³/h com a embalagem de 3,2% de área efetiva de abertura. Os resultados obtidos demonstram que o tempo de resfriamento depende, em grande medida, da taxa de ar e do tipo de embalagem usada. O tempo de resfriamento variou em média entre 117 a 555 min, dependendo do tratamento aplicado. Não se constatou diferença significativa nas perdas de massa entre os diferentes tratamentos.This work presents the cooling curves for bananas Prata, (Musa balbisiana Colla, and determinates half-cooling and seven-eight cooling times and the cooling rate. Bananas were kept in a cold room with a forced-air system at 7ºC and RH = 87.6±3.8%. The experiment was conducted in a 2x2 factorial design, to test the effects of two flow rates (factors of air passing through the product, and two types of boxes (levels. The statistical analysis was performed at p<0.10. The air flow rates were 1933

  17. COMPONENTS OF CELL WALL, ENZYME ACTIVITY IN PEDICEL AND SUSCEPTIBILITY OF BANANAS TO FINGER DROP

    Directory of Open Access Journals (Sweden)

    GLORIA ANNABELL COBEÑA RUIZ

    2016-01-01

    Full Text Available ABSTRACT A major problem in post-harvest handling of bananas is the individual detachment of the fruit from the hands. This study aimed to establishing the relationship between carbohydrate concentration and enzyme activity in the pedicel region of three cultivars of bananas, resistant and susceptible to natural dropping, during post-harvest ripening, and the susceptibility of bananas to finger dropping. Cultivars ‘Terra’ (plantain, AAB group and ‘Prata’ (banana, AAB group triploids and the ‘Prata Graúda’ (banana, AAAB group tetraploid were used. The experiment was distributed in split plots, with three plots (cultivars and five subplots (peel color stages in a completely randomized design with three replications and three fruits per sample unit. ‘Terra’ showed resistance to dropping, even though the fruit were ripe, unlike ‘Prata Graúda’, which, starting from the fifth stage (yellow fruit with green tips, exhibited high susceptibility to dropping. At all ripening stages, the ‘Terra’ had the highest dry mass levels. In turn, the ‘Prata Graúda’ always maintained the lowest levels. The ‘Terra’ showed decreasing levels of pectins during ripening, whereas starch remained high even in the ripe fruit. About the enzymes studied, the results confirmed the increased resistance of the ‘Terra’ to dropping, allowing to conclude that polygalacturonase (PG and pectinametylesterase (PME are the key enzymes for the solubilization of the cell wall that accompanies ripening, thus playing a critical role in inducing natural dropping. The high susceptibility of the ’Prata Graúda’ to dropping is associated with the high activity of PG and PME and the low levels of dry mass; the greater resistance of the ‘Terra’ to dropping is related to higher accumulation of dry mass and starch in the pedicel.

  18. Proteome changes in banana fruit peel tissue in response to ethylene and high-temperature treatments.

    Science.gov (United States)

    Du, Lina; Song, Jun; Forney, Charles; Palmer, Leslie Campbell; Fillmore, Sherry; Zhang, ZhaoQi

    2016-01-01

    Banana (Musa AAA group) is one of the most consumed fruits in the world due to its flavor and nutritional value. As a typical climacteric fruit, banana responds to ethylene treatment, which induces rapid changes of color, flavor (aroma and taste), sweetness and nutritional composition. It has also been reported that ripening bananas at temperatures above 24 °C inhibits chlorophyll breakdown and color formation but increases the rate of senescence. To gain fundamental knowledge about the effects of high temperature and ethylene on banana ripening, a quantitative proteomic study employing multiplex peptide stable isotope dimethyl labeling was conducted. In this study, green (immature) untreated banana fruit were subjected to treatment with 10 μL L(-1) of ethylene for 24 h. After ethylene treatment, treated and untreated fruit were stored at 20 or 30 °C for 24 h. Fruit peel tissues were then sampled after 0 and 1 day of storage, and peel color and chlorophyll fluorescence were evaluated. Quantitative proteomic analysis was conducted on the fruit peels after 1 day of storage. In total, 413 common proteins were identified and quantified from two biological replicates. Among these proteins, 91 changed significantly in response to ethylene and high-temperature treatments. Cluster analysis on these 91 proteins identified 7 groups of changed proteins. Ethylene treatment and storage at 20 °C induced 40 proteins that are correlated with pathogen resistance, cell wall metabolism, ethylene biosynthesis, allergens and ribosomal proteins, and it repressed 36 proteins that are associated with fatty acid and lipid metabolism, redox-oxidative responses, and protein biosynthesis and modification. Ethylene treatment and storage at 30 °C induced 32 proteins, which were mainly similar to those in group 1 but also included 8 proteins in group 3 (identified as chitinase, cinnamyl alcohol dehydrogenase 1, cysteine synthase, villin-2, leucine-transfer RNA ligase, CP47

  19. Chemical and microbiological interactions between soils and roots in commercial banana plantations (Musa AAA, cv. Cavendish)

    NARCIS (Netherlands)

    Segura Mena, R.; Serrano, E.; Pocasangre, L.; Acuna, O.; Bertsch, F.; Stoorvogel, J.J.; Sandoval, J.A.

    2015-01-01

    A study was performed to determine the relationships between soil chemical and microbiological con-ditions and how they impact soil production. The study was carried out on six Costa Rican commercialbanana farms with high, medium and low productivity. In each of the farms sector with relatively good

  20. Genome-wide Expression Analysis and Metabolite Profiling Elucidate Transcriptional Regulation of Flavonoid Biosynthesis and Modulation under Abiotic Stresses in Banana

    Science.gov (United States)

    Pandey, Ashutosh; Alok, Anshu; Lakhwani, Deepika; Singh, Jagdeep; Asif, Mehar H.; Trivedi, Prabodh K.

    2016-01-01

    Flavonoid biosynthesis is largely regulated at the transcriptional level due to the modulated expression of genes related to the phenylpropanoid pathway in plants. Although accumulation of different flavonoids has been reported in banana, a staple fruit crop, no detailed information is available on regulation of the biosynthesis in this important plant. We carried out genome-wide analysis of banana (Musa acuminata, AAA genome) and identified 28 genes belonging to 9 gene families associated with flavonoid biosynthesis. Expression analysis suggested spatial and temporal regulation of the identified genes in different tissues of banana. Analysis revealed enhanced expression of genes related to flavonol and proanthocyanidin (PA) biosynthesis in peel and pulp at the early developmental stages of fruit. Genes involved in anthocyanin biosynthesis were highly expressed during banana fruit ripening. In general, higher accumulation of metabolites was observed in the peel as compared to pulp tissue. A correlation between expression of genes and metabolite content was observed at the early stage of fruit development. Furthermore, this study also suggests regulation of flavonoid biosynthesis, at transcriptional level, under light and dark exposures as well as methyl jasmonate (MJ) treatment in banana. PMID:27539368

  1. STUDIES ON VASCULAR INFECTION OF FUSARIUM OXYSPORUM F. SP. CUBENSE RACE 4 IN BANANA BY FIELD SURVEY AND GREEN FLUORESCENT PROTEIN REPORTER

    Directory of Open Access Journals (Sweden)

    Bo Liu

    2013-04-01

    Full Text Available Fusarium wilt of banana (Musa spp. caused by Fusarium oxysporum f. sp. cubense (Foc is one of the most serious banana fungal diseases in the world. Understanding the infection process of Foc is important for development of effective ways in disease control. In order to follow infection and colonization of this pathogen from root to rhizome and pseudostem tissues of banana, a highly pathogenic strain FJAT-3076 of Foc race 4 (Foc4 was transformed with gene encoding green fluorescent protein (GFP and the fungus carrying gfp (FJAT-3076-GFP was used to inoculate banana plants (Cavendish cv. B.F.. After inoculation for 3 to 10 d, it was observed that the conidia and their germ-tubes had penetrated into epidermis of young roots. The hyphae were found inside the root xylem 10 d after inoculation in the rhizome and pseudostem xylem after inoculation for 17 d. All plants infected by Foc died in 24 d after inoculation. It was also observed that Foc had spread all over the xylem and part of hyphae reached the pseudostem surface. Hyphal population was found the highest in the pseudostem, lower in root and least in rhizome. Field survey confirmed that Foc4 were mostly present in the base of pseudostem and less in the rhizome. Thus, effective prevention of the Foc hyphae movement from the rhizome up to the pseudostem might delay or control banana wilt disease.

  2. Genome-wide Expression Analysis and Metabolite Profiling Elucidate Transcriptional Regulation of Flavonoid Biosynthesis and Modulation under Abiotic Stresses in Banana.

    Science.gov (United States)

    Pandey, Ashutosh; Alok, Anshu; Lakhwani, Deepika; Singh, Jagdeep; Asif, Mehar H; Trivedi, Prabodh K

    2016-08-19

    Flavonoid biosynthesis is largely regulated at the transcriptional level due to the modulated expression of genes related to the phenylpropanoid pathway in plants. Although accumulation of different flavonoids has been reported in banana, a staple fruit crop, no detailed information is available on regulation of the biosynthesis in this important plant. We carried out genome-wide analysis of banana (Musa acuminata, AAA genome) and identified 28 genes belonging to 9 gene families associated with flavonoid biosynthesis. Expression analysis suggested spatial and temporal regulation of the identified genes in different tissues of banana. Analysis revealed enhanced expression of genes related to flavonol and proanthocyanidin (PA) biosynthesis in peel and pulp at the early developmental stages of fruit. Genes involved in anthocyanin biosynthesis were highly expressed during banana fruit ripening. In general, higher accumulation of metabolites was observed in the peel as compared to pulp tissue. A correlation between expression of genes and metabolite content was observed at the early stage of fruit development. Furthermore, this study also suggests regulation of flavonoid biosynthesis, at transcriptional level, under light and dark exposures as well as methyl jasmonate (MJ) treatment in banana.

  3. The Classification of Musa L.Based on mtDNA ccb256 Sequence%芭蕉属植物基于线粒体ccb256序列的分类研究

    Institute of Scientific and Technical Information of China (English)

    邓长娟; 王静毅; 陈友; 冯慧敏; 徐小雄; 武耀廷

    2011-01-01

    The phylogeny and relationship among 42 accessions were investigated by analyzing DNA sequence obtained from mtDNA cytochrome c biogenesis orf256 (ccb256). The phylogenetic tree was constructed following maximum parsimony (MP) method using MEGA4. The results indicated that Australimusa and Callimusa section grouped together; AA and other species from Eumusa clustered with Rhodochlamys section. BB species from Eumusa section were placed a outgroup which revealed its primitive position in evolution of Musa L. In addition, Ice cream (ABB) and M. Balbisiana formed one group which showed that M. Balbisiana may be male parent providing B genotype for Ice cream. Except for Improved lady finger (AAB), other cultivated accessions and M. Acuminata formed a group. It was inferred that M. Acuminata provided A genotype for these accessions as a paternal plant. M. Acumlnata.ssp.banksii, which was considered to be the original parent of cultivars, grouped with almost all the cultivated accessions. It had a close relationship with cultivars. The results also showed that ccb256 sequence had a moderate evolutional rate, which should assist in gaining a better understanding of the evolution of wild and cultivated bananas and of the relationships between diploid and triploid bananas.%利用PCR和PCR产物直接序列分析技术,获得了芭蕉属42份材料ccb256线粒体DNA片段的序列.采用MEGA4软件构建了芭蕉属植物的MP树.结果表明,Australimusa组和Callimusa组聚到一起,真蕉组的AA、其它种和观赏蕉组聚到一起,无法明显区分.真蕉组的BB单独聚到一起,且位于MP的基部,揭示了其在芭蕉属植物进化中占据着最原始的地位.另外,栽培蕉Ice cream(ABB)与M.balbisiana聚到一起,可能是由M.balbisiana为父本提供B型供体杂交而来,而除Improved lady finger(AAB)外,其它的栽培蕉均与M.acuminata的亚(变)种聚到一起,推测由M.acuminata为父本提供A型供体杂交而来.被认为是所有栽培蕉

  4. Cultivo de bananas em diferentes áreas na ilha de Tenerife Banana production under different conditions in Tenerife island

    Directory of Open Access Journals (Sweden)

    Erval Rafael Damatto Junior

    2009-06-01

    Full Text Available Objetivando caracterizar a produção e a qualidade de bananas produzidas em diferentes condições de cultivo na ilha de Tenerife, foram estudadas três regiões da ilha (Cueva del Polvo, Hoya Melleque e Canaria Forestal, onde se produzem bananas ao ar livre das cultivares Gruesa, Gran Enana e Laja. Nas áreas de Cueva del Polvo e Hoya Melleque, emprega-se o cultivo convencional e, na propriedade Canaria Forestal, pratica-se o orgânico. Os espaçamentos adotados foram de 1,67 x 5,0 m, com duas plantas por cova; 1,3 x 3,0 m, com uma planta por cova, e 2,0 x 5,0 m, com duas plantas por cova, respectivamente, para as propriedades em Cueva del Polvo, Hoya Melleque e Canaria Forestal. Diante dos dados observados, é possível verificar que as plantas da cv. Gran Enana apresentam maior altura e as da cv. Gruesa, maior espessura de pseudocaule. Também se pode inferir que, dentre as áreas e cultivares estudadas, não houve grande variabilidade nas características físicas dos frutos. A produtividade média encontrada foi de 99,8 t.ha-1, valor considerado adequado.Aiming to characterize the production and the banana quality produced in different plantation conditions of Tenerife Island, three regions of the island were studied (Cueva del Polvo, Hoya Melleque and Canaria Forestal where bananas of Gruesa, Gran Enana and Laja cultivars are produced in open-air conditions. In Cueva del Polvo and Hoya Melleque the production was carried out in conventional management, while in Canaria Forestal the plants were carried out under organic system. Plants spacing was 1.67 x 5.0 m, with two plants per hole; 1.3 x 3.0 m, with one plant per hole and; 2.0 x 5.0 m, with two plants per hole, respectivitly to Cueva del Polvo, Hoya Melleque and Canaria Forestal. Our data show that plants of Gran Enana are higher and Gruesa plants have the thickest pseudostem. Differences were not found regarding the physical fruit characteristics and the average yield was 99.8 t.ha-1.

