WorldWideScience

Sample records for bacteriological techniques

  1. Comparison of conventional PCR, quantitative PCR, bacteriological culture and the Warthin Starry technique to detect Leptospira spp. in kidney and liver samples from naturally infected sheep from Brazil.

    Science.gov (United States)

    Fornazari, Felipe; da Silva, Rodrigo Costa; Richini-Pereira, Virginia Bodelão; Beserra, Hugo Enrique Orsini; Luvizotto, Maria Cecília Rui; Langoni, Helio

    2012-09-01

    Leptospirosis is an infectious disease of worldwide importance. The development of diagnostic techniques allows sick animals to be identified, reservoirs to be eliminated and the disease prevented and controlled. The present study aimed to compare different techniques for diagnosing leptospirosis in sheep. Samples of kidney, liver and blood were collected from 465 animals that originated from a slaughterhouse. The sera were analyzed by the Microscopic Agglutination Test (MAT), and kidney and liver samples of seropositive animals were analyzed using four techniques: bacteriological culture, the Warthin Starry (WS) technique, conventional PCR (cPCR), and quantitative PCR (qPCR). With the MAT, 21 animals were positive (4.5%) to serovars Hardjo (n=12), Hebdomadis (n=5), Sentot (n=2), Wolfii (n=1) and Shermani (n=1). Titers were 100 (n=10), 200 (n=2), 400 (n=6) and 1600 (n=3). No animal was positive by bacteriological culture; four animals were positive by the WS technique in kidney samples; six animals were positive by cPCR in kidney samples; and 11 animals were positive by qPCR, eight of which in kidney samples and three in liver. The bacterial quantification revealed a median of 4.3 bacteria/μL in liver samples and 36.6 bacteria/μL in kidney samples. qPCR presented the highest sensitivity among the techniques, followed by cPCR, the WS technique and bacteriological culture. These results indicate that sheep can carry leptospires of the Sejroe serogroup, and demonstrate the efficiency of quantitative PCR to detect Leptospira spp. in tissue samples. Published by Elsevier B.V.

  2. Bacteriological and Physicochemical Qualities of Ebutte River in ...

    African Journals Online (AJOL)

    Michael Horsfall

    ABSTRACT: The bacteriological and physicochemical qualities of Ebutte River in Ebutte Community were carried out to ascertain the variation in the quality of the river between August, 2010 and January, 2011.The bacteriological and physicochemical assessments were studied using the basic microbiological techniques.

  3. Bacteriology of activated sludge

    NARCIS (Netherlands)

    Gils, van H.W.

    1964-01-01

    The bacteriology and biochemistry of activated sludge grown in domestic waste water or fed with synthetic media were studied. The nature of the flocs was investigated by determining morphological and physiological characteristics of many strains isolated.

    Predominant bacteria were

  4. Bacteriologic quality of cellophane - packaged (pure) water ...

    African Journals Online (AJOL)

    The bacteriological (Coliform) quality of six (6) brands of cellophane - packaged drinking water was determined using the Most Probable Number (MPN) technique as described by APHA (1992). Sixty (60) samples were analyzed over a ten week period, with a sample of the six brands analyzed per week. Tap water and Eva ...

  5. Bacteriological studies off Mangalore coast

    Digital Repository Service at National Institute of Oceanography (India)

    Nair, S.; LokaBharathi, P.A.

    Bacteriological studies of sediment and water in the near shore region off Mangalore showed that both limnotolerant and halotolerant bacteria were present. Significant inter-correlation (quantitative and qualitative) could be noted between various...

  6. Assessment of bacteriologic profile of reservoir water in Ekpoma ...

    African Journals Online (AJOL)

    therefore, assesses the bacteriologic profile of water samples in Ekpoma, Edo, Nigeria, considering the vulnerability of the inhabitants to water-borne diseases often associated with unhygienic environment and lack of safe drinking water. The simple random sampling technique was adopted and water samples were ...

  7. Bacteriologically confirmed tuberculosis in children

    International Nuclear Information System (INIS)

    Ozere, I.; Sele, A.; Ozolina, A.

    2005-01-01

    Tuberculosis in children and adults is a growing problem with important public health implications. In Latvia the incidence of tuberculosis (TB) in children up to age 14 has increased from 7,1 per 100000 in 1992 to 28,8 per 100000 in 2003. The diagnosis of TB is confirmed by isolation and identification of M. tuberculosis (MT) from clinical specimen. Confirmation of the disease, however, is difficult in children due to poor bacilli excretion and even under the best circumstances only about 30-40% of pediatric TB cases are proved bacteriologically. Of the 370 pediatric TB cases diagnosed between January 1, 2001 and December 1, 2003 in Latvia, 53 (14,3%) were confirmed bacteriologically. The clinical, radiological, immunological and anamnestic features of confirmed TB can serve as cornerstones for diagnosing of TB, when culture is not available. Objective To evaluate the sensitivity of diagnostic criteria of TB, clinical and radiological manifestation of TB and drug susceptibility of MT isolated also. Methods All consecutive children (53 in total) up to age 14 diagnosed with bacteriologically confirmed TB during 01.01.2001. -01.12.2003. were prospectively evaluated for reasons mentioned above. Results Of the 53 children identified all but one had respiratory tract TB. 17(32,1 %) children were under 4 years of age, 9 (17%) children were 5-9 years old, but 27 (50,9%) patients were 10-14 years old. During evaluation data on TB source case were found in addition in 13 children and total TB contact history was positive in 37 (69,8%) patients. All clinical and radiographical forms of respiratory tract TB were diagnosed. The most common encountered forms were intrathoracic adenopathy in 10 (18,9%) cases and TB pneumonia in 6 (11,3%) cases in children aged 10-14 years. lnthrathoracic adenopathy associated with segmental parenchymal lesion was the most common form in children under 4 years of age -7 (13,2%) cases respectively. Conclusions 1. The clinical and radiological

  8. Bacteriology of diabetic foot lesions

    Directory of Open Access Journals (Sweden)

    Anandi C

    2004-01-01

    Full Text Available Clinical grading and bacteriological study of 107 patients with diabetic foot lesions revealed polymicrobial aetiology in 69 (64.4% and single aetiology in 21 (19.6%. Among 107 patients 62 had ulcer. Of these 31 had mixed aerobes. Twenty six patients with cellulitis and 12 with gangrene had more than 5 types of aerobes and anaerobes such as E.coli, Klebsiella spp., Pseudomonas spp., Proteus spp., Enterobactor spp., Enterococci spp., Clostridium perfringens, Bacteroides spp., Prevotella spp. and Peptostreptococcus spp. It was noted that 50 out of 62 patients with ulcer, and all the patients with cellulitis and gangrene were given surgical management and treated with appropriate antibiotics based on antimicrobial susceptibility testing.

  9. Physicochemical and Bacteriological Characteristics of Rainwater ...

    African Journals Online (AJOL)

    ADOWIE PERE

    This study investigated the physicochemical and bacteriological characteristics of rainwater harvested from three different rooftop sheets in Esan West Local Government Area, Edo State. Parameters investigated include pH, TSS, TDS, Turbidity, Acidity, Ca2+, Fe, Pb, Cr, TBC, TCC and E-coli. The results showed that mean ...

  10. Bacteriological Quality and antimicrobial susceptibility of some ...

    African Journals Online (AJOL)

    In general, the well water samples analyzed in this study were found in unacceptable condition in terms of bacteriological quality. Moreover, the identification of Escherichia coli in some of the wells indicates the safety problem of the water for human consumption. Therefore, it calls for proper disinfection and monitoring of the ...

  11. Water Treatment Technology - Chemistry/Bacteriology.

    Science.gov (United States)

    Ross-Harrington, Melinda; Kincaid, G. David

    One of twelve water treatment technology units, this student manual on chemistry/bacteriology provides instructional materials for twelve competencies. (The twelve units are designed for a continuing education training course for public water supply operators.) The competencies focus on the following areas: waterborne diseases, water sampling…

  12. Bacteriological quality of some pharmaceutical products marketed ...

    African Journals Online (AJOL)

    Bacteriological quality of some pharmaceutical products purchased from open markets, buses and drug stores in Uyo metropolis was studied in order to determine the level of contamination of the drugs. The drug samples examined were Tetracycline capsules, Paracetamol tablets, Ampicillin capsules, Chloroquine tablets, ...

  13. Bacteriological and physicochemical evaluation of syrups produced ...

    African Journals Online (AJOL)

    Bacteriological and physicochemical evaluation of syrups produced from local starch sources. ... The syrups were produced, using acid hydrolysis, from starches obtained from Colocasia esculenta, Zea mays and Manihot palmata. The viable bacterial count gave low counts of Leuconostoc mesenteroides, Bacillus subtills ...

  14. Physicochemical and Bacteriological Properties of Wastewaters ...

    African Journals Online (AJOL)

    The physical, chemical and bacteriological properties of wastewater from four industries located in rural communities of Imo State, Nigeria were determined using standard methods for the examination of water and wastewaters. The results showed that the wastewaters contained high levels of calcium, magnesium and iron.

  15. Physicochemical and Bacteriological Profiles of Borehole Water ...

    African Journals Online (AJOL)

    Similarly, the concentrations of sodium, nitrates, bicarbonates, sulphates and chlorides were within the desirable WHO limits, while those of calcium, magnesium and phosphate are highly against the WHO desirable limit. The bacteriological analyses however indicated the highest bacterial count in Gangire village with ...

  16. Organ bacteriological dynamics in Heterobranchus bidorsalis ...

    African Journals Online (AJOL)

    The effect of dietary intake of Lactobacillus fermentum, Saccharomyces cerevisiae and their combination on selected tissue bacteriology of Heterobranchus bidorsalis juveniles was investigated. Ten experimental diets at 41.0% crude protein were prepared to include Lf1 (basal diet + 10¹cfu/mL Lactobacillus fermentum), Lf2 ...

  17. Bronchiectasis: a bacteriological profile | Bopaka | Pan African ...

    African Journals Online (AJOL)

    The occurrence of bronchiectasis can involve a combination of many environmental factors, including infection. The aim of our work is to determine the bacteriological profile of bronchiectasis. This is a retrospective study of 100 patients hospitalized in between January 2010 and July 2013. The average age was 48 years ...

  18. Bacteriological Evaluation of Kwale General Hospital Environment ...

    African Journals Online (AJOL)

    ... Pharmacy (40.7%) and Theatre (18.5%). This study showed that Kwale General Hospital environment is heavily contaminated and therefore underlies the necessity for regular evaluation of the hospital environment. Keywords: Bacteriological evaluation, hospital, environment. Journal of Medical Laboratory Sciences Vol.

  19. [Dialysate bacteriological quality in a health district].

    Science.gov (United States)

    Lamas, J M; Alonso, M; Sastre, F; Saavedra, J A; García-Trío, G; Palomares, L

    2007-01-01

    There is a serious lack of data in literature on the quality of dialysate used in haemodialysis units throughout Spain and there also exist discrepancies between clinical guides on criteria related to dialysate bacteriological quality. Ascertain bacteriological quality of dialysate used in our area. Descriptive observational studies were carried out monthly and over a period of one year, at two haemodialysis units (unit A: third level public hospital using Monitral-Hospal monitors and unit B: state subsidised non-profit organisation using AK90-Gambro monitors. Tests were performed to determine cultures and endotoxins in water treated with reverse osmosis and in the dialysate. Results are expressed as means (range) and as percentage samples that comply with or deviate from the 2004 recommendations of the Association for Advancement of Medical Instrumentation. Cultures showed 7 (0-53), 100%UE/ml in water treated with reverse osmosis and 725,72 (1,83-2.645), 90%>2 and 16 (0,05-60,87), 70%>2, UE/ml in dialysate from units A and B, respectively. Water treated with reverse osmosis at both units shows good compliance of bacteriological criteria and an acceptable level of endotoxins. The dialysate shows good compliance of bacteriological criteria at unit B and inadequate compliance for unit A. Poor compliance of endotoxins criteria was observed especially in the case of unit A. It would be interesting to have published data on endotoxins levels in dialysate from other dialysis units in Spain, to know if it is possible to achieve the bacteriological quality recommended by the guides using the actual HD monitors without filters for the dialysate and to evaluate from the clinical point of view the utility and efficiency of these filters in conventional HD.

  20. Bacteriological evaluation for one-and two-piece implant design supporting mandibular overdenture.

    Science.gov (United States)

    Abdelwahed, Ahmed; Mahrous, Ahmed I; Abadallah, Mohamed Farouk; Asfour, Hani; Aldawash, Hussien A; Alagha, Ebaa I

    2015-01-01

    This study evaluated and compared the bacteriological effect of two-piece implants and one-piece implants in complete overdenture cases on supporting structures. Ten male completely edentulous patients were selected and randomly divided into two equal groups according to the implant design and surgical technique for this study; Group 1: Patients were rehabilitated with complete mandibular overdenture supported by two-piece implants one on each side of the lower arch following two-stage surgical technique and Group 2: Patients were rehabilitated with complete mandibular overdenture supported by one-piece implants one on each side. Evaluation was made at the time of insertion, 6, 12, and 18 months after overdenture insertion, by measuring bacteriological changes around implants abutments. Complete overdenture supported by one-piece implants showed better effect on the bacteriological changes as compared to that supported by two-piece implants. Complete overdenture supported by one-piece implants one on each side of the lower arch showed better effect on the bacteriological changes than using the same prosthesis supported by two-piece implants.

  1. Impacts of cage culture on physico-chemical and bacteriological ...

    African Journals Online (AJOL)

    The effects of cage fish farming on physico-chemical and bacteriological water quality in Lake Volta, Ghana, were investigated in 2013–2014. Farmed ... The bacteriological analysis, however, revealed contamination of the lake water by fish farming. ... Keywords: aquaculture, contamination, fish farming, large lake, pollution ...

  2. Bacteriological Quality of Dried Sliced Beef (Kilishi) Sold In Ilorin ...

    African Journals Online (AJOL)

    DR. MIKE HORSFALL

    ABSTRACT: The bacteriological quality of dried sliced beef (kilishi) obtained from three selling points in. Ilorin metropolis was determined in order to ascertain its safety. The total bacterial count, Enterobacteriaceae count, Staphylococcus aureus count and E.coli counts were used as index of bacteriological quality. Samples.

  3. Physico-Chemical and Bacteriological Characteristics of Noyyal ...

    African Journals Online (AJOL)

    The study was conducted to findout the physico-chemical and bacteriological characteristics of Noyyal River and ground water quality of Perur, India. The river water and ground water (bore well and open well) samples were collected in and around Perur and analyzed for various physico-chemical and bacteriological ...

  4. Bacteriology laboratories and musculoskeletal tissue banks in Australia.

    Science.gov (United States)

    Varettas, Kerry

    2012-11-01

    In Australia, there are six Therapeutic Goods Administration-licensed clinical bacteriology laboratories providing bacterial and fungal bioburden testing of allograft musculoskeletal samples sent from 10 tissue banks. Musculoskeletal swab and/or tissue biopsy samples are collected at the time of allograft retrieval and sent to bacteriology laboratories for bioburden testing, in some cases requiring interstate transport. Bacteria and fungi may be present within the allograft at the time of retrieval or contaminated from an external source. The type of organism recovered will determine if the allograft is rejected for transplant, which may include all allografts from the same donor. Bacteriology staff also provides unpaid support of tissue banks through meeting involvement, consultations, licence-related activities, validations and research funded by their organisation and not part of any contractual agreement. Bacteriology laboratories and tissue banks must be compliant to the Code of Good Manufacturing Practice - Human Blood and Tissues and regulated by the Therapeutic Goods Administration. Clinical bacteriology laboratories also require mandatory accreditation to Standards Australia International Organisation for Standardisation (ISO) 15189:2009 medical laboratories - particular requirements for quality and competence, and may also attain Standards Australia/New Zealand Standard ISO 9001:2000 quality management systems certification. Bacteriology laboratories and musculoskeletal tissue banks are integral partners in providing safe allograft musculoskeletal tissue for transplant. © 2012 The Author. ANZ Journal of Surgery © 2012 Royal Australasian College of Surgeons.

  5. Impetigo—Bacteriologic Features and Renal Involvement

    Science.gov (United States)

    Shuler, Stanton E.

    1966-01-01

    One hundred children with impetigo were studied with particular emphasis upon the organism causing the infection and associated renal complications. In 50 per cent of cases, Group A beta-hemolytic streptococcus grew on cultures of material from the lesions, and evidence of recent infection with this organism as shown by an elevation of antistreptolysin O titer was present in an additional 17 per cent of cases. Acute glomerulonephritis developed in three of the 66 children with bacteriologic or serologic evidence of streptococcal infection. Four other children in this group and nine children with staphylococcal impetigo had unexplained microscopic hematuria. All children with nephritis already had evidence of the disease when first seen. In most of those with unexplained hematuria, this condition was detected at the first visit. Hematuria developed in others while they were receiving systemic antibiotics. The significance of isolated microscopic hematuria is uncertain, but is seen often in association with cutaneous infection with both staphylococcus and streptococcus. Microscopic hematuria as defined is apparently not prevented by antibiotic therapy. If acute glomerulonephritis that follows streptococcal cutaneous infection is to be prevented, streptococcal impetigo will have to be treated promptly after onset. PMID:5946549

  6. Bacteriological assessment of urban water sources in Khamis Mushait Governorate, southwestern Saudi Arabia

    Directory of Open Access Journals (Sweden)

    Sh AlOtaibi Eed L

    2009-03-01

    Full Text Available Abstract Background Urban water sources of Khamis Mushait Governorate, southwestern Saudi Arabia, were studied to assess their bacteriological characteristics and suitability for potable purposes. A cross-sectional epidemiological method was adopted to investigate the four main urban water sources (i.e. bottled, desalinated, surface, and well water. These were sampled and examined between February and June 2007. Results A total of 95 water samples from bottled, desalinated, surface, and well water were collected randomly from the study area using different gathering and analysing techniques. The bacteriological examination of water samples included the most probable number of presumptive coliforms, faecal coliforms, and faecal streptococci (MPN/100 ml. The results showed that the total coliform count (MPN/100 ml was not detected in any samples taken from bottled water, while it was detected in those taken from desalinated, surface, and well water: percentages were 12.9, 80.0, and 100.0, respectively. Faecal coliforms were detected in desalinated, surface, and well water, with percentages of 3.23, 60.0 and 87.88, respectively. About 6.45% of desalinated water, 53.33% of surface water, and 57.58% of well water was found positive for faecal streptococci. Colonies of coliforms were identified in different micro-organisms with various percentages. Conclusion Water derived from traditional sources (wells showed increases in most of the investigated bacteriological parameters, followed by surface water as compared to bottled or desalinated water. This may be attributed to the fact that well and surface water are at risk of contamination as indicated by the higher levels of most bacteriological parameters. Moreover, well water is exposed to point sources of pollution such as septic wells and domestic and farming effluents, as well as to soil with a high humus content. The lower bacteriological characteristics in samples from bottled water indicate that

  7. Bacteriological methods for distinguishing between human and animal faecal pollution of water: results of fieldwork in Nigeria and Zimbabwe

    OpenAIRE

    Mara, D. Duncan; Oragui, John

    1985-01-01

    Bacteriological techniques have traditionally been used to detect faecal pollution of drinking water supplies. Recently, methods have been developed to distinguish between human and animal faecal pollution in temperate climates. The present study assessed the applicability and practicality of these methods in tropical countries. Fieldwork in Nigeria and Zimbabwe has shown that animal faecal pollution can reliably be identified by the detection and enumeration of Rhodococcus coprophilus using ...

  8. effect of abattoir waste on the physicochemical and bacteriological

    African Journals Online (AJOL)

    HOD

    urbanization and the inadequate construction, operation and maintenance of ... EFFECT OF ABATTOIR WASTE ON THE PHYSICOCHEMICAL AND BACTERIOLOGICAL QUALITIES OF NEW-ARTISAN, … C. N. Eze & P. C. Eze. Nigerian ... liquid waste is usually composed of dissolved solids, blood, gut contents, urine and ...

  9. Bacteriologic and Histopathologic Studies in Pneumonic Lambs in ...

    African Journals Online (AJOL)

    Mortality in lambs is known to be caused by pneumonia and other bacterial agents. Lamb pneumonia is an infectious disease of young lambs caused primarily by the bacterial organism Pasterella haemolytica. Lung samples recovered from dead lambs were tested bacteriologically and histopathologically to determine the ...

  10. Physico-chemical and bacteriological quality of rainwater in Egbema ...

    African Journals Online (AJOL)

    Physico-chemical and bacteriological quality of rainwater in Egbema was determined with samples harvested directly, from zinc roof, thatched roof and asbestos roof, at different periods of the rainy season namely, Early, peak and late rains. The values of the physico-chemical parameters were on the higher side at the early ...

  11. Bacteriological examination of drinking water with reference to ...

    African Journals Online (AJOL)

    The study revealed that the number of coliforms was very high ( 1500) in water samples collected from mobile vendors. The bacteria were identified as Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Bacteriological examination of water samples collected from different sources showed that the ...

  12. Bacteriological examination of drinking water in Burdwan, India with ...

    African Journals Online (AJOL)

    SERVER

    2007-11-19

    Nov 19, 2007 ... Bacteriological examination of water samples collected from different sources showed that the water of mobile vendors and sweet shops of Burdwan market area was not potable while the municipal tap water was found to be safe for drinking. Key words: Drinking water, coliform, MPN test. INTRODUCTION.

  13. Constructing COMSOL Models of a Bacteriological Fuel Cell

    Science.gov (United States)

    Coker, Robert; Mansell, James

    2012-01-01

    We show very initial work on a specific bioelectrochemical system (BES), a bacteriologically driven 'fuel cell' (BFS), that is intended to process waste products, such as CO2 and brine. (1) Processing is the priority, not power generation (2) Really a Microbial Electrolysis Cell (MEC)

  14. bacteriological analysis of well water samples in sagamu.

    African Journals Online (AJOL)

    Dr Oboro VO

    diseases that affect human and animals cannot be underestimated. This is the most important concern about the quality of water. Guideline for bacteriological water ..... serious problem which calls for vigilance on the part of the authorities as it signals possible future outbreak of water borne diseases. Such disease outbreak ...

  15. Bacteriological quality of water samples in Osogbo Metropolis ...

    African Journals Online (AJOL)

    The bacteriological qualities of samples of some sachet water, tap water and well water were examined. Some physicochemical parameters (pH and suspended solids) indicative of water quality as well as the total bacterial and total coliform counts were examined. The pH of the samples range between 6.5 and 7.2.

  16. Bacteriology of Urine Specimens Obtained from Men with ...

    African Journals Online (AJOL)

    empiric use of antibiotics in this group of patients. KEYWORDS: Bacteriuria, benign prostatic hyperplasia, urinary tract infection. How to cite this article: Agbugui JO, Obarisiagbon EO, Osaigbovo II. Bacteriology of Urine Specimens Obtained from Men with Symptomatic. Benign Prostatic Hyperplasia. Niger J Surg 0;0:0.

  17. Bacteriology of the Anterior Genitalia of the Domestic House Cat ...

    African Journals Online (AJOL)

    The bacteriology of the anterior genitalia of the domestic house cat was determined using vaginal swabs collected from sixty apparently healthy female domestic cats (20 kittens, 20 pregnant and 20 non-pregnant adults). The swabs were streaked on blood agar, Mac Conkey agar and eosin methylene blue agar plates ...

  18. Effect of Boscia Albitrunca (Omukunzi) Root on the Bacteriology and ...

    African Journals Online (AJOL)

    The objective of this study was to determine the role of Omukunzi root (Boscia albitrunca) in the viscosity or consistency, sensory and bacteriological profile of Omashikwa, traditional fermented buttermilk from Namibia. Omashikwa is a popular traditional fermented buttermilk product made with Omukunzi root among the ...

  19. physico-chemical and bacteriological analyses of drinking water

    African Journals Online (AJOL)

    2012-07-02

    Jul 2, 2012 ... into an ice cube cooler and transported to the labora- tory for analysis of bacteriological and physicochem- ical parameters. In the laboratory, the samples were preserved by refrigeration and were analyzed within the confines of their respective “holding time”. 2.4. Method of data analysis. The underlisted ...

  20. Study of the bacteriological and physicochemical indicators of ...

    African Journals Online (AJOL)

    This study investigates the pollution level of surface waters in Zaria, Nigeria. The bacteriological and physicochemical analyses performed were in accordance with standard procedures. Out of 228 samples from different sites, 128 (56.1%) had counts higher than the standards. Samaru stream was the most polluted.

  1. Physico-Chemical and Bacteriological Analyses of Water Used for ...

    African Journals Online (AJOL)

    Samuel Olaleye

    Physicochemical and bacteriological analyses were carried out on well water, stream water and river water used for drinking and swimming purposes in. Abeokuta, Nigeria. The results obtained were compared with WHO and EPA standards for drinking and recreational water. With the exception of Sokori stream and a well ...

  2. Bacteriological and Physicochemical Quality of Drinking Water and ...

    African Journals Online (AJOL)

    BACKGROUND: Lack of safe drinking water, basic sanitation, and hygienic practices are associated with high morbidity and mortality from excreta related diseases. The aims of this study were to determine the bacteriological and physico-chemical quality of drinking water and investigate the hygiene and sanitation practices ...

  3. Bacteriological and physico-chemical quality of drinking water ...

    African Journals Online (AJOL)

    Background: Accesses to safe water is a universal need however, many of the world's population lack access to adequate and safe water. Consumption of water contaminated causes health risk to the public and the situation is serous in rural areas. Objectives: To assess the bacteriological and physico-chemical quality of ...

  4. Bacteriology of urinary tract infection and antimicrobial sensitivities ...

    African Journals Online (AJOL)

    Background: Urinary tract infection (UTI) is one of the serious bacterial infections in febrile young children, which may cause chronic morbidities. Studies from different parts of Nigeria have shown varying pattern in its bacteriology and antibiotic sensitivities. Antimicrobial resistance rate among uropathogens is an increasing ...

  5. Comparative study on the level of bacteriological contamination of ...

    African Journals Online (AJOL)

    Comparative study on the level of bacteriological contamination of automatic teller machines, public toilets and public transport commercial motorcycle crash ... Conclusion: This study has revealed the ability of these public devices to serve as vehicle of transmission of microorganisms with serious health implications.

  6. Bacteriological And Physico-Chemical Qualities Of Waste Water ...

    African Journals Online (AJOL)

    Waste water in the accumulation pond and final discharge point of Nigerian Bottling Company PLC in Owerri, Nigeria was analyzed to determine their bacteriological and physico-chemical characteristics. Species of organisms isolated included Staphyloccus, Bacillus, Lactobacillus, and Streptococcus. Others include ...

  7. Bacteriological and physicochemical analysis of waste water from ...

    African Journals Online (AJOL)

    Assessment of the bacteriological and physicochemical characteristics of ten freshwater fish ponds stocked with African catfish (Clarias gariepinus) was conducted within Ilorin metropolis. The results of physicochemical properties of the water samples showed that the pH, total hardness, salinity, and suspended solid ranged ...

  8. Assessment of physico-chemical and bacteriological quality of ...

    African Journals Online (AJOL)

    Water quality is a critical factor affecting human health and welfare. This study aimed at examining the physico-chemical and bacteriological quality of drinking water in Adama town. A total of 107 triplicate water samples were examined; 1 from inlet point (raw water), 1 from outlet (the water after treatment, 1 from reservoir ...

  9. Bacteriological Quality and Antibiogram of Isolates from Potable ...

    African Journals Online (AJOL)

    ADOWIE PERE

    ABSTRACT:This study sought to determine the bacteriological quality of some boreholes and storage tanks used for drinking and other domestic purposes in Ekosodin community and evaluated the antimicrobial susceptibility patterns of the groundwater resources isolates. A total of 48 samples were collected from three ...

  10. Observations on the Current Bacteriological Profile of Chronic ...

    African Journals Online (AJOL)

    Background: Chronic suppurative otitis media (CSOM) is a disease well‑known for its recurrence and persistence despite treatment. The situation is compounded by the increasing resistance to antimicrobial agents by bacteria these days. Aim: This study was carried out to examine the current local bacteriological profile of ...

  11. Pattern and bacteriology of acute suppurative otitis media in Sokoto ...

    African Journals Online (AJOL)

    Background: To describe the pattern of distribution, causative bacterial organisms, and management of acute suppurative otitis media in Sokoto, Nigeria. Method: A retrospective study of the bacteriology, clinical features and management of acute suppurative otitis media (ASOM) seen in a 2-year period. Results: Out of the ...

  12. Bacteriological analysis of spent engine oil contaminated soil ...

    African Journals Online (AJOL)

    The bacteriological analysis of soil contaminated with spent engine oil (SEO) planted with cowpea was investigated. The aim of this study was to detect the microbial degradation of SEO in soil and how it affects the microbial activity and the effects of SEO on the growth of cowpea. SEO collected from a mechanic workshop in ...

  13. Bacteriological Quality and Possible Health Effects of Gravity-Piped ...

    African Journals Online (AJOL)

    Ten years after the establishment of the GPWS, there were complains about the incidence of diarrhoea diseases, colouration of water and sediment in some of the systems put in place to provide potable water. The paper reports on bacteriological quality and health implications of water at various points (source, reservoir, ...

  14. Assessing the Bacteriological Quality of Drinking Water from ...

    African Journals Online (AJOL)

    Assessing the Bacteriological Quality of Drinking Water from Sources to Household Water Samples of the Rural Communities of Dire Dawa Administrative Council, Eastern Ethiopia. ... This is due to lack of good water treatment, improper water handling practices and lack of the protection of the water sources. Consequently ...

  15. Bacteriology of post–operative wound infections in the surgical ...

    African Journals Online (AJOL)

    A total of 248 swab specimens from post-operative wounds suspected to be infected were bacteriologically evaluated. Of these, bacterial growth was recorded in 204 specimens (82.3%) while 44 specimens (17.7%) yielded no growth. The bacteria isolated from the samples in decreasing order of prevalence were: ...

  16. Bacteriological pollution indicators in Ogun River flowing through ...

    African Journals Online (AJOL)

    Water resources are significant part of integrated community development policy and good health. Hence, the need to reduce the impact of natural and anthropogenic pollution causes so as to enhance water quality. The bacteriological quality of the Ogun River was investigated to determine the sanitary conditions of the ...

  17. The effect of storage on Physical, Chemical and Bacteriological ...

    African Journals Online (AJOL)

    This study assessed the changes in physical, chemical and bacteriological quality of drinking water stored for a period of three months. Ten (10) different companies' water samples each of bottled water (B) and sachet water (S) were randomly selected for the study around Ibadan Metropolis. Experimental method was used ...

  18. Bacteriology of urine specimens obtained from men with ...

    African Journals Online (AJOL)

    Background: Bacteriuria and urinary tract infections are common sequelae of benign prostatic hyperplasia (BPH). Thus, the knowledge of urine bacteriology in men with symptomatic BPH in our environment may play a complementary role in management. Objectives: To determine the incidence of bacteriuria and the ...

  19. Bacteriological profile in neonatal sepsis at the University teaching ...

    African Journals Online (AJOL)

    Infections are the biggest cause of neonatal deaths in developing countries and most of them can be preventable. Predominant causative bacterial agents kept changing overtime as such, this retrospective cross sectional study was designed to determine the bacteriological profile in neonatal sepsis and their susceptibilty to ...

  20. [Method and procedures in bacteriological study of necrotic teeth].

    Science.gov (United States)

    Rodríguez-Ponce, A; López Campos, A; López Paz, J; Pazos Sierra, R

    1991-01-01

    Research was conducted of 160 radicular canals with necrotic pulp. Results of different bacteriological analyses are presented. Culture analyses in aerobic and anaerobic media, resulted in the isolation of Staphylococcus Epidermidis, Streptococcus Viridans and Corynebacterium sp in the group studied, as the most frequent bacteria. There was no evidence of a specific germ linked with the pulp necrosis.

  1. Bacteriological and Physcio – Chemical Evaluation of Water Treated ...

    African Journals Online (AJOL)

    Bacteriological and physico-chemical evaluation of water coagulated with Moringa oleifera seed powder preparations on Challawa river water intake station was conducted for a period of ... The implications of the results are discussed in relation to water sanitation and human health and some recommendations presented.

  2. Observations on the Current Bacteriological Profile of Chronic ...

    African Journals Online (AJOL)

    Background: Chronic suppurative otitis media (CSOM) is a disease well‑known for its recurrence and persistence despite ... Orji and Dike: Anerobic bacteriological profile of chronic suppurative otitis media. Annals of Medical and Health ... Fifty-two percent were children aged 5 months to 15 years. Those aged <5 years ...

  3. physico-chemical and bacteriological analyses of drinking water

    African Journals Online (AJOL)

    2012-07-02

    Jul 2, 2012 ... Physicochemical, heavy metals and bacteriological analysis of drinking water samples from ten. (10) geo-referenced points in five ... Analysis of Drinking Water in Ibeno LGA Akwa Ibom State. 117 ment Area is one of the coastal .... using mercury-in-glass broth thermometer. Water pH was taken in-situ using ...

  4. Physico-chemical and bacteriological analyses of water used for ...

    African Journals Online (AJOL)

    Physicochemical and bacteriological analyses were carried out on well water, stream water and river water used for drinking and swimming purposes in. Abeokuta, Nigeria. The results obtained were compared with WHO and EPA standards for drinking and recreational water. With the exception of Sokori stream and a well ...

  5. Bacteriological Survey of the Frozen Prepared Foods Industry

    Science.gov (United States)

    Surkiewicz, Bernard F.; Groomes, Ralph J.; Padron, Adriano P.

    1967-01-01

    During sanitation-inspections of 29 potato-processing firms, 2,544 finished product units and 1,654 samples were collected and analyzed bacteriologically. The results of the bacteriological examination of finished French fries, fried potato cylinders, and dehydrated potatoes usually did not reveal the conditions of cleanliness under which they were produced. This was most likely due to the lethal effect of the terminal fry to which the French fries and potato cylinders are subjected, and because of the lethal effect of the dehydration temperatures used in processing the dehydrated potato products. However, as in most food-processing firms, line samples collected at each processing step reflect sanitary conditions and provide bacteriological support of inspectional evidence of plant insanitation. Most of the frozen stuffed baked potatoes examined were produced under poor sanitary conditions. But because this product is usually processed while hot, bacteriological examination of the finished product did not usually reveal the conditions of cleanliness under which they were produced. Again, inspectional observations were necessary for a full evaluation of the conditions of production. In contrast, frozen potato patties and frozen hash brown potatoes were more likely to reveal the conditions of sanitation under which they were produced as evidenced by the varying bacterial counts. PMID:16349740

  6. Physiochemical and Bacteriological Analysis of Selected Sachet Water in Jere and Maiduguri, Borno State Nigeria

    Directory of Open Access Journals (Sweden)

    Mohammed Modu Aji

    2015-02-01

    Full Text Available  The study involved the determination of some physiochemical and bacteriological properties in sachet-water samples taken within selected geopolitical wards in Jere and Maiduguri Metropolis. The purpose was to ascertain the quality of sachet water sold for consumption within the area. Ten samples were drawn, five from each study area. The samples were analysed for temperature, colour, turbidity, pH, conductivity, iron, total alkalinity and total dissolved solid. Bacteriological analysis was also carried out using multiple tube (most probable number technique for enumeration of both total coli form count and differential Escherichia coli count. Other physical examination like volume, National Agency for Food and Drugs Administration and Control (NAFDAC registration number, batch number, production date and expiry date were also examined. The results obtained were compared with World Health Organization (WHO, NAFDAC and Nigeria Standard of Drinking Water Quality (NSDWQ. Variations were observed among the entire samples in comparison with the standard. Some of the parameters conform to the standard like the Tempreture, pH, colour, odour, iron content and NAFDAC registration number while others like TDS, total alkalinity and volumetric quantity fell below the standard. The bacteriological analysis also showed that 80% of the samples studied revealed the presence of coliforms. Hence, there is need for regulatory agencies like NAFDAC and Standard Organisation of Nigeria (SON to intensify effort in the routine monitoring of quality of sachet water marketed for consumption.DOI: http://dx.doi.org/10.3126/ije.v4i1.12177 International Journal of Environment Volume-4, Issue-1, Dec-Feb 2014/15, page: 48-57  

  7. Clinical and bacteriological study of the effect of Nd:YAG laser in gingivitis therapy

    Science.gov (United States)

    Colojoara, Carmen; Mavrantoni, Androniki; Miron, Mariana I.

    2000-06-01

    The relationship between dental plaque and gingivitis was verified. Nonspecific gingivitis is an inflammatory process, frequently caused by enzymes and toxins liberate by bacteria form dental plaque. Loose plaque has come under a great deal of investigation because of its role in attachment loss. The current methods used in the treatment of non specific gingivitis encompass the use of antibiotics and conventional surgical techniques. Treating gingivitis with laser energy may further reduce the gingival inflammation and decrease the wound healing time. The lack of correlation between the quantity of dental plaque and the intensity of gingivitis determined us to study the effect of Nd:YAG pulsed laser in reduction of gingival inflammation and wound healing. The aim of this work is to evaluate clinically the anti- inflammatory and wound healing effect of pulsed Nd:YAG laser and to compare the appearance and the levels of the bacteria in the supergingival and subgingival plaque in adolescents with tooth crowding after Nd:YAG laser. The experimental procedure consisted of a clinical and bacteriological study which was undertaken in 20 patients presenting moderate gingivitis. A group of 10 patients was the subject of a bacteriological study and the other group of 10 was used for clinical and histological examination. For each group the clinical criteria of evaluation were: the gingival index, papillary bleeding index, spontaneous aches. Each patient was tested before and after laser exposure or conventional therapy for bacteriological analyses. The results prove that early gingivitis exposure to laser registers a decrease of bacterial colony number and absence of loss of attachment as compared to the application of the conventional treatment. Clinical study has shown that the combination of scaling and root planning with laser therapy is enough to provide improvement in clinical indices and reduction in the number of bacterial colonies.

  8. Bacteriological analysis of Periplaneta americana L. (Dictyoptera ...

    African Journals Online (AJOL)

    SERVER

    2007-09-05

    Sep 5, 2007 ... Center of Mediterranean and Environmental Studies, Laboratory of Applied Biology and Environment Sciences,. Department of Life sciences, Faculty of Sciences and Techniques, Abdelmalek Essaâdi University, Tangier, BP 416,. Morocco. Accepted 23 April, 2007. In this study, Periplaneta americana and ...

  9. Bacteriological and parasitological evidence of soil contamination ...

    African Journals Online (AJOL)

    Domestic environmental pollution resulting from human's cohabitation with domestic and farm animals were investigated in. Makurdi using multiple tubes fermentation for identification of faecal indicator bacteria and test tube floatation techniques for the parasitological analysis of soil samples respectively. Of the 150 soil ...

  10. Bacteriological diagnosis and molecular strain typing of Mycobacterium bovis and Mycobacterium caprae.

    Science.gov (United States)

    Gormley, E; Corner, L A L; Costello, E; Rodriguez-Campos, S

    2014-10-01

    The primary isolation of a Mycobacterium sp. of the Mycobacterium tuberculosis complex from an infected animal provides a definitive diagnosis of tuberculosis. However, as Mycobacterium bovis and Mycobacterium caprae are difficult to isolate, particularly for animals in the early stages of disease, success is dependent on the optimal performance of all aspects of the bacteriological process, from the initial choice of tissue samples at post-mortem examination or clinical samples, to the type of media and conditions used to cultivate the microorganism. Each step has its own performance characteristics, which can contribute to sensitivity and specificity of the procedure, and may need to be optimized in order to achieve the gold standard diagnosis. Having isolated the slow-growing mycobacteria, species identification and fine resolution strain typing are keys to understanding the epidemiology of the disease and to devise strategies to limit transmission of infection. New technologies have emerged that can now even discriminate different isolates from the same animal. In this review we highlight the key factors that contribute to the accuracy of bacteriological diagnosis of M. bovis and M. caprae, and describe the development of advanced genotyping techniques that are increasingly used in diagnostic laboratories for the purpose of supporting detailed epidemiological investigations. Copyright © 2014 Elsevier Ltd. All rights reserved.

  11. [Verification of bacteriological safety of PCM 40 air conditioner].

    Science.gov (United States)

    Dumas, J L; Ducel, G; Rouge, J C

    1991-01-01

    This study assessed the bacteriological safety of the bedside air conditioner PCM 40 (Howorth Airtech), used for prevention of intraoperative hypothermia, by blowing filtered warm air through a special mattress. The 3 microns bacterial filter of the device released 2,968 +/- 5,618 particles of diameter less than 3 microns per m3 of room air, containing 78,798 +/- 37,243 of such particles per m3. The amount of bacteries in the air pulsed from the mattress was 30 +/- 41 cfu/m3 vs 120 cfu/m3 in the ambient air and in the hot air supply tubing it reached 6 +/- 5 cfu/m3 vs 175 +/- 77 cfu/m3. It is concluded that bacteriological data do not contra-indicate the use of this air conditioner in the operating theater. The only limitations for use are the position (prone or lateral position) and type of surgery (neurosurgery).

  12. [Perinatal mortality in dogs. Clinical, bacteriological and pathological studies].

    Science.gov (United States)

    Sager, M; Remmers, C

    1990-08-01

    1. In intensively operated dog breeding kennels bacterial infections are very significant in perinatal mortality. 2. Staph. aureus, Streptococci (type G) and also beta-haemolytic E. coli were transmitted intra-uterine or by the infected genital tract to the puppies. In many cases they are the cause of septicaemic death of the puppies. 3. A second important cause of infection is subclinical mastitis of the bitch, leading to septicaemic death of newborn puppies. 4. Prophylactic hygienic measures make possible a prognosis concerning the risk of perinatal death. This includes examinations of the dog and the bitch ante coitum, bacteriological examination of the genital tract of the bitch, and a bacteriological examination of the milk before the date of birth. 5. Prophylactic hygienic measures in combination with antibiotic treatment of the bitch or the puppies could reduce the losses of puppies to less than 10%.

  13. Bacteriological analysis of borehole water in Uli, Nigeria | Ibe ...

    African Journals Online (AJOL)

    Water samples from four different boreholes within Uli, Anambra State, Nigeria, were collected for five consecutive weeks for bacteriological analysis to assess the potability.The range of the means was from 1.5x102 to 5.9x104 cfu ml-1 for total aerobic bacterial load, 9 to 136 MPN per 100ml for total coliforms and 4 to 74 ...

  14. [Study of the bacteriological quality of water used in the agro-food industry in the North of Morocco].

    Science.gov (United States)

    Haijoubi, El Houcine; Benyahya, Fatiha; Bendahou, Abdrezzak; Essadqui, Faima Zahra; Behhari, Mohammed El; El Mamoune, Ahmed Fouad; Ghailani, Naima Nourouti; Mechita, Mohcine Bennani; Barakat, Amina

    2017-01-01

    Water is used predominantly in food manufacturing process. Northern morocco agro-food industries use different sources of water, but public water and wells water are the main sources of water used. This water can be the main source of possible food contaminations and alterations. This study aims is to assess the bacteriological quality of water used in the agro-food industries in the Northern region of Morocco, to identify the different types of germs responsible for the pollution of these waters and to establish the main causes of this pollution. Water samples taken from taps or wells were analyzed to detect pollution indicator germs (total coliform (TC), fecal coliform (FC), intestinal enterococci (E), revivable microorganisms (RM), sulphite-reducing anaerobes) and pathogens (Salmonella, Staphylococci, Pseudomonas aeruginosa). The enumeration of the bacteria was performed by filtration technique and incorporation obtained through supercooled solid state. The results showed that public-supply waters were of satisfactory bacteriological quality while 40% of the wells water was non-compliant with water quality standards due to the presence of TC, FC, E and RM pollution indicators. In contrast, pathogens, particularly Salmonellae, were absent in all the wells water analyzed. Well water pollution was generally due to failure to meet hygienic requirements for water pumping. Bacteriological quality of these wells water could be improved by adequate protection.

  15. Aerobic bacteriology of burn wound infections

    Directory of Open Access Journals (Sweden)

    Sarita Otta

    2015-01-01

    Full Text Available Introduction: Burn wound infections are important cause of mortality, morbidity and prolonged hospitalization in burn patients as the causative agent is generally a multidrug resistant organism. The pattern of microbial flora infecting burn wound varies according to geographical pattern as well as with duration of hospital stay. Objective: The present study aims to identify the causative agents of burn wound infections in our hospital, to assess the change in pattern of flora in accordance to duration of wound as well as to determine the sensitivity pattern of isolates. Materials and Methods: Wound swabs from 52 patients admitted in Burn unit of IMS and SUM Hospital, Bhubaneswar were collected every 5th day during their hospital stays and were cultured. The results were interpreted according to the standard methods and antibiotic susceptibility pattern was noted. Results: The most common organism isolated was Staphylococcus aureus, that is, 20 (47.6% while Klebsiella spp. 12 (28.6% was the most common Gram-negative isolate. The commonest organism isolated in the 1st week of hospital stay was S. aureus (15.4%, but it was Acinetobacter spp. (22.2% in 2nd week and coagulase negative Staphylococcus in 3rd week of hospital stay S. aureus was mostly sensitive to levofloxacin (77.8% and netilmycin (98%. Conclusion: The pattern of bacterial flora changes according to duration of hospital stay. Effective strict isolation techniques and infection control are thus needed to decrease the occurrence of burn wound infection.

  16. Tercer Simposio Internacional de Bacteriología

    Directory of Open Access Journals (Sweden)

    Ana Elvira Farfán-García

    2016-06-01

    Full Text Available Este suplemento reúne 24 resúmenes de proyectos de investigación básica y aplicada que fueron presentados en el marco del Tercer Simposio Internacional de Bacteriología, organizado por el programa de Bacteriología y Laboratorio Clínico de la Facultad de Ciencias de la Salud de la Universidad de Santander UDES, sede Bucaramanga entre el 27 al 29 de abril de 2016 en el marco de la celebración de los 20 años de funcionamiento del programa. En este evento se reunieron investigadores nacionales, internacionales, profesores, estudiantes de pregrado y posgrado, quienes compartieron experiencias y avances de investigación en torno a las ciencias biomédicas. Dentro de los objetivos del tercer simposio estaba divulgar los resultados de investigación de investigadores y estudiantes de diferentes instituciones regionales y nacionales así como establecer alianzas y acercamientos con científicos e investigadores de las instituciones participantes para el fortalecimiento de las líneas de investigación del Grupo de Investigación en Manejo Clínico –CliniUDES-. Entre los temas que fueron tratados en el simposio se destacan: enfermedades emergentes y cambio climático, enfermedades virales tropicales, hematología clínica, síndrome metabólico, actualización en diagnóstico de diabetes, cáncer y marcadores tumorales, gestión del riesgo y genética forense. Los organizadores del evento fueron la Dra. Juanita Trejos Suárez y el Dr. Sergio Yebrail Gómez Rangel acompañados por un comité científico y académico y por estudiantes del programa de Bacteriología y Laboratorio Clínico.

  17. Bacteriological and mycological surveys along Otranto coastal areas

    International Nuclear Information System (INIS)

    Montagna, M.T.; De Donno, A.; Bagordo, F.; Carrozzini, F.

    2000-01-01

    Bacteriological and mycological surveys were carried out on water samples collected from 6 seaside resorts and 2 affluent along Otranto coastal areas. The following parameters were tested: total coliforms, fecal coliforms, fecal streptococci, Salmonella, P. aeruginosa and S. aureus, Vibrio species and yeasts. Results show fecal pollution in three seaside resorts and in one drain, whereas Salmonella research always resulted negative. P. aeruginosa and S. aureus were isolated from both affluents. V. alginolyticus was the most isolated vibrio specie (90%). Among isolated yeasts Torulopsis inconspicua (70%) was the most frequent one [it

  18. Improvement of bacteriological quality of frozen chicken by gamma radiation

    International Nuclear Information System (INIS)

    Nouchpramool, K.; Prachasitthisak, Y.; Charoen, S.; Kanarat, S.; Kanignunta, K.; Tangwongsupang, S.

    1986-12-01

    The possible use of gamma irradiation at doses of 1.6 to 4.0 kGy to improve bacteriological quality of frozen chicken was investigated. The effects of gamma irradiation on salmonella viability in frozen chicken and on sensory quality of frozen chicken were also evaluated. D 10 -values for different isolated strains of salmonella in frozen chicken varied from 0.41 to 0.57 kGy. A dose of 4 kGy is required for a seven log cycle reduction of salmonella contamination in frozen chicken. Approximately 21 per cent of frozen chicken examined were contaminated with salmonella. Salmonella typhimurium, salmonella virchow, and salmonella java were predominant. Irradiation of frozen chicken at a minimum dose of 3.2 kGy eliminated salmonella, coliform, Escherichia coli, and Staphylococcus aureus and, in addition, reduced baterial load by 2 log cycles. Faecal streptococci was still present in a 3.2 kGy samples but in a very small percentage and the count was not over 100 colonies per g. Discoloring of chicken meat was noted after a 2 kGy treatment. The sensory quality of frozen chicken irradiated at 3 and 4 kGy tended to decrease during frozen storage but was within the acceptable range on a nine point hedonic scale even after eight months of frozen storage. Dosage at 3.2 kGy appeared to be sufficient for improving bacteriological quality of frozen chicken

  19. Laboratory automation in clinical bacteriology: what system to choose?

    Science.gov (United States)

    Croxatto, A; Prod'hom, G; Faverjon, F; Rochais, Y; Greub, G

    2016-03-01

    Automation was introduced many years ago in several diagnostic disciplines such as chemistry, haematology and molecular biology. The first laboratory automation system for clinical bacteriology was released in 2006, and it rapidly proved its value by increasing productivity, allowing a continuous increase in sample volumes despite limited budgets and personnel shortages. Today, two major manufacturers, BD Kiestra and Copan, are commercializing partial or complete laboratory automation systems for bacteriology. The laboratory automation systems are rapidly evolving to provide improved hardware and software solutions to optimize laboratory efficiency. However, the complex parameters of the laboratory and automation systems must be considered to determine the best system for each given laboratory. We address several topics on laboratory automation that may help clinical bacteriologists to understand the particularities and operative modalities of the different systems. We present (a) a comparison of the engineering and technical features of the various elements composing the two different automated systems currently available, (b) the system workflows of partial and complete laboratory automation, which define the basis for laboratory reorganization required to optimize system efficiency, (c) the concept of digital imaging and telebacteriology, (d) the connectivity of laboratory automation to the laboratory information system, (e) the general advantages and disadvantages as well as the expected impacts provided by laboratory automation and (f) the laboratory data required to conduct a workflow assessment to determine the best configuration of an automated system for the laboratory activities and specificities. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

  20. Assessment of the relationship between bacteriological quality of dug-wells, hygiene behaviour and well characteristics in two cholera endemic localities in Douala, Cameroon.

    Science.gov (United States)

    Akoachere, Jane-Francis Tatah Kihla; Omam, Lundi-Anne; Massalla, Thomas Njinuwo

    2013-07-29

    Access to potable water is grossly inadequate in Douala-Cameroon. The situation is worse in slum areas, compelling inhabitants to obtain water from sources of doubtful quality. This has contributed to frequent outbreaks of water-borne diseases particularly cholera, which results in severe morbidity and mortality. Shallow wells are a major source of water in these areas. We analyzed the influence of some factors on the bacteriological quality of well water in Bepanda and New Bell, cholera endemic localities in Douala to generate data that would serve as basis for strengthening of water and health policies. Questionnaires were administered to inhabitants of study sites to appraise their hygiene and sanitation practices, and level of awareness of waterborne diseases. The bacteriological quality of water was determined by investigating bacterial indicators of water quality. Relationship between well characteristics and bacteriological quality of water was determined using χ² test. The Kendall tau_b nonparametric correlation was used to measure the strength of association between well characteristics and bacteriological parameters. Statistics were discussed at 95% confidence level. Antibiotic susceptibility of isolates was investigated by the Kirby-Bauer and broth dilution techniques. Multidrug resistant species were tested for extended β-lactamase production potential. Inhabitants demonstrated adequate knowledge of waterborne diseases but employed inappropriate method (table salt) for well disinfection. Well construction and location violated guidelines. Indicator bacterial counts greatly exceeded the WHO guidelines. Variation in bacteriologic parameters between sites was not significant (P > 0.05) since well characteristics and hygiene and sanitary practices were similar. Differences in bacteriologic quality with respect to state of well, and presence of molded casing and lid, and height of casing were not significant (P > 0.05). Well distance from sanitary

  1. 9 CFR 147.11 - Laboratory procedure recommended for the bacteriological examination of salmonella.

    Science.gov (United States)

    2010-01-01

    ... the bacteriological examination of salmonella. 147.11 Section 147.11 Animals and Animal Products... procedure recommended for the bacteriological examination of salmonella. (a) For egg- and meat-type chickens... 25 birds, and birds from Salmonella enteritidis (SE) positive environments should be cultured in...

  2. Invisible enemies: bacteriology and the language of politics in imperial Germany.

    Science.gov (United States)

    Gradmann, C

    2000-01-01

    The text analyzes the related semantics of bacteriology and politics in imperial Germany. The rapid success of bacteriology in the 1880s and 1890s was due not least to the fact that scientific concepts of bacteria as "the smallest but most dangerous enemies of mankind" (R. Koch) resonated with contemporary ideas about political enemies. Bacteriological hygiene was expected to provide answers to social and political problems. At the same time metaphors borrowed from bacteriological terminology were incorporated into the political language of the time. While the "high command of our doctors" (F.J. Cohn) fought diseases, some contemporaries were identified with members of the evil species of "bacillus communis odii." Both imperialistic politics and bacteriological science relied on images of inferior and invisible but potent enemies. Both were able to increase their prestige via a mutual interchange of their vocabularies.

  3. Changing bacteriological profile and mortality trends in community acquired pneumonia

    Directory of Open Access Journals (Sweden)

    Sagar Khadanga

    2014-01-01

    Full Text Available There are very few and conflicting Indian data regarding the bacteriological etiology of community acquired pneumonia (CAP. Adding to this agony, there is no credible data from the eastern part of India. This is a cross-sectional study and descriptive in nature over a period of 1-year. Of the 464 cases of the study population, we could isolate aerobic bacteria in 149 patients (32.1%. Streptococcus pneumoniae has been identified as the most common organism causing CAP (68/149. Gram-negative bacilli (GNB as a group exceeded marginally over S. pneumoniae (69/149. Among GNB, Pseudomonas aeruginosa was the most common organism (31/69, followed by Klebsiella pneumoniae (29/69. Staphylococcus aureus was identified in (12/149 cases. Co-amoxyclav is still the most sensitive drug for S. pneumoniae. P. aeruginosa was most sensitive to imipenam followed by piperacillin-tazobactam.

  4. [Value of bacteriologic studies within the scope of forensic autopsies].

    Science.gov (United States)

    Schneider, V

    1985-01-01

    The various questions regarding bacteriological investigations within the scope of forensic autopsies are discussed in the light of our own cases and with regard to the relevant literature: estimate of the age of the corpse on the basis of decomposition changes caused by bacteria; determination of so-called bacteriograms from the point of view of criminalistics; supplementary investigations of the cause of death. Iatrogenic infections (e.g., gangrene) are gone into in more detail, as is so-called infantile botulism within the scope of the "sudden infant death syndrome". Finally, it is pointed out that when doing forensic autopsies, thought should also be given to illnesses that have only been known for a few years, examples being legionnaires' disease and the toxic shock syndrome ("tampon sickness").

