WorldWideScience

Sample records for bagre ariopsis felis

  1. Metales pesados en tejido muscular del bagre Ariopsis felis en el sur del golfo de México (2001-2004) Heavy metals in muscular tissue of the catfish, Ariopsis felis, in the southern Gulfof México (2001-2004)

    OpenAIRE

    Felipe Vázquez; Tomás R Florville-Alejandre; Miguel Herrera; Luz María Díaz de León

    2008-01-01

    Se analizó el contenido de metales pesados en tejido muscular del bagre, Ariopsis felis en el sur del golfo de México durante el período 2001-2004. La investigación fue efectuada buscando establecer un marco de referencia ambiental para este organismo. La concentración metálica siguió el orden: Hg < Co < Pb < Ni < V < Cr. Fue observado un decremento en contenido de cobalto y vanadio, incremento en contenido de níquel, mercurio y cromo, y ausencia de estos comportamientos para contenido de plo...

  2. Metales pesados en tejido muscular del bagre Ariopsis felis en el sur del golfo de México (2001-2004 Heavy metals in muscular tissue of the catfish, Ariopsis felis, in the southern Gulfof México (2001-2004

    Directory of Open Access Journals (Sweden)

    Felipe Vázquez

    2008-01-01

    Full Text Available Se analizó el contenido de metales pesados en tejido muscular del bagre, Ariopsis felis en el sur del golfo de México durante el período 2001-2004. La investigación fue efectuada buscando establecer un marco de referencia ambiental para este organismo. La concentración metálica siguió el orden: Hg We analyzed the heavy metal content in the muscular tissue of the catfish, Ariopsis felis, in the southern Gulf of México between 2001 and 2004. The research was done in order to establish an environmental frame of reference for this organism. The metal concentration was as follows: Hg < Co < Pb < Ni < V < Cr. Cobalt and vanadium contents were found to decrease and those of nickel, mercury, and chromium to ulerease; however, neither of these trends was observed for the lead content. One-way analyses of variance con-firm significant temporal variation only for cobalt, mercury, lead, and vanadium. Significant linear correlation coefficients (p ≤ 0.05 were found for Co-V, Cr-Ni, Cr-Pb, Co-Hg, Ni-V, and V-Pb. The first three associa-tions showed positive correlations, whereas the remaining ones had negative correlations. A factor analysis grouped the studied metals depending on their origins. The metal levels found in the muscular tissue of A. felis from the southern Gulf of México were lower than those set by national and international regulations.

  3. Response of CYP1A Gene expression in fish liver of catfish (Ariopsis felis) from Gulf of Mexico and their relationship with the genetic variability.

    Science.gov (United States)

    Zapata-Perez, Omar; Sanchez-Teyer, Lorenzo F; Perez-Nunez, Maria T; Arroyo-Herrera, Ana L; Moreno, Adriana Quiroz; Albores-Medina, Arnulfo

    2010-01-01

    We determined the hepatic Cytochrome P4501A (CYP1A) mRNA and Ethoxyresorufin-O-deethylase (EROD) activities in the fish, Ariopsis felis, from highly polluted to relatively pristine regions in the southwest Gulf of Mexico and their relationship with the genetic polymorphisms of the same fish. We hypothesized that a high genetic variation reflects interindividual variability in levels of CYP1A mRNA underlying the pathway culminating in EROD induction caused by the environmental contaminants. Catfish from Laguna de Mecoacan exhibited marked induction of CYP1A mRNA and high levels of hepatic EROD activities, whereas fish from Laguna de Celestun showed no induction of CYP1A mRNA and moderately low levels of EROD activities. In contrast, the similarity index considering all samples varied from 0.4 to 0.87, showing a wide range of variation. A dendrogram showed a clear grouping of fish collected from the Laguna de Terminos, Rio Coatzacoalcos and Laguna de Celestun, with discrete subgroups according to region. In contrast, fish from Laguna de Mecoacan were grouped together completely separate from the rest of the fish. Despite the low number of fish from Mecoacan (a high bootstrap support was observed in this group), the results indicated a significant genetic variability in comparison with the other ecosystems included. The differential level of expression of CYP1A and the EROD activity observed among the ecosystems analyzed could be due to the high range of genetic variation, with special emphasis on fish collected in Mecoacan where it is possible to find a subspecies of Ariopsis felis.

  4. Ecotoxicological effects of POPs on ariidae Ariopsis felis (Linnaeus, 1766) from three coastal ecosystems in the Southern Gulf of Mexico and Yucatan Peninsula.

    Science.gov (United States)

    Zapata-Pérez, Omar; Ceja-Moreno, Victor; Olmos, Mónica Roca; Pérez, María Teresa; Río-García, Marcela Del; Yarto, Mario; Mendoza-Cantú, Ania; Ize-Lema, Ana-Irina; Gavilán-García, Arturo; Felipe, Sánchez-Teyer L; Gold-Bouchot, Gerardo

    2007-08-01

    Persistent Organic Pollutants (POPs) are still used for agricultural and disease vector control, as well as for industrial purposes. In the last decades, various studies have shown that fish are sensitive to the toxicological effects of certain POPs, including a large class of endocrine- disrupting chemicals (EDCs). In the present study, the relationship between of POPs and their effects using vitellogenin gene expression as biomarker of effect in hardhead catfish Ariopsis felis (Linnaeus, 1766) from three ecosystems in the Southern Gulf of Mexico and Yucatan Peninsula are discussed. Contaminant results showed that median concentrations of PCBs, HCHs, DDTs and Chlordanes were higher in Laguna de Terminos with respect to Celestun and Dzilam. In the same way, the vitellogenin gene expression was clearly over-expressed in fish collected from Terminos Lagoon. Principal Component Analysis showed that vitellogenin gene expression is related to the concentrations of total DDTs and PCBs, and negatively related to total Drins. Overall, this study represents the first tests exploring changes in molecular diagnostic indicators following exposure of several organic compounds in our country. Vitellogenin gene expressions associated with some endocrine disruptors detected in Terminos Lagoon were measured and we can now report clear changes in fish exposed.

  5. Phylogenetic relationships among five marine Catfish species (Pisces: Ariidae from Mexico Relaciones filogenéticas entre cinco especies de bagres (Pisces: Ariidae de México

    Directory of Open Access Journals (Sweden)

    Guadalupe Tenorio-Colín

    2010-01-01

    Full Text Available The systematics of the marine catfish of the family Ariidae is controversial because at the present time the number of species and genera in the family, or their relationships, remain uncertain. Phylogenetic relationships among five representative species of marine catfish of the family Ariidae from both the Pacific and the Atlantic coasts of Mexico were assessed by the analysis of the variability in 21 alloenzymatic loci, and by the comparison of the electrophoretic patterns of whole muscle proteins. Interspecific genetic divergence levels obtained by both electrophoretic methods showed a clear separation among the genera Cathorops, Bagre and Ariopsis, as well as in the studied species of Cathorops and Ariopsis, with Bagre marinus showing a greater genetic similarity with the Ariopsis group. Finally, our results contribute to the definition of the presence of this species in the coasts of the Gulf of Mexico.La sistemática de los bagres marinos pertenecientes a la familia Ariidae es controversial, porque hasta la actualidad, no se conoce con exactitud el número de especies y géneros existentes en la familia, ni las relaciones que se establecen entre ellos. En el presente trabajo se estudiaron las relaciones filogenéticas entre cinco especies representativas de bagres marinos de la familia Ariidae, de ambas costas del Pacífico y del Atlántico Mexicano. El mencionado análisis se llevó a cabo mediante el análisis de la variabilidad en 21 loci aloenzimáticos y a través de la comparación de patrones electroforéticos de proteínas totales de músculo. Los niveles de divergencia interespecífica obtenidos por ambos métodos electroforéticos mostraron una clara separación entre los géneros Cathorops, Bagre y Ariopsis, así como entre las especies estudiadas de Cathorops y Ariopsis con Bagre marinus, mostrando una mayor similitud genética con el grupo Ariopsis. Los resultados obtenidos en este estudio contribuyen al establecimiento de la

  6. Filogenia de los bagres marinos (Siluriformes: Ariidae del nuevo mundo

    Directory of Open Access Journals (Sweden)

    Betancur Rodríguez Ricardo

    2004-12-01

    Full Text Available La familia Ariidae comprende peces marinos, estuarinos y dulceacuícolas, distribuidos en aguas tropicales y subtropicales de todos los continentes. La nomenclatura específica y genérica en el grupo es caótica y no hay continuidad de su uso en la literatura. En este trabajo se presenta una filogenia robusta para 37-41 especies de ariidos americanos y dos especies del Viejo Mundo, basada en un máximo de 2.922 caracteres del genoma mitocondrial (citocromo b, ATP sintetasa 8 y 6, 12S y 16S y 978 del nuclear (gen activador de la recombinación 2, por reconstrucción con los criterios de máxima parsimonia (MP e inferencia bayesiana (BI. Adicionalmente, se complementó el trabajo morfológico previo incluyendo siete especies y 11 caracteres adicionales, para una matriz final de 26 especies y 56 caracteres. Las secuencias mitocondriales proporcionaron alta resolución a diferentes niveles (subfamiliar, genérico y específico. Aunque BI recuperó en más ocasiones nodos débiles incongruentes con otros conjuntos de datos, mostró mayor sensibilidad que MP a la escasa señal filogenética del locus nuclear, el cual ofreció poca
    resolución. La evidencia morfológica fue congruente con las hipótesis moleculares a nivel subfamiliar, genérico y en gran medida intergenérico; las discrepancias más marcadas entre ambos
    enfoques se dan al interior del género Bagre. Las filogenias obtenidas concuerdan todas con la división de Ariidae en dos subfamilias: Ariinae y el grupo de los Galeichthyinos, el cual requiere
    estatus nomenclatural. Se corroboró la validez, y en gran medida la monofilia de los géneros Ariopsis, Bagre, Cathorops, Galeichthys, Potamarius y Selenaspis (incluyendo a S. proops y S. parkeri. El
    género Arius sensu lato no es monofilético y no debe ser usado para referir a las especies americanas. El linaje 'Arius A' conforma un grupo monofilético anfiamericano al cual debe atribuírsele un nombre gen

  7. Apuntes sobre la reproduccion de algunos Bagres marinos

    NARCIS (Netherlands)

    Luengo, José A.

    1973-01-01

    Mouthbreeding in the male, and modifications of the pelvic fins of the female are recorded for the first time in Selenaspis herzbergii. The pelvic girdle of Selenaspis herzbergii is compared with those of Sciadeichthys proops, Arius spixii, and Bagre marinus. Data are given on eggs and fry in the bu

  8. Rickettsia felis in Ctenocephalides felis from Guatemala and Costa Rica

    Science.gov (United States)

    Troyo, Adriana; Álvarez, Danilo; Taylor, Lizeth; Abdalla, Gabriela; Calderón-Arguedas, Ólger; Zambrano, Maria L.; Dasch, Gregory A.; Lindblade, Kim; Hun, Laya; Eremeeva, Marina E.; Estévez, Alejandra

    2012-01-01

    Rickettsia felis is an emerging human pathogen associated primarily with the cat flea Ctenocephalides felis. In this study, we investigated the presence of Rickettsia felis in C. felis from Guatemala and Costa Rica. Ctenocephalides felis were collected directly from dogs and cats, and analyzed by polymerase chain reaction for Rickettsia-specific fragments of 17-kDa protein, OmpA, and citrate synthase genes. Rickettsia DNA was detected in 64% (55 of 86) and 58% (47 of 81) of flea pools in Guatemala and Costa Rica, respectively. Sequencing of gltA fragments identified R. felis genotype URRWXCal2 in samples from both countries, and genotype Rf2125 in Costa Rica. This is the first report of R. felis in Guatemala and of genotype Rf2125 in Costa Rica. The extensive presence of this pathogen in countries of Central America stresses the need for increased awareness and diagnosis. PMID:22665618

  9. Early Pleistocene lineages of Bagre bagre (Linnaeus, 1766 (Siluriformes: Ariidae, from the Atlantic coast of South America, with insights into the demography and biogeography of the species

    Directory of Open Access Journals (Sweden)

    Wemerson C. da Silva

    Full Text Available ABSTRACT Coastal and marine environments are characterized by a lack of evident physical barriers or geographic isolation, and it may be difficult to understand how divergence can arise and be sustained in marine environments. The identification of 'soft' barriers is a crucial step towards the understanding of gene flow in marine environments. The marine catfishes of the family Ariidae are a demersal group with restricted migratory behavior, no pelagic larval stages, and mechanisms of larval retention, representing a potentially useful model for the understanding of historical processes of allopatric speciation in the marine environment. In the present study, two lineages of the Coco sea catfish, Bagre bagre , were recognized from their complete segregation at both mitochondrial and morphological levels. One lineage is distributed between Venezuela and the northern coast of Brazil, including the semiarid northeast coast, while the second lineage is found on the eastern coast of Brazil, including the humid northeast coast. Based on distribution area, habitats preference, and genetic variability, inferences are made in relation to biogeography and demography of lineages in Atlantic coast of South America.

  10. Efecto de tres tipos de presas vivas en la larvicultura de bagre blanco (Sorubim cuspicaudus)

    OpenAIRE

    Martha Prieto-Guevara; Jhiry Hernández B.; Catalino Gómez R.; Sandra Pardo C.; Victor Atencio-Garcia; Rosa, Priscila V

    2013-01-01

    RESUMENObjetivo. Evaluar el efecto de diferentes presas vivas en la larvicultura de bagre blanco (Sorubim cuspicaudus). Materiales y métodos. Al inicio de la alimentación exógena de Sorubim cuspicaudus, se ofreció zooplancton producido en mesocosmos (T1), zooplancton silvestre (T2) y nauplios de Artemia (T3), en concentración de 10.000 zoop/L, dos veces al día, durante seis días. Se utilizaron 18 acuarios de cinco litros de volumen útil, con densidad de 25 Larvas/L, seis réplicas por tratamie...

  11. Rétrécissement saisonnier des superficies d'eau, variation physico-chimique et production des pêcheries artisanales de Bagré. Centre-Est Burkina-Faso

    Directory of Open Access Journals (Sweden)

    Kabré, AT.

    2000-01-01

    Full Text Available Draw-down of Water Surfaces, Physico-Chemical Variations and the Production of Artisanal Fisheries in the Bagre. East-Center Burkina-Faso. A maping method in respect of the surface of artisanal fisheries using the GPS (Global Positioning System was experimented with in dam waters of the Great Bagre and the Small Bagre. This method has made it possible to estimate an average draw-down of 8.6 % per month in the dry season. This applies more particularly in the small fisheries. The study shows that the water draw-down creates a fluctuation in the depth of the epilimnion and seasonal variations in turbidness and conductivity in the two fisheries. These physico-chemical variations are less significant in the Great Bagre than in the Small Bagre. They are reflected by a relative stability in fish production in the Great Bagre.

  12. Efecto de tres tipos de presas vivas en la larvicultura de bagre blanco (Sorubim cuspicaudus

    Directory of Open Access Journals (Sweden)

    Martha Prieto-Guevara

    2013-11-01

    Full Text Available Objetivo. Evaluar el efecto de diferentes presas vivas en la larvicultura de bagre blanco (Sorubim cuspicaudus. Materiales y métodos. Al inicio de la alimentación exógena de Sorubim cuspicaudus, se ofreció zooplancton producido en mesocosmos (T1, zooplancton silvestre (T2 y nauplios de Artemia (T3, en concentración de 10.000 zoop/L, dos veces al día, durante seis días. Se utilizaron 18 acuarios de cinco litros de volumen útil, con densidad de 25 Larvas/L, seis réplicas por tratamiento en un diseño al azar. Se estimaron la ganancia en peso (Gp y longitud (Gl, tasa de crecimiento específico (G, sobrevivencia (S, resistencia al estrés (Re, mortalidad acumulada (Ma y mortalidad por canibalismo (Mc. Resultados. Las larvas alimentadas con mesocosmos presentaron la mayor sobrevivencia (81.3±15.9%; aunque el mejor crecimiento lo presentaron las larvas alimentadas con zooplancton silvestre (T2 las cuales presentaron la mayor mortalidad (42.0±10.7% y la menor resistencia al estrés (30.0±33.0%. El canibalismo se observó en todos los tratamientos, oscilando entre 4.0 (T2 y 14.3% (T1 sin diferencias significativas entre estos valores (p>0.05. Conclusiones. El uso de zooplancton producido bajo condiciones controladas permitió una alta sobrevivencia, adecuado desempeño y resistencia de las larvas, perfilándose como alternativa viable en la primera alimentación de bagre blanco.

  13. Rickettsia felis, an emerging flea-transmitted human pathogen

    Directory of Open Access Journals (Sweden)

    Stephen Graves

    2011-07-01

    Full Text Available Rickettsia felis was first recognised two decades ago and has now been described as endemic to all continents except Antarctica. The rickettsiosis caused by R. felis is known as flea-borne spotted fever or cat-flea typhus. The large number of arthropod species found to harbour R. felis and that may act as potential vectors support the view that it is a pan-global microbe. The main arthropod reservoir and vector is the cat flea, Ctenocephalides felis, yet more than 20 other species of fleas, ticks, and mites species have been reported to harbour R. felis. Few bacterial pathogens of humans have been found associated with such a diverse range of invertebrates. With the projected increase in global temperature over the next century, there is concern that changes to the ecology and distribution of R. felis vectors may adversely impact public health.

  14. Biomarcadores histológicos em duas espécies de bagres estuarinos da Costa Maranhense, Brasil

    Directory of Open Access Journals (Sweden)

    D.B.P.Sousa

    2013-04-01

    Full Text Available Neste estudo objetivou-se validar lesões branquiais como biomarcadores em peixes de importância econômica (Sciades herzbergii e Bagre bagre capturados pela pesca artesanal em dois locais diferenciados da Baía de São Marcos, MA, a fim de selecionar um táxon como bioindicador capaz de ser utilizado em programas de biomonitoramento. O primeiro ponto (S1 foi utilizado como uma área de referência - Ilha dos Caranguejos -, e o segundo ponto (S2 foi considerado potencialmente impactado - Complexo Portuário de São Luís, MA. Encontraram-se várias alterações histológicas para as duas espécies em S2, destacando-se: estreitamento lamelar, teleangectasia, fusão e descolamento do epitélio da lamela secundária. Lesões branquiais em B. bagre coletados em S1 também foram observadas, não sendo possível diferenciar os indivíduos das duas áreas analisadas. Os dados indicam que S. herzbergii é um bioindicador mais apropriado para análise de biomarcadores de contaminação aquática.

  15. In vitro efficacy of essential oils and extracts of Schinus molle L. against Ctenocephalides felis felis.

    Science.gov (United States)

    Batista, Lilian C De S O; Cid, Yara P; De Almeida, Ana Paula; Prudêncio, Edlene R; Riger, Cristiano J; De Souza, Marco A A; Coumendouros, Katherine; Chaves, Douglas S A

    2016-04-01

    Extracts and essential oils from plants are important natural sources of pesticides. These compounds are considered an alternative to control ectoparasites of veterinary importance. Schinus molle, an endemic species of Brazil, produces a high level of essential oil and several other compounds. The aim of this work was to determinate the chemical composition of extracts and essential oils of S. molle and further to evaluate the activity against eggs and adults of Ctenocephalides felis felis, a predominant flea that infests dogs and cats in Brazil. In an in vitro assay, the non-polar (n-hexane) extract showed 100% efficacy (800 µg cm(-2); LD50 = 524·80 µg cm(-2)) at 24 and 48 h. Its major compound was lupenone (50·25%). Essential oils from fruits and leaves were evaluated, and had 100% efficacy against adult fleas at 800 µg cm(-2) (LD50 = 353·95 µg cm(-2)) and at 50 µg cm(-2) (LD50 = 12·02 µg cm(-2)), respectively. On the other hand, the essential oil from fruits and leaves was not active against flea eggs. This is the first study that reports the insecticidal effects of essential oils and extracts obtained from Schinus molle against Ctenocephalides felis felis.

  16. El bagre de canal (Ictalurus punctatus Rafinesque, 1818: estado actual y problemática en México

    Directory of Open Access Journals (Sweden)

    Ana Laura Lara-Rivera

    2015-07-01

    Full Text Available El bagre de canal (Ictalurus punctatus es uno de los peces endémicos de Norte América con mayor potencial para la acuicultura. A pesar de esto, los datos sobre su distribución actual son escasos y se desconocen muchos aspectos de su dinámica y desempeño productivo. En el presente estudio se reportan los resultados de una búsqueda bibliográfica y análisis de campo que permiten describir la situación actual del bagre de canal con respecto a su distribución geográfica histórica y actual, así como de la problemática que enfrentan los sistemas productivos de esta especie en México. Se identificó la falta de información e indicadores que permitan conocer la extensión de su hábitat natural, movilización o potencial productivo. La calidad y disponibilidad del agua figuran entre las principales problemáticas de la industria; el alimento supone el mayor gasto para los productores y existe gran heterogeneidad de manejo tanto en granjas reproductoras como de engorde, gran parte de la dinámica productiva sigue un modelo de ensayo y error. Se requiere optimizar y homologar los procesos del sistema productivo de bagre de canal, así como caracterizar correctamente las poblaciones domésticas y silvestres para aprovechar al máximo su potencial.

  17. Integrated morphological and molecular identification of cat fleas (Ctenocephalides felis) and dog fleas (Ctenocephalides canis) vectoring Rickettsia felis in central Europe.

    Science.gov (United States)

    Lawrence, Andrea L; Hii, Sze-Fui; Jirsová, Dagmar; Panáková, Lucia; Ionică, Angela M; Gilchrist, Katrina; Modrý, David; Mihalca, Andrei D; Webb, Cameron E; Traub, Rebecca J; Šlapeta, Jan

    2015-06-15

    Fleas of the genus Ctenocephalides are the most common ectoparasites infesting dogs and cats world-wide. The species Ctenocephalides felis and Ctenocephalides canis are competent vectors for zoonotic pathogens such as Rickettsia felis and Bartonella spp. Improved knowledge on the diversity and phylogenetics of fleas is important for understanding flea-borne pathogen transmission cycles. Fleas infesting privately owned dogs and cats from the Czech Republic (n=97) and Romania (n=66) were subjected to morphological and molecular identification and phylogenetic analysis. There were a total of 59 (60.82%) cat fleas (Ctenocephalides felis felis), 30 (30.93%) dog fleas (Ctenocephalides canis), 7 (7.22%) European chicken fleas (Ceratophyllus gallinae) and 1 (1.03%) northern rat flea (Nosopsyllus fasciatus) collected in the Czech Republic. Both C. canis and C. felis felis were identified in Romania. Mitochondrial DNA sequencing at the cox1 gene on a cohort of 40 fleas revealed the cosmopolitan C. felis felis clade represented by cox1 haplotype 1 is present in the Czech Republic. A new C. felis felis clade from both the Czech Republic and Romania is also reported. A high proportion of C. canis was observed from dogs and cats in the current study and phylogeny revealed that C. canis forms a sister clade to the oriental cat flea Ctenocephalides orientis (syn. C. felis orientis). Out of 33 fleas tested, representing C. felis felis, C. canis and Ce. gallinae, 7 (21.2%) were positive for R. felis using diagnostic real-time PCR targeting the gltA gene and a conventional PCR targeting the ompB gene. No samples tested positive for Bartonella spp. using a diagnostic real-time PCR assay targeting ssrA gene. This study confirms high genetic diversity of C. felis felis globally and serves as a foundation to understand the implication for zoonotic disease carriage and transmission by the flea genus Ctenocephalides.

  18. Common epidemiology of Rickettsia felis infection and malaria, Africa.

    Science.gov (United States)

    Mediannikov, Oleg; Socolovschi, Cristina; Edouard, Sophie; Fenollar, Florence; Mouffok, Nadjet; Bassene, Hubert; Diatta, Georges; Tall, Adama; Niangaly, Hamidou; Doumbo, Ogobara; Lekana-Douki, Jean Bernard; Znazen, Abir; Sarih, M'hammed; Ratmanov, Pavel; Richet, Herve; Ndiath, Mamadou O; Sokhna, Cheikh; Parola, Philippe; Raoult, Didier

    2013-11-01

    This study aimed to compare the epidemiology of Rickettsia felis infection and malaria in France, North Africa, and sub-Saharan Africa and to identify a common vector. Blood specimens from 3,122 febrile patients and from 500 nonfebrile persons were analyzed for R. felis and Plasmodium spp. We observed a significant linear trend (pAfrica also protects against rickettsioses; thus, empirical treatment strategies for febrile illness for travelers and residents in sub-Saharan Africa may require reevaluation.

  19. An update on the detection and treatment of Rickettsia felis

    Directory of Open Access Journals (Sweden)

    Hun L

    2012-06-01

    Full Text Available Laya Hun, Adriana TroyoCentro de Investigación en Enfermedades Tropicales, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa RicaAbstract: Rickettsia felis was described as a human pathogen almost two decades ago, and human infection is currently reported in 18 countries in all continents. The distribution of this species is worldwide, determined by the presence of the main arthropod vector, Ctenocephalides felis (Bouché. The list of symptoms, which includes fever, headache, myalgia, and rash, keeps increasing as new cases with unexpected symptoms are described. Moreover, the clinical presentation of R. felis infection can be easily confused with many tropical and nontropical diseases, as well as other rickettsial infections. Although specific laboratory diagnosis and treatment for this flea-borne rickettsiosis are detailed in the scientific literature, it is possible that most human cases are not being diagnosed properly. Furthermore, since the cat flea infests different common domestic animals, contact with humans may be more frequent than reported. In this review, we provide an update on methods for specific detection of human infection by R. felis described in the literature, as well as the treatment prescribed to the patients. Considering advances in molecular detection tools, as well as options for as-yet-unreported isolation of R. felis from patients in cell culture, increased diagnosis and characterization of this emerging pathogen is warranted.Keywords: Rickettsia felis, human cases, laboratory diagnosis, treatment

  20. Molecular Detection of Rickettsia felis in Humans, Cats, and Cat Fleas in Bangladesh, 2013-2014.

    Science.gov (United States)

    Ahmed, Rajib; Paul, Shyamal Kumar; Hossain, Muhammad Akram; Ahmed, Salma; Mahmud, Muhammad Chand; Nasreen, Syeda Anjuman; Ferdouse, Faria; Sharmi, Rumana Hasan; Ahamed, Farid; Ghosh, Souvik; Urushibara, Noriko; Aung, Meiji Soe; Kobayashi, Nobumichi

    2016-05-01

    High prevalence of Rickettsia felis in patients with fever of unknown origin was revealed in the north-central Bangladesh from 2012 to 2013. Subsequently, in this study, prevalence of R. felis in cats and cat fleas (Ctenocephalides felis), together with febrile patients, was studied by PCR detection of 17 kDa antigen gene and DNA sequencing. R. felis was detected in 28% (28/100) and 21% (14/68) of cat blood and cat flea samples, respectively, whereas 42% (21/50) of patients were positive for R. felis. R. felis-positive cat fleas were detected at significantly higher rate on R. felis-positive cats. The results suggested a potential role of cats and cat fleas for transmission of R. felis to humans in Bangladesh.

  1. HISTOLOGÍA Y MORFOMETRÍA DEL SISTEMA DIGESTIVO DEL SILÚRIDO BAGRE TIGRITO (Pimelodus pictus)

    OpenAIRE

    2007-01-01

    El bagre tigrito Pimelodus pictus es un pez ornamental con gran aceptación en el mercado acuarista internacional, sin embargo, se desconocen muchos aspectos de la biología básica de esta especie. Por tal motivo, se realizó un estudio histológico y morfométrico del sistema digestivo a Àn de contribuir al diseño de futuras dietas. Se sacriÀcaron 7 individuos adultos de P. pictus de 10 ± 0,5 cm de longitud total con una sobredosis de MS-222 (0,5 g/l). Los especímenes se Àjaron en formaldehído al...

  2. Monitoring water turbidity and surface suspended sediment concentration of the Bagre Reservoir (Burkina Faso) using MODIS and field reflectance data

    Science.gov (United States)

    Robert, Elodie; Grippa, Manuela; Kergoat, Laurent; Pinet, Sylvain; Gal, Laetitia; Cochonneau, Gérard; Martinez, Jean-Michel

    2016-10-01

    Monitoring turbidity and Surface Suspended Sediment Concentration (SSSC) of inland waters is essential to address several important issues: erosion, sediment transport and deposition throughout watersheds, reservoir siltation, water pollution, human health risks, etc. This is especially important in regions with limited conventional monitoring capacities such as West Africa. In this study, we explore the use of Moderate Resolution Imaging Spectroradiometer data (MODIS, MOD09Q1 and MYD09Q1 products, red (R) and near infrared (NIR) bands) to monitor turbidity and SSSC for the Bagre Reservoir in Burkina Faso. High values ​​of these parameters associated with high spatial and temporal variability potentially challenge the methodologies developed so far for less turbid waters. Field measurements (turbidity, SSSC, radiometry) are used to evaluate different radiometric indices. The NIR/R ratio is found to be the most suited to retrieve SSSC and turbidity for both in-situ spectoradiometer measurements and satellite reflectance from MODIS. The spatio temporal variability of MODIS NIR/R together with rainfall estimated by the Tropical Rainforest Measuring Mission (TRMM) and altimetry data from Jason-2 is analyzed over the Bagre Reservoir for the 2000-2015 period. It is found that rain events of the early rainy season (February-March) through mid-rainy season (August) are decisive in triggering turbidity increase. Sediment transport is observed in the reservoir from upstream to downstream between June and September. Furthermore, a significant increase of 19% in turbidity values is observed between 2000 and 2015, mainly for the July to December period. It is especially well marked for August, with the central and downstream areas showing the largest increase. The most probable hypothesis to explain this evolution is a change in land use, and particularly an increase in the amount of bare soils, which enhances particle transport by runoff.

  3. Optical properties of infrared FELs from the FELI Facility II

    Energy Technology Data Exchange (ETDEWEB)

    Saeki, K.; Okuma, S.; Oshita, E. [Free Electron Laser Institute, Osaka (Japan)] [and others

    1995-12-31

    The FELI Facility II has succeeded in infrared FEL oscillation at 1.91 {mu} m using a 68-MeV, 40-A electron beam from the FELI S-band linac in February 27, 1995. The FELI Facility II is composed of a 3-m vertical type undulator ({lambda}u=3.8cm, N=78, Km a x=1.4, gap length {ge}20mm) and a 6.72-m optical cavity. It can cover the wavelength range of 1-5{mu}m. The FELs can be delivered from the optical cavity to the diagnostics room through a 40-m evacuated optical pipeline. Wavelength and cavity length dependences of optical properties such as peak power, average power, spectrum width, FEL macropulse, FEL transverse profile are reported.

  4. Molecular evidence of Rickettsia felis infection in dogs from northern territory, Australia

    Directory of Open Access Journals (Sweden)

    Rees Robert L

    2011-10-01

    Full Text Available Abstract The prevalence of spotted fever group rickettsial infection in dogs from a remote indigenous community in the Northern Territory (NT was determined using molecular tools. Blood samples collected from 130 dogs in the community of Maningrida were subjected to a spotted fever group (SFG-specific PCR targeting the ompB gene followed by a Rickettsia felis-specific PCR targeting the gltA gene of R. felis. Rickettsia felis ompB and gltA genes were amplified from the blood of 3 dogs. This study is the first report of R. felis infection in indigenous community dogs in NT.

  5. Outbreaks of Rickettsia felis in Kenya and Senegal, 2010

    Centers for Disease Control (CDC) Podcasts

    2010-06-09

    This podcast describes the outbreak of Rickettsia felis in Kenya between August 2006 and June 2008, and in rural Senegal from November 2008 through July 2009. CDC infectious disease pathologist Dr. Chris Paddock discusses what researchers learned about this flea-borne disease and how to prevent infection.  Created: 6/9/2010 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 6/24/2010.

  6. Antimalaric antiobodies in school children in El Bagre, Colombia Detección de anticuerpos antimaláricos en un grupo de escolares del municipio de El Bagre, Antioquia

    Directory of Open Access Journals (Sweden)

    Isabel Polanco

    1998-01-01

    Full Text Available A cases and controls study was carried out in 1996 in El Bagre, Colombia, endemic zone for malaria, in order to determine the relationship between nutritional situation and immune response to malaria in 51 children with malaria and 49 without it; IgG and IgM antibodies were determined by means of indirect inmunofluorescence .against P. falciparum antigens (Strain FCB2. Nutritional situation was evaluated according to USA National Center for Health Statistics. No child was found positive for IGM antibodies; concerning IgG response, it was positive in 29 (56.9% malaric children but only in 4 (8.2% of the non-malaric ones (p<0.00001 . Difference was also signjficant (p<0.00001 for malarja antecedents in the previous year (70.6% in malaric children vs 10.2% jn the nonmalaric ones. Of the 29 malaric seropositive children 20 (69% were malnourished (p<0.01 . En 1996, en el municipio de El Bagre (Antioquia-Colombia, zona endémica para malaria y con altos niveles de desnutrición, se realizó un estudio de casos y controles con el objeto de determinar la relación entre el estado nutricional y la respuesta inmune humoral de niños con y sin malaria. Se trabajó con un grupo de 100 niños entre 4 y 9 años de edad, 51 con malaria y 49 sin ella, al cual se le determinaron los niveles de anticuerpos antimaláricos IgG e IgM por la técnica de inmunofluorescencia indirecta (IFI, empleando antigenos de P. falciparum cepa FCB 2 mantenida en cultivo continuo. El estado nutricional se evaluó por comparación de las medidas antropométricas con la referencia de crecimiento del National Center for Health Statistics (NCHS de los Estados Unidos. Los resultados mostraron diferencias en la tasa de positividad de anticuerpos IgG entre los niños maláricos y los no maláricos. En efecto: Veintinueve niños maláricos (56.9% y sólo 4 de los no maláricos (8.2% tenían dichos anticuerpos (P<0.00001 ; la diferencia también fue significativa para el antecedente de

  7. Life cycle of Cystoisospora felis (Coccidia: Apicomplexa) in cats and mice

    Science.gov (United States)

    Cystoisospora felis is a ubiquitous apicomplexan protozoon of cats. The endogenous development of C. felis was studied in cats after feeding them infected mice. For this, 5 newborn cats were killed at 24, 48, 72, 96, and 120 h after having been fed mesenteric lymph nodes and spleens of mice that wer...

  8. Cat fleas (Ctenocephalides felis) from cats and dogs in New Zealand: Molecular characterisation, presence of Rickettsia felis and Bartonella clarridgeiae and comparison with Australia.

    Science.gov (United States)

    Chandra, Shona; Forsyth, Maureen; Lawrence, Andrea L; Emery, David; Šlapeta, Jan

    2017-01-30

    The cat flea (Ctenocephalides felis) is the most common flea species parasitising both domestic cats and dogs globally. Fleas are known vectors of zoonotic pathogens such as vector borne Rickettsia and Bartonella. This study compared cat fleas from domestic cats and dogs in New Zealand's North and South Islands to Australian cat fleas, using the mitochondrial DNA (mtDNA) marker, cytochrome c oxidase subunit I and II (cox1, cox2). We assessed the prevalence of Rickettsia and Bartonella using genus specific multiplexed real-time PCR assays. Morphological identification confirmed that the cat flea (C. felis) is the most common flea in New Zealand. The examined fleas (n=43) at cox1 locus revealed six closely related C. felis haplotypes (inter-haplotype distance 1.1%) across New Zealand. The New Zealand C. felis haplotypes were identical or near identical with haplotypes from southern Australia demonstrating common dispersal of haplotype lineage across both the geographical (Tasman Sea) and climate scale. New Zealand cat fleas carried Rickettsia felis (5.3%) and Bartonella clarridgeiae (18.4%). To understand the capability of C. felis to vector zoonotic pathogens, we determined flea cox1 and cox2 haplotype diversity with the tandem multiplexed real-time PCR and sequencing for Bartonella and Rickettsia. This enabled us to demonstrate highly similar cat fleas on cat and dog populations across Australia and New Zealand.

  9. Identification of Ctenocephalides felis fleas as a host of Rickettsia felis, the agent of a spotted fever rickettsiosis in Yucatań, Mexico.

    Science.gov (United States)

    Zavala-Velázquez, J E; Zavala-Castro, J E; Vado-Solís, I; Ruiz-Sosa, J A; Moron, C G; Bouyer, D H; Walker, D H

    2002-01-01

    In search for the vector of the recently recognized spotted fever rickettsiosis of the Yucatán, ticks, fleas, and lice were collected from vegetation and dogs in localities where seropositive persons had been found. The arthropods were examined by polymerase chain reaction (PCR) using primers for the genus-specific 17-kDa protein gene followed by restriction fragment length polymorphism (RFLP) and DNA sequencing. Eleven (20%) of 54 pools of Ctenocephalides felis fleas contained DNA of Rickettsia felis. None of 219 Amblyomma cajennense, 474 Rhiphicephalus sanguineus, 258 Boophilus sp. ticks, and 33 Poliplax species lice contained DNA of Rickettsia. The identity of the rickettsial DNA was confirmed as R. felis by PCR/RFLP for the citrate synthase and outer membrane protein A genes and by DNA sequencing. The results indicate that the host of R. felis in Yucatán is C. felis and suggest that the spotted fever rickettsiosis that has infected >5% of the population of the Yucatán and can present as a dengue-like illness is likely to be caused by R. felis.

  10. Multilocus variable-number tandem-repeat analysis scheme for chlamydia felis genotyping: comparison with multilocus sequence typing.

    Science.gov (United States)

    Laroucau, Karine; Di Francesco, Antonietta; Vorimore, Fabien; Thierry, Simon; Pingret, Jean Luc; Bertin, Claire; Willems, Hermann; Bölske, Goran; Harley, Ross

    2012-06-01

    Chlamydia felis is an important ocular pathogen in cats worldwide. A multilocus variable-number tandem-repeat analysis (MLVA) system for the detection of tandem repeats across the whole genome of C. felis strain Fe/C-56 was developed. Nine selected genetic loci were tested by MLVA in 17 C. felis isolates, including the C. felis Baker vaccine strain, and 122 clinical samples from different geographic origins. Analysis of the results identified 25 distinct C. felis MLVA patterns. In parallel, a recently described multilocus sequence typing scheme for the typing of Chlamydia was applied to 13 clinical samples with 12 different C. felis MLVA patterns. Rare sequence differences were observed. Thus, the newly developed MLVA system provides a highly sensitive high-resolution test for the differentiation of C. felis isolates from different origins that is suitable for molecular epidemiological studies.

  11. Tsunami induced transportation of the coastal marine sediments to distant onshore regions: Some indications from foraminiferal and microbenthic studies of new Wandoor region (Andaman & Nicobar)

    Digital Repository Service at National Institute of Oceanography (India)

    Khare, N.; Chaturvedi, S.K.; Ingole, B.S.

    (Ariopsis felis) and their potential to record low latitude climate change. Paleoceanography, Paleoclimatology and Paleoecology, 28, 179-191 (2005) 2. Harris P.T., Marine geology and sedimentology of the Australian continental shelf. In: Leon P. Zann (Ed...

  12. Variación estacional de las características seminales del bagre rayado Pseudoplatystoma metaense (Telostei, pimelodidae

    Directory of Open Access Journals (Sweden)

    Juan Ramirez-Merlano

    2011-04-01

    Full Text Available Objetivo. Determinar la variación de la calidad, el contenido iónico y la osmolaridad del plasma seminal de Pseudoplatystoma metaense durante la estación reproductiva. Materiales y métodos. Se utilizaron machos sexualmente maduros durante un periodo reproductivo (marzo a agosto. La espermiación fue inducida con Extracto de Hipófisis de Carpa (4 mg/kg. Se evaluó el volumen (mL, movilidad masal (%, tiempo de activación (sg, espermatocrito (%; concentración espermática (106 sptz/µL y viabilidad (%. También fue determinada la movilidad (% y velocidad individual por medio de un Sistema de Análisis Espermático Asistido por Computador (CASA. La osmolaridad (mOsm/Kg y la concentración de iones (Na+, Cl+, K+ y Mg2+ y glucosa fue determinada en plasma seminal obtenido por centrifugación del semen a 14.000 g. La concentración de iones y glucosa fue establecida a través de un sistema de reflectancia. Resultados. El semen de Pseudoplatystoma metaense presentó una movilidad masal mayor al 90%, con el menor valor de movilidad progresiva lineal rápida para el mes de abril (34.9±9.0% y en general valores de espermatozoides inmóviles menores al 10%. El plasma seminal mostró una osmolaridad de 259.3± 3.5 mOsm/Kg, con una concentración promedio de glucosa e iones Na+, Cl+, K+ y Mg2+ de 142.7±2.8, 118±2.2, 4.8±0.4 y 0.33±0.0 mmol/L, respectivamente, durante la época reproductiva. Conclusiones. La calidad seminal de bagre rayado no presentó variaciones en la estación reproductiva mostrando una alta calidad para los procesos de fecundación.

  13. HISTOLOGÍA Y MORFOMETRÍA DEL SISTEMA DIGESTIVO DEL SILÚRIDO BAGRE TIGRITO (Pimelodus pictus

    Directory of Open Access Journals (Sweden)

    CM Olaya

    2007-01-01

    Full Text Available El bagre tigrito Pimelodus pictus es un pez ornamental con gran aceptación en el mercado acuarista internacional, sin embargo, se desconocen muchos aspectos de la biología básica de esta especie. Por tal motivo, se realizó un estudio histológico y morfométrico del sistema digestivo a Àn de contribuir al diseño de futuras dietas. Se sacriÀcaron 7 individuos adultos de P. pictus de 10 ± 0,5 cm de longitud total con una sobredosis de MS-222 (0,5 g/l. Los especímenes se Àjaron en formaldehído al 4%, durante cinco días a 4 °C. Luego se siguió el procedimiento para técnica en paraÀna. Se realizaron cortes de 5 μm de espesor y se colo-rearon con H&E. El sistema digestivo presentó cuatro capas: mucosa, submucosa, muscular y serosa. En el esófago la mucosa estaba compuesta por epitelio plano estratiÀcado con gran número de células caliciformes, y una submucosa de tejido conectivo laxo, seguida por músculo estriado esquelético con dos orientaciones (circular y longitudinal. En todos los órganos evaluados la capa serosa fue muy delgada. En el estómago se identiÀcaron dos regiones, la pilórica y la cardíaca, encontrándose glándulas gástricas en esta última, ambas regiones presentaban una mucosa con epitelio cilíndrico simple, una submucosa de tejido conectivo laxo, y una capa de músculo liso con dos orientaciones (circular y longitudinal así mismo, este órgano fue el que exhibió el mayor espesor en la capa mucosa y muscular. La histología de la mucosa, la submucosa y la capa muscular del intestino fue similar a lo presentado por el estómago, aunque morfométricamente esta región fue la que exhibió los menores valores en todas las capas evaluadas. El estómago bien deÀnido y el intestino con pocos ciegos pilóricos hace suponer que P. pictus es de hábitos omnívoros con preferencia de los alimentos de origen animal.

  14. Presencia de Arius grandicassis en el Caribe colombiano, incluyendo una clave para la identificación de los peces de la familia Ariidae en el área

    OpenAIRE

    Mejía-Ladino, L.M.; Betancur-R., R.; Acero-P., A.; Zarza-G-, E.

    2002-01-01

    The family Ariidac in the Colombian Caribbean coast includes five well known species (Ariopsis bonillai, Arius props, Bagre bagre, B. marinus and Selenaspis herzbergii) and at least two undescribed of the genus Cathorops. In this paper we report by the first time the presence of Arius gandicassis Valenciennes, 1840 in the region, based on three specimens of 342 - 495 mm total length captured in offshore waters at San Lorenzo de Camarones (La Guajira), widening in this way the species distribu...

  15. Detection of Rickettsia felis in Wild Mammals from Three Municipalities in Yucatan, Mexico.

    Science.gov (United States)

    Panti-May, Jesús Alonso; Torres-Castro, Marco; Hernández-Betancourt, Silvia; Dzul-Rosado, Karla; Zavala-Castro, Jorge; López-Avila, Karina; Tello-Martín, Raúl

    2015-09-01

    The aim of this study was to provide information of the occurrence of Rickettsia felis in wild mammals from three municipalities in Yucatan, Mexico. The reactivity of rodent serum to Rickettsia antigens was detected in 80.9% (17 of 21) samples using immunofluorescence assay. Polymerase chain reaction identified rickettsial DNA in spleens of 43.5% (10 of 23) rodents and 57.1% (4 of 7) opossums. The identification of the rickettsial DNA was confirmed as R. felis by restriction fragment length polymorphism and DNA sequencing. This study comprises the first report of R. felis detection in wild mammals in Yucatan.

  16. Biomarcadores histológicos em duas espécies de bagres estuarinos da Costa Maranhense, Brasil Histology biomarkers in two estuarine catfish species from the Maranhense Coast, Brazil

    Directory of Open Access Journals (Sweden)

    D.B.P.Sousa

    2013-04-01

    Full Text Available Neste estudo objetivou-se validar lesões branquiais como biomarcadores em peixes de importância econômica (Sciades herzbergii e Bagre bagre capturados pela pesca artesanal em dois locais diferenciados da Baía de São Marcos, MA, a fim de selecionar um táxon como bioindicador capaz de ser utilizado em programas de biomonitoramento. O primeiro ponto (S1 foi utilizado como uma área de referência - Ilha dos Caranguejos -, e o segundo ponto (S2 foi considerado potencialmente impactado - Complexo Portuário de São Luís, MA. Encontraram-se várias alterações histológicas para as duas espécies em S2, destacando-se: estreitamento lamelar, teleangectasia, fusão e descolamento do epitélio da lamela secundária. Lesões branquiais em B. bagre coletados em S1 também foram observadas, não sendo possível diferenciar os indivíduos das duas áreas analisadas. Os dados indicam que S. herzbergii é um bioindicador mais apropriado para análise de biomarcadores de contaminação aquática.This study aimed to validate branchial lesions as a biomarker in fish (Sciades herzbergii and Bagre bagre caught by traditional fishing in two different places at São Marcos Bay, MA, in order to select a bioindicator taxon which can be used in biomonitoring programs. The first point (S1 was used as a reference area - Caranguejos (Crab Island and the second point (S2 was considered potentially impacted - Port Complex of São Luis, MA. Several histological changes were found for both species in S2, including: narrowing lamellar, teleangectasy, fusion and separation of secondary lamellar epithelium. Gill lesions in B. bagre collected were also observed in S1, it is not possible to differentiate between individuals of the two areas analyzed. The data indicate that S. herzbergii is more appropriate as a bioindicator for analysis of biomarkers of aquatic contamination.

  17. Case report: pulicosis por Ctenocephalides felis felis en ovinos y caprinos en la sabana de Bogotá, Colombia

    Directory of Open Access Journals (Sweden)

    Efraín Benavides Ortiz

    2010-06-01

    Full Text Available In Colombia the rearing of hair sheep and goats are expanding in various regions for being an alternative for meat and milk production at competitive prices due to their adaptability and easiness to digest rough fodder. Among the ectoparasites that affect small ruminants traditionally are recognized the lice and the sheep keds Melophagus ovinus (Díptera: Hippoboscidae, however fleas are not included. Here the occurrence of the common cat flea Ctenocephalides felis felis (Díptera: Siphonaptera affecting sheep and goats in a farm at the Sabana de Bogotá are described an so there was performed an epidemiological and parasitological evaluation. The barn maintained animals in rotational grazing at an approximated stocking rate of 25 head/ha, receiving additional supplements of hay and silage. The presence of the flea was confirmed in sheep and goats, young and adult, as well as in dogs. Diverse degrees of anemia were evidenced but the association between flea infestation and anemia, or the presence of other anemia producing agents could not be studied. In the farm synthetic parasiticides are not used, extracts of Ruda (Ruta graveolens are administered to mitigate parasitosis, without major efficacy. Sheep and goat breeders in the tropics should consider flea infestation as an agent causing adverse animal welfare situations in their farms. Control should start from the knowledge of the life cycle of the flea, trying to interrupt it.

  18. In vitro fertilization and sperm cryopreservation in the black-footed cat (Felis nigripes) and sand cat (Felis margarita).

    Science.gov (United States)

    Herrick, J R; Campbell, M; Levens, G; Moore, T; Benson, K; D'Agostino, J; West, G; Okeson, D M; Coke, R; Portacio, S C; Leiske, K; Kreider, C; Polumbo, P J; Swanson, W F

    2010-03-01

    Studies of in vitro fertilization (IVF) and sperm cryopreservation have been conducted in several small cat species, but virtually no data exist for black-footed cats (Felis nigripes) (BFCs) or sand cats (Felis margarita) (SCs). The objectives of this study were 1) to compare in vitro motility and acrosome status of fresh and cryopreserved (frozen in pellets on dry ice or in straws in liquid nitrogen vapor) BFC and SC spermatozoa cultured in feline-optimized culture medium (FOCM) or Ham F-10, 2) to assess ovarian responsiveness in BFCs and SCs following exogenous gonadotropin treatment and laparoscopic oocyte recovery, and 3) to evaluate the fertility of fresh and frozen-thawed spermatozoa from both species using homologous and heterologous (domestic cat oocytes) IVF in the two culture media. Motility and acrosomal integrity of fresh and frozen-thawed spermatozoa from BFCs and SCs were similar (P > 0.05) in both media during 6 h of culture. Although effects were more pronounced in SCs, cryopreservation in straws was superior (P 80% of recovered oocytes were of optimal (grade 1) quality. The BFC and SC spermatozoa fertilized 60.0%-79.4% of homologous and 37.7%-42.7% of heterologous oocytes in both culture media, with increased (P < 0.05) cleavage of homologous (SC) and heterologous (BFC and SC) oocytes in FOCM. These results provide the first information to date on the gamete biology of two imperiled cat species and further our capacity to apply reproductive technologies for their conservation.

  19. Efecto antioxidante y antimicrobiano de sales de ácidos orgánicos y extractos naturales en filetes de bagre dorado (Brachyplatystoma rousseauxii refrigerados

    Directory of Open Access Journals (Sweden)

    José Vicente Pacheco Guerrero

    2011-01-01

    Full Text Available En el presente trabajo fueron investigados los efectos del lactato de sodio, acetato de sodio, romero y ajo en soluciones acuosas a 2,5 % sobre la calidad microbiológica y oxidación lipídica en rebanadas de bagre dorado (Brachyplatystoma rousseauxii almacenadas a 4 ºC. Los resultados mostraron que estas soluciones fueron eficaces (p acetato de sodio > lactato de sodio > ajo. La vida útil de los productos tratados fue, al menos, de 15 días. Por lo tanto, el acetato de sodio, lactato de sodio, romero y ajo pueden ser utilizados como preservativos seguros para el pescado almacenado bajo refrigeración.

  20. Efecto antioxidante y antimicrobiano de sales de ácidos orgánicos y extractos naturales en filetes de bagre dorado (Brachyplatystoma rousseauxii refrigerados

    Directory of Open Access Journals (Sweden)

    José Vicente Pacheco Guerrero

    2011-06-01

    Full Text Available En el presente trabajo fueron investigados los efectos del lactato de sodio, acetato de sodio, romero y ajo en soluciones acuosas a 2,5 % sobre la calidad microbiológica y oxidación lipídica en rebanadas de bagre dorado (Brachyplatystoma rousseauxii almacenadas a 4 ºC. Los resultados mostraron que estas soluciones fueron eficaces (p acetato de sodio > lactato de sodio > ajo. La vida útil de los productos tratados fue, al menos, de 15 días. Por lo tanto, el acetato de sodio, lactato de sodio, romero y ajo pueden ser utilizados como preservativos seguros para el pescado almacenado bajo refrigeración.

  1. Peptic ulcer disease associated with Helicobacter felis in a dog owner.

    Science.gov (United States)

    De Bock, Manuelle; Van den Bulck, Kathleen; Hellemans, Ann; Daminet, Sylvie; Coche, Jean-Charles; Debongnie, Jean-Claude; Decostere, Annemie; Haesebrouck, Freddy; Ducatelle, Richard

    2007-01-01

    The aim of this study was to investigate the identity of the Helicobacter heilmannii-like bacteria found in the stomach of a human patient suffering from stomach ulcers and her asymptomatic pet dog. An elderly woman was referred for gastroscopy because of right hypochondrial pain, nausea, anorexia and vomiting. Gastric ulcers were observed and histology revealed the presence of multiple H. heilmannii-like bacteria. Multiplex polymerase chain reaction (PCR) identified the bacteria as H. felis. Her pet dog was also examined gastroscopically. Only mild gastric lesions were found but PCR showed the presence of H. felis as well as H. bizzozeronii and Candidatus H. heilmannii. This report associates H. felis infection in humans with severe gastric ulceration. Moreover, the suggestion can be made that the patient contracted H. felis from her dog.

  2. Clusters in social behaviour of female domestic cats (Felis silvestris catus) living in confinement

    NARCIS (Netherlands)

    vandenBos, R; de Vries, Han

    1996-01-01

    Associations between different agonistic and affiliative behavioural patterns of female domestic cats (Felis silvestris catus) were studied. In three groups of intact cats living in confinement frequencies of fourteen agonistic and affiliative behavioural patterns were recorded. The technique of fac

  3. Ctenocephalides felis an in vitro potential vector for five Bartonella species.

    Science.gov (United States)

    Bouhsira, Emilie; Ferrandez, Yann; Liu, MaFeng; Franc, Michel; Boulouis, Henri-Jean; Biville, Francis

    2013-03-01

    The blood-sucking arthropod Ctenocephalides felis has been confirmed as a vector for Bartonella henselae and is a suspected vector for Bartonella clarridgeiae, Bartonella quintana and Bartonella koehlerae in Bartonella transmission to mammals. To understand the absence of other Bartonella species in the cat flea, we have developed an artificial flea-feeding method with blood infected successively with five different Bartonella species. The results demonstrated the ability of these five Bartonella species to persist in C. felis suggesting an ability of fleas to be a potential vector for several Bartonella species. In addition, we demonstrated a regurgitation of Bartonella DNA in uninfected blood used to feed C. felis thus suggesting a potential horizontal transmission of Bartonella through C. felis saliva. On the contrary, no vertical transmission was detected in these artificial conditions.

  4. INFECTION BY Rickettsia felis IN OPOSSUMS (Didelphis sp.) FROM YUCATAN, MEXICO.

    Science.gov (United States)

    Peniche-Lara, Gaspar; Ruiz-Piña, Hugo A; Reyes-Novelo, Enrique; Dzul-Rosado, Karla; Zavala-Castro, Jorge

    2016-01-01

    Rickettsia felis is an emergent pathogen and the causative agent of a typhus-like rickettsiosis in the Americas. Its transmission cycle involves fleas as biological vectors (mainly Ctenocephalides felis) and multiple domestic and synanthropic mammal hosts. Nonetheless, the role of mammals in the cycle of R. felis is not well understood and many efforts are ongoing in different countries of America to clarify it. The present study describes for the first time in Mexico the infection of two species of opossum (Didelphis virginiana and D. marsupialis) by R. felis. A diagnosis was carried out from blood samples by molecular methods through the gltA and 17 kDa genes and sequence determination. Eighty-seven opossum samples were analyzed and 28 were found to be infected (32.1%) from five out of the six studied localities of Yucatan. These findings enable recognition of the potential epidemiological implications for public health of the presence of infected synanthropic Didelphis in households.

  5. Occurrence of Chlamydophila felis, feline herpesvirus 1 and calcivirus in domestic cats of Iran

    OpenAIRE

    Nadi Maazi; Shahram Jamshidi; Payman Kayhani; Hassan Momtaz

    2016-01-01

    Background and Objectives: Feline herpesvirus-1, feline calicivirus and Chlamydophila felis are the main causes of feline upper respiratory tract disease. This study was conducted to identify of FeHV-1, FCV and C. felis infections in domestic cat population and also to estimate the prevalence of each specific infection in Iran.Materials and Methods: The ocular conjunctiva and oropharyngeal specimens obtained from 80 cats were examined using PCR and reverse transcription PCR.Results: FeHV-1 wa...

  6. Embryonic thymic development in fetuses of domestic cats (Felis domesticus

    Directory of Open Access Journals (Sweden)

    Fernanda Rodrigues Agreste

    2012-09-01

    Full Text Available During fetal life, and during the neonatal period, the thymus is a very important immune organ, and is the largest lymphatic organ, which exhibits high lymphopoietic activity as a precursor of lymphopoiesis. Morphological studies on the development of the thymus are rare and only include general information. Given the above, this study aimed to characterize the morphological development of the thymus of embryos and fetuses of domestic cats (Felis domesticus, from natural pregnancy, using macroscopic dissection techniques and light microscopy. The thymus of the cats was pale pink and was resting in the region of the cranial mediastinum, medially to the lungs and dorsally to the base of the heart. Histologically, two distinct regions were observed (cortical and medullar. The medullary region had reticular epithelial cells with large nuclei and dendritic extensions. The fetuses had exponential growth and were more pronounced starting on the 35th day of gestation.

  7. Plasmids and rickettsial evolution: insight from Rickettsia felis.

    Directory of Open Access Journals (Sweden)

    Joseph J Gillespie

    Full Text Available BACKGROUND: The genome sequence of Rickettsia felis revealed a number of rickettsial genetic anomalies that likely contribute not only to a large genome size relative to other rickettsiae, but also to phenotypic oddities that have confounded the categorization of R. felis as either typhus group (TG or spotted fever group (SFG rickettsiae. Most intriguing was the first report from rickettsiae of a conjugative plasmid (pRF that contains 68 putative open reading frames, several of which are predicted to encode proteins with high similarity to conjugative machinery in other plasmid-containing bacteria. METHODOLOGY/PRINCIPAL FINDINGS: Using phylogeny estimation, we determined the mode of inheritance of pRF genes relative to conserved rickettsial chromosomal genes. Phylogenies of chromosomal genes were in agreement with other published rickettsial trees. However, phylogenies including pRF genes yielded different topologies and suggest a close relationship between pRF and ancestral group (AG rickettsiae, including the recently completed genome of R. bellii str. RML369-C. This relatedness is further supported by the distribution of pRF genes across other rickettsiae, as 10 pRF genes (or inactive derivatives also occur in AG (but not SFG rickettsiae, with five of these genes characteristic of typical plasmids. Detailed characterization of pRF genes resulted in two novel findings: the identification of oriV and replication termination regions, and the likelihood that a second proposed plasmid, pRFdelta, is an artifact of the original genome assembly. CONCLUSION/SIGNIFICANCE: Altogether, we propose a new rickettsial classification scheme with the addition of a fourth lineage, transitional group (TRG rickettsiae, that is unique from TG and SFG rickettsiae and harbors genes from possible exchanges with AG rickettsiae via conjugation. We offer insight into the evolution of a plastic plasmid system in rickettsiae, including the role plasmids may have played in

  8. Molecular evidence for the presence of Rickettsia Felis in the feces of wild-living African apes.

    Directory of Open Access Journals (Sweden)

    Alpha Kabinet Keita

    Full Text Available BACKGROUND: Rickettsia felis is a common emerging pathogen detected in mosquitoes in sub-Saharan Africa. We hypothesized that, as with malaria, great apes may be exposed to the infectious bite of infected mosquitoes and release R. felis DNA in their feces. METHODS: We conducted a study of 17 forest sites in Central Africa, testing 1,028 fecal samples from 313 chimpanzees, 430 gorillas and 285 bonobos. The presence of rickettsial DNA was investigated by specific quantitative real-time PCR. Positive results were confirmed by a second PCR using primers and a probe targeting a specific gene for R. felis. All positive samples were sequenced. RESULTS: Overall, 113 samples (11% were positive for the Rickettsia-specific gltA gene, including 25 (22% that were positive for R. felis. The citrate synthase (gltA sequence and outer membrane protein A (ompA sequence analysis indicated 99% identity at the nucleotide level to R. felis. The 88 other samples (78% were negative using R. felis-specific qPCR and were compatible with R. felis-like organisms. CONCLUSION: For the first time, we detected R. felis in wild-living ape feces. This non invasive detection of human pathogens in endangered species opens up new possibilities in the molecular epidemiology and evolutionary analysis of infectious diseases, beside HIV and malaria.

  9. Brainstem acoustic areas in the marine catfish, Arius felis.

    Science.gov (United States)

    McCormick, C A

    2001-03-01

    The marine catfish Arius felis produces low frequency sounds for communication and obstacle detection. It was hypothesized that the utriculus of the inner ear might play an important role in these behaviors. In the current study, brainstem acoustic areas were studied to reveal possible neuroanatomical specializations in utricular processing areas. The first-order octaval nuclei in Arius were identical in number, anatomical characteristics, and organization of saccular, lagenar, and utricular inputs to previous reports of these features in Ictalurus, a closely related species of catfish that does not exhibit the specialized acoustic behaviors present in Arius. Similarly, injections of neural tracer in the acoustic midbrain (nucleus centralis) of Arius revealed afferent and retrograde pathways almost identical to those previously reported in Ictalurus. It is suggested that areas within the primary and higher-order octaval nuclei that utilize utricular input in acoustic processing are likely identical in Arius and Ictalurus. Two sets of higher-order connections in Arius differ from those in Ictalurus. First, Arius apparently lacks the direct input from the anterior octaval nucleus to nucleus centralis reported in Ictalurus. Second, in Arius nucleus centralis projects bilaterally to a strip of neurons positioned ventral to the ventral boundary of the torus semicircularis. This projection is apparently absent in Ictalurus and in the related species Carassius (goldfish), but has been previously reported in Porichthyes, a sound-producing species belonging to a different teleost taxon.

  10. Monitoring Inland Water Turbidity: Contribution of SPOT5 Take5 to Health Hazard Monitoring in West Africa (Bagre Lake, Burkina Faso)

    Science.gov (United States)

    Robert, Elodie; Grippa, Manuela; Kergoat, Laurent; Martinez, Jean-Michel; Pinet, Sylvain; Somdecoste, Tom; Gal, Laetitia

    2016-08-01

    Monitoring turbidity and Surface Suspended Sediment Concentration (SSSC) of inland waters in tropics is essential to assess human health risks, in particular the diarrheal disease risk. In this study, we explore the use of Spot5 time series acquired for the Spot5Take5 Program to monitor turbidity and SSSC on the Bagre Lake (Burkina Faso). Field measurements (turbidity, SSSC, radiometry) are used to evaluate different radiometric indices. The combination of the NIR with a visible band (R or G) is found to be the best suited to retrieve SSSC and turbidity from Spot5 images. Large differences between upstream and downstream areas of the lake are well capture by the Spot5 time series, with large difference in the seasonal maximum both for absolute values and timing. A large sediment transport is observed from upstream to downstream between June and September caused by surface runoff and erosion. The high turbidity values observed suggest that the associated health hazard is potentially high, especially at the beginning of the rainy season and in the upstream areas of the lake.

  11. Experimental infection of cats with Afipia felis and various Bartonella species or subspecies.

    Science.gov (United States)

    Chomel, Bruno B; Kasten, Rickie W; Stuckey, Matthew J; Breitschwerdt, Edward B; Maggi, Ricardo G; Henn, Jennifer B; Koehler, Jane E; Chang, Chao-chin

    2014-08-27

    Based upon prior studies, domestic cats have been shown to be the natural reservoir for Bartonella henselae, Bartonella clarridgeiae and Bartonella koehlerae. However, other Bartonella species, such as Bartonella vinsonii subsp. berkhoffii, Bartonella quintana or Bartonella bovis (ex weissii) have been either isolated from or Bartonella DNA sequences PCR amplified and sequenced. In the late 1980s, before B. henselae was confirmed as the etiological agent of cat scratch disease, Afipia felis had been proposed as the causative agent. In order to determine the feline susceptibility to A. felis, B. vinsonii subsp. berkhoffii, Bartonella rochalimae, B. quintana or B. bovis, we sought to detect the presence of bacteremia and seroconversion in experimentally-inoculated cats. Most of the cats seroconverted, but only the cats inoculated with B. rochalimae became bacteremic, indicating that cats are not natural hosts of A. felis or the other Bartonella species or subspecies tested in this study.

  12. Studying Cat (Felis catus Diabetes: Beware of the Acromegalic Imposter.

    Directory of Open Access Journals (Sweden)

    Stijn J M Niessen

    Full Text Available Naturally occurring diabetes mellitus (DM is common in domestic cats (Felis catus. It has been proposed as a model for human Type 2 DM given many shared features. Small case studies demonstrate feline DM also occurs as a result of insulin resistance due to a somatotrophinoma. The current study estimates the prevalence of hypersomatotropism or acromegaly in the largest cohort of diabetic cats to date, evaluates clinical presentation and ease of recognition. Diabetic cats were screened for hypersomatotropism using serum total insulin-like growth factor-1 (IGF-1; radioimmunoassay, followed by further evaluation of a subset of cases with suggestive IGF-1 (>1000 ng/ml through pituitary imaging and/ or histopathology. Clinicians indicated pre-test suspicion for hypersomatotropism. In total 1221 diabetic cats were screened; 319 (26.1% demonstrated a serum IGF-1>1000 ng/ml (95% confidence interval: 23.6-28.6%. Of these cats a subset of 63 (20% underwent pituitary imaging and 56/63 (89% had a pituitary tumour on computed tomography; an additional three on magnetic resonance imaging and one on necropsy. These data suggest a positive predictive value of serum IGF-1 for hypersomatotropism of 95% (95% confidence interval: 90-100%, thus suggesting the overall hypersomatotropism prevalence among UK diabetic cats to be 24.8% (95% confidence interval: 21.2-28.6%. Only 24% of clinicians indicated a strong pre-test suspicion; most hypersomatotropism cats did not display typical phenotypical acromegaly signs. The current data suggest hypersomatotropism screening should be considered when studying diabetic cats and opportunities exist for comparative acromegaly research, especially in light of the many detected communalities with the human disease.

  13. Amyloidosis in black-footed cats (Felis nigripes).

    Science.gov (United States)

    Terio, K A; O'Brien, T; Lamberski, N; Famula, T R; Munson, L

    2008-05-01

    A high prevalence of systemic amyloidosis was documented in the black-footed cat (Felis nigripes) based on a retrospective review of necropsy tissues (n = 38) submitted as part of ongoing disease surveillance. Some degree of amyloid deposition was present in 33 of 38 (87%) of the examined cats, and amyloidosis was the most common cause of death (26/38, 68%). Amyloid deposition was most severe in the renal medullary interstitium (30/33, 91%) and glomeruli (21/33, 63%). Other common sites included the splenic follicular germinal centers (26/31, 84%), gastric lamina propria (9/23, 39%), and intestinal lamina propria (3/23, 13%). Amyloid in all sites stained with Congo red, and in 13 of 15 (87%) cats, deposits had strong immunoreactivity for canine AA protein by immunohistochemistry. There was no association with concurrent chronic inflammatory conditions (P = .51), suggesting that amyloidosis was not secondary to inflammation. Adrenal cortical hyperplasia, a morphologic indicator of stress that can predispose to amyloid deposition, was similarly not associated (P = .09) with amyloidosis. However, adrenals were not available from the majority of cats without amyloidosis; therefore, further analysis of this risk factor is warranted. Heritability estimation suggested that amyloidosis might be familial in this species. Additionally, tissues from a single free-ranging black-footed cat had small amounts of amyloid deposition, suggesting that there could be a predilection for amyloidosis in this species. Research to identify the protein sequence of serum amyloid A (SAA) in the black-footed cat is needed to further investigate the possibility of an amyloidogenic SAA in this species.

  14. Rickettsial Infections among Ctenocephalides felis and Host Animals during a Flea-Borne Rickettsioses Outbreak in Orange County, California

    Science.gov (United States)

    Fogarty, Carrie; Krueger, Laura; Macaluso, Kevin R.; Odhiambo, Antony; Nguyen, Kiet; Farris, Christina M.; Luce-Fedrow, Alison; Bennett, Stephen; Jiang, Ju; Sun, Sokanary; Cummings, Robert F.; Richards, Allen L.

    2016-01-01

    Due to a resurgence of flea-borne rickettsioses in Orange County, California, we investigated the etiologies of rickettsial infections of Ctenocephalides felis, the predominant fleas species obtained from opossums (Didelphis virginiana) and domestic cats (Felis catus), collected from case exposure sites and other areas in Orange County. In addition, we assessed the prevalence of IgG antibodies against spotted fever group (SFGR) and typhus group (TGR) rickettsiae in opossum sera. Of the 597 flea specimens collected from opossums and cats, 37.2% tested positive for Rickettsia. PCR and sequencing of rickettsial genes obtained from C. felis flea DNA preparations revealed the presence of R. typhi (1.3%), R. felis (28.0%) and R. felis-like organisms (7.5%). Sera from opossums contained TGR-specific (40.84%), but not SFGR-specific antibodies. The detection of R. felis and R. typhi in the C. felis fleas in Orange County highlights the potential risk for human infection with either of these pathogens, and underscores the need for further investigations incorporating specimens from humans, animal hosts, and invertebrate vectors in endemic areas. Such studies will be essential for establishing a link in the ongoing flea-borne rickettsioses outbreaks. PMID:27537367

  15. INFECTION BY Rickettsia felis IN OPOSSUMS (Didelphis sp. FROM YUCATAN, MEXICO

    Directory of Open Access Journals (Sweden)

    Gaspar PENICHE-LARA

    2016-01-01

    Full Text Available Rickettsia felis is an emergent pathogen and the causative agent of a typhus-like rickettsiosis in the Americas. Its transmission cycle involves fleas as biological vectors (mainly Ctenocephalides felis and multiple domestic and synanthropic mammal hosts. Nonetheless, the role of mammals in the cycle of R. felis is not well understood and many efforts are ongoing in different countries of America to clarify it. The present study describes for the first time in Mexico the infection of two species of opossum (Didelphis virginiana and D. marsupialis by R. felis. A diagnosis was carried out from blood samples by molecular methods through the gltAand 17 kDa genes and sequence determination. Eighty-seven opossum samples were analyzed and 28 were found to be infected (32.1% from five out of the six studied localities of Yucatan. These findings enable recognition of the potential epidemiological implications for public health of the presence of infected synanthropic Didelphis in households.

  16. Aspergillus felis sp. nov., an emerging agent of invasive aspergillosis in humans, cats, and dogs.

    Directory of Open Access Journals (Sweden)

    Vanessa R Barrs

    Full Text Available We describe a novel heterothallic species in Aspergillus section Fumigati, namely A. felis (neosartorya-morph isolated from three host species with invasive aspergillosis including a human patient with chronic invasive pulmonary aspergillosis, domestic cats with invasive fungal rhinosinusitis and a dog with disseminated invasive aspergillosis. Disease in all host species was often refractory to aggressive antifungal therapeutic regimens. Four other human isolates previously reported as A. viridinutans were identified as A. felis on comparative sequence analysis of the partial β-tubulin and/or calmodulin genes. A. felis is a heterothallic mold with a fully functioning reproductive cycle, as confirmed by mating-type analysis, induction of teleomorphs within 7 to 10 days in vitro and ascospore germination. Phenotypic analyses show that A. felis can be distinguished from the related species A. viridinutans by its ability to grow at 45°C and from A. fumigatus by its inability to grow at 50°C. Itraconazole and voriconazole cross-resistance was common in vitro.

  17. Possible Role of Rickettsia felis in Acute Febrile Illness among Children in Gabon.

    Science.gov (United States)

    Mourembou, Gaël; Lekana-Douki, Jean Bernard; Mediannikov, Oleg; Nzondo, Sydney Maghendji; Kouna, Lady Charlene; Essone, Jean Claude Biteghe Bi; Fenollar, Florence; Raoult, Didier

    2015-10-01

    Rickettsia felis has been reported to be a cause of fever in sub-Saharan Africa, but this association has been poorly evaluated in Gabon. We assessed the prevalence of this bacterium among children Gabon; the locations were in urban, semiurban, and rural areas. DNA samples from 410 febrile children and 60 afebrile children were analyzed by quantitative PCR. Overall, the prevalence of R. felis among febrile and afebrile children was 10.2% (42/410 children) and 3.3% (2/60 children), respectively. Prevalence differed among febrile children living in areas that are urban (Franceville, 1.3% [1/77]), semiurban (Koulamoutou, 2.1% [3/141]), and rural (Lastourville, 11.2% [15/134]; Fougamou, 39.7% [23/58]). Furthermore, in a rural area (Fougamou), R. felis was significantly more prevalent in febrile (39.7% [23/58]) than afebrile children (5.0% [1/20]). Additional studies are needed to better understand the pathogenic role of R. felis in this part of the world.

  18. Molecular detection of Rickettsia felis and Candidatus Rickettsia asemboensis in fleas from human habitats, Asembo, Kenya.

    Science.gov (United States)

    Jiang, Ju; Maina, Alice N; Knobel, Darryn L; Cleaveland, Sarah; Laudisoit, Anne; Wamburu, Kabura; Ogola, Eric; Parola, Philippe; Breiman, Robert F; Njenga, M Kariuki; Richards, Allen L

    2013-08-01

    The flea-borne rickettsioses murine typhus (Rickettsia typhi) and flea-borne spotted fever (FBSF) (Rickettsia felis) are febrile diseases distributed among humans worldwide. Murine typhus has been known to be endemic to Kenya since the 1950s, but FBSF was only recently documented in northeastern (2010) and western (2012) Kenya. To characterize the potential exposure of humans in Kenya to flea-borne rickettsioses, a total of 330 fleas (134 pools) including 5 species (Xenopsylla cheopis, Ctenocephalides felis, Ctenocephalides canis, Pulex irritans, and Echidnophaga gallinacea) were collected from domestic and peridomestic animals and from human dwellings within Asembo, western Kenya. DNA was extracted from the 134 pooled flea samples and 89 (66.4%) pools tested positively for rickettsial DNA by 2 genus-specific quantitative real-time PCR (qPCR) assays based upon the citrate synthase (gltA) and 17-kD antigen genes and the Rfelis qPCR assay. Sequences from the 17-kD antigen gene, the outer membrane protein (omp)B, and 2 R. felis plasmid genes (pRF and pRFd) of 12 selected rickettsia-positive samples revealed a unique Rickettsia sp. (n=11) and R. felis (n=1). Depiction of the new rickettsia by multilocus sequence typing (MLST) targeting the 16S rRNA (rrs), 17-kD antigen gene, gltA, ompA, ompB, and surface cell antigen 4 (sca4), shows that it is most closely related to R. felis but genetically dissimilar enough to be considered a separate species provisionally named Candidatus Rickettsia asemboensis. Subsequently, 81 of the 134 (60.4%) flea pools tested positively for Candidatus Rickettsia asemboensis by a newly developed agent-specific qPCR assay, Rasemb. R. felis was identified in 9 of the 134 (6.7%) flea pools, and R. typhi the causative agent of murine typhus was not detected in any of 78 rickettsia-positive pools assessed using a species-specific qPCR assay, Rtyph. Two pools were found to contain both R. felis and Candidatus Rickettsia asemboensis DNA and 1 pool

  19. A new variant of endemic pemphigus foliaceus in El-Bagre, Colombia: the Hardy-Weinberg-Castle law and linked short tandem repeats

    Directory of Open Access Journals (Sweden)

    Ana María Abreu Velez

    2009-01-01

    Full Text Available Background : We reported a new variant of endemic pemphigus foliaceus in El Bagre, Colombia. Aims : Our study performed Complex Segregation Analysis (CSA and short tandem repeats to discriminate between environmental and/or genetic factors in this disorder. Materials and Methods: The CSA analysis was carried out according to the unified model, implemented using the transmission probabilities implemented in the computer program POINTER, and evaluated by using a software package for population genetic data analysis (GDA, Arlequin. We performed pedigree analyses by using Cyrillic 2.1 software, with a total of 30 families with 50 probands (47 males and 3 females tested. In parallel to the CSA, we tested for the presence of short tandem repeats from HLA class II, DQ alpha 1, involving the gene locus D6S291 by using the Hardy-Weinberg- Castle law. Results : Our results indicate that the best model of inheritance in this disease is a mixed model, with multifactorial effects within a recessive genotype. Two types of possible segregation patterns were found; one with strong recessive penetrance in families whose phenotype is more Amerindian-like, and another of possible somatic mutations. Conclusion : The penetrance of 10% or less in female patients 60 years of age or older indicates that hormones could protect younger females. The greatest risk factor for men being affected by the disorder was the NN genotype. These findings are only possible due to somatic mutations, and/or strong environmental effects. We also found a protective role for two genetic loci (D6S1019 AND D6S439 in the control group.

  20. Environmental changes and microbiological health risks. Satellite-derived turbidity: an indicator of "health hazard" for surface water in West Africa (Bagre lake, Burkina Faso).

    Science.gov (United States)

    Robert, E.; Grippa, M.; Kergoat, L.; Martinez, J.; Pinet, S.; Gal, L.; Soumaguel, N.

    2015-12-01

    A significant correlation exists between the concentration of parasites, bacteria and some water quality parameters including surface suspended solids (SSS) and turbidity. Suspended particles can carry viruses and pathogenic bacteria affecting human health and foster their development. High SSS, associated with high turbidity, can therefore be considered as a vector of microbiological contaminants, causing diarrheal diseases. Few studies have focused on the turbidity parameter in rural Africa, while many cases of intestinal parasitic infections are due to the consumption of unsafe water from ponds, lakes, and rivers. Monitoring turbidity may therefore contribute to health hazard monitoring. Turbidity refers to the optical properties of water and is known to impact water reflectance in the visible and near-infrared domain. Ideally, its spatial and temporal variability requires the use of high temporal resolution (MODIS) and spatial resolution (Landsat, SPOT, Sentinel-2). Here we investigate turbidity in West-Africa. Various algorithms and indices proposed in the literature for inland waters are applied to MODIS series and to Landsat 7 and 8 CDR images, and SPOT5 images. The data and algorithms are evaluated with field measurements: turbidity, SSS, and hyperspectral ground radiometry. We show that turbidity of the Bagre Lake displays a strong increase over 2000-2015, associated with the corresponding increase of the red and NIR reflectances, as well as a reduction of the seasonal variations. Water level derived from the Jason 2 altimeter does not explain such variations. The most probable hypothesis is a change in land use (increase in bare and degraded soils), that leads to an increase in the particles transported by surface runoff to the lake. Such an increase in turbidity reinforces the health risk. We will discuss the link between turbidity and health in view of data from health centers on diarrheal diseases as well as data on practices and uses of populations.

  1. Conservation inequality and the charismatic cat: Felis felicis

    Directory of Open Access Journals (Sweden)

    E.A. Macdonald

    2015-01-01

    . While the felids are widely regarded as a popular taxonomic group, the great extent to which they appealed to our respondents emphasises their potential as ambassadors for conservation. Indeed, the big cats were so highly rated that we might think of them as one, Felis felicis: a globally powerful flagship for conservation.

  2. Prevalence of feline herpesvirus-1, feline calicivirus, Chlamydophila felis and Mycoplasma felis DNA and associated risk factors in cats in Spain with upper respiratory tract disease, conjunctivitis and/or gingivostomatitis.

    Science.gov (United States)

    Fernandez, Mireia; Manzanilla, Edgar G; Lloret, Albert; León, Marta; Thibault, Jean-Christophe

    2017-04-01

    Objectives Our objective was to perform the first multicentric study in Spain to evaluate the prevalence of feline herpesvirus-1 (FHV-1), feline calicivirus (FCV), Chlamydophila felis and Mycoplasma felis in cats with upper respiratory tract disease (URTD), conjunctivitis and/or gingivostomatitis (GS) compared with control cats; and to evaluate risk factors for these clinical conditions. Methods Conjunctival and oropharyngeal swabs were collected and a questionnaire regarding signalment, lifestyle, vaccination history and clinical signs was obtained for each cat. Swabs were tested for each pathogen by real-time PCR. Results The study population consisted of 358 cats, including 98 control cats. Among the 260 diseased cats, 127 cats presented with URTD, 149 cats had conjunctivitis, 154 cats were suffering GS; many cats presented more than one clinical condition. The prevalence observed of FHV-1, FCV, C felis and M felis was, respectively, 28.3%, 48.0%, 20.5% and 46.5% in cats with URTD; 24.2%, 43.6%, 19.5% and 38.3% in cats with conjunctivitis; and 15.6%, 58.4%, 9.1% and 37.7% in cats with GS. Prevalences in the control group were 6.1%, 15.3%, 2.0% and 20.4%, respectively. Coinfections were common among all groups of cats. Risk factors were identified for all groups. FHV-1, FCV and C felis were associated with URTD and conjunctivitis. FCV was strongly associated with GS. M felis was present in a high percentage of the population in all groups, but its role in these clinical conditions remains uncertain. Vaccination was protective for URTD and GS but not for conjunctivitis. Conclusions and relevance This epidemiological study describes, for the first time, prevalence for FHV-1, FCV, C felis and M felis in Spain. In general, the prevalences found are similar to those reported in other countries. Factors associated with disease expression were also identified, which are relevant for practitioners.

  3. Clinical and laboratorial evidence of Rickettsia felis infections in Latin America Evidência clínica e laboratorial de infecções por Rickettsia felis na América Latina

    Directory of Open Access Journals (Sweden)

    Márcio Antônio Moreira Galvão

    2004-06-01

    Full Text Available After the discovery and initial characterization of Rickettsia felis in 1992 by Azad and cols, and the subsequent first description of a human case of infection in 1994, there have been two communications of human rickettsiosis cases caused by Rickettsia felis in Latin America. The first one was published in 2000 by Zavala-Velazquez and cols in Mexico. In 2001 Raoult and cols described the occurrence of two human cases of Rickettsia felis rickettsiosis in Brazil. In the present discussion these two articles were compared and after the description of the principal signs and symptoms, it was concluded that more studies are needed with descriptions of a greater number of patients to establish the true frequency of the clinical signs and symptoms present in Rickettsia felis rickettsiosis.Depois da descoberta e caracterização inicial da Rickettsia felis em 1992 por Azad e cols, e à descrição subseqüente do primeiro caso de infecção humana em 1994, houveram duas comunicações de rickettsioses causadas por Rickettsia felis na América Latina. A primeira foi feita por Zavala-Velazquez e cols em 2000 no México. Em 2001, Raoult e cols descreveram a ocorrência de dois casos humanos de rickettsiose por Rickettsia felis no Brasil. Na presente discussão, esses dois artigos foram comparados, e depois da descrição dos principais sinais e sintomas, conclui-se que outros estudos são necessários, com a participação de um maior número de pacientes, para se estabelecer a verdadeira freqüência dos sinais clínicos e sintomas presentes nas rickettsioses por Rickettsia felis.

