Sample records for bacteries etude experimentale

  1. Hydration of swelling clay and bacteria interaction. An experimental in situ reaction study; Hydratation des argiles gonflantes et influence des bacteries. Etude experimentale de reaction in situ

    Energy Technology Data Exchange (ETDEWEB)

    Berger, J


    observed between Na- and Ca-smectite. The hydration mechanisms of compacted smectite occurring within a confined volume system are presented in a schematic model involving different scales, ranging from the Angstrom-scale of lattice layers to the bulk clay structure. Whereas the nature of interlayer cation affects hydration on all scales, the ionic strength of the infiltrating solution primarily affects the bulk texture. The impact of selected smectites (SWy-2, MX80 and nontronite) on the growth of S. putrefaciens was studied using agitated batch solution experiments combined with viable cell counts. The prolonged survival of bacteria in smectite suspensions compared to growth in culture medium is attributed to i) a continuous and sustainable supply of cationic nutrients and Corg, ii) the buffering capacity of the smectite clay and iii) the large clay surface areas, which accumulate nutrients, serve as attachment sites and sorb toxic waste products. The rate of bacterially induced smectite alteration/dissolution in batch solutions, as monitored by ICPOES and microscopy (confocal, ESEM and TEM coupled to EDX), shows depletion of the main cations in the nontronite extracted solution. This is attributed to the initial consumption and/or binding of cations by S. putrefaciens, which is probably related to the production of chelating agents. The constantly depleted Ca is most likely stored in the abundant EPS (exo-polymeric substance). Enhanced cation release in the case of nontronite in long-term experiments was especially evident for Fe and Al that corresponds to more than 10% partial dissolution. In contrast, the Fe-poor, Na-smectite was not seen to be affected by bacterial activity in this way and the increased release of Al by acid leaching corresponds to only 1.4% partial smectite dissolution. The presence of S. putrefaciens induced abundant textural changes as observed by microscopic investigations (confocal microscopy, ESEM), associated with the formation of smectite

  2. Theoretical and Experimental Methods in Hypersonic Flows (Les Methodes Theoriques et Experimentales pour l’Etude Des Ecoulements Hypersoniques) (United States)


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  3. Etude theorique et experimentale des evaporateurs de dioxyde de carbone operant dans des conditions de givrage (United States)

    Bendaoud, Adlane Larbi

    Les evaporateurs de refrigeration sont surtout du type tube a ailettes, appeles serpentins, et fonctionnent dans l'une des conditions suivantes: seche, humide ou avec formation de givre. Il a ete demontre que la formation du givre sur la paroi exterieure de l'echangeur engendre une surconsommation energetique a cause des operations de degivrage puisque 15 a 20% seulement de la chaleur produite sert au degivrage tandis que le reste est dissipee dans l'environnement [1]. Avec l'avenement des nouveaux refrigerants, moins nocifs envers l'environnement, l'industrie du froid se trouve penalisee du fait que peu ou pas de composantes mecaniques (compresseur, pompe, echangeur...etc.) adaptees sont disponibles [3]. Il s'agit pour la communaute des frigoristes de combler ce retard technologique en redeveloppant ces composantes mecaniques afin qu'elles soient adaptees aux nouveaux refrigerants. Dans cette optique, et afin de mieux comprendre le comportement thermique des evaporateurs au CO2 fonctionnant dans des conditions seches, qu'un groupe de chercheurs du CanmetENERGIE avaient lance, en 2000, un programme de R & D. Dans le cadre de programme un outil de simulation des evaporateurs au CO2 a ete developpe et un banc d'essai contenant une boucle secondaire de refrigeration utilisant le CO2 comme refrigerant a ete construit. Comme continuite de ce travail de recherche, en 2006 ce meme groupe de recherche a lance un nouveau projet qui consiste a faire une etude theorique et experimentale des evaporateurs au CO2 operants dans des conditions de givrage. Et, c'est exactement dans le cadre de ce projet que se positionne ce travail de these. Ce travail de recherche a ete entrepris pour mieux comprendre le comportement thermique et hydrodynamique des serpentins fonctionnant dans des conditions de givrage, l'effet des circuits de refrigerant ainsi que celui des parametres geometriques et d'operation. Pour cela, un travail theorique supporte par une etude experimentale a ete effectue

  4. Experimental study of the hydrodynamic instabilities occurring in boiling-water reactors; Etude experimentale des instabilites hydrodynamiques survenant dans les reacteurs nucleaires a ebullition

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    Fabreca, S. [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires


    The subjects is an experimental out-of pile loop study of the hydrodynamic oscillations occurring in boiling-water reactors. The study was carried out at atmospheric pressure and at pressure of about 8 atmospheres, in channels heated electrically by a constant and uniform specified current. In the test at 8 atmospheres the channel was a round tube of approximately 6 mm interior diameter. At 1 atmosphere a ring-section channel was used, 10 * 20 mm in diameter, with an inner heating tube and an outer tube of pyrex. It was possible to operate with natural convection and also with forced convection with test-channel by-pass. The study consists of 3 parts: 1. Preliminary determination of the laws governing pressure-drop during boiling. 2. Determination of the fronts at which oscillation appears, within a wide range of the parameters involved. 3. A descriptive study of the oscillations and measurement of the periods. The report gives the oscillation fronts with natural and forced convection for various values of the singular pressure drop at the channel inlet and for various riser lengths. The results are presented in non-dimensional form, which is available, in first approximation, for all geometric scales and for all fluids. Besides the following points were observed: - the wall (nature and thickness) can be an important factor ; - oscillation can occur in a horizontal channel. (author) [French] II a ete effectue une etude experimentale, en boucle hors-pile, des oscillations hydrodynamiques survenant dans les reacteurs a ebullition. L'etude a ete effectuee a la pression atmospherique et a une pression voisine de 8 atmospheres dans des canaux chauffes electriquement a puissance imposee constante et uniforme. Dans les essais a 8 atmospheres le canal etait un tube circulaire de diametre interieur 6 mm environ. A 1 atmosphere le canal etait de section annulaire 10 * 20 mm avec un tube interieur chauffant et un tube exterieur en pyrex. Le fonctionnement etait possible

  5. Experimental study of contamination by inhalation of radioactive iodine aerosols. Biological balance; Etude experimentale de la contamination par inhalation d'aerosols d'iode radioactif bilan biologique

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    Marble, G. [Commissariat a l' Energie Atomique, 92 - Fontenay-aux-Roses (France). Centre d' Etudes Nucleaires


    Several articles have been published concerning research into contamination produced by inhalation of radioactive iodine aerosols in monkeys. Results dealing with the biological balance of this contamination are presented and discussed in this report. (author) [French] L'etude experimentale de la contamination par inhalation d'aerosols d'iode radioactif effectuee chez le singe a fait l'objet de plusieurs publications. Les resultats concernant le bilan biologique de cette contamination sont presentes et discutes dans ce rapport. (auteur)

  6. Experimental study of heat transfer and pressures drops for cans with spiral herring-bone fins; Etude experimentale du transfert de chaleur et des pertes de charges des gaines a ailettes helicoidales en chevron

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    Pelce, J.; Francois, S.; Houseaux, O.; Pierre, B. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    Cans fitted with herring-bone fins are used for cooling uranium in certain nuclear reactor. By herring-bone is meant a staggered arrangement of the fins which have a plane of symmetry parallel to the general direction of liquid flow. The main geometrical parameter are then: the number of fins, the number of herring-bones, the angle of inclination of the fins with respect to the can axis, the dimensions of the fins, the can diameter and the channel diameter. The research is essentially experimental. The test are of three types: full size tests, in conditions approaching those in the reactor (constant flux, CO{sub 2} under pressure); full size tests but with a constant wall temperature, much easier to set up, and intended to distinguish rapidly between the merits of the various types of can; large-scale tests with air at atmospheric pressure for studying the phenomena in more detail. For each can tried out there is a corresponding pressure drop coefficient, a mean thermal exchange coefficient Mo-bar and a minimum exchange coefficient Mo{sub min} and Mo-bar are related by the expression Mo{sub min} = Mo-bar * f{sub c} * f, where f{sub c} and f are respectively circumferential and longitudinal singularity factor determined from a statistical study of all the temperatures measured for each can. The results are presented in about thirty tables and figures the most noteworthy results being summarized in the conclusion. (authors) [French] Les gaines a ailettes en chevron sont utilisees pour le refroidissement de l'uranium dans certains reacteurs nucleaires. Par chevron, on entend une disposition alternee des ailettes ayant un plan de symetrie parallele a la direction generale de l'ecoulement fluide. Les principaux parametres geometriques sont alors: le nombre des ailettes, le nombre de chevrons, l'angle d'inclinaison des ailettes par rapport a l'axe de la gaine, les dimensions des ailettes, le diametre de la gaine et le diametre du canal. L'etude

  7. Etude experimentale des parametres biologiques de callosobruchus ...

    African Journals Online (AJOL)

    Describtion of thoses factor levels was : 32°C and 60 % R.H. ; 27 °C and 70 % R.H. ; 23°C and 80 % R.H. ; pods and seeds of cowpea and of Cassia occidentalis (Linn.) and finally northern and southern strains of the insect storage pest. Various biological parameters were : intrinsic rate of increase (rm), finite rate of increase ...

  8. Theoretical and experimental study of heat transfers and pressure drops along surfaces fitted with herring-bone fins: correlation between geometric and aero thermal parameters; Etudes theorique et experimentale du transfert de chaleur et des pertes de charge de surfaces munies d'ailettes disposees en chevron - correlation entre parametres geometriques et aerothermiques

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    Pelce, J.; Malherbe, J.; Pierre, B. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    Principal results are given of experimental research which has been carried out on the flow of a fluid along a surface fitted with herringbone fins. Aero-thermal tests have been effected on a large number of these surfaces whose geometrical parameters have been made to vary systematically. In particular, work on a large scale model has made it possible to analyse the mechanisms of heat transfer and of pressure drops. On this basis a theoretical study has led to the establishment of a correlation between the geometric configuration and the aero-thermal performances of these surfaces. Experimental results are in good agreement with the theoretical relationships. An expression has thus been derived applicable to this type of herring-boned surface in a wide zone. (authors) [French] L'ecoulement d'un fluide au voisinage d'une surface munie d'ailettes disposees en chevron a fait l'objet de recherches experimentales dont on a rappele les principaux resultats. Des essais aerothermiques ont ete effectues sur un grand nombre de ces surfaces dont a fait varier les parametres geometriques de facon systematique. En particulier, des etudes sur une maquette a grande echelle ont permis d'analyser les mecanismes de transfert de chaleur et de perte de charge. Sur ces bases, une etude theorique a conduit a des correlations entre la geometrie et les performances aerothermiques de ces surfaces. Les resultats experimentaux sont en bon accord avec les relations theoriques. On possede ainsi une formulation pour ce type de surface ailettee valable dans un domaine etendu. (auteurs)

  9. Contribution to the experimental survey of the nuclear isomerism. Application of the deferred coincidences method to research and to the survey of metastable states of short period; Contribution a l'etude experimentale de l'isomerie nucleaire. Application de la methode des coincidences differees a la recherche et a l'etude d'etats metastables de periode courte

    Energy Technology Data Exchange (ETDEWEB)

    Ballini, R. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    transitions qu'ils subissent, lorsqu'elles donnent naissance a des especes nucleaires mieux connues: parmi ces transitions figurent les transitions isomeriques. Le but de ce travail est l'etude des transitions isomeriques a partir d'etats metastables de periode courte, comprise dans le domaine de la microseconde a quelques millisecondes. La methode des coincidences differees a ete mise au point et appliquee dans ce but en utilisant les avantages des selecteurs a plusieurs canaux, sous deux aspects principaux ou l'appareil a plusieurs canaux etait soit un selecteur de temps, soit un selecteur d'amplitudes. Cette methode a servi a etudier le fonctionnement de compteurs Geiger-Muller et a mesurer avec precision la periode de {sup 181}Ta{sup *} dans des conditions experimentales variees. La valeur adoptee est 17,2 {+-} 0,2 {mu}s. Ce travail a aussi trouve une application pratique immediate a la mise en evidence de quantites tres faibles de hafnium dans le zirconium, dont il constitue une impurete tenace et difficile a analyser par les moyens ordinaires. (M.B.)

  10. Radio-vulcanization of natural rubber in the latex phase. Study of an experimental 1 tonne per hour production; Radio-vulcanisation du caoutchouc naturel en phase latex. Etude d'une production experimentale de 1 tonne par heure

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    Leveque, P.; Puig, J.R.; Roudeix, H. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    After briefly reviewing the main research carried out on the radio-vulcanization of latex and elastomers, a description is given of 4 types of cell which have been used successively with a view to industrial irradiation. They have made it possible to acquire the information necessary for resolving the main problem arising during irradiation - the formation of coagulum. The first two cell are designed for irradiation by a horizontal beam ('Dynamitron'), the two others use a vertical beam ('Circe'). The study of the properties of the rubber obtained shows it to compare favorably with 'Revultex'. In the appendix are given some characteristics of natural latex and information about its processing. (authors) [French] Apres un rappel des principales etudes sur la radio-vulcanisation du latex et des elastomeres, on decrit les quatre types de cellules successivement essayes en vue de l'irradiation industrielle. Ils ont permis d'acquerir les informations necessaires a la resolution du probleme principal pose par l'irradiation, la formation de coagulum au cours de celle,-ci. Les deux premiers sont concus pour l'irradiation par un faisceau horizontal ('Dynamitron'), les derniers par un faisceau vertical ('Circe'). L'etude des proprietes du caoutchouc obtenu montre qu'il se compare favorablement au 'Revultex'. Un apercu est donne en annexe des caracteristiques du latex naturel et de sa mise en oeuvre. (auteurs)

  11. Experimental study of an ion cyclotron instability in a magnetic well confined plasma; Etude experimentale d'une instabilite cyclotronique ionique dans un plasma confine dans un puits magnetique

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    Brossier, P. [Commissariat a l' Energie Atomique, Fontenay-Aux-Roses (France). Centre d' Etudes Nucleaires


    This report is a contribution to the study of microinstabilities in macroscopically stable plasmas, in the low-{beta} limit. Ion cyclotron instabilities, with k{sub ||} = 0, have been numerically studied in detail; the computation of the density thresholds and growth rates of the different harmonics showed the relative role played by the following energy sources: density gradient, perpendicular distribution function and cold plasma component. This theoretical model has been compared with the results of a detailed study (density thresholds, wave structure, frequency spectrum, wavelengths, growth rate, amplitude of the electric field) of the instability observed in the DECA II device. This comparison gave a good agreement which shows the destabilising role played by the cold plasma component on a hot plasma with a loss cone distribution function. (author) [French] Ce rapport est une contribution a l'etude des microinstabilites dans les plasmas macroscopiquement stables, dans la limite des {beta} << 1. Les instabilites cyclotroniques ioniques, a k{sub ||} = 0, ont ete etudiees numeriquement en detail; le calcul des seuils de densite et taux de croissance des differents harmoniques a permis de preciser l'importance relative des facteurs destabilisants suivants: gradient de densite, fonction de distribution perpendiculaire, presence de plasma froid. Ce modele theorique a ete confronte avec les resultats d'une etude detaillee (seuils de densite, structure de l'onde, spectres de frequence, longueurs d'onde, taux de croissance, amplitude du champ electrique) de l'instabilite observee dans l'experience DECA II, Cette confrontation aboutit a un accord satisfaisant qui montre le role destabilisant joue par le plasma froid sur un plasma chaud presentant une distribution de cone de pertes. (auteur)

  12. Contribution to the theoretical and experimental study of the electron-volt effect in N-P junctions; Contribution a l'etude theorique et experimentale de l'effet electronvoltaique dans les jonctions N-P

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    Nguyen Van, Dong [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    , taking into account other phenomena which occur at low temperature. In the fourth part, results concerning the healing of the defects and protection measures against too energetic {beta} rays are described. A healing difference was observed between the lifetime and the conductivity. Finally, interesting results were obtained from experiments using a cadmium sulphide film to transform the {beta} particles of {sup 90}Sr-{sup 90}Y into photons. (author) [French] Le but propose dons le cadre de ce travail est d'etudier le comportement d'une jonction semi-conductrice sous l'action des rayons {beta}. L'etude a porte d'une part sur la conversion directe de l'energie rayonnee par une source radioactive en energie electrique utilisable a l'aide des jonctions N-P, et d'autre part sur la cinetique des defauts produits dans les cristaux de semi-conducteur par les rayons {beta} de grande energie. Dans la premiere partie du travail, on a essaye de completer les theories anterieures de l'effet electron-voltaique dans les jonctions en faisant une analyse mathematique de l'effet. Ceci a permis d'atteindre une equation unique dans laquelle figurent les parametres electriques et geometriques du semi-conducteur, de la jonction et les proprietes du rayonnement incident. D'autre part, on a etudie d'une maniere plus approfondie le courant de diffusion des porteurs de charge crees par le bombardement en tenant compte de tous les facteurs intervenant dans l'expression de l'efficacite de collection de charge d'une jonction. Dans la deuxieme partie, ou des experiences d'irradiation des jonctions N-P ont ete effectuees avec une source de {sup 90}Sr- {sup 90}Y, on a signale l'interet particulier d'une jonction d'arseniure de gallium susceptible de travailler a des temperatures relativement elevees (voisines de 100 deg. C). La troisieme partie presente l'etude des defauts crees dans un cristal de

  13. Theoretical and experimental study of ions focusing systems in order to improve the ions beam brightness by suppressing aberration causes; Etude theorique et experimentale de la focalisation des ions afin d'ameliorer la brillance du faisceau ionique par la suppression des causes d'aberration

    Energy Technology Data Exchange (ETDEWEB)

    Faure, J


    It is shown that a beam brightness can be improved by an order of magnitude when the sources of aberrations are suppressed in the anode region source, as well as in the extraction region and in the electrostatic focusing system. A calculation was first set up to determine a simple focusing system. The aberration ratio due to this focusing system is smaller than 10 to 15 per cent. The experimental study was developed by using an ion source and its extraction system capable of producing an aberration free beam at an energy of 20 keV and an accelerating unit up to 190 keV that confirms that the qualities of a 35 mA beam produced by the said ion source are not spoiled when the beam goes through the focusing and accelerating system that was designed to be aberration free. (author) [French] Dans ce travail, on s'attache a demontrer que la brillance d'un faisceau peut etre amelioree d'un ordre de grandeur lorsque l'on supprime les causes d'aberration aussi bien a la sortie de la source, dans la zone d'extraction, que dans le systeme de focalisation electrostatique. Un calcul est, tout d'abord, mis au point pour determiner un systeme de focalisation simple. Cette focalisation n'entraine pas un taux d'aberrations superieur a 10 ou 15 pour cent. Puis l'etude experimentale conduit: a) a la realisation d'une source et de son systeme d'extraction fournissant un faisceau sans aberration a une energie de 20 keV. b) et a l'elaboration d'un petit accelerateur a 190 keV qui verifie que les proprietes d'un faisceau de 35 mA issu de la source ne sont pas affectees par la traversee des systemes focalisateur et accelerateur lorsque ceux-ci n'apportent pas d'aberration. (auteur)

  14. Etude Experimentale du Photo-Injecteur de Fermilab

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    Carneiro, Jean-Paul [Orsay


    TESLA (TeV Superconducting Linear Accelerator) is an international collaboration which is studying the feasibility of an $e^+e^-$ collider of energy 0.8 TeV in the center of mass. One of the first goals of this collaboration was to construct a prototype linear accelerator at the DESY Laboratory in Hamburg, the TESLA Test Facility (TTF), in order to establish the technical basis for the collider. Two injectors were developed for TTF: a thermionic injector (developed by LAL-Orsay, IPN-Orsay, and CEA-Saclay) and a photo-injector (developed by Fermilab). The thermionic injector was used from February 1997 to October 1998, and then it was replaced by the photo-injector, which was first operated in December 1998. Another photo-injector, identical to the one delivered to TTF, was installed at Fermilab in the $A{\\emptyset}$ Building. The first beam from the latter was produced on 3 March 1999. The photo-injector consists of an RF gun, followed by a superconducting cavity. The RF gun is a 1.625-cell copper cavity with a resonant frequency of 1.3 GHz. The gun contains a cesium telluride ($C_{s_2}$Te) photo-cathode, which is illuminated by UV pulses from a Nd:YLF laser. The system can produce trains of 800 bunches of photo-electrons of charge 8 nC per bunch with spacing between bunches of 1$\\mu$s and 10 Hz repetition rate. Upon emerging from the RF gun, the beam energy is 4 to 5 MeV; the beam is then rapidly accelerated by the superconducting cavity to an energy of 17 to 20 MeV. Finally, a magnetic chicane, consisting of 4 dipoles, produces longitudinal compression of the electron bunches. This thesis describes the installation of the photo-injector at Fermilab and presents the experimentally-measured characteristics of the injector. The principal measurements were quantum eciency, dark current, transverse emittance, and bunch length. The conclusion from these studies is that the quality of the photo-injector beam fullls the design goals. The photo-injector at Fermilab is presently available for user experiments, including the production of at beams and plasma wake eld acceleration.

  15. Folds and Etudes (United States)

    Bean, Robert


    In this article, the author talks about "Folds" and "Etudes" which are images derived from anonymous typing exercises that he found in a used copy of "Touch Typing Made Simple". "Etudes" refers to the musical tradition of studies for a solo instrument, which is a typewriter. Typing exercises are repetitive attempts to type words and phrases…

  16. Experimental study of heat transfer for parallel flow in tube bundles with constant heat flux and for medium Prandtl numbers; Etude experimentale du transfert de chaleur dans des faisceaux tubulaires en ecoulement parallele pour une densite de flux thermique constante dans le domaine des nombres de Prandtl moyens

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    Rieger, M. [Commissariat a l' Energie Atomique, 91 - Saclay (France). Centre d' Etudes Nucleaires


    The heat transfer parameters were determined experimentally in electrically heated tube bundles for turbulent flow parallel to the axis. The tubes were arranged in a pattern of equilateral triangles. The ratios of the distance between the axes of the tubes to their external diameter were 1.60 and 1.25 in the two test sections studied. The experiments were carried out with distilled water and with a mixture of 60 per cent ethylene glycol and 40 per cent water. The values obtained for the Prandtl numbers in this way fell within the range from 2.3 to 18. The Reynolds numbers were varied between 10{sup 4} and 2.10{sup 5}. The relation between the mean heat transfer coefficients and the friction factor in the tube bundles was found from the experiments as: Nu = [Re Pr {zeta}/8]/[1+{radical}({zeta}/8) 8.8 (Pr-1.3) Pr{sup -0.22}]. The experimentally determined mean Nusselt numbers were also given by the following function: Nu = (0.0122 + 0.00245 p/d) Re{sup 0.86} Pr{sup 0.4}, with a maximum deviation of {+-}4 per cent. For certain local Nusselt numbers, deviations of up to 20 per cent with respect to the relations given were observed. (author) [French] Dans des faisceaux tubulaires a chauffage electrique parcourus par un ecoulement turbulent parallele a l'axe, on a determine experimentalement les parametres du transfert de chaleur. Les centres des sections droites des tubes etaient des sommets de triangles equilateraux. Les rapports de la distance a l'axe des tubes et leur diametre exterieur dans les deux veines de mesure etudiees etaient de 1.60 et 1.25. Des essais furent effectues avec de l'eau distillee ainsi qu'avec un melange de 60 pour cent de glycol ethylenique et 40 pour cent d'eau. Les valeurs des nombres de Prandtl obtenues ainsi etaient situees entre 2.3 a 18. On a fait varier les nombres de Reynolds entre 10{sup 4} et 2.10{sup 5}. La relation entre les nombres caracteristiques de transfert de chaleur moyens et la perte de charge dans

  17. Theoretical and experimental study of the bio-geochemical behaviour of americium 241 in simplified rhizosphere conditions. Application to a calcareous agricultural soil; Etude theorique et experimentale du comportement biogeochimique de l'americium-241 en conditions rhizospheriques simplifiees. Application dans un sol agricole calcaire

    Energy Technology Data Exchange (ETDEWEB)

    Perrier, T


    Americium 241, is one of the most radio-toxic contaminant produced during the nuclear fuel cycle. It can be found in all environmental compartments, in particular the soils. The main goals of this study are to identify, quantify and model the effect of the main factors controlling the mobility of {sup 241}Am in the rhizosphere and the agricultural soils. The physico-chemical parameters of the soil and of the soil solution, the potential role of microorganisms on the sorption-desorption processes, and the speciation of americium in solution have been more particularly studied. {sup 241}Am remobilization has been studied at the laboratory using leaching experiments performed in controlled conditions on reworked calcareous soils artificially contaminated with {sup 241}Am. The soil samples have been washed out in different hydrodynamic conditions by solutions with various compositions. The eluted solution has been analyzed (pH, conductivity, ionic composition, Fe{sub tot}, organic acids, {sup 241}Am) and its bacterial biomass content too. The overall results indicate that {sup 241}Am remobilization is contrasted and strongly linked with the condition under study (pH, ionic strength, glucose and/or citrate concentration). Therefore, a solution in equilibrium with the soil or containing small exudate concentrations (10{sup -4} M) re-mobilizes only a very small part of the americium fixed on the solid phase. The desorption of {sup 241}Am corresponds to a solid/liquid coefficient of partition (K{sub d}) of about 10{sup 5}{sup -1}. A significant addition of glucose induces an important dissolution of soil carbonates by the indirect action of microorganisms, but does not significantly favor the {sup 241}Am remobilization. On the other hand, the presence of strong citrate concentrations ({>=} 10{sup -2} M) allows 300 to 10000 time greater re-mobilizations by the complexing of {sup 241}Am released after the dissolution of the carrying phases. Finally, the colloidal






  19. Numerical and experimental investigation of industrial electrostatic precipitators; Etude numerique et experimentale d`electrofiltres industriels

    Energy Technology Data Exchange (ETDEWEB)

    Tochon, P.


    This work deals with electrostatic precipitators or ESP used for gas-solid particles separation. By means of a dust-controlled testing loop created and realised at the GRETh`s plate-form (Research Group on Heat Exchangers) and a numerical model developed during this work from TRIO software, the study of the performances of different ESP geometries has been carried out. Many electrical, hydraulic and particular parameters governing solid particles collection under ionised electric field have been identified, measured and modelled. The numerical model, ratified with experimental data obtained during this study and from literature, allows to describe local and global phenomena occurring in any geometries. Furthermore, parametric studies have been carried out in order to propose some optimised geometries. allowing to increase collection efficiencies. At least, on-site measurements with CETIAT (Centre Technique des Industries Aerauliques et Thermiques) allow to identify dust particles likely to be thrown out to the atmosphere, and troubles peculiar to large scales industrial plants. The numerical model has also been tested on these data. At the end of this study, an efficient dust-controlled experimental tool, PACIFIC loop, and a numerical simulation allowing ESP sizing are available. (author)

  20. Experimental study of the Rapsodie protections; Etude experimentale des protections de rapsodie

    Energy Technology Data Exchange (ETDEWEB)

    Chapelet, M.; Edeline, J.; Lhiaubet, G. [Commissariat a l' Energie Atomique, Fontenay-aux-Roses (France). Centre d' Etudes Nucleaires


    This report gives details of the protection tests which have been carried out by the Protection Sub-Commission during the starting-up trials on the reactor Rapsodie. The main types of tests reported concern the flux measurements in the core, the reflectors, the sodium and the near biological protections, the radio-protection measurements in the premises, and the activation of the principal fluids. In the last part, the results obtained are analyzed and compared to the forecast calculations. (author) [French] Ce rapport rend compte des essais de protection qui ont ete effectues dans le cadre des essais de demarrage de RAPSODIE par la Sous Commission Protection. Les principaux types d'essais rapportes sont les mesures de flux dans le coeur, les reflecteurs, le sodium et les protections biologiques proches, les mesures de radioprotection dans les locaux, et l'activation des principaux fluides. Dans la derniere partie, les resultats obtenus sont analyses et compares aux calculs previsionnels. (auteur)



    Catheline, Stefan


    Le premier chapitre de ce mémoire porte sur l'élastographie transitoire, une nouvelle technique ultrasonore capable de cartographier l'élasticité de cisaillement du corps humain. Cette information est utilisée depuis longtemps par les médecins lorsqu'ils procèdent à un examen par palpation. Un outil de palpation ultrasonore pourrait être extrêmement efficace pour dépister ou diagnostiquer certains cancers comme celui du sein par exemple. C'est le sujet de la première partie. La thèse de Laure...

  2. Etude aerodynamique d'un jet turbulent impactant une paroi concave (United States)

    LeBlanc, Benoit

    Etant donne la demande croissante de temperatures elevees dans des chambres de combustion de systemes de propulsions en aerospatiale (turbomoteurs, moteur a reaction, etc.), l'interet dans le refroidissement par jets impactant s'est vu croitre. Le refroidissement des aubes de turbine permet une augmentation de temperature de combustion, ce qui se traduit en une augmentation de l'efficacite de combustion et donc une meilleure economie de carburant. Le transfert de chaleur dans les au bages est influence par les aspects aerodynamiques du refroidissement a jet, particulierement dans le cas d'ecoulements turbulents. Un manque de comprehension de l'aerodynamique a l'interieur de ces espaces confinees peut mener a des changements de transfert thermique qui sont inattendus, ce qui augmente le risque de fluage. Il est donc d'interet pour l'industrie aerospatiale et l'academie de poursuivre la recherche dans l'aerodynamique des jets turbulents impactant les parois courbes. Les jets impactant les surfaces courbes ont deja fait l'objet de nombreuses etudes. Par contre des conditions oscillatoires observees en laboratoire se sont averees difficiles a reproduire en numerique, puisque les structures d'ecoulements impactants des parois concaves sont fortement dependantes de la turbulence et des effets instationnaires. Une etude experimentale fut realisee a l'institut PPRIME a l'Universite de Poitiers afin d'observer le phenomene d'oscillation dans le jet. Une serie d'essais ont verifie les conditions d'ecoulement laminaires et turbulentes, toutefois le cout des essais experimentaux a seulement permis d'avoir un apercu du phenomene global. Une deuxieme serie d'essais fut realisee numeriquement a l'Universite de Moncton avec l'outil OpenFOAM pour des conditions d'ecoulement laminaire et bidimensionnel. Cette etude a donc comme but de poursuivre l'enquete de l'aerodynamique oscillatoire des jets impactant des parois courbes, mais pour un regime d'ecoulement transitoire, turbulent

  3. Modelisation numerique et validation experimentale d'un systeme de protection contre le givre par elements piezoelectriques (United States)

    Harvey, Derek

    Le degivrage au moyen d'actuateurs piezoelectriques est considere comme une avenue prometteuse pour le developpement de systemes a faible consommation d'energie applicables aux helicopteres legers. Ce type de systeme excite des frequences de resonances d'une structure pour produire des deformations suffisantes pour rompre l'adherence de la glace. Par contre, la conception de tel systeme demeure generalement mal comprise. Ce projet de maitrise etudie l'utilisation de methodes numeriques pour assister la conception des systemes de protection contre le givre a base d'elements piezoelectriques. La methodologie retenue pour ce projet a ete de modeliser differentes structures simples et de simuler l'excitation harmonique des frequences de resonance au moyen d'actuateurs piezoelectriques. Le calcul des frequences de resonances ainsi que la simulation de leur excitation a ensuite ete validee a l'aide de montages experimentaux. La procedure a ete realisee pour une poutre en porte-a-faux et pour une plaque plane a l'aide du logiciel de calcul par elements finis, Abaqus. De plus, le modele de la plaque plane a ete utilise afin de realiser une etude parametrique portant sur le positionnement des actuateurs, l'effet de la rigidite ainsi que de l'epaisseur de la plaque. Finalement, la plaque plane a ete degivree en chambre climatique. Des cas de degivrage ont ete simules numeriquement afin d'etudier la possibilite d'utiliser un critere base sur la deformation pour predire le succes du systeme. La validation experimentale a confirme la capacite du logiciel a calculer precisement a la fois les frequences et les modes de resonance d'une structure et a simuler leur excitation par des actuateurs piezoelectriques. L'etude revele que la definition de l'amortissement dans le modele numerique est essentiel pour l'obtention de resultats precis. Les resultats de l'etude parametrique ont demontre l'importance de minimiser l'epaisseur et la rigidite afin de reduire la valeur des frequences

  4. El estudio de Trichinella spiralis en modelos experimentales.


    Alejandra Moreno G; Claudia Maldonado T; Isabel Chávez Ruvalcaba; Rosa G Reveles H; Zuleika Quiroz Núñez; José J. Muñoz E


    ResumenSe presenta una breve descripción del estudio de Trichinella spiralis en modelos experimentales, enfocándonos al estudio de la epidemiología, técnicas diagnósticas directas e indirectas, tratamiento y biología del parasito.

  5. Diseños cuasi-experimentales y longitudinales


    Bono Cabré, Roser


    Los diseños cuasi-experimentales son estrategias que, guiadas por unos objetivos, pretenden examinar las relaciones existentes entre una o más variables independientes y la variable dependiente o de respuesta. En el diseño cuasi-experimental, dado que los grupos son naturales, la equivalencia inicial se garantiza mediante la selección de grupos comparables o a través de alguna técnica de ajuste estadístico. Con los diseños longitudinales el investigador incorpora, dentro de la estructura del ...

  6. An experimental study of mixed convection; Contribution a l'etude experimentale de la convection mixte

    Energy Technology Data Exchange (ETDEWEB)

    Saez, M.


    The aim of our study is to establish a reliable database for improving thermal hydraulic codes, in the field of turbulent flows with buoyancy forces. The flow considered is mixed convection in the Reynolds and Richardson number range: Re = 10{sup 3} to 6.10{sup 4} and Ri = 10{sup -4} to 1. Experiments are carried out in an upward turbulent flow between vertical parallel plates at different wall temperatures. Part 1 gives a detailed database of turbulent mixed flow of free and forced convection. Part 2 presents the installation and the calibration system intended for probes calibration. Part 3 describes the measurement technique (constant temperature probe and cold-wire probe) and the method for measuring the position of the hot-wire anemometer from the wall surface. The measurement accuracy is within 0.001 mm in the present system. Part 4 relates the development of a method for near wall measurements. This correction procedure for hot-wire anemometer close to wall has been derived on the basis of a two-dimensional numerical study. The method permits to obtain a quantitative correction of the wall influence on hot-wires and takes into account the velocity profile and the effects the wall material has on the heat loss. Part 5 presents the experimental data obtained in the channel in forced and mixed convection. Results obtained in the forced convection regime serve as a verification of the measurement technique close to the wall and give the conditions at the entrance of the test section. The effects of the buoyancy force on the mean velocity and temperature profiles are confirmed. The buoyancy strongly affects the fluid structure and deforms the distribution of mean velocity. The velocity profiles are asymmetric. The second section of part 5 gives an approach of analytical wall functions with buoyancy forces, on the basis of the experimental data obtained in the test section. (author)

  7. Experimental study of drop breakup in a turbulent flow; Etude experimentale de la rupture de gouttes dans un ecoulement turbulent

    Energy Technology Data Exchange (ETDEWEB)

    Galinat, S.


    This work presents the drop breakup phenomenon in a turbulent flow induced by a cross-section restriction in a pipe. A global analysis of single-drop breakup, in a finite volume downstream of the orifice, has allowed deriving statistical quantities such as the break-up probability and the daughter-drop distribution. These parameters are function of a global Weber number based on the maximal pressure drop through the orifice. At a local scale, the locations of breakup events are distributed heterogeneously and depend on the flow Reynolds number. The local hydrodynamic study in downstream of the orifice, which has been done by using Particle Image Velocimetry, reveals the specific breakup zones. Otherwise, this analysis has proved that the turbulence is the predominant external stress at the drop scale. The relation between drop deformation and the external stress along the trajectory has been simulated numerically by the response of a damped oscillator to the locally measured instantaneous turbulence forcing. The results of statistical analysis have allowed to introduce a breakup criterion, based on a unique deformation threshold value for all experiments. This multi-scale approach has been conducted to study drop breakup mechanisms in a concentrated dispersion. The breakup probability decrease with the increase of dispersed phase concentration, which influences the turbulent Weber number distribution in downstream of the orifice. (author)

  8. Etude de la transmission sonore a travers un protecteur de type "coquilles" : modelisation numerique et validation experimentale (United States)

    Boyer, Sylvain

    On estime que sur les 3,7 millions des travailleurs au Quebec, plus de 500 000 sont exposes quotidiennement a des niveaux de bruits pouvant causer des lesions de l'appareil auditif. Lorsqu'il n'est pas possible de diminuer le niveau de bruit environnant, en modifiant les sources de bruits, ou en limitant la propagation du son, le port de protecteurs auditifs individualises, telles que les coquilles, demeure l'ultime solution. Bien que vue comme une solution a court terme, elle est communement employee, du fait de son caractere peu dispendieux, de sa facilite d'implantation et de son adaptabilite a la plupart des operations en environnement bruyant. Cependant les protecteurs auditifs peuvent etre a la fois inadaptes aux travailleurs et a leur environnement et inconfortables ce qui limite leur temps de port, reduisant leur protection effective. Afin de palier a ces difficultes, un projet de recherche sur la protection auditive intitule : " Developpement d'outils et de methodes pour ameliorer et mieux evaluer la protection auditive individuelle des travailleur ", a ete mis sur pied en 2010, associant l'Ecole de technologie superieure (ETS) et l'Institut de recherche Robert-Sauve en sante et en securite du travail (IRSST). S'inscrivant dans ce programme de recherche, le present travail de doctorat s'interesse specifiquement a la protection auditive au moyen de protecteurs auditifs " passifs " de type coquille, dont l'usage presente trois problematiques specifiques presentees dans les paragraphes suivants. La premiere problematique specifique concerne l'inconfort cause par exemple par la pression statique induite par la force de serrage de l'arceau, qui peut reduire le temps de port recommande pour limiter l'exposition au bruit. Il convient alors de pouvoir donner a l'utilisateur un protecteur confortable, adapte a son environnement de travail et a son activite. La seconde problematique specifique est l'evaluation de la protection reelle apportee par le protecteur. La methode des seuils auditifs REAT (Real Ear Attenuation Threshold) aussi vu comme un "golden standard" est utilise pour quantifier la reduction du bruit mais surestime generalement la performance des protecteurs. Les techniques de mesure terrains, telles que la F-MIRE (Field Measurement in Real Ear) peuvent etre a l'avenir de meilleurs outils pour evaluer l'attenuation individuelle. Si ces techniques existent pour des bouchons d'oreilles, elles doivent etre adaptees et ameliorees pour le cas des coquilles, en determinant l'emplacement optimal des capteurs acoustiques et les facteurs de compensation individuels qui lient la mesure microphonique a la mesure qui aurait ete prise au tympan. La troisieme problematique specifique est l'optimisation de l'attenuation des coquilles pour les adapter a l'individu et a son environnement de travail. En effet, le design des coquilles est generalement base sur des concepts empiriques et des methodes essais/erreurs sur des prototypes. La piste des outils predictifs a ete tres peu etudiee jusqu'a present et meriterait d'etre approfondie. L'utilisation du prototypage virtuel, permettrait a la fois d'optimiser le design avant production, d'accelerer la phase de developpement produit et d'en reduire les couts. L'objectif general de cette these est de repondre a ces differentes problematiques par le developpement d'un modele de l'attenuation sonore d'un protecteur auditif de type coquille. A cause de la complexite de la geometrie de ces protecteurs, la methode principale de modelisation retenue a priori est la methode des elements finis (FEM). Pour atteindre cet objectif general, trois objectifs specifiques ont ete etablis et sont presentes dans les trois paragraphes suivants. (Abstract shortened by ProQuest.).

  9. Experimental contribution to the study of gas bearings; Contribution experimentale a l'etude des paliers a gaz

    Energy Technology Data Exchange (ETDEWEB)

    Gobert, G


    Developments for gas-lubricated bearings are discussed. The study of how the fluid film behaves compared to what one can expect is discussed. Various devices are described which have allowed us to go up to peripheral velocity greater than 400 m/s, leading to rotational speed of several thousand revolutions per second. This is obtained by using an automatic setting for the distance between fixed and rotating walls. (author) [French] Le present travail debute par un apercu de l'etat actuel des connaissances sur le processus de la lubrification, notamment sur les paliers a lubrification par gaz. Apres une analyse effectuee au moyen des recentes theories de R. Comolet sur le film fluide entre disques paralleles, nous decrivons des machines dont les charges axiales et radiales specialement etudiees ont permis, au moyen d'un reglage automatique des jeux entre parois fixes et parois tournantes, d'atteindre des vitesses peripheriques superieures a 400 m/s correspondant a des vitesses de plusieurs milliers de tours par seconde. (auteur)

  10. Experimental study of a water thermo-capillary loop; Etude experimentale d`une boucle thermocapillaire a eau

    Energy Technology Data Exchange (ETDEWEB)

    Lefriec, C.; Alexandre, A. [Ecole Nationale Superieure de Mecanique et d`Aerotechnique (ENSMA), 86 - Poitiers (France)


    This paper presents a bench scale experiment of a water thermo-capillary loop which allows to improve the understanding of its functioning mechanisms thanks to the internal visualisation of each component using transparent walls. The advantages of water are its non-toxicity, its high chemical compatibility with several materials and its low functioning pressure. The experimental device is presented and the functioning regimes of each component is analyzed: condenser (flow visualization, influence of tilt), evaporator (quality of heat exchange between teeth and porous medium, bubbles, heat exchange coefficient). (J.S.)

  11. Etude des etats electroniques en champ magnetique dans le niveau de Landau N=0 de la tricouche ABC de graphene (United States)

    Rondeau, Maxime

    Dans cet ouvrage nous etudions les phases du gaz d'electrons bidimensionnel dans la tricouche de graphene en empilement ABC. En partant du modele des liaisons fortes et en faisant l'approximation du continuum autour des vallees K +, K-, nous obtenons un modele effectif a deux bandes qui permet de decrire la physique de basse energie des electrons en champ magnetique dans cette structure. Ce modele contient trois orbitales degenerees dans le niveau de Landau N = O. Ce dernier est donc 12N φ, fois degeneres en incluant les degres de liberte de spin et de vallee. En ajoutant l'interaction de Coulomb au systeme et en considerant seulement les remplissages v = -5, -4, -4, 5 afin d'avoir un systeme a trois niveaux, nous etudions le diagramme de phase du gaz d'electrons en fonction d'un biais electrique entre les couches externes. Nous trouvons une phase d'onde de densite de charge bidimensionnelle (ODC2D) comme etat fondamental du systeme. Cette ODC2D se nomme cristal dans ce memoire et nous derivons ses proprietes de transports et ses modes collectifs. Nous discutons egalement du caractere topologique de ce cristal. Notre etude englobe aussi les phases liquides avec ou sans coherence orbitale. Nous concluons notre memoire par l'etude de quelques signatures experimentales des phases du gaz d'electrons dans la tricouche.

  12. Conservation of an ion beam brightness. Study of a non brightness disturbing lens; Conservation de la brillance d'un faisceau ionique. Etude d'une lentille ne deteriorant pas cette brillance

    Energy Technology Data Exchange (ETDEWEB)

    Bernard, P. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    Experimental studies of ion sources prove that large initial brightnesses can be obtained by using the plasma expansion principle. However these brightnesses are usually spoiled by the beam focusing and accelerating systems. A high intensity focusing set up is first theoretically studied, then numerically determined by use of a 7094 IBM computer. Aberrations have been minimized. It has then been possible to construct a set up conserving the source initial brightness. For a 100 mA beam the focusing voltage is 150 kV, the beam study has been done for 350 keV beam final energy. Given is a discussion of results. (author) [French] L'etude experimentale des sources d'ions, montre que de grandes brillances initiales peuvent etre atteintes en utilisant le principe de l'expansion du plasma. Mais generalement ces brillances sont alterees par la focalisation et l'acceleration du faisceau. Nous presentions une etude theorique, suivie d'une determination numerique utilisant l'ordinateur IBM 7094, d'un montage capable de focaliser des intensites elevees. Nous nous efforcons de reduire au minimum les aberrations. Nous avons pu realiser un systeme conservant la brillance initiale de la source. Pour une intensite de 100 mA la tension de focalisation doit etre de 150 kV. L'etude du faisceau a ete faite a 350 keV. Nous discutons enfin des resultats obtenus. (auteur)

  13. Study of the interactions bacteria - phenanthrene - activated carbon for the preparation of a permeable reactive barrier; Etude des interactions bacteries - phenanthrene - charbon en vue de l'elaboration d'une barriere permeable reactive

    Energy Technology Data Exchange (ETDEWEB)

    Leglize, P.


    Permeable Reactive Barrier (PRB) is a new way for the remediation of contaminated groundwater, but up to now Polycyclic Aromatic Hydrocarbons (PAHs) were rarely considered. We investigated PAH - bacteria - materials interactions in order to validate the feasibility of PRB for PAH contamination. PHE Adsorption/desorption kinetics onto different materials, activated carbons (CA), pozzolana (Pz) and pozzolana coated with heavy fuel (PzF), were investigated. PHE biodegradation were performed on batch using PAH degrading bacteria and the PRB materials. CA was a good media for PRB process: Phenanthrene sorption capacity is 100 to 10000 fold higher than PzF and Pz. Phenanthrene mineralization with CA was higher than without material. Bacterial properties affected PHE biodegradation. Bio-film production improved PHE biodegradation by PAH degrading bacteria. Column studies showed that inoculation of the column improved its efficiency: adsorbed PHE degradation and increased retardation of PHE. (author)

  14. Etude vibroacoustique d'un systeme coque-plancher-cavite avec application a un fuselage simplifie (United States)

    Missaoui, Jemai

    L'objectif de ce travail est de developper des modeles semi-analytiques pour etudier le comportement structural, acoustique et vibro-acoustique d'un systeme coque-plancher-cavite. La connection entre la coque et le plancher est assuree en utilisant le concept de rigidite artificielle. Ce concept de modelisation flexible facilite le choix des fonctions de decomposition du mouvement de chaque sous-structure. Les resultats issus de cette etude vont permettre la comprehension des phenomenes physiques de base rencontres dans une structure d'avion. Une approche integro-modale est developpee pour calculer les caracteristiques modales acoustiques. Elle utilise une discretisation de la cavite irreguliere en sous-cavites acoustiques dont les bases de developpement sont connues a priori. Cette approche, a caractere physique, presente l'avantage d'etre efficace et precise. La validite de celle-ci a ete demontree en utilisant des resultats disponibles dans la litterature. Un modele vibro-acoustique est developpe dans un but d'analyser et de comprendre les effets structuraux et acoustiques du plancher dans la configuration. La validite des resultats, en termes de resonance et de fonction de transfert, est verifiee a l'aide des mesures experimentales realisees au laboratoire.

  15. ETUDE - European Trade Union Distance Education. (United States)

    Creanor, Linda; Walker, Steve


    Describes transnational distance learning activities among European trade union educators carried out as part of the European Trade Union Distance Education (ETUDE) project, supported by the European Commission. Highlights include the context of international trade union distance education; tutor training course; tutors' experiences; and…

  16. Creating Pedagogical Etudes for Interactive Instruments


    Butler, Jennifer


    In this paper I discuss the importance of and need forpedagogical materials to support the development of newinterfaces and new instruments for electronic music. I describemy method for creating a graduated series of pedagogicaletudes composed using Max/MSP. The etudes will helpperformers and instrument designers learn the most commonlyused basic skills necessary to perform with interactiveelectronic music instruments. My intention is that the finalseries will guide a beginner from these init...

  17. Some aspects of nuclear graphite production in France; Etude generale sur les graphites nucleaires produits en France

    Energy Technology Data Exchange (ETDEWEB)

    Gueron, J.; Hering, H. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires; Legendre, A. [Pechiney, 75 - Paris (France)


    1) Manufacturing: A summary and results on the CEA-Pechiney purification process are given. Variations in the preparation of green pastes and their effects on graphitized material are described. 2) Physical and mechanical properties: Results are given on: - Statistics of dimensional variatior products having square cross-section. - Statistical variation of thermal expansion coefficients and of electrical conductivity. - Density of normals to carbon layer planes and their connexion with thermal expansion. - Stress-strain cycles and conclusions drawn therefrom. - Mechanical resistance and gas permeability of items for supporting fuel elements. 3) Behaviour under radiation: Alteration under radiation of French graphites irradiated either in G1 pile or in experimental piles, and thermal annealing of those alterations, are given. (author)Fren. [French] 1) Fabrication: On resume le procede d'epuration CEA-PECHINEY, ainsi que diverses modalites de preparation des pates et on expose les resultats obtenus. 2) Proprietes physiques et mecaniques: On indique le resultat d'etudes sur: - la statistique des dimensions de produits a section carree. - celle des variations des coefficients de dilatation thermique et de la conductibilite electrique. - la densite des normales aux plans graphitiques et leur connexion avec la dilatation thermique. - la compression mecanique du graphite. - la solidite mecanique et la permeabilite aux gaz de pieces destinees a supporter des cartouches de combustible. 3) Tenue sous rayonnement: Modification sous rayonnement des graphites fran is irradies soit dans la pile G1, soit dans des piles experimentales, et guerison thermique de ces modifications. (auteur)

  18. Etude des comportements rheologiques des melanges de farine ble ...

    African Journals Online (AJOL)

    Etude des comportements rheologiques des melanges de farine ble/sorgho sans tanins issue de trois nouvelles varietes cultivees au Senegal et mise au point de pains a base de farines composees (ble/sorgho)

  19. Instrumental performance of an etude after three methods of practice. (United States)

    Vanden Ark, S


    For 80 fifth-grade students three practice conditions (mental, mental with physical simulation, and physical with singing) produced significant mean differences in instrumental performance of an etude. No significant differences were found for traditional, physical practice.


    Directory of Open Access Journals (Sweden)

    Eliexer Pérez Lozada


    Full Text Available Se presenta un conjunto de prototipos experimentales para el aprendizaje de la óptica. Los prototipos fueron elaborados con materiales de bajo costo y de fácil adquisición, mejorados en sus diseños, validados por expertos y probados en el aula para su valoración. Con ellos se recreó una metodología sustentada en constructos y procesos que mejoró el logro de los alumnos, mostrando su pertinencia y efectividad para la enseñanza de la óptica.


    Directory of Open Access Journals (Sweden)

    Pérez Lozada, Eliexer


    Full Text Available Se presenta un conjunto de prototipos experimentales para el aprendizaje de la óptica. Los prototipos fueron elaborados con materiales de bajo costo y de fácil adquisición, mejorados en sus diseños, validados por expertos y probados en el aula para su valoración. Con ellos se recreó una metodología sustentada en constructos y procesos que mejoró el logro de los alumnos, mostrando su pertinencia y efectividad para la enseñanza de la óptica

  2. Ensayos Experimentales del Efecto Magnus sobre diferentes cuerpos cilíndricos


    Pezzoti, S.; Nadal Mora, Vicente J.; Sanz Andres, Angel Pedro


    La presente publicación presenta el desarrollo de diferentes ensayos experimentales con el fin de poder determinar las características aerodinámicas asociadas al Efecto Magnus en cuerpos no cilíndricos circulares. Se realiza la descripción del banco y los equipos utilizados para los ensayos, los modelos, la metodología y los resultados de los ensayos realizados. Se presentan la sustentación y resistencia aerodinámica de los modelos para diferentes velocidades de rotación y de la corriente de ...

  3. Adsorption of trace elements on a bentonite sample: experimental study and modelling; Retention d'elements trace sur une bentonite: etude experimentale et modelisation

    Energy Technology Data Exchange (ETDEWEB)

    Hurel, Ch


    Radioactive waste storage in deep underground is studied in France. The alteration of the radioactive waste can mobilize the elements that can migrate to the geosphere. Clays (bentonite) are investigated as major materials constituting the barriers because of their low permeability and high retention capacity. A thermodynamic surface complexation model, based on the component additivity principle was investigated, in order to predict the adsorption behaviour of pollutants in contact with clay. This principle allows an independent determination of each parameter of the model, limiting the fitting procedure of correlated data, and it allows the use of an adsorption thermodynamic database for a solid. The predictions obtained were in good accordance with experimental data. The model could then be applied successfully in performance assessment. (author)

  4. Experimental study and modelling of iron ore reduction by hydrogen; Etude experimentale et modelisation de la reduction du minerai de fer par l'hydrogene

    Energy Technology Data Exchange (ETDEWEB)

    Wagner, D


    In an effort to find new ways to drastically reduce the CO{sub 2} emissions from the steel industry (ULCOS project), the reduction of iron ore by pure hydrogen in a shaft furnace was investigated. The work consisted of literature, experimental, and modelling studies. The chemical reaction and its kinetics were analysed on the basis of thermogravimetric experiments and physicochemical characterizations of partially reduced samples. A specific kinetic model was designed, which simulates the successive reactions, the different steps of mass transport, and possible iron sintering, at the particle scale. Finally, a 2-dimensional numerical model of a shaft furnace was developed. It depicts the variation of the solid and gas temperatures and compositions throughout the reactor. One original feature of the model is using the law of additive characteristic times for calculating the reaction rates. This allowed us to handle both the particle and the reactor scale, while keeping reasonable calculation time. From the simulation results, the influence of the process parameters was assessed. Optimal operating conditions were concluded, which reveal the efficiency of the hydrogen process. (author)

  5. The transition radiation. 2. experimental study of the optical transition radiation; Le rayonnement de transition: 2. etude experimentale du rayonnement de transition optique

    Energy Technology Data Exchange (ETDEWEB)

    Couillaud, Ch.; Haouat, G.; Seguin, S.; Striby, S


    Optical-transition-radiation-based diagnostics have been widely used for many years on electron accelerators in order to measure beam energy and transverse and longitudinal emittances. These diagnostics are very attractive for high brightness electron beams used as drivers for radiation sources. Such diagnostics have been performed on the ELSA facility (18 MeV electron energy, 100 A peak current) using both a single interface and an OTR-Wartski interferometer. We present the accelerator, the experimental set-up and the method for analyzing the OTR angular distribution. Then, the experimental results are described and compared with those from the three gradient method. In addition, we present a beam energy measurement using OTR interferogram analysis. (author)

  6. Etude experimentale de la photoexcitation des colorants de type anthracene, azobenzene et thioindigo dans des matrices de cristaux liquides nematiques et smectiques (United States)

    Saad, Bendaoud

    Nous avons étudié le comportement de différents colorants (tétracène et azobenzènes AZD3, DR1 et D2) dans des matrices de cristaux liquides (E7 et 5CB). Les résultats obtenus ont montré que la réorientation optique des molécules de cristaux liquides se produit à des intensités extrêmement faibles (~1 μW/cm 2). Nous avons attribué cet effet à l'interaction entre les molécules des colorants photoexcitées et celles des cristaux liquides hôtes. Ces interactions se produisent lorsque les molécules dichroïques exçitées transitent entre des états triplets intermédiaires tout en conduisant à la génération d'un nouveau moment optique géant, responsable de la réorientation des molécules. Le changement de la forme de la molécule du colorant, dû à l'isomérisation Trans-Cis , est à l'origine de la création de ce moment. Ce qui distingue notre réorientation moléculaire de celle obtenue sur d'autres systèmes hôtes-dopants est que les molécules sont repoussées du champ électrique de la lumière. Par ailleurs, cette réorientation est accompagnée d'une diffusion anisotrope des molécules excitées. Nous avons aussi étudié le cristal liquide PhBz rendu ferroélectrique en le dopant par des molécules thioindigos. En exploitant l'augmentation de la polarisation spontanée due à la photoisomérisation du colorant, nous avons pu réaliser un hologramme dynamique contrôlable par un champ optique assisté par une tension statique.

  7. Lanthanides in geological fluids: experimental study of standard thermodynamic properties and of solubilities; Les lanthanides dans les fluides geologiques: etude experimentale des proprietes thermodynamiques standard et des solubilites

    Energy Technology Data Exchange (ETDEWEB)

    Pourtier, E


    Standard thermodynamic properties (STP) of lanthanides (Ln{sup 3+}) are necessary to predict their transport in hydrothermal fluids. New STP (apparent molal volumes and heat capacities) of Ln{sup 3+} are determined with dilute (La{sup 3+}, Nd{sup 3+}, Gd{sup 3+}, Yb{sup 3+}) triflates solutions, up to 300 deg. C and 300 bars, using a vibrating tube flow densimeter and a differential heat flow calorimeter. The triflate anion (CF{sub 3}SO{sub 3}), stable at high temperature, does not form complexes with Ln{sup 3+}. The STP of HCF{sub 3}SO{sub 3} and NaCF{sub 3}SO{sub 3} are measured in order to get the STP of CF{sub 3}SO{sub 3}. The solubility of the Nd-pure pole of monazite (NdPO{sub 4}) studied between 300 and 800 deg. C at 2 kbars in H{sub 2}O and H{sub 2}O+NaCl using weight loss and isotope dilution methods, is prograde for neutral pH. The study of Nd{sup 3+} speciation at 650 deg. C and 300 deg. C, 2 kbars, shows that only hydroxylated species are present. These data allow the revision of Ln{sup 3+} parameters in the HKF model. (author)

  8. Experimental and theoretical study of the atmospheric boundary layer over the paris area; Etude experimentale et theorique de la couche limite atmospherique en agglomeration parisienne

    Energy Technology Data Exchange (ETDEWEB)

    Menut, L


    This thesis studied the urban boundary layer dynamic behaviour over the Paris area by comparing urban (Paris) and suburban (Palaiseau) dynamic data such as lidars, sodars, sonic anemometers. All the data were obtained during the ECLAP experiment, specifically performed to characterize the differences between a city and its near environment. (author)

  9. Experimental study of heat and mass transfer across a density interface; Etude experimentale des transferts de chaleur et de masse a travers une interface de densite

    Energy Technology Data Exchange (ETDEWEB)

    Zellouf, Y.


    This work is part of a major research program on stratified flows encountered in the storage tanks of liquid natural gas. The subject of this dissertation concerns more particularly the problem of heat and mass transfer across a density interface. These flows are complex by nature due to the combination of three parameters: density difference {delta}{rho} across the interface, turbulence intensity u' in the homogeneous layers and the diffusivity K of the stratifying components. To control the operational conditions of the liquid gas storage, it is crucial to have a universal physical model to estimate the fluxes across the interface and to predict the brutal mixing of homogeneous layers commonly called the 'roll-over' event. In this work heat and mass transfer across an interface is measured in the presence of turbulence generated by vertical oscillation of two grids. Turbulent velocity field is measured in a homogeneous environment by Particle Image velocimetry (PIV). It shows that the turbulence generated by the two grids is homogeneous and isotropic in the vertical plan. We have established, based on this measurements, an experimental decay law of the turbulence intensity as a function of the flow parameters and the distance from the grids. Stratifications have been followed in time by recording the vertical profiles of temperature and density. Measured heat and mass fluxes are presented as a function of the three flow parameters (u', {delta}{rho}, K) through the non-dimensional numbers: Richardson number Ri g({delta}{rho}/{rho}{sub 0})(l'/u'{sup 2}) and Peclet number Pe = u'l'/K. Measurements show that these two parameters have opposite effects on transfer phenomena. While thermal diffusivity favours the entrainment near the interface, the stratification effect limits this entrainment. This fact is explained by analysing the interface structure. A phenomenological model is then proposed which takes into account these parameters. (author)

  10. Etude experimentale et optimisation d'un systeme hybride hydraulique pour camions a ordures et amelioration des performances par raffinement de sa logique de controle (United States)

    Lacroix, Benoit

    The aim of this thesis is to demonstrate experimentally the operation of a hydraulic hybrid system specifically dedicated to the application of refuse trucks in addition to proposing solutions to improve its control strategy. The developed hybrid system recovers the vehicle's kinetic energy during braking. A variable displacement hydraulic motor then uses the energy stored in a hydraulic accumulator to assist the internal combustion engine (ICE) at suitable times. The particular aspect of this system is that assistance to the ICE can occur when it operates at idle and drives the auxiliary hydraulic equipment of the refuse truck. Essentially, the control strategy initially developed maximizes the recovery of braking energy and uses that energy to minimize the solicitation of the ICE at idle. The experimental results obtained with two prototypes tested in real operating conditions show that the hybrid system can recover a significant portion of braking energy. In addition, the results show that it is possible to reduce the load on the ICE during idle with the application of an assisting torque. However, the advantage of assisting the ICE in specific areas of the operating range is slim since the ICE's gross efficiency varies only slightly depending on conditions of operation. This is confirmed by the optimization of the control logic using deterministic dynamic programming. Indeed, by managing the pressure in the accumulator to maximize the amount of energy recovered during braking and by dosing the assistance to the ICE in an ideal fashion, the optimal control only managed to improve fuel savings by 6% in comparison to the original control. Therefore, since the efforts that would be required to emulate the ideal behavior in real time are significant for a relatively small and uncertain gain, the initial control logic is considered near optimal. Finally, this thesis proposes an improved version of the torque assisting hybrid system that could shut down the ICE when the vehicle is stopped while maintaining functional the auxiliary hydraulic equipment. An optimization of the control logic indicates that proper management of the pressure in the accumulator would allow turning off the ICE most of the time at stop and thus, would increase the fuel savings by over 40% compared to the original system. The simulation of a basic control strategy shows that such pressure management may be feasible in real time and that the potential gain in fuel savings is achievable. Keywords: hybrid system, hydraulic, control, refuse truck.

  11. Experimental study of the topological aspect of the ergodic divertor in Tore-supra tokamak; Etude experimentale des aspects topologiques du divertor ergodique de Tore Supra

    Energy Technology Data Exchange (ETDEWEB)

    Costanzo, L


    The control of power deposition onto plasma facing components in tokamaks is a determining factor for future thermonuclear fusion reactors. Plasma surface interaction can be performed using limiters or divertors. The ergodic divertor installed on Tore Supra is an atypical example of a magnetic divertor. It consists in applying a magnetic perturbation which establishes a particular topology of the plasma in contact with the wall (edge plasma). We carried out dedicated experiments in order to study parallel heat flux which strike the divertor neutralizers. This quantitative and qualitative analysis of heat flux as a function of experimental conditions allows to determine the profiles of power deposition along the neutralizers. The influence of plasma electron density, additional heating, impurities and injected gas was established. An experimental study of the sheath heat transmission factor {gamma} was carried out by correlating measurements made with Langmuir probes and infrared imaging. This study gave rise to a major conclusion: for ohmic discharges with deuterium injection and most of the time with helium, it was experimentally confirmed that {gamma}=7 in agreement with classical sheath theory. However, an increase of this factor with additional power has been shown. Detached plasma, which is an attractive regime in order to reduce the power deposition, requires an optimized control. A new measurement of the detachment onset has been developed. It is based on the variation of heat flux onto the plates derived from infrared measurements. A detachment cartography with the determination of a new 2D 'IR' Degree of Detachment was carried out allowing to locate the zone where the detachment starts. We can apply this concept both to other tokamaks such as JET and ITER. A comparison between the axisymmetric divertor and the ergodic divertor is also presented concerning the power deposition in the two configurations. Low heat flux with the ergodic divertor is a major advantage. (author)

  12. Experimental and theoretical study of phase transitions under ball milling; Etude experimentale et modelisation des changements de phases sous broyage a haute energie

    Energy Technology Data Exchange (ETDEWEB)

    Pochet, P


    The aim of this work was to determine how phase transition s under ball-milling depend on the milling conditions and to find out if one can rationalize such transitions with the theory of driven alloys. We have chosen two phase transitions: the order-disorder transition in Fe Al and the precipitation-dissolution NiGe. In the case of Fe Al we have found that the steady-state long range order parameter achieved under ball milling intensity; moreover the same degree of order is achieved starting from an ordered alloy or a disordered solid solution. On the way to fully disordered state the degree of order either decreases monotonically or goes through a short lived transient state. This behaviour is reminiscent of a first order transition while the equilibrium transition is second order. All the above features are well reproduced by a simple model of driven alloys, which was originally build for alloys under irradiation. The stationary degree of order results of two competitive atomic jump mechanisms: the forced displacements induced by the shearing of the grains, and the thermally activated jumps caused by vacancies migrations. Finally we have performed atomistic simulations with a Monte Carlo kinetic algorithm, which revealed the role of the fluctuations in the intensity of the forcing. Moreover we have shown that specific atomistic mechanisms are active in a dilute NiGe solid solution which might lead to ball milling induced precipitation in under-saturated solid solution. (author). 149 refs.

  13. Multi scale experimental study of water and ionic transport in porous charged media: clays; Etude experimentale multiechelle du transport ionique et aqueux en milieu poreux charge: argiles

    Energy Technology Data Exchange (ETDEWEB)

    Cadene, A


    Clays are porous media of industrial interest. Due to their retention capacities and low permeability to water, they are the principal candidate for the conception of engineered barriers radioactive waste disposal. The main interest of this study is the experimental determination of the cationic and water dynamics in montmorillonite and fluoro-hectorite at low water contents This latter synthetic smectite has been used as a model clay to help the interpretation of the results issued from the first natural one. After a summary on the clayey system, this work reports the many experimental techniques (Atomic Force Microscopy, Photo-Correlation Spectroscopy, Micro-calorimetry, Powder Diffraction) used during the preliminary study concerning structural characterisation of the samples. The study of the sodic form of smectites with the use of a combination of quasi-elastic neutron scattering techniques (Time of Flight and Spin Echo) succeeded to water diffusion coefficients but also to a discernment of the limits of such techniques. Experiments with montmorillonite samples are in agreement with the simulations, so tending to a validation of the models. Experimental data obtained from synthetic hectorites will be in the near future compared to simulations In the last part, this work shows the application of Broad Band Dielectric Spectroscopy for the investigation of ionic dynamic in these porous media. Many models have been developed for the interpretation of the experimental raw data obtained with this technique. (author)

  14. An experimental study of electrical and dielectric properties of consolidated clayey materials; Etude experimentale des proprietes electriques et dielectriques des materiaux argileux consolides

    Energy Technology Data Exchange (ETDEWEB)

    Comparon, L


    This study is devoted to the electrical and dielectric properties of consolidated clays. A better understanding of the conduction and polarization phenomena in clays is necessary to better interpret in situ measurements in terms of water saturation and texture. An experimental study was carried out on synthetic clay samples (kaolinite and smectite) compacted with various water contents, porosities and mineralogical compositions, on a large frequency range, using three laboratory setups. The electrical properties of natural argillites (from ANDRA) were then investigated. We found that the response of the synthetic samples is mainly controlled by water content on the whole frequency range; two polarization phenomena were observed, which were related to the Maxwell-Wagner polarization and the electrical double layer polarization around the clay particles. The electrical response of argillites is more complex; it is controlled by water content but also by the microstructure of the rock. In these rocks, the electrical and dielectric anisotropies are high; anisotropy was also measured for the synthetic clays. The existing models explain the high frequency limit of the dielectric permittivity of the clayey materials, but the low frequency part of the spectra ({<=}1 MHz) needs theoretical developments. (author)


    Directory of Open Access Journals (Sweden)



    Full Text Available Dans cette étude nous présentons d’une part la structure cristalline du 1-iodo-2,3,5,6-tétraméthylebenzène (C10H13I aussi connu comme iododurène à partir de la diffraction des rayons X à température ambiante et d’autre part la conformation moléculaire de ce même composé à partir des calculs de la théorie de la fonctionnelle de la densité (DFT. Ce composé cristallise dans le système orthorhombique, groupe spatial P212121 et Z=4. L’empilement moléculaire se fait suivant le plus court axe cristallographique a. Les résultats de calcul de la mécanique quantique en utilisant la chaîne de programme Gaussian03 (DFT avec deux fonctionnelles B3LYP et MPW1PW91 et le jeu de base DGDZVP ont conduit à deux conformations différences  de symétrie C2v et Cs avec des énergies de formation minimales voisines. En prenant en compte les résultats de calcul de la fonctionnelle MPW1PW91/DGDZVP comparés à l'expérience on trouve que les écarts sont compris entre  ± 0.103° pour les angles de liaison  et ± 0.001 Å  pour les longueurs de liaison. Le programme permet également d’obtenir en  plus  du calcul d’optimisation de prédire les modes normaux de vibrations internes de l’iododurène. Ainsi, l’attribution des modes donne un très bon accord entre les fréquences calculées et observées.

  16. Thermal design of horizontal tube boilers. Numerical and experimental investigation; Modelisation thermique de bouilleurs a tubes horizontaux. Etude numerique et validation experimentale

    Energy Technology Data Exchange (ETDEWEB)

    Roser, R.


