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Sample records for bagre ariopsis felis

  1. Weight-Length Relationships in Gafftopsail Catfish (Bagre marinus) and Hardhead Catfish (Ariopsis felis) in Louisiana Waters

    OpenAIRE

    Courtney, Joshua; Klinkmann, Taylor; Torano, Joseph; 2; Courtney, Michael

    2012-01-01

    In spite of the abundance and commercial importance of these two species, there is little published weight-length data for the gafftopsail catfish (Bagre marinus) and hardhead catfish (Ariopsis felis). For this study 84 catfish were caught (hook and line) from the Calcasieu Estuary in Southwest Louisiana near the Gulf of Mexico and estuaries and near shore waters close to bayou Lafourche. Using least squares regression, best fit curves were determined for weight (W) vs. total length (L) relat...

  2. Metales pesados en tejido muscular del bagre Ariopsis felis en el sur del golfo de México (2001-2004) Heavy metals in muscular tissue of the catfish, Ariopsis felis, in the southern Gulfof México (2001-2004)

    OpenAIRE

    Felipe Vázquez; Tomás R Florville-Alejandre; Miguel Herrera; Luz María Díaz de León

    2008-01-01

    Se analizó el contenido de metales pesados en tejido muscular del bagre, Ariopsis felis en el sur del golfo de México durante el período 2001-2004. La investigación fue efectuada buscando establecer un marco de referencia ambiental para este organismo. La concentración metálica siguió el orden: Hg < Co < Pb < Ni < V < Cr. Fue observado un decremento en contenido de cobalto y vanadio, incremento en contenido de níquel, mercurio y cromo, y ausencia de estos comportamientos para contenido de plo...

  3. Metales pesados en tejido muscular del bagre Ariopsis felis en el sur del golfo de México (2001-2004 Heavy metals in muscular tissue of the catfish, Ariopsis felis, in the southern Gulfof México (2001-2004

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    Felipe Vázquez

    2008-01-01

    Full Text Available Se analizó el contenido de metales pesados en tejido muscular del bagre, Ariopsis felis en el sur del golfo de México durante el período 2001-2004. La investigación fue efectuada buscando establecer un marco de referencia ambiental para este organismo. La concentración metálica siguió el orden: Hg We analyzed the heavy metal content in the muscular tissue of the catfish, Ariopsis felis, in the southern Gulf of México between 2001 and 2004. The research was done in order to establish an environmental frame of reference for this organism. The metal concentration was as follows: Hg < Co < Pb < Ni < V < Cr. Cobalt and vanadium contents were found to decrease and those of nickel, mercury, and chromium to ulerease; however, neither of these trends was observed for the lead content. One-way analyses of variance con-firm significant temporal variation only for cobalt, mercury, lead, and vanadium. Significant linear correlation coefficients (p ≤ 0.05 were found for Co-V, Cr-Ni, Cr-Pb, Co-Hg, Ni-V, and V-Pb. The first three associa-tions showed positive correlations, whereas the remaining ones had negative correlations. A factor analysis grouped the studied metals depending on their origins. The metal levels found in the muscular tissue of A. felis from the southern Gulf of México were lower than those set by national and international regulations.

  4. Phylogenetic relationships among five marine Catfish species (Pisces: Ariidae from Mexico Relaciones filogenéticas entre cinco especies de bagres (Pisces: Ariidae de México

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    Guadalupe Tenorio-Colín

    2010-01-01

    Full Text Available The systematics of the marine catfish of the family Ariidae is controversial because at the present time the number of species and genera in the family, or their relationships, remain uncertain. Phylogenetic relationships among five representative species of marine catfish of the family Ariidae from both the Pacific and the Atlantic coasts of Mexico were assessed by the analysis of the variability in 21 alloenzymatic loci, and by the comparison of the electrophoretic patterns of whole muscle proteins. Interspecific genetic divergence levels obtained by both electrophoretic methods showed a clear separation among the genera Cathorops, Bagre and Ariopsis, as well as in the studied species of Cathorops and Ariopsis, with Bagre marinus showing a greater genetic similarity with the Ariopsis group. Finally, our results contribute to the definition of the presence of this species in the coasts of the Gulf of Mexico.La sistemática de los bagres marinos pertenecientes a la familia Ariidae es controversial, porque hasta la actualidad, no se conoce con exactitud el número de especies y géneros existentes en la familia, ni las relaciones que se establecen entre ellos. En el presente trabajo se estudiaron las relaciones filogenéticas entre cinco especies representativas de bagres marinos de la familia Ariidae, de ambas costas del Pacífico y del Atlántico Mexicano. El mencionado análisis se llevó a cabo mediante el análisis de la variabilidad en 21 loci aloenzimáticos y a través de la comparación de patrones electroforéticos de proteínas totales de músculo. Los niveles de divergencia interespecífica obtenidos por ambos métodos electroforéticos mostraron una clara separación entre los géneros Cathorops, Bagre y Ariopsis, así como entre las especies estudiadas de Cathorops y Ariopsis con Bagre marinus, mostrando una mayor similitud genética con el grupo Ariopsis. Los resultados obtenidos en este estudio contribuyen al establecimiento de la

  5. Filogenia de los bagres marinos (Siluriformes: Ariidae del nuevo mundo

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    Betancur Rodríguez Ricardo

    2004-12-01

    Full Text Available La familia Ariidae comprende peces marinos, estuarinos y dulceacuícolas, distribuidos en aguas tropicales y subtropicales de todos los continentes. La nomenclatura específica y genérica en el grupo es caótica y no hay continuidad de su uso en la literatura. En este trabajo se presenta una filogenia robusta para 37-41 especies de ariidos americanos y dos especies del Viejo Mundo, basada en un máximo de 2.922 caracteres del genoma mitocondrial (citocromo b, ATP sintetasa 8 y 6, 12S y 16S y 978 del nuclear (gen activador de la recombinación 2, por reconstrucción con los criterios de máxima parsimonia (MP e inferencia bayesiana (BI. Adicionalmente, se complementó el trabajo morfológico previo incluyendo siete especies y 11 caracteres adicionales, para una matriz final de 26 especies y 56 caracteres. Las secuencias mitocondriales proporcionaron alta resolución a diferentes niveles (subfamiliar, genérico y específico. Aunque BI recuperó en más ocasiones nodos débiles incongruentes con otros conjuntos de datos, mostró mayor sensibilidad que MP a la escasa señal filogenética del locus nuclear, el cual ofreció poca
    resolución. La evidencia morfológica fue congruente con las hipótesis moleculares a nivel subfamiliar, genérico y en gran medida intergenérico; las discrepancias más marcadas entre ambos
    enfoques se dan al interior del género Bagre. Las filogenias obtenidas concuerdan todas con la división de Ariidae en dos subfamilias: Ariinae y el grupo de los Galeichthyinos, el cual requiere
    estatus nomenclatural. Se corroboró la validez, y en gran medida la monofilia de los géneros Ariopsis, Bagre, Cathorops, Galeichthys, Potamarius y Selenaspis (incluyendo a S. proops y S. parkeri. El
    género Arius sensu lato no es monofilético y no debe ser usado para referir a las especies americanas. El linaje 'Arius A' conforma un grupo monofilético anfiamericano al cual debe atribuírsele un nombre gen

  6. Rickettsia felis infection in cat fleas Ctenocephalides felis felis

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    Mauricio C. Horta

    2010-10-01

    Full Text Available The present study evaluated the rickettsial infection in a laboratory colony of cat fleas, Ctenocephalides felis felis (Bouche in Brazil. All flea samples (30 eggs, 30 larvae, 30 cocoons, 30 males, and 30 females tested by polymerase chain reaction (PCR were shown to contain rickettsial DNA. PCR products, corresponding to the rickettsial gltA, htrA, ompA and ompB gene partial sequences were sequenced and showed to correspond to Rickettsia felis, indicating that the flea colony was 100% infected by R. felis. The immunofluorescence assay (IFA showed the presence of R. felis-reactive antibodies in blood sera of 7 (87.5% out of 8 cats that were regularly used to feed the flea colony. From 15 humans that used to work with the flea colony in the laboratory, 6 (40.0% reacted positively to R. felis by IFA. Reactive feline and human sera showed low endpoint titers against R. felis, varying from 64 to 256. With the exception of one human serum, all R. felis-reactive sera were also reactive to Rickettsia rickettsii and/or Rickettsia parkeri antigens at similar titers to R. felis. The single human serum that was reactive solely to R. felis had an endpoint titer of 256, indicating that this person was infected by R. felis.

  7. Rickettsia felis infection in cat fleas Ctenocephalides felis felis

    OpenAIRE

    Horta, Mauricio C.; Fabio B. Scott; Thaís R. Correia; Julio I. Fernandes; Leonardo J. Richtzenhain; Labruna, Marcelo B.

    2010-01-01

    The present study evaluated the rickettsial infection in a laboratory colony of cat fleas, Ctenocephalides felis felis (Bouche) in Brazil. All flea samples (30 eggs, 30 larvae, 30 cocoons, 30 males, and 30 females) tested by polymerase chain reaction (PCR) were shown to contain rickettsial DNA. PCR products, corresponding to the rickettsial gltA, htrA, ompA and ompB gene partial sequences were sequenced and showed to correspond to Rickettsia felis, indicating that the flea colony was 100% inf...

  8. Revision of the amphiamerican Neotetraonchus Bravo-Hollis, 1968 (Monogenoidea: Dactylogyridae), with a description of N. vegrandis n. sp. from the gill lamellae of the blue sea catfish Ariopsis guatemalensis (Siluriformes: Ariidae) off the Pacific Coast of Mexico.

    Science.gov (United States)

    Kritsky, Delane C; Mendoza-Franco, Edgar F; Bullard, Stephen A; Vidal-Martínez, Victor M

    2009-09-01

    Neotetraonchus Bravo-Hollis, 1968 is revised and reassigned to the Dactylogyridae Bychowsky, 1933 based on examinations of specimens representing four species from the gill lamellae of sea catfishes (Ariidae). The monotypic Neotetraonchidae Bravo-Hollis, 1968 is placed in synonymy with the Dactylogyridae. Neotetraonchus bychowskyi Bravo-Hollis, 1968 (type-species), is redescribed from the tete sea catfish Ariopsis seemanni (Günther) (type-host) in the eastern Pacific Ocean off Panama (new geographical record). Neotetraonchus vegrandis n. sp. is described from the blue sea catfish A. guatemalensis (Günther) off the Pacific Coast of Mexico. Neotetraonchus bravohollisae Paperna, 1977 is redescribed from the hardhead sea catfish A. felis (L.) in the Gulf of Mexico off the Yucatan Peninsula. Neotetraonchus felis (Hargis, 1955) Paperna, 1977 is redescribed from A. felis in the Gulf of Mexico off Mississippi and the Yucatan Peninsula (new geographical record). Morphological similarities between species of Neotetraonchus suggest the likely presence of geminate species pairs flanking the Isthmus of Panama. PMID:19633926

  9. Apuntes sobre la reproduccion de algunos Bagres marinos

    NARCIS (Netherlands)

    Luengo, José A.

    1973-01-01

    Mouthbreeding in the male, and modifications of the pelvic fins of the female are recorded for the first time in Selenaspis herzbergii. The pelvic girdle of Selenaspis herzbergii is compared with those of Sciadeichthys proops, Arius spixii, and Bagre marinus. Data are given on eggs and fry in the bu

  10. On Felis badia Gray

    NARCIS (Netherlands)

    Jentink, F.A.

    1901-01-01

    Among some very commonly known mammals, presented to the Leyden Museum by the well-known Dutch Borneoexplorer Dr. Nieuwenhuis, I found a fine Cat quite distinct from all other cats I ever saw; it has a size somewhat larger than Felis planiceps, a small head like that cat, a much longer tail and a mu

  11. Rickettsia felis in Xenopsylla cheopis, Java, Indonesia

    OpenAIRE

    Jiang, Ju; Soeatmadji, Djoko W.; Henry, Katherine M; Ratiwayanto, Sutanti; Michael J. Bangs; Richards, Allen L.

    2006-01-01

    Rickettsia typhi and R. felis, etiologic agents of murine typhus and fleaborne spotted fever, respectively, were detected in Oriental rat fleas (Xenopsylla cheopis) collected from rodents and shrews in Java, Indonesia. We describe the first evidence of R. felis in Indonesia and naturally occurring R. felis in Oriental rat fleas.

  12. Cat Scratch Disease: the Rare Role of Afipia felis

    OpenAIRE

    Giladi, Michael; Avidor, Boaz; Kletter, Yehudith; Abulafia, Suzy; Slater, Leonard N.; Welch, David F.; Brenner, Don J.; Steigerwalt, Arnold G.; Whitney, Anne M.; Ephros, Moshe

    1998-01-01

    Since its isolation in 1988, Afipia felis has been associated with cat scratch disease (CSD) in only one report and its role in CSD has been questioned. We have cultured A. felis from a lymph node of a patient with CSD. 16S rRNA gene sequencing, DNA relatedness studies, fatty acid analysis, and PCR of the A. felis ferredoxin gene showed that the isolate is identical to the previously reported A. felis isolate. To determine the role of A. felis in CSD, PCR of the 16S rRNA gene followed by hybr...

  13. Rickettsia felis, an emerging flea-transmitted human pathogen

    Directory of Open Access Journals (Sweden)

    Stephen Graves

    2011-07-01

    Full Text Available Rickettsia felis was first recognised two decades ago and has now been described as endemic to all continents except Antarctica. The rickettsiosis caused by R. felis is known as flea-borne spotted fever or cat-flea typhus. The large number of arthropod species found to harbour R. felis and that may act as potential vectors support the view that it is a pan-global microbe. The main arthropod reservoir and vector is the cat flea, Ctenocephalides felis, yet more than 20 other species of fleas, ticks, and mites species have been reported to harbour R. felis. Few bacterial pathogens of humans have been found associated with such a diverse range of invertebrates. With the projected increase in global temperature over the next century, there is concern that changes to the ecology and distribution of R. felis vectors may adversely impact public health.

  14. Efecto de tres tipos de presas vivas en la larvicultura de bagre blanco (Sorubim cuspicaudus

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    Martha Prieto-Guevara

    2013-11-01

    Full Text Available Objetivo. Evaluar el efecto de diferentes presas vivas en la larvicultura de bagre blanco (Sorubim cuspicaudus. Materiales y métodos. Al inicio de la alimentación exógena de Sorubim cuspicaudus, se ofreció zooplancton producido en mesocosmos (T1, zooplancton silvestre (T2 y nauplios de Artemia (T3, en concentración de 10.000 zoop/L, dos veces al día, durante seis días. Se utilizaron 18 acuarios de cinco litros de volumen útil, con densidad de 25 Larvas/L, seis réplicas por tratamiento en un diseño al azar. Se estimaron la ganancia en peso (Gp y longitud (Gl, tasa de crecimiento específico (G, sobrevivencia (S, resistencia al estrés (Re, mortalidad acumulada (Ma y mortalidad por canibalismo (Mc. Resultados. Las larvas alimentadas con mesocosmos presentaron la mayor sobrevivencia (81.3±15.9%; aunque el mejor crecimiento lo presentaron las larvas alimentadas con zooplancton silvestre (T2 las cuales presentaron la mayor mortalidad (42.0±10.7% y la menor resistencia al estrés (30.0±33.0%. El canibalismo se observó en todos los tratamientos, oscilando entre 4.0 (T2 y 14.3% (T1 sin diferencias significativas entre estos valores (p>0.05. Conclusiones. El uso de zooplancton producido bajo condiciones controladas permitió una alta sobrevivencia, adecuado desempeño y resistencia de las larvas, perfilándose como alternativa viable en la primera alimentación de bagre blanco.

  15. Rickettsia felis, an emerging flea-transmitted human pathogen

    OpenAIRE

    Abdad, Mohammad Yazid; Stenos, John; Graves, Stephen

    2011-01-01

    Rickettsia felis was first recognised two decades ago and has now been described as endemic to all continents except Antarctica. The rickettsiosis caused by R. felis is known as flea-borne spotted fever or cat-flea typhus. The large number of arthropod species found to harbour R. felis, and be potential vectors, supports the view that it is a pan-global microbe. The main arthropod reservoir and vector is the cat flea, Ctenocephalides felis, yet a further twenty other species of fleas, ticks a...

  16. Rickettsia felis as Emergent Global Threat for Humans

    Science.gov (United States)

    Pérez-Osorio, Carlos E.; Zavala-Velázquez, Jorge E.; León, Juan José Arias

    2008-01-01

    Rickettsia felis is an emergent pathogen belonging to transitional group rickettsiae. First described in 1990, R. felis infections have been reported to occur worldwide in fleas, mammals, and humans. Because clinical signs of the illness are similar to those of murine typhus and other febrile illnesses such as dengue, the infection in humans is likely underestimated. R. felis has been found throughout the world in several types of ectoparasites; cat fleas appear to be the most common vectors. R. felis infection should be considered an emergent threat to human health. PMID:18598619

  17. Biomarcadores histológicos em duas espécies de bagres estuarinos da Costa Maranhense, Brasil

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    D.B.P.Sousa

    2013-04-01

    Full Text Available Neste estudo objetivou-se validar lesões branquiais como biomarcadores em peixes de importância econômica (Sciades herzbergii e Bagre bagre capturados pela pesca artesanal em dois locais diferenciados da Baía de São Marcos, MA, a fim de selecionar um táxon como bioindicador capaz de ser utilizado em programas de biomonitoramento. O primeiro ponto (S1 foi utilizado como uma área de referência - Ilha dos Caranguejos -, e o segundo ponto (S2 foi considerado potencialmente impactado - Complexo Portuário de São Luís, MA. Encontraram-se várias alterações histológicas para as duas espécies em S2, destacando-se: estreitamento lamelar, teleangectasia, fusão e descolamento do epitélio da lamela secundária. Lesões branquiais em B. bagre coletados em S1 também foram observadas, não sendo possível diferenciar os indivíduos das duas áreas analisadas. Os dados indicam que S. herzbergii é um bioindicador mais apropriado para análise de biomarcadores de contaminação aquática.

  18. Mycoplasma felis as a cause of pleuritis in horses.

    Science.gov (United States)

    Ogilvie, T H; Rosendal, S; Blackwell, T E; Rostkowski, C M; Julian, R J; Ruhnke, L

    1983-06-15

    Mycoplasma felis was the only organism recovered from the thoracic cavity of a horse with pleuritis. Large numbers of mildly degenerative neutrophils were in the pleural fluid. The horse developed a serologic response to M felis and recovered during hospitalization. Experimentally, a pony was inoculated in the thoracic cavity with a pure culture of the M felis isolate suspended in the pony's serum. A control pony was inoculated with serum only. Within 48 hours, the principal pony developed fever, increased respiratory rate, pleural effusion, and signs of pain. A highly cellular exudate with nondegenerative neutrophils and large numbers of M felis was recovered from the thoracic cavity. The control pony remained normal. The principal pony developed an antibody response to M felis. The control pony did not. Fourteen days after inoculation, both ponies were euthanatized. Necropsy revealed pleural inflammation in the principal pony. Pleural lesions were not found in the control pony. PMID:6874502

  19. Prevalence and infection load dynamics of Rickettsia felis in actively feeding cat fleas.

    Directory of Open Access Journals (Sweden)

    Kathryn E Reif

    Full Text Available BACKGROUND: Rickettsia felis is a flea-associated rickettsial pathogen recurrently identified in both colonized and wild-caught cat fleas, Ctenocephalides felis. We hypothesized that within colonized fleas, the intimate relationship between R. felis and C. felis allows for the coordination of rickettsial replication and metabolically active periods during flea bloodmeal acquisition and oogenesis. METHODOLOGY/PRINCIPAL FINDINGS: A quantitative real-time PCR assay was developed to quantify R. felis in actively feeding R. felis-infected fleas. In three separate trials, fleas were allowed to feed on cats, and a mean of 3.9x10(6 R. felis 17-kDa gene copies was detected for each flea. A distinct R. felis infection pattern was not observed in fleas during nine consecutive days of bloodfeeding. However, an inverse correlation between the prevalence of R. felis-infection, which ranged from 96% in Trial 1 to 35% in Trial 3, and the R. felis-infection load in individual fleas was identified. Expression of R. felis-infection load as a ratio of R. felis/C. felis genes confirmed that fleas in Trial 3 had significantly greater rickettsial loads than those in Trial 1. CONCLUSION/SIGNIFICANCE: Examining rickettsial infection dynamics in the flea vector will further elucidate the intimate relationship between R. felis and C. felis, and facilitate a more accurate understanding of the ecology and epidemiology of R. felis transmission in nature.

  20. Mycoplasma felis pleuritis in two show-jumper horses.

    Science.gov (United States)

    Hoffman, A M; Baird, J D; Kloeze, H J; Rosendal, S; Bell, M

    1992-04-01

    Mycoplasma felis was identified as the cause of acute pleuritis in 2 show-jumping horses. The pleural exudate was proteinaceous, contained large numbers of neutrophils, and had a markedly increased lactate concentration. M. felis was isolated in pure culture from pleural fluid. Rising serum antibody titers to M. felis as well as a precipitous decline in titers to equine influenza virus were demonstrated in both horses. Pleural effusion in both horses and a pneumothorax detected in one of the horses resolved following a single drainage of pleural fluid and intravenous fluid, antibiotic, and analgesic therapy. PMID:1623728

  1. Integrated morphological and molecular identification of cat fleas (Ctenocephalides felis) and dog fleas (Ctenocephalides canis) vectoring Rickettsia felis in central Europe.

    Science.gov (United States)

    Lawrence, Andrea L; Hii, Sze-Fui; Jirsová, Dagmar; Panáková, Lucia; Ionică, Angela M; Gilchrist, Katrina; Modrý, David; Mihalca, Andrei D; Webb, Cameron E; Traub, Rebecca J; Šlapeta, Jan

    2015-06-15

    Fleas of the genus Ctenocephalides are the most common ectoparasites infesting dogs and cats world-wide. The species Ctenocephalides felis and Ctenocephalides canis are competent vectors for zoonotic pathogens such as Rickettsia felis and Bartonella spp. Improved knowledge on the diversity and phylogenetics of fleas is important for understanding flea-borne pathogen transmission cycles. Fleas infesting privately owned dogs and cats from the Czech Republic (n=97) and Romania (n=66) were subjected to morphological and molecular identification and phylogenetic analysis. There were a total of 59 (60.82%) cat fleas (Ctenocephalides felis felis), 30 (30.93%) dog fleas (Ctenocephalides canis), 7 (7.22%) European chicken fleas (Ceratophyllus gallinae) and 1 (1.03%) northern rat flea (Nosopsyllus fasciatus) collected in the Czech Republic. Both C. canis and C. felis felis were identified in Romania. Mitochondrial DNA sequencing at the cox1 gene on a cohort of 40 fleas revealed the cosmopolitan C. felis felis clade represented by cox1 haplotype 1 is present in the Czech Republic. A new C. felis felis clade from both the Czech Republic and Romania is also reported. A high proportion of C. canis was observed from dogs and cats in the current study and phylogeny revealed that C. canis forms a sister clade to the oriental cat flea Ctenocephalides orientis (syn. C. felis orientis). Out of 33 fleas tested, representing C. felis felis, C. canis and Ce. gallinae, 7 (21.2%) were positive for R. felis using diagnostic real-time PCR targeting the gltA gene and a conventional PCR targeting the ompB gene. No samples tested positive for Bartonella spp. using a diagnostic real-time PCR assay targeting ssrA gene. This study confirms high genetic diversity of C. felis felis globally and serves as a foundation to understand the implication for zoonotic disease carriage and transmission by the flea genus Ctenocephalides.

  2. Prevalence and Infection Load Dynamics of Rickettsia felis in Actively Feeding Cat Fleas

    OpenAIRE

    Reif, Kathryn E.; Stout, Rhett W.; Gretchen C Henry; Lane D Foil; Macaluso, Kevin R.

    2008-01-01

    Background Rickettsia felis is a flea-associated rickettsial pathogen recurrently identified in both colonized and wild-caught cat fleas, Ctenocephalides felis. We hypothesized that within colonized fleas, the intimate relationship between R. felis and C. felis allows for the coordination of rickettsial replication and metabolically active periods during flea bloodmeal acquisition and oogenesis. Methodology/Principal Findings A quantitative real-time PCR assay was developed to quantify R. fel...

  3. HISTOLOGÍA Y MORFOMETRÍA DEL SISTEMA DIGESTIVO DEL SILÚRIDO BAGRE TIGRITO (Pimelodus pictus)

    OpenAIRE

    CM Olaya; CH Ovalle; E. Gómez; D. Rodríguez; ML Caldas; H. Hurtado

    2007-01-01

    El bagre tigrito Pimelodus pictus es un pez ornamental con gran aceptación en el mercado acuarista internacional, sin embargo, se desconocen muchos aspectos de la biología básica de esta especie. Por tal motivo, se realizó un estudio histológico y morfométrico del sistema digestivo a Àn de contribuir al diseño de futuras dietas. Se sacriÀcaron 7 individuos adultos de P. pictus de 10 ± 0,5 cm de longitud total con una sobredosis de MS-222 (0,5 g/l). Los especímenes se Àjaron en formaldehído al...

  4. Histología y morfometría del sistema digestivo del silúrido bagre tigrito (pimelodus pictus)

    OpenAIRE

    Olaya, C. M.; Ovalle, C. H.; Gómez Ramírez, E.; Rodríguez Caicedo, D.; Caldas Martínez, M. L.; Hurtado Giraldo, H.

    2009-01-01

    El bagre tigrito Pimelodus pictus es un pez ornamental con gran aceptación en el mercado acuarista internacional, sin embargo, se desconocen muchos aspectos de la biología básica de esta especie. Por tal motivo, se realizó un estudio histológico y morfométrico del sistema digestivo a fin de contribuir al diseño de futuras dietas. Se sacrificaron 7 individuos adultos de P. pictus de 10 ± 0,5 cm de longitud total con una sobredosis de MS-222 (0,5 g/l). Los especímenes se fijaron en formaldehído...

  5. South American pemphigus foliaceus: study of an epidemic in El Bagre and Nechi, Colombia 1982 to 1986.

    Science.gov (United States)

    Robledo, M A; Prada, S; Jaramillo, D; Leon, W

    1988-06-01

    Between 1982 and 1986 we have had the chance to study 21 patients with pemphigus foliaceus of the 'fogo selvagem' type. The patients came from El Bagre and Nechi, rural areas of Colombia with some gold mining. This is the first outbreak of South American pemphigus foliaceus reported in Colombia. The majority of the patients were mestizo men, who worked as farmers or miners or both, with an average age of 44. Five patients were relatives. Eleven patients (52%) had mild disease, three (14%) moderate disease and seven (33%) severe disease. During hospitalization, three patients died due to complications or as a result of immunosuppressive treatment. Of the remaining 18 patients, 10 were in remission with treatment, one was in remission without treatment, while no information was available on the remaining seven. PMID:3401411

  6. Optical properties of infrared FELs from the FELI Facility II

    Energy Technology Data Exchange (ETDEWEB)

    Saeki, K.; Okuma, S.; Oshita, E. [Free Electron Laser Institute, Osaka (Japan)] [and others

    1995-12-31

    The FELI Facility II has succeeded in infrared FEL oscillation at 1.91 {mu} m using a 68-MeV, 40-A electron beam from the FELI S-band linac in February 27, 1995. The FELI Facility II is composed of a 3-m vertical type undulator ({lambda}u=3.8cm, N=78, Km a x=1.4, gap length {ge}20mm) and a 6.72-m optical cavity. It can cover the wavelength range of 1-5{mu}m. The FELs can be delivered from the optical cavity to the diagnostics room through a 40-m evacuated optical pipeline. Wavelength and cavity length dependences of optical properties such as peak power, average power, spectrum width, FEL macropulse, FEL transverse profile are reported.

  7. Monitoring water turbidity and surface suspended sediment concentration of the Bagre Reservoir (Burkina Faso) using MODIS and field reflectance data

    Science.gov (United States)

    Robert, Elodie; Grippa, Manuela; Kergoat, Laurent; Pinet, Sylvain; Gal, Laetitia; Cochonneau, Gérard; Martinez, Jean-Michel

    2016-10-01

    Monitoring turbidity and Surface Suspended Sediment Concentration (SSSC) of inland waters is essential to address several important issues: erosion, sediment transport and deposition throughout watersheds, reservoir siltation, water pollution, human health risks, etc. This is especially important in regions with limited conventional monitoring capacities such as West Africa. In this study, we explore the use of Moderate Resolution Imaging Spectroradiometer data (MODIS, MOD09Q1 and MYD09Q1 products, red (R) and near infrared (NIR) bands) to monitor turbidity and SSSC for the Bagre Reservoir in Burkina Faso. High values ​​of these parameters associated with high spatial and temporal variability potentially challenge the methodologies developed so far for less turbid waters. Field measurements (turbidity, SSSC, radiometry) are used to evaluate different radiometric indices. The NIR/R ratio is found to be the most suited to retrieve SSSC and turbidity for both in-situ spectoradiometer measurements and satellite reflectance from MODIS. The spatio temporal variability of MODIS NIR/R together with rainfall estimated by the Tropical Rainforest Measuring Mission (TRMM) and altimetry data from Jason-2 is analyzed over the Bagre Reservoir for the 2000-2015 period. It is found that rain events of the early rainy season (February-March) through mid-rainy season (August) are decisive in triggering turbidity increase. Sediment transport is observed in the reservoir from upstream to downstream between June and September. Furthermore, a significant increase of 19% in turbidity values is observed between 2000 and 2015, mainly for the July to December period. It is especially well marked for August, with the central and downstream areas showing the largest increase. The most probable hypothesis to explain this evolution is a change in land use, and particularly an increase in the amount of bare soils, which enhances particle transport by runoff.

  8. Avaliação de diferentes dietas merídicas sobre a emergência de adultos de Ctenocephalides felis felis (Siphonaptera : Pulicidae Evaluation of different meridic diets upon adult emergence of Ctenocephalides felis felis (Siphonaptera: Pulicidae

    Directory of Open Access Journals (Sweden)

    Vanessa Paulino da Cruz Vieira

    2010-03-01

    Full Text Available O objetivo do presente trabalho foi avaliar a influência de dietas merídicas compostas por sangue de diferentes espécies animais sobre a emergência de adultos da pulga Ctenocephalides felis felis. Foram utilizadas seis dietas artificiais contendo areia e sangue desidratado de cão, boi, coelho e galinha, acrescidas ou não com farelo de trigo. Foram realizadas seis repetições contendo dez ovos de C. f. felis para cada dieta. Após 25 dias de incubação, os ovos foram quantificados e avaliados quanto à emergência de adultos. O número de pulgas emergidas para dietas com sangue de boi, cão, coelho e galinha, com areia e farelo de trigo, foi de aproximadamente oito pulgas. Para as dietas contendo apenas sangue de boi ou de cão e areia, essa emergência foi de aproximadamente uma pulga para ambas. Conclui-se que a origem do sangue empregado na elaboração da dieta não interfere significativamente no percentual de emergência de adultos de C. f. felis. Portanto, pode-se optar pela espécie animal disponível para o preparo da dieta artificial, aliada à suplementação com farelo de trigo, para suprir uma eventual perda nutricional decorrente da desidratação do sangue.The objective of the present study was to evaluate the influence of meridic diets composed by blood from different animal species upon the adult emergence of Ctenocephalides felis felis fleas. Six artificial diets containing dried blood of cattle (standard, dogs, rabbit and chicken, combined with sand or sand/wheat bran were prepared. For each diet six samples containing ten C. f. felis eggs were evaluated. After 25 days of incubation, samples were assessed for adult emergence. The number of emerge fleas for diets composed by bovine, canine, rabbit and chicken combined with sand/wheat bran was aproximally eight fleas. Diets composed by bovine and canine combined with sand, this emergence was aproximally one flea, for both. It can be concluded that the blood origin do not

  9. Efficacy of selamectin against adult flea infestations (Ctenocephalides felis felis and Ctenocephalides canis) on dogs and cats.

    Science.gov (United States)

    McTier, T L; Jones, R L; Holbert, M S; Murphy, M G; Watson, P; Sun, F; Smith, D G; Rowan, T G; Jernigan, A D

    2000-08-23

    Selamectin was evaluated in eight controlled studies (4 in dogs, 4 in cats) to determine the efficacy of a single topical unit dose providing the recommended minimum dosage of 6mgkg(-1) against Ctenocephalides felis felis and Ctenocephalides canis fleas on dogs and against C. felis on cats. In addition, the effect of bathing on the efficacy of selamectin against C. felis was evaluated. Identical studies were performed in Beagles and domestic shorthaired cats. For each study, animals were allocated randomly to treatments of 8-12 animals each. All studies (dog studies A, B, C, and D and cat studies A, B, C, and D) evaluated the efficacy of selamectin without bathing. In addition, study C in both dogs and cats evaluated efficacy with a shampoo bath at 24h after dosing, and study D evaluated the efficacy of selamectin with water soaking at 2h after dosing or with a shampoo bath at 2-6h after dosing. Dog study B evaluated efficacy against C. canis, whereas all other studies used C. felis. In each study, selamectin was administered on day 0 as a topical dose that was applied directly to the skin in a single spot at the base of the neck in front of the scapulae. Dogs and cats were infested with approximately 100 viable unfed C. felis or C. canis on days 4, 11, 18, and 27. On days 7, 14, 21, and 30, approximately 72h after infestation, a comb count of the number of viable fleas present on each animal was made. For C. felis and C. canis for dogs and cats, compared with controls, selamectin achieved significant reductions in geometric mean adult flea comb counts of > or =98.9% on days 7, 14, and 21 in all eight studies. On day 30, the reduction for C. felis remained at or above 98.0%. This included the dogs and cats that were soaked with water or bathed with shampoo at 2, 6, or 24h after treatment. There were no significant (P>0.05) differences between the flea counts from selamectin-treated animals in these studies, regardless of bathing status. On day 30, a significant

  10. Efficacy of selamectin against adult flea infestations (Ctenocephalides felis felis and Ctenocephalides canis) on dogs and cats.

    Science.gov (United States)

    McTier, T L; Jones, R L; Holbert, M S; Murphy, M G; Watson, P; Sun, F; Smith, D G; Rowan, T G; Jernigan, A D

    2000-08-23

    Selamectin was evaluated in eight controlled studies (4 in dogs, 4 in cats) to determine the efficacy of a single topical unit dose providing the recommended minimum dosage of 6mgkg(-1) against Ctenocephalides felis felis and Ctenocephalides canis fleas on dogs and against C. felis on cats. In addition, the effect of bathing on the efficacy of selamectin against C. felis was evaluated. Identical studies were performed in Beagles and domestic shorthaired cats. For each study, animals were allocated randomly to treatments of 8-12 animals each. All studies (dog studies A, B, C, and D and cat studies A, B, C, and D) evaluated the efficacy of selamectin without bathing. In addition, study C in both dogs and cats evaluated efficacy with a shampoo bath at 24h after dosing, and study D evaluated the efficacy of selamectin with water soaking at 2h after dosing or with a shampoo bath at 2-6h after dosing. Dog study B evaluated efficacy against C. canis, whereas all other studies used C. felis. In each study, selamectin was administered on day 0 as a topical dose that was applied directly to the skin in a single spot at the base of the neck in front of the scapulae. Dogs and cats were infested with approximately 100 viable unfed C. felis or C. canis on days 4, 11, 18, and 27. On days 7, 14, 21, and 30, approximately 72h after infestation, a comb count of the number of viable fleas present on each animal was made. For C. felis and C. canis for dogs and cats, compared with controls, selamectin achieved significant reductions in geometric mean adult flea comb counts of > or =98.9% on days 7, 14, and 21 in all eight studies. On day 30, the reduction for C. felis remained at or above 98.0%. This included the dogs and cats that were soaked with water or bathed with shampoo at 2, 6, or 24h after treatment. There were no significant (P>0.05) differences between the flea counts from selamectin-treated animals in these studies, regardless of bathing status. On day 30, a significant

  11. Genomic diversification in strains of Rickettsia felis Isolated from different arthropods.

    Science.gov (United States)

    Gillespie, Joseph J; Driscoll, Timothy P; Verhoeve, Victoria I; Utsuki, Tadanobu; Husseneder, Claudia; Chouljenko, Vladimir N; Azad, Abdu F; Macaluso, Kevin R

    2015-01-01

    Rickettsia felis (Alphaproteobacteria: Rickettsiales) is the causative agent of an emerging flea-borne rickettsiosis with worldwide occurrence. Originally described from the cat flea, Ctenocephalides felis, recent reports have identified R. felis from other flea species, as well as other insects and ticks. This diverse host range for R. felis may indicate an underlying genetic variability associated with host-specific strains. Accordingly, to determine a potential genetic basis for host specialization, we sequenced the genome of R. felis str. LSU-Lb, which is an obligate mutualist of the parthenogenic booklouse Liposcelis bostrychophila (Insecta: Psocoptera). We also sequenced the genome of R. felis str. LSU, the second genome sequence for cat flea-associated strains (cf. R. felis str. URRWXCal2), which are presumably facultative parasites of fleas. Phylogenomics analysis revealed R. felis str. LSU-Lb diverged from the flea-associated strains. Unexpectedly, R. felis str. LSU was found to be divergent from R. felis str. URRWXCal2, despite sharing similar hosts. Although all three R. felis genomes contain the pRF plasmid, R. felis str. LSU-Lb carries an additional unique plasmid, pLbaR (plasmid of L. bostrychophila associated Rickettsia), nearly half of which encodes a unique 23-gene integrative conjugative element. Remarkably, pLbaR also encodes a repeats-in-toxin-like type I secretion system and associated toxin, heretofore unknown from other Rickettsiales genomes, which likely originated from lateral gene transfer with another obligate intracellular parasite of arthropods, Cardinium (Bacteroidetes). Collectively, our study reveals unexpected genomic diversity across three R. felis strains and identifies several diversifying factors that differentiate facultative parasites of fleas from obligate mutualists of booklice. PMID:25477419

  12. Molecular evidence of Rickettsia felis infection in dogs from northern territory, Australia

    Directory of Open Access Journals (Sweden)

    Rees Robert L

    2011-10-01

    Full Text Available Abstract The prevalence of spotted fever group rickettsial infection in dogs from a remote indigenous community in the Northern Territory (NT was determined using molecular tools. Blood samples collected from 130 dogs in the community of Maningrida were subjected to a spotted fever group (SFG-specific PCR targeting the ompB gene followed by a Rickettsia felis-specific PCR targeting the gltA gene of R. felis. Rickettsia felis ompB and gltA genes were amplified from the blood of 3 dogs. This study is the first report of R. felis infection in indigenous community dogs in NT.

  13. FEL beam qualities and application researches at the FELI

    International Nuclear Information System (INIS)

    The FELI four FEL facilities (FEL-1, FEL-2, FEL-3 and FEL-4) are open for users to supply several MW-level FELs covering a wide wavelength range from 278 nm to 40 μm. The ultraviolet (UV)- and visible-FEL facility (FEL-3) keeps the world record for the shortest wavelength oscillation of linac-based FELs with a thermionic gun since 1995. Technical challenges have been tried at simultaneous FEL beam sharing with fan-shaped mirrors for multistations including two manipulators and at FEL beam diagnostics of micropulse length and beam profile. The latter is for the evaluations of FEL peak power densities at irradiated spots. FEL peak power densities and adequate wavelengths are evaluated at thirteen research subjects performed at the FELI. The application researches have been performed distributed in a wide field of material science, chemical technology and bio-medical applications. (Copyright (c) 1998 Elsevier Science B.V., Amsterdam. All rights reserved.)

  14. Outbreaks of Rickettsia felis in Kenya and Senegal, 2010

    Centers for Disease Control (CDC) Podcasts

    2010-06-09

    This podcast describes the outbreak of Rickettsia felis in Kenya between August 2006 and June 2008, and in rural Senegal from November 2008 through July 2009. CDC infectious disease pathologist Dr. Chris Paddock discusses what researchers learned about this flea-borne disease and how to prevent infection.  Created: 6/9/2010 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 6/24/2010.

  15. Efecto antioxidante y antimicrobiano de sales de ácidos orgánicos y extractos naturales en filetes de bagre dorado (Brachyplatystoma rousseauxii) refrigerados

    OpenAIRE

    José Vicente Pacheco Guerrero; Elisabetta Tomé; Marisa Guerra; Rosa Raybaudi

    2011-01-01

    En el presente trabajo fueron investigados los efectos del lactato de sodio, acetato de sodio, romero y ajo en soluciones acuosas a 2,5 % sobre la calidad microbiológica y oxidación lipídica en rebanadas de bagre dorado (Brachyplatystoma rousseauxii) almacenadas a 4 ºC. Los resultados mostraron que estas soluciones fueron eficaces (p < 0,05) contra la proliferación de distintas categorías de microorganismos deteriorativos, incluyendo poblaciones aeróbicas y psicrotróficas, Pseudomonas spp., b...

  16. Modelo de restauración de áreas degradadas por minería en El Bagre – Antioquia

    OpenAIRE

    Vanegas Villa, Huber Alexander

    2014-01-01

    Resumen: Actualmente la minería aurífera aluvial, explota la mayor parte del área de humedales asociados al río Nechí en el municipio del Bagre, Antioquia - Colombia. En los humedales operados por las grandes empresas mineras, sus programas de “recuperación de tierras” en el Bajo Cauca antioqueño, incluyen medidas como reforestación, parcelas productivas, recuperación de humedales, conformación de diques de protección, manejo de sedimentos, etc. Sin embargo, se han identificado algunos efecto...

  17. Life cycle of Cystoisospora felis (Coccidia: Apicomplexa) in cats and mice

    Science.gov (United States)

    Cystoisospora felis is a ubiquitous apicomplexan protozoon of cats. The endogenous development of C. felis was studied in cats after feeding them infected mice. For this, 5 newborn cats were killed at 24, 48, 72, 96, and 120 h after having been fed mesenteric lymph nodes and spleens of mice that wer...

  18. Rickettsia felis Infection in a Common Household Insect Pest, Liposcelis bostrychophila (Psocoptera: Liposcelidae)▿ †

    Science.gov (United States)

    Behar, Adi; McCormick, Laurie J.; Perlman, Steve J.

    2010-01-01

    Many species of Rickettsia are well-known mammalian pathogens transmitted by blood-feeding arthropods. However, molecular surveys are continually uncovering novel Rickettsia species, often in unexpected hosts, including many arthropods that do not feed on blood. This study reports a systematic molecular characterization of a Rickettsia infecting the psocid Liposcelis bostrychophila (Psocoptera: Liposcelidae), a common and cosmopolitan household pest. Surprisingly, the psocid Rickettsia is shown to be Rickettsia felis, a human pathogen transmitted by fleas that causes serious morbidity and occasional mortality. The plasmid from the psocid R. felis was sequenced and was found to be virtually identical to the one in R. felis from fleas. As Liposcelis insects are often intimately associated with humans and other vertebrates, it is speculated that they acquired R. felis from fleas. Whether the R. felis in psocids causes disease in vertebrates is not known and warrants further study. PMID:20139311

  19. Variación estacional de las características seminales del bagre rayado Pseudoplatystoma metaense (Telostei, pimelodidae

    Directory of Open Access Journals (Sweden)

    Juan Ramirez-Merlano

    2011-04-01

    Full Text Available Objetivo. Determinar la variación de la calidad, el contenido iónico y la osmolaridad del plasma seminal de Pseudoplatystoma metaense durante la estación reproductiva. Materiales y métodos. Se utilizaron machos sexualmente maduros durante un periodo reproductivo (marzo a agosto. La espermiación fue inducida con Extracto de Hipófisis de Carpa (4 mg/kg. Se evaluó el volumen (mL, movilidad masal (%, tiempo de activación (sg, espermatocrito (%; concentración espermática (106 sptz/µL y viabilidad (%. También fue determinada la movilidad (% y velocidad individual por medio de un Sistema de Análisis Espermático Asistido por Computador (CASA. La osmolaridad (mOsm/Kg y la concentración de iones (Na+, Cl+, K+ y Mg2+ y glucosa fue determinada en plasma seminal obtenido por centrifugación del semen a 14.000 g. La concentración de iones y glucosa fue establecida a través de un sistema de reflectancia. Resultados. El semen de Pseudoplatystoma metaense presentó una movilidad masal mayor al 90%, con el menor valor de movilidad progresiva lineal rápida para el mes de abril (34.9±9.0% y en general valores de espermatozoides inmóviles menores al 10%. El plasma seminal mostró una osmolaridad de 259.3± 3.5 mOsm/Kg, con una concentración promedio de glucosa e iones Na+, Cl+, K+ y Mg2+ de 142.7±2.8, 118±2.2, 4.8±0.4 y 0.33±0.0 mmol/L, respectivamente, durante la época reproductiva. Conclusiones. La calidad seminal de bagre rayado no presentó variaciones en la estación reproductiva mostrando una alta calidad para los procesos de fecundación.

  20. HISTOLOGÍA Y MORFOMETRÍA DEL SISTEMA DIGESTIVO DEL SILÚRIDO BAGRE TIGRITO (Pimelodus pictus

    Directory of Open Access Journals (Sweden)

    CM Olaya

    2007-01-01

    Full Text Available El bagre tigrito Pimelodus pictus es un pez ornamental con gran aceptación en el mercado acuarista internacional, sin embargo, se desconocen muchos aspectos de la biología básica de esta especie. Por tal motivo, se realizó un estudio histológico y morfométrico del sistema digestivo a Àn de contribuir al diseño de futuras dietas. Se sacriÀcaron 7 individuos adultos de P. pictus de 10 ± 0,5 cm de longitud total con una sobredosis de MS-222 (0,5 g/l. Los especímenes se Àjaron en formaldehído al 4%, durante cinco días a 4 °C. Luego se siguió el procedimiento para técnica en paraÀna. Se realizaron cortes de 5 μm de espesor y se colo-rearon con H&E. El sistema digestivo presentó cuatro capas: mucosa, submucosa, muscular y serosa. En el esófago la mucosa estaba compuesta por epitelio plano estratiÀcado con gran número de células caliciformes, y una submucosa de tejido conectivo laxo, seguida por músculo estriado esquelético con dos orientaciones (circular y longitudinal. En todos los órganos evaluados la capa serosa fue muy delgada. En el estómago se identiÀcaron dos regiones, la pilórica y la cardíaca, encontrándose glándulas gástricas en esta última, ambas regiones presentaban una mucosa con epitelio cilíndrico simple, una submucosa de tejido conectivo laxo, y una capa de músculo liso con dos orientaciones (circular y longitudinal así mismo, este órgano fue el que exhibió el mayor espesor en la capa mucosa y muscular. La histología de la mucosa, la submucosa y la capa muscular del intestino fue similar a lo presentado por el estómago, aunque morfométricamente esta región fue la que exhibió los menores valores en todas las capas evaluadas. El estómago bien deÀnido y el intestino con pocos ciegos pilóricos hace suponer que P. pictus es de hábitos omnívoros con preferencia de los alimentos de origen animal.

  1. Clusters in social behaviour of female domestic cats (Felis silvestris catus) living in confinement

    NARCIS (Netherlands)

    vandenBos, R; de Vries, Han

    1996-01-01

    Associations between different agonistic and affiliative behavioural patterns of female domestic cats (Felis silvestris catus) were studied. In three groups of intact cats living in confinement frequencies of fourteen agonistic and affiliative behavioural patterns were recorded. The technique of fac

  2. Efecto antioxidante y antimicrobiano de sales de ácidos orgánicos y extractos naturales en filetes de bagre dorado (Brachyplatystoma rousseauxii refrigerados

    Directory of Open Access Journals (Sweden)

    José Vicente Pacheco Guerrero

    2011-06-01

    Full Text Available En el presente trabajo fueron investigados los efectos del lactato de sodio, acetato de sodio, romero y ajo en soluciones acuosas a 2,5 % sobre la calidad microbiológica y oxidación lipídica en rebanadas de bagre dorado (Brachyplatystoma rousseauxii almacenadas a 4 ºC. Los resultados mostraron que estas soluciones fueron eficaces (p acetato de sodio > lactato de sodio > ajo. La vida útil de los productos tratados fue, al menos, de 15 días. Por lo tanto, el acetato de sodio, lactato de sodio, romero y ajo pueden ser utilizados como preservativos seguros para el pescado almacenado bajo refrigeración.

  3. Efecto antioxidante y antimicrobiano de sales de ácidos orgánicos y extractos naturales en filetes de bagre dorado (Brachyplatystoma rousseauxii refrigerados

    Directory of Open Access Journals (Sweden)

    José Vicente Pacheco Guerrero

    2011-01-01

    Full Text Available En el presente trabajo fueron investigados los efectos del lactato de sodio, acetato de sodio, romero y ajo en soluciones acuosas a 2,5 % sobre la calidad microbiológica y oxidación lipídica en rebanadas de bagre dorado (Brachyplatystoma rousseauxii almacenadas a 4 ºC. Los resultados mostraron que estas soluciones fueron eficaces (p acetato de sodio > lactato de sodio > ajo. La vida útil de los productos tratados fue, al menos, de 15 días. Por lo tanto, el acetato de sodio, lactato de sodio, romero y ajo pueden ser utilizados como preservativos seguros para el pescado almacenado bajo refrigeración.

  4. Biomarcadores histológicos em duas espécies de bagres estuarinos da Costa Maranhense, Brasil Histology biomarkers in two estuarine catfish species from the Maranhense Coast, Brazil

    Directory of Open Access Journals (Sweden)

    D.B.P.Sousa

    2013-04-01

    Full Text Available Neste estudo objetivou-se validar lesões branquiais como biomarcadores em peixes de importância econômica (Sciades herzbergii e Bagre bagre capturados pela pesca artesanal em dois locais diferenciados da Baía de São Marcos, MA, a fim de selecionar um táxon como bioindicador capaz de ser utilizado em programas de biomonitoramento. O primeiro ponto (S1 foi utilizado como uma área de referência - Ilha dos Caranguejos -, e o segundo ponto (S2 foi considerado potencialmente impactado - Complexo Portuário de São Luís, MA. Encontraram-se várias alterações histológicas para as duas espécies em S2, destacando-se: estreitamento lamelar, teleangectasia, fusão e descolamento do epitélio da lamela secundária. Lesões branquiais em B. bagre coletados em S1 também foram observadas, não sendo possível diferenciar os indivíduos das duas áreas analisadas. Os dados indicam que S. herzbergii é um bioindicador mais apropriado para análise de biomarcadores de contaminação aquática.This study aimed to validate branchial lesions as a biomarker in fish (Sciades herzbergii and Bagre bagre caught by traditional fishing in two different places at São Marcos Bay, MA, in order to select a bioindicator taxon which can be used in biomonitoring programs. The first point (S1 was used as a reference area - Caranguejos (Crab Island and the second point (S2 was considered potentially impacted - Port Complex of São Luis, MA. Several histological changes were found for both species in S2, including: narrowing lamellar, teleangectasy, fusion and separation of secondary lamellar epithelium. Gill lesions in B. bagre collected were also observed in S1, it is not possible to differentiate between individuals of the two areas analyzed. The data indicate that S. herzbergii is more appropriate as a bioindicator for analysis of biomarkers of aquatic contamination.

  5. Chlamydia felis exposure in companion dogs and cats in Lanzhou, China: a public health concern

    OpenAIRE

    Wu, Song-Ming; Huang, Si-Yang; Xu, Min-Jun; Zhou, Dong-Hui; Song, Hui-Qun; Zhu, Xing-Quan

    2013-01-01

    Background Chlamydiaceae is a family of obligate intracellular pathogens with a worldwide distribution in many animal species, including humans. No information exists on the prevalence of Chlamydia felis infections in cats and dogs in Lanzhou, the geographical center of China. The aim of this study was to carry out a census of cats and dogs in Lanzhou and document the seroprevalence of C. felis exposure in these companion animals. Results In this study, blood samples were collected from 485 a...

  6. Plasmids and rickettsial evolution: insight from Rickettsia felis.

    Directory of Open Access Journals (Sweden)

    Joseph J Gillespie

    Full Text Available BACKGROUND: The genome sequence of Rickettsia felis revealed a number of rickettsial genetic anomalies that likely contribute not only to a large genome size relative to other rickettsiae, but also to phenotypic oddities that have confounded the categorization of R. felis as either typhus group (TG or spotted fever group (SFG rickettsiae. Most intriguing was the first report from rickettsiae of a conjugative plasmid (pRF that contains 68 putative open reading frames, several of which are predicted to encode proteins with high similarity to conjugative machinery in other plasmid-containing bacteria. METHODOLOGY/PRINCIPAL FINDINGS: Using phylogeny estimation, we determined the mode of inheritance of pRF genes relative to conserved rickettsial chromosomal genes. Phylogenies of chromosomal genes were in agreement with other published rickettsial trees. However, phylogenies including pRF genes yielded different topologies and suggest a close relationship between pRF and ancestral group (AG rickettsiae, including the recently completed genome of R. bellii str. RML369-C. This relatedness is further supported by the distribution of pRF genes across other rickettsiae, as 10 pRF genes (or inactive derivatives also occur in AG (but not SFG rickettsiae, with five of these genes characteristic of typical plasmids. Detailed characterization of pRF genes resulted in two novel findings: the identification of oriV and replication termination regions, and the likelihood that a second proposed plasmid, pRFdelta, is an artifact of the original genome assembly. CONCLUSION/SIGNIFICANCE: Altogether, we propose a new rickettsial classification scheme with the addition of a fourth lineage, transitional group (TRG rickettsiae, that is unique from TG and SFG rickettsiae and harbors genes from possible exchanges with AG rickettsiae via conjugation. We offer insight into the evolution of a plastic plasmid system in rickettsiae, including the role plasmids may have played in

  7. Three-dimensional morphology, ultrastructure, and replication of Mycoplasma felis.

    Science.gov (United States)

    Boatman, E S; Kenny, G E

    1970-01-01

    The morphology and replication of Mycoplasma felis in relation to growth phase in culture were studied by electron microscopy. The organisms showed 1.0 to 1.45-hr doubling times with typical bacterial-type growth curves when grown in dialysate broth supplemented with horse serum. Organisms were fixed for electron microscopy by using Veronal acetate-buffered 0.8% OsO(4) (pH 6.1) in 20% sucrose. The morphology of exponential-phase organisms differed markedly from that of stationary or death-phase organisms, which were essentially large round forms with either dispersed or abnormally aggregated cytoplasm. Plasticine models prepared from serial sections of organisms in exponential phase showed the organisms to be either disc-shaped, triangular, horseshoe-shaped, or multilobular. A central "hole" was frequently present in these structures and could be visualized in the lobular forms as an interconnecting circular membrane. The inner surface of this membrane often showed contact with a small membranous body about 0.12 mum in diameter. The significance of this body is unknown. The morphology of the various shapes was confirmed by using the phosphotungstic acid and critical point methods. When the ratios of the various forms in exponential-phase cultures were determined, it was found that a replication sequence could be proposed which accounted for not only the volume increase required to accommodate deoxyribonucleic acid (DNA) replication but also the distribution of that DNA. Although it is likely that DNA replication in M. felis is a binary process, it appears that the mechanism for production of new cells need not be a binary process. PMID:5411752

  8. Molecular and morphologic characterization of Sarcocystis felis (Apicomplexa: Sarcocystidae) in South American wild felids from Brazil.

    Science.gov (United States)

    Cañón-Franco, William Alberto; López-Orozco, Natalia; Christoff, Alexandre Uarth; de Castilho, Camila Schlieper; de Araújo, Flavio Antônio Pacheco; Verma, Shiv Kumar; Dubey, J P; Soares, Rodrigo Martins; Gennari, Solange Maria

    2016-02-15

    Wild felids are thought to share parasites with domestic cats. However, little is known of the coccidian parasites of wild felids. We investigated the presence of Sarcocystis spp. in tissues of 6 species of 90 Neotropical small felids killed in road accidents in the state of Rio Grande do Sul, Brazil by using microscopic and molecular techniques. Formalin-fixed tissues from 28 felids were examined, and Sarcocystis felis-like sarcocysts were detected in 4 wild cats (2 Puma yagouaroundi and 2 Leopardus guttulus). By transmission electron microscopy, sarcocysts from a P. yagouaroundi were identical to S. felis from domestic cats in the USA. Direct sequencing of PCR amplicons resulted the unambiguous sequences of the ITS-1 region from 18 of the 31 PCR positive wild cats; 5 sequences from each P. yagouaroundi, and Leopardus geoffroyi, 4 sequences from L. guttulus, and 2 sequences from each Leopardus wiedii, and Leopardus colocolo. Sequences analysis of ITS-1 region revealed the highest identiy (97-99%) with that of previously describe isolates of S. felis from domestic cats in the USA and identified them as S. felis. Tissues of 1 Leopardus pardalis tested by PCR and histology were negative. The phylogenetic relationship indicated that S. felis is quite different to species which employ opossums as their definitive host. This is the first report of S. felis infection in small wild felids from Brazil. PMID:26827854

  9. Molecular evidence for the presence of Rickettsia Felis in the feces of wild-living African apes.

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    Alpha Kabinet Keita

    Full Text Available BACKGROUND: Rickettsia felis is a common emerging pathogen detected in mosquitoes in sub-Saharan Africa. We hypothesized that, as with malaria, great apes may be exposed to the infectious bite of infected mosquitoes and release R. felis DNA in their feces. METHODS: We conducted a study of 17 forest sites in Central Africa, testing 1,028 fecal samples from 313 chimpanzees, 430 gorillas and 285 bonobos. The presence of rickettsial DNA was investigated by specific quantitative real-time PCR. Positive results were confirmed by a second PCR using primers and a probe targeting a specific gene for R. felis. All positive samples were sequenced. RESULTS: Overall, 113 samples (11% were positive for the Rickettsia-specific gltA gene, including 25 (22% that were positive for R. felis. The citrate synthase (gltA sequence and outer membrane protein A (ompA sequence analysis indicated 99% identity at the nucleotide level to R. felis. The 88 other samples (78% were negative using R. felis-specific qPCR and were compatible with R. felis-like organisms. CONCLUSION: For the first time, we detected R. felis in wild-living ape feces. This non invasive detection of human pathogens in endangered species opens up new possibilities in the molecular epidemiology and evolutionary analysis of infectious diseases, beside HIV and malaria.

  10. Studying Cat (Felis catus Diabetes: Beware of the Acromegalic Imposter.

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    Stijn J M Niessen

    Full Text Available Naturally occurring diabetes mellitus (DM is common in domestic cats (Felis catus. It has been proposed as a model for human Type 2 DM given many shared features. Small case studies demonstrate feline DM also occurs as a result of insulin resistance due to a somatotrophinoma. The current study estimates the prevalence of hypersomatotropism or acromegaly in the largest cohort of diabetic cats to date, evaluates clinical presentation and ease of recognition. Diabetic cats were screened for hypersomatotropism using serum total insulin-like growth factor-1 (IGF-1; radioimmunoassay, followed by further evaluation of a subset of cases with suggestive IGF-1 (>1000 ng/ml through pituitary imaging and/ or histopathology. Clinicians indicated pre-test suspicion for hypersomatotropism. In total 1221 diabetic cats were screened; 319 (26.1% demonstrated a serum IGF-1>1000 ng/ml (95% confidence interval: 23.6-28.6%. Of these cats a subset of 63 (20% underwent pituitary imaging and 56/63 (89% had a pituitary tumour on computed tomography; an additional three on magnetic resonance imaging and one on necropsy. These data suggest a positive predictive value of serum IGF-1 for hypersomatotropism of 95% (95% confidence interval: 90-100%, thus suggesting the overall hypersomatotropism prevalence among UK diabetic cats to be 24.8% (95% confidence interval: 21.2-28.6%. Only 24% of clinicians indicated a strong pre-test suspicion; most hypersomatotropism cats did not display typical phenotypical acromegaly signs. The current data suggest hypersomatotropism screening should be considered when studying diabetic cats and opportunities exist for comparative acromegaly research, especially in light of the many detected communalities with the human disease.

  11. Amyloidosis in black-footed cats (Felis nigripes).

    Science.gov (United States)

    Terio, K A; O'Brien, T; Lamberski, N; Famula, T R; Munson, L

    2008-05-01

    A high prevalence of systemic amyloidosis was documented in the black-footed cat (Felis nigripes) based on a retrospective review of necropsy tissues (n = 38) submitted as part of ongoing disease surveillance. Some degree of amyloid deposition was present in 33 of 38 (87%) of the examined cats, and amyloidosis was the most common cause of death (26/38, 68%). Amyloid deposition was most severe in the renal medullary interstitium (30/33, 91%) and glomeruli (21/33, 63%). Other common sites included the splenic follicular germinal centers (26/31, 84%), gastric lamina propria (9/23, 39%), and intestinal lamina propria (3/23, 13%). Amyloid in all sites stained with Congo red, and in 13 of 15 (87%) cats, deposits had strong immunoreactivity for canine AA protein by immunohistochemistry. There was no association with concurrent chronic inflammatory conditions (P = .51), suggesting that amyloidosis was not secondary to inflammation. Adrenal cortical hyperplasia, a morphologic indicator of stress that can predispose to amyloid deposition, was similarly not associated (P = .09) with amyloidosis. However, adrenals were not available from the majority of cats without amyloidosis; therefore, further analysis of this risk factor is warranted. Heritability estimation suggested that amyloidosis might be familial in this species. Additionally, tissues from a single free-ranging black-footed cat had small amounts of amyloid deposition, suggesting that there could be a predilection for amyloidosis in this species. Research to identify the protein sequence of serum amyloid A (SAA) in the black-footed cat is needed to further investigate the possibility of an amyloidogenic SAA in this species.

  12. Rickettsial Infections among Ctenocephalides felis and Host Animals during a Flea-Borne Rickettsioses Outbreak in Orange County, California

    Science.gov (United States)

    Fogarty, Carrie; Krueger, Laura; Macaluso, Kevin R.; Odhiambo, Antony; Nguyen, Kiet; Farris, Christina M.; Luce-Fedrow, Alison; Bennett, Stephen; Jiang, Ju; Sun, Sokanary; Cummings, Robert F.; Richards, Allen L.

    2016-01-01

    Due to a resurgence of flea-borne rickettsioses in Orange County, California, we investigated the etiologies of rickettsial infections of Ctenocephalides felis, the predominant fleas species obtained from opossums (Didelphis virginiana) and domestic cats (Felis catus), collected from case exposure sites and other areas in Orange County. In addition, we assessed the prevalence of IgG antibodies against spotted fever group (SFGR) and typhus group (TGR) rickettsiae in opossum sera. Of the 597 flea specimens collected from opossums and cats, 37.2% tested positive for Rickettsia. PCR and sequencing of rickettsial genes obtained from C. felis flea DNA preparations revealed the presence of R. typhi (1.3%), R. felis (28.0%) and R. felis-like organisms (7.5%). Sera from opossums contained TGR-specific (40.84%), but not SFGR-specific antibodies. The detection of R. felis and R. typhi in the C. felis fleas in Orange County highlights the potential risk for human infection with either of these pathogens, and underscores the need for further investigations incorporating specimens from humans, animal hosts, and invertebrate vectors in endemic areas. Such studies will be essential for establishing a link in the ongoing flea-borne rickettsioses outbreaks. PMID:27537367

  13. INFECTION BY Rickettsia felis IN OPOSSUMS (Didelphis sp. FROM YUCATAN, MEXICO

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    Gaspar PENICHE-LARA

    2016-01-01

    Full Text Available Rickettsia felis is an emergent pathogen and the causative agent of a typhus-like rickettsiosis in the Americas. Its transmission cycle involves fleas as biological vectors (mainly Ctenocephalides felis and multiple domestic and synanthropic mammal hosts. Nonetheless, the role of mammals in the cycle of R. felis is not well understood and many efforts are ongoing in different countries of America to clarify it. The present study describes for the first time in Mexico the infection of two species of opossum (Didelphis virginiana and D. marsupialis by R. felis. A diagnosis was carried out from blood samples by molecular methods through the gltAand 17 kDa genes and sequence determination. Eighty-seven opossum samples were analyzed and 28 were found to be infected (32.1% from five out of the six studied localities of Yucatan. These findings enable recognition of the potential epidemiological implications for public health of the presence of infected synanthropic Didelphis in households.

  14. INFECTION BY Rickettsia felis IN OPOSSUMS (Didelphis sp.) FROM YUCATAN, MEXICO.

    Science.gov (United States)

    Peniche-Lara, Gaspar; Ruiz-Piña, Hugo A; Reyes-Novelo, Enrique; Dzul-Rosado, Karla; Zavala-Castro, Jorge

    2016-01-01

    Rickettsia felis is an emergent pathogen and the causative agent of a typhus-like rickettsiosis in the Americas. Its transmission cycle involves fleas as biological vectors (mainly Ctenocephalides felis) and multiple domestic and synanthropic mammal hosts. Nonetheless, the role of mammals in the cycle of R. felis is not well understood and many efforts are ongoing in different countries of America to clarify it. The present study describes for the first time in Mexico the infection of two species of opossum (Didelphis virginiana and D. marsupialis) by R. felis. A diagnosis was carried out from blood samples by molecular methods through the gltA and 17 kDa genes and sequence determination. Eighty-seven opossum samples were analyzed and 28 were found to be infected (32.1%) from five out of the six studied localities of Yucatan. These findings enable recognition of the potential epidemiological implications for public health of the presence of infected synanthropic Didelphis in households. PMID:27074326

  15. INFECTION BY Rickettsia felis IN OPOSSUMS (Didelphis sp.) FROM YUCATAN, MEXICO

    Science.gov (United States)

    PENICHE-LARA, Gaspar; RUIZ-PIÑA, Hugo A.; REYES-NOVELO, Enrique; DZUL-ROSADO, Karla; ZAVALA-CASTRO, Jorge

    2016-01-01

    Rickettsia felis is an emergent pathogen and the causative agent of a typhus-like rickettsiosis in the Americas. Its transmission cycle involves fleas as biological vectors (mainly Ctenocephalides felis) and multiple domestic and synanthropic mammal hosts. Nonetheless, the role of mammals in the cycle of R. felis is not well understood and many efforts are ongoing in different countries of America to clarify it. The present study describes for the first time in Mexico the infection of two species of opossum (Didelphis virginiana and D. marsupialis) by R. felis. A diagnosis was carried out from blood samples by molecular methods through the gltAand 17 kDa genes and sequence determination. Eighty-seven opossum samples were analyzed and 28 were found to be infected (32.1%) from five out of the six studied localities of Yucatan. These findings enable recognition of the potential epidemiological implications for public health of the presence of infected synanthropic Didelphis in households. PMID:27074326

  16. Possible Role of Rickettsia felis in Acute Febrile Illness among Children in Gabon.

    Science.gov (United States)

    Mourembou, Gaël; Lekana-Douki, Jean Bernard; Mediannikov, Oleg; Nzondo, Sydney Maghendji; Kouna, Lady Charlene; Essone, Jean Claude Biteghe Bi; Fenollar, Florence; Raoult, Didier

    2015-10-01

    Rickettsia felis has been reported to be a cause of fever in sub-Saharan Africa, but this association has been poorly evaluated in Gabon. We assessed the prevalence of this bacterium among children Gabon; the locations were in urban, semiurban, and rural areas. DNA samples from 410 febrile children and 60 afebrile children were analyzed by quantitative PCR. Overall, the prevalence of R. felis among febrile and afebrile children was 10.2% (42/410 children) and 3.3% (2/60 children), respectively. Prevalence differed among febrile children living in areas that are urban (Franceville, 1.3% [1/77]), semiurban (Koulamoutou, 2.1% [3/141]), and rural (Lastourville, 11.2% [15/134]; Fougamou, 39.7% [23/58]). Furthermore, in a rural area (Fougamou), R. felis was significantly more prevalent in febrile (39.7% [23/58]) than afebrile children (5.0% [1/20]). Additional studies are needed to better understand the pathogenic role of R. felis in this part of the world.

  17. Anti-inflammatory and Anti-tumorigenic Effects of Açai Berry in Helicobacter felis-infected mice

    OpenAIRE

    Lee, Ju Yup; Kim, Nayoung; Choi, Yoon Jeong; Nam, Ryoung Hee; Lee, Seonmin; Ham, Min Hee; Suh, Ji Hyung; Choi, Yoon Jin; Lee, Hye Seung; Lee, Dong Ho

    2016-01-01

    Background: The aim of this study was to evaluate the anti-inflammatory and anti-tumorigenic effect of açai berry after chronic Helicobacter felis colonization in the stomachs of C57BL/6 mice. Methods: A total of 57 four-week-old female C57BL/6 mice (18 control mice and 39 experimental mice) were used. The mice were administered orogastrically with vehicle only or vehicle containing H. felis, 5 times every other day. After inoculation of H. felis, mice were fed either a standard or an açai-co...

  18. Molecular detection of Rickettsia felis and Candidatus Rickettsia asemboensis in fleas from human habitats, Asembo, Kenya.

    Science.gov (United States)

    Jiang, Ju; Maina, Alice N; Knobel, Darryn L; Cleaveland, Sarah; Laudisoit, Anne; Wamburu, Kabura; Ogola, Eric; Parola, Philippe; Breiman, Robert F; Njenga, M Kariuki; Richards, Allen L

    2013-08-01

    The flea-borne rickettsioses murine typhus (Rickettsia typhi) and flea-borne spotted fever (FBSF) (Rickettsia felis) are febrile diseases distributed among humans worldwide. Murine typhus has been known to be endemic to Kenya since the 1950s, but FBSF was only recently documented in northeastern (2010) and western (2012) Kenya. To characterize the potential exposure of humans in Kenya to flea-borne rickettsioses, a total of 330 fleas (134 pools) including 5 species (Xenopsylla cheopis, Ctenocephalides felis, Ctenocephalides canis, Pulex irritans, and Echidnophaga gallinacea) were collected from domestic and peridomestic animals and from human dwellings within Asembo, western Kenya. DNA was extracted from the 134 pooled flea samples and 89 (66.4%) pools tested positively for rickettsial DNA by 2 genus-specific quantitative real-time PCR (qPCR) assays based upon the citrate synthase (gltA) and 17-kD antigen genes and the Rfelis qPCR assay. Sequences from the 17-kD antigen gene, the outer membrane protein (omp)B, and 2 R. felis plasmid genes (pRF and pRFd) of 12 selected rickettsia-positive samples revealed a unique Rickettsia sp. (n=11) and R. felis (n=1). Depiction of the new rickettsia by multilocus sequence typing (MLST) targeting the 16S rRNA (rrs), 17-kD antigen gene, gltA, ompA, ompB, and surface cell antigen 4 (sca4), shows that it is most closely related to R. felis but genetically dissimilar enough to be considered a separate species provisionally named Candidatus Rickettsia asemboensis. Subsequently, 81 of the 134 (60.4%) flea pools tested positively for Candidatus Rickettsia asemboensis by a newly developed agent-specific qPCR assay, Rasemb. R. felis was identified in 9 of the 134 (6.7%) flea pools, and R. typhi the causative agent of murine typhus was not detected in any of 78 rickettsia-positive pools assessed using a species-specific qPCR assay, Rtyph. Two pools were found to contain both R. felis and Candidatus Rickettsia asemboensis DNA and 1 pool

  19. Conservation inequality and the charismatic cat: Felis felicis

    Directory of Open Access Journals (Sweden)

    E.A. Macdonald

    2015-01-01

    . While the felids are widely regarded as a popular taxonomic group, the great extent to which they appealed to our respondents emphasises their potential as ambassadors for conservation. Indeed, the big cats were so highly rated that we might think of them as one, Felis felicis: a globally powerful flagship for conservation.

  20. Bases científicas para contribuir a la gestión de la pesquería comercial de bagres (familia pimelodidae) en la Amazonia colombiana y sus zonas de frontera

    OpenAIRE

    Agudelo Córdoba, Edwin

    2015-01-01

    Los peces son uno de los principales recursos alimenticios, culturales y económicos en la Amazonia, importantes para la seguridad alimentaria de los núcleos familiares ribereños como en la generación de ingreso a quienes dependen económicamente de la extracción de este recurso natural. Esta tesis doctoral, se ha enfocado en analizar variables biológicas y pesqueras en varios bagres de la familia Pimelodidae, así como el componente humano y económico de esta actividad en la frontera en Colomb...

  1. Biología reproductiva del bagre cominate Occidentarius platypogon (Pisces: Ariidae) en el sureste del golfo de California Reproductive biology of the cominate sea catfish Occidentarius platypogon (Pisces: Ariidae) from the southeastern Gulf of California

    OpenAIRE

    Felipe Amezcua; Víctor Muro-Torres

    2012-01-01

    En el sureste del golfo de California, el bagre cominate presenta gran abundancia y es explotado comercialmente. Sin embargo, estudios sobre su biología son escasos y no existen normas que regulen su explotación en México. En este trabajo se describe la biología reproductiva de Occidentarius platypogon. Se capturaron 480 organismos con redes agalleras entre octubre 2008 y diciembre 2009. El estado de madurez gonadal se determinó macroscópicamente. Esta especie tiene una época de desove de may...

  2. Clinical and laboratorial evidence of Rickettsia felis infections in Latin America Evidência clínica e laboratorial de infecções por Rickettsia felis na América Latina

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    Márcio Antônio Moreira Galvão

    2004-06-01

    Full Text Available After the discovery and initial characterization of Rickettsia felis in 1992 by Azad and cols, and the subsequent first description of a human case of infection in 1994, there have been two communications of human rickettsiosis cases caused by Rickettsia felis in Latin America. The first one was published in 2000 by Zavala-Velazquez and cols in Mexico. In 2001 Raoult and cols described the occurrence of two human cases of Rickettsia felis rickettsiosis in Brazil. In the present discussion these two articles were compared and after the description of the principal signs and symptoms, it was concluded that more studies are needed with descriptions of a greater number of patients to establish the true frequency of the clinical signs and symptoms present in Rickettsia felis rickettsiosis.Depois da descoberta e caracterização inicial da Rickettsia felis em 1992 por Azad e cols, e à descrição subseqüente do primeiro caso de infecção humana em 1994, houveram duas comunicações de rickettsioses causadas por Rickettsia felis na América Latina. A primeira foi feita por Zavala-Velazquez e cols em 2000 no México. Em 2001, Raoult e cols descreveram a ocorrência de dois casos humanos de rickettsiose por Rickettsia felis no Brasil. Na presente discussão, esses dois artigos foram comparados, e depois da descrição dos principais sinais e sintomas, conclui-se que outros estudos são necessários, com a participação de um maior número de pacientes, para se estabelecer a verdadeira freqüência dos sinais clínicos e sintomas presentes nas rickettsioses por Rickettsia felis.