  4. Blood meal identification in off-host cat fleas (Ctenocephalides felis) from a plague-endemic region of Uganda.

    Science.gov (United States)

    Graham, Christine B; Borchert, Jeff N; Black, William C; Atiku, Linda A; Mpanga, Joseph T; Boegler, Karen A; Moore, Sean M; Gage, Kenneth L; Eisen, Rebecca J

    2013-02-01

    The cat flea, Ctenocephalides felis, is an inefficient vector of the plague bacterium (Yersinia pestis) and is the predominant off-host flea species in human habitations in the West Nile region, an established plague focus in northwest Uganda. To determine if C. felis might serve as a Y. pestis bridging vector in the West Nile region, we collected on- and off-host fleas from human habitations and used a real-time polymerase chain reaction-based assay to estimate the proportion of off-host C. felis that had fed on humans and the proportion that had fed on potentially infectious rodents or shrews. Our findings indicate that cat fleas in human habitations in the West Nile region feed primarily on domesticated species. We conclude that C. felis is unlikely to serve as a Y. pestis bridging vector in this region.

  5. Desenvolvimento dos fungos Metarhizium anisopliae (Metschnikoff, 1879 Sorokin, 1883 E Beauveria bassiana (Balsamo Vuillemin, 1912 sobre Ctenocephalides felis felis (Bouché, 1835 Development of the fungi Metarhizium anisopliae (Metschnikoff, 1879 Sorokin, 1883 and Beauveria bassiana (Balsamo Vuillemin, 1912 on the Ctenophephalides felis felis (Bouché, 1835

    Directory of Open Access Journals (Sweden)

    Denise R. De Melo

    2007-09-01

    Full Text Available A pulga Ctenocephalides felis felis é um parasita causador dermatites alérgicas e também pode transmitir diversos agentes etiológicos aos animais domésticos e aos homens. O objetivo deste trabalho foi verificar o desenvolvimento do fungo sobre a cutícula da pulga, através da microscopia eletrônica de varredura. Os isolados fúngicos testados foram o Metarhizium anisopliae 959 e Beauveria bassiana 986, ambos na concentração 10(8 conídios/ml. Após a exposição dos isolados fúngicos no período de duas, 15, 26 e 96 horas , o material foi processado para a microscopia eletrônica de varredura. Com a obtenção das micrografias, pode-se observar que com 2 horas após exposição aos fungos, os conídios estavam aderidos por toda a cutícula, situando-se preferencialmente nas membranas intersegmentais do abdome. Com 15 horas observou-se a formação do tubo de germinação e a cabeça do apressório e após 26 horas foi possível observar as ramificações e o engrossamento das hifas sobre a cutícula das pulgas. Os resultados indicam que os fungos testados foram capazes de se desenvolver sobre a cutícula de C. f. felis.The flea Ctenocephalides felis felis is a parasite that causes allergic dermatitis and also may transmit etiologic agents to domestic animals and humans. This study investigated by scanning electron microscopy the development of entomopathogenic fungi on flea cuticle. Fleas were exposed to conidia (10(8 ml-1 of Metarhizium anisopliae (isolate 959 or Beauveria bassiana (isolate 986. Following standard protocols for electron microscopy, the specimens were prepared 2, 15, 26 and 96 h after infection. The micrography revealed that 2 h after fungus exposure, conidia attachments encompassed the entire flea cuticle, especially on abdominal intersegmental membranes. The emergence of germ tubes and appressoria formation occurred at 15 h, thickening and branching of hyphae on the flea cuticle was noted at 26 h. Therefore, both of

  6. The genetic integrity of the ex situ population of the European wildcat (Felis silvestris silvestris) is seriously threatened by introgression from domestic cats (Felis silvestris catus).

    Science.gov (United States)

    Witzenberger, Kathrin A; Hochkirch, Axel

    2014-01-01

    Studies on the genetic diversity and relatedness of zoo populations are crucial for implementing successful breeding programmes. The European wildcat, Felis s. silvestris, is subject to intensive conservation measures, including captive breeding and reintroduction. We here present the first systematic genetic analysis of the captive population of Felis s. silvestris in comparison with a natural wild population. We used microsatellites and mtDNA sequencing to assess genetic diversity, structure and integrity of the ex situ population. Our results show that the ex situ population of the European wildcat is highly structured and that it has a higher genetic diversity than the studied wild population. Some genetic clusters matched the breeding lines of certain zoos or groups of zoos that often exchanged individuals. Two mitochondrial haplotype groups were detected in the in situ populations, one of which was closely related to the most common haplotype found in domestic cats, suggesting past introgression in the wild. Although native haplotypes were also found in the captive population, the majority (68%) of captive individuals shared a common mtDNA haplotype with the domestic cat (Felis s. catus). Only six captive individuals (7.7%) were assigned as wildcats in the STRUCTURE analysis (at K = 2), two of which had domestic cat mtDNA haplotypes and only two captive individuals were assigned as purebred wildcats by NewHybrids. These results suggest that the high genetic diversity of the captive population has been caused by admixture with domestic cats. Therefore, the captive population cannot be recommended for further breeding and reintroduction.

  7. The genetic integrity of the ex situ population of the European wildcat (Felis silvestris silvestris is seriously threatened by introgression from domestic cats (Felis silvestris catus.

    Directory of Open Access Journals (Sweden)

    Kathrin A Witzenberger

    Full Text Available Studies on the genetic diversity and relatedness of zoo populations are crucial for implementing successful breeding programmes. The European wildcat, Felis s. silvestris, is subject to intensive conservation measures, including captive breeding and reintroduction. We here present the first systematic genetic analysis of the captive population of Felis s. silvestris in comparison with a natural wild population. We used microsatellites and mtDNA sequencing to assess genetic diversity, structure and integrity of the ex situ population. Our results show that the ex situ population of the European wildcat is highly structured and that it has a higher genetic diversity than the studied wild population. Some genetic clusters matched the breeding lines of certain zoos or groups of zoos that often exchanged individuals. Two mitochondrial haplotype groups were detected in the in situ populations, one of which was closely related to the most common haplotype found in domestic cats, suggesting past introgression in the wild. Although native haplotypes were also found in the captive population, the majority (68% of captive individuals shared a common mtDNA haplotype with the domestic cat (Felis s. catus. Only six captive individuals (7.7% were assigned as wildcats in the STRUCTURE analysis (at K = 2, two of which had domestic cat mtDNA haplotypes and only two captive individuals were assigned as purebred wildcats by NewHybrids. These results suggest that the high genetic diversity of the captive population has been caused by admixture with domestic cats. Therefore, the captive population cannot be recommended for further breeding and reintroduction.

  8. Rickettsia typhi and Rickettsia felis in Xenopsylla cheopis and Leptopsylla segnis parasitizing rats in Cyprus.

    Science.gov (United States)

    Christou, Christos; Psaroulaki, Anna; Antoniou, Maria; Toumazos, Pavlos; Ioannou, Ioannis; Mazeris, Apostolos; Chochlakis, Dimosthenis; Tselentis, Yannis

    2010-12-01

    Fleas collected from rats during a three-year period (2000-2003) in 51 areas of all provinces of Cyprus were tested by molecular analysis to characterize the prevalence and identity of fleaborne rickettsiae. Rickettsia typhi, the causative agent of murine typhus, was detected in Xenopsylla cheopis (4%) and in Leptopsylla segnis (6.6%). Rickettsia felis was detected in X. cheopis (1%). This is the first report of R. typhi in X. cheopis and L. segnis from rats, in Cyprus, and the first report of R. felis in X. cheopis in Europe. The role of fleas (mainly X. cheopis) was confirmed in the epidemiologic cycle of murine typhus in Cyprus by interrelation of current results with those of previous studies. The geographic distribution of fleas coincided with the geographic distribution of the pathogen they can harbor, which emphasizes the potential risk of flea-transmitted infections in Cyprus.

  9. Progress in research of Rickettsia felis%猫立克次体研究进展

    Institute of Scientific and Technical Information of China (English)

    刘杨; 杨雨; 陈萍; 赵锋; 钟玮

    2015-01-01

    猫立克次体(Rickettsia felis)可以引起蚤传斑点热(Flea-Borne Spotted Fever,FBSF),主要传播媒介是猫蚤(Ctenocephalides felis).自上世纪90年代首次发现以来,已被证实分布于地球上除南极以外的各大洲.本文对猫立克次体的传播媒介、检测方法以及基因组学研究进展进行了综述,并讨论了在全球气候变化的背景下,开展猫立克次体研究的重要意义.

  10. Detection of Rickettsia felis, Rickettsia typhi, Bartonella Species and Yersinia pestis in Fleas (Siphonaptera from Africa.

    Directory of Open Access Journals (Sweden)

    Hamza Leulmi

    2014-10-01

    Full Text Available Little is known about the presence/absence and prevalence of Rickettsia spp, Bartonella spp. and Yersinia pestis in domestic and urban flea populations in tropical and subtropical African countries.Fleas collected in Benin, the United Republic of Tanzania and the Democratic Republic of the Congo were investigated for the presence and identity of Rickettsia spp., Bartonella spp. and Yersinia pestis using two qPCR systems or qPCR and standard PCR. In Xenopsylla cheopis fleas collected from Cotonou (Benin, Rickettsia typhi was detected in 1% (2/199, and an uncultured Bartonella sp. was detected in 34.7% (69/199. In the Lushoto district (United Republic of Tanzania, R. typhi DNA was detected in 10% (2/20 of Xenopsylla brasiliensis, and Rickettsia felis was detected in 65% (13/20 of Ctenocephalides felis strongylus, 71.4% (5/7 of Ctenocephalides canis and 25% (5/20 of Ctenophthalmus calceatus calceatus. In the Democratic Republic of the Congo, R. felis was detected in 56.5% (13/23 of Ct. f. felis from Kinshasa, in 26.3% (10/38 of Ct. f. felis and 9% (1/11 of Leptopsylla aethiopica aethiopica from Ituri district and in 19.2% (5/26 of Ct. f. strongylus and 4.7% (1/21 of Echidnophaga gallinacea. Bartonella sp. was also detected in 36.3% (4/11 of L. a. aethiopica. Finally, in Ituri, Y. pestis DNA was detected in 3.8% (1/26 of Ct. f. strongylus and 10% (3/30 of Pulex irritans from the villages of Wanyale and Zaa.Most flea-borne infections are neglected diseases which should be monitored systematically in domestic rural and urban human populations to assess their epidemiological and clinical relevance. Finally, the presence of Y. pestis DNA in fleas captured in households was unexpected and raises a series of questions regarding the role of free fleas in the transmission of plague in rural Africa, especially in remote areas where the flea density in houses is high.

  11. Hexane Extracts of Calophyllum brasiliense Inhibit the Development of Gastric Preneoplasia in Helicobacter felis Infected INS-Gas Mice

    Science.gov (United States)

    Lemos, Larissa M. S.; Miyajima, Fabio; Castilho, Geovane R. C.; Martins, Domingos Tabajara O.; Pritchard, D. Mark; Burkitt, Michael D.

    2017-01-01

    Objectives: Indigenous Latin American populations have used extracts from Calophyllum brasiliense, a native hardwood, to treat gastrointestinal symptoms for generations. The hexane extract of Calophyllum brasiliense stem bark (HECb) protects against ethanol-mediated gastric ulceration in Swiss–Webster mice. We investigated whether HECb inhibits the development of gastric epithelial pathology following Helicobacter felis infection of INS-Gas mice. Materials and Methods: Groups of five male, 6-week-old INS-Gas mice were colonized with H. felis by gavage. From 2 weeks after colonization their drinking water was supplemented with 2% Tween20 (vehicle), low dose HECb (33 mg/L, lHECb) or high dose HECb (133 mg/L, hHECb). Equivalent uninfected groups were studied. Animals were culled 6 weeks after H. felis colonization. Preneoplastic pathology was quantified using established histological criteria. Gastric epithelial cell turnover was quantified by immunohistochemistry for Ki67 and active-caspase 3. Cytokines were quantified using an electrochemiluminescence assay. Results: Vehicle-treated H. felis infected mice exhibited higher gastric atrophy scores than similarly treated uninfected mice (mean atrophy score 5.6 ± 0.87 SEM vs. 2.2 ± 0.58, p < 0.01). The same pattern was observed following lHECb. Following hHECb treatment, H. felis status did not significantly alter atrophy scores. Gastric epithelial apoptosis was not altered by H. felis or HECb administration. Amongst vehicle-treated mice, gastric epithelial cell proliferation was increased 2.8-fold in infected compared to uninfected animals (p < 0.01). Administration of either lHECb or hHECb reduced proliferation in infected mice to levels similar to uninfected mice. A Th17 polarized response to H. felis infection was observed in all infected groups. hHECb attenuated IFN-γ, IL-6, and TNF production following H. felis infection [70% (p < 0.01), 67% (p < 0.01), and 41% (p < 0.05) reduction vs. vehicle, respectively

  12. Mercury in fishes of the Chassahowitzka National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From August 24 to 28, 1990, spotted seatrout (Cynoscion nebulosus), hardhead catfish (Arius felis) and gafftopsail catfish (Bagre marinus) were collected from...

  13. Echinococcus multilocularis detection in the intestines and feces of free-ranging domestic cats (Felis s. catus) and European wildcats (Felis s. silvestris) from northeastern France.

    Science.gov (United States)

    Umhang, Gérald; Forin-Wiart, Marie-Amélie; Hormaz, Vanessa; Caillot, Christophe; Boucher, Jean-Marc; Poulle, Marie-Lazarine; Franck, Boué

    2015-11-30

    Experimental studies have demonstrated that cats can be infected by Echinococcus multilocularis, although few data are available concerning their natural infection. This study was designed to compare experimental findings with information on the prevalence of natural E. multilocularis infections of cats in a rural high endemic area. Of 19 intestines of domestic cats (Felis s. catus) and five of European wildcats (Felis s. silvestris) analyzed by segmental sedimentation and counting technique (SSCT), infection by E. multilocularis was observed for one individual of each species, resulting in a prevalence estimated at 5%, (CI95%: 1-26) in domestic cats and at 20% (CI95%: 1-72) in wildcats. High worm burdens (680 and 7040) were noted, but comprised only immature worms. The same EmsB microsatellite profile obtained from the worms' DNA was observed in the two cats as in foxes from the same area and from other European countries. The presence of E. multilocularis DNA was diagnosed in 3.1% (10/321) of the domestic cat feces collected on the field in two villages. However, no E. multilocularis eggs were found after flotation with zinc chloride of the positive feces. The detection of DNA from E. multilocularis was thought to be due to the presence of cells from worms untied from the intestine and corresponding to prepatent infection or due to the digested metacestode. These results from E. multilocularis presence in wild and domestic cat populations agree with those previously obtained by experimental infections. These findings support that these cats play an insignificant role in E. multilocularis transmission, even in a "highly endemic" region. Nevertheless, since the presence of thick-shelled E. multilocularis eggs from cats has already been reported, the associated zoonotic risk cannot be totally ruled out, even if it is very low.

  14. Design of FELiChEM, the first infrared free-electron laser user facility in China

    CERN Document Server

    Li, He-Ting; Zhang, Shan-Cai; Wang, Lin; Yang, Yong-Liang

    2016-01-01

    FELiChEM is a new experimental facility under construction at University of Science and Technology of China (USTC), whose core device is two free electron laser oscillators generating middle-infrared and far-infrared laser and covering the spectral range of 2.5-200 ?m. It will be a dedicated infrared light source aiming at energy chemistry research. We present the brief design of FEL oscillators with the emphasis put on the middle-infrared oscillator. Most of the basic parameters are determined and the anticipated performance of the output radiation is given. The first light of FELiChEM is targeted for the end of 2017.

  15. Hallazgo de un hemopárasito eritrocítico tipo Cytauxzoon en Felis catus domesticus

    Directory of Open Access Journals (Sweden)

    Baraboglia, E. R.

    2011-07-01

    Full Text Available ResumenSe comunica el hallazgo de protozoarios eritrocíticos en Felis catusdomesticus con características morfológicas de Cytauxzoon, no pudiendoarriesgarse la especie por los detalles que exponemos, como tampoco señalar cual serían el o los vectores que actúan en nuestros casos por las particularidades que surgen de los mismos.SummaryWe show the finding of erythrocytic parasites in Felis catus domesticus similar to Cytauxzoon detected by us. It is not possible to confirm the species not either the vector.

  16. PCR amplification of a multi-copy mitochondrial gene (cox3) improves detection of Cytauxzoon felis infection as compared to a ribosomal gene (18S).

    Science.gov (United States)

    Schreeg, Megan E; Marr, Henry S; Griffith, Emily H; Tarigo, Jaime L; Bird, David M; Reichard, Mason V; Cohn, Leah A; Levy, Michael G; Birkenheuer, Adam J

    2016-07-30

    Cytauxzoon felis is a tick-transmitted protozoan parasite that infects felids. Clinical disease caused by acute C. felis infection rapidly progresses in domestic cats, leading to high morbidity and mortality. Accurately diagnosing cytauxzoonosis as soon as possible during acute infection would allow for earlier initiation of antiprotozoal therapy which could lead to higher survival rates. Molecular detection of parasite rRNA genes (18S) by PCR has previously been shown to be a sensitive method of diagnosing C. felis infections. Based on evidence from related apicomplexan species, we hypothesized that C. felis mitochondrial genes would exist at higher copy numbers than 18S and would be a more sensitive diagnostic target. In this study we have designed a PCR assay targeting the C. felis mitochondrial gene cytochrome c oxidase subunit III (cox3). Herein we demonstrate that (1) the cox3 PCR can detect as low as 1 copy of DNA target and can detect C. felis in samples with known mitochondrial sequence heterogeneity, (2) cox3 copy number is increased relative to 18S in blood and tissue samples from acutely infected cats, and (3) the cox3 PCR is more sensitive than 18S PCR for detection of C. felis during early infections.

  17. Consistent detection of Felis domesticus papillomavirus 2 DNA sequences within feline viral plaques.

    Science.gov (United States)

    Munday, John S; Peters-Kennedy, Jeanine

    2010-11-01

    Viral plaques are well recognized skin lesions of cats. They are thought to be caused by papillomavirus infection; however, the causative papillomavirus is uncertain. In the current study, polymerase chain reaction using 2 consensus primer sets and 1 primer set specific for Felis domesticus papillomavirus 2 (FdPV-2) was used to amplify DNA from a series of 14 feline viral plaques. The FdPV-2 sequences were detected in all 14 viral plaques by the specific primers but in only 1 of 14 feline cutaneous trichoblastomas. Papillomavirus DNA was amplified from 8 plaques using the consensus primers. Sequences from FdPV-2 were amplified using the consensus primers from 4 plaques. In addition, 3 plaques contained papillomavirus DNA sequences from Felis domesticus papillomavirus sequence MY1, and a previously unreported papillomavirus DNA sequence was amplified from 1 plaque. As FdPV-2 was consistently present within the plaques, this suggests that this papillomavirus is the likely etiologic agent. Feline viral plaques can undergo neoplastic transformation to Bowenoid in situ carcinomas (BISCs). As FdPV-2 DNA is frequently present within BISCs, this suggests that FdPV-2 induces viral plaque formation and then remains detectible after neoplastic transformation.

  18. Efficacy of an imidacloprid 10 % / flumethrin 4.5 % collar (Seresto®, Bayer) for preventing the transmission of Cytauxzoon felis to domestic cats by Amblyomma americanum.

    Science.gov (United States)

    Reichard, Mason V; Thomas, Jennifer E; Arther, Robert G; Hostetler, Joseph A; Raetzel, Kara L; Meinkoth, James H; Little, Susan E

    2013-08-01

    Infection of Cytauxzoon felis in domestic cats produces a severe disease characterised by fever, lethargy, inappetence, anorexia, depression, dehydration, icterus and often death. Transmission of C. felis to cats is dependent on being fed upon by infected Amblyomma americanum (lone star ticks). The purpose of the present study was to determine if application of a 10 % imidacloprid/4.5 % flumethrin collar (Seresto®, Bayer) on cats prevents transmission of C. felis by repelling ticks. Twenty cats were randomised to either a treated (n = 10) or non-treated control group (n = 10) based on their susceptibility to ticks. Cats of high, medium and low tick susceptibility were represented in both groups. Treated cats were fitted with 10 % imidacloprid/4.5 % flumethrin collars on study day 0 and both groups were then infested with C. felis-infected A. americanum on study day 30. Tick thumb counts were performed at 24 and 48 hours post infestation. Transmission of C. felis was determined by examining blood of cats by DNA extraction followed by PCR amplification with piroplasm-specific primers. Ticks did not attach to any of the 10 % imidacloprid/4.5 % flumethrin- treated cats. However, ticks attached and fed on all the non-treated control cats. The geometric mean number of ticks attached to the non-treated control cats at 24 and 48 hours was 15.3 and 14.2, respectively. Cytauxzoon felis was transmitted to 9 of 10 (90 %) non-treated control cats; C. felis was not transmitted to any of the treated cats. Transmission of C. felis to the non-treated cats was first detected between 8 and 16 days post infestation. Our results indicate that application of the 10 % imidacloprid/4.5 % flumethrin collar to cats prevented ticks from attaching, feeding and transmitting C. felis.

  19. CHARACTERIZATION OF THE NUTRITIONAL QUALITY OF THE MEAT IN SOME SPECIES OF CATFISH: A REVIEW REVISIÓN: CARACTERIZACIÓN DE LA CALIDAD NUTRICIONAL DE LA CARNE EN ALGUNAS ESPECIES DE BAGRE

    Directory of Open Access Journals (Sweden)

    Nubia Estella Cruz Casallas

    2012-06-01

    Full Text Available Abstract. One of the most consumed fish in the world is Silurid, also called "leather fish" or catfish, whose main characteristic is the absence of intramuscular bones and scales, as well as its high productivity. In recent years, the nutritional characterization of the meat of some of these species has been carried out, finding that, although the proximal composition is within the broad ranges for fish, the fat content provides a lower proportion of polyunsaturated fatty acids (PUFA particularly as regards omega-3 (w-3, furthermore the w-6/w-3 ratio is within the proscriptions of the World Health Organization (WHO for many of these species of catfish. Likewise, the contents of eicosapentaenoic acid (EPA, docosahexaenoic acid (DHA and amino acids, minerals and vitamins reveal a high variability between individuals and species associated with the type of cultivation and dietary habits and also with the age and weight at slaughter. Furthermore quality parameters have been defined in relation to susceptibility to autolysis, oxidation and hydrolysis of fats and disturbances caused by microorganisms that cause decisive changes in the physicochemical, microbiological and sensory characteristics. This review compiles current information regarding the nutritional composition of catfish meat and the quality parameters.Resumen. Una de las carnes de pescado de mayor consumo en el mundo es la de Silúridos, también denominados peces de cuero o bagres, cuya principal característica es la ausencia de espinas intramusculares y de escamas, además de su alta productividad. En los últimos años se ha logrado realizar la caracterización nutricional de la carne de algunas de estas especies, hallándose que aunque la composición proximal se encuentra dentro de los rangos generales para peces, el contenido de grasa ofrece menor proporción de ácidos grasos poliinsaturados (AGP particularmente en lo referente a la serie omega 3 (w-3, aunque la relación w-6/w-3

  20. Implantação de colônia de Ctenocephalides felis felis (Bouché, 1835 e determinação do período de desenvolvimento dos estágios imaturos sob condições controladas / Implantation of a Ctenocephalides felis felis (Bouché, 1835 colony and determination of the development period of the immature stages under controlled conditions

    Directory of Open Access Journals (Sweden)

    Karina Carrão Castagnolli

    2008-09-01

    Full Text Available O presente estudo teve como objetivo a implantação de uma colônia de pulgas (Ctenocephalides felis felis, para determinação do período de desenvolvimento dos estágios imaturos desse inseto, quando mantido emcondições controladas. Para isto, gatos foram infestados artifi cialmente com estágios adultos de C. felis felis e mantidos em gaiolas metálicas suspensas. Diariamente, durante trinta dias, os ovos de pulgas provenientes dos gatos eram recolhidos e mantidos em estufa do tipo B.O.D., com Tode 28±1oC e umidade relativa de 75%. O tempo de eclosão larval foi em média de dois a quatro dias, as pré-pupas surgiram no período de seis a nove dias, a pupação iniciou-se com nove a onze dias e a emergência dos adultos variou de quatorze a vinte dias. Nessas condições climáticas associadas à dieta adotada, foi possível uma recuperação de adultos de aproximadamente 90%, resultados favoráveis à manutenção de umacolônia.

  1. Seroprevalence of Toxoplasma gondii and Neospora caninum in feral cats (Felis silvestris catus) in Majorca, Balearic Islands, Spain

    Science.gov (United States)

    Felids are important in the epidemiology of Toxoplasma gondii infection because they are the only hosts that can excrete environmentally-resistant oocysts. Antibodies to T. gondii and Neospora caninum were determined in serum samples from 59 feral cats (Felis silvestris catus) captured in baited tra...

  2. Shell-vial culture and real-time PCR applied to Rickettsia typhi and Rickettsia felis detection.

    Science.gov (United States)

    Segura, Ferran; Pons, Immaculada; Pla, Júlia; Nogueras, María-Mercedes

    2015-11-01

    Murine typhus is a zoonosis transmitted by fleas, whose etiological agent is Rickettsia typhi. Rickettsia felis infection can produces similar symptoms. Both are intracellular microorganisms. Therefore, their diagnosis is difficult and their infections can be misdiagnosed. Early diagnosis prevents severity and inappropriate treatment regimens. Serology can't be applied during the early stages of infection because it requires seroconversion. Shell-vial (SV) culture assay is a powerful tool to detect Rickettsia. The aim of the study was to optimize SV using a real-time PCR as monitoring method. Moreover, the study analyzes which antibiotics are useful to isolate these microorganisms from fleas avoiding contamination by other bacteria. For the first purpose, SVs were inoculated with each microorganism. They were incubated at different temperatures and monitored by real-time PCR and classical methods (Gimenez staining and indirect immunofluorescence assay). R. typhi grew at all temperatures. R. felis grew at 28 and 32 °C. Real-time PCR was more sensitive than classical methods and it detected microorganisms much earlier. Besides, the assay sensitivity was improved by increasing the number of SV. For the second purpose, microorganisms and fleas were incubated and monitored in different concentrations of antibiotics. Gentamicin, sufamethoxazole, trimethoprim were useful for R. typhi isolation. Gentamicin, streptomycin, penicillin, and amphotericin B were useful for R. felis isolation. Finally, the optimized conditions were used to isolate R. felis from fleas collected at a veterinary clinic. R. felis was isolated at 28 and 32 °C. However, successful establishment of cultures were not possible probably due to sub-optimal conditions of samples.

  3. Diseases of the European wildcat (Felis silvestris Schreber, 1777) in Great Britain.

    Science.gov (United States)

    McOrist, S

    1992-12-01

    The author describes an examination conducted in collaboration with the Nature Conservancy Council of Great Britain into the status with regard to disease, conservation and genetics of the European wildcat (Felis silvestris). Feline leukaemia virus (FeLV) infection was detected by positive enzyme-linked immunosorbent assay in blood from 2 of 23 wildcats and was tested and confirmed by FeLV isolation in one of the two cats. This is the first time the virus has been clearly demonstrated in a free-living felid, other than the domestic cat. Toxoplasmosis was detected in all cats tested, but neither feline coronavirus nor feline immunodeficiency virus was detected in any sample. The genetic analysis indicated that only 8 of 42 wildcats tested were genetically distinct. These were mainly located in the western highlands of Scotland where "relict" populations may have survived. Interbreeding with domestic cats and persecution by trapping and hunting represent major threats to the survival of the European wildcat.

  4. A hybrid type undulator for far-infrared FELs at FELI

    Energy Technology Data Exchange (ETDEWEB)

    Zako, A.; Miyauchi, Y.; Koga, A. [Free Electron Laser Research Institute, Inc., Osaka (Japan)] [and others

    1995-12-31

    Two FEL facilities of the FELI are now operating in the wavelength range of 1-20 {mu}m. A 3.2-m hybrid type undulator ({lambda}{sub u}=80mm, N=40) has been designed for far-infrared FELs and will be installed in December. It can cover the wavelength of 20-60 {mu}m by changing K-value from 1 to 2.7 for a 28.0-MeV electron beam. It is composed of ferrite magnetic poles and Sm-Co permanent magnets. Commonly wound coils induce alternating magnetic field in ferrite poles. Combination of the induced field and the permanent magnet field can controls the magnetic field between the undulator gap.

  5. The parasite fauna of stray domestic cats (Felis catus) in Dubai, United Arab Emirates.

    Science.gov (United States)

    Schuster, Rolf K; Thomas, Katja; Sivakumar, Saritha; O'Donovan, Declan

    2009-07-01

    Two hundred forty feral domestic cats trapped between 2004 and 2008 in the city centre and the suburb districts of Dubai, as well as in desert biotopes, were subjected to a complete parasitological dissection. The established parasite fauna consisted of Cystoisospora felis (12.9%), Cystoisospora rivolta (9.2%), Toxoplasma/Hammondia (0.8%), Heterophyes heterophyes (2.5%), Heterophyopsis continua (0.4%), Joyeuxiella spp. (65.8%), Diplopylidium noelleri (37.1%), Hydatigera taeniaeformis (16.7%), Taenia hydatigena (0.4%), Ancylostoma ceylanicum (8.8%), Ollulanus tricuspis (0.8%), Toxocara mystax (2.9%), Toxascaris leonina (0.8%), Pterygodermatites affinis (35.0%), Centrorhynchus aluconis (4.6%), Rhipicephalus sanguineus (4.2%), Xenopsylla astia (3.8%) and Synosternus pallidus (4.2%).

  6. Prevalencia de enfermedades dentales en gatos (felis catus) de los distritos del cono norte de Lima.

    OpenAIRE

    Grandez, Ricardo; Facultad e Medicina Veterinaria y Zootecnia - Universidad Peruana Cayetano Heredia. Lima.; Guerrero, Heidi; Facultad e Medicina Veterinaria y Zootecnia - Universidad Peruana Cayetano Heredia. Lima

    2014-01-01

    Objetivo: Determinar la prevalencia de las patologías dentales de los gatos (Felis catus) del Cono Norte de Lima atendidos en la Clínica Veterinaria de la Universidad Peruana Cayetano Heredia. Material y métodos: Se evaluaron 200 felinos de 1 a 16 años de edad, sin distinción de raza y sexo. Las variables evaluadas fueron: edad (≥1 a <5, ≥5 a <9, ≥9 años), tipo de alimentación (balanceada, mixta sin hueso y mixta con hueso) y localización de la patología dental. Resultados: Se determinó...

  7. Feeding Behavior Modulates Biofilm-Mediated Transmission of Yersinia pestis by the Cat Flea, Ctenocephalides felis.

    Directory of Open Access Journals (Sweden)

    David M Bland

    2016-02-01

    Full Text Available The cat flea, Ctenocephalides felis, is prevalent worldwide, will parasitize animal reservoirs of plague, and is associated with human habitations in known plague foci. Despite its pervasiveness, limited information is available about the cat flea's competence as a vector for Yersinia pestis. It is generally considered to be a poor vector, based on studies examining early-phase transmission during the first week after infection, but transmission potential by the biofilm-dependent proventricular-blocking mechanism has never been systematically evaluated. In this study, we assessed the vector competence of cat fleas by both mechanisms. Because the feeding behavior of cat fleas differs markedly from important rat flea vectors, we also examined the influence of feeding behavior on transmission dynamics.Groups of cat fleas were infected with Y. pestis and subsequently provided access to sterile blood meals twice-weekly, 5 times per week, or daily for 4 weeks and monitored for infection, the development of proventricular biofilm and blockage, mortality, and the ability to transmit. In cat fleas allowed prolonged, daily access to blood meals, mimicking their natural feeding behavior, Y. pestis did not efficiently colonize the digestive tract and could only be transmitted during the first week after infection. In contrast, cat fleas that were fed intermittently, mimicking the feeding behavior of the efficient vector Xenopsylla cheopis, could become blocked and regularly transmitted Y. pestis for 3-4 weeks by the biofilm-mediated mechanism, but early-phase transmission was not detected.The normal feeding behavior of C. felis, more than an intrinsic resistance to infection or blockage by Y. pestis, limits its vector competence. Rapid turnover of midgut contents results in bacterial clearance and disruption of biofilm accumulation in the proventriculus. Anatomical features of the cat flea foregut may also restrict transmission by both early-phase and

  8. Perfil hematológico, bioquímico sérico e sorológico de Felis domesticus com lagochilascariose experimental Hematological, serum biochemical and serological profile of Felis domesticus with experimental lagochilascariosis

    Directory of Open Access Journals (Sweden)

    Mariana Félix de Souza Prudente

    2008-10-01

    Full Text Available No presente trabalho, avaliou-se o hemograma, diversas proteínas e enzimas séricas ou plasmáticas e a produção de anticorpos específicos em Felis domesticus, experimentalmente infectados por Lagochilascaris minor. Verificou-se nos animais infectados aumento de leucócitos totais, principalmente eosinófilos; queda do número de plaquetas; aumento de aspartato-aminotransferase e alanina-aminotransferase; e principalmente a presença de anticorpos IgG específicos para antígenos do parasita. A reação com extrato bruto de parasitas adultos mostrou-se mais específica, permitindo a discriminação de soros de animais: não infectados, com infecção por outros parasitas, e com lagochilascariose. Esta é a primeira descrição da padronização de uma reação sorológica para diagnóstico da lagochilascariose em Felis domesticus.The present study evaluated the hemogram, different proteins, plasma enzymes, serum enzymes and specific antibody production of Felis domesticus experimentally infected by Lagochilascaris minor. The infected animals were seen to present increased total leukocytes (particularly eosinophils, decreased platelet counts, increased aspartate-aminotransferase and alanine-aminotransferase and, especially, the presence of specific IgG antibodies against antigens of the parasite. The reaction with crude extract of adult parasites was shown to be more specific, thereby enabling serum discrimination between the animals: non-infected, infected with other parasites and infected with lagochilascariosis. This is the first description of the standardization of a serological reaction for diagnosing lagochilascariosis in Felis domesticus.

  9. Aspectos morfométricos do timo em gatos domésticos (Felis domesticus Morphometric aspects of the thymus in domestic cats (Felis domesticus

    Directory of Open Access Journals (Sweden)

    Camila E. Barroso

    2012-12-01

    Full Text Available O timo é um órgão linfático primário que desenvolve sua atividade em organismos jovens. Apesar de sua função ser responsável por mecanismos fundamentais na aquisição das defesas e conseqüentes respostas orgânicas, ela ainda não está totalmente esclarecida, nem tampouco as bases morfológicas que respondem por tais funções, como o processo de desenvolvimento e involução do órgão. Objetivou-se analisar e caracterizar os aspectos morfológicos do timo, tais como seu tamanho e volume, e aspectos histológicos do timo em gatos, correlacionando o sexo e o desenvolvimento etário. Doze timos provenientes de fetos de gatos domésticos (Felis domesticus sem raça definida (SRD, machos e fêmeas, separados em três grupos etários. O timo apresentou-se com uma coloração rosa-pálida e com duas porções, a torácica e a cervical, sendo que cada uma delas possuía um lobo direito e um lobo esquerdo em sua maioria. A porção torácica localizava-se em região de mediastino cranial, entre os pulmões e à base do coração. E a porção cervical estendia-se além das costelas em sentido cranial, estando localizada ventralmente à traqueia. A estrutura celular do timo demonstrou-se organizada com a presença de agregados concêntricos, os chamados corpúsculos tímicos, formados por células epiteliais, sustentada por uma cápsula de tecido conjuntivo de onde partiam septos que ao penetrar no órgão dividia-o em lóbulos. Ocorreram variações significativas quanto à lobação e as dimensões do timo entre indivíduos da mesma faixa etária, e entre sexos diferentes. Os valores relativos ao comprimento, espessura e largura, de maneira geral, apresentaram aumento, em conformidade ao desenvolvimento dos animais, mas com diferenças entre os sexos.The thymus is a primary lymphatic organ that develops its activity in young organisms. But despite its function is fundamental mechanisms responsible for the acquisition and subsequent body

  10. Design of FELiChEM, the first infrared free-electron laser user facility in China

    Science.gov (United States)

    Li, He-Ting; Jia, Qi-Ka; Zhang, Shan-Cai; Wang, Lin; Yang, Yong-Liang

    2017-01-01

    FELiChEM is a new experimental facility under construction at the University of Science and Technology of China (USTC). Its core device is two free electron laser oscillators generating middle-infrared and far-infrared laser and covering the spectral range of 2.5-200 μm. It will be a dedicated infrared light source aiming at energy chemistry research. We present the brief design of the FEL oscillators, with the emphasis put on the middle-infrared oscillator. Most of the basic parameters are determined and the anticipated performance of the output radiation is given. The first light of FELiChEM is targeted for the end of 2017. Supported by National Natural Science Foundation of China (21327901)

  11. Interspecies discrimination of A. fumigatus and siblings A. lentulus and A. felis of the Aspergillus section Fumigati using the AsperGenius(®) assay.

    Science.gov (United States)

    Chong, G M; Vonk, A G; Meis, J F; Dingemans, G J H; Houbraken, J; Hagen, F; Gaajetaan, G R; van Tegelen, D W E; Simons, G F M; Rijnders, B J A

    2017-03-01

    The AsperGenius(®) assay detects several Aspergillus species and the A. fumigatus Cyp51A mutations TR34/L98H/T289A/Y121F that are associated with azole resistance. We evaluated its contribution in identifying A. lentulus and A. felis, 2 rare but intrinsically azole-resistant sibling species within the Aspergillus section Fumigati. Identification of these species with conventional culture techniques is difficult and time-consuming. The assay was tested on (i) 2 A. lentulus and A. felis strains obtained from biopsy proven invasive aspergillosis and (ii) control A. fumigatus (n=3), A. lentulus (n=6) and A. felis species complex (n=12) strains. The AsperGenius(®) resistance PCR did not detect the TR34 target in A. lentulus and A. felis in contrast to A. fumigatus. Melting peaks for L98H and Y121F markers differed and those of the Y121F marker were particularly suitable to discriminate the 3 species. In conclusion, the assay can be used to rapidly discriminate A. fumigatus, A. lentulus and A. felis.

  12. Performance of an undulator for visible and UV FELs at FELI

    Energy Technology Data Exchange (ETDEWEB)

    Miyauchi, Y.; Zako, A.; Koga, A. [Free Electron Laser Research Institute, Inc., Osaka (Japan)] [and others

    1995-12-31

    Two infrared free electron lasers (FELs) of the FELI project are now operating in the wavelength range of 1-20{mu}m. A 2.68-m undulator has been constructed for visible and UV FELs covering the wavelength of 1-0.2{mu}m for 100-165 MeV electron beams. It generates alternating, horizontal magnetic field, and wiggles electron beam on a vertical plane. The undulator length and period are 2.68m and 40mm, respectively. The gap of undulator magnets can be changed remotely by using servomotors with an accuracy of 1 {mu}m from the control room. The maximum K-value and related magnetic field strength are 1.9 and 0.5T, respectively, when its gap is set to the minimum value of 16mm. In order to minimize magnetic field reduction due to radiation damage, Sm-Co permanent magnet was adopted. Its structure and the results of magnetic field measurement will be reported.

  13. Feeding habits of feral cats Felis silvestris catus in the countryside of Majorca Island, Spain

    Directory of Open Access Journals (Sweden)

    J. Milan

    2010-06-01

    Full Text Available The diet of feral cats ( Felis silvestris catus in the Mediterranean island of Majorca (Spain was studied from July 2008 to June 2009 by the analysis of the scats of 75 feral cats captured in baited traps in 14 different areas. A total of 138 preys were identified in the analyzed scats. Mammals were the main group preyed on and constituted 93% both in frequency and biomass. Among them, mice were the most frequent prey consumed (55% Mus sp., 18% Apodemus sylvaticus but represented only 20% of biomass. Rat was present in 29% of scats and was the main component in terms of biomass (57%. Rabbit was found at a frequency of 6.6%, and constituted 18% of biomass. Other prey (birds, geckos and insects were found in lower frequency, and all pooled constituted only 7% of biomass. Reproductive females preyed less upon mice (20% than the other cats (77%. This may indicate that these females tended to predate upon higher preys, which may be secondary to increased energetic requirements due to pregnancy or lactation. No seasonal variations were found in any of the different parameters studied. Results indicate that rodents constitute all year round the main prey item in feral cat diet in the countryside of Majorca.

  14. Mesothelioma in Two Nondomestic Felids: North American Cougar (Felis concolor and Cheetah (Acinonyx jubatus

    Directory of Open Access Journals (Sweden)

    Amanda Whiton

    2013-01-01

    Full Text Available A 15-year-old male North American cougar (Felis concolor presented with a 2-day history of anorexia, restlessness, and dyspnea. White blood cell count ( cells/μL and absolute segmented neutrophil count ( cells/μL were increased, and BUN (143 mg/dL, creatinine (6.3 mg/dL, and phosphorus (8.5 mg/dL concentrations indicated chronic renal disease. Thoracic radiographs showed severe pleural and pericardial effusion. During attempts to remove the fluid, cardiac tamponade developed and the cat died. At necropsy, nodular masses decorated the pericardium at the level of the base of the heart. The final microscopic diagnosis was mesothelioma of the pericardium, tunica adventitia of the main pulmonary artery, left auricle epicardium, and left ventricular epicardium. A 15-year-old female cheetah (Acinonyx jubatus was evaluated for acute respiratory distress. The white blood cell count ( cells/μL and absolute segmented neutrophil count ( cells/μL were increased. Radiographically pleural effusion and a cranial thoracic mass were seen. The cheetah was euthanized, and a gross diagnosis of disseminated pleural mesothelioma with thoracic effusion was made. Histologically, pleural mesothelioma was confirmed with local invasion of the lung and pulmonary arterial emboli and infarction. In both cases, a diagnosis of mesothelioma was made based on cellular morphology, microscopic architecture, and neoplastic cell coexpression of cytokeratin and vimentin.

  15. Changes in selected hematology and serum biochemistry in Turkish Angora cats (Felis catus during growth period

    Directory of Open Access Journals (Sweden)

    Ozkan Simsek

    2015-03-01

    Full Text Available The purpose of the present study was to determine the changes in selected hematology and serum biochemistry of Angora cats (Felis catus during growth period. A total of 32 Angora cats (16 adults and 16 kittens were used in this study. Blood samples were collected from the animals, and were analyzed for white blood cells, red blood cells, hemoglobin, packed cell volume, mean corpuscular volume (MCV, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, granulocytes, monocytes and lymphocytes numbers. In the serum, alanine aminotransferase, aspartate aminotransferase (AST, alkaline phosphatase (ALP, gamma glutamyl transferase (GGT, lactate dehydrogenase (LDH, creatinine kinase (CK, total cholesterol, glucose, triglyceride, urea, creatinine, total protein, albumin, Ca, Mg, Pi levels were determined. Monocyte level was found higher, and ALP, LDH, CK activities and Pi levels were lower in adult cats as compared to the kittens. MCV was lower and GGT and AST activities, and glucose level were higher in kittens of 1.5-3 months old than in kittens of >3 months. Concentrations of total cholesterol and Mg were higher in kitten (1.5-3 months old than in adult cats. In conclusion, age related effects on hematological and biochemical blood parameters have been determined for the first time in Angora cats.

  16. Gastrointestinal Helminthic Parasites in Stray Cats (Felis catus from North of Iran

    Directory of Open Access Journals (Sweden)

    A Rezaei-Doust

    2007-08-01

    Full Text Available Background: Cats play a crucial role in the epidemiology of gastrointestinal helminthic parasites and also play a major role in transmitting of these parasites through faecal contamination of soil, food or water. The aim of this study was to determine the species of gastrointestinal helminthes parasites in stray cats from a rural area of Bandar-e-Anzali, Iran.Method: Gastrointestinal helminthes were collected from 50 necropsied stray cats (Felis catus after capturing them by trapping from different regions of the city and humanely euthanatized in Bandar-e-Anzali, a port in the Caspian Sea in northern Iran, from March to November 2003. Results: The prevalence of infection was 90%, with those of individual parasites being Diplopylidium nolleri 54%, Phy­saloptera praeputialis 32%, Ancylostoma tubaeforme 20%, Joyeuxiella pasqualei 10%, Toxocara cati 8%, Pterygoderma­tites affinis 6%, Ancylostoma caninum 4%, and Taenia taeniaeformis 2%. Concurrent infections with two or more parasites were recorded in 34% of the individuals. In relation to the sex, the differences were not significant. Conclusion: P. praeputialis, T. cati, D. nolleri and sometime J. pasqualei are the commonest Helminthes in cats. This is the first reported isolation of P. affinis and A. caninum infections from cats in Iran.

  17. Detection of Rickettsia in Rhipicephalus sanguineus ticks and Ctenocephalides felis fleas from southeastern Tunisia by reverse line blot assay.

    Science.gov (United States)

    Khrouf, Fatma; M'Ghirbi, Youmna; Znazen, Abir; Ben Jemaa, Mounir; Hammami, Adnene; Bouattour, Ali

    2014-01-01

    Ticks (n = 663) and fleas (n = 470) collected from domestic animals from southeastern Tunisia were screened for Rickettsia infection using reverse line blot assay. Evidence of spotted fever group Rickettsia was obtained. We detected Rickettsia felis in fleas, Rickettsia massiliae Bar 29 and the Rickettsia conorii Israeli spotted fever strain in ticks, and Rickettsia conorii subsp. conorii and Rickettsia spp. in both arthropods. The sensitivity of the adopted technique allowed the identification of a new association between fleas and R. conorii subsp. conorii species. The presence of these vector-borne Rickettsia infections should be considered when diagnosing this disease in humans in Tunisia.

  18. Phenotypic and Molecular Characterization of Domestic Cat (Felis catus) Spermatogonial Stem Cells.

    Science.gov (United States)

    Powell, Robin H; Galiguis, Jason; Biancardi, Monica N; Pope, C Earle; Leibo, Stanley P; Wang, Guoshun; Gómez, Martha C

    2016-07-01

    In many mammalian species, surface markers have been used to obtain enriched populations of spermatogonial stem cells (SSCs) for assisted reproduction and other applications; however, little is known about the expression patterns of feline SSCs. In this study, we assessed expression of the SSC surface markers commonly used in other species, KIT, ITGA6, CD9, GFRalpha1, ADGRA3, and THY1, in addition to the less frequently used pluripotent markers TRA-1-60, TRA-1-81, SSEA-1, and SSEA-4 in SSCs of both prepubertal and adult domestic cats (Felis catus). To further characterize cat SSCs, we sorted cells using SSC-specific markers and evaluated the expression of the pluripotent transcription factors NANOG, POU5F1, and SOX2 and the proto-oncogene MYC within these populations. We concluded that SSC surface markers used in other mammalian species were not specific for identifying cat SSCs. However, the pluripotent markers we evaluated were more specific to cat spermatogonia, and the presence of SSEA-1 and SSEA-4 in fewer and primarily individual cells suggests that these two markers may be used for enrichment of cat SSCs. The expression of pluripotent transcription factors at mRNA level by single-stained cells positive for SSEA-4 and by dual-stained cells positive for both GFRalpha1 and SSEA-4 reflects the undifferentiated stage of cat SSCs. The absence of transcription factors in double-stained cells positive for only one marker implies the loss of the stem cell-like identity with the loss of either GFRalpha1 or SSEA-4. Further investigation is warranted to elucidate the biological characteristics of these spermatogonial subpopulations.

  19. Survey on parasitic infections in wildcat (Felis silvestris silvestris Schreber, 1777) by scat collection.

    Science.gov (United States)

    Napoli, Ettore; Anile, Stefano; Arrabito, Carmelo; Scornavacca, Davide; Mazzamuto, Maria Vittoria; Gaglio, Gabriella; Otranto, Domenico; Giannetto, Salvatore; Brianti, Emanuele

    2016-01-01

    Wildcats are endangered felid species living in Europe, Asia, and Africa. Regrettably, scientific information on parasites of wildcats is particularly meager and they often rely on data gained by necropsies of a small number of animals. In the present study, scat collection was used to assess the parasite spectrum of European wildcats living in the Etna Park (Sicily, Italy). Scat collection was performed from May to September 2010 by weekly walking four transects for a total of 391 km. Samples were then analyzed by flotation and sedimentation techniques to investigate wildcat parasitic fauna. A total of 121 scats of wildcats were collected, and parasitic forms (i.e., oocysts, eggs, and larvae) were retrieved in 110 (90.9 %) of the samples. Parasites found were Physaloptera sp. (52.1 %), tapeworms (45.5 %), Toxocara cati (43.8 %), Eucoleus aerophilus (27.3 %), Ancylostoma sp. (22.3 %), Troglostrongylus brevior (15.7 %), trematodes (9.9 %), Isospora felis (4.1 %), Cylicospirura sp. (1.7 %), and Acanthocephala (0.8 %). The prevalence of endoparasitic infections herein recorded is similar to that described in other studies conducted using necropsy technique. The species richness of parasites found in the present survey, with a total of nine helminths and one protozoon, is the highest ever reported for wildcat in Europe. Scat collection and examination are reliable and rapid non-invasive tools which can be used in a systematic survey design to study the parasite spectrum of wildcat as well as that of other endangered wild species.

  20. Functional Analyses of Bitter Taste Receptors in Domestic Cats (Felis catus.

    Directory of Open Access Journals (Sweden)

    Weiwei Lei

    Full Text Available Cats are obligate carnivores and under most circumstances eat only animal products. Owing to the pseudogenization of one of two subunits of the sweet receptor gene, they are indifferent to sweeteners, presumably having no need to detect plant-based sugars in their diet. Following this reasoning and a recent report of a positive correlation between the proportion of dietary plants and the number of Tas2r (bitter receptor genes in vertebrate species, we tested the hypothesis that if bitter perception exists primarily to protect animals from poisonous plant compounds, the genome of the domestic cat (Felis catus should have lost functional bitter receptors and they should also have reduced bitter receptor function. To test functionality of cat bitter receptors, we expressed cat Tas2R receptors in cell-based assays. We found that they have at least 7 functional receptors with distinct receptive ranges, showing many similarities, along with some differences, with human bitter receptors. To provide a comparative perspective, we compared the cat repertoire of intact receptors with those of a restricted number of members of the order Carnivora, with a range of dietary habits as reported in the literature. The numbers of functional bitter receptors in the terrestrial Carnivora we examined, including omnivorous and herbivorous species, were roughly comparable to that of cats thereby providing no strong support for the hypothesis that a strict meat diet influences bitter receptor number or function. Maintenance of bitter receptor function in terrestrial obligate carnivores may be due to the presence of bitter compounds in vertebrate and invertebrate prey, to the necessary role these receptors play in non-oral perception, or to other unknown factors. We also found that the two aquatic Carnivora species examined had fewer intact bitter receptors. Further comparative studies of factors driving numbers and functions of bitter taste receptors will aid in

  1. An insight into the sialotranscriptome of the cat flea, Ctenocephalides felis.

    Directory of Open Access Journals (Sweden)

    José M C Ribeiro

    Full Text Available BACKGROUND: Saliva of hematophagous arthropods contains a diverse mixture of compounds that counteracts host hemostasis. Immunomodulatory and antiinflammatory components are also found in these organisms' saliva. Blood feeding evolved at least ten times within arthropods, providing a scenario of convergent evolution for the solution of the salivary potion. Perhaps because of immune pressure from hosts, the salivary proteins of related organisms have considerable divergence, and new protein families are often found within different genera of the same family or even among subgenera. Fleas radiated with their vertebrate hosts, including within the mammal expansion initiated 65 million years ago. Currently, only one flea species-the rat flea Xenopsylla cheopis-has been investigated by means of salivary transcriptome analysis to reveal salivary constituents, or sialome. We present the analysis of the sialome of cat flea Ctenocephaides felis. METHODOLOGY AND CRITICAL FINDINGS: A salivary gland cDNA library from adult fleas was randomly sequenced, assembled, and annotated. Sialomes of cat and rat fleas have in common the enzyme families of phosphatases (inactive, CD-39-type apyrase, adenosine deaminases, and esterases. Antigen-5 members are also common to both sialomes, as are defensins. FS-I/Cys7 and the 8-Cys families of peptides are also shared by both fleas and are unique to these organisms. The Gly-His-rich peptide similar to holotricin was found only in the cat flea, as were the abundantly expressed Cys-less peptide and a novel short peptide family. CONCLUSIONS/SIGNIFICANCE: Fleas, in contrast to bloodsucking Nematocera (mosquitoes, sand flies, and black flies, appear to concentrate a good portion of their sialome in small polypeptides, none of which have a known function but could act as inhibitors of hemostasis or inflammation. They are also unique in expansion of a phosphatase family that appears to be deficient of enzyme activity and has an

  2. Molecular characterisation of Cryptosporidium and Giardia in cats (Felis catus) in Western Australia.

    Science.gov (United States)

    Yang, Rongchang; Ying, Joyce Lau Jie; Monis, Paul; Ryan, Una

    2015-08-01

    Little is known of the prevalence of Cryptosporidium and Giardia in domestic cats in Western Australia and their potential role as zoonotic reservoirs for human infection. In the present study, a total of 345 faecal samples from four different sources were screened for the presence of Cryptosporidium and Giardia by PCR and genotyped by sequence analysis. Oocyst numbers and cyst numbers for Cryptosporidium and Giardia respectively were also determined using quantitative PCR assays. Cryptosporidium and Giardia were detected in 9.9% (95% CI 6.7-13.0) and 10.1% (95% CI 7.0-13.3) of cats in Western Australia respectively. Sequence analysis at the 18S rRNA locus identified five Cryptosporidium species/genotypes; C. felis (n = 8), C. muris (n = 1), C. ryanae (n = 1), Cryptosporidium rat genotype III (n = 5) and a novel genotype most closely related to Cryptosporidium rat genotype III in one isolate. This is the first report of C. ryanae and Cryptosporidium rat genotype III in cats. For Giardia, assemblage F the most commonly identified species, while only 1 assemblage sequence was detected. Since most human cases of cryptosporidiosis are caused by C. parvum and C. hominis and human cases of giardiasis are caused by G. duodenalis assemblage A and B, the domestic cats in the present study are likely to be of low zoonotic risk to pet owners in Perth. Risk analyses identified that elderly cats (more than 6 years) were more prone to Cryptosporidium and Giardia infections than kittens (less than 6 months) (P = 0.009). Clinical symptoms were not associated with the prevalence of Cryptosporidium and Giardia infections in cats.

  3. Frequent detection of transcriptionally active Felis catus papillomavirus 2 in feline cutaneous squamous cell carcinomas.

    Science.gov (United States)

    Thomson, Neroli A; Munday, John S; Dittmer, Keren E

    2016-05-01

    Felis catus papillomavirus 2 (FcaPV-2) causes premalignant skin lesions in cats and has also been found in a proportion of cutaneous squamous cell carcinomas (SCCs) - a common and potentially fatal cancer of cats. Whilst this could suggest a role of the virus in cancer development, FcaPV-2 has also been detected in skin swabs of normal cats, making it difficult to discern whether the papillomavirus is causing the cancer or merely an 'innocent bystander'. To distinguish between these two possibilities, real-time PCR was used to determine the viral copy number and the transcriptional activity of FcaPV-2 infections present in 70 formalin-fixed paraffin-embedded skin lesions including 10 papillomavirus-induced premalignant lesions and 60 SCCs. FcaPV-2 gene expression was found in 21 of 60 (35 %) SCCs, all 10 premalignant lesions and none of 10 normal skin samples. The results showed two distinct subsets of SCCs. The majority of the SCCs had low copy numbers of FcaPV-2 DNA (mean of 17 copies per copy of reference gene DNA) and no FcaPV-2 gene expression, suggesting the virus was an incidental finding. In contrast, 20 SCCs had detectable FcaPV-2 E6/E7 gene expression and very high copy numbers of FcaPV-2 DNA, with a mean of 32 930 copies per copy of reference gene DNA. The relative quantity of E6/E7 gene expression and the viral copy number in this group were similar to those found in the papillomavirus-induced premalignant lesions, suggesting that FcaPV-2 may play a role in the development of a subset of feline cutaneous SCCs.

  4. Extent of linkage disequilibrium in the domestic cat, Felis silvestris catus, and its breeds.

    Directory of Open Access Journals (Sweden)

    Hasan Alhaddad

    Full Text Available Domestic cats have a unique breeding history and can be used as models for human hereditary and infectious diseases. In the current era of genome-wide association studies, insights regarding linkage disequilibrium (LD are essential for efficient association studies. The objective of this study is to investigate the extent of LD in the domestic cat, Felis silvestris catus, particularly within its breeds. A custom illumina GoldenGate Assay consisting of 1536 single nucleotide polymorphisms (SNPs equally divided over ten 1 Mb chromosomal regions was developed, and genotyped across 18 globally recognized cat breeds and two distinct random bred populations. The pair-wise LD descriptive measure (r(2 was calculated between the SNPs in each region and within each population independently. LD decay was estimated by determining the non-linear least-squares of all pair-wise estimates as a function of distance using established models. The point of 50% decay of r(2 was used to compare the extent of LD between breeds. The longest extent of LD was observed in the Burmese breed, where the distance at which r(2 ≈ 0.25 was ∼380 kb, comparable to several horse and dog breeds. The shortest extent of LD was found in the Siberian breed, with an r(2 ≈ 0.25 at approximately 17 kb, comparable to random bred cats and human populations. A comprehensive haplotype analysis was also conducted. The haplotype structure of each region within each breed mirrored the LD estimates. The LD of cat breeds largely reflects the breeds' population history and breeding strategies. Understanding LD in diverse populations will contribute to an efficient use of the newly developed SNP array for the cat in the design of genome-wide association studies, as well as to the interpretation of results for the fine mapping of disease and phenotypic traits.

  5. Redescription of Parspina argentinensis (Szidat, 1954) (Digenea: Cryptogonimidae) from freshwater fishes (Pimelodidae) in the basins of the Paraná and La Plata Rivers, Argentina, with comments on P. bagre Pearse, 1920.

    Science.gov (United States)

    Ostrowski de Núñez, Margarita C; Arredondo, Nathalia J; Doma, Irene L; Gil de Pertierra, Alicia A

    2011-01-01

    The type-specimens of Parspina argentinensis (Szidat, 1954) from Pimelodus maculatus Lacépède, and new material from Iheringichthys labrosus (Lütken), Parapimelodus valenciennis (Lütken), Pimelodella gracilis (Valenciennes), Pimelodus albicans (Valenciennes), P. argenteus Perugia and P. maculatus caught in the basins of the La Plata and Paraná Rivers, Argentina, were studied to elucidate its taxonomic status. The type-specimens of Parspina bagre Pearse, 1920 from Pimelodella metae Eigenmann in Lake Valencia, Venezuela, were also studied and new observations on its morphology recorded. The amendation of the generic diagnosis of Parspina Pearse, 1920 is proposed based on the study of both species, which revealed, among other features, the absence of a thin walled-membrane enclosing the male terminal genitalia. Parspina argentinensis is characterised by the following features: absence of a gonotyl; presence of a bipartite seminal vesicle, pars prostatica and ejaculatory duct; caeca of nearly equal length; uterus extending from the level of the ventral sucker to end of body; testes symmetrical to slightly oblique; ovary transversely elongate, compact and variable in shape; and the utilisation of a wide range of freshwater fishes as hosts. The tegumental surface of this species is covered with pectinate spines arranged quincuncially. Spines decrease in size and density from forebody to hindbody. There are two types of sensory papillae, ciliated and dome-shaped. Ciliated papillae are distributed on the surface of the oral region and on the dorsal and ventral surfaces of the body, whereas dome-shaped papillae are found on the surface of the ventral rim of the oral sucker associated with a ciliated papilla. Gland-duct openings are interspersed with the spines of the oral crown. In addition, the infection indices of P. argentinensis vary widely, not only among different hosts but also between the Paraná and La Plata River basins. Higher prevalences of P. argentinensis

  6. Biología reproductiva del bagre cominate Occidentarius platypogon (Pisces: Ariidae en el sureste del golfo de California Reproductive biology of the cominate sea catfish Occidentarius platypogon (Pisces: Ariidae from the southeastern Gulf of California

    Directory of Open Access Journals (Sweden)

    Felipe Amezcua

    2012-07-01

    Full Text Available En el sureste del golfo de California, el bagre cominate presenta gran abundancia y es explotado comercialmente. Sin embargo, estudios sobre su biología son escasos y no existen normas que regulen su explotación en México. En este trabajo se describe la biología reproductiva de Occidentarius platypogon. Se capturaron 480 organismos con redes agalleras entre octubre 2008 y diciembre 2009. El estado de madurez gonadal se determinó macroscópicamente. Esta especie tiene una época de desove de mayo a agosto con un desarrollo sincrónico en dos lotes. La fecundidad media fue de 37 ovocitos maduros. La baja fecundidad se debe a que esta especie presenta cuidado parental con incubación oral por parte de los machos, lo cual reduce su mortalidad natural en las primeras etapas de desarrollo. La talla de primera madurez fue de 37,6 cm y la longitud estimada de primera captura de la pesquería artesanal con redes de enmalle fue de 23,4 cm, lo que indica la captura de organismos que aún no alcanzan su talla reproductiva. La mayoría de los organismos capturados fueron machos incubando, por lo tanto, podría ser alta la mortalidad por pesca de juveniles.The cominate sea catfish from the southeastern Gulf of California is an important species that is commercially exploited. However, studies on biology of this species are scarce; therefore, harvest regulations do not exist for this species in Mexico. The aim of this work was to describe reproductive biology of Occidentarius platypogon. Gillnet samples were carried out from October 2008 to December 2009. In total, 480 fish were dissected; gender sex and gonad maturity stage were determined macroscopically. Results indicated that the spawning season runs from May to August and generally with synchronic gonad development. Fecundity of the chihuil sea catfish was 37, which is low because this species shows parental care via oral incubation of fry, reducing mortality. Estimated length at first maturity is 37

  7. Caracal depereti nov. Sp. Y felis aff. Silvestris (felidae, mammalia del plioceno inferior de layna (Soria, España

    Directory of Open Access Journals (Sweden)

    Salesa, M. J.

    2003-08-01

    Full Text Available Two species of small-medium size felids, Felis aff. silvestris and Caracal depereti nov. sp., have been identified from the Pliocene karstic locality of Layna (Soria, Spain. Caracal depereti nov. sp. shows close affinities with Caracal issiodorensis, species that has been traditionally classified in the genus Lynx. This new interpretation implies that there are no evidences of lynxes in the Pliocene of Westem Europe, and probably this consideration can be applicable to other Eurasiatic localities where Lynx issiodorensis has been determined.Dos especies de félidos de talla pequeña a media, Felis aff. silvestris y Caracal depereti nov. sp., han sido identificadas en el yacimiento cárstico plioceno de Layna, Soria. Caracal depereti nov. sp. muestra estrechas afinidades con Caracal issiodorensis, especie que tradicionalmente ha sido clasificada en el género Lynx. Esta interpretación implica que no hay evidencias de linces en el Plioceno de Europa occidental, y probablemente esta consideración pueda aplicarse a otros yacimientos pliocenos de Eurasia, en los que se ha determinado Lynx issiodorensis.

  8. Assessment of the onset of action of afoxolaner against existing adult flea (Ctenocephalides felis) infestations on dogs.

    Science.gov (United States)

    Kunkle, Bruce N; Drag, Marlene D; Chester, Theodore S; Larsen, Diane L

    2014-04-02

    The speed of kill of afoxolaner against experimental infestations by Ctenocephalides felis was evaluated after oral administration of afoxolaner in a soft chew (NEXGARD(®)) at a dose to achieve 2.5mg/kg bodyweight. Forty beagles were allocated to two treatment groups. Dogs in Treatment Group 1 were untreated controls. Dogs in Treatment Group 2 were treated on Day-0 with afoxolaner, according to their pre-treatment bodyweight. All dogs were infested with approximately 100 C. felis on Day-1. Live fleas were counted upon removal at 5 time points after treatment (i.e., 2, 4, 8, 12 and 24h after treatment). For each time point, counts were performed on 4 dogs from each of the treated and the untreated groups. Early curative flea killing efficacy was evaluated with respect to the untreated control group. The afoxolaner treated group had significantly fewer fleas than the untreated control group at 8, 12, and 24h (pafoxolaner were 15.0%, 87.8%, 99.5%, 100.0%, and 100.0% at 2, 4, 8, 12, and 24h, respectively. In this study, afoxolaner began killing fleas by 2h after treatment with increasing efficacy at subsequent time points and had >99.5% efficacy at 8, 12, and 24h after treatment demonstrating an early onset of action.

  9. Toxoplasmosis in sand cats (Felis margarita) and other animals in the Breeding Centre for Endangered Arabian Wildlife in the United Arab Emirates

    Science.gov (United States)

    The Sand cat (Felis margarita) is a small-sized felid occurring in the United Arab Emirates (UAE). The Sand cat captive breeding program at the Breeding Centre for Endangered Arabian Wildlife (BCEAW), Sharjah, UAE, has experienced high newborn mortality rates, and congenital toxoplasmosis was recent...

  10. Interspecies discrimination of A. fumigatus and siblings A. lentulus and A. felis of the Aspergillus section Fumigati using the AsperGenius(®) assay

    NARCIS (Netherlands)

    Chong, G M; Vonk, A G; Meis, J F; Dingemans, G J H; Houbraken, J; Hagen, F.; Gaajetaan, G R; van Tegelen, D W E; Simons, G F M; Rijnders, B J A

    2016-01-01

    The AsperGenius(®) assay detects several Aspergillus species and the A. fumigatus Cyp51A mutations TR34/L98H/T289A/Y121F that are associated with azole resistance. We evaluated its contribution in identifying A. lentulus and A. felis, 2 rare but intrinsically azole-resistant sibling species within t

  11. Interspecies discrimination of A. fumigatus and siblings A. lentulus and A. felis of the Aspergillus section Fumigati using the AsperGenius® assay

    NARCIS (Netherlands)

    G.M. Chong; A.G. Vonk (Alieke); J.F. Meis (Jacques F.); G. Dingemans (Gijs); J. Houbraken (Jos); F. Hagen (Ferry); G.R. Gaajetaan (Giel R.); D.W.E. Van Tegelen (Dennis W. E.); G.F.M. Simons (Guus F. M.); B.J.A. Rijnders (Bart)

    2016-01-01

    textabstractThe AsperGenius® assay detects several Aspergillus species and the A. fumigatus Cyp51A mutations TR34/L98H/T289A/Y121F that are associated with azole resistance. We evaluated its contribution in identifying A. lentulus and A. felis, 2 rare but intrinsically azole-resistant sibling specie

  12. Aspectos morfométricos do timo em gatos domésticos (Felis domesticus

    Directory of Open Access Journals (Sweden)

    Camila E. Barroso

    2012-12-01

    Full Text Available O timo é um órgão linfático primário que desenvolve sua atividade em organismos jovens. Apesar de sua função ser responsável por mecanismos fundamentais na aquisição das defesas e conseqüentes respostas orgânicas, ela ainda não está totalmente esclarecida, nem tampouco as bases morfológicas que respondem por tais funções, como o processo de desenvolvimento e involução do órgão. Objetivou-se analisar e caracterizar os aspectos morfológicos do timo, tais como seu tamanho e volume, e aspectos histológicos do timo em gatos, correlacionando o sexo e o desenvolvimento etário. Doze timos provenientes de fetos de gatos domésticos (Felis domesticus sem raça definida (SRD, machos e fêmeas, separados em três grupos etários. O timo apresentou-se com uma coloração rosa-pálida e com duas porções, a torácica e a cervical, sendo que cada uma delas possuía um lobo direito e um lobo esquerdo em sua maioria. A porção torácica localizava-se em região de mediastino cranial, entre os pulmões e à base do coração. E a porção cervical estendia-se além das costelas em sentido cranial, estando localizada ventralmente à traqueia. A estrutura celular do timo demonstrou-se organizada com a presença de agregados concêntricos, os chamados corpúsculos tímicos, formados por células epiteliais, sustentada por uma cápsula de tecido conjuntivo de onde partiam septos que ao penetrar no órgão dividia-o em lóbulos. Ocorreram variações significativas quanto à lobação e as dimensões do timo entre indivíduos da mesma faixa etária, e entre sexos diferentes. Os valores relativos ao comprimento, espessura e largura, de maneira geral, apresentaram aumento, em conformidade ao desenvolvimento dos animais, mas com diferenças entre os sexos.

  13. Space and Habitat Selection by Female European Wild Cats (Felis silvestris silvestris

    Directory of Open Access Journals (Sweden)

    Tarroso, P.

    2006-12-01

    Full Text Available Studies on the use of space and habitat selection of threatened species are useful for identifying factors that influence fitness of individuals and population viability. However, there is a considerable lack of published information regarding these factors for the European wildcat (Felis silvestris. Serra da Malcata Nature Reserve (SMNR, a mountainous area in the eastern centre of Portugal, hosts a stable wildcat population which constitutes a priority in terms of conservation. We studied space use and habitat selection of female wildcats in SMNR with the following objectives: 1 to describe seasonal space use and habitat selection and 2 to obtain information on priority habitats for wildcats in order to develop a proper conservation strategy. We used radio-telemetry as the basic tool for our study and we analysed habitat selection using an Euclidean distance-based approach to investigate seasonal and annual habitat selection by wildcats. We detected that during spring females exhibit smaller home ranges and core areas. Females exhibited habitat selection for establishing home ranges from the available habitats within the study area. In fact, females selected Quercus pyrenaica forests and Quercus rotundifolia and Arbutus unedo forests positively and avoided Erica spp. and Cistus ladanifer scrubland and other habitats. Quercus pyrenaica forests and Quercus rotundifolia and Arbutus unedo forests are important habitats for female wildcats because they provide shelter and food resources, such as small mammals. They also contain elevated tree cavities which can be use as dens. In contrast, Erica spp. and Cistus ladanifer scrubland is an extremely dense habitat with low associated biodiversity and so wildcats avoid it. We believe that this habitat, as well as pine stands, do not provide food and cover resources for wildcats. Home ranges with higher percentage of these habitat types tend to be larger, since females are required to use larger areas to

  14. Prevalence of Bartonella species, hemoplasmas, and Rickettsia felis DNA in blood and fleas of cats in Bangkok, Thailand.

    Science.gov (United States)

    Assarasakorn, S; Veir, J K; Hawley, J R; Brewer, M M; Morris, A K; Hill, A E; Lappin, M R

    2012-12-01

    Flea infestations are common in Thailand, but little is known about the flea-borne infections. Fifty flea pools and 153 blood samples were collected from client-owned cats between June and August 2009 from veterinary hospitals in Bangkok, Thailand. Total DNA was extracted from all samples, and then assessed by conventional PCR assays. The prevalence rates of Bartonella spp. in blood and flea samples were 17% and 32%, respectively, with DNA of Bartonella henselae and Bartonella clarridgeiae being amplified most commonly. Bartonella koehlerae DNA was amplified for the first time in Thailand. Hemoplasma DNA was amplified from 23% and 34% of blood samples and flea pools, respectively, with 'Candidatus Mycoplasma haemominutum' and Mycoplasma haemofelis being detected most frequently. All samples were negative for Rickettsia felis. Prevalence rate of B. henselae DNA was increased 6.9 times in cats with flea infestation. Cats administered flea control products were 4.2 times less likely to be Bartonella-infected.

  15. Prevalence of Zoonotic and Other Intestinal Protozoan Parasites in Stray Cats (Felis domesticus of Kerman, South-East of Iran

    Directory of Open Access Journals (Sweden)

    Sakineh BEIGI

    2017-01-01

    Full Text Available Intestinal protozoan parasites constitute a major source of diseases for stray cats and have been recognized as important public health problems in several parts of the world. Considering the potential risk of stray cats for public health, present cross-sectional study was carried out to determine the type and frequency of protozoan parasites by faecal examination. A total of 100 stray cats were examined in Kerman city, Iran, Overall 67 cats (67% were infected with at least one protozoan parasite. The following parasites, with their respective prevalence, were found; Isospora felis 38%, Isospora rivolta 25%, Toxoplasma gondii 16%, Sarcocystis spp. 8%, Cryptosporidium spp. 7%, and Giardia sp. 5%. Based on our data, the sex of stray cats was not significantly associated with the prevalence of gastrointestinal protozoan parasites. The high infection rate of zoonotic intestinal protozoan parasites in stray cats is considered to be critical from the viewpoint of public health importance.We

  16. Genetic variability of Herpailurus yagouaroundi, Puma concolor and Panthera onca (Mammalia, Felidae studied using Felis catus microsatellites

    Directory of Open Access Journals (Sweden)

    Vanessa Roma Moreno

    2006-01-01

    Full Text Available We used four microsatellite loci (Fca08, Fca45, Fca77 and Fca96 from the domestic cat, Felis catus, to investigate genetic variability in specimens of Herpailurus yagouaroundi (jaguarundi, otter cat, eyra, Puma concolor (cougar, mountain lion, puma and Panthera onca (jaguar held in various Brazilian zoos. Samples of DNA from the cats were PCR amplified and then sequenced before being analyzed using the CERVUS program. Our results show a mean polymorphic information content (PIC of 0.83 for H. yagouaroundi, 0.66 for P. concolor and 0.69 for P. onca and a mean of 10.3 alleles for the Fca08 locus, 5.3 for Fca 45, 9 for Fca 77 and 14 for Fca 96. These results indicate a relatively high level of genetic diversity for the specimens studied.

  17. Survival of feral cats, Felis catus (Carnivora: Felidae), on Mauna Kea, Hawai'i, based on tooth cementum lines

    Science.gov (United States)

    Danner, Raymond M.; Farmer, Chris; Hess, Steven C.; Stephens, Robert M.; Banko, Paul C.

    2010-01-01

    Feral cats (Felis catus) have spread throughout anthropogenic and insular environments of the world. They now threaten many species of native wildlife with chronic depredation. Knowledge of feral cat population dynamics is necessary to understand their ecological effects and to develop effective control strategies. However, there are few studies worldwide regarding annual or lifetime survival rates in remote systems, and none on Pacific islands. We constructed the age distribution and estimated survival of feral cats in a remote area of Hawai'i Island using cementum lines present in lower canine teeth. Our data suggest annual cementum line formation. A log-linear model estimated annual survival ≥ 1 yr of age to be 0.647. Relatively high survival coupled with high reproductive output allows individual cats to affect native wildlife for many years and cat populations to rebound quickly after control efforts.

  18. Genomic characterization of Felis catus papillomavirus-3: a novel papillomavirus detected in a feline Bowenoid in situ carcinoma.

    Science.gov (United States)

    Munday, John S; Dunowska, Magda; Hills, Simon F; Laurie, Rebecca E

    2013-08-30

    There is increasing evidence that papillomaviruses (PVs) may cause skin cancer in cats. Neoplasms most frequently contain Felis domesticus PV type 2 (FdPV-2) DNA, but other PV DNA sequences have also been detected suggesting multiple PVs could cause disease. One of these sequences, FdPV-MY2, was previously detected in 5 of a series of 70 feline skin cancers. The aim was to determine the genome sequence of this PV. Using the circular nature of PV DNA, 'outward facing' primers specific for FdPV-MY2 were designed and amplified a 7300 bp length of DNA from a feline Bowenoid in situ carcinoma (BISC) that showed microscopic evidence of a viral etiology and tested positive for FdPV-MY2 DNA. The PCR product was sequenced using next generation sequencing technology. The full genomic sequence of the virus, comprising 7583 bp, was assembled and analyzed. As this is the third PV from a domestic cat, the virus was designated Felis catus PV type 3 (FcaPV-3). Consistent with other PVs, the putative coding regions of FcaPV-3 were predicted to produce 6 early proteins and 2 late ones. Classification was difficult as the virus contained over 60% nucleotide similarity within the ORF L1 with PVs from 3 different genera. However, based on phylogenetic analysis of ORF L1, FcaPV-3 was most closely related to the tau-PVs CPV-2 and CPV-7. As FcaPV-3 has over 60% nucleotide similarity with the ORF L1 of both tau-PVs, it is proposed that FcaPV-3 is classified in the genus Taupapillomavirus and is the first non-canine PV in this genus.

  19. Genomic characterisation of Felis catus papillomavirus 4, a novel papillomavirus detected in the oral cavity of a domestic cat.

    Science.gov (United States)

    Dunowska, Magdalena; Munday, John S; Laurie, Rebecca E; Hills, Simon F K

    2014-02-01

    Three papillomaviruses (PVs) from the domestic cat have been fully sequenced so far including Felis domesticus PV-1 (FdPV-1), FdPV-2, and a recently described Felis catus PV-3 (FcaPV-4). In the current article, we describe the full genomic sequence of a fourth PV from the domestic cat. This PV was amplified from the oral cavity of a cat with severe gingivitis. However, the aetiological involvement of FcaPV-4 in development of lesions observed in this cat remains uncertain. The complete genome of the novel virus comprised 7,616 bp and was predicted to encode five early (E1, E2, E4, E6 and E7) and two late (L1 and L2) genes, with the organisation typical for PVs. The L1 showed 65.1 % nucleotide sequence identity to L1 of FcaPV-3 and approximately 60 % identity to L1 of canine tau-papillomaviruses CPV-2 and CPV-7. The novel virus clustered with FcaPV-3, CPV-2 and CPV-7 on a phylogenetic tree constructed from a concatenated alignment of 3,013 bp from E1, E2, L1 and L2. Based on the genomic and phylogenetic data, we propose that the novel virus is classified as a distinct species within the same genus as FcaPV-3. We also propose that both viruses are classified within the genus Taupapillomavirus, although this classification may need to be re-visited after more tau-PV genomes become available.

  20. A gregarine from the gut of cat flea, Ctenocephalides felis (Bouché) (Siphonaptera: Pulicidae) in Taiwan: dynamic of infection patterns.

    Science.gov (United States)

    Alarcón, Mauricio E; Huang, Chin-Gi; Dubey, Anil Kumar; Benítez, Hugo A

    2013-02-18

    An understanding on host-parasite interaction is essential for control of disease causing organisms in domestic animals. The cat flea, Ctenocephalides felis (Bouché) is the predominant flea infesting dogs and cats in Taiwan. It was collected from 933 dogs and 197 cats from Taiwan. A total of 5878 C. felis adults were recovered; 14.6% fleas were observed to harbor Steinina ctenocephali. Female fleas were more susceptible to gregarine infection than males. Further, fleas were more likely to be infected with the gregarine at high temperatures, particularly during March-July with high parasite prevalence and intensity. Fleas harboring gregarines infection were higher in dogs than cats. Our study may help in development and application of appropriate flea control measures in Taiwan.

  1. Factors associated with upper respiratory tract disease caused by feline herpesvirus, feline calicivirus, Chlamydophila felis and Bordetella bronchiseptica in cats: experience from 218 European catteries

    OpenAIRE

    Helps, C. R.; Lait, P.; Damhuis, A.; Björnehammar, U.; Bolta, D.; Brovida, C.; Chabanne, L.; Egberink, H; Ferrand, G.; Fontbonne, A.; Pennisi, M G; Gruffydd-Jones, T.; Gunn-Moore, D.; Hartmann, K.; Lutz, H

    2005-01-01

    A full history of the management practices and the prevalence of upper respiratory tract disease (URTD) at 218 rescue shelters, breeding establishments and private households with five or more cats was recorded. Oropharyngeal and conjunctival swabs and blood samples were taken from 1748 cats. The prevalences of feline herpesvirus (FHV), feline calicivirus (FCV), Chlamydophila felis and Bordetella bronchiseptica were determined by PCR on swab samples. An ELISA was applied to determine the prev...

  2. Efficacy of emodepside/toltrazuril suspension (Procox® oral suspension for dogs) against mixed experimental Isospora felis/Isospora rivolta infection in cats.

    Science.gov (United States)

    Petry, Gabriele; Kruedewagen, Eva; Kampkoetter, Andreas; Krieger, Klemens

    2011-08-01

    The coccidia Isospora felis and Isospora rivolta are intestinal parasites occurring worldwide in domestic cats. In young cats, they can be detected with higher prevalence.The effects of toltrazuril in the new combination product Procox(®) oral suspension for dogs containing 0.1 % emodepside and 2 % toltrazuril (0.9 mg emodepside + 18 mg toltrazuril per ml) were studied in eighteen kittens experimentally infected each with a total of 1 x 10(5) oocysts of a mixture of Isospora felis and Isospora rivolta. In the infectious material, the quantitative relation of I. felis and I. rivolta was about 1:5. Following a three-days period after infection, two groups of 6 kittens were treated during the prepatent period with either a single dose of 0.45 mg emodepside + 9 mg toltrazuril/kg body weight or 0.9 mg emodepside + 18 mg toltrazuril/kg body weight. A group of six kittens without any treatment served as a control. On day 5 post infection, the untreated kittens started the excretion of oocysts. Treatment with both toltrazuril doses significantly reduced oocyst excretion. Following the single higher dose, the reduction of oocysts of both Isospora spp. was more pronounced (96.7 % to 100 %) in comparison to the lower dose (57.2 % to 100 %). The Procox(®) application was well tolerated and no adverse events were seen with any of the applied dosages.When administered to kittens and as a single treatment during the prepatent period, Procox(®) is suitable to control the number of oocysts excreted in the faeces in case of an Isospora felis and Isospora rivolta infection.

  3. Antifungal activity of extracts from Atacama Desert fungi againstParacoccidioides brasiliensis and identification ofAspergillus felis as a promising source of natural bioactive compounds

    Directory of Open Access Journals (Sweden)

    Graziele Mendes

    2016-03-01

    Full Text Available Fungi of the genus Paracoccidioides are responsible for paracoccidioidomycosis. The occurrence of drug toxicity and relapse in this disease justify the development of new antifungal agents. Compounds extracted from fungal extract have showing antifungal activity. Extracts of 78 fungi isolated from rocks of the Atacama Desert were tested in a microdilution assay against Paracoccidioides brasiliensis Pb18. Approximately 18% (5 of the extracts showed minimum inhibitory concentration (MIC values≤ 125.0 µg/mL. Among these, extract from the fungus UFMGCB 8030 demonstrated the best results, with an MIC of 15.6 µg/mL. This isolate was identified as Aspergillus felis (by macro and micromorphologies, and internal transcribed spacer, β-tubulin, and ribosomal polymerase II gene analyses and was grown in five different culture media and extracted with various solvents to optimise its antifungal activity. Potato dextrose agar culture and dichloromethane extraction resulted in an MIC of 1.9 µg/mL against P. brasiliensis and did not show cytotoxicity at the concentrations tested in normal mammalian cell (Vero. This extract was subjected to bioassay-guided fractionation using analytical C18RP-high-performance liquid chromatography (HPLC and an antifungal assay using P. brasiliensis. Analysis of the active fractions by HPLC-high resolution mass spectrometry allowed us to identify the antifungal agents present in the A. felis extracts cytochalasins. These results reveal the potential of A. felis as a producer of bioactive compounds with antifungal activity.