  5. Study on Banana Cooperatives in Hainan Province

    OpenAIRE

    Huang, Huide; ZHANG, Wanzhen; Liu, Enping; ZHANG, Xizhu

    2013-01-01

    This paper gives an overview of the distribution, member scale, production and operation of banana cooperatives in Hainan Province, and points out the market risk and natural risk faced by the production of banana cooperatives in Hainan Province. In order to promote the banana cooperatives to form new agricultural management system integrating organization and intensification, this paper puts forth the production and operation recommendations, such as joint production of banana cooperatives, ...

  6. Effect of Musa spp. extract on eggs and larvae of gastrointestinal nematodes from infected sheep

    Directory of Open Access Journals (Sweden)

    Natalie Neuwirt

    2015-12-01

    Full Text Available Helminthes are listed as one of the main problems facing the development of goat and sheep production. Haemonchus contortus is the specie that causes greatest negative impact in ranching. Resistance to anti-parasitic drugs and demand for residue-free animal-derived food products has elevated the importance of herbal treatments. The aim of this study was to develop an extract of Musa spp. and assess by in vitro testing, the anthelmintic effect on eggs and larvae in the gastrointestinal nematodes in sheep. Stool samples from sheep naturally infected were used to obtain eggs and larvae and was then followed by a test of hatchability and a larval migration inhibition test. In vitro tests on the inhibition of larval hatchability at concentrations of 160 and 180 mg mL-1 of larval extracts and inhibition of migration at concentrations of 800 and 1000 mg mL-1 were observed. The results indicate that the use of banana leaf has an anthelmintic effect and that in vivo studies on the applicability of this technology to the field should be made to further understanding and bring more information to what has already been revealed in this study.

  7. Characteristics of Three Thioredoxin Genes and Their Role in Chilling Tolerance of Harvested Banana Fruit

    Science.gov (United States)

    Wu, Fuwang; Li, Qing; Yan, Huiling; Zhang, Dandan; Jiang, Guoxiang; Jiang, Yueming; Duan, Xuewu

    2016-01-01

    Thioredoxins (Trxs) are small proteins with a conserved redox active site WCGPC and are involved in a wide range of cellular redox processes. However, little information on the role of Trx in regulating low-temperature stress of harvested fruit is available. In this study, three full-length Trx cDNAs, designated MaTrx6, MaTrx9 and MaTrx12, were cloned from banana (Musa acuminata) fruit. Phylogenetic analysis and protein sequence alignments showed that MaTrx6 was grouped to h2 type with a typical active site of WCGPC, whereas MaTrx9 and MaTrx12 were assigned to atypical cys his-rich Trxs (ACHT) and h3 type with atypical active sites of GCAGC and WCSPC, respectively. Subcellular localization indicated that MaTrx6 and MaTrx12 were located in the plasma membrane and cytoplasm, respectively, whereas MaTrx9 showed a dual cytoplasmic and chloroplast localization. Application of ethylene induced chilling tolerance of harvested banana fruit, whereas 1-MCP, an inhibitor of ethylene perception, aggravated the development of chilling injury. RT-qPCR analysis showed that expression of MaTrx12 was up-regulated and down-regulated in ethylene- and 1-MCP-treated banana fruit at low temperature, respectively. Furthermore, heterologous expression of MaTrx12 in cytoplasmic Trx-deficient Saccharomyces cerevisiae strain increased the viability of the strain under H2O2. These results suggest that MaTrx12 plays an important role in the chilling tolerance of harvested banana fruit, possibly by regulating redox homeostasis. PMID:27618038

  8. Characteristics of Three Thioredoxin Genes and Their Role in Chilling Tolerance of Harvested Banana Fruit

    Directory of Open Access Journals (Sweden)

    Fuwang Wu

    2016-09-01

    Full Text Available Thioredoxins (Trxs are small proteins with a conserved redox active site WCGPC and are involved in a wide range of cellular redox processes. However, little information on the role of Trx in regulating low-temperature stress of harvested fruit is available. In this study, three full-length Trx cDNAs, designated MaTrx6, MaTrx9 and MaTrx12, were cloned from banana (Musa acuminata fruit. Phylogenetic analysis and protein sequence alignments showed that MaTrx6 was grouped to h2 type with a typical active site of WCGPC, whereas MaTrx9 and MaTrx12 were assigned to atypical cys his-rich Trxs (ACHT and h3 type with atypical active sites of GCAGC and WCSPC, respectively. Subcellular localization indicated that MaTrx6 and MaTrx12 were located in the plasma membrane and cytoplasm, respectively, whereas MaTrx9 showed a dual cytoplasmic and chloroplast localization. Application of ethylene induced chilling tolerance of harvested banana fruit, whereas 1-MCP, an inhibitor of ethylene perception, aggravated the development of chilling injury. RT-qPCR analysis showed that expression of MaTrx12 was up-regulated and down-regulated in ethylene- and 1-MCP-treated banana fruit at low temperature, respectively. Furthermore, heterologous expression of MaTrx12 in cytoplasmic Trx-deficient Saccharomyces cerevisiae strain increased the viability of the strain under H2O2. These results suggest that MaTrx12 plays an important role in the chilling tolerance of harvested banana fruit, possibly by regulating redox homeostasis.

  9. Study on Banana Cooperatives in Hainan Province

    Institute of Scientific and Technical Information of China (English)

    Huide; HUANG; Wanzhen; ZHANG; Enping; LIU; Xizhu; ZHANG

    2013-01-01

    This paper gives an overview of the distribution,member scale,production and operation of banana cooperatives in Hainan Province,and points out the market risk and natural risk faced by the production of banana cooperatives in Hainan Province. In order to promote the banana cooperatives to form new agricultural management system integrating organization and intensification,this paper puts forth the production and operation recommendations,such as joint production of banana cooperatives,timely planting of banana,brand management,and improvement of production and operation technical level.

  10. Respiratory Contribution of the Alternate Path during Various Stages of Ripening in Avocado and Banana Fruits.

    Science.gov (United States)

    Theologis, A; Laties, G G

    1978-08-01

    The respiration of fresh slices of preclimacteric avocado (Persea americana Mill. var. Hass) and banana (Musa cavendishii var. Valery) fruits is stimulated by cyanide and antimycin. The respiration is sensitive to m-chlorobenzhydroxamic acid in the presence of cyanide but much less so in the presence of antimycin. In the absence of cyanide the contribution of the cyanide-resistant pathway to the coupled preclimacteric respiration is zero. In uncoupled slices, by contrast, the alternate path is engaged and utilized fully in avocado, and extensively in banana. Midclimacteric and peak climacteric slices are also cyanide-resistant and, in the presence of cyanide, sensitive to m-chlorobenzhydroxamic acid. In the absence of uncoupler there is no contribution by the alternate path in either tissue. In uncoupled midclimacteric avocado slices the alternate path is fully engaged. Midclimacteric banana slices, however, do not respond to uncouplers, and the alternate path is not engaged. Avocado and banana slices at the climacteric peak neither respond to uncouplers nor utilize the alternate path in the presence or absence of uncoupler.The maximal capacities of the cytochrome and alternate paths, V(cyt) and V(alt), respectively, have been estimated in slices from preclimacteric and climacteric avocado fruit and found to remain unchanged. The total respiratory capacity in preclimacteric and climacteric slices exceeds the respiratory rise which attends fruit ripening. In banana V(alt) decreases slightly with ripening.The aging of thin preclimacteric avocado slices in moist air results in ripening with an accompanying climacteric rise. In this case the alternate path is fully engaged at the climacteric peak, and the respiration represents the total potential respiratory capacity present in preclimacteric tissue. The respiratory climacteric in intact avocado and banana fruits is cytochrome path-mediated, whereas the respiratory climacteric of ripened thin avocado slices comprises

  11. Accumulation of soluble sugars in peel at high temperature leads to stay-green ripe banana fruit.

    Science.gov (United States)

    Yang, Xiaotang; Pang, Xuequn; Xu, Lanying; Fang, Ruiqiu; Huang, Xuemei; Guan, Peijian; Lu, Wangjin; Zhang, Zhaoqi

    2009-01-01

    Bananas (Musa acuminata, AAA group) fail to develop a yellow peel and stay green when ripening at temperatures >24 degrees C. The identification of the mechanisms leading to the development of stay-green ripe bananas has practical value and is helpful in revealing pathways involved in the regulation of chlorophyll (Chl) degradation. In the present study, the Chl degradation pathway was characterized and the progress of ripening and senescence was assessed in banana peel at 30 degrees C versus 20 degrees C, by monitoring relevant gene expression and ripening and senescence parameters. A marked reduction in the expression levels of the genes for Chl b reductase, SGR (Stay-green protein), and pheophorbide a oxygenase was detected for the fruit ripening at 30 degrees C, when compared with fruit at 20 degrees C, indicating that Chl degradation was repressed at 30 degrees C at various steps along the Chl catabolic pathway. The repressed Chl degradation was not due to delayed ripening and senescence, since the fruit at 30 degrees C displayed faster onset of various ripening and senescence symptoms, suggesting that the stay-green ripe bananas are of similar phenotype to type C stay-green mutants. Faster accumulation of high levels of fructose and glucose in the peel at 30 degrees C prompted investigation of the roles of soluble sugars in Chl degradation. In vitro incubation of detached pieces of banana peel showed that the pieces of peel stayed green when incubated with 150 mM glucose or fructose, but turned completely yellow in the absence of sugars or with 150 mM mannitol, at either 20 degrees C or 30 degrees C. The results suggest that accumulation of sugars in the peel induced by a temperature of 30 degrees C may be a major factor regulating Chl degradation independently of fruit senescence.

  12. 搜索Banana Republic

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    <正>国内没有Banana Republic的专营店,但这并不意味着你买不到它家的货品,渠道有二:其一,是去淘宝上寻找,超级强悍的淘宝网,点Banana Republic关键词,你能找到许多售卖原单货甚至美国代购货的店铺,价格平实,唯一的缺点就是不能试衣,Banana Republic尺码偏大,在购买时尽量比平时穿的尺码小一号。其二,就是去遍布青岛大街小巷的外贸服装店购买,笔者曾在许多家碰到过Banana Repubkic的原单货,但这就需要讲究机缘了,而我们写此文的目的之一也是希望读者今后在遇见这个品牌的原单货后,当机立断拿下,毕竟,"香蕉共和国"的货品在国内还是难得一遇的!

  13. Atividade antiviral de Musa acuminata Colla, Musaceae Antiviral activity of Musa acuminata Colla, Musaceae

    Directory of Open Access Journals (Sweden)

    Fernanda Otaviano Martins

    2009-09-01

    Full Text Available O presente trabalho avalia a atividade antiviral de extratos e frações de Musa acuminata Colla, Musaceae, coletada em duas regiões do Estado do Rio de Janeiro (Petrópolis e Santo Antônio de Pádua. As inflorescências de M. acuminata apresentaram excelente atividade para os dois vírus avaliados: herpesvírus simples humano tipo 1 e herpesvírus simples humano tipo 2, ambos resistentes ao Aciclovir. Os resultados indicam que os extratos de M. acuminata testados podem constituir alvo potencial para uso em terapias antivirais.This study evaluates the antiviral activity of extracts and fractions of Musa acuminata Colla collected in two regions of Rio de Janeiro State (Petrópolis and Santo Antônio de Pádua. The inflorescences of M. acuminata showed excellent activity for the two virus evaluated: simple human herpesvirus type 1 and simple human herpesvirus type 2, both resistant to Acyclovir. The results indicate that the tested extracts of M. acuminata can be potential target for use in antiviral therapy.

  14. Development of a thematic collection of Musa spp accessions using SCAR markers for preventive breeding against Fusarium oxysporum f. sp cubense tropical race 4.

    Science.gov (United States)

    Silva, P R O; de Jesus, O N; Bragança, C A D; Haddad, F; Amorim, E P; Ferreira, C F

    2016-03-11

    Bananas are one of the most consumed fruits worldwide, but are affected by many pests and diseases. One of the most devastating diseases is Fusarium wilt, caused by Fusarium oxysporum f. sp cubense (Foc). Recently, Fusarium tropical race 4 (Foc TR4) has been causing irreparable damage, especially in Asia and Africa where it has devastated entire plantations, including areas with Cavendish, which is known to be resistant to Foc race 1. Although this race is not yet present in Brazil, results obtained by Embrapa in partnership with the University of Wageningen, The Netherlands, indicate that 100% of the cultivars used by Brazilian growers are susceptible to Foc TR 4. In our study, 276 banana accessions were screened with sequence characterized amplified region (SCAR) markers that have been linked to the resistance of Foc TR 4. Two SCAR primers were tested and the results revealed that SCAR ScaU1001 was efficient at discriminating accessions with possible resistance in 36.6% of the evaluated accessions. This is the first attempt to develop a thematic collection of possible Foc TR 4 resistant banana accessions in Brazil, which could be tested in Asian or African countries to validate marker-assisted selection (MAS), and for use in the preventive breeding of the crop to safeguard our banana plantations against Foc TR 4. We believe that this is an important step towards the prevention of this devastating disease, especially considering that our banana plantations are at risk.