  3. A new variant of endemic pemphigus foliaceus in El-Bagre, Colombia: the Hardy-Weinberg-Castle law and linked short tandem repeats

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    Ana María Abreu Velez

    2009-01-01

    Full Text Available Background : We reported a new variant of endemic pemphigus foliaceus in El Bagre, Colombia. Aims : Our study performed Complex Segregation Analysis (CSA and short tandem repeats to discriminate between environmental and/or genetic factors in this disorder. Materials and Methods: The CSA analysis was carried out according to the unified model, implemented using the transmission probabilities implemented in the computer program POINTER, and evaluated by using a software package for population genetic data analysis (GDA, Arlequin. We performed pedigree analyses by using Cyrillic 2.1 software, with a total of 30 families with 50 probands (47 males and 3 females tested. In parallel to the CSA, we tested for the presence of short tandem repeats from HLA class II, DQ alpha 1, involving the gene locus D6S291 by using the Hardy-Weinberg- Castle law. Results : Our results indicate that the best model of inheritance in this disease is a mixed model, with multifactorial effects within a recessive genotype. Two types of possible segregation patterns were found; one with strong recessive penetrance in families whose phenotype is more Amerindian-like, and another of possible somatic mutations. Conclusion : The penetrance of 10% or less in female patients 60 years of age or older indicates that hormones could protect younger females. The greatest risk factor for men being affected by the disorder was the NN genotype. These findings are only possible due to somatic mutations, and/or strong environmental effects. We also found a protective role for two genetic loci (D6S1019 AND D6S439 in the control group.

  4. Environmental changes and microbiological health risks. Satellite-derived turbidity: an indicator of "health hazard" for surface water in West Africa (Bagre lake, Burkina Faso).

    Science.gov (United States)

    Robert, E.; Grippa, M.; Kergoat, L.; Martinez, J.; Pinet, S.; Gal, L.; Soumaguel, N.

    2015-12-01

    A significant correlation exists between the concentration of parasites, bacteria and some water quality parameters including surface suspended solids (SSS) and turbidity. Suspended particles can carry viruses and pathogenic bacteria affecting human health and foster their development. High SSS, associated with high turbidity, can therefore be considered as a vector of microbiological contaminants, causing diarrheal diseases. Few studies have focused on the turbidity parameter in rural Africa, while many cases of intestinal parasitic infections are due to the consumption of unsafe water from ponds, lakes, and rivers. Monitoring turbidity may therefore contribute to health hazard monitoring. Turbidity refers to the optical properties of water and is known to impact water reflectance in the visible and near-infrared domain. Ideally, its spatial and temporal variability requires the use of high temporal resolution (MODIS) and spatial resolution (Landsat, SPOT, Sentinel-2). Here we investigate turbidity in West-Africa. Various algorithms and indices proposed in the literature for inland waters are applied to MODIS series and to Landsat 7 and 8 CDR images, and SPOT5 images. The data and algorithms are evaluated with field measurements: turbidity, SSS, and hyperspectral ground radiometry. We show that turbidity of the Bagre Lake displays a strong increase over 2000-2015, associated with the corresponding increase of the red and NIR reflectances, as well as a reduction of the seasonal variations. Water level derived from the Jason 2 altimeter does not explain such variations. The most probable hypothesis is a change in land use (increase in bare and degraded soils), that leads to an increase in the particles transported by surface runoff to the lake. Such an increase in turbidity reinforces the health risk. We will discuss the link between turbidity and health in view of data from health centers on diarrheal diseases as well as data on practices and uses of populations.

  5. Atrophic gastric changes in both Helicobacter felis and Helicobacter pylori infected mice are host dependent and separate from antral gastritis.

    OpenAIRE

    Sakagami, T; Dixon, M; O'Rourke, J; Howlett, R.; Alderuccio, F; Vella, J; Shimoyama, T; Lee, A.

    1996-01-01

    BACKGROUND/AIMS: The role of host factors has been neglected in studies of the pathogenesis of Helicobacter associated disease. The aim of this study was to assess the response of different mouse strains to infection with a single strain of Helicobacter felis. METHOD: Six strains of inbred mice were infected with the identical H felis culture and were killed at one month, two months, and six months after infection to assess histopathological changes. In addition, two strains of mice were infe...

  6. Desenvolvimento dos fungos Metarhizium anisopliae (Metschnikoff, 1879 Sorokin, 1883 E Beauveria bassiana (Balsamo Vuillemin, 1912 sobre Ctenocephalides felis felis (Bouché, 1835 Development of the fungi Metarhizium anisopliae (Metschnikoff, 1879 Sorokin, 1883 and Beauveria bassiana (Balsamo Vuillemin, 1912 on the Ctenophephalides felis felis (Bouché, 1835

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    Denise R. De Melo

    2007-09-01

    Full Text Available A pulga Ctenocephalides felis felis é um parasita causador dermatites alérgicas e também pode transmitir diversos agentes etiológicos aos animais domésticos e aos homens. O objetivo deste trabalho foi verificar o desenvolvimento do fungo sobre a cutícula da pulga, através da microscopia eletrônica de varredura. Os isolados fúngicos testados foram o Metarhizium anisopliae 959 e Beauveria bassiana 986, ambos na concentração 10(8 conídios/ml. Após a exposição dos isolados fúngicos no período de duas, 15, 26 e 96 horas , o material foi processado para a microscopia eletrônica de varredura. Com a obtenção das micrografias, pode-se observar que com 2 horas após exposição aos fungos, os conídios estavam aderidos por toda a cutícula, situando-se preferencialmente nas membranas intersegmentais do abdome. Com 15 horas observou-se a formação do tubo de germinação e a cabeça do apressório e após 26 horas foi possível observar as ramificações e o engrossamento das hifas sobre a cutícula das pulgas. Os resultados indicam que os fungos testados foram capazes de se desenvolver sobre a cutícula de C. f. felis.The flea Ctenocephalides felis felis is a parasite that causes allergic dermatitis and also may transmit etiologic agents to domestic animals and humans. This study investigated by scanning electron microscopy the development of entomopathogenic fungi on flea cuticle. Fleas were exposed to conidia (10(8 ml-1 of Metarhizium anisopliae (isolate 959 or Beauveria bassiana (isolate 986. Following standard protocols for electron microscopy, the specimens were prepared 2, 15, 26 and 96 h after infection. The micrography revealed that 2 h after fungus exposure, conidia attachments encompassed the entire flea cuticle, especially on abdominal intersegmental membranes. The emergence of germ tubes and appressoria formation occurred at 15 h, thickening and branching of hyphae on the flea cuticle was noted at 26 h. Therefore, both of

  7. The genetic integrity of the ex situ population of the European wildcat (Felis silvestris silvestris) is seriously threatened by introgression from domestic cats (Felis silvestris catus).

    Science.gov (United States)

    Witzenberger, Kathrin A; Hochkirch, Axel

    2014-01-01

    Studies on the genetic diversity and relatedness of zoo populations are crucial for implementing successful breeding programmes. The European wildcat, Felis s. silvestris, is subject to intensive conservation measures, including captive breeding and reintroduction. We here present the first systematic genetic analysis of the captive population of Felis s. silvestris in comparison with a natural wild population. We used microsatellites and mtDNA sequencing to assess genetic diversity, structure and integrity of the ex situ population. Our results show that the ex situ population of the European wildcat is highly structured and that it has a higher genetic diversity than the studied wild population. Some genetic clusters matched the breeding lines of certain zoos or groups of zoos that often exchanged individuals. Two mitochondrial haplotype groups were detected in the in situ populations, one of which was closely related to the most common haplotype found in domestic cats, suggesting past introgression in the wild. Although native haplotypes were also found in the captive population, the majority (68%) of captive individuals shared a common mtDNA haplotype with the domestic cat (Felis s. catus). Only six captive individuals (7.7%) were assigned as wildcats in the STRUCTURE analysis (at K = 2), two of which had domestic cat mtDNA haplotypes and only two captive individuals were assigned as purebred wildcats by NewHybrids. These results suggest that the high genetic diversity of the captive population has been caused by admixture with domestic cats. Therefore, the captive population cannot be recommended for further breeding and reintroduction. PMID:25162450

  8. The genetic integrity of the ex situ population of the European wildcat (Felis silvestris silvestris) is seriously threatened by introgression from domestic cats (Felis silvestris catus).

    Science.gov (United States)

    Witzenberger, Kathrin A; Hochkirch, Axel

    2014-01-01

    Studies on the genetic diversity and relatedness of zoo populations are crucial for implementing successful breeding programmes. The European wildcat, Felis s. silvestris, is subject to intensive conservation measures, including captive breeding and reintroduction. We here present the first systematic genetic analysis of the captive population of Felis s. silvestris in comparison with a natural wild population. We used microsatellites and mtDNA sequencing to assess genetic diversity, structure and integrity of the ex situ population. Our results show that the ex situ population of the European wildcat is highly structured and that it has a higher genetic diversity than the studied wild population. Some genetic clusters matched the breeding lines of certain zoos or groups of zoos that often exchanged individuals. Two mitochondrial haplotype groups were detected in the in situ populations, one of which was closely related to the most common haplotype found in domestic cats, suggesting past introgression in the wild. Although native haplotypes were also found in the captive population, the majority (68%) of captive individuals shared a common mtDNA haplotype with the domestic cat (Felis s. catus). Only six captive individuals (7.7%) were assigned as wildcats in the STRUCTURE analysis (at K = 2), two of which had domestic cat mtDNA haplotypes and only two captive individuals were assigned as purebred wildcats by NewHybrids. These results suggest that the high genetic diversity of the captive population has been caused by admixture with domestic cats. Therefore, the captive population cannot be recommended for further breeding and reintroduction.

  9. Progress in research of Rickettsia felis%猫立克次体研究进展

    Institute of Scientific and Technical Information of China (English)

    刘杨; 杨雨; 陈萍; 赵锋; 钟玮

    2015-01-01

    猫立克次体(Rickettsia felis)可以引起蚤传斑点热(Flea-Borne Spotted Fever,FBSF),主要传播媒介是猫蚤(Ctenocephalides felis).自上世纪90年代首次发现以来,已被证实分布于地球上除南极以外的各大洲.本文对猫立克次体的传播媒介、检测方法以及基因组学研究进展进行了综述,并讨论了在全球气候变化的背景下,开展猫立克次体研究的重要意义.

  10. Isolation of Rickettsia felis in the Mosquito Cell Line C6/36

    OpenAIRE

    Horta, Maurício C.; Labruna, Marcelo B.; Edison L. Durigon; Teresinha T.S. Schumaker

    2006-01-01

    We report the isolation and establishment of Rickettsia felis in the C6/36 cell line. Rickettsial growth was intense, always with 90 to 100% of cells being infected after few weeks. The rickettsial isolate was confirmed by testing infected cells by PCR and sequencing fragments of three major Rickettsia genes (gltA, ompB, and the 17-kDa protein gene).

  11. Clusters in social behaviour of female domestic cats (Felis silvestris catus) living in confinement

    OpenAIRE

    vandenBos, R; de Vries, Han

    1996-01-01

    Associations between different agonistic and affiliative behavioural patterns of female domestic cats (Felis silvestris catus) were studied. In three groups of intact cats living in confinement frequencies of fourteen agonistic and affiliative behavioural patterns were recorded. The technique of factor analysis (Principal Components Analysis followed by varimax rotation on a dyads X behavioural patterns matrix) was used to detect clusters in these behavioural patterns. Five factors (or types ...

  12. Detection of Helicobacter felis in a cat with gastric disease in laboratory animal facility

    Science.gov (United States)

    Hong, Sunhwa; Chung, Yungho; Kang, Won-Guk

    2016-01-01

    A 3-month-old male cat in the animal facility was presented for investigation of anorexia and occasional vomiting. We collected the specimens from gastroscopic biopsy and stool collection. The gastroscopic biopsy specimens were tested using a rapid urease test, CLO Helicobacter-detection kits. Stool specimens were gathered and evaluated using the commercially available SD Bioline H. pylori Ag kit according to the manufacturer's instructions. Genomic DNAs from gastroscopic biopsy and stool specimens of the cat were extracted and submitted to the consensus PCR to amplify Helicobacter rpoB gene. Then the DNAs from gastroscopic biopsy and stool specimens were conducted a multiplex species-specific PCR to amplify urease B gene for H. heilmannii, H. pylori and H. felis. As the results, the rapid urease test with gastroscopic biopsy was revealed positive reaction. The result of H. pylori Stool Ag assay was one red line, negative for H. pylori. The gastroscopic biopsy and stool specimen were positive reactions by the consensus PCR reaction using the RNA polymerase beta-subunit-coding gene (rpoB) to detect Helicobacter species. By multiplex species-specific PCR with gastroscopic biopsy and stool specimens, no amplification products corresponding to either H. heilmannii or H. pylori were detected, but the specimens tested were positive for H. felis. This case was confirmed as gastroenteric disease induced by H. felis infection. On our knowledge, this is a very rare report about H. felis-induced gastroenteric disease in cat and may provide a valuable data on the study of feline Helicobacter infection. PMID:27382381

  13. Integrating anthropic factors into wildcat Felis silvestris conservation in Southern Iberia landscapes

    OpenAIRE

    Ferreira, Joaquim Pedro Santos Mercês, 1969-

    2010-01-01

    Tese de doutoramento, Biologia (Biologia da Conservação), Universidade de Lisboa, Faculdade de Ciências, 2010 The European wildcat Felis silvestris is a threatened species in Europe, where suitable management of forests has been considered crucial for its conservation. However, this recommendation may not be general due to the lack of studies that test this hypothesis in the Mediterranean area, where landscapes are very different from those of centralnorth Europe. Nowadays, the...

  14. Echinococcus multilocularis detection in the intestines and feces of free-ranging domestic cats (Felis s. catus) and European wildcats (Felis s. silvestris) from northeastern France.

    Science.gov (United States)

    Umhang, Gérald; Forin-Wiart, Marie-Amélie; Hormaz, Vanessa; Caillot, Christophe; Boucher, Jean-Marc; Poulle, Marie-Lazarine; Franck, Boué

    2015-11-30

    Experimental studies have demonstrated that cats can be infected by Echinococcus multilocularis, although few data are available concerning their natural infection. This study was designed to compare experimental findings with information on the prevalence of natural E. multilocularis infections of cats in a rural high endemic area. Of 19 intestines of domestic cats (Felis s. catus) and five of European wildcats (Felis s. silvestris) analyzed by segmental sedimentation and counting technique (SSCT), infection by E. multilocularis was observed for one individual of each species, resulting in a prevalence estimated at 5%, (CI95%: 1-26) in domestic cats and at 20% (CI95%: 1-72) in wildcats. High worm burdens (680 and 7040) were noted, but comprised only immature worms. The same EmsB microsatellite profile obtained from the worms' DNA was observed in the two cats as in foxes from the same area and from other European countries. The presence of E. multilocularis DNA was diagnosed in 3.1% (10/321) of the domestic cat feces collected on the field in two villages. However, no E. multilocularis eggs were found after flotation with zinc chloride of the positive feces. The detection of DNA from E. multilocularis was thought to be due to the presence of cells from worms untied from the intestine and corresponding to prepatent infection or due to the digested metacestode. These results from E. multilocularis presence in wild and domestic cat populations agree with those previously obtained by experimental infections. These findings support that these cats play an insignificant role in E. multilocularis transmission, even in a "highly endemic" region. Nevertheless, since the presence of thick-shelled E. multilocularis eggs from cats has already been reported, the associated zoonotic risk cannot be totally ruled out, even if it is very low. PMID:26206606

  15. Echinococcus multilocularis detection in the intestines and feces of free-ranging domestic cats (Felis s. catus) and European wildcats (Felis s. silvestris) from northeastern France.

    Science.gov (United States)

    Umhang, Gérald; Forin-Wiart, Marie-Amélie; Hormaz, Vanessa; Caillot, Christophe; Boucher, Jean-Marc; Poulle, Marie-Lazarine; Franck, Boué

    2015-11-30

    Experimental studies have demonstrated that cats can be infected by Echinococcus multilocularis, although few data are available concerning their natural infection. This study was designed to compare experimental findings with information on the prevalence of natural E. multilocularis infections of cats in a rural high endemic area. Of 19 intestines of domestic cats (Felis s. catus) and five of European wildcats (Felis s. silvestris) analyzed by segmental sedimentation and counting technique (SSCT), infection by E. multilocularis was observed for one individual of each species, resulting in a prevalence estimated at 5%, (CI95%: 1-26) in domestic cats and at 20% (CI95%: 1-72) in wildcats. High worm burdens (680 and 7040) were noted, but comprised only immature worms. The same EmsB microsatellite profile obtained from the worms' DNA was observed in the two cats as in foxes from the same area and from other European countries. The presence of E. multilocularis DNA was diagnosed in 3.1% (10/321) of the domestic cat feces collected on the field in two villages. However, no E. multilocularis eggs were found after flotation with zinc chloride of the positive feces. The detection of DNA from E. multilocularis was thought to be due to the presence of cells from worms untied from the intestine and corresponding to prepatent infection or due to the digested metacestode. These results from E. multilocularis presence in wild and domestic cat populations agree with those previously obtained by experimental infections. These findings support that these cats play an insignificant role in E. multilocularis transmission, even in a "highly endemic" region. Nevertheless, since the presence of thick-shelled E. multilocularis eggs from cats has already been reported, the associated zoonotic risk cannot be totally ruled out, even if it is very low.

  16. La cryptosporidiose zoonosique humaine due à Cryptosporidium felis dans le monde

    Directory of Open Access Journals (Sweden)

    Raccurt C.P.

    2007-03-01

    Full Text Available La cryptosporidiose est l’une des principales causes de diarrhée aiguë de l’enfant dans les pays en voie de développement, et de diarrhée chronique chez les sujets immunodéprimés infectés par le virus de l’immunidéficience humaine (VIHj. L’identification de l’espèce est impossible par les méthodes conventionnelles et fait appel aux techniques de biologie moléculaire. La cryptosporidiose humaine est due le plus souvent à Cryptosporidium hominis et à Cryptosporidium parvum. Des espèces et des génotypes spécifiques d’animaux, dont Cryptosporidium felis, parasitent également l’Homme. Une revue systématique des cas humains dus à C. felis publiés dans le monde pjermet d’en décompter 58 dans toutes les régions biogéographiques, excepté l’Australie et l’Océanie. Adultes et enfants sont la cible de ce parasite. Chez ces 58 sujets parasités par C. felis, 83% sont VIH-positifs. Cette zoonose se rencontre également chez des sujets apparemment immunocompétents. Les pays en voie de développement semblent plus concernés probablement à cause d’une transmission environnementale plus présente que dans les pays développés. Cette cryptosporidiose zoonosique demande à être plus largement évaluée dans les pays tropicaux.

  17. Design of FELiChEM, the first infrared free-electron laser user facility in China

    CERN Document Server

    Li, He-Ting; Zhang, Shan-Cai; Wang, Lin; Yang, Yong-Liang

    2016-01-01

    FELiChEM is a new experimental facility under construction at University of Science and Technology of China (USTC), whose core device is two free electron laser oscillators generating middle-infrared and far-infrared laser and covering the spectral range of 2.5-200 ?m. It will be a dedicated infrared light source aiming at energy chemistry research. We present the brief design of FEL oscillators with the emphasis put on the middle-infrared oscillator. Most of the basic parameters are determined and the anticipated performance of the output radiation is given. The first light of FELiChEM is targeted for the end of 2017.

  18. Blood Meal Identification in Off-Host Cat Fleas (Ctenocephalides felis) from a Plague-Endemic Region of Uganda

    OpenAIRE

    Graham, Christine B.; Borchert, Jeff N.; Black, William C.; ATIKU, LINDA A.; Mpanga, Joseph T; Boegler, Karen A.; Moore, Sean M.; Gage, Kenneth L.; Eisen, Rebecca J.

    2013-01-01

    The cat flea, Ctenocephalides felis, is an inefficient vector of the plague bacterium (Yersinia pestis) and is the predominant off-host flea species in human habitations in the West Nile region, an established plague focus in northwest Uganda. To determine if C. felis might serve as a Y. pestis bridging vector in the West Nile region, we collected on- and off-host fleas from human habitations and used a real-time polymerase chain reaction-based assay to estimate the proportion of off-host C. ...

  19. Hallazgo de un hemopárasito eritrocítico tipo Cytauxzoon en Felis catus domesticus

    Directory of Open Access Journals (Sweden)

    Baraboglia, E. R.

    2011-07-01

    Full Text Available ResumenSe comunica el hallazgo de protozoarios eritrocíticos en Felis catusdomesticus con características morfológicas de Cytauxzoon, no pudiendoarriesgarse la especie por los detalles que exponemos, como tampoco señalar cual serían el o los vectores que actúan en nuestros casos por las particularidades que surgen de los mismos.SummaryWe show the finding of erythrocytic parasites in Felis catus domesticus similar to Cytauxzoon detected by us. It is not possible to confirm the species not either the vector.

  20. High variation in multiple paternity of domestic cats (Felis catus L.) in relation to environmental conditions.

    OpenAIRE

    Say, L.; Pontier, D; Natoli, E

    1999-01-01

    Paternity was analysed in two domestic cat (Felis catus) populations differing in habitat structure (rural versus urban) and density (234 cats km-2 versus 2,091 cats km-2). A total of 312 offspring, 76 mothers and 65 putative fathers were typed at nine microsatellite loci in the two populations. Our data showed a high rate of multiple paternity in the urban population (70-83% of litters with more than one father), whereas it was much lower in the rural population (0-22% of litters with more t...

  1. Efficacy of a new combination of fipronil and permethrin against Ctenocephalides felis flea infestation in dogs

    OpenAIRE

    Fankhauser, Becky; Dumont, Pascal; Halos, Lénaïg; Hunter, James S; Kunkle, Bruce; Everett, William R.; Chester, Theodore S; Fourie, Josephus J.; Soll, Mark D

    2015-01-01

    Background Five studies were conducted to evaluate the effect of a new combination of fipronil and permethrin on cat fleas, Ctenocephalides felis, when applied to dogs, including dogs that underwent water exposure or shampooing. Methods In each study, 16 dogs were allocated to two groups. Each dog was infested with 100 unfed adult fleas on Days −1, 7, 14, 21 and 28. Eight dogs were treated with a new topical spot-on formulation containing 6.76% w/v fipronil + 50.48% w/v permethrinon Day 0; an...

  2. Mercury in fishes of the Chassahowitzka National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From August 24 to 28, 1990, spotted seatrout (Cynoscion nebulosus), hardhead catfish (Arius felis) and gafftopsail catfish (Bagre marinus) were collected from...

  3. PCR amplification of a multi-copy mitochondrial gene (cox3) improves detection of Cytauxzoon felis infection as compared to a ribosomal gene (18S).

    Science.gov (United States)

    Schreeg, Megan E; Marr, Henry S; Griffith, Emily H; Tarigo, Jaime L; Bird, David M; Reichard, Mason V; Cohn, Leah A; Levy, Michael G; Birkenheuer, Adam J

    2016-07-30

    Cytauxzoon felis is a tick-transmitted protozoan parasite that infects felids. Clinical disease caused by acute C. felis infection rapidly progresses in domestic cats, leading to high morbidity and mortality. Accurately diagnosing cytauxzoonosis as soon as possible during acute infection would allow for earlier initiation of antiprotozoal therapy which could lead to higher survival rates. Molecular detection of parasite rRNA genes (18S) by PCR has previously been shown to be a sensitive method of diagnosing C. felis infections. Based on evidence from related apicomplexan species, we hypothesized that C. felis mitochondrial genes would exist at higher copy numbers than 18S and would be a more sensitive diagnostic target. In this study we have designed a PCR assay targeting the C. felis mitochondrial gene cytochrome c oxidase subunit III (cox3). Herein we demonstrate that (1) the cox3 PCR can detect as low as 1 copy of DNA target and can detect C. felis in samples with known mitochondrial sequence heterogeneity, (2) cox3 copy number is increased relative to 18S in blood and tissue samples from acutely infected cats, and (3) the cox3 PCR is more sensitive than 18S PCR for detection of C. felis during early infections. PMID:27369587

  4. PCR amplification of a multi-copy mitochondrial gene (cox3) improves detection of Cytauxzoon felis infection as compared to a ribosomal gene (18S).

    Science.gov (United States)

    Schreeg, Megan E; Marr, Henry S; Griffith, Emily H; Tarigo, Jaime L; Bird, David M; Reichard, Mason V; Cohn, Leah A; Levy, Michael G; Birkenheuer, Adam J

    2016-07-30

    Cytauxzoon felis is a tick-transmitted protozoan parasite that infects felids. Clinical disease caused by acute C. felis infection rapidly progresses in domestic cats, leading to high morbidity and mortality. Accurately diagnosing cytauxzoonosis as soon as possible during acute infection would allow for earlier initiation of antiprotozoal therapy which could lead to higher survival rates. Molecular detection of parasite rRNA genes (18S) by PCR has previously been shown to be a sensitive method of diagnosing C. felis infections. Based on evidence from related apicomplexan species, we hypothesized that C. felis mitochondrial genes would exist at higher copy numbers than 18S and would be a more sensitive diagnostic target. In this study we have designed a PCR assay targeting the C. felis mitochondrial gene cytochrome c oxidase subunit III (cox3). Herein we demonstrate that (1) the cox3 PCR can detect as low as 1 copy of DNA target and can detect C. felis in samples with known mitochondrial sequence heterogeneity, (2) cox3 copy number is increased relative to 18S in blood and tissue samples from acutely infected cats, and (3) the cox3 PCR is more sensitive than 18S PCR for detection of C. felis during early infections.

  5. Seroprevalence of Toxoplasma gondii and Neospora caninum in feral cats (Felis silvestris catus) in Majorca, Balearic Islands, Spain

    Science.gov (United States)

    Felids are important in the epidemiology of Toxoplasma gondii infection because they are the only hosts that can excrete environmentally-resistant oocysts. Antibodies to T. gondii and Neospora caninum were determined in serum samples from 59 feral cats (Felis silvestris catus) captured in baited tra...

  6. Implantação de colônia de Ctenocephalides felis felis (Bouché, 1835 e determinação do período de desenvolvimento dos estágios imaturos sob condições controladas / Implantation of a Ctenocephalides felis felis (Bouché, 1835 colony and determination of the development period of the immature stages under controlled conditions

    Directory of Open Access Journals (Sweden)

    Karina Carrão Castagnolli

    2008-09-01

    Full Text Available O presente estudo teve como objetivo a implantação de uma colônia de pulgas (Ctenocephalides felis felis, para determinação do período de desenvolvimento dos estágios imaturos desse inseto, quando mantido emcondições controladas. Para isto, gatos foram infestados artifi cialmente com estágios adultos de C. felis felis e mantidos em gaiolas metálicas suspensas. Diariamente, durante trinta dias, os ovos de pulgas provenientes dos gatos eram recolhidos e mantidos em estufa do tipo B.O.D., com Tode 28±1oC e umidade relativa de 75%. O tempo de eclosão larval foi em média de dois a quatro dias, as pré-pupas surgiram no período de seis a nove dias, a pupação iniciou-se com nove a onze dias e a emergência dos adultos variou de quatorze a vinte dias. Nessas condições climáticas associadas à dieta adotada, foi possível uma recuperação de adultos de aproximadamente 90%, resultados favoráveis à manutenção de umacolônia.

  7. Estudo de carcaça do bagre africano (Clarias gariepinus em diferentes categorias de peso Study of African Catfish (Clarias gariepinus carcass in different weight categories

    Directory of Open Access Journals (Sweden)

    Adriana Aparecida Pinto

    1999-11-01

    Full Text Available O objetivo deste experimento foi estudar carcaças de Clarias gariepinus, em três categorias de peso (P1=inferior a 1kg; P2=de 1 a 2kg e P3=superior a 2kg, para analisar o rendimento de carcaça com cabeça (RCC, sem cabeça (RCS, filé (RFI, músculos abdominais (RMA, partes comestíveis (RPC, tronco limpo (RTL, porcentagem de pele (PPE, cabeça (PCA, vísceras (PVI, resíduos totais (PRE e determinar a composição proximal do filé (proteína bruta, umidade, gordura e cinza. Foram utilizados 60 bagres, em delineamento inteiramente casualizado, 3 tratamentos com 20 repetições, em nível de significância de 5%. O rendimento de filé foi maior para P2 (38,61% que para P1 e P3. O rendimento das partes comestíveis e tronco limpo foram superiores para P2 (RPC=46,27% e RTL=56,67%, mas sem diferirem do P3. O rendimento de carcaça sem cabeça foi maior para P3, não diferindo de P2. O P2 foi inferior para PPE (4,64%, PCA (23,62% e PRE (53,73%, sem diferir de P3, para porcentagem de cabeça e de resíduos totais. Não houve diferença significativa para rendimentos dos músculos abdominais, carcaça com cabeça e vísceras. P2 (2,25% e 77,49% não diferiu do P1 (1,51% e 77,78% e P3 (3,03% e 76,39%, para gordura e umidade, respectivamente, enquanto, para proteína bruta, não houve diferença significativa. Conclui-se que P2 apresentou melhores rendimentos de filetagem, ao passo que a composição proximal do filé, nas três categorias de peso, foi relativamente similar, com aumento de deposição de gordura nos peixes acima de 1kg de peso vivo.The aim of this experiment was to study Clarias gariepinus carcass in three weight categories (P1=below 1kg; P2= 1 to 2kg and P3=over 2kg to analyze yield of carcass with head (YCH, without head (YCW, fillet (YF, abdominal muscles (YAM, eatable parts (YEP, dressed out (YDO and percentages of skin (PSK, head (PHE, viscera (PVI, total residues (PTR; and to determine the proximate composition of fillet (crude

  8. CHARACTERIZATION OF THE NUTRITIONAL QUALITY OF THE MEAT IN SOME SPECIES OF CATFISH: A REVIEW REVISIÓN: CARACTERIZACIÓN DE LA CALIDAD NUTRICIONAL DE LA CARNE EN ALGUNAS ESPECIES DE BAGRE

    Directory of Open Access Journals (Sweden)

    Nubia Estella Cruz Casallas

    2012-06-01

    Full Text Available Abstract. One of the most consumed fish in the world is Silurid, also called "leather fish" or catfish, whose main characteristic is the absence of intramuscular bones and scales, as well as its high productivity. In recent years, the nutritional characterization of the meat of some of these species has been carried out, finding that, although the proximal composition is within the broad ranges for fish, the fat content provides a lower proportion of polyunsaturated fatty acids (PUFA particularly as regards omega-3 (w-3, furthermore the w-6/w-3 ratio is within the proscriptions of the World Health Organization (WHO for many of these species of catfish. Likewise, the contents of eicosapentaenoic acid (EPA, docosahexaenoic acid (DHA and amino acids, minerals and vitamins reveal a high variability between individuals and species associated with the type of cultivation and dietary habits and also with the age and weight at slaughter. Furthermore quality parameters have been defined in relation to susceptibility to autolysis, oxidation and hydrolysis of fats and disturbances caused by microorganisms that cause decisive changes in the physicochemical, microbiological and sensory characteristics. This review compiles current information regarding the nutritional composition of catfish meat and the quality parameters.Resumen. Una de las carnes de pescado de mayor consumo en el mundo es la de Silúridos, también denominados peces de cuero o bagres, cuya principal característica es la ausencia de espinas intramusculares y de escamas, además de su alta productividad. En los últimos años se ha logrado realizar la caracterización nutricional de la carne de algunas de estas especies, hallándose que aunque la composición proximal se encuentra dentro de los rangos generales para peces, el contenido de grasa ofrece menor proporción de ácidos grasos poliinsaturados (AGP particularmente en lo referente a la serie omega 3 (w-3, aunque la relación w-6/w-3

  9. Heartworm (Dirofilaria immitis) disease and glomerulonephritis in a black-footed cat (Felis nigripes).

    Science.gov (United States)

    Deem, S L; Heard, D J; LaRock, R

    1998-06-01

    A 6-yr-old, 1.36-kg, intact female black-footed cat (Felis nigripes) was presented to the Veterinary Medical Teaching Hospital, University of Florida, with a history of depression, lethargy, and anorexia. Cardiac dysfunction and renal failure were diagnosed on the basis of antemortem and postmortem findings. At necropsy, heartworms (Dirofilaria immitis), glomerulonephritis, and endometritis were present. The glomerulonephritis could have been immune mediated and may have been associated with the heartworm infection or the chronic endometritis or both. Heartworm disease should be included in the list of differential diagnoses for any exotic cat housed outdoors in an endemic heartworm region that dies peracutely or has suggestive gastrointestinal or respiratory signs. Heartworm prophylaxis and annual serologic testing in exotic cats housed outdoors in heartworm endemic regions are recommended. PMID:9732037

  10. Diseases of the European wildcat (Felis silvestris Schreber, 1777) in Great Britain.

    Science.gov (United States)

    McOrist, S

    1992-12-01

    The author describes an examination conducted in collaboration with the Nature Conservancy Council of Great Britain into the status with regard to disease, conservation and genetics of the European wildcat (Felis silvestris). Feline leukaemia virus (FeLV) infection was detected by positive enzyme-linked immunosorbent assay in blood from 2 of 23 wildcats and was tested and confirmed by FeLV isolation in one of the two cats. This is the first time the virus has been clearly demonstrated in a free-living felid, other than the domestic cat. Toxoplasmosis was detected in all cats tested, but neither feline coronavirus nor feline immunodeficiency virus was detected in any sample. The genetic analysis indicated that only 8 of 42 wildcats tested were genetically distinct. These were mainly located in the western highlands of Scotland where "relict" populations may have survived. Interbreeding with domestic cats and persecution by trapping and hunting represent major threats to the survival of the European wildcat.

  11. A hybrid type undulator for far-infrared FELs at FELI

    Energy Technology Data Exchange (ETDEWEB)

    Zako, A.; Miyauchi, Y.; Koga, A. [Free Electron Laser Research Institute, Inc., Osaka (Japan)] [and others

    1995-12-31

    Two FEL facilities of the FELI are now operating in the wavelength range of 1-20 {mu}m. A 3.2-m hybrid type undulator ({lambda}{sub u}=80mm, N=40) has been designed for far-infrared FELs and will be installed in December. It can cover the wavelength of 20-60 {mu}m by changing K-value from 1 to 2.7 for a 28.0-MeV electron beam. It is composed of ferrite magnetic poles and Sm-Co permanent magnets. Commonly wound coils induce alternating magnetic field in ferrite poles. Combination of the induced field and the permanent magnet field can controls the magnetic field between the undulator gap.

  12. The parasite fauna of stray domestic cats (Felis catus) in Dubai, United Arab Emirates.

    Science.gov (United States)

    Schuster, Rolf K; Thomas, Katja; Sivakumar, Saritha; O'Donovan, Declan

    2009-07-01

    Two hundred forty feral domestic cats trapped between 2004 and 2008 in the city centre and the suburb districts of Dubai, as well as in desert biotopes, were subjected to a complete parasitological dissection. The established parasite fauna consisted of Cystoisospora felis (12.9%), Cystoisospora rivolta (9.2%), Toxoplasma/Hammondia (0.8%), Heterophyes heterophyes (2.5%), Heterophyopsis continua (0.4%), Joyeuxiella spp. (65.8%), Diplopylidium noelleri (37.1%), Hydatigera taeniaeformis (16.7%), Taenia hydatigena (0.4%), Ancylostoma ceylanicum (8.8%), Ollulanus tricuspis (0.8%), Toxocara mystax (2.9%), Toxascaris leonina (0.8%), Pterygodermatites affinis (35.0%), Centrorhynchus aluconis (4.6%), Rhipicephalus sanguineus (4.2%), Xenopsylla astia (3.8%) and Synosternus pallidus (4.2%).

  13. Perfil hematológico, bioquímico sérico e sorológico de Felis domesticus com lagochilascariose experimental Hematological, serum biochemical and serological profile of Felis domesticus with experimental lagochilascariosis

    Directory of Open Access Journals (Sweden)

    Mariana Félix de Souza Prudente

    2008-10-01

    Full Text Available No presente trabalho, avaliou-se o hemograma, diversas proteínas e enzimas séricas ou plasmáticas e a produção de anticorpos específicos em Felis domesticus, experimentalmente infectados por Lagochilascaris minor. Verificou-se nos animais infectados aumento de leucócitos totais, principalmente eosinófilos; queda do número de plaquetas; aumento de aspartato-aminotransferase e alanina-aminotransferase; e principalmente a presença de anticorpos IgG específicos para antígenos do parasita. A reação com extrato bruto de parasitas adultos mostrou-se mais específica, permitindo a discriminação de soros de animais: não infectados, com infecção por outros parasitas, e com lagochilascariose. Esta é a primeira descrição da padronização de uma reação sorológica para diagnóstico da lagochilascariose em Felis domesticus.The present study evaluated the hemogram, different proteins, plasma enzymes, serum enzymes and specific antibody production of Felis domesticus experimentally infected by Lagochilascaris minor. The infected animals were seen to present increased total leukocytes (particularly eosinophils, decreased platelet counts, increased aspartate-aminotransferase and alanine-aminotransferase and, especially, the presence of specific IgG antibodies against antigens of the parasite. The reaction with crude extract of adult parasites was shown to be more specific, thereby enabling serum discrimination between the animals: non-infected, infected with other parasites and infected with lagochilascariosis. This is the first description of the standardization of a serological reaction for diagnosing lagochilascariosis in Felis domesticus.

  14. Aspectos morfométricos do timo em gatos domésticos (Felis domesticus Morphometric aspects of the thymus in domestic cats (Felis domesticus

    Directory of Open Access Journals (Sweden)

    Camila E. Barroso

    2012-12-01

    Full Text Available O timo é um órgão linfático primário que desenvolve sua atividade em organismos jovens. Apesar de sua função ser responsável por mecanismos fundamentais na aquisição das defesas e conseqüentes respostas orgânicas, ela ainda não está totalmente esclarecida, nem tampouco as bases morfológicas que respondem por tais funções, como o processo de desenvolvimento e involução do órgão. Objetivou-se analisar e caracterizar os aspectos morfológicos do timo, tais como seu tamanho e volume, e aspectos histológicos do timo em gatos, correlacionando o sexo e o desenvolvimento etário. Doze timos provenientes de fetos de gatos domésticos (Felis domesticus sem raça definida (SRD, machos e fêmeas, separados em três grupos etários. O timo apresentou-se com uma coloração rosa-pálida e com duas porções, a torácica e a cervical, sendo que cada uma delas possuía um lobo direito e um lobo esquerdo em sua maioria. A porção torácica localizava-se em região de mediastino cranial, entre os pulmões e à base do coração. E a porção cervical estendia-se além das costelas em sentido cranial, estando localizada ventralmente à traqueia. A estrutura celular do timo demonstrou-se organizada com a presença de agregados concêntricos, os chamados corpúsculos tímicos, formados por células epiteliais, sustentada por uma cápsula de tecido conjuntivo de onde partiam septos que ao penetrar no órgão dividia-o em lóbulos. Ocorreram variações significativas quanto à lobação e as dimensões do timo entre indivíduos da mesma faixa etária, e entre sexos diferentes. Os valores relativos ao comprimento, espessura e largura, de maneira geral, apresentaram aumento, em conformidade ao desenvolvimento dos animais, mas com diferenças entre os sexos.The thymus is a primary lymphatic organ that develops its activity in young organisms. But despite its function is fundamental mechanisms responsible for the acquisition and subsequent body

  15. Differential passage rates of prey components through the gut of serval Felis serval and black-backed jackal Canis mesomelas

    Directory of Open Access Journals (Sweden)

    J.M. Bowland

    1991-09-01

    Full Text Available Feeding trials conducted on Felis serval and Canis mesomelas showed that the same prey item may appear in up to seven scats (mean=2.8, n=7. The basic assumption that prey remains of the same species in different scats represent different individuals is invalid. Therefore over-estimation of some prey species relative to others in the diet of carnivores studied can occur.

  16. Puzzling out the reproductive biology of the endangered cat’s head rockrose (Helianthemum caput-felis, Cistaceae)

    OpenAIRE

    Agulló Brotons, Jonás César; Pérez Bañón, Celeste; Crespo Villalba, Manuel Benito; Juan Gallardo, Ana Isabel

    2015-01-01

    Helianthemum caput-felis is an endangered plant species growing in fragmented habitats in the western Mediterranean basin. Reproductive traits, breeding system and pollinator assemblage were studied in its largest known (mainland) European population to improve knowledge on the reproductive biology of the species. Hand-pollination experiments were carried out to determine the breeding system. Generalized linear mixed models (GLMM) were fitted to the data to evaluate the effect of treatment on...

  17. Comparación química y funcional de tres dietas comerciales y una experimental, a través de la tasa de crecimiento de bagre de canal (Ictalurus punctatus) y su efecto en la calidad del agua

    OpenAIRE

    Sánchez Pérez, Karina Yurittsy

    2013-01-01

    Dietas de alta calidad nutricional, bajo impacto ambiental y económicamente rentables, son una necesidad apremiante para alcanzar mayor eficiencia alimenticia, tasa máxima de crecimiento de los peces y reducir los recambios en el ciclo de engorda. El objetivo del trabajo fue evaluar el crecimiento y los efectos de la proteína dietaría sobre la degradación de la calidad del agua, a través de la acumulación de nutrientes no asimilados en el agua de cultivo del bagre (Ictalurus punctatus).

  18. Feeding habits of feral cats Felis silvestris catus in the countryside of Majorca Island, Spain

    Directory of Open Access Journals (Sweden)

    J. Milan

    2010-06-01

    Full Text Available The diet of feral cats ( Felis silvestris catus in the Mediterranean island of Majorca (Spain was studied from July 2008 to June 2009 by the analysis of the scats of 75 feral cats captured in baited traps in 14 different areas. A total of 138 preys were identified in the analyzed scats. Mammals were the main group preyed on and constituted 93% both in frequency and biomass. Among them, mice were the most frequent prey consumed (55% Mus sp., 18% Apodemus sylvaticus but represented only 20% of biomass. Rat was present in 29% of scats and was the main component in terms of biomass (57%. Rabbit was found at a frequency of 6.6%, and constituted 18% of biomass. Other prey (birds, geckos and insects were found in lower frequency, and all pooled constituted only 7% of biomass. Reproductive females preyed less upon mice (20% than the other cats (77%. This may indicate that these females tended to predate upon higher preys, which may be secondary to increased energetic requirements due to pregnancy or lactation. No seasonal variations were found in any of the different parameters studied. Results indicate that rodents constitute all year round the main prey item in feral cat diet in the countryside of Majorca.

  19. Immobilizing wild mountain lions (Felis concolor) with ketamine hydrochloride and xylazine hydrochloride.

    Science.gov (United States)

    Logan, K A; Thorne, E T; Irwin, L L; Skinner, R

    1986-01-01

    A mixture of 120 mg ketamine hydrochloride (KHCL)/20 mg xylazine hydrochloride (XHCL)/ml was used to immobilize 37 wild mountain lions (Felis concolor) 46 times. Observations were recorded during 37 trials that included kittens, adult females, and adult males. Dosages were based on 11 mg KHCL and 1.8 mg XHCL/kg estimated body weight. Actual doses for 24 lions requiring a single injection for immobilization ranged from 4.7-15.8 mg KHCL/kg and 0.8-2.6 mg XHCL/kg. Induction, duration, and recovery times did not differ (P greater than 0.05) between the sex and age classes. Two kittens were overdosed with the drug combination, but the effects were not life threatening. Eleven other lions, nine of which were initially underdosed, required additional injections of the drug combination for safe handling. Immobilization was characterized initially by semi-consciousness, open eyelids, pupillary dilation, and muscle rigidity. Later, most lions appeared unconscious, muscles relaxed, and breathing slowed considerably. No convulsions or hypersalivation occurred. The KHCL/XHCL mixture given at approximately 11 mg KHCL and 1.8 mg XHCL/kg body weight proved useful for immobilizing wild mountain lions for research purposes. Suggestions for case of immobilized cats are included. PMID:3951066

  20. Domestic cats seropositive for Felis catus gammaherpesvirus 1 are often qPCR negative.

    Science.gov (United States)

    Stutzman-Rodriguez, Kathryn; Rovnak, Joel; VandeWoude, Sue; Troyer, Ryan M

    2016-11-01

    Felis catus gammaherpesvirus 1 (FcaGHV1) is a newly described virus that infects domestic cats. To identify FcaGHV1 antigens, we developed an immunofluorescent antibody assay by expressing FcaGHV1 open reading frames (ORFs) in feline cells and incubating fixed cells with sera from FcaGHV1-positive cats. Of the seven ORFs tested, ORF52 and ORF38 had the strongest, most consistent antibody responses. We used recombinant ORF52 and ORF38 proteins to develop two FcaGHV1 ELISAs. These assays were used to detect reactivity in cats previously tested by qPCR for FcaGHV1 in blood cell DNA. Results indicated 32%FcaGHV1seroprevalence, compared to 15%qPCR-evaluated prevalence (n=133);all but one qPCR positive animal was seropositive. ELISA results confirmed infection risk factors previously identified by qPCR: geographic location, male sex, and adult age. These data suggest that FcaGHV1is a common infection of domestic cats that has a seropositive but often qPCR negative state characteristic of herpesviral latency. PMID:27540873

  1. Performance of an undulator for visible and UV FELs at FELI

    Energy Technology Data Exchange (ETDEWEB)

    Miyauchi, Y.; Zako, A.; Koga, A. [Free Electron Laser Research Institute, Inc., Osaka (Japan)] [and others

    1995-12-31

    Two infrared free electron lasers (FELs) of the FELI project are now operating in the wavelength range of 1-20{mu}m. A 2.68-m undulator has been constructed for visible and UV FELs covering the wavelength of 1-0.2{mu}m for 100-165 MeV electron beams. It generates alternating, horizontal magnetic field, and wiggles electron beam on a vertical plane. The undulator length and period are 2.68m and 40mm, respectively. The gap of undulator magnets can be changed remotely by using servomotors with an accuracy of 1 {mu}m from the control room. The maximum K-value and related magnetic field strength are 1.9 and 0.5T, respectively, when its gap is set to the minimum value of 16mm. In order to minimize magnetic field reduction due to radiation damage, Sm-Co permanent magnet was adopted. Its structure and the results of magnetic field measurement will be reported.

  2. Mesothelioma in Two Nondomestic Felids: North American Cougar (Felis concolor and Cheetah (Acinonyx jubatus

    Directory of Open Access Journals (Sweden)

    Amanda Whiton

    2013-01-01

    Full Text Available A 15-year-old male North American cougar (Felis concolor presented with a 2-day history of anorexia, restlessness, and dyspnea. White blood cell count ( cells/μL and absolute segmented neutrophil count ( cells/μL were increased, and BUN (143 mg/dL, creatinine (6.3 mg/dL, and phosphorus (8.5 mg/dL concentrations indicated chronic renal disease. Thoracic radiographs showed severe pleural and pericardial effusion. During attempts to remove the fluid, cardiac tamponade developed and the cat died. At necropsy, nodular masses decorated the pericardium at the level of the base of the heart. The final microscopic diagnosis was mesothelioma of the pericardium, tunica adventitia of the main pulmonary artery, left auricle epicardium, and left ventricular epicardium. A 15-year-old female cheetah (Acinonyx jubatus was evaluated for acute respiratory distress. The white blood cell count ( cells/μL and absolute segmented neutrophil count ( cells/μL were increased. Radiographically pleural effusion and a cranial thoracic mass were seen. The cheetah was euthanized, and a gross diagnosis of disseminated pleural mesothelioma with thoracic effusion was made. Histologically, pleural mesothelioma was confirmed with local invasion of the lung and pulmonary arterial emboli and infarction. In both cases, a diagnosis of mesothelioma was made based on cellular morphology, microscopic architecture, and neoplastic cell coexpression of cytokeratin and vimentin.

  3. Gastrointestinal Helminthic Parasites in Stray Cats (Felis catus from North of Iran

    Directory of Open Access Journals (Sweden)

    A Rezaei-Doust

    2007-08-01

    Full Text Available Background: Cats play a crucial role in the epidemiology of gastrointestinal helminthic parasites and also play a major role in transmitting of these parasites through faecal contamination of soil, food or water. The aim of this study was to determine the species of gastrointestinal helminthes parasites in stray cats from a rural area of Bandar-e-Anzali, Iran.Method: Gastrointestinal helminthes were collected from 50 necropsied stray cats (Felis catus after capturing them by trapping from different regions of the city and humanely euthanatized in Bandar-e-Anzali, a port in the Caspian Sea in northern Iran, from March to November 2003. Results: The prevalence of infection was 90%, with those of individual parasites being Diplopylidium nolleri 54%, Phy­saloptera praeputialis 32%, Ancylostoma tubaeforme 20%, Joyeuxiella pasqualei 10%, Toxocara cati 8%, Pterygoderma­tites affinis 6%, Ancylostoma caninum 4%, and Taenia taeniaeformis 2%. Concurrent infections with two or more parasites were recorded in 34% of the individuals. In relation to the sex, the differences were not significant. Conclusion: P. praeputialis, T. cati, D. nolleri and sometime J. pasqualei are the commonest Helminthes in cats. This is the first reported isolation of P. affinis and A. caninum infections from cats in Iran.

  4. Efficacy of indoxacarb applied to cats against the adult cat flea, Ctenocephalides felis, flea eggs and adult flea emergence

    OpenAIRE

    Dryden, Michael W; Payne, Patricia A; Smith, Vicki; Heaney, Kathleen; Sun, Fangshi

    2013-01-01

    Background A study was conducted to evaluate the effect of indoxacarb applied to cats on adult cat fleas, Ctenocephalides felis, flea egg production and adult flea emergence. Methods Sixteen cats were selected for the study and allocated to two treatment groups. Eight cats were treated with a 19.5% w/v topical spot-on solution of indoxacarb on day 0 and eight cats served as untreated controls. Each cat was infested with 50 fleas on Days -2, 7, 14, 21, 28, 35 and 42. On Days 1, 2, and 3, and a...

  5. Detection of Rickettsia in Rhipicephalus sanguineus ticks and Ctenocephalides felis fleas from southeastern Tunisia by reverse line blot assay.

    Science.gov (United States)

    Khrouf, Fatma; M'Ghirbi, Youmna; Znazen, Abir; Ben Jemaa, Mounir; Hammami, Adnene; Bouattour, Ali

    2014-01-01

    Ticks (n = 663) and fleas (n = 470) collected from domestic animals from southeastern Tunisia were screened for Rickettsia infection using reverse line blot assay. Evidence of spotted fever group Rickettsia was obtained. We detected Rickettsia felis in fleas, Rickettsia massiliae Bar 29 and the Rickettsia conorii Israeli spotted fever strain in ticks, and Rickettsia conorii subsp. conorii and Rickettsia spp. in both arthropods. The sensitivity of the adopted technique allowed the identification of a new association between fleas and R. conorii subsp. conorii species. The presence of these vector-borne Rickettsia infections should be considered when diagnosing this disease in humans in Tunisia.

  6. Functional Analyses of Bitter Taste Receptors in Domestic Cats (Felis catus.

    Directory of Open Access Journals (Sweden)

    Weiwei Lei

    Full Text Available Cats are obligate carnivores and under most circumstances eat only animal products. Owing to the pseudogenization of one of two subunits of the sweet receptor gene, they are indifferent to sweeteners, presumably having no need to detect plant-based sugars in their diet. Following this reasoning and a recent report of a positive correlation between the proportion of dietary plants and the number of Tas2r (bitter receptor genes in vertebrate species, we tested the hypothesis that if bitter perception exists primarily to protect animals from poisonous plant compounds, the genome of the domestic cat (Felis catus should have lost functional bitter receptors and they should also have reduced bitter receptor function. To test functionality of cat bitter receptors, we expressed cat Tas2R receptors in cell-based assays. We found that they have at least 7 functional receptors with distinct receptive ranges, showing many similarities, along with some differences, with human bitter receptors. To provide a comparative perspective, we compared the cat repertoire of intact receptors with those of a restricted number of members of the order Carnivora, with a range of dietary habits as reported in the literature. The numbers of functional bitter receptors in the terrestrial Carnivora we examined, including omnivorous and herbivorous species, were roughly comparable to that of cats thereby providing no strong support for the hypothesis that a strict meat diet influences bitter receptor number or function. Maintenance of bitter receptor function in terrestrial obligate carnivores may be due to the presence of bitter compounds in vertebrate and invertebrate prey, to the necessary role these receptors play in non-oral perception, or to other unknown factors. We also found that the two aquatic Carnivora species examined had fewer intact bitter receptors. Further comparative studies of factors driving numbers and functions of bitter taste receptors will aid in

  7. Recent status of five FEL facilities and application research at FELI

    International Nuclear Information System (INIS)

    Four FEL facilities FEL-1, FEL-2, FEL-3 and FEL-4 achieved lasing at FELI in Oct. 1994, in Feb. 1995, in Dec. 1995 and in Oct. 1996, respectively. Two IR facilities FEL-1 and FEL-2 are open for users to supply several MW-level FELs covering the wavelength range of 1-20μm every other week. Total operation time in 1996 was about 2400 hours. The visible- and UV-facility FEL-3 has broken the world record for the shortest wavelength oscillation of linac-based FELs with a thermionic gun up to 0.278μm. An FIR facility FEL-4 (20-80μm) was installed at the 33-MeV beam line, downstream of FEL-1. The electron beam once lased at FEL-1 is used for lasing at FEL-4. The electron beam of the 165-MeV linac consists of a train of several picosecond micropulse repeating at 22.3125MHz or at 89.25MHz. The train of the micropulse continues for 24μs (macropulse) and the repetition rate of the macropulse is 10Hz or 20Hz. The number of the micropulse in a macropulse is 2140 with a micropulse separation of 11.2ns. The maximum average power of the IR-FEL will be a few W. The FEL beams are delivered from the optical cavities to the diagnostics room and four user's rooms (eighteen stations) through the pipe lines. The beam can be shared by using fan-shaped Au-coated mirrors to several stations including two manipulators simultaneously for bio-medical and semiconductor applications. An FIR-FEL facility FEL-5 (50-100μm) was installed at the beam line of the 20-MeV linac with an RF gun. FEL-5 is in the commissioning stage. (author)

  8. Phenotypic and Molecular Characterization of Domestic Cat (Felis catus) Spermatogonial Stem Cells.

    Science.gov (United States)

    Powell, Robin H; Galiguis, Jason; Biancardi, Monica N; Pope, C Earle; Leibo, Stanley P; Wang, Guoshun; Gómez, Martha C

    2016-07-01

    In many mammalian species, surface markers have been used to obtain enriched populations of spermatogonial stem cells (SSCs) for assisted reproduction and other applications; however, little is known about the expression patterns of feline SSCs. In this study, we assessed expression of the SSC surface markers commonly used in other species, KIT, ITGA6, CD9, GFRalpha1, ADGRA3, and THY1, in addition to the less frequently used pluripotent markers TRA-1-60, TRA-1-81, SSEA-1, and SSEA-4 in SSCs of both prepubertal and adult domestic cats (Felis catus). To further characterize cat SSCs, we sorted cells using SSC-specific markers and evaluated the expression of the pluripotent transcription factors NANOG, POU5F1, and SOX2 and the proto-oncogene MYC within these populations. We concluded that SSC surface markers used in other mammalian species were not specific for identifying cat SSCs. However, the pluripotent markers we evaluated were more specific to cat spermatogonia, and the presence of SSEA-1 and SSEA-4 in fewer and primarily individual cells suggests that these two markers may be used for enrichment of cat SSCs. The expression of pluripotent transcription factors at mRNA level by single-stained cells positive for SSEA-4 and by dual-stained cells positive for both GFRalpha1 and SSEA-4 reflects the undifferentiated stage of cat SSCs. The absence of transcription factors in double-stained cells positive for only one marker implies the loss of the stem cell-like identity with the loss of either GFRalpha1 or SSEA-4. Further investigation is warranted to elucidate the biological characteristics of these spermatogonial subpopulations. PMID:27281702

  9. Survey on parasitic infections in wildcat (Felis silvestris silvestris Schreber, 1777) by scat collection.

    Science.gov (United States)

    Napoli, Ettore; Anile, Stefano; Arrabito, Carmelo; Scornavacca, Davide; Mazzamuto, Maria Vittoria; Gaglio, Gabriella; Otranto, Domenico; Giannetto, Salvatore; Brianti, Emanuele

    2016-01-01

    Wildcats are endangered felid species living in Europe, Asia, and Africa. Regrettably, scientific information on parasites of wildcats is particularly meager and they often rely on data gained by necropsies of a small number of animals. In the present study, scat collection was used to assess the parasite spectrum of European wildcats living in the Etna Park (Sicily, Italy). Scat collection was performed from May to September 2010 by weekly walking four transects for a total of 391 km. Samples were then analyzed by flotation and sedimentation techniques to investigate wildcat parasitic fauna. A total of 121 scats of wildcats were collected, and parasitic forms (i.e., oocysts, eggs, and larvae) were retrieved in 110 (90.9 %) of the samples. Parasites found were Physaloptera sp. (52.1 %), tapeworms (45.5 %), Toxocara cati (43.8 %), Eucoleus aerophilus (27.3 %), Ancylostoma sp. (22.3 %), Troglostrongylus brevior (15.7 %), trematodes (9.9 %), Isospora felis (4.1 %), Cylicospirura sp. (1.7 %), and Acanthocephala (0.8 %). The prevalence of endoparasitic infections herein recorded is similar to that described in other studies conducted using necropsy technique. The species richness of parasites found in the present survey, with a total of nine helminths and one protozoon, is the highest ever reported for wildcat in Europe. Scat collection and examination are reliable and rapid non-invasive tools which can be used in a systematic survey design to study the parasite spectrum of wildcat as well as that of other endangered wild species.

  10. Molecular characterisation of Cryptosporidium and Giardia in cats (Felis catus) in Western Australia.

    Science.gov (United States)

    Yang, Rongchang; Ying, Joyce Lau Jie; Monis, Paul; Ryan, Una

    2015-08-01

    Little is known of the prevalence of Cryptosporidium and Giardia in domestic cats in Western Australia and their potential role as zoonotic reservoirs for human infection. In the present study, a total of 345 faecal samples from four different sources were screened for the presence of Cryptosporidium and Giardia by PCR and genotyped by sequence analysis. Oocyst numbers and cyst numbers for Cryptosporidium and Giardia respectively were also determined using quantitative PCR assays. Cryptosporidium and Giardia were detected in 9.9% (95% CI 6.7-13.0) and 10.1% (95% CI 7.0-13.3) of cats in Western Australia respectively. Sequence analysis at the 18S rRNA locus identified five Cryptosporidium species/genotypes; C. felis (n = 8), C. muris (n = 1), C. ryanae (n = 1), Cryptosporidium rat genotype III (n = 5) and a novel genotype most closely related to Cryptosporidium rat genotype III in one isolate. This is the first report of C. ryanae and Cryptosporidium rat genotype III in cats. For Giardia, assemblage F the most commonly identified species, while only 1 assemblage sequence was detected. Since most human cases of cryptosporidiosis are caused by C. parvum and C. hominis and human cases of giardiasis are caused by G. duodenalis assemblage A and B, the domestic cats in the present study are likely to be of low zoonotic risk to pet owners in Perth. Risk analyses identified that elderly cats (more than 6 years) were more prone to Cryptosporidium and Giardia infections than kittens (less than 6 months) (P = 0.009). Clinical symptoms were not associated with the prevalence of Cryptosporidium and Giardia infections in cats. PMID:25959691

  11. Extent of linkage disequilibrium in the domestic cat, Felis silvestris catus, and its breeds.

    Directory of Open Access Journals (Sweden)

    Hasan Alhaddad

    Full Text Available Domestic cats have a unique breeding history and can be used as models for human hereditary and infectious diseases. In the current era of genome-wide association studies, insights regarding linkage disequilibrium (LD are essential for efficient association studies. The objective of this study is to investigate the extent of LD in the domestic cat, Felis silvestris catus, particularly within its breeds. A custom illumina GoldenGate Assay consisting of 1536 single nucleotide polymorphisms (SNPs equally divided over ten 1 Mb chromosomal regions was developed, and genotyped across 18 globally recognized cat breeds and two distinct random bred populations. The pair-wise LD descriptive measure (r(2 was calculated between the SNPs in each region and within each population independently. LD decay was estimated by determining the non-linear least-squares of all pair-wise estimates as a function of distance using established models. The point of 50% decay of r(2 was used to compare the extent of LD between breeds. The longest extent of LD was observed in the Burmese breed, where the distance at which r(2 ≈ 0.25 was ∼380 kb, comparable to several horse and dog breeds. The shortest extent of LD was found in the Siberian breed, with an r(2 ≈ 0.25 at approximately 17 kb, comparable to random bred cats and human populations. A comprehensive haplotype analysis was also conducted. The haplotype structure of each region within each breed mirrored the LD estimates. The LD of cat breeds largely reflects the breeds' population history and breeding strategies. Understanding LD in diverse populations will contribute to an efficient use of the newly developed SNP array for the cat in the design of genome-wide association studies, as well as to the interpretation of results for the fine mapping of disease and phenotypic traits.

  12. Activity of a deltamethrin shampoo against Ctenocephalides felis and Rhipicephalus sanguineus in dogs.

    Science.gov (United States)

    Franc, M; Cadiergues, M C

    1999-03-15

    A controlled clinical trial was undertaken to assess the efficacy of an application of a 0.07% deltamethrin shampoo against fleas and ticks in dogs. Twenty beagles, housed in separate cages, were randomly allocated into two groups of 10. The dogs were infested with 50 Ctenocephalides felis and 50 Rhipicephalus sanguineus each, and parasites were counted 24 h (fleas) and 72 h (ticks) later (Day 0). Dogs, when state, were then treated with 25 ml of a shampoo containing 0.07% deltamethrin (Group 1). Dogs in Group 2 were the controls. Fleas and ticks were counted 24 h (Day 1) and 48 h (Day 2) after the shampoo was applied, and the parasites were then removed. All the dogs were reinfested with fleas and ticks on Days 2, 7, 9, 14, 16 and 20. Parasites were counted 24 and 48 h after each reinfestation. Effectiveness against fleas was calculated 24 h after infestation, and against ticks 48 h after infestation. This study showed that the application of 25 ml of shampoo containing 0.07% deltamethrin to beagle dogs weighing between 10.2 and 12 kg was very well tolerated. It controlled the parasites present on the animals at the time of application: with an efficacy of 100% against fleas 24 h after treatment and an efficacy of 95% against ticks 48 h after treatment. The treatment protected against flea reinfestations with an efficacy of 100% during the first week, >98% in the second week and >95% in the third week. It also gave >99% protection against tick reinfestations in the first week and >96% in the second week.

  13. Survey on parasitic infections in wildcat (Felis silvestris silvestris Schreber, 1777) by scat collection.

    Science.gov (United States)

    Napoli, Ettore; Anile, Stefano; Arrabito, Carmelo; Scornavacca, Davide; Mazzamuto, Maria Vittoria; Gaglio, Gabriella; Otranto, Domenico; Giannetto, Salvatore; Brianti, Emanuele

    2016-01-01

    Wildcats are endangered felid species living in Europe, Asia, and Africa. Regrettably, scientific information on parasites of wildcats is particularly meager and they often rely on data gained by necropsies of a small number of animals. In the present study, scat collection was used to assess the parasite spectrum of European wildcats living in the Etna Park (Sicily, Italy). Scat collection was performed from May to September 2010 by weekly walking four transects for a total of 391 km. Samples were then analyzed by flotation and sedimentation techniques to investigate wildcat parasitic fauna. A total of 121 scats of wildcats were collected, and parasitic forms (i.e., oocysts, eggs, and larvae) were retrieved in 110 (90.9 %) of the samples. Parasites found were Physaloptera sp. (52.1 %), tapeworms (45.5 %), Toxocara cati (43.8 %), Eucoleus aerophilus (27.3 %), Ancylostoma sp. (22.3 %), Troglostrongylus brevior (15.7 %), trematodes (9.9 %), Isospora felis (4.1 %), Cylicospirura sp. (1.7 %), and Acanthocephala (0.8 %). The prevalence of endoparasitic infections herein recorded is similar to that described in other studies conducted using necropsy technique. The species richness of parasites found in the present survey, with a total of nine helminths and one protozoon, is the highest ever reported for wildcat in Europe. Scat collection and examination are reliable and rapid non-invasive tools which can be used in a systematic survey design to study the parasite spectrum of wildcat as well as that of other endangered wild species. PMID:26377843

  14. Activity of a deltamethrin shampoo against Ctenocephalides felis and Rhipicephalus sanguineus in dogs.

    Science.gov (United States)

    Franc, M; Cadiergues, M C

    1999-03-15

    A controlled clinical trial was undertaken to assess the efficacy of an application of a 0.07% deltamethrin shampoo against fleas and ticks in dogs. Twenty beagles, housed in separate cages, were randomly allocated into two groups of 10. The dogs were infested with 50 Ctenocephalides felis and 50 Rhipicephalus sanguineus each, and parasites were counted 24 h (fleas) and 72 h (ticks) later (Day 0). Dogs, when state, were then treated with 25 ml of a shampoo containing 0.07% deltamethrin (Group 1). Dogs in Group 2 were the controls. Fleas and ticks were counted 24 h (Day 1) and 48 h (Day 2) after the shampoo was applied, and the parasites were then removed. All the dogs were reinfested with fleas and ticks on Days 2, 7, 9, 14, 16 and 20. Parasites were counted 24 and 48 h after each reinfestation. Effectiveness against fleas was calculated 24 h after infestation, and against ticks 48 h after infestation. This study showed that the application of 25 ml of shampoo containing 0.07% deltamethrin to beagle dogs weighing between 10.2 and 12 kg was very well tolerated. It controlled the parasites present on the animals at the time of application: with an efficacy of 100% against fleas 24 h after treatment and an efficacy of 95% against ticks 48 h after treatment. The treatment protected against flea reinfestations with an efficacy of 100% during the first week, >98% in the second week and >95% in the third week. It also gave >99% protection against tick reinfestations in the first week and >96% in the second week. PMID:10206106

  15. Biología reproductiva del bagre cominate Occidentarius platypogon (Pisces: Ariidae en el sureste del golfo de California Reproductive biology of the cominate sea catfish Occidentarius platypogon (Pisces: Ariidae from the southeastern Gulf of California

    Directory of Open Access Journals (Sweden)

    Felipe Amezcua

    2012-07-01

    Full Text Available En el sureste del golfo de California, el bagre cominate presenta gran abundancia y es explotado comercialmente. Sin embargo, estudios sobre su biología son escasos y no existen normas que regulen su explotación en México. En este trabajo se describe la biología reproductiva de Occidentarius platypogon. Se capturaron 480 organismos con redes agalleras entre octubre 2008 y diciembre 2009. El estado de madurez gonadal se determinó macroscópicamente. Esta especie tiene una época de desove de mayo a agosto con un desarrollo sincrónico en dos lotes. La fecundidad media fue de 37 ovocitos maduros. La baja fecundidad se debe a que esta especie presenta cuidado parental con incubación oral por parte de los machos, lo cual reduce su mortalidad natural en las primeras etapas de desarrollo. La talla de primera madurez fue de 37,6 cm y la longitud estimada de primera captura de la pesquería artesanal con redes de enmalle fue de 23,4 cm, lo que indica la captura de organismos que aún no alcanzan su talla reproductiva. La mayoría de los organismos capturados fueron machos incubando, por lo tanto, podría ser alta la mortalidad por pesca de juveniles.The cominate sea catfish from the southeastern Gulf of California is an important species that is commercially exploited. However, studies on biology of this species are scarce; therefore, harvest regulations do not exist for this species in Mexico. The aim of this work was to describe reproductive biology of Occidentarius platypogon. Gillnet samples were carried out from October 2008 to December 2009. In total, 480 fish were dissected; gender sex and gonad maturity stage were determined macroscopically. Results indicated that the spawning season runs from May to August and generally with synchronic gonad development. Fecundity of the chihuil sea catfish was 37, which is low because this species shows parental care via oral incubation of fry, reducing mortality. Estimated length at first maturity is 37

  16. ISOLATION AND GENETIC CHARACTERIZATION OF TOXOPLASMA GONDII FROM RACCOONS (PROCYON LOTOR), CATS (FELIS DOMESTICUS), STRIPED SKUNK (MEPHITIS MEPHITIS), BLACK BEAR (URSUS AMERICANUS), AND COUGAR (PUMA CONCOLOR) FROM CANADA

    Science.gov (United States)

    Viable Toxoplasma gondii was isolated by bioassay in mice from tissues of 2 feral cats ( Felis domesticus), 2 raccoons (Procyon lotor), a skunk (Mephitis mephitis) trapped in remote locations in Manitoba, Canada, and a black bear (Ursus americanus ) from Kuujjuaq, northern Quebec, Canada. Geno...

  17. A new atypical genotype mouse virulent strain of Toxoplasma gondii isolated from the heart of a wild caught puma (Felis concolor) from Durango, Mexico

    Science.gov (United States)

    Nothing is known of the genetic diversity of Toxoplasma gondii circulating in wildlife in Mexico. In the present study, a mouse virulent T. gondii strain was isolated from the heart of a wild puma (Felis concolor). The puma was found roaming in outskirt of Durango City, Mexico and tranquailized for ...

  18. Toxoplasmosis in sand cats (Felis margarita) and other animals in the Breeding Centre for Endangered Arabian Wildlife in the United Arab Emirates

    Science.gov (United States)

    The Sand cat (Felis margarita) is a small-sized felid occurring in the United Arab Emirates (UAE). The Sand cat captive breeding program at the Breeding Centre for Endangered Arabian Wildlife (BCEAW), Sharjah, UAE, has experienced high newborn mortality rates, and congenital toxoplasmosis was recent...

  19. Aspectos morfométricos do timo em gatos domésticos (Felis domesticus

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    Camila E. Barroso

    2012-12-01

    Full Text Available O timo é um órgão linfático primário que desenvolve sua atividade em organismos jovens. Apesar de sua função ser responsável por mecanismos fundamentais na aquisição das defesas e conseqüentes respostas orgânicas, ela ainda não está totalmente esclarecida, nem tampouco as bases morfológicas que respondem por tais funções, como o processo de desenvolvimento e involução do órgão. Objetivou-se analisar e caracterizar os aspectos morfológicos do timo, tais como seu tamanho e volume, e aspectos histológicos do timo em gatos, correlacionando o sexo e o desenvolvimento etário. Doze timos provenientes de fetos de gatos domésticos (Felis domesticus sem raça definida (SRD, machos e fêmeas, separados em três grupos etários. O timo apresentou-se com uma coloração rosa-pálida e com duas porções, a torácica e a cervical, sendo que cada uma delas possuía um lobo direito e um lobo esquerdo em sua maioria. A porção torácica localizava-se em região de mediastino cranial, entre os pulmões e à base do coração. E a porção cervical estendia-se além das costelas em sentido cranial, estando localizada ventralmente à traqueia. A estrutura celular do timo demonstrou-se organizada com a presença de agregados concêntricos, os chamados corpúsculos tímicos, formados por células epiteliais, sustentada por uma cápsula de tecido conjuntivo de onde partiam septos que ao penetrar no órgão dividia-o em lóbulos. Ocorreram variações significativas quanto à lobação e as dimensões do timo entre indivíduos da mesma faixa etária, e entre sexos diferentes. Os valores relativos ao comprimento, espessura e largura, de maneira geral, apresentaram aumento, em conformidade ao desenvolvimento dos animais, mas com diferenças entre os sexos.

  20. Estudo morfométrico de rins em felinos domésticos (Felis catus

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    Rafael G. Agopian

    2016-04-01

    Full Text Available Resumo A saúde renal tem ao longo dos últimos anos chamado atenção dos médicos veterinários, pois o comprometimento deste órgão na insuficiência renal crônica se apresenta como a maior causa de morbidade e mortalidade em felinos. O presente estudo propõe a caracterização biométrica dos rins de gatos, Felis catus, sob os aspectos macroscópicos (comprimento, altura, largura, peso e volume, mesoscópico (altura do córtex e da medula, e a sua inter-relação e microscópica (volume glomerular, a fim de se estabelecer possíveis diferenças decorrentes de idade, sexo e simetria bilateral. Foram utilizados, rins de 30 animais da espécie Felis catus (gato deméstico, sendo 15 machos e 15 fêmeas com idade variando entre 3 meses a 15 anos, divididos em três grupos: grupo 1 (3-9 meses, grupo 2 (3-5 anos e grupo 3 (acima de 10 anos. Os rins foram fotografados, pesados, mensurados e processados para histologia. O volume glomerular foi obtido através de estereologia. Os dados macroscópicos permitiram observar uma imparcialidade morfométrica e morfológica quando comparados os lados, independente do grupo e do sexo, sobre o comprimento, altura, largura, peso e volume dos rins. Para o sexo foi detectado diferença significante entre machos e fêmeas independente da faixa etária para comprimento, altura, peso e volume, com exceção da largura. Considerando a idade houve diferença significativa para todos os parâmetros: comprimento, altura, largura, peso e volume. Nas mensurações de córtex, medula e sua inter-relação, quando considerada a simetria bilateral, não foi detectada diferença significante entre rim direito e esquerdo. Para o sexo, não foi detectada diferença entre machos e fêmeas na mesoscopia no córtex, na medula e na inter-relação córtex/medula. Quanto à idade, a mensuração da altura do córtex e a inter-relação córtex/medula apresentou diferença significante, porém essa diferença não foi estabelecida

  1. Survival of feral cats, Felis catus (Carnivora: Felidae), on Mauna Kea, Hawai'i, based on tooth cementum lines

    Science.gov (United States)

    Danner, Raymond M.; Farmer, Chris; Hess, Steven C.; Stephens, Robert M.; Banko, Paul C.

    2010-01-01

    Feral cats (Felis catus) have spread throughout anthropogenic and insular environments of the world. They now threaten many species of native wildlife with chronic depredation. Knowledge of feral cat population dynamics is necessary to understand their ecological effects and to develop effective control strategies. However, there are few studies worldwide regarding annual or lifetime survival rates in remote systems, and none on Pacific islands. We constructed the age distribution and estimated survival of feral cats in a remote area of Hawai'i Island using cementum lines present in lower canine teeth. Our data suggest annual cementum line formation. A log-linear model estimated annual survival ≥ 1 yr of age to be 0.647. Relatively high survival coupled with high reproductive output allows individual cats to affect native wildlife for many years and cat populations to rebound quickly after control efforts.

  2. Differential passage rates of prey components through the gut of serval Felis serval and black-backed jackal Canis mesomelas

    OpenAIRE

    J.M. Bowland; A.E. Rowland

    1991-01-01

    Feeding trials conducted on Felis serval and Canis mesomelas showed that the same prey item may appear in up to seven scats (mean=2.8, n=7). The basic assumption that prey remains of the same species in different scats represent different individuals is invalid. Therefore over-estimation of some prey species relative to others in the diet of carnivores studied can occur.

  3. Multidimensional effects of biologically synthesized silver nanoparticles in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma A549 cells

    OpenAIRE

    Gurunathan, Sangiliyandi; Jeong, Jae-Kyo; Han, Jae Woong; Zhang, Xi-Feng; Park, Jung Hyun; Kim, Jin-Hoi

    2015-01-01

    Silver nanoparticles (AgNPs) are prominent group of nanomaterials and are recognized for their diverse applications in various health sectors. This study aimed to synthesize the AgNPs using the leaf extract of Artemisia princeps as a bio-reductant. Furthermore, we evaluated the multidimensional effect of the biologically synthesized AgNPs in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma (A549) cells. UV-visible (UV–vis) spectroscopy confirmed the synthesis ...