    This work concerns the thermal design of kettle reboilers. Current methods are highly inaccurate, regarded to the correlations for external heat transfer coefficient at one tube scale, as well as to two-phase flow modelling at boiler scale. The aim of this work is to improve these thermal design methods. It contains an experimental investigation with typical operating conditions of such equipment: an hydrocarbon (n-pentane) with low mass flux. This investigation has lead to characterize the local flow pattern through void fraction measurements and, from this, to develop correlations for void fraction, pressure drop and heat transfer coefficient. The approach is original, since the developed correlations are based on the liquid velocity at minimum cross section area between tubes, as variable characterizing the hydrodynamic effects on pressure drop and heat transfer coefficient. These correlations are shown to give much better results than those suggested up to now in the literature, which are empirical transpositions from methods developed for inside tube flows. Furthermore, the numerical code MC3D has been applied using the correlations developed in this work, leading to a modeling of the two-phase flow in the boiler, which is a significant progress compared to current simplified methods. (author)

  17. Contribution to the experimental study of the polarized liquid helium-3; Contributions a l'etude experimentale de l'helium-3 liquide polarise

    Energy Technology Data Exchange (ETDEWEB)

    Villard, B


    Spin-polarized liquid helium-3 is prepared by laser optical pumping in low magnetic field and at room temperature, prior to fast liquefaction of the polarized sample. The use of a new helium-3 cryostat enabled us to obtain liquid helium-3 with polarization rates up to 25 % at well-stabilized temperatures (around 0.5 K). We could thereby study the effect of nuclear polarization on liquid-vapour equilibrium, and particularly on the saturated vapour pressure. Very sensitive capacitive gauges were developed. We estimated (to first order in M{sup 2}) the expected effects when the polarization M is suddenly destroyed. These effects were experimentally observed in helium-3/helium-4 mixtures, in pure helium-3, only a transient increase in pressure has been recorded. We then describe in a third part a preliminary experiment which aimed at determining the longitudinal relaxation time T1 in mixtures. Relaxation on the walls is efficiently reduced by a cesium coating and T1s of order 20 minutes were observed. A careful determination of the helium-3 concentration in the liquid phase was made. Finally we studied the effects of dipolar field on transverse polarisation decay in our strongly polarized samples. We observed the free precession of polarization after a NMR pulse, and analysed in detail its decay time constant as a function of different parameters. This time constant drastically varied with the tipping angle, an effect which could be linked to NMR dynamical instabilities. (author)

  18. Experimental and numerical study of the active control of jets inside combustion chambers; Etude experimentale et numerique du controle actif de jets dans des chambres de combustion

    Energy Technology Data Exchange (ETDEWEB)

    Faivre, V.


    Combustion instabilities occur when the flame heat release couples with the acoustic waves propagating in the combustion chamber. This phenomenon can lead to strong vibrations and noise but also, sometimes, to the complete combustion device failure. That is the reason why so many studies focus on the control of those instabilities. The method chosen in this study consists in an active control device (or set of actuators) having a strong effect on the mixing of the burner exhaust flow with the ambient fluid. The model configuration studied consists in a non reactive jet of air controlled by four small tangential secondary jets. Experiments have been carried out to optimize the control device geometry. The configuration identified as the most efficient, in terms of mixing enhancement, has been simulated through Large Eddy Simulations (LES). The objective of the numerical part of the present work is double. First, the numerical simulations provide a better understanding of the phenomena occurring when the control is on. Then, it is shown that LES can be considered as a tool to predict the effects of a control device on a flow. (author)

  19. Air quality monitoring: experimental study of in-station automatic calibration; Surveillance de la qualite de l`air: etude experimentale portant sur le calibrage automatique en station

    Energy Technology Data Exchange (ETDEWEB)

    Clauss, G.; Paillarguest, T.; Walenda, R.; Pesch, P.; Gerboles, M.; Payrissat, M. [Association pour la Surveillance et l`Etude de la Pollution Atmospherique en Alsace, Schiltigheim (France)


    The analysis of the polluting gases that are regulated by the European directive (SO{sub 2}, NO/NO{sub 2}, O{sub 3}) is done by automatic analyzers which are regularly calibrated in monitoring stations. The automatic calibration by means of stationary devices, regardless of whether they are integrated into the analyser or not, is only applied in 60% of the stations in the European Union because of the uncertainty about the choice of the device and the costs. For over a year an in-situ study has been undertaken with the aim of testing six automatic calibration devices in the stations of the French network ASSPA and of the German Network UMEG. These devices were different in the type of calibration standard (bottle low concentration, dilution, permeation, titration in gas phase), in the diluting and measuring devices of the flow rate and in the procedure for acquiring the data. The test took into account the degree of automation of the control calibration as well as the automatic correction of the calibration differences and the constraints of the site. The verification of the stability of the tested equipment was guaranteed by a quality assurance programme of ERLAP. The test results have been evaluated in relation to the calibration quality in terms of stability and reliability of the devices and in relation to the investment costs and the running of the station. Of all the tested calibration devices, the diluter-type combined with a ozone generator, which also offers the possibility to run a titration cycle in gas phase, could be the choice likely to satisfy the needs of the stations. The other systems, less complete, give relatively acceptable results where the use of ozone generators for calibration purposes is excluded.

  20. Experimental study of turbulence on Tore Supra by plasma micro-waves interaction; Etude experimentale de la turbulence sur Tore Supra par interaction plasma micro-ondes

    Energy Technology Data Exchange (ETDEWEB)

    Colas, L


    Internal small-scale magnetic turbulence is a serious candidate to explain the anomalous heat transport in tokamaks. This turbulence is badly known in the gradient region of large machines. In this work internal magnetic fluctuations are measured on Tore Supra with an original diagnostic : Cross Polarization Scattering (CPS). This experimental tool relies on the Eigenmode change of a probing polarised microwave beam scattered by magnetic fluctuations, close to a cut-off layer for the incident wave. In this work, the diagnostic is first qualified to assess its sensitivity to magnetic fluctuations, and the spatial localisation for its measurements. The magnetic fluctuation behaviour is then analysed over a wide range of plasma current, density and additional power, and interpreted with a simple 1-D scattering model. A scan of the plasma density or magnetic field is used to move the CPS measurement location from r/a = 0.3 to r/a = 0.75. A fluctuation radial profile is obtained by two means. In L-mode discharges, the relation between magnetic fluctuations, temperature profiles and local heat diffusivities is investigated. With all measurements, it is also possible to look for a local parameter correlated to the turbulence in a large domain of plasma conditions. The fluctuation-induced local heat diffusivity expected from the measured fluctuations is estimated using the non-collisional quasi-linear formula: X{sup mag}{sub e} = {pi}qRV{sub te}({delta}B / B){sup 2}. Both the absolute values and the parametric dependence of calculated X{sup mag}{sub e} are close to the electron thermal diffusivities Xe determined by transport analysis. In particular, a threshold is evidenced in the dependence of fluctuation-induced heat fluxes on local {nabla}T{sub e}, which is analogous to the critical gradient for measured heat fluxes. The experimental setup is also sensitive to the Thomson scattering of the probing wave by density fluctuations. Its measurements are analysed as the fluctuations of the amplitude and the phase of an interferometer, thanks to a simple modelling of the plasma poloidal motion. Thus one than measure from the spectra shapes statistical quantities of the plasma movement. (author)

  1. Las Ciencias experimentales en el aula de Educación Infantil: El ciclo del agua


    Ribera Morán, María


    Este proyecto se basa en el ciclo de agua en relación a las ciencias experimentales, y enfocado al tercer curso del segundo ciclo de Educación Infantil, con alumnos de 5 a 6 años. En él se expondrán los diferentes autores que han dado lugar a este tipo de pedagogía activa, la metodología que se llevará a cabo, los objetivos que deberán alcanzar los alumnos, la programación y los procesos de evaluación que se pretenden, así como un apéndice con las actividades detalladas. Grado en Educación...

  2. Analyse experimentale des performances d'une batterie au lithium pour l'aeronautique (United States)

    Bonnin, Romain

    Ce memoire a pour objectif d'identifier et d'etudier les performances necessaires pour qu'une batterie au lithium puisse etre utilisee dans le secteur de l'aeronautique. C'est pourquoi dans le cadre de notre recherche, nous allons proposer une procedure de tests permettant d'analyser et de determiner si la batterie au lithium peut etre implantee dans un avion. En vue de repondre a l'analyse des performances, une etude des fonctionnalites demandees par l'avion ainsi que des normes preexistantes vont etre effectuees. Suite a cette etape, nous allons elaborer un banc d'essais. Une fois le banc d'essais acheve, nous allons tester une batterie au lithium qui est supposee disposer de toutes les caracteristiques techniques requises pour etre implantee dans un avion. Ces tests nous permettront donc d'emettre un avis sur l'utilisation des batteries au lithium dans le domaine de l'aeronautique.

  3. Aerodynamic study of the fluid flow in the channel of a reactor filled with internally and externally cooled fuel elements; Etude aerodynamique de l'ecoulement fluide dans un canal de reacteur charge en elements combustibles annulaires refroidis interieurement et exterieurement

    Energy Technology Data Exchange (ETDEWEB)

    Rousseau, J.C. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    A study is made of the problem of the flow-rate and pressure distributions along the length of two volumes, internal and external, bounded by a series of non-continuous annular elements placed along the channel axis. It is observed that the phenomenon can easily be represented by equations. The theoretical expressions observed are particularly simple when the distances between the elements are above a certain minimum value. The experimental work has made it possible to show that the theoretical formulation derived is valid with a very great accuracy. The experimental study has also been carried out in the case of a very small spacing between the elements. It has been possible to show in this case that the hypothesis made for deriving the theoretical expressions was perfectly justified. In the last part finally, we consider the practical problem of evaluating the pressure-drops between the ends of a series of annular elements. (author) [French] On etudie le probleme de la repartition des debits et des pressions le long des deux espaces, interne et externe, delimites par une succession d'elements annulaires non jointifs, disposes suivant l'axe d'un canal. On constate que le phenomene peut etre mis aisement en equation. Les relations theoriques obtenues prennent en particulier une forme simple lorsque les intervalles entre elements sont superieurs a une valeur minimum. L'etude experimentale a permis de constater que cette formulation theorique etablie etait alors valable avec une precision excellente. L'etude experimentale a ete egalement effectuee dans le cas de tres faibles intervalles entre elements consecutifs. On a pu alors verifier que l'hypothese adoptee dans l'etablissement des relations theoriques etait parfaitement justifiee. Dans un dernier chapitre enfin, nous abordons le point de vue pratique de l'evaluation des pertes de charge aux bornes d'un train d'elements annulaires. (auteur)

  4. Bacterial Keratitis (United States)

    ... Español Eye Health / Eye Health A-Z Bacterial Keratitis Sections What Is Bacterial Keratitis? Bacterial Keratitis Symptoms ... Lens Care Bacterial Keratitis Treatment What Is Bacterial Keratitis? Leer en Español: ¿Qué Es la Queratitis Bacteriana? ...

  5. PENULISAN ETUDE-ETUDE MUSIK TALEMPONG UNGGAN (Sebuah Usaha Pembelajaran Musik Tradisi Berbasis Literatur

    Directory of Open Access Journals (Sweden)

    Asri MK


    Full Text Available “Talempong unggan”, a traditional music from Minangkabau community particularly in Unggan, Sumpur Kudus, Sijunjung Regency, West Sumatera Indonesia is classified into genre of “talempong duduak” (rea. Due to its special musical concept and the playing technics, this traditional music is selected as a practising course in the Karawitan Department of Indonesian Institute of Art (ISI Padang Panjang since 1993 till now. In a system of class learning with many students, Talempong Unggan definitely needs supporting methods and learning technics suitable for the course where the students can reach their maximum skills. All the melody of “talempong unggan” that has been made as a material of practice is transcribed to the system of numeric notation and rhythm motive of “gendang” and “aguang” which is written into signs and special notation. All of qualitative data is formulated into finding methods, technics and etude of learning ensamble of Talempong Unggan the traditional music that learned in Karawitan Department of ISI Padang Panjang. Key words: Talempong Unggan, Methods, Technics, Etude

  6. Etude des interactions entre les differents acteurs des interventions ...

    African Journals Online (AJOL)

    Etude des interactions entre les differents acteurs des interventions sous directives communautaires et changements obtenus au Benin et au Togo. P.V. Malou Adom, Ch. P. Makoutode, T Gnaro, A.R. Ouro-Koura, E.M. Ouendo, G Napo-Koura, M Makoutode ...

  7. Implementing Formative Assessment in Engineering Education: The Use of the Online Assessment System Etude (United States)

    Dopper, Sofia M.; Sjoer, Ellen


    This article describes the possibilities offered by the online assessment system Etude to achieve the benefits of formative assessment. In order to find out the way this works in practice, we carried out an experiment with the use of Etude for formative assessment in the course on collaborative report writing. Results show that online formative…

  8. Bacterial meningitis

    NARCIS (Netherlands)

    Roos, Karen L.; van de Beek, Diederik


    Bacterial meningitis is a neurological emergency. Empiric antimicrobial and adjunctive therapy should be initiated as soon as a single set of blood cultures has been obtained. Clinical signs suggestive of bacterial meningitis include fever, headache, meningismus, vomiting, photophobia, and an

  9. 98 Etude Comparative du Systeme Phonematique et Tonal des ...

    African Journals Online (AJOL)

    98. Etude Comparative du Systeme Phonematique et Tonal des. Deux Parlers: Le Yoruba du Nigeria et L'itsa Parle en. Republique du Benin. Ajani Akinwumi Lateef et Ayuba G. Ajibabi http://dx.doi./org/10.4314/ujah.v18i3.5. Résume. En ce début de l'époque ou la mondialisation et la coexistence des peuples dans le ...

  10. Un estudio del contenido científico de las preguntas formuladas por estudiantes cuando intentan comprender dispositivos experimentales


    Torres Valois, Tarcilo; Milicic, Beatriz; Sanjosé López, Vicente


    Se muestra un análisis descriptivo del contenido científico de las preguntas formuladas por estudiantes de secundaria y universitarios, cuando tratan de comprender el funcionamiento de dispositivos experimentales. Se consideran tres situaciones didácticas habituales: leer un texto acompañado de imágenes estáticas (esquemáticas); visualizar imágenes dinámicas (video o demostración de cátedra), observar y manipular los dispositivos. Solo una tercera parte de las preguntas de los estudiantes con...

  11. Inteligencias múltiples aplicadas en las carreras de pedagogía de las ciencias experimentales


    Zapata Villacis, Luis Alberto


         Este estudio presenta el desarrollo alcanzado por los estudiantes que cursan el primero y segundo semestre de las carreras de Pedagogía de las Ciencias Experimentales: Informática y Matemática de la Facultad de Filosofía, Letras y Ciencias de la Educación, de la Universidad Central del Ecuador, en las diferentes inteligencias aprendidas durante las  etapas del proceso educativo, aplicando el modelo de Gardner sobre las inteligencias múltiples, con 75 ítems; en el...

  12. Análisis de Sistemas Experimentales de Integración Peces-Pollos.

    Directory of Open Access Journals (Sweden)

    Roberto Trejo Albarrán


    Full Text Available In this work the objective was to evaluate four treatments using dung of different kinds from experimental cultures, estimating their efficiency in relation to pisciculture production and the characteristics of each pond. The work was carried out in the Laboratorio de Hidrobiología of the Universidad Autónoma del Estado de Morelos (UAEM in Cuernavaca, Morelos, Mexico, in fiberglass ponds with albinotic Oreochromis niloticus. Analysis was made of plankton, as well as bacteriological, dietectic and piscicultural growth and the physical chemistry of water. Fresh chicken excrement showed variations in the amount of nutriments. Abundance of phytoplankton was from 17,008 to 70,080 org/ml. The most abundant was Closterium sp. In zooplankton, total abundance was from 302 to 1,706 org/ml. The dominant zooplankton was Moina sp. with a yield from 22 to 117 g/m2/ 6 months. Considering these findings, it was observed that the digested chicken dung was the most efficient of the four. Pathogenic enterobacteria were always present. En este trabajo, el objetivo fue evaluar cuatro tratamientos utilizando estiércoles de diferentes tipos en cultivos experimentales, estimando su eficiencia en relación a la producción piscícola y las características de cada estanque. Se llevó a cabo en el Laboratorio de Hidrobiología de la UAEM en Cuernavaca, Morelos, México; en estanques de fibra de vidrio, con Oreochromis niloticus albina. Se realizaron análisis del plancton, bacteriológicos, bromatológicos, crecimiento piscícola y fisicoquímicos del agua. Las excretas frescas de pollo presentaron variaciones en la cantidad de nutrimentos. Las abundancias de fitoplancton fueron de 17,008 a 70,080 org/ml. Los más abundantes fueron Closterium sp. En el zooplancton, las abundancias totales fueron de 302 a 1,706 org/ml. El dominante fue Moina sp. El rendimiento fue de 22 a 117 g/m2/6 meses. Considerando lo anterior se observó que la gallinaza digerida fue el esti

  13. Enfermedad de Huntington: modelos experimentales y perspectivas terapéuticas

    Directory of Open Access Journals (Sweden)

    Teresa Serrano Sánchez


    Full Text Available La enfermedad de Huntington (EH es un trastorno degenerativo de origen hereditario. Hasta el momento no existe un tratamiento efectivo para la enfermedad que inexorablemente después de transcurridos 15 a 20 años, evoluciona hacia la incapacidad total o la muerte. En este trabajo se revisan las características clínicas y morfológicas de la EH y los modelos experimentales más utilizados para su estudio tomando como fuente, los artículos indexados en la base de datos Medline publicados en los últimos 20 años. Se valoran las ventajas y desventajas de estos modelos y su perspectiva para el desarrollo de ensayos clínicos. El consenso de lo reportado plantea que de los modelos tóxicos, los inducidos por neurotoxinas tales como el ácido quinolínico parecen ser los más adecuados para reproducir las características neuropatológicas, y por otro lado los modelos genéticos contribuyen con más evidencias al conocimiento del origen etiológico de la enfermedad. Numerosos tratamientos han sido aplicados en el manejo de las manifestaciones clínicas que aparecen en la EH, sin poder detener o disminuir las afectaciones que derivan de la pérdida neuronal. La sintomatología clínica ha sido posible reproducirla, al menos en parte, en animales de experimentación lo que ha permitido realizar ensayos terapéuticos. Desde el punto de vista de tratamiento, lo que más promisorio parece ser, es el trasplante de fuentes celulares y dentro de ellas las no neurales, que implican menor censura ética y mayor factibilidad de obtención para la aplicación en los enfermos. Por otro lado el desarrollo de la tecnología del ARN interferente, emerge como una herramienta terapéutica potencial para el tratamiento de la EH, así como para responder interrogantes básicas relacionadas con el desarrollo de la enfermedad.

  14. Mise au point du rheometre a cisaillement annulaire pour l'etude de rheologie d'un mastic bitumineux avec verre incorpore = (United States)

    El Mansouri, Souleimane

    Dans le domaine viscoelastique lineaire (VEL, domaine des petites deformations), le comportement thermomecanique du bitume et du mastic bitumineux (melange uniforme de bitume et de fillers) a ete caracterise au Laboratoire des Chaussees et Materiaux Bitumineux (LCMB) de l'Ecole de technologie superieure (ETS) avec l'appui de nos partenaires externes : la Societe des Alcools du Quebec (SAQ) et Eco Entreprises Quebec (EEQ). Les proprietes rheologiques des bitumes et des mastics ont ete mesurees grâce a un nouvel outil d'investigation appele, Rheometre a Cisaillement Annulaire (RCA), sous differentes conditions de chargement. Cet appareil permet non seulement de solliciter des eprouvettes de tailles importantes par rapport a celles utilisees lors des essais classiques, mais aussi d'effectuer des essais en conditions quasi-homogenes, ce qui permet de donner acces a la loi de comportement des materiaux. Les essais sont realises sur une large gamme de temperatures et de frequences (de -15 °C a 45°C et de 0,03Hz a 10 Hz). Cette etude a ete menee principalement pour comparer le comportement d'un bitume avec celui d'un mastic bitumineux dans le domaine des petites deformations. neanmoins, dans une seconde perspective, on s'interesse a l'influence des fillers de verre de post-consommation sur le comportement d'un mastic a faibles niveaux de deformations en comparant l'evolution des modules complexes de cisaillements (G*) d'un mastic avec fillers de verre et un mastic avec fillers conventionnels (calcaire). Enfin, le modele analogique 2S2P1D est utilise pour simuler le comportement viscoelastique lineaire des bitumes et des mastics bitumineux testes lors de la campagne experimentale.

  15. Bacterial Proteasomes. (United States)

    Jastrab, Jordan B; Darwin, K Heran


    Interest in bacterial proteasomes was sparked by the discovery that proteasomal degradation is required for the pathogenesis of Mycobacterium tuberculosis, one of the world's deadliest pathogens. Although bacterial proteasomes are structurally similar to their eukaryotic and archaeal homologs, there are key differences in their mechanisms of assembly, activation, and substrate targeting for degradation. In this article, we compare and contrast bacterial proteasomes with their archaeal and eukaryotic counterparts, and we discuss recent advances in our understanding of how bacterial proteasomes function to influence microbial physiology.

  16. Bacterial adhesion

    NARCIS (Netherlands)

    Loosdrecht, van M.C.M.


    As mentioned in the introduction of this thesis bacterial adhesion has been studied from a variety of (mostly practice oriented) starting points. This has resulted in a range of widely divergent approaches. In order to elucidate general principles in bacterial adhesion phenomena, we felt it

  17. L'etude de l'InP et du GaP suite a l'implantation ionique de Mn et a un recuit thermique (United States)

    Bucsa, Ioan Gigel

    Cette these est dediee a l'etude des materiaux InMnP et GaMnP fabriques par implantation ionique et recuit thermique. Plus precisement nous avons investigue la possibilite de former par implantation ionique des materiaux homogenes (alliages) de InMnP et GaMnP contenant de 1 a 5 % atomiques de Mn qui seraient en etat ferromagnetique, pour des possibles applications dans la spintronique. Dans un premier chapitre introductif nous donnons les motivations de cette recherche et faisons une revue de la litterature sur ce sujet. Le deuxieme chapitre decrit les principes de l'implantation ionique, qui est la technique utilisee pour la fabrication des echantillons. Les effets de l'energie, fluence et direction du faisceau ionique sur le profil d'implantation et la formation des dommages seront mis en evidence. Aussi dans ce chapitre nous allons trouver des informations sur les substrats utilises pour l'implantation. Les techniques experimentales utilisees pour la caracterisation structurale, chimique et magnetique des echantillons, ainsi que leurs limitations sont presentees dans le troisieme chapitre. Quelques principes theoriques du magnetisme necessaires pour la comprehension des mesures magnetiques se retrouvent dans le chapitre 4. Le cinquieme chapitre est dedie a l'etude de la morphologie et des proprietes magnetiques des substrats utilises pour implantation et le sixieme chapitre, a l'etude des echantillons implantes au Mn sans avoir subi un recuit thermique. Notamment nous allons voir dans ce chapitre que l'implantation de Mn a plus que 1016 ions/cm 2 amorphise la partie implantee du materiau et le Mn implante se dispose en profondeur sur un profil gaussien. De point de vue magnetique les atomes implantes se trouvent dans un etat paramagnetique entre 5 et 300 K ayant le spin 5/2. Dans le chapitre 7 nous presentons les proprietes des echantillons recuits a basses temperatures. Nous allons voir que dans ces echantillons la couche implantee est polycristalline et les

  18. Bacterial Vaginosis (United States)

    ... Archive STDs Home Page Bacterial Vaginosis (BV) Chlamydia Gonorrhea Genital Herpes Hepatitis HIV/AIDS & STDs Human Papillomavirus ( ... of getting other STDs, such as chlamydia and gonorrhea . These bacteria can sometimes cause pelvic inflammatory disease ( ...

  19. Diseño de prácticas experimentales de fotosíntesis para ciclo 3


    Macías Yomayusa, Luis Felipe


    La fotosíntesis es un proceso fundamental para la vida en el planeta, además es uno de los temas de gran importancia educativa para la enseñanza de la biología. Sin embargo, también es uno de los capítulos más difíciles de enseñar y de aprender, por lo abstracto y extenso. De allí que sea necesario implementar estrategias que promuevan su aprendizaje y comprensión. En este trabajo se presenta el diseño de dos guías de prácticas experimentales de fotosíntesis para ciclo 3 de básica secundaria,...


    Directory of Open Access Journals (Sweden)

    Payel Sarkar


    Full Text Available Petroleum aromatic hydrocarbons like benzen e, toluene, ethyl benzene and xylene, together known as BTEX, has almost the same chemical structure. These aromatic hydrocarbons are released as pollutants in th e environment. This work was taken up to develop a solvent tolerant bacterial cons ortium that could degrade BTEX compounds as they all share a common chemical structure. We have isolated almost 60 different types of bacterial strains from different petroleum contaminated sites. Of these 60 bacterial strains almost 20 microorganisms were screene d on the basis of capability to tolerate high concentration of BTEX. Ten differe nt consortia were prepared and the compatibility of the bacterial strains within the consortia was checked by gram staining and BTEX tolerance level. Four successful mi crobial consortia were selected in which all the bacterial strains concomitantly grew in presence of high concentration of BTEX (10% of toluene, 10% of benzene 5% ethyl benzene and 1% xylene. Consortium #2 showed the highest growth rate in pr esence of BTEX. Degradation of BTEX by consortium #2 was monitored for 5 days by gradual decrease in the volume of the solvents. The maximum reduction observed wa s 85% in 5 days. Gas chromatography results also reveal that could completely degrade benzene and ethyl benzene within 48 hours. Almost 90% degradation of toluene and xylene in 48 hours was exhibited by consortium #2. It could also tolerate and degrade many industrial solvents such as chloroform, DMSO, acetonitrile having a wide range of log P values (0.03–3.1. Degradation of aromatic hydrocarbon like BTEX by a solvent tolerant bacterial consortium is greatly significant as it could degrade high concentration of pollutants compared to a bacterium and also reduces the time span of degradation.

  1. Bacterial Ecology

    DEFF Research Database (Denmark)

    Fenchel, Tom


    Bacterial ecology is concerned with the interactions between bacteria and their biological and nonbiological environments and with the role of bacteria in biogeochemical element cycling. Many fundamental properties of bacteria are consequences of their small size. Thus, they can efficiently exploit...

  2. Bacterial meningitis

    NARCIS (Netherlands)

    Heckenberg, Sebastiaan G. B.; Brouwer, Matthijs C.; van de Beek, Diederik


    Bacterial meningitis is a neurologic emergency. Vaccination against common pathogens has decreased the burden of disease. Early diagnosis and rapid initiation of empiric antimicrobial and adjunctive therapy are vital. Therapy should be initiated as soon as blood cultures have been obtained,

  3. Bacterial lipases

    NARCIS (Netherlands)

    Jaeger, Karl-Erich; Ransac, Stéphane; Dijkstra, Bauke W.; Colson, Charles; Heuvel, Margreet van; Misset, Onno

    Many different bacterial species produce lipases which hydrolyze esters of glycerol with preferably long-chain fatty acids. They act at the interface generated by a hydrophobic lipid substrate in a hydrophilic aqueous medium. A characteristic property of lipases is called interfacial activation,

  4. Bacterial Ecology

    DEFF Research Database (Denmark)

    Fenchel, Tom


    , the production and oxidation of methane, nitrate reduction and fixation of atmospheric nitrogen are exclusively carried out by different groups of bacteria. Some bacterial species – ‘extremophiles’ – thrive in extreme environments in which no eukaryotic organisms can survive with respect to temperature, salinity...

  5. Bacterial Vaginosis (United States)

    ... that coats the walls of the vagina Vaginal discharge with an unpleasant or fishlike odor Vaginal pain or itching Burning during urination Doctors are unsure of the incubation period for bacterial vaginosis. How Is the Diagnosis Made? Your child’s pediatrician can make the diagnosis ...

  6. Bacterial stress

    Indian Academy of Sciences (India)

    First page Back Continue Last page Graphics. Bacterial stress. Physicochemical and chemical parameters: temperature, pressure, pH, salt concentration, oxygen, irradiation. Nutritional depravation: nutrient starvation, water shortage. Toxic compounds: Antibiotics, heavy metals, toxins, mutagens. Interactions with other cells: ...

  7. Treatment of effluent at the Saclay Centre d'Etudes Nucleaires (1960); Le traitement des effluents du Centre d'Etudes Nucleaires de Saclay (1960)

    Energy Technology Data Exchange (ETDEWEB)

    Wormser, G. [Commissariat a l' Energie Atomique, Saclay (France).Centre d' Etudes Nucleaires


    The Centre d'Etudes Nucleaires at Saclay possesses several installations from which liquid radioactive effluent is rejected, and it has thus been found necessary to construct a station for the purification of radioactive liquids and to settle various chemical, analytical and technological problems. This report describes, in the following order: - the disposal possibilities at the Centre d'Etudes Nucleaires, Saclay, - the effluents produced at the centre, - the set-up for collecting effluent, - treatment of the effluent, - results of these treatments. (author) [French] La presence, au Centre d'Etudes Nucleaires de Saclay, de plusieurs installations susceptibles de rejeter des effluents liquides radioactifs a necessite la construction d'une station d'epuration d'eaux radioactives et la mise au point de differents problemes chimiques, analytiques et technologiques. Dans ce rapport, nous exposerons successivement: - les possibilites de rejet du Centre d'Etudes Nucleaires de Saclay, - les effluents du centre, - le dispositif de collecte des effluents, - le traitement de ces effluents, - les resultats de ces traitements. (auteur)

  8. Bacterial Adhesion & Blocking Bacterial Adhesion

    DEFF Research Database (Denmark)

    Vejborg, Rebecca Munk


    reduce or delay bacterial biofilm formation of a range of urinary tract infectious E.coli and Klebsiella isolates. Several other proteinaceous coatings were also found to display anti-adhesive properties, possibly providing a measure for controlling the colonization of implant materials. Several other...... components. These substances may both mediate and stabilize the bacterial biofilm. Finally, several adhesive structures were examined, and a novel physiological biofilm phenotype in E.coli biofilms was characterized, namely cell chain formation. The autotransporter protein, antigen 43, was implicated...

  9. Bacterial lipases


    Jaeger, Karl-Erich; Ransac, Stéphane; Dijkstra, Bauke W.; Colson, Charles; Heuvel, Margreet van; Misset, Onno


    Many different bacterial species produce lipases which hydrolyze esters of glycerol with preferably long-chain fatty acids. They act at the interface generated by a hydrophobic lipid substrate in a hydrophilic aqueous medium. A characteristic property of lipases is called interfacial activation, meaning a sharp increase in lipase activity observed when the substrate starts to form an emulsion, thereby presenting to the enzyme an interfacial area. As a consequence, the kinetics of a lipase rea...

  10. Diseño de una aleación termorresistente base hierro para hornos Herreshoff. Parte II- Resultados experimentales

    Directory of Open Access Journals (Sweden)

    Antonio R. Chang-Cardona


    Full Text Available A partir del diseño de una nueva aleación Fe-Cr-Ni-C-Al con las propiedades deseadas para fabricar brazos de hornos de reducción Herreshoff,  se obtuvieron las muestras y se investigó la influencia del tratamiento térmico en la microestructura y la formación de fases utilizano la microscopía óptica y la microscopía electrónica de barrido, conjuntamente con la difracción de rayos X. Se evaluaron las propiedades mecánicas a temperatura ambiente y a altas temperaturas a través de los ensayos de tracción y termofluencia. Los resultados experimentales obtenidos se corresponden con los pronósticos del diseño en lo que respecta a la formación de fases, las tensiones fluencia y las tensiones de rotura demostrando así la posibilidad  de obtener una aleación con una durabilidad de 100 000 h a 800 °C bajo tensiones de 180 MPa. Por lo tanto, el procedimiento de diseño empleado es validado y puede considerarse una propuesta novedosa para el diseño y desarrollo de nuevas aleaciones.

  11. Modifications enzymatiques sanguines chez la canette mularde (Catrina moschata x Anas platyrhynchos) lors d'infection experimentale par Eimeria mulardi. (United States)

    Pascalon-Pekelniczky, A; Michoudet, C; Chauve, C M


    Les modifications sanguines de phosphatase alcaline (PA), acide aspartique amino-transferase (AsAT), alanine aminotransferase (A1AT), creatine phosphokinase (CPK) et lactate deshydrogenase (LDH) ont ete recherchees chez 48 canards mulards femelles infectes experimentalement a l'age de 11 jours (JO) avec 5 x 10(3), 5 X 10(4) ou 5 X 10(5) oocystes sporules d'Eimeria mulardi: les controles ont ete realises de J - 1 a J25 apres infection et les valeurs comparees a celles d'un lot temoin. La mortalite et la diminution de croissance n'ont ete observees qu'avec 5 x 10(5) oocystes, a partir de J6. La PA est significativement diminuee avec 5 x 10(4 )et 5 x 10(5) oocystes, de facon precoce (J5 et J3 respectivement). Les taux minimaux sont observes a J9: respectivement 83 et 35% des valeurs temoin. Cette chute de la PA pourrait etre utilisee dans un but de diagnostic. Les variations d'AsAT, A1AT, CPK et LDH semblent moins specifiques de l'infection par E. mulardi.

  12. Study of a case of essential hypoproteinaemia using the isotopes {sup 131}I and {sup 15}N; Etude a l'aide des isotopes {sup 131}I et {sup 15}N d'un cas d'hypoproteinemie essentielle

    Energy Technology Data Exchange (ETDEWEB)

    Dubert, A.; Coursaget, J.; Fallot, P.; Royer, P. [Commissariat a l' Energie Atomique, Saclay (France).Centre d' Etudes Nucleaires; Hopital des Enfants Malades, 75 - Paris (France)


    Hypoproteinaemia both human and experimental has been studied with the help of nuclear labelling. A comparative study has been made of the fate of serum proteins labelled by an endogenous path after administration of nitrogen-15 glycine and also of serum albumin labelled by exogenous path using iodine-131. We have determined the biological period of the serum albumin and serum globulins and also the magnitude of the vascular and extravascular pools of serum albumin. In this way it has been possible to measure the speed of replacement of plasma proteins. A presentation of results taken from both clinical observation and experiment leads to a discussion on the pathogenic mechanism of hypoproteinaemia. (author) [French] Les hypoproteinemies humaine et experimentale ont ete explorees a l'aide d'indicateurs nucleaires. Une etude comparative du devenir des proteines seriques marquees par voie endogene, apres administration de glycocolle-{sup 15}N et de l'albumine serique marquee par voie exogene a l'iode-131 a ete realisee. On a determine la periode biologique de la serumalbumine et des globulines seriques ainsi que la grandeur des 'pools' vasculaire et extravasculaire de la serumalbumine. Il a ete ainsi possible d'evaluer la vitesse de renouvellement des proteines plasmatiques. La confrontation des resultats tires de l'observation clinique et de l'experimentation conduit a une discussion sur le mecanisme pathogenique de l'hypoproteinemie. (auteur)

  13. Treatment of effluent at the Saclay Centre d'Etudes Nucleaires (1960)

    International Nuclear Information System (INIS)

    Wormser, G.


    The Centre d'Etudes Nucleaires at Saclay possesses several installations from which liquid radioactive effluent is rejected, and it has thus been found necessary to construct a station for the purification of radioactive liquids and to settle various chemical, analytical and technological problems. This report describes, in the following order: - the disposal possibilities at the Centre d'Etudes Nucleaires, Saclay, - the effluents produced at the centre, - the set-up for collecting effluent, - treatment of the effluent, - results of these treatments. (author) [fr

  14. Study of secondary electronic emission in some piezo-electric materials: application to ultrasonic visualization; Etude de l'emission electronique secondaire de quelques materiaux piezoelectriques: application a la visualisation ultrasonore

    Energy Technology Data Exchange (ETDEWEB)

    Marini, J. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    Methods allowing the visualization of acoustic images appear at the moment to be of great interest in the field of non-destructive testing as well as in that of underwater detection. In order to carry out certain calculations on the operation of an ultrasonic camera, it has been necessary to study the secondary electron emission of some piezoelectric materials liable to be incorporated into the equipment. The secondary electron emission of insulators is a rather complex phenomenon; in order to find a rational explanation for the observations made, a theory has been developed for the energy spectrum of the emitted electrons. The experimental results of this work have then been used to build an ultrasonic visualization installation. Some examples of acoustic images which have been visualized are also presented. (author) [French] Les methodes qui permettent de visualiser des images acoustiques trouvent a l'heure actuelle un grand interet dans le domaine du controle non destructif comme dans celui de la detection sous-marine. De maniere a effectuer certains calculs sur le fonctionnement d'une camera ultrasons, il a ete necessaire d'etudier l'emission electronique secondaire de quelques materiaux piezoelectriques susceptibles d'etre utilises dans sa construction. L'emission electronique secondaire des isolants est un phenomene assez complexe et de maniere a trouver des explications coherentes aux observations effectuees, une theorie du spectre energetique des electrons emis a ete elaboree. Une installation de visualisation ultrasonore a alors ete realisee a partir des donnees experimentales de cette etude. Quelques exemples d'images acoustiques visualisees par cette methode sont egalement presentees. (auteur)

  15. Contribution to the study of detonation initiation by shock in solid explosives (1961); Contribution a l'etude de l'initiation de la detonation par choc dans les explosifs solides (1961)

    Energy Technology Data Exchange (ETDEWEB)

    Fauquignon, C. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    When a shock wave is induced in an explosive, it can initiate chemical reactions which lead more or less rapidly to a stable detonation state. In this study more particular attention was paid to the transition phase, in which has been evaluated the increases in the wave velocity, the pressure, and the electrical conductivity. The influence of the nature of the medium in front of the wave and in contact with the explosive has also been the subject of an experimental study designed to determine the extent to which its nature can be ignored and, subsequently, to characterise the initiation conditions using only the shock intensity induced in the explosive. Finally, the results were generalized for various explosive compositions and led to the development of a possible mechanism for shock initiation. (author) [French] Lorsqu'une onde de choc est induite dans un explosif, elle peut y amorcer des reactions chimiques qui conduisent plus ou moins rapidement a un regime de detonation stable. Dans cette etude, on s'est interesse plus particulierement a la phase transitoire dans laquelle on a evalue l'accroissement de la vitesse d'onde, de la pression et de la conductibilite electrique. L'influence du milieu amont, au contact de l'explosif, a egalement fait l'objet de recherches experimentales de facon a determiner dans quelle mesure on pouvait s'affranchir de sa nature et, par suite, caracteriser les conditions d'initiation par la seule intensite du choc induit dans l'explosif. Enfin, les resultats ont ete generalises a diverses compositions explosives et ont conduit a l'elaboration d'un schema possible du mecanisme de l'initiation par choc. (auteur)

  16. Proton scattering from unstable nuclei {sup 20}O, {sup 30}S, {sup 34}Ar: experimental study and models; Diffusion de protons sur les noyaux instables {sup 20}O, {sup 30}S, {sup 34}Ar: etude experimentale et developpement de modeles

    Energy Technology Data Exchange (ETDEWEB)

    Khan, Elias [Institut de Physique Nucleaire, CNRS - IN2P3, Universite Paris - Sud, 91406 Orsay Cedex (France)


    Elastic and inelastic proton scattering from the unstable nuclei {sup 20}O, {sup 30}S and {sup 34}Ar were measured in inverse kinematics at the Grand Accelerateur National d'Ions Lourds. Secondary beams of {sup 20}O at 43 MeV/A, {sup 30}S at 53 MeV/A and {sup 34}Ar at 47 MeV/A impinged on a (CH{sub 2}){sub n} target. Recoiling protons were detected in the silicon strip array MUST. Energies and angular distributions of the first 2{sup +} and 3{sup -} states were measured. A phenomenological analysis yields values of the deformation parameters {beta}{sub 2} and {beta}{sub 3} of 0.55 (6) and 0.35 (5) for {sup 20}O, 0.32 (3) and 0.22 (4) for {sup 30}S, 0.27 (2) and 0.39 (3) for {sup 34}Ar, respectively, and allows the extraction of the ratio of neutron to proton transition matrix elements (M{sub n}/M{sub p})/(N/Z) for 2{sup +} states: 2.35 (37) for {sup 20}O, 0.93 (20) for {sup 30}S and 1.35 (28) for {sup 34}Ar. Therefore the proton rich nuclei {sup 30}S and {sup 34}Ar show a 2{sup +} excitation of isoscalar character whereas the excitation of {sup 20}O is of isovector character. In order to perform a microscopic analysis of the data, we have developed a QRPA model, using three Skyrme interaction: SIII, SG2, SLy4. This model reproduces measured B(EL) values for the oxygen, sulfur and argon isotopic chains, whereas RPA calculations, which do not take pairing into account, underestimate these values. In the case of the QRPA model the energies of the first 2{sup +} state are overestimated by about 1 MeV, but the evolution along the isotopic chains is well reproduced. (M{sub n}/M{sub p})/(N/Z) ratios for the first 2{sup +} state deduced from the microscopic analysis using QRPA are 1.98 for {sup 20}O, 1.05 for {sup 30}S and 1.00 for {sup 34}Ar, in agreement with the conclusions of the phenomenological analysis. However important discrepancies are observed between the two types of analysis for other isotopes, in particular neutron rich argon and sulfur nuclei. (author)

  17. Chemical and radiological toxicity of iodine isotopes. Experimental study on the rat at the perinatal stage; Toxicite chimique et radiologique des isotopes de l'iode. Etude experimentale chez le rat en periode perinatale

    Energy Technology Data Exchange (ETDEWEB)

    Bourahla, K


    The recommended prophylactic measure in the case of an exposure to radio-iodine is an excess take of stable iodine. During the perinatal stage, the thyroid is radio-sensible but also fragile with respect to an excess of iodine. This work performed on the rat, treats of the potential thyroidal toxicity of the prophylaxy and analyzes the early radio-lesions induced by {sup 131}I. On the basis of microscopic (optical, electronic, ionic) and dosimetric studies (TSH, T4), four aspects are considered: 1 - the perinatal morpho-functional evolution (F18, J1, J4, J10, J21, J35); 2 - the consequences of an iodine overburden at three moments of the thyroid maturation (F16, F20 and J4); 3 - the effects on the thyroid cells growth of different iodine overburdens (4 g, 20 g, 100 g); and 4 - the radio-toxic effects (after 48 h) of {sup 131}I taken at J5 (30 Gy) and at J35 (900 Gy). This work evidences the following points: 1 - the perinatal evolution of the thyroid tissue of the rat shows ultra-structural and follicular modifications and physiological follicular destructions; 2 - the variability of the iodine overburden effects: hyperactivity for overburdens at F16, tissue destruction with compensated hypothyroidism for overburdens at J5, no recognized thyroidal anomaly for the overburden at J20; 3 - the iodated overburden inhibits the start-up of the S-phase of the cellular cycle at a lower level (1/20); 4 - the {sup 131}I taken at J5 (30 Gy) induces a lysis of the nucleic acids content, while {sup 131}I taken at J35 (900 Gy) induces an important inflammatory reaction and some apoptosis phenomena. In summary, the stable iodine prophylactic measure can have two conjugated effects on the rat: an interesting action of thyroid cells growth inhibition, and a toxic action leading to an hyperactivity or a follicular destruction without hypothyroidism, depending on the maturation stage. The early effects of {sup 131}I seem to be linked with the age. (J.S.)

  18. Modelling and experimental study of the behavior of radon and radon decay products in an enclosure. Application to houses; Modelisation et etude experimentale du comportement du radon et de ses descendants dans une enceinte confinee. Application a une habitation

    Energy Technology Data Exchange (ETDEWEB)

    Gouronnec, A.M.


    Since the eighties, more and more studies were performed about radon and its decay products in houses with one of the aim being the estimation of the dose received by their inhabitants. Then, the principal objective of this work is to describe the behaviour of radon and its decay products within a dwelling. In the first part to the report, a few definitions are given and data from literature give an idea of indoor radon and radon decay products activities and/or size distribution. Aspects of dosimetry are presented too. In the second part of the work, a mathematical model, called `PRADDO` of Physic of Radon and radon Decay products in Domestic environment is developed on the basis of the classical model written by Jacobi in 1972. On the one hand, it has to predict radon decay products activities in systems consisting in one or more enclosure(s), from radon activity and from ambient aerosol concentration and size distribution. On the other hand, one part of the model is assigned to study the influence of the entry model parameters variation on the calculated quantities. Then, in the third part of the work, two experimental studies are realised in order to compare measurements to modelization. The first experimentation is a laboratory work, made on the test bench ICARE from IPSN, and the second one consists in describing the basement of an occupied house from Brittany. In the two cases, the comparison between experiments and modelling shows a good agreement if particles are present in the air, but any conclusion is made when is no aerosol in the enclosure. (author). 158 refs., 81 figs., 42 tabs.

  19. Experimental study of the vaporization of a droplets injection in a fluidized gas-solid media; Etude experimentale de la vaporisation d'un jet de goutelettes au contact d'un milieu gaz-solide fluidise

    Energy Technology Data Exchange (ETDEWEB)

    Leclere, K.


    The quality of feedstock injection in the Fluid Catalytic Process (FCC) is essential to ensure a good vaporization. The vaporization should be fast so that the cracking reaction in the gaseous phase can happen within the short residence time in the riser (a few seconds). Vaporization is helped by a uniform injection of droplets as small as possible as well as a good mixing with the catalyst particles that represent the main heat source. Several models were developed to predict the droplet vaporization in a gas-solid media. However, no experimental validation exists for these models, whose predictions vary (from 1 to several hundreds of milliseconds). The objective of this study was to get a better understanding of the physical phenomena taking place during droplet vaporization. This was done in two steps. First, operating limits had to be defined to ensure an optimal vaporization and to avoid local saturation and agglomerate formation. These limits were precisely determined under laboratory conditions to validate a model that showed that agglomeration does not occur under industrial conditions. Then, a kinematic study of vaporization under operating conditions without agglomerate formation was performed. An original measurement technique was developed to get samples at very short times (tens of milliseconds). Experiments showed that heat transfer was not limiting and that mass transfer was the limiting process during vaporization. The developed model was thus based on mass transfer through a boundary layer. The validation of this model in a dense fluidized bed justified its application to operating conditions were the bed voidance is higher. A detailed study of operating parameters will help determine how to improve vaporization. (author)

  20. Experimental study of the MHD activity associated to the mode m=2, n=1 in the Tore Supra tokamak; Etude experimentale de l`activite MHD associee au mode m=2, n=1 dans le tokamak Tore Supra

    Energy Technology Data Exchange (ETDEWEB)

    Turlur, S.


    In tokamaks such as Tore Supra, the plasma confinement magnetic structure can be severely affected when Magnetohydrodynamic (M.H.D.) instabilities are destabilized. Experimentally, these instabilities are detected as magnetic fluctuations with captors located against the inner wall of the vacuum vessel. Fourier analysis provides amplitude, frequency and wave numbers of magnetic modes. In case of fast or transient phenomena, the analysis of magnetic fluctuations is completed using the singular value decomposition. In this dissertation, these analysis techniques are used to study two specific examples of M.H.D. activity related to the m = 2, n = 1 mode. On Tore Supra, the onset of this mode have strong consequences on the stability of partially or fully non inductive discharges. A regular and persistent sawtooth-like regime is observed on the electronic temperature leading to a significant degradation of the central confinement. Heat exhaust and particle balance are also essential parameters to achieve stationary discharges. On Tore Supra, these are studied with the ergodic divertor which produces stochastic magnetic field lines at the plasma edge. For optimal operating conditions of the ergodic divertor, the growth of the m = 2, N = 1 mode can lead to sudden destruction of magnetic equilibrium. For both cases, understanding and characterization of mechanisms leading to the observed m = 2, n = 1 M.H.D. activity are fundamental to obtain stationary discharges. (author). 115 refs.

  1. Experimental study of mechanical behaviour of a clay-stone: application to nuclear waste disposals; Etude experimentale du comportement mecanique d'argiles raides: application au stockage de dechets radio-actifs

    Energy Technology Data Exchange (ETDEWEB)

    Chiarelli, A.S. [Laboratoire de Materiaux et Sciences des Constructions, UPRES JE 2200, IUP Genie Civil et Infrastructures, 95 - Cergy Pontoise (France); Shao, J.F. [Laboratoire de Mecanique de Lille, URA CNRS 1441, EUDIL, 59 - Villeneuve d' Ascq (France); Ledesert, B. [Laboratoire de Sedimentologie et Geodynamique, FRE 2255, 59 - Villeneuve d' Ascq (France); Hoteit, N. [Agence Nationale pour la Gestion des Dechets Nucleaires (ANDRA), 92 - Chatenay Malabry (France)


    A study of mechanical behaviour of deep argillaceous rocks from East of France, the 'argilites de l'Est' as a potential host rock for radioactive waste disposal studied by ANDRA, (french national radioactive waste management agency) is presented. Some uniaxial and triaxial compression tests with unloading-reloading cycles were realised on samples from three different depths. Important plastic strains associated to directional degradation of elastic properties show that the two principles strain mechanisms are plasticity and induced anisotropic damage. At microscopic scale, it is related to sliding of clay sheets and oriented microcracks. The influence of mineralogy is that brittle behaviour is more important with calcite while it decreases with clay. (authors)

  2. Experimental thermal study and numerical simulation of a composite solar wall. Optimization of the energetic performances; Etude thermique experimentale et simulation numerique d`un mur solaire composite. Optimisation des performances energetiques

    Energy Technology Data Exchange (ETDEWEB)

    Zalewski, L.


    The objective of this work is the analysis of a passive solar component: the composite solar wall, a building component, which includes an insulating panel located behind the massive wall. This panel has two vents located at the top and at the bottom, which allow the air to circulate from the room to the layer in contact with the back of the massive wall, where it is heated, and then back to the room. The solar energy is transferred to the building by conduction through the massive wall, and then by convection using a thermosyphon phenomenon. The monitoring of 2 solar houses in Verdun-Thierville (Meuse, France) has clearly shown, control issues of the air layer. The wall must be operated as autonomously as possible, to not be a constraint for the occupants and to get an optimization of the energy gains. To solve these problems, a composite solar wall prototype was erected in a test cell at Cadarache and tested in real operating conditions. This allows to use a more complete instrumentation, to have access more easily to the sensors and to study various configurations. The first experiments revealed an inverse thermosyphon phenomenon. To avoid this effect, two systems were designed, tested at Cadarache and then implemented in the walls at Verdun. (author) 77 refs.

  3. Experimental study of the iron - smectite system in the presence of a solution at 80 at 300 deg; Etude experimentale du systeme fer-smectite en presence de solution a 80 deg et 300 deg

    Energy Technology Data Exchange (ETDEWEB)

    Guillaume, D


    One of the concepts proposed for the storage of nuclear wastes consists in the use of several confining barriers placed between the waste and the biosphere. Vitrified wastes are placed in steel containers surrounded by compacted smectite (engineered barrier). The whole is placed in the geological deep site (geological barrier, granite or argilite). The prediction of the evolution of the performances of the engineered barrier (low permeability, high swelling and sorption capacities) necessitates the study of the long-term stability of its mineral constituents under the conditions of the repository. The aim is to determine the nature and the chemistry of potential newly-formed phases resulting from the temperature increase due to disintegration reactions of the wastes, the presence of fluids coming from the geological barrier, and from the liberation of iron due to the degradation of the containers. Natural systems (in particular geothermal systems and sedimentary basins) give information concerning the possible evolutions of clay minerals. However, the potential availability of metallic iron makes a particular case of the nuclear waste storage. The aim of this experimental study is to put to evidence the chemical and mineralogical transformations of the iron-clay system at 80 deg C and 300 deg C, in the presence of a solution, so as to predict the possible transformations of a bentonite placed in the deep storage conditions of radioactive wastes. Several sets of experiments were carried out to study the reactivity of the MX80 bentonite in the presence of a solution ([CI]=3.10{sup -2} molal, Na/Ca=5,5 and liquid/rock ratio of 10) in absence or in presence of iron (magnetite + hematite or iron + magnetite) at 80 deg C or 300 deg C, and over periods ranging from 1 day to 9 months. Run products were submitted to multi-technique and multi-scale characterisation: X-ray diffraction, scanning electron microscopy, transmission electron microscopy (HR imaging, EDS and EELS) transmission Moessbauer spectroscopy, ICP AES and ICP MS analyses of solutions. The EQ3/6 geochemical code was used to simulate the experiments carried out and compare with the evolutions of the system in the given conditions. A new method for the punctual determination of the valence of iron in clay minerals was developed, using electron energy loss spectroscopy (EELS) on TEM. The valence of iron in a sample is determined by measuring the chemical shift of the L{sub 3} edge of iron or from the decomposition of this edge. The acquisition with TEM of the valence of iron (EELS) and the elemental analysis (EDS) from the same micro-volume (resolution < 100 nm) enables to calculate structural formulae at the particle scale and to point out variations among a population of clay particles. Simulations carried out with the EQ3/6 chemical code enable the validation of the equilibrium compositions of the mineralogical assemblages observed after 9 months experiments. They also enable to point out different reaction kinetics as a function of the experimental system. Experiments carried out at 80 deg C show that whatever the experimental system, no significant evolution of the mineralogy and crystallo-chemistry of the clay sample is observed. At 300 deg C, the di-octahedral smectite transforms to high charge tri-octahedral smectite. This transformation is triggered in the presence of iron oxides (magnetite + hematite) and run samples are enriched in iron. In the presence of metallic iron and magnetite, this evolution is very fast and followed by the formation of Fe-chlorite (close to amesite composition). Experiments carried out with a metallic iron plate as the source of iron and placed in the middle of the experimental sample, enable to point out spatial zoning in the distribution of the newly-formed chlorites. Far from the iron plate an Fe-Mg-chlorite is formed, whereas it is almost pure Fe-chlorite at the contact with the plate and its formation is faster. (authors)

  4. Interactions between fluids and natural clay rich sediments: experimental study in conditions simulating radioactive wastes underground storage; Interactions entre fluides et sediments argileux naturels: etude experimentale dans des conditions simulant un stockage souterrain de dechets radioactifs

    Energy Technology Data Exchange (ETDEWEB)

    Roubeuf, V


    The behaviour of clay rich sediments, especially an argilite from Oxfordian of Haute-Marne, a siltite from Albian series of Marcoule (Gard) and a bentonite from Wyoming, were experimentally studied under physical-chemical conditions close of those of an underground radioactive waste storage. The several steps of the creation of the storage in deep formation were simulated experimentally, in particular: - the effect due to oxidation at ambient temperature and moisture degree related to the arrival of air in the gallery, was tested, especially the interaction between acid fluids generated at the micron-scale of the altered pyrite micro-site and the surrounding minerals of the sediment, - the alteration due to weathering (damping/drying cycles) to simulate the effect of a surface storage of the sediments, - and finally, water-rock interactions at 80 and 200 deg C, which reproduce the thermic stress induced by the deposit of type C radioactive containers (stage of re-hydration under thermic stress). The various simulations lead to rather similar behaviour of minerals in the sediment and solutions. Mineralogical, geochemical and crystallographic analyses show that most minerals in sediments are preserved with no evidence of mineral neo-formation. Nevertheless, the study by X-ray diffraction shows variations in the interlayer spacing in relation with modifications of the hydration states. Changes in the interlayer occupancy of the clays are due to cationic exchange of the sodium of the interlayer by the calcium issued from the dissolution of carbonate and gypsum dissolution. I/S like minerals crystal-chemistry generally display little changes in the tetrahedral and octahedral occupancy and a rather good stability of crystal structure. The cationic exchange capacity (CEC) of the clay sediment display un-significant variations: after the damping/drying cycles, the argilite of Haute-Marne has lost about 15 % of their bulk CEC and the effect of acid micro-environment at pyrite micro-site leads to an increase of the bulk CEC from 3 to 25%. Whatever their initial composition, the experimental solutions acquired a sulphate character in relation with the dissolution of sulphate minerals as gypsum and jarosite fanned during the oxidation of the sediments. Equilibrium between fluids and carbonates in the rock, constitute a predominant rock forming mineral in the studied sediments yield to an important and rapid buffer effect on solutions, which pH ranges between 7,5 and 8,5. The liquid/rock ratio (L/R) of the experience has an influence on the concentrations of the elements resulting from the dissolution of minerals present in the rock (sulphates from pyrites oxidation). Extrapolated ionic concentrations for low L/R ratio typical of the geological formation indicate that the fluid migrating through an oxidised sediment will be enriched in SO{sub 4}{sup 2-},K{sup +}, Ca{sup 2+} and Mg{sup 2+} and therefore significantly distinct from that inferred in the deep sedimentary formations. (author)

  5. Theoretical and experimental study of a modular accelerating structure of travelling waves sections for high gradient tests (MECCANO); Etude theorique et experimentale d`une structure acceleratrice a ondes progressives demontable pour des tests fort gradient (Structure dite ``MECCANO``)

    Energy Technology Data Exchange (ETDEWEB)

    Chanudet, M


    A modular system, MECCANO, has been developed at the Laboratoire de l`Accelerateur Lineaire d`Orsay to study the physical and technical phenomena of high electric fields in travelling waves structures in the context of future linear colliders which can reach TeV energies. The behaviour of the electric field inside the section MECCANO is considered from the theoretical point of view with numerical simulations and analytical representations and from the experimental side with low and high power measurements. An infinite and uniform structure is classically described by series of RLC resonant circuits. The basic RF properties of the fundamental mode are given. For a finite section, the matching of a forward or backward travelling wave of any phase advance per cell is also represented by means of RLC circuits. The variations of the reflection and transmission properties of the structure with frequency and a new procedure to match couplers have been modelled and experimentally verified. The electromagnetic behaviour of each cavity and of the whole structure have been studied, the fundamental and first high order modes have been simulated by 2D or 3D codes and measured at low power. The matching of the phase, the amplitude and the reflection level of the accelerator is described. This procedure is found to be extremely delicate due to the abrupt changes in the geometry of the cavities. The structure has been tested at fields superior to 150 MV/m. The behaviour of some materials and surface layers subject to high gradients are presented. (author) 46 refs.

  6. Turbulent precipitation of uranium oxalate in a vortex reactor - experimental study and modelling; Precipitation turbulente d'oxalate d'uranium en reacteur vortex - etude experimentale et modelisation

    Energy Technology Data Exchange (ETDEWEB)

    Sommer de Gelicourt, Y


    Industrial oxalic precipitation processed in an un-baffled magnetically stirred tank, the Vortex Reactor, has been studied with uranium simulating plutonium. Modelling precipitation requires a mixing model for the continuous liquid phase and the solution of population balance for the dispersed solid phase. Being chemical reaction influenced by the degree of mixing at molecular scale, that commercial CFD code does not resolve, a sub-grid scale model has been introduced: the finite mode probability density functions, and coupled with a model for the liquid energy spectrum. Evolution of the dispersed phase has been resolved by the quadrature method of moments, first used here with experimental nucleation and growth kinetics, and an aggregation kernel based on local shear rate. The promising abilities of this local approach, without any fitting constant, are strengthened by the similarity between experimental results and simulations. (author)

  7. Synthesis of zirconium by zirconium tetrachloride reduction by magnesio-thermia. Experimental study and modelling; Elaboration de zirconium par reduction de tetrachlorure de zirconium par magnesothermie. Etude experimentale et modelisation

    Energy Technology Data Exchange (ETDEWEB)

    Basin, N


    This work deals with the synthesis of zirconium. The ore is carbo-chlorinated to obtain the tetrachloride which is then purified by selective condensation and extractive distillation. Zirconium tetrachloride is then reduced by magnesium and the pseudo-alloy is obtained according to the global following reaction (Kroll process): ZrCl{sub 4} + 2 Mg = 2 MgCl{sub 2}. By thermodynamics, it has been shown that the volatilization of magnesium chloride and the formation of zirconium sub-chlorides are minimized when the combined effects of temperature and of dilution with argon are limited. With these conditions, the products, essentially zirconium and magnesium chloride, are obtained in equivalence ratio in the magnesio-thermia reaction. The global kinetics of the reduction process has been studied by a thermal gravimetric method. A thermo-balance device has been developed specially for this kinetics study. It runs under a controlled atmosphere and is coupled to a vapor tetrachloride feed unit. The transformation is modelled supposing that the zirconium and magnesium chloride formation result: 1)of the evaporation of magnesium from its liquid phase 2)of the transfer of magnesium and zirconium tetrachloride vapors towards the front of the reaction located in the gaseous phase 3)of the chemical reaction. In the studied conditions, the diffusion is supposed to be the limiting process. The influence of the following parameters: geometry of the reactive zone, temperature, scanning rate of the argon-zirconium tetrachloride mixture, composition of the argon-zirconium tetrachloride mixture has been experimentally studied and confronted with success to the model. (O.M.)

  8. Experimental study of the mechanisms of CO{sub 2} capture by calcium cycle under circulating fluidized bed conditions; Etude experimentale des mecanismes de capture du CO{sub 2} par cycle calcium en lit fluidise circulant

    Energy Technology Data Exchange (ETDEWEB)

    Hoteit, A


    The work undertaken in this Thesis in partnership with department R and D of ALSTOM Power Boilers, CEMEX and the ADEME, relates to the experimental study of various phenomena associated to CO{sub 2} capture under circulating fluidized bed conditions. The size of particles, temperature and the CO{sub 2} concentration have an influence on the limestone calcination reaction. The reaction of carbonation of lime is not total. During successive cycles of calcination/carbonation, the rate of carbonation obtained with hydrated lime is increasingly higher than that obtained with the lime. Under continuously reducing conditions, the decomposition of sulphates present in the bed ashes is not total. This decomposition is total under reduction/oxidation cycles. A modeling of calcination allowed to determine the intrinsic kinetic constants of calcination and carbonation. (author)

  9. An experimental study on mineral sequestration of CO{sub 2} in basics and ultra basics rocks; Etude experimentale des reactions de carbonatation minerale du CO{sub 2} dans les roches basiques et ultrabasiques

    Energy Technology Data Exchange (ETDEWEB)

    Dufaud, F


    The first part of the thesis is dedicated to dissolution data of siderite FeCO{sub 3} and magnetite Fe{sub 3}O{sub 4} which have been monitored in situ on the FAME beamline of the european synchrotron radiation facility in Grenoble. Iron in solution close to siderite single crystals is shown to be divalent hydrated. The small size of the experimentally investigated volume of solution (200 *400 micrometer and 3 mm height) allowed to work with single crystals in well defined geometries. No specific interaction was observed between iron (II) and dissolved inorganic carbon, suggesting that modelling siderite evolution under high CO{sub 2} pressures by using CO{sub 2}-less very acidic (pH 1-2) solutions is adequate. Using initial reaction rates, we get an activation energy for siderite dissolution of 62 kJ.mol{sup -1}, consistent with existing literature data. Such a value is suggestive of a mineral/solution interface mechanism.. Data from this study and from literature are consistent over a temperature range 25 C - 125 C and a pH range pH 1-7 with an empirical law: pk = pH + E{sub a}/(ln(10)*RT(K)) - log(S/V) - 10,5 where E{sub a} = 62 kJ.mol{sup -1} and S/V is the ratio between solid surface S and fluid volume V. A value of activation energy of 73.5 kJ.mol{sup -1} is obtained in the case of magnetite, also consistent with mineral/solution processes. The second and major part of the thesis work is the realization of analogical experiments for simulating carbonation of basic and ultra basic minerals. Experiments were carried out on consolidated rock cores at 90 C and 280 bar of CO{sub 2} (low temperature experiments) and on powders contained in metallic capsules at 400-500 C and 1000-1700 bars of CO{sub 2} (high temperature experiments). The rate of mineral storage of CO{sub 2} was defined as the molar ratio of solid carbonate formed over total CO{sub 2} injected. It is of about 1% in three months in low temperature experiments whereas it reaches several tens of percents per hour in high temperature experiments. In all cases, carbonation is shown to proceed according to dissolution/precipitation mechanisms. A quasi stoichiometric coupling is evidenced between carbonation and (proto) serpentinization in high-temperature experiments. Newly formed carbonates are mostly magnesite MgCO{sub 3} with Fe and Ca in solid solution. In low temperature samples, the silicates are covered with a thin silica layer and with carbonate spherules consisting of ankerite CaFe(CO{sub 3}){sub 2} - dolomite CaMg(CO{sub 3}){sub 2} - siderite cores surrounded by magnesite overgrowth. In CO{sub 2}-saturated water, peridotites are more reactive than serpentinite and basalts, in accordance with thermodynamic modelling whereas serpentinites are the most reactive in the supercritical CO{sub 2} phase, showing stronger reactivities in this latter phase than in CO{sub 2}-saturated water. In high temperature experiments, the rate of mineral storage is larger at 400 C than at 500 C, in agreement with thermodynamic modelling of the system. High water fugacities and high fluid salinities are shown to have a positive effect on mineral storage rates. Isotopic mass balance of carbon have evidenced that about 15% of the mineral storage consist of a reduced carbon phase, also identified by transmission electron microscopy as an ill-organized graphite phase. (author)

  10. Experimental study of X-ray emission in laser-cluster interaction; Etude experimentale de l'emission X issue de l'interaction laser-agregats

    Energy Technology Data Exchange (ETDEWEB)

    Caillaud, T


    Rare gas cluster jets are an intermediate medium between solid and gas targets. Laser-cluster jets interaction may generate a great number of energetic particles as X-rays, UV, high harmonics, ions, electrons and neutrons. To understand all the mechanisms involved in such an interaction we need to make a complete study of individual cluster response to an ultra-short laser pulse. We studied the laser interaction with our argon cluster gas jet, which is well characterized in cluster size and density, to enlarge the knowledge of this interaction. We measured absorption, heating and X-ray emission spectra versus laser parameters and clusters size ({approx} 15-30 nm). We show that there is a strong refraction effect on laser propagation due to the residual gas density. This effect was confirmed by laser propagation simulation with a cylindrical 2-dimensional particle code WAKE. The role played by refraction was to limit maximum laser intensity on the focal spot and to increase interaction volume. By this way, X-ray emission was observed with laser intensity not so far from the ionization threshold (few 10{sup 14}{sup -2}). We also studied plasma expansion both at cluster scale and focal volume scale and deduced the deposited energy distribution as a function of time. Thanks to a simple hydrodynamic model, we used these results to study cluster expansion. X-ray emission is then simulated by TRANSPEC code in order to reproduce X-ray spectra and duration. Those results revealed an extremely brief X-ray emission consistent with a preliminary measure by streak camera (on ps scale). (author)


    Directory of Open Access Journals (Sweden)



    Full Text Available Ce travail consiste à étudier expérimentalement l’influence de quelques machines-outils sur le comportement à l’usure des plaquettes en carbure métalliques. Les essais ont été réalisés dans des conditions opératoires proches de celles industrielles. Les machines-outils utilisées sont caractérisées par leurs rigidités. Le suivi de l’évolution de l’usure des plaquettes en fonction du temps (τ et des éléments du régime de coupe (v, f et d sur chaque machine a été établi conformément à la méthode de planification des expériences. Les résultats obtenus ont été traités, analysés et interprétés physiquement.

  12. Experimental study of the drying in dense fluidized beds of a synthetic mud coated with support particulates; Etude experimentale du sechage en lit fluidise dense d'une boue synthetique enrobee sur des particules supports

    Energy Technology Data Exchange (ETDEWEB)

    Gode, C.; Shakourzadeh, K. [Universite de Technologie de Compiegne, L.G.P.I., 60 (France)


    This article presents the results of an experimental study of a new drying process for muddy materials and based on the fluidization technique. The granular phase has been obtained by the coating with mud of a porous mineral support, inert and recyclable in the process. Activated porous particulates of alumina (2-5 mm) have been chosen because of their heat transfer properties. A first part of the experimental study concerns the drying process. The second part concerns the attrition and elutriation mechanisms. The experimental measurements have been performed with a laboratory fluidized column (internal diameter = 150 mm) and with a fluidized column (internal diameter = 400 mm) from a semi-industrial facility. The air velocity and the drying temperature are the main adjustment parameters. The working temperatures have been fixed between 20 and 150 deg. C. (J.S.)

  13. Experimental and numerical study of the strong interaction between wakes of cylindrical obstacles; Etude experimentale et numerique de l'interaction forte entre sillages d'obstacles cylindriques

    Energy Technology Data Exchange (ETDEWEB)

    Brun, Ch


    In the context of thermal-hydraulics of nuclear reactors, strong interaction between wakes is encountered in the bottom of reactor vessels where control and measurement rods of variable size and disposition interact with the overall wakes generated in these flow zones. This study deals with the strong interaction between two wakes developed downstream of two parallel cylinders with a small spacing. The analysis focusses on the effect of the Reynolds regime which controls the equilibrium between the inertia and viscosity forces of the fluid and influences the large scale behaviour of the flow with the development of hydrodynamic instabilities and turbulence. The document is organized as follows: the characteristic phenomena of wakes formation downstream of cylindrical obstacles are recalled in the first chapter (single cylinder, interaction between two tubes, case of a bundle of tubes perpendicular to the flow). The experimental setup (hydraulic loop, velocity and pressure measurement instrumentation) and the statistical procedures applied to the signals measured are detailed in chapters 2 and 3. Chapter 4 is devoted to the experimental study of the strong interaction between two tubes. Laser Doppler velocity measurements in the wakes close to cylinders and pressure measurements performed on tube walls are reported in this chapter. In chapter 5, a 2-D numerical simulation of two typical cases of interaction (Re = 1000 and Re = 5000) is performed. In the last chapter, a more complex application of strong interactions inside and downstream of a bunch of staggered tubes is analyzed experimentally for equivalent Reynolds regimes. (J.S.)