  15. Translating the “Banana Genome” to Delineate Stress Resistance, Dwarfing, Parthenocarpy and Mechanisms of Fruit Ripening

    Science.gov (United States)

    Dash, Prasanta K.; Rai, Rhitu

    2016-01-01

    Evolutionary frozen, genetically sterile and globally iconic fruit “Banana” remained untouched by the green revolution and, as of today, researchers face intrinsic impediments for its varietal improvement. Recently, this wonder crop entered the genomics era with decoding of structural genome of double haploid Pahang (AA genome constitution) genotype of Musa acuminata. Its complex genome decoded by hybrid sequencing strategies revealed panoply of genes and transcription factors involved in the process of sucrose conversion that imparts sweetness to its fruit. Historically, banana has faced the wrath of pandemic bacterial, fungal, and viral diseases and multitude of abiotic stresses that has ruined the livelihood of small/marginal farmers’ and destroyed commercial plantations. Decoding structural genome of this climacteric fruit has given impetus to a deeper understanding of the repertoire of genes involved in disease resistance, understanding the mechanism of dwarfing to develop an ideal plant type, unraveling the process of parthenocarpy, and fruit ripening for better fruit quality. Further, injunction of comparative genomics will usher in integration of information from its decoded genome and other monocots into field applications in banana related but not limited to yield enhancement, food security, livelihood assurance, and energy sustainability. In this mini review, we discuss pre- and post-genomic discoveries and highlight accomplishments in structural genomics, genetic engineering and forward genetic accomplishments with an aim to target genes and transcription factors for translational research in banana. PMID:27833619

  16. Influence of ginger and banana starches on the mechanical and disintegration properties of chloroquine phosphate tab-lets

    Institute of Scientific and Technical Information of China (English)

    O.A.Odeku; M.A.Odeniyi; G.O.Ogunlowo

    2009-01-01

    Objective:The influence of two experimental starches -ginger starch obtained from Zingiber officinale and ba-nana starch from Musa sapientum -on the mechanical and disintegration properties of chloroquine tablets have been studied in comparison with the influence of official corn starch.Methods:Chloroquine tablets were for-mulated using various concentarions of the starches as binding agent.The mechanical properties of the tablets were assessed in terms of crushing strength and friability and the crushing strength-friability ratio (CSFR) while drug release properties were evaluated based on disintegration and the time of tablets.Results:The ranking for crushing strength and CSFR was corn >banana >ginger starch while the ranking was reverse for friability.The disintegration time increased with packing fraction and starch concentration in the rank order of formulations containing corn >banana >ginger starch.The CSFR/DT values increased with concentration of starch binder indicating an improved balance between binding and disintegrant properties of the starches.Sta-tistical analysis showed that there were significant (P <0.001)difference in the CSFR/DT for tablets contai-ning the various starch binders.Conclusion:The mechanical and disintegration properties of the experimental starches compared favorably with those of corn starch and ginger starch could be more useful when faster tablet disintegration is desired.

  17. Ex-Ante Economic Impact Assessment of Genetically Modified Banana Resistant to Xanthomonas Wilt in the Great Lakes Region of Africa.

    Directory of Open Access Journals (Sweden)

    John Herbert Ainembabazi

    Full Text Available Credible empirical evidence is scanty on the social implications of genetically modified (GM crops in Africa, especially on vegetatively propagated crops. Little is known about the future success of introducing GM technologies into staple crops such as bananas, which are widely produced and consumed in the Great Lakes Region of Africa (GLA. GM banana has a potential to control the destructive banana Xanthomonas wilt disease.To gain a better understanding of future adoption and consumption of GM banana in the GLA countries which are yet to permit the production of GM crops; specifically, to evaluate the potential economic impacts of GM cultivars resistant to banana Xanthomonas wilt disease.The paper uses data collected from farmers, traders, agricultural extension agents and key informants in the GLA.We analyze the perceptions of the respondents about the adoption and consumption of GM crop. Economic surplus model is used to determine future economic benefits and costs of producing GM banana.On the release of GM banana for commercialization, the expected initial adoption rate ranges from 21 to 70%, while the ceiling adoption rate is up to 100%. Investment in the development of GM banana is economically viable. However, aggregate benefits vary substantially across the target countries ranging from US$ 20 million to 953 million, highest in countries where disease incidence and production losses are high, ranging from 51 to 83% of production.The findings support investment in the development of GM banana resistant to Xanthomonas wilt disease. The main beneficiaries of this technology development are farmers and consumers, although the latter benefit more than the former from reduced prices. Designing a participatory breeding program involving farmers and consumers signifies the successful adoption and consumption of GM banana in the target countries.

  18. A taxonomic revision of Musa aurantiaca (Musaceae) in Southeast Asia

    Institute of Scientific and Technical Information of China (English)

    Markku H(A)KKINEN; Henry V(A)RE

    2008-01-01

    Since the initial description, the name Musa aurantiaca Baker (1893) has been unclear to most botanists. The aim of this study is to settle its true identity and to update the description. The plant is distributed in the regions of Upper Assam and Arunachal Pradesh, India, Northern Myanmar and Tibet, China where it occurs commonly but it is not mentioned in Chinese literature at all. In this paper, the authors also review the description and the literature history of M. aurantiaca from 1893 to the present. Musa aurantiaca Baker is typified here.

  19. Cloning and Expression Analysis of an XET cDNA in the Peel and Pulp of Banana Fruit Ripening and Softening

    Institute of Scientific and Technical Information of China (English)

    LUWang-Jin; RyoheiNAKANO; YasutakaKUBO; AkitsuguINABAt; JIANGYue-Ming

    2004-01-01

    Xyloglucan endotransglycosylase (XET) is thought to be involved in fruit softening throughdisassembly of xyloglucan, which is the predominant hemicellulose of cell wall. To study the relationshipbetween fruit softening and XET during banana (Musa acuminata Colla cv. Grand Nain) fruit ripening, a fulllength cDNA (1 095 bp) encoding an XET, MA-XET1, was isolated from ripening banana fruit using RT-PCRand RACE-PCR (rapid amplification of cDNA ends) methods. Sequence analysis showed that the cDNAcontains 5' untranslated region of 66 bp, 3' untranslated region of 189 bp and ORF of 840 bp, encoding apredicted polypeptide of 280 amino acids, including DE|DFEFL motif, which is a presumptive catalyticdomain conserved in XETs. DNA gel blot analysis demonstrated that MA-XET1 is encoded by a multi-copyfamily in the banana genome. RNA gel blot analysis revealed that the level of MA-XET1 transcript in thepulp was undetectable, increased and decreased slightly at the preclimacteric, climacteric and postclimactericstages, respectively. In the peel, accumulation of MA-XET1 transcript was low, increased dramatically andthen decreased rapidly, at preclimacteric, climacteric and postclimacteric stages, respectively. Treatmentof fruit with propylene, an analog of ethylene, decreased the firmness and enhanced the accumulation ofMA-XET1 transcript in the peel and pulp. These results suggest that MA-XET1 is involved in softening ofthe peel and pulp during banana fruit ripening and its expression is regulated by ethylene at transcriptionallevel.

  20. Progresses on Pathogens of Banana Fusarium Wilt:a Review%香蕉枯萎病病原菌的研究进展

    Institute of Scientific and Technical Information of China (English)

    李斌; 盛鸥; 李春雨; 魏岳荣; 左存武; 胡春华; 易干军; 罗充

    2013-01-01

    Fusarium wilt of banana (Musa spp.), caused by soil-borne fungus Fusarium oxysporum f. sp. cubense (Foc), is one of the most serious and devastating diseases threatening the sustainable development of banana industry in the world. This review summarized the methodologies on isolation, incubation and identification of the pathogens of banana fusarium wilt, and highlighted recent findings relevant to classification and evolutionary relationships among the Foc vegetative compatibility groups (VCGs), and recent advances in molecular makers for rapid diagnosis of the pathogens. The present review may provide a reference guide to better understanding banana fusarium wilt for the researchers in China.%香蕉枯萎病是香蕉生产中最为严重、具有毁灭性的病害之一,已成为限制世界香蕉产业可持续发展的重要难题。本文根据国内外近年来香蕉枯萎病的研究概况,综述了病原菌的分离、培养和鉴定等基本研究方法,以及病原菌的分类、进化关系和分子鉴定等研究进展,以期能为中国香蕉枯萎病研究工作者提供借鉴,尽早解决香蕉枯萎病难题。

  1. Análise do comércio de bananas em Lavras: Minas Gerais Analysis of banana trade in Lavras: Minas Gerais

    Directory of Open Access Journals (Sweden)

    Lair Victor Pereira

    2009-06-01

    Full Text Available A participação de Lavras na oferta de banana no mercado local é muito pequena, considerando-se que o Brasil é o segundo país maior produtor com 6,6 milhões de toneladas e Minas Gerais é o quarto entre os Estados produtores dessa fruta. Visando a quantificar a participação de Lavras e região na oferta de banana no mercado local, realizou-se esse trabalho em duas etapas: 2002/2003 e 2004/2005. A aplicação mensal de questionários nos principais estabelecimentos comerciais de hortifruti e feiras - livre de Lavras, permitiu conhecer o volume comercializado, procedência e perdas das principais cultivares de banana. Os resultados obtidos mostram que em 2002/2003 foram comercializados 945,24 t e em 2004/2005 foi de 1.001,98 t. Desse volume, 6,56% em 2002/2003 e 14,62% em 2004/2005 tiveram como origem Lavras. O consumo per capita anual manteve-se em torno de 11,8 kg nos dois períodos pesquisados. As bananas tipo 'Prata', foram as mais comercializadas nas duas etapas, 54,7% no primeiro período e 58,7% no segundo, sendo que 7,91% e 18,35% , respectivamente, tiveram como origem Lavras. O volume de banana 'Marmelo' e do tipo 'Nanicão', foram de 1,91% e 28,4%, respectivamente, sendo que 84,0% da 'Marmelo' e 3,43% da tipo 'Nanicão' na segunda etapa foram procedentes de Lavras. A banana 'Maçã' teve uma redução de 125,30 t para 107,47 t, correspondendo a 13,26%, sendo que a oferta dessa cultivar, originada de Lavras, manteve-se em 13,8%. As bananas 'Maçã' e 'Marmelo' apresentaram as menores perdas, 3,56% e 4,78% e as dos tipos 'Prata'e 'Nanicão'as maiores perdas, 9,39% e 10, 75%, respectivamente.The participation of Lavras in the banana production offered to the local commerce is still very low considering that Brazil is the second banana producer of the world, with a production around 6.6 ton/year and per-capita consumption of 24.4 kg/year. Minas Gerais ranks in the fifth place among the most important Brazilian state producers. This

  2. Differentiation Potential and Chromosome Number of Embryogenic Suspension Cells of Musa spp. in Subculture%香蕉胚性悬浮细胞分化能力及染色体数目变异研究

    Institute of Scientific and Technical Information of China (English)

    洪林; 劳世辉; 梁国鲁; 魏岳荣; 刘正富

    2012-01-01

    以野生阿宽蕉(Musa itinerans Cheesman,AA)、抗枯1号(Kangku 1,AAA)、大蕉(Musa Paradidiaca L.,ABB)3个香蕉品种为试材,研究其胚性悬浮细胞在长期继代培养后的分化能力和染色体数目的变异.结果表明,3个香蕉品种ECS在继代培养过程中,胚性悬浮细胞染色体数目均不稳定,染色体数目从6个到116个不等,各ECS均为整倍体和非整倍体组成的混倍体,随继代次数的增加,染色体数目变异的细胞比例随之增多,体细胞胚胎分化能力逐渐下降.野生阿宽蕉、抗枯1号和大蕉的ECS继代培养1.5 a后,细胞变异率分别为96.3%、93.0%和94.0%,而体胚分化能力分别为0.96×103、0.55×103、0.65×103个/mL PCV.试验为进一步研究香蕉ECS染色体变异机理提供新的线索.%A study on the changes of somatic embryogenesis potential and chromosome number of embryogenic suspension cells of Musa itinerans Cheesman, Kangku 1, Musa Paradidiaca L. in subculture was undertaken. The results showed that the chromosome number of the embryogenic suspension cells of three banana varieties ranged from 6 to 116 during subculture. After subculturing, ECS were mixoploidy which contained euploid and aneuploid. The percentage of cells with chromosome number changed in ECS was increased, however the overall differentiation of somatic embryos gradually fell down accompanyied by increased number of subculture. After subcultured 1.5 a later, the ECS mutation rates of Musa itinerans Cheesman, Kangku 1, Musa Paradidiaca L. was 96.3%, 93.0% and 94.0%, and somatic embryogenesis frequency of ECS was 0.96xl03, 0.55×103, 0.65x103 per mL packed cell volume respectively. The experimental results also provided new clue for further working on the mechanism of ECS variation.

  3. Preliminary Study of Embryogenic Callus Induction using Immature Banana Male Flowers and Stems of Tissue Culture Seedlings%香蕉雄花和组培苗假茎胚性愈伤组织诱导的初步研究

    Institute of Scientific and Technical Information of China (English)

    林志聪; 赵辉; 曾会才

    2012-01-01

    从外植体来源、生长调节剂种类及浓度等方面研究影响巴西蕉胚性愈伤组织诱导的因素。结果表明,以未成熟雄花为外植体,MS培养基+1 mg/L 2,4-D+1 mg/L IAA+1 mg/L NAA较适宜胚性愈伤组织诱导,26℃暗培养条件下,未成熟雄花的胚性愈伤组织诱导率为3%~4%。在MS培养基+0.7 mg/L 2,4-D+4 mg/L 6-BA中,以不同品种假茎为外植体的脱分化愈伤组织诱导率最高可达75%,但只有巴西(青杆)和海南本地米蕉可以诱导出胚性愈伤组织,诱导率分别为0.1%和0.5%.%An attempt was made to study factors which affect induction of embryogenic callus derived from the banana cultivar Brazil(Musa AAA Cavendish Subgroup cv.Brazil),including source of explants,plant hormones and concentration of plant hormones.Explants from immature male flowers were cultured on the MS at the presence of 2,4-D 4 mg/L + IAA 1 mg/L + NAA 1 mg/L and incubated in the dark at 26℃.The callus inducing rates were 3%~4% for immature male flowers.In the MS+2,4-D 0.7 mg/L+6-BA 4 mg/L,the different varieties of stems as explants for callus induction rate of up to 75%.However,only Banana Brazil(blue bar) and Hainan powder coke can induce embryogenic callus,induction rate was 0.1% and 0.5%.