  5. Radiometric determination of the bacteriological quality of raw milk

    International Nuclear Information System (INIS)

    Cogan, T.M.; O'Connor, F.

    1977-01-01

    The usefulness of 14 CO 2 production from (U- 14 C) glucose, (U- 14 C) glutamate and ( 14 C) formate as an index of raw milk quality was investigated. Development work showed that the volume of milk sample influenced the detection of radioactivity. The regression coefficient between Log 10 of the inital bacterial count and the detection time (the time to reach 2000 cpm) in the presence of 0.13 μCi (U- 14 C) glucose/ml was -0.55 (n=119 samples) and the relationship was y=-0.11x+-0.46. The addition of 0.5 μCi (U- 14 C) glutamate and 0.5 μCi of ( 14 C) formate decreased the detection times but did not result in greater predictability. Further study is required before the method could be used to determine the bacteriological quality of milk

  6. AERO-BACTERIOLOGICAL STUDY OF VEGETABLES MARKET AT JABALPUR

    Directory of Open Access Journals (Sweden)

    A. K. Pathak ، K. S. Verma

    2009-07-01

    Full Text Available Urban and rural vegetable markets of India characterized by mass activity with a little sanitary measure are responsible for generation of higher quantity of aerosols containing biotic and abiotic components. The aerosols generated in due course of mechanical disturbance, contain many organic compounds enable to sensitize vital respiratory organs of local inhabitants. Inhalation of airborne microorganisms can expose workers to risks from infection, toxicosis and allergy. The presence of non-spore forming gram-negative bacteria in air due to the mechanical areosolization indicates higher rate of dissemination of pollutants in these occupation associated areas. The present aero-bacteriological investigation included enumeration, identification and numerical analysis of different types of culturable airborne bacteria with inhalable fraction of gram-negative bacteria in the vegetable market of the city of Jabalpur, in order to measure the degree of aerobiopollution for this environment. The aero-bacteriological sampling has been done fortnightly for a period of one year. Samples were cultured based on standard methods. The survey revealed that in this type of atmosphere, environmental factors were responsible for the persistence of airborne bacteria with variable effects. The major contributors for aerosol generation were other mechanical activities, since this site is classified under human activity-enriched and highly trafficked site. The bioload of this atmosphere was recorded as high as 2.9 x 103 bacterial carrying particles per cubic meter during winter, dominated by both inhalable and non inhalable fractions of gram-negative bacteria. In summer, soil-borne bacteria were reported dominant in the air. High humidity and low temperature were the major factors for dissemination and distribution of gram-negative bacilli. A regression model with upto 43% variance was prepared in order to predict the bioload for this atmosphere in relation to

  7. The bacteriology of diabetic foot ulcers in Port Harcourt, Nigeria.

    Science.gov (United States)

    Unachukwu, C N; Obunge, O K; Odia, O J

    2005-01-01

    Diabetic foot ulcer and/or gangrene is a common cause of morbidity and mortality in Nigeria. The lesions are usually infected and early treatment of the infection will reduce the associated problems. The study was carried out to determine the common bacteriological flora of diabetic foot ulcers in Port Harcourt. The antimicrobial sensitivity pattern of the isolates was determined to enhance possible empirical treatment. Deep wound swabs were collected from 60 consecutive diabetic patients admitted with foot ulcers and/or gangrene into the medical wards of the University of Port Harcourt Teaching Hospital from January 2001 to April 2002. The bacteriological isolation and antimicrobial sensitivity tests of the isolates was carried out by standard microbiological methods. Aerobes and anaerobes constituted 95.4% and 4.6% of the total bacterial isolates respectively. Staphylococcus aureus was the commonest bacterial isolate; it was cultured from 32 (56.1%) of infected patients and constituted 24.4% of the total isolate. The mean bacterial isolate per patient infected was 2.3. The aerobic isolates showed significant sensitivity to ciprofloxacin (78.4%), pefloxacine (71.2%), ceftazidime (73.6%) and cefuroxime (69.6%). All the anaerobic isolates were sensitive to metronidazole and clindamycin. Infections of diabetic foot ulcers are usually polymicrobial. From the in vitro antimicrobial susceptibility pattern of the bacterial isolates, diabetic patients presenting with foot ulcers and/or gangrene could be commenced empirically on a combination of clindamycin or metronidazole and either a fluoroquinolone (ciprofloxacin or pefloxacine) or a second or third generation cephalosporin (e.g. cefuroxime or ceftazidime).

  8. The environmental influences on the bacteriological quality of red and chicken meat stored in fridges

    Directory of Open Access Journals (Sweden)

    Hossein Masoumbeigi

    2017-04-01

    Conclusions: The results implied that the bacteriological quality of red and chicken meat fluctuates with environmental status (especially temperature, sanitary status and personal hygiene. Regular control, improving of sanitary health, and staff training are necessary for elimination of bacterial contamination.

  9. Water quality and bacteriology in an aquaculture facility equipped with a new aeration system

    Digital Repository Service at National Institute of Oceanography (India)

    Fernandes, S.O.; Kulkarni, S.S.; Shirodkar, R.R.; Karekar, S.V.; PraveenKumar, R.; Sreepada, R.A.; Vogelsang, C.; LokaBharathi, P.A.

    The HOBAS aeration system was tested to compare changes in environmental and bacteriological parameters in ponds growing Penaeus monodon during a single production cycle. The stocking density in the aerated pond was doubled to 12 post-larvae (PL) m...

  10. Page 1 Bacteriological Analysis of Protected Springs Worku L, et al ...

    African Journals Online (AJOL)

    % water supply system, and a bacteriological coverage (5) indicating that the decade's .... Gray NF. Drinking water quality each spring for potential contamination has problems and solutions. John Wiley & shown almost all springs sampled lack.

  11. Bacteriological profile and antibiotic sensitivity pattern in patients with Urinary tract infection

    OpenAIRE

    N Subedi; S Pudasaini

    2017-01-01

    Background: Urinary tract infection is one of the common bacterial infections seeking treatment in clinical practice. A variety of organisms are associated with UTI and the most common organisms are Escherichia coli and other coliforms. Bacteriological investigations of UTI are not complete without antibiotic sensitivity test of the isolate. The aim of this study is to determine the bacteriological profile and antibiotic sensitivity patterns and their disease association.Materials and methods...

  12. Bacteriological research for the contamination of digital portable radiography

    Energy Technology Data Exchange (ETDEWEB)

    Shin, Seong Gyu [Dept. of Radiology, Dong A University Hospital, Busan (Korea, Republic of); Lee, Hyo Yeong [Dept. of Radiological Science, Dong Eui University, Busan (Korea, Republic of)

    2017-03-15

    The study was performed to investigate the bacteriological contamination of portable digital radiography system and their detectors in a university hospital. CNS and VRE were detected in the samples collected from vinyl cover on detectors used for the infection control patients. On the other hand, no bacteria was detected in the samples collected from detectors with vinyl cover removed. In the series of imaging of patients from general wards, no bacteria was detected from the patient 1. However, CNS was detected from the patients 2 and 3, CNS and Enterococcus faecalis detected from the patient 4, CNS and Enterococcus casseliflavus detected from the patient 5, and CNS, Enterococcus casseliflavus, and Klebsiella pneumoniae all detected from the patient 6. CNS and Enterococcus faecium were detected in the controller handle of collimator. Also, CNS was detected from the handle of detector and exposure switch. In the treatment gloves of the radiological technologist after the imaging, CNS, Enterococcus gallinarum, and Klebsiella pneumoniae were detected. Therefore, it is recommended for DR portable to take images after sterilizing the detector after taking each image and to use disposable vinyl covers on detectors to remove after imaging. And treatment gloves must be changed after each imaging. Also, hospital infection via portables must be prevented by complete sterilization of the controller handles of collimator which are in frequent contact during imaging and infection education of employees.

  13. Bacteriological quality of icecream marketed in Thrissur town, Kerala, India

    Directory of Open Access Journals (Sweden)

    R Ambily

    Full Text Available Aim: To assess the bacteriological quality of ice creams marketed in Thrissur town, Kerala. Materials and Methods: Twenty seven ice cream samples randomly collected from selected retail outlets and street vendors in the town over a period of two months were analyzed for total viable count and for the presence of coliforms, Escherichia coli, Staphylococcus aureus, Salmonella spp. and Listeria monocytogenes. Results: Total Viable Count ranged from 1.2 x 10 2 to 8.2 x 10 3 CFU/g in industrial brands whereas in street vendor samples it was high in the range of 5.2 x 10 5 to 6.6 x 10 6 CFU/g. Coliform counts were high in 30 % of the samples. 27 % of the samples had high Staphylococcal count. E coli were isolated from two samples from street vendors and Salmonella spp. from one of the samples. Listeria spp. was not isolated from any of the samples. Conclusion: The presence of potential pathogens in the ice cream samples reveals the significance of implementation of quality control measures in marketing ice creams thus reducing the public health hazards. [Vet World 2012; 5(12.000: 738-741

  14. Bacteriological Profile of Blood Culture Positive Sepsis in Newborn at BPKIHS, Dharan Nepal

    Directory of Open Access Journals (Sweden)

    Piush Kanodia

    2017-03-01

    Full Text Available Background & Objectives: Neonatal infections currently cause about 1.6 million deaths annually in developing countries. Sepsis and meningitis is responsible for most of these deaths. This study was undertaken to determine the bacteriological profiles and antibiotic sensitivity patterns of isolates from blood cultures of neonates admitted in a tertiary care hospital in Eastern Nepal.Materials & Methods: A retrospective study was conducted at pediatric department from January, 2014 to December 2014. Total 1009 newborns blood sample with suspected and clinical sepsis were cultured by using standard microbiological technique and antibiotic sensitivity patterns were studied. Results: The positive blood culture was 32.4% (327/1009. Gram positive bacteria were more common 231(71% than gram negative bacteria 96(29%. Staphylococcus aureus 174 (53.2% and acinetobacter 46(14.1% were the commonest isolates in blood culture. Most of the organisms showed sensitivity with aminoglycosides (gentamicin and amikacin and third generation cephalosporins.Conclusion: Staphylococcus aureus, Acinetobacter and Klebsiella species remain the principal organisms causing neonatal sepsis and antibiotics like amino glycosides should be first choice of drugs.

  15. Evaluation of bacteriology of middle ear in early quiescent stage of chronic otitis media

    Directory of Open Access Journals (Sweden)

    Ramesh Bhandari

    2013-09-01

    Full Text Available Objectives The objectives of this study were to determine whether any organism does exist in middle ear cavity during the early quiescent stage of chronic otitis media and to isolate their types. Materials and methods Forty-seven patients of age 13 years and above with diagnosis of chronic otitis media mucosal type in early quiescent stage were included. Swab was collected from middle ear cavity for culture and sensitivity in operation theatre prior to middle ear surgery and brought to microbiology laboratory within half an hour to inoculate in Blood agar, Chocolate agar and Mac Conkey agar. The isolates were identified with the use of standard bacteriological technique. Results Aerobic bacteria were isolated from 15 cases (31.9%. Staphylococcus aureus isolated in 12(80%, Pseudomonas aeruginosa in 2(13.3% and E. coli in 1(6.7%. Conclusion Aerobic bacteria were isolated from middle ear cavity in quiescent stage of chronic otitis media in 15(32% cases. Staphylococcus aureus was the most common organism. Journal of College of Medical Sciences-Nepal, 2012, Vol-8, No-4, 22-26 DOI: http://dx.doi.org/10.3126/jcmsn.v8i4.8696

  16. Bacteriological evaluation of fresh chicken sausage submitted to gamma radiation

    International Nuclear Information System (INIS)

    Borges, Alexandre

    2002-01-01

    light of the lack of present data on the irradiation of fresh chicken sausage, the objective of this study is to evaluate determine the efficiency of this method as a process for reducing the microbial load via measurement of the bacteriology of the product before and after gamma radiation. 45 sample of chicken sausage(acquired directly from the poultry industry) were prepared divided in 3 treatments: the first is a control, the second and third using application of 1,5 and 3,0 kGy, respectively. The chicken sausages irradiated with a dose of 1,5 kGy did not have, statistically significant results apparent in the bacteriological measures. Samples irradiated with 3,0 kGy did experience statistically significant reductions. It is concluded that the irradiating the product with a dose of 3,0 kGy is more effective for the reduction of bacterial population, with the data in these trials indicating a 95,3% reduction in total coliforms, 100% removal of fecal coliforms, and 84,0% reduction of mesophiles, relative to the control samples. (author)

  17. Bacteriology of drinking water distribution systems: an integral and multidimensional review.

    Science.gov (United States)

    Liu, G; Verberk, J Q J C; Van Dijk, J C

    2013-11-01

    A drinking water distribution system (DWDS) is the final and essential step to supply safe and high-quality drinking water to customers. Biological processes, such as biofilm formation and detachment, microbial growth in bulk water, and the formation of loose deposits, may occur. These processes will lead to deterioration of the water quality during distribution. In extreme conditions, pathogens and opportunistic pathogens may proliferate and pose a health risk to consumers. It is, therefore, necessary to understand the bacteriology of DWDSs to develop effective strategies that can ensure the water quality at consumers' taps. The bacteriology of DWDSs, both the quantitative growth and the qualitative bacterial community, has attracted considerable research attention. However, the researchers have focused mainly on the pipe wall biofilm. In this review, DWDS bacteriology has been reviewed multidimensionally, including both the bacterial quantification and identification. For the first time, the available literature was reviewed with an emphasis on the subdivision of DWDS into four phases: bulk water, suspended solids, loose deposits, and pipe wall biofilm. Special concentration has been given to potential contribution of particulate matter: suspended particles and loose deposits. Two highlighted questions were reviewed and discussed: (1) where does most of the growth occur? And (2) what is the contribution of particle-associated bacteria to DWDS bacteriology and ecology? At the end of this review, recommendations were given based on the conclusion of this review to better understand the integral DWDS bacteriology.

  18. [Bacteriological aspects of preparation, storage and transport of milk formulas (author's transl)].

    Science.gov (United States)

    Irle, U; Gängel, G

    1976-07-01

    1. It was shown that special apparatus for cleaning milkbottles makes additional desinfection unnecessary with regard to bacteriological and hygienic aspects. -- 2. Milk-bottles should be processed centrally for safety reasons. Special basket-containers facilitate a more economic washing process, transport and storage of the bottles. -- 3. Physiologically optimal milk formulas, as available today, should be prepared and filled into bottles in the ward where they are needed, immediately before use, to guarantee bacteriologically optimal conditions. -- 4. With appropriate organization within a clinic it should be possible to store special dietary formulas which have been prepared centrally, not in the wards, over the required time without the risk of bacterial contamination. The need for regular and systemic bacteriological checks is emphasized.

  19. [Current status of bacteriological studies at prefectural and municipal public health institutes in Japan].

    Science.gov (United States)

    Shinomiya, Hiroto; Seto, Kazuko; Kawase, Jun; Arikawa, Kentaro; Funatogawa, Keiji; Suzuki, Masahiro; Kubota, Hiroaki; Shirabe, Komei

    2015-01-01

    Prefectural and municipal public health institutes are located in prefectures and ordinance-designated cities in Japan, and play a vital role in the regional surveillance of infectious diseases and foodborne illnesses. These institutes, in close cooperation with national institutes such as the National Institute of Infectious Diseases and the National Institute of Health Sciences, construct the national surveillance network for infectious diseases and their causative agents. Bacteriological examinations and studies on a variety of infectious diseases and foodborne illnesses are core activities of prefectural and municipal public health institutes, through which novel and important bacteriological findings have been acquired. In this article, we report the latest findings regarding bacteriological examinations/studies and interesting cases at these institutes, especially concerning foodborne illnesses, tuberculosis, and antimicrobial resistances.

  20. BACTERIOLOGICAL ANALYSIS AND PUBLIC PERCEPTION ABOUT DRINKING WATER OF BOREHOLES IN ARIB (AIN DEFLA, ALGERIA

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    F. C. Hamaidi

    2016-05-01

    Full Text Available Water from boreholes in Arib (Ain Defla, south-west of Algeria was investigated to ascertain its quality status and suitability for drinking and domestic uses. Fifty six water samples were collected from four boreholes. The analyses of the Coliform counts obtained from various boreholes samples ranged between 0 and 200 CFU/ 100 ml with regard to total Coliforms and between 0 and 8 CFU/ 100 ml with regard to fecal Coliforms. Three of the boreholes samples showed contamination by Streptococcus sp. Pathogens like Salmonella were not identified but some low levels of sulfite-reducing bacteria was found. The interviews were done to assess perceptions on water taste problems, odour, colour, and turbidity and health problems. The respondents were taken from people in the community using random sampling technique. Sixty people were sampled at each area surrounding the boreholes. It was found out that 43.3% of the respondents rated their drinking water safe for consumption as well as absence of illness after drinking as indicators for judging the quality of the water. The others respondents (56.7% reported having at least some concerns with safety of their water.  More  than 20% of the households reported at least one household member having suffered some water related illness in the past two years. Respondents were asked to judge the quality of the water, based on four sensory characteristics of drinking water. Many respondents rated the smell (33.3%, taste (20%, colour (23.3% and turbidity (23.3%. These results showed that all the samples did not satisfy the WHO requirements for bacteriological characteristics in human consumption.

  1. BACTERIOLOGICAL PROFILE OF PATIENTS WITH ACUTE PYOGENIC MENINGITIS - A HOSPITAL BASED STUDY

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    Arnab

    2016-03-01

    Full Text Available BACKGROUND Pyogenic meningitis is one of the most common infectious disease emergencies involving the central nervous system with higher incidence in developing countries than developed nations. Despite the large number of pathogens that have been reported to cause acute pyogenic meningitis, certain microorganisms are isolated with higher frequency depending on patient’s age, immune status and geography. Present study was aimed to determine the trends in aetiology and spectrum of the bacteriological profile in adult patients with suspected pyogenic meningitis in North-East India. MATERIALS 50 CSF samples from as many patients of Acute Bacterial Meningitis over a period of one year were processed for cell counts, biochemical analysis, gram staining, culture, antigen detection by latex agglutination test and antibiotic susceptibility tests, as per standard techniques. OBSERVATION CSF cell counts showed neutrophilic predominance in all cases along with high protein and low sugar levels. 44% of the cases were culture positive and latex agglutination test was positive in 46.4% of the cases where culture was negative. S. pneumonia was the predominant pathogen identified in the present study in 12(24% cases, followed by Pseudomonas and E. coli in 5(10% cases each. Gram stain indicated the causative organisms in 68.2% of the culture positive cases. Among the culture negative patients gram stain indicated the causative organism in 3(10.7% cases and these three cases were positive by LAT also. CONCLUSION Simple, rapid, inexpensive tests like gram staining remain significant means for diagnosis of acute pyogenic meningitis in developing countries. LAT goes a long way in identifying the organisms where the cultures are negative. This study thus paves the way for larger studies in this region for better recognition of the predominant organisms and the empirical antibiotic regimens.

  2. Monitoring of transmission of Salmonella enterica serovars in pigs using bacteriological and serological detection methods

    NARCIS (Netherlands)

    Winsen, van R.L.; Nes, van A.; Keuzenkamp, D.; Urlings, H.A.P.; Lipman, L.J.A.; Biesterveld, S.; Snijders, J.M.A.; Verheijden, J.H.M.; Knapen, van F.

    2001-01-01

    The standard method to detect Salmonella positive pigs is bacteriological examination of the faeces, but in recent years the use of Salmonella-ELISA’s have become available to screen pigs for serological evidence of infection. This study was conducted to monitor the transmission of five different

  3. 9 CFR 147.13 - Procedure for bacteriological culturing of eggshells for colon bacilli organisms.

    Science.gov (United States)

    2010-01-01

    ... culturing of eggshells for colon bacilli organisms. 147.13 Section 147.13 Animals and Animal Products ANIMAL... bacteriological culturing of eggshells for colon bacilli organisms. Proper precautions to avoid environmental... conclusion of the presence of colon bacilli organisms. (Approved by the Office of Management and Budget under...

  4. Bacteriological Evaluation Of Pre-Cut Fruits Sold In Kano Metropolis ...

    African Journals Online (AJOL)

    One hundred and fifty (150) pre-cut fruit samples comprising of Pineapples (50), Paw-paw (50) and Watermelon (50) at the point of stand retail outlets were tested by standard bacteriological methods to determine bacterial contamination of the fruits. Out of these 150 examined 136 (90.67%) were contaminated with bacteria.

  5. Bacteriological qualities of indoor and out-door drinking water in ...

    African Journals Online (AJOL)

    Objectives: To compare the bacteriological quality of out-house (tank or standpipe) water and in-house drinking water (storage containers) and determine the risk factors influencing it. Design: A cross-sectional study. Setting: The study was carried out in Kibera slums located 7 km southwest from the Nairobi City centre.

  6. Evaluation of Bacteriological Stability of Minced Canned Meat ...

    African Journals Online (AJOL)

    Thermal inactivation of spoilage microorganisms is one of the widely used commercial food preservation techniques. However, its application may be too costly in terms of energy expenditure or inappropriate in terms of product quality. In this study, an attempt was made to produce tropical storage stable canned meat using ...

  7. aerobic bacteriological profile and antibiotic resistance of surgical ...

    African Journals Online (AJOL)

    2015-07-06

    Jul 6, 2015 ... Inspite of advances in surgical techniques, better operating room environment, antibiotic prophylaxis, Surgical Site Infections still occurs in significant number of patients. Surgical site infections (SSIs) infections occur within thirty days after the operative procedure (except in case of added implants, when the ...

  8. Bacteriological and elemental quality of Clarias gariepinus (cat fish ...

    African Journals Online (AJOL)

    Methodology: Three live cat fish (Clarias gariepinus) were obtained from River Lavun monthly for six months for analyses. Using standard methods, microbial load was determined, while susceptibility profiles of the identified isolates was obtained using disc diffusion technique. Elemental contents of the fish were determined ...

  9. PHYSICOCHEMICAL AND BACTERIOLOGICAL WATER QUALITY OF THE TEPALCINGO-AXOCHIAPAN AQUIFER, MORELOS, MEXICO

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    Esperanza S. Robles

    2013-03-01

    Full Text Available The water quality of the Tepalcingo-Axochiapan aquifer, Mexico was determined. Six samplings were carried out, taking samples from eight wells before chlorine and one spring. Two bacteriological and 11 physicochemical parameters were analyzed. Most of the wells and spring presented hard water. The wells showed higher concentrations of dissolved solids at lower altitudes, exception of the spring. Discriminate analysis and the Mahalanobis distances showed that the spring had the biggest differences with respect to the other wells. Bacteriologically the spring and a well are not suitable for amusement activities and physicochemically three wells are not suitable as a source of drinking water. The lack of sanitation and drainage in some areas may be causing deterioration of water quality in the aquifer in such areas.

  10. Bacteriological Monitoring and Sustainable Management of Beach Water Quality in Malaysia: Problems and Prospects

    Science.gov (United States)

    Dada, Ayokunle Christopher; Asmat, Ahmad; Gires, Usup; Heng, Lee Yook; Deborah, Bandele Oluwaseun

    2012-01-01

    Despite the growing demand of tourism in Malaysia, there are no resolute efforts to develop beaches as tourist destinations. With no incentives to monitor public beaches or to use them in a sustainable manner, they might eventually degenerate in quality as a result of influx of pollutants. This calls for concerted action plans with a view to promoting their sustainable use. The success of such plans is inevitably anchored on the availability of robust quality monitoring schemes. Although significant efforts have been channelled to collation and public disclosure of bacteriological quality data of rivers, beach water monitoring appears left out. This partly explains the dearth of published information related to beach water quality data. As part of an on-going nation-wide surveillance study on the bacteriological quality of recreational beaches, this paper draws on a situation analysis with a view to proffering recommendations that could be adapted for ensuring better beach water quality in Malaysia. PMID:22980239

  11. Bacteriological monitoring and sustainable management of beach water quality in Malaysia: problems and prospects.

    Science.gov (United States)

    Dada, Ayokunle Christopher; Asmat, Ahmad; Gires, Usup; Heng, Lee Yook; Deborah, Bandele Oluwaseun

    2012-04-28

    Despite the growing demand of tourism in Malaysia, there are no resolute efforts to develop beaches as tourist destinations. With no incentives to monitor public beaches or to use them in a sustainable manner, they might eventually degenerate in quality as a result of influx of pollutants. This calls for concerted action plans with a view to promoting their sustainable use. The success of such plans is inevitably anchored on the availability of robust quality monitoring schemes. Although significant efforts have been channelled to collation and public disclosure of bacteriological quality data of rivers, beach water monitoring appears left out. This partly explains the dearth of published information related to beach water quality data. As part of an on-going nation-wide surveillance study on the bacteriological quality of recreational beaches, this paper draws on a situation analysis with a view to proffering recommendations that could be adapted for ensuring better beach water quality in Malaysia.

  12. [Bacteriological profile of infectious endocarditis in the area of Sfax (Tunisia)].

    Science.gov (United States)

    Hammami, N; Mezghani, S; Znazen, A; Rhimi, F; Kassis, M; Hakim, H; Dammak, J; Kamoun, S; Hammami, A

    2006-01-01

    We performed a study to analyze epidemiological characteristics and bacteriological profile of infectious endocarditis (I.E) in the area of Sfax (Tunisia). We analyzed, retrospectively, all cases of I.E, according to Duke Criteria, hospitalized in the CHU Hédi Chaker of Sfax between January 1997 and December 2000. Bacteriological investigation included blood culture, cardiac valve culture and serology. Seventy-two cases of I.E were diagnosed. The average of age was 32.3 years. 47.3% of the patients did not have cardiac disease, 25% had a valvular prosthesis, 20.8% a native valvulopathy and 6.9% a congenital cardiopathy. Antecedent of acute rheumatic fever was noted in 66% of I.E on native valvulopathy and in 55.5% of I.E on prosthesis. The mitral valve was involved in 39%, the aortic in 27.5% and the two in 26% of the cases. The origin of bacteremia was found or supposed in 55.5% of the cases and was commonly dental (33 % of EI). The bacteriological diagnosis was positive in 51 cases (70.8%). Staphylococci were isolated in 17 cases (23.6%), Streptococci in 17 cases (23.6%) and dominated by oral streptococci (12 cases). Chlamydial serology was positive in 8 cases (11.1%). Diagnosis of infectious endocarditis due to Chlamydia pneumoniae was confirmed in a case by genomic amplification (PCR) and in situ hybridization on the valve. Endocarditis in Tunisia remains frequent. It reaches with predilection the young person in particular with rheumatic heart diseases. The bacteriological profile remains dominated by Streptococci and the Staphylococci.

  13. Hematology, blood chemistry, and bacteriology of the free-ranging Mexican beaded lizard (Heloderma horridum).

    Science.gov (United States)

    Espinosa-Avilés, David; Salomón-Soto, Víctor Manuel; Morales-Martínez, Socorro

    2008-03-01

    Blood samples from 16 adult, wild Mexican beaded lizards were obtained for complete blood count and serum biochemical analysis. In addition, both oral and cloacal swab samples were collected and submitted for bacteriologic culture. This is the first report of hematologic and bacterial data from free-living beaded lizards. This information will serve as baseline reference values for future health assessment studies in conservation, captive breeding, and research programs.

  14. PRELIMINARY INVESTIGATION ON THE BACTERIOLOGICAL QUALITY OF MICROFILTERED DRINKING WATER DISPENSERS IN CATERING ESTABLISHMENTS

    OpenAIRE

    M.A. Marzano; C.M. Balzaretti

    2011-01-01

    The bacteriological quality of microfiltered drinking water dispensers was evaluated, through enumeration of heterotrophic plate count at 22 and 37 °C, total coliforms, Escherichia coli, Enterococcus spp. and Pseudomonas aeruginosa. The aim of this research was to control the hygiene of the microfiltered water and to evaluate the effectiveness of the microfiltration procedure on the survival of bacteria. In total, 54 water samples were analyzed. The results indicated a high contamination ...

  15. [An approach to bacteriological taxonomy by application of Immanuel Kant's transcendental dialectics (author's transl)].

    Science.gov (United States)

    Habs, H

    1981-01-01

    After having altered the name of International Committee for Bacteriological Nomenclature in International Committee on Systematic Bacteriology in 1970, the latter will also have to reflect upon the objects of taxonomy. An approach thereto is recognizable in the revision of the International Code of Nomenclature of Bacteria in 1975. Considerations are being made whether a classification of bacteria does justice to the laws of homogenicity, specification and continuity as laid down by Kant in his transcendental dialectic. Most important of all are definition and determination of the taxon species. As far as contents go the latter is not possible from the biological point of view but applicable to its range in application of the regulations of the code. Within the priorities of taxa the species adopts a preferential position because conceptions of applied bacteriology are contained therein. The variety of infra-subspecific subdivisions is taken into consideration; as far as the formae speciales are concerned considerations as made with regard to species apply.

  16. Culture methods of allograft musculoskeletal tissue samples in Australian bacteriology laboratories.

    Science.gov (United States)

    Varettas, Kerry

    2013-12-01

    Samples of allograft musculoskeletal tissue are cultured by bacteriology laboratories to determine the presence of bacteria and fungi. In Australia, this testing is performed by 6 TGA-licensed clinical bacteriology laboratories with samples received from 10 tissue banks. Culture methods of swab and tissue samples employ a combination of solid agar and/or broth media to enhance micro-organism growth and maximise recovery. All six Australian laboratories receive Amies transport swabs and, except for one laboratory, a corresponding biopsy sample for testing. Three of the 6 laboratories culture at least one allograft sample directly onto solid agar. Only one laboratory did not use a broth culture for any sample received. An international literature review found that a similar combination of musculoskeletal tissue samples were cultured onto solid agar and/or broth media. Although variations of allograft musculoskeletal tissue samples, culture media and methods are used in Australian and international bacteriology laboratories, validation studies and method evaluations have challenged and supported their use in recovering fungi and aerobic and anaerobic bacteria.

  17. A proposal for further integration of the cyanobacteria under the Bacteriological Code.

    Science.gov (United States)

    Oren, Aharon

    2004-09-01

    This taxonomic note reviews the present status of the nomenclature of the cyanobacteria under the Bacteriological Code. No more than 13 names of cyanobacterial species have been proposed so far in the International Journal of Systematic and Evolutionary Microbiology (IJSEM)/International Journal of Systematic Bacteriology (IJSB), and of these only five are validly published. The cyanobacteria (Cyanophyta, blue-green algae) are also named under the Botanical Code, and the dual nomenclature system causes considerable confusion. This note calls for a more intense involvement of the International Committee on Systematics of Prokaryotes (ICSP), its Judicial Commission and its Subcommittee on the Taxonomy of Photosynthetic Prokaryotes in the nomenclature of the cyanobacteria under the Bacteriological Code. The establishment of minimal standards for the description of new species and genera should be encouraged in a way that will be acceptable to the botanical authorities as well. This should be followed by the publication of an 'Approved List of Names of Cyanobacteria' in IJSEM. The ultimate goal is to achieve a consensus nomenclature that is acceptable both to bacteriologists and to botanists, anticipating the future implementation of a universal 'Biocode' that would regulate the nomenclature of all organisms living on Earth.

  18. Broad-range PCR: past, present, or future of bacteriology?

    Science.gov (United States)

    Renvoisé, A; Brossier, F; Sougakoff, W; Jarlier, V; Aubry, A

    2013-08-01

    PCR targeting the gene encoding 16S ribosomal RNA (commonly named broad-range PCR or 16S PCR) has been used for 20 years as a polyvalent tool to study prokaryotes. Broad-range PCR was first used as a taxonomic tool, then in clinical microbiology. We will describe the use of broad-range PCR in clinical microbiology. The first application was identification of bacterial strains obtained by culture but whose phenotypic or proteomic identification remained difficult or impossible. This changed bacterial taxonomy and allowed discovering many new species. The second application of broad-range PCR in clinical microbiology is the detection of bacterial DNA from clinical samples; we will review the clinical settings in which the technique proved useful (such as endocarditis) and those in which it did not (such as characterization of bacteria in ascites, in cirrhotic patients). This technique allowed identifying the etiological agents for several diseases, such as Whipple disease. This review is a synthesis of data concerning the applications, assets, and drawbacks of broad-range PCR in clinical microbiology. Copyright © 2013 Elsevier Masson SAS. All rights reserved.

  19. Bench-top validation testing of selected immunological and molecular Renibacterium salmoninarum diagnostic assays by comparison with quantitative bacteriological culture

    Science.gov (United States)

    Elliott, D.G.; Applegate, L.J.; Murray, A.L.; Purcell, M.K.; McKibben, C.L.

    2013-01-01

    No gold standard assay exhibiting error-free classification of results has been identified for detection of Renibacterium salmoninarum, the causative agent of salmonid bacterial kidney disease. Validation of diagnostic assays for R. salmoninarum has been hindered by its unique characteristics and biology, and difficulties in locating suitable populations of reference test animals. Infection status of fish in test populations is often unknown, and it is commonly assumed that the assay yielding the most positive results has the highest diagnostic accuracy, without consideration of misclassification of results. In this research, quantification of R. salmoninarum in samples by bacteriological culture provided a standardized measure of viable bacteria to evaluate analytical performance characteristics (sensitivity, specificity and repeatability) of non-culture assays in three matrices (phosphate-buffered saline, ovarian fluid and kidney tissue). Non-culture assays included polyclonal enzyme-linked immunosorbent assay (ELISA), direct smear fluorescent antibody technique (FAT), membrane-filtration FAT, nested polymerase chain reaction (nested PCR) and three real-time quantitative PCR assays. Injection challenge of specific pathogen-free Chinook salmon, Oncorhynchus tshawytscha (Walbaum), with R. salmoninarum was used to estimate diagnostic sensitivity and specificity. Results did not identify a single assay demonstrating the highest analytical and diagnostic performance characteristics, but revealed strengths and weaknesses of each test.

  20. Bacteriological (fecal and total coliform) quality of Pakistani coastal water

    International Nuclear Information System (INIS)

    Mashiatullah, A.; Qureshi, R.M.; Javed, T.; Khan, M.S.; Chaudhary, M.Z.; Khalid, F.

    2010-01-01

    The coliform bacteria group consists of several genera of bacteria belonging to the family enterobacteriaceae. These are harmless bacteria, mostly live in soil, water, and digestive system of animals. Fecal coliform bacteria, which belongs to this group, are present in large numbers in feces and intestinal tract of human beings and other warm-blooded animals which can enter into water bodies from human and animal waste. Swimming in water having high levels of Fecal coliform bacteria increases the chance of developing illness (fever, nausea or stomach cramps) from pathogens entering the body through mouth, nose, ears or cuts in the skin. The objective of the present study was to characterize the bathing quality of Pakistani coastal water with respect to coliform bacteria. Total and Fecal coliform bacteria were determined at seven different locations along Pakistan coast using membrane filtration (MF) technique. 100 ml of water was passed through 0.45 micron (mu) filter paper. These filter papers were put on pads, soaked in Lauryle sulphate broth in petri-dishes and incubated at 44 deg. C for Fecal and 37 deg. for Total coliform for 24 hours. Significantly high population of Fecal and Total coliform bacteria was recorded at Karachi harbour area and Indus delta region. Results indicate that a large amount of domestically originated waste is being discharged into these locations without any pre-treatment (e.g., screening, activated sludge, by using filtration beds etc.) resulting in a poor seawater quality making it unfit for bathing. (author)

  1. Bacteriological study of urinary tract infection in antenatal care patients

    Directory of Open Access Journals (Sweden)

    Srivastava Ritu, Singh Brij N, Begum Rehana, Yadav Ramesh

    2014-04-01

    Full Text Available Aims & Objective: To isolate and diagnose the Uropathogens and its antibiotic sensitivity pattern in anti-natal care patient suffering from Urinary tract Infections. Material and Methods: 150 samples were collected by consent pregnant women between the age group of 18 to 40 years. A midstream clean catch is adequate, provided by all pregnant women’s through given careful instructions. For enumeration of bacteria we perform standard loop techniques method. The number of colonies counted or estimated, and this number used to calculate the number of viable bacteria per ml of urine. The bacterial strains were identified by colonies character stick, gram staining, morphological and biochemical character. The bacterial strains identification was done up to genus and species level. The antibiotics sensitivity test of bacterial strains was done as per CLSI guidelines by Kirby-Baure Disc Diffusion Methods. Results: The significant bactiurea was found in 50 patients among 150 patients used. The most commonly isolated bacteria was Escherichia coli 23(40% Klebsiellaaerogens 11 (22% Staphylococcus aureus 10 (20% Pseudomonas aerugenosa 4(8%.The incidence of bacteriuria among in their first pregnancy was 22.2%.The higher incidence of UTI in 2nd and 3rd trimester was found to have 31.4% & 40%. These studies were showing high level of resistance to first line antibiotics such as Cotrimaxozole. Conclusion: To minimizing the complication of the pregnant women should be educated about the physiology of pregnancy clinical presentation includes asymptomatic bacteria, acute cystitis & pyelonephritis. Pregnant women should be screened for asymptomatic bacteriuria by urine culture and treated with appropriate antibiotics. After the post treatment pregnant women should be examine again to confirm post treatment urine sterility.

  2. PRELIMINARY INVESTIGATION ON THE BACTERIOLOGICAL QUALITY OF MICROFILTERED DRINKING WATER DISPENSERS IN CATERING ESTABLISHMENTS

    Directory of Open Access Journals (Sweden)

    M.A. Marzano

    2011-04-01

    Full Text Available The bacteriological quality of microfiltered drinking water dispensers was evaluated, through enumeration of heterotrophic plate count at 22 and 37 °C, total coliforms, Escherichia coli, Enterococcus spp. and Pseudomonas aeruginosa. The aim of this research was to control the hygiene of the microfiltered water and to evaluate the effectiveness of the microfiltration procedure on the survival of bacteria. In total, 54 water samples were analyzed. The results indicated a high contamination frequency with P. aeruginosa (25 and 20% in room temperature and chilled water samples, respectively and therefore the need to improve the efficacy and the frequency of the dispenser sanitation procedures.

  3. A Clinico-bacteriological study of primary pyodermas of children in Pondicherry

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    Mathew Mariette

    1992-01-01

    Full Text Available One hundred and twenty children selected at random and diagnosed as having Primary Pyoderma lesions were studied for clinical patterns and bacteriological profile. Sixty percent of the cases were girls. The commonest clinical type was impetigo contagiosa (45% followed by folliculities of the scalp (44.2%. Staphylococcus aureus was the etiological agent in 47.5% while 26.7% of the cases were due to mixed infection along with Streptococcus pyogenes. Almost all strains were sensitive to Erythromycin and Gentamycin. The highest resistance was to Penicillin (79.3% followed by Ampicillin (73.9% and Tetracycline (42.3%.

  4. Bacteriological status of beef carcasses at a commercial abattoir before and after slaughterline improvements.

    Science.gov (United States)

    Hudson, W. R.; Roberts, T. A.; Whelehan, O. P.

    1987-01-01

    The bacteriological status of beef carcasses was monitored at a commercial abattoir before and after two stages of modernization to the beef slaughterline which included changing from cradle dressing to dressing on an overhead rail, and the introduction of hot water spray cleaning of carcasses. Although small significant (P less than 0.05) differences in bacterial count occurred among carcass sites within modernization stages, significant visit within stage variation and stage X site interactions prevented any significant change in overall count being observed among stages and carcass sites. Principal components analysis revealed small changes in the distribution of bacterial numbers on the sites sampled. PMID:3556439

  5. Clinical, Bacteriologic, and Geographic Stratification of Melioidosis Emerges from the Sri Lankan National Surveillance Program.

    Science.gov (United States)

    Sathkumara, Harindra D; Merritt, Adam J; Corea, Enoka M; Krishnananthasivam, Shivankari; Natesan, Mohan; Inglis, Timothy J J; De Silva, Aruna Dharshan

    2018-02-01

    Melioidosis, a potentially fatal tropical infection, is said to be underdiagnosed in low-income countries. An increase in melioidosis cases in Sri Lanka allowed us to analyze the relationship among clinical outcome, bacteriology, epidemiology, and geography in the first 108 laboratory-confirmed cases of melioidosis from a nationwide surveillance program. The additional 76 cases of laboratory-confirmed melioidosis confirmed further associations between Burkholderia pseudomallei multilocus sequence typing (MLST) and infection phenotype; ST1137/unifocal bacteremic infection (χ 2 = 3.86, P national genotyping-supported melioidosis registry will improve melioidosis diagnosis, treatment, and prevention where underdiagnosis and mortality rates remain high.

  6. Problems of Bacteriological Pollution in Water Wells in Wadi Hadramout Water Project

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    Salem M. Bin Qadhi

    2001-12-01

    Full Text Available This paper presents an existing problem of drinking water pollution for a small project and one of the model projects of drinking water in Yemen, designed to save and preserve good quality water for the present and future generations in the area. The paper gives the details of the project and explains the problem of bacteriological pollution and the steps undertaken to solve the problem. Some of the results for short-term solutions and recommendation for the long-term solutions are also given.

  7. Aseptic techniques(practical guide no.3)

    International Nuclear Information System (INIS)

    Nappa, Andres; Souto Pais, Beatriz

    1994-01-01

    Definition of aseptic techniques in relation with the labor of Radiopharmacy.Control of environment and working areas.Control of materials entered into an aseptic area: water, nitrogen,vials,stoppers,needles and syringes. Personnel and manipulations control,choosing of materials,precautions in the aseptic manipulation and sterility preservation during usage life. Bacteriological membrane filtration in sterilized conditions. Work in a laminar flow unit. Protocol of Practical task 3. Bibliography

  8. Bacteriological quality

    African Journals Online (AJOL)

    User

    ), Enterobacter cloacae(1), Serratia liqueofaciens(1), Enterobacter sakazaki(1), Serratia marcesense(1), Klebsella orithinolytica(4), and Escherichia coli(1) were identified to species level. All isolates were sensitive to Amikacin (AK), Norfloxacin.

  9. Bacteriological evaluation of refrigerated vacuum and air-packed chicken fillets treated with irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Mantilla, Samira P.S.; Santos, Erica B.; Mano, Sergio B.; Franco, Robson M. [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil). Dept. de Tecnologia de Alimentos], e-mail: samiramantilla@yahoo.com.br, e-mail: ericaebs@hotmail.com, e-mail: mtasbm@vm.uff.br, e-mail: robsonmf@vm.uff.br; Conte Junior, Carlos A. [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil)], e-mail: carlosconte@hotmail.com; Vital, Helio C. [Centro Tecnologico do Exercito (CTEx), Rio de Janeiro, RJ (Brazil). Div. de Defesa Quimica, Biologica e Nuclear (DDQBN)], e-mail: vital@ctex.eb.br

    2009-07-01

    Chicken meat is a nutritious food, rich in essential aminoacids and much appreciated by a large fraction of the population. However, it is also highly perishable, typically having a shelf life of 5 to 7 days in refrigeration, depending on the initial microbiological load. Irradiation has been efficiently used to improve safety and extend the shelf lives of many meat products. Its use in combination with refrigeration and exclusion of oxygen is known to greatly enhance the sanitary quality of meat. This work investigated the bacteriological effects of radiation doses of 0; 2.0 and 3.0 kGy on vacuum- and air-packed chicken fillets kept at 1 deg C for up to 18 days. Bacteriological analyses that included enumerating and counting indicated that both the lag phase of the bacterial growth and the shelf life of the samples increased with dose. It was observed that exposure to 3.0 kGy extended the initial 5-day shelf life of the air-packed fillets to 10 days while prolonging to 12 days the shelf life of the vacuum-packed ones. Among the species of bacteria monitored, the lactic bacteria were found to be the most resistant to gamma radiation while coliforms were the most sensitive. (author)

  10. Clinical, bacteriologic, serologic, and pathologic features of infections with atypical Taylorella equigenitalis in mares.

    Science.gov (United States)

    Katz, J B; Evans, L E; Hutto, D L; Schroeder-Tucker, L C; Carew, A M; Donahue, J M; Hirsh, D C

    2000-06-15

    To characterize clinical, serologic, bacteriologic, cytologic, and pathologic endometrial responses of mares to 2 donkey-origin atypical bacterial isolates resembling Taylorella equigenitalis. Prospective in vivo study. 10 healthy mares. Mares in estrus (2/group) were inoculated by intrauterine infusion with 2 isolates of classic T equigenitalis or 2 isolates of atypical Taylorella sp or were sham-inoculated. Bacteriologic, serologic, clinical, uterine, cytologic, and pathologic endometrial responses were assessed 4, 11, 21, 35, and 63 days after inoculation and on day 111 in mares with positive culture results on day 63. One atypical isolate failed to cause infection. The second atypical isolate and both classic T equigenitalis isolates induced similar transient metritis and cervicitis. Both classic isolates and 1 atypical isolate induced anti-T equigenitalis complement-fixing antibodies detectable at day 11. Classic isolates and an atypical isolate provoked intense neutrophilic endometritis followed by a resolving, subacute, neutrophilic-mononuclear endometrial response. The atypical isolate and classic isolates were recovered from the uterus, clitoral fossa, or clitoral sinus of one or both exposed mares for as long as 111 days. Atypical Taylorella sp infections should be considered as a differential diagnosis of equine infertility in US-origin mares, even those not exposed to stallions from countries where contagious equine metritis occurs. The origins and prevalence of atypical Taylorella sp infection in US horses and donkeys are undetermined.

  11. Bacteriological Study of the Marine Water in the Coastal of the North Sulawesi Province, Indonesia

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    Lies Indah Sutiknowati

    2006-11-01

    Full Text Available The main objective of this research was to study the marine bacteriology of the coast of North Sulawesi. The study was accomplished by calculating the abundance of coliform, heterotrophic, and pathogenic bacteria, and analyzing the coexistence relationship between bacteria and phytoplanktons. This research, which included the sampling and laboratory works, has been carried out on 25 - 28 October, 2000. The results suggested that the abundance of each bacteria was as follows: coliform bacteria range between 227-5940 cfu/100 ml with averages 1814.1 cfu/100 ml, found in all stations; heterotrophic bacteria range between (1-82 x 103 cfu/ml with averages 12.1 x 103 cfu/ml, it was high density and has association with phytoplankton Trichodesmium thieubautii. It was also found 6 species of pathogen bacteria e.g. Aeromonas, Citrobacter, Proteus, Pseudomonas, Yersinia and Shigella. The presence of coliform and pathogen bacteria was indicator of low quality of the seawater in the sampling area. Based on bacteriological study, the North Sulawesi Coastal is not suitable for aquaculture and need treatment and controlled for further coastal exploitation.

  12. Histopathological and Bacteriological Analysis of Thrombus Material Extracted During Mechanical Thrombectomy in Acute Stroke Patients.

    Science.gov (United States)

    Hernández-Fernández, Francisco; Rojas-Bartolomé, Laura; García-García, Jorge; Ayo-Martín, Óscar; Molina-Nuevo, Juan David; Barbella-Aponte, Rosa Angélica; Serrano-Heras, Gemma; Juliá-Molla, Enrique; Pedrosa-Jiménez, María José; Segura, Tomás

    2017-12-01

    Management of stroke secondary to septic emboli (SE) remains challenging, due to both the lack of specific recommendations and the gravity of the underlying pathology.The aim of this study is to describe the presence of SE in a series of mechanical thrombectomies (MT), analyzing technical complexity and outcomes with respect to the patients by means of histological analysis and microbiological study of the clot. All the retrieved clots were studied under an established protocol, including histopathological and bacteriological study with hematoxylin-eosin, Gram and Gomori trichrome staining.Technical complexity in SE with respect to the series was evaluated by analyzing time of the procedures, number of passes and use of intracranial definitive stents. Over a 24-month period, bacteria were detected in the retrieved clot of four out of 65 patients (incidence 6.2%). Two cases were eventually diagnosed with infective endocarditis, while the remaining two were diagnosed with urinary tract infection and respiratory septicemia, respectively. Three of the four patients (75%) required an intracranial definitive stent in order to achieve successful recanalization.These procedures were significantly longer (137.7 vs. 59.8 min, p vs. 2.2, p vs. 1.6%, p = 0.008), with respect to the rest of the series. In our series, systematic histopathological and bacteriological study of the MT samples allowed a higher proportion of SE diagnosis in comparison with previous reports.

  13. Bacteriological profile of neonatal septicemia in a tertiary care hospital from Western India

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    Vrishali Avinash Muley

    2015-01-01

    Full Text Available Neonatal septicemia is an important cause of morbidity and mortality. The present study was undertaken to determine the bacteriological profile and antimicrobial susceptibility pattern of prevalent pathogens isolated from the blood of septicemic neonates from Neonatal Intensive Care Unit (NICU. A total of 180 blood samples of septicemic neonates were studied bacteriologically. Antimicrobial susceptibility testing was done by the Kirby Bauer disc diffusion method in accordance to Clinical Laboratory Standards Institutes (CLSI guidelines. 26.6% (48 out of 180 cases of septicemia could be confirmed by blood culture. Of these, 66.7% cases were of early onset septicemia (EOS and 33.3% were of late onset septicemia (LOS. Klebsiella pneumoniae was the predominant pathogen (35.4% among the Gram-negative pathogens and Staphylococcus aureus (22.9% was the predominant Gram-positive pathogen. 28% of K. pneumoniae and E. coli isolates were extended spectrum beta-lactamase (ESBL producers. 18.1% of the Staphylococcus isolates were methicillin-resistant S. aureus (MRSA. Multi-drug-resistance pattern was observed with all the isolates. Ciprofloxacin and aminoglycosides were the most effective drugs against Gram-positive and Gram-negative isolates. This study highlights the predominance of Gram-negative organisms in causing neonatal sepsis and emergence of multi-drug-resistant strains in our set up.

  14. Evaluation of the Bacteriological Quality of Dental Unit’s Waterlines, Tehran, Iran

    Directory of Open Access Journals (Sweden)

    Hossein Masoumbeigi

    2017-10-01

    Full Text Available Oral and dentistry (O&D services processes may lead to exposing of personnel and patients with several microorganisms and arising of health problems. This cross-sectional study was investigated the bacteriological quality of dental unit waterlines (DUWLs in one of the O&D center in Tehran, Iran. One hundred ninety two samples were collected and examined based on standard microbiological procedures for determining and enumeration of heterotrophic plate count (HPC, Staphylococcus aureus and Pseudomonas aeruginosa. Data were analyzed by t-test, analysis of variance (ANOVA, Kruskal-Wallis and LSD tests with SPSS software (Ver.16. The results revealed that 70% of water samples (126 samples had a high density of contaminations that higher than recommended values for DUWLs quality. The mean of bacterial density on Saturday was more than Wednesday and was 1838 CFU/ml, 739 CFU/ml and 11 CFU/ml for HPC, P. aeruginosa, and S.aureuse respectively. The LSD test implied that the mean of bacterial density of inlet and outlet waters had significant statistical difference in various wards of the O&D center (p < 0.05. In addition, the results demonstrated that bacteriological quality in discharging water of various wards was higher than the recommended values. This research revealed that microbial water quality assessment in O&D services centers should be considered for providing of an appropriate disinfection procedure from point of infection control in dental operation services.

  15. Bacteriological evaluation of refrigerated vacuum and air-packed chicken fillets treated with irradiation

    International Nuclear Information System (INIS)

    Mantilla, Samira P.S.; Santos, Erica B.; Mano, Sergio B.; Franco, Robson M.; Vital, Helio C.

    2009-01-01

    Chicken meat is a nutritious food, rich in essential aminoacids and much appreciated by a large fraction of the population. However, it is also highly perishable, typically having a shelf life of 5 to 7 days in refrigeration, depending on the initial microbiological load. Irradiation has been efficiently used to improve safety and extend the shelf lives of many meat products. Its use in combination with refrigeration and exclusion of oxygen is known to greatly enhance the sanitary quality of meat. This work investigated the bacteriological effects of radiation doses of 0; 2.0 and 3.0 kGy on vacuum- and air-packed chicken fillets kept at 1 deg C for up to 18 days. Bacteriological analyses that included enumerating and counting indicated that both the lag phase of the bacterial growth and the shelf life of the samples increased with dose. It was observed that exposure to 3.0 kGy extended the initial 5-day shelf life of the air-packed fillets to 10 days while prolonging to 12 days the shelf life of the vacuum-packed ones. Among the species of bacteria monitored, the lactic bacteria were found to be the most resistant to gamma radiation while coliforms were the most sensitive. (author)

  16. Fabrication of autoclavable bacteriologic loops for handling Mycobacterium tuberculosis isolates from recycled materials in a resource poor setting.

    Science.gov (United States)

    Ochang, Ernest Afu

    2013-01-01

    In resource limited settings, the appropriation of scarce resources during research efforts can be daunting. Sourcing for disposable plastic bacteriological loops for manipulating M. tuberculosis had been eating into the research budget. In an attempt to reduce cost, an alternative and more cost effective way of obtaining autoclavable bacteriologic inoculation loops from used materials in the laboratory was employed. Autoclave resistant loops were prepared from polypropylene automatic pipette tips and platinum wires from electric stoves. The loop volume, when desired, was calculated using a simple mathematical equation after several passes in weighted water. Laboratories in resource poor settings could also save on inoculating loops by adopting such pragmatic approaches using recycled materials.

  17. Breast Abscessed Cancer in Nonlactating Women in Tropical Environment: Radiological, Bacteriological, and Anatomopathological Features about 3 Cases

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    Mazamaesso Tchaou

    2017-01-01

    Full Text Available The association of breast cancer and abscess is rare in daily practice. The authors report a short series of 3 cases of cancer of the breast in nonlactating women presented as breast abscess, reviewing aspects in radiology (ultrasound and mammography, correlating them with the histopathology findings and the bacteriological profile of the isolated germs.

  18. Comparative Bacteriological Study of Two Wild Boar Populations in Sierra Morena (Ja�n, Spain

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    Antonio NOTARIO

    2010-12-01

    Full Text Available The aim of this study was to investigate the presence of various bacterial species in the wild boar populations of Lugar Nuevo and Selladores-Contadero woodlands from Sierra Morena (Spain. Bacteriological analyses were carried out on a total of 229 wild boar individuals hunted in the period 2000-2003 in eleven experimental plots which are representative for the different biotopes of the area. The following species were detected: Brucella ovis, Clostridium sp., Corynebacterium sp., Streptococcus pneumoniae, Escherichia coli, Mycobacterium bovis, Mycobacterium avium subsp. paratuberculosis, Mannheimia haemolytica, Pasteurella multocida, Pseudomonas aeruginosa, Chlamydophila psittaci, Staphylococcus aureus and Staphylococcus sp. and the bacterial prevalence was estimated for each of them. The results provide useful indications of the health status of wild boar in both locations and highlight the potential of the wild boar populations to act as biological reservoirs of certain microorganisms that can be passed onto other vertebrate wild animals and humans.