  14. Experimental study of the influence of partner structure in the fusion of the almost symmetrical systems; Etude experimentale de l`influence de la structure des partenaires dans la fusion de systemes presque symetriques

    Energy Technology Data Exchange (ETDEWEB)

    Stodel-Le Lay, Christelle [Lab. de Physique Corpusculaire, Caen Univ., 14 - Caen (France)


    The cross-sections for the formation of evaporation residues in {sup 70}Zn and {sup 86}Kr reactions with {sup 150}Nd and {sup 130,136}Xe isotopes were measured for excitation energies of the compound nuclei ({sup 216,220,222}Th) varied from 7 MeV to 70 MeV, at the linear accelerator UNILAC of the nuclear facility GSI, Darmstadt (Germany). After de-excitation by evaporation (xn,pxn and {alpha}xn), the residual nuclei are separated from the primary beam and from spurious reaction products by the velocity filter SHIP and implanted into a silicon localization detector. Their subsequent decay via alpha particles with characteristic energies allows us to identify them and to deduce their yields. Experimental fusion-evaporation excitation functions are compared with those leading to the same compound nuclei obtained with other projectile and target combinations and with those calculated with a code developed at GSI. This code allows us to evaluate the evolution of the fission probability as a function of the incident energy for each system. The variation of cross-sections and of the fusion probability is studied as a function of the macroscopic and microscopic variables of the partners. For the synthesis of super-heavy elements, these results demonstrate quantitatively the interest in using partners of fusion with closed shell structures and rich in neurons (the fusion cross-section increases by a factor of 9 for a complementary pair of neutrons). On the other hand, closed shell compound nuclei do not influence the fusion cross-section. It will be worth synthesizing isotopes approaching the predicted stability region, nuclei with Z greater than 110 using neutron rich projectiles coming from secondary beams. (author) 104 refs., 71 figs., 11 tabs.

  15. Experimental investigation of the effects of aqueous species on the dissolution kinetics of R7T7 glass; Etude experimentale de l`influence d`especes aqueuses sur la cinetique de dissolution du verre nucleaire R7T7

    Energy Technology Data Exchange (ETDEWEB)

    Gin, S.


    This contribution to the study of aqueous corrosion of the French ``R7T7`` reference nuclear containment glass includes a bibliographic survey of prior investigations, highlighting the problems encountered in interpreting the interactions in systems containing clay materials in contact with the glass. An experimental methodology is proposed to investigate the effects of inorganic aqueous species separately from those of a few organic acids on the dissolution mechanisms and kinetics of R7T7 glass at 90 deg. C. The experimental results discussed support the idea that several glass network forming elements may have a kinetically limiting role. The most likely hypothesis to account for the absence of saturation conditions with respect to the glass in certain clay media involves the formation of complexes with kinetically limiting metallic elements such as aluminum released by glass corrosion. This work contributes to a better understanding of the basic mechanisms of nuclear glass dissolution in a geological repository environment. It facilitates the interpretation of glass alteration studies in realistic or actual solutions and may contribute to specifying near field chemical barriers in the form of additives (amorphous silica, aluminum hydroxides or phosphates) around the glass disposal package to enhance the stability of the glass matrix. (author). 148 refs., 40 figs., 32 tabs., 1 append.

  16. Experimental studies of ions and atoms interaction with insulating surface; Etude experimentale de l'interaction rasante d'atomes et d'ions sur des surfaces isolantes

    Energy Technology Data Exchange (ETDEWEB)

    Villette, J


    Grazing collisions (<3 deg.) of keV ions and atoms: H{sup +}, Ne{sup +}, Ne{sup 0}, Na{sup +} on LiF (001) single crystal, an ionic insulator, are investigated by a time of flight technique. The incident beam is chopped and the scattered particles are collected on a position sensitive detector providing differential cross section while the time of flight gives the energy loss. Deflection plates allow the charge state analysis. Secondary electrons are detected in coincidence allowing direct measurements of electron emission yield, angular and energetic distribution through time of flight measurements. The target electronic structure characterized by a large band gap, governs the collisional processes: charge exchange, electronic excitations and electron emission. In particular, these studies show that the population of local target excitations surface excitons is the major contribution to the kinetic energy transfer (stopping power). Auger neutralization of Ne{sup +} and He{sup +} ions reveals the population of quasi-molecular excitons, an exciton bound on two holes. Referenced in the literature as trion. A direct energy balance determines the binding energy associated with these excited states of the surface. Besides these electronic energy loss processes, two nuclear energy loss mechanisms are characterized. These processes imply momentum transfer to individual target atoms during close binary collisions or, if the projectile is charged, to collective mode of optical phonons induced by the projectile coulomb field. The effect of the temperature on the scattering profile, the contribution of topological surface defects to the energy loss profile and to skipping motion on the surface are analyzed in view of classical trajectory simulations. (author)

  17. Experimental study of the aluminum droplet combustion under forced convection. Influence of the gaseous atmosphere; Etude experimentale de la combustion des gouttes d'aluminium en convection forcee. Influence de l'atmosphere gazeuse

    Energy Technology Data Exchange (ETDEWEB)

    Sarou-Kanian, V.


    Because of its high energetic power, the combustion of aluminum particles in solid propellant rocket motors improves the efficiency of heavy-lift launcher as Ariane 5. Aluminum particles burn in a gaseous atmosphere essentially composed of H{sub 2}O, CO{sub 2}, N{sub 2}, HCl, H{sub 2}, and CO, at high pressure (P=60-70 atm) and high temperature (T>3000 K). In the present work, we have been particularly interested in the influence of the gaseous atmosphere on the different burning processes both in the gas-phase and at the aluminum droplet surface. An experimental set-up was developed in order to describe precisely, thanks to several analysis techniques (high-speed camera, pyrometry, spectrometry, SEM, nuclear activation) the combustion of aerodynamically levitated millimetric aluminum droplets in gas mixtures with compositions close to the propellant ones (H{sub 2}O, CO{sub 2}, N{sub 2}). The main result is that each species plays a different role in the aluminum combustion. The water vapor has the biggest influence in the gas-phase process due to the production of hydrogen facilitating the heat and mass diffusion between the flame and the droplet. Nitrogen is essentially acting in surface reactions with the formation of aluminum nitride (AlN) and oxynitride (AlON) which may completely cover the droplet and stop the gas-phase combustion. Carbon dioxide has a double effect. On the one hand, CO{sub 2} burns in the flame, but it is less efficient than H{sub 2}O because the heat and mass transfer properties are poorer for CO than for H{sub 2}. On the other hand, a carbon dissolution phenomenon occurs in the aluminum droplet during burning which may reach saturation (20-25% molar) and involves a carbon rejection at the surface leading to the end of the gas-phase combustion. (author)

  18. Double protection passive pour les equipages de l’helicoptere d’attaque Tigre : Concept et etude experimentale (Design and Experimental Study of the Passive Double Hearing Protection for the Crew of the Attack Helicopter Tiger) (United States)


    bourdonnements observés lors de vols similaires réalisés sans bouchons. D’où un confort acoustique après le vol qui n’est pas du tout négligeable. façon empirique, des bouchons de type “ mousses Quies ” ont été sélectionnés pour leur port confortable et leur forte atténuation dès les basses...Protection aux impacts 475 g 700 g Protection acoustique 75 g 200 g Communication 80 g 120 g Liaisons mécaniques 120 g 220 g Dans le viseur de casque

  19. Determination of the turbulent viscosity inside a strongly heated rectangular jet: experimental and numerical studies; Determination de la viscosite turbulente dans un jet rectangulaire fortement chauffe: etudes experimentale et numerique

    Energy Technology Data Exchange (ETDEWEB)

    Sarh, B.; Gokalp, I.; Sanders, H. [Centre National de la Recherche Scientifique (CNRS), 45 - Orleans-la-Source (France)


    In the framework of the studies carried out by the LCSR on variable density flows and diffusion turbulent flames, this paper deals with the study of the influence of density variation on the characteristics of a heated rectangular turbulent jet emerging in a stagnant surrounding atmosphere and more particularly on the determination of turbulent viscosity. The dynamical field is measured using laser-Doppler anemometry while the thermal field is measured using cold wire anemometry. A numerical predetermination of the characteristics of this jet, based on a k-{epsilon} modeling, is carried out. (J.S.) 6 refs.

  20. Numerical and experimental study of the beam dynamics of CANDELA photo-injector and associated instrumentation; Etude numerique et experimentale de la dynamique du faisceau du photo-injecteur CANDELA et instrumentation associee

    Energy Technology Data Exchange (ETDEWEB)

    Devanz, Guillaume [Paris-6 Univ., 95 Paris (France)


    Laser triggered radiofrequency guns are the most luminous electron sources allowing to reach the performances requested by highly demanding applications like the e{sup +}/e{sup -}linear colliders and the short wave free electron lasers. CANDELA is a band S photo-injector triggered by a sub-picosecond laser. It allows reaching peak currents of hundred of amperes at average energies higher than 2 MeV. The original concept of two accelerating cavities aims at minimizing the transverse and longitudinal emittances following the Gao's principles. From practical reasons the operating parameters, particularly the laser pulse duration, do not correspond to those considered in the design. Hence, numerical simulations were performed to evaluate the gun's performances in experimental environment. The study of a stabile injector operation resulted in evolutions with consequences in the phase control systems implying the laser and the HF (Hyper Frequency) source. The beam transverse and longitudinal characteristics have been measured as a function of the main parameters i.e., the beam charge and the phase shift between the laser and the HF wave. Measurements of the transverse emittance energy dispersion and wave packed duration are presented for several injector configurations. The systems of existing beam measurements have been studied to determine the resolution and the experimental conditions to fulfill, in order to suggest improvements for the CANDELA beam. The experiments with the beam have been compared with numerical simulations. Agreement was obtained within wide ranges of parameters for most of the characteristic beam quantities.

  1. Experimental study of an oxygen-hydrogen diffusion flame laden with solid alumina particles; Etude experimentale d'une flamme de diffusion oxygene-hydrogene ensemencee en particules solides d'alumine

    Energy Technology Data Exchange (ETDEWEB)

    Labor, S.


    Monocrystalline sapphire microspheres are generated through the melting of alumina (AL{sub 2}O{sub 3}) particles in a flame. The alumina particles are injected in a very peculiar O{sub 2}/H{sub 2} confined diffusion flame as it is a downwards vertical flame having fuel in periphery of a central powdered oxygen jet. Quantitative measurements were carried out (ADL, PIV) and supplemented by a numerical study (N3S-Natur). (1) The laminar behavior of the isothermal conditions is kept through reactive flow. Therefore, particles will mainly collide due to speed gradients. (2) It has been shown that an axial particle will have a transit time int the high temperature zone very different to that of an off-line one. (3) The PIV date proved that the particle density was not homogeneous. (4) The hydrogen jet hardly influences the flame aerodynamic structure. Conversely, the central oxygen jet is at premium due to its effect on both the flame speed and temperature distribution. (author)

  2. Double protection passive pour les equipages de l'helicoptere d'attaque "Tigre" : Concept et etude experimentale (Design and Experimental Study of the Passive Double Hearing Protection for the Crew of the Attack Helicopter "Tiger")

    National Research Council Canada - National Science Library

    Baudou, J; Reynaud, G; Poussin, G; Leger, A


    ... naturellement imposee comme axe de travail. Prealablement evalues de facon empirique, des bouchons de type "mousses Quies" ont ete selectionnes pour leur port confortable et leur forte attenuation...

  3. Strontium and caesium transport in unsaturated soil from Chernobyl Pilot Site under steady flow conditions; Transfert de radioelements en zone non saturee. Etude experimentale et modelisation appliquees au Site Pilote de Tchernobyl

    Energy Technology Data Exchange (ETDEWEB)

    Szenknect, St


    This work is devoted to the quantification and the identification of the predominant processes involved in strontium and caesium transport in unsaturated soil from Chernobyl Pilot Site under steady flow conditions. The transport and fate of radionuclides in the subsurface is affected by various physical and chemical processes including advective and diffusive transport as well as chemical and biological transformations. Laboratory experiments and the use of a multiple tracer approach allow to isolate the contributions of each elementary process and to control the physico-chemical conditions in the system. To be more representative of the field conditions, we decided to perform column miscible displacement experiments. We perform batch and flow-through reactor experiments to characterize the radionuclides sorption mechanisms. Miscible displacement experiments within homogeneous columns and modeling allow to characterize the hydrodynamic properties of the soil and to describe the radionuclides behaviour under dynamic conditions at different water contents. We show that the water content of porous media affect the transport behaviour of inert and strongly sorbing radionuclides. Our results demonstrate that a parametrized transport model that was calibrated under completely saturated conditions was not able to describe the advective-dispersive transport of reactive solutes under unsaturated steady state conditions. Under our experimental conditions, there is no effect of a decrease of the mean water content on the sorption model parameters, but the transport parameters are modified. We established for the studied soil the relation between hydrodynamic dispersion and water content and the relation between pore water velocity and water content. (author)

  4. Numerical and experimental study of the mixture of engine jets in the wake vortices of an airline aircraft; Etude numerique et experimentale du melange des jets de moteur dans les tourbillons de sillage d'un avion de ligne

    Energy Technology Data Exchange (ETDEWEB)

    Brunet, St.


    This study is a contribution to the understanding of the formation and duration of aircraft condensation trails. The development of a numerical code based on the direct resolution of the 3-D compressible Navier-Stokes equations has been done first. Then, an experiment has been carried out in a wind tunnel to analyze the problem of the mixture of heated jets in a wing wake. A first validation of the numerical method has been carried out from bibliographic results and measurements of the mixture evolution of an inert tracer contained in the engine jets during a flight test. In order to characterize the condensation inside the wake, the evolution of the local water vapor saturation ratio has been calculated. The influence of the Crow instability on the mixture of effluents in the high atmosphere is also shown. Finally, a comparison is made between the numerical simulation results and the experimental measurements obtained in this study. The numerical results have also permitted to characterize the low scale exchange mechanisms between a turbulent jet and a swirl flow. (J.S.)

  5. Experimental and numerical study of two-phase flows at the inlet of evaporators in vapour compression cycles; Etude experimentale et numerique d'ecoulements diphasiques a l'entree des evaporateurs de cycles thermodynamiques

    Energy Technology Data Exchange (ETDEWEB)

    Ahmad, M


    Maldistribution of liquid-vapour two phase flows causes a significant decrease of the thermal and hydraulic performance of evaporators in thermodynamic vapour compression cycles. A first experimental installation was used to visualize the two phase flow evolution between the expansion valve and the evaporator inlet. A second experimental set-up simulating a compact heat exchanger has been designed to identify the functional and geometrical parameters creating the best distribution of the two phases in the different channels. An analysis and a comprehension of the relation between the geometrical and functional parameters with the flow pattern inside the header and the two phase distribution, has been established. A numerical simulations of a stratified flow and a stratified jet flow have been carried out using two CFD codes: FLUENT and NEPTUNE. In the case of a fragmented jet configuration, a global definition of the interfacial area concentration for a separated phases and dispersed phases flow has been established and a model calculating the fragmented mass fraction has been developed. (author)

  6. Experimental study of a separated jets burner: application to the natural gas-pure oxygen combustion; Etude experimentale du comportement de bruleurs a jets separes: application a la combustion gaz naturel-oxygene pur

    Energy Technology Data Exchange (ETDEWEB)

    Salentey, L.


    The evolution of pollution standards and the optimisation of furnaces performances require a development of new burner generation and also the improvement of combustion techniques. Actually, the use of oxy-combustion in separated jets burners offers interesting prospects for NO{sub x} emission reduction and on the modularity of flames properties (lift off, flame front topology, flame length). The complex geometry of those burners leads to several problems like the three-dimensional character of the flow, which may sometimes disturb the flame stability as flames are lifted above the burner. This experimental study deals with a simplified version of that kind of burner constituted with a central natural gas jet surrounded by two oxygen jets. Primary, the study of non-reactive jet was planned in order to understand dynamic and mixture phenomena involved between jets and to provide a database useful for the computer code validation. The reactive flow developed in a furnace, which simulates the real conditions, had been characterised. The studies of the dynamic field using Laser Doppler Velocimetry (LDV) and of the turbulent mixture by conditional Laser tomography were supplemented in combustion by the visualisation of the spontaneous emission of radical OH, in the initial and final zone of the oxy-flames, like by the measurement of pollutants like NO{sub x} and soot. The measurements carried out while varying speeds of injection as well as the gap between the jets made possible the highlight of the influence of these parameters upon the stabilisation of the oxy-flames as well as the modification of the topology and the characteristics of the flows. The comparison of the measurements made in non-reactive and reactive flow shows the influence of oxy-combustion on the dynamic and scalar development of the flow for this type of burners. (author)

  7. Transient behaviour of an adsorption refrigerator. Experimental study of the alcohol/activated carbon system; Comportement transitoire d`une machine frigorifique a absorption. Etude experimentale du systeme alcool / charbon actif

    Energy Technology Data Exchange (ETDEWEB)

    Luo, L. [Centre National de la Recherche Scientifique (CNRS), 54 - Nancy (France). Lab. des sciences du genie chimique; Feidt, M. [Nancy-1 Univ., 54 - Vandoeuvre-les-Nancy (France)


    The objective of this article is to present an experimental study of an adsorption refrigerator, accounting for the transient phenomena in each component of the machine, in particular in the adsorber. The couples adsorbate/adsorbent investigated (activated carbon/methanol or ethanol) have been chosen on the basis of an earlier comparative study. We have performed a full series of experiments to investigate the transient evolution of temperatures, pressures and the cycled mass, in a discontinuous cycle, and along the components of the refrigerating machine. The influence of various parameters is studies by varying one at a time: adsorbate/ adsorbent couple, alcohol load in adsorber, alcohol flowrate, heating power, initial desorption temperature. All these experimental observation are discussed and compared to transient models developed in a separate article. (authors) 12 refs.

  8. Modeling and experimental study of heat transfer in innovate building components for industrial production; Modelisation et etude experimentale des echanges de chaleur dans des composants innovants de batiment industriels

    Energy Technology Data Exchange (ETDEWEB)

    Lacena-Neildez, A.


    The main objective of the thesis is the study of a new roof thermal behaviour with the thermal transfer by radiation and convection in order to propose an alternative to the mechanical air-conditioning in the overseas islands, and to determine the way of improving the energy performance. Two fundamental results were obtained. First, the coating has a great importance in relation with the radiative properties. Secondly, air channel geometries can complete insufficient coatings in their cooling purpose. In this thesis, both experimental and modelling analyses were carried out. A solar simulator was used for the experiment to carry out a comparative study of prototypes. A performance indicator was defined; it is the eliminated flux, because the temperature is not sufficient to describe the heat exchange. At the same time, the radiative properties of coatings were measured and a characterisation of paints allows to start a new research of innovative infrared reflective paints. A mono-dimensional model was developed describing thermal exchanges in an air channel component. A sensibility study was carried out which allows to determine the main parameters. The experimental study validated the model in rear flux, more realistic than stagnation. Finally, the feasibility of this new steel component was concluded by a technical-economical study. (author)

  9. E. P. R. spectroscopic study of nitroxide mono- and bi-radicals; Etude par spectroscopie de resonance paramagnetique electronique de monoradicaux et de biradicaux nitroxydes

    Energy Technology Data Exchange (ETDEWEB)

    Lemaire, H. [Commissariat a l' Energie Atomique, Grenoble, 38 (France). Centre d' Etudes Nucleaires


    experimentales d'etude de produits parfaitement bien definis au point de vue structure chimique. L'etude par resonance paramagnetique electronique de monoradicaux nitroxydes conduit a la determination des axes et des elements du tenseur d'interaction hyperfine electron-noyau d'azote et du tenseur d'anisotropie du facteur de Lande: les resultats sont relies a la structure electronique des radicaux. On interprete qualitativement l'influence du solvant sur les elements des tenseurs, ainsi que les elargissements selectifs des raies hyperfines en milieu visqueux. Dans les biradicaux nitroxydes, la structure hyperfine depend non seulement des interactions magnetiques propres a chaque monomere, mais egalement de la grandeur de l'interaction d'echange separant les etats singulet et triplet du dimere; les biradicaux etudies ici sont les premiers composes organiques pour lesquels l'influence de l'echange sur la structure hyperfine est clairement mise en evidence. La presence de deux electrons non apparies introduit egalement une interaction magnetique dipolaire electron-electron, que l'on peut mettre a profit, dans le cas d'un echange intermediaire, pour determiner le signe de l'echange en etudiant le biradical dans un cristal liquide. Ces composes presentent egalement des elargissements selectifs de transitions hyperfines lorsque la viscosite du solvant croit. La theorie utilisee pour interpreter les largeurs de raie des monoradicaux est etendue au cas des biradicaux; elle rend compte des resultats experimentaux si l'on introduit simultanement les anisotropies des facteurs de Lande et des interactions dipolaires electrons-noyaux et electron-electron, en conduisant a une autre determination du signe de l'echange. (auteur)

  10. Bacterial mitosis

    DEFF Research Database (Denmark)

    Møller-Jensen, Jakob; Borch, Jonas; Dam, Mette


    Bacterial DNA segregation takes place in an active and ordered fashion. In the case of Escherichia coli plasmid R1, the partitioning system (par) separates paired plasmid copies and moves them to opposite cell poles. Here we address the mechanism by which the three components of the R1 par system...... movement is powered by insertional polymerization of ParM. Consistently, we find that segregating plasmids are positioned at the ends of extending ParM filaments. Thus, the process of R1 plasmid segregation in E. coli appears to be mechanistically analogous to the actin-based motility operating...

  11. Etude de la distribution des champs magnetostatiques dans une section d'essais de type CANDU chauffee par effet de joule (United States)

    Hall, Darren

    Parmi tous les parametres physiques consideres lors de l'analyse de surete d'un reacteur nucleaire, le flux de chaleur critique (FCC) fait partie des plus importants. Afin d'etudier le FCC sous des conditions experimentales relativement securitaires, la chaleur degagee par le combustible nucleaire peut etre reproduite grâce a l'effetet de Joule. Or, afin de generer la puissance thermique necessaire pour les experiences sur le FCC des reacteurs CANDU, des courants electriques tres eleves de l'ordre de plusieurs dizaines de milliers d'amperes sont requis ce qui genere un champ magnetostatique tres intense capable d'interagir avec la section d'essais et l'instrumentation. Consequemment, l'industrie nucleaire est preoccupee par les effets de ce champ magnetique sur les donnees collectees et par les forces d'origine magnetique agissant sur le systeme. Dans ce contexte, le travail presente dans ce memoire de maitrise vise l'implementation d'une methodologie de calcul capable de fournir une information detaillee sur le champ magnetique engendre par ces courants. Toutefois, etant donne la complexitee du systeme, une etude magnetostatique appropriee requiert une methode numerique afin d'obtenir une distribution fiable du champ magnetique a travers differentes structures de la section d'essais. a cet effet, nous considerons dans le cadre de ce memoire le volume integral equation method (VIEM), une methode bien etablie basee sur les solutions integrales des equations de Maxwell. Plus precisement, nous employons le VIEM afin d'etudier la distribution du champ magetique a l'interieur de la section d'essais et pour determiner l'influence des prises concues pour le branchement des capteurs de pression sur la symetrie de cette distribution. Etant donne que les methodes integrales incluent les conditions aux limites et par consequent ne requierent pas l'emploi d'un critere de convergence, nous proposons une nouvelle technique de discretisation. L'emploi de cette procedure nous

  12. Estadísticos poblacionales de Triatoma sordida Stäl 1859 (Hemiptera : Reduviidae en condiciones experimentales

    Directory of Open Access Journals (Sweden)

    Oscherov Elena B.


    Full Text Available Se realizó el seguimiento longitudinal de cuatro cohortes de 100 huevos cada una. Los insectos fueron criados a 28°C ± 3°C y 63 % ± 10% de humedad relativa, y alimentados cada siete días sobre gallina (Gallus domesticus, durante 40 min. Las cohortes fueron controladas una vez por semana. Se observó un valor constante de supervivencia en las cuatro cohortes, sin picos importantes. La expectativa de vida de los machos, en promedio, fue de 37,4 semanas y la de las hembras 36,8. El tiempo generacional demandó 61,7 semanas. La tasa reproductiva neta indica que esta población se incrementará 143,3 veces con cada generación. La tasa intrínseca de crecimiento natural fue de 0,082 por semana. El máximo valor reproductivo se verificó, en promedio, a la tercer semana del ingreso al estado adulto. La distribución estable de edades de T. sordida sería de 32% huevos, 26% ninfas de primer estadio, 19% ninfas de segundo estadio, 13% ninfas de tercer estadio, 6% ninfas de cuarto estadio, 3% ninfas de quinto estadio y 1% de adultos. Es la primera cita para Argentina sobre la tabla de vida de T. sordida. El análisis de estos parámetros permite considerar que esta especie se comporta como "K estratega", en condiciones experimentales.

  13. Etude comparative de deux produits de neem (huile et poudre) sur ...

    African Journals Online (AJOL)

    Etude comparative de deux produits de neem (huile et poudre) sur les stades préimaginaux du moustique Culex quinquefasciatus(Diptera : Culicidae) ... for nymphs only contact effects are showed. Neem oil formulated 1 % appears more effective than neem powder 0.3 % for mosquitoes control in aquatic environment.

  14. Etude morphologique d'un cas de femme XY avec dysgénésie ...

    African Journals Online (AJOL)

    Mots clés : Etude morphologique, femme XY, dysgénésie gonadique pure. The authors describe the morphological features of a twenty years old female, without ambiguous genitalia, presenting a 46,XY karyotype, normal female external genitalia and gonadal dysgenesis. She has an android type of morpho dysplasia and ...

  15. English Department Reforms at the Hautes Etudes Commerciales: Entering the Nineties. (United States)

    Shanahan, Daniel


    Discusses the reforms made in the fall of 1990 in the English department at the Ecole Hautes Etudes Commerciales (Paris, France), which were based on the basis of a combination of literature-culture and second- and foreign-language acquisition pedagogical biases. (four references) (VWL)

  16. Contribution a l'etude des plantes alimentaires mineures dans la ...

    African Journals Online (AJOL)

    Contribution a l'etude des plantes alimentaires mineures dans la prefecture de Dankpen (Togo). AB Kebenzikato, S Akpavi, K Batawila, K Wala, M Dourma, K Kossi-Titrikou, KS Amouzou, I Butare, H Dantsey-Barry, K Akpagana ...

  17. 152 Etude de cas sur l'impact des amendements organiques vis-à ...

    African Journals Online (AJOL)

    john mukalay

    Etude de cas sur l'impact des amendements organiques vis-à-vis de la salinité en culture de bananier. Michel Mazinga KWEY1, Séverin Kalala BANZE2 et John Banza MUKALAY2*. 1Laboratoire des cultures in vitro, Département de phytotechnie, Faculté des sciences agronomiques,. Université de Lubumbashi, BP 1825, ...

  18. Etude de l'influence des modes de transformation sur les teneurs en ...

    African Journals Online (AJOL)

    Etude de l'influence des modes de transformation sur les teneurs en lycopène de quatre variétés de tomates de la région du nord du Burkina Faso. I Sawadogo, M Koala, C Dabire, LP Ouattara, VBEJT Bazie, A Hema, C Gnoula, E Pale, RHC Nebie ...

  19. Etude comparée des paramètres agromorphologiques de dix (10 ...

    African Journals Online (AJOL)

    Etude comparée des paramètres agromorphologiques de dix (10) variétés de patate douce ( Ipomoea batatas (L.) Lam cultivées au champ dans deux (2) conditions climatiques au Tchad et au Burkina Faso.

  20. Etude comparative de la flore aux abords des cours d'eau dans les ...

    African Journals Online (AJOL)

    Etude comparative de la flore aux abords des cours d'eau dans les zones hypo et hyper endémiques d'ulcère de Buruli en Côte d'Ivoire. C. Cisse Boni, E. Ehouman, D. Soro, M.W. Kone, A. Bakayoko, F. Dembele, K. Bauthire, M Dosso ...

  1. SEPA (Service d'etudes, de Procedes et Analyses)

    International Nuclear Information System (INIS)


    The SEPA (Service d'etudes, de Procedes et Analyses), is an engineering department specializing in the analysis and processing of uranium ore. It comes from a long tradition of mining in France that began sixty-five years ago with the French Atomic Energy Commission (CEA) and that has been carried on by COGEMA and then Areva. SEPA brings a combination of experience and excellence to its activities thanks to its multidisciplinary staff (mineralogists, chemists, mechanical engineers, instrumentation specialists...), who come from diverse cultural backgrounds. Today, the SEPA draws on its wealth of know-how and expertise to devise and develop new methods for processing and valorizing ores everywhere in the world. Its mission is to: - Develop and conduct laboratory tests on methods for processing uranium and other valuable elements such as molybdenum, vanadium, rare earths, niobium, tantalum and thorium; - Analyze ores obtained during prospecting at locations all over the world; -Serve as an advisor in the commissioning of Areva industrial units and ore processing plants; -Perform environmental analyses during the full period of mining operations; -Conduct audits and provide advice on processes and analysis methods; -Carry out research and development in all aspects of minerals treatment (including the filing of patents). The SEPA combines experience, skills and a global approach to technologies used in ore processing. Its role is to continually improve the processes for extracting, processing and valorizing ores while protecting the environment

  2. Patrones iniciales de sucesión en claros experimentales de plantaciones de Pinus patula en el Embalse de Chisacá, Usme, Cundinamarca (Colombia)


    Vargas Ríos Orlando; Corredor Velandia Samanta


    Se presenta un estudio que determina el efecto de la creación de claros experimentales con diferentes densidades sobre los patrones iniciales de sucesión dentro de plantaciones de Pinus patula. Adicionalmente, se evaluó el efecto de la remoción de acículas del suelo. Antes del establecimiento de los claros ya se encontraban en las parcelas la mayoría de las especies (30), pero en muy baja proporción; luego del aclareo, se registraron
    25 especies determinadas entre plantas vasculare...

  3. Patrones iniciales de sucesión en claros experimentales de plantaciones de Pinus patula en el Embalse de Chisacá, Usme, Cundinamarca (Colombia)


    Samanta Corredor Velandia; Orlando Vargas Ríos


    Se presenta un estudio que determina el efecto de la creación de claros experimentales con diferentes densidades sobre los patrones iniciales de sucesión dentro de plantaciones de Pinus patula. Adicionalmente, se evaluó el efecto de la remoción de acículas del suelo. Antes del establecimiento de los claros ya se encontraban en las parcelas la mayoría de las especies (30), pero en muy baja proporción; luego del aclareo, se registraron 25 especies determinadas entre plantas vasculares (20) y...

  4. Modelización del sistema de almacenamietno de energía y de su regulador de un vehículo solar de fines experimentales


    Mendoza Zaplana, Sergio


    En este proyecto, se pretende modelizar con el programa ‘Matlab-Simulink’ un sistema de acumulación y regulación para un vehículo solar de fines experimentales. Compuesto este por un motor de corriente continua de imanes permanentes de características similares a las que presenta el prototipo del vehículo Aníbal, desarrollado por el Departamento de Ingeniería Eléctrica de la Universidad Politécnica de Cartagena. Con el objetivo de modelizar un sistema de acumulación y regulación además de ave...

  5. Efecto inmunomodulador de la melatonina en modelos experimentales de la infección por el virus de encefalitis equina venezolana


    Valero, Nereida


    De este trabajo experimental de análisis in vivo e in Vitro del efecto inmunomodulador de la melatonina en modelos experimentales de infección por el EEV, se deducen las siguientes conclusiones: 1.El tratamiento preventivo y mantenido con MLT ejerce un efecto protector en ratones infectados por el virus de EEV, aumentando la supervivencia. 2.El tratamiento con MLT induce un efecto inmunomodulador sobre los animales infectados con EEV que se caracteriza por un incremento de los niveles citocin...

  6. Efecto de la ración alimenticia en el crecimiento de juveniles de tilapia Oreochromis aureus (Steindachner) bajo condiciones experimentales de cultivo


    M. García-Ulloa


    Se evaluó el crecimiento de juveniles (2.77 g de peso promedio) de la tilapia Oreochromis aureus (Steindachner), cultivados con diferentes raciones alimenticias bajo condiciones experimentales de laboratorio. Los tratamientos consistieron en el suplemento alimenticio de 6, 8 y 10% del peso de la biomasa total, los cuales fueron estudiados por triplicado en peceras de vidrio de 113 L de capacidad, ajustando una densidad inicial de 25 peces por acuario. El ensayo tuvo una duración de 35 días, y...


    Directory of Open Access Journals (Sweden)

    Marina Dinic


    Full Text Available Plasmids, extrachromosomal DNA, were identified in bacteria pertaining to family of Enterobacteriacae for the very first time. After that, they were discovered in almost every single observed strain. The structure of plasmids is made of circular double chain DNA molecules which are replicated autonomously in a host cell. Their length may vary from few up to several hundred kilobase (kb. Among the bacteria, plasmids are mostly transferred horizontally by conjugation process. Plasmid replication process can be divided into three stages: initiation, elongation, and termination. The process involves DNA helicase I, DNA gyrase, DNA polymerase III, endonuclease, and ligase.Plasmids contain genes essential for plasmid function and their preservation in a host cell (the beginning and the control of replication. Some of them possess genes whichcontrol plasmid stability. There is a common opinion that plasmids are unnecessary fora growth of bacterial population and their vital functions; thus, in many cases they can be taken up or kicked out with no lethal effects to a plasmid host cell. However,there are numerous biological functions of bacteria related to plasmids. Plasmids identification and classification are based upon their genetic features which are presented permanently in all of them, and these are: abilities to preserve themselves in a host cell and to control a replication process. In this way, plasmids classification among incompatibility groups is performed. The method of replicon typing, which is based on genotype and not on phenotype characteristics, has the same results as in compatibility grouping.

  8. Browse Title Index

    African Journals Online (AJOL)

    Z. DIBI, A BOUKABACHE, P. PONS. Vol 17 (2005), Etude experimentale des capteurs solaires a air: le sechage de la figue, Abstract. A Abene, V Dubois, A Ouagued, M Si-Youcef, M Le Ray. Vol 16 (2003), Etude experimentale des performances thermiques de diverses configurations de chicanes placees dans la veine d' ...

  9. Programa de viviendas experimentales en Móstoles, Comunidad de Madrid. España. (Preámbulo

    Directory of Open Access Journals (Sweden)

    Abad Carrascosa, Manuel


    Full Text Available This program consists of a total of 102 houses. They are situated in one of the housing estates in the General Urbanization Plan of the Móstoles Town Council (Town on outskirts of Madrid. The development of the prototypes of three different types of housing is to be studied with the aim of studying the experimental results of each one: — Progressive housing. — Housing with passive solar energy — Housing constructed on a basis of a special system in series. The three have the common aim of achieving finanical saving, but by different means: initial investment, saving in energy expenditure, the constructive system. The group of houses is about to be delivered into the hands of its users. We regret the incompleteness of the Information due to the lack of the most important results; the derivatives of its use and development, and Information of a social, economic, cultural, etc., character.

    Esta actuación consta de un total de 102 viviendas. Se encuentran localizadas en uno de los polígonos del Plan General de Urbanismo del Ayuntamiento de Móstoles (Población del extrarradio de Madrid. Se plantea el estudio y desarrollo, por separado, de tres prototipos diferentes de vivienda con el fin de poder estudiar los resultados experimentales de cada uno: — Viviendas progresivas. — Viviendas con energía solar pasiva. — Viviendas construidas a base de un sistema especial en serie. Los tres plantean el ahorro económico como fin común, pero desde distintas bases de partida: la inversión inicial; el ahorro energético, y el sistema constructivo. El conjunto se encuentra a punto de ser entregado a sus usuarios. Lamentamos lo incompleto de la información debido a la falta de sus resultados más importantes: los derivados de su uso y desarrollo; sociales, económicos, culturales, etcétera.

  10. Modelos experimentales para la evaluación de la acción cicatrizante de medicamentos

    Directory of Open Access Journals (Sweden)

    Raimara González Escobar


    Full Text Available Los intentos del organismo para reparar las lesiones inducidas por agresiones locales comienzan muy precozmente en el proceso de la inflamación, y finalmente concluyen con la reparación y sustitución de las células lesionadas por células sanas. El proceso de cicatrización de una herida en la piel involucra la compleja interacción de muchos tipos de células y ocurre como una cascada secuencial de procesos solapados e íntimamente relacionados. Existen varios modelos farmacológicos experimentales que permiten evaluar la acción cicatrizante de un principio activo, profundizando en los eventos específicos de la cicatrización. En este trabajo se presentarán los detalles de algunos de estos modelos, los cuales son: modelo de lesión inducida por quemadura en curieles, modelo de 6 heridas asépticas en cerdos, promoción de cicatrización por segunda intención en ratas y modelo para predecir la distribución de un medicamento aplicado tópicamente en heridas. Estos ensayos permiten el estudio de múltiples elementos histológicos, bioquímicos, celulares y clínicos, característicos del proceso de cicatrización.The attempts of the organism to repair injuries induced by local aggressions begin very early in the inflammation process and conclude finally with the reparation and substitution of those cells damaged by sound cells. The healing process of a skin wound involves the complex interaction of many types of cells and a sequence of sneaky and closely related processes take place. There are various pharmacological experimental models allowing to evaluate the healing action of an active principle and to go deep into the specific healing events. Details are given in this paper about the following models: model of injury induced by burn in guinea pigs, model of 6 aseptic wounds in pigs, promotion of healing by second intention in rats and a model to predict the distribution of a drug topically applied in wounds. These assays make possible

  11. Proceso de gasificación de biomasa: una revisión de estudios teórico- experimentales

    Directory of Open Access Journals (Sweden)

    Juan Pérez


    Full Text Available La situación energética y medioambiental mundial hace que la generación de energía mediante sistemas basados en energías renovables tome cada vez una mayor relevancia. Estos sistemas tienen una doble ventaja ya que posibilitan la diversificación energética y reducen la dependencia del petróleo a la vez que se disminuyen las emisiones globales de CO2. La biomasa es uno de los recursos energéticos con mayor potencial tanto en países desarrollados como emergentes pero sigue sin sufrir el desarrollo esperado. La gasificación de biomasa es uno de los sistemas con gran potencial ya que permite la generación tanto de energía térmica como eléctrica, mediante tecnologías de bajo costo como los gasificadores de lecho fijo (potencias medias- bajas, o reactores de lecho fluidizado con tecnología más exigente y con mayor capacidad de generación. La transformación de la biomasa en un combustible gaseoso, aprovechable en motores de combustión interna alternativos o en turbinas de gas como combustible, exige que la biomasa atraviese varios procesos termoquímicos, compuestos por reacciones endotérmicas (secado, devolatilización y reducción y exotérmicas (oxidación de volátiles y carbón. La selección de un gasificador requiere conocer las propiedades de la biomasa, mientras que el diseño del mismo exige conocer el fenómeno termoquímico por completo. El objetivo de este trabajo es presentar una revisión actualizada de los diferentes planteamientos tanto teóricos como experimentales desarrollados para estudiar el proceso de gasificación de biomasa, tanto en lecho fijo como en lecho fluidizado, con miras a diseñar gasificadores de biomasa.

  12. Injection and temporary capture of a charged particle beam in an open magnetic configuration. Optimization of the configuration. Case of cylindrical symmetry: A mirror machine; Etude de l'injection et de la capture temporaire d'un faisceau de particules chargees dans une configuration magnetique ouverte. Optimisation de la configuration. Cas de la symetrie de revolution: Machine a miroirs

    Energy Technology Data Exchange (ETDEWEB)

    Capdequi-Peyranere, P. [Commissariat a l' Energie Atomique, Fontenay-aux-Roses (France). Centre d' Etudes Nucleaires


    ordinateur IBM 7094 permettant l'optimisation de la configuration. On donne ensuite les resultats d'une etude numerique statistique de la capture dans la configuration obtenue. Cette etude montre que si l'energie des particules du faisceau est de 1 pour cent superieure a une energie minimum de penetration, la totalite du faisceau peut etre capturee avec une longueur moyenne de capture de 100 metres (50 reflexions entre lesdeux miroirs). Si l'energie est de 4 pour cent superieure a l'energie minimum de penetration la longueur de capture se reduit 40 metres. On etudie la repartition de l'energie de la population des particules capturees en energie perpendiculaire et energie longitudinale au champ magnetique. Les particules injectees etant supposees etre des ions moleculaires d'hydrogene ou des ions lourds CH{sub 4}{sup +}, une etude du temps de capture des protons resultant d'une dissociation des ions injectes sur le gaz neutre est faite. On decrit enfin un modele a electrons destine a apporter une verification experimentale de la capture du faisceau primaire. (auteur)

  13. El AMMI y cluster en la selección de los mejores híbridos experimentales de maíz

    Directory of Open Access Journals (Sweden)

    Guillermo Casta\\u00F1\\u00F3n


    Full Text Available El AMMI y Cluster en la selección de los mejores híbridos experimentales de maiz. En un experimento de maíz repetido en cuatro ambientes, se aplicó el análisis de efectos principales aditivos e interacción multiplicativa (AMMI y el de taxonomía numérica o cluster, para estudiar si la estabilidad del rendimiento de grano (RG de los híbridos ensayados se asociaba con el agrupamiento de los híbridos hecho por el cluster. Las variables que tuvieron más peso en el agrupamiento fueron el RG, altura de la planta (AP,altura de mazorca ( AM y mazorca por planta (PRO, principalmente. De lo encontrado en este trabajo se puede establecer que el AMMI es efectivo en la estimación de la estabilidad de los híbridos experimentales evaluados y que junto con el cluster, pueden ser dos herramientas útiles para hacer una buena selección de material experimental con mayores posibilidades de éxito

  14. Experimental techniques; Techniques experimentales

    Energy Technology Data Exchange (ETDEWEB)

    Roussel-Chomaz, P. [GANIL CNRS/IN2P3, CEA/DSM, 14 - Caen (France)


    This lecture presents the experimental techniques, developed in the last 10 or 15 years, in order to perform a new class of experiments with exotic nuclei, where the reactions induced by these nuclei allow to get information on their structure. A brief review of the secondary beams production methods will be given, with some examples of facilities in operation or under project. The important developments performed recently on cryogenic targets will be presented. The different detection systems will be reviewed, both the beam detectors before the targets, and the many kind of detectors necessary to detect all outgoing particles after the reaction: magnetic spectrometer for the heavy fragment, detection systems for the target recoil nucleus, {gamma} detectors. Finally, several typical examples of experiments will be detailed, in order to illustrate the use of each detector either alone, or in coincidence with others. (author)


    Directory of Open Access Journals (Sweden)

    Yucan EKE


    Full Text Available This research is done for attesting the compliance of the etudes in German composer Ernesto Köhler’s op:33 no I-II-III books that are used in high schools of fine arts, with ability to play the flute concertos in high schools of fine arts 12 grade program, in terms of the knowledge – skills and musical functions they cover and also for contributing to learning by matching the etudes and works which are relevant. Etudes and the chosen concertos are analyzed and separated into templates in terms of melody, rhythm and tone for this research. Opinions are taken from the flute lesson instructors of various universities of Turkey to define the melody, technique and rhythm patterns. As a result of the analysis, Köhler op: 33, Book I, Etudes: 2,3,6,7,8,9,10,11,12; Köhler op: 33, Book II, Etudes:1,3,4,5,8,9,10,11; Köhler op: 33, Book III, Etudes: 1, 2, 4, 5, 6, 8; It is determined that the etudes given above in Köhler books are qualified enough to be preparative and instructive for the ability of playing the flute concertos. Some suggestions are given about which etude(s is instructive for which concerto and what kind of benefits could be gained, by this study. Based on the observed results, various suggestions are made about defining and studying the etudes which meet the technical, rhythmic and melodic features of the target concerto.

  16. Etude sur les tendons en materiaux composites et leur application aux ancrages postcontraints (United States)

    Chennouf, Adil

    L'objectif general de la presente these est d'evaluer le comportement a l'arrachement et au fluage d'ancrages injectes constitues de tendons en materiaux composites afin d'etablir des recommandations plus appropriees et realistes pour le dimensionnement et la conception. Quatre types de tendons en materiaux composites, deux a base de fibres d'aramide et deux a base de fibres de carbone, ont ete utilises dans l'etude. Les travaux de recherche de cette these ont porte notamment sur: (I) Une caracterisation physique et mecanique des tendons en materiaux composites utilises dans l'etude. (II) Une etude en laboratoire sur les coulis de scellement. La premiere etape de cette etude a concerne le developpement d'un coulis de scellement performant adapte aux tendons en materiaux composites et a differentes situations d'injection. La seconde etape a traite des essais de caracterisations physique et mecanique du coulis de scellement developpe comparativement a trois coulis de scellement usuels d'un meme rapport E/L de 0,4. (III) Une etude sur des modeles reduits d'ancrages injectes. (IV) Une etude sur des modeles d'ancrages a grande echelle. La synthese de ces etudes a permis d'enoncer les principales conclusions suivantes: (1) Les valeurs moyennes des charges de rupture des tendons en materiaux composites ont ete de 1% a 29% superieures a celles specifiees par les manufacturiers. (2) L'etude sur les coulis de scellement a permis le developpement de coulis de ciment repondant aux criteres fixes, soient une grande stabilite, une bonne fluidite, une legere expansion et de bonnes caracteristiques mecaniques. (3) Les tendons en materiaux composites ont montre des contraintes d'adherence maximum superieures a celles des tendons en acier. (4) Le type de fibre, la configuration et le fini de surface des tendons en materiaux composites gouvernent leur resistance a l'adherence. (5) L'introduction de sable et d'autres ajouts comme les fines de silice et la poudre d'aluminium au coulis

  17. Some experiments on the high-low transition of quartz; Recherches experimentales sur une transformation du quartz

    Energy Technology Data Exchange (ETDEWEB)

    Mayer, G. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    , nous avons rassemble un grand nombre de resultats numeriques relatifs aux proprietes elastiques, piezoelectriques et optiques du quartz. Certains ont ete collectes dans la litterature; d'autres ont ete obtenus par nous a l'aide de methodes experimentales que nous decrivons, de facon a pouvoir presenter un tableau complet de l'evolution de ces constantes dans un domaine de temperature entourant largement le point de transformation {alpha} - {beta} situe a 574 deg. C. De nouveaux phenomenes ont ete observes au cours de ces mesures. Dans la troisieme partie, nous montrons qu'une meme fonction de la temperature decrit l'evolution de la constante piezoelectrique et de la constante elastique qui s'annulent en phase {beta}. Une seule autre fonction suffit a decrire correctement l'evolution de toutes les autres constantes etudiees, depuis leur valeur en phase {alpha} jusqu'a leur valeur en phase {beta}. A l'aide d'hypotheses sur la nature de l'entropie de transformation, nous essayons enfin d'expliquer la forme de ces fonctions. (auteur)

  18. Etude écologique du Parc National de Mozogo-Gokoro (Cameroun ...

    African Journals Online (AJOL)

    Etude écologique du Parc National de Mozogo-Gokoro (Cameroun): prospections préliminaires de la flore ligneuse et du sol pour sa conservation et son aménagement. ... d'aménagement. Mots clés : Pré-inventaire, diversité compositionnelle, structure, écosystème de référence, menaces anthropiques, aire protégée ...

  19. Etude des boues résiduaires de la station de traitement des eaux de ...

    African Journals Online (AJOL)

    Etude des boues résiduaires de la station de traitement des eaux de Ziga et examen de leur possible valorisation pour la confection de barrières étanches. ... pour caractériser la cohérence et la capacité de faire couler la matière dans les procédés industriels et sur les mesures de filtration de la perméabilité du gâteau

  20. Etude de conception d'un simulateur de commande numérique en ...

    African Journals Online (AJOL)

    or Register. DOWNLOAD FULL TEXT Open Access DOWNLOAD FULL TEXT Subscription or Fee Access. Etude de conception d'un simulateur de commande numérique en tournage. K. A. Kasségné, AS Ajavon, C Carton, A Pouyo. Abstract. (J. de la Recherche Scientifique de l'Université de Lomé, 2000, 4(2): 169-180) ...

  1. The study by means of a photomultiplier of the scintillations produced by {alpha} particles striking a zinc sulphide screen; Etude, au photomultiplicateur, des scintillations produites par les particules {alpha} dans un ecran de sulfure de zinc. Application a la numeration precise des particules {alpha}

    Energy Technology Data Exchange (ETDEWEB)

    Anthony, J.P. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    The object of the study is the accurate counting of {alpha} particles by p-m. detection of their scintillations upon impact with a zinc sulphide screen. The main advantage of the method is the extreme simplicity of the electronics used: the possibility of obtaining a utilizable pulse from the p-m. (EMI5311) without any amplification, and in linear response, is demonstrated. The scintillation produced by an impact on Zn-S has also been studied experimentally. The decrease of light intensity in relation to time may be interpreted by the exponential relation: I = I{sub 0} exp (-t / {tau}) whereby {tau} = (39 {+-} 0,1) 10{sup -6} s. The relation between scintillation intensity and remaining trajectory after travel through a given air-space has also been determined. Possible suitable applications of this method of {alpha} counting are those where good stability and low background are necessary. Results stated bear on air contamination studies, isotopic composition variation measurement of uranium, bismuth content measurement in alloys by irradiation of specimens in a thermal neutron flux and {alpha} count on the Po formed. (author) [French] Ce travail est consacre a l'etude de la numeration precise des particules {alpha} par detection au photomultiplicateur des scintillations produites par ces particules dans un ecran de sulfure de zinc. Le principal avantage de cette methode reside dans l'extreme simplicite de l'appareillage electronique; il est en effet montre qu'il est possible, tout en convoyant une reponse lineaire, d'obtenir du photomultiplicateur (EMI5311) un signal electrique utilisable sans aucune amplification. La scintillation produite par l'impact des particules {alpha} sur un ecran de Zn-S est etudiee experimentalement. La decroissance de l'intensite lumineuse en fonction du temps est interpretable par la relation exponentielle I = I{sub 0} exp (-t / {tau}) avec {tau} = (39 {+-} 0,1) 10{sup -6} s. La relation entre l

  2. Avances en investigación de la discapacidad intelectual de origen genético: tratamientos experimentales en el síndrome X frágil

    Directory of Open Access Journals (Sweden)

    Yolanda de Diego Otero


    Full Text Available De Diego Otero, Y. et al. (2017: “Avances en investigación de la discapacidad intelectual de origen genético: tratamientos experimentales en el síndrome X frágil”. Revista Española de Discapacidad, 5 (I: 217-227.

  3. Singing as a Therapeutic Agent, inThe Etude, 1891-1949. (United States)



    The Etude music magazine, founded by Theodore Presser, was one of a number of popular music magazines published in the years prior to the establishment of the music therapy profession in 1950. During its publication run from 1883 to 1957, over 100 music therapy related articles appeared, including 13 on the health benefits of singing published between 1891 and 1949. Written by authors with diverse backgrounds, such as the famous Battle Creek, Michigan physician John Harvey Kellogg and Boston music critic Louis C. Elson, the articles contained consistent and adamant support regarding the health benefits of singing. The advantages described were both physical and psychological, and were recommended prophylactically for well persons and therapeutically for ill persons. Although the articles varied in perspective, from philosophical to theoretical to pedagogical, there is a consistent holistic medicine theme that appeared almost ahead of its time and no doubt linked to the push for vocal music education in that era. The importance of The Etude in promulgating ideas that helped shape the early practice of music therapy should not be underestimated. For much of its publication run The Etude was the largest music periodical in print, reaching its peak circulation of 250,000 copies per month in 1924.

  4. Experimental study of the test module of the electromagnetic end-cap calorimeter for the ATLAS experiment. Study of the spin correlation in the production of pairs tt-bar; Etude experimentale des performances du module 0 du calorimetre electromagnetique bouchon d'ATLAS. Etude de la correlation de spin dans la production des paires tt-bar au LHC

    Energy Technology Data Exchange (ETDEWEB)

    Hinz, L


    LHC, the future CERN proton collider, will start in 2006. It will be devoted to a better understanding of the Standard Model and new physics research. With a 10 {integral}b{sup -1} per year at low luminosity during the first three years, then 100 {integral}b{sup -1} per year, and energy of 14 TeV in the center of mass, the LHC is designed to discover the Standard or SUSY Higgs boson, or probe signature of new physics. ATLAS, one of the four experiments at the LHC, can study a large physics range, as Higgs boson, top and bottom, gauge bosons and new particles expected by SUSY model or other models beyond the Standard Model. The CPPM laboratory is responsible of a part of the electromagnetic end-cap calorimeter for the ATLAS experiment. In 1999, an ATLAS-like prototype of module was stacked in Marseille and intensively tested at CERN. Description of the calorimeter and a part of test-beam results are presented in this PhD manuscript. In parallel, a study about potentiality of the tt-bar spin correlation measurement was done. The high tt-bar statistic produced at the LHC allows to explore the quark top properties in details and being sensitive to new physics phenomena. Signatures of such physics can be extracted from tt-bar decay product angular distributions which are sensitive to tt-bar spin correlation. (authors)

  5. Prevention of bacterial adhesion

    DEFF Research Database (Denmark)

    Klemm, Per; Vejborg, Rebecca Munk; Hancock, Viktoria


    Management of bacterial infections is becoming increasingly difficult due to the emergence and increasing prevalence of bacterial pathogens that are resistant to available antibiotics. Conventional antibiotics generally kill bacteria by interfering with vital cellular functions, an approach...... that imposes selection pressure for resistant bacteria. New approaches are urgently needed. Targeting bacterial virulence functions directly is an attractive alternative. An obvious target is bacterial adhesion. Bacterial adhesion to surfaces is the first step in colonization, invasion, and biofilm formation....... As such, adhesion represents the Achilles heel of crucial pathogenic functions. It follows that interference with adhesion can reduce bacterial virulence. Here, we illustrate this important topic with examples of techniques being developed that can inhibit bacterial adhesion. Some of these will become...

  6. Study of current instabilities in high resistivity gallium arsenide; Etude des instabilites de courant dans l'arseniure de gallium de haute resistivite

    Energy Technology Data Exchange (ETDEWEB)

    Barraud, A. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    'arseniure de gallium de haute resistivite. Nous montrons que ces oscillations sont associees a la progression lente d'un domaine de fort champ electrique a travers tout l'echantillon. L'etude experimentale des proprietes de ces instabilites nous a permis de differencier ce phenomene de l'effet Gunn, des effets acoustoelectriques et des effets de contacts. Nous proposons pour rendre compte de ce type d'instabilites un mecanisme de piegeage differentiel sur des impuretes repulsives, capable de depeupler la bande de conduction a champ electrique eleve et de donner naissance a un domaine de champ intense. Le developpement qualitatif de ce modele nous permet de rendre compte de toutes les proprietes des cristaux d'arseniure de gallium de haute resistivite soumis a un champ electrique eleve: augmentation de la constante de Hall, existence d'un seuil de tension pour ces oscillations, formation de domaines de champ intense, lenteur de la propagation de ces domaines, enfin possibilite de renverser le sens de la propagation du domaine sans detruire celui-ci. Le developpement quantitatif du modele permet de calculer les differentes grandeurs caracteristiques de ces instabilites. La comparaison avec l'experience montre une bonne concordance, les legeres divergences provenant surtout de la meconnaissance actuelle des proprietes de transport dans l'arseniure de gallium aux champs electriques eleves. A la lumiere de ce modele, il apparait que le phenomene d'instabilite etudie peut se produire pour une gamme etendue de concentrations en centres repulsifs, et pour toute une gamme de resistivites. Ceci explique qu'il apparaisse systematiquement dans l'arseniure de gallium de moyenne et haute resistivite. (auteurs)

  7. Etude de la diversité, de l'endémisme et de la distribution spatiale ...

    African Journals Online (AJOL)

    Etude de la diversité, de l'endémisme et de la distribution spatiale des Rubiaceae de Côte d'Ivoire. Fatou Nanou Tuo, Kouao Jean Koffi, Akossoua Faustine Kouassi, Moussa Kone, Bakayoko Adama, Jan Bogaert ...

  8. Etude de la qualité organoleptique de trois variétés de mangues ...

    African Journals Online (AJOL)

    Etude de la qualité organoleptique de trois variétés de mangues Amélie , Lippens , Brooks séchées au cours du stockage par technique de brunissement enzymatique des peroxydases (POD) et des polyphénoloxydases (PPO)

  9. Etude de l'impact de la variation de la température sur les activités ...

    African Journals Online (AJOL)

    Etude de l'impact de la variation de la température sur les activités microbiennes : application à une population de Listeria monocytogenes CIP 7831, Listeria monocytogenes Scott A CIP 103575 et Escherichia coli ATCC 25922.

  10. Bio-metric study of pig karyotype; Etude biometrique du caryotype du porc

    Energy Technology Data Exchange (ETDEWEB)

    Haag, J.; Lacourly, N.; Nizza, P. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    This study has a twofold purpose, the former is to determine the swine karyotype as accurately as possible, the latter is to try and develop a method of automatic classification and to show its possibilities and limits. (authors) [French] Cette etude a un double objet: d'une part, de definir de la facon aussi precise que possible le caryotype du porc et d'autre part, de tenter une methode de classification automatique et d'en montrer les possibilites ainsi que les limites. (auteurs)

  11. Studying bacterial multispecies biofilms

    DEFF Research Database (Denmark)

    Røder, Henriette Lyng; Sørensen, Søren Johannes; Burmølle, Mette


    , but the identity and significance of interspecies bacterial interactions is neglected in these analyses. There is therefore an urgent need for bridging the gap between metagenomic analysis and in vitro models suitable for studies of bacterial interactions.Bacterial interactions and coadaptation are important......The high prevalence and significance of multispecies biofilms have now been demonstrated in various bacterial habitats with medical, industrial, and ecological relevance. It is highly evident that several species of bacteria coexist and interact in biofilms, which highlights the need for evaluating...

  12. Evaluación de 3 dietas experimentales como sustitutos parciales de alimento fresco para la alimentación reproductores silvestres y reproductores de piscina en la especie penaeus vannamei


    Mendoza, Stalin


    Evaluación de 3 dietas experimentales como sustitutos parciales de alimento fresco para la alimentación reproductores silvestres y reproductores de piscina en la especie Penaeus vannamei La mayoría de los laboratorios del Ecuador dependen de nauplios y postlarvas de maduración porque en nuestro medio la disponibilidad de la larva silvestre no es constante, debido a causas climatológicas.

  13. Análisis conductual aplicado en neuropsicología: fundamentos teóricos, experimentales y empíricos.

    Directory of Open Access Journals (Sweden)

    José Alonso Aguilar-Valera


    Full Text Available El presente artículo presenta una revisión teórica donde se expone la importancia que tienen los principios y técnicas conductuales dentro del ámbito de la rehabilitación neuropsicológica. En la intervención clínica con pacientes víctimas de disfunción, daño o deterioro neurológico, donde las consecuencias directas sobre el comportamiento son significativas, el uso de las estrategias provenientes de las técnicas de modificación de conducta (conductuales y/o conductual-cognitivas son importantes para el tratamiento de los diversos síndromes neuroconductuales, estando dentro de las competencias del especialista en esta área aplicada de las neurociencias. Además de describirse tanto aspectos teóricos como experimentales de principal relevancia para estos fines, también se mencionarán algunos estudios empíricos llevados a cabo, bajo una metodología de caso único, para una mejor comprensión sobre la utilidad aplicativa de los principios conductuales en el ámbito de la neuropsicología clínica.

  14. Evaluación del desempeño de métodos experimentales de identificación a lazo abierto

    Directory of Open Access Journals (Sweden)



    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} En este trabajo se presenta una selección de los métodos experimentales para la identificación de sistemas a lazo abierto que con mayor frecuencia se enuncian en la literatura. Se lleva a cabo la comparación de dichas técnicas sobre la base de la sumatoria del error cuadrático cometido en simulación. Se brinda además un análisis sobre el comportamiento de cada uno de los procedimientos, recomendando que métodos utilizar en dependencia al modelo requerido en la estimación.

  15. Aplicación práctica de métodos estereológicos renales en modelos animales experimentales

    Directory of Open Access Journals (Sweden)

    María Teresa Fernández García


    Full Text Available Los riñones son órganos vitales que realizan funciones de excreción, equilibrio hidroelectrolítico y producción de hormonas. La nefrona es su unidad estructural y funcional. El número, tamaño y distribución de los componentes de la nefrona contienen información relevante sobre la función renal. La estereología es una rama de la morfometría que permite, aplicando reglas matemáticas, obtener información tridimensional de estructuras biológicas a partir de cortes microscópicos bidimensionales, seriados, paralelos y equidistantes. Ante la complejidad de los estudios estereológicos y la carencia de una bibliografía clara sobre el desarrollo de los mismos, el objetivo de este trabajo es explicar de forma sencilla y con ejemplos, utilizando un modelo animal, los conceptos básicos de estereología, así como el cálculo de los principales parámetros estereológicos renales y que estos puedan ser aplicados en futuros estudios experimentales.

  16. Nuevos datos experimentales sobre parámetros hidrogeológicos de la Cuenca de Madrid obtenidos a partir de muestras de sondeos

    Directory of Open Access Journals (Sweden)

    Villarroya Gil, F. I.


    Full Text Available A critical review of main hydrogeological parameters (porosity, permeability and specific storage coefficient of Madrid Basin Detrital Aquifer is done. For this study, data from the bibliography and new laboratory analysis of grain size distribution (26, dry density (26, specific weight (26, permeability (20 and consolidation test (6 samples from three boreholes are used. The results are quite similar to those than already exist in the literature, although the porosity obtained is higher.Se hace una revisión crítica de los principales parámetros hidrogeológicos (porosidad, permeabilidad y coeficiente de almacenamiento específico, del Terciario detrítico de la cuenca de Madrid. Para este estudio además de los datos existentes en la bibliografía, se han realizado una serie de análisis de laboratorio desarrollados en las instalaciones del SGOP obteniendo, de esta manera, nuevos valores experimentales de los citados parámetros, mediante ensayos granulométricos (26, de densidad seca (26, de peso específico (26, de permeabilidad (20 y ensayos edométricos (6. Las muestras proceden de tres sondeos realizados en el casco urbano de Madrid por el SGOP (MOPU. Los resultados obtenidos coinciden, en gran medida, con los existentes en la bibliografía, si bien, los valores de porosidad total son marcadamente más altos.

  17. Contacto normal entre un medio elasto-plástico continuo y una esfera rígida : observaciones experimentales y computacionales

    Directory of Open Access Journals (Sweden)

    Ángela María Pedraza


    Full Text Available Se presenta en este trabajo una investigación experimental y numérica del contacto normal de una esfera rígida con una placa plana de material cuyo comportamiento mecánico se asume de tipo elasto-plástico. Los esfuerzos locales bajo el punto de contacto se han obtenido experimentalmente mediante visualización foto-elástica. Se ha evaluado numéricamente la evolución de los bulbos de esfuerzo bajo el punto contacto como función del tiempo y se ha efectuado la comparación con las observaciones experimentales. Los resultados indican que un modelo elasto-plástico tridimensional reproduce de manera apropiada las características principales observadas en los experimentos físicos. Este ejercicio puramente académico y de carácter didáctico enfatiza en el enorme potencial de los elementos finitos en la solución de problemas de deformación bajo diferentes tipos de modelos constitutivos y pone de manifiesto la enorme utilidad del modelamiento matemático y computacional basado en aproximaciones continuas de la materia

  18. Características experimentales y neurobiología de la inhibición latente en el paradigma de aprendizaje aversivo gustativo

    Directory of Open Access Journals (Sweden)

    Andrés Molero Chamizo


    Full Text Available El procedimiento conductual para inducir aprendizaje aversivo gustativo es susceptible de diversas modificaciones que implican procesos complejos de aprendizaje. La inhibición latente de la respuesta aprendida es uno de estos procesos ampliamente descritos en el paradigma de aversión gustativa. El objetivo de esta revisión es mostrar las características experimentales de la inhibición latente en aprendizaje aversivo gustativo y describir los mecanismos neurobiológicos conocidos de este fenómeno. En conjunto los datos sugieren que las peculiares características del condicionamiento aversivo gustativo y de sus sustratos neurales pueden requerir procesos y estructuras nerviosas diferenciales para la inhibición latente cuando se trata de este aprendizaje. Conclusiones: Los mecanismos cerebrales de algunos fenómenos complejos de aprendizaje (como por ejemplo la inhibición latente pueden variar, e incluso ser independientes, en función del procedimiento empleado.

  19. The formation of scientists and technicians at the 'Centre d'Etudes Nucleaires' at Saclay

    International Nuclear Information System (INIS)

    Debiesse, J.


    The considerable needs in research workers and scientists which are asked by the nuclear energy obliged the Commissariat a l'Energie atomique to deal with a particular effort to increase the quantitative and qualitative formation of scientists. Most various ways have been used. 1- A National Institute of Nuclear Sciences and Nuclear Techniques was created, by a joint decree of the Prime Minister and the Minister for National Education (june 18, 1957). This Institute of Higher Teaching (250 students) indulges in the following matters: atomic engineering, quantum mechanics, theory and technic of particle accelerators, special metallurgy, radiobiology, thermic and mechanics of fluids. 2- An associated centre of the 'Conservatoire National des Arts et Metiers' was created (200 students) for technical assistants, drawers, etc. 3- In contribution with both electronic industry and Ministry of Work, the Centre d'Etudes Nucleaires contributes to an accelerated formation of technical assistants into Professional Centres. Conclusion: Training of scientists and research workers is one of the most important activities of the Centre d'Etudes Nucleaires de Saclay. Without losing its technical efficiency, it has supplied and varied means adapted to the various purposes that we shall reach. (author) [fr

  20. [Spontaneous bacterial peritonitis]. (United States)

    Strauss, Edna; Caly, Wanda Regina


    Spontaneous bacterial peritonitis occurs in 30% of patients with ascites due to cirrhosis leading to high morbidity and mortality rates. The pathogenesis of spontaneous bacterial peritonitis is related to altered host defenses observed in end-stage liver disease, overgrowth of microorganisms, and bacterial translocation from the intestinal lumen to mesenteric lymph nodes. Clinical manifestations vary from severe to slight or absent, demanding analysis of the ascitic fluid. The diagnosis is confirmed by a number of neutrophils over 250/mm3 associated or not to bacterial growth in culture of an ascites sample. Enterobacteriae prevail and Escherichia coli has been the most frequent bacterium reported. Mortality rates decreased markedly in the last two decades due to early diagnosis and prompt antibiotic treatment. Third generation intravenous cephalosporins are effective in 70% to 95% of the cases. Recurrence of spontaneous bacterial peritonitis is common and can be prevented by the continuous use of oral norfloxacin. The development of bacterial resistance demands the search for new options in the prophylaxis of spontaneous bacterial peritonitis; probiotics are a promising new approach, but deserve further evaluation. Short-term antibiotic prophylaxis is recommended for patients with cirrhosis and ascites shortly after an acute episode of gastrointestinal bleeding.

  1. Interfering with bacterial gossip

    DEFF Research Database (Denmark)

    Bjarnsholt, Thomas; Tolker-Nielsen, Tim; Givskov, Michael


    defense. Antibiotics exhibit a rather limited effect on biofilms. Furthermore, antibiotics have an ‘inherent obsolescence’ because they select for development of resistance. Bacterial infections with origin in bacterial biofilms have become a serious threat in developed countries. Pseudomonas aeruginosa...... that appropriately target bacteria in their relevant habitat with the aim of mitigating their destructive impact on patients. In this review we describe molecular mechanisms involved in “bacterial gossip” (more scientifically referred to as quorum sensing (QS) and c-di-GMP signaling), virulence, biofilm formation......, resistance and QS inhibition as future antimicrobial targets, in particular those that would work to minimize selection pressures for the development of resistant bacteria....

  2. Bacterial surface adaptation (United States)

    Utada, Andrew


    Biofilms are structured multi-cellular communities that are fundamental to the biology and ecology of bacteria. Parasitic bacterial biofilms can cause lethal infections and biofouling, but commensal bacterial biofilms, such as those found in the gut, can break down otherwise indigestible plant polysaccharides and allow us to enjoy vegetables. The first step in biofilm formation, adaptation to life on a surface, requires a working knowledge of low Reynolds number fluid physics, and the coordination of biochemical signaling, polysaccharide production, and molecular motility motors. These crucial early stages of biofilm formation are at present poorly understood. By adapting methods from soft matter physics, we dissect bacterial social behavior at the single cell level for several prototypical bacterial species, including Pseudomonas aeruginosa and Vibrio cholerae.

  3. Bacterial Meningitis in Infants

    Directory of Open Access Journals (Sweden)

    J Gordon Millichap


    Full Text Available A retrospective study of 80 infantile patients (ages 30-365 days; 47 male, 33 female with culture-proven bacterial meningitis seen over a 16 year period (1986-2001 is reported from Taiwan.

  4. Factitious Bacterial Meningitis Revisited (United States)

    Peterson, E.; Thrupp, L.; Uchiyama, N.; Hawkins, B.; Wolvin, B.; Greene, G.


    Nonviable gram-negative bacilli were seen in smears of cerebrospinal fluid from eight infants in whom bacterial meningitis was ruled out. Tubes from commercial kits were the source of the factitious organisms. PMID:7153328

  5. Vimentin in Bacterial Infections

    DEFF Research Database (Denmark)

    Mak, Tim N; Brüggemann, Holger


    filaments (IFs). IFs have not only roles in maintaining the structural integrity of the cell, but they are also involved in many cellular processes including cell adhesion, immune signaling, and autophagy, processes that are important in the context of bacterial infections. Here, we summarize the knowledge...... about the role of IFs in bacterial infections, focusing on the type III IF protein vimentin. Recent studies have revealed the involvement of vimentin in host cell defenses, acting as ligand for several pattern recognition receptors of the innate immune system. Two main aspects of bacteria......-vimentin interactions are presented in this review: the role of vimentin in pathogen-binding on the cell surface and subsequent bacterial invasion and the interaction of cytosolic vimentin and intracellular pathogens with regards to innate immune signaling. Mechanistic insight is presented involving distinct bacterial...