  4. Rickettsia typhi IN RODENTS AND R. felis IN FLEAS IN YUCATÁN AS A POSSIBLE CAUSAL AGENT OF UNDEFINED FEBRILE CASES

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    Gaspar PENICHE-LARA

    2015-04-01

    Full Text Available Rickettsia typhi is the causal agent of murine typhus; a worldwide zoonotic and vector-borne infectious disease, commonly associated with the presence of domestic and wild rodents. Human cases of murine typhus in the state of Yucatán are frequent. However, there is no evidence of the presence of Rickettsia typhi in mammals or vectors in Yucatán. The presence of Rickettsia in rodents and their ectoparasites was evaluated in a small municipality of Yucatán using the conventional polymerase chain reaction technique and sequencing. The study only identified the presence of Rickettsia typhi in blood samples obtained from Rattus rattus and it reported, for the first time, the presence of R. felis in the flea Polygenis odiosus collected from Ototylomys phyllotis rodent. Additionally, Rickettsia felis was detected in the ectoparasite Ctenocephalides felis fleas parasitizing the wild rodent Peromyscus yucatanicus. This study’s results contributed to a better knowledge of Rickettsia epidemiology in Yucatán.

  5. Rickettsia typhi IN RODENTS AND R. felis IN FLEAS IN YUCATÁN AS A POSSIBLE CAUSAL AGENT OF UNDEFINED FEBRILE CASES

    Science.gov (United States)

    PENICHE-LARA, Gaspar; DZUL-ROSADO, Karla; PÉREZ-OSORIO, Carlos; ZAVALA-CASTRO, Jorge

    2015-01-01

    Rickettsia typhi is the causal agent of murine typhus; a worldwide zoonotic and vector-borne infectious disease, commonly associated with the presence of domestic and wild rodents. Human cases of murine typhus in the state of Yucatán are frequent. However, there is no evidence of the presence of Rickettsia typhi in mammals or vectors in Yucatán. The presence of Rickettsia in rodents and their ectoparasites was evaluated in a small municipality of Yucatán using the conventional polymerase chain reaction technique and sequencing. The study only identified the presence of Rickettsia typhi in blood samples obtained from Rattus rattus and it reported, for the first time, the presence of R. felis in the flea Polygenis odiosus collected from Ototylomys phyllotis rodent. Additionally, Rickettsia felis was detected in the ectoparasite Ctenocephalides felis fleas parasitizing the wild rodent Peromyscus yucatanicus. This study’s results contributed to a better knowledge of Rickettsia epidemiology in Yucatán. PMID:25923891

  6. Antifungal activity of extracts from Atacama Desert fungi against Paracoccidioides brasiliensis and identification of Aspergillus felis as a promising source of natural bioactive compounds.

    Science.gov (United States)

    Mendes, Graziele; Gonçalves, Vívian N; Souza-Fagundes, Elaine M; Kohlhoff, Markus; Rosa, Carlos A; Zani, Carlos L; Cota, Betania B; Rosa, Luiz H; Johann, Susana

    2016-03-01

    Fungi of the genus Paracoccidioides are responsible for paracoccidioidomycosis. The occurrence of drug toxicity and relapse in this disease justify the development of new antifungal agents. Compounds extracted from fungal extract have showing antifungal activity. Extracts of 78 fungi isolated from rocks of the Atacama Desert were tested in a microdilution assay against Paracoccidioides brasiliensis Pb18. Approximately 18% (5) of the extracts showed minimum inhibitory concentration (MIC) values ≤ 125.0 µg/mL. Among these, extract from the fungus UFMGCB 8030 demonstrated the best results, with an MIC of 15.6 µg/mL. This isolate was identified as Aspergillus felis (by macro and micromorphologies, and internal transcribed spacer, β-tubulin, and ribosomal polymerase II gene analyses) and was grown in five different culture media and extracted with various solvents to optimise its antifungal activity. Potato dextrose agar culture and dichloromethane extraction resulted in an MIC of 1.9 µg/mL against P. brasiliensis and did not show cytotoxicity at the concentrations tested in normal mammalian cell (Vero). This extract was subjected to bioassay-guided fractionation using analytical C18RP-high-performance liquid chromatography (HPLC) and an antifungal assay using P. brasiliensis. Analysis of the active fractions by HPLC-high resolution mass spectrometry allowed us to identify the antifungal agents present in the A. felis extracts cytochalasins. These results reveal the potential of A. felis as a producer of bioactive compounds with antifungal activity. PMID:27008375

  7. Influence de la température sur le développement de la puce africaine du chat Ctenocephalides felis strongylus (Jordan, 1925 (Siphonaptera : Pulicidae

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    Yao K.P.

    2010-06-01

    Full Text Available Ctenocephalides felis (Bouché, 1835 communément appelée “puce du chat” présente deux sous-espèces reconnues : Ctenocephalides felis strongylus (Jordan, 1925 inféodée au continent africain et Ctenocephalides felis felis (Bouché, 1835 présente dans les zones à climat tempéré (Afrique du Nord, Europe et Amérique (Ménier et Beaucournu, 1999. En Afrique subsaharienne, la principale puce retrouvée chez les animaux de compagnie et chez certains animaux d’élevage (ovins, caprins et bovins appartient à la sous-espèce C. f. strongylus. Quelques paramètres bio-écologiques de C. f. strongylus ont été étudiés dans différentes conditions d’élevage. Les résultats ont été comparés à ceux de C. f. felis actuellement disponibles. À 75% ± 5 d’humidité relative, le cycle de développement de C. f. strongylus dure 20-21 jours à 27 °C et de 16 à 17 jours à 29 °C. Ainsi, la sousespèce africaine de la puce du chat (C. f. strongylus se développe moins vite que C. f. felis à températures identiques. Cette différence pourrait s’expliquer par l’influence du climat de leurs aires de distribution respectives sur leur cycle de développement. À 75% ± 5 d’humidité relative, les adultes de C. f. strongylus ne peuvent survivre plus de 14 jours dans l’environnement à des températures comprises entre 27 et 29 °C, lorsqu’elles n’ont jamais pris de repas sanguin. Dans ces mêmes conditions, la durée de survie n’excède pas 16 jours à 19 °C. Mais lorsque C. f. strongylus a pris un premier repas de sang, elle a une durée de vie beaucoup plus courte lorsqu’elle est hors de son hôte. En effet, aucun individu n’est retrouvé vivant trois jours passé hors de la fourrure de son hôte à 29 °C, cinq jours à 27 °C et huit jours à 19 °C. Il en est de même pour C. f. felis. Ces données sur la bio-écologie de C. f. strongylus permettent de comprendre l’influence de la température sur son cycle de d

  8. High prevalence of Felis catus gammaherpesvirus 1 infection in haemoplasma-infected cats supports co-transmission.

    Science.gov (United States)

    McLuckie, A; Tasker, S; Dhand, N K; Spencer, S; Beatty, J A

    2016-08-01

    Felis catus gammaherpesvirus 1 (FcaGHV1), a potential feline pathogen, has been identified in domestic cats from USA, Asia-Pacific and Central Europe. Transmission of FcaGHV1 during territorial encounters, a route not typical for gammaherpesviruses, is suggested by risk factor analyses from some regions. The aim of this study was to investigate the relationship between FcaGHV1 detection and risk factors, including haemoplasma co-infections, among UK cats to better understand transmission and global distribution of FcaGHV1. FcaGHV1 DNA was detected in blood samples from UK cats (11.56%; 95% confidence interval [CI], 7.47-16.84; n = 199). Logistic regression analyses showed that entire male cats were more likely to be FcaGHV1 positive than neutered male cats (odds ratio, 3.60; 95% CI, 1.22-10.46). Samples positive for DNA from any of three haemoplasma species had 19 times greater odds for testing positive for FcaGHV1 than haemoplasma negative cats in multivariable analyses after adjusting for age, sex and neuter status. Domestic cats in the UK can be infected with FcaGHV1, confirming that this virus is globally endemic. The identification of neuter status as a risk factor for FcaGHV1 detection provides further evidence to support transmission of this virus during territorial encounters and co-transmission with haemoplasmas is suggested. PMID:27387737

  9. What's inside your cat's head? A review of cat (Felis silvestris catus) cognition research past, present and future.

    Science.gov (United States)

    Vitale Shreve, Kristyn R; Udell, Monique A R

    2015-11-01

    The domestic cat (Felis silvestris catus) has shared an intertwined existence with humans for thousands of years, living on our city streets and in our homes. Yet, little scientific research has focused on the cognition of the domestic cat, especially in comparison with human's other companion, the domestic dog (Canis lupus familiaris). This review surveys the current status of several areas of cat cognition research including perception, object permanence, memory, physical causality, quantity and time discrimination, cats' sensitivity to human cues, vocal recognition and communication, attachment bonds, personality, and cognitive health. Although interest in cat cognition is growing, we still have a long way to go until we have an inclusive body of research on the subject. Therefore, this review also identifies areas where future research must be conducted. In addition to the scientific value of future work in this area, future research on cat cognition could have an important influence on the management and welfare of pet and free-roaming cats, leading to improved human-cat interactions. PMID:26154131

  10. Influences of stage of lactation, teat position and sequential milk sampling on the composition of domestic cat milk (Felis catus).

    Science.gov (United States)

    Jacobsen, K L; DePeters, E J; Rogers, Q R; Taylor, S J

    2004-02-01

    Milk from 11 domestic shorthair cats (Felis catus; n=7 fed dry low-fat diet, n=4 fed dry high-fat diet) was collected weekly for 6 weeks following parturition, and analysed for total solids (TS), crude protein (CP), fat, lactose and ash. Samples were collected in 1-ml sequential fractions to determine whether within-sampling changes in composition existed Samples of extracted milk fat were also analysed for fatty acid content. Two commercia kitten milk replacers were analysed according to the same procedures utilized for mil samples. In statistical analyses individual cat, diet, stage of lactation, litter size, and teat position influenced concentrations of milk components; parity and sequential sampling had no effect. Averaged cat milk was 27.9% TS, and 8.7% CP, 12.7% fat, 4.2% lactose and 1.3% ash (on a wet basis). Milk protein percentage increased over lactation for both diet groups, but fat percentage increased only for queens fed the high-fat diet. Milk replacers were lower in fat and protein content than milk from queens, and had considerably lower levels of arachidonic acid. Data from this study contribute to the limited information available regarding the composition of domestic cat milk, and give possible reasons for poor growth occasionally observed in kittens fed unsupplemented commercial milk replacers. PMID:19774762

  11. Multidimensional effects of biologically synthesized silver nanoparticles in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma A549 cells

    Science.gov (United States)

    Gurunathan, Sangiliyandi; Jeong, Jae-Kyo; Han, Jae Woong; Zhang, Xi-Feng; Park, Jung Hyun; Kim, Jin-Hoi

    2015-02-01

    Silver nanoparticles (AgNPs) are prominent group of nanomaterials and are recognized for their diverse applications in various health sectors. This study aimed to synthesize the AgNPs using the leaf extract of Artemisia princeps as a bio-reductant. Furthermore, we evaluated the multidimensional effect of the biologically synthesized AgNPs in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma (A549) cells. UV-visible (UV-vis) spectroscopy confirmed the synthesis of AgNPs. X-ray diffraction (XRD) indicated that the AgNPs are specifically indexed to a crystal structure. The results from Fourier transform infrared spectroscopy (FTIR) indicate that biomolecules are involved in the synthesis and stabilization of AgNPs. Dynamic light scattering (DLS) studies showed the average size distribution of the particle between 10 and 40 nm, and transmission electron microscopy (TEM) confirmed that the AgNPs were significantly well separated and spherical with an average size of 20 nm. AgNPs caused dose-dependent decrease in cell viability and biofilm formation and increase in reactive oxygen species (ROS) generation and DNA fragmentation in H. pylori and H. felis. Furthermore, AgNPs induced mitochondrial-mediated apoptosis in A549 cells; conversely, AgNPs had no significant effects on L132 cells. The results from this study suggest that AgNPs could cause cell-specific apoptosis in mammalian cells. Our findings demonstrate that this environmentally friendly method for the synthesis of AgNPs and that the prepared AgNPs have multidimensional effects such as anti-bacterial and anti-biofilm activity against H. pylori and H. felis and also cytotoxic effects against human cancer cells. This report describes comprehensively the effects of AgNPs on bacteria and mammalian cells. We believe that biologically synthesized AgNPs will open a new avenue towards various biotechnological and biomedical applications in the near future.

  12. Food habits and temporal activity patterns of the Golden Jackal Canis aureus and the Jungle Cat Felis chaos in Pench Tiger Reserve, Madhya Pradesh

    OpenAIRE

    Majumder, A.; K. Sankar; Q. Qureshi; S. Basu

    2011-01-01

    The food habits and temporal activity patterns of the Golden Jackal Canis aureus and the Jungle Cat Felis chaus were studied between January 2008 and June 2009 in Pench Tiger Reserve (PTR), Madhya Pradesh. A total of 50 jackal scats and 85 jungle cat scats were collected where-ever encountered in the study area. Information on activity pattern was obtained using camera traps. Fifty-two pair self-triggered analog cameras were deployed in each 2 x 2 km² across the study area (> 250 km²) close t...

  13. Vocal correlates of sender-identity and arousal in the isolation calls of domestic kitten (Felis silvestris catus

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    Scheumann Marina

    2012-12-01

    Full Text Available Abstract Introduction Human speech does not only communicate linguistic information but also paralinguistic features, e.g. information about the identity and the arousal state of the sender. Comparable morphological and physiological constraints on vocal production in mammals suggest the existence of commonalities encoding sender-identity and the arousal state of a sender across mammals. To explore this hypothesis and to investigate whether specific acoustic parameters encode for sender-identity while others encode for arousal, we studied infants of the domestic cat (Felis silvestris catus. Kittens are an excellent model for analysing vocal correlates of sender-identity and arousal. They strongly depend on the care of their mother. Thus, the acoustical conveyance of sender-identity and arousal may be important for their survival. Results We recorded calls of 18 kittens in an experimentally-induced separation paradigm, where kittens were spatially separated from their mother and siblings. In the Low arousal condition, infants were just separated without any manipulation. In the High arousal condition infants were handled by the experimenter. Multi-parametric sound analyses revealed that kitten isolation calls are individually distinct and differ between the Low and High arousal conditions. Our results suggested that source- and filter-related parameters are important for encoding sender-identity, whereas time-, source- and tonality-related parameters are important for encoding arousal. Conclusion Comparable findings in other mammalian lineages provide evidence for commonalities in non-verbal cues encoding sender-identity and arousal across mammals comparable to paralinguistic cues in humans. This favours the establishment of general concepts for voice recognition and emotions in humans and animals.

  14. Seroprevalence of Rickettsia bellii and Rickettsia felis in dogs, São José dos Pinhais, State of Paraná, Brazil Soroprevalência de Rickettsia bellii e Rickettsia felis em cães, São José dos Pinhais, Paraná, Brasil

    Directory of Open Access Journals (Sweden)

    Fernanda Silva Fortes

    2010-12-01

    Full Text Available Brazilian spotted fever (BSF is a vector-borne zoonosis caused by Rickettsia rickettsii bacteria. Dogs can be host sentinels for this bacterium. The aim of the study was to determine the presence of antibodies against Rickettsia spp. in dogs from the city of São José dos Pinhais, State of Paraná, Southern Brazil, where a human case of BSF was first reported in the state. Between February 2006 and July 2007, serum samples from 364 dogs were collected and tested at 1:64 dilutions by indirect immunofluorescence assay (IFA against R. rickettsii and R. parkeri. All sera that reacted at least to one of Rickettsia species were tested against the six main Rickettsia species identified in Brazil: R. rickettsii, R. parkeri, R. bellii, R. rhipicephali, R. amblyommii and R. felis. Sixteen samples (4.4% reacted to at least one Rickettsia species. Among positive animals, two dogs (15.5% showed suggestive titers for R. bellii exposure. One sample had a homologous reaction to R. felis, a confirmed human pathogen. Although Rickettsia spp. circulation in dogs in the area studied may be considered at low prevalence, suggesting low risk of human infection, the present data demonstrate for the first time the exposure of dogs to R. bellii and R. felis in Southern Brazil.A febre maculosa brasileira (FMB é uma zoonose veiculada por carrapatos e causada pela bactéria Rickettsia rickettsii, podendo os cães ser hospedeiros sentinelas para essa bactéria. O objetivo do estudo foi determinar a presença de anticorpos contra Rickettsia spp. em cães de São José dos Pinhais, estado do Paraná, Sul do Brasil. Entre fevereiro de 2006 e julho de 2007, amostras séricas de 364 cães foram coletadas e testadas na diluição de 1:64 por Reação de Imunofluorescência Indireta (RIFI contra R. rickettsii e R. parkeri. Todos os soros reagentes para pelo menos uma espécie de Rickettsia foram testados contra as seis principais espécies de Rickettsia identificadas no Brasil: R

  15. Avaliação dos estoques pesqueiros de quatro espécies de grandes bagres (Siluriformes, Pimelodidae na bacia do rio Cuiabá, Pantanal norte, Brasil, utilizando alguns Pontos de Referência Biológicos Fisheries stock assessment of four large catfish (Siluriformes, Pimelodidae in the Cuiaba River basin, north Pantanal, Brazil, using some Biological Reference Points

    Directory of Open Access Journals (Sweden)

    Lúcia A. F. Mateus

    2007-03-01

    Full Text Available Os parâmetros de crescimento e mortalidade estimados a partir da estrutura em comprimento foram utilizados para avaliar o estado de explotação dos estoques de quatro espécies de grandes bagres da bacia do rio Cuiabá, Pantanal norte. As espécies estudadas foram o barbado, o cachara, o jaú e o pintado. As avaliações foram realizadas por meio do modelo de rendimento por recruta, considerando os Pontos de Referência Biológicos Fmax, F0,1, F = M e F BDR. As análises indicam que os estoques do pintado e do jaú estão bem conservados. Os estoques do cachara e, menos fortemente, o do barbado estão em iminente ameaça de sobrepesca na bacia do rio Cuiabá.The growth and mortality parameters estimated from length structure were used to evaluate the state of explotation of the stocks of four large catfish species from Cuiabá River basin, north Pantanal. The species studied were the Flatwhiskered catfish, the Jaú, the Barred sorubim, and the Spotted sorubim. The assessment were conducted with the yield per recruit model, and the Biological Reference Points Fmax, F0.1, F = M, and F BDR. The analysis indicates that the stocks of the Spotted sorubim, and of the Jaú are not overexploited. However, the stocks of the Barred sorubim, and, in lower degree, of the Flatwhiskered catfish of the Cuiabá River basin are in imminent threat of overfishing.

  16. Detection of Hepatozoon felis in Ticks Collected from Free-Ranging Amur Tigers ( Panthera tigris altaica), Russian Far East, 2002-12.

    Science.gov (United States)

    Thomas, Lindsay H; Seryodkin, Ivan V; Goodrich, John M; Miquelle, Dale G; Birtles, Richard J; Lewis, John C M

    2016-07-01

    We collected 69 ticks from nine, free-ranging Amur tigers ( Panthera tigris altaica) between 2002 and 2011 and investigated them for tick-borne pathogens. DNA was extracted using alkaline digestion and PCR was performed to detect apicomplexan organisms. Partial 18S rDNA amplification products were obtained from 14 ticks from four tigers, of which 13 yielded unambiguous nucleotide sequence data. Comparative sequence analysis revealed all 13 partial 18S rDNA sequences were most similar to those belonging to strains of Hepatozoon felis (>564/572 base-pair identity, >99% sequence similarity). Although this tick-borne protozoon pathogen has been detected in wild felids from many parts of the world, this is the first record from the Russian Far East.

  17. The detection of Felis catus papillomavirus 3 DNA in a feline bowenoid in situ carcinoma with novel histologic features and benign clinical behavior.

    Science.gov (United States)

    Munday, John S; Fairley, Rob; Atkinson, Karen

    2016-09-01

    Bowenoid in situ carcinoma (BISC; papillomavirus-associated squamous cell carcinoma in situ) is an uncommon skin neoplasm of cats that can result in euthanasia because of the development of multiple lesions or because of progression to invasive squamous cell carcinoma. BISCs are currently thought to be caused by Felis catus papillomavirus 2 (FcaPV-2). The presently described cat developed a single 0.5 cm in diameter interscapular mass. Over the following 18 months, the mass doubled in size; no additional lesions developed. The mass was surgically excised and histologically diagnosed as a BISC. However, in contrast to previously reported BISCs, neither prominent thickening of the deep aspects of the follicular infundibula nor marked cell dysplasia were present. Furthermore, ~50% of the keratinocytes in the affected epidermis had prominent PV cytopathic changes that included shrunken angular nuclei and elongated basophilic cytoplasmic inclusions. As the histopathology was not typical for FcaPV-2 infection, polymerase chain reaction was performed and revealed only DNA sequences from Felis catus papillomavirus 3 (FcaPV-3). No further BISCs developed in this cat 6 months postremoval, hence surgical excision appeared to be curative. Results from this case suggest that, although FcaPV-2 appears to be the predominant cause of BISCs in cats, infection by FcaPV-3 can also cause these neoplasms. BISCs caused by FcaPV-3 appear to have unique histologic features that allow the causative PV type to be predicted. Results from this single case suggest that BISCs caused by FcaPV-3 may have a more benign clinical course than those caused by FcaPV-2. PMID:27423734

  18. Paragonimus y Paragonimiasis en el norte peruano. Proceso del desarrollo de Paragonimus peruvianus Miyazaki¡ Ibañez y Miranda¡ 1969 en Felis cati L. gato doméstico infectado experimentalmente.

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    Nicanor Ibáñez H.

    2014-06-01

    Full Text Available Se ha estudiado el proceso del desarrollo de Parogonimus peruvianus Miyazaki, Ibóñez y Miranda, 1969 en el gato doméstico, Felis cati L. infectado experimentalmente mediante la administración oral de metacercarias del parasito extraídas de ejemplares de Pseudothelphusa chilensis Milne Edwards, 1843 procedentes de áreas endémicas.

  19. Food habits and temporal activity patterns of the Golden Jackal Canis aureus and the Jungle Cat Felis chaos in Pench Tiger Reserve, Madhya Pradesh

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    A. Majumder

    2011-11-01

    Full Text Available The food habits and temporal activity patterns of the Golden Jackal Canis aureus and the Jungle Cat Felis chaus were studied between January 2008 and June 2009 in Pench Tiger Reserve (PTR, Madhya Pradesh. A total of 50 jackal scats and 85 jungle cat scats were collected where-ever encountered in the study area. Information on activity pattern was obtained using camera traps. Fifty-two pair self-triggered analog cameras were deployed in each 2 x 2 km² across the study area (> 250 km² close to animal trails which were set to work on a continuous 24 hour period. Rodents contributed maximum in the diet of these two species (65% golden jackal scats and 56% jungle cat scats. Eight thousand five hundred and sixty camera-trap nights revealed 189 jungle cat captures and 49 golden jackal captures. The activity of golden jackal had a more homogeneous distribution in time. Present study showed that although some degree of overlap is observed between the two sympatric species, an overall difference in dietary composition and activity patterns enabled them to coexist in PTR.

  20. Detection of Felis catus gammaherpesvirus 1 (FcaGHV1) in peripheral blood B- and T-lymphocytes in asymptomatic, naturally-infected domestic cats.

    Science.gov (United States)

    McLuckie, Alicia J; Barrs, Vanessa R; Smith, Adrian L; Beatty, Julia A

    2016-10-01

    The domestic cat is natural host to both feline immunodeficiency virus and Felis catus gammaherpesvirus 1 (FcaGHV1). Comparative data suggest that these agents might act as synergistic copathogens in feline AIDS-related lymphoma. To identify leucocyte subsets harbouring gammaherpesvirus DNA, whole blood from 5 healthy, FcaGHV1-infected cats was labelled with monoclonal antibodies to feline CD21, CD4, CD8 and CD14 for 4-way fluorescence-activated cell sorting. FcaGHV1gB qPCR was performed on DNA extracted from purified fractions and whole blood longitudinally. FcaGHV1 DNA was detected in CD21+, CD4+, CD8+, but not CD14+ cells. Variation in whole blood load, up to 19,788 copies/10(6)cells, was detected in individual cats over time. FcaGHV1 DNA was undetectable in one cat on one occasion highlighting that qPCR of whole blood from a single time point will not detect all cases of FcaGHV1 infection. Further investigation of the role of FcaGHV1 in feline lymphoid malignancies is warranted. PMID:27497183

  1. Complete nucleotide sequences of the domestic cat (Felis catus) mitochondrial genome and a transposed mtDNA tandem repeat (Numt) in the nuclear genome

    Energy Technology Data Exchange (ETDEWEB)

    Lopez, J.V.; Cevario, S.; O`Brien, S.J. [National Cancer Institute, Frederick, MD (United States)

    1996-04-15

    The complete 17,009-bp mitochondrial genome of the domestic cat, Felis catus, has been sequenced and conforms largely to the typical organization of previously characterized mammalian mtDNAs. Codon usage and base composition also followed canonical vertebrate patterns, except for an unusual ATC (non-AUG) codon initiating the NADH dehydrogenase subunit 2 (ND2) gene. Two distinct repetitive motifs at opposite ends of the control region contribute to the relatively large size (1559 bp) of this carnivore mtDNA. Alignment of the feline mtDNA genome to a homologous 7946-bp nuclear mtDNA tandem repeat DNA sequence in the cat, Numt, indicates simple repeat motifs associated with insertion/deletion mutations. Overall DNA sequence divergence between Numt and cytoplasmic mtDNA sequence was only 5.1%. Substitutions predominate at the third codon position of homologous feline protein genes. Phylogenetic analysis of mitochondrial gene sequences confirms the recent transfer of the cytoplasmic mtDNA sequences to the domestic cat nucleus and recapitulates evolutionary relationships between mammal species. 86 refs., 4 figs., 3 tabs.

  2. Transforming properties of Felis catus papillomavirus type 2 E6 and E7 putative oncogenes in vitro and their transcriptional activity in feline squamous cell carcinoma in vivo.

    Science.gov (United States)

    Altamura, Gennaro; Corteggio, Annunziata; Pacini, Laura; Conte, Andrea; Pierantoni, Giovanna Maria; Tommasino, Massimo; Accardi, Rosita; Borzacchiello, Giuseppe

    2016-09-01

    Felis catus papillomavirus type 2 (FcaPV2) DNA is found in feline cutaneous squamous cell carcinomas (SCCs); however, its biological properties are still uncharacterized. In this study, we successfully expressed FcaPV2 E6 and E7 putative oncogenes in feline epithelial cells and demonstrated that FcaPV2 E6 binds to p53, impairing its protein level. In addition, E6 and E7 inhibited ultraviolet B (UVB)-triggered accumulation of p53, p21 and pro-apoptotic markers such as Cleaved Caspase3, Bax and Bak, suggesting a synergistic action of the virus with UV exposure in tumour pathogenesis. Furthermore, FcaPV2 E7 bound to feline pRb and impaired pRb levels, resulting in upregulation of the downstream pro-proliferative genes Cyclin A and Cdc2. Importantly, we demonstrated mRNA expression of FcaPV2 E2, E6 and E7 in feline SCC samples, strengthening the hypothesis of a causative role in the development of feline SCC. PMID:27236740

  3. The glucose and insulin response to isoenergetic reduction of dietary energy sources in a true carnivore: the domestic cat ( Felis catus).

    Science.gov (United States)

    Verbrugghe, Adronie; Hesta, Myriam; Van Weyenberg, Stephanie; Papadopoulos, Georgios A; Gommeren, Kris; Daminet, Sylvie; Bosmans, Tim; Polis, Ingeborgh; Buyse, Johan; Janssens, Geert P J

    2010-07-01

    The present study assessed the effect of separate reduction of each energy-delivering nutrient - protein, fat and carbohydrate - on glucose tolerance and insulin response in a strict carnivore: the domestic cat (Felis catus). Three isoenergetic, home-made diets with the following energetic distribution, low protein (LP): protein 28 % of metabolisable energy; fat 43 %; nitrogen-free extract 29 %; low fat: 47, 27 and 25 %; low carbohydrate (LC): 45, 48 and 7 %, were tested in a 3 x 3 Latin square design. Nine healthy normal-weight cats were randomly assigned to each of the diets in a random order at intervals of 3 weeks. At the end of each testing period, intravenous glucose tolerance tests were performed. Plasma glucose concentrations and area under the glucose curve showed no differences. Area under the insulin curve was lower when cats were fed the LP diet, and the second insulin peak tended to be delayed when the LC diet was fed. In contrast to other studies, in which energy sources were elevated instead of being reduced, the present trial contradicts the often suggested negative impact of carbohydrates on insulin sensitivity in carnivores, and shows that reducing the dietary carbohydrate content below common amounts for commercial foods evokes an insulin-resistant state, which can be explained by the cats' strict carnivorous nature. It even points to a negative effect of protein on insulin sensitivity, a finding that corresponds with the highly gluconeogenic nature of amino acids in strict carnivores. PMID:20193098

  4. The biological and reproductive parameters of the invasive armored catfish Pterygoplichthys disjunctivus from Adolfo López Mateos El Infiernillo Reservoir, Michoacán-Guerrero, Mexico Parámetros biológicos y reproductivos del bagre armado invasivo Pterygoplichthys disjunctivus del embalse Adolfo López Mateos El Infiernillo, Michoacán-Guerrero, México

    Directory of Open Access Journals (Sweden)

    Rebeca Aneli Rueda-Jasso

    2013-03-01

    Full Text Available Invasive Pterygoplichthys disjunctivus (Weber, 1991 (synonymy: Liposarcus multiradiatus armored catfish were first reported in Mexico in 1995 and have spread successfully in several aquatic ecosystems. In Adolfo López Mateos El Infiernillo Reservoir, Michoacán - Guerrero, the population of this armored catfish is growing substantially because natural predators are not present and exploitation by humans does not occur. Over a complete annual cycle, we studied the following population parameters: weight, total and standard length, the relationship of weight to total length, the hepatosomatic (HSI and gonadosomatic (GSI indices, total fecundity (TF and relative fecundity (RF. The average total (TL and standard lengths (SL were 248 ± 37.5 and 188 ± 30.7 mm, respectively, and the average weight (W was 135.3± 66.8 g. The largest fish reported was 520 mm in TL and weighed 1 280 g. The species showed an extended reproductive season from May to November, as indicated by the GSI, TF and RF. The strongest reproductive peak occurred from July through October. During the annual cycle, the highest monthly average fecundity was 2 447 eggs, and the average relative fecundity was 12.6 eggs per g of fish (August and September. This information will be crucial for calculating the current biomass and future growth of the population. The calculation of these results could provide a basis for the exploitation of this resource for human consumption and animal feeds.La especie invasiva "bagre armado" P. disjunctivus (Weber 1991 (sinonimia de Liposarcus multuradiatus fue registrada por primera vez en México en 1995; en diversos sistemas acuáticos se ha distribuido exitosamente. En la presa Adolfo López Mateos El Infiernillo, Michoacán - Guerrero, la población de peces armados se ha incrementado considerablemente debido a la ausencia de depredadores y la falta de aprovechamiento. Por ello, a lo largo de un ciclo anual evaluamos los siguientes par

  5. Dietary canitine maintains energy reserves and delays fatigue of exercised african catfish (Clarias gariepinus fed high fat diets Carnitina dietética mantem reservas energéticas e evita a fatiga de bagre-africano durante exercício

    Directory of Open Access Journals (Sweden)

    Rodrigo Ozório

    2005-06-01

    Full Text Available Lipids, together with proteins, are traditionally considered as primary fuels during aerobic swimming. The effects of dietary fat and carnitine supplements and exercise on the energy metabolism of juvenile fish were investigated. One hundred African catfish (Clarias gariepinus were fed four isonitrogenous diets containing a fat level of 100 or 190 g kg-1 diet and one of the two levels of carnitine (15 and 1000 mg kg-1. Fish grew from 61 to 162 g in 10 wk. Thereafter, 6 fish per group swam vigorously for 3 h and the results were compared with unexercised groups. Fish receiving 1,000 mg carnitine accumulated 2- to 3-fold more carnitine than fish receiving 15 mg carnitine. Plasma acyl-carnitine level was affected by an interaction between dietary treatment and exercise (P Lipídios e proteínas são tradicionalmente considerados combustíveis primários durante natação aeróbica. Nesse ensaio foi investigado o efeito da suplementação de vários níveis de gordura e carnitina no metabolismo de 100 bagres africanos juvenis (Clarias gariepinus. Os peixes foram arraçoados com quatro dietas isoprotéicas, cada uma contendo 100 ou 190 g gordura kg-1 dieta, e um dos dois níveis de carnitina (15 e 1000 mg kg-1. Os peixes cresceram de 61 a 162 g em 10 semanas. No final do ensaio de alimentação, grupos de seis peixes por tratamento foram induzidos a nadar vigorosamente por 3 h e em seguida vários parâmetros foram determinados no tecido muscular e plasma, e os resultados observados nos grupos exercitados foram comparados com grupos controles (não exercitados. Os peixes arraçoados com 1,000 mg carnitina acumularam de duas a três vezes mais carnitina que os peixes arraçoados com 15 mg carnitina. O nível de acyl-carnitina no plasma foi influenciado pela interação entre os tratamentos dietéticos e exercício físico (P < 0.05. As concentrações de adenosina trifosfato (ATP e fosfocreatina no tecido muscular branco (WM foram mais elevadas em

  6. Absence of zoonotic Bartonella species in questing ticks: First detection of Bartonella clarridgeiae and Rickettsia felis in cat fleas in the Netherlands

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    Reimerink Johan R

    2011-04-01

    Full Text Available Abstract Background Awareness for flea- and tick-borne infections has grown in recent years and the range of microorganisms associated with these ectoparasites is rising. Bartonella henselae, the causative agent of Cat Scratch Disease, and other Bartonella species have been reported in fleas and ticks. The role of Ixodes ricinus ticks in the natural cycle of Bartonella spp. and the transmission of these bacteria to humans is unclear. Rickettsia spp. have also been reported from as well ticks as also from fleas. However, to date no flea-borne Rickettsia spp. were reported from the Netherlands. Here, the presence of Bartonellaceae and Rickettsiae in ectoparasites was investigated using molecular detection and identification on part of the gltA- and 16S rRNA-genes. Results The zoonotic Bartonella clarridgeiae and Rickettsia felis were detected for the first time in Dutch cat fleas. B. henselae was found in cat fleas and B. schoenbuchensis in ticks and keds feeding on deer. Two Bartonella species, previously identified in rodents, were found in wild mice and their fleas. However, none of these microorganisms were found in 1719 questing Ixodes ricinus ticks. Notably, the gltA gene amplified from DNA lysates of approximately 10% of the questing nymph and adult ticks was similar to that of an uncultured Bartonella-related species found in other hard tick species. The gltA gene of this Bartonella-related species was also detected in questing larvae for which a 16S rRNA gene PCR also tested positive for "Candidatus Midichloria mitochondrii". The gltA-gene of the Bartonella-related species found in I. ricinus may therefore be from this endosymbiont. Conclusions We conclude that the risk of acquiring Cat Scratch Disease or a related bartonellosis from questing ticks in the Netherlands is negligible. On the other hand fleas and deer keds are probable vectors for associated Bartonella species between animals and might also transmit Bartonella spp. to humans.

  7. Spatio-temporal variation in predation by urban domestic cats (Felis catus and the acceptability of possible management actions in the UK.

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    Rebecca L Thomas

    Full Text Available Urban domestic cat (Felis catus populations can attain exceedingly high densities and are not limited by natural prey availability. This has generated concerns that they may negatively affect prey populations, leading to calls for management. We enlisted cat-owners to record prey returned home to estimate patterns of predation by free-roaming pets in different localities within the town of Reading, UK and questionnaire surveys were used to quantify attitudes to different possible management strategies. Prey return rates were highly variable: only 20% of cats returned ≥4 dead prey annually. Consequently, approximately 65% of owners received no prey in a given season, but this declined to 22% after eight seasons. The estimated mean predation rate was 18.3 prey cat⁻¹ year⁻¹ but this varied markedly both spatially and temporally: per capita predation rates declined with increasing cat density. Comparisons with estimates of the density of six common bird prey species indicated that cats killed numbers equivalent to adult density on c. 39% of occasions. Population modeling studies suggest that such predation rates could significantly reduce the size of local bird populations for common urban species. Conversely, most urban residents did not consider cat predation to be a significant problem. Collar-mounted anti-predation devices were the only management action acceptable to the majority of urban residents (65%, but were less acceptable to cat-owners because of perceived risks to their pets; only 24% of cats were fitted with such devices. Overall, cat predation did appear to be of sufficient magnitude to affect some prey populations, although further investigation of some key aspects of cat predation is warranted. Management of the predation behavior of urban cat populations in the UK is likely to be challenging and achieving this would require considerable engagement with cat owners.

  8. Seroprevalence of Toxoplasma gondii in White-Tailed Deer (Odocoileus virginianus) and Free-Roaming Cats (Felis catus) Across a Suburban to Urban Gradient in Northeastern Ohio.

    Science.gov (United States)

    Ballash, Gregory A; Dubey, J P; Kwok, O C H; Shoben, Abigail B; Robison, Terry L; Kraft, Tom J; Dennis, Patricia M

    2015-06-01

    Felids serve as the definitive host of Toxoplasma gondii contaminating environments with oocysts. White-tailed deer (WTD; Odocoileus virginianus) are used as sentinel species for contaminated environments as well as a potential source for human foodborne infection with T. gondii. Here we determine the seroprevalence of T. gondii in a WTD and felid population, and examine those risk factors that increase exposure to the parasite. Serum samples from 444 WTD and 200 free-roaming cats (Felis catus) from urban and suburban reservations were tested for T. gondii antibodies using the modified agglutination test (MAT, cut-off 1:25). Antibodies to T. gondii were found in 261 (58.8%) of 444 WTD, with 164 (66.1%) of 248 from urban and 97 (49.5%) of 196 from suburban regions. Significant risk factors for seroprevalence included increasing age (P < 0.0001), reservation type (P < 0.0001), and household densities within reservation (P < 0.0001). Antibodies to T. gondii were found in 103 (51.5%) of 200 cats, with seroprevalences of 79 (51%) of 155 and 24 (53.3%) of 45 from areas surrounding urban and suburban reservations, respectively. Seroprevalence did not differ by age, gender, or reservation among the cats' sample. Results indicate WTD are exposed by horizontal transmission, and this occurs more frequently in urban environments. The difference between urban and suburban cat densities is the most likely the reason for an increased seroprevalence in urban WTD. These data have public health implications for individuals living near or visiting urban areas where outdoor cats are abundant as well as those individuals who may consume WTD venison. PMID:25269422

  9. Feeding habits of Barbul de piedra (ariopsis sp.) in the Sinu River, Colombia

    International Nuclear Information System (INIS)

    The feeding habits of Barbul de piedra in the Sinu River were studied. the stomach content was analyzed using the proportion of empty stomachs, grade of digestion, frequency of occurrence, numerical frequency, gravimetry, relative importance index and the gut length total length relationship. Only 18.8% of stomachs were empty, most of preys were half digested and five food groups were identified: crustaceans, fishes, vegetable rests, molluscs and others. Crustaceans were the most frequent, abundant and greater weight composition group. The relative importance index indicated that crustaceans and fishes were secondary relative importance groups and vegetable rests, molluscs and others were occasional or circumstantial groups. The results achieved in this work indicate that Barbul de piedra is a fish that keeps your feeding habits as they grow and along the hydrological cycle, although shows carnivorous preference, especially by crustaceans.

  10. Synthèse et étude structrale de lyonsite-type (Na0,4,Li0,6(Fe,Li2(MoO43

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    Amira Souilem

    2015-06-01

    Full Text Available The new compound (Na0.4,Li0.6(Fe,Li2(MoO43 was synthesized by cooling from the melt. Its anionic framework is built up from two distinct MO6 octahedra, each containing disordered Li+ and Fe3+ ions in 0.6:0.4 and 0.7:0.3 ratios, and two MoO4 tetrahedra, which link by vertex-sharing of their O atoms. These tetrameric units are further linked by sharing edges between octahedra and by formation of M—O—Mo (M = Fe/Li bridges, forming ribbons propagating in the [100] direction. The ribbons are cross-linked in both the b- and c-axis directions, giving rise to a three-dimensional framework having [100] tunnels in which the monovalent Na+/Li+ cations (0.4:0.6 ratio lie. Bond-valence calculations are consistent with the disorder model for the cations. The structure of the title compound, which is isotypic with Li3Fe(MoO43 and Li3Ga(MoO43, is compared briefly with those of LiFeMo2O8 and Li1.6Mn2.2(MoO43.

  11. Spotlight census of the red fox (Vulpes vulpes and the domestic cat (Felis catus in three sample areas of the Marches region (Central Italy / Censimento notturno di Volpe (Vulpes vulpes e di Gatto domestico (Felis catus in tre aree campione delle Marche

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    Massimo Pandolfi

    1991-07-01

    Full Text Available Abstract In order to evaluate the density of the red fox and of the domestic cat, 55 transects were made from 1986 to 1989 using spotlight census method in three sample areas. The mean density of foxes agreed substantially with its biological cycle and the hightes values (2.01 foxes/km² in spring and 4.3 foxes/km² in winter were recorded in the study area with the better natural characteristics. Foxes selected the shrub woodland (macchia all year round, the inhabited area in spring. The domestic cat was widely spread and abundant, and selected especially inhabited areas where the density varied from 4.27 cats/km² (in winter to 12.42 cat/km² (in spring. Riassunto Dal 1986 al 1989, con il metodo dei percorsi notturni con fari, sono stati effettuati complessivamente 55 conteggi in tre aree campione per valutare la densità della Volpe (Vulpes vulpes e del Gatto domestico (Felis catus nonché le loro preferenze ambientali limitatamente ad una zona campione. Per la Volpe le densità medie rilevate sono sostanzialmente in accordo con il ciclo biologico della specie e quelle più elevate (2,O1 volpi/km² in primavera e 4,3 volpi/km² in inverno sono state registrate nella zona campione con maggior presenza di boschi ed aree incolte. La Volpe seleziona le zone con vegetazione "di macchia" in ogni periodo dell'anno, e le aree abitate in primavera. Per il Gatto domestico le densità rilevate evidenziano la presenza di una diffusa ed abbondante popolazione. La specie mostra una spiccata preferenza per le aree abitate dove raggiunge densità di 4,27 individui/km² e 12,42 individui/km² in inverno e primavera rispettivamente.

  12. Comparative efficacy on dogs of a single topical treatment with fipronil/(S)-methoprene or weekly physiological hygiene shampoos against Ctenocephalides felis in a simulated flea-infested environment.

    Science.gov (United States)

    Beugnet, F; Fourie, J; Chalvet-Monfray, K

    2012-05-01

    Flea infestations of pets continue to persist due to the lack of knowledge of flea biology and ecology. It is not unusual that pet owners believe regular hygiene, such as shampooing their dogs can replace regular insecticidal treatment. The objective of this study was to compare in a flea simulated environment, modelling exposure similar to that found in a home, that the use of regular physiological shampoo does not control fleas adequately when compared to a long acting topical formulation. Three groups of six dogs were formed: one untreated control group, one group treated monthly with the topical formulation of fipronil/(S)-methoprene, and a third group treated weekly with a hygiene shampoo. All dogs were infested with adult unfed Ctenocephalides felis fleas (200±5) on Days -28 and -21. Each animal's sleeping box was fitted with a plastic cup mounted to the inside roof of the box. The sleeping bench of each animal was covered with a carpet to accommodate flea development. The dogs were maintained in their kennels throughout the study. In order to maintain the environmental flea challenge, C. felis pupae (100±5) were placed in the plastic cup in each animal's sleeping box on Days -14, -7, 0, 7, 14, 21, 28, 35 and 42. The dogs were combed and fleas counted weekly on Days -1, 3, 10, 17, 24, 31, 38, 45, and 51. The fleas were placed immediately back on the dogs. On Day 60, fleas were counted and removed. Flea infestations in the untreated control group at each count averaged between 46.2 and 74.2 fleas throughout the study. The average number of fleas infesting dogs was significantly different (pshampoo group was never above 79.2%. Weekly shampooing in treatment group 3 was intentionally delayed after Day 42, to evaluate wether missing a weekly bath would affect the flea population. The Day 48 data indicate that forgetting or delaying a single weekly shampooing resulted in a clear increase in flea numbers and a significant decrease in efficacy from 68.2% to 34

  13. Comparative efficacy on dogs of a single topical treatment with fipronil/(S)-methoprene or weekly physiological hygiene shampoos against Ctenocephalides felis in a simulated flea-infested environment.

    Science.gov (United States)

    Beugnet, F; Fourie, J; Chalvet-Monfray, K

    2012-05-01

    Flea infestations of pets continue to persist due to the lack of knowledge of flea biology and ecology. It is not unusual that pet owners believe regular hygiene, such as shampooing their dogs can replace regular insecticidal treatment. The objective of this study was to compare in a flea simulated environment, modelling exposure similar to that found in a home, that the use of regular physiological shampoo does not control fleas adequately when compared to a long acting topical formulation. Three groups of six dogs were formed: one untreated control group, one group treated monthly with the topical formulation of fipronil/(S)-methoprene, and a third group treated weekly with a hygiene shampoo. All dogs were infested with adult unfed Ctenocephalides felis fleas (200±5) on Days -28 and -21. Each animal's sleeping box was fitted with a plastic cup mounted to the inside roof of the box. The sleeping bench of each animal was covered with a carpet to accommodate flea development. The dogs were maintained in their kennels throughout the study. In order to maintain the environmental flea challenge, C. felis pupae (100±5) were placed in the plastic cup in each animal's sleeping box on Days -14, -7, 0, 7, 14, 21, 28, 35 and 42. The dogs were combed and fleas counted weekly on Days -1, 3, 10, 17, 24, 31, 38, 45, and 51. The fleas were placed immediately back on the dogs. On Day 60, fleas were counted and removed. Flea infestations in the untreated control group at each count averaged between 46.2 and 74.2 fleas throughout the study. The average number of fleas infesting dogs was significantly different (pshampoo group was never above 79.2%. Weekly shampooing in treatment group 3 was intentionally delayed after Day 42, to evaluate wether missing a weekly bath would affect the flea population. The Day 48 data indicate that forgetting or delaying a single weekly shampooing resulted in a clear increase in flea numbers and a significant decrease in efficacy from 68.2% to 34

  14. Comparative efficacy on dogs of a single topical treatment with fipronil/(S-methoprene or weekly physiological hygiene shampoos against Ctenocephalides felis in a simulated flea-infested environment

    Directory of Open Access Journals (Sweden)

    Beugnet F.

    2012-05-01

    Full Text Available Flea infestations of pets continue to persist due to the lack of knowledge of flea biology and ecology. It is not unusual that pet owners believe regular hygiene, such as shampooing their dogs can replace regular insecticidal treatment. The objective of this study was to compare in a flea simulated environment, modelling exposure similar to that found in a home, that the use of regular physiological shampoo does not control fleas adequately when compared to a long acting topical formulation. Three groups of six dogs were formed: one untreated control group, one group treated monthly with the topical formulation of fipronil/(S-methoprene, and a third group treated weekly with a hygiene shampoo. All dogs were infested with adult unfed Ctenocephalides felis fleas (200 ± 5 on Days -28 and -21. Each animal’s sleeping box was fitted with a plastic cup mounted to the inside roof of the box. The sleeping bench of each animal was covered with a carpet to accommodate flea development. The dogs were maintained in their kennels throughout the study. In order to maintain the environmental flea challenge, C. felis pupae (100 ± 5 were placed in the plastic cup in each animal’s sleeping box on Days -14, -7, 0, 7, 14, 21, 28, 35 and 42. The dogs were combed and fleas counted weekly on Days -1, 3, 10, 17, 24, 31, 38, 45, and 51. The fleas were placed immediately back on the dogs. On Day 60, fleas were counted and removed. Flea infestations in the untreated control group at each count averaged between 46.2 and 74.2 fleas throughout the study. The average number of fleas infesting dogs was significantly different (p < 0.05 between the untreated and the two treatment groups and between the two treatment groups at all counts throughout the two months study (Days 3, 10, 17, 24, 31, 38, 45, 51 and 60. The efficacy was never below 99.1% in the fipronil/(S-methoprene group, and efficacy in the shampoo group was never above 79.2%. Weekly shampooing in treatment

  15. Toxoplasmosis in Sand cats (Felis margarita) and other animals in the Breeding Centre for Endangered Arabian Wildlife in the United Arab Emirates and Al Wabra Wildlife Preservation, the State of Qatar.

    Science.gov (United States)

    Dubey, J P; Pas, An; Rajendran, C; Kwok, O C H; Ferreira, L R; Martins, J; Hebel, C; Hammer, S; Su, C

    2010-09-20

    The Sand cat (Felis margarita) is a small-sized felid found in sand and stone deserts ranging from the north of Africa to Asia, with the Arabian Peninsula as its centre of distribution. The Sand cat captive breeding program at the Breeding Centre for Endangered Arabian Wildlife (BCEAW), Sharjah, UAE, has experienced high newborn mortality rates, and congenital toxoplasmosis was recently recognized as one of the causes of this mortality. In the present study, one 18-month-old Sand cat (FM019) died of acute toxoplasmosis-associated hepatitis and pneumonitis acquired after birth; Toxoplasma gondii was demonstrated in histological sections which reacted with T. gondii polyclonal antibodies by immunohistochemistry (IHC). T. gondii DNA was found by PCR of extracted DNA from liver and lung tissues of this cat. Antibodies to T. gondii were found in serum examined in 1:1600 dilution in the modified agglutination test (MAT); its 2-year-old cage mate seroconverted (MAT titer 1:3200) at the same time. Another Sand cat (FM017) was euthanized because of ill health when 3 years old; its MAT titer was >1:3200, and T. gondii tissue cysts were found in brain, heart, ocular muscles and skeletal muscle, confirmed by IHC. Viable T. gondii was isolated by bioassays in mice inoculated with tissues of another chronically infected Sand cat (FM002); T. gondii was not found in histological sections of this cat. T. gondii antibodies were found in several species of animals tested, notably in 49 of 57 wild felids at BCEAW. A 7-year-old Sand cat (3657) from Al Wabra Wildlife Preservation (AWWP), Doha, State of Qatar died of acute visceral toxoplasmosis with demonstrable T. gondii tachyzoites by IHC, and T. gondii DNA by PCR, and a MAT titer of >3200. T. gondii antibodies were found in 21 of 27 of wild felids at AWWP. PCR-RFLP genotyping at 10 genetic loci revealed that these T. gondii isolates from Sand cat (FM002 and FM019) at BCEAW have an atypical genotype, which was previously reported in T

  16. Toxoplasmosis in Sand cats (Felis margarita) and other animals in the Breeding Centre for Endangered Arabian Wildlife in the United Arab Emirates and Al Wabra Wildlife Preservation, the State of Qatar.

    Science.gov (United States)

    Dubey, J P; Pas, An; Rajendran, C; Kwok, O C H; Ferreira, L R; Martins, J; Hebel, C; Hammer, S; Su, C

    2010-09-20

    The Sand cat (Felis margarita) is a small-sized felid found in sand and stone deserts ranging from the north of Africa to Asia, with the Arabian Peninsula as its centre of distribution. The Sand cat captive breeding program at the Breeding Centre for Endangered Arabian Wildlife (BCEAW), Sharjah, UAE, has experienced high newborn mortality rates, and congenital toxoplasmosis was recently recognized as one of the causes of this mortality. In the present study, one 18-month-old Sand cat (FM019) died of acute toxoplasmosis-associated hepatitis and pneumonitis acquired after birth; Toxoplasma gondii was demonstrated in histological sections which reacted with T. gondii polyclonal antibodies by immunohistochemistry (IHC). T. gondii DNA was found by PCR of extracted DNA from liver and lung tissues of this cat. Antibodies to T. gondii were found in serum examined in 1:1600 dilution in the modified agglutination test (MAT); its 2-year-old cage mate seroconverted (MAT titer 1:3200) at the same time. Another Sand cat (FM017) was euthanized because of ill health when 3 years old; its MAT titer was >1:3200, and T. gondii tissue cysts were found in brain, heart, ocular muscles and skeletal muscle, confirmed by IHC. Viable T. gondii was isolated by bioassays in mice inoculated with tissues of another chronically infected Sand cat (FM002); T. gondii was not found in histological sections of this cat. T. gondii antibodies were found in several species of animals tested, notably in 49 of 57 wild felids at BCEAW. A 7-year-old Sand cat (3657) from Al Wabra Wildlife Preservation (AWWP), Doha, State of Qatar died of acute visceral toxoplasmosis with demonstrable T. gondii tachyzoites by IHC, and T. gondii DNA by PCR, and a MAT titer of >3200. T. gondii antibodies were found in 21 of 27 of wild felids at AWWP. PCR-RFLP genotyping at 10 genetic loci revealed that these T. gondii isolates from Sand cat (FM002 and FM019) at BCEAW have an atypical genotype, which was previously reported in T

  17. Folliculogenesis in the domestic cat (Felis catus).

    Science.gov (United States)

    Bristol-Gould, Sarah; Woodruff, Teresa K

    2006-07-01

    The dynamic regulation of mammalian folliculogenesis is a key component of the reproductive process. Traditionally, the rodent had been used as a model to study ovarian function and reproductive physiology due to the availability of animals, their relatively short cycle length, high rate of fecundity and short generation interval. We maintain that much basic information can be determined using domestic cat ovaries retrieved from local veterinary clinics following routine spaying, without having the expense of maintaining a colony of laboratory cats. Studies of normal feline reproductive physiology and advances in reproductive technology may be extrapolated for use in endangered non-domestic felids. Increased understanding of feline reproduction will be beneficial to veterinary medicine, and to groups working to control feral cat populations. It is important to examine reproductive mechanisms in alternative animal models as there are a vast number of threatened and endangered species in which we lack the critical reproductive information needed to assist in preserving their long-term survival. PMID:16620931

  18. Estudo microscópico e macroscópico, com enfoque radiográfico e de alizarina, no desenvolvimento embrionário e fetal de gatos domésticos (Felis catus em diferentes idades gestacionais

    Directory of Open Access Journals (Sweden)

    Dilayla K Abreu

    2011-12-01

    Full Text Available O gato doméstico (Felis catus foi nomeado por Carolus Linnaeus em seu livro Systema Naturae, em 1798. A família Felidea apresenta muita semelhança morfológica com os felinos selvagens. O estudo da embriologia do gato doméstico é de grande valia, uma vez que, é considerado um importante modelo animal quando comparado aos gatos selvagem em extinção, especialmente relacionado às pesquisas sobre biologia reprodutiva. Este trabalho objetivou análisar e comparar as fases embrionárias de quatro embriões e um feto de felinos domésticos. Nos embriões com idade gestacional estimada em 17 dias (0,5cm CR podemos observar pela análise macroscópica a presença de dilatação rostral correspondente ao prosencéfalo, o local placóide do cristalino, a flexura cervical, os quatro arcos faríngeos com os sulcos que o dividem, a proeminência cardíaca, o indício do brotamento do membro pélvico, além da presença de somitos. Na região caudal do embrião, visualizamos a curvatura cranio-caudal, permitindo ao mesmo uma posição em formato de "C". Nos embriões com idade gestacional estimada em 22 dias (1,2cm CR, na análise macroscópica foi visualizado o prosencéfalo, vesícula óptica com pigmentação da retina, vesícula ótica, quarto ventrículo, fígado, membros torácicos e pélvicos com discreta distinção dos dígitos e vascularização superficial. Nos embriões com idade gestacional estimada em 25 dias (1,5cm CR notamos a presença do prosencéfalo e mesencéfalo, a curvatura cervical pronunciada, vesícula óptica com forte pigmentação da retina, vesícula ótica, membros pélvicos e torácicos bem desenvolvidos, com distinção dos dígitos e fígado bem pronunciado. Os fetos com idade gestacional estimada em 52 dias (10cm CR possuem estruturas internas e externas facilmente identificadas em animais adultos. Com relação às estruturas ósseas notamos que as mesmas não apresentam nenhuma epífise óssea formada, sendo vis

  19. Assessing Introgression betweenEuropean Wildcats (Felis silvestris silvestris) and Domestic Cats (Felis silvestris catus)

    OpenAIRE

    Nussberger, Beatrice

    2013-01-01

    Introgression is an important issue in evolutionarybiology. It is defined as the flow of genes between taxa through hybridization beyond the first generation. Introduced genes of a closely related taxon may serve as raw material for rapid adaptive evolutionary change. On the other hand, introgression could lead to reduced fitness in hybrids, i.e. outbreeding depression, if the newly mixed traits are maladapted to the environment or if, on the genomiclevel, co-adapted gene complexes are di...

  20. Pseudocapillaria yucatanensis sp. n. (Nematoda: Capillariidae) from the bagre Rhamdia guatemalensis (Pisces) from cenotes in Yucatan, Mexico.

    Science.gov (United States)

    Moravec, F; Scholz, T; Vivas Rodríguez, C

    1995-01-01

    A new nematode species, Pseudocapillaria yucatanensis sp. n., is described from the intestine of the freshwater pimelodid catfish Rhamdia guatemalensis (Günther) from cenotes (= sinkholes) in Yucatan, Mexico. It differs from other three related species parasitizing freshwater fishes mainly in possessing the spicule with a simple rim of its proximal end and a non-expanded distal end, in the length of the spicule (0.218-0.295 mm), and the size (0.050-0.060 x 0.025-0.030 mm), shape and structure of eggs, and also in the host types and geographical distribution. Pseudocapillaria yucatanensis is the first known autochtonous species of Pseudocapillaria parasitizing freshwater fishes in Mexico. PMID:9599428

  1. Amino acid specificity of fibers of the facial/trigeminal complex innervating the maxillary barbel in the Japanese sea catfish, Plotosus japonicus.

    Science.gov (United States)

    Caprio, John; Shimohara, Mami; Marui, Takayuki; Kohbara, Jun; Harada, Shuitsu; Kiyohara, Sadao

    2015-12-01

    The Japanese sea catfish, Plotosus japonicus, possesses taste and solitary chemoreceptor cells (SCCs) located on the external body surface that detect specific water-soluble substances. Here, we identify two major fiber types of the facial/trigeminal complex that transmit amino acid information to the medulla. Both single and few fiber preparations respond to amino acid stimulation in the 0.1 μM to mM range. One fiber type responds best to glycine and l-alanine (i.e. Gly/Ala fibers) whereas the other fiber type is best stimulated by l-proline and glycine betaine (hereafter referred to only as betaine) (i.e. Pro/Bet fibers). We demonstrate that betaine, which does not alter the pH of the seawater and therefore does not activate the animals' highly sensitive pH sensors (Caprio et al., Science 344:1154-1156, 2014), is sufficient to elicit appetitive food search behavior. We further show that the amino acid specificity of fibers of the facial/trigeminal complex in P. japonicus is different from that in Ariopsis felis (Michel and Caprio, J. Neurophysiol. 66:247-260, 1991; Michel et al., J. Comp. Physiol. A. 172:129-138, 1993), a representative member of the only other family (Ariidae) of extant marine catfishes.

  2. Fecal endocrine profiles and ejaculate traits in black-footed cats (Felis nigripes) and sand cats (Felis margarita).

    Science.gov (United States)

    Herrick, J R; Bond, J B; Campbell, M; Levens, G; Moore, T; Benson, K; D'Agostino, J; West, G; Okeson, D M; Coke, R; Portacio, S C; Leiske, K; Kreider, C; Polumbo, P J; Swanson, W F

    2010-01-15

    Information regarding the reproductive biology of black-footed cats (BFC) and sand cats (SC) is extremely limited. Our objectives were to: (1) validate fecal hormone analysis (estrogens, E; progestagens, P; androgens, T) for noninvasive monitoring of gonadal activity; (2) characterize estrous cyclicity, ovulatory mechanisms, gestation, and seasonality; and (3) evaluate male reproductive activity via fecal androgen metabolites and ejaculate traits. In both species, the estrous cycle averaged 11-12 days. In BFC (n=8), estrus lasted 2.2+/-0.2 days with peak concentrations of E (2962.8+/-166.3 ng/g feces) increasing 2.7-fold above basal concentrations. In SC (n=6), peak concentrations of E (1669.9+/-83.5 ng/g feces) during estrus (2.9+/-0.2 days) were 4.0-fold higher than basal concentrations. Nonpregnant luteal phases occurred in 26.5% (26 of 98) of BFC estrous cycles, but were not observed in SC (0 of 109 cycles). In both species, P concentrations during pregnancy were elevated (32.3+/-3.0 microg/g feces BFC; 8.5+/-0.7 microg/g feces SC) approximately 10-fold above basal concentrations. Fecal T concentrations in males averaged 3.1+/-0.1 microg/g feces in BFC and 2.3+/-0.0 microg/g feces in SC. Following electroejaculation, 200 to 250 microl of semen was collected containing 29.9 (BFC) to 36.5 (SC)x10(6) spermatozoa with 40.4 (SC) to 46.8 (BFC)% normal morphology. All females exhibited estrous cycles during the study and spermatozoa were recovered from all males on every collection attempt, suggesting poor reproductive success in these species may not be due to physiological infertility.

  3. Predator cue and prey density interactively influence indirect effects on basal resources in intertidal oyster reefs.

    Directory of Open Access Journals (Sweden)

    A Randall Hughes

    Full Text Available Predators can influence prey abundance and traits by direct consumption, as well as by non-consumptive effects of visual, olfactory, or tactile cues. The strength of these non-consumptive effects (NCEs can be influenced by a variety of factors, including predator foraging mode, temporal variation in predator cues, and the density of competing prey. Testing the relative importance of these factors for determining NCEs is critical to our understanding of predator-prey interactions in a variety of settings. We addressed this knowledge gap by conducting two mesocosm experiments in a tri-trophic intertidal oyster reef food web. More specifically, we tested how a predatory fish (hardhead catfish, Ariopsis felis directly influenced their prey (mud crabs, Panopeus spp. and indirectly affected basal resources (juvenile oysters, Crassostrea virginica, as well as whether these direct and indirect effects changed across a density gradient of competing prey. Per capita crab foraging rates were inversely influenced by crab density, but they were not affected by water-borne predator cues. As a result, direct consumptive effects on prey foraging rates were stronger than non-consumptive effects. In contrast, predator cue and crab density interactively influenced indirect predator effects on oyster mortality in two experiments, with trait-mediated and density-mediated effects of similar magnitude operating to enhance oyster abundance. Consistent differences between a variable predator cue environment and other predator cue treatments (no cue and constant cue suggests that an understanding of the natural risk environment experienced by prey is critical to testing and interpreting trait-mediated indirect interactions. Further, the prey response to the risk environment may be highly dependent on prey density, particularly in prey populations with strong intra-specific interactions.

  4. Spatial variability of primary organic sources regulates ichthyofauna distribution despite seasonal influence in Terminos lagoon and continental shelf of Campeche, Mexico

    Science.gov (United States)

    Romo Rios, J. A.; Aguíñiga-García, S.; Sanchez, A.; Zetina-Rejón, M.; Arreguín-Sánchez, F.; Tripp-Valdéz, A.; Galeana-Cortazár, A.

    2013-05-01

    Human activities have strong impacts on coastal ecosystems functioning through their effect on primary organic sources distributions and resulting biodiversity. Hence, it appears to be of utmost importance to quantify contribution of primary producers to sediment organic matter (SOM) spatial variability and its associated ichthyofauna. The Terminos lagoon (Gulf of Mexico) is a tropical estuary severely impacted by human activities even though of primary concern for its biodiversity, its habitats, and its resource supply. Stable isotope data (d13C, d15N) from mangrove, seaweed, seagrass, phytoplankton, ichthyofauna and SOM were sampled in four zones of the lagoon and the continental shelf through windy (November to February), dry (March to June) and rainy (July to October) seasons. Stable Isotope Analysis in R (SIAR) mixing model were used to determine relative contributions of the autotrophic sources to the ichthyofauna and SOM. Analysis of variance of ichthyofauna isotopic values showed significant differences (P < 0.001) in the four zones of lagoon despite the variability introduced by the windy, dry and rainy seasons. In lagoons rivers discharge zone, the mangrove contribution to ichthyofauna was 40% and 84% to SOM. Alternative use of habitat by ichthyofauna was evidenced since in the deep area of the lagoon (4 m), the contribution of mangrove to fish is 50%, and meanwhile contribution to SOM is only 77%. Although phytoplankton (43%) and seaweed (41%) contributions to the adjacent continental shelf ichthyofauna were the main organic sources, there was 37% mangrove contribution to SOM, demonstrating conspicuous terrigenous influence from lagoon ecosystem. Our results point toward organic sources spatial variations that regulate fish distribution. In Terminos lagoon, significant correlation (p-value = 0.2141 and r=0.79) of Ariopsis felis and Sphoeroides testudineus abundances and seaweed and seagrasses contributions (30-35%) during both dry and rainy seasons

  5. Esporotricose do gato doméstico (Felis catus: transmissão humana Sporothricosis of the domestic cat (Felis catus: human transmission

    Directory of Open Access Journals (Sweden)

    Silvio Alencar Marques

    1993-08-01

    Full Text Available No presente trabalho relata-se caso de paciente, funcionário de hospital veterinário, infectado através de arranhadura de gato doméstico portador de esporotricose. Inquérito domiciliar junto aos proprietários do animal fonte de infecção, revelou dois outros casos presuntivos de esporotricose humana transmitida por gatos, e confirmou o diagnóstico, por cultivo do Sporotrix schenckii, em 3 gatos domésticos adicionais. A esporotricose felina caracteriza-se por lesões cutâneas ulceradas e tendência à disseminação sistêmica e evolução fatal. A transmissão intra e inter-espécie é facilitada pela exuberância de fungos nas lesões cutâneas de felinos infectados.A case of sporothricosis transmitted by cat to a veterinarian hospital employee is reported. Inquiry at domiciliar area of the cat's owner revelled two other presumable cases of human sporothricosis transmitted by cats, and confirmed the diagnosis (by culture of Sporothrix schenckii of disease in three other domestic cats. Feline sporothricosis is characterized by ulcerative, cutaneous lesions and systemic dissemination, which invariably cause animal's death. The transmission of sporothricosis to other animals and humans is enhanced by the great amount of fungus present in cat's lesions.

  6. Isolation and genetic characterization of Toxoplasma gondii from black bears (Ursus americanus), bobcats (Felis rufus), and feral cats (Felis catus) from Pennsylvania

    Science.gov (United States)

    Toxoplasma gondii infects virtually all warm-blooded hosts worldwide. Recently, attention has been focused on the genetic diversity of the parasite to explain its pathogenicity in different hosts. It has been hypothesized that interaction between feral and domestic cycles of T. gondii may increase u...

  7. Biología de la gestación en la gata doméstica (Felis catus Biology of pregnancy in the domestic cat (Felis catus

    Directory of Open Access Journals (Sweden)

    A.E SANCHEZ

    2002-01-01

    Full Text Available La gata es una hembra poliéstrica estacional en la cual el estímulo coital desencadena la liberación de LH y la ovulación, fenómenos que ocurren dentro de las primeras 50 horas postcoito. La fecundación de los ovocitos ocurre en el oviducto dentro de 30 horas post-ovulación. El transporte embrionario en oviducto toma alrededor de 132 horas y al momento de ingresar al útero los embriones se encuentran al estado de mórula compacta. A continuación los blastocistos migran entre los cuernos uterinos por aproximadamente 80 horas, hasta producirse la implantación 12 a 13 días post-coito. Durante la etapa preimplantacional existe un aumento significativo de la progesterona sérica así como de los receptores luteales a LH. A partir de la segunda mitad de la gestación, decae la producción de progesterona y aumenta la secreción de prolactina, postulándose que esta última sería el principal agente luteotrófico en la gata. También durante la segunda mitad de la gestación aumenta la secreción de relaxina. La producción y rol de la progesterona en la gestación tardía es un tema controversial. Se ha demostrado que la placenta felina posee actividad esteroidogénica y capacidad de sintetizar progesterona, lo cual sugiere que estaría relacionada con el soporte hormonal de la preñezThe domestic cat can be defined as a seasonal poliestrous female. During mating the physical stimulus produce the release of LH and ovulation, which occur during the first 50 hours post mating. The fertilization of oocytes takes place inside the oviduct during the 30 hours post ovulation. The embryos transport on the oviduct takes about 132 hours and when embryos reach the uterus they are already a compacted morulae. After this, the blastocyst migrates to both uterine horns for a period of 80 hours aproximately until implantation ocurrs 12 to 13 days after mating. During the preimplantation period, there is a significant increase in blood progesterone and also in LH lutheal receptors. From the second half of gestation, the production of progesterone decrease and prolactin secretion increase. Thus it is thought that the later would be the main luteotrophic agent in the cat. Also during the second half of gestation the secretion of relaxin increase. The production and function of progesterone during late gestation is controversial. It has been shown that placenta of cats has a steroidogenic function and that it is also to produce progesterone

  8. Rickettsia felis Infection in a Common Household Insect Pest, Liposcelis bostrychophila (Psocoptera: Liposcelidae)▿ †

    OpenAIRE

    Behar, Adi; McCormick, Laurie J.; Perlman, Steve J.

    2010-01-01

    Many species of Rickettsia are well-known mammalian pathogens transmitted by blood-feeding arthropods. However, molecular surveys are continually uncovering novel Rickettsia species, often in unexpected hosts, including many arthropods that do not feed on blood. This study reports a systematic molecular characterization of a Rickettsia infecting the psocid Liposcelis bostrychophila (Psocoptera: Liposcelidae), a common and cosmopolitan household pest. Surprisingly, the psocid Rickettsia is sho...

  9. Seroprevalence of Toxoplasma gondii in Cats (Felis catus, Linnaeus 1758 Living in San Carlos (Chile

    Directory of Open Access Journals (Sweden)

    Ignacio Eduardo Troncoso Toro

    2015-05-01

    Full Text Available There are few studies about seroprevalence of toxoplasmosis in Chile; therefore, this article aims to determine seroprevalence in cats in the district of San Carlos, by ELISA Immuno- Comb® serological technique, and, at the same time, to examine association with variables of sex, age, diet, and habitat. To the effect, 60 cats over 2 months old were randomly sampled. Sera were analyzed using the ELISA ImmunoComb® Biogal Toxo & Chlamydia test kit, which detects specific immunoglobulin G-type antibodies against Toxoplasma gondii with a sensitivity of 92.3% and a specificity of 100%. The study evidenced that 29 individuals were positive (48.3% seroprevalence; when broken down by gender this corresponded to 9 males and 20 females (39.1% and 54%, respectively. By age, seropositivity was higher in the “Adult” group (76.7%, followed by groups “Over 7 years” (50% and “Young” (25%. With respect to diet, higher seropositivity was obtained in animals fed on mixed diet, as opposed to commercial diet (60% vs. 47.2%. By variable habitat, 16 indoor and 13 outdoor cats were positive (45.7% and 52%, showing statistically significant difference only for the variable age (p < 0.05. Finally, through relating age with seropositivity, a negative correlation was evidenced (r = –0.3, indicating that older individuals had lower seroprevalence. The results show the presence of antibodies against Toxoplasma gondii in domestic cats.

  10. Seasonal Variation in the Voluntary Food Intake of Domesticated Cats (Felis Catus)

    OpenAIRE

    Serisier, Samuel; Feugier, Alexandre; Delmotte, Sebastien; Biourge, Vincent; German, Alexander James

    2014-01-01

    There are numerous reports about seasonal cycles on food intake in animals but information is limited in dogs and cats. A 4-year prospective, observational, cohort study was conducted to assess differences in food intake in 38 ad-libitum-fed adult colony cats, of various breeds, ages and genders. Individual food intake was recorded on a daily basis, and the mean daily intake for each calendar month was calculated. These data were compared with climatic data (temperature and daylight length) f...

  11. Flat Feline Faces: Is Brachycephaly Associated with Respiratory Abnormalities in the Domestic Cat (Felis catus)?

    Science.gov (United States)

    Packer, Rowena M. A.; Caney, Sarah M. A.; Gunn-Moore, Danièlle A.

    2016-01-01

    There has been little research into brachycephalism and associated disorders in cats. A questionnaire aimed at cat owners was used to determine the relationship between feline facial conformation and owner-reported cat management requirements and respiratory abnormalities. Owner-submitted photographs of cats were used to develop novel measures of skull conformation. One thousand valid questionnaires were received. Within these there were 373 valid photographs that allowed measurement of muzzle ratio (M%) and 494 that allowed nose position ratio (NP%). The data included 239 cats for which both measurements were available. Owners reported lifestyle factors (e.g. feeding type, grooming routine, activity level), physical characteristics (e.g. hair length) and other health characteristics of their cat (e.g. tear staining, body condition score). A composite respiratory score (RS) was calculated for each cat using their owner’s assessment of respiratory noise whilst their cat was asleep and then breathing difficulty following activity. Multivariate analyses were carried out using linear models to explore the relationship between RS and facial conformation, and lifestyle risk factors. The results showed that reductions in NP% and M% were significantly associated with RS (P < 0.001 and P = 0.026, respectively) and that the relationship was significantly negatively correlated (r = -0.56, P < 0.001 for both). Respiratory score was also significantly associated with increased presence of tear staining (P < 0.001) and a sedentary lifestyle (P = 0.01). This study improves current knowledge concerning cats with breeding-related alterations in skull confirmation and indicates that brachycephalism may have negative respiratory implications for cat health and welfare, as has been previously shown in dogs. PMID:27574987

  12. Computed tomography (CT) of the granial mediastinum in the cat (Felis silverstris F. domestica)

    International Nuclear Information System (INIS)

    14 mature clinically healthy domestic cats were studied using contrast computed tomography. Tomographic slices were made transversally across second (Th2) and third (Th3) thoracic vertebrae. The study was carried out using a soft-tissue and lung window. When using a lung window (Th2) the mediastinum was visualised as a heterodense mass, delineated by the lungs. The use of a soft-tissue window at levels Th2 and Th3 visualised the main mediastinal vessels and allowed a percise anatomic CT analysis

  13. Growth of a male Caracal kitten felis Caracal in the Mountain Zebra National Park

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    J. H Grobler

    1982-11-01

    Full Text Available Three caracal (lynx Fells caracal kittens, two females and a male were received from the Fish River area some 50 km north of Cradock, Republic of South Africa, on the 20th March 1980. These were found at the base of a dense Rhus erosa bush on the side of a hill and estimated at 14 days old based on the findings of Cade (1968 Int. Zoo Yb. 45 and Kralik (1967 Int. Zoo Yb. 132.

  14. Detection of antibodies against Leishmania infantum in cats (Felis catus from the State of Pernambuco, Brazil

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    Rita de Cássia Nascimento Silva

    2014-01-01

    Full Text Available Introduction: Little information is available concerning infection by Leishmania infantum in cats. Therefore, the aim of this study was to perform a serological study in domestic cats. Methods: Serum samples (n=153 obtained from animals living in the Cities of Recife and Petrolina, State of Pernambuco, Brazil, were tested by ELISA/S7® (Biogene. Results: Anti-L. infantum antibodies were detected in 3.9% (6/153 of the cats. All seroreagent animals were from Petrolina. Conclusions: These results serve as an important alert, and future studies are needed to better understand the possible role of cats in the epidemiology of visceral leishmaniasis (VL in this area.