  4. Controle da antracnose na pós-colheita de bananas-'prata' com produtos alternativos aos agrotóxicos convencionais Postharvest control of antracnose in 'prata' bananas with alternatives products to conventional pesticides

    Directory of Open Access Journals (Sweden)

    Ricardo José Zimmermann de Negreiros

    2013-03-01

    Full Text Available Produtos alternativos aos agrotóxicos convencionais foram avaliados no controle da antracnose causada por Colletotrichum musae em pós-colheita de bananas 'Prata' [Musa spp. (AAB]. Foram utilizados buquês com três frutos, com diâmetro médio de 32 mm a 36 mm, no estádio pré-climatérico, com coloração de casca totalmente verde. Os frutos foram pulverizados com uma suspensão de conídios de C. musae, na concentração de 2,5x10(5 conídios/mL e mantidos em câmara úmida a 25 ºC, por 24 horas. Após esse período, foram pulverizados com as caldas dos produtos alternativos extrato cítrico 'Biogermex', óleo de nim 'Organic Neem' e óleo de alho 'Probinatu', na concentração de 10,0 mL/L, óleo de pimenta-longa e óleo de cravo-da-índia na concentração de 5,0 mL/L e quitosana na concentração de 10,0 mg/mL, além do fungicida Tectoï SC (tiabendazol na concentração de 0,65 mL/L. Água destilada foi utilizada como tratamento-testemunha. Os frutos tratados com quitosana, óleo de nim e óleo de alho tiveram a severidade da doença reduzida. O óleo de alho foi o produto mais eficiente, com redução também da incidência da doença. A qualidade dos frutos não foi depreciada por nenhum dos tratamentos alternativos nas concentrações utilizadas.Alternatives products to conventional pesticides in the control of anthracnose caused by Colletotrichum musae after harvest of 'Prata' banana [Musa spp. (AAB] were evaluated. Fruits with an average diameter of 32-36 mm were selected at the pre-climacteric stage, with a peel color index of 1 (totally green peel. Hands were subdivided into clusters of three fruits. The fruits were sprayed with a conidial suspension of C. musae at a concentration of 2.5 x 10(5 conidia/mL and were kept in a moist chamber at 25°C for 24 hours. After this period, fruits were sprayed with the alternative products 'Biogermex' citric extract, 'Organic Neem' oil and 'Probinatu' garlic oil at a concentration of 10.0 m

  5. Effect of Musa sapientum stem extract on animal models of depression

    Directory of Open Access Journals (Sweden)

    Aditya J Reddy

    2016-01-01

    Full Text Available Background: Musa sapientum, the banana plant, has shown to possess antioxidant activity in previous studies. Oxidative stress has been linked to the pathogenesis of major depressive disorder (MDD with evidence of increased serum levels of oxidative stress biomarkers in MDD patients. Objective: The present study aimed to evaluate the antidepressant activity of M. sapientum stem extract (MSSE in experimental models in mice. Materials and Methods: Forced swim test (FST and tail suspension test (TST were carried out in five different groups (n = 6/group of mice. The vehicle, standard drug, and the three test groups were orally administered distilled water (10 mL/kg, fluoxetine (25 mg/kg, and incremental doses of 25, 50, and 100 mg of MSSE, respectively, 45 min prior to the experiment. Results: On FST, the duration of immobility in control group, which was 161.5 ± 6.78 (in seconds, mean ± standard error of mean [SEM], decreased to 149.33 ± 2.70 (25 mg/kg MSSE, 120.17 ± 8.35 (50 mg/kg MSSE, and 45.17 ± 4.11 (100 mg/kg MSSE in the treated groups. On TST, the duration of immobility in control group, which was 173.83 ± 12.65 (mean ± SEM, decreased to 163.17 ± 6.91 (25 mg/kg MSSE, 139.0 ± 5.9 (50 mg/kg MSSE, and 124.0 ± 4.42 (100 mg/kg MSSE in the treated groups. The difference in the duration of immobility was statistically significant at middle and higher doses, i.e. 50 and 100 mg/kg MSSE (P < 0.05 respectively, when compared with the control group in both the tests. Conclusion: A significant antidepressant-like activity was found in MSSE, which could be a potential natural compound for use in depression.

  6. Effect of Musa sapientum Stem Extract on Animal Models of Depression

    Science.gov (United States)

    Reddy, Aditya J.; Handu, Shailendra S.; Dubey, Ashok Kumar; Mediratta, Pramod Kumari; Shukla, Rimi; Ahmed, Qazi Mushtaq

    2016-01-01

    Background: Musa sapientum, the banana plant, has shown to possess antioxidant activity in previous studies. Oxidative stress has been linked to the pathogenesis of major depressive disorder (MDD) with evidence of increased serum levels of oxidative stress biomarkers in MDD patients. Objective: The present study aimed to evaluate the antidepressant activity of M. sapientum stem extract (MSSE) in experimental models in mice. Materials and Methods: Forced swim test (FST) and tail suspension test (TST) were carried out in five different groups (n = 6/group) of mice. The vehicle, standard drug, and the three test groups were orally administered distilled water (10 mL/kg), fluoxetine (25 mg/kg), and incremental doses of 25, 50, and 100 mg of MSSE, respectively, 45 min prior to the experiment. Results: On FST, the duration of immobility in control group, which was 161.5 ± 6.78 (in seconds, mean ± standard error of mean [SEM]), decreased to 149.33 ± 2.70 (25 mg/kg MSSE), 120.17 ± 8.35 (50 mg/kg MSSE), and 45.17 ± 4.11 (100 mg/kg MSSE) in the treated groups. On TST, the duration of immobility in control group, which was 173.83 ± 12.65 (mean ± SEM), decreased to 163.17 ± 6.91 (25 mg/kg MSSE), 139.0 ± 5.9 (50 mg/kg MSSE), and 124.0 ± 4.42 (100 mg/kg MSSE) in the treated groups. The difference in the duration of immobility was statistically significant at middle and higher doses, i.e. 50 and 100 mg/kg MSSE (P Forced swim test; TST: Tail suspension test; GSH: Glutathione, MDA: Malondialdehyde; SOD: Superoxide dismutase PMID:27695263

  7. Energia alternativa de biomassa: bioetanol a partir da casca e da polpa de banana Alternative energy from biomass: bioethanol from banana pulp and peels

    Directory of Open Access Journals (Sweden)

    Ozair Souza

    2012-08-01

    Full Text Available A conversão de biomassas agroindustriais em bioetanol com consequente valorização de rejeitos e resíduos, tem sido objeto de estudos de várias pesquisas realizadas no Brasil e no mundo. Neste trabalho foi avaliada a potencialidade do uso da polpa e da casca da banana (Musa cavendishii , tanto in natura como previamente hidrolisada por ácido e enzimas, como substrato da fermentação alcoólica. Os rendimentos médios em bioetanol (em base úmida de biomassa obtidos com a polpa (0,48 ± 0,05 g g-1 e com a casca (0,34 ± 0,11 g g-1, ambos in natura, possibilitaram a eficiência do processo de conversão, da ordem de 95% do rendimento teórico. A produtividade máxima alcançada em bioetanol foi de 3,0 ± 0,7 g L-1 h-1 com o uso da polpa e de 1,32 ± 0,03 g L-1 h-1 com a casca. Nas condições operacionais avaliadas o pretratamento dos resíduos com ácido sulfúrico não é recomendado para a produção de bioetanol.The conversion of agroindustrial biomasses in bioethanol with consequent enrichment of wastes has been the object of various research projects conducted in Brazil and around the world. This study evaluated the potential of the Musa cavendishii banana pulp and peels using in natural state and also waste previously hydrolyzed by acid and enzimes, as substrate of alcoholic fermentation. The mean bioethanol yields (on wet biomass base, obtained with the pulp (0.48 ± 0.05 g g-1 and with the peels (0.34 ± 0.11 g g-1, both in natural state, enabled a conversion process efficiency to the order of 95% of theoretical yield. Maximum value reached in bioethanol was 3.0 ± 0.7 g L-1 h-1 with pulp and 1.32 ± 0.03 g L-1 h-1 with peels. Under the evaluated operating conditions, the pre-treatment of wastes with sulfuric acid is not recommended for bioethanol production.

  8. Distribuição da sacarose-fosfato sintase e sacarose sintase em bananas durante o amadurecimento

    Directory of Open Access Journals (Sweden)

    BASSINELLO Priscila Z.

    1999-01-01

    Full Text Available A hidrólise do amido e a síntese de açúcares durante o amadurecimento da banana são transformações bioquímicas importantes, havendo evidências de que ocorrem de forma homogênea no fruto. Para confirmar este fato, amostras de banana nanicão (Musa spp. colhidas aos 110 dias pós-antese, foram coletadas no decorrer do amadurecimento e foram determinados os teores de amido, hexoses e sacarose e a atividade das enzimas sacarose-fosfato sintase (SPS e sacarose sintase (SS em diferentes partes do fruto. Observou-se que na banana verde, existe mais amido na porção periférica (18% do que na central (13%. Porém, a sua velocidade de degradação durante o amadurecimento é a mesma, o que resulta em teores diferenciados de amido residual na banana madura. Também o aparecimento e acúmulo de sacarose foi simultâneo nas duas regiões e coincidente com os valores máximos de atividade da SPS. Utilizando-se de técnica de identificação por anticorpos específicos para SS e SPS em tecidos verde e maduro, observou-se uma distribuição homogênea das enzimas e aparente correlação entre a cor desenvolvida e a variação de atividade.

  9. 33 CFR 117.263 - Banana River.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Banana River. 117.263 Section 117.263 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.263 Banana River. (a) The draw of the Mathers...

  10. Banana orchard inventory using IRS LISS sensors

    Science.gov (United States)

    Nishant, Nilay; Upadhayay, Gargi; Vyas, S. P.; Manjunath, K. R.

    2016-04-01

    Banana is one of the major crops of India with increasing export potential. It is important to estimate the production and acreage of the crop. Thus, the present study was carried out to evolve a suitable methodology for estimating banana acreage. Area estimation methodology was devised around the fact that unlike other crops, the time of plantation of banana is different for different farmers as per their local practices or conditions. Thus in order to capture the peak signatures, biowindow of 6 months was considered, its NDVI pattern studied and the optimum two months were considered when banana could be distinguished from other competing crops. The final area of banana for the particular growing cycle was computed by integrating the areas of these two months using LISS III data with spatial resolution of 23m. Estimated banana acreage in the three districts were 11857Ha, 15202ha and 11373Ha for Bharuch, Anand and Vadodara respectively with corresponding accuracy of 91.8%, 90% and 88.16%. Study further compared the use of LISS IV data of 5.8m spatial resolution for estimation of banana using object based as well as per-pixel classification and the results were compared with statistical reports for both the approaches. In the current paper we depict the various methodologies to accurately estimate the banana acreage.

  11. Quantitative Proteomic Analysis Reveals that Antioxidation Mechanisms Contribute to Cold Tolerance in Plantain (Musa paradisiaca L.;ABB Group) Seedlings

    Institute of Scientific and Technical Information of China (English)

    Qiaosong Yang; Junhua Wu; Chunyu Li; Yuerong Wei; Ou Sheng; Chunhua Hu; Ruibin Kuang

    2012-01-01

    Banana and its close relative,plantain are globally important crops and there is of considerable interest in optimizing their cultivation.Plantain has superior cold tolerance compared to banana and a thorough understanding of the molecular mechanisms and responses of plantain to cold stress has great potential value for developing cold tolerant banana cultivars.In this study,we used iTRAQ-based comparative proteomic analysis to investigate the temporal responses of plantain to cold stress.Plantain seedlings were exposed for 0,6 and 24 h of cold stress at 8℃ and subsequently allowed to recover for 24 h at 28℃.A total of 3,477 plantain proteins were identified,of which 809 showed differential expression from the three treatments.The majority of differentially expressed proteins were predicted to be involved in oxidation-reduction,including oxylipin biosynthesis,while others were associated with photosynthesis,photorespiration and several primary metabolic processes,such as carbohydrate metabolic process and fatty acid beta-oxidation.Western blot analysis and enzyme activity assays were performed on 7 differentially expressed,cold-response candidate plantain proteins in order to validate the proteomics data.Similar analyses of the 7 candidate proteins were performed in cold-sensitive banana to examine possible functional conservation and to compare the results to equivalent responses between the two species.Consistent results were achieved by Western blot and enzyme activity assays,demonstrating that the quantitative proteomics data collected in this study are reliable.Our results suggest that an increase of antioxidant capacity through adapted ROS scavenging capability,reduced production of ROS and decreased lipid peroxidation contribute to molecular mechanisms for the higher cold tolerance in plantain.To the best of our knowledge,this is the first report of a global investigation on molecular responses of plantain to cold stress by proteomic analysis.

  12. Quantitative proteomic analysis reveals that antioxidation mechanisms contribute to cold tolerance in plantain (Musa paradisiaca L.; ABB Group) seedlings.

    Science.gov (United States)

    Yang, Qiao-Song; Wu, Jun-Hua; Li, Chun-Yu; Wei, Yue-Rong; Sheng, Ou; Hu, Chun-Hua; Kuang, Rui-Bin; Huang, Yong-Hong; Peng, Xin-Xiang; McCardle, James A; Chen, Wei; Yang, Yong; Rose, Jocelyn K C; Zhang, Sheng; Yi, Gan-Jun

    2012-12-01

    Banana and its close relative, plantain are globally important crops and there is considerable interest in optimizing their cultivation. Plantain has superior cold tolerance compared with banana and a thorough understanding of the molecular mechanisms and responses of plantain to cold stress has great potential value for developing cold tolerant banana cultivars. In this study, we used iTRAQ-based comparative proteomic analysis to investigate the temporal responses of plantain to cold stress. Plantain seedlings were exposed for 0, 6, and 24 h of cold stress at 8 °C and subsequently allowed to recover for 24 h at 28 °C. A total of 3477 plantain proteins were identified, of which 809 showed differential expression from the three treatments. The majority of differentially expressed proteins were predicted to be involved in oxidation-reduction, including oxylipin biosynthesis, whereas others were associated with photosynthesis, photorespiration, and several primary metabolic processes, such as carbohydrate metabolic process and fatty acid beta-oxidation. Western blot analysis and enzyme activity assays were performed on seven differentially expressed, cold-response candidate plantain proteins to validate the proteomics data. Similar analyses of the seven candidate proteins were performed in cold-sensitive banana to examine possible functional conservation, and to compare the results to equivalent responses between the two species. Consistent results were achieved by Western blot and enzyme activity assays, demonstrating that the quantitative proteomics data collected in this study are reliable. Our results suggest that an increase of antioxidant capacity through adapted ROS scavenging capability, reduced production of ROS, and decreased lipid peroxidation contribute to molecular mechanisms for the increased cold tolerance in plantain. To the best of our knowledge, this is the first report of a global investigation on molecular responses of plantain to cold stress by

  13. Feynman motives of banana graphs

    CERN Document Server

    Aluffi, Paolo

    2008-01-01

    We consider the infinite family of Feynman graphs known as the ``banana graphs'' and compute explicitly the classes of the corresponding graph hypersurfaces in the Grothendieck ring of varieties as well as their Chern--Schwartz--MacPherson classes, using the classical Cremona transformation and the dual graph, and a blowup formula for characteristic classes. We outline the interesting similarities between these operations and we give formulae for cones obtained by simple operations on graphs. We formulate a positivity conjecture for characteristic classes of graph hypersurfaces and discuss briefly the effect of passing to noncommutative spacetime.