  4. Rickettsia typhi IN RODENTS AND R. felis IN FLEAS IN YUCATÁN AS A POSSIBLE CAUSAL AGENT OF UNDEFINED FEBRILE CASES

    Directory of Open Access Journals (Sweden)

    Gaspar PENICHE-LARA

    2015-04-01

    Full Text Available Rickettsia typhi is the causal agent of murine typhus; a worldwide zoonotic and vector-borne infectious disease, commonly associated with the presence of domestic and wild rodents. Human cases of murine typhus in the state of Yucatán are frequent. However, there is no evidence of the presence of Rickettsia typhi in mammals or vectors in Yucatán. The presence of Rickettsia in rodents and their ectoparasites was evaluated in a small municipality of Yucatán using the conventional polymerase chain reaction technique and sequencing. The study only identified the presence of Rickettsia typhi in blood samples obtained from Rattus rattus and it reported, for the first time, the presence of R. felis in the flea Polygenis odiosus collected from Ototylomys phyllotis rodent. Additionally, Rickettsia felis was detected in the ectoparasite Ctenocephalides felis fleas parasitizing the wild rodent Peromyscus yucatanicus. This study’s results contributed to a better knowledge of Rickettsia epidemiology in Yucatán.

  5. The karyotype of Cathorops sp, a marine catfish from Brazil

    Directory of Open Access Journals (Sweden)

    Vicente Gomes

    1992-01-01

    Full Text Available Cathorops sp has the diploid number of 54 chromosomes. The karyotype comprises lm + sm, 6m, 6sm and 14st pairs. This result is compared with those of other species of Ariidae, mainly Ariopsis felis and Arius dussumieri, found in literature.Foram realizados estudos cromossômicos em 25 espécimens de Cathorops sp pelo método de air-drying. O número modal diplóide encontrado foi de 54 cromossomos sendo 1 par m + sm, 6 pares m, 6 pares sm e 14 pares st. Os resultados são comparados com dados da literatura referentes a cromossomos de outros ariídeos, principalmente de Ariopsis felis e Arius dussumieri.

  6. Comparative speed of efficacy against Ctenocephalides felis of two oral treatments for dogs containing either afoxolaner or fluralaner.

    Science.gov (United States)

    Beugnet, Frederic; Liebenberg, Julian; Halos, Lenaïg

    2015-01-30

    A study was designed to compare the efficacy of NexGard(®) and Bravecto™, 2 recently introduced oral ectoparasiticides containing isoxazolines, against fleas (Ctenocephalides felis) on dogs. Twenty-four healthy dogs, weighing 9.2 kg to 28.6 kg, were included in this parallel group design, randomized, and controlled efficacy study. On Day -1, the 24 dogs were allocated to 3 study groups: untreated control; Nexgard(®) treated and Bravecto™ treated. The treatments were administered on Days 0, 28 and 56 for Nexgard(®) (labelled for monthly administration), and once on Day 0 for Bravecto™ (labelled for a 12 week use). Flea infestations were performed weekly with 100 adult unfed C. felis on each dog from Days 42 to 84. Fleas were counted and re-applied at 6 and 12 h post-infestation and removed and counted 24 h post-infestation. The arithmetic mean flea count for the untreated group ranged from 62.9 to 77.6 at 24 h post-infestation, indicating vigorous flea challenges on all assessment days. Both the Nexgard(®) and Bravecto™ treated groups had statistically significantly (p<0.05) less fleas compared to the untreated group on all assessment time points and days. Significantly fewer fleas were recorded for NexGard(®) treated dogs compared to Bravecto™ treated dogs at 6 h post-infestation on Day 56, 63, 70, 77 and 84 and at 12 h post-infestation on Days 70 and 84. No statistically significant (p<0.05) differences were recorded between the treated groups at 24 h post-infestation. Efficacies recorded 6 h post-infestation for Nexgard(®) ranged from 62.8% (Day 49) to 97.3% (Day 56), and efficacies ranged from 94.1% (Day 49) to 100% (Days 42, 56, 70 and 84) at 12 h post-infestation. Efficacies recorded for Bravecto™ ranged from 45.1% (Day 84) to 97.8% (Day 42) at 6 h post-infestation, and from 64.7% (Day 84) to 100% (Days 42 and 56) at 12 h post-infestation. Efficacies observed at 24 h were 100% for both products during the study except 99.6% on Day 84 for

  7. Influence de la température sur le développement de la puce africaine du chat Ctenocephalides felis strongylus (Jordan, 1925 (Siphonaptera : Pulicidae

    Directory of Open Access Journals (Sweden)

    Yao K.P.

    2010-06-01

    Full Text Available Ctenocephalides felis (Bouché, 1835 communément appelée “puce du chat” présente deux sous-espèces reconnues : Ctenocephalides felis strongylus (Jordan, 1925 inféodée au continent africain et Ctenocephalides felis felis (Bouché, 1835 présente dans les zones à climat tempéré (Afrique du Nord, Europe et Amérique (Ménier et Beaucournu, 1999. En Afrique subsaharienne, la principale puce retrouvée chez les animaux de compagnie et chez certains animaux d’élevage (ovins, caprins et bovins appartient à la sous-espèce C. f. strongylus. Quelques paramètres bio-écologiques de C. f. strongylus ont été étudiés dans différentes conditions d’élevage. Les résultats ont été comparés à ceux de C. f. felis actuellement disponibles. À 75% ± 5 d’humidité relative, le cycle de développement de C. f. strongylus dure 20-21 jours à 27 °C et de 16 à 17 jours à 29 °C. Ainsi, la sousespèce africaine de la puce du chat (C. f. strongylus se développe moins vite que C. f. felis à températures identiques. Cette différence pourrait s’expliquer par l’influence du climat de leurs aires de distribution respectives sur leur cycle de développement. À 75% ± 5 d’humidité relative, les adultes de C. f. strongylus ne peuvent survivre plus de 14 jours dans l’environnement à des températures comprises entre 27 et 29 °C, lorsqu’elles n’ont jamais pris de repas sanguin. Dans ces mêmes conditions, la durée de survie n’excède pas 16 jours à 19 °C. Mais lorsque C. f. strongylus a pris un premier repas de sang, elle a une durée de vie beaucoup plus courte lorsqu’elle est hors de son hôte. En effet, aucun individu n’est retrouvé vivant trois jours passé hors de la fourrure de son hôte à 29 °C, cinq jours à 27 °C et huit jours à 19 °C. Il en est de même pour C. f. felis. Ces données sur la bio-écologie de C. f. strongylus permettent de comprendre l’influence de la température sur son cycle de d

  8. Early-Onset Progressive Retinal Atrophy Associated with an IQCB1 Variant in African Black-Footed Cats (Felis nigripes)

    Science.gov (United States)

    Oh, Annie; Pearce, Jacqueline W.; Gandolfi, Barbara; Creighton, Erica K.; Suedmeyer, William K.; Selig, Michael; Bosiack, Ann P.; Castaner, Leilani J.; Whiting, Rebecca E. H.; Belknap, Ellen B.; Lyons, Leslie A.; Aderdein, Danielle; Alves, Paulo C.; Barsh, Gregory S.; Beale, Holly C.; Boyko, Adam R.; Castelhano, Marta G.; Chan, Patricia; Ellinwood, N. Matthew; Garrick, Dorian J.; Helps, Christopher R.; Kaelin, Christopher B.; Leeb, Tosso; Lohi, Hannes; Longeri, Maria; Malik, Richard; Montague, Michael J.; Munday, John S.; Murphy, William J.; Pedersen, Niels C.; Rothschild, Max F.; Swanson, William F.; Terio, Karen A.; Todhunter, Rory J.; Warren, Wesley C.

    2017-01-01

    African black-footed cats (Felis nigripes) are endangered wild felids. One male and full-sibling female African black-footed cat developed vision deficits and mydriasis as early as 3 months of age. The diagnosis of early-onset progressive retinal atrophy (PRA) was supported by reduced direct and consensual pupillary light reflexes, phenotypic presence of retinal degeneration, and a non-recordable electroretinogram with negligible amplitudes in both eyes. Whole genome sequencing, conducted on two unaffected parents and one affected offspring was compared to a variant database from 51 domestic cats and a Pallas cat, revealed 50 candidate variants that segregated concordantly with the PRA phenotype. Testing in additional affected cats confirmed that cats homozygous for a 2 base pair (bp) deletion within IQ calmodulin-binding motif-containing protein-1 (IQCB1), the gene that encodes for nephrocystin-5 (NPHP5), had vision loss. The variant segregated concordantly in other related individuals within the pedigree supporting the identification of a recessively inherited early-onset feline PRA. Analysis of the black-footed cat studbook suggests additional captive cats are at risk. Genetic testing for IQCB1 and avoidance of matings between carriers should be added to the species survival plan for captive management. PMID:28322220

  9. On-host viability and fecundity of Ctenocephalides felis (Siphonaptera: Pulicidae), using a novel chambered flea technique.

    Science.gov (United States)

    Thomas, R E; Wallenfels, L; Popiel, I

    1996-03-01

    The on-host viability and fecundity of cat fleas, Ctenocephalides felis (Bouché), confined within a novel chambering system are described. Using this system, all fleas and flea eggs are recovered from chambers after fleas have fed on cats. Thus, accurate calculations of both adult flea survival and female flea fecundity can be made. The technique provides a microenvironment in which adult fleas exhibit > 90% survival over 14 d. Female fleas lay an average of 9.5 eggs per day on the 2nd d of feeding, 22.1 eggs per day between days 3 and 7, and 19.6 eggs per day between days 3 and 14. These numbers are similar to values previously reported for studies in which fleas were not confined. The technique permits accurate, multiple sampling of experimental flea populations during a study, and does not require the use of pesticides or extensive combing to collect surviving fleas at the end of a study. Moreover, the technique does not require that cats be caged or prevented from grooming. Collecting data from fleas confined in chambers is much less time consuming and labor intensive than studies with free-roaming fleas.

  10. Techniques used in the study of African wildcat, Felis silvestris cafra, in the Kgalagadi Transfrontier Park (South Africa/Botswana

    Directory of Open Access Journals (Sweden)

    Marna Herbst

    2010-03-01

    Full Text Available The techniques used for the capture, marking and habituation of African wildcats (Felis silvestris cafra in the Kalahari are described and evaluated in this paper. African wildcats were captured, with either baited cage traps or chemical immobilisation through darting. Darting proved to be a more efficient and less stressful way of capturing cats. Very high frequency (VHF radio collars fitted with activity monitors were especially effective in the open habitat of the Kalahari for locating and maintaining contact with cats; they also aided in determining if the cats were active or resting in dense vegetation. The habituation of individual cats to a 4×4 vehicle proved to be time consuming, but it provided a unique opportunity to investigate the feeding ecology and spatial organisation of cats through direct visual observations.Conservation implications: In describing and comparing the various methods of capture, handling and release of the African wildcats that we followed during our study in the southern Kalahari, we recommend the most efficient, least stressful method for researchers to follow – both in relation to time and energy, as well as in terms of the impact on the animals being studied.

  11. Relationship between the Presence of Bartonella Species and Bacterial Loads in Cats and Cat Fleas (Ctenocephalides felis) under Natural Conditions.

    Science.gov (United States)

    Gutiérrez, Ricardo; Nachum-Biala, Yaarit; Harrus, Shimon

    2015-08-15

    Cats are considered the main reservoir of three zoonotic Bartonella species: Bartonella henselae, Bartonella clarridgeiae, and Bartonella koehlerae. Cat fleas (Ctenocephalides felis) have been experimentally demonstrated to be a competent vector of B. henselae and have been proposed as the potential vector of the two other Bartonella species. Previous studies have reported a lack of association between the Bartonella species infection status (infected or uninfected) and/or bacteremia levels of cats and the infection status of the fleas they host. Nevertheless, to date, no study has compared the quantitative distributions of these bacteria in both cats and their fleas under natural conditions. Thus, the present study explored these relationships by identifying and quantifying the different Bartonella species in both cats and their fleas. Therefore, EDTA-blood samples and fleas collected from stray cats were screened for Bartonella bacteria. Bacterial loads were quantified by high-resolution melt real-time quantitative PCR assays. The results indicated a moderate correlation between the Bartonella bacterial loads in the cats and their fleas when both were infected with the same Bartonella species. Moreover, a positive effect of the host infection status on the Bartonella bacterial loads of the fleas was observed. Conversely, the cat bacterial loads were not affected by the infection status of their fleas. Our results suggest that the Bartonella bacterial loads of fleas are positively affected by the presence of the bacteria in their feline host, probably by multiple acquisitions/accumulation and/or multiplication events.

  12. Control of immature stages of the flea Ctenocephalides felis(Bouché in carpets exposed to cats treated with imidacloprid

    Directory of Open Access Journals (Sweden)

    L.J. Fourie

    2000-07-01

    Full Text Available Fleas cause allergic dermatitis in cats and dogs and therefore warrant control. It has been demonstrated previously that there is marked inhibition of the development of the immature stages of the cat flea Ctenocephalides felis on fleece blankets exposed to cats treated with imidacloprid. This study reports on the efficacy of imidacloprid in suppressing adult flea emergence in carpet exposed to treated cats. Circular discs of carpet pre-seeded with flea eggs and larvae were exposed to 6 untreated control and 6 topically treated (imidacloprid 10 % m/v cats 1 to 2 days after treatment and subsequently fortnightly for 6 weeks. Exposure times on alternate days were either 1 or 6 hours. Adult flea yield from carpets was determined 35 days after exposure. Differences between flea yield on control carpets and those exposed for 1 hour were significant only for days +1 and +14. For the 6-hour exposure, differences were significant at all times except on Day +43. The ability of imidacloprid to suppress the yield of adult fleas on carpets (6-hour exposure steadily declined from 82 % (Day +2 to 12 %(Day +43. For the 1-hour exposure it varied inconsistently between 0 and 83 % over the 6-week study period.

  13. Hybridization versus conservation: are domestic cats threatening the genetic integrity of wildcats (Felis silvestris silvestris) in Iberian Peninsula?

    Science.gov (United States)

    Oliveira, Rita; Godinho, Raquel; Randi, Ettore; Alves, Paulo C

    2008-09-12

    Cross-breeding between wild and free-ranging domestic species is one of the main conservation problems for some threatened species. The situation of wildcats (Felis silvestris silvestris) in Europe is a good example of this critical phenomenon. Extensive hybridization was described in Hungary and Scotland, contrasting with occasional interbreeding in Italy and Germany. First analyses in Portugal revealed a clear genetic differentiation between wild and domestic cats; however, four hybrids were detected. Here, we extended the approach to Iberian Peninsula using multivariate and Bayesian analyses of multilocus genotypes for 44 Portuguese wildcats, 31 Spanish wildcats and 109 domestic cats. Globally, wild and domestic cats were significantly differentiated (FST=0.20, ppower of admixture analyses was assessed by simulating hybrid genotypes, which revealed that used microsatellites were able to detect 100, 91 and 85% of first-generation hybrids, second-generation genotypes and backcrosses, respectively. These findings suggest that the true proportion of admixture can be higher than the value estimated in this study and that the improvement of genetic tools for hybrids detection is crucial for wildcat conservation.

  14. A Pre-Restoration Fishery Resource Utilization Assessment of an Estuarine Borrow Pit in Mobile Bay, Alabama

    Science.gov (United States)

    2013-06-01

    felis Hardhead Catfish - - 7 7 14 - - - 0 0 - - - 0 0 Cynoscion arenarius White Trout 2 4 - 6 12 - - - 0 0 - - - 0 0 Bairdiella chrysoura...Bagre marinus Gafftopsail Catfish - - 1 1 2 - - - 0 0 - - - 0 0 Brevoortia patronus Gulf Menhaden - - - 1 2 - - - 0 0 - - - 0 0 Larimus...arenarius White Trout 2 1 - 3 6 - - - 0 0 - 1 3 4 8 Bairdiella chrysoura Silver Perch 1 1 4 6 12 - - - 0 0 - - - 0 0 Arius felis Hardhead Catfish

  15. Multidimensional effects of biologically synthesized silver nanoparticles in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma A549 cells

    Science.gov (United States)

    Gurunathan, Sangiliyandi; Jeong, Jae-Kyo; Han, Jae Woong; Zhang, Xi-Feng; Park, Jung Hyun; Kim, Jin-Hoi

    2015-02-01

    Silver nanoparticles (AgNPs) are prominent group of nanomaterials and are recognized for their diverse applications in various health sectors. This study aimed to synthesize the AgNPs using the leaf extract of Artemisia princeps as a bio-reductant. Furthermore, we evaluated the multidimensional effect of the biologically synthesized AgNPs in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma (A549) cells. UV-visible (UV-vis) spectroscopy confirmed the synthesis of AgNPs. X-ray diffraction (XRD) indicated that the AgNPs are specifically indexed to a crystal structure. The results from Fourier transform infrared spectroscopy (FTIR) indicate that biomolecules are involved in the synthesis and stabilization of AgNPs. Dynamic light scattering (DLS) studies showed the average size distribution of the particle between 10 and 40 nm, and transmission electron microscopy (TEM) confirmed that the AgNPs were significantly well separated and spherical with an average size of 20 nm. AgNPs caused dose-dependent decrease in cell viability and biofilm formation and increase in reactive oxygen species (ROS) generation and DNA fragmentation in H. pylori and H. felis. Furthermore, AgNPs induced mitochondrial-mediated apoptosis in A549 cells; conversely, AgNPs had no significant effects on L132 cells. The results from this study suggest that AgNPs could cause cell-specific apoptosis in mammalian cells. Our findings demonstrate that this environmentally friendly method for the synthesis of AgNPs and that the prepared AgNPs have multidimensional effects such as anti-bacterial and anti-biofilm activity against H. pylori and H. felis and also cytotoxic effects against human cancer cells. This report describes comprehensively the effects of AgNPs on bacteria and mammalian cells. We believe that biologically synthesized AgNPs will open a new avenue towards various biotechnological and biomedical applications in the near future.

  16. Efecto del agonista de GnRH acetato de deslorelina en felinos domésticos (Felis catus) prepúberes

    OpenAIRE

    Carranza, Ana

    2015-01-01

    Debido a la marcada prolificidad propia de la especie, la sobrepoblación de felinos domésticos (Felis catus) representa un problema mundial. Bajo el objetivo general de brindar un aportate al control de la reproducción indeseada felina, en este Trabajo de Tesis: se evaluó el patrón posnatal de los esteroides sexuales fecales, se estudió la eficacia (postergación de la pubertad e infertilidad) y la seguridad clínica de un agonista de larga duración (acetato de deslorelina) y un antagonista (ac...

  17. Ecology driving genetic variation: a comparative phylogeography of jungle cat (Felis chaus and leopard cat (Prionailurus bengalensis in India.

    Directory of Open Access Journals (Sweden)

    Shomita Mukherjee

    Full Text Available BACKGROUND: Comparative phylogeography links historical population processes to current/ecological processes through congruent/incongruent patterns of genetic variation among species/lineages. Despite high biodiversity, India lacks a phylogeographic paradigm due to limited comparative studies. We compared the phylogenetic patterns of Indian populations of jungle cat (Felis chaus and leopard cat (Prionailurus bengalensis. Given similarities in their distribution within India, evolutionary histories, body size and habits, congruent patterns of genetic variation were expected. METHODOLOGY/PRINCIPAL FINDINGS: We collected scats from various biogeographic zones in India and analyzed mtDNA from 55 jungle cats (460 bp NADH5, 141 bp cytochrome b and 40 leopard cats (362 bp NADH5, 202 bp cytochrome b. Jungle cats revealed high genetic variation, relatively low population structure and demographic expansion around the mid-Pleistocene. In contrast, leopard cats revealed lower genetic variation and high population structure with a F(ST of 0.86 between North and South Indian populations. Niche-model analyses using two approaches (BIOCLIM and MaxEnt support absence of leopard cats from Central India, indicating a climate associated barrier. We hypothesize that high summer temperatures limit leopard cat distribution and that a rise in temperature in the peninsular region of India during the LGM caused the split in leopard cat population in India. CONCLUSIONS/SIGNIFICANCE: Our results indicate that ecological variables describing a species range can predict genetic patterns. Our study has also resolved the confusion over the distribution of the leopard cat in India. The reciprocally monophyletic island population in the South mandates conservation attention.

  18. Somatotopic organization of the facial lobe of the sea catfish Arius felis studied by transganglionic transport of horseradish peroxidase.

    Science.gov (United States)

    Kiyohara, S; Caprio, J

    1996-04-22

    To reveal the somatotopical organization of the facial lobe (FL), a primary medullary gustatory nucleus in the sea catfish Arius felis, the central projections of the peripheral rami of the facial nerve innervating taste buds located across the entire body surface and rostral oral regions were traced by means of horseradish peroxidase neurohistochemistry. The maxillary barbel, lateral mandibular barbel, medial mandibular barbel, and trunk-tail branches project to four different longitudinal columns (i.e., lobules) extending rostrocaudally in the FL. The trunk-tail lobule, which is located dorsolateral to the barbel lobules, lies in the anterior two-thirds of the FL. The tail is represented in a more rostral portion of the trunk-tail lobule than the trunk, indicating that the rostrocaudal trunk axis is represented in the trunk-tail lobule in a posteroanterior axis. The pectoral fin branch ends in an intermediate region of the FL, whereas the hyomandibular, ophthalmic, lower lip, upper lip, and palatine branches terminate in discrete regions of the caudal one-third of the FL. These results reveal a sharply defined somatotopical organization of the FL of Arius and support the hypothesis that the number and lengths of the barbel lobules within the FL of catfishes are directly related to the number and relative lengths of the barbels. An additional subcolumn, the intermediate nucleus of the FL (NIF), which develops in the medioventral region of the caudal two-thirds of the FL, receives projections in a diffuse somatotopical fashion from the barbels, lower lip, and palatine branches. Trigeminal fibers of the barbel and lower lip branches project in a somatotopic fashion to the FL. The present findings suggest that the FL of Arius is highly organized somatotopically to detect, by tropotaxis, precise spatial information concerning taste and tactile stimuli in the environment.

  19. Vocal correlates of sender-identity and arousal in the isolation calls of domestic kitten (Felis silvestris catus

    Directory of Open Access Journals (Sweden)

    Scheumann Marina

    2012-12-01

    Full Text Available Abstract Introduction Human speech does not only communicate linguistic information but also paralinguistic features, e.g. information about the identity and the arousal state of the sender. Comparable morphological and physiological constraints on vocal production in mammals suggest the existence of commonalities encoding sender-identity and the arousal state of a sender across mammals. To explore this hypothesis and to investigate whether specific acoustic parameters encode for sender-identity while others encode for arousal, we studied infants of the domestic cat (Felis silvestris catus. Kittens are an excellent model for analysing vocal correlates of sender-identity and arousal. They strongly depend on the care of their mother. Thus, the acoustical conveyance of sender-identity and arousal may be important for their survival. Results We recorded calls of 18 kittens in an experimentally-induced separation paradigm, where kittens were spatially separated from their mother and siblings. In the Low arousal condition, infants were just separated without any manipulation. In the High arousal condition infants were handled by the experimenter. Multi-parametric sound analyses revealed that kitten isolation calls are individually distinct and differ between the Low and High arousal conditions. Our results suggested that source- and filter-related parameters are important for encoding sender-identity, whereas time-, source- and tonality-related parameters are important for encoding arousal. Conclusion Comparable findings in other mammalian lineages provide evidence for commonalities in non-verbal cues encoding sender-identity and arousal across mammals comparable to paralinguistic cues in humans. This favours the establishment of general concepts for voice recognition and emotions in humans and animals.

  20. Avaliação dos estoques pesqueiros de quatro espécies de grandes bagres (Siluriformes, Pimelodidae na bacia do rio Cuiabá, Pantanal norte, Brasil, utilizando alguns Pontos de Referência Biológicos Fisheries stock assessment of four large catfish (Siluriformes, Pimelodidae in the Cuiaba River basin, north Pantanal, Brazil, using some Biological Reference Points

    Directory of Open Access Journals (Sweden)

    Lúcia A. F. Mateus

    2007-03-01

    Full Text Available Os parâmetros de crescimento e mortalidade estimados a partir da estrutura em comprimento foram utilizados para avaliar o estado de explotação dos estoques de quatro espécies de grandes bagres da bacia do rio Cuiabá, Pantanal norte. As espécies estudadas foram o barbado, o cachara, o jaú e o pintado. As avaliações foram realizadas por meio do modelo de rendimento por recruta, considerando os Pontos de Referência Biológicos Fmax, F0,1, F = M e F BDR. As análises indicam que os estoques do pintado e do jaú estão bem conservados. Os estoques do cachara e, menos fortemente, o do barbado estão em iminente ameaça de sobrepesca na bacia do rio Cuiabá.The growth and mortality parameters estimated from length structure were used to evaluate the state of explotation of the stocks of four large catfish species from Cuiabá River basin, north Pantanal. The species studied were the Flatwhiskered catfish, the Jaú, the Barred sorubim, and the Spotted sorubim. The assessment were conducted with the yield per recruit model, and the Biological Reference Points Fmax, F0.1, F = M, and F BDR. The analysis indicates that the stocks of the Spotted sorubim, and of the Jaú are not overexploited. However, the stocks of the Barred sorubim, and, in lower degree, of the Flatwhiskered catfish of the Cuiabá River basin are in imminent threat of overfishing.

  1. Seroprevalence of Rickettsia bellii and Rickettsia felis in dogs, São José dos Pinhais, State of Paraná, Brazil Soroprevalência de Rickettsia bellii e Rickettsia felis em cães, São José dos Pinhais, Paraná, Brasil

    Directory of Open Access Journals (Sweden)

    Fernanda Silva Fortes

    2010-12-01

    Full Text Available Brazilian spotted fever (BSF is a vector-borne zoonosis caused by Rickettsia rickettsii bacteria. Dogs can be host sentinels for this bacterium. The aim of the study was to determine the presence of antibodies against Rickettsia spp. in dogs from the city of São José dos Pinhais, State of Paraná, Southern Brazil, where a human case of BSF was first reported in the state. Between February 2006 and July 2007, serum samples from 364 dogs were collected and tested at 1:64 dilutions by indirect immunofluorescence assay (IFA against R. rickettsii and R. parkeri. All sera that reacted at least to one of Rickettsia species were tested against the six main Rickettsia species identified in Brazil: R. rickettsii, R. parkeri, R. bellii, R. rhipicephali, R. amblyommii and R. felis. Sixteen samples (4.4% reacted to at least one Rickettsia species. Among positive animals, two dogs (15.5% showed suggestive titers for R. bellii exposure. One sample had a homologous reaction to R. felis, a confirmed human pathogen. Although Rickettsia spp. circulation in dogs in the area studied may be considered at low prevalence, suggesting low risk of human infection, the present data demonstrate for the first time the exposure of dogs to R. bellii and R. felis in Southern Brazil.A febre maculosa brasileira (FMB é uma zoonose veiculada por carrapatos e causada pela bactéria Rickettsia rickettsii, podendo os cães ser hospedeiros sentinelas para essa bactéria. O objetivo do estudo foi determinar a presença de anticorpos contra Rickettsia spp. em cães de São José dos Pinhais, estado do Paraná, Sul do Brasil. Entre fevereiro de 2006 e julho de 2007, amostras séricas de 364 cães foram coletadas e testadas na diluição de 1:64 por Reação de Imunofluorescência Indireta (RIFI contra R. rickettsii e R. parkeri. Todos os soros reagentes para pelo menos uma espécie de Rickettsia foram testados contra as seis principais espécies de Rickettsia identificadas no Brasil: R

  2. Evaluation of the curative and preventive efficacy of a single oral administration of afoxolaner against cat flea Ctenocephalides felis infestations on dogs.

    Science.gov (United States)

    Hunter, James S; Dumont, Pascal; Chester, Theodore S; Young, David R; Fourie, Josephus J; Larsen, Diane L

    2014-04-02

    The efficacy of orally administered afoxolaner for treatment and prevention of repeated infestations with adult Ctenocephalides felis on dogs was evaluated in two studies after administration of a beef-flavored soft chew. In each study, 32 dogs were divided randomly into four equal groups. Dogs in Groups 1 and 3 were not treated and served as controls. Dogs in Groups 2 and 4 were treated on Day 0 with a combination of chewable tablets to be as close as possible to the minimum therapeutic dose of 2.5mg/kg. All animals were infested experimentally with unfed C. felis (100 ± 5) on Days -1, 7, 14, 21, 28 and 35. Flea killing efficacy was evaluated in both studies while, efficacy against flea egg production was assessed in Study 1. Live fleas were counted at 12 (Groups 1 and 2) and 24h (Groups 3 and 4), after treatment or after weekly infestations. In Study 1, flea eggs were collected and counted at either 12 or 24h after each flea infestation on Days 7, 14, 21, 28 and 35. The results of both studies demonstrate the long lasting and rapid efficacy of afoxolaner against C. felis, when administered as a single oral dose to dogs. For flea counts conducted 24h after treatment or infestation, efficacy was 100% for all time points up to Day 36 in both studies, except for one time point (99.9% on Day 22) for Study 2. For flea counts performed 12h after treatment or infestation, efficacy was ≥ 95.2% until Day 21 in both studies. Efficacy at 12h was ≥ 93.0% on Day 35 in Study 1 and ≥ 89.7% on Day 35 in Study 2. The treated groups had significantly fewer fleas than untreated control dogs in both studies for all flea counts (p=0.003 Study 1, p=0.0006 Study 2). In Study 1, for all egg counts performed at or beyond Day 7, efficacy in egg reduction was >99% for all time points between Days 7 and 35.

  3. Detection of Hepatozoon felis in Ticks Collected from Free-Ranging Amur Tigers ( Panthera tigris altaica), Russian Far East, 2002-12.

    Science.gov (United States)

    Thomas, Lindsay H; Seryodkin, Ivan V; Goodrich, John M; Miquelle, Dale G; Birtles, Richard J; Lewis, John C M

    2016-07-01

    We collected 69 ticks from nine, free-ranging Amur tigers ( Panthera tigris altaica) between 2002 and 2011 and investigated them for tick-borne pathogens. DNA was extracted using alkaline digestion and PCR was performed to detect apicomplexan organisms. Partial 18S rDNA amplification products were obtained from 14 ticks from four tigers, of which 13 yielded unambiguous nucleotide sequence data. Comparative sequence analysis revealed all 13 partial 18S rDNA sequences were most similar to those belonging to strains of Hepatozoon felis (>564/572 base-pair identity, >99% sequence similarity). Although this tick-borne protozoon pathogen has been detected in wild felids from many parts of the world, this is the first record from the Russian Far East.

  4. Reprodução do bagre amarelo, Cathorops spixii (Agassiz (Siluriformes, Ariidae, da Baía de Pinheiros, região estuarina do litoral do Paraná, Brasil Reproduction of the Madamango sea catfish, Cathorops spixii (Agassiz (Siluriformes, Ariidae, of the Pinheiros Bay, estuarine coastal area of Paraná, Brazil

    Directory of Open Access Journals (Sweden)

    Luís F. Fávaro

    2005-12-01

    Full Text Available O presente estudo investigou a biologia reprodutiva do bagre amarelo Cathorops spixii (Agassiz, 1829 na Baía de Pinheiros, litoral do Paraná, Brasil. Os peixes foram coletados mensalmente no período de junho de 2003 a maio de 2004, com rede de arrasto de fundo. De cada exemplar foram tomados os dados de comprimento total, peso total, sexo e peso da gônada. As seis fases de desenvolvimento ovocitário (ovogênese e os cinco estádios de desenvolvimento ovariano (escala de maturidade foram determinadas por análises microscópicas dos ovários. Através dos valores individuais e media mensais do Índice gônado-somático (IGS tratado para sexos separados, da freqüência relativa dos estádios de desenvolvimento ovariano e das análises histológicas durante o período de estudo foi possível determinar que o período reprodutivo de C. spixii dá-se entre os meses de setembro e novembro. Fêmeas apresentando ovários desovados ou em recuperação foram registradas nos meses de novembro a abril, com altos percentuais no período de dezembro a março. A desova foi caracterizada como sendo do tipo total.The present study investigated the reproductive biology of Cathorops spixii (Agassiz, 1829 in Pinheiros Bay, littoral of Paraná State, Brazil. Fishes were captured monthly from June 2003 to May 2004 by using bottom trawls. For each individual, total body length, total weight, sex, and gonadal weight were recorded. The oogenesis (six ovarian follicle phases of development and gonadal development stages (five stages were also defined based on the histological examination of the ovaries. It was defined that the reproductive period of C. spixii extends from September to November, based on the analyses of individual gonadosomatic index (GSI values distribution in relation to the sampling periods, on the monthly variation of the GSI mean values (Curve of Maturation, both for males and females, and on the monthly variation of the relative frequencies of

  5. Paragonimus y Paragonimiasis en el norte peruano. Proceso del desarrollo de Paragonimus peruvianus Miyazaki¡ Ibañez y Miranda¡ 1969 en Felis cati L. gato doméstico infectado experimentalmente.

    Directory of Open Access Journals (Sweden)

    Nicanor Ibáñez H.

    2014-06-01

    Full Text Available Se ha estudiado el proceso del desarrollo de Parogonimus peruvianus Miyazaki, Ibóñez y Miranda, 1969 en el gato doméstico, Felis cati L. infectado experimentalmente mediante la administración oral de metacercarias del parasito extraídas de ejemplares de Pseudothelphusa chilensis Milne Edwards, 1843 procedentes de áreas endémicas.

  6. Prevalence of Bartonella species DNA and antibodies in cats (Felis catus) submitted to a spay/neuter program in Rio de Janeiro, Brazil.

    Science.gov (United States)

    Crissiuma, Ana; Favacho, Alexsandra; Gershony, Liza; Mendes-de-Almeida, Flavya; Gomes, Raphael; Mares-Guia, Angélica; Rozental, Tatiana; Barreira, Jairo; Lemos, Elba; Labarthe, Norma

    2011-02-01

    The prevalence of Bartonella species DNA and antibodies for Bartonella henselae were studied in 40 clinically healthy cats (Felis catus, Linnaeus 1758) submitted to a spay/neuter program in Rio de Janeiro, Brazil. Additionally, the prevalence of Bartonella species DNA was investigated in the fleas found parasitizing the subject cats. For this purpose, blood samples were obtained from all cats, and DNA extraction was performed on the blood, and blood clotted samples, as well as on pools of fleas obtained from them. Antibodies for B henselae were detected on serum samples. Bartonella species DNA was detected in 17 cats, whereas serum reactivity for B henselae was found in 19. A total of 20 cats were flea-infested and nine of these 20 had Bartonella species DNA in their blood. In four of the 20 flea-infested cats, Bartonella species DNA was detected in the fleas obtained from those cats, but only one of these four cats had Bartonella species DNA in its blood.

  7. ASPECTOS COMPARATIVOS ENTRE AS TÉCNICAS DE ALIMENTAÇÃO ENTERAL: INTUBAÇÃO NASOESOFAGIANA E ESOFAGOSTOMIA EM FELINOS DOMÉSTICOS (Felis domestica

    Directory of Open Access Journals (Sweden)

    Renata Sousa Ramos

    2006-01-01

    Full Text Available Eight domestic felines (Felis domestica, males, adults, healthy, without a defined breed, with variable weights and ages, were randomly divided into two groups of four animals and submitted to the enteral feeding techniques: nasoesophageal intubation and esophagostomy, aiming to compare clinically and surgically both techniques. The average time required for the nasoesophageal intubation (1min 15sec was significantly smaller than for the esophagostomy (5min 25sec according to Fisher¿s test. In the nasoesophageal intubation technique, an easiness in the positioning of the feeding tube was observed when compared to the esophagostomy technique, being accomplished only with local topical anesthetics. All the animals submitted to the nasoesophageal intubation technique presented sneezes and uneasiness during the feeding supply, compared to those submitted to the esophagostomy technique. Conclusions were that the nasoesophageal intubation technique allows smaller surgical and, consequently, anesthetic time; it is less traumatic; the animals submitted to the esophagostomy technique have accepted the tube better; the techniques are easily accomplished and allow the nutritional maintenance of the animals.

  8. Food habits and temporal activity patterns of the Golden Jackal Canis aureus and the Jungle Cat Felis chaos in Pench Tiger Reserve, Madhya Pradesh

    Directory of Open Access Journals (Sweden)

    A. Majumder

    2011-11-01

    Full Text Available The food habits and temporal activity patterns of the Golden Jackal Canis aureus and the Jungle Cat Felis chaus were studied between January 2008 and June 2009 in Pench Tiger Reserve (PTR, Madhya Pradesh. A total of 50 jackal scats and 85 jungle cat scats were collected where-ever encountered in the study area. Information on activity pattern was obtained using camera traps. Fifty-two pair self-triggered analog cameras were deployed in each 2 x 2 km² across the study area (> 250 km² close to animal trails which were set to work on a continuous 24 hour period. Rodents contributed maximum in the diet of these two species (65% golden jackal scats and 56% jungle cat scats. Eight thousand five hundred and sixty camera-trap nights revealed 189 jungle cat captures and 49 golden jackal captures. The activity of golden jackal had a more homogeneous distribution in time. Present study showed that although some degree of overlap is observed between the two sympatric species, an overall difference in dietary composition and activity patterns enabled them to coexist in PTR.

  9. Current Status and Protection of Felis Lynx Population in the Northeast of China%东北地区猞猁种群现状及其保护

    Institute of Scientific and Technical Information of China (English)

    田家龙; 李贵臣; 孙红瑜

    2002-01-01

    猞猁(Felis Lynx)属食肉目猫科动物,在我国主要分布在东北、华北、青藏高原等地,近年来由于森林资源的大量开发,严重地破坏了生态平衡,特别是由于无限制地猎取野兔,使其食物链巨减,因此,各地猞猁种群明显下降.我们利用三阶抽样法,查清了东北地区猞猁种群的分布及数量情况.结果表明,东北地区猞猁分布区域只剩下大小兴安岭及长白山部分地区,总栖息面积仅有200 000km2,平均密度为0.013238只/kma,既每75km2有1只,数量不到3000只,种群处于极其濒危状态,应禁止任何理由的猎捕利用,严加保护并采取一切措施,使野生种群尽快恢复.

  10. Ectoparasitic species from Felis catus domesticus (Linnaeus, 1758 in João Pessoa city, Paraíba state, Brazil

    Directory of Open Access Journals (Sweden)

    Débora Rochelly Alves Ferreira

    2010-12-01

    Full Text Available The goal of this research was to analyze the ectoparasitic species from domestic cats in João Pessoa city, Paraíba state, Brazil. A total of 432 cats of various breeds and ages, of both genders, were included. All animals were submitted to physical and parasitological examination through skin inspection and an otoscopic exam was realized. Parasites were collected in vials containing 70% alcohol for subsequent mounting and identifi cation according to specific keys. The results showed that 62.7% (271/432 of animals were parasitized by one or more species. The ectoparasitic species observed were Ctenocephalides felis (27.3%, Lynxacarus radovskyi (26.2%, Otodectes cynotis (17.4%, Felicola subrostratus (9.7%, Notoedres cati (2.1% and Rhipicephalus sanguineus (1.6%. The results showed that ectoparasites are a common and important skin disease in cats, and variables such breed, age, and sex did not show any influence on parasitism.

  11. Complete nucleotide sequences of the domestic cat (Felis catus) mitochondrial genome and a transposed mtDNA tandem repeat (Numt) in the nuclear genome

    Energy Technology Data Exchange (ETDEWEB)

    Lopez, J.V.; Cevario, S.; O`Brien, S.J. [National Cancer Institute, Frederick, MD (United States)

    1996-04-15

    The complete 17,009-bp mitochondrial genome of the domestic cat, Felis catus, has been sequenced and conforms largely to the typical organization of previously characterized mammalian mtDNAs. Codon usage and base composition also followed canonical vertebrate patterns, except for an unusual ATC (non-AUG) codon initiating the NADH dehydrogenase subunit 2 (ND2) gene. Two distinct repetitive motifs at opposite ends of the control region contribute to the relatively large size (1559 bp) of this carnivore mtDNA. Alignment of the feline mtDNA genome to a homologous 7946-bp nuclear mtDNA tandem repeat DNA sequence in the cat, Numt, indicates simple repeat motifs associated with insertion/deletion mutations. Overall DNA sequence divergence between Numt and cytoplasmic mtDNA sequence was only 5.1%. Substitutions predominate at the third codon position of homologous feline protein genes. Phylogenetic analysis of mitochondrial gene sequences confirms the recent transfer of the cytoplasmic mtDNA sequences to the domestic cat nucleus and recapitulates evolutionary relationships between mammal species. 86 refs., 4 figs., 3 tabs.

  12. Ultrastructural study of the spermatozoon of Taenia taeniaeformis (Batsch, 1786) (Cestoda, Cyclophyllidea, Taeniidae), an intestinal parasite of Felis catus from La Palma (Canary Islands, Spain).

    Science.gov (United States)

    Miquel, Jordi; Foronda, Pilar; Torres, Jordi; Swiderski, Zdzisław; Feliu, Carlos

    2009-06-01

    The ultrastructural characters of the mature spermatozoon of Taenia taeniaeformis are described by means of transmission electron microscopy. Materials were obtained from a naturally infected road-killed cat (Felis catus) from La Palma (Canary Islands, Spain). The mature spermatozoon of T. taeniaeformis is a filiform cell, which is tapered at both extremities and lacks mitochondria. It is characterised by the presence of (1) a single spirallised crested body about 140 nm thick, (2) a single axoneme of the 9+'1' pattern of trepaxonematan Platyhelminthes, (3) a twisted (40 degrees ) layer of submembranous cortical microtubules, (4) a periaxonemal sheath surrounding the axoneme, (5) transverse intracytoplasmic walls and (6) a spirallised nucleus encircling the axoneme. The mature spermatozoon of T. taeniaeformis is also characterised by the presence of an apical cone in its anterior extremity and by the disorganisation of the axoneme in its posterior extremity. The ultrastructural characters of the mature spermatozoon of T. taeniaeformis are compared with those of other cestodes studied to date, with particular emphasis on other representatives of the family Taeniidae.

  13. Dietary canitine maintains energy reserves and delays fatigue of exercised african catfish (Clarias gariepinus fed high fat diets Carnitina dietética mantem reservas energéticas e evita a fatiga de bagre-africano durante exercício

    Directory of Open Access Journals (Sweden)

    Rodrigo Ozório

    2005-06-01

    Full Text Available Lipids, together with proteins, are traditionally considered as primary fuels during aerobic swimming. The effects of dietary fat and carnitine supplements and exercise on the energy metabolism of juvenile fish were investigated. One hundred African catfish (Clarias gariepinus were fed four isonitrogenous diets containing a fat level of 100 or 190 g kg-1 diet and one of the two levels of carnitine (15 and 1000 mg kg-1. Fish grew from 61 to 162 g in 10 wk. Thereafter, 6 fish per group swam vigorously for 3 h and the results were compared with unexercised groups. Fish receiving 1,000 mg carnitine accumulated 2- to 3-fold more carnitine than fish receiving 15 mg carnitine. Plasma acyl-carnitine level was affected by an interaction between dietary treatment and exercise (P Lipídios e proteínas são tradicionalmente considerados combustíveis primários durante natação aeróbica. Nesse ensaio foi investigado o efeito da suplementação de vários níveis de gordura e carnitina no metabolismo de 100 bagres africanos juvenis (Clarias gariepinus. Os peixes foram arraçoados com quatro dietas isoprotéicas, cada uma contendo 100 ou 190 g gordura kg-1 dieta, e um dos dois níveis de carnitina (15 e 1000 mg kg-1. Os peixes cresceram de 61 a 162 g em 10 semanas. No final do ensaio de alimentação, grupos de seis peixes por tratamento foram induzidos a nadar vigorosamente por 3 h e em seguida vários parâmetros foram determinados no tecido muscular e plasma, e os resultados observados nos grupos exercitados foram comparados com grupos controles (não exercitados. Os peixes arraçoados com 1,000 mg carnitina acumularam de duas a três vezes mais carnitina que os peixes arraçoados com 15 mg carnitina. O nível de acyl-carnitina no plasma foi influenciado pela interação entre os tratamentos dietéticos e exercício físico (P < 0.05. As concentrações de adenosina trifosfato (ATP e fosfocreatina no tecido muscular branco (WM foram mais elevadas em

  14. The biological and reproductive parameters of the invasive armored catfish Pterygoplichthys disjunctivus from Adolfo López Mateos El Infiernillo Reservoir, Michoacán-Guerrero, Mexico Parámetros biológicos y reproductivos del bagre armado invasivo Pterygoplichthys disjunctivus del embalse Adolfo López Mateos El Infiernillo, Michoacán-Guerrero, México

    Directory of Open Access Journals (Sweden)

    Rebeca Aneli Rueda-Jasso

    2013-03-01

    Full Text Available Invasive Pterygoplichthys disjunctivus (Weber, 1991 (synonymy: Liposarcus multiradiatus armored catfish were first reported in Mexico in 1995 and have spread successfully in several aquatic ecosystems. In Adolfo López Mateos El Infiernillo Reservoir, Michoacán - Guerrero, the population of this armored catfish is growing substantially because natural predators are not present and exploitation by humans does not occur. Over a complete annual cycle, we studied the following population parameters: weight, total and standard length, the relationship of weight to total length, the hepatosomatic (HSI and gonadosomatic (GSI indices, total fecundity (TF and relative fecundity (RF. The average total (TL and standard lengths (SL were 248 ± 37.5 and 188 ± 30.7 mm, respectively, and the average weight (W was 135.3± 66.8 g. The largest fish reported was 520 mm in TL and weighed 1 280 g. The species showed an extended reproductive season from May to November, as indicated by the GSI, TF and RF. The strongest reproductive peak occurred from July through October. During the annual cycle, the highest monthly average fecundity was 2 447 eggs, and the average relative fecundity was 12.6 eggs per g of fish (August and September. This information will be crucial for calculating the current biomass and future growth of the population. The calculation of these results could provide a basis for the exploitation of this resource for human consumption and animal feeds.La especie invasiva "bagre armado" P. disjunctivus (Weber 1991 (sinonimia de Liposarcus multuradiatus fue registrada por primera vez en México en 1995; en diversos sistemas acuáticos se ha distribuido exitosamente. En la presa Adolfo López Mateos El Infiernillo, Michoacán - Guerrero, la población de peces armados se ha incrementado considerablemente debido a la ausencia de depredadores y la falta de aprovechamiento. Por ello, a lo largo de un ciclo anual evaluamos los siguientes par

  15. The use of quantitative PCR to detect Felis catus papillomavirus type 2 DNA from a high proportion of queens and their kittens.

    Science.gov (United States)

    Thomson, N A; Dunowska, M; Munday, J S

    2015-02-25

    Squamous cell carcinomas are common feline skin cancers that have been associated with infection with Felis catus papillomavirus type 2 (FcaPV-2). Currently, little is known about the epidemiology of FcaPV-2 infection. The aim of this study was to develop a real-time PCR assay to quantify FcaPV-2 DNA in plucked hairs and skin swabs from 11 healthy breeding queens and their kittens. Samples were taken prior to kittening and then 2, 7 and 28 days after kittening to determine the age at which the kittens were first exposed to the virus. FcaPV-2 DNA was amplified from all of the queens and from 91% of the kittens at 2 days of age. There was a wide range in the quantity of FcaPV-2 DNA detected, from 1 to 92,520 copies per swab, and from 0.01 to 234 copies per copy of reference gene DNA in the hair plucks. The quantity of FcaPV-2 DNA detected in samples collected from the kittens was strongly correlated to that of their respective queens and the mean viral DNA load was similar for cats within a household but varied significantly between households. This is the first time that quantitative PCR has been used to detect FcaPV-2 DNA and the results suggest that the virus is ubiquitous but there is a wide variation of viral DNA loads. Kittens appear to be exposed to FcaPV-2 early in life, presumably from direct contact with their queen. These results are important when determining if FcaPV-2 infection of cats is preventable.

  16. Absence of zoonotic Bartonella species in questing ticks: First detection of Bartonella clarridgeiae and Rickettsia felis in cat fleas in the Netherlands

    Directory of Open Access Journals (Sweden)

    Reimerink Johan R

    2011-04-01

    Full Text Available Abstract Background Awareness for flea- and tick-borne infections has grown in recent years and the range of microorganisms associated with these ectoparasites is rising. Bartonella henselae, the causative agent of Cat Scratch Disease, and other Bartonella species have been reported in fleas and ticks. The role of Ixodes ricinus ticks in the natural cycle of Bartonella spp. and the transmission of these bacteria to humans is unclear. Rickettsia spp. have also been reported from as well ticks as also from fleas. However, to date no flea-borne Rickettsia spp. were reported from the Netherlands. Here, the presence of Bartonellaceae and Rickettsiae in ectoparasites was investigated using molecular detection and identification on part of the gltA- and 16S rRNA-genes. Results The zoonotic Bartonella clarridgeiae and Rickettsia felis were detected for the first time in Dutch cat fleas. B. henselae was found in cat fleas and B. schoenbuchensis in ticks and keds feeding on deer. Two Bartonella species, previously identified in rodents, were found in wild mice and their fleas. However, none of these microorganisms were found in 1719 questing Ixodes ricinus ticks. Notably, the gltA gene amplified from DNA lysates of approximately 10% of the questing nymph and adult ticks was similar to that of an uncultured Bartonella-related species found in other hard tick species. The gltA gene of this Bartonella-related species was also detected in questing larvae for which a 16S rRNA gene PCR also tested positive for "Candidatus Midichloria mitochondrii". The gltA-gene of the Bartonella-related species found in I. ricinus may therefore be from this endosymbiont. Conclusions We conclude that the risk of acquiring Cat Scratch Disease or a related bartonellosis from questing ticks in the Netherlands is negligible. On the other hand fleas and deer keds are probable vectors for associated Bartonella species between animals and might also transmit Bartonella spp. to humans.

  17. A new atypical genotype mouse virulent strain of Toxoplasma gondii isolated from the heart of a wild caught puma (Felis concolor) from Durango, Mexico.

    Science.gov (United States)

    Dubey, J P; Alvarado-Esquivel, C; Herrera-Valenzuela, V H; Ortiz-Diaz, J J; Oliveira, S; Verma, S K; Choudhary, S; Kwok, O C H; Su, C

    2013-11-08

    Nothing is known of the genetic diversity of Toxoplasma gondii circulating in wildlife in Mexico. In the present study, a mouse virulent T. gondii strain was isolated from the heart of a wild puma (Felis concolor). The puma was found roaming in outskirt of Durango City, Mexico and tranquilized for moving to a zoo. The puma died during translocation and a necropsy examination was performed. The puma had an antibody titer for T. gondii of 200 by the modified agglutination test. Its heart and brain tissue were bioassayed into 2 outbred Swiss Webster (SW) and 1 gamma interferon gene knockout (KO) mouse. The KO mouse and the 2 SW mice that became infected after inoculation with homogenate of puma heart died of acute toxoplasmosis 12, 19 and 20 days p.i. respectively and tachyzoites were found in lungs of all 3 mice. None of the 4 SW and 1 KO mouse inoculated with digest of the puma brain became infected with T. gondii. Tachyzoites from the lungs of mice were propagated in cell cultures. Tachyzoites from cell culture were inoculated into 5 SW; the mice died or had to be killed 14 days p.i. and a cat fed tissues of these mice shed T. gondii oocysts. Results of mortality and infectivity of tachyzoites and oocysts in SW mice indicated that the puma T. gondii strain (designated TgPumaMe1) was virulent for outbred mice. DNA isolated from culture-derived tachyzoites was characterized using 11 PCR-RFLP markers (SAG1, 5'- and 3'-SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico) revealed a new genotype (ToxoDB PCR-RFLP #222). Isolation of atypical genotype T. gondii from wild puma indicates that mouse virulent strains are circulating in wildlife in Mexico.

  18. Transforming properties of Felis catus papillomavirus type 2 E6 and E7 putative oncogenes in vitro and their transcriptional activity in feline squamous cell carcinoma in vivo

    Energy Technology Data Exchange (ETDEWEB)

    Altamura, Gennaro, E-mail: gennaro.altamura@unina.it [Department of Veterinary Medicine and Animal Productions, General Pathology and Pathological Anatomy Unit, University of Naples Federico II, Via Delpino 1, 80137 Naples (Italy); Corteggio, Annunziata, E-mail: ancorteg@unina.it [Department of Veterinary Medicine and Animal Productions, General Pathology and Pathological Anatomy Unit, University of Naples Federico II, Via Delpino 1, 80137 Naples (Italy); Pacini, Laura, E-mail: PaciniL@students.iarc.fr [Infections and Cancer Biology Group, International Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon (France); Conte, Andrea, E-mail: andreaconte88@hotmail.it [Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Via Pansini 5, 80131 Naples (Italy); Pierantoni, Giovanna Maria, E-mail: gmpieran@unina.it [Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Via Pansini 5, 80131 Naples (Italy); Tommasino, Massimo, E-mail: tommasinom@iarc.fr [Infections and Cancer Biology Group, International Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon (France); Accardi, Rosita, E-mail: accardir@iarc.fr [Infections and Cancer Biology Group, International Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon (France); Borzacchiello, Giuseppe, E-mail: borzacch@unina.it [Department of Veterinary Medicine and Animal Productions, General Pathology and Pathological Anatomy Unit, University of Naples Federico II, Via Delpino 1, 80137 Naples (Italy)

    2016-09-15

    Felis catus papillomavirus type 2 (FcaPV2) DNA is found in feline cutaneous squamous cell carcinomas (SCCs); however, its biological properties are still uncharacterized. In this study, we successfully expressed FcaPV2 E6 and E7 putative oncogenes in feline epithelial cells and demonstrated that FcaPV2 E6 binds to p53, impairing its protein level. In addition, E6 and E7 inhibited ultraviolet B (UVB)-triggered accumulation of p53, p21 and pro-apoptotic markers such as Cleaved Caspase3, Bax and Bak, suggesting a synergistic action of the virus with UV exposure in tumour pathogenesis. Furthermore, FcaPV2 E7 bound to feline pRb and impaired pRb levels, resulting in upregulation of the downstream pro-proliferative genes Cyclin A and Cdc2. Importantly, we demonstrated mRNA expression of FcaPV2 E2, E6 and E7 in feline SCC samples, strengthening the hypothesis of a causative role in the development of feline SCC. - Highlights: • FcaPV2 E6 binds to and deregulates feline p53 protein. • FcaPV2 E7 binds to and deregulates feline pRb protein. • FcaPV2 oncogenes inhibit UVB-induced apoptosis. • FcaPV2 E6E7 and E7 increase the lifespan of primary cells. • FcaPV2 E2, E6 and E7 are expressed at the mRNA level in feline SCC in vivo.

  19. Synthèse et étude structrale de lyonsite-type (Na0,4,Li0,6(Fe,Li2(MoO43

    Directory of Open Access Journals (Sweden)

    Amira Souilem

    2015-06-01

    Full Text Available The new compound (Na0.4,Li0.6(Fe,Li2(MoO43 was synthesized by cooling from the melt. Its anionic framework is built up from two distinct MO6 octahedra, each containing disordered Li+ and Fe3+ ions in 0.6:0.4 and 0.7:0.3 ratios, and two MoO4 tetrahedra, which link by vertex-sharing of their O atoms. These tetrameric units are further linked by sharing edges between octahedra and by formation of M—O—Mo (M = Fe/Li bridges, forming ribbons propagating in the [100] direction. The ribbons are cross-linked in both the b- and c-axis directions, giving rise to a three-dimensional framework having [100] tunnels in which the monovalent Na+/Li+ cations (0.4:0.6 ratio lie. Bond-valence calculations are consistent with the disorder model for the cations. The structure of the title compound, which is isotypic with Li3Fe(MoO43 and Li3Ga(MoO43, is compared briefly with those of LiFeMo2O8 and Li1.6Mn2.2(MoO43.

  20. Sanitary conditions of a colony of urban feral cats (Felis catus Linnaeus, 1758 in a zoological garden of Rio de Janeiro, Brazil Condições sanitárias de uma colônia urbana de gatos (Felis catus Linnaeus, 1758 em um jardim zoológico do Rio de Janeiro, Brasil

    Directory of Open Access Journals (Sweden)

    Flavya Mendes-de-Almeida

    2004-10-01

    Full Text Available The colony of urban stray cats living in the Rio de Janeiro zoological garden was studied in order to develop a population and health control program. As many cats as possible were captured during two months (47 animals and were classified according to gender, age, weight and coat markings. They were submitted to a general health evaluation, examined for the presence of ectoparasites and sent to a surgical neutering program. All animals had a blood sample drawn for CBC, platelet count, heartworm and retroviruses detection. Capillary blood smears were made for hemoparasites detection. Coat marking and colors were tabby (59.7%, followed by solid black (17%; torbie (10.6%; bicolor (10.6% and harlequin (2.1%. The only ectoparasites found were fleas, which infested 28% of the animals. The hemoparasites found were Haemobartonella felis (38% and piroplasmas that could not be differentiated between Cytauxzoon spp. and Babesia spp. (47%. No cat was found infected by Dirofilaria immitis or FeLV (Feline Leukemia Virus, although FIV (Feline Immunodeficiency Virus antibodies could be detected (21%. There was no correlation between hemoparasites and FIV infections. The estimated total cat population (mark-recapture method was 59; 68% female and 32% male, suggesting that a neutering program is in fact needed.As condições sanitárias e composição populacional de uma colônia de gatos urbanos, errantes, habitantes do zoológico do Rio de Janeiro foram estudadas, objetivando-se um programa de controle populacional e sanitário. Capturou-se o maior número de indivíduos possível durante dois meses (47 animais. Os animais capturados foram examinados quanto ao gênero, idade, peso, pelagem, inspeção geral e presença de ectoparasitas e eram encaminhados a um programa de esterilização cirúrgica. Cada animal teve uma amostra de sangue colhida para realização de hemograma completo, plaquetometria, pesquisa de hemoparasitas e de retrovírus. As marca

  1. Efficacy of slow-release collar formulations of imidacloprid/flumethrin and deltamethrin and of spot-on formulations of fipronil/(s - methoprene, dinotefuran/pyriproxyfen/permethrin and (s –methoprene/amitraz/fipronil against Rhipicephalus sanguineus and Ctenocephalides felis felis on dogs

    Directory of Open Access Journals (Sweden)

    Horak Ivan G

    2012-04-01

    Full Text Available Abstract Background Two studies evaluating the efficacy of an imidacloprid/flumethrin collar (Seresto®, Bayer Animal Health, IVP, a deltamethrin collar (Scalibor®, MSD, CP1, a fipronil/(s-methoprene spot-on (Frontline Combo®, Merial, CP2, a dinotefuran/pyriproxyfen/permethrin spot-on (Vectra 3D®, Ceva, CP3 and an amitraz/fipronil/(s-methoprene spot-on (Certifect®, Merial, CP4/CP5 against repeated infestations with Rhipicephalus sanguineus and Ctenocephalides felis felis on dogs were conducted over periods of 226 days and 71 days respectively. Methods The first study comprised 4 groups of treated dogs and one untreated control group, and the second 3 groups of treated dogs and one control group. Each group consisted of 8 dogs. All dogs were infested with ticks and fleas at regular intervals. Ticks were counted 6 h, 18 h or 48 h after infestations and fleas 24 h after infestations. Efficacies of the treatments were calculated by comparison with the untreated control groups using standard descriptive statistics. Results The protective 48 h tick efficacy was 97.8% to 100% for the IVP (226 days, 69.3% to 97.4% for CP1 (170 days, 99.6% to 43.4% for CP2 (35 days and 98% to 61.4% for CP3 (35 days. The protective 18 h tick efficacy was 98% to 99.6% for the IVP (71 days, 100% to 86.5% for CP4 (29 days, 100% to 72.8% for CP4 after re-treatment (35 days and 98.8% to 54.3% for CP5 (35 days. The protective 6 h tick efficacy was 85.6% at Day 7 and 90.1% to 97.1% from Day 14 onwards for the IVP (70 days, 92.3% to 70.7% for CP4 (35 days, 97.5% to 65.2% for CP4 after re-treatment (35 days and 95.1% to 51.8% for CP5 (35 days. The protective 24 h flea efficacy was 99.5/90.9% to 100% for the IVP (71/226 days, 66.7% to 83% for CP1 (170 days, 100% to 88.5% for CP2 (35 days, 100% to 73.3% for CP3 (35 days, 100% to 98.7% for CP4 (35 days, 100% to 87.5% for CP4 after re-treatment (35 days and 100% to 79.5% for

  2. Spotlight census of the red fox (Vulpes vulpes and the domestic cat (Felis catus in three sample areas of the Marches region (Central Italy / Censimento notturno di Volpe (Vulpes vulpes e di Gatto domestico (Felis catus in tre aree campione delle Marche

    Directory of Open Access Journals (Sweden)

    Massimo Pandolfi

    1991-07-01

    Full Text Available Abstract In order to evaluate the density of the red fox and of the domestic cat, 55 transects were made from 1986 to 1989 using spotlight census method in three sample areas. The mean density of foxes agreed substantially with its biological cycle and the hightes values (2.01 foxes/km² in spring and 4.3 foxes/km² in winter were recorded in the study area with the better natural characteristics. Foxes selected the shrub woodland (macchia all year round, the inhabited area in spring. The domestic cat was widely spread and abundant, and selected especially inhabited areas where the density varied from 4.27 cats/km² (in winter to 12.42 cat/km² (in spring. Riassunto Dal 1986 al 1989, con il metodo dei percorsi notturni con fari, sono stati effettuati complessivamente 55 conteggi in tre aree campione per valutare la densità della Volpe (Vulpes vulpes e del Gatto domestico (Felis catus nonché le loro preferenze ambientali limitatamente ad una zona campione. Per la Volpe le densità medie rilevate sono sostanzialmente in accordo con il ciclo biologico della specie e quelle più elevate (2,O1 volpi/km² in primavera e 4,3 volpi/km² in inverno sono state registrate nella zona campione con maggior presenza di boschi ed aree incolte. La Volpe seleziona le zone con vegetazione "di macchia" in ogni periodo dell'anno, e le aree abitate in primavera. Per il Gatto domestico le densità rilevate evidenziano la presenza di una diffusa ed abbondante popolazione. La specie mostra una spiccata preferenza per le aree abitate dove raggiunge densità di 4,27 individui/km² e 12,42 individui/km² in inverno e primavera rispettivamente.

  3. 猞猁和豹猫肝脏结构的观察比较及AQP -8在肝脏中的表达%On Liver Structure and Expression of AQP - 8 in Felis lynx and Prionailurus bengalensis liver

    Institute of Scientific and Technical Information of China (English)

    王昱

    2015-01-01

    The liver structure of Felis lynx and Prionailurus bengalensis was compared with light microscope and expression of aquaporin - 8(AQP - 8)was measured by immunohistochemistry. The results show that the basic structure of the livers of Felis lynx and Prionailurus bengalensis was similar with the basic structure of most mammalian livers. The outer layer of the liver was a connective tissue capsule. The hepatic lobules were unclear. The hepatic cords,hepatic sinusoids and space of Disse arranged radially around a central vein and formed an ex-tensive spongework. The hepatic cords were comprised one row of hepatocytes. Interlobular artery,interlobular vein and interlobular bile duct in portal area were observed easily. The hepatic lobules of Prionailurus bengalensis were more regular than those of Felis lynx,but the diameter of hepatocytes of Prionailurus bengalensis was smaller than that of Felis lynx. The adjacent hepatocytes in the hepatic cords of Felis lynx were loose,the hepatic sinu-soids were very abundant. The expression of AQP - 8 in Felis lynx liver was stronger than that in Prionailurus ben-galensis liver. These indicate that the liver structure of Felis lynx and Prionailurus bengalensis and expression of AQP - 8 are adaptable to the life habit.%利用生物显微技术对猞猁(Felis lynx)和豹猫(Prionailurus bengalensis)肝脏进行了比较,应用免疫组织化学方法检测了水通道蛋白-8(AQP -8)在肝脏中的表达情况。结果显示,与多数哺乳类相似,猞猁和豹猫的肝脏外被覆一层结缔组织薄膜,肝小叶的界限不清楚,中央静脉周围放射状排列着肝板、肝血窦及狄氏间隙,单层排肝细胞构成肝板,在门管区可清晰观察到小叶间静脉、小叶间动脉和小叶间胆管豹猫肝小叶的肝细胞排列较猞猁的规则,但肝细胞的直径较猞猁的小。猞猁肝细胞间比较松散,肝血窦较豹猫的发达。猞猁肝脏 AQP -8的表达较豹猫的

  4. Five-month comparative efficacy evaluation of three ectoparasiticides against adult cat fleas (Ctenocephalides felis), flea egg hatch and emergence, and adult brown dog ticks (Rhipicephalus sanguineus sensu lato) on dogs housed outdoors.

    Science.gov (United States)

    Varloud, Marie; Hodgkins, Elizabeth

    2015-03-01

    This study was designed to compare the efficacy of three topical combinations on dogs in outdoor conditions against adult cat fleas (Ctenocephalides felis), flea egg hatch and emergence, and against adult brown dog ticks (Rhipicephalus sanguineus sensu lato). Treatment was performed on day 0 with a placebo; dinotefuran, pyriproxifen and permethrin (DPP); fipronil and (S)-methoprene (FM) or imidacloprid and permethrin (IP). Dogs (n = 32), housed outdoors for 7 months, were treated monthly for four consecutive months (on days 0, 30, 60 and 90) and infested with ~100 unfed adult fleas on days 14, 55, 74, 115 and 150 and with ~50 unfed adult ticks on days 28, 44, 88 and 104. Adult fleas were counted and removed 24 h after infestation. Immediately after flea removal, dogs were reinfested with ~100 new adult fleas 72 h prior to egg collection for up to 48 h. Flea eggs were incubated for 32 days, and newly emerged adults were counted. Ticks were counted and removed 48 h after each infestation. FM had >90 % efficacy against fleas at each time point and variable efficacy against ticks (38.0-99.6 %). Efficacy of IP was 60 days after the last treatment. Despite challenging weather conditions, DPP was highly effective, providing >90 % efficacy against adult ticks as well as adult and immature fleas at every time point of the study.

  5. 猞猁肝脏的组织结构观察及EGF在肝脏中的表达%Observations on Histological Structure and Expression of EGF in Liver of Felis lynx

    Institute of Scientific and Technical Information of China (English)

    王昱

    2011-01-01

    利用切片方法观察了猞猁Felis lynx肝脏的组织结构,应用免疫组织化学方法检测了表皮生长因子(EGF)在肝脏中的表达.结果显示,肝脏外被覆一层结缔组织薄膜,肝小叶不规则且分界不清,肝板、肝血窦及狄氏间隙围绕中央静脉呈放射状排列,肝板由一排肝细胞构成.肝细胞呈圆形或多边形,多为单核,少数具双核,肝细胞间比较松散.肝血窦发达,内可见血细胞.EGF阳性反应主要定位于肝细胞质中,表明EGF可能参与细胞新陈代谢过程的调控.

  6. Comparative efficacy on dogs of a single topical treatment with fipronil/(S-methoprene or weekly physiological hygiene shampoos against Ctenocephalides felis in a simulated flea-infested environment

    Directory of Open Access Journals (Sweden)

    Beugnet F.

    2012-05-01

    Full Text Available Flea infestations of pets continue to persist due to the lack of knowledge of flea biology and ecology. It is not unusual that pet owners believe regular hygiene, such as shampooing their dogs can replace regular insecticidal treatment. The objective of this study was to compare in a flea simulated environment, modelling exposure similar to that found in a home, that the use of regular physiological shampoo does not control fleas adequately when compared to a long acting topical formulation. Three groups of six dogs were formed: one untreated control group, one group treated monthly with the topical formulation of fipronil/(S-methoprene, and a third group treated weekly with a hygiene shampoo. All dogs were infested with adult unfed Ctenocephalides felis fleas (200 ± 5 on Days -28 and -21. Each animal’s sleeping box was fitted with a plastic cup mounted to the inside roof of the box. The sleeping bench of each animal was covered with a carpet to accommodate flea development. The dogs were maintained in their kennels throughout the study. In order to maintain the environmental flea challenge, C. felis pupae (100 ± 5 were placed in the plastic cup in each animal’s sleeping box on Days -14, -7, 0, 7, 14, 21, 28, 35 and 42. The dogs were combed and fleas counted weekly on Days -1, 3, 10, 17, 24, 31, 38, 45, and 51. The fleas were placed immediately back on the dogs. On Day 60, fleas were counted and removed. Flea infestations in the untreated control group at each count averaged between 46.2 and 74.2 fleas throughout the study. The average number of fleas infesting dogs was significantly different (p < 0.05 between the untreated and the two treatment groups and between the two treatment groups at all counts throughout the two months study (Days 3, 10, 17, 24, 31, 38, 45, 51 and 60. The efficacy was never below 99.1% in the fipronil/(S-methoprene group, and efficacy in the shampoo group was never above 79.2%. Weekly shampooing in treatment

  7. Epidemiology of Sarcocystis neurona infections in domestic cats (Felis domesticus) and its association with equine protozoal myeloencephalitis (EPM) case farms and feral cats from a mobile spay and neuter clinic.

    Science.gov (United States)

    Stanek, J F; Stich, R W; Dubey, J P; Reed, S M; Njoku, C J; Lindsay, D S; Schmall, L M; Johnson, G K; LaFave, B M; Saville, W J A

    2003-11-28

    Equine protozoal myeloencephalitis (EPM) is a serious neurologic disease in the horse most commonly caused by Sarcocystis neurona. The domestic cat (Felis domesticus) is an intermediate host for S. neurona. In the present study, nine farms, known to have prior clinically diagnosed cases of EPM and a resident cat population were identified and sampled accordingly. In addition to the farm cats sampled, samples were also collected from a mobile spay and neuter clinic. Overall, serum samples were collected in 2001 from 310 cats, with samples including barn, feral and inside/outside cats. Of these 310 samples, 35 were from nine horse farms. Horse serum samples were also collected and traps were set for opossums at each of the farms. The S. neurona direct agglutination test (SAT) was used for both the horse and cat serum samples (1:25 dilution). Fourteen of 35 (40%) cats sampled from horse farms had circulating S. neurona agglutinating antibodies. Twenty-seven of the 275 (10%) cats from the spay/neuter clinic also had detectable S. neurona antibodies. Overall, 115 of 123 (93%) horses tested positive for anti-S. neurona antibodies, with each farm having greater than a 75% exposure rate among sampled horses. Twenty-one opossums were trapped on seven of the nine farms. Eleven opossums had Sarcocystis sp. sporocysts, six of them were identified as S. neurona sporocysts based on bioassays in gamma-interferon gene knockout mice with each opossum representing a different farm. Demonstration of S. neurona agglutinating antibodies in domestic and feral cats corroborates previous research demonstrating feral cats to be naturally infected, and also suggests that cats can be frequently infected with S. neurona and serve as one of several natural intermediate hosts for S. neurona.

  8. Assessment of the efficacy of a topical combination of fipronil-permethrin (Frontline Tri-Act®/Frontect® against egg laying and adult emergence of the cat flea (Ctenocephalides felis in dogs

    Directory of Open Access Journals (Sweden)

    Beugnet Frédéric

    2016-01-01

    Full Text Available This study was conducted to assess the prevention of egg laying and the inhibition of the emergence of the cat flea (Ctenocephalides felis resulting from the application of a combination of fipronil and permethrin (Frontline Tri-Act®/Frontect®, Merial on dogs. Sixteen healthy dogs were included after pre-treatment live flea counts and randomly allocated to two groups. Eight dogs served as untreated controls and 8 dogs were treated on Day 0 and Day 30 with topical application of fipronil/permethrin at the minimum dose of 6.76 mg/kg fipronil and 50.48 mg/kg permethrin. On days −2, 7, 21, 28, 42 and 56, each dog was infested with 100 fleas. Flea eggs were collected from each dog in individual trays from 12 to 36 h after treatment or each flea re-infestation. All fleas were removed by combing and counted 36 h after treatment or infestations. The collected eggs were counted and incubated for 28 days for larval development and adult emergence assessment. The curative efficacy of Frontline Tri-Act®/Frontect® against adult fleas 36 h after treatment was 95.3% and the efficacy remained 100% after subsequent flea infestations for 8 weeks. Compared to the control group, the treatment reduced egg laying by 84.5% within 36 h after first treatment and was 99.9%, 100%, 100%, 100%, 100% on collection days 7, 21, 29, 43 and 57, respectively. Frontline Tri-Act®/Frontect® reduced by 28.7% the emergence of new adult fleas from eggs laid during the 48 h of pre-treatment infestation. The inhibition of adult emergence from incubated flea eggs could not be assessed after flea re-infestation in the treated group as no eggs were collected.

  9. HÁBITOS ALIMENTARIOS DEL BARBUL DE PIEDRA (Ariopsis sp. EN EL RÍO SINÚ, COLOMBIA Feeding Habits of Barbul de Piedra (Ariopsis sp. in the Sinu River, Colombia

    Directory of Open Access Journals (Sweden)

    CHARLES W OLAYA-NIETO

    2012-04-01

    Full Text Available Se estudiaron los hábitos alimentarios del barbul de piedra en el río Sinú. El contenido estomacal se evaluó con el coeficiente de vacuidad, grado de digestión, frecuencia de ocurrencia, frecuencia numérica, gravimetría, índice de importancia relativa y relación longitud intestinal-longitud total. Solo 18,8% de los estómagos se encontró vacío, la mayoría de las presas estaban medio digeridas y se identificaron cinco grupos alimentarios: crustáceos, peces, material vegetal, moluscos y otros. Crustáceos fue el grupo más frecuente, abundante y con mayor composición en peso. El índice de importancia relativa indicó que crustáceos y peces son grupos de importancia relativa secundaria, mientras que restos vegetales, moluscos y otros fueron ocasionales o circunstanciales. Los resultados alcanzados en este trabajo muestran que el barbul de piedra es un pez que mantiene sus hábitos alimentarios a medida que va creciendo y a lo largo del ciclo hidrológico, aunque muestra preferencia carnívora, especialmente por los Crustáceos.The feeding habits of Barbul de piedra in the Sinu river were studied. The stomach content was analyzed using the proportion of empty stomachs, grade of digestion, frequency of occurrence, numerical frequency, gravimetry, relative importance index and the gut length-total length relationship. Only 18.8% of stomachs were empty, most of preys were half-digested and five food groups were identified: Crustaceans, fishes, vegetable rests, molluscs and others. Crustaceans were the most frequent, abundant and greater weight composition group. The relative importance index indicated that crustaceans and fishes were secondary relative importance groups and vegetable rests, molluscs and others were occasional or circumstantial groups. The results achieved in this work indicate that Barbul de piedra is a fish that keeps your feeding habits as they grow and along the hydrological cycle, although shows carnivorous preference, especially by Crustaceans.

  10. HÁBITOS ALIMENTARIOS DEL BARBUL DE PIEDRA (Ariopsis sp. EN EL RÍO SINÚ, COLOMBIA

    Directory of Open Access Journals (Sweden)

    CHARLES W. OLAYA-NIETO

    2012-01-01

    Full Text Available Se estudiaron los hábitos alimentarios del Barbul de piedra en el río Sinú. El contenido estomacal se evaluó con el Coeficiente de vacuidad, Grado de digestión, Frecuencia de ocurrencia, Frecuencia numérica, Gravimetría, Índice de importancia relativa y la relación longitud intestinal-longitud total. Solo el 18,8% de los estómagos se encontró vacío, la mayoría de las presas estaban medio digeridas y se identificaron cinco grupos alimentarios: Crustáceos, Peces, Material vegetal, Moluscos y Otros. Crustáceos fue el grupo más frecuente, abundante y con mayor composición en peso. El Índice de importancia relativa indicó que Crustáceos y Peces son grupos de importancia relativa secundaria, mientras que Restos vegetales, Moluscos y Otros fueron ocasionales o circunstanciales. Los resultados alcanzados en este trabajo muestran que el Barbul de piedra es un pez con hábitos alimentarios carnívoros con preferencia por Crustáceos.

  11. Hábitos alimentarios del Barbul de piedra (Ariopsis bonillai en el río Sinú, Colombia

    Directory of Open Access Journals (Sweden)

    Arellano Padilla John Jairo

    2012-04-01

    Full Text Available Se estudiaron los hábitos alimentarios del Barbul de piedra en el río Sinú. El contenido estomacal se evaluó con el Coeficiente de vacuidad, Grado de digestión, Frecuencia de ocurrencia, Frecuencia numérica, Gravimetría, Índice de importancia relativa y la relación longitud intestinal-longitud total. Solo el 18,8% de los estómagos se encontró vacío, la mayoría de las presas estaban medio digeridas y se identificaron cinco grupos alimentarios: Crustáceos, Peces, Material vegetal, Moluscos y Otros. Crustáceos fue el grupo más frecuente, abundante y con mayor composición en peso. El Índice de importancia relativa indicó que Crustáceos y Peces son grupos de importancia relativa secundaria, mientras que Restos vegetales, Moluscos y Otros fueron ocasionales o circunstanciales. Los resultados alcanzados en este trabajo muestran que el Barbul de piedra es un pez con hábitos alimentarios carnívoros con preferencia por Crustáceos.

  12. Toxoplasmosis in Sand cats (Felis margarita) and other animals in the Breeding Centre for Endangered Arabian Wildlife in the United Arab Emirates and Al Wabra Wildlife Preservation, the State of Qatar.

    Science.gov (United States)

    Dubey, J P; Pas, An; Rajendran, C; Kwok, O C H; Ferreira, L R; Martins, J; Hebel, C; Hammer, S; Su, C

    2010-09-20

    The Sand cat (Felis margarita) is a small-sized felid found in sand and stone deserts ranging from the north of Africa to Asia, with the Arabian Peninsula as its centre of distribution. The Sand cat captive breeding program at the Breeding Centre for Endangered Arabian Wildlife (BCEAW), Sharjah, UAE, has experienced high newborn mortality rates, and congenital toxoplasmosis was recently recognized as one of the causes of this mortality. In the present study, one 18-month-old Sand cat (FM019) died of acute toxoplasmosis-associated hepatitis and pneumonitis acquired after birth; Toxoplasma gondii was demonstrated in histological sections which reacted with T. gondii polyclonal antibodies by immunohistochemistry (IHC). T. gondii DNA was found by PCR of extracted DNA from liver and lung tissues of this cat. Antibodies to T. gondii were found in serum examined in 1:1600 dilution in the modified agglutination test (MAT); its 2-year-old cage mate seroconverted (MAT titer 1:3200) at the same time. Another Sand cat (FM017) was euthanized because of ill health when 3 years old; its MAT titer was >1:3200, and T. gondii tissue cysts were found in brain, heart, ocular muscles and skeletal muscle, confirmed by IHC. Viable T. gondii was isolated by bioassays in mice inoculated with tissues of another chronically infected Sand cat (FM002); T. gondii was not found in histological sections of this cat. T. gondii antibodies were found in several species of animals tested, notably in 49 of 57 wild felids at BCEAW. A 7-year-old Sand cat (3657) from Al Wabra Wildlife Preservation (AWWP), Doha, State of Qatar died of acute visceral toxoplasmosis with demonstrable T. gondii tachyzoites by IHC, and T. gondii DNA by PCR, and a MAT titer of >3200. T. gondii antibodies were found in 21 of 27 of wild felids at AWWP. PCR-RFLP genotyping at 10 genetic loci revealed that these T. gondii isolates from Sand cat (FM002 and FM019) at BCEAW have an atypical genotype, which was previously reported in T

  13. Apparent total tract energy and macronutrient digestibility of one- to three-day-old, adult ground, extruded, and canned chicken-based diets in domestic cats (Felis silvestris catus).