  15. Domestic Cats (Felis silvestris catus Do Not Show Signs of Secure Attachment to Their Owners.

    Directory of Open Access Journals (Sweden)

    Alice Potter

    Full Text Available The Ainsworth Strange Situation Test (SST has been widely used to demonstrate that the bond between both children and dogs to their primary carer typically meets the requirements of a secure attachment (i.e. the carer being perceived as a focus of safety and security in otherwise threatening environments, and has been adapted for cats with a similar claim made. However methodological problems in this latter research make the claim that the cat-owner bond is typically a secure attachment, operationally definable by its behaviour in the SST, questionable. We therefore developed an adapted version of the SST with the necessary methodological controls which include a full counterbalance of the procedure. A cross-over design experiment with 20 cat-owner pairs (10 each undertaking one of the two versions of the SST first and continuous focal sampling was used to record the duration of a range of behavioural states expressed by the cats that might be useful for assessing secure attachment. Since data were not normally distributed, non-parametric analyses were used on those behaviours shown to be reliable across the two versions of the test (which excluded much cat behaviour. Although cats vocalised more when the owner rather the stranger left the cat with the other individual, there was no other evidence consistent with the interpretation of the bond between a cat and its owner meeting the requirements of a secure attachment. These results are consistent with the view that adult cats are typically quite autonomous, even in their social relationships, and not necessarily dependent on others to provide a sense of security and safety. It is concluded that alternative methods need to be developed to characterise the normal psychological features of the cat-owner bond.

  16. Retroviruses and sexual size dimorphism in domestic cats (Felis catus L.).

    OpenAIRE

    Pontier, D; Fromont, E; Courchamp, F.; Artois, M; Yoccoz, N G

    1998-01-01

    Hochberg and co-workers have predicted that an increase in host adult mortality due to parasites is balanced by an earlier age at first reproduction. In polygynous species we hypothesize that such a pattern would lead to diverging selection pressure on body size between sexes and increased sexual size dimorphism. In polygynous mammals, male body size is considered to be an important factor for reproductive success. Thus, under the pressure of a virulent infection, males should be selected for...

  17. Individual differences in visual and olfactory cue preference and use by cats (Felis catus)

    OpenAIRE

    Mayes, Evely.-Rose E.; Wilkinson, Anna; Pike, Thomas W; Mills, Daniel S.

    2015-01-01

    Highlights • Eight cats were trained in a T-maze using a two-alternative forced choice procedure. • Cats could use either an olfactory or visual cue to locate a food reward. • Cues were then put in conflict to determine which was preferred for the task. • Most cats used the visual cue to learn the location of the food. • Preferences were stable, repeatable and rapidly learned. -------------------------------------------------------------------------------- ...

  18. Effect of melatonin implants on spermatogenesis in the domestic cat (Felis silvestris catus).

    Science.gov (United States)

    Favre, R Nuñez; Bonaura, M C; Praderio, R; Stornelli, M C; de la Sota, R L; Stornelli, M A

    2014-10-01

    The aim of this study was to assess the efficacy of subcutaneous melatonin implants to temporarily and reversibly suppress spermatogenesis in male cats. Tomcats (n = 8) were housed in a conditioned room with alternating long and short 2-month photoperiod cycles to maintain sperm production and quality. Animals were randomly assigned to one of the two treatments. Four animals received a subcutaneous melatonin implant (MEL, 18 mg; Syntex, Argentina), whereas the other four received a subcutaneous placebo implant (PLA, 0 mg; Syntex). Semen samples were collected by electroejaculation every 14 days for 252 days. Sperm parameters were evaluated in all ejaculates, and data were analyzed by ANOVA. Melatonin-implanted cats significantly decreased their sperm quality in all the parameters studied compared with the control group (MEL vs. PLA; least squares means ± SEM; motility, 71.3 ± 3.4 vs. 82.1 ± 3.6; velocity, 3.4 ± 0.1 vs. 4.6 ± 0.1; total sperm count, 2.6 ± 2.2 vs. 19.4 ± 3.3; acrosome integrity, 48.7 ± 5.6 vs. 62.8 ± 5.6; plasma membrane integrity, 52.2 ± 4.7 vs. 72.9 ± 5.5; normal sperm morphology, 45.8 ± 3.3 vs. 63.7 ± 3.4; P 0.05). At 91 ± 7 days after implant insertion, sperm motility decreased 38.5%, velocity 26.5%, total sperm count 82%, acrosome integrity 22%, plasma membrane integrity 30%, and normal sperm morphology decreased 32% of preimplant values. This effect was present until 120 ± 15 days after implant insertion. After that, seminal parameters started to increase and reached preimplant values at about 140 ± 7 days after implant insertion. Nevertheless, treated animals conserved the capacity to produce semen during the treatment period. In conclusion, a single subcutaneous melatonin implant effectively and reversibly reduced sperm production and quality in male domestic cats for approximately 120 ± 15 days without clinically detectable adverse effects. PMID:25082022

  19. [Architectural structure of the pterygoidian plane of the masticatory musculature in the cat (Felis catus L.)].

    Science.gov (United States)

    Lautrou, A; Laison, F

    1975-09-01

    The architectural analysis of the ptérygoidal plane of the cat identified the two pterygoid muscles. The medial pterygoid showed an orbital and an angular part inserted respectively on the internal face of the mandibule and on the maeto-angular ligament. The lateral pterygoid consisted of only one pterygoid bundle, involved in lateral movements and adjustments of the jaw condyle.

  20. Estudo anatômico do trajeto do canal mandibular em felinos (Felis catus domesticus

    Directory of Open Access Journals (Sweden)

    S.C. Cotrim

    2015-12-01

    Full Text Available Objetivou-se descrever, por meio de tomografia computadorizada, o trajeto do canal mandibular (CM em 20 gatos sem raça definida, com ausência de alterações na cavidade oral, provenientes do Centro de Controle de Zoonoses do Distrito Federal. Foram realizados cortes tomográficos com 2mm de espessura, acompanhando todo o trajeto do CM, tendo como referência a região do forame mandibular, as raízes distais e mesiais dos dentes pré-molares e molares e o forame mentoniano, obtendo-se medidas desde o CM até as faces vestibular, lingual, ventral e alveolar (profundidade do corpo da mandíbula, bem como seu diâmetro. Pôde constatar que o CM manteve-se no aspecto lingual do corpo da mandíbula desde o forame mandibular até a raiz mesial do 1º pré-molar, onde se deslocou para a face vestibular, emergindo no forame mentoniano. Com relação à profundidade, seu trajeto sofreu declive a partir do forame mandibular até a região da raiz mesial do 1º molar, onde alcançou seu ponto mais profundo para prosseguir em suave ascensão até o forame mentoniano. Os dados apresentados contribuem para o estudo anatômico da mandíbula de gatos, bem como auxiliam no melhor planejamento e execução de procedimentos cirúrgicos na mandíbula dessa espécie.

  1. Função testicular em gatos domésticos (Felis catus)

    OpenAIRE

    Meyer, Katlyn Barp

    2013-01-01

    Resumo: O presente trabalho teve como objetivo avaliar a esteroidogênese e a espermatogênese em relação à qualidade espermática em machos de gatos domésticos de forma a responder questões sobre a sazonalidade reprodutiva e o fenômeno da teratospermia em felinos. Foram realizadas análises da morfologia e de parâmetros físicos de espermatozóides obtidos da cauda do epidídimo, mensuração das concentrações sérica de testosterona (T) e intratesticulares de T e 17?-estradiol (E2) e análise da ativi...

  2. Estacionalidad reproductiva en animales domésticos : Nuevas perspectivas en el gato (Felis silvestris catus)

    OpenAIRE

    Núñez Favre, Romina de los Ángeles; Bonaura, María Candela; García Mitacek, María Carla; Stornelli, María Cecilia; Stornelli, María Alejandra; Sota, Rodolfo Luzbel de la

    2013-01-01

    Algunas especies de animales como los pequeños rumiantes y algunos roedores presentan un período de reposo sexual estacional de duración e intensidad variable. Esta estacionalidad está directamente relacionada con las horas luz diarias (fotoperiodo) a las que se hallan sometidos los animales y se evidencia en las localizaciones geográficas en las que existen marcadas variaciones en la duración del día durante el año. En el caso de los pequeños rumiantes, ambos sexos tienen una actividad sexua...

  3. Flat Feline Faces: Is Brachycephaly Associated with Respiratory Abnormalities in the Domestic Cat (Felis catus)?

    Science.gov (United States)

    Farnworth, Mark J; Chen, Ruoning; Packer, Rowena M A; Caney, Sarah M A; Gunn-Moore, Danièlle A

    2016-01-01

    There has been little research into brachycephalism and associated disorders in cats. A questionnaire aimed at cat owners was used to determine the relationship between feline facial conformation and owner-reported cat management requirements and respiratory abnormalities. Owner-submitted photographs of cats were used to develop novel measures of skull conformation. One thousand valid questionnaires were received. Within these there were 373 valid photographs that allowed measurement of muzzle ratio (M%) and 494 that allowed nose position ratio (NP%). The data included 239 cats for which both measurements were available. Owners reported lifestyle factors (e.g. feeding type, grooming routine, activity level), physical characteristics (e.g. hair length) and other health characteristics of their cat (e.g. tear staining, body condition score). A composite respiratory score (RS) was calculated for each cat using their owner's assessment of respiratory noise whilst their cat was asleep and then breathing difficulty following activity. Multivariate analyses were carried out using linear models to explore the relationship between RS and facial conformation, and lifestyle risk factors. The results showed that reductions in NP% and M% were significantly associated with RS (P < 0.001 and P = 0.026, respectively) and that the relationship was significantly negatively correlated (r = -0.56, P < 0.001 for both). Respiratory score was also significantly associated with increased presence of tear staining (P < 0.001) and a sedentary lifestyle (P = 0.01). This study improves current knowledge concerning cats with breeding-related alterations in skull confirmation and indicates that brachycephalism may have negative respiratory implications for cat health and welfare, as has been previously shown in dogs. PMID:27574987

  4. Serotyping of Toxoplasma gondii in Cats (Felis domesticus) Reveals Predominance of Type II Infections in Germany

    Science.gov (United States)

    Background: Cats are definitive hosts of Toxoplasma gondii and play an essential role in the epidemiology of this parasite. The study aims at clarifying whether cats are able to develop specific antibodies against different clonal types of T. gondii and to determine by serotyping the T. gondii clona...

  5. Evaluation of cats (Felis catus) as possible asymptomatic carriers of dermatophytes in extreme south of Brazil

    Institute of Scientific and Technical Information of China (English)

    Tony Silveira; Renata de Faria; Mariana Remio; Camila Graeff; Fabiana Poetsch; Guilherme Azevedo; Juliane Guimares; Rafaela Bellora; Tassiane Moraes; Pedro Quevedo

    2015-01-01

    Objective: To investigate the presence of Microsporum canis in pelage of asymptomatic cats for dermatophytosis, in south region of Rio Grande do Sul, Brazil, and evaluate its importance in epidemiology of dermatophytosis in the study area. Methods: A total of 60 domestic cats were evaluated for the presence of Microsporum canis. The animals were divided into three groups of 20 felines. Each group consisted of exclusively domiciled, semi-domiciled and rural animals. Samples were collected following the carpet-square technique. The microorganisms were cultivated under laminar flow in mycosel agar and grown in a greenhouse. Results: All the cats of the three groups analysed had negative cultures for dermatophytes. In 85% of the dishes, there was a growth of environmental saprophytic fungi such as Aspergillus sp., Fusarium sp. and Penicillium sp. Conclusions: Thus, asymptomatic cats for dermatophytes did not show importance in the transmission and maintenance of the disease in southern of Rio Grande do Sul, Brazil.

  6. Coat genetic markers of the domestic cat Felis catus (Felidae from southwestern Colombia

    Directory of Open Access Journals (Sweden)

    Mauricio Peñuela A

    2016-05-01

    Full Text Available Objectives. Establish the genetic profiles of cats from 12 neighboring municipalities in southwestern Colombia, in a town course from Pereira-Popayán. Estimate the degree of diversity, genetic structure, and quantify gene flow. Materials and methods. Were inventoried the phenotypic markers present in the pigmentation and structure of the coat of 1482 cats of the municipalities surveyed. Based on these phenotypic frequencies, allele frequencies, heterozygosity, Hardy-Weinberg equilibrium, F statistics and Nei genetic distances were calculated. A comparison was also made between genetic and geographic distance matrices to determine if there was a significant association between the two. Results. With the genetic profiles of the populations we estimated the degree of diversity. We found the populations in equilibrium for the S autosomal locus and for the O sex-linked locus. We found a low level genetic structure, and it was determined that there was no significant correlation between the genetic and geographic distance matrices among populations. Conclusions: These findings can be explained on the basis of the processes of human displacement for this region, due to the fact that the establishment of feline populations in these municipalities originated during the same historical period. Identical genetic profiles are shared as a result of colonization events, and due to possible continued migration among these populations.

  7. Seasonal Variation in the Voluntary Food Intake of Domesticated Cats (Felis Catus)

    OpenAIRE

    Samuel Serisier; Alexandre Feugier; Sébastien Delmotte; Vincent Biourge; Alexander James German

    2014-01-01

    International audience There are numerous reports about seasonal cycles on food intake in animals but information is limited in dogs and cats. A 4-year prospective, observational, cohort study was conducted to assess differences in food intake in 38 ad-libitum-fed adult colony cats, of various breeds, ages and genders. Individual food intake was recorded on a daily basis, and the mean daily intake for each calendar month was calculated. These data were compared with climatic data (temperat...

  8. A Mutation in LTBP2 Causes Congenital Glaucoma in Domestic Cats (Felis catus).

    Science.gov (United States)

    Kuehn, Markus H; Lipsett, Koren A; Menotti-Raymond, Marilyn; Whitmore, S Scott; Scheetz, Todd E; David, Victor A; O'Brien, Stephen J; Zhao, Zhongyuan; Jens, Jackie K; Snella, Elizabeth M; Ellinwood, N Matthew; McLellan, Gillian J

    2016-01-01

    The glaucomas are a group of diseases characterized by optic nerve damage that together represent a leading cause of blindness in the human population and in domestic animals. Here we report a mutation in LTBP2 that causes primary congenital glaucoma (PCG) in domestic cats. We identified a spontaneous form of PCG in cats and established a breeding colony segregating for PCG consistent with fully penetrant, autosomal recessive inheritance of the trait. Elevated intraocular pressure, globe enlargement and elongated ciliary processes were consistently observed in all affected cats by 8 weeks of age. Varying degrees of optic nerve damage resulted by 6 months of age. Although subtle lens zonular instability was a common feature in this cohort, pronounced ectopia lentis was identified in less than 10% of cats examined. Thus, glaucoma in this pedigree is attributed to histologically confirmed arrest in the early post-natal development of the aqueous humor outflow pathways in the anterior segment of the eyes of affected animals. Using a candidate gene approach, significant linkage was established on cat chromosome B3 (LOD 18.38, θ = 0.00) using tightly linked short tandem repeat (STR) loci to the candidate gene, LTBP2. A 4 base-pair insertion was identified in exon 8 of LTBP2 in affected individuals that generates a frame shift that completely alters the downstream open reading frame and eliminates functional domains. Thus, we describe the first spontaneous and highly penetrant non-rodent model of PCG identifying a valuable animal model for primary glaucoma that closely resembles the human disease, providing valuable insights into mechanisms underlying the disease and a valuable animal model for testing therapies. PMID:27149523

  9. Domestic cats (Felis catus) are definitive hosts for Sarcocystis sinensis from water buffaloes (Bubalus bubalis).

    Science.gov (United States)

    Gjerde, Bjørn; Hilali, Mosaad

    2016-08-01

    The definitive hosts of Sarcocystis sinensis in water buffaloes have hitherto been unknown, but the close similarity of this species to the cat-transmitted Sarcocystis bovifelis in cattle suggested they were felids. In a previous study, two domestic cats were fed macroscopic sarcocysts of Sarcocystis fusiformis contained within or dissected from the esophageal muscles of water buffaloes, while no microscopic sarcocysts of S. sinensis were noticed. Both cats started shedding small numbers of sporocysts 8-10 days post infection (dpi) and were euthanized 15 dpi. Using a PCR-based molecular assay targeting the mitochondrial cox1 gene of S. fusiformis, both cats were shown to act as definitive hosts for this species. In the present study, DNA samples derived from oocysts/sporocysts in the intestinal mucosa of both cats were further examined by PCR for the presence of S. sinensis using 2 newly designed primers selectively targeting the cox1 gene of this species. All 6 DNA samples examined from each cat tested positive for S. sinensis. A 1,038-bp-long portion of cox1 was amplified and sequenced as 2 overlapping fragments from 5 of these DNA samples. The 5 sequences shared 99.3-100% identity with 7 previous cox1 sequences of S. sinensis obtained from sarcocysts in water buffaloes. Additionally, amplification of the ITS1 region with primers targeting various Sarcocystis spp., yielded amplicons of 2 different lengths, corresponding to those obtained from sarcocyst isolates of S. sinensis and S. fusiformis, respectively. This is the first study to show that cats act as definitive hosts for S. sinensis. PMID:27075117

  10. An STR forensic typing system for genetic individualization of domestic cat (Felis catus) samples.

    Science.gov (United States)

    Menotti-Raymond, Marilyn A; David, Victor A; Wachter, Leslie L; Butler, John M; O'Brien, Stephen J

    2005-09-01

    A forensic genotyping panel of 11 tetranucleotide STR loci from the domestic cat was characterized and evaluated for genetic individualization of cat tissues. We first examined 49 candidate STR loci and their frequency assessment in domestic cat populations. The STR loci (3-4 base pair repeat motifs), mapped in the cat genome relative to 579 coding loci and 255 STR loci, are well distributed across the 18 feline autosomes. All loci exhibit Mendelian inheritance in a multi-generation pedigree. Eleven loci that were unlinked and were highly heterozygous in cat breeds were selected for a forensic panel. Heterozygosity values obtained for the independent loci, ranged from 0.60-0.82, while the average cat breed heterozygosity obtained for the 11 locus panel was 0.71 (range of 0.57-0.83). A small sample set of outbred domestic cats displayed a heterozygosity of 0.86 for the 11 locus panel. The power of discrimination of the panel is moderate to high in the cat breeds examined, with an average P(m) of 3.7E-06. The panel shows good potential for genetic individualization within outbred domestic cats with a P(m) of 5.31E-08. A multiplex protocol, designed for the co-amplification of the 11 loci and a gender-identifying locus, is species specific and robust, generating a product profile with as little as 0.125 nanograms of genomic DNA.

  11. Physical Map and Genome Sequencing Survey of Mycoplasma haemofelis (Haemobartonella felis)

    OpenAIRE

    Berent, Linda M.; Joanne B. Messick

    2003-01-01

    Mycoplasma haemofelis is an uncultivable red-cell pathogen of cats. Isolated M. haemofelis DNA was used to create a bacterial artificial chromosome library and physical map. Random sequencing of this material revealed 75 genes that had not been previously reported for M. haemofelis or any other hemotrophic mycoplasma.

  12. A Mutation in LTBP2 Causes Congenital Glaucoma in Domestic Cats (Felis catus).

    Science.gov (United States)

    Kuehn, Markus H; Lipsett, Koren A; Menotti-Raymond, Marilyn; Whitmore, S Scott; Scheetz, Todd E; David, Victor A; O'Brien, Stephen J; Zhao, Zhongyuan; Jens, Jackie K; Snella, Elizabeth M; Ellinwood, N Matthew; McLellan, Gillian J

    2016-01-01

    The glaucomas are a group of diseases characterized by optic nerve damage that together represent a leading cause of blindness in the human population and in domestic animals. Here we report a mutation in LTBP2 that causes primary congenital glaucoma (PCG) in domestic cats. We identified a spontaneous form of PCG in cats and established a breeding colony segregating for PCG consistent with fully penetrant, autosomal recessive inheritance of the trait. Elevated intraocular pressure, globe enlargement and elongated ciliary processes were consistently observed in all affected cats by 8 weeks of age. Varying degrees of optic nerve damage resulted by 6 months of age. Although subtle lens zonular instability was a common feature in this cohort, pronounced ectopia lentis was identified in less than 10% of cats examined. Thus, glaucoma in this pedigree is attributed to histologically confirmed arrest in the early post-natal development of the aqueous humor outflow pathways in the anterior segment of the eyes of affected animals. Using a candidate gene approach, significant linkage was established on cat chromosome B3 (LOD 18.38, θ = 0.00) using tightly linked short tandem repeat (STR) loci to the candidate gene, LTBP2. A 4 base-pair insertion was identified in exon 8 of LTBP2 in affected individuals that generates a frame shift that completely alters the downstream open reading frame and eliminates functional domains. Thus, we describe the first spontaneous and highly penetrant non-rodent model of PCG identifying a valuable animal model for primary glaucoma that closely resembles the human disease, providing valuable insights into mechanisms underlying the disease and a valuable animal model for testing therapies.

  13. Distribuição, abundância relativa e movimentos sazonais de bagres marinhos (Siluriformes, Ariidae no estuário da Lagoa dos Patos (RS, Brasil

    Directory of Open Access Journals (Sweden)

    Francisco Gerson Araújo

    1988-01-01

    Full Text Available Three ariid fish, Netuma barba, Netuma planifrons and Genidens genidens, use the estuary of the Patos Lagoon (32ºS, Rio Grande do Sul, Brazil, as a nursery and feeding ground in various stages of their life history. A comparative study was made of the temporal and spatial distribution and relative abundance. Seasonal movements were interpreted on the basis of distribution and abundance of the young of these coexisting sea catfishe. Monthly otter trawl samples were made between November, 1979 and April, 1983. Temperature, salinities and dissolved oxygen contents were taken at each of the 674 sample stations. All three species were caught in ample temperature (12,1 to 26,3ºC, salinity (0,3 to 35,5‰ and dissolved oxygen (4,7 to 11,5 mg/l ranges. However, they were more abundant in stations of low temperature and in salinity with highly dissolved oxygen content. N. barba was the most abundant catfish caught in the estuary, it constituted 81,9% in number and 81,4% in weight of ali ariid trawl catches during the study. N. barba occurred throughout the estuary, from the adjacent coastal region to the upper reaches of the Patos Lagoon estuary near the liminetic zone. N. planifrons occurred from adjacent coastal regions to low saline parts of the Patos Lagoon estuary, whereas G. genidens were distributed mainly in low saline and freshwater parts of the lagoon. Young-of-the-year N. barba aggregated near of the entrance of the lagoon in fall and winter, then moved up to intermediary parts of the estuary in spring, and they went to the upper part of the estuary in summer. Yearlings N. barba moved into the estuary in common with young-of-the-year but did not reach the coastal adjacent area. Young-of-the-year and yearling N. planifrons moved in to the estuary as the same pattern as N. barba but they became scarce during the late summer and early fall. G. genidens were scarcely captured in the estuary throughout the year. The spatial and temporal segregations among Netuma and Genidens were evident. A hypothetical model for the life history of N. barba is suggested.

  14. Descripción de una nueva especie de bagre marino fósil (Teleostei, Siluriformes, Ariidae del Mioceno de la provincia de Río Negro, Argentina

    Directory of Open Access Journals (Sweden)

    Sergio Bogan

    2011-01-01

    Full Text Available En este trabajo se describen dos cráneos de la familia Ariidae procedentes del miembro Saladar de la Formación Gran Bajo del Gualicho, ubicados en las Salinas del Gualicho, provincia de Río Negro, Argentina. La edad de los depósitos del Miembro Saladar se remonta al Mioceno Temprano alto/Mioceno Medio bajo. Los materiales son asignados a una nueva especie extinta del género viviente Genidens. Este nuevo taxón se distingue de todas las especies actuales por presentar un proceso supraoccipital muy corto, ancho y redondeado, extraescapular subcircular y mesetmoides amplios con una conspicua escotadura mesial, frontales prácticamente planos en vista lateral y margen lateral de los huesos esfenóticos rectilíneo. Genidens sp. nov., representa el registro más antiguo para el género y constituiría una especie de aspecto similar a las formas actuales del género. sp. nov., representa el registro más antiguo para el género y constituiría una especie de aspecto similar a las formas actuales del género.

  15. Fontes não-convencionais de proteína na primeira alimentação do bagre sul americano (Rhamdia quelen Unconventional protein sources in the first feeding of South American catfish (Rhamdia quelen

    Directory of Open Access Journals (Sweden)

    David Roque Hernández

    2009-06-01

    Full Text Available O presente estudo teve como objetivo avaliar diferentes fontes de proteína na primeira alimentação de jundiá (Rhamdia quelen sob condições de laboratório. Foram testadas três rações balanceadas secas: ovo de peixe (TA, hidrolisado de carcaça do peixe (TB e fígado de frango cru (TC, na quais foram analisadas, pelo crescimento, a biomassa final produzida e a sobrevivência das larvas. O ensaio teve uma duração de 20 dias, e as rações foram fornecidas ad libitum. As larvas alimentadas com ovo de peixe apresentaram os melhores resultados com peso médio individual de 340mg, sobrevivência de 57% e biomassa final de 1.350mg, diferindo significativamente dos demais tratamentos. Os resultados indicaram que a formulação de rações balanceadas com ovos de peixes fornecida na primeira alimentação de larvas de jundiá pode tornar-se uma alternativa promissora na larvicultura dessa espécie.This study aimed to evaluate different proteins sources in first feeding of jundiá (Rhamdia quelen under laboratory conditions. Three balanced dry rations were tested, fish roe (TA, biological fish silage (TB and crude chicken liver (TC evaluating specific growth rate, final biomass produced and survival rate. The experiment was performed in 20 days, and all the rations were provided ad libitum. Larvae fed with fish roe presented better results, with 340mg of individual mean weight, 57% of survival rate and 1.350mg of final biomass, differing significantly with the other treatments. The results show that the inclusion of fish roe in balanced rations offered at the first feeding of jundiá larvae may become a promising alternative for the hatchering of the species.

  16. Acute Toxicity Of Euphorbia Royleana Boiss (Euphorbiaceae latex on freshwater catfish, Heteropneustes Fossilis (Siluriformes, Heteropneustidae Toxicidad aguda del látex de Euphorbia royleana Boiss (Euphorbiaceae en el bagre de agua dulce, Heteropneutes fossilis (Siluriformes, Heteropneustidae

    Directory of Open Access Journals (Sweden)

    Prasad ManiRam

    2010-06-01

    Full Text Available An acute toxicity test was performed by using a four-day static renewal test to determine the LC50 value of aqueous extract of Euphorbia royleana latex for the freshwater fsh, Heteropneustes fossilis. The LC50 values, their upper and lower confdence limits and slope functions were calculated. The LC50 values for aqueous extract of Euphorbia royleana latex at various exposure periods were 7.758 mg/L for 24 h, 5.847 mg/L for 48 h, 4.474 mg/L for 72 h and 3.090 mg/L for 96 h. The regression coeffcient showed that there was signifcant negative correlation between exposure time and different LC values. Hence, it is concluded that the concentration to produce toxicity of latex of Euphorbia royleana is comparable and close to the concentration to produce toxicity of synthetic organophosphates pesticides for the fsh H. fossilis. Therefore, adequate precautions must be taken when Euphorbia royleana latex is being used near fsh- inhabited areas.La prueba de la toxicidad aguda fue realizada utilizando un test estático con renovación, de cuatro días de duración, para determinar el valor de la CL50 de un extracto acuoso del látex de Euphorbia royleana, en el pez de agua dulce Heteropneustes fossilis. Se calcularon el valor de la CL50, los límites de confanza máximo y mínimo y la pendiente. Los valores de la CL50 para el extracto acuoso del látex en varios períodos de exposición fueron 7,758 mg/L para 24 h, 5,847 mg/L para 48 h, 4,474 mg/L para 72 h y 3,090 mg/L para 96 h. El coefciente de regresión mostró una correlación negativa signifcativa entre el tiempo de exposición y diferentes valores de la CL50. Se concluye que la concentración del látex de E. royleana que produce toxicidad es comparable y cercana a la de los plaguicidas sintéticos organofosforados para el pez H. fossilis. Por lo tanto, se deben tomar precauciones adecuadas cuando el látex de E. royleana es utilizado cerca de áreas donde habita el pez H. fossilis.

  17. Coinfection by Toxoplasma gondii and Leishmania spp. in domestic cats (Felis catus in State of Mato Grosso do Sul

    Directory of Open Access Journals (Sweden)

    Audrey Rennó Campos Braga

    2014-12-01

    Full Text Available Introduction Leishmaniasis and toxoplasmosis are important to public health. Methods Antibodies for Toxoplasma gondii and Leishmania spp. were evaluated in cats from Campo Grande, State of Mato Grosso do Sul, Brazil, a region endemic for canine visceral leishmaniasis. Serum samples from 50 asymptomatic cats were titrated for T. gondii by the immunofluorescence antibody test and modified agglutination test and for Leishmania spp. by the immunofluorescence antibody test. Results These two agents coinfected two (4% of the 50 tested animals. Conclusions These findings demonstrate the concomitant presence of two important zoonoses in cats from Brazilian endemic regions for canine visceral leishmaniasis.

  18. Expression profiles of relaxin family peptides and their receptors indicate their influence on spermatogenesis in the domestic cat (Felis catus).

    Science.gov (United States)

    Braun, B C; Müller, K; Jewgenow, K

    2015-07-01

    Disturbed spermatogenesis is a common problem in felines. Studying spermatogenesis in the domestic cat can improve the understanding of the biological background and help to counteract fertility problems in other feline species. Here, we analyzed 3 relaxin family peptides (relaxin, relaxin-3, and INSL3) and their receptors (RXFP1, RXFP2, and RXFP3) as potential spermatogenic factors involving their expression in the testis at different stages of its development. It may be concluded from its stage-dependent expression that relaxin, together with RXFP1, appears to be involved in the first stage of spermatogenesis, whereas relaxin-3 via binding to RXFP3 influences spermiogenesis. Furthermore, correlations were observed between relaxin, relaxin-3, RXFP1, RXFP2 and RXFP3 messenger RNA expression, and the relative numbers of haploid cells in testes. The peptide INSL3 was highly expressed at all testis development stages. Because of the low and stage-independent expression of its receptor RXFP2, an auto- and/or paracrine function of INSL3 in spermatogenesis seems unlikely. In the adult testis, messenger RNA expression of relaxin, RXFP1, and RXFP3 predominantly occurs in the tubular testis compartment, whereas INLS3 is mainly expressed in the interstitium. PMID:25704248

  19. Seroprevalence and Risk Factors Associated with Seropositivity to Toxoplasma gondii among Stray and Domestic Cats (Felis silvestris catus

    Directory of Open Access Journals (Sweden)

    Christel Bohn T. Garcia

    2014-12-01

    Full Text Available Toxoplasma gondii is a protozoan parasite that causes toxoplasmosis. It is widespread in the environment and infects a variety of warm-blooded animals, causing miscarriages and birth problems. Previous studies in the Philippines have determined the seropositivity of T. gondii in humans. However, the seroprevalence of the parasite among household pets, par ticularly its feline def initive host, remains insufficient . This study aimed to: (1 determine the seroprevalence of T. gondii antibodies among domestic and stray cats in the Philippines; and, (2 to analyze the risk factors associated with seropositivity. Blood samples from 59 domestic and stray cats were collected and tested for T. gondii seropositivity using a commercially available indirect ELISA kit, while pet owners and handlers were given questionnaires about their cats. Thirteen or 22.03% of the cats were seropositive to T. gondii, and risk factor analysis revealed a significant difference between domestic and stray cats with regard to diet (p = 0.026, OR = 8.333, c = 0.299 and domestication (p = 0.039, OR = 5.000, c = 0.276. Cats fed with table food tested 31.43% seropositive compared to the 4.35% of those fed with cat food, whereas 33.33% of the stray cats were seropositive compared to 7.69% for domestic cats. Odds ratio test showed that the risk factors studied were associated with higher likelihood of T. gondii seropositivity. These results implicate diet and environment in the transmission dynamics of T. gondii among cats.

  20. Haematology and biochemistry values of captive sand cats (Felis margarita in Al Ain Wildlife Park and Resort, United Arab Emirates

    Directory of Open Access Journals (Sweden)

    Stephen Chege

    2013-09-01

    Full Text Available Objective: To investigate the haematology and biochemistry values of apparently healthy captive sand cats kept in Al Ain Wildlife Park and Resort, United Arab Emirates, with a view to establishing baseline values. Methods: Blood was collected from the femoral vein using aseptic techniques, kept in a cool box and sent to laboratory for analysis. The blood was analysed for haematological and biochemical values using veterinary hematology and chemistry analysers (ABX ABC Vet, Horiba ABX SAS ® Montpellier, France. Results: Haematological values were within the normal ranges recorded in domestic cats and there was no statistical difference between values found in males and females. Aspartate aminotransferase values were higher (P0.05 between males and females values. Conclusions: Our results present reference ranges for haematology and biochemistry parameters in captive sand cats. These values will be important for diagnosis of various diseases and monitoring of treatments.

  1. L'areale italiano del gatto selvatico europeo (Felis silvestris silvestris: ancora un dilemma?

    Directory of Open Access Journals (Sweden)

    Bernardino Ragni

    2003-10-01

    Full Text Available Nel 1992 dall'Aspromonte l'area di distribuzione del gatto selvatico europeo si arrestava al parallelo UTM 4800000 Nord, su una linea corologica che andava da Piombino a Fabriano (Ragni et al. 1994. Nessuna spiegazione ecologica poteva giustificare i 450 chilometri di iato corologico che separava tale linea dal frammento di areale disgiunto delle Alpi Liguri; ad Est la stazione più prossima era ubicata nell'area del Cansiglio (Prealpi Venete. Dopo un decennio si presenta un aggiornamento sullo stato del "dilemma". Dal giugno 1992 al giugno 2003 sono stati raccolti 46 reperti (esemplari rinvenuti morti, catturati vivi o fotografati in natura in Toscana, Umbria e Marche, le regioni "tagliate" dal limite d'areale peninsulare. Uno solo dei reperti risulta rinvenuto a Nord di detto limite: "sui monti di Carpegna", in Provincia di Pesaro-Urbino all'altezza del parallelo UTM 4850000 Nord; si tratta di un maschio adulto, abbattuto illegalmente. La nuova stazione zoogeografica è separata dal limite del 1992 con un salto longitudinale di almeno 50 chilometri, così come i 12440 Kmq di Penisola Italiana compresi tra i due paralleli risultano completamente privi del felide. Analogamente a quello spaziale si osserva un salto temporale di 10 anni (giugno 1992-ottobre 2002 tra le due condizioni corologiche. Al contrario, i 13200 Kmq di penisola compresi tra il parallelo "storico" e quello posto 50 chilometri più a Sud, sono punteggiati da 35 reperti con distribuzione spaziale relativamente uniforme; così come la distribuzione delle stazioni nel tempo. Se si esclude il ritrovamento di Carpegna i limiti di areale definiti nel 1992 sono rimasti immutati: le nuove stazioni peninsulari confermano, al giugno 2003, la linea Piombino-Fabriano; le nuove stazioni Nord-orientali confermano, all'ottobre del 2002, l'area del Cansiglio; mentre, dopo il 1992, le stazioni della Liguria occidentale, che risalivano già al 1981, non hanno ricevuto alcuna conferma. In conclusione, dal punto di vista della dinamica d'areale F. s. silvestris in Italia si mostra, complessivamente, molto conservativo: un fenomeno noto anche per un altro felide, la lince eurasiatica nel suo areale alpino. La situazione sopra delineata, quindi, impone di considerare il ritrovamento di Carpegna con molta prudenza: per escludere la possibilità che si tratti delle conseguenze di un intervento antropico o di un evento naturale stocastico, occorre sviluppare un monitoraggio intensivo in adeguate aree di studio, al fine di verificare l'esistenza di una popolazione vitale del felide.

  2. EFFECTS OF SURGICAL STRESS ON THE SECRETION OF LUTEINIZING HORMONE, TESTOSTERONE AND CORTISOL IN THE DOMESTIC CAT (Felis catus

    Directory of Open Access Journals (Sweden)

    Gelson Genaro

    2006-10-01

    Full Text Available A influência do estresse sobre as diversas funções fisiológicas tem sido por longo tempo estudada. Observações de que situações de estresse são geralmente acompanhadas de alterações nas atividades reprodutivas são também conhecidas. Assim, utilizaram-se 21 gatos (machos,adultos em procedimento cirúrgico (24, 48 e 72 h, após canulação do vaso jugular direito, coletando-se sangue para dosagem, via radioimunoensaio, dos hormônios cortisol, testosterona e hormônio luteinizante (LH. Os resultados obtidos demonstraram que, para o gato doméstico, os níveis séricos dos hormônios estudados apresentaram uma elevação acentuada em suas concentrações, após o ato cirúrgico, e que diminuía gradativamente, voltando aos valores basais ao final de 48 h (2 ng/ml para o cortisol, 1ng/ml para testosteona, e 3,5 ng/ml para LH, aproximadamente, regularizando-se a seguir. Esses resultados demonstram que, após procedimento cirúrgico, os felinos têm suas concentrações especialmente de cortisol aumentadas significativamente. O intervalo de pelo menos três dias deve ser aguardado antes que se proceda a quaisquer outros processos de manipulação que envolvam o sistema endócrino dessa espécie animal, já que durante esse período as concentrações de cortisol, bem como de outros hormônios, estão alteradas. PALAVRAS-CHAVE: Cortisol, estresse, gato, LH, testosterona.

  3. Haematology and biochemistry values of captive sand cats (Felis margarita) in Al Ain Wildlife Park and Resort, United Arab Emirates

    Institute of Scientific and Technical Information of China (English)

    Stephen Chege; Arshad Toosy; Judith Howlett; Ahmed Saker; John Kagira

    2013-01-01

    Objective: To investigate the haematology and biochemistry values of apparently healthy captive sand cats kept in Al Ain Wildlife Park and Resort, United Arab Emirates, with a view to establishing baseline values.Methods:Blood was collected from the femoral vein using aseptic techniques, kept in a cool box and sent to laboratory for analysis. The blood was analysed for haematological and biochemical values using veterinary hematology and chemistry analysers (ABX ABC Vet, Horiba ABX SAS Montpellier, France). Results: Haematological values were within the normal ranges recorded in domestic cats and there was no statistical difference between values found in males and females. Aspartate aminotransferase values were higher (P0.05) between males and females values.Conclusions:Our results present reference ranges for haematology and biochemistry parameters in captive sand cats. These values will be important for diagnosis of various diseases and monitoring of treatments.

  4. Using population genetic tools to develop a control strategy for feral cats (Felis catus) in Hawai'i

    Science.gov (United States)

    Hansen, H.; Hess, S.C.; Cole, D.; Banko, P.C.

    2007-01-01

    Population genetics can provide information about the demographics and dynamics of invasive species that is beneficial for developing effective control strategies. We studied the population genetics of feral cats on Hawai'i Island by microsatellite analysis to evaluate genetic diversity and population structure, assess gene flow and connectivity among three populations, identify potential source populations, characterise population dynamics, and evaluate sex-biased dispersal. High genetic diversity, low structure, and high number of migrants per generation supported high gene flow that was not limited spatially. Migration rates revealed that most migration occurred out of West Mauna Kea. Effective population size estimates indicated increasing cat populations despite control efforts. Despite high gene flow, relatedness estimates declined significantly with increased geographic distance and Bayesian assignment tests revealed the presence of three population clusters. Genetic structure and relatedness estimates indicated male-biased dispersal, primarily from Mauna Kea, suggesting that this population should be targeted for control. However, recolonisation seems likely, given the great dispersal ability that may not be inhibited by barriers such as lava flows. Genetic monitoring will be necessary to assess the effectiveness of future control efforts. Management of other invasive species may benefit by employing these population genetic tools. ?? CSIRO 2007.

  5. Anticipation is differently expressed in rats (Rattus norvegicus) and domestic cats (Felis silvestris catus) in the same Pavlovian conditioning paradigm

    NARCIS (Netherlands)

    Bos, van den R.; Meijer, M.K.; Renselaar, van J.; Harst, van der J.E.; Spruijt, B.M.

    2003-01-01

    In rats (Rattus norvegicus) anticipation to an oncoming food reward in an appetitive Pavlovian conditioning procedure is expressed as an increase of behavioural transitions, i.e. hyperactivity. This behaviour might be related to the spontaneous appetitive behaviour of animals in relation to oncoming

  6. Detection of Babesia hongkongensis sp. nov. in a Free-Roaming Felis catus Cat in Hong Kong

    OpenAIRE

    Wong, Samson S. Y.; Poon, Rosana W. S.; Hui, Janet J. Y.; Yuen, Kwok-Yung

    2012-01-01

    Intraerythrocytic Babesia-like trophozoites were seen in postmortem kidney sections of a free-roaming cat in Hong Kong. DNA sequences of the 18S rRNA and mitochondrial cytochrome b genes had only 96.7% and 90.4% nucleotide identity with known Babesia sequences. We propose that this new species be named Babesia hongkongensis.

  7. Comparative speed of kill of sarolaner (Simparica™) and fluralaner (Bravecto®) against induced infestations of Ctenocephalides felis on dogs

    OpenAIRE

    Six, Robert H.; Liebenberg, Julian; Honsberger, Nicole A.; Mahabir, Sean P.

    2016-01-01

    Background Fleas are the most common ectoparasite infesting dogs globally and cause direct discomfort, induce allergic reactions, and transmit pathogenic agents. Rapid speed of kill is an important characteristic for a parasiticide in order to alleviate the direct deleterious effects of fleas, reduce the impact of allergic responses, and break the flea life cycle. In this study, the speed of kill of a novel, orally administered isoxazoline parasiticide, sarolaner (Simparica™), against fleas o...

  8. Are cats (Felis catus) from multi-cat households more stressed? Evidence from assessment of fecal glucocorticoid metabolite analysis.

    Science.gov (United States)

    Ramos, D; Reche-Junior, A; Fragoso, P L; Palme, R; Yanasse, N K; Gouvêa, V R; Beck, A; Mills, D S

    2013-10-01

    Given the social and territorial features described in feral cats, it is commonly assumed that life in multi-cat households is stressful for domestic cats and suggested that cats kept as single pets are likely to have better welfare. On the other hand, it has been hypothesized that under high densities cats can organize themselves socially thus preventing stress when spatial dispersion is unavailable. This study was aimed at comparing the general arousal underpinning emotional distress in single housed cats and in cats from multi-cat households (2 and 3-4 cats) on the basis of fecal glucocorticoid metabolites (GCM) measured via enzyme immunoassay (EIA). GCM did not significantly vary as a function of living style (single, double or group-housing); highly stressed individuals were equally likely in the three groups. Young cats in multi-cat households had lower GCM, and overall cats that tolerate (as opposed to dislike) petting by the owners tended to have higher GCM levels. Other environmental aspects within cat houses (e.g. relationship with humans, resource availability) may play a more important role in day to day feline arousal levels than the number of cats per se. PMID:24021924

  9. INDUCTION OF NITRIC OXIDE SYNTHASE AND ASSOCIATED TOXICITY IN LIVERS OF HARDHEAD CATFISH, ARIUS FELIS, FROM CONTROL AND EPIZOOTIC SITES

    Science.gov (United States)

    Earlier work with a live channel catfish (Ictalurus punctatus) pathogen, Edwardsiella ictaluri, demonstrated the induction of nitric oxide synthase (NOS) in the head kidney, paralleling enteric septicemia (Hawke et al. 1981; Schoor and Plumb 1994). However, another study exposing...

  10. Propionate absorbed from the colon acts as gluconeogenic substrate in a strict carnivore, the domestic cat (Felis catus)

    DEFF Research Database (Denmark)

    Verbrugghe, A; Hesta, M; Daminet, S;

    2012-01-01

    In six normal-weight and six obese cats, the metabolic effect of propionate absorbed from the colon was assessed. Two colonic infusions were tested in a crossover design with intervals of 4 weeks. The test solution contained 4 mmol sodium propionate per kg ideal body weight in a 0.2% NaCl solution...

  11. Taxonomy Icon Data: domestic cat [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available tris_catus_NS.png http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Felis+silvestris+catus&t=L http://biosciencedbc.jp/taxonomy..._icon/icon.cgi?i=Felis+silvestris+catus&t=NL http://biosciencedbc.jp/taxonomy..._icon/icon.cgi?i=Felis+silvestris+catus&t=S http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Felis+silvestris+catus&t=NS ...

  12. Mercury concentrations in gafftopsail catfish and other fishes in waters adjacent to Hobe Sound National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From September 22-26, 1990, 20 gafftopsail catfish (Bagre marinus) and 3 other fish species (n=4) were collected from marine waters adjacent to Hobe Sound National...

  13. Mercury in fishes of the J.N. "Ding" Darling National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From June 26 to 30,1990 spotted gar (Lepisosteus oculatus), spotted seatrout (Cynoscion nebulosus), gafftopsail catfish (Bagre marinus), and largemouth bass...

  14. Isolation and characterization of viable Toxoplasma gondii isolates revealed possible high frequency of mixed infection in feral cats (felis domesticus) from St. Kitts, West Indies

    Science.gov (United States)

    Cats are essential in the epidemiology of Toxoplasma gondii because they are the only hosts that can excrete the environmentally resistant oocysts in nature. Samples of serum, feces, and tissues from feral cats from St. Kitts, West Indies were examined for T. gondii infection. Antibodies to T. gon...

  15. Rickettsia typhi IN RODENTS AND R. felis IN FLEAS IN YUCATÁN AS A POSSIBLE CAUSAL AGENT OF UNDEFINED FEBRILE CASES

    OpenAIRE

    PENICHE-LARA, Gaspar; Dzul-Rosado, Karla; PÉREZ-OSORIO, Carlos; Zavala-Castro, Jorge

    2015-01-01

    Rickettsia typhi is the causal agent of murine typhus; a worldwide zoonotic and vector-borne infectious disease, commonly associated with the presence of domestic and wild rodents. Human cases of murine typhus in the state of Yucatán are frequent. However, there is no evidence of the presence of Rickettsia typhi in mammals or vectors in Yucatán. The presence of Rickettsia in rodents and their ectoparasites was evaluated in a small municipality of Yucatán using the conventional polymerase chai...

  16. Region-specific localization of NOS isoforms and NADPH-diaphorase activity in the intratesticular and excurrent duct systems of adult domestic cats (Felis catus).

    Science.gov (United States)

    Liman, Narin; Alan, Emel

    2016-03-01

    Nitric oxide (NO) is produced by nitric oxide synthases (NOSs) and plays an important role in all levels of reproduction from the brain to the reproductive organs. Recently, it has been discovered that all germ cells and Leydig cells in the cat testis exhibit stage-dependent nuclear and cytoplasmic endothelial (eNOS) and inducible (iNOS)-NOS immunoreactivity and cytoplasmic nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) reactivity. As a continuation of this finding, in this study, cellular localization of NADPH-d and immunolocalization and expression of all three NOS isoforms were investigated in the intratesticular (tubuli recti and rete testis), and excurrent ducts (efferent ductules, epididymal duct and vas deferens) of adult cats using histochemistry, immunohistochemistry and western blotting. NADPH-d activity was found in the midpiece of the spermatozoa tail and epithelial cells of all of ducts, except for nonciliated cells of the efferent ductules. Even though the immunoblotting results revealed similar levels of nNOS, eNOS and iNOS in the caput, corpus and cauda segments of epididymis and the vas deferens, immunostainings showed cell-specific localization in the efferent ductules and region- and cell-specific localization in the epididymal duct. All of three NOS isoforms were immunolocalized to the nuclear membrane and cytoplasm of the epithelial cells in all ducts, but were found in the tail and the cytoplasmic droplets of spermatozoa. These data suggest that NO/NOS activity might be of importance not only for the functions of the intratesticular and excurrent ducts but also for sperm maturation. PMID:26910642

  17. Ecología del gato montés ("Felis silvestris") y su relación con el conejo de monte ("Oryctolagus cuniculus")

    OpenAIRE

    Lozano Mendoza, Jorge

    2008-01-01

    Se ha estudiado la ecología del gato montés y su relación particular con el conejo de monte tanto en preferencias de hábitat, dieta, relaciones con ungulados y tendencias de población. El gato montés fue más abundante a escala de paisaje en los mosaicos formados por matorral y pastizal, siendo el matorral además la variable de hábitat más claramente asociada con la especie en todas las escalas espaciales. Por tanto, se identifica al matorral como un elemento estructural del medio clave para l...

  18. Short term preservation of chilled tomcat (Felis catus L.) spermatozoa, obtained by urethral catheterization after medetomidine administration, diluted with a laboratory prepared extender

    OpenAIRE

    Constantin Pavli; Irina O. Tănase

    2013-01-01

    Semen collection in tomcat represents the first step to establish a breeding protocol. Therefore we used a method of harvesting that involves little difficulties and discomfort for the test male. The method was described previously by Zambelli (2006) and is represented by semen collection by a tomcat catheter, after medetomidine administration. In a few cases, when was possible, semen was also collected by electroejaculation, the males were housed in individual cages and collectio...

  19. AVALIAÇÃO DE SEGURANÇA E ANALGESIA DE PROTOCOLOS ANESTÉSICOS PARA ELETROEJACULAÇÃO EM GATOS DOMÉSTICOS (Felis catus

    Directory of Open Access Journals (Sweden)

    TICIANA FRANCO PEREIRA DA SILVA

    2011-09-01

    Full Text Available The objective of this study was to evaluate the security and analgesia of anesthetics protocols usually used for electroejaculation (EEJ in domestic cats. Fourteen toms were anesthetized with 4 protocols and submitted to a 3 series of electric stimuli (2-6 mA. The heart rate, respiratory rate, temperature, pain sensibility and eyes blink were analyzed prior and after induction, and during and after electroejaculation. The anesthesia protocol thatused isoflurane was the best for analgesic parameters, security and speed of recuperation for electroejaculation in domestic cats

  20. AVALIAÇÃO DE SEGURANÇA E ANALGESIA DE PROTOCOLOS ANESTÉSICOS PARA ELETROEJACULAÇÃO EM GATOS DOMÉSTICOS (Felis catus)

    OpenAIRE

    TICIANA FRANCO PEREIRA DA SILVA; CARLOS GABRIEL ALMEIDA DIAS; CAMILA LOUISE ACKERMANN; FRANCISCO TIAGO SILVA PINHEIRO; ANA CRISTINA PAULINO BRAGA; LÚCIA DANIEL MACHADO DA SILVA

    2011-01-01

    The objective of this study was to evaluate the security and analgesia of anesthetics protocols usually used for electroejaculation (EEJ) in domestic cats. Fourteen toms were anesthetized with 4 protocols and submitted to a 3 series of electric stimuli (2-6 mA). The heart rate, respiratory rate, temperature, pain sensibility and eyes blink were analyzed prior and after induction, and during and after electroejaculation. The anesthesia protocol thatused isoflurane was the best for analgesic pa...

  1. Isoflavone metabolism in domestic fcats (Felis catus): comparison of plasma metabolites detected after ingestion of two different dietary forms of genistein daidzein

    NARCIS (Netherlands)

    Whitehouse-Tedd, K.; Cave, N.J.; Ugarte, C.E.; Waldron, L.A.; Prasain, J.K.; Arabshahi, A.; Barnes, S.; Hendriks, W.H.; Thomas, D.G.

    2013-01-01

    Some felid diets contain isoflavones but the metabolic capacity of cats towards isoflavones is relatively unknown, despite the understanding that isoflavones have divergent biological potential according to their metabolite end-products. The objective of this study was to determine the plasma metabo

  2. Antibody detection and molecular characterization of toxoplasma gondii from bobcats (Lynx rufus), domestic cats (Felis catus), and wildlife from Minnesota, USA

    Science.gov (United States)

    Little is known of the epidemiology of toxoplasmosis in Minnesota. In this study, we evaluated Toxoplasma gondii infection in 50 wild bobcats (Lynx rufus) and 75 other animals on/near 10 cattle farms. Antibodies to T. gondii were assayed in serum samples or tissue fluids by the modified agglutinatio...

  3. Toward a genome-wide approach for detecting hybrids: informative SNPs to detect introgression between domestic cats and European wildcats (Felis silvestris).

    Science.gov (United States)

    Oliveira, R; Randi, E; Mattucci, F; Kurushima, J D; Lyons, L A; Alves, P C

    2015-09-01

    Endemic gene pools have been severely endangered by human-mediated hybridization, which is posing new challenges in the conservation of several vertebrate species. The endangered European wildcat is an example of this problem, as several natural populations are suffering introgression of genes from the domestic cat. The implementation of molecular methods for detecting hybridization is crucial for supporting appropriate conservation programs on the wildcat. In this study, genetic variation at 158 single-nucleotide polymorphisms (SNPs) was analyzed in 139 domestic cats, 130 putative European wildcats and 5 captive-bred hybrids (N=274). These SNPs were variable both in wild (HE=0.107) and domestic cats (HE=0.340). Although we did not find any SNP that was private in any population, 22 SNPs were monomorphic in wildcats and pairwise FCT values revealed marked differences between domestic and wildcats, with the most divergent 35 loci providing an average FCT>0.74. The power of all the loci to accurately identify admixture events and discriminate the different hybrid categories was evaluated. Results from simulated and real genotypes show that the 158 SNPs provide successful estimates of admixture, with 100% hybrid individuals (two to three generations in the past) being correctly identified in STRUCTURE and over 92% using the NEWHYBRIDS' algorithm. None of the unclassified cats were wrongly allocated to another hybrid class. Thirty-five SNPs, showing the highest FCT values, provided the most parsimonious panel for robust inferences of parental and first generations of admixed ancestries. This approach may be used to further reconstruct the evolution of wildcat populations and, hopefully, to develop sound conservation guidelines for its legal protection in Europe. PMID:26103945

  4. Absence of zoonotic Bartonella species in questing ticks: First detection of Bartonella clarridgeiae and Rickettsia felis in cat fleas in the Netherlands

    NARCIS (Netherlands)

    Tijsse-Klasen, E.; Fonville, M.; Gassner, F.; Nijhof, A.M.; Hovius, E.K.E.; Jongejan, F.; Takken, W.; Reimerink, J.R.; Overgaauw, P.A.M.; Sprong, H.

    2011-01-01

    Background: Awareness for flea-and tick-borne infections has grown in recent years and the range of microorganisms associated with these ectoparasites is rising. Bartonella henselae, the causative agent of Cat Scratch Disease, and other Bartonella species have been reported in fleas and ticks. The r

  5. SEROPREVALENCE OF Toxoplasma gondii (Nicole & Manceaux, 1909 AND RETROVIRAL STATUS OF CLIENT-OWNED PET CATS (Felis catus, Linnaeus, 1758 IN RIO DE JANEIRO, BRAZIL

    Directory of Open Access Journals (Sweden)

    Bethânia Ferreira Bastos

    2014-06-01

    Full Text Available Cats, as definitive host, play an important role in the transmission of Toxoplasma gondii. This study aimed to establish the seroprevalence of anti-T. gondii immunoglobulins G and M, and determine the frequency of oocysts in the feces of the domestic cat population in Rio de Janeiro, Brazil. We also aimed to study the association between T. gondii infection and age, sex, breed, lifestyle, diet and retroviral infection. A total of 108 cats were included in the study and fecal samples of 54 of those cats were obtained. Only 5.6% of the cats were seropositive for anti-T. gondii immunoglobulins using the indirect hemagglutination test. None of the 54 cats presented oocysts in their fecal samples. Although not statistically significant, males, mixed-breed, free-roaming and cats aged two years and older were found to be more exposed. Age, lifestyle and the use of litter boxes were found to play an important role as risk factors. Anemia and retroviral infections were independent of T. gondii infection. No antibodies were detected in the majority of cats (94.4%, indicating that those cats had never been exposed to the parasite and, therefore, once infected, they could present the risk of shedding large numbers of oocysts into the environment.

  6. Nuclear and microtubule remodeling and in vitro development of nuclear transferred cat oocytes with skin fibroblasts of the domestic cat (Felis silvestris catus) and leopard cat (Prionailurus bengalensis).

    Science.gov (United States)

    Yin, X J; Lee, Y H; Jin, J Y; Kim, N H; Kong, I K

    2006-10-01

    The leopard cat (Prionailurus bengalensis), a member of the felidae family, is a threatened animal in South Korea. In terms of protecting endangered felids, nuclear transfer (NT) is a potentially valuable technique for assuring the continuation of species with dwindling numbers. In the present experiment, nuclear and microtubule remodeling and the in vitro developmental potential of enucleated domestic cat oocytes reconstructed with nuclei of somatic cells from either domestic cat fibroblast (DCF) or leopard cat fibroblast (LCF) were evaluated. Microtubule aster is allocated to de-condensed chromatin following nuclear transfer (3h after activation) of fibroblast cells from both domestic and leopard cats, suggesting the introduction of a somatic cell centrosome. The transferred fibroblast nuclei formed a large, swollen, pronuclear-like structure in most reconstructed oocytes, in the cat or leopard cat. At 18h following nuclear transfer, mitosis occurred, and according to the photo (F) it appears that spindle microtubules and two asters were observed. The percentages of blastocyst formation from nuclear transfer embryos derived from domestic cat fibroblasts (4/46, 8.6%) were not significantly different than those for nuclear transfer embryos constructed with leopard cat fibroblasts (4/52, 7.6%). These results indicate that nuclear and microtubule remodeling processes and in vitro developmental ability are similar in reconstructed cat oocytes following transfer of nuclei from either domestic or leopard cats. PMID:16310987

  7. Uso de la ecografía para el bloqueo de los nervios periféricos del miembro pelviano en el gato (Felis catus L.)

    OpenAIRE

    Haro Álvarez, Ana Paulina

    2013-01-01

    El presente estudio fue llevado a cabo para describir la apariencia ecográfica y desarrollar los abordajes ecoguiados para el bloqueo de los nervios ciático y femoral, así como evaluar la eficacia clínica del bloqueo ecoguiado del nervio ciático en el gato. Se realizaron disecciones anatómicas y criosecciones para determinar las marcas anatómicas para localizar los nervios de interés. La apariencia ecográfica de los nervios ciático y femoral fue estudiada tanto en cadáveres como en gatos vivo...

  8. Comparative speed of kill of sarolaner (Simparica™) and afoxolaner (NexGard®) against induced infestations of Ctenocephalides felis on dogs

    OpenAIRE

    Six, Robert H.; Liebenberg, Julian; Honsberger, Nicole A.; Mahabir, Sean P.

    2016-01-01

    Background Fleas are the most common ectoparasite infesting dogs globally. The many possible sequellae of infestation include: direct discomfort; allergic reactions; and the transmission of pathogens. Rapid speed of kill is an important characteristic for a parasiticide in order to alleviate the direct deleterious effects of fleas, reduce the impact of allergic responses, and break the flea infestation cycle. In this study, the speed of kill of a novel orally administered isoxazoline parasiti...

  9. Comparative speed of kill of sarolaner (Simparica™) and spinosad plus milbemycin oxime (Trifexis®) against induced infestations of Ctenocephalides felis on dogs

    OpenAIRE

    Six, Robert H.; Everett, William R.; Myers, Melanie R.; Mahabir, Sean P.

    2016-01-01

    Background Fleas are a ubiquitous ectoparasite infesting dogs and cause direct discomfort, allergic reactions and are responsible for the transmission of several pathogens. The rapid speed of kill of a parasiticide is important to alleviate the direct deleterious effects of fleas, reduce the impact of allergic responses, and break the flea life cycle. In this study, the speed of kill of a novel orally administered isoxazoline parasiticide, sarolaner (Simparica™) against fleas on dogs was eval...

  10. Anatomical study of the forearm and hand nerves of the domestic cat ( Felis catus), puma ( Puma concolor) and jaguar ( Panthera onca).

    Science.gov (United States)

    Sánchez, H L; Silva, L B; Rafasquino, M E; Mateo, A G; Zuccolilli, G O; Portiansky, E L; Alonso, C R

    2013-04-01

    The innervation of the forearm and hand regions of cats has not been well described despite its importance for any surgery or any neurological disorder. It is probably the main area where disorders of peripheral nerves in this species are observed. In felines, the forelimbs facilitate the jump and represent the most important way for capturing prey. The main muscles and nerves involved in this activity are located in the region of the forearm and hand. The aim of the present study was to provide a detailed description of the innervation of the forearm and hand regions of the jaguar and puma, in comparison with that of the domestic cat, contributing thus with the anatomical knowledge of the area for applying it to surgery and pathology. The forearms of three pumas and two jaguars (all of them fixed in formalin) and of six domestic cats (fresh) were dissected. The nerves path and their forearm distribution patterns of all three species were described. The analysed results indicate that the observed variations between species are minimal; thus, the anatomy described for domestic cats can be widely applied to American wild felids.

  11. Exame parasitológico de fezes de gatos (Felis catus domesticus domiciliados e errantes da Região Metropolitana do Rio de Janeiro, Brasil

    Directory of Open Access Journals (Sweden)

    Serra Cathia Maria Barrientos

    2003-01-01

    Full Text Available Foram estudadas 131 amostras de fezes de gatos de comportamento domiciliado e errante da Região Metropolitana do Rio de Janeiro, obtendo-se uma positividade de 63,4% das amostras, com maior ocorrência no grupo de animais errantes. Foi observado predomínio de parasitismo por Ancylostoma sp (43,5%, Toxocara sp (19,1% e Cystoisospora sp (43,5% em ambos os grupos. Também foram encontrados ovos de Uncinaria sp (1,5%, Toxascaris leonina (7,6%, cistos de Giardia sp (6,1% e esporocistos de Sarcocystis sp (0,8%. A alta prevalência de enteroparasitas na amostra estudada ressalta a importância de um maior controle parasitológico nesses animais, para proteção da saúde animal e humana.

  12. Uso de la ecografía para el bloqueo de nervios periféricos del miembro torácico en el gato (Felis catus L:)

    OpenAIRE

    Ansón Fernández, Agustina

    2016-01-01

    Objetivos 1. Describir los abordajes ecográficos para la evaluación del plexo braquial (PB) y los principales nervios del miembro torácico, así como la anatomía y apariencia ecográfica normales correlacionando las imágenes ecográficas con la disección anatómica y las criosecciones. 2. Establecer los abordajes ecográficos para el bloqueo ecoguiado del PB. 3. Determinar la eficacia de los diferentes abordajes para el bloqueo anestésico ecoguiado del PB, mediante la evaluación de la d...

  13. The 18-kDa form of cat allergen Felis domesticus 1 (Fel d 1) is associated with gelatin- and fibronectin-degrading activity

    DEFF Research Database (Denmark)

    Ring, P C; Wan, H; Schou, C;

    2000-01-01

    BACKGROUND: Fel d 1, an important allergen from domestic cats, is a significant cause of asthma. In addition to directly promoting IgE synthesis, other biological activities of allergens may contribute to either allergic sensitization or the magnitude of allergic effector responses. For example...

  14. Espectro trófico del bagre Ictalurus punctatus (Silurifomes: Ictaluridae), en la presa Lázaro Cárdenas, Indé, Durango, México Trophic spectrum of the catfish Ictalurus punctatus (Siluriformes: Ictaluridae), in the Lázaro Cárdenas reservoir, Indé, Durango, Mexico

    OpenAIRE

    Gabriel Fernando Cardoza Martínez; José Luis Estrada Rodríguez; Fernando Alonzo Rojo; Carmen Leticia Mar Tovar; Frances Gelwick

    2011-01-01

    Se analizó el contenido de 240 estómagos de Ictalurus punctatus (Rafinesque, 1818), colectados de 2006 a 2007, con el objetivo de determinar su espectro trófico, su variación por talla y época climática. Los contenidos estomacales se analizaron en laboratorio y los organismos se determinaron hasta nivel de orden. Los datos obtenidos fueron analizados por métodos cuantitativos y cualitativos. Los órdenes más frecuentes fueron Perciformes y Atheriniformes que corresponden a peces forraje, así c...

  15. Espectro trófico del bagre Ictalurus punctatus (Silurifomes: Ictaluridae, en la presa Lázaro Cárdenas, Indé, Durango, México Trophic spectrum of the catfish Ictalurus punctatus (Siluriformes: Ictaluridae, in the Lázaro Cárdenas reservoir, Indé, Durango, Mexico

    Directory of Open Access Journals (Sweden)

    Gabriel Fernando Cardoza Martínez

    2011-08-01

    Full Text Available Se analizó el contenido de 240 estómagos de Ictalurus punctatus (Rafinesque, 1818, colectados de 2006 a 2007, con el objetivo de determinar su espectro trófico, su variación por talla y época climática. Los contenidos estomacales se analizaron en laboratorio y los organismos se determinaron hasta nivel de orden. Los datos obtenidos fueron analizados por métodos cuantitativos y cualitativos. Los órdenes más frecuentes fueron Perciformes y Atheriniformes que corresponden a peces forraje, así como algas verdes del orden Charales. Además, se encontraron otros siete órdenes de invertebrados: Schizodonta, Odonata, Himenoptera, Orthoptera, Hemiptera, Homoptera y Scorpionida, además de dos órdenes de plantas: Poales y Fabales. El orden Perciforme constituyó casi la mitad del total del peso del contenido estomacal, seguido por los órdenes Atheriniforme y Charales. En las estaciones de verano e invierno se presentó el mayor número de estómagos vacíos, mientras que en primavera se encontró el mayor número de estómagos llenos. La cantidad de alimento ingerida en la estación de invierno fue significativamente menor que en las demás estaciones. El principal alimento en primavera, verano y otoño, fueron los peces, mientras que en invierno hubo mayor preferencia por las algas.Having the objective of determining their trophic spectrum and their variations within a year, the contents of 240 stomachs of Ictalurus punctatus (Rafinesque, 1818 were analyzed from 2006 to 2007. The items of the stomach contents were determined to the order level. The data was analyzed by different quantitative and qualitative methods. The most frequent orders were Perciforms and Atheriniforms corresponding to forage fish, along with green algae of the Charales order. Also, seven orders of invertebrates were found: Schizodonta, Odonata, Himenoptera, Orthoptera, Hemiptera, Homoptera and Scorpionida, and two orders of plants: Poales and Fabales. The Perciform order made up almost half of the total weight of the stomach contents, followed by the Atheriniform and the Charales orders. The greatest number of empty stomachs showed up during summer and winter, while the greatest number of filled-up stomachs was found during the spring season. The quantity of food consumed during winter time was significantly lower than the amount consumed during all other seasons. The kind of food consumed during the spring, summer and autumn was mainly fish, while algae were preferred during the winter season.

  16. NCBI nr-aa BLAST: CBRC-MLUC-01-0975 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MLUC-01-0975 ref|NP_001009324.1| melanocortin 1 receptor [Felis catus] dbj|BAC...76764.1| melanocortin 1 receptor [Felis catus] emb|CAQ86663.2| melanocortin 1 receptor [Felis catus] NP_001009324.1 1e-150 82% ...

  17. NCBI nr-aa BLAST: CBRC-OPRI-01-0259 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OPRI-01-0259 ref|NP_001129442.1| chemokine (C-C motif) receptor 3 [Felis catus...] gb|ACI42420.1| chemokine (C-C motif) receptor 3 [Felis catus] gb|ACJ13514.1| CC chemokine receptor 3 [Felis catus] NP_001129442.1 1e-140 71% ...

  18. NCBI nr-aa BLAST: CBRC-MLUC-01-0017 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MLUC-01-0017 ref|NP_001129441.1| chemokine (C-C motif) receptor 1 [Felis catus...] gb|ACI42419.1| chemokine (C-C motif) receptor 1 [Felis catus] gb|ACJ13513.1| CC chemokine receptor 1 [Felis catus] NP_001129441.1 1e-118 78% ...

  19. NCBI nr-aa BLAST: CBRC-VPAC-01-0317 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-VPAC-01-0317 ref|NP_001129441.1| chemokine (C-C motif) receptor 1 [Felis catus...] gb|ACI42419.1| chemokine (C-C motif) receptor 1 [Felis catus] gb|ACJ13513.1| CC chemokine receptor 1 [Felis catus] NP_001129441.1 1e-148 78% ...

  20. NCBI nr-aa BLAST: CBRC-VPAC-01-1032 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-VPAC-01-1032 ref|NP_001129442.1| chemokine (C-C motif) receptor 3 [Felis catus...] gb|ACI42420.1| chemokine (C-C motif) receptor 3 [Felis catus] gb|ACJ13514.1| CC chemokine receptor 3 [Felis catus] NP_001129442.1 5e-32 52% ...

  1. NCBI nr-aa BLAST: CBRC-FCAT-01-0370 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FCAT-01-0370 gb|ABP48095.1| CC chemokine receptor type 5 [Felis catus] gb|ABP4...8096.1| CC chemokine receptor type 5 [Felis catus] gb|ABP48097.1| CC chemokine receptor type 5 [Felis catus] ABP48095.1 0.0 99% ...

  2. NCBI nr-aa BLAST: CBRC-MEUG-01-2041 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MEUG-01-2041 ref|NP_001129442.1| chemokine (C-C motif) receptor 3 [Felis catus...] gb|ACI42420.1| chemokine (C-C motif) receptor 3 [Felis catus] gb|ACJ13514.1| CC chemokine receptor 3 [Felis catus] NP_001129442.1 1e-144 70% ...

  3. COMPARISON BETWEEN TWO METHODS OF STAINING FOR ASSESSMENT OF MORPHOLOGY AND ACROSOME IN DOMESTIC CAT (Felis catus SPERMATOZOA COMPARAÇÃO ENTRE DOIS MÉTODOS DE COLORAÇÃO PARA ANÁLISE MORFOLÓGICA E ACROSSOMAL DE ESPERMATOZÓIDES DE GATO DOMÉSTICO (Felis catus.

    Directory of Open Access Journals (Sweden)

    Frederico Ozanam Papa

    2008-10-01

    Full Text Available

    The objective of this study was to evaluate the efficiency of the modified Karras staining technique (KA to analyze domestic cat sperm morphology by comparing it with the Fast Green FCF/ Rose Bengal staining (FR, previously used for this species. Four adult cats were used, from which sperm samples were collected four times in alternate days for each tom using an artificial vagina (n=16 ejaculates. Both staining techniques were performed for each ejaculate. For the FR staining technique, the semen in natura was diluted in 2.9% sodium citrate and, afterwards, in the staining solution. After 70 seconds, smears were made onto slide and dried at 37ºC. For the KA staining technique, previously made and formol saline fixed slides were sequentially immersed in Rose Bengal solution, Tannin solution, and Victoria Blue B solution, and dried at room temperature. For sperm evaluation, 200 sperm cells were assessed for each staining technique in all ejaculate samples using a bright field microscope at 1000X magnification. Statistical analysis used the non-parametric Wilcoxon test, establishing significance at p<0.05. For the KA staining technique, higher percentage of distal cytoplasmic droplets and lower percentage of sperm head defects were obtained when compared to the FR staining technique. This way, both staining techniques were not totally efficient for the assessment of morphological defects found in the domestic cat in natura spermatozoa.

    KEY WORDS: Acrosome, domestic cat, spermatozoa, sperm morphology, staining.

     

    O objetivo do estudo foi avaliar a eficiência do método de coloração Karras modificado (KA para a análise da morfologia espermática no gato doméstico através da comparação com a coloração Fast Green FCF/Rosa Bengala (FR, previamente utilizada para esta espécie. Utilizaram-se quatro gatos adultos, colhendo-se quatro vezes amostras de sêmen em dias alternados para cada animal através de vagina artificial (n=16 ejaculados. Para cada ejaculado, realizaram-se duas colorações. Para a coloração FR, o sêmen in natura foi diluído em citrato de sódio 2,9% e, posteriormente, em solução de coloração. Após setenta segundos, procedeu-se a esfregaços em lâminas, as quais foram secas a 37ºC. Para a coloração KA, os esfregaços previamente confeccionados e fixados em formol salino foram imersos seqüencialmente nas soluções de Rosa Bengala, Tanino e Azul Vitória e secas em temperatura ambiente. Avaliaram-se duzentas células para cada tipo de coloração em todos os ejaculados, usando-se microscópio de luz em aumento de 1.000X. Efetuou-se análise estatística mediante o teste não-paramétrico de Wilcoxon, estabelecendo diferença significativa quando p<0,05. Para a coloração de KA, obtiveram-se maior porcentagem de gota citoplasmática distal e menor porcentagem de defeitos de cabeça quando comparada à coloração FR. Assim, nenhuma das colorações mostrou-se totalmente eficiente na identificação dos defeitos de morfologia encontrados na avaliação do sêmen in natura de gatos domésticos.

    PALAVRAS-CHAVES: Acrossomo, coloração, espermatozóide, gato doméstico, morfologia espermática.

     

  4. Occurrence of Leishmania (Leishmania chagasi in a domestic cat (Felis catus in Andradina, São Paulo, Brazil: case report Ocorrência de Leishmania (Leishmania chagasi em gato doméstico (Felis catus em Andradina, São Paulo, Brasil: relato de caso

    Directory of Open Access Journals (Sweden)

    Willian Marinho Dourado Coelho

    2010-12-01

    Full Text Available This work describes natural infection by Leishmania in a domestic cat where amastigote forms of the parasite were observed in the popliteal lymph node imprint. Positive and negative serological reactions were observed by enzyme-linked immunosorbent assay (ELISA and indirect immunofluorescence assay (IFA, respectively. Polymerase chain reaction (PCR revealed that the nucleotide sequence of the sample was identical to Leishmania (L. chagasi. This is the first report of the disease in felines of the city of Andradina, SP, an area considered endemic for canine and human visceral leishmaniasis.Neste trabalho, é relatada a infecção natural por Leishmania em um gato doméstico no qual, formas amastigotas do parasito foram observadas em imprint de linfonodo poplíteo. Reações sorológicas positivas e negativas foram observadas pelo teste de imunoadsorção enzimática (ELISA e reação de imunofluorescência indireta (RIFI, respectivamente. A reação em cadeia da polimerase (PCR revelou que a sequência de nucleotídeos foi idêntica à Leishmania (L. chagasi. Este é o primeiro relato da doença em felino da cidade de Andradina, Estado de São Paulo, Brasil, área considerada endêmica para leishmaniose visceral canina e humana.

  5. Occurrence of Leishmania (Leishmania) chagasi in a domestic cat (Felis catus) in Andradina, São Paulo, Brazil: case report Ocorrência de Leishmania (Leishmania) chagasi em gato doméstico (Felis catus) em Andradina, São Paulo, Brasil: relato de caso

    OpenAIRE

    Willian Marinho Dourado Coelho; Valéria Marçal Felix de Lima; Alessandro Francisco Talamini do Amarante; Helio Langoni; Virgínia Bodelão Richini Pereira; Aziz Abdelnour; Katia Denise Saraiva Bresciani

    2010-01-01

    This work describes natural infection by Leishmania in a domestic cat where amastigote forms of the parasite were observed in the popliteal lymph node imprint. Positive and negative serological reactions were observed by enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence assay (IFA), respectively. Polymerase chain reaction (PCR) revealed that the nucleotide sequence of the sample was identical to Leishmania (L.) chagasi. This is the first report of the disease in feline...