  14. Combating a Global Threat to a Clonal Crop: Banana Black Sigatoka Pathogen Pseudocercospora fijiensis (Synonym Mycosphaerella fijiensis) Genomes Reveal Clues for Disease Control

    Science.gov (United States)

    Aerts, Andrea; Carlier, Jean; Crane, Charles F.; de Vries, Ineke; Dietrich, Robert; Farmer, Andrew D.; Fortes Fereira, Claudia; Garcia, Suzana; Guzman, Mauricio; Hamelin, Richard C.; Lindquist, Erika A.; Mehrabi, Rahim; Quiros, Olman; Schmutz, Jeremy; Reynolds, Elizabeth; Scalliet, Gabriel; Souza, Manoel; Stergiopoulos, Ioannis; Van der Lee, Theo A. J.; De Wit, Pierre J. G. M.; Zapater, Marie-Françoise; Zwiers, Lute-Harm; Grigoriev, Igor V.; Goodwin, Stephen B.; Kema, Gert H. J.

    2016-01-01

    Black Sigatoka or black leaf streak disease, caused by the Dothideomycete fungus Pseudocercospora fijiensis (previously: Mycosphaerella fijiensis), is the most significant foliar disease of banana worldwide. Due to the lack of effective host resistance, management of this disease requires frequent fungicide applications, which greatly increase the economic and environmental costs to produce banana. Weekly applications in most banana plantations lead to rapid evolution of fungicide-resistant strains within populations causing disease-control failures throughout the world. Given its extremely high economic importance, two strains of P. fijiensis were sequenced and assembled with the aid of a new genetic linkage map. The 74-Mb genome of P. fijiensis is massively expanded by LTR retrotransposons, making it the largest genome within the Dothideomycetes. Melting-curve assays suggest that the genomes of two closely related members of the Sigatoka disease complex, P. eumusae and P. musae, also are expanded. Electrophoretic karyotyping and analyses of molecular markers in P. fijiensis field populations showed chromosome-length polymorphisms and high genetic diversity. Genetic differentiation was also detected using neutral markers, suggesting strong selection with limited gene flow at the studied geographic scale. Frequencies of fungicide resistance in fungicide-treated plantations were much higher than those in untreated wild-type P. fijiensis populations. A homologue of the Cladosporium fulvum Avr4 effector, PfAvr4, was identified in the P. fijiensis genome. Infiltration of the purified PfAVR4 protein into leaves of the resistant banana variety Calcutta 4 resulted in a hypersensitive-like response. This result suggests that Calcutta 4 could carry an unknown resistance gene recognizing PfAVR4. Besides adding to our understanding of the overall Dothideomycete genome structures, the P. fijiensis genome will aid in developing fungicide treatment schedules to combat this

  15. Combating a Global Threat to a Clonal Crop: Banana Black Sigatoka Pathogen Pseudocercospora fijiensis (Synonym Mycosphaerella fijiensis) Genomes Reveal Clues for Disease Control.

    Science.gov (United States)

    Arango Isaza, Rafael E; Diaz-Trujillo, Caucasella; Dhillon, Braham; Aerts, Andrea; Carlier, Jean; Crane, Charles F; V de Jong, Tristan; de Vries, Ineke; Dietrich, Robert; Farmer, Andrew D; Fortes Fereira, Claudia; Garcia, Suzana; Guzman, Mauricio; Hamelin, Richard C; Lindquist, Erika A; Mehrabi, Rahim; Quiros, Olman; Schmutz, Jeremy; Shapiro, Harris; Reynolds, Elizabeth; Scalliet, Gabriel; Souza, Manoel; Stergiopoulos, Ioannis; Van der Lee, Theo A J; De Wit, Pierre J G M; Zapater, Marie-Françoise; Zwiers, Lute-Harm; Grigoriev, Igor V; Goodwin, Stephen B; Kema, Gert H J

    2016-08-01

    Black Sigatoka or black leaf streak disease, caused by the Dothideomycete fungus Pseudocercospora fijiensis (previously: Mycosphaerella fijiensis), is the most significant foliar disease of banana worldwide. Due to the lack of effective host resistance, management of this disease requires frequent fungicide applications, which greatly increase the economic and environmental costs to produce banana. Weekly applications in most banana plantations lead to rapid evolution of fungicide-resistant strains within populations causing disease-control failures throughout the world. Given its extremely high economic importance, two strains of P. fijiensis were sequenced and assembled with the aid of a new genetic linkage map. The 74-Mb genome of P. fijiensis is massively expanded by LTR retrotransposons, making it the largest genome within the Dothideomycetes. Melting-curve assays suggest that the genomes of two closely related members of the Sigatoka disease complex, P. eumusae and P. musae, also are expanded. Electrophoretic karyotyping and analyses of molecular markers in P. fijiensis field populations showed chromosome-length polymorphisms and high genetic diversity. Genetic differentiation was also detected using neutral markers, suggesting strong selection with limited gene flow at the studied geographic scale. Frequencies of fungicide resistance in fungicide-treated plantations were much higher than those in untreated wild-type P. fijiensis populations. A homologue of the Cladosporium fulvum Avr4 effector, PfAvr4, was identified in the P. fijiensis genome. Infiltration of the purified PfAVR4 protein into leaves of the resistant banana variety Calcutta 4 resulted in a hypersensitive-like response. This result suggests that Calcutta 4 could carry an unknown resistance gene recognizing PfAVR4. Besides adding to our understanding of the overall Dothideomycete genome structures, the P. fijiensis genome will aid in developing fungicide treatment schedules to combat this

  16. 广西香蕉真菌性叶斑病病原菌种群结构分析%Investigations on the fungal populations associated with banana leaf spot diseases in Guangxi

    Institute of Scientific and Technical Information of China (English)

    林善海; 黄思良; 覃丽萍; 付岗; 岑贞陆; 谢玲

    2011-01-01

    2009年2-3月对广西香蕉主产区真菌性叶斑病病原进行抽样鉴定.结果显示,广西香蕉真菌性叶斑病病原至少有10种,主要病原为Cordana musae,分布广,检出率为56.77%~92.19%;Corynespora cassiicola和Deightoniella torulosa为次,两种病原菌均主要集中在南宁地区,检出率分别为32.34%和22.61%;叶斑病类型以单一病原侵染为主,检出率为78.13%~92.19%;复合侵染的叶斑病类型以2种病原共同侵染居多.%Investigations on banana leaf spot fungal pathogens were conducted in major banana production areas in Guangxi between February and March in 2009. The results showed that at least 10 fungal pathogens were found in the banana leaf spot samples. Cordana musae was the most frequently observed fungal pathogen with a detection rate of 56.77%-92.19% in the samples. Corynespora cassiicola and Deightoniella torulosa were mainly observed in the samples collected from the plantations at Nanning City, with the detection rates of 32.34% and 22. 61%, respectively. The banana leaf spots with only a single pathogen were predominant (78.13%-92.19%) compared to those with two or more fungal pathogens.

  17. COMPARAÇÃO DE MÉTODOS DE DESCONTAMINAÇÃO USADOS NA FASE INICIAL DO ESTABELECIMENTO EM CULTURA IN VITRO DE BANANA COMPARISON AMONG DECONTAMINATION METHODS AT INITIAL PHASE OF BANANA IN VITRO CULTURE

    Directory of Open Access Journals (Sweden)

    Lázaro José Chaves

    2007-09-01

    Full Text Available

    Quatro métodos de descontaminação de explantes de bananeira (Musa AAB cv. Maçã cultivados in vitro foram testados utilizando-se, como fonte de explantes, rizomas provenientes de bananais comerciais. Explantes de diferentes tamanhos obtidos de brotações axilares foram submetidos a diferentes concentrações e tempo em solução de NaOCl para descontaminação. Após redução para 8mm x 5mm x 5mm, os explantes foram transferidos para meio MS modificado e mantidos em câmara escura durante dez dias e, em seguida, foram levados para câmara de crescimento à temperatura de 28°C +/- 2°C e fotoperíodo de 16 horas. Os melhores resultados foram obtidos quando os tratamentos de descontaminação foram realizados em blocos de 40mm x 20mm x 20mm ou 40mm x 15mm x 15mm, com simples e dupla esterilizações, respectivamente, utilizando-se NaOCl 3,5% de cloro ativo. O aparecimento de fungos e bactérias foi mais acentuado nos primeiros quinze dias, entretanto, em concentração baixa de NaOC1 (1%, a contaminação foi quase total nos primeiros três dias (96,67%. Houve maior incidência de contaminação bacteriana do que fúngica.

    PALAVRAS-CHAVE: Banana cv. Maçã; cultura de tecidos; esterilização superficial; Musa AAB.

    Four decontamination methods for in vitro culture of banana (Musa AAB cv. Maçã explants were compared. Commercial crop rhizomes were used as the explant source. Different sizes of explants obtained from axillary buds were submersed in different concentrations of NaOCl for each different period of time. After being reduced to 8mm x 5mm x 5mm, explants were transferred to modified MS media, which were kept in the dark for 10 days, followed by growth chamber at 28°C +/- 2°C with 16h light. Best results were obtained with 40mm x 20

  18. Substituting Wheat Flour with Banana Skin Flour from Mixture Various Skin Types of Banana on Making Donuts

    Directory of Open Access Journals (Sweden)

    Renny Futeri

    2014-01-01

    Full Text Available Tropical forest plants is a very rich source of chemical compounds or bioactive efficacious . Many of the compounds potential as a source of raw materials in food processing . One is the banana plant , West Sumatra Padang and Bukittinggi is one area in Indonesia with banana . Generally people in West Sumatra just consume or eat the fruit and throw banana skin just because it is considered as waste ( waste banana peel . When the banana peel waste is left alone so do not rule out the possibility for the accumulation of garbage or waste banana peels , especially in the West Sumatra city of Padang and sekitarnya.Salah one solution that can be done is to harness and cultivate the banana peel waste into a material more useful for example in the manufacture of foodstuffs.Banana peel flour with all the treatments can produce flour banana peel . However, the manufacture of flour banana skin with the use of sodium metabisulfite 1% at 1 hour of soaking to get the best flour . Having obtained done banana peel flour donut -making flour substitute banana peel . The use of banana peel flour with different concentrations turned out to affect the organoleptic properties of the donut . Of hedonic organoleptic test , the results of the average value of the ratio between wheat flour with flour banana skin that gives the best results for color , aroma , and flavor that is a donut with banana peel flour ratio of 0 % to 100 % wheat flour and donuts with banana peel flour ratio 10 % with 90 % wheat flour , but the texture will be best results are donuts of banana peels can be made by substituting wheat flour with flour banana skin at 10 %. Carbohydrate content of flour banana skin with the use of sodium metabisulfite 1% at 1 hour soaking of 16.60 grams.

  19. CULTIVAR RELEASE - BRS Saturno – triticale cultivar

    Directory of Open Access Journals (Sweden)

    Alfredo do Nascimento Junior

    2011-01-01

    Full Text Available The triticale cultivar BRS Saturno was developed by Embrapa (Brazilian Agricultural Research Corporation inpartnership with the Fundação Pró-Sementes de Apoio à Pesquisa. It is the result of a cross between the genotypes PFT 512 and CEP28 – Guará, adapted to the environmental conditions of cultivation in southern Brazil.

  20. Antibacterial and antioxidant activities of Musa sp. leaf extracts against multidrug resistant clinical pathogens causing nosocomial infection

    Institute of Scientific and Technical Information of China (English)

    Ponmurugan Karuppiah; Muhammed Mustaffa

    2013-01-01

    Objective:To investigate different Musa sp. leave extracts of hexane, ethyl acetate and methanol were evaluated for antibacterial activity against multi-drug resistant pathogens causing nosocomial infection by agar well diffusion method and also antioxidant activities. Methods:The four different Musa species leaves were extracted with hexane, ethyl acetate and methanol. Antibacterial susceptibility test, minimum inhibitory concentration and minimum inhibitory bacterial concentration were determined by agar well diffusion method. Total phenolic content and in vitro antioxidant activity was determined. Results: All the Musa sp. extracts showed moderate antibacterial activities expect Musa paradisiaca with the inhibition zone ranging from 8.0 to 18.6 mm. Among four species ethyl acetate extracts of Musa paradisiaca showed highest activity against tested pathogens particularly E. coli, P. aeruginosa and Citrobacter sp. The minimum inhibitory concentrations were within the value of 15.63-250 µg/mL and minimum bactericidal concentrations were ranging from 31.25-250 µg/mL. Antioxidant activity of Musa acuminate exhibited maximum activity among other three Musa species. Conclusions: The present study concluded that among the different Musa species, Musa paradisiaca displayed efficient antibacterial activity followed by Musa acuminata against multi-drug resistant nosocomial infection causing pathogens. Further, an extensive study is needed to identify the bioactive compounds, mode of action and toxic effect in vivo of Musa sp.

  1. Development of the banana plants 'Prata Anã' and 'FHIA-01' under the effect of paclobutrazol applied on the soil.

    Science.gov (United States)

    Maia, Emanuel; Siqueira, Dalmo L; Salomão, Luiz C C; Peternelli, Luiz A; Ventrella, Marília C; Cavatte, Rithiely P Q

    2009-06-01

    Under some cultivation conditions, excessive growth of the pseudostem of banana plants can be considered a limiting factor, and thus, the use of growth regulators can constitute a valid alternative. This work aimed to evaluate the action of paclobutrazol on the growth of the pseudostem of banana plants and other characteristics of their development. An experiment was installed with five paclobutrazol doses (0; 0.5; 1.0; 1.5; and 2.0 g of a.i. plant-1) combined with two banana plant cultivars ('Prata Anã' and 'FHIA-01'). Paclobutrazol did not affect the number of days from planting to flowering, total leaf area and leaf emission rate. However, it increased leaf permanence on the plants and sucker number, reduced the foliar area of the leaves emitted after its application and provided an average reduction in pseudostem height of 26%.