  6. Loxoscelismo en Chile: estudios epidemiológicos, clínicos y experimentales Loxoscelism in Chile: epidemiological, clinical and experimental studies

    Directory of Open Access Journals (Sweden)

    Hugo Schenone


    Full Text Available Se presenta un enfoque panorámico de estudios epidemiológicos, clínicos y experimentales referentes a Loxosceles laeta y loxoscelismo efectuados en 1955-1988 en Santiago, Chile. Se estudiaron 216 casos de loxoscelismo. Los hechos más relevantes fueron: 52,8% correspondió a mujeres; edad entre 7 meses y 78 años; 84,3% fué loxoscelismo cutáneo (LO y 15,7% loxoscelismo cutáneo-visceral (LCV; 73,6% sucedió en época calurosa; en 86,6% el accidente ocurrió en la vivienda, especialmente en dormitorios, mientras la persona dormía o se vestía. La araña fué vista en 60,2% de los casos e identificada en laboratorio como L. laeta en 17,7% (10,6% de los 216 casos. Los sitios más frecuen temente afectados fueron las extremidades con 67,6%, lancetazo urente fué el síntoma inicial más frecuente. Dolor, edema y placa livedoide, la cual posteriormente se transformaría en escara necrótica, fueron las manifestaciones locales predominantes. En LCV hematuria y hemoglobinuria fueron constantes, ictericia, fiebre y compromiso de conciencia se presentaron en la mayoría de los casos. Tratamiento: LC con antihistamínicos o corticoides inyectables, LCV con corti-coides inyectables. La condición de los pacientes en el último control fué: curación completa en 75,5%, curación con secuela cicatrizal en 8,3%, muerte en 3,7% (todos con LCV y abandono en 12,5%. Adicionalmente, se ha efectuado una serie de estudios experimentales, tanto in vivo como in vitro para esclarecer aspectos básicos sobre el veneno de L. laeta y el tratamiento del loxoscelismo.A panoramic sight of epidemiological, clinical and experimental studies, referring to Loxosceles laeta and loxoscelism, carried out in 1955-1988, in Santiago, Chile is presented. Two-hundred and sixteen cases of loxosce lism were studied. The most relevant features were: 84.3% corresponded to cutaneous loxosce lism (CD and 15.7% to viscerocutaneous loxos celism (VCD; 73.6% ocurred in hot season; in 86

  7. [Diagnosis of bacterial vaginosis]. (United States)

    Djukić, Slobodanka; Ćirković, Ivana; Arsić, Biljana; Garalejić, Eliana


    Bacterial vaginosis is a common, complex clinical syndrome characterized by alterations in the normal vaginal flora. When symptomatic, it is associated with a malodorous vaginal discharge and on occasion vaginal burning or itching. Under normal conditions, lactobacilli constitute 95% of the bacteria in the vagina. Bacterial vaginosis is associated with severe reduction or absence of the normal H2O2-producing lactobacilli and overgrowth of anaerobic bacteria and Gardnerella vaginalis, Atopobium vaginae, Mycoplasma hominis and Mobiluncus species. Most types of infectious disease are diagnosed by culture, by isolating an antigen or RNA/DNA from the microbe, or by serodiagnosis to determine the presence of antibodies to the microbe. Therefore, demonstration of the presence of an infectious agent is often a necessary criterion for the diagnosis of the disease. This is not the case for bacterial vaginosis, since the ultimate cause of the disease is not yet known. There are a variety of methods for the diagnosis of bacterial vaginosis but no method can at present be regarded as the best. Diagnosing bacterial vaginosis has long been based on the clinical criteria of Amsel, whereby three of four defined criteria must be satisfied. Nugent's scoring system has been further developed and includes validation of the categories of observable bacteria structures. Up-to-date molecular tests are introduced, and better understanding of vaginal microbiome, a clear definition for bacterial vaginosis, and short-term and long-term fluctuations in vaginal microflora will help to better define molecular tests within the broader clinical context.


    Directory of Open Access Journals (Sweden)

    Martín A. Aréchiga P.


    Full Text Available En la naturaleza, los pigmentos tienen la principal función de colorear; sin embargo, también tienen efectos sobre el estado de salud y desarrollo de los organismos. Los compuestos sintéticos dirigidos a incrementar la coloración en organismos con importancia comercial, representan entre el 15 al 20% del costo de la dieta. Por lo anterior, la investigación sobre pigmentos de origen natural (más baratos y menos tóxicos se ha incrementado sustancialmente en los últimos años. Las antocianinas pueden ser utilizadas como una alternativa para la pigmentación de organismos. En este estudio se utilizaron los cálices de Hibiscus sabdariffa, planta popular que acumula antocianinas que proporciona su característico color rojo. Langostinos Macrobrachium tenellum fueron alimentados con dietas que incluían cálices de H. sabdariffa (jamaica bajo los siguientes tratamientos: i pigmento extraído con agua, ii pigmento extraído con etanol acidificado [0.1 M HCl (v/v], y iii harina de cálices. El efecto pigmentante de las dietas experimentales se comparó con langostinos sometidos a una dieta control sin pigmentos añadidos y con langostinos extraídos del medio silvestre. Los langostinos alimentados con la dieta de inclusión de polvo de jamaica mostraron la mayor expresión de eritróforos (color rojo en el exoesqueleto del pleon (abdomen y ganancia en longitud y peso, después de 60 días. Estos resultados sugieren que H. sabdariffa puede ser utilizado como complemento alimenticio natural, puesto que tiene un efecto positivo en los parámetros de crecimiento y pigmentación.

  9. Generalities on the dynamic behaviour of rapid reactors. Preliminary studies on Rapsodie; Generalites sur le comportement dynamique des piles rapides. Etudes preliminaires de rapsodie

    Energy Technology Data Exchange (ETDEWEB)

    Campan, J.L.; Chaumont, J.P.; Clauzon, P.P.; Ghesquiere, G.; Leduc, J.; Schmitt, A.P.; Zaleski, C.P. [Commissariat a l' Energie Atomique, Cadarache (France). Centre d' Etudes Nucleaires


    The study of the dynamic behaviour of fast reactors may be divided into three section: 1. Stability studies around equilibrium power only the linear case was examining. S. Transient studies in the case of usual reactor operation (shut down, scram, etc.) with thermal shocks evaluation, for instance. 3. Explosion studies, for the maximum credible accidents. This report presents the status of the studies performed at the 'Physics Research Department' at Cadarache. Methods used are detailed and illustrated with the results obtained on a preliminary metallic core of the Rapsodie Reactor. (authors) [French] Le comportement dynamique des piles rapides, se presente tout naturellement sous trois aspects: 1. Etude de stabilite autour d'un regime d'equilibre (nous nous sommes bornes ici au cas lineaire). 2. Etude de regimes transitoires lors des operations normales de pile (arret, arret d'urgence, etc.) avec evaluation des chocs thermiques par exemple. 3. Etude des regimes transitoires de caractere explosif lors des accidents les plus graves possibles. Ce rapport presente l'etat des etudes a la date du 20 decembre 1961 a la Section d'Etudes de Piles Rapides a CADARACHE. Les methodes employees ont ete detaillees et illustrees a partir des resultats obtenus sur une premiere version 'combustible metallique' de Rapsodie. (auteurs)

  10. 107 Etude des facteurs de variation des prix d'intérêt des matières ...

    African Journals Online (AJOL)


    Imen BELHADJ SLIMEN et Taha NAJAR. Etude des facteurs de variation des prix d'intérêt des matières premières de substitution utilisées dans les aliments concentrés des animaux d'élevage. Imen BELHADJ SLIMEN1,2* et Taha NAJAR1,2. 1Institut National Agronomique de Tunisie, Département Ressources Animales, ...

  11. Etude de la qualité organoleptique de trois variétés de mangues ...

    African Journals Online (AJOL)

    Etude de la qualité organoleptique de trois variétés de mangues Amélie , Lippens , Brooks séchées au cours du stockage par technique de brunissement enzymatique des peroxydases (POD) et des ... Les activités les plus élevées en polyphénoloxydase et en peroxydase étaient respectivement de 19.75×10-3 ± 0.01 et 52.

  12. Etude cinétique de la dégradation de l'Endosufane en solution ...

    African Journals Online (AJOL)

    Etude cinétique de la dégradation de l'Endosufane en solution. S Traore, K Mamadou, A Dembele, V Yao, Y Bekro, P Mazellier, B Legube, P Houenou. Abstract. Les pesticides sont fréquemment utilisés en Côte d'Ivoire. Leur détection dans les eaux de forage témoigne de leur mauvais usage et d'un grave problème ...

  13. Etude in vitro de l'activité antibactérienne et antifongique de l'extrait ...

    African Journals Online (AJOL)


    UNR –Journal Etudes Rwandaises– Series C:Life Science & Natural Sciences– August 2009. 12. Tableau 4 : Test de l'activité de l'extrait et fractions à différents degrés de pH. Extrait hydroéthanolique. F2. F3. Souches microbiennes. A B C D E. A B C D E A B C D E. pH : 5. - - - - -. - - - - - - - - - -. pH : 7. - - - - - - - - - - - - - - -.

  14. Spectroscopic study of near closed-shell nuclei Z = 50, by means of the ({sup 3}He,d) and (d, {sup 3}He) reactions on all even tin isotopes, and the ({sup 3}He, d) reaction on the A = 113 and A = 115 indium isotopes; Etude spectroscopique des noyaux au voisinage de la couche fermee Z = 50, au moyen des reactions ({sup 3}He, d) et (d, {sup 3}He) sur tous les isotopes pairs de l'etain, et de la reaction ({sup 3}He, d) sur les isotopes 113, 115 de l'indium

    Energy Technology Data Exchange (ETDEWEB)

    Harar, S. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    A systematic study of ({sup 3}He, d) reactions at 18 MeV incident energy and (d, {sup 3}He) reactions at 22 MeV incident energy on {sup 112,114,116,118,120,122,124}Sn is presented. The distorted wave Born approximation (DWBA) is used to extract informations on (a) the transferred angular momentum l{sub p}, (b) the change of parity and (c) the spectroscopic factor. We have investigated the influence of the proton-neutron residual interaction on the spread of the pure proton configuration (particle or hole), and on the observed level sequence. Experimental results and predictions of the Kisslinger and Sorensen model are in reasonable agreement. A study of {sup 113,115}In ({sup 3}He, d) {sup 114,116}Sn reactions shows strong experimental evidence that the proton particle hole components are weak in the first excited states of tin nuclei. Furthermore, highly excited states ({approx} 4 to 6 MeV) having a simple structure with one predominant particle-hole configuration have been located. (authors) [French] Nous presentons une etude systematique des reactions ({sup 3}He, d) a 18 MeV et (d, {sup 3}He) a 22 MeV sur {sup 112,114,116,118,120,122,124}Sn. L'approximation des ondes deformees (DWBA) est utilisee pour obtenir des informations sur le moment angulaire transfere l{sub p}, sur le changement de parite et le facteur spectroscopique. Nous avons etudie l'influence de l'interaction residuelle proton-neutron sur la repartition de l'intensite a une seule particule ou trou de proton, et sur les sequences des niveaux observes. Les resultats experimentaux et les previsions theoriques du modele de Kisslinger et Sorensen sont en assez bon accord. L'etude des reactions {sup 113,115}In ({sup 3}He, d) {sup 114,116}Sn apporte la preuve experimentale que les composantes particule - trou de protons des premiers etats excites des noyaux residuels sont faibles. Elle permet aussi de localiser des niveaux a une energie d'excitation elevee ({approx} 4 a 6 Me

  15. Validez Estructurada para una investigación cuasi-experimental de calidad: se cumplen 50 años de la presentación en sociedad de los diseños cuasi-experimentales

    Directory of Open Access Journals (Sweden)

    Paula Fernández-García


    Full Text Available Investigación cuasi-experimental es aquella que tiene como objetivo poner a prueba una hipótesis causal manipulando (al menos una variable independiente donde por razones logísticas o éticas no se puede asignar las unidades de investigación aleatoriamente a los grupos. Debido a que muchas decisiones a nivel social se toman en base al resultado de investigaciones con estas características, es imperativo que tengan una planificación exquisita de la aplicación del tratamiento, del control en el proceso de la investigación y del análisis de los datos. El pasado año 2013 los diseños cuasi-experimentales cumplieron 50 años, y este trabajo es un homenaje a Campbell y a todos los investigadores que día a día aportan ideas para mejorar el método cuasi-experimental en alguno de sus aspectos. De la mano de una revisión de las investigaciones cuasi-experimentales publicadas en un período de 11 años en tres revistas de Psicología destacamos algunos aspectos que se refieren al cuidado del método. Finalizamos el trabajo proponiendo el concepto de Validez Estructurada, que en resumen, es el hilo conductor que debe seguir la realización de toda investigación para poner a prueba con garantía las hipótesis que responden a los objetivos que en ella se plantean, concretamente, en las investigaciones cuasi-experimentales.

  16. Bacterial Cell Mechanics. (United States)

    Auer, George K; Weibel, Douglas B


    Cellular mechanical properties play an integral role in bacterial survival and adaptation. Historically, the bacterial cell wall and, in particular, the layer of polymeric material called the peptidoglycan were the elements to which cell mechanics could be primarily attributed. Disrupting the biochemical machinery that assembles the peptidoglycan (e.g., using the β-lactam family of antibiotics) alters the structure of this material, leads to mechanical defects, and results in cell lysis. Decades after the discovery of peptidoglycan-synthesizing enzymes, the mechanisms that underlie their positioning and regulation are still not entirely understood. In addition, recent evidence suggests a diverse group of other biochemical elements influence bacterial cell mechanics, may be regulated by new cellular mechanisms, and may be triggered in different environmental contexts to enable cell adaptation and survival. This review summarizes the contributions that different biomolecular components of the cell wall (e.g., lipopolysaccharides, wall and lipoteichoic acids, lipid bilayers, peptidoglycan, and proteins) make to Gram-negative and Gram-positive bacterial cell mechanics. We discuss the contribution of individual proteins and macromolecular complexes in cell mechanics and the tools that make it possible to quantitatively decipher the biochemical machinery that contributes to bacterial cell mechanics. Advances in this area may provide insight into new biology and influence the development of antibacterial chemotherapies.

  17. Competencias científica promovidas en las actividades experimentales y su articulación con los lineamientos curriculares para el área de Ciencias Naturales en la básica primaria


    Ruiz Ortega, F.


    Teniendo en cuenta la importancia de la formación de ciudadanos competentes, dadas las nuevas políticas nacionales e internacionales, la investigación que se plantea tiene como propósito establecer los puntos de encuentro y distanciamiento al comparar la promoción de competencias científicas en prácticas experimentales de un escenario educativo de la ciudad de Barcelona y su coherencia y/o pertinencia con los lineamientos curriculares del área de ciencias naturales para la educación primaria ...

  18. Aproximación al estudio de las estrategias didácticas en ciencias experimentales en formación inicial del profesorado de Educación Secundaria: descripción de una experiencia


    Natalia Jiménez-Tenorio; José María Oliva


    Se fundamenta y describe una actividad orientada a la formación inicial de profesores de secundaria de especialidades de ciencias experimentales. La actividad está planteada como intento de aproximación al estudio de distintas estrategias didácticas, más o menos insertas dentro del marco socio-constructivista: “aprendizaje por descubrimiento”, “cambio conceptual”, “enseñanza por investigación entorno a problemas” y “enfoques CTS”. La actividad incluye una serie de estudios de caso (cuatro en ...

  19. Determinación teórica y comparación con datos experimentales de la cantidad de agua producida por una celda de combustible de membrana de intercambio protónico


    Trejos Valencia, Jorge Mario


    Las celdas de combustible son dispositivos electroquímicos eficientes que además de producir electricidad y calor, producen agua como subproducto durante su operación, pero poco se ha investigado acerca de la producción de agua a partir de una celda de combustible. En este proyecto se diseñó y construyó una celda de combustible de membrana de intercambio protónico (CCMIP) para determinar teóricamente y comparar con datos experimentales la cantidad de agua producida por ésta. Para el desarroll...

  20. Bacterial meningitis in children

    International Nuclear Information System (INIS)

    Marji, S.


    To demonstrate the epidemiology, clinical manifestations and bacteriological profile of bacterial meningitis in children beyond the neonatal period in our hospital. This was a retrospective descriptive study conducted at Prince Rashid Hospital in Irbid, Jordan. The medical records of 50 children with the diagnosis of bacterial meningitis during 4 years period, were reviewed. The main cause of infection was streptococcus pneumoniae, followed by Haemophilus influenza and Niesseria meningitides. Mortality was higher in infants and meningococcal infection, while complications were more encountered in cases of streptococcus pneumoniae. Cerebrospinal fluid culture was positive in 11 cases and Latex agglutination test in 39. There is a significant reduction of the numbers of bacterial meningitis caused by Haemophilus influenza type B species. (author)

  1. Interfering with bacterial gossip

    DEFF Research Database (Denmark)

    Bjarnsholt, Thomas; Tolker-Nielsen, Tim; Givskov, Michael


    Biofilm resilience poses major challenges to the development of novel antimicrobial agents. Biofilm bacteria can be considered small groups of “Special Forces” capable of infiltrating the host and destroying important components of the cellular defense system with the aim of crippling the host...... defense. Antibiotics exhibit a rather limited effect on biofilms. Furthermore, antibiotics have an ‘inherent obsolescence’ because they select for development of resistance. Bacterial infections with origin in bacterial biofilms have become a serious threat in developed countries. Pseudomonas aeruginosa...... that appropriately target bacteria in their relevant habitat with the aim of mitigating their destructive impact on patients. In this review we describe molecular mechanisms involved in “bacterial gossip” (more scientifically referred to as quorum sensing (QS) and c-di-GMP signaling), virulence, biofilm formation...

  2. Diagnosis of bacterial vaginosis

    Directory of Open Access Journals (Sweden)

    Đukić Slobodanka


    Full Text Available Bacterial vaginosis is a common, complex clinical syndrome characterized by alterations in the normal vaginal flora. When symptomatic, it is associated with a malodorous vaginal discharge and on occasion vaginal burning or itching. Under normal conditions, lactobacilli constitute 95% of the bacteria in the vagina. Bacterial vaginosis is associated with severe reduction or absence of the normal H2O2­producing lactobacilli and overgrowth of anaerobic bacteria and Gardnerella vaginalis, Atopobium vaginae, Mycoplasma hominis and Mobiluncus species. Most types of infectious disease are diagnosed by culture, by isolating an antigen or RNA/DNA from the microbe, or by serodiagnosis to determine the presence of antibodies to the microbe. Therefore, demonstration of the presence of an infectious agent is often a necessary criterion for the diagnosis of the disease. This is not the case for bacterial vaginosis, since the ultimate cause of the disease is not yet known. There are a variety of methods for the diagnosis of bacterial vaginosis but no method can at present be regarded as the best. Diagnosing bacterial vaginosis has long been based on the clinical criteria of Amsel, whereby three of four defined criteria must be satisfied. Nugent’s scoring system has been further developed and includes validation of the categories of observable bacteria structures. Up­to­date molecular tests are introduced, and better understanding of vaginal microbiome, a clear definition for bacterial vaginosis, and short­term and long­term fluctuations in vaginal microflora will help to better define molecular tests within the broader clinical context.

  3. Adult bacterial meningitis

    DEFF Research Database (Denmark)

    Meyer, C N; Samuelsson, I S; Galle, M


    Episodes of adult bacterial meningitis (ABM) at a Danish hospital in 1991-2000 were identified from the databases of the Department of Clinical Microbiology, and compared with data from the Danish National Patient Register and the Danish National Notification System. Reduced penicillin susceptibi......Episodes of adult bacterial meningitis (ABM) at a Danish hospital in 1991-2000 were identified from the databases of the Department of Clinical Microbiology, and compared with data from the Danish National Patient Register and the Danish National Notification System. Reduced penicillin...

  4. Bacterial blight of cotton

    Directory of Open Access Journals (Sweden)

    Aïda JALLOUL


    Full Text Available Bacterial blight of cotton (Gossypium ssp., caused by Xanthomonas citri pathovar malvacearum, is a severe disease occurring in all cotton-growing areas. The interactions between host plants and the bacteria are based on the gene-for-gene concept, representing a complex resistance gene/avr gene system. In light of the recent data, this review focuses on the understanding of these interactions with emphasis on (1 the genetic basis for plant resistance and bacterial virulence, (2 physiological mechanisms involved in the hypersensitive response to the pathogen, including hormonal signaling, the oxylipin pathway, synthesis of antimicrobial molecules and alteration of host cell structures, and (3 control of the disease.

  5. Bacterial meningitis in infants. (United States)

    Ku, Lawrence C; Boggess, Kim A; Cohen-Wolkowiez, Michael


    Neonatal bacterial meningitis is uncommon but devastating. Morbidity among survivors remains high. The types and distribution of pathogens are related to gestational age, postnatal age, and geographic region. Confirming the diagnosis is difficult. Clinical signs are often subtle, lumbar punctures are frequently deferred, and cerebrospinal fluid (CSF) cultures can be compromised by prior antibiotic exposure. Infants with bacterial meningitis can have negative blood cultures and normal CSF parameters. Promising tests such as the polymerase chain reaction require further study. Prompt treatment with antibiotics is essential. Clinical trials investigating a vaccine for preventing neonatal Group B Streptococcus infections are ongoing. Copyright © 2015 Elsevier Inc. All rights reserved.

  6. Coeficientes de digestibilidad total y de proteínas en alimentos experimentales para juveniles de Oplegnathus insignis (Kner, 1867 (Perciformes, Oplegnathidae

    Directory of Open Access Journals (Sweden)

    Avelino Muñoz


    Full Text Available Los alimentos para peces están formulados con diversos ingredientes, donde la harina de pescado es la principal fuente de proteína. Sin embargo, este insumo tiene un alto costo que lleva a la necesidad de evaluar nuevas fuentes de proteínas y probarlas en especies marinas. Oplegnathus insignis (San Pedro, Pacific beakfish es una especie omnívora en su etapa intermareal y carnívora en su etapa submareal, que ha sido cultivada de manera experimental, siendo necesario incorporar dietas formuladas. Este estudio reporta la experiencia en alimentación y nutrición, de juveniles de O. insignis alimentados con dietas formuladas con distintas fuentes de proteína. Se efectuó una experiencia con juveniles de O. insignis provenientes de un cultivo experimental. Se formularon cuatro alimentos, uno en base de harina de pescado (referencia y tres modificando la fuente de proteína del alimento de referencia, intercambiando un 30% de harina de pescado, por harina de soya, harina de moluscos y harina de quinoa. Se experimentó con 180 ind de 295,6 g y de 450 días post-eclosión, distribuidos en 12 estanques rectangulares de 1,6 m³. La alimentación fue a saciedad. Se efectuaron análisis próximales de los alimentos experimentales de las heces colectadas. Se determinó los coeficientes de digestibilidad total y proteína. El alimento con mayor contenido de proteína fue el que contenía harina de moluscos. El mayor coeficiente de digestibilidad total lo tienen los alimentos con harina de pescado (68,0% y harina de moluscos (67,1%, mientras que en los alimentos en base a harinas vegetales, la digestibilidad total para la harina de soya fue de 62,7% y para harina de quinoa de 64,1%. Estos resultados indican que es factible reemplazar un 30% de la harina de pescado por harinas de origen vegetal.

  7. Investigation experimentale de l'ecoulement et des performances thermiques du nanofluide eau-oxyde de cuivre dans un Micro-Canal Chauffe (United States)

    Rimbault, Benjamin

    Cette these de maitrise presentee par articles visait a etudier le comportement hydraulique et thermique d'un ecoulement de nanofluides en micro-canal chauffe. Nous avons etudie premierement de l'eau distillee, ensuite des melanges de particules d'oxyde de cuivre (taille 29nm) avec de l'eau distillee en concentrations particulaires volumiques 4.5%, 1.03%, et 0.24% (CuO-H2O). L'ecoulement force des differents fluides a ete realise au moyen de pompes a engrenages au sein d'un circuit ferme, comprenant un micro-canal a section rectangulaire (e=1.116mm,1=25.229mm) chauffe sur deux faces paralleles via des cartouches electriques, deux echangeurs de chaleurs en serie, ainsi qu'un debitmetre magnetique. A notre connaissance peu d'etudes sur l'ecoulement de nanofluides d'oxyde de cuivre-eau en micro-canal rectangulaire chauffe sont disponibles dans la litterature, cette recherche sert de contribution. Premierement, une validation avec la litterature a ete effectuee pour le cas d'un ecoulement d'eau entre plaques planes paralleles chauffees. Des essais hydrauliques ont ete realises pour une gamme du nombre de Reynolds allant jusqu'a Re=5000 a temperature constante. Par la suite des essais thermiques jusqu'a Re=2500 ont consiste en une elevation de temperature fixe (20.5°C a 30.5°C) a travers la longueur du micro-canal sous un regime stationnaire. Les resultats ont demontre une augmentation de la perte de pression et du coefficient de frottement des nanofluides sur l'eau pour un meme debit. Une telle augmentation de perte de pression etait de +70%, +25%, et +0 a 30% respectivement pour les concentrations 4.50%, 1.03%, et 0.24%. Concernant la transition laminaire a turbulent les comportements semblaient indiquer une valeur critique semblable entre l'eau et les differentes concentrations avec et sans chauffage a un nombre de Reynolds critique Rem 1000. Nous avons observe une legere augmentation du coefficient de convection thermique avec le debit massique pour les faibles

  8. The Bacterial Growth Curve. (United States)

    Paulton, Richard J. L.


    A procedure that allows students to view an entire bacterial growth curve during a two- to three-hour student laboratory period is described. Observations of the lag phase, logarithmic phase, maximum stationary phase, and phase of decline are possible. A nonpathogenic, marine bacterium is used in the investigation. (KR)

  9. Bacterial fingerprints across Europe

    NARCIS (Netherlands)

    Glasner, Corinna


    Bacterial pathogens, such as Staphylococcus aureus and carbapenemase-producing Enterobacteriaceae (CPE), impose major threats to human health worldwide. Both have a ‘Jekyll & Hyde’ character, since they can be present as human commensals, but can also become harmful invasive pathogens especially

  10. [Bacterial biofilms and infection]. (United States)

    Lasa, I; Del Pozo, J L; Penadés, J R; Leiva, J


    In developed countries we tend to think of heart disease and the numerous forms of cancer as the main causes of mortality, but on a global scale infectious diseases come close, or may even be ahead: 14.9 million deaths in 2002 compared to cardiovascular diseases (16.9 million deaths) and cancer (7.1 million deaths) (WHO report 2004). The infectious agents responsible for human mortality have evolved as medical techniques and hygienic measures have changed. Modern-day acute infectious diseases caused by specialized bacterial pathogens such as diphtheria, tetanus, cholera, plague, which represented the main causes of death at the beginning of XX century, have been effectively controlled with antibiotics and vaccines. In their place, more than half of the infectious diseases that affect mildly immunocompromised patients involve bacterial species that are commensal with the human body; these can produce chronic infections, are resistant to antimicrobial agents and there is no effective vaccine against them. Examples of these infections are the otitis media, native valve endocarditis, chronic urinary infections, bacterial prostatitis, osteomyelitis and all the infections related to medical devices. Direct analysis of the surface of medical devices or of tissues that have been foci of chronic infections shows the presence of large numbers of bacteria surrounded by an exopolysaccharide matrix, which has been named the "biofilm". Inside the biofilm, bacteria grow protected from the action of the antibodies, phagocytic cells and antimicrobial treatments. In this article, we describe the role of bacterial biofilms in human persistent infections.


    African Journals Online (AJOL)


    Spontaneous bacterial peritonitis (SBP) frequent]y occurs in patients with liver cirrhosis and ascites. It is defined as an infection of previously sterile ascitic fluid without any demonstrable intrabdominal source of infection. It is now internationally agreed that a polymorphonuclear (PMN) cell count in the ascitic fluid of over 250 ...

  12. Bacterial membrane proteomics. (United States)

    Poetsch, Ansgar; Wolters, Dirk


    About one quarter to one third of all bacterial genes encode proteins of the inner or outer bacterial membrane. These proteins perform essential physiological functions, such as the import or export of metabolites, the homeostasis of metal ions, the extrusion of toxic substances or antibiotics, and the generation or conversion of energy. The last years have witnessed completion of a plethora of whole-genome sequences of bacteria important for biotechnology or medicine, which is the foundation for proteome and other functional genome analyses. In this review, we discuss the challenges in membrane proteome analysis, starting from sample preparation and leading to MS-data analysis and quantification. The current state of available proteomics technologies as well as their advantages and disadvantages will be described with a focus on shotgun proteomics. Then, we will briefly introduce the most abundant proteins and protein families present in bacterial membranes before bacterial membrane proteomics studies of the last years will be presented. It will be shown how these works enlarged our knowledge about the physiological adaptations that take place in bacteria during fine chemical production, bioremediation, protein overexpression, and during infections. Furthermore, several examples from literature demonstrate the suitability of membrane proteomics for the identification of antigens and different pathogenic strains, as well as the elucidation of membrane protein structure and function.

  13. Seizures Complicating Bacterial Meningitis

    Directory of Open Access Journals (Sweden)

    J Gordon Millichap


    Full Text Available The clinical data of 116 patients, 1 month to <5 years of age, admitted for bacterial meningitis, and grouped according to those with and without seizures during hospitalization, were compared in a study at Buddhist Dalin Tzu Chi General Hospital, Chang Gung Memorial Hospital and other centers in Taiwan.

  14. Diagnosis of bacterial infection

    African Journals Online (AJOL)

    rapid and easy-to-use test for bacterial infections. Clearly, this is a very ... detect antigens or specific antibodies, e.g. group A streptococcal antigen testing can be employed to reduce antibiotic use. Culture-based tests are often ... White blood cell count 12 000 cells/mm³; or the presence of >10% ...

  15. Bacterial Meningitis Outcome


    J Gordon Millichap


    The neurologic, psychological, and educational outcomes of bacterial meningitis in 130 children evaluated at a mean age of 8 years, and 6 years after their meningitis, are reported from the Department of Paediatrics and Clinical Epidemiology and Biostatistics Unit, University of Melbourne, and the Royal Children’s Hospital, Victoria, Australia.

  16. Corticosteroids for Bacterial Keratitis (United States)

    Srinivasan, Muthiah; Mascarenhas, Jeena; Rajaraman, Revathi; Ravindran, Meenakshi; Lalitha, Prajna; Glidden, David V.; Ray, Kathryn J.; Hong, Kevin C.; Oldenburg, Catherine E.; Lee, Salena M.; Zegans, Michael E.; McLeod, Stephen D.; Lietman, Thomas M.; Acharya, Nisha R.


    Objective To determine whether there is a benefit in clinical outcomes with the use of topical corticosteroids as adjunctive therapy in the treatment of bacterial corneal ulcers. Methods Randomized, placebo-controlled, double-masked, multicenter clinical trial comparing prednisolone sodium phosphate, 1.0%, to placebo as adjunctive therapy for the treatment of bacterial corneal ulcers. Eligible patients had a culture-positive bacterial corneal ulcer and received topical moxifloxacin for at least 48 hours before randomization. Main Outcome Measures The primary outcome was best spectacle-corrected visual acuity (BSCVA) at 3 months from enrollment. Secondary outcomes included infiltrate/scar size, reepithelialization, and corneal perforation. Results Between September 1, 2006, and February 22, 2010, 1769 patients were screened for the trial and 500 patients were enrolled. No significant difference was observed in the 3-month BSCVA (−0.009 logarithm of the minimum angle of resolution [logMAR]; 95% CI, −0.085 to 0.068; P = .82), infiltrate/scar size (P = .40), time to reepithelialization (P = .44), or corneal perforation (P > .99). A significant effect of corticosteroids was observed in subgroups of baseline BSCVA (P = .03) and ulcer location (P = .04). At 3 months, patients with vision of counting fingers or worse at baseline had 0.17 logMAR better visual acuity with corticosteroids (95% CI, −0.31 to −0.02; P = .03) compared with placebo, and patients with ulcers that were completely central at baseline had 0.20 logMAR better visual acuity with corticosteroids (−0.37 to −0.04; P = .02). Conclusions We found no overall difference in 3-month BSCVA and no safety concerns with adjunctive corticosteroid therapy for bacterial corneal ulcers. Application to Clinical Practice Adjunctive topical corticosteroid use does not improve 3-month vision in patients with bacterial corneal ulcers. PMID:21987582

  17. Determination experimentale des principales caracteristiques ...

    African Journals Online (AJOL)

    Through the present research work, usual scientific parameters and characteristics of Borassus palm wood from Benin, are determined. Specifically, infradensity, shrinkage coefficient and behaviour, breaking stress in the direction of the fibbers at pulling, compression and bending, are known as well as Young modulus at ...

  18. Radiometric detection of bacterial metabolism

    International Nuclear Information System (INIS)

    Camargo, E.E.; Wagner Junior, H.N.


    The measurement of 14 CO 2 produced by the bacterial oxidation of labelled compounds is discussed as a means of evaluating the bacterial metabolism. The following items are discussed:automated radiometric detection, types of graphs, clinical applications of the radiometric system and influential factors. Complementary studies on bacterial assimilation of substances are presented. (M.A.) [pt

  19. Pharmacological study of radioactive-gold colloid transport by blood and by serous exudate; Contribution a l'etude pharmacologique du transport des colloides d'or radioactif par le sang et les exsudats sereux

    Energy Technology Data Exchange (ETDEWEB)

    Rousselet, J. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    substances et particulierement dans le transport de l'or colloidal radioactif. Les fractions sanguines qui peuvent participer au transport de l'or colloidal sont les hematies, les leucocytes et elements histiocytaires, et le plasma. La repartition de la radioactivite dans ces elements est precisee en pratiquant des autoradiographies de coupes de culots de sang centrifuge et congele apres incubation avec de l'or colloidal. Cette etude montre l'importance du plasma dans le transport des particules radioactives. Nous avons alors recherche une fixation eventuelle de l'or colloidal 198 sur les diverses proteines seriques en appliquant certaines methodes de separation a savoir une filtration-exclusion sur colonne de gel de Sephadex G-200 et un fractionnement electrophoretique sur papier et sur acetate de cellulose gelatineux (cellogel). Nous avons etudie la liaison ''in vitro'' et ''in vivo'' des particules colloidales d'or 198 d'abord avec les proteines seriques de sujets sains puis avec les proteines des liquides d'epanchement d'origine pathologique, chez l'homme, ou du a une inflammation experimentale a la carragenine chez le rat. Les particules d'or colloidal 198 de 30 m{mu} (S-2) sont liees dans une certaine mesure aux {alpha}2 globulines sanguines ou ascitiques, celles de 5 m{mu} (S-6) sont liees aux {alpha}1 globulines de meme que les grains contenus dans l'or polydisperse. Cette liaison semble intervenir grace a la couche protectrice de macromolecules constituees par la gelatine. Seules les caracteristiques physicochimiques de grains colloidaux peuvent expliquer leur difference de localisation sur l'electrophoregramme. L'or sous forme ionique, par contre, est combine, aux seules albumines si la quantite de metal presente ne depasse pas une certaine limite. (auteur)

  20. Bacterial Cell Wall Components (United States)

    Ginsberg, Cynthia; Brown, Stephanie; Walker, Suzanne

    Bacterial cell-surface polysaccharides cells are surrounded by a variety of cell-surface structures that allow them to thrive in extreme environments. Components of the cell envelope and extracellular matrix are responsible for providing the cells with structural support, mediating intercellular communication, allowing the cells to move or to adhere to surfaces, protecting the cells from attack by antibiotics or the immune system, and facilitating the uptake of nutrients. Some of the most important cell wall components are polysaccharide structures. This review discusses the occurrence, structure, function, and biosynthesis of the most prevalent bacterial cell surface polysaccharides: peptidoglycan, lipopolysaccharide, arabinogalactan, and lipoarabinomannan, and capsular and extracellular polysaccharides. The roles of these polysaccharides in medicine, both as drug targets and as therapeutic agents, are also described.

  1. Bacterial meningitis in Nottingham.


    Ispahani, P.


    Records of 171 cases of bacterial meningitis admitted to Nottingham hospitals from January 1974 to June 1980 were reviewed. The distribution of organisms producing meningitis and the factors influencing mortality in different age groups were assessed. Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae accounted for 69% of all proven cases. The overall mortality was 26% being lowest in patients with meningococcal meningitis (0%) and highest in those with pneumococcal m...

  2. Neglected bacterial zoonoses. (United States)

    Chikeka, I; Dumler, J S


    Bacterial zoonoses comprise a group of diseases in humans or animals acquired by direct contact with or by oral consumption of contaminated animal materials, or via arthropod vectors. Among neglected infections, bacterial zoonoses are among the most neglected given emerging data on incidence and prevalence as causes of acute febrile illness, even in areas where recognized neglected tropical diseases occur frequently. Although many other bacterial infections could also be considered in this neglected category, five distinct infections stand out because they are globally distributed, are acute febrile diseases, have high rates of morbidity and case fatality, and are reported as commonly as malaria, typhoid or dengue virus infections in carefully designed studies in which broad-spectrum diagnoses are actively sought. This review will focus attention on leptospirosis, relapsing fever borreliosis and rickettsioses, including scrub typhus, murine typhus and spotted fever group rickettsiosis. Of greatest interest is the lack of distinguishing clinical features among these infections when in humans, which confounds diagnosis where laboratory confirmation is lacking, and in regions where clinical diagnosis is often attributed to one of several perceived more common threats. As diseases such as malaria come under improved control, the real impact of these common and under-recognized infections will become evident, as will the requirement for the strategies and allocation of resources for their control. Copyright © 2015 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

  3. Bacterial growth kinetics

    International Nuclear Information System (INIS)

    Boonkitticharoen, V.; Ehrhardt, J.C.; Kirchner, P.T.


    Quantitative measurement of bacterial growth may be made using a radioassay technique. This method measures, by scintillation counting, the 14 CO 2 derived from the bacterial metabolism of a 14 C-labeled substrate. Mathematical growth models may serve as reliable tools for estimation of the generation rate constant (or slope of the growth curve) and provide a basis for evaluating assay performance. Two models, i.e., exponential and logistic, are proposed. Both models yielded an accurate fit to the data from radioactive measurement of bacterial growth. The exponential model yielded high precision values of the generation rate constant, with an average relative standard deviation of 1.2%. Under most conditions the assay demonstrated no changes in the slopes of growth curves when the number of bacteria per inoculation was changed. However, the radiometric assay by scintillation method had a growth-inhibiting effect on a few strains of bacteria. The source of this problem was thought to be hypersensitivity to trace amounts of toluene remaining on the detector

  4. Aplicación de modelos lineales mixtos en infecciones experimentales con WSSV en el camarón blanco Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    María Alejandra Ramírez-Ruiz


    Full Text Available El virus de la mancha blanca (WSSV es uno de los virus más devastadores en la industria camaronícola. Hasta la fecha no se ha hallado una cura para la enfermedad por lo que es necesario diseñar protocolos experimentales reproducibles y medibles para evaluar fármacos y respuestas fisiológicas de los ejemplares durante la prognosis de la infección. En este estudio se evaluaron dos vías de infección (inyección e inmersión con WSSV (200 copias de ADN a temperatura constante de 26 ± 0,5°C, en juveniles Litopenaeus vannamei (4,8 ± 0,38 g en estado de inter-muda. En la infección por inyección se observó nado errático, letargia y coloración rojiza a partir de las 24 h y la mortalidad fue del 100% a los 2-5 días: en 63% de los ejemplares se observó infección ligera [20 copias de ADN y 1-5 cuerpos de inclusión intranuclear (CAI/200 campos], 21% con infección moderada (200 copias de ADN y 1-2 CAI/20 campos y 16% con infección severa (2000 copias de ADN y más de 10 CAI/campo. No se observó mortalidad en los ejemplares controles. En la infección por inmersión, los signos de la enfermedad se observaron a partir del día 3, en un período de 3-9 días se observó 38% de mortalidad: 25% con infección ligera (20 copias de ADN y 1-2 CAI/20 campos, 5% con infección moderada (200 copias de ADN y 1-2 CAI/ 20 campos y 8% con infección severa (2000 copias de ADN y 1-5 CAI/2 campos. El 62% que sobrevivió, fue PCR positivo, con grado de infección ligera (20 copias de ADN pero sin inclusiones CAI. No se observó mortalidad en el grupo control. Los datos recabados no cumplían con los supuestos de independencia y linealidad requeridos para la aplicación de análisis de varianza por lo que se usaron los modelos lineales mixtos donde se observó mayor precisión y capacidad de predicción. En los ejemplares inyectados la mortalidad tuvo su máximo más alto al día 5 después de la inoculación. Mientras que en los infectados por inmersi

  5. Estrategias experimentales para el estudio de las propiedades de la proteína Core del virus de la hepatitis C

    Directory of Open Access Journals (Sweden)

    Gonzálo Correa Arango


    Full Text Available

    El modelo de infección por el Virus de la Hepatitis C (VHC se ha convertido en el tópico de interés de numerosos equipos de investigación, considerando el alto porcentaje de infección persistente asociada al VHC. En efecto, del 50 al 80% de los pacientes con infección por el VHC, desarrollan infección persistente, que puede evolucionar a cirrosis y carcinoma hepatocelular (HCC. Este modelo presenta dos obstáculos mayores para su estudio: la ausencia de un sistema eficiente de replicación viral in vitro y el limitado número de modelos animales.

    La proteína Core del VHC, además de ser la unidad estructural de la cápside viral, parece estar implicada en las estrategias virales de persistencia y oncogénesis; nuestro grupo ha planteado estrategias experimentales para estudiar algunas de sus propiedades, tales como su capacidad inmunonoduladora en cultivos de cálulas dendríticas humanas, su capacidad de modificar la expresión del ARNm en células HepG2 con expresión transitoria de Core y la presencia de esta proteína en tejido hepático proveniente de pacientes con diagnóstico de HCC; dichas estrategias experimentales han sido:

    Producción de la proteína Core del VHC en el sistema baculovirus y purificación en condiciones nativas para su evaluación en ensayos biológicos.

    Expresión transitoria de la proteína Core del VHC en la línea celular HepG2, mediante transducción con partículas recombinantes del Virus Semliki Forest (SFV.

    Estudio de la expresión de p53

  6. Classic, fuzzy and predictive dtc strategies for the PMSM using the bacterial foraging algorithmas an online parameter estimator

    Directory of Open Access Journals (Sweden)

    Gabriel Noriega


    Full Text Available Este trabajo presenta una comparación entre cuatro técnicas de control directo de par (DTC aplicadas al accionamiento de la máquina sincrónica deimán permanente: Clásico, Modificado con un sistema de inferencia difusa, Predictivo y Predictivo con inferencia difusa. La estimación de parámetros para las estrategias predictivas es realizado utilizando un algoritmo de búsqueda poblacional basado en Forraje Bacterial, el cuál es capaz de calcular en línea los parámetros de la máquina sincrónica de imán permanente. Los resultados experimentales del par eléctrico y de los enlaces de flujo del estator muestran mejoras significativas para las estrategias predictivas que utilizan algoritmos de estimación paramétrica mediante Forraje Bacterial cuando se comparan con técnicas no predictivas.

  7. Etude des propriétés magnéto-optiques des couches minces à base ...

    African Journals Online (AJOL)

    Etude des propriétés magnéto-optiques des couches minces à base de ferrite de cobalt obtenues par voie sol-gel. ... Les courbes obtenues à la longueur d'onde de 820 nm présentent des cycles d'hystérésis avec des rotations rémanentes de 40, 60 et 81°/cm. Ces résultats présentent une excellente rotation Faraday de ...

  8. Radiology of bacterial pneumonia

    Energy Technology Data Exchange (ETDEWEB)

    Vilar, Jose E-mail:; Domingo, Maria Luisa; Soto, Cristina; Cogollos, Jonathan


    Bacterial pneumonia is commonly encountered in clinical practice. Radiology plays a prominent role in the evaluation of pneumonia. Chest radiography is the most commonly used imaging tool in pneumonias due to its availability and excellent cost benefit ratio. CT should be used in unresolved cases or when complications of pneumonia are suspected. The main applications of radiology in pneumonia are oriented to detection, characterisation and follow-up, especially regarding complications. The classical classification of pneumonias into lobar and bronchial pneumonia has been abandoned for a more clinical classification. Thus, bacterial pneumonias are typified into three main groups: Community acquired pneumonia (CAD), Aspiration pneumonia and Nosocomial pneumonia (NP).The usual pattern of CAD is that of the previously called lobar pneumonia; an air-space consolidation limited to one lobe or segment. Nevertheless, the radiographic patterns of CAD may be variable and are often related to the causative agent. Aspiration pneumonia generally involves the lower lobes with bilateral multicentric opacities. Nosocomial Pneumonia (NP) occurs in hospitalised patients. The importance of NP is related to its high mortality and, thus, the need to obtain a prompt diagnosis. The role of imaging in NP is limited but decisive. The most valuable information is when the chest radiographs are negative and rule out pneumonia. The radiographic patterns of NP are very variable, most commonly showing diffuse multifocal involvement and pleural effusion. Imaging plays also an important role in the detection and evaluation of complications of bacterial pneumonias. In many of these cases, especially in hospitalised patients, chest CT must be obtained in order to better depict these associate findings.

  9. Radiology of bacterial pneumonia

    International Nuclear Information System (INIS)

    Vilar, Jose; Domingo, Maria Luisa; Soto, Cristina; Cogollos, Jonathan


    Bacterial pneumonia is commonly encountered in clinical practice. Radiology plays a prominent role in the evaluation of pneumonia. Chest radiography is the most commonly used imaging tool in pneumonias due to its availability and excellent cost benefit ratio. CT should be used in unresolved cases or when complications of pneumonia are suspected. The main applications of radiology in pneumonia are oriented to detection, characterisation and follow-up, especially regarding complications. The classical classification of pneumonias into lobar and bronchial pneumonia has been abandoned for a more clinical classification. Thus, bacterial pneumonias are typified into three main groups: Community acquired pneumonia (CAD), Aspiration pneumonia and Nosocomial pneumonia (NP).The usual pattern of CAD is that of the previously called lobar pneumonia; an air-space consolidation limited to one lobe or segment. Nevertheless, the radiographic patterns of CAD may be variable and are often related to the causative agent. Aspiration pneumonia generally involves the lower lobes with bilateral multicentric opacities. Nosocomial Pneumonia (NP) occurs in hospitalised patients. The importance of NP is related to its high mortality and, thus, the need to obtain a prompt diagnosis. The role of imaging in NP is limited but decisive. The most valuable information is when the chest radiographs are negative and rule out pneumonia. The radiographic patterns of NP are very variable, most commonly showing diffuse multifocal involvement and pleural effusion. Imaging plays also an important role in the detection and evaluation of complications of bacterial pneumonias. In many of these cases, especially in hospitalised patients, chest CT must be obtained in order to better depict these associate findings

  10. Bacterial Degradation of Pesticides

    DEFF Research Database (Denmark)

    Knudsen, Berith Elkær

    This PhD project was carried out as part of the Microbial Remediation of Contaminated Soil and Water Resources (MIRESOWA) project, funded by the Danish Council for Strategic Research (grant number 2104-08-0012). The environment is contaminated with various xenobiotic compounds e.g. pesticides......D student, to construct fungal-bacterial consortia in order to potentially stimulate pesticide degradation thereby increasing the chance of successful bioaugmentation. The results of the project are reported in three article manuscripts, included in this thesis. In manuscript I, the mineralization of 2...

  11. Bacterial mitotic machineries

    DEFF Research Database (Denmark)

    Gerdes, Kenn; Møller-Jensen, Jakob; Ebersbach, Gitte


    Here, we review recent progress that yields fundamental new insight into the molecular mechanisms behind plasmid and chromosome segregation in prokaryotic cells. In particular, we describe how prokaryotic actin homologs form mitotic machineries that segregate DNA before cell division. Thus, the Par......M protein of plasmid R1 forms F actin-like filaments that separate and move plasmid DNA from mid-cell to the cell poles. Evidence from three different laboratories indicate that the morphogenetic MreB protein may be involved in segregation of the bacterial chromosome....

  12. Changes in bacterial meningitis.


    Carter, P E; Barclay, S M; Galloway, W H; Cole, G F


    In 1964, one of us (WHG) undertook a retrospective study of bacterial meningitis in childhood in the north east of Scotland during the period 1946-61. We have recently carried out a similar review of cases occurring during 1971-86, to compare the incidence, mortality, and bacteriological patterns. During the earlier period 285 cases occurred, a total incidence of 16.9/100,000 children per year. In the later period 274 children were affected, an annual incidence of 17.8/100,000. The overall mo...

  13. Technological study of organic fluids; Etudes technologiques des fluides caloporteurs organique

    Energy Technology Data Exchange (ETDEWEB)

    Leveque, P. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    Under contract to EURATOM (MM. VAN der VENNE and HANNAERT), the C.E.A. and the Progil Society have collaborated on a technological study of the terphenyls. The results obtained from the radiolysis and pyrolysis of the product OM2 (70 p. 100 m-terphenyl, 25 p. 100 o-terphenyl, 5 p. 100 p-terphenyl) are reported. Using two pile loops at temperatures up to 450 deg. C, a phenomena of accelerated pyrolysis on the radiolized material has been demonstrated. Some by-products of the petroleum industry have also been studied, with a view to finding substitutes for terphenyl. (author) [French] Le C.E.A. et la Societe PROGIL ont entrepris en commun sous contrat EURATOM (MM. VAN der VENNE et HANNAERT) une etude technologique des terphenyles. Les resultats de radiolyse et de pyrolyse obtenus sur le produit OM2 (70 p. 100 m-terphenyle, 25 p. 100 o-terphenyle. 5 p. 100 de p-terphenyle) sont exposes. L'exploitation de deux boucles en pile jusqu'a des temperatures de 450 deg. C a mis en evidence un phenomene de pyrolyse acceleree sur le produit radiolyse. On a etudie egalement quelques sous-produits de l'industrie petroliere dans le but de trouver des substituts au terphenyle. (auteur)

  14. Preparation and study of dialkyl nitroxide radicals; Preparation et etude de radicaux nitroxydes diacyles

    Energy Technology Data Exchange (ETDEWEB)

    Chenavas, P. [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires


    These radicals are obtained by oxidation of N-hydroxy-imides with lead tetracetate or p-nitro-perbenzoic acid. These imides are prepared by heating dicarboxylic acids anhydrides with benzyloxy-amine followed by catalytic hydrogenation of N-benzyloxy-imides so obtained. Two series of radicals have mainly been studied, the first concerning five-membered cyclic imides, the second six-membered cyclic imides, these molecules having methyls substituents or no on the carbon ring. N. M. R. spectra of some O-benzyl-imides have been analysed. These different results have made it possible to study the conformation and stereochemistry of these imides. (author) [French] Ces radicaux sont obtenus par oxydation d'imides N-hydroxyles par le tetracetate de plomb ou l'acide p-nitroperbenzoique; ces imides sont prepares par chauffage d'anhydrides de diacides en presence de benzyloxyamine suivie d'une hydrogenation catalytique des N-benzyloxyimides ainsi obtenus. Deux series de radicaux ont principalement ete etudies: la premiere relative a des imides cycliques a cinq chainons, la seconde a des imides cycliques a six chainons, ces molecules ayant des substituants methyles ou non sur la chaine carbonee. Les derives O-benzyles de quelques-uns de ces imides ont ete analyses en R. M. N. Ces differents resultats ont permis une etude de la conformation et de la stereochimie de ces imides. (auteur)

  15. Capacidad para tomar decisiones frente al Consentimiento Informado de personas que potencialmente participarían en estudios clínicos experimentales para la industria farmacéutica. Barranquilla (Colombia. Mayo-noviembre de 2009

    Directory of Open Access Journals (Sweden)

    Gloria Cecilia Visbal Illera


    Full Text Available Objetivo: Determinar el grado de capacidad para tomar decisiones frente al consentimiento informado de personas que potencialmente participarian en ensayos clínicos experimentales para la industria farmacéutica en la ciudad de Barranquilla. Materiales y métodos: Estudio descriptivo transversal, muestra de 100 pacientes con diagnóstico de hipertensión arterial y diabetes mellitus Tipo II; se aplicó el consentimiento informado. Se utilizó el instrumento de evaluación de competencias Competence Assessment Toolfor Clinical Research MacCAT-CR. Se calcularon estadísticas descriptivas y pruebas no paramétricas, como la prueba Ji cuadrada y la prueba de la mediana; se decidió con base en los valores de p de las respectivas pruebas. Resultados: No se encontró entendimiento de la naturaleza del estudio; ninguno obtuvo puntaje del 100% en las preguntas formuladas. El 76% de los participantes mostró un menor entendimiento de la naturaleza y propósito del estudio y un 84% no comprendió que podrían verse afectados, sin embargo, la mayoría participó. El 62% no entendió que el propósito del investigador no era proporcionar medicación, sino investigar eficacia, tolerancia y efectos de un medicamento en estudio en los seres humanos. Conclusiones: La autonomía para decidir participar en los estudios clínicos experimentales se debe basar en la confianza, cooperación, ética, diálogo, apertura al cambio y la regulación colectiva, entendiendo el proceso de consentir, aquel en que la información trasciende más allá que la de un simple formato que busca la aceptación y legalización de la información que se ha suministrado.

  16. Animal Models of Bacterial Keratitis (United States)

    Marquart, Mary E.


    Bacterial keratitis is a disease of the cornea characterized by pain, redness, inflammation, and opacity. Common causes of this disease are Pseudomonas aeruginosa and Staphylococcus aureus. Animal models of keratitis have been used to elucidate both the bacterial factors and the host inflammatory response involved in the disease. Reviewed herein are animal models of bacterial keratitis and some of the key findings in the last several decades. PMID:21274270

  17. Aerotaxis in Bacterial Turbulence (United States)

    Fernandez, Vicente; Bisson, Antoine; Bitton, Cindy; Waisbord, Nicolas; Smriga, Steven; Rusconi, Roberto; Stocker, Roman


    Concentrated suspensions of motile bacteria exhibit correlated dynamics on spatial scales much larger than an individual bacterium. The resulting flows, visually similar to turbulence, can increase mixing and decrease viscosity. However, it remains unclear to what degree the collective dynamics depend on the motile behavior of bacteria at the individual level. Using a new microfluidic device to create controlled horizontal oxygen gradients, we studied the two dimensional behavior of dense suspensions of Bacillus subtilis. This system makes it possible to assess the interplay between the coherent large-scale motions of the suspension, oxygen transport, and the directional response of cells to oxygen gradients (aerotaxis). At the same time, this device has enabled us to examine the onset of bacterial turbulence and its influence on the propagation of the diffusing oxygen front, as the bacteria begin in a dormant state and transition to swimming when exposed to oxygen.

  18. Bacterial intermediate filaments

    DEFF Research Database (Denmark)

    Charbon, Godefroid; Cabeen, M.; Jacobs-Wagner, C.


    Crescentin, which is the founding member of a rapidly growing family of bacterial cytoskeletal proteins, was previously proposed to resemble eukaryotic intermediate filament (IF) proteins based on structural prediction and in vitro polymerization properties. Here, we demonstrate that crescentin...... also shares in vivo properties of assembly and dynamics with IF proteins by forming stable filamentous structures that continuously incorporate subunits along their length and that grow in a nonpolar fashion. De novo assembly of crescentin is biphasic and involves a cell size-dependent mechanism...... a new function for MreB and providing a parallel to the role of actin in IF assembly and organization in metazoan cells. Additionally, analysis of an MreB localization mutant suggests that cell wall insertion during cell elongation normally occurs along two helices of opposite handedness, each...

  19. Bacterial polyhydroxyalkanoates: Still fabulous? (United States)

    Możejko-Ciesielska, Justyna; Kiewisz, Robert


    Bacterial polyhydroxyalkanoates (PHA) are polyesters accumulated as carbon and energy storage materials under limited growth conditions in the presence of excess carbon sources. They have been developed as biomaterials with unique properties for the past many years being considered as a potential substitute for conventional non-degradable plastics. Due to the increasing concern towards global climate change, depleting petroleum resource and problems with an utilization of a growing number of synthetic plastics, PHAs have gained much more attention from industry and research. These environmentally friendly microbial polymers have great potential in biomedical, agricultural, and industrial applications. However, their production on a large scale is still limited. This paper describes the backgrounds of PHAs and discussed the current state of knowledge on the polyhydroxyalkanoates. Ability of bacteria to convert different carbon sources to PHAs, the opportunities and challenges of their introduction to global market as valuable renewable products have been also discussed. Copyright © 2016 Elsevier GmbH. All rights reserved.

  20. Biosensors of bacterial cells. (United States)

    Burlage, Robert S; Tillmann, Joshua


    Biosensors are devices which utilize both an electrical component (transducer) and a biological component to study an environment. They are typically used to examine biological structures, organisms and processes. The field of biosensors has now become so large and varied that the technology can often seem impenetrable. Yet the principles which underlie the technology are uncomplicated, even if the details of the mechanisms are elusive. In this review we confine our analysis to relatively current advancements in biosensors for the detection of whole bacterial cells. This includes biosensors which rely on an added labeled component and biosensors which do not have a labeled component and instead detect the binding event or bound structure on the transducer. Methods to concentrate the bacteria prior to biosensor analysis are also described. The variety of biosensor types and their actual and potential uses are described. Copyright © 2016 Elsevier B.V. All rights reserved.

  1. Bacterial proteases and virulence

    DEFF Research Database (Denmark)

    Frees, Dorte; Brøndsted, Lone; Ingmer, Hanne


    with the proteases either encoded within the same polypeptide or on separate subunits. In contrast, substrate recognition by extracellular proteases is less selective and therefore these enzymes are generally expressed as zymogens to prevent premature proteolytic activity that would be detrimental to the cell......Bacterial pathogens rely on proteolysis for variety of purposes during the infection process. In the cytosol, the main proteolytic players are the conserved Clp and Lon proteases that directly contribute to virulence through the timely degradation of virulence regulators and indirectly by providing...... signalling to short-circuit host cell processes. Common to both intra- and extracellular proteases is the tight control of their proteolytic activities. In general, substrate recognition by the intracellular proteases is highly selective which is, in part, attributed to the chaperone activity associated...

  2. les ethmoidites aigues exteriorisees de l'enfant etude de 11 ...

    African Journals Online (AJOL)

    16 juin 2006 ... Acute ethmoiditis are bacterial infections of ethmoid sinuses, which may spread to the orbital or the endocranial spaces.They are the most frequent among bacterial sinusitis before 3 years. Population and methods:Our study is retrospective, it included all children hospitalized in our pediatric unit from 2000.

  3. Experimental studies of X-UV rays by a laser plasma: X-UV strioscopy by means of multilayer mirrors; Etude experimentale de la refraction X-UV par un plasma laser: strioscopie X-UV a l`aide de miroirs multicouches

    Energy Technology Data Exchange (ETDEWEB)

    Lutrin, F


    This thesis studies a new instrument -from its conception to the measures interpretation- that analyses electronic density gradient in the super critical transportation area of a laser plasma (0,35 {mu}m). This device, so-called of X-UV Schlieren, is based on the refraction property of a probe beam by an index gradient. Its specificity is the use of the X-UV emission at 13 nm (92 eV) of another laser plasma as X-UV probe. The conception and characterization of this instrument are defined thanks to both the emissivity and reflectivity properties of laser plasmas and the reflectivity properties of multilayers. Within this report are presented strioscopy images, spatially and spectrally resolved of an aluminium plasma from a 3.10{sup 12} W/cm{sup 2} laser flux, probed by a 13 nm wavelength. The device has to be closely aligned so as to obtain good contrast and good spatial resolution. For the first time, the refraction of a X-UV probe beam by a laser plasma is displayed. The experiments show that this refraction is all the more obvious for a gold probe plasma of energy 105 J and an aluminium probed plasma of energy 1 J. According to our plasma hydrodynamic simulation, the detected refraction corresponds to an electronic density gradient of 6,5.10{sup 25} electrons/cm{sup 4} in the two first microns of the sur-critical area. To study the parameters dependence of this gradient in the sur-critical area, several solutions for improving the instrument are produced. (author) 168 refs.

  4. Experimental and theoretical studies of the VUV emission and absorption spectra of H{sub 2}, HD and D{sub 2} molecules; Etude experimentale et theorique des spectres d'emission et d'absorption VUV des molecules H{sub 2}, D{sub 2} et HD

    Energy Technology Data Exchange (ETDEWEB)

    Roudjane, M


    The aim of this thesis is to carry out an experimental study of the absorption and emission spectra of the D{sub 2} and HD isotopes, with high resolution, in the VUV domain and to supplement it by a theoretical study of the excited electronic states involved in the observed transitions. The emission spectra of HD and D{sub 2} are produced by Penning discharge source operating under low pressure and are recorded in the spectral range 78 - 170 nm. The recorded spectra contains more than 20.000 lines. The analysis of the spectrum consists in identifying and assigning the lines to the electronic transitions between energy levels of the molecule. The present analysis is based on our theoretical calculations of the ro-vibrational energy levels of the excited electronic states and the transition probabilities from these states towards the energy levels of the fundamental state. The theoretical results are obtained by resolving the coupled equations between the excited electronic states B{sup 1}{sigma}{sub u}{sup 1}, B'{sup 1}{sigma}{sub u}{sup 1}, C{sup 1}{pi}{sub u}{sup 1} and D{sup 1}{pi}{sub u}{sup 1}, taking into account the nonadiabatic couplings between these states, and they are obtained in the adiabatic approximation for the excited electronic states B''B-bar{sup 1}{sigma}{sub u}{sup +}, D'{sup 1}{pi}{sub u}{sup 1} and D''{sup 1}{pi}{sub u}{sup 1}. The equations are resolved using a modern method based on the discretization variables representation method. In addition, we have carried out a study of the absorption spectra of the HD and D{sub 2} molecules.

  5. Experimental and numerical studies of the effect of plasticity mechanisms on the brittle rupture by cleavage in low alloy steels; Etudes experimentale et numerique de l'effet des mecanismes de plasticite sur la rupture fragile par clivage dans les aciers faiblement allies

    Energy Technology Data Exchange (ETDEWEB)

    Libert, M


    It is indispensable to guarantee the integrity of PWR reactor vessels during an accidental running: in this context, the understanding and the modelling of the mechanisms of brittle rupture of steels are decisive elements of the complicated estimation of the service life of reactor vessels. The models of local approach of rupture by cleavage are one of the main tools of anticipation of the tensile strength of low alloy steels. In this work, the effect of the stresses heterogeneities in a local criteria of initiation of cleavage has been taken into account. The results of the microstructure calculations are used for proposing a statistical description of the local stresses distribution evolution. This statistical approach allows to propose a local approach model of the rupture depending both of the mechanical heterogeneities and of the sizes distributions of the defects. The behaviour of the material and its evolution are characterized at the microscopic and macroscopic scales in the temperature range [25 C, -196 C]. Simple tensile tests, velocity and temperature rises tests and toughness tests have been carried out. A micro-mechanical behaviour model describing the plastic behaviour below the transition temperature T{sub a} has been proposed. The behaviour law is based on the deformation mechanisms described in bibliography and identified by an inverse method from mechanical tests. The TEM observations and the characterization of the behaviour thermally activated allow to determine several parameters of the model. Simulations are carried out in order to model the main stress distributions {sigma}{sub 1} in two bainite microstructures corresponding to the elementary volume of the local approach of the rupture. The temperature and the triaxiality effects on the evolutions of the heterogeneities is characterized. A distribution function describing the distribution of the local values of {sigma}{sub 1} in terms of the main and equivalent mean stresses {sigma}{sub 1} and {sigma}{sub m} in the microstructure is proposed. This function is used for formulating a model of rupture local approach integrating the distribution of the critical defects sizes and the distributions of {sigma}{sub 1}. It is shown that in some cases, the dispersion of the local stresses is sufficient to explain the dispersions of the rupture stresses at the scale of the elementary volume. The dispersions of the rupture stresses are in agreement with those given by the Beremin model. The taken into account of the mechanical heterogeneities allows to introduce a dependency of the rupture probability in terms of temperature, of deformation and of triaxiality. (O.M.)

  6. Experimental and theoretical study of bragg-Fresnel optics etched on multilayer structures. Application: lenses for X-Ray imaging; Etude experimentale et theorique d`optiques de bragg-Fresnel gravees sur miroirs interferentiels multicouches. Application: lentilles pour l`imagerie X

    Energy Technology Data Exchange (ETDEWEB)

    Soullie, G.


    This work concerns the study of a new type of X-ray focusing optics known as Bragg-Fresnel lenses developed for imaging in the X and X-UV range. These optics, etched on multilayer structure, combine the focusing properties of zone plate with the Bragg reflection of multilayer used like support. Using synchrotron sources and a plasma source produced by a laser, we tested the efficiency and the spatial resolution of these lenses. With a monochromatic beam, we first obtained the image of a object by using the first order diffraction of an elliptical off-axis Bragg-Fresnel lens. By using only one part of a lens, the superposition of different diffraction orders in focal plane can be avoided, thus improving the image contrast. In order to evaluate the chromatic aberrations of these lenses, we have summed on the same image, three exposures at different energies in the band pass of the multilayer. To reduce these kind of aberrations, we used a system composed of two off-axis lenses. To simplify the alignment, we tested an elliptical off-axis lens associated with a lamellar grating. Thus we are able to validate the theoretical approximation of an off-axis Bragg-Fresnel lens to a variable spaced grating. Finally, to show the perturbation brought by the zeroth order, we successively imaged a laser plasma source with a centred and an off-axis elliptical lenses. As with the synchrotron source, a set of images of a test object enabled us to improve the spatial resolution. (author).

  7. Self-ignition and oxidation of various hydrocarbons between 600 and 1000 K at high pressure: experimental study with fast compression machine and modeling; Autoinflammation et oxydation de divers hydrocarbures entre 600 et 1000 K a haute pression: etude experimentale en machine a compression rapide et modelisation

    Energy Technology Data Exchange (ETDEWEB)

    Ribaucour, M.


    Low- and intermediate-temperature oxidation and self-ignition of hydrocarbons play a major role in spark ignition, diesel and HCCI (homogenous charge compression ignition) engines. A deep understanding of the chemistry linked with both phenomena is necessary to improve the engines efficiency and to reduce the formation of pollutants. This document treats of works about the self-ignition and oxidation at high pressure of various hydrocarbons between 600 and 1000 deg. K. The experimental tool used is a fast compression machine fitted with a fast sampling system for the measurement of self-ignition delays and of the concentrations of intermediate oxidation products. The advantages and limitations of this tool are discussed. The self-ignition of various hydrocarbons is compared using pre-defined data which characterize the phenomenologies like cold flames, negative temperature coefficients and self-ignition limits. The hydrocarbons considered are pure or binary mixtures of alkanes, pent-1-ene and n-butyl-benzene. The development of high pressure oxidation reaction schemes of alkanes between 600 and 1000 deg. K is described. It is directly based on the analysis of intermediate oxidation products. This methodology is also applied to pent-1-ene and n-butyl-benzene. The construction of detailed thermo-kinetic models of oxidation and the modeling of phenomena are made for n-butane, n-heptane, for the 3 pentane isomers, for pent-1-ene and n-butyl-benzene. Finally, the perspectives of future works are evoked. They concern new modeling and new methodologies to be applied in more predictive thermo-kinetic models and the reduction of detailed models in order to include them inside fluid dynamics codes. (J.S.)

  8. Experimental study of high current negative ion sources D{sup -} / H{sup -}. Analysis based on the simulation of the negative ion transport in the plasma source; Etude experimentale de sources a fort courant d`ions negatifs D{sup -} / H{sup -}. Analyse fondee sur la simulation du transport des ions dans le plasma de la source

    Energy Technology Data Exchange (ETDEWEB)

    Riz, D.


    In the frame of the development of a neutral beam injection system able to work the ITER tokamak (International Thermonuclear Experimental Reactor), two negative ion sources, Dragon and Kamaboko, have been installed on the MANTIS test bed in Cadarache, and studies in order to extract 20 mA/cm{sup 2} of D{sup -}. The two production modes of negative ions have been investigated: volume production; surface production after cesium injection in the discharge. Experiments have shown that cesium seeding is necessary in order to reach the requested performances for ITER. 20 mA/cm{sup 2} have been extracted from the Kamaboko source for an arc power density of 2.5 kW/liter. Simultaneously, a code called NIETZSCHE has been developed to simulate the negative ions transport in the source plasma, from their birth place to the extraction holes. The ion trajectory is calculated by numerically solving the 3D motion equation, while the atomic processes of destruction, of elastic collisions H{sup -}/H{sup +} and of charge exchange H{sup -}/H{sup 0} are handled at each time step by a Monte Carlo procedure. The code allows to obtain the extraction probability of a negative ion produced at a given location. The calculations performed with NIETZSCHE have allowed to explain several phenomena observed on negative ion sources, such as the isotopic effect H{sup -}/D{sup -} and the influence of the polarisation of the plasma grid and of the magnetic filter on the negative ions current. The code has also shown that, in the type of sources contemplated for ITER, working with large arc power densities (> 1 kW/liter), only negative ions produced in volume at a distance lower that 2 cm from the plasma grid and those produced at the grid surface have a chance of being extracted. (author). 122 refs.