  6. Seroprevalence of Rickettsia bellii and Rickettsia felis in dogs, São José dos Pinhais, State of Paraná, Brazil Soroprevalência de Rickettsia bellii e Rickettsia felis em cães, São José dos Pinhais, Paraná, Brasil

    OpenAIRE

    Fernanda Silva Fortes; Iara Silveira; Jonas Moraes-Filho; Ronaldo Viana Leite; José Edivaldo Bonacim; Alexander Welker Biondo; Marcelo Bahia Labruna; Marcelo Beltrão Molento

    2010-01-01

    Brazilian spotted fever (BSF) is a vector-borne zoonosis caused by Rickettsia rickettsii bacteria. Dogs can be host sentinels for this bacterium. The aim of the study was to determine the presence of antibodies against Rickettsia spp. in dogs from the city of São José dos Pinhais, State of Paraná, Southern Brazil, where a human case of BSF was first reported in the state. Between February 2006 and July 2007, serum samples from 364 dogs were collected and tested at 1:64 dilutions by indirect i...

  7. Efeito de dois métodos de resfriamento sobre a funçao espermática in vitro de semen criopreservado de felinos (Leopardus tigrinus, Leopardus pardalis E Felis catus), avaliada através de ensaio competitivo de ligaçao em ovócitos de gata doméstica (Felis

    OpenAIRE

    Baudi, Daiam Loyola Kampa

    2005-01-01

    A busca de estratégias eficientes para a manutenção de espécies em risco de extinção tem impulsionado a comunidade científica a pesquisar alternativas para a preservação de material genético, com intuito de formação de bancos de genoma e aplicação de biotécnicas reprodutivas. Este estudo teve por objetivo avaliar os efeitos de dois protocolos diferentes de resfriamento pré-congelamento para a criopreservação de sêmen de jaguatirica (n=3), gato-do-mato-pequeno (n=4) e gato doméstíco (n=15). Ap...

  8. Manual de métodos parasitológicos e histopatológicos en piscicultura

    OpenAIRE

    Keim, Albert

    1982-01-01

    Recientes problemas provocados por epiemias de infecciones de Ichthyophthirius multifiliie en la cría de bagre negro (Rhandia sapo) cultivado por el Departamento de Acuicultura del INAPE de Uruguay, y el conocimiento de que todos los piscicultores tienen problemas con los parásitos, constituyeron la base y antecedente para el inicio de un programa de investigaciones parasitológicas. Este programa incluyó. 1) Disección de bagres capturados en Laguna de Sauce y colecta de parásitos. 2) Disecció...

  9. Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections in stray and pet cats (Felis catus) in northwest China: co-infections and risk factors.

    Science.gov (United States)

    Cong, Wei; Meng, Qing-Feng; Blaga, Radu; Villena, Isabelle; Zhu, Xing-Quan; Qian, Ai-Dong

    2016-01-01

    This study was conducted to estimate the prevalence of Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections among stray and pet cats in Lanzhou, northwest China, and to identify the influence of age, gender, and regions on seropositivity. T. gondii antibodies were examined in cat sera by the modified agglutination test (MAT). The circulating antigens of D. immitis and FeLV and specific antibodies to FIV were examined using kits commercially available. The overall prevalence of T. gondii, FIV, FeLV, and D. immitis was 19.34, 9.12, 11.33, and 3.04 %, respectively. For the genetic characterization of T. gondii genotypes in cats, genomic DNA was extracted from the seropositive cats and the T. gondii B1 gene was amplified using a semi-nested PCR. DNA samples giving positive B1 amplification were then genotyped using multilocus PCR-RFLP. Two T. gondii genotypes (ToxoDB#9 and ToxoDB#1) were identified. Results of the multivariate logistic regression analysis showed that older cats are more likely to be seropositive than juveniles for T. gondii, FIV, FeLV, and D. immitis. This is the first report of T. gondii genotypes in cats in northwest China. Moreover, the present study is the first study of retrovirus and D. immitis seroprevalence in cats in China. The results revealed that T. gondii, FIV, and FeLV infections are common in stray and pet cats in northwest China.

  10. DOENÇA DO TRATO URINÁRIO EM GATOS (Felis catus domesticus, LINNAEUS, 1758 ATENDIDOS EM CLÍNICAS VETERINÁRIAS DA REGIÃO DE RIBEIRÃO PRETO-SP

    Directory of Open Access Journals (Sweden)

    Letícia Neves

    2011-05-01

    Full Text Available O presente estudo teve como objetivos avaliar as afecções e os principais sinais clínicos em felinos domésticos com doença primária ou secundária do trato urinário (DTU e comparar os sintomasapresentados pelos animais com ou sem obstrução do trato urinário. Foram analisadas 49 fichas de pacientes felinos atendidos em 2 clínicas e 1 hospital veterinário, na região de Ribeirão Preto, SP. Os animais foram separados em grupos de acordo com a existência ou não de obstrução do fluxo urinário e se apresentavam sinaisclínicos referentes ao trato urinário secundários a outras doenças. O grupo G1 (animais obstruídos foi ainda subdividido em confirmados e suspeitos, o G2 (não obstruídos em confirmados, altamente suspeitos e suspeitos e o G3 foi constituído por felinos com manifestações do trato urinário secundárias à outras afecções. A doença do trato urinário (DTU foi verificada em 28 (57% machos e 21 fêmeas (43%. O G1 representou 39% das amostras (19 animais, o G2, 47% (23 animais e o G3, 14% (7 animais. Os sinais clínicos que diferiram entre os grupos G1 e G2 foram: oligúria, bexiga repleta / iscúria, poliúria, presença de plugs e urólitos no G1; piúria,sensibilidade na região renal, aumento de volume abdominal, icterícia, dificuldade de locomoção, gengivite e queda de pêlos no G2.This study aimed to evaluate the afeccion and clinical signs in domestic cats with primary or secondary disease of the urinary tract (DTU, and compare the symptoms shown by animals with or withouturinary tract obstruction. It has been analyzed 49 files of feline patients treated in two clinics and one veterinary hospital in the region of Ribeirão Preto, SP. The animals were separated into groups according to whether or not an obstruction of urinary tract was present, and if clinical signs related to the urinary tract were found. The G1 (obstructed animals was further divided into confirmed and suspected, G2 (non obstructed in confirmed,suspected and highly suspicious, and G3 was made up of cats with urinary tract manifestations secondary to other diseases. The urinary tract disease (DTU was seen in being 28 (57% males and 21 females (43%. The G1 accounted for 39% of samples (19 animals, G2 47% (23 animals, and G3, 14% (7 animals. The clinical signs that differed between groups G1 and G2 were: oliguria, full bladder / urinary retention, polyuria, presence of plugs and uroliths in G1; pyuria, renal sensitivity in the region, abdominal swelling, jaundice, locomotion difficulty, gingivitis and falling hairs in G2.

  11. Efecto del fotoperíodo y de la administración de melatonina sobre la producción espermática en el gato doméstico (Felis silvestris catus)

    OpenAIRE

    Núñez Favre, Romina de los Ángeles

    2013-01-01

    En el felino doméstico, la estacionalidad ovulatoria y estral de la hembra ocurre durante los días que presentan más de 12 h luz. Sin embargo, la estacionalidad reproductiva del gato ha sido definida recientemente. El objetivo de esta tesis fue estudiar el efecto del fotoperiodo natural, el manejo lumínico artificial y la administración de melatonina sobre la producción espermática en el gato doméstico. En el primer estudio se evaluó el efecto del fotoperiodo natural sobre la morfología testi...

  12. DETECTION OF RICKETTSIA FELIS LIKE AGENT FROM EULAELAPS STABULARIS KOCH%从厩真厉螨中检出与猫立克次体近缘的立克次体核酸片段

    Institute of Scientific and Technical Information of China (English)

    姚李四; 刘勇先; 邵丽筠; 张晓龙; 杨宇; 王静; 徐宝梁; 王宝麟; 赵彤言

    2012-01-01

    本研究用立克次体属特异的gltA和ompB基因扩增引物,从吉林长白县捕获鼠中分拣的534只厩真厉螨中扩得gltA和ompB基因片段.通过基因片段的序列测定、BLAST比对和系统发育分析,显示两个扩增基因与猫立克次体Rickettsia felis同源性最高(99%),证明该地区厩真厉螨携带与猫立克次体近缘的立克次体.%The segments of glt A gene and omp B gene were amplified and identified with Rickettsia specific git A and omp B primers from 534 mites (Eulaelaps stabularis Koch) collected from rodents of Changbai County, Jilin Province, China. By sequencing, BLAST qualifying and phylogenetic analyzing, it was revealed that the two amplified segments were of highest homology to Rickettsia fells (99% ) , implying that the locally collected E. Stabularis may carry some Rickettsia sp. Allied to Rickettsia fells.

  13. Dicty_cDB: CHH715 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available M056853.1 2154-85 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 215...an tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2191-29, mRNA sequence. 90 1e-13 1 BM0585...78 |BM058578.1 2193-94 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clon

  14. Dicty_cDB: SHA256 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available subtracted cDNA library Ctenocephalides felis cDNA clone 2154-85, mRNA sequence. 92 3e-14 1 DR447725 |DR4477...94 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cD...acted cDNA library Ctenocephalides felis cDNA clone 2191-29, mRNA sequence. 90 1e-13 1 dna update 2005.12. 5

  15. Parasites of domestic and wild animals in South Africa. XLIV. Fleas (Insecta : Siphonaptera : Pulicidae collected from 15 carnivore species

    Directory of Open Access Journals (Sweden)

    I.G. Horak

    2004-11-01

    Full Text Available Fleas were collected from 61 wild carnivores belonging to 13 species in various nature reserves and on farms, two feral domestic cats in a nature reserve and a domestic dog in the city of Johannesburg. Eleven flea species, including two subspecies of one of these, belonging to six genera were recovered. Amongst these only Ctenocephalides felis felis and Ctenocephalides felis strongylus are considered specific parasites of carnivores. The remaining ten species normally infest the prey animals of the various carnivores.

  16. Monogenoidea (Polyonchoinea: Dactylogyridae) parasitizing the gills of marine catfish (Siluriformes: Ariidae) inhabiting the Atlantic Amazon Coast of Brazil.

    Science.gov (United States)

    Domingues, Marcus V; Soares, Geusivam B; Watanabe, Alana

    2016-06-23

    A parasitological survey of monogenoids infesting the gills of marine catfish (Ariidae) captured from the Atlantic coastal region of the Amazon Basin was carried out during the 2011-2013 period. The gills of 448 specimens involving twelve ariid species (29 Amphiarius rugispinis (Valenciennes), 52 Aspistor quadriscutis (Valenciennes), 74 Bagre bagre (Linnaeus), 16 Cathorops arenatus (Valenciennes), 13 Cathorops agassizii (Eigenmann & Eigenmann), 17 Cathorops spixii (Agassiz), 3 Cathorops sp., 13 Notarius grandicassis (Valenciennes), 14 Sciades couma (Valenciennes), 64 Sciades herzbergii (Bloch), 48 Sciades parkeri (Traill), 13 Sciades passany (Valenciennes), 92 Sciades proops (Valenciennes) were sampled. No monogenoids were found in Cathorops agassizi, Cathorops arenatus, Cathorops spixii, Cathorops sp. and Sciades parkeri, but the gills of the other sampled species were parasitized by at least one species of monogenoid. We identified four new species of Chauhanellus and one new species of Hamatopeduncularia: Chauhanellus hamatopeduncularoideum n. sp. from Amphiarius rugispinis and Sciades couma; Chauhanellus hypenocleithrum n. sp. from Sciades proops; Chauhanellus susamlimae n. sp. from Sciades herzbergii and Sciades passany; Chauhanellus velum n. sp. from Sciades couma, Sciades herzbergii and Sciades passany; and Hamatopeduncularia cangatae n. sp. from Aspistor quadriscutis and Notarius grandicassis. Four previously described species were reported for the first time parasitizing ariids from Atlantic Amazon: Chauhanellus neotropicalis Domingues & Fehlauer, 2006 from Amphiarius rugispinis, Aspistor quadriscutis, Notarius grandicassis and Sciades passany; Chauhanellus boegeri Domingues & Fehlauer, 2006 from Sciades couma and Sciades herzbergii; Hamatopeduncularia bagre Hargis, 1955 from Bagre bagre; and Neomurraytrematoides proops Zambrano & Añez 1993 from Sciades passany. The monotypic Neomurraytrematoides Zambrano & Añez 1993 was placed in synonymy with

  17. NCBI nr-aa BLAST: CBRC-SARA-01-0195 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-SARA-01-0195 ref|NP_001009331.1| cannabinoid receptor 1 [Felis catus] sp|O02777|CNR1_FELCA Canna...binoid receptor 1 (CB1) (CB-R) gb|AAB53440.1| CB1 cannabinoid receptor [Felis catus] NP_001009331.1 0.0 96% ...

  18. NCBI nr-aa BLAST: CBRC-RNOR-05-0081 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-05-0081 ref|NP_001009331.1| cannabinoid receptor 1 [Felis catus] sp|O02777|CNR1_FELCA Canna...binoid receptor 1 (CB1) (CB-R) gb|AAB53440.1| CB1 cannabinoid receptor [Felis catus] NP_001009331.1 0.0 96% ...

  19. NCBI nr-aa BLAST: CBRC-FCAT-01-1020 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FCAT-01-1020 ref|NP_001009331.1| cannabinoid receptor 1 [Felis catus] sp|O02777|CNR1_FELCA Canna...binoid receptor 1 (CB1) (CB-R) gb|AAB53440.1| CB1 cannabinoid receptor [Felis catus] NP_001009331.1 0.0 98% ...

  20. NCBI nr-aa BLAST: CBRC-CFAM-12-0016 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CFAM-12-0016 ref|NP_001009331.1| cannabinoid receptor 1 [Felis catus] sp|O02777|CNR1_FELCA Canna...binoid receptor 1 (CB1) (CB-R) gb|AAB53440.1| CB1 cannabinoid receptor [Felis catus] NP_001009331.1 0.0 97% ...

  1. NCBI nr-aa BLAST: CBRC-TBEL-01-1883 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TBEL-01-1883 ref|NP_001009331.1| cannabinoid receptor 1 [Felis catus] sp|O02777|CNR1_FELCA Canna...binoid receptor 1 (CB1) (CB-R) gb|AAB53440.1| CB1 cannabinoid receptor [Felis catus] NP_001009331.1 0.0 97% ...

  2. Dicty_cDB: SLJ752 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available dgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2193-94, mRNA sequence. ...acted cDNA library Ctenocephalides felis cDNA clone 2191-29, mRNA sequence. 60 1e-05 1 CA916753 |CA916753.1

  3. Dicty_cDB: CHE836 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone... 2154-85, mRNA sequence. 92 2e-14 1 BM058578 |BM058578.1 2193-94 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalide...hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA

  4. Considerações sobre pulgas (Siphonaptera da raposa Cerdocyon thous (Canidae da área endêmica de leishmaniose visceral de Jacobina, Bahia, Brasil

    Directory of Open Access Journals (Sweden)

    Cerqueira Elúzio J.L.

    2000-01-01

    Full Text Available No período de julho a setembro de 1998 foram coletadas 152 pulgas em 18 exemplares da raposa Cerdocyon thous capturados na área endêmica de leishmaniose visceral de Jacobina, Estado da Bahia. As pulgas foram identificadas como: 136 Rhopalopsyllus lutzi lutzi, 11 Pulex irritans, 2 Ctenocephalides canis, 1 Ctenocephalides felis felis e 2 Xenopsylla cheopis.

  5. Avaliação do jundiá (Rhamdia quelen em diferentes sistemas de cultivo para a região do litoral centro norte de Santa Catarina, Brazil

    Directory of Open Access Journals (Sweden)

    Hilton Amaral Junior

    2008-12-01

    Full Text Available El bagre (Rhamdia quelen, un pez nativo habitante de la mayoría de los ríos, lagos y lagunas de Santa Catarina, y que pertenecen a la orden Siluriformes, familia Pimelodidae, género Rhandia. El estudio tiene como objetivo evaluar el desempeño de este pez nativo en los sistemas más utilizados en Santa Catarina. El experimento se llevó a cabo en el ámbito del Campo Experimental de Piscicultura de Camboriú CEPC-EPAGRI, cuando 11 tanques excavados fueron utilizados, tres en representación de un monocultivo de bagre con una densidad de 1,5 p/m2, tres en representación de un bicultivo de bagre y tilapia con una densidad de 3p/m2, tres en representación de un policultivo de la carpa, tilapia y "jundiás" con una densidad de 3p/m2, y en dos tanques excavados fueron instalados seis tanques redes, en representación de las culturas de alta densidad de 50 y 100 p/m3.

  6. Dicty_cDB: SHH217 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 16 3 BM056853 |BM056853.1 2154-85 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides feli...9 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDN...2018A03 5', mRNA sequence. 88 6e-14 2 BM058578 |BM058578.1 2193-94 hindgut and Malpighian tubule subtracted cDNA library Ctenocephali...des felis cDNA clone 2193-94, mRNA sequence. 90 1e-13 1 BM058379 |BM058379.1 2191-2

  7. Dicty_cDB: AHA269 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 853 |BM056853.1 2154-85 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clo...BM058379 |BM058379.1 2191-29 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDN...ne 2154-85, mRNA sequence. 92 2e-14 1 BM058578 |BM058578.1 2193-94 hindgut and Malpighian tubule subtracted cDNA library Ctenocephali...des felis cDNA clone 2193-94, mRNA sequence. 90 8e-14 1

  8. Dicty_cDB: Contig-U11103-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available hamii 37, com... 127 2e-27 Y18740_1( Y18740 |pid:none) Gracilariopsis lemaneiformis Agll4 gene. 127 3e-27 AX...ariopsis lemaneiformis Agll2 gene. 121 2e-25 Y18737_1( Y18737 |pid:none) Gracilar..... 108 2e-21 Y18741_1( Y18741 |pid:none) Gracilariopsis lemaneiformis Agll5 gen... 107 3e-21 AY229885_1( AY2... sequence from clone RP11... 68 2e-09 Y18739_1( Y18739 |pid:none) Gracilariopsis lemaneiformis Agll3 gen... ...CC 13... 121 1e-25 CR378676_14( CR378676 |pid:none) Photobacterium profundum SS9 chro... 121 2e-25 Y18738_1( Y18738 |pid:none) Gracil

  9. Tõsõ mu maailman : [luuletused] / Häniläne, pseud.

    Index Scriptorium Estoniae

    Häniläne,, pseud.

    2008-01-01

    Sisu: Bombus lucorum (maakimalane) ; Podiceps auritus (sarvikpütt) ; Malus domestica (aed-õunapuu) ; Felis catus domesticus (kodukass) ; Inachis io (päevapaabusilm) ; Lacerta vivipara (arusisalik) ; Canis familiaris (kodukoer) ; Clangula hyemalis (aul)

  10. 75 FR 74740 - Measure M2 Natural Community Conservation Plan/Habitat Conservation Plan/Master Streambed...

    Science.gov (United States)

    2010-12-01

    ... proposing to cover 17 (4 listed and 13 unlisted) animal species and 5 plant species (1 listed and 4 unlisted... chub (Gila orcuttii), (12) bobcat (Lynx rufus), (13) mountain lion (Felis concolor), (14) pallid...

  11. Mercury contamination in Florida panthers [Draft

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — As a result of the death of an apparently healthy, radio-collared female Florida panther (Felis concolor qoryi) (FP#27) in Everglades National Park in July 1989, we...

  12. CROSS TRANSMISSION OF 'GIARDIA'

    Science.gov (United States)

    Giardia cysts isolated from fecal samples obtained from humans (Homo sapiens), beaver (Castor canadensis), dogs (Canis familiaris), cats (Felis domesticus), bighorn X mouflon sheep (Ovis canadensis X O. musimon), guinea pig (Cavis porcellus), muskrat (Ondatra ziethica) and mule d...

  13. Dicty_cDB: VHD773 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 853.1 2154-85 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2154-85...uence. 88 7e-14 2 BM058578 |BM058578.1 2193-94 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides...hian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2191-29, mRN

  14. Dicty_cDB: AFK408 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available d Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2154...-85, mRNA sequence. 92 1e-14 1 BM058578 |BM058578.1 2193-94 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides...ut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2191-29, mRNA sequence. 90

  15. Dicty_cDB: VSG402 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available M058328 |BM058328.1 2189-54 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA...d cDNA library Ctenocephalides felis cDNA clone 2163-12, mRNA sequence. 42 3.7 1 ... clone 2189-54, mRNA sequence. 42 3.7 1 BM057177 |BM057177.1 2163-12 hindgut and Malpighian tubule subtracte

  16. The Carnivores of the Northeastern Badia, Jordan

    OpenAIRE

    BUNAIAN, Fayez

    2001-01-01

    The presence of 8 carnivores representing 3 families (Canidae, Felidae and Hyaenidae) in the northeastern Badia was con-firmed by trapping and spotlighting. The family Canidae is represented by 4 species: Canis aureus syriaca, Canis lupus arabs, Vulpes vulpes and Vulpes rueppelli. Three felines, Caracal caracal schmitzi, Felis margarita and Felis sylvestris tristrami, were spotlighted. Remains of recently killed Hyaena hyaena syriacawere recovered. Major threats affecting the population of d...

  17. Haemobartonellosis in Van Cats

    OpenAIRE

    AKKAN, Hasan Altan; Karaca, Mehmet; TÜTÜNCÜ, Mehmet

    2005-01-01

    The present study was conducted to determine prevalence of Haemobartonella felis in Van cats. 121 Van cats (82 female, 39 male, aged 1-9 years) were the materials of the study. To determine biochemical and haematological parameters, 2 ml blood with and without anticoagulant were taken according to technique from vena cephalica antebrachii. H. felis was detected in blood smears preparations of 18 (14.88%) by Papenheim staining. Among biochemical parameters aspartate amino transferase (AST), al...

  18. Transmission mechanisms of an emerging insect-borne rickettsial pathogen

    OpenAIRE

    Brown, Lisa D.; Kaikhushroo H Banajee; Lane D Foil; Macaluso, Kevin R.

    2016-01-01

    Background Vector-borne pathogens must overcome arthropod infection and escape barriers (e.g. midgut and salivary glands) during the extrinsic incubation period (EIP) before subsequent transmission to another host. This particular timespan is undetermined for the etiological agent of flea-borne spotted fever (Rickettsia felis). Artificial acquisition of R. felis by blood-feeding cat fleas revealed dissemination to the salivary glands after seven days; however, this length of time is inconsist...

  19. Caratterizzazione biometrica di popolazioni selvatiche di gatto selvatico sardo

    OpenAIRE

    Murgia, Carlo; Murgia, Andrea; Deiana, Anna Maria

    2005-01-01

    This paper is about the variation in body size and cranial dimensions in natural population of the Sardinian wildcat. We have recorded biometric measures of 40 wildcats (24 male and 16 female) collected or captured in different localities of Sardinia. Craniometrical measures were taken on seven of these cats. The results obtained in this study indicate that the Sardinian wildcat is smaller than the European (Felis silvestris silvestris) and the African (Felis silvestris libyca) wildcat. Crani...

  20. A comparative study on electrochemical cycling stability of lithium rich layered cathode materials Li1.2Ni0.13M0.13Mn0.54O2 where M = Fe or Co

    Science.gov (United States)

    Laisa, C. P.; Nanda Kumar, A. K.; Selva Chandrasekaran, S.; Murugan, P.; Lakshminarasimhan, N.; Govindaraj, R.; Ramesha, K.

    2016-08-01

    In this work we compare electrochemical cycling stability of Fe containing Li rich phase Li1.2Ni0.13Fe0.13Mn0.54O2 (Fe-Li rich) with the well-known Co containing Li rich composition Li1.2Ni0.13Co0.13Mn0.54O2 (Co-Li rich). During the first charge, the activation plateau corresponding to removal of Li2O from the structure is smaller (removal of 0.6 Li) in the case of Fe-Li rich compared to Co-Li rich composition (0.8 Li removal). Consequently, the Fe compound shows better capacity retention; for example, after 100 cycles Fe-Li rich compound exhibits 20% capacity degradation where as it is about 40% in the case of Co-Li rich phase. The electrochemical and microscopy studies support the fact that compared to Co-Li rich compound, the Fe-Li rich composition display smaller voltage decay and reduced spinel conversion. XPS studies on charged/discharged Fe-Li rich samples show participation of Fe+3/Fe+4 redox during electrochemical cycling which is further supported by our first principles calculations. Also the temperature dependent magnetic studies on charge-discharged samples of Fe-Li rich compound point out that magnetic behavior is sensitive to cation oxidation states and Ni/Li disorder.

  1. Detection of Bartonellaspp. and Rickettsiaspp. in fleas, ticks and lice collected in rural areas of Peru

    Directory of Open Access Journals (Sweden)

    Abraham G. Cáceres

    2013-12-01

    Full Text Available Bartonellosis and rickettsiosis are commonly reported in Peru. In order to detect Bartonella sp. and Rickettsiasp. in fleas, ticks and lice, specimens from five distinct locations in Peru (Marizagua, Cajaruro, Jamalca, Lonya Grande and El Milagro were collected and screened for the presence of these bacteria using PCR and later confirmation by DNA sequencing. The specimens collected were distributed in 102 pools (76 Ctenocephalides felis, 2 Ctenocephalides canis, 16 Pulex irritans, 5 Pediculus humanus, 2 Rhiphicephalus sanguineus, and 1 Boophilus spp., where Bartonellawas detected in 17 pools (6 of C. felis, 9 of P. irritans, 1 of C. canis, and 1 P. humanus. Also, Rickettsiawas detected in 76 pools (62 C. felis, 10 P. irritans, 2 P. humanus, and 2 C. canis. Bartonella clarridgeiaewas detected in C. felis, C. canisand P. irritanspools at 5.3%, 50% and 12.5%, respectively.Bartonella rochalimaewas detected in one C. felisand two P. irritanspools at 1.3% and 12.5%, respectively. Furthermore, B. henselaewas detected in one C. felispool and one P. humanuspool corresponding to 1.3% and 20%, respectively; and Bartonella spp.was also found in 5 pools of P. irritansat 31.3%. Additionally, R. feliswas detected in C. felis, C. canisand P. irritanspools at 76.3%, 100% and 37.5%, respectively; and Rickettsia spp. was detected in C. felis, P. irritansand P. humanuspools at 5.3%, 25% and 40%, respectively. These results demonstrate the circulation of these bacteria in Peru.

  2. Differential Rickettsial Transcription in Bloodfeeding and Non-Bloodfeeding Arthropod Hosts

    Science.gov (United States)

    Verhoeve, Victoria I.; Jirakanwisal, Krit; Utsuki, Tadanobu; Macaluso, Kevin R.

    2016-01-01

    Crucial factors influencing the epidemiology of Rickettsia felis rickettsiosis include pathogenesis and transmission. Detection of R. felis DNA in a number of arthropod species has been reported, with characterized isolates, R. felis strain LSU and strain LSU-Lb, generated from the cat flea, Ctenocephalides felis, and the non-hematophagous booklouse, Liposcelis bostrychophila, respectively. While it is realized that strain influence on host biology varies, the rickettsial response to these distinct host environments remained undefined. To identify a panel of potential rickettsial transmission determinants in the cat flea, the transcriptional profile for these two strains of R. felis were compared in their arthropod hosts using RNAseq. Rickettsial genes with increased transcription in the flea as compared to the booklouse were identified. Genes previously associated with bacterial virulence including LPS biosynthesis, Type IV secretion system, ABC transporters, and a toxin-antitoxin system were selected for further study. Transcription of putative virulence-associated genes was determined in a flea infection bioassay for both strains of R. felis. A host-dependent transcriptional profile during bloodfeeding, specifically, an increased expression of selected transcripts in newly infected cat fleas and flea feces was detected when compared to arthropod cell culture and incubation in vertebrate blood. Together, these studies have identified novel, host-dependent rickettsial factors that likely contribute to successful horizontal transmission by bloodfeeding arthropods. PMID:27662479

  3. Utilização experimental de hidroxiapatita sintética em alvéolos dentários de gatos domésticos (Felis catus: estudo clínico, radiográfico e histomorfométrico

    Directory of Open Access Journals (Sweden)

    E.C. Silva

    2012-08-01

    Full Text Available A fim de avaliar a resposta biológica da hidroxiapatita sintética (HAP-91 nos alvéolos de felinos domésticos, este biomaterial foi implantado após extração do terceiro pré-molar inferior direito em 12 gatos e mantida por meio de uma membrana de celulose bacteriana. No lado esquerdo, os alvéolos foram apenas recobertos com a membrana de celulose bacteriana, formando o grupo-controle. Observou-se, durante a avaliação clínica, que todos os animais voltaram a comer normalmente ração úmida, sem apresentarem sinais de dor ou desconforto após a recuperação anestésica. A cicatrização da ferida cirúrgica ocorreu de forma satisfatória, sendo que a membrana de celulose bacteriana evitou a saída precoce da hidroxiapatita. Radiograficamente, aos 50 dias, todos os animais apresentaram radiopacidade óssea homogênea em ambos os lados. À análise histomorfométrica, observou-se adiantamento do processo de reparo do osso alveolar nos oito primeiros dias do grupo-tratado quando comparado ao grupo-controle, bem como atraso aos 30 dias, porém, aos 50 dias, ambos os grupos apresentavam porcentagem de tecido ósseo semelhante e morfologicamente normal. Os resultados sugerem que a hidroxiapatita é biocompatível, integra-se ao tecido ósseo alveolar e pode ser utilizada em felinos.

  4. Ankara Kedilerinde (Felis catus angorensis) dış yapı, tüy, büyüme, gelişme ve üreme özellikleri üzerine araştırmalar

    OpenAIRE

    ÖZÇETİN, Sebahattin Tarkan

    2007-01-01

    Ankara Atatürk Orman Çiftliği (A.O.Ç.) Hayvanat Bahçesinde yetiştirilen 5 erkek, 28 dişi ve bu kedilerden doğan 70  yavru  ile  Ankara’nın  çeşitli  semtlerinde  bakılan  11  erkek, 10 dişi, 4 kastre erkek ve 9 steril dişi olmak üzere toplam 137 Ankara Kedisinin kullanıldığı   çalışmada  Ankara Kedisinin dış yapı, tüy, büyüme, gelişme ve üreme özellikleri incelenmiştir. Ankara A.O.Ç. Ha...

  5. MAST CELLS, MAST/STEM CELL GROWTH FACTOR RECEPTOR (C-KIT/CD117 AND IGE MAY BE INTEGRAL TO THE PATHOGENESIS OF ENDEMIC PEMPHIGUS FOLIACEUS

    Directory of Open Access Journals (Sweden)

    Ana Maria Roselino

    2013-11-01

    Full Text Available Introduction: Pemphigus foliaceus (PF is endemic in some South American countries, especially in Colombia and Brazil; in Brazil, it is also known as fogo selvagem (FS. We aimed to study the presence of mast cells and the expression of the mast/stem cell growth factor receptor (c-kit/CD117 in PF skin biopsies, as well as the role of IgE in the disease pathogenesis. Methods: Forty-four skin biopsies from patients affected by endemic PF (EPF (30 patients from El Bagre, Colombia, and 14 from the northeastern region of São Paulo State, Brazil, 48 control biopsies from Colombian and Brazilian endemic areas, and additional control biopsies from none endemic areas in Colombia and the USA non were studied. Immunohistochemistry (IHC was performed to evaluate skin biopsies with anti-mast cell tryptase (MCT, anti-c-kit and anti-IgE antibodies. We also searched for serum IgE in 30 EPF and 30 non-atopic controls from the El Bagre region via ELISA. In our El Bagre patients and controls, we also searched for IgE in skin samples by direct immunofluorescence. Results: In 100% of the EPF biopsies, MCT, c-kit and IgE were identified with stronger expression relative to control biopsies, especially in the inflammatory infiltrates around upper dermal blood vessels and dermal eccrine glands. IgE staining was positive along the BMZ in some EPF skin samples. The DIF results confirmed a linear deposition of IgE at the BMZ. Increased IgE serum levels were also noted in PF patients relative to controls.. Conclusions: In patients with EPF, the observed increased expression of MCT, c-kit and IgE in lesional skin, associated with higher serum IgE levels may indicate possible IgE participation in the antigenic response.

  6. Endemic pemphigus over a century: Part II

    Directory of Open Access Journals (Sweden)

    Ana María Abréu-Vélez

    2010-03-01

    Full Text Available Background: Endemic pemphigus foliaceus (EPF is an autoimmune disease, classically occurring in a restricted geographic area. Foci of EPF have been described in several Central and South American countries, often affecting young people and Amerindians, with some female predilection. Although most American EPF cases have been documented in Brazil, cases have been reported in Peru, Paraguay, El Salvador and Venezuela. An additional variant of EPF has been described in El Bagre, Colombia, (El Bagre-EPF affecting older men and a few post-menopausal females. Finally, one additional type of EPF has been described in nomadic tribes affecting females of child bearing age in Tunisia, Africa. Aims: The main aim of this review is to summarize current knowledge about autoantigens, and immunologic and genetic studies in EPF. Material and Methods: We utilized a retrospective review of the literature, aiming to compile and compare the multiple geographic foci of EPF. Results: The primary autoantigens in EPF are still considered to be desmogleins in the case of the Tunisian and all American cases, in contradistinction to plakins and desmogleins in El Bagre-EPF. Although several autoantigens are been suggested, their biochemical nature needs further elucidation. Current knowledge still supports the concept that an antibody mediated immune response represents the principal pathophysiology in all variants of EPF. Conclusion: A strong genetic susceptibility appears to contribute to disease development in several people affected by these diseases; however, no specific genes have been confirmed at present. We conclude that further investigation is necessary to define these disorders immunologically and genetically.

  7. Endemic pemphigus over a century: Part II

    Directory of Open Access Journals (Sweden)

    Ana María Abréu-Vélez

    2010-01-01

    Full Text Available Background : Endemic pemphigus foliaceus (EPF is an autoimmune disease, classically occurring in a restricted geographic area. Foci of EPF have been described in several Central and South American countries, often affecting young people and Amerindians, with some female predilection. Although most American EPF cases have been documented in Brazil, cases have been reported in Peru, Paraguay, El Salvador and Venezuela. An additional variant of EPF has been described in El Bagre, Colombia, (El Bagre-EPF affecting older men and a few post-menopausal females. Finally, one additional type of EPF has been described in nomadic tribes affecting females of child bearing age in Tunisia, Africa. Aims: The main aim of this review is to summarize current knowledge about autoantigens, and immunologic and genetic studies in EPF. Material and Methods: We utilized a retrospective review of the literature, aiming to compile and compare the multiple geographic foci of EPF. Results: The primary autoantigens in EPF are still considered to be desmogleins in the case of the Tunisian and all American cases, in contradistinction to plakins and desmogleins in El Bagre-EPF. Although several autoantigens are been suggested, their biochemical nature needs further elucidation. Current knowledge still supports the concept that an antibody mediated immune response represents the principal pathophysiology in all variants of EPF. Conclusion : A strong genetic susceptibility appears to contribute to disease development in several people affected by these diseases; however, no specific genes have been confirmed at present. We conclude that further investigation is necessary to define these disorders immunologically and genetically.

  8. Evaluation of the effectiveness of a novel oral formulation of sarolaner (Simparica™) for the treatment and control of fleas on dogs.

    Science.gov (United States)

    Six, Robert H; Geurden, Thomas; Packianathan, Raj; Colgan, Sally; Everett, William R; Grace, Sarah; Hodge, Andrew; Mahabir, Sean P; Myers, Melanie R; Slootmans, Nathalie; Davis, Kylie

    2016-05-30

    The efficacy of a single oral dose of a novel isoxazoline, sarolaner (Simparica™, Zoetis), for the treatment and control of flea infestations on dogs was confirmed in five laboratory studies. The studies were conducted using adult purpose-bred Beagles and/or mixed breed dogs. All animals were individually identified and housed, and were allocated randomly to treatment with either placebo or sarolaner (eight to 10 per group) based on pretreatment parasite counts. Three studies used cat flea (Ctenocephalides felis felis) strains recently isolated from the field from the US, EU, or Australia; in the fourth study a laboratory strain (KS1) with documented tolerance to a number of insecticides such as fipronil, imidacloprid, and permethrin was used. In the fifth study, dogs were infested with dog fleas, Ctenocephalides canis. Dogs were treated orally on Day 0 with a placebo or a sarolaner tablet providing a minimum dose of 2mg/kg. Dogs were infested with approximately 100 unfed, adult fleas prior to treatment and at weekly intervals post-treatment. Comb counts were conducted to determine the numbers of viable fleas at 24h after treatment and after each subsequent infestation. Efficacy against C. felis and C. canis was 99.8-100% from treatment through Day 35. In all five studies, elimination of existing infestations was achieved within 24h after dosing, with only a single live C. felis found on one dog on Day 1. Similarly, control of flea challenges was achieved within 24h after infestation throughout the 35day study periods, with only single live C. felis found on two dogs on Day 28 in one study, and on a single dog on Day 35 in another study. There were no adverse reactions to treatment with sarolaner. These studies confirmed that a single oral dose of sarolaner at 2mg/kg provided highly effective treatment of existing C. felis infestations and persistent control of C. felis on dogs for 35days after treatment. Efficacy equivalent to that seen with C. felis was

  9. Determination of the prevalence of Helicobacter heilmannii-like organisms type 2 (HHLO-2) infection in humans and dogs using non-invasive genus/species-specific PCR in Korea.

    Science.gov (United States)

    Chung, Tae-Ho; Kim, Hee-Dong; Lee, Young-Sun; Hwang, Cheol-Yong

    2014-01-01

    Helicobacter spp. may have multiple routes of transmission. It is unclear, however, whether the agent is zoonotic and therefore transmitted from an animal reservoir, including dogs. The aim of this population-based study was to assess the relationship between pet ownership or frequent exposure to dogs and Helicobacter spp. infection, especially focusing on HHLO-2 (Helicobacter heilmannii-like organisms type 2) in saliva and feces samples in Korea, using non-invasive genus/species-specific PCR. One hundred twenty-four eligible human subjects and 39 dogs participated in this study. Relativity of contact with dogs and Helicobacter spp. infection diagnosed by genus-specific PCR showed a statistically significant result (Pdogs and H. pylori, H. felis and H. bizzozeronii infections diagnosed using species-specific PCR, only Helicobacter felis showed a statistically significant result. Although H. pylori infection showed a statistically significant relativity, no statistically significant association was found between veterinarian subjects and Helicobacter. spp., H. felis and H. bizzozeronii infections. On performing risk factor analyses of HHLO-2 infection by transmission, using matching species, between HHLO-2-positive dog owners and HHLO-2-positive dogs, Helicobacter felis infection showed an extremely significant relativity (Pdogs was proved to be correlated with human H. felis and H. bizzozeronii infections in this study. PMID:24065079

  10. Modelling growth-competition relationships in trembling aspen and white spruce mixed boreal forests of Western Canada.

    Directory of Open Access Journals (Sweden)

    Jian-Guo Huang

    Full Text Available We examined the effect of competition on stem growth of Picea glauca and Populus tremuloides in boreal mixedwood stands during the stem exclusion stage. We combined traditional approaches of collecting competition data with dendrochronology to provide retrospective measurements of stem diameter growth. Several competition indices including stand basal area (BA, the sum of stem diameter at breast height (SDBH, and density (N for the broadleaf and coniferous species, as well as similar indices considering only trees with diameters greater than each subject (BAGR, SDBHGR, and NGR, were evaluated. We used a nonlinear mixed model to characterize the basal area increment over the past 5, 10, 15, 20, 25, 30, and 35 years as a function of growth of nearby dominant trees, the size of the subject trees, deciduous and coniferous competition indices, and ecoregions. SDBHGR and BAGR were better predictors for spruce, and SDBHGR and NGR were better for aspen, respectively, than other indices. Results showed strongest correlations with long-term stem growth, as the best models integrated growth for 10-25 years for aspen and ≥ 25 for spruce. Our model demonstrated a remarkable capability (adjusted R(2>0.67 to represent this complex variation in growth as a function of site, size and competition.

  11. Modelling growth-competition relationships in trembling aspen and white spruce mixed boreal forests of Western Canada.

    Science.gov (United States)

    Huang, Jian-Guo; Stadt, Kenneth J; Dawson, Andria; Comeau, Philip G

    2013-01-01

    We examined the effect of competition on stem growth of Picea glauca and Populus tremuloides in boreal mixedwood stands during the stem exclusion stage. We combined traditional approaches of collecting competition data with dendrochronology to provide retrospective measurements of stem diameter growth. Several competition indices including stand basal area (BA), the sum of stem diameter at breast height (SDBH), and density (N) for the broadleaf and coniferous species, as well as similar indices considering only trees with diameters greater than each subject (BAGR, SDBHGR, and NGR), were evaluated. We used a nonlinear mixed model to characterize the basal area increment over the past 5, 10, 15, 20, 25, 30, and 35 years as a function of growth of nearby dominant trees, the size of the subject trees, deciduous and coniferous competition indices, and ecoregions. SDBHGR and BAGR were better predictors for spruce, and SDBHGR and NGR were better for aspen, respectively, than other indices. Results showed strongest correlations with long-term stem growth, as the best models integrated growth for 10-25 years for aspen and ≥ 25 for spruce. Our model demonstrated a remarkable capability (adjusted R(2)>0.67) to represent this complex variation in growth as a function of site, size and competition.

  12. Ecological knowledge and incidental capture of sea turtles in São João de Pirabas, Pará, Brazil

    Directory of Open Access Journals (Sweden)

    Tiago Pereira Brito

    2015-09-01

    Full Text Available This study aimed to register the ecological knowledge of fishermen from the municipality of São João de Pirabas, Pará, Brazil, regarding the occurrence of sea turtles on the Pará state coast, as well as measure their incidental capture when fishing; to do this, 50 semi-structured interviews were conducted with local fishermen. Fishing was practiced mostly by adult men, who used 7 fishing arts (gillnetting, hook and line, longline, fish corrals, net of tide canals, casting net, and basket trap, mainly aimed at catching king mackerel (Scomberomorus cavalla, serra Spanish mackerel (S. brasiliensis, king weakfish (Macrodon ancylodon, weakfish (Cynoscion spp., hake (Cynoscion spp., catfish (Bagre bagre, and mullet (Mugil spp.. Fishermen observed in the region 5 turtle species, with a more frequent occurrence of Chelonia mydas (100%, Dermochelys coriacea (66%, and Eretmochelys imbricata (46%; the less frequent species are Caretta caretta (16% and Lepidochelys olivacea (8%. The spawning areas of the 3 most common species demonstrate the significance of the Pará state coast for their conservation. Incidental capture was reported by 76% of fishermen, mainly occurring in net, longlines, and fish corrals. Usually, captured animals were released, although there is consumption of sea turtle meat and eggs by fishermen.

  13. Isolation and identification of Helicobacter spp, from canine and feline gastric mucosa

    DEFF Research Database (Denmark)

    Jalava, K.; On, Stephen L.W.; VanDamme, P.A.R.;

    1998-01-01

    It is known that virtually all healthy adult dogs and cats harbor spiral helicobacters in their gastric mucosa, Three species, Helicobacter felis, Helicobacter bizzozeronii, and Helicobacter salomonis have been isolated in vitro from the gastric mucosa of these animals. The aims of this study were...... conventional phenotypic tests, whole-cell protein profiling, and ultrastructural analysis in identifying the different species isolated from canine and feline gastric mucose. We cultured 95 and 22 gastric mucosal biopsies from dogs and cats, respectively. Twenty-one H. bizzozeronii strains, 8 H. felis strains......, 8 H. salomonis strains, 3 mixed cultures, 2 "Flexispira rappini"-like organisms, and 3 as get uncharacterized strains were isolated from the dogs, and 3 H. felis strains were isolated from the cats. The methods used here yielded Helicobacter isolation rates of 51% from dogs and 13.6% from cats...

  14. How cats lap

    Science.gov (United States)

    Stocker, Roman; Reis, Pedro; Jung, Sunghwan; Aristoff, Jeffrey

    2010-11-01

    We studied the lapping of the domestic cat (Felis catus) by combining high-speed photography with a laboratory model of lapping. We found that Felis catus laps by a subtle mechanism based on water adhesion to the dorsal side of the tongue and the creation of a liquid column, exploiting inertia to defeat gravity and pull liquid into the mouth. The competition between inertia and gravity controls the pinch-off time of the column, determining the optimal lapping frequency, f. Felis catus was found to operate near the optimum and theoretical analysis yielded a scaling, f ˜M-1/6, of lapping frequency with animal mass, M. This prediction was verified by measuring lapping frequency across felids, from ocelots to lions, suggesting that the lapping mechanism is conserved among felines.

  15. Dicty_cDB: FC-AO13 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available dgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2191-29, mRNA sequence. ...76 4e-10 1 BM058578 |BM058578.1 2193-94 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides...bule subtracted cDNA library Ctenocephalides felis cDNA clone 2154-85, mRNA seque...privation. 68 9e-08 1 BM059287 |BM059287.1 2241-52 hindgut and Malpighian tubule cDNA library Ctenocephalides

  16. Dicty_cDB: CHQ652 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available t and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2193-94, mRNA sequence. 90 ...1e-13 1 BM058379 |BM058379.1 2191-29 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides f...d cDNA library Ctenocephalides felis cDNA clone 2154-85, mRNA sequence. 92 3e-14 1 DR447725 |DR447725.1 AR10.... 66 4e-18 3 BM056853 |BM056853.1 2154-85 hindgut and Malpighian tubule subtracte

  17. Malaria in the colombian regions of Uraba and Bajo Cauca, province of Antioquia: an overwiew to interpret the antimalarial therapeutic failure La malaria en Colombia, Antioquia y las zonas de Urabá y Bajo Cauca: panorama para interpretar la falla terapéutica antimalárica. Parte 2

    Directory of Open Access Journals (Sweden)

    Jaime Carmona Fonseca

    2004-01-01

    Full Text Available Problem: in the past 5 years we have studied the treatment response to antimalarials and their combinations in the Antioquia region (Turbo, Zaragoza and El Bagre municipalities. The interpretation and better understanding of the treatment outcome (adequate clinical response, early treatment failure or late treatment failure require further information since this response depends on host’s and parasite’s factors, independently of the characteristics of the antimalarial administered. Objectives: to define an adequate reference frame which allows for interpretation of results obtained during antimalarials treatment assessment studies. This would include demography, epidemiology, climatic and social-economic information about Colombia, Antioquia, the regions of Uraba, Bajo Cauca and the municipalities of Turbo and El Bagre. Methodology: research of secondary information (publications reports and internet resources, and some primary information (taken from Sistema de Vigilancia Epidemiológica de Antioquia (Sivigila of Dirección Seccional de Salud de Antioquia DSSA. Data about treatment response were primary information (produced by Grupo Malaria, Universidad de Antioquia, or obtained from other authors. With the gathered data, tables and graphs were produced and calculations were carried out using other variables. Results: the frequency of malaria is presented for Colombia during the period 1960-2002 and the results of the malaria eradication and control programs, and their failure, are described. Similarly, the frequency of malaria in Antioquia during 1959-2003 is discussed and presented in terms of incidence rates and parasite annual indexes (PAI. Non adjusted and adjusted rates and PAI were obtained, therefore adjusted rates are presented by exposed population, which is less of 20%. Adjusted results are 5-6 fold higher that non-adjusted values in Antioquia. However this is not true for the Uraba and Bajo Cauca regions, where 100% of the

  18. Numerical research on SASE FEL using a multi-frequency SDE code

    CERN Document Server

    Goto, M; Mima, K; Imasaki, K; Tsunawaki, Y; Yamanaka, C

    2000-01-01

    A multi-frequency 2D SASE FEL simulation code has been developed using the Source Dependent Expansion (SDE) method. With this code we calculated the evolution of the radiation amplitude and the spectrum of a SASE FEL using the beam parameters of FELI and the micro-wiggler developed by ILT/ILE/OSU. The effect of beam energy spread is also clarified.

  19. Rickettsia infection in five areas of the state of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Maurício C Horta

    2007-11-01

    Full Text Available This study investigated rickettsial infection in animals, humans, ticks, and fleas collected in five areas of the state of São Paulo. Eight flea species (Adoratopsylla antiquorum antiquorum, Ctenocephalides felis felis, Polygenis atopus, Polygenis rimatus, Polygenis roberti roberti, Polygenis tripus, Rhopalopsyllus lugubris, and Rhopalopsyllus lutzi lutzi, and five tick species (Amblyomma aureolatum, Amblyomma cajennense, Amblyomma dubitatum, Ixodes loricatus, and Rhipicephalus sanguineus were collected from dogs, cats, and opossums. Rickettsia felis was the only rickettsia found infecting fleas, whereas Rickettsia bellii was the only agent infecting ticks, but no animal or human blood was shown to contain rickettsial DNA. Testing animal and human sera by indirect immunofluorescence assay against four rickettsia antigens (R. rickettsii, R. parkeri, R. felis, and R. bellii, some opossum, dog, horse, and human sera reacted to R. rickettsii with titers at least four-fold higher than to the other three rickettsial antigens. These sera were considered to have a predominant antibody response to R. rickettsii. Using the same criteria, opossum, dog, and horse sera showed predominant antibody response to R. parkeri or a very closely related genotype. Our serological results suggest that both R. rickettsii and R. parkeri infected animals and/or humans in the studied areas.

  20. EST Table: FS933546 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS933546 E_FL_fwgP_37G09_F_0 10/09/28 41 %/186 aa gb|AAD21841.1| trypsin-like serine protease [Ctenocephalid...es felis] 10/09/13 low homology 10/08/29 n.h 10/09/10 37 %/190 aa AGAP004318-PA Pro

  1. EST Table: FY745967 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY745967 E_FL_famL_28K09_F_0 11/11/04 46 %/171 aa gb|AAD21841.1| trypsin-like serine protease [Ctenocephalid...es felis] 11/11/04 low homology 11/11/04 n.h 11/11/04 38 %/173 aa AGAP004318-PA Pro

  2. HISTOPATHOLOGICAL EVIDENCE OF REGENERATION FOLLOWING HEPATOTOXIC EFFECTS OF THE CYANOTOXIN MICROCYSTIN-LR IN THE HARDHEAD CATFISH AND GULF KILLIFISH.

    Science.gov (United States)

    The cyanobacterial toxin, microcystin-LR (MC-LR), causes liver damage in several freshwater fish species. The damage appears to be acute and irreversible. In the present study, two estuarine species, hardhead catfish, Arius felis, and gulf killifish, Fundulus grandis, were inject...

  3. Teaching A-level Genetics: The Coat Colours of the Domestic Cat.

    Science.gov (United States)

    Talbot, D. J.; Talbot, C. D.

    1992-01-01

    The authors provide an introduction to the inheritance of coat colors in cats and suggest strategies designed to integrate the domestic cat (Felis domesticus or catus) into the teaching of genetics. Provides examples to illustrate dominance, recessiveness, epistasis, multiple allelism, environmental effect of phenotype, incomplete dominance,…

  4. Will a hiding box provide stress reduction for shelter cats?

    NARCIS (Netherlands)

    Vinke, Claudia; Godijn, L.M.; van der Leij, Ruth

    2014-01-01

    Domestic cats (Felis sylvestris catus) can experience serious stress in shelters. Stressful experiences can have a major impact on the cats’ welfare and may cause higher incidences of infectious diseases in the shelters due to raised cortisol levels causing immuno deficiency.Though several studies s

  5. Assessment of PCR-DGGE for the identification of diverse Helicobacter species, and application to faecal samples from zoo animals to determine Helicobacter prevalence

    DEFF Research Database (Denmark)

    Abu Al-Soud, W.; Bennedsen, M.; On, Stephen L.W.;

    2003-01-01

    bilis and Helicobacter hepaticus in a Nile crocodile, Helicobacter cinaedi in a baboon and a red panda, and Helicobacter felis in a wolf and a Taiwan beauty snake. All of these PCR products (similar to400 bp) showed 100 % sequence similarity to 16S rDNA sequences of the mentioned species. These results...

  6. Dicty_cDB: SFH636 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ra acutorostrata CYP2C78... 60 3e-08 AB109547_1( AB109547 |pid:none) Balaenoptera a...98058 |pid:none) Felis catus CYP2E2 variant 1 mRNA ... 60 2e-08 AB290008_1( AB290008 |pid:none) Balaenopte

  7. A network of net-workers: report of the Euresco conference on 'Bacterial Neural Networks' held at San Feliu (Spain) from 8 to 14 May 2004

    NARCIS (Netherlands)

    K.J. Hellingwerf

    2004-01-01

    In May 2004, over 100 bacteriologists from 19 different countries discussed recent progress in identification and understanding of individual signal transfer mechanisms in bacteria and in the mutual interactions between these systems to form a functional living cell. The meeting was held in San Feli

  8. Hepatozoonosis in cats : ABCD guidelines on prevention and management

    NARCIS (Netherlands)

    Lloret, Albert; Addie, Diane D; Boucraut-Baralon, Corine; Egberink, Herman; Frymus, Tadeusz; Gruffydd-Jones, Tim; Hartmann, Katrin; Horzinek, Marian C; Hosie, Margaret J; Lutz, Hans; Marsilio, Fulvio; Pennisi, Maria Grazia; Radford, Alan D; Thiry, Etienne; Truyen, Uwe; Möstl, Karin

    2015-01-01

    OVERVIEW: Hepatozoonosis of domestic cats has been reported in several countries, mainly as a subclinical infection. DISEASE AGENT: Infection has been described mostly in areas where canine infection is present and, in recent years, Hepatozoon felis has been identified as a distinct species by molec

  9. Serological response of cats to experimental Besnoitia darlingi and Besnoitia neotomofelis infections and prevalence of antibodies to these parasites in cats from Virginia and Pennsylvania

    Science.gov (United States)

    Besnoitia darlingi and B. neotomofelis are tissue cyst-forming apicomplexan parasite that use domestic cats (Felis domesticus) as definitive hosts and opossums (Didelphis virginiana) and southern planes woodrats (Neotoma micropus) as intermediate hosts, respectively. Nothing is known about the preva...

  10. Real-Time Detection and Identification of Chlamydophila Species in Veterinary Specimens by Using SYBR Green-Based PCR Assays

    DEFF Research Database (Denmark)

    Nordentoft, Steen; Kabell, Susanne; Pedersen, Karl

    2011-01-01

    Chlamydiaceae and differentiate the most prevalent veterinary Chlamydophila species: Cp. psittaci, Cp. abortus, Cp. felis, and Cp. caviae. By adding bovine serum albumin to the master mixes, target DNA could be detected directly in crude lysates of enzymatically digested conjunctival or pharyngeal swabs or...

  11. Identification and estimation of size from the beaks of 18 species of cephalopods from the Pacific Ocean

    OpenAIRE

    Wolff, Gary A.

    1984-01-01

    A method of identifying the beaks and estimating body weight and mantle length of 18 species of cephalopods from the Pacific Ocean is presented. Twenty specimens were selected from each of the following cephalopod species: Symplectoteuthis oualaniensis, Dosidicus gigas, Ommastrephes bartramii, S. luminosa, Todarodes pacificus, Nototodarus hawaiiensis, Ornithoteuthis volalilis, Hyaloteuthis pelagica, Onychoteuthis banksii, Pterygioteuthis giardi, Abraliopsis affinis, A. felis, Liocranchia r...

  12. Estudios sobre Educacion, 2002 (Studies on Education, 2002).

    Science.gov (United States)

    Estudios sobre Educacion, 2002

    2002-01-01

    This scholarly journal deals with the study of education. Articles in this second issue are: "La Vertebracion Academica de la Formacion Inicial del Profesorado" (The Academic Backbone of the Initial Formation of Teachers) (Jose M. Esteve); "Deficiencia Visual en el Nino" (Visual Deficiency in the Young Child) (Feli Peralta; Juan Narbona); "The…

  13. IN SITU IMMUNE RESPONSE EVALUATION VIA IMMUNOHISTOCHEMISTRY IN SKIN BIOPSIES FROM PATIENTS AFFECTED BY AUTOIMMUNE BLISTERING DISEASES

    Directory of Open Access Journals (Sweden)

    Ana Maria Abreu Velez

    2013-11-01

    Full Text Available Introduction: The in situ immune response in skin biopsies from patients affected by autoimmune skin blistering diseases (ABD is not well characterized. Aim: Our investigation attempts to immunophenotype cells in lesional skin in several ABD, utilizing immunohistochemistry (ICH. Methods: We tested by IHC for CD4, CD8, CD19, CD20, CD45, CD56/NCAM, PAX-5, granzyme B, myeloperoxidase, neutrophil elastase, LAT and ZAP-70 in patients affected by ABD. We tested 30 patients with endemic pemphigus foliaceus (EPF, 15 controls from the EPF endemic area, and 15 biopsies from healthy controls from the USA. We also tested archival biopsies from patients with selected ABD, including 30 patients with bullous pemphigoid, 20 with pemphigus vulgaris, 8 with pemphigus foliaceus and 14 with dermatitis herpetiformis. Results: We found a predominantly CD8 positive/CD45 positive T cell infiltrate in all ABD. Our skin biopsies demonstrated consistently positive staining for myeloperoxidase, but negative staining for neutrophil elastase. Most ABD biopsies displayed negative staining for CD4 and B cell markers; natural killer cell markers were also rarely seen. ZAP-70 and LAT were frequently detected. In El Bagre-EPF, a significant fragmentation of T cells in lesional skin was noted, as well as autoreactivity to lymph nodes. Conclusions: The documented T cell and myeloperoxidase staining are indicative of the role of T lymphocytes and neutrophils in lesional biopsies in patients with ABD, in addition to previously documented deposition of B cells, immunoglobulins and complement in situ. In El Bagre-EPF, T cells could also target lymph nodes; however, further studies are needed to confirm this possibility.

  14. Malaria, desnutrición y parasitosis intestinal en los niños colombianos: interrelaciones interrrelations between malaria, malnutrition and intestinal parasitism in colombian children

    Directory of Open Access Journals (Sweden)

    Jaime Carmona Fonseca

    2004-09-01

    Full Text Available Este trabajo resume datos colombianos y los hallazgos del Grupo Malaria (Universidad de Antioquia sobre las relaciones entre malaria, desnutrición y funcionamiento inmunitario, trabajos realizados en niños (4-11 años de las poblaciones antioqueñas de Turbo, El Bagre y Zaragoza. Se hace énfasis en las relaciones malaria, parásitos intestinales y desnutrición (desnutrición crónica, hipovitaminosis A, las cuales se exploran a través de su articulación en el sistema inmunitario. Se formulan recomendaciones para la aplicación clínica (individual y epidemiológica (colectiva con respecto a la formulación de suplemento de vitamina A y antihelmínticos de amplio espectro. En Turbo y en El Bagre y Zaragoza: 1 la frecuencia de malaria registra índices parasitarios anuales de 39 (Turbo y 156 casos por cada mil sujetos expuestos (El Bagre-Zaragoza en 1996-2000; 2 el riesgo de desnutrición crónica (índice talla/ edad es de 63% en los niños de 3-11 años; 3 se encontró anemia en el 26% de los palúdicos y 17% de los no palúdicos; 4 el 65% de los niños con malaria y el 35% de aquellos sin malaria mostraron valores de retinol bajos (<0,3 µg/mL; 5 se hallaron niveles anormalmente bajos de apoproteína A-1, tanto en los niños sin malaria como, sobre todo, en los que sí la tenían; 6 el 96% de los niños (4-9 años con malaria mostraron títulos de interleuquina 10 (IL10 muy altos con relación a lo normal y estadísticamente superiores a los exhibidos por los niños sin malaria; 7 los títulos de IgE total y específica anti-Plasmodium estuvieron anormalmente altos en los niños de ambos municipios e igual sucedió con los niveles de FNT-; 8 en estudiantes y docentes de enfermería, aparentemente sanos, con edades de 18-44 años, hallamos parásitos intestinales en el 97% y parásitos intestinales patógenos en el 42%. En menores de 5 años de Turbo hay parásitos intestinales patógenos en un 30-35% de los niños, con predominio de G

  15. Ectoparasitos de cães e gatos da cidade de Manaus, Amazonas, Brasil Ectoparasites on cats and dogs from Manaus, Amazonas State, Brazil

    Directory of Open Access Journals (Sweden)

    Marcelo Cutrim Moreira de Castro

    2006-12-01

    Full Text Available São apresentados resultados da coleta de ectoparasitos em cães e gatos entre agosto de 2001 e maio de 2002 em diferentes bairros da cidade Manaus. No cão foram encontrados: Ctenocephalides f. felis (Bouché, 1835 (Siphonaptera, Pulicidae, Heterodoxus spiniger (Enderlein, 1909(Phthiraptera, Boopidae, Trichodectes canis (De Geer, 1778 (Phthiraptera, Trichodectidae e Rhipicephalus sanguineus (Latreille,1806 (Acari, Ixodidae. No gato foi coletado C. f. felis. A prevalência de ectoparasitos foi de 80,8% para cães e 72,7% para gatos. Para a pulga C. f. felis foi de 28,7% para cães e 72,7% para gatos. Para o piolho H. spiniger foi de 12,3% para cães. Para o piolho T. canis foi de 0,1% para cães e para o carrapato R. sanguineus foi de 63% para cães. A média de infestaçãode pulga foi de 1,26 para cães e 1,27 para gatos. A proporção sexual fêmea/macho foi de 1,96:1 no cão e de 3,66:1 no gato. A pulga C. canis (Curtis, 1826, registrada em 1922, não foi coletada.Ectoparasites from different neighborhood of Manaus were collected from august 2001 to May 2002. On dogs it was found: Ctenocephalides f. felis (Bouché, 1835 (Siphonaptera, Pulicidae, Heterodoxus spiniger (Enderlein, 1909(Phthiraptera, Boopidae, Trichodetes canis (De Geer, 1778 (Phthiraptera, Trichodectidae and Rhipicephalus sanguineus (Latreille,1806 (Acari, Ixodidae. On cats: C. f. felis. The prevalence of ectoparasites was 80.8% to dogs and 72.7% to cats. For the flea C. f. felis was 28.7% to dogs and 72.7% to cats. For the lice H. spiniger was 12.3% for dogs. For the lice T. canis was 0.1% for dogs and for the tick R. sanguineus was 63% for dogs. The infestation index for fleas was 1.26 to dogs and 1.27 to cats. The sexual ratio obtained was 1.96:1 to dogs and 3.66:1 to cats. The flea C. canis (Curtis, 1826 registered in 1922 was not found.

  16. Avaliação de alguns parâmetros ecocardiográficos do gato-do-mato (Leopardus tigrinus, mantido em cativeiro e submetido à anestesia com xilazina e quetamina Evaluation of some echocardiographic parameters of Oncilla (L. tigrinus, kept in captivity and submitted to anesthesia with xilazine and ketamine

    Directory of Open Access Journals (Sweden)

    P.S.L. Carvalho

    2007-06-01

    Full Text Available Avaliaram-se alguns parâmetros ecocardiográficos em modos B, M e Doppler de 27 gatos-do-mato, Leopardus tigrinus, pequeno felídeo selvagem, mantidos em cativeiro e submetidos à anestesia com 1 a 2mg/kg de xilazina e 10mg/kg de quetamina. Observaram-se alterações dos parâmetros cardiovasculares quando os resultados foram comparados aos do gato doméstico (Felis catus não anestesiado.Some echocardiographic parameters in B, M-mode and Doppler of 27 Oncillas, Leopardus tigrinus, a wild little feline, kept in captivity and submitted to anesthesia with 1 to 2mg/kg of xilazine and 10mg/kg of ketamine, had been evaluated. Changes of the cardiovascular parameters were observed when the results were compared to non anesthetized domestic cat (Felis catus.

  17. Faecal helminth egg and oocyst counts of a small population of African lions (Panthera leo in the southwestern Kalahari, Namibia : research communication

    Directory of Open Access Journals (Sweden)

    Y. Smith

    2006-09-01

    Full Text Available An endoparasite survey of a small pride of African lions (Panthera leo was conducted at Intu Afrika Kalahari Game Reserve, southwestern Namibia, during winter and summer of 2003 and 2004, respectively. Overall, 23 fresh lion scats were collected opportunistically during fieldwork trials. A flotation technique was employed for the diagnosis of parasites. Three nematodes, Ancylostoma braziliense, Gnathostoma spinigerum and Uncinaria stenocephala and two coccidians, Toxoplasma gondii and Isospora felis were recorded. By using the McMaster method for quantification, a maximum number of 14 866 oocysts per gram of faeces was obtained for I. felis during winter 2003. Endoparasite taxa carried by the different individuals in the pride were found to be related to their levels of association. Rates of infection were relatively low as a result of the habitat, semi-captive conditions and earlier sporadic deworming.

  18. Parasites of domestic and wild canids in the region of Serra do Cipó National Park, Brazil Parasitos de canídeos domésticos e silvestres na região do Parque Nacional da Serra do Cipó - Brasil

    Directory of Open Access Journals (Sweden)

    Juliana Lúcia Costa Santos

    2012-09-01

    Full Text Available Over recent decades, diseases have been shown to be important causes of extinctions among wild species. Greater emphasis has been given to diseases transmitted by domestic animals, which have been increasing in numbers in natural areas, along with human populations. This study had the aim of investigating the presence of intestinal helminths in wild canids (maned wolf, Chrysocyon brachyurus, and crab-eating fox, Cerdocyon thous in the Serra do Cipó National Park (43-44º W and 19-20º S and endo and ectoparasites of domestic dogs in the Morro da Pedreira Environmental Protection Area (an area surrounding the National Park. The Serra do Cipó is located in the state of Minas Gerais, Brazil. Among the enteroparasites found in domestic and wild canids, the following taxons were identified: Ancylostomidae, Trichuridae, Toxocara sp., Spirocerca sp., Physaloptera sp., Strongyloides sp., Cestoda, Dipylidium caninum, Diphyllobothriidae, Hymenolepidae, Anoplocephalidae, Trematoda, Acanthocephala and Isospora sp. Domestic dogs were positive for leishmaniasis and Babesia canis in serological tests. Among the ectoparasites, Rhipicephalus sanguineus, Amblyomma cajennense and Ctenocephalides felis felis were observed in domestic dogs. Variations in the chaetotaxy of the meta-episternum and posterior tibia were observed in some specimens of C. felis felis.Nas últimas décadas, as doenças têm sido apontadas como importantes causas de extinção de espécies silvestres. Maior ênfase tem sido dada às doenças transmitidas por animais domésticos que crescem em número, bem como as populações humanas, em áreas naturais. O presente estudo objetivou verificar a presença de helmintos intestinais de canídeos silvestres (lobo-guará - Chrysocyon brachyurus e cachorro-do-mato - Cerdocyon thous do Parque Nacional da Serra do Cipó (43-44º W e 19-20º S e endo e ectoparasitos de cães domésticos da Área de Proteção Ambiental Morro da Pedreira (entorno do

  19. Insecticide Resistance in Fleas.

    Science.gov (United States)

    Rust, Michael K

    2016-01-01

    Fleas are the major ectoparasite of cats, dogs, and rodents worldwide and potential vectors of animal diseases. In the past two decades the majority of new control treatments have been either topically applied or orally administered to the host. Most reports concerning the development of insecticide resistance deal with the cat flea, Ctenocephalides felis felis. Historically, insecticide resistance has developed to many of the insecticides used to control fleas in the environment including carbamates, organophosphates, and pyrethroids. Product failures have been reported with some of the new topical treatments, but actual resistance has not yet been demonstrated. Failures have often been attributed to operational factors such as failure to adequately treat the pet and follow label directions. With the addition of so many new chemistries additional monitoring of flea populations is needed. PMID:26999217

  20. The Near Eastern origin of cat domestication.

    Science.gov (United States)

    Driscoll, Carlos A; Menotti-Raymond, Marilyn; Roca, Alfred L; Hupe, Karsten; Johnson, Warren E; Geffen, Eli; Harley, Eric H; Delibes, Miguel; Pontier, Dominique; Kitchener, Andrew C; Yamaguchi, Nobuyuki; O'brien, Stephen J; Macdonald, David W

    2007-07-27

    The world's domestic cats carry patterns of sequence variation in their genome that reflect a history of domestication and breed development. A genetic assessment of 979 domestic cats and their wild progenitors-Felis silvestris silvestris (European wildcat), F. s. lybica (Near Eastern wildcat), F. s. ornata (central Asian wildcat), F. s. cafra (southern African wildcat), and F. s. bieti (Chinese desert cat)-indicated that each wild group represents a distinctive subspecies of Felis silvestris. Further analysis revealed that cats were domesticated in the Near East, probably coincident with agricultural village development in the Fertile Crescent. Domestic cats derive from at least five founders from across this region, whose descendants were transported across the world by human assistance.

  1. Mucosal Immunization with Helicobacter, CpG DNA, and Cholera Toxin Is Protective

    OpenAIRE

    Jiang, Weiwen; Baker, Henry J.; Smith, Bruce F.

    2003-01-01

    The mucosal delivery of antigens requires an effective adjuvant to induce mucosal immunity. Current mucosal adjuvants include cholera toxin (CT) and Escherichia coli heat-labile toxin. Unmethylated CpG immunostimulatory oligodeoxynucleotides (ODNs) have been proposed as novel mucosal adjuvants. In this study, mice were immunized with sonicated Helicobacter felis with CT and/or CpG ODN adjuvants. All groups receiving either adjuvant singly or in combination developed increased serum anti-H. fe...

  2. EST Table: BJ985655 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available BJ985655 E_FL_mxg-_19A07_F_0 10/09/28 35 %/132 aa gb|AAD21841.1| trypsin-like serine protease [Ctenocephalid...es felis] 10/08/28 n.h 10/08/28 n.h 10/09/10 n.h 10/09/10 n.h 10/09/10 n.h FS930815 mxg- ...

  3. Dicty_cDB: CHG233 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available sequence. 66 2e-18 3 BM056853 |BM056853.1 2154-85 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides...pighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2193-94, ...mRNA sequence. 90 9e-14 1 BM058379 |BM058379.1 2191-29 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides

  4. Dicty_cDB: SHG302 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ;, mRNA sequence. 66 3e-18 3 BM056853 |BM056853.1 2154-85 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides...ighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2193-94, m...RNA sequence. 82 3e-11 1 BM058379 |BM058379.1 2191-29 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides

  5. EST Table: FS741218 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available ike protein 1 [Ctenocephalides felis] 10/09/07 low homology 10/08/28 n.h 10/09/10 low homology 10/09/10 low ...FS741218 E_FL_bmmt_19G24_R_0 11/12/09 n.h 10/09/28 30 %/275 aa gb|AAQ88394.1| SV2-l

  6. Dicty_cDB: SHA868 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available sequence. 66 3e-18 3 BM056853 |BM056853.1 2154-85 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides...bule subtracted cDNA library Ctenocephalides felis cDNA clone 2193-94, mRNA seque...nce. 90 1e-13 1 BM058379 |BM058379.1 2191-29 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides

  7. Comparative anatomy of the cardiac foramen ovale in cats (Felidae), dogs (Canidae), bears (Ursidae) and hyaenas (Hyaenidae).

    OpenAIRE

    Macdonald, A A; Johnstone, M.

    1995-01-01

    The structure of the foramen ovale from 16 species representing 4 carnivore families, the Felidae, Canidae, Ursidae and Hyaenidae, was studied using the scanning electron microscope. The Felidae were represented by 9 domestic cat fetuses (Felis catus), 2 snow leopard neonates (Uncia uncia), an ocelot neonate (Leopardus pardalis), 2 lion neonates (Panthera leo), a panther neonate (Panthera pardus) and 3 tigers (Neofelis tigris), comprising 2 fetuses and a neonate. The Canidae were represented ...

  8. Mapping trends of large and medium size carnivores of conservation interest in Romania

    OpenAIRE

    Mihai Cristian Adamescu; Constantin Cazacu; Ovidiu Ionescu; Georgeta Ionescu; Ramon Jurj; Marius Popa; Roxana Cazacu; Ancuta Cotovelea

    2014-01-01

    We analysed yearly estimates of population size data during 2001-2012 for five carnivores species of conservation interest (Ursus arctos, Canis lupus, Lynx lynx, Felis silvestris and Canis aureus). Population size estimations were done by the game management authorities and integrated by the competent authorities on the Ministry of Environment and Climate Change. Trends in data were detected using non-parametric Mann-Kendall test. This test was chosen considering the short length of data seri...

  9. AcEST: BP918525 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 8 0.028 sp|P02836|HMEN_DROME Segmentation polarity homeobox protein engr... 36 0.10 sp|Q7XC27|CNBL1_ORYSJ Ca...ing protein 2 OS=Felis silve... 32 2.0 sp|P09145|HMEN_DROVI Segmentation polarity homeobox protein engr... 3...SSNTTPTSTTF 287 Query: 188 FGSVPSAA 165 + PS A Sbjct: 288 TTTSPSTA 295 >sp|P02836|HMEN_DROME Segmentation

  10. Genetic and phylogenetic divergence of feline immunodeficiency virus in the puma (Puma concolor).

    OpenAIRE

    Carpenter, M.A.; Brown, E. W.; Culver, M; Johnson, W E; Pecon-Slattery, J; Brousset, D; O'Brien, S J

    1996-01-01

    Feline immunodeficiency virus (FIV) is a lentivirus which causes an AIDS-like disease in domestic cats (Felis catus). A number of other felid species, including the puma (Puma concolor), carry a virus closely related to domestic cat FIV. Serological testing revealed the presence of antibodies to FIV in 22% of 434 samples from throughout the geographic range of the puma. FIV-Pco pol gene sequences isolated from pumas revealed extensive sequence diversity, greater than has been documented in th...

  11. Survey of Helicobacter infection in domestic and feral cats in Korea

    OpenAIRE

    Ghil, Heh-Myung; Yoo, Jong-Hyeon; Jung, Woo-Sung; CHUNG, Tae-Ho; Youn, Hwa-Young; Hwang, Cheol-Yong

    2009-01-01

    Discovery of Helicobacter (H.) pylori has led to a fundamental change in our understanding of gastric diseases in humans. Previous studies have found various Helicobacter spp. in dogs and cats, and pets have been questioned as a zoonotic carrier. The present study surveyed the Helicobacter infections and investigated the presence of H. felis and H. pylori infections in domestic and feral cats in Korea. Sixty-four domestic cats and 101 feral cats were selected from an animal shelter. Saliva an...

  12. Stable individual differences in separation calls during early development in cats and mice

    OpenAIRE

    Hudson, Robyn; Rangassamy, Marylin; Saldaña, Amor; Bánszegi, Oxána; Rödel, Heiko G

    2015-01-01

    Background The development of ethologically meaningful test paradigms in young animals is an essential step in the study of the ontogeny of animal personality. Here we explore the possibility to integrate offspring separation (distress) calls into the study of consistent individual differences in behaviour in two species of mammals, the domestic cat (Felis silvestris catus) and the mound-building mouse (Mus spicilegus). Such vocal responses in young mammals are a potentially useful test optio...