    Science.gov (United States)

    Kerr, K R; Morris, C L; Burke, S L; Swanson, K S

    2014-08-01

    There has been a recent increase in the popularity of feeding unconventional diets, including whole prey diets, to domestic cats (Felis silvestris catus). Data are needed that allow animal caretakers to choose and formulate diets that meet the nutritional requirements of their cats. Our objective was to evaluate the effects of feeding 1- to 3-d-old whole chicks (WHO), ground adult chicken product (GRO), a chicken-based canned diet (CAN), and a chicken-based extruded diet (EXT) on apparent total tract energy and macronutrient digestibility, N balance, and blood metabolites of domestic cats (n = 11). Macronutrient, energy, and moisture concentrations of diets varied greatly (e.g., CP: 35 to 72% DM); however, cats fed all diets maintained BW and N balance. In general, cats fed WHO had lower nutrient digestibility than those fed CAN and EXT. Cats fed GRO had greater nutrient digestibility than cats fed commercial diets. For example, apparent OM and GE digestibility coefficients were greater (P ≤ 0.05) for cats fed CAN (86 and 88%, respectively), EXT (88 and 88%), and GRO (94 and 95%) compared with those fed WHO (83 and 83%) and greater (P ≤ 0.05) for cats fed GRO compared with those fed CAN and EXT. Many blood metabolites were modified by diet, but most remained within reference ranges for domestic cats. Serum cholesterol was elevated above the reference range for all treatments and greater (P ≤ 0.05) for cats fed WHO compared with those fed CAN, EXT, and GRO. Serum creatinine concentrations were above the reference range for all treatments and greater (P ≤ 0.05) for cats fed GRO compared with those fed CAN or WHO. These data indicate that the whole prey tested herein maintained short-term health and are adequately digestible for use in companion animal diets. Research is needed to determine the global and long-term health implications of feeding whole or ground diets to domestic cats, which may be different in terms of macronutrient, energy, and moisture

  14. Human Infection with Rickettsia felis, Kenya

    Science.gov (United States)

    2010-07-01

    Prescribed by ANSI Std Z39-18 RESEARCH rickeltsiosis, brucellosis . leptospirosis, and other viral in- fections. The study protocol was approved by the...sheep, goats , and camels; and carries a wide range of wi ldl ife. including zebras, ante- lopes. waterbucks. giraffes, warthogs, monkeys. gerenuks...contact with livestock animals such as cattle, sheep, goats , or camels. Although this was not captured in the question- naire, almost all livestock

  15. Estudo microscópico e macroscópico, com enfoque radiográfico e de alizarina, no desenvolvimento embrionário e fetal de gatos domésticos (Felis catus em diferentes idades gestacionais

    Directory of Open Access Journals (Sweden)

    Dilayla K Abreu

    2011-12-01

    Full Text Available O gato doméstico (Felis catus foi nomeado por Carolus Linnaeus em seu livro Systema Naturae, em 1798. A família Felidea apresenta muita semelhança morfológica com os felinos selvagens. O estudo da embriologia do gato doméstico é de grande valia, uma vez que, é considerado um importante modelo animal quando comparado aos gatos selvagem em extinção, especialmente relacionado às pesquisas sobre biologia reprodutiva. Este trabalho objetivou análisar e comparar as fases embrionárias de quatro embriões e um feto de felinos domésticos. Nos embriões com idade gestacional estimada em 17 dias (0,5cm CR podemos observar pela análise macroscópica a presença de dilatação rostral correspondente ao prosencéfalo, o local placóide do cristalino, a flexura cervical, os quatro arcos faríngeos com os sulcos que o dividem, a proeminência cardíaca, o indício do brotamento do membro pélvico, além da presença de somitos. Na região caudal do embrião, visualizamos a curvatura cranio-caudal, permitindo ao mesmo uma posição em formato de "C". Nos embriões com idade gestacional estimada em 22 dias (1,2cm CR, na análise macroscópica foi visualizado o prosencéfalo, vesícula óptica com pigmentação da retina, vesícula ótica, quarto ventrículo, fígado, membros torácicos e pélvicos com discreta distinção dos dígitos e vascularização superficial. Nos embriões com idade gestacional estimada em 25 dias (1,5cm CR notamos a presença do prosencéfalo e mesencéfalo, a curvatura cervical pronunciada, vesícula óptica com forte pigmentação da retina, vesícula ótica, membros pélvicos e torácicos bem desenvolvidos, com distinção dos dígitos e fígado bem pronunciado. Os fetos com idade gestacional estimada em 52 dias (10cm CR possuem estruturas internas e externas facilmente identificadas em animais adultos. Com relação às estruturas ósseas notamos que as mesmas não apresentam nenhuma epífise óssea formada, sendo vis

  16. Determination of cation distribution in the Fe[Li1/2Fe3/2]O4-LiFeTiO4-Li[Li1/3Ti5/3]O4 system: Mixed nature of solid solution and superlattice

    Science.gov (United States)

    Mukai, Kazuhiko; Kato, Yuichi; Nakano, Hideyuki

    2017-03-01

    The solid solution technique is one of the oldest but most powerful methods in the search for advanced materials. Hume-Rothery rules regarding solid solutions are well established for alloys, but little is known about oxides. In this study, we focused on the spinel oxides of Fe[Li1/2Fe3/2]O4-LiFeTiO4-Li[Li1/3Ti5/3]O4 to clarify their cation distribution, which necessitates other rules for substitutionally ordered/disordered solid solutions. Here, the chemical formula of the spinels is represented as Li1/2+x/2Fe5/2-3x/2TixO4 with 0≤x≤5/3. Synchrotron radiation X-ray diffraction measurements indicated two types of 1:3 cation order at the octahedral site over the wide x range; i.e., the cation order between one Li+ ion and three Fe3+ ions at 0≤x≤1 and the cation order between one Li+ ion and three Ti4+ ions at 1

  17. EVALUACIÓN DE ETILENGLICOL COMO CRIOPROTECTOR EN LA CRIOCONSERVACIÓN DE SEMEN DE BAGRE BLANCO (Sorubim cuspicaudus, PIMELODIDAE

    Directory of Open Access Journals (Sweden)

    VÍCTOR J. ATENCIO-GARCÍA

    2014-01-01

    Full Text Available Se evaluó el semen crioconservado de Sorubim cuspicaudus utilizando etilenglicol (ETG a tres niveles de inclusión (5, 10, 15 %. Machos (n = 13 en fase de espermiación y hembras (n = 6 en maduración final se indujeron con 0,4 ml de Ovaprim®/Kg, después de 12 a 14 horas post-inducción se colectó el semen en viales Eppendorf de 2 ml de capacidad. Las diferentes soluciones crioprotectoras se prepararon con glucosa 6 % (p/v, leche en polvo descremada 5 % (pv y agua destilada. El semen fue diluido en proporción 1:3 (semen:diluyente, empacado en macrotubos de 2,5 ml y congelado en vapores de nitrógeno líquido (NL durante 30 minutos y luego almacenados en termos criogénicos sumergidos directamente en NL (-196 °C. El semen crioconservado fue descongelado en baño serológico a 35 °C durante 90 segundos. La movilidad total, progresividad y velocidad espermática del semen fresco y descongelado se analizó con el software Sperm Class Analizer SCA® (Microptic SL, España. La fertilidad y eclosión se evaluó con 1,0-1,5 g de ovocitos en incubadoras experimentales de flujo ascendente de dos litros de capacidad. Se utilizó un diseño completamente aleatorizado. El semen fresco registró tasa de eclosión de 51,8±21 %, sin observarse diferencia significativa con la obtenida con el semen crioconservado con ETG 5 % (38,6 ± 13,9 % (p> 0,05; mientras que ETG 15 % (9,6 ± 2,9 % reportó la menor eclosión (p <0,05. Los resultados sugieren que la solución crioprotectora compuesta por ETG 5 %, glucosa 6 % y leche en polvo 5 % es una alternativa viable para la criocon- servación de semen de Sorubim cuspicaudus con fecundaciones similares al usar semen fresco.

  18. Aspectos biometricos del bagre Arius Furthii Steindachner (Pisces Ariidae, colectado en punta Morales, Golfo de Nicoya, Costa Rica

    Directory of Open Access Journals (Sweden)

    Maurizio Protti Quesada

    2016-03-01

    Full Text Available Se analizan estadísticamente algunos parámetros biométricos de 257 ejemplares de Arius furthii (Ariidae, colectados en una zona de playa y otra de manglar en Punta Morales, Puntarenas, Costa Rica. Las colectas se realizaron por medio de “arrastres” con una red tipo “chinchorro”. La talla de los especímenes capturados osciló entre 55 y 276 mm de longitud total y 1,3 a 176 g en peso fresco. El análisis estadístico mostró que la longitud total es la mejor variable para la estimación del peso fresco a través de una ecuación de regresión logarítmica.  Además, se mostró que no existen diferencias significativas en el peso de los Arius furthii colectados en la playa y los colectados en el manglar.

  19. Lateral bias and temperament in the domestic cat (Felis silvestris).

    Science.gov (United States)

    McDowell, Louise J; Wells, Deborah L; Hepper, Peter G; Dempster, Martin

    2016-11-01

    Research points to a relationship between lateralization and emotional functioning in humans and many species of animal. The present study explored the association between paw preferences and emotional functioning, specifically temperament, in a species thus far overlooked in this area, the domestic cat. Thirty left-pawed, 30 right-pawed, and 30 ambilateral pet cats were recruited following an assessment of their paw preferences using a food-reaching challenge. The animals' temperament was subsequently assessed using the Feline Temperament Profile (FTP). Cats' owners also completed a purpose-designed cat temperament (CAT) scale. Analysis revealed a significant relationship between lateral bias and FTP and CAT scale scores. Ambilateral cats had lower positive (FTP+) scores, and were perceived as less affectionate, obedient, friendly, and more aggressive, than left or right-pawed animals. Left and right pawed cats differed significantly on 1 trait on the CAT scale, namely playfulness. The strength of the cats' paw preferences was related to the animals' FTP and CAT scores. Cats with a greater strength of paw preference had higher FTP+ scores than those with a weaker strength of paw preference. Animals with stronger paw preferences were perceived as more confident, affectionate, active, and friendly than those with weaker paw preferences. Results suggest that motor laterality in the cat is strongly related to temperament and that the presence or absence of lateralization has greater implications for the expression of emotion in this species than the direction of the lateralized bias. (PsycINFO Database Record

  20. Day and night trophic variations of dominant fish species in a lagoon influenced by freshwater seeps.

    Science.gov (United States)

    Arceo-Carranza, D; Vega-Cendejas, M E; Hernández de Santillana, M

    2013-01-01

    The aim of this study was to determine the trophic structure and nycthemeral variations in the diet of dominant fish species (Ariopsis felis, Bairdiella chrysoura, Micropogonias undulatus, Eucinostomus gula, Eucinostomus argenteus, Lagodon rhomboides and Sphoeroides testudineus) in Celestun Lagoon, a biosphere reserve located in the southern Gulf of Mexico, and influenced by freshwater seeps. A total of 1473 stomachs were analysed and nine trophic groups were recorded. Bray-Curtis analyses with analyses of similarity (ANOSIM) statistical tests were used to determine two groups of feeding guilds: zoobenthivores and omnivores, with significant differences between time and habitat. The relationships between fish feeding habits, size class and environmental variables were investigated using canonical correspondence analysis (CCA). Most of the species showed a low niche breadth with high specialization towards amphipod consumption, with the exception of L. rhomboides (0·60), which indicated generalist feeding. This study in a protected area is an important source of information for drawing up conservation policies in relation to the management of aquatic resources, and will aid in the establishment of priority areas for conservation.

  1. Fecal endocrine profiles and ejaculate traits in black-footed cats (Felis nigripes) and sand cats (Felis margarita).

    Science.gov (United States)

    Herrick, J R; Bond, J B; Campbell, M; Levens, G; Moore, T; Benson, K; D'Agostino, J; West, G; Okeson, D M; Coke, R; Portacio, S C; Leiske, K; Kreider, C; Polumbo, P J; Swanson, W F

    2010-01-15

    Information regarding the reproductive biology of black-footed cats (BFC) and sand cats (SC) is extremely limited. Our objectives were to: (1) validate fecal hormone analysis (estrogens, E; progestagens, P; androgens, T) for noninvasive monitoring of gonadal activity; (2) characterize estrous cyclicity, ovulatory mechanisms, gestation, and seasonality; and (3) evaluate male reproductive activity via fecal androgen metabolites and ejaculate traits. In both species, the estrous cycle averaged 11-12 days. In BFC (n=8), estrus lasted 2.2+/-0.2 days with peak concentrations of E (2962.8+/-166.3 ng/g feces) increasing 2.7-fold above basal concentrations. In SC (n=6), peak concentrations of E (1669.9+/-83.5 ng/g feces) during estrus (2.9+/-0.2 days) were 4.0-fold higher than basal concentrations. Nonpregnant luteal phases occurred in 26.5% (26 of 98) of BFC estrous cycles, but were not observed in SC (0 of 109 cycles). In both species, P concentrations during pregnancy were elevated (32.3+/-3.0 microg/g feces BFC; 8.5+/-0.7 microg/g feces SC) approximately 10-fold above basal concentrations. Fecal T concentrations in males averaged 3.1+/-0.1 microg/g feces in BFC and 2.3+/-0.0 microg/g feces in SC. Following electroejaculation, 200 to 250 microl of semen was collected containing 29.9 (BFC) to 36.5 (SC)x10(6) spermatozoa with 40.4 (SC) to 46.8 (BFC)% normal morphology. All females exhibited estrous cycles during the study and spermatozoa were recovered from all males on every collection attempt, suggesting poor reproductive success in these species may not be due to physiological infertility.

  2. EVALUACIÓN DEL CRECIMIENTO DE JUVENILES DE PEZ BLANCO (Menidia estor) ALIMENTADOS CON DIETAS A BASE DE PROTEÍNA HIDROLIZADA DE BAGRE ARMADO (Pterygoplichthys disjunctivus).

    OpenAIRE

    AVALOS SÁNCHEZ, ANA MAURICIA

    2012-01-01

    El pez blanco Menidia estor (Jordan 1879) del Lago de Pátzcuaro, Michoacán, es una especie nativa y endémica del altiplano mexicano, perteneciente a la familia Atherinopsidae. Por su valor económico, cultural y nutricional es la especie de mayor importancia en la región, que por muchos años ha sido la base de la subsistencia del pueblo Purépecha. Debido a la intensa explotación y deterioro de su hábitat se le considera como una especie amenazada (Marez y Morales 2003), razón po...

  3. Spatial variability of primary organic sources regulates ichthyofauna distribution despite seasonal influence in Terminos lagoon and continental shelf of Campeche, Mexico

    Science.gov (United States)

    Romo Rios, J. A.; Aguíñiga-García, S.; Sanchez, A.; Zetina-Rejón, M.; Arreguín-Sánchez, F.; Tripp-Valdéz, A.; Galeana-Cortazár, A.

    2013-05-01

    Human activities have strong impacts on coastal ecosystems functioning through their effect on primary organic sources distributions and resulting biodiversity. Hence, it appears to be of utmost importance to quantify contribution of primary producers to sediment organic matter (SOM) spatial variability and its associated ichthyofauna. The Terminos lagoon (Gulf of Mexico) is a tropical estuary severely impacted by human activities even though of primary concern for its biodiversity, its habitats, and its resource supply. Stable isotope data (d13C, d15N) from mangrove, seaweed, seagrass, phytoplankton, ichthyofauna and SOM were sampled in four zones of the lagoon and the continental shelf through windy (November to February), dry (March to June) and rainy (July to October) seasons. Stable Isotope Analysis in R (SIAR) mixing model were used to determine relative contributions of the autotrophic sources to the ichthyofauna and SOM. Analysis of variance of ichthyofauna isotopic values showed significant differences (P < 0.001) in the four zones of lagoon despite the variability introduced by the windy, dry and rainy seasons. In lagoons rivers discharge zone, the mangrove contribution to ichthyofauna was 40% and 84% to SOM. Alternative use of habitat by ichthyofauna was evidenced since in the deep area of the lagoon (4 m), the contribution of mangrove to fish is 50%, and meanwhile contribution to SOM is only 77%. Although phytoplankton (43%) and seaweed (41%) contributions to the adjacent continental shelf ichthyofauna were the main organic sources, there was 37% mangrove contribution to SOM, demonstrating conspicuous terrigenous influence from lagoon ecosystem. Our results point toward organic sources spatial variations that regulate fish distribution. In Terminos lagoon, significant correlation (p-value = 0.2141 and r=0.79) of Ariopsis felis and Sphoeroides testudineus abundances and seaweed and seagrasses contributions (30-35%) during both dry and rainy seasons

  4. Biología de la gestación en la gata doméstica (Felis catus Biology of pregnancy in the domestic cat (Felis catus

    Directory of Open Access Journals (Sweden)

    A.E SANCHEZ

    2002-01-01

    Full Text Available La gata es una hembra poliéstrica estacional en la cual el estímulo coital desencadena la liberación de LH y la ovulación, fenómenos que ocurren dentro de las primeras 50 horas postcoito. La fecundación de los ovocitos ocurre en el oviducto dentro de 30 horas post-ovulación. El transporte embrionario en oviducto toma alrededor de 132 horas y al momento de ingresar al útero los embriones se encuentran al estado de mórula compacta. A continuación los blastocistos migran entre los cuernos uterinos por aproximadamente 80 horas, hasta producirse la implantación 12 a 13 días post-coito. Durante la etapa preimplantacional existe un aumento significativo de la progesterona sérica así como de los receptores luteales a LH. A partir de la segunda mitad de la gestación, decae la producción de progesterona y aumenta la secreción de prolactina, postulándose que esta última sería el principal agente luteotrófico en la gata. También durante la segunda mitad de la gestación aumenta la secreción de relaxina. La producción y rol de la progesterona en la gestación tardía es un tema controversial. Se ha demostrado que la placenta felina posee actividad esteroidogénica y capacidad de sintetizar progesterona, lo cual sugiere que estaría relacionada con el soporte hormonal de la preñezThe domestic cat can be defined as a seasonal poliestrous female. During mating the physical stimulus produce the release of LH and ovulation, which occur during the first 50 hours post mating. The fertilization of oocytes takes place inside the oviduct during the 30 hours post ovulation. The embryos transport on the oviduct takes about 132 hours and when embryos reach the uterus they are already a compacted morulae. After this, the blastocyst migrates to both uterine horns for a period of 80 hours aproximately until implantation ocurrs 12 to 13 days after mating. During the preimplantation period, there is a significant increase in blood progesterone and also in LH lutheal receptors. From the second half of gestation, the production of progesterone decrease and prolactin secretion increase. Thus it is thought that the later would be the main luteotrophic agent in the cat. Also during the second half of gestation the secretion of relaxin increase. The production and function of progesterone during late gestation is controversial. It has been shown that placenta of cats has a steroidogenic function and that it is also to produce progesterone

  5. Esporotricose do gato doméstico (Felis catus: transmissão humana Sporothricosis of the domestic cat (Felis catus: human transmission

    Directory of Open Access Journals (Sweden)

    Silvio Alencar Marques

    1993-08-01

    Full Text Available No presente trabalho relata-se caso de paciente, funcionário de hospital veterinário, infectado através de arranhadura de gato doméstico portador de esporotricose. Inquérito domiciliar junto aos proprietários do animal fonte de infecção, revelou dois outros casos presuntivos de esporotricose humana transmitida por gatos, e confirmou o diagnóstico, por cultivo do Sporotrix schenckii, em 3 gatos domésticos adicionais. A esporotricose felina caracteriza-se por lesões cutâneas ulceradas e tendência à disseminação sistêmica e evolução fatal. A transmissão intra e inter-espécie é facilitada pela exuberância de fungos nas lesões cutâneas de felinos infectados.A case of sporothricosis transmitted by cat to a veterinarian hospital employee is reported. Inquiry at domiciliar area of the cat's owner revelled two other presumable cases of human sporothricosis transmitted by cats, and confirmed the diagnosis (by culture of Sporothrix schenckii of disease in three other domestic cats. Feline sporothricosis is characterized by ulcerative, cutaneous lesions and systemic dissemination, which invariably cause animal's death. The transmission of sporothricosis to other animals and humans is enhanced by the great amount of fungus present in cat's lesions.

  6. Isolation and genetic characterization of Toxoplasma gondii from black bears (Ursus americanus), bobcats (Felis rufus), and feral cats (Felis catus) from Pennsylvania

    Science.gov (United States)

    Toxoplasma gondii infects virtually all warm-blooded hosts worldwide. Recently, attention has been focused on the genetic diversity of the parasite to explain its pathogenicity in different hosts. It has been hypothesized that interaction between feral and domestic cycles of T. gondii may increase u...

  7. Caracterización de la comunidad bacteriana aerobia en sanguijuelas (Hirudinea), Haementeria sp. (Glossiphoniidae) y Oxytychus ornatus (Macrobdellidae) de El Bagre, Antioquia = Characterization of the aerobic bacterial community in leeches Haementeria sp (Hirudinea: Glossiphoniidae) y Oxytychus ornatus (Hirudinea: Macrobdellidae) de El Bagre, Antioquia

    OpenAIRE

    López Isaza, Laura Patricia; Velásquez Trujillo, Luz Elena; Gómez Carmona, Catalina; Ramírez Pérez, Auxilio

    2012-01-01

    Se ha incrementado el uso terapéutico de las sanguijuelas y se ha demostrado que ellas pueden inocular bacterias causantes de infecciones en el 20% de los pacientes. El hallazgo en Antioquia de dos especies de sanguijuelas promisorias para hirudoterapia motivó este estudio para identificar las bacterias en la superficie, la probóscide y el intestino de estos anélidos y evaluar su sensibilidad a antibióticos. Las sanguijuelas estudiadas se identificaron como Haementeria sp., y Oxytychus ornatu...

  8. Caracterización de la comunidad bacteriana aerobia en sanguijuelas (Hirudinea, Haementeria sp. (Glossiphoniidae y Oxytychus ornatus (Macrobdellidae de El Bagre, Antioquia = Characterization of the aerobic bacterial community in leeches Haementeria sp (Hirudinea: Glossiphoniidae y Oxytychus ornatus (Hirudinea: Macrobdellidae de El Bagre, Antioquia

    Directory of Open Access Journals (Sweden)

    López Isaza, Laura Patricia

    2012-01-01

    Full Text Available Se ha incrementado el uso terapéutico de las sanguijuelas y se ha demostrado que ellas pueden inocular bacterias causantes de infecciones en el 20% de los pacientes. El hallazgo en Antioquia de dos especies de sanguijuelas promisorias para hirudoterapia motivó este estudio para identificar las bacterias en la superficie, la probóscide y el intestino de estos anélidos y evaluar su sensibilidad a antibióticos. Las sanguijuelas estudiadas se identificaron como Haementeria sp., y Oxytychus ornatus. Muestras de la superficie, boca e intestino de ambos anélidos se inocularon en agar sangre, agar sangre con ampicilina y agar eosina azul de metileno. La identificación de las bacterias y su sensibilidad a antibióticos se evaluaron por el método automatizado Vitek (Biomerieux®. Se hicieron en total 26 aislamientos pertenecientes a 12 especies de nueve géneros. Enterobacter cloacae fue la especie más frecuente en ambos anélidos. Las bacterias fueron sensibles a los antibióticos comúnmente empleados en las infecciones causadas por este tipo de microorganismos. Se sugiere hacer profilaxis con antibióticos en los pacientes que reciban terapia con los anélidos investigados e implementar los protocolos estandarizados para el lavado de los animales antes de su uso y para el aseo de los acuarios en donde se los mantenga.

  9. Estudo bioquímico e fisiológico sobre os bagres marinhos do Brasil: I. Sobre padrão eletroforético do plasma em gel de poliacrilamida dos bagres da região estuarino lagunar de Cananéia

    Directory of Open Access Journals (Sweden)

    Phan Van Ngan

    1980-12-01

    Full Text Available The possibility of the use of electropherogrames of plasma proteins in polyacrylamide slab gel in the study of populations of marine cat-fishes was examined. A total of 176 samples of plasma from four species. Sciadeichtys luniscutis. Arius spixii, Genidens genidens and Netuma barbas was used. Inter-specific as well as intra-specific differences were found and the locus E of the plasma of all the four species showed a typical dialelic variation. The locus is recommended as one of alternatives which deserve due consideration in the planing of studies of populations of these species by means of biochemical methods.

  10. Coat genetic markers of the domestic cat Felis catus (Felidae from southwestern Colombia

    Directory of Open Access Journals (Sweden)

    Mauricio Peñuela A

    2016-05-01

    Full Text Available Objectives. Establish the genetic profiles of cats from 12 neighboring municipalities in southwestern Colombia, in a town course from Pereira-Popayán. Estimate the degree of diversity, genetic structure, and quantify gene flow. Materials and methods. Were inventoried the phenotypic markers present in the pigmentation and structure of the coat of 1482 cats of the municipalities surveyed. Based on these phenotypic frequencies, allele frequencies, heterozygosity, Hardy-Weinberg equilibrium, F statistics and Nei genetic distances were calculated. A comparison was also made between genetic and geographic distance matrices to determine if there was a significant association between the two. Results. With the genetic profiles of the populations we estimated the degree of diversity. We found the populations in equilibrium for the S autosomal locus and for the O sex-linked locus. We found a low level genetic structure, and it was determined that there was no significant correlation between the genetic and geographic distance matrices among populations. Conclusions: These findings can be explained on the basis of the processes of human displacement for this region, due to the fact that the establishment of feline populations in these municipalities originated during the same historical period. Identical genetic profiles are shared as a result of colonization events, and due to possible continued migration among these populations.

  11. An STR forensic typing system for genetic individualization of domestic cat (Felis catus) samples.

    Science.gov (United States)

    Menotti-Raymond, Marilyn A; David, Victor A; Wachter, Leslie L; Butler, John M; O'Brien, Stephen J

    2005-09-01

    A forensic genotyping panel of 11 tetranucleotide STR loci from the domestic cat was characterized and evaluated for genetic individualization of cat tissues. We first examined 49 candidate STR loci and their frequency assessment in domestic cat populations. The STR loci (3-4 base pair repeat motifs), mapped in the cat genome relative to 579 coding loci and 255 STR loci, are well distributed across the 18 feline autosomes. All loci exhibit Mendelian inheritance in a multi-generation pedigree. Eleven loci that were unlinked and were highly heterozygous in cat breeds were selected for a forensic panel. Heterozygosity values obtained for the independent loci, ranged from 0.60-0.82, while the average cat breed heterozygosity obtained for the 11 locus panel was 0.71 (range of 0.57-0.83). A small sample set of outbred domestic cats displayed a heterozygosity of 0.86 for the 11 locus panel. The power of discrimination of the panel is moderate to high in the cat breeds examined, with an average P(m) of 3.7E-06. The panel shows good potential for genetic individualization within outbred domestic cats with a P(m) of 5.31E-08. A multiplex protocol, designed for the co-amplification of the 11 loci and a gender-identifying locus, is species specific and robust, generating a product profile with as little as 0.125 nanograms of genomic DNA.

  12. Maxillary incisor root resorption after rapid palatal expansion in Felis catus.

    Science.gov (United States)

    Vardimon, Alexander D; Levy, Thierry; Weinreb, Miron

    2005-02-01

    Root resorption after rapid palatal expansion (RPE) treatment was found in anchored teeth but has not been studied on non-anchored incisors. This study evaluated root resorption, root tipping, and root proximity of maxillary incisors after RPE treatment. Fourteen cats were divided into treated (n = 10) and untreated (n = 4) groups. The RPE treatment consisted of active, retention, and relapse phases, lasting 25, 60 and 60 d, respectively. Standardized occlusal radiographs were taken to measure tipping and root proximity before and after each treatment phase. Maxillary incisors were analysed histologically by fluorescent microscopy for root resorption. Data was analysed statistically with anova with repeated measures, t-test and Pearson's coefficient of correlation. Root resorption was confined to the first incisors and was 750-fold greater in the treated vs. the control group. Root tipping and root proximity were significantly greater (2.5- and 17-fold, respectively) in the first than in the second maxillary incisor and highly correlated with root resorption (r = -0.927 and 0.723, respectively). This suggests a cause (tipping and root proximity) and effect (root resorption) relationship. Data suggest that first maxillary incisor susceptibility to root resorption during RPE is associated with severe tipping and root proximity.

  13. Estudo anatômico do trajeto do canal mandibular em felinos (Felis catus domesticus

    Directory of Open Access Journals (Sweden)

    S.C. Cotrim

    2015-12-01

    Full Text Available Objetivou-se descrever, por meio de tomografia computadorizada, o trajeto do canal mandibular (CM em 20 gatos sem raça definida, com ausência de alterações na cavidade oral, provenientes do Centro de Controle de Zoonoses do Distrito Federal. Foram realizados cortes tomográficos com 2mm de espessura, acompanhando todo o trajeto do CM, tendo como referência a região do forame mandibular, as raízes distais e mesiais dos dentes pré-molares e molares e o forame mentoniano, obtendo-se medidas desde o CM até as faces vestibular, lingual, ventral e alveolar (profundidade do corpo da mandíbula, bem como seu diâmetro. Pôde constatar que o CM manteve-se no aspecto lingual do corpo da mandíbula desde o forame mandibular até a raiz mesial do 1º pré-molar, onde se deslocou para a face vestibular, emergindo no forame mentoniano. Com relação à profundidade, seu trajeto sofreu declive a partir do forame mandibular até a região da raiz mesial do 1º molar, onde alcançou seu ponto mais profundo para prosseguir em suave ascensão até o forame mentoniano. Os dados apresentados contribuem para o estudo anatômico da mandíbula de gatos, bem como auxiliam no melhor planejamento e execução de procedimentos cirúrgicos na mandíbula dessa espécie.

  14. Evaluation of cats (Felis catus) as possible asymptomatic carriers of dermatophytes in extreme south of Brazil

    Institute of Scientific and Technical Information of China (English)

    Tony Silveira; Renata de Faria; Mariana Remio; Camila Graeff; Fabiana Poetsch; Guilherme Azevedo; Juliane Guimares; Rafaela Bellora; Tassiane Moraes; Pedro Quevedo

    2015-01-01

    Objective: To investigate the presence of Microsporum canis in pelage of asymptomatic cats for dermatophytosis, in south region of Rio Grande do Sul, Brazil, and evaluate its importance in epidemiology of dermatophytosis in the study area. Methods: A total of 60 domestic cats were evaluated for the presence of Microsporum canis. The animals were divided into three groups of 20 felines. Each group consisted of exclusively domiciled, semi-domiciled and rural animals. Samples were collected following the carpet-square technique. The microorganisms were cultivated under laminar flow in mycosel agar and grown in a greenhouse. Results: All the cats of the three groups analysed had negative cultures for dermatophytes. In 85% of the dishes, there was a growth of environmental saprophytic fungi such as Aspergillus sp., Fusarium sp. and Penicillium sp. Conclusions: Thus, asymptomatic cats for dermatophytes did not show importance in the transmission and maintenance of the disease in southern of Rio Grande do Sul, Brazil.

  15. On Filaria-specimens from the right ventricle of the heart of Felis onca

    NARCIS (Netherlands)

    Horst, R.

    1889-01-01

    Some time ago Mr. J. H. Spitzly kindly forwarded to our Museum a few bottles with parasitic worms, collected by him in Surinam. Among them there was a bottle, containing some Nematodes found in the heart of a young female Jaguar, that was killed by a hunter. In reference to these parasites Mr. Spitz

  16. Growth of a male Caracal kitten felis Caracal in the Mountain Zebra National Park

    Directory of Open Access Journals (Sweden)

    J. H Grobler

    1982-11-01

    Full Text Available Three caracal (lynx Fells caracal kittens, two females and a male were received from the Fish River area some 50 km north of Cradock, Republic of South Africa, on the 20th March 1980. These were found at the base of a dense Rhus erosa bush on the side of a hill and estimated at 14 days old based on the findings of Cade (1968 Int. Zoo Yb. 45 and Kralik (1967 Int. Zoo Yb. 132.

  17. ENTEROPARÁSITOS EN PERROS (Canis familiaris) Y GATOS (Felis catus) DE LA PROVINCIA DE PUNO

    OpenAIRE

    VILCA DE DIAZ, FELICIANA; Universidad Nacional del Altiplano; MELO ANCCASI, MAXIMO; Universidad Nacional del Altiplano

    2013-01-01

    ResumenLas mascotas que conviven con el hombre, el  perro y el gato ocupan un lugar muy especial, como amigos fieles, especialmente de los niños; sin embargo esta relación se interrumpe por la presencia de parásitos ciclozoonóticos, ocasionando múltiples enfermedades, incluso en algunos casos podrían ocasionar  la muerte, con repercusiones socioeconómicas de impacto. Por ello la necesidad de realizar esta investigación para determinar la prevalencia de entero parásitos  en estas especies, eva...

  18. Population density estimation of the European wildcat (Felis silvestris silvestris in Sicily using camera trapping

    Directory of Open Access Journals (Sweden)

    Stefano Anile

    2012-07-01

    Full Text Available The wildcat is an elusive species that is threatened with extinction in many areas of its European distribution. In Sicily the wildcat lives in a wide range of habitats; this study was done on Mount Etna. A previous camera trap monitoring was conducted in 2006 (pilot study and 2007 (first estimation of wildcat population size using camera trapping with capture-recapture analyses in the same study area. In 2009 digital camera traps in pair were used at each station with the aim of obtaining photographs of the wildcat. Experience and data collected from previous studies were used to develop a protocol to estimate the density of the wildcat’s population using capture–recapture analyses and the coat-colour and markings system to recognize individuals. Two trap-lines adjacent to each other were run in two consecutive data collection periods. Camera traps worked together for 1080 trap-days and we obtained 42 pictures of wildcats from 32 events of photographic capture, from which 10 individuals ( excluding four kittens were determined. The history capture of each individual was constructed and the software CAPTURE was used to generate an estimation of the population density (0.22 to 0.44 wildcat/100 ha for our study area using two different approaches for the calculation of the effective area sampled. The wildcat’s population density on Mount Etna is higher than those found throughout Europe, and is favoured by the habitat structure, prey availability, Mediterranean climate and the protection status provided by the park.

  19. Função testicular em gatos domésticos (Felis catus)

    OpenAIRE

    Meyer, Katlyn Barp

    2013-01-01

    Resumo: O presente trabalho teve como objetivo avaliar a esteroidogênese e a espermatogênese em relação à qualidade espermática em machos de gatos domésticos de forma a responder questões sobre a sazonalidade reprodutiva e o fenômeno da teratospermia em felinos. Foram realizadas análises da morfologia e de parâmetros físicos de espermatozóides obtidos da cauda do epidídimo, mensuração das concentrações sérica de testosterona (T) e intratesticulares de T e 17?-estradiol (E2) e análise da ativi...

  20. [Architectural structure of the pterygoidian plane of the masticatory musculature in the cat (Felis catus L.)].

    Science.gov (United States)

    Lautrou, A; Laison, F

    1975-09-01

    The architectural analysis of the ptérygoidal plane of the cat identified the two pterygoid muscles. The medial pterygoid showed an orbital and an angular part inserted respectively on the internal face of the mandibule and on the maeto-angular ligament. The lateral pterygoid consisted of only one pterygoid bundle, involved in lateral movements and adjustments of the jaw condyle.

  1. Serotyping of Toxoplasma gondii in Cats (Felis domesticus) Reveals Predominance of Type II Infections in Germany

    Science.gov (United States)

    Background: Cats are definitive hosts of Toxoplasma gondii and play an essential role in the epidemiology of this parasite. The study aims at clarifying whether cats are able to develop specific antibodies against different clonal types of T. gondii and to determine by serotyping the T. gondii clona...

  2. Seroprevalence of Toxoplasma gondii in Cats (Felis catus, Linnaeus 1758 Living in San Carlos (Chile

    Directory of Open Access Journals (Sweden)

    Ignacio Eduardo Troncoso Toro

    2015-05-01

    Full Text Available There are few studies about seroprevalence of toxoplasmosis in Chile; therefore, this article aims to determine seroprevalence in cats in the district of San Carlos, by ELISA Immuno- Comb® serological technique, and, at the same time, to examine association with variables of sex, age, diet, and habitat. To the effect, 60 cats over 2 months old were randomly sampled. Sera were analyzed using the ELISA ImmunoComb® Biogal Toxo & Chlamydia test kit, which detects specific immunoglobulin G-type antibodies against Toxoplasma gondii with a sensitivity of 92.3% and a specificity of 100%. The study evidenced that 29 individuals were positive (48.3% seroprevalence; when broken down by gender this corresponded to 9 males and 20 females (39.1% and 54%, respectively. By age, seropositivity was higher in the “Adult” group (76.7%, followed by groups “Over 7 years” (50% and “Young” (25%. With respect to diet, higher seropositivity was obtained in animals fed on mixed diet, as opposed to commercial diet (60% vs. 47.2%. By variable habitat, 16 indoor and 13 outdoor cats were positive (45.7% and 52%, showing statistically significant difference only for the variable age (p < 0.05. Finally, through relating age with seropositivity, a negative correlation was evidenced (r = –0.3, indicating that older individuals had lower seroprevalence. The results show the presence of antibodies against Toxoplasma gondii in domestic cats.

  3. Detection of antibodies against Leishmania infantum in cats (Felis catus from the State of Pernambuco, Brazil

    Directory of Open Access Journals (Sweden)

    Rita de Cássia Nascimento Silva

    2014-01-01

    Full Text Available Introduction: Little information is available concerning infection by Leishmania infantum in cats. Therefore, the aim of this study was to perform a serological study in domestic cats. Methods: Serum samples (n=153 obtained from animals living in the Cities of Recife and Petrolina, State of Pernambuco, Brazil, were tested by ELISA/S7® (Biogene. Results: Anti-L. infantum antibodies were detected in 3.9% (6/153 of the cats. All seroreagent animals were from Petrolina. Conclusions: These results serve as an important alert, and future studies are needed to better understand the possible role of cats in the epidemiology of visceral leishmaniasis (VL in this area.

  4. Distribuição, abundância relativa e movimentos sazonais de bagres marinhos (Siluriformes, Ariidae no estuário da Lagoa dos Patos (RS, Brasil

    Directory of Open Access Journals (Sweden)

    Francisco Gerson Araújo

    1988-01-01

    Full Text Available Three ariid fish, Netuma barba, Netuma planifrons and Genidens genidens, use the estuary of the Patos Lagoon (32ºS, Rio Grande do Sul, Brazil, as a nursery and feeding ground in various stages of their life history. A comparative study was made of the temporal and spatial distribution and relative abundance. Seasonal movements were interpreted on the basis of distribution and abundance of the young of these coexisting sea catfishe. Monthly otter trawl samples were made between November, 1979 and April, 1983. Temperature, salinities and dissolved oxygen contents were taken at each of the 674 sample stations. All three species were caught in ample temperature (12,1 to 26,3ºC, salinity (0,3 to 35,5‰ and dissolved oxygen (4,7 to 11,5 mg/l ranges. However, they were more abundant in stations of low temperature and in salinity with highly dissolved oxygen content. N. barba was the most abundant catfish caught in the estuary, it constituted 81,9% in number and 81,4% in weight of ali ariid trawl catches during the study. N. barba occurred throughout the estuary, from the adjacent coastal region to the upper reaches of the Patos Lagoon estuary near the liminetic zone. N. planifrons occurred from adjacent coastal regions to low saline parts of the Patos Lagoon estuary, whereas G. genidens were distributed mainly in low saline and freshwater parts of the lagoon. Young-of-the-year N. barba aggregated near of the entrance of the lagoon in fall and winter, then moved up to intermediary parts of the estuary in spring, and they went to the upper part of the estuary in summer. Yearlings N. barba moved into the estuary in common with young-of-the-year but did not reach the coastal adjacent area. Young-of-the-year and yearling N. planifrons moved in to the estuary as the same pattern as N. barba but they became scarce during the late summer and early fall. G. genidens were scarcely captured in the estuary throughout the year. The spatial and temporal segregations among Netuma and Genidens were evident. A hypothetical model for the life history of N. barba is suggested.

  5. Acute Toxicity Of Euphorbia Royleana Boiss (Euphorbiaceae latex on freshwater catfish, Heteropneustes Fossilis (Siluriformes, Heteropneustidae Toxicidad aguda del látex de Euphorbia royleana Boiss (Euphorbiaceae en el bagre de agua dulce, Heteropneutes fossilis (Siluriformes, Heteropneustidae

    Directory of Open Access Journals (Sweden)

    Prasad ManiRam

    2010-06-01

    Full Text Available An acute toxicity test was performed by using a four-day static renewal test to determine the LC50 value of aqueous extract of Euphorbia royleana latex for the freshwater fsh, Heteropneustes fossilis. The LC50 values, their upper and lower confdence limits and slope functions were calculated. The LC50 values for aqueous extract of Euphorbia royleana latex at various exposure periods were 7.758 mg/L for 24 h, 5.847 mg/L for 48 h, 4.474 mg/L for 72 h and 3.090 mg/L for 96 h. The regression coeffcient showed that there was signifcant negative correlation between exposure time and different LC values. Hence, it is concluded that the concentration to produce toxicity of latex of Euphorbia royleana is comparable and close to the concentration to produce toxicity of synthetic organophosphates pesticides for the fsh H. fossilis. Therefore, adequate precautions must be taken when Euphorbia royleana latex is being used near fsh- inhabited areas.La prueba de la toxicidad aguda fue realizada utilizando un test estático con renovación, de cuatro días de duración, para determinar el valor de la CL50 de un extracto acuoso del látex de Euphorbia royleana, en el pez de agua dulce Heteropneustes fossilis. Se calcularon el valor de la CL50, los límites de confanza máximo y mínimo y la pendiente. Los valores de la CL50 para el extracto acuoso del látex en varios períodos de exposición fueron 7,758 mg/L para 24 h, 5,847 mg/L para 48 h, 4,474 mg/L para 72 h y 3,090 mg/L para 96 h. El coefciente de regresión mostró una correlación negativa signifcativa entre el tiempo de exposición y diferentes valores de la CL50. Se concluye que la concentración del látex de E. royleana que produce toxicidad es comparable y cercana a la de los plaguicidas sintéticos organofosforados para el pez H. fossilis. Por lo tanto, se deben tomar precauciones adecuadas cuando el látex de E. royleana es utilizado cerca de áreas donde habita el pez H. fossilis.

  6. Revalidação de Cathorops arenatus e Cathorops agassizii (Siluriformes, Ariidae, bagres marinhos das regiões norte e nordeste da América do Sul

    Directory of Open Access Journals (Sweden)

    Alexandre P. Marceniuk

    2007-12-01

    Full Text Available As espécies nominais das regiões norte e nordeste da América do Sul, reconhecidas na literatura como sinônimos júnior de Cathorops spixii (Agassiz, 1829 têm seu status específico revisto. Os tipos de Arius arenatus Valenciennes, 1840, Arius fissus Valenciennes, 1840, Tachisurus agassizii Eigenmann & Eigenmann, 1888 e Arius pleurops Boulenger, 1897 foram examinados. Cathorops arenatus e Cathorops agassizii são espécies válidas, Cathorops fissus é sinônimo júnior de Cathorops arenatus e Cathorops pleurops sinônimo júnior de Cathorops agassizii. Os caracteres morfométricos tradicionalmente usados em descrições e chaves de identificação para espécies da família Ariidae são redefinidos e novos caracteres são utilizados.Nominal species from north and northeast South America, traditionally recognized in the literature as synonymous of Cathorops spixii (Agassiz, 1829, have their specific status redefined. Type specimens of Arius arenatus Valenciennes, 1840, Arius fissus Valenciennes, 1840, Tachisurus agassizii Eigenmann & Eigenmann, 1888 and Arius pleurops Boulenger, 1897 were examined. Cathorops arenatus and Cathorops agassizii are considered valid, Cathorops fissus is a junior synonym of Cathorops arenatus and Cathorops pleurops a junior synonym of Cathorops agassizii. The morphometric characters traditionally used in descriptions and keys in the family Ariidae are redefined and new characters are used.

  7. Fontes não-convencionais de proteína na primeira alimentação do bagre sul americano (Rhamdia quelen Unconventional protein sources in the first feeding of South American catfish (Rhamdia quelen

    Directory of Open Access Journals (Sweden)

    David Roque Hernández

    2009-06-01

    Full Text Available O presente estudo teve como objetivo avaliar diferentes fontes de proteína na primeira alimentação de jundiá (Rhamdia quelen sob condições de laboratório. Foram testadas três rações balanceadas secas: ovo de peixe (TA, hidrolisado de carcaça do peixe (TB e fígado de frango cru (TC, na quais foram analisadas, pelo crescimento, a biomassa final produzida e a sobrevivência das larvas. O ensaio teve uma duração de 20 dias, e as rações foram fornecidas ad libitum. As larvas alimentadas com ovo de peixe apresentaram os melhores resultados com peso médio individual de 340mg, sobrevivência de 57% e biomassa final de 1.350mg, diferindo significativamente dos demais tratamentos. Os resultados indicaram que a formulação de rações balanceadas com ovos de peixes fornecida na primeira alimentação de larvas de jundiá pode tornar-se uma alternativa promissora na larvicultura dessa espécie.This study aimed to evaluate different proteins sources in first feeding of jundiá (Rhamdia quelen under laboratory conditions. Three balanced dry rations were tested, fish roe (TA, biological fish silage (TB and crude chicken liver (TC evaluating specific growth rate, final biomass produced and survival rate. The experiment was performed in 20 days, and all the rations were provided ad libitum. Larvae fed with fish roe presented better results, with 340mg of individual mean weight, 57% of survival rate and 1.350mg of final biomass, differing significantly with the other treatments. The results show that the inclusion of fish roe in balanced rations offered at the first feeding of jundiá larvae may become a promising alternative for the hatchering of the species.

  8. Five-week dietary exposure to dry diets alters the faecal bacterial populations in the domestic cat (Felis catus).

    Science.gov (United States)

    Bermingham, Emma N; Kittelmann, Sandra; Henderson, Gemma; Young, Wayne; Roy, Nicole C; Thomas, David G

    2011-10-01

    The effects of wet (canned) or dry (kibbled) diets on faecal bacterial populations in the cat were investigated in eight domestic short-haired cats (four males and four females; averaging 6 years of age and 3.4 kg) in a nested design. The cats were fed ad libitum a commercially available wet diet (moisture 82.0 %, crude protein 51.7 %, fat 28.9 %, carbohydrate (CHO) 8.9 % and ash 10.6 % DM) for 5 weeks. On the fifth week, individual feed intakes and faecal outputs were determined. Fresh faecal samples were collected twice daily, mixed for homogeneity, subsampled and stored at - 85 °C until analysis. The cats were then switched to a commercially available dry diet (moisture 8.5 %, crude protein 33.0 %, fat 11.0 %, CHO 49.4 % and ash 6.6 % DM) for 5 weeks, and fresh faeces were sampled as described previously. Energy intake tended to be higher in cats fed dry diets (P 0.05). Denaturing gradient gel electrophoresis (DGGE) of bacterial 16S rRNA genes amplified from DNA extracted from faeces was performed. The unweighted pair group method with arithmetic mean cluster analysis of bacterial community profiles using Pearson's correlation revealed diet-specific clustering when the same cats were fed on either a dry or a wet diet (dissimilarity between the groups, 88.6 %; P Pelomonas and Fusobacteriaceae were influenced by a short-term change in diet format. This suggests that 5-week dietary exposure is sufficient to alter gastrointestinal microflora.

  9. Propionate absorbed from the colon acts as gluconeogenic substrate in a strict carnivore, the domestic cat (Felis catus)

    DEFF Research Database (Denmark)

    Verbrugghe, A; Hesta, M; Daminet, S

    2012-01-01

    In six normal-weight and six obese cats, the metabolic effect of propionate absorbed from the colon was assessed. Two colonic infusions were tested in a crossover design with intervals of 4 weeks. The test solution contained 4 mmol sodium propionate per kg ideal body weight in a 0.2% NaCl solution...

  10. Different patterns of metabolic cryo-damage in domestic cat (Felis catus) and cheetah (Acinonyx jubatus) spermatozoa.

    Science.gov (United States)

    Terrell, Kimberly A; Wildt, David E; Anthony, Nicola M; Bavister, Barry D; Leibo, S P; Penfold, Linda M; Marker, Laurie L; Crosier, Adrienne E

    2012-04-01

    Felid spermatozoa are sensitive to cryopreservation-induced damage, but functional losses can be mitigated by post-thaw swim-up or density gradient processing methods that selectively recover motile or structurally-normal spermatozoa, respectively. Despite the importance of sperm energy production to achieving fertilization, there is little knowledge about the influence of cryopreservation or post-thaw processing on felid sperm metabolism. We conducted a comparative study of domestic cat and cheetah sperm metabolism after cryopreservation and post-thaw processing. We hypothesized that freezing/thawing impairs sperm metabolism and that swim-up, but not density gradient centrifugation, recovers metabolically-normal spermatozoa. Ejaculates were cryopreserved, thawed, and processed by swim-up, Accudenz gradient centrifugation, or conventional washing (representing the 'control'). Sperm glucose and pyruvate uptake, lactate production, motility, and acrosomal integrity were assessed. Mitochondrial membrane potential (MMP) was measured in cat spermatozoa. In both species, lactate production, motility, and acrosomal integrity were reduced in post-thaw, washed samples compared to freshly-collected ejaculates. Glucose uptake was minimal pre- and post-cryopreservation, whereas pyruvate uptake was similar between treatments due to high coefficients of variation. In the cat, swim-up, but not Accudenz processing, recovered spermatozoa with increased lactate production, pyruvate uptake, and motility compared to controls. Although confounded by differences in non-specific fluorescence among processing methods, MMP values within treatments were positively correlated to sperm motility and acrosomal integrity. Cheetah spermatozoa isolated by either selection method exhibited improved motility and/or acrosomal integrity, but remained metabolically compromised. Collectively, findings revealed a metabolically-robust subpopulation of cryopreserved cat, but not cheetah, spermatozoa, recovered by selecting for motility rather than morphology.

  11. Correlation between fetal age and ultrasonographic measurements during the second half of pregnancy in domestic cats (Felis catus).

    Science.gov (United States)

    Zambelli, Daniele; Castagnetti, Carolina; Belluzzi, Stefano; Paladini, Cosimo

    2004-11-01

    We ultrasonographically evaluated the prenatal development in cats, from Day 30 to the end of pregnancy, subjecting a group of pregnant cats (n = 8) to daily ultrasonographic examinations. The ultrasonographic images allowed us to measure the diameter of the fetal abdomen, the biparietal diameter of the fetal skull and the diameter of the fetal stomach. A correlation between these measurements and gestational age was found with a linear, parabolic and exponential regression analysis. From Days 38 to 43 after breeding we also performed fetal gender determination by evaluating the external genitalia. All queens successfully carried their pregnancies to term. This study compiled useful new data in order to clinically monitor the normal course of pregnancy in cats and to determine gestational age.

  12. Seasonal Changes in Testes Vascularisation in the Domestic Cat (Felis domesticus: Evaluation of Microvasculature, Angiogenic Activity, and Endothelial Cell Expression

    Directory of Open Access Journals (Sweden)

    Graça Alexandre-Pires

    2012-01-01

    Full Text Available Some male seasonal breeders undergo testicular growth and regression throughout the year. The objective of this study was to understand the effect of seasonality on: (i microvasculature of cat testes; (ii angiogenic activity in testicular tissue in vitro; and (iii testicular endothelial cells expression throughout the year. Testicular vascular areas increased in March and April, June and July, being the highest in November and December. Testes tissue differently stimulated in vitro angiogenic activity, according to seasonality, being more evident in February, and November and December. Even though CD143 expression was higher in December, smaller peaks were present in April and July. As changes in angiogenesis may play a role on testes vascular growth and regression during the breeding and non-breeding seasons, data suggest that testicular vascularisation in cats is increased in three photoperiod windows of time, November/December, March/April and June/July. This increase in testicular vascularisation might be related to higher seasonal sexual activity in cats, which is in agreement with the fact that most queens give birth at the beginning of the year, between May and July, and in September.

  13. Molecular detection of Leishmania sp. in cats (Felis catus) from Andradina Municipality, São Paulo State, Brazil.

    Science.gov (United States)

    Coelho, Willian Marinho Dourado; Richini-Pereira, Virgínia Bodelão; Langoni, Helio; Bresciani, Katia Denise Saraiva

    2011-03-10

    The aim of this work was to molecularly detect Leishmania species in 52 cats from Andradina Municipality, São Paulo State, Brazil. The direct parasitological test was performed by using imprints of poplited lymph node, bone marrow and spleen to verify amastigote forms of Leishmania spp. The samples that were positive parasitological tests were subjected to molecular analysis (PCR) and sequencing. Infection was detected for 5.76% (3/52) of the examined cats and two had presence of amastigote forms of Leishmania spp. in lymph nodes. Polymerase chain reaction (PCR) of kinetoplast minicircle DNA, indicated positive amplification for samples of spleen and lymph nodes and the sequencing resulted in 97% similarity with Leishmania (L.) chagasi. This study proved the occurrence of infection with Leishmania (L.) chagasi in felines from Andradina municipality, São Paulo State.

  14. Occurrence of Leishmania (Leishmania) chagasi in a domestic cat (Felis catus) in Andradina, São Paulo, Brazil: case report.

    Science.gov (United States)

    Coelho, Willian Marinho Dourado; Lima, Valéria Marçal Felix de; Amarante, Alessandro Francisco Talamini do; Langoni, Helio; Pereira, Virgínia Bodelão Richini; Abdelnour, Aziz; Bresciani, Katia Denise Saraiva

    2010-01-01

    This work describes natural infection by Leishmania in a domestic cat where amastigote forms of the parasite were observed in the popliteal lymph node imprint. Positive and negative serological reactions were observed by enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence assay (IFA), respectively. Polymerase chain reaction (PCR) revealed that the nucleotide sequence of the sample was identical to Leishmania (L.) chagasi. This is the first report of the disease in felines of the city of Andradina, SP, an area considered endemic for canine and human visceral leishmaniasis.

  15. Ocorrência do vírus da leucemia felina em Felis cattus em Belo Horizonte

    Directory of Open Access Journals (Sweden)

    F.M. Coelho

    2011-06-01

    Full Text Available Blood samples from 1,072 domestic cats of nine administrative regions of Belo Horizonte, MG, were collected and tested using PCR nested for the occurrence of feline leukemia virus (FeLV. Overall occurrence was 47.5% (507/1072 being North (68.1% and East (54.4% the most prevalent areas. Epidemiological data showed that FeLV infection was very common among examined cats and breed neither gender nor were predisposing factors for FeLV. The results suggest that the agglomeration of a large number of cats in the same environment can be an important factor for the increase in the rate of transmission of this retrovirus among domestic cats in the studied city.

  16. Expression profiles of relaxin family peptides and their receptors indicate their influence on spermatogenesis in the domestic cat (Felis catus).

    Science.gov (United States)

    Braun, B C; Müller, K; Jewgenow, K

    2015-07-01

    Disturbed spermatogenesis is a common problem in felines. Studying spermatogenesis in the domestic cat can improve the understanding of the biological background and help to counteract fertility problems in other feline species. Here, we analyzed 3 relaxin family peptides (relaxin, relaxin-3, and INSL3) and their receptors (RXFP1, RXFP2, and RXFP3) as potential spermatogenic factors involving their expression in the testis at different stages of its development. It may be concluded from its stage-dependent expression that relaxin, together with RXFP1, appears to be involved in the first stage of spermatogenesis, whereas relaxin-3 via binding to RXFP3 influences spermiogenesis. Furthermore, correlations were observed between relaxin, relaxin-3, RXFP1, RXFP2 and RXFP3 messenger RNA expression, and the relative numbers of haploid cells in testes. The peptide INSL3 was highly expressed at all testis development stages. Because of the low and stage-independent expression of its receptor RXFP2, an auto- and/or paracrine function of INSL3 in spermatogenesis seems unlikely. In the adult testis, messenger RNA expression of relaxin, RXFP1, and RXFP3 predominantly occurs in the tubular testis compartment, whereas INLS3 is mainly expressed in the interstitium.

  17. INDUCTION OF NITRIC OXIDE SYNTHASE AND ASSOCIATED TOXICITY IN LIVERS OF HARDHEAD CATFISH, ARIUS FELIS, FROM CONTROL AND EPIZOOTIC SITES

    Science.gov (United States)

    Earlier work with a live channel catfish (Ictalurus punctatus) pathogen, Edwardsiella ictaluri, demonstrated the induction of nitric oxide synthase (NOS) in the head kidney, paralleling enteric septicemia (Hawke et al. 1981; Schoor and Plumb 1994). However, another study exposing...

  18. L'areale italiano del gatto selvatico europeo (Felis silvestris silvestris: ancora un dilemma?

    Directory of Open Access Journals (Sweden)

    Bernardino Ragni

    2003-10-01

    Full Text Available Nel 1992 dall'Aspromonte l'area di distribuzione del gatto selvatico europeo si arrestava al parallelo UTM 4800000 Nord, su una linea corologica che andava da Piombino a Fabriano (Ragni et al. 1994. Nessuna spiegazione ecologica poteva giustificare i 450 chilometri di iato corologico che separava tale linea dal frammento di areale disgiunto delle Alpi Liguri; ad Est la stazione più prossima era ubicata nell'area del Cansiglio (Prealpi Venete. Dopo un decennio si presenta un aggiornamento sullo stato del "dilemma". Dal giugno 1992 al giugno 2003 sono stati raccolti 46 reperti (esemplari rinvenuti morti, catturati vivi o fotografati in natura in Toscana, Umbria e Marche, le regioni "tagliate" dal limite d'areale peninsulare. Uno solo dei reperti risulta rinvenuto a Nord di detto limite: "sui monti di Carpegna", in Provincia di Pesaro-Urbino all'altezza del parallelo UTM 4850000 Nord; si tratta di un maschio adulto, abbattuto illegalmente. La nuova stazione zoogeografica è separata dal limite del 1992 con un salto longitudinale di almeno 50 chilometri, così come i 12440 Kmq di Penisola Italiana compresi tra i due paralleli risultano completamente privi del felide. Analogamente a quello spaziale si osserva un salto temporale di 10 anni (giugno 1992-ottobre 2002 tra le due condizioni corologiche. Al contrario, i 13200 Kmq di penisola compresi tra il parallelo "storico" e quello posto 50 chilometri più a Sud, sono punteggiati da 35 reperti con distribuzione spaziale relativamente uniforme; così come la distribuzione delle stazioni nel tempo. Se si esclude il ritrovamento di Carpegna i limiti di areale definiti nel 1992 sono rimasti immutati: le nuove stazioni peninsulari confermano, al giugno 2003, la linea Piombino-Fabriano; le nuove stazioni Nord-orientali confermano, all'ottobre del 2002, l'area del Cansiglio; mentre, dopo il 1992, le stazioni della Liguria occidentale, che risalivano già al 1981, non hanno ricevuto alcuna conferma. In conclusione, dal punto di vista della dinamica d'areale F. s. silvestris in Italia si mostra, complessivamente, molto conservativo: un fenomeno noto anche per un altro felide, la lince eurasiatica nel suo areale alpino. La situazione sopra delineata, quindi, impone di considerare il ritrovamento di Carpegna con molta prudenza: per escludere la possibilità che si tratti delle conseguenze di un intervento antropico o di un evento naturale stocastico, occorre sviluppare un monitoraggio intensivo in adeguate aree di studio, al fine di verificare l'esistenza di una popolazione vitale del felide.

  19. Anticipation is differently expressed in rats (Rattus norvegicus) and domestic cats (Felis silvestris catus) in the same Pavlovian conditioning paradigm

    NARCIS (Netherlands)

    Bos, van den R.; Meijer, M.K.; Renselaar, van J.; Harst, van der J.E.; Spruijt, B.M.

    2003-01-01

    In rats (Rattus norvegicus) anticipation to an oncoming food reward in an appetitive Pavlovian conditioning procedure is expressed as an increase of behavioural transitions, i.e. hyperactivity. This behaviour might be related to the spontaneous appetitive behaviour of animals in relation to oncoming

  20. Post-weaning diet affects faecal microbial composition but not selected adipose gene expression in the cat (Felis catus.

    Directory of Open Access Journals (Sweden)

    Emma N Bermingham

    Full Text Available The effects of pre- (i.e., gestation and during lactation and post-weaning diet on the composition of faecal bacterial communities and adipose expression of key genes in the glucose and insulin pathways were investigated in the cat. Queens were maintained on a moderate protein:fat:carbohydrate kibbled ("Diet A"; 35:20:28% DM; n  =  4 or high protein:fat:carbohydrate canned ("Diet B"; 45:37:2% DM; n = 3 diet throughout pregnancy and lactation. Offspring were weaned onto these diets in a nested design (n  =  5 per treatment. Faecal samples were collected at wk 8 and 17 of age. DNA was isolated from faeces and bacterial 16S rRNA gene amplicons were analysed by pyrosequencing. RNA was extracted from blood (wk 18 and adipose tissue and ovarian/testicular tissues (wk 24 and gene expression levels determined using RT-qPCR. Differences (P<0.05 in composition of faecal bacteria were observed between pregnant queens fed Diet A or B. However, pre-weaning diet had little effect on faecal bacterial composition in weaned kittens. In contrast, post-weaning diet altered bacterial population profiles in the kittens. Increased (P<0.05 abundance of Firmicutes (77% vs 52% of total reads and Actinobacteria (0.8% vs 0.2% of total reads, and decreased (P<0.05 abundance of Fusobacteria (1.6% vs 18.4% of total reads were observed for kittens fed the Diet A compared to those fed Diet B post-weaning. Feeding Diet B pre-weaning increased (P<0.05 the expression levels of INRS, LEPT, PAI-1 and tended to increase GLUT1, while the expression levels of IRS-1 in blood increased in kittens fed Diet A pre-weaning. Post-weaning diet had no effect on expression levels of target genes. Correlations between the expression levels of genes involved in glucose and insulin pathways and faecal Bacteriodetes and Firmicutes phyla were identified. The reasons for why post-weaning diet affects microbial populations and not gene expression levels are of interest.

  1. Seroprevalence and Risk Factors Associated with Seropositivity to Toxoplasma gondii among Stray and Domestic Cats (Felis silvestris catus

    Directory of Open Access Journals (Sweden)

    Christel Bohn T. Garcia

    2014-12-01

    Full Text Available Toxoplasma gondii is a protozoan parasite that causes toxoplasmosis. It is widespread in the environment and infects a variety of warm-blooded animals, causing miscarriages and birth problems. Previous studies in the Philippines have determined the seropositivity of T. gondii in humans. However, the seroprevalence of the parasite among household pets, par ticularly its feline def initive host, remains insufficient . This study aimed to: (1 determine the seroprevalence of T. gondii antibodies among domestic and stray cats in the Philippines; and, (2 to analyze the risk factors associated with seropositivity. Blood samples from 59 domestic and stray cats were collected and tested for T. gondii seropositivity using a commercially available indirect ELISA kit, while pet owners and handlers were given questionnaires about their cats. Thirteen or 22.03% of the cats were seropositive to T. gondii, and risk factor analysis revealed a significant difference between domestic and stray cats with regard to diet (p = 0.026, OR = 8.333, c = 0.299 and domestication (p = 0.039, OR = 5.000, c = 0.276. Cats fed with table food tested 31.43% seropositive compared to the 4.35% of those fed with cat food, whereas 33.33% of the stray cats were seropositive compared to 7.69% for domestic cats. Odds ratio test showed that the risk factors studied were associated with higher likelihood of T. gondii seropositivity. These results implicate diet and environment in the transmission dynamics of T. gondii among cats.

  2. Anatomia dental de cães (Canis familiaris) e gatos (Felis catus). Considerações cirúrgicas

    OpenAIRE

    Juliana Kowalesky

    2005-01-01

    Com a rápida evolução da odontologia veterinária na última década, faz-se crescente a busca pelo conhecimento da área e a busca por referências bibliográficas, porém nota-se que as informações existentes de uma área essencial, a anatomia, estão limitadas a descrições básicas. Sabe-se que o conhecimento da anatomia do sistema estomatognático é de fundamental importância para que clínicos e cirurgiões possam diagnosticar e instituir tratamento adequado e preciso. Apesar dos livros de odontologi...

  3. Sequence analysis of feline caliciviruses isolated from the oral cavity of clinically normal domestic cats (Felis catus) in Florida.

    Science.gov (United States)

    Weeks, M L; Gallagher, A; Romero, C H

    2001-12-01

    Four isolates (7.3 per cent) of feline calicivirus (FCV), from oropharyngeal swabs taken from 55 unvaccinated apparently healthy cats, were identified by electron microscopy and reverse transcription-polymerase chain reaction (RT-PCR). A 671-bp fragment, comprising part of region B, all of regions C, D, and E and part of region F of the FCV capsid protein gene, was amplified from each isolate by RT-PCR and cloned for sequence analysis. Amino acid sequence comparison of these regions revealed significant sequence divergence from the F9 vaccine strain within regions C, E, and F. The hypervariable region E of the four Florida isolates and the NADC isolate contained three fewer amino acids than the commonly used F9 vaccine strain. This work provides support to the idea that currently circulating FCV strains may differ substantially from presently used vaccine strains.

  4. Efficacy of imidacloprid + moxidectin and selamectin topical solutions against the KS1 Ctenocephalides felis flea strain infesting cats

    Directory of Open Access Journals (Sweden)

    Dryden Michael W

    2011-09-01

    Full Text Available Abstract Background Two studies were conducted to evaluate and compare the efficacy of imidacloprid + moxidectin and selamectin topical solutions against the KS1 flea strain infesting cats. In both studies the treatment groups were comprised of non-treated controls, 6% w/v selamectin (Revolution®; Pfizer Animal Health topical solution and 10% w/v imidacloprid + 1% w/v moxidectin (Advantage Multi® for Cats, Bayer Animal Health topical solution. All cats were infested with 100 fleas on Days -2, 7, 14, 21, and 28. The difference in the studies was that in study #1 efficacy evaluations were conducted at 24 and 48 hours post-treatment or post-infestation, and in study #2 evaluations were conducted at 12 and 24 hours. Results In study #1 imidacloprid + moxidectin and the selamectin formulation provided 99.8% and 99.0% efficacy at 24 hours post-treatment. On day 28, the 24 hour efficacy of the selamectin formulation dropped to 87.1%, whereas the imidacloprid + moxidectin formulation provided 98.9% efficacy. At the 48 hour assessments following the 28 day infestations, efficacy of the imidacloprid + moxidectin and selamectin formulations was 96.8% and 98.3% respectively. In study # 2 the efficacy of the imidacloprid + moxidectin and selamectin formulations 12 hours after treatment was 100% and 69.4%, respectively. On day 28, efficacy of the imidacloprid + moxidectin and selamectin formulations 12 hours after infestation was 90.2% and 57.3%, respectively. In study #2 both formulations provided high levels of efficacy at the 24 hour post-infestation assessments, with selamectin and imidacloprid + moxidectin providing 95.3% and 97.5% efficacy, following infestations on day 28. Conclusions At the 24 and 48 hour residual efficacy assessments, the imidacloprid + moxidectin and selamectin formulations were similarly highly efficacious. However, the imidacloprid + moxidectin formulation provided a significantly higher rate of flea kill against the KS1 flea strain infesting cats at every 12 hour post-infestation residual efficacy assessment. Both formulations should provide excellent flea control for an entire month on cats.

  5. Haematology and biochemistry values of captive sand cats (Felis margarita) in Al Ain Wildlife Park and Resort, United Arab Emirates

    Institute of Scientific and Technical Information of China (English)

    Stephen Chege; Arshad Toosy; Judith Howlett; Ahmed Saker; John Kagira

    2013-01-01

    Objective: To investigate the haematology and biochemistry values of apparently healthy captive sand cats kept in Al Ain Wildlife Park and Resort, United Arab Emirates, with a view to establishing baseline values.Methods:Blood was collected from the femoral vein using aseptic techniques, kept in a cool box and sent to laboratory for analysis. The blood was analysed for haematological and biochemical values using veterinary hematology and chemistry analysers (ABX ABC Vet, Horiba ABX SAS Montpellier, France). Results: Haematological values were within the normal ranges recorded in domestic cats and there was no statistical difference between values found in males and females. Aspartate aminotransferase values were higher (P0.05) between males and females values.Conclusions:Our results present reference ranges for haematology and biochemistry parameters in captive sand cats. These values will be important for diagnosis of various diseases and monitoring of treatments.

  6. Coinfection by Toxoplasma gondii and Leishmania spp. in domestic cats (Felis catus in State of Mato Grosso do Sul

    Directory of Open Access Journals (Sweden)

    Audrey Rennó Campos Braga

    2014-12-01

    Full Text Available Introduction Leishmaniasis and toxoplasmosis are important to public health. Methods Antibodies for Toxoplasma gondii and Leishmania spp. were evaluated in cats from Campo Grande, State of Mato Grosso do Sul, Brazil, a region endemic for canine visceral leishmaniasis. Serum samples from 50 asymptomatic cats were titrated for T. gondii by the immunofluorescence antibody test and modified agglutination test and for Leishmania spp. by the immunofluorescence antibody test. Results These two agents coinfected two (4% of the 50 tested animals. Conclusions These findings demonstrate the concomitant presence of two important zoonoses in cats from Brazilian endemic regions for canine visceral leishmaniasis.

  7. Taxonomy Icon Data: domestic cat [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available tris_catus_NS.png http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Felis+silvestris+catus&t=L http://biosciencedbc.jp/taxonomy..._icon/icon.cgi?i=Felis+silvestris+catus&t=NL http://biosciencedbc.jp/taxonomy..._icon/icon.cgi?i=Felis+silvestris+catus&t=S http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Felis+silvestris+catus&t=NS ...

  8. Mercury in fishes of the J.N. "Ding" Darling National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From June 26 to 30,1990 spotted gar (Lepisosteus oculatus), spotted seatrout (Cynoscion nebulosus), gafftopsail catfish (Bagre marinus), and largemouth bass...

  9. Mercury concentrations in gafftopsail catfish and other fishes in waters adjacent to Hobe Sound National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From September 22-26, 1990, 20 gafftopsail catfish (Bagre marinus) and 3 other fish species (n=4) were collected from marine waters adjacent to Hobe Sound National...

  10. Glycolytic enzyme activity is essential for domestic cat (Felis catus) and cheetah (Acinonyx jubatus) sperm motility and viability in a sugar-free medium.

    Science.gov (United States)

    Terrell, Kimberly A; Wildt, David E; Anthony, Nicola M; Bavister, Barry D; Leibo, S P; Penfold, Linda M; Marker, Laurie L; Crosier, Adrienne E

    2011-06-01

    We have previously reported a lack of glucose uptake in domestic cat and cheetah spermatozoa, despite observing that these cells produce lactate at rates that correlate positively with sperm function. To elucidate the role of glycolysis in felid sperm energy production, we conducted a comparative study in the domestic cat and cheetah, with the hypothesis that sperm motility and viability are maintained in both species in the absence of glycolytic metabolism and are fueled by endogenous substrates. Washed ejaculates were incubated in chemically defined medium in the presence/absence of glucose and pyruvate. A second set of ejaculates was exposed to a chemical inhibitor of either lactate dehydrogenase (sodium oxamate) or glyceraldehyde-3-phosphate dehydrogenase (alpha-chlorohydrin). Sperm function (motility and acrosomal integrity) and lactate production were assessed, and a subset of spermatozoa was assayed for intracellular glycogen. In both the cat and cheetah, sperm function was maintained without exogenous substrates and following lactate dehydrogenase inhibition. Lactate production occurred in the absence of exogenous hexoses, but only if pyruvate was present. Intracellular glycogen was not detected in spermatozoa from either species. Unexpectedly, glycolytic inhibition by alpha-chlorohydrin resulted in an immediate decline in sperm motility, particularly in the domestic cat. Collectively, our findings reveal an essential role of the glycolytic pathway in felid spermatozoa that is unrelated to hexose metabolism or lactate formation. Instead, glycolytic enzyme activity could be required for the metabolism of endogenous lipid-derived glycerol, with fatty acid oxidation providing the primary energy source in felid spermatozoa.

  11. Oxidative phosphorylation is essential for felid sperm function, but is substantially lower in cheetah (Acinonyx jubatus) compared to domestic cat (Felis catus) ejaculate.

    Science.gov (United States)

    Terrell, Kimberly A; Wildt, David E; Anthony, Nicola M; Bavister, Barry D; Leibo, S P; Penfold, Linda M; Marker, Laurie L; Crosier, Adrienne E

    2011-09-01

    Compared with the normospermic domestic cat, sperm metabolic function is compromised in the teratospermic cat and cheetah, but the pathway(s) involved in this deficiency are unknown. Glycolysis is essential for sperm motility, yet it appears to function normally in spermatozoa of either species regardless of structural morphology. We conducted a comparative study to further understand the mechanisms of energy production in felid spermatozoa, with the hypothesis that oxidative phosphorylation is required for normal sperm function and is impaired in teratospermic ejaculates. Electroejaculates from both species were stained with MitoTracker to quantify mitochondrial membrane potential (MMP) or were incubated to assess changes in sperm function (motility, acrosomal integrity, and lactate production) after mitochondrial inhibition with myxothiazol. Sperm midpiece dimensions also were quantified. Sperm mitochondrial fluorescence (directly proportional to MMP) was ~95% lower in the cheetah compared with the normospermic and teratospermic cat, despite the cheetah having a 10% longer midpiece. In both species, MMP was increased 5-fold in spermatozoa with retained cytoplasm compared with structurally normal cells. Inhibition of oxidative phosphorylation impaired sperm function in both species, but a 100-fold higher inhibitor concentration was required in the cat compared with the cheetah. Collectively, findings revealed that oxidative phosphorylation was required for sperm function in the domestic cat and cheetah. This pathway of energy production appeared markedly less active in the cheetah, indicating a species-specific vulnerability to mitochondrial dysfunction. The unexpected, cross-species linkage between retained cytoplasmic droplets and elevated MMP may reflect increased concentrations of metabolic enzymes or substrates in these structures.

  12. Correlation between the age of the conceptus and various ultrasonographic measurements during the first 30 days of pregnancy in domestic cats (Felis catus).

    Science.gov (United States)

    Zambelli, D; Castagnetti, C; Belluzzi, S; Bassi, S

    2002-05-01

    We ultrasonographically evaluated the prenatal development in cats, from the early phases to Day 30 of pregnancy, subjecting a group of pregnant cats (n = 12) to a daily ultrasonographic exam. The ultrasonographic images allowed us to measure the minor diameter of the gestational sac and the crown-rump length of the embryo/fetus. Ten subjects underwent ovariohysterectomy at specific intervals during the pregnancy, with the aim of comparing the ultrasonographic data with real data; only two subjects brought their pregnancy to term. The earliest ultrasonographic observation of the gestational sac was on Day 10 after mating, while the embryo could be measured only beginning with Day 18. This study allowed to gather useful new data in order to clinically monitor the normal course of pregnancy in cats and to date the gestational age.

  13. The molecular detection of relaxin and its receptor RXFP1 in reproductive tissue of Felis catus and Lynx pardinus during pregnancy.

    Science.gov (United States)

    Braun, Beate C; Vargas, Astrid; Jewgenow, Katarina

    2012-03-01

    Relaxin acts as a pregnancy-specific signal in feline species, but specific information about protein structure and binding is essential for the improvement of pregnancy diagnosis in endangered feline species, like the Iberian lynx. To generate a felid-specific relaxin antibody, the DNA and protein sequences of lynx and cat were determined and peptides were chosen for antibody generation. In addition, relaxin and relaxin receptor (RXFP1) mRNA expressions were measured in uteri and ovaries of pregnant domestic cats and lynx placentae. Using real-time PCR and immunohistochemistry, it was established that feline placenta is the main source of relaxin during pregnancy. In other tested tissues, relaxin mRNA expression was weak. The RXFP1 mRNA expression was found mainly in cat uterine tissue and feline placentae. It was assumed that these tissues were main targets for relaxin. In the ovary, relaxin immunostaining was associated with blood vessels, signifying its role in vascularization.

  14. Comparison of efficiency between two artificial insemination methods using frozen-thawed semen in domestic cat (Felis catus): artificial insemination in domestic cats.

    Science.gov (United States)

    Villaverde, Ana Izabel Silva Balbin; Melo, Cely Marini; Martin, Ian; Ferreira, Tatiana Henriques; Papa, Frederico Ozanam; Taconeli, Cesar Augusto; Lopes, Maria Denise

    2009-09-01

    The aim of this study was to compare the efficiency of the intravaginal (IVAI) vs. intrauterine artificial insemination (IUAI) using frozen-thawed sperm in the domestic cat. Semen was collected from two tom cats using an artificial vagina and samples were assessed for motility (computer-assisted sperm analysis (CASA)), sperm morphology and plasma membrane integrity. After dilution with TRIS/OEP/YOLK (4% of glycerol), sperm samples were loaded into 0.25 mL straws (25 x 10(6)motile sperm/straw), incubated at 5 degrees C for 20 min and cryopreserved over liquid nitrogen (LN(2)) vapor for 15 min and then immersed in LN(2). For each AI, four straws from the same male were thawed (12s at 46 degrees C) and centrifuged at 250 x g for 8 min to pellet the sperm. The supernatant was discarded and sperm pellet resuspended with the remaining liquid, approximately 100 microL, and analyzed as described above. Queens were treated with a single im injection of 100 IU eCG to induce ovarian follicular development. Final oocyte maturation and ovulation was induced with 100 IU hCG given im at 82-84 h after eCG administration. Thirty hours after hCG administration, females were inseminated either intrauterine (n=8 queens) or intravaginally (n=8 queens), using thawed sperm from a single male. Although a pronounced decrease in sperm motility, acrosome and plasma membrane integrity was observed in sperm samples from both cats, a pregnancy rate of 75% was achieved when using the intrauterine AI method compared with 0% pregnancy when inseminated intravaginally.

  15. When cats' ways of life interact with their viruses: a study in 15 natural populations of owned and unowned cats (Felis silvestris catus).

    Science.gov (United States)

    Hellard, E; Fouchet, D; Santin-Janin, H; Tarin, B; Badol, V; Coupier, C; Leblanc, G; Poulet, H; Pontier, D

    2011-09-01

    In natural populations, virus circulation is influenced by host behavior and physiological characteristics. Cat populations exhibit a great variability in social and spatial structure, the existence of different ways of life within a same population may also result in different epidemiological patterns. To test this hypothesis, we used a logistic regression to analyze the risk factors of Feline immunodeficiency virus (FIV), feline herpes virus (FHV), feline calicivirus (FCV), and feline parvovirus (FPV) infection in owned (fed and sheltered) and unowned (neither fed nor sheltered, unsocialized) cats living in a rural environment in the North Eastern part of France. A serological survey was carried out in 492 non-vaccinated and non-sterilized individuals from 15 populations living in the same area. The prevalence of feline leukemia virus (FeLV) was also studied, but too few were infected to analyze the risk factors of this virus. For each virus, the epidemiological pattern was different in owned and unowned cats. Unowned cats were more frequently infected by directly transmitted viruses like FIV, FHV and FCV (21.22%, 67.66%, 86.52% in unowned cats vs 9.55%, 53.88%, 77.18% in owned cats, respectively), a difference that may be explained by a more solitary and more aggressive behavior in unowned adults, and/or possibly by a higher sensitivity related to a more stressful life. On the contrary, owned cats were more frequently infected with FPV (36.41% in owned cats vs 15.61% in unowned cats), possibly as a result of their concentration around human settlements. The present study showed that owned and unowned cats living in a same area have behavioral and physiological characteristics sufficiently different to influence virus circulation. Pooling different types of cats in a single sample without taking it into account could give a wrong picture of the epidemiology of their viruses. The conclusion of this work can be extended to any epidemiological studies led in wildlife species with flexible behavior as any variations in social or spatial structure, between or within populations, could result in different virus circulation.

  16. Uso de la ecografía para el bloqueo de nervios periféricos del miembro torácico en el gato (Felis catus L:)

    OpenAIRE

    2016-01-01

    Objetivos 1. Describir los abordajes ecográficos para la evaluación del plexo braquial (PB) y los principales nervios del miembro torácico, así como la anatomía y apariencia ecográfica normales correlacionando las imágenes ecográficas con la disección anatómica y las criosecciones. 2. Establecer los abordajes ecográficos para el bloqueo ecoguiado del PB. 3. Determinar la eficacia de los diferentes abordajes para el bloqueo anestésico ecoguiado del PB, mediante la evaluación de la d...

  17. Absence of zoonotic Bartonella species in questing ticks: First detection of Bartonella clarridgeiae and Rickettsia felis in cat fleas in the Netherlands

    NARCIS (Netherlands)

    Tijsse-Klasen, E.; Fonville, M.; Gassner, F.; Nijhof, A.M.; Hovius, E.K.E.; Jongejan, F.; Takken, W.; Reimerink, J.R.; Overgaauw, P.A.M.; Sprong, H.

    2011-01-01

    Background: Awareness for flea-and tick-borne infections has grown in recent years and the range of microorganisms associated with these ectoparasites is rising. Bartonella henselae, the causative agent of Cat Scratch Disease, and other Bartonella species have been reported in fleas and ticks. The r

  18. Evidence for compromised metabolic function and limited glucose uptake in spermatozoa from the teratospermic domestic cat (Felis catus) and cheetah (Acinonyx jubatus).

    Science.gov (United States)

    Terrell, Kimberly A; Wildt, David E; Anthony, Nicola M; Bavister, Barry D; Leibo, Stanley P; Penfold, Linda M; Marker, Laurie L; Crosier, Adrienne E

    2010-11-01

    Cheetahs and certain other felids consistently ejaculate high proportions (≥ 60%) of malformed spermatozoa, a condition known as teratospermia, which is prevalent in humans. Even seemingly normal spermatozoa from domestic cat teratospermic ejaculates have reduced fertilizing capacity. To understand the role of sperm metabolism in this phenomenon, we conducted a comparative study in the normospermic domestic cat versus the teratospermic cat and cheetah with the general hypothesis that sperm metabolic function is impaired in males producing predominantly pleiomorphic spermatozoa. Washed ejaculates were incubated in chemically defined medium containing glucose and pyruvate. Uptake of glucose and pyruvate and production of lactate were assessed using enzyme-linked fluorescence assays. Spermatozoa from domestic cats and cheetahs exhibited similar metabolic profiles, with minimal glucose metabolism and approximately equimolar rates of pyruvate uptake and lactate production. Compared to normospermic counterparts, pyruvate and lactate metabolism were reduced in teratospermic cat and cheetah ejaculates, even when controlling for sperm motility. Rates of pyruvate and lactate (but not glucose) metabolism were correlated positively with sperm motility, acrosomal integrity, and normal morphology. Collectively, our findings reveal that pyruvate uptake and lactate production are reliable, quantitative indicators of sperm quality in these two felid species and that metabolic function is impaired in teratospermic ejaculates. Furthermore, patterns of substrate utilization are conserved between these species, including the unexpected lack of exogenous glucose metabolism. Because glycolysis is required to support sperm motility and capacitation in certain other mammals (including dogs), the activity of this pathway in felid spermatozoa is a target for future investigation.

  19. PRESSÃO ARTERIAL MÉDIA E O FLUXO SANGÜÍNEO DA ARTÉRIA OFTÁLMICA EXTERNA EM GATOS (Felis catus Linnaeus, 1758)

    OpenAIRE

    Gentil Ferreira Gonçalves

    2005-01-01

    A medicina veterinária vem se beneficiando dos avanços tecnológicos dos meios diagnósticos em medicina, e da facilitação da divulgação da ciência. Para que novas tecnologias sejam utilizadas são necessárias informações acerca dos padrões normais para cada tipo de exame, para que se possa utilizá-lo na rotina e destiná-lo adequadamente para cada paciente. A fluxometria ultra-sonográfica está começando a ser utilizada em medicina veterinária com propósitos e fins diversos. Para destiná-la a um ...

  20. Hyoid apparatus and pharynx in the lion (Panthera leo), jaguar (Panthera onca), tiger (Panthera tigris), cheetah (Acinonyxjubatus) and domestic cat (Felis silvestris f. catus).

    Science.gov (United States)

    Weissengruber, G E; Forstenpointner, G; Peters, G; Kübber-Heiss, A; Fitch, W T

    2002-09-01

    Structures of the hyoid apparatus, the pharynx and their topographical positions in the lion, tiger, jaguar, cheetah and domestic cat were described in order to determine morphological differences between species or subfamilies of the Felidae. In the lion, tiger and jaguar (species of the subfamily Pantherinae) the Epihyoideum is an elastic ligament lying between the lateral pharyngeal muscles and the Musculus (M.) thyroglossus rather than a bony element like in the cheetah or the domestic cat. The M. thyroglossus was only present in the species of the Pantherinae studied. In the lion and the jaguar the Thyrohyoideum and the thyroid cartilage are connected by an elastic ligament, whereas in the tiger there is a synovial articulation. In adult individuals of the lion, tiger and jaguar the ventral end of the tympanohyal cartilage is rotated and therefore the ventral end of the attached Stylohyoideum lies caudal to the Tympanohyoideum and the cranial base. In newborn jaguars the Apparatus hyoideus shows a similar topographical position as in adult cheetahs or domestic cats. In adult Pantherinae, the Basihyoideum and the attached larynx occupy a descended position: they are situated near the cranial thoracic aperture, the pharyngeal wall and the soft palate are caudally elongated accordingly. In the Pantherinae examined the caudal end of the soft palate lies dorsal to the glottis. Differences in these morphological features between the subfamilies of the Felidae have an influence on specific structural characters of their vocalizations.

  1. The 18-kDa form of cat allergen Felis domesticus 1 (Fel d 1) is associated with gelatin- and fibronectin-degrading activity

    DEFF Research Database (Denmark)

    Ring, P C; Wan, H; Schou, C;

    2000-01-01

    , allergens that degrade proteins have been suggested to facilitate allergen presentation by increasing parallelular permeability of airways epithelium. However, little information exists to indicate whether Fel d 1 has other activities relevant to allergic responses. OBJECTIVE: To study whether Fel d 1......, suggest that their inhibitory action may be due to noncatalytic site interactions. Alternatively, highly purified Fel d 1 may be associated with an active contaminant, although none were found. CONCLUSION: These results suggest that Fel d 1 is another example of a domestic allergen which is associated...... with enzyme activity. It remains to be established whether the activity resides in Fel d 1 itself or in an unresolved, and possibly related, protein....