  9. Experimental study on the influence of charge exchange on the stopping power in the interaction of chlorine with a gas and a deuterium plasma; Etude experimentale de l`influence des echanges de charges sur le pouvoir d`arret dans l`interaction d`ions chlore avec un gaz et un plasma de deuterium

    Energy Technology Data Exchange (ETDEWEB)

    Nectoux, Marie [Inst. de Physique Nucleaire, Paris-11 Univ., 91 - Orsay (France)


    This thesis is placed in the context of the physics of energy deposition of a multicharged heavy ion beam in matter at intermediate energies. The experiment gave measurements of energy loss as a function of final charge state for chlorine ions at 1.7 MeV/u in deuterium gas or plasma. In this way, we explore the influence of charge state evolution, depending on experimentally measured capture and ionization cross sections and the electron density of the target, on energy loss. The target is cylindrical and enclosed by two fast valves. The plasma is created in the gas by a discharge, which induces a magnetic perturbation of the beam (lens effect). This effect induces a divergent and misaligned outgoing beam. A simulation including charge state and velocity evolution of the projectile in flight in the magnetic field has been made in order to optimize beam analysis, to reach a precision better than 10{sup -3} in energy measurement. This study led to removal of the target to the `Split Pole`, a refocusing magnetic spectrometer. The first results obtained clearly show the dependence of energy loss on exit charge and especially on its evolution in the target. This is explained in terms of the lengths covered by the projectile in its successive charge states in the target, which depends on target electron density and the medium considered. In plasma, we observed an energy distribution with exit charge twice that observed in gas, because of a strong decrease of charge exchange. A comparison of data obtained in gas with stopping power calculated from Bethe-Bloch-Barkas theory leads to the necessity of including spatial extension of the projectile charge in the theory. (author) 81 refs., 62 figs., 5tabs.

  10. Experimental study and modelling of high temperature creep flow and damage behaviour of 9Cr1Mo-NbV steel weldments; Etude experimentale et modelisation, du comportement, de l'endommagement et de la rupture en fluage a haute temperature de joint soudes en acier 9Cr1Mo-NbV

    Energy Technology Data Exchange (ETDEWEB)

    Gaffard, V


    Chromium martensitic stainless steels are under development since the 70's with the prospect of using them as structural components in thermal and nuclear power plants. The modified 9Cr1Mo-NbV steel is already used, especially in England and Japan, as a material for structural components in thermal power plants where welding is a commonly used joining technique. New generations of chromium martensitic stainless steels with improved mechanical properties for high pressure and temperature use are currently under development. However, observations of several in-service premature failures of welded components in 9Cr1Mo-NbV steel, outline a strong need for understanding the high temperature creep flow and damage behaviour of 9Cr1Mo-NbV steels and weldments. The present study aimed at experimentally determining and then modelling the high temperature creep flow and damage behaviour of both 9Cr1Mo-NbV steels and weldments (typically in the temperature range from 450 C to 650 C). The base metal was first studied as the reference material. It was especially evidenced that tempered chromium martensitic steels exhibit a change in both creep flow and damage behaviour for long term creep exposure. As a consequence, the classically performed extrapolation of 1,000 hours creep data to 100,000 hours creep lifetime predictions might be very hazardous. Based on experimental observations, a new model, integrating and coupling multiple creep flow and damage mechanisms, was developed in the framework of the mechanics of porous media. It was then successfully used to represent creep flow and damage behaviour of the base metal from high to low stress levels even for complex multiaxial loading conditions. Although the high temperature creep properties of the base metal are quite good, the occurrence of premature failure in weldments in high temperature creep conditions largely focused the attention of the scientific community. The lower creep strength of the weld component was also experimentally confirmed in the present study. The evaluation of creep flow and damage behaviour of the weldment requires two types of investigations, namely, the determination of the effect of welding on creep strength of the material especially in the heat affected zone (HAZ), and the investigation of the effect of the mismatch in creep properties between the weld metal, the base metal and the HAZ. To do so, the microstructure of the weakest HAZ was first reproduced on bulk specimens. These specimens were used to experimentally investigate the creep flow and damage behaviour of the weakest HAZ microstructure. The results of experiments allowed to fit a creep model for the simulated HAZ derived from the one designed for the base metal. The integration of constitutive equations of base metal, weld metal and simulated HAZ in finite element calculations of the heterogeneous weldment allows to study the effect of mismatch on creep properties and finally to predict creep lifetime of 9Cr1Mo-NbV welded components. (author)

  11. Experimental study and modelling of competitive adsorption equilibria of aromatics in liquid phase on X and Y faujasites; Etude experimentale et modelisation des equilibres d'adsorption competitive d'aromatiques en phase liquide sur des faujasites X et Y

    Energy Technology Data Exchange (ETDEWEB)

    Tournier, H.


    The separation of p-xylene from C{sub 8} aromatics is performed industrially by selective adsorption on zeolitic molecular sieves. The aim of this work is to study and model adsorption equilibria of C{sub 8} and C{sub 10} aromatics on X and Y zeolites. The experimental data are obtained by an entirely automated equipment allowing to work in a large range of temperature (50 deg. C - 250 deg. C). With this equipment, we can follow the evolution of the composition of the liquid phase and determine the composition of the adsorbed phase at equilibrium by a mass balance calculation and with an inert component. Two analytical techniques are used to determine the composition of the liquid phase: (1) a classical method using a gas chromatograph (GC) allowed to measure selectivities in the concentration range (3%-97%) in a component; (2) an original method based on the use and on the measure of {sup 13}C labelled xylenes was developed to investigate the ranges of strongly contrasting concentrations [0-3%] and [97%-100%] in a component, which are representative of high purity domains. Lastly, three thermodynamic models are used to describe the adsorption equilibria: the Langmuir-Freundlich model, the quasi-chemical model and the statistical model. The last model is the more interesting, because it is based on physical considerations. A new statistical model has been developed with taking into account some observations coming from adsorption phenomenon in zeolites. (author)

  12. Temperature effect on the poro-mechanical or hydraulic behaviour of a carbonated rock and a mortar: experimental studies; Influence de la temperature sur le comportement poromecanique ou hydraulique d'une roche carbonatee et d'un mortier: etudes experimentales

    Energy Technology Data Exchange (ETDEWEB)

    Lion, M


    The main objective of this study is to evaluate the temperature effect on the hydraulic and poro-mechanical behaviour of a limestone. Many experimental tests (porosity and permeability measurements, uniaxial and hydrostatic compressions tests) were carried out in order to study the thermal treatments effect and so the thermal microcracking effect on rock behaviour. Moreover, an experimental device for permeability measurements under high temperatures (until 200 C) was realized. This experimental device permitted to study the permeability variation of the limestone under thermal stresses. Finally, the behaviour of cementitious materials was studied; the temperature effect on the permeability of a mortar was examined. (author)

  13. Study of secondary electron emission from thin carbon targets with swift charged particles: heavy ions, hydrogen ions; Etude experimentale de l`emission electronique secondaire de cibles minces de carbone sous l`impact de projectiles rapides: ions lourds, ions hydrogene (atomiques, moleculaires ou sous forme d`agregats)

    Energy Technology Data Exchange (ETDEWEB)

    Billebaud, A.


    The main subject of this work is the study of electron emission from the two surfaces of thin solid targets bombarded with swift charged particles. The slowing down of swift ions in matter is mainly due to inelastic interaction with target electrons (ionization, excitation): the energy transfer to target electrons is responsible for the secondary electron emission process. The phenomenological and theoretical descriptions of this phenomena are the subject of the first chapter. We focused on secondary electron emission induced by different kind of projectiles on thin carbon foils. In chapter two we describe hydrogen cluster induced electron emission measurement between 40 and 120 keV/proton. These projectiles, composed of several atoms, allowed us to study and highlight collective effects of the electron emission process. We extended our study of electron emission to molecular (H{sub 2}{sup +}, H{sub 3}{sup +}) and composite (H{sup -}, H{sup 0}) projectiles at higher energies (<= 2 MeV): we have designed an experimental set-up devoted to electron emission statistics measurements which allowed us to study, among others things, the role of projectile electrons in secondary electron emission. This experiment is described in the third chapter. Finally, the fourth chapter describes new measurements of electron emission induced by energetic (13 MeV/u) and highly charged argon ion provided by the medium energy beam line (SME) of GANIL (Caen), which have been analyzed in the framework of a semi-empirical model of secondary electron emission. This set of experiments brings new results on composite projectile interaction with matter, and on the consequences of high energy deposition in solids. (author).

  14. Experimental study and modelling of physico-chemical mechanisms of clay-concrete interactions in the radioactive waste geological disposal context; Etude experimentale et modelisation des mecanismes physico-chimiques des interactions beton-argile dans le contexte du stockage geologique des dechetsradioactifs

    Energy Technology Data Exchange (ETDEWEB)

    Dauzeres, A.


    These research works are carried out as part of the radioactive wastes geological disposal feasibility study. The current option developed by Andra, includes several cementitious materials in contact with the surrounding Callovo-Oxfordian (COX) (an argillite). Concretes and argillite present very different pore solutions (ionic concentrations and pH). Controlled by the concentrations differences, the aqueous species diffusion in the solids generates chemical and physical disturbances. This study is based on experimental, analytical and numerical works, in order to identify the mechanisms controlling the clayey environment influence on cementitious materials. (author)

  15. Laser shocks on helium, hydrogen and diamond: an experimental study of the warm dense matter zone; Chocs laser sur l'helium, l'hydrogene et le diamant: une etude experimentale de la 'Warm Dense Matter'

    Energy Technology Data Exchange (ETDEWEB)

    Brygoo, St


    The purpose of this work was to develop a new approach of laser shocks on pre-compressed targets in order to collect data concerning the equation of state in the warm dense matter zone of the phase diagram. The accuracy of the measurement has been increased by the use of a new metrology based on quartz. Quartz is considered as a standard for the measurement of both the pressure and the density, a model of an isentropic relaxation based on a Grueneisen type approximation has been developed. By combining laser shocks with diamond anvil cells and by using this new metrology, we have investigated the following systems: diamond, helium, hydrogen, deuterium and hydrogen-helium mixtures. The results for helium agree very well with the predictions of the Saumon-Chabrier model. The results for deuterium are consistent with the latest results found in literature. As for the results concerning hydrogen, they have showed the limits of the quartz-based metrology. In fact, by being so little dense we are at the limit of the application range of the quartz relaxation. A mixture of helium-hydrogen (50 %) has been investigated, no sign of phase separation has been found.

  16. A micro-mechanical analysis and an experimental characterisation of the behavior and the damaging processes of a 16MND5 pressure vessel steel at low temperature; Etude micromecanique et caracterisation experimentale du comportement et de l'endommagement de l'acier de cuve 16MND5 a basses temperatures

    Energy Technology Data Exchange (ETDEWEB)

    Pesci, R


    As part of an important experimental and numerical research program launched by Electricite De France on the 16MND5 pressure vessel steel, sequenced and in-situ tensile tests are realized at low temperatures [-196 C;-60 C]. They enable to associate the observation of specimens, the complete cartography of which has been made with a scanning electron microscope (damaging processes, initiation and propagation of microcracks), with the stress states determined by X-ray diffraction, in order to establish relevant criteria. All these measurements enable to supply a two-scale polycrystalline modeling of behavior and damage (Mori-Tanaka/self-consistent) which is developed concurrently with the experimental characterization. This model proves to be a very efficient one, since it correctly reproduces the influence of temperature experimentally defined: the stress state in ferrite remains less important than in bainite (the difference never exceeds 150 MPa), whereas it is much higher in cementite. The heterogeneity of strains and stresses for each crystallographic orientation is well rendered; so is cleavage fracture normal to the {l_brace}100{r_brace} planes in ferrite (planes identified by electron back scattered diffraction during an in-situ tensile test at -150 C), which occurs sooner when temperature decreases, for a constant stress of about 700 MPa in this phase. (author)

  17. Experimental study and mechanical modelling of the effects of water-driving in an oil saturated chalk. Application to the petroleum industry; Etude experimentale et modelisation mecanique des effets du balayage a l'eau dans une craie saturee d'huile. Application a l'industrie petroliere

    Energy Technology Data Exchange (ETDEWEB)

    Mata, C.


    The production of hydrocarbons may sometimes lead to compaction of the oil-reservoir layers with substantial collapse of the soil surface; this mechanism is usually mentioned as Subsidence. Classical remedy to this problem is to sustain the fluid pressure with the help of water injection. However, in presence of high porosity chalk (porosity > 30%), this technique does not give effective results: the subsidence rate at North Sea reservoirs (Ekofisk field), after injection, has remained unchanged. Some justify the subsistence in the chalk as caused by phenomena of dissolution of the carbonates; others think it is connected to the destruction of capillary menisci. In this research, we follow this second approach. This study is finalized to a better understanding of the compaction mechanisms in pure carbonates when water is injected. This is done by proposing -in micro-scale first and in macro-scale after- a physical model that agrees with empirical observations. In order to meet this target, IFP skills and experience in physical and chemical measurements (taken before, after and during mechanical tests) have proficiently joined ENPC laboratory (CERMMO) knowledge of micro and macro modelling. The soft rock studied is a Paris basin's white chalk (Craie de Guerville). Its physical and chemical characterisation (i.e.: silica content, porosity, permeability) is very similar to North Sea chalk, but Guerville chalk has never been in contact with oil and generally it crops out of the ground surface. Strength parameters of this rock have been first determined by standard triaxial tests. Three different saturation configurations have been examined: dry, fully oil-saturated and fully water-saturated. As literature clearly state, dry chalk is stronger than oil saturated chalk and the first two are largely stronger than water saturated chalk. Therefore uniaxial strain water injection tests have been performed on oil saturated samples. Results, coupled with previous physical measurements and SEM observation, let us highlight two different contributions to mechanical strength of chalk-: 1) The cohesion due to solid micro-bridges between calcite grains (always present in intact samples); 2) The cohesion due to water capillary menisci (which decreases when the degree of water saturation increases). On these grounds, an elasto-plastic model is proposed is order to explain how contributions are coupled. In this modelling, plastic strain is an hardening parameter while water saturation ratio is a softening one. Applications are developed in order to relate water-driving, stress state and subsidence rate in an oil reservoir configuration. (author)

  18. Experimental study of hot electrons propagation and energy deposition in solid or laser-shock compressed targets: applications to fast igniter; Etude experimentale de la propagation et du depot d'energie d'electrons rapides dans une cible solide ou comprimee par choc laser: application a l'allumeur rapide

    Energy Technology Data Exchange (ETDEWEB)

    Pisani, F


    In the fast igniter scheme, a recent approach proposed for the inertial confinement fusion, the idea is to dissociate the fuel ignition phase from its compression. The ignition phase would be then achieved by means of an external energy source: a fast electron beam generated by the interaction with an ultra-intense laser. The main goal of this work is to study the mechanisms of the hot electron energy transfer to the compressed fuel. We intent in particular to study the role of the electric and collisional effects involved in the hot electron propagation in a medium with properties similar to the compressed fuel. We carried out two experiments, one at the Vulcan laser facility (England) and the second one at the new LULI 100 TW laser (France). During the first experiment, we obtained the first results on the hot electron propagation in a dense and hot plasma. The innovating aspect of this work was in particular the use of the laser-shock technique to generate high pressures, allowing the strongly correlated and degenerated plasma to be created. The role of the electric and magnetic effects due to the space charge associated with the fast electron beam has been investigated in the second experiment. Here we studied the propagation in materials with different electrical characteristics: an insulator and a conductor. The analysis of the results showed that only by taking into account simultaneously the two propagation mechanisms (collisions and electric effects) a correct treatment of the energy deposition is possible. We also showed the importance of taking into account the induced modifications due to the electrons beam crossing the target, especially the induced heating. (author)

  19. New experimental method to study the collective modes in exotic nuclei; influence of the superfluidity on the cooling time of a neutron star; Nouvelle methode experimentale dediee a l'etude des modes collectifs dans les noyaux exotiques; influence de la superfluidite sur le temps de refroidissement d'une etoile a neutrons

    Energy Technology Data Exchange (ETDEWEB)

    Monrozeau, Ch


    Giant monopole (GMR) and quadrupole (GQR) resonances have been measured in the {sup 56}Ni using inelastic scattering of 50 A.MeV deuteron at the Grand Accelerateur National d'Ions Lourds facility. This is the first experimental observation of isoscalar collective modes in a short-lived nucleus. The secondary beam was impinged on the active target Maya filled with a pure deuterium gas. Recoiling deuterons were detected in Maya and in a wall of nine silicon detectors. The GMR and GQR are centered at 19.3(0.5) and 16.2(0.5) MeV, respectively. Corresponding angular distributions were extracted from 3 to 7 degrees in the centre of mass frame. DWBA analysis based on RPA transition densities yields the percentage of the energy weighted sum rule exhausted: 136(27) % for the GMR et 76(13) % for the GQR. A finite temperature Hartree-Fock-Bogoliubov model was implemented to describe the 10 Wigner-Seitz cells which compose the inner crust of neutron stars and to microscopically calculate their specific heat. Calculations are performed with two contact pairing forces chosen to simulate the pairing properties of uniform neutron matter corresponding to the BCS approximation and to polarisation effects. Under the assumption of a rapid cooling of the core and an initial temperature of 100 keV in the inner crust, the cooling time of the star was estimated at 9 and 34 years, respectively. (author)

  20. Contribution to the study of copper and copper-arsenic archaeo-metallurgy using light element analysis and experimental fusion; Contribution a l`etude de la paleometallurgie du cuivre et du cuivre-arsenic a partir de l`analyse des elements legers et de fusions experimentales

    Energy Technology Data Exchange (ETDEWEB)

    Papillon, F


    The objective of this study is to try a direct reconstruction from ancient artefacts of the elaboration technology used in the dawning copper metallurgy. This word is based on both the light elements analysis and the carry out of the principles of physical metallurgy. However the study of an archaeological artefact necessitates the use of non destructive methods. A main aspect of this work consists in developing the most adequate metallographic technique and the methods for the determination of oxygen and carbon by ion beam analysis. Additionally experimental melting of copper and copper arsenic alloys were carried out in laboratory, under various temperature and atmosphere conditions, and `on the field` in Archeodrome de Beaune, in order to reconstruct part of the prehistorical craftsmanship. The results of measurement are consistent with our general knowledge of oxido-reduction phenomena and the behaviour of copper and copper arsenic alloys s in agreement with the prediction of thermodynamics. The nuclear analysis of three ancient artefacts showed that the oxygen and carbon contents were closer to those of the Archeodrome than those of the laboratory. Further studies of the field should consider all parameters controlling the physical-chemistry of charcoal fire. (author) 96 refs.

  1. Experimental study of chemical-mechanical coupling during percolation of reactive fluid through rocks under stress, in the context of the CO{sub 2} geological sequestration; Etude experimentale du couplage chimie-mecanique lors de la percolation d'un fluide reactif dans des roches sous contrainte, dans le contexte de la sequestration geologique du CO{sub 2}

    Energy Technology Data Exchange (ETDEWEB)

    Le Guen, Y


    CO{sub 2} injection into geological repositories will induce chemical and mechanical instabilities. The study of these instabilities is based on experimental deformation of natural rock samples under stress, in the presence of fluids containing, or not, dissolved CO{sub 2}. Triaxial cells used for the experiments permitted an independent control and measurement of stress, temperature, fluid pressure and composition. Vertical strains were measured during several months, with a resolution of 1.10{sup -12} s{sup -1} on the strain rate. Simultaneously, fluids were analysed in order to quantify fluid-rock interactions. For limestone samples, percolation of CO{sub 2}-rich fluids increases strain rate by a factor 1.7 up to 5; on the other hand, sandstone deformation remained almost the same. Increase in strain rate with limestone samples was explained by injected water acidification by the CO{sub 2} which increases rock solubility and reaction kinetics. On the opposite, small effect of CO{sub 2} on quartz explains the absence of deformation. X-ray observations confirmed the importance of rock composition and structure on the porosity evolution. Numerical simulations of rock elastic properties showed increasing shear stress into the sample. Measured deformation showed an evolution of reservoir rocks mechanical properties. It was interpreted as the consequence of pressure solution mechanisms both at grains contacts and on grain free surfaces. (author)

  2. Numerical and experimental study of the influence of the operational parameters on the formation mechanisms of oxides of nitrogen during the combustion of mixtures of cellulosic and plastic materials; Etude experimentale et numerique de l'influence des parametres operatoires sur les mecanismes de formation des oxydes d'azote lors de la combustion de melanges de materiaux cellulosiques et plastiques

    Energy Technology Data Exchange (ETDEWEB)

    Andzi Barhe, T.


    The current thesis was performed within a collaboration between the Laboratoire de Combustion et de Detonique (LCD of the University of Poitiers) and the Laboratoire de Physique et de Chimie d'Environnement (LPCE) of the University of Ouagadougou. It was financed by Agency for Environment and Energy Management (ADEME). The principle object of this study is the optimisation of the combustion process during the incineration of waste. This optimisation is aimed at the reduction of the polluting emissions, principally CO and NO, during the incineration of cellulosic and plastic materials. It involves the analysis of the influence of the operational parameters on the polluting emissions and the control of reaction mechanisms of formation and reduction of these pollutants during the combustion process. Consequently, the study was performed in two parts: an experimental part and a numerical part. The experimental part was realised using a fixed bed counterflow reactor. This setup simulates the combustion within an industrial waste incinerator. The reactor allows the combustion of a vertical layer of waste mixture (wood, cardboard, PET, polyamide) to be followed. Three model mixtures representative of the makeup of household waste were studied in order to determine the influence of the composition of the waste on the emission of pollutants (CO and NO). The obtained results show that this parameter has a practically negligible influence within the tested parameter range. Consequently the formation of pollutants depends on the operating parameters - the equivalence ratio and the temperature. A numerical study of the influence of these parameters in order to show their impact on the mechanisms of pollutant formation and to determine the chemical mechanisms involved in the formation of oxides of nitrogen. The numerical study was performed with software developed at the LCD. This programme based on a detailed chemical model coupled to a simple physical model. It uses the calculation code CHEMKIN. It permits the simulation of combustion process within the gaseous zone of the fixed bed reactor. The programme is initialized with the results of the analysis of the pyrolysis which had previously been performed at the LCD on the materials which make up the model waste mixture. The pyrolysis products identified during this study are: HCN, NH{sub 3}, NO, NO{sub 2}, CO and light hydrocarbons. The results show that the simulation enables the determination of reactional mechanisms of the formation and reduction of oxides of nitrogen in the three combustion regimes identified during the experimental study. For each of the three regimes, a study of the impact of the combustion parameters on the yield of NO was performed. It can be seen that the response to the parameters is itself heavily dependent on the combustion regime. (author)

  3. Experimental study of Au+Au collisions at E/A=150 and 400 MeV: comparison with the GQMD-matrix model; Etude experimentale des collisions Au+Au a E/A=150 et 400 MeV: comparaison avec le modele matrice-GQMD

    Energy Technology Data Exchange (ETDEWEB)

    Ramillien, V.


    We present new experimental data obtained with the FOPI detector at the SIS Heavy ions accelerator (Darmstadt, Germany), for the Au + Au heavy ion collisions at 150 and 400 A.MeV incident energy. The sideward flow, determined form the method of Danielewicz without reaction plane reconstruction, and the nuclear stopping are studied as a function of the centrality of the collisions and as a function of the fragment charge. In order to study the nuclear in-medium effects, which act on the N-N cross-sections and potential and hence on experimental observables like the nuclear matter flow and stopping, these results are compared with the predictions of two different QMD versions. The first one (GQMD) offers a fully microscopic calculation of the cross-sections and potential in the G-matrix formalism and naturally includes the in-medium effects. This version is for the first time confronted to experiment. The second one (IQMD) uses a standard Skyrme potential plus a momentum dependent term in order to mimic the in-medium effects. We find that the experimental stopping is overestimated for 30-40% in the models IQMD and GQMD. Concerning the flow, we find that the momentum dependence of the potential in GQMD is somewhat stronger as can be seen for the more peripheral events and fits better the data in this case. On the other hand, the model GQMD clearly lacks of density dependence to reproduce the more central events. (author). 52 refs., 57 figs., 11 tabs.

  4. Experimental and modeling study of the impact of vertical transport processes from the boundary-layer on the variability and the budget of tropospheric ozone; Etude experimentale et numerique de l'influence des processus de transport depuis la couche-limite sur la variabilite et le bilan d'ozone tropospherique

    Energy Technology Data Exchange (ETDEWEB)

    Colette, A


    Closing the tropospheric ozone budget requires a better understanding of the role of transport processes from the major reservoirs: the planetary boundary layer and the stratosphere. Case studies lead to the identification of mechanisms involved as well as their efficiency. However, their global impact on the budget must be addressed on a climatological basis. This manuscript is thus divided in two parts. First, we present case studies based on ozone LIDAR measurements performed during the ESCOMPTE campaign. This work consists in a data analysis investigation by means of a hybrid - Lagrangian study involving: global meteorological analyses, Lagrangian particle dispersion computation, and mesoscale, chemistry - transport, and Lagrangian photochemistry modeling. Our aim is to document the amount of observed ozone variability related to transport processes and, when appropriate, to infer the role of tropospheric photochemical production. Second, we propose a climatological analysis of the respective impact of transport from the boundary-layer and from the tropopause region on the tropospheric ozone budget. A multivariate analysis is presented and compared to a trajectography approach. Once validated, this algorithm is applied to the whole database of ozone profiles collected above Europe during the past 30 years in order to discuss the seasonal, geographical and temporal variability of transport processes as well as their impact on the tropospheric ozone budget. The variability of turbulent mixing and its impact on the persistence of tropospheric layers will also be discussed. (author)

  5. Theoretical and experimental study of the double ionization by electron impact involving the Auger effect: processes and exchanges interferences; Etude theorique et experimentale de la double ionisation par impact electronique incluant l'effet auger: interferences d'echanges et de processus

    Energy Technology Data Exchange (ETDEWEB)

    Catoire, F


    In this work, double ionisation mechanisms of argon by electron impact in which the Auger effect is included have been studied as a function of the incident electron energy. Five and six fold differential cross sections in angle and in energy have been measured and analysed in a coplanar geometry. The efficiency of the apparatus has been improved by the use of a new toroidal analyser. For the first time, the six fold differential cross section in which the Auger electron and the ejected electron with identical kinetic energies (205 eV) are involved, was measured at an incident energy of 956 eV in the case of argon. In the theoretical models developed during this work, the triple continuum is represented by a manifold of coulomb waves describing the interaction of all electrons with the residual ion. Exchange effects between electrons were also included in the models. Comparison between experimental and theoretical results allows to study the relative contribution of the Auger process and the direct double ionisation on the angular dependence five fold differential cross section. In particular, the Auger process contribution seems to become increasingly important as the incident energy is increased.

  6. Theoretical and experimental study of high strain, high strain rate materials viscoplastic behaviour. Application to Mars 190 steel and tantalum; Etude theorique et experimentale du comportement viscoplastique des materiaux aux grandes deformations et grandes vitesses de deformations. Application a l'acier mars 190 et au tantale

    Energy Technology Data Exchange (ETDEWEB)

    Juanicotena, A


    This work enters in the general framework of the study and modelling of metallic materials viscoplastic behaviour in the area of high strain and high strain rate, from 10{sup 4} to 10{sup 5} s{sup -1}. We define a methodology allowing to describe the behaviour of armor steel Mars 190 and tantalum in the initial area. In a first time, the study of visco-plasticity physical mechanisms shows the necessity to take into account some fundamental processes of the plastic deformation. Then, the examination of various constitutive relations allows to select the Preston-Tonks-Wallace model, that notably reproduce the physical phenomenon of the flow stress saturation. In a second part, a mechanical characterization integrating loading direction, strain rate and temperature effects is conducted on the two materials. Moreover, these experimental results allow to calculate associated constants to Preston-Tonks-Wallace, Zerilli-Armstrong and Johnson-Cook models for each material. In a third time, in order to evaluate and to validate these constitutive laws, we conceive and develop an experimental device open to reach the area of study: the expanding spherical shell test. It concerns to impose a free radial expanding to a thin spherical shell by means a shock wave generated by an explosive. By the radial expanding velocity measure, we can determine stress, strain rate and strain applied on the spherical shell at each time. In a four and last part, we evaluate constitutive models out of their optimization area's. This validation is undertaken by comparisons 'experimental results/calculations' with the help of global experiences like expanding spherical shell test and Taylor test. (author)

  7. Experimental study and kinetic modeling of the thermal degradation of aromatic volatile organic compounds (benzene, toluene and xylene-para) in methane flames; Etude experimentale et modelisation cinetique de la degradation thermique des composes organiques volatils aromatiques benzenes, toluene et para-xylene dans des flammes de methane

    Energy Technology Data Exchange (ETDEWEB)

    Dupont, L.


    This study treats of the thermal degradation of a family of aromatic volatile organic compounds (VOCs) in laminar premixed methane flames at low pressure. The experimental influence of benzene, toluene and xylene-para on the structure of a reference methane flame has been studied. The molar fraction profiles of the stable and reactive, aliphatic, aromatic and cyclic species have been established by the coupling of the molecular beam sampling/mass spectroscopy technique with the gas chromatography/mass spectroscopy technique. Temperature profiles have been measured using a covered thermocouple. A detailed kinetic mechanism of oxidation of these compounds in flame conditions has been developed. Different available sub-mechanisms have been used as references: the GDF-Kin 1.0 model for the oxidation of methane and the models of Tan and Franck (1996) and of Lindstedt and Maurice (1996) in the case of benzene and toluene. In the case of para-xylene, a model has been developed because no mechanisms was available in the literature. These different mechanisms have been refined, completed or adjusted by comparing the experimental results with those obtained by kinetic modeling. The complete kinetic mechanism, comprising 156 chemical species involved in 1072 reactions allows to reproduce all the experimental observations in a satisfactory manner. The kinetic analysis of reactions velocity has permitted to determine oxidation kinetic schemes for benzene, toluene, xylene-para and for the cyclopentadienyl radical, main species at the origin of the rupture of the aromatic cycle. Reactions of recombination with the methyl radicals formed during methane oxidation, of the different aromatic or aliphatic radicals created during the oxidation of aromatics, play an important role and lead to the formation of several aromatic pollutants (ethyl-benzene for instance) or aliphatic pollutants (butadiene or penta-diene for instance) in flames. (J.S.)

  8. Experimental study of kinetic and mechanism of dissolution of apatite structured minerals. Application to the prediction of the long term behavior of an actinides storage host matrix; Etude experimentale de la cinetique et des mecanismes d'alteration de mineraux apatitiques. Application au comportement d'une ceramique de confinement d'actinides mineurs

    Energy Technology Data Exchange (ETDEWEB)

    Chairat, C


    The motivation for this study is to assess the potential of using apatite structured ceramics as long-lived actinide storage hosts. To assess their ability to resist aqueous corrosion, the dissolution of natural fluoro-apatite and synthetic Nd-britholite (neodymium is a proxy for the trivalent actinides) was studied. Mineral surfaces were characterized using a combined spectrometric, electrokinetic and potentiometric approach and dissolution rates were measured in closed and open system reactors as a function of solution composition. Experimental results suggest apatitic minerals dissolve via distinct step sequence: 1) fluoride release, 2) release of the calcium situated in the M1, and 3) the simultaneous removal of phosphate and calcium II via the breaking of only Ca-O bonds. TST based rate equations based in this mechanism accurately describe fluoro-apatite and synthetic britholite dissolution rates as a function of solution composition. Nd release rates are limited by precipitation of Nd-rhabdophane. (author)

  9. Adsorption of Cs{sup +}, Ni{sup 2+} and lanthanides onto a kaolinite and Na-montmorillonite up to 150{sup 0}C: an experimental and modeling study; Adsorption de Cs{sup +}, Ni{sup 2+} et des lanthanides sur une kaolinite et une smectite jusqu'a 150{sup 0}C: etude experimentale et modelisation

    Energy Technology Data Exchange (ETDEWEB)

    Tertre, E


    The motivation for this study is to assess the temperature effect on the clay minerals sorption properties. Sorption and desorption of Cs{sup +}, Ni{sup 2+} et Ln{sup 3+} onto a montmorillonite and a kaolinite were performed by batch experiments between 25 and 150 C, and in different pH and ionic strengths conditions. Sorption enthalpies varying between 0 and 80 kJ/mol were then calculated. For europium, surface spectroscopic analyses confirmed that the mechanism involved is adsorption, including at 150 C. Moreover, this method allowed us to obtain qualitatively the different adsorption equilibrium occurring during the reaction. An acid/base study of the clay surfaces was performed in order to assess the temperature effect on the surface charge of these minerals. Then, a surface complexation model including edge sites and structural sites was proposed to interpret the acid/base data and the europium sorption data. (author)

  10. Thermal stability of the aromatic fraction of Safaniya crude oil (Middle East): experimental study, kinetic scheme by molecular classes and geochemical implications; Stabilite thermique de la fraction aromatique de l'huile brute safaniya (Moyen-Orient): etude experimentale, schema cinetique par classes moleculaires et implications geochimiques

    Energy Technology Data Exchange (ETDEWEB)

    Al Darouich, T.


    The thermal evolution of reservoir oils is controlled by the kinetics of cracking reactions. The present work is concerned with the study of the thermal stability of the light aromatic components (C{sub 6}-C{sub 14}) of crude oils under geological conditions. The aim is to predict this stability through a mode l derived from laboratory pyrolyses. The light cut < 250 deg. C of Safaniya crude oil, corresponding to the C{sub 15} components, was obtained by fractionated distillation; pure aromatic fraction was then separated by liquid chromatography. Detailed molecular characterisation of the aromatic fraction was acquired using HPLC, GC and GC/MS. Then, quantified individual aromatic compounds were lumped into six molecular classes: BTXN, methyl-aromatics, alkyl-aromatics, naphthenic-aromatics, indene and sulphur - containing aromatics. Pyrolyses of the aromatic fraction were performed in gold tubes at 100 bars and different temperature/time conditions in a wide range (1 to 93%) of global conversion. Pyrolysis effluents were analysed and lumped into classes. The pyrolysis data were used to elaborate a semi - empirical kinetic scheme of 13 stoichiometric reactions for the primary and secondary cracking of the unstable classes. The scheme kinetic parameters were first estimated, and then numerically optimised, with the constraints of mass balance and hydrogen conservation. A set of pyrolysis experiments was performed at 375 deg.C under high pressures: 400, 800 and 1200 bars. Increasing slowing down in conversion rate with increasing pressure was thus observed compared to experiments at 100 bars. A slight selective effect of pressure on the different aromatic classes of the charge and on the product distribution was evidenced. The extrapolation of the kinetic model to the conditions of Elgin Field (North Sea) showed that pressure effect should shift the thermal cracking of light aromatics to higher temperatures by almost 8 deg. C. (author)

  11. Characterization of domestic wastes incineration clinkers. Experimental study of the dioxines impact on the environment. Program ''industrial wastes'' DRC 01; Caracterisation des machefers d'incineration d'ordures menageres. Etude experimentale de l'impact des dioxines sur l'environnement. Programme ''dechets industriels'' DRC 01

    Energy Technology Data Exchange (ETDEWEB)

    Brazillet, C.; Badreddine, R


    Clinkers resulting from domestic wastes incineration are used in road construction since May 1994. A first study realized by the INERIS showed the poor dioxines content in clinkers and their weak transfer in the environment. In order to verify the hypothesis presented in this study, an experimental study controlled different structures using clinkers. It showed a difference between the clinkers used in old structures which reveal a higher dioxines content and the clinkers used in more recent structures which present weak dioxines content. The use of clinkers, even in the case of significant dioxines level, has not a negative impact on the environment taking into account the transfer modes of these substances. (A.L.B.)

  12. Experimental studies and modeling of X-Rays multilayer mirrors damages under high X-Ray flux generated by a laser-plasma experiment; Etude experimentale et modelisation de l`endommagement des miroirs multicouches X soumis a de hauts flux de rayonnement X dans le cadre de l`experience plasma-laser

    Energy Technology Data Exchange (ETDEWEB)

    Le Guern, F


    We have been able with this work to point out characterize X-Rays multilayers mirrors damages. We have designed two experimental set-up which have been installed in the HELIOTROPE experimental chamber of the OCTAL facility located at the CEA in Limeil-Valenton. We have demonstrated that X-Rays multilayer mirrors properties were drastically modified by X-Rays emitted by a golden laser plasma. We have, more precisely, introduced the damage speed concept to quantify the expansion of the multilayer mirror period. We have been able to classify different multilayer mirrors in function of their resistance to damage and we have demonstrated that a silicate layer deposited on a mirror allowed to increase his resistance to damage. In a second part we have developed a simulation tool in order to simulate the X-Rays multilayer mirrors optical properties modifications. We have therefore coupled a thermo-mechanic code with an optical program. The results of the simulations are in a rather good agreement with the experiments and can be used to predict, before experiments, the multilayer mirror behavior under X-Rays irradiation. (author) 55 refs.

  13. Experimental and theoretical study of Bragg-Fresnel focalizing optical systems engraved on multi layers interferential mirrors adapted to X and X-UV fields; Etude experimentale et theorique d`optiques focalisantes de type Bragg-Fresnel gravees sur des miroirs interferentiels multicouches adaptes aux domaines X et X-UV

    Energy Technology Data Exchange (ETDEWEB)

    Idir, M.


    This work concerns the study of a particular type of X-ray focusing optics known as Bragg-Fresnel lenses, formed through ion-etching of multilayered structures. Using the Super-ACO (LURE/Orsay) synchrotron storage ring, we tested several Bragg-Fresnel lenses having either linear or elliptical geometries (producing a line or a point focus, respectively). Diffraction profiles were first obtained for the linear lenses ion-etched on W/Si multilayers of nano-metric period. The experimental results were compared with our theoretical predictions. We next proposed and tested a solution to the problem superposing the different diffraction orders in the focal plane, that of fabricating Bragg-Fresnel lenses with an off-axis configuration, first for the linear and then the elliptical geometry. An experimental application, for an off-axis elliptical lens produced a focused X-ray spot of 5 x 10 microns{sup 2} for the Super-ACO synchrotron source. The same lens also produced a 1/3-size X-ray image of a grid-like object at 1750 eV using the first and third diffraction orders. (author).

  14. Experimental study of the spin structure of the neutron ({sup 3}He) with low Q{sup 2}: a relationship between the Bjorken and Gerasimov-Drell-Hearn sum rules; Etude experimentale de la structure en spin du neutron ({sup 3}He) a bas Q{sup 2}: une connexion entre les regles de somme de Bjorken et Gerasimov-Drell-Hearn

    Energy Technology Data Exchange (ETDEWEB)

    Deur, A


    This thesis presents an experimental study of the neutron (and {sup 3}He) spin structure with a particular emphasis in the resonance domain (experiment E94010 that took place in 1997 at Jefferson Lab (TJNAF or formerly CEBAF) in Virginia). A polarized {sup 3}He target was built in order to achieve this study since polarized {sup 3}He nuclei can be seen as polarized neutrons. This target allowed the measurement of the polarized absolute cross sections {sigma}{sub 1/2}(Q{sup 2}, {nu}) and {sigma}{sub 3/2}(Q{sup 2}, {nu}) from the inclusive reaction {sup {sup {yields}}{sup 3}He}({sup {yields}}e, e')X for incident beam energies ranging from 0.86 GeV to 5.07 GeV at a scattering angle of 15.5 deg. The Q{sup 2} evolution of the generalized Gerasimov-Drell-Hearn (GDH) integral on {sup 3}He and on neutron was measured from 0.1 GeV{sup 2} to 1.0 GeV{sup 2} in order to understand the transition between perturbative QCD and non-perturbative QCD. The integration domain in {nu} (the energy loss of the scattered electron) is from the pion threshold to about 2.5 GeV which covers both the resonance region and the Deep Inelastic Scattering. The high precision of our data constrains the models giving the Q{sup 2} evolution of the generalized GDH integral. The polarized quasi-elastic scattering was also measured. The cross section {sigma}{sup TT}(Q{sup 2}, {nu}) on {sup 3}He and the spin structure functions g{sub 1}{sup {sup 3}He}(Q{sup 2}, {nu}) and g{sub 2}{sup {sup 3}He}(Q{sup 2}, {nu}) are presented. These data are an indication that the higher-twists are small in our kinematics domain and that the Bloom-Gilman duality seems to hold for the polarized spin structure functions. (author)

  15. Methodological study of the diffusion of interacting cations through clays. Application: experimental tests and simulation of coupled chemistry-diffusion transport of alkaline ions through a synthetical bentonite; Etude methodologique de la diffusion de cations interagissants dans les argiles. Application: mise en oeuvre experimentale et modelisation du couplage chimie-diffusion d'alcalins dans une bentonite synthetique

    Energy Technology Data Exchange (ETDEWEB)

    Melkior, Th


    The subject of this work deals with the project of underground disposal of radioactive wastes in deep geological formations. It concerns the study of the migration of radionuclides through clays. In these materials, the main transport mechanism is assumed to be diffusion under natural conditions. Therefore, some diffusion experiments are conducted. With interacting solutes which present a strong affinity for the material, the duration of these tests will be too long, for the range of concentrations of interest. An alternative is to determine on one hand the geochemical retention properties using batch tests and crushed rock samples and, on the other hand, to deduce the transport parameters from diffusion tests realised with a non-interacting tracer, tritiated water. These data are then used to simulate the migration of the reactive elements with a numerical code which can deal with coupled chemistry-diffusion equations. The validity of this approach is tested by comparing the numerical simulations with the results of diffusion experiments of cations through a clay. The subject is investigated in the case of the diffusion of cesium, lithium and sodium through a compacted sodium bentonite. The diffusion tests are realised with the through-diffusion method. The comparison between the experimental results and the simulations shows that the latter tends to under estimate the propagation of the considered species. The differences could be attributed to surface diffusion and to a decrease of the accessibility to the sites of fixation of the bentonite, from the conditions of clay suspensions in batch tests to the situation of compacted samples. The influence of the experimental apparatus used during the diffusion tests on the results of the measurement has also been tested. It showed that these apparatus have to be taken into consideration when the experimental data are interpreted. A specific model has been therefore developed with the numerical code CASTEM 2000. (author)

  16. Low temperature oxidation, co-oxidation and auto-ignition of olefinic and aromatic blending compounds: Experimental study of interactions during the oxidation of a surrogate fuel; Oxydation, co-oxydation et auto-inflammation a basses temperatures d'alcenes et aromatiques types: etude experimentale des interactions au sein d'un carburant-modele

    Energy Technology Data Exchange (ETDEWEB)

    Vanhove, G.


    The low-temperature (600-900 K) and high-pressure (5-25 bar) oxidation and auto-ignition of the three position isomers of hexene, of binary mixtures of 1-hexene, toluene and iso-octane, and of a surrogate fuel composed of these three compounds were studied in motor conditions using a rapid compression machine. Auto-ignition delay times were measured as long as intermediate products concentrations during the delay. The results show that the oxidation chemistry of the hexenes is very dependent on the position of the double bond inside the molecule, and that strong interactions between the oxidation mechanisms of hydrocarbons in mixtures can occur. The data obtained concerning the surrogate fuel give a good insight into the behaviour of a practical gasoline after an homogeneous charge compression. (author)

  17. Bacteriële meningitis

    NARCIS (Netherlands)

    Brouwer, M. C.; van de Beek, D.


    Bacterial meningitis is a severe disease which affects 35.000 Europeans each year and has a mortality rate of about 20%. During the past 25 years the epidemiology of bacterial meningitis has changed significantly due to the implementation of vaccination against Haemophilus influenzae, Neisseria

  18. Bacterial meningitis in immunocompromised patients

    NARCIS (Netherlands)

    van Veen, K.E.B.


    Bacterial meningitis is an acute infection of the meninges, in The Netherlands most commonly caused by Streptococcus pneumoniae and Neisseria meningitides. Risk factors for acquiring bacterial meningitis include a decreased function of the immune system. The aim of this thesis was to study

  19. Molecular approaches for bacterial azoreductases

    Directory of Open Access Journals (Sweden)

    Montira Leelakriangsak


    Full Text Available Azo dyes are the dominant types of synthetic dyes, widely used in textiles, foods, leather, printing, tattooing, cosmetics, and pharmaceutical industries. Many microorganisms are able to decolorize azo dyes, and there is increasing interest in biological waste treatment methods. Bacterial azoreductases can cleave azo linkages (-N=N- in azo dyes, forming aromatic amines. This review mainly focuses on employing molecular approaches, including gene manipulation and recombinant strains, to study bacterial azoreductases. The construction of the recombinant protein by cloning and the overexpression of azoreductase is described. The mechanisms and function of bacterial azoreductases can be studied by other molecular techniques discussed in this review, such as RT-PCR, southern blot analysis, western blot analysis, zymography, and muta-genesis in order to understand bacterial azoreductase properties, function and application. In addition, understanding the regulation of azoreductase gene expression will lead to the systematic use of gene manipulation in bacterial strains for new strategies in future waste remediation technologies.

  20. Contribution to the study of french pitchblendes; Contribution a l'etude des pechblendes francaises

    Energy Technology Data Exchange (ETDEWEB)

    Geffroy, J. [Commissariat a l' Energie Atomique, Lab. de Mineralogie, Centre de Chatillon (France). Centre d' Etudes Nucleaires; Sarcia, J.A. [Commissariat a l' Energie Atomique, Div. de la Crouzille, Haute Vienne (France). Centre d' Etudes Nucleaires


    The authors first review the characteristics of uraninite-pitchblende, as deduced of present literature. They set apart from typical pitchblende a black oxide aspect, which probably corresponds to neo-formations, and a 'para-pitchblende' aspect, which they relate to deep sur-oxidation of normal pitchblende. They insist on the easy replacement of pitchblende by silica. and give indications as to changes in vein stones (fluorite, quartz, etc...). A detailed study of paragenesis and successions in french uranium districts follows (including discussion of uranium of uranium-bearing coals). The authors attempt to classify french pitchblende veins. They are chiefly epithermal and poor in satellite ores. Three types of deposits are identified: massive - pitchblende type, silica type, fluorite type. These deposits, as those of Portugal, are included in granite, Central-European peri-batholitic types where uranium associates which Ni, Co, Bi and Ag, are in France both rare and poor. Finally, the authors attempt to bring out in the european Hercynian area a particular distribution of paragenetic types. (authors) [French] Les auteurs recapitulent d'abord les caracteres et les occurences de l'uraninite - pechblende, tels qu'ils peuvent etre degages de l'actuelle bibliographie. Ils exposent ensuite les faits qui du point de vue mineralogique seulement ressortent de l'etude mineralogique et chalcographique des pechblendes francaises et de leurs satellites. Ils distinguent de la pechblende-type un facies oxyde noir; correspondant probablement a une neoformation, et un facies parapechblende, qui est rapporte a une sur oxydation hypogene de la pechblende proprement dite. Ils insistent sur le facile remaniement de la pechblende par la slice; et donnent quelques precisions sur les modifications des gangues (fluorine, quartz, etc...). Suit l'etude detaillee des parageneses et des successions dans les districts uraniferes francais: Divisions du

  1. Zoonotic bacterial meningitis in human adults

    NARCIS (Netherlands)

    van Samkar, Anusha; Brouwer, Matthijs C.; van der Ende, Arie; van de Beek, Diederik


    To describe the epidemiology, etiology, clinical characteristics, treatment, outcome, and prevention of zoonotic bacterial meningitis in human adults. We identified 16 zoonotic bacteria causing meningitis in adults. Zoonotic bacterial meningitis is uncommon compared to bacterial meningitis caused by

  2. Evolution of Bacterial Suicide (United States)

    Tchernookov, Martin; Nemenman, Ilya


    While active, controlled cellular suicide (autolysis) in bacteria is commonly observed, it has been hard to argue that autolysis can be beneficial to an individual who commits it. We propose a theoretical model that predicts that bacterial autolysis is evolutionarily advantageous to an individualand would fixate in physically structured environments for stationary phase colonies. We perform spatially resolved agent-based simulations of the model, which predict that lower mixing in the environment results in fixation of a higher autolysis rate from a single mutated cell, regardless of the colony's genetic diversity. We argue that quorum sensing will fixate as well, even if initially rare, if it is coupled to controlling the autolysis rate. The model does not predict a strong additional competitive advantage for cells where autolysis is controlled by quorum sensing systems that distinguish self from nonself. These predictions are broadly supported by recent experimental results in B. subtilisand S. pneumoniae. Research partially supported by the James S McDonnell Foundation grant No. 220020321 and by HFSP grant No. RGY0084/2011.

  3. Electromagnetism of Bacterial Growth (United States)

    Ainiwaer, Ailiyasi


    There has been increasing concern from the public about personal health due to the significant rise in the daily use of electrical devices such as cell phones, radios, computers, GPS, video games and television. All of these devices create electromagnetic (EM) fields, which are simply magnetic and electric fields surrounding the appliances that simultaneously affect the human bio-system. Although these can affect the human system, obstacles can easily shield or weaken the electrical fields; however, magnetic fields cannot be weakened and can pass through walls, human bodies and most other objects. The present study was conducted to examine the possible effects of bacteria when exposed to magnetic fields. The results indicate that a strong causal relationship is not clear, since different magnetic fields affect the bacteria differently, with some causing an increase in bacterial cells, and others causing a decrease in the same cells. This phenomenon has yet to be explained, but the current study attempts to offer a mathematical explanation for this occurrence. The researchers added cultures to the magnetic fields to examine any effects to ion transportation. Researchers discovered ions such as potassium and sodium are affected by the magnetic field. A formula is presented in the analysis section to explain this effect.

  4. Bacterial growth on macrophyte leachate and fate of bacterial production

    International Nuclear Information System (INIS)

    Findlay, S.; Carlough, L.; Crocker, M.T.; Gill, H.K.; Meyer, J.L.; Smith, P.J.


    The role bacteria play in transferring organic carbon to other trophic levels in aquatic ecosystems depends on the efficiency with which they convert dissolved organic [ 14 C]-labelled carbon into bacterial biomass and on the ability of consumers to graze bacteria. The authors have measured the conversion efficiency for bacteria growing on macrophyte-derived dissolved organic carbon and estimated the amount of bacterial production removed by grazing. Bacteria converted this DOC into new tissue with an efficiency of 53%, substantially higher than the apparent conversion efficiency of macrophyte-derived particulate organic carbon or other types of DOC. Two estimates of grazing indicate that the decline in bacterial numbers after the bloom was probably due to grazing by flagellates. These results show the significance of the bacterial link between DOC and other trophic levels

  5. The study of neutron transport by oscillation method; Etude du transport des neutrons par la methode de modulation

    Energy Technology Data Exchange (ETDEWEB)

    Raievski, V


    The oscillation method is of very general use for studying the behavior of thermal neutrons in media. The main experiments are described and a general theory of them is given. This theory, which is presented in the first part, is established using the two-group approximation which has proved its efficiency in the case of thermal neutron piles. The validity of the two-group approximation is recalled. This allows definition of the meaning of the parameters used in the theory and which are measured in these experiments. The experiments carried out by this method are described, especially those performed at the Centre d'Etudes Nucleaires de Saclay where the method has been extensively used. These experiments are interpreted by means of the general theory given previously. In this way, the identity of parameters measured by this method and those given by the theory is proved. This is particularly conclusive is the case of the mean life of neutrons in a pile. (author) [French] La methode de modulation est un procede tres general d'etude des proprietes neutroniques des milieux contenant des neutrons thermiques. Le present rapport a pour but de decrire les principales de ces experiences et d'en donner une theorie generale. Cette theorie, exposee dans la premiere partie, est etablie dons le cadre de l'approximation a deux groupes de vitesse qui a prouve son efficacite dons le cas des piles a neutrons thermiques. Le domaine de validite de l'approximation a deux groupes est rappele au debut, ce qui permet de definir avec precision la signification des parametres qui entrent dons la theorie et qui font l'objet de ces mesures. La deuxieme partie decrit les experiences realisees, en particulier celles effectuees au Centre d'Etudes Nucleaires de Saclay ou la methode a ete considerablement developpee. Ces experiences sont interpretees dans le cadre de la theorie generale exposee precedemment. On prouve ainsi l'identite des parametres mesures

  6. Randomized control trial for the assessment of the anti-albuminuric effects of topiroxostat in hyperuricemic patients with diabetic nephropathy (the ETUDE study). (United States)

    Kato, Sawako; Ando, Masahiko; Mizukoshi, Toshihiro; Nagata, Takanobu; Katsuno, Takayuki; Kosugi, Tomoki; Tsuboi, Naotake; Maruyama, Shoichi


    Proteinuria is an established risk factor for diabetic nephropathy. Recent studies indicate that some xanthine oxidase inhibitors have a renoprotective effect. The aim of this study was to assess whether topiroxostat reduces albuminuria in hyperuricemic patients with diabetic nephropathy and overt proteinuria. The ETUDE study is an ongoing 24-week, multicenter, open-label, randomized (1:1), parallel group study involving hyperuricemic patients with diabetic nephropathy (estimated glomerular filtration rate [eGFR] ≥ 20 mL/min/1.73 m(2)) and overt proteinuria (0.3 ≤ urine protein to creatinine ratio (UPCR) ETUDE trial is the first randomized controlled study of topiroxostat in hyperuricemic patients with diabetic nephropathy and overt proteinuria. We will clarify the pleiotropic function of topiroxostat including an anti-albumiuric effect as well as its effects on safely decreasing serum uric acid levels.

  7. Adjunctive Therapies for Bacterial Keratitis. (United States)

    Dakhil, Turki Abdulaziz Bin; Stone, Donald U; Gritz, David C


    Bacterial keratitis is the most common type among all types of infectious keratitis. Currently, antibiotics are the main-stay of treatment. The objective of this systematic review is to review published clinical studies which discuss the adjunctive treatment of bacterial keratitis to guide clinical decision-making. We reviewed the role of a variety of medications and surgeries which can help in managing bacterial keratitis complications, which include as thinning, perforation, and impaired wound healing. We have included appropriate animal and laboratory studies, case reports and case series, and randomized clinical trials regarding each therapy.

  8. Molecular detection of human bacterial pathogens

    National Research Council Canada - National Science Library

    Liu, Dongyou


    .... Molecular Detection of Human Bacterial Pathogens addresses this issue, with international scientists in respective bacterial pathogen research and diagnosis providing expert summaries on current...

  9. Point-of-care ultrasonography adoption in Canada: using diffusion theory and the Evaluation Tool for Ultrasound skills Development and Education (ETUDE). (United States)

    Woo, Michael Y; Frank, Jason R; Lee, A Curtis


    Point-of-care ultrasonography (PoCUS) first appeared in the 1980s in North America, but the extent of the diffusion of its adoption is unknown. We characterized early PoCUS adoption by emergency physicians in Canada and its barriers to use using Rogers' diffusion of innovations theory. We developed a questionnaire based on a pilot study and literature review to assess past, current, and potential use of PoCUS and potential barriers to adoption. A Dillman technique for electronic surveys was used for dissemination. Using Rogers' diffusion of innovations theory, we developed and validated the Evaluation Tool for Ultrasound skills Development and Education (ETUDE). ETUDE scores allowed categorization of respondents into innovators, early adopters, majority, and nonadopters. Descriptive statistics, correlations, and χ² statistics were used to analyze the data. The 296 respondents (36.4% of 814 surveyed) had a median age of 40 and were 72.5% male. Adoption scores using ETUDE revealed nonadopters (18.8%), majority (28.7%), early adopters (34.5%), and innovators (18.0%). Respondents endorsed "always" using PoCUS currently and in the future for focused assessment with sonography in trauma (FAST) (current 41.8%/future 88.4%), first trimester pregnancy (current 23.3%/future 73.7%), suspected abdominal aortic aneurysm (current 32.7%/future 92.6%), basic cardiac indications (current 30.7%/future 87.5%), and central venous catheterization (current 17.0%/future 80.3%). Several barriers to PoCUS were identified for part-time emergency physicians and those working in inner-city/urban/suburban settings. This is the first study to determine the state of adoption and barriers to the introduction of PoCUS in Canadian emergency medicine practice. The novel validated ETUDE instrument should be used to evaluate the uptake of PoCUS over time.

  10. Etude de la transition ferroelectrique-ferroelastique du KD2PO4 forme du front de phase en fonction du gradient thermique


    Kvítek, Zdeněk


    Etude de la transition ferroelectrique-ferroelastique du KD2PO4 forme du front de phase en fonction du gradient thermique The thesis explores complex process of first order transition of KD2PO4 crystal from tetragonal phase to ferroelectric - ferroelastic orthorhombic phase and back at temperature 209 K. The experimental set up of nitrogeneous cryostat allowes temperature and temperature gradient variations during simultaneous three axes optical sample observations, dielectric measurements. T...

  11. Bacterial sepsis and chemokines. (United States)

    Kobayashi, Makiko; Tsuda, Yasuhiro; Yoshida, Tsuyoshi; Takeuchi, Dan; Utsunomiya, Tokuichiro; Takahashi, Hitoshi; Suzuki, Fujio


    Bacterial sepsis causes a high mortality rate when it occurs in patients with compromised host defenses. Severely burned patients, typical immunocompromised hosts, are extremely susceptible to infections from various pathogens, and a local wound infection frequently escalates into sepsis. In these patients, Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa are familiar pathogens that cause opportunistic infections. Also, polymicrobial sepsis frequently occurs in these patients. In this review, therefore, the roles of chemokines in thermally injured patients infected with these 3 pathogens and polymicrobial sepsis will be discussed. These infections in thermally injured patients may be controlled immunologically, because immunocompetent hosts are resistant to infections with these pathogens. Classically activated macrophages (M1Mphi) are major effector cells for host innate immune responses against these infections. However, M1Mphi are not generated in thermally injured patients whose alternatively activated macrophages (M2Mphi) predominate. M2Mphi appear in patients early after severe burn injuries. M2Mphi inhibit M1Mphi generation through the secretion of CCL17 and IL-10. As a modulator of Mphi, two different subsets of neutrophils (PMN-I, PMN-II) are described. PMN-I direct the polarization of resident Mphi into M1Mphi through the production of CCL3. M2Mphi are induced from resident Mphi by CCL2 released from PMN-II. Therefore, as an inhibitor of CCL2, glycyrrhizin protects individuals infected with S. aureus. Sepsis stemming from P. aeruginosa wound infection is also influenced by CCL2 released from immature myeloid cells. A large number of immature myeloid cells appear in association with burn injuries. Host resistance to S. aureus, E. faecalis, P. aeruginosa or polymicrobial infections may be improved in thermally injured patients through the induction of M1Mphi, elimination of CCL2 and/or depletion of M2Mphi induced by CCL2.

  12. Experimental contamination of margaritana margaritifera (L) (a Fresh water bivalve) by caesium 137; Contamination experimentale de margaritana margaritifera (L) (bivalve d'eau douce) par le cesium 137

    Energy Technology Data Exchange (ETDEWEB)

    Foulquier, L.; Bovard, P.; Grauby, A. [Commissariat a l' Energie Atomique, Cadarache (France). Centre d' Etudes Nucleaires


    The hydro biological research carried out in the Radio-Ecology Section has led the authors to study some Margaritana sampling stations situated down-stream from the Monts d'Arree nuclear power station. They describe the preservation and contamination methods used for fixing the {sup 137}Cs concentration factors in the case of Margaritana Margaritifera (L). The results of experiments carried out over a period of one hundred days show that the specific activity of the various organs is stabilized after thirty to thirty-five days. The authors have noticed a relatively low adsorption on the shell through the intermediary of micro-organisms, and a strong and rapid absorption in the soft parts. The concentration factors have values, at equilibrium, of around: 9 for the shell, 300 for all the organs, and 38 for the whole animal. A comparison of these results with work published by other authors makes it possible to draw general conclusions concerning the mechanism of {sup 137}Cs fixation by lamellibranch, as well as their capacity of fixation. (author) [French] Les etudes hydrobiologiques effectuees au sein de la Section de Radio-Ecologie ont amene les auteurs a etudier des stations de prelevement de Margaritana en aval de la Centrale Nucleaire des Monts d'Arree. Ils decrivent les methodes de conservation et de contamination utilisees pour l'etablissement des facteurs de concentration du {sup 137}Cs par Margaritana margaritifera (L). Les resultats des experimentations menees pendant cent jours montrent que les activites specifiques de la coquille et des differents organes se stabilisent au bout de trente a trente-cinq jours. Les auteurs constatent une adsorption relativement faible sur la coquille par l'intermediaire des micro-organismes et une absorption forte et rapide dans les parties molles. Les facteurs de concentration se situent, a l'equilibre, autour de: 9 pour la coquille, 300 pour l'ensemble des organes et 38 si l'on considere

  13. A study of the aptitude of soils under natural conditions to retain radiostrontium; Etude de la vocation des sols en place a la retention du radiostrontium

    Energy Technology Data Exchange (ETDEWEB)

    Bovard, P.; Grauby, A. [Commissariat a l' Energie Atomique, Saclay (France).Centre d' Etudes Nucleaires


    Independently of the theoretical study of the propagation of radioactivity in the soil as a result of submersions or of radioactive rain, the authors have studied directly and practically how this radioactivity can vary in the actual soil. To this end a simple, rapid method has been perfected; it makes it possible to maintain for each soil sample the natural parameters (structure, humidity, etc.) without introducing boundary effects. In the laboratory, after charging the soil samples, part of the study of the propagation of radioactivity is done by autoradiography; finally, as a practical application, the study of an atomic site illustrates the methods described. (author) [French] Independamment de l'etude theorique de la propagation de la radioactivite dans le sol a la suite de submersions ou de pluies radioactives, les auteurs ont etudie directement et pratiquement comment pourrait evoluer cette radioactivite dans les sols en place. Pour cela, une methode simple et rapide a ete mise au point; elle permet de conserver pour chaque echantillon de sol, les parametres naturels (structure, humidite, etc...), sans introduire d'effets de paroi. En laboratoire, apres mise en charge des massifs preleves, une partie de l'etude de la propagation des radioelements est realisee par autoradiographie; enfin, une application pratique, l'etude d'un site atomique, illustre l'expose. (auteur)

  14. The development of a very high stability electrostatic generator (1962); Etude et realisation d'un generateur electrostatique a tres haute stabilite (1962)

    Energy Technology Data Exchange (ETDEWEB)

    Jonckheere, R.E.L. [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires


    This thesis deals with the study of an electrostatic high voltage generator having a voltage stability of the order of 10{sup -6} per minute. This equipment should be very useful in electron microscopy. The electrostatic generator is studied as a control system element: transfer function, parasitic signals and noise are determined and a mathematical model is proposed. A theoretical study of the open loop transfer function, stability, transient response, voltage stabilization of five different control systems shows which one should be able to fulfill the requirements There follows a detailed study of drift, a description of the actual system and performance data. (author) [French] Cette etude concerne un generateur electrostatique capable de fournir une tres haute tension continue dont la stabilite relative est de l'ordre de 10{sup -6} pendant une minute. Une telle performance rend cet appareillage tres utile en microscopie electronique. La generatrice electrostatique est etudiee en tant qu'element d'un systeme asservi: on determine successivement la fonction de transfert, les perturbations, le bruit de fond et le modele mathematique. L'etude de cinq differents circuits de regulation en ce qui concerne leur fonction de transfert, stabilite, reponse en regime transitoire, attenuation des perturbations, permettra de choisir le systeme qui semble le mieux repondre aux exigences. Viennent ensuite une etude detaillee de la derive, la description de la realisation pratique et les resultats de mesure. (auteur)

  15. Bacterial Communities: Interactions to Scale

    Directory of Open Access Journals (Sweden)

    Reed M. Stubbendieck


    Full Text Available In the environment, bacteria live in complex multispecies communities. These communities span in scale from small, multicellular aggregates to billions or trillions of cells within the gastrointestinal tract of animals. The dynamics of bacterial communities are determined by pairwise interactions that occur between different species in the community. Though interactions occur between a few cells at a time, the outcomes of these interchanges have ramifications that ripple through many orders of magnitude, and ultimately affect the macroscopic world including the health of host organisms. In this review we cover how bacterial competition influences the structures of bacterial communities. We also emphasize methods and insights garnered from culture-dependent pairwise interaction studies, metagenomic analyses, and modeling experiments. Finally, we argue that the integration of multiple approaches will be instrumental to future understanding of the underlying dynamics of bacterial communities.

  16. Bacterial flora of sturgeon fingerling

    International Nuclear Information System (INIS)

    Arani, A.S.; Mosahab, R.


    The study on microbial populations is a suitable tool to understand and apply control methods to improve the sanitary level of production in fish breeding and rearing centers, ensure health of sturgeon fingerlings at the time of their release into the rivers and also in the conversation and restoration of these valuable stocks in the Caspian Sea, Iran. A laboratory research based on Austin methods (Austin, B., Austin, D.A. 1993) was conducted for bacterial study on 3 sturgeon species naming A. persicus, A. stellatus and A. nudiventris during different growth stages. Bacterial flora of Acinetobacter, Moraxella, Aeromonas, Vibrio, Edwardsiella, Staphylococcus, Proteus, Yersinia, Pseudomonas and Plesiomonas were determined. The factors which may induce changes in bacterial populations during different stages of fife are the followings: quality of water in rearing ponds, different conditions for growth stages, suitable time for colonization of bacterial flora in rearing pond, water temperature increase in fingerlings size and feeding condition. (author)

  17. Subdural Empyema in Bacterial Meningitis

    Directory of Open Access Journals (Sweden)

    J Gordon Millichap


    Full Text Available Researchers at the University of Amsterdam, the Netherlands, evaluated the occurrence, treatment, and outcome of subdural empyema as a complication of community-acquired bacterial meningitis in 28 (2.7% adults.

  18. Arsenic uptake in bacterial calcite

    Energy Technology Data Exchange (ETDEWEB)

    Catelani, Tiziano; Perito, Brunella; Bellucci, Francesco; Lee, Sang Soo; Fenter, Paul; Newville, Matthew G.; Rimondi, Valentina; Pratesi, Giovanni; Costagliola, Pilario


    Bio-mediated processes for arsenic (As) uptake in calcite were investigated by means of X-ray Diffraction (XRD) and Xray Absorption Spectroscopy (XAS) coupled with X-ray Fluorescence measurements. The environmental bacterial strain Bacillus licheniformis BD5, sampled at the Bullicame Hot Springs (Viterbo, Central Italy), was used to synthesize calcite from As-enriched growth media. Both liquid and solid cultures were applied to simulate planktonic and biofilm community environments, respectively. Bacterial calcite samples cultured in liquid media had an As enrichment factor (Kd) 50 times higher than that from solid media. The XRD analysis revealed an elongation of the crystal lattice along the c axis (by 0.03Å) for biogenic calcite, which likely resulted from the substitution of larger arsenate for carbonate in the crystal. The XAS data also showed a clear difference in the oxidation state of sorbed As between bacterial and abiotic calcite. Abiotic chemical processes yielded predominantly As(V) uptake whereas bacterial precipitation processes led to the uptake of both As(III) and As(V). The presence of As(III) in bacterial calcite is proposed to result from subsequent reduction of arsenate to arsenite by bacterial activities. To the best of our knowledge, this is the first experimental observation of the incorporation of As(III) in the calcite crystal lattice, revealing a critical role of biochemical processes for the As cycling in nature.

  19. Arsenic uptake in bacterial calcite (United States)

    Catelani, Tiziano; Perito, Brunella; Bellucci, Francesco; Lee, Sang Soo; Fenter, Paul; Newville, Matthew; Rimondi, Valentina; Pratesi, Giovanni; Costagliola, Pilario


    Bio-mediated processes for arsenic (As) uptake in calcite were investigated by means of X-ray Diffraction (XRD) and X-ray Absorption Spectroscopy (XAS) coupled with X-ray Fluorescence measurements. The environmental bacterial strain Bacillus licheniformis BD5, sampled at the Bullicame Hot Springs (Viterbo, Central Italy), was used to synthesize calcite from As-enriched growth media. Both liquid and solid cultures were applied to simulate planktonic and biofilm community environments, respectively. Bacterial calcite samples cultured in liquid media had an As enrichment factor (Kd) 50 times higher than that from solid media. The XRD analysis revealed an elongation of the crystal lattice along the c axis (by 0.03 Å) for biogenic calcite, which likely resulted from the substitution of larger arsenate for carbonate in the crystal. The XAS data also showed a clear difference in the oxidation state of sorbed As between bacterial and abiotic calcite. Abiotic chemical processes yielded predominantly As(V) uptake whereas bacterial precipitation processes led to the uptake of both As(III) and As(V). The presence of As(III) in bacterial calcite is proposed to result from subsequent reduction of arsenate to arsenite by bacterial activities. To the best of our knowledge, this is the first experimental observation of the incorporation of As(III) in the calcite crystal lattice, revealing a critical role of biochemical processes for the As cycling in nature.