  13. Serosurvey of Smooth Brucella, Leptospira spp. and Toxoplasma gondii in Free-Ranging Jaguars (Panthera onca) and Domestic Animals from Brazil

    OpenAIRE

    Mariana Malzoni Furtado; Solange Maria Gennari; Cassia Yumi Ikuta; Anah Tereza de Almeida Jácomo; Zenaide Maria de Morais; Hilda Fátima de Jesus Pena; Grasiela Edith de Oliveira Porfírio; Leandro Silveira; Rahel Sollmann; Gisele Oliveira de Souza; Natália Mundim Tôrres; José Soares Ferreira Neto

    2015-01-01

    This study investigated the exposure of jaguar populations and domestic animals to smooth Brucella, Leptospira spp. and Toxoplasma gondii in the Cerrado, Pantanal and Amazon biomes of Brazil. Between February 2000 and January 2010, serum samples from 31 jaguars (Panthera onca), 1,245 cattle (Bos taurus), 168 domestic dogs (Canis lupus familiaris) and 29 domestic cats (Felis catus) were collected and analysed by rose bengal test for smooth Brucella, microscopic agglutination test for Leptospir...

  14. Paraziti koček

    OpenAIRE

    Poláková, Zuzana

    2012-01-01

    SUMMARY Cat parasites This thesis is based on scientific literature and discusses the most important representatives of cat parasites. I selected three representatives of the protozoan parasites in cats: Toxoplasma gondii, Giardia felis and Cryptosporidium parvum. They have large zoonotic potential. From the group of trematodes, I chose the lung fluke (Paragonimus westermani). From the species of cestoda, I picked the cucumber tapeworm (Dipylidium caninum), the cat tapeworm (Taenia taen...

  15. Invertebrate vectors, parasites, and rickettsial agents in Guam

    Directory of Open Access Journals (Sweden)

    Johnson, J.

    2012-12-01

    Full Text Available We conducted a 3-week field study of ectoparasites of humans and domestic animals throughout Guam. Thirteen species of ectoparasitic arthropods were collected. Ectoparasites of medical or veterinary significance included the ticks Rhipicephalus sanguineus and Rhipicephalus microplus, fleas Ctenocephalides felis and Xenopsylla cheopsis, and the head louse Pediculus humanus capitis. Polymerase chain reaction based screening for rickettsial and protozoan pathogens detected pathogens in eight arthropods. These included Anaplasma platys, Coxiella burnetii, Babesia canis vogeli, and Hepatozoon canis.

  16. Competition and intraguild predation among three sympatric carnivores

    OpenAIRE

    Fedriani, José M.; Fuller, Todd K.; Sauvajot, Raymond M.; York, Eric C.

    2000-01-01

    We examined the relative roles of dominance in agonistic interactions and energetic constraints related to body size in determining local abundances of coyotes (Canis latrans, 8-20 kg), gray foxes (Urocyon cinereoargenteus, 3-5 kg) and bobcats (Felis rufus, 5-15 kg) at three study sites (hereafter referred to as NP, CP, and SP) in the Santa Monica Mountains of California. We hypothesized that the largest and behaviorally dominant species, the coyote, would exploit a wider range of resources (...

  17. Exposure to positively- and negatively-charged plasma cluster ions impairs IgE-binding capacity of indoor cat and fungal allergens

    OpenAIRE

    NISHIKAWA, Kazuo; Fujimura, Takashi; Ota, Yasuhiro; Abe, Takuya; ElRamlawy, Kareem Gamal; Nakano, Miyako; Takado, Tomoaki; Uenishi, Akira; Kawazoe, Hidechika; Sekoguchi, Yoshinori; Tanaka, Akihiko; Ono, Kazuhisa; Kawamoto, Seiji

    2016-01-01

    Background Environmental control to reduce the amount of allergens in a living place is thought to be important to avoid sensitization to airborne allergens. However, efficacy of environmental control on inactivation of airborne allergens is not fully investigated. We have previously reported that positively- and negatively-charged plasma cluster ions (PC-ions) reduce the IgE-binding capacity of crude allergens from Japanese cedar pollen as important seasonal airborne allergens. Cat (Felis do...

  18. The Role of Antigenic Drive and Tumor-Infiltrating Accessory Cells in the Pathogenesis of Helicobacter-Induced Mucosa-Associated Lymphoid Tissue Lymphoma

    OpenAIRE

    Mueller, Anne; O’Rourke, Jani; Chu, Pauline; Chu, Amanda; Michael F. Dixon; Bouley, Donna M.; Lee, Adrian; Falkow, Stanley

    2005-01-01

    Gastric B-cell lymphoma of mucosa-associated lymphoid tissue type is closely linked to chronic Helicobacter pylori infection. Most clinical and histopathological features of the tumor can be reproduced by prolonged Helicobacter infection of BALB/c mice. In this study, we have addressed the role of antigenic stimulation in the pathogenesis of the lymphoma by experimental infection with Helicobacter felis, followed by antibiotic eradication therapy and subsequent re-infection. Antimicrobial the...

  19. Parenteral Adjuvant Activities of Escherichia coli Heat-Labile Toxin and Its B Subunit for Immunization of Mice against Gastric Helicobacter pylori Infection

    OpenAIRE

    Weltzin, Richard; Guy, Bruno; Thomas, William D.; Giannasca, Paul J.; Monath, Thomas P.

    2000-01-01

    The heat-labile toxin (LT) of Escherichia coli is a potent mucosal adjuvant that has been used to induce protective immunity against Helicobacter felis and Helicobacter pylori infection in mice. We studied whether recombinant LT or its B subunit (LTB) has adjuvant activity in mice when delivered with H. pylori urease antigen via the parenteral route. Mice were immunized subcutaneously or intradermally with urease plus LT, recombinant LTB, or a combination of LT and LTB prior to intragastric c...

  20. Estimating free-roaming cat densities in urban areas: comparison of mark-resight and distance sampling

    OpenAIRE

    Schmidt, P. M.; Lopez, R. R.; Pierce, B. L.

    2007-01-01

    Obtaining reliable population estimates is imperative in managing wildlife populations, particularly when attempting to implement nuisance control measures. Free-roaming cats (Felis catus) impact wildlife worldwide through predation, competition and disease transmission. Ideally, measures of controlling free-roaming cat populations should be evaluated a priori, which requires obtaining population estimates for use in population control programs (e.g., euthanasia, trap/treat/neuter/release). W...

  1. Localization of Helicobacter spp. in the fundic mucosa of laboratory Beagle dogs: an ultrastructural study

    Directory of Open Access Journals (Sweden)

    Lanzoni Anna

    2011-03-01

    Full Text Available Abstract In dogs Helicobacter spp. are found in all gastric regions usually localized in the surface mucus, gastric glands and parietal cells. The aim of this study was to detail the distribution of Helicobacter spp. in the fundic mucosa of asymptomatic Beagle dogs and their intracellular localization within parietal cells, in order to evaluate species-specific pathogenetic effects on gastric cells. The presence of Helicobacter spp. was investigated by immunohistochemistry, TEM, and PCR in the fundic mucosa of six Beagle dogs. Helicobacter spp. were found in all dogs examined, and H. bizzozeronii and H. felis were identified by PCR and confirmed by TEM. In the lumen of the fundic glands, co-localization was common. H. bizzozeronii was present in larger numbers than H. felis in both intraluminal and intraparietal localization. The amounts of H. bizzozeronii were similar in superficial and basal portions of the glands. H. felis was predominantly localized in the superficial portions of gastric glands but almost absent from the base. Within parietal cells, most Helicobacter organisms were intracanalicular, but intact and degenerate Helicobacter organisms were also visualized free in the cytoplasm or in secondary lysosomes. No specific degenerative lesions were found in infected parietal cells. Helicobacter organisms were also observed within macrophages in the lamina propria. In conclusion, there is a differential distribution of H. bizzozeronii and H. felis in the fundic mucosa of Beagle dogs, and their intracellular localization in parietal cells and macrophages suggests novel pathogenic scenarios for the development of immune response and maintenance of chronic gastritis in dogs.

  2. A Molecular survey of Anaplasma spp., Rickettsia spp., Ehrlichia canis and Babesia microti in foxes and fleas from Sicily.

    Science.gov (United States)

    Torina, A; Blanda, V; Antoci, F; Scimeca, S; D'Agostino, R; Scariano, E; Piazza, A; Galluzzo, P; Giudice, E; Caracappa, S

    2013-11-01

    Fleas (Insecta: Siphonaptera) are obligate bloodsucking insects, which parasitize birds and mammals, and are distributed throughout the world. Several species have been implicated in pathogen transmission. This study aimed to monitor red foxes and the fleas isolated from them in the Palermo and Ragusa provinces of Sicily, Italy, as these organisms are potential reservoirs and vectors of pathogens. Thirteen foxes (Vulpes vulpes) and 110 fleas were analysed by polymerase chain reaction (PCR) to detect DNA of the pathogens Ehrlichia canis, Babesia microti, Rickettsia spp., Anaplasma phagocytophilum, Anaplasma platys, Anaplasma marginale and Anaplasma ovis. In the foxes, A. ovis was detected in only one animal, whereas the prevalence of the E. canis pathogen was 31%. B. microti and Rickettsia spp. were not detected. Of all of the collected fleas, 75 belonged to the species Xenopsylla cheopis, 32 belonged to Ctenocephalides canis, two belonged to Ctenocephalides felis and one belonged to Cediopsylla inaequalis. In the fleas, the following pathogens were found: A. ovis (prevalence 25%), A. marginale (1%), A. phagocytophilum (1%), Rickettsia felis (2%) and E. canis (3%). X. cheopis was the flea species most frequently infected with Anaplasma, in particular A. ovis (33%), A. marginale (1%) and A. phagocytophilum (1%). Both C. felis exemplars were positive for R. felis. E. canis was found in the lone C. inaequalis and also in 3% of the X. cheopis specimens. No fleas were positive for B. microti or A. platys. As foxes often live in proximity to domestic areas, they may constitute potential reservoirs for human and animal parasites. Further studies should be performed on fleas to determine their vectorial capacity.

  3. Journal of Parasitology

    OpenAIRE

    Hsu, V; Grant, D.C.; Dubey, J.P.; Zajac, A. M.; Lindsay, D S

    2010-01-01

    Sarcocystis neurone, is best known as the causative agent of equine protozoal myeloencephalitis of horses in the Americas. Domestic cats (Felis domesticus) were the first animals described as an intermediate host for S. neurone,. However, S. neurona-associated encephalitis has also been reported in naturally infected cats in the United States. Thus, cats can be implicated in the life cycle of S. neurona as natural intermediate hosts. The present study examined the seroprevalence of IgG antibo...

  4. Wild dogma: An examination of recent “evidence” for dingo regulation of invasive mesopredator release in Australia

    OpenAIRE

    Benjamin L. ALLEN, Richard M. ENGEMAN, Lee R. ALLEN

    2011-01-01

    There is growing interest in the role that apex predators play in shaping terrestrial ecosystems and maintaining trophic cascades. In line with the mesopredator release hypothesis, Australian dingoes (Canis lupus dingo and hybrids) are assumed by many to regulate the abundance of invasive mesopredators, such as red foxes Vulpes vulpes and feral cats Felis catus, thereby providing indirect benefits to various threatened vertebrates. Several recent papers have claimed to provide evidence for th...

  5. Dicty_cDB: VSI381 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 006861 |pid:none) Chlamydophila felis Fe/C-56 DNA,... 35 3.5 D38156_1( D38156 |pid:none) Equine rotavirus RN...8155 |pid:none) Equine rotavirus RNA 5 for NSP1, compl... 35 3.5 AP006861_626( AP...ides DSM... 35 3.5 CP000409_286( CP000409 |pid:none) Rickettsia canadensis str. McKie... 35 3.5 D38155_1( D3

  6. Protective immunity against Helicobacter is characterized by a unique transcriptional signature

    OpenAIRE

    Mueller, Anne; O'Rourke, Jani; Chu, Pauline; Kim, Charles C; Sutton, Philip; Lee, Adrian; Falkow, Stanley

    2003-01-01

    Immunization with a whole-cell sonicate vaccine of Helicobacter felis in conjunction with cholera toxin as a mucosal adjuvant induces long-term protective immunity in a majority of laboratory mice. We have combined gene expression profiling and immunohistochemical analysis on a set of immunized animals to better understand the mechanism of protection. The stomachs of protected animals exhibited a strikingly different transcriptional profile compared with those of nonprotected or control mice,...

  7. Temperature dependence of small polaron population decays in iron-doped lithium niobate by Monte Carlo simulations

    Science.gov (United States)

    Mhaouech, I.; Guilbert, L.

    2016-10-01

    The population decay of light-induced small polarons in iron-doped lithium niobate is simulated by a Monte-Carlo method on the basis of Holstein's theory. The model considers random walks of both bound polarons (NbLi4+) and free polarons (NbNb4+) ending to deep traps (FeLi3+). The thermokinetic interplay between polaron species is introduced by trapping and de-trapping rates at niobium antisites (NbLi). The decay of the NbLi4+ population proceeds by three possible channels: direct trapping at FeLi3+ sites, hopping on niobium antisites and hopping on Nb regular sites after conversion to the free state. Up to three regimes, each one reflecting the predominance of one of these processes, appear with different activation energies in the Arrhenius plots of the decay time. The influence of FeLi and NbLi concentrations on the transition temperatures is evidenced. For both polaron species, the length of the final hop (trapping length) is found much larger than the usual hopping length and decreases at rising temperature. This trap size effect is a natural consequence of Holstein's theory and may explain some unclear features of polaron-related light-induced phenomena, such as the temperature-dependent stretching exponent of light-induced absorption decays and the anomalous increase of the photoconductivity at high doping levels.

  8. Flea-borne rickettsioses in the north of Caldas province, Colombia.

    Science.gov (United States)

    Hidalgo, Marylin; Montoya, Viviana; Martínez, Alejandra; Mercado, Marcela; De la Ossa, Alberto; Vélez, Carolina; Estrada, Gloria; Pérez, Jorge E; Faccini-Martínez, Alvaro A; Labruna, Marcelo B; Valbuena, Gustavo

    2013-05-01

    Rickettsia typhi and R. felis are the etiological agents of murine typhus and flea-borne spotted fever, respectively. Both are emerging acute febrile zoonotic diseases for which fleas are vectors; they also have similar clinical characteristics and global distribution. In 2005, we identified the circulation of murine typhus in 6 towns within the mountainous coffee-growing area north of Caldas, Colombia. We now report the specific seroprevalence against R. typhi and R. felis, and associated risk factors in 7 towns of this province. The combined seroprevalence against the 2 flea-borne rickettsioses is the highest yet reported in the literature: 71.7% (17.8% for R. felis, 25.2% for R. typhi, and 28.7% for both). We also report a prospective analysis of 26 patients with a febrile illness compatible with rickettsioses, including murine typhus; 9 of these patients had a rickettsiosis. This supports our sero-epidemiological results and highlights the diagnostic complexity of febrile syndromes in this region. PMID:23473218

  9. Survey of Helicobacter infection in domestic and feral cats in Korea.

    Science.gov (United States)

    Ghil, Heh-Myung; Yoo, Jong-Hyeon; Jung, Woo-Sung; Chung, Tae-Ho; Youn, Hwa-Young; Hwang, Cheol-Yong

    2009-03-01

    Discovery of Helicobacter (H.) pylori has led to a fundamental change in our understanding of gastric diseases in humans. Previous studies have found various Helicobacter spp. in dogs and cats, and pets have been questioned as a zoonotic carrier. The present study surveyed the Helicobacter infections and investigated the presence of H. felis and H. pylori infections in domestic and feral cats in Korea. Sixty-four domestic cats and 101 feral cats were selected from an animal shelter. Saliva and feces were evaluated by Helicobacter genus-specific polymerase chain reaction (PCR). Genus-specific PCR positive samples were further evaluated for H. felis and H. pylori using specific primer pairs. Thirty-six of 64 (56.3%) samples from domestic cats and 92 of 101 (91.1%) samples from feral cats were PCR positive; the positive rate of feces samples was higher than that of saliva samples in both groups. H. felis and H. pylori species-specific PCR was uniformly negative. The prevalence of Helicobacter spp. in feral cats was approximately two-fold higher than that of domestic cats. The fecal-oral route may be more a common transmission route not only between cats but also in humans. PMID:19255526

  10. Isolation of Candidatus Rickettsia asemboensis from Ctenocephalides Fleas.

    Science.gov (United States)

    Luce-Fedrow, Alison; Maina, Alice N; Otiang, Elkanah; Ade, Fredrick; Omulo, Sylvia; Ogola, Eric; Ochieng, Linus; Njenga, M Kariuki; Richards, Allen L

    2015-04-01

    Candidatus Rickettsia asemboensis was identified molecularly in fleas collected in 2009 from Asembo, Kenya. Multilocus sequence typing using the 17-kD antigen gene, rrs, gltA, ompA, ompB, and sca4 demonstrated that Candidatus R. asemboensis is closely related to Rickettsia felis but distinct enough to be considered for separate species classification. Following this molecular characterization of Candidatus R. asemboensis, the in vitro cultivation of this bacterium was then performed. We used Ctenocephalides canis and Ctenocephalides felis fleas removed from dogs in Kenya to initiate the in vitro isolation of Candidatus R. asemboensis. Successful cultures were obtained using Drosophila melanogaster S2 and Aedes albopictus C6/36 cell lines. Cytological staining and quantitative real-time PCR (qPCR) assays were used to visualize/confirm the culture of the bacteria in both cell lines. Sequencing of fragments of the 17-kD antigen gene, gltA, and ompB genes confirmed the identity of our Candidatus R. asemboensis isolates. To date, we have passaged Candidatus R. asemboensis 12 times through S2 and C6/36 cells, and active and frozen cultures are currently being maintained. This is the first time that a R. felis-like organism has been grown and maintained in culture and is therefore the first time that one of them, Candidatus R. asemboensis, has been characterized beyond molecular typing.

  11. Rickettsia species in fleas collected from small mammals in Slovakia.

    Science.gov (United States)

    Špitalská, Eva; Boldiš, Vojtech; Mošanský, Ladislav; Sparagano, Olivier; Stanko, Michal

    2015-11-01

    Epidemiological and epizootiological studies of Rickettsia felis and other Rickettsia spp. are very important, because their natural cycle has not yet been established completely. In total, 315 fleas (Siphonaptera) of 11 species of Ceratophyllidae, Hystrichopsyllidae and Leptopsyllidae families were tested for the presence of Rickettsia species and Coxiella burnetii with conventional and specific quantitative real-time PCR assays. Fleas were collected from five rodent hosts (Myodes glareolus, Apodemus flavicollis, Apodemus agrarius, Microtus subterraneus, Microtus arvalis) and three shrew species (Sorex araneus, Neomys fodiens, Crocidura suaveolens) captured in Eastern and Southern Slovakia. Overall, Rickettsia spp. was found in 10.8% (34/315) of the tested fleas of Ctenophthalmus agyrtes, Ctenophthalmus solutus, Ctenophthalmus uncinatus and Nosopsyllus fasciatus species. Infected fleas were coming from A. flavicollis, A. agrarius, and M. glareolus captured in Eastern Slovakia. C. burnetii was not found in any fleas. R. felis, Rickettsia helvetica, unidentified Rickettsia, and rickettsial endosymbionts were identified in fleas infesting small mammals in the Košice region, Eastern Slovakia. This study is the first report of R. felis infection in C. solutus male flea collected from A. agrarius in Slovakia.

  12. IMMUNOHISTOCHEMISTRY VERSUS IMMUNOFLUORESENCE IN THE DIAGNOSIS OF AUTOIMMUNE BLISTERING DISEASES

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    Ana Maria Abreu Velez

    2013-11-01

    Full Text Available Introduction: In patients with autoimmune skin blistering diseases (ABDs, the diagnostic gold standard has classically been direct and indirect immunofluorescence (DIF and IIF, despite inherent technical problems of autofluorescence. Aim: We sought to overcome autofluorescence issues and compare the reliability of immunofluorescence versus immunohistochemistry (IHC staining in the diagnoses of these diseases. Methods: We tested via IHC for anti-human IgG, IgM, IgA, IgD, IgE, Kappa light chains, Lambda light chains, Complement/C3c, Complement/C1q, Complement/C3d, albumin and fibrinogen in 30 patients affected by a new variant of endemic pemphigus foliaceus in El Bagre, Colombia (El Bagre-EPF, and 30 control biopsies from the endemic area. We also tested archival biopsies from patients with ABDs whose diagnoses were made clinically, histopathologically and by DIF/IIF studies from 2 independent dermatopathology laboratories in the USA. Specifically, we tested 34 patients with bullous pemphigoid (BP, 18 with pemphigus vulgaris (PV, 8 with pemphigus foliaceus (PF, 14 with dermatitis herpetiformis (DH and 30 control skin samples from plastic esthetic surgery reduction surgeries. Results: The diagnostic correlation between IHC and DIF-IIF was almost 98% in most cases. IHC revealed evidence of autofluorescence around dermal blood vessels, dermal eccrine glands and neurovascular packages feeding skin appendices in ABDs; this autofluorescence may represent a non-specific immune response. Strong patterns of positivity were seen also in endothelial-mesenchymal cell junction-like structures, as well as between dermal fibrohistiocytic cells. In PV, we noted strong reactivity to neurovascular packages supplying sebaceous glands, as well as apocrine glands with edematous changes. Conclusions: We suggest that IHC is as reliable as DIF or IIF for the diagnosis of ABDs; our findings further suggest that what has previously been considered DIF/IIF autofluorescence

  13. Preliminary results of mercury levels in raw and cooked seafood and their public health impact.

    Science.gov (United States)

    Costa, Fernanda do N; Korn, Maria Graças A; Brito, Geysa B; Ferlin, Stacy; Fostier, Anne H

    2016-02-01

    Mercury is toxic for human health and one of the main routes of exposure is through consumption of contaminated fish and shellfish. The objective of this work was to assess the possible mercury contamination of bivalves (Anomalocardia brasiliana, Lucina pectinata, Callinectes sapidus), crustacean (C. sapidus) and fish (Bagre marinus and Diapterus rhombeus) collected on Salinas da Margarida, BA (Brazil), a region which carciniculture, fishing and shellfish extraction are the most important economic activities. The effect of cooking on Hg concentration in the samples was also studied. The results showed that Hg concentration was generally higher in the cooked samples than in raw samples. This increase can be related to the effect of Hg pre-concentration, formation of complexes involving mercury species and sulfhydryl groups present in tissues and/or loss of water and fat. The highest concentrations were found in B. marinus samples ranging 837.0-1585.3 μg kg(-1), which exceeded those recommended by Brazilian Health Surveillance Agency (ANVISA). In addition, Hg values found in the other samples also suggest the monitoring of the Hg concentrations in seafood consumed from the region.

  14. Estudio preliminar de ictioplancton de la Amazonia peruana con énfasis en la familia Pimelodidae

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    María Rojas

    2013-05-01

    Full Text Available En el presente trabajo se informa y describen las larvas de peces presentes en la naciente del río Madre de Dios, con énfasis en la familia Pimelodidae. Las colectas se realizaron entre noviembre y diciembre de 2004, utilizando una red de ictioplancton (300 micras de malla, en 8 transectos que cubrieron un tramo aproximado de 2 km aguas arriba y aguas abajo de la ciudad de Puerto Maldonado. Un total de 83750 larvas fueron examinadas, la mayor abundancia la presentaron los Characiformes (81%, seguido por Siluriformes (18%. En la familia Pimelodidae, se identificaron 12 especies de consumo, destacando Pseudoplatystoma tigrinum, P. fasciatum y Sorubim lima. Los principales órdenes encontrados en el ictioplancton siguen un patrón de abundancia relativa similar al estado adulto. La presencia de larvas de los grandes bagres migradores (familia Pimelodidae en la zona de muestreo sugiere que esta parte del río Madre de Dios es un área de reproducción, y por ende de importancia para la conservación de estas especies.

  15. A new species of Xyliphius, a rarely sampled banjo catfish (Siluriformes: Aspredinidae from the rio Tocantins-Araguaia system

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    Carlos A Figueiredo

    2010-03-01

    Full Text Available Xyliphius anachoretes, a new species of aspredinid catfish is described from the Tocantins-Araguaia River system. Xyliphius anachoretes is diagnosed by the presence of six developed retrorse serrae on posterior border of pectoral-fin spine, presence of papillae on the lower lip bearing minute branches, and only two dorsal procurrent rays. Comments about the informativeness of character-state variation among Xyliphius species and aspredinid related genera are furnished. Also, a brief discussion about conservation status of the new taxon is made.Xyliphius anachoretes, uma nova espécie de bagre aspredinídeo é descrita para o sistema Tocantins-Araguaia. Xyliphius anachoretes é diagnosticado pela presença de seis serras retrorsas desenvolvidas na borda posterior do espinho da nadadeira peitoral, presença de papilas no lábio inferior apresentando minúsculas ramificações, e somente dois raios pró-correntes dorsais. Comentários sobre a informação contida na variação de estados de caráter entre as espécies de Xyliphius são fornecidos. Além disso, uma breve discussão sobre o estado de conservação do novo táxon é feita.

  16. [Impact of water resource installations on the distribution of schistosomiasis and its intermediary hosts in Burkina Faso].

    Science.gov (United States)

    Poda, Jean-Noël; Sondo, Blaise; Parent, Gérard

    2003-01-01

    Dams generally are a favourable biotope for the molluscs acting as intermediary hosts to schistosomiasis. The importance of the schistosomiasis endemic which follows depends on the interactions taking place between the parasites and their definitive (humans) and intermediary hosts. A preliminary sound knowledge of the prevailing epidemiological situations is therefore necessary to define an efficient programme to fight these infections. The extension of schistosomiasis following the installation of water resource facilities is significative of the part played by these hosts. In the hydroagricultural complex of Sourou, the prevalence of urinary schistosomiasis increased from 19% in 1954 to more than 70% in 1998-1999 in Guiédougou, the most ancient site. As to digestive schistosomiasis, almost unheard of until 1987, its prevalence ranged from 8% to 69% in 1998 in the villages located alongside the areas thus equipped. In the Kou Valley, the prevalence went up from 14% in 1957 to 80% in 1974 for urinary schistosomiasis and from 1.3% to 45% for intestinal schistosomiasis. The same tendencies are likely to appear in the hydraulic installations of Bagré, Ziga, and Kompienga. Dams thus constitute amplifying factors for the proliferation of species and for parasite-host interactions. All the actors (developers, populations and scientists) are faced with the challenge of finding a mean to control the development of schistosomiasis infections which are likely to seriously lessen the benefits expected from these hydraulic installations.

  17. Feeding ecology of Auchenipterichthys longimanus (Siluriformes: Auchenipteridae in a riparian flooded forest of Eastern Amazonia, Brazil

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    Tiago Magalhães da Silva Freitas

    2011-01-01

    Full Text Available Feeding habits of the midnight catfish Auchenipterichthys longimanus collected in rivers of the Caxiuanã National Forest (Eastern Amazonia, Brazil were investigated through the different hydrological periods (dry, filing, flood and drawdown. A total of 589 specimens were collected throughout seven samplings between July 2008 and July 2009, of which 74 were young males, 177 adult males, 89 young females and 249 adult females. The diet composition (Alimentary index - Ai% was analyzed by a non-metric multidimensional scaling (nMDS and by the analysis of similarity (ANOSIM, which included 37 items grouped into nine categories (Aquatic insects, Other aquatic invertebrates, Arthropods fragment, Fish, Plant fragment, Seeds, Terrestrial insects, Other terrestrial invertebrates, and Terrestrial vertebrates. We also calculated the niche breadth (Levins index and the repletion index (RI%. Differences in the diet composition between hydrological seasons were registered, primarily on diet composition between dry and flood season, but changes related with sex and maturity were not observed. The midnight catfish showed more specialists feeder habit in the flood period (March 2009 and more generalist habits in the dry season (November 2008. The amount of food eaten by A. longimanus based on repletion index (RI%, did not differ significantly from sex and maturity. However, we evidenced differences in RI% when comparing the studied months. These results provide important biological information about the trophic ecology of auchenipterids fish. In view of the higher occurrence of allochthonous items, this research also underpins the importance of riparian forests as critical environments in the maintenance and conservation of wild populations of fish in the Amazon basin.Neste estudo foram investigados os hábitos alimentares do bagre Auchenipterichthys longimanus coletados em rios da Floresta Nacional de Caxiuanã (Amazônia Oriental, Brasil ao longo de diferentes

  18. TISSUE INHIBITOR OF METALLOPROTEINASE 1, MATRIX METALLOPROTEINASE 9, ALPHA-1 ANTITRYPSIN, METALLOTHIONEIN AND UROKINASE TYPE PLASMINOGEN ACTIVATOR RECEPTOR IN SKIN BIOPSIES FROM PATIENTS AFFECTED BY AUTOIMMUNE BLISTERING DISEASES

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    Ana Maria Abreu Velez

    2013-07-01

    Full Text Available Introduction: Proteinases and proteinase inhibitors have been described to play a role in autoimmune skin blistering diseases. We studied skin lesional biopsies from patients affected by several autoimmune skin blistering diseases for proteinases and proteinase inhibitors. Methods: We utilized immunohistochemistry to evaluate biopsies for alpha-1-antitrypsin, human matrix metalloproteinase 9 (MMP9, human tissue inhibitor of metalloproteinases 1 (TIMP-1, metallothionein and urokinase type plasminogen activator receptor (uPAR. We tested 30 patients affected by endemic pemphigus, 30 controls from the endemic area, and 15 normal controls. We also tested 30 biopsies from patients with bullous pemphigoid (BP, 20 with pemphigus vulgaris (PV, 8 with pemphigus foliaceus, and 14 with dermatitis herpetiformis (DH. Results: Contrary to findings in the current literature, most autoimmune skin blistering disease biopsies were negative for uPAR and MMP9. Only some chronic patients with El Bagre-EPF were positive to MMP9 in the dermis, in proximity to telocytes. TIMP-1 and metallothionein were positive in half of the biopsies from BP patients at the basement membrane of the skin, within several skin appendices, in areas of dermal blood vessel inflammation and within dermal mesenchymal-epithelial cell junctions.

  19. Malaria in gold-mining areas in Colombia.

    Science.gov (United States)

    Castellanos, Angélica; Chaparro-Narváez, Pablo; Morales-Plaza, Cristhian David; Alzate, Alberto; Padilla, Julio; Arévalo, Myriam; Herrera, Sócrates

    2016-01-01

    Gold-mining may play an important role in the maintenance of malaria worldwide. Gold-mining, mostly illegal, has significantly expanded in Colombia during the last decade in areas with limited health care and disease prevention. We report a descriptive study that was carried out to determine the malaria prevalence in gold-mining areas of Colombia, using data from the public health surveillance system (National Health Institute) during the period 2010-2013. Gold-mining was more prevalent in the departments of Antioquia, Córdoba, Bolívar, Chocó, Nariño, Cauca, and Valle, which contributed 89.3% (270,753 cases) of the national malaria incidence from 2010-2013 and 31.6% of malaria cases were from mining areas. Mining regions, such as El Bagre, Zaragoza, and Segovia, in Antioquia, Puerto Libertador and Montelíbano, in Córdoba, and Buenaventura, in Valle del Cauca, were the most endemic areas. The annual parasite index (API) correlated with gold production (R2 0.82, p gold produced, the API increased by 0.54 cases per 1,000 inhabitants. Lack of malaria control activities, together with high migration and proliferation of mosquito breeding sites, contribute to malaria in gold-mining regions. Specific control activities must be introduced to control this significant source of malaria in Colombia. PMID:26814645

  20. Malaria in gold-mining areas in Colombia

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    Angélica Castellanos

    2016-01-01

    Full Text Available Gold-mining may play an important role in the maintenance of malaria worldwide. Gold-mining, mostly illegal, has significantly expanded in Colombia during the last decade in areas with limited health care and disease prevention. We report a descriptive study that was carried out to determine the malaria prevalence in gold-mining areas of Colombia, using data from the public health surveillance system (National Health Institute during the period 2010-2013. Gold-mining was more prevalent in the departments of Antioquia, Córdoba, Bolívar, Chocó, Nariño, Cauca, and Valle, which contributed 89.3% (270,753 cases of the national malaria incidence from 2010-2013 and 31.6% of malaria cases were from mining areas. Mining regions, such as El Bagre, Zaragoza, and Segovia, in Antioquia, Puerto Libertador and Montelíbano, in Córdoba, and Buenaventura, in Valle del Cauca, were the most endemic areas. The annual parasite index (API correlated with gold production (R2 0.82, p < 0.0001; for every 100 kg of gold produced, the API increased by 0.54 cases per 1,000 inhabitants. Lack of malaria control activities, together with high migration and proliferation of mosquito breeding sites, contribute to malaria in gold-mining regions. Specific control activities must be introduced to control this significant source of malaria in Colombia.

  1. Malaria in gold-mining areas in Colombia.

    Science.gov (United States)

    Castellanos, Angélica; Chaparro-Narváez, Pablo; Morales-Plaza, Cristhian David; Alzate, Alberto; Padilla, Julio; Arévalo, Myriam; Herrera, Sócrates

    2016-01-01

    Gold-mining may play an important role in the maintenance of malaria worldwide. Gold-mining, mostly illegal, has significantly expanded in Colombia during the last decade in areas with limited health care and disease prevention. We report a descriptive study that was carried out to determine the malaria prevalence in gold-mining areas of Colombia, using data from the public health surveillance system (National Health Institute) during the period 2010-2013. Gold-mining was more prevalent in the departments of Antioquia, Córdoba, Bolívar, Chocó, Nariño, Cauca, and Valle, which contributed 89.3% (270,753 cases) of the national malaria incidence from 2010-2013 and 31.6% of malaria cases were from mining areas. Mining regions, such as El Bagre, Zaragoza, and Segovia, in Antioquia, Puerto Libertador and Montelíbano, in Córdoba, and Buenaventura, in Valle del Cauca, were the most endemic areas. The annual parasite index (API) correlated with gold production (R2 0.82, p Colombia.

  2. Malaria in gold-mining areas in Colombia

    Science.gov (United States)

    Castellanos, Angélica; Chaparro-Narváez, Pablo; Morales-Plaza, Cristhian David; Alzate, Alberto; Padilla, Julio; Arévalo, Myriam; Herrera, Sócrates

    2016-01-01

    Gold-mining may play an important role in the maintenance of malaria worldwide. Gold-mining, mostly illegal, has significantly expanded in Colombia during the last decade in areas with limited health care and disease prevention. We report a descriptive study that was carried out to determine the malaria prevalence in gold-mining areas of Colombia, using data from the public health surveillance system (National Health Institute) during the period 2010-2013. Gold-mining was more prevalent in the departments of Antioquia, Córdoba, Bolívar, Chocó, Nariño, Cauca, and Valle, which contributed 89.3% (270,753 cases) of the national malaria incidence from 2010-2013 and 31.6% of malaria cases were from mining areas. Mining regions, such as El Bagre, Zaragoza, and Segovia, in Antioquia, Puerto Libertador and Montelíbano, in Córdoba, and Buenaventura, in Valle del Cauca, were the most endemic areas. The annual parasite index (API) correlated with gold production (R2 0.82, p < 0.0001); for every 100 kg of gold produced, the API increased by 0.54 cases per 1,000 inhabitants. Lack of malaria control activities, together with high migration and proliferation of mosquito breeding sites, contribute to malaria in gold-mining regions. Specific control activities must be introduced to control this significant source of malaria in Colombia. PMID:26814645

  3. Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips

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    Silvia Tamie Matsumoto

    2006-01-01

    Full Text Available Cytotoxicity of metals is important because some metals are potential mutagens able to induce tumors in humans and experimental animals. Chromium can damage DNA in several ways, including DNA double strand breaks (DSBs which generate chromosomal aberrations, micronucleus formation, sister chromatid exchange, formation of DNA adducts and alterations in DNA replication and transcription. In our study, water samples from three sites in the Córrego dos Bagres stream in the Franca municipality of the Brazilian state of São Paulo were subjected to the comet assay and micronucleus test using erythrocytes from the fish Oreochromis niloticus. Nuclear abnormalities of the erythrocytes included blebbed, notched and lobed nuclei, probably due to genotoxic chromium compounds. The greatest comet assay damage occurred with water from a chromium-containing tannery effluent discharge site, supporting the hypothesis that chromium residues can be genotoxic. The mutagenicity of the water samples was assessed using the onion root-tip cell assay, the most frequent chromosomal abnormalities observed being: c-metaphases, stick chromosome, chromosome breaks and losses, bridged anaphases, multipolar anaphases, and micronucleated and binucleated cells. Onion root-tip cell mutagenicity was highest for water samples containing the highest levels of chromium.

  4. Molecular characterization of Cryptosporidium spp. from HIV infected patients from an urban area of Brazil Caracterização molecular de Cryptosporidium spp. de pacientes de área urbana do Brasil infectados por HIV

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    Patrícia de Lucca

    2009-12-01

    Full Text Available Cryptosporidium spp. are important cause of enteric disease in humans, but may also infect animals. This study describes the relative frequency of several Cryptosporidium species found in human specimens from HIV infected patients in the São Paulo municipality obtained from January to July 2007. Sequence analysis of the products of nested-PCR based on small subunit rRNA and Cryptosporidium oocyst wall protein coding genes revealed 17 (63.0% isolates of C. hominis, four (14.8% C. parvum, five (18.5% C. felis and one (3.7% C. canis. These findings suggest that, in urban environments of Brazil, the cat adapted C. felis may play a potential role in the zoonotic transmission of cryptosporidiosis whereas the anthroponotic transmission of cryptosporidiosis caused by C. hominis seems to predominate.Cryptosporidium spp. são importantes causas de doenças entéricas em humanos, mas podem também ser encontrados em animais. O presente estudo descreve a frequência relativa de diversas espécies de Cryptosporidium em amostras de humanos da cidade de São Paulo, Brasil, obtidas de janeiro a julho de 2007. Análises de sequências de produtos de nested PCR direcionadas ao genes codificadores da menor unidade ribosomal e da proteina de parede de oocistos revelaram 17 (63,0% isolados de C. hominis, quatro (14,8% C. parvum, cinco (18,5% C. felis, e um (3,7% C. canis. Estes resultados sugerem que, em ambientes urbanos no Brasil, o genótipo adaptado ao gato pode desempenhar potencial papel na transmissão zoonótica de criptosporidiose, enquanto a transmissão antroponótica da criptosporidiose causada pelo C. hominis parece predominar.

  5. Aerobic bacteria from mucous membranes, ear canals, and skin wounds of feral cats in Grenada, and the antimicrobial drug susceptibility of major isolates.

    Science.gov (United States)

    Hariharan, Harry; Matthew, Vanessa; Fountain, Jacqueline; Snell, Alicia; Doherty, Devin; King, Brittany; Shemer, Eran; Oliveira, Simone; Sharma, Ravindra N

    2011-03-01

    In a 2-year period 54 feral cats were captured in Grenada, West Indies, and a total of 383 samples consisting of swabs from rectum, vagina, ears, eyes, mouth, nose and wounds/abscesses, were cultured for aerobic bacteria and campylobacters. A total of 251 bacterial isolates were obtained, of which 205 were identified to species level and 46 to genus level. A commercial bacterial identification system (API/Biomerieux), was used for this purpose. The most common species was Escherichia coli (N=60), followed by Staphylococcus felis/simulans (40), S. hominis (16), S. haemolyticus (12), Streptococcus canis (9), Proteus mirabilis (8), Pasteurella multocida (7), Streptococcus mitis (7), Staphylococcus xylosus (7), S. capitis (6), S. chromogenes (4), S. sciuri (3), S. auricularis (2), S. lentus (2), S. hyicus (2), Streptococcus suis (2) and Pseudomonas argentinensis (2). Sixteen other isolates were identified to species level. A molecular method using 16S rRNA sequencing was used to confirm/identify 22 isolates. Salmonella or campylobacters were not isolated from rectal swabs. E. coli and S. felis/simulans together constituted 50% of isolates from vagina. S. felis/simulans was the most common species from culture positive ear and eye samples. P. multocida was isolated from 15% of mouth samples. Coagulase-negative staphylococci were the most common isolates from nose and wound swabs. Staphylococcus aureus, or S. intemedius/S. pseudintermedius were not isolated from any sample. Antimicrobial drug resistance was minimal, most isolates being susceptible to all drugs tested against, including tetracycline. PMID:20627391

  6. The dog louse Heterodoxus spiniger from stray cats in Penang, Malaysia.

    Science.gov (United States)

    Norhidayu, S; Mohd Zain, S N; Jeffery, J; Lewis, J W

    2012-06-01

    Stray cats collected from Georgetown, Penang from 2008 to 2010 were screened for ectoparasites via fine-tooth combing. Two cats from a total 102 examined were infested with the dog louse, Heterodoxus spiniger. Both cats, a juvenile male and female were found in close contact with each other prior to capture. The number of lice ranged from 5 and 14 in the male and female cat respectively. Other ectoparasites recovered included the common cat flea, Ctenocephalides felis, one louse species Felicola subrostratus, one tick species Haemaphysalis bispinosa and one mite species of Listrophoridae. The present study reports for the first time the finding of H. spiniger on cats from peninsular Malaysia.

  7. Dicty_cDB: AHA677 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ;, mRNA sequence. 66 4e-15 3 BM056853 |BM056853.1 2154-85 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides... |BM058379.1 2191-29 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone ...2191-29, mRNA sequence. 90 7e-14 1 BM058578 |BM058578.1 2193-94 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides

  8. EST Table: DC550282 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DC550282 E_FL_phe-_20L24_F_0 10/09/28 38 %/139 aa gb|AAD21841.1| trypsin-like serine protease [Ctenocephalid...es felis] 10/09/02 n.h 10/08/28 n.h 10/09/10 32 %/143 aa AGAP004318-PA Protein|2R:54406026:54412612:-1|gene:CLIPC3 10/09/10 n.h 10/09/10 n.h FS930815 phe- ...

  9. EST Table: FY738641 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY738641 E_FL_famL_06F07_F_0 11/11/04 39 %/172 aa gb|AAD21841.1| trypsin-like serine protease [Ctenocephalid...es felis] 11/11/04 n.h 11/11/04 n.h 11/11/04 32 %/142 aa AGAP004318-PA Protein|2R:54406026:54412612:-1|gene:CLIPC3 11/11/04 n.h 11/11/04 n.h FS930815 famL ...

  10. Freqüência de parasitas intestinais em felinos mantidos em zoológicos Frequency of intestinal parasites in felines kept in zoos

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    G.C.K. Müller

    2005-08-01

    Full Text Available The endoparasites occurrence in felines confined in two Zoos, between December 1999 and April 2000, was studied. Fecal samples of 18 felines (Panthera tigris, Panthera leo, Felis serval, Panthera onca, Puma concolor, Leopardus tigrinus and Leopardus wiedii were collected and the methods of Faust, modified Baermann and Hoffmann, were used for fecal analyses. Three genera were identified in the feces: Trichuris spp., Toxocara spp. and Giardia spp. In the zoo of Pomerode, six animals (46% were infected by Trichuris spp. and/or Giardia spp. and all samples from the zoo of Brusque were infected by Trichuris spp., Toxocara spp. and Giardia spp.

  11. Sarcocystis leporum in cottontail rabbits and its transmission to carnivores.

    Science.gov (United States)

    Fayer, R; Kradel, D

    1977-04-01

    Muscle from Sarcocystis-infected cottontail rabbits (Sylvilagus floridanus) was fed to coccidia-free cats (Felis domestica) and dogs (Canis familiaris). Only cats became infected and shed sporocysts in their feces. The prepatent period ranged from 10 to 25 days and the patent period from 3 to 46 days. Sporocysts were fully sporulated when shed. They contained 4 sporozoites and a coarse granular residuum and averaged 9.4 by 13.6 micron (N=55). Doses of 200-75,000 sporocysts were orally administered to 5 domestic rabbits (Oryctolagus cuniculus). Domestic rabbits did not become infected, suggesting a strict host specificity for the intermediate host S. floridanus. PMID:405509

  12. Sarcocystis and related organisms in Australian Wildlife: IV. Studies on Sarcocystis cuniculi in European rabbits (Oryctolagus cuniculus).

    Science.gov (United States)

    Munday, B L; Smith, D D; Frenkel, J K

    1980-04-01

    The role of the cat (Felis domestica) as a definitive host for Sarcocystis cuniculi of European rabbits (Oryctolagus cuniculus) was confirmed. It was shown that after dosing with sporocysts from cats, rabbits developed sarcocysts and these became infective for cats at not less than 93 days post-infection (p.i.). The earliest infection detected was at 142 days p.i. Infected muscle from an experimental rabbit did not transmit Sarcocystis when fed to other rabbits. Microscopically, sarcocysts in European rabbits (O. cuniculus) were morphologically indistinguishable from those in cottontail rabbits (Sylvilagus floridanus). PMID:6776293

  13. Información proporcionada por cazadores sobre la fauna de Vadocondes (Burgos) el 13 de noviembre de 1953

    OpenAIRE

    Valverde Gómez, José Antonio, 1926-2003

    2008-01-01

    Información proporcionada al autor por cazadores, sobre la fauna de Vadocondes (Burgos), a 13 de noviembre de 1953, que incluye a los siguientes anfibios: Alytes sp. (Sapo partero), Bufo sp. (Sapo) y "Molge sp." (puede referirse a Triturus marmoratus o a Pleurodeles waltl), los siguientes mamíferos: Ardilla (pudiendo ser, Sciurus vulgaris o Atlantoxerus getulus), Canis lupus (Lobo), Erizo (pudiendo ser Erinaceus europaeus o Atelerix algirus), Felis silvestris (Gato montés), Lirón (pudiendo se...

  14. Determinants of FIV and HIV Vif sensitivity of feline APOBEC3 restriction factors

    OpenAIRE

    Zhang, Zeli; Gu, Qinyong; Jaguva Vasudevan, Ananda Ayyappan; Hain, Anika; Kloke, Björn-Philipp; Hasheminasab, Sascha; Mulnaes, Daniel; Sato, Kei; Cichutek, Klaus; Häussinger, Dieter; Bravo, Ignacio G; Smits, Sander H. J.; Gohlke, Holger; Münk, Carsten

    2016-01-01

    Background Feline immunodeficiency virus (FIV) is a global pathogen of Felidae species and a model system for Human immunodeficiency virus (HIV)-induced AIDS. In felids such as the domestic cat (Felis catus), APOBEC3 (A3) genes encode for single-domain A3Z2s, A3Z3 and double-domain A3Z2Z3 anti-viral cytidine deaminases. The feline A3Z2Z3 is expressed following read-through transcription and alternative splicing, introducing a previously untranslated exon in frame, encoding a domain insertion ...

  15. Étude d'une grotte à Ours du Pléistocène moyen en Dordogne : la Grotte XIV.

    OpenAIRE

    Guadelli, Jean-Luc

    1997-01-01

    La Grotte XIV (Dordogne, France) a livré des restes attribuables à des espèces d'âge pléistocène moyen ancien : Dinobatis, Panthera gombaszoegensis, Panthère, Felis (de grande taille) ou Lynx (de petite taille), Ursus cf. deningeri, Canis cf. etruscus, Hemitragus, Cervus, Capreolus, Dicerorhinus,... Les restes d'Ours présentent des caractères très primitifs (entoconide des M1 très simple, faible torsion de l'articulation distale des tibias, ...) ce qui pose la question de l'identification pré...

  16. Efecto clínico de la inyección intramuscular de alfaxalona sola o en combinación con dexmedetomidina y estudio farmacocinético-farmacodinámico en la especie felina

    OpenAIRE

    Rodrigo Mocholí, Diego

    2016-01-01

    Los objetivos planteados en esta Tesis son: • Estudiar el efecto sedante y/o anestésico de la alfaxalona administrada sola o en combinación con dexmedetomidina por vía intramuscular en la especie felina. • Determinar los efectos a nivel cardiorrespiratorio observados en gatos tras el empleo intramuscular de estas combinaciones. • Efectuar un estudio farmacocinético y farmacodinámico comparativo tras la administración de alfaxalona por vía intramuscular y endovenosa en la especie feli...

  17. Evolutionary conservation of ten microsatellite loci in four species of Felidae.

    Science.gov (United States)

    Menotti-Raymond, M A; O'Brien, S J

    1995-01-01

    Short tandem repeat polymorphisms (STRP), or microsatellites, are widespread among vertebrate genomes and are useful in gene mapping and population studies due to a high level of length polymorphism. We describe here the isolation, characterization, and PCR amplification of 10 microsatellite loci from the domestic cat, Felis catus. The flanking primer sequences were conserved among other Felidae species, and amplification products demonstrated abundant polymorphism in puma, lion, cheetah, and domestic cat. The cheetah sample exhibited the lowest level of polymorphism for these loci among felid species. PMID:7658003

  18. Dicty_cDB: VSD185 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available and Malpighian tubule cDNA library Ctenocephalides felis cDNA clone 3254-25, mRNA sequence. 48 2e-06 2 BQ476622 |BQ476622.1 curculio...3e06.g Curculio glandium cDNA Curculio glandium cDNA clone curculio...3e06 3', mRNA sequence. 46 0.46 1 BQ476340 |BQ476340.1 curculio3e06.b Curculio glandium cDNA Curc...ulio glandium cDNA clone curculio3e06 5', mRNA sequence. 46 0.46 1 AX345182 |AX345182.1 Sequence 253 from Pa

  19. Ecología e impacto de los mamíferos invasores en ecosistemas insulares: la isla de Corvo, Archipiélago de las Azores

    OpenAIRE

    Hervías Parejo, Sandra

    2014-01-01

    Tesis por compendio de publicaciones Esta investigación se ha centrado en analizar el impacto directo e indirecto de los tres mamíferos invasores más comunes en los ecosistemas insulares (el ratón casero Mus domesticus, la rata negra Rattus rattus y el gato Felis silvestris catus), sobre la pardela cenicienta (Calonectris diomedea borealis). El trabajo se ha desarrollado en Corvo, que es la isla más remota del Archipiélago de las Azores y la última en ser descubierta y colonizada, por lo q...

  20. Dicty_cDB: Contig-U10274-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available :none) Burkholderia vietnamiensis G4 c... 75 2e-12 CR378672_161( CR378672 |pid...:none) Methanosarcina mazei strain Goe1... 89 1e-16 CP000053_198( CP000053 |pid:none) Rickettsia felis URRWXCal2, co...romyxobacter sp. K, complete... 87 7e-16 AL939131_102( AL939131 |pid:none) Streptomyces coelicolor A3(2) co... 3500... 79 1e-13 AL939112_114( AL939112 |pid:none) Streptomyces coelicolor A3(2) co....la str. Sg (Schizaphis graminum)... 44 7.1 1 >( C94358 ) Dictyostelium discoideum slug

  1. Assessing Risks to Non-Target Species during Poison Baiting Programs for Feral Cats

    OpenAIRE

    Tony Buckmaster; Dickman, Christopher R.; Johnston, Michael J

    2014-01-01

    Poison baiting is used frequently to reduce the impacts of pest species of mammals on agricultural and biodiversity interests. However, baiting may not be appropriate if non-target species are at risk of poisoning. Here we use a desktop decision tree approach to assess the risks to non-target vertebrate species in Australia that arise from using poison baits developed to control feral house cats (Felis catus). These baits are presented in the form of sausages with toxicant implanted in the ba...

  2. A Case of Human Infection by Rickettsia slovaca in Greece.

    Science.gov (United States)

    Kostopoulou, Vasiliki; Chochlakis, Dimosthenis; Kanta, Chrysoula; Katsanou, Andromachi; Rossiou, Konstantina; Rammos, Aidonis; Papadopoulos, Spyridon-Filippos; Katsarou, Theodora; Tselentis, Yannis; Psaroulaki, Anna; Boukas, Chrysostomos

    2016-07-22

    Although tick-borne rickettsiosis is endemic in Greece, until recently, human samples arriving at the National Reference Centre under suspicion of rickettsial infection were routinely tested only for Rickettsia typhi and R. conorii. However, identification of additional rickettsia species in ticks prompted revision of the protocol in 2010. Until that year, all human samples received by the laboratory were tested for antibodies against R. conorii and R. typhi only. Now, tests for R. slovaca, R. felis, and R. mongolotimonae are all included in routine analysis. The current description of a human R. slovaca case is possible as a result of these changes in routine testing. PMID:26370429

  3. Efficacy of an imidacloprid/flumethrin collar against fleas and ticks on cats

    Directory of Open Access Journals (Sweden)

    Stanneck Dorothee

    2012-04-01

    Full Text Available Abstract Background The objectives of the studies listed here were to ascertain the therapeutic and sustained efficacy of 10% imidacloprid (w/w and 4.5% flumethrin (w/w incorporated in a slow-release matrix collar, against laboratory-infestations of fleas and ticks on cats. Efficacy was evaluated against the flea Ctenocephalides felis felis, and the ticks Ixodes ricinus, Amblyomma americanum and Rhipicephalus turanicus. The number of studies was so large that only a general overview can be presented in this abstract. Methods Preventive efficacy was evaluated by infesting groups of cats (n = 8-10 with C. felis felis and/or I. ricinus, A. americanum or R. turanicus at monthly intervals at least, for a period of up to 8 months. Efficacy against fleas was evaluated 24 to 48 h after treatment and 24 h after infestation, and against ticks at 6 h (repellent or 48 h (acaricidal after infestation. Efficacy against flea larvae was evaluated over a period of 8 months by incubating viable flea eggs on blanket samples after cat contact. In all cases efficacy was calculated by comparison with untreated negative control groups. Results Efficacy against fleas (24 h generally exceeded 95% until study termination. In vitro efficacy against flea larvae exceeded 92% until Day 90 and then declined to 67% at the conclusion of the study on Day 230. Sustained acaricidal (48 h efficacy over a period of eight months was consistently 100% against I. ricinus from Day 2 after treatment, 100% against A. americanum, except for 98.5% and 97.7% at two time-points, and between 94% and 100% against R. turanicus. From Day 2 until 8 months after treatment the repellent (6 h, efficacy was consistently 100% against I. ricinus, and between 54.8% and 85.4% against R. turanicus. Conclusion The rapid insecticidal and acaricidal properties of the medicated collars against newly- acquired infestations of fleas and ticks and their sustained high levels of preventive efficacy have been

  4. Discovery of sarolaner: A novel, orally administered, broad-spectrum, isoxazoline ectoparasiticide for dogs.

    Science.gov (United States)

    McTier, Tom L; Chubb, Nathan; Curtis, Michael P; Hedges, Laura; Inskeep, Gregory A; Knauer, Christopher S; Menon, Sanjay; Mills, Brian; Pullins, Aleah; Zinser, Erich; Woods, Debra J; Meeus, Patrick

    2016-05-30

    The novel isoxazoline ectoparasiticide, sarolaner, was identified during a lead optimization program for an orally-active compound with efficacy against fleas and ticks on dogs. The aim of the discovery program was to identify a novel isoxazoline specifically for use in companion animals, beginning with de novo synthesis in the Zoetis research laboratories. The sarolaner molecule has unique structural features important for its potency and pharmacokinetic (PK) properties, including spiroazetidine and sulfone moieties. The flea and tick activity resides in the chirally pure S-enantiomer, which was purified to alleviate potential off-target effects from the inactive enantiomer. The mechanism of action was established in electrophysiology assays using CHO-K1 cell lines stably expressing cat flea (Ctenocephalides felis) RDL (resistance-to-dieldrin) genes for assessment of GABA-gated chloride channel (GABACls) pharmacology. As expected, sarolaner inhibited GABA-elicited currents at both susceptible (CfRDL-A285) and resistant (CfRDL-S285) flea GABACls with similar potency. Initial whole organism screening was conducted in vitro using a blood feeding assay against C. felis. Compounds which demonstrated robust activity in the flea feed assay were subsequently tested in an in vitro ingestion assay against the soft tick, Ornithodoros turicata. Efficacious compounds which were confirmed safe in rodents at doses up to 30mg/kg were progressed to safety, PK and efficacy studies in dogs. In vitro sarolaner demonstrated an LC80 of 0.3μg/mL against C. felis and an LC100 of 0.003μg/mL against O. turicata. In a head-to-head comparative in vitro assay with both afoxolaner and fluralaner, sarolaner demonstrated superior flea and tick potency. In exploratory safety studies in dogs, sarolaner demonstrated safety in dogs≥8 weeks of age upon repeated monthly dosing at up to 20mg/kg. Sarolaner was rapidly and well absorbed following oral dosing. Time to maximum plasma concentration

  5. Al este de la campana de Gauss. Una aproximación desde la docencia a las altas capacidades. Aprendizajes significativos desde su hipersensibilidad e hiperestesia

    OpenAIRE

    Fernández Bascaran, Eva

    2013-01-01

    Mi investigación se basa en tratar de obtener aprendizajes significativos en alumnado con altas capacidades tomando como aliado a su favor su hipersensibilidad e hiperestesia, su alto sentido de la justicia y su empatía. Investigar la habilidad del alumnado con altas capacidades no solo en el plano cognitivo, sino también desde el plano sensitivo-afectivo-emocional. Utilizar su hipersensibilidad y empatía para ayudar a sus compañeros (aprendizaje cooperativo) y reforzar su personalidad y feli...

  6. Isolation of Microsporum gypseum from the haircoat of health wild felids kept in captivity in Brazil

    OpenAIRE

    Bentubo Henri Donnarumma Levy; Fedullo José Daniel Luzes; Corrêa Sandra Helena Ramiro; Teixeira Rodrigo Hidalgo Friciello; Coutinho Selene Dall' Acqua

    2006-01-01

    Dermatophytes are fungi that cause superficial mycoses in animals and humans. While studies have shown that domestic cats (Felis catus) are often asymptomatic carriers of dermatophytes, and thus a significant source of infection, this aspect has not been studied in relation to their wild relatives. The present study was aimed at determining the presence of dermatophytes on the haircoat of healthy wild felids, kept in captivity at "Fundação Parque Zoológico de São Paulo". Samples were taken fr...

  7. Isolation of Microsporum gypseum from the haircoat of health wild felids kept in captivity in Brazil Isolamento de Microsporum gypseum do pelame de felídeos selvagens sadios mantidos em cativeiro no Brasil

    OpenAIRE

    Henri Donnarumma Levy Bentubo; José Daniel Luzes Fedullo; Sandra Helena Ramiro Corrêa; Rodrigo Hidalgo Friciello Teixeira; Selene Dall' Acqua Coutinho

    2006-01-01

    Dermatophytes are fungi that cause superficial mycoses in animals and humans. While studies have shown that domestic cats (Felis catus) are often asymptomatic carriers of dermatophytes, and thus a significant source of infection, this aspect has not been studied in relation to their wild relatives. The present study was aimed at determining the presence of dermatophytes on the haircoat of healthy wild felids, kept in captivity at "Fundação Parque Zoológico de São Paulo". Samples were taken fr...

  8. N-acetylcysteine, a novel treatment for Helicobacter pylori infection.

    Science.gov (United States)

    Huynh, Hien Quoc; Couper, Richard T L; Tran, Cuong D; Moore, Lynette; Kelso, Richard; Butler, Ross N

    2004-01-01

    N-Acetylcysteine (NAC), being both a mucolytic agent and a thiol-containing antioxidant, may affect the establishment and maintenance of H. pylori infection within the gastric mucus layer and mucosa. Agar and broth dilution susceptibility tests determined the MIC of H. pylori strain SSI to NAC. H. pylori load in SSI strain-infected C57BL mice was determined as colony forming units per gram of gastric tissue. Gastritis assessment was scored and gastric surface hydrophobicity was determined by contact angle measurement. MICs of NAC were 5 to 10 and 10 to 15 mg/ml using the agar dilution and broth dilution methods, respectively. NAC (120 mg per day for 14 days) reduced the H. pylori load in mice by almost 1 log compared with sham treatment. Pretreatment with NAC (40 mg/day) also significantly reduced the H. pylori load but did not prevent H. pylori colonization. Both H. pylori infection and NAC reduced the surface hydrophobicity of murine gastric mucosa. No significant differences were observed in the gastritis scores of H. felis- or H. pylori-infected mice receiving either NAC or sham treatments. This study demonstrates that NAC inhibits the growth of H. pylori in both agar and broth susceptibility tests and in H. pylori-infected mice. NAC did not alter the severity of H. pylori- or H. felis-induced gastritis.

  9. Ectoparasites of Nasua nasua (Carnivora, Procyonidae from an urban forest in Southeastern Brazil Ectoparasitos de quatis Nasua nasua (Carnivora, Procyonidae em uma floresta urbana no sudeste brasileiro

    Directory of Open Access Journals (Sweden)

    A.F.S.F. Rodrigues

    2006-10-01

    Full Text Available Em um fragmento de mata na área urbana de Juiz de Fora (MG foram capturados 15 quatis com armadilha e ceva, para estudo dos seus ectoparasitos. Outros quatro animais, atropelados no entorno, foram também examinados imediatamente após o atropelamento, e incluídos na análise. Os ectoparasitos foram removidos com a utilização de pinça e pente-fino e acondicionados em etanol 70°GL. Pulgas e piolhos foram clarificados e montados para análise em microscopia. Os ixodídeos foram identificados sob estereoscopia. Não foram encontrados carrapatos adultos. Larvas e ninfas de carrapatos foram encontradas, respectivamente, em 36,8% e 63,1% dos hospedeiros examinados. Ninfas que sofreram muda foram identificadas como Amblyomma cajenennse. A espécie de piolho Neotrichodectes pallidus foi obtida em 52,6% dos quatis. As pulgas Ctenocephalides felis felis e Rhopalopsyllus lutzi lutzi apresentaram, respectivamente, as seguintes prevalências: 36,8% e 35,1%. O estudo mostra que no fragmento de mata na área urbana os quatis podem manter espécies de ectoparasitos comuns a estes hospedeiros, bem como intercambia-las entre o ambiente silvestre e urbano.

  10. Cat, cougar, and jaguar spermatogenesis: a comparative analysis

    Directory of Open Access Journals (Sweden)

    Deiler Sampaio Costa

    2006-09-01

    Full Text Available This work presents a comparative review about the spermatogenic process in cats (Felis domestica, jaguars (Panthera onca and cougars (Puma concolor, with emphasis on testicular biometry, gonadossomatic index, volumetric proportion of testicular parenchyma components, tubular diameter, seminiferous epithelial height and seminiferous tubule length. It was an approach of the differences among the cell proportions that allowed conclusions about the overall yield of spermatogenic process and Sertoli cell index in three feline species.O processo espermatogênico do gato doméstico (Felis domestica, da onça-pintada (Panthera onca e da onça-parda (Puma concolor são analisados de forma comparativa, dando-se ênfase à biometria testicular, ao índice gonadossomático, à proporção volumétrica dos constituintes do parênquima testicular, ao diâmetro tubular, à altura do epitélio seminífero e ao comprimento dos túbulos seminíferos. Abordam-se ainda as diferenças entres as razões celulares que permitem conclusões sobre o rendimento do processo espermatogênico e índices de células de Sértoli das três espécies.

  11. Mapping trends of large and medium size carnivores of conservation interest in Romania

    Directory of Open Access Journals (Sweden)

    Mihai Cristian Adamescu

    2014-07-01

    Full Text Available We analysed yearly estimates of population size data during 2001-2012 for five carnivores species of conservation interest (Ursus arctos, Canis lupus, Lynx lynx, Felis silvestris and Canis aureus. Population size estimations were done by the game management authorities and integrated by the competent authorities on the Ministry of Environment and Climate Change. Trends in data were detected using non-parametric Mann-Kendall test. This test was chosen considering the short length of data series and its usefulness for non-normal distributed data. The trend was tested at three spatial scales: game management units (n=1565, biogeographical region (n=5 and national. Trends depicted for each game management unit were plotted using ArcGIS, resulting species trend distribution maps. For the studied period increasing population trends were observed for Ursus arctos, Canis lupus, Canis aureus and Lynx lynx, while for Felis silvestris there was no trend recorded. Such an analysis in especially useful for conservation proposes, game management and reporting obligations under article 17 of the EC Habitat Directive, using population trend as a proxy for population dynamics. We conclude that the status of the five carnivore species is favourable during the study period.

  12. Changes in Mediterranean mesocarnivore communities along urban and ex-urban gradients

    Institute of Scientific and Technical Information of China (English)

    Mariano R RECIO; Carmen M ARIJA; Sara CABEZAS-DAZ; Emilio VIRGS

    2015-01-01

    Urbanization causes wildlife habitat loss, fragmentation, and the replacement of specialist species by generalists and/or exotic taxa. Because mesocarnivores are particularly vulnerable to habitat modifications, the rapid expansion of urban areas and the increasing trend for ex-urban development occurring in Mediterranean ecosystems may be major drivers of change in mesocarnivore communities. We combined camera trapping and sign surveys to quantify the richness and relative abundance of a set of wild and domestic mesocarnivores. We quantified these variables controlling for the gradient of urbanism, ex-urbanism, and other environmental variables in patches of natural vegetation in the region of Madrid (central Spain), and a non-urbanized control area ~220 km south of Madrid city. Using conditional autoregressive models (CAR) and model selection procedures, we found that urbanization influenced mesocarnivore community composition but this influence was not detrimental for all the species tested. Generalist carnivores such as the red foxVulpes vulpes were more abundant in urban and ex-urban areas. Ex-urban development creates overlapping areas between wild and domestic species (such as the domestic catFelis catus and the wildcat Felis silvestris) but contact between wild and domestic carnivores in natural areas is unlikely. Detection of species in the control area was very low. Therefore, the impact of urbanization in causing changes in mesocarnivore communities may be less than other factors such as illegal predator culling [Current Zoology 61 (5): 793–801, 2015].

  13. Molecular identification of Cryptosporidium spp. in seagulls, pigeons, dogs, and cats in Thailand

    Directory of Open Access Journals (Sweden)

    Koompapong Khuanchai

    2014-01-01

    Full Text Available Zoonotic Cryptosporidium spp., particularly C. meleagridis, C. canis, and C. felis, are enteric protozoa responsible for major public health concerns around the world. To determine the spread of this parasite in Thailand, we conducted molecular identification of Cryptosporidium spp. from animal samples around the country, by collecting and investigating the feces of seagulls (Chroicocephalus brunnicephalus and Chroicocephalus ridibundus, domestic pigeons (Columba livia domestica, dogs, and cats. Seagull and pigeon samples were collected at the seaside and on the riverside to evaluate their potential for waterborne transmission. Ten pigeon samples were combined into one set, and a total of seven sets were collected. Seventy seagull samples were combined into one set, and a total of 13 sets were collected. In addition, 111 dog samples were collected from cattle farms, and 95 dog and 80 cat samples were collected from a temple. We identified C. meleagridis in pigeons, Cryptosporidium avian genotype III in seagulls, C. canis in dogs, and C. felis in cats. In the temple, the prevalence was 2.1% (2/95 for dogs and 2.5% (2/80 for cats. No Cryptosporidium was found in dog samples from cattle farms. These are the first findings of C. meleagridis in domestic pigeons, and Cryptosporidium avian genotype III in seagulls. Our study invites further molecular epidemiological investigations of Cryptosporidium in these animals and their environment to evaluate the public health risk in Thailand.

  14. Seroprevalence of antibodies to Rickettsia typhi in the Waikato region of New Zealand.

    Science.gov (United States)

    Lim, M Y; Weinstein, P; Bell, A; Hambling, T; Tompkins, D M; Slaney, D

    2016-08-01

    The first reported New Zealand-acquired case of murine typhus occurred near Auckland in 1989. Since then, 72 locally acquired cases have been recorded from northern New Zealand. By 2008, on the basis of the timing and distribution of cases, it appeared that murine typhus was escalating and spreading southwards. To explore the presence of Rickettsia typhi in the Waikato region, we conducted a seroprevalence study, using indirect immunofluorescence, Western blot, and cross-adsorption assays of blood donor samples. Of 950 human sera from Waikato, 12 (1·3%) had R. typhi antibodies. The seroprevalence for R. typhi was slightly higher in northern Waikato (1·4%) compared to the south (1·2%; no significant difference, χ 2 P = 0·768 at P < 0·05). Our results extend the reported southern range of R. typhi by 140 km and indicate it is endemic in Waikato. Evidence of past Rickettsia felis infections was also detected in six sera. Globally, R. felis is an emerging disease of concern and this pathogen should also be considered when locally acquired rickettsiosis is suspected. If public health interventions are to be implemented to reduce the risk of rickettsioses as a significant public health problem, improvements in rickettsial diagnostics and surveillance will be necessary. PMID:27040715

  15. N-acetylcysteine, a novel treatment for Helicobacter pylori infection.

    Science.gov (United States)

    Huynh, Hien Quoc; Couper, Richard T L; Tran, Cuong D; Moore, Lynette; Kelso, Richard; Butler, Ross N

    2004-01-01

    N-Acetylcysteine (NAC), being both a mucolytic agent and a thiol-containing antioxidant, may affect the establishment and maintenance of H. pylori infection within the gastric mucus layer and mucosa. Agar and broth dilution susceptibility tests determined the MIC of H. pylori strain SSI to NAC. H. pylori load in SSI strain-infected C57BL mice was determined as colony forming units per gram of gastric tissue. Gastritis assessment was scored and gastric surface hydrophobicity was determined by contact angle measurement. MICs of NAC were 5 to 10 and 10 to 15 mg/ml using the agar dilution and broth dilution methods, respectively. NAC (120 mg per day for 14 days) reduced the H. pylori load in mice by almost 1 log compared with sham treatment. Pretreatment with NAC (40 mg/day) also significantly reduced the H. pylori load but did not prevent H. pylori colonization. Both H. pylori infection and NAC reduced the surface hydrophobicity of murine gastric mucosa. No significant differences were observed in the gastritis scores of H. felis- or H. pylori-infected mice receiving either NAC or sham treatments. This study demonstrates that NAC inhibits the growth of H. pylori in both agar and broth susceptibility tests and in H. pylori-infected mice. NAC did not alter the severity of H. pylori- or H. felis-induced gastritis. PMID:15628716

  16. Chlamydial plasmids and bacteriophages.

    Science.gov (United States)

    Pawlikowska-Warych, Małgorzata; Śliwa-Dominiak, Joanna; Deptuła, Wiesław

    2015-01-01

    Chlamydia are absolute pathogens of humans and animals; despite being rather well recognised, they are still open for discovery. One such discovery is the occurrence of extrachromosomal carriers of genetic information. In prokaryotes, such carriers include plasmids and bacteriophages, which are present only among some Chlamydia species. Plasmids were found exclusively in Chlamydia (C.) trachomatis, C. psittaci, C. pneumoniae, C. suis, C. felis, C. muridarum and C. caviae. In prokaryotic organisms, plasmids usually code for genes that facilitate survival of the bacteria in the environment (although they are not essential). In chlamydia, their role has not been definitely recognised, apart from the fact that they participate in the synthesis of glycogen and encode proteins responsible for their virulence. Furthermore, in C. suis it was evidenced that the plasmid is integrated in a genomic island and contains the tetracycline-resistance gene. Bacteriophages specific for chlamydia (chlamydiaphages) were detected only in six species: C. psittaci, C. abortus, C. felis, C. caviae C. pecorum and C. pneumoniae. These chlamydiaphages cause inhibition of the developmental cycle, and delay transformation of reticulate bodies (RBs) into elementary bodies (EBs), thus reducing the possibility of infecting other cells in time. Plasmids and bacteriophages can be used in the diagnostics of chlamydioses; although especially in the case of plasmids, they are already used for detection of chlamydial infections. In addition, bacteriophages could be used as therapeutic agents to replace antibiotics, potentially addressing the problem of increasing antibiotic-resistance among chlamydia.

  17. Contribution to the knowledge of helminthofauna of wild mammals of Somalia.

    Science.gov (United States)

    Iori, A; Lanfranchi, P

    1996-12-01

    Within a survey of parasitic infections in wild mammals of Somalia, during the first semester of 1983 and the second of 1984, endoparasites were collected from 11 host species: Gazella soemmeringi, Gazella spekei, Madoqua saltiana, Phacochoerus aethiopicus, Xerus rutilus, Lepus sp., Genetta genetta, Herpestes (Galerella) sanguineus, Felis sylvestris libyca, Felis caracal, Canis mesomelas. A total of 22 species of helminths (20 nematode and 2 metacestode species) were identified. Lepus sp. is a new host for Heteroxinema (P.) proboscidiphora and Herpestes sanguineus for Oxinema sp. Teladorsagia hamata had not been previously reported in G. spekei; the genital cone and the corresponding supporting apparatus of this nematode are described. Two metacestodes were collected from the mesenteric membranes of a P. aethiopicus. The shape, number and length of their rostellar hooks, the size of the larvocysts, the occurrence of pseudostrobilum, are similar to those of the Taenia regis metacestode: but the size and shape of the hooks of the Somalian warthog larvocysts are slightly different and they have not been recovered from the musculature as previously reported in the intermediate hosts of T. regis. PMID:9257339

  18. Parásitos en perros de San Juan Bautista, Isla Robinson Crusoe, Chile Parasites in dogs from San Juan Bautista, Robinson Crusoe Island, Chile

    Directory of Open Access Journals (Sweden)

    D González-Acuña

    2008-01-01

    Full Text Available Se estudió la fauna parasitaria de 40 perros en el poblado de San Juan Bautista, Isla Robinson Crusoe, Chile. El 50% (n = 20 de los perros presentó huevos de tipo Ancylostomideos, Strongyloideos y/o de especies Isospora sp. y Cystoisospora canis. No se encontraron muestras positivas a cestodos. El 100% de los perros presentó alguno de los siphonapteros Ctenocephalides canis, C. felis y/o Pulex irritans. En un perro se aisló un ejemplar de la garrapata café del perro Rhipicephalus sanguineus (2,5%. Se discute la importancia de los presentes resultados.The parasitological fauna of 40 dogs was studied in San Juan Bautista, Robinson Crusoe Island, Chile. 50% (n = 20 of the dogs had eggs of the Ancylostomid, Strongylid type and/or the oocysts of Isospora sp. and Cystoisospora canis. No positive samples of cestodes were found. 100% of the dogs were parasited by the fleas Ctenocephalides canis, C. felis and/or Pulex irritans. One brown dog tick, Rhipicephalus sanguineus, was found in one dog (2.5%. The importance of these findings is discussed.