  2. Vocal correlates of sender-identity and arousal in the isolation calls of domestic kitten (Felis silvestris catus)

    OpenAIRE

    Scheumann Marina; Roser Anna-Elisa; Konerding Wiebke; Bleich Eva; Hedrich Hans-Jürgen; Zimmermann Elke

    2012-01-01

    Abstract Introduction Human speech does not only communicate linguistic information but also paralinguistic features, e.g. information about the identity and the arousal state of the sender. Comparable morphological and physiological constraints on vocal production in mammals suggest the existence of commonalities encoding sender-identity and the arousal state of a sender across mammals. To explore this hypothesis and to investigate whether specific acoustic parameters encode for sender-ident...

  3. A non-invasive monitoring on European wildcat (Felis silvestris silvestris Schreber, 1777) in Sicily using hair trapping and camera trapping: does it work?

    OpenAIRE

    2012-01-01

    An hair trapping protocol, with camera trapping surveillance, was carried out on the south-western side of the Etna, inhabited by an abundant population of the European wildcat. We aimed to collect hair for genetic analysis on the base of a field study conducted in Switzerland, where valerian tincture had been used to attract wildcats to rub again wooden sticks and therefore leaving hairs. We placed 18 hair trapping stations, plus one camera trap per scented wooden stick, 1 km away from each ...

  4. Absence of zoonotic Bartonella species in questing ticks: First detection of Bartonella clarridgeiae and Rickettsia felis in cat fleas in the Netherlands

    NARCIS (Netherlands)

    Tijsse-Klasen, E.; Fonville, M.; Gassner, F.; Nijhof, A.M.; Hovius, E.K.; Jongejan, F.; Takken, F.; Reimerink, J.R.; Overgaauw, P.A.M.; Sprong, H.

    2011-01-01

    BACKGROUND: Awareness for flea- and tick-borne infections has grown in recent years and the range of microorganisms associated with these ectoparasites is rising. Bartonella henselae, the causative agent of Cat Scratch Disease, and other Bartonella species have been reported in fleas and ticks. The

  5. Antibody detection and molecular characterization of toxoplasma gondii from bobcats (Lynx rufus), domestic cats (Felis catus), and wildlife from Minnesota, USA

    Science.gov (United States)

    Little is known of the epidemiology of toxoplasmosis in Minnesota. In this study, we evaluated Toxoplasma gondii infection in 50 wild bobcats (Lynx rufus) and 75 other animals on/near 10 cattle farms. Antibodies to T. gondii were assayed in serum samples or tissue fluids by the modified agglutinatio...

  6. Comparative speed of kill of sarolaner (Simparica™) and afoxolaner (NexGard®) against induced infestations of Ctenocephalides felis on dogs

    OpenAIRE

    Six, Robert H.; Liebenberg, Julian; Honsberger, Nicole A.; Mahabir, Sean P.

    2016-01-01

    Background Fleas are the most common ectoparasite infesting dogs globally. The many possible sequellae of infestation include: direct discomfort; allergic reactions; and the transmission of pathogens. Rapid speed of kill is an important characteristic for a parasiticide in order to alleviate the direct deleterious effects of fleas, reduce the impact of allergic responses, and break the flea infestation cycle. In this study, the speed of kill of a novel orally administered isoxazoline parasiti...

  7. Preventive efficacy of Frontline® Combo and Certifect® against Dipylidium caninum infestation of cats and dogs using a natural flea (Ctenocephalides felis infestation model

    Directory of Open Access Journals (Sweden)

    Beugnet Frederic

    2013-01-01

    Full Text Available Two studies were performed to evaluate the effectiveness of two monthly topical anti-flea products for the prevention of Dipylidium caninum infestations in cats and dogs. A single treatment with Frontline® Combo spot-on for cats (fipronil-(S-methoprene and two successive monthly treatments of Certifect® for dogs (fipronil-amitraz-(S-methoprene were assessed for the prevention of D. caninum infestations following weekly challenges of treated cats or dogs with metacestode naturally-infected fleas. The rate of infestations using the model in cats versus dogs explains the choice of a 1-month trial in cats and a 2-month trial in dogs. The experimental flea-infection model resulted in a range of 22–53% of the fleas being infected by Dipylidium cysticercoids. The arithmetic mean flea counts recorded for the untreated cats ranged from 51.2 to 68. The geometric mean flea counts recorded for the Frontline Combo treated cats differed significantly (p < 0.05 from those of the untreated control cats on all assessment days. The arithmetic mean flea counts recorded for the untreated dogs ranged from 166.6 to 238.6. The geometric mean flea counts recorded for the Certifect treated dogs differed significantly (p < 0.001 from those of the untreated group on all assessment days. Frontline Combo treatment on cats provided ≥99.8% persistent anti-flea efficacy throughout the 30-day treatment period. In the dog study, the two Certifect treatments provided ≥97% persistent efficacy throughout the 60-day study. Based on the collection of expelled D. caninum proglottids by cats, 100% (6/6 of the control cats and 0% (0/6 of Frontline Combo treated cats were infested with D. caninum. Frontline Combo spot-on for cats was therefore 100% effective in preventing infection with D. caninum. In dogs, 7 out of the 8 control group dogs (87.5% produced proglottids following infestation of infected fleas, whereas 0 out of 8 dogs (0% in the treated group were infected. The infection rates of the two groups were significantly different. The percent effectiveness for the Certifect treatment group for the prevention of D. caninum infection was 100% during this 2-month trial. No treatment-related adverse events were observed in either cats or dogs during these studies.

  8. Avaliação da pressão arterial em gatas (Felis catus submetidas a pneumoperitônio com dióxido de carbono

    Directory of Open Access Journals (Sweden)

    JEO Alves

    2016-12-01

    Full Text Available Pneumoperitônio é a técnica de insuflação com gás da cavidade peritoneal necessária para que haja uma boa visualização das estruturas anatômicas na cirurgia videolaparoscópica. Todavia, diversas alterações fisiológicas são relatadas em decorrência do pneumoperitônio, quais sejam por deslocamento e compressão de estruturas como o diafragma, alterando a fisiologia respiratória, e a veia cava caudal, comprometendo o pré-carga e o débito cardíaco, ou por efeitos sistêmicos decorrentes da absorção de CO2, por exemplo. O presente estudo teve como objetivo avaliar as alterações hemodinâmicas do pneumoperitônio com CO2 em gatas anestesiadas. Foram utilizadas 10 gatas sadias, adultas, premedicadas com cloridrato de cetamina e midazolam e anestesiadas com isoflurano e submetidas a OSH por cirurgia laparoscópica com insuflação abdominal por CO2. As pressões diastólica, sistólica e média foram avaliadas de maneira invasiva por cateterização da artéria femoral e mensurada por um monitor multiparamétrico antes e depois do estabelecimento do pneumoperitônio. Os resultados foram formatados e submetidos à análise de variância e posteriormente analisados pelo teste t de Student e teste Tukey. As alterações em PAS, PAD e PAM observadas no decorrer dos procedimentos, entretanto, não configuraram diferenças estatísticas conforme as análises realizadas. Conclui-se, então, que a realização do pneumoperitônio com CO2 não provoque alterações relevantes na pressão arterial em gatas anestesiadas ou que estas alterações sejam fisiologicamente corrigidas por mecanismos compensatórios.

  9. Anatomical study of the forearm and hand nerves of the domestic cat ( Felis catus), puma ( Puma concolor) and jaguar ( Panthera onca).

    Science.gov (United States)

    Sánchez, H L; Silva, L B; Rafasquino, M E; Mateo, A G; Zuccolilli, G O; Portiansky, E L; Alonso, C R

    2013-04-01

    The innervation of the forearm and hand regions of cats has not been well described despite its importance for any surgery or any neurological disorder. It is probably the main area where disorders of peripheral nerves in this species are observed. In felines, the forelimbs facilitate the jump and represent the most important way for capturing prey. The main muscles and nerves involved in this activity are located in the region of the forearm and hand. The aim of the present study was to provide a detailed description of the innervation of the forearm and hand regions of the jaguar and puma, in comparison with that of the domestic cat, contributing thus with the anatomical knowledge of the area for applying it to surgery and pathology. The forearms of three pumas and two jaguars (all of them fixed in formalin) and of six domestic cats (fresh) were dissected. The nerves path and their forearm distribution patterns of all three species were described. The analysed results indicate that the observed variations between species are minimal; thus, the anatomy described for domestic cats can be widely applied to American wild felids.

  10. Real-time PCR of the mammalian hydroxymethylbilane synthase (HMBS gene for analysis of flea (Ctenocephalides felis feeding patterns on dogs

    Directory of Open Access Journals (Sweden)

    Wang Chengming

    2012-01-01

    Full Text Available Abstract Background Precise data on quantitative kinetics of blood feeding of fleas, particularly immediately after contact with the host, are essential for understanding dynamics of flea-borne disease transmission and for evaluating flea control strategies. Standard methods used are inadequate for studies that simulate early events after real-life flea access to the host. Methods Here, we developed a novel quantitative polymerase chain reaction targeting mammalian DNA within fleas to quantify blood consumption with high sensitivity and specificity. We used primers and fluorescent probes that amplify the hydroxymethylbilane synthase (HMBS gene, an evolutionary divergent gene that is unlikely to be detected in insects by mammalian-specific primers and probes. To validate this assay, fleas were placed on dogs, allowed to distribute in the hair, and removed at specific time points with single-use combs. Fleas were then immediately homogenized by vigorous shaking with ceramic beads in guanidinium-based DNA preservation buffer for DNA extraction. Results The specificity of this assay was ascertained by amplification of canine, feline and equine blood with differential product melting temperatures (Tm, and lack of amplification of bovine and porcine blood and of adult fleas reared from larvae fed with bovine blood. Sensitivity of the assay was established by limiting dilution and detection of single copies of HMBS DNA equivalent to 0.043 nL blood. Application of the assay indicated that after 15 minutes on a dog, male and female fleas had ingested low, but similar amounts of approximately 1.1. nL blood. Saturation uptake of 118 and 100 nL blood per flea was found at 30 and 60 min on the dog, respectively. Conclusions The HMBS PCR method developed here offers the advantages of both exquisite sensitivity and specificity that make it superior to other approaches for quantification of blood ingested by fleas. The capability to detect minute quantities of blood in single fleas, particularly immediately after colonization of the host, will provide a superior tool for studying flea-host interactions, flea-borne disease transmission, and flea control strategies.

  11. Espectro trófico del bagre Ictalurus punctatus (Silurifomes: Ictaluridae, en la presa Lázaro Cárdenas, Indé, Durango, México Trophic spectrum of the catfish Ictalurus punctatus (Siluriformes: Ictaluridae, in the Lázaro Cárdenas reservoir, Indé, Durango, Mexico

    Directory of Open Access Journals (Sweden)

    Gabriel Fernando Cardoza Martínez

    2011-08-01

    Full Text Available Se analizó el contenido de 240 estómagos de Ictalurus punctatus (Rafinesque, 1818, colectados de 2006 a 2007, con el objetivo de determinar su espectro trófico, su variación por talla y época climática. Los contenidos estomacales se analizaron en laboratorio y los organismos se determinaron hasta nivel de orden. Los datos obtenidos fueron analizados por métodos cuantitativos y cualitativos. Los órdenes más frecuentes fueron Perciformes y Atheriniformes que corresponden a peces forraje, así como algas verdes del orden Charales. Además, se encontraron otros siete órdenes de invertebrados: Schizodonta, Odonata, Himenoptera, Orthoptera, Hemiptera, Homoptera y Scorpionida, además de dos órdenes de plantas: Poales y Fabales. El orden Perciforme constituyó casi la mitad del total del peso del contenido estomacal, seguido por los órdenes Atheriniforme y Charales. En las estaciones de verano e invierno se presentó el mayor número de estómagos vacíos, mientras que en primavera se encontró el mayor número de estómagos llenos. La cantidad de alimento ingerida en la estación de invierno fue significativamente menor que en las demás estaciones. El principal alimento en primavera, verano y otoño, fueron los peces, mientras que en invierno hubo mayor preferencia por las algas.Having the objective of determining their trophic spectrum and their variations within a year, the contents of 240 stomachs of Ictalurus punctatus (Rafinesque, 1818 were analyzed from 2006 to 2007. The items of the stomach contents were determined to the order level. The data was analyzed by different quantitative and qualitative methods. The most frequent orders were Perciforms and Atheriniforms corresponding to forage fish, along with green algae of the Charales order. Also, seven orders of invertebrates were found: Schizodonta, Odonata, Himenoptera, Orthoptera, Hemiptera, Homoptera and Scorpionida, and two orders of plants: Poales and Fabales. The Perciform order made up almost half of the total weight of the stomach contents, followed by the Atheriniform and the Charales orders. The greatest number of empty stomachs showed up during summer and winter, while the greatest number of filled-up stomachs was found during the spring season. The quantity of food consumed during winter time was significantly lower than the amount consumed during all other seasons. The kind of food consumed during the spring, summer and autumn was mainly fish, while algae were preferred during the winter season.

  12. FREQUENCY OF THE VIRUS OF THE FELINE LEUKEMIA (FeLV IN DOMESTIC FELINES (Felis catus SEMI-DOMICILED IN THE MUNICIPALITIES OF PELOTAS AND RIO GRANDE FREQUÊNCIA DO Vírus da Leucemia Felina (VLFe em FELINOS DOMÉSTICOS (Felis catus SEMIDOMICILIADOS NOS MUNICÍPIOS DE PELOTAS E RIO GRANDE

    Directory of Open Access Journals (Sweden)

    Dilmara Reischak

    2010-04-01

    Full Text Available Considering the importance of FeLV in the feline clinic, as well as the likely agent spread from a symptomatic or asymptomatic feline bearer, this work has as objective the study of the frequency of FeLV in felines residents in the cities of the Pelotas and Rio Grande, municipalities located in the south area of Brazil. For that, the blood of 120 semi-domiciled animals was collected for the detection of the retrovirus through the Indirect Immunofluorescence technique (IFA. FeLV was detected in 38,3% (46/120 of the studied animals, representing a larger frequency considering other studies accomplished in other areas of Brazil, what confirms the importance of FeLV in the studied region.

    KEY WORDS: FeLV, felines, immunofluorescence, retrovirus.

    Considerando a importância do VLFe na clínica felina, assim como a possível disseminação do agente a partir de um felino portador sintomático ou assintomático, o estudo tem como objetivo verificar a frequência de viremia pelo VLFe em felinos residentes em Pelotas e Rio Grande, municípios situados na região sul do Brasil. Para isso foi coletado sangue de 120 animais semidomiciliados para a detecção do retrovírus através da técnica de imunofluorescência indireta (IFI. Detectou-se a viremia em 38,3% (46/120 dos animais estudados, representando uma frequência maior em relação a outros estudos realizados no Brasil, o que confirma a importância deste agente na região estudada.

    PALAVRAS-CHAVES: Felinos, imunofluorescência, retrovírus, VLFe.

  13. Occurrence of Leishmania (Leishmania chagasi in a domestic cat (Felis catus in Andradina, São Paulo, Brazil: case report Ocorrência de Leishmania (Leishmania chagasi em gato doméstico (Felis catus em Andradina, São Paulo, Brasil: relato de caso

    Directory of Open Access Journals (Sweden)

    Willian Marinho Dourado Coelho

    2010-12-01

    Full Text Available This work describes natural infection by Leishmania in a domestic cat where amastigote forms of the parasite were observed in the popliteal lymph node imprint. Positive and negative serological reactions were observed by enzyme-linked immunosorbent assay (ELISA and indirect immunofluorescence assay (IFA, respectively. Polymerase chain reaction (PCR revealed that the nucleotide sequence of the sample was identical to Leishmania (L. chagasi. This is the first report of the disease in felines of the city of Andradina, SP, an area considered endemic for canine and human visceral leishmaniasis.Neste trabalho, é relatada a infecção natural por Leishmania em um gato doméstico no qual, formas amastigotas do parasito foram observadas em imprint de linfonodo poplíteo. Reações sorológicas positivas e negativas foram observadas pelo teste de imunoadsorção enzimática (ELISA e reação de imunofluorescência indireta (RIFI, respectivamente. A reação em cadeia da polimerase (PCR revelou que a sequência de nucleotídeos foi idêntica à Leishmania (L. chagasi. Este é o primeiro relato da doença em felino da cidade de Andradina, Estado de São Paulo, Brasil, área considerada endêmica para leishmaniose visceral canina e humana.

  14. Terapia floral em gatos domésticos (Felis catus, Linnaeus, 1758 portadores do complexo da doença respiratória felina: estudo clínico e hematológico Flower therapy in domestic cats (Felis catus, Linnaeus, 1758 with feline respiratory disease complex: clinical and hematological study

    Directory of Open Access Journals (Sweden)

    R.F. Araújo

    2010-12-01

    Full Text Available A terapia floral é considerada, atualmente, prática médica alternativa utilizada em diversas situações clínicas, constituindo possibilidade a mais de prevenção e cura de muitas doenças de natureza física e emocional. Este estudo objetivou pesquisar o efeito das essências do Sistema Brasileiro de Florais Compostos de Joel Aleixo num mesmo grupo de gatos domésticos com sinais clínicos sugestivos de Doença Respiratória Felina (DRF, tratados em diferentes momentos (M0, M1, M2, M3. Foram utilizados 20 gatos domésticos, de ambos os sexos, sem raça definida, com idade média de 5,63 ± 3,02 anos criados em gatil na UFRPE. Os animais foram submetidos ao tratamento com os florais por via oral em duas etapas. Na primeira etapa com os florais Desintus Total e Helminthus Total por 14 dias, e na segunda etapa com os florais Antibius e Regius por 28 dias. Os resultados observados, quanto aos aspectos clínicos, foram redução de secreção nasal, secreção ocular e estertores pulmonares; desaparecimento de sinais clínicos como fezes alteradas, úlceras na cavidade oral, pêlos eriçados e permanência da hipertrofia dos linfonodos. Quanto aos aspectos hematológicos houve interferência nas variáveis relacionadas ao hemograma (hemoglobina, VCM, CHCM, leucócitos, linfócitos e monócitos. Conclui-se que a terapia floral mostrou-se eficaz em gatos domésticos com sinais sugestivos de DRF criados nas mesmas condições de manejo.Flower therapy is currently considered an alternative medical practice used in several clinical situations, providing another way to prevent and cure many diseases of physical and emotional nature. This study aimed to investigate the effect of essences of the Brazilian Compound Flower System of Joel Aleixo in one same group of domestic cats showing suggestive clinical signs of Feline Respiratory Disease (FRD, treated in different moments (M0, M1, M2, M3. Twenty domestic cats, males and females, of mixed breed, with average age 5.63 ± 3.02, raised in the UFRPE's cattery, were used. The animals were orally treated with flower essences in two steps. Firstly with Total Desintus and Total Helminthus flower for 14 days and in the second step with Antibius and Regius for 28 days. The obtained results related to clinical aspects were a decrease in nasal and ocular discharge, and lung stertors; disappearance of clinical signal such as altered stool, ulcers in the oral cavity, bristling and hypertrophy of lymph nodes. Regarding hematological aspects, there was interference in the variables related to the blood (hemoglobin, MCV, MCHC, leucocytes, lymphocytes and monocytes. It can be concluded that flower therapy was effective in cats with signs suggestive of FRD raised under the same management conditions.

  15. COⅠ and COⅡ sequences analysis for an imported Siphonaptera sample of Ctenocephalides felis felis at Alataw Pass,China-Kazakhstan%中哈边境阿拉山口口岸输入性猫栉首蚤指名亚种线粒体COⅠ和COⅡ基因序列分析

    Institute of Scientific and Technical Information of China (English)

    罗丹; 王安东; 尹小平; 田延河; 梁臻; 巴特; 张江国

    2016-01-01

    目的 分析阿拉山口口岸地区输入性猫栉首蚤指名亚种细胞色素C氧化酶Ⅰ(COⅠ)和Ⅱ(COⅡ)基因特征和系统进化关系.方法 从2014年1月入境集装箱死猫体表采集蚤类样本,形态学鉴定完毕后提取DNA,PCR扩增COⅠ和COⅡ基因并测定序列,使用Mega 6.0通过ML法构建系统发育树.结果 猫栉首蚤指名亚种COⅠ和COⅡ基因富含A+T,碱基突变多为置换突变,无移码,缺失和插入突变;Blast显示与澳大利亚猫栉首蚤指名亚种同源性较高(99%).结论 COⅠ基因序列存在足够的变异能够区分亲缘关系很近的种类,为外来或新发现的蚤种的鉴别提供了分子水平的技术依据.

  16. COMPARISON BETWEEN TWO METHODS OF STAINING FOR ASSESSMENT OF MORPHOLOGY AND ACROSOME IN DOMESTIC CAT (Felis catus SPERMATOZOA COMPARAÇÃO ENTRE DOIS MÉTODOS DE COLORAÇÃO PARA ANÁLISE MORFOLÓGICA E ACROSSOMAL DE ESPERMATOZÓIDES DE GATO DOMÉSTICO (Felis catus.

    Directory of Open Access Journals (Sweden)

    Frederico Ozanam Papa

    2008-10-01

    Full Text Available

    The objective of this study was to evaluate the efficiency of the modified Karras staining technique (KA to analyze domestic cat sperm morphology by comparing it with the Fast Green FCF/ Rose Bengal staining (FR, previously used for this species. Four adult cats were used, from which sperm samples were collected four times in alternate days for each tom using an artificial vagina (n=16 ejaculates. Both staining techniques were performed for each ejaculate. For the FR staining technique, the semen in natura was diluted in 2.9% sodium citrate and, afterwards, in the staining solution. After 70 seconds, smears were made onto slide and dried at 37ºC. For the KA staining technique, previously made and formol saline fixed slides were sequentially immersed in Rose Bengal solution, Tannin solution, and Victoria Blue B solution, and dried at room temperature. For sperm evaluation, 200 sperm cells were assessed for each staining technique in all ejaculate samples using a bright field microscope at 1000X magnification. Statistical analysis used the non-parametric Wilcoxon test, establishing significance at p<0.05. For the KA staining technique, higher percentage of distal cytoplasmic droplets and lower percentage of sperm head defects were obtained when compared to the FR staining technique. This way, both staining techniques were not totally efficient for the assessment of morphological defects found in the domestic cat in natura spermatozoa.

    KEY WORDS: Acrosome, domestic cat, spermatozoa, sperm morphology, staining.

     

    O objetivo do estudo foi avaliar a eficiência do método de coloração Karras modificado (KA para a análise da morfologia espermática no gato doméstico através da comparação com a coloração Fast Green FCF/Rosa Bengala (FR, previamente utilizada para esta espécie. Utilizaram-se quatro gatos adultos, colhendo-se quatro vezes amostras de sêmen em dias alternados para cada animal através de vagina artificial (n=16 ejaculados. Para cada ejaculado, realizaram-se duas colorações. Para a coloração FR, o sêmen in natura foi diluído em citrato de sódio 2,9% e, posteriormente, em solução de coloração. Após setenta segundos, procedeu-se a esfregaços em lâminas, as quais foram secas a 37ºC. Para a coloração KA, os esfregaços previamente confeccionados e fixados em formol salino foram imersos seqüencialmente nas soluções de Rosa Bengala, Tanino e Azul Vitória e secas em temperatura ambiente. Avaliaram-se duzentas células para cada tipo de coloração em todos os ejaculados, usando-se microscópio de luz em aumento de 1.000X. Efetuou-se análise estatística mediante o teste não-paramétrico de Wilcoxon, estabelecendo diferença significativa quando p<0,05. Para a coloração de KA, obtiveram-se maior porcentagem de gota citoplasmática distal e menor porcentagem de defeitos de cabeça quando comparada à coloração FR. Assim, nenhuma das colorações mostrou-se totalmente eficiente na identificação dos defeitos de morfologia encontrados na avaliação do sêmen in natura de gatos domésticos.

    PALAVRAS-CHAVES: Acrossomo, coloração, espermatozóide, gato doméstico, morfologia espermática.

     

  17. Manual de métodos parasitológicos e histopatológicos en piscicultura

    OpenAIRE

    Keim, Albert

    1982-01-01

    Recientes problemas provocados por epiemias de infecciones de Ichthyophthirius multifiliie en la cría de bagre negro (Rhandia sapo) cultivado por el Departamento de Acuicultura del INAPE de Uruguay, y el conocimiento de que todos los piscicultores tienen problemas con los parásitos, constituyeron la base y antecedente para el inicio de un programa de investigaciones parasitológicas. Este programa incluyó. 1) Disección de bagres capturados en Laguna de Sauce y colecta de parásitos. 2) Disecció...

  18. In situ assessment of two catfish species (pisces, Ariidae) to evaluate pollution in a harbor

    Science.gov (United States)

    Neta, Raimunda Nonata Fortes Carvalho; Junior, Audalio Rebelo Torres; Sousa, Débora Batista Pinheiro; de Sousa de Oliveira Mota Andrade, Ticianne; Torres, Hetty Salvino; da Silva Castro, Jonatas; da Silva de Almeida, Zafira; Santos, Débora Martins Silva; Tchaicka, Lígia

    2016-12-01

    A histopathological and biometric database for the catfish Sciades herzbergii and Bagre bagre from São Luís Island (Harbor area) and Caranguejos Island (reference area) in Brazil is presented. Branchial and hepatic lesions were classified into three reaction patterns: 1) circulatory or inflammatory disturbances; 2) regressive changes; 3) progressive changes. The total length (Lt), standard length (Ls), furcal length (Lf), total weight (Wt), and gonad weight (Wg) of each fish were recorded. As expected, most populations of catfish considered in this study are highly heterogeneous, with lengths and weights deviating from the reference sample. No histopathological lesions were observed in Sciades herzbergii examined at the reference site (Caranguejos Island). In contrast, 90% of the catfish S. herzbergii from sites located in the Harbor Area (São Luís Island) had one or more types of branchial and hepatic lesions. As opposed to what was observed in S. herzbergii, more than 86.33% of Bagre bagre individuals showed histopathological alterations in both areas. The utility of histopathological lesions and biometric data as sensitive indicators of the health of wild catfish populations has been demonstrated. Sciades herzbergii proved to be a better species for biomonitoring because it was able to differentiate one impacted site (Port Area/ São Luís Island) from a region relatively free of contaminants (Reference Area/ Caranguejos Island).

  19. Monogenoidea (Polyonchoinea: Dactylogyridae) parasitizing the gills of marine catfish (Siluriformes: Ariidae) inhabiting the Atlantic Amazon Coast of Brazil.

    Science.gov (United States)

    Domingues, Marcus V; Soares, Geusivam B; Watanabe, Alana

    2016-06-23

    A parasitological survey of monogenoids infesting the gills of marine catfish (Ariidae) captured from the Atlantic coastal region of the Amazon Basin was carried out during the 2011-2013 period. The gills of 448 specimens involving twelve ariid species (29 Amphiarius rugispinis (Valenciennes), 52 Aspistor quadriscutis (Valenciennes), 74 Bagre bagre (Linnaeus), 16 Cathorops arenatus (Valenciennes), 13 Cathorops agassizii (Eigenmann & Eigenmann), 17 Cathorops spixii (Agassiz), 3 Cathorops sp., 13 Notarius grandicassis (Valenciennes), 14 Sciades couma (Valenciennes), 64 Sciades herzbergii (Bloch), 48 Sciades parkeri (Traill), 13 Sciades passany (Valenciennes), 92 Sciades proops (Valenciennes) were sampled. No monogenoids were found in Cathorops agassizi, Cathorops arenatus, Cathorops spixii, Cathorops sp. and Sciades parkeri, but the gills of the other sampled species were parasitized by at least one species of monogenoid. We identified four new species of Chauhanellus and one new species of Hamatopeduncularia: Chauhanellus hamatopeduncularoideum n. sp. from Amphiarius rugispinis and Sciades couma; Chauhanellus hypenocleithrum n. sp. from Sciades proops; Chauhanellus susamlimae n. sp. from Sciades herzbergii and Sciades passany; Chauhanellus velum n. sp. from Sciades couma, Sciades herzbergii and Sciades passany; and Hamatopeduncularia cangatae n. sp. from Aspistor quadriscutis and Notarius grandicassis. Four previously described species were reported for the first time parasitizing ariids from Atlantic Amazon: Chauhanellus neotropicalis Domingues & Fehlauer, 2006 from Amphiarius rugispinis, Aspistor quadriscutis, Notarius grandicassis and Sciades passany; Chauhanellus boegeri Domingues & Fehlauer, 2006 from Sciades couma and Sciades herzbergii; Hamatopeduncularia bagre Hargis, 1955 from Bagre bagre; and Neomurraytrematoides proops Zambrano & Añez 1993 from Sciades passany. The monotypic Neomurraytrematoides Zambrano & Añez 1993 was placed in synonymy with

  20. Origem, ramificação e distribuição fascicular do nervo radial no braço do gato doméstico (Felis catus domesticus, Linnaeus, 1758)

    OpenAIRE

    Guimarães, Gregório Corrêa

    2004-01-01

    Estudou-se a origem, a distribuição e a ramificação do nervo radial de 35 gatos domésticos adultos, 17 machos e 18 fêmeas, sem raça definida, mediante dissecações macro e mesoscópicas, após a fixação dos espécimes em solução aquosa de formaldeído a 10 %, e também realizou-se o exame histológico do referido nervo. O nervo radial mostrou-se polifasciculado desde sua origem até a divisão em ramos superficial e profundo. Originou-se do C6 ao T1 em 30 % dos exemplares e do C7 ao T1 em 70 % dos exe...

  1. Estudo retrospectivo das afecções orais em 754 felinos domésticos (Felis catus) atendidos no Laboratório de Odontologia Comparada da Universidade de São Paulo

    OpenAIRE

    Mariana Suemi Fugita

    2016-01-01

    Objetivou-se fazer um estudo retrospectivo avaliando quais as afecções da cavidade oral foram mais frequentes nos gatos domésticos atendidos no Laboratório de Odontologia Comparada da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, relatando estatisticamente a prevalência das afecções da cavidade oral de gatos, enfatizando se há correlação entre elas e com características como raça, sexo, faixa etária e estado reprodutivo. Os dados analisados dos 754 prontuários fo...

  2. Efecto del fotoperíodo y de la administración de melatonina sobre la producción espermática en el gato doméstico (Felis silvestris catus)

    OpenAIRE

    Núñez Favre, Romina de los Ángeles

    2013-01-01

    En el felino doméstico, la estacionalidad ovulatoria y estral de la hembra ocurre durante los días que presentan más de 12 h luz. Sin embargo, la estacionalidad reproductiva del gato ha sido definida recientemente. El objetivo de esta tesis fue estudiar el efecto del fotoperiodo natural, el manejo lumínico artificial y la administración de melatonina sobre la producción espermática en el gato doméstico. En el primer estudio se evaluó el efecto del fotoperiodo natural sobre la morfología testi...

  3. DETECTION OF RICKETTSIA FELIS LIKE AGENT FROM EULAELAPS STABULARIS KOCH%从厩真厉螨中检出与猫立克次体近缘的立克次体核酸片段

    Institute of Scientific and Technical Information of China (English)

    姚李四; 刘勇先; 邵丽筠; 张晓龙; 杨宇; 王静; 徐宝梁; 王宝麟; 赵彤言

    2012-01-01

    本研究用立克次体属特异的gltA和ompB基因扩增引物,从吉林长白县捕获鼠中分拣的534只厩真厉螨中扩得gltA和ompB基因片段.通过基因片段的序列测定、BLAST比对和系统发育分析,显示两个扩增基因与猫立克次体Rickettsia felis同源性最高(99%),证明该地区厩真厉螨携带与猫立克次体近缘的立克次体.%The segments of glt A gene and omp B gene were amplified and identified with Rickettsia specific git A and omp B primers from 534 mites (Eulaelaps stabularis Koch) collected from rodents of Changbai County, Jilin Province, China. By sequencing, BLAST qualifying and phylogenetic analyzing, it was revealed that the two amplified segments were of highest homology to Rickettsia fells (99% ) , implying that the locally collected E. Stabularis may carry some Rickettsia sp. Allied to Rickettsia fells.

  4. Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections in stray and pet cats (Felis catus) in northwest China: co-infections and risk factors.

    Science.gov (United States)

    Cong, Wei; Meng, Qing-Feng; Blaga, Radu; Villena, Isabelle; Zhu, Xing-Quan; Qian, Ai-Dong

    2016-01-01

    This study was conducted to estimate the prevalence of Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections among stray and pet cats in Lanzhou, northwest China, and to identify the influence of age, gender, and regions on seropositivity. T. gondii antibodies were examined in cat sera by the modified agglutination test (MAT). The circulating antigens of D. immitis and FeLV and specific antibodies to FIV were examined using kits commercially available. The overall prevalence of T. gondii, FIV, FeLV, and D. immitis was 19.34, 9.12, 11.33, and 3.04 %, respectively. For the genetic characterization of T. gondii genotypes in cats, genomic DNA was extracted from the seropositive cats and the T. gondii B1 gene was amplified using a semi-nested PCR. DNA samples giving positive B1 amplification were then genotyped using multilocus PCR-RFLP. Two T. gondii genotypes (ToxoDB#9 and ToxoDB#1) were identified. Results of the multivariate logistic regression analysis showed that older cats are more likely to be seropositive than juveniles for T. gondii, FIV, FeLV, and D. immitis. This is the first report of T. gondii genotypes in cats in northwest China. Moreover, the present study is the first study of retrovirus and D. immitis seroprevalence in cats in China. The results revealed that T. gondii, FIV, and FeLV infections are common in stray and pet cats in northwest China.

  5. Lesiones causadas por helmintos del aparato digestivo en peces estuarinos de la Laguna Tres Palos, Guerrero, México

    OpenAIRE

    Ramón Vázquez Núñez; José Ramírez Lezama; David Osorio Sarabia; Larisa Adriana Chávez Soriano; Fernando Constantino Casas

    2004-01-01

    Se realizó el presente estudio con el fi n de identificar y comparar las lesiones macro y microscópicas asociadas a helmintos en el tracto digestivo del Ariopsis guatemalensis (“cuatete”) y Eleotris picta (“alahuate”) de la Laguna de Tres Palos, Guerrero, México. En Mayo, Julio y Diciembre del 2001 se colectaron 30 peces de cada especie. A 15 individuos de cada muestra se les practicó el examen histopatológico y a los 15 restantes el examen parasitológico. Se identifi caron tres grupos de hel...

  6. Parasites of domestic and wild animals in South Africa. XLIV. Fleas (Insecta : Siphonaptera : Pulicidae collected from 15 carnivore species

    Directory of Open Access Journals (Sweden)

    I.G. Horak

    2004-11-01

    Full Text Available Fleas were collected from 61 wild carnivores belonging to 13 species in various nature reserves and on farms, two feral domestic cats in a nature reserve and a domestic dog in the city of Johannesburg. Eleven flea species, including two subspecies of one of these, belonging to six genera were recovered. Amongst these only Ctenocephalides felis felis and Ctenocephalides felis strongylus are considered specific parasites of carnivores. The remaining ten species normally infest the prey animals of the various carnivores.

  7. NCBI nr-aa BLAST: CBRC-CFAM-12-0016 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CFAM-12-0016 ref|NP_001009331.1| cannabinoid receptor 1 [Felis catus] sp|O02777|CNR1_FELCA Canna...binoid receptor 1 (CB1) (CB-R) gb|AAB53440.1| CB1 cannabinoid receptor [Felis catus] NP_001009331.1 0.0 97% ...

  8. NCBI nr-aa BLAST: CBRC-RNOR-05-0081 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-05-0081 ref|NP_001009331.1| cannabinoid receptor 1 [Felis catus] sp|O02777|CNR1_FELCA Canna...binoid receptor 1 (CB1) (CB-R) gb|AAB53440.1| CB1 cannabinoid receptor [Felis catus] NP_001009331.1 0.0 96% ...

  9. NCBI nr-aa BLAST: CBRC-SARA-01-0195 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-SARA-01-0195 ref|NP_001009331.1| cannabinoid receptor 1 [Felis catus] sp|O02777|CNR1_FELCA Canna...binoid receptor 1 (CB1) (CB-R) gb|AAB53440.1| CB1 cannabinoid receptor [Felis catus] NP_001009331.1 0.0 96% ...

  10. 77 FR 60622 - New Animal Drugs; Change of Sponsor's Address; Monensin; Spinosad; Tilmicosin

    Science.gov (United States)

    2012-10-04

    .... infestations (Ctenocephalides felis) for 1 month on cats and kittens 14 weeks of age and older and 2 pounds of... infestations (C. felis) for 1 month on cats and kittens 14 weeks of age and older and 2 pounds of body...

  11. NCBI nr-aa BLAST: CBRC-PHAM-01-1218 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PHAM-01-1218 ref|YP_246709.1| cell surface antigen Sca3 [Rickettsia felis URRW...XCal2] gb|AAY61544.1| Cell surface antigen Sca3 [Rickettsia felis URRWXCal2] YP_246709.1 0.99 23% ...

  12. Considerações sobre pulgas (Siphonaptera da raposa Cerdocyon thous (Canidae da área endêmica de leishmaniose visceral de Jacobina, Bahia, Brasil

    Directory of Open Access Journals (Sweden)

    Cerqueira Elúzio J.L.

    2000-01-01

    Full Text Available No período de julho a setembro de 1998 foram coletadas 152 pulgas em 18 exemplares da raposa Cerdocyon thous capturados na área endêmica de leishmaniose visceral de Jacobina, Estado da Bahia. As pulgas foram identificadas como: 136 Rhopalopsyllus lutzi lutzi, 11 Pulex irritans, 2 Ctenocephalides canis, 1 Ctenocephalides felis felis e 2 Xenopsylla cheopis.

  13. NCBI nr-aa BLAST: CBRC-TBEL-01-1883 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TBEL-01-1883 ref|NP_001009331.1| cannabinoid receptor 1 [Felis catus] sp|O02777|CNR1_FELCA Canna...binoid receptor 1 (CB1) (CB-R) gb|AAB53440.1| CB1 cannabinoid receptor [Felis catus] NP_001009331.1 0.0 97% ...

  14. NCBI nr-aa BLAST: CBRC-LAFR-01-1734 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-LAFR-01-1734 ref|NP_001009331.1| cannabinoid receptor 1 [Felis catus] sp|O02777|CNR1_FELCA Canna...binoid receptor 1 (CB1) (CB-R) gb|AAB53440.1| CB1 cannabinoid receptor [Felis catus] NP_001009331.1 0.0 90% ...

  15. NCBI nr-aa BLAST: CBRC-FCAT-01-1020 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FCAT-01-1020 ref|NP_001009331.1| cannabinoid receptor 1 [Felis catus] sp|O02777|CNR1_FELCA Canna...binoid receptor 1 (CB1) (CB-R) gb|AAB53440.1| CB1 cannabinoid receptor [Felis catus] NP_001009331.1 0.0 98% ...

  16. Candidatus ‘Rickettsia senegalensis’ in cat fleas in Senegal

    Directory of Open Access Journals (Sweden)

    O. Mediannikov

    2015-01-01

    Full Text Available Epidemiological studies of Rickettsia felis and related bacteria are very important, because the natural cycle of this important infection has not yet been established. The recent emergence of R. felis-associated febrile diseases in West and East Africa demands insightful epidemiological studies of the vectors and reservoirs of this bacterium in Africa. Twenty-nine cat fleas, Ctenocephalides felis, were tested for the presence of rickettsiae, including R. felis, bartonellae, and borreliae, with specific quantitative real-time PCR assays. Supporting our previous studies, R. felis was not detected in the fleas collected. In addition, neither Bartonella nor Borrelia was found. In five (17% examined fleas, we found another species of rickettsia. We isolated three rickettsial strains, and genetic analysis demonstrated that these strains represent a probable new species, provisionally called Candidatus Rickettsia senegalensis here.

  17. Avaliação do jundiá (Rhamdia quelen em diferentes sistemas de cultivo para a região do litoral centro norte de Santa Catarina, Brazil

    Directory of Open Access Journals (Sweden)

    Hilton Amaral Junior

    2008-12-01

    Full Text Available El bagre (Rhamdia quelen, un pez nativo habitante de la mayoría de los ríos, lagos y lagunas de Santa Catarina, y que pertenecen a la orden Siluriformes, familia Pimelodidae, género Rhandia. El estudio tiene como objetivo evaluar el desempeño de este pez nativo en los sistemas más utilizados en Santa Catarina. El experimento se llevó a cabo en el ámbito del Campo Experimental de Piscicultura de Camboriú CEPC-EPAGRI, cuando 11 tanques excavados fueron utilizados, tres en representación de un monocultivo de bagre con una densidad de 1,5 p/m2, tres en representación de un bicultivo de bagre y tilapia con una densidad de 3p/m2, tres en representación de un policultivo de la carpa, tilapia y "jundiás" con una densidad de 3p/m2, y en dos tanques excavados fueron instalados seis tanques redes, en representación de las culturas de alta densidad de 50 y 100 p/m3.

  18. Tõsõ mu maailman : [luuletused] / Häniläne, pseud.

    Index Scriptorium Estoniae

    Häniläne,, pseud.

    2008-01-01

    Sisu: Bombus lucorum (maakimalane) ; Podiceps auritus (sarvikpütt) ; Malus domestica (aed-õunapuu) ; Felis catus domesticus (kodukass) ; Inachis io (päevapaabusilm) ; Lacerta vivipara (arusisalik) ; Canis familiaris (kodukoer) ; Clangula hyemalis (aul)

  19. Mercury contamination in Florida panthers [Draft

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — As a result of the death of an apparently healthy, radio-collared female Florida panther (Felis concolor qoryi) (FP#27) in Everglades National Park in July 1989, we...

  20. Phthiraptera from some wild carnivores in Spain

    OpenAIRE

    1990-01-01

    During 1987 and the first months of 1988, several carnivores were surveyed for ecological studies by means of radio-tracking techniques and in order to identify the ischnoceran species parasitising these animals. The hosts belonged to the following species: Felis pardina, Felis silvestris, Herpestes ichneumon, Genetta genetta, Vulpes vulpes and Metes meles. While no lice were found on the two first species, the remaining ones were parasitised by Felicola (Felicola) inaequalis, Lorisicol...

  1. Detection of Bartonellaspp. and Rickettsiaspp. in fleas, ticks and lice collected in rural areas of Peru

    Directory of Open Access Journals (Sweden)

    Abraham G. Cáceres

    2013-12-01

    Full Text Available Bartonellosis and rickettsiosis are commonly reported in Peru. In order to detect Bartonella sp. and Rickettsiasp. in fleas, ticks and lice, specimens from five distinct locations in Peru (Marizagua, Cajaruro, Jamalca, Lonya Grande and El Milagro were collected and screened for the presence of these bacteria using PCR and later confirmation by DNA sequencing. The specimens collected were distributed in 102 pools (76 Ctenocephalides felis, 2 Ctenocephalides canis, 16 Pulex irritans, 5 Pediculus humanus, 2 Rhiphicephalus sanguineus, and 1 Boophilus spp., where Bartonellawas detected in 17 pools (6 of C. felis, 9 of P. irritans, 1 of C. canis, and 1 P. humanus. Also, Rickettsiawas detected in 76 pools (62 C. felis, 10 P. irritans, 2 P. humanus, and 2 C. canis. Bartonella clarridgeiaewas detected in C. felis, C. canisand P. irritanspools at 5.3%, 50% and 12.5%, respectively.Bartonella rochalimaewas detected in one C. felisand two P. irritanspools at 1.3% and 12.5%, respectively. Furthermore, B. henselaewas detected in one C. felispool and one P. humanuspool corresponding to 1.3% and 20%, respectively; and Bartonella spp.was also found in 5 pools of P. irritansat 31.3%. Additionally, R. feliswas detected in C. felis, C. canisand P. irritanspools at 76.3%, 100% and 37.5%, respectively; and Rickettsia spp. was detected in C. felis, P. irritansand P. humanuspools at 5.3%, 25% and 40%, respectively. These results demonstrate the circulation of these bacteria in Peru.

  2. Differential Rickettsial Transcription in Bloodfeeding and Non-Bloodfeeding Arthropod Hosts

    Science.gov (United States)

    Verhoeve, Victoria I.; Jirakanwisal, Krit; Utsuki, Tadanobu; Macaluso, Kevin R.

    2016-01-01

    Crucial factors influencing the epidemiology of Rickettsia felis rickettsiosis include pathogenesis and transmission. Detection of R. felis DNA in a number of arthropod species has been reported, with characterized isolates, R. felis strain LSU and strain LSU-Lb, generated from the cat flea, Ctenocephalides felis, and the non-hematophagous booklouse, Liposcelis bostrychophila, respectively. While it is realized that strain influence on host biology varies, the rickettsial response to these distinct host environments remained undefined. To identify a panel of potential rickettsial transmission determinants in the cat flea, the transcriptional profile for these two strains of R. felis were compared in their arthropod hosts using RNAseq. Rickettsial genes with increased transcription in the flea as compared to the booklouse were identified. Genes previously associated with bacterial virulence including LPS biosynthesis, Type IV secretion system, ABC transporters, and a toxin-antitoxin system were selected for further study. Transcription of putative virulence-associated genes was determined in a flea infection bioassay for both strains of R. felis. A host-dependent transcriptional profile during bloodfeeding, specifically, an increased expression of selected transcripts in newly infected cat fleas and flea feces was detected when compared to arthropod cell culture and incubation in vertebrate blood. Together, these studies have identified novel, host-dependent rickettsial factors that likely contribute to successful horizontal transmission by bloodfeeding arthropods. PMID:27662479

  3. Utilização experimental de hidroxiapatita sintética em alvéolos dentários de gatos domésticos (Felis catus: estudo clínico, radiográfico e histomorfométrico

    Directory of Open Access Journals (Sweden)

    E.C. Silva

    2012-08-01

    Full Text Available A fim de avaliar a resposta biológica da hidroxiapatita sintética (HAP-91 nos alvéolos de felinos domésticos, este biomaterial foi implantado após extração do terceiro pré-molar inferior direito em 12 gatos e mantida por meio de uma membrana de celulose bacteriana. No lado esquerdo, os alvéolos foram apenas recobertos com a membrana de celulose bacteriana, formando o grupo-controle. Observou-se, durante a avaliação clínica, que todos os animais voltaram a comer normalmente ração úmida, sem apresentarem sinais de dor ou desconforto após a recuperação anestésica. A cicatrização da ferida cirúrgica ocorreu de forma satisfatória, sendo que a membrana de celulose bacteriana evitou a saída precoce da hidroxiapatita. Radiograficamente, aos 50 dias, todos os animais apresentaram radiopacidade óssea homogênea em ambos os lados. À análise histomorfométrica, observou-se adiantamento do processo de reparo do osso alveolar nos oito primeiros dias do grupo-tratado quando comparado ao grupo-controle, bem como atraso aos 30 dias, porém, aos 50 dias, ambos os grupos apresentavam porcentagem de tecido ósseo semelhante e morfologicamente normal. Os resultados sugerem que a hidroxiapatita é biocompatível, integra-se ao tecido ósseo alveolar e pode ser utilizada em felinos.

  4. Mammalian genome projects reveal new growth hormone (GH) sequences. Characterization of the GH-encoding genes of armadillo (Dasypus novemcinctus), hedgehog (Erinaceus europaeus), bat (Myotis lucifugus), hyrax (Procavia capensis), shrew (Sorex araneus), ground squirrel (Spermophilus tridecemlineatus), elephant (Loxodonta africana), cat (Felis catus) and opossum (Monodelphis domestica).

    Science.gov (United States)

    Wallis, Michael

    2008-01-15

    Mammalian growth hormone (GH) sequences have been shown previously to display episodic evolution: the sequence is generally strongly conserved but on at least two occasions during mammalian evolution (on lineages leading to higher primates and ruminants) bursts of rapid evolution occurred. However, the number of mammalian orders studied previously has been relatively limited, and the availability of sequence data via mammalian genome projects provides the potential for extending the range of GH gene sequences examined. Complete or nearly complete GH gene sequences for six mammalian species for which no data were previously available have been extracted from the genome databases-Dasypus novemcinctus (nine-banded armadillo), Erinaceus europaeus (western European hedgehog), Myotis lucifugus (little brown bat), Procavia capensis (cape rock hyrax), Sorex araneus (European shrew), Spermophilus tridecemlineatus (13-lined ground squirrel). In addition incomplete data for several other species have been extended. Examination of the data in detail and comparison with previously available sequences has allowed assessment of the reliability of deduced sequences. Several of the new sequences differ substantially from the consensus sequence previously determined for eutherian GHs, indicating greater variability than previously recognised, and confirming the episodic pattern of evolution. The episodic pattern is not seen for signal sequences, 5' upstream sequence or synonymous substitutions-it is specific to the mature protein sequence, suggesting that it relates to the hormonal function. The substitutions accumulated during the course of GH evolution have occurred mainly on the side of the hormone facing away from the receptor, in a non-random fashion, and it is suggested that this may reflect interaction of the receptor-bound hormone with other proteins or small ligands.

  5. 猫蚤的交配习性及雄蚤对雌蚤提取物的反应%MATING BEHAVIOR OF THE CAT FLEA, CTENOCEPHALIDES FELIS BOUCHE ( SIPHONAPTERA: PULICIDAE) AND MALE RESPONSE TO FEMALE EXTRACT ON AN ARTIFICIAL FEEDING SYSTEM

    Institute of Scientific and Technical Information of China (English)

    岳碧松; 邹方东; 孙奇志; 李静

    2002-01-01

    The mating behavior of cat flea, Ctenocephalidesfelis (Bouche) was studied on an artificial feeding de-.vice. Male and female can mate repeatedly with same partner or different ones. In the situation of male: female ratio of1: 5, each mating lasted an average of 6.6 min, with a mean interval between matings at 2.5 min., compared to 11.1 min and 12.1 min respectively in a cell with 5 males and 1 female. As many as 48 mating events were observed forone male during.an 8 h period. One female mated 27 times in 7 h with 5 males in the same cell. Newly emerged malesand females can not mate before blood meal and about 24 h blood feeding is required for successful mating. Newlyemerged males can not mate with fed females (fed for 48 h), but fed males can mate with newly emerged females whoare feeding the blood. Significantly more male contacts and male-male mating attempts were observed after the papertreated with female extract was introduced into the cell. The paper contacts and mating attempts were 16.75 - 32.25times and 15.75 -31.38 times, respectively, on average during a period of 20 min when different doses (FE) of ex-tract were provided.%在人工饲喂系统上研究了猫蚤的交配习性及雄蚤对雌蚤化学提取物的发应.结果表明,当5雌1雄在饲养盒内时,该雄虫可与其他雌虫进行多次交配,连续8小时内交配达48次,交配时间平均持续6.6分钟,两次交配的间隔时间平均为2.5分钟.当1雌5雄时,交配时间平均持续11.1分钟,交配间隔时间为12.1分钟,连续7小时内,该雌虫与雄虫交配27次.新羽化的雌雄虫吸血前不能交配.当把用雌虫提取物处理过的黑色滤纸片放进只有雄虫的饲养盒时,雄虫接触纸片的次数及雄-雄交配企图明显增加.

  6. MAST CELLS, MAST/STEM CELL GROWTH FACTOR RECEPTOR (C-KIT/CD117 AND IGE MAY BE INTEGRAL TO THE PATHOGENESIS OF ENDEMIC PEMPHIGUS FOLIACEUS

    Directory of Open Access Journals (Sweden)

    Ana Maria Roselino

    2013-11-01

    Full Text Available Introduction: Pemphigus foliaceus (PF is endemic in some South American countries, especially in Colombia and Brazil; in Brazil, it is also known as fogo selvagem (FS. We aimed to study the presence of mast cells and the expression of the mast/stem cell growth factor receptor (c-kit/CD117 in PF skin biopsies, as well as the role of IgE in the disease pathogenesis. Methods: Forty-four skin biopsies from patients affected by endemic PF (EPF (30 patients from El Bagre, Colombia, and 14 from the northeastern region of São Paulo State, Brazil, 48 control biopsies from Colombian and Brazilian endemic areas, and additional control biopsies from none endemic areas in Colombia and the USA non were studied. Immunohistochemistry (IHC was performed to evaluate skin biopsies with anti-mast cell tryptase (MCT, anti-c-kit and anti-IgE antibodies. We also searched for serum IgE in 30 EPF and 30 non-atopic controls from the El Bagre region via ELISA. In our El Bagre patients and controls, we also searched for IgE in skin samples by direct immunofluorescence. Results: In 100% of the EPF biopsies, MCT, c-kit and IgE were identified with stronger expression relative to control biopsies, especially in the inflammatory infiltrates around upper dermal blood vessels and dermal eccrine glands. IgE staining was positive along the BMZ in some EPF skin samples. The DIF results confirmed a linear deposition of IgE at the BMZ. Increased IgE serum levels were also noted in PF patients relative to controls.. Conclusions: In patients with EPF, the observed increased expression of MCT, c-kit and IgE in lesional skin, associated with higher serum IgE levels may indicate possible IgE participation in the antigenic response.

  7. Relevamiento íctico con espineles en el río Alto Iguazú (Misiones, Argentina)

    OpenAIRE

    Gómez, Sergio Enrique

    2014-01-01

    Este trabajo tiene como objetivo describir al arte de pesca denominado “espinel” que utilizamos en el alto Iguazú durante las décadas de los 80 y los 90, en un plan de relevamiento científico, y que permitió recolectar especies raras o nuevas para la Argentina (Gómez y Somay, 1985) como por ejemplo el bagre exótico Ictalurus nebulosus (Gómez, 2008). Se describen los espineles utilizados en el Alto Iguazú y la frecuencia o abundancia de las principales especies capturadas con este arte de pesc...

  8. APORTACIÓN A LA SEGURIDAD ALIMENTARIA HUMANA POR LA ACTIVIDAD PESQUERA EN EL LAGO DE ZAPOTLÁN Y LA PRESA BASILIO VADILLO

    OpenAIRE

    J. Guadalupe Michel Parra; Isabel Montaño Larios; Evangelina Díaz Andrade

    2012-01-01

    El presente estudio ecológico, tuvo como objetivo conocer las aportación productivas y nutrimentales de la actividad pesquera del lago de Zapotlán y la presa Basilio Vadillo, a la seguridad alimentaria; las especies ictiológicas alimentarias en común de los humedales son; tilapia, carpa y lobina. El charal y el bagre son la diferencia entre ambos. La captura mínima por pescador en la Laguna de Zapotlán es de 12.56 kg/día, y en la presa Basilio Vadillo es de 20 kg/día. El esfuerzo pesquero ...

  9. Two new species in the genus Cucullanus (Nematoda - Cucullanidae from the australian region

    Directory of Open Access Journals (Sweden)

    A. J. Petter

    1992-01-01

    Full Text Available Two new species of Cucullanus are described: C. bourdini n. sp. and C. laurotravassosi n. sp. C. bourdini is a parasite of Pristipomoides filamentosus , Aprion virescens and P. flavipinnis (Lutjanidae in New Caledonia. The species is closely related to C. amadai, C. bulbosus and C. hians by the disposition of the post-and ad-cloacal papille but differs by the more posterior position of the deirids. C. laurotravassosi n. sp., a parasite of Arius sp. in Australia, is close to C. bagre but can be distinguished by the more posterior position of post-cloacal papillae.

  10. Two new species in the genus Cucullanus (Nematoda - Cucullanidae) from the australian region

    OpenAIRE

    Petter,A. J.; Le Bel,J.

    1992-01-01

    Two new species of Cucullanus are described: C. bourdini n. sp. and C. laurotravassosi n. sp. C. bourdini is a parasite of Pristipomoides filamentosus , Aprion virescens and P. flavipinnis (Lutjanidae) in New Caledonia. The species is closely related to C. amadai, C. bulbosus and C. hians by the disposition of the post-and ad-cloacal papille but differs by the more posterior position of the deirids. C. laurotravassosi n. sp., a parasite of Arius sp. in Australia, is close to C. bagre but can ...

  11. Modelling growth-competition relationships in trembling aspen and white spruce mixed boreal forests of Western Canada.

    Directory of Open Access Journals (Sweden)

    Jian-Guo Huang

    Full Text Available We examined the effect of competition on stem growth of Picea glauca and Populus tremuloides in boreal mixedwood stands during the stem exclusion stage. We combined traditional approaches of collecting competition data with dendrochronology to provide retrospective measurements of stem diameter growth. Several competition indices including stand basal area (BA, the sum of stem diameter at breast height (SDBH, and density (N for the broadleaf and coniferous species, as well as similar indices considering only trees with diameters greater than each subject (BAGR, SDBHGR, and NGR, were evaluated. We used a nonlinear mixed model to characterize the basal area increment over the past 5, 10, 15, 20, 25, 30, and 35 years as a function of growth of nearby dominant trees, the size of the subject trees, deciduous and coniferous competition indices, and ecoregions. SDBHGR and BAGR were better predictors for spruce, and SDBHGR and NGR were better for aspen, respectively, than other indices. Results showed strongest correlations with long-term stem growth, as the best models integrated growth for 10-25 years for aspen and ≥ 25 for spruce. Our model demonstrated a remarkable capability (adjusted R(2>0.67 to represent this complex variation in growth as a function of site, size and competition.

  12. Modelling growth-competition relationships in trembling aspen and white spruce mixed boreal forests of Western Canada.

    Science.gov (United States)

    Huang, Jian-Guo; Stadt, Kenneth J; Dawson, Andria; Comeau, Philip G

    2013-01-01

    We examined the effect of competition on stem growth of Picea glauca and Populus tremuloides in boreal mixedwood stands during the stem exclusion stage. We combined traditional approaches of collecting competition data with dendrochronology to provide retrospective measurements of stem diameter growth. Several competition indices including stand basal area (BA), the sum of stem diameter at breast height (SDBH), and density (N) for the broadleaf and coniferous species, as well as similar indices considering only trees with diameters greater than each subject (BAGR, SDBHGR, and NGR), were evaluated. We used a nonlinear mixed model to characterize the basal area increment over the past 5, 10, 15, 20, 25, 30, and 35 years as a function of growth of nearby dominant trees, the size of the subject trees, deciduous and coniferous competition indices, and ecoregions. SDBHGR and BAGR were better predictors for spruce, and SDBHGR and NGR were better for aspen, respectively, than other indices. Results showed strongest correlations with long-term stem growth, as the best models integrated growth for 10-25 years for aspen and ≥ 25 for spruce. Our model demonstrated a remarkable capability (adjusted R(2)>0.67) to represent this complex variation in growth as a function of site, size and competition.

  13. Ecological knowledge and incidental capture of sea turtles in São João de Pirabas, Pará, Brazil

    Directory of Open Access Journals (Sweden)

    Tiago Pereira Brito

    2015-09-01

    Full Text Available This study aimed to register the ecological knowledge of fishermen from the municipality of São João de Pirabas, Pará, Brazil, regarding the occurrence of sea turtles on the Pará state coast, as well as measure their incidental capture when fishing; to do this, 50 semi-structured interviews were conducted with local fishermen. Fishing was practiced mostly by adult men, who used 7 fishing arts (gillnetting, hook and line, longline, fish corrals, net of tide canals, casting net, and basket trap, mainly aimed at catching king mackerel (Scomberomorus cavalla, serra Spanish mackerel (S. brasiliensis, king weakfish (Macrodon ancylodon, weakfish (Cynoscion spp., hake (Cynoscion spp., catfish (Bagre bagre, and mullet (Mugil spp.. Fishermen observed in the region 5 turtle species, with a more frequent occurrence of Chelonia mydas (100%, Dermochelys coriacea (66%, and Eretmochelys imbricata (46%; the less frequent species are Caretta caretta (16% and Lepidochelys olivacea (8%. The spawning areas of the 3 most common species demonstrate the significance of the Pará state coast for their conservation. Incidental capture was reported by 76% of fishermen, mainly occurring in net, longlines, and fish corrals. Usually, captured animals were released, although there is consumption of sea turtle meat and eggs by fishermen.

  14. Isolation and identification of Helicobacter spp, from canine and feline gastric mucosa

    DEFF Research Database (Denmark)

    Jalava, K.; On, Stephen L.W.; VanDamme, P.A.R.

    1998-01-01

    It is known that virtually all healthy adult dogs and cats harbor spiral helicobacters in their gastric mucosa, Three species, Helicobacter felis, Helicobacter bizzozeronii, and Helicobacter salomonis have been isolated in vitro from the gastric mucosa of these animals. The aims of this study were...... conventional phenotypic tests, whole-cell protein profiling, and ultrastructural analysis in identifying the different species isolated from canine and feline gastric mucose. We cultured 95 and 22 gastric mucosal biopsies from dogs and cats, respectively. Twenty-one H. bizzozeronii strains, 8 H. felis strains......, 8 H. salomonis strains, 3 mixed cultures, 2 "Flexispira rappini"-like organisms, and 3 as get uncharacterized strains were isolated from the dogs, and 3 H. felis strains were isolated from the cats. The methods used here yielded Helicobacter isolation rates of 51% from dogs and 13.6% from cats...

  15. Parasites of domestic and wild animals in South Africa. XLVIII . Ticks (Acari: Ixodidae infesting domestic cats and wild felids in southern Africa

    Directory of Open Access Journals (Sweden)

    Ivan G. Horak

    2010-08-01

    Full Text Available Ticks collected from domestic cats (Felis catus, cheetahs (Acinonyx jubatus,caracals (Caracal caracal, African wild cats (Felis lybica, black-footed cats (Felis nigripes, a serval (Leptailurus serval, lions(Panthera leo, and leopards (Panthera pardus were identified and counted. Thirteen species of ixodid ticks and one argasid tick were identified from domestic cats and 17 species of ixodid ticks from wild felids. The domestic cats and wild felids harboured 11 ixodid species in common. The adults of Haemaphysalis elliptica, the most abundant tick species infesting cats and wild felids, were most numerous on a domestic cat in late winter and in mid-summer, during 2 consecutive years. The recorded geographic distribution of the recently described Haemaphysalis colesbergensis, a parasite of cats and caracals, was extended by 2 new locality records in the Northern Cape Province,South Africa.

  16. Increasing frequency of feline cytauxzoonosis cases diagnosed in western Kentucky from 2001 to 2011.

    Science.gov (United States)

    Miller, Jean; Davis, Cheryl D

    2013-11-15

    Feline cytauxzoonosis is a rapidly progressing and usually fatal disease in domestic cats caused by the tick-borne pathogen, Cytauxzoon felis. The primary reservoir host for this protozoan parasite is the bobcat (Lynx rufus). In this retrospective study, we have examined the positive cases of feline cytauxzoonosis identified at Murray State University's Breathitt Veterinary Center, a regional diagnostic facility located in Hopkinsville, Kentucky, between January 2001 and December 2011. Center records reveal that there has been an increase in the rate of diagnosis of domestic feline infection with C. felis over that 10-year span with the majority of cases (75%) occurring between 2006 and 2011. The infection was diagnosed from March through October and showed a single peak in May, corresponding well with the questing period for the lone star tick, Amblyomma americanum, a known vector of C. felis.

  17. Bone marrow-derived cells may not be the original cells for carcinogen-induced mouse gastrointestinal carcinomas.

    Directory of Open Access Journals (Sweden)

    Chen Yang

    Full Text Available AIM: It has been reported that bone marrow-derived cells (BMDC can be original cells of mouse gastric cancers induced by Helicobacter felis (H. felis infection. However, it is unknown whether BMDCs are also the original cells of mouse gastrointestinal cancers induced by gastric carcinogens N-nitroso-N-methylurea (NMU and H. felis infection. METHODS: C57BL/6 recipient mice were initially irradiated with 10Gy X-ray, reconstituted with bone marrow cells from the C57BL/6-Tg (CAG-EGFP donor mice to label BMDCs with green fluorescence protein (GFP. After 4 weeks of recovery, the bone marrow-transplanted mice were given NMU in drinking water (240 ppm and subsequently infected with H. felis by gavage. Eighty weeks later, all mice were euthanized for pathological examination. The BMDCs expressing GFP were detected in tissues using direct GFP fluorescence confocal microscopy analysis and immunohistochemistry staining (IHC assays. RESULTS: Neoplastic lesions were induced by NMU treatment and/or H. felis infection at the antrum of the glandular stomach and small intestine. In the direct GFP fluorescence confocal assay, GFP(+ epithelial cell cluster or glands were not observed in these gastrointestinal tumors, however, most GFP(+ BMDCs sporadically located in the tumor stromal tissues. Some of these GFP(+ stromal BMDCs co-expressed the hematopoietic marker CD45 or myofibroblasts markers αSMA and SRF. In the indirect GFP IHC assay, similar results were observed among 11 gastric intraepithelial neoplasia lesions and 2 small intestine tumors. CONCLUSION: These results demonstrated that BMDCs might not be the source of gastrointestinal tumor cells induced by NMU and/or H. felis infection.

  18. A hypothesis linking sodium and lithium reabsorption in the distal nephron

    DEFF Research Database (Denmark)

    Thomsen, Klaus; Shirley, DG

    2006-01-01

      A hypothesis is proposed linking Na(+) and Li(+) reabsorption in the distal nephron. The handling of these two ions in the distal nephron is related because they share the same apical membrane entry mechanism: the amiloride-sensitive Na(+) channel (ENaC). However, the two ions exit the cell...... to the early distal tubule (FE(Li dist)), amounts to approximately 0.97. In contrast, during severe dietary Na(+) restriction, FE(Li dist) decreases to 0.50-0.60. Our hypothesis is that the absence of distal Li(+) reabsorption during intake of a normal diet can be explained by a negative driving force for Li...

  19. [Fleas (Siphonaptera) in the human environment. Analytic findings between 1961-1983 in the district of Leipzig (East Germany). II. Spacial and temporal distribution].

    Science.gov (United States)

    Vater, G; Vater, A

    1985-03-01

    During the last 20 years there has been very little infestation with fleas in the district of Leipzig. Out of the 8 species only Pulex irritans and Ctenocephalides felis are of hygienic concern. Greater numbers of them were found in densely populated urban districts. Origins of infestation were mainly dogs and cats kept in unhygienic conditions, and retreats of feral cats. There have been changes in the dominance of fleas associated with man. P. irritans probably had been the dominant species for centuries. About the turn of the 19th century Ctenocephalides canis attained codominance. Since the 1960s C. felis has been dominating.

  20. Ancylostoma genettae, A. protelesis, A. somaliense: three new species from wild Carnivora in the Somali Republic.

    Science.gov (United States)

    Macchioni, G

    1995-12-01

    Ancylostoma braziliense was found in Somalia in Acinonyx jubatus, Canis familiaris, C. mesomelas, Crocuta crocuta, Felis catus, F. libyca, Genetta genetta, Otocyon megalotis, Proteles cristatus; A. caninum in A. jubatus, C. familiaris, C. mesomelas, C. crocuta; A. duodenale in C. crocuta; A. iperodontatum in Lynx caracal; A. paraduodenale in Felis serval; A. tubaeforme in A. jubatus, F. catus, F. libyca; Arthrocephalus gambiense in Ichneumia albicauda; Uncinaria parvibursata in Mellivora capensis. In addition, three new species of Ancylostoma were collected: A. genettae in Genetta genetta, A. protelesis in Proteles cristatus, A. somaliense in Canis mesomelas. These new species are described and illustrated.

  1. Modelos emergentes en los sistemas y relaciones de género: nuevas socializaciones y políticas de implementación

    OpenAIRE

    Etxeberría, Feli

    2015-01-01

    APORTACIONES:Modelos emergentes en los sistemas y relaciones de género: nuevas socializacionesy políticas de implementación. Feli Etxeberria Sagastume, Pakita Arbe Mateo, MªCarmen Diez Mintegui y José Miguel Apaolaza Beraza APORTACIONES:Modelos emergentes en los sistemas y relaciones de género: nuevas socializacionesy políticas de implementación. Feli Etxeberria Sagastume, Pakita Arbe Mateo, MªCarmen Diez Mintegui y José Mig...

  2. Malaria in the colombian regions of Uraba and Bajo Cauca, province of Antioquia: an overwiew to interpret the antimalarial therapeutic failure La malaria en Colombia, Antioquia y las zonas de Urabá y Bajo Cauca: panorama para interpretar la falla terapéutica antimalárica. Parte 2

    Directory of Open Access Journals (Sweden)

    Jaime Carmona Fonseca

    2004-01-01

    Full Text Available Problem: in the past 5 years we have studied the treatment response to antimalarials and their combinations in the Antioquia region (Turbo, Zaragoza and El Bagre municipalities. The interpretation and better understanding of the treatment outcome (adequate clinical response, early treatment failure or late treatment failure require further information since this response depends on host’s and parasite’s factors, independently of the characteristics of the antimalarial administered. Objectives: to define an adequate reference frame which allows for interpretation of results obtained during antimalarials treatment assessment studies. This would include demography, epidemiology, climatic and social-economic information about Colombia, Antioquia, the regions of Uraba, Bajo Cauca and the municipalities of Turbo and El Bagre. Methodology: research of secondary information (publications reports and internet resources, and some primary information (taken from Sistema de Vigilancia Epidemiológica de Antioquia (Sivigila of Dirección Seccional de Salud de Antioquia DSSA. Data about treatment response were primary information (produced by Grupo Malaria, Universidad de Antioquia, or obtained from other authors. With the gathered data, tables and graphs were produced and calculations were carried out using other variables. Results: the frequency of malaria is presented for Colombia during the period 1960-2002 and the results of the malaria eradication and control programs, and their failure, are described. Similarly, the frequency of malaria in Antioquia during 1959-2003 is discussed and presented in terms of incidence rates and parasite annual indexes (PAI. Non adjusted and adjusted rates and PAI were obtained, therefore adjusted rates are presented by exposed population, which is less of 20%. Adjusted results are 5-6 fold higher that non-adjusted values in Antioquia. However this is not true for the Uraba and Bajo Cauca regions, where 100% of the

  3. Assessment of PCR-DGGE for the identification of diverse Helicobacter species, and application to faecal samples from zoo animals to determine Helicobacter prevalence

    DEFF Research Database (Denmark)

    Abu Al-Soud, W.; Bennedsen, M.; On, Stephen L.W.;

    2003-01-01

    bilis and Helicobacter hepaticus in a Nile crocodile, Helicobacter cinaedi in a baboon and a red panda, and Helicobacter felis in a wolf and a Taiwan beauty snake. All of these PCR products (similar to400 bp) showed 100 % sequence similarity to 16S rDNA sequences of the mentioned species. These results...

  4. Estudios sobre Educacion, 2002 (Studies on Education, 2002).

    Science.gov (United States)

    Estudios sobre Educacion, 2002

    2002-01-01

    This scholarly journal deals with the study of education. Articles in this second issue are: "La Vertebracion Academica de la Formacion Inicial del Profesorado" (The Academic Backbone of the Initial Formation of Teachers) (Jose M. Esteve); "Deficiencia Visual en el Nino" (Visual Deficiency in the Young Child) (Feli Peralta;…

  5. Hepatozoonosis in cats : ABCD guidelines on prevention and management

    NARCIS (Netherlands)

    Lloret, Albert; Addie, Diane D; Boucraut-Baralon, Corine; Egberink, Herman; Frymus, Tadeusz; Gruffydd-Jones, Tim; Hartmann, Katrin; Horzinek, Marian C; Hosie, Margaret J; Lutz, Hans; Marsilio, Fulvio; Pennisi, Maria Grazia; Radford, Alan D; Thiry, Etienne; Truyen, Uwe; Möstl, Karin

    2015-01-01

    OVERVIEW: Hepatozoonosis of domestic cats has been reported in several countries, mainly as a subclinical infection. DISEASE AGENT: Infection has been described mostly in areas where canine infection is present and, in recent years, Hepatozoon felis has been identified as a distinct species by molec

  6. Einige Bemerkungen über den Kaplöwen, Panthera leo melanochaitus (Ch. H. Smith, 1842)

    NARCIS (Netherlands)

    Mazak, V.

    1960-01-01

    In seinen Katalogen der Sammlungen des Leidener Museums führt Jentink (1887, p. 81; 1892, p. 95) zwei Schädel und ein aufgestelltes Exemplar von Felis leo Linnaeus an, welche aus der Kap-Kolonie stammen. Leider sind die genauen Fundorte, die Fangdaten und die Sammler unbekannt, ebenso der Weg, auf d

  7. Teaching A-level Genetics: The Coat Colours of the Domestic Cat.

    Science.gov (United States)

    Talbot, D. J.; Talbot, C. D.

    1992-01-01

    The authors provide an introduction to the inheritance of coat colors in cats and suggest strategies designed to integrate the domestic cat (Felis domesticus or catus) into the teaching of genetics. Provides examples to illustrate dominance, recessiveness, epistasis, multiple allelism, environmental effect of phenotype, incomplete dominance,…

  8. HISTOPATHOLOGICAL EVIDENCE OF REGENERATION FOLLOWING HEPATOTOXIC EFFECTS OF THE CYANOTOXIN MICROCYSTIN-LR IN THE HARDHEAD CATFISH AND GULF KILLIFISH.

    Science.gov (United States)

    The cyanobacterial toxin, microcystin-LR (MC-LR), causes liver damage in several freshwater fish species. The damage appears to be acute and irreversible. In the present study, two estuarine species, hardhead catfish, Arius felis, and gulf killifish, Fundulus grandis, were inject...

  9. Photographic records of the Asiatic Wildcat from two states of India

    OpenAIRE

    Pande, A; A. Vasava; R. Solanki; C.M. Bipin; Y V Jhala

    2013-01-01

    We report here photographic evidence for the Asiatic Wildcat Felis silvestris ornata Gray, 1830 from Shahgarh Landscape, Jaisalmer, western Rajasthan and Nauradehi Wildlife Sanctuary, central Madhya Pradesh. This is also the first photographic record of the species in the wild from Madhya Pradesh.

  10. Photographic records of the Asiatic Wildcat from two states of India

    Directory of Open Access Journals (Sweden)

    A. Pande

    2013-12-01

    Full Text Available We report here photographic evidence for the Asiatic Wildcat Felis silvestris ornata Gray, 1830 from Shahgarh Landscape, Jaisalmer, western Rajasthan and Nauradehi Wildlife Sanctuary, central Madhya Pradesh. This is also the first photographic record of the species in the wild from Madhya Pradesh.

  11. Plasma cortisol levels in captive wild felines after chemical restraint

    Directory of Open Access Journals (Sweden)

    G.P. Nogueira

    1997-11-01

    Full Text Available Eight Panthera onca (Po, 13 Felis concolor (Fc, 7 Felis yagouaroundi (Fy, 7 Felis tigrina (Ft and 5 Felis pardalis (Fp specimens from São Paulo State zoos were used. All animals were restrained with darts containing 10 mg/kg ketamine and 1 mg/kg xylazine. Venous blood samples were collected as soon as possible (within 15-20 min and serum was frozen until the time for cortisol quantification. Cortisol was determined using a solid phase radioimmunoassay with an intra-assay coefficient of 8.51%. Data were analyzed statistically by the Kruskal-Wallis test, followed by Dunn's multiple comparisons test, and the one-sample t-test, with the level of significance set at P<0.05. Data are reported as means ± SEM. Cortisol levels differed among the captive felines: Po = 166 ± 33a, Fc = 670 ± 118b, Fy = 480 ± 83b, Ft = 237 ± 42ab, Fp = 97 ± 12a nmol/l (values followed by different superscript letters were significantly different (P<0.001. Since most of the veterinary procedures on these species involve chemical restraint, these results show the necessity of preventive measures in order to minimize the effect of restraint stress on more susceptible species

  12. Cultural Resources Intensive Survey and Testing of Mississippi River Levee Berms Crittenden and Desha Counties, Arkansas and Mississippi, Scott, Cape Girardeau and Pemiscot Counties Missouri. Item R-752 Lambethville; Crittenden County, Arkansas

    Science.gov (United States)

    1984-05-01

    also stated that three large felines occurred throughout the earlier United States. These included the puma (Felis concolour), Jaguar ( Panthera onca ...and the giant Jaguar ( Panthera atrox). By 15,000 B.P. the large megafauna had given way to that found during modern times. Historic Environment

  13. 76 FR 71379 - Florida Power & Light Company, Turkey Point, Units 3 and 4; Draft Environmental Assessment and...

    Science.gov (United States)

    2011-11-17

    ... supply. The FAS underlies approximately 100,000 square miles (258,000 km\\2\\) in southern Alabama..., mollusks, crustaceans, fish, sea turtles, and marine mammals. The portion of Biscayne Bay adjacent to... swallowtail E ponceanus. butterfly. Mammals Puma concolor mountain lion \\b\\... T/SA Felis concolor...

  14. Presence of Bartonella species in wild carnivores of northern Spain.

    Science.gov (United States)

    Gerrikagoitia, Xeider; Gil, Horacio; García-Esteban, Coral; Anda, Pedro; Juste, R A; Barral, Marta

    2012-02-01

    The genus Bartonella was detected by PCR in 5.7% (12/212) of wild carnivores from Northern Spain. Based on hybridization and sequence analyses, Bartonella henselae was identified in a wildcat (Felis silvestris), Bartonella rochalimae in a red fox (Vulpes vulpes) and in a wolf (Canis lupus), and Bartonella sp. in badgers (Meles meles).

  15. Presence of Bartonella Species in Wild Carnivores of Northern Spain

    OpenAIRE

    Gerrikagoitia, Xeider; Gil, Horacio; García-Esteban, Coral; Anda, Pedro; R.A. Juste; Barral, Marta

    2012-01-01

    The genus Bartonella was detected by PCR in 5.7% (12/212) of wild carnivores from Northern Spain. Based on hybridization and sequence analyses, Bartonella henselae was identified in a wildcat (Felis silvestris), Bartonella rochalimae in a red fox (Vulpes vulpes) and in a wolf (Canis lupus), and Bartonella sp. in badgers (Meles meles).

  16. Does hair coat length affect flea infestation in naturally infested dogs?

    Directory of Open Access Journals (Sweden)

    Guilherme Araujo Collares da Silva

    Full Text Available Abstract The Siphonaptera are parasitic insects of endothermic animals and Ctenocephalides felis and Pulex irritans are important parasites of dogs. This study evaluated the effect of hair coat length and time of year on the population size of C. felis and P. irritans in naturally infested dogs. Fleas were collected from 14 dogs on a monthly basis for a year (February 2015 to January 2016 at a rural property in Bagé, Rio Grande do Sul, Brazil. The dogs were divided into two groups based on hair coat length: short coat (coat length 5.0 cm, n= 7. In total, 2057 fleas were collected, 1541 of which were C. felis (74.91% and 516 were P. irritans (25.08%. The number of C. felis and P. irritans individuals was significantly affected by hair coat length and time of year. The variation in flea numbers over the study months was higher in long-coated than in short-coated dogs for the two flea species and flea numbers increased with increasing mean monthly temperatures. The results provide a better understanding of behavioral aspects of flea communities in dogs and may help develop control strategies targeting these parasites.

  17. Rickettsia infection in five areas of the state of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Maurício C Horta

    2007-11-01

    Full Text Available This study investigated rickettsial infection in animals, humans, ticks, and fleas collected in five areas of the state of São Paulo. Eight flea species (Adoratopsylla antiquorum antiquorum, Ctenocephalides felis felis, Polygenis atopus, Polygenis rimatus, Polygenis roberti roberti, Polygenis tripus, Rhopalopsyllus lugubris, and Rhopalopsyllus lutzi lutzi, and five tick species (Amblyomma aureolatum, Amblyomma cajennense, Amblyomma dubitatum, Ixodes loricatus, and Rhipicephalus sanguineus were collected from dogs, cats, and opossums. Rickettsia felis was the only rickettsia found infecting fleas, whereas Rickettsia bellii was the only agent infecting ticks, but no animal or human blood was shown to contain rickettsial DNA. Testing animal and human sera by indirect immunofluorescence assay against four rickettsia antigens (R. rickettsii, R. parkeri, R. felis, and R. bellii, some opossum, dog, horse, and human sera reacted to R. rickettsii with titers at least four-fold higher than to the other three rickettsial antigens. These sera were considered to have a predominant antibody response to R. rickettsii. Using the same criteria, opossum, dog, and horse sera showed predominant antibody response to R. parkeri or a very closely related genotype. Our serological results suggest that both R. rickettsii and R. parkeri infected animals and/or humans in the studied areas.

  18. Environmental Assessment Newport Research Facility Irish Hill

    Science.gov (United States)

    2011-09-12

    pasture to that has been recently seeded as a hay field. The northern portion of this parcel is primarily a moderately to steeply sloped forested...Name ANIMALS American burying beetle Canada lynx Eastern cougar Gray wolf Scientific Name Nicrophorus americanus Lynx canadensis Puma (=Felis...concolor couguar Canis lupus Northeastern beach tiger beetle Cincindela dorsalis dorsalis PLANTS Northeastern bulrush Swamp pink Scirpus

  19. Increased vitamin D-driven signalling and expression of the vitamin D receptor, MSX2, and RANKL in tooth resorption in cats

    NARCIS (Netherlands)

    Booij-Vrieling, H.E.; Ferbus, D.; Tryfonidou, M.A.; Riemers, F.M.; Penning, L.C.; Berdal, A.; Everts, V.; Hazewinkel, H.A.W.

    2010-01-01

    Tooth resorption occurs in 20-75% of cats (Felis catus). The aetiology is not known, but vitamin D is suggested to be involved. Vitamin D acts through a nuclear receptor (VDR) and increases the expression of receptor activator of nuclear factor-κB ligand (rankl) and muscle segment homeobox 2 (msx2)

  20. Activity of Cathelicidin Peptides against Chlamydia spp.

    Science.gov (United States)

    Donati, Manuela; Di Leo, Korinne; Benincasa, Monica; Cavrini, Francesca; Accardo, Silvia; Moroni, Alessandra; Gennaro, Renato; Cevenini, Roberto

    2005-01-01

    The in vitro activity of six cathelicidin peptides against 25 strains of Chlamydia was investigated. SMAP-29 proved to be the most active peptide, reducing the inclusion numbers of all 10 strains of Chlamydia trachomatis tested by ≥50% at 10 μg/ml. This peptide was also active against C. pneumoniae and C. felis. PMID:15728927

  1. Notes on some ectoparasites received by the Medical Entomology Unit, Institute for Medical Research.

    Science.gov (United States)

    Lee, H L; Krishnasamy, M; Jeffery, J; Paramasvaran, S

    2006-06-01

    There were a spate of recent complaints of insect bites and the entomological specimens received from various sources were identified to be those of cat flea (Ctenocephalides felis) and rat flea (Xenopsylla cheopis), the tropical bed-bug (Cimex hemipterus) and the dog louse (Heterodoxus spiniger). Only the fleas and the bed-bug are known to attack humans.