  19. Bacteriological findings in patients with bone marrow transplantation (Karl Marx University Leipzig, 1985-1987).

    Science.gov (United States)

    Wonitzki, C; Hoffmann, F A

    1989-01-01

    The results of the bacteriological surveillance cultures for 26 patients with bone marrow transplantation (Karl Marx University Leipzig, G.D.R., 1985-1987) are presented. 5.9% of all surveillance cultures contained facultatively pathogenic germs (with Pseudomonas aeruginosa as the most frequent representative, which was the reason of a sepsis in two patients). Coagulasenegative Staphylococci and other germs with an obscure pathogenicity were isolated upon a large scale, especially from the mucous membrane regions. There are hints, that above all special strains of coagulasenegative Staphylococci "colonize" the patient's body (also for longer periods) and turn into the blood too. During the total decontamination intestinal anaerobic flora is absent. After closing of total decontamination Clostridium perfringens is the first detectable anaerobic species. During the selective decontamination systemic applications of antibiotics are able to obliterate anaerobic findings for certain periods. Recommendations for an effective arrangement of the surveillance cultures of bone marrow transplantation patients are given.

  20. Bacteriological quality of freshly processed broiler chickens as affected by carcass pretreatment and gamma irradiation

    International Nuclear Information System (INIS)

    Lamuka, P.O.; Sunki, G.R.; Chawan, C.B.; Rao, D.R.; Shackelford, L.A.

    1992-01-01

    Chicken carcasses dipped in whey fermented by Streptococcus thermophilus, lactic acid solution or water and irradiated at 2.5 kGy by 60Co were evaluated for bacteriological quality on day 1, day-3 and at 3-day intervals for an 18-day storage (4 degrees C) period. Unirradiated carcasses treated similarly were used as control. Gram negative bacteria, Yersinia and Campylobacter counts were significantly (p0.01) lower in irradiated samples, but no significant (p0.05) differences were observed ammong the dipping solutions. Salmonellae were completely eliminated in irradiated samples. Whey fermented by S. thermophilus reduced the proportion of Salmonella contaminated carcasses from 67% to 20%. As evidenced by the bacterial counts the shelf-life was found to be 15 days for irradiated carcasses compared to about 6 days for the unirradiated samples

  1. Bacteriological quality and solid wastes at five coastal marine environments of Costa Rica

    International Nuclear Information System (INIS)

    Garcia, Vera; Acuna Gonzalez, Jenaro; Vargas Zamora, Jose A.; Garcia Cespedes, Jairo

    2006-01-01

    Anthropogenic waste and water bacteriological quality were surveyed twice a year in 2000 and in 2002 at five coastal marine environments in Costa Rica, one in the Caribbean (Bahia de Moin) and four in the Pacific (Bahia de Culebra, Golfo de Nicoya, Estero de Puntarenas, Bahia de Golfito). The most probable number (MPN)/100 mL of coliform bacteria was calculated after incubation series of five test tubes. A total of 14 coastal and two river water samples were collected in the Caribbean, and 32 coastal, nine estuarine and one tap water samples in the Pacific, plus 25 samples investigated for Escherichia coli in 2002. The means of 2 MPN/100 mL in June 2000 and 17 MPN/100 mL in May 2002, and faecal coliforms [es

  2. Pharmacokinetics and bacteriological efficacy of mezlocillin in experimental Escherichia coli and Listeria monocytogenes meningitis.

    Science.gov (United States)

    Odio, C; Thomas, M L; McCracken, G H

    1984-04-01

    The purpose of this study was to determine the pharmacokinetics and bacteriological effect of mezlocillin in experimental meningitis caused by Listeria monocytogenes and two Escherichia coli strains. The half-life of mezlocillin in cerebrospinal fluid (CSF) was approximately twice that in serum of experimentally infected animals, and the penetration of drug into CSF was 5 to 15% after a single dose and 5 to 20% after continuous-infusion experiments. The bactericidal titer in CSF for both susceptible E. coli and L. monocytogenes was 1:8, whereas for the resistant E. coli strain, titers were less than 1:2 after single doses of 50 or 100 mg of mezlocillin per kg and 1:4 with continuous infusion. After single-dose and continuous-infusion experiments, the bacteriological effect of mezlocillin in experimental L. monocytogenes infections was similar to that of ampicillin. Mezlocillin reduced the colony counts of of susceptible E. coli in CSF by 90% or more after a single dose or continuous infusion but had no appreciable effect on resistant E. coli after a single dose of 50 mg/kg. In contrast, a single dose of 100 mg of mezlocillin per kg eradicated the resistant strain from CSF, despite a bactericidal titer in CSF of less than 1:2. This unexpected finding prompted us to evaluate the effect of serum on the in vitro susceptibilities of selected coliforms to mezlocillin. The activity of mezlocillin against one susceptible and four resistant strains of gram-negative, enteric bacilli was enhanced manyfold by the addition of fresh rabbit serum; this effect was abolished by heating the serum at 56 degrees C for 30 min. This interaction of mezlocillin and serum against coliform bacteria should be examined in a larger number of experimentally infected animals and in specimens obtained from mezlocillin-treated infants.

  3. The bacteriological composition of biomass recovered by flushing an operational drinking water distribution system.

    Science.gov (United States)

    Douterelo, I; Husband, S; Boxall, J B

    2014-05-01

    This study investigates the influence of pipe characteristics on the bacteriological composition of material mobilised from a drinking water distribution system (DWDS) and the impact of biofilm removal on water quality. Hydrants in a single UK Distribution Management Area (DMA) with both polyethylene and cast iron pipe sections were subjected to incremental increases in flow to mobilise material from the pipe walls. Turbidity was monitored during these operations and water samples were collected for physico-chemical and bacteriological analysis. DNA was extracted from the material mobilised into the bulk water before and during flushing. Bacterial tag-encoded 454 pyrosequencing was then used to characterize the bacterial communities present in this material. Turbidity values were high in the samples from cast iron pipes. Iron, aluminium, manganese and phosphate concentrations were found to correlate to observed turbidity. The bacterial community composition of the material mobilised from the pipes was significantly different between plastic and cast iron pipe sections (p < 0.5). High relative abundances of Alphaproteobacteria (23.3%), Clostridia (10.3%) and Actinobacteria (10.3%) were detected in the material removed from plastic pipes. Sequences related to Alphaproteobacteria (22.8%), Bacilli (16.6%), and Gammaproteobacteria (1.4%) were predominant in the samples obtained from cast iron pipes. The highest species richness and diversity were found in the samples from material mobilised from plastic pipes. Spirochaeta spp., Methylobacterium spp. Clostridium spp. and Desulfobacterium spp., were the most represented genera in the material obtained prior to and during the flushing of the plastic pipes. In cast iron pipes a high relative abundance of bacteria able to utilise different iron and manganese compounds were found such as Lysinibacillus spp., Geobacillus spp. and Magnetobacterium spp. Copyright © 2014 Elsevier Ltd. All rights reserved.

  4. Entero-hepatic pathobiology: histopathology and semi-quantitative bacteriology of the duodenum

    Directory of Open Access Journals (Sweden)

    NMK Ito

    2004-03-01

    Full Text Available Duodenum swabs were harvested from 80 broilers in 15 flocks of 8 broiler farms and were submitted to bacteriological evaluation. Five broiler breeders were sampled to obtain comparative data. Macro and microscopic lesions in the gut and the liver were evaluated in all birds submitted to swab harvesting and also broilers of two slaughter plants. Semiquantitative bacterial examination in duodenum swabs demonstrated higher microorganism growth in blood agar than in MacConkey medium. Staphylococcus sp, Enterococcus sp and Escherichia coli were found in some broiler flocks. An increased cellularity of the muscular mucosae of duodenum villi and/or the presence of some gametocytes or schizonts of coccidia were found in the lesioned gut in addition to high bacterial growth in blood agar. In some flocks, enlarged yellowish livers with vacuolated hepatocytes and/or increased reticuloendothelial response were seen in some birds. Liver and gut samples were collected in two slaughter plants in order to establish a pattern of response in broilers after withdrawal of growth promoters. Livers with macroscopic lesions presented microscopic lesions in the parenchyma such as increased leukocyte response in the sinusoidal space, vacuolar degeneration of hepatocytes or focal necrosis in the perilobular region, increased size or number of lymphoid nests in the perilobular region, the perivascular space and surrounding the biliary duct and increased extramedular granulocytopoiesis. Duodenum with hyperemic patches showed increased cellular response in the muscular mucosa of villi. These observations support the concept that semi-quantitative bacteriology and liver lesion evaluation could be used as a model to study gut bacterial imbalance in chickens.

  5. Potential sources of bacteriological pollution for two bays with marinas in Trinidad

    Directory of Open Access Journals (Sweden)

    Christine Ann Bullock

    2005-05-01

    Full Text Available Welcome Bay and Chaguaramas Bay in the northwest peninsula of Trinidad contain large marinas and smaller sections of bathing beaches.Bacteriological surveys were conducted at both bays to assess water quality and to determine potential sources of pollution.These surveys were conducted during the wet season of 1996 and the dry season of 1997.Eleven sample stations were established at Welcome Bay and 12 at Chaguaramas Bay.Freshwater samples were collected from rivers and drains within the survey area.Marine water samples were collected from marinas,bathing beaches and inshore and outer areas at both bays.Five water samples were collected from each sampling station during the wet season of 1996 and six during the dry season of 1997.The membrane filter technique was used to determine faecal coliform and Escherichia coli levels in all samples. There was a seasonal effect on water quality,with significantly higher faecal coliform levels in the wet season, when water quality was not in compliance with international standards.This represents a potential health risk in bathing areas.Water quality was better at the outer area of both bays.Water quality at the inner bay areas was most likely adversely affected by land-based sources of pollution identified in this study.These sources include three drains and two rivers,which discharged into the bays.Yachts were apparently not a source of sewage pollution:there was no significant relationship between yacht number and faecal coliform levels.Las bahías Welcome y Chaguaramas en la península noroeste de Trinidad tienen grandes marinas y secciones pequeñas de playas para bañistas.Se realizaron sondeos bacteriológicos en ambas bahías para determinar la calidad del agua y para señalar fuentes potenciales de contaminación.Estos sondeos fueron realizados durante la época lluviosa de 1996 y la seca de 1997.Once estaciones de muestreo se establecieron en Bahía Welcome y doce en Bahía Chaguaramas.Muestras de

  6. Geografía médica, bacteriología y el caso de las fiebres en Colombia en el siglo XIX

    Directory of Open Access Journals (Sweden)

    Mónica García

    2012-04-01

    Full Text Available This article explores the relation between bacteriology and medical geography in the 19th century in Colombia, following the case of fevers. Through this, it explains how historiography has approached the relation between bacteriology and medical geography, and analyzes said relation in the medical research pertaining to yellow fever, fevers of the Magdalena River, and malaria in the 1880s. Finally, it shows that, instead of passively incorporating bacteriology, the physicians fought to combine it with medical geography, incorporating elements of both approaches, keeping them in tense separation, or rejecting one in favor of the other.

  7. Inner ear and facial nerve complications of acute otitis media with focus on bacteriology and virology.

    Science.gov (United States)

    Hydén, Dag; Akerlind, Britt; Peebo, Markus

    2006-05-01

    Among 20 patients with inner ear complications and/or peripheral facial palsy secondary to acute otitis media (AOM) a proven or probable bacteriological cause was found in 13 (65%). In seven patients (35%), a proven or probable viral cause was found. Only two of the patients (10%), with a proven bacterial AOM and a clinical picture of a purulent labyrinthitis in both, together with a facial palsy in one, had a substantial degree of dysfunction. Although the number of patients in this study is relatively low our findings show that inner ear complications and facial palsy due to AOM can be of both bacterial and viral origin. Severe sequelae were found only where a bacterial origin was proven. Inner ear complications and/or peripheral facial palsy secondary to AOM are rare. The general understanding is that they are due to bacterial infections. However, in some of these patients there are no clinical or laboratory signs of bacterial infections and they have negative bacterial cultures. During recent years different viruses have been isolated from the middle ear or serologically proven in AOM patients and are thought to play a pathogenetic role. We suggest that in some cases of AOM complications from the inner ear and the facial nerve can be caused by viruses. The purpose of our study was to analyze infectious agents present in patients with inner ear complications and/or facial palsy arising from AOM. The medical records of 20 patients who had inner ear complications and/or facial palsy following AOM ( unilateral in 18, bilateral in 2) between January 1989 and March 2003 were evaluated. Bacterial cultures were carried out for all patients. Sera from 12 of the patients were stored and tested for a battery of specific viral antibodies. In three patients, investigated between November 2002 and March 2003, viral cultures were also performed on samples from the middle ear and nasopharynx. Nineteen patients had inner ear symptoms. Eight of them had a unilateral

  8. Bacteriological finding in the urethra in men with and without non-gonococcal urethritis

    Directory of Open Access Journals (Sweden)

    Tiodorović Jelica

    2007-01-01

    Full Text Available Background/Aim. Non-gonococcal urethritis (NGU is a very common sexually transmitted disease. The etiology of the disease is complex and not completely solved. The aim of this study was to determine the bacteriological finding in the urethra in men with and without non-gonococcal utethritis. Methods. The study group comprised 200 men with symptoms of urethritis. The control group consisted of 60 men without symptoms of urethritis. The diagnosis of nongonococcal infection was made by finding of an increased number of polymorphonuclear leukocytes (≥ 5 under the microscope in a sample of Gram-stain of urethral smear (× 1 000 and without evidence of Neisseria. gonorrhoeae in specimens (negative direct microscopy and cell culture. Bacteriological examination included: direct microscopy with the Gramstained and methylblue-stained smears of urethral discharges, and cultivation of specimens under the aerobic/unaerobic conditions. In addition to standard bacterial examination and performinig direct imunofluorescence test to detect Chlamydia trachomatis (bioMerieux, France, urethral smears were also examined for the presence of Ureaplasma urealyticum and Mycoplasma hominis by commercially available Mycofast Evolution 2 test (International Microbio, France. The finding of mycoplasmas ≥ 104 CCU/ml was positive. The data were statistically analyzed using Pearson χ2 and Student t test. Results. C. trachomatis was predominant bacterial species found in urethra in men with nongonococcal urethritis. It was isolated alone and/or mixed with mycoplasmas and/or other bacteria in 86 (43.0% of examinees. There was statistically significant difference in finding of C. trachomatis between the study group and the control group (p < 0.001. U. urealyticum was found in men with NGU: 30.2% were with C. trachomatis and 36.0% were without C. trachomatis (p > 0.05. In 16 (8.0% men with NGU, C. trachomatis was isolated alone, while in 13.0% examinees it occurred with U

  9. Qualitative bacteriology in malignant wounds- a prospective, randomized, clinical study to compare the effect of honey and silver dressings

    DEFF Research Database (Denmark)

    Lund-Nielsen, Betina; Adamsen, Lis; Gottrup, Finn

    2011-01-01

    ¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿ Between 5% and 10% of cancer patients develop malignant wounds. In vitro and some clinical studies suggest that silver- or honey-coated dressings may have an antibacterial effect in nonmalignant wounds, but their possible antibacterial effect in malignant wounds remains unknown...... antibacterial effect of the honey or silver dressing could not be confirmed in these malignant wounds. Routine wound swabbing of malignant wounds is of little value and should be restricted to cases where signs of infection requiring antibiotic intervention are observed or where resistant organisms require....... A prospective, randomized, single-blind controlled clinical study was conducted to evaluate the bacteriology of malignant wounds and compare the effect of a honey-coated (Group A) to a silver-coated (Group B) dressing on the qualitative bacteriology of malignant wounds. All wound interventions were performed...

  10. Analysis of mammary gland according to results of CMT (California Mastitis Test and bacteriological findings in secretion

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    Varatanović Nazif

    2009-01-01

    Full Text Available Health control of the mammary gland was conducted comparing results of CMT (California Mastitis Test and bacteriological findings of milk samples during one year. A total of 3863 secretion samples of mammary glands were collected. There were 85.3% matches for CMT and bacteriological findings. The most frequently isolated causative agents were: staphylococci (52.4%, streptococcus bacteria (23.5%, mixed infections (13.1%, and enterobacteria (10.3%. Secretions disorder and inflammations of mammary gland are most common for the warm period of the year, and very rare for the winter period. With permanent use of these two methods it is possible to obtain satisfactory results in order to get better milk production and health condition of the mammary gland. .

  11. The Bacteriological safety of air movements and air changes in operating theatres. A technique for continuous monitoring

    NARCIS (Netherlands)

    Wiegersma, Nicolaas

    1980-01-01

    Het risico van wondbesmetting tijdens operaties is afhankelijk van een groot aantal factoren. In dit proefschrift wordt een aantal van deze factoren onderzocht met als kernpunt de invloed van het toegepaste ventilatiesysteem op het besmettingsrisico van de wond, de instrumenten en de wondomgeving

  12. [Bacteriology of community acquired meningitis in Sfax, Tunisia (1993-2001)].

    Science.gov (United States)

    Maalej, S Mezghani; Kassis, M; Rhimi, F Mahjoubi; Damak, J; Hammami, A

    2006-02-01

    The aim of this study was to analyze the distribution of bacteria responsible for community-acquired meningitis and the pattern of resistance of common species. All bacteriologically confirmed cases of community-acquired meningitis were recorded between 1993 and 2001. Two hundred twenty-four cases of bacterial meningitis were recorded. The most frequent species were Haemophilus influenzae and Streptococcus pneumoniae followed by Neisseria meningitidis with respectively 37.1%, 32.1%, and 10.7% of cases. The yearly distribution of these bacteria did not show any epidemic peak. Enterobacteria and group B Streptococcus were the most frequently identified pathogens in neonatal meningitis. H. influenzae was the predominant microorganism in children between one month and five years of age, (66.4%) followed by S. pneumoniae (23.5%). S. pneumoniae was the predominant bacteria responsible fore more than half of the cases over five years of age. 28.8% of H. influenzae strains produced beta-lactamase. 27.2% of S. pneumoniae strains were less susceptible to penicillin. Resistance rates for amoxicillin and cefotaxime were respectively 10.6% and 7.5%. Only one strain of N. meningitidis (4.2%) presented with a decreased susceptibility to penicillin. In our study, H. influenzae and S. pneumoniae were the main microorganisms responsible for community-acquired meningitis. High resistance rates were found for these bacteria: 28.8% of H. influenzae to ampicillin and 27.2% of S. pneumoniae to penicillin.

  13. Prevalence of Taylorella equigenitalis infection in stallions in Slovenia: bacteriology compared with PCR examination.

    Science.gov (United States)

    Zdovc, I; Ocepek, M; Gruntar, I; Pate, M; Klobucar, I; Krt, B

    2005-05-01

    The prevalence of Taylorella equigenitalis infection in Slovenia is unknown and methods used to refine identification in these stallions are required. In diagnosis of T. equigenitalis, polymerase chain reaction (PCR) would have advantages over culture methods, especially in cases where small numbers of causal agent or intensive contamination of genital swabs are involved. Culture method and PCR were used to examine a total of 980 genital swabs from the urethra and fossa urethralis of 245 stallions for the presence of the contagious equine metritis organism. Among 245 examined stallions, 225 (91.8%) were negative to T. equigenitalis by both methods. From the swabs of 17 stallions (6.9%) T. equigenitalis was isolated at first and/or second sampling. Swabs of 3 (13%) stallions were PCR positive but the isolation of T. equigenitalis failed. The rate of T. equigenitalis detection was higher with PCR than with the classic bacteriological examination. PCR protocol used in this study provided a specific, sensitive, and simple tool for rapid detection of T. equigenitalis. PCR is especially valuable in cases of intensive bacterial and fungal contamination of swabs where the isolation of T. equigenitalis usually fails.

  14. Travel grant program for the IX International Congresses of Mycology and Bacteriology -- Final report

    Energy Technology Data Exchange (ETDEWEB)

    Granigan, Marion

    2000-05-25

    In 1999, the American Society for Microbiology (ASM) and the National Academy of Sciences' U.S. National Committee for the International Union of Microbiological Sciences (IUMS) jointly organized a competitive travel grant program to support the participation of U.S. scientists in the 9th International Congresses of the Bacteriological and Applied Microbiology, Mycology and Virology Divisions of the IUMS in Sydney, Australia, August 16-20, 1999. Funding was solicited for the program, and the ASM Minority and International Activities department administered the $40,000 raised. Travel grants in the amount of $2,000 were offered to U.S. investigators (citizens, including federal employees, and permanent residents working in the United States) in the early stages of their careers who planned to attend and present their research at the Congress. Teams of established and new investigators who applied jointly were eligible to received a combined $3,000 award. IUMS developed a questionnaire th at each applicant were required to complete and return, which asked each award recipient about their experience at the Congresses. Questionnaire results are included.

  15. BACTERIOLOGICAL PROPERTIES OF MARINE WATER IN ADRIATIC FISH FARMS: ENUMERATION OF HETEROTROPHIC BACTERIA

    Directory of Open Access Journals (Sweden)

    Emin Teskeredžić

    2012-12-01

    Full Text Available Aquaculture is currently one of the fastest growing food production sectors in the world. Increase in nutrients and organic wastes lead to general deterioration of water quality. The problem of water quality is associated with both physical and chemical factors, as well as microbiological water quality. Heterotrophic bacteria play an important role in the process of decomposition of organic matter in water environment and indicate eutrophication process. Here we present our experience and knowledge on bacterial properties of marine water in the Adriatic fish farms with European sea bass (Dicentrarchus labrax L., 1758, with an emphasis on enumeration of heterotrophic bacteria in marine water. We applied two temperatures of incubation, as well as two methods for enumeration of heterotrophic bacteria: substrate SimPlate® test and spread plate method on conventional artificial media (Marine agar and Tryptic Soy agar with added NaCl. The results of analysis of bacteriological properties of marine water in the Adriatic fish farms showed that enumeration of heterotrophic bacteria in marine water depends on the applied incubation temperature and media for enumeration. At the same time, the incubation temperature of 22C favours more intense growth of marine heterotrophic bacteria, whereas a SimPlate test gives higher values of heterotrophic bacteria. Volatile values of heterotrophic bacteria during this research indicate a possible deterioration of microbiological water quality in the Adriatic fish farms and a need for regular monitoring of marine water quality.

  16. Bacteriological assessment of the hospital environment in two referral hospitals in Yaoundé-Cameroon.

    Science.gov (United States)

    Gonsu, Kamga Hortense; Guenou, Etienne; Toukam, Michel; Ndze, Valantine Ngum; Mbakop, Calixte Didier; Tankeu, Dongmo Norbert; Mbopi-Keou, Francois Xavier; Takongmo, Samuel

    2015-01-01

    Many studies still show significant numbers of surgical patients contracting nosocomial infections each year globally with high morbidity and mortality. The aim of this study was to identify potential bacteria reservoirs that may be responsible for nosocomial infection in surgical services in the Yaoundé University Teaching Hospital (YUTH) and the Central Hospital Yaoundé (CHY). A cross sectional descriptive study was conducted from June to August 2012. Air, water, and surface samples were collected from two surgical services and subjected to standard bacteriological analysis. A total of 143 surface samples were collected. Bacteria were isolated in all surfaces except from one trolley sample and a surgical cabinet sample. The predominant species in all services was coagulase negative Staphylococcus (CNS). The average number of colonies was 132. 82 CFU/25 cm(2). The bacteria isolated in the air were similar to those isolated from surfaces. From the 16 water samples cultured, an average of 50.93 CFU/100ml bacteria were isolated. The distribution of isolated species showed a predominance of Burkholderia cepacia. These results showed the importance of the hospital environment as a potential reservoir and source of nosocomial infections amongst surgical patient at YUTH and CHY, thus we suggest that Public health policy makers in Cameroon must define, publish guidelines and recommendations for monitoring environmental microbiota in health facilities.

  17. [Bacteriological evaluation of a procedure for disinfecting the Olympus GIF-D2 panendoscope].

    Science.gov (United States)

    Ramírez Ramos, A; Domínguez, N; Makino, R; Barrera, C

    1980-01-01

    We have performed a total of 107 cultures from three critical areas of an Olympus Panendoscope Model GIF-D2 in order to evaluate bacteriologically cur system of desinfection of this endoscope. Samples were taken from the distal end, external surface and biopsy canal before and after an endoscopic examination was performed. The procedure of desinfection employed was as follows: washing of the distal end, external surface and biopsy canal with Hexaclorophel (Phisohex) diluted 50% with water and a second washing with tap water. In the middle of the study, we added a second washing of the biopsy canal with ten ml. of ether alcohol to allow for better drying. As a result of the present study we observed that in the distal end in 50% of the samples we encountered bacteria. Cultures of the external surface were positive in 20% of samples. The biopsy canal should be washed with ether alcohol to allow for complete drying, because when we did not use this method, Pseudomonas Aeruginosa was isolated. After this modification we did not isolate bacteria. The most frequent types of isolated bacteria were from the normal oropharyngeal flora. From the present study we can conclude that desinfection of the Panendespe with Hexaclorophen gives satisfactory results on the external surface of the endoscope. Biopsy canal requires additional washing with ether alcohol. However, both procedures do not assure a satisfactory desinfection of the distal end.

  18. Bacteraemia in Intensive Care Unit: Clinical, Bacteriological, and Prognostic Prospective Study

    Directory of Open Access Journals (Sweden)

    Zineb Lachhab

    2017-01-01

    Full Text Available Objectives. We conducted a one-year observational study from December 2012 to November 2013 to describe the epidemiology of bacteraemia in intensive care units (ICU of Mohammed V Military Teaching Hospital of Rabat (Morocco. Methods. The study consisted of monitoring all blood cultures coming from intensive care units and studying the bacteriological profile of positive blood cultures as well as their clinical significance. Results. During this period, a total of 46 episodes of bacteraemia occurred, which corresponds to a rate of 15,4/1000 patients. The rate of nosocomial infections was 97% versus 3% for community infections. The most common source of bacteraemia was the lungs in 33%, but no source was identified in 52% of the episodes. Gram negative organisms were isolated in 83,6% of the cases with Acinetobacter baumannii being the most frequent. Antibiotic resistance was very high with 42,5% of extended-spectrum beta-lactamases (ESBLs in Enterobacteriaceae and 100% of carbapenemase in Acinetobacter baumannii. The antibiotherapy introduced in the first 24 hours was adequate in 72% of the cases. Conclusions. Bloodstream infections in ICU occur most often in patients over 55 years, with hypertension and diabetes. The bacteria involved are mainly Gram negative bacteria multiresistant to antibiotics. Early administration of antibiotics significantly reduces patients mortality.

  19. Analysis of physico-chemical and bacteriological quality of drinking water in Mafikeng, South Africa.

    Science.gov (United States)

    Mulamattathil, Suma George; Bezuidenhout, Carlos; Mbewe, Moses

    2015-12-01

    Mafikeng, the capital of the North West Province, receives water from two sources, namely the Molopo eye and the Modimola dam. Once treated, the potable water is mixed and supplied to the city via distribution systems. This study was designed to assess the quality of drinking water in Mafikeng and also to determine whether the water from the two sources has an impact on the mixed water quality. Physico-chemical parameters and bacteriological quality (faecal coliforms (FCs), total coliforms (TCs), heterotrophic bacteria and Peudomonas spp.) was monitored at three drinking water sites weekly for 4 months. The results revealed that the physico-chemical quality of the water was generally acceptable. The pH ranged from 5.7 ± 0.18 to 8.6 ± 0.14, the temperature ranged from 18.3 ± 0.69 to 25.1 ± 0.69 °C and the total dissolved solids (TDS) ranged from 159.9 ± 22.44 to 364.4 ± 12.44 mg/l. These values are within the target water quality range for drinking water as prescribed by WHO, Department of Water Affairs and SANS 241. What is of concern was the microbial quality of the water. FCs, TCs, heterotrophic bacteria and Pseudomonas spp. were present in some of the treated water samples. The most significant finding of this study is that all drinking water samples were positive for Pseudomonas spp. (>100/100 ml).

  20. Bacteriological and parasitological assessment of food handlers in the Omdurman area of Sudan.

    Science.gov (United States)

    Saeed, Humodi Ahmed; Hamid, Hatim Hassan

    2010-02-01

    Pathogenic organisms are thought to be widely distributed among food handlers. This study was designed to assess the prevalence of carriers of some pathogenic bacteria and intestinal parasites among food handlers in the city of Omdurman, Sudan. A total of 518 nasal swabs and stool specimens were collected. Nasal swabs were cultured on bacteriological culture media. Stool specimens were examined microscopically for intestinal parasites. Of the total subjects examined, 30.1% were found to be carriers of pathogenic organisms. The pathogens isolated and identified were the bacteria Staphylococcus aureus, Salmonella typhi, and Shigella boydii, and the intestinal parasites, Giardia lamblia and Entamoeba histolytica/dispar. Bacteria and intestinal parasites were most prevalent among storekeepers (41%), followed by restaurant workers (24.4%), bakers (24.4%), butchers (5.1%), milk distributors (2.6%), and fruits/vegetables sellers (2.6%). S. aureus, the most abundant pathogen, was most prevalent in storekeepers (44.6%), followed by restaurant workers (25%), bakers (17.9%), butchers (5.4%), milk distributors (3.6%), and fruit/vegetable sellers (3.6%). The findings from this study indicate a key role for food handlers in the spread and transmission of food communicable diseases and reveal the need for protective measures.

  1. Bacteriological and mycological surveys along Otranto coastal areas; La situazione igienica del litorale salentino

    Energy Technology Data Exchange (ETDEWEB)

    Montagna, M.T. [Bari Univ., Bari (Italy). Ist. di Igiene; De Donno, A.; Bagordo, F. [Lecce Univ., Lecce (Italy). Dipt. di Biologia, Lab. di Igiene; Carrozzini, F. [ASL LE/2, Lecce (Italy). Direzione Sanitaria

    2000-01-01

    Bacteriological and mycological surveys were carried out on water samples collected from 6 seaside resorts and 2 affluent along Otranto coastal areas. The following parameters were tested: total coliforms, fecal coliforms, fecal streptococci, Salmonella, P. aeruginosa and S. aureus, Vibrio species and yeasts. Results show fecal pollution in three seaside resorts and in one drain, whereas Salmonella research always resulted negative. P. aeruginosa and S. aureus were isolated from both affluents. V. alginolyticus was the most isolated vibrio specie (90%). Among isolated yeasts Torulopsis inconspicua (70%) was the most frequent one. [Italian] E' stata condotta una indagine batteriologica e micologica su campioni di acqua provenienti da 6 stabilimenti balneari e da 2 affluenti che sfociano nel litorale di Otranto. Sono stati esaminati i seguenti parametri: coliformi totali, coliformi fecali, streptococchi fecali, salmonelle, P. aeruginosa, S. aureus, vibrioni e miceti. Dall'indagine e' emerso che indici di contaminazione fecale sono stati evidenziati in tre stazioni balneari e in un affluente mentre la ricerca delle salmonelle e' risultata sempre negativa. P. aeruginosa e S. aureus sono stati isolati in entrambi gli affluenti. Per quanto riguarda la ricerca dei vibrioni, V. alginolyticus e' apparsa la specie piu' frequente (90%). Tra i lieviti isolati, quello piu' frequente e' risultato Torulopsis inconspicua (70%).

  2. Fusobacterium nucleatum infections: clinical spectrum and bacteriological features of 78 cases.

    Science.gov (United States)

    Denes, E; Barraud, O

    2016-08-01

    Few series describe the clinical spectrum of Fusobacterium spp. infections. Among them, fewer discuss F. nucleatum, even though there are many clinical cases. We performed a retrospective study over 8 years (from 2007 to 2014) in Limoges University Hospital, France, to assess clinical and bacteriological aspects of infections due to F. nucleatum. Eighty-one patients with F. nucleatum positive cultures were included in this study, irrespective of sample origin. Abscesses (n = 43), bacteraemia (n = 18) and bone infections (n = 8) were the most common types of infections, Abscesses were found in various organs (mostly skin, brain, pleura, liver). Co-morbidities were found in 38 patients (47 %) with neoplasia, diabetes, and alcoholism and history of smoking. There were more neoplasms in patients with bacteraemia than in patients with abscesses (p = 0.007). In 51 cases (65.4 %), infection was polymicrobial, either during bacteraemia or abscesses. Main associations were with Streptococcus spp., Peptostreptococcus spp. and/or Prevotella spp. The sources of infection, when found, were either dental or gastrointestinal. All isolates were susceptible to penicillin, clindamycin and metronidazole. Infections involving F. nucleatum are uncommon and potentially severe, with many abscesses requiring surgery. Bacteraemia was mainly associated with co-morbidities such as cancer. Polymicrobial infections were very common and there is probably interaction and/or synergy between F. nucleatum and some other commensal bacteria to cause infections and abscesses.

  3. Experimental staphylococcal mastitis in bitches: clinical, bacteriological, cytological, haematological and pathological features.

    Science.gov (United States)

    Ververidis, H N; Mavrogianni, V S; Fragkou, I A; Orfanou, D C; Gougoulis, D A; Tzivara, A; Gouletsou, P G; Athanasiou, L; Boscos, C M; Fthenakis, G C

    2007-09-20

    The objectives of the work were to study the features of experimentally induced canine mastitis and to present hypotheses regarding the pathogenesis of the disease. The right caudal abdominal mammary gland of six bitches was inoculated on day 8 after whelping with Staphylococcus intermedius to induce mastitis; adjacent mammary glands were used as controls. Clinical examination, bacteriological and cytological (whiteside test, Giemsa) examination of mammary secretion, as well as haematological tests were performed from 5 days before until 34 days after challenge. Mastectomy was sequentially performed 1, 2, 4, 18, 26 and 34 days after challenge in each of the bitches, in order to carry out a pathological examination of mammary glands. All animals developed clinical mastitis: challenged glands became painful, hot, enlarged and oedematous; secretion was brownish, purulent, with flakes or clots, subsequently becoming yellowish and thick. Staphylococci were isolated from all inoculated glands (up to 22 days). WST was positive in 41/46 samples from inoculated glands and 66/138 samples from control glands; neutrophils predominated during the acute stage. Blood leukocyte counts increased, whilst platelet counts decreased. Gross pathological findings initially included congestion, purulent discharge and subcutaneous oedema; then abscesses, brownish areas and size decrease were seen. Salient histopathological features were initially neutrophilic infiltration, haemorrhages, destruction of mammary epithelial cells and alveoli, and then infiltration by lymphocytes, shrunken alveoli, loss of glandular architecture and fibrous tissue proliferation. We conclude that in bitches, intrammamary inoculation of Staphylococcus intermedius can induce clinical mastitis, followed by subclinical disease. The disorder is characterized by bacterial isolation and leukocyte influx in challenged glands, by leukocyte presence in adjacent mammary glands, by increased blood leukocyte counts and by

  4. Bacteriological features of infectious spondylodiscitis at Mohammed V Military Teaching Hospital of Rabat

    Directory of Open Access Journals (Sweden)

    A Zohoun

    2012-12-01

    Full Text Available To review the bacteriological features of infectious spondylodiscitis and provide recommendations for the initial therapy which remains empirical in our context. Retrospective study including patients diagnosed with spondylodiscitis over a period of 4 years (2006-2009 at the Rabat Military Teaching Hospital. During the study period, we analysed 30 cases: the mean age was 49.9 years and 21 cases (70% were male. The patients were predominantly hospitalized in neurosurgery department (15/30 followed by rheumatology department (10/30. The site of infection was lumbar in 21 cases (21/30, dorsal in 7 cases (7/30. 26 cultures were positive of which 19 (19/26 were monomicrobial. Tuberculosis (TB was implicated in 10 cases (10/30 including 4 cases in association with common organisms (Propionibacterium acnes, Staphylococcus aureus, Staphylococcus epidermidis, Corynebacterium species. Brucella melitensis was isolated in 1 case. Infections caused by pyogenic bacteria were isolated in 15 cases of which 12 (12/15 revealed simple organisms including Gram-positive cocci in 9 cases (9/12 with 3 cases of S. aureus and Gram-negative bacilli in 3 cases (3/12 with 2 cases of P. aeruginosa. Blood cultures carried out for 16 patients were positive in 7 cases. The anatomopathologic exams carried out for 20 patients found in 6 cases epithelioid granulomata and giants cells with caseous necrosis in total concordance with TB culture. TB is the most frequent cause of spondylodiscitis in Morocco. Our study found the same frequency for non-specific and specific germs. Empirical treatment must take into account S. aureus and M. tuberculosis.

  5. BACTERIOLOGY AND RISK FACTORS OF BACTERIAL KERATITIS IN JIMMA, SOUTHWEST ETHIOPIA.

    Science.gov (United States)

    Gebremariam, Tewelde Tesfaye

    2015-10-01

    In East Africa, particularly in Ethiopia, bacterial keratitis is a major cause of blindness. The aims of this study were to identify risk factors of bacterial keratitis and the spectrum of bacterial etiologies, and to assess the in-vitro antimicrobial susceptibility of these bacterial isolates at Jimma University Specialized Hospital, Southwest Ethiopia. A prospective study was employed from January 2012 to June 2012 from which a total of 24 patients with bacterial keratitis were included in the study. Corneal scrapings were collected, transported and microbiologically processed using standard operating procedures. Four different predisposing factors for bacterial keratitis were identified: corneal trauma 9 (37.5%), blepharitis 7 (29.2%), herpetic keratitis, and use of contaminated medications 4. Bilateral corneal infection was found in 5 (20.8%) of the cases. A total of 24 corneal scrapings were collected for microbiological evaluation, of which 20 (83%) had bacterial growth. The isolated bacterial pathogens were Pseudomonas aeruginosa 10 (41.7%), Staphyloccus aureus 5 (20.8%), Serratia marcescens 3 (12.5%), followed by Streptococcus pneumoniae 2 (8.3%). Antimicrobial susceptibility testing revealed that 85% of Gram-negative bacilli were susceptible to gentamicin and ciprofloxacin, while 86% of Gram-positive cocci were susceptible to vancomycin and Ciprofloxacin. Corneal trauma was the most common risk factor for bacterial keratitis followed by blepharitis. Bacteriological analysis of corneal scrapings also revealed that P. aeruginosa was the most common isolate followed by S. aureus; the antibiotic with the highest susceptibility was ciprofloxacin. As drug resistance among bacterial pathogens is an evolving process, routine surveillance and monitoring studies should be conducted to provide an update and most effective empirical treatment for bacterial keratitis.

  6. Assessment of the physicochemical and bacteriological qualities of Nono – a fermented cow milk

    Directory of Open Access Journals (Sweden)

    Pius Abimbola Okiki

    2018-02-01

    Full Text Available Nono is a spontaneously fermented yoghurt-like milk product consumed is a staple food commodity in parts of the Sub-Saharan West Africa. Nono is usually consumed along with 'Fura' as 'Fura da Nono' in Nigeria. Studies on physicochemical and bacteriological qualities were carried out on samples of Nono obtained from 5 different sources in Ado-Ekiti, Nigeria. The Nono samples were found to be nutritious, containing moderate levels of ash, crude fat, crude protein and carbohydrate. The pH of the Nono samples was relatively low (4.04 ±0.04, while the density and specific density were close to that of distilled water at room temperature. Total aerobic plate count of Nono samples was 1.8 ±0.02 × 106 CFU.mL-1. A total of 15 bacteria species namely Eubacterium nodatum, Bacillus subtilis, Chromobacterium violaceum, Propionibacterium acnes, Amycolatopsis benzotilytica, Tropheryma whipplei, Moraxella catarrhalis, Campylobacter gracilis, Neisseria sicca, Vibrio natiensis, Photobacterium damselae, Corynebacterium kutsceri, Corynebacterium xerosis, Lactobacillus fermentum and Lactobacillus casei were isolated from the Nono samples. The gram-positive bacterial isolates were resistant to all antibiotics tested with the exception of Erythromycin where 40% susceptibility was obtained, while the gram-negative bacteria showed high resistance to the tested antibiotics, but with 80% susceptibility to Ofloxacin. The nono samples were observed to exhibit antibacterial activity against cultures of Salmonella typhimurium ATCC 14028, Escherichia coli ATCC 29929 and Staphylococcus aureus ATCC 29293. Most of the bacteria isolated were of less public health importance, but the high prevalence of multi-drug resistance is of great concern.

  7. Clinical mastitis in dairy cattle in Ontario: frequency of occurrence and bacteriological isolates.

    Science.gov (United States)

    Sargeant, J M; Scott, H M; Leslie, K E; Ireland, M J; Bashiri, A

    1998-01-01

    The objective of this study was to describe the frequency of occurrence of clinical mastitis in dairy herds in Ontario. The study group consisted of 65 dairy farms involved in a 2-year observational study, which included recording all clinical mastitis cases and milk sampling of quarters with clinical mastitis. Lactational incidence risks of 9.8% for abnormal milk only, 8.2% for abnormal milk with a hard or swollen udder, and 4.4% for abnormal milk plus systemic signs of illness related to mastitis were calculated for 2840 cows and heifers. Overall, 19.8% of cows experienced one or more cases of clinical mastitis during location. Teat injuries occurred in 2.1% of lactations. Standard bacteriology was performed on pretreatment milk samples from 834 cows with clinical mastitis. The bacteria isolated were Staphylococcus aureus (6.7%), Streptococcus agalactiae (0.7%), other Streptococcus spp. (14.1%), coliforms (17.2%), gram-positive bacilli (5.5%), Corynebacterium bovis (1.7%), and other Staphylococcus spp. (28.7%). There was no growth in 17.7% of samples, and 8.3% of samples were contaminated. Clinical mastitis is a common disease in dairy cows in Ontario; approximately 1 in 5 cow lactations have at lease one episode of clinical mastitis. There is, however, considerable variation in the incidence of clinical mastitis among farms. The majority of 1st cases of clinical mastitis occur early in lactation, and the risk of clinical mastitis increases with increasing parity. Environmental, contagious, and minor pathogens were all associated with cases of clinical mastitis.

  8. CHRONIC OSTEOMYELITIS: A BACTERIOLOGICAL STUDY WITH SPECIAL REFERENCE TO STAPHYLOCOCCUS AUREUS

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    Sanjoy Chakravarty

    2015-01-01

    Full Text Available Advances in the identification of infections and early diagnosis of Osteomyelitis have led to the improved management of Osteomyelitis. This study was undertaken to determine the bacteriological profile of Osteomyelitis and the antibiotic resistance pattern of various isolates obtained as it is an important cause of morbidity. A total of 50 patients of Osteomyelitis either attending the outpatient department or admitted in the wards of a teaching and tertiary care hospita l in Sikkim from October 2013 to October, 2014 were included in the study. All those patients who were clinically and/ or radiologically suspected of having Osteomyelitis were enrolled as cases. Pus/ pus swabs or sequestrum samples taken aseptically were c ultured aerobically at 37 0 C for 18 - 24 hours in Blood and Mac Conkey agar plates. Culture isolates were identified by a series of standard biochemical reactions. Antibiotic susceptibility was tested on Mueller Hinton agar by Kirby Bauer disc diffusion met hod. Betalactamase production of S. aureus strains were verified by iodometric filter paper and acidometric agar plate methods. S. aureus strains were screened for methicillin resistance by using conventional microbiological methods. S. aureus turned out t o be the most common organism isolated. Other organism isolated were P. Aeruginosa, Proteus spp., Klebsiella spp., E. coli, Enterobacter spp., S. epidermitis, Streptococcus pyogens and Enterococcus spp. Beta - lactamase production and methicillin resistance was seen in S. aureus strains respectively. Multidrug resistance was observed in other strains. Infection caused by Methicillin resistant S. aureus and multidrug resistant organisms are posing a major challenge in the treatment of Osteomyelitis. So, appropriate drug selected by antibiotic sensitivity testing should be used to treat Osteomyelitis

  9. Research on Handwashing Techniques of Peritoneal Dialysis Patients From Yiwu, Southeast China.

    Science.gov (United States)

    Ye, Yuanjun; Zhang, Xiaohui; Liu, Yansu; Lou, Hongqing; Shou, Zhangfei

    2017-04-01

    The aim of the present study was to evaluate handwashing technique, bacteriology, and factors influencing handwashing technique of 86 stable chronic peritoneal dialysis (PD) patients from Yiwu City in Southeast China. Based on the "Hygienic standard for disinfection in the hospital", we also performed sampling for bacteriology from PD operators after they washed their hands. We compared their clinical features including the pathogenic bacteria of their previous peritonitis episodes and their handwashing evaluation results according to their bacteriologic sampling results. 65% of patients turned off the tap by bare hand, and 74% did not follow the six-step handwashing method. Dialysis duration longer than 6 months (P = 0.04) and lower income (P = 0.05) were independent risk factors for higher handwashing error scores. The overall rate of appropriate handwashing, according to the "hygienic standard for disinfection in the hospital" was 26%. The bacteriologic sampling results showed that the most common pathogenic bacterium was Staphylococcus aureus (92%). PD operators whose hand bacteria culture was qualified contained a lower proportion of participants with advanced age (P = 0.07). Patients with repeated peritonitis occurrence had a significantly higher score on handwashing error (P culture (Staphylococcus) from their hands. © 2017 International Society for Apheresis, Japanese Society for Apheresis, and Japanese Society for Dialysis Therapy.

  10. Changing values of lake ecosystem services as a result of bacteriological contamination on Lake Trzesiecko and Lake Wielimie, Poland

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    Cichoń Małgorzata

    2017-12-01

    Full Text Available Lake ecosystems, on the one hand, are affected by tourism, and on the other by development for tourism. Lake ecosystem services include: water with its self-cleaning processes, air with climate control processes, as well as flora and fauna. Utilisation of services leads to interventions in the structure of ecosystems and their processes. Only to a certain extent, this is specific to each type of environmental interference, remains within the limits of ecosystem resilience and does not lead to its degradation. One of the threats is bacteriological contamination, for which the most reliable sanitation indicator is Escherichia coli. In lake water quality studies it is assumed that the lakeshore cannot be a source of bacteria. It has been hypothesised that the problem of bacterial contamination can be serious for the places that do not have any infrastructure, especially sanitation. Consequently, the purpose of the study was to determine the extent to which lakeshore sanitation, in particular the level of bacteriological contamination, has an impact on the value of services provided by the selected lake ecosystems (Lake Trzesiecko and Lake Wielimie – Szczecinek Lake District. Five selected services related to lake ecosystems are: water, control over the spread of contagious diseases, aesthetic values, tourism and recreation, as well as the hydrological cycle with its self-cleaning function. Services, as well as the criteria adopted for evaluation, allow us to conclude that the services provided by the lake ecosystems are suitable to fulfill a recreation function. However, the inclusion of quality criteria for sanitary status has shown that the value of system services has dropped by as much as 50%. Value changes are visible primarily for water and aesthetic qualities. Such a significant decrease in the value of services clearly indicates the importance of the sanitary conditions of lakes and their appropriate infrastructure. In view of the

  11. Open Defaecation and Its Effects on the Bacteriological Quality of Drinking Water Sources in Isiolo County, Kenya

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    Joab Odhiambo Okullo

    2017-10-01

    Full Text Available Background information: The post-2015 Sustainable Development Goals for sanitation call for universal access to adequate and equitable sanitation and an end to open defaecation by 2030. In Isiolo County, a semi-arid region lying in the northern part of Kenya, poor sanitation and water shortage remain a major problem facing the rural communities. Objective: The overall aim of the study was to assess the relationship between sanitation practices and the bacteriological quality of drinking water sources. The study also assessed the risk factors contributing to open defaecation in the rural environments of the study area. Methods: A cross-sectional study of 150 households was conducted to assess the faecal disposal practices in open defaecation free (ODF and open defaecation not free (ODNF areas. Sanitary surveys and bacteriological analyses were conducted for selected community water sources to identify faecal pollution sources, contamination pathways, and contributory factors. Analysis of data was performed using SPSS (descriptive and inferential statistics at α = .05 level of significance. Results: Open defaecation habit was reported in 51% of the study households in ODNF villages and in 17% households in ODF villages. Higher mean colony counts were recorded for water samples from ODNF areas 2.0, 7.8, 5.3, and 7.0 (×10 3 colony-forming units (CFUs/100 mL compared with those of ODF 1.8, 6.4, 3.5, and 6.1 (×10 3 areas for Escherichia coli , faecal streptococci, Salmonella typhi , and total coliform, respectively. Correlation tests revealed a significant relationship between sanitary surveys and contamination of water sources ( P  = .002. Conclusions: The water sources exhibited high levels of contamination with microbial pathogens attributed to poor sanitation. Practising safe faecal disposal in particular is recommended as this will considerably reverse the situation and thus lead to improved human health.

  12. The bacteriological quality of different brands of bottled water available to consumers in Ile-Ife, south-western Nigeria.

    Science.gov (United States)

    Igbeneghu, Oluwatoyin A; Lamikanra, Adebayo

    2014-11-28

    The upsurge in the demand for bottled water has prompted the interest of many manufacturers in the production of bottled water and very many water bottling companies are therefore involved in its production. These range from large scale multinational companies to medium scale business enterprises, institutional and government business investment companies as well as small scale entrepreneurs. There is however little information on the comparative quality of bottled water brands produced by different classes of water bottling companies in Nigeria. This study was undertaken to determine the bacteriological quality of brands of bottled water available to consumers in Ile-Ife. Forty-three samples of bottled water comprising of three batches each of thirteen bottled water brands and two batches of two brands were purchased and analyzed for total bacterial count, presence of coliform and the presence of other bacterial indicators of drinking water quality. Only 67.4% of the water samples representing the products of 10 companies or 66.7% of the brands had heterotrophic counts within the acceptable limits. Coliforms present in 100 ml of water were detected in 26.7% of the bottled water brands. Other indicator organisms detected included Staphylococci isolated from 27.9% of the samples (33.3% of the brands) and specifically Staphylococcus aureus found in four brands constituting 14% of the samples. Pseudomonas strains were consistently detected in consecutive batches of three brands of the water samples. Bottled water samples produced by the large scale multinational producers were of acceptable bacteriological quality unlike those produced by most small companies. There is need for a greater control of water bottling processes carried out by commercial bottled water producers in Nigeria.

  13. Bacteriology data from moored buoy casts and other instruments in the Delaware Bay and North Atlantic Ocean during the Ocean Continental Shelf (OCS-Mid Atlantic Ocean) project, 1976-11-05 to 1977-08-16 (NODC Accession 7800207)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Bacteriology data were collected using moored buoy casts and other instruments in the Delaware Bay and North Atlantic Ocean from November 5, 1976 to August 16, 1977....

  14. Bacteriology, wind wave spectra, and benthic organism data from moored buoy casts and other instruments in the Gulf of Mexico during the Brine Disposal project, 1978-02-01 to 1979-05-03 (NODC Accession 7900247)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Bacteriology, wind wave spectra, and benthic organism data were collected using moored buoy casts and other instruments in the Gulf of Mexico from February 1, 1978...

  15. UK and European Union public and charitable funding from 2008 to 2013 for bacteriology and antibiotic research in the UK: an observational study.

    Science.gov (United States)

    Bragginton, Eilis C; Piddock, Laura J V

    2014-09-01

    Since the 1990s, the number of new antibacterial drugs has plummeted and the number of antibiotic-resistant infections has risen, which has decreased the effective treatment of many disorders, including sepsis. We aimed to assess whether funding for bacteriology and antibiotic research to UK researchers had increased in response to this global crisis. We systematically searched websites and databases of agencies that fund research in the UK to identify publicly and charitably funded projects from financial years 2008 to 2013 within the specialties of bacteriology and antibiotic research. We created a database to identify the projects funded. Grants awarded in euros were converted to pounds sterling (€1=£0·86). We identified 609 projects within the specialty of bacteriology, 196 (32·2%) of which were on antibiotics. Of £13 846·1 million of available research funding, £269·2 million (1·9%) was awarded to bacteriology projects and £95·0 million (0·7%) was awarded for research on antibiotics. Additionally, £181·4 million in European Union (EU) funding was awarded to antibiotic research consortia including researchers based within the UK, including two EU Innovative Medicines Initiative awards, totalling £85·2 million. To increase awareness of who funds antibiotic research and to facilitate priority setting and funding decisions, funding organisations need to be aware of the breadth and depth of present funding as a baseline by which funding from 2014 onwards can be measured and so that informed decisions about the future level of funding can be made. To resolve the crisis of antibiotic resistance, present levels of funding are inadequate and should be increased substantially. British Society for Antimicrobial Chemotherapy. Copyright © 2014 Elsevier Ltd. All rights reserved.

  16. A contribution to the bacteriological and hygienic risk assessment of raw milk and raw milk products from farm marketing with emphasis on pathogenic bacteria

    OpenAIRE

    Coenen, Claudia

    2010-01-01

    During the last years farm marketing has significantly increased. The present study deals with the bacteriological-hygienic quality, particularly with pathogenic microorganisms of raw milk and raw milk products from farm marketing. 709 samples of raw milk and raw milk products were collected nationwide. Certified milk (a kind of raw certified grade A milk), ex-farm milk, raw milk cheeses (soft cheese, semihard and hard cheese) and other products made of raw milk underwent quality checks for L...

  17. [Bacteriological control of blood preservation, production of infusion solutions and dry human plasma under conditions of aseptic work and possible sources of their contamination].

    Science.gov (United States)

    Bosković, S; Lucić, N; Aganović, N; Grbić, E

    1975-01-01

    In premises for blood conservation, production of dry human plasma and infusion solutions "notwithstanding the permanent measures for desinfection, new bacterial contamination occurs from time to time and whose source are the casings and material originating from non-sterile environment. Bacteriological control, which has primarily a preventive character, enables a due forecast for measures to be undertaken by the appropriate desinfection of the working surfaces and air, satisfactory conditions of aseptic work can be maintained. General hygiene should be paid attention to as well as mechanical cleansing of premises, avoidance of groups for lunch-time etc., since the treatment by desinfectors would not be sufficient for maintenance of aseptic working conditions. In order to prevent the transmission of bacterial contamination, premises for blood conservation should be strictly separated from other operations and also prevent the unnecessary movements of personnel through corridors. The results of the bacteriological control of the personnel show that greater attention should be paid to their health care since the workers there work in closed aseptic systems and thus avoid them as a bacteria transmittors in respect to danger of blood and dry human plasma contamination. It is also necessary to efficiently educate the personnel for work in aseptic conditions and also increase their elementary knowledge from bacteriology and hygiene. The bacterial skin-flora on the spot of donor's venepuncture also presents a certain danger for blood contamination. Therefore, it is necessary to establish the most optimal manner of skin desinfecate together with the most appropriate means having a fast bactericidal and fungicidal action. It would also be useful, on the basis of further test, to suggest certain standard for an allowed number of conditionally pathogenic and saprophytic microorganisms which would be used by the instutions performing the blood transfusion and production of

  18. Bacteriological quality of the wastewater used for irrigation at the vegetable farms in Korle-bu Teaching Hospital, Accra Metropolis, Ghana.

    Science.gov (United States)

    Pesewu, George A; Bentum, Daniel; Olu-Taiwo, Michael A; Glover, Kathreen K; Yirenya-Tawiah, Dzidzo R

    2017-01-01

    Many developing countries, including Ghana, are water stressed. As such, farmers, particularly those in urban areas, have adopted the use of wastewater for irrigation. This study evaluated the bacteriological water quality of the wastewater used for irrigation in the vegetable farms at Korle-Bu Teaching Hospital (KBTH), Accra Metropolis, Ghana. In all, 40 wastewater samples were collected and analysed bacteriologically using the total aerobic plate count method. The isolated bacteria were identified biochemically using Bergey's manual for determinative bacteriology. Mean total bacterial colony count values in the range of 2.75-4.44 × 10 5 CFU/100 mL were isolated which far exceeds values of 1 × 10 3 /100 mL recommended by the World Health Organization (WHO) for unrestricted irrigation of crops likely to be eaten raw. Enterobacter cloacae (51.4%), Klebsiella sp. (24.1%), Pseudomonas aeruginosa (11.3%), Salmonella typhi (10.6%), Escherichia coli (2.2%) and Proteus sp. (0.4%) were the predominant bacteria isolated. Growers should use treated wastewater for farming while processors and consumers should minimize contamination risks of produce from the vegetable farms/garden to the plate. © The Author(s) 2016.