  2. Genomic Integrity Detection of In Vitro Irradiated Banana Using Microsatellite Marker.

    Directory of Open Access Journals (Sweden)

    Nina Ratna Djuita

    2010-11-01

    Full Text Available Genomic Integrity Detection of In Vitro Irradiated Banana Using Microsatellite Marker. The research aims todetect genomic integrity of in vitro irradiated banana using microsatellite marker. These studies were done on bananacv. Pisang Mas irradiated by 15 Gy of gamma ray. The DNA was isolated from each accesion following Dixie.Amplification of DNA products were done by Perkin Elmer Gene Amp PCR 2400 using ten primers, and thenelectroforesis in agarose 1%. Finally a vertical polyacrylamide gel electroforesis was run and the products werevisualized by silver staining. The result shown that among the primers tested, eight primers produced clear, discrete,and reproducible bands. Number of DNA band exhibited ranging from one to two, following the ploidy level of pisangMas which is a diploid banana cultivar (AA. One band suggest homozygote allele while two bands showedheterozygote allele. Out of eight primers, six primers produced different allele among irradiated, in vitro, and in vivocontrol plant. Meanwhile, for the other two primers the allele were monomorph for all the accessions examined.Genomic modification was observed at all irradiated plants. The modification can happened at zygosity of certain allelethat may change from heterozygote to homozygote or vice versa. While modification in allele size that underlyinggenomic instability could be caused by several genetic events such as deletion, insertion, and amplification ofnucleotides.

  3. Chemical treatment of banana tree pseudostem particles aiming the production of particleboards

    Directory of Open Access Journals (Sweden)

    Bárbara Maria Ribeiro Guimarães

    2014-02-01

    Full Text Available Brazil is one of the major banana producers in the world, and consequently generates a great amount of residues from this crop, which could be utilized in particleboard production. The present work aimed to evaluate the effect of different chemical treatments of the particles of Musa sp. pseudostem, on their chemical and physical properties (basic density, and on the physical and mechanical properties of the particleboards produced with the same material. Five treatments were applied to the particles: 1 without treatment (control; 2 2% acetic acid; 3 0.5% NaOH; 4 acetone (1/1 in volume/volume; and 5 water. Panels with medium density were produced (0.70 g/cm³, bonded with 12% (in relation to the particle mass of urea-formaldehyde adhesive. The pressing cycle was conducted at 160º C, under 4 MPa, during 8 min. The results obtained indicated that the chemical treatments performed in the particles of banana tree pseudostem affected their chemical composition. The basic density of the particles was 0.10 g/cm³, and only the NaOH treatment led to its increase for values around 0.13 g/cm3. The panels produced with particles without treatment (control and particles treated with acetic acid presented the lowest values of water absorption and thickness swelling after 2 and 24 h of immersion. Mechanical properties of all the panels produced were below the minimum recommended by the standard CS 236-66 (COMMERCIAL STANDARD - CS, 1968.

  4. Deteminación de un índice de calidad del suelo en áreas productoras de banano (Musa x paradisiaca L.) de la vertiente del Pacifico de Panamá

    OpenAIRE

    Villarreal Nuñez, José Ezequiel

    2010-01-01

    El cultiu de la banana (Musa x paradisíaca L.) és un dels conreus agrícoles més importants en molts països tropicals i és el quart producte amb major volum d'exportació al món, només superat per l'arrós, el blat i el blat de moro (FAO, 2004). A Panamà, a pesar de l'aplicació de tècniques i insums d'alt cost, s'ha registrat una considerable reducció en la productivitat, Causa el canvi i deteriorament accelerat de les propietats físico-químiques i biològiques del sòl. Aquest treball es va reali...

  5. Effects of covering highland banana stumps with soil on banana weevil Cosmopolites sordidus (Coleoptera: Curculionidae) oviposition

    NARCIS (Netherlands)

    Masanza, M.; Gold, C.S.; Huis, van A.; Ragama, P.E.

    2005-01-01

    The effect of covering post-harvest banana stumps with soil on banana weevil Cosmopolites sordidus (Germar) oviposition levels was investigated at three locations, Sendusu, Kawanda Agricultural Research Institute (KARI) and Ntungamo district of southwestern Uganda. In the first experiment ovipositio

  6. La banane : de son origine à sa commercialisation

    Directory of Open Access Journals (Sweden)

    Lassois L.

    2009-01-01

    Full Text Available Banana: from origin to market. Cultivated bananas are giant herbaceous plants within the genus Musa. They are both sterile and parthenocarpic. There are well over a thousand domesticated Musa cultivars, they are mostly triploid (a few are diploid or tetraploid and are derived from crosses between two wild species, Musa acuminata and Musa balbisiana. In terms of production, bananas are the fourth agricultural product after rice, wheat, and maize. They constitute the basis of food security for many people. Cropping systems vary widely around the world and contrasting objectives are encountered: consumption by the producer, sale on local or national markets, export, etc. Cooking bananas, including plantains, must be distinguished from dessert bananas, which constitute a major international trade. This international trade started only in the early 1900s but it has since grown continuously. Banana is currently the most exported fruit, in terms of both value and quantity. Despite the high genetic diversity found within the genus Musa, the export market is mainly based on single Cavendish. There are major challenges to banana production from biotic or abiotic stresses to continue to meet the criteria of sustainability, quality and yield that are imposed.

  7. Adsorption Study on Moringa Oleifera Seeds and Musa Cavendish as Natural Water Purification Agents for Removal of Lead, Nickel and Cadmium from Drinking Water

    Science.gov (United States)

    Aziz, N. A. A.; Jayasuriya, N.; Fan, L.

    2016-07-01

    The effectiveness of plant based materials Moringa oleifera (Moringa) seeds and Musa cavendish (banana peel) for removing heavy metals namely lead (Pb), nickel (Ni) and cadmium (Cd) from contaminated groundwater was studied. Tests were carried out with individual and combined biomass at neutral pH condition on synthetic groundwater samples. The optimum biomass doses were determined as 200 mg/L for single biomass and 400 mg/L (in the ratio of 200 mg/L: 200 mg/L) for combined biomasses and used for adsorption isotherm studies with contact time of 30 minutes. Results showed that combined biomasses was able to met the Pb, Ni and Cd WHO standards from higher Pb, Ni and Cd initial concentrations which were up to 40 µg/L, 50 µg/L 9 µg/L, respectively compared to individual biomass of Moringa seed and banana peel. Moringa seeds exhibited the highest removal of Pb (81%) while the combined biomasses was most effective in removing Ni (74%) and Cd (97%) over wider their initial concentration ranges. The experimental data were linearized with Langmuir and Freundlich adsorption isotherm models. Freundlich model described the Pb adsorption better than the Langmuir model for all the tested biomasses. However, the Langmuir model fit better with the experimental data of Ni adsorption by Moringa seeds. Both models showed negligible differences in the coefficient of determination (R2) when applied for Ni and Cd adsorption on banana peel and combined biomasses, suggesting that there were multiple layers on the biomass interacting with the metals. Chemisorption is suggested to be involved in Pb adsorption for all tested biomasses as the value of nF calculated was lower than one. This type of adsorption could explain the phenomenon of different behavior of Pb removal and the higher Pb adsorption capacity (represented by KF values) compared to Ni and Cd. The study demonstrates that Moringa seeds, banana peel and their combination have the potential to be used as a natural alternative

  8. Research on Risks and Forecasting Countermeasures of Hainan Banana Industry

    OpenAIRE

    Liu, Yan-Qun; Zeng, Xiao-Hong; Fang, Jia

    2011-01-01

    Based on the overviews of the current conditions of Hainan banana industry, the research makes an analysis of the risks faced by Hainan banana industry. They are respectively marketing risks, natural risks, information risks and production risks. In order to promote a sustainable and rapid development of Hainan banana industry, Countermeasures are proposed in the research. The first is to strengthen the leading organization of forecasting mechanisms on banana industry. The second is to establ...

  9. Ganhos de eficiência fertilizante em bananeira sob irrigação e fertirrigação Enhancing nutrient use efficiency in banana due to irrigation and fertigation

    Directory of Open Access Journals (Sweden)

    Luiz Antonio Junqueira Teixeira

    2011-03-01

    Full Text Available O aumento da eficiência fertilizante tem sido cada vez mais almejado nas boas práticas de manejo da adubação de culturas, não somente buscando reduzir custos de produção, mas também reduzir possíveis impactos ambientais. Com o objetivo de avaliar a eficiência dos fertilizantes nitrogenados e potássicos no cultivo da bananeira, foram realizados dois experimentos de campo com a cultivar 'Nanicão' (Musa AAA, subgrupo Cavendish, no Planalto do Estado de São Paulo, durante quatro ciclos de produção. Esta região caracteriza-se por estação chuvosa no verão e seca no inverno. No primeiro experimento, avaliou-se a eficiência da aplicação das doses de N: 0; 200; 400 e 800 kg ha-1 e de K: 0; 300; 600 e 900 kg ha-1 de K2O, em sistema de cultivo irrigado, comparado ao de sequeiro. No segundo experimento, estudou-se a eficiência da adubação com N e K aplicada por fertirrigação e de modo convencional via solo. Nos dois experimentos, a irrigação e a fertirrigação foram feitas por microaspersão, empregando-se como fontes de N e de K nitrato de amônio e cloreto de potássio, respectivamente. A eficiência dos fertilizantes foi estimada pela relação entre a massa dos frutos produzidos por unidade de nutriente aplicado. Para a média das doses empregadas e dos ciclos de produção, observou-se que a irrigação incrementou a eficiência da adubação em relação ao sequeiro, em aproximadamente 36 % e 32 %, respectivamente, para nitrogênio e potássio. A aplicação de nitrogênio e potássio via fertirrigação promoveu aumento de 36 % na eficiência fertilizante em relação à adubação sólida convencional, via solo. Incrementos na eficiência dos fertilizantes decorrentes da irrigação e da fertirrigação possibilitaram diminuir os gastos com o uso destes insumos.The best nutritional practices aim to increase nutrient use efficiency in order to reduce production costs and environmental impacts. In order to evaluate

  10. Generation of Five New Musa Hybrids With Resistance To Black Sigatoka and High Yield

    Directory of Open Access Journals (Sweden)

    Ricardo Hernandez

    2007-01-01

    Full Text Available The ability to identify genetic variation is indispensable to effective management and use of genetic resources. This work is the first approach concerning to generation and genetic differentiation of new Musa hybrid lines obtained in INIVIT (Cuba, crossing Musa acuminata male diploid (AA x female triploid (ABB, genotypes selected by their resistant character to pests and diseases. From the obtained hybrids, only five were chosen because of their agronomic behavior and were genetically discriminated among them and also respect to the commercial clone FHIA-18 by AFLP polymorphism. Thanks to this prior genetic characterization Musa breeding programs could be consistently dinamised.

  11. Effects of particle size distribution on some physical, chemical and functional properties of unripe banana flour.

    Science.gov (United States)

    Savlak, Nazlı; Türker, Burcu; Yeşilkanat, Nazlıcan

    2016-12-15

    The objective of this study was to examine the effect of particle size distribution on physical, chemical and functional properties of unripe banana flour for the first time. A pure triploid (AAA group) of Musa acuminata subgroup Cavendish (°Brix;0.2, pH;4.73, titratable acidity; 0.56g/100g malic acid, total solids; 27.42%) which was supplied from Gazipaşa, Antalya, Turkey from October 2014 to October 2015 was used. Size fractions of physical, functional and antioxidant properties. Particle size significantly effected color, water absorbtion index and wettability. L(∗) value decreased, a(∗) and b(∗) values decreased by increasing particle size (r(2)=-0.94, r(2)=0.72, r(2)=0.73 respectively). Particles under 212μm had the lowest rate of wettability (83.40s). A negative correlation between particle size and wettability (r(2)=-0.75) and positive correlation between particle size and water absorption index (r(2)=0.94) was observed.

  12. CHANGES IN THE CHARACTERISTICS OF ‘PRATA’ BANANA TREATED WITH CYTOKININ AND GIBBERELLIN

    Directory of Open Access Journals (Sweden)

    JULIANA DOMINGUES LIMA

    Full Text Available ABSTRACT Plant growth regulators can influence fruit yield and quality. This study aimed to evaluate the effect of cytokinin and gibberelin on the agronomic and physicochemical characteristics of banana fruits cv. ‘Prata’ (Musa spp. AAB, according to the formation period and position in the bunch. The experiment was conducted in a completely randomized 2 x 5 factorial design, two periods of bunch development (summer and winter, five treatments and ten replicates. To study the effect of position in the bunch, split plot was adopted, considering in the plot, 2 x 5 factorial and in subplots, hand 1, hand 4 and last hand. Treatments consisted of 2 pulverizations with water, 150 mg L-1 cytokinin, 200 mg L-1 of gibberellic acid, 100 mg L-1 of cytokinin plus 200 mg L-1 of gibberellic acid and 200 mg L-1 of cytokinin plus 200 mg L-1 of gibberellic acid, applied from the fourth to the last hand of the bunch. Cytokinin and gibberellin, alone or associated, regardless of formation period and position, did not affect the size and physicochemical characteristics of fruits, only delayed the bunch harvest.

  13. Hormonal and Hydroxycinnamic Acids Profiles in Banana Leaves in Response to Various Periods of Water Stress

    Directory of Open Access Journals (Sweden)

    Jalel Mahouachi

    2014-01-01

    Full Text Available The pattern of change in the endogenous levels of several plant hormones and hydroxycinnamic acids in addition to growth and photosynthetic performance was investigated in banana plants (Musa acuminata cv. “Grand Nain” subjected to various cycles of drought. Water stress was imposed by withholding irrigation for six periods with subsequent rehydration. Data showed an increase in abscisic acid (ABA and indole-3-acetic acid (IAA levels, a transient increase in salicylic acid (SA concentration, and no changes in jasmonic acid (JA after each period of drought. Moreover, the levels of ferulic (FA and cinnamic acids (CA were increased, and plant growth and leaf gas exchange parameters were decreased by drought conditions. Overall, data suggest an involvement of hormones and hydroxycinnamic acids in plant avoidance of tissue dehydration. The increase in IAA concentration might alleviate the senescence of survival leaves and maintained cell elongation, and the accumulation of FA and CA could play a key role as a mechanism of photoprotection through leaf folding, contributing to the effect of ABA on inducing stomatal closure. Data also suggest that the role of SA similarly to JA might be limited to a transient and rapid increase at the onset of the first period of stress.