  2. 21 CFR 520.2130 - Spinosad.

    Science.gov (United States)

    2010-04-01

    ... FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) ANIMAL DRUGS, FEEDS... in § 510.600 of this chapter. (c) Conditions of use in dogs—(1) Amount. Administer tablets once a... felis) on dogs for 1 month. (3) Limitations. Federal law restricts this drug to use by or on the...

  3. Crecimiento de alevines de Ictalurus Balsanus (pisces:ictaluridae) en condiciones de laboratorio.

    OpenAIRE

    Jorge Luna-Figueroa; Elsah Arce Uribe

    2016-01-01

    Se realizó un estudio para evaluar el efecto de tres dietas sobre el crecimiento de alevines de Ictalurus balsanus en condiciones de laboratorio. La talla inicial de los peces fue 39.68 ± 2.0 mm de longitud total y 411.47 ± 17.00 mg de peso corporal, colocados en grupos de 10 peces por acuario de 80L. Los bagres fueron alimentados con tres dietas diferentes: I(31,13% proteína, 2,70% grasa, 56,53% carbohidratos), II(39,12%, 3,0%, 35,0%), III(53,57%, 19,37%, 4,17%). Los resultados indican que e...

  4. IN SITU IMMUNE RESPONSE EVALUATION VIA IMMUNOHISTOCHEMISTRY IN SKIN BIOPSIES FROM PATIENTS AFFECTED BY AUTOIMMUNE BLISTERING DISEASES

    Directory of Open Access Journals (Sweden)

    Ana Maria Abreu Velez

    2013-11-01

    Full Text Available Introduction: The in situ immune response in skin biopsies from patients affected by autoimmune skin blistering diseases (ABD is not well characterized. Aim: Our investigation attempts to immunophenotype cells in lesional skin in several ABD, utilizing immunohistochemistry (ICH. Methods: We tested by IHC for CD4, CD8, CD19, CD20, CD45, CD56/NCAM, PAX-5, granzyme B, myeloperoxidase, neutrophil elastase, LAT and ZAP-70 in patients affected by ABD. We tested 30 patients with endemic pemphigus foliaceus (EPF, 15 controls from the EPF endemic area, and 15 biopsies from healthy controls from the USA. We also tested archival biopsies from patients with selected ABD, including 30 patients with bullous pemphigoid, 20 with pemphigus vulgaris, 8 with pemphigus foliaceus and 14 with dermatitis herpetiformis. Results: We found a predominantly CD8 positive/CD45 positive T cell infiltrate in all ABD. Our skin biopsies demonstrated consistently positive staining for myeloperoxidase, but negative staining for neutrophil elastase. Most ABD biopsies displayed negative staining for CD4 and B cell markers; natural killer cell markers were also rarely seen. ZAP-70 and LAT were frequently detected. In El Bagre-EPF, a significant fragmentation of T cells in lesional skin was noted, as well as autoreactivity to lymph nodes. Conclusions: The documented T cell and myeloperoxidase staining are indicative of the role of T lymphocytes and neutrophils in lesional biopsies in patients with ABD, in addition to previously documented deposition of B cells, immunoglobulins and complement in situ. In El Bagre-EPF, T cells could also target lymph nodes; however, further studies are needed to confirm this possibility.

  5. Malaria, desnutrición y parasitosis intestinal en los niños colombianos: interrelaciones interrrelations between malaria, malnutrition and intestinal parasitism in colombian children

    Directory of Open Access Journals (Sweden)

    Jaime Carmona Fonseca

    2004-09-01

    Full Text Available Este trabajo resume datos colombianos y los hallazgos del Grupo Malaria (Universidad de Antioquia sobre las relaciones entre malaria, desnutrición y funcionamiento inmunitario, trabajos realizados en niños (4-11 años de las poblaciones antioqueñas de Turbo, El Bagre y Zaragoza. Se hace énfasis en las relaciones malaria, parásitos intestinales y desnutrición (desnutrición crónica, hipovitaminosis A, las cuales se exploran a través de su articulación en el sistema inmunitario. Se formulan recomendaciones para la aplicación clínica (individual y epidemiológica (colectiva con respecto a la formulación de suplemento de vitamina A y antihelmínticos de amplio espectro. En Turbo y en El Bagre y Zaragoza: 1 la frecuencia de malaria registra índices parasitarios anuales de 39 (Turbo y 156 casos por cada mil sujetos expuestos (El Bagre-Zaragoza en 1996-2000; 2 el riesgo de desnutrición crónica (índice talla/ edad es de 63% en los niños de 3-11 años; 3 se encontró anemia en el 26% de los palúdicos y 17% de los no palúdicos; 4 el 65% de los niños con malaria y el 35% de aquellos sin malaria mostraron valores de retinol bajos (<0,3 µg/mL; 5 se hallaron niveles anormalmente bajos de apoproteína A-1, tanto en los niños sin malaria como, sobre todo, en los que sí la tenían; 6 el 96% de los niños (4-9 años con malaria mostraron títulos de interleuquina 10 (IL10 muy altos con relación a lo normal y estadísticamente superiores a los exhibidos por los niños sin malaria; 7 los títulos de IgE total y específica anti-Plasmodium estuvieron anormalmente altos en los niños de ambos municipios e igual sucedió con los niveles de FNT-; 8 en estudiantes y docentes de enfermería, aparentemente sanos, con edades de 18-44 años, hallamos parásitos intestinales en el 97% y parásitos intestinales patógenos en el 42%. En menores de 5 años de Turbo hay parásitos intestinales patógenos en un 30-35% de los niños, con predominio de G

  6. Ectoparasitos de cães e gatos da cidade de Manaus, Amazonas, Brasil Ectoparasites on cats and dogs from Manaus, Amazonas State, Brazil

    Directory of Open Access Journals (Sweden)

    Marcelo Cutrim Moreira de Castro

    2006-12-01

    Full Text Available São apresentados resultados da coleta de ectoparasitos em cães e gatos entre agosto de 2001 e maio de 2002 em diferentes bairros da cidade Manaus. No cão foram encontrados: Ctenocephalides f. felis (Bouché, 1835 (Siphonaptera, Pulicidae, Heterodoxus spiniger (Enderlein, 1909(Phthiraptera, Boopidae, Trichodectes canis (De Geer, 1778 (Phthiraptera, Trichodectidae e Rhipicephalus sanguineus (Latreille,1806 (Acari, Ixodidae. No gato foi coletado C. f. felis. A prevalência de ectoparasitos foi de 80,8% para cães e 72,7% para gatos. Para a pulga C. f. felis foi de 28,7% para cães e 72,7% para gatos. Para o piolho H. spiniger foi de 12,3% para cães. Para o piolho T. canis foi de 0,1% para cães e para o carrapato R. sanguineus foi de 63% para cães. A média de infestaçãode pulga foi de 1,26 para cães e 1,27 para gatos. A proporção sexual fêmea/macho foi de 1,96:1 no cão e de 3,66:1 no gato. A pulga C. canis (Curtis, 1826, registrada em 1922, não foi coletada.Ectoparasites from different neighborhood of Manaus were collected from august 2001 to May 2002. On dogs it was found: Ctenocephalides f. felis (Bouché, 1835 (Siphonaptera, Pulicidae, Heterodoxus spiniger (Enderlein, 1909(Phthiraptera, Boopidae, Trichodetes canis (De Geer, 1778 (Phthiraptera, Trichodectidae and Rhipicephalus sanguineus (Latreille,1806 (Acari, Ixodidae. On cats: C. f. felis. The prevalence of ectoparasites was 80.8% to dogs and 72.7% to cats. For the flea C. f. felis was 28.7% to dogs and 72.7% to cats. For the lice H. spiniger was 12.3% for dogs. For the lice T. canis was 0.1% for dogs and for the tick R. sanguineus was 63% for dogs. The infestation index for fleas was 1.26 to dogs and 1.27 to cats. The sexual ratio obtained was 1.96:1 to dogs and 3.66:1 to cats. The flea C. canis (Curtis, 1826 registered in 1922 was not found.

  7. Feline chlamydiosis.

    Science.gov (United States)

    Sykes, Jane E

    2005-05-01

    Chlamydiae are an important cause of acute and chronic conjunctivitis in cats. Until recently, only one organism was thought to infect cats, Chlamydophila felis (previously Chlamydia psittaci var. felis). Recently, other Chlamydia-like organisms belonging to the family Parachlamydiaceae, which comprises organisms that reside and proliferate within free-living amoeba, have been identified in cats with neutrophilic and eosinophilic conjunctivitis. The relative importance of these organisms and their amoebic hosts requires investigation. There is also weak evidence that chlamydiae may also be capable of causing reproductive tract disease and lameness in cats. Diagnosis of chlamydial conjunctivitis requires use of specialized culture techniques or the polymerase chain reaction. The antibiotic of choice to treat these infections is doxycycline; azithromycin is less effective. All cats in the household should be treated simultaneously. The zoonotic potential of these organisms appears low, but some precaution is warranted when handling affected cats.

  8. The Near Eastern origin of cat domestication.

    Science.gov (United States)

    Driscoll, Carlos A; Menotti-Raymond, Marilyn; Roca, Alfred L; Hupe, Karsten; Johnson, Warren E; Geffen, Eli; Harley, Eric H; Delibes, Miguel; Pontier, Dominique; Kitchener, Andrew C; Yamaguchi, Nobuyuki; O'brien, Stephen J; Macdonald, David W

    2007-07-27

    The world's domestic cats carry patterns of sequence variation in their genome that reflect a history of domestication and breed development. A genetic assessment of 979 domestic cats and their wild progenitors-Felis silvestris silvestris (European wildcat), F. s. lybica (Near Eastern wildcat), F. s. ornata (central Asian wildcat), F. s. cafra (southern African wildcat), and F. s. bieti (Chinese desert cat)-indicated that each wild group represents a distinctive subspecies of Felis silvestris. Further analysis revealed that cats were domesticated in the Near East, probably coincident with agricultural village development in the Fertile Crescent. Domestic cats derive from at least five founders from across this region, whose descendants were transported across the world by human assistance.

  9. Real-Time Detection and Identification of Chlamydophila Species in Veterinary Specimens by Using SYBR Green-Based PCR Assays

    DEFF Research Database (Denmark)

    Nordentoft, Steen; Kabell, Susanne; Pedersen, Karl

    2011-01-01

    of Chlamydiaceae and differentiate the most prevalent veterinary Chlamydophila species: Cp. psittaci, Cp. abortus, Cp. felis, and Cp. caviae. By adding bovine serum albumin to the master mixes, target DNA could be detected directly in crude lysates of enzymatically digested conjunctival or pharyngeal swabs...... or tissue specimens from heart, liver, and spleen without further purification. The assays were evaluated on veterinary specimens where all samples were screened using a family-specific PCR, and positive samples were further tested using species-specific PCRs. Cp. psittaci was detected in 47 birds, Cp....... felis was found in 10 cats, Cp. caviae was found in one guinea pig, and Cp. abortus was detected in one sheep. The screening assay appeared more sensitive than traditional microscopical examination of stained tissue smears. By combining a fast, robust, and cost-effective method for sample preparation...

  10. [Felines: an alternative in genetic toxicology studies?].

    Science.gov (United States)

    Zamora-Perez, Ana; Gómez-Meda, Belinda C; Ramos-Ibarra, Maria L; Batista-González, Cecilia M; Luna-Aguirre, Jaime; González-Rodríguez, Andrés; Rodríguez-Avila, José L; Zúñiga-González, Guillermo M

    2008-06-01

    The micronuclei (MN) test carry out in peripheral blood is fast, simple, economic and it is used to detect genotoxic environmental agents. MN are fragments of chromosomes or complete chromosomes remaining in the cytoplasm after cell division, which increase when organisms are exposed to genotoxic agents. Therefore, species with the highest values of spontaneous micronucleated erythrocytes (MNE) are the most suitable to be potentials biomonitor of micronucleogenic agents, using a drop of blood. Nine species of Felines that present spontaneous MNE in peripheral blood are shown. From these species, the cat has been previously proven, with positive results and also lion (Panthera leo), yaguaroundi (Felis yagoaroundi), lynx (Lynx ruffus), jaguar (Panthera onca), puma (Puma concolor), tiger (Panthera tigris), ocelote (Felis padalis) and leopard (Panthera pardus) display spontaneous MNE, and with this characteristic this Family can be propose like a potential group to be used in toxicogenetic studies.

  11. Parasites of domestic and wild canids in the region of Serra do Cipó National Park, Brazil Parasitos de canídeos domésticos e silvestres na região do Parque Nacional da Serra do Cipó - Brasil

    Directory of Open Access Journals (Sweden)

    Juliana Lúcia Costa Santos

    2012-09-01

    Full Text Available Over recent decades, diseases have been shown to be important causes of extinctions among wild species. Greater emphasis has been given to diseases transmitted by domestic animals, which have been increasing in numbers in natural areas, along with human populations. This study had the aim of investigating the presence of intestinal helminths in wild canids (maned wolf, Chrysocyon brachyurus, and crab-eating fox, Cerdocyon thous in the Serra do Cipó National Park (43-44º W and 19-20º S and endo and ectoparasites of domestic dogs in the Morro da Pedreira Environmental Protection Area (an area surrounding the National Park. The Serra do Cipó is located in the state of Minas Gerais, Brazil. Among the enteroparasites found in domestic and wild canids, the following taxons were identified: Ancylostomidae, Trichuridae, Toxocara sp., Spirocerca sp., Physaloptera sp., Strongyloides sp., Cestoda, Dipylidium caninum, Diphyllobothriidae, Hymenolepidae, Anoplocephalidae, Trematoda, Acanthocephala and Isospora sp. Domestic dogs were positive for leishmaniasis and Babesia canis in serological tests. Among the ectoparasites, Rhipicephalus sanguineus, Amblyomma cajennense and Ctenocephalides felis felis were observed in domestic dogs. Variations in the chaetotaxy of the meta-episternum and posterior tibia were observed in some specimens of C. felis felis.Nas últimas décadas, as doenças têm sido apontadas como importantes causas de extinção de espécies silvestres. Maior ênfase tem sido dada às doenças transmitidas por animais domésticos que crescem em número, bem como as populações humanas, em áreas naturais. O presente estudo objetivou verificar a presença de helmintos intestinais de canídeos silvestres (lobo-guará - Chrysocyon brachyurus e cachorro-do-mato - Cerdocyon thous do Parque Nacional da Serra do Cipó (43-44º W e 19-20º S e endo e ectoparasitos de cães domésticos da Área de Proteção Ambiental Morro da Pedreira (entorno do

  12. Faecal helminth egg and oocyst counts of a small population of African lions (Panthera leo in the southwestern Kalahari, Namibia : research communication

    Directory of Open Access Journals (Sweden)

    Y. Smith

    2006-09-01

    Full Text Available An endoparasite survey of a small pride of African lions (Panthera leo was conducted at Intu Afrika Kalahari Game Reserve, southwestern Namibia, during winter and summer of 2003 and 2004, respectively. Overall, 23 fresh lion scats were collected opportunistically during fieldwork trials. A flotation technique was employed for the diagnosis of parasites. Three nematodes, Ancylostoma braziliense, Gnathostoma spinigerum and Uncinaria stenocephala and two coccidians, Toxoplasma gondii and Isospora felis were recorded. By using the McMaster method for quantification, a maximum number of 14 866 oocysts per gram of faeces was obtained for I. felis during winter 2003. Endoparasite taxa carried by the different individuals in the pride were found to be related to their levels of association. Rates of infection were relatively low as a result of the habitat, semi-captive conditions and earlier sporadic deworming.

  13. Ectoparasites of small ruminants in three selected agro-ecological sites of Tigray Region, Ethiopia.

    Science.gov (United States)

    Mulugeta, Y; Yacob, Hailu T; Ashenafi, Hagos

    2010-08-01

    A study on ectoparasites of small ruminants in three selected agro-ecological sites of Tigray Region, Ethiopia disclosed an overall prevalence of 55.5% and 58% in each examined 750 sheep and goats, respectively. In the sheep population, Melophagus ovinus (19.1%), tick infestations (16%), Damalinia ovis (15.3%), Linognathus africanus (11.5%), and Ctenocephalides felis (9%) were the major ectoparasites. The major ectoparasites identified in goats were tick infestations (29.7%), L. africanus (27.9%), Sarcoptes scabiei var. caprae (12.5%), C. felis (11.1%), and Demodex caprae (6.8%). In sheep, there was a statistically significant difference (P consciousness and awareness of farmers, and weak animal health extension services are believed to have contributed for widespread distribution and occurrences of ectoparasites. The growing threat of ectoparasites to small ruminant production and the tanning industry needs well-coordinated and urgent control intervention.

  14. Erratum: ``Whittaker pairs for the Virasoro algebra and the Gaiotto-Bonelli-Maruyoshi-Tanzini states'' [J. Math. Phys. 53, 033504 (2012)

    Science.gov (United States)

    Felińska, Ewa; Jaskólski, Zbigniew; Kosztołowicz, Michał

    2012-12-01

    A flow in the formulation and proof of Lemma 2.7 of E. Felińska, Z. Jaskólski, and M. M. Kosztołowicz, J. Math. Phys. 53, 033504 (2012) is fixed in Sec. I of this Erratum. This has no consequences for the rest of the paper. An essential error was made in Theorems 3.5, 3.6, and Corollary 3.7 of Sec. III of E. Felińska, Z. Jaskólski, and M. M. Kosztołowicz, J. Math. Phys. 53, 033504 (2012). As it was pointed out by V. Mazorchuk and K. Zhao ["Simple Virasoro modules which are locally finite over positive part," e-print arXiv:1205.5937v2 [math.RT

  15. A Molecular survey of Anaplasma spp., Rickettsia spp., Ehrlichia canis and Babesia microti in foxes and fleas from Sicily.

    Science.gov (United States)

    Torina, A; Blanda, V; Antoci, F; Scimeca, S; D'Agostino, R; Scariano, E; Piazza, A; Galluzzo, P; Giudice, E; Caracappa, S

    2013-11-01

    Fleas (Insecta: Siphonaptera) are obligate bloodsucking insects, which parasitize birds and mammals, and are distributed throughout the world. Several species have been implicated in pathogen transmission. This study aimed to monitor red foxes and the fleas isolated from them in the Palermo and Ragusa provinces of Sicily, Italy, as these organisms are potential reservoirs and vectors of pathogens. Thirteen foxes (Vulpes vulpes) and 110 fleas were analysed by polymerase chain reaction (PCR) to detect DNA of the pathogens Ehrlichia canis, Babesia microti, Rickettsia spp., Anaplasma phagocytophilum, Anaplasma platys, Anaplasma marginale and Anaplasma ovis. In the foxes, A. ovis was detected in only one animal, whereas the prevalence of the E. canis pathogen was 31%. B. microti and Rickettsia spp. were not detected. Of all of the collected fleas, 75 belonged to the species Xenopsylla cheopis, 32 belonged to Ctenocephalides canis, two belonged to Ctenocephalides felis and one belonged to Cediopsylla inaequalis. In the fleas, the following pathogens were found: A. ovis (prevalence 25%), A. marginale (1%), A. phagocytophilum (1%), Rickettsia felis (2%) and E. canis (3%). X. cheopis was the flea species most frequently infected with Anaplasma, in particular A. ovis (33%), A. marginale (1%) and A. phagocytophilum (1%). Both C. felis exemplars were positive for R. felis. E. canis was found in the lone C. inaequalis and also in 3% of the X. cheopis specimens. No fleas were positive for B. microti or A. platys. As foxes often live in proximity to domestic areas, they may constitute potential reservoirs for human and animal parasites. Further studies should be performed on fleas to determine their vectorial capacity.

  16. Exposure to positively- and negatively-charged plasma cluster ions impairs IgE-binding capacity of indoor cat and fungal allergens

    OpenAIRE

    NISHIKAWA, Kazuo; Fujimura, Takashi; Ota, Yasuhiro; Abe, Takuya; ElRamlawy, Kareem Gamal; Nakano, Miyako; Takado, Tomoaki; Uenishi, Akira; Kawazoe, Hidechika; Sekoguchi, Yoshinori; Tanaka, Akihiko; Ono, Kazuhisa; Kawamoto, Seiji

    2016-01-01

    Background Environmental control to reduce the amount of allergens in a living place is thought to be important to avoid sensitization to airborne allergens. However, efficacy of environmental control on inactivation of airborne allergens is not fully investigated. We have previously reported that positively- and negatively-charged plasma cluster ions (PC-ions) reduce the IgE-binding capacity of crude allergens from Japanese cedar pollen as important seasonal airborne allergens. Cat (Felis do...

  17. Localization of Helicobacter spp. in the fundic mucosa of laboratory Beagle dogs: an ultrastructural study

    Directory of Open Access Journals (Sweden)

    Lanzoni Anna

    2011-03-01

    Full Text Available Abstract In dogs Helicobacter spp. are found in all gastric regions usually localized in the surface mucus, gastric glands and parietal cells. The aim of this study was to detail the distribution of Helicobacter spp. in the fundic mucosa of asymptomatic Beagle dogs and their intracellular localization within parietal cells, in order to evaluate species-specific pathogenetic effects on gastric cells. The presence of Helicobacter spp. was investigated by immunohistochemistry, TEM, and PCR in the fundic mucosa of six Beagle dogs. Helicobacter spp. were found in all dogs examined, and H. bizzozeronii and H. felis were identified by PCR and confirmed by TEM. In the lumen of the fundic glands, co-localization was common. H. bizzozeronii was present in larger numbers than H. felis in both intraluminal and intraparietal localization. The amounts of H. bizzozeronii were similar in superficial and basal portions of the glands. H. felis was predominantly localized in the superficial portions of gastric glands but almost absent from the base. Within parietal cells, most Helicobacter organisms were intracanalicular, but intact and degenerate Helicobacter organisms were also visualized free in the cytoplasm or in secondary lysosomes. No specific degenerative lesions were found in infected parietal cells. Helicobacter organisms were also observed within macrophages in the lamina propria. In conclusion, there is a differential distribution of H. bizzozeronii and H. felis in the fundic mucosa of Beagle dogs, and their intracellular localization in parietal cells and macrophages suggests novel pathogenic scenarios for the development of immune response and maintenance of chronic gastritis in dogs.

  18. Invertebrate vectors, parasites, and rickettsial agents in Guam

    Directory of Open Access Journals (Sweden)

    Johnson, J.

    2012-12-01

    Full Text Available We conducted a 3-week field study of ectoparasites of humans and domestic animals throughout Guam. Thirteen species of ectoparasitic arthropods were collected. Ectoparasites of medical or veterinary significance included the ticks Rhipicephalus sanguineus and Rhipicephalus microplus, fleas Ctenocephalides felis and Xenopsylla cheopsis, and the head louse Pediculus humanus capitis. Polymerase chain reaction based screening for rickettsial and protozoan pathogens detected pathogens in eight arthropods. These included Anaplasma platys, Coxiella burnetii, Babesia canis vogeli, and Hepatozoon canis.

  19. Transposon Insertion Reveals pRM, a Plasmid of Rickettsia monacensis▿

    OpenAIRE

    Baldridge, Gerald D.; Burkhardt, Nicole Y.; Felsheim, Roderick F.; Timothy J Kurtti; Munderloh, Ulrike G.

    2007-01-01

    Until the recent discovery of pRF in Rickettsia felis, the obligate intracellular bacteria of the genus Rickettsia (Rickettsiales: Rickettsiaceae) were thought not to possess plasmids. We describe pRM, a plasmid from Rickettsia monacensis, which was detected by pulsed-field gel electrophoresis and Southern blot analyses of DNA from two independent R. monacensis populations transformed by transposon-mediated insertion of coupled green fluorescent protein and chloramphenicol acetyltransferase m...

  20. 猫抓病的实验室检查、临床病理特征及治疗

    Institute of Scientific and Technical Information of China (English)

    谈顺

    2002-01-01

    @@ 猫抓病在国外较多见,随着豢养宠物人群不断扩大,国内报道近几年也逐渐多了起来.过去不少研究者认为猫抓病的病原体是猫阿菲波菌(Afipia felis),但现以确定汗氏巴尔通体(Bartonella henselae)是其病原.

  1. Detailed paragenesis and Li-mica compositions as recorders of the magmatic-hydrothermal evolution of the Maoping W-Sn deposit (Jiangxi, China)

    Science.gov (United States)

    Legros, Hélène; Marignac, Christian; Mercadier, Julien; Cuney, Michel; Richard, Antonin; Wang, Ru-Cheng; Charles, Nicolas; Lespinasse, Marc-Yves

    2016-11-01

    Li-micas have been used as indicators of the evolution of granites. However, hydrothermal Li-micas are less documented. World-class W-Sn deposits associated with Early Yanshanian granites (South Jiangxi, China) show magmatic and hydrothermal Li-micas which could help unravelling the magmatic-hydrothermal evolution of rare metal deposits. Six types of Li-micas have been identified in the vein system of the Maoping W-Sn deposit through detailed petrography and EPMA and LA-ICP-MS analyses, by chronological order: (i) late-magmatic Li-micas in feldspar veins, associated with late crystallization of a peraluminous melt; (ii) hydrothermal Fe-Li micas (Fe-Li mica veins and selvages); (iii) hydrothermal Fe-Li micas in W-Sn veins; (iv) Fe-Li micas in later banded quartz veins; (v) Li-muscovite in the final stages; and finally (vi) micas associated with alteration at each stage. Based on oscillatory variations and trends in major elements composition, the chemical variations in Li-micas from the successive stages and in hydrothermal micas that crystallized in the veins are interpreted to reflect mixing between at least three fluids of possible magmatic, meteoric and metamorphic origins. The crystallization of zircons and REE minerals, combined with variations of major and trace element concentrations in the Li-micas, notably an enrichment of rare metals (W-Sn-Ta-Nb) in the Li-micas, implies emplacement of a hidden peralkaline REE-rich magma during the crystallization of the banded quartz veins, a source which was different to the pre-existing peraluminous granites. The possible involvement of both peraluminous and peralkaline intrusives suggests the existence of polyphase magmatic-hydrothermal systems in the Maoping deposit, during the Yanshanian event (190-80 Ma).

  2. Presence of Bartonella species and Rickettsia species DNA in the blood, oral cavity, skin and claw beds of cats in the United States.

    Science.gov (United States)

    Lappin, Michael R; Hawley, Jennifer

    2009-10-01

    The purpose of this study was to determine the prevalence of Bartonella species and Rickettsia species DNA in the blood, oral cavity, skin and claw beds of feral cats without evidence of skin disease that were housed in Alabama (n = 24), Florida (n = 27) and Colorado (n = 32). Samples were assessed by use of polymerase chain reaction assays. The Bartonella species IgG prevalence was also determined. While Bartonella species DNA was not amplified from any sample from Colorado cats, it was commonly amplified from blood (56.9%), skin (31.4%), claws (17.6%) and gingiva (17.6%) of the 51 cats housed in Alabama and Florida. All 10 flea groups assessed in this study were infected with a Bartonella species or R. felis. Bartonella species IgG titres did not accurately predict bacteraemia (positive predictive value = 57.1%; negative predictive value = 82.1%). Bartonella species DNA was amplified from blood of cats with and without C. felis. Rickettsia felis DNA was only detected in or on the skin of one cat and the gingiva of an additional cat. It was concluded that cats can be an occupational health risk for veterinarians, particularly in areas with high prevalence of Ctenocephalides felis. Further study is required to determine whether Bartonella species or Rickettsia species infections of cats are associated with dermatological disease. The combination of Bartonella species serological test results with Bartonella species PCR or culture is likely to give the most accurate information concerning the current infection status of individual cats.

  3. Hydrazine Blending and Storage Facility, Interim Response Action Implementation. Final Safety Plan

    Science.gov (United States)

    1989-08-30

    PAvleved by wanagar H/S: 0n’e: CQ3oes to: Project onaagar, Offi c aInuagm., OSO. Corp•rate HealtI and S4fely Manager 318 10.0 EMERGENCY PROCEDURES 10.1...constitute a safety problem if activities such as ladder climbing are required. B-7 0 H M HEALTH AND SAFETY PROCEDURES NUMBER 14 PAGE SUBJECT: SELF

  4. The ancestral karyotype of Carnivora: comparison with that of platyrrhine monkeys.

    Science.gov (United States)

    Dutrillaux, B; Couturier, J

    1983-01-01

    The karyotypes of six species of Carnivora (Mungos mungo, Paradoxurus hermaphroditus, Potos flavus, Mustela furo, Felis serval, and Halichoerus grypus), representative of five different families, were studied and compared. Correspondence between almost all chromosome segments was found, and a presumed ancestral karyotype of Carnivora is proposed. Analogies to human chromosomes are also given, and the results obtained are in excellent agreement with previously published gene mapping data on man and the domestic cat.

  5. Case Series: Chikungunya and Dengue at a Forward Operating Location

    Science.gov (United States)

    2015-05-01

    ES) USAF School of Aerospace Medicine Public Health and Preventive Medicine Department/PHR 2510 Fifth St. Wright-Patterson AFB, OH 45433-7913 8...chikungunya.8 Th e mosquito vectors of dengue and chikungu- nya viruses in the Caribbean are the same species, Aedes albopictus and Aedes aegypti...transmission of Mayaro virus by Aedes aegypti. Am J Trop Med Hyg. 2011;85(4):750–757. 13. Wolf SP, Reeves WK. Rickettsia felis (Rickettsiales

  6. Tooele Army Depot Revised Final Site-Wide Ecological Risk Assessment. Volume I.

    Science.gov (United States)

    1998-02-01

    Cerridae Deer Cervus canadensis Elk 2 Odocoileus hemionus Mule deer X 1 Order: Carnivora Carnivores Family: Canidae Wolves, Foxes, and the Coyote...NA NA Y Cat Mammalia Carnivora Felidae Felis domesticus N Cow Mammalia Artiodactyla Bovidae Bos taurus N Dog Mammalia Carnivore Canidae Canis...fauna Invertebrata Varies Varies No NA Plants Plantae NA NA Yes (Ute ladies’ tresses, Clay phacelia) Yes ♦ This receptor is a Special Status

  7. Comparative Studies of Estrous Synchronization, Ovulation Induction, Luteal Function and Embryo Cryopreservation in Domestic Sheep and Application to Related Nondomestic Ungulate Species

    Science.gov (United States)

    1989-12-20

    viruses (infectious bovine rhinotracheitis virus, IBRV, Singh et al., 1982; vesicular stomatitis virus, VSV, Singh and Thomas, 1987) have proven...vesicular stomatitis virus, Singh and Thomas, 1987). Although porcine embryos are susceptible to infection under in vitro conditions, in the presence...Theriogenology 23: 190 (abstr.), 1985. Dresser, B.L., E.J. Gelwicks, K.B. Wachs and G.L. Keller. First successful transfer of cryopreserved feline (Felis

  8. Dicty_cDB: VSI381 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 006861 |pid:none) Chlamydophila felis Fe/C-56 DNA,... 35 3.5 D38156_1( D38156 |pid:none) Equine rotavirus RN...8155 |pid:none) Equine rotavirus RNA 5 for NSP1, compl... 35 3.5 AP006861_626( AP...ides DSM... 35 3.5 CP000409_286( CP000409 |pid:none) Rickettsia canadensis str. McKie... 35 3.5 D38155_1( D3

  9. Evolutionary analysis of a large mtDNA translocation (numt) into the nuclear genome of the Panthera genus species.

    Science.gov (United States)

    Kim, Jae-Heup; Antunes, Agostinho; Luo, Shu-Jin; Menninger, Joan; Nash, William G; O'Brien, Stephen J; Johnson, Warren E

    2006-02-01

    Translocation of cymtDNA into the nuclear genome, also referred to as numt, has been reported in many species, including several closely related to the domestic cat (Felis catus). We describe the recent transposition of 12,536 bp of the 17 kb mitochondrial genome into the nucleus of the common ancestor of the five Panthera genus species: tiger, P. tigris; snow leopard, P. uncia; jaguar, P. onca; leopard, P. pardus; and lion, P. leo. This nuclear integration, representing 74% of the mitochondrial genome, is one of the largest to be reported in eukaryotes. The Panthera genus numt differs from the numt previously described in the Felis genus in: (1) chromosomal location (F2-telomeric region vs. D2-centromeric region), (2) gene make up (from the ND5 to the ATP8 vs. from the CR to the COII), (3) size (12.5 vs. 7.9 kb), and (4) structure (single monomer vs. tandemly repeated in Felis). These distinctions indicate that the origin of this large numt fragment in the nuclear genome of the Panthera species is an independent insertion from that of the domestic cat lineage, which has been further supported by phylogenetic analyses. The tiger cymtDNA shared around 90% sequence identity with the homologous numt sequence, suggesting an origin for the Panthera numt at around 3.5 million years ago, prior to the radiation of the five extant Panthera species.

  10. Temperature dependence of small polaron population decays in iron-doped lithium niobate by Monte Carlo simulations

    Science.gov (United States)

    Mhaouech, I.; Guilbert, L.

    2016-10-01

    The population decay of light-induced small polarons in iron-doped lithium niobate is simulated by a Monte-Carlo method on the basis of Holstein's theory. The model considers random walks of both bound polarons (NbLi4+) and free polarons (NbNb4+) ending to deep traps (FeLi3+). The thermokinetic interplay between polaron species is introduced by trapping and de-trapping rates at niobium antisites (NbLi). The decay of the NbLi4+ population proceeds by three possible channels: direct trapping at FeLi3+ sites, hopping on niobium antisites and hopping on Nb regular sites after conversion to the free state. Up to three regimes, each one reflecting the predominance of one of these processes, appear with different activation energies in the Arrhenius plots of the decay time. The influence of FeLi and NbLi concentrations on the transition temperatures is evidenced. For both polaron species, the length of the final hop (trapping length) is found much larger than the usual hopping length and decreases at rising temperature. This trap size effect is a natural consequence of Holstein's theory and may explain some unclear features of polaron-related light-induced phenomena, such as the temperature-dependent stretching exponent of light-induced absorption decays and the anomalous increase of the photoconductivity at high doping levels.

  11. Infection Rates of Wolbachia sp. and Bartonella sp. in Different Populations of Fleas.

    Science.gov (United States)

    Zurita, Antonio; Gutiérrez, Sara García; Cutillas, Cristina

    2016-11-01

    In the present study, a molecular detection of Bartonella sp. and Wolbachia sp. in Ctenocephalides felis (Siphonaptera: Pulicidae) isolated from Canis lupus familiaris from different geographical areas of Spain, Iran and South Africa, and in Stenoponia tripectinata tripectinata isolated from Mus musculus from the Canary Islands has been carried out by amplification of the 16S ribosomal RNA partial gene of Wolbachia sp. and intergenic spacer region (its region) of Bartonella sp. A total of 70 % of C. felis analysed were infected by W. pipientis. This percentage of prevalence was considerably higher in female fleas than in male fleas. Bartonella DNA was not detected in C. felis from dogs, while Bartonella elizabethae was detected and identified in S. t. tripectinata from M. musculus from the Canary Islands representing 43.75 % prevalence. This report is the first to identify B. elizabethae in S. t. tripectinata collected in M. musculus from the Canary Islands. Thus, our results demonstrate that this flea is a potential vector of B. elizabethae and might play roles in human infection. The zoonotic character of this bartonellosis emphasizes the need to alert public health authorities and the veterinary community of the risk of infection.

  12. Prevalence of selected infectious disease agents in stray cats in Catalonia, Spain

    Directory of Open Access Journals (Sweden)

    Sara Ravicini

    2016-02-01

    Full Text Available Objectives The objective of the current study was to investigate the prevalence rates of the following infectious agents in 116 stray cats in the Barcelona area of Spain: Anaplasma phagocytophilum, Bartonella species, Borrelia burgdorferi, Chlamydia felis, Dirofilaria immitis, Ehrlichia species, feline calicivirus (FCV, feline herpesvirus-1 (FHV-1, feline leukaemia virus (FeLV, feline immunodeficiency virus (FIV, haemoplasmas, Mycoplasma species and Rickettsia species. Methods Serum antibodies were used to estimate the prevalence of exposure to A phagocytophilum, Bartonella species, B burgdorferi, Ehrlichia species and FIV; serum antigens were used to assess for infection by D immitis and FeLV; and molecular assays were used to amplify nucleic acids of Anaplasma species, Bartonella species, C felis, D immitis, Ehrlichia species, FCV, FHV-1, haemoplasmas, Mycoplasma species and Rickettsia species from blood and nasal or oral swabs. Results Of the 116 cats, 63 (54.3% had evidence of infection by Bartonella species, FeLV, FIV or a haemoplasma. Anaplasma species, Ehrlichia species or Rickettsia species DNA was not amplified from these cats. A total of 18/116 cats (15.5% were positive for FCV RNA (six cats, Mycoplasma species DNA (six cats, FHV-1 DNA (three cats or C felis DNA (three cats. Conclusions and relevance This study documents that shelter cats in Catalonia are exposed to many infectious agents with clinical and zoonotic significance, and that flea control is indicated for cats in the region.

  13. Isolation of Candidatus Rickettsia asemboensis from Ctenocephalides Fleas.

    Science.gov (United States)

    Luce-Fedrow, Alison; Maina, Alice N; Otiang, Elkanah; Ade, Fredrick; Omulo, Sylvia; Ogola, Eric; Ochieng, Linus; Njenga, M Kariuki; Richards, Allen L

    2015-04-01

    Candidatus Rickettsia asemboensis was identified molecularly in fleas collected in 2009 from Asembo, Kenya. Multilocus sequence typing using the 17-kD antigen gene, rrs, gltA, ompA, ompB, and sca4 demonstrated that Candidatus R. asemboensis is closely related to Rickettsia felis but distinct enough to be considered for separate species classification. Following this molecular characterization of Candidatus R. asemboensis, the in vitro cultivation of this bacterium was then performed. We used Ctenocephalides canis and Ctenocephalides felis fleas removed from dogs in Kenya to initiate the in vitro isolation of Candidatus R. asemboensis. Successful cultures were obtained using Drosophila melanogaster S2 and Aedes albopictus C6/36 cell lines. Cytological staining and quantitative real-time PCR (qPCR) assays were used to visualize/confirm the culture of the bacteria in both cell lines. Sequencing of fragments of the 17-kD antigen gene, gltA, and ompB genes confirmed the identity of our Candidatus R. asemboensis isolates. To date, we have passaged Candidatus R. asemboensis 12 times through S2 and C6/36 cells, and active and frozen cultures are currently being maintained. This is the first time that a R. felis-like organism has been grown and maintained in culture and is therefore the first time that one of them, Candidatus R. asemboensis, has been characterized beyond molecular typing.

  14. Rickettsia species in fleas collected from small mammals in Slovakia.

    Science.gov (United States)

    Špitalská, Eva; Boldiš, Vojtech; Mošanský, Ladislav; Sparagano, Olivier; Stanko, Michal

    2015-11-01

    Epidemiological and epizootiological studies of Rickettsia felis and other Rickettsia spp. are very important, because their natural cycle has not yet been established completely. In total, 315 fleas (Siphonaptera) of 11 species of Ceratophyllidae, Hystrichopsyllidae and Leptopsyllidae families were tested for the presence of Rickettsia species and Coxiella burnetii with conventional and specific quantitative real-time PCR assays. Fleas were collected from five rodent hosts (Myodes glareolus, Apodemus flavicollis, Apodemus agrarius, Microtus subterraneus, Microtus arvalis) and three shrew species (Sorex araneus, Neomys fodiens, Crocidura suaveolens) captured in Eastern and Southern Slovakia. Overall, Rickettsia spp. was found in 10.8% (34/315) of the tested fleas of Ctenophthalmus agyrtes, Ctenophthalmus solutus, Ctenophthalmus uncinatus and Nosopsyllus fasciatus species. Infected fleas were coming from A. flavicollis, A. agrarius, and M. glareolus captured in Eastern Slovakia. C. burnetii was not found in any fleas. R. felis, Rickettsia helvetica, unidentified Rickettsia, and rickettsial endosymbionts were identified in fleas infesting small mammals in the Košice region, Eastern Slovakia. This study is the first report of R. felis infection in C. solutus male flea collected from A. agrarius in Slovakia.

  15. Genotypic characterization and phylogenetic analysis of Cryptosporidium sp. from domestic animals in Brazil.

    Science.gov (United States)

    Huber, F; da Silva, S; Bomfim, T C B; Teixeira, K R S; Bello, A R

    2007-11-30

    The purpose of the present study was the genetic characterization, sequencing and phylogenetic analysis of 18S rDNA sequences of Cryptosporidium isolates obtained from different animal hosts in Brazil. Fecal samples containing Cryptosporidium oocysts were obtained from chickens, ducks, quails, guinea pigs, dairy calves, dogs and cats. For amplification of 18S rDNA sequences the Secondary-PCR product of the extracted DNA from fecal suspension of each studied animal was utilized. The primary genetic characterization of Cryptosporidium sp. was performed using RFLP with the enzymes SspI and VspI. DNA samples were sequenced and subjected to phylogenetic analysis. The results showed C. baileyi infecting two ducks and one quail and C. melagridis infecting one chicken. The sequences obtained from Cryptosporidium sp. infecting guinea pigs were not identified within groups of known Cryptosporidium species. The isolates found parasitizing cats and one dog were diagnosed as C. felis and C. canis, respectively. One isolate of calf origin was identified as C. parvum. The phylogenetic analysis showed clear distribution of isolates between two Cryptosporidium sp. groups according to their gastric or intestinal parasitism. A great genetic distance was observed between C. felis and C. canis from Brazil when compared to the reference sequences obtained from GenBank. The results obtained during this study constitute the first report of rDNA sequences from C. baileyi, C. meleagridis, C. felis, C. canis and C. parvum isolated in Brazil.

  16. IMMUNOHISTOCHEMISTRY VERSUS IMMUNOFLUORESENCE IN THE DIAGNOSIS OF AUTOIMMUNE BLISTERING DISEASES

    Directory of Open Access Journals (Sweden)

    Ana Maria Abreu Velez

    2013-11-01

    Full Text Available Introduction: In patients with autoimmune skin blistering diseases (ABDs, the diagnostic gold standard has classically been direct and indirect immunofluorescence (DIF and IIF, despite inherent technical problems of autofluorescence. Aim: We sought to overcome autofluorescence issues and compare the reliability of immunofluorescence versus immunohistochemistry (IHC staining in the diagnoses of these diseases. Methods: We tested via IHC for anti-human IgG, IgM, IgA, IgD, IgE, Kappa light chains, Lambda light chains, Complement/C3c, Complement/C1q, Complement/C3d, albumin and fibrinogen in 30 patients affected by a new variant of endemic pemphigus foliaceus in El Bagre, Colombia (El Bagre-EPF, and 30 control biopsies from the endemic area. We also tested archival biopsies from patients with ABDs whose diagnoses were made clinically, histopathologically and by DIF/IIF studies from 2 independent dermatopathology laboratories in the USA. Specifically, we tested 34 patients with bullous pemphigoid (BP, 18 with pemphigus vulgaris (PV, 8 with pemphigus foliaceus (PF, 14 with dermatitis herpetiformis (DH and 30 control skin samples from plastic esthetic surgery reduction surgeries. Results: The diagnostic correlation between IHC and DIF-IIF was almost 98% in most cases. IHC revealed evidence of autofluorescence around dermal blood vessels, dermal eccrine glands and neurovascular packages feeding skin appendices in ABDs; this autofluorescence may represent a non-specific immune response. Strong patterns of positivity were seen also in endothelial-mesenchymal cell junction-like structures, as well as between dermal fibrohistiocytic cells. In PV, we noted strong reactivity to neurovascular packages supplying sebaceous glands, as well as apocrine glands with edematous changes. Conclusions: We suggest that IHC is as reliable as DIF or IIF for the diagnosis of ABDs; our findings further suggest that what has previously been considered DIF/IIF autofluorescence

  17. Malaria in gold-mining areas in Colombia.

    Science.gov (United States)

    Castellanos, Angélica; Chaparro-Narváez, Pablo; Morales-Plaza, Cristhian David; Alzate, Alberto; Padilla, Julio; Arévalo, Myriam; Herrera, Sócrates

    2016-01-01

    Gold-mining may play an important role in the maintenance of malaria worldwide. Gold-mining, mostly illegal, has significantly expanded in Colombia during the last decade in areas with limited health care and disease prevention. We report a descriptive study that was carried out to determine the malaria prevalence in gold-mining areas of Colombia, using data from the public health surveillance system (National Health Institute) during the period 2010-2013. Gold-mining was more prevalent in the departments of Antioquia, Córdoba, Bolívar, Chocó, Nariño, Cauca, and Valle, which contributed 89.3% (270,753 cases) of the national malaria incidence from 2010-2013 and 31.6% of malaria cases were from mining areas. Mining regions, such as El Bagre, Zaragoza, and Segovia, in Antioquia, Puerto Libertador and Montelíbano, in Córdoba, and Buenaventura, in Valle del Cauca, were the most endemic areas. The annual parasite index (API) correlated with gold production (R2 0.82, p Colombia.

  18. Crecimiento de alevines de Ictalurus Balsanus (pisces:ictaluridae en condiciones de laboratorio.

    Directory of Open Access Journals (Sweden)

    Jorge Luna-Figueroa

    2016-03-01

    Full Text Available Se realizó un estudio para evaluar el efecto de tres dietas sobre el crecimiento de alevines de Ictalurus balsanus en condiciones de laboratorio. La talla inicial de los peces fue 39.68 ± 2.0 mm de longitud total y 411.47 ± 17.00 mg de peso corporal, colocados en grupos de 10 peces por acuario de 80L. Los bagres fueron alimentados con tres dietas diferentes: I(31,13% proteína, 2,70% grasa, 56,53% carbohidratos, II(39,12%, 3,0%, 35,0%, III(53,57%, 19,37%, 4,17%. Los resultados indican que el incremento en el peso y en la longitud total de I. balsanus fueron mayores (P0.05 entre los peces nutridos con los distintos alimentos. Por lo que se concluye que las características propias del alimento vivo y, en particular, la alta concentración proteica de Daphnia sp. Generaron mayor incremento en la tasa de crecimiento especifico de I. balsanus.

  19. Estudio preliminar de ictioplancton de la Amazonia peruana con énfasis en la familia Pimelodidae

    Directory of Open Access Journals (Sweden)

    María Rojas

    2013-05-01

    Full Text Available En el presente trabajo se informa y describen las larvas de peces presentes en la naciente del río Madre de Dios, con énfasis en la familia Pimelodidae. Las colectas se realizaron entre noviembre y diciembre de 2004, utilizando una red de ictioplancton (300 micras de malla, en 8 transectos que cubrieron un tramo aproximado de 2 km aguas arriba y aguas abajo de la ciudad de Puerto Maldonado. Un total de 83750 larvas fueron examinadas, la mayor abundancia la presentaron los Characiformes (81%, seguido por Siluriformes (18%. En la familia Pimelodidae, se identificaron 12 especies de consumo, destacando Pseudoplatystoma tigrinum, P. fasciatum y Sorubim lima. Los principales órdenes encontrados en el ictioplancton siguen un patrón de abundancia relativa similar al estado adulto. La presencia de larvas de los grandes bagres migradores (familia Pimelodidae en la zona de muestreo sugiere que esta parte del río Madre de Dios es un área de reproducción, y por ende de importancia para la conservación de estas especies.

  20. Malaria in gold-mining areas in Colombia

    Directory of Open Access Journals (Sweden)

    Angélica Castellanos

    2016-01-01

    Full Text Available Gold-mining may play an important role in the maintenance of malaria worldwide. Gold-mining, mostly illegal, has significantly expanded in Colombia during the last decade in areas with limited health care and disease prevention. We report a descriptive study that was carried out to determine the malaria prevalence in gold-mining areas of Colombia, using data from the public health surveillance system (National Health Institute during the period 2010-2013. Gold-mining was more prevalent in the departments of Antioquia, Córdoba, Bolívar, Chocó, Nariño, Cauca, and Valle, which contributed 89.3% (270,753 cases of the national malaria incidence from 2010-2013 and 31.6% of malaria cases were from mining areas. Mining regions, such as El Bagre, Zaragoza, and Segovia, in Antioquia, Puerto Libertador and Montelíbano, in Córdoba, and Buenaventura, in Valle del Cauca, were the most endemic areas. The annual parasite index (API correlated with gold production (R2 0.82, p < 0.0001; for every 100 kg of gold produced, the API increased by 0.54 cases per 1,000 inhabitants. Lack of malaria control activities, together with high migration and proliferation of mosquito breeding sites, contribute to malaria in gold-mining regions. Specific control activities must be introduced to control this significant source of malaria in Colombia.

  1. [Impact of water resource installations on the distribution of schistosomiasis and its intermediary hosts in Burkina Faso].

    Science.gov (United States)

    Poda, Jean-Noël; Sondo, Blaise; Parent, Gérard

    2003-01-01

    Dams generally are a favourable biotope for the molluscs acting as intermediary hosts to schistosomiasis. The importance of the schistosomiasis endemic which follows depends on the interactions taking place between the parasites and their definitive (humans) and intermediary hosts. A preliminary sound knowledge of the prevailing epidemiological situations is therefore necessary to define an efficient programme to fight these infections. The extension of schistosomiasis following the installation of water resource facilities is significative of the part played by these hosts. In the hydroagricultural complex of Sourou, the prevalence of urinary schistosomiasis increased from 19% in 1954 to more than 70% in 1998-1999 in Guiédougou, the most ancient site. As to digestive schistosomiasis, almost unheard of until 1987, its prevalence ranged from 8% to 69% in 1998 in the villages located alongside the areas thus equipped. In the Kou Valley, the prevalence went up from 14% in 1957 to 80% in 1974 for urinary schistosomiasis and from 1.3% to 45% for intestinal schistosomiasis. The same tendencies are likely to appear in the hydraulic installations of Bagré, Ziga, and Kompienga. Dams thus constitute amplifying factors for the proliferation of species and for parasite-host interactions. All the actors (developers, populations and scientists) are faced with the challenge of finding a mean to control the development of schistosomiasis infections which are likely to seriously lessen the benefits expected from these hydraulic installations.

  2. Preliminary results of mercury levels in raw and cooked seafood and their public health impact.

    Science.gov (United States)

    Costa, Fernanda do N; Korn, Maria Graças A; Brito, Geysa B; Ferlin, Stacy; Fostier, Anne H

    2016-02-01

    Mercury is toxic for human health and one of the main routes of exposure is through consumption of contaminated fish and shellfish. The objective of this work was to assess the possible mercury contamination of bivalves (Anomalocardia brasiliana, Lucina pectinata, Callinectes sapidus), crustacean (C. sapidus) and fish (Bagre marinus and Diapterus rhombeus) collected on Salinas da Margarida, BA (Brazil), a region which carciniculture, fishing and shellfish extraction are the most important economic activities. The effect of cooking on Hg concentration in the samples was also studied. The results showed that Hg concentration was generally higher in the cooked samples than in raw samples. This increase can be related to the effect of Hg pre-concentration, formation of complexes involving mercury species and sulfhydryl groups present in tissues and/or loss of water and fat. The highest concentrations were found in B. marinus samples ranging 837.0-1585.3 μg kg(-1), which exceeded those recommended by Brazilian Health Surveillance Agency (ANVISA). In addition, Hg values found in the other samples also suggest the monitoring of the Hg concentrations in seafood consumed from the region.

  3. Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips

    Directory of Open Access Journals (Sweden)

    Silvia Tamie Matsumoto

    2006-01-01

    Full Text Available Cytotoxicity of metals is important because some metals are potential mutagens able to induce tumors in humans and experimental animals. Chromium can damage DNA in several ways, including DNA double strand breaks (DSBs which generate chromosomal aberrations, micronucleus formation, sister chromatid exchange, formation of DNA adducts and alterations in DNA replication and transcription. In our study, water samples from three sites in the Córrego dos Bagres stream in the Franca municipality of the Brazilian state of São Paulo were subjected to the comet assay and micronucleus test using erythrocytes from the fish Oreochromis niloticus. Nuclear abnormalities of the erythrocytes included blebbed, notched and lobed nuclei, probably due to genotoxic chromium compounds. The greatest comet assay damage occurred with water from a chromium-containing tannery effluent discharge site, supporting the hypothesis that chromium residues can be genotoxic. The mutagenicity of the water samples was assessed using the onion root-tip cell assay, the most frequent chromosomal abnormalities observed being: c-metaphases, stick chromosome, chromosome breaks and losses, bridged anaphases, multipolar anaphases, and micronucleated and binucleated cells. Onion root-tip cell mutagenicity was highest for water samples containing the highest levels of chromium.

  4. TISSUE INHIBITOR OF METALLOPROTEINASE 1, MATRIX METALLOPROTEINASE 9, ALPHA-1 ANTITRYPSIN, METALLOTHIONEIN AND UROKINASE TYPE PLASMINOGEN ACTIVATOR RECEPTOR IN SKIN BIOPSIES FROM PATIENTS AFFECTED BY AUTOIMMUNE BLISTERING DISEASES

    Directory of Open Access Journals (Sweden)

    Ana Maria Abreu Velez

    2013-07-01

    Full Text Available Introduction: Proteinases and proteinase inhibitors have been described to play a role in autoimmune skin blistering diseases. We studied skin lesional biopsies from patients affected by several autoimmune skin blistering diseases for proteinases and proteinase inhibitors. Methods: We utilized immunohistochemistry to evaluate biopsies for alpha-1-antitrypsin, human matrix metalloproteinase 9 (MMP9, human tissue inhibitor of metalloproteinases 1 (TIMP-1, metallothionein and urokinase type plasminogen activator receptor (uPAR. We tested 30 patients affected by endemic pemphigus, 30 controls from the endemic area, and 15 normal controls. We also tested 30 biopsies from patients with bullous pemphigoid (BP, 20 with pemphigus vulgaris (PV, 8 with pemphigus foliaceus, and 14 with dermatitis herpetiformis (DH. Results: Contrary to findings in the current literature, most autoimmune skin blistering disease biopsies were negative for uPAR and MMP9. Only some chronic patients with El Bagre-EPF were positive to MMP9 in the dermis, in proximity to telocytes. TIMP-1 and metallothionein were positive in half of the biopsies from BP patients at the basement membrane of the skin, within several skin appendices, in areas of dermal blood vessel inflammation and within dermal mesenchymal-epithelial cell junctions.

  5. Bartonella species in fleas from Palestinian territories: prevalence and genetic diversity.

    Science.gov (United States)

    Nasereddin, A; Risheq, A; Harrus, S; Azmi, K; Ereqat, S; Baneth, G; Salant, H; Mumcuoglu, K Y; Abdeen, Z

    2014-12-01

    Bartonellosis is an infectious bacterial disease. The prevalence and genetic characteristics of Bartonella spp. in fleas of wild and domestic animals from Palestinian territories are described. Flea samples (n=289) were collected from 121 cats, 135 dogs, 26 hyraxes and seven rats from northern (n=165), central (n=113), and southern Palestinian territories (n=11). The prevalent flea species were: Ctenocephalides felis (n=119/289; 41.2%), Ctenocephalides canis (n=159/289; 55%), and Xenopsylla sp. (n=7/289; 2.4%). Targeting the Intergenic Transcribed Spacer (ITS) locus, DNA of Bartonella was detected in 22% (64/289) of all fleas. Fifty percent of the C. felis and 57% of the Xenopsylla sp. contained Bartonella DNA. DNA sequencing showed the presence of Bartonella clarridgeiae (50%), Bartonella henselae (27%), and Bartonella koehlerae (3%) in C. felis. Xenopsylla sp. collected from Rattus rattus rats were infected with Bartonella tribocorum, Bartonella elizabethae, and Bartonella rochalimae. Phylogenetic sequence analysis using the 16S ribosomal RNA gene obtained four genetic clusters, B. henselae and B. koehlerae as subcluster 1, B. clarridgeiae as cluster 2, while the rat Bartonella species (B. tribocorum and B. elizabethae) were an outgroup cluster. These findings showed the important role of cat and rat fleas as vectors of zoonotic Bartonella species in Palestinian territories. It is hoped that this publication will raise awareness among physicians, veterinarians, and other health workers of the high prevalence of Bartonella spp. in fleas in Palestinian territories and the potential risk of these pathogens to humans and animals in this region.

  6. Molecular characterization of Cryptosporidium spp. from HIV infected patients from an urban area of Brazil Caracterização molecular de Cryptosporidium spp. de pacientes de área urbana do Brasil infectados por HIV

    Directory of Open Access Journals (Sweden)

    Patrícia de Lucca

    2009-12-01

    Full Text Available Cryptosporidium spp. are important cause of enteric disease in humans, but may also infect animals. This study describes the relative frequency of several Cryptosporidium species found in human specimens from HIV infected patients in the São Paulo municipality obtained from January to July 2007. Sequence analysis of the products of nested-PCR based on small subunit rRNA and Cryptosporidium oocyst wall protein coding genes revealed 17 (63.0% isolates of C. hominis, four (14.8% C. parvum, five (18.5% C. felis and one (3.7% C. canis. These findings suggest that, in urban environments of Brazil, the cat adapted C. felis may play a potential role in the zoonotic transmission of cryptosporidiosis whereas the anthroponotic transmission of cryptosporidiosis caused by C. hominis seems to predominate.Cryptosporidium spp. são importantes causas de doenças entéricas em humanos, mas podem também ser encontrados em animais. O presente estudo descreve a frequência relativa de diversas espécies de Cryptosporidium em amostras de humanos da cidade de São Paulo, Brasil, obtidas de janeiro a julho de 2007. Análises de sequências de produtos de nested PCR direcionadas ao genes codificadores da menor unidade ribosomal e da proteina de parede de oocistos revelaram 17 (63,0% isolados de C. hominis, quatro (14,8% C. parvum, cinco (18,5% C. felis, e um (3,7% C. canis. Estes resultados sugerem que, em ambientes urbanos no Brasil, o genótipo adaptado ao gato pode desempenhar potencial papel na transmissão zoonótica de criptosporidiose, enquanto a transmissão antroponótica da criptosporidiose causada pelo C. hominis parece predominar.

  7. Dicty_cDB: Contig-U10274-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available :none) Burkholderia vietnamiensis G4 c... 75 2e-12 CR378672_161( CR378672 |pid...:none) Methanosarcina mazei strain Goe1... 89 1e-16 CP000053_198( CP000053 |pid:none) Rickettsia felis URRWXCal2, co...romyxobacter sp. K, complete... 87 7e-16 AL939131_102( AL939131 |pid:none) Streptomyces coelicolor A3(2) co... 3500... 79 1e-13 AL939112_114( AL939112 |pid:none) Streptomyces coelicolor A3(2) co....la str. Sg (Schizaphis graminum)... 44 7.1 1 >( C94358 ) Dictyostelium discoideum slug

  8. NFC(Near Field Communication)を用いたAndroidスマートフォンによる授業出席管理システムの開発

    OpenAIRE

    市村,匠; 鎌田,真

    2012-01-01

    Recently, the IC chip embedded cards have been widely used in our daily life. Especially, FeliCa is well known card system, which is a contact-less RFID smart card system from Sony in Japan. Felica is used in electronic money cards such as Rakuten-Edy. The attendance management is a troublesome job in a lecture, because the check of the number attendance requires the authenti cation of stundets. We developed the attendance management system with Android Smartphone, where the authentication...

  9. Approach for classification and taxonomy within family Rickettsiaceae based on the Formal Order Analysis.

    Science.gov (United States)

    Shpynov, S; Pozdnichenko, N; Gumenuk, A

    2015-01-01

    Genome sequences of 36 Rickettsia and Orientia were analyzed using Formal Order Analysis (FOA). This approach takes into account arrangement of nucleotides in each sequence. A numerical characteristic, the average distance (remoteness) - "g" was used to compare of genomes. Our results corroborated previous separation of three groups within the genus Rickettsia, including typhus group, classic spotted fever group, and the ancestral group and Orientia as a separate genus. Rickettsia felis URRWXCal2 and R. akari Hartford were not in the same group based on FOA, therefore designation of a so-called transitional Rickettsia group could not be confirmed with this approach.

  10. Lacrimal gland involvement in Kikuchi-Fujimoto disease.

    Science.gov (United States)

    Chavis, Pamela S.; Fallata, Amna; Al-Hussein, Hailah; Clunie, David; Huaman, Antonio

    1998-06-01

    PURPOSE. A 32-year-old Saudi female presented with typical Kikuchi-Fujimoto disease, i.e., fever, cervical lymphadenitis and leukopenia, but there was also painful upper eyelid swelling with pain on upgaze. METHODS. A connective tissue disease and lymphoma workup were unremarkable, as were antibody titers to Apifia felis and Bartonella henselae. RESULTS. Orbital computed tomography showed significant lacrimal gland enlargement. Cervical node biopsy revealed necrotizing lymphadenitis. CONCLUSION. Concomitant lacrimal gland inflammation and cervical lymphadenopathy may be a benign self-limited disease.

  11. The dog louse Heterodoxus spiniger from stray cats in Penang, Malaysia.

    Science.gov (United States)

    Norhidayu, S; Mohd Zain, S N; Jeffery, J; Lewis, J W

    2012-06-01

    Stray cats collected from Georgetown, Penang from 2008 to 2010 were screened for ectoparasites via fine-tooth combing. Two cats from a total 102 examined were infested with the dog louse, Heterodoxus spiniger. Both cats, a juvenile male and female were found in close contact with each other prior to capture. The number of lice ranged from 5 and 14 in the male and female cat respectively. Other ectoparasites recovered included the common cat flea, Ctenocephalides felis, one louse species Felicola subrostratus, one tick species Haemaphysalis bispinosa and one mite species of Listrophoridae. The present study reports for the first time the finding of H. spiniger on cats from peninsular Malaysia.

  12. Serosurvey of Rickettsia spp. in dogs and humans from an endemic area for Brazilian spotted fever in the State of São Paulo, Brazil Sorologia para Rickettsia spp. em cães e humanos de uma área endêmica para febre maculosa brasileira no Estado de São Paulo, Brasil

    Directory of Open Access Journals (Sweden)

    Adriano Pinter

    2008-02-01

    Full Text Available The present study provides a rickettsial serosurvey in 25 dogs and 35 humans in an endemic area for Brazilian spotted fever in the State of São Paulo, where the tick Amblyomma aureolatum is the main vector. Testing canine and human sera by indirect immunofluorescence against four Rickettsia antigens (R. rickettsii, R. parkeri, R. felis and R. bellii showed that 16 (64% of canine sera and 1 (2.8% of human sera reacted to at least one of these rickettsial antigens with titers ³ 64. Seven canine sera and the single reactive human serum showed titers to R. rickettsii at least four times those of any of the other three antigens. The antibody titers in these 7 animals and 1 human were attributed to stimulation by R. rickettsii infection. No positive canine or human serum was attributed to stimulation by R. parkeri, R. felis, or R. bellii. Our serological results showed that dogs are important sentinels for the presence of R. rickettsii in areas where the tick A. aureolatum is the main vector of Brazilian spotted fever.Este estudo avaliou a ocorrência de anticorpos anti-Rickettsia em 25 cães e 35 humanos, em uma área endêmica para a febre maculosa brasileira no Estado de São Paulo, onde o principal vetor é o carrapato Amblyomma aureolatum. Soros dos cães e humanos foram testados pela técnica de imunofluorescência indireta contra quatro antígenos de riquétsias (R. rickettsii, R. parkeri, R. felis, R. bellii, mostrando que soros de 16 (64% cães e 1 (2,8% humano reagiram com títulos ³ 64 para pelo menos um dos antígenos de riquétsias. Sete soros caninos e o único soro humano reativo demonstraram títulos para R. rickettsii no mínimo quatro vezes maior do que aqueles para os outros antígenos de riquétsias. Os títulos de anticorpos nesses cães e um humano foram considerados homólogos a R. rickettsii, enquanto que nenhum soro de cão ou humano foi considerado reativamente homólogo para R. parkeri, R. felis ou R. bellii. Os

  13. 从12S rRNA和Cyt b基因部分序列研究13种猫科动物的分子系统关系%Phylogeny of 13 Felidae species from China based on partial 12S rRNA and Cyt b genes sequences

    Institute of Scientific and Technical Information of China (English)

    郑涛; 费荣梅; 吴孝兵

    2005-01-01

    为探讨中国猫科动物(Felidae)的系统发生关系,本文对中国产13种猫科动物的12S rRNA基因(约371 bp)和细胞色素b基因(Cyt b) 部分序列(约355 bp)进行了分析,并采用"最大简约法"和"最大似然法"构建了分子系统树.结果表明:在Cyt b基因序列中,有113个位点存在变异(约为总位点数的31.8%),高于12S rRNA基因序列的44个变异位点(约为总位点数的11.9%);构建的分子系统树显示,猞猁(Lynx lynx)可能是中国最早起源的猫科动物,与其它猫科动物之间的亲缘关系较远,支持将其立为猞猁属(Lynx)的观点;草原斑猫(Felis libyca)、丛林猫(Felis chaus)、兔狲(Otocolobus manul)和荒漠猫(Felis bieti)具有较近的亲缘关系,支持将兔狲划归于猫属(Felis)的观点;金猫(Caopuma temminckii)、云猫(Pardofelis marmorata)具有较近的亲缘关系,但它们与猫属物种之间的亲缘关系可能较远,不支持将它们划归于猫属;豹猫(Ponailurus ribengalensis)、渔猫(Prionailurus viverrinus)具有较近的亲缘关系,支持将它们同归于豹猫属(Ponailurus);云豹(Neofelis nebulosa)、豹(Panthera pardus)、雪豹(Uncia uncia)、虎(Panthera tigris)具有较近的亲缘关系,支持将它们同归于豹属(Panthera)的观点

  14. Efficacy of an imidacloprid/flumethrin collar against fleas and ticks on cats

    Directory of Open Access Journals (Sweden)

    Stanneck Dorothee

    2012-04-01

    Full Text Available Abstract Background The objectives of the studies listed here were to ascertain the therapeutic and sustained efficacy of 10% imidacloprid (w/w and 4.5% flumethrin (w/w incorporated in a slow-release matrix collar, against laboratory-infestations of fleas and ticks on cats. Efficacy was evaluated against the flea Ctenocephalides felis felis, and the ticks Ixodes ricinus, Amblyomma americanum and Rhipicephalus turanicus. The number of studies was so large that only a general overview can be presented in this abstract. Methods Preventive efficacy was evaluated by infesting groups of cats (n = 8-10 with C. felis felis and/or I. ricinus, A. americanum or R. turanicus at monthly intervals at least, for a period of up to 8 months. Efficacy against fleas was evaluated 24 to 48 h after treatment and 24 h after infestation, and against ticks at 6 h (repellent or 48 h (acaricidal after infestation. Efficacy against flea larvae was evaluated over a period of 8 months by incubating viable flea eggs on blanket samples after cat contact. In all cases efficacy was calculated by comparison with untreated negative control groups. Results Efficacy against fleas (24 h generally exceeded 95% until study termination. In vitro efficacy against flea larvae exceeded 92% until Day 90 and then declined to 67% at the conclusion of the study on Day 230. Sustained acaricidal (48 h efficacy over a period of eight months was consistently 100% against I. ricinus from Day 2 after treatment, 100% against A. americanum, except for 98.5% and 97.7% at two time-points, and between 94% and 100% against R. turanicus. From Day 2 until 8 months after treatment the repellent (6 h, efficacy was consistently 100% against I. ricinus, and between 54.8% and 85.4% against R. turanicus. Conclusion The rapid insecticidal and acaricidal properties of the medicated collars against newly- acquired infestations of fleas and ticks and their sustained high levels of preventive efficacy have been

  15. Toxocarosis neurológica: descripción de un caso clínico

    OpenAIRE

    Radman, Nilda Ester; Guardis, Mónica del Valle; Schamun, Alejandro; Testi, Adriana; Archelli, Susana Mónica; Fonrouge, Reinaldo Domingo; Santillán, Graciela

    2000-01-01

    La toxocarosis es una parasitosis producida por los nematodos Toxocara canis y Toxocara felis cuyos huéspedes naturales son los cánidos y félidos domésticos, respectivamente. La infección humana ocurre de manera accidental por la ingesta de huevos larvados presentes en la tierra, arena, agua o alimentos contaminados. Abarca una diversidad de presentaciones clínicas que sumadas a la inespecificidad de la sintomatología y a la dificultad para acceder a un diagnóstico de laboratorio preciso, lle...

  16. Dicty_cDB: VSD185 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available and Malpighian tubule cDNA library Ctenocephalides felis cDNA clone 3254-25, mRNA sequence. 48 2e-06 2 BQ476622 |BQ476622.1 curculio...3e06.g Curculio glandium cDNA Curculio glandium cDNA clone curculio...3e06 3', mRNA sequence. 46 0.46 1 BQ476340 |BQ476340.1 curculio3e06.b Curculio glandium cDNA Curc...ulio glandium cDNA clone curculio3e06 5', mRNA sequence. 46 0.46 1 AX345182 |AX345182.1 Sequence 253 from Pa

  17. Afoxolaner against fleas: immediate efficacy and resultant mortality after short exposure on dogs

    OpenAIRE

    Beugnet Frédéric; deVos Christa; Liebenberg Julian; Halos Lénaïg; Fourie Josephus

    2014-01-01

    The speed of efficacy of afoxolaner (NexGard®) against Ctenocephalides felis fleas was evaluated in two studies. Study A assessed the efficacy against existing fleas whereas study B assessed the efficacy against new infesting fleas. In study A, 12 dogs were allocated to the untreated group and 20 dogs to the treated group. All dogs were infested by 100 fleas each at Day −1, treated at Day 0 and flea combed at 2 h or at 6 h post treatment. In study B, 6 dogs were allocated to the untreated gro...

  18. Discovery of sarolaner: A novel, orally administered, broad-spectrum, isoxazoline ectoparasiticide for dogs.

    Science.gov (United States)

    McTier, Tom L; Chubb, Nathan; Curtis, Michael P; Hedges, Laura; Inskeep, Gregory A; Knauer, Christopher S; Menon, Sanjay; Mills, Brian; Pullins, Aleah; Zinser, Erich; Woods, Debra J; Meeus, Patrick

    2016-05-30

    The novel isoxazoline ectoparasiticide, sarolaner, was identified during a lead optimization program for an orally-active compound with efficacy against fleas and ticks on dogs. The aim of the discovery program was to identify a novel isoxazoline specifically for use in companion animals, beginning with de novo synthesis in the Zoetis research laboratories. The sarolaner molecule has unique structural features important for its potency and pharmacokinetic (PK) properties, including spiroazetidine and sulfone moieties. The flea and tick activity resides in the chirally pure S-enantiomer, which was purified to alleviate potential off-target effects from the inactive enantiomer. The mechanism of action was established in electrophysiology assays using CHO-K1 cell lines stably expressing cat flea (Ctenocephalides felis) RDL (resistance-to-dieldrin) genes for assessment of GABA-gated chloride channel (GABACls) pharmacology. As expected, sarolaner inhibited GABA-elicited currents at both susceptible (CfRDL-A285) and resistant (CfRDL-S285) flea GABACls with similar potency. Initial whole organism screening was conducted in vitro using a blood feeding assay against C. felis. Compounds which demonstrated robust activity in the flea feed assay were subsequently tested in an in vitro ingestion assay against the soft tick, Ornithodoros turicata. Efficacious compounds which were confirmed safe in rodents at doses up to 30mg/kg were progressed to safety, PK and efficacy studies in dogs. In vitro sarolaner demonstrated an LC80 of 0.3μg/mL against C. felis and an LC100 of 0.003μg/mL against O. turicata. In a head-to-head comparative in vitro assay with both afoxolaner and fluralaner, sarolaner demonstrated superior flea and tick potency. In exploratory safety studies in dogs, sarolaner demonstrated safety in dogs≥8 weeks of age upon repeated monthly dosing at up to 20mg/kg. Sarolaner was rapidly and well absorbed following oral dosing. Time to maximum plasma concentration

  19. DEVELOPMENT OF IT TRIAGE SYSTEM (TRACY) TO SHARE REGIONAL DISASTER MEDICAL INFORMATION

    Science.gov (United States)

    Numada, Muneyoshi; Hada, Yasunori; Ohara, Miho; Meguro, Kimiro

    We developed an IT triage system for collecting disaster medical information in real time. FeliCa cards and card readers are used to obtain the number and cond ition of patients. The system is composed of two elements. First, the number of patie nts for each triage level and the accepted number of patients in each diagnosis and treatment department are obtained in real time, including response for changing triage level. Second, this information can be shared among hospitals, the administration, and residents in real time who are searching for their family. A disaster drill utiliz ing this system was held at the University of Yamanashi Hospital with 450 participants.

  20. Bioepidemiologia de possíveis vetores e riquétsias na microrregião de Juiz de Fora, médio Paraibuna, Minas Gerais, Brasil

    OpenAIRE

    Nunes, Emília de Carvalho

    2014-01-01

    As riquetsioses, causadas por organismos do gênero Rickettsia, são zoonoses presentes em todos os continentes. Com exceção de Rickettsia felis e Rickettsia akari associadas, respectivamente, a pulgas e ácaros, riquétsias tem como vetores e/ou reservatórios carrapatos. No Brasil Amblyomma cajennense, Amblyomma aureolatum e Amblyomma ovale são os mais importantes vetores para humanos. O presente estudo teve por objetivo avaliar a circulação de Rickettsia sp. em diferentes regiões da cidade de J...

  1. Freqüência de parasitas intestinais em felinos mantidos em zoológicos Frequency of intestinal parasites in felines kept in zoos

    Directory of Open Access Journals (Sweden)

    G.C.K. Müller

    2005-08-01

    Full Text Available The endoparasites occurrence in felines confined in two Zoos, between December 1999 and April 2000, was studied. Fecal samples of 18 felines (Panthera tigris, Panthera leo, Felis serval, Panthera onca, Puma concolor, Leopardus tigrinus and Leopardus wiedii were collected and the methods of Faust, modified Baermann and Hoffmann, were used for fecal analyses. Three genera were identified in the feces: Trichuris spp., Toxocara spp. and Giardia spp. In the zoo of Pomerode, six animals (46% were infected by Trichuris spp. and/or Giardia spp. and all samples from the zoo of Brusque were infected by Trichuris spp., Toxocara spp. and Giardia spp.

  2. Chlamydial plasmids and bacteriophages.

    Science.gov (United States)

    Pawlikowska-Warych, Małgorzata; Śliwa-Dominiak, Joanna; Deptuła, Wiesław

    2015-01-01

    Chlamydia are absolute pathogens of humans and animals; despite being rather well recognised, they are still open for discovery. One such discovery is the occurrence of extrachromosomal carriers of genetic information. In prokaryotes, such carriers include plasmids and bacteriophages, which are present only among some Chlamydia species. Plasmids were found exclusively in Chlamydia (C.) trachomatis, C. psittaci, C. pneumoniae, C. suis, C. felis, C. muridarum and C. caviae. In prokaryotic organisms, plasmids usually code for genes that facilitate survival of the bacteria in the environment (although they are not essential). In chlamydia, their role has not been definitely recognised, apart from the fact that they participate in the synthesis of glycogen and encode proteins responsible for their virulence. Furthermore, in C. suis it was evidenced that the plasmid is integrated in a genomic island and contains the tetracycline-resistance gene. Bacteriophages specific for chlamydia (chlamydiaphages) were detected only in six species: C. psittaci, C. abortus, C. felis, C. caviae C. pecorum and C. pneumoniae. These chlamydiaphages cause inhibition of the developmental cycle, and delay transformation of reticulate bodies (RBs) into elementary bodies (EBs), thus reducing the possibility of infecting other cells in time. Plasmids and bacteriophages can be used in the diagnostics of chlamydioses; although especially in the case of plasmids, they are already used for detection of chlamydial infections. In addition, bacteriophages could be used as therapeutic agents to replace antibiotics, potentially addressing the problem of increasing antibiotic-resistance among chlamydia.

  3. Molecular Evidence of Different Rickettsia Species in Villeta, Colombia.

    Science.gov (United States)

    Faccini-Martínez, Álvaro A; Ramírez-Hernández, Alejandro; Forero-Becerra, Elkin; Cortés-Vecino, Jesús A; Escandón, Patricia; Rodas, Juan D; Palomar, Ana M; Portillo, Aránzazu; Oteo, José A; Hidalgo, Marylin

    2016-02-01

    The aim of this work was to detect and identify Rickettsia species in ticks collected in rural areas of Villeta, Colombia. Tick specimens were collected from domestic animals and walls of houses in five rural villages of Villeta town and from humans in Naranjal village (same town). Moreover, a flea collected from the same area was also processed. DNA was extracted and tested by conventional, semi-nested, and nested PCR reactions targeting rickettsial genes. In the ticks collected from humans from Naranjal village, a nymph of Amblyomma cajennense sensu lato was amplified using primers for ompA and sequenced (100% identity with "Candidatus Rickettsia amblyommii"). Last, three amplicons from the Ctenocephalides felis flea, corresponding to gltA, ompB, and 16S rRNA genes, showed high identity with R. felis (98.5%, 97.3%, and 99.2%, respectively) and "Candidatus Rickettsia asemboensis" (99.7% and 100%, respectively). To our knowledge, these results correspond to the first molecular detection in Colombia of "Candidatus Rickettsia amblyommii" and "Ca. Rickettsia asemboensis" in fleas.

  4. Presence of Coxiella burnetii in fleas in Cyprus.

    Science.gov (United States)

    Psaroulaki, Anna; Chochlakis, Dimosthenis; Ioannou, Ioannis; Angelakis, Emmanouil; Tselentis, Yannis

    2014-09-01

    Over 40 tick species are naturally infected by Coxiella burnetii. However, little is known about the presence of C. burnetii in other ectoparasites such as fleas. During a 6-year (2000-2006) study, 1147 fleas were collected from 652 animals (252 rats, 118 foxes, and 282 hares) captured from different areas of Cyprus. Three flea species-Xenopsylla cheopis, Ctenocephalides felis, and C. canis-were identified. Fleas were pooled (153 pools) and tested by PCR for the presence of C. burnetii. The pathogen was identified in 25 (16.3%) pools. None of the fleas parasitizing hares was positive for C. burnetii, as opposed to fleas collected from rats (12% pool positivity) and foxes (47.6% pool positivity). The highest prevalence of positive pools was recorded in C. canis (38%) compared to C. felis (16.6%) and X. cheopis (10.8%). All pools of C. canis positive for C. burnetii were removed from foxes (44.4%), whereas all positive X. cheopis (10.8%) were removed from rats. The role of fleas in the maintenance and transmission of C. burnetii among wild vertebrates remains to be determined.

  5. Molecular identification of Cryptosporidium spp. in seagulls, pigeons, dogs, and cats in Thailand

    Directory of Open Access Journals (Sweden)

    Koompapong Khuanchai

    2014-01-01

    Full Text Available Zoonotic Cryptosporidium spp., particularly C. meleagridis, C. canis, and C. felis, are enteric protozoa responsible for major public health concerns around the world. To determine the spread of this parasite in Thailand, we conducted molecular identification of Cryptosporidium spp. from animal samples around the country, by collecting and investigating the feces of seagulls (Chroicocephalus brunnicephalus and Chroicocephalus ridibundus, domestic pigeons (Columba livia domestica, dogs, and cats. Seagull and pigeon samples were collected at the seaside and on the riverside to evaluate their potential for waterborne transmission. Ten pigeon samples were combined into one set, and a total of seven sets were collected. Seventy seagull samples were combined into one set, and a total of 13 sets were collected. In addition, 111 dog samples were collected from cattle farms, and 95 dog and 80 cat samples were collected from a temple. We identified C. meleagridis in pigeons, Cryptosporidium avian genotype III in seagulls, C. canis in dogs, and C. felis in cats. In the temple, the prevalence was 2.1% (2/95 for dogs and 2.5% (2/80 for cats. No Cryptosporidium was found in dog samples from cattle farms. These are the first findings of C. meleagridis in domestic pigeons, and Cryptosporidium avian genotype III in seagulls. Our study invites further molecular epidemiological investigations of Cryptosporidium in these animals and their environment to evaluate the public health risk in Thailand.

  6. Molecular detection of tick-borne protozoan parasites in a population of domestic cats in midwestern Brazil.

    Science.gov (United States)

    Braga, Ísis Assis; de Souza Ramos, Dirceu Guilherme; Marcili, Arlei; Melo, Andréia Lima Tomé; Taques, Isis Indaiara Gonçalves Granjeiro; Amude, Alexandre Mendes; Chitarra, Cristiane Silva; Nakazato, Luciano; Dutra, Valéria; de Campos Pacheco, Richard; Aguiar, Daniel Moura

    2016-07-01

    Some tick-borne pathogens that infect domestic cats have been considered emergent in veterinary medicine. Occurrences of Hepatozoon spp., Babesia spp. and Cytauxzoon spp. have been described in several regions of Brazil. This paper offers a comprehensive analysis of the 18S rRNA gene of a Hepatozoon sp. strain detected in domestic cats in the metropolitan area of Cuiabá, in Midwestern Brazil. Based on a molecular analysis, we detected the presence of Hepatozoon species circulating among cats in this region. The aforementioned strain is closely related to other isolates of H. felis detected in wild felids. Moreover, a phylogenetic analysis indicates that this genotype is grouped into a clade of 18S rRNA sequences previously described for the genus Hepatozoon in wild felids around the world. Hepatozoon felis strains detected in cats from Spain and Israel showed, respectively, 98% and 97% identity to our sequence and are clustered on a separate branch of the phylogenetic tree. This finding suggests a high diversity of Hepatozoon genotypes occurring in cats in Europe and South America. None of the analyzed cats were positive for Babesia spp. or Cytauxzoon spp. by PCR analysis.

  7. Mapping trends of large and medium size carnivores of conservation interest in Romania

    Directory of Open Access Journals (Sweden)

    Mihai Cristian Adamescu

    2014-07-01

    Full Text Available We analysed yearly estimates of population size data during 2001-2012 for five carnivores species of conservation interest (Ursus arctos, Canis lupus, Lynx lynx, Felis silvestris and Canis aureus. Population size estimations were done by the game management authorities and integrated by the competent authorities on the Ministry of Environment and Climate Change. Trends in data were detected using non-parametric Mann-Kendall test. This test was chosen considering the short length of data series and its usefulness for non-normal distributed data. The trend was tested at three spatial scales: game management units (n=1565, biogeographical region (n=5 and national. Trends depicted for each game management unit were plotted using ArcGIS, resulting species trend distribution maps. For the studied period increasing population trends were observed for Ursus arctos, Canis lupus, Canis aureus and Lynx lynx, while for Felis silvestris there was no trend recorded. Such an analysis in especially useful for conservation proposes, game management and reporting obligations under article 17 of the EC Habitat Directive, using population trend as a proxy for population dynamics. We conclude that the status of the five carnivore species is favourable during the study period.