  19. Evaluation of methods to prepare samples of leafy green vegetables for preenrichment with the Bacteriological Analytical Manual Salmonella culture method.

    Science.gov (United States)

    Jacobson, Andrew Patrick; Gill, Vikas Singh; Irvin, Kari Anne; Wang, Hua; Hammack, Thomas Seyliard

    2012-02-01

    Three sample preparation procedures, soak, stomach, and blend, were evaluated using the Bacteriological Analytical Manual Salmonella culture method with eight types of leafy green produce. In the soak method, test portions were added to lactose broth without homogenization; in the stomach method, test portions were stomached with lactose broth; and in the blend method, test portions were blended with lactose broth. Twenty artificially contaminated test portions were analyzed with each procedure in individual experimental trials. The number of test portions identified as positive were compared among the procedures. Statistically significant differences were identified with Fisher's exact two-tailed F test (P < 0.05). Where differences did occur (P < 0.05), the soak procedure was the most effective or was at least as effective as homogenization by either blending or stomaching. Statistically significant differences most frequently occurred with romaine lettuce and cabbage; for these items, blending was significantly less effective than the soak procedure. Overall, for all of the produce types examined, results showed that the soak procedure was more effective than either of the homogenization procedures in recovering Salmonella from leafy green produce. Of the 540 test portions examined by each sample preparation method, 344 were positive for the presence of Salmonella by soaking, 293 by stomaching, and 232 by blending. We recommend that the soak procedure replace homogenization for the analysis of leafy green produce because the soak procedure is more productive than homogenization by either blending or stomaching of the leafy green produce types as reported herein.

  20. Molecular and bacteriological investigation of subclinical mastitis caused by Staphylococcus aureus and Streptococcus agalactiae in domestic bovids from Ismailia, Egypt.

    Science.gov (United States)

    Elhaig, Mahmoud Mohey; Selim, Abdelfattah

    2015-02-01

    A study was carried out to establish the prevalence of subclinical mastitis (SCM) in smallholder dairy farms in Ismailia, Egypt. A total of 340 milking cows and buffaloes were sampled from 60 farms, and 50 nasal swabs were collected from consenting farm workers. Milk samples were subjected to California mastitis test (CMT) and the positive samples were examined by bacterial culture and PCR to identify etiological agents. Based on CMT, the prevalence of SCM was 71.6 % in cattle and 43.5 % in buffaloes while the prevalence was 25.2 % at cow-quarter level and 21.7 % at buffaloes-quarter level. Bacteriological analysis showed that the most frequently identified bacteria were Staphylococcus (S.) aureus (38.3 %) and Streptococcus (Str.) agalactiae (20 %). The diagnostic sensitivity of PCR compared to bacterial culture was superior with S. aureus and Str. agalactiae detection being 41 and 22.6 %, respectively. Furthermore, methicillin-resistant S. aureus (MRSA) strains occurred in 52.2 and 45 % of isolates of animals and workers, respectively. Subclinical mastitis due to S. aureus and Str. agalactiae is endemic in smallholder dairy herds in Ismailia. The occurrence of MRSA in animals and workers highlights a need for wide epidemiological studies of MRSA and adopting control strategies.

  1. [Clinical and bacteriological profiles of the urinary infections associated the VIH/AIDS in hospital area of Bamako, Mali].

    Science.gov (United States)

    Dao, S; Oumar, A A; Dembele, J P; Noutache, J L; Fongoro, S; Maiga, I; Bougoudogo, F

    2007-01-01

    The syndrome of immunodepression is the bed of multiple infections of which urinary infections. The goal of this study was to determine the aspects clinical and bacteriological urinary infections during the AIDS with the service of the infectious diseases of the hospital of the Point G of February 1, 2003 to June 30 2005. The diagnosis of the urinary infection was retained on the basis of bacteria number > or =10(4) bacteriury and or leucocytes count > or =10(5)/mm3. The prevalence of the urinary infection was estimated at 8.85%. The principal clinical aspects were a symptomatic pyelonephritis 73.5%, the leucocytiury 11.8%, the cystitis 8.8%, and acute prostatitis 5.9%. Escherichia coli was the most frequent bacterium (46.7%). The sensitivity of the germs was 91.7% with the aminosides, 90.9% with the fluoroquinolones, from 63.6 to 80% respectively with the cephalosporines of first and second generation. Resistance to ampicilline, chloramphenicol and sulfamides was about 72 and 80%. The systematic research of the urinary infection is necessary during the AIDS and the antibiotherapy of choice in first intention in absence of etiologic possibility of diagnosis should be the aminosides and or the fluoroquinolones.

  2. Serological and bacteriological responses of water buffalo (Bubalus bubalis) vaccinated with two doses of Brucella abortus strain RB51 vaccine.

    Science.gov (United States)

    Ramnanan, Anil; Diptee, Michael; Asgarali, Zinora; Campbell, Mervyn; Adesiyun, Abiodun Adewale

    2012-10-01

    Thirty-two water buffalo (Bubalus bubalis) calves aged 6–10 months were used to evaluate serological responses to Brucella abortus strain RB51 (RB51) vaccination in a dose-response study and to compare the use of two selective media for the isolation of RB51. The animals were randomly divided into three treatment groups. Groups I-III received the recommended vaccine dose (RD) twice 4 weeks apart, RD twice 18 weeks apart and saline once, respectively. Lymph nodes were excised from the three groups and subjected to bacteriological examination to determine the frequency of detection of RB51. Pre- and post-vaccination blood samples were collected and tested for B. abortus antibodies using the buffered plate agglutination test (BPAT), complement fixation test (CFT), and dot-blot assay. Sera taken at all post-inoculation weeks (PIW) were negative for field strain B. abortus using the BPAT. Antibody responses to RB51 were demonstrated in all vaccinates but not in controls by CFT and dot-blot assay from 1 PIW up to 16 weeks following booster vaccination. The agreement for both assays was 80.7% and there was a linear interdependence with a Pearson's correlation coefficient value of 0.578. The frequency of isolation of RB51 from the two selective media used was not significantly different (P > 0.05).

  3. Bacteriological And Nutritional Analysis Of Groundnut Cake Sold In An Open Market In Samaru Zaria-Kaduna State

    Directory of Open Access Journals (Sweden)

    Oko

    2015-05-01

    Full Text Available Abstract Bacteriological and nutritional analysis of groundnut cake powder sold in open market at Samaru-Zaria was studied. The samples collected from four zones of the study area were analysed for possible microbiological contamination and its nutritional quality. The results indicated a microbial load of 1.93 x 105 cfug and 1.94 x 105 cfug for zones A and B respectively 1.01 x 105 cfug for zone C and 2.37 x 105 cfug for zone D. The bacterial isolates found to be associated with the groundnut cake powder in this study included Klebsiella oxytoca Staphylococcus aureus Bacillus cereus E. coli P. aeruginosa and Streptococcus feacalis. The nutrients content of the sample included carbohydrates 55.15 moisture 12.65 lipid 15.40 protein 12.60 ash 3.95 and crude fibre 0.25. Groundnut cake sold in the study area is highly contaminated with bacteria except for samples from zone C which is within the Food and Drugs Agency FDA recommendation of 1.0 x 105cfuml as allowable microbial contamination for food. The high level of microbial contamination is a serious cause for concern as it may trigger epidemics. However the product is highly nutritious.

  4. Bacteriological Study of 100 Cases of Pyodermas with Special Reference to Staphylococci, Their Antibiotic Sensitivity and Phage Pattern

    Directory of Open Access Journals (Sweden)

    T V Ramani

    1980-01-01

    Full Text Available One hundred new cases of pyodenna attending King George Hospital, Vishakhapatnam were investigated bacteriologically with antibiotic sensitivity of all the strains isolated and phage typing of coagulm positive staphylocci. Among these 50 had impetigo and 15 each ahd furunculosis and fouiculitis. The remaining included various other clinical entities. Children under 10 years were observed to have high incidence of pyoderma. A total of 88 strains of staphylococci (77 coagulase positive and 11 coagulase negative strains 25 strains of beta haemolytic streptococci and 3 strains of Klebsiella were isolated. Staphylococci were found to be the commonest aetiological agents either single or in association with other organisms. Of the 76 strains of coagulase positive staphylococci 32 strains were not phage typable and among the 44 typable strains 17 (38% belonged to group III and 15 (36.5% to mixed group. Coagulase positive staphylococci showed high sensitivity to garamycin, kanamycin and erythromycin and high resistance to penicillin and streptomycin. Multiple drug resistance was also high among these strains. Coagulase negative staphylococci were found to be more sensitive with less incidence of multiple drug resistance. Most of multiple drug resistant strains belonged to group III phage types. Beta haemolytic streptococci were found to be highly sensitive to all the antibiotics tested.

  5. COMPARISON THE NUMBER OF BACTERIA BETWEEN WASHING HANDS USING SOAP AND HAND SANITIZER AS A BACTERIOLOGY LEARNING RESOURCE FOR STUDENTS

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    Satya Darmayani

    2017-11-01

    Full Text Available Hands are the principal carriers of bacterial diseases, therefore very important to know that washing hands with soap or hand sanitizer is highly effective healthy behaviors to reduce bacteria in the palm. This study aimed to determine the total number of bacteria between washing hands with soap and hand sanitizer, also applying the results of these studies as a learning resource in bacteriology. The research design was the true experiment with pretest-posttest control group research design and laboratory examination. Analysis of data using paired t-test and independent sample t-test with α = 0.05. The result using paired t-test obtained t count= 2.48921> t 0.05 (14 = 2.14479 (with liquid soap, obtained t count= 2.32937> t 0.05 (14 = 2.14479 (with hand sanitizer. As for the comparison of the total number of bacteria include washing hands with soap and hand sanitizer using independent samples t-test obtained results there were differences in the total number of bacteria include washing hands with liquid soap and hand sanitizer with t count= 2.23755> t 0.05 ( 13 = 2.16037. That results showed hand sanitizer more effective to reduce the number of bacteria than the liquid soap, that was hand sanitizer 96% and liquid soap by 95%.

  6. The prevalence and bacteriology of asymptomatic bacteriuria among antenatal patients in Nnamdi Azikiwe University Teaching Hospital Nnewi; South Eastern Nigeria.

    Science.gov (United States)

    Oli, A N; Okafor, C I; Ibezim, E C; Akujiobi, C N; Onwunzo, M C

    2010-12-01

    Urinary tract infection in pregnancy leads to poor pregnancy outcome. Diagnosis and treatment of asymptomatic bacteriuria markedly improves pregnancy outcome as well as reduce the incidence of acute pyelonephritis. To determine the prevalence and bacteriology of asymptomatic bacteriuria among Antenatal patients in our centre, and to know if routine screening will be justifiable. This was a prospective study carried out between April and August 2008. Sample size was statistically determined. Women who consented were interviewed and mid stream urine samples were collected and processed in the microbiology laboratory, using standard microbiological methods. Out of 357 women studied, 65(18.21%) had significant bacteriuria. Escherichia coli was the commonest isolate (25.6%), while proteus mirabilis was the least frequent isolate (3.66%). Women in third trimester had the highest prevalence (25.68%) while those in the first trimester had the least (15.79%). Women that had only primary education had the highest prevalence (27.50%) while those that had tertiary education had the least prevalence (21.10%). The prevalence of significant asymptomatic bacteriuria among the women studied was high. Screening of all the pregnant women and treatment will reduce the incidence and complications of overt urinary tract infection in pregnancy among these women.

  7. [Bacteriological study of oral cavity of people of Mexican origin to determine etiology agents of human infections in hand bite].

    Science.gov (United States)

    Cañedo-Guzmán, Cristhyan Baruch; Espinosa-Gutiérrez, Alejandro; Guzmán-Murillo, María Antonia

    2013-01-01

    Hand infections secondary to human bites often leave serious consequences on the functioning of the hand. Such infections are caused by different bacteria. Most bacteriological studies have been made to people of Anglo-Saxon origin or descent, and based on these findings; provide treatment to patients of different origins which may not always be as effective. Descriptive, internal stratified 17 patients were isolated samples of oral cavity and dental plaque bacterial species to identify and define the possible treatment according to the species identified. Microorganisms were isolated Gram (+) and Gram (-) belonging to the normal flora of the oral cavity and dental plaque in all the cases studied, presenting a variable number of microorganisms according to age but not by sex. The group of Gram-positive bacteria isolated showed sensitivity to: erythromycin, chloramphenicol and ciprofloxacin. In the group of Gram negative: kanamycin, amoxicillin + clavulanic acid, ciprofloxacin and norfloxacin, E. Corrodens sensitive to the group of quinolones as ciprofloxacin, norfloxacin as well as ceftriaxone and cefoperazone sulbactam. The bacterial species that are commonly found in normal flora of the oral cavity and dental plaque may be potential pathogens in a hand injury where to find the appropriate conditions for their development.

  8. Implementation of Geographical Information System for Bacteriological Contamination Analysis on Refill Drinking Water Depot (Study in Tembalang District)

    Science.gov (United States)

    Rahmitha, Amelia; Utami, Endang Sri; Sitohang, Marya Yenita

    2018-02-01

    People used refilled-drinking-water for household and food stall because its efficient and low cost. Based on Indonesian Health Ministry regulation, it should not have any coliform bacteria. This study aimed to describe the bacteriological contamination of refilled drinking water using geographical information system (GIS). In this research, it was used an analytic observational method. The samples were from all available (37) depots in Tembalang district, one form each depot took used a sterile bottle. Contamination of bacteria was identified by Most Probable Number (MPN) method lactose broth media, Mac Conkey media, and IMVIC media. The depot samples were then plotted on (GIS). This study showed 95% samples were not feasible to consume since they contamined coliform. All sub-district had one that contaminated by coliform, 75% sub-districts had depots that contaminated Escherichia coli, while 55% sub-districts had depots that contaminated with other bacteria. The internal risk factors of the contamination were the absence of hygiene-sanitation worthy certificate (95%), depots location near to pollution sources (5%), and the misused of UV light. The external risk factor was lack of quality control that was not as the sterilization from office health Semarang city. Policy reinforcement should be done to all of the depots.

  9. [Bergey's Manual of Systematic Bacteriology (second edition) Volume 5 and the study of Actinomycetes systematic in China].

    Science.gov (United States)

    Ruan, Jisheng

    2013-06-04

    Bergey's Manual of Systematic Bacteriology (hereinafter referred to as "Bergey's Manual") is the collection of academic views accepted by taxonomists in many countries. It has scientificity, unitarity and practicality. "Bergey's Manual" (special issue of Actinomycetes) divided into two parts (part A and part B) was published in May, 2012. Under the guidance and the organization of Michael Goodfellow et al., the great work has been completed successfully in May 2012. "Bergey's Manual" made a great modification on the systematic of Actinomycetes and formally set up the phylum of Actinobacteria, which encompasses 6 classes, 23 orders (include one order incertae sides), 53 families, 222 genera and about 3000 species. The taxonomic catalogue is Bacteria, phylum of Actinobacteria, under the phylum there are class, order, family, genera and species. "Bergey's Manual" collected a great deal of new taxa, which were published in IJSEM (International Journal of Systematic and Evolutionary Microbiology) by Chinese scientists. We need to indicate that due to its too rigorous, conservative writing purpose and long publication periods, "Bergey's Manual" fails to collect new research results using the molecular approaches of multilocus sequence analysis "MLSA", gene chip technology and genome technologies, which however will profoundly change the taxonomy of prokaryotes in the near future.

  10. [Consensus for antimicrobial susceptibility testing for Enterobacteriaceae. Subcommittee on Antimicrobials, SADEBAC (Argentinian Society of Clinical Bacteriology), Argentinian Association of Microbiology].

    Science.gov (United States)

    Famiglietti, A; Quinteros, M; Vázquez, M; Marín, M; Nicola, F; Radice, M; Galas, M; Pasterán, F; Bantar, C; Casellas, J M; Kovensky Pupko, J; Couto, E; Goldberg, M; Lopardo, H; Gutkind, G; Soloaga, R

    2005-01-01

    Taking into account previous recommendations from the National Committee for Clinical Laboratory Standards (NCCLS), the Antimicrobial Committee, Sociedad Argentina de Bacteriología Clínica (SADEBAC), Asociación Argentina de Microbiología (AAM), and the experience from its members and some invited microbiologists, a consensus was obtained for antimicrobial susceptibility testing and interpretation in most frequent enterobacterial species isolated from clinical samples in our region. This document describes the natural antimicrobial resistance of some Enterobacteriaceae family members, including the resistance profiles due to their own chromosomal encoded beta-lactamases. A list of the antimicrobial agents that should be tested, their position on the agar plates, in order to detect the most frequent antimicrobial resistance mechanisms, and considerations on which antimicrobial agents should be reported regarding to the infection site and patient characteristics are included. Also, a description on appropriate phenotypic screening and confirmatory test for detection of prevalent extended spectrum beta-lactamases in our region are presented. Finally, a summary on frequent antimicrobial susceptibility profiles and their probably associated resistance mechanisms, and some infrequent antimicrobial resistance profiles that deserve confirmation are outlined.

  11. The dogma of bacteriology and other events as spearheads of virology Dogma de la bacteriología y otros eventos como puntas de lanza de la virología

    Directory of Open Access Journals (Sweden)

    Charles Volcy

    2009-11-01

    Full Text Available

    This review analyzes some of the main events during the twentieth century that led to the birth of virology. In the literature of the time, a relationship of causality was established between virus and disease, and within this pathological context, the notions of fixed virus, volatile virus, contagium vivum fluidum and contagium fixum were formed. The transformation of the virus concept as a technoscientific object took place based on the criterion of filterability and on the methodology devised for the bacteriology dogma. Studies on tobacco mosaic virus are highlighted, as well as those on viral diseases of human beings and animals, which led to the discovery of these submicroscopic agents through the appropriation of technologies and the impulse triggered by political and economic factors.

    Esta investigación analiza algunos de los eventos principales del siglo XIX que condujeron hacia la cristalización de la virología. En los documentos de esta investigación analiza algunos de los eventos principales del siglo XIX que condujeron hacia la cristalización de la virología. En los documentos de aquella época, se plasmó una relación de causalidad entre virus y enfermedad o contagio, y en este contexto patológico, se elaboraron las nociones de virus fijo, virus volátil, contagium vivum fluidum y contagium fixum. De otro lado, la transformación conceptual hacia el virus como objeto tecnocientífico tuvo lugar con base en el criterio de filtrabilidad y en la metodología trazada por la bacteriología convertida en dogma. Asimismo, se

  12. Spatial variation of physicochemical and bacteriological parameters elucidation with GIS in Rangat Bay, Middle Andaman, India

    Science.gov (United States)

    Dheenan, P. S.; Jha, Dilip Kumar; Vinithkumar, N. V.; Ponmalar, A. Angelin; Venkateshwaran, P.; Kirubagaran, R.

    2014-01-01

    The purpose of this study was to determine the concentration, distribution of bacteria and physicochemical property of surface seawater in Rangat Bay, Middle Andaman, Andaman Islands (India). The bay experiences tidal variations. Perhaps physicochemical properties of seawater in Rangat Bay were found not to vary significantly. The concentration of faecal streptococci was high (2.2 × 103 CFU/100 mL) at creek and harbour area, whereas total coliforms were high (7.0 × 102 CFU/100 mL) at mangrove area. Similarly, total heterotrophic bacterial concentration was high (5.92 × 104 CFU/100 mL) in mangrove and harbour area. The Vibrio cholerae and Vibrio parahaemolyticus concentration was high (4.2 × 104 CFU/100 mL and 9 × 103 CFU/100 mL) at open sea. Cluster analysis showed grouping of stations in different tidal periods. The spatial maps clearly depicted the bacterial concentration pattern in the bay. The combined approach of multivariate analysis and spatial mapping techniques was proved to be useful in the current study.

  13. Experimental techniques; Techniques experimentales

    Energy Technology Data Exchange (ETDEWEB)

    Roussel-Chomaz, P. [GANIL CNRS/IN2P3, CEA/DSM, 14 - Caen (France)

    2007-07-01

    This lecture presents the experimental techniques, developed in the last 10 or 15 years, in order to perform a new class of experiments with exotic nuclei, where the reactions induced by these nuclei allow to get information on their structure. A brief review of the secondary beams production methods will be given, with some examples of facilities in operation or under project. The important developments performed recently on cryogenic targets will be presented. The different detection systems will be reviewed, both the beam detectors before the targets, and the many kind of detectors necessary to detect all outgoing particles after the reaction: magnetic spectrometer for the heavy fragment, detection systems for the target recoil nucleus, {gamma} detectors. Finally, several typical examples of experiments will be detailed, in order to illustrate the use of each detector either alone, or in coincidence with others. (author)

  14. Determination of the physico-chemical and bacteriological quality of water from hand dug wells in the Ga-West Municipality, Accra, Ghana

    International Nuclear Information System (INIS)

    Fatawu, B.

    2015-01-01

    The Ga-West Municipality is noted for high reported cases of Buruli Ulcer and other water-borne diseases. A large section of the population of the municipality depends on boreholes and hand-dug wells to meet their basic daily water requirement. The hand-dug wells and boreholes, unlike treated piped water and Water Kiosk Programmes in the communities of the study area are not frequently monitored for their bacteriological, physic-chemical parameters and trace elements content; to ascertain their suitability for drinking and other important domestic chores. The study assessed the quality (bacteriological, physico-chemical and trace elements) of water from hand-dug wells and boreholes in communities within the Ga West Municipality. Groundwater samples were collected in November and December 2014 from hand-dug wells and boreholes in twenty-three (19) communities within the Ga-West Municipality for the study. pH, Total Dissolved Solids, Electric Conductivity and Temperature were determined onsite with the portable Multifunctional HACH-Conductivity meter. HCO 3 - Alkalinity, Calcium and Total Hardness were determined by Titrimetry. Trace metals (Mn, Fe, Co, Ni, Cu, Pb, Cd, Zn) and Mg were determined by Atomic Absorption Spectrometry. For bacterial indicators, Aerobic Colony Count Technique was employed. Fecal Coliform and E-coli were determined using Violet Red Bile-salt Agar Media, and Indole Test used to confirm Ecoli. Plate Count Agar Media was used in the determination of Total Coliform. pH was acidic to weakly alkaline (4.5 - 8.5) for hand-dug wells and weakly acidic to near-neutral (6.4 - 7.5) in the boreholes. Total Dissolved Solids were generally high with about 35.7 % of the samples exceeding WHO recommended Guideline of 1000 mg/L in both hand-dug wells and boreholes. According to TDS classification by Freeze and Cherry, 74.3 % of the groundwater samples were classified as fresh, (TDS ‹100 mg/L) and 35.7 % as brackish (TDS › 100 mg/L). Total Hardness values

  15. Bacteriological Spectrum of Post Operative Orthopedic Implant Infections and Their Antibiogram

    Directory of Open Access Journals (Sweden)

    Satya Chandrika V

    2016-01-01

    Full Text Available Background: Infection is a major problem in orthopedics leading to implant failure and in severe cases, amputation and even mortality. Aim and Objectives: The aim of this study is to isolate and identify organisms from postoperative Orthopaedic implant infections and determine their antibiogram. Method and Materials: This prospective study was conducted on 50 cases of infected implants from Orthopaedics ward, Government General Hospital, st st Kakinda from 1 June 2014 to 1 November 2014 after obtaining clearance from Institutional Ethics Committee. Pus samples were collected using two sterile swabs. One is used for Gram's stain and the other for inoculation on MacConkey and Blood agar. Isolates were identied according to the standard protocols and antibiotic sensitivity was done by Kirby Bauer's disc diffusion technique. Results: Out of 50 samples, 45(90% were culture positive and 5(10% were sterile for aerobic bacteria. Staphylococcus aureus (30% is common isolate followed by Coagulase Negative Staphylococci (CONS (20%, Escherichia coli (16%, Klebsiella sps (10% , Pseudomonas aeruginosa (6%, Acinetobacter species (4%, Proteus species (4%. Most of Gram positive cocci are resistant to Methicillin (64% followed by Ciprooxin (36% and Cotrimoxazole (28%. They were mostly sensitive to Vancomycin, Linezolid, Amoxycillin Clavulanic acid and Cefotaxime. Gram negative bacilli are resistant to Cotrimoxazole (65% followed by Ciprooxacin (60% and Ceftriaxone (60% and sensitive to Carbapenems (80%, Piperacillin Tazobactam (60% and Amoxycillin Clavulanic acid (60%. Extended Spectrum Beta Lactamase (ESBL production is seen in 60% of Gram negative bacilli. Conclusions: The incidence of multidrug resistance pathogens as a cause of implant infections is rising. Adequate preventive measures should be enforced to prevent the spread of antibiotic resistant organisms

  16. Bacteriological quality of drinking water in the Atebubu-Amantin District of the Brong-Ahafo Region of Ghana

    Science.gov (United States)

    Tekpor, M.; Akrong, M. O.; Asmah, M. H.; Banu, R. A.; Ansa, E. D. O.

    2017-09-01

    The study was carried out to determine the bacteriological safety of water in hand-dug wells in the Atebubu-Amantin District of the Brong-Ahafo Region in Ghana. A total of 60 samples were collected from ten hand dug wells and analysed for total coliform (TC), faecal coliform (FC), E. coli (EC), Salmonella spp. (SP) and Enterococcus spp. (ES). Data was collected in both the rainy and the dry seasons. The results obtained showed that water from all the wells in the study area did not meet the World Health Organisation guideline and Ghana standard for drinking water of zero (0) coliform forming unit (cfu) per 100 ml for TC, FC, EC, SP and ES, respectively. Contamination was found to be high in the wells during the wet season as compared to the dry season. Wells (A1 to A5) which were close to septic tanks had high bacteria counts in both seasons. The total coliform counts ranged from 2.98 to 5.93 log cfu/100 ml in the wet season and 3.10-5.03 log cfu/100 ml in the dry season. There was drastic reduction of faecal coliform count from a range of 2.78-4.55 log cfu/100 ml in the wet season to 1.70-3.51 log cfu/100 ml in the dry season. The high bacteria count in wells A1 to A5 could be attributed to the closeness of the wells to the septic tank, and contaminant transport through the saturated underground zones. It is recommended that the water should be treated properly before drinking.

  17. Bacteriology of deep carious lesions underneath amalgam restorations with different pulp-capping materials - an in vivo analysis

    Directory of Open Access Journals (Sweden)

    Prasanna Neelakantan

    2012-04-01

    Full Text Available Microorganisms remaining in dentin following cavity preparation may induce pulp damage, requiring the use of pulp-capping agents with antimicrobial activity underneath permanent restorations. OBJECTIVE: The aims of this study were to analyze the bacteriological status of carious dentin and to assess the efficacy of different base underneath silver amalgam restorations. MATERIAL AND METHODS: This study was conducted on 50 patients aged 13 to 30 years. Sterile swabs were used to take samples after cavity preparation, which was assessed by microbiological culture to identify the microorganisms present. Following this, cavities were restored with silver amalgam, using one of the materials being investigated, as the base: calcium hydroxide (Group II, polyantibiotic paste (Group III, a novel light-cured fluoride-releasing hydroxyapatite-based liner (Group IV and mineral trioxide aggregate - MTA (Group V. In Group I, the cavities were restored with silver amalgam, without any base. After 3 months, the amalgam was removed and samples taken again and analyzed for the microbial flora. RESULTS: Lactobacilli were the most commonly isolated microorganisms in the samples of carious dentin. Groups IV and V showed negative culture in the 3-month samples. There was no statistically significant difference between Groups I, II and III. There was no significant difference between Groups IV and V (p>0.05. Both Groups IV and V showed significantly better results when compared to Groups I, II and III (p<0.05. CONCLUSIONS: The hydroxyapatite-based liner and MTA performed significantly better in terms of antibacterial activity than the other materials.

  18. Risk factors for bacteriological quality of bulk tank milk in Prince Edward Island dairy herds. Part 1: overall risk factors.

    Science.gov (United States)

    Elmoslemany, A M; Keefe, G P; Dohoo, I R; Jayarao, B M

    2009-06-01

    The objective of this study was to determine on-farm risk factors for bacteriological quality of bulk tank milk. Bulk tank raw milk quality was evaluated on all Prince Edward Island dairy herds (n = 235) over a 2-yr period (March 2005 to March 2007). Biweekly total bacterial, preliminary incubation, laboratory pasteurization, and coliform counts were conducted using a Petrifilm culture system. For the assessment of risk factors, a case-control study was conducted from January 2006 to May 2007. Case and control herds were defined based on the last 6 analyses of bulk tank bacterial counts before on-farm evaluation. Cases were herds that had multiple elevated counts for any of the parameters measured. A total of 69 herds (39 cases and 30 control herds) were evaluated. Data collection included 1) observation and questionnaire on basic hygiene and farm management practices; 2) complete wash analysis of the milking equipment, monitoring the presence of bacterial films on equipment and evaluation of cooling system function; and 3) environmental and cow hygiene scoring. Data were analyzed using multivariable logistic regression. The results of the final model indicated that high alkalinity in the wash water and poor teat-end cleanliness were associated with high bacterial counts in bulk tank milk (odds ratios = 12 and 5.3, respectively). It was also observed that high water temperature of detergent wash and the use of a water softener were associated with low bacterial counts in bulk tank milk (odds ratios = 0.87 and 0.11, respectively). A significant association between udder hair clipping and teat-end cleanliness was also observed. In conclusion, this study highlights the importance of udder hygiene and milking system washing factors on hygienic quality of bulk tank milk.

  19. Physico-chemical and bacteriological quality of water from shallow wells in two rural communities in Benue state, Nigeria

    International Nuclear Information System (INIS)

    Terngu, A.J.; Hyacinth, O.A.; Rufus, S.A.

    2010-01-01

    Ground water abstraction from shallow wells is widely practiced in the Obi and in Oju rural areas of Benue State, Central Nigeria, as a means of fighting guinea worm infestation associated with the surface water sources (streams) in these areas. To ascertain the physico-chemical and bacteriological quality of the water used by the population, water samples from 27 shallow wells in Obi and 19 Oju were taken and examined for key health -related quality parameters using routine methods. In Obi, the ground water colour ranged from 4.0-80.0 TCU, conductivity 55.2- 1600.0 ILS/cm, pH 6.1-8.6, TDS 38.6-1286 mg/L, turbidity 1.0 - 55.0 NTU, arsenic 0.001- 0.210 mg/L, copper 0.01-2.53 mg/L, fluoride 0.08-1.82 mg/L and nitrate 10.8-63.0 mg/L; while in Oju, colour varied from 2.0-87.0 TCU, conductivity 1 07.4-1375 LS/cmp, H 6.4-8.53, TDS 75.2- 1150 mg/L, turbidity 3.0-48.0 NTU, arsenic 0.001-0.023 mg/L, copper 0.01-2.10 mg/L, fluoride 0.01-1.54 mg/L and nitrate 10.2-59.7 mg/L. Some of these values in some instances exceed the WHO standard for drinking water. Alongside with the presence significant total coliform count in most of the wells (0-47/100 mL in Oju and 0-53/100 mL in Obi), the available water is considered largely unsafe for human consumption as obtained. (author)

  20. BACTERIOLOGICAL PROFILE AND ANTIBIOTIC SENSITIVITY PATTERN IN ACUTE EXACERBATION OF ADVANCED CASES OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE (COPD

    Directory of Open Access Journals (Sweden)

    Avik

    2016-01-01

    Full Text Available Acute exacerbations are significant and frequent events in the natural history of chronic obstructive pulmonary disease. Majority of these exacerbations are of infectious aetiology, bacteria being responsible for 30-50% of these cases. With not many studies of similar type being conducted in the Indian context, this study was undertaken with the purpose of determining the bacteriology of acute exacerbations of chronic obstructive pulmonary disease in hospitalized patients with advanced disease and their antibiotic susceptibility pattern to formulate a cost effective algorithm for antibiotic usage while at the same time reducing the chances of emergence of drug resistance. Sputum sample from a total of 338 patients were send for Gram’s stain and culture sensitivity testing using an array of the commonly used antibiotics. Pathogenic bacteria were isolated from 203 (60.1% samples. Gram negative bacteria were isolated from 79.8 percent (162/203 cases while the rest were Gram positive. Klebsiella species were the commonest (49.2%; 100/203 Gram negative isolates from the sputum samples. Among the gram negative organisms, Carbapenem had the highest sensitivity (90.2% followed by Amikacin, Ciprofloxacin and Piperacillin-Tazobactam. Linezolid was found to be 100 percent sensitive amongst the Gram positive organisms while both Amoxicillin Clavulanate and Azithromycin showed a rather low sensitivity profile overall. 5.0 percent of the Klebsiella infections were multi drug resistant. It was thereby concluded that either Amikacin, Ciprofloxacin or Piperacillin-Tazobactam for be considered for Gram negative organisms and Linezolid be considered for Gram positive organisms as first line antibiotics in empirical therapy while Carbapenems may be kept as reserve drugs should the first line drugs fail.

  1. Bacteriological profile and antibiotic sensitivity pattern of neonatal septicaemia in a rural tertiary care hospital in North India

    Directory of Open Access Journals (Sweden)

    S Thakur

    2016-01-01

    Full Text Available Background: There is not much published literature on neonatal septicemia available for the Sub-Himalayan region of North India. Hence, we undertook this study to find out the bacteriological profile and antibiotic sensitivity pattern of neonatal septicemia in the neonatal Intensive Care Unit. Material and Methods: Blood cultures were performed for all clinically suspected neonatal septicemia cases for 1-year. Identification of all pathogenic isolates was followed by antibiotic sensitivity testing. Results: We did blood cultures for 450 neonates and 42% were culture positive. Early onset sepsis were 92 (49% and 96 (51% were late onset sepsis. Gram-positive isolates were 60% and 40% were Gram-negative. Staphylococcus aureus (40%, coagulase negative Staphylococcus species (16%, non-fermenter group of organisms (NFGOs (15%, and Klebsiella pneumoniae (10% were the main isolates. Nasal cannula 101 (54%, birth asphyxia 91 (48%, and prematurity 73 (38% were the prominent risk factors associated with septicemia. Gram-positive organisms were highly resistant to penicillin (87% whereas Gram-negative isolates showed high resistance to third generation cephalosporins (53–89% and aminoglycosides (50–67%. The S. aureus isolates were methicillin-resistant in 41% whereas extended spectrum beta lactamase production was seen in 48% Gram-negative isolates.Conclusion: Our study highlights the recent emergence of Gram-positive organisms as predominant cause of neonatal septicemia in this part of Sub-Himalayan region, along with the review of literature which shows similar results from North India and rest of the world too. Though Gram-negative bacteria still remain the main cause of mortality in neonatal septicemia, we want to dispel the common notion among practitioners that they are the predominant isolates in neonatal septicemia.

  2. Polymerase Chain Reaction, Bacteriologic Detection and Antibiogram of Bacteria Isolated from Otitis Media with Effusion in Children, Shiraz, Iran

    Directory of Open Access Journals (Sweden)

    Mahmood Shishegar

    2011-12-01

    Full Text Available Background: Otitis media with effusion is one of the leading causes of hearing loss in children. Effective treatment of effusion in the middle ear requires appropriate empirical treatment and characterization of responsible pathogens. Objective of the present study was to detect pathogens in clinical samples from patients with otitis media with effusion in our area and to determine the sensitivity profile of isolated organisms to commonly used antibiotics. Methods: Sixty three samples of middle ear effusion were aseptically obtained from 36 children, who had been treated up to at least two weeks before sampling. They were analyzed using standard bacteriological and multiplex polymerase chain reaction (PCR assays. Antibiotic susceptibility tests were also performed. Results: PCR analysis showed that DNA of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis were present in 60 (95.2% of the samples. The culture-positive effusion for Streptococcus Pneumoniae, HaemophilusInfluenzae and Moraxella catarrhalis was 34.9%. Almost all isolates of Streptococcus pneumoniaee were sensitive to ciprofloxacin and erythromycin, and none of them was sensitive to co-trimoxazole. None of H. Influenzae isolates was sensitive to erythromycin, cefixim, co-trimoxazole, ampicillin and amoxicillin. None of M. Catarrhalis isolates was sensitive to ceftriaxone, co-trimoxazole, ampicillin and amoxicillin. Conclusion: Compared with other studies using PCR method, the number of H. influenza isolates was in higher in the present study (95.2%. Antibiotic sensitivity profiles of pathogens isolated in this study were different from others. Thus, we can determine empirical antibiotic therapy based on sensi-tivity profile in our geographic area.

  3. New techniques for growing anaerobic bacteria: experiments with Clostridium butyricum and Clostridium acetobutylicum

    International Nuclear Information System (INIS)

    Adler, H.I.; Crow, W.D.; Hadden, C.T.; Hall, J.; Machanoff, R.

    1983-01-01

    Stable membrane fragments derived from Escherichia coli produce and maintain strict anaerobic conditions when added to liquid or solid bacteriological media. Techniques for growing Clostridium butyricum and Clostridium acetobutylicum in membrane-containing media are described. Liquid cultures initiated by very small inocula can be grown in direct contact with air. In solid media, colonies develop rapidly from individual cells even without incubation in anaerobic jars or similar devices. Observations on growth rates, spontaneous mutations, radiation, and oxygen sensitivity of anaerobic bacteria have been made using these new techniques

  4. A STUDY TO FIND OUT THE BACTERIOLOGY OF TONSILLAR SURFACE AND CORE, AMONG PATIENTS UNDERGOING TONSILLECTOMY AT A TERTIARY CARE HOSPITAL IN SOUTH INDIA

    Directory of Open Access Journals (Sweden)

    Binu

    2016-05-01

    Full Text Available BACKGROUND The study was aimed at finding out the bacteriology of the tonsillar surface and core, in those patients undergoing tonsillectomy at a tertiary care hospital. The study was also planned to look for the antibiotic resistance pattern among organisms identified and to see whether a tonsillar surface swab can be used as a surrogate for finding out the aetiopathogenesis of tonsillar infections. METHODS This is a 2-year cross-sectional study of 100 patients undergoing who consented for the study, after taking a smear from the surface of the tonsil. Tonsillectomy was done by dissection and snare method. The core of the tonsil is then biopsied and microbiological culture and sensitivity is done. The bacteria were identified by morphology and staining characteristics and cultured using standard laboratory procedures. Antimicrobial susceptibility was done by Kirby-Bauer disc diffusion method. The data was digitalised using a data entry platform using Epidata and analysed using SPSS 16. Relativity between organism identified as the surface and core was tested using Chi-square tests and Kappa statistics. RESULTS There were significant differences between the organisms identified from the tonsillar surface and the core; out of the 200 specimens, only 48 showed a similar bacteriology. The viability between the surface and the core specimens were measured using Kappa statistics and was found to be 0.04, which shows a very poor agreement between them. Assessment of antibiotic sensitivity shows that Staph aureus was resistant to most of the commonly used antibiotics. H. influenza isolated showed 70% susceptibility to erythromycin and cotrimoxazole. While group A β haemolytic streptococci showed 95% susceptibility to ampicillin and 100% to erythromycin. CONCLUSION Staph. aureus, H. influenza, Streptococci pneumonia were the major pathogenesis identified from the tonsil of the patients undergoing tonsillectomy, and bacteriology was different between

  5. Latent class analysis of the diagnostic characteristics of PCR and conventional bacteriological culture in diagnosing intramammary infections caused by Staphylococcus aureus in dairy cows at dry off

    DEFF Research Database (Denmark)

    Cederlöf, Sara Ellinor; Toft, Nils; Aalbæk, Bent

    2012-01-01

    ABSTRACT: BACKGROUND: Staphylococcus aureus is one of the most common causes of intramammary infections in dairy cows at dry off. Reliable identification is important for disease management on herd level and for antimicrobial treatment of infected animals. Our objective was to evaluate the test...... characteristics of PathoProof TM Mastitis PCR Assay and bacteriological culture (BC) in diagnosing bovine intramammary infections caused by S. aureus at dry off at different PCR cycle threshold (Ct)-value cut-offs. METHODS: Sterile quarter samples and non-sterile composite samples from 140 animals in seven herds...

  6. Clinical and bacteriological characteristics of invasive pneumococcal disease after pneumococcal 10-valent conjugate vaccine implementation in Salvador, Brazil

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    Carolina Regis Leite

    2016-01-01

    Full Text Available Invasive pneumococcal disease is a relevant public health problem in Brazil, especially among children and the elderly. In July/2010 a 10-valent pneumococcal conjugate vaccine was introduced to the immunization schedule of Brazilian children under two years of age. Between July/2010 and December/2013 we conducted a case-series study on invasive pneumococcal disease in Salvador, Brazil to describe the clinical and bacteriological profile of invasive pneumococcal disease cases during the post-implementation period. Eighty-two cases were eligible. Mean age was 31 years (interquartile range, 3–42; 17.1% and 30.5% were under 2 years and 5 years, respectively. Pneumococcal meningitis (n = 64, 78.1%, bacteraemic pneumococcal pneumonia (n = 12, 14.6% and bacteraemia (n = 6, 7.3% were the clinical syndromes identified. Thirty-three different serotypes were found. Of these, serotype 14 (n = 12, 14.6% was the most common, followed by 23F (n = 10, 12.2%, 12F (n = 8, 9.8%, 18 C (n = 5, 6.1% and 6B (n = 5, 6.1%. Investigations conducted in Salvador in the pre-vaccine period did not identify serotype 12F as one of the most prevalent serotypes. Increase of serotype 12F was observed in different regions of Brazil, in the post-vaccine period. Among children under two years of age, the target group for 10-valent pneumococcal conjugate vaccine, 11 (78.6% of the 14 isolated strains of Streptococcus pneumoniae belonged to vaccine serotypes; at least 50% of these children were not vaccinated. The relatively recent implementation of 10-valent pneumococcal conjugate vaccine in Brazil reinforces the need to maintain an active surveillance of invasive pneumococcal disease cases, considering the possible increase of invasive pneumococcal disease cases related to non-vaccine serotypes and the changes on the clinical presentation of the disease.

  7. Physico-chemical and Bacteriological Quality of Water from Shallow Wells in Two Rural Communities in Benue State, Nigeria

    Directory of Open Access Journals (Sweden)

    Akaahan, Terngu J.

    2010-06-01

    Full Text Available Ground water abstraction from shallow wells is widely practiced in the Obi and in Oju rural areas of Benue State, Central Nigeria, as a means of fighting guinea worm infestation associated with the surface water sources (streams in these areas. To ascertain the physico-chemical and bacteriological quality of the water used by the population, water samples from 27 shallow wells in Obi and 19 Oju were taken and examined for key health-related quality parameters using routine methods. In Obi, the ground water colour ranged from 4.0-80.0 TCU, conductivity 55.2-1600.0 µS/cm, pH 6.1-8.6, TDS 38.6-1286 mg/L, turbidity 1.0-55.0 NTU, arsenic 0.001-0.210mg/L, copper 0.01-2.53mg/L, fluoride 0.08-1.82mg/L and nitrate 10.8-63.0mg/L, while in Oju, colour varied from 2.0-87.0 TCU, conductivity 107.4-1375 µS/cm, pH 6.4-8.53, TDS 75.2-1150 mg/L, turbidity 3.0-48.0 NTU, arsenic 0.001-0.023 mg/L, copper 0.01-2.10 mg/L, fluoride 0.01-1.54 mg/L and nitrate 10.2-59.7 mg/L. Some of these values in some instances exceed the WHO standard for drinking water. Alongside with the presence significant total coliform count in most of the wells (0-47/100 mL in Oju and 0-53/100 mL in Obi, the available water is considered largely unsafe for human consumption as obtained. It is concluded that, while ground water abstraction may be a safety measure against guinea worm infestation it, nevertheless presents other health challenges to the rural population in the area, as the quality of the ground water is generally low.

  8. The serologic response to Salmonella enteritidis and Salmonella typhimurium in experimentally infected chickens, followed by an indirect lipopolysaccharide enzyme-linked immunosorbent assay and bacteriologic examinations through a one-year period

    DEFF Research Database (Denmark)

    Skov, M.N.; Feld, Niels Christian; Carstensen, B.

    2002-01-01

    uninfected as controls. The groups were monitored bacteriologically by examination of cloacal swabs and organs and serologically by examination of serum and egg yolk by a lipopolysaccharide enzyme-linked immunosorbent assay throughout the period. Within the first week, 100% of birds in both infected groups...... at the onset of egg production. For both S. typhimurium and S. enteritidis, positive bacteriologic cultures were obtained by sampling from internal organs at the end of the experiment, more than 1 yr from the time of infection. At the age of 6-7 wk, 50% of the chickens in the two infected groups showed...... a measurable serologic response in serum samples. The response persisted throughout the study in both serum and egg yell, samples. The inclusion of serologic methods is a valuable additional tool in the detection of salmonella in poultry, but serology should be used in conjunction with bacteriologic methods...

  9. Methods in aquatic bacteriology

    National Research Council Canada - National Science Library

    Austin, B

    1988-01-01

    .... Within these sections detailed chapters consider sampling methods, determination of biomass, isolation methods, identification, the bacterial microflora of fish, invertebrates, plants and the deep...

  10. Bacteriological Study of Pyoderma

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    C S Bhaskaran

    1979-01-01

    Full Text Available Two hundred and twenty cases of pyoderma were investigated to study the bacterial aetiology. Folliculitis formed the largest-clinical group followed by infectious eczematoid dermatitis, secondary infection, impetigo, miscellaneous, furuncles and acne in descending order of frequency. A total of 172 micro-organisms were isolated from the infected specimens examined. A single infecting organism was isolated from 65.5% and more than one type of organism from 6.3% of cases. No organism was isolated from 62 (29.2% cases, Coagulase positive staphylococcus was isolated from 107 (48.6% beta haemolytic streptococcus from 18 (8.2% and both these organisms from 11 (5% cases. Coagulase positive staphylococcus (69.8% was the predominant species followed by beta haemolytic streptococcus (17.4%, coaplm negative staphylococcus (8.1% and alpha haemolytic streptococcus (4.7%. The antibiotic resistance pattern showed maximum resistance to ampicillin, penicillin and polymyxin. None of the strains was found to be resistant to cephaloridine, neomycin and kanamycin. Low percentage of strains were found to be resistant to streptomycin, tetracycline, nitrofurazone, erythromycin, chloramphenicol and gentamycin. Of the strains of Staph. aureusphage typed, 35 (43.7% were not typable, 15 (18.7% belonged tophage group 111, followed by phage group II, group I and mixed group. The most prevalent phage type in group III was 42E,in group II type 3 C and in the group I type 52A. Multiple antibiotic resistant strains of staph aureus were found mostly in phage groups III and I and not in group 11.

  11. Dismantling techniques

    International Nuclear Information System (INIS)

    Wiese, E.

    1998-01-01

    Most of the dismantling techniques used in a Decontamination and Dismantlement (D and D) project are taken from conventional demolition practices. Some modifications to the techniques are made to limit exposure to the workers or to lessen the spread of contamination to the work area. When working on a D and D project, it is best to keep the dismantling techniques and tools as simple as possible. The workers will be more efficient and safer using techniques that are familiar to them. Prior experience with the technique or use of mock-ups is the best way to keep workers safe and to keep the project on schedule

  12. BACTERIOLOGICAL STUDY OF COAGULASE-POSITIVE AND COAGULASE-NEGATIVE STAPHYLOCOCCI IN RELATION TO METHICILLIN SENSITIVITY TESTING

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    Padmanabham Yalangi

    2016-10-01

    Full Text Available BACKGROUND Staphylococcus aureus has long been recognised as an important pathogen in human disease. Staphylococci infection occurs regularly in hospitalised patients and has serious consequences despite antibiotic therapy. Shortly after introduction of methicillin after clinical use Methicillin-Resistant Staphylococcus Aureus (MRSA were identified in many countries and become one of the most common causes of nosocomial infections. The aim of the study is to know the methicillin sensitivity of both coagulase-negative and coagulase-positive staphylococci isolated from various samples. MATERIALS AND METHODS 100 strains of staphylococci both coagulase positive and coagulase negative were isolated in the Department of Microbiology from various clinical samples. They were confirmed by morphology, staining methods and by using standard bacteriological procedures and biochemical reactions. Antibiotic susceptibility testing was performed by Kirby Bauer disc diffusion test. RESULTS Predominant species from pus were S. epidermidis (42.42% and from sputum S. haemolyticus (31.81% from blood S. haemolyticus (53.33%. 53% of strains produced beta-lactamase. Majority 47.22% by S. epidermidis from pus followed by S. haemolyticus 23.33% from pus. Beta-lactamase production was least from throat swab (5.55%. Out of 32 coagulase-positive staphylococci tested to methicillin 15 (46.87% were found to be sensitive, 17 (53.13% were found to be resistant. Out of 68 coagulase-negative staphylococci tested, 13 (19.11% were found to sensitive and 55 (80.88% were found to be resistant. 72% of strains were sensitive to novobiocin and 28% resistant to novobiocin. 43% showed drug resistance to 2 drugs. 14% to 3 drugs and 5 drugs. 6% of staphylococci sensitive to all the 10 drugs. CONCLUSION MRSA is a type of bacteria that is resistant to a number of widely used antibiotics. This means MRSA infections can be more difficult to treat than other bacterial infections. In recent years

  13. Incorporation of galactomannans in the diet of newly weaned piglets: effect on bacteriological and some morphological characteristics of the small intestine.

    Science.gov (United States)

    Van Nevel, Christian J; Decuypere, Jaak A; Dierick, Noel A; Molly, Koen

    2005-04-01

    In search of substances replacing antibiotics as growth promoters for farm animals, non-digestible oligosaccharides (NDO) or non-starch polysaccharides (NSP) have been proposed as possible alternatives. In this context, the influence of galactomannans on bacteriological and morphological aspects of the gastrointestinal tract in weanling pigs was investigated. Four groups of five newly weaned piglets received one of the following diets: control feed (C), C supplemented with guar gum (1%), C supplemented with locust bean gum (1%) and C supplemented with 10% of carob tree seeds meal as source of locust bean gum. The animals were euthanized after 11-12 days and digesta were sampled in stomach, jejunum (proximal and distal) and caecum, while mucosal scrapings and ring shaped tissue samples were taken of proximal and distal jejunum. On these samples bacteriological, biochemical and morphological determinations were carried out. Total count of bacteria in digesta and mucosal scrapings was not influenced by the different diets, with the exception of the proximal jejunum where a small decrease (0.5 log10 CFU) was noted with the guar gum and carob tree seeds diet. The number of E. coli increased by feeding both gums and carob tree seeds. With the latter diet, higher counts of streptococci were observed. In agreement with the lower concentration of lactic acid in jejunal contents, guar gum decreased the number of lactobacilli. Locust bean gum decreased the molar proportion of acetate in caecal contents while butyrate and valerate were augmented. Feeding the carob tree seeds resulted in shorter villi and a lower villus height/crypt depth ratio in the jejunum mucosa, which was an indication for a faster renewal rate of the epithelium. Both locust bean gum feeds significantly lowered the mitotic index in the crypts of the small intestine. Only with the carob tree seeds diet, viscosity of jejunal contents was increased. In conclusion, the effects of the addition of 1% of pure

  14. Acquired Techniques

    DEFF Research Database (Denmark)

    Lunde Nielsen, Espen; Halse, Karianne

    2013-01-01

    Acquired Techniques - a Leap into the Archive, at Aarhus School of Architecture. In collaboration with Karianne Halse, James Martin and Mika K. Friis. Following the footsteps of past travelers this is a journey into tools and techniques of the architectural process. The workshop will focus upon...

  15. Bacteriological, histological and functional examination of rat kidneys during 5-days gentamycin therapy of experimental E. coli pyelonephritis as compared to 9-days therapy

    International Nuclear Information System (INIS)

    Hagemann, I.; Briedigkeit, H.; Strangfeld, D.; Ditscherlein, G.; Schuerer, M.; Pietsch, R.; Kruse, L.; Camu, A.; Blank, W.

    1985-01-01

    Short-term antibiotic treatment is recommended in infections of the lower urinary tract but its effectiveness is questioned in upper urinary tract infections. 5 and 9-days treatment of experimental E. coli 022 pyelonephritis after unilateral nephrectomy in 127 male Wistar rats were compared. 9 mg gentamycin per kg b.w. twice daily were applied. 131 I-hippurate excretion was not decreased during the 5 day treatment but was temporarily diminished during 9-days treatment. Histologically the severe acute pyelonephritis was decreased after 9-days treatment but not after 5 days of treatment. Bacteriologically almost all the kidneys were sterile after 9-days treatment but the majority of the kidneys showed the injected strain of E. coli after 5-days treatment. The results indicated that the shortened treatment was much less effective in acute experimental pyelonephritis. (author)

  16. Identification of Escherichia coli and Trueperella pyogenes isolated from the uterus of dairy cows using routine bacteriological testing and Fourier transform infrared spectroscopy.

    Science.gov (United States)

    Jaureguiberry, María; Madoz, Laura Vanina; Giuliodori, Mauricio Javier; Wagener, Karen; Prunner, Isabella; Grunert, Tom; Ehling-Schulz, Monika; Drillich, Marc; de la Sota, Rodolfo Luzbel

    2016-11-28

    Uterine disorders are common postpartum diseases in dairy cows. In practice, uterine treatment is often based on systemic or locally applied antimicrobials with no previous identification of pathogens. Accurate on-farm diagnostics are not available, and routine testing is time-consuming and cost intensive. An accurate method that could simplify the identification of uterine pathogenic bacteria and improve pathogen-specific treatments could be an important advance to practitioners. The objective of the present study was to evaluate whether a database built with uterine bacteria from European dairy cows could be used to identify bacteria from Argentinean cows by Fourier transformed infrared (FTIR) spectroscopy. Uterine samples from 64 multiparous dairy cows with different types of vaginal discharge (VD) were collected between 5 and 60 days postpartum, analyzed by routine bacteriological testing methods and then re-evaluated by FTIR spectroscopy (n = 27). FTIR spectroscopy identified Escherichia coli in 12 out of 14 samples and Trueperella pyogenes in 8 out of 10 samples. The agreement between the two methods was good with a Kappa coefficient of 0.73. In addition, the likelihood for bacterial growth of common uterine pathogens such as E. coli and T. pyogenes tended to increase with VD score. The odds for a positive result to E. coli or T. pyogenes was 1.88 times higher in cows with fetid VD than in herdmates with clear normal VD. We conclude that the presence of E. coli and T. pyogenes in uterine samples from Argentinean dairy cows can be detected with FTIR with the use of a database built with uterine bacteria from European dairy cows. Future studies are needed to determine if FTIR can be used as an alternative to routine bacteriological testing methods.

  17. Evaluation of serology, bacteriological isolation and polymerase chain reaction for the detection of pigs carrying Actinobacillus pleuropneumoniae in the upper respiratory tract after experimental infection.

    Science.gov (United States)

    Chiers, Koen; Donné, Eef; Van Overbeke, Ingrid; Ducatelle, Richard; Haesebrouck, Freddy

    2002-09-24

    Pigs, asymptomatically infected with Actinobacillus pleuropneumoniae in their upper respiratory tract, can transmit the infection. Detection of such animals is indispensable to prevent the intake of the disease in a herd. This study was conducted to evaluate bacteriology, polymerase chain reaction (PCR) and serology for the detection of subclinically infected pigs. Pigs were inoculated onto the tonsils with an A. pleuropneumoniae serotype 9 strain (n=12, group 1) or phosphate buffered saline solution (PBSS) (n=5, group 2). To prevent infection of the lungs, pigs of group 1 were treated three times with sodium ceftiofur as an aerosol. A third group (n=5) was inoculated intranasally with the same strain. All animals were euthanized 30 days post-inoculation (dpi). In pigs of group 1, clinical signs were not observed. A small lung lesion was found in only one pig and A. pleuropneumoniae was isolated from this lesion. The bacterium was not isolated from the lungs of animals that did not develop lung lesions. A. pleuropneumoniae was demonstrated in tonsils of 9/12 animals using bacteriological isolation, whereas it was demonstrated in mixed bacterial cultures from tonsils of all 12 animals by PCR. In non-infected animals (group 2), clinical signs were not observed and A. pleuropneumoniae was not demonstrated in any sample. All intranasally infected animals (group 3) developed disease signs and lung lesions. High antibody titers against ApxI, ApxII and heat-stable antigens were detected in animals that developed lung lesions. Antibody titers against these antigens were low or absent in all other pigs. It was concluded that pigs carrying A. pleuropneumoniae in the upper respiratory tract generally do not show measurable antibodies in serum. Therefore, sensitive methods for the detection of the etiological agent such as PCR are required to identify carrier animals, while serological methods are not suitable.

  18. O grupo pullorum-gallinarum em provas bacteriologicas comparativas The pullorum-gallinarum group in comparative bacteriological tests

    Directory of Open Access Journals (Sweden)

    Genesio Pacheco

    1936-01-01

    acção de substancias antidiastasicas. h A analyse sôrologica das amostras estudadas não permittiu differenciar os typos entre si.Thirty eight strains of microorganisms of the pullorum-gallinarum group, of European, American or Brazilian origin were investigated bacteriologically in order to ascertain as to the fixity of their properties and the possibility of transforming a strain in another. a Five types could be distinguished in our strains: 1 - gasogenic pullorum; 2 - non-gasogenic pullorum; 3 - intermedius; 4 - gasogenic gallinarum?; 5 - non-gasogenic gallinarum. The first, two and fifth types are well known and recognized by specialists; the fourth group has been recognized by Beck & Eber, in 1920; the third group was recognized by ourselves, in 1935. b The table found in this paper summarizes the most important characteristics of these five types. The changes produced on neutral red, H²S production, fermentation of glycerol, isodulcitol, xylose, dulcite, sorbitol and maltose, the activity upon Seignette salt, gas production and appearance of the colonies, are among the characteristics on which the distinction of these five types is founded. c The gasogenic gallinarum type, on account of its action on sorbitol and xylose, and also on Jordan's medium, must be rather considered as a gasogenic intermedius type. d Other biological characteristics, such as activity upon milk, milk-whey, dextrin, etc., accepted by several specialists, appeared as of no practical value to the distinction of the different types of our strains. e The strains kept their properties invariable during all the time of our investigations. The hypothesis of a possible transformation of one type into another has not been confirmed in any of the studied strains and this justifies the idea of independence of each one of the accepted types. f Some discrepances in fermentation of maltose as found by several authors in microorganisms of this group, have been not confirmed in this paper. g In

  19. Intensity techniques

    DEFF Research Database (Denmark)

    Jacobsen, Finn

    2008-01-01

    The Handbook of Signal Processing in Acoustics will compile the techniques and applications of signal processing as they are used in the many varied areas of Acoustics. The Handbook will emphasize the interdisciplinary nature of signal processing in acoustics. Each Section of the Handbook...... from different areas, will find the self-contained chapters accessible and will be interested in the similarities and differences between the approaches and techniques used in different areas of acoustics....

  20. An indirect fluorescent antibody technique for detection of anti-Dermatophilus congolensis antibodies in sheep.

    Science.gov (United States)

    Hermoso De Mendoza, J; Nieto, C G; Arenas, A; Alonso, J M; Rey, J; Gil, M C; Anton, J; Cardenal, A; Hermoso De Mendoza, M

    1994-05-01

    An indirect fluorescent antibody (IFA) technique has been developed for detection of anti-Dermatophilus antibodies in sheep. Sera from 25 bacteriologically confirmed clinically affected sheep and from 10 negative non affected lambs were used. Whole cell antigen from brain heart infusion cultures of D. congolensis was used and all sera were tested in the same way for cross-reactivity against antigens obtained from cultures of Actinomyces viscosus, Micrococcus luteus, Nocardia asteroides, and Corynebacterium pseudotuberculosis. Sera from Dermatophilus-infected sheep gave positive results with D. congolensis antigen and negative results with the antigens from other bacteria. The whole cell antigens employed were simple to prepare and easy to recognise by microscopy. Cross-reactivity was further tested using the D. congolensis culture whole cell antigen and 3 sera from sheep with bacteriologically confirmed natural infections due to Corynebacterium pseudotuberculosis, Actinomyces pyogenes and Nocardia asteroides. None of these sera showed positive reactions. The authors recommend this technique for serological surveys and research on dermatophilosis.

  1. Radionuclide techniques

    International Nuclear Information System (INIS)

    Alazraki, N.

    1988-01-01

    During approximately 25 years of experience with bone scanning, broad expansions in the clinical impact of radionuclide imaging on disorders of bones, joints, and soft tissues have occurred. This increased impact had its groundwork laid by advances in radiopharmaceuticals and instruments that are used for bone imaging. The progression from /sup 85/Sr- to /sup 99m/Tc-labeled phosphate and phosphonate compounds, coupled with the advance from crude rectilinear scanning techniques to whole body scintillation imaging with large crystal cameras and sophisticated electronics, has encourage use of radionuclide techniques in the evaluation of muscoloskeletal diseases. Many clinical studies have documented the high degree of sensitivity of the bone scan compared with that of the radiograph in detecting osseous and articular abnormalities

  2. Astrophysical techniques

    CERN Document Server

    Kitchin, CR

    2013-01-01

    DetectorsOptical DetectionRadio and Microwave DetectionX-Ray and Gamma-Ray DetectionCosmic Ray DetectorsNeutrino DetectorsGravitational Radiation Dark Matter and Dark Energy Detection ImagingThe Inverse ProblemPhotographyElectronic ImagingScanningInterferometrySpeckle InterferometryOccultationsRadarElectronic ImagesPhotometryPhotometryPhotometersSpectroscopySpectroscopy SpectroscopesOther TechniquesAstrometryPolarimetrySolar StudiesMagnetometryComputers and The Internet.

  3. Aseptic technique.

    Science.gov (United States)

    Bykowski, Tomasz; Stevenson, Brian

    2008-11-01

    This chapter describes common laboratory procedures that can reduce the risk of culture contaminations (sepsis), collectively referred as "aseptic technique." Two major strategies of aseptic work are described: using a Bunsen burner and a laminar flow hood. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and dispensing aliquots, preparing growth media, and inoculating, passaging, and spreading microorganisms on petri dishes. Copyright 2008 by John Wiley & Sons, Inc.

  4. The answer of the Bacteriology Laboratory to new clinical needs. Rapid sepsis diagnotics at the Novara hospital

    Directory of Open Access Journals (Sweden)

    Vesselina Kroumova

    2012-06-01

    Full Text Available Faster microbiological responses are increasingly necessary in modern medicine and the Laboratory of Microbiology must be equipped in this sense. New instrumentation and, above all, a new approach by the Clinical Microbiologist that puts a focus on the real needs of the patient before the microbiological may allow for significantly improving the TAT of these diagnostics. The use of both new methodologies, new tools and revisited old technologies may mean less these days as it was obtained at the Laboratory of Microbiology and Virology of Novara, where the combined use of molecular biology techniques, and mass spectrometry techniques rapid growth have allowed for more than 36 hours to shorten the response time by positivization of blood cultures. Such an approach allows an important support to the clinician with obvious benefits for the patient.

  5. Bacteriological incidence in pneumonia patients with pulmonary emphysema: a bacterial floral analysis using the 16S ribosomal RNA gene in bronchoalveolar lavage fluid

    Directory of Open Access Journals (Sweden)

    Naito K

    2017-07-01

    Full Text Available Keisuke Naito,1 Kei Yamasaki,1 Kazuhiro Yatera,1 Kentaro Akata,1 Shingo Noguchi,1 Toshinori Kawanami,1 Kazumasa Fukuda,2 Takashi Kido,1 Hiroshi Ishimoto,3 Hiroshi Mukae3 1Department of Respiratory Medicine, 2Department of Microbiology, University of Occupational and Environmental Health, Japan, Kitakyushu City, Fukuoka, 3Second Department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki City, Nagasaki, Japan Abstract: Pulmonary emphysema is an important radiological finding in chronic obstructive pulmonary disease patients, but bacteriological differences in pneumonia patients according to the severity of emphysematous changes have not been reported. Therefore, we evaluated the bacteriological incidence in the bronchoalveolar lavage fluid (BALF of pneumonia patients using cultivation and a culture-independent molecular method. Japanese patients with community-acquired pneumonia (83 and healthcare-associated pneumonia (94 between April 2010 and February 2014 were evaluated. The BALF obtained from pneumonia lesions was evaluated by both cultivation and a molecular method. In the molecular method, ~600 base pairs of bacterial 16S ribosomal RNA genes in the BALF were amplified by polymerase chain reaction, and clone libraries were constructed. The nucleotide sequences of 96 randomly selected colonies were determined, and a homology search was performed to identify the bacterial species. A qualitative radiological evaluation of pulmonary emphysema based on chest computed tomography (CT images was performed using the Goddard classification. The severity of pulmonary emphysema based on the Goddard classification was none in 47.4% (84/177, mild in 36.2% (64/177, moderate in 10.2% (18/177, and severe in 6.2% (11/177. Using the culture-independent molecular method, Moraxella catarrhalis was significantly more frequently detected in moderate or severe emphysema patients than in patients with no or mild emphysematous changes. The

  6. Clinical, bacteriological, and histopathological characteristics of newly detected children with leprosy: A population based study in a defined rural and urban area of Maharashtra, Western India

    Directory of Open Access Journals (Sweden)

    Vanaja P Shetty

    2013-01-01

    Full Text Available Background: Leprosy has been a major public-health problem in many developing countries for centuries. According to the National Leprosy Elimination Programme report of March 2012, there were a total of about 0.13 million cases of leprosy in India, 9.7% of which were children. Numerous studies have investigated child leprosy amongst reported cases however, studies pertaining to proportion and characteristics of undetected childhood cases in the community are very few. Aim: To examine the clinical, bacteriological, and histopathological characteristics of newly detected child leprosy cases in the community. Methods: The population survey conducted from June to September 2007 and the defined rural areas, which included five primary health centers of Panvel Taluka, in Raigad district and urban areas, which included M-east ward of the municipal corporation of greater Mumbai of western Maharashtra, India. Results: House-to-house survey yielded 32 and 37 so far, undetected child cases of leprosy in the rural and urban region, and the prevalence rate was 10.5 and 1.5 per 10,000, respectively. The age of child leprosy cases detected, ranged from 3 to 14 years with a mean of 10.06 ± 3.35 years in the rural and 9.97 ± 3.12 years in the urban area. Most of the cases were paucibacillary (62%. A large proportion of children (49% had single skin lesion (SSL. Of the 19 SSL cases examined histopathologically, 15 (99% showed features of borderline tuberculoid, 1 (5% borderline lepromatous and 3 (16% had indeterminate type of leprosy. Tuberculoid leprosy was not seen in any, indicating less likelihood of self-healing. Overall, three cases had deformity (grade 1 = 1 and grade 2 = 2 and 31% of multibacillary cases were smear positive. Conclusion: The clinical, bacteriological, and histopathological characteristics of newly detected child cases in the community evidently indicate the grave nature of the problem of undetected child leprosy, recent active

  7. Presentation Technique

    International Nuclear Information System (INIS)

    Froejmark, M.

    1992-10-01

    The report presents a wide, easily understandable description of presentation technique and man-machine communication. General fundamentals for the man-machine interface are illustrated, and the factors that affect the interface are described. A model is presented for describing the operators work situation, based on three different levels in the operators behaviour. The operator reacts routinely in the face of simple, known problems, and reacts in accordance with predetermined plans in the face of more complex, recognizable problems. Deep fundamental knowledge is necessary for truly complex questions. Today's technical status and future development have been studied. In the future, the operator interface will be based on standard software. Functions such as zooming, integration of video pictures, and sound reproduction will become common. Video walls may be expected to come into use in situations in which several persons simultaneously need access to the same information. A summary of the fundamental rules for the design of good picture ergonomics and design requirements for control rooms are included in the report. In conclusion, the report describes a presentation technique within the Distribution Automation and Demand Side Management area and analyses the know-how requirements within Vattenfall. If different systems are integrated, such as geographical information systems and operation monitoring systems, strict demands are made on the expertise of the users for achieving a user-friendly technique which is matched to the needs of the human being. (3 figs.)

  8. Utilisation de techniques bactériologiques et biochimiques pour l'étude du biofilm bactérien Use of Bacteriological and Biochemical Techniques for Analyzing Bacterial Biofilms

    Directory of Open Access Journals (Sweden)

    Fera P.

    2006-11-01

    Full Text Available La formation d'un voile biologique sur des surfaces métalliques exposées en milieu marin se traduit par une augmentation de la résistance au transfert thermique et à l'écoulement du fluide, mais aussi par un risque d'initiation de corrosion localisée due à la présence et à l'activité métabolique des microorganismes constituant ce biofilm. L'utilisation conjointe des méthodes microbiologiques et biochimiques permet une meilleure compréhension, à la fois des mécanismes de formation de ce biofilm et de la structure de la communauté bactérienne qui le constitue. Ces méthodes ont déjà été utilisées simultanément lors de travaux visant à étudier la corrosion bactérienne, la colonisation bactérienne de surfaces métalliques exposées à une eau de mer circulante ou l'action de biocides sur la formation du biofilm. Materials exposed to seawater typically develop a layer of attached microorganisms that is referred to as biofouling. This biofouling can induce heat transfer resistance and fluid frictional resistance and can also initiate localized corrosion due to the metabolic activity of microorganisms making up the biofilm. Microbiological and biochemical methods can be used to gain a better understanding of the mecanisms of biofilm formation and the structure of the attached bacteria community. These methods can be applied in many circumstances, as shown by bacterial corrosion analysis, colonization of surfaces exposed to flowing seawater or use of biocides against sessile bacteria.

  9. Qualitative bacteriology in malignant wounds--a prospective, randomized, clinical study to compare the effect of honey and silver dressings

    DEFF Research Database (Denmark)

    Lund-Nielsen, Betina; Adamsen, Lis; Gottrup, Finn

    2011-01-01

     Between 5% and 10% of cancer patients develop malignant wounds. In vitro and some clinical studies suggest that silver- or honey-coated dressings may have an antibacterial effect in nonmalignant wounds, but their possible antibacterial effect in malignant wounds remains unknown...... antibacterial effect of the honey or silver dressing could not be confirmed in these malignant wounds. Routine wound swabbing of malignant wounds is of little value and should be restricted to cases where signs of infection requiring antibiotic intervention are observed or where resistant organisms require....... A prospective, randomized, single-blind controlled clinical study was conducted to evaluate the bacteriology of malignant wounds and compare the effect of a honey-coated (Group A) to a silver-coated (Group B) dressing on the qualitative bacteriology of malignant wounds. All wound interventions were performed...

  10. Qualitative bacteriology in malignant wounds--a prospective, randomized, clinical study to compare the effect of honey and silver dressings

    DEFF Research Database (Denmark)

    Lund-Nielsen, Betina; Adamsen, Lis; Gottrup, Finn

    2011-01-01

    Between 5% and 10% of cancer patients develop malignant wounds. In vitro and some clinical studies suggest that silver- or honey-coated dressings may have an antibacterial effect in nonmalignant wounds, but their possible antibacterial effect in malignant wounds remains unknown. A prospective......, randomized, single-blind controlled clinical study was conducted to evaluate the bacteriology of malignant wounds and compare the effect of a honey-coated (Group A) to a silver-coated (Group B) dressing on the qualitative bacteriology of malignant wounds. All wound interventions were performed by the same...... antibacterial effect of the honey or silver dressing could not be confirmed in these malignant wounds. Routine wound swabbing of malignant wounds is of little value and should be restricted to cases where signs of infection requiring antibiotic intervention are observed or where resistant organisms require...

  11. Qualitative bacteriology in malignant wounds--a prospective, randomized, clinical study to compare the effect of honey and silver dressings

    DEFF Research Database (Denmark)

    Lund-Nielsen, Betina; Adamsen, Lis; Gottrup, Finn

    2011-01-01

    . A prospective, randomized, single-blind controlled clinical study was conducted to evaluate the bacteriology of malignant wounds and compare the effect of a honey-coated (Group A) to a silver-coated (Group B) dressing on the qualitative bacteriology of malignant wounds. All wound interventions were performed...... Between 5% and 10% of cancer patients develop malignant wounds. In vitro and some clinical studies suggest that silver- or honey-coated dressings may have an antibacterial effect in nonmalignant wounds, but their possible antibacterial effect in malignant wounds remains unknown...... years, range 47-92) consented to participate and completed the 4-week study. The majority were women (88%) with breast cancer (79%). No statistically significant differences were found between the type and number of different wound pathogens in the wounds during the course of the study or between Group...

  12. Terahertz Techniques

    CERN Document Server

    Bründermann, Erik; Kimmitt, Maurice FitzGerald

    2012-01-01

    Research and development in the terahertz portion of the electromagnetic spectrum has expanded very rapidly during the past fifteen years due to major advances in sources, detectors and instrumentation. Many scientists and engineers are entering the field and this volume offers a comprehensive and integrated treatment of all aspects of terahertz technology. The three authors, who have been active researchers in this region over a number of years, have designed Terahertz Techniques to be both a general introduction to the subject and a definitive reference resource for all those involved in this exciting research area.

  13. [The Austrian Red Cross and Austrian bacteriologists in the Balkan wars 1912/13 - Centenary of the first application of the bacteriology in theatres of war].

    Science.gov (United States)

    Flamm, Heinz

    2012-04-01

    When Austria joined the Geneva Convention the "Patriotischer Hilfsverein" (Patriotic Aid Society) which was founded for the concerns of wounded soldiers, was named "Austrian Society of the Red Cross". It had to stand its first big test in 1912 in the first Balkan war; military surgeons and bacteriologists were deployed to all warring states. The cholera dominated under the infectious diseases among the various forces and the civilian populations. Upon request of the Bulgarian king renowned bacteriologists of the University of Vienna were dispatched. Their work presented the first action of bacteriology for disease control on theatres of war. The great success of the surgical and hygienic measures initiated in 1912 a reform of the Austrian Red Cross. In 1913 Austria made a detailed application to the International Committee of the Red Cross in order to extend the Geneva Convention to war epidemics. The Committee forwarded and recommended this application to all member states, however, the outbreak of the First World War then prevented its resolution.

  14. [Evaluation of the methodological quality of the Rémic (microbiology guidelines - bacteriology and mycology) of the Société française de microbiologie].

    Science.gov (United States)

    Fonfrède, Michèle; Couaillac, Jean Paul; Augereau, Christine; De Moüy, Danny; Lepargneur, Jean Pierre; Szymanowicz, Anton; Watine, Joseph

    2011-01-01

    We have evaluated the methodological quality of the Rémic (microbiology guidelines - bacteriology and mycology) of the Société française de microbiologie (edition2007), using to AGREE criteria, which are consensual at an international level, in particular at the the World Health Organisation (WHO) and at the European Union. The methodological quality of the Rémic appears to be sub-optimal. These shortcomings in quality are mainly observed in AGREE domain n° 5 (applicability), in AGREE item n° 5 (patients' opinions were not considered), and in AGREE item n° 23 (conflicts of interest were not declared). The users of the Rémic must be aware of these few methodological shortcomings in order for them to be careful before they put its recommendation in practice. In conclusion, we advise the editors of the Rémic to insert at least a methodological chapter in their next edition.

  15. Bacteriological quality of weaning food and drinking water given to children of market women in Nigeria: implications for control of diarrhoea.

    Science.gov (United States)

    Iroegbu, C U; Ene-Obong, H N; Uwaegbute, A C; Amazigo, U V

    2000-12-01

    Bacteriological quality of weaning food and drinking water given to 2 groups of children aged leftover foods also had significantly higher bacterial load than the freshly-cooked food. Coliform count varied significantly with source of drinking water. Poor hygiene standard (inferred from bacterial contamination) was generally observed among mothers weaning < or = 2-year-old children, while they were engaged in trading activities in the market, thus exposing their children to high risk of diarrhoea. Hygiene was significantly poorer in weaning of children left at home in the care of older siblings or house-helps. This implies that, in spite of their trading activities in the market, mothers still take better care of their babies than the older siblings or house-helps who may be inexperienced. These mothers may need education on childcare and food hygiene to suit to their trading activities, for example, during their monthly meetings. There is also a need to establish ORT (oral rehydration therapy) corners in the markets as part of the municipal services. This can be used not only for efficient and quick management of diarrhoea in the market but also for reinforcing hygiene education.

  16. Post-exposure serological and bacteriological responses of water buffalo (Bubalus bubalis) to Brucella abortus biovar 1 following vaccination with Brucella abortus strain RB51.

    Science.gov (United States)

    Diptee, M D; Asgarali, Z; Campbell, M; Fosgate, G; Adesiyun, A A

    2007-12-01

    Serological and bacteriological responses to Brucella abortus biovar 1 following vaccination with B. abortus strain RB51 (RB51) were evaluated in thirty domestic water buffalo (Bubalus bubalis) randomly divided into five treatment groups. Groups I to V received, respectively, the recommended dose (RD) of RB51 vaccine once, RD twice 4 weeks apart, double RD once, double RD twice 4 weeks apart, and saline once (control). Vaccination did not result in a serological response. Experimental animals released 27 weeks post initial inoculation (27 PIIW) into a brucellosis-positive herd failed to seroconvert after 29 weeks. Experimental challenge commenced at 57 PIIW. All animals received B. abortus biovar 1 intraconjunctivally at 0, 5 and 9 weeks post experimental exposure (PEEW). Serum samples collected at 4, 8 and 13 PEEW were negative. At 16 PEEW all animals received B. abortus biovar 1 subcutaneously (SC), and all seroconverted by 20 PEEW. Five of twenty-six animals were positive for Brucella infection on bacterial culture. Brucella abortus biovar 1 was isolated from three animals; B. abortus RB51 was isolated from two. Treatment group, age and sex had no effect on the isolation of Brucellae (P>0.05).

  17. Cervicovaginal Bacteriology and Antibiotic Sensitivity Patterns among Women with Premature Rupture of Membranes in Mulago Hospital, Kampala, Uganda: A Cross-Sectional Study

    Directory of Open Access Journals (Sweden)

    Milton W. Musaba

    2017-01-01

    Full Text Available Background. A 2013 Cochrane review concluded that the choice of antibiotics for prophylaxis in PROM is not clear. In Uganda, a combination of oral erythromycin and amoxicillin is the 1st line for prophylaxis against ascending infection. Our aim was to establish the current cervicovaginal bacteriology and antibiotic sensitivity patterns. Methods. Liquor was collected aseptically from the endocervical canal and pool in the posterior fornix of the vagina using a pipette. Aerobic cultures were performed on blood, chocolate, and MacConkey agar and incubated at 35–37°C for 24–48 hrs. Enrichment media were utilized to culture for GBS and facultative anaerobes. Isolates were identified using colonial morphology, gram staining, and biochemical analysis. Sensitivity testing was performed via Kirby-Bauer disk diffusion and dilution method. Pearson’s chi-squared (χ2 test and the paired t-test were applied, at a P value of 0.05. Results. Thirty percent of the cultures were positive and over 90% were aerobic microorganisms. Resistance to erythromycin, ampicillin, cotrimoxazole, and ceftriaxone was 44%, 95%, 96%, and 24%, respectively. Rupture of membranes (>12 hrs, late preterm, and term PROM were associated with more positive cultures. Conclusion. The spectrum of bacteria associated with PROM has not changed, but resistance to erythromycin and ampicillin has increased.

  18. Combinatorial techniques

    CERN Document Server

    Sane, Sharad S

    2013-01-01

    This is a basic text on combinatorics that deals with all the three aspects of the discipline: tricks, techniques and theory, and attempts to blend them. The book has several distinctive features. Probability and random variables with their interconnections to permutations are discussed. The theme of parity has been specially included and it covers applications ranging from solving the Nim game to the quadratic reciprocity law. Chapters related to geometry include triangulations and Sperner's theorem, classification of regular polytopes, tilings and an introduction to the Eulcidean Ramsey theory. Material on group actions covers Sylow theory, automorphism groups and a classification of finite subgroups of orthogonal groups. All chapters have a large number of exercises with varying degrees of difficulty, ranging from material suitable for Mathematical Olympiads to research.

  19. Industrial technique

    International Nuclear Information System (INIS)

    Jabsen, F.S.

    1975-01-01

    An illustrative embodiment of the invention discloses a technique for disassembling a nuclear reactor fuel element without destroying the individual fuel pins and other structural components from which the element is assembled. A traveling bridge and trolley that span a water-filled spent fuel storage pool support a strongback. The strongback is under water and provides a working surface on which the spent fuel element is placed for inspection and for the manipulation that is associated with disassembly and assembly. To remove, in a non-destructive manner, the grids that hold the fuel pins in the proper relative positions within the element, bars are inserted through apertures in the grids with the aid of special tools. These bars are rotated to flex the adjacent grid walls and, in this way relax the physical engagement between protruding portions of the grid walls and the associated fuel pins. With the grid structure so flexed to relax the physical grip on the individual fuel pins, these pins can be withdrawn for inspection or replacement as necessary without imposing a need to destroy fuel element components. (U.S.)

  20. Recovery methods for detection and quantification of Campylobacter depend on meat matrices and bacteriological or PCR tools.

    Science.gov (United States)

    Fosse, J; Laroche, M; Rossero, A; Fédérighi, M; Seegers, H; Magras, C

    2006-09-01

    Campylobacter is one of the main causes of human foodborne bacterial disease associated with meat consumption in developed countries. Therefore, the most effective approach for recovery and detection of Campylobacter from meat should be determined. Two hundred ninety pork skin and chine samples were inoculated with Campylobacter jejuni NCTC 11168 and two strains of Campylobacter coli. Campylobacter cells were then recovered from suspensions and enumerated by direct plating. Campylobacter recovery was evaluated by comparing results for two methods of sample collection (swabbing and mechanical pummeling) and three recovery fluids (peptone water, 5% glucose serum, and demineralized water). End-point multiplex PCR was performed to evaluate the compatibility of the recovery fluids with direct PCR detection techniques. Mean recovery ratios differed significantly between pork skin and chine samples. Ratios were higher for mechanical pummeling (0.53 for pork skin and 0.49 for chine) than for swabbing (0.31 and 0.13, respectively). For pork skin, ratios obtained with peptone water (0.50) and with glucose serum (0.55) were higher than those obtained with demineralized water (0.16). Significant differences were not observed for chine samples. Direct multiplex PCR detection of Campylobacter was possible with pork skin samples. The tools for Campylobacter recovery must be appropriate for the meat matrix to be evaluated. In this study, less than 66% of inoculated Campylobacter was recovered from meat. This underestimation must be taken into account for quantitative risk analysis of Campylobacter infection.

  1. Bacteriological safety assessment, hygienic habits and cross-contamination risks in a Nigerian urban sample of household kitchen environment.

    Science.gov (United States)

    Ejechi, Bernard O; Ochei, Ono P

    2017-06-01

    Urban household kitchen environment was assessed for safety by determining their levels of indicator bacteria, hygienic habits and risk of cross-contamination. Household kitchens (60) were selected in Warri Town, Nigeria, by the multi-stage sampling technique. Contact surfaces, water and indoor kitchen air were analysed for aerobic plate counts, total and faecal coliforms using Nutrient and McConkey media by swab/rinse method, membrane filtration and sedimentation methods, respectively. Hygienic habits and risk of cross-contamination were assessed with structured questionnaire which included socio-demographic variables. On the basis of median counts, the prevalence of high counts (log cfu/cm 2 /m 3 /100 mL) of aerobic plate counts (>3.0), total coliforms (>1.0) and faecal coliforms (>0) on contact surfaces and air was high (58.0-92.0%), but low in water (30.0-40.0%). Pots, plates and cutleries were the contact surfaces with low counts. Prevalence of poor hygienic habits and high risk of cross-contamination was 38.6 and 67.5%, respectively. Education, occupation and kitchen type were associated with cross-contamination risk (P = 0.002-0.022), while only education was associated with hygienic habits (P = 0.03). Cross-contamination risk was related (P = 0.01-0.05) to aerobic plate counts (OR 2.30; CL 1.30-3.17), total coliforms (OR 5.63; CL 2.76-8.25) and faecal coliforms (OR 4.24; CL 2.87-6.24), while hygienic habit was not. It can be concluded that urban household kitchens in the Nigerian setting are vulnerable to pathogens likely to cause food-borne infections.

  2. Bacteriological evaluation of fresh chicken sausage submitted to gamma radiation; Avaliacao bacteriologica da linguica de frango Frescal submetida a radiacao gama

    Energy Technology Data Exchange (ETDEWEB)

    Borges, Alexandre

    2002-07-01

    light of the lack of present data on the irradiation of fresh chicken sausage, the objective of this study is to evaluate determine the efficiency of this method as a process for reducing the microbial load via measurement of the bacteriology of the product before and after gamma radiation. 45 sample of chicken sausage(acquired directly from the poultry industry) were prepared divided in 3 treatments: the first is a control, the second and third using application of 1,5 and 3,0 kGy, respectively. The chicken sausages irradiated with a dose of 1,5 kGy did not have, statistically significant results apparent in the bacteriological measures. Samples irradiated with 3,0 kGy did experience statistically significant reductions. It is concluded that the irradiating the product with a dose of 3,0 kGy is more effective for the reduction of bacterial population, with the data in these trials indicating a 95,3% reduction in total coliforms, 100% removal of fecal coliforms, and 84,0% reduction of mesophiles, relative to the control samples. (author)

  3. Techniques de combustion Combustin Techniques

    Directory of Open Access Journals (Sweden)

    Perthuis E.

    2006-11-01

    Full Text Available L'efficacité d'un processus de chauffage par flamme est étroitement liée à la maîtrise des techniques de combustion. Le brûleur, organe essentiel de l'équipement de chauffe, doit d'une part assurer une combustion complète pour utiliser au mieux l'énergie potentielle du combustible et, d'autre part, provoquer dans le foyer les conditions aérodynamiques les plus propices oux transferts de chaleur. En s'appuyant sur les études expérimentales effectuées à la Fondation de Recherches Internationales sur les Flammes (FRIF, au Groupe d'Étude des Flammes de Gaz Naturel (GEFGN et à l'Institut Français du Pétrole (IFP et sur des réalisations industrielles, on présente les propriétés essentielles des flammes de diffusion aux combustibles liquides et gazeux obtenues avec ou sans mise en rotation des fluides, et leurs répercussions sur les transferts thermiques. La recherche des températures de combustion élevées conduit à envisager la marche à excès d'air réduit, le réchauffage de l'air ou son enrichissement à l'oxygène. Par quelques exemples, on évoque l'influence de ces paramètres d'exploitation sur l'économie possible en combustible. The efficiency of a flame heating process is closely linked ta the mastery of, combustion techniques. The burner, an essential element in any heating equipment, must provide complete combustion sa as to make optimum use of the potential energy in the fuel while, at the same time, creating the most suitable conditions for heat transfers in the combustion chamber. On the basis of experimental research performed by FRIF, GEFGN and IFP and of industrial achievements, this article describesthe essential properties of diffusion flames fed by liquid and gaseous fuels and produced with or without fluid swirling, and the effects of such flames on heat transfers. The search for high combustion temperatures means that consideration must be given to operating with reduced excess air, heating the air or

  4. Liver function and bacteriology of organs in broiler inoculated with nalidixic acid-resistant Salmonella Typhimurium and treated with organic acids

    Directory of Open Access Journals (Sweden)

    Tatiane M. Rocha

    2013-07-01

    Full Text Available AbAns etxrpaecritment was carried out with 630 one-day-old chicks to evaluate the effects of organic acids when birds were experimentally inoculated with Salmonella Typhimurium. Liver damage and the persistence of the bacterium in the organs were evaluated as well. Broilers were distributed in a completely randomised experimental design in a 3×2 factorial arrangement of six treatments with seven replicates of 15 birds each. Birds were inoculated with saline solution or the bacterium via gavage at 1 day of age, or were offered a feed containing or not the organic acid blend for the period of 7 to 14 days of age. A dose of 5.0x102 colony-forming units (CFU/0.5 mL of Salmonella Typhimurium was used for inoculation both via gavage and feed. The parameters evaluated are weight, liver histopathology, liver and serum biochemistry, and bacteriological analyses of the caeca, crop, spleen, and liver and heart pool. At 21 and 28 days of age, the liver of the non-inoculated groups was significantly lighter as compared to the other treatments. Birds fed organic acids presented lower bacterial isolation rates in all organs tested. Birds inoculated in the crop and treated with organic acids presented lower E. coli CFU counts (P<0.05. Birds inoculated with Salmonella presented significant changes (P<0.05 in liver enzymes, as detected by serum biochemistry, and in liver histopathology. It was concluded that organic acids effectively controlled Salmonella Typhimurium and did not cause any liver damage.

  5. Bacteriological profile of diabetic foot ulcer with special reference to drug-resistant strains in a tertiary care center in North-East India

    Directory of Open Access Journals (Sweden)

    Sudhir K Jain

    2017-01-01

    Full Text Available Aim: This study was carried out to determine the bacteriological profile of infected diabetic foot ulcers (DFUs and the antibiotic resistance pattern from the isolates. An attempt was made to suggest an empiric antibiotic regimen to treat such patients. Materials and Methods: Tissue samples were collected from 150 patients between February 2015 and January 2016 with DFUs under aseptic precautions and they were processed as per the Clinical and Laboratory Standards Institute guidelines. Results: A total of 185 bacterial isolates were obtained from 150 persons with diabetic and with foot ulcers. The age group of these persons ranged from 35 to 80 years and the maximum number of persons with DFUs was in the age group of 60–65 years. Among the isolates, Gram-negative bacilli were isolated in 112/185 (61% and Gram-positive cocci in 73/185 (39% cases. The most common isolate was Staphylococcus spp. 46 (25%, followed by Escherichia coli (20% and Enterococcus spp. (15%. The antibiotic sensitivity profile of the bacteria was also studied. Among the isolates, 59/112 (53% of the Gram-negative bacilli were extended spectrum beta-lactamase producers, 19/46 (41% were methicillin-resistant Staphylococcus aureus, and 5/27 (19% were vancomycin-resistant Enterococcus. Conclusions: This study showed a preponderance of multidrug-resistant strains among the isolates from the DFUs. Knowledge on the antibiotic sensitivity pattern of the isolates will be helpful in determining the drugs for the empirical treatment of diabetic ulcers. Thus, indiscriminate use of antibiotics and chances of subsequent development of antibiotic resistance can also be reduced.

  6. [Analysis of a proposal for changing the educational paradigm in bacteriology and virology. Facultad de Ciencias Médicas, Universidad Nacional de Córdoba].

    Science.gov (United States)

    Márquez, E; González, S; López, T; Giayetto, V; Littvik, A; Cannistraci, R; Pavan, J

    2000-01-01

    The productive character of the scientific thought points out a methodological means that include the demand of the students' job about the information and not his mere reception and reproduction. It is essential to give the students the opportunity of discovering the cognitive processes used in the production of the scientific knowledge. In this work, we present the result of the starting of a workshop dynamic in a basic subject, the students' answers, and the analysis of the subject. In the Chair of Medical Bacteriology and Virology of the School of Medical Sciences, the National University of Córdoba, 1700 students attended classes in 1997. The subject was developed with the activities that worked the same contents from two different learning conceptions: (I) workshop activity, non obligatory, constructive, and (II) theorico-practical activity, obligatory and traditional. Two voluntary and anonymous interviews were done about the valuation that the student gave to these two activities and their basis, one in the middle and the other at the end of the course. 90.55% classified the traditional activity as positive, and 9.45% as negative. Regarding the workshop activity, the 60.5% classified it as positive and the 39.5% as negative. The same developed content with two activities made possible the analysis of the impact that the two different methodologies produced on the students. The student's answer to the traditional activity was better than the workshop activity (p < 0.001). The differences in the student's acceptance between the two option revealed the major difficulty of an alternative methodological strategy in the current educational model.

  7. Calidad bacteriológica del agua para consumo humano en zonas de alta marginación de Chiapas Bacteriological quality of drinking water in areas of high levels of poverty in Chiapas, Mexico

    Directory of Open Access Journals (Sweden)

    Héctor Javier Sánchez-Pérez

    2000-09-01

    Full Text Available OBJETIVO: Analizar la calidad bacteriológica del agua (CBA para consumo humano y su relación con diarreas y enteroparasitosis en niños de 1 a 14 años en comunidades de alta marginación socioeconómica de Chiapas, México. MATERIAL Y MÉTODOS: En una muestra aleatoria de 99 viviendas de la Región Fronteriza de Chiapas, de marzo a septiembre de 1998, se recolectaron muestras de agua para consumo humano, se indagó sobre diarreas en los últimos 15 días y se recolectaron muestras de heces de 322 niños de 1 a 14 años. La CBA se determinó mediante la técnica de filtración por membranas y las enteroparasitosis por el método de Faust. Se utilizó el estadístico ji² para el análisis de la CBA con relación a los diversos factores analizados. RESULTADOS: Sólo 31% de las muestras de agua fueron aptas para consumo humano. La CBA y la presencia de diarreas referida por las madres de los menores no mostraron asociación. Los niños con mala CBA en sus viviendas mostraron mayor prevalencia de Entamoeba histolytica y mayor tendencia a estar parasitados. CONCLUSIONES: Es necesario desarrollar medidas que mejoren la CBA y campañas de educación que incrementen el uso de agua hervida, su manejo adecuado y el cuidado de las fuentes de abastecimiento comunitarias.OBJECTIVE: To analyze the bacteriological quality of drinking water (BQDW and its association with diarrhoea and intestinal parasites in children 1 to 14 years old, in areas of high levels of poverty in Chiapas, Mexico. MATERIAL AND METHODS: From March to September 1998, drinking water was collected from a random sample of 99 households in the Border Region of Chiapas, Mexico; data on diarrhoeal disease (in the past 15 days were collected and stool testing for intestinal parasites was performed in children 1 to 14 years old (n= 322. The BQDW was determined by the filtration membrane technique. Intestinal parasitic infections were determined by the Faust Method. The chi² statistic was

  8. Diagnóstico bacteriológico de la tuberculosis en Argentina: resultados de una encuesta nacional Bacteriological diagnosis of tuberculosis in Argentina: results of a national survey

    Directory of Open Access Journals (Sweden)

    María Susana Imaz

    2007-04-01

    Full Text Available Con el objetivo de evaluar recursos, prácticas y medidas de bioseguridad utilizados en el diagnóstico bacteriológico de tuberculosis y determinar la utilidad y alcance de las técnicas empleadas, se llevó adelante una encuesta transversal a laboratorios del sector público de Argentina utilizando un cuestionario estructurado. El análisis de los resultados mostró que la densidad de centros de microscopía y cultivo se encuentra al nivel o encima de lo recomendado por Organización Mundial de la Salud. El programa de control de calidad de microscopía cubre mayoritariamente los laboratorios con alta carga de trabajo. El promedio de baciloscopías/paciente fue bajo (1,6. El 25% de los laboratorios de cultivo empleaban protección respiratoria inadecuada. El aporte del cultivo a la confirmación de casos pulmonares en las jurisdicciones estuvo asociado a la proporción de muestras cultivadas. Globalmente, el porcentaje de pacientes pulmonares baciloscopía-negativa que fue confirmado bacteriológicamente fue de 18.9%; mientras que la may-oría de los casos extrapulmonares se confirmó sólo por cultivo (71,3%. Es prioritario aún el incremento del número de esputos estudiados por baciloscopía y cultivo, así como aumentar la cobertura de programa de control de calidad de microscopía; es fundamental proveer al personal de laboratorio de adecuada protección respiratoria y evaluación médica regular.In order to assess laboratory resources, practices, and biosafety measures during mycobacterial testing and determine the usefulness and scope of mycobacterial techniques, a cross-sectional survey of public laboratories was conducted in Argentina using a structured questionnaire. Sputum smear analysis showed that both smear and culture testing centers are being provided at or above the WHO recommended density for such facilities. The microscopy quality assessment program covered most high-demand laboratories. Mean number of sputum smears per

  9. Environmental impact on the bacteriological quality of domestic water supplies in Lagos, Nigeria Impacto ambiental sobre a qualidade bacteriológica do abastecimento domiciliar de água em Lagos, Nigéria

    Directory of Open Access Journals (Sweden)

    L Egwari

    2002-08-01

    Full Text Available OBJECTIVE: To assess the impact of town planning, infrastructure, sanitation and rainfall on the bacteriological quality of domestic water supplies. METHODS: Water samples obtained from deep and shallow wells, boreholes and public taps were cultured to determine the most probable number of Escherichia coli and total coliform using the multiple tube technique. Presence of enteric pathogens was detected using selective and differential media. Samples were collected during both periods of heavy and low rainfall and from municipalities that are unique with respect to infrastructure planning, town planning and sanitation. RESULTS: Contamination of treated and pipe distributed water was related with distance of the collection point from a utility station. Faults in pipelines increased the rate of contamination (pOBJETIVO: Avaliar o impacto do planejamento urbano, da infra-estrutura, do saneamento e dos índices pluviométricos sobre a qualidade bacteriológica do abastecimento domiciliar de água. MÉTODOS: Foi realizada cultura de amostras de água obtida de poços superficiais e profundos, fossos e água corrente de bicas públicas para determinar o número mais provável de Escherichia coli e coliformes totais por meio da técnica de múltiplos tubos. Patógenos entéricos foram detectados pelo uso de meios diferenciais e seletivos. Amostras foram coletadas durante os períodos de seca e de chuvas intensas em municípios com características singulares de infra-estrutura, planejamento urbano e saneamento. RESULTADOS: A contaminação de água tratada ou encanada esteve relacionada à distância do ponto de coleta com relação à estação de tratamento. Defeitos na canalização aumentaram o índice de contaminação (p<0,5, principalmente em áreas densamente povoadas com infra-estrutura arruinada. Os despejos de bueiros representaram a principal fonte de contaminação da água encanada. Houve maior contaminação em poços superficiais do que

  10. Latent class analysis of the diagnostic characteristics of PCR and conventional bacteriological culture in diagnosing intramammary infections caused by Staphylococcus aureus in dairy cows at dry off

    Directory of Open Access Journals (Sweden)

    Cederlöf Sara Ellinor

    2012-11-01

    Full Text Available Abstract Background Staphylococcus aureus is one of the most common causes of intramammary infections in dairy cows at dry off. Reliable identification is important for disease management on herd level and for antimicrobial treatment of infected animals. Our objective was to evaluate the test characteristics of PathoProof ™ Mastitis PCR Assay and bacteriological culture (BC in diagnosing bovine intramammary infections caused by S. aureus at dry off at different PCR cycle threshold (Ct-value cut-offs. Methods Sterile quarter samples and non-sterile composite samples from 140 animals in seven herds were collected in connection with the dairy herd improvement (DHI milk recording. All quarter samples were analyzed using BC whereas all composite samples were analyzed with PathoProof ™ Mastitis PCR Assay. Latent class analysis was used to estimate test properties for PCR and BC in the absence of a perfect reference test. The population was divided into two geographically divided subpopulations and the Hui-Walter 2-test 2-populations model applied to estimate Se, Sp for the two tests, and prevalence for the two subpopulations. Results The Se for PCR increased with increasing Ct-value cut-off, accompanied by a small decrease in Sp. For BC the Se decreased and Sp increased with increasing Ct-value cut-off. Most optimal test estimates for the real-time PCR assay were at a Ct-value cut-off of 37; 0.93 [95% posterior probability interval (PPI 0.60-0.99] for Se and 0.95 [95% PPI 0.95-0.99] for Sp. At the same Ct-value cut-off, Se and Sp for BC were 0.83 [95% PPI 0.66-0.99] and 0.97 [95% PPI 0.91-0.99] respectively. Depending on the chosen PCR Ct-value cut-off, the prevalence in the subpopulations varied; the prevalence increased with increasing PCR Ct-value cut-offs. Conclusion Neither BC nor real-time PCR is a perfect test in detecting IMI in dairy cows at dry off. The changes in sensitivity and prevalence at different Ct-value cut-offs for both PCR and

  11. Clinical and bacteriological efficacy of twice daily topical retapamulin ointment 1% in the management of impetigo and other uncomplicated superficial skin infections.

    Science.gov (United States)

    Bohaty, Benjamin R; Choi, Sangbum; Cai, Chunyan; Hebert, Adelaide A

    2015-02-01

    Cutaneous bacterial infections are common in children and adults and frequently are caused by Staphylococcus aureus ( S. aureus ). Treatment failures with topical agents are not uncommon and have been shown to be secondary to bacterial resistance. To determine clinical and bacteriological efficacy of retapamulin ointment 1% in treatment of patients with cutaneous bacterial infections caused by methicillin-resistant S. aureus (MRSA) and other bacteria. Prospective, nonrandomized, uncontrolled, open label, single center trial conducted between April 2008 and November 2012 that evaluated efficacy of retapamulin ointment 1% in the treatment of impetigo, folliculitis, and other minor soft tissue infections in children and adults. Fifty patients, who presented to a dermatology outpatient clinic and were clinically diagnosed with impetigo, folliculitis, or minor soft tissue infection suitable for treatment with a topical antibiotic, were screened. Thirty-eight patients were enrolled and received treatment: topical retapamulin ointment 1% twice daily for 5 days. Seven patients were MRSA positive and qualified for the primary efficacy population. One patient withdrew due to an adverse event. Clinical and microbiological exams were performed at baseline and follow-up 5 to 7 days later to assess clinical, microbiological, and therapeutic responses. Primary outcome was clinical response at follow-up in primary efficacy population with MRSA isolated as the baseline pathogen. Secondary outcomes included clinical, microbiologic, and therapeutic responses in patients who were culture positive for any species of bacteria. Clinical response at follow-up in the primary efficacy population (MRSA-positive patients) was not sufficiently powered to demonstrate significance; however, outcomes were excellent, with 7 of 7 patients demonstrating clinical success (5 of 7) or clinical improvement (2 of 7) at follow-up. Barring lack of significance due to small total sample size for patients

  12. Análise bacteriológica da água de equipos odontológicos Bacteriological analysis of water in dental units

    Directory of Open Access Journals (Sweden)

    Maria Cristina Bronharo Tognim

    2000-05-01

    Full Text Available Nos últimos anos, grande esforço tem sido feito para conscientizar os profissionais da saúde sobre a importância dos processos de esterilização e anti-sepsia. Entretanto, pouca atenção tem sido dispensada pelos cirurgiões-dentistas à qualidade da água utilizada na prática odontológica. Neste trabalho, realizamos 72 análises bacteriológicas em amostras de água de 68 equipos odontológicos e de 4 torneiras, para verificar a qualidade microbiológica da água, através da contagem de bactérias heterotróficas e pesquisa de coliformes. As amostras coletadas das torneiras apresentaram valores compatíveis com os padrões de potabilidade. Das 68 amostras obtidas dos equipos 63 (92,7% estavam contaminadas e 5 (7,3% estavam dentro dos padrões de potabilidade. Observou-se que 42 equipos (61,8% continham água acentuadamente contaminada, 14 (20,6% apresentaram índice moderado de contaminação e 7 (10,3% estavam com a água levemente contaminada. Estes resultados apontam a necessidade de cuidados especiais, tais como a descontaminação e a desinfecção periódica dos sistemas de água dos equiposAlthough, in recent years, great efforts have been made to inform and make health professionals aware of the importance of sterilization, disinfection and antisepsis, little considerations have been shown in relation to the quality of the water of dental unit systems. In the present work 72 samples of water from 68 dental equipments and 4 faucets were submitted to bacteriological analysis, with the objective of detecting heterothrophic bacteria and coliform group. The samples collected from the faucets were compatible with the water potability patterns. In contrast, 63 (92.7% of the 68 samples obtained from the dental unit water systems were considered contaminated and only 5 (7.3% were in agreement with the potability patterns. It was observed that 42 water samples (61.8% were heavily contaminated, 14 (20.6% presented moderate index of

  13. Alternative anaerobic enrichments to the bacteriological analytical manual culture method for isolation of Shigella sonnei from selected types of fresh produce.

    Science.gov (United States)

    Jacobson, Andrew P; Thunberg, Richard L; Johnson, Mildred L; Hammack, Thomas S; Andrews, Wallace H

    2004-01-01

    Alternative methods of reducing oxygen during anaerobic enrichment in the Bacteriological Analytical Manual (BAM) Shigella culture method were evaluated and compared to the current and less practical GasPak method. The alternative anaerobic methods included the use of reducing agents in Shigella broth and reducing culture container headspace volume to minimize atmospheric effects on oxygen concentration in Shigella broth during enrichment. The reducing agents evaluated were sodium thioglycollate, L-cystine, L-cysteine, titanium(III) citrate, and dithiothreitol, each at concentrations of 0.1, 0.05, and 0.01%. The use of Oxyrase for Broth with the enrichment medium (Shigella broth) was evaluated at concentrations of 10, 20 and 30 microL/mL. Recoveries of chill- and freeze-stressed S. sonnei strains 357 and 20143 were determined with each anaerobic method, including the GasPak method, using inoculation levels ranging from 10(0)to 10(3) cells. For each anaerobic method, strain, inoculation level, and stress type, 5 replicate enrichments were evaluated by streaking to MacConkey agar for isolation. The numbers of cultures with each method from which S. sonnei was isolated were used to compare the alternative anaerobic methods to the GasPak method. The alternative anaerobic method with which chill- and freeze-stressed S. sonnei strains 357 and 20143 were isolated most consistently was the use of Oxyrase for Broth in Shigella broth at a concentration of 20 microL/mL. This method was compared to the GasPak anaerobic method in evaluations on the recovery of S. sonnei strains 357 and 20143 from artificially contaminated test portions of parsley, cilantro, green onions, strawberries, carrots, and celery. A third anaerobic method included the use of 0.5 cm mineral oil overlay on cultures containing Oxyrase for Broth at concentrations of 20 microL/mL. Recovery rates of strain 357 were significantly greater (p cultures. The use of mineral oil overlay with Oxyrase for Broth also

  14. BACTERIOLOGICAL QUALITY OF TAP WATER

    Directory of Open Access Journals (Sweden)

    Justyna Zamorska

    2016-06-01

    Full Text Available The most sensitive method of detecting contamination in water supply networks is microbiological testing. Microbiological water safety is evaluated mainly based on the results of traditional tests that rely on bacteria culturing on the so called bacterial growth mediums. Flow cytometry is a modern technology that has been used in microbiology only recently. The diagnostic method based on flow cytometry is much faster and more versatile. Microbiological quality testing was conducted in rzeszowski district, in the area of water network supplied by surface waters, and in the area of water network supplied by underground waters. The scope of the analysis of the microbiological quality of tap water was based on the determination of selected indicators of the sanitary condition of water ie; the total number of psychrophilic and mesophilic bacteria on nutrient agar (reference called Agar A and additionally called agar supplemented with R, the number of coliforms and faecal streptococci. Determination of the total number of microorganisms by flow cytometry was performed using two dyes SYBR Green and iodide pyridine. Water from underground water intakes, not under the permanent control of microbial had worse microbiological parameters. Used new methods of microbiological assays showed greater amounts of microbiological contamination.

  15. the bacteriological safety and antimicrobial

    African Journals Online (AJOL)

    GB

    2013-03-01

    Mar 1, 2013 ... serotypes Typhimurium, Enteritidis and. Infantis from human infections, food stuffs and farm animals in Italy. Epidemiol Infec,. 2004; 132: 245-251. 34. Centinkaya F, Cibik G, Soyuteniz E, Ozkin C,. Kayali R, Levent B. Shigella and Salmonella contamination in various foodstuffs in Turkey. Food Control, 2008 ...

  16. BACTERIOLOGICAL ANALYSIS AND PUBLIC PERCEPTION ...

    African Journals Online (AJOL)

    Hamaidi-Chergui F, Brahim Errahmani M, Debib A, Hamaidi M.S

    2016-05-01

    . These results ... be free from organisms and chemical substances in concentration large enough to affect health. [8]. In Algeria, the two ... Location of Arib area in Ain Defla (Algeria) (Agence nationale de Développement de.

  17. BACTERIOLOGICAL AND PARASITOLOGICAL ASSESSMENT OF ...

    African Journals Online (AJOL)

    Samples were collected from subject in lithotomy position using sterile cuscos bivalve speculum. Samples were ... Since vaginitis could be asymptomatic most time, the screening of all pregnant women with risk factors for preterm labour and premature rupture of membranes must be undertaken. Prompt treatment of cases is ...

  18. Bacteriology of diabetic foot lesions.

    Science.gov (United States)

    Yoga, R; Khairul, A; Sunita, K; Suresh, C

    2006-02-01

    Infection plays a pivotal role in enhancing a diabetic foot at risk toward amputation. Effective antibiotic therapy against the offending pathogens is an important component of treatment of diabetic foot infections. Recognition of the pathogen is always difficult as the representative deep tissue sample for culture is surrounded by ulcer surface harbouring colonies of organisms frequently labelled as skin commensals. The emergent of resistant strains represents a compounding problem standing against efforts to prevent amputation. This study was undertaken to identify the pathogens associated with diabetic foot infection in terms of their frequency and sensitivity against certain commonly used antibiotics. Forty-four consecutive patients with open diabetic foot infections had wound swab taken for culture and sensitivity testing. Cultures positive were observed in 89% of the cases with Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeroginosa encountered in 20%, 14% and 14% of cases respectively. Mixed growths were isolated in 6% of cultures. All Staphylcoccus aureus isolates were resistant to Penicillin but 80% were sensitive to Erythromycin and Co-trimoxazole. Klebsiella pneumoniae isolates were sensitive to Methicillin and Gentamycin in 80% and 60% of cases respectively, and resistant to Ampicillin and Ceftazidime in 83% and 50% respectively. All Pseudomonas aeroginosa isolates were sensitive to Amikacin and Ciprofloxacin but 50% were resistant to Gentamycin. There was no single antibiotic possessing good coverage for all common organisms isolated from diabetic foot lesions. Staphylococcus aureus remains the predominant cause of diabetic foot infections followed by Klebsiela pneumonia and Pseudomonas aeroginosa. Most infections are monomicrobial. The emergence of multiresistant organisms is a worrying feature in diabetic foot infections.

  19. Mobile Game for Learning Bacteriology

    Science.gov (United States)

    Sugimura, Ryo; Kawazu, Sotaro; Tamari, Hiroki; Watanabe, Kodai; Nishimura, Yohei; Oguma, Toshiki; Watanabe, Katsushiro; Kaneko, Kosuke; Okada, Yoshihiro; Yoshida, Motofumi; Takano, Shigeru; Inoue, Hitoshi

    2014-01-01

    This paper treats serious games. Recently, one of the game genres called serious game has become popular, which has other purposes besides enjoyments like education, training and so on. Especially, learning games of the serious games seem very attractive for the age of video games so that the authors developed a mobile game for learning…

  20. Bacteriological studies on Limburger cheese

    NARCIS (Netherlands)

    El-Erian, A.F.M.