  14. I Have a Banana Tree in My Classroom

    Science.gov (United States)

    Williams, Patricia A.

    2007-01-01

    When the banana is growing, the broadest part of the banana is located at the bottom, while the tapered end points upward. It appears upside down, however, from the banana tree's perspective, it is growing right side up. The author observes that the students in her classroom labeled by society as "at risk," are also, in a sense, "upside down."…

  15. Research on Risks and Forecasting Countermeasures of Hainan Banana Industry

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Based on the overviews of the current conditions of Hainan banana industry,the research makes an analysis of the risks faced by Hainan banana industry.They are respectively marketing risks,natural risks,information risks and production risks.In order to promote a sustainable and rapid development of Hainan banana industry,countermeasures are proposed in the research.The first is to strengthen the leading organization of forecasting mechanisms on banana industry.The second is to establish the forecasting mechanisms on banana industry,including four aspects.They are establishing the subordinate forecasting systems on Hainan banana industry;constructing information collecting and checking mechanisms of banana industry;establishing information analysis and decision-making systems and constructing information distribution and information sharing systems.The third is to promote the construction of urgency dealing abilities of banana industry.The fourth is to further perfect the risk-defending and protecting systems of banana industry in Hainan.The fifth is to accelerate the standard generation of banana to improve marketing competence.The sixth is to accelerate the development of intermediate agents to improve the organization degrees.And the last one is to put emphasis on the tech-training courses on banana planting and production to improve the technical quality of banana industry.

  16. Comparative biochemical analysis after steam pretreatment of lignocellulosic agricultural waste biomass from Williams Cavendish banana plant (Triploid Musa AAA group).

    Science.gov (United States)

    Kamdem, Irénée; Jacquet, Nicolas; Tiappi, Florian Mathias; Hiligsmann, Serge; Vanderghem, Caroline; Richel, Aurore; Jacques, Philippe; Thonart, Philippe

    2015-11-01

    The accessibility of fermentable substrates to enzymes is a limiting factor for the efficient bioconversion of agricultural wastes in the context of sustainable development. This paper presents the results of a biochemical analysis performed on six combined morphological parts of Williams Cavendish Lignocellulosic Biomass (WCLB) after steam cracking (SC) and steam explosion (SE) pretreatments. Solid (S) and liquid (L) fractions (Fs) obtained from SC pretreatment performed at 180°C (SLFSC180) and 210°C (SLFSC210) generated, after diluted acid hydrolysis, the highest proportions of neutral sugar (NS) contents, specifically 52.82 ± 3.51 and 49.78 ± 1.39%w/w WCLB dry matter (DM), respectively. The highest proportions of glucose were found in SFSC210 (53.56 ± 1.33%w/w DM) and SFSC180 (44.47 ± 0.00%w/w DM), while the lowest was found in unpretreated WCLB (22.70 ± 0.71%w/w DM). Total NS content assessed in each LF immediately after SC and SE pretreatments was less than 2%w/w of the LF DM, thus revealing minor acid autohydrolysis consequently leading to minor NS production during the steam pretreatment. WCLB subjected to SC at 210 °C (SC210) generated up to 2.7-fold bioaccessible glucan and xylan. SC and SE pretreatments showed potential for the deconstruction of WCLB (delignification, depolymerization, decrystallization and deacetylation), enhancing its enzymatic hydrolysis. The concentrations of enzymatic inhibitors, such as 2-furfuraldehyde and 5-(hydroxymethyl)furfural from LFSC210, were the highest (41 and 21 µg ml(-1), respectively). This study shows that steam pretreatments in general and SC210 in particular are required for efficient bioconversion of WCLB. Yet, biotransformation through biochemical processes (e.g., anaerobic digestion) must be performed to assess the efficiency of these pretreatments.

  17. Remote quality monitoring in the banana chain.

    Science.gov (United States)

    Jedermann, Reiner; Praeger, Ulrike; Geyer, Martin; Lang, Walter

    2014-06-13

    Quality problems occurring during or after sea transportation of bananas in refrigerated containers are mainly caused by insufficient cooling and non-optimal atmospheric conditions, but also by the heat generated by respiration activity. Tools to measure and evaluate these effects can largely help to reduce losses along the banana supply chain. The presented green life model provides a tool to predict the effect of deviating temperature, relative humidity, and CO2 and O2 gas concentrations on the storage stability of bananas. A second thermal model allows evaluation of the cooling efficiency, the effect of changes in packaging and stowage and the amount of respiration heat from the measured temperature curves. Spontaneous ripening causes higher respiration heat and CO2 production rate. The resulting risk for creation of hot spots increases in positions in which the respiration heat exceeds the available cooling capacity. In case studies on the transport of bananas from Costa Rica to Europe, we validated the models and showed how they can be applied to generate automated warning messages for containers with reduced banana green life or with temperature problems and also for remote monitoring of the ripening process inside the container.

  18. Antidiarrhoeal Activity of Musa paradisiaca Sap in Wistar Rats.

    Science.gov (United States)

    Yakubu, Musa T; Nurudeen, Quadri O; Salimon, Saoban S; Yakubu, Monsurat O; Jimoh, Rukayat O; Nafiu, Mikhail O; Akanji, Musbau A; Oladiji, Adenike T; Williams, Felicia E

    2015-01-01

    The folkloric claim of Musa paradisiaca sap in the management of diarrhoea is yet to be substantiated or refuted with scientific data. Therefore, the aim of the current study was to screen the sap of M. paradisiaca for both its secondary metabolites and antidiarrhoeal activity at 0.25, 0.50, and 1.00 mL in rats. Secondary metabolites were screened using standard methods while the antidiarrhoeal activity was done by adopting the castor oil-induced diarrhoeal, castor oil-induced enteropooling, and gastrointestinal motility models. The sap contained flavonoids, phenolics, saponins, alkaloids, tannins, and steroids while cardiac glycosides, anthraquinones, triterpenes, cardenolides, and dienolides were not detected. In the castor oil-induced diarrhoeal model, the sap significantly (P sap were accompanied by increase in inhibition of intestinal fluid content in the enteropooling model. The sap decreased the charcoal meal transit in the gastrointestinal motility model. In all the models, the 1.00 mL of the sap produced changes that compared well with the reference drugs. Overall, the antidiarrhoeal activity of Musa paradisiaca sap attributed to the presence of alkaloids, phenolics, flavonoids, and/or saponins which may involve, among others, enhancing fluid and electrolyte absorption through de novo synthesis of the sodium potassium ATPase and/or reduced nitric oxide levels.

  19. Development of expressed sequence tag and expressed sequence tag–simple sequence repeat marker resources for Musa acuminata

    Science.gov (United States)

    Passos, Marco A. N.; de Oliveira Cruz, Viviane; Emediato, Flavia L.; de Camargo Teixeira, Cristiane; Souza, Manoel T.; Matsumoto, Takashi; Rennó Azevedo, Vânia C.; Ferreira, Claudia F.; Amorim, Edson P.; de Alencar Figueiredo, Lucio Flavio; Martins, Natalia F.; de Jesus Barbosa Cavalcante, Maria; Baurens, Franc-Christophe; da Silva, Orzenil Bonfim; Pappas, Georgios J.; Pignolet, Luc; Abadie, Catherine; Ciampi, Ana Y.; Piffanelli, Pietro; Miller, Robert N. G.

    2012-01-01

    Background and aims Banana (Musa acuminata) is a crop contributing to global food security. Many varieties lack resistance to biotic stresses, due to sterility and narrow genetic background. The objective of this study was to develop an expressed sequence tag (EST) database of transcripts expressed during compatible and incompatible banana–Mycosphaerella fijiensis (Mf) interactions. Black leaf streak disease (BLSD), caused by Mf, is a destructive disease of banana. Microsatellite markers were developed as a resource for crop improvement. Methodology cDNA libraries were constructed from in vitro-infected leaves from BLSD-resistant M. acuminata ssp. burmaniccoides Calcutta 4 (MAC4) and susceptible M. acuminata cv. Cavendish Grande Naine (MACV). Clones were 5′-end Sanger sequenced, ESTs assembled with TGICL and unigenes annotated using BLAST, Blast2GO and InterProScan. Mreps was used to screen for simple sequence repeats (SSRs), with markers evaluated for polymorphism using 20 diploid (AA) M. acuminata accessions contrasting in resistance to Mycosphaerella leaf spot diseases. Principal results A total of 9333 high-quality ESTs were obtained for MAC4 and 3964 for MACV, which assembled into 3995 unigenes. Of these, 2592 displayed homology to genes encoding proteins with known or putative function, and 266 to genes encoding proteins with unknown function. Gene ontology (GO) classification identified 543 GO terms, 2300 unigenes were assigned to EuKaryotic orthologous group categories and 312 mapped to Kyoto Encyclopedia of Genes and Genomes pathways. A total of 624 SSR loci were identified, with trinucleotide repeat motifs the most abundant in MAC4 (54.1 %) and MACV (57.6 %). Polymorphism across M. acuminata accessions was observed with 75 markers. Alleles per polymorphic locus ranged from 2 to 8, totalling 289. The polymorphism information content ranged from 0.08 to 0.81. Conclusions This EST collection offers a resource for studying functional genes, including

  20. María Rosa Lojo, La musa ribelle. Il romanzo di Victoria Ocampo.

    OpenAIRE

    2011-01-01

    María Rosa Lojo, La musa ribelle. Il romanzo di Victoria Ocampo. Traduzione di Immacolata Forlano. Introduzione di Rosa Maria Grillo, (Salerno, Oèdipus, 2010, 187 pp., ISBN – 10 8873411352)di Camilla Cattarulla

  1. Antiurolithiatic and antioxidant efficacy of Musa paradisiaca pseudostem on ethylene glycol-induced nephrolithiasis in rat

    Directory of Open Access Journals (Sweden)

    Padma Nibash Panigrahi

    2017-01-01

    Conclusions: The present findings demonstrate the efficacy of MUSA in EG-induced urolithiasis, which might be mediated through inhibiting various pathways involved in renal calcium oxalate formation, antioxidant effect, and potential to inhibit biochemical markers of renal impairment.

  2. Efeito da interação entre carvão ativado e N6-benzilaminopurina na propagação in vitro de bananeira, cv. Grand Naine (AAA Interaction effect between activated charcoal and N6-benzylaminopurine in the in vitro propagation of banana, cultivar Grand Naine (AAA

    Directory of Open Access Journals (Sweden)

    Frederico Henrique da Silva Costa

    2006-08-01

    Full Text Available O carvão ativado possui a propriedade de adsorver os compostos fenólicos liberados pela oxidação dos tecidos lesionados durante o cultivo in vitro. O objetivo deste trabalho foi avaliar os efeitos da interação entre o carvão ativado e diferentes concentrações de N6-benzilaminopurina (BAP na multiplicação in vitro da bananeira, cv. Grande Naine (AAA. O meio de cultura utilizado foi o MS, solidificado com 5 g.L-1 de ágar. O cultivo foi mantido em sala de crescimento a 25±2ºC, fotoperíodo de 16 horas e intensidade luminosa de 30 mmol.m-2s-1. Foram avaliadas a presença e a ausência de carvão ativado (0 e 3 g.L-1 e quatro concentrações de BAP (0; 2; 4 e 6 mg.L-1 no meio de cultura. O delineamento foi inteiramente casualizado, com cinco repetições, em um sistema fatorial 2x4. Os explantes foram avaliados a cada 30 dias, por um período de quatro subcultivos. Após cada subcultivo, o comprimento de brotações, a taxa de multiplicação, o vigor, o nível de oxidação das brotações emitidas e o número de raízes formadas foram avaliados. Independentemente das concentrações de BAP, o carvão ativado influenciou significativamente em todas as variáveis analisadas. De maneira geral, a adição de carvão ativado afetou negativamente a taxa de multiplicação, embora tenha melhorado o vigor e o número de raízes e diminuído a oxidação dos explantes. Na ausência de carvão ativado, o BAP proporcionou as maiores taxas de multiplicação das brotações.Active charcoal has the capacity to fix phenolic compounds released by the oxidation of manipulated tissues during in vitro cultivation. The present work objective was to evaluate the interaction effects between active charcoal, combined to different BAP concentrations in the in vitro banana propagation, cv. Grand Nain (AAA. The culture medium used was the MS, solidified with 5 g.L-1 agar. Cultures were kept in a growth room at 25 ± 2ºC with a photoperiod of 16 h at 30 mmol

  3. Professor Schmidt’s Banana Cake Recipe

    DEFF Research Database (Denmark)

    Schmidt, Bjarne

    2016-01-01

    In this paper we present the recipe of the (not yet) famous banana cake. The recipe has a solid background in the literature, but our experiments have shown that the outcome can be improved significantly by doping the batter with different kinds of ingredients.......In this paper we present the recipe of the (not yet) famous banana cake. The recipe has a solid background in the literature, but our experiments have shown that the outcome can be improved significantly by doping the batter with different kinds of ingredients....

  4. Silicon Isotope Fractionation by Banana Under Continuous Nutrient and Silica Flux

    Science.gov (United States)

    Opfergelt, S.; Cardinal, D.; Henriet, C.; Delvaux, B.; André, L.