  8. Molecular Detection of Bartonella Species in Fleas Collected from Dogs and Cats from Costa Rica.

    Science.gov (United States)

    Rojas, Norman; Troyo, Adriana; Castillo, Daniela; Gutierrez, Ricardo; Harrus, Shimon

    2015-10-01

    The bacterial genus Bartonella includes several species with zoonotic potential, some of which are common in domestic dogs and cats, as well as in their fleas. Because there is no previous information about the presence of Bartonella species in fleas from Central America, this study aimed at evaluating the presence of Bartonella spp. in fleas collected from dogs and cats in Costa Rica. A total 72 pools of Ctenocephalides felis and 21 pools of Pulex simulans were screened by conventional PCR to detect Bartonella DNA fragments of the citrate synthase (gltA) and the β subunit RNA polymerase (rpoB) genes. Three (4.2%) pools of C. felis and five pools (22.7%) of P. simulans were found positive for Bartonella DNA. Sequences corresponding to Bartonella vinsonii subsp. berkhoffii strain Winnie, B. rochalimae, and an undescribed Bartonella sp. (clone BR10) were detected in flea pools from dogs, whereas Bartonella henselae and B. clarridgeiae sequences were identified in flea pools from cats. The detection of zoonotic Bartonella spp. in this study should increase the awareness to these flea-borne diseases among physicians and public health workers and highlight the importance of flea control in the region.

  9. Prevalence of Rickettsia and Bartonella species in Spanish cats and their fleas.

    Science.gov (United States)

    Gracia, María Jesús; Marcén, José Miguel; Pinal, Rocio; Calvete, Carlos; Rodes, Daniel

    2015-12-01

    The aim of this study was to determine the prevalence of Bartonella henselae, Rickettsia felis, and Rickettsia typhi in fleas and companion cats (serum and claws) and to assess their presence as a function of host, host habitat, and level of parasitism. Eighty-nine serum and claw samples and 90 flea pools were collected. Cat sera were assayed by IFA for Bartonella henselae and Rickettssia species IgG antibodies. Conventional PCRs were performed on DNA extracted from nails and fleas collected from cats. A large portion (55.8%) of the feline population sampled was exposed to at least one of the three tested vector-borne pathogens. Seroreactivity to B. henselae was found in 50% of the feline studied population, and to R. felis in 16.3%. R. typhi antibodies were not found in any cat. No Bartonella sp. DNA was amplified from the claws. Flea samples from 41 cats (46%) showed molecular evidence for at least one pathogen; our study demonstrated a prevalence rate of 43.3 % of Rickettsia sp and 4.4% of Bartonella sp. in the studied flea population. None of the risk factors studied (cat's features, host habitat, and level of parasitation) was associated with either the serology or the PCR results for Bartonella sp. and Rickettsia sp.. Flea-associated infectious agents are common in cats and fleas and support the recommendation that stringent flea control should be maintained on cats.

  10. Prevalence of Bartonella species, haemoplasmas and Toxoplasma gondii in cats in Scotland.

    Science.gov (United States)

    Bennett, Alexander D; Gunn-Moore, Danielle A; Brewer, Melissa; Lappin, Michael R

    2011-08-01

    The objective of this study was to determine the prevalence rates for select infectious agents of cats presented to the Royal (Dick) School of Veterinary Studies at the University of Edinburgh, Scotland. Whole blood, serum, and oral mucosal and nail bed swabs were collected. While Ehrlichia species, Anaplasma species or Rickettsia felis DNA were not amplified from any cat, 44.2% of the cats had evidence of infection or exposure to either a Bartonella species (15.3% were seropositive and 5.8% polymerase chain reaction (PCR) positive), a haemoplasma (28.6% PCR positive), and/or Toxoplasma gondii (19.2% seropositive). No Bartonella species DNA was amplified from the nail or oral mucosal swabs despite a 5.8% amplification rate from the blood samples. This finding likely reflects the absence of Ctenocephalides felis infection from our study population, as this organism is a key component for Bartonella species translocation in cats. The results from this study support the use of flea control products to lessen exposure of cats (and people) to Bartonella species and support discouraging the feeding of raw meat to cats and preventing them from hunting to lessen T gondii infection.

  11. N-acetylcysteine, a novel treatment for Helicobacter pylori infection.

    Science.gov (United States)

    Huynh, Hien Quoc; Couper, Richard T L; Tran, Cuong D; Moore, Lynette; Kelso, Richard; Butler, Ross N

    2004-01-01

    N-Acetylcysteine (NAC), being both a mucolytic agent and a thiol-containing antioxidant, may affect the establishment and maintenance of H. pylori infection within the gastric mucus layer and mucosa. Agar and broth dilution susceptibility tests determined the MIC of H. pylori strain SSI to NAC. H. pylori load in SSI strain-infected C57BL mice was determined as colony forming units per gram of gastric tissue. Gastritis assessment was scored and gastric surface hydrophobicity was determined by contact angle measurement. MICs of NAC were 5 to 10 and 10 to 15 mg/ml using the agar dilution and broth dilution methods, respectively. NAC (120 mg per day for 14 days) reduced the H. pylori load in mice by almost 1 log compared with sham treatment. Pretreatment with NAC (40 mg/day) also significantly reduced the H. pylori load but did not prevent H. pylori colonization. Both H. pylori infection and NAC reduced the surface hydrophobicity of murine gastric mucosa. No significant differences were observed in the gastritis scores of H. felis- or H. pylori-infected mice receiving either NAC or sham treatments. This study demonstrates that NAC inhibits the growth of H. pylori in both agar and broth susceptibility tests and in H. pylori-infected mice. NAC did not alter the severity of H. pylori- or H. felis-induced gastritis.

  12. Changes in Mediterranean mesocarnivore communities along urban and ex-urban gradients

    Institute of Scientific and Technical Information of China (English)

    Mariano R RECIO; Carmen M ARIJA; Sara CABEZAS-DAZ; Emilio VIRGS

    2015-01-01

    Urbanization causes wildlife habitat loss, fragmentation, and the replacement of specialist species by generalists and/or exotic taxa. Because mesocarnivores are particularly vulnerable to habitat modifications, the rapid expansion of urban areas and the increasing trend for ex-urban development occurring in Mediterranean ecosystems may be major drivers of change in mesocarnivore communities. We combined camera trapping and sign surveys to quantify the richness and relative abundance of a set of wild and domestic mesocarnivores. We quantified these variables controlling for the gradient of urbanism, ex-urbanism, and other environmental variables in patches of natural vegetation in the region of Madrid (central Spain), and a non-urbanized control area ~220 km south of Madrid city. Using conditional autoregressive models (CAR) and model selection procedures, we found that urbanization influenced mesocarnivore community composition but this influence was not detrimental for all the species tested. Generalist carnivores such as the red foxVulpes vulpes were more abundant in urban and ex-urban areas. Ex-urban development creates overlapping areas between wild and domestic species (such as the domestic catFelis catus and the wildcat Felis silvestris) but contact between wild and domestic carnivores in natural areas is unlikely. Detection of species in the control area was very low. Therefore, the impact of urbanization in causing changes in mesocarnivore communities may be less than other factors such as illegal predator culling [Current Zoology 61 (5): 793–801, 2015].

  13. Arthropod parasites of Nubian ibexes (Capra ibex nubiana) and gazelles (Gazella gazella) in Israel.

    Science.gov (United States)

    Yeruham, I; Rosen, S; Hadani, A; Braverman, Y

    1999-06-15

    In a 20-year survey the following ectoparasites were collected from Nubian ibexes: larvae of an unidentified Oestrus sp. collected from the nasal cavities, sinuses and horns, hippoboscid flies (Lipoptena chalcomelaena) specific to the Nubian ibex, blood sucking lice (Linognathus africanus) and unidentified biting lice (Damalinia sp.). Ibexes were severely infested with the cattle tick, Boophilus annulatus; a few Hyalomma anatolicum excavatum ticks were also collected. In five ibexes kept in two zoos, showing otitis, Psoroptes cuniculi, was identified, and from skin scrpaings of nine animals with severe dermatitis in three other zoos, Sarcoptes scabiei was isolated. Infestation of Nubian ibexes with sucking and biting lice as well as H. anatolicum excavatum is reported for the first time. The ectoparasites collected from gazelles were: hippoboscid flies (Lipoptena capreoli), calliphorid flies (Lucilia sericata and Calliphora sp.), sucking lice (Linognathus africanus and Solenopotes capillatus) and unidentified biting lice (Damalinia sp.), fleas (Ctenocephalides felis felis), and ticks, B. annulatus, Rhipicepahlus bursa, Rhipicephalus turanicus, H. anatolicum excavatum and H. marginatum rufipes. In skin scrapings of four gazelles with local dermatitis in the fetlocks Chorioptes bovis was identified. Neoschoengastia sp. was found in craters between the claws in three gazelles.

  14. Molecular identification of Cryptosporidium spp. in seagulls, pigeons, dogs, and cats in Thailand.

    Science.gov (United States)

    Koompapong, Khuanchai; Mori, Hirotake; Thammasonthijarern, Nipa; Prasertbun, Rapeepun; Pintong, Ai-rada; Popruk, Supaluk; Rojekittikhun, Wichit; Chaisiri, Kittipong; Sukthana, Yaowalark; Mahittikorn, Aongart

    2014-01-01

    Zoonotic Cryptosporidium spp., particularly C. meleagridis, C. canis, and C. felis, are enteric protozoa responsible for major public health concerns around the world. To determine the spread of this parasite in Thailand, we conducted molecular identification of Cryptosporidium spp. from animal samples around the country, by collecting and investigating the feces of seagulls (Chroicocephalus brunnicephalus and Chroicocephalus ridibundus), domestic pigeons (Columba livia domestica), dogs, and cats. Seagull and pigeon samples were collected at the seaside and on the riverside to evaluate their potential for waterborne transmission. Ten pigeon samples were combined into one set, and a total of seven sets were collected. Seventy seagull samples were combined into one set, and a total of 13 sets were collected. In addition, 111 dog samples were collected from cattle farms, and 95 dog and 80 cat samples were collected from a temple. We identified C. meleagridis in pigeons, Cryptosporidium avian genotype III in seagulls, C. canis in dogs, and C. felis in cats. In the temple, the prevalence was 2.1% (2/95) for dogs and 2.5% (2/80) for cats. No Cryptosporidium was found in dog samples from cattle farms. These are the first findings of C. meleagridis in domestic pigeons, and Cryptosporidium avian genotype III in seagulls. Our study invites further molecular epidemiological investigations of Cryptosporidium in these animals and their environment to evaluate the public health risk in Thailand.

  15. Human rickettsioses in the Batna area, eastern Algeria.

    Science.gov (United States)

    Mokrani, Khamsa; Tebbal, Soraya; Raoult, Didier; Fournier, Pierre-Edouard

    2012-12-01

    In order to investigate the prevalence of rickettsioses in febrile exanthemas in eastern Algeria, we conducted a prospective serological analysis of all patients presenting with this clinical picture at the Infectious Diseases Department in the Batna Hospital from January 2000 to September 2006. One hundred and eight adult patients were included in the study, 46% of whom younger than 25 years, and 72.5% were admitted from May to September. Patients were tested for antibodies to Rickettsia conorii conorii, R. conorii israelensis, R. aeschlimannii, R. felis, R. sibirica mongolitimonae, R. africae, R. massiliae, R. typhi, and R. prowazekii using microimmunofluorescence and Western blot as confirmation procedure. Both methods confirmed the diagnosis of Mediterranean spotted fever in 5 patients (4.6%), flea spotted fever in 2 patients (1.9%), and R. aeschlimannii infection in 2 patients (1.9%). In addition, 4 patients were diagnosed as having murine typhus (3.7%) and one with epidemic typhus (0.9%). To the best of our knowledge, this is the first report of R. felis infection and R. aeschlimannii infection in Algeria. In conclusion, at least 5 different kinds of rickettsiosis coexist in eastern Algeria and, being responsible for 13% of cases of febrile exanthemas, should be considered among major causes of this clinical picture in this area. In addition, we emphasize the high incidence of typhus group rickettioses, including epidemic typhus, in this area.

  16. Parasites of domestic and wild animals in South Africa. XXII. Ixodid ticks on domestic dogs and on wild carnivores.

    Science.gov (United States)

    Horak, I G; Guillarmod, A J; Moolman, L C; de Vos, V

    1987-12-01

    Ixodid ticks were collected from 4 dogs on smallholdings near Grahamstown, eastern Cape Province, on 1 or more occasions each week for periods ranging from 9-36 months. Fourteen tick species were recovered and the seasonal abundance of adult Haemaphysalis leachi and adult Rhipicephalus simus was determined. Complete collections of ticks were made from 50 caracals (Felis caracal) in the Cradock, Graaff-Reinet and Southwell regions in the eastern Cape Province. The animals from Cradock and Graaff-Reinet harboured 13 ixodid tick species. The caracals from Southwell were infested with 11 tick species and the seasonal abundance of Ixodes pilosus on these animals was determined. A small-spotted genet (Genetta genetta), 1 bat-eared fox (Otocyon megalotis), 1 aardwolf (Proteles cristatus) and 6 black-backed jackal (Canis mesomelas) from various localities in the eastern Cape Province were examined for ticks and 9 species were collected. Complete tick collections were made from a side-striped jackal (Canis adustus), 2 wild dogs (Lycaon pictus), a spotted hyaena (Crocuta crocuta), a several (Felis serval), 2 African civets (Civettictis civetta), 2 leopards (Panthera pardus) and a lion (Panthera leo) in the Kruger National Park in the north-eastern Transvaal. Twelve ixodid tick specis were recovered from these animals.

  17. Imidacloprid/moxidectin topical solution for the prevention of heartworm disease and the treatment and control of flea and intestinal nematodes of cats.

    Science.gov (United States)

    Arther, R G; Charles, S; Ciszewski, D K; Davis, W L; Settje, T S

    2005-10-24

    Sixteen controlled laboratory studies, involving 420 kittens and cats, were conducted to evaluate the efficacy and safety of topically applied formulations of imidacloprid and moxidectin for the prevention of feline heartworm disease, treatment of flea infestations and treatment and control of intestinal nematodes. Unit-dose applicators and the dosing schedule used in these studies were designed to provide a minimum of 10mg imidacloprid and 1mg moxidectin/kg. Treatments were applied topically by parting the hair at the base of the skull and applying the solution on the skin. Imidacloprid treatment alone did not display activity against Dirofilaria immitis or intestinal nematodes and moxidectin treatment alone provided little or no activity against adult Ctenocephalides felis infestations. The formulation containing 10% imidacloprid and 1% moxidectin was 100% efficacious against the development of adult D. immitis infections when cats were treated 30 days after inoculation with third-stage larvae. A single treatment with this formulation also provided 88.4-100% control of adult C. felis for 35 days. Imidacloprid/moxidectin was 100% efficacious against adult Toxocara cati and 91.0-98.3% efficacious against immature adults and fourth-stage T. cati larvae. The formulation provided 98.8-100% efficacy against adult Ancylostoma and immature adults and third-stage A. tubaeforme larvae. Monthly topical application with 10% imidacloprid/1% moxidectin is convenient, efficacious and safe for the prevention of feline heartworm disease, treatment of flea infestation and for the treatment and control of intestinal nematode infections of cats.

  18. Integrative taxonomy at work: DNA barcoding of taeniids harboured by wild and domestic cats.

    Science.gov (United States)

    Galimberti, A; Romano, D F; Genchi, M; Paoloni, D; Vercillo, F; Bizzarri, L; Sassera, D; Bandi, C; Genchi, C; Ragni, B; Casiraghi, M

    2012-05-01

    In modern taxonomy, DNA barcoding is particularly useful where biometric parameters are difficult to determine or useless owing to the poor quality of samples. These situations are frequent in parasitology. Here, we present an integrated study, based on both DNA barcoding and morphological analysis, on cestodes belonging to the genus Taenia, for which biodiversity is still largely underestimated. In particular, we characterized cestodes from Italian wildcats (Felis silvestris silvestris), free-ranging domestic cats (Felis silvestris catus) and hybrids populations. Adult taeniids were collected by post-mortem examinations of the hosts and morphologically identified as Taenia taeniaeformis. We produced cox1 barcode sequences for all the analysed specimens, and we compared them with reference sequences of individuals belonging to the genus Taenia retrieved from GenBank. In order to evaluate the performance of a DNA barcoding approach to discriminate these parasites, the strength of correlation between species identification based on classical morphology and the molecular divergence of cox1 sequences was measured. Our study provides clear evidence that DNA barcoding is highly efficient to reveal the presence of cryptic lineages within already-described taeniid species. Indeed, we detected three well-defined molecular lineages within the whole panel of specimens morphologically identified as T. taeniaeformis. Two of these molecular groups were already identified by other authors and should be ranked at species level. The third molecular group encompasses only samples collected in Italy during this study, and it represents a third candidate species, still morphologically undescribed.

  19. Cat, cougar, and jaguar spermatogenesis: a comparative analysis

    Directory of Open Access Journals (Sweden)

    Deiler Sampaio Costa

    2006-09-01

    Full Text Available This work presents a comparative review about the spermatogenic process in cats (Felis domestica, jaguars (Panthera onca and cougars (Puma concolor, with emphasis on testicular biometry, gonadossomatic index, volumetric proportion of testicular parenchyma components, tubular diameter, seminiferous epithelial height and seminiferous tubule length. It was an approach of the differences among the cell proportions that allowed conclusions about the overall yield of spermatogenic process and Sertoli cell index in three feline species.O processo espermatogênico do gato doméstico (Felis domestica, da onça-pintada (Panthera onca e da onça-parda (Puma concolor são analisados de forma comparativa, dando-se ênfase à biometria testicular, ao índice gonadossomático, à proporção volumétrica dos constituintes do parênquima testicular, ao diâmetro tubular, à altura do epitélio seminífero e ao comprimento dos túbulos seminíferos. Abordam-se ainda as diferenças entres as razões celulares que permitem conclusões sobre o rendimento do processo espermatogênico e índices de células de Sértoli das três espécies.

  20. Parásitos en perros de San Juan Bautista, Isla Robinson Crusoe, Chile Parasites in dogs from San Juan Bautista, Robinson Crusoe Island, Chile

    Directory of Open Access Journals (Sweden)

    D González-Acuña

    2008-01-01

    Full Text Available Se estudió la fauna parasitaria de 40 perros en el poblado de San Juan Bautista, Isla Robinson Crusoe, Chile. El 50% (n = 20 de los perros presentó huevos de tipo Ancylostomideos, Strongyloideos y/o de especies Isospora sp. y Cystoisospora canis. No se encontraron muestras positivas a cestodos. El 100% de los perros presentó alguno de los siphonapteros Ctenocephalides canis, C. felis y/o Pulex irritans. En un perro se aisló un ejemplar de la garrapata café del perro Rhipicephalus sanguineus (2,5%. Se discute la importancia de los presentes resultados.The parasitological fauna of 40 dogs was studied in San Juan Bautista, Robinson Crusoe Island, Chile. 50% (n = 20 of the dogs had eggs of the Ancylostomid, Strongylid type and/or the oocysts of Isospora sp. and Cystoisospora canis. No positive samples of cestodes were found. 100% of the dogs were parasited by the fleas Ctenocephalides canis, C. felis and/or Pulex irritans. One brown dog tick, Rhipicephalus sanguineus, was found in one dog (2.5%. The importance of these findings is discussed.

  1. Phylogeography of the Neotropical catfish Pimelodus albicans (Siluriformes: Pimelodidae from río de la Plata basin, South America, and conservation remarks

    Directory of Open Access Journals (Sweden)

    Julia Vergara

    2008-03-01

    Full Text Available Pimelodus albicans Valenciennes, 1840 (common name "moncholo" or "bagre blanco" is an endemic species of the family Pimelodidae in the río de la Plata basin. Phylogenetic approach based on cytochrome b sequences was performed to test the existence of a unique evolutionary lineage in P. albicans and to discriminate populations units or subpopulations related to a migration behavior of this taxon in the río de la Plata basin. This study included 34 samples of P. albicans of different collecting sites in the río de la Plata estuary and in the río Arrecifes belonging to the río Paraná basin. Among 614 base pairs in the cytochrome b sequence data set, 203 were variable and 120 were phylogenetically informative sites in P. albicans. A total of twenty haplotypes, nucleotide diversity (p = 0.032 and haplotype diversity = 0.941 were found. Tajima's test showed significant value D= -1.88 (pPimelodus albicans Valenciennes, 1840 (popularmente conhecida como moncholo ou bagre branco é uma espécie endêmica da família Pimelodidae na bacia do rio da Prata. Estudos filogeográficos baseados nas seqüências do citocromo b mitocondrial foram realizados para testar a existência de uma única linhagem evolutiva in P. albicans e para discriminar unidades populacionais relacionadas ao comportamento migratório desse táxon na bacia do rio da Prata. Um total de 34 amostras de P. albicans provenientes de diferentes lugares de coleta no estuário do rio da Prata e rio Arrecifes na bacia do rio Paraná foram analisados. Entre as 614 pares de bases do citocromo b no conjunto de dados, 203 deles variaram e 120 foram sítios filogeneticamente informativos para P. albicans. No presente estudo foi encontrado um total de vinte haplótipos, diversidade de nucleotídeos (p = 0,032 e diversidade de haplótipos = 0,941. O teste de Tajima mostrou valores significativos D= -1,88 (p<0,05 rejeitando a hipótese de mutação neutra para os dados de P. albicans. Todas as an

  2. Therapeutic efficacy test in malaria falciparum in Antioquia, Colombia

    Directory of Open Access Journals (Sweden)

    Álvarez Tania

    2006-02-01

    Full Text Available Abstract Objective Evaluate the frequency of failure of eight treatments for non-complicated malaria caused by Plasmodium falciparum in patients from Turbo (Urabá region, El Bagre and Zaragoza (Bajo Cauca region, applying the 1998 protocol of the World Health Organization (WHO. Monotherapies using chloroquine (CQ, amodiaquine (AQ, mefloquine (MQ and sulphadoxine-pyrimethamine (SP, and combinations using chloroquine-sulphadoxine-pyrimethamine (CQ-SP, amodiaquine-sulphadoxine-pyrimethamine (AQ-SP, mefloquine-sulphadoxine-pyrimethamine (MQ-SP and artesunate-sulphadoxine-pyrimethamine (AS-SP, were examined. Methodology A balanced experimental design with eight groups. Samples were selected based on statistical and epidemiological criteria. Patients were followed for 21 to 28 days, including seven or eight parasitological and clinical evaluations, with an active search for defaulting patients. A non-blinded evaluation of the antimalarial treatment response (early failure, late failure, adequate response was performed. Results Initially, the loss of patients to follow-up was higher than 40%, but the immediate active search for the cases and the monetary help for transportation expenses of patients, reduced the loss to 6%. The treatment failure was: CQ 82%, AQ 30%, MQ 4%, SP 24%, CQ-SP 17%, AQ-SP 2%, MQ-S-P 0%, AS-SP 3%. Conclusion The characteristics of an optimal epidemiological monitoring system of antimalarial treatment response in Colombia are discussed. It is proposed to focus this on early failure detection, by applying a screening test every two to three years, based on a seven to 14-day follow-up. Clinical and parasitological assessment would be carried out by a general physician and a field microscopist from the local hospital, with active measures to search for defaulter patients at follow-up.

  3. Línea de base para evaluar el impacto de una planta de celulosa en el Río Uruguay

    Directory of Open Access Journals (Sweden)

    Carlos Saizar

    2011-05-01

    Full Text Available Como parte del proceso de evaluación del impacto ambiental ante la puesta en funcionamiento de la planta de pulpa de celulosa Kraft Botnia (actual UPM, se realizaron estudios de línea de base entre los años 2005 y 2007. El diseño de muestreo incluyó tres transectas perpendiculares a la línea de costa, en tres sitios: Nuevo Berlín, Fray Bentos y Las Cañas. Se realizaron estudios de parámetros físico-químicos del agua (nutrientes, sustancias orgánicas, metales, compuestos orgánicos adsorbibles (AOX, dioxinas y furanos, materia orgánica y granulometría del sedimento, estudios biológicos de las comunidades planctónicas, zoobentónicas e ícticas y bioacumulación de dioxinas en peces. El fósforo en la columna de agua presentó en promedio valores por encima de lo recomendado por la normativa nacional, mientras los contaminantes orgánicos (AOX, clorofenoles, ácidos resínicos, fitosteroles, dioxinas y furanos se mantuvieron por debajo de los valores guía recomendados a nivel internacional. Las comunidades planctónicas variaron significativamente entre muestreos, la de peces entre sitios y muestreos, mientras que la de bentos no presentó variaciones significativas espaciales ni temporales. El bagre trompudo (Iheringichthys labrosus es propuesto como especie a sermonitoreada debido a su ubicuidad y abundancia. Este trabajo ofrece una visión general de los estudios de línea de base, destacando la multiplicidad y la periodicidad de los parámetros en dos años de estudio.

  4. Malaria vivax en niños: aspectos clínicos y respuesta a la cloroquina.

    Directory of Open Access Journals (Sweden)

    Jaime Carmona Fonseca

    2009-12-01

    Full Text Available Introducción: En Colombia, no hay información actualizada sobre malaria vivax en niños. Objetivo: Describir el cuadro clínico-paraclínico de la enfermedad y evaluar la eficacia de la cloroquina (dosis estándar para curar el ataque agudo de paludismo vivax en niños (4-10 años. Metodología: Diseño experimental, balanceado, no ciego; 82 pacientes, residentes en Turbo o El Bagre. Seguimiento, 30 días. Resultados: Los síntomas y signos concordaron con la literatura. Hubo desnutrición de largo plazo en 62%, anemia en 53%, retinol bajo (19 μg/dl, recuento leucocitario normal, pruebas hepáticas y creatinina normales. A los 25-28 días postratamiento todas las alteraciones, excepto la desnutrición, habían desaparecido. Falla de la cloroquina: dos posibles fallas tardías. Proporción de falla según tres técnicas de análisis según la intención de tratar: 2.4% (0 a 24%, según el protocolo 2.6% (0 a 25%, según el peor escenario 7.3% (0 a 29%. Conclusión: El cuadro clínico-paraclínico fue similar al de los adultos. El paludismo, más que la desnutrición, fue responsable de las alteraciones clínicas-paraclínicas. La cloroquina sola, sin primaquina, resultó altamente eficaz para el ataque agudo de malaria vivax en niños y debe conservarse como primera opción terapéutica.

  5. Ciclo reprodutivo de Parauchenipterus striatulus (Pisces - Auchenipteridae na represa de Ribeirão das Lajes - RJ

    Directory of Open Access Journals (Sweden)

    Araújo F.G.

    2000-01-01

    Full Text Available Este trabalho teve como objetivo avaliar o ciclo reprodutivo do bagre de água doce Parauchenipterus striatulus, na represa de Ribeirão das Lajes, Rio de Janeiro. Foram descritos aspectos morfológicos dos estádios de maturação gonadal bem como variações no índice gonadossomático, relação peso:comprimento, fator de condição e condição gonadal. A amostragem dos peixes foi feita bimestralmente entre abril de 1996 e maio de 1997 com o uso de redes de espera de 100m de comprimento, 4m de altura e com malha entre 25 e 65mm de distância entrenós. Os 339 indivíduos capturados ao longo do período de amostragem corresponderam a 40% do total de peixes. Cinco estádios de maturação gonadal foram determinados por meio de análise macroscópica para ambos os sexos: imaturo, maturação I, maturação II, maduro, desovado (fêmeas e esvaziado (machos. O índice gonadossomático foi mais elevado entre outubro e março para ambos os sexos, sugerindo amplo período de desova enquanto o fator de condição atingiu seu máximo entre fevereiro e junho, mostrando uma relação inversa entre essas duas características. A relação peso-comprimento para machos foi de W= 0,0095L3,0862 e fêmeas de W= 0,0116L3,0126, não tendo sido encontradas diferenças significativas entre os coeficientes de alometria para ambos os sexos.

  6. VICHADA: LA HOSPITALIDAD DEL ORINOCO. Pag. 150-157

    Directory of Open Access Journals (Sweden)

    Wilmer Velandia

    2013-03-01

    Full Text Available Donde el cielo y la sabana se unen en el infinito, se vislumbra una sola montaña en la lejanía, parece un gigante, antiguo y oscuro al final de la inmensidad un “Tepuye”, después de unas cuantas horas de camino se acerca este gran anciano que data del precámbrico, pero no está solo. Se observan algunos compañeros iguales a él, se podrían considerar como los guardianes del imponente y hermoso Orinoko, considerado en lengua Sikuani como la gran serpiente enroscada acompañada de una gran riqueza paisajística cultural y biodiversa; bosques de galería, diferentes tipos de sabana, esteros, lagunas, morichales, bosques inundables, las que recorren aguas blancas, negras y mixtas, rodeadas por diferentes etnias y grupos indígenas como Piaroas, Sikuanis – Guahibos, Cuibas y Amoruas, que viven en forma pacífica con llaneros y colonos, orgullosos de estas tierras, amables y hospitalarios que desde hace muchos años comparten este paraíso con: bagres, pirañas, anguilas, rayas, arawanas, anacondas, ranas, sapos, caimanes, babos, iguanas, tortugas, loros, búhos, guacamayas, azucareros, pavones, paujiles, águilas, zamuros, aulladores, armadillos, ocarros, chigüiros, osos hormigueros, ocelotes, jaguares, nutrias, murciélagos, cuerpo espines, zorros, venados y delfines, que pasan sus días entre; cedros, moriches, saladillos, caraños, yarumos, laureles, caimos, guaduas, guamos, alcornoques, chaparros, arepillos, palmiches y gualtes.

  7. Impact of increasing market access on a tropical small-scale fishery

    Science.gov (United States)

    Stevens, Kara; Irwin, Brian J.; Kramer, Daniel; Urquhart, Gerald

    2014-01-01

    Small-scale fisheries have historically been marginalized in management and policy investments, and they often remain under-reported in national economic and fisheries statistics. Even so, small-scale fisheries are not entirely buffered from the impacts of globalization, such as the introduction and expansion of markets. This study measures the long-term impact of market-access on a coastal fishery on Nicaragua׳s remote Atlantic Coast from approximately the time when fishermen had access to stable and predictable local markets until the present, when the region has been transformed by road connection. In the last four years, fisheries trade has expanded as road connection has facilitated export to distant markets. Fishery-independent surveys were used to measure changes in indicators of fish-community status such as length-frequency, mean trophic level, and relative biomass. Species-level changes in relative biomass of common snook Centropomus undecimalis and gafftopsail catfish Bagre marinus were also evaluated since these species are the most economically valuable and likely account for the most fish biomass in the system. Using historical records, reports, current observations and interviews, changes in indicators of fishing intensity and market access over the past 17 years were assessed. From 1994 to 2011, community and species-specific metrics of the lagoon fishery declined significantly across all indicators examined. The potential social and economic outcomes of the decline in the fishery are far-reaching for the region, because this tropical fishery comprises the main source of protein and income for residents of twelve indigenous and Afro-descendent communities.

  8. Harttia merevari, a new species of catfish (Siluriformes: Loricariidae from Venezuela

    Directory of Open Access Journals (Sweden)

    Francisco Provenzano R.

    2005-12-01

    Full Text Available Harttia merevari, a new species of loricariid catfish, is described from eight specimens captured in the upper Caura River, Orinoco River basin, Venezuela. The new species is recognized by the following combination of characters: abdomen naked; two or three preanal plates; a bony plate before each branchial opening; seven lateral plates between the pectoral and pelvic fins; maxillary barbel short and attached to the oral disk by a fleshy fold; head dorsal surface and anterior portion of the body light or dark yellow with numerous, round black spots; posterior region of the body light or dark yellow with five black transverse bands, dorsal central area of the two anterior bands diffused. The discovery of this new species extends the distribution of the genus northwest to include the Orinoco River basin on the northern slope of the Guyana shield.Harttia merevari, uma espécie nova de bagre loricarideo é descrita com base em oito exemplares capturados no alto rio Caura, bacia do rio Orinoco, Venezuela. A espécie nova se distingue pela seguinte combinação de caracteres: abdome nu; duas ou três placas preanais; presença de uma placa óssea anterior a cada abertura branquial; sete placas laterais entre as nadadeiras peitorais e pélvicas; barbilhão maxilar curto e unido ao disco oral por uma dobra carnosa; superfície dorsal da cabeça e região anterior do corpo amarelo escuro ou marrom claro com numerosos pontos negros arredondados, região dorsal e posterior do corpo amarelo escuro ou marrom claro com cinco bandas transversais negras, as duas bandas anteriores com a parte dorsocentral difusa. A descoberta desta espécie nova estende a distribuição do gênero ao noroeste incluindo a bacia do rio Orinoco na vertente norte do Escudo das Guianas.

  9. Comparative study of renal sodium transport between ouabain-hypertensive rats and ouabain-nonhypertensive rats

    Institute of Scientific and Technical Information of China (English)

    GE Heng; Lü Zhuo-ren

    2006-01-01

    Objective: To compare renal sodium transport, using fractional excretions of lithium(FEii)as a marker of proximal tubule sodium reabsorption, between hypertensive and non-hypertensive ouabaintreated rats and further to elucidate the role of ouabain in pathogenesis of hypertension. Methods:Thirty male Sprague-Dawley rats weighting 180-200 g were randomly divided into normal control group and ouabain treated group. Rats were infused with 1 ml/kg · d normal saline or 27.8 μg/kg · d ouabain intraperitoneally once a day respectively. Systolic blood pressure (SBP), heart rate and body weight were recorded weekly. Rats were sacrificed 6 weeks after treatment. Blood and 24-hour urine sample were collected to measure the serum and urinary concentration of sodium, trace lithium and creatinine. Endogenous creatinine clearance rate (Ccr), fractional excretions of sodium (FENa), fractional excretions of lithium (FELi) and fractional reabsorption of sodium in the postproximal tubules (FDRNa) were calculated.Ouabain levels of plasma and renal tissue, plasma renin activity, angiotensin Ⅱ and aldosterone concentration were determined. Results: 65% of the ouabain-treated rats achieved significantly higher SBP after 4weeks, compared with that of the saline control groups or self baseline (P<0. 01). But in the other 35%of the ouabain-treated rats, their SBP was similar with control group during the experiment (P>0. 05).The body weight, heart rate and food intake between the 3 groups were no significant differences (P>0.05). FELi and FDRNa were significantly lower in ouabain-hypertensive group compared with ouabain-nonhypertensive group and control group(P<0.01 and P<0.05). The FELi and FDRNa of ouabain-nonhypertensive groups were similar with control group(P>0.05). Ccr and FENa were comparable between the 3 groups (P>0. 05). Plasma and renal tissue ouabain levels, plasma renin activity, angiotensin Ⅱ and aldosterone contents in ouabain-hypertensive rats were

  10. Caracterização da população de ectoparasitos em cães de núcleos de expansão urbana de Juiz de Fora, Minas Gerais, Brasil Characterization of ectoparasites on dogs in the nucleus of urban expansion of Juiz de Fora, Minas Gerais, Brazil

    Directory of Open Access Journals (Sweden)

    Denise F. Rodrigues

    2008-12-01

    Full Text Available No presente trabalho caracterizou-se a população de ectoparasitos em cães de núcleo de expansão urbana de Juiz de Fora, MG. As coletas foram realizadas de julho a setembro de 2003, examinando-se 101 cães SRD (sem raça definida. Os ectoparasitos foram coletados através de inspeção visual e tátil dos animais e acondicionados em frascos com etanol 70°GL e identificados sob estereoscopia. Espécimes foram clarificados e montados para análise em microscopia fotônica. Dentre os sifonápteros, Ctenocephalides felis foi mais prevalente (64,35% com intensidade média de 6,12 ± 5,37 sifonápteros/cão, seguido por Rhopalopsyllus lutzi (3,96%, híbrida C. felis X C. canis (1,98%, Tunga penetrans (1,98%. Aúnica espécie de fitiráptero encontrada foi Trichodectes canis (7,92% com 1,3 ± 0,51 fitiráptero/cão. Dentre os ixodídeos, Rhipicephalus sanguineus foi a espécie mais prevalente (49,50% com intensidade média de 6,44 ± 10,2 ixodídeos/ cão, seguido por Amblyomma cajennense (3,96%, Boophilus microplus (2,97%, A. ovale (1% e A. aureolatum (1%. As ninfas de ixodídeos foram separadas em ninfas de Amblyomminae (58,41% com 10,11 ± 10,09 ninfas/cão e ninfas Rhipicephalinae (24,75% com 2,64 ± 3,25 ninfas/cão. Em 3,96 % dos cães foram encontradas larvas de ixodídeo.The present work studied the characterization of ectoparasites on dogs in the nucleus of expansão urbana of Juiz de Fora, Minas Gerais, Brazil. The collections were conducted from July to September 2003, examining 101 dogs of no defined breed. The ectoparasites were collected through visual and tactile inspection of the animals' bodies, and were then placed in bottles with 70° GL ethanol and identified under a stereomicroscope. Specimens were clarified and mounted on slides for analysis under a photonic microscope. Among the siphonaptera, Ctenocephalides felis was the most prevalent (64.35%, with mean intensity of 6.12 ± 5.37 siphonaptera/dog, followed by Rhopalopsyllus

  11. Frequency of parasites and Salmonella infection in captive maned-wolf, Chrysocyon brachyurus, kept in Zoos at the State of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Gilioli R.

    2000-01-01

    Full Text Available Thirty-one captive maned wolves (Chrysocyon brachyurus, Illiger 1815 from 11 Zoos at the State of São Paulo, Brazil, were screened to investigate the presence of parasites and Salmonella infection by parasitological diagnostic methods and fecal selective culture. The most frequent ecto and endoparasites found were Ctenocephalides felis (56.2%, Rhipicephalus sanguineus (12.5%, Ancylostoma caninum (45.1%, Strongyloides sp. (29.0%, Uncinaria stenocephala (3.2%, Capillaria sp. (3.2%, Entamoeba sp. (22.9%, Sarcocystis sp. (29.0%, Cryptosporidium sp. (19.3%, Eimeria sp. (19.3%, Giardia sp. (9.6% and Isospora sp. (3.2%. Four different serotypes of Salmonella were identified in six animals (25%. Only one infected animal showed clinical signs of diarrhea. The ability to harbor Salmonella spp. as normal nonpathogenic bacteria of the gastrointestinal tract may be a physiological adaptation of this specie.

  12. Biochemical and immunohistochemical characterization of feline spongiform encephalopathy in a German captive cheetah.

    Science.gov (United States)

    Eiden, Martin; Hoffmann, Christine; Balkema-Buschmann, Anne; Müller, Matthias; Baumgartner, Katrin; Groschup, Martin H

    2010-11-01

    Feline spongiform encephalopathy (FSE) is a transmissible spongiform encephalopathy that affects domestic cats (Felis catus) and captive wild members of the family Felidae. In this report we describe a case of FSE in a captive cheetah from the zoological garden of Nuremberg. The biochemical examination revealed a BSE-like pattern. Disease-associated scrapie prion protein (PrP(Sc)) was widely distributed in the central and peripheral nervous system, as well as in the lymphoreticular system and in other tissues of the affected animal, as demonstrated by immunohistochemistry and/or immunoblotting. Moreover, we report for the first time the use of the protein misfolding cyclic amplification technique for highly sensitive detection of PrP(Sc) in the family Felidae. The widespread PrP(Sc) deposition suggests a simultaneous lymphatic and neural spread of the FSE agent. The detection of PrP(Sc) in the spleen indicates a potential for prion infectivity of cheetah blood.

  13. 辽宁朝阳马山洞的食肉类化石%Late Pleistocene Cave Deposits and Carnivores from the Mashandong Site,Chaoyang Municipality,Liaoning Province

    Institute of Scientific and Technical Information of China (English)

    傅仁义; 冯兴无; 张双权; 刘晓庆

    2010-01-01

    本文主要记述了辽宁省朝阳市龙城区马山洞发现的食肉类化石.堆积物的上部主要为颗粒细小的砂质黏土,而下部主要为直径较大的角砾.2007年出土的食肉目化石有:似浣熊貉(Nyctereutes procyonoides)、赤狐(Vulpes vulpes)、狗獾(Meles meles)、黄鼬(Mustela sibirica)、中华猫(Felis chinensis)和疑似虎(Panthera tigris)?.其中前5个种在形态上与现生种类非常接近;而最后一个种除与现生虎比较接近外,与现生狮子也有一定的相似性.

  14. Helminth parasites of cats from the Vientiane Province, Laos, as indicators of the occurrence of causative agents of human parasitoses

    Directory of Open Access Journals (Sweden)

    Scholz T.

    2003-12-01

    Full Text Available A total of 55 domestic cats (Felis calus f. domestico and one wild (Bengal cat (Prionailurus bengalensis from the Vientiane Province, central Laos, were examined for helminth parasites with emphasis given to potential human parasites. The following species were found (parasites infective to man marked with an asterisk: Opisthorchis viverrini*, Haplorchis pumilio*,H. laichui*,H. yokogawai*, Stellantchasmus falcatus* (Digenea; Spirometra sp.*, Dipylidium caninum*, Taenia taeniaeformis (Cestoda; Capillariidae gen. sp., Toxocara canis*, T. cati*, Ancylostoma ceylanicum*, A. tubaeforme, Gnathostoma spinigerum*, Physaloptera preputials (Nematoda; and Oncicola sp. (Acanthocephala. This study demonstrated that examination of cats may provide useful data on the occurrence of helminths which are potential causative agents of human diseases.

  15. FARS EDEBİYATI’NDA BEHLÛL HİKÂYELERİ / The Stories of Behlul in Persian Literature

    OpenAIRE

    KALKANDELEN, A.Hilâl

    2013-01-01

    ÖZ Behlûl, Kûfeli bir mecnunun ismidir. Harun Reşid'in akrabası olarak kabuledilir. İnsanlar onu "akıllı deli" diye adlandırırlar. Hakkında hikmet dolu hikâyeleranlatılır. Behlûl'ün Fars Edebiyatı'nda önemli bir yeri vardır. Pek çok şairve yazar manzum ve mensur eserlerinde ondan söz etmiş ve onunla ilgili çeşitlihikâyelere yer vermişlerdir. Bu hikâyeler çoğu zaman övgü, yergi, kınama ve nasihatşeklind...

  16. Evidence of feline immunodeficiency virus, feline leukemia virus, and Toxoplasma gondii in feral cats on Mauna Kea, Hawaii.

    Science.gov (United States)

    Danner, Raymond M; Goltz, Daniel M; Hess, Steven C; Banko, Paul C

    2007-04-01

    We determined prevalence to feline immunodeficiency virus (FIV) antibodies, feline leukemia virus (FeLV) antigen, and Toxoplasma gondii antibodies in feral cats (Felis catus) on Mauna Kea Hawaii from April 2002 to May 2004. Six of 68 (8.8%) and 11 of 68 (16.2%) cats were antibody positive to FIV and antigen positive for FeLV, respectively; 25 of 67 (37.3%) cats were seropositive to T. gondii. Antibodies to FeLV and T. gondii occurred in all age and sex classes, but FIV occurred only in adult males. Evidence of current or previous infections with two of these infectious agents was detected in eight of 64 cats (12.5%). Despite exposure to these infectious agents, feral cats remain abundant throughout the Hawaiian Islands.

  17. On harmonic operation of Shanghai deep UV free electron laser

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    By choosing parameters in the modulator,the dispersive section and the seed laser,the spatial bunching of the electron beam can be correlated to then-th harmonic of ther adiator radiation,instead of the fundamental radiation in conventional high-gain harmonic generation(HGHG).Thus,the radiator undulator is operated at high harmonic mode.In this paper,the possibility of harmonic operation of Shanghai deep ultraviolet(SDUV)free electron laser (FEL)is studied.Discussions on the principle of harmonic operation,the simulation code development,the simulation results.and the proposed experimental procedure for verification of harmonic operation at the SDUV FEL are also presented.

  18. First evidence of hemoplasma infection in free-ranging Namibian cheetahs (Acinonyx jubatus).

    Science.gov (United States)

    Krengel, Annika; Meli, Marina L; Cattori, Valentino; Wachter, Bettina; Willi, Barbara; Thalwitzer, Susanne; Melzheimer, Jörg; Hofer, Heribert; Lutz, Hans; Hofmann-Lehmann, Regina

    2013-03-23

    Infections with feline hemotropic mycoplasmas (hemoplasmas) have been documented in domestic cats and free-ranging feline species with high prevalences in Iberian lynxes (Lynx pardinus), Eurasian lynxes (Lynx lynx), European wildcats (Felis silvestris silvestris), African lions (Panthera leo) in Tanzania and domestic cats in South Africa. The prevalence of hemoplasmas has not yet been investigated in free-ranging felids in southern Africa. In this study we screened 73 blood samples from 61 cheetahs in central Namibia for the presence of hemoplasmas using quantitative real-time PCR. One of the cheetahs tested PCR-positive. Phylogenetic analysis based on partial sequencing of the 16S rRNA and RNAse P genes revealed that the isolate belongs to the Mycoplasma haemofelis/haemocanis group. This is the first molecular evidence of a hemoplasma infection in a free-ranging cheetah.

  19. Acute toxicity of copper to sea catfish

    Energy Technology Data Exchange (ETDEWEB)

    Steele, C.W.

    1983-01-01

    Sea catfish (Arius felis) were exposed to aqueous solutions of reagent grade cupric chloride in artificial seawater (30.0 +/- 2.0 0/00, 21-23/sup 0/C) in four static bioassays. The 24, 48, 72 and 96 h LC/sub 50/ were calculated and found to be 5.43, 4.17, 3.57 and 2.40 mg 1./sup -1/ copper, respectively. Experimental concentrations of copper producing subtle behavioral changes in this species correspond to less than 0.3% of the 72 h LC/sub 50/. Based on this comparison with literature values, a new, maximum 'safe' concentration for copper in marine waters of 0.01 mg 1./sup -1/ is proposed.

  20. Predation of wild spider monkeys at La Macarena, Colombia.

    Science.gov (United States)

    Matsuda, Ikki; Izawa, Kosei

    2008-01-01

    The killing of an adult male spider monkey (Ateles belzebuth ) by a jaguar (Panthera onca) and a predation attempt by a puma (Felis concolor) on an adult female spider monkey have been observed at the CIEM (Centro de Investigaciones Ecológicas La Macarena), La Macarena, Colombia. These incidents occurred directly in front of an observer, even though it is said that predation under direct observation on any type of primate rarely occurs. On the basis of a review of the literature, and the observations reported here, we suggest that jaguars and pumas are likely to be the only significant potential predators on adult spider monkeys, probably because of their large body size.

  1. 从全球案例看移动支付的关键点

    Institute of Scientific and Technical Information of China (English)

    2015-01-01

    全球移动支付最活跃的国家不是美国,而是非洲肯亚。M-PESA是一个20 07年推出的移动支付服务,有超过1 900万注册用户,占国家人口70%,每天有超过600万笔交易在平台上完成。日本可以说是移动支付领头羊,早于2004年就由NTT Docomo推出第一台Feli Ca支付手机,比苹果Apple Pay早了10年。在2011年已经有超过33%日本人使用过手机进行支付,同时间欧美只有不到15%。

  2. Small mammals of the Addo Elephant National Park

    Directory of Open Access Journals (Sweden)

    P. Swanepoel

    1975-07-01

    Full Text Available A survey of the small mammals of the Addo Elephant National Park resulted in a checklist, as well as information on relative numbers, distribution within the Park, reproductive activity, sex ratios, and body measurements. Forty mammals species occur in the Park, while three re-introduced species probably do not occur any longer. Of the 40 species 28 are considered small mammals comprising 13 rodent, eight carnivore, two shrew, two bat, one primate and one lagomorph species, as well as the aardvark: Crociduraflavescens, C. cyanea infumata, Rousettus aegyptiacus, Eptesicus capensis, Cercopithecus pygerythrus, Canis mesomelas, Ictonyx striatus, Poecilogale albinucha, Genetta sp., Herpestes pulverulentus, Suricata suricatta, Proteles cristatus, Felis caracal, Orycteropus afer, Lepus saxatilis, Cryptomys hottentotus, Hystrix africae-australis, Pedetes capensis, Graphiurus murinus, Aethomys namaquensis, Praomys natalensis, Rhabdomys pumilio, Mus minutoides, Rattus rattus, Saccostomys campestris, Desmodillus auricularis, Otomys irroratus and 0. unisulcatus.

  3. Occupancy of the Invasive Feral Cat Varies with Habitat Complexity

    Science.gov (United States)

    Hohnen, Rosemary; Tuft, Katherine; McGregor, Hugh W.; Legge, Sarah; Radford, Ian J.; Johnson, Christopher N.

    2016-01-01

    The domestic cat (Felis catus) is an invasive exotic in many locations around the world and is thought to be a key factor driving recent mammal declines across northern Australia. Many mammal species native to this region now persist only in areas with high topographic complexity, provided by features such as gorges or escarpments. Do mammals persist in these habitats because cats occupy them less, or despite high cat occupancy? We show that occupancy of feral cats was lower in mammal-rich habitats of high topographic complexity. These results support the idea that predation pressure by feral cats is a factor contributing to the collapse of mammal communities across northern Australia. Managing impacts of feral cats is a global conservation challenge. Conservation actions such as choosing sites for small mammal reintroductions may be more successful if variation in cat occupancy with landscape features is taken into account. PMID:27655024

  4. Rickettsia lusitaniae sp. nov. isolated from the soft tick Ornithodoros erraticus (Acarina: Argasidae).

    Science.gov (United States)

    Milhano, Natacha; Palma, Mariana; Marcili, Arlei; Núncio, Maria Sofia; de Carvalho, Isabel Lopes; de Sousa, Rita

    2014-05-01

    In this study a novel Rickettsia from the spotted fever group, isolated from Ornithodoros erraticus soft ticks collected from pigpens in the south of Portugal, is described. After initial screening revealed Rickettsia-positive ticks, isolation attempts were then performed. Successful isolates were achieved by shell-vial technique using Vero E6 cells at 28°C. Molecular characterization of the isolate was performed based on analysis of five rickettsial genes gltA, ompA, ompB, sca1 and htr with their subsequent concatenation along with other rickettsial species resulting in a clustering of the new isolate with Rickettsia felis and Rickettsia hoogstraalii. The degree of nucleotide sequence similarity with other rickettsiae fulfills the criteria for classification of our isolate as a novel species. The name Rickettsia lusitaniae sp. nov. (=CEVDI PoTiRo) is proposed for this new species found in O. erraticus.

  5. Molecular Detection of Fastidious and Common Bacteria as Well as Plasmodium spp. in Febrile and Afebrile Children in Franceville, Gabon.

    Science.gov (United States)

    Mourembou, Gaël; Fenollar, Florence; Socolovschi, Cristina; Lemamy, Guy Joseph; Nzoughe, Hermann; Kouna, Lady Charlene; Toure-Ndouo, Fousseyni; Million, Matthieu; Mbiguino, Angelique Ndjoyi; Lekana-Douki, Jean Bernard; Raoult, Didier

    2015-05-01

    Malaria was considered as the main cause of fever in Africa. However, with the roll back malaria initiative, the causes of fever in Africa may change. This study aimed to evaluate the prevalence of bacteria and Plasmodium spp. in febrile and afebrile (controls) children from Franceville, Gabon. About 793 blood samples from febrile children and 100 from controls were analyzed using polymerase chain reaction (PCR) coupled with sequencing. Plasmodium spp. was the microorganism most detected in febrile (74.5%, 591/793) and controls (13%, 13/100), P Gabon. Two fastidious bacteria, Rickettsia felis and Borrelia spp., were found. Inclusion of controls should improve the understanding of the causes of fever in sub-Saharan Africa.

  6. Feeding profile of Mepraia spinolai, a sylvatic vector of Chagas disease in Chile.

    Science.gov (United States)

    Chacón, F; Bacigalupo, A; Quiroga, J F; Ferreira, A; Cattan, P E; Ramírez-Toloza, G

    2016-10-01

    American trypanosomiasis is a chronic disease transmitted mainly by vectors. The hematophagous triatomine vectors transmit Trypanosoma cruzi to a wide variety of mammals, which usually are their food source. This study determined the feeding profile of Mepraia spinolai, a sylvatic triatomine vector, present in endemic areas of Chile. Vectors were captured in the north-central area of Chile. Samples of intestinal contents were analyzed by an Enzyme-linked immunosorbent assay (ELISA) that identifies and discriminates the presence of serum antigens from Homo sapiens and nine animal species (Canis familiaris, Felis catus, Capra hircus, Mus musculus, Gallus gallus, Octodon degus, Thylamys elegans, Phyllotis darwini and Oryctolagus cuniculus). Our data indicate the most frequent feeding source in this area was P. darwini, followed by O. degus, O. cuniculus, M. musculus, G. gallus, T. elegans, C. familiaris, F. catus and C. hircus. Mixed food sources were also identified.

  7. [New drugs for small animals in 2014].

    Science.gov (United States)

    Emmerich, I U

    2015-01-01

    In 2014, six active pharmaceutical ingredients were released on the German market for small animals. Those are the ektoparasiticide of the isoxazoline group afoxolaner (NexGard®) and fluralaner (Bravecto®) and the neonicotinoid dinotefuran (Vectra 3D, Vectra Felis), the antidiabetic protamine zinc insulin of human origin (ProZinc®), the antifungal agent ketoconazole (Fugazid®) as well as the cytostatic drug oclacitinib (Apoquel®). Two substances were authorized for an additional species. The antiparasiticide eprinomectin and the antibiotic clindamycin were also authorized for use in cats. In addition, two active pharmaceutical ingredients, which were approved 2014 for use in human medicine and are of potential interest to veterinary medicine, are discussed. These are the antihypertensive drug riociguat and the urological substance mirabegron.

  8. 装备零讯

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    日海上自卫队第十艘亲潮级潜艇服役;英国发射“天网”5A军事通信卫星;巴海军“阿戈斯塔”90B潜艇将安装新型推进装置;南非海军第二艘209/1400级常规潜艇服役;法军将有27个步兵团配装FELIN未来士兵系统;美国海军SSN775核攻击潜艇展开后期试航试验;印第一艘核潜艇计划于下半年下水

  9. Evidence of feline immunodeficiency virus, feline leukemia virus, and Toxoplasma gondii in feral cats on Mauna Kea, Hawaii

    Science.gov (United States)

    Danner, R.M.; Goltz, Dan M.; Hess, S.C.; Banko, P.C.

    2007-01-01

    We determined prevalence to feline immunodeficiency virus (FIV) antibodies, feline leukemia virus (FeLV) antigen, and Toxoplasma gondii antibodies in feral cats (Felis catus) on Mauna Kea Hawaii from April 2002 to May 2004. Six of 68 (8.8%) and 11 of 68 (16.2%) cats were antibody positive to FIV and antigen positive for FeLV, respectively; 25 of 67 (37.3%) cats were seropositive to T. gondii. Antibodies to FeLV and T. gondii occurred in all age and sex classes, but FIV occurred only in adult males. Evidence of current or previous infections with two of these infectious agents was detected in eight of 64 cats (12.5%). Despite exposure to these infectious agents, feral cats remain abundant throughout the Hawaiian Islands. ?? Wildlife Disease Association 2007.

  10. BrainMaps.org - Interactive High-Resolution Digital Brain Atlases and Virtual Microscopy.

    Science.gov (United States)

    Mikula, Shawn; Stone, James M; Jones, Edward G

    2008-01-01

    BrainMaps.org is an interactive high-resolution digital brain atlas and virtual microscope that is based on over 20 million megapixels of scanned images of serial sections of both primate and non-primate brains and that is integrated with a high-speed database for querying and retrieving data about brain structure and function over the internet. Complete brain datasets for various species, including Homo sapiens, Macaca mulatta, Chlorocebus aethiops, Felis catus, Mus musculus, Rattus norvegicus, and Tyto alba, are accessible online. The methods and tools we describe are useful for both research and teaching, and can be replicated by labs seeking to increase accessibility and sharing of neuroanatomical data. These tools offer the possibility of visualizing and exploring completely digitized sections of brains at a sub-neuronal level, and can facilitate large-scale connectional tracing, histochemical and stereological analyses.

  11. Y型嗅觉仪在蚤类对宿主选择性研究中的应用%Application of Y-tube olfactometer in the study of host selection behaviors of fleas

    Institute of Scientific and Technical Information of China (English)

    林杰; 孟凤霞; 吴丹丹

    2011-01-01

    Objective To establish the method for the detection of host odor selection of fleas using the Y-tube olfactometer, and to learn the host selecting behaviors of Ctenocephalides felis felis, Xenopsylla cheopis and Leptopsylla segnis between white rat and white mice, providing evidence for the study of flea host selection and the relationship of "fleas-host/human-diseases". Methods With X. cheopis used as the test insect and Y-tube olfactometer as the test device, the effect of Y-tube wind speed, the arm-tube position of the Y-tube olfactometer and test room light on the flea host selection behaviors were studied.With this method, the host selection behaviors between rats and mice of C. felis felis, X. cheopis and L. segnis laboratory populations were measured. Results It was found that in the period of detection that lasted for 20 min there was no significant differences in the selection behaviors of X. cheopis when the air flow rate was 0.6-1.5 L/min, neither were the arm-tube position of the Y-tube offactometer and the changes in light. C. felisfelis was found to have significant selectivity for rats by the established method, while X. cheopis and L. segnis had no significantly different behavior response to the rats and mice.Conclusion The host selection behaviors of fleas can be measured with Y-tube offactometer. Host selection behaviors are helpful in understanding their host specificity and in learning the correlation of "flea-host/human-diseases".%目的 应用Y型嗅觉仪,建立蚤类对宿主动物气味选择的测定方法,并研究猫栉首蚤、印鼠客蚤和缓慢细蚤对大白鼠和小白鼠的选择行为,为探究不同蚤种对不同宿主动物的特异性选择和了解"蚤类-宿主动物或人-相关传染病"的关系提供理论依据.方法 以印鼠客蚤为试虫,用Y型嗅觉仪测定风速、Y型管臂管的几何位置、光照对其选择行为的影响,以猫栉首蚤、印鼠客蚤和缓慢细蚤成蚤为试虫分别测定对大

  12. Tick and flea infestation in a captive Margay Leopardus wiedii (Schinz, 1821 (Carnivora: Felidae: Felinae in Peru

    Directory of Open Access Journals (Sweden)

    M. Quevedo

    2014-02-01

    Full Text Available Interaction between wild and domestic animals can increase the risk for transmission of parasites in both directions, and thus, affects the ecology of diseases. Wild felids have been proven to be sensitive to infectious agents commonly found in domestic animals, and those agents have had detrimental effects on wildlife conservation. A margay Leopardus wiedii which had been kept captive as a pet for about fifteen days, was found moderately infested with the brown dog tick Rhipicephalus sanguineus and the cat flea Ctenocephalides felis. Considering that the captive Margay lived close to domestic dogs and cats, this interaction might be the source of that infestation. Based on this finding, careful attention should be paid to wildlife and domestic animals interactions as ectoparasites can be easily transmitted and new host-pathogen interactions are possible.

  13. Rickettsial pathogens and arthropod vectors of medical and veterinary significance on Kwajalein Atoll and Wake Island

    Directory of Open Access Journals (Sweden)

    Durden, L.

    2012-05-01

    Full Text Available Modern surveys of ectoparasites and potential vector-borne pathogens in the Republic of the Marshall Islands and Wake Island are poorly documented. We report on field surveys of ectoparasites from 2010 with collections from dogs, cats, and rats. Five ectoparasites were identified: the cat flea Ctenocephalides felis, a sucking louse Hoplopleura pacifica, the mites Laelaps nuttalli and Radfordia ensifera, and the brown dog tickRhipicephalus sanguineus. Ectoparasites were screened for rickettsial pathogens. DNA from Anaplasma platys, a Coxiella symbiont of Rhipicephalus sanguineus, anda Rickettsia sp. were identified by PCR and DNA sequencing from ticks and fleas on Kwajalein Atoll. An unidentified spotted fever group Rickettsia was detected in a pool of Laelaps nuttalli and Hoplopleura pacifica from Wake Island. The records of Hoplopleura pacifica, Laelaps nuttalli, and Radfordia ensifera and the pathogens are new for Kwajalein Atoll and Wake Island.

  14. Auditory lateralization of conspecific and heterospecific vocalizations in cats.

    Science.gov (United States)

    Siniscalchi, Marcello; Laddago, Serena; Quaranta, Angelo

    2016-01-01

    Auditory lateralization in response to both conspecific and heterospecific vocalizations (dog vocalizations) was observed in 16 tabby cats (Felis catus). Six different vocalizations were used: cat "purring," "meowing" and "growling" and dog typical vocalizations of "disturbance," "isolation" and "play." The head-orienting paradigm showed that cats turned their head with the right ear leading (left hemisphere activation) in response to their typical-species vocalization ("meow" and "purring"); on the other hand, a clear bias in the use of the left ear (right hemisphere activation) was observed in response to vocalizations eliciting intense emotion (dogs' vocalizations of "disturbance" and "isolation"). Overall these findings suggest that auditory sensory domain seems to be lateralized also in cat species, stressing the role of the left hemisphere for intraspecific communication and of the right hemisphere in processing threatening and alarming stimuli.

  15. Ectoparasites of dogs in home environments on the Caribbean slope of Costa Rica Ectoparasitos de cães no ambiente doméstico da vertente Caribe, Costa Rica

    Directory of Open Access Journals (Sweden)

    Adriana Troyo

    2012-06-01

    Full Text Available Reports on ectoparasites on dogs in Central America are scarce. The aim of this study was to identify flea, louse and tick species infesting dogs in home environments on the Caribbean slope of Costa Rica, and determine their frequency and coexistence. Ectoparasites were collected from dogs in 83 rural homes at five study sites. Specimens were identified and separated according to species. Fleas were the most common ectoparasite (G = 22,217, DF = 8, p = 0.004. Ctenocephalides felis and Pulex simulans were found in 83% and 55% of the homes with ectoparasites, respectively. Trichodectes canis (13%, Heterodoxus spiniger (10%, Rhipicephalus sanguineus (18%, Rhipicephalus (Boophilus microplus (5% and Amblyomma ovale (8% were also present. More than one species was collected in most cases (66%, and the most common combination was C. felis and P. simulans (59% of homes with fleas. The high frequency of P. simulans emphasizes the need for adequate identification. This was the first study involving different ectoparasites of dogs in Costa Rica, as well as the first report of T. canis in this country. The relative frequency and coexistence of these ectoparasites in the home environment may have implications for animal and human health.Relatos de ectoparasitos em cães da América Central são escassos. O objetivo deste estudo foi identificar espécies de pulgas, piolhos e carrapatos que infestam os cães no ambiente doméstico da vertente Caribe da Costa Rica, determinando sua frequência e coexistência. Foram coletados ectoparasitos de cães em 83 casas rurais de cinco sítios do estudo. Os indivíduos foram separados e identificados. Os ectoparasitos mais comuns foram as pulgas (G = 22.217, DF = 8, p = 0.004. Ctenocephalides felis e Pulex simulans foram observados em 83% e 55% das moradias com ectoparasitos, respectivamente. Da mesma forma também foram observados Trichodectes canis (13%, Heterodoxus spiniger (10%, Rhipicephalus sanguineus (18

  16. Identification of Helicobacter spp. in gastrointestinal tract, pancreas and hepatobiliary system of stray cats.

    Science.gov (United States)

    Shojaee Tabrizi, A; Derakhshandeh, A; Esfandiari, A; Ali Atashi, Z

    2015-01-01

    The aim of the present study was to determine the presence of Helicobacter species in different parts of gastrointestinal tract, hepatobiliary system and pancreas of stray cats. Six different sites at the level of genus, gastric (H. heilmannii and H. felis) and enterohepatic species of Helicobacter were investigated in six cats using species-specific primers by polymerase chain reaction (PCR). Interestingly, DNA of enterohepatic spp. was detected in 1/6 duodenum, 2/6 colon and 1/6 pancreas specimens. Results of sequencing revealed that all of these four positive samples belong to Helicobacter canis. While cats have not been considered as a potential zoonotic danger for non-pylori Helicobacter infections, the results of current study show prompt re-evaluation of that view. To the best of our knowledge, this is the first study about distribution of Helicobcater spp. in gastrointestinal tract of cats.

  17. Dicty_cDB: VHD183 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available significant alignments: (bits) Value L25926_1( L25926 |pid:none) Rat sucrase-isomaltase (SI) mRNA, comp... 3...8 0.018 ( P23739 ) RecName: Full=Sucrase-isomaltase, intestinal; Contains:... 38 0.018 ( O62653 ) RecName: Full=Sucrase-isomalt...id:none) Mus musculus sucrase-isomaltase mR... 36 0.11 AB428422_1( AB428422 |pid:none) Felis catus SI mRNA f...or sucrase-is... 36 0.47 BC115034_1( BC115034 |pid:none) Homo sapiens sucrase-isomaltase (a... 35 0.81 ( P14...410 ) RecName: Full=Sucrase-isomaltase, intestinal; Contains:... 35 0.81 BC116452

  18. Host range characteristics of the primate coccidian, Isospora arctopitheci Rodhain 1933 (Protozoa: Eimeriidae).

    Science.gov (United States)

    Hendricks, L D

    1977-02-01

    Studies were conducted on 35 primates, 12 carnivores, and 2 marsupials to determine their susceptibility to the primate coccidian, Isospora arctopitheci. Patent oocyst infections resulted in 12 of the 14 species of animals investigated. These included 6 genera of New World primates native to Panama: Saguinus geoffroyi, Aotus trivirgatus, Ateles fusciceps, Cebus capucinus, Alouatta villosa, and Saimiri sciureus. In addition 4 families of carnivores (2 domestic and 2 sylvatic) and 1 species of marsupial became infected following experimental exposure. These animals are represented respectively by the following 6 genera and species: Canis familiaris; Felis catus; Nasua nasua, and Potos flavus; Eiria barbara; and Didelphis marsupialis. Four Old World rhesus monkeys, Macaca mulatta, and 1 carnivore, Bassaricyon gabbii, did not become oocyst positive. This unusually large host range makes this isosporan unique among the coccidia that have been investigated to date.

  19. Trichinella infection in wildlife of the southwestern United States.

    Science.gov (United States)

    Pozio, E; Pence, D B; La Rosa, G; Casulli, A; Henke, S E

    2001-10-01

    Several potential mammalian reservoirs of sylvatic species of Trichinella were examined from Texas, New Mexico, and Arizona. During 1998-99, tongues were collected from a black bear (Ursus americanus) in Arizona; from 9 black bears, a coyote (Canis latrans), and a mountain lion (Felis concolor) in New Mexico; and from 154 coyotes, 32 raccoons (Procyon lotor), 13 opossums (Didelphis marsupialis), 4 ocelots (Leopardus pardalis), 3 bobcats (Lynx rufus), and 5 feral hogs (Sus scrofa) in southern Texas. Larvae of Trichinella murrelli were identified by a multiple-polymerase chain reaction analysis in 1 black bear (11.1%) from New Mexico and in 7 coyotes (4.5%) of Texas, whereas Trichinella spiralis larvae were detected in the black bear of Arizona. This is the first report of Trichinella infection in wildlife of New Mexico and Texas and extends the distribution of T. murrelli into the southwestern United States near the border of Mexico.

  20. PREVALENCE OF ANTIBODIES TO SELECTED VIRUSES AND PARASITES IN INTRODUCED AND ENDEMIC CARNIVORES IN WESTERN MADAGASCAR.

    Science.gov (United States)

    Pomerantz, Julie; Rasambainarivo, Fidisoa T; Dollar, Luke; Rahajanirina, Leon Pierrot; Andrianaivoarivelo, Radosoa; Parker, Patricia; Dubovi, Edward

    2016-07-01

    Introduced animals impact endemic populations through predation, competition, and disease transmission. Populations of endemic carnivores in Madagascar are declining, and pathogens transmitted from introduced species may further endanger these unique species. We assessed the exposure of introduced and endemic carnivores to common viral and parasitic pathogens in two national parks of Madagascar (Kirindy Mitea National Park and Ankarafantsika National Park) and their neighboring villages. We also identified variables associated with the presence of antibodies to these pathogens in fosa ( Cryptoprocta ferox ). Introduced and endemic species were exposed to canine parvovirus, canine herpesvirus, feline calicivirus, and Toxoplasma gondii . Domestic dogs ( Canis familiaris ) and cats ( Felis catus ) may be sources of infection for these pathogens. Prevalence of antibodies to Toxoplasma in captured fosa was >93%, and adults were more likely to be exposed than immature individuals. Our data provide a basis upon which to evaluate and manage risks of pathogen transmission between species.

  1. Transcriptome analysis of feline infectious peritonitis virus infection.

    Science.gov (United States)

    Mehrbod, Parvaneh; Harun, Mohammad Syamsul Reza; Shuid, Ahmad Naqib; Omar, Abdul Rahman

    2015-01-01

    Feline infectious peritonitis (FIP) is a lethal systemic disease caused by FIP virus (FIPV). There are no effective vaccines or treatment available, and the virus virulence determinants and pathogenesis are not fully understood. Here, we describe the sequencing of RNA extracted from Crandell Rees Feline Kidney (CRFK) cells infected with FIPV using the Illumina next-generation sequencing approach. Bioinformatics analysis, based on Felis catus 2X annotated shotgun reference genome, using CLC bio Genome Workbench is used to map both control and infected cells. Kal's Z test statistical analysis is used to analyze the differentially expressed genes from the infected CRFK cells. In addition, RT-qPCR analysis is used for further transcriptional profiling of selected genes in infected CRFK cells and Peripheral Blood Mononuclear Cells (PBMCs) from healthy and FIP-diagnosed cats.

  2. Pathogenic role of gastric Helicobacter sp in domestic carnivores.

    Science.gov (United States)

    Lecoindre, P; Chevallier, M; Peyrol, S; Boude, M; Ferrero, R L; Labigne, A

    1997-01-01

    As a result of phylogenic studies using new molecular biology techniques and fundamental experimental studies, we now know more about helicobacteria in domestic carnivores, their morphologic characteristics, their taxonomia and more important we know more about their ecological niche. Few clinical studies have been carried out, but the ones that have been undertaken are interesting in that they confirm the extensive prevalence of Helicobacter infections in domestic carnivores and underline their role in the genesis of the inflammatory gastropathies observed in these species. Finally, recent observations have demonstrated the ubiquitous character of these helicobacteria by showing their presence in the stomach of man, dog and cat. This ubiquitous character has led some scientists to consider the potential zoonotic risk of the human infection by Helicobacter heilmannii, felis or pylori.

  3. Gastrointestinal parasites of stray cats in Kashan, Iran.

    Science.gov (United States)

    Mohsen, A; Hossein, H

    2009-04-01

    Considering the role of parasites in contamination of human beings and domestic animals and lack of information in the region, the present study was performed to investigate the infection status of helminthes and protozoa of stray cats in central Iran. A cross - sectional study was conducted on 113 stray cats trapped from different geographic regions of Kashan during four seasons and were necropsied. Different organs including: kidney, heart, liver, lungs, gastrointestinal tract and abdominal cavity were inspected for helminthes and protozoa infection. Animal's characters including: genus, weight, and season, location, microscopic and macroscopic findings were recorded in a special form. Data were classified and statistically analyzed with a confidence interval of 95%. Chi- Squire Test was used to show the relationship between different factors and parasitic infection. From a total 113 stray cats examined, 67(59.3%) were male and 46(40.7%) were female. Fifteen species of endoparasite including helminthes and protozoa were detected in intestine and fecal sample of the examined cats. There were six protozoa, five cestodes and four nematodes. All endoparasite were localized in the gastrointestinal tract. Overall 108 cats (95.6%) have been infected with at least one of the endoparasites. Prevalences of parasites found were Nematodea: Toxocara cati 13.3%, Physaloptera preputialis 39.8%, Rictularia 52.2% and Uncinaria stenocephala 1.8%; Cestodea: Mesocestoides lineatus 7.1%, Taenia taeniaformis 15%, Diplopylidium nolleri 64.6%, Dipylidium caninum 68.1% and Joyeuxiella echinorhyncoides 85%; Sporozoea: Isospora rivolta 5.3%, Isospora felis 5.3%, Sarcocystis spp 8%, Blastocystis spp 16.8% and Zoomastigophorea: Giardia felis 0.9% and Trichomonas spp 1.8%. Contamination rate for zoonotic parasites of cat was greater than expected in Kashan region. In this respect, appropriate control measures should be taken and it is recommended to determine the most appropriate preventive

  4. Refinement of a commercial bench-top relaxin assay for pregnancy diagnosis using urine from domestic and nondomestic felids.

    Science.gov (United States)

    Harris, Laurie A; Steinetz, Bernard G; Bond, Jennifer B; Lasano, Sally; Swanson, William F

    2008-06-01

    Relaxin, a 6-kDa polypeptide hormone, is excreted in the urine during pregnancy in several mammalian species. A recent study showed that detection of urinary relaxin using a bench-top serum assay (Witness relaxin kit, Synbiotics Corp., San Diego, California 92127, USA) can be diagnostic for pregnancy in domestic cats (Felis silvestris catus), but it is unknown whether the bench-top kit is applicable with urine across felid species. Our objectives were to 1) examine modifications in urine processing to improve kit reliability in pregnant cats, 2) evaluate the impact of concentrating urine via filtration on relaxin detection, 3) assess the effect of sample freezing on relaxin concentrations, and 4) begin quantifying urinary relaxin levels in nondomestic felids. Urine and serum were collected from domestic cats and nondomestic cat species (Pallas' cat, Otocolobus manul; sand cat, Felis margarita; cheetah, Acinonyx jubatus; and lion, Panthera leo) at several times after breeding. Urine samples, subjected to various processing methods, were tested using the bench-top kit, and relaxin levels were later quantified via radioimmunoassay. For domestic cat urine samples, filtration and addition of protein/phosphate buffer improved the consistency of the relaxin kit for early pregnancy diagnosis. Urine freezing caused a slight (approximately 13%) but significant decrease in relaxin concentrations, but frozen-thawed samples still tested positive with the bench-top kit. In nondomestic felids, urinary relaxin immunoreactivity during pregnancy was similar to or higher than that of pregnant domestic cats, suggesting that relaxin is a reliable cross-species marker of pregnancy. Urinary relaxin was detectable using the bench-top kit in pregnant Pallas' cats, but urine samples from other species tested negative, regardless of processing methods. Findings suggest that measurement of urinary relaxin is a promising approach for noninvasive pregnancy diagnosis in exotic felids, but

  5. Anti-Rickettsia spp. antibodies in free-ranging and captive capybaras from southern Brazil

    Directory of Open Access Journals (Sweden)

    Fernanda S. Fortes

    2011-11-01

    Full Text Available Capybaras (Hydrochaeris hydrochaeris are among the main hosts of Amblyomma spp. ticks, which is able to transmit Rickettsia species to human beings and animals. Since they are often infested with potential vector ticks, capybaras may be used as sentinels for rickettsiosis, such as the Brazilian Spotted Fever. The aim of the present study was to determine the prevalence of antibodies against Rickettsia spp. using the indirect immunofluorescence assay (IFA in 21 free-ranging and 10 captive animals from the Zoological Park of the 'Bela Vista Biological Sanctuary' (BVBS, Itaipu Binational, Foz do Iguaçu, Southern Brazil. Antigens of six rickettsial species already identified in Brazil (Rickettsia rickettsii, R. parkeri, R. bellii, R. rhipicephali, R. amblyommii and R. felis were used for IFA. Ticks from each capybara were collected for posterior taxonomic identification. A total of 19 (61.3% samples reacted to at least one of tested species. Seropositivity was found in 14 (45.2%, 12 (38.7%, 5 (16.1%, 4 (12.9%, 3 (9.7% and 3 (9.7% animals for R. rickettsii, R. bellii, R. parkeri, R. amblyommii, R. felis and R. rhipicephali, respectively. Two captive capybaras presented suggestive titers of R. rickettsii infection and one sample showed homologous reaction to R. parkeri. Only one free-ranging capybara presented evidence R. bellii infection. Ticks collected on capybaras were identified as Amblyomma dubitatum e Amblyomma sp. Results evidenced the rickettsial circulation in the area, suggesting a potential role of capybaras on bacterial life cycle.

  6. Prevalence and genetic characteristics of Cryptosporidium, Enterocytozoon bieneusi and Giardia duodenalis in cats and dogs in Heilongjiang province, China.

    Science.gov (United States)

    Li, Wei; Li, Yijing; Song, Mingxin; Lu, Yixin; Yang, Jinping; Tao, Wei; Jiang, Yanxue; Wan, Qiang; Zhang, Siwen; Xiao, Lihua

    2015-03-15

    This study investigated 319 fecal specimens of cats (n=52) and dogs (n=267) from Heilongjiang province, China for the prevalence and genetic characteristics of Cryptosporidium, Enterocytozoon bieneusi, and Giardia duodenalis. PCR and DNA sequence analysis of the small subunit rRNA gene identified C. felis and C. parvum in one cat each (3.8%) and C. canis and C. ubiquitum in 6 dogs (2.2%). Polymorphisms in the ribosomal internal transcribed spacer and phylogenetic analysis characterized zoonotic E. bieneusi genotypes D, EbpC, NED1, and NED2 and host-adapted ones NED3, NED4, and PtEb IX in 18 dogs (6.7%) and human-pathogenic genotypes D and IV in 3 cats (5.8%). Genotyping based on the hypermutation of G. duodenalis triosephosphate isomerase gene (TPI) facilitated identification of assemblage F in a cat (1.9%) and assemblages C and E in 12 dogs (4.5%). Subtypes of G. duodenalis isolates were determined by measuring the diversity of both TPI nucleotide and amino acid sequences. C. canis, C. felis, C. parvum, E. bieneusi genotypes D, EbpC, and IV, and G. duodenalis assemblage C identified herein have been documented in human infections in China. C. canis, C. parvum, C. ubiquitum, and E. bieneusi genotypes D, EbpC, and IV carried by cats or dogs also existed in wastewater in China. The finding suggested pet animals could be reservoirs for human cryptosporidiosis, microsporidiosis, and giardiasis and potential sources of water contamination in China.

  7. Hepatozoon and Theileria species detected in ticks collected from mammals and snakes in Thailand.

    Science.gov (United States)

    Sumrandee, Chalao; Baimai, Visut; Trinachartvanit, Wachareeporn; Ahantarig, Arunee

    2015-04-01

    We report the detection of Hepatozoon and Theileria in 103 ticks from mammals and snakes in Thailand. By using a genus-specific 18S rRNA PCR, Hepatozoon and Theileria spp. were detected in 8% and 18%, respectively, of ticks (n=79) removed from mammals. Of the ticks removed from snakes (n=24), 96% were infected with Hepatozoon spp., but none were infected with Theileria. Phylogenetic analysis revealed that Hepatozoon spp. detected from Dermacentor astrosignatus and Dermacentor auratus ticks from Wild boar (Sus scrofa) formed a phylogenetic group with many isolates of Hepatozoon felis that were distantly related to a species group containing Hepatozoon canis and Hepatozoon americanum. In contrast, a phylogenetic analysis of the Hepatozoon sequences of snake ticks revealed that Hepatozoon spp. from Amblyomma varanense from King cobra (Ophiophagus hannah) and Amblyomma helvolum ticks from Indochinese rat snake (Ptyas korros), and Asiatic water snake (Xenochrophis piscator) are grouped with Hepatozoon spp. recently isolated from Monocellate cobras, Reticulated pythons and Burmese pythons, all of Thai origin, and with Hepatozoon sp. 774c that has been detected from a tick species obtained from Argus monitors in Australia. A phylogenetic analysis demonstrated that Theileria spp. from Rhipicephalus (Boophilus) microplus, Haemaphysalis obesa, and Haemaphysalis lagrangei ticks from Sambar deer (Cervus unicolor) cluster with the Theileria cervi isolates WU11 and 239, and Theileria sp. Iwate 141. We report for the first time a Hepatozoon species that shares genetic similarity with Hepatozoon felis found in Dermacentor astrosignatus and Dermacentor auratus ticks collected from Wild boars in Thailand. In addition, we found the presence of a Theileria cervi-like sp. which suggests the potential role of Haemaphysalis lagrangei as a Theileria vector in Thailand.

  8. Transposon insertion reveals pRM, a plasmid of Rickettsia monacensis.

    Science.gov (United States)

    Baldridge, Gerald D; Burkhardt, Nicole Y; Felsheim, Roderick F; Kurtti, Timothy J; Munderloh, Ulrike G

    2007-08-01

    Until the recent discovery of pRF in Rickettsia felis, the obligate intracellular bacteria of the genus Rickettsia (Rickettsiales: Rickettsiaceae) were thought not to possess plasmids. We describe pRM, a plasmid from Rickettsia monacensis, which was detected by pulsed-field gel electrophoresis and Southern blot analyses of DNA from two independent R. monacensis populations transformed by transposon-mediated insertion of coupled green fluorescent protein and chloramphenicol acetyltransferase marker genes into pRM. Two-dimensional electrophoresis showed that pRM was present in rickettsial cells as circular and linear isomers. The 23,486-nucleotide (31.8% G/C) pRM plasmid was cloned from the transformant populations by chloramphenicol marker rescue of restriction enzyme-digested transformant DNA fragments and PCR using primers derived from sequences of overlapping restriction fragments. The plasmid was sequenced. Based on BLAST searches of the GenBank database, pRM contained 23 predicted genes or pseudogenes and was remarkably similar to the larger pRF plasmid. Two of the 23 genes were unique to pRM and pRF among sequenced rickettsial genomes, and 4 of the genes shared by pRM and pRF were otherwise found only on chromosomes of R. felis or the ancestral group rickettsiae R. bellii and R. canadensis. We obtained pulsed-field gel electrophoresis and Southern blot evidence for a plasmid in R. amblyommii isolate WB-8-2 that contained genes conserved between pRM and pRF. The pRM plasmid may provide a basis for the development of a rickettsial transformation vector.

  9. Genetic relatedness versus biological compatibility between Aspergillus fumigatus and related species.

    Science.gov (United States)

    Sugui, Janyce A; Peterson, Stephen W; Figat, Abigail; Hansen, Bryan; Samson, Robert A; Mellado, Emilia; Cuenca-Estrella, Manuel; Kwon-Chung, Kyung J

    2014-10-01

    Aspergillus section Fumigati contains 12 clinically relevant species. Among these Aspergillus species, A. fumigatus is the most frequent agent of invasive aspergillosis, followed by A. lentulus and A. viridinutans. Genealogical concordance and mating experiments were performed to examine the relationship between phylogenetic distance and mating success in these three heterothallic species. Analyses of 19 isolates from section Fumigati revealed the presence of three previously unrecognized species within the broadly circumscribed species A. viridinutans. A single mating type was found in the new species Aspergillus pseudofelis and Aspergillus pseudoviridinutans, but in Aspergillus parafelis, both mating types were present. Reciprocal interspecific pairings of all species in the study showed that the only successful crosses occurred with the MAT1-2 isolates of both A. parafelis and A. pseudofelis. The MAT1-2 isolate of A. parafelis was fertile when paired with the MAT1-1 isolates of A. fumigatus, A. viridinutans, A. felis, A. pseudoviridinutans, and A. wyomingensis but was not fertile with the MAT1-1 isolate of A. lentulus. The MAT1-2 isolates of A. pseudofelis were fertile when paired with the MAT1-1 isolate of A. felis but not with any of the other species. The general infertility in the interspecies crossings suggests that genetically unrelated species are also biologically incompatible, with the MAT1-2 isolates of A. parafelis and A. pseudofelis being the exception. Our findings underscore the importance of genealogical concordance analysis for species circumscription, as well as for accurate species identification, since misidentification of morphologically similar pathogens with differences in innate drug resistance may be of grave consequences for disease management.