  19. Phylogeography of the Neotropical catfish Pimelodus albicans (Siluriformes: Pimelodidae from río de la Plata basin, South America, and conservation remarks

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    Julia Vergara

    2008-03-01

    Full Text Available Pimelodus albicans Valenciennes, 1840 (common name "moncholo" or "bagre blanco" is an endemic species of the family Pimelodidae in the río de la Plata basin. Phylogenetic approach based on cytochrome b sequences was performed to test the existence of a unique evolutionary lineage in P. albicans and to discriminate populations units or subpopulations related to a migration behavior of this taxon in the río de la Plata basin. This study included 34 samples of P. albicans of different collecting sites in the río de la Plata estuary and in the río Arrecifes belonging to the río Paraná basin. Among 614 base pairs in the cytochrome b sequence data set, 203 were variable and 120 were phylogenetically informative sites in P. albicans. A total of twenty haplotypes, nucleotide diversity (p = 0.032 and haplotype diversity = 0.941 were found. Tajima's test showed significant value D= -1.88 (pPimelodus albicans Valenciennes, 1840 (popularmente conhecida como moncholo ou bagre branco é uma espécie endêmica da família Pimelodidae na bacia do rio da Prata. Estudos filogeográficos baseados nas seqüências do citocromo b mitocondrial foram realizados para testar a existência de uma única linhagem evolutiva in P. albicans e para discriminar unidades populacionais relacionadas ao comportamento migratório desse táxon na bacia do rio da Prata. Um total de 34 amostras de P. albicans provenientes de diferentes lugares de coleta no estuário do rio da Prata e rio Arrecifes na bacia do rio Paraná foram analisados. Entre as 614 pares de bases do citocromo b no conjunto de dados, 203 deles variaram e 120 foram sítios filogeneticamente informativos para P. albicans. No presente estudo foi encontrado um total de vinte haplótipos, diversidade de nucleotídeos (p = 0,032 e diversidade de haplótipos = 0,941. O teste de Tajima mostrou valores significativos D= -1,88 (p<0,05 rejeitando a hipótese de mutação neutra para os dados de P. albicans. Todas as an

  20. Ciclo reprodutivo de Parauchenipterus striatulus (Pisces - Auchenipteridae na represa de Ribeirão das Lajes - RJ

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    Araújo F.G.

    2000-01-01

    Full Text Available Este trabalho teve como objetivo avaliar o ciclo reprodutivo do bagre de água doce Parauchenipterus striatulus, na represa de Ribeirão das Lajes, Rio de Janeiro. Foram descritos aspectos morfológicos dos estádios de maturação gonadal bem como variações no índice gonadossomático, relação peso:comprimento, fator de condição e condição gonadal. A amostragem dos peixes foi feita bimestralmente entre abril de 1996 e maio de 1997 com o uso de redes de espera de 100m de comprimento, 4m de altura e com malha entre 25 e 65mm de distância entrenós. Os 339 indivíduos capturados ao longo do período de amostragem corresponderam a 40% do total de peixes. Cinco estádios de maturação gonadal foram determinados por meio de análise macroscópica para ambos os sexos: imaturo, maturação I, maturação II, maduro, desovado (fêmeas e esvaziado (machos. O índice gonadossomático foi mais elevado entre outubro e março para ambos os sexos, sugerindo amplo período de desova enquanto o fator de condição atingiu seu máximo entre fevereiro e junho, mostrando uma relação inversa entre essas duas características. A relação peso-comprimento para machos foi de W= 0,0095L3,0862 e fêmeas de W= 0,0116L3,0126, não tendo sido encontradas diferenças significativas entre os coeficientes de alometria para ambos os sexos.

  1. A preliminary inventory of the catfishes of the lower Rio Nhamundá, Brazil (Ostariophysi, Siluriformes

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    Rupert Collins

    2015-04-01

    O Rio Nhamundá é um rio de água clara, pouco conhecido, que drena parte do Escudo das Guianas em território brasileiro. Nesse estudo, nós reportamos os resultados de um levantamento ictiofaunístico preliminar dessa área, tendo como foco os bagres (Siluriformes. Nós identificamos um total de 36 espécies (31 gêneros, sete famílias provenientes de nossa coleta, e adicionamos 11 espécies já conhecidas para o rio. De maneira geral, os resultados de nossa pesquisa mostram que mesmo levantamentos rápidos podem gerar informações importantes sobre a biodiversidade de peixes amazônicos, sugerindo potenciais espécies novas, ampliando a área de distribuição de espécies, além de apontar a necessidade de revisões taxonômicas e estudos genéticos para alguns taxa. Para além das formas tradicionais de dados coletados em pesquisas de biodiversidade (i.e. espécimes preservados, nosso estudo fornece "novas" formas de dados, como DNA barcodes e imagens com o padrão de coloração dos espécimes vivos, informações essas que serão de valor inestimável para futuros estudos que abordem grupos taxonômicos difíceis.

  2. Otocinclus cocama, a new uniquely colored loricariid catfish from Peru (Teleostei: Siluriformes, with comments on the impact of taxonomic revisions to the discovery of new taxa

    Directory of Open Access Journals (Sweden)

    Roberto E. Reis

    2004-09-01

    Full Text Available A new, uniquely colored species of the loricariid catfish genus Otocinclus, O. cocama is described from a tributary to the lower río Ucayali in northern Peru. The new species is distinguished from other Otocinclus species by two putatively autapomorphic features, the distinct color pattern, consisting of vertically elongated blotches spanning from the dorsal midline to the ventral border of flanks, and by a complete lateral line. The phylogenetic relationships of the new species are investigated and it is apparently more closely related to a clade formed by O. huaorani, O. bororo, O. mariae, and O. mura. Comments on the impact of taxonomic revisions for the discovery and description of previously undetected biodiversity are also presented.Uma nova espécie de bagre loricariídeo de coloração única do gênero Otocinclus, O. cocama, é descrita de um afluente do baixo río Ucayali no norte do Peru. A nova espécie se distingue das demais espécies de Otocinclus por dois caracteres supostamente autopomórficos, o padrão de coloração diferenciado, que consiste em marcas alongadas verticalmente desde a linha média dorsal até a porção ventral dos flancos, e por uma linha lateral completa. As relações filogenéticas da nova espécie são investigadas e ela é aparentemente mais proximamente relacionada ao clado formado por O. huaorani, O. bororo, O. mariae e O. mura. Ao final, são apresentados comentários sobre o impacto de revisões taxonômicas no descobrimento e descrição de biodiversidade previamente não detectada.

  3. Evolution of opercle bone shape along a macrohabitat gradient: species identification using mtDNA and geometric morphometric analyses in neotropical sea catfishes (Ariidae).

    Science.gov (United States)

    Stange, Madlen; Aguirre-Fernández, Gabriel; Cooke, Richard G; Barros, Tito; Salzburger, Walter; Sánchez-Villagra, Marcelo R

    2016-08-01

    Transitions between the marine and freshwater macrohabitat have occurred repeatedly in the evolution of teleost fishes. For example, ariid catfishes have moved from freshwater to marine environments, and vice versa. Opercles, a skeletal feature that has been shown to change during such transitions, were subjected to 2D geometric morphometric analyses in order to investigate evolutionary shape changes during habitat transition in ariid catfishes and to test the influence of habitat on shape changes. A mtDNA marker, which proved useful in previous studies, was used to verify species identities. It greatly improved the assignment of specimens to a species, which are difficult to assign by morphology alone. The application of a mtDNA marker confirmed the occurrence of Notarius biffi in Central America, South of El Salvador. Molecular identification together with principal component analysis (PCA) and further morphological inspection of neurocrania indicated the existence of a cryptic species within Bagre pinnimaculatus. Principal component (PC) scores of individual specimens clustered in morphospace by genus rather than by habitat. Strong phylogenetic structure was detected using a permutation test of PC scores of species means on a phylogenetic tree. Calculation of Pagel's λ suggested that opercle shape evolved according to a Brownian model of evolution. Yet canonical variate analysis (CVA) conducted on the habitat groups showed significant differences in opercle shapes among freshwater and marine species. Overall, opercle shape in tropical American Ariidae appears to be phylogenetically constrained. This verifies the application of opercle shape as a taxonomic tool for species identification in fossil ariid catfishes. At the same time, adaptation to freshwater habitats shows characteristic opercle shape trajectories in ariid catfishes, which might be used to detect habitat preferences in fossils. PMID:27547357

  4. Broad histopathologic patterns of non-glabrous skin and glabrous skin from patients with a new variant of endemic pemphigus foliaceus-part 1.

    Science.gov (United States)

    Howard, Michael S; Yepes, Maria M; Maldonado-Estrada, Juan G; Villa-Robles, Edinson; Jaramillo, Andrés; Botero, Jorge H; Patiño, Pablo J; Hashimoto, Takashi; Abreu-Velez, Ana M

    2010-02-01

    A prospective, controlled epidemiologic survey performed in El Bagre, Colombia revealed a new variant of endemic pemphigus disease, occurring in a gold mining region. The disease resembled Senear-Usher syndrome, and occurred in an endemic fashion. The aim of this study is to describe the most frequent histopathologic patterns in non-glabrous skin and in glabrous skin observed in these patients, and their clinical correlation. The study was performed on non-glabrous skin biopsies of 30 patients from the dominantly clinical affected areas (either on the chest, arms or face). Simultaneously, biopsies from the palms were obtained in 10 randomly chosen patients of the 30 total patients. The specimens were examined following hematoxylin and eosin (H&E) staining. The most common blisters observed were subcorneal, although in some cases intraspinous and subepidermal blisters were visualized. Our results showed a very heterogeneous pattern of histopathologic patterns in non-glabrous skin, which seemed to correlate with the clinical features. The most common pattern was typical pemphigus foliaceus-like, with some lupus erythematosus-like features. A non-specific, chronic dermatitis pattern prevailed in the clinically controlled patients taking daily corticosteroids. In the patients who have had the most severe and relapsing pemphigus, early sclerodermatous changes and scleredermoid alterations prevailed in their reticular dermis. In addition to the scleredermoid alterations, the reticular dermis showed a paucity of appendageal structures. On the contrary, in the palms, a similar pattern was seen in all cases, including thickening of the stratum corneum, hypergranulosis, edema in the papillary and reticular dermis and a dermal perivascular lymphocytic infiltrate. The direct immunofluorescence of the glabrous vs. the non-glabrous skin also showed some differences. We conclude that the histopathologic features of this new variant of endemic pemphigus are complex, therefore

  5. Detection of mercury and other undetermined materials in skin biopsies of endemic pemphigus foliaceus.

    Science.gov (United States)

    Abréu Vélez, Ana María; Warfvinge, Gunnar; Herrera, Walter Leon; Abréu Vélez, Clara Eugenia; Montoya M, Fernando; Hardy, David M; Bollag, Wendy B; Hashimoto, Ken

    2003-10-01

    A novel variant of endemic pemphigus foliaceus (EPF) was described among individuals in an area surrounding El Bagre, Colombia, South America. The population in this rural mining community is exposed to high environmental levels of mercury, used for gold extraction, as well as other minerals, metalloids, and trace elements (e.g., quartz, rutile, granite, magnetite, and almenite) and ultraviolet radiation. Fifty control subjects and fifty EPF patients in the endemic area were examined for the presence of mercury in skin biopsies and hair, using autometallographic and mass spectroscopic analyses, respectively. Simultaneously, serum levels of IgE were measured, and cutaneous tests for hypersensitivity reactions were performed. Using autometallography, mercuric sulfides/selenides were detected in 14 of 51 skin biopsies distributed similarly in the control and patient groups. However, significantly higher serum IgE levels and mercury concentrations in hair, urine, and nails were found in patients compared with controls. Microscopic analysis revealed mercuric sulfides/selenides concentrated within and around the sweat gland epithelium, as well as in dendritic cells. Five skin biopsies from EPF patients and five from controls that tested positive for the presence of mercuric sulfides/selenides by autometallography were randomly selected for electron microscopic analysis. This analysis revealed a mixed electron-dense and electron-light material closely associated with desmosomes in patients. However, there were intracellular vesicles containing an amalgam of electron-dense and electron-light materials only in the EPF patients. Thus, EPF-affected individuals are exposed to high levels of environmental mercuric sulfides/selenides and other elements. This is the first study reporting mercuric sulfides/selenides in skin biopsies from people living in a focus of EPF, and these compounds may play a role in the pathogenesis of autoimmunity. PMID:14501287

  6. Therapeutic efficacy test in malaria falciparum in Antioquia, Colombia

    Directory of Open Access Journals (Sweden)

    Álvarez Tania

    2006-02-01

    Full Text Available Abstract Objective Evaluate the frequency of failure of eight treatments for non-complicated malaria caused by Plasmodium falciparum in patients from Turbo (Urabá region, El Bagre and Zaragoza (Bajo Cauca region, applying the 1998 protocol of the World Health Organization (WHO. Monotherapies using chloroquine (CQ, amodiaquine (AQ, mefloquine (MQ and sulphadoxine-pyrimethamine (SP, and combinations using chloroquine-sulphadoxine-pyrimethamine (CQ-SP, amodiaquine-sulphadoxine-pyrimethamine (AQ-SP, mefloquine-sulphadoxine-pyrimethamine (MQ-SP and artesunate-sulphadoxine-pyrimethamine (AS-SP, were examined. Methodology A balanced experimental design with eight groups. Samples were selected based on statistical and epidemiological criteria. Patients were followed for 21 to 28 days, including seven or eight parasitological and clinical evaluations, with an active search for defaulting patients. A non-blinded evaluation of the antimalarial treatment response (early failure, late failure, adequate response was performed. Results Initially, the loss of patients to follow-up was higher than 40%, but the immediate active search for the cases and the monetary help for transportation expenses of patients, reduced the loss to 6%. The treatment failure was: CQ 82%, AQ 30%, MQ 4%, SP 24%, CQ-SP 17%, AQ-SP 2%, MQ-S-P 0%, AS-SP 3%. Conclusion The characteristics of an optimal epidemiological monitoring system of antimalarial treatment response in Colombia are discussed. It is proposed to focus this on early failure detection, by applying a screening test every two to three years, based on a seven to 14-day follow-up. Clinical and parasitological assessment would be carried out by a general physician and a field microscopist from the local hospital, with active measures to search for defaulter patients at follow-up.

  7. Harttia merevari, a new species of catfish (Siluriformes: Loricariidae from Venezuela

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    Francisco Provenzano R.

    2005-12-01

    Full Text Available Harttia merevari, a new species of loricariid catfish, is described from eight specimens captured in the upper Caura River, Orinoco River basin, Venezuela. The new species is recognized by the following combination of characters: abdomen naked; two or three preanal plates; a bony plate before each branchial opening; seven lateral plates between the pectoral and pelvic fins; maxillary barbel short and attached to the oral disk by a fleshy fold; head dorsal surface and anterior portion of the body light or dark yellow with numerous, round black spots; posterior region of the body light or dark yellow with five black transverse bands, dorsal central area of the two anterior bands diffused. The discovery of this new species extends the distribution of the genus northwest to include the Orinoco River basin on the northern slope of the Guyana shield.Harttia merevari, uma espécie nova de bagre loricarideo é descrita com base em oito exemplares capturados no alto rio Caura, bacia do rio Orinoco, Venezuela. A espécie nova se distingue pela seguinte combinação de caracteres: abdome nu; duas ou três placas preanais; presença de uma placa óssea anterior a cada abertura branquial; sete placas laterais entre as nadadeiras peitorais e pélvicas; barbilhão maxilar curto e unido ao disco oral por uma dobra carnosa; superfície dorsal da cabeça e região anterior do corpo amarelo escuro ou marrom claro com numerosos pontos negros arredondados, região dorsal e posterior do corpo amarelo escuro ou marrom claro com cinco bandas transversais negras, as duas bandas anteriores com a parte dorsocentral difusa. A descoberta desta espécie nova estende a distribuição do gênero ao noroeste incluindo a bacia do rio Orinoco na vertente norte do Escudo das Guianas.

  8. Prevalência da infecção pelo Toxoplasma gondii em animais domésticos, silvestres e grupamentos humanos da Amazônia

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    José João Ferraroni

    1980-01-01

    Full Text Available Através de Reação de Hemaglutinação Indireta para toxoplasmose foram examinadas amostras de sangue de dez diferentes espécies de animais domésticos e silvestres, de um grupamento humano da cidade de Manaus-Amazonas e de um grupamento humano indígena de área distante, no território de Roraima. Em 108 animais domésticos, o exame sorológico foi reagente em 90,6% dos gatos (Felis catus, 68,4% dos cães (Canis familiaris, 60,0% dos bovinos (Bos sp, 41,2% dos galináceos (Gallus sp e 40,0% dos palmípedes (Cairina sp. Nos 104 animais silvestres foram reagentes 75,0% dos felídeos (Felis sp, 63,6% dos marsupiais (Didelphis marsupialis e Marmosa sp, 63,3% dos primatas (Saimiri sp e 61,1% dos roedores (Proechimys. Entre os dois grupos humanos a prevalência foi de 70,6% nos 51 habitantes da área de Manaus, 64,8% nos 37 silvícolas de Roraima. Os autores discutem os resultados obtidos, assim como os diversos aspectos envolvidos na epidemiologia da toxoplasmose e chamam a atenção para a existência de mecanismos de transmissão ainda não esclarecidos, enfatizando a necessidade de maiores estudos dessa zoonose.Serological examination for Toxoplasma gondii in human blood samples and in blood samples from ten different species of animals obtained in Manaus, State of Amazonas-Brazil, are compared with results obtained from similar examinations of blood from humans living in other areas of the Amazon basin. The domestic cat (Felis catus showed the highest levels of antibody for Toxoplasma gondii, whereas the domestic chicken (Gallus domesticus and duck (Cairina sp the lowest. The other animals showed similar intermediate levels of antibody to this protozoa. The authors discuss the results and several aspects of the involvement in epidemiology of toxoplasmosis and call attention to some transmission mechanisms not yet elucidated.

  9. Comparative study of renal sodium transport between ouabain-hypertensive rats and ouabain-nonhypertensive rats

    Institute of Scientific and Technical Information of China (English)

    GE Heng; Lü Zhuo-ren

    2006-01-01

    Objective: To compare renal sodium transport, using fractional excretions of lithium(FEii)as a marker of proximal tubule sodium reabsorption, between hypertensive and non-hypertensive ouabaintreated rats and further to elucidate the role of ouabain in pathogenesis of hypertension. Methods:Thirty male Sprague-Dawley rats weighting 180-200 g were randomly divided into normal control group and ouabain treated group. Rats were infused with 1 ml/kg · d normal saline or 27.8 μg/kg · d ouabain intraperitoneally once a day respectively. Systolic blood pressure (SBP), heart rate and body weight were recorded weekly. Rats were sacrificed 6 weeks after treatment. Blood and 24-hour urine sample were collected to measure the serum and urinary concentration of sodium, trace lithium and creatinine. Endogenous creatinine clearance rate (Ccr), fractional excretions of sodium (FENa), fractional excretions of lithium (FELi) and fractional reabsorption of sodium in the postproximal tubules (FDRNa) were calculated.Ouabain levels of plasma and renal tissue, plasma renin activity, angiotensin Ⅱ and aldosterone concentration were determined. Results: 65% of the ouabain-treated rats achieved significantly higher SBP after 4weeks, compared with that of the saline control groups or self baseline (P<0. 01). But in the other 35%of the ouabain-treated rats, their SBP was similar with control group during the experiment (P>0. 05).The body weight, heart rate and food intake between the 3 groups were no significant differences (P>0.05). FELi and FDRNa were significantly lower in ouabain-hypertensive group compared with ouabain-nonhypertensive group and control group(P<0.01 and P<0.05). The FELi and FDRNa of ouabain-nonhypertensive groups were similar with control group(P>0.05). Ccr and FENa were comparable between the 3 groups (P>0. 05). Plasma and renal tissue ouabain levels, plasma renin activity, angiotensin Ⅱ and aldosterone contents in ouabain-hypertensive rats were

  10. 从全球案例看移动支付的关键点

    Institute of Scientific and Technical Information of China (English)

    2015-01-01

    全球移动支付最活跃的国家不是美国,而是非洲肯亚。M-PESA是一个20 07年推出的移动支付服务,有超过1 900万注册用户,占国家人口70%,每天有超过600万笔交易在平台上完成。日本可以说是移动支付领头羊,早于2004年就由NTT Docomo推出第一台Feli Ca支付手机,比苹果Apple Pay早了10年。在2011年已经有超过33%日本人使用过手机进行支付,同时间欧美只有不到15%。

  11. Frequency of parasites and Salmonella infection in captive maned-wolf, Chrysocyon brachyurus, kept in Zoos at the State of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Gilioli R.

    2000-01-01

    Full Text Available Thirty-one captive maned wolves (Chrysocyon brachyurus, Illiger 1815 from 11 Zoos at the State of São Paulo, Brazil, were screened to investigate the presence of parasites and Salmonella infection by parasitological diagnostic methods and fecal selective culture. The most frequent ecto and endoparasites found were Ctenocephalides felis (56.2%, Rhipicephalus sanguineus (12.5%, Ancylostoma caninum (45.1%, Strongyloides sp. (29.0%, Uncinaria stenocephala (3.2%, Capillaria sp. (3.2%, Entamoeba sp. (22.9%, Sarcocystis sp. (29.0%, Cryptosporidium sp. (19.3%, Eimeria sp. (19.3%, Giardia sp. (9.6% and Isospora sp. (3.2%. Four different serotypes of Salmonella were identified in six animals (25%. Only one infected animal showed clinical signs of diarrhea. The ability to harbor Salmonella spp. as normal nonpathogenic bacteria of the gastrointestinal tract may be a physiological adaptation of this specie.

  12. Transcriptome analysis of feline infectious peritonitis virus infection.

    Science.gov (United States)

    Mehrbod, Parvaneh; Harun, Mohammad Syamsul Reza; Shuid, Ahmad Naqib; Omar, Abdul Rahman

    2015-01-01

    Feline infectious peritonitis (FIP) is a lethal systemic disease caused by FIP virus (FIPV). There are no effective vaccines or treatment available, and the virus virulence determinants and pathogenesis are not fully understood. Here, we describe the sequencing of RNA extracted from Crandell Rees Feline Kidney (CRFK) cells infected with FIPV using the Illumina next-generation sequencing approach. Bioinformatics analysis, based on Felis catus 2X annotated shotgun reference genome, using CLC bio Genome Workbench is used to map both control and infected cells. Kal's Z test statistical analysis is used to analyze the differentially expressed genes from the infected CRFK cells. In addition, RT-qPCR analysis is used for further transcriptional profiling of selected genes in infected CRFK cells and Peripheral Blood Mononuclear Cells (PBMCs) from healthy and FIP-diagnosed cats.

  13. PREVALENCE OF ANTIBODIES TO SELECTED VIRUSES AND PARASITES IN INTRODUCED AND ENDEMIC CARNIVORES IN WESTERN MADAGASCAR.

    Science.gov (United States)

    Pomerantz, Julie; Rasambainarivo, Fidisoa T; Dollar, Luke; Rahajanirina, Leon Pierrot; Andrianaivoarivelo, Radosoa; Parker, Patricia; Dubovi, Edward

    2016-07-01

    Introduced animals impact endemic populations through predation, competition, and disease transmission. Populations of endemic carnivores in Madagascar are declining, and pathogens transmitted from introduced species may further endanger these unique species. We assessed the exposure of introduced and endemic carnivores to common viral and parasitic pathogens in two national parks of Madagascar (Kirindy Mitea National Park and Ankarafantsika National Park) and their neighboring villages. We also identified variables associated with the presence of antibodies to these pathogens in fosa ( Cryptoprocta ferox ). Introduced and endemic species were exposed to canine parvovirus, canine herpesvirus, feline calicivirus, and Toxoplasma gondii . Domestic dogs ( Canis familiaris ) and cats ( Felis catus ) may be sources of infection for these pathogens. Prevalence of antibodies to Toxoplasma in captured fosa was >93%, and adults were more likely to be exposed than immature individuals. Our data provide a basis upon which to evaluate and manage risks of pathogen transmission between species. PMID:27195685

  14. Rickettsial pathogens and arthropod vectors of medical and veterinary significance on Kwajalein Atoll and Wake Island

    Directory of Open Access Journals (Sweden)

    Durden, L.

    2012-05-01

    Full Text Available Modern surveys of ectoparasites and potential vector-borne pathogens in the Republic of the Marshall Islands and Wake Island are poorly documented. We report on field surveys of ectoparasites from 2010 with collections from dogs, cats, and rats. Five ectoparasites were identified: the cat flea Ctenocephalides felis, a sucking louse Hoplopleura pacifica, the mites Laelaps nuttalli and Radfordia ensifera, and the brown dog tickRhipicephalus sanguineus. Ectoparasites were screened for rickettsial pathogens. DNA from Anaplasma platys, a Coxiella symbiont of Rhipicephalus sanguineus, anda Rickettsia sp. were identified by PCR and DNA sequencing from ticks and fleas on Kwajalein Atoll. An unidentified spotted fever group Rickettsia was detected in a pool of Laelaps nuttalli and Hoplopleura pacifica from Wake Island. The records of Hoplopleura pacifica, Laelaps nuttalli, and Radfordia ensifera and the pathogens are new for Kwajalein Atoll and Wake Island.

  15. A hypothesis linking sodium and lithium reabsorption in the distal nephron

    DEFF Research Database (Denmark)

    Thomsen, Klaus; Shirley, DG

    2006-01-01

      A hypothesis is proposed linking Na(+) and Li(+) reabsorption in the distal nephron. The handling of these two ions in the distal nephron is related because they share the same apical membrane entry mechanism: the amiloride-sensitive Na(+) channel (ENaC). However, the two ions exit the cell......(+) entrance across the apical membrane in those segments in which ENaC is active. Method. We propose a model that incorporates this concept. RESULTS: The model indicates that the lowering of FE(Li dist) during dietary Na(+) restriction can be explained by activation of apical ENaC in extra sub...... of the apical membrane in ENaC-containing sub-segments, which is known to occur in this condition. CONCLUSION: We conclude that the model may improve current understanding of both Na(+) and Li(+) handling in the distal nephron...

  16. Atypical Toxoplasma gondii genotype in feral cats from the Fernando de Noronha Island, northeastern Brazil.

    Science.gov (United States)

    Melo, R P B; Almeida, J C; Lima, D C V; Pedrosa, C M; Magalhães, F J R; Alcântara, A M; Barros, L D; Vieira, R F C; Garcia, J L; Mota, R A

    2016-07-15

    Toxoplasma gondii isolates from Brazil have a different phenotypic and genotypic pattern, with predominance of virulent isolates and recombinant genotypes, compared to the North Hemisphere. Considering that a new T. gondii genotype, non-pathogenic to mice, was previously identified from free-range chickens from the Fernando de Noronha Island, Brazil, this study aimed to identify genotypes of this parasite in tissue samples of feral cats (Felis catus) from this Brazilian Island. Anti-T. gondii IgG antibodies were detected in 18/31 (58%) feral cats. Two non-virulent T. gondii isolates were obtained by mouse bioassay. Genotyping was performed by PCR-RFLP using 10 genetic markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, PK1, L358 and Apico) and an atypical strain of T. gondii (ToxoDB #146) was identified. This is the first report of this genotype in feral cats. PMID:27270396

  17. 装备零讯

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    日海上自卫队第十艘亲潮级潜艇服役;英国发射“天网”5A军事通信卫星;巴海军“阿戈斯塔”90B潜艇将安装新型推进装置;南非海军第二艘209/1400级常规潜艇服役;法军将有27个步兵团配装FELIN未来士兵系统;美国海军SSN775核攻击潜艇展开后期试航试验;印第一艘核潜艇计划于下半年下水

  18. Evidence of feline immunodeficiency virus, feline leukemia virus, and Toxoplasma gondii in feral cats on Mauna Kea, Hawaii

    Science.gov (United States)

    Danner, R.M.; Goltz, Dan M.; Hess, S.C.; Banko, P.C.

    2007-01-01

    We determined prevalence to feline immunodeficiency virus (FIV) antibodies, feline leukemia virus (FeLV) antigen, and Toxoplasma gondii antibodies in feral cats (Felis catus) on Mauna Kea Hawaii from April 2002 to May 2004. Six of 68 (8.8%) and 11 of 68 (16.2%) cats were antibody positive to FIV and antigen positive for FeLV, respectively; 25 of 67 (37.3%) cats were seropositive to T. gondii. Antibodies to FeLV and T. gondii occurred in all age and sex classes, but FIV occurred only in adult males. Evidence of current or previous infections with two of these infectious agents was detected in eight of 64 cats (12.5%). Despite exposure to these infectious agents, feral cats remain abundant throughout the Hawaiian Islands. ?? Wildlife Disease Association 2007.

  19. Feeding profile of Mepraia spinolai, a sylvatic vector of Chagas disease in Chile.

    Science.gov (United States)

    Chacón, F; Bacigalupo, A; Quiroga, J F; Ferreira, A; Cattan, P E; Ramírez-Toloza, G

    2016-10-01

    American trypanosomiasis is a chronic disease transmitted mainly by vectors. The hematophagous triatomine vectors transmit Trypanosoma cruzi to a wide variety of mammals, which usually are their food source. This study determined the feeding profile of Mepraia spinolai, a sylvatic triatomine vector, present in endemic areas of Chile. Vectors were captured in the north-central area of Chile. Samples of intestinal contents were analyzed by an Enzyme-linked immunosorbent assay (ELISA) that identifies and discriminates the presence of serum antigens from Homo sapiens and nine animal species (Canis familiaris, Felis catus, Capra hircus, Mus musculus, Gallus gallus, Octodon degus, Thylamys elegans, Phyllotis darwini and Oryctolagus cuniculus). Our data indicate the most frequent feeding source in this area was P. darwini, followed by O. degus, O. cuniculus, M. musculus, G. gallus, T. elegans, C. familiaris, F. catus and C. hircus. Mixed food sources were also identified. PMID:27349188

  20. Toxoplasmose em animais silvestres e domésticos da região de Botucatu, estado de São Paulo, Brasil

    Directory of Open Access Journals (Sweden)

    Ednir Salata

    1985-02-01

    Full Text Available Os Autores analisaram soros de 47 Cannis familiaris, de 9 Felis cattus, de 64 Didelphis marsupialis aurita, de 9 Dasypus novemcinctus, de 4 Cabassous tatouay e de 29 Rattus rattus, através da reação de imunofluorescência indireta, para pesquisar a presença de anticorpos anti Toxoplasma gondii. Estes foram encontrados apenas em C. familiaris (63,8% e em D. m. aurita (4,7%. Frente aos resultados obtidos, os Autores sugerem que novas pesquisas nesta área sejam realizadas, para que se conheça melhor a importância epidemiológica de várias espécies animais na disseminação da toxoplasmose.

  1. 鄂西南部棲霞灰岩煤系之幾種鱗木植物及其地層意義(節要)

    Institute of Scientific and Technical Information of China (English)

    斯行健

    1951-01-01

    @@ 1950 年冬田奇(王隹)先生寄來鱗木植物化石數塊,係採自宜都縣,松木坪之馬鞍山煤系.採集者爲周聖生黄鐘二氏.煤系層位在棲霞灰岩之下,而不整合覆於黄龍灰岩之上.植物化石經鑑定爲Sigillaria acutangula Halle,Lepidodendron oculus felis.(Abbado)Zeiller及Lep.ituense Sze sp.nov三種.此三種或可視作一種即Lep.oculus fells.詳細討論見於西文中.此種化石,在山西中部屬於上石盒子系.

  2. Caracterização da população de ectoparasitos em cães de núcleos de expansão urbana de Juiz de Fora, Minas Gerais, Brasil Characterization of ectoparasites on dogs in the nucleus of urban expansion of Juiz de Fora, Minas Gerais, Brazil

    Directory of Open Access Journals (Sweden)

    Denise F. Rodrigues

    2008-12-01

    Full Text Available No presente trabalho caracterizou-se a população de ectoparasitos em cães de núcleo de expansão urbana de Juiz de Fora, MG. As coletas foram realizadas de julho a setembro de 2003, examinando-se 101 cães SRD (sem raça definida. Os ectoparasitos foram coletados através de inspeção visual e tátil dos animais e acondicionados em frascos com etanol 70°GL e identificados sob estereoscopia. Espécimes foram clarificados e montados para análise em microscopia fotônica. Dentre os sifonápteros, Ctenocephalides felis foi mais prevalente (64,35% com intensidade média de 6,12 ± 5,37 sifonápteros/cão, seguido por Rhopalopsyllus lutzi (3,96%, híbrida C. felis X C. canis (1,98%, Tunga penetrans (1,98%. Aúnica espécie de fitiráptero encontrada foi Trichodectes canis (7,92% com 1,3 ± 0,51 fitiráptero/cão. Dentre os ixodídeos, Rhipicephalus sanguineus foi a espécie mais prevalente (49,50% com intensidade média de 6,44 ± 10,2 ixodídeos/ cão, seguido por Amblyomma cajennense (3,96%, Boophilus microplus (2,97%, A. ovale (1% e A. aureolatum (1%. As ninfas de ixodídeos foram separadas em ninfas de Amblyomminae (58,41% com 10,11 ± 10,09 ninfas/cão e ninfas Rhipicephalinae (24,75% com 2,64 ± 3,25 ninfas/cão. Em 3,96 % dos cães foram encontradas larvas de ixodídeo.The present work studied the characterization of ectoparasites on dogs in the nucleus of expansão urbana of Juiz de Fora, Minas Gerais, Brazil. The collections were conducted from July to September 2003, examining 101 dogs of no defined breed. The ectoparasites were collected through visual and tactile inspection of the animals' bodies, and were then placed in bottles with 70° GL ethanol and identified under a stereomicroscope. Specimens were clarified and mounted on slides for analysis under a photonic microscope. Among the siphonaptera, Ctenocephalides felis was the most prevalent (64.35%, with mean intensity of 6.12 ± 5.37 siphonaptera/dog, followed by Rhopalopsyllus

  3. Evidence of feline immunodeficiency virus, feline leukemia virus, and Toxoplasma gondii in feral cats on Mauna Kea, Hawaii.

    Science.gov (United States)

    Danner, Raymond M; Goltz, Daniel M; Hess, Steven C; Banko, Paul C

    2007-04-01

    We determined prevalence to feline immunodeficiency virus (FIV) antibodies, feline leukemia virus (FeLV) antigen, and Toxoplasma gondii antibodies in feral cats (Felis catus) on Mauna Kea Hawaii from April 2002 to May 2004. Six of 68 (8.8%) and 11 of 68 (16.2%) cats were antibody positive to FIV and antigen positive for FeLV, respectively; 25 of 67 (37.3%) cats were seropositive to T. gondii. Antibodies to FeLV and T. gondii occurred in all age and sex classes, but FIV occurred only in adult males. Evidence of current or previous infections with two of these infectious agents was detected in eight of 64 cats (12.5%). Despite exposure to these infectious agents, feral cats remain abundant throughout the Hawaiian Islands.

  4. Occupancy of the Invasive Feral Cat Varies with Habitat Complexity

    Science.gov (United States)

    Hohnen, Rosemary; Tuft, Katherine; McGregor, Hugh W.; Legge, Sarah; Radford, Ian J.; Johnson, Christopher N.

    2016-01-01

    The domestic cat (Felis catus) is an invasive exotic in many locations around the world and is thought to be a key factor driving recent mammal declines across northern Australia. Many mammal species native to this region now persist only in areas with high topographic complexity, provided by features such as gorges or escarpments. Do mammals persist in these habitats because cats occupy them less, or despite high cat occupancy? We show that occupancy of feral cats was lower in mammal-rich habitats of high topographic complexity. These results support the idea that predation pressure by feral cats is a factor contributing to the collapse of mammal communities across northern Australia. Managing impacts of feral cats is a global conservation challenge. Conservation actions such as choosing sites for small mammal reintroductions may be more successful if variation in cat occupancy with landscape features is taken into account. PMID:27655024

  5. Isolation of pathogenic yersiniae from wild animals in Bulgaria.

    Science.gov (United States)

    Nikolova, S; Tzvetkov, Y; Najdenski, H; Vesselinova, A

    2001-04-01

    Pathogenic Yersinia strains were isolated between December 1998 and April 1999 from 37 wild animals: rabbit (Lepus europeus), boar (Sus scrofa scrofa), asiatic jackal (Canis aureus), red fox (Vulpes vulpes), mouflon (Ovis musimon), european river otter (Lutra lutra), beech marten (Martes foina), polecat (Musleta putorius) and wild cat (Felis silvestris). It was established that among the wild animals Y. enterocolitica strains of serotype 0:3 predominated, accompanied by Y. pseudotuberculosis strains of serotype 0:3. In one sample from asiatic jackal and one sample from rabbit, Y. enterocolitica serotype 0:8 was isolated. Yersinia enterocolitica and Y. pseudotuberculosis strains were isolated from tonsils and tongues as well as from the viscera--lung, liver, heart, spleen, kidney and lymph nodes, mainly in young animals (1-2 years of age). The results showed that wild animals are a possible natural reservoir for pathogenic Y. enterocolitica and Y. pseudotuberculosis and are included in the epidemiological chain of yersinioses. PMID:11393816

  6. Helminths of foxes and other wild carnivores from rural areas in Greece.

    Science.gov (United States)

    Papdopoulos, H; Himonas, C; Papazahariadou, M; Antoniadou-Sotiriadou, K

    1997-09-01

    Twenty species of helminth parasites were identified from fox, wolf, jackal and wild cat material collected in Greece. Of the 314 foxes (Vulpes vulpes) examined, 18 helminth species were recovered comprising one trematode, eight cestodes, seven nematodes and two acanthocephalans, with the cestode species Mesocestoides sp. (73.2%), Joyeuxiella echinorhynchoides (24.5%) and the nematode species Uncinaria stenocephala (43.9%), and Toxara canis (28.6%) being the most prevalent. Five cestode and three nematode species were reported from six wolves (CaniS lupus), together with one trematode, three cestode and four nematode species from five jackals (Canis aureus) and two cestode and three nematode species from four wild cats (Felis silvestris) examined. The species J. echinorhynchoides, Taenia crassiceps and Onicola canis and the genera Spirometra, Rictularia and Pachysentis are reported here for the first time in Greece. The results are discussed in the light of the feeding characteristics of wild carnivores in rural areas of Greece. PMID:9705680

  7. On Some Issues of Human-Animal Studies: An Introduction.

    Science.gov (United States)

    Métraux, Alexandre

    2016-03-01

    Animals are "in" - since prehistoric times when humans (or their ancient ancestors) were hunting animals, and when they fabricated the Paleolithic dog as well as the Paleolithic cat. In less general terms, animals are "in" since they received names and were listed, observed, mummified, turned into totems, and, later on, dissected, tortured under laboratory conditions, trained as experimental subjects or "purified" as model organisms. And they are massively "in" again, but now from overtly legal and moral points of view, at least since the last two decades of the twentieth century. This is to say that modern members of the species Homo sapiens have always been connected to animals of the most various kinds - from the human flea (Pulex irritans) and the cat flea (Ctenocephalides felis) to marine mammals, such as dolphins and whales, from horses to parrots, from scallops to worms, and so on. PMID:26903370

  8. Rickettsia lusitaniae sp. nov. isolated from the soft tick Ornithodoros erraticus (Acarina: Argasidae).

    Science.gov (United States)

    Milhano, Natacha; Palma, Mariana; Marcili, Arlei; Núncio, Maria Sofia; de Carvalho, Isabel Lopes; de Sousa, Rita

    2014-05-01

    In this study a novel Rickettsia from the spotted fever group, isolated from Ornithodoros erraticus soft ticks collected from pigpens in the south of Portugal, is described. After initial screening revealed Rickettsia-positive ticks, isolation attempts were then performed. Successful isolates were achieved by shell-vial technique using Vero E6 cells at 28°C. Molecular characterization of the isolate was performed based on analysis of five rickettsial genes gltA, ompA, ompB, sca1 and htr with their subsequent concatenation along with other rickettsial species resulting in a clustering of the new isolate with Rickettsia felis and Rickettsia hoogstraalii. The degree of nucleotide sequence similarity with other rickettsiae fulfills the criteria for classification of our isolate as a novel species. The name Rickettsia lusitaniae sp. nov. (=CEVDI PoTiRo) is proposed for this new species found in O. erraticus.

  9. Molecular Detection of Fastidious and Common Bacteria as Well as Plasmodium spp. in Febrile and Afebrile Children in Franceville, Gabon.

    Science.gov (United States)

    Mourembou, Gaël; Fenollar, Florence; Socolovschi, Cristina; Lemamy, Guy Joseph; Nzoughe, Hermann; Kouna, Lady Charlene; Toure-Ndouo, Fousseyni; Million, Matthieu; Mbiguino, Angelique Ndjoyi; Lekana-Douki, Jean Bernard; Raoult, Didier

    2015-05-01

    Malaria was considered as the main cause of fever in Africa. However, with the roll back malaria initiative, the causes of fever in Africa may change. This study aimed to evaluate the prevalence of bacteria and Plasmodium spp. in febrile and afebrile (controls) children from Franceville, Gabon. About 793 blood samples from febrile children and 100 from controls were analyzed using polymerase chain reaction (PCR) coupled with sequencing. Plasmodium spp. was the microorganism most detected in febrile (74.5%, 591/793) and controls (13%, 13/100), P Gabon. Two fastidious bacteria, Rickettsia felis and Borrelia spp., were found. Inclusion of controls should improve the understanding of the causes of fever in sub-Saharan Africa.

  10. PREVALENCE OF ANTIBODIES TO SELECTED VIRUSES AND PARASITES IN INTRODUCED AND ENDEMIC CARNIVORES IN WESTERN MADAGASCAR.

    Science.gov (United States)

    Pomerantz, Julie; Rasambainarivo, Fidisoa T; Dollar, Luke; Rahajanirina, Leon Pierrot; Andrianaivoarivelo, Radosoa; Parker, Patricia; Dubovi, Edward

    2016-07-01

    Introduced animals impact endemic populations through predation, competition, and disease transmission. Populations of endemic carnivores in Madagascar are declining, and pathogens transmitted from introduced species may further endanger these unique species. We assessed the exposure of introduced and endemic carnivores to common viral and parasitic pathogens in two national parks of Madagascar (Kirindy Mitea National Park and Ankarafantsika National Park) and their neighboring villages. We also identified variables associated with the presence of antibodies to these pathogens in fosa ( Cryptoprocta ferox ). Introduced and endemic species were exposed to canine parvovirus, canine herpesvirus, feline calicivirus, and Toxoplasma gondii . Domestic dogs ( Canis familiaris ) and cats ( Felis catus ) may be sources of infection for these pathogens. Prevalence of antibodies to Toxoplasma in captured fosa was >93%, and adults were more likely to be exposed than immature individuals. Our data provide a basis upon which to evaluate and manage risks of pathogen transmission between species.

  11. Prevalence of antibodies to Sarcocystis neurona in cats from Virginia and Pennsylvania.

    Science.gov (United States)

    Hsu, Vasha; Grant, David C; Dubey, J P; Zajac, Anne M; Lindsay, David S

    2010-08-01

    Sarcocystis neurona is best known as the causative agent of equine protozoal myeloencephalitis of horses in the Americas. Domestic cats ( Felis domesticus ) were the first animals described as an intermediate host for S. neurona . However, S. neurona -associated encephalitis has also been reported in naturally infected cats in the United States. Thus, cats can be implicated in the life cycle of S. neurona as natural intermediate hosts. The present study examined the seroprevalence of IgG antibodies to merozoites of S. neurona in populations of domestic cats from Virginia and Pennsylvania. Overall, sera or plasma from 441 cats (Virginia = 232, Pennsylvania = 209) were tested by an indirect immunofluorescent assay at a 1ratio50 dilution. Antibodies to S. neurona were found in 32 (7%) of 441 cats. Of these, 22 (9%) of the 232 cats from Virginia and 10 (5%) of the 209 cats from Pennsylvania were seropositive for S. neurona . PMID:20476809

  12. Serological evidence of Coxiella burnetii exposure in native marsupials and introduced animals in Queensland, Australia.

    Science.gov (United States)

    Cooper, A; Goullet, M; Mitchell, J; Ketheesan, N; Govan, B

    2012-07-01

    The state of Queensland has the highest incidence of Q fever in Australia. In recent years, there has been an increase in human cases where no contacts with the typical reservoir animals or occupations were reported. The aim of this study was to determine the seroprevalence of Coxiella burnetii in Australian native animals and introduced animals in northern and southeastern Queensland. Australian native marsupials sampled included the brushtail possum (Trichosurus vulpecula) and common northern bandicoot (Isoodon macrourus). Introduced species sampled included dingoes (Canis lupus dingo), cats (Felis catus), foxes (Vulpes vulpes) and pigs (Sus scrofa). Serum samples were tested by ELISA for both phase II and phase I antigens of the organism using an Australian isolate. The serological evidence of C. burnetii infection demonstrated in these species has public health implications due to their increasing movement into residential areas in regional Queensland. This study is the first known investigation of C. burnetii seroprevalence in these species in northern Queensland. PMID:21892986

  13. On harmonic operation of Shanghai deep UV free electron laser

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    By choosing parameters in the modulator,the dispersive section and the seed laser,the spatial bunching of the electron beam can be correlated to then-th harmonic of ther adiator radiation,instead of the fundamental radiation in conventional high-gain harmonic generation(HGHG).Thus,the radiator undulator is operated at high harmonic mode.In this paper,the possibility of harmonic operation of Shanghai deep ultraviolet(SDUV)free electron laser (FEL)is studied.Discussions on the principle of harmonic operation,the simulation code development,the simulation results.and the proposed experimental procedure for verification of harmonic operation at the SDUV FEL are also presented.

  14. Trichinella infection in wildlife of the southwestern United States.

    Science.gov (United States)

    Pozio, E; Pence, D B; La Rosa, G; Casulli, A; Henke, S E

    2001-10-01

    Several potential mammalian reservoirs of sylvatic species of Trichinella were examined from Texas, New Mexico, and Arizona. During 1998-99, tongues were collected from a black bear (Ursus americanus) in Arizona; from 9 black bears, a coyote (Canis latrans), and a mountain lion (Felis concolor) in New Mexico; and from 154 coyotes, 32 raccoons (Procyon lotor), 13 opossums (Didelphis marsupialis), 4 ocelots (Leopardus pardalis), 3 bobcats (Lynx rufus), and 5 feral hogs (Sus scrofa) in southern Texas. Larvae of Trichinella murrelli were identified by a multiple-polymerase chain reaction analysis in 1 black bear (11.1%) from New Mexico and in 7 coyotes (4.5%) of Texas, whereas Trichinella spiralis larvae were detected in the black bear of Arizona. This is the first report of Trichinella infection in wildlife of New Mexico and Texas and extends the distribution of T. murrelli into the southwestern United States near the border of Mexico. PMID:11695403

  15. Aspectos Básicos del Manejo Médico de los Pequeños Felinos del Neotrópico

    OpenAIRE

    Nestor Varela

    2009-01-01

    Los félidos son un grupo diverso de carnívoros altamente especializados, su sistemática es aun debatida y controversial, por ejemplo los pequeños félidos antiguamente estaban agrupados bajo el género Felis, sin embargo, actualmente se han descrito cinco géneros y ocho especies presentes en América: Leopardus spp (3 especies), que compendia al ocelote (L. pardalis – peso promedio en kilogramos [pmKg] de 10,1); el margay (L. wiedii – pmKg 3,4); y la oncilla o tigrillo (L. tigrinus – pmKg 2,4); ...

  16. Rickettsiae in arthropods collected from the North African Hedgehog (Atelerix algirus) and the desert hedgehog (Paraechinus aethiopicus) in Algeria.

    Science.gov (United States)

    Khaldi, Mourad; Socolovschi, Cristina; Benyettou, Meryam; Barech, Ghania; Biche, Mohamed; Kernif, Tahar; Raoult, Didier; Parola, Philippe

    2012-03-01

    Hedgehogs have become a popular pet despite their potential role in zoonotic disease transmission. We conducted an entomological study in a mountainous region of northeast Algeria in which we collected 387 fleas (Archeopsylla erinacei) and 342 ticks (Rhipicephalus sanguineus and Haemaphysalis erinacei) from Paraechinus aethiopicus and Atelerix algirus hedgehogs. Of the hedgehogs sampled, 77.7% and 91% were infested with fleas and ticks, respectively. Significantly more ticks and fleas were collected from A. algirus than from P. aethiopicus. Rickettsia felis was detected in 95.5% of fleas and R. massiliae was detected in 6.25% of Rh. sanguineus ticks by molecular tools. A new Rickettsia species of the spotted fever group was detected in 11.25% of Rh. sanguineus and in 77% of H. erinacei ticks. Overall, we show that hedgehogs can act as hosts for ectoparasites infected with several rickettsial agents. These data justify a more detailed investigation of animal reservoirs for Rickettsiae. PMID:22222114

  17. Incidence of Old World screw-worm fly, Chrysomya bezziana in Iraq

    International Nuclear Information System (INIS)

    The Old World screw-worm fly (OWSWF), Chrysomya bezziana Villenuve, is a member of the insect family Calliphoridae and is an obligate parasite of warm-blooded animals in the tropics and sub-tropics (Norris and Murray 1964). Flies lay their eggs on the edge of wounds or body orifices; the resulting larvae invade the host tissues and produce lesions and infertility if the genitals become infested (Humphrey et al. 1980). Recorded hosts include cattle (Bos indicus), sheep (Ovis aries), goats (Caprus hircus), dogs (Canis familiaris), cats (Felis domesticus) and man (Homo sapiens) (Patton 1920, 1922, Stoddar and Peck 1962, Norris and Murray 1964). This investigation describes the incidence of myiasis caused by C. bezziana in Iraq from September 1996 to March 1998

  18. Small mammals of the Addo Elephant National Park

    Directory of Open Access Journals (Sweden)

    P. Swanepoel

    1975-07-01

    Full Text Available A survey of the small mammals of the Addo Elephant National Park resulted in a checklist, as well as information on relative numbers, distribution within the Park, reproductive activity, sex ratios, and body measurements. Forty mammals species occur in the Park, while three re-introduced species probably do not occur any longer. Of the 40 species 28 are considered small mammals comprising 13 rodent, eight carnivore, two shrew, two bat, one primate and one lagomorph species, as well as the aardvark: Crociduraflavescens, C. cyanea infumata, Rousettus aegyptiacus, Eptesicus capensis, Cercopithecus pygerythrus, Canis mesomelas, Ictonyx striatus, Poecilogale albinucha, Genetta sp., Herpestes pulverulentus, Suricata suricatta, Proteles cristatus, Felis caracal, Orycteropus afer, Lepus saxatilis, Cryptomys hottentotus, Hystrix africae-australis, Pedetes capensis, Graphiurus murinus, Aethomys namaquensis, Praomys natalensis, Rhabdomys pumilio, Mus minutoides, Rattus rattus, Saccostomys campestris, Desmodillus auricularis, Otomys irroratus and 0. unisulcatus.

  19. Dicty_cDB: Contig-U09287-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 14_1( DQ386114 |pid:none) Amaranthus tuberculatus biotype he... 59 3e-07 CP000471_27( CP000471 |pid:none) Ma...85... 59 3e-07 DQ386113_1( DQ386113 |pid:none) Amaranthus tuberculatus biotype he...none) Amaranthus tuberculatus biotype he... 56 3e-06 AP006861_136( AP006861 |pid:none) Chlamydophila felis F...rotoporphyrinogen ... 52 3e-05 DQ386118_1( DQ386118 |pid:none) Amaranthus tuberculatus biotype he... 52 4e-0...4 DQ394876_1( DQ394876 |pid:none) Amaranthus tuberculatus biotype AC... 49 5e-04 AE017221_230( AE017221 |pid

  20. Hepatozoon and Theileria species detected in ticks collected from mammals and snakes in Thailand.

    Science.gov (United States)

    Sumrandee, Chalao; Baimai, Visut; Trinachartvanit, Wachareeporn; Ahantarig, Arunee

    2015-04-01

    We report the detection of Hepatozoon and Theileria in 103 ticks from mammals and snakes in Thailand. By using a genus-specific 18S rRNA PCR, Hepatozoon and Theileria spp. were detected in 8% and 18%, respectively, of ticks (n=79) removed from mammals. Of the ticks removed from snakes (n=24), 96% were infected with Hepatozoon spp., but none were infected with Theileria. Phylogenetic analysis revealed that Hepatozoon spp. detected from Dermacentor astrosignatus and Dermacentor auratus ticks from Wild boar (Sus scrofa) formed a phylogenetic group with many isolates of Hepatozoon felis that were distantly related to a species group containing Hepatozoon canis and Hepatozoon americanum. In contrast, a phylogenetic analysis of the Hepatozoon sequences of snake ticks revealed that Hepatozoon spp. from Amblyomma varanense from King cobra (Ophiophagus hannah) and Amblyomma helvolum ticks from Indochinese rat snake (Ptyas korros), and Asiatic water snake (Xenochrophis piscator) are grouped with Hepatozoon spp. recently isolated from Monocellate cobras, Reticulated pythons and Burmese pythons, all of Thai origin, and with Hepatozoon sp. 774c that has been detected from a tick species obtained from Argus monitors in Australia. A phylogenetic analysis demonstrated that Theileria spp. from Rhipicephalus (Boophilus) microplus, Haemaphysalis obesa, and Haemaphysalis lagrangei ticks from Sambar deer (Cervus unicolor) cluster with the Theileria cervi isolates WU11 and 239, and Theileria sp. Iwate 141. We report for the first time a Hepatozoon species that shares genetic similarity with Hepatozoon felis found in Dermacentor astrosignatus and Dermacentor auratus ticks collected from Wild boars in Thailand. In addition, we found the presence of a Theileria cervi-like sp. which suggests the potential role of Haemaphysalis lagrangei as a Theileria vector in Thailand.

  1. A Study on Faunal Remains from the Yuxi Site%玉溪遗址动物骨骼初步研究

    Institute of Scientific and Technical Information of China (English)

    赵静芳; 袁东山

    2012-01-01

    Located in Three Gorges Area, this site is dated from middle and late Neolithic to Shangand Zhou period. The Number of Identified Specimens is 7755 at the site, which represents 28 species. They are Cervus unicolor, Muntiacus reeves, Bubalus bubalus, Sus domestica, Selenaretos thibetanus, Aretonyx eollaris, Canis familiaris, Felis bengalensis, Felis silvestris, Panthera tigris, Paguma larvata, Rhinoceros sp., Hystrix hodgsoni, Rhizomys sp., Macaca mulatta, Mylopharyngodon piceus, Cteno- pharyngodon ideUus, Silurus asotus, Acipenser sp., Chinemys reevesi, Trionychidae indet, AVES,and MOLLUSCA. Among these species, pigs and dogs are domesticated, while others are all wild animals. According to the statistic result of modification marks on animal bones, it is speculated that the process of dismembering animals and the procedure of manufacturing awl-shaped bone tools occurred at the site. The wild species suggest that the climate of the site might be subtropical at that time.%三峡地区的玉溪遗址跨越了新石器时代中晚期和商周时期,出土了丰富的动物骨骼。动物群包括28个种,其中猪、狗为家养动物,其余为野生动物。通过对动物骨骼上人工痕迹的观察统计,复原了肢解动物的程序以及“锥形器”的制作流程。野生动物则指示了遗址周围为亚热带气候。

  2. Anti-Rickettsia spp. antibodies in free-ranging and captive capybaras from southern Brazil

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    Fernanda S. Fortes

    2011-11-01

    Full Text Available Capybaras (Hydrochaeris hydrochaeris are among the main hosts of Amblyomma spp. ticks, which is able to transmit Rickettsia species to human beings and animals. Since they are often infested with potential vector ticks, capybaras may be used as sentinels for rickettsiosis, such as the Brazilian Spotted Fever. The aim of the present study was to determine the prevalence of antibodies against Rickettsia spp. using the indirect immunofluorescence assay (IFA in 21 free-ranging and 10 captive animals from the Zoological Park of the 'Bela Vista Biological Sanctuary' (BVBS, Itaipu Binational, Foz do Iguaçu, Southern Brazil. Antigens of six rickettsial species already identified in Brazil (Rickettsia rickettsii, R. parkeri, R. bellii, R. rhipicephali, R. amblyommii and R. felis were used for IFA. Ticks from each capybara were collected for posterior taxonomic identification. A total of 19 (61.3% samples reacted to at least one of tested species. Seropositivity was found in 14 (45.2%, 12 (38.7%, 5 (16.1%, 4 (12.9%, 3 (9.7% and 3 (9.7% animals for R. rickettsii, R. bellii, R. parkeri, R. amblyommii, R. felis and R. rhipicephali, respectively. Two captive capybaras presented suggestive titers of R. rickettsii infection and one sample showed homologous reaction to R. parkeri. Only one free-ranging capybara presented evidence R. bellii infection. Ticks collected on capybaras were identified as Amblyomma dubitatum e Amblyomma sp. Results evidenced the rickettsial circulation in the area, suggesting a potential role of capybaras on bacterial life cycle.

  3. Cystinuria Associated with Different SLC7A9 Gene Variants in the Cat

    Science.gov (United States)

    Raj, Karthik; Osborne, Carl; Giger, Urs

    2016-01-01

    Cystinuria is a classical inborn error of metabolism characterized by a selective proximal renal tubular defect affecting cystine, ornithine, lysine, and arginine (COLA) reabsorption, which can lead to uroliths and urinary obstruction. In humans, dogs and mice, cystinuria is caused by variants in one of two genes, SLC3A1 and SLC7A9, which encode the rBAT and bo,+AT subunits of the bo,+ basic amino acid transporter system, respectively. In this study, exons and flanking regions of the SLC3A1 and SLC7A9 genes were sequenced from genomic DNA of cats (Felis catus) with COLAuria and cystine calculi. Relative to the Felis catus-6.2 reference genome sequence, DNA sequences from these affected cats revealed 3 unique homozygous SLC7A9 missense variants: one in exon 5 (p.Asp236Asn) from a non-purpose-bred medium-haired cat, one in exon 7 (p.Val294Glu) in a Maine Coon and a Sphinx cat, and one in exon 10 (p.Thr392Met) from a non-purpose-bred long-haired cat. A genotyping assay subsequently identified another cystinuric domestic medium-haired cat that was homozygous for the variant originally identified in the purebred cats. These missense variants result in deleterious amino acid substitutions of highly conserved residues in the bo,+AT protein. A limited population survey supported that the variants found were likely causative. The remaining 2 sequenced domestic short-haired cats had a heterozygous variant at a splice donor site in intron 10 and a homozygous single nucleotide variant at a branchpoint in intron 11 of SLC7A9, respectively. This study identifies the first SLC7A9 variants causing feline cystinuria and reveals that, as in humans and dogs, this disease is genetically heterogeneous in cats. PMID:27404572

  4. Tiger, Bengal and Domestic Cat Embryos Produced by Homospecific and Interspecific Zona-Free Nuclear Transfer.

    Science.gov (United States)

    Moro, L N; Jarazo, J; Buemo, C; Hiriart, M I; Sestelo, A; Salamone, D F

    2015-10-01

    The aim of this study was to evaluate three different cloning strategies in the domestic cat (Felis silvestris) and to use the most efficient to generate wild felid embryos by interspecific cloning (iSCNT) using Bengal (a hybrid formed by the cross of Felis silvestris and Prionailurus bengalensis) and tiger (Panthera tigris) donor cells. In experiment 1, zona-free (ZP-free) cloning resulted in higher fusion and expanded blastocyst rates with respect to zona included cloning techniques that involved fusion or injection of the donor cell. In experiment 2, ZP-free iSCNT and embryo aggregation (2X) were assessed. Division velocity and blastocyst rates were increased by embryo aggregation in the three species. Despite fewer tiger embryos than Bengal and cat embryos reached the blastocyst stage, Tiger 2X group increased the percentage of blastocysts with respect to Tiger 1X group (3.2% vs 12.1%, respectively). Moreover, blastocyst cell number was almost duplicated in aggregated embryos with respect to non-aggregated ones within Bengal and tiger groups (278.3 ± 61.9 vs 516.8 ± 103.6 for Bengal 1X and Bengal 2X groups, respectively; 41 vs 220 ± 60 for Tiger 1X and Tiger 2X groups, respectively). OCT4 analysis also revealed that tiger blastocysts had higher proportion of OCT4-positive cells with respect to Bengal blastocysts and cat intracytoplasmic sperm injection blastocysts. In conclusion, ZP-free cloning has improved the quality of cat embryos with respect to the other cloning techniques evaluated and was successfully applied in iSCNT complemented with embryo aggregation. PMID:26332056

  5. What makes a feline fatal in Toxoplasma gondii's fatal feline attraction? Infected rats choose wild cats.

    Science.gov (United States)

    Kaushik, M; Knowles, S C L; Webster, J P

    2014-07-01

    Toxoplasma gondii is an indirectly transmitted protozoan parasite, of which members of the cat family (Felidae) are the only definitive hosts and small mammals such as rats serve as intermediate hosts. The innate aversion of rodents to cat odor provides an obstacle for the parasite against successful predation by the feline definitive host. Previous research has demonstrated that T. gondii appears to alter a rat's perception of the risk of being preyed upon by cats. Although uninfected rats display normal aversion to cat odor, infected rats show no avoidance and in some cases even show attraction to cat odor, which we originally termed the "Fatal Feline Attraction." In this study, we tested for the first time whether the "Fatal Feline Attraction" of T. gondii-infected rats differed according to the type of feline odor used, specifically whether it came from domestic cats (Felis silvestris catus) or wild cats-cheetahs (Acinonyx jubatus) or pumas (Felis concolor). In two-choice odor trials, where wild and domestic cat odors were competed against one another, consistent with previous findings we demonstrated that infected rats spent more time in feline odor zones compared with uninfected rats. However, we further demonstrated that all cat odors are not equal: infected rats had a stronger preference for wild cat odor over that of domestic cats, an effect that did not differ significantly according to the type of wild cat odor used (cheetah or puma). We discuss these results in terms of the potential mechanism of action and their implications for the current and evolutionary role of wild, in addition to domestic, cats in transmission of T. gondii. PMID:24907200

  6. Genetic relatedness versus biological compatibility between Aspergillus fumigatus and related species.

    Science.gov (United States)

    Sugui, Janyce A; Peterson, Stephen W; Figat, Abigail; Hansen, Bryan; Samson, Robert A; Mellado, Emilia; Cuenca-Estrella, Manuel; Kwon-Chung, Kyung J

    2014-10-01

    Aspergillus section Fumigati contains 12 clinically relevant species. Among these Aspergillus species, A. fumigatus is the most frequent agent of invasive aspergillosis, followed by A. lentulus and A. viridinutans. Genealogical concordance and mating experiments were performed to examine the relationship between phylogenetic distance and mating success in these three heterothallic species. Analyses of 19 isolates from section Fumigati revealed the presence of three previously unrecognized species within the broadly circumscribed species A. viridinutans. A single mating type was found in the new species Aspergillus pseudofelis and Aspergillus pseudoviridinutans, but in Aspergillus parafelis, both mating types were present. Reciprocal interspecific pairings of all species in the study showed that the only successful crosses occurred with the MAT1-2 isolates of both A. parafelis and A. pseudofelis. The MAT1-2 isolate of A. parafelis was fertile when paired with the MAT1-1 isolates of A. fumigatus, A. viridinutans, A. felis, A. pseudoviridinutans, and A. wyomingensis but was not fertile with the MAT1-1 isolate of A. lentulus. The MAT1-2 isolates of A. pseudofelis were fertile when paired with the MAT1-1 isolate of A. felis but not with any of the other species. The general infertility in the interspecies crossings suggests that genetically unrelated species are also biologically incompatible, with the MAT1-2 isolates of A. parafelis and A. pseudofelis being the exception. Our findings underscore the importance of genealogical concordance analysis for species circumscription, as well as for accurate species identification, since misidentification of morphologically similar pathogens with differences in innate drug resistance may be of grave consequences for disease management.

  7. ECTOFAUNA PARASITÁRIA DE CÃES URBANOS DOMICILIADOS ATENDIDOS EM CLÍNICAS VETERINÁRIAS PARTICULARES NA CIDADE DE LAVRAS, MG

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    Antonio Marcos Guimarães

    2011-03-01

    Full Text Available The objective of this study was to determine the frequency of ectoparasites in urban domiciled dogs treated at nine private veterinary clinics in the city of Lavras, Minas Gerais, Brazil. Visible and palpable ectoparasites specimens were collected manually from 67 dogs during the period of September 2004 to May 2005, kept in flasks with ethanol 70º GL and identified under a stereomicroscope. In the case of mites that cause mange, performed the analysis of the results obtained from 155 skin scrapings of the same number of dogs with clinical suspicion of parasitic dermatosis was performed, and sent to diagnostic at the Laboratory of Parasitic Diseases of Federal University of Lavras (UFLA, during the period of September 2002 to July 2007. A total of 540 ectoparasites were recorded, and four species were identified: Rhipicephalus sanguineus (60%, Ctenocephalides canis and C. felis (both 36%, and Dermatobia hominis larvae (4%. In scraped skin, positive results were verified in 12.9% (20/155, of which 80.0% with Demodex canis (16/20 and 20.0% with Sarcoptes scabiei var. canis (4/20. After ectoparasites identification a database with the dogs´medical records was made and analyzed by SPSS 12.0. It can be concluded that the predominant species of ectoparasites in dogs treated at private veterinary clinics in the city of Lavras, MG, were Ctenocephalides canis and C. felis (Siphonaptera: Pulicidae and R. sanguineus (Acari: Ixodidae and Demodex canis (Acari: Demodecidae that had a significant predominance (p<0.05 on scraped skin of young animals with suspicion of canine demodicosis.

  8. Gastric Helicobacter infection induces iron deficiency in the INS-GAS mouse.

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    Melanie J Thomson

    Full Text Available There is increasing evidence from clinical and population studies for a role of H. pylori infection in the aetiology of iron deficiency. Rodent models of Helicobacter infection are helpful for investigating any causal links and mechanisms of iron deficiency in the host. The aim of this study was to investigate the effects of gastric Helicobacter infection on iron deficiency and host iron metabolism/transport gene expression in hypergastrinemic INS-GAS mice. INS-GAS mice were infected with Helicobacter felis for 3, 6 and 9 months. At post mortem, blood was taken for assessment of iron status and gastric mucosa for pathology, immunohistology and analysis of gene expression. Chronic Helicobacter infection of INS- GAS mice resulted in decreased serum iron, transferrin saturation and hypoferritinemia and increased Total iron binding capacity (TIBC. Decreased serum iron concentrations were associated with a concomitant reduction in the number of parietal cells, strengthening the association between hypochlorhydria and gastric Helicobacter-induced iron deficiency. Infection with H. felis for nine months was associated with decreased gastric expression of iron metabolism regulators hepcidin, Bmp4 and Bmp6 but increased expression of Ferroportin 1, the iron efflux protein, iron absorption genes such as Divalent metal transporter 1, Transferrin receptor 1 and also Lcn2 a siderophore-binding protein. The INS-GAS mouse is therefore a useful model for studying Helicobacter-induced iron deficiency. Furthermore, the marked changes in expression of gastric iron transporters following Helicobacter infection may be relevant to the more rapid development of carcinogenesis in the Helicobacter infected INS-GAS model.

  9. Prevalence and genetic characteristics of Cryptosporidium, Enterocytozoon bieneusi and Giardia duodenalis in cats and dogs in Heilongjiang province, China.

    Science.gov (United States)

    Li, Wei; Li, Yijing; Song, Mingxin; Lu, Yixin; Yang, Jinping; Tao, Wei; Jiang, Yanxue; Wan, Qiang; Zhang, Siwen; Xiao, Lihua

    2015-03-15

    This study investigated 319 fecal specimens of cats (n=52) and dogs (n=267) from Heilongjiang province, China for the prevalence and genetic characteristics of Cryptosporidium, Enterocytozoon bieneusi, and Giardia duodenalis. PCR and DNA sequence analysis of the small subunit rRNA gene identified C. felis and C. parvum in one cat each (3.8%) and C. canis and C. ubiquitum in 6 dogs (2.2%). Polymorphisms in the ribosomal internal transcribed spacer and phylogenetic analysis characterized zoonotic E. bieneusi genotypes D, EbpC, NED1, and NED2 and host-adapted ones NED3, NED4, and PtEb IX in 18 dogs (6.7%) and human-pathogenic genotypes D and IV in 3 cats (5.8%). Genotyping based on the hypermutation of G. duodenalis triosephosphate isomerase gene (TPI) facilitated identification of assemblage F in a cat (1.9%) and assemblages C and E in 12 dogs (4.5%). Subtypes of G. duodenalis isolates were determined by measuring the diversity of both TPI nucleotide and amino acid sequences. C. canis, C. felis, C. parvum, E. bieneusi genotypes D, EbpC, and IV, and G. duodenalis assemblage C identified herein have been documented in human infections in China. C. canis, C. parvum, C. ubiquitum, and E. bieneusi genotypes D, EbpC, and IV carried by cats or dogs also existed in wastewater in China. The finding suggested pet animals could be reservoirs for human cryptosporidiosis, microsporidiosis, and giardiasis and potential sources of water contamination in China.

  10. Cystinuria Associated with Different SLC7A9 Gene Variants in the Cat.

    Science.gov (United States)

    Mizukami, Keijiro; Raj, Karthik; Osborne, Carl; Giger, Urs

    2016-01-01

    Cystinuria is a classical inborn error of metabolism characterized by a selective proximal renal tubular defect affecting cystine, ornithine, lysine, and arginine (COLA) reabsorption, which can lead to uroliths and urinary obstruction. In humans, dogs and mice, cystinuria is caused by variants in one of two genes, SLC3A1 and SLC7A9, which encode the rBAT and bo,+AT subunits of the bo,+ basic amino acid transporter system, respectively. In this study, exons and flanking regions of the SLC3A1 and SLC7A9 genes were sequenced from genomic DNA of cats (Felis catus) with COLAuria and cystine calculi. Relative to the Felis catus-6.2 reference genome sequence, DNA sequences from these affected cats revealed 3 unique homozygous SLC7A9 missense variants: one in exon 5 (p.Asp236Asn) from a non-purpose-bred medium-haired cat, one in exon 7 (p.Val294Glu) in a Maine Coon and a Sphinx cat, and one in exon 10 (p.Thr392Met) from a non-purpose-bred long-haired cat. A genotyping assay subsequently identified another cystinuric domestic medium-haired cat that was homozygous for the variant originally identified in the purebred cats. These missense variants result in deleterious amino acid substitutions of highly conserved residues in the bo,+AT protein. A limited population survey supported that the variants found were likely causative. The remaining 2 sequenced domestic short-haired cats had a heterozygous variant at a splice donor site in intron 10 and a homozygous single nucleotide variant at a branchpoint in intron 11 of SLC7A9, respectively. This study identifies the first SLC7A9 variants causing feline cystinuria and reveals that, as in humans and dogs, this disease is genetically heterogeneous in cats. PMID:27404572

  11. Molecular detection of bacterial and parasitic pathogens in hard ticks from Portugal.

    Science.gov (United States)

    Maia, Carla; Ferreira, Andreia; Nunes, Mónica; Vieira, Maria Luísa; Campino, Lenea; Cardoso, Luís

    2014-06-01

    Ticks are important vector arthropods of human and animal pathogens. As information about agents of disease circulating in vectors in Portugal is limited, the aim of the present study was to detect bacteria and parasites with veterinary and zoonotic importance in ticks collected from dogs, cats, and field vegetation. A total of 925 ticks, comprising 888 (96.0%) adults, 8 (0.9%) nymphs, and 29 (3.1%) larvae, were collected in 4 geographic areas (districts) of Portugal. Among those, 620 (67.0%) were removed from naturally infested dogs, 42 (4.5%) from cats, and 263 (28.4%) were questing ticks obtained from field vegetation. Rhipicephalus sanguineus was the predominant tick species, and the only one collected from dogs and vegetation, while all Ixodes ricinus specimens (n=6) were recovered from cats. Rickettsia massiliae and Rickettsia conorii were identified in 35 ticks collected from cats and dogs and in 3 ticks collected from dogs. Among ticks collected from cats or dogs, 4 Rh. sanguineus specimens were detected with Hepatozoon felis, 3 with Anaplasma platys, 2 with Hepatozoon canis, one with Anaplasma phagocytophilum, one with Babesia vogeli, one with Borrelia burgdorferi sensu lato and one with Cercopithifilaria spp. Rickettsia helvetica was detected in one I. ricinus tick collected from a cat. To the best of our knowledge, this was the first time that Cercopithifilaria spp., Ba. vogeli, H. canis, and H. felis have been detected in ticks from Portugal. The wide range of tick-borne pathogens identified, some of zoonotic concern, suggests a risk for the emergence of tick-borne diseases in domestic animals and humans in Portugal. Further studies on these and other tick-borne agents should be performed to better understand their epidemiological and clinical importance, and to support the implementation of effective control measures.

  12. Refinement of a commercial bench-top relaxin assay for pregnancy diagnosis using urine from domestic and nondomestic felids.