    1969-01-01

    A study has been made of the ripening process of Limburger cheese, including microbiological investigations of the surface flora and chemical analyses of the cheese during the different stages of ripening. The microbial flora was studied both qualitatively and quantitatively. In addition to the

  1. bacteriological examination of chronic osteomyelitis

    African Journals Online (AJOL)

    Approximately 2 ml of venous blood was obtained from each ... Enterobacter aerogenes ATCC considerable proportion of. 13042 were run as control' infections. Decubitus ulcers organisms. contributed 5 cases. This was followed by diabetic foot with 4. RESULTS cases. Leg ulcer, pin—tract infection,. A total of 82 patients ...

  2. PHYSICOCHEMICAL AND BACTERIOLOGICAL ANALYSIS OF ...

    African Journals Online (AJOL)

    cornerstone

    pH, electrical conductivity, turbidity, dissolved oxygen (DO) and Biochemical Oxygen Demand. (BOD) were 5.98, 22.8μs/cm, 18.1NTU, ... beneath the earth's surface in soil pore spaces and in the fractures of rock .... Vol. 17, 2012. Table 1: Physical and chemical parameters of well water samples. SAMPLES. Parameter. 1. 2.

  3. BACTERIOLOGICAL QUALITY OF TAP WATER

    OpenAIRE

    Justyna Zamorska; Monika Zdeb; Dorota Papciak

    2016-01-01

    The most sensitive method of detecting contamination in water supply networks is microbiological testing. Microbiological water safety is evaluated mainly based on the results of traditional tests that rely on bacteria culturing on the so called bacterial growth mediums. Flow cytometry is a modern technology that has been used in microbiology only recently. The diagnostic method based on flow cytometry is much faster and more versatile. Microbiological quality testing was conducted in rzes...

  4. Controle bacteriológico de carne caprina para elaboração de hambúrguer caprino defumado Bacteriological control of goat meat to elaboration of smoked goat hamburger

    Directory of Open Access Journals (Sweden)

    J.C. Metri

    2006-06-01

    Full Text Available Realizaram-se avaliações bacteriológicas da matéria-prima utilizada na elaboração de um produto cárneo caprino tipo hambúrguer defumado, antes e após sanitização. A carne foi tratada por aspersão, com uma solução de ácidos orgânicos contendo 2% de ácido acético, 1% de ácido lático, 0,25% de ácido cítrico e 0,1% de ácido ascórbico, e armazenada sob refrigeração. As análises bacteriológicas foram realizadas no dia 0 e aos 7 e 14 dias após defumação. Na carne, no dia 0, a contagem de coliformes fecais estava acima do limite permitido pela legislação vigente, mas sete dias após a sanitização, esse parâmetro estava dentro dos limites permitidos. No produto final todos os parâmetros bacteriológicos encontraram-se dentro dos padrões higiênico-sanitários.In order to obtain a safe goat smoked hamburger, bacteriological analysis of the raw material was performed before and after sanitation. Meat was sprayed with a solution of organic acids containing 2% acetic acid, 1% lactic acid, 0.25% citric acid and 0.1% ascorbic acid and, after that, stored under refrigeration. Bacteriological analysis was carried out on days 0, 7 and 14 after goat smoked hamburger processing. In meat, on day 0, the fecal coliform count was above legal limits, but on day 7 after sanitation this parameter was these limits. In the final product, all the results of the bacteriological parameters were according to the sanitary-hygienic standards.

  5. Point of Technique

    African Journals Online (AJOL)

    .

    hotmail.com. Introduction. Minimally invasive techniques are becoming popular choice for the recent times. These techniques are lowering the cost and giving the best cosmetic results. For cardiovascular surgery these techniques are much more.

  6. Cogeneration techniques; Les techniques de cogeneration

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-10-01

    This dossier about cogeneration techniques comprises 12 parts dealing successively with: the advantages of cogeneration (examples of installations, electrical and thermal efficiency); the combustion turbine (principle, performances, types); the alternative internal combustion engines (principle, types, rotation speed, comparative performances); the different configurations of cogeneration installations based on alternative engines and based on steam turbines (coal, heavy fuel and natural gas-fueled turbines); the environmental constraints of combustion turbines (pollutants, techniques of reduction of pollutant emissions); the environmental constraints of alternative internal combustion engines (gas and diesel engines); cogeneration and energy saving; the techniques of reduction of pollutant emissions (pollutants, unburnt hydrocarbons, primary and secondary (catalytic) techniques, post-combustion); the most-advanced configurations of cogeneration installations for enhanced performances (counter-pressure turbines, massive steam injection cycles, turbo-chargers); comparison between the performances of the different cogeneration techniques; the tri-generation technique (compression and absorption cycles). (J.S.)

  7. Use of real-time PCR on faecal samples for detection of sub-clinical Salmonella infection in cattle did not improve the detection sensitivity compared to conventional bacteriology

    DEFF Research Database (Denmark)

    Jensen, Annette Nygaard; Nielsen, L.R.; Baggesen, Dorte Lau

    2013-01-01

    bacteriological culture-reference method (BCRM) on cattle faecal samples for detection of sub-clinical Salmonella infections in cattle. Thirty faecal samples were artificially contaminated with either 10 or 50CFU of one of five strains of S. Dublin (SD) and S. Typhimurium (ST). The overall detection sensitivity...... of both rt-PCR and BCRM was 100% for ST and 78% for SD. Furthermore, 163 faecal samples from cattle herds with suspected Salmonella infection were tested to compare the relative performance of rt-PCR to BCRM on samples from naturally infected herds. The relative sensitivity of rt-PCR was 20% (3/15 BCRM...... positive samples) while the relative specificity and accuracy was 99% and 92%, respectively. Both methods had limitations for detecting low levels of SD (...

  8. Training techniques for industry

    International Nuclear Information System (INIS)

    Jones, D.W.

    1978-01-01

    The advantages and disadvantages of commonly used training techniques in relation to cost-effective, prevention-oriented Quality Assurance are examined. Important questions are whether training techniques teach cost effectiveness and whether the techniques are, themselves, cost effective. To answer these questions, criteria for evaluating teaching techniques for cost effectiveness were developd, and then commonly used techniques are evaluated in terms of specific training program objectives. Motivation of personnel is also considered important to the success of a training program, and methods are outlined by which recognition of the academic quality of industrial training can be used as a motivational technique

  9. Surface science techniques

    CERN Document Server

    Bracco, Gianangelo

    2013-01-01

    The book describes the experimental techniques employed to study surfaces and interfaces. The emphasis is on the experimental method. Therefore all chapters start with an introduction of the scientific problem, the theory necessary to understand how the technique works and how to understand the results. Descriptions of real experimental setups, experimental results at different systems are given to show both the strength and the limits of the technique. In a final part the new developments and possible extensions of the techniques are presented. The included techniques provide microscopic as well as macroscopic information. They cover most of the techniques used in surface science.

  10. Airway Clearance Techniques (ACTs)

    Medline Plus

    Full Text Available ... of treatment options. Airway Clearance Active Cycle of Breathing Technique Airway Clearance Techniques Autogenic Drainage Basics of ... Pulmonary Exacerbations Clinical Care Guidelines SCREENING & TREATING DEPRESSION & ANXIETY GUIDELINES Clinician Resources As a clinician, you’re ...

  11. Airway Clearance Techniques (ACTs)

    Medline Plus

    Full Text Available ... CYSTIC FIBROSIS Learn about cystic fibrosis, a genetic lung disorder that affects the pancreas and other organs, ... Technique Airway Clearance Techniques Autogenic Drainage Basics of Lung Care Chest Physical Therapy Coughing and Huffing High- ...

  12. Airway Clearance Techniques (ACTs)

    Medline Plus

    Full Text Available ... Cycle of Breathing Technique Airway Clearance Techniques Autogenic Drainage Basics of Lung Care Chest Physical Therapy Coughing ... Facebook Twitter Email More Related Content Medications Autogenic Drainage Positive Expiratory Pressure High-Frequency Chest Wall Oscillation ( ...

  13. Airway Clearance Techniques (ACTs)

    Medline Plus

    Full Text Available ... Airway Clearance Airway Clearance Techniques (ACTs) There are different ways to clear your airways. Most are easy ... to loosen mucus from airway walls. See how different airway clearance techniques work to help you clear ...

  14. Airway Clearance Techniques (ACTs)

    Medline Plus

    Full Text Available ... CF Treatments and Therapies Airway Clearance Airway Clearance Techniques (ACTs) There are different ways to clear your ... for fitting ACTs into daily life Airway Clearance Techniques | Webcast To learn more about how you can ...

  15. Nuclear techniques in industry

    International Nuclear Information System (INIS)

    Hammad, F.H.

    1994-01-01

    Nuclear techniques are utilized in almost every industry. The discussion in this paper includes discussions on tracer methods and uses nucleonic control systems technology; non-destructive testing techniques and radiation technology. 1 fig., 2 tabs

  16. Airway Clearance Techniques (ACTs)

    Medline Plus

    Full Text Available ... many challenges, including medical, social, and financial. By learning more about how you can manage your disease every day, you can ultimately help find a ... Cycle of Breathing Technique Airway Clearance Techniques Autogenic ...

  17. Variational Monte Carlo Technique

    Indian Academy of Sciences (India)

    ias

    RESONANCE ⎜ August 2014. GENERAL ⎜ ARTICLE. Variational Monte Carlo Technique. Ground State Energies of Quantum Mechanical Systems. Sukanta Deb. Keywords. Variational methods, Monte. Carlo techniques, harmonic os- cillators, quantum mechanical systems. Sukanta Deb is an. Assistant Professor in the.

  18. Introduction to perturbation techniques

    CERN Document Server

    Nayfeh, Ali H

    2011-01-01

    Similarities, differences, advantages and limitations of perturbation techniques are pointed out concisely. The techniques are described by means of examples that consist mainly of algebraic and ordinary differential equations. Each chapter contains a number of exercises.

  19. Semiconductor Research Experimental Techniques

    CERN Document Server

    Balkan, Naci

    2012-01-01

    The book describes the fundamentals, latest developments and use of key experimental techniques for semiconductor research. It explains the application potential of various analytical methods and discusses the opportunities to apply particular analytical techniques to study novel semiconductor compounds, such as dilute nitride alloys. The emphasis is on the technique rather than on the particular system studied.

  20. Techniques in Teaching Deviance

    Science.gov (United States)

    Cheatwood, Derral; And Others

    1978-01-01

    Discusses two techniques for use on the college level in teaching about the sociology of deviance. The first technique helps students understand the relativity of their personal beliefs. The second technique deals with strategies for investigating membership in deviant social groups. (Author/DB)

  1. Point of Technique

    African Journals Online (AJOL)

    .

    Minimally invasive techniques are becoming popular choice for the recent times. These techniques are lowering the cost and giving the best cosmetic results. For cardiovascular surgery these techniques are much more newer and much more unknown. Open lumbar sympathectomy for certain indications is a very well ...

  2. Techniques for characterizing lignin

    Science.gov (United States)

    Nicole M. Stark; Daniel J. Yelle; Umesh P. Agarwal

    2016-01-01

    Many techniques are available to characterize lignin. The techniques presented in this chapter are considered nondegradative, which are commonly applied to lignin. A brief discussion of lignin structure is included with this chapter to aid the reader in understanding why the discussed characterization techniques are appropriate for the study of lignin. Because the...

  3. Nuclear techniques in hydrology

    International Nuclear Information System (INIS)

    Moser, H.

    1976-01-01

    The nuclear techniques used in hydrology are usually tracer techniques based on the use of nuclides either intentionally introduced into, or naturally present in the water. The low concentrations of these nuclides, which must be detected in groundwater and surface water, require special measurement techniques for the concentrations of radioactive or of stable nuclides. The nuclear techniques can be used most fruitfully in conjunction with conventional methods for the solution of problems in the areas of hydrology, hydrogeology and glacier hydrology. Nuclear techniques are used in practice in the areas of prospecting for water, environment protection and engineering hydrogeology. (orig.) [de

  4. Low current beam techniques

    Energy Technology Data Exchange (ETDEWEB)

    Saint, A.; Laird, J.S.; Bardos, R.A.; Legge, G.J.F. [Melbourne Univ., Parkville, VIC (Australia). School of Physics; Nishijima, T.; Sekiguchi, H. [Electrotechnical Laboratory, Tsukuba (Japan).

    1993-12-31

    Since the development of Scanning Transmission Microscopy (STIM) imaging in 1983 many low current beam techniques have been developed for the scanning (ion) microprobe. These include STIM tomography, Ion Beam Induced Current, Ion Beam Micromachining and Microlithography and Ionoluminense. Most of these techniques utilise beam currents of 10{sup -15} A down to single ions controlled by beam switching techniques This paper will discuss some of the low beam current techniques mentioned above, and indicate, some of their recent applications at MARC. A new STIM technique will be introduced that can be used to obtain Z-contrast with STIM resolution. 4 refs., 3 figs.

  5. Pulse holographic measurement techniques

    International Nuclear Information System (INIS)

    Kim, Cheol Jung; Baik, Seong Hoon; Hong, Seok Kyung; Kim, Jeong Moog; Kim, Duk Hyun

    1992-01-01

    With the development of laser, remote inspection techniques using laser have been growing on. The inspection and measurement techniques by pulse holography are well-established technique for precise measurement, and widely used in various fields of industry now. In nuclear industry, this technology is practically used because holographic inspection is remote, noncontact, and precise measurement technique. In relation to remote inspection technology in nuclear industry, state-of-the art of pulse HNDT (Holographic non-destructive testing) and holographic measurement techniques are examined. First of all, the fundamental principles as well as practical problems for applications are briefly described. The fields of pulse holography have been divided into the HNDT, flow visualization and distribution study, and other application techniques. Additionally holographic particle study, bubble chamber holography, and applications to other visualization techniques are described. Lastly, the current status for the researches and applications of pulse holography to nuclear industry which are carried out actively in Europe and USA, is described. (Author)

  6. Semiconductor Modeling Techniques

    CERN Document Server

    Xavier, Marie

    2012-01-01

    This book describes the key theoretical techniques for semiconductor research to quantitatively calculate and simulate the properties. It presents particular techniques to study novel semiconductor materials, such as 2D heterostructures, quantum wires, quantum dots and nitrogen containing III-V alloys. The book is aimed primarily at newcomers working in the field of semiconductor physics to give guidance in theory and experiment. The theoretical techniques for electronic and optoelectronic devices are explained in detail.

  7. Materials characterization techniques

    National Research Council Canada - National Science Library

    Zhang, Sam; Li, L; Kumar, Ashok

    2009-01-01

    "With an emphasis on practical applications and real-world case studies, Materials Characterization Techniques presents the principles of widely used advanced surface and structural characterization...

  8. Persian fencing techniques

    Directory of Open Access Journals (Sweden)

    Manouchehr Moshtagh Khorasani

    2012-07-01

    Full Text Available There are numerous manuscripts, poems and stories that describe, specifically and in detail, the different techniques used in Persian swordsmanship. The present article explains the origins and the techniques of Persian swordsmanship. The article also describes the traditional code of conduct for Persian warriors. Additionally, it describes an array of techniques that were deployed in actual combat in Iran’s history. Some of these techniques are represented via the miniatures that are reproduced herein. This is the first article on Persian swordsmanship published in any periodical.

  9. Uranium exploration techniques

    International Nuclear Information System (INIS)

    Nichols, C.E.

    1984-01-01

    The subject is discussed under the headings: introduction (genetic description of some uranium deposits; typical concentrations of uranium in the natural environment); sedimentary host rocks (sandstones; tabular deposits; roll-front deposits; black shales); metamorphic host rocks (exploration techniques); geologic techniques (alteration features in sandstones; favourable features in metamorphic rocks); geophysical techniques (radiometric surveys; surface vehicle methods; airborne methods; input surveys); geochemical techniques (hydrogeochemistry; petrogeochemistry; stream sediment geochemistry; pedogeochemistry; emanometry; biogeochemistry); geochemical model for roll-front deposits; geologic model for vein-like deposits. (U.K.)

  10. Bacteriological Profile and Antimicrobial Susceptibility Patterns of Bacteria Isolated from Pus/Wound Swab Samples from Children Attending a Tertiary Care Hospital in Kathmandu, Nepal

    Directory of Open Access Journals (Sweden)

    Salu Rai

    2017-01-01

    Full Text Available In Nepal, little is known about the microbiological profile of wound infections in children and their antimicrobial susceptibility patterns. Total of 450 pus/wound swab samples collected were cultured using standard microbiological techniques and the colonies grown were identified with the help of biochemical tests. The antimicrobial susceptibility testing was performed by Kirby-Bauer disc diffusion technique. Methicillin-resistant Staphylococcus aureus isolates were detected by using cefoxitin disc and confirmed by determining minimum inhibitory concentrations (MIC of oxacillin. 264 (59% samples were culture positive. The highest incidence of bacterial infections was noted in the age group of less than 1 year (76%. Out of 264 growth positive samples, Gram-positive bacteria were isolated from 162 (61% samples and Gram-negative bacteria were found in 102 (39% samples. Staphylococcus aureus (99% was the predominant Gram-positive bacteria isolated and Pseudomonas aeruginosa (44% was predominant Gram-negative bacteria. About 19% of S. aureus isolates were found to be methicillin-resistant MIC of oxacillin ranging from 4 μg/mL to 128 μg/mL. Among the children of Nepal, those of age less than 1 year were at higher risk of wound infections by bacteria. Staphylococcus aureus followed by Pseudomonas aeruginosa were the most common bacteria causing wound infections in children.

  11. Laser induced pyrolysis techniques

    International Nuclear Information System (INIS)

    Vanderborgh, N.E.

    1976-01-01

    The application of laser pyrolysis techniques to the problems of chemical analysis is discussed. The processes occurring during laser pyrolysis are first briefly reviewed. The problems encountered in laser pyrolysis gas chromatography are discussed using the analysis of phenanthrene and binary hydrocarbons. The application of this technique to the characterization of naturally occurring carbonaceous material such as oil shales and coal is illustrated

  12. Emerging optical nanoscopy techniques

    Science.gov (United States)

    Montgomery, Paul C; Leong-Hoi, Audrey

    2015-01-01

    To face the challenges of modern health care, new imaging techniques with subcellular resolution or detection over wide fields are required. Far field optical nanoscopy presents many new solutions, providing high resolution or detection at high speed. We present a new classification scheme to help appreciate the growing number of optical nanoscopy techniques. We underline an important distinction between superresolution techniques that provide improved resolving power and nanodetection techniques for characterizing unresolved nanostructures. Some of the emerging techniques within these two categories are highlighted with applications in biophysics and medicine. Recent techniques employing wider angle imaging by digital holography and scattering lens microscopy allow superresolution to be achieved for subcellular and even in vivo, imaging without labeling. Nanodetection techniques are divided into four subcategories using contrast, phase, deconvolution, and nanomarkers. Contrast enhancement is illustrated by means of a polarized light-based technique and with strobed phase-contrast microscopy to reveal nanostructures. Very high sensitivity phase measurement using interference microscopy is shown to provide nanometric surface roughness measurement or to reveal internal nanometric structures. Finally, the use of nanomarkers is illustrated with stochastic fluorescence microscopy for mapping intracellular structures. We also present some of the future perspectives of optical nanoscopy. PMID:26491270

  13. A Technique: Exposure Therapy

    Directory of Open Access Journals (Sweden)

    Serkan AKKOYUNLU

    2013-08-01

    Conclusion: Exposure with response prevention is a basic and effective technique. Every cognitive behavior therapist must be able to implement this technique and be cognizant of pearls of this procedure. (Journal of Cognitive Behavioral Psychotherapy and Research 2013, 2: 121-128 [JCBPR 2013; 2(2.000: 121-128

  14. Analog signal isolation techniques

    Energy Technology Data Exchange (ETDEWEB)

    Beadle, E.R.

    1992-12-31

    This paper discusses several techniques for isolating analog signals in an accelerator environment. The techniques presented here encompass isolation amplifiers, voltage-to-frequency converters (VIFCs), transformers, optocouplers, discrete fiber optics, and commercial fiber optic links. Included within the presentation of each method are the design issues that must be considered when selecting the isolation method for a specific application.

  15. Analog signal isolation techniques

    Energy Technology Data Exchange (ETDEWEB)

    Beadle, E.R.

    1992-01-01

    This paper discusses several techniques for isolating analog signals in an accelerator environment. The techniques presented here encompass isolation amplifiers, voltage-to-frequency converters (VIFCs), transformers, optocouplers, discrete fiber optics, and commercial fiber optic links. Included within the presentation of each method are the design issues that must be considered when selecting the isolation method for a specific application.

  16. Contamination Control Techniques

    International Nuclear Information System (INIS)

    EBY, J.L.

    2000-01-01

    Welcome to a workshop on contamination Control techniques. This work shop is designed for about two hours. Attendee participation is encouraged during the workshop. We will address different topics within contamination control techniques; present processes, products and equipment used here at Hanford and then open the floor to you, the attendees for your input on the topics

  17. Microscopic techniques in biotechnology

    National Research Council Canada - National Science Library

    Hoppert, M

    2003-01-01

    ... techniques. Over the past 50 years, electron microscopy has opened new pathways for biologists to understand structure-function interrelations at the subcellular level. New lightbased optical techniques involving the detection of fluorescent molecules have introduced a new dimension of imaging into light microscopy, enabling cellular events to be studied in vivo d...

  18. Contamination Control Techniques

    Energy Technology Data Exchange (ETDEWEB)

    EBY, J.L.

    2000-05-16

    Welcome to a workshop on contamination Control techniques. This work shop is designed for about two hours. Attendee participation is encouraged during the workshop. We will address different topics within contamination control techniques; present processes, products and equipment used here at Hanford and then open the floor to you, the attendees for your input on the topics.

  19. Glycoprotein and proteoglycan techniques

    International Nuclear Information System (INIS)

    Beeley, J.G.

    1985-01-01

    The aim of this book is to describe techniques which can be used to answer some of the basic questions about glycosylated proteins. Methods are discussed for isolation, compositional analysis, and for determination of the primary structure of carbohydrate units and the nature of protein-carbohydrate linkages of glycoproteins and proteoglycans. High resolution NMR is considered, as well as radioactive labelling techniques. (Auth.)

  20. Emerging optical nanoscopy techniques

    Directory of Open Access Journals (Sweden)

    Montgomery PC

    2015-09-01

    Full Text Available Paul C Montgomery, Audrey Leong-Hoi Laboratoire des Sciences de l'Ingénieur, de l'Informatique et de l'Imagerie (ICube, Unistra-CNRS, Strasbourg, France Abstract: To face the challenges of modern health care, new imaging techniques with subcellular resolution or detection over wide fields are required. Far field optical nanoscopy presents many new solutions, providing high resolution or detection at high speed. We present a new classification scheme to help appreciate the growing number of optical nanoscopy techniques. We underline an important distinction between superresolution techniques that provide improved resolving power and nanodetection techniques for characterizing unresolved nanostructures. Some of the emerging techniques within these two categories are highlighted with applications in biophysics and medicine. Recent techniques employing wider angle imaging by digital holography and scattering lens microscopy allow superresolution to be achieved for subcellular and even in vivo, imaging without labeling. Nanodetection techniques are divided into four subcategories using contrast, phase, deconvolution, and nanomarkers. Contrast enhancement is illustrated by means of a polarized light-based technique and with strobed phase-contrast microscopy to reveal nanostructures. Very high sensitivity phase measurement using interference microscopy is shown to provide nanometric surface roughness measurement or to reveal internal nanometric structures. Finally, the use of nanomarkers is illustrated with stochastic fluorescence microscopy for mapping intracellular structures. We also present some of the future perspectives of optical nanoscopy. Keywords: microscopy, imaging, superresolution, nanodetection, biophysics, medical imaging

  1. [New microbiological techniques].

    Science.gov (United States)

    Schubert, S; Wieser, A; Bonkat, G

    2017-06-01

    Microbiological diagnostic procedures have changed rapidly in recent years. This is especially true in the field of molecular diagnostics. Classical culture-based techniques are still the gold standard in many areas; however, they are already complemented by automated and also molecular techniques to guarantee faster and better quality results. The most commonly used techniques include real-time polymerase chain reaction (RT-PCR) based systems and nucleic acid hybridization. These procedures are used most powerfully from direct patient samples or in assays to detect the presence of nonculturable or fastidious organisms. Further techniques such as DNA sequencing are not yet used routinely for urological samples and can be considered experimental. However, in conjunction with dropping prices and further technical developments, these techniques promise to be used much more in the near future. Regarding bacterial identification from culture, mass spectrometry (MALDI-TOF MS) has become the technique of choice in recent years especially in Europe. It has tremendously shortened the time to result. This is now going to be extended to antibiotic susceptibility testing. This is of paramount importance in view of ever rising antimicrobial resistance rates. Techniques described in this review offer a faster and better microbiological diagnosis. Such continuous improvements are critical especially in times of cost pressure and rising antimicrobial resistance rates. It is in our interest to provide the best possible care for patients and in this regard a good and effective communication between the laboratory and the clinician is of vital importance.

  2. Computer techniques for electromagnetics

    CERN Document Server

    Mittra, R

    1973-01-01

    Computer Techniques for Electromagnetics discusses the ways in which computer techniques solve practical problems in electromagnetics. It discusses the impact of the emergence of high-speed computers in the study of electromagnetics. This text provides a brief background on the approaches used by mathematical analysts in solving integral equations. It also demonstrates how to use computer techniques in computing current distribution, radar scattering, and waveguide discontinuities, and inverse scattering. This book will be useful for students looking for a comprehensive text on computer techni

  3. Advanced analytical techniques

    International Nuclear Information System (INIS)

    Mrochek, J.E.; Shumate, S.E.; Genung, R.K.; Bahner, C.T.; Lee, N.E.; Dinsmore, S.R.

    1976-01-01

    The development of several new analytical techniques for use in clinical diagnosis and biomedical research is reported. These include: high-resolution liquid chromatographic systems for the early detection of pathological molecular constituents in physiologic body fluids; gradient elution chromatography for the analysis of protein-bound carbohydrates in blood serum samples, with emphasis on changes in sera from breast cancer patients; electrophoretic separation techniques coupled with staining of specific proteins in cellular isoenzymes for the monitoring of genetic mutations and abnormal molecular constituents in blood samples; and the development of a centrifugal elution chromatographic technique for the assay of specific proteins and immunoglobulins in human blood serum samples

  4. Spectrometric techniques 2

    CERN Document Server

    Vanasse, George A

    2013-01-01

    Spectrometric Techniques, Volume II provides information pertinent to vacuum ultraviolet techniques to complete the demonstration of the diversity of methods available to the spectroscopist interested in the ultraviolet visible and infrared spectral regions. This book discusses the specific aspects of the technique of Fourier transform spectroscopy.Organized into five chapters, this volume begins with an overview of the large number of systematic effects in the recording of an interferogram. This text then examines the design approach for a Fourier transform spectrometer with focus on optics.

  5. Spectrometric techniques 3

    CERN Document Server

    Vanasse, George A

    2013-01-01

    Spectrometric Techniques, Volume III presents the applications of spectrometric techniques to atmospheric and space studies. This book reviews the spectral data processing and analysis techniques that are of broad applicability.Organized into five chapters, this volume begins with an overview of the instrumentation used for obtaining field data. This text then reviews the contribution that space-borne spectroscopy in the thermal IR has made to the understanding of the planets. Other chapters consider the instruments that have recorded the planetary emission spectra. This book discusses as well

  6. Separation techniques: Chromatography

    Science.gov (United States)

    Coskun, Ozlem

    2016-01-01

    Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. Proteins can be purified based on characteristics such as size and shape, total charge, hydrophobic groups present on the surface, and binding capacity with the stationary phase. Four separation techniques based on molecular characteristics and interaction type use mechanisms of ion exchange, surface adsorption, partition, and size exclusion. Other chromatography techniques are based on the stationary bed, including column, thin layer, and paper chromatography. Column chromatography is one of the most common methods of protein purification. PMID:28058406

  7. Spectrometric techniques 4

    CERN Document Server

    Vanasse, George A

    2013-01-01

    Spectrometric Techniques, Volume IV discusses three widely diversified areas of spectrometric techniques. The book focuses on three spectrometric methods. Chapter 1 discusses the phenomenology and applications of Coherent Anti-Stokes Raman Spectroscopy (CARS), the most commonly used optical technique that exploit the Raman effect. The second chapter is concerned with diffraction gratings and mountings for the Vacuum Ultraviolet Spectral Region. Chapter 3 accounts the uses of mass spectrometry, detectors, types of spectrometers, and ion sources. Physicists and chemists will find the book a go

  8. Airway Clearance Techniques (ACTs)

    Medline Plus

    Full Text Available ... Techniques Autogenic Drainage Basics of Lung Care Chest Physical Therapy Coughing and Huffing High-Frequency Chest Wall Oscillation ... Children and Adults Clinical Care Guidelines Nutrition in Pediatrics Clinical Care Guidelines Pancreatic Enzymes Clinical Care Guidelines ...

  9. Airway Clearance Techniques (ACTs)

    Medline Plus

    Full Text Available ... Twitter YouTube Instagram Email DONATE Breadcrumb Navigation Home Life With CF Treatments and Therapies Airway Clearance Airway Clearance Techniques (ACTs) There are different ways to clear your airways. Most are easy to ...

  10. Airway Clearance Techniques (ACTs)

    Medline Plus

    Full Text Available ... a Family Parenting as an Adult With CF Treatments & Therapies People with cystic fibrosis are living longer ... to specialized CF care and a range of treatment options. Airway Clearance Active Cycle of Breathing Technique ...

  11. Materials characterization techniques

    National Research Council Canada - National Science Library

    Zhang, Sam; Li, L; Kumar, Ashok

    2009-01-01

    ... techniques for quality assurance, contamination control, and process improvement. The book reviews the most popular and powerful analysis and quality control tools, explaining the appropriate uses and related technical requirements...

  12. Airway Clearance Techniques (ACTs)

    Medline Plus

    Full Text Available ... another member of your CF care team can show you how to perform many different ACTs and ... of children with CF and a respiratory therapist talk about the different techniques they use for airway ...

  13. Airway Clearance Techniques (ACTs)

    Medline Plus

    Full Text Available ... Treatments and Therapies Airway Clearance Airway Clearance Techniques (ACTs) There are different ways to clear your airways. ... or caregiver. Older kids and adults can choose ACTs that they can do on their own. Share ...

  14. RFCM Techniques Chamber Facility

    Data.gov (United States)

    Federal Laboratory Consortium — FUNCTION: Provides the capability to develop radio-frequency countermeasure (RFCM) techniques in a controlled environment from 2.0 to 40.0 GHz. The configuration of...

  15. Biaxial compression test technique

    Science.gov (United States)

    Hansard, E. T.

    1975-01-01

    Fixture and technique have been developed for predicting behavior of stiffened skin panels under biaxial compressive loading. Tester can load test panel independently in longitudinal and transverse directions. Data can also be obtained in combined mode.

  16. Plant tissue culture techniques

    Directory of Open Access Journals (Sweden)

    Rolf Dieter Illg

    1991-01-01

    Full Text Available Plant cell and tissue culture in a simple fashion refers to techniques which utilize either single plant cells, groups of unorganized cells (callus or organized tissues or organs put in culture, under controlled sterile conditions.

  17. Small Scale Organic Techniques

    Science.gov (United States)

    Horak, V.; Crist, DeLanson R.

    1975-01-01

    Discusses the advantages of using small scale experimentation in the undergraduate organic chemistry laboratory. Describes small scale filtration techniques as an example of a semi-micro method applied to small quantities of material. (MLH)

  18. Airway Clearance Techniques (ACTs)

    Medline Plus

    Full Text Available ... Techniques Autogenic Drainage Basics of Lung Care Chest Physical Therapy Coughing and Huffing High-Frequency Chest Wall ... Care Guidelines Newborn Screening Clinical Care Guidelines Sweat Test Clinical Care Guidelines Infection Prevention and Control Care ...

  19. Lightweight Cryptographic Techniques

    National Research Council Canada - National Science Library

    Yuen, Horace

    2004-01-01

    The objective of this project was to develop new cryptographic techniques, and to modify the important existing ones, for applications to encryption and authentication in energy-constrained sensors...

  20. Variational Monte Carlo Technique

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 19; Issue 8. Variational Monte Carlo Technique: Ground State Energies of Quantum Mechanical Systems. Sukanta Deb. General Article Volume 19 Issue 8 August 2014 pp 713-739 ...

  1. Radon survey techniques

    International Nuclear Information System (INIS)

    Anon.

    1976-01-01

    The report reviews radon measurement surveys in soils and in water. Special applications, and advantages and limitations of the radon measurement techniques are considered. The working group also gives some directions for further research in this field

  2. Stargate: Energy Management Techniques

    OpenAIRE

    Vijay Raghunathan; Mani Srivastava; Trevor Pering; Roy Want

    2004-01-01

    This poster presents techniques for energy efficient operation of the Stargate wireless platform. In addition to conventional power management techniques such as dynamic voltage and scaling and processor shutdown, the Stargate features several mechanisms for energy efficient operation of the communication subsystem, such as support for hierarchical radios, Bluetooth based remote wakeup, mote based wakeup, etc. Finally, design optimizations including the use of power gating, and provision for ...

  3. Applying contemporary statistical techniques

    CERN Document Server

    Wilcox, Rand R

    2003-01-01

    Applying Contemporary Statistical Techniques explains why traditional statistical methods are often inadequate or outdated when applied to modern problems. Wilcox demonstrates how new and more powerful techniques address these problems far more effectively, making these modern robust methods understandable, practical, and easily accessible.* Assumes no previous training in statistics * Explains how and why modern statistical methods provide more accurate results than conventional methods* Covers the latest developments on multiple comparisons * Includes recent advanc

  4. Imaging Techniques in Conservation

    OpenAIRE

    Payne, Emma Marie

    2013-01-01

    New imaging techniques are increasingly being used within cultural heritage. This paper explores potential uses of such technologies within conservation and implications of their use on object preservation and accessibility. Study of their effects on objects is crucial because their employment is becoming irreplaceable; for example, polynomial texture mapping (PTM) has revealed previously undetectable surface features. In such cases, it is necessary to continue to use the technique to monitor...

  5. Optimization techniques in statistics

    CERN Document Server

    Rustagi, Jagdish S

    1994-01-01

    Statistics help guide us to optimal decisions under uncertainty. A large variety of statistical problems are essentially solutions to optimization problems. The mathematical techniques of optimization are fundamentalto statistical theory and practice. In this book, Jagdish Rustagi provides full-spectrum coverage of these methods, ranging from classical optimization and Lagrange multipliers, to numerical techniques using gradients or direct search, to linear, nonlinear, and dynamic programming using the Kuhn-Tucker conditions or the Pontryagin maximal principle. Variational methods and optimiza

  6. Assisted reproductive techniques.

    Science.gov (United States)

    Huang, Jack Yu Jen; Rosenwaks, Zev

    2014-01-01

    Assisted reproductive technologies (ART) encompass fertility treatments, which involve manipulations of both oocyte and sperm in vitro. This chapter provides a brief overview of ART, including indications for treatment, ovarian reserve testing, selection of controlled ovarian hyperstimulation (COH) protocols, laboratory techniques of ART including in vitro fertilization (IVF), and intracytoplasmic sperm injection (ICSI), embryo transfer techniques, and luteal phase support. This chapter also discusses potential complications of ART, namely ovarian hyperstimulation syndrome (OHSS) and multiple gestations, and the perinatal outcomes of ART.

  7. Nonmammographic breast imaging techniques.

    Science.gov (United States)

    Heywang-Köbrunner, S H

    1992-10-01

    Significant progress in early detection of malignancy has been achieved by the improvement of mammographic technique, the introduction of quality control, the demonstration of benefits from screening, and appropriate application of supplementary methods such as ultrasound, cytology, and stereotaxis. Certain problems in breast imaging, however, are still unsolved. These include early detection and exclusion of malignancy without microcalcifications in mammographically dense tissue (particularly in younger women), the still-limited accuracy of mammographic signs, and the management of diagnostic problems after surgery, radiation therapy, or silicone implants. Therefore, research is needed to further improve diagnostic capabilities. The research can be subdivided into different approaches: 1) further development of the mammographic technique (digital luminescence radiography); 2) evaluation of techniques that image other physical tissue properties (sonography, thermography, trans-illumination, CT, non-contrast-enhanced MR imaging, biomagnetism, biostereometry, and ductoscopy); 3) investigation of techniques that image metabolic changes (MR spectroscopy, positron-emission tomography) or metabolism-induced differences in perfusion or vascularity (Doppler ultrasound, contrast-enhanced MR imaging); and 4) development of techniques that attempt tissue diagnosis using monoclonal antibodies. Among these techniques, digital luminescence radiography and contrast-enhanced MR imaging are the most developed and the most promising. They are at the threshold of becoming clinically important. Doppler ultrasound could be useful for certain indications. Whereas MR spectroscopy, positron-emission tomography, the search for appropriate antibodies, and possibly transillumination, ductoscopy, and biomagnetism offer interesting new aspects for research, the value of CT, thermography, and biostereometry is not yet established.

  8. Estudos bacteriológicos e sorológicos de um surto de peste no Estado da Paraíba, Brasil Bacteriological and serological studies of a plague outbreak in the Paraíba state, Brazil

    Directory of Open Access Journals (Sweden)

    Alzira Maria Paiva Almeida

    1989-06-01

    Full Text Available Foram realizados estudos bacteriológicos e/ou sorológicos para diagnóstico da infecção pestosa, em material obtido de 452 pacientes (48 positivos, 1.938 roedores e outros pequenos mamíferos (75 positivos, 4.756 cães (141 positivos e 3.047 gatos (57 positivos, oriundos de 41 municípios localizados em toda a extensão da área paraibana do Planalto da Borborema. A infecção foi encontrada em 21 municípios. Foram isoladas 20 cepas de Yersinia pestis de amostras coletadas de três pacientes e 17 roedores. Estas cepas apresentam características bioquímicas, fatores de virulência, sensibilidade aos antibióticos e poder patogênico experimental semelhantes ao de cepas isoladas anteriormente. Pelos estudos realizados não foram observados, no surto de peste que eclodiu em setembro de 1986 na Paraíba, fatores diferentes dos observados nos outros focos do nordeste do Brasil.During a plague outbreak in the Borborema Plateu focus (Paraíba, bacteriological and serological studies were carryed out in material from 452 patients (48 positives, 1,938 rodents and other small mammals (75 positives, 4,756 dogs (141 positives and 2,047 cats (57 positives obtained from 41 counties (out of which, 21 produced positive samples. Twenty Yersinia pestis strains isolated from material from 3 patients ans 17 rodents, displayed biochemical reactions, virulence factors, antibiotic susceptibility and animal experimental pathogenicity similar to those observed in strains previously isolated. According to our findings this recent plague outbreak did not exhibite different factors from those observed during prior outbreaks in other plague foci in the northeast of Brazil.

  9. Handbook of laboratory techniques

    International Nuclear Information System (INIS)

    2002-01-01

    The Nuclear Regulatory Authority in Argentina have laboratories of support to regulations functions on radiological and nuclear safety, safeguards and physical protection, that have a surface of 2950 m 2 in the Ezeiza Atomic Center. The manual describes in seven chapters the different techniques developed and applied in the laboratories along four decades of existence. The chapter 1: Dedicated to the treatment of environmental samples, described the procedures associated with the different types of samples: deposits, waters, sediments, vegetables, milk, fish and diet. The chapter 2: Details 48 radiochemical techniques associated to the measurements of americium 241, carbon 16, strontium 90, iodine 129, plutonium, radium 226, radon, uranium, nickel and actinides. The chapter 3: Describes the measurements techniques of alpha and gamma spectrometry. The different techniques of biological and physical dosimetry are described in the chapters 5 and 6 respectively. The final chapter is dedicated the techniques of external and internal contamination. It s important to emphasize that this manual contains the standardized technologies that the Nuclear Regulatory Authority of Argentina submits regularly to international comparisons

  10. Imaging Techniques in Conservation

    Directory of Open Access Journals (Sweden)

    Emma Marie Payne

    2013-02-01

    Full Text Available New imaging techniques are increasingly being used within cultural heritage. This paper explores potential uses of such technologies within conservation and implications of their use on object preservation and accessibility. Study of their effects on objects is crucial because their employment is becoming irreplaceable; for example, polynomial texture mapping (PTM has revealed previously undetectable surface features. In such cases, it is necessary to continue to use the technique to monitor object condition. 3D laser scanning, PTM, and CT scanning are investigated. Case studies are explored to investigate their current role in cultural heritage. The appropriateness of this role and whether it should be expanded is addressed by analysing advantages and disadvantages of the techniques, their feasibility, and risks caused to object preservation and accessibility. The results indicate that the technologies present some advantages over standard digital photography; PTM in particular is found to be an extremely useful, affordable technique. A more established role within conservation, especially for condition assessments, could be worthwhile. Use of the imaging techniques to create models for exhibition can also be advantageous; however, care must be taken to ensure that such models are used to enhance accessibility to original objects and not to replace them.

  11. Computer assisted audit techniques

    Directory of Open Access Journals (Sweden)

    Dražen Danić

    2008-12-01

    Full Text Available The purpose of this work is to point to the possibilities of more efficient auditing. In the encirclement of more and more intensive use of computer techniques that help to CAAT all the aims and the volume of auditing do not change when the audit is done in the computer-informatics environment. The computer assisted audit technique (CAATs can improve the efficiency and productivity of audit procedures. In the computerized information system, the CAATs are the ways in which an auditor can use computer to gather or as help in gathering auditing evidence. There are more reasons why the auditors apply computer techniques that help in auditing. Most often, they do it to achieve improvement of auditing efficiency when the data volume is large. It depends on several factors whether the auditors will apply the computer techniques that help auditing and to what degree respectively. If they do it, the most important are the computer knowledge, professional skill, experience of auditors, and availability of computer technique, and adequacy of computer supports, infeasibility of hand tests, efficiency and time limit. Through several examples from practice, we showed the possibilities of ACL as one of the CAAT tools.

  12. Relaxation Techniques for Trauma.

    Science.gov (United States)

    Scotland-Coogan, Diane; Davis, Erin

    2016-01-01

    Physiological symptoms of posttraumatic stress disorder (PTSD) manifest as increased arousal and reactivity seen as anger outburst, irritability, reckless behavior with no concern for consequences, hypervigilance, sleep disturbance, and problems with focus (American Psychiatric Association, 2013 ). In seeking the most beneficial treatment for PTSD, consideration must be given to the anxiety response. Relaxation techniques are shown to help address the physiological manifestations of prolonged stress. The techniques addressed by the authors in this article include mindfulness, deep breathing, yoga, and meditation. By utilizing these techniques traditional therapies can be complemented. In addition, those who are averse to the traditional evidence-based practices or for those who have tried traditional therapies without success; these alternative interventions may assist in lessening physiological manifestations of PTSD. Future research studies assessing the benefits of these treatment modalities are warranted to provide empirical evidence to support the efficacy of these treatments.

  13. Current techniques in mammography

    International Nuclear Information System (INIS)

    Bohm, B.

    1982-01-01

    This paper represents a literature survey of current techniques in mammography. The major objection to the otherwise acceptable methods in widespread use are the radiation doses given the patient, particularly the asymptomatic patient. Even using current techniques which involve x-raying the breast, all women over 50 should have yearly exams for the earliest possible cancer detection and hence improved diagnosis. It is for asymptomatic women under 50 that the risk versus benefit issue becomes controversial. Strong efforts are being made to reduce or eliminate this dose. Current methods in widespread clinical use and experimental methods are briefly covered, and the recommendations of the National Council on Radiation Protection and Measurements (released July 1980) are stressed. The current experimental techniques which include NMR, ultrasound, thermography, computer tomography, heavy particle radiography and ionography are capsulized

  14. Meniscal repair: Technique.

    Science.gov (United States)

    Beaufils, P; Pujol, N

    2018-02-01

    Meniscal repair aims to achieve meniscal healing, avoiding the adverse effects of meniscectomy. Longitudinal vertical tears in a vascularized area are the reference indication. The technique generally uses hybrid all-inside implants. The outside-in technique has other indications in more anterior tears. Healing has been demonstrated on CT-arthrography and arthroscopy. Specific techniques have been developed for other pathological situations. Posterior meniscosynovial lesions in a context of chronic anterior laxity are identified by exploration of the posterior compartment, and fixed by all-inside hook suture. Horizontal lesions in young athletes can be treated by open meniscal suture. Radial tears, when deep, can be repaired. Root tears, when traumatic, can be treated by transosseous pullout reinsertion. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  15. Telescopes and Techniques

    CERN Document Server

    Kitchin, C R

    2013-01-01

    Telescopes and Techniques has proved itself in its first two editions, having become probably one of the most widely used astronomy texts, both for amateur astronomers and astronomy and astrophysics undergraduates. Both earlier editions of the book were widely used for introductory practical astronomy courses in many universities. In this Third Edition the author guides the reader through the mathematics, physics and practical techniques needed to use today's telescopes (from the smaller models to the larger instruments installed in many colleges) and how to find objects in the sky. Most of the physics and engineering involved is described fully and requires little prior knowledge or experience. Both visual and electronic imaging techniques are covered, together with an introduction to how data (measurements) should be processed and analyzed. A simple introduction to radio telescopes is also included. Brief coverage of the more advanced topics of photometry and spectroscopy are included, but mainly to enable ...

  16. Radiological focusing techniques

    International Nuclear Information System (INIS)

    Husmann, K.; Mehrkens, A.; Hancken, G.

    1995-01-01

    The textbook explains the most common and essential radiological focusing techniques and gives the relevant theory and technical fundamentals. The theory does not consume much space in this book, the theoretical part briefly addressing such aspects as film-screen combinations, doses administered by X-ray radiography, quality assurance, radiological protection, and potential patient reactions to contrast media applied. The textbook primarily is a practical guide giving general advice and guidance on focusing techniques, accompanied by an illustrative compilation of frequent mistakes. Arranged by body regions, the well over 80 examples of focusing techniques are presented by systematic texts and illustrations. There is an annex giving a glossary of terms, a bibliography, and a list of useful addresses. (orig./MG) [de

  17. Novel food processing techniques

    Directory of Open Access Journals (Sweden)

    Vesna Lelas

    2006-12-01

    Full Text Available Recently, a lot of investigations have been focused on development of the novel mild food processing techniques with the aim to obtain the high quality food products. It is presumed also that they could substitute some of the traditional processes in the food industry. The investigations are primarily directed to usage of high hydrostatic pressure, ultrasound, tribomechanical micronization, microwaves, pulsed electrical fields. The results of the scientific researches refer to the fact that application of some of these processes in particular food industry can result in lots of benefits. A significant energy savings, shortening of process duration, mild thermal conditions, food products with better sensory characteristics and with higher nutritional values can be achieved. As some of these techniques act also on the molecular level changing the conformation, structure and electrical potential of organic as well as inorganic materials, the improvement of some functional properties of these components may occur. Common characteristics of all of these techniques are treatment at ambient or insignificant higher temperatures and short time of processing (1 to 10 minutes. High hydrostatic pressure applied to various foodstuffs can destroy some microorganisms, successfully modify molecule conformation and consequently improve functional properties of foods. At the same time it acts positively on the food products intend for freezing. Tribomechanical treatment causes micronization of various solid materials that results in nanoparticles and changes in structure and electrical potential of molecules. Therefore, the significant improvement of some rheological and functional properties of materials occurred. Ultrasound treatment proved to be potentially very successful technique of food processing. It can be used as a pretreatment to drying (decreases drying time and improves functional properties of food, as extraction process of various components

  18. Radiographic constant exposure technique

    DEFF Research Database (Denmark)

    Domanus, Joseph Czeslaw

    1985-01-01

    The constant exposure technique has been applied to assess various industrial radiographic systems. Different X-ray films and radiographic papers of two producers were compared. Special attention was given to fast film and paper used with fluorometallic screens. Radiographic image quality was tes...... was tested by the use of ISO wire IQI's and ASTM penetrameters used on Al and Fe test plates. Relative speed and reduction of kilovoltage obtained with the constant exposure technique were calculated. The advantages of fast radiographic systems are pointed out...

  19. Wireless communications algorithmic techniques

    CERN Document Server

    Vitetta, Giorgio; Colavolpe, Giulio; Pancaldi, Fabrizio; Martin, Philippa A

    2013-01-01

    This book introduces the theoretical elements at the basis of various classes of algorithms commonly employed in the physical layer (and, in part, in MAC layer) of wireless communications systems. It focuses on single user systems, so ignoring multiple access techniques. Moreover, emphasis is put on single-input single-output (SISO) systems, although some relevant topics about multiple-input multiple-output (MIMO) systems are also illustrated.Comprehensive wireless specific guide to algorithmic techniquesProvides a detailed analysis of channel equalization and channel coding for wi

  20. Modern recording techniques

    CERN Document Server

    Huber, David Miles

    2013-01-01

    As the most popular and authoritative guide to recording Modern Recording Techniques provides everything you need to master the tools and day to day practice of music recording and production. From room acoustics and running a session to mic placement and designing a studio Modern Recording Techniques will give you a really good grounding in the theory and industry practice. Expanded to include the latest digital audio technology the 7th edition now includes sections on podcasting, new surround sound formats and HD and audio.If you are just starting out or looking for a step up

  1. Radiographic constant exposure technique

    DEFF Research Database (Denmark)

    Domanus, Joseph Czeslaw

    1985-01-01

    The constant exposure technique has been applied to assess various industrial radiographic systems. Different X-ray films and radiographic papers of two producers were compared. Special attention was given to fast film and paper used with fluorometallic screens. Radiographic image quality...... was tested by the use of ISO wire IQI's and ASTM penetrameters used on Al and Fe test plates. Relative speed and reduction of kilovoltage obtained with the constant exposure technique were calculated. The advantages of fast radiographic systems are pointed out...

  2. Neutron visual sensing technique

    International Nuclear Information System (INIS)

    Kureta, Masatoshi; Segawa, Mariko

    2014-01-01

    The neutron visual sensing technique is a technology to extract physical quantities from the information on inner structures of complex materials or machineries which have been visualized and recorded by using neutron beams. Research and utilization of this technique is now under worldwide development since it can provide the information that is not possible by X-ray radiography. We show how to use stationary neutron sources (Research reactors) in chapter 2, and how to utilize pulsed neutron source (Japan Proton Accelerator Complex, J-PARC). Also the production of micro-element analyzer by an enterprise using the knowledge on radiological equipment is described as an example. (author)

  3. Millimicrosecond pulse techniques

    CERN Document Server

    Lewis, Ian A D

    1959-01-01

    Millimicrosecond Pulse Techniques, Second Edition focuses on millimicrosecond pulse techniques and the development of devices of large bandwidth, extending down to comparatively low frequencies (1 Mc/s). Emphasis is on basic circuit elements and pieces of equipment of universal application. Specific applications, mostly in the field of nuclear physics instrumentation, are considered. This book consists of eight chapters and opens with an introduction to some of the terminology employed by circuit engineers as well as theoretical concepts, including the laws of circuit analysis, Fourier analysi

  4. Single well techniques

    International Nuclear Information System (INIS)

    Drost, W.

    1983-01-01

    The single well technique method includes measurement of parameters of groundwater flow in saturated rock. For determination of filtration velocity the dilution of radioactive tracer is measured, for direction logging the collimeter is rotated in the probe linked with the compass. The limiting factor for measurement of high filtration velocities is the occurrence of turbulent flow. The single well technique is used in civil engineering projects, water works and subsurface drainage of liquid waste from disposal sites. The radioactive tracer method for logging the vertical fluid movement in bore-holes is broadly used in groundwater survey and exploitation. (author)

  5. Surface science techniques

    CERN Document Server

    Walls, JM

    2013-01-01

    This volume provides a comprehensive and up to the minute review of the techniques used to determine the nature and composition of surfaces. Originally published as a special issue of the Pergamon journal Vacuum, it comprises a carefully edited collection of chapters written by specialists in each of the techniques and includes coverage of the electron and ion spectroscopies, as well as the atom-imaging methods such as the atom probe field ion microscope and the scanning tunnelling microscope. Surface science is an important area of study since the outermost surface layers play a crucial role

  6. Nonenhanced MR angiography techniques

    International Nuclear Information System (INIS)

    Lanzman, R.S.; Kroepil, P.; Blondin, D.; Schmitt, P.

    2011-01-01

    Especially in regard to the potential risks for the development of nephrogenic systemic fibrosis (NSF) following the administration of Gadolinium-based contrast material, nonenhanced MR angiography (MRA) methods are becoming ever more important. Besides well-established time-of-flight (TOF) and phase-contrast (PC) MRA, alternative imaging techniques based on balanced steady-state free precession (bSSFP) and turbo-spin-echo (TSE) sequences are increasingly used in combination with or without arterial spin labeling (ASL) strategies. This article provides an overview of the principles and clinical values of different nonenhanced MRA techniques. In addition, recent nonenhanced MRA developments are presented. (orig.)

  7. Advanced Techniques in Biophysics

    CERN Document Server

    Arrondo, José Luis R

    2006-01-01

    Technical advancements are basic elements in our life. In biophysical studies, new applications and improvements in well-established techniques are being implemented every day. This book deals with advancements produced not only from a technical point of view, but also from new approaches that are being taken in the study of biophysical samples, such as nanotechniques or single-cell measurements. This book constitutes a privileged observatory for reviewing novel applications of biophysical techniques that can help the reader enter an area where the technology is progressing quickly and where a comprehensive explanation is not always to be found.

  8. Merchandising Techniques and Libraries.

    Science.gov (United States)

    Green, Sylvie A.

    1981-01-01

    Proposes that libraries employ modern booksellers' merchandising techniques to improve circulation of library materials. Using displays in various ways, the methods and reasons for weeding out books, replacing worn book jackets, and selecting new books are discussed. Suggestions for learning how to market and 11 references are provided. (RBF)

  9. Airway Clearance Techniques (ACTs)

    Medline Plus

    Full Text Available ... NACFC Carolyn and C Richard Mattingly Leadership in Mental Health Care Award Mary M. Kontos Award NACFC Reflections ... help your infant or child manage their lung health, watch parents of children with CF and a respiratory therapist talk about the different techniques they use for airway clearance. ... Instagram Email Find a Clinical Trial Help us blaze ...

  10. Techniques of Wastewater Treatment

    Indian Academy of Sciences (India)

    need and importance of wastewater treatment and conven- tional methods of treatment. Currently the need is to develop low power consuming and yet effective techniques to handle complex wastes. As a result, new and ... ered as supplying energy to make an active oxidizable species of the pollutants. Generally speaking ...

  11. New measurements techniques

    DEFF Research Database (Denmark)

    Torras Rosell, Antoni

    uncertainties to the measurement process. Optical techniques may help overcoming this problem because the sensing element is not a bulky instrument, but a beam of light that does not change the properties of sound. Optical methods are thus non-invasive and can thereby enhance the current state of the art...

  12. The attribute measurement technique

    International Nuclear Information System (INIS)

    MacArthur, Duncan W.; Langner, Diana; Smith, Morag; Thron, Jonathan; Razinkov, Sergey; Livke, Alexander

    2010-01-01

    Any verification measurement performed on potentially classified nuclear material must satisfy two seemingly contradictory constraints. First and foremost, no classified information can be released. At the same time, the monitoring party must have confidence in the veracity of the measurement. An information barrier (IB) is included in the measurement system to protect the potentially classified information while allowing sufficient information transfer to occur for the monitoring party to gain confidence that the material being measured is consistent with the host's declarations, concerning that material. The attribute measurement technique incorporates an IB and addresses both concerns by measuring several attributes of the nuclear material and displaying unclassified results through green (indicating that the material does possess the specified attribute) and red (indicating that the material does not possess the specified attribute) lights. The attribute measurement technique has been implemented in the AVNG, an attribute measuring system described in other presentations at this conference. In this presentation, we will discuss four techniques used in the AVNG: (1) the 1B, (2) the attribute measurement technique, (3) the use of open and secure modes to increase confidence in the displayed results, and (4) the joint design as a method for addressing both host and monitor needs.

  13. Classifying variability modeling techniques

    NARCIS (Netherlands)

    Sinnema, Marco; Deelstra, Sybren

    Variability modeling is important for managing variability in software product families, especially during product derivation. In the past few years, several variability modeling techniques have been developed, each using its own concepts to model the variability provided by a product family. The

  14. Techniques of Wastewater Treatment

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 5; Issue 12. Techniques of Wastewater Treatment - Future Technologies. Amol A Kulkarni Mugdha ... Chemical Engineering Division, University Department of Chemical Technology, Nathalal Parik Marg, Matunga, Mumbai 400 019, India. Chemical ...

  15. Electronic waste recycling techniques

    CERN Document Server

    Bernardes, Andréa

    2015-01-01

    This book presents an overview of the characterization of electronic waste. In addition, processing techniques for the recovery of metals, polymers and ceramics are described. This book serves as a source of information and as an educational technical reference for practicing scientists and engineers, as well as for students.

  16. 3. Techniques of Cartography

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 4; Issue 8. Mapmakers Techniques of Cartography. Harini Nagendra. Series Article Volume 4 Issue 8 August 1999 pp 8-15. Fulltext. Click here to view fulltext PDF. Permanent link: https://www.ias.ac.in/article/fulltext/reso/004/08/0008-0015 ...

  17. Airway Clearance Techniques (ACTs)

    Medline Plus

    Full Text Available ... and Their Families When There's More Than One Person With CF in the Same School Daily Life ... Awards and Grants Career Development Awards Research Awards Training Awards CF ... Clearance Airway Clearance Techniques (ACTs) There are different ways to clear your ...

  18. Simple Driving Techniques

    DEFF Research Database (Denmark)

    Rosendahl, Mads

    2002-01-01

    -like language. Our aim is to extract a simple notion of driving and show that even in this tamed form it has much of the power of more general notions of driving. Our driving technique may be used to simplify functional programs which use function composition and will often be able to remove intermediate data...

  19. 8. Algorithm Design Techniques

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 2; Issue 8. Algorithms - Algorithm Design Techniques. R K Shyamasundar. Series Article Volume 2 ... Author Affiliations. R K Shyamasundar1. Computer Science Group, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India ...

  20. Airway Clearance Techniques (ACTs)

    Medline Plus

    Full Text Available ... Team Your cystic fibrosis care team includes a group of CF health care professionals who partner with ... Awards and Grants Career Development Awards Research Awards Training Awards CF ... Clearance Airway Clearance Techniques (ACTs) There are different ways to clear your ...

  1. Safeguards techniques and equipment

    International Nuclear Information System (INIS)

    1997-01-01

    The current booklet is intended to give a full and balanced description of the techniques and equipment used for both nuclear material accountancy and containment and surveillance measures, and for the new safeguards measure of environmental sampling. As new verification measures continue to be developed, the material in the booklet will be periodically reviewed and updated versions issued. (author)

  2. Comparison with Other Techniques

    Science.gov (United States)

    Sacco, Giovanni Maria; Ferré, Sébastien; Tzitzikas, Yannis

    This chapter compares dynamic taxonomies with the other main approaches to information access and discusses analogies and differences. The approaches analyzed range from traditional retrieval paradigms, such as queries on structured data, to the most recent approaches, including the current effort on the Semantic Web: queries on structured data, and OLAP data analysis techniques;

  3. 8. Algorithm Design Techniques

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 2; Issue 8. Algorithms - Algorithm Design Techniques. R K Shyamasundar. Series Article Volume 2 Issue 8 August 1997 pp 6-17. Fulltext. Click here to view fulltext PDF. Permanent link: http://www.ias.ac.in/article/fulltext/reso/002/08/0006-0017 ...

  4. Art Appreciation and Technique.

    Science.gov (United States)

    Dean, Diane R.; Milam, Debora

    1985-01-01

    Presents examples of independent study units for gifted high school students in a resource room setting. Both art appreciation and technique are covered in activities concerned with media (basics of pencil, India ink, pastels, crayons, oil, acrylics, and watercolors), subject matter (landscapes, animals, the human figure), design and illustration…

  5. Airway Clearance Techniques (ACTs)

    Medline Plus

    Full Text Available ... Depression and CF Working With Your Care Team Fitness and Nutrition Fitness Nutrition and Your Digestive System Germs and Staying ... Techniques Autogenic Drainage Basics of Lung Care Chest Physical Therapy Coughing and Huffing High-Frequency Chest Wall ...

  6. 3. Techniques of Cartography

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 4; Issue 8. Mapmakers Techniques of Cartography. Harini Nagendra. Series Article Volume 4 Issue 8 August 1999 pp 8-15. Fulltext. Click here to view fulltext PDF. Permanent link: http://www.ias.ac.in/article/fulltext/reso/004/08/0008-0015 ...

  7. Microwave Breast Imaging Techniques

    DEFF Research Database (Denmark)

    Zhurbenko, Vitaliy; Rubæk, Tonny

    2010-01-01

    This paper outlines the applicability of microwave radiation for breast cancer detection. Microwave imaging systems are categorized based on their hardware architecture. The advantages and disadvantages of various imaging techniques are discussed. The fundamental tradeoffs are indicated between...... various requirements to be fulfilled in the design of an imaging system for breast cancer detection and some strategies to overcome these limitations....

  8. Nuclear techniques in agriculture

    International Nuclear Information System (INIS)

    Bhagwat, S.G.

    2012-01-01

    Crops provide us food grains and many other products. Demand for food and other agricultural products is increasing. There is also need for improvement of quality of the agricultural produce. There are several technologies in use for achieving the goal of increasing the quantity and quality of agricultural produce. Nuclear techniques provide us with an option which has certain advantages. The characteristics of crop plants are determined by the genetic make up of the plant. Traditionally the genetic make up was modified using conventional breeding techniques such as cross breeding to improve crops for yield, disease resistance, stress tolerance, resistance to insect pests or to improve quality. New varieties of crops are produced which replace the earlier ones and thus the demands are met. The process of development of new varieties is long and time consuming. Nuclear technique called mutation breeding provides an efficient way of breeding new varieties or improving the older ones. This technique merely enhances the process of occurrence of mutations. In nature mutations occur at a rate of approximately one in a million, while when mutations are induced using radiations such as gamma rays the efficiency of inducing mutations is enhanced. Useful mutations are selected, the mutants are evaluated and developed as a new variety. In the Nuclear Agriculture and Biotechnology Division (NA and BTD) this technique has been used to develop mutants of many crop plants. The mutants can be used to develop a variety directly or by using it in further breeding programme. Using these approaches the NA and BTD has developed 40 new varieties of crops such as groundnut, mungbean, urid, pigeon pea, mustard, soybean, sunflower, cowpea, jute. These varieties are developed in collaboration with other agricultural institutions and are popular among the farming community. The method of mutation breeding can be applied to many other crops for improvement. There is increasing interest among

  9. Managerial Techniques in Educational Administration.

    Science.gov (United States)

    Lane, John J.

    1983-01-01

    Management techniques developed during the past 20 years assume the rational bureaucratic model. School administration requires contingent techniques. Quality Circle, Theory Z, and the McKenzie 7-Framework are discussed as techniques to increase school productivity. (MD)

  10. Network acceleration techniques

    Science.gov (United States)

    Crowley, Patricia (Inventor); Awrach, James Michael (Inventor); Maccabe, Arthur Barney (Inventor)

    2012-01-01

    Splintered offloading techniques with receive batch processing are described for network acceleration. Such techniques offload specific functionality to a NIC while maintaining the bulk of the protocol processing in the host operating system ("OS"). The resulting protocol implementation allows the application to bypass the protocol processing of the received data. Such can be accomplished this by moving data from the NIC directly to the application through direct memory access ("DMA") and batch processing the receive headers in the host OS when the host OS is interrupted to perform other work. Batch processing receive headers allows the data path to be separated from the control path. Unlike operating system bypass, however, the operating system still fully manages the network resource and has relevant feedback about traffic and flows. Embodiments of the present disclosure can therefore address the challenges of networks with extreme bandwidth delay products (BWDP).

  11. Laser beam shaping techniques

    Energy Technology Data Exchange (ETDEWEB)

    DICKEY,FRED M.; WEICHMAN,LOUIS S.; SHAGAM,RICHARD N.

    2000-03-16

    Industrial, military, medical, and research and development applications of lasers frequently require a beam with a specified irradiance distribution in some plane. A common requirement is a laser profile that is uniform over some cross-section. Such applications include laser/material processing, laser material interaction studies, fiber injection systems, optical data image processing, lithography, medical applications, and military applications. Laser beam shaping techniques can be divided into three areas: apertured beams, field mappers, and multi-aperture beam integrators. An uncertainty relation exists for laser beam shaping that puts constraints on system design. In this paper the authors review the basics of laser beam shaping and present applications and limitations of various techniques.

  12. Signal integrity characterization techniques

    CERN Document Server

    Bogatin, Eric

    2009-01-01

    "Signal Integrity Characterization Techniques" addresses the gap between traditional digital and microwave curricula all while focusing on a practical and intuitive understanding of signal integrity effects within the data transmission channel. High-speed interconnects such as connectors, PCBs, cables, IC packages, and backplanes are critical elements of differential channels that must be designed using today's most powerful analysis and characterization tools.Both measurements and simulation must be done on the device under test, and both activities must yield data that correlates with each other. Most of this book focuses on real-world applications of signal integrity measurements - from backplane for design challenges to error correction techniques to jitter measurement technologies. The authors' approach wisely addresses some of these new high-speed technologies, and it also provides valuable insight into its future direction and will teach the reader valuable lessons on the industry.

  13. [Molecular techniques in mycology].

    Science.gov (United States)

    Rodríguez-Tudela, Juan Luis; Cuesta, Isabel; Gómez-López, Alicia; Alastruey-Izquierdo, Ana; Bernal-Martínez, Leticia; Cuenca-Estrella, Manuel

    2008-11-01

    An increasing number of molecular techniques for the diagnosis of fungal infections have been developed in the last few years, due to the growing prevalence of mycoses and the length of time required for diagnosis when classical microbiological methods are used. These methods are designed to resolve the following aspects of mycological diagnosis: a) Identification of fungi to species level by means of sequencing relevant taxonomic targets; b) early clinical diagnosis of invasive fungal infections; c) detection of molecular mechanisms of resistance to antifungal agents; and d) molecular typing of fungi. Currently, these methods are restricted to highly developed laboratories. However, some of these techniques will probably be available in daily clinical practice in the near future.

  14. Miniaturization Techniques for Accelerators

    Energy Technology Data Exchange (ETDEWEB)

    Spencer, James E.

    2003-05-27

    The possibility of laser driven accelerators [1] suggests the need for new structures based on micromachining and integrated circuit technology because of the comparable scales. Thus, we are exploring fully integrated structures including sources, optics (for both light and particle) and acceleration in a common format--an accelerator-on-chip (AOC). Tests suggest a number of preferred materials and techniques but no technical or fundamental roadblocks at scales of order 1 {micro}m or larger.

  15. Laparoscopic adrenalectomy: Surgical techniques

    Directory of Open Access Journals (Sweden)

    Matthew J Mellon

    2008-01-01

    Full Text Available Since its first description in 1992, laparoscopic adrenalectomy has become the gold standard for the surgical treatment of most adrenal conditions. The benefits of a minimally invasive approach to adrenal resection such as decreased hospital stay, shorter recovery time and improved patient satisfaction are widely accepted. However, as this procedure becomes more widespread, critical steps of the operation must be maintained to ensure expected outcomes and success. This article reviews the surgical techniques for the laparoscopic adrenalectomy.

  16. Electronic Packaging Techniques

    Science.gov (United States)

    1979-01-01

    A characteristic of aerospace system design is that equipment size and weight must always be kept to a minimum, even in small components such as electronic packages. The dictates of spacecraft design have spawned a number of high-density packaging techniques, among them methods of connecting circuits in printed wiring boards by processes called stitchbond welding and parallel gap welding. These processes help designers compress more components into less space; they also afford weight savings and lower production costs.

  17. Pipeline Leak Detection Techniques

    Directory of Open Access Journals (Sweden)

    Timur Chis, Ph.D., Dipl.Eng.

    2007-01-01

    Full Text Available Leak detection systems range from simple, visual line walking and checking ones to complex arrangements of hard-ware and software. No one method is universally applicable and operating requirements dictate which method is the most cost effective. The aim of the paper is to review the basic techniques of leak detection that are currently in use. The advantages and disadvantages of each method are discussed and some indications of applicability are outlined.

  18. Pipeline Leak Detection Techniques

    OpenAIRE

    Timur Chis, Ph.D., Dipl.Eng.

    2007-01-01

    Leak detection systems range from simple, visual line walking and checking ones to complex arrangements of hard-ware and software. No one method is universally applicable and operating requirements dictate which method is the most cost effective. The aim of the paper is to review the basic techniques of leak detection that are currently in use. The advantages and disadvantages of each method are discussed and some indications of applicability are outlined.

  19. Nuclear techniques in hydrology

    International Nuclear Information System (INIS)

    Bahadur, J.; Saxena, R.K.

    1974-01-01

    Several types of sealed radioactive sources, stable isotopes and water soluble radioactive tracers, used by different investigators, have been listed for studying the dynamic behaviour of water in nature. In general, all the facets of hydrological cycle, are amenable to these isotopic techniques. It is recommended that environmental isotopes data collection should be started for studying the water balance and also the interrelationships between surface and subsurface water in various rivers catchments with changing physical, geological and climatic parameters. (author)

  20. Management of science technique

    International Nuclear Information System (INIS)

    Bae, Byeong Han; Lee, Gap Du

    2002-08-01

    This book mentions change of environment and management of technique, business environment in information age technology and management such as classification of technology, innovation of technology and meaning of technology management, item innovation and technology about meaning of item development, innovation and item development, creation of item concept and item development, process of product innovation, product activity, product innovation product innovation and technology, development of product innovation, technology and marketing innovation, innovation of skill of marketing information system and globalization.

  1. Resin infiltration transfer technique

    Science.gov (United States)

    Miller, David V [Pittsburgh, PA; Baranwal, Rita [Glenshaw, PA

    2009-12-08

    A process has been developed for fabricating composite structures using either reaction forming or polymer infiltration and pyrolysis techniques to densify the composite matrix. The matrix and reinforcement materials of choice can include, but are not limited to, silicon carbide (SiC) and zirconium carbide (ZrC). The novel process can be used to fabricate complex, net-shape or near-net shape, high-quality ceramic composites with a crack-free matrix.

  2. Techniques for Wireless Applications

    KAUST Repository

    Gaaloul, Fakhreddine

    2012-05-01

    Switching techniques have been first proposed as a spacial diversity techniques. These techniques have been shown to reduce considerably the processing load while letting multi-antenna systems achieve a specific target performance. In this thesis, we take a different look at the switching schemes by implementing them for different other wireless applications. More specifically, this thesis consists of three main parts, where the first part considers a multiuser environment and an adaptive scheduling algorithm based on the switching with post-selection scheme for statistically independent but non-identically distributed channel conditions. The performance of this switched based scheduler is investigated and a multitude of performance metrics are presented. In a second part, we propose and analyze the performance of three switched-based algorithms for interference reduction in the downlink of over-loaded femtocells. For instance, performance metrics are derived in closed-form and these metrics are used to compare these three proposed schemes. Finally in a third part, a switch based opportunistic channel access scheme is proposed for a cognitive radio system and its performance is analyzed in terms of two new proposed metrics namely the average cognitive radio access and the waiting time duration.

  3. Covariance mapping techniques

    Science.gov (United States)

    Frasinski, Leszek J.

    2016-08-01

    Recent technological advances in the generation of intense femtosecond pulses have made covariance mapping an attractive analytical technique. The laser pulses available are so intense that often thousands of ionisation and Coulomb explosion events will occur within each pulse. To understand the physics of these processes the photoelectrons and photoions need to be correlated, and covariance mapping is well suited for operating at the high counting rates of these laser sources. Partial covariance is particularly useful in experiments with x-ray free electron lasers, because it is capable of suppressing pulse fluctuation effects. A variety of covariance mapping methods is described: simple, partial (single- and multi-parameter), sliced, contingent and multi-dimensional. The relationship to coincidence techniques is discussed. Covariance mapping has been used in many areas of science and technology: inner-shell excitation and Auger decay, multiphoton and multielectron ionisation, time-of-flight and angle-resolved spectrometry, infrared spectroscopy, nuclear magnetic resonance imaging, stimulated Raman scattering, directional gamma ray sensing, welding diagnostics and brain connectivity studies (connectomics). This review gives practical advice for implementing the technique and interpreting the results, including its limitations and instrumental constraints. It also summarises recent theoretical studies, highlights unsolved problems and outlines a personal view on the most promising research directions.

  4. Vibration transducer calibration techniques

    Science.gov (United States)

    Brinkley, D. J.

    1980-09-01

    Techniques for the calibration of vibration transducers used in the Aeronautical Quality Assurance Directorate of the British Ministry of Defence are presented. Following a review of the types of measurements necessary in the calibration of vibration transducers, the performance requirements of vibration transducers, which can be used to measure acceleration, velocity or vibration amplitude, are discussed, with particular attention given to the piezoelectric accelerometer. Techniques for the accurate measurement of sinusoidal vibration amplitude in reference-grade transducers are then considered, including the use of a position sensitive photocell and the use of a Michelson laser interferometer. Means of comparing the output of working-grade accelerometers with that of previously calibrated reference-grade devices are then outlined, with attention given to a method employing a capacitance bridge technique and a method to be used at temperatures between -50 and 200 C. Automatic calibration procedures developed to speed up the calibration process are outlined, and future possible extensions of system software are indicated.

  5. Computer Assisted Audit Techniques

    Directory of Open Access Journals (Sweden)

    Eugenia Iancu

    2007-01-01

    Full Text Available From the modern point of view, audit takes intoaccount especially the information systems representingmainly the examination performed by a professional asregards the manner for developing an activity by means ofcomparing it to the quality criteria specific to this activity.Having as reference point this very general definition ofauditing, it must be emphasized that the best known segmentof auditing is the financial audit that had a parallel evolutionto the accountancy one.The present day phase of developing the financial audithas as main trait the internationalization of the accountantprofessional. World wide there are multinational companiesthat offer services in the financial auditing, taxing andconsultancy domain. The auditors, natural persons and auditcompanies, take part at the works of the national andinternational authorities for setting out norms in theaccountancy and auditing domain.The computer assisted audit techniques can be classified inseveral manners according to the approaches used by theauditor. The most well-known techniques are comprised inthe following categories: testing data techniques, integratedtest, parallel simulation, revising the program logics,programs developed upon request, generalized auditsoftware, utility programs and expert systems.

  6. Empirical techniques in finance

    CERN Document Server

    Bhar, Ramaprasad

    2005-01-01

    This book offers the opportunity to study and experience advanced empi- cal techniques in finance and in general financial economics. It is not only suitable for students with an interest in the field, it is also highly rec- mended for academic researchers as well as the researchers in the industry. The book focuses on the contemporary empirical techniques used in the analysis of financial markets and how these are implemented using actual market data. With an emphasis on Implementation, this book helps foc- ing on strategies for rigorously combing finance theory and modeling technology to extend extant considerations in the literature. The main aim of this book is to equip the readers with an array of tools and techniques that will allow them to explore financial market problems with a fresh perspective. In this sense it is not another volume in eco- metrics. Of course, the traditional econometric methods are still valid and important; the contents of this book will bring in other related modeling topics tha...

  7. Enseignement technique/Technical Training

    CERN Multimedia

    1998-01-01

    Bureautique et techniques administratives/Office Automation & Administrative Techniques - Premiers pas/Getting started - E-Mail - Management Tools - WWW - Applications bureautiques/Office applications

  8. BACTERIOLOGICAL CHARACTERISTICS OF WASHED AND UNWASHED LAYING HEN’S EGGS CARACTERÍSTICAS BACTERIOLÓGICAS DE OVOS LAVADOS E NÃO LAVADOS DE GRANJAS DE PRODUÇÃO COMERCIAL

    Directory of Open Access Journals (Sweden)

    Maria Luiza Ferreira Stringhini

    2009-12-01

    Full Text Available To assess the bacteriological quality of washed and unwashed eggs obtained in four commercial poultry farms located in the metropolitan area of Goiânia, Goiás, Brazil was the purpose of this study. Farms 1 and 2 were considered as medium and small production capacity, respectively, and washed mechanically the eggs with heated water containing sanitizing while farms 3 and 4 produced unwashed eggs. The sample universe was made up of 576 eggs from farms 1 and 2, 288 obtained from the poultry facilities and other 288 collected in the classification hall, and 132 obtained in farms 3 and 4, half of this collected in the facilities and the other half in the classification hall. It was considered commercial eggs classified as large eggs from 30 to 40 weeks of age Dekalb White hens. The experimental design was a completely randomized design in a factorial arrangement 2 x 2 (washing procedure x collection place with three replicates and the experimental unit consisted of pool of six eggs. Counts of mesophilic and positive Staphylococcus coagulase and Most Probable Number (MPN of total and fecal coliforms in shells and internal content of eggs and Salmonella spp. research in eggshells were made. Results of the counts were expressed as log CFU/g and “t” Student test (5% adopted. Descriptive and frequency analysis was used to analyse MPN of total and faecal coliforms. Counts of mesophilic and positive Staphylococcus coagulase in eggshells of washed eggs in the classification hall of the farm 1 were lower (p <0.05 than that eggs collected in hen’s facilities which showed the efficiency of sanitization and good bacteriological quality of eggshell. The eggshells obtained in the classification hall of the farm 3 showed a higher (p <0.05 counts of mesophilic than in the facilities. Despite the high counting for positive Staphylococcus coagulase in the classification halls of farms 3 and 4, these values are lower than those needed to form toxin capable of

  9. Diagnostic accuracy of a standardized scheme for identification of Streptococcus uberis in quarter milk samples: A comparison between conventional bacteriological examination, modified Rambach agar medium culturing, and 16S rRNA gene sequencing.

    Science.gov (United States)

    Wald, Regina; Baumgartner, Martina; Urbantke, Verena; Stessl, Beatrix; Wittek, Thomas

    2017-02-01

    Bacteriological examination of milk samples is a prerequisite for pathogen-specific therapy and aids in limiting antimicrobial resistance. The aims of this study were to establish a standardized scheme for reliable Streptococcus uberis identification in routine diagnosis and to evaluate the accuracy of conventional tests and growing patterns of Strep. uberis on a selective medium (modified Rambach agar medium, MRAM) using 16S rRNA gene sequencing analysis as a reference method. We obtained isolates of presumptive Strep. uberis (n = 336) from quarter milk samples of dairy cows with intramammary infections and classified the isolates into 2 clusters using biochemical characterization. In cluster 1 (n = 280), cocci grew as non-hemolytic colonies, hydrolyzing esculin, carrying no Lancefield antigen (A/B/C/D/G) or Christie Atkins Munch-Petersen factor, and their growth was inhibited on an Enterococcus agar. Production of β-d-galactosidase on MRAM was shown by 257 of the cluster 1 isolates (91.79%), and 16S rRNA gene sequencing verified 271 (96.79%) of the isolates to be Strep. uberis. In 264 isolates (94.29%), MRAM agreed with the sequencing results. In cluster 2 (n = 56), isolates showed different characteristics: 37 (66.07%) were β-d-galactosidase-positive, and based on 16S sequencing results, 36 (64.29%) were identified correctly as Strep. uberis using biochemical methods. Identification success in this group differed significantly between routine diagnosis and MRAM application: MRAM agreed with sequencing results in 47 isolates (83.93%). To identify Strep. uberis and differentiate it from other lactic acid bacteria in routine diagnosis, we suggest using catalase reaction, hemolysis, esculin hydrolysis, and growth on enterococci agar. Isolates that show a typical biochemical profile can be identified satisfactorily with these tests. For Strep. uberis isolates with divergent patterns, application of MRAM as a follow-up test increased the diagnostic accuracy to 94

  10. A serological and bacteriological survey of dogs to detect Brucella infection in Lomas de Zamora, Buenos Aires province Estudio serológico y bacteriológico de brucelosis en perros de Lomas de Zamora, provincia de Buenos Aires

    Directory of Open Access Journals (Sweden)

    G. López

    2009-06-01

    Full Text Available Canine brucellosis caused by Brucella canis is a disease of the reproductive tract that may cause miscarriage in females, infection of the sexual organs in males and infertility in both sexes. The prevalence of brucellosis in dogs is unknown and little has been done to control the disease, except in certain breeds and some commercial dog kennels. In the course of a free neuter program in Lomas de Zamora, Buenos Aires province, prevalence of antibodies to Brucella sp., bacteriological isolation and clinical observations were performed. Of 224 dogs studied, 33 (14.7% were found positive for the rapid slide agglutination test (RSAT, 24 (10.7% of which were confirmed by IELISA. Of the 33 RSAT positive, 17 (51.5% blood cultures were done, and B. canis were isolated from 2 cases. Since infected dogs have been shown to remain bacteremic for prolonged periods, our results also suggest a risk of human infections in this area.La brucelosis canina causada por Brucella canis es una infección que afecta el tracto reproductivo de los perros y que puede provocar abortos en las hembras, infección de los órganos sexuales en los machos e infertilidad en ambos sexos. Se desconoce la prevalencia de esta enfermedad y las medidas de control se aplican sólo en algunos criaderos comerciales. Durante un programa de castración gratuita de perros llevado a cabo en el Partido de Lomas de Zamora (Gran Buenos Aires se realizaron estudios clínicos, serológicos y bacteriológicos en 224 animales. En la población estudiada, la prueba de microaglutinación rápida en portaobjetos (RSAT resultó positiva en suero de 33 (14,7% perros y fueron confirmadas por IELISA en 24 (10,7% de ellos. A 17 de los 33 perros positivos mediante RSAT (51,5% se les practicaron hemocultivos y en 2 casos se aisló B. canis. Debido a que los perros infectados permanecen bacteriémicos durante períodos prolongados, constituyen un riesgo para la salud pública.

  11. The efficiency of active cycle of breathing techniques regarding the improvement the quality of life in cystic fibrosis patients

    Directory of Open Access Journals (Sweden)

    Bogdan Almăjan-Guţă

    2008-12-01

    Full Text Available Background: Physiotherapy is well known as one of the most important part of CF patient’s management. The right choice ofappropriate therapy schema will improve the life’s quality of the patients. The purpose of the study was to prove the efficiencyof Active cycle of breathing techniques at children with cystic fibrosis. The study was performed between September 2006-september 2007 and the lot of study consisted of 20 children (11 girls and 9 boys with an age range between 6 and 18 years(average 14,8 years from the records of the Cystic Fibrosis National Centre Timisoara. The results showed an improvement inall measured values: general well-being, coughing, physical signs, X-ray signs and CT, bacteriological exam, nutritional status,functional respiratory tests. The statistical briefing of data shows the fact that there are significant statistical difference (p<0,05, before and after treatment in all ventilator index. The conclusion of this study was that the chosen technique (ACTBproved to be very efficient, in improving of respiratory symptoms and ventilator parameters

  12. Multimodality imaging techniques.

    Science.gov (United States)

    Martí-Bonmatí, Luis; Sopena, Ramón; Bartumeus, Paula; Sopena, Pablo

    2010-01-01

    In multimodality imaging, the need to combine morphofunctional information can be approached by either acquiring images at different times (asynchronous), and fused them through digital image manipulation techniques or simultaneously acquiring images (synchronous) and merging them automatically. The asynchronous post-processing solution presents various constraints, mainly conditioned by the different positioning of the patient in the two scans acquired at different times in separated machines. The best solution to achieve consistency in time and space is obtained by the synchronous image acquisition. There are many multimodal technologies in molecular imaging. In this review we will focus on those multimodality image techniques more commonly used in the field of diagnostic imaging (SPECT-CT, PET-CT) and new developments (as PET-MR). The technological innovations and development of new tracers and smart probes are the main key points that will condition multimodality image and diagnostic imaging professionals' future. Although SPECT-CT and PET-CT are standard in most clinical scenarios, MR imaging has some advantages, providing excellent soft-tissue contrast and multidimensional functional, structural and morphological information. The next frontier is to develop efficient detectors and electronics systems capable of detecting two modality signals at the same time. Not only PET-MR but also MR-US or optic-PET will be introduced in clinical scenarios. Even more, MR diffusion-weighted, pharmacokinetic imaging, spectroscopy or functional BOLD imaging will merge with PET tracers to further increase molecular imaging as a relevant medical discipline. Multimodality imaging techniques will play a leading role in relevant clinical applications. The development of new diagnostic imaging research areas, mainly in the field of oncology, cardiology and neuropsychiatry, will impact the way medicine is performed today. Both clinical and experimental multimodality studies, in

  13. Applied ALARA techniques

    International Nuclear Information System (INIS)

    Waggoner, L.O.

    1998-01-01

    The presentation focuses on some of the time-proven and new technologies being used to accomplish radiological work. These techniques can be applied at nuclear facilities to reduce radiation doses and protect the environment. The last reactor plants and processing facilities were shutdown and Hanford was given a new mission to put the facilities in a safe condition, decontaminate, and prepare them for decommissioning. The skills that were necessary to operate these facilities were different than the skills needed today to clean up Hanford. Workers were not familiar with many of the tools, equipment, and materials needed to accomplish:the new mission, which includes clean up of contaminated areas in and around all the facilities, recovery of reactor fuel from spent fuel pools, and the removal of millions of gallons of highly radioactive waste from 177 underground tanks. In addition, this work has to be done with a reduced number of workers and a smaller budget. At Hanford, facilities contain a myriad of radioactive isotopes that are 2048 located inside plant systems, underground tanks, and the soil. As cleanup work at Hanford began, it became obvious early that in order to get workers to apply ALARA and use hew tools and equipment to accomplish the radiological work it was necessary to plan the work in advance and get radiological control and/or ALARA committee personnel involved early in the planning process. Emphasis was placed on applying,ALARA techniques to reduce dose, limit contamination spread and minimize the amount of radioactive waste generated. Progress on the cleanup has,b6en steady and Hanford workers have learned to use different types of engineered controls and ALARA techniques to perform radiological work. The purpose of this presentation is to share the lessons learned on how Hanford is accomplishing radiological work

  14. Pileup Mitigation Techniques

    CERN Document Server

    Klein, Matthew Henry; The ATLAS collaboration

    2016-01-01

    We report on recent progress in the ATLAS experiment in developing tools to mitigate the effects of pile-up. Forward pile-up jet tagging techniques, as well as constituent-level pile-up suppression algorithms are discussed in details. The impacts of these approaches on both jet energy and angular resolution, as well as jet substructure and boosted object tagging performance are discussed. Improvements to various physics channels of interest are discussed and the potential future of such algorithms — both online and offline, and both at the current LHC and a future high-luminosity LHC and beyond — is considered in detail

  15. Techniques et technologies

    OpenAIRE

    Boscus, Alain; Comet, Georges; Fiocchi, Laurent; Fournier, Patrick; Morel, David; Phalip, Bruno; Pignot, Isabelle; Reveyron, Nicolas

    2014-01-01

    L’histoire des techniques est en train de réapparaître dans notre vision actuelle de l’histoire, non sans quelques difficultés, dues notamment au fait qu’histoire et histoire de l’art restent encore en retrait, dans l’appréhension concrète des technologies, par rapport aux arts et métiers ou à l’archéologie. Souhaitant ne céder ni à l’apparente fatalité des documentations inexplorées, ni à de trop belles évidences historiographiques, ce volume rassemble des auteurs travaillant aux limites de ...

  16. Electricity demand forecasting techniques

    International Nuclear Information System (INIS)

    Gnanalingam, K.

    1994-01-01

    Electricity demand forecasting plays an important role in power generation. The two areas of data that have to be forecasted in a power system are peak demand which determines the capacity (MW) of the plant required and annual energy demand (GWH). Methods used in electricity demand forecasting include time trend analysis and econometric methods. In forecasting, identification of manpower demand, identification of key planning factors, decision on planning horizon, differentiation between prediction and projection (i.e. development of different scenarios) and choosing from different forecasting techniques are important

  17. High voltage test techniques

    CERN Document Server

    Kind, Dieter

    2001-01-01

    The second edition of High Voltage Test Techniques has been completely revised. The present revision takes into account the latest international developments in High Voltage and Measurement technology, making it an essential reference for engineers in the testing field.High Voltage Technology belongs to the traditional area of Electrical Engineering. However, this is not to say that the area has stood still. New insulating materials, computing methods and voltage levels repeatedly pose new problems or open up methods of solution; electromagnetic compatibility (EMC) or components and systems al

  18. Imaging Techniques and Indications.

    Science.gov (United States)

    Mahoney, James M

    2017-04-01

    This article evaluates the utility of radiography, ultrasonography, and MRI in diagnosing Achilles tendon injuries. It reviews the pertinent anatomy of the Achilles and associated structures, and signs of disorder with each imaging technique. The economics of use ultrasonography and MRI are discussed. They should serve as complementary diagnostic tools, with ultrasonography the first choice because of its ease of use, ability to view dynamic function, and cost. However, clinical examination is often best for diagnosis; MRI and ultrasonography often should be considered only when the diagnosis is confounding or a patient does not respond to recommended conservative care. Copyright © 2016 Elsevier Inc. All rights reserved.

  19. Western Blot Techniques.

    Science.gov (United States)

    Kim, Brianna

    2017-01-01

    The Western blot is an important laboratory technique that allows for specific identification and characterization of proteins. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-separated proteins are electophoretically transferred to a polyvinylidene fluoride (PVDF) membrane which is then incubated with specific antibodies, then developed to show the protein of interest. Here, we describe the transfer and detection of Outer surface protein A (OspA), a protein only found on the surface of Borrelia burgdorferi, the bacteria responsible for Lyme disease.

  20. Quantitative molecular and culture analyses of bacterial elimination in oval-shaped root canals by a single-file instrumentation technique.

    Science.gov (United States)

    Alves, F R F; Rôças, I N; Almeida, B M; Neves, M A S; Zoffoli, J; Siqueira, J F

    2012-09-01

    Bacterial reduction in oval-shaped root canals by a single-instrument technique was compared ex vivo with a conventional nickel-titanium rotary technique. Data obtained from two quantification methods, quantitative real-time polymerase chain reaction (qPCR) and culture, were also compared. Oval-shaped canals of extracted teeth contaminated with Enterococcus faecalis were instrumented using either a single Reciproc instrument or the BioRaCe instrument series. Bacteriological samples were taken before (S1) and after instrumentation (S2). Bacterial quantification was performed using qPCR and culture. Intragroup analysis showed that both protocols promoted a highly significant bacterial reduction (P 0.05). As for the quantification methods, qPCR revealed significantly higher counts of E. faecalis in S1 than culture (P 0.05). The single-file technique was comparable with the conventional technique in oval-shaped canals provided the width of apical preparation, volume of irrigants and duration of irrigation are kept similar. No significant difference was observed for qPCR and culture in post-instrumentation samples, indicating that both methods can be reliably used for studies of antibacterial effectiveness. © 2012 International Endodontic Journal.

  1. Physicochemical and bacteriological characteristics of rainwater ...

    African Journals Online (AJOL)

    TSS and TDS were highest in samples collected from Asbestos rooftop, followed by Aluminum rooftop while samples from galvanized Iron had the least irrespective of the rainfall event. Rainwater samples collected at the onset of rain had higher Ca2+ concentration than those collected at the peak of rain for all roof type with ...

  2. Physicochemical and Bacteriological Assessment of Tannery ...

    African Journals Online (AJOL)

    ADOWIE PERE

    The tanning process generates wastes that are of serious environmental impact particularly in countries where environmental regulations are not sufficiently strict. The used and non-useable hides and skins along with the excess process chemicals and water constitute solid and liquid wastes in the tannery (Mingshu et al.,.

  3. original article bacteriological and physicochemical quality

    African Journals Online (AJOL)

    user

    2011-03-01

    Mar 1, 2011 ... used without treatment, it presents a sanitary risk (4). Inadequate ... water supply, and unhygienic waste management ..... home. A similar drinking water quality assessment study in Ethiopia showed that the majority of household water containers met the recommended limit of WHO and ES and classified as ...

  4. Physicochemical, bacteriological and metal analysis of some ...

    African Journals Online (AJOL)

    This study was investigates the physicochemical, microbial and metal contents of water samples obtained from three different surface waters (Ala river, Onyearugbulem stream and FUTA stream).The samples were collected in the early the early morning during the late rainy season of August 2010. The streams are all ...

  5. 42 CFR 493.911 - Bacteriology.

    Science.gov (United States)

    2010-10-01

    ... anaerobic bacteria from any source. (b) Program content and frequency of challenge. To be approved for... sensitivity or resistance to the common antimicrobial agents. (c) Evaluation of a laboratory's performance...

  6. Bacteriological Screening of Water in Mangalore, India

    OpenAIRE

    Dhanya V. C.; Anitha J.; Dhanashree B.

    2013-01-01

    Introduction: Diarrhoea caused by contaminated water is among the most prevalent waterborne diseases in the developing countries like India. In the interest of public health, water supplies should be tested regularly to confirm their freedom from contamination. Objective: The objectives of the study were to screen different water sources for bacterial contamination, to know the antibiotic susceptibility of the common bacterial isolates and typing of the bacterial isolates by ra...

  7. Bacteriological analysis of Periplaneta americana L. (Dictyoptera ...

    African Journals Online (AJOL)

    The results indicate that the amounts of bacteria were different between the districts. Moreover, the bacteria isolated from body of American cockroaches differed from those of the houseflies. These findings show that American cockroaches and houseflies may carry pathogenic bacteria in the urban areas of Tangier.

  8. bacteriological quality of water samples in

    African Journals Online (AJOL)

    met by water obtained from rainfall, streams, means of preventing pollution of the water well, boreholes or tap depending on the ... Most organized consumption of bottled and sachet water in a society depends on piped treated water to ... bottles containing 3% sodium thiosulphate solution (6). The pH and suspended solid.

  9. Physicochemical and bacteriological quality assessment of the ...

    African Journals Online (AJOL)

    ALAKEH

    measured using argentometric method (silver nitrate titration). Total nitrogen exists in three forms, namely: Nitrate-Nitrogen (. -N),. Ammonium-Nitrogen (NH4 ..... may have resulted from lead acid batteries usage, use of electronic equipment or plumbing activities. Lead is damaging to the nervous system. This equally leads ...

  10. Bacteriology of humans: an ecological perspective

    National Research Council Canada - National Science Library

    Wilson, Michael, 1947 Apr. 12

    2008-01-01

    ..., Staphylococcus aureus, etc. What is now a-changing is that interest is shifting towards the vast majority of our microbial partners that do not cause disease and, indeed, are essential to our well being. The indigenous microbiota of healthy humans is now the subject of intense scrutiny, and its enormous diversity and the crucial roles that it pl...

  11. Physicochemical and bacteriological evaluation of underground ...

    African Journals Online (AJOL)

    ... Shigella, and Proteus species, which are known to be pathogens, causing serious injuries to human health and may result to deaths in some cases, especially among children. Public health implications were advocated to reduce the increasing cases water borne diseases in the area. Keywords: contamination; pathogens ...

  12. Programa de Bacteriología

    Directory of Open Access Journals (Sweden)

    Inés del Socorro Bedoya Ortiz

    2007-01-01

    Full Text Available En este artículo se presentan los resultados de la investigación cualitativadescriptiva “Comprensión de las inteligencias develadas en el aula”. Su propósito fue comprender las inteligencias develadas en los estudiantes en el proceso enseñanza aprendizaje de la asignatura de Hematología. Se hace referencia a los contextos que convalidan la investigación, se describe paso a paso la manera cómo fue pensado y desarrollado el proyecto, señalando la población participante, las etapas del proceso, las técnicas e instrumentos de recolección de información y el tipo de investigación; se presenta el acercamiento teórico que demandó el trabajo y que se fue construyendo y reconstruyendo acorde con los hallazgos encontrados en el proceso de interacción entre estudiantes, docente e investigadora. Finalmente, se plantea una reflexión educativa a manera de recomendación, en la que se invita a los docentes a repensar, a reorientar la práctica educativa y al reto de proponer nuevos paradigmas de interpretación y de acción frente a la ciencia y a la vida misma.

  13. Bacteriological physicochemical quality of recreational water bodies ...

    African Journals Online (AJOL)

    tinsae

    Key words: Swimming pools, microbiological indicators, microbial water quality, recreational water quality. INTRODUCTION. Recreational waters ... exposure, the type of water, geographical location and local conditions. Perhaps, the ... from two natural lakes and six different outdoor swimming pools that are located at Addis ...

  14. Physicochemical and bacteriological quality assessment of the ...

    African Journals Online (AJOL)

    In order to ascertain water quality for human consumption, physical and chemical parameters, together with faecal forms of bacteria were evaluated in the drinking water resources of the Bambui community in the North West region of Cameroon. This study was necessitated by the occasional presence of suspended ...

  15. Bacteriological, physicochemical and mineral studies on Awedele ...

    African Journals Online (AJOL)

    EJIRO

    physicochemical quality of the water samples was also assessed. The mineral content of the adjacent soil samples was also determined. Antibiotic resistance among isolated Enterococcus species was highest to penicillin as all isolates demonstrated complete resistance to the antibiotics. Prevalence of gentamycin-.

  16. Bacteriological physicochemical quality of recreational water bodies ...

    African Journals Online (AJOL)

    tinsae

    Key words: Swimming pools, microbiological indicators, microbial water quality, recreational water quality. INTRODUCTION. Recreational ... quality of the selected recreational waters bodies in Addis. Ababa and Oromiya Region in ..... Le Chevallier MW, Evans TM, Seidler RJ (1981). Effect of Turbidity on. Chlorination ...

  17. Basic laboratory procedures in clinical bacteriology

    National Research Council Canada - National Science Library

    Vandepitte, J

    2003-01-01

    ... of laboratory resources Collection and transport of stool specimens Visual examination of stool specimens Enrichment and inoculation of stool specimens Media for enteric pathogens Primary isolation Prelimin...

  18. Bronchiectasis: a bacteriological profile | Bopaka | Pan African ...

    African Journals Online (AJOL)

    Pan African Medical Journal. Journal Home · ABOUT · Advanced Search · Current Issue · Archives · Journal Home > Vol 22, No 1 (2015) >. Log in or Register to get access to full text downloads. Username, Password, Remember me, or Register · Download this PDF file. The PDF file you selected should load here if your ...

  19. Bacteriological water quality changes and substratespecificity of ...

    African Journals Online (AJOL)

    Identification test revealed the presence of Pseudomonas, Proteus, Micrococcus, Escherichia, Chromobacterium, Serratia, Klebsiella, Bacillus, Staphylococcus, Lactobacillus, Pediococcus, Norcadia, Vibro, Salmonella, Aeromonas, Cytophaga, Corynebacterium, Campylobacter and unidentified gram-positive cocci. About 50 ...

  20. Isotope techniques for hydrology

    International Nuclear Information System (INIS)

    1964-01-01

    In the body of the Panel's report specific conclusions and recommendations are presented in the context of each subject. The general consensus of the Panel is as follows: by the study of this report, the 1961 Panel report, the Proceedings of the March 1963 Tokyo Symposium and other reports of research and technological advances, isotope-technique applications to hydrologic problems have provided some useful avenues for understanding the nature of the hydrologic cycle and in the solution of specific engineering problems. Some techniques are developed thoroughly enough for fairly routine application as tools for use in the solution of practical problems, but further research and development is needed on other concepts to determined whether or not they can be beneficially applied to either research or engineering problems. A concerted effort is required on the part of both hydrologists and isotope specialists working as teams to assure that proper synthesis of scientific advances in the respective fields and translation of these advances into practical technology is achieved

  1. A Technique: Exposure Therapy

    Directory of Open Access Journals (Sweden)

    Serkan AKKOYUNLU

    2013-07-01

    Full Text Available Introduction: Exposure with response prevention is an effective treatment for all anxiety disorders. According to the behavioral learning theories, fears which are conditioned via classical conditioning are reinforced by respondent conditioning. Avoidance and safety seeking behaviors prevent disconfirmation of anxious beliefs. In exposure client faces stimulates or cues that elicit fear or distress, by this avoidance is inhibited. Clients are also encouraged to resists performing safety seeking behaviors or rituals that they utilize to reduce fear or distress. Accomplishing these habituation or extinction is achieved. In addition to this clients learn that feared consequences does not realize or not harmful as they believed by experiencing. Emotional processing is believed to be the mechanism of change in exposure.Objective: The aim of this review is to provide a definition of exposure and its effectiveness briefly, and describe how to implement exposure, its steps and remarkable aspects using. Exposure therapies and treatments that involve exposure are proved to be effective in all anxiety disorders. Exposure therapy can be divided in three parts: Assessment and providing a treatment rationale, creating an exposure hierarchy and response prevention plan, implementing exposure sessions. Clients must also continue to perform exposure between sessions. Therapy transcripts are also provided to exemplify these parts. Conclusion: Exposure with response prevention is a basic and effective technique. Every cognitive behavior therapist must be able to implement this technique and be cognizant of pearls of this procedure.

  2. Single-borehole techniques

    International Nuclear Information System (INIS)

    Klotz, D.; Moser, H.; Trimborn, P.

    1978-01-01

    Proceeding on the theoretical considerations and on the experience and practice derived from laboratory and field testing, a system consisting of tracer injection units, detector units, measuring probe units and packers is presented, from which the different borehole probes required can be combined. A couple of examples of recent applications shows the position of the Single-Borehole Techniques with respect to the traditional methods used for the measurement of the ground-water flow. A confrontation of the permeabilities of different aquifers consents, both on the basis of the Single-Borehole Techniques as by pumping experiments, the determination of the reliability of the Point-Dilution-Method. The Point-Dilution-Method is giving information about the vertical and horizontal distribution of the permeabilities in an aquifer. By measuring the vertical current in two karst wells, the tributary horizons of a well have been determined, which gave valuable information for the subsequent well construction. Local leakages could be detected by measuring the vertical flow rate through observation wells arranged along a grout curtain erected on both sides of the retaining barrage of the Keban dam. (orig.) [de

  3. Techniques de hacking

    CERN Document Server

    Erickson, Jon

    2008-01-01

    Dans cet ouvrage, Jon Erickson présente les bases de la programmation en C du point de vue du hacker et dissèque plusieurs techniques de hacking, passées et actuelles, afin de comprendre comment et pourquoi elles fonctionnent. Même si vous ne savez pas programmer, ce livre vous donnera une vue complète de la programmation, de l'architecture des machines, des communications réseau et des techniques de hacking existantes. Associez ces connaissances à l'environnement Linux fourni et laissez libre cours à votre imagination. Avec ce livre vous apprendrez à : • programmer les ordinateurs en C, en assembleur et avec des scripts shell ; • inspecter les registres du processeur et la mémoire système avec un débogueur afin de comprendre précisément ce qui se passe ; Vous découvrirez comment les hackers parviennent à : • corrompre la mémoire d'un système, en utilisant les débordements de tampons et les chaînes de format, pour exécuter un code quelconque ; • surpasser les mesures de sécurit...

  4. Evolution of radiotherapeutic techniques

    International Nuclear Information System (INIS)

    Brady, L.W.; Lewis, G.C. Jr.; Antoniades, J.; Prasasvinichai, S.; Torpie, R.J.; Asbell, S.O.; Glassburn, J.R.; Schatanoff, D.; MacMurray, T.

    1974-01-01

    Adenocarcinoma is the most common kind of tumor involving the uterine corpus, occurring in a frequency of 90 percent or better and found predominantly in post-menopausal women. Because of vaginal bleeding, diagnosis is made early and control rates are good. Regional lymph node metastases are found to occur in about 20 percent of all operable patients and more frequently in those with advanced lesions near the cervix. Surgery alone fails not only because of metastases but also because of persistence in the vaginal vault and in the periurethral region. From a theoretical and practical viewpoint, preoperative irradiation is a valuable and important role in the treatment of carcinoma of the endometrium. Treatment calls for a technique that will effectively irradiate the uterine tissue, the vaginal vault, and and the immediate extrauterine tissues in which postsurgical persistence is known to appear. From the standpoint of survival, both preoperative external therapy and preoperative radium therapy are effective with equivalent survival figures. The incidence in our experience of vaginal recurrence in those patients who were irradiated preoperatively with external beam therapy techniques would indicate the need for supplemental radium within the vaginal vault

  5. Underwater cutting techniques developments

    International Nuclear Information System (INIS)

    Bach, F.-W.

    1990-01-01

    The primary circuit structures of different nuclear powerplants are constructed out of stainless steels, ferritic steels, plated ferritic steels and alloys of aluminium. According to the level of the specific radiation of these structures, it is necessary for dismantling to work with remote controlled cutting techniques. The most successful way to protect the working crew against exposure of radiation is to operate underwater in different depths. The following thermal cutting processes are more or less developed to work under water: For ferritic steels only - flame cutting; For ferritic steels, stainless steels, cladded steels and aluminium alloys - oxy-arc-cutting, arc-waterjet-cutting with a consumable electrode, arc-saw-cutting, plasma-arc-cutting and plasma-arc-saw. The flame cutting is a burning process, all the other processes are melt-cutting processes. This paper explains the different techniques, giving a short introduction of the theory, a discussion of the possibilities with the advantages and disadvantages of these processes giving a view into the further research work in this interesting field. (author)

  6. Information hiding technique

    Science.gov (United States)

    Younger, Michael; Budulas, Peter P.; Young, Stuart H.

    2002-08-01

    Spread spectrum communication techniques have been recognized as a viable method to gain an advantage in interference environments. Many military-oriented systems have been initiated, and some civil systems have been attempted. Spread spectrum allows the ability to hide the signal of interest below or in the noise floor, so as not to be detected. A spread spectrum system is one in which the transmitted signal is spread over a wide frequency band, much wider, in fact, than the minimum bandwidth required to transmit the information being sent. We at Army Research Lab (ARL) are proposing using the same technique on the Internet with port hopping. The information would be transmitted in data packets over multiple ports. The port used would vary per packet or per session basis. This port hopping gives you and the recipients the ability to take datagram's and spread them out over a multitude of ports. This will hide information among the Internet noise. This will allow trusted communications between the transmitter and receiver because of the port coding sequence. There are 64K possible ports to span datagram. Jamming of transmission would be limiting the ability of the sniffer/listener. Also, the listener will find it difficult to use a man in the middle attach, since the data will be spread over multiple ports and only the receiver and transmitter will know the specific port sequencing for the datagram.

  7. Macroscopia, histopatologia e bacteriologia de fígados de frangos (Gallus gallus condenados no abate Gross, microscopic and bacteriologic evaluations of broiler chicken livers (Gallus gallus condemned at slaughter

    Directory of Open Access Journals (Sweden)

    Aleverson da Silva Barcelos

    2006-04-01

    infecção bacteriana nos fígados condenados.Gross, microscopic, and bacteriologic evaluations of broiler chicken livers condemned at slaughter were performed. One hundred broiler livers were sampled. Ninety of them had gross lesions and were condemned by the meat inspection service. Ten of them were grossly normal. The sampling was done in two processing plants located in the State of Rio Grande do Sul, Brazil. For the gross evaluation, the following parameters were considered: shape, color, size, texture, odor, and the presence of other visible lesions. Livers with changes in color, shape, size and/or texture were 47/90; brown-pale livers with other associated macroscopic lesions were 19/90; yellow or yellowish livers with other associated gross lesions were 5/90; and green or greenish livers with other associated lesions were 19/90. The main microscopic diagnoses consisted of multifocal heterophilic colangio-hepatitis, degeneration and/or centrilobular to bridging hepatocellular necrosis, random necrotizing hepatitis and multifocal heterophylic pericolangitis. The bacteriological evaluation was oriented to finding of Escherichia coli, Salmonella spp. and Staphylococcus sp. It was carried out by direct culture of the liver samples in selective culture media. E. coli was isolated in 26/100 samples and Staphylococcus sp. in 24/100 samples. For culturing of Salmonella spp., the conventional method indicated for this bacteria was utilized. However, there was no isolation of any species of the Salmonella genus. In all the three evaluations, a high incidence of lesions suggestive of bacterial infections was observed in the condemned livers.

  8. Technique murale picturale

    Directory of Open Access Journals (Sweden)

    Claude Coupry

    2009-09-01

    Full Text Available Après des études remarquables publiées, entre autres, dans Peindre à Auxerre au Moyen Âge, IXe-XIVe siècles, l’étude technique et analytique des peintures murales n’avait pas donné lieu à de nouvelles recherches méthodologiques en dépit des travaux de terrain (Souvigny, Stavelot… où l’archéologie du bâti avait permis des rencontres entre archéologues, historiens de l’art et restaurateurs particulièrement impliqués pour la période qui nous intéresse, de la basse Antiquité au haut Moyen Âge.Ce...

  9. Diagnostic radiology - focussing technique

    International Nuclear Information System (INIS)

    Wicke, L.

    1983-01-01

    The material presented in this book has been grouped under two main headings, namely General Aspects and Technical Aspects. The first part, dealing with more general aspects, discusses the occupational background of X-ray medical technicians, places of work and equipment. This is supplemented by an outline of contrast media, exposure tables, explanation of terms for movement and positioning, and of symbols. X-ray films, and how they ought to be used and handled, are discussed and hints are given as to the preparations to be taken prior to X-ray examinations. The part on Technical Aspects deals with focussing techniques for the most current X-ray examinations, presenting drawings and symbols. This part is subdivided into various chapters in accordance with the various body regions such as skull, spine, pelvis, lower extremities, osseous thorax, upper extremities, thorax, and abdomen. (MG) [de

  10. The Sterile Insect Technique

    International Nuclear Information System (INIS)

    Kiragu, J.

    2006-01-01

    Insect pests have caused an increasing problem in agriculture and human health through crop losses and disease transmission to man and livestock. Intervention to ensure food security and human health has relied on Integrated Pest Management (IPM) strategies to keep the pests population below economic injury levels. IPM integrate a variety of methods, but there has been over-reliance on chemical control following the discovery of insecticidal properties of DDT. It is now realized that, maintaining pest populations at controlled levels is unsustainable and eradication options is now being considered. Although the Sterile Insect Technique(SIT) could be used for insect suppression, it is gaining favour in the elimination (eradication) of the target pest population through Areawide-based IPM (Author)

  11. Advanced enrichment techniques

    International Nuclear Information System (INIS)

    Johnson, A.