  10. Neoplasia in felids at the Knoxville Zoological Gardens, 1979-2003.

    Science.gov (United States)

    Owston, Michael A; Ramsay, Edward C; Rotstein, David S

    2008-12-01

    A review of medical records and necropsy reports from 1979-2003 found 40 neoplasms in 26 zoo felids, including five lions (Panthera leo, two males and three females), three leopards (Panthera pardus, two males and one female), one jaguar (Panthera onca, female), 11 tigers (Panthera tigris, three males and eight females), two snow leopards (Panthera uncia, one male and one female), two cougars (Felis concolor, one male and one female), one bobcat (Felis rufus, male), and one cheetah (Acinonyx jubatus, female). Animals that had not reached 3 yr of age or had been housed in the collection less than 3 yrs were not included in the study. Neoplasia rate at necropsy was 51% (24/47), and overall incidence of felid neoplasia during the study period was 25% (26/103). Neoplasia was identified as the cause of death or reason for euthanasia in 28% (13/47) of those necropsied. Neoplasms were observed in the integumentary-mammary (n=11), endocrine (n=10), reproductive (n=8), hematopoietic-lymphoreticular (n=5), digestive (n=3), and hepatobiliary (n=2) systems. One neoplasm was unclassified by system. Multiple neoplasms were observed in 11 animals. Both benign and malignant neoplasms were observed in all systems except for the hematopoietic-lymphoreticular systems where all processes were malignant. Of the endocrine neoplasms, those involving the thyroid and parathyroid glands predominated (n=8) over other endocrine organs and included adenomas and carcinomas. In the integumentary system, 63% (7/11) of neoplasms involved the mammary gland, with mammary carcinoma representing 83% (6/7) of the neoplasms. The rates of neoplasia at this institution, during the given time period, appears to be greater than rates found in the one other published survey of captive felids.

  11. Landscape-level Connectivity in Coastal Southern California, USA, as Assessed through Carnivore Habitat Suitability

    Science.gov (United States)

    Hunter, R.D.; Fisher, R.N.; Crooks, K.R.

    2003-01-01

    Although the fragmentation of the natural landscape of coastal southern California, USA, is accelerating, large-scale assessments of regional connectivity are lacking. Because of their large area requirements and long dispersal movements, mammalian carnivores can be effective focal species to use when evaluating landscape-level connectivity. Our goal was to make an initial assessment of the extent of landscape-level connectivity in coastal southern California using mountain lions (Felis concolor [Linnaeusl) and bobcats (Felis rufus [Shreber]) as focal species. We first characterized habitat preferences for mountain lions and bobcats from previously derived habitat relationship models for these species; the resulting maps provided a coarse view of habitat preferences for use at regional scales. We then constructed GIS models to evaluate the disturbance impact of roadways and development, major determinants of carnivore distribution and abundance in the south coast region. Finally, we combined the habitat relationship models with the disturbance impact models to characterize habitat connectivity for mountain lions and bobcats in the ecoregion. Habitat connectivity in the ecoregion appeared higher for bobcats than for mountain lions due in part to higher habitat suitability for bobcats in coastal lowland areas. Our models suggest that much of the key carnivore habitat in the coastal southern California is at risk; over 80% of high suitability habitat and over 90% of medium suitability habitat for carnivores is found in the least protected land management classes. Overall, these models allow for (1) identification of core habitat blocks for carnivores and key landscape connections between core areas, (2) evaluation of the level of protection of these areas, and (3) a regional framework within which to develop and coordinate local management and conservation plans.

  12. A Study on Faunal Remains from the Yuxi Site%玉溪遗址动物骨骼初步研究

    Institute of Scientific and Technical Information of China (English)

    赵静芳; 袁东山

    2012-01-01

    Located in Three Gorges Area, this site is dated from middle and late Neolithic to Shangand Zhou period. The Number of Identified Specimens is 7755 at the site, which represents 28 species. They are Cervus unicolor, Muntiacus reeves, Bubalus bubalus, Sus domestica, Selenaretos thibetanus, Aretonyx eollaris, Canis familiaris, Felis bengalensis, Felis silvestris, Panthera tigris, Paguma larvata, Rhinoceros sp., Hystrix hodgsoni, Rhizomys sp., Macaca mulatta, Mylopharyngodon piceus, Cteno- pharyngodon ideUus, Silurus asotus, Acipenser sp., Chinemys reevesi, Trionychidae indet, AVES,and MOLLUSCA. Among these species, pigs and dogs are domesticated, while others are all wild animals. According to the statistic result of modification marks on animal bones, it is speculated that the process of dismembering animals and the procedure of manufacturing awl-shaped bone tools occurred at the site. The wild species suggest that the climate of the site might be subtropical at that time.%三峡地区的玉溪遗址跨越了新石器时代中晚期和商周时期,出土了丰富的动物骨骼。动物群包括28个种,其中猪、狗为家养动物,其余为野生动物。通过对动物骨骼上人工痕迹的观察统计,复原了肢解动物的程序以及“锥形器”的制作流程。野生动物则指示了遗址周围为亚热带气候。

  13. Molecular detection of bacterial and parasitic pathogens in hard ticks from Portugal.

    Science.gov (United States)

    Maia, Carla; Ferreira, Andreia; Nunes, Mónica; Vieira, Maria Luísa; Campino, Lenea; Cardoso, Luís

    2014-06-01

    Ticks are important vector arthropods of human and animal pathogens. As information about agents of disease circulating in vectors in Portugal is limited, the aim of the present study was to detect bacteria and parasites with veterinary and zoonotic importance in ticks collected from dogs, cats, and field vegetation. A total of 925 ticks, comprising 888 (96.0%) adults, 8 (0.9%) nymphs, and 29 (3.1%) larvae, were collected in 4 geographic areas (districts) of Portugal. Among those, 620 (67.0%) were removed from naturally infested dogs, 42 (4.5%) from cats, and 263 (28.4%) were questing ticks obtained from field vegetation. Rhipicephalus sanguineus was the predominant tick species, and the only one collected from dogs and vegetation, while all Ixodes ricinus specimens (n=6) were recovered from cats. Rickettsia massiliae and Rickettsia conorii were identified in 35 ticks collected from cats and dogs and in 3 ticks collected from dogs. Among ticks collected from cats or dogs, 4 Rh. sanguineus specimens were detected with Hepatozoon felis, 3 with Anaplasma platys, 2 with Hepatozoon canis, one with Anaplasma phagocytophilum, one with Babesia vogeli, one with Borrelia burgdorferi sensu lato and one with Cercopithifilaria spp. Rickettsia helvetica was detected in one I. ricinus tick collected from a cat. To the best of our knowledge, this was the first time that Cercopithifilaria spp., Ba. vogeli, H. canis, and H. felis have been detected in ticks from Portugal. The wide range of tick-borne pathogens identified, some of zoonotic concern, suggests a risk for the emergence of tick-borne diseases in domestic animals and humans in Portugal. Further studies on these and other tick-borne agents should be performed to better understand their epidemiological and clinical importance, and to support the implementation of effective control measures.

  14. Molecular detection of feline arthropod-borne pathogens in cats in Cuiabá, state of Mato Grosso, central-western region of Brazil

    Directory of Open Access Journals (Sweden)

    Natasha Gandolfi Miceli

    Full Text Available Hemotrophic mycoplasmas (hemoplasmas, Bartonellasp., Hepatozoon sp. and Cytauxzoon felis are prominent pathogens that circulate between cats and invertebrate hosts. The present study aimed to detect the presence of DNA from hemoplasmas,Bartonella sp., Hepatozoon sp. andCytauxzoon felis, and then confirm it by means of sequencing, in blood samples from cats in Cuiabá, MT, Brazil. From February 2009 to February 2011, blood samples with added EDTA were collected from 163 cats that were being housed in four different animal shelters in the city of Cuiabá, state of Mato Grosso, Brazil and from 15 cats that were admitted to the veterinary hospital of the Federal University of Mato Grosso (UFMT. Out of the 178 cats sampled, 15 (8.4% were positive for hemoplasmas: four (2.2% forMycoplasma haemofelis, 12 (6.7% for ‘Candidatus M. haemominutum’ and one (0.5% for ‘Candidatus M. turicensis’. One cat (0.5%, a patient that was attended at the veterinary hospital, was coinfected with M. haemofelis, ‘Candidatus M. haemominutum’ and ‘Candidatus M. turicensis’, based on sequencing confirmation. Four cats were positive for Bartonella spp.: three (1.7% for B. henselae and one (0.5% for B. clarridgeiae. None of the animals showedCytauxzoon sp. or Hepatozoon sp. DNA in their blood samples. This study showed that cats housed in animal shelters in the city of Cuiabá, state of Mato Grosso, are exposed to hemoplasmas andBartonella species.

  15. ECTOFAUNA PARASITÁRIA DE CÃES URBANOS DOMICILIADOS ATENDIDOS EM CLÍNICAS VETERINÁRIAS PARTICULARES NA CIDADE DE LAVRAS, MG

    Directory of Open Access Journals (Sweden)

    Antonio Marcos Guimarães

    2011-03-01

    Full Text Available The objective of this study was to determine the frequency of ectoparasites in urban domiciled dogs treated at nine private veterinary clinics in the city of Lavras, Minas Gerais, Brazil. Visible and palpable ectoparasites specimens were collected manually from 67 dogs during the period of September 2004 to May 2005, kept in flasks with ethanol 70º GL and identified under a stereomicroscope. In the case of mites that cause mange, performed the analysis of the results obtained from 155 skin scrapings of the same number of dogs with clinical suspicion of parasitic dermatosis was performed, and sent to diagnostic at the Laboratory of Parasitic Diseases of Federal University of Lavras (UFLA, during the period of September 2002 to July 2007. A total of 540 ectoparasites were recorded, and four species were identified: Rhipicephalus sanguineus (60%, Ctenocephalides canis and C. felis (both 36%, and Dermatobia hominis larvae (4%. In scraped skin, positive results were verified in 12.9% (20/155, of which 80.0% with Demodex canis (16/20 and 20.0% with Sarcoptes scabiei var. canis (4/20. After ectoparasites identification a database with the dogs´medical records was made and analyzed by SPSS 12.0. It can be concluded that the predominant species of ectoparasites in dogs treated at private veterinary clinics in the city of Lavras, MG, were Ctenocephalides canis and C. felis (Siphonaptera: Pulicidae and R. sanguineus (Acari: Ixodidae and Demodex canis (Acari: Demodecidae that had a significant predominance (p<0.05 on scraped skin of young animals with suspicion of canine demodicosis.

  16. Predictors for abundance of host flea and floor flea in households of villages with endemic commensal rodent plague, Yunnan Province, China.

    Directory of Open Access Journals (Sweden)

    Jia-Xiang Yin

    Full Text Available BACKGROUND: From 1990 to 2006, fifty-five natural villages experienced at least one plague epidemic in Lianghe County, Yunnan Province, China. This study is aimed to document flea abundance and identify predictors in households of villages with endemic commensal rodent plague in Lianghe County. METHODS: Trappings were used to collect fleas and interviews were conducted to gather demography, environmental factors, and other relevant information. Multivariate hurdle negative binomial model was applied to identify predictors for flea abundance. RESULTS: A total of 344 fleas were collected on 101 small mammals (94 Rattus flavipectus and 7 Suncus murinus. R. flavipectus had higher flea prevalence and abundance than S. murinus, but the flea intensities did not differ significantly. A total of 315 floor fleas were captured in 104 households. Xenopsylla cheopis and Ctenocephalides felis felis were the predominant flea species on the host and the floor flea, respectively. The presence of small mammal faeces and R. flavipectus increased host flea prevalence odds 2.9- and 10-fold, respectively. Keeping a dog in the house increased floor flea prevalence odds 2-fold. Keeping cattle increased floor flea intensity by 153%. Villages with over 80% of houses raising chickens had increased prevalence odds and intensity of floor flea about 2.9- and 11.6-fold, respectively. The prevalence and intensity of floor flea in brick and wood houses were decreased by 60% and 90%, respectively. Flea prevalences of host and floor flea in the households that were adjacent to other houses were increased 7.4- and 2.2-fold, respectively. Houses with a paddy nearby decreased host flea intensity by 53%, while houses with an outside toilet increased host flea intensity by 125%. CONCLUSION: Rodent control alone may not be sufficient to control plague risk in these areas. In order to have successful results, plague control programs should pay attention to ecological and hygiene factors

  17. What makes a feline fatal in Toxoplasma gondii's fatal feline attraction? Infected rats choose wild cats.

    Science.gov (United States)

    Kaushik, M; Knowles, S C L; Webster, J P

    2014-07-01

    Toxoplasma gondii is an indirectly transmitted protozoan parasite, of which members of the cat family (Felidae) are the only definitive hosts and small mammals such as rats serve as intermediate hosts. The innate aversion of rodents to cat odor provides an obstacle for the parasite against successful predation by the feline definitive host. Previous research has demonstrated that T. gondii appears to alter a rat's perception of the risk of being preyed upon by cats. Although uninfected rats display normal aversion to cat odor, infected rats show no avoidance and in some cases even show attraction to cat odor, which we originally termed the "Fatal Feline Attraction." In this study, we tested for the first time whether the "Fatal Feline Attraction" of T. gondii-infected rats differed according to the type of feline odor used, specifically whether it came from domestic cats (Felis silvestris catus) or wild cats-cheetahs (Acinonyx jubatus) or pumas (Felis concolor). In two-choice odor trials, where wild and domestic cat odors were competed against one another, consistent with previous findings we demonstrated that infected rats spent more time in feline odor zones compared with uninfected rats. However, we further demonstrated that all cat odors are not equal: infected rats had a stronger preference for wild cat odor over that of domestic cats, an effect that did not differ significantly according to the type of wild cat odor used (cheetah or puma). We discuss these results in terms of the potential mechanism of action and their implications for the current and evolutionary role of wild, in addition to domestic, cats in transmission of T. gondii.

  18. Molecular detection of Rickettsia typhi in cats and fleas.

    Directory of Open Access Journals (Sweden)

    Maria Mercedes Nogueras

    Full Text Available BACKGROUND: Rickettsiatyphi is the etiological agent of murine typhus (MT, a disease transmitted by two cycles: rat-flea-rat, and peridomestic cycle. Murine typhus is often misdiagnosed and underreported. A correct diagnosis is important because MT can cause severe illness and death. Our previous seroprevalence results pointed to presence of human R. typhi infection in our region; however, no clinical case has been reported. Although cats have been related to MT, no naturally infected cat has been described. The aim of the study is to confirm the existence of R. typhi in our location analyzing its presence in cats and fleas. METHODOLOGY/PRINCIPAL FINDINGS: 221 cats and 80 fleas were collected from Veterinary clinics, shelters, and the street (2001-2009. Variables surveyed were: date of collection, age, sex, municipality, living place, outdoor activities, demographic area, healthy status, contact with animals, and ectoparasite infestation. IgG against R. typhi were evaluated by indirect immunofluorescence assay. Molecular detection in cats and fleas was performed by real-time PCR. Cultures were performed in those cats with positive molecular detection. Statistical analysis was carried out using SPSS. A p < 0.05 was considered significant. Thirty-five (15.8% cats were seropositive. There were no significant associations among seropositivity and any variables. R. typhi was detected in 5 blood and 2 cultures. High titres and molecular detection were observed in stray cats and pets, as well as in spring and winter. All fleas were Ctenocephalides felis. R. typhi was detected in 44 fleas (55%, from shelters and pets. Co-infection with R. felis was observed. CONCLUSIONS: Although no clinical case has been described in this area, the presence of R. typhi in cats and fleas is demonstrated. Moreover, a considerable percentage of those animals lived in households. To our knowledge, this is the first time R. typhi is detected in naturally infected cats.

  19. Gastric Helicobacter infection induces iron deficiency in the INS-GAS mouse.

    Directory of Open Access Journals (Sweden)

    Melanie J Thomson

    Full Text Available There is increasing evidence from clinical and population studies for a role of H. pylori infection in the aetiology of iron deficiency. Rodent models of Helicobacter infection are helpful for investigating any causal links and mechanisms of iron deficiency in the host. The aim of this study was to investigate the effects of gastric Helicobacter infection on iron deficiency and host iron metabolism/transport gene expression in hypergastrinemic INS-GAS mice. INS-GAS mice were infected with Helicobacter felis for 3, 6 and 9 months. At post mortem, blood was taken for assessment of iron status and gastric mucosa for pathology, immunohistology and analysis of gene expression. Chronic Helicobacter infection of INS- GAS mice resulted in decreased serum iron, transferrin saturation and hypoferritinemia and increased Total iron binding capacity (TIBC. Decreased serum iron concentrations were associated with a concomitant reduction in the number of parietal cells, strengthening the association between hypochlorhydria and gastric Helicobacter-induced iron deficiency. Infection with H. felis for nine months was associated with decreased gastric expression of iron metabolism regulators hepcidin, Bmp4 and Bmp6 but increased expression of Ferroportin 1, the iron efflux protein, iron absorption genes such as Divalent metal transporter 1, Transferrin receptor 1 and also Lcn2 a siderophore-binding protein. The INS-GAS mouse is therefore a useful model for studying Helicobacter-induced iron deficiency. Furthermore, the marked changes in expression of gastric iron transporters following Helicobacter infection may be relevant to the more rapid development of carcinogenesis in the Helicobacter infected INS-GAS model.

  20. Gastric Helicobacter infection induces iron deficiency in the INS-GAS mouse.

    Science.gov (United States)

    Thomson, Melanie J; Pritchard, D Mark; Boxall, Sally A; Abuderman, Abdul A; Williams, Jonathan M; Varro, Andrea; Crabtree, Jean E

    2012-01-01

    There is increasing evidence from clinical and population studies for a role of H. pylori infection in the aetiology of iron deficiency. Rodent models of Helicobacter infection are helpful for investigating any causal links and mechanisms of iron deficiency in the host. The aim of this study was to investigate the effects of gastric Helicobacter infection on iron deficiency and host iron metabolism/transport gene expression in hypergastrinemic INS-GAS mice. INS-GAS mice were infected with Helicobacter felis for 3, 6 and 9 months. At post mortem, blood was taken for assessment of iron status and gastric mucosa for pathology, immunohistology and analysis of gene expression. Chronic Helicobacter infection of INS- GAS mice resulted in decreased serum iron, transferrin saturation and hypoferritinemia and increased Total iron binding capacity (TIBC). Decreased serum iron concentrations were associated with a concomitant reduction in the number of parietal cells, strengthening the association between hypochlorhydria and gastric Helicobacter-induced iron deficiency. Infection with H. felis for nine months was associated with decreased gastric expression of iron metabolism regulators hepcidin, Bmp4 and Bmp6 but increased expression of Ferroportin 1, the iron efflux protein, iron absorption genes such as Divalent metal transporter 1, Transferrin receptor 1 and also Lcn2 a siderophore-binding protein. The INS-GAS mouse is therefore a useful model for studying Helicobacter-induced iron deficiency. Furthermore, the marked changes in expression of gastric iron transporters following Helicobacter infection may be relevant to the more rapid development of carcinogenesis in the Helicobacter infected INS-GAS model.

  1. Tiger, Bengal and Domestic Cat Embryos Produced by Homospecific and Interspecific Zona-Free Nuclear Transfer.

    Science.gov (United States)

    Moro, L N; Jarazo, J; Buemo, C; Hiriart, M I; Sestelo, A; Salamone, D F

    2015-10-01

    The aim of this study was to evaluate three different cloning strategies in the domestic cat (Felis silvestris) and to use the most efficient to generate wild felid embryos by interspecific cloning (iSCNT) using Bengal (a hybrid formed by the cross of Felis silvestris and Prionailurus bengalensis) and tiger (Panthera tigris) donor cells. In experiment 1, zona-free (ZP-free) cloning resulted in higher fusion and expanded blastocyst rates with respect to zona included cloning techniques that involved fusion or injection of the donor cell. In experiment 2, ZP-free iSCNT and embryo aggregation (2X) were assessed. Division velocity and blastocyst rates were increased by embryo aggregation in the three species. Despite fewer tiger embryos than Bengal and cat embryos reached the blastocyst stage, Tiger 2X group increased the percentage of blastocysts with respect to Tiger 1X group (3.2% vs 12.1%, respectively). Moreover, blastocyst cell number was almost duplicated in aggregated embryos with respect to non-aggregated ones within Bengal and tiger groups (278.3 ± 61.9 vs 516.8 ± 103.6 for Bengal 1X and Bengal 2X groups, respectively; 41 vs 220 ± 60 for Tiger 1X and Tiger 2X groups, respectively). OCT4 analysis also revealed that tiger blastocysts had higher proportion of OCT4-positive cells with respect to Bengal blastocysts and cat intracytoplasmic sperm injection blastocysts. In conclusion, ZP-free cloning has improved the quality of cat embryos with respect to the other cloning techniques evaluated and was successfully applied in iSCNT complemented with embryo aggregation.

  2. Tissue-specific mercury concentrations in two catfish species from the Brazilian coast

    Directory of Open Access Journals (Sweden)

    Juliana de Souza Azevedo

    2012-06-01

    Full Text Available Some regions of Brazil have particularly high levels of mercury (Hg emissions due to industrial activities, and their fish species may therefore suffer high levels of contamination through bioaccumulation. In this paper we assess Hg contamination in the muscle, liver, gills and blood of two different species of catfish collected from the Cananéia and Santos-São Vicente estuaries, São Paulo State, Brazil. Fish from the most polluted site (Santos-São Vicente showed higher median Hg concentrations in both species (Cathorops spixii - liver: 1530 µg Kg-1, muscle: 327 µg Kg-1 and gill: 101 µg Kg-1; Genidens genidens - liver: 2617 µg Kg-1, muscle: 393 µg Kg-1 and gill: 118 µg Kg-1. Multivariate analysis revealed the importance and influence of key biological variables (size, condition, etc. in determining the overall level of Hg and its distribution within different tissues.Algumas regiões do Brasil apresentam, altos níveis de emissões de mercúrio (Hg devido às atividades industriais, e suas espécies de peixes podem, portanto, estar expostos a elevados níveis de contaminação por meio da bioacumulação. Neste trabalho avaliamos a contaminação por Hg no músculo, fígado, brânquias e sangue de duas espécies de bagres coletados nos estuários de Cananéia e Santos-São Vicente, São Paulo, Brasil. Peixes amostrados no local mais poluído (Santos-São Vicente mostraram maiores concentrações de Hg em ambas as espécies (Cathorops spixii - fígado: 1530 µg Kg-1, músculo: 327 µg Kg-1 e brânquias: 101 µg Kg-1; Genidens genidens - fígado: 2617 µg Kg-1, músculo: 393 µg Kg-1 e brânquias: 118 µg Kg-1. Análises multivariadas revelaram a importância e influência das principais variáveis biológicas (tamanho, condição, etc. no teor geral de Hg e sua distribuição nos diferentes tecidos.

  3. Sciades herzbergii oxidative stress biomarkers: an in situ study of an estuarine ecosystem (São Marcos' Bay, Maranhão, Brazil

    Directory of Open Access Journals (Sweden)

    Raimunda Nonata Fortes Carvalho-Neta

    2010-01-01

    Full Text Available In order to study the effects of environmental contamination on wild fish, sites were sampled in São Marcos' Bay. The first is located near the ALUMAR/ALCOA port, a potentially contaminated area. The second, located near the Coqueiro beach, was used as a reference area. The activity of antioxidant defence catalase (CAT and glutathione S-transferase (GST in S. herzbergii was compared with the biometric data and gonadosomatic index (GSI. The result showed that GSI decreased significantly in females (pO objetivo desse trabalho foi estudar os efeitos da contaminação ambiental em peixes amostrados em dois locais da Baía de São Marcos. O primeiro ponto está localizado próximo ao porto da ALUMAR/ALCOA, considerado como uma área potencialmente contaminada. O segundo ponto, situado na praia do Coqueiro, foi usado como uma área de referência. Dados da atividade da enzima de defesa antioxidante catalase (CAT e da glutationa S-transferase (GST em S. herzbergii foram comparados com os dados biométricos e o índice gonadossomático (GSI. Resultados mostraram que o GSI diminuiu significativamente em fêmeas (p <0.05 no local contaminado. A atividade da CAT foi mais alta nos peixes do local contaminado. Uma diferença significativa foi observada na atividade de GST de S. herzbergii no local contaminado e no local de referência (p <0.05. GSI possibilitou uma nova abordagem quanto à natureza da resposta de destoxificação nessa espécie de bagre porque este índice não apresentou correlação com as enzimas no local potencialmente contaminado, mas apresentou no local de referência. Assim, sugere-se que a boa correlação da GST/CAT e GSI poderia estar relacionada à reprodução dos animais no local de referência, mas não no local potencialmente contaminado. Se esse for o caso, pode-se concluir que GST/CAT e GSI podem ser utilizados como bons biomarcadores para avaliar contaminação aquática.

  4. Arius kesslerl & Sciadeops troschelii (Pisces: Ariidae growth in floating net cages in estuarine waters of Buenventura Bay-Colombia Crecimiento de Arius kekkleri y Sciadeops troschelli (Pises: Ariidae en jaulas flotantes en zonas estuarinas de la bahía de Buenaventura

    Directory of Open Access Journals (Sweden)

    Arroyo Rafael

    1999-11-01

    Full Text Available

    Two species of estuary catfish the Arius kessleri (CoCoCo and the Sciadeops troschelii (Nato were held in floating net cages at varying densities in estuarine waters of Buenventura Bay-Colombia. After 120 days for the Cococo had weights of 84.1 g Y64 g with densities of 5 and 30 specimen/rn'. The growth average was 0.58-0.39 g/day; the net yield obtained vary from 0.35 to 1.16 Kg/m', the food conversion ratio vary from 3.3 to 5.0 and de survival rate vary from 86% to 100%. With the Nato we obtained weights of 164 and 184 g, beginning with weights of 41 and 108 g their growth average vary from 0.50 to 0.82 g/day. The net yield obtained vary from 0.58 to 0.75 Kg/m' and the survival rate vary from 83% to 100%. From these results we conclude that the two species of catfish studied are strong species but they do not offer good possibilities for fish farming in estuarine waters.

    Dos especies de Bagres estuarinos Arius kessleri (CoCoCo y Sciadeops troschelii (Nato fueron criados en jaulas flotantes a densidades variables en zonas estuarinas de la bahía de Buenaventura. Después de 120 días, para el Cococo se obtuvieron pesos de 84.1 y 64 g a densidades de 5-30 individuos/rrr' con tasas de crecimiento de 0.58 a 0.39 g/día; las producciones netas obtenidas fluctúan entre 0.35-1.16 Kg/m', la supervivencia fluctúa entre 86-100% y las tasas de conversión de alimento f1uctuaron entre 3.3 y 5.0. El Nato alcanza pesos de 164 y 184 g a partir de pesos iniciales de 41 y 108 g; sus tasas de crecimiento f1uctuaron entre 0.50 y 0.82 g/día; las producciones netas obtenidas f1uctuaron entre 0.58 y 0.75 Kg/m', y sus supervivencias fluctuaron entre 83 y 100%. Se concluye que a pesar de su gran rusticidad y supervivencia, estas dos especies ofrecen poco potencial para ser criadas en jaulas en zonas estuarinas.

  5. Phylogeography of the Neotropical catfish Pimelodus albicans (Siluriformes: Pimelodidae from río de la Plata basin, South America, and conservation remarks

    Directory of Open Access Journals (Sweden)

    Julia Vergara

    Full Text Available Pimelodus albicans Valenciennes, 1840 (common name "moncholo" or "bagre blanco" is an endemic species of the family Pimelodidae in the río de la Plata basin. Phylogenetic approach based on cytochrome b sequences was performed to test the existence of a unique evolutionary lineage in P. albicans and to discriminate populations units or subpopulations related to a migration behavior of this taxon in the río de la Plata basin. This study included 34 samples of P. albicans of different collecting sites in the río de la Plata estuary and in the río Arrecifes belonging to the río Paraná basin. Among 614 base pairs in the cytochrome b sequence data set, 203 were variable and 120 were phylogenetically informative sites in P. albicans. A total of twenty haplotypes, nucleotide diversity (p = 0.032 and haplotype diversity = 0.941 were found. Tajima's test showed significant value D= -1.88 (p<0.05 rejecting the neutral mutation hypothesis for the P. albicans data set. All phylogenetic approaches showed that P. albicans included four monophyletic assemblages that were supported by high bootstrap and Bayesian posterior probability values. Minimum spanning network corroborated these groups for P. albicans haplotypes. High genetic structure was found in P. albicans by means of AMOVA analysis showing that the río Arrecifes samples constitute an isolated lineage. Moreover, the high value of genetic divergence (10% between the río de la Plata and the río Arrecifes populations could suggest that P. albicans may be conformed by a sibling species complex. On the other hand, a degree of genetic structuring was detected among different sites of the río de la Plata. A partial isolation of the 760 site may suggest that P. albicans could migrates to different tributaries for reproduction, generating different schools of haplotypes which could mix in the río de la Plata estuary. The high nucleotide diversity found in the 765 site and the existence of gene flow

  6. A preliminary inventory of the catfishes of the lower Rio Nhamundá, Brazil (Ostariophysi, Siluriformes).

    Science.gov (United States)

    Collins, Rupert A; Duarte Ribeiro, Emanuell; Nogueira Machado, Valéria; Hrbek, Tomas; Farias, Izeni Pires

    2015-01-01

    The Rio Nhamundá is a poorly-known clearwater river draining the southern Guiana Shield of Brazil. In this study we report the findings of a preliminary ichthyological survey, focusing on catfishes (Siluriformes). We identify a total of 36 species (31 genera, seven families) from the Nhamundá, including 11 species already recorded from the river. Overall, our survey results show that even rapid surveys can provide important information on Amazon fish biodiversity, suggesting potential new species, providing range extensions for nominal species, and additionally highlighting taxa in need of taxonomic revision and genetic study. As well as the traditional forms of data collected on biodiversity surveys (i.e. preserved specimen vouchers), our study also provides "new" types of data in the form of DNA barcodes and images of fishes exhibiting colouration in life, information that will be invaluable in future work addressing difficult groups. O Rio Nhamundá é um rio de água clara, pouco conhecido, que drena parte do Escudo das Guianas em território brasileiro. Nesse estudo, nós reportamos os resultados de um levantamento ictiofaunístico preliminar dessa área, tendo como foco os bagres (Siluriformes). Nós identificamos um total de 36 espécies (31 gêneros, sete famílias) provenientes de nossa coleta, e adicionamos 11 espécies já conhecidas para o rio. De maneira geral, os resultados de nossa pesquisa mostram que mesmo levantamentos rápidos podem gerar informações importantes sobre a biodiversidade de peixes amazônicos, sugerindo potenciais espécies novas, ampliando a área de distribuição de espécies, além de apontar a necessidade de revisões taxonômicas e estudos genéticos para alguns taxa. Para além das formas tradicionais de dados coletados em pesquisas de biodiversidade (i.e. espécimes preservados), nosso estudo fornece "novas" formas de dados, como DNA barcodes e imagens com o padrão de coloração dos espécimes vivos, informa

  7. Flujos de energía en el sistema de surgencia tropical de la península de la Guajira , Caribe colombiano

    Directory of Open Access Journals (Sweden)

    García Camilo Bernardo

    2004-12-01

    Full Text Available La Guajira es un sistema de surgencia explotado en el Caribe colombiano. Con el propósito de integrar la información disponible sobre el sistema se construyó un modelo trófico de 27 grupos funcionales usando el programa ECOPATH 5.0 Beta. Se obtuvo una base cuantitativa a través de la construcción del modelo, para compararlo con otros sistemas de surgencia. La Guajira presenta una biomasa total de 68 t/km2/año, la producción primaria neta del sistema es de 1248,51 t/km2/año y las transferencias totales del sistema son de 3.275 t/km2/año, lo que
    indica un ecosistema con una surgencia moderada comparada con otros sitios. Las mayores transferencias de energía se dan del nivel trófico I-II (68,93%, aunque existe una importante proporción de flujos al detritus (32%. Las flotas pesqueras afectan principalmente los recursos
    de las fracciones del nivel trófico III, la flota artesanal esta ejerciendo mayor presión sobre el pámpano, bonito y jurel, la flota parguera sobre pargos y meros y la flota industrial sobre las corvinas. Los resultados en los atributos de madurez del sistema sugieren que La Guajira es un
    ecosistema inmaduro debido a que la relación P/R excede a uno y está en desarrollo debido a la baja ascendencia (33,7% y alta capacidad de desarrollo (66,3% como ocurre con otras surgencias donde se presentan valores de ascendencia entre 20% y 35%. Aunque los datos básicos de entrada en el modelo fueron buenos, debido a las investigaciones que se han elaborado entre 1995 y 2000, existen algunos grupos donde no se encuentra aun información disponible. Especialmente en los valores de biomasa del fitoplancton, invertebrados, bagres, peces depredadores
    pelágicos, así como estimaciones de producción en invertebrados, peces  depredadores pelágicos y peces pequeños pelágicos. No existe una cuantificación de las poblaciones de mamíferos y aves en el área de estudio, los cuales constituyen depredadores

  8. La malaria en Colombia, Antioquia y las zonas de Urabá y Bajo Cauca: panorama para interpretar la falla terapéutica antimalárica. Parte 1

    Directory of Open Access Journals (Sweden)

    Jaime Cardona Fonseca

    2003-03-01

    Full Text Available Antecedentes y Problema: en 1998-2002 se investigó en Antioquia (Turbo, Zaragoza y El Bagre, la eficacia terapéutica de medicamentos y combinaciones antimaláricos. La interpretación de la respuesta terapéutica (adecuada, fallas precoz y tardía requiere información adicional para una mejor comprensión. Objetivo: definir un marco de referencia para interpretar los resultados de las evaluaciones de respuesta terapéutica antimalárica, constituido por información sobre el comportamiento de los programas antimaláricos en Colombia y por información demográfica, epidemiológica, climatológica y socioeconómica sobre el departamento de Antioquia, las regiones de Urabá y Bajo Cauca y los municipios de Turbo y El Bagre. Metodología: se usó información oficial secundaria y en menor cantidad, información primaria. Con los datos recogidos se construyeron cuadros y gráficos, y se hicieron cálculos sobre otras variables. Resultados: se describe la malaria (paludismo en Colombia en 1960-2002. En 2002, de los 43,707 millones de habitantes, 22,403 millones (51,17% están expuestos (41,88% con bajo riesgo. El P. vivax es la especie predominante (66% de los casos, excepto en la región del Pacífico, donde el P. falciparum causa el 75% de los casos. Hay brotes de malaria cada 2 a 7 años; la más intensa epidemia en la historia conocida ocurrió en 1998. La mortalidad ha pasado de 25 por cien mil en 1983 a 3 por cien mil en 2000. El índice parasitario anual (IPA ha estado alrededor de 8 por mil expuestos después de 1990, valor que es el cuádruple del existente en 1960-1970. La ineficacia del programa ha sido evidente casi desde su inicio en 1958 y la tendencia de la enfermedad siempre ha sido creciente. El fracaso de los programas gubernamentales antimalaria no tiene origen en fenómenos principalmente biológicos (resistencias del plasmodio a los medicamentos y del anofeles a los insecticidas, ni tampoco en las “condiciones de orden p

  9. Live capture and handling of the European wildcat in central Italy

    Directory of Open Access Journals (Sweden)

    Lolita Bizzarri

    2010-08-01

    Full Text Available Abstract
    Between 2003 and 2006, a live-trapping of European wildcats (Felis silvestris was carried out in the Apennines (central Italy. Double-door tunnel cage traps were set along trap-lines. A box containing live quails as bait was securely attached to the side of each cage. Trapping was carried out in 8 sessions at a total of 60 trap-sites, mainly inside woods (65%. The distance between the traps ranged from 146 m to 907 m and the length of each trap-line ranged from 541 m to 2632 m. There were 16 captures of 11 different wildcats, the capture success rate being 1 wildcat/209 trap-days. Nine males and 2 females were caught, suggesting sex-biased trapping selection. In addition to wildcats, 20 non-target species were captured during the 8 sessions. No animal was injured by the traps and no wildcat was endangered by narcosis or handling. The technique proved to be effective for future field studies that envisage the radio-tracking of wildcats.

    Riassunto
    Cattura e immobilizzazione del gatto selvatico in Italia centrale.
    Tra il 2003 e il 2006 è stato svolto un programma di ricerca sul gatto selvatico europeo (Felis silvestris in un'area dell'Appennino centrale. Trappole a tunnel a doppio ingresso sono state distribuite lungo delle line-trap; a ciascun dispositivo è stato associato un box che ospitava esche vive (quaglie. Sono state effettuate 8 sessioni di trappolamento per un totale di 60 siti trappola, perlopiù in aree boscate (65%. La distanza tra le trappole variava da 146 m a 907 m mentre la lunghezza delle line-trap variava tra 541 m a 2632 m. I 16 eventi di cattura hanno permesso di marcare 11 individui diversi, 9 maschi e 2 femmine. La differenza del numero di catture tra maschi e femmine è probabilmente da attribuire ad una diversa selettività delle trappole. Durante le 8 sessioni, oltre al gatto selvatico, sono state catturate altre

  10. Uso da técnica de Southern Blot/Hibridização associada à reação em cadeia da polimerase para aumentar a sensibilidade no diagnóstico das infecções por hemoplasmas em gatos domésticos: Use of Southern Blot/Hybridization technique associated to polymerase chain reaction to improve the sensitivity in the diagnosis of hemoplasma infections in domestic cats

    Directory of Open Access Journals (Sweden)

    Daniel B. Macieira

    2009-12-01

    Full Text Available O objetivo deste trabalho foi verificar se a técnica de Southern Blot/Hibridização (SB em associação à reação de polimerização em cadeia (PCR aumenta a sensibilidade na detecção de DNA de hemoplasmas em gatos domésticos (Felis catus. O sangue total foi coletado em tubos contendo o anticoagulante ácido etilenodiamino tetra-acético, o DNA extraído a partir de 149 animais e a PCR realizada com o uso de sequências iniciadoras espécie-específicas, para amplificar subunidade 16S do RNA ribossomal de Mycoplasma haemofelis e 'Candidatus M. haemominutum' dessas amostras. Para a hibridização, foram utilizadas sondas específicas quimicamente marcadas, e os resultados visualizados por meio da adição de substrato quimiluminescente seguida de autoradiografia. Dezoito (12,1% das 149 amostras testadas apresentaram resultado PCR-positivo para o DNA de hemoplasmas. A técnica de SB mostrou que 24/149 (16,1% amostras apresentaram resultado positivo para hemoplasmas, confirmando os 18 resultados PCR-positivos, além de revelar seis outros adicionais (p The aim of this study was to determine whether Southern Blot/Hybridization (SB associated to Polymerase Chain Reaction (PCR improves the sensitivity in the detection of hemoplasma DNA in domestic cats (Felis catus. Whole blood was collected in tubes containing the anticoagulant ethylenediamine tetra-acetic acid and DNA extracted from 149 animals. PCR was performed using species specific primers to amplify the 16S ribosomal RNA subunit of Mycoplasma haemofelis and 'Candidatus M. haemominutum' from these samples. Hybridization was performed using a 16S rDNA probes chemically labeled and the results were visualized using a chemiluminescent substrate addition followed by autoradiography. Eighteen (12.1% of the 149 tested samples had a positive PCR result for hemoplasma species DNA. SB/hybridization technique showed that 24/149 (16.1% samples were positive for hemoplasmas, confirming the 18 PCR

  11. Predation on dormice in Italy

    Directory of Open Access Journals (Sweden)

    Dino Scaravelli

    1995-05-01

    Full Text Available Abstract The authors analyse available data on the impact of predators on Dormouse populations in Italy. Dormice are found in the diet of 2 snakes (Vipera berus and V. aspis, 2 diurnal birds of prey (Buteo buteo and Aquila chrysaetos, 6 owls (Tyto alba, Strix aluco, Asio otus, Athene noctua, Bubo bubo and Glaucidium passerinum and 9 mammals (Rattus rattus, Ursus arctos, Canis lupus, Vulpes vulpes, Martes martes, M. foina, Meles meles, Felis silvestris and Sus scrofa in a variable percentage of the prey taken. Only Dryomys nitedula was never encountered as a prey item. The most common prey is Muscardinus avellanarius. There are significative regional differences in predation between bioclimatic areas of the Italian peninsula. The contribution of studies on predation to knowledge of Myoxid distribution is discussed. Riassunto Predazione di Mioxidi in Italia - Sono analizzati i dati pubblicati sull'impatto dei predatori sulle popolazioni di Myoxidae in Italia. Myoxidae sono stati riscontrati nelle diete di 2 serpenti (Vipera berus e V. aspis, 2 rapaci diurni (Buteo buteo e Aquila chrysaetos, 6 notturni (Tyto alba, Strix aluco, Asio otus, Athene noctua, Bubo bubo e Glaucidium passerinum e 9 mammiferi (Rattus rattus, Ursus arctos, Canis lupus, Vulpes vulpes, Martes martes, M foina, Meles meles, Felis silvestris e Sus scrofa in percentuale variabile nella comunità di prede. Solo Dryomys nitedula non è mai stato incontrato come preda. La specie piu comunemente predata risulta Muscardinus avellanarius. Sono discusse le

  12. Efficacy of an imidacloprid/flumethrin collar against fleas, ticks, mites and lice on dogs

    Directory of Open Access Journals (Sweden)

    Stanneck Dorothee

    2012-05-01

    Full Text Available Abstract Background The studies reported here were conducted to ascertain the efficacy of imidacloprid/flumethrin incorporated in a slow-release matrix collar, against infestations of dogs by fleas, ticks, mites and lice. Efficacy was evaluated against the flea Ctenocephalides felis felis, the ticks Rhipicephalus sanguineus, Ixodes ricinus, Ixodes scapularis, Dermacentor reticulatus and Dermacentor variabilis, the mite Sarcoptes scabiei and the biting louse Trichodectes canis. Methods Groups of collar-treated dogs (n = 7–10 were infested with fleas and/or ticks at monthly intervals at least, over a period of up to 8 months. Efficacy against fleas was evaluated 24 to 48 h after treatment and 24 h after each re-infestation. Efficacy against ticks was evaluated at 48 h (acaricidal, 6 h (repellent and 48 h (sustained after infestation. The effect of regular shampooing or immersion in water on the efficacy of the collars was also tested. Efficacy against flea larvae was assessed by incubating blanket samples after dog contact with viable flea eggs. Effectiveness against lice and mites was evaluated after treatment of naturally infested animals. With the exception of the mites, efficacy was calculated by comparison with untreated negative control groups. Results Efficacy against fleas (24 h generally exceeded 95%, and against flea larvae it exceeded 99% for 8 months. Sustained acaricidal (48 h efficacy, covering a period of 8 months was 100% against I. ricinus, starting 2 days after treatment (in vivo, and 100% against I. scapularis (in vitro, above 97% against R. sanguineus, generally above 97% against D. reticulatus and above 90% for D. variabilis. Repellent (6 h efficacy 2 days after treatment and continuing for 8 months was consistently 100% against I. ricinus, and above 90% against R. sanguineus. Regular shampooing affected efficacy against fleas and ticks to a lesser extent than regular immersion in water. The

  13. Parasite meningomyelitis in cats in Uruguay Meningomielites parasitária em gatos no Uruguai

    Directory of Open Access Journals (Sweden)

    Rodolfo Rivero

    2011-09-01

    Full Text Available Two outbreaks of progressive hind limb paresis in cats (Felis catus caused by parasitic meningomyelitis in Uruguay are reported. The case studies occurred in 2008 and 2009 respectively, in the rural areas of Fray Bentos (33º 07' 40.39" S and were characterized by hindquarter paralysis. This paralysis was progressive and had a chronic progression of approximately 12 months until the death or euthanasia of the animals. Clinical symptoms started with ataxia of the hindquarters with lateral side-to-side swaying and culminated in total paralysis. Two animals were sent for necropsy in 2009. The main histopathological findings were severe myelitis in the lumbar spinal cord with perivascular cuffing and white matter necrosis, severe nonsuppurative meningitis with thrombi in subarachnoid blood vessels, and intravascular presence of multiple adult parasites. From the morphological characteristics of the parasites and location in the leptomeninges, the parasite was identified as the nematode Gurltia paralysans.São relatados dois surtos de paralisia progressiva dos membros posteriores em gatos (Felis catus, causada por meningomielite parasitária no Uruguai. Os estudos de casos ocorreram entre os anos 2008 e 2009, respectivamente, nas zonas rurais de Fray Bentos (33º 07' 40,39" S e foram caracterizados por paralisia dos membros posteriores. Esta paralisia era progressiva e tinha evolução crônica de aproximadamente 12 meses, até que os animais vinham a óbito ou eram eutanasiados. Os sintomas clínicos começaram com ataxia dos membros posteriores, com movimentos laterais, terminado em paralisia total. Em 2009, dois animais foram encaminhados para necropsia. Os achados histopatológicos foram caracterizados por severa mielite na medula espinhal lombar com manguitos perivasculares linfocitarios e necrose da substância branca, severa meningite não supurativa com trombos nos vasos sanguíneos subaracnóideos, e presença intravascular de m

  14. Effect of interactions with humans on behaviour, mucosal immunity and upper respiratory disease of shelter cats rated as contented on arrival.

    Science.gov (United States)

    Gourkow, Nadine; Phillips, Clive J C

    2015-10-01

    Sustained positive affect may decrease vulnerability to upper respiratory infections in cats admitted to a shelter. Incidence of upper respiratory infections was examined in cats rated as Content upon admission to an animal shelter when provided with or without treatment to sustain contentment. Ninety-six cats rated as Content upon admission were provided with either human interaction, including petting, playing, and grooming, in four 10min sessions/d for 10 days or were exposed to a control treatment of a human standing in front of the cage with eyes averted for the same period. Changes in emotional state and mucosal immune responses were measured daily in treated and control groups. Infectious status was determined upon admission and on days 4 and 10 using combined conjunctival and oropharyngeal swab specimens tested by quantitative real-time PCR for feline herpes virus type 1, feline calicivirus, Mycoplasma felis, Chlamydophila felis, and Bordetella bronchiseptica. The onset of upper respiratory disease (URD) was determined by veterinary staff based on clinical signs, including ocular or nasal discharge. Treated cats were more likely to remain Content (Incident Rate Ratio [IRR]:1.13, Confidence Interval: 0.98-1.30, P <0.0001) and less likely to be rated as Anxious or Frustrated than Control cats over a 10 day period (IRR: 0.61, 95% CI: 0.42-0.88, P =0.007). Feline secretory IgA (S-IgA) quantified in faeces by ELISA techniques, was greater for Treated than Control cats (1451 Vs 846μg/g). Within the Treatment group, S-IgA was greater for cats that sustained Contentment throughout the study period compared to cats that became Anxious or Frustrated (1846 Vs 1394μg/g). An increasing proportion of Control than Treated cats shed pathogens over time (Control 22%, 36%, 61%; Treated 35%, 26%, 32% on d 1, 4 and 10, respectively; P =0.006). Control cats were more likely to develop URD than Treated cats (HR 2.9, CI: 1.30-6.67, P =0.01). Cats that responded positively to

  15. Prophylactic treatment of flea-infested cats with an imidacloprid/flumethrin collar to forestall infection with Dipylidium caninum

    Directory of Open Access Journals (Sweden)

    Fourie Josephus J

    2012-07-01

    Full Text Available Abstract Background The objective of the study was to determine the sustained effectiveness of 10% imidacloprid (w/w and 4.5% flumethrin (w/w incorporated in a slow-release matrix collar in preventing Dipylidium caninum infection in cats following repeated laboratory-infestations with fleas infected with metacestodes. Methods Efficacy against infection with D. caninum was evaluated by infesting 16 cats with the flea Ctenocephalides felis felis infected with metacestodes of the tapeworm. Medicated collars were fitted to 8 of the cats and infestation of each cat with 200 fleas from a suitably infected batch commenced 7 days later and continued at weekly intervals until Day 28. Efficacy against fleas was evaluated 24 h after each infestation. Infection of the cats with D. caninum was verified by daily examination of the cats’ faeces and immediate surroundings for proglottids from Day 21 to Day 60. Calculation of the prophylactic effectiveness of the collars in preventing infection of the cats with D. caninum was based on the difference in the geometric mean number of scoleces recovered from the gastrointestinal tracts of collared compared to untreated cats at necropsy on Day 61. Results Efficacy of the collars against infestation of the cats with fleas was 99.9% on Day 7 and 100% at each subsequent weekly assessment. Infection of the fleas with metacestodes was ≥40% in 7 to 13 day old fleas, but progressively decreased thereafter. At necropsy all the control cats were infected with D. caninum and harboured between 19 and 346 scoleces with a geometric mean of 58.3. A single treated cat was infected and harboured 2 scoleces. Effective prevention of infection with D. caninum, based on a comparison of the geometric mean numbers of scoleces recovered from control and treated cats, was 99.7%. Conclusion The insecticidal components of the medicated collars are capable of rapidly eliminating newly-acquired infestations of fleas that are infected

  16. Hellsgate Winter Range Mitigation Project; Long-term Management Plan, Project Report 1993, Final Draft.

    Energy Technology Data Exchange (ETDEWEB)

    Berger, Matthew T.

    1994-01-01

    A study was conducted on the Hellsgate Winter Range Mitigation Project area, a 4,943 acre ranch purchased for mitigating some habitat losses associated with the original construction of Grand Coulee Dam and innundation of habitat by Lake Roosevelt. A Habitat Evaluation Procedure (HEP) study was used to determine habitat quality and quantity baseline data and future projections. Target species used in the study were sharp-tailed grouse (Tympanuchus phasianellus), mule deer (Odocoileus hemoinus), mink (Mustela vison), spotted sandpiper (Actiius colchicus), bobcat (Felis reufs), blue grouse (Dendragapus obscurus), and mourning dove (Zenaida macroura). From field data collected, limiting life values or HSI's (Habitat Suitability Index's) for each indicator species was determined for existing habitats on project lands. From this data a long term management plan was developed. This report is designed to provide guidance for the management of project lands in relation to the habitat cover types discussed and the indicator species used to evaluate these cover types. In addition, the plan discusses management actions, habitat enhancements, and tools that will be used to enhance, protect and restore habitats to desired conditions. Through planned management actions biodiversity and vegetative structure can be optimized over time to reduce or eliminate, limiting HSI values for selected wildlife on project lands.

  17. Molecular cloning, overexpression, purification, and sequence analysis of the giant panda (Ailuropoda melanoleuca) ferritin light polypeptide.

    Science.gov (United States)

    Fu, L; Hou, Y L; Ding, X; Du, Y J; Zhu, H Q; Zhang, N; Hou, W R

    2016-08-30

    The complementary DNA (cDNA) of the giant panda (Ailuropoda melanoleuca) ferritin light polypeptide (FTL) gene was successfully cloned using reverse transcription-polymerase chain reaction technology. We constructed a recombinant expression vector containing FTL cDNA and overexpressed it in Escherichia coli using pET28a plasmids. The expressed protein was then purified by nickel chelate affinity chromatography. The cloned cDNA fragment was 580 bp long and contained an open reading frame of 525 bp. The deduced protein sequence was composed of 175 amino acids and had an estimated molecular weight of 19.90 kDa, with an isoelectric point of 5.53. Topology prediction revealed one N-glycosylation site, two casein kinase II phosphorylation sites, one N-myristoylation site, two protein kinase C phosphorylation sites, and one cell attachment sequence. Alignment indicated that the nucleotide and deduced amino acid sequences are highly conserved across several mammals, including Homo sapiens, Cavia porcellus, Equus caballus, and Felis catus, among others. The FTL gene was readily expressed in E. coli, which gave rise to the accumulation of a polypeptide of the expected size (25.50 kDa, including an N-terminal polyhistidine tag).

  18. Sequencing and phylogenetic analysis of partial CXCR2 gene of Murrah buffalo

    Directory of Open Access Journals (Sweden)

    S. A. Wani

    2014-05-01

    Full Text Available Aim: Present study was carried out to sequence and phylogenetic analysis of CXCR2 gene of Murrah buffalo. Materials and Methods: For the present investigation, from a group of forty eight Murrah buffaloes (Bubalus bubalis, blood samples were collected randomly from eight animals, out of which four were healthy and four were mastitic. Results: The amplification of Interleukin-8B (IL-8B receptor gene target sequence was carried out using the primer pair in an optimized polymerase chain reaction. Partial sequencing of IL-8B receptor gene of Bubalus bubalis (Murrah has been done successfully. The sequences of IL-8B receptor gene showed 99% homology to that of Bos indicus × Bos taurus, 98% to that of Bos taurus, 97% to that of Ovis aries, 93% to that of Sus scrofa, 92% to that of Equus caballus and 90% to that of Felis catus. Conclusion: From the present study it can be concluded that the PCR amplification procedure for target region of IL-8B receptor gene yielding 459 bp products has been standardized, which yielded consistent and specific amplification. Amplification of partial IL-8B receptor gene (exon 2- 459 bp using self designed primers specific for cattle ortholog sequence signifies that the locus is conserved in cattle and buffaloes. In phylogenetic tree, the target sequence of IL-8B receptor gene of Bubalus bubalis were found to be more closely related to Bos indicus × Bos Taurus and Bos taurus than to Ovis aries and Sus scrofa.

  19. Myosin heavy chain composition of tiger (Panthera tigris) and cheetah (Acinonyx jubatus) hindlimb muscles.

    Science.gov (United States)

    Hyatt, Jon-Philippe K; Roy, Roland R; Rugg, Stuart; Talmadge, Robert J

    2010-01-01

    Felids have a wide range of locomotor activity patterns and maximal running speeds, including the very fast cheetah (Acinonyx jubatas), the roaming tiger (Panthera tigris), and the relatively sedentary domestic cat (Felis catus). As previous studies have suggested a relationship between the amount and type of activity and the myosin heavy chain (MHC) isoform composition of a muscle, we assessed the MHC isoform composition of selected hindlimb muscles from these three felid species with differing activity regimens. Using gel electrophoresis, western blotting, histochemistry, and immunohistochemistry with MHC isoform-specific antibodies, we compared the MHC composition in the tibialis anterior, medial gastrocnemius (MG), plantaris (Plt), and soleus muscles of the tiger, cheetah, and domestic cat. The soleus muscle was absent in the cheetah. At least one slow (type I) and three fast (types IIa, IIx, and IIb) MHC isoforms were present in the muscles of each felid. The tiger had a high combined percentage of the characteristically slower isoforms (MHCs I and IIa) in the MG (62%) and the Plt (86%), whereas these percentages were relatively low in the MG (44%) and Plt (55%) of the cheetah. In general, the MHC isoform characteristics of the hindlimb muscles matched the daily activity patterns of these felids: the tiger has daily demands for covering long distances, whereas the cheetah has requirements for speed and power.

  20. Helicobacter pylori-induced gastric pathology: insights from in vivo and ex vivo models

    Science.gov (United States)

    Williams, Jonathan M.

    2017-01-01

    ABSTRACT Gastric colonization with Helicobacter pylori induces diverse human pathological conditions, including superficial gastritis, peptic ulcer disease, mucosa-associated lymphoid tissue (MALT) lymphoma, and gastric adenocarcinoma and its precursors. The treatment of these conditions often relies on the eradication of H. pylori, an intervention that is increasingly difficult to achieve and that does not prevent disease progression in some contexts. There is, therefore, a pressing need to develop new experimental models of H. pylori-associated gastric pathology to support novel drug development in this field. Here, we review the current status of in vivo and ex vivo models of gastric H. pylori colonization, and of Helicobacter-induced gastric pathology, focusing on models of gastric pathology induced by H. pylori, Helicobacter felis and Helicobacter suis in rodents and large animals. We also discuss the more recent development of gastric organoid cultures from murine and human gastric tissue, as well as from human pluripotent stem cells, and the outcomes of H. pylori infection in these systems. PMID:28151409

  1. Videographic evidence of endangered species depredation by feral cat

    Science.gov (United States)

    Judge, Seth; Lippert, Jill S.; Misajon, Kathleen; Hu, Darcy; Hess, Steven C.

    2012-01-01

    Feral cats (Felis cafus) have long been implicated as nest predators of endangered 'Ua'u (Hawaiian Petrel; Pterodroma sandwichensis) on Hawaii Island, but until recently, visual confirmation has been limited by available technology. 'Ua'u nest out of view, deep inside small cavities, on alpine lava flows. During the breeding seasons of 2007 and 2008, we monitored known burrows within Hawai'i Volcanoes National Park. Digital infrared video cameras assisted in determining the breeding behaviour and nesting success at the most isolated of burrows. With 7 cameras, we collected a total of 819 videos and 89 still photographs of adult and nestling 'Ua'u at 14 burrows. Videos also confirmed the presence of rats (Rattus spp.) at 2 burrows, 'Ōmao (Myadestes obscurus) at 8 burrows, and feral cats at 6 burrows. A sequence of videos showed a feral cat taking a downy 'Ua'u chick from its burrow, representing the first direct evidence of 'Ua'u depredation by feral cat in Hawai'i. This technique provides greater understanding of feral cat behaviour in 'Ua'u colonies, which may assist in the development of more targeted management strategies to reduce nest predation on endangered insular bird species.

  2. Molecular detection of bacteria in the families Rickettsiaceae and Anaplasmataceae in northern crested caracaras (Caracara cheriway)

    Science.gov (United States)

    Erwin, John A.; Fitak, Robert R.; Dwyer, James F.; Morrison, Joan L.; Culver, Melanie

    2016-01-01

    Bacterial pathogens of the families Anaplasmataceae and Rickettsiaceae are often spread to humans or other animals from bites from infected arthropod hosts. Recently, an increasing number of studies have implicated migratory birds in the circulation of these pathogens through the spread of arthropod vectors. However, few studies have examined the potential for resident bird populations to serve as reservoirs for these zoonoses. In this study, we used nested PCRs of the GroESL and 17 kDa genes to screen for Anaplasmataceae and Rickettsiaceae, respectively, in a resident population of the northern crested caracara (Caracara cheriway) from Florida (n = 55). Additionally, a small number (n = 6) of captive individuals from Texas were included. We identified one individual (1.64%) positive for Rickettsia felis and one (1.64%) positive for Ehrlichia chaffeensis; both these individuals were from Florida. Presence of these pathogens demonstrates that these birds are potential hosts; however, the low prevalence of infections suggests that these populations likely do not function as an ecological reservoir.

  3. Use of a garbage dump by some mammal species in the Majella massif (Abruzzo, Italy

    Directory of Open Access Journals (Sweden)

    Aldo Martina

    1997-12-01

    Full Text Available Abstract In several previous works the presence of urban solid wastes in the feeding habits of some animal species has been documented. This study was carried out to discover which species visit a rubbish dump located in an area of the Majella massif. Monthly nightime direct observations, from September 1990 to May 1992, revealed the presence of dogs Canis [lupus] familiaris, domestic cats Felis [silvestris] catus, wolves Canis lupus and red foxes Vulpes vulpes. The fox was the most frequently observed species. Fox visits to the dump were distributed more continuously during the entire period of the study then the other species. An "asymmetry analysis" was carried out to find out the animals' favourite time to use the dump. The analysis produced time lag values of 02:00 A.M. to 05:00 A.M. The wolf was observed the least. During the monthly observations a similar trend between the cat and wolf came to light. The two species also visited the dump at similar times (wolf: 07:00-11:00 P.M.; cat: 05:00-10:00 P.M.. Dog visits to the dump were less continuous in comparison with other species: no preferred time was revealed. Some environmental factors, related to the geographical area and management of the dump limited waste availability. This probably had an effect on the presence of the animals.

  4. Chromosome evolution in bears: reconstructing phylogenetic relationships by cross-species chromosome painting.

    Science.gov (United States)

    Tian, Ying; Nie, Wenhui; Wang, Jinhuan; Ferguson-Smith, Malcolm A; Yang, Fengtang

    2004-01-01

    Genome-wide homology maps among dog (Canis familiaris, CFA, 2n = 78), African lion (Panthera leo, PLE, 2n = 38), clouded leopard (Neofelis nebulosa, NNE, 2n = 38) and Malayan sun bear (Helartos malayanus, HMA, 2n = 74) have been established by chromosome painting using a complete set of dog probes. In total, chromosome-specific painting probes from the 38 dog autosomes reveal 69, 69 and 73 conserved segments in African lion, clouded leopard and Malayan sun bear, respectively. The chromosomal painting results show that the African lion and clouded leopard have an identical karyotype which, in turn, is similar to that previously published for the cat (Felis catus, FCA 2n = 38). The findings confirm and extend other studies that show felids to be karyotypically conserved. In contrast, ursids, including the Malayan sun bear, have a relatively highly rearranged karyotype in comparison with other carnivores. The 2n = 74 karyotype of the Malayan sun bear, which is believed to closely resemble the ancestral karyotype of the Ursidae, could have evolved from the 2n = 42 putative ancestral carnivore karyotype by an inversion and 16 centric fissions. Independent fusions of the acrocentric ancestral chromosomes have generated the unique karyotypes of the giant panda and the spectacled bear.

  5. Analyzing the proximity to cover in a landscape of fear: a new approach applied to fine-scale habitat use by rabbits facing feral cat predation on Kerguelen archipelago

    Directory of Open Access Journals (Sweden)

    Pierrick Blanchard

    2016-03-01

    Full Text Available Although proximity to cover has been routinely considered as an explanatory variable in studies investigating prey behavioral adjustments to predation pressure, the way it shapes risk perception still remains equivocal. This paradox arises from both the ambivalent nature of cover as potentially both obstructive and protective, making its impact on risk perception complex and context-dependent, and from the choice of the proxy used to measure proximity to cover in the field, which leads to an incomplete picture of the landscape of fear experienced by the prey. Here, we study a simple predator-prey-habitat system, i.e., rabbits Oryctolagus cuniculus facing feral cat Felis catus predation on Kerguelen archipelago. We assess how cover shapes risk perception in prey and develop an easily implementable field method to improve the estimation of proximity to cover. In contrast to protocols considering the “distance to nearest cover”, we focus on the overall “area to cover”. We show that fine-scale habitat use by rabbits is clearly related to our measure, in accordance with our hypothesis of higher risk in patches with smaller area to cover in this predator-prey-habitat system. In contrast, classical measures of proximity to cover are not retained in the best predictive models of habitat use. The use of this new approach, together with a more in-depth consideration of contrasting properties of cover, could help to better understand the role of this complex yet decisive parameter for predator-prey ecology.

  6. Two geographically separated food-borne outbreaks in Sweden linked by an unusual Cryptosporidium parvum subtype, October 2010.

    Science.gov (United States)

    Gherasim, A; Lebbad, M; Insulander, M; Decraene, V; Kling, A; Hjertqvist, M; Wallensten, A

    2012-11-15

    The number of sporadic cases of Cryptosporidium identified in the Stockholm county area increased above the expected limit during October 2010. Additionally, two food-borne outbreaks of cryptosporidiosis occurred in two other Swedish cities: Umeå (4 October) and Örebro (9 October). The outbreak investigations did not reveal any responsible food item, however fresh herbs were suspected. Thirty stool samples, originating from all three events, tested positive for Cryptosporidium oocysts. Polymerase chain reaction (PCR) and subsequent restriction fragment length polymorphism (RFLP) revealed that 27 individuals were infected with C. parvum, two with C. hominis, and one with C. felis. Using sequence analysis of the GP60 glycoprotein gene, a polymorphic marker with high intra-species diversity, we identified the same C. parvum subtype IIdA24G1 in samples from both the Umeå outbreak and the Stockholm area cases, thus indicating a possible outbreak in the Stockholm area and establishing a link between these two events. C. parvum IIdA24G1 has not previously been described in connection with a food-borne outbreak. For the outbreak in Örebro, another subtype was identified: C. parvum IIdA20G1e. These findings demonstrate that subtyping C. parvum isolates using GP60 gene amplification can be used to link cases in an outbreak investigation and we recommend its use in future similar events.

  7. Social referencing and cat-human communication.

    Science.gov (United States)

    Merola, I; Lazzaroni, M; Marshall-Pescini, S; Prato-Previde, E

    2015-05-01

    Cats' (Felis catus) communicative behaviour towards humans was explored using a social referencing paradigm in the presence of a potentially frightening object. One group of cats observed their owner delivering a positive emotional message, whereas another group received a negative emotional message. The aim was to evaluate whether cats use the emotional information provided by their owners about a novel/unfamiliar object to guide their own behaviour towards it. We assessed the presence of social referencing, in terms of referential looking towards the owner (defined as looking to the owner immediately before or after looking at the object), the behavioural regulation based on the owner's emotional (positive vs negative) message (vocal and facial), and the observational conditioning following the owner's actions towards the object. Most cats (79 %) exhibited referential looking between the owner and the object, and also to some extent changed their behaviour in line with the emotional message given by the owner. Results are discussed in relation to social referencing in other species (dogs in particular) and cats' social organization and domestication history.

  8. Identification and characterisation of coding tandem repeat variants in incA gene of Chlamydophila pecorum.

    Science.gov (United States)

    Yousef Mohamad, Khalil; Rekiki, Abdessalem; Myers, Garry; Bavoil, Patrik M; Rodolakis, Annie

    2008-01-01

    Bacteria of the family Chlamydiaceae are obligate intracellular pathogens of human and animals. Chlamydophila pecorum is associated with different pathological conditions in ruminants, swine and koala. To characterize a coding tandem repeat (CTR) identified at the 3' end of incA gene of C. pecorum, 51 strains of different chlamydial species were examined. The CTR were observed in 18 of 18 tested C. pecorum isolates including symptomatic and asymptomatic animals from diverse geographical origins. The CTR were also found in two strains of C. abortus respectively isolated from faeces from a healthy ewe and from a goat belonging to asymptomatic herds, but were absent in C. abortus strains isolated from clinical disease specimens, and in tested strains of C. psittaci, C. caviae, C. felis and C. trachomatis. The number of CTR repeats is variable and encode several motifs that are rich in alanine and proline. The CTR-derived variable structure of incA, which encode the Chlamydiaceae-specific type III secreted inclusion membrane protein, IncA, may be involved in the adaptation of C. pecorum to its environment by allowing it to persist in the host cell.

  9. Enteric protozoan parasites in stray cats in Kuwait with special references to toxoplasmosis and risk factors affecting its occurrence.

    Science.gov (United States)

    Abdou, Nadra-Elwgoud M I; Al-Batel, Maha K; El-Azazy, Osama M E; Sami, Attia M; Majeed, Qais A H

    2013-08-01

    In Kuwait, stray cats were surveyed for enteric protozoan infection using fecal examination and their sera were tested for Toxoplasma gondii IgG using indirect hemagglutination test (IHAT) as well as for feline immunodeficiency virus (FIV) antibodies and feline leukaemia virus (FeLV) antibodies using ELISA. Out of 240 fecal samples examined 22 (9.2%) were found to be infected with oocysts of four species of coccidian protozoa. Isopspora felis was the most predominant enteric protozoan parasite (7.1%), followed by T. gondii (2.1%), I. rivolta (1.6), Sarcocystis was only found in one case (0.4%). Juvenile cats ( 6 months old) had higher infection rate with oocyst of enteric protozoa than older cats (p-value 0.001). Sero-survey of 240 stray cats revealed that 19.6% were positive to T. gondii IgG. Toxoplasma sero-positivity was observed in higher number of adults compared to young cats suggests that with age the risk of exposure to T. gondii increases. While concurrent retroviral infections were not found to be associated with increased risk for developing T. gondii antibodies.

  10. Current Status of Mammals and Reptiles at Hub Dam Area, Sindh / Balochistan, Pakistan

    Directory of Open Access Journals (Sweden)

    Abeda Begum

    2013-12-01

    Full Text Available During the present study in 2012, a total of twenty four mammalian species were recorded belonging to 5 orders and 10 families; out of these, 8 species are less common, 2 species are rare, while 14 species are common in Hub Dam area. Twenty five reptilian species belonging to 3 orders and 12 families were also recorded from the area. Three species of mammalian Urial (Ovis vignei, Chinkara/Indian Gazelle (Gazella bennettii and Jungle Cat (Felis chaus, one reptilian species Common Krait (Bungarus caeruleus were recorded as rare from the study area during 2012. During the present study, nine mammalian species Wild Goat/Sindh Ibex (Capra aegagrus, Urial (Ovis vignei, Chinkara/Indian Gazelle (Gazella bennettii, Indian Hedgehog (Paraechinus micropus, Cape Hare (Lepus capensis, Little Indian Field Mouse (Mus booduga, House Shrew (Sorex thibetanus, Balochistan Gerbil (Gerbillus nanus and Indian Gerbil (Tatera indica and two reptilian Warty Rock Gecko (Cyrtodactylus kachhensis kachhensis and Banded Dwarf Gecko (Tropiocolotes helenae were recorded from the area. As regards threats to mammals and reptiles, these are affected by disturbance. Efforts are being made to conserve the important mammals and reptiles particularly in the protected area especially at Hub Dam.

  11. Wild dogma: An examination of recent “evidence” for dingo regulation of invasive mesopredator release in Australia

    Institute of Scientific and Technical Information of China (English)

    Benjamin L. ALLEN; Richard M. ENGEMAN; Lee R. ALLEN

    2011-01-01

    There is growing interest in the role that apex predators play in shaping terrestrial ecosystems and maintaining trophic cascades.In line with the mesopredator release hypothesis,Australian dingoes (Canis lupus dingo and hybrids) are assumed by many to regulate the abundance of invasive mesopredators,such as red foxes Vulpes vulpes and feral cats Felis catus,thereby providing indirect benefits to various threatened vertebrates.Several recent papers have claimed to provide evidence for the biodiversity benefits of dingoes in this way.Nevertheless,in this paper we highlight several critical weaknesses in the methodological approaches used in many of these reports,including lack of consideration for seasonal and habitat differences in activity,the complication of simple track-based indices by incorporating difficult-to-meet assumptions,and a reduction in sensitivity for assessing populations by using binary measures rather than potentially continuous measures.Of the 20 studies reviewed,15 of them (75%) contained serious methodological flaws,which may partly explain the inconclusive nature of the literature investigating interactions between invasive Australian predators.We therefore assert that most of the “growing body of evidence” for mesopredator release is merely an inconclusive growing body of literature only.We encourage those interested in studying the ecological roles of dingoes relative to invasive mesopredators and native prey species to account for the factors we identify,and caution the value of studies that have not done so [Current Zoology 57 (5):568-583,2011].

  12. Adaptive Surface Reconstruction Based on Tensor Product Algebraic Splines

    Institute of Scientific and Technical Information of China (English)

    Xinghua Song; Falai Chen

    2009-01-01

    Surface reconstruction from unorganized data points is a challenging problem in Computer Aided Design and Geometric Modeling. In this paper, we extend the mathematical model proposed by Juttler and Felis (Adv. Comput. Math., 17 (2002), pp. 135-152) based on tensor product algebraic spline surfaces from fixed meshes to adaptive meshes. We start with a tensor product algebraic B-spline surface defined on an initial mesh to fit the given data based on an optimization approach. By measuring the fitting errors over each cell of the mesh, we recursively insert new knots in cells over which the errors are larger than some given threshold, and construct a new algebraic spline surface to better fit the given data locally. The algorithm terminates when the error over each cell is less than the threshold. We provide some examples to demonstrate our algorithm and compare it with Jiittler's method. Examples suggest that our method is effective and is able to produce reconstruction surfaces of high quality.AMS subject classifications: 65D17

  13. Distribution and Activity of Small Mammals on Pastoral Farmland and Forest in New Zealand

    Directory of Open Access Journals (Sweden)

    Carolyn Mary King

    2007-12-01

    Full Text Available We monitored the distribution and activity of small mammals on central North Island farmland continuously for 11 weeks in late summer and autumn 2005, using an automated monitoring device, the Scentinel®. Between 11 February and 29 April 2005 (1718 trap-nights, 1559 visits by small mammals, we documented extensive spatial and temporal variation in distribution and activity of small mammals. Hedgehogs (Erinaceus europaeus and rats (Rattus rattus were strictly nocturnal; ferrets (Mustela furo were mostly nocturnal, and feral cats (Felis catus were indifferent. The disappearance of ferrets during a standard control programme was well represented. Records of rats suddenly increased in early April, coinciding both with the removal of ferrets and with the maize harvest, which reduced cover in the fields. We repeated the trial with six Scentinels set in mixed podocarp/hardwood forest for 5 weeks in mid winter. Most (82% visits recorded between 26 May and 20 June 2005 (198 trap-nights, 690 visits by small mammals were by rats, all strictly nocturnal, but 9 visits by stoats (Mustela erminea were all diurnal.

  14. Trki ptic v stekleno pročelje poslovne stavbe v Ljubljani (osrednja Slovenija jeseni 2012/ Bird collisions with glass façade of a commercial building in Ljubljana (central Slovenia in autumn 2012

    Directory of Open Access Journals (Sweden)

    Šumrada Tanja

    2015-11-01

    Full Text Available From 28 Sep to 7 Oct 2012, bird collisions with the glass façade of a commercial building in the centre of Ljubljana were monitored. The observations lasted 45-60 minutes in the morning (7.00-10.00 hrs, around midday (11.00-14.00 hrs and in the afternoon (15.00-18.00 hrs. Behaviour of all birds and scavengers, which could potentially be looking for bird carcasses in the vicinity of the building, was noted. In 27.25 hours of observation, 16 collisions (3 resulting in death, 13 cases with birds flying away seemingly unharmed and 19 near collisions, when birds avoided the building at the last moment before collision, were recorded. The total collision rate was 0.59 collisions per hour of observation. All birds that collided with the building, except Feral Pigeon Columba livia f. domestica, were passerines, among which tits Paridae predominated (62.5% of birds that collided with the building. The glass façade functioned as a mirror, reflecting tree crowns from across the street. Data show that most collisions occurred in the middle part of the building during the morning. Among potential scavengers, domestic cat Felis domesticus and Hooded Crow Corvus cornix were observed. The latter regularly flew around the building during the observation period, possibly looking for bird carcasses.

  15. Nardilysin regulates inflammation, metaplasia, and tumors in murine stomach

    Science.gov (United States)

    Kimura, Yuto; Ikuta, Kozo; Kimura, Takeshi; Chiba, Tsutomu; Oshima, Hiroko; Oshima, Masanobu; Nishi, Eiichiro; Seno, Hiroshi

    2017-01-01

    Chronic inflammation contributes to a wide variety of human disorders. In the stomach, longstanding gastritis often results in structural alterations in the gastric mucosa, including metaplastic changes and gastric cancers. Therefore, it is important to elucidate factors that are involved in gastric inflammation. Nardilysin (N-arginine dibasic convertase; Nrdc) is a metalloendopeptidase of the M16 family that promotes ectodomain shedding of the precursor forms of various growth factors and cytokines by enhancing the protease activities of a disintegrin and metalloproteinase (ADAM) proteins. Here, we have demonstrated that Nrdc crucially regulates gastric inflammation caused by Helicobacter felis infection or forced expression of prostaglandin E2 in K19-C2mE mice. Metaplastic changes following gastric inflammation were suppressed by the deletion of Nrdc. Furthremore, the deletion of Nrdc significantly suppressed N-methyl-N-nitrosourea (MNU)-induced gastric tumorigenesis in the murine stomach. These data may lead to a global therapeutic approach against various gastric disorders by targeting Nrdc. PMID:28230087

  16. The hammock: a reservoir of allergens

    Directory of Open Access Journals (Sweden)

    Francisca X. M. Rego

    2011-01-01

    Full Text Available INTRODUCTION: Asthma affects approximately 10% of the world's population. Sensitization to allergens is an important risk factor, and exposure to allergens is associated with disease severity. METHODS: We performed skin tests to evaluate allergen sensitization to mites, cockroaches, cats, dogs, and molds in 73 asthmatic patients. Enzyme Linked Immunosorbent Assay was used to assay the mite and cockroach allergens found in dust from the bedding, hammocks, bedroom floors, living rooms, and kitchens of 29 patients and 14 controls. RESULTS: Fifty patients (68.5% had positive skin test responses. There were positive responses to D. pteronyssinus (52.0%, B. tropicalis (53.4%, T. putrescentiae (15.0%, E. maynei (12.3%, L. destructor (8.2%, B. germanica (20.5%, P. americana (21.9%, Felis catus (10.9%, C. herbarium (2.7%, A. alternata (4.1%, and P. notatun (1.3%. The exposure to mite and cockroach allergens was similar in the patients and the controls. The Dermatophagoides pteronyssinus Group 1 levels were highest in the beds and hammocks. The Blattella germanica Group 1 levels were highest in the kitchens, living rooms and hammocks. DISCUSSION: The positive skin tests to mites, cockroaches and cats were consistent with previous studies. D pteronyssinus was the most prevalent home dust mite, and hammocks were a source of allergens. To improve asthma prophylaxis, it is important to determine its association with mite allergen exposure in hammocks.

  17. Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens.

    Directory of Open Access Journals (Sweden)

    2006-05-01

    Full Text Available The recently sequenced Rickettsia felis genome revealed an unexpected plasmid carrying several genes usually associated with DNA transfer, suggesting that ancestral rickettsiae might have been endowed with a conjugation apparatus. Here we present the genome sequence of Rickettsia bellii, the earliest diverging species of known rickettsiae. The 1,552,076 base pair-long chromosome does not exhibit the colinearity observed between other rickettsia genomes, and encodes a complete set of putative conjugal DNA transfer genes most similar to homologues found in Protochlamydia amoebophila UWE25, an obligate symbiont of amoebae. The genome exhibits many other genes highly similar to homologues in intracellular bacteria of amoebae. We sought and observed sex pili-like cell surface appendages for R. bellii. We also found that R. bellii very efficiently multiplies in the nucleus of eukaryotic cells and survives in the phagocytic amoeba, Acanthamoeba polyphaga. These results suggest that amoeba-like ancestral protozoa could have served as a genetic "melting pot" where the ancestors of rickettsiae and other bacteria promiscuously exchanged genes, eventually leading to their adaptation to the intracellular lifestyle within eukaryotic cells.

  18. Isolation of Bartonella henselae, Bartonella koehlerae subsp. koehlerae, Bartonella koehlerae subsp. bothieri and a new subspecies of B. koehlerae from free-ranging lions (Panthera leo) from South Africa, cheetahs (Acinonyx jubatus) from Namibia and captive cheetahs from California.

    Science.gov (United States)

    Molia, S; Kasten, R W; Stuckey, M J; Boulouis, H J; Allen, J; Borgo, G M; Koehler, J E; Chang, C C; Chomel, B B

    2016-11-01

    Bartonellae are blood- and vector-borne Gram-negative bacteria, recognized as emerging pathogens. Whole-blood samples were collected from 58 free-ranging lions (Panthera leo) in South Africa and 17 cheetahs (Acinonyx jubatus) from Namibia. Blood samples were also collected from 11 cheetahs (more than once for some of them) at the San Diego Wildlife Safari Park. Bacteria were isolated from the blood of three (5%) lions, one (6%) Namibian cheetah and eight (73%) cheetahs from California. The lion Bartonella isolates were identified as B. henselae (two isolates) and B. koehlerae subsp. koehlerae. The Namibian cheetah strain was close but distinct from isolates from North American wild felids and clustered between B. henselae and B. koehlerae. It should be considered as a new subspecies of B. koehlerae. All the Californian semi-captive cheetah isolates were different from B. henselae or B. koehlerae subsp. koehlerae and from the Namibian cheetah isolate. They were also distinct from the strains isolated from Californian mountain lions (Felis concolor) and clustered with strains of B. koehlerae subsp. bothieri isolated from free-ranging bobcats (Lynx rufus) in California. Therefore, it is likely that these captive cheetahs became infected by an indigenous strain for which bobcats are the natural reservoir.

  19. 宁夏沙坡头自然保护区兽类区系研究

    Institute of Scientific and Technical Information of China (English)

    钟泓; 徐怀寿; 黄族豪; 刘发

    2008-01-01

    沙坡头国家级自然保护区位于腾格里沙漠东南缘,保护区内共有兽类21种,隶属于6目11科19属,以啮齿目种类最多,占52.38%。荒漠猫(Felis bieti)、猞猁(Lynx lynx)、鹅喉羚(Gazella subgutturosa)和岩羊(Pseudoisnayaur)是国家Ⅱ级重点保护动物。荒漠猫和猞猁列入《濒危野生动植物种国际贸易公约》附录Ⅱ。沙坡头保护区的兽类区系以古北界最多,占85.71%;缺乏东洋界种类。沙坡头地区的兽类区系形成于第三纪晚期。

  20. The carnivore remains from the Sima de los Huesos Middle Pleistocene site (Sierra de Atapuerca, Spain).

    Science.gov (United States)

    García, N; Arsuaga, J L; Torres, T

    1997-01-01

    Remains of carnivores from the Sima de los Huesos site representing at least 158 adult individuals of a primitive (i.e., not very speleoid) form of Ursus deningeri Von Reichenau 1906, have been recovered through the 1995 field season. These new finds extend our knowledge of this group in the Sierra de Atapuerca Middle Pleistocene. Material previously classified as Cuoninae indet, is now assigned to Canis lupus and a third metatarsal assigned in 1987 to Panthera of gombaszoegensis, is in our opinion only attributable to Panthera sp. The family Mustelidae is added to the faunal list and includes Martes sp. and a smaller species. The presence of Panthera leo cf. fossilis, Lynx pardina spelaea and Felis silvestris, is confirmed. The presence of a not very speloid Ursus deningeri, together with the rest of the carnivore assemblage, points to a not very late Middle Pleistocene age, i.e., oxygen isotope stage 7 or older. Relative frequencies of skeletal elements for the bear and fox samples are without major biases. The age structure of the bear sample, based on dental wear stages, does not follow the typical hibernation mortality profile and resembles a catastrophic profile. The site was not a natal or refuge den. The hypothesis that the site was a natural trap is the most plausible. If the Sima de los Huesos functioned as a natural trap (without an egress out), the human accumulation cannot be attributed to carnivore: activities and must be explained differently.