    Science.gov (United States)

    Harris, Laurie A; Steinetz, Bernard G; Bond, Jennifer B; Lasano, Sally; Swanson, William F

    2008-06-01

    Relaxin, a 6-kDa polypeptide hormone, is excreted in the urine during pregnancy in several mammalian species. A recent study showed that detection of urinary relaxin using a bench-top serum assay (Witness relaxin kit, Synbiotics Corp., San Diego, California 92127, USA) can be diagnostic for pregnancy in domestic cats (Felis silvestris catus), but it is unknown whether the bench-top kit is applicable with urine across felid species. Our objectives were to 1) examine modifications in urine processing to improve kit reliability in pregnant cats, 2) evaluate the impact of concentrating urine via filtration on relaxin detection, 3) assess the effect of sample freezing on relaxin concentrations, and 4) begin quantifying urinary relaxin levels in nondomestic felids. Urine and serum were collected from domestic cats and nondomestic cat species (Pallas' cat, Otocolobus manul; sand cat, Felis margarita; cheetah, Acinonyx jubatus; and lion, Panthera leo) at several times after breeding. Urine samples, subjected to various processing methods, were tested using the bench-top kit, and relaxin levels were later quantified via radioimmunoassay. For domestic cat urine samples, filtration and addition of protein/phosphate buffer improved the consistency of the relaxin kit for early pregnancy diagnosis. Urine freezing caused a slight (approximately 13%) but significant decrease in relaxin concentrations, but frozen-thawed samples still tested positive with the bench-top kit. In nondomestic felids, urinary relaxin immunoreactivity during pregnancy was similar to or higher than that of pregnant domestic cats, suggesting that relaxin is a reliable cross-species marker of pregnancy. Urinary relaxin was detectable using the bench-top kit in pregnant Pallas' cats, but urine samples from other species tested negative, regardless of processing methods. Findings suggest that measurement of urinary relaxin is a promising approach for noninvasive pregnancy diagnosis in exotic felids, but

  13. Directory of Open Access Journals (Sweden)

    Leandro Villa-Verde

    2013-03-01

    Full Text Available The genus Listrura, Glanapteryginae, currently comprises six valid species of small and elongated trichomycterid catfishes that inhabit coastal plains of Southern and Southeastern Brazil. As primarily fossorial dwellers, species of the genus display unusual morphological adaptations and restricted habitats, which make these fishes particularly susceptible to environmental disturbance. Listrura camposi is poorly known and ranked as a Critically Endangered catfish species, according to current Brazilian list of threatened species. Description of the species was based on a single specimen collected in 1940, and for seven decades no additional specimens were reported. As a consequence, basic information about the species is unavailable. Recently, a population of L. camposi was found inhabiting a small stream tributary to rio Ribeira de Iguape basin, São Paulo State, relatively close to the type locality. On the basis of newly collected material, the species is redescribed and the first data on its habitat and ecology are presented. Conservation status is also discussed.O gênero Listrura, Glanapteryginae, compreende atualmente seis espécies válidas de bagres pequenos e alongados que habitam as baixadas costeiras do Sul e Sudeste do Brasil. Principalmente de hábitos fossoriais, as espécies do gênero apresentam adaptações morfológicas incomuns e habitat restrito, o que torna estes peixes particularmente suscetíveis a distúrbios ambientais. Listrura camposi é pouco conhecida e considerada uma espécie Criticamente em Perigo, segundo as mais recentes listas brasileiras de espécies ameaçadas. A descrição da espécie foi baseada em um único exemplar coletado em 1940 e por sete décadas nenhum espécime adicional foi relatado. Como consequência, informações básicas sobre a espécie estão indisponíveis. Recentemente, uma população de L. camposi foi encontrada habitando um pequeno córrego tributário da bacia rio Ribeira de Iguape

  14. Sciades herzbergii oxidative stress biomarkers: an in situ study of an estuarine ecosystem (São Marcos' Bay, Maranhão, Brazil

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    Raimunda Nonata Fortes Carvalho-Neta

    2010-01-01

    Full Text Available In order to study the effects of environmental contamination on wild fish, sites were sampled in São Marcos' Bay. The first is located near the ALUMAR/ALCOA port, a potentially contaminated area. The second, located near the Coqueiro beach, was used as a reference area. The activity of antioxidant defence catalase (CAT and glutathione S-transferase (GST in S. herzbergii was compared with the biometric data and gonadosomatic index (GSI. The result showed that GSI decreased significantly in females (pO objetivo desse trabalho foi estudar os efeitos da contaminação ambiental em peixes amostrados em dois locais da Baía de São Marcos. O primeiro ponto está localizado próximo ao porto da ALUMAR/ALCOA, considerado como uma área potencialmente contaminada. O segundo ponto, situado na praia do Coqueiro, foi usado como uma área de referência. Dados da atividade da enzima de defesa antioxidante catalase (CAT e da glutationa S-transferase (GST em S. herzbergii foram comparados com os dados biométricos e o índice gonadossomático (GSI. Resultados mostraram que o GSI diminuiu significativamente em fêmeas (p <0.05 no local contaminado. A atividade da CAT foi mais alta nos peixes do local contaminado. Uma diferença significativa foi observada na atividade de GST de S. herzbergii no local contaminado e no local de referência (p <0.05. GSI possibilitou uma nova abordagem quanto à natureza da resposta de destoxificação nessa espécie de bagre porque este índice não apresentou correlação com as enzimas no local potencialmente contaminado, mas apresentou no local de referência. Assim, sugere-se que a boa correlação da GST/CAT e GSI poderia estar relacionada à reprodução dos animais no local de referência, mas não no local potencialmente contaminado. Se esse for o caso, pode-se concluir que GST/CAT e GSI podem ser utilizados como bons biomarcadores para avaliar contaminação aquática.

  15. Otocinclus batmani, a new species of hypoptopomatine catfish (Siluriformes: Loricariidae from Colombia and Peru

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    Pablo Lehmann A.

    2006-12-01

    Full Text Available A new species of the hypoptopomatine catfish genus Otocinclus is described from two localities in the upper Río Amazonas basin: a tributary of the Río Puré in Colombia and two tributaries to the Río Amazonas near Iquitos in Peru. The new taxon can be easily distinguished from all congeners, except Otocinclus cocama, by having a single, intensely pigmented, vertical W-shaped caudal fin spot and by having three discrete dark bands on dorsum, between the dorsal-fin base and the caudal fin. Otocinclus batmani differs from O. cocama by the absence of vertically elongated blotches from the dorsal midline to the ventral border of flanks, and by lacking a posterior extension of black pigmentation on the base of two central caudal-fin rays. Phylogenetic relationships of the new species are investigated and it is possibly more closely related to a clade formed by O. huaorani, O. mariae, O. bororo, O. mura, and O. cocama.Uma nova espécie de bagre hypoptopomatineo do gênero Otocinclus é descrita de duas localidades na bacia do alto rio Amazonas: um tributário do rio Puré na Colômbia e dois igarapés tributários do rio Amazonas próximo a Iquitos no Peru. O novo táxon pode ser facilmente distinguido dos demais congêneres, exceto Otocinclus cocama, por uma mancha vertical em forma de W, densamente pigmentada localizada na porção posterior da nadadeira caudal, e pela presença de três bandas discretas escuras no dorso, entre a base da nadadeira dorsal e a nadadeira caudal. De Otocinclus cocama ela é diferenciada pelo padrão de colorido composto de uma grande faixa escura lateral (vs. faixas verticais alongadas nos flancos e pela ausência de pigmentação negra prolongada na base dos dois raios centrais da nadadeira caudal. As relações filogenéticas da nova espécie são investigadas e ela é possivelmente mais proximamente relacionada a um clado formado por O. huaorani, O. mariae, O. bororo, O. mura e O. cocama.

  16. Environmental characterization of the reproductive season of migratory fish of the Sinú river (Córdoba, Colombia

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    Eduardo Kerguelén-Durango

    2015-09-01

    Full Text Available Objective. To characterize some abiotic factors during the reproductive season of migratory fish (bocachico Prochilodus magdalenae, dorada Brycon sinuensis, bagre blanco Sorubim cuspicaudus and barbul Pimelodus blochii in the Sinú River. Materials and methods. From April to October, 2008, in Carrizola (Tierralta, 36.31 km from the Urrá hydroelectric plant factors such as flow=Qriver, daily flow difference =ΔQriver, velocity=Vriver, temperature=Triver, electrical conductivity=CE, total dissolved solids=STD, turbidity=Turb, cloudiness=Nub, rainfall=Pluv and solar brightness=Bs were measured daily three times/day (07:00 h, 12:00 h, 17:00 h. Also, on the same schedule, ichthyoplankton was collected as an indicator of reproductive activity, assessed by reproductive frequency (Fr=number of days with presence of ichthyoplankton/number of days in the period evaluated X 100 and larval density (Dl=larval/m3. Results. The daily values of Qriver ranged between 278.7 and 838.5 m3/s, ΔQriver between 0.0 and 100.7 m3/s, Vriver between 1.40 and 1.53 m/s, Pluv between 0.0 and 88.2 mm, Triver between 27.0 and 28.7°C, STD between 73.0 and 302.3 mg/L, Turb between 9.7 and 679.7 NTU, CE between 81.0 and 361.7 mS/cm, Bs between 0.0 and 11.2 hours, Nub between 2.0 and 7.3 octaves. The Fr was 30.4%, with April (40.0% and May (74.2% as the higher activity reproductive months, while the Dl in the season was 4.9 larvae/m3, with the larvae group other species (2.9 larvae/m3 and bocachico (1.8 larvae/m3 as the most abundant. Conclusions. Most reproductive activity was recorded early in the season (April and May and parameters such as Qriver, Triver, Vriver, Pluv, STD and Nub may be considered final factors associated with migratory fish reproduction.

  17. Mercury contamination from artisanal gold mining in Antioquia, Colombia: The world's highest per capita mercury pollution.

    Science.gov (United States)

    Cordy, Paul; Veiga, Marcello M; Salih, Ibrahim; Al-Saadi, Sari; Console, Stephanie; Garcia, Oseas; Mesa, Luis Alberto; Velásquez-López, Patricio C; Roeser, Monika

    2011-12-01

    The artisanal gold mining sector in Colombia has 200,000 miners officially producing 30tonnes Au/a. In the Northeast of the Department of Antioquia, there are 17 mining towns and between 15,000 and 30,000 artisanal gold miners. Guerrillas and paramilitary activities in the rural areas of Antioquia pushed miners to bring their gold ores to the towns to be processed in Processing Centers or entables. These Centers operate in the urban areas amalgamating the whole ore, i.e. without previous concentration, and later burn gold amalgam without any filtering/condensing system. Based on mercury mass balance in 15 entables, 50% of the mercury added to small ball mills (cocos) is lost: 46% with tailings and 4% when amalgam is burned. In just 5 cities of Antioquia, with a total of 150,000 inhabitants: Segovia, Remedios, Zaragoza, El Bagre, and Nechí, there are 323 entables producing 10-20tonnes Au/a. Considering the average levels of mercury consumption estimated by mass balance and interviews of entables owners, the mercury consumed (and lost) in these 5 municipalities must be around 93tonnes/a. Urban air mercury levels range from 300ng Hg/m(3) (background) to 1million ng Hg/m(3) (inside gold shops) with 10,000ng Hg/m(3) being common in residential areas. The WHO limit for public exposure is 1000ng/m(3). The total mercury release/emissions to the Colombian environment can be as high as 150tonnes/a giving this country the shameful first position as the world's largest mercury polluter per capita from artisanal gold mining. One necessary government intervention is to cut the supply of mercury to the entables. In 2009, eleven companies in Colombia legally imported 130tonnes of metallic mercury, much of it flowing to artisanal gold mines. Entables must be removed from urban centers and technical assistance is badly needed to improve their technology and reduce emissions. PMID:22000915

  18. Induced spawning of the endangered Neotropical species Steindachneridion parahybae (Siluriformes: Pimelodidae

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    Danilo Caneppele

    2009-01-01

    Full Text Available The "surubim do Paraíba" (Steindachneridion parahybae is a freshwater catfish endemic to the Paraíba do Sul River basin, Brazil. This species has been seriously threatened by environmental disturbances in the last several decades. Wild Steindachneridion parahybae males and females were collected in 2003 and taken to the hatchery of a power plant of the Companhia Energética de São Paulo (CESP. Steindachneridion parahybae broodstocks were artificially induced to reproduce in December 2003 using a combination of carp pituitary extract (CPE and human chorionic gonadotropin (hCG. Oocytes and milt were stripped; the fertilized eggs were transferred to 60-liter conical incubators and hatched larvae distributed in nine horizontal trays. Exogenous feed was started just after yolk sac absorption. A high rate of cannibalism and photophobia were observed during the larval period, resulting in a 26% survival rate from larvae to fingerlings.O "surubim do Paraíba" (Steindachneridion parahybae é um bagre de água doce, endêmico da bacia do rio Paraíba do Sul, Brasil. Esta espécie foi seriamente ameaçada por distúrbios ambientais nas últimas décadas. Machos e fêmeas selvagens de Steindachneridion parahybae foram coletados em 2003 e transferidos para a piscicultura da CESP (Companhia Energética de São Paulo. Reprodutores de S. parahybae foram induzidos à reprodução artificial em dezembro de 2003 usando uma combinação de extrato hipofisário de carpa (CPE e gonadotropina coriônica humana (hCG. Após a extrusão dos óvulos e do sêmen, os ovos fertilizados foram transferidos para incubadoras cônicas de 60 litros e, em seguida, as larvas eclodidas distribuídas em nove incubadoras horizontais. Após a absorção do saco vitelino, a alimentação exógena foi iniciada. Uma alta taxa de canibalismo e fotofobia foram observados durante o período larval, resultando em uma taxa de sobrevivência de 26% de larvas para os alevinos

  19. Tissue-specific mercury concentrations in two catfish species from the Brazilian coast

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    Juliana de Souza Azevedo

    2012-06-01

    Full Text Available Some regions of Brazil have particularly high levels of mercury (Hg emissions due to industrial activities, and their fish species may therefore suffer high levels of contamination through bioaccumulation. In this paper we assess Hg contamination in the muscle, liver, gills and blood of two different species of catfish collected from the Cananéia and Santos-São Vicente estuaries, São Paulo State, Brazil. Fish from the most polluted site (Santos-São Vicente showed higher median Hg concentrations in both species (Cathorops spixii - liver: 1530 µg Kg-1, muscle: 327 µg Kg-1 and gill: 101 µg Kg-1; Genidens genidens - liver: 2617 µg Kg-1, muscle: 393 µg Kg-1 and gill: 118 µg Kg-1. Multivariate analysis revealed the importance and influence of key biological variables (size, condition, etc. in determining the overall level of Hg and its distribution within different tissues.Algumas regiões do Brasil apresentam, altos níveis de emissões de mercúrio (Hg devido às atividades industriais, e suas espécies de peixes podem, portanto, estar expostos a elevados níveis de contaminação por meio da bioacumulação. Neste trabalho avaliamos a contaminação por Hg no músculo, fígado, brânquias e sangue de duas espécies de bagres coletados nos estuários de Cananéia e Santos-São Vicente, São Paulo, Brasil. Peixes amostrados no local mais poluído (Santos-São Vicente mostraram maiores concentrações de Hg em ambas as espécies (Cathorops spixii - fígado: 1530 µg Kg-1, músculo: 327 µg Kg-1 e brânquias: 101 µg Kg-1; Genidens genidens - fígado: 2617 µg Kg-1, músculo: 393 µg Kg-1 e brânquias: 118 µg Kg-1. Análises multivariadas revelaram a importância e influência das principais variáveis biológicas (tamanho, condição, etc. no teor geral de Hg e sua distribuição nos diferentes tecidos.

  20. Flujos de energía en el sistema de surgencia tropical de la península de la Guajira , Caribe colombiano

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    García Camilo Bernardo

    2004-12-01

    Full Text Available La Guajira es un sistema de surgencia explotado en el Caribe colombiano. Con el propósito de integrar la información disponible sobre el sistema se construyó un modelo trófico de 27 grupos funcionales usando el programa ECOPATH 5.0 Beta. Se obtuvo una base cuantitativa a través de la construcción del modelo, para compararlo con otros sistemas de surgencia. La Guajira presenta una biomasa total de 68 t/km2/año, la producción primaria neta del sistema es de 1248,51 t/km2/año y las transferencias totales del sistema son de 3.275 t/km2/año, lo que
    indica un ecosistema con una surgencia moderada comparada con otros sitios. Las mayores transferencias de energía se dan del nivel trófico I-II (68,93%, aunque existe una importante proporción de flujos al detritus (32%. Las flotas pesqueras afectan principalmente los recursos
    de las fracciones del nivel trófico III, la flota artesanal esta ejerciendo mayor presión sobre el pámpano, bonito y jurel, la flota parguera sobre pargos y meros y la flota industrial sobre las corvinas. Los resultados en los atributos de madurez del sistema sugieren que La Guajira es un
    ecosistema inmaduro debido a que la relación P/R excede a uno y está en desarrollo debido a la baja ascendencia (33,7% y alta capacidad de desarrollo (66,3% como ocurre con otras surgencias donde se presentan valores de ascendencia entre 20% y 35%. Aunque los datos básicos de entrada en el modelo fueron buenos, debido a las investigaciones que se han elaborado entre 1995 y 2000, existen algunos grupos donde no se encuentra aun información disponible. Especialmente en los valores de biomasa del fitoplancton, invertebrados, bagres, peces depredadores
    pelágicos, así como estimaciones de producción en invertebrados, peces  depredadores pelágicos y peces pequeños pelágicos. No existe una cuantificación de las poblaciones de mamíferos y aves en el área de estudio, los cuales constituyen depredadores

  1. Nuclear abnormalities in erythrocytes and morphometric indexes in the catfish Cathorops spixii (Ariidae from different sites on the southeastern Brazilian coast

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    Juliana de Souza Azevedo

    2012-09-01

    Full Text Available Nuclear abnormalities in erythrocytes (NAE were taken as biomarkers in the catfish Cathorops spixii (Ariidae sampled in an estuary little affected by human activity (Cananéia and in three regions (Santos Channel: SC, Santos Bay: SB and São Vicente Channel: SVC of the Santos-São Vicente estuary impacted by various anthropogenic activities. Increases in NAE were observed in fish from SC and SVC sampled in the summer period as compared with specimens from the Cananéia estuary. These results suggest the presence of genotoxic compounds in these regions. However, the absence of significant differences in micronuclei frequency reflects slight mutagenic effects in these individuals. It is possible that the lower NAE frequency in specimens from SB might be associated with the greater remobilization and dilution of chemicals in this region. The low frequency of NAE in C. spixii from the Cananéia estuary is in accordance with the slight anthropogenic influence in this system, and may be suggestive of the absence of genotoxic and mutagenic effects in these organisms.Alterações nucleares eritrocitárias (ANE foram consideradas como biomarcadores nos bagres Cathorops spixii (Ariidae amostrados em um estuário com baixa influência humana (Cananéia e em três regiões (Canal de Santos: CS, Baía de Santos: BS e Canal de São Vicente: CSV no estuário de Santos-São Vicente, impactado por diversas atividades antropogênicas. Aumentos nas ANE foram observados nos peixes amostrados no CS e CSV durante o período de verão, quando comparados com os espécimes do estuário de Cananéia. Estes resultados sugerem a presença de compostos genotóxicos nessas regiões. Entretanto, ausência de diferenças significativas na freqüência de micronúcleos refletem baixos efeitos mutagênicos nesses indivíduos. As menores freqüências de ANE nos espécimes amostrados na BS podem estar associado à maior remobilização e diluição dos compostos químicos na regi

  2. The late Miocene Phractocephalus catfish (Siluriformes: Pimelodidae from Urumaco, Venezuela: additional specimens and reinterpretation as a distinct species

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    John G. Lundberg

    2003-12-01

    . However, the genus does not occur west or north of the Andes or Venezuelan coastal ranges. Recognizing the Urumaco Phractocephalus as a distinct species does not alter the obvious conclusion that this catfish marks a large river connection between the Caribbean coastal region and the Orinoco system during at least part of the Neogene. Other Urumaco fossils show this same biogeographic relationship.É descrita uma nova espécie fóssil de bagre pimelodídeo proveniente da Formação Urumaco do Mioceno superior no estado Falcón, Venezuela. O exemplar fóssil foi originalmente atribuído a espécie recente Phractocephalus hemioliopterus. Porém, com base em exemplares fósseis adicionais provenientes da Formação Urumaco, essa espécie extinta é descrita como uma nova espécie. As características diagnósticas da nova espécie †Phractocephalus nassi são as seguintes: 1 a metade posterior do frontal e a metade anterior do supraocciptal apresentam uma ornamentação caracterizada por altos relevos e sulcos alongados, assim como reticulações e algumas depressões subcirculares; 2 o mesetmóide é muito largo e está ornamentado; 3 a margem do etmóide lateral é convexa, não apresenta o entalhe orbital e não se projeta anteriormente sobre o côndilo palatino; 4 a fontanela cranial anterior está fechada e forma uma pequena depressão; 5 a margem do processo supraoccipital é redonda lateral e posteriormente, e cobre completamente o complexo de Weber quando visto dorsalmente; 6 o opérculo está ornamentado com reticulações e depressões; 7 o cleitro encontra-se densamente ornamentado ao longo da margem ventral da área de articulação do espinho peitoral; 8 o espinho peitoral encontra-se ornamentado com estrias e sulcos. †Phractocephalus nassi é comparada com a espécie recente P. hemioliopterus e com outra especie fóssil da Formação Solimões do Mioceno superior do Acre no Brasil, a qual ainda não foi descrita. Apresentam-se novas características diagn

  3. La malaria en Colombia, Antioquia y las zonas de Urabá y Bajo Cauca: panorama para interpretar la falla terapéutica antimalárica. Parte 1

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    Jaime Cardona Fonseca

    2003-03-01

    Full Text Available Antecedentes y Problema: en 1998-2002 se investigó en Antioquia (Turbo, Zaragoza y El Bagre, la eficacia terapéutica de medicamentos y combinaciones antimaláricos. La interpretación de la respuesta terapéutica (adecuada, fallas precoz y tardía requiere información adicional para una mejor comprensión. Objetivo: definir un marco de referencia para interpretar los resultados de las evaluaciones de respuesta terapéutica antimalárica, constituido por información sobre el comportamiento de los programas antimaláricos en Colombia y por información demográfica, epidemiológica, climatológica y socioeconómica sobre el departamento de Antioquia, las regiones de Urabá y Bajo Cauca y los municipios de Turbo y El Bagre. Metodología: se usó información oficial secundaria y en menor cantidad, información primaria. Con los datos recogidos se construyeron cuadros y gráficos, y se hicieron cálculos sobre otras variables. Resultados: se describe la malaria (paludismo en Colombia en 1960-2002. En 2002, de los 43,707 millones de habitantes, 22,403 millones (51,17% están expuestos (41,88% con bajo riesgo. El P. vivax es la especie predominante (66% de los casos, excepto en la región del Pacífico, donde el P. falciparum causa el 75% de los casos. Hay brotes de malaria cada 2 a 7 años; la más intensa epidemia en la historia conocida ocurrió en 1998. La mortalidad ha pasado de 25 por cien mil en 1983 a 3 por cien mil en 2000. El índice parasitario anual (IPA ha estado alrededor de 8 por mil expuestos después de 1990, valor que es el cuádruple del existente en 1960-1970. La ineficacia del programa ha sido evidente casi desde su inicio en 1958 y la tendencia de la enfermedad siempre ha sido creciente. El fracaso de los programas gubernamentales antimalaria no tiene origen en fenómenos principalmente biológicos (resistencias del plasmodio a los medicamentos y del anofeles a los insecticidas, ni tampoco en las “condiciones de orden p

  4. Live capture and handling of the European wildcat in central Italy

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    Lolita Bizzarri

    2010-08-01

    Full Text Available Abstract
    Between 2003 and 2006, a live-trapping of European wildcats (Felis silvestris was carried out in the Apennines (central Italy. Double-door tunnel cage traps were set along trap-lines. A box containing live quails as bait was securely attached to the side of each cage. Trapping was carried out in 8 sessions at a total of 60 trap-sites, mainly inside woods (65%. The distance between the traps ranged from 146 m to 907 m and the length of each trap-line ranged from 541 m to 2632 m. There were 16 captures of 11 different wildcats, the capture success rate being 1 wildcat/209 trap-days. Nine males and 2 females were caught, suggesting sex-biased trapping selection. In addition to wildcats, 20 non-target species were captured during the 8 sessions. No animal was injured by the traps and no wildcat was endangered by narcosis or handling. The technique proved to be effective for future field studies that envisage the radio-tracking of wildcats.

    Riassunto
    Cattura e immobilizzazione del gatto selvatico in Italia centrale.
    Tra il 2003 e il 2006 è stato svolto un programma di ricerca sul gatto selvatico europeo (Felis silvestris in un'area dell'Appennino centrale. Trappole a tunnel a doppio ingresso sono state distribuite lungo delle line-trap; a ciascun dispositivo è stato associato un box che ospitava esche vive (quaglie. Sono state effettuate 8 sessioni di trappolamento per un totale di 60 siti trappola, perlopiù in aree boscate (65%. La distanza tra le trappole variava da 146 m a 907 m mentre la lunghezza delle line-trap variava tra 541 m a 2632 m. I 16 eventi di cattura hanno permesso di marcare 11 individui diversi, 9 maschi e 2 femmine. La differenza del numero di catture tra maschi e femmine è probabilmente da attribuire ad una diversa selettività delle trappole. Durante le 8 sessioni, oltre al gatto selvatico, sono state catturate altre

  5. Central Tropical Pacific SST and Salinity Variability over the Little Ice Age

    Science.gov (United States)

    Sayani, H. R.; Cobb, K. M.; Khare, A.; Stone, C.; Grothe, P. R.; Chen, T.; Cheng, H.; Lu, Y.; Edwards, R. L.

    2015-12-01

    Anthropogenic trends are difficult to separate from natural variability in the tropical Pacific, where instrumental data coverage is sparse. Of particular interest are variations of climate in the pre-industrial era, but instrumental data from this time period is virtually nonexistent. Corals allow for the investigation of pre-industrial climate variability, but most living coral colonies rarely span more than a century. Fossil corals provide a valuable archive of climate variability over the past millennia [Cobb et al., 2013] and are best suited to the reconstruction of ENSO. For the reconstruction of mean climate, systematic offsets between proxy records from contemporaneous corals translates to large error bars on the resulting reconstructions [e.g. Felis et al., 2003; Pfeiffer et al., 2009]. By building composite paleoclimate records using multiple corals from the Line Islands (2°N - 6°N, 157°W - 162°W), we quantify mean climate state and climate variability in the central tropical Pacific (CTP) during the Little Ice Age (LIA). We compare paired d18O and Sr/Ca records from Line Island fossil corals to a large collection of modern coral d18O and Sr/Ca records from the same islands to estimate SST and salinity changes from the LIA to the late 20th century. Taken together, Sr/Ca records from three Palmyra Atoll fossil corals spanning 1630-1703CE suggest CTP temperatures may have been 1.7±0.9˚C cooler during much of the 17th century. Reconstructed seawater d18O values are indicative of drier conditions at Palmyra, consistent with a southward shift of the ITCZ during the LIA documented in sediments from the Line Islands [Sachs et al., 2009]. We compare the results from seven LIA-dated fossil corals from nearby Christmas Island (Kiritimati; 2˚N, 157˚W) to the results obtained from Palmyra, enabling us to resolve potential shifts in the meridional gradients of SST and hydrology in this region during the LIA. References:Cobb, K. M., et al. (2013) Science. doi

  6. Uso da técnica de Southern Blot/Hibridização associada à reação em cadeia da polimerase para aumentar a sensibilidade no diagnóstico das infecções por hemoplasmas em gatos domésticos: Use of Southern Blot/Hybridization technique associated to polymerase chain reaction to improve the sensitivity in the diagnosis of hemoplasma infections in domestic cats

    Directory of Open Access Journals (Sweden)

    Daniel B. Macieira

    2009-12-01

    Full Text Available O objetivo deste trabalho foi verificar se a técnica de Southern Blot/Hibridização (SB em associação à reação de polimerização em cadeia (PCR aumenta a sensibilidade na detecção de DNA de hemoplasmas em gatos domésticos (Felis catus. O sangue total foi coletado em tubos contendo o anticoagulante ácido etilenodiamino tetra-acético, o DNA extraído a partir de 149 animais e a PCR realizada com o uso de sequências iniciadoras espécie-específicas, para amplificar subunidade 16S do RNA ribossomal de Mycoplasma haemofelis e 'Candidatus M. haemominutum' dessas amostras. Para a hibridização, foram utilizadas sondas específicas quimicamente marcadas, e os resultados visualizados por meio da adição de substrato quimiluminescente seguida de autoradiografia. Dezoito (12,1% das 149 amostras testadas apresentaram resultado PCR-positivo para o DNA de hemoplasmas. A técnica de SB mostrou que 24/149 (16,1% amostras apresentaram resultado positivo para hemoplasmas, confirmando os 18 resultados PCR-positivos, além de revelar seis outros adicionais (p The aim of this study was to determine whether Southern Blot/Hybridization (SB associated to Polymerase Chain Reaction (PCR improves the sensitivity in the detection of hemoplasma DNA in domestic cats (Felis catus. Whole blood was collected in tubes containing the anticoagulant ethylenediamine tetra-acetic acid and DNA extracted from 149 animals. PCR was performed using species specific primers to amplify the 16S ribosomal RNA subunit of Mycoplasma haemofelis and 'Candidatus M. haemominutum' from these samples. Hybridization was performed using a 16S rDNA probes chemically labeled and the results were visualized using a chemiluminescent substrate addition followed by autoradiography. Eighteen (12.1% of the 149 tested samples had a positive PCR result for hemoplasma species DNA. SB/hybridization technique showed that 24/149 (16.1% samples were positive for hemoplasmas, confirming the 18 PCR

  7. Cortisol influence on testicular testosterone secretion in domestic cat: An in vitro study Ação do cortisol sobre a secreção de testosterona em testículos de gato doméstico: um estudo in vitro

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    Gelson Genaro

    2010-10-01

    Full Text Available The aim of the present experiment was to investigate the effect of corticosteroids (exogen on in vitro testosterone secretion after stress by transportation (40 minutes. Feline testes (Felis silvestris catus were incubated in the following media: TCM 199; TCM 199 + hCG 10_7M; TCM 199 + hydrocortisone 10_7M, or TCM 199 + hCG + hydrocortisone. The animals (n=21 were allocated into three groups: (S that arrived at 3 h prior to surgery, (A that remained in the laboratory for 36 h before being submitted to surgical procedure, and (C that were also allowed to remain for 36 hours in the laboratory before the surgical procedure, but whose testes had been incubated with hydrocortisone prior to incubation in the referred media. The results showed that group S secreted higher levels of testosterone, regardless of the culture media. It is noteworthy that the suppressing action of hydrocortisone sodium succinate led to a reduction in the testosterone concentration, despite the presence of hCG.O objetivo deste trabalho foi investigar o efeito da hidrocortisona sobre a secreção de testosterona após cultivo in vitro dos testículos, em distintas situações de estresse (transporte de gatos domésticos (Felis silvestris catus. Testículos foram incubados nos seguintes meios de cultura: TCM 199; TCM 199 + hCG 10_7M; TCM 199 + hydrocortisona 10_7M e TCM 199 + hCG + hidrocortisona. Os animais (n=21 foram alocados em 3 sub-grupos: (S animal admitido 3 horas antes da orquiectomia, (A animais orquiectomizados após 36 horas de permanência no biotério e (C animais que permaneceram por 36 horas no biotério antes da cirurgia e que tiveram seus testículos pré-incubados em hidrocortisona. Os resultados demonstraram que o grupo S secretou maiores valores de testosterona em todas as condições estudadas. É válido mencionar que a supressão promovida pela hidrocortisona também promoveu redução na concentração de testosterona no meio TCM 199, a despeito da

  8. Parasite meningomyelitis in cats in Uruguay Meningomielites parasitária em gatos no Uruguai

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    Rodolfo Rivero

    2011-09-01

    Full Text Available Two outbreaks of progressive hind limb paresis in cats (Felis catus caused by parasitic meningomyelitis in Uruguay are reported. The case studies occurred in 2008 and 2009 respectively, in the rural areas of Fray Bentos (33º 07' 40.39" S and were characterized by hindquarter paralysis. This paralysis was progressive and had a chronic progression of approximately 12 months until the death or euthanasia of the animals. Clinical symptoms started with ataxia of the hindquarters with lateral side-to-side swaying and culminated in total paralysis. Two animals were sent for necropsy in 2009. The main histopathological findings were severe myelitis in the lumbar spinal cord with perivascular cuffing and white matter necrosis, severe nonsuppurative meningitis with thrombi in subarachnoid blood vessels, and intravascular presence of multiple adult parasites. From the morphological characteristics of the parasites and location in the leptomeninges, the parasite was identified as the nematode Gurltia paralysans.São relatados dois surtos de paralisia progressiva dos membros posteriores em gatos (Felis catus, causada por meningomielite parasitária no Uruguai. Os estudos de casos ocorreram entre os anos 2008 e 2009, respectivamente, nas zonas rurais de Fray Bentos (33º 07' 40,39" S e foram caracterizados por paralisia dos membros posteriores. Esta paralisia era progressiva e tinha evolução crônica de aproximadamente 12 meses, até que os animais vinham a óbito ou eram eutanasiados. Os sintomas clínicos começaram com ataxia dos membros posteriores, com movimentos laterais, terminado em paralisia total. Em 2009, dois animais foram encaminhados para necropsia. Os achados histopatológicos foram caracterizados por severa mielite na medula espinhal lombar com manguitos perivasculares linfocitarios e necrose da substância branca, severa meningite não supurativa com trombos nos vasos sanguíneos subaracnóideos, e presença intravascular de m

  9. Prophylactic treatment of flea-infested cats with an imidacloprid/flumethrin collar to forestall infection with Dipylidium caninum

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    Fourie Josephus J

    2012-07-01

    Full Text Available Abstract Background The objective of the study was to determine the sustained effectiveness of 10% imidacloprid (w/w and 4.5% flumethrin (w/w incorporated in a slow-release matrix collar in preventing Dipylidium caninum infection in cats following repeated laboratory-infestations with fleas infected with metacestodes. Methods Efficacy against infection with D. caninum was evaluated by infesting 16 cats with the flea Ctenocephalides felis felis infected with metacestodes of the tapeworm. Medicated collars were fitted to 8 of the cats and infestation of each cat with 200 fleas from a suitably infected batch commenced 7 days later and continued at weekly intervals until Day 28. Efficacy against fleas was evaluated 24 h after each infestation. Infection of the cats with D. caninum was verified by daily examination of the cats’ faeces and immediate surroundings for proglottids from Day 21 to Day 60. Calculation of the prophylactic effectiveness of the collars in preventing infection of the cats with D. caninum was based on the difference in the geometric mean number of scoleces recovered from the gastrointestinal tracts of collared compared to untreated cats at necropsy on Day 61. Results Efficacy of the collars against infestation of the cats with fleas was 99.9% on Day 7 and 100% at each subsequent weekly assessment. Infection of the fleas with metacestodes was ≥40% in 7 to 13 day old fleas, but progressively decreased thereafter. At necropsy all the control cats were infected with D. caninum and harboured between 19 and 346 scoleces with a geometric mean of 58.3. A single treated cat was infected and harboured 2 scoleces. Effective prevention of infection with D. caninum, based on a comparison of the geometric mean numbers of scoleces recovered from control and treated cats, was 99.7%. Conclusion The insecticidal components of the medicated collars are capable of rapidly eliminating newly-acquired infestations of fleas that are infected

  10. Efficacy of an imidacloprid/flumethrin collar against fleas, ticks, mites and lice on dogs

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    Stanneck Dorothee

    2012-05-01

    Full Text Available Abstract Background The studies reported here were conducted to ascertain the efficacy of imidacloprid/flumethrin incorporated in a slow-release matrix collar, against infestations of dogs by fleas, ticks, mites and lice. Efficacy was evaluated against the flea Ctenocephalides felis felis, the ticks Rhipicephalus sanguineus, Ixodes ricinus, Ixodes scapularis, Dermacentor reticulatus and Dermacentor variabilis, the mite Sarcoptes scabiei and the biting louse Trichodectes canis. Methods Groups of collar-treated dogs (n = 7–10 were infested with fleas and/or ticks at monthly intervals at least, over a period of up to 8 months. Efficacy against fleas was evaluated 24 to 48 h after treatment and 24 h after each re-infestation. Efficacy against ticks was evaluated at 48 h (acaricidal, 6 h (repellent and 48 h (sustained after infestation. The effect of regular shampooing or immersion in water on the efficacy of the collars was also tested. Efficacy against flea larvae was assessed by incubating blanket samples after dog contact with viable flea eggs. Effectiveness against lice and mites was evaluated after treatment of naturally infested animals. With the exception of the mites, efficacy was calculated by comparison with untreated negative control groups. Results Efficacy against fleas (24 h generally exceeded 95%, and against flea larvae it exceeded 99% for 8 months. Sustained acaricidal (48 h efficacy, covering a period of 8 months was 100% against I. ricinus, starting 2 days after treatment (in vivo, and 100% against I. scapularis (in vitro, above 97% against R. sanguineus, generally above 97% against D. reticulatus and above 90% for D. variabilis. Repellent (6 h efficacy 2 days after treatment and continuing for 8 months was consistently 100% against I. ricinus, and above 90% against R. sanguineus. Regular shampooing affected efficacy against fleas and ticks to a lesser extent than regular immersion in water. The

  11. Predation on dormice in Italy

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    Dino Scaravelli

    1995-05-01

    Full Text Available Abstract The authors analyse available data on the impact of predators on Dormouse populations in Italy. Dormice are found in the diet of 2 snakes (Vipera berus and V. aspis, 2 diurnal birds of prey (Buteo buteo and Aquila chrysaetos, 6 owls (Tyto alba, Strix aluco, Asio otus, Athene noctua, Bubo bubo and Glaucidium passerinum and 9 mammals (Rattus rattus, Ursus arctos, Canis lupus, Vulpes vulpes, Martes martes, M. foina, Meles meles, Felis silvestris and Sus scrofa in a variable percentage of the prey taken. Only Dryomys nitedula was never encountered as a prey item. The most common prey is Muscardinus avellanarius. There are significative regional differences in predation between bioclimatic areas of the Italian peninsula. The contribution of studies on predation to knowledge of Myoxid distribution is discussed. Riassunto Predazione di Mioxidi in Italia - Sono analizzati i dati pubblicati sull'impatto dei predatori sulle popolazioni di Myoxidae in Italia. Myoxidae sono stati riscontrati nelle diete di 2 serpenti (Vipera berus e V. aspis, 2 rapaci diurni (Buteo buteo e Aquila chrysaetos, 6 notturni (Tyto alba, Strix aluco, Asio otus, Athene noctua, Bubo bubo e Glaucidium passerinum e 9 mammiferi (Rattus rattus, Ursus arctos, Canis lupus, Vulpes vulpes, Martes martes, M foina, Meles meles, Felis silvestris e Sus scrofa in percentuale variabile nella comunità di prede. Solo Dryomys nitedula non è mai stato incontrato come preda. La specie piu comunemente predata risulta Muscardinus avellanarius. Sono discusse le

  12. Parasitos de aves e mamíferos silvestres em cativeiro no estado de Pernambuco

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    Pauline Marie de Souza Santos

    2015-09-01

    Full Text Available Resumo: Os animais silvestres são hospedeiros de uma grande variedade de parasitos que podem interferir em sua conservação ex situ. O objetivo deste estudo foi identificar os parasitos gastrointestinais (PGI e ectoparasitos dos animais do Centro de Triagem de Animais Silvestres (CETAS do Instituto Brasileiro de Meio Ambiente e Recursos Naturais Renováveis (IBAMA de Recife, Pernambuco, além de determinar os aspectos do manejo em cativeiro que possam estar relacionados com os parasitos identificados. Foram coletados ectoparasitos e amostras fecais de 223 aves e mamíferos, as quais foram processadas pelos métodos: microscopia direta, flutuação e sedimentação. Helmintos e/ou protozoários foram detectados em 91 (40,8% amostras fecais, sendo 64 (70,3% de aves e 27 (29,7% de mamíferos. Ovos de Capillaria sp., Ascaridida, Spirurida e oocistos de Eimeria sp. foram detectados nas amostras fecais das aves, enquanto ovos de Trichuris trichiura, Strongyloides sp., Toxocara canis, Ancylostoma sp., Strongylida e oocistos de Coccídios foram encontrados nas amostras fecais de mamíferos. Os ectoparasitos identificados em aves foram Colpocephalum turbinatum, Kurodaia (Kurodaia fulvofasciata, Halipeurus sp., Naubates sp., Saemundssonia sp., Austromenopon sp., Paragoniocotes sp., Brueelia sp., Myrsidea sp. and Pseudolynchia sp., enquanto em mamíferos os ectoparasitos identificados foram Rhipicephalus sanguineus, Amblyomma varium, A. calcaratum, A. nodosum, Ornithodoros talaje e Ctenocephalides felis felis. A. calcaratum e O. talaje são registrados pela primeira vez em Pernambuco e T. tetradactyla é apresentado como novo hospedeiro de O. talaje. Nenhum dos animais estudados apresentou sinais clínicos em decorrência da infecção/infestação parasitária. Parasitos com potencial zoonótico como T. trichiura, Strongyloides sp., T. canis e Ancylostoma sp. foram identificados em primatas não humanos e carnívoros. Precárias condições estruturais

  13. Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis.

    Science.gov (United States)

    Knapp, Jenny; Umhang, Gérald; Poulle, Marie-Lazarine; Millon, Laurence

    2016-05-15

    Studying the environmental occurrence of parasites of concern for humans and animals based on coprosamples is an expanding field of work in epidemiology and the ecology of health. Detecting and quantifying Toxocara spp. and Echinococcus multilocularis, two predominant zoonotic helminths circulating in European carnivores, in feces may help to better target measures for prevention. A rapid, sensitive, and one-step quantitative PCR (qPCR) allowing detection of E. multilocularis and Toxocara spp. was developed in the present study, combined with a host fecal test based on the identification of three carnivores (red fox, dog, and cat) involved in the life cycles of these parasites. A total of 68 coprosamples were collected from identified specimens from Vulpes vulpes, Canis lupus familiaris, Canis lupus, Felis silvestris catus, Meles meles, Martes foina, and Martes martes With DNA coprosamples, real-time PCR was performed in duplex with a qPCR inhibitor control specifically designed for this study. All the coprosample host identifications were confirmed by qPCR combined with sequencing, and parasites were detected and confirmed (E. multilocularis in red foxes and Toxocara cati in cats; 16% of samples presented inhibition). By combining parasite detection and quantification, the host fecal test, and a new qPCR inhibitor control, we created a technique with a high sensitivity that may considerably improve environmental studies of pathogens. PMID:26969697

  14. Wild Rodents as Experimental Intermediate Hosts of Lagochilascaris minor Leiper, 1909

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    Julieta Machado Paçô

    1999-07-01

    Full Text Available A total of 25 specimens of Cavia porcellus (guinea pig, 5 Dasyprocta agouti (agouti, and 22 Calomys callosus (vesper mice were inoculated with infective eggs of Lagochilascaris minor. The inoculum was prepared with embryonated eggs and orally administered to each individual animal through an esophagus probe. In parallel, 100 specimens of Felis catus domesticus were individually fed with 55-70 nodules containing 3rd-stage larvae encysted in tissues of infected rodents. Animals were examined and necropsied at different time intervals. The migration and encystment of L3 larva was observed in viscera, skeletal muscle, adipose and subcutaneous tissues from all rodents. Adult worms localized at abscesses in the cervical region, rhino, and oropharynx were recovered from domestic cats inoculated with infected rodent tissues. Through this study we can conclude that: (1 wild rodents act as intermediate hosts, characterizing this ascarid heteroxenic cycle; (2 in natural conditions rodents could possibly act as either intermediate hosts or paratenic hosts of Lagochilascaris minor; (3 despite the occurrence of an auto-infecting cycle, in prime-infection of felines (definite hosts the cycle is only completed when intermediate hosts are provided; and (4 in the wild, rodents could serve as a source of infection for humans as they are frequently used as food in regions with the highest incidence of human lagochilascariasis.

  15. Adaptive Surface Reconstruction Based on Tensor Product Algebraic Splines

    Institute of Scientific and Technical Information of China (English)

    Xinghua Song; Falai Chen

    2009-01-01

    Surface reconstruction from unorganized data points is a challenging problem in Computer Aided Design and Geometric Modeling. In this paper, we extend the mathematical model proposed by Juttler and Felis (Adv. Comput. Math., 17 (2002), pp. 135-152) based on tensor product algebraic spline surfaces from fixed meshes to adaptive meshes. We start with a tensor product algebraic B-spline surface defined on an initial mesh to fit the given data based on an optimization approach. By measuring the fitting errors over each cell of the mesh, we recursively insert new knots in cells over which the errors are larger than some given threshold, and construct a new algebraic spline surface to better fit the given data locally. The algorithm terminates when the error over each cell is less than the threshold. We provide some examples to demonstrate our algorithm and compare it with Jiittler's method. Examples suggest that our method is effective and is able to produce reconstruction surfaces of high quality.AMS subject classifications: 65D17

  16. Cats of the Pharaohs: Genetic Comparison of Egyptian Cat Mummies to their Feline Contemporaries.

    Science.gov (United States)

    Kurushima, Jennifer D; Ikram, Salima; Knudsen, Joan; Bleiberg, Edward; Grahn, Robert A; Lyons, Leslie A

    2012-10-01

    The ancient Egyptians mummified an abundance of cats during the Late Period (664 - 332 BC). The overlapping morphology and sizes of developing wildcats and domestic cats confounds the identity of mummified cat species. Genetic analyses should support mummy identification and was conducted on two long bones and a mandible of three cats that were mummified by the ancient Egyptians. The mummy DNA was extracted in a dedicated ancient DNA laboratory at the University of California - Davis, then directly sequencing between 246 and 402 bp of the mtDNA control region from each bone. When compared to a dataset of wildcats (Felis silvestris silvestris, F. s. tristrami, and F. chaus) as well as a previously published worldwide dataset of modern domestic cat samples, including Egypt, the DNA evidence suggests the three mummies represent common contemporary domestic cat mitotypes prevalent in modern Egypt and the Middle East. Divergence estimates date the origin of the mummies' mitotypes to between two and 7.5 thousand years prior to their mummification, likely prior to or during Egyptian Predyanstic and Early Dynastic Periods. These data are the first genetic evidence supporting that the ancient Egyptians used domesticated cats, F. s. catus, for votive mummies, and likely implies cats were domesticated prior to extensive mummification of cats. PMID:22923880

  17. Emendation of the family Chlamydiaceae: proposal of a single genus, Chlamydia, to include all currently recognized species.

    Science.gov (United States)

    Sachse, Konrad; Bavoil, Patrik M; Kaltenboeck, Bernhard; Stephens, Richard S; Kuo, Cho-Chou; Rosselló-Móra, Ramon; Horn, Matthias

    2015-03-01

    The family Chlamydiaceae (order Chlamydiales, phylum Chlamydiae) comprises important, obligate intracellular bacterial pathogens of humans and animals. Subdivision of the family into the two genera Chlamydia and Chlamydophila has been discussed controversially during the past decade. Here, we have revisited the current classification in the light of recent genomic data and in the context of the unique biological properties of these microorganisms. We conclude that neither generally used 16S rRNA sequence identity cut-off values nor parameters based on genomic similarity consistently separate the two genera. Notably, no easily recognizable phenotype such as host preference or tissue tropism is available that would support a subdivision. In addition, the genus Chlamydophila is currently not well accepted and not used by a majority of research groups in the field. Therefore, we propose the classification of all 11 currently recognized Chlamydiaceae species in a single genus, the genus Chlamydia. Finally, we provide emended descriptions of the family Chlamydiaceae, the genus Chlamydia, as well as the species Chlamydia abortus, Chlamydia caviae and Chlamydia felis.

  18. Evolutionary dynamics of endogenous feline leukemia virus proliferation among species of the domestic cat lineage

    International Nuclear Information System (INIS)

    Endogenous feline leukemia viruses (enFeLVs) occur in the germ lines of the domestic cat and related wild species (genus Felis). We sequenced the long terminal repeats and part of the env region of enFeLVs in domestic cats and five wild species. A total of 305 enFeLV sequences were generated across 17 individuals, demonstrating considerable diversity within two major clades. Distinct proliferations of enFeLVs occurred before and after the black-footed cat diverged from the other species. Diversity of enFeLVs was limited for the sand cat and jungle cat suggesting that proliferation of enFeLVs occurred within these species after they diverged. Relationships among enFeLVs were congruent with host species relationships except for the jungle cat, which carried only enFeLVs from a lineage that recently invaded the germline (enFeLV-AGTT). Comparison of wildcat and domestic cat enFeLVs indicated that a distinctive germ line invasion of enFeLVs has not occurred since the cat was domesticated.

  19. Repetitive sequences in Eurasian lynx (Lynx lynx L.) mitochondrial DNA control region.

    Science.gov (United States)

    Sindičić, Magda; Gomerčić, Tomislav; Galov, Ana; Polanc, Primož; Huber, Duro; Slavica, Alen

    2012-06-01

    Mitochondrial DNA (mtDNA) control region (CR) of numerous species is known to include up to five different repetitive sequences (RS1-RS5) that are found at various locations, involving motifs of different length and extensive length heteroplasmy. Two repetitive sequences (RS2 and RS3) on opposite sides of mtDNA central conserved region have been described in domestic cat (Felis catus) and some other felid species. However, the presence of repetitive sequence RS3 has not been detected in Eurasian lynx (Lynx lynx) yet. We analyzed mtDNA CR of 35 Eurasian lynx (L. lynx L.) samples to characterize repetitive sequences and to compare them with those found in other felid species. We confirmed the presence of 80 base pairs (bp) repetitive sequence (RS2) at the 5' end of the Eurasian lynx mtDNA CR L strand and for the first time we described RS3 repetitive sequence at its 3' end, consisting of an array of tandem repeats five to ten bp long. We found that felid species share similar RS3 repetitive pattern and fundamental repeat motif TACAC.

  20. Reconciling actual and perceived rates of predation by domestic cats

    Science.gov (United States)

    McDonald, Jennifer L; Maclean, Mairead; Evans, Matthew R; Hodgson, Dave J

    2015-01-01

    The predation of wildlife by domestic cats (Felis catus) is a complex problem: Cats are popular companion animals in modern society but are also acknowledged predators of birds, herpetofauna, invertebrates, and small mammals. A comprehensive understanding of this conservation issue demands an understanding of both the ecological consequence of owning a domestic cat and the attitudes of cat owners. Here, we determine whether cat owners are aware of the predatory behavior of their cats, using data collected from 86 cats in two UK villages. We examine whether the amount of prey their cat returns influences the attitudes of 45 cat owners toward the broader issue of domestic cat predation. We also contribute to the wider understanding of physiological, spatial, and behavioral drivers of prey returns among cats. We find an association between actual prey returns and owner predictions at the coarse scale of predatory/nonpredatory behavior, but no correlation between the observed and predicted prey-return rates among predatory cats. Cat owners generally disagreed with the statement that cats are harmful to wildlife, and disfavored all mitigation options apart from neutering. These attitudes were uncorrelated with the predatory behavior of their cats. Cat owners failed to perceive the magnitude of their cats’ impacts on wildlife and were not influenced by ecological information. Management options for the mitigation of cat predation appear unlikely to work if they focus on “predation awareness” campaigns or restrictions of cat freedom. PMID:26306163

  1. First findings and prevalence of adult heartworms (Dirofilaria immitis) in wild carnivores from Serbia.

    Science.gov (United States)

    Penezić, Aleksandra; Selaković, Sanja; Pavlović, Ivan; Ćirović, Duško

    2014-09-01

    Heartworm (Dirofilaria immitis) is a parasitic roundworm that causes a zoonotic disease known as dirofilariosis. Little is known about the role of wild carnivores serving as reservoirs in nature. Therefore, we examined 738 hearts and lungs of free ranging wild carnivores from Serbia to determine the presence of adult heartworms. During the period 2009-2013, the prevalence in golden jackals (Canis aureus) was 7.32%, in red foxes (Vulpes vulpes) 1.55%, in wolves (Canis lupus) 1.43%, and in wild cats (Felis silvestris) 7.69%. No adult heartworm specimens were found in beech martens (Martes foina), stone martens (Martes martes), European polecats (Mustela putorius), badgers (Meles meles) or otter (Lutra lutra). The highest recorded prevalence was in 2013 (7.30%) and the lowest in 2012 (1.6%). In jackals, the prevalence was higher in males (10%) than in females (4.06%), while in foxes the prevalence was 1.75% in males and 1.26% in females. The most infected host was a wolf in which 37 adult specimens were found. Because of the potentially significant role in the life cycle of D. immitis, populations of wild carnivores in Europe should be further examined and tested for heartworm infections. PMID:24951168

  2. Small carnivores of Biligiri Rangaswamy Temple Tiger Reserve, Karnataka, India

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    Honnavalli N. Kumara

    2014-11-01

    Full Text Available During the present study in Biligiri Rangaswamy Temple Tiger Reserve (BRT, nine species of small carnivores viz., Jungle Cat Felis chaus, Rusty-spotted Cat Prionalilurus rubiginosus, Leopard Cat Prionailurus bengalensis, Small Indian Civet Viverricula indica, Asian Palm Civet Paradoxurus hermaphroditus, Striped-necked Mongoose Herpestes vitticollis, Ruddy Mongoose Herpestes smithii, Common Mongoose Herpestes edwardsii and Smooth-coated Otter Lutrogale perspicillata, were recorded using camera-trapping technique, transect walks, and night surveys. Vegetation type strongly influences the presence and abundance of each species. The most sightings of small carnivores occurred in dry deciduous forests. Among all the species, the Asian Palm Civet was the most abundant and was followed by the small Indian Civet. Compared to many other forests or regions in India, the sight records of the Rusty-spotted Cat were relatively higher in BRT. Although we were unable to use statistical methods to search for higher levels of interdependencies between forest types and small carnivore abundance, our study sheds light on patterns of small carnivore distribution in this unique habitat which bridges the Western Ghats and the Eastern Ghats.

  3. Environmental Aspects of Domestic Cat Care and Management: Implications for Cat Welfare

    Science.gov (United States)

    Stella, Judith L.

    2016-01-01

    Domestic cats (Felis silvestris catus) are the most commonly kept companion animals in the US with large populations of owned (86 million), free-roaming (70 million), research (13,000), and shelter (2-3 million) cats. Vast numbers of cats are maintained in homes and other facilities each year and are reliant on humans for all of their care. Understanding cat behavior and providing the highest quality environments possible, including positive human-cat interactions, based on research could help improve the outcomes of biomedical research, shelter adoptions, and veterinary care, as well as overall cat welfare. Often, however, cats' needs are inadequately met in homes and some aspects may also not be well met in research colonies and shelters, despite the fact that similar problems are likely to be encountered in all of these environments. This paper provides a brief overview of common welfare challenges associated with indoor housing of domestic cats. Essential considerations for cage confinement are reviewed, along with implications of poor cat coping, such as weakening of the human-animal bond and relinquishment to shelters. The important role that environmental management plays in cat behavior and welfare outcomes is explored along with the need for additional research in key areas.

  4. Prevalence and Risk Factors of Intestinal Parasites in Cats from China

    Science.gov (United States)

    2015-01-01

    The prevalence of intestinal parasites in cats from China was largely unknown prior to this study. The aim of the present study was to investigate the presence of intestinal parasites in cats from central China and also identify risk factors for parasitism. Fecal samples from 360 cats were examined using sugar flotation procedure and fecal smear test by microscope. Cats had mixed two or three kinds of parasites infections. Of the 360 cats feces, intestinal parasites positive feces were 149 (41.39%). 64 (17.78%) were infected with Toxocara cati, 61 (16.94%) with Isospora felis, 41 (11.39%) with Isospora rivolta, 33 (9.17%) with Paragonimus, 23 (6.39%) with hookworms, 11 (3.06%) with Toxoplasma-like oocysts, 10 (2.78%) with Trichuris, 4 (1.11%) with lungworm, 2 (0.56%) with Sarcocystis, and 1 (0.28%) with Trematode. The cats' living outdoor was identified as risk factor by statistical analysis. These results provide relevant basic data for assessing the infection of intestinal parasites in cats from central region of China. In conclusion, there was high prevalence of intestinal parasites in cats from China. PMID:26078975

  5. Prevalence and Risk Factors of Intestinal Parasites in Cats from China

    Directory of Open Access Journals (Sweden)

    Yurong Yang

    2015-01-01

    Full Text Available The prevalence of intestinal parasites in cats from China was largely unknown prior to this study. The aim of the present study was to investigate the presence of intestinal parasites in cats from central China and also identify risk factors for parasitism. Fecal samples from 360 cats were examined using sugar flotation procedure and fecal smear test by microscope. Cats had mixed two or three kinds of parasites infections. Of the 360 cats feces, intestinal parasites positive feces were 149 (41.39%. 64 (17.78% were infected with Toxocara cati, 61 (16.94% with Isospora felis, 41 (11.39% with Isospora rivolta, 33 (9.17% with Paragonimus, 23 (6.39% with hookworms, 11 (3.06% with Toxoplasma-like oocysts, 10 (2.78% with Trichuris, 4 (1.11% with lungworm, 2 (0.56% with Sarcocystis, and 1 (0.28% with Trematode. The cats’ living outdoor was identified as risk factor by statistical analysis. These results provide relevant basic data for assessing the infection of intestinal parasites in cats from central region of China. In conclusion, there was high prevalence of intestinal parasites in cats from China.

  6. Variability in assays used for detection of lentiviral infection in bobcats (Lynx rufus), pumas (Puma concolor), and ocelots (Leopardus pardalis)

    Science.gov (United States)

    Franklin, S.P.; Troyer, J.L.; TerWee, J.A.; Lyren, L.M.; Kays, R.W.; Riley, S.P.D.; Boyce, W.M.; Crooks, K.R.; VandeWoude, S.

    2007-01-01

    Although lentiviruses similar to feline immunodeficiency virus (FIV) are known to infect numerous felid species, the relative utility of assays used for detecting lentiviral infection has not been compared for many of these hosts. We tested bobcats (Lynx rufus), pumas (Felis concolor), and ocelots (Leopardus pardalis) for exposure to lentivirus using five different assays: puma lentivirus (PLV), African lion lentivirus (LLV), and domestic cat FIV-based immunoblots, a commercially available enzyme-linked immunosorbent assay (ELISA) kit, and nested polymerase chain reaction (PCR). Puma lentivirus immunoblots identified more seropositive individuals than the other antibody-detection assays. The commercial ELISA provided a fair ability to recognize seropositive samples when compared with PLV immunoblot for screening bobcats and ocelots, but not pumas. Polymerase chain reaction identified fewer positive samples than PLV immunoblot for all three species. Immunoblot results were equivalent whether the sample tested was serum, plasma, or whole blood. The results from this study and previous investigations suggest that the PLV immunoblot has the greatest ability to detect reactive samples when screening wild felids of North America and is unlikely to produce false positive results. However, the commercial ELISA kit may provide ap adequate alternative for screening of some species and is more easily adapted to field conditions. ?? Wildlife Disease Association 2007.

  7. Estimation of Correlation between the Number of Individuals and Biogenic Capacity of the Hunting Terrain 54 Padureni, Forest District Lunca Timişului, for 13 Hunting Species in 2007-2011 Period

    Directory of Open Access Journals (Sweden)

    Dorel Dronca

    2012-05-01

    Full Text Available Integration of the Romania in the EU is imposing a special attention to hunting animals populations. The aim of thepresent paper was to study the quantitative evolution of the hunting population for the 13th species, in the period2007-2011, in the hunting terrain 54 Padureni, from Forest Domain – Lunca Timişului, with a total surface of11,963ha. The paper shows that form the 13th species studied 5 were not identified on this hunting terrain Red Deer(Cervus elaphus L., Wildcat (Felis silvestres L, European Pine Marten (Martes martes L., Least Weasel (Mustelanivolis L. Muskrat (Ondatra zibethica L.. For the other species identified the individuals number evolutiondemonstrated that there is a god correlation between the number of individuals and it-s biogenic capacity. Also thepaper recommends a special attention to natural selection and the efficient use of artificial selection, especially forthe following 3 species: Roe Deer (Capreolus Capreolus L., Wild hog (Sus scrofa L. and Common Pheasant(Phasianus colchicus L..

  8. I. Sexual, individual, and geographical variation in leucosticte tephrocotis, II.Geographical variation among North American mammals, especially in respect to size

    Science.gov (United States)

    Allen, J.A.

    1876-01-01

    Having recently had an opportunity (through the kindness of Professor Baird) of studying with some care the magnificent series of skulls of the North American Mammalia belonging to the National Museum (amounting often to eighty or a hundred specimens of a single species), I have been strongly impressed with the different degrees of variability exhibited by the representatives of the species and genera of even the same family. The variation in size, for instance, with latitude, in the Wolves and Foxes is surprisingly great, amounting in some species (as will be shown later) to 25 per cent. of the average size of the species, while in other species of the Ferae it is almost nil. Contrary to the general supposition, the variation in size among representatives of the same species is not always a decrease with the decrease of the latitude of the locality, but is in some cases exactly the reverse, in some species there being a very considerable and indisputable increase southward. This, for instance, is very markedly true of some species of Felis and in Procyon lotor. Consequently, the very generally-received impression that in North America the species of Mammalia diminish in size southward, or with the decrease in the latitude (and altitude) of the locality, requires modification. While such is generally the case, the reverse of this too often occurs, with occasional instances also of a total absence of variation in size with locality, to be considered as forming "the exceptions" necessary to "prove the rule".

  9. Synergistic antiviral effect of Galanthus nivalis agglutinin and nelfinavir against feline coronavirus.

    Science.gov (United States)

    Hsieh, Li-En; Lin, Chao-Nan; Su, Bi-Ling; Jan, Tong-Rong; Chen, Chi-Min; Wang, Ching-Ho; Lin, Dah-Sheng; Lin, Chung-Tien; Chueh, Ling-Ling

    2010-10-01

    Feline infectious peritonitis (FIP) is a fatal disease in domestic and nondomestic felids caused by feline coronavirus (FCoV). Currently, no effective vaccine is available for the prevention of this disease. In searching for agents that may prove clinically effective against FCoV infection, 16 compounds were screened for their antiviral activity against a local FCoV strain in Felis catus whole fetus-4 cells. The results showed that Galanthus nivalis agglutinin (GNA) and nelfinavir effectively inhibited FCoV replication. When the amount of virus preinoculated into the test cells was increased to mimic the high viral load present in the target cells of FIP cats, GNA and nelfinavir by themselves lost their inhibitory effect. However, when the two agents were added together to FCoV-infected cells, a synergistic antiviral effect defined by complete blockage of viral replication was observed. These results suggest that the combined use of GNA and nelfinavir has therapeutic potential in the prophylaxis and treatment of cats with early-diagnosed FIP.

  10. Feline Immunodeficiency Virus in South America

    Directory of Open Access Journals (Sweden)

    Bruno M. Teixeira

    2012-03-01

    Full Text Available The rapid emergence of AIDS in humans during the period between 1980 and 2000 has led to extensive efforts to understand more fully similar etiologic agents of chronic and progressive acquired immunodeficiency disease in several mammalian species. Lentiviruses that have gene sequence homology with human immunodeficiency virus (HIV have been found in different species (including sheep, goats, horses, cattle, cats, and several Old World monkey species. Lentiviruses, comprising a genus of the Retroviridae family, cause persistent infection that can lead to varying degrees of morbidity and mortality depending on the virus and the host species involved. Feline immunodeficiency virus (FIV causes an immune system disease in domestic cats (Felis catus involving depletion of the CD4+ population of T lymphocytes, increased susceptibility to opportunistic infections, and sometimes death. Viruses related to domestic cat FIV occur also in a variety of nondomestic felids. This is a brief overview of the current state of knowledge of this large and ancient group of viruses (FIVs in South America.

  11. Use of polymerase chain reaction and enzymatic cleavage in the identification of Helicobacter spp. in gastric mucosa of human beings from North Paraná, Brazil

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    Camargo PL

    2003-01-01

    Full Text Available Helicobacter pylori is the most common gastric bacteria of human beings. Animal-borne helicobacter have been associated with gastritis, ulceration, and gastric mucosa-associated lymphoid-tissue lymphoma in people. We attempted to identify the species of Helicobacter spp. that infect human beings in north Paraná, Brazil. Samples of gastric mucosa from 38 dyspeptic patients were analyzed by optic microscopy on silver stained slides, polimerase chain reaction (PCR, and enzymatic cleavage. Genus and species-specific primers to H. pylori, H. heilmannii, H. felis, and consensual primers to H. bizzozeronii or H. salomonis were used. The PCR products were submitted to enzymatic cleavage by VspI (Helicobacter spp. product and HinfI (species products enzymes. Thirty-two out of 38 patients evaluated had 3.2 to 5 µm long bacteria that resembled H. pylori in Warthin-Starry stained slides and were positive to the genus Helicobacter by PCR. In 30 of these patients the bacteria were identified as H. pylori. Two samples positive by silver stain were negative to all species tested by PCR. None of the 38 samples was positive to animal-origin helicobacter species. These results show that PCR and enzymatic restriction are practical methods to identify the species of helicobacters present in gastric mucosa of human beings. People in north Paraná appear to be infected mostly with H. pylori.

  12. Hellsgate Winter Range Mitigation Project; Long-term Management Plan, Project Report 1993, Final Draft.

    Energy Technology Data Exchange (ETDEWEB)

    Berger, Matthew T.

    1994-01-01

    A study was conducted on the Hellsgate Winter Range Mitigation Project area, a 4,943 acre ranch purchased for mitigating some habitat losses associated with the original construction of Grand Coulee Dam and innundation of habitat by Lake Roosevelt. A Habitat Evaluation Procedure (HEP) study was used to determine habitat quality and quantity baseline data and future projections. Target species used in the study were sharp-tailed grouse (Tympanuchus phasianellus), mule deer (Odocoileus hemoinus), mink (Mustela vison), spotted sandpiper (Actiius colchicus), bobcat (Felis reufs), blue grouse (Dendragapus obscurus), and mourning dove (Zenaida macroura). From field data collected, limiting life values or HSI's (Habitat Suitability Index's) for each indicator species was determined for existing habitats on project lands. From this data a long term management plan was developed. This report is designed to provide guidance for the management of project lands in relation to the habitat cover types discussed and the indicator species used to evaluate these cover types. In addition, the plan discusses management actions, habitat enhancements, and tools that will be used to enhance, protect and restore habitats to desired conditions. Through planned management actions biodiversity and vegetative structure can be optimized over time to reduce or eliminate, limiting HSI values for selected wildlife on project lands.

  13. The Hybrid Nature of 0846+51W1: a BL Lac Object with a Narrow Line Seyfert 1 Nucleus

    Institute of Scientific and Technical Information of China (English)

    Hong-Yan Zhou; Ting-Gui Wang; Xiao-Bo Dong; Cheng Li; Xue-Guang Zhang

    2005-01-01

    We found a NLS1 nucleus in the extensively studied eruptive BL Lac object, 0846+51W1, out of a large sample of NLS1s compiled from the spectroscopic dataset of SDSS DR1. Its optical spectrum can be well decomposed into three components: a power law component from the relativistic jet, a stellar component from the host galaxy, and a component from a typical NLS1 nucleus. The emission line properties of 0846+51Wl, FWHM (Hβ)∽- 1710 km s-1 and [OⅢ]λ5007 /Hβ∽-0.32from its SDSS spectrum observed when it was in the faint state, fulfil the conventional definition of NLS1. Strong FelI emission is detected in the SDSS spectrum,which is also typical of NLS1s. We tried to estimate its central black hole mass using various techniques and found that 0846+51W1 is very likely emitting at a few × 10% of the Eddington luminosity. We speculate that Seyfert-like nuclei, including NLS1s, might be concealed in a significant fraction of BL Lacs but have remained largely unobserved because, often, their optical-UV continuum is overwhelmed by the synchrotron emission.

  14. Electrochemical reactions and cathode properties of Fe-doped Li2O for the hermetically sealed lithium peroxide battery

    Science.gov (United States)

    Harada, Kosuke; Hibino, Mitsuhiro; Kobayashi, Hiroaki; Ogasawara, Yoshiyuki; Okuoka, Shin-ichi; Yonehara, Koji; Ono, Hironobu; Sumida, Yasutaka; Yamaguchi, Kazuya; Kudo, Tetsuichi; Mizuno, Noritaka

    2016-08-01

    Fe-doped Li2O (FDL) is synthesized mechanochemically and is demonstrated as a new Co-free cathode material for use in sealed Li2O2 batteries, which have been proposed as high energy density batteries. Fe3+ ions are substitutionally doped into the Li sites in an antifluorite-type Li2O structure to create FDL. The FDL consists of (Li0.82Fe0.06)2O (d-FDL) and high-temperature form of Li5FeO4 (o-FDL), in which Fe3+ ions disorderly and orderly arranged, respectively. According to the Mössbauer spectra and quantitative peroxide species analysis, the FDL cathode operates principally based on the redox reaction between O22- and O2-. X-ray diffraction study reveals that the reversible formation of O22- proceeds mainly in the d-FDL. An irreversible side reaction involving the evolution of oxygen gas occurs when the cathode is charged to more than 250 mAh g-1. The FDL (Fe/(Li + Fe) = 10 at%) cathode exhibits a reversible capacity of 200 mAh g-1 over 200 cycles at a current density of 22.5 mA g-1.

  15. Endogenous versus exogenous lithium clearance for evaluation of dopamine-induced changes in renal tubular function

    DEFF Research Database (Denmark)

    Olsen, Niels Vidiendal; Fogh-Andersen, N; Strandgaard, S;

    1996-01-01

    1. The present randomized, double-blind cross-over study compared endogenous and exogenous lithium clearance (CLi) for estimation of the effect of dopamine on tubular sodium reabsorption. Twelve normal, salt-repleted male subjects were investigated on three different occasions with either placebo...... or 450 mg or 600 mg of lithium given in random order at 22.00 hours. After an overnight fast, renal clearance studies were performed during a 1 h baseline period and subsequently during the second hour of an infusion of 3 micrograms min-1 kg-1 of dopamine. 2. Baseline values of endogenous CLi and...... fractional excretion of lithium (FELi) [27.0 (23.5-30.5) ml/min and 24.2 (20.3-28.2)% (means with 95% confidence interval)] were lower than exogenous values [lithium, 450 mg: 32.7 (29.9-35.4) ml/min (P <0.05) and 27.4 (25.2-29.6)% (P <0.05); lithium, 600 mg: 33.4 (29.2-37.6)ml/min (P <0.05) and 28.6 (26...

  16. Distribución y Hospederos de pulgas (Siphonaptera en la Provincia de Ayabaca, Piura - 1999

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    Edwar J Pozo

    2005-10-01

    Full Text Available Estudio descriptivo transversal realizado en la provincia de Ayabaca (zona endémica de peste bubónica, departamento de Piura, Perú; con el objetivo de ampliar los conocimientos sobre las especies de pulgas presentes. Fueron colectados 10 152 especimenes de pulgas en 46 localidades pertenecientes a seis distritos de la provincia de Ayabaca (Suyo, Sapillica, Montero, Paimas, Lagunas y Ayabaca, entre los meses de enero a julio de 1999. El muestreo se realizó seleccionando para cada vivienda un mínimo de cinco ropas de cama y cinco cuyes (Cavia porcellus además de la totalidad de los roedores capturados en las viviendas y en el área silvestre. Ocho especies de pulgas fueron identificadas (Pulex irritans, Tiamastus cavicola, Polygenis litargus, Xenopsylla cheopis, Ctenocephalides felis, Craneopsylla minerva, Leptopsylla segnis y Cediopsylla spillmanni de éstas, P. irritans se reportó en todos los distritos muestreados, seguida de X. cheopis y P. litargus, encontradas en cinco de los seis distritos.

  17. Molecular Identification and Characterization of Cryptosporidium spp. from Mainland China

    Institute of Scientific and Technical Information of China (English)

    LI Guo-qing; XIANG Fei-yu; LIN Rui-qing; Kanu Saidu; XIAO Shu-min; ZHU Xing-quan; XIE Min-quan

    2005-01-01

    Three isolates of the genus Cryptosporidium, namely, Guangdong isolate, Anhui isolate and Jiangsu isolate from Mainland China, were identified and characterized genetically utilizing nuclear DNA regions of the small subunit of ribosomal RNA (SSU rRNA) and heat shock protein 70 gene (HSP70) as genetic markers. These two regions were amplified by PCR from DNA extracted from oocysts and amplicons of approximately 290 bp and 450 bp were produced, respectively. The amplicons were purified, cloned and sequenced. Sequences of 446 bp and 290-292 bp were obtained for the SSU rRNA and HSP70 regions, respectively. The obtained SSU rRNA and HSP70 sequences representing the three Cryptosporidium isolates were compared with those retrieved from the DNA database. Genetic analyses using either DNA region revealed that members of Cryptosporidium formed two clusters, with C. parvum, C. wariri, C. felis and C. meleagridis clustered together, while C. andersoni, C. muris and C. serpentis belong to the other cluster. Based on SSU rRNA and HSP70 sequences, both Guangdong and Anhui isolates of Cryptosporidium were identified as C. muris of the calf genotype (i.e., C. andersoni), whereas the Jiangsu isolate was identified as C. parvum of the calf genotype. The findings of the present study should have important implications for the diagnosis and control of Cryptosporidium infections in both humans and animals in China.

  18. Detection of flea-borne Rickettsia species in the Western Himalayan region of India

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    R Chahota

    2015-01-01

    Full Text Available Human infections by various rickettsial species are frequently reported globally. We investigated a flea-borne rickettsial outbreak infecting 300 people in Western Himalayan region of India. Arthropod vectors (ticks and fleas and animal and human blood samples from affected households were analysed by gltA and ompB genes based polymerase chain reaction (PCR. Rat flea (Ceratophyllus fasciatus samples were found harbouring a Rickettsia sp. Phylogenetic analysis based on gltA gene using PHYLIP revealed that the detected Rickettsia sp. has 100% identity with SE313 and RF2125 strains of Rickettsia sp. of flea origin from Egypt and Thai-Myanmar border, respectively and cf1 and 5 strains from fleas and lice from the USA. But, the nucleotide sequence of genetically variable gene ompB of R14 strain was found closely related to cf9 strain, reported from Ctenocephalides felis fleas. These results highlight the public health importance of such newly discovered or less recognised Rickettsia species/strains, harboured by arthropod vectors like fleas.

  19. A deletion in FOXN1 is associated with a syndrome characterized by congenital hypotrichosis and short life expectancy in Birman cats.

    Directory of Open Access Journals (Sweden)

    Marie Abitbol

    Full Text Available An autosomal recessive syndrome characterized by congenital hypotrichosis and short life expectancy has been described in the Birman cat breed (Felis silvestris catus. We hypothesized that a FOXN1 (forkhead box N1 loss-of-function allele, associated with the nude phenotype in humans, mice and rats, may account for the syndrome observed in Birman cats. To the best of our knowledge, spontaneous mutations in FOXN1 have never been described in non-human, non-rodent mammalian species. We identified a recessive c.1030_1033delCTGT deletion in FOXN1 in Birman cats. This 4-bp deletion was associated with the syndrome when present in two copies. Percentage of healthy carriers in our French panel of genotyped Birman cats was estimated to be 3.2%. The deletion led to a frameshift and a premature stop codon at position 547 in the protein. In silico, the truncated FOXN1 protein was predicted to lack the activation domain and critical parts of the forkhead DNA binding domain, both involved in the interaction between FOXN1 and its targets, a mandatory step to promote normal hair and thymic epithelial development. Our results enlarge the panel of recessive FOXN1 loss-of-function alleles described in mammals. A DNA test is available; it will help owners avoid matings at risk and should prevent the dissemination of this morbid mutation in domestic felines.

  20. Current Status of Mammals and Reptiles at Hub Dam Area, Sindh / Balochistan, Pakistan

    Directory of Open Access Journals (Sweden)

    Abeda Begum

    2013-12-01

    Full Text Available During the present study in 2012, a total of twenty four mammalian species were recorded belonging to 5 orders and 10 families; out of these, 8 species are less common, 2 species are rare, while 14 species are common in Hub Dam area. Twenty five reptilian species belonging to 3 orders and 12 families were also recorded from the area. Three species of mammalian Urial (Ovis vignei, Chinkara/Indian Gazelle (Gazella bennettii and Jungle Cat (Felis chaus, one reptilian species Common Krait (Bungarus caeruleus were recorded as rare from the study area during 2012. During the present study, nine mammalian species Wild Goat/Sindh Ibex (Capra aegagrus, Urial (Ovis vignei, Chinkara/Indian Gazelle (Gazella bennettii, Indian Hedgehog (Paraechinus micropus, Cape Hare (Lepus capensis, Little Indian Field Mouse (Mus booduga, House Shrew (Sorex thibetanus, Balochistan Gerbil (Gerbillus nanus and Indian Gerbil (Tatera indica and two reptilian Warty Rock Gecko (Cyrtodactylus kachhensis kachhensis and Banded Dwarf Gecko (Tropiocolotes helenae were recorded from the area. As regards threats to mammals and reptiles, these are affected by disturbance. Efforts are being made to conserve the important mammals and reptiles particularly in the protected area especially at Hub Dam.