  20. Current knowledge of bacterial vaginosis

    Directory of Open Access Journals (Sweden)

    Đukić Slobodanka


    Full Text Available Bacterial vaginosis, earlier termed nonspecific vaginitis (anaerobic vaginosis because of the absence of recognized pathogens, is most common vaginal syndrome of women of childbearing age affecting 15-30%. This syndrome, whose aetiology and pathogenesis remains unknown, is characterized by significant changes in the vaginal ecosystem. These changes consist of a decrease in the number of lactobacilli and a large increase in the number of anaerobic organisms. The bacteria adhere to desquamated epithelial cells with a distinctive appearance of clue cells The main complaints of women with symptomatic bacterial vaginosis include vaginal discharge and odour. However, a significant number of all women who have bacterial vaginosis deny symptoms. Bacterial vaginosis is associated with a number of gynaecologic and obstetric complications including cervicitis, cervical neoplasia, pelvic inflammatory disease, postoperative infections, and preterm labour. The diagnosis is most frequently made based on vaginal smear stained according to Gram (Nugent scoring method. Metronidazole and clindamycin are the drugs of choice for treatment of women with bacterial vaginosis. Which women should undergo treatment? According to the prevailing attitude, it should include women with symptoms. Symptomatic women with frequent relapses of bacterial vaginosisas, as a rule, have poor response to the applied therapy. To achieve better efficiency in the treatment of such women, it is necessary to have more extensive understanding of all factors in the pathogenesis of the syndrome.

  1. Study of radio-active ions in the atmosphere; Etude des ions radioactifs de l'atmosphere

    Energy Technology Data Exchange (ETDEWEB)

    Renoux, A. [Commissariat a l' Energie Atomique, Fontenay-aux-Roses (France). Centre d' Etudes Nucleaires


    A comparative study is made of active, deposits of radon and thoron in suspension in the atmosphere by means of {alpha} radiation counting, using ZELENY tubes, scattering equipment, filter papers or membranes. It has been possible to show the existence of small and large ions which are negative and positive, as well as of neutral radio-active nuclei; their properties are studied. A theoretical interpretation of the results is presented. The average content of radon (using the Ra A concentration) and of Th B in the air has been determined. The radioactive equilibrium between radon and its daughter products in atmospheric air are examined. The techniques developed for active radon and thoron deposits are applied to the study of artificial radio-activity, the analyses being carried out by means of {gamma} spectrometry. (author) [French] On effectue une etude comparative entre les depots actifs du radon et du thoron en suspension dont l'atmosphere a l'aide de comptages de rayonnement {alpha}, en utilisant des tubes de ZELENY, une batterie de diffusion, des papiers filtres ou des membranes. On met ainsi en evidence la presence de petits et gros ions negatifs et positifs, ainsi que celle de noyaux neutres radioactifs, et on etudie leurs proprietes. Une Interpretation theorique des resultats obtenus est developpee. On determine la teneur moyenne de l'air en radon (a partir de la concentration en Ra A) et en Th B. L'equilibre radioactif entre le radon et ses descendants, dans l'air atmospherique, est examine. Les Techniques mises au point pour les depots actifs du radon et du thoron sont appliquees a l'etude de la radioactivite artificielle, les depouillements s'effectuant par spectrometrie {gamma}. (auteur)

  2. Study of thermocouples for control of high temperatures; Etude de thermocouples pour le reperage des hautes temperatures

    Energy Technology Data Exchange (ETDEWEB)

    Villamayor, M. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires; Faculte des Sciences de l' Universite de Lyon - 69 (France)


    Previous works have shown that the tungsten-rhenium alloys thermocouples were a good instrument for control of high temperatures. From its, the author has studied the W/W 26 per cent and W 5 per cent Re/W 26 per cent Re french manufactured thermocouples and intended for control of temperatures in nuclear reactors until 2300 deg. C. In 'out-pile' study he determines the general characteristics of these thermocouples: average calibration curves, thermal shocks influence, response times, and alloys allowing the cold source compensation. The evolution of these thermocouples under thermal neutron flux has been determined by 'in-pile' study. The observations have led the author to propose a new type of thermocouples settled of molybdenum-columbium alloys. (author) [French] Des travaux anterieurs ont montre que les thermocouples des alliages tungstene-rhenium etaient susceptibles de reperer avec precision des hautes temperatures. A partir de la, l'auteur a etudie las thermocouples W/W 26 pour cent Re et W 5 pour cent Re/W 26 pour cent Re de fabrication francaise et destines au controle des temperatures dans les reacteurs nucleaires, jusqu'a 2300 deg. C Dans l'etude 'hors-pile' il a determine les caracteristiques generales de ces thermocouples: courbes d'etalonnage moyen, influence des chocs thermiques, temps de reponse, et alliages assurant la compensation de soudure froide. L'etude 'en-pile' a permis de rendre compte de l'evolution de ces thermocouples sous flux neutroniques. Les phenomenes observes ont conduit l'auteur a proposer un nouveau type de thermocouples constitues d'alliages molybdene-niobium. (auteur)

  3. Bacterial Prostatitis: Bacterial Virulence, Clinical Outcomes, and New Directions. (United States)

    Krieger, John N; Thumbikat, Praveen


    Four prostatitis syndromes are recognized clinically: acute bacterial prostatitis, chronic bacterial prostatitis, chronic prostatitis/chronic pelvic pain syndrome, and asymptomatic prostatitis. Because Escherichia coli represents the most common cause of bacterial prostatitis, we investigated the importance of bacterial virulence factors and antimicrobial resistance in E. coli strains causing prostatitis and the potential association of these characteristics with clinical outcomes. A structured literature review revealed that we have limited understanding of the virulence-associated characteristics of E. coli causing acute prostatitis. Therefore, we completed a comprehensive microbiological and molecular investigation of a unique strain collection isolated from healthy young men. We also considered new data from an animal model system suggesting certain E. coli might prove important in the etiology of chronic prostatitis/chronic pelvic pain syndrome. Our human data suggest that E. coli needs multiple pathogenicity-associated traits to overcome anatomic and immune responses in healthy young men without urological risk factors. The phylogenetic background and accumulation of an exceptional repertoire of extraintestinal pathogenic virulence-associated genes indicate that these E. coli strains belong to a highly virulent subset of uropathogenic variants. In contrast, antibiotic resistance confers little added advantage to E. coli strains in these healthy outpatients. Our animal model data also suggest that certain pathogenic E. coli may be important in the etiology of chronic prostatitis/chronic pelvic pain syndrome through mechanisms that are dependent on the host genetic background and the virulence of the bacterial strain.


    Directory of Open Access Journals (Sweden)

    Sheila Cristina Monteiro Matos


    Full Text Available Este estudio tuvo como objetivo analizar cómo se está desarrollando el conocimiento experimental en las prácticas educativas de la Escuela Municipal Visconde de Itaboraí, en el municipio de Duque de Caxias, desde la aplicación del Programa “Más Educación/Más Escuela”. Para ello, se utilizó el método dialéctico impartido por Frigotto, teniendo como método de análisis el estudio de caso. El trabajo de campo se desarrolló a través de entrevistas, observaciones y la investigación de registros. El análisis de datos se realizó mediante análisis de contenido según Bardin. El estudio de la práctica educativa y del conocimiento experimental tuve como referencia las enseñanzas de Tardif sobre el conocimiento docente. En síntesis, podemos decir que los conocimientos experimentales en las prácticas educativas promueven la formación integral, proporcionando un mejor rendimiento académico de los estudiantes, aunque tímidamente, pues la articulación de las materias entre los turnos es un obstáculo importante para la efectividad de las prácticas educativas.

  5. Aproximación al estudio de las estrategias didácticas en ciencias experimentales en formación inicial del profesorado de Educación Secundaria: descripción de una experiencia

    Directory of Open Access Journals (Sweden)

    Natalia Jiménez-Tenorio


    Full Text Available Se fundamenta y describe una actividad orientada a la formación inicial de profesores de secundaria de especialidades de ciencias experimentales. La actividad está planteada como intento de aproximación al estudio de distintas estrategias didácticas, más o menos insertas dentro del marco socio-constructivista: “aprendizaje por descubrimiento”, “cambio conceptual”, “enseñanza por investigación entorno a problemas” y “enfoques CTS”. La actividad incluye una serie de estudios de caso (cuatro en total, uno para cada estrategia analizada consistente en supuestos de secuencias de actividades dirigidas a alumnos de educación secundaria obligatoria. Acompañando a los cuatro casos se proporciona un guión de análisis consistente en una serie de preguntas que los alumnos tienen que responder, como forma de orientar el trabajo y de articular el desarrollo de la actividad en su conjunto. La actividad fue ensayada con grupos de alumnos del MAES (Máster de Educación Secundaria de especialidades de “Física y Química” y de “Biología y geología”, y sus resultados expuestos en otro estudio publicado en paralelo a éste.

  6. The Human Vaginal Bacterial Biota and Bacterial Vaginosis

    Directory of Open Access Journals (Sweden)

    Sujatha Srinivasan


    Full Text Available The bacterial biota of the human vagina can have a profound impact on the health of women and their neonates. Changes in the vaginal microbiota have been associated with several adverse health outcomes including premature birth, pelvic inflammatory disease, and acquisition of HIV infection. Cultivation-independent molecular methods have provided new insights regarding bacterial diversity in this important niche, particularly in women with the common condition bacterial vaginosis (BV. PCR methods have shown that women with BV have complex communities of vaginal bacteria that include many fastidious species, particularly from the phyla Bacteroidetes and Actinobacteria. Healthy women are mostly colonized with lactobacilli such as Lactobacillus crispatus, Lactobacillus jensenii, and Lactobacillus iners, though a variety of other bacteria may be present. The microbiology of BV is heterogeneous. The presence of Gardnerella vaginalis and Atopobium vaginae coating the vaginal epithelium in some subjects with BV suggests that biofilms may contribute to this condition.

  7. Bacterial contamination of blood products. (United States)

    Palavecino, Elizabeth; Jacobs, Michael; Yomtovian, Roslyn


    The occurrence of a septic reaction resulting from bacterial contamination of blood products, particularly with room-temperature stored platelets, is the most common transfusion-associated infectious risk in the United States. Bacterial contamination of blood products was first identified more than 60 years ago; yet, strategies to resolve this problem have proved daunting despite ongoing awareness and increasing concern especially in the last few years. With the recent US Food and Drug Administration (FDA) approval of culture methods for quality control testing of platelet units and the promulgation of accreditation standards by the College of American Pathologists and American Association of Blood Banks to detect bacterially contaminated platelet units and to prevent transfusion of these units, blood banks and transfusion services have finally started to address this problem, in a more standardized manner. Furthermore, as new methods of interdicting, inactivating and detecting bacterially contaminated blood products emerge, it is hoped that the problem of bacterial contamination of blood products will be overcome.

  8. Community-acquired bacterial meningitis. (United States)

    Costerus, Joost M; Brouwer, Matthijs C; Bijlsma, Merijn W; van de Beek, Diederik


    Bacterial meningitis is a medical emergency and is associated with a high disease burden. We reviewed recent progress in the management of patients with community-acquired bacterial meningitis. The worldwide burden of disease of bacterial meningitis remains high, despite the decreasing incidence following introduction of routine vaccination campaigns. Delay in diagnosis and treatment remain major concerns in the management of acute bacterial meningitis. European Society of Clinical Microbiology and Infectious Diseases guidelines strive for a door-to-antibiotic-time less than 1 h. Polymerase chain reaction (PCR) has emerged as an important diagnostic tool to identify the causative organism. Point-of-care tests using fast multiplex PCR have been developed, but additional value has not been proven. Although anecdotal observations advocate pressure-based management, a randomized controlled trial will need to be performed first to determine efficacy and safety of such an aggressive treatment approach. Adjunctive dexamethasone remains the only adjunctive therapy with proven efficacy. The incidence of bacterial meningitis has been decreasing after the implementation of effective vaccines. Treatment should be administered as soon as possible and time to treatment should not exceed 1 h.

  9. Abdominal radiation causes bacterial translocation

    International Nuclear Information System (INIS)

    Guzman-Stein, G.; Bonsack, M.; Liberty, J.; Delaney, J.P.


    The purpose of this study was to determine if a single dose of radiation to the rat abdomen leads to bacterial translocation into the mesenteric lymph nodes (MLN). A second issue addressed was whether translocation correlates with anatomic damage to the mucosa. The radiated group (1100 cGy) which received anesthesia also was compared with a control group and a third group which received anesthesia alone but no abdominal radiation. Abdominal radiation lead to 100% positive cultures of MLN between 12 hr and 4 days postradiation. Bacterial translocation was almost nonexistent in the control and anesthesia group. Signs of inflammation and ulceration of the intestinal mucosa were not seen until Day 3 postradiation. Mucosal damage was maximal by Day 4. Bacterial translocation onto the MLN after a single dose of abdominal radiation was not apparently dependent on anatomical, histologic damage of the mucosa

  10. Bacterial Degradation of Aromatic Compounds

    Directory of Open Access Journals (Sweden)

    Qing X. Li


    Full Text Available Aromatic compounds are among the most prevalent and persistent pollutants in the environment. Petroleum-contaminated soil and sediment commonly contain a mixture of polycyclic aromatic hydrocarbons (PAHs and heterocyclic aromatics. Aromatics derived from industrial activities often have functional groups such as alkyls, halogens and nitro groups. Biodegradation is a major mechanism of removal of organic pollutants from a contaminated site. This review focuses on bacterial degradation pathways of selected aromatic compounds. Catabolic pathways of naphthalene, fluorene, phenanthrene, fluoranthene, pyrene, and benzo[a]pyrene are described in detail. Bacterial catabolism of the heterocycles dibenzofuran, carbazole, dibenzothiophene, and dibenzodioxin is discussed. Bacterial catabolism of alkylated PAHs is summarized, followed by a brief discussion of proteomics and metabolomics as powerful tools for elucidation of biodegradation mechanisms.

  11. Bacterial computing with engineered populations. (United States)

    Amos, Martyn; Axmann, Ilka Maria; Blüthgen, Nils; de la Cruz, Fernando; Jaramillo, Alfonso; Rodriguez-Paton, Alfonso; Simmel, Friedrich


    We describe strategies for the construction of bacterial computing platforms by describing a number of results from the recently completed bacterial computing with engineered populations project. In general, the implementation of such systems requires a framework containing various components such as intracellular circuits, single cell input/output and cell-cell interfacing, as well as extensive analysis. In this overview paper, we describe our approach to each of these, and suggest possible areas for future research. © 2015 The Author(s) Published by the Royal Society. All rights reserved.

  12. Les Ethmoidites Aigues Exteriorisees De L\\'enfant Etude De 11 ...

    African Journals Online (AJOL)

    They are the most frequent among bacterial sinusitis before 3 years. Population and methods:Our study is retrospective, it included all children hospitalized in our pediatric unit from 2000 to 2006 for an acute ethmoiditis. We analyzed clinical and radiological presentations, and discussed therapeutic modalities used to treat ...

  13. Biotechnological applications of bacterial cellulases

    Czech Academy of Sciences Publication Activity Database

    Menéndez, E.; García-Fraile, Paula; Rivas, R.


    Roč. 2, č. 3 (2015), s. 163-182 ISSN 2306-5354 R&D Projects: GA MŠk(CZ) EE2.3.30.0003 Institutional support: RVO:61388971 Keywords : Biotechnological applications * Bacterial cellulases * Cellulose degradation Subject RIV: EE - Microbiology, Virology

  14. Disease notes - Bacterial root rot (United States)

    Bacterial root rot initiated by lactic acid bacteria, particularly Leuconostoc, occurs every year in Idaho sugarbeet fields. Hot fall weather seems to make the problem worse. Although Leuconostoc initiates the rot, other bacteria and yeast frequently invade the tissue as well. The acetic acid bac...

  15. Metagenomic Diagnosis of Bacterial Infections (United States)

    Nakamura, Shota; Maeda, Norihiro; Miron, Ionut Mihai; Yoh, Myonsun; Izutsu, Kaori; Kataoka, Chidoh; Honda, Takeshi; Yasunaga, Teruo; Nakaya, Takaaki; Kawai, Jun; Hayashizaki, Yoshihide; Horii, Toshihiro


    To test the ability of high-throughput DNA sequencing to detect bacterial pathogens, we used it on DNA from a patient’s feces during and after diarrheal illness. Sequences showing best matches for Campylobacter jejuni were detected only in the illness sample. Various bacteria may be detectable with this metagenomic approach. PMID:18976571

  16. bacterial flora and antibiotic sensitivity

    African Journals Online (AJOL)

    Purulent pelvic collections are common pathologies observed in contemporary gynaecological practice. They may originate from chronic pelvic inflammatory disease, from abortions or following normal deliveries. This study was designed to compare the bacterial flora in purulent pelvic collections obtained from HIV infected ...

  17. Community-acquired bacterial meningitis

    NARCIS (Netherlands)

    van de Beek, Diederik; Brouwer, Matthijs; Hasbun, Rodrigo; Koedel, Uwe; Whitney, Cynthia G.; Wijdicks, Eelco


    Meningitis is an inflammation of the meninges and subarachnoid space that can also involve the brain cortex and parenchyma. It can be acquired spontaneously in the community - community-acquired bacterial meningitis - or in the hospital as a complication of invasive procedures or head trauma

  18. Molecular mechanisms underlying bacterial persisters

    DEFF Research Database (Denmark)

    Maisonneuve, Etienne; Gerdes, Kenn


    All bacteria form persisters, cells that are multidrug tolerant and therefore able to survive antibiotic treatment. Due to the low frequencies of persisters in growing bacterial cultures and the complex underlying molecular mechanisms, the phenomenon has been challenging to study. However, recent...

  19. Biotechnological applications of bacterial cellulases

    Directory of Open Access Journals (Sweden)

    Esther Menendez


    Full Text Available Cellulases have numerous applications in several industries, including biofuel production, food and feed industry, brewing, pulp and paper, textile, laundry, and agriculture.Cellulose-degrading bacteria are widely spread in nature, being isolated from quite different environments. Cellulose degradation is the result of a synergic process between an endoglucanase, an exoglucanase and a,β-glucosidase. Bacterial endoglucanases degrade ß-1,4-glucan linkages of cellulose amorphous zones, meanwhile exoglucanases cleave the remaining oligosaccharide chains, originating cellobiose, which is hydrolyzed by ß-glucanases. Bacterial cellulases (EC are comprised in fourteen Glycosil Hydrolase families. Several advantages, such as higher growth rates and genetic versatility, emphasize the suitability and advantages of bacterial cellulases over other sources for this group of enzymes. This review summarizes the main known cellulolytic bacteria and the best strategies to optimize their cellulase production, focusing on endoglucanases, as well as it reviews the main biotechnological applications of bacterial cellulases in several industries, medicine and agriculture.

  20. Etude thermo-hydraulique de l'ecoulement du moderateur dans le reacteur CANDU-6 (United States)

    Mehdi Zadeh, Foad

    importante, se traduisant par l'accroissement de la puissance du reacteur. Ceci peut avoir des consequences majeures pour la surete nucleaire. Une modelisation CFD (Computational Fluid Dynamics) detaillee tenant compte des effets locaux s'avere donc necessaire. Le but de ce travail de recherche est de modeliser le comportement complexe de l'ecoulement du moderateur au sein de la cuve d'un reacteur nucleaire CANDU-6, notamment a proximite des tubes de calandre. Ces simulations servent a identifier les configurations possibles de l'ecoulement dans la calandre. Cette etude consiste ainsi a formuler des bases theoriques a l'origine des instabilites macroscopiques du moderateur, c.-a-d. des mouvements asymetriques qui peuvent provoquer l'ebullition du moderateur. Le defi du projet est de determiner l'impact de ces configurations de l'ecoulement sur la reactivite du reacteur CANDU-6.

  1. La structure de l'eau liquide: Une etude thermique par spectroscopie infrarouge (United States)

    Larouche, Pascal

    Le probleme de la structure de l'eau liquide est important car l'eau est le liquide le plus present sur Terre, et complexe, la quete d'un modele precis pour decrire comment fonctionne ce liquide ayant debute des la fin du dix-neuvieme siecle. Cette etude aborde ce probleme en etudiant l'effet de l'augmentation de la temperature sur H2O et D 2O purs a l'aide de la spectroscopie infrarouge. L'intervalle de temperatures scrute est 29--93.1°C. Les spectres enregistres sont des spectres MIR-ATR entre 650 et 6000 cm-1 . L'analyse par facteurs de ces donnees permet de montrer que deux et seulement deux facteurs principaux sont necessaires pour decomposer tous les spectres experimentaux. Ces resultats sont confirmes grace a l'analyse par facteurs de spectres de la region FIR. Par la suite, la transformation en spectres de la partie reelle n et imaginaire k de l'indice de refraction permet de combiner les donnees des regions MIR et FIR. Une fois ce calcul termine, les spectres de transmission complets de H 2O et D2O entre 25 et 90°C sont connus. Ils sont ensuite utilises pour calculer par extrapolation le spectre des especes constituant l'eau liquide, puis leur abondance en fonction de la temperature. L'extrapolation de ces abondances montre que les especes correspondent a des temperatures limites de --18 et 122°C. Par la suite, la decomposition gaussienne des spectres d'especes met en evidence la riche structure de ces objets et permet de demontrer que l'apparent deplacement du massif d'absorption OH (OD) est produit par une variation de l'intensite des bandes et non pas de leur deplacement. L'examen attentif des spectres des especes prouve qu'il n'y a pas de OH libres crees par l'augmentation de la temperature: meme a 93.1°C, chaque molecule possede quatre liens-H. Ces conclusions sont de plus confirmees par une analyse thermodynamique du passage des molecules de la phase solide a la phase gazeuse. Pour diversifier la nature des resultats experimentaux utilises, des

  2. The oscillatory behavior of heated channels: an analysis of the density effect. Part I. The mechanism (non linear analysis). Part II. The oscillations thresholds (linearized analysis); Sur le comportement oscillatoire de canaux chauffes. - Etude theorique de l'effet de densite. 1ere partie: le mecanisme (analyse non lineaire), 2eme partie: seuils d'oscillation (analyse lineaire)

    Energy Technology Data Exchange (ETDEWEB)

    Boure, J. [Commissariat a l' Energie Atomique, Centre d' Etudes Nucleaires de Grenoble, 38 (France)


    The problem of the oscillatory behavior of heated channels is presented in terms of delay-times and a density effect model is proposed to explain the behavior. The density effect is the consequence of the physical relationship between enthalpy and density of the fluid. In the first part non-linear equations are derived from the model in a dimensionless form. A description of the mechanism of oscillations is given, based on the analysis of the equations. An inventory of the governing parameters is established. At this point of the study, some facts in agreement with the experiments can be pointed out. In the second part the start of the oscillatory behavior of heated channels is studied in terms of the density effect. The threshold equations are derived, after linearization of the equations obtained in Part I. They can be solved rigorously by numerical methods to yield: -1) a relation between the describing parameters at the onset of oscillations, and -2) the frequency of the oscillations. By comparing the results predicted by the model to the experimental behavior of actual systems, the density effect is very often shown to be the actual cause of oscillatory behaviors. (author) [French] Premiere partie: mecanisme (equations non linearisees). On expose le probleme du comportement oscillatoire des canaux chauffes en mettant l'accent sur la presence de retards dans le systeme et on propose un modele a 'effet de densite' pour expliquer ce comportement. L'effet de densite est la consequence de la relation physique entre l'enthalpie et la masse volumique du fluide. Les equations non lineaires du schema mathematique correspondant sont etablies et mises sous forme adimensionnelle. L'analyse de ces equations conduit a une description du mecanisme des oscillations. On donne la liste des parametres dont depend le comportement du modele. A ce stade de l'etude, on peut deja relever dans ce comportement plusieurs faits conformes a l

  3. Variabilidad de las proporciones molares en poblaciones humanas: un abordaje empleando modelos del desarrollo y experimentales / Variability of molar proportions in human populations: insights from developmental models and experiments in mice

    Directory of Open Access Journals (Sweden)

    Lucas A. D´Addona


    Full Text Available Los datos sobre la variación dental interpoblacional y sobre los mecanismos que regulan el desarrollo dental aportados por estudios experimentales aún no han sido integrados en el contexto de investigaciones antropobiológicas. En este sentido, el presente trabajo se propone: a evaluar la consistencia entre la variación de las proporciones molares en poblaciones humanas que exhiben gran variación en el tamaño dental con las predicciones derivadas de un modelo de desarrollo dental de cascada inhibitoria, y b analizar el efecto de los factores sistémicos que controlan el crecimiento del organismo sobre las proporciones de los molares inferiores empleando cepas de Mus musculus. Se calcularon las áreas de los molares inferiores mediante los diámetros bucolingual y mesiodistal. La variación interpoblacional en las proporciones M2/M1 y M3/M1 fue concordante con las expectativas derivadas del modelo y la mayoría de los grupos exhibieron una tendencia a la reducción del tamaño en sentido antero-posterior. Asimismo, se observó una asociación significativa y positiva entre el área molar total y las proporciones molares. En los modelos experimentales la alteración del crecimiento por factores sistémicos (subnutrición proteica y reducción de la hormona de crecimiento resultó en la disminución del área molar total y cambios en las proporciones molares. Estos últimos, consistentes con el incremento del efecto inhibitorio en sentido antero-posterior. En conjunto, los resultados sugieren que la modificación de los factores sistémicos que regulan el área molar total podría conducir a cambios en la proporción de activadores-inhibidores y contribuir a la diferenciación interpoblacional en las proporciones molares. Palabras clave: Modelo de cascada inhibitoria; tamaño dental; aproximación experimental y comparativa Data on dental variation among human populations and the mechanisms regulating tooth development elucidated by

  4. Evaluation of experimental vaccines for bovine viral diarrhea in bovines, ovines and guinea pigs Evaluación de vacunas experimentales para la diarrea viral bovina en bovinos, ovinos y cobayos

    Directory of Open Access Journals (Sweden)

    F. Fernández


    Full Text Available The bovine viral diarrhea virus (BVDV infection control should be based on elimination of persistently infected animals and on immunization through vaccination, to prevent fetal infection. However, the efficacy of inactivated BVDV vaccines is variable due to its low immunogenicity. This study evaluated the humoral immune response against homologous and heterologous strains of 7 inactivated BVDV vaccines, in bovines and two experimental models (ovine and guinea pig which might be used to test candidate vaccines. Vaccines formulated with BVDV Singer, Oregon, NADL, and multivalent, induced seroconversion in the three animal models studied, reaching antibody titres higher than 2. Vaccine containing 125C -genotype 2- only induced a low antibody response in ovine, while VS-115 NCP vaccine was not immunogenic. Furthermore, bovine sera at 60 dpv presented homologous as well as heterologous antibody response, indicating a high degree of cross-reactivity among the strains studied. However, when bovine sera were tested against the Argentine field strain 00-693, they showed the lowest levels of cross-reactivity, suggesting the need of continued surveillance to identify and characterize emerging field BVDV strains. Finally, optimal correlations between bovine-ovine and bovine-guinea pig models were observed, indicating that two alternative species could replace bovines when testing the immunogenicity of BVDV candidate vaccines.El control del virus de la diarrea viral bovina (VDVB se basa en la eliminación de animales persistentemente infectados, y la inmunización de hembras para prevenir infecciones fetales. La eficiencia de estas vacunas es variable por su baja inmunogenicidad. Se evaluó la respuesta inmune humoral contra virus homólogos y heterólogos de 7 vacunas experimentales inactivadas del VDVB en bovinos y en dos modelos experimentales (ovinos y cobayos. Las vacunas conteniendo VDVB Singer, Oregon, NADL y polivalentes indujeron seroconversión en

  5. Bacterial cells with improved tolerance to polyamines

    DEFF Research Database (Denmark)


    Provided are bacterial cells genetically modified to improve their tolerance to certain commodity chemicals, such as polyamines, and methods of preparing and using such bacterial cells for production of polyamines and other compounds....

  6. Bacterial cells with improved tolerance to polyols

    DEFF Research Database (Denmark)


    The present invention relates to bacterial cells genetically modified to improve their tolerance to certain commodity chemicals, such as diols and other polyols, and to methods of preparing and using such bacterial cells for production of polyols and other compounds....

  7. Adjunctive Corticosteroids in Adults with Bacterial Meningitis

    NARCIS (Netherlands)

    van de Beek, Diederik; de Gans, Jan


    Bacterial meningitis is a complex disorder in which neurologic injury is caused, in part, by the causative organism and, in part, by the host's own inflammatory response. In studies of experimental bacterial meningitis, adjuvant treatment with corticosteroids, specifically dexamethasone, has

  8. Endocarditis in adults with bacterial meningitis

    NARCIS (Netherlands)

    Lucas, Marjolein J.; Brouwer, Matthijs C.; van der Ende, Arie; van de Beek, Diederik


    Endocarditis may precede or complicate bacterial meningitis, but the incidence and impact of endocarditis in bacterial meningitis are unknown. We assessed the incidence and clinical characteristics of patients with meningitis and endocarditis from a nationwide cohort study of adults with

  9. Dexamethasone in adults with bacterial meningitis

    NARCIS (Netherlands)

    de Gans, Jan; van de Beek, Diederik


    Background: Mortality and morbidity rates are high among adults with acute bacterial meningitis, especially those with pneumococcal meningitis. In studies of bacterial meningitis in animals, adjuvant treatment with corticosteroids has beneficial effects. Methods: We conducted a prospective,

  10. Cognitive outcome in adults after bacterial meningitis

    NARCIS (Netherlands)

    Hoogman, Martine; van de Beek, Diederik; Weisfelt, Martijn; de Gans, Jan; Schmand, Ben


    OBJECTIVE: To evaluate cognitive outcome in adult survivors of bacterial meningitis. METHODS: Data from three prospective multicentre studies were pooled and reanalysed, involving 155 adults surviving bacterial meningitis (79 after pneumococcal and 76 after meningococcal meningitis) and 72 healthy

  11. Cognitive outcome in adults after bacterial meningitis.

    NARCIS (Netherlands)

    Hoogman, M.; Beek, D. van de; Weisfelt, M.; Gans, J. de; Schmand, B.A.


    OBJECTIVE: To evaluate cognitive outcome in adult survivors of bacterial meningitis. METHODS: Data from three prospective multicentre studies were pooled and reanalysed, involving 155 adults surviving bacterial meningitis (79 after pneumococcal and 76 after meningococcal meningitis) and 72 healthy

  12. Antimicrobial susceptibility in community-acquired bacterial ...

    African Journals Online (AJOL)

    Objectives: To determine the antimicrobial susceptibility patterns of Streptococcus pneumoniae and Haemophilus influenzae, two bacterial pathogens commonly associated with communityacquired pneumonia. Design: Cross-sectional study. Setting: Bacterial isolates were obtained from adults suspected to have ...

  13. (PCR) in the diagnosis of bacterial infections

    African Journals Online (AJOL)

    ... bacterial infections that can be diagnosed using the technique which include among others; Tuberculosis (TB), whooping cough, brain abscesses and spinal infection, otitis media with effusion, Mycoplasmal pneumonia, endophthalmitis and bacterial meningitis. Keywords: Polymerase chain reaction, Diagnosis, Bacteria, ...

  14. The Bacterial Sequential Markov Coalescent. (United States)

    De Maio, Nicola; Wilson, Daniel J


    Bacteria can exchange and acquire new genetic material from other organisms directly and via the environment. This process, known as bacterial recombination, has a strong impact on the evolution of bacteria, for example, leading to the spread of antibiotic resistance across clades and species, and to the avoidance of clonal interference. Recombination hinders phylogenetic and transmission inference because it creates patterns of substitutions (homoplasies) inconsistent with the hypothesis of a single evolutionary tree. Bacterial recombination is typically modeled as statistically akin to gene conversion in eukaryotes, i.e. , using the coalescent with gene conversion (CGC). However, this model can be very computationally demanding as it needs to account for the correlations of evolutionary histories of even distant loci. So, with the increasing popularity of whole genome sequencing, the need has emerged for a faster approach to model and simulate bacterial genome evolution. We present a new model that approximates the coalescent with gene conversion: the bacterial sequential Markov coalescent (BSMC). Our approach is based on a similar idea to the sequential Markov coalescent (SMC)-an approximation of the coalescent with crossover recombination. However, bacterial recombination poses hurdles to a sequential Markov approximation, as it leads to strong correlations and linkage disequilibrium across very distant sites in the genome. Our BSMC overcomes these difficulties, and shows a considerable reduction in computational demand compared to the exact CGC, and very similar patterns in simulated data. We implemented our BSMC model within new simulation software FastSimBac. In addition to the decreased computational demand compared to previous bacterial genome evolution simulators, FastSimBac provides more general options for evolutionary scenarios, allowing population structure with migration, speciation, population size changes, and recombination hotspots. FastSimBac is

  15. Studies of light water lattices by the homogeneous boron poisoning technique; Etude des reseaux a eau legere par la methode d'empoisonnement homogene au bore

    Energy Technology Data Exchange (ETDEWEB)

    Bailly, J.; Calament, J.; Girard, Y.; Golinelli, C. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    By homogeneously poisoning a critical facility with boric acid, one can balance important reactivities. Furthermore this technique is particularly interesting, due to the fact that it allows the substitution of cross sections to kinetic parameters in lattice calculations. With this use of boric acid as a poison arise important technological problems which are described in this report, the last part of which deals with the accuracy of the measurements made in these conditions. The main features of a lattice study made according to this moderator poisoning technique concern the determination of the concentration of dissolved boron which makes the multiplying assembly just critical and the determination of the reactivity factor as a function of temperature (this study has been carried out fill above 95 deg. C). (authors) [French] L'empoisonnement homogene d'une facilite critique par l'acide borique permet, outre d'effectuer des compensations de reactivites importantes, de jouer le role d'un intermediaire de mesure particulierement interessant par le fait qu'il conduit a substituer, dans le calcul des reseaux, des sections efficaces aux parametres cinetiques. Cette utilisation de l'acide borique comme poison ne va pas sans poser d'importants problemes technologiques qui sont decrits dans la presente etude, dont la derniere partie est consacree a la precision de mesures effectuees dans, ces conditions. Les points essentiels de l'etude d'un reseau suivant cette technique d'empoisonnement du moderateur, concernant la mesure de la concentration de bore dissous qui rend l'assemblage multiplicateur juste critique, ainsi que la determination du coefficient de reactivite en fonction de la temperature (l'etude a ete poussee au-dela de 95 deg. C). (auteurs)

  16. Bacterial reproductive pathogens of cats and dogs. (United States)

    Graham, Elizabeth M; Taylor, David J


    With the notable exception of Brucella canis, exogenous bacterial pathogens are uncommon causes of reproductive disease in cats and dogs. Most bacterial reproductive infections are endogenous, and predisposing factors for infection are important. This article reviews the etiology, pathogenesis, clinical presentation, diagnosis, treatment, and public health significance of bacterial reproductive pathogens in cats and dogs.

  17. Study of cerenkov radiation. Production of {gamma} rays by electron accelerators; Etude du rayonnement de freinage. Production de rayons {gamma} par des accelerateurs d'electrons

    Energy Technology Data Exchange (ETDEWEB)

    Bernard, J. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    This study is a critical comparison of the theories of Bremsstrahlung. Experimental results obtained by the production of {gamma} radiation with electron accelerators are compared to the theoretical results in order to estimate the extent to which the various theories are valid. (author) [French] Cette etude est une synthese des theories du rayonnement de freinage. Des resultats experimentaux, obtenus par la production de rayonnements {gamma} avec des accelerateurs d'electrons, sont compares aux resultats theoriques afin d'evaluer les domaines de validite des diverses theories. (auteur)

  18. Dynamics of bacterial gene regulation (United States)

    Narang, Atul


    The phenomenon of diauxic growth is a classical problem of bacterial gene regulation. The most well studied example of this phenomenon is the glucose-lactose diauxie, which occurs because the expression of the lac operon is strongly repressed in the presence of glucose. This repression is often explained by appealing to molecular mechanisms such as cAMP activation and inducer exclusion. I will begin by analyzing data showing that these molecular mechanisms cannot explain the strong lac repression because they exert a relatively weak effect. I will then present a minimal model accounting only for enzyme induction and dilution, which yields strong repression despite the absence of catabolite repression and inducer exclusion. The model also explains the growth patterns observed in batch and continuous cultures of various bacterial strains and substrate mixtures. The talk will conclude with a discussion of the experimental evidence regarding positive feedback, the key component of the minimal model.

  19. Bacterial cheating limits antibiotic resistance (United States)

    Xiao Chao, Hui; Yurtsev, Eugene; Datta, Manoshi; Artemova, Tanya; Gore, Jeff


    The widespread use of antibiotics has led to the evolution of resistance in bacteria. Bacteria can gain resistance to the antibiotic ampicillin by acquiring a plasmid carrying the gene beta-lactamase, which inactivates the antibiotic. This inactivation may represent a cooperative behavior, as the entire bacterial population benefits from removing the antibiotic. The cooperative nature of this growth suggests that a cheater strain---which does not contribute to breaking down the antibiotic---may be able to take advantage of cells cooperatively inactivating the antibiotic. Here we find experimentally that a ``sensitive'' bacterial strain lacking the plasmid conferring resistance can invade a population of resistant bacteria, even in antibiotic concentrations that should kill the sensitive strain. We observe stable coexistence between the two strains and find that a simple model successfully explains the behavior as a function of antibiotic concentration and cell density. We anticipate that our results will provide insight into the evolutionary origin of phenotypic diversity and cooperative behaviors.

  20. Bacterial streamers in curved microchannels (United States)

    Rusconi, Roberto; Lecuyer, Sigolene; Guglielmini, Laura; Stone, Howard


    Biofilms, generally identified as microbial communities embedded in a self-produced matrix of extracellular polymeric substances, are involved in a wide variety of health-related problems ranging from implant-associated infections to disease transmissions and dental plaque. The usual picture of these bacterial films is that they grow and develop on surfaces. However, suspended biofilm structures, or streamers, have been found in natural environments (e.g., rivers, acid mines, hydrothermal hot springs) and are always suggested to stem from a turbulent flow. We report the formation of bacterial streamers in curved microfluidic channels. By using confocal laser microscopy we are able to directly image and characterize the spatial and temporal evolution of these filamentous structures. Such streamers, which always connect the inner corners of opposite sides of the channel, are always located in the middle plane. Numerical simulations of the flow provide evidences for an underlying hydrodynamic mechanism behind the formation of the streamers.

  1. Bacterial antagonist mediated protein molecules


    Urbizu, Lucia Paola; Sparo, Mónica Delfina; Sanchez Bruni, Sergio Fabian


    Bacterial antagonism mediated by ribosomally synthesised peptides has gained considerable attention in recent years because of its potential applications in the control of undesirable microbiota. These peptides, generally referred to as bacteriocins, are defined as a heterogeneous group of ribosomally synthesised, proteinaceous substances (with or without further modifications) extracellularly secreted by many Gram-positive and some Gram-negative bacteria. Their mode of activity is primarily ...

  2. Bacterial Biofilms in Jones Tubes. (United States)

    Ahn, Eric S; Hauck, Matthew J; Kirk Harris, Jonathan; Robertson, Charles E; Dailey, Roger A

    To investigate the presence and microbiology of bacterial biofilms on Jones tubes (JTs) by direct visualization with scanning electron microscopy and polymerase chain reaction (PCR) of representative JTs, and to correlate these findings with inflammation and/or infection related to the JT. In this study, prospective case series were performed. JTs were recovered from consecutive patients presenting to clinic for routine cleaning or recurrent irritation/infection. Four tubes were processed for scanning electron microscopy alone to visualize evidence of biofilms. Two tubes underwent PCR alone for bacterial quantification. One tube was divided in half and sent for scanning electron microscopy and PCR. Symptoms related to the JTs were recorded at the time of recovery. Seven tubes were obtained. Five underwent SEM, and 3 out of 5 showed evidence of biofilms (60%). Two of the 3 biofilms demonstrated cocci and the third revealed rods. Three tubes underwent PCR. The predominant bacteria identified were Pseudomonadales (39%), Pseudomonas (16%), and Staphylococcus (14%). Three of the 7 patients (43%) reported irritation and discharge at presentation. Two symptomatic patients, whose tubes were imaged only, revealed biofilms. The third symptomatic patient's tube underwent PCR only, showing predominantly Staphylococcus (56%) and Haemophilus (36%) species. Two of the 4 asymptomatic patients also showed biofilms. All symptomatic patients improved rapidly after tube exchange and steroid antibiotic drops. Bacterial biofilms were variably present on JTs, and did not always correlate with patients' symptoms. Nevertheless, routine JT cleaning is recommended to treat and possibly prevent inflammation caused by biofilms.

  3. Bacterial sex in dental plaque

    Directory of Open Access Journals (Sweden)

    Ingar Olsen


    Full Text Available Genes are transferred between bacteria in dental plaque by transduction, conjugation, and transformation. Membrane vesicles can also provide a mechanism for horizontal gene transfer. DNA transfer is considered bacterial sex, but the transfer is not parallel to processes that we associate with sex in higher organisms. Several examples of bacterial gene transfer in the oral cavity are given in this review. How frequently this occurs in dental plaque is not clear, but evidence suggests that it affects a number of the major genera present. It has been estimated that new sequences in genomes established through horizontal gene transfer can constitute up to 30% of bacterial genomes. Gene transfer can be both inter- and intrageneric, and it can also affect transient organisms. The transferred DNA can be integrated or recombined in the recipient's chromosome or remain as an extrachromosomal inheritable element. This can make dental plaque a reservoir for antimicrobial resistance genes. The ability to transfer DNA is important for bacteria, making them better adapted to the harsh environment of the human mouth, and promoting their survival, virulence, and pathogenicity.

  4. Polymorphism in Bacterial Flagella Suspensions (United States)

    Schwenger, Walter J.

    Bacterial flagella are a type of biological polymer studied for its role in bacterial motility and the polymorphic transitions undertaken to facilitate the run and tumble behavior. The naturally rigid, helical shape of flagella gives rise to novel colloidal dynamics and material properties. This thesis studies methods in which the shape of bacterial flagella can be controlled using in vitro methods and the changes the shape of the flagella have on both single particle dynamics and bulk material properties. We observe individual flagellum in both the dilute and semidilute regimes to observe the effects of solvent condition on the shape of the filament as well as the effect the filament morphology has on reptation through a network of flagella. In addition, we present rheological measurements showing how the shape of filaments effects the bulk material properties of flagellar suspensions. We find that the individual particle dynamics in suspensions of flagella can vary with geometry from needing to reptate linearly via rotation for helical filaments to the prevention of long range diffusion for block copolymer filaments. Similarly, for bulk material properties of flagella suspensions, helical geometries show a dramatic enhancement in elasticity over straight filaments while block copolymers form an elastic gel without the aid of crosslinking agents.

  5. Detergent-compatible bacterial amylases. (United States)

    Niyonzima, Francois N; More, Sunil S


    Proteases, lipases, amylases, and cellulases are enzymes used in detergent formulation to improve the detergency. The amylases are specifically supplemented to the detergent to digest starchy stains. Most of the solid and liquid detergents that are currently manufactured contain alkaline enzymes. The advantages of using alkaline enzymes in the detergent formulation are that they aid in removing tough stains and the process is environmentally friendly since they reduce the use of toxic detergent ingredients. Amylases active at low temperature are preferred as the energy consumption gets reduced, and the whole process becomes cost-effective. Most microbial alkaline amylases are used as detergent ingredients. Various reviews report on the production, purification, characterization, and application of amylases in different industry sectors, but there is no specific review on bacterial or fungal alkaline amylases or detergent-compatible amylases. In this mini-review, an overview on the production and property studies of the detergent bacterial amylases is given, and the stability and compatibility of the alkaline bacterial amylases in the presence of the detergents and the detergent components are highlighted.

  6. Diversity of aquatic bacterial populations

    International Nuclear Information System (INIS)

    Teska, J.D.


    A study was designed to evaluate the feasibility of adapting the automated Quantum II for the identification of bacterial fish pathogens. Optimal incubation conditions were determined for each of the species used, and, by using a Chi-square goodness of fit test, it was shown that isolates could be sorted into like-species groups with a Ward's hierarchical cluster analysis technique. In a second study, population profiles, growth kinetics, and transformation kinetics were evaluated for bacteria isolated from 4 aquatic environments located in the southeastern United States. Gradual long-term accumulation of organic acids in the waters of the Okefenokee Swamp, located in southeast Georgia and northeast Florida, has resulted in acidic water ranging from pH 3.5 to 4.5. A study was designed to evaluate the metabolic efficiency of surface-water gram-negative nonfermentative bacteria and ascertain whether aquatic bacterial populations exhibit adaptation to the low pH conditions. Using the computerized AMBIS the uptake and incorporation of 35 S-methionine into bacterial proteins under 5 levels of pH was quantitated for each of the test organisms

  7. Bacterial biofilm and associated infections

    Directory of Open Access Journals (Sweden)

    Muhsin Jamal


    Full Text Available Microscopic entities, microorganisms that drastically affect human health need to be thoroughly investigated. A biofilm is an architectural colony of microorganisms, within a matrix of extracellular polymeric substance that they produce. Biofilm contains microbial cells adherent to one-another and to a static surface (living or non-living. Bacterial biofilms are usually pathogenic in nature and can cause nosocomial infections. The National Institutes of Health (NIH revealed that among all microbial and chronic infections, 65% and 80%, respectively, are associated with biofilm formation. The process of biofilm formation consists of many steps, starting with attachment to a living or non-living surface that will lead to formation of micro-colony, giving rise to three-dimensional structures and ending up, after maturation, with detachment. During formation of biofilm several species of bacteria communicate with one another, employing quorum sensing. In general, bacterial biofilms show resistance against human immune system, as well as against antibiotics. Health related concerns speak loud due to the biofilm potential to cause diseases, utilizing both device-related and non-device-related infections. In summary, the understanding of bacterial biofilm is important to manage and/or to eradicate biofilm-related diseases. The current review is, therefore, an effort to encompass the current concepts in biofilm formation and its implications in human health and disease.

  8. Dialkylresorcinols as bacterial signaling molecules. (United States)

    Brameyer, Sophie; Kresovic, Darko; Bode, Helge B; Heermann, Ralf


    It is well recognized that bacteria communicate via small diffusible molecules, a process termed quorum sensing. The best understood quorum sensing systems are those that use acylated homoserine lactones (AHLs) for communication. The prototype of those systems consists of a LuxI-like AHL synthase and a cognate LuxR receptor that detects the signal. However, many proteobacteria possess LuxR receptors, yet lack any LuxI-type synthase, and thus these receptors are referred to as LuxR orphans or solos. In addition to the well-known AHLs, little is known about the signaling molecules that are sensed by LuxR solos. Here, we describe a novel cell-cell communication system in the insect and human pathogen Photorhabdus asymbiotica. We identified the LuxR homolog PauR to sense dialkylresorcinols (DARs) and cyclohexanediones (CHDs) instead of AHLs as signals. The DarABC synthesis pathway produces the molecules, and the entire system emerged as important for virulence. Moreover, we have analyzed more than 90 different Photorhabdus strains by HPLC/MS and showed that these DARs and CHDs are specific to the human pathogen P. asymbiotica. On the basis of genomic evidence, 116 other bacterial species are putative DAR producers, among them many human pathogens. Therefore, we discuss the possibility of DARs as novel and widespread bacterial signaling molecules and show that bacterial cell-cell communication goes far beyond AHL signaling in nature.

  9. Acute bacterial meningitis in adults. (United States)

    McGill, Fiona; Heyderman, Robert S; Panagiotou, Stavros; Tunkel, Allan R; Solomon, Tom


    Over the past several decades, the incidence of bacterial meningitis in children has decreased but there remains a significant burden of disease in adults, with a mortality of up to 30%. Although the pathogenesis of bacterial meningitis is not completely understood, knowledge of bacterial invasion and entry into the CNS is improving. Clinical features alone cannot determine whether meningitis is present and analysis of cerebrospinal fluid is essential for diagnosis. Newer technologies, such as multiplex PCR, and novel diagnostic platforms that incorporate proteomics and genetic sequencing, might help provide a quicker and more accurate diagnosis. Even with appropriate antimicrobial therapy, mortality is high and so attention has focused on adjunctive therapies; adjunctive corticosteroids are beneficial in certain circumstances. Any further improvements in outcome are likely to come from either modulation of the host response or novel approaches to therapy, rather than new antibiotics. Ultimately, the best hope to reduce the disease burden is with broadly protective vaccines. Copyright © 2016 Elsevier Ltd. All rights reserved.

  10. Bacterial Carriers for Glioblastoma Therapy

    Directory of Open Access Journals (Sweden)

    Nalini Mehta


    Full Text Available Treatment of aggressive glioblastoma brain tumors is challenging, largely due to diffusion barriers preventing efficient drug dosing to tumors. To overcome these barriers, bacterial carriers that are actively motile and programmed to migrate and localize to tumor zones were designed. These carriers can induce apoptosis via hypoxia-controlled expression of a tumor suppressor protein p53 and a pro-apoptotic drug, Azurin. In a xenograft model of human glioblastoma in rats, bacterial carrier therapy conferred a significant survival benefit with 19% overall long-term survival of >100 days in treated animals relative to a median survival of 26 days in control untreated animals. Histological and proteomic analyses were performed to elucidate the safety and efficacy of these carriers, showing an absence of systemic toxicity and a restored neural environment in treated responders. In the treated non-responders, proteomic analysis revealed competing mechanisms of pro-apoptotic and drug-resistant activity. This bacterial carrier opens a versatile avenue to overcome diffusion barriers in glioblastoma by virtue of its active motility in extracellular space and can lead to tailored therapies via tumor-specific expression of tumoricidal proteins.

  11. Análisis de la degeneración de los fotorreceptoes en modelos experimentales de retinosis pigmentaria, degeneración macular asociada a la edad y glaucoma =Analysis of photoreceptor degeneration in experimental models of retinitis pigmentosa, aging macular degeneration and glaucoma


    Ortín Martínez, Arturo


    Objetivo. Determinar el número total y la topografía de la población de conos en roedores adultos utilizando rutinas automatizadas que nos permita investigar objetivamente los efectos de diferentes modelos experimentales de patologías humanas tales como la degeneración macular asociada a la edad (DMAE), retinosis pigmentaria (RP) y la neuropatía óptica glaucomatosa (NOG) sobre la población de fotorreceptores. Material y métodos. Se han utilizado cinco cepas distinta de roedores: Sprague-Dawle...

  12. Análisis de la degeneración de los fotorreceptoes en modelos experimentales de retinosis pigmentaria, degeneración macular asociada a la edad y glaucoma =Analysis of photoreceptor degeneration in experimental models of retinitis pigmentosa, aging macular degeneration and glaucoma


    Ortín Martínez, Arturo


    . Objetivo. Determinar el número total y la topografía de la población de conos en roedores adultos utilizando rutinas automatizadas que nos permita investigar objetivamente los efectos de diferentes modelos experimentales de patologías humanas tales como la degeneración macular asociada a la edad (DMAE), retinosis pigmentaria (RP) y la neuropatía óptica glaucomatosa (NOG) sobre la población de fotorreceptores. Material y métodos. Se han utilizado cinco cepas distinta de roedor...

  13. Research trends in radiobiology since 40 years. a new approach: the enzymatic repair function of DNA, internal factor in evolution of biological systems under irradiation; Etude des tendances des recherches en radiologie depuis 40 ans. Une nouvelle voie de recherche: la fonction de reparation enzymatique de l'ADN, facteur interne d'evolution des systemes biologiques sous rayonnement

    Energy Technology Data Exchange (ETDEWEB)

    Mouton, R. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    . Il y a moins d'une generation, l'on croyait encore que les effets des rayonnements sur les systemes vivants etaient des effets directs, immediats, irreparables et definitifs. Depuis lors, on s'est rendu compte que les radiolesions pouvaient resulter d'effets indirects, etre tardives, reparables, et le plus souvent modifiables par des traitements chimiques ou biochimiques ad hoc. Cependant, la percee decisive en radiobiologie se situe entre 1962 et 1964, avec la decouverte que la cellule possede un mecanisme de defense active vis-a-vis de tout ce qui peut porter atteinte a l'integrite du patrimoine genetique inscrit dans la structure meme de l'ADN. L'existence d'une quatrieme fonction de l'ADN, celle d'auto-reparation, par voie enzymatique sous controle genetique, apporte enfin a la radiobiologie la base biologique moleculaire qui lui manquait pour sortir de la nuit 'phenomenologique' ou elle s'etait enfoncee apres l'abandon de la generalisation de la theorie de Lea, faute de preuve experimentale. Dans la seconde partie, cette fois prospective, de l'etude l'auteur tente une synthese elargie en considerant le role que peut jouer cette fonction de reparation de l'ADN non seulement du point de vue de la survie des systemes biologiques irradies en presence ou non d'agents modificateurs de dose ou mutagenes, mais aussi de celui de l'evolution naturelle ou artificielle de ces systemes sous rayonnement. Les donnees les plus recentes de la litterature sont en accord avec ce qui ne constitue encore qu'une hypothese generale de recherche. Des travaux portant sur les caracteres phenotypiques et genotypiques lies a l'acquisition de radioresistance gamma et UV, chez 'Escherichia coli K12', ont ete entrepris par l'auteur, en collaboration avec O. Tremeau en vue d'apporter une contribution experimentale nouvelle a cet egard. (auteur)

  14. Contribution to the micro-calorimetric study of the characteristics of radio-elements and to their qualitative and quantitative analysis; Contribution a l'etude microcalorimetrique des caracteristiques des radio-elements et a leurs analyses qualitative et quantitative

    Energy Technology Data Exchange (ETDEWEB)

    Ozias, Y. [Centre de Production de Plutonium de Marcoule, 30 (France)


    accuracy equal to that obtained by mass spectrography (destruction of the sample) 1 to 3 per cent, and higher than that obtained by counting techniques (greater than 5 per cent). (author) [French] Le principe de cette etude consiste a enregistrer avec le microcalorimetre E. CALVET le flux d'energie emis par les radioelements en fonction du temps at a partir de cette courbe de determiner soit leurs caracteristiques (periode et energie du rayonnement), soit la composition d'un melange de radioelements. Dans une premiere partie on a adapte les relations theoriques de la radioactivite a l'etude microcalorimetrique. Dans une deuxieme partie on determine experimentalement au microcalorimetre E. CALVET les caracteristiques de divers radioelements, yttrium 90, phosphore 32, soufre 35, iode 131, etc. et leurs melanges. On a etudie plus particulierement l'analyse isotopique du melange de {sup 239}Pu, de {sup 240}Pu et eventuellement de {sup 241}Pu. La methode microcalorimetrique n'a pas la sensibilite des methodes classiques de comptage, sa limite actuelle para etre de l'ordre du micro-watt pour les appareils utilises, mais elle est la plus directe pour les emetteurs de rayonnements facilement absorbables({alpha} et {beta}). Pour les emetteurs {gamma} une partie seulement de l'energie est absorbee par les parois du recipient. La determination de leurs caracteristiques et de leurs masses dans les melanges ne depend ni de la forme, ni de la dilution, ni surtout de l'auto-absorption de la source. Pour des echantillons de forte activite, cette auto-absorption est pour les methodes classiques la source de grosses difficultes entra ant de multiples manipulations: divisions, mise en solution, dilutions qui introduisent autant de causes d'erreur. La methode calorimetrique permet de travailler sur l'echantillon brut. Toutes les mesures ont ete faites avec le microcalorimetre E. CALVET classique; mais pour effectuer une mesure tres precise de

  15. Serotonin transporter gene polymorphism and myocardial infarction: Etude Cas-Témoins de l'Infarctus du Myocarde (ECTIM). (United States)

    Fumeron, Frédéric; Betoulle, Dina; Nicaud, Viviane; Evans, Alun; Kee, Frank; Ruidavets, Jean-Bernard; Arveiler, Dominique; Luc, Gérald; Cambien, François


    Depression is a risk factor for myocardial infarction (MI). Selective serotonin reuptake inhibitors reduce this risk. The site of action is the serotonin transporter (SLC6A4), which is expressed in brain and blood cells. A functional polymorphism in the promoter region of the SLC6A4 gene has been described. This polymorphism may be associated with the risk of MI. The SLC6A4 polymorphism has been investigated by polymerase chain reaction in 671 male patients with MI and in 688 controls from the Etude Cas-Témoins de l'Infarctus du Myocarde (ECTIM) multicentric study. Percentages for LL, LS, and SS genotypes were 35.5%, 45.4%, and 19.1%, respectively, for cases versus 28.1%, 49.1%, and 22.8%, respectively, for controls. S allele frequency was 41.8% and 47.4% for cases and controls, respectively. After adjustment for age and center by using multivariable logistic regression, the odds ratio for MI associated with the LL genotype was 1.40 (95% CI 1.11 to 1.76, P=0.0047). The LL genotype of the SLC6A4 polymorphism is associated with a higher risk of MI. This could be attributable to the effect of the polymorphism on serotonin-mediated platelet activation or smooth muscle cell proliferation or on other risk factors, such as depression or response to stress.

  16. Cognitive decline is associated with systemic oxidative stress: the EVA study. Etude du Vieillissement Artériel. (United States)

    Berr, C; Balansard, B; Arnaud, J; Roussel, A M; Alpérovitch, A


    To determine whether systemic oxidative stress status is associated with cognitive decline. A longitudinal population-based study. A cohort study of older subjects in Nantes, France. A total of 1166 high cognitive functioning subjects aged 60 to 70 in the Etude du Vieillissement Arteriel (EVA) cohort with a 4 year follow-up. Subjects completed a baseline interview and a global cognitive test (Mini-Mental Status Examination (MMSE)). Blood samples were obtained at baseline to determine plasma levels of selenium, carotenoids, thiobarbituric acid reactant substances (TBARS), an indicator of lipoperoxidation, and red blood cell vitamin E. Risk of cognitive decline, defined as a loss of 3 points in MMSE score between baseline and the 4 year follow-up, was assessed by oxidative stress level. Subjects with the highest levels of TBARS show an increased risk of cognitive decline (adjusted odds ratio (OR) = 2.25; confidence interval (CI) 95% = 1.26-4.02). This result is reinforced in the lower antioxidant status subgroup. Subjects with low levels of selenium have an increased risk of cognitive decline (OR = 1.58; CI 95% = 1.08-2.31) after adjustment for various confounding factors. These results suggest that increased levels of oxidative stress and/or antioxidant deficiencies may pose risk factors for cognitive decline. The direct implication of oxidative stress in vascular and neurodegenerative mechanisms that lead to cognitive impairment should be further explored.

  17. Etudes sur l'histoire de la pauvreté (Moyen Age — XVIe siècle

    Directory of Open Access Journals (Sweden)

    Hilário Franco Júnior


    Full Text Available MOLLAT, Michel (dir..  Etudes sur l'histoire de la pauvreté (Moyen Age — XVIe siècle. 2 vols. Paris Publications de la Sorbonne, 1974. 855p. (primeiro parágrafo do artigo Esta obra reúne alguns dos trabalhos desenvolvidos nos seminários sobre pobres e pobreza na Idade Média, que o professor Michel Mollat coordena desde 1962 na Sorbonne. Após um rápido e excelente ensaio introdutório, assinado pelo organizador da obra, no qual se colocam alguns dos problemas relativos ao estudo da pobreza medieval, seguem-se 35 estudos, devidos a diferentes colaboradores. O primeiro volume, correspondente à primeira parte da obra ("Pobreza e caridade, valores individuais" agrupa 21 estudos, e o segundo ("Desenvolvimento da pobreza e organização da assistência". Uma bibliografia de cerca de 160 títulos, uma lista de palavras-chaves latinas de uso mais comum na documentação da Idade Média e algumas gravuras extra-texto de boa qualidade completam o livro.

  18. Contribution a l'etude et au developpement de nouvelles poudres de fonte (United States)

    Boisvert, Mathieu

    " uphill " permet de conserver plus ou moins de graphite nodulaire a l'interieur des regions riches en silicium, alors que le reste du graphite precipite sous forme lamellaire/vermiculaire dans les regions interparticulaires. L'etude des poudres de fontes et la recherche des mecanismes regissant la morphologie du graphite dans les fontes coulees/moulees nous ont amenes a produire des poudres de fontes traitees au magnesium avant l'atomisation. Plusieurs resultats fondamentaux ont ete obtenus de la caracterisation des poudres traitees au magnesium et de leur comparaison avec des poudres de chimies similaires non traitees au magnesium. D'abord, il a ete possible d'observer des bifilms d'oxyde de silicium dans la structure du graphite primaire d'une poudre de fonte grise hypereutectique. Il s'agit des premieres images montrant la structure double de ces defauts, venant ainsi appuyer la theorie elaboree par le professeur John Campbell. Ensuite, il a ete montre que le traitement au magnesium forme une atmosphere protectrice gazeuse autogeneree qui empeche l'oxydation de la surface du bain liquide et donc, la formation et l'entrainement des bifilms. Le role du magnesium sur la morphologie du graphite est de diminuer le soufre en solution en formant des precipites de sulfure de magnesium et ainsi d'augmenter l'energie d'interface graphite-liquide. En reponse a cette grande energie d'interface graphite-liquide, le graphite cherche a minimiser son ratio surface/volume, ce qui favorise la formation de graphite spheroidal. De plus, deux types de germination ont ete observes dans la poudre de fonte hypereutectique traitee au magnesium. Le premier type est une germination heterogene sur un nombre limite de particules faites de magnesium, d'aluminium, de silicium et d'oxygene. Le deuxieme type est une germination homogene des nodules dans certaines regions du liquide plus riches en silicium. L'observation du centre reel tridimensionnel, en microscopie electronique en transmission en

  19. Etude des phenomenes dynamiques ultrarapides et des caracteristiques impulsionnelles d'emission terahertz du supraconducteur YBCO (United States)

    Savard, Stephane

    Les premieres etudes d'antennes a base de supraconducteurs a haute temperature critique emettant une impulsion electromagnetique dont le contenu en frequence se situe dans le domaine terahertz remontent a 1996. Une antenne supraconductrice est formee d'un micro-pont d'une couche mince supraconductrice sur lequel un courant continu est applique. Un faisceau laser dans le visible est focalise sur le micro-pont et place le supraconducteur dans un etat hors-equilibre ou des paires sont brisees. Grace a la relaxation des quasiparticules en surplus et eventuellement de la reformation des paires supraconductrices, nous pouvons etudier la nature de la supraconductivite. L'analyse de la cinetique temporelle du champ electromagnetique emis par une telle antenne terahertz supraconductrice s'est averee utile pour decrire qualitativement les caracteristiques de celle-ci en fonction des parametres d'operation tels que le courant applique, la temperature et la puissance d'excitation. La comprehension de l'etat hors-equilibre est la cle pour comprendre le fonctionnement des antennes terahertz supraconductrices a haute temperature critique. Dans le but de comprendre ultimement cet etat hors-equilibre, nous avions besoin d'une methode et d'un modele pour extraire de facon plus systematique les proprietes intrinseques du materiau qui compose l'antenne terahertz a partir des caracteristiques d'emission de celle-ci. Nous avons developpe une procedure pour calibrer le spectrometre dans le domaine temporel en utilisant des antennes terahertz de GaAs bombarde aux protons H+ comme emetteur et detecteur. Une fois le montage calibre, nous y avons insere une antenne emettrice dipolaire de YBa 2Cu3O7-delta . Un modele avec des fonctions exponentielles de montee et de descente du signal est utilise pour lisser le spectre du champ electromagnetique de l'antenne de YBa 2Cu3O7-delta, ce qui nous permet d'extraire les proprietes intrinseques de ce dernier. Pour confirmer la validite du modele

  20. ESTUDIO DE SECUENCIAS DE TALLA LÍTICA A TRAVÉS DE MODELOS EXPERIMENTALES EN ROCAS SILÍCEAS DEL CENTRO DE ARGENTINA (Study of lithic carving sequences through experimental models in siliceous rocks of central Argentina

    Directory of Open Access Journals (Sweden)

    Eduardo Pautassi


    Full Text Available La evidencia arqueológica dejada por los grupos humanos que habitaron el centro de Argentina (provincias de Córdoba y San Luis, desde las primeras ocupaciones hasta momentos previos a la conquista española, pone de manifiesto que estas poblaciones desarrollaron diversas estrategias y adaptaciones a lo largo del tiempo. Una de estas estrategias es la producción de artefactos líticos. En este trabajo, se aborda el estudio de las secuencias de reducción de clastos y de manufactura de bifaces experimentales aplicando la metodología de análisis «no tipológico» para el estudio de los desechos de talla. Estas experiencias fueron realizadas como resultado del análisis de la tecnología lítica de sitios arqueológicos ubicados en la localidad arqueológica de Estancia La Suiza, San Luis. La finalidad de este trabajo es comparar ciertos atributos de las lascas producto de la experimentación para diferenciar tipos de actividades de talla. Realizar esta propuesta nos permite presentar en otra oportunidad la comparación con el registro arqueológico, e intentar dilucidar cómo fueron los diferentes momentos en el proceso de talla. ENGLISH: The archaeological evidence left by the humans who occupied the center of Argentina (comprising the present territory of the provinces of Cordoba and San Luis, Argentina, from the earliest settlements until the moments before the Spanish conquest, shows that these populations developed different strategies and adaptations to the environment over time. One such strategy is the production of lithic artifacts. This contribution specifically addresses the study of sequence of cores and reducing manufacturing bifaces by applying the methodology of the “non-typological” analysis to the study of debitage. These experiments were conducted as a result of the analysis of the lithic technology of archaeological sites located at the archaeological locality of Estancia La Suiza, in San Luis province. The purpose of

  1. Etude de la réplication de l'ADN chez les Archaea


    Berthon , Jonathan


    Thèse réalisée entre France et Japon (boursier JSPS); Cellular organisms belong to one of the three domains of life: Archaea, Bacteria, and Eucarya. Archaea are unicellular organisms with a bacterial phenotype, yet they exhibit many eucaryotic features at the molecular level. In particular, archaeal DNA replication machinery is a homologous and simplified version of that in eucaryotes. In this work, I have studied archaeal DNA replication with both in vitro and in silico approaches.First, I h...

  2. [Requests for emergency appointments with dermatologists in private practice in France: the ETude Urgences en DErmatologie libérale (ET.U.DE) study]. (United States)

    Penso-Assathiany, D; Bourdon-Lanoy, E; Derancourt, C; Roujeau, J-C; Bastuji-Garin, S


    Requests for emergency appointments are fairly common in private dermatologic practice in France. To our knowledge the frequency and reasons for such requests have not yet been evaluated. The primary objective of our study was to provide a quantitative and qualitative evaluation of such requests and to assess the underlying reasons. The secondary aim was to investigate for an association between emergency requests where response was justified within 48 hours and the symptoms reported by patients in order to establish a predictive score for the validity of requests. The study took place during one week in April 2004. Forty French dermatologists took part on a voluntary basis. They deliberately reduced their ongoing schedule to accommodate patients seeking an urgent appointment. We collected data regarding the normal professional activity of each dermatologist, reasons for appointments, symptoms, diagnosis and evaluation of the degree of emergency by the attending dermatologist. Univariate and multivariate analyses were performed and a score was attributed based on the results for the variables used in the logistic regression model. The mean number of patients seen at emergency appointments during the study week doubled in comparison with a normal period. During the week, 613 patients phoned and all questionnaires were completed for 538 (88%) patients. The most common reasons for requesting an appointment were: rash, eczema, pruritus, tumour modification, localized lesion and allergy. The most frequent diagnoses were: eczema, bacterial and viral infection, atopic dermatitis, mycosis and naevus. The dermatologists considered that the consultations were justified within 48 hours for one third of patients. In the multivariate analysis, factors significantly associated with a justified request were of a general medical rather than a dermatological nature: enlarged cysts, blisters, insomnia, impaired activity, onset or aggravation within the previous 7 days, inability to

  3. Analysis and exploitation of bacterial population from natural uranium-rich soils: selection of a model specie; Analyse et exploitation des populations bacteriennes de sols riches en uranium: selection d'une espece modele

    Energy Technology Data Exchange (ETDEWEB)

    Mondani, L.


    It is well known that soils play a key role in controlling the mobility of toxic metals and this property is greatly influenced by indigenous bacterial communities. This study has been conducted on radioactive and controls soils, collected in natural uraniferous areas (Limousin). A physico-chemical and mineralogical analysis of soils samples was carried out.The structure of bacterial communities was estimated by Denaturing Gradient Gel Electrophoresis (DGGE). The community structure is remarkably more stable in the uranium-rich soils than in the control ones, indicating that uranium exerts a high selection from the soils was constructed and screened for uranium resistance in order to study bacteria-uranium interactions. Scanning electron microscopy revealed that a phylo-genetically diverse set of uranium-resistant species ware able to chelate uranium at the cell surface. (author) [French] On sait que les sols et les populations bacteriennes indigenes ont une influence sur la mobilite des metaux, donc sur leur toxicite. Cette etude a ete menee sur des sols uraniferes et controles collectes dans le Limousin (regions naturellement riches en uranium ). Une analyse physico-chimique et mineralogique des echantillons de sol a ete realisee. La structure des communautes bacteriennes a ete etudiee par electrophorese en gradient de denaturant (DGGE). La structure des communautes est remarquablement stable dans les sols uraniferes, ce qui indique que l'uranium exerce une forte pression de selection. D'autre part, une collection de bacteries cultivables a ete realisee a partir des sols, puis criblee pour la resistance a l'uranium, dans le but d'etudier les interactions entre bacteries et uranium. Des observations en Microscopie electronique a Balayage ont mis en evidence differents mecanismes de chelation de l'uranium a la surface cellulaire

  4. Molecular Basis of Bacterial Longevity

    Directory of Open Access Journals (Sweden)

    Kieran B. Pechter


    Full Text Available It is well known that many bacteria can survive in a growth-arrested state for long periods of time, on the order of months or even years, without forming dormant structures like spores or cysts. How is such longevity possible? What is the molecular basis of such longevity? Here we used the Gram-negative phototrophic alphaproteobacterium Rhodopseudomonas palustris to identify molecular determinants of bacterial longevity. R. palustris maintained viability for over a month after growth arrest due to nutrient depletion when it was provided with light as a source of energy. In transposon sequencing (Tn-seq experiments, we identified 117 genes that were required for long-term viability of nongrowing R. palustris cells. Genes in this longevity gene set are annotated to play roles in a number of cellular processes, including DNA repair, tRNA modification, and the fidelity of protein synthesis. These genes are critically important only when cells are not growing. Three genes annotated to affect translation or posttranslational modifications were validated as bona fide longevity genes by mutagenesis and complementation experiments. These genes and others in the longevity gene set are broadly conserved in bacteria. This raises the possibility that it will be possible to define a core set of longevity genes common to many bacterial species.