    1988-01-01

    BNFL is in a unique position in that it has commercial experience of diffusion enrichment, and of centrifuge enrichment through its associate company Urenco. In addition BNFL is developing laser enrichment techniques as part of a UK development programme in this area. The paper describes the development programme which led to the introduction of competitive centrifuge enrichment technology by Urenco and discusses the areas where improvements have and will continue to be made in the centrifuge process. It also describes the laser development programme currently being undertaken in the UK. The paper concludes by discussing the relative merits of the various methods of uranium enrichment, with particular reference to the enrichment market likely to obtain over the rest of the century

  12. [Therapeutic education didactic techniques].

    Science.gov (United States)

    Valverde, Maite; Vidal, Mercè; Jansa, Margarida

    2012-10-01

    This article includes an introduction to the role of Therapeutic Education for Diabetes treatment according to the recommendations of the American Diabetes Association (ADA), the Diabetes Education Study Group (DESG) of the "European Association for Study of Diabetes (EASD) and the clinical Practice Guidelines (CPG) of the Spanish Ministry of Health. We analyze theoretical models and the differences between teaching vs. learning as well as current trends (including Internet), that can facilitate meaningful learning of people with diabetes and their families and relatives. We analyze the differences, similarities, advantages and disadvantages of individual and group education. Finally, we describe different educational techniques (metaplan, case method, brainstorming, role playing, games, seminars, autobiography, forums, chats,..) applicable to individual, group or virtual education and its application depending on the learning objective.

  13. Mouldroom techniques for teletherapy

    International Nuclear Information System (INIS)

    1999-01-01

    Mould room techniques are necessary to get the best from teletherapy treatment. They are directed at: Ensuring that the part of the patient being treated remains in the same position from start to end of a fraction of radiotherapy; Ensuring that the fields, as originally imaged and planned, can be accurately reproduced for each fraction; Ensuring that, if more than one planned volume is treated, these volumes maintain a constant, reproducible relationship to each other; Deriving contours for planning teletherapy; Facilitating accuracy of setting up individual fields with respect to position on the patient and treatment unit; Fabricating and mounting blocks or shields within any field that will adequately and reproducibly shield healthy tissue or sensitive organs; Fabricating and mounting compensators and bolus material within any field that will adequately and reproducibly modify the beam as required. Immobilisation is approached initially by assessing an anatomical region, the disease and the favoured treatment machine. The versatility of each technique to accomplish immobilisation for various purposes is addressed. Immobilisation is most commonly used in the head and neck region for the treatment of cancers of the oral and nasal passages and the treatment of brain tumours. As this part of the anatomy is very flexible, the immobilisation device must not only ensure that the head is kept in a symmetrical position, but also that the degree of flexion or extension of the neck (needed for treating a pituitary as opposed to a larynx) is maintained. Sensitive structures in that region are predominantly the eyes and spinal cord but on occasions a parotid salivary gland or part of the brain may require limitation of the dose. This may be achieved by planning the teletherapy beams with shielding blocks within selected fields

  14. Mouldroom techniques for teletherapy

    International Nuclear Information System (INIS)

    2004-01-01

    Mould room techniques are necessary to get the best from teletherapy treatment. They are directed at: Ensuring that the part of the patient being treated remains in the same position from start to end of a fraction of radiotherapy. Ensuring that the fields, as originally imaged and planned, can be accurately reproduced for each fraction. Ensuring that, if more than one planned volume is treated, these volumes maintain a constant, reproducible relationship to each other. Deriving contours for planning teletherapy. Facilitating accuracy of setting up individual fields with respect to position on the patient and treatment unit. Fabricating and mounting blocks or shields within any field that will adequately and reproducibly shield healthy tissue or sensitive organs. Fabricating and mounting compensators and bolus material within any field that will adequately and reproducibly modify the beam as required. Immobilisation is approached initially by assessing an anatomical region, the disease and the favoured treatment machine. The versatility of each technique to accomplish immobilisation for various purposes is addressed. Immobilisation is most commonly used in the head and neck region for the treatment of cancers of the oral and nasal passages and the treatment of brain tumours. As this part of the anatomy is very flexible, the immobilisation device must not only ensure that the head is kept in a symmetrical position, but also that the degree of flexion or extension of the neck (needed for treating a pituitary as opposed to a larynx) is maintained. Sensitive structures in that region are predominantly the eyes and spinal cord but on occasions a parotid salivary gland or part of the brain may require limitation of the dose. This may be achieved by planning the teletherapy beams with shielding blocks within selected fields

  15. Geological data integration techniques

    International Nuclear Information System (INIS)

    1988-09-01

    The objectives of this Technical Committee are to bring together current knowledge on geological data handling and analysis technologies as developed in the mineral and petroleum industries for geological, geophysical, geochemical and remote sensing data that can be applied to uranium exploration and resource appraisal. The recommendation for work on this topic was first made at the meeting of the NEA-IAEA Joint Group of Experts on R and D in Uranium Exploration Techniques (Paris, May 1984). In their report, processing of integrated data sets was considered to be extremely important in view of the very extensive data sets built up over the recent years by large uranium reconnaissance programmes. With the development of large, multidisciplinary data sets which includes geochemical, geophysical, geological and remote sensing data, the ability of the geologist to easily interpret large volumes of information has been largely the result of developments in the field of computer science in the past decade. Advances in data management systems, image processing software, the size and speed of computer systems and significantly reduced processing costs have made large data set integration and analysis practical and affordable. The combined signatures which can be obtained from the different types of data significantly enhance the geologists ability to interpret fundamental geological properties thereby improving the chances of finding a significant ore body. This volume is the product of one of a number of activities related to uranium geology and exploration during the past few years with the intent of bringing new technologies and exploration techniques to the IAEA Member States

  16. MFIX documentation numerical technique

    Energy Technology Data Exchange (ETDEWEB)

    Syamlal, M.

    1998-01-01

    MFIX (Multiphase Flow with Interphase eXchanges) is a general-purpose hydrodynamic model for describing chemical reactions and heat transfer in dense or dilute fluid-solids flows, which typically occur in energy conversion and chemical processing reactors. The calculations give time-dependent information on pressure, temperature, composition, and velocity distributions in the reactors. The theoretical basis of the calculations is described in the MFIX Theory Guide. Installation of the code, setting up of a run, and post-processing of results are described in MFIX User`s manual. Work was started in April 1996 to increase the execution speed and accuracy of the code, which has resulted in MFIX 2.0. To improve the speed of the code the old algorithm was replaced by a more implicit algorithm. In different test cases conducted the new version runs 3 to 30 times faster than the old version. To increase the accuracy of the computations, second order accurate discretization schemes were included in MFIX 2.0. Bubbling fluidized bed simulations conducted with a second order scheme show that the predicted bubble shape is rounded, unlike the (unphysical) pointed shape predicted by the first order upwind scheme. This report describes the numerical technique used in MFIX 2.0.

  17. Specialized financing techniques

    International Nuclear Information System (INIS)

    Shepherd, J.

    1992-01-01

    Specific financing techniques applicable to wind energy projects in Canada are discussed. A limited partnership is the classic Canadian approach to tax-advantaged financing. For a typical wind project, the limited partners would get an internal rate of return of around 8% over 20 years as well as income tax deductions on Class 34 investments. This rate can be improved if the investors borrow some of the money; they get tax-free cash flow while having deductible loan interest, raising their rate of return after taxes to ca 9-10%. Special situation investors can get to take all of the Class 34 deduction right away, raising their return up to the 12% range. These investors include principal business corporations (such as utilities or oil companies), or companies who have sold their business. A second type of financing structure is related to inflation-indexed debt. The loan is structured like a mortgage, with the annual payments indexed to inflation but nevertheless low enough to provide an early positive cash flow from the project. Other possible financing structures are the immigrant investor fund and the provincial incentive corporations

  18. A hybrid straightwire technique.

    Science.gov (United States)

    Celli, Daniel; Catalfamo, Lorenza; Gasperoni, Enrico; Deli, Roberto

    2017-09-01

    An original straightwire appliance was developed in order to easily adapt force and friction to the different stages and needs of treatment. The appliance features: (1) conventional brackets with a 0.020×0.030″ slot in the anterior area, passive self-ligating brackets with a 0.022×0.030″ slot in the lateral area, and tubes with a 0.022×0.030″ slot in the molar area; (2) archwires which adapt to the different stages of treatment and increase their section and stiffness progressively, starting with 0.014″ superelastic NiTi, passing through 0.016×0.025″ and 0.019×0.025″ heat-activated NiTi and reaching 0.019×0.025″ stainless steel working archwires; (3) use of several ligation systems which progressively increase their binding (low-friction ligatures, "O"- or "8"-shape elastomeric ligatures). Clinical cases are shown to illustrate the technique. Copyright © 2017 CEO. Published by Elsevier Masson SAS. All rights reserved.

  19. Restrictive techniques: gastric banding

    Directory of Open Access Journals (Sweden)

    Katia Cristina da Cunha

    2006-03-01

    Full Text Available Surgery for the treatment of severe obesity has a definite role onthe therapeutic armamentarium all over the world. Initiated 40years ago, bariatric surgery has already a long way thanks tohundred of surgeons, who had constantly searched for the besttechnique for the adequate control of severe obesity. Among theimportant breakthroughs in obesity surgery there is theadjustable gastric band. It is a sylastic band, inflatable andadjustable, which is placed on the top of the stomach in order tocreate a 15-20 cc pouch, with an outlet of 1.3cm. The adjustablegastric band has also a subcutaneous reservoir through whichadjustments can be made, according to the patient evolution.The main feature of the adjustable gastric band is the fact thatis minimal invasive, reversible, adjustable and placedlaparoscopically. Then greatly diminishing the surgical traumato the severe obese patient. Belachew and Favretti’s techniqueof laparoscopic application of the adjustable gastric band isdescribed and the evolution of the technique during this years,as we has been practiced since 1998. The perioperative care ofthe patient is also described, as well as the follow-up and shortand long term controls.

  20. Neutron detection technique

    CERN Document Server

    Oblath, N S

    2000-01-01

    The Sudbury Neutrino Observatory (SNO) has the ability to measure the total flux of all active flavors of neutrinos using the neutral current reaction, whose signature is a neutron. By comparing the rates of the neutral current reaction to the charged current reaction, which only detects electron neutrinos, one can test the neutrino oscillation hypothesis independent of solar models. It is necessary to understand the neutron detection efficiency of the detector to make use of the neutral current reaction. This report demonstrates a coincidence technique to identify neutrons emitted from the sup 2 sup 5 sup 2 Cf neutron calibration source. The source releases on average four neutrons when a sup 2 sup 5 sup 2 Cf nucleus spontaneously fissions. Each neutron is detected as a separate event when the neutron is captured by a deuteron, releasing a gamma ray of approximately 6.25 MeV. This gamma ray is in turn detected by the photomultiplier tube (PMT) array. By investigating the time and spatial separation between n...

  1. Dating technique tested

    International Nuclear Information System (INIS)

    Anon.

    1985-01-01

    An analytical technique for dating ground water and polar ice up to a million years old has been successfully tested by scientists at Oak Ridge National Laboratory (ORNL). The system, known as a rare gas atom counter, extends the capabilities of resonance ionization mass spectrometry to include counting single atoms of krypton-81. The counter is composed of a pulsed dye laser operated in tandem with a mass spectrometer to separate the various isotopes of krypton. In a collaborative study, ORNL scientists recently used the method for the first time to count krypton-81 in a liter of ground water removed from a sandstone aquifer near Zurich. Fewer than 1000 krypton-81 atoms were isolated from the ground water samples. According to Bernard Lehman, a collaborating geochemist at the University of Bern, this first test proved that counting the small numbers of krypton-81 atoms necessary to make an estimate of the age of water could actually be done. Among the applications of this method, Lehman says, could be improved siting of locations for the disposal of radioactive wastes

  2. Dose Reduction Techniques

    CERN Document Server

    Waggoner, L O

    2000-01-01

    As radiation safety specialists, one of the things we are required to do is evaluate tools, equipment, materials and work practices and decide whether the use of these products or work practices will reduce radiation dose or risk to the environment. There is a tendency for many workers that work with radioactive material to accomplish radiological work the same way they have always done it rather than look for new technology or change their work practices. New technology is being developed all the time that can make radiological work easier and result in less radiation dose to the worker or reduce the possibility that contamination will be spread to the environment. As we discuss the various tools and techniques that reduce radiation dose, keep in mind that the radiological controls should be reasonable. We can not always get the dose to zero, so we must try to accomplish the work efficiently and cost-effectively. There are times we may have to accept there is only so much you can do. The goal is to do the sm...

  3. Qualidade bacteriológica de queijos artesanais comercializados em estradas do litoral norte do Rio Grande do Sul, Brasil Bacteriological quality of homemade cheeses commercialised in roads of the northern coast of Rio Grande do Sul, Brazil

    Directory of Open Access Journals (Sweden)

    Cristina Bergman Zaffari

    2007-06-01

    Full Text Available O consumo de queijo artesanal, vendido em estabelecimentos de beira de estrada, é comum no Estado do Rio Grande do Sul. Geralmente estes produtos não são fabricados em acordo com as boas normas de fabricação e podem constituir perigo à saúde do consumidor. Objetivou-se, com o presente trabalho, verificar a qualidade bacteriológica de queijos artesanais comercializados em estradas litorâneas por meio da contagem de coliformes e pesquisa de Listeria spp. e Brucella spp. Foram analisados 80 queijos, sendo 62 do tipo Colonial, dez do tipo Provolone, seis do tipo Ricota e dois do tipo Caccio Cavallo. No momento da coleta, 71% das amostras não estavam sob refrigeração. Todas as amostras apresentaram contagens de coliformes totais e, destas, 62 foram testadas para a presença de coliformes fecais. Um total de 84% das amostras apresentou contagens de coliformes fecais acima de 2,73- 3,7 log.UFC g- 1 (de 500 a 5000UFC mL-1, previsto como limite máximo a ser encontrado em queijos. Dos 29 estabelecimentos, 27 tinham produtos fora destes limites. Das 80 amostras, 16% continham Listeria spp., sendo 3,7% identificadas como Listeria monocytogenes. As estações do ano influenciaram no isolamento de Listeria spp., sendo a primavera considerada a estação do ano com maior número de isolados. Brucella spp. não foram isoladas nas 80 amostras de queijos analisadas. A alta freqüência de coliformes fecais e a presença de L. monocytogenes revelam que o consumo destes queijos constitui perigo de infecção à população em geral e especialmente àquelas pessoas imunocomprometidas.The consumption of homemade cheese, which is sold in little shops along the road, is very common in the state of Rio Grande do Sul, Brazil. Generally, these products are not manufactured according to the good hygiene guidelines; and may be a risk to the consumers’ health. The aim of this research was the assessment of the bacteriological quality of homemade cheese sold in

  4. Ethical Use of Gestalt Techniques.

    Science.gov (United States)

    Given, Jane A.

    The purpose of this paper is to engender a healthy respect for Gestalt theory and techniques and the use of the techniques in the client's best interest and in the interest of positive professional and self-development in the practitioner. An overview of Gestalt techniques is provided, concentrating on the two category divisions of experiments and…

  5. Dose Reduction Techniques

    International Nuclear Information System (INIS)

    WAGGONER, L.O.

    2000-01-01

    As radiation safety specialists, one of the things we are required to do is evaluate tools, equipment, materials and work practices and decide whether the use of these products or work practices will reduce radiation dose or risk to the environment. There is a tendency for many workers that work with radioactive material to accomplish radiological work the same way they have always done it rather than look for new technology or change their work practices. New technology is being developed all the time that can make radiological work easier and result in less radiation dose to the worker or reduce the possibility that contamination will be spread to the environment. As we discuss the various tools and techniques that reduce radiation dose, keep in mind that the radiological controls should be reasonable. We can not always get the dose to zero, so we must try to accomplish the work efficiently and cost-effectively. There are times we may have to accept there is only so much you can do. The goal is to do the smart things that protect the worker but do not hinder him while the task is being accomplished. In addition, we should not demand that large amounts of money be spent for equipment that has marginal value in order to save a few millirem. We have broken the handout into sections that should simplify the presentation. Time, distance, shielding, and source reduction are methods used to reduce dose and are covered in Part I on work execution. We then look at operational considerations, radiological design parameters, and discuss the characteristics of personnel who deal with ALARA. This handout should give you an overview of what it takes to have an effective dose reduction program

  6. Advanced qualification techniques

    International Nuclear Information System (INIS)

    Winokur, P.S.; Shaneyfelt, M.R.; Meisenheimer, T.L.; Fleetwood, D.M.

    1993-01-01

    This paper demonstrates use of the Qualified Manufacturers List (QML) methodology to qualify commercial and military microelectronics for use in space applications. QML ''builds in'' the hardness of product through statistical process control (SPC) of technology parameters relevant to the radiation response, test structure to integrated circuit (IC) correlations, and techniques for extrapolating laboratory test results to low-dose-rate space scenarios. Each of these elements is demonstrated and shown to be a cost-effective alternative to expensive end-of-line IC testing. Several examples of test structured-IC correlations are provided and recent work on complications arising from transistor scaling and geometry is discussed. The use of a 10-keV x-ray wafer-level test system to support SPC and establish ''process capability'' is illustrated and a comparison of 10-keV x-ray and Co 60 gamma irradiations is provided for a wide range of CMOS technologies. The x-ray tester is shown to be cost-effective and its use in lot acceptance/qualification is recommended. Finally, a comparison is provided between MIL-STD-883D, Test Method 1019.4, which governs the testing of packaged semiconductor microcircuits in the DoD, and ESA/SSC Basic Specification No. 22900, Europe's Total Dose Steady-State Irradiation Test Method. Test Method 1019.4 focuses on conservative estimates of MOS hardness for space and tactical applications, while Basic Specification 22900 focuses on improved simulation of low-dose-rate space environments

  7. Dose Reduction Techniques

    Energy Technology Data Exchange (ETDEWEB)

    WAGGONER, L.O.

    2000-05-16

    As radiation safety specialists, one of the things we are required to do is evaluate tools, equipment, materials and work practices and decide whether the use of these products or work practices will reduce radiation dose or risk to the environment. There is a tendency for many workers that work with radioactive material to accomplish radiological work the same way they have always done it rather than look for new technology or change their work practices. New technology is being developed all the time that can make radiological work easier and result in less radiation dose to the worker or reduce the possibility that contamination will be spread to the environment. As we discuss the various tools and techniques that reduce radiation dose, keep in mind that the radiological controls should be reasonable. We can not always get the dose to zero, so we must try to accomplish the work efficiently and cost-effectively. There are times we may have to accept there is only so much you can do. The goal is to do the smart things that protect the worker but do not hinder him while the task is being accomplished. In addition, we should not demand that large amounts of money be spent for equipment that has marginal value in order to save a few millirem. We have broken the handout into sections that should simplify the presentation. Time, distance, shielding, and source reduction are methods used to reduce dose and are covered in Part I on work execution. We then look at operational considerations, radiological design parameters, and discuss the characteristics of personnel who deal with ALARA. This handout should give you an overview of what it takes to have an effective dose reduction program.

  8. Advanced qualification techniques

    Energy Technology Data Exchange (ETDEWEB)

    Winokur, P.S; Shaneyfelt, M.R.; Meisenheimer, T.L.; Fleetwood, D.M.

    1993-12-01

    This paper demonstrates use of the Qualified Manufacturers List (QML) methodology to qualify commercial and military microelectronics for use in space applications. QML ``builds in`` the hardness of product through statistical process control (SPC) of technology parameters relevant to the radiation response, test structure to integrated circuit (IC) correlations, and techniques for extrapolating laboratory test results to low-dose-rate space scenarios. Each of these elements is demonstrated and shown to be a cost-effective alternative to expensive end-of-line IC testing. Several examples of test structured-IC correlations are provided and recent work on complications arising from transistor scaling and geometry is discussed. The use of a 10-keV x-ray wafer-level test system to support SPC and establish ``process capability`` is illustrated and a comparison of 10-keV x-ray and Co{sup 60} gamma irradiations is provided for a wide range of CMOS technologies. The x-ray tester is shown to be cost-effective and its use in lot acceptance/qualification is recommended. Finally, a comparison is provided between MIL-STD-883D, Test Method 1019.4, which governs the testing of packaged semiconductor microcircuits in the DoD, and ESA/SSC Basic Specification No. 22900, Europe`s Total Dose Steady-State Irradiation Test Method. Test Method 1019.4 focuses on conservative estimates of MOS hardness for space and tactical applications, while Basic Specification 22900 focuses on improved simulation of low-dose-rate space environments.

  9. Ultra high speed image processing techniques. [electronic packaging techniques

    Science.gov (United States)

    Anthony, T.; Hoeschele, D. F.; Connery, R.; Ehland, J.; Billings, J.

    1981-01-01

    Packaging techniques for ultra high speed image processing were developed. These techniques involve the development of a signal feedthrough technique through LSI/VLSI sapphire substrates. This allows the stacking of LSI/VLSI circuit substrates in a 3 dimensional package with greatly reduced length of interconnecting lines between the LSI/VLSI circuits. The reduced parasitic capacitances results in higher LSI/VLSI computational speeds at significantly reduced power consumption levels.

  10. Handbook of Forecasting Techniques. Part I. List of 73 Techniques

    Science.gov (United States)

    1977-08-01

    differently by most practitioners. Techniques such ab meditation, certain games, psychedelic drugs , biofeedback, yoga, etc., often help in producing...m ~TIP JA\\’AB’fY 2E I lllltlt l! I i List of 73 Forecasting Techniques Cost- Benefit Analysis ,Statistical Models (Bayesian) Marginal Analysis...Steiner 1969, p. 412 FORECASTING EVALUATION FORM #1 What is it? Name: BENEFIT -COST ANALYSIS Definition/description: A technique to measure the coats of an

  11. Theorists and Techniques: Connecting Education Theories to Lamaze Teaching Techniques.

    Science.gov (United States)

    Podgurski, Mary Jo

    2016-01-01

    Should childbirth educators connect education theory to technique? Is there more to learning about theorists than memorizing facts for an assessment? Are childbirth educators uniquely poised to glean wisdom from theorists and enhance their classes with interactive techniques inspiring participant knowledge and empowerment? Yes, yes, and yes. This article will explore how an awareness of education theory can enhance retention of material through interactive learning techniques. Lamaze International childbirth classes already prepare participants for the childbearing year by using positive group dynamics; theory will empower childbirth educators to address education through well-studied avenues. Childbirth educators can provide evidence-based learning techniques in their classes and create true behavioral change.

  12. Ultrasonic techniques for fluids characterization

    CERN Document Server

    Povey, Malcolm J W

    1997-01-01

    This book is a comprehensive and practical guide to the use of ultrasonic techniques for the characterization of fluids. Focusing on ultrasonic velocimetry, the author covers the basic topics and techniques necessaryfor successful ultrasound measurements on emulsions, dispersions, multiphase media, and viscoelastic/viscoplastic materials. Advanced techniques such as scattering, particle sizing, and automation are also presented. As a handbook for industrial and scientific use, Ultrasonic Techniques for Fluids Characterization is an indispensable guide to chemists and chemical engineers using ultrasound for research or process monitoring in the chemical, food processing, pharmaceutical, cosmetic, biotechnology,and fuels industries. Key Features * Appeals to anyone using ultrasound to study fluids * Provides the first detailed description of the ultrasound profiling technique for dispersions * Describes new techniques for measuring phase transitions and nucleation, such as water/ice and oil/fat * Presents the l...

  13. Luminescence techniques: Instrumentation and methods

    DEFF Research Database (Denmark)

    Bøtter-Jensen, L.

    1997-01-01

    This paper describes techniques, instruments and methods used in luminescence dating and environmental dosimetry in many laboratories around the world. These techniques are based on two phenomena - thermally stimulated luminescence and optically stimulated luminescence. The most commonly used...... luminescence stimulation and detection techniques are reviewed and information is given on recent developments in instrument design and on the stale of the art in luminescence measurements and analysis. (C) 1998 Elsevier Science Ltd. All rights reserved....

  14. Informationization nuclear apparatus communication technique

    International Nuclear Information System (INIS)

    Yu Tiqi; Fang Zongliang; Wen Qilin

    2006-01-01

    The paper explains the request of communication ability in nuclear technique application area. Based on the actuality of nuclear apparatus communication ability, and mainly combining with the development of communication technique, the authors analyzes the application trend of communication technique applying in nuclear apparatus, for the apparatus and system needing communication ability, they need selecting suitable communication means to make them accomplish the task immediately and effectively. (authors)

  15. Radar interferometry persistent scatterer technique

    CERN Document Server

    Kampes, Bert M

    2014-01-01

    This volume is devoted to the Persistent Scatterer Technique, the latest development in radar interferometric data processing. It is the only book on Permanent Scatterer (PS) technique of radar interferometry, and it details a newly developed stochastic model and estimator algorithm to cope with possible problems for the application of the PS technique. The STUN (spatio-temporal unwrapping network) algorithm, developed to cope with these issues in a robust way, is presented and applied to two test sites.

  16. Optical pulses, lasers, measuring techniques

    CERN Document Server

    Früngel, Frank B A

    1965-01-01

    High Speed Pulse Technology: Volume II: Optical Pulses - Lasers - Measuring Techniques focuses on the theoretical and engineering problems that result from the capacitor discharge technique.This book is organized into three main topics: light flash production from a capacitive energy storage; signal transmission and ranging systems by capacitor discharges and lasers; and impulse measuring technique. This text specifically discusses the air spark under atmospheric conditions, industrial equipment for laser flashing, and claims for light transmitting system. The application of light impulse sign

  17. Some Diagnosis and Therapy Techniques in Rainbow Trout (Oncorhynchus Mykiss Furunculosis, in Intensive System Bred

    Directory of Open Access Journals (Sweden)

    Octavian Negrea

    2010-10-01

    Full Text Available Clinical and paraclinical investigations (morpho-pathologic, bacterioscopic, bacteriologic and sensitiveness test toantibodies done on 20 fish (rainbow trout, dead or in agony state, taken from a furunculosis pesthole in Salmonidaeand proceeded from an intensive exploitation unit of trout, in Cluj county, put in evidence the following aspects :Toclinical exam, in breeding basins, it appears individuals (5 % which have presented locomotor troubles, listless anduntidy swimming in water surface, lateral swimming anorexia, and on tegument it is observed diffuse hemorrhagiczones, lost of fish scale and necrosis of fins. It appears also secondary infections with fungi of Saprolegnia genusunder the form of a white- dirty, downy film. From the 20 trut corpses were isolated 2 bacterial strains, from anteriorkidney and from raclage in fin basis, with congestive lesions. Bacteria isolated are developing only after anincubation at 20 – 250 C and does not grow up to 370 C, and to microscopic exam directly from sauce, the mobility isabsent Classic biochemical tests put in evidence the following positive biochemic properties: cataloze, oxidaze,indolal and the presence of hemolytic activity. Biochemical properties testing on API20E gallery also puts inevidence properties as : positive gelatinousis and sucraze fermentation. Based on bacterial strains developmentisolated only to 20 - 250C and the above mentioned biochemic properties, respectively the absence of pigment,bacterial strains isolated are appointed in Aeromonas genus, Aeromonas salmonicida species, achromogenessubspecies. By sensitiveness test in different medicine substances, using antibiogram technique, bacterial strainsisolated from pesthole are sensitive in a decreasing order to: nalidixic acid, oxitetracycline, florfenicol andeurofloxacin and resistant to ampicyline, amoxiclav and colistin.

  18. Comparison Of Clustering Techniques And A Univariate Technique ...

    African Journals Online (AJOL)

    In further analysis of clustering technique and with the application of either complete linkage or further neighbour linkage methods, there was a positive perfect correlation on the memberships of the groups. Therefore, any of these two techniques could be used to group locations. The only difference is that clustering ...

  19. Developing Fighting Technique Through Visualization

    Directory of Open Access Journals (Sweden)

    Tim Lajcik

    2012-07-01

    Full Text Available Visualization is a training technique that involves creating a detailed mental “movie” of successful performance. This article describes a type of visualization called “mental rehearsal” and explains how it can be used to reinforce the neuromuscular pattern of proper fighting technique. Drawing on his experience as a professional fighter and college coach, his studies in sport psychology as a college student, and his exposure to mental training techniques at the U.S. Olympic Training Center, the author reveals how to use mental imagery to facilitate the mastery of martial art technique.    

  20. GPU Pro advanced rendering techniques

    CERN Document Server

    Engel, Wolfgang

    2010-01-01

    This book covers essential tools and techniques for programming the graphics processing unit. Brought to you by Wolfgang Engel and the same team of editors who made the ShaderX series a success, this volume covers advanced rendering techniques, engine design, GPGPU techniques, related mathematical techniques, and game postmortems. A special emphasis is placed on handheld programming to account for the increased importance of graphics on mobile devices, especially the iPhone and iPod touch.Example programs and source code can be downloaded from the book's CRC Press web page. 

  1. Techniques to Bring Up Mucus

    Science.gov (United States)

    ... Sexual Activity Less Strenuous Positions for Sexual Intercourse Techniques to Bring Up Mucus COPD: Exercises Giving Up Smoking Managing Your Environment Nutrition Patients & Visitors Giving For ...

  2. Algorithms Design Techniques and Analysis

    CERN Document Server

    Alsuwaiyel, M H

    1999-01-01

    Problem solving is an essential part of every scientific discipline. It has two components: (1) problem identification and formulation, and (2) solution of the formulated problem. One can solve a problem on its own using ad hoc techniques or follow those techniques that have produced efficient solutions to similar problems. This requires the understanding of various algorithm design techniques, how and when to use them to formulate solutions and the context appropriate for each of them. This book advocates the study of algorithm design techniques by presenting most of the useful algorithm desi

  3. Parachute technique for partial penectomy

    Directory of Open Access Journals (Sweden)

    Fernando Korkes

    2010-04-01

    Full Text Available PURPOSE: Penile carcinoma is a rare but mutilating malignancy. In this context, partial penectomy is the most commonly applied approach for best oncological results. We herein propose a simple modification of the classic technique of partial penectomy, for better cosmetic and functional results. TECHNIQUE: If partial penectomy is indicated, the present technique can bring additional benefits. Different from classical technique, the urethra is spatulated only ventrally. An inverted "V" skin flap with 0.5 cm of extension is sectioned ventrally. The suture is performed with vicryl 4-0 in a "parachute" fashion, beginning from the ventral portion of the urethra and the "V" flap, followed by the "V" flap angles and than by the dorsal portion of the penis. After completion of the suture, a Foley catheter and light dressing are placed for 24 hours. CONCLUSIONS: Several complex reconstructive techniques have been previously proposed, but normally require specific surgical abilities, adequate patient selection and staged procedures. We believe that these reconstructive techniques are very useful in some specific subsets of patients. However, the technique herein proposed is a simple alternative that can be applied to all men after a partial penectomy, and takes the same amount of time as that in the classic technique. In conclusion, the "parachute" technique for penile reconstruction after partial amputation not only improves the appearance of the penis, but also maintains an adequate function.

  4. A disposition of interpolation techniques

    NARCIS (Netherlands)

    Knotters, M.; Heuvelink, G.B.M.

    2010-01-01

    A large collection of interpolation techniques is available for application in environmental research. To help environmental scientists in choosing an appropriate technique a disposition is made, based on 1) applicability in space, time and space-time, 2) quantification of accuracy of interpolated

  5. GENERALIZED PROCESSOR SHARING (GPS) TECHNIQUES

    African Journals Online (AJOL)

    Olumide

    popular technique, Generalized Processor Sharing (GPS), provided an effective and efficient utilization of the available resources at the face of stringent and varied QoS requirements. This paper, therefore, presents the comparison of two GPS techniques –. PGPS and CDGPS – based on performance with limited resources ...

  6. Software Testing Techniques and Strategies

    OpenAIRE

    Isha,; Sunita Sangwan

    2014-01-01

    Software testing provides a means to reduce errors, cut maintenance and overall software costs. Numerous software development and testing methodologies, tools, and techniques have emerged over the last few decades promising to enhance software quality. This paper describes Software testing, need for software testing, Software testing goals and principles. Further it describe about different Software testing techniques and different software testing strategies.

  7. Magnetic characterization techniques for nanomaterials

    CERN Document Server

    2017-01-01

    Sixth volume of a 40 volume series on nanoscience and nanotechnology, edited by the renowned scientist Challa S.S.R. Kumar. This handbook gives a comprehensive overview about Magnetic Characterization Techniques for Nanomaterials. Modern applications and state-of-the-art techniques are covered and make this volume an essential reading for research scientists in academia and industry.

  8. Diagram Techniques in Group Theory

    Science.gov (United States)

    Stedman, Geoffrey E.

    2009-09-01

    Preface; 1. Elementary examples; 2. Angular momentum coupling diagram techniques; 3. Extension to compact simple phase groups; 4. Symmetric and unitary groups; 5. Lie groups and Lie algebras; 6. Polarisation dependence of multiphoton processes; 7. Quantum field theoretic diagram techniques for atomic systems; 8. Applications; Appendix; References; Indexes.

  9. Software Agent Techniques in Design

    DEFF Research Database (Denmark)

    Hartvig, Susanne C

    1998-01-01

    This paper briefly presents studies of software agent techniques and outline aspects of these which can be applied in design agents in integrated civil engineering design environments.......This paper briefly presents studies of software agent techniques and outline aspects of these which can be applied in design agents in integrated civil engineering design environments....

  10. TECHNIQUES FOR TEACHING CONSERVATION EDUCATION.

    Science.gov (United States)

    BROWN, ROBERT E.; MOUSER, G.W.

    CONSERVATION PRINCIPLES, FIELD METHODS AND TECHNIQUES, AND SPECIFIC FIELD LEARNING ACTIVITIES ARE INCLUDED IN THIS REFERENCE VOLUME FOR TEACHERS. CONSERVATION PRINCIPLES INCLUDE STATEMENTS PERTAINING TO (1) SOIL, (2) WATER, (3) FOREST, AND (4) WILDLIFE. FIELD METHODS AND TECHNIQUES INCLUDE (1) PREPARING FOR A FIELD TRIP, (2) GETTING STUDENT…

  11. Microirradiation techniques in radiobiological research

    Indian Academy of Sciences (India)

    The aim of this work is to review the uses of laser microirradiation and ion microbeam techniques within the scope of radiobiological research. Laser microirradiation techniques can be used for many different purposes. In a specific condition, through the use of pulsed lasers, cell lysis can be produced for subsequent ...

  12. Alimentary tract bacteria isolated and identified with API-20E and molecular cloning techniques from Australian tropical fruit flies, Bactrocera cacuminata and B. tryoni.

    Science.gov (United States)

    Thaochan, N; Drew, R A I; Hughes, J M; Vijaysegaran, S; Chinajariyawong, A

    2010-01-01

    Bacteria were isolated from the crop and midgut of field collected Bactrocera cacuminata (Hering) and Bactrocera tryoni (Froggatt) (Diptera: Tephritidae). Two methods were used, firstly isolation onto two types of bacteriological culture media (PYEA and TSA) and identification using the API-20E diagnostic kit, and secondly, analysis of samples using the 16S rRNA gene molecular diagnostic method. Using the API-20E method, 10 genera and 17 species of bacteria in the family Enterobacteriaceae were identified from cultures growing on the nutrient agar. The dominant species in both the crop and midgut were Citrobacter freundii, Enterobacter cloacae and Klebsiella oxytoca. Providencia rettgeri, Klebsiella pneumoniae ssp ozaenae and Serratia marcescens were isolated from B. tryoni only. Using the molecular cloning technique that is based on 16S rRNA gene sequences, five bacteria classes were dignosed — Alpha-, Beta-, Gamma- and Delta- Proteobacteria and Firmicutes — including five families, Leuconostocaceae, Enterococcaceae, Acetobacteriaceae, Comamonadaceae and Enterobacteriaceae. The bacteria affiliated with Firmicutes were found mainly in the crop while the Gammaproteobacteria, especially the family Enterobacteriaceae, was dominant in the midgut. This paper presents results from the first known application of molecular cloning techniques to study bacteria within tephritid species and the first record of Firmicutes bacteria in these flies.

  13. Strongly correlated systems experimental techniques

    CERN Document Server

    Mancini, Ferdinando

    2015-01-01

    The continuous evolution and development of experimental techniques is at the basis of any fundamental achievement in modern physics. Strongly correlated systems (SCS), more than any other, need to be investigated through the greatest variety of experimental techniques in order to unveil and crosscheck the numerous and puzzling anomalous behaviors characterizing them. The study of SCS fostered the improvement of many old experimental techniques, but also the advent of many new ones just invented in order to analyze the complex behaviors of these systems. Many novel materials, with functional properties emerging from macroscopic quantum behaviors at the frontier of modern research in physics, chemistry and materials science, belong to this class of systems. The volume presents a representative collection of the modern experimental techniques specifically tailored for the analysis of strongly correlated systems. Any technique is presented in great detail by its own inventor or by one of the world-wide recognize...

  14. Theorists and Techniques: Connecting Education Theories to Lamaze Teaching Techniques

    Science.gov (United States)

    Podgurski, Mary Jo

    2016-01-01

    ABSTRACT Should childbirth educators connect education theory to technique? Is there more to learning about theorists than memorizing facts for an assessment? Are childbirth educators uniquely poised to glean wisdom from theorists and enhance their classes with interactive techniques inspiring participant knowledge and empowerment? Yes, yes, and yes. This article will explore how an awareness of education theory can enhance retention of material through interactive learning techniques. Lamaze International childbirth classes already prepare participants for the childbearing year by using positive group dynamics; theory will empower childbirth educators to address education through well-studied avenues. Childbirth educators can provide evidence-based learning techniques in their classes and create true behavioral change. PMID:26848246

  15. The Prevalence of Antibiotic Resistant Diarrhogenic Bacterial ...

    African Journals Online (AJOL)

    BACKGROUND: This study assessed the bacteriological qualities of surface waters in Afikpo, between April and September 2016. METHODS: Surface water samples were collected from three streams for bacteriological analysis. Bacteria species were isolated using standard microbiological and biochemical techniques.

  16. Food Physics and Radiation Techniques

    International Nuclear Information System (INIS)

    Szabo, A. S.

    1999-01-01

    In the lecture information is given about food physics, which is a rather new, interdisciplinary field of science, connecting food science and applied physics. The topics of radioactivity of foodstuffs and radiation techniques in the food industry are important parts of food physics. Detailed information will be given about the main fields (e.g. radio stimulation, food preservation) of radiation techniques in the agro-food sector. Finally some special questions of radioactive contamination of foodstuffs in Hungary and applicability of radioanalytical techniques (e.g. INAA) for food investigation will be analyzed and discussed

  17. Nuclear techniques in analytical chemistry

    CERN Document Server

    Moses, Alfred J; Gordon, L

    1964-01-01

    Nuclear Techniques in Analytical Chemistry discusses highly sensitive nuclear techniques that determine the micro- and macro-amounts or trace elements of materials. With the increasingly frequent demand for the chemical determination of trace amounts of elements in materials, the analytical chemist had to search for more sensitive methods of analysis. This book accustoms analytical chemists with nuclear techniques that possess the desired sensitivity and applicability at trace levels. The topics covered include safe handling of radioactivity; measurement of natural radioactivity; and neutron a

  18. A simplified indirect bonding technique

    Directory of Open Access Journals (Sweden)

    Radha Katiyar

    2014-01-01

    Full Text Available With the advent of lingual orthodontics, indirect bonding technique has become an integral part of practice. It involves placement of brackets initially on the models and then their transfer to teeth with the help of transfer trays. Problems encountered with current indirect bonding techniques used are (1 the possibility of adhesive flash remaining around the base of the brackets which requires removal (2 longer time required for the adhesive to gain enough bond strength for secure tray removal. The new simplified indirect bonding technique presented here overcomes both these problems.

  19. Scalable Techniques for Formal Verification

    CERN Document Server

    Ray, Sandip

    2010-01-01

    This book presents state-of-the-art approaches to formal verification techniques to seamlessly integrate different formal verification methods within a single logical foundation. It should benefit researchers and practitioners looking to get a broad overview of the spectrum of formal verification techniques, as well as approaches to combining such techniques within a single framework. Coverage includes a range of case studies showing how such combination is fruitful in developing a scalable verification methodology for industrial designs. This book outlines both theoretical and practical issue

  20. Statistical Techniques for Project Control

    CERN Document Server

    Badiru, Adedeji B

    2012-01-01

    A project can be simple or complex. In each case, proven project management processes must be followed. In all cases of project management implementation, control must be exercised in order to assure that project objectives are achieved. Statistical Techniques for Project Control seamlessly integrates qualitative and quantitative tools and techniques for project control. It fills the void that exists in the application of statistical techniques to project control. The book begins by defining the fundamentals of project management then explores how to temper quantitative analysis with qualitati

  1. Food physics and radiation techniques

    International Nuclear Information System (INIS)

    Szabo, A. S.

    1999-01-01

    In the lecture information is given about food physics, which is a rather new, interdisciplinary field of science, connecting food science and applied physics. The topics of radioactivity of foodstuffs and radiation techniques in the food industry are important parts of food physics detailed information will be given about the main fields (e.g. radio stimulation, food preservation) of radiation techniques in the agro-food sector. Finally some special questions of radioactive contamination of foodstuffs in hungary and applicability of radioanalytical techniques (e.g. Inaa) for food investigation will be analyzed and discussed

  2. PIGE technique implementation at ININ

    Energy Technology Data Exchange (ETDEWEB)

    Policroniades, R., E-mail: rafael.policroniades@inin.gob.mx; Martínez-Quiroz, E.; Méndez-Garrido, B.; Murillo, G.; Moreno, E.; Villaseñor, P. [Departamento de Aceleradores, Instituto Nacional de Investigaciones Nucleares (ININ), Carr. México-Toluca S/N, La Marquesa, Ocoyoacac, México CP 52750 (Mexico)

    2015-07-23

    In this work, we present a general overview about the implementation at ININ of a Particle Induced Gamma Emission (PIGE) analysis technique, based on the bombardment of samples by protons and deuterons at different energies within our tandem accelerator facility. As it is well known, this technique is based on the detection of γ-rays emitted by nuclei in a target following a charged particle irradiation. The main feature of this technique, apart from being non-destructive and low time consuming, is that it allows a multi-elemental analysis of a sample, permitting an isotopic identification of many nuclides. Advances and some preliminary results are presented.

  3. PIGE technique implementation at ININ

    International Nuclear Information System (INIS)

    Policroniades, R.; Martínez-Quiroz, E.; Méndez-Garrido, B.; Murillo, G.; Moreno, E.; Villaseñor, P.

    2015-01-01

    In this work, we present a general overview about the implementation at ININ of a Particle Induced Gamma Emission (PIGE) analysis technique, based on the bombardment of samples by protons and deuterons at different energies within our tandem accelerator facility. As it is well known, this technique is based on the detection of γ-rays emitted by nuclei in a target following a charged particle irradiation. The main feature of this technique, apart from being non-destructive and low time consuming, is that it allows a multi-elemental analysis of a sample, permitting an isotopic identification of many nuclides. Advances and some preliminary results are presented

  4. Artificial intelligence techniques in Prolog

    CERN Document Server

    Shoham, Yoav

    1993-01-01

    Artificial Intelligence Techniques in Prolog introduces the reader to the use of well-established algorithmic techniques in the field of artificial intelligence (AI), with Prolog as the implementation language. The techniques considered cover general areas such as search, rule-based systems, and truth maintenance, as well as constraint satisfaction and uncertainty management. Specific application domains such as temporal reasoning, machine learning, and natural language are also discussed.Comprised of 10 chapters, this book begins with an overview of Prolog, paying particular attention to Prol

  5. [Impressions techniques--Part 2].

    Science.gov (United States)

    Levartovsky, S; Masri, M; Alter, E; Pilo, R

    2012-10-01

    A dental impression is a positive replica of the teeth, the surrounding gingiva and the border between them; the purpose of which is to create an accurate master model. Two major techniques for impressions exist today: The conventional and the digital impressions. The current article describes both techniques. In the conventional impressions, it is important to choose a proper tray, stock or custom, and to mix the material properly. The commonly used impression techniques for making a conventional impression are described with a review on the effect of the technique on its accuracy. The effect of the wash bulk on the accuracy of the stone dies and/or the restoration is discussed, as well. The digital impressions with their advantages and disadvantages are described in comparison to the conventional impressions. Although, digital impressions eliminate some of the negative characteristics of conventional impressions, proper soft-tissue management and isolation of tooth preparation margins is still mandatory.

  6. Computer systems performance measurement techniques.

    Science.gov (United States)

    1971-06-01

    Computer system performance measurement techniques, tools, and approaches are presented as a foundation for future recommendations regarding the instrumentation of the ARTS ATC data processing subsystem for purposes of measurement and evaluation.

  7. Research Development Techniques and Initiatives.

    Science.gov (United States)

    Miller, Barry

    1986-01-01

    The growth and development of research activities at a state psychiatric research and training institute while under the new management of a local medical school is described, and specific techniques and initiatives to enhance research are outlined. (MSE)

  8. Experimental techniques and measurement accuracies

    International Nuclear Information System (INIS)

    Bennett, E.F.; Yule, T.J.; DiIorio, G.; Nakamura, T.; Maekawa, H.

    1985-02-01

    A brief description of the experimental tools available for fusion neutronics experiments is given. Attention is paid to error estimates mainly for the measurement of tritium breeding ratio in simulated blankets using various techniques

  9. Radiotracer techniques in hydrological studies

    International Nuclear Information System (INIS)

    Oladipo, M.O.A.; Funtua, I.I.

    2000-07-01

    The use of radioactive tracers particularly short-lived radioisotopes frequently offers advantages over conventional methods of analyses. Applications of nuclear techniques in the field of hydrology constitute important and sometimes unique tools for obtaining critical information needed for water resources management. Essentially, radiotracer techniques offer a safe, cost effective and powerful tool in the assessment, management and protection of water resources. The Centre for Energy Research and Training, Ahmadu Bello University, Zaria of late has been offering consultancy services to some industries in the area of radiotracer technique. The first nuclear reactor in Nigeria, the MNSR, is expected to be commissioned in the Centre very soon. Many short-lived radioisotopes such as Cu-64, Ga-72, Br-82, Hg-197 etc which are very important in hydrological studies can be produced by the MNSR facility. This article reports on the basic principles of the technique and its roles in hydrology

  10. Modern techniques of surface science

    CERN Document Server

    Woodruff, D Phil

    2016-01-01

    This fully revised, updated and reorganised third edition provides a thorough introduction to the characterisation techniques used in surface science and nanoscience today. Each chapter brings together and compares the different techniques used to address a particular research question, including how to determine the surface composition, surface structure, surface electronic structure, surface microstructure at different length scales (down to sub-molecular), and the molecular character of adsorbates and their adsorption or reaction properties. Readers will easily understand the relative strengths and limitations of the techniques available to them and, ultimately, will be able to select the most suitable techniques for their own particular research purposes. This is an essential resource for researchers and practitioners performing materials analysis, and for senior undergraduate students looking to gain a clear understanding of the underlying principles and applications of the different characterisation tec...

  11. Experimental technique of neutron reflection

    International Nuclear Information System (INIS)

    Chen Bo; Huang Chaoqiang; Li Xinxi

    2006-12-01

    It is presented that the classifications, structures and components of neutron reflectometer (NR), as well s functions and parameters of each components, detailed characters of NR facility 'PRN-2M'. Based on the practical experiments, the basic experimental techniques, the measurement and the related experimental settings are described, including the choice of experimental conditions, adjustments of polarized neutron beam line, basic experimental technique and approach of measurement. The above can be an instruction for NR experiments and a reference for NR construction. (authors)

  12. Uranium exploration techniques in Bolivia

    International Nuclear Information System (INIS)

    Virreira, V.

    1981-01-01

    The exploration techniques used by the Bolivian Nuclear Energy Commission/Comision Boliviana de Energia Nuclear (COBOEN) in certain areas of Bolivia that are considered promising from the standpoint of uranium deposits are presented in summary form. The methods and results obtained are described, including the techniques used by the Italian company AGIP-URANIUM during four years of exploration under contract with COBOEN. Statistical data are also given explaining the present level of uranium exploration in Bolivia. (author)

  13. Survey of semantic modeling techniques

    Energy Technology Data Exchange (ETDEWEB)

    Smith, C.L.

    1975-07-01

    The analysis of the semantics of programing languages was attempted with numerous modeling techniques. By providing a brief survey of these techniques together with an analysis of their applicability for answering semantic issues, this report attempts to illuminate the state-of-the-art in this area. The intent is to be illustrative rather than thorough in the coverage of semantic models. A bibliography is included for the reader who is interested in pursuing this area of research in more detail.

  14. PERSONALISED DENTURES WITH BRANCHING TECHNIQUE

    OpenAIRE

    Puneet; Priyanka

    2012-01-01

    ABSTRACT: While the basic process of making dentures has chang ed little over the past several decades, new materials and techniques can help labor atories and clinicians provide functional, esthetic restorations that offer exceptional value t o patients. Unlike the conventional “linear” methods which foll ow specific steps in a cookbook fashion, the Branching Technique is a dynamic conce pt which can be adjusted to meet the clinical needs of each patient. Thi...

  15. Infrared Devices And Techniques (Revision)

    OpenAIRE

    Rogalski A.; Chrzanowski K.

    2014-01-01

    The main objective of this paper is to produce an applications-oriented review covering infrared techniques and devices. At the beginning infrared systems fundamentals are presented with emphasis on thermal emission, scene radiation and contrast, cooling techniques, and optics. Special attention is focused on night vision and thermal imaging concepts. Next section concentrates shortly on selected infrared systems and is arranged in order to increase complexity; from image intensifier systems,...

  16. Highly reliable TOFD UT Technique

    International Nuclear Information System (INIS)

    Acharya, G.D.; Trivedi, S.A.R.; Pai, K.B.

    2003-01-01

    The high performance of the time of flight diffraction technique (TOFD) with regard to the detection capabilities of weld defects such as crack, slag, lack of fusion has led to a rapidly increasing acceptance of the technique as a pre?service inspection tool. Since the early 1990s TOFD has been applied to several projects, where it replaced the commonly used radiographic testing. The use of TOM lead to major time savings during new build and replacement projects. At the same time the TOFD technique was used as base line inspection, which enables monitoring in the future for critical welds, but also provides documented evidence for life?time. The TOFD technique as the ability to detect and simultaneously size flows of nearly any orientation within the weld and heat affected zone. TOM is recognized as a reliable, proven technique for detection and sizing of defects and proven to be a time saver, resulting in shorter shutdown periods and construction project times. Thus even in cases where inspection price of TOFD per welds is higher, in the end it will result in significantly lower overall costs and improve quality. This paper deals with reliability, economy, acceptance criteria and field experience. It also covers comparative study between radiography technique Vs. TOFD. (Author)

  17. Teaching Speaking Through Debate Technique

    Directory of Open Access Journals (Sweden)

    . Suranto

    2016-07-01

    Full Text Available Abstract : Teaching Speaking Through Debate Technique. Speaking is one of the basic competence from the other fourth basic competence (listening, speaking, reading and writing. Speaking ability should be mastered by every students, in order to achieve that competence students should be given the right technique to study sepaking. The successfull of the students speaking can be seen from their ability to express idea, thought and feeling through speaking. The objective of this Action Research is to improve students’s oral communication skill through the debate technique. This study was conducted at MA Ma’arif Nu 5 Sekampung Lampung Timur from March to April 2014. The research data were taken from students in the eleventh class, with 28 students and analyzed qualitatively and quantitatively. The research findings indicate that there are improvements in students’ english speaking skill through the debate technique. By analyzing data qualitatively and quantitatively from the end of the first cycle to the second cycle and it was found that the students’ English speaking skill increased 20,9% over the standard that has been determined by the researcher that is 65%. The researcher concludes that the students’ english speaking skill can be improve through the debate technique in learning process.   Key words : action research, debate technique, english speaking skill

  18. Microscopy techniques in flavivirus research.

    Science.gov (United States)

    Chong, Mun Keat; Chua, Anthony Jin Shun; Tan, Terence Tze Tong; Tan, Suat Hoon; Ng, Mah Lee

    2014-04-01

    The Flavivirus genus is composed of many medically important viruses that cause high morbidity and mortality, which include Dengue and West Nile viruses. Various molecular and biochemical techniques have been developed in the endeavour to study flaviviruses. However, microscopy techniques still have irreplaceable roles in the identification of novel virus pathogens and characterization of morphological changes in virus-infected cells. Fluorescence microscopy contributes greatly in understanding the fundamental viral protein localizations and virus-host protein interactions during infection. Electron microscopy remains the gold standard for visualizing ultra-structural features of virus particles and infected cells. New imaging techniques and combinatory applications are continuously being developed to push the limit of resolution and extract more quantitative data. Currently, correlative live cell imaging and high resolution three-dimensional imaging have already been achieved through the tandem use of optical and electron microscopy in analyzing biological specimens. Microscopy techniques are also used to measure protein binding affinities and determine the mobility pattern of proteins in cells. This chapter will consolidate on the applications of various well-established microscopy techniques in flavivirus research, and discuss how recently developed microscopy techniques can potentially help advance our understanding in these membrane viruses. Copyright © 2013 Elsevier Ltd. All rights reserved.

  19. Review of advanced imaging techniques

    Directory of Open Access Journals (Sweden)

    Yu Chen

    2012-01-01

    Full Text Available Pathology informatics encompasses digital imaging and related applications. Several specialized microscopy techniques have emerged which permit the acquisition of digital images ("optical biopsies" at high resolution. Coupled with fiber-optic and micro-optic components, some of these imaging techniques (e.g., optical coherence tomography are now integrated with a wide range of imaging devices such as endoscopes, laparoscopes, catheters, and needles that enable imaging inside the body. These advanced imaging modalities have exciting diagnostic potential and introduce new opportunities in pathology. Therefore, it is important that pathology informaticists understand these advanced imaging techniques and the impact they have on pathology. This paper reviews several recently developed microscopic techniques, including diffraction-limited methods (e.g., confocal microscopy, 2-photon microscopy, 4Pi microscopy, and spatially modulated illumination microscopy and subdiffraction techniques (e.g., photoactivated localization microscopy, stochastic optical reconstruction microscopy, and stimulated emission depletion microscopy. This article serves as a primer for pathology informaticists, highlighting the fundamentals and applications of advanced optical imaging techniques.

  20. Techniques for thyroid imaging; Les techniques d`imagerie thyroidienne

    Energy Technology Data Exchange (ETDEWEB)

    Hermans, J.

    1995-12-31

    Advances in imaging techniques has improved our understanding of diseases. The different imaging techniques for visualizing the thyroid parenchyma, including cyto-puncture, sonography, CT-scan, scintigraphy, magnetic resonance imaging, have provided various types of information. Do these techniques really provide the clinician with the answers to his questions. The information provided by the different imaging techniques is presented together with the insufficiencies of each method. Faced with the rising cost of health services, we developed analysis instruments which should help the clinician in a more rational use of diagnostic examinations. The question which most often arises is that of an isolated nodule within a multi-nodular goiter : is it malignant or benign. the analysis of the available techniques shows that cost-effective strategy uses conventional Tc99m or I123 scintigraphy and thallium 201 scintigraphy. With this strategy, the risk of missing a thyroid cancer is approximately 1.75%. With cyto-puncture, this risk is multiplied by a factor of 2.5 reaching 4.5%. (Author). 31 refs., 7 tabs.