    2004-12-01

    Silicon is absorbed by plants as aqueous H4SiO4 with other essential nutrients, and precipitates in aerial parts of the plant as phytolith, a biogenic opal. Phytoliths are restored to the soil by decomposition of organic debris from plant material. The role of higher plants in the biogeochemical cycle of silicon is therefore major although it is still poorly studied. Biomineralization processes are known to fractionate the three stable silicon isotopes with a preferential uptake of light isotopes. Therefore, following some preliminary results from Douthitt (1982), and studies presented in recent conferences (Ziegler et al., 2002; Ding et al., 2003), we suspect that phytolith production by plants could also fractionate the silicon isotopes. Inversely, intensity of phytolith-related isotopic fractionations might contribute to a better understanding of the soil-plant silicon cycle. Our study focused on banana, a silicon accumulating plant (>1% Si, dry weight).Musa acuminata cv Grande Naine has been grown in hydroponics under controlled conditions (light, temperature, humidity, nutrients) during six weeks. The nutrient supply was kept constant: three batches of five plants were grown with a continuous nutrient solution flow of 5, 50 and 100 ppm SiO2 respectively. Si isotopic compositions were measured in the source solution, and in silica extracted from the various parts of banana (roots, pseudostems, midribs and petioles, leaves), using a Nu Plasma multicollector mass spectrometer (MC-ICP-MS) operating in dry plasma mode. The results are expressed as δ 29Si relatively to the NBS28 standard, with an average precision of ± 0.03‰ . Silicon contents and morphological studies of phytoliths were also achieved. Banana δ 29Si varied between -0.18 and -0.76‰ with a source solution at -0.02‰ . Values of δ 29Si were less fractionated, relatively to the nutrient solution, in roots, where no phytoliths have been observed until now, than in upper parts of banana where

  5. Pessegueiro: cultivar BRS kampai

    Directory of Open Access Journals (Sweden)

    Maria do Carmo Bassols Raseira

    2010-12-01

    Full Text Available RESUMO O programa de Melhoramento Genético de Pessegueiros da Embrapa Clima Temperado tem, entre seus objetivos, a obtenção de cultivares produtoras de frutas para consumo in natura, com características que satisfaçam às exigências dos consumidores. Alguns dos grandes centros consumidores, como é o caso de São Paulo e Curitiba, preferem pêssegos de polpa branca e sabor doce. A cultivar BRS Kampai, obtida de um cruzamento entre 'Chimarrita' e 'Flordaprince', alia a baixa necessidade em frio, o que é uma vantagem em regiões subtropicais, à boa aparência, com sabor superior a qualquer um dos parentais. A colheita dos frutos desta cultivar inicia-se geralmente, em meados de novembro, em Pelotas-RS (em Atibaia, São Paulo, inicia-se na segunda quinzena de outubro, poucos dias antes das cultivares Rubimel (polpa amarela e Premier (polpa branca, sendo ótima substituta para esta última.

  6. Utilização de Ecolife® e Acibenzolar-s-metil (ASM no controle da antracnose da banana em pós-colheita Use of Ecolife® and Acibenzolar-S-metil (ASM on the control of antracnosis in banana post-harvest

    Directory of Open Access Journals (Sweden)

    Luciano Marinho Furtado

    2010-09-01

    Full Text Available O objetivo deste trabalho foi avaliar a ação dos produtos ASM e Ecolife no controle da antracnose pós-colheita em frutos de banana. Frutos sadios de banana, variedades maçã, prata, pacovan e cacau , em fase intermediária de maturação, foram imersos previamente em soluções de ASM e Ecolife e inoculados com Colletotrichum musae. Utilizou-se o delineamento inteiramente casualizado, em esquema fatorial com quatro repetições. Os resultados apresentados demonstraram a eficácia dos produtos nas concentrações de 5 ml/ L (Ecolife e 0,50g/ L (ASM no controle da antracnose nas variedades analisa das. A variedade cacau apresentou menor lesão quando tratada com o Ecolife (5,79 mm. Com relação ao efeito do ASM, a bana na prata demonstrou um melhor resultado, com tamanho médio de lesão de 5,62 mm. Com o decorrer do processo de maturação dos frutos houve um decréscimo na severidade da doença nas quatro variedades estudadas, exceto no tratamento testemunha, que continuou apresentando aumento no tamanho das lesões nos frutos e atingir a polpa ao final da maturação.The aim of this work was to evaluate the effects of ASM and Ecolife on the control of post-harvest antracnosis in banana. Banana fruits of Maçã, Prata, Pacovan and Figo varieties, at intermediate stage of maturation, were immersed in solutions of ASM and Ecolife and inoculated with Colletotrichum musae A completely randomized design was used, at a factorial scheme 4x2 (four varieties x two products with four replicates. The presented results demonstrated the susceptibility of the fruits to the disease, mainly Maça variety with lesion of 17,99 mm. It was demosntrated the effectiveness of products at concentrations of 5ml. L-1 (Ecolife and 0.50g. L-1 (ASM on antracnosis control. The Figo variety presented the smallest injuries when treated with Ecolife (5.79 mm. Regarding ASM effects, Prata variety demonstrated the best performance, with 5.62 mm of injuries diameter

  7. Evaluation of vegetable extracts for control of the pathogenic fungi in banana and strawberry in post harvest storage Evaluación de extractos vegetales para manejo de hongos patógenos en banano y fresa almacenados

    Directory of Open Access Journals (Sweden)

    Bonilla Correa Carmen Rosa

    2006-12-01

    Full Text Available

    This study was carried out get knowledge about fungicidal properties of vegetable extracts to the control of the pathogenyc fungi (Colletotrichum musae and Botrytis cinerea in banana (Musa sapientum and strawberry (Fragaria sp during the pos harvest storage, at the National University of Colombia headquarters in Palmira. Ethanolics extracts of the following vegetable species were evaluated: Plantago major L, Ruta graveolens L, Lippia alba (Mill NE BROS, Pteridium aquilinum (Kaulf Maxon. The extracts were obtained by the percolation method starting from the macerated material (100 g and 1000 ml of solvent. The fungi was isolated from the strawberry (Fragaria sp affected by B. cinerea and banana Gross mitchel affected by C. musae. To evaluate the control of the fungi C. musae and B. cinerea sixteen treatments were used (four extracts of plants, two extraction media, two dilutions and three repetitions. Three control treatments were added: absolute (PDA; solvent (PDA + ethanol or water; and chemical (PDA + chemical fungicide. The dilutions were prepared with the solvent used to obtain the extract. The evaluation in vitro was maid with ethanolics extracts diluted at 25%, because these presented the best results in the previous tests. The results indicate that the ethanolic solvent decreased the development of the fungi in 68%. The fruits in which the etanolic extract of ruda and pronto alivio was applied presented bigger incidence and grade of severity.

    Keys words: Vegetable extract; patogenic fungi; Colletotrichum musae; Botrytis cinérea; post harvest

    En la Universidad Nacional de Colombia -Sede Palmira, se obtuvieron extractos etanólicos (1.000 ml, macerados (100 g de llantén (Plantago major L, ruda (Ruta graveolens L, pronto alivio [Lippia alba (Mill NE BROS] y helecho marranero [Pteridium aquilinum (Kaulf Maxon]. Para la evaluación del control

  8. Identification of Differentially-Expressed Genes in Response to Mycosphaerella fijiensis in the Resistant Musa Accession 'Calcutta-4' Using Suppression Subtractive Hybridization.

    Science.gov (United States)

    Sánchez Timm, Eduardo; Hidalgo Pardo, Lisette; Pacheco Coello, Ricardo; Chávez Navarrete, Tatiana; Navarrete Villegas, Oscar; Santos Ordóñez, Efrén

    2016-01-01

    . Genes with different functions may play a role in plant response to the disease. The present study suggests a fine up regulation of these genes that might be needed to perform an incompatible interaction. Further gene functional studies need to be performed to validate their use as candidate resistance genes in susceptible banana cultivars.

  9. Ecuadorian banana farms should consider organic banana with low price risks in their land-use portfolios.

    Science.gov (United States)

    Castro, Luz Maria; Calvas, Baltazar; Knoke, Thomas

    2015-01-01

    Organic farming is a more environmentally friendly form of land use than conventional agriculture. However, recent studies point out production tradeoffs that often prevent the adoption of such practices by farmers. Our study shows with the example of organic banana production in Ecuador that economic tradeoffs depend much on the approach of the analysis. We test, if organic banana should be included in economic land-use portfolios, which indicate how much of the land is provided for which type of land-use. We use time series data for productivity and prices over 30 years to compute the economic return (as annualized net present value) and its volatility (with standard deviation as risk measure) for eight crops to derive land-use portfolios for different levels of risk, which maximize economic return. We find that organic banana is included in land-use portfolios for almost every level of accepted risk with proportions from 1% to maximally 32%, even if the same high uncertainty as for conventional banana is simulated for organic banana. A more realistic, lower simulated price risk increased the proportion of organic banana substantially to up to 57% and increased annual economic returns by up to US$ 187 per ha. Under an assumed integration of both markets, for organic and conventional banana, simulated by an increased coefficient of correlation of economic return from organic and conventional banana (ρ up to +0.7), organic banana holds significant portions in the land-use portfolios tested only, if a low price risk of organic banana is considered. We conclude that uncertainty is a key issue for the adoption of organic banana. As historic data support a low price risk for organic banana compared to conventional banana, Ecuadorian farmers should consider organic banana as an advantageous land-use option in their land-use portfolios.

  10. Ecuadorian Banana Farms Should Consider Organic Banana with Low Price Risks in Their Land-Use Portfolios

    Science.gov (United States)

    Castro, Luz Maria; Calvas, Baltazar; Knoke, Thomas

    2015-01-01

    Organic farming is a more environmentally friendly form of land use than conventional agriculture. However, recent studies point out production tradeoffs that often prevent the adoption of such practices by farmers. Our study shows with the example of organic banana production in Ecuador that economic tradeoffs depend much on the approach of the analysis. We test, if organic banana should be included in economic land-use portfolios, which indicate how much of the land is provided for which type of land-use. We use time series data for productivity and prices over 30 years to compute the economic return (as annualized net present value) and its volatility (with standard deviation as risk measure) for eight crops to derive land-use portfolios for different levels of risk, which maximize economic return. We find that organic banana is included in land-use portfolios for almost every level of accepted risk with proportions from 1% to maximally 32%, even if the same high uncertainty as for conventional banana is simulated for organic banana. A more realistic, lower simulated price risk increased the proportion of organic banana substantially to up to 57% and increased annual economic returns by up to US$ 187 per ha. Under an assumed integration of both markets, for organic and conventional banana, simulated by an increased coefficient of correlation of economic return from organic and conventional banana (ρ up to +0.7), organic banana holds significant portions in the land-use portfolios tested only, if a low price risk of organic banana is considered. We conclude that uncertainty is a key issue for the adoption of organic banana. As historic data support a low price risk for organic banana compared to conventional banana, Ecuadorian farmers should consider organic banana as an advantageous land-use option in their land-use portfolios. PMID:25799506

  11. Trait-based characterisation of soil exploitation strategies of banana, weeds and cover plant species.

    Science.gov (United States)

    Tardy, Florence; Damour, Gaëlle; Dorel, Marc; Moreau, Delphine

    2017-01-01

    Cover plants can be introduced in cropping systems to provide agroecosystem services, including weed control via competition for resources. There is currently no consensus on how to identify the best cover plant species, while trait-based approaches are promising for screening plant species due to their agroecosystem service provision potential. This study was carried out to characterize soil exploitation strategies of cover plant species in banana agroecosystems using a trait-based approach, and in turn identify cover plant species with a high weed control potential via competition for soil resources in banana cropping systems. A field experiment was conducted on 17 cover plant species, two weed species and two banana cultivars grown individually. Four functional traits were measured. Two of them (i.e., the size of the zone explored by roots and the root impact density) were used to characterize root system soil exploration patterns. Two other traits (i.e., specific root length and root diameter) were used to characterize resource acquisition within the soil zone explored by the roots. All studied traits exhibited marked variations among species. The findings suggested a trade-off between the abilities of species to develop a limited number of large diameter roots exploring a large soil zone versus many thin roots exploring a smaller soil zone. Three soil-resource exploitation strategies were identified among species: (i) with large diameter roots that explore a large soil zone; (ii) with small diameter roots and a high specific length that explore a smaller soil zone; and (iii) with a high total root-impact density and an intermediate specific root length that explore the uppermost soil layers. Interestingly, in our panel of species, no correlations with regard to belowground and aboveground strategies were noted: species with an acquisitive belowground strategy could display an acquisitive or a conservative aboveground strategy. The findings of this study

  12. Trait-based characterisation of soil exploitation strategies of banana, weeds and cover plant species

    Science.gov (United States)

    Tardy, Florence; Damour, Gaëlle; Dorel, Marc; Moreau, Delphine

    2017-01-01

    Cover plants can be introduced in cropping systems to provide agroecosystem services, including weed control via competition for resources. There is currently no consensus on how to identify the best cover plant species, while trait-based approaches are promising for screening plant species due to their agroecosystem service provision potential. This study was carried out to characterize soil exploitation strategies of cover plant species in banana agroecosystems using a trait-based approach, and in turn identify cover plant species with a high weed control potential via competition for soil resources in banana cropping systems. A field experiment was conducted on 17 cover plant species, two weed species and two banana cultivars grown individually. Four functional traits were measured. Two of them (i.e., the size of the zone explored by roots and the root impact density) were used to characterize root system soil exploration patterns. Two other traits (i.e., specific root length and root diameter) were used to characterize resource acquisition within the soil zone explored by the roots. All studied traits exhibited marked variations among species. The findings suggested a trade-off between the abilities of species to develop a limited number of large diameter roots exploring a large soil zone versus many thin roots exploring a smaller soil zone. Three soil-resource exploitation strategies were identified among species: (i) with large diameter roots that explore a large soil zone; (ii) with small diameter roots and a high specific length that explore a smaller soil zone; and (iii) with a high total root-impact density and an intermediate specific root length that explore the uppermost soil layers. Interestingly, in our panel of species, no correlations with regard to belowground and aboveground strategies were noted: species with an acquisitive belowground strategy could display an acquisitive or a conservative aboveground strategy. The findings of this study

  13. Banana Algebra: Compositional syntactic language extension

    DEFF Research Database (Denmark)

    Andersen, Jacob; Brabrand, Claus; Christiansen, David Raymond

    2013-01-01

    algebra as presented in the paper is implemented as the Banana Algebra Tool which may be used to syntactically extend languages in an incremental and modular fashion via algebraic composition of previously defined languages and transformations. We demonstrate and evaluate the tool via several kinds...

  14. 食品防腐剂、消毒剂和臭氧对香蕉货架寿命的影响%The Effect of Preservatives, Disinfectants and Ozone on Shelf Life of Banana

    Institute of Scientific and Technical Information of China (English)

    王向阳

    2012-01-01

    炭疽病是影响香蕉采后货架寿命限制因子.为了寻找国家标准允许使用的控制香蕉采后炭疽病的方法,从腐烂香蕉上分离炭疽病菌,并进行鉴定.对纯水中炭疽