  5. Phenotypic signatures arising from unbalanced bacterial growth. (United States)

    Tan, Cheemeng; Smith, Robert Phillip; Tsai, Ming-Chi; Schwartz, Russell; You, Lingchong


    Fluctuations in the growth rate of a bacterial culture during unbalanced growth are generally considered undesirable in quantitative studies of bacterial physiology. Under well-controlled experimental conditions, however, these fluctuations are not random but instead reflect the interplay between intra-cellular networks underlying bacterial growth and the growth environment. Therefore, these fluctuations could be considered quantitative phenotypes of the bacteria under a specific growth condition. Here, we present a method to identify "phenotypic signatures" by time-frequency analysis of unbalanced growth curves measured with high temporal resolution. The signatures are then applied to differentiate amongst different bacterial strains or the same strain under different growth conditions, and to identify the essential architecture of the gene network underlying the observed growth dynamics. Our method has implications for both basic understanding of bacterial physiology and for the classification of bacterial strains.

  6. Interaction between resource identity and bacterial community composition regulates bacterial respiration in aquatic ecosystems

    Directory of Open Access Journals (Sweden)

    A. P. F. Pires

    Full Text Available Abstract Resource identity and composition structure bacterial community, which in turn determines the magnitude of bacterial processes and ecological services. However, the complex interaction between resource identity and bacterial community composition (BCC has been poorly understood so far. Using aquatic microcosms, we tested whether and how resource identity interacts with BCC in regulating bacterial respiration and bacterial functional diversity. Different aquatic macrophyte leachates were used as different carbon resources while BCC was manipulated through successional changes of bacterial populations in batch cultures. We observed that the same BCC treatment respired differently on each carbon resource; these resources also supported different amounts of bacterial functional diversity. There was no clear linear pattern of bacterial respiration in relation to time succession of bacterial communities in all leachates, i.e. differences on bacterial respiration between different BCC were rather idiosyncratic. Resource identity regulated the magnitude of respiration of each BCC, e.g. Ultricularia foliosa leachate sustained the greatest bacterial functional diversity and lowest rates of bacterial respiration in all BCC. We conclude that both resource identity and the BCC interact affecting the pattern and the magnitude of bacterial respiration in aquatic ecosystems.

  7. Determination experimentale et approches simplifies de ...

    African Journals Online (AJOL)

    Les caractéristiques thermiques, physiques voire mécaniques de l'espèce de bois étudiée et la durabilité du matériau dans les constructions traditionnelles à partir du Borassus permettent d'envisager son utilisation comme armature dans le béton. Mots clés : Borassus Aethiopum (palmier rônier), transfert de chaleur, ...

  8. Estudios experimentales de cuatro Rauvolfias colombianas

    Directory of Open Access Journals (Sweden)

    Enrique Núñez Olarte


    Full Text Available Four species of Colombian rauwolfias, R. tetraphylla L., R. littoralis rusby, R. ligustrina R. y S., and R. leptophylla A. s. rap, were studied in order to identify, determine and evaluate the toxicity of their alkaloids and their application in pharmacodynamics. A comparison of the action of these drugs with reserpine was made and some methods were devised to verify their hypotensive, tranquilizing and ulcerogenic properties.

  9. Diagnosis and prevalence of bacterial vaginosis.

    Directory of Open Access Journals (Sweden)

    Saharan S


    Full Text Available A prospective study of 80 women was undertaken to estimate the prevalence of bacterial vaginosis, and to compare two methods of diagnosing the condition. Bacterial Vaginosis was detected by both Gram stain and compound criteria in 30 women. The prevalence was 37.5%. Gram stain provides a simple and inexpensive method for laboratory confirmation of bacterial vaginosis where facilities for using the compound criteria are not available.

  10. Balance of bacterial species in the gut

    Indian Academy of Sciences (India)

    First page Back Continue Last page Overview Graphics. Balance of bacterial species in the gut. Protective. Lactobacillus species. Bifidobacterium species. Selected E. coli. Saccharomyces boulardii. Clostridium butyricum.

  11. Etude energetique et modelisation d'un Systeme Hybride Eolien-Diesel avec stockage d'Air Comprime (SHEDAC) (United States)

    Martinez, Nicolas

    Actuellement le Canada et plus specialement le Quebec, comptent une multitude de sites isoles dont !'electrification est assuree essentiellement par des generatrices diesel a cause, principalement, de l'eloignement du reseau central de disuibution electrique. Bien que consideree comme une source fiable et continue, 1 'utilisation des generatrices diesel devient de plus en plus problematique d'un point de vue energetique, economique et environnemental. Dans le but d'y resoudre et de proposer une methode d'approvisionnement plus performante, mains onereuse et plus respectueuse de l'environnement, l'usage des energies renouvelables est devenu indispensable. Differents travaux ont alors demontre que le couplage de ces energies avec des generauices diesel, formant des systemes hybrides, semble etre une des meilleures solutions. Parmi elles, le systeme hybride eolien-diesel avec stockage par air comprime (SHEDAC) se presente comme une configuration optimale pour 1 'electrification des sites isoles. En effet, differentes etudes ont permis de mettre en avant l'efficacite du stockage par air comprime, par rapport a d'autres technologies de stockage, en complement d'un systeme hybride eolien-diesel. Plus precisement, ce systeme se compose de sous-systemes qui sont: des eoliennes, des generatrices diesel et une chaine de compression et de stockage d'air comprime qui est ensuite utilisee pour suralimenter les generatrices. Ce processus permet ainsi de reduire Ia consommation en carburant tout en agrandissant la part d'energie renouvelable dans Ia production electrique. A ce jour, divers travaux de recherche ont pennis de demontrer I' efficacite d 'un tel systeme et de presenter une variete de configurations possibles. A travers ce memoire, un logiciel de dimensionnement energetique y est elabore dans le but d'unifom1iser !'approche energetique de cette technologie. Cet outil se veut etre une innovation dans le domaine puisqu'il est actuellement impossible de dimensionner un

  12. Composes inter-halogenes sous pression: etude des transformations structurales dans le monobromure d'iode sous forme dense (United States)

    Bouchard, Alexandre

    La famille des composes halogenes et inter-halogenes representent des solides moleculaires adoptant des phases denses communes avec des solides moleculaires diatomiques comme l'azote et l'hydrogene. Parmi les transformations structurales et electroniques induites sous haute pression et observees dans ces solides, on note, entre autres, la dissociation moleculaire et la metallisation. De plus, l'etude des phases denses de l'iode a permis recemment l'observation d'une structure cristalline possedant une modulation dite incommensurable, c'est-a-dire une modulation possedant une periodicite differente de celle de la structure cristalline, jetant ainsi une lumiere nouvelle sur le processus de dissociation moleculaire dans les solides halogenes. Dans ce memoire, on propose d'etudier les changements structuraux dans monobromure d'iode (IBr), un compose inter-halogene possedant des proprietes structurales semblables a celles de deux composes halogenes, soit l'iode (I 2) et le brome (Br2) sous leur forme solide. Des experiences de diffraction des rayons X de poudres en utilisant un rayonnement synchrotron ont ete realisees a temperature ambiante sur l'IBr en variant la pression jusqu'aux environs de 60 GPa. La nature chimique particuliere du compose IBr a necessite la mise au point de techniques de chargement d'echantillon destinees a preserver l'integrite chimique de la substance utilisee. On rapporte egalement l'observation d'une phase de l'IBr presentant une modulation incommensurable. Les phases observees dans l'IBr permettent d'etablir des paralleles avec les phases denses rapportees dans I2 et Br2 par le biais d'un modele phenomenologique decrivant la sequence structurale des solides halogenes sous forme condensee.

  13. Systematic study of plasma and serum proteins in the pig; Etude systematique des proteines plasmatiques et seriques du porc

    Energy Technology Data Exchange (ETDEWEB)

    Daburon, F.; Nizza, P.; Hatchikian, C.; Schmidt, J.-P. [Commissariat a l' Energie Atomique (France)


    This work has been carried out in the framework of the determination of the physiological constants of a normal pig. The aim was to study the serum and plasma proteins of this animal species, the ultimate object being to discover whether the qualitative and quantitative changes in these proteins can make a significant contribution to the establishment of a biological dosimetry for irradiated pigs. The serum and plasma from a normal pig were analyzed first by various simple electrophoretic methods and then by immuno-electrophoresis. As a result of the particular characteristics of pig serum we have gradually been led to make numerous modifications to the techniques used for human serums or for those of small laboratory animals. Much careful work and patience were required in order to obtain reproducible results. (authors) [French] Ce travail se situe dans le cadre de la determination des constantes physiologiques du porc normal. il s'agissait de proceder a l'etude des proteines seriques et plasmatiques de cette espece animale, le but ulterieur etant de savoir si les modifications qualitatives et quantitatives de ces proteines pourront representer une contribution valable a l'etablissement d'une dosimetrie biologique chez le porc irradie. Le serum et le plasma du porc normal ont ete analyses d'abord par diverses methodes electrophoretiques simples puis par immunoelectrophorese. Les caracteristiques particulieres du serum de porc nous ont conduits a apporter progressivement de nombreuses modifications aux techniques utilisees pour des serums humains ou de petits animaux de laboratoire. L'obtention de resultats reproductible a exige beaucoup de patience et de minutie. (auteurs)

  14. Flexible Hinges in Bacterial Chemoreceptors. (United States)

    Akkaladevi, Narahari; Bunyak, Filiz; Stalla, David; White, Tommi A; Hazelbauer, Gerald L


    Transmembrane bacterial chemoreceptors are extended, rod-shaped homodimers with ligand-binding sites at one end and interaction sites for signaling complex formation and histidine kinase control at the other. There are atomic-resolution structures of chemoreceptor fragments but not of intact, membrane-inserted receptors. Electron tomography of in vivo signaling complex arrays lack distinct densities for chemoreceptor rods away from the well-ordered base plate region, implying structural heterogeneity. We used negative staining, transmission electron microscopy, and image analysis to characterize the molecular shapes of intact homodimers of the Escherichia coli aspartate receptor Tar rendered functional by insertion into nanodisc-provided E. coli lipid bilayers. Single-particle analysis plus tomography of particles in a three-dimensional matrix revealed two bend loci in the chemoreceptor cytoplasmic domain, (i) a short, two-strand gap between the membrane-proximal, four-helix-bundle HAMP (histidine kinases, adenylyl cyclases, methyl-accepting chemoreceptors, and phosphatases) domain and the membrane-distal, four-helix coiled coil and (ii) aligned glycines in the extended, four-helix coiled coil, the position of a bend noted in the previous X-ray structure of a receptor fragment. Our images showed HAMP bends from 0° to ∼13° and glycine bends from 0° to ∼20°, suggesting that the loci are flexible hinges. Variable hinge bending explains indistinct densities for receptor rods outside the base plate region in subvolume averages of chemotaxis arrays. Bending at flexible hinges was not correlated with the chemoreceptor signaling state. However, our analyses showed that chemoreceptor bending avoided what would otherwise be steric clashes between neighboring receptors that would block the formation of core signaling complexes and chemoreceptor arrays. IMPORTANCE This work provides new information about the shape of transmembrane bacterial chemoreceptors, crucial

  15. Metabolic signatures of bacterial vaginosis. (United States)

    Srinivasan, Sujatha; Morgan, Martin T; Fiedler, Tina L; Djukovic, Danijel; Hoffman, Noah G; Raftery, Daniel; Marrazzo, Jeanne M; Fredricks, David N


    Bacterial vaginosis (BV) is characterized by shifts in the vaginal microbiota from Lactobacillus dominant to a microbiota with diverse anaerobic bacteria. Few studies have linked specific metabolites with bacteria found in the human vagina. Here, we report dramatic differences in metabolite compositions and concentrations associated with BV using a global metabolomics approach. We further validated important metabolites using samples from a second cohort of women and a different platform to measure metabolites. In the primary study, we compared metabolite profiles in cervicovaginal lavage fluid from 40 women with BV and 20 women without BV. Vaginal bacterial representation was determined using broad-range PCR with pyrosequencing and concentrations of bacteria by quantitative PCR. We detected 279 named biochemicals; levels of 62% of metabolites were significantly different in women with BV. Unsupervised clustering of metabolites separated women with and without BV. Women with BV have metabolite profiles marked by lower concentrations of amino acids and dipeptides, concomitant with higher levels of amino acid catabolites and polyamines. Higher levels of the signaling eicosanoid 12-hydroxyeicosatetraenoic acid (12-HETE), a biomarker for inflammation, were noted in BV. Lactobacillus crispatus and Lactobacillus jensenii exhibited similar metabolite correlation patterns, which were distinct from correlation patterns exhibited by BV-associated bacteria. Several metabolites were significantly associated with clinical signs and symptoms (Amsel criteria) used to diagnose BV, and no metabolite was associated with all four clinical criteria. BV has strong metabolic signatures across multiple metabolic pathways, and these signatures are associated with the presence and concentrations of particular bacteria. Bacterial vaginosis (BV) is a common but highly enigmatic condition that is associated with adverse outcomes for women and their neonates. Small molecule metabolites in the

  16. Musculoskeletal manifestations of bacterial endocarditis

    Directory of Open Access Journals (Sweden)

    Érika Bevilaqua Rangel


    Full Text Available CONTEXT: The incidence of staphylococcal infection has been increasing during the last 20 years. OBJECTIVE: Report a case of staphylococcal endocarditis preceded by musculoskeletal manifestations, which is a rare form of clinical presentation. DESIGN: Case report. CASE REPORT: A 45-year-old-man, without addictions and without known previous cardiopathy, was diagnosed as having definitive acute bacterial endocarditis due to Staphylococcus aureus. Its etiology was community-acquired, arising from a non-apparent primary focus. In addition, the musculoskeletal symptoms preceded the infective endocarditis (IE by about 1 month, which occurred together with other symptoms, e.g. mycotic aneurysms and petechiae. Later, the patient showed perforation of the mitral valve and moderate mitral insufficiency with clinical control.

  17. Bacterial biofilms and antibiotic resistance

    Directory of Open Access Journals (Sweden)

    Liliana Caldas-Arias


    Full Text Available Biofilms give to bacteria micro-environmental benefits; confers protection against antimicrobials. Bacteria have antibiotic resistance by conventional and unusual mechanisms leading to delayed wound healing, to increase recurrent chronic infections and nosocomial contamination of medical devices. Objective: This narrative review aims to introduce the characteristics of Bacteria-biofilms, antimicrobial resistance mechanisms and potential alternatives for prevention and control of its formation. Methods: Search strategy was performed on records: PubMed / Medline, Lilacs, Redalyc; with suppliers such as EBSCO and thesaurus MeSH and DeCS. Conclusions: Knowledge and research performance of biofilm bacteria are relevant in the search of technology for detection and measuring sensitivity to antibiotics. The identification of Bacterial-biofilms needs no-traditional microbiological diagnosis.

  18. Microconductometric Detection of Bacterial Contamination

    Directory of Open Access Journals (Sweden)

    Sarra EL ICHI


    Full Text Available Several approaches can be used for the electrochemical detection of bacterial contamination. Their performance can be assessed by the ability to detect bacteria at very low concentrations within a short-time response. We have already demonstrated that a conductometric biosensor based on interdigitated thin-film electrodes is adapted to detect bacteria in clinical samples like serum and compatible with microfluidic fabrication. The type of interdigitated microelectrodes influences the performance of the biosensor. This was shown by the results obtained in this work. A magnetic-nanoparticles based immunosensor was designed using gold screen-printed electrodes. The immunosensor was able to specifically detect E. coli in the range of 1-103 CFU mL-1. The new transducer offered a larger active sensing surface with a lower cost and a robust material. Accuracy of the conductance value was enhanced by differential measurements. The immunosensor is compatible with a microfluidic system.

  19. Overview perspective of bacterial resistance. (United States)

    Furtado, Guilherme H; Nicolau, David P


    The rapidly escalating prevalence of antimicrobial resistance is a global concern. This reduced susceptibility to currently available antimicrobial agents coupled with the progressive shortage of newly approved compounds is a worrisome situation. Major problems are encountered for a growing number of Gram-positive (i.e., Staphylococcus aureus, Streptococcus pneumoniae, Enterococcus spp.) and Gram-negative pathogens (i.e., Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella pneumoniae). We provide an overview of bacterial resistance focusing on the most common pathogens responsible for infection in both the community and healthcare settings. In addition, several strategies to curb antimicrobial resistance are also discussed. It is increasingly evident that without the introduction of novel antimicrobial agents, a return to the clinical outcomes associated with the pre-antibiotic era are inevitable.

  20. Exometabolomic Profiling of Bacterial Cultures

    DEFF Research Database (Denmark)

    Honoré, Anders Hans

    as the application into other food matrices. The scope of the thesis was to develop and apply a chromatography mass spectrometry based metabolomic footprint workflow for the investigation of the mechanisms behind the antifungal properties of a co‐culture, consisting of Lactobacillus paracasei (LAB A...... chromatography/mass spectrometry (GC/MS). Data from the untargeted LC/MS analysis was processed using feature selection and subsequent multivariate data analysis. The antifungal properties of the co‐culture were composed of several components when fermented in the model system. The major contribution came from......Selected bacterial cultures are used on an industrial scale for biopreservation of fresh fermented dairy products. The selected cultures provide efficient inhibition of yeasts and molds and serve as an alternative to the use of purified or synthetic antimicrobials. Despite the fact that numerous...

  1. Antibiotic resistance of bacterial biofilms

    DEFF Research Database (Denmark)

    Hoiby, N.; Bjarnsholt, T.; Givskov, M.


    A biofilm is a structured consortium of bacteria embedded in a self-produced polymer matrix consisting of polysaccharide, protein and DNA. Bacterial biofilms cause chronic infections because they show increased tolerance to antibiotics and disinfectant chemicals as well as resisting phagocytosis...... to antibiotics. Biofilm growth is associated with an increased level of mutations as well as with quorum-sensing-regulated mechanisms. Conventional resistance mechanisms such as chromosomal beta-lactamase, upregulated efflux pumps and mutations in antibiotic target molecules in bacteria also contribute...... to the survival of biofilms. Biofilms can be prevented by early aggressive antibiotic prophylaxis or therapy and they can be treated by chronic suppressive therapy. A promising strategy may be the use of enzymes that can dissolve the biofilm matrix (e.g. DNase and alginate lyase) as well as quorum...

  2. Instability of expanding bacterial droplets. (United States)

    Sokolov, Andrey; Rubio, Leonardo Dominguez; Brady, John F; Aranson, Igor S


    Suspensions of motile bacteria or synthetic microswimmers, termed active matter, manifest a remarkable propensity for self-organization, and formation of large-scale coherent structures. Most active matter research deals with almost homogeneous in space systems and little is known about the dynamics of strongly heterogeneous active matter. Here we report on experimental and theoretical studies on the expansion of highly concentrated bacterial droplets into an ambient bacteria-free fluid. The droplet is formed beneath a rapidly rotating solid macroscopic particle inserted in the suspension. We observe vigorous instability of the droplet reminiscent of a violent explosion. The phenomenon is explained in terms of continuum first-principle theory based on the swim pressure concept. Our findings provide insights into the dynamics of active matter with strong density gradients and significantly expand the scope of experimental and analytic tools for control and manipulation of active systems.

  3. Low Reynolds Number Bacterial Robots (United States)

    Giesbrecht, Grant; Ni, Katha; Vock, Isaac; Rodenborn, Bruce

    The dynamics of prokaryotic motility in a fluid is important in a wide range of fields. Our experiment models the locomotion of bacteria with a robotic swimmer made using a computer controlled DC motor that drives a helical flagellum formed from welding wire. Because of its small size, a bacterium swimming in water is like our robot swimming in corn syrup. We compensate for the size difference by placing the robot in highly viscous silicone oil. Previous research measured helical propulsion of a swimmer far from a boundary. However proximity to a boundary strongly affects bacterial swimming. We have designed a system to precisely control the distance from the flagellum to the tank wall, and have made some of the first macroscopic measurements of boundary effects on helical propulsion.

  4. Corticosteroids for acute bacterial meningitis. (United States)

    Brouwer, Matthijs C; McIntyre, Peter; Prasad, Kameshwar; van de Beek, Diederik


    In experimental studies, the outcome of bacterial meningitis has been related to the severity of inflammation in the subarachnoid space. Corticosteroids reduce this inflammatory response. To examine the effect of adjuvant corticosteroid therapy versus placebo on mortality, hearing loss and neurological sequelae in people of all ages with acute bacterial meningitis. We searched CENTRAL (2015, Issue 1), MEDLINE (1966 to January week 4, 2015), EMBASE (1974 to February 2015), Web of Science (2010 to February 2015), CINAHL (2010 to February 2015) and LILACS (2010 to February 2015). Randomised controlled trials (RCTs) of corticosteroids for acute bacterial meningitis. We scored RCTs for methodological quality. We collected outcomes and adverse effects. We performed subgroup analyses for children and adults, causative organisms, low-income versus high-income countries, time of steroid administration and study quality. We included 25 studies involving 4121 participants (2511 children and 1517 adults; 93 mixed population). Four studies were of high quality with no risk of bias, 14 of medium quality and seven of low quality, indicating a moderate risk of bias for the total analysis. Nine studies were performed in low-income countries and 16 in high-income countries.Corticosteroids were associated with a non-significant reduction in mortality (17.8% versus 19.9%; risk ratio (RR) 0.90, 95% confidence interval (CI) 0.80 to 1.01, P value = 0.07). A similar non-significant reduction in mortality was observed in adults receiving corticosteroids (RR 0.74, 95% CI 0.53 to 1.05, P value = 0.09). Corticosteroids were associated with lower rates of severe hearing loss (RR 0.67, 95% CI 0.51 to 0.88), any hearing loss (RR 0.74, 95% CI 0.63 to 0.87) and neurological sequelae (RR 0.83, 95% CI 0.69 to 1.00).Subgroup analyses for causative organisms showed that corticosteroids reduced mortality in Streptococcus pneumoniae (S. pneumoniae) meningitis (RR 0.84, 95% CI 0.72 to 0.98), but not in

  5. Autoproteolytic Activation of Bacterial Toxins

    Directory of Open Access Journals (Sweden)

    Aimee Shen


    Full Text Available Protease domains within toxins typically act as the primary effector domain within target cells. By contrast, the primary function of the cysteine protease domain (CPD in Multifunctional Autoprocessing RTX-like (MARTX and Clostridium sp. glucosylating toxin families is to proteolytically cleave the toxin and release its cognate effector domains. The CPD becomes activated upon binding to the eukaryotic-specific small molecule, inositol hexakisphosphate (InsP6, which is found abundantly in the eukaryotic cytosol. This property allows the CPD to spatially and temporally regulate toxin activation, making it a prime candidate for developing anti-toxin therapeutics. In this review, we summarize recent findings related to defining the regulation of toxin function by the CPD and the development of inhibitors to prevent CPD-mediated activation of bacterial toxins.

  6. Anaerobic bacterial meningitis in adults. (United States)

    Lee, Jun-Jun; Lien, Chia-Yi; Chien, Chun-Chih; Huang, Chi-Ren; Tsai, Nai-Wen; Chang, Chiung-Chih; Lu, Cheng-Hsien; Chang, Wen-Neng


    Anaerobic infection is a very uncommon condition in adult bacterial meningitis (ABM), and its clinical characteristics have yet to be clarified. We enrolled 540 patients with culture-proven bacterial meningitis during a study period of 30 years (1986-2015), of whom 13 (2.4%) had anaerobic infections. These 13 patients were eight men and five women, aged 22-77 years. Among them, 53.8% (7/13) had a postneurosurgical state as the preceding event, and 79.6% (10/13) had underlying medical conditions including diabetes mellitus, malignancy, liver cirrhosis, cerebral infarct and alcoholism. Nosocomial and mixed infections were found in 15.5% (2/13) and 46.1% (6/13) of the patients, respectively. A total of 14 anaerobic strains were isolated from cerebrospinal fluid specimens, including nine Gram-negative (G(-)) strains: Fusobacterium nucleatum (3), Prevotella species (3) and Bacteroides fragilis (3), and five Gram-positive (G(+)) strains: Propionibacterium acnes (3) and Peptostreptococcus micros (also known as Parvimonas micra) (2). All of the implicated G(+) anaerobic bacteria were susceptible to penicillin, and no multiple drug-resistant strains were found among the implicated G(-) anaerobic bacteria. Despite treatment, 30.8% (4/13) of the patients died. Of the nine survivors, 22.2% (2/9) had a full recovery, while the other 77.8% (7/9) had varying degrees of neurological deficits. Compared with the good outcome group (n = 6, modified Rankin scale (mRS) scores: 0-2), the poor outcome group (n = 7, mRS scores ≧3) had higher incidence of seizure. These results may offer a preliminary view of the clinical characteristics of anaerobic ABM. Copyright © 2018 Elsevier Ltd. All rights reserved.

  7. Pituitary apoplexy initially mistaken for bacterial meningitis


    Wong, Sui Hsien; Das, Kumar; Javadpour, Mohsen


    We presented a case of a 62-year-old man whose initial clinical picture was suggestive of bacterial meningitis, but instead had pituitary apoplexy. We highlighted how pituitary apoplexy can mimic bacterial meningitis, learning points on how clinical assessment can aid earlier diagnosis and the importance of considering this differential diagnosis, particularly with the associated morbidity and mortality if missed.

  8. neonatal bacterial meningitis in Cape Town children

    African Journals Online (AJOL)

    neonatal bacterial meningitis in Cape Town children. Bacterial meningitis is a major cause of childhood morbidity and mortality in South Africa. However, comprehensive regional or national epidemiological data, essential for rational public health interventions, are lacking. The purpose of this 1-year prospective study, from.

  9. Proteomic Analysis of Bacterial Expression Profiles Following ...

    African Journals Online (AJOL)

    Bacterial proteins were then extracted from the cell pellets and culture supernatants, using bacterial protein extraction reagent (Thermo Scientific) and ammonium sulfate precipitation. SDS-PAGE gel electrophoresis and protein sequence analysis. SDS-PAGE gel electrophoresis was performed using 12 % resolving gel [1.5 ...

  10. Use of Bacteriophages to control bacterial pathogens (United States)

    Lytic bacteriophages can provide a natural method and an effective alternative to antibiotics to reduce bacterial pathogens in animals, foods, and other environments. Bacteriophages (phages) are viruses which infect bacterial cells and eventually kill them through lysis, and represent the most abun...

  11. Joshua Lederberg's Legacy to Bacterial Genetics

    Indian Academy of Sciences (India)


    team which developed the artificial intelligence software program DENDRAL. ... to clear up all the uncertainties and lay the firm foundations of modern bacterial genetics. In order to understand and appreciate. As far as bacterial genetics is concerned, even the very existence ... Do they exist before exposure to the selective.

  12. Bacterial biofilms: prokaryotic adventures in multicellularity

    DEFF Research Database (Denmark)

    Webb, J.S.; Givskov, Michael Christian; Kjelleberg, S.


    The development of bacterial biofilms includes both the initial social behavior of undifferentiated cells, as well as cell death and differentiation in the mature biofilm, and displays several striking similarities with higher organisms. Recent advances in the field provide new insight...... into differentiation and cell death events in bacterial biofilm development and propose that biofilms have an unexpected level of multicellularity....

  13. Corrosion, haemocompatibility and bacterial adhesion behaviour of ...

    Indian Academy of Sciences (India)

    Bacterial adhesion, haemocompatibility and corrosion behaviour of TiZrN coating were examined in order to evaluate the coating's compatibility for ideal implant. Results revealed that TiZrN coatings exhibited less bacterial attachment against Staphylococcus aureus and Escherichia coli bacteria, negligible platelets ...

  14. Periarrest intestinal bacterial translocation and resuscitation outcome. (United States)

    Chalkias, Athanasios; Scheetz, Marc H; Gulati, Anil; Xanthos, Theodoros


    During the periarrest period, intestinal ischemia may result in barrier dysfunction and bacterial translocation, which has clear mechanistic links to inflammation and cascade stimulation, especially in patients who are treated with therapeutic hypothermia. Despite optimal management, periarrest bacterial translocation may worsen the outcome of cardiac arrest victims. Copyright © 2015 Elsevier Inc. All rights reserved.

  15. 7 CFR 58.135 - Bacterial estimate. (United States)


    ... Specifications for Dairy Plants Approved for USDA Inspection and Grading Service 1 Quality Specifications for Raw Milk § 58.135 Bacterial estimate. (a) Methods of Testing. Milk shall be tested for bacterial estimate... representative sample of each producer's milk at least once each month at irregular intervals. Samples shall be...

  16. Susceptibility of Some Bacterial Contaminants Recovered from ...

    African Journals Online (AJOL)

    Purpose: To investigate the susceptibility of bacterial contaminants recovered from cosmetics to preservatives and antibiotics. Methods: Nine bacterial isolates recovered from various brands of commercially available cosmetics marketed in Jordan were tested for their susceptibility pattern against two paraben esters and two ...

  17. Susceptibility of Some Bacterial Contaminants Recovered from ...

    African Journals Online (AJOL)

    Purpose: To investigate the susceptibility of bacterial contaminants recovered from cosmetics to preservatives and antibiotics. Methods: Nine bacterial isolates recovered from various brands of commercially available cosmetics marketed in Jordan were tested for their susceptibility pattern against two paraben esters and two.

  18. F{sup 19} relaxation in non-magnetic hexafluorides; Contribution a l'etude de la relaxation des fluors dans les hexafluorures non magnetiques

    Energy Technology Data Exchange (ETDEWEB)

    Rigny, P. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    The interesting properties of the fluorine magnetic resonance in the hexafluorides of molybdenum, tungsten and uranium, are very much due to large anisotropies of the chemical shift tensors. In the solid phases these anisotropies, the values of which are deduced from line shape studies, allow one to show that the molecules undergo hindered rotations about the metal atom. The temperature and frequency dependence of the fluorine longitudinal relaxation times shows that the relaxation is due to the molecular motion. The dynamical parameters of this motion are then deduced from the complete study of the fluorine relaxation in the rotating frame. In the liquid phases, the existence of anisotropies allows an estimation of the different contributions to the relaxation. In particular, the frequency and temperature dependence of the relaxation shows it to be dominated by the spin-rotation interaction. We have shown that the strength of this interaction can be deduced from the chemical shifts, and the angle through which the molecule rotates quasi-freely can be determined. In the hexafluorides, this angle is roughly one radian at 70 C, and with the help of this value, the friction coefficients which describe the intermolecular interactions are discussed. (author) [French] Les proprietes de la resonance magnetique des fluors dans les hexafluorures de molybdene, tungstene et uranium sont influencees par l'existence de deplacements chimiques tres anisotropes. Dans les phases solides, la valeur de cette anisotropie peut etre determinee par l'analyse des formes de raies et son existence permet de montrer que les molecules sont en rotation empechee autour de leur atome central. L'etude du temps de relaxation longitudinal en fonction de la temperature et de la frequence montre que la relaxation est due aux mouvements moleculaires, aux plus hautes temperatures. Les proprietes dynamiques du mouvement sont obtenues par l'etude complete de la relaxation spin

  19. C-reactive protein and bacterial meningitis

    DEFF Research Database (Denmark)

    Gerdes, Lars Ulrik; Jørgensen, P E; Nexø, E


    The aim of the study was to review published articles on the diagnostic accuracy of C-reactive protein (CRP) tests with cerebrospinal fluid and serum in diagnosing bacterial meningitis. The literature from 1980 and onwards was searched using the electronic databases of MEDLINE, and we used summary...... measured in serum, and 4 in which it had been measured in both cerebrospinal fluid and serum. The odds ratio for bacterial meningitis versus aseptic meningitis for a positive CRP test with cerebrospinal fluid was estimated at 241 (95% confidence interval [CI]: 59-980), and the central tendencies.......06-0.08, respectively, the post-test probability of not having bacterial meningitis given a negative test is very high (> or = 97%), in the range of a pre-test probability (prevalence of bacterial meningitis) from 10 to 30%, whereas the post-test probability of bacterial meningitis given a positive test is considerably...

  20. Calcification and Diagenesis of Bacterial Colonies

    Directory of Open Access Journals (Sweden)

    Ninon Robin


    Full Text Available Evidencing ancient interspecific associations in the fossil record may be challenging, particularly when bacterial organisms have most likely been degraded during diagenesis. Yet, documenting ancient interspecific associations may provide valuable insights into paleoenvironmental conditions and paleocommunities. Here, we report the multiscale characterization of contemporary and fossilized calcifying bacterial colonies found on contemporary shrimps from Mexico (La Paz Bay and on 160-Ma old fossilized decapods (shrimps from the Lagerstätte of La Voulte-sur-Rhône (France, respectively. We document the fine scale morphology, the inorganic composition and the organic signatures of both the contemporary and fossilized structures formed by these bacterial colonies using a combination of electron microscopies and synchrotron-based scanning transmission X-ray microscopy. In addition to discussing the mechanisms of carbonate precipitation by such bacterial colonies, the present study illustrates the degradation of bacterial remains occurring during diagenesis.

  1. Bacterial Biofilm Control by Perturbation of Bacterial Signaling Processes

    Directory of Open Access Journals (Sweden)

    Tim Holm Jakobsen


    Full Text Available The development of effective strategies to combat biofilm infections by means of either mechanical or chemical approaches could dramatically change today’s treatment procedures for the benefit of thousands of patients. Remarkably, considering the increased focus on biofilms in general, there has still not been invented and/or developed any simple, efficient and reliable methods with which to “chemically” eradicate biofilm infections. This underlines the resilience of infective agents present as biofilms and it further emphasizes the insufficiency of today’s approaches used to combat chronic infections. A potential method for biofilm dismantling is chemical interception of regulatory processes that are specifically involved in the biofilm mode of life. In particular, bacterial cell to cell signaling called “Quorum Sensing” together with intracellular signaling by bis-(3′-5′-cyclic-dimeric guanosine monophosphate (cyclic-di-GMP have gained a lot of attention over the last two decades. More recently, regulatory processes governed by two component regulatory systems and small non-coding RNAs have been increasingly investigated. Here, we review novel findings and potentials of using small molecules to target and modulate these regulatory processes in the bacterium Pseudomonas aeruginosa to decrease its pathogenic potential.

  2. Oral bacterial DNA findings in pericardial fluid

    Directory of Open Access Journals (Sweden)

    Anne-Mari Louhelainen


    Full Text Available Background: We recently reported that large amounts of oral bacterial DNA can be found in thrombus aspirates of myocardial infarction patients. Some case reports describe bacterial findings in pericardial fluid, mostly done with conventional culturing and a few with PCR; in purulent pericarditis, nevertheless, bacterial PCR has not been used as a diagnostic method before. Objective: To find out whether bacterial DNA can be measured in the pericardial fluid and if it correlates with pathologic–anatomic findings linked to cardiovascular diseases. Methods: Twenty-two pericardial aspirates were collected aseptically prior to forensic autopsy at Tampere University Hospital during 2009–2010. Of the autopsies, 10 (45.5% were free of coronary artery disease (CAD, 7 (31.8% had mild and 5 (22.7% had severe CAD. Bacterial DNA amounts were determined using real-time quantitative PCR with specific primers and probes for all bacterial strains associated with endodontic disease (Streptococcus mitis group, Streptococcus anginosus group, Staphylococcus aureus/Staphylococcus epidermidis, Prevotella intermedia, Parvimonas micra and periodontal disease (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola, Fusobacterium nucleatus, and Dialister pneumosintes. Results: Of 22 cases, 14 (63.6% were positive for endodontic and 8 (36.4% for periodontal-disease-associated bacteria. Only one case was positive for bacterial culturing. There was a statistically significant association between the relative amount of bacterial DNA in the pericardial fluid and the severity of CAD (p=0.035. Conclusions: Oral bacterial DNA was detectable in pericardial fluid and an association between the severity of CAD and the total amount of bacterial DNA in pericardial fluid was found, suggesting that this kind of measurement might be useful for clinical purposes.

  3. ADN bacteriano en pacientes con cirrosis y ascitis estéril: Papel como marcador de translocación bacteriana y herramienta pronóstica Bacterial DNA in patients with cirrhosis ans sterile ascites: Its role as a marker of bacterial translocation and prognosis tool

    Directory of Open Access Journals (Sweden)

    J. M. González-Navajas


    Full Text Available Durante la última década hemos presenciado un aumento de la cantidad de datos relativos a la presencia de translocación bacteriana en los modelos experimentales de cirrosis. Sin embargo, los estudios clínicos se han visto limitados por la falta de métodos no invasivos para estudiar dicho fenómeno. En los últimos años, las investigaciones realizadas en nuestro laboratorio se han centrado en la detección del ADN bacteriano en el suero y el líquido ascítico de los pacientes con cirrosis y ascitis estéril, y en las implicaciones clínicas que ello conlleva. Al principio, gracias a un método basado en la reacción en cadena de la polimerasa (PCR y el secuenciamiento automatizado de nucleótidos, pudimos detectar e identificar la presencia de fragmentos de ADN bacteriano en dichos pacientes con ascitis no neutrocítica y con cultivo negativo. Desde entonces hemos acumulado una serie de datos que indican que la presencia de ADN bacteriano podría desempeñar un papel importante no sólo como marcador de translocación bacteriana, sino también como factor pronóstico a corto plazo. Expondremos aquí el pasado, el presente y el futuro de esta línea de investigación.During the last decade, we have witnessed an increase in the amount of data related with the presence of bacterial translocation in experimental models of cirrhosis. However, clinical studies have been limited by the lack of non-invasive methods to study this phenomenon. Over the past years, the research developed in our laboratory has been focused on the detection of bacterial DNA in serum and ascitic fluid of patients with cirrhosis and sterile ascites, the clinical and immunological implications of such finding. Initially, by means of a polymerase chain reaction (PCR-based method and automated nucleotide sequencing, we were able to detect and identify the presence of fragments of bacterial DNA in the mentioned patients with culture-negative, non-neutrocytic ascites. Since

  4. Influence of age on radioinduced cognitive disorders: Experimental studies with cerebral irradiation of 30 Gy in 10 sessions and 12 days in the Wistar rat at 1.5, 4 and 18 months; Influence de l'age sur les troubles cognitifs radioinduits : etudes experimentales avec irradiation cerebrale de 30 Gy en 10 seances et 12 jours chez le rat Wistar de 1 1/2, 4 et 18 mois

    Energy Technology Data Exchange (ETDEWEB)

    Lamproglou, I. [Lab. de Chimie et Biophysique des Traceurs, Faculte de Medecine Xavier Bichat, Paris (France); Baillet, F.; Boisserie, G.; Mazeron, J.J. [Service de Radiotherapie, Hopital de la Pitie-Salpetriere, Paris (France); Delattre, J.Y. [Dept. of Neurology and Inst. National de la Sante et de la Recherche Medicale, INSERM, Hopital de la Pitie-Salpetriere, Paris (France)


    The objective of this study was to determine the influence of age on the learning and memory dysfunction induced by cranial radiation in the male Wistar rat. Ninety-six 45-day-old, 70 4-month-old, and 78 18-month-old male rats were divided in two equal groups: (i) irradiated and (ii) control. A course of whole-brain radiation therapy (30 Gy in 10 fractions over 12 days) was administered to the irradiated group, while the control group received sham irradiation. Sequential behavioral studies including one and two-way avoidance tests were undertaken before and after the 7 months following radiation. The results suggest that radiation induced progressive and irreversible memory dysfunction in elderly (18-month-old) rats, but this effect was partial or almost reversible in the 4-month-old and 45-day-old rats, respectively. In return, the learning dysfunction was age non-dependent despite the fact that is occurs more rapidly in the young (45 days, 4 months) rats. (author)

  5. Experimental study and modeling of CH{sub 4}/O{sub 2}/Ar and C{sub 2}H{sub 6}/O{sub 2}/Ar pre-mixing laminar flames; Etude experimentale et modelisation de flammes laminaires de premelange CH{sub 4}/O{sub 2}/Ar et C{sub 2}H{sub 6}/O{sub 2}/Ar

    Energy Technology Data Exchange (ETDEWEB)

    Crunelle, B.; Desgroux, P.; Pauwels, J.F. [Lille-1 Univ., 59 - Villeneuve-d`Ascq (France). Laboratoire de Cinetique et Chimie de la Combustion URA-CNRS


    New studies are always needed to better determine the physico-chemical processes involved in the combustion of natural gas. The understanding of the reaction mechanisms that lead to the formation of nitrogen oxides or volatile organic compounds requires to identify the inner mechanisms which take place during combustion and in particular the mechanisms of formation of intermediate products. The aim of this study is to analyze the thermal degradation of methane and ethane in low pressure pre-mixed stabilized laminar flames condition, because both of these compounds represent the major part of natural gas composition. The main chemical reaction ways identified in the studied flames and responsible for combustion have been identified after a comparison between experimental results and the computerized simulation performed using an a-priori postulated chemical mechanism. This study stresses on the transfer reaction schemes between the different C1, C2 and C3 oxidation ways which play an important role in the formation of intermediate hydrocarbons. (J.S.) 13 refs.

  6. Incineration of oxygenated volatile organic compounds. Experimental study and kinetic modeling of the oxidation of methyl ethyl ketone, ethyl acetate and butan-2-ol in methane flames; Incineration de composes organiques volatils oxygenes. Etude experimentale et modelisation cinetique de l'oxydation de la methyl ethyl cetone, de l'acetate d'ethyle et du butan-2-ol dans des flammes de methane

    Energy Technology Data Exchange (ETDEWEB)

    Decottignies, V.


    This work deals with the low pressure (0.05 atm) degradation of three volatile organic compounds (VOCs): methyl-ethyl-ketone, ethyl acetate and butan-2-ol, in premixed stoichiometric laminar methane flames seeded with 1 to 3% of each VOC. Molar fraction profiles of species have been obtained using microprobe sampling coupled with a gas chromatography and a mass spectroscopy analysis. Temperature profiles have been obtained using the covered thermocouple technique in the presence of the microprobe. The addition of a VOC in the initial reagents mixture leads to an increase of the quantity of intermediate hydrocarbon compounds and in particular of some soot precursor species. The degradation of VOCs leads to the formation of oxygenated intermediates like methanol, dimethyl-ether, acetaldehyde, propanal, acetone and vinyl acetate, the type of VOC having an effect on the quantities produced. The degradation of a VOC can lead to the formation of more toxic or polluting compounds (methyl vinyl ketone, acetic acid and acrolein) than the VOC itself. In the conditions of the study, the intermediate compounds are totally destructed inside the reactional area of the flame front and are no more present in the burnt gases. Sub-mechanisms of VOC oxidation have been developed using experimental observations and the most recent recommendations of the literature. These sub-mechanisms comprise 49 species involved in 241 elementary reactions. Their validation has been performed by comparing the experiment with the kinetic modeling on the molar fraction profiles of the detected species. Experimental data are well reproduced by the model for most species. The addition of a VOC inside the initial reagents mixture creates an important reactivity increase, in particular in the case of butan-2-ol seeded flames. The analysis of reactional ways has permitted to draw out the main reactions responsible for the degradation of the 3 VOCs and the ways of formation and consumption of the intermediate compounds specific of these VOCs. (J.S.)

  7. Experimental study and modeling of the propagation of an alkaline concentration wave coming from a cement matrix an passing through the argilite of the Meuse / Haute-Marne laboratory; Etude experimentale et modelisation de la propagation d'une onde de concentration alcaline issue d'une matrice cimentiere a travers l'argilite du site du laboratoire Meuse / Haute-Marne

    Energy Technology Data Exchange (ETDEWEB)

    Roussel, Th


    The propagation of an alkaline wave through a clay rock has been investigated- The wave is generated by a cementitious matrix through the Callovo-Oxfordian argillite of the Meuse Haute-Marne Laboratory (-480 m depth). The argillite itself is composed of quartz, micas, calcite and an interstratified l/S. In order to characterise the interactions between the alkaline fluid and the argillaceous medium, dynamic column experiments have been carried out. The originality of the investigation methodology consists in exploiting the data generated from the breakthrough curves as well as from the characterisation of the solids extracted from the columns. Two types of processes having totally different reaction times have hence been thoroughly studied: - Fast surface adsorption and condensation reactions: On the one hand cation adsorption reactions by site ionisation have been characterised. They are responsible of the buffering effect of the clays. On the other hand an original reaction of calcium compound condensation in the interlayer space of the swelling clays has been revealed. These processes have been modelled and their simulations with the IMPACT calculation code showed that the models elaborated were very satisfying. - Strongly kinetically limited dissolution/precipitation reactions: The main primary phases dissolved are quartz and interstratified l/S. The precipitation of secondary phases are mainly C(A)SH and zeolites. After the injection of an alkaline fluid for 6 months at 60 deg C, the argillite is strongly amorphized but only 20 to 30% of the quartz and the interstratified I/S are dissolved. Therefore, dissolution kinetics of the primary phases and the solubility products of the main secondary phases have been determined. (author)

  8. Experimental investigation of smectite hydration from the simulation of 001 X-ray diffraction lines. Implications for the characterization of mineralogical modifications of the 'argilite' from the Meuse - Haute Marne site as a result of a thermal perturbation; Etude experimentale de l'hydratation des smectites par simulation des raies OOl de diffraction des rayons X. Implications pour l'etude d'une perturbation thermique sur la mineralogie de l'argilite du site Meuse-Haute Marne

    Energy Technology Data Exchange (ETDEWEB)

    Ferrage, E


    The structural modifications affecting the reactive mineral constituents of the clay barriers (smectite) and possibly resulting from the thermal pulse related to nuclear waste storage are essentially limited to the amount and location of the layer charge deficit. These modifications likely impact the hydration properties of these minerals, and a specific methodology has thus been developed to describe, using simulation of X-ray diffraction profiles (001 reflections), these hydration properties and specifically the heterogeneity resulting from the inter-stratification of different layer types, each exhibiting a specific hydration state. The detailed study of the hydration properties of a low-charge montmorillonite (octahedral charge) has shown that the affinity of the interlayer cation for water rules the hydration state and the thickness of hydrated smectite layers. If the layer charge is increased, the transition between the different hydration states is shifted, following a water desorption isotherm, towards lower relative humidities. In addition, the hydration of studied beidellites (tetrahedral charge) was shown to be more heterogeneous than that of montmorillonites. The developed methodology also allowed describing the structural modifications resulting from a chemical perturbation (chlorinated anionic background, pH). Finally, the link between the thickness of elementary layers and the amount of interlayer water molecules has been evidenced. A new structure model has also been determined for these interlayer species allowing an improved description of their positional distribution in bi-hydrated interlayers. (author)

  9. Contribution to the study of the stability of water-cooled reactors; Contribution a l'etude de la stabilite des reacteurs refroidis par de l'eau

    Energy Technology Data Exchange (ETDEWEB)

    Coudert, C. [Commissariat a l' Energie Atomique, Cadarache (France). Centre d' Etudes Nucleaires


    This work is devoted to the study of the stability of reactors cooled by water subjected only to natural convection. It is made up of two parts, a theoretical study and experimental work, each of these parts being devoted to a consideration of linear and non-linear conditions: - calculation of the transfer function of the reactor using neutronic and hydrodynamic linear equations with the determination of the instability threshold; - demonstration of the existence of the limiting oscillation cycle in the case of a linear feedback using MALKIN'S method; - measurement and interpretation of the reactor's transfer functions and of the hydrodynamic transfer functions; and - analysis of the noise due to boiling. (author) [French] Dans ce travail on etudie la stabilite des piles refroidies par de l'eau circulant en convection naturelle. Cette etude se divise en deux parties: un travail theorique et un travail experimental, chacune de ces parties comportant une etude lineaire et une etude non-lineaire: - calcul de la fonction de transfert du reacteur a partir des equations lineaires de la neutronique et de l'hydrodynamique avec determination du seuil d'instabilite; - demonstration de l'existence du cycle limite des oscillations dans le cas d'une retroaction lineaire en utilisant la methode de MALKIN; - mesure et interpretation de la fonction de transfert du reacteur et des fonctions de transfert hydrodynamiques; et - analyse du bruit d'ebullition. (auteur)

  10. Bacterial Histidine Kinases as Novel Antibacterial Drug Targets

    NARCIS (Netherlands)

    Bem, A.E.; Velikova, N.R.; Pellicer, M.T.; Baarlen, van P.; Marina, A.; Wells, J.M.


    Bacterial histidine kinases (HKs) are promising targets for novel antibacterials. Bacterial HKs are part of bacterial two-component systems (TCSs), the main signal transduction pathways in bacteria, regulating various processes including virulence, secretion systems and antibiotic resistance. In

  11. Community-acquired bacterial meningitis in alcoholic patients

    NARCIS (Netherlands)

    Weisfelt, M.; de Gans, J.; van der Ende, A.; van de Beek, D.


    BACKGROUND: Alcoholism is associated with susceptibility to infectious disease, particularly bacterial pneumonia. In the present study we described characteristics in alcoholic patients with bacterial meningitis and delineate the differences with findings in non-alcoholic adults with bacterial

  12. The study of some thiazinic and indaminic dye syntheses induced by ionising radiation; Etude de quelques syntheses de colorants thianziniques et indaminiques amorcees par les rayonnements ionisants

    Energy Technology Data Exchange (ETDEWEB)

    Balestic, S. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    With a view to finding some radiochemical reactions applicable on an industrial scale for evaluating the radioactive waste from nuclear reactors, a systematic study was made of the radiochemical synthesis of thiazinic dyes such as methylene blue and Lauths' violet, on which the first tests were carried out in 1954. The first part of the study concerned the identification and the dosage, during radiolysis, of dyes by means of their absorption spectra after separation from the reaction medium by adsorption chromatography or ion-exchange; other radiolysis products such as ammonium chloride and hydrogen peroxide were also identified. During a later stage by systematically varying the physico-chemical parameters it was possible to determine the most favourable conditions for radio-synthesis; the maximum radiochemical yields obtained had the following values: G (Lauths' violet) 1,65; G (Methylene blue) = 1,75. Furthermore, the study of the influence of variously substituted aminated products on the radiochemical yield showed the possibility of synthesising Bindsehedlers green and Wursters blue by radiochemical methods. Finally the discovery of a fundamental intermediate product, Wursters red, together with the kinetic study of the chemical synthesis of methylene blue made it possible to determine the main stages of the reaction mechanism and to decide which of these stages could be attributed to ionising radiations in the case of the radiochemical synthesis. (author) [French] Dans le but de trouver des reactions radiochimiques susceptibles d'une application industrielle pour valoriser les dechets radioactifs provenant des reacteurs nucleaires, il a ete entrepris une etude systematique de la synthese radiochimique des colorants thiazimiques tels que le Bleu de Methylene et le Violet de Lauth dont les premiers essais ont ete effectues par Loiseleur en 1954. La premiere partie de l'etude a porte sur l'identification et le dosage des colorants formes

  13. Magnetic study of solid uranium-fluorine complexes; Contribution a l'etude magnetique de composes fluores solides de l'uranium

    Energy Technology Data Exchange (ETDEWEB)

    Dianoux, A.J. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    A study of the magnetic susceptibility of uranium V fluorine complexes and of the magnetic resonance of fluorine atoms in uranium VI fluorine complexes has made it possible to put forward a structural model for these compounds for which it is impossible, because of the lack of suitable single crystals for X-ray diffraction work, to deduce the exact position of the fluorine atoms. It is shown that it is difficult to interpret the paramagnetism of uranium fluorides, because the uranium ions are in low-symmetry sites. A theoretical study of the magnetism of the U{sup V} ion in complex fluorides of the type M{sub 3}UF{sub 8} (M = NH{sub 4}, Na, Rb, Cs) leads to an interpretation based on a trigonal deformation of the eight fluorine atom structure surrounding the uranium atom. By applying a Hamiltonian spin formalism and making a systematic use of group theory, it is possible to present the susceptibility calculations very concisely. Study of the resonance and of the relaxation of the fluorine atoms in powdered uranium VI complex fluorides suggests a structural model in the case of NaUF{sub 7}. It is shown that the shape of the magnetic resonance absorption lines is strongly affected by the presence of large anisotropic chemical shifts. In the model proposed here, six fluorine atoms are linked to the uranium, atom by strongly covalent bonds in a deformed UF{sub 6} octahedral structure; the seventh fluorine atom remains ionic. The occurrence of a rotational movement of the octahedron is confirmed by a study of the longitudinal relaxation of the fluorine atoms, the activation energy being 0.46 eV. (author) [French] L'etude de la susceptibilite magnetique de complexes fluores d'uranium V et la resonance magnetique des fluors dans des complexes fluores d'uranium VI permettent de proposer un modele structural pour ces composes, ou la diffraction des rayons X, en l'absence de monocristaux convenables, est incapable de preciser la position des atomes de

  14. Contribution to the study of the vertical molten zone process (1963); Contribution a l'etude du procede de la zone fondue verticale (1963)

    Energy Technology Data Exchange (ETDEWEB)

    Lenzin, M. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    Construction and use of several molten zone apparatuses operating either vertically or horizontally. Efficient purification of uranyl nitrate hexahydrate but less successful in the case of other hydrated double salts and of zirconyl chloride in the hydrochloric gel form. Demonstration and study of the dissymmetry in the direction of the transport of the impurity during, the purification by a vertical molten zone process. (author) [French] Construction et utilisation de plusieurs appareils de zone fondue travaillant soit en vertical, soit en horizontal. Purification efficace du nitrate d'uranyle hexahydrate mais peu significative dans le cas des autres couples de sels hydrates et du chlorure de zirconyle a l'etat de gel chlorhydrique. Mise en evidence et etude de la dissymetrie sur le sens de transport de l'impurete au cours de la purification par zone fondue verticale. (auteur)

  15. Radionuclide scintigraphy of bacterial nephritis

    Energy Technology Data Exchange (ETDEWEB)

    Conway, J.J.; Weiss, S.C.; Shkolnik, A.; Yogev, R.; Firlit, C.; Traisman, E.S.


    Pyelonephritis is a leading cause of renal failure and is expected to cost as much as three billion dollars in 1984. The diagnosis of urinary tract infection is usually not difficult. However, localization of the infection within the renal parenchyma as opposed to the collecting system is much more difficult. Flank pain, fever, bacteiuria and evidence of parenchymal involvement by intravenous urography may be absent or unrecognized particularly in the infant. Ultrasound and Nuclear Medicine are advocated as better methods to define parenchymal involvement. Such definition is important in the consideration of treatment since parenchymal involvement of the kidney carries a much more ominous potential outcome than infection restricted to within the collecting system. 38 children with a clinical diagnosis of urinary tract infection were studied. 26 of the patients demonstrated abnormal renal parenchymal findings with Gallium-67 Citrate or Tc-99m Glucoheptonate scintigraphy. Intravenous urography was notably ineffective with only 5 of the 20 interpreted as abnormal due to parenchymal disease or decreased function. 11 were entirely normal while only 5 demonstrated scars or hydronephrosis. Only 10 of 17 patients demonstrated intranvesicoureteral reflux on x-ray or nuclear cystography. Ultrasound depicted 6 of 20 patients as having parenchymal abnormalities. Seven were normal. Nonspecific findings such as dilitation of the renal pelvis or renal enlargement was noted in 11 of the 20 patients. Radionuclide Scintigraphy is the most efficacious modality to detect since acute bacterial nephritis.

  16. Gut bacterial microbiota and obesity. (United States)

    Million, M; Lagier, J-C; Yahav, D; Paul, M


    Although probiotics and antibiotics have been used for decades as growth promoters in animals, attention has only recently been drawn to the association between the gut microbiota composition, its manipulation, and obesity. Studies in mice have associated the phylum Firmicutes with obesity and the phylum Bacteroidetes with weight loss. Proposed mechanisms linking the microbiota to fat content and weight include differential effects of bacteria on the efficiency of energy extraction from the diet, and changes in host metabolism of absorbed calories. The independent effect of the microbiota on fat accumulation has been demonstrated in mice, where transplantation of microbiota from obese mice or mice fed western diets to lean or germ-free mice produced fat accumulation among recipients. The microbiota can be manipulated by prebiotics, probiotics, and antibiotics. Probiotics affect the microbiota directly by modulating its bacterial content, and indirectly through bacteriocins produced by the probiotic bacteria. Interestingly, certain probiotics are associated with weight gain both in animals and in humans. The effects are dependent on the probiotic strain, the host, and specific host characteristics, such as age and baseline nutritional status. Attention has recently been drawn to the association between antibiotic use and weight gain in children and adults. We herein review the studies describing the associations between the microbiota composition, its manipulation, and obesity. © 2013 The Authors Clinical Microbiology and Infection © 2013 European Society of Clinical Microbiology and Infectious Diseases.

  17. Biosynthesis of bacterial aromatic polyketides. (United States)

    Zhan, Jixun


    Aromatic polyketides represent important members of the family of polyketides, which have displayed a wide assortment of bioactive properties, such as antibacterial, antitumor, and antiviral activities. Bacterial aromatic polyketides are mainly synthesized by type II polyketide synthases (PKSs). Whereas malonyl-CoA is exclusively used as the extender unit, starter units can vary in different aromatic polyketide biosynthetic pathways, leading to a variety of polyketide backbones. Once the polyketide chains are elongated by the minimal PKSs to the full length, the immediate tailoring enzymes including ketoreductases, oxygenases and cyclases will work on the nascent chains to form aromatic structures, which will be further decorated by those late tailoring enzymes such as methyltransferases and glycosyltransferases. The mechanistic studies on the biosynthetic pathways of aromatic polyketides such as oxytetracycline and pradimicin A have been extensively carried out in recent years. Engineered biosynthesis of novel "unnatural" polyketides has been achieved in heterologous hosts such as Streptomyces coelicolor and Escherichia coli. This review covers the most recent advances in aromatic polyketide biosynthesis, which provide new enzymes or methods for building novel polyketide biosynthetic machinery.

  18. Collective decisions among bacterial viruses (United States)

    Joh, Richard; Mileyko, Yuriy; Voit, Eberhard; Weitz, Joshua


    For many temperate bacteriophages, the decision of whether to kill hosts or enter a latent state depends on the multiplicity of infection. In this talk, I present a quantitative model of gene regulatory dynamics to describe how phages make collective decisions within host cells. Unlike most previous studies, the copy number of viral genomes is treated as a variable. In the absence of feedback loops, viral mRNA transcription is expected to be proportional to the viral copy number. However, when there are nonlinear feedback loops in viral gene regulation, our model shows that gene expression patterns are sensitive to changes in viral copy number and there can be a domain of copy number where the system becomes bistable. Hence, the viral copy number is a key control parameter determining host cell fates. This suggests that bacterial viruses can respond adaptively to changes in population dynamics, and can make alternative decisions as a bet-hedging strategy. Finally, I present a stochastic version of viral gene regulation and discuss speed-accuracy trade-offs in the context of cell fate determination by viruses.

  19. Inflammatory Biomarkers During Bacterial Acute Rhinosinusitis. (United States)

    Autio, Timo J; Koskenkorva, Timo; Koivunen, Petri; Alho, Olli-Pekka


    Diagnosis of bacterial acute rhinosinusitis is difficult. Several attempts have been made to clarify the diagnostic criteria. Inflammatory biomarkers are easily obtainable variables that could shed light on both the pathophysiology and diagnosis of bacterial acute rhinosinusitis. The purpose of this review article is to assess literature concerning the course of inflammatory biomarkers during acute rhinosinusitis and the use of inflammatory biomarkers in diagnosing bacterial acute rhinosinusitis. We included C-reactive protein, erythrocyte sedimentation rate, white blood cell counts, procalcitonin, and nasal nitric oxide in this review and found that especially elevated C-reactive protein and erythrocyte sedimentation rate are related to a higher probability of a bacterial cause of acute rhinosinusitis. Still, normal levels of these two biomarkers are quite common as well, or the levels can be heightened even during viral respiratory infection without suspicion of bacterial involvement. Elevated levels of C-reactive protein or erythrocyte sedimentation rate support diagnosis of bacterial acute rhinosinusitis, but due to a lack of sensitivity, they should not be used to screen patients for bacterial acute rhinosinusitis.

  20. Bacterial carbon cycling in a subarctic fjord

    DEFF Research Database (Denmark)

    Middelboe, Mathias; Glud, Ronnie N.; Sejr, Mikael Kristian


    of viruses on bacterial mortality (4–36% of cell production) and carbon cycling. Heterotrophic bacterial consumption was closely coupled with autochthonous BDOC production, and the majority of the primary production was consumed by pelagic bacteria at all seasons. The relatively low measured BGE emphasized......In this seasonal study, we examined the environmental controls and quantitative importance of bacterial carbon consumption in the water column and the sediment in the subarctic Kobbefjord, Greenland. Depth-integrated bacterial production in the photic zone varied from 5.0 ± 2.7 mg C m−2 d−1...... in February to 42 ± 28 mg C m−2 d−1 in May and 34 ± 7 mg C m−2 d−1 in September, corresponding to a bacterial production to primary production ratio of 0.34 ± 0.14, 0.07 ± 0.04, and 0.08 ± 0.06, respectively. Based on measured bacterial growth efficiencies (BGEs) of 0.09–0.10, pelagic bacterial carbon...

  1. Zoonotic bacterial meningitis in human adults. (United States)

    van Samkar, Anusha; Brouwer, Matthijs C; van der Ende, Arie; van de Beek, Diederik


    To describe the epidemiology, etiology, clinical characteristics, treatment, outcome, and prevention of zoonotic bacterial meningitis in human adults. We identified 16 zoonotic bacteria causing meningitis in adults. Zoonotic bacterial meningitis is uncommon compared to bacterial meningitis caused by human pathogens, and the incidence has a strong regional distribution. Zoonotic bacterial meningitis is mainly associated with animal contact, consumption of animal products, and an immunocompromised state of the patient. In a high proportion of zoonotic bacterial meningitis cases, CSF analysis showed only a mildly elevated leukocyte count. The recommended antibiotic therapy differs per pathogen, and the overall mortality is low. Zoonotic bacterial meningitis is uncommon but is associated with specific complications. The suspicion should be raised in patients with bacterial meningitis who have recreational or professional contact with animals and in patients living in regions endemic for specific zoonotic pathogens. An immunocompromised state is associated with a worse prognosis. Identification of risk factors and underlying disease is necessary to improve treatment. © 2016 American Academy of Neurology.

  2. Study of point defects in pure iron by means of electrical resistivity; Etude au moyen de la resistivite electrique des defauts ponctuels dans le fer pur

    Energy Technology Data Exchange (ETDEWEB)

    Minier-Cassayre, C. [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires


    In the first part of this work, after having reviewed the production, observation and the annealing of point defects In metals, we resume the present state of research. In the second part, we explain the techniques we have employed to produce point defects at low temperatures: irradiation, quenching and cold-work; and go on to the study of their migration and annealing. The experimental results obtained for pure iron and for iron containing certain impurities are presented in the third part. In the fourth part we suggest a model which explains the different stages of annealing observed, and their properties. We then compare the energies of interaction between point defects with the values deduced from the theory of elasticity. (author) [French] Dans la premiere partie de cette etude, apres avoir passe en revue la production, l'observation et les modes de guerison des defauts ponctuels dans les metaux, nous exposons l'etat actuel des recherches. La seconde partie est consacree aux techniques que nous avons employees pour produire des defauts ponctuels a basse temperature: irradiations, trempe et ecrouissage puis a l'etude de leur migration et de leur guerison. Les resultats experimentaux obtenus dans le fer pur et le fer contenant certaines impuretes sont presentes dans le troisieme chapitre. Nous proposons ensuite un modele qui explique les differents stades de guerison observes et leurs nombreuses proprietes: nous comparons les energies d'interaction entre defauts ponctuels aux valeurs que l'on pourrait deduire d'un modele elastique. (auteur)

  3. Etude d'un systeme de recuperation et de stockage de chaleur pour un systeme eolien-diesel avec stockage d'air comprime (United States)

    Remillon, Thibaut

    La recherche presentee dans ce memoire porte sur les systemes hybrides eolien-diesel avec stockage d'air comprime (SHEDAC) adaptes a la production d'electricite en sites isoles, et plus precisement sur l'apport d'un systeme de recuperation et de stockage de chaleur (TES) au fonctionnement de ces systemes. Le principe de fonctionnement est le suivant. Quand la puissance eolienne est excedentaire par rapport aux besoins de la charge, le surplus d'energie est utilise pour comprimer de l'air. De ce processus resulte un rechauffement de l'air, on choisit d'extraire et de stocker la chaleur separement. Lors de la phase de restitution d'energie, l'air comprime utilise pour suralimenter le moteur thermique est rechauffe en utili sant la chaleur stockee precedemment. Tout au long de l'etude, nous avons seulement pris en compte la chaleur provenant du processus de compression de l'air, et neglige en tous points du systeme les echanges de chaleur avec le milieu exterieur. Pour quantifier l'apport du systeme de stockage de chaleur, nous avons tout d'abord mene une etude bibliographique sur ces systemes, pour identifier les meilleures solutions disponibles. Ensuite, on a modelise une de ces solutions sous Simulink pour etudier son fonctionnement de maniere plus precise. D'autre part, on a modelise un systeme de stockage de chaleur ideal pour quantifier l'apport maximal d'un TES sur les performances du SHEDAC. Nous avons conclu que les TES les plus adaptes aux systemes SHEDAC etaient les solutions actives directes a deux reservoirs, et que, pour beneficier au maximum du systeme de stockage de chaleur, il etait interessant de realiser la detente de l'air comprime stocke dans un moteur a air comprime, pour maximiser la recuperation d'energie mecanique.

  4. Contribution to the study of deformed heavy nuclei by means of nuclear reactions; Contribution a l'etude des noyaux lourds deformes au moyen de reactions nucleaires

    Energy Technology Data Exchange (ETDEWEB)

    Gastebois, J. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    The experimental results obtained in the study of the (d,p) reactions, at E{sub d} = 12 MeV, on the three even-even deformed nuclei {sup 170}Yb, {sup 172}Yb and {sup 174}Yb have been analysed in terms of DWBA calculations. The spectroscopic information relative to the odd final nuclei have been compared with the predictions of the collective model and of the Nilsson's model. The effect of various parameters used in the DWBA analysis (form factors, optical wave functions) has been carefully studied. The observed differences between the three final nuclei are qualitatively reproduced in the experimental study of resonances, seen in excitation functions of elastically and inelastically scattered protons on the same target nuclei, and corresponding to analogue states in the three nuclei {sup 171}Lu, {sup 173}Lu and {sup 175}Lu. (author) [French] Les resultats experimentaux de l'etude des reactions (d.p) a E{sub d} = 12 MeV, sur les noyaux deformes pairs-pairs {sup 170}Yb, {sup 172}Yb et {sup 174}Yb ont ete interpretes dans le cadre de l'approximation de Born des ondes deformees. Les informations spectroscopiques relatives aux noyaux impairs finals ont ete comparees aux predictions du modele collectif et du modele de Nilsson, apres avoir examine avec soin l'influence des differents parametres (facteurs de forme, fonctions d'onde 'optiques') utilises lors de l'analyse. Les differences observees entre les trois noyaux finals sont qualitativement reproduites par les resultats experimentaux de l'etude de resonances dans les fonctions d'excitation de diffusion elastique et inelastique de protons sur les memes noyaux-cibles, lors de la recherche d'etats analogues dans les noyaux {sup 171}Lu, {sup 173}Lu et {sup 175}Lu. (auteur)

  5. Tobacco use increases susceptibility to bacterial infection

    Directory of Open Access Journals (Sweden)

    Demuth Donald R


    Full Text Available Abstract Active smokers and those exposed to secondhand smoke are at increased risk of bacterial infection. Tobacco smoke exposure increases susceptibility to respiratory tract infections, including tuberculosis, pneumonia and Legionnaires disease; bacterial vaginosis and sexually transmitted diseases, such as chlamydia and gonorrhoea; Helicobacter pylori infection; periodontitis; meningitis; otitis media; and post-surgical and nosocomial infections. Tobacco smoke compromises the anti-bacterial function of leukocytes, including neutrophils, monocytes, T cells and B cells, providing a mechanistic explanation for increased infection risk. Further epidemiological, clinical and mechanistic research into this important area is warranted.

  6. Spontaneous Bacterial Peritonitis in Subclinical Hypothyroidism

    Directory of Open Access Journals (Sweden)

    Dalip Gupta


    Full Text Available Hypothyroidism is an uncommon cause of ascites. Here we describe a case of a 75 year-old female patient with spontaneous bacterial peritonitis and subclinical hypothyroidism that resolved with thyroid replacement and antibiotic therapy respectively. Ascitic fluid analysis revealed a gram-positive bacterium on gram staining. A review of the literature revealed just one other reported case of myxoedema ascites with concomitant spontaneous bacterial peritonitis and no case has till been reported of spontaneous bacterial peritonitis in subclinical hypothyroidism.

  7. Bacterial leaching of uranium ores - a review

    International Nuclear Information System (INIS)

    Lowson, R.T.


    The bacterial leaching of uranium ores involves the bacterially catalysed oxidation of associated pyrite to sulphuric acid and Fe 3+ by autotrophic bacteria and the leaching of the uranium by the resulting acidic, oxidising solution. Industrial application has been limited to Thiobacillus thiooxidans and Thiobacillus ferrooxidans at pH 2 to 3, and examples of these are described. The bacterial catalysis can be improved with nutrients or prevented with poisons. The kinetics of leaching are controlled by the bed depth, particle size, percolation rate, mineralogy and temperature. Current work is aimed at quantitatively defining the parameters controlling the kinetics and extending the method to alkaline conditions with other autotrophic bacteria. (author)

  8. Bacterial binding to extracellular proteins - in vitro adhesion

    DEFF Research Database (Denmark)

    Schou, C.; Fiehn, N.-E.


    Viridans streptococci, bacterial adherence, extracellular matrix proteins, surface receptors, endocarditis......Viridans streptococci, bacterial adherence, extracellular matrix proteins, surface receptors, endocarditis...

  9. Study and achievement of a digital-analog-divider; Etude et realisation d'un diviseur-analogique-numerique

    Energy Technology Data Exchange (ETDEWEB)

    Petin, A. [Commissariat a l' Energie Atomique, Cadarache (France). Centre d' Etudes Nucleaires


    theorie du systeme, les paragraphes suivants presentent la recherche d'un schema synoptique, l'etude des circuits et la realisation. (auteur)

  10. Bacterial evolution: Resistance is a numbers game


    Hall, J.P.J.; Harrison, E.


    Plasmids are well known for spreading antibiotic-resistance genes between bacterial strains. Recent experiments show that they can also act as catalysts for evolutionary innovation, promoting rapid evolution of novel antibiotic resistance.

  11. Plant growth-promoting bacterial endophytes. (United States)

    Santoyo, Gustavo; Moreno-Hagelsieb, Gabriel; Orozco-Mosqueda, Ma del Carmen; Glick, Bernard R


    Bacterial endophytes ubiquitously colonize the internal tissues of plants, being found in nearly every plant worldwide. Some endophytes are able to promote the growth of plants. For those strains the mechanisms of plant growth-promotion known to be employed by bacterial endophytes are similar to the mechanisms used by rhizospheric bacteria, e.g., the acquisition of resources needed for plant growth and modulation of plant growth and development. Similar to rhizospheric plant growth-promoting bacteria, endophytic plant growth-promoting bacteria can act to facilitate plant growth in agriculture, horticulture and silviculture as well as in strategies for environmental cleanup (i.e., phytoremediation). Genome comparisons between bacterial endophytes and the genomes of rhizospheric plant growth-promoting bacteria are starting to unveil potential genetic factors involved in an endophytic lifestyle, which should facilitate a better understanding of the functioning of bacterial endophytes. Copyright © 2015 Elsevier GmbH. All rights reserved.

  12. Bacterial Clearance and Endocarditis in American Opossums (United States)

    Musher, Daniel M.; Richie, Yvonne


    The American opossum is the only experimental animal that regularly develops bacterial endocarditis spontaneously. There was no relation between the ability of opossums to clear bacteria from the bloodstream and the subsequent development of endocarditis. PMID:4208530

  13. Proteomic Analysis of Bacterial Expression Profiles Following ...

    African Journals Online (AJOL)

    mass spectrometry (GC-MS) were performed to determine the phytochemicals in the active fraction. Results: Five differentially expressed bacterial proteins (four from Escherichia coli and one from Staphylococcus aureus), were identified via ...

  14. Heterotrophic bacterial populations in tropical sandy beaches

    Digital Repository Service at National Institute of Oceanography (India)

    Nair, S.; LokaBharathi, P.A.

    Distribution pattern of heterotrophic bacterial flora of three sandy beaches of the west coast of India was studied. The population in these beaches was microbiologically different. Population peaks of halotolerant and limnotolerant forms were...

  15. Bacterial flora of the sigmoid neovagina

    NARCIS (Netherlands)

    T.A. Toolenaar; I. Freundt (Ingrid); J.H. Wagenvoort; F.J. Huikeshoven (Frans); M. Vogel; J. Jeekel (Hans); A.C. Drogendijk


    textabstractThe bacterial microbiota of 15 sigmoid neovaginas, created in patients with congenital vaginal aplasia or male transsexualism, was studied. No specimen was sterile, and only normal inhabitants of the colon were cultured. The total counts of bacteria were

  16. Bacterial strategies of resistance to antimicrobial peptides. (United States)

    Joo, Hwang-Soo; Fu, Chih-Iung; Otto, Michael


    Antimicrobial peptides (AMPs) are a key component of the host's innate immune system, targeting invasive and colonizing bacteria. For successful survival and colonization of the host, bacteria have a series of mechanisms to interfere with AMP activity, and AMP resistance is intimately connected with the virulence potential of bacterial pathogens. In particular, because AMPs are considered as potential novel antimicrobial drugs, it is vital to understand bacterial AMP resistance mechanisms. This review gives a comparative overview of Gram-positive and Gram-negative bacterial strategies of resistance to various AMPs, such as repulsion or sequestration by bacterial surface structures, alteration of membrane charge or fluidity, degradation and removal by efflux pumps.This article is part of the themed issue 'Evolutionary ecology of arthropod antimicrobial peptides'. © 2016 The Author(s).

  17. Identification and Characterization of Novel Biocontrol Bacterial

    Directory of Open Access Journals (Sweden)

    Young Cheol Kim


    Full Text Available Because bacterial isolates from only a few genera have been developed commercially as biopesticides, discovery and characterization of novel bacterial strains will be a key to market expansion. Our previous screen using plant bioassays identified 24 novel biocontrol isolates representing 12 different genera. In this study, we characterized the 3 isolates showing the best biocontrol activities. The isolates were Pantoea dispersa WCU35, Proteus myxofaciens WCU244, and Exiguobacterium acetylicum WCU292 based on 16S rRNA sequence analysis. The isolates showed differential production of extracellular enzymes, antimicrobial activity against various fungal or bacterial plant pathogens, and induced systemic resistance activity against tomato gray mold disease caused by Botrytis cinerea. E. acetylicum WCU292 lacked strong in vitro antimicrobial activity against plant pathogens, but induced systemic resistance against tomato gray mold disease. These results confirm that the trait of biological control is found in a wide variety of bacterial genera

  18. Bacterial endophytes enhance competition by invasive plants. (United States)

    Rout, Marnie E; Chrzanowski, Thomas H; Westlie, Tara K; DeLuca, Thomas H; Callaway, Ragan M; Holben, William E


    Invasive plants can alter soil microbial communities and profoundly alter ecosystem processes. In the invasive grass Sorghum halepense, these disruptions are consequences of rhizome-associated bacterial endophytes. We describe the effects of N2-fixing bacterial strains from S. halepense (Rout and Chrzanowski, 2009) on plant growth and show that bacteria interact with the plant to alter soil nutrient cycles, enabling persistence of the invasive. • We assessed fluxes in soil nutrients for ∼4 yr across a site invaded by S. halepense. We assayed the N2-fixing bacteria in vitro for phosphate solubilization, iron chelation, and production of the plant-growth hormone indole-3-acetic acid (IAA). We assessed the plant's ability to recruit bacterial partners from substrates and vertically transmit endophytes to seeds and used an antibiotic approach to inhibit bacterial activity in planta and assess microbial contributions to plant growth. • We found persistent alterations to eight biogeochemical cycles (including nitrogen, phosphorus, and iron) in soils invaded by S. halepense. In this context, three bacterial isolates solubilized phosphate, and all produced iron siderophores and IAA in vitro. In growth chamber experiments, bacteria were transmitted vertically, and molecular analysis of bacterial community fingerprints from rhizomes indicated that endophytes are also horizontally recruited. Inhibiting bacterial activity with antibiotics resulted in significant declines in plant growth rate and biomass, with pronounced rhizome reductions. • This work suggests a major role of endophytes on growth and resource allocation of an invasive plant. Indeed, bacterial isolate physiology is correlated with invader effects on biogeochemical cycles of nitrogen, phosphate, and iron.

  19. Heme Synthesis and Acquisition in Bacterial Pathogens


    Choby, Jacob E.; Skaar, Eric P.


    Bacterial pathogens require the iron-containing cofactor heme to cause disease. Heme is essential to the function of hemoproteins, which are involved in energy generation by the electron transport chain, detoxification of host immune effectors, and other processes. During infection, bacterial pathogens must synthesize heme or acquire heme from the host; however, host heme is sequestered in high-affinity hemoproteins. Pathogens have evolved elaborate strategies to acquire heme from host source...


    Three bacterial strains capable of utilizing caprolactam (hexahydro azepin-2-one) as the only source of nitrogen, carbon and energy were isolated...from active silt of aerotanks used to purify waste water from Caprolactam production. They were identified as Pseudomonas dacunnae and Bacterium agile 1...and 2. The P. dacunnae strain is most active with respect to Caprolactam . Bacterial utilization of caprolactam has a marked adaptive character

  1. Volatiles in Inter-Specific Bacterial Interactions. (United States)

    Tyc, Olaf; Zweers, Hans; de Boer, Wietse; Garbeva, Paolina


    The importance of volatile organic compounds for functioning of microbes is receiving increased research attention. However, to date very little is known on how inter-specific bacterial interactions effect volatiles production as most studies have been focused on volatiles produced by monocultures of well-described bacterial genera. In this study we aimed to understand how inter-specific bacterial interactions affect the composition, production and activity of volatiles. Four phylogenetically different bacterial species namely: Chryseobacterium, Dyella, Janthinobacterium, and Tsukamurella were selected. Earlier results had shown that pairwise combinations of these bacteria induced antimicrobial activity in agar media whereas this was not the case for monocultures. In the current study, we examined if these observations were also reflected by the production of antimicrobial volatiles. Thus, the identity and antimicrobial activity of volatiles produced by the bacteria were determined in monoculture as well in pairwise combinations. Antimicrobial activity of the volatiles was assessed against fungal, oomycetal, and bacterial model organisms. Our results revealed that inter-specific bacterial interactions affected volatiles blend composition. Fungi and oomycetes showed high sensitivity to bacterial volatiles whereas the effect of volatiles on bacteria varied between no effects, growth inhibition to growth promotion depending on the volatile blend composition. In total 35 volatile compounds were detected most of which were sulfur-containing compounds. Two commonly produced sulfur-containing volatile compounds (dimethyl disulfide and dimethyl trisulfide) were tested for their effect on three target bacteria. Here, we display the importance of inter-specific interactions on bacterial volatiles production and their antimicrobial activities.

  2. Volatiles in inter-specific bacterial interactions

    Directory of Open Access Journals (Sweden)

    Olaf eTyc


    Full Text Available The importance of volatile organic compounds for functioning of microbes is receiving increased research attention. However, to date very little is known on how inter-specific bacterial interactions effect volatiles production as most studies have been focused on volatiles produced by monocultures of well described bacterial genera. In this study we aimed to understand how inter-specific bacterial interactions affect the composition, production and activity of volatiles. Four phylogenetically different bacterial species namely: Chryseobacterium, Dyella, Janthinobacterium and Tsukamurella were selected. Earlier results had shown that pairwise combinations of these bacteria induced antimicrobial activity in agar media whereas this was not the case for monocultures. In the current study, we examined if these observations were also reflected by the production of antimicrobial volatiles. Thus, the identity and antimicrobial activity of volatiles produced by the bacteria were determined in monoculture as well in pairwise combinations. Antimicrobial activity of the volatiles was assessed against fungal, oomycetal and bacterial model organisms. Our results revealed that inter-specific bacterial interactions affected volatiles blend composition. Fungi and oomycetes showed high sensitivity to bacterial volatiles whereas the effect of volatiles on bacteria varied between no effects, growth inhibition to growth promotion depending on the volatile blend composition. In total 35 volatile compounds were detected most of which were sulfur-containing compounds. Two commonly produced sulfur-containing volatile compounds (dimethyl disulfide and dimethyl trisulfide were tested for their effect on three target bacteria. Here we display the importance of inter-specific interactions on bacterial volatiles production and their antimicrobial activities.

  3. Endocarditis in adults with bacterial meningitis. (United States)

    Lucas, Marjolein J; Brouwer, Matthijs C; van der Ende, Arie; van de Beek, Diederik


    Endocarditis may precede or complicate bacterial meningitis, but the incidence and impact of endocarditis in bacterial meningitis are unknown. We assessed the incidence and clinical characteristics of patients with meningitis and endocarditis from a nationwide cohort study of adults with community-acquired bacterial meningitis in the Netherlands from 2006 to 2012. Endocarditis was identified in 24 of 1025 episodes (2%) of bacterial meningitis. Cultures yielded Streptococcus pneumoniae in 13 patients, Staphylococcus aureus in 8 patients, and Streptococcus agalactiae, Streptococcus pyogenes, and Streptococcus salivarius in 1 patient each. Clues leading to the diagnosis of endocarditis were cardiac murmurs, persistent or recurrent fever, a history of heart valve disease, and S aureus as the causative pathogen of bacterial meningitis. Treatment consisted of prolonged antibiotic therapy in all patients and surgical valve replacement in 10 patients (42%). Two patients were treated with oral anticoagulants, and both developed life-threatening intracerebral hemorrhage. Systemic (70%) and neurological (54%) complications occurred frequently, leading to a high proportion of patients with unfavorable outcome (63%). Seven of 24 patients (29%) with meningitis and endocarditis died. Endocarditis is an uncommon coexisting condition in bacterial meningitis but is associated with a high rate of unfavorable outcome.

  4. The epidemiology of bacterial meningitis in Kosovo. (United States)

    Namani, Sadie A; Koci, Remzie A; Qehaja-Buçaj, Emine; Ajazaj-Berisha, Lindita; Mehmeti, Murat


    The purpose of this study was to present the epidemiologic features of bacterial meningitis in the developing country of Kosovo. Data were collected from active surveillance of bacterial meningitis cases treated at the University Clinical Center of Kosovo in the years 2000 (first post-war year) and 2010. Meningitis cases in 2000 compared with 2010 showed a 35.5% decline in incidence (from 4.8 to 3.1 cases per 100,000 population) and a decrease in the case fatality rate from 10% to 5%. In children, there was a lower mortality rate (5% versus 2%) and a lower incidence of neurological complications (13% versus 16%) as compared to adults (32% versus 10% and 16% versus 35%, respectively). Neisseria meningitidis was the most common pathogen of bacterial meningitis in both study periods. Bacterial meningitis was most prevalent in the pediatric population, and showed an increase in the median age, from three years in 2000 to seven years in 2010. A steady number of bacterial meningitis cases in adults throughout last decade (around 20 cases per year) was recorded. During the last decade, gradual changes have been observed in the epidemiology of bacterial meningitis that are unrelated to the introduction of new vaccines, but are partly due to the improvement of living conditions.

  5. The intrinsic resistome of bacterial pathogens

    Directory of Open Access Journals (Sweden)

    Jorge Andrés Olivares Pacheco


    Full Text Available Intrinsically resistant bacteria have emerged as a relevant health problem in the last years. Those bacterial species, several of them with an environmental origin, present naturally a low-level susceptibility to several drugs. It has been proposed that intrinsic resistance is mainly the consequence of the impermeability of cellular envelopes, the activity of multidrug efflux pumps or the lack of appropriate targets for a given family of drugs. However, recently published articles indicate that the characteristic phenotype of susceptibility to antibiotics of a given bacterial species depends on the concerted activity of several elements, what has been named as intrinsic resistome. These determinants comprise not just classical resistance genes. Other elements, several of them involved in basic bacterial metabolic processes, are of relevance for the intrinsic resistance of bacterial pathogens. In the present review we analyse recent publications on the intrinsic resistomes of Escherichia coli and Pseudomonas aeruginosa. We present as well information on the role that global regulators of bacterial metabolism, as Crc from P. aeruginosa, may have on modulating bacterial susceptibility to antibiotics. Finally, we discuss the possibility of searching inhibitors of the intrinsic resistome in the aim of improving the activity of drugs currently in use for clinical practice.

  6. Bacterial RNAs activate innate immunity in Arabidopsis. (United States)

    Lee, Boyoung; Park, Yong-Soon; Lee, Soohyun; Song, Geun Cheol; Ryu, Choong-Min


    The common molecular patterns of microbes play a critical role in the regulation of plant innate immunity. However, little is known about the role of nucleic acids in this process in plants. We pre-infiltrated Arabidopsis leaves with total RNAs from Pseudomonas syringae pv. tomato DC3000 (Pto DC3000) and subsequently inoculated these plants with the same bacterial cells. Total Pto DC3000 RNAs pre-infiltrated into Arabidopsis leaves elicited plant immune responses against Pto DC3000. However, sheared RNAs and RNase A application failed to induce immunity, suggesting that intact bacterial RNAs function in plant innate immunity. This notion was supported by the positive regulation of superoxide anion levels, callose deposition, two mitogen-activated protein kinases and defense-related genes observed in bacterial RNA-pre-treated leaves. Intriguingly, the Pto DC3000 population was not compromised in known pattern recognition receptor mutants for chitin, flagellin and elongation factor-Tu (EF-Tu). Plant defense-related mutant analyses further revealed that bacterial RNA-elicited innate immunity was normally required for salicylic and jasmonic acid signaling. Notably, among total RNAs, the abundant bacterial RNA species 16S and 23S ribosomal RNAs were the major determinants of this response. Our findings provide evidence that bacterial RNA serves as a microbe-associated molecular pattern in plants. © 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.

  7. Role of quorum sensing in bacterial infections (United States)

    Castillo-Juárez, Israel; Maeda, Toshinari; Mandujano-Tinoco, Edna Ayerim; Tomás, María; Pérez-Eretza, Berenice; García-Contreras, Silvia Julieta; Wood, Thomas K; García-Contreras, Rodolfo


    Quorum sensing (QS) is cell communication that is widely used by bacterial pathogens to coordinate the expression of several collective traits, including the production of multiple virulence factors, biofilm formation, and swarming motility once a population threshold is reached. Several lines of evidence indicate that QS enhances virulence of bacterial pathogens in animal models as well as in human infections; however, its relative importance for bacterial pathogenesis is still incomplete. In this review, we discuss the present evidence from in vitro and in vivo experiments in animal models, as well as from clinical studies, that link QS systems with human infections. We focus on two major QS bacterial models, the opportunistic Gram negative bacteria Pseudomonas aeruginosa and the Gram positive Staphylococcus aureus, which are also two of the main agents responsible of nosocomial and wound infections. In addition, QS communication systems in other bacterial, eukaryotic pathogens, and even immune and cancer cells are also reviewed, and finally, the new approaches proposed to combat bacterial infections by the attenuation of their QS communication systems and virulence are also discussed. PMID:26244150

  8. Exploring bacterial infections: theoretical and experimental studies of the bacterial population dynamics and antibiotic treatment (United States)

    Shao, Xinxian

    Bacterial infections are very common in human society. Thus extensive research has been conducted to reveal the molecular mechanisms of the pathogenesis and to evaluate the antibiotics' efficacy against bacteria. Little is known, however, about the population dynamics of bacterial populations and their interactions with the host's immune system. In this dissertation, a stochatic model is developed featuring stochastic phenotypic switching of bacterial individuals to explain the single-variant bottleneck discovered in multi strain bacterial infections. I explored early events in a bacterial infection establishment using classical experiments of Moxon and Murphy on neonatal rats. I showed that the minimal model and its simple variants do not work. I proposed modifications to the model that could explain the data quantitatively. The bacterial infections are also commonly established in physical structures, as biofilms or 3-d colonies. In contrast, most research on antibiotic treatment of bacterial infections has been conducted in well-mixed liquid cultures. I explored the efficacy of antibiotics to treat such bacterial colonies, a broadly applicable method is designed and evaluated where discrete bacterial colonies on 2-d surfaces were exposed to antibiotics. I discuss possible explanations and hypotheses for the experimental results. To verify these hypotheses, we investigated the dynamics of bacterial population as 3-d colonies. We showed that a minimal mathematical model of bacterial colony growth in 3-d was able to account for the experimentally observed presence of a diffusion-limited regime. The model further revealed highly loose packing of the cells in 3-d colonies and smaller cell sizes in colonies than plancktonic cells in corresponding liquid culture. Further experimental tests of the model predictions have revealed that the ratio of the cell size in liquid culture to that in colony cultures was consistent with the model prediction, that the dead cells

  9. Photoinactivation of major bacterial pathogens in aquaculture

    Directory of Open Access Journals (Sweden)

    Heyong Jin Roh


    Full Text Available Abstract Background Significant increases in the bacterial resistance to various antibiotics have been found in fish farms. Non-antibiotic therapies for infectious diseases in aquaculture are needed. In recent years, light-emitting diode technology has been applied to the inactivation of pathogens, especially those affecting humans. The purpose of this study was to assess the effect of blue light (wavelengths 405 and 465 nm on seven major bacterial pathogens that affect fish and shellfish important in aquaculture. Results We successfully demonstrate inactivation activity of a 405/465-nm LED on selected bacterial pathogens. Although some bacteria were not fully inactivated by the 465-nm light, the 405-nm light had a bactericidal effect against all seven pathogens, indicating that blue light can be effective without the addition of a photosensitizer. Photobacterium damselae, Vibrio anguillarum, and Edwardsiella tarda were the most susceptible to the 405-nm light (36.1, 41.2, and 68.4 J cm−2, respectively, produced one log reduction in the bacterial populations, whereas Streptococcus parauberis was the least susceptible (153.8 J cm−2 per one log reduction. In general, optical density (OD values indicated that higher bacterial densities were associated with lower inactivating efficacy, with the exception of P. damselae and Vibrio harveyi. In conclusion, growth of the bacterial fish and shellfish pathogens evaluated in this study was inactivated by exposure to either the 405- or 465-nm light. In addition, inactivation was dependent on exposure time. Conclusions This study presents that blue LED has potentially alternative therapy for treating fish and shellfish bacterial pathogens. It has great advantages in aspect of eco-friendly treating methods differed from antimicrobial methods.

  10. Antibiotics promote aggregation within aquatic bacterial communities

    Directory of Open Access Journals (Sweden)

    Gianluca eCorno


    Full Text Available The release of antibiotics (AB into the environment poses several threats for human health due to potential development of ABresistant natural bacteria. Even though the use of low-dose antibiotics has been promoted in health care and farming, significant amounts of AB are observed in aquatic environments. Knowledge on the impact of AB on natural bacterial communities is missing both in terms of spread and evolution of resistance mechanisms, and of modifications of community composition and productivity. New approaches are required to study the response of microbial communities rather than individual resistance genes. In this study a chemostat-based experiment with 4 coexisting bacterial strains has been performed to mimicking the response of a freshwater bacterial community to the presence of antibiotics in low and high doses. Bacterial abundance rapidly decreased by 75% in the presence of AB, independently of their concentration, and remained constant until the end of the experiment. The bacterial community was mainly dominated by Aeromonas hydrophila and Brevundimonas intermedia while the other two strains, Micrococcus luteus and Rhodococcus sp. never exceed 10%. Interestingly, the bacterial strains, which were isolated at the end of the experiment, were not AB-resistant, while reassembled communities composed of the 4 strains, isolated from treatments under AB stress, significantly raised their performance (growth rate, abundance in the presence of AB compared to the communities reassembled with strains isolated from the treatment without AB. By investigating the phenotypic adaptations of the communities subjected to the different treatments, we found that the presence of AB significantly increased co-aggregation by 5-6 fold.These results represent the first observation of co-aggregation as a successful strategy of AB resistance based on phenotype in aquatic bacterial communities, and can represent a fundamental step in the understanding of

  11. Cerebrospinal Fluid Cathelicidin Correlates With the Bacterial Load and Outcomes in Childhood Bacterial Meningitis. (United States)

    Savonius, Okko; Helve, Otto; Roine, Irmeli; Andersson, Sture; Saukkoriipi, Annika; González Mata, Antonio; Peltola, Heikki; Pelkonen, Tuula


    Large cerebrospinal fluid (CSF) bacterial load in bacterial meningitis (BM) relates to poor outcome. However, the antimicrobial peptide cathelicidin seems important to host defense. We studied how cathelicidin concentrations and bacterial load in CSF relate in childhood BM and to what extent they may predict the disease outcome. The patient data originated from a large prospective clinical trial in Latin America in 1996-2003 in which the CSF samples were collected on admission (CSF1) and 12-24 hours later (CSF2). The cathelicidin concentrations were measured by enzyme-linked immunosorbent assay and the CSF bacterial load by real-time polymerase chain reaction. This analysis comprised 76 children with meningitis caused by Haemophilus influenzae type b (n = 44), Streptococcus pneumoniae (n = 28) or Neisseria meningitidis (n = 4). The cathelicidin concentration correlated with the bacterial genome count in both samples (CSF1: ρ = 0.531, P < 0.001; CSF2: ρ = 0.553, P < 0.001). A high CSF1 ratio of cathelicidin to the bacterial genome count was associated with fewer audiologic sequelae (odds ratio: 0.11, 95% confidence interval: 0.02-0.61, P = 0.01) and more favorable neurologic outcomes (odds ratio: 3.95, 95% confidence interval: 1.22-12.8, P = 0.02), but not with better survival. In conclusion, CSF cathelicidin and the bacterial load were closely related in childhood BM. A high initial cathelicidin-to-bacterial genome count ratio predicted better outcomes in survivors.

  12. Bacterial genomes: habitat specificity and uncharted organisms. (United States)

    Dini-Andreote, Francisco; Andreote, Fernando Dini; Araújo, Welington Luiz; Trevors, Jack T; van Elsas, Jan Dirk


    The capability and speed in generating genomic data have increased profoundly since the release of the draft human genome in 2000. Additionally, sequencing costs have continued to plummet as the next generation of highly efficient sequencing technologies (next-generation sequencing) became available and commercial facilities promote market competition. However, new challenges have emerged as researchers attempt to efficiently process the massive amounts of sequence data being generated. First, the described genome sequences are unequally distributed among the branches of bacterial life and, second, bacterial pan-genomes are often not considered when setting aims for sequencing projects. Here, we propose that scientists should be concerned with attaining an improved equal representation of most of the bacterial tree of life organisms, at the genomic level. Moreover, they should take into account the natural variation that is often observed within bacterial species and the role of the often changing surrounding environment and natural selection pressures, which is central to bacterial speciation and genome evolution. Not only will such efforts contribute to our overall understanding of the microbial diversity extant in ecosystems as well as the structuring of the extant genomes, but they will also facilitate the development of better methods for (meta)genome annotation.

  13. Epidemiology of community-acquired bacterial meningitis. (United States)

    Brouwer, Matthijs C; van de Beek, Diederik


    The epidemiology of bacterial meningitis has been dynamic in the past 30 years following introduction of conjugated vaccines against Haemophilus influenzae type B, Streptococcus pneumoniae and Neisseria meningitidis. The purpose of this review is to describe recent developments in bacterial meningitis epidemiology. The incidence of bacterial meningitis in Western countries (Finland, Netherlands, and the United States) gradually declined by 3-4% per year to 0.7-0.9 per 100 000 per year in the past 10-20 years. In African countries (Burkina Faso and Malawi), incidence rates are still substantially higher at 10-40 per 100 000 persons per year. Introduction of pneumococcal conjugate vaccines have not consistently decreased overall pneumococcal meningitis incidence because of serotype replacement. Following the introduction of serogroup A and C meningococcal vaccines, the incidence of meningococcal meningitis because of these serogroups strongly decreased. Novel outbreaks in the African meningitis belt by serogroup C and increased incidence of serogroup W in the United Kingdom and the Netherlands were observed recently. Bacterial meningitis remains an important infectious disease, despite a gradual decline in incidence after large-scale vaccination campaigns. Further development of vaccines with broader coverage is important, as is continuous surveillance of bacterial meningitis cases.

  14. Immunity to bacterial infection in the chicken. (United States)

    Wigley, Paul


    Bacterial infections remain important to the poultry industry both in terms of animal and public health, the latter due to the importance of poultry as a source of foodborne bacterial zoonoses such as Salmonella and Campylobacter. As such, much focus of research to the immune response to bacterial infection has been to Salmonella. In this review we will focus on how research on avian salmonellosis has developed our understanding of immunity to bacteria in the chicken from understanding the role of TLRs in recognition of bacterial pathogens, through the role of heterophils, macrophages and γδ lymphocytes in innate immunity and activation of adaptive responses to the role of cellular and humoral immunity in immune clearance and protection. What is known of the immune response to other bacterial infections and in particular infections that have emerged recently as major problems in poultry production including Campylobacter jejuni, Avian Pathogenic Escherichia coli, Ornithobacterium rhinotracheale and Clostridium perfringens are discussed. Copyright © 2013 Elsevier Ltd. All rights reserved.

  15. Etude exploratoire des conceptions de la circulation sanguine aupres d'eleves de l'ordre collegial (United States)

    Robitaille, Jean-Marc

    Il existe peu d'etudes sur les conceptions touchant les domaines de la biologie, notamment sur les conceptions de la circulation sanguine Nous avons observe egalement l'absence de recherche menee aupres d'eleves de l'ordre collegial sur cette question. Nous avons voulu combler une lacune en menant une recherche sur les conceptions de la circulation sanguine aupres d'eleves de l'ordre collegial. Pour mener cette recherche nous nous sommes inspires d'une methode developpee par Treagust (1988). Le premier niveau de formulation didactique etablit l'architecture du systeme et la fonction nutritive de la circulation. Le second niveau de formulation didactique decrit et relie les parametres de la dynamique de la circulation et leur relation: Pression, Debit et Resistance. Le troisieme niveau de formulation didactique s'interesse au controle de la circulation du sang dans un contexte d'homeostasie qui implique la regulation de la pression arterielle. Nous avons construit un questionnaire en nous guidant sur les niveaux de formulation didactique et l'analyse des entrevues menees aupres de dix-huit eleves, representatifs de la population cible. Ce questionnaire fut administre a un echantillon de 2300 eleves disperses dans six colleges de la region de Montreal. Notre echantillon comprend des eleves inscrits a des programmes de l'ordre collegial en Sciences de la nature et en Techniques de la sante et qui n'ont pas suivi le cours sur la circulation sanguine. Notre analyse des reponses des eleves de notre echantillon aux questions sur le premier niveau de formulation didactique revele que la majorite des eleves considerent que le systeme circulatoire relie les organes les uns aux autres dans un circuit en serie. Notre analyse revele egalement que la majorite des eleves estiment que les nutriments sont extraits du sang par les organes selon un processus de selection base sur les besoins determines par la fonction de l'organe. Ces besoins sont differents selon les organes qui ne

  16. Investigation of the glide modes of single crystals of beryllium; Etude des modes de glissement de monocristaux de beryllium

    Energy Technology Data Exchange (ETDEWEB)

    Regnier, P. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires


    'observation des lignes de glissement et des configurations de dislocations. L'etude du glissement prismatique a ete developpee beaucoup plus largement: l'influence de la temperature de la vitesse de deformation, de l'orientation initiale du plan de base et des plans prismatiques ont ete releves sur des echantillons d'orientation soignee, deformes en tension. On a egalement effectue quelques essais de microdeformation et observe de facon detaillee les lignes de glissement et les configurations de dislocations. Le comportement tres particulier observe n'est pas du aux impuretes, mais au glissement devie. On propose un mecanisme de blocage des dislocations permettant de l'expliquer. Comme ce mecanisme suppose que les dislocations a-bar peuvent aussi se dissocier sur les plans prismatiques [101-bar0] on examine cette possibilite et l'on propose plusieurs dissociations eventuelles dont la plus probable correspond a la transformation de phase hexagonale compacte {yields} cubique centree. En generalisant cette hypothese, on montre alors comment on peut rendre compte de la facilite relative des divers modes de glissement dans tous les metaux hexagonaux. (auteur)

  17. Endolymphatic sac involvement in bacterial meningitis

    DEFF Research Database (Denmark)

    Møller, Martin Nue; Brandt, Christian; Østergaard, Christian


    The commonest sequelae of bacterial meningitis are related to the inner ear. Little is known about the inner ear immune defense. Evidence suggests that the endolymphatic sac provides some protection against infection. A potential involvement of the endolymphatic sac in bacterial meningitis...... is largely unaccounted for, and thus the object of the present study. A well-established adult rat model of Streptococcus pneumoniae meningitis was employed. Thirty adult rats were inoculated intrathecally with Streptococcus pneumoniae and received no additional treatment. Six rats were sham...... days. Bacteria invaded the inner ear through the cochlear aquaduct. On days 5-6, the bacteria invaded the endolymphatic sac through the endolymphatic duct subsequent to invasion of the vestibular endolymphatic compartment. No evidence of direct bacterial invasion of the sac through the meninges...

  18. Counterimmunoelectrophoresis in the diagnosis of bacterial meningitis

    DEFF Research Database (Denmark)

    Colding, H; Lind, I


    The aim of the present study was to investigate whether counterimmunoelectrophoresis (CIE) would facilitate the rapid, etiological diagnosis of bacterial meningitis when used in parallel with other routine methods in a medical bacteriological laboratory. Of 3,674 consecutive specimens of cerebros......The aim of the present study was to investigate whether counterimmunoelectrophoresis (CIE) would facilitate the rapid, etiological diagnosis of bacterial meningitis when used in parallel with other routine methods in a medical bacteriological laboratory. Of 3,674 consecutive specimens....../139) of the culture-negative specimens. CSF specimens from 21 patients with bacterial meningitis caused by other species were all negative in CIE, except four, three of which contained Escherichia coli antigen reacting with antiserum to N. meningitidis group B and one E. coli antigen reacting with antiserum to H...

  19. Evaluation of silicon oil on bacterial growth. (United States)

    Adams, Fabio; Romero, Ivana Lopes; Silva, Cely Barreto da; Manzano, Roberta Pereira de Almeida


    To analyze the antimicrobial properties of silicon oil (Óleo de Silicone®, Ophthalmos, Brazil) on in vitro bacterial growth of different microorganisms related to endophthalmitis. The following microorganisms were analyzed: (1) Pseudomonas aeruginosa (ATCC 27583); (2) Escherichia coli (ATCC 25922); (3) Staphylococcus aureus (ATCC 25923); (4) Staphylococcus epidermidis (ATCC 12228); (5) Candida albicans (ATCC 10231); (6) Klebsiella pneumoniae (ATCC 13883); and (7) Streptococcus pneumoniae (ATCC 49619). The plates were incubated at 35 ± 2ºC and its growth examined after 24 hours. An empty disk was placed in the center of each plate as a control. No inhibition halos were verified in any of the plates containing the four different concentrations of the bacterial inocula. The silicon oil 1000 cps does not have any effect on bacterial growth of any of the studied microorganisms.

  20. Evaluation of silicon oil on bacterial growth

    Directory of Open Access Journals (Sweden)

    Fabio Adams


    Full Text Available PURPOSE: To analyze the antimicrobial properties of silicon oil (Óleo de Silicone®, Ophthalmos, Brazil on in vitro bacterial growth of different microorganisms related to endophthalmitis. METHODS: The following microorganisms were analyzed: (1 Pseudomonas aeruginosa (ATCC 27583; (2 Escherichia coli (ATCC 25922; (3 Staphylococcus aureus (ATCC 25923; (4 Staphylococcus epidermidis (ATCC 12228; (5 Candida albicans (ATCC 10231; (6 Klebsiella pneumoniae (ATCC 13883; and (7 Streptococcus pneumoniae (ATCC 49619. The plates were incubated at 35 ± 2ºC and its growth examined after 24 hours. An empty disk was placed in the center of each plate as a control. RESULTS: No inhibition halos were verified in any of the plates containing the four different concentrations of the bacterial inocula. CONCLUSIONS: The silicon oil 1000 cps does not have any effect on bacterial growth of any of the studied microrganisms.

  1. Heme Synthesis and Acquisition in Bacterial Pathogens. (United States)

    Choby, Jacob E; Skaar, Eric P


    Bacterial pathogens require the iron-containing cofactor heme to cause disease. Heme is essential to the function of hemoproteins, which are involved in energy generation by the electron transport chain, detoxification of host immune effectors, and other processes. During infection, bacterial pathogens must synthesize heme or acquire heme from the host; however, host heme is sequestered in high-affinity hemoproteins. Pathogens have evolved elaborate strategies to acquire heme from host sources, particularly hemoglobin, and both heme acquisition and synthesis are important for pathogenesis. Paradoxically, excess heme is toxic to bacteria and pathogens must rely on heme detoxification strategies. Heme is a key nutrient in the struggle for survival between host and pathogen, and its study has offered significant insight into the molecular mechanisms of bacterial pathogenesis. Copyright © 2016 Elsevier Ltd. All rights reserved.

  2. Multiple bacterial species reside in chronic wounds

    DEFF Research Database (Denmark)

    Gjødsbøl, Kristine; Christensen, Jens Jørgen; Karlsmark, Tonny


    The aim of the study was to investigate the bacterial profile of chronic venous leg ulcers and the importance of the profile to ulcer development. Patients with persisting venous leg ulcers were included and followed for 8 weeks. Every second week, ulcer samples were collected and the bacterial...... of P. aeruginosa in venous leg ulcers can induce ulcer enlargement and/or cause delayed healing....... species present were identified. More than one bacterial species were detected in all the ulcers. The most common bacteria found were Staphylococcus aureus (found in 93.5% of the ulcers), Enterococcus faecalis (71.7%), Pseudomonas aeruginosa (52.2%), coagulase-negative staphylococci (45.7%), Proteus...

  3. Characterization of TEMPO-oxidized bacterial cellulose

    International Nuclear Information System (INIS)

    Nascimento, Eligenes S.; Pereira, Andre L.S.; Lima, Helder L.; Barroso, Maria K. de A.; Barros, Matheus de O.; Morais, Joao P.S.; Borges, Maria de F.; Rosa, Morsyleide de F.


    The aim of this study was to characterize the TEMPO-oxidized bacterial cellulose, as a preliminary research for further application in nanocomposites. Bacterial cellulose (BC) was selectively oxidized at C-6 carbon by TEMPO radical. Oxidized bacterial cellulose (BCOX) was characterized by TGA, FTIR, XRD, and zeta potential. BCOX suspension was stable at pH 7.0, presented a crystallinity index of 83%, in spite of 92% of BC, because of decrease in the free hydroxyl number. FTIR spectra showed characteristic BC bands and, in addition, band of carboxylic group, proving the oxidation. BCOX DTG showed, in addition to characteristic BC thermal events, a maximum degradation peak at 233 °C, related to sodium anhydro-glucuronate groups formed during the cellulose oxidation. Thus, BC can be TEMPO-oxidized without great loss in its structure and properties. (author)

  4. Bacterial Networks in Cells and Communities. (United States)

    Sourjik, Victor; Vorholt, Julia A


    Research on the bacterial regulatory networks is currently experiencing a true revival, driven by advances in methodology and by emergence of novel concepts. The biannual conference Bacterial Networks (BacNet15) held in May 2015, in Sant Feliu de Guíxols, Spain, covered progress in the studies of regulatory networks that control bacterial physiology, cell biology, stress responses, metabolism, collective behavior and evolution. It demonstrated how interdisciplinary approaches that combine molecular biology and biochemistry with the latest microscopy developments, whole cell (-omics) approaches and mathematical modeling can help understand design principles relevant in microbiology. It further showed how current biotechnology and medical microbiology could profit from our knowledge of and ability to engineer regulatory networks of bacteria. Copyright © 2015 Elsevier Ltd. All rights reserved.

  5. Mesoscopic modeling of bacterial flagellar microhydrodynamics. (United States)

    Gebremichael, Yeshitila; Ayton, Gary S; Voth, Gregory A


    A particle-based hybrid method of elastic network model and smooth-particle hydrodynamics has been employed to describe the propulsion of bacterial flagella in a viscous hydrodynamic environment. The method explicitly models the two aspects of bacterial propulsion that involve flagellar flexibility and long-range hydrodynamic interaction of low-Reynolds-number flow. The model further incorporates the molecular organization of the flagellar filament at a coarse-grained level in terms of the 11 protofilaments. Each of these protofilaments is represented by a collection of material points that represent the flagellin proteins. A computational model of a single flexible helical segment representing the filament of a bacterial flagellum is presented. The propulsive dynamics and the flow fields generated by the motion of the model filament are examined. The nature of flagellar deformation and the influence of hydrodynamics in determining the shape of deformations are examined based on the helical filament.

  6. Synthetic analogs of bacterial quorum sensors

    Energy Technology Data Exchange (ETDEWEB)

    Iyer, Rashi [Los Alamos, NM; Ganguly, Kumkum [Los Alamos, NM; Silks, Louis A [Los Alamos, NM


    Bacterial quorum-sensing molecule analogs having the following structures: ##STR00001## and methods of reducing bacterial pathogenicity, comprising providing a biological system comprising pathogenic bacteria which produce natural quorum-sensing molecule; providing a synthetic bacterial quorum-sensing molecule having the above structures and introducing the synthetic quorum-sensing molecule into the biological system comprising pathogenic bacteria. Further is provided a method of targeted delivery of an antibiotic, comprising providing a synthetic quorum-sensing molecule; chemically linking the synthetic quorum-sensing molecule to an antibiotic to produce a quorum-sensing molecule-antibiotic conjugate; and introducing the conjugate into a biological system comprising pathogenic bacteria susceptible to the antibiotic.

  7. Synthetic analogs of bacterial quorum sensors

    Energy Technology Data Exchange (ETDEWEB)

    Iyer, Rashi S.; Ganguly, Kumkum; Silks, Louis A.


    Bacterial quorum-sensing molecule analogs having the following structures: ##STR00001## and methods of reducing bacterial pathogenicity, comprising providing a biological system comprising pathogenic bacteria which produce natural quorum-sensing molecule; providing a synthetic bacterial quorum-sensing molecule having the above structures and introducing the synthetic quorum-sensing molecule into the biological system comprising pathogenic bacteria. Further is provided a method of targeted delivery of an antibiotic, comprising providing a synthetic quorum-sensing molecule; chemically linking the synthetic quorum-sensing molecule to an antibiotic to produce a quorum-sensing molecule-antibiotic conjugate; and introducing the conjugate into a biological system comprising pathogenic bacteria susceptible to the antibiotic.

  8. Determination of Bacterial Growth in Culture Media

    International Nuclear Information System (INIS)

    Elly Ellyna Rashid; Shariza Hanim Zainal Abidin; Mok, P.S.


    Bacteria is one of the important microorganism in our daily life. Bacteria provides human beings with products in the field of medical, industry, food, agriculture and others. Determination of bacteria growth is important so that we can enjoy the most benefit from it. Spread-plate method is one of the methods to obtain the bacterial counts. Agar plates, such as Nutrient Agar or Plate Count Agar are usually used for this purpose. Bacterial culture will be diluted first before being spread on the agar plate and incubated at specific temperature. The number of bacteria in colony-forming unit (CFU) will be counted the next day. The count will be used to determine the bacterial growth. (author)

  9. Bacterial Cholangitis, Cholecystitis, or both in Dogs. (United States)

    Tamborini, A; Jahns, H; McAllister, H; Kent, A; Harris, B; Procoli, F; Allenspach, K; Hall, E J; Day, M J; Watson, P J; O'Neill, E J


    Bacterial cholangitis and cholecystitis are rarely reported, poorly characterized diseases in the dog. To characterize the clinical features of these conditions. Twenty-seven client-owned dogs with bacterial cholangitis, cholecystitis, or both. Multicenter, retrospective cases series of dogs with bacterial cholangitis, cholecystitis, or both, presenting January 2000 to June 2011 to 4 Veterinary Schools in Ireland/United Kingdom. Interrogation of hospital databases identified all cases with the inclusion criteria; histopathologically confirmed cholangitis or cholecystitis and bile culture/cytology results supporting a bacterial etiology. Twenty-seven dogs met the inclusion criteria with approximately 460 hepatitis cases documented over the same study period. Typical clinical pathology findings were increases in liver enzyme activities (25/26), hyperbilirubinemia (20/26), and an inflammatory leukogram (21/24). Ultrasound findings, although nonspecific, aided decision-making in 25/26 cases. The most frequent hepatobiliary bacterial isolates were Escherichia coli (n = 17; 16 cases), Enterococcus spp. (n = 8; 6 cases), and Clostridium spp. (n = 5; 5 cases). Antimicrobial resistance was an important feature of aerobic isolates; 10/16 E. coli isolates resistant to 3 or more antimicrobial classes. Biliary tract rupture complicated nearly one third of cases, associated with significant mortality (4/8). Discharged dogs had a guarded to fair prognosis; 17/18 alive at 2 months, although 5/10 re-evaluated had persistent liver enzyme elevation 2-12 months later. Bacterial cholangitis and cholecystitis occur more frequently than suggested by current literature and should be considered in dogs presenting with jaundice and fever, abdominal pain, or an inflammatory leukogram or with ultrasonographic evidence of gallbladder abnormalities. Copyright © 2016 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of

  10. Practically delineating bacterial species with genealogical concordance. (United States)

    Venter, Stephanus N; Palmer, Marike; Beukes, Chrizelle W; Chan, Wai-Yin; Shin, Giyoon; van Zyl, Elritha; Seale, Tarren; Coutinho, Teresa A; Steenkamp, Emma T


    Bacterial species are commonly defined by applying a set of predetermined criteria, including DNA-DNA hybridization values, 16S rRNA gene sequence similarity, phenotypic data as well as genome-based criteria such as average nucleotide identity or digital DNA-DNA hybridization. These criteria mostly allow for the delimitation of taxa that resemble typical bacterial species. Their application is often complicated when the objective is to delineate new species that are characterized by significant population-level diversity or recent speciation. However, we believe that these complexities and limitations can be easily circumvented by recognizing that bacterial species represent unique and exclusive assemblages of diversity. Within such a framework, methods that account for the population processes involved in species evolution are used to infer species boundaries. A method such as genealogical concordance analysis is well suited to delineate a putative species. The existence of the new taxon is then interrogated using an array of traditional and genome-based characters. By making use of taxa in the genera Pantoea, Paraburkholderia and Escherichia we demonstrate in a step-wise process how genealogical concordance can be used to delimit a bacterial species. Genetic, phenotypic and biological criteria were used to provide independent lines of evidence for the existence of that taxon. Our six-step approach to species recognition is straightforward and applicable to bacterial species especially in the post-genomic era, with increased availability of whole genome sequences. In fact, our results indicated that a combined genome-based comparative and evolutionary approach would be the preferred alternative for delineating coherent bacterial taxa.

  11. Prediction of bacterial growth on xenobiotics

    DEFF Research Database (Denmark)

    Brock, Andreas Libonati; Kästner, Matthias; Trapp, Stefan


    thermodynamic considerations of stoichiometrically balanced reactions is typically done in biotechnology and wastewater treatment [5], an approach recently adopted by Helbling et al. [6]. More recent methods specifically incorporate detailed knowledge of the degradation pathway and bacterial metabolism......The utilisation of a given substrate leads to bacterial growth and the associated yield is normally determined experimentally. Different yield estimation methods exist based on knowledge of the Gibbs energy of reaction and the energy needed for synthesis of new biomass [1-4]. Estimating yield from...

  12. The clinical impact of bacterial biofilms

    DEFF Research Database (Denmark)

    Høiby, Niels; Ciofu, Oana; Johansen, Helle Krogh


    . Likewise, chronic Pseudomonas aeruginosa lung infections in cystic fibrosis patients are caused by biofilm growing mucoid strains. Gradients of nutrients and oxygen exist from the top to the bottom of biofilms and the bacterial cells located in nutrient poor areas have decreased metabolic activity....... Bacterial biofilms are resistant to antibiotics, disinfectant chemicals and to phagocytosis and other components of the innate and adaptive inflammatory defense system of the body. It is known, for example, that persistence of staphylococcal infections related to foreign bodies is due to biofilm formation...

  13. Impact of hydrodynamic stresses on bacterial flagella (United States)

    Das, Debasish; Riley, Emily; Lauga, Eric


    The locomotion of bacteria powered by helical filaments, such as Escherichia coli, critically involves the generation of flows and hydrodynamic stresses which lead to forces and moments balanced by the moment applied by the bacterial rotary motor (which is embedded in the cell wall) and the deformation of the short flexible hook. In this talk we use numerical computations to accurately compute these hydrodynamic stresses, to show how they critically lead to fluid-structure instabilities at the whole-cell level, and enquire if they can be used to rationalise experimental measurements of bacterial motor torques. ERC Consolidator Grant.

  14. Tuning bacterial hydrodynamics with magnetic fields (United States)

    Pierce, C. J.; Mumper, E.; Brown, E. E.; Brangham, J. T.; Lower, B. H.; Lower, S. K.; Yang, F. Y.; Sooryakumar, R.


    Magnetotactic bacteria are a group of motile prokaryotes that synthesize chains of lipid-bound, magnetic nanoparticles called magnetosomes. This study exploits their innate magnetism to investigate previously unexplored facets of bacterial hydrodynamics at surfaces. Through use of weak, uniform, external magnetic fields and local, micromagnetic surface patterns, the relative strength of hydrodynamic, magnetic, and flagellar force components is tuned through magnetic control of the bacteria's orientation. The resulting swimming behaviors provide a means to experimentally determine hydrodynamic parameters and offer a high degree of control over large numbers of living microscopic entities. The implications of this controlled motion for studies of bacterial motility near surfaces and for micro- and nanotechnology are discussed.

  15. Carbon nanotubes as anti-bacterial agents. (United States)

    Mocan, Teodora; Matea, Cristian T; Pop, Teodora; Mosteanu, Ofelia; Buzoianu, Anca Dana; Suciu, Soimita; Puia, Cosmin; Zdrehus, Claudiu; Iancu, Cornel; Mocan, Lucian


    Multidrug-resistant bacterial infections that have evolved via natural selection have increased alarmingly at a global level. Thus, there is a strong need for the development of novel antibiotics for the treatment of these infections. Functionalized carbon nanotubes through their unique properties hold great promise in the fight against multidrug-resistant bacterial infections. This new family of nanovectors for therapeutic delivery proved to be innovative and efficient for the transport and cellular translocation of therapeutic molecules. The current review examines the latest progress in the antibacterial activity of carbon nanotubes and their composites.

  16. CT scan of bacterial and aseptic meningitis

    International Nuclear Information System (INIS)

    Takemoto, Kazumasa; Saiwai, Shigeo; Tamaoka, Koichi


    CT scans of the patients with aseptic and bacterial meningitis were reviewed and compared to previous reports. In aseptic meningitis, no abnormal CT findings were observed. In bacterial meningitis, CT findings were ventricular dilatation, subdural fluid collection, parenchymal low density, intracerebral hematoma and meningeal enhancement after contrast injection. Three patients among 48 suffered from status epileptics during the course of the illness. All of 3 patients developed parenchymal inhomogeneous low density and progressive ventricular dilatation which did not improve after ventricular peritoneal shunt surgery. We believe that these changes are most likely due to hypoxic hypoxemia during epileptic seizure and meningitis itself seems to play a little role. (author)

  17. Neurosonographic findings of bacterial meningitis in Infants

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Moon Chul; Lee, Sung Sik; Lee, Hong Kue; Lee, Soon Il [Sowa Children' s Hospital, Seoul (Korea, Republic of)


    44 infants under 1 year were studied retrospectively during these illness and follow up after 1 week intervals. The spectrum of sonographic features of bacterial meningitis in acute stage included normal scan (20 patients), echogenic sulci (10 patients), echogenic lining of epandymas (8 patients), Abnormal parenchymal echogenecity (6 patients). On follow up examination with 1 week intervals, variety of complications was found in 14 patients (32%) of the infants. There were ventriculomegaly in 7 patients, extraaxial fluid collection in 4 patients, brain abscess in 2 patients and poor encephalic cyst in 1 patient. We conclude that ultrasound was an effective method for evaluation of progression and complications of bacterial meningitis.

  18. CT scan of bacterial and aseptic meningitis

    Energy Technology Data Exchange (ETDEWEB)

    Takemoto, Kazumasa; Saiwai, Shigeo; Tamaoka, Koichi (Kobe Central Municipal Hospital (Japan))


    CT scans of the patients with aseptic and bacterial meningitis were reviewed and compared to previous reports. In aseptic meningitis, no abnormal CT findings were observed. In bacterial meningitis, CT findings were ventricular dilatation, subdural fluid collection, parenchymal low density, intracerebral hematoma and meningeal enhancement after contrast injection. Three patients among 48 suffered from status epileptics during the course of the illness. All of the 3 patients developed parenchymal inhomogeneous low density and progressive ventricular dilatation which did not improve after ventricular peritoneal shunt surgery. We believe that these changes are most likely due to hypoxic hypoxemia during epileptic seizure and meningitis itself seems to play a little role.

  19. Bacterial subversion of host innate immune pathways. (United States)

    Baxt, Leigh A; Garza-Mayers, Anna Cristina; Goldberg, Marcia B


    The pathogenesis of infection is a continuously evolving battle between the human host and the infecting microbe. The past decade has brought a burst of insights into the molecular mechanisms of innate immune responses to bacterial pathogens. In parallel, multiple specific mechanisms by which microorganisms subvert these host responses have been uncovered. This Review highlights recently characterized mechanisms by which bacterial pathogens avoid killing by innate host responses, including autophagy pathways and a proinflammatory cytokine transcriptional response, and by the manipulation of vesicular trafficking to avoid the toxicity of lysosomal enzymes.

  20. Enzymatic removal and disinfection of bacterial biofilms

    DEFF Research Database (Denmark)

    Johansen, Charlotte; Falholt, Per; Gram, Lone


    -coated hydroxyapatite. The activity of enzymes against bacterial cells in biofilm was measured by fluorescence microscopy and an indirect conductance test in which evolution of carbon dioxide was measured. Glucose oxidase combined with lactoperoxidase was bactericidal against biofilm bacteria but did not remove...... the biofilm from the substrata. A complex mixture of polysaccharide-hydrolyzing enzymes was able to remove bacterial biofilm from steel and polypropylene substrata but did not have a significant bactericidal activity. Combining oxidoreductases with polysaccharide-hydrolyzing enzymes resulted in bactericidal...... activity as well as removal of the biofilm...

  1. Etude des transformations de phase dans les alliages de titane, 2; contribution à l'étude des transformation de l'alliage de titane TA6 V6 E2 en conditions isothermes après trempe à partir du domaine $(\\alpha + \\beta)$

    CERN Document Server

    Fontalirand, C; Fidelle, J P; Hocheid, B; Beauvais, C no 1; Fidelle, J P no 2; Hocheid, B no 2; Fontalirand, C no 1


    Etude des transformations de phase dans les alliages de titane, 2; contribution à l'étude des transformation de l'alliage de titane TA6 V6 E2 en conditions isothermes après trempe à partir du domaine $(\\alpha + \\beta)$

  2. Etude des phenomenes de penetration des especes chimiques dans les revetements cathodiques des cuves d'electrolyse de l'aluminium (United States)

    Brisson, Pierre-Yves

    Cette these decrit l'etude effectuee sur les phenomenes d'insertion du sodium et de penetration du bain electrolytique dans les revetements cathodiques des cuves d'electrolyse de l'aluminium. L'etude fut effectuee a l'aide d'un montage permettant d'effectuer l'electrolyse en laboratoire. Trois types de revetement cathodiques furent etudies : des blocs semi-graphitique, des blocs graphitiques et des blocs graphitises. L'insertion du sodium dans les trois differents types de blocs fut etudiee a l'aide de la spectrometrie des photoelectrons X (XPS). L'analyse a permis de mettre en evidence deux formes de sodium dans l'anthracite et la phase liante des materiaux semi-graphitiques et graphitiques, indiquant qu'une fraction du sodium se retrouve sous forme adsorbee dans les micropores des materiaux alors que l'autre fraction est inseree dans la structure cristalline du carbone. Dans les phases graphitiques (materiaux graphitises et graphitiques), seuls les micropores sont occupes par le sodium Ce resultat explique la tendance observee selon laquelle l'ajout de graphite dans les blocs permet d'abaisser le gonflement sodique. Les mecanismes de penetration du bain dans le reseau poreux des materiaux furent etudies en microscopie electronique et en diffraction des rayons X sur des echantillons apres differents temps d'electrolyses et en variant l'atmosphere (soit sous argon ou sous azote). Ces analyses ont permis d'identifier le mecanisme conduisant a la mouillabilite du bain sur le carbone en fonction de l'atmosphere entourant l'electrolyse. Ainsi, sous azote, la formation de NaCN dans les pores des materiaux par reactions entre le sodium et l'azote permet une mouillabilite accrue du bain alors qu'en absence d'azote (sous argon), le carbure d'aluminium, formes a la surface des pores, joue un role similaire. Dans ce dernier cas, la penetration du bain est moins rapide etant donnee la necessite de toujours amener des especes contenant de l'aluminium en tete du front de

  3. Clinical and symptomatological study of pigs subjected to a lethal dose of integral gamma irradiation; Etude clinique et symptomatologique chez le porc soumis a une irradiation gamma totale a dose letale

    Energy Technology Data Exchange (ETDEWEB)

    Vaiman, M.; Guenet, J.-L.; Maas, J.; Nizza, P


    Results are reported from a clinical and haematological study on a Corsican species of pigs wholly exposed to an approximately lethal dose of {gamma} radiation. The aim of this work was to examine the changes in the irradiation syndrome of irradiation for pigs to make it thus possible to devise further experiments, in particular in the therapeutic field. The dose received was 285 rads (measured as the absorption in the vertical antero-posterior medial plane). Data are presented on cyto-haematological changes in the blood circulating immediately after irradiation, and followed up to death, and changes in the medullary cytology after irradiation. The clinical picture of lethal radiation injury in swine is described. (authors) [French] Les auteurs rapportent les resultats d'une etude clinique et hematologique chez des porcs de race corse irradies in toto a dose sensiblement letale. Le but de cette etude etait de connaitre l'evolution du syndrome aigu d'irradiation chez le porc et de permettre ainsi le developpement d'experimentations ulterieures, en particulier dans le domaine therapeutique. La dose delivree etait de 285 rad (en dose absorbee au niveau du plan median vertical anteroposterieur. L'etude a porte essentiellement: 1. Sur les modifications cyclo-hematologiques du sang circulant immediatement apres l'irradiation, pour les differentes lignees cellulaires; l'evolution de ces modifications a ete notee jusqu'a la mort; 2. Sur les modifications de la cytologie medullaire apres irradiation (evolution du myelogramme et essai d'evaluation de la cellularite de la moelle osseuse);: 3. Sur les signes cliniques, d'ailleurs tres discrets, observes chez les porcs apres irradiation. (auteurs)

  4. Rifaximin has minor effects on bacterial composition, inflammation and bacterial translocation in cirrhosis

    DEFF Research Database (Denmark)

    Kimer, Nina; Pedersen, Julie S.; Tavenier, Juliette


    .4), and MELD score 12 (±3.9). Patients received rifaximin 550 mg BD (n=36) or placebo BD (n=18). Blood and faecal (n=15) sampling were conducted at baseline and after four weeks. Bacterial DNA in blood was determined by real-time qPCR 16S rRNA gene quantification. Bacterial composition in faeces was analysed......: Four weeks of treatment with rifaximin had no impact on the inflammatory state and only minor effects on BT and intestinal bacterial composition in stable, decompensated cirrhosis (NCT01769040)....

  5. Gamma-irradiated bacterial preparation having anti-tumor activity

    International Nuclear Information System (INIS)

    Vass, A.A.; Tyndall, R.L.; Terzaghi-Howe, P.


    This application describes a bacterial preparation from Pseudomonas species isolated number s ign15 ATCC 55638 that has been exposed to gamma radiation exhibits cytotoxicity that is specific for neoplastic carcinoma cells. A method for obtaining a bacterial preparation having antitumor activity consists of suspending a bacterial isolate in media and exposing the suspension to gamma radiation. A bacterial preparation of an aged culture of an amoeba-associated bacteria exhibits anti-reverse transcriptase activity. A method for obtaining a bacterial preparation having anti-reverse transcriptase activity from an amoeba-associated bacterial isolate grown to stationary phase is disclosed

  6. Datos experimentales de la cinética del secado y del modelo matemático para pulpa de cupuaçu (Theobroma grandiflorum en rodajas Kinetic drying experimental data and mathematical model for cupuaçu pulp (Theobroma grandiflora slices

    Directory of Open Access Journals (Sweden)

    Abraham Damian Giraldo-Zuniga


    Full Text Available En este trabajo fueron obtenidos experimentalmente la cinética del secado de la pulpa de cupuaçu en forma de rodajas con espesor de 0,5 cm. El secado fue realizado utilizándose un secador laboratorial de bandejas, a las temperaturas de 50, 60 y 70 ºC y a la velocidad del aire de secado de 1,5 m/s. De acuerdo con los datos de la cinética del secado se puede observar que cuanto mayor es la temperatura del secado mayor es la velocidad del secado. El tiempo requerido para secar el producto hasta una humedad del 20%, fueron 9,2, 8,1 y 7,3 horas para las temperaturas de secado de 50, 60 y 70 ºC respectivamente. Las curvas experimentales del secado fueron ajustados al modelo difusional de Fick considerándose la muestra como una lámina infinita y al modelo de Page. Los dos modelos se ajustaron bien a los datos experimentales. Los coeficientes de difusión del modelo de Fick variaron de 1.171 a 1.561 m/s².This work examined experimentally the kinetics of drying cupuaçu pulp (Theobroma grandiflorum slices. The experimental runs were driven in a laboratory dryer, at temperatures of 50, 60 and 70 ºC and an air drying velocity of 1.5 m/s. According to the kinetics study, increasing the temperature led to a decrease in drying time. The required drying times were 9.2, 8.1 and 7.3 hours for drying temperatures of 50, 60 and 70 ºC respectively. The experimental drying curves were adjusted to Fick's diffusional model for an infinite lamina with shrinkage and Page model. The diffusion coefficients of the Fick model varied between 1.171 and 1.561 m/s².

  7. Bacterial Acclimation Inside an Aqueous Battery. (United States)

    Dong, Dexian; Chen, Baoling; Chen, P


    Specific environmental stresses may lead to induced genomic instability in bacteria, generating beneficial mutants and potentially accelerating the breeding of industrial microorganisms. The environmental stresses inside the aqueous battery may be derived from such conditions as ion shuttle, pH gradient, free radical reaction and electric field. In most industrial and medical applications, electric fields and direct currents are used to kill bacteria and yeast. However, the present study focused on increasing bacterial survival inside an operating battery. Using a bacterial acclimation strategy, both Escherichia coli and Bacillus subtilis were acclimated for 10 battery operation cycles and survived in the battery for over 3 days. The acclimated bacteria changed in cell shape, growth rate and colony color. Further analysis indicated that electrolyte concentration could be one of the major factors determining bacterial survival inside an aqueous battery. The acclimation process significantly improved the viability of both bacteria E. coli and B. subtilis. The viability of acclimated strains was not affected under battery cycle conditions of 0.18-0.80 mA cm(-2) and 1.4-2.1 V. Bacterial addition within 1.0×10(10) cells mL(-1) did not significantly affect battery performance. Because the environmental stress inside the aqueous battery is specific, the use of this battery acclimation strategy may be of great potential for the breeding of industrial microorganisms.

  8. Childhood bacterial meningitis in Mbarara Hospital, Uganda ...

    African Journals Online (AJOL)

    Background : The recommended antibiotic treatment of bacterial meningitis has come under scrutiny following frequent reports of in-vitro resistance by the common causative organisms to penicillin and chloramphenicol. Objective : The study recorded the causative organisms, antibiotic sensitivity patterns and outcome of ...

  9. Computed Tomography Study Of Complicated Bacterial Meningitis ...

    African Journals Online (AJOL)

    To monitor the structural intracranial complications of bacterial meningitis using computed tomography (CT) scan. Retrospective study of medical and radiological records of patients who underwent CT scan over a 4 year period. AUniversityTeachingHospital in a developing country. Thirty three patients with clinically and ...

  10. The normal bacterial flora prevents GI disease

    Indian Academy of Sciences (India)

    First page Back Continue Last page Overview Graphics. The normal bacterial flora prevents GI disease. Inhibits pathogenic enteric bacteria. Decrease luminal pH; Secrete bacteriocidal proteins; Colonization resistance; Block epithelial binding – induce MUC2. Improves epithelial and mucosal barrier integrity. Produce ...

  11. Horizontal gene transfer and bacterial diversity

    Indian Academy of Sciences (India)


    reservoir; under Antarctic ice or in near-boiling water; in acid springs or in alkaline pools – microbial life exists .... The close similarity of the rrnE region of Salmo- nella subspecies I to that of E. coli suggests lateral ..... transfer and the nature of bacterial innovation; Nature (Lon- don) 405 299–304. Pan A, Dutta C and Das J ...

  12. Childhood bacterial meningitis in Mbarara Hospital, Uganda ...

    African Journals Online (AJOL)

    Background. The recommended antibiotic treatment of bacterial meningitis has come under scrutiny following frequent reports of in-vitro resistance by the common causative organisms to penicillin and chloramphenicol. Objective. The study recorded'the causative organisms, antibiotic sensitivity patterns and outcome of ...

  13. Bacterial Cell Wall Growth, Shape and Division

    NARCIS (Netherlands)

    Derouaux, A.; Terrak, M.; den Blaauwen, T.; Vollmer, W.; Remaut, H.; Fronzes, R.


    The shape of a bacterial cell is maintained by its peptidoglycan sacculus that completely surrounds the cytoplasmic membrane. During growth the sacculus is enlarged by peptidoglycan synthesis complexes that are controlled by components linked to the cytoskeleton and, in Gram-negative bacteria, by

  14. Methacrylate hydrogels reinforced with bacterial cellulose

    Czech Academy of Sciences Publication Activity Database

    Hobzová, Radka; Dušková-Smrčková, Miroslava; Michálek, Jiří; Karpushkin, Evgeny; Gatenholm, P.


    Roč. 61, č. 7 (2012), s. 1193-1201 ISSN 0959-8103 R&D Projects: GA AV ČR KJB400500902 Institutional research plan: CEZ:AV0Z40500505 Keywords : bacterial cellulose * methacrylate hydrogel * composite Subject RIV: CD - Macromolecular Chemistry Impact factor: 2.125, year: 2012


    African Journals Online (AJOL)


    Key Words: Bacterial wilt, enset, survival, transmission, Xanthomonas campestris pv. musacearum. RÉSUMÉ. La transmission .... than young plants. A 100% disease incidence was recorded at 60 days after inoculation on plants inoculated at 6 months after transplanting. Plants inoculated at. 6 months after transplanting had ...

  16. Respiratory bacterial infections in cystic fibrosis

    DEFF Research Database (Denmark)

    Ciofu, Oana; Hansen, Christine R; Høiby, Niels


    PURPOSE OF REVIEW: Bacterial respiratory infections are the main cause of morbidity and mortality in patients with cystic fibrosis (CF). Pseudomonas aeruginosa remains the main pathogen in adults, but other Gram-negative bacteria such as Achromobacter xylosoxidans and Stenotrophomonas maltophilia...

  17. Bacterial Diversity across Individual Lichens▿ † (United States)

    Mushegian, Alexandra A.; Peterson, Celeste N.; Baker, Christopher C. M.; Pringle, Anne


    Symbioses are unique habitats for bacteria. We surveyed the spatial diversity of bacterial communities across multiple individuals of closely related lichens using terminal restriction fragment length polymorphism (T-RFLP) and pyrosequencing. Centers of lichens house richer, more consistent assemblages than species-poor and compositionally disparate lichen edges, suggesting that ecological succession plays a role in structuring these communities. PMID:21531831

  18. Seizures in adults with bacterial meningitis

    NARCIS (Netherlands)

    Zoons, E.; Weisfelt, M.; de Gans, J.; Spanjaard, L.; Koelman, J. H. T. M.; Reitsma, J. B.; van de Beek, D.


    Objective: To evaluate the occurrence and prognostic relevance of seizures in adults with community-acquired bacterial meningitis. Methods: An observational cross-sectional study, in which patients with seizures are selected from a prospective nationwide cohort of 696 episodes of community-acquired

  19. Advances in treatment of bacterial meningitis

    NARCIS (Netherlands)

    van de Beek, Diederik; Brouwer, Matthijs C.; Thwaites, Guy E.; Tunkel, Allan R.


    Bacterial meningitis kills or maims about a fifth of people with the disease. Early antibiotic treatment improves outcomes, but the effectiveness of widely available antibiotics is threatened by global emergence of multidrug-resistant bacteria. New antibiotics, such as fluoroquinolones, could have a

  20. Editorial: Spontaneous bacterial peritonitis | Lule | East African ...

    African Journals Online (AJOL)

    Journal Home > Vol 81, No 3 (2004) >. Log in or Register to get access to full text downloads. Username, Password, Remember me, or Register. Editorial: Spontaneous bacterial peritonitis. GN Lule. Abstract. No Abstract Available. Full Text: EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT ...