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Sample records for bacteriana por lactococcus

  1. Vaginosis bacteriana por Gardnerella vaginalis: Nuevas enseñanzas desde la ecología molecular

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    Andrés Zúñiga; Fabián Tobar-Tosse

    2015-01-01

    La vaginosis bacteriana (VB), es la afección vaginal más frecuente en las mujeres en edad reproductiva generada por un desbalance en el ecosistema vaginal que ocasiona complicaciones severas para la salud reproductiva. Existen hipótesis de origen biológico que relacionan la presencia de organismos como Gardnerella vaginalis, Prevotella sp, Atopobium vaginae como la causa más frecuente relacionada con la vaginosis, los cuales logran desplazar poblacionalmente microorganismos con capacidad prot...

  2. Infecciones por bacterias poco comunes y oncogénesis bacteriana

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    Horacio A. Lopardo

    2014-01-01

    Full Text Available La recuperación de algunos microorganismos de aislamiento esporádico en el laboratorio de microbiología clínica podría significar la existencia de algún defecto inmunitarioespecial en el paciente. Por ejemplo, se ha descrito una importante correlación entre Clostridium septicum y carcinoma de colon, y se han visto relacionadas con leucemias y linfomas a especies que aparecen casi siempre como contaminantes (Bacillus spp., Corynebacterium spp. y a otras raramente aisladas en otros contextos (Capnocytophaga spp.. Hay bacterias que se aíslan casi exclusivamente de pacientes con sida (Rhodococcus equi. Se ha observado una mayor frecuencia de infecciones por Campylobacter spp., Aeromonas spp. y estreptococos del grupo G y del grupo mitis en individuos con algún tipo de cáncer que en el resto de los pacientes. También hay bacterias que son marcadoras de algún cáncer no detectado o que afectan más a pacientes neutropénicos que a individuos normoinmunes. La alteración de la reacción inflamatoria, la linfoproliferación mediada por antígenos bacterianos y la inducción de hormonas que aumentan la proliferación de las células epiteliales podrían ser causas de la oncogénesis bacteriana. Los ejemplos clásicos son el adenocarcinoma gástrico inducido por Helicobacter pylori, la asociación de la bacteriemia por estreptococos del grupo bovis y el cáncer de colon y los linfomas de tejido linfoide asociado a mucosas (MALT en vinculación con especies de Helicobacter (MALT gástricos y con Chlamydophila spp. (MALT oculares. El aislamiento de alguno de estos patógenos debería ser un llamado de atención para inducir al estudio de alguna enfermedad maligna.

  3. Vaginosis bacteriana por Gardnerella vaginalis: Nuevas enseñanzas desde la ecología molecular

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    Andrés Zúñiga

    2015-06-01

    Full Text Available La vaginosis bacteriana (VB, es la afección vaginal más frecuente en las mujeres en edad reproductiva generada por un desbalance en el ecosistema vaginal que ocasiona complicaciones severas para la salud reproductiva. Existen hipótesis de origen biológico que relacionan la presencia de organismos como Gardnerella vaginalis, Prevotella sp, Atopobium vaginae como la causa más frecuente relacionada con la vaginosis, los cuales logran desplazar poblacionalmente microorganismos con capacidad protectora del epitelio vaginal como Lactobacillus crispatus y Lactobacillus jensenii. En la actualidad y de acuerdo a la OMS, la vaginosis bacteriana estaría implicada en alteraciones durante el embarazo como parto pre termino, bajo peso al nacer, corioamnionitis, ruptura prematura de membranas (RPM, endometritis post parto, entre otras. En los últimos años, con base estudios apoyados en datos de patrones moleculares, así como tecnología de análisis de genomas, surge una visión mucho más completa de condiciones ecológicas y agentes participantes en la vaginosis bacteriana.

  4. Tratamiento de agrandamiento gingival inducido por fenitoina asociado a placa bacteriana

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    Carmen Julia Rovira

    2013-10-01

    Full Text Available ResumenLa ingesta de algunos medicamentos puede producir un sobre crecimiento de las encías. Se reportan medicamentos tales como los bloqueadores de los canales de calcio, inmunosupresores, anticonvulsivantes que producen estos efectos adversos. Múltiples casos de agrandamientos gingivales están asociados a la ingesta de Fenitoína. El tratamiento de los agrandamientos gingivales inducidos por drogas por lo general es quirúrgico, pero mantener un buen control de placa bacteriana puede evitar en muchos casos que se presente un exagerado aumento de volumen gingival. Se presenta un caso de agrandamiento gingival en paciente masculino de 22 años de edad, bajo tratamiento antiepiléptico con fenitoína. Al examen clínico se observó aumento del volumen gingival de forma generalizada, en maxilar superior e inferior, predominante en sector anterior, produciendo ardor y sangrado durante el cepillado y la ingestión de alimentos. Al sondaje presenta múltiples pseudobolsas en los diferentes cuadrantes. La radiografía panorámica reveló pérdida ósea moderada horizontal, predominante en el sector posterosuperior, posteroinferior y anteroinferior. El plan de tratamiento consistió en fase higiénica, instrucción y motivación en higiene oral a la madre, gingivectomía y gingivoplastía en ambos maxilares. (DUAZARY 2011 No. 2, 226 - 231Abstract The importance of endodontic treatment is to achieve a complete filling and sealing of the root canal to prevent the transport of contaminants to and from root canal, with materials compatible with the surrounding tissues. Occasionally a situation may arise in which the purpose of the maintenance of working length is compromised such as overenlargement. This situation allows the extrusion of filling materials, mainly sealer cements, causing post treatment complications such as inflammatory reactions, injury to adjacent structures (orbital pain, inferior alveolar nerve injury, headache, etc., and infections

  5. Controle biológico da murcha bacteriana do tomateiro, por Pseudomonas spp. fluorescentes Biological control of bacterial wilt of tomato by fluorescent Pseudomonas spp

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    Ana Rosa Peixoto

    1997-01-01

    Esta revisão bibliográfica teve como objetivo avaliar o potencial de antagonismo de espécies de Pseudomonas fluorescentes a Pseudomonas solanacearum, agente causal da murcha bacteriana do tomateiro. Devido a dificuldade encontrada nas estratégias utilizadas para o controle da Murcha Bacteriana por meio de métodos convencionais, alguns outros tem sido estudados, como o uso de microrganismos benéficos. As rizobactérias vem proporcionando solução viável a algumas doenças consideradas de difícil ...

  6. Characterization of bacteriocins produced by Lactococcus lactis strains Caracterização de bacteriocinas produzidas por linhagens de Lactococcus lactis

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    Izildinha Moreno

    2000-09-01

    Full Text Available Bacteriocins produced by fifteen strains of Lactococcus lactis (14 L. lactis subsp. lactis and one L. lactis subsp. cremoris were heat resistant, sensitive to several proteolytic enzymes and active over a wide range of pH. Their resistance to the heating was greatly influenced by the pH. Only the strain L. lactis subsp. lactis ITAL 383 produced a bacteriocin with a wide activity spectrum, similar to nisin of L. lactis subsp. lactis ATCC 11454. This bacteriocin inhibited closely related species and other Gram-positive microorganisms including Listeria monocytogenes and Staphylococcus aureus, but it was not active against the Gram-negative bacteria tested. The identification of partially purified antimicrobial compounds by SDS-PAGE showed that bacteriocin produced by strain ITAL 383 had the same molecular weight of nisin produced by L. lactis subsp. lactis ATCC 11454.Bacteriocinas resistentes ao aquecimento produzidas por quinze linhagens de Lactococcus lactis (14 L. lactis subsp. lactis e 1 L. lactis subsp. cremoris foram sensíveis à enzimas proteolíticas e ativas em uma ampla faixa de pH. A resistência dessas bacteriocinas ao aquecimento foi fortemente influenciada pelo pH do meio. Somente a linhagem L. lactis subsp. lactis ITAL 383 produziu uma bacteriocina com um amplo espectro de atividade, semelhante ao da nisina de L. lactis subsp. lactis ATCC 11454. Esta bacteriocina inibiu as espécies relacionadas e outros microorganismos gram-positivos, inclusive Listeria monocytogenes e Staphylococcus aureus, mas não as bactérias Gram-negativas examinadas. A identificação do composto antimicrobiano parcialmente purificado por SDS-PAGE revelou um peso molecular similar entre a bacteriocina ITAL 383 e a nisina de L. lactis subsp lactis ATCC 11454.

  7. Infecciones por bacterias poco comunes y oncogénesis bacteriana

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    Horacio A. Lopardo

    2014-01-01

    La recuperación de algunos microorganismos de aislamiento esporádico en el laboratorio de microbiología clínica podría significar la existencia de algún defecto inmunitarioespecial en el paciente. Por ejemplo, se ha descrito una importante correlación entre Clostridium septicum y carcinoma de colon, y se han visto relacionadas con leucemias y linfomas a especies que aparecen casi siempre como contaminantes (Bacillus spp., Corynebacterium spp.) y a otras raramente aisladas en otros contextos (...

  8. Contextualización de las zoonosis bacterianas y virales transmitidas por roedores

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    Juan David Rodas G

    2010-04-01

    Full Text Available Hace un poco más de 3 años, registramos dentro del postgrado de biología de la Universidad de Antioquia, la línea de investigación en zoonosis emergentes y reemergentes; motivados por los, en aquel entonces, recientes eventos, que aparentemente involucraban la transmisión de Hantavirus desde roedores (1,2, e inspirado por la experiencia recién adquirida (finales del 2004, en investigación de Arenavirus y otros virus asociados con fiebres hemorrágica en laboratorios de Estados Unidos (Universidad de Wisconsin e Instituto de Virología Humana en Baltimore, Maryland (3, nos vimos estimulados a formular y proponer, en convenio con el Instituto Colombiano de Medicina Tropical (ICMT-CES, uno de los primeros estudios de Leptospirosis en roedores urbanos en Medellín (4, y más tarde, también con el ICMT y el laboratorio de Inmuno-virología, el estudio de Hantavirus, Arenavirus y Leptospira en roedores urbanos y silvestres y en humanos de la región de Urabá en Antioquia.

  9. La nueva especie bacteriana descubierta por el SERIDA, "Pseudomonas asturiensis" es patógena en soja

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    González, A.J. (Ana); Fernández-Sanz, A.M. (Ana)

    2014-01-01

    Los daños producidos en un cultivo de soja forrajera nos han llevado a describir una nueva especie bacteriana a la que hemos denominado Pseudomonas asturiensis. La bacteria se ha encontrado en diferentes localidades de Asturias y en diferentes huéspedes que incluyen malas hierbas y será necesario ampliar su estudio para conocer su potencial peligrosidad para el campo astur.

  10. Estudio de los efectos causados por la introducción de sistemas de rizorremediación en las poblaciones bacterianas nativas de un suelo contaminado por PCBs

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    Aguirre de Cárcer, Daniel

    2014-01-01

    Los diferentes análisis llevados a cabo en este estudio sostienen que la comunidad bacteriana de la rizosfera, que evolucionó de la población nativa de un suelo con un historial de contaminación por bifenilos policlorados, durante el desarrollo de las raíces de Salix vimminalis, fue diferente de la comunidad parental tanto a niveles funcionales como estructurales. La rizosfera estuvo enriquecida en especies de Proteobacteria, y en genes de ISP altamente relacionados con el grupo de las bacte...

  11. Avaliação da resistência do eucalipto à murcha-bacteriana causada por Ralstonia solanacearum

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    Reginaldo Gonçalves Mafia; Acelino Couto Alfenas; Maria Alves Ferreira

    2014-01-01

    O plantio de clones de eucalipto resistentes constitui a principal estratégia para o controle de doenças no campo. Assim, este trabalho objetivou testar métodos de inoculação de Ralstonia solanacearum visando selecionar eucalipto resistente à murcha-bacteriana. Os métodos de inoculação foram selecionados em função da facilidade operacional e testados na avaliação de quatro clones (híbridos E. urophylla x E. grandis): i) aplicação de 5 mL de inóculo (10(8) ufc/mL) na região do coleto de mudas;...

  12. Rhizosphere bacterial communities of potato cultivars evaluated through PCR-DGGE profiles Comunidades bacterianas associadas à rizosfera de cultivares de batata avaliadas por perfis de PCR-DGGE

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    Enderson Petrônio de Brito Ferreira

    2008-05-01

    ês períodos amostrais e cinco repetições. As amostras de rizosfera foram coletadas em três diferentes épocas durante o desenvolvimento das plantas. O DNA dos microrganismos associados à rizosfera foi extraído, amplificado por PCR com uso de iniciadores universais para bactérias e analisados por DGGE. Foram observadas alterações, relacionadas à cultivar e à idade da planta, nos perfis das comunidades bacterianas associadas à rizosfera das diferentes cultivares. As diferenças entre as comunidades bacterianas foram maiores na fase inicial do crescimento das plantas, com tendência a diminuir no estágio final de desenvolvimento. Essa variação foi detectada na comunidade bacteriana das cinco cultivares estudadas. A caracterização da microbiota do solo pode ser parte de programas de melhoramento de plantas a ser utilizada em estudos de avaliação de risco ambiental de batatas geneticamente modificadas.

  13. Controle biológico da murcha bacteriana do tomateiro, por Pseudomonas spp. fluorescentes Biological control of bacterial wilt of tomato by fluorescent Pseudomonas spp

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    Ana Rosa Peixoto

    1997-03-01

    Full Text Available Esta revisão bibliográfica teve como objetivo avaliar o potencial de antagonismo de espécies de Pseudomonas fluorescentes a Pseudomonas solanacearum, agente causal da murcha bacteriana do tomateiro. Devido a dificuldade encontrada nas estratégias utilizadas para o controle da Murcha Bacteriana por meio de métodos convencionais, alguns outros tem sido estudados, como o uso de microrganismos benéficos. As rizobactérias vem proporcionando solução viável a algumas doenças consideradas de difícil manejo. Dentre os mecanismos que tem sido sugeridos para o controle microbiano de patógenos de plantas, através do uso de rizobactérias fluorescentes, citamse produção de antibióticos, bactericinas, enzimas titicas, competição por espaço e nutrientes. Possuem uma alta capacidade de colonização e sobrevivência no hospedeiro, falares que são importantes no estabelecimento e introdução de microrganismos na rizosfera. Estas bactérias podem também incitar um aumento no desenvolvimento e na produção do hospedeiro, sendo denominadas de rizobactérias promotoras de crescimento de plantas.This literature review has the objetive of evaluating the antagonism potential of species of florescem Pseudomonas to Pseudomonas solanacearum which is the causal agent of bacterial wilt on the tomato crop. Due to serious limitation in the ejficiency of conventional methods of contrai, other strategies have been siudied, such as the use o/beneficiai microrganisms. Rhizobacteria have shown to be a viable alternative in the contrai of some diseases of difficult managmenl. Among the mechanisms which have been suggestedfor microbian control of plantpathogens with fluorescent rhizobacteria, can be used antibiotic production, bacteriocin, uric emimes and competition for colonization and survival capacity on the host. The survival capacity is very important in the introduction and stablishment ofthe microrganisms in the rhizosphere. These bacteria can aiso

  14. Pré-condicionamento isquêmico em diferentes tempos e seu efeito na translocação bacteriana induzida por isquemia e reperfusão intestinal em ratos

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    Aldo da Cunha Medeiros

    2013-02-01

    Full Text Available OBJETIVO: avaliar os efeitos de diferentes tempos de pré-condicionamento isquêmico(PCI intestinal sobre a translocação bacteriana (TB. MÉTODOS: Trinta ratos Wistar pesando 280±27g foram alocados em cinco grupos. No grupo IR (n=6, foi realizada laparotomia e a artéria mesentérica superior foi ocluída por microclampe atraumático por 30 minutos. Nos quatro grupos com pré-condicionamento (n=6 cada, antes dos 30 minutos de isquemia-reperfusão (I/R os ratos foram submetidos a PCI de dois, cinco, dez e 15 minutos e, em seguida, ao mesmo tempo de reperfusão. Vinte e quatro horas após, para avaliar se os tempos de pré-condicionamento influenciam o aparecimento de translocação bacteriana, amostras de linfonodos mesentéricos, fígado e baço foram coletadas em condições estéreis, para quantificação de unidades formadoras de colônias bacterianas por grama de tecido (UFC/g. Sangue foi coletado para dosagem de citocinas. RESULTADOS: No grupo I/R, o total de UFC/g em linfonodos mesentéricos, baço, fígado, bem como, a dosagem sérica de TNF-a, IL-1b e IL-6 foram significativamente maiores do que nos demais grupos (p<0,05. Pré-condicionamento de 15 minutos atenuou significativamente a BT e as citocinas séricas, comparando com os outros tempos de pré-condicionamento (p<0,05. CONCLUSÃO: Nossos dados sugerem o pré-condicionamento como fator-chave para reduzir translocação bacteriana em I/R intestinal. Numa escala de dois a 15 minutos, o melhor tempo de pré-condicionamento isquêmico para a atenuação da translocação bacteriana foi 15 minutos.

  15. Influência das estruturas bacterianas externas na inativação fotodinâmica por uma porfirina catiónica

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    Pereira, Marlene António

    2014-01-01

    Os principais alvos da inativação fotodinâmica (PDI) são as estruturas bacterianas externas, membrana citoplasmática e parede celular. Neste trabalho foi avaliado o efeito das estruturas bacterianas externas na eficiência da PDI. Para alcançar este objectivo foram selecionadas 8 bactérias com estruturas externas distintas; 4 bactérias de Gram negativo (Escherichia coli, com estruturas externas típicas das bactérias de Gram negativo; Aeromonas salmonicida, Aeromonas hydrophila ambas com uma ca...

  16. In vitro bacterial plaque suppression and recolonization by S. mutans and S. sobrinus Supressão e recolonização de placa bacteriana por S. mutans e S. sobrinus in vitro

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    Cássio Vicente Pereira

    2006-03-01

    que foram previamente padronizadas foram incubadas em meio de cultura contendo diferentes carboidratos fermentáveis. Em intervalos de tempo determinados, amostras de S. mutans e S. sobrinus foram coletadas a partir de culturas mistas, diluídas e semeadas em placas com meio BHI-ágar contendo rifampicina ou estreptomicina para determinação do número de células viáveis de cada espécie por contagem de unidades formadoras de colônia. Para a avaliação da colonização bacteriana e recolonização da placa bacteriana in vitro, três experimentos foram realizados: I - co-cultivo de S. mutans e S. sobrinus; II - inoculação de S. mutans em placa bacteriana pré-formada por S. sobrinus; e III - placa bacteriana pré-formada por S. mutans dispersada e plaqueada em meio BHI-ágar contendo estreptomicina ou rifampicina para determinação do número de células viáveis para cada espécie. Os resultados indicaram uma predominância de S. mutans em relação ao S. sobrinus, demonstrando a capacidade do S. mutans em inibir a formação de placa por S. sobrinus e recolonizar a superfície dentária.

  17. La necrosis bacteriana de la vid, causada por xylophilus ampelinus. Detección serológica, distribución en Aragón y sensibilidad varietal.

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    CAMBRA ALVAREZ, MIGUEL

    2011-01-01

    La necrosis bacteriana de la vid, causada por la bacteria de cuarentena Xylophilus ampelinus, es una enfermedad de difícil control químico y que ha causado en España importantes pérdidas económicas. Actualmente, es endémica en distintas zonas de Aragón y Galicia y se han identificado focos aislados en La Rioja. Ante la carencia de métodos sensibles y específicos de detección de esta bacteria que se puedan utilizar para el análisis rutinario, se ha puesto a punto un método de ex...

  18. Caracterização da comunidade bacteriana endofítica de citros por isolamento, PCR específico e DGGE Characterization of the endophytic bacterial community from citrus by isolation, specific PCR and DGGE

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    Paulo Teixeira Lacava

    2006-04-01

    Full Text Available O objetivo deste trabalho foi caracterizar a comunidade bacteriana endofítica de plantas assintomáticas (escapes e afetadas pela clorose variegada dos citros (CVC por meio de isolamento em meio de cultura, técnica de gradiente desnaturante em gel de eletroforese (DGGE e detecção de Methylobacterium mesophilicum e Xyllela fastidiosa por meio de PCR específico, para estudar esta comunidade e sua relação com a ocorrência da CVC. A análise da comunidade bacteriana via DGGE permitiu a detecção de X. fastidiosa, bem como Klebsiella sp. e Acinetobacter sp. como endófitos de citros. Foram observados também Curtobacterium sp., Pseudomonas sp., Enterobacter sp. e Bacillus spp. Utilizando primers específicos, Methylobacterium mesophilicum e X. fastidiosa também foram observadas, reforçando hipóteses de que estas bactérias podem estar interagindo no interior da planta hospedeira.The aim of this work was to characterize endophytic bacterial community of assintomatic (escape and Citrus Variegated Chlorosis (CVC-affected citrus plants using isolation in culture medium, denaturing gradient gel electrophoresis (DGGE technique and Methylobacterium mesophilicum as well as Xylella fastidiosa specific PCR, allowing to assess this community and its interactions with CVC. The study of bacterial community by DGGE analysis allowed the detection of X. fastidiosa, as well as Klebsiella sp. e Acinetobacter sp., which were not detected previously. Curtobacterium sp., Pseudomonas sp., Enterobacter sp. and Bacillus spp. were also observed as endophyte in citrus plants. Using specific primers Methylobacterium mesophilicum and X. fastidiosa were observed, reinforcing that these bacteria could interact inside the host plant.

  19. Meningitis bacteriana Bacterial meningitis

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    Ana Teresa Alvarado Guevara

    2006-03-01

    Full Text Available En Costa Rica la meningitis bacteriana se ha convertido en un tema prioritario en lo que a vigilancia epidemiológica se refiere, en los últimos meses se ha dado un aumento en la atención pública de este tema, debido a este fenómeno se hace necesario realizar una revisión del tema. La meningitis es una inflamación de las leptomeninges y colonización del líquido cefalorraquídeo (LCR debido a diferentes agentes, lo cual produce síntomas meníngeos (Ej., cefalea, rigidez nucal, fotofobia y pleocitosis a nivel de LCR. Dependiendo de las variables se pueden agrupar en diferentes clasificaciones, tomando en cuenta el tiempo de evolución se pueden dividir en agudas o crónicas, a las primeras con pocas horas o días de inicio de la sintomatología, mientras que la crónica presenta un curso mas larvado de la enfermedad de aproximadamente 4 semanas de instauración. Existe también diferencia según su etiología, pueden ser infecciosas y no infecciosas. Causas no infecciosas incluyen: drogas antiinflamatorias, antibióticos y carcinomatosis. A su vez existe una clasificación según el agente causal. La meningitis bacteriana aguda remarca el origen bacteriano de este síndrome, el cual se caracteriza por el inicio agudo de sus síntomas y pleocitosis de predominio neutrofílico. Cada uno de los agentes bacterianos, parasíticos o fúngicos terminan por categorizar las diferentes presentaciones de este cuadro clínico (Ej., meningitis meningocóccica, meningitis criptocóccica. Es en este grupo en específico de etiología en el cual se basara el siguiente artículo. Por último pero no menos importante tenemos la meningitis aséptica, denominada de esta forma debido a una respuesta celular no pirógena causada por muchos tipos de agentes. Los pacientes muestran un inicio agudo de síntomas meníngeos, fiebre y pleocitosis pero de predominio linfocítico. Después de análisis especializados, se da pro concluido que la mayoría de los agentes

  20. Estudo dos parâmetros da ultrafiltração de permeado de soro de queijo fermentado por Lactococcus lactis subsp. lactis Ultrafiltration conditions of whey permeate fermented by Lactococcus lactis subsp. lactis

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    Viviane BRONSTEIN

    1998-04-01

    Full Text Available Permeado de soro doce, suplementado com extrato de levedura e peptona, foi utilizado como meio de crescimento para Lactococcus lactis subsp. lactis. No final da fase exponencial de crescimento, o meio de cultura fermentado foi submetido a uma ultrafiltração com o objetivo de concentrar o microrganismo. Foram realizados 6 processamentos diferentes, nos quais variou-se as condições iniciais da ultrafiltração, tendo sido avaliados os seguintes parâmetros: porosidade da membrana, pH e número de células viáveis no permeado e no retentado, a fim de ser estudado a influência de cada parâmetro na taxa de permeação da ultrafiltração. As membranas utilizadas foram eficazes como meio de barragem para o microrganismo Lactococcus lactis subsp. lactis, ficando o retentado com uma média celular de 10(8 ufc/ml e o permeado com uma média celular de 10² ufc/ml. Membranas de diferentes porosidades tiveram taxas de fluxo semelhantes. O aumento da concentração celular provocou a diminuição do fluxo. O pH também influenciou a taxa de permeação, havendo um aumento do fluxo quando foi utilizado um pH inicial mais alto.Cheese whey permeate supplemented with yeast extract and peptone was used as a growth medium for the bacteria Lactococcus lactis subsp. lactis. At the end of the exponential growth phase, the fermented growth medium was ultrafiltered to concentrate the microorganism and to evaluate the effect of the membrane porosity, inicial UF pH and cellular concentration in permeation rate during the ultrafiltration process. The membranes used were efficient as a mean of a barrage for the Lactococcus lactis subsp. lactis. On average, the cellular concentrations were 10(8 CFU/mL and 10² CFU/mL for retentate and permeate, respectively. Membranes of different porosities had very similar flux rates. Better flow rates were obtained with inicial UF pH 6,5 and with the minors micrrorganism concentration.

  1. Meningites bacterianas recidivantes

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    Maria Valeriana L. Moura-Ribeiro

    1992-06-01

    Full Text Available O estudo das fístulas liquóricas, envolvendo anormalidades otorrinolaringológicas, deve se basear na procura da causa fundamental que leva às meningites recidivantes. As malformações congênitas, as anormalidades pós-traumáticas e pós-operatórias ou, ainda, associadas a doenças que envolvem ossos cranianos, constituem as causas básicas a serem pesquisadas. Os traumas cranianos podem resultar em fístula liquórica com possibilidade de meningites bacterianas de repetição.

  2. Meningitis bacteriana Bacterial meningitis

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    Ana Teresa Alvarado Guevara; Lizzie Marie Castillo Solano

    2006-01-01

    En Costa Rica la meningitis bacteriana se ha convertido en un tema prioritario en lo que a vigilancia epidemiológica se refiere, en los últimos meses se ha dado un aumento en la atención pública de este tema, debido a este fenómeno se hace necesario realizar una revisión del tema. La meningitis es una inflamación de las leptomeninges y colonización del líquido cefalorraquídeo (LCR) debido a diferentes agentes, lo cual produce síntomas meníngeos (Ej., cefalea, rigidez nucal, fotofobia) y pleoc...

  3. Biofertilizantes no controle da mancha bacteriana (Xanthomonas spp.) do tomateiro

    OpenAIRE

    Vinícius William Borges Rodrigues; Thays Vieira Bueno; Nilvanira Donizete Tebaldi

    2016-01-01

    RESUMO A mancha bacteriana do tomateiro, causada por quatro espécies de Xanthomonas pode provocar perdas significativas na produção da cultura e a utilização de biofertilizantes na proteção de plantas tende a reduzir a incidência de doenças. O objetivo do trabalho foi avaliar o efeito dos biofertilizantes no controle preventivo e curativo da mancha bacteriana do tomateiro. Para o controle preventivo da doença, plantas de tomate cultivar Santa Cruz Kada, com 3 a 4 folhas foram pulverizadas com...

  4. Producción de ácido láctico por una mezcla de lactococcus lactis y streptococcus salivarius en fermentaciones en discontinuo

    OpenAIRE

    Serna Cock, Liliana; Rodríguez de Stouvenel, Aida

    2006-01-01

    Se estudió la producción de ácido láctico (AL), la conversión de sustrato (CG), y el rendimiento(Yp/s) de Lactococcus lactis, Streptococcus salivarius y una mezcla 1:1 de ambas cepas en sustrato glucosado. Lactococcus lactis se seleccionó de 20 cepas homofermentativas aisladas de cultivos de caña de azúcar variedad CC85-92 y Streptococcus salivarius se aisló de un fermento láctico comercial. En fermentaciones llevadas a cabo con la mezcla microbiana, a 32 °C con 60 gL-1 de glucosa y pH 6,0 se...

  5. Biofertilizantes no controle da mancha bacteriana (Xanthomonas spp. do tomateiro

    Directory of Open Access Journals (Sweden)

    Vinícius William Borges Rodrigues

    2016-03-01

    Full Text Available RESUMO A mancha bacteriana do tomateiro, causada por quatro espécies de Xanthomonas pode provocar perdas significativas na produção da cultura e a utilização de biofertilizantes na proteção de plantas tende a reduzir a incidência de doenças. O objetivo do trabalho foi avaliar o efeito dos biofertilizantes no controle preventivo e curativo da mancha bacteriana do tomateiro. Para o controle preventivo da doença, plantas de tomate cultivar Santa Cruz Kada, com 3 a 4 folhas foram pulverizadas com os biofertilizantes (Soil-Set, Agro-Mos e Cop-R-Quick e água (testemunha; e dois dias após foram inoculadas por aspersão com a suspensão bacteriana nas concentrações 109 UFC mL-1 (OD550=0,5 e 106UFC mL-1, com o isolado UFU A35 de Xanthomonas sp. Para o controle curativo, as plantas foram inoculadas com a suspensão bacteriana, e dois dias após foram pulverizadas com os biofertilizantes e água. A severidade da mancha bacteriana foi avaliada usando uma escala diagramática; aos 3, 5, 8, 11 e 14 dias após a inoculação e calculada a área abaixo da curva de progresso de doença (AACPD. O controle preventivo foi mais eficiente no manejo da mancha bacteriana do tomateiro, e os diferentes biofertilizantes reduziram a severidade da doença.

  6. Otimização da produção de nata (celulose bacteriana) por fermentação em superfície

    OpenAIRE

    DANESI Eliane Dalva Godoy; Wosiacki, Gilvan

    1998-01-01

    A nata de coco, alimento glicídico obtido por fermentação em superfície promovida por Acetobacter xylinum, é bastante difundida em alguns países asiáticos, principalmente nas Filipinas. Como meio de cultivo são utilizadas a água ou o leite de coco, produtos de baixo valor econômico e resíduos de processamento da fruta; há indicativos na literatura, entretanto, de que outros resíduos agro-industriais como soro de leite ou mesmo suco de frutas podem ser utilizados. A fim de avaliar a produção d...

  7. Participação de receptores NK1 nas respostas inflamatória e febril induzidas por endotoxina bacteriana

    OpenAIRE

    Reis, Renata Cristiane dos

    2010-01-01

    Resumo: As respostas inflamatoria e febril sao importantes para a defesa do organismo contra agentes agressores. Mediadores inflamatorios liberados por celulas do sistema imune e neurotransmissores e peptideos provenientes de fibras nervosas, especialmente das fibras vagais, sao importantes na interligacao destas respostas. Estudos anteriores realizados em nosso laboratorio evidenciaram a importancia do nervo vago e da acetilcolina, o principal neurotransmissor das fibras eferentes vagais, na...

  8. Murcha bacteriana no estado do Amazonas, Brasil Bacterial wilt in Amazonas State, Brazil

    OpenAIRE

    ROSALEE A. COELHO NETTO; Bianca G. Pereira; Hiroshi Noda; Bernard Boher

    2004-01-01

    Durante 1998 e 2000, a incidência de murcha bacteriana causada por Ralstonia solanacearum foi registrada em 25 municípios do estado do Amazonas. A bactéria foi encontrada nas seguintes espécies vegetais: Capsicum annuum, C. chinense, C. frutescens, Cucumis sativus, Heliconia sp., Lycopersicon esculentum, Melanthera discoidea, Moringa oleifera, Musa sp., Solanum melongena, S. gilo, e S. nigrum. Em tomateiros (Lycopersicon esculentum), a murcha bacteriana estava presente em todos os plantios. E...

  9. Características da bacteriocina produzida por Lactococcus lactis ssp. hordniae CTC 484 e seu efeito sobre Listeria monocytogenes em carne bovina Characterisation of the bacteriocin produced by Lactococcus lactis ssp. hordniae CTC 484 and the effect of this compound on Listeria monocytogenes in beef

    Directory of Open Access Journals (Sweden)

    Renata Bromberg

    2006-03-01

    Full Text Available O isolamento de linhagens de bactérias lácticas produtoras de bacteriocinas em carnes e seus produtos derivados resultou na detecção de Lactococcus lactis ssp. hordniae CTC 484, proveniente de frango. A bacteriocina inibiu não apenas uma outra bactéria láctica (Lactobacillus helveticus, mas também microorganismos patogênicos (Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus, Clostridium perfringens e Enterococcus faecalis. Ela foi inativada por causa de enzimas como: alfa-quimotripsina, tripsina, pronase E, ficina, pepsina, papaína e lipase. Além disso, a bacteriocina mostrou-se termoestável, mesmo a temperaturas de autoclavagem (121°C/10 min e foi produzida em condições de armazenamento sob refrigeração. A bacteriocina mostrou-se ativa dentro de uma ampla faixa de valores de pH (2-10, porém a maior atividade ocorreu em valores menores de pH. A eficiência da linhagem CTC 484, assim como a de sua bacteriocina na redução e inibição do crescimento de Listeria monocytogenes em carne bovina estéril, foram avaliadas. Os resultados indicaram que o tratamento da carne por meio da inoculação desta bactéria contribuiu para o aumento da segurança e extensão da vida útil deste alimento.Screening for the bacteriocin production of strains of lactic acid bacteria from various meat and meat products resulted in the detection of a bacteriocin-producing Lactococcus lactis ssp. hordniae CTC 484, isolated from chicken. The bacteriocin inhibited not only closely related lactic acid bacterium (Lactobacillus helveticus, but also pathogenic microorganisms (Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus, Clostridium perfringens, and Enterococcus faecalis. This compound was inactivated by alpha-chymotrypsin, trypsin, pronase E, ficin, pepsin, papain, and also by lipase. It was heat stable even at autoclaving temperature (121°C/10 min and was produced under refrigerated storage. It was also active over a wide

  10. Las enfermedades periodontales como infecciones bacterianas

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    A. Bascones Martínez

    2005-12-01

    Full Text Available Las infecciones periodontales son un conjunto de enfermedades localizadas en las encías y estructuras de soporte del diente. Están producidas por ciertas bacterias provenientes de la placa bacteriana. Estas bacterias son esenciales para el inicio de la enfermedad, pero existen factores predisponentes del hospedador y microbianos que influyen en la patogénesis de la enfermedad. La microbiota bacteriana periodontopatógena es necesaria pero no suficiente para que exista enfermedad, siendo necesaria la presencia de un hospedador susceptible. Estas enfermedades se han clasificado en gingivitis, limitadas a las encías y periodontitis, extendidas a tejidos más profundos. La clasificación de las enfermedades periodontales ha ido variando a lo largo de los años y es en el International Workshop for a Clasification of Periodontal Diseases and Conditions, en 1999, cuando se aprueba la clasificación que se expone en este trabajo. En él, se hace una revisión global de los diferentes cuadros de las enfermedades periodontales. Posteriormente, se propone el empleo de antibioterapia de utilización sistémica como la amoxicilina, amoxicilina-clavulánico y metronidazol como primera opción de tratamiento coadyuvante de estas enfermedades.

  11. Quando a traqueíte bacteriana se complica...

    OpenAIRE

    Teixeira, Joana; Pereira, Ângela; Oliveira, Ângela; Moreira, Filipa; Alves, Manuela Costa; Dias, Luís; Gonçalves, Augusta; Moreira, Carla

    2015-01-01

    Introdução: A laryngotracheobronchitis (LTB) vírica constitui a causa mais comum de estridor nas crianças que recorrem ao Serviço de Urgência. Na ausência de resposta ao tratamento com corticóide e adrenalina nebulizada, a traqueíte bacteriana deve ser considerada. Caso Clínico: Criança de seis anos de idade observada no Serviço de Urgência por estridor, sinais de dificuldade respiratória e febre com resposta parcial à terapêutica com adrenalina nebulizada; por suspeita de LTB com sobrein...

  12. Cambios en las comunidades bacterianas de suelo luego de una contaminación con hexadecano

    OpenAIRE

    Pucci, Graciela N.; Adrián J. Acuña; Natalia Y. Nohra; Oscar H. Pucci

    2012-01-01

    El presente trabajo informa sobre los cambios en la comunidad bacteriana de un suelo proveniente de una zona hidrocarburífera de Argentina. Los cambios se observaron en un ensayo de microcosmo el cual fue sometido a contaminación con hexadecano. La determinacion de hidrocarburos y los recuentos bacterianos fueron realizados semanalmente. Los cambios en la diversidad bacteriana se determinaron por la el análisis de los ácidos grasos de membrana (FAMEs); identificándose y cuantificándose por cr...

  13. Cambios en las comunidades bacterianas de suelo luego de una contaminación con hexadecano

    Directory of Open Access Journals (Sweden)

    Graciela N. Pucci

    2012-10-01

    Full Text Available El presente trabajo informa sobre los cambios en la comunidad bacteriana de un suelo proveniente de una zona hidrocarburífera de Argentina. Los cambios se observaron en un ensayo de microcosmo el cual fue sometido a contaminación con hexadecano. La determinacion de hidrocarburos y los recuentos bacterianos fueron realizados semanalmente. Los cambios en la diversidad bacteriana se determinaron por la el análisis de los ácidos grasos de membrana (FAMEs; identificándose y cuantificándose por cromatografía gaseosa utilizando los parámetros según MIDI. Se observó que la contaminación con hexadecano causa un disturbio en el suelo que conduce a un cambio en la estructura de la comunidad bacteriana.

  14. Producción de ácido láctico por una mezcla de Lactococcus lactis y Streptococcus salivarius en fermentaciones en discontinuo Lactic acid production from a mixture of cultures of Lactococcus lactis and Streptococcus salivarius using batch fermentation

    Directory of Open Access Journals (Sweden)

    Rodríguez de Stouvenel Aida

    2005-07-01

    Full Text Available Se estudió la producción de ácido láctico (AL, la conversión de sustrato (CG, y el rendimiento(Yp/s de Lactococcus lactis, Streptococcus salivarius y una mezcla 1:1 de ambas cepas en sustrato glucosado. Lactococcus lactis se seleccionó de 20 cepas homofermentativas aisladas de cultivos de caña de azúcar variedad CC85-92 y Streptococcus salivarius se aisló de un fermento láctico comercial. En fermentaciones llevadas a cabo con la mezcla microbiana, a 32 °C con 60 gL-1 de glucosa y pH 6,0 se obtuvo un máximo de 47,63 gL-1 de ácido láctico, conversión de glucosa de 95,4% y rendimiento en producto de 0,83 gg-1. Palabras clave: caña de azúcar, Lactococcus lactis, Streptococcus salivarius, mezcla de cepas.Production of lactic acid (LA, yield (Yp/s and substrate conversion (SC from Lactococcus lactis, Streptococcus salivarius and their mixtures were tested. Lactococcus lactis was selected from 20 homofermentative strains isolated from a sugar cane crop (variety CC85-92 and Streptococcus salivarius was isolated from a commercial lactic ferment. Batch fermentation experiments at 32 C with a glucose concentration of 60 gL-1 and a pH of 6,0 were carried out. A maximum of 47,63 gL-1 of lactic acid concentration, 95,4% of substrate conversion and 83 gg-1 were obtained from the mixture of strains after a fermentation of 48 h. Key words: sugar cane, Lactococcus lactis, Streptococcus salivarius, mixture of strains.

  15. Antibióticos vs. resistencia bacteriana

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    Elso Manuel Cruz Cruz

    2015-02-01

    Full Text Available La resistencia bacteriana a los antibióticos es un problema de salud mundial que se encuentra en constante evolución. De manera frecuente se reportan nuevos mecanismos de resistencia bacteriana a los antibióticos, tanto en bacterias gram negativas como en gram positivas. La presencia de resistencia en una bacteria causante de infección disminuye las posibilidades de obtener la curación clínica y la erradicación bacteriológica e incrementa los costos del tratamiento, la morbilidad y la mortalidad; por lo que es importante seleccionar el tratamiento adecuado. Uno de los aspectos que más preocupa a los médicos en el diagnóstico y tratamiento de una infección es la llamada resistencia adquirida, la cual ocurre en una bacteria inicialmente sensible a los antibióticos, por cambios, mutaciones o la adquisición de genes de resistencia durante el fenómeno de transferencia genética lateral, proceso por medio del cual un organismo transfiere material genético a otra célula que no es descendiente. La resistencia bacteriana adquirida a los antibióticos puede ser de distintos tipos, dependiendo de la presión selectiva, las mutaciones o la transferencia de genes de resistencia. Las definiciones de resistencia se clasifican según el número y clase de antibióticos afectados. La multirresistencia (Multiple Drug Resistance, MDR se define como la ausencia de sensibilidad a, por lo menos, un fármaco en tres o más de las categorías de antibióticos; la resistencia extrema (Extensively Drug-Resistant, XDR se refiere a la ausencia de sensibilidad a, por lo menos, un agente en todas las categorías de antimicrobianos, excepto en dos de ellas o menos, y la resistencia a todos los antimicrobianos se define como resistencia a todas las categorías de antibióticos. (1 Los mecanismos de resistencia dependen del tipo de bacteria que los desarrollen. Las bacterias gram positivas que producen con más frecuencias infecciones en humanos y que por

  16. Duchas vaginales y otros riesgos de vaginosis bacteriana

    OpenAIRE

    Natividad Chávez; Helfer Molina; Jorge Sánche; Bizu Gelaye; Sánchez, Sixto E

    2009-01-01

    La vaginosis bacteriana (VB) es una infección caracterizada por el cambio en la microflora de la vagina, se asocia con resultados adversos del embarazo y a la adquisición de infecciones de transmisión sexual (ITS), que incluyen el VIH. Objetivos. Identificar la asociación entre el uso de duchas vaginales y otros factores de riesgo con VB. Materiales y métodos. Se realizó un estudio transversal en 1252 mujeres que asistieron al servicio de planificación familiar de tres hospitales nacionales y...

  17. Actividad sialidasa en mujeres con vaginosis bacteriana Sialidase activity in women with bacterial vaginosis

    OpenAIRE

    Adriana M. Ombrella; Adriana Belmonte; Mónica G. Nogueras; Isabel Ruiz Abad; Emma G. Sutich; Dlugovitzky, Diana G.

    2006-01-01

    La vaginosis bacteriana (VB) es un síndrome caracterizado por el sobrecrecimiento bacteriano de flora endógena Gram negativa, que desplaza a la flora lactobacilar normal. Dentro de las enzimas bacterianas, las sialidasas han sido consideradas factores de virulencia de muchos microorganismos patógenos que colonizan las distintas mucosas. Su presencia en fluidos vaginales puede estar correlacionada con VB. El propósito de este estudio fue comprobar la actividad de dicha enzima en mujeres con es...

  18. Infecção bacteriana no paciente cirrótico

    OpenAIRE

    Mattos Angelo A. de; Coral Gabriela P.; Menti Eduardo; Valiatti Fabiana; Kramer Caroline

    2003-01-01

    RACIONAL: Em torno de 30% dos pacientes com cirrose hepática apresentam infecção bacteriana na admissão hospitalar ou a desenvolvem durante a mesma. As infecções bacterianas são responsáveis por até 25% das mortes nesta população de pacientes. OBJETIVO: Avaliar a prevalência de infecção bacteriana em uma população de pacientes cirróticos internados em hospital geral, bem como correlacionar sua presença com a etiologia alcoólica da hepatopatia, com o grau de comprometimento da função hepática ...

  19. Producción de ácido láctico por una mezcla de Lactococcus lactis y Streptococcus salivarius en fermentaciones en discontinuo Lactic acid production from a mixture of cultures of Lactococcus lactis and Streptococcus salivarius using batch fermentation

    OpenAIRE

    Rodríguez de Stouvenel Aida; Serna Cock Liliana

    2005-01-01

    Se estudió la producción de ácido láctico (AL), la conversión de sustrato (CG), y el rendimiento(Yp/s) de Lactococcus lactis, Streptococcus salivarius y una mezcla 1:1 de ambas cepas en sustrato glucosado. Lactococcus lactis se seleccionó de 20 cepas homofermentativas aisladas de cultivos de caña de azúcar variedad CC85-92 y Streptococcus salivarius se aisló de un fermento láctico comercial. En fermentaciones llevadas a cabo con la mezcla microbiana, a 32 °C con 60 gL-1 de glucosa y pH 6,0 se...

  20. Programa preventivo da cárie dentária baseado no controle mecânico da placa bacteriana em crianças, por meio da profilaxia profissional periódica: Resultados após 25 anos de acompanhamento Dental caries prevention program based on the mechanical control of the bacterial plaque in children, through the periodic professional prophylaxis: Results after 25 years of attendance

    OpenAIRE

    José Eduardo de Oliveira Lima

    2009-01-01

    OBJETIVOS: o objetivo deste trabalho foi verificar a incidência de cárie dentária em um programa de prevenção aplicado durante 25 anos em clínica particular. METODOLOGIA: participaram desse programa 640 crianças de ambos os gêneros, na faixa etária de 3 a 15 anos de idade. O programa foi baseado no controle mecânico da placa bacteriana dentária por meio da profilaxia profissional com jato de bicarbonato de sódio com uma periodicidade mensal. A incidência de cárie foi verificada por meio de ex...

  1. Peritonite bacteriana espontânea

    OpenAIRE

    Strauss Edna; Caly Wanda Regina

    2003-01-01

    A peritonite bacteriana espontânea ocorre em 30% dos cirróticos com ascite e, neste grupo, apresenta altas taxas de morbidade e mortalidade. Os fatores predisponentes incluem a diminuição da defesa imunológica encontrada no homem nas fases avançadas da cirrose, o supercrescimento da flora intestinal e a translocação bacteriana da luz dos intestinos aos linfonodos mesentéricos. As manifestações clínicas variam de graves a leves ou ausentes, sendo sempre necessária a análise do líquido ascítico...

  2. Antibióticos vs. resistencia bacteriana

    OpenAIRE

    Elso Manuel Cruz Cruz

    2015-01-01

    La resistencia bacteriana a los antibióticos es un problema de salud mundial que se encuentra en constante evolución. De manera frecuente se reportan nuevos mecanismos de resistencia bacteriana a los antibióticos, tanto en bacterias gram negativas como en gram positivas. La presencia de resistencia en una bacteria causante de infección disminuye las posibilidades de obtener la curación clínica y la erradicación bacteriológica e incrementa los costos del tratamiento, la morbilidad y la mortali...

  3. Peritonite bacteriana espontânea

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    Strauss Edna

    2003-01-01

    Full Text Available A peritonite bacteriana espontânea ocorre em 30% dos cirróticos com ascite e, neste grupo, apresenta altas taxas de morbidade e mortalidade. Os fatores predisponentes incluem a diminuição da defesa imunológica encontrada no homem nas fases avançadas da cirrose, o supercrescimento da flora intestinal e a translocação bacteriana da luz dos intestinos aos linfonodos mesentéricos. As manifestações clínicas variam de graves a leves ou ausentes, sendo sempre necessária a análise do líquido ascítico. O diagnóstico de peritonite bacteriana espontânea se faz pela contagem de neutrófilos > 250/mm³ no líquido ascítico associado ou não ao crescimento de bactéria na cultura. As enterobactérias predominam como causa da infecção, sendo a Echerichia coli a bactéria mais freqüentemente isolada. O diagnóstico precoce e o tratamento adequado provocaram a queda das taxas de mortalidade nas duas últimas décadas. O uso endovenoso de cefalosporinas de terceira geração mostra-se eficaz em 70% a 95% dos casos. A recorrência de peritonite bacteriana espontânea é comum e pode ser prevenida com norfloxacina oral, de uso contínuo. O surgimento de resistência bacteriana tem estimulado a procura de novas opções para a profilaxia da peritonite bacteriana espontânea; os probióticos constituem nova abordagem promissora, mas que necessita melhor avaliação. Recomenda-se a profilaxia primária de curta duração aos cirróticos com ascite que apresentem episódio de hemorragia digestiva alta.

  4. Fontes de silício no controle da mancha bacteriana (Xanthomonas spp. do tomateiro

    Directory of Open Access Journals (Sweden)

    Thaíssa Vitorino dos Anjos

    2014-12-01

    Full Text Available A mancha bacteriana do tomateiro causada por quatro espécies de Xanthomonas pode provocar perdas significativas na produção da cultura e a utilização do silício na proteção de plantas tende a reduzir a incidência de doenças. O objetivo do trabalho foi avaliar fontes de silício no controle da mancha bacteriana do tomateiro. Para a avaliação da inibição do crescimento bacteriano in vitro foram utilizados discos de papel de filtro esterilizados contendo 10 µL de silício coloidal ou silicato de potássio nas concentrações de 10, 30, 40 e 50 µg µL-1. Esses discos foram colocados sobre a bactéria cultivada em placas de Petri com meio de cultura, observando-se a formação de halos de inibição. Para avaliação da redução da severidade da mancha bacteriana do tomateiro em casa de vegetação, plantas de tomate foram pulverizadas com os produtos nas concentrações 10, 20, 30, 40 e 50 g L-1 e, após três dias, foi feita a inoculação por aspersão da suspensão bacteriana (109 UFC mL-1. Como testemunhas foram utilizadas plantas pulverizadas com água destilada ou inoculadas com a suspensão bacteriana. O silício coloidal não foi eficiente no controle de Xanthomonas spp. Concentrações de 30, 40 e 50 µg µL-1 de silicato de potássio inibiram o crescimento bacteriano in vitro e concentrações de 40 e 50 g L-1 reduziram o índice de doença da mancha bacteriana do tomateiro.

  5. Fontes de silício no controle da mancha bacteriana (Xanthomonas spp.) do tomateiro

    OpenAIRE

    Thaíssa Vitorino dos Anjos; Nilvanira Donizete Tebaldi; Lara Caroline Borges Moreira Mota; Lísias Coelho

    2014-01-01

    A mancha bacteriana do tomateiro causada por quatro espécies de Xanthomonas pode provocar perdas significativas na produção da cultura e a utilização do silício na proteção de plantas tende a reduzir a incidência de doenças. O objetivo do trabalho foi avaliar fontes de silício no controle da mancha bacteriana do tomateiro. Para a avaliação da inibição do crescimento bacteriano in vitro foram utilizados discos de papel de filtro esterilizados contendo 10 µL de silício coloidal ou silicato de p...

  6. Bacteriocinas de Lactococcus lactis aislados de quesos asturianos: Nisina Z y Lactococina 972

    OpenAIRE

    Martínez Fernández, Beatriz

    1996-01-01

    Se ha analizado la producción de bacteriocinas en cepas de lactococcus aisladas de quesos artesanales asturianos, de este modo se han identificado productores de Nisina Z que producen la bacteriocina en leche y, además, presentan aptitudes tecnológicas adecuadas para su inclusión en un cultivo iniciador. Por otro lado, se ha descrito una nueva bacteriocina Lactococina 972, cuyo espectro de actuación se restringe al género lactococcus. Esta bacteriocina se ha purificado y se ha obtenido su ...

  7. Microbiota bacteriana da conjuntiva no pré-operatório de injeção intravítrea de antiangiogênico por degeneração macular relacionada à idade comparada com a de cirurgia de catarata Preoperative conjunctival bacterial microbiota of antiangiogenic intravitreous injection for age-related macular degeneration compared to cataract surgery preoperative microbiota

    Directory of Open Access Journals (Sweden)

    José Ricardo Diniz

    2010-06-01

    Full Text Available Objetivo: Avaliar a microbiota bacteriana da conjuntiva e perfil de antibiograma no pré-operatório de injeção intravítrea de antiangiogênico por degeneração macular relacionada à idade, comparando com a de pacientes no pré-operatório de cirurgia de catarata. Métodos: Realizou-se estudo transversal, observacional, tipo série de casos. Foram constituídos dois grupos: grupo I (degeneração macular com 26 olhos de 26 pacientes (12 homens/14 mulheres com média de idades de 69,2 ± 11,5 anos; grupo II (catarata com 27 olhos de 27 pacientes (9 homens/18 mulheres com média de idades de 67,6 ± 7,9 anos. Os grupos foram homogêneos em relação à idade (p=0,538 e ao sexo (p=0,787. Foi realizada coleta de secreção do fundo de saco inferior da conjuntiva, através de "swab", e imediatamente colocado em tubo contendo meio líquido BHI ("brain heart infusion". As amostras foram processadas conforme técnicas laboratoriais padrão e realizado antibiograma de cada colônia isolada. Resultados: Houve crescimento de 26 colônias bacterianas no grupo I, com 2 olhos não apresentando crescimento e 30 colônias no grupo II. Houve maior frequência de bactérias Gram positivas nos dois grupos: 23/26 colônias (88,4% no grupo I e 29/30 colônias (96,7% no grupo II, com predomínio de Staphylococcus aureus em ambos os grupos, com 16 amostras (61,5% e 17 (56,7%, respectivamente. Staphylococcus coagulase negativa foi a segunda bactéria mais identificada, com 19,2% no grupo I e 20,0% no grupo II. Nenhuma diferença de frequência entre os grupos alcançou significância estatística. Não foi observada diferença estatisticamente significante nas sensibilidades das bactérias aos antibióticos testados entre os dois grupos. Conclusões: Não houve diferença na distribuição das bactérias e no perfil de antibiograma da microbiota conjuntival de pacientes no pré-operatório de injeção intravítrea por degeneração macular, comparada a de

  8. Lactococcus lactis - a diploid bacterium

    DEFF Research Database (Denmark)

    Michelsen, Ole; Hansen, Flemming G.; Jensen, Peter Ruhdal

    In contrast to higher eukaryotes, bacteria are haploid, i.e. they store their genetic information in a single chromosome, which is then duplicated during the cell cycle. If the growth rate is sufficiently low, the bacterium is born with only a single copy of the chromosome, which gets duplicated...... before the bacterium divides. Fast-growing bacteria have overlapping rounds of replication, and can contain DNA corresponding to more than four genome equivalents. However, the terminus region of the chromosome is still present in just one copy after division, and is not duplicated until right before...... the next division. Thus, the regions of the chromosome that are the last to be replicated are haploid even in fast-growing bacteria. In contrast to this general rule for bacteria, we found that Lactococcus lactis, a bacterium which has been exploited for thousands of years for the production of fermented...

  9. Infecção bacteriana no paciente cirrótico

    Directory of Open Access Journals (Sweden)

    Mattos Angelo A. de

    2003-01-01

    Full Text Available RACIONAL: Em torno de 30% dos pacientes com cirrose hepática apresentam infecção bacteriana na admissão hospitalar ou a desenvolvem durante a mesma. As infecções bacterianas são responsáveis por até 25% das mortes nesta população de pacientes. OBJETIVO: Avaliar a prevalência de infecção bacteriana em uma população de pacientes cirróticos internados em hospital geral, bem como correlacionar sua presença com a etiologia alcoólica da hepatopatia, com o grau de comprometimento da função hepática e com a presença de hemorragia digestiva alta. PACIENTES E MÉTODOS: Foram avaliadas retrospectivamente 541 internações hospitalares consecutivas em 426 pacientes com cirrose hepática, internados no período de 1992 a 2000. A média de idade destes pacientes foi de 50,5 anos (15-95, sendo 71,2% do sexo masculino. Etiologia alcoólica da hepatopatia esteve presente em 35,4% dos pacientes. Alta ou óbito do paciente no período da internação hospitalar foi considerado como principal desfecho. O nível de significância considerado na análise estatística foi de 5%. RESULTADOS: As infecções bacterianas ocorreram em 25% dos cirróticos (135 episódios. Destas, as mais freqüentes foram: a infecção do trato urinário em 31,1%, a peritonite bacteriana espontânea em 25,9% e a broncopneumonia em 25,2%. Ocorreu associação de infecção urinária e broncopneumonia em 3,7% e infecção de pele e tecido celular subcutâneo em 11,11%. Bacteremia sem foco definido ocorreu nos quatro casos restantes (2,9%. Houve associação da presença de infecção com a etiologia alcoólica da hepatopatia, com a classificação de Child-Pugh e com a ocorrência de hemorragia digestiva alta. A mortalidade hospitalar foi maior nos pacientes infectados (8,9%, estando a mesma associada ao grau de disfunção hepatocelular. CONCLUSÕES: A ocorrência de infecção bacteriana no paciente cirrótico hospitalizado é freqüente e correlaciona-se com a

  10. Adhesión bacteriana a biomateriales Bacterial adhesion to biomaterials

    Directory of Open Access Journals (Sweden)

    C Ábalos

    2005-02-01

    Full Text Available En términos generales, para la adhesión bacteriana, influyen cuatro elementos: Material, Microorganismos, antimicrobianos y mecanismos de defensa. La influencia del material es más importante en los estadios iniciales de la adhesión, pudiendo influir el mismo material, su rugosidad o su energía superficial., si es que existe una influencia del material en la adhesión bacteriana, esta reside en las caracteristicas de la película adquirida y en la especificidad de las proteinas adsorbidas salivares (receptores, que puedan ser condicionadas por la composición del material o por las características de superficie de este.In general terms, there are four elements which influence on bacterial adhesion: the material, the micro organisms, antimicrobials and defence mechanisms. The influence of the material is more relevant at the initial states of adhesion where the proper material, its roughness or its superficial energy can have some influence. If there is some influence of the material in the bacterial adhesion, it relies on the features of the acquired film and on the specificity of the adsorved salivary proteins (receptors, which can be influenced by the composition of the material or the characteristics of its surface.

  11. Las enfermedades periodontales como infecciones bacterianas Periodontal diseases as bacterial infection

    Directory of Open Access Journals (Sweden)

    A. Bascones Martínez

    2005-12-01

    Full Text Available Las infecciones periodontales son un conjunto de enfermedades localizadas en las encías y estructuras de soporte del diente. Están producidas por ciertas bacterias provenientes de la placa bacteriana. Estas bacterias son esenciales para el inicio de la enfermedad, pero existen factores predisponentes del hospedador y microbianos que influyen en la patogénesis de la enfermedad. La microbiota bacteriana periodontopatógena es necesaria pero no suficiente para que exista enfermedad, siendo necesaria la presencia de un hospedador susceptible. Estas enfermedades se han clasificado en gingivitis, limitadas a las encías y periodontitis, extendidas a tejidos más profundos. La clasificación de las enfermedades periodontales ha ido variando a lo largo de los años y es en el International Workshop for a Clasification of Periodontal Diseases and Conditions, en 1999, cuando se aprueba la clasificación que se expone en este trabajo. En él, se hace una revisión global de los diferentes cuadros de las enfermedades periodontales. Posteriormente, se propone el empleo de antibioterapia de utilización sistémica como la amoxicilina, amoxicilina-clavulánico y metronidazol como primera opción de tratamiento coadyuvante de estas enfermedades.

  12. VULVOVAGINITIS BACTERIANA EN LA PREPÚBER

    Directory of Open Access Journals (Sweden)

    Gabriel Barbosa R.

    2005-01-01

    Full Text Available Considerando la alta incidencia de vulvovaginitis en la consulta de ginecología pediátrica y de adolescentes, y los aspectos únicos de la microbiología y endocrinología de la paciente prepúber se presenta una revisión de la literatura con lo más relevante de los últimos 5 años, en lo que se refiere a epidemiología, etiología, diagnóstico y tratamiento de la vulvovaginitis bacteriana específica de la niña prepúber. No existe claridad acerca de la microflora vaginal normal, lo cual dificulta en gran medida la interpretación de muestras. De acuerdo con la evidencia actual podemos decir que ante la vulvovaginitis específica de la prepúber debemos tener en consideración como principales agentes causales: patógenos respiratorios, epidérmicos y entéricos; higiene defectuosa, cuerpos extraños, irritantes, enfermedades dermatológicas vulvares, defectos anatómicos y abuso sexual. Streptococcus hemolitico del grupo A y Haemophilus influenzae son patógenos prevalentesHaving into account the high frequency of vulvovaginitis in the pediatric and adolescent gynecology setting and the unique aspects of the microbiology and endocrinology of the pre pubertal patient, we present a review of the most relevant aspects in the last 5 years, concerning epidemiology, etiology, diagnosis and treatment of specific bacterial vulvovaginitis in the pre pubertal girl. There's no consensus regarding the normal vaginal microflora, which makes difficult the interpretation of swabs. According to actual evidence we could say that in approaching prepubertal vulvovaginitis we must consider as main etiologic agents: respiratory, enteric and epidermic pathogens; deficient hygiene, foreign bodies, irritants, vulvar dermatologic diseases, anatomical abnormalities and sexual abuse. Group A - hemolytic Streptococcus and Haemophilus influenzae are prevalent pathogens

  13. Glutathione protects Lactococcus lactis against oxidative stress

    NARCIS (Netherlands)

    Li, Y.; Hugenholtz, J.; Abee, T.; Molenaar, D.

    2003-01-01

    Glutathione was found in several dairy Lactococcus lactis strains grown in M17 medium. None of these strains was able to synthesize glutathione. In chemically defined medium, L. lactis subsp. cremoris strain SK11 was able to accumulate up to similar to60 mM glutathione when this compound was added t

  14. Dynamics of pyruvate metabolism in Lactococcus lactis

    DEFF Research Database (Denmark)

    Melchiorsen, Claus Rix; Jensen, Niels B.S.; Christensen, Bjarke;

    2001-01-01

    The pyruvate metabolism in the lactic acid bacterium Lactococcus lactis was studied in anaerobic cultures under transient conditions. During growth of L. lactis in continuous culture at high dilution rate, homolactic product formation was observed, i.e., lactate was produced as the major end prod...

  15. Functionality of Sortase A in Lactococcus lactis

    NARCIS (Netherlands)

    Dieye, Yakhya; Oxaran, Virginie; Ledue-Clier, Florence; Alkhalaf, Walid; Buist, Girbe; Juillard, Vincent; Lee, Chang Won; Piard, Jean-Christophe

    2010-01-01

    Lactococcus lactis IL1403 harbors a putative sortase A (SrtA) and 11 putative sortase substrates that carry the canonical LPXTG signature of such substrates. We report here on the functionality of SrtA to anchor five LPXTG substrates to the cell wall, thus suggesting that SrtA is the housekeeping so

  16. Programa preventivo da cárie dentária baseado no controle mecânico da placa bacteriana em crianças, por meio da profilaxia profissional periódica: Resultados após 25 anos de acompanhamento Dental caries prevention program based on the mechanical control of the bacterial plaque in children, through the periodic professional prophylaxis: Results after 25 years of attendance

    Directory of Open Access Journals (Sweden)

    José Eduardo de Oliveira Lima

    2009-06-01

    Full Text Available OBJETIVOS: o objetivo deste trabalho foi verificar a incidência de cárie dentária em um programa de prevenção aplicado durante 25 anos em clínica particular. METODOLOGIA: participaram desse programa 640 crianças de ambos os gêneros, na faixa etária de 3 a 15 anos de idade. O programa foi baseado no controle mecânico da placa bacteriana dentária por meio da profilaxia profissional com jato de bicarbonato de sódio com uma periodicidade mensal. A incidência de cárie foi verificada por meio de exames clínicos durante as sessões de atendimento e radiográficos anualmente. A média de idade das crianças ao ingressarem no programa foi de 7,8 anos. RESULTADOS: antes de ingressar no programa, as crianças apresentaram, em média, 5,3 faces cariadas, enquanto após o programa a média foi de 0,18. A incidência de cáries por ano antes do programa foi de 0,9 faces cariadas e durante o programa de 0,03. O tempo de permanência das crianças no programa foi, em média, de 52,6 meses, e a média de faltas foi de 0,5 falta por ano. Esses resultados demonstram o sucesso do programa, principalmente por ter uma filosofia que procura o equilíbrio da biodiversidade da cavidade bucal, sem o risco de produzir efeitos colaterais indesejáveis. CONCLUSÕES: conclui-se, assim, que esse parece ser o caminho mais curto para resolver o problema da cárie dentária, ou seja, o controle mecânico da placa bacteriana dentária através da profilaxia profissional mensal, pois é um método de prevenção possível de ser aplicado em qualquer criança, independentemente de suas condições psicomotoras e sociais, e que proporciona a melhor relação custo-benefício, além de estar de acordo com os conceitos mais atuais de cárie e de seus fatores etiológicos. Em função da faixa etária das crianças, o programa é de suma importância para clínicas de Odontopediatria e Ortodontia.AIM: This study investigated the incidence of dental caries in a prevention

  17. Infecções bacterianas pioram o prognóstico da hepatite alcoólica Alcoholic hepatitis: bad prognosis due to concomitant bacterial infections

    OpenAIRE

    Edna Strauss; José Pedro Aerosa

    2004-01-01

    As infecções bacterianas cursam com altos índices de morbilidade e mortalidade na cirrose hepática. O objetivo do nosso trabalho foi avaliar se também na hepatite alcoólica as infecções bacterianas são fatores de mau prognóstico. Na avaliação retrospectiva de 681 pacientes hospitalizados em um único centro, por período de 6 anos, foram bem documentados 52 (7,5%) casos de hepatite alcoólica, sendo 73,1% com biópsia hepática para análise histopatológica e os restantes por diagnóstico clínico-bi...

  18. Caracterización epidemiológica y microbiológica de las meningoencefalitis bacterianas

    Directory of Open Access Journals (Sweden)

    Alexis Sanchén Casas

    2010-01-01

    Full Text Available Fundamento: las meningoencefalitis bacterianas constituyen un problema de salud en el planeta representando un reto para los médicos por el manejo del paciente para garantizarle la vida. Se ejecutó el presente trabajo en el Centro Provincial de Higiene, Epidemiologia y Microbiología de Camagüey. Objetivo: mostrar el comportamiento epidemiológico y microbiológico de la meningoencefalitis en la provincia. Método: se realizó un estudio descriptivo y analítico para determinar aspectos acerca del comportamiento epidemiológico y microbiológico de las MEB en la provincia de Camagüey desde el año 2000 hasta el 2008. Constituyeron la muestra ciento noventa y seis casos. Resultados: se demostró un descenso de la morbimortalidad con elevación de la letalidad. Se aislaron ciento ocho cepas. Se diagnosticaron microorganismos como: Streptococcus pneumoniae, Neisseria meningitidis y Haemophilus influenzae tipo b. En infantes se aislaron Salmonella B, Klebsiella pneumoniae, Veillonella y Streptococcus agalactiae. En los adultos hubo una "explosión bacteriana" representada por enterobacterias, bacilos gramnegativos no fermentadores, Staphylococcus aureus y Micrococcus. Encontramos cuarenta cepas causales de fallecimientos: en niños a expensas de Streptococcus pneumoniae, Neisseria meningitidis y Haemophilus influenzae, y, en adultos, Streptococcus pneumoniae y el 100% de enterobacterias, bacilos no fermentadores y Staphylococcus aureus. Conclusiones: el estudio bacteriológico por examen directo y cultivo del LCR constituyó la "prueba de oro" para el diagnóstico de las meningoencefalitis bacterianas.

  19. Programa "Píldoras para la contención de la resistencia bacteriana en una institución hospitalaria"

    OpenAIRE

    Meijueiro Orozco, Martha Lucía

    2011-01-01

    La resistencia bacteriana es un fenómeno sanitario y económico emergente, de origen multifactorial. Para su control es necesario contar con un programa de uso prudente de antibióticos que pueda orientar al prescriptor acerca de los antimicrobianos apropiados para su necesidad y que pueda ser soportado por la evidencia del laboratorio respecto a la susceptibilidad de estos agentes. Igualmente se hace necesario actuar frente al control de las infecciones asociadas al cuidado d...

  20. Validación del método LAL para determinar endotoxinas bacterianas en el inyectable heparina sódica

    Directory of Open Access Journals (Sweden)

    Nancy Burguet

    2012-12-01

    Full Text Available Dentro del control de la calidad de los productos farmacéuticos, la United State Pharmacopeia establece la cuantificación de endotoxinas bacterianas por el método de lisado de amebocito de Limulus , como monitor de pirógenos para más del 90% de los parenterales que regula. Este método se aplicó de forma específica a vacunas bacterianas y virales, agentes antineoplásicos, radiofármacos y parenterales que se producen en la industria médico farmacéutica. En el presente trabajo se mostró la metodología a seguir para realizar la validación de la técnica de determinación de endotoxinas bacterianas por el método de gelificación. Para ello se confirmó la sensibilidad del reactivo utilizado (0,03125 UE/mL y la validez de los analistas para poder obtener resultados confiables. Las pruebas preliminares para el producto ensayado, heparina sódica 5000 UI/mL, demostraron que este producto no potencia ni inhibe la reacción del reactivo. Se escogió la dilución de trabajo (1/128 para la validación del método. De esta manera quedaron estandarizadas las condiciones para la validación del test de lisado de amebocito de Limulus por gelificación en este producto parenteral, método que se hace extensivo a la determinación de endotoxinas bacterianas en vacunas y en otros medicamentos por vía de administración intravenosa.

  1. INTERACCIONES ENTRE Gluconacetobacter diazotrophicus Y LA COMUNIDAD BACTERIANA ENDÓFITA DE LA CAÑA DE AZÚCAR

    OpenAIRE

    Marcia M. Rojas; Odette González; Nidia Rojas; Mayra Heydrich

    2007-01-01

    Gluconacetobacter diazotrophicus es una bacteria endófita de caña de azúcar, que se ha comprobado que es capaz de fijar cantidades considerables de nitrógeno y producir sustancias estimuladoras del crecimiento vegetal, lo cual puede ser de suma importancia en el mejoramiento de este cultivo sobre bases agroecológicas. En el presente trabajo se demuestran, por primera vez, las interacciones que esta especie establece con otros representantes de la comunidad bacteriana endófita de la caña de az...

  2. Displasia de Mondini asociada a meningitis bacteriana recurrente, correlación clínico-imagenológica

    OpenAIRE

    Julianis Loraine Quintero Noa; María del Carmen Hernández Cordero; Ileana Álvarez Lam; Aiza Martínez Romero; Antonio Paz Cordovés; Daisy Hevia Bernal

    2014-01-01

    Se describe la displasia de Mondini asociada a pérdida auditiva y meningitis bacteriana recurrente. La malformación de Mondini representa el 30 % de las anomalías congénitas del oído interno, puede ser unilateral o bilateral, y su principal característica consiste en el desarrollo coclear incompleto, causante de grados variables de hipoacusia neurosensorial. Se considera que la ocurrencia de esta malformación se produce por disrupción del desarrollo embrionario durante la séptima semana de ge...

  3. Efectos de los antioxidantes y la fagocitosis bacteriana sobre la apoptosis de los neutrófilos

    OpenAIRE

    Bonilla del Río, Zuriñe

    2013-01-01

    La fagocitosis bacteriana por los neutrófilos retrasa la apoptosis de los mismos cuando la relación bacteria/neutrófilo es baja, y la acelera cuando dicha relación es alta. Las bacterias también inducen en los neutrófilos la producción de sustancias reactivas de oxígeno que incrementarían su apoptosis, y de citoquinas que podrían retrasarla. El antioxidante DPI (difenileniodonio), inhibidor específico de la NADPH oxidasa de los neutrófilos, podría modificar la apoptosis de...

  4. Estudios estructurales de relaxasas involucradas en el proceso de conjugación bacteriana

    OpenAIRE

    Russi, Silvia

    2010-01-01

    La conjugación bacteriana es uno de los mecanismos de transferencia horizontal genética más importante y la causa principal de la rápida adquisición de resistencia a los antibióticos en las bacterias. La capacidad de las bacterias para conjugarse fue descubierta en 1946 en cultivos de E. coli y, años más tarde, en bacterias Gram-positivas. El proceso de conjugación comienza con el corte de un enlace fosfo-diester específico por medio de una relaxasa que juega un papel clave en la iniciación d...

  5. Biossíntese de celulose bacteriana a partir de resíduos industriais

    OpenAIRE

    Gomes, Fábio Pedro Almeida

    2011-01-01

    A celulose bacteriana (CB) é um biopolímero que devido às suas propriedades estruturais e mecânicas tem uma vasta gama de aplicações em diferentes áreas. Este biopolímero pode ser produzido por algumas bactérias, nomeadamente as pertencentes ao género Gluconacetobacter. No entanto, devido ao custo do meio de cultura a produção de CB a nível industrial tem pouca viabilidade económica. O trabalho realizado teve como objectivo a utilização de diferentes resíduos da Industria Agro-Alimentar para ...

  6. Duchas vaginales y otros riesgos de vaginosis bacteriana

    Directory of Open Access Journals (Sweden)

    Natividad Chávez

    2009-07-01

    Full Text Available La vaginosis bacteriana (VB es una infección caracterizada por el cambio en la microflora de la vagina, se asocia con resultados adversos del embarazo y a la adquisición de infecciones de transmisión sexual (ITS, que incluyen el VIH. Objetivos. Identificar la asociación entre el uso de duchas vaginales y otros factores de riesgo con VB. Materiales y métodos. Se realizó un estudio transversal en 1252 mujeres que asistieron al servicio de planificación familiar de tres hospitales nacionales y un instituto especializado de Lima, durante el año 1997. Se utilizó un cuestionario estructurado donde se registró las variables sociodemográficas y las características del estilo de vida de las participantes. La VB fue diagnosticada mediante el puntaje de Nugent. Se empleó análisis de regresión logística para calcular odds ratio (OR e intervalos de confianza al 95%. Resultados. La edad promedio fue 25,1 ± 4,7 años, el 23,4% tenían más de 11 años de educación. La prevalencia de VB fue 20,1%. Las mujeres que practicaban duchas vaginales (OR: 2,28; IC95%: 1,0- 5,0, que tenían dos o más parejas sexuales (OR: 2,0; IC95%: 1,2-3,5 y que iniciaron una relación sexual a una edad temprana (OR: 1,4; IC95%: 1,0 -1,9 tuvieron mayor probabilidad de adquirir VB. Conclusiones. El uso de duchas vaginales es un factor de riesgo de VB en mujeres peruanas. Los programas destinados a la salud de la mujer deben abordar las repercusiones perjudiciales para la salud asociados con las duchas vaginales.

  7. DUCHAS VAGINALES Y OTROS RIESGOS DE VAGINOSIS BACTERIANA

    Science.gov (United States)

    Chávez, Natividad; Molina, Helfer; Sánchez, Jorge; Gelaye, Bizu; Sánchez, Sixto E.

    2010-01-01

    Vaginosis bacteriana (VB) es una infección caracterizada por el cambio en la microflora de la vagina, asociándose a resultados adversos del embarazo y a la adquisición de infecciones de transmisión sexual (ITS), incluyendo el VIH. En este estudio se buscó la asociación entre el uso de duchas vaginales y otros factores de riesgos con VB. Se usó un diseño observacional descriptivo transversal prospectivo, en 1,252 mujeres que asistieron al servicio de planificación familiar de tres hospitales nacionales (Dos de Mayo, Arzobispo Loayza, San Bartolomé) y el Instituto Materno Perinatal, durante el año 1997. Se utilizó un cuestionario estructurado donde se registraron variables socio demográficas y características del estilo de vida de las participantes. VB fue diagnosticada mediante el puntaje de Nugent. Se empleó análisis de regresión logística para calcular odds ratio (OR) e intervalos de confianza al 95%. La edad promedio de las participantes fue 25.1 ± 4,7 años, el 23.4% tenían más de 11 años de educación. La prevalencía de VB fue 20,1%. Las mujeres que practicaban duchas vaginales tuvieron 2.28 veces (OR = 2.28, IC 95% [1.0–5.0]) mayor probabilidad de tener VB comparado con aquellas que no lo practicaban. Tener dos o más parejas sexuales estuvo asociado con 2.0 veces (OR =2.0, IC 95% [1.2–3.5]) mayor probabilidad de adquirir VB comparado con aquellas que habían tenido solo una pareja sexual. Las participantes que iniciaron una relación sexual a una edad temprana tuvieron 1.4 veces (OR=1.4, IC 95% [1.0 –1.9]) mayor probabilidad de adquirir VB. El uso de duchas vaginales es un factor de riesgo de VB. Los programas destinados a la salud de la mujer deben abordar las repercusiones perjudiciales para la salud asociados con las duchas vaginales. PMID:21132048

  8. Glutathione protects Lactococcus lactis against oxidative stress

    OpenAIRE

    Li, Y.; Hugenholtz, J.; Abee, T.; Molenaar, D

    2003-01-01

    Glutathione was found in several dairy Lactococcus lactis strains grown in M17 medium. None of these strains was able to synthesize glutathione. In chemically defined medium, L. lactis subsp. cremoris strain SK11 was able to accumulate up to similar to60 mM glutathione when this compound was added to the medium. Stationary-phase cells of strain SK11 grown in chemically defined medium supplemented with glutathione showed significantly increased resistance (up to fivefold increased resistance) ...

  9. Glutathione Protects Lactococcus lactis against Oxidative Stress

    OpenAIRE

    2003-01-01

    Glutathione was found in several dairy Lactococcus lactis strains grown in M17 medium. None of these strains was able to synthesize glutathione. In chemically defined medium, L. lactis subsp. cremoris strain SK11 was able to accumulate up to ∼60 mM glutathione when this compound was added to the medium. Stationary-phase cells of strain SK11 grown in chemically defined medium supplemented with glutathione showed significantly increased resistance (up to fivefold increased resistance) to treatm...

  10. Gene inactivation in Lactococcus lactis: histidine biosynthesis.

    OpenAIRE

    Delorme, C; Godon, J J; Ehrlich, S D; Renault, P

    1993-01-01

    Lactococcus lactis strains from dairy and nondairy sources were tested for the ability to grow in the absence of histidine. Among 60 dairy strains tested, 56 required histidine, whereas only 1 of 11 nondairy strains had this requirement. Moreover, 10 of the 56 auxotrophic strains were able to grow in the presence of histidinol (Hol+), the immediate histidine precursor. This indicates that adaptation to milk often results in histidine auxotrophy. The histidine operon was detected by Southern h...

  11. Permeabilidad hidráulica e hinchamiento de membranas de celulosa bacteriana

    Directory of Open Access Journals (Sweden)

    L.A. Caicedo

    2006-01-01

    Full Text Available El grado de hinchamiento y la permeabilidad hidráulica fueron evaluados en membranas de celulosa bacteriana obtenida por cultivo estático de Acetobacter sp. por tres y nueve días, en medio con glucosa y extracto de levadura. Las pruebas de hinchamiento fueron realizadas en disoluciones etanólicas de 0, 10, 50 y 95 % (v/v de etanol, por 24 h a (18 ± 1 °C . El grado de hinchamiento definido como la relación entre la diferencia de peso final e inicial y peso inicial de la membrana, mostró que estas pueden retener 690 veces su peso en agua, en tanto que en disoluciones del 95 % solo se retiene 10 veces su peso seco. Membranas prehinchadas en agua retienen cerca de 4 veces más con respecto a las no prehinchadas. El flujo de permeado es proporcional a la presión aplicada y la permeabilidad hidráulica para membranas de nueve días fue de 0,227 3 mL/kPa199 · cm2 · min, cerca del 50 % de la permeabilidad obtenida en membranas de 3 d . Con disoluciones de etanol las membranas presentaron permeabilidades menores en relación con las obtenidas con agua.

  12. Caracterización de tres cepas bacterianas capaces de fijar nitrógeno y biodegradar hidrocarburos aisladas de un suelo de la Patagonia

    OpenAIRE

    A.J. Acuña; Pucci, G. N.; O.H. Pucci

    2010-01-01

    El objetivo de este trabajo fue caracterizar tres microorganismos capaces de biodegradar hidrocarburos en ausencia de nitrógeno aislados a partir de un suelo de la Patagonia con una larga historia de contaminación por petróleo y sus derivados. Para ello se aislaron tres cepas bacterianas de un suelo patagónico con baja concentración de nitrógeno y contaminado con hidrocarburos. Estos fueron identificados por ARNr 16S y ácidos grasos de membrana. También se estudiaron sus perfiles de biodegrad...

  13. Una nueva ultraestructura de ópalo CT en silcretas. Posible indicador de influencia bacteriana

    Directory of Open Access Journals (Sweden)

    Bustillo, Mª A.

    1995-04-01

    Full Text Available Electron microscope observations of opaline silcretes found in the playa of a Miocene shallow lake reveal new opal CT microstructures. These microstructures are made up of fibres, filaments or rods, which are formed, in tum by coalesced microspheres or microhemispheres (0,1-0,2 µ diameter. They are up to 10 µ in length although larger ones could exist. They are straight, bent, wavy or even hairpin shaped. These microstructures comprise part of the opaline mass of the silcretes and are very similar to both neogenic opaline structures which are formed by bacterial intluence and to silicified biofilms. Therefore microbial mediation could exist in the genesis of this opal CT, although a possible inorganic origin by silicification of sepiolite is also considered.Un estudio mediante MEB de silcretas miocenas opalinas formadas por silicificación de playas en un ambiente de «playa-lake» pone de manifiesto la existencia de una nueva microestructura de ópalo CT. Esta microestructura denominada en general fibrosa, se manifiesta por la presencia de multitud de fibras, filamentos o varillas formadas por la alineación de microesferas o microhemisferas que presentan un diámetro entre 0,1 y 0,2 µ. Las fibras, filamentos o varillas tienen formas variables: rectas, curvadas, onduladas y «en horquilla», y presentan longitudes de hasta 10 µ, aunque podrían existir longitudes mayores. La disposición organizada de las microesferas en filamentos o fibras, constituyendo la masa general del ópalo, y su parecido con microestructuras opalinas que han sido formadas por influencia bacteriana, hacen considerar esta hipótesis, aunque no se puede descartar totalmente un origen inorgánico por silicificación de sepiolita.

  14. CHARACTERIZATION OF LACTOCOCCUS STRAINS AND THEIR USING IN DAIRY TECHNOLOGY

    Directory of Open Access Journals (Sweden)

    Gabriel Greif

    2012-02-01

    Full Text Available Lactococcus lactis species is one of the most important groups of lactic acid bacteria that are used in the dairy industry. Lactococci are generally found on plants and the skins of animals. Special interest is placed on the study of Lactococcus lactis ssp. lactis and Lactococcus lactis ssp. cremoris, as they are the strains used as starter cultures in industrial dairy fermentation. The major functions of this species in dairy fermentation are the production of lactic acid, formation of flavour and aroma compounds, development of ripened cheese texture and antimicrobial activity against spoilage bacteria and moulds.doi:10.5219/162

  15. Rewiring Lactococcus lactis for Ethanol Production

    DEFF Research Database (Denmark)

    Solem, Christian; Dehli, Tore Ibsen; Jensen, Peter Ruhdal

    2013-01-01

    to redirect the metabolism of LAB model organism Lactococcus lactis toward ethanol production. Codon-optimized Zymomonas mobilis pyruvate decarboxylase (PDC) was introduced and expressed from synthetic promoters in different strain backgrounds. In the wild-type L. lactis strain MG1363 growing on...... glucose, only small amounts of ethanol were obtained after introducing PDC, probably due to a low native alcohol dehydrogenase activity. When the same strains were grown on maltose, ethanol was the major product and lesser amounts of lactate, formate, and acetate were formed. Inactivating the lactate...... dehydrogenase genes ldhX, ldhB, and ldh and introducing codon-optimized Z. mobilis alcohol dehydrogenase (ADHB) in addition to PDC resulted in high-yield ethanol formation when strains were grown on glucose, with only minor amounts of by-products formed. Finally, a strain with ethanol as the sole observed...

  16. Efeito de um elixir com óleos essenciais e de um colutório com delmopinol nas bactérias da placa bacteriana

    OpenAIRE

    Luís, Henrique Soares, 1966-

    2010-01-01

    Tese de doutoramento, Ciências e Tecnologias da Saúde (Higiene Oral), Universidade de Lisboa, Faculdade de Medicina Dentária, 2011 Este trabalho de investigação teve por objectivo avaliar e comparar a eficácia de um elixir com óleos essenciais e de um colutório com delmopinol nas bactérias da placa bacteriana. Para tal foram realizados dois ensaios laboratoriais, dois ensaios clínicos e um estudo descritivo. Dos ensaios laboratoriais concluiu-se que a concentração mínima ini...

  17. A RIFAMPICINA NA DESCONTAMINAÇÃO BACTERIANA DE EXPLANTES DE MAMOEIRO PROVENIENTES DO CAMPO

    Directory of Open Access Journals (Sweden)

    GIOVANNI RODRIGUES VIANNA

    1997-01-01

    Full Text Available Observou-se alta contaminação bacteriana nos explantes de mamoeiro introduzidos in vitro, a partir de plantas matrizes desenvolvidas no campo, independentemente da época do ano em que se realizaram as coletas. O uso de desinfestantes superficiais, como álcool e hipoclorito de sódio, garantiram níveis aceitáveis de controle apenas para fungos, não para bactérias. A rifampicina, por tratamento de imersão ou introdução em meio de cultura, controlou satisfatoriamente as contaminações de caráter endofítico, obtendo-se 70% de explantes sadios, sem sinais de fitotoxicidade.High contamination by bacteria was observed in papaya tissue cuttings introduced in vitro from plants grown in the field, independent of the period of the year that samples were collected. The use of alcohol and sodium hypoclorite did not guarantee good bacteria control. Rifampicin, added as an immersion solution treatment or in the culture media, controlled the internal contamination of explants, without damaging the cuttings. Up to 70% of healthy tissue explants were obtained by the use of rifampicin.

  18. Eficacia de cinco desinfectantes para la reducción bacteriana doméstica

    Directory of Open Access Journals (Sweden)

    Julián Stambullian

    2011-06-01

    Full Text Available El uso adecuado de hipoclorito de sodio, sales de amonio cuaternario y triclosán ha demostrado ser eficaz para eliminar gérmenes dentro del hogar. Nuestro objetivo fue evaluar la eficacia inmediata, a la semana y al mes del uso controlado de cinco productos con estos componentes, comparados con otros productos de uso habitual. Se incluyeron 32 hogares de clase media de la Ciudad de Buenos Aires y la periferia en un estudio con intervención, abierto, aleatorizado, y de grupos paralelos, durante 6 meses. La mitad de los hogares fue seleccionada para usar hipoclorito de sodio, sales de amonio cuaternario y triclosán en la cocina y el baño durante un mes. El grupo control mantuvo prácticas habituales de limpieza doméstica. Se tomaron muestras para recuento y tipificación bacteriana de los sitios estudiados: muestras basales (sin discriminación de grupo en cocina, que presentaron recuento bacteriano promedio de 66.0 UFC/cm²; baño: 40.1 UFC/cm². Las muestras inmediatas a la limpieza (sin discriminación de grupo: en cocina: 0.8 UFC/cm²; baño: <1 UFC/cm². A la semana (grupo intervenido vs. grupo control: cocina 18.0 vs. 32.5 UFC/cm²; baño 12.7 vs. 7.7 UFC/cm². Al mes (intervenido vs. control: cocina: 60.1 vs. 62.1 UFC/cm²; baño: 37.0 vs. 42.0 UFC/cm². Se observó una notable disminución de la carga bacteriana en ambos grupos, lo que sugiere que no sólo la calidad de los productos sino también la educación en el uso desempeñan un papel clave en la desinfección del hogar. Este enfoque podría ser una herramienta importante para prevenir infecciones transmitidas por alimentos, dado que los coliformes fecales predominaron ampliamente en todas las muestras tipificadas.

  19. Actividad sialidasa en mujeres con vaginosis bacteriana Sialidase activity in women with bacterial vaginosis

    Directory of Open Access Journals (Sweden)

    Adriana M. Ombrella

    2006-04-01

    Full Text Available La vaginosis bacteriana (VB es un síndrome caracterizado por el sobrecrecimiento bacteriano de flora endógena Gram negativa, que desplaza a la flora lactobacilar normal. Dentro de las enzimas bacterianas, las sialidasas han sido consideradas factores de virulencia de muchos microorganismos patógenos que colonizan las distintas mucosas. Su presencia en fluidos vaginales puede estar correlacionada con VB. El propósito de este estudio fue comprobar la actividad de dicha enzima en mujeres con este síndrome y sin evidencia clínica de infección genital. Se estudiaron 112 mujeres (51 fueron pacientes con VB y 61 mujeres con flora colonizante habitual. Para la cuantificación de la actividad sialidasa se empleó la técnica basada en la hidrólisis enzimática de un derivado ácido del ácido metoxifenil acetil murámico. En la población estudiada se encontró que ambos grupos mostraron valores comprendidos entre 0.5 a 5.1 nmoles de metoxifenol, mientras que 11 de 52 pacientes con VB (21.17%, registraron valores superiores a 5.1 nmoles. La presencia de actividad sialidasa solamente no es índice de VB, excepto para valores mayores de 5.5 nmoles de metoxifenol, producidos en la reacción enzimática.Bacterial vaginosis (VB is a syndrome characterized by overgrowth of endogenous Gram negative bacterial flora and the lack of the normal flora. Within bacterial enzymes, sialidases have been considered a virulence factor of many pathogenic microorganisms colonizing the different mucous membranes. Their presence in vaginal discharges can be correlated with VB. The aim of this study was to detect the activity of this enzyme in women with this syndrome and without clinical evidence of genital infection. Out of a total 112 women studied, 51 were patients with VB and the other 61 women presented normal vaginal flora. For the quantification of enzyme activity, the technique based on the enzymatic hydrolysis of a derivative acid of the acetyl metoxifenil

  20. Murcha bacteriana no estado do Amazonas, Brasil Bacterial wilt in Amazonas State, Brazil

    Directory of Open Access Journals (Sweden)

    Rosalee A. Coelho Netto

    2004-02-01

    Full Text Available Durante 1998 e 2000, a incidência de murcha bacteriana causada por Ralstonia solanacearum foi registrada em 25 municípios do estado do Amazonas. A bactéria foi encontrada nas seguintes espécies vegetais: Capsicum annuum, C. chinense, C. frutescens, Cucumis sativus, Heliconia sp., Lycopersicon esculentum, Melanthera discoidea, Moringa oleifera, Musa sp., Solanum melongena, S. gilo, e S. nigrum. Em tomateiros (Lycopersicon esculentum, a murcha bacteriana estava presente em todos os plantios. Em bananeiras (Musa spp., a incidência do Moko foi menor nas várzeas dos rios Madeira e Negro do que nas dos rios Solimões e Amazonas. Caracterizaram-se 320 isolados de R. solanacearum, obtidos no levantamento, com relação a raça e a biovar. A biovar 1 predominou em todos os hospedeiros, com exceção de C. annuum e C. chinense, onde estirpes da biovar 3 foram maioria. Apenas 7,8% das estirpes foram da biovar N2. A sensibilidade de 56 estirpes da raça 1 a 23 bacteriocinas foi avaliada. As estirpes da biovar 3 apresentaram uma menor variabilidade, na sensibilidade a bacteriocinas do que as estirpes das biovares 1 e N2.A survey for bacterial wilt caused by Ralstonia solanacearum was conducted in 25 counties in the state of Amazonas during the years 1998 and 2000. Ralstonia solanacearum was found in the following species: Capsicum annuum, C. chinense, C. frutescens, Cucumis sativus, Heliconia sp., Lycopersicon esculentum, Melanthera discoidea, Moringa oleifera, Musa sp., Solanum melongena, S. gilo, and S. nigrum. In tomatoes (Lycopersicon esculentum, bacterial wilt was present in all fields. In banana (Musa spp., the incidence of Moko disease was lower in the flooded areas of the Madeira and Negro Rivers than in the Solimões and Amazonas Rivers. In this survey the race and biovar of 320 R. solanacearum isolates was determined. The biovar 1 strains predominate in almost all hosts. In C. annuum and C. chinense, however, biovar 3 was more common. Only 7

  1. Lactococcus nasutitermitis sp. nov. isolated from a termite gut.

    Science.gov (United States)

    Yan Yang, Shu; Zheng, Ying; Huang, Zhou; Min Wang, Xue; Yang, Hong

    2016-01-01

    Bacterial strain M19T was isolated from the gut of a wood-feeding termite, Nasutitermes hainanensis. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain M19T was related to members of the genus Lactococcus, with sequence similarities ranging from 84.8 to 95.5 %. Comparison of housekeeping gene ropB sequences revealed that strain M19T was well separated from Lactococcus fujiensis JCM 16395T and Lactococcus hircilactis 117T. The isolate was Gram-stain-positive, catalase-negative and non-motile. Cells were coccoid or ovoid-shaped, and occurred singly, in pairs or as short chains. Growth of strain M19T occurred at 10-40 °C and at pH 5.0-7.5. The DNA G+C content of strain M19T was 39.6 mol% and the major fatty acids were C16 : 0, cyclo-C19 : 0ω8c, C18 : 1ω9c, summed feature 7 and summed feature 8. Based on the phylogenetic, chemotaxonomic and phenotypic data presented, strain M19T represents a novel species of the genus Lactococcus, for which the name Lactococcus nasutitermitis sp. nov. is proposed. The type strain is M19T ( = CGMCC 1.15204T = NBRC 111537T). PMID:26546382

  2. INTERACCIONES ENTRE Gluconacetobacter diazotrophicus Y LA COMUNIDAD BACTERIANA ENDÓFITA DE LA CAÑA DE AZÚCAR

    Directory of Open Access Journals (Sweden)

    Marcia M. Rojas

    2007-01-01

    Full Text Available Gluconacetobacter diazotrophicus es una bacteria endófita de caña de azúcar, que se ha comprobado que es capaz de fijar cantidades considerables de nitrógeno y producir sustancias estimuladoras del crecimiento vegetal, lo cual puede ser de suma importancia en el mejoramiento de este cultivo sobre bases agroecológicas. En el presente trabajo se demuestran, por primera vez, las interacciones que esta especie establece con otros representantes de la comunidad bacteriana endófita de la caña de azúcar empleando el micrométodo en portaobjetos. G. diazotrophicus ejerce efecto antagonista sobre otros miembros de la comunidad, lo cual está influido por las cepas y el tiempo de crecimiento de la especie, que a su vez es estimulada por otras presentes en el interior de la caña de azúcar. Este hecho pudiera contribuir a potenciar la promoción del crecimiento que puede ejercer G. diazotrophicus en la caña de azúcar.

  3. Estudio de la expresión de enzimas del metabolismo de aminoácidos en lactococcus lactis

    OpenAIRE

    García-Cayuela, Tomás

    2011-01-01

    Los aminoácidos son fundamentales para la supervivencia y el desarrollo de bacterias. Son las principales fuentes de nitrógeno y están implicados en la producción de energía, el control del pH intracelular y la regeneración de cofactores. Además, son los precursores de una larga variedad de compuestos volátiles en Lactococcus lactis y, por ello, diversas enzimas son consideradas clave para su formación, como aminotransferasas, deshidrogenasas, liasas y decarboxilasas, entre otras. Estas enzim...

  4. Minimal requirements for exponential growth of Lactococcus lactis

    DEFF Research Database (Denmark)

    Jensen, Peter Ruhdal; Hammer, Karin

    1993-01-01

    A minimal growth medium containing glucose, acetate, vitamins, and eight amino acids allowed for growth of Lactococcus lactis subsp. lactis, with a specific growth rate in batch culture of mu = 0.3 h-1. With 19 amino acids added, the growth rate increased to mu = 0.7 h-1 and the exponential growt...

  5. Stability of Integrated Plasmids in the Chromosome of Lactococcus lactis

    NARCIS (Netherlands)

    Leenhouts, Kees J.; Kok, Jan; Venema, Gerhardus

    1990-01-01

    Derivatives of plasmids pBR322, pUB110, pSC101, and pTB19, all containing an identical fragment of lactococcal chromosomal DNA, were integrated via a Campbell-like mechanism into the same chromosomal site of Lactococcus lactis MG1363, and the transformants were analyzed for the stability of the inte

  6. pSEUDO, a Genetic Integration Standard for Lactococcus lactis

    NARCIS (Netherlands)

    Pinto, Joao P. C.; Zeyniyev, Araz; Karsens, Harma; Trip, Hein; Lolkema, Juke S.; Kuipers, Oscar P.; Kok, Jan

    2011-01-01

    Plasmid pSEUDO and derivatives were used to show that llmg_pseudo_10 in Lactococcus lactis MG1363 and its homologous locus in L. lactis IL1403 are suitable for chromosomal integrations. L. lactis MG1363 and IL1403 nisin-induced controlled expression (NICE) system derivatives (JP9000 and IL9000) and

  7. Autolysis of Lactococcus lactis is influenced by proteolysis

    NARCIS (Netherlands)

    Buist, G; Venema, G; Kok, J.

    1998-01-01

    The autolysin AcmA of Lactococcus lactis was shown to be degraded by the extracellular Lactococcal proteinase PrtP. Autolysis, as evidenced by reduction in optical density of a stationary-phase culture and concomitant release of intracellular proteins, was greatly reduced when L. lactis MG1363 cells

  8. Nisin-Producing Lactococcus lactis Strains Isolated from Human Milk

    OpenAIRE

    Beasley, Shea S.; Saris, Per E. J.

    2004-01-01

    Characterization by partial 16S rRNA gene sequencing, ribotyping, and green fluorescent protein-based nisin bioassay revealed that 6 of 20 human milk samples contained nisin-producing Lactococcus lactis bacteria. This suggests that the history of humans consuming nisin is older than the tradition of consuming fermented milk products.

  9. Nucleotide metabolism in Lactococcus lactis: Salvage pathways of exogenous pyrimidines

    DEFF Research Database (Denmark)

    Martinussen, Jan; Andersen, Paal Skytt; Hammer, Karin

    1994-01-01

    By measuring enzyme activities in crude extracts and studying the effect of toxic analogs (5-fluoropyrimidines) on cell growth, the metabolism of pyrimidines in Lactococcus lactis was analyzed. Pathways by which uracil, uridine, deoxyuridine, cytidine, and deoxycytidine are metabolized in L. lact...

  10. Structure-function analysis of multidrug transporters in Lactococcus lactis

    NARCIS (Netherlands)

    van Veen, HW; Putman, M; Margolles, A; Sakamoto, K; Konings, WN

    1999-01-01

    The active extrusion of cytotoxic compounds from the cell by multidrug transporters is one of the major causes of failure of chemotherapeutic treatment of tumor cells and of infections by pathogenic microorganisms. A multidrug transporter in Lactococcus lactis, LmrA, is a member of the ATP-binding c

  11. Multidrug transporters and antibiotic resistance in Lactococcus lactis

    NARCIS (Netherlands)

    Poelarends, GJ; Mazurkiewicz, P; Konings, WN

    2002-01-01

    The Gram-positive bacterium Lactococcus lactis produces two distinct multidrug transporters, designated LmrA and LmrP, that both confer resistance to a wide variety of cationic lipophilic cytotoxic compounds as well as to many clinically relevant antibiotics. While LmrP is a proton/drug antiporter t

  12. Production and secretion of heterologous proteins by Lactococcus lactis.

    NARCIS (Netherlands)

    Asseldonk, van M.

    1994-01-01

    Lactococcus lactis strains have been used for centuries in food fermentation, now appreciated as traditional biotechnology. They have been applied in the cheesemaking process and for the manufacturing of other dairy products. Years of experience with these lactic acid bacteria have led to a profound

  13. Perfil etiológico das meningites bacterianas em crianças Etiological profile of bacterial meningitis in children

    Directory of Open Access Journals (Sweden)

    Orlando C. Mantese

    2002-12-01

    Full Text Available Objetivo: analisar o perfil etiológico e alguns aspectos epidemiológicos das crianças com meningite bacteriana, internadas em um hospital público universitário. Métodos: foram seguidas, prospectivamente, as crianças internadas com meningite bacteriana, diagnosticada segundo os critérios clínicos e laboratoriais habituais. Foram excluídos os casos de meningite pós-trauma, de meningite na vigência de derivação liquórica, ou de defeitos congênitos do tubo neural, e de meningite tuberculosa. Resultados: foram analisadas 415 crianças internadas com diagnóstico de meningite bacteriana, no Hospital de Clínicas da Universidade Federal de Uberlândia, no período de 01/01/1987 a 31/01/2001. O agente etiológico foi identificado em 315 pacientes (75,9%, sendo de modo definitivo em 289 (69,3% e presuntivo, por intermédio da bacterioscopia, em outros 26 (6,6%. Os agentes mais comumente identificados foram o Haemophilus influenzae b (54,2%, o meningococo (20,6% e o pneumococo (18,1% dos 315 pacientes. O tratamento antimicrobiano prévio, detectado em 47,2% dos casos, causou uma diminuição significante no rendimento das culturas de sangue (de 50,8% para 38,7% e de liquor (71,7% para 57,6%. Houve um predomínio do acometimento de crianças com idade até 48 meses pelo Haemophilus influenzae b, particularmente em relação ao meningococo. A letalidade geral foi de 10,1%, com diferença significante entre a letalidade do pneumococo, de 17,5%, e a do meningococo, de 4,6%. Conclusões: as crianças afetadas por Haemophilus influenzae b e por pneumococo foram mais jovens que aquelas com meningite por meningococo. A hemocultura e a cultura de liquor continuam sendo importantes recursos laboratoriais para o diagnóstico etiológico da meningite bacteriana, apesar do impacto negativo causado em seu rendimento pelo tratamento antibiótico prévio. Os agentes mais comumente isolados foram o Haemophilus influenzae b, o meningococo e o pneumococo. A

  14. La placa bacteriana: conceptos básicos para el higienista bucodental

    OpenAIRE

    Poyato Ferrera, Manuel María; Segura-Egea, Juan J; Ríos-Santos, J.V.; Bullon, Pedro

    2001-01-01

    La placa bacteriana es el factor etiológico principal de las dos enfermedades bucodentales de mayor prevalencia, la caries y la enfermedad periodontal. El higienista bucodental y colaborador del odontoestomatólogo en la aplicación del programa de control de placa, debe conocer en profundidad la microbiología de la placa bacteriana dental, así como los mecanismos implicados en su patogenicidad cariogénica y periodontal, aspectos que se revisan a continuación. Dental plaque is the main etiol...

  15. IDENTIFICACIÓN MOLECULAR DE POBLACIONES BACTERIANAS ASOCIADAS AL CARACOL PALA (Strombus gigas DEL CARIBE COLOMBIANO

    Directory of Open Access Journals (Sweden)

    ROMERO MAGALLY

    2009-08-01

    Full Text Available RESUMEN

    El caracol Pala, Strombus gigas (Strombidae, es de gran importancia ecológica y socioeconómica en el área caribeña colombiana. Sin embargo, es una especie catalogada como “vulnerable” y existe muy poca información referente a las especies bacterianas asociadas al caracol que puedan ser importantes para el desarrollo, manejo productivo y de seguridad acuícola de estos gastrópodos. En este trabajo, nosotros empleamos un estudio microbiológico y molecular de la región intergénica entre los genes 16S y 23S rDNA, análisis del gen rDNA 16S y secuenciación, para analizar las bacterias asociadas al caracol Pala (S. gigas. La composición de bacterias cultivables asociadas fue evaluada por su capacidad para crecer en agar marino y en medios de cultivos selectivos. De un total de 28 muestras analizadas encontramos que el número de bacterias cultivadas en condiciones aerobias fue de alrededor 106 ufc mL-1 donde las bacterias pertenecientes a la familia Vibrionacea fueron las más abundantes, cerca de >105 ufc mL-1 . El análisis molecular de la región intergénica entre los genes 16S y 23S rDNA de las diferentes muestras, reveló una gran complejidad bacteriana asociada a S. gigas. Las secuencias de los amplificados del gen rDNA 16S identificó Pseudoalteromonas sp., Halomonas sp., Psycrobacter sp., Cobetia sp., Pseudomonas sp. y Vibrios sp. Nuestros resultados podrían sugerir un rol importante de estas bacterias como componentes de la comunidad asociada al S. gigas. Esta información puede complementar los estudios que se están implementando en los procesos para la conservación y repoblamiento de las poblaciones de S. gigas en Colombia.

    Palabras clave: Strombus gigas, Caracol pala, Bacteria, Región intergénica 16S-23S, rDNA 16S.

    ABSTRACT

    The Queen Conch, Strombus gigas (Strombidae, is a species of great ecological and socioeconomic importance in the Caribbean area of Colombia

  16. RELACIÓN DEL NITRATO SOBRE LA CONTAMINACIÓN BACTERIANA DEL AGUA

    Directory of Open Access Journals (Sweden)

    Silvia Rodr\\u00EDguez

    2012-01-01

    Full Text Available La contaminación de aguas subterráneas con nitrato constituye uno de los mayores problemas a nivel mundial. En Argentina, concentraciones mayores a 45 mg L-1 son potencialmente peligrosas y se han encontrado en aguas para consumo humano, concentraciones mayores a dicho umbral. Hay antecedentes que mencionan la existencia de una relación entre las concentraciones de este ión y la proliferación de bacterias. Para determinar la capacidad indicativa del nitrato en la contaminación bacteriana de aguas en distintas fuentes de agua de Itatí, Corrientes, Argentina, se seleccionaron doce puntos de muestreo. Se efectuaron, quincenalmente, durante siete meses, análisis físico-químicos y bacteriológicos. Las concentraciones de nitrato se determinaron por espectrofotometría de absorción molecular, el recuento de bacterias coliformes se realizó mediante técnica del número más probable (NMP en caldo Mac Conkey y confirmación de positivos con caldo Brilla a 37 °C, las coliformes fecales se determinaron a partir de los positivos de la fase presuntiva y las coliformes no fecales por siembra en citrato de Koser. Los datos fueron sometidos a un análisis de estadística descriptiva. Las relaciones entre los parámetros estudiados fueron analizadas mediante correlación de Pearson empleando programa estadístico Infostat. Las concentraciones de nitrato en el agua superficial no superaron los 4 mg L-1, en todos los puntos de muestreo mientras que en las perforaciones los valores oscilaron entre 0.05 a 36 mg L-1 y en los pozos entre 0.32 y 37 mg L-1. La mayor cantidad de coliformes totales detectadas, corresponden a las muestras de agua de pozo y laguna. Tanto en aguas superficiales como subterráneas se encontraron coliformes fecales. Se encontraron correlaciones negativas significativamente entre nitrato y coliformes totales y nitrato y coliformes no fecales. El origen de la contaminación puede atribuirse a fuentes localizadas y no a la actividad

  17. Ação do pneumoperitônio com dióxido de carbono na translocação bacteriana em ratos

    Directory of Open Access Journals (Sweden)

    Nemer Hajar

    2002-05-01

    Full Text Available Objetivo: Investigar a translocação bacteriana decorrente da ação do pneumoperitônio com CO2 em ratos normais e em ratos com colite induzida. Métodos: Utilizaram-se 60 ratos distribuídos em seis grupos de 10 animais. No grupo simulação realizou-se laparotomia, manipulação de órgãos abdominais e síntese da parede; os dois grupos pneumoperitônio foram submetidos a pneumoperitônio por uma e três horas, respectivamente; no grupo colite, foi induzida colite e realizada laparotomia, manipulação de órgãos abdominais e síntese da parede; nos dois grupos colite pneumoperitônio induziu-se colite mais pneumoperitônio por uma e três horas, respectivamente. Sacrificados os animais, foram feitas as coletas dos rins, baço, fígado e linfonodos mesentéricos. Nos animais submetidos a colite induzida, foram ressecados 2cm de cólon para análise histológica. Nos órgãos ressecados pesquisou-se a presença de Escherichia coli e Enterococcus faecallis para verificar a translocação bacteriana. Resultados: Não ocorreram óbitos nos grupos estudados. Não houve diferença estatisticamente significante nos grupos de animais submetidos a colite. A ocorrência de translocação bacteriana nos diferentes órgãos independe do gênero da bactéria. Não há incremento da translocação bacteriana com relação a duração de pneumoperitônio (1 e 3h tanto em ratos normais como nos submetidos a colite induzida. Conclusão: O pneumoperitônio induz a translocação bacteriana em ratos normais e a incrementa em ratos submetidos à colite.Objective: This study has the purpose of determining if pneumoperitoneum with CO2 develops bacterial translocation in normal rats and in rats submitted to induced colitis. Methods: 60 rats, divided into six groups of 10 animals, were utilized. In the sham group, laparotomy, abdominal organ manipulation, and suture of the wall were performed; the two pneumoperitoneum groups were submitted to pneumoperitoneum for

  18. Taxonomy, physiology and growth of Lactococcus lactis: a review

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    Dubravka Samaržija

    2001-01-01

    Full Text Available Lactococcus lactis species is one of the most important groups of lactic acid bacteria that are used in the dairy industry. The major functions of this species in dairy fermentation are the production of lactic acid from lactose, hydrolysis of casein and citric acid fermentation. Thus their metabolic end products and enzymes directly or indirectly have significant influence in determining the texture and flavour of the final products. In recent years, genetics and physiological properties of lactococci have considerable changed. Therefore, both for basic research and for application purposes in this paper the general view of the new taxonomic classification of Lactococcus lactis, the role of their plasmids and the physiology and nutritional requirements during growth are discussed.

  19. Production and secretion of heterologous proteins by Lactococcus lactis.

    OpenAIRE

    Asseldonk, van, M.

    1994-01-01

    Lactococcus lactis strains have been used for centuries in food fermentation, now appreciated as traditional biotechnology. They have been applied in the cheesemaking process and for the manufacturing of other dairy products. Years of experience with these lactic acid bacteria have led to a profound understanding of the microbiological and technological aspects of L.lactis. Recent progress in the genetics of L. lactis made this organism a suitable candidate for the use in modern biotechnology...

  20. Lactococcus garvieae Endocarditis on a Prosthetic Biological Aortic Valve.

    Science.gov (United States)

    Tsur, A; Slutzki, T; Flusser, D

    2015-09-01

    Lactococcus garvieae (LG) endocarditis is a rare disease in humans. There are only about 16 reported cases in the world. We report a 76-year-old male patient with LG endocarditis. In depth interview with the patient revealed that 2 weeks prior to admission, he had eaten sushi containing raw fish. Unlike many of the other infections reported, which were on a native mitral valve, our patient's vegetation was on a prosthetic aortic valve. PMID:25295408

  1. Taxonomy, physiology and growth of Lactococcus lactis: a review

    OpenAIRE

    Dubravka Samaržija; Neven Antunac; Jasmina Lukač Havranek

    2001-01-01

    Lactococcus lactis species is one of the most important groups of lactic acid bacteria that are used in the dairy industry. The major functions of this species in dairy fermentation are the production of lactic acid from lactose, hydrolysis of casein and citric acid fermentation. Thus their metabolic end products and enzymes directly or indirectly have significant influence in determining the texture and flavour of the final products. In recent years, genetics and physiological properties of ...

  2. Expression of the Staphylococcus hyicus Lipase in Lactococcus lactis

    OpenAIRE

    Drouault, Sophie; Corthier, Gerard; Ehrlich, S. Dusko; Renault, Pierre

    2000-01-01

    The extracellular Staphylococcus hyicus lipase was expressed under the control of different promoters in Lactococcus lactis and Bacillus subtilis. Its expression at high and moderate levels is toxic for the former and the latter hosts, respectively. In L. lactis, the lipase was expressed at a high level, up to 30% of the total cellular proteins, under the control of the inducible promoter PnisA. About 80% of the lipase remained associated with the cells. Close to half of this amount remained ...

  3. Relationship between Glycolysis and Exopolysaccharide Biosynthesis in Lactococcus lactis

    OpenAIRE

    Ramos, Ana; Boels, Ingeborg C.; de Vos, Willem M; Santos, Helena

    2001-01-01

    The relationships between glucose metabolism and exopolysaccharide (EPS) production in a Lactococcus lactis strain containing the EPS gene cluster (Eps+) and in nonproducer strain MG5267 (Eps−) were characterized. The concentrations of relevant phosphorylated intermediates in EPS and cell wall biosynthetic pathways or glycolysis were determined by 31P nuclear magnetic resonance. The concentrations of two EPS precursors, UDP-glucose and UDP-galactose, were significantly lower in the Eps+ strai...

  4. Molecular Physiology of Sugar Catabolism in Lactococcus lactis IL1403

    OpenAIRE

    Even, Sergine; Lindley, Nic D.; Cocaign-Bousquet, Muriel

    2001-01-01

    The metabolic characteristics of Lactococcus lactis IL1403 were examined on two different growth media with respect to the physiological response to two sugars, glucose and galactose. Analysis of specific metabolic rates indicated that despite significant variations in the rates of both growth and sugar consumption, homolactic fermentation was maintained for all cultures due to the low concentration of either pyruvate-formate lyase or alcohol dehydrogenase. When the ionophore monensin was add...

  5. Relationship between glycolysis and exopolysaccharide biosynhesis in Lactococcus lactis

    OpenAIRE

    Ramos, A.; Boels, I. C.; Vos; Santos, H.

    2001-01-01

    The relationships between glucose metabolism and exopolysaccharide (EPS) production in a Lactococcus lactis strain containing the EPS gene cluster (Eps ) and in nonproducer strain MG5267 (Eps) were characterized. The concentrations of relevant phosphorylated intermediates in EPS and cell wall biosynthetic pathways or glycolysis were determined by 31P nuclear magnetic resonance. The concentrations of two EPS precursors, UDP-glucose and UDP-galactose, were significantly lower in the Eps strain ...

  6. Ação do biofertilizante Agrobio sobre a mancha-bacteriana e desenvolvimento de mudas de pimentão Effect of the Agrobio biofertilizer on the bacterial spot and the development of bell pepper transplants

    OpenAIRE

    Cláudia S.R. Deleito; Margarida Goréte F. do Carmo; Maria do Carmo A Fernandes; Antonio Carlos de S. Abboud

    2005-01-01

    O biofertilizante Agrobio é produzido a partir de esterco bovino fresco, água, melaço e sais minerais em recipientes abertos. Tem sido largamente utilizado por agricultores orgânicos e convencionais no estado do Rio de Janeiro, ao qual atribuem efeito nutricional e de controle de doenças. O presente trabalho foi feito com o objetivo de elucidar e quantificar aspectos relativos ao efeito do Agrobio sobre o controle da mancha-bacteriana e desenvolvimento de mudas de pimentão, em condições de ca...

  7. Evaluación preliminar de las poblaciones bacterianas asociadas al tracto intestinal de la tilapia (Oreochromis niloticus) expuesta a aceites esenciales de orégano en la dieta

    OpenAIRE

    Núñez De La Rosa, Melisa Giset

    2011-01-01

    El efecto de los aceites esenciales orégano (AEO) sobre la diversidad bacteriana del tracto intestinal (TI) de Oreochromis niloticus fue evaluado por la suplementación con diferentes relaciones de carvacrol:timol en la dieta. El estudio se realizó en la estación piscícola la Terraza (Villavicencio). Los peces fueron alimentados con una dieta basal con 200 ppm de AEO. El TI fue muestreado de 24 tilapias adultas, seguido de la extracción del ADN total usando un protocolo modificado, la amplific...

  8. Contagem de morfotipos de Mobiluncus sp e concentração de leucócitos em esfregaços vaginais de pacientes com vaginose bacteriana

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    Eleutério Junior José

    2004-01-01

    Full Text Available OBJETIVOS: pesquisar em casos de vaginose bacteriana a presença de bacilos curvos, sugerindo Mobiluncus, e de leucocitose em esfregaços vaginais corados por Gram e Papanicolaou. MÉTODOS: duzentos e cinco pacientes com diagnóstico de vaginose bacteriana pelos Critérios de Amsel foram incluídas e avaliadas clinicamente por um dos autores (JEJ. Os esfregaços vaginais foram corados pelo método de Papanicolaou e avaliados pela técnica de Gram. Nos esfregaços de Gram foram estudados o escore de Nugent e a presença de bacilos curvos. Nos esfregaços de Papanicolaou foi avaliada a presença de bacilos curvos e de leucócitos. Os dados foram analisados usando Prism 3.0® com intervalo de confiança de 95%, usando teste exato de Fisher pelo método modificado de Wald. RESULTADOS: nos esfregaços de Papanicolaou, bacilos curvos estiveram presentes em 51,7% das vezes. O número de leucócitos variou, embora a presença de poucos leucócitos tenha sido mais freqüente. Nos esfregaços de Gram, bacilos curvos foram observados em 46,8% e o escore de Nugent foi 8 em 48,3% dos casos. O achado de Mobiluncus sp pelos dois métodos ocorreu em 82%. Na presença de bacilos curvos, maior número de leucócitos foi observado em 57,5% dos casos (p <0,0001 e quando o escore de Nugent foi de 9 e 10, a leucocitose ocorreu em 25,6 e 37,2% das vezes, respectivamente. CONCLUSÕES: bacilos curvos são observados, morfologicamente, em cerca de 52% dos casos de vaginose bacteriana. Leucocitose é mais observada na presença de Mobiluncus sp e em casos com escores de Nugent mais altos.

  9. Meningencefalites bacterianas agudas em crianças: complicações e sequelas neurológicas

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    NATALINO WLADIMIR

    1999-01-01

    Full Text Available Foram estudadas 271 crianças com idade inferior a 15 anos, com diagnóstico de meningencefalite bacteriana aguda (MBA, no período 1980 -- 1990. Os pacientes foram divididos em dois grupos, conforme não tivessem recebido tratamento prévio (STP, 153 casos, e os que receberam tratamento prévio (CTP, 118 casos. Ocorreram diferenças significativas em relação a identificação do agente etiológico, que foi maior no grupo STP, e em relação a ocorrência de ventriculite, maior no grupo CTP. Ocorreu óbito em 19,5% dos 271 casos, com predomínio em crianças de idade abaixo de 12 meses (29,7% e nos pacientes com MBA por Streptococcus pneumoniae. As manifestações epilépticas e as paresias na fase aguda da MBA predominaram em crianças de idade abaixo de 1 ano. Foram identificados e tratados do ponto de vista neurocirúrgico as ventriculites, higroma subdural, hidrocefalia, empiema subdural e abscesso cerebral.

  10. Importancia clínica de la translocación bacteriana Clinical importance of bacterial translocation

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    A. García de Lorenzo y Mateos

    2007-05-01

    Full Text Available El tracto gastrointestinal está colonizado por una inmensa cantidad de microorganismos, a los que denominamos flora intestinal. Aunque el intestino proporciona una barrera funcional entre estos organismos y el huésped, la traslocación bacteriana no es un evento infrecuente entre las personas sanas. Sin embrago, en los pacientes críticamente enfermos, que son portadores de diferentes entidades nosológicas, la translocación bacteriana puede condicionar infecciones e incrementos en la morbi-mortalidad. Varios son los mecanismos propuestos para explicar la etiología, génesis y vías de esta entidad y, frecuentemente, los resultados de las investigaciones realizadas tanto in vitro como en animales de experimentación son controvertidos y de difícil aplicación a los humanos. Muchas enfermedades se han relacionado o están implicadas en el fenómeno de translocación pero desgraciadamente el sumatorio de estudios metodológicamente insuficientes, poblaciones reducidas y resultados conflictivos dejan, hoy en día, muchas preguntas abiertas y otras sin respuesta lógica. Esta problemática también se refleja a la hora de estudiar y valorar las diferentes estrategias terapéuticas utilizadas así como los métodos empleados para detectar la traslocación.The gastrointestinal tract is colonized by a huge number of microorganisms that we call intestinal flora. Although the bowel provides a functional barrier between these organisms and the host, bacterial translocation is not an infrequent event in healthy people. However, in critically ill patients, carriers of different morbid entities, bacterial translocation may favor infections and increased morbimortality. There are several proposed mechanisms explaining the etiology, genesis, and ways for this entity, and frequently the results from both in vitro and animal experimental investigations are controversial and difficult to apply to humans. Many diseases have been linked or are implicated in

  11. Fermentation Tecniques and Applications of Bacterial Cellulose: a Review Técnicas de fermentación y aplicaciones de la celulosa bacteriana: una revisión

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    Luz Dary Carreño Pineda

    2012-12-01

    Full Text Available Bacterial cellulose is a polymer obtained by fermentation with microorganismsfrom Acetobacter, Rhizobium, Agrobacterium and Sarcina genera. Amongthem, Acetobacter xylinum is the most efficient specie. This polymer hasthe same chemical composition of plant cellulose, but its conformation andphysicochemical properties are different, making it attractive for several applications, especially in the areas of food, separation processes, catalysis andhealth, due to its biocompatibility. However, the main problem is the production in mass that is constrained by low yield. It is therefore necessaryto develop some alternatives. This paper presents a review about synthesis,production, properties and principal applications of bacterial cellulose, as wellas some alternatives to reduce the difficulties for process scaling.La celulosa bacteriana es un polímero obtenido por fermentación con microrganismosde los géneros Acetobacter, Rhizobium, Agrobacterium y Sarcina, delas cuales la especie más eficiente es la Acetobacter Xylinum. Este polímero presenta la misma estructura química de la celulosa de origen vegetal, pero difiereen su conformación y propiedades fisicoquímicas, lo que lo hace atractivo para diversas aplicaciones, especialmente en las áreas de alimentos, procesosde separación, catálisis y en medicina, gracias a su biocompatibilidad. Sin embargo, el principal problema es la producción a gran escala limitada por losbajos rendimientos, lo que genera la necesidad de desarrollar alternativas que permitan disminuir o eliminar las causas de esta limitación. En este artículo se hace una revisión acerca de la síntesis, producción, propiedades y principales aplicaciones de la celulosa bacteriana, así como de algunas alternativas estudiadas para disminuir los inconvenientes en el escalamiento del proceso.

  12. Plaque removal by manual and electric toothbrushing among children Remoção de placa bacteriana pela escovação elétrica e manual em crianças

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    Carolina Covolo da COSTA

    2001-12-01

    procedimentos foram realizados. De acordo com os resultados, não houve diferença estatisticamente significante na remoção de placa bacteriana quando as escovas foram usadas por crianças em fase de dentição mista. No entanto, a escova elétrica removeu significativamente mais placa bacteriana nas faces linguais das crianças com dentição decídua.

  13. Genome Sequence of Lactococcus garvieae 8831, Isolated from Rainbow Trout Lactococcosis Outbreaks in Spain▿

    OpenAIRE

    Aguado-Urda, Mónica; López-Campos, Guillermo H.; Gibello, Alicia; Cutuli, M. Teresa; López-Alonso, Victoria; Fernández-Garayzábal, José F.; Blanco, M. Mar

    2011-01-01

    Lactococcus garvieae is the etiological agent of lactococcosis, one of the most important disease threats to the sustainability of the rainbow trout farming industry. Here, we present the draft genome sequence of Lactococcus garvieae strain 8831, isolated from diseased rainbow trout, which is composed of 2,087,276 bp with a G+C content of 38%.

  14. Genome Sequence of a Lactococcus lactis Strain Isolated from Salmonid Intestinal Microbiota.

    Science.gov (United States)

    Opazo, Rafael; Gajardo, Felipe; Ruiz, Mauricio; Romero, Jaime

    2016-01-01

    Lactococcus lactis is a common inhabitant of the intestinal microbiota of salmonids, especially those in aquaculture systems. Here, we present a genome sequence of a Lactococcus lactis strain isolated from the intestinal contents of rainbow trout reared in Chile. PMID:27563049

  15. Associação da Vaginose Bacteriana com o Parto Prematuro Espontâneo Association of Bacterial Vaginosis with Spontaneous Preterm Delivery

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    Mário Henrique Burlacchini de Carvalho

    2001-09-01

    Full Text Available Objetivo: relacionar a presença de vaginose bacteriana em gestantes com a ocorrência de parto prematuro espontâneo. Método: foram estudadas 611 gestantes do serviço pré-natal da Clínica Obstétrica do HCFMUSP. Foram incluídas gestantes com idade gestacional confirmada por ultra-sonografia no primeiro trimestre de gestação ou duas ultra-sonografias até a 20ª semana quando havia discordância da idade gestacional no primeiro exame. Os partos prematuros eletivos foram excluídos. A pesquisa da vaginose foi realizada na 23ª ou 24ª semana de gestação por meio da técnica de coloração de Gram. O pH vaginal foi pesquisado utilizando-se fita de pH Universal 0-14 produzida pela Merck. O conteúdo vaginal foi coletado com a paciente em posição ginecológica, utilizando-se espéculo não-lubrificado. O pH foi medido na parede lateral da vagina e o conteúdo para Gram foi coletado do fundo de saco vaginal utilizando-se de cotonete estéril. Resultado: das 611 gestantes envolvidas inicialmente no estudo, foram obtidos os resultados do parto em 541. A vaginose bacteriana foi diagnosticada por bacterioscopia em 19% dos casos. No grupo de gestantes com vaginose, 9,7% (10/103 evoluíram com parto prematuro, contra apenas 3,2% (14/438 no grupo negativo (p=0,008. A sensibilidade da bacterioscopia positiva para vaginose bacteriana para predição do parto prematuro foi de 41,7%, a especificidade de 82%, a acurácia de 80,2%, com taxa de falso-positivos de 18% e risco relativo de 1,8. Conclusão: a vaginose bacteriana diagnosticada pelo Gram do conteúdo vaginal representa fator de risco para o parto prematuro, com risco relativo de 1,8.Purpose: to evaluate the relationship between bacterial vaginosis (BV and spontaneous preterm delivery. Method: a total of 611 pregnant women from the general antenatal clinic of the "Clínica Obstétrica do Hospital das Clínicas da Universidade de São Paulo" were enrolled in this study. All pregnancies were

  16. PATOGENICIDAD BACTERIANA EN MAÍZ (ZEA MAYS

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    Rocío Pérez-y-Terrón

    2012-01-01

    Full Text Available El maíz es un grano de consumo mundial para alimento tanto humano como animal. México se encuentra en el séptimo lugar de la producción, China y Estados Unidos son los principales consumidores. Zea mays pertenece a la familia de las gramíneas y tiene producción anual. La planta se puede ver afectada por plagas principalmente de diferentes especies de insectos. Puede también desarrollar enfermedad debida a hongos y virus, así como a bacterias patogénicas. Dentro de estas la causada por organismos del género Pantoea. P. stewartii se sabe que tiene genes como cps que codifican para la producción de exopolisacarido sterwatan controlada por un mecanismo de quórum sensing, y hrp para el sistema de secreción tipo III involucrados en la patogenicidad de la bacteria.

  17. PATOGENICIDAD BACTERIANA EN MAÍZ (ZEA MAYS)

    OpenAIRE

    Rocío Pérez-y-Terrón; Julio César Carmona Díaz; Jorge Alejandro Cebada Ruíz; José Antonio Munive Hernández

    2012-01-01

    El maíz es un grano de consumo mundial para alimento tanto humano como animal. México se encuentra en el séptimo lugar de la producción, China y Estados Unidos son los principales consumidores. Zea mays pertenece a la familia de las gramíneas y tiene producción anual. La planta se puede ver afectada por plagas principalmente de diferentes especies de insectos. Puede también desarrollar enfermedad debida a hongos y virus, así como a bacterias patogénicas. Dentro de estas la causada por organis...

  18. Bacterial diversity in the sediments of a temperate artificial lake, Rapel reservoir Diversidad bacteriana en sedimentos de un lago artificial temperado, embalse Rapel

    Directory of Open Access Journals (Sweden)

    CRISTINA DORADOR

    2007-06-01

    Full Text Available Rapel reservoir is an eutrophic system in Chile that has undergone a series of anthropogenic impacts in recent decades. To provide a better understanding of the processes occurring in the reservoir, we examined bacterial composition from surface sediments using traditional microbiology techniques and molecular biology tools. Our results showed significant temporal variation in the physical and chemical composition of the water column, but no depth-related differences during the study period. To detect temporal changes in bacterial composition, cultivable heterotrophic bacteria, heterotrophic iron oxidizing bacteria, and sulfate-reducing bacteria were extracted from the surface sediments and their concentration measured. Microbial diversity in sediments was represented by closest relatives of eight different bacterial phyla. The most frequently recovered phylotypes in the clone library of 16S rDNA were related to sulfate-reducing bacteria belong to the Deltaproteobacteria groupEl embalse Rapel es un sistema eutrófico en Chile que ha experimentado diversos impactos antropogénicos en las últimas décadas. Para conocer mejor los posibles procesos que ocurren en el embalse, la composición bacteriana fue estudiada en la zona superficial de los sedimentos utilizando técnicas microbiológicas tradicionales y herramientas de biología molecular. Nuestros resultados mostraron variación temporal significativa en la composición física y química del agua, no encontrándose diferencias de las variables medidas entre superficie y fondo en la columna de agua durante el período de estudio. Para detectar cambios temporales en la concentración y composición bacteriana, se midieron bacterias heterótrofas, heterótrofas oxidantes de fierro y bacterias reductoras de sulfato extraídas de la superficie de los sedimentos. La diversidad bacteriana en los sedimentos estuvo representada por 8 diferentes Phyla del dominio Bacteria. Los filotipos m

  19. Regulation of Proteolytic Enzyme Activity in Lactococcus lactis

    OpenAIRE

    Meijer, W.; Marugg, J D; Hugenholtz, J

    1996-01-01

    Two different Lactococcus lactis host strains, L. lactis subsp. lactis MG1363 and L. lactis subsp. cremoris SK1128, both containing plasmid pNZ521, which encodes the extracellular serine proteinase (PrtP) from strain SK110, were used to study the medium and growth-rate-dependent activity of three different enzymes involved in the proteolytic system of lactococci. The activity levels of PrtP and both the intracellular aminopeptidase PepN and the X-prolyl-dipeptidyl aminopeptidase PepXP were st...

  20. Infecções bacterianas pioram o prognóstico da hepatite alcoólica Alcoholic hepatitis: bad prognosis due to concomitant bacterial infections

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    Edna Strauss

    2004-06-01

    Full Text Available As infecções bacterianas cursam com altos índices de morbilidade e mortalidade na cirrose hepática. O objetivo do nosso trabalho foi avaliar se também na hepatite alcoólica as infecções bacterianas são fatores de mau prognóstico. Na avaliação retrospectiva de 681 pacientes hospitalizados em um único centro, por período de 6 anos, foram bem documentados 52 (7,5% casos de hepatite alcoólica, sendo 73,1% com biópsia hepática para análise histopatológica e os restantes por diagnóstico clínico-bioquímico. Houve predomínio do sexo masculino (relação 3,3:1,0, com idade média de 40 anos e ingestão média de etanol puro de 193g/dia por mais de 3 anos. As principais complicações foram: encefalopatia hepática (n=5, insuficiência renal (n=4 e hemorragia digestiva alta (n=3. Houve infecção bacteriana em 11 (21,1% pacientes, sendo pulmonar (n=5, peritonite bacteriana espontânea (PBE (n=2, urinária (n=3 e dermatológica (n=1. Óbito precoce, durante o período de internação ocorreu em 8 (15,4% casos e a análise comparativa entre eles e os sobreviventes mostrou serem fatores de mau prognóstico a presença de encefalopatia hepática (p=0,012, bilirrubinas > 20mg% (p=0,012 e associação com infecções graves (pulmonar/PBE, com p=0,004. Em conclusão, demonstramos que as infecções bacterianas são fatores de mau prognóstico na hepatite alcoólica. Recomendamos, portanto, que a profilaxia com antibióticos que se faz durante hemorragia digestiva alta na cirrose e em casos de insuficiência hepática fulminante, seja estendida para a hepatite alcoólica, em sua forma grave, com finalidade de evitar infecções bacterianas e mortalidade precoce.Bacterial infections increase morbidity and mortality in cirrhosis. Our aim was to investigate whether in alcoholic hepatitis the development of bacterial infections was also a poor prognostic factor. In the retrospective evaluation of 681 hospitalized patients with liver disease

  1. Aislamiento y selección de una cepa bacteriana degradadora de hidrocarburos a partir de suelos contaminados con petróleo

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    Rosa María Pérez Silva

    2008-01-01

    Full Text Available El conocimiento científico acerca del papel que desempeñan los microorganismos en el tratamiento de agentes contaminantes del medio ambiente como el petróleo, es esencial para prevenir y controlar los daños que puedan ocasionar los derrames o fugas de estos contaminantes. La degradación de petróleo es un proceso que puede ocurrir de forma natural por los microorganismos nativos de las zonas contaminadas aprovechando sus rutas metabólicas. Por esta razón, en estos momentos se prevé que los microorganismos pueden ofrecer esta posibilidad en tecnologías basadas en el uso de estos en la remediación de la contaminación ambiental por petróleo y sus derivados. En el presente trabajo, se estudió la biodegradación de petróleo por cepas aisladas de suelos contaminados con petróleo, mediante un aislamiento realizado por enriquecimiento secuencial utilizando petróleo Mesa 30/Puerto escondido (80 : 20 como única fuente de carbono y energía. Se aislaron 9 cepas bacterianas, cinco Gram negativas y cuatro Gram positivas, que fueron identificadas según el Manual Bergey's, 1994. Las pruebas bioquímicas evaluadas confirmaron que las cepas aisladas AT14, AT15, AT16, AT17 y AT18 corresponden a Pseudomonas aeruginosa. En este estudio, se seleccionó la cepa Pseudomonas aeruginosa AT18, por mostrar mayor crecimiento sobre petróleo como única fuente de carbono y energía al obtenerse 1,83 g/L de biomasa celular, lo que representa un 57 % de biodegradación de petróleo.

  2. Molecular characterization of bacterial populations of different soils Caracterização molecular de populações bacterianas de diferentes solos

    Directory of Open Access Journals (Sweden)

    Rodrigo Matheus Pereira

    2006-12-01

    por PCR, amplicons foram clonados e 139 clones de duas bibliotecas foram seqüenciados. O uso da técnica de 16S rRNA, gerou a identificação de diferentes populações de bactérias de solo pertencentes aos filos Acidobacteria Actinobacteria Bacteroidetes Firmicutes Proteobacteria Verrucomicrobia, Archaea, além das não classificadas. Diferenças entre as bibliotecas FS e CS foram observadas no tamanho dos filos. Um grande número de filos e, consequentemente, uma grande diversidade bacteriana foi observada no solo sob floresta. Estes dados foram confirmados pela análise de diversidade genética realizada. A caracterização de comunidades do solo apresentada neste trabalho contribuiu fornecendo dados para estudos posteriores sobre a dinâmica das populações bacterianas em solos de diferentes condições no Brasil.

  3. Validación de la prueba de Papanicolaou en el diagnóstico de vaginosis bacteriana. Antioquia, Colombia

    Directory of Open Access Journals (Sweden)

    María Elena Vásquez Peña

    2002-01-01

    Full Text Available A descriptive cross sectional study was carry out in 423 women in seven rural communities of the municipality of Amagá, Antioquia. One of the objectives of this study was to evaluate the sensibility and specificity and predictive values of the test of Papanicolaou in relation to the Gram exam, for the diagnosis of Bacterial Vaginosis (BV. In the 423 studied women the results showed a prevalence of BV for the bacteriological exam Gram of 20.8 % (88 cases and for Papanicolaou of 21% (89 cases. The sensitivity was 87.5%, the specificity was 96.4%. The value predictive positive was 86,5% and the value predictive negative was 96.7%. The high sensitivity, specificity and predictive values of the Papanicolaou test reported in this study make it a good screening method, since it is an exam that is carried out commonly and no additional testa are required. Se realizó un estudio descriptivo de corte en 423 mujeres de la población rural del municipio de Amagá, Antioquia, cuyas edades estaban comprendidas entre los 15 y 65 años, a quienes se les tomó muestras cérvicovaginales para estudio citológico de Papanicolau y coloración de Gram. El objetivo de este estudio fue evaluar la sensibilidad, especificidad y valores predictivos de la prueba de Papanicolaou frente al Gram, para el diagnóstico de vaginosis bacteriana (vb. La prevalencia de vb por el Gram, en las 423 mujeres estudiadas, fue de 20.8% (88 casos y la prevalencia de vb por Papanicolaou, fue del 21 % (89 casos. La sensibilidad fue 87.5%, la especificidad 96.4%, valor predictivo positivo (VPP 86,5% y valor predictivo negativo (VPN 96.7%. La alta sensibilidad, especificidad y valores predictivos de la prueba de Papanicolaou, reportados en este estudio hacen de ésta un buen método de tamizaje, dado que es un examen que se realiza de rutina y no se requiere una prueba adicional para hacer el diagnóstico; lo que a su vez permite disminuir costos porque se omite el estudio de la flora vaginal

  4. Engineering of carbon distribution between glycolysis and sugar nucleobiosynthesis in Lactococcus lactis

    NARCIS (Netherlands)

    Boels, I.C.; Kleerebezem, M.; Vos, de W.M.

    2003-01-01

    We describe the effects of modulating the activities of glucokinase, phosphofructokinase, and phosphoglucomutase on the branching point between sugar degradation and the biosynthesis of sugar nucleotides involved in the production of exopolysaccharide biosynthesis by Lactococcus lactis. This was rea

  5. Physiological Adaptation of the Bacterium Lactococcus lactis in Response to the Production of Human CFTR

    NARCIS (Netherlands)

    Steen, Anton; Wiederhold, Elena; Gandhi, Tejas; Breitling, Rainer; Slotboom, Dirk Jan

    2011-01-01

    Biochemical and biophysical characterization of CFTR (the cystic fibrosis transmembrane conductance regulator) is thwarted by difficulties to obtain sufficient quantities of correctly folded and functional protein. Here we have produced human CFTR in the prokaryotic expression host Lactococcus lacti

  6. Caracterización de tres cepas bacterianas capaces de fijar nitrógeno y biodegradar hidrocarburos aisladas de un suelo de la Patagonia

    Directory of Open Access Journals (Sweden)

    A.J. Acuña

    2010-01-01

    Full Text Available El objetivo de este trabajo fue caracterizar tres microorganismos capaces de biodegradar hidrocarburos en ausencia de nitrógeno aislados a partir de un suelo de la Patagonia con una larga historia de contaminación por petróleo y sus derivados. Para ello se aislaron tres cepas bacterianas de un suelo patagónico con baja concentración de nitrógeno y contaminado con hidrocarburos. Estos fueron identificados por ARNr 16S y ácidos grasos de membrana. También se estudiaron sus perfiles de biodegradación de hidrocarburos en presencia y en ausencia de nitrógeno con hidrocarburos alifáticos, cíclicos, aromáticos y poliaromáticos. La capacidad de realizar fijación biológica de nitrógeno se estudió por reducción de acetileno y detección del gen nifD. Por otro lado, se realizó un estudio por desarrollo en cultivo líquido para conocer la adaptación a diferentes pH, entre 5 y 9, temperatura, de 15 a 65 °C, y concentraciones crecientes de NaCl entre 0 y 3M. Los microorganismos aislados correspondieron a los géneros Pseudomonas, Bacillus y Gordonia, todos con capacidad de fijar nitrógeno. Las cepas biodegradaron casi la totalidad de los hidrocarburos ensayados en presencia y ausencia de nitrógeno y tuvieron una buena adaptación a los cambios de pH, temperatura y concentración de NaCl, demostrando una buena adaptación a los principales factores de estrés en los suelos patagónicos.

  7. Mogućnost primjene bakterije Lactococcus lactis ssp. lactis kao funkcionalne starter kulture

    OpenAIRE

    Frece, Jadranka; Cvrtila, Jelena; Topić, Ivana; Delaš, Frane; Markov, Ksenija

    2014-01-01

    Svrha je ovoga istraživanja bila identificirati i okarakterizirati potencijalne autohtone funkcionalne starter kulture izolirane iz domaće kobasice proizvedene od konjskog mesa. Dominantnu su mikrofloru u uzorcima kobasica činile bakterije mliječne kiseline (BMK), a zatim mikrokoki. Od bakterija mliječne kiseline prevladavale su vrste Lactococcus lactis ssp. lactis i Lactobacillus plantarum. Vrsta Lactococcus lactis ssp. lactis nije uobičajena u fermentiranim kobasicama, pa smo ju okarakteriz...

  8. Fontes de resistência à murcha bacteriana em germoplasma de Capsicum spp. do estado do Amazonas Sources of resistance against bacterial wilt in Capsicum spp. germoplasm of the Amazonas state

    Directory of Open Access Journals (Sweden)

    Liane Cristine Rebouças Demosthenes

    2011-01-01

    Full Text Available A murcha bacteriana, causada por Ralstonia solanacearum, é uma das doenças mais importantes do gênero Capsicum no Brasil. No Amazonas, as condições de elevada temperatura e umidade favorecem o desenvolvimento da doença. O objetivo deste trabalho foi avaliar a resistência à murcha bacteriana de germoplasma, selvagem e comercial, de Capsicum spp. Foram avaliados 22 acessos de Capsicum em casa de vegetação. A inoculação foi feita mediante ferimento das raízes, seguido de adição no solo, ao redor das plantas, de suspensão bacteriana na concentração de 10(8 ufc mL-1. A avaliação foi feita diariamente a partir do quarto dia após a inoculação, em função desenvolvimento dos sintomas. A partir das médias de progresso dos sintomas foi construída a área abaixo da curva de progresso da doença (AACPD, e os dados submetidos ao teste de Scott-Knott ao nível de 5% de probabilidade, utilizando o programa estatístico SAEG 9.1. Foram selecionados os acessos 30, 20 e 17, da espécie C. chinense, como resistentes à murcha bacteriana para ensaios futuros em programas de melhoramento genético.The bacterial wilt caused by Ralstonia solanacearum is one of the most important in the genus Capsicum in Brazil. In the state of Amazonas, high temperatures and humidity favor the development of the disease. The objective of this work was to evaluate resistance in germoplasm of wild and commercial Capsicum spp. to bacterial wilt. Twenty two accesses of Capsicum spp. were evaluated in greenhouse conditions. The inoculation was made by means of wounds in the roots, followed by addition of bacterial suspension in the concentration of 10(8 ufc ml-1 in the soil, around the plants. Plant evaluation was made daily after the fourth day of the inoculation (DAI considering the symptoms progress. From the average progress of symptoms was constructed the area under the disease progress curve (AUDPC, and the data submitted to the Scott-Knott test at 5% of

  9. Epidemiology of bacterial meningitis among children in Brazil, 1997-1998 Epidemiologia de meningites bacterianas entre crianças no Brasil, 1997 a 1998

    Directory of Open Access Journals (Sweden)

    Débora PL Weiss

    2001-06-01

    , atendimento em pronto-socorros, certificados de óbito, autópsias ou atendimentos em ambulatórios. RESULTADOS: A incidência de meningites bacterianas (n=274 foi de 334,9; 115 e 43,5 casos por 10(5 pessoas-ano entre residentes, em Campinas, com idade inferior a 1,5 e 20 anos, respectivamente. Em todos os casos houve hospitalização com uma média de permanência no hospital de 12 dias. O uso prévio de antimicrobianos notificado foi de 4,0%. A taxa de mortalidade total devida a meningites bacterianas entre pessoas com idade inferior a 20 anos foi de 9% (24/274, com 75% das mortes ocorrendo entre crianças com idade inferior a 5 anos. A incidência de meningites causadas por Haemophilus influenzae tipo b (Hib (n=26 foi de 62,8 e 17 casos por 10(5 pessoas-ano em crianças com idade inferior a 1 e 5 anos, respectivamente. CONCLUSÕES: A incidência e a epidemiologia descritiva de meningites causadas por Haemophilus influenzae tipo b (Hib, entre crianças com idade inferior a 5 anos, em Campinas, SP, Brasil, durante o período de 1997 a 1998, foram semelhantes às relatadas pelos Estados Unidos, Europa Ocidental e Israel no período anterior ao extenso uso da vacina contra o Haemophilus influenzae tipo b nessas regiões. O estudo fornece valores de referência para outras pesquisas posteriores que avaliem mudanças na etiologia e na incidência de meningites bacterianas em crianças, depois da introdução de vacinação rotineira contra Haemophilus influenzae tipo b, no Brasil.

  10. Evaluation of tomato rootstocks and its use to control bacterial wilt diseaseAvaliação de porta-enxertos de tomateiro e o uso da enxertia no controle da murcha bacteriana

    Directory of Open Access Journals (Sweden)

    Francisco Ferraz Laranjeira

    2012-05-01

    ção da resistência a R. solanacearum, utilizaram-se quatro genótipos de tomateiro silvestres coletados em diferentes regiões da Bahia, a cv. Santa Clara como testemunha suscetível e o Hawaii 7996 (H7996 como testemunha resistente. Para a avaliação da enxertia no controle da murcha bacteriana do tomateiro, utilizou-se como porta-enxerto o H7996 e como enxerto as cultivares Santa Clara, Santa Cruz Kada e Débora Plus. Os dois experimentos foram avaliados em infectário de R. solanacearum, por um período de 65 dias, para a seleção de porta-enxertos e 45 dias para a avaliação da enxertia, observando-se o sintoma de murcha bacteriana. Apenas o H7996 pode ser recomendado como porta-enxerto resistente a R. solanacearum. Os demais genótipos apresentaram suscetibilidade ao patógeno. A enxertia com esse híbrido não apresentou incompatibilidade com as cultivares avaliadas e promoveu 100 % de controle da murcha bacteriana, em todos os tratamentos, sugerindo que essa técnica pode ser utilizada como método alternativo de controle da murcha bacteriana, permitindo a produção de cultivares comerciais de tomateiro, suscetíveis à doença, em áreas infestadas com R. solanacearum.

  11. Elucidating Flux Regulation of the Fermentation Modes of Lactococcus lactis

    DEFF Research Database (Denmark)

    Chan, Siu Hung Joshua

    The long history of application to the dairy industry has established Lactococcus lactis (L. lactis), the lactic acid bacterium, as one of the most extensively characterized low GC organisms. The relatively simple metabolism of L. lactis has also made it an attractive target for metabolic...... engineering for the production of non-food related chemicals. Moreover, the status of being the first genetically modified organism to deliver immunoproteins alive to human has brought L. lactis considerable fame in biomedical research. Beside the exceptional industrial relevance of L. lactis, it is also an...... amounts of formate, acetate and ethanol are formed, known as mixed-acid fermentation. This shift is termed the mixedacid shift. This type of shift between a low-yield and a high-yield metabolism has drawn a lot of research focus and has similarly been observed in other bacteria, yeast and even tumor cells...

  12. Infective endocarditis with Lactococcus garvieae in Japan: a case report

    Directory of Open Access Journals (Sweden)

    Isonuma Hiroshi

    2011-08-01

    Full Text Available Abstract Introduction Lactococcus garvieae is a well-recognized fish pathogen, and it is considered a rare pathogen with low virulence in human infection. We describe the 11th case of L. garvieae infective endocarditis reported in the literature, and the first reported case in Japan. Case presentation We report a case of a 55-year-old Japanese woman who had native valve endocarditis with L. garvieae. The case was complicated by renal infarction, cerebral infarction, and mycotic aneurysms. After anti-microbial treatment, she was discharged from the hospital and is now well while being monitored in the out-patient clinic. Conclusion We encountered a case of L. garvieae endocarditis that occurred in a native valve of a healthy woman. The 16S ribosomal RNA gene sequencing was useful for the identification of this pathogen. Although infective endocarditis with L. garvieae is uncommon, it is possible to treat high virulence clinically.

  13. Role of intestinal bacterial overgrowth and intestinal motility in bacterial translocation in experimental cirrhosis Papel del sobrecrecimiento bacteriano intestinal y de la motilidad intestinal en la traslocación bacteriana en un modelo experimental de cirrosis

    Directory of Open Access Journals (Sweden)

    E. Sánchez

    2005-11-01

    Full Text Available Background: intestinal bacterial overgrowth (IBO is related to small bowel motility and has been involved in the pathogenesis of bacterial translocation (BT in experimental models, and both overgrowing gut flora and translocating bacteria to mesenteric lymph nodes are common features in cirrhosis. Objectives: the aims of this study were to analyze cecal aerobic bacteria and intestinal transit in cirrhotic rats, and their relationship with BT, evaluating the role of intestinal bacterial overgrowth and small bowel dismotility in the development of BT in experimental cirrhosis. Material and methods: we included twenty-seven male Sprague-Dawley rats with carbon tetrachloride-induced cirrhosis without ascites and ten controls. Cultures of mesenteric lymph nodes (MLN, peripheral and portal blood, liver, spleen and cecal samples were carried out. Small intestinal transit was determined in ten cirrhotic rats and in ten control rats. Results: the prevalence of bacterial translocation was 56%. Total cecal aerobic bacteria count was significantly higher in cirrhotic rats than in control rats (p Introducción: el sobrecrecimiento bacteriano intestinal (SBI está relacionado con la motilidad del intestino delgado y diferentes trabajos con modelos experimentales han sugerido su relación con el desarrollo de traslocación bacteriana (TB. Tanto el sobrecrecimiento bacteriano intestinal como la traslocación bacteriana son eventos frecuentes en la cirrosis hepática. Objetivos: los objetivos de este estudio han sido analizar la población cecal de bacterias aerobias y el tránsito intestinal en un modelo de ratas cirróticas y su relación con la TB. Material y métodos: el estudio se ha realizado en un modelo experimental de cirrosis inducida por tetracloruro de carbono por vía oral en ratas Sprague-Dawley. Se llevaron a cabo cultivos microbiológicos convencionales a partir de ganglios linfáticos mesentéricos (GLM, sangre portal y periférica, h

  14. Resistencia bacteriana a los antibióticos: una crisis global

    OpenAIRE

    Alós Cortés, Juan Ignacio

    2015-01-01

    La introducción de los antibióticos en la práctica clínica supuso una de las intervenciones más importantes para el control de las enfermedades infecciosas. Los antibióticos han salvado millones de vidas, y además han supuesto una revolución en la medicina. Sin embargo, una amenaza creciente deteriora la eficacia de estos fármacos: la resistencia bacteriana a los antibióticos, que se define en este trabajo como la capacidad de una bacteria para sobrevivir en concentraciones de antibiótico que...

  15. Tratamento da vaginose bacteriana com gel vaginal de Aroeira (Schinus terebinthifolius Raddi): ensaio clínico randomizado

    OpenAIRE

    Amorim Melania Maria Ramos de; Santos Luiz Carlos

    2003-01-01

    OBJETIVOS: testar a eficácia e a tolerância do gel de aroeira (Schinus terebinthifolius Raddi) para tratamento da vaginose bacteriana. MÉTODOS: quarenta e oito mulheres com vaginose bacteriana sintomática (de acordo com os critérios de Amsel) foram incluídas em ensaio clínico randomizado, duplo-cego, controlado, comparando-se o uso do gel vaginal de aroeira (25 casos) com placebo (23 casos). Os principais desfechos avaliados foram: taxa de cura, presença de lactobacilos na colpocitologia depo...

  16. Bacteriemia por Ochrobactrum anthropi en paciente con obstrucción de la vía biliar Bacteraemia by Ochrobactrum anthropi in patient with biliary obstruction

    OpenAIRE

    Sigifredo Ospina; Santiago Atehortúa Muñoz; Juan Zapata

    2009-01-01

    Reporte de caso de bacteriemia por Ochrobactrum anthropi con probable hepatitis bacteriana asociada, en un paciente inmunocompetente, el cual ingresa por sospecha de colangitis y obstrucción biliar. O. anthropi es una bacteria emergente en infecciones intrahospitalarias con notable resistencia antimicrobiana, y es un patógeno inusual en humanos.A case report of bacteraemia by Ochrobactrum anthropi probably associated with bacterial hepatitis, in a inmuno competent patient, who was admitted to...

  17. Endoftalmites bacterianas com culturas positivas: uma revisão de 6 anos Culture proven bacterial endophthalmitis: a 6-year review

    OpenAIRE

    Paulo José Martins Bispo; Gustavo Barreto Melo; Pedro Alves d'Azevedo; Ana Luisa Höfling-Lima; Maria Cecília Zorat Yu; Antonio Carlos Campos Pignatari

    2008-01-01

    OBJETIVO: Determinar a distribuição dos microrganismos isolados de pacientes com endoftalmite bacteriana e sua sensibilidade a antimicrobianos. MÉTODOS: Foram analisados retrospectivamente os dados clínicos e microbiológicos dos pacientes com hipótese diagnóstica de endoftalmite e cultura bacteriana positiva, atendidos no Departamento de Oftalmologia da UNIFESP de 1º de janeiro de 2000 a 31 de dezembro de 2005. RESULTADOS: De 451 pacientes, 153 (33,9%) apresentaram cultura bacteriana positiva...

  18. Heterologous Expression of the Pneumococcal Serotype 14 Polysaccharide in Lactococcus lactis Requires Lactococcal epsABC Regulatory Genes▿ †

    OpenAIRE

    Nierop Groot, M.N.; Godefrooij, J.; Kleerebezem, M.

    2007-01-01

    The pneumococcal serotype 14 polysaccharide was produced in Lactococcus lactis by coexpressing pneumococcal polysaccharide type 14-specific genes (cpsFGHIJKL(14)) with the lactococcal regulatory and priming glucosyltransferase-encoding genes specific for B40 polysaccharide (epsABCD(B40)). The polysaccharide produced by Lactococcus was secreted in the medium, simplifying downstream processing and polysaccharide isolation from culture broth

  19. Evaluación de la eficacia de dos prescripciones de cepillos dentales en la remoción de placa bacteriana en pacientes ortodóncicos Evaluation of two toothbrushes prescriptions on the removal of plaque in patients with fixed orthodontic appliances

    OpenAIRE

    D Zúñiga García; EJ Pastén Castro; PA Araya-Díaz; H Palomino Montenegro

    2012-01-01

    La aparatología empleada en los tratamientos de ortodoncia, favorece la retención de placa bacteriana y dificulta su eliminación por parte del paciente, aumentando el riesgo de desarrollar caries, manchas blancas y enfermedad periodontal. Diferentes cepillos dentales se han desarrollado para facilitar la mantención de una adecuada higiene oral en estos pacientes, sin embargo, se ha observado que los cepillos comunmente indicados (cepillo ortodóncico mas cepillo unipenacho), no siempre son bie...

  20. Ceftazidima en infusión continua en infecciones por Pseudomona aeruginosa

    OpenAIRE

    Alexis Culay Pérez; Ramón Ferriol Torres; Carlos Miguel Sarduy Ramos; Galys Cervantes Núñez

    2012-01-01

    Fundamento: la ceftazidima es un antimicrobiano perteneciente a la familia de las cefalosporinas de la tercera generación, está indicada en el tratamiento de infecciones bacterianas graves, simples o mixtas, su administración en infusión continua permite optimizar la concentración del antibiótico al mantenerse por encima de su concentración mínima inhibitoria. Objetivo: evaluar el uso de la ceftazidima en infusión continua en infecciones intrahospitalarias por Pseudomona aeruginosa. Método: s...

  1. Some chemical properties of nisin produced by lactococcus lactis

    International Nuclear Information System (INIS)

    The present study was carried out to study the properties of nisin produced by lactococcus lactis FG2 isolated from local un fated cheese. The maximum anti-microbial effect of pure nisin was occurred at ph 6 and 7. Nisin was heat stable from 40 to 90 degree C for 30 min. Molecular weight of nisin was determined by SDS-PAGE, it was 3.0 kDa and after irradiated the microbial cells to 1.5 kGy dose level the molecular weight increased to 3.5 t kDa then decreased at 2 kGy . Storage for two weeks it appeared in dimmer means and had a molecular weight 7 kDa . Using amino acid analyzer reveled that nisin contained a majority of nonpolar amino acids and exhibited cystine in composition . Nisin produced in whey have higher activity than nisin produced in MRS medium but both had the same structure. The results proved that nisin gene is in coded in chromosome and not with plasmid.

  2. Functional Expression of an Orchid Fragrance Gene in Lactococcus lactis

    Directory of Open Access Journals (Sweden)

    Adelene Ai Lian Song

    2012-02-01

    Full Text Available Vanda Mimi Palmer (VMP, an orchid hybrid of Vanda tesselata and Vanda Tan Chay Yan is a highly scented tropical orchid which blooms all year round. Previous studies revealed that VMP produces a variety of isoprenoid volatiles during daylight. Isoprenoids are well known to contribute significantly to the scent of most fragrant plants. They are a large group of secondary metabolites which may possess valuable characteristics such as flavor, fragrance and toxicity and are produced via two pathways, the mevalonate (MVA pathway or/and the 2-C-methyl-D-erythritol-4-phosphate (MEP pathway. In this study, a sesquiterpene synthase gene denoted VMPSTS, previously isolated from a floral cDNA library of VMP was cloned and expressed in Lactococcus lactis to characterize the functionality of the protein. L. lactis, a food grade bacterium which utilizes the mevalonate pathway for isoprenoid production was found to be a suitable host for the characterization of plant terpene synthases. Through recombinant expression of VMPSTS, it was revealed that VMPSTS produced multiple sesquiterpenes and germacrene D dominates its profile.

  3. Genetic investigation within Lactococcus garvieae revealed two genomic lineages.

    Science.gov (United States)

    Ferrario, Chiara; Ricci, Giovanni; Borgo, Francesca; Rollando, Alessandro; Fortina, Maria Grazia

    2012-07-01

    The diversity of a collection of 49 Lactococcus garvieae strains, including isolates of dairy, fish, meat, vegetable and cereal origin, was explored using a molecular polyphasic approach comprising PCR-ribotyping, REP and RAPD-PCR analyses and a multilocus restriction typing (MLRT) carried out on six partial genes (atpA, tuf, dltA, als, gapC, and galP). This approach allowed high-resolution cluster analysis in which two major groups were distinguishable: one group included dairy isolates, the other group meat isolates. Unexpectedly, of the 12 strains coming from fish, four grouped with dairy isolates, whereas the others with meat isolates. Likewise, strains isolated from vegetables allocated between the two main groups. These findings revealed high variability within the species at both gene and genome levels. The observed genetic heterogeneity among L. garvieae strains was not entirely coherent with the ecological niche of origin of the strains, but rather supports the idea of an early separation of L. garvieae population into two independent genomic lineages. PMID:22568590

  4. Estomatitis ulcerativa infecciosa como consecuencia de un traumatismo en una Boa constrictor con desarrollo de neumonía bacteriana y septicemia.

    Directory of Open Access Journals (Sweden)

    Zulma Esperanza Rojas-Sereno

    2015-12-01

    Full Text Available Las neumonías bacterianas se asocian con estomatitis en serpientes, debido al descenso de los exudados de la cavidad hacia el pulmón. El objetivo de este reporte es describir los hallazgos clínicos, y el manejo diagnóstico y terapéutico de una Boa constrictor con estomatitis ulcerativa infecciosa (EUI postraumática que se complicó con neumonía bacteriana (NB y septicemia (SE. Un adulto de B. constrictor, ingresó con mucosas congestionadas, presencia de tierra en la cavidad oral, ulceraciones gingivales, deshidratación marcada y tegumento reseco con retención de muda. Presentó además letargia con postura anómala de la cabeza, con leve aumento de tamaño a nivel dorsal y antecedente de trauma. Se observó anemia no regenerativa y leve monocitosis relativa. Los hallazgos radiográficos fueron compatibles con fractura parietal e inflamación del tejido blando circundante. Se estabilizó el paciente y se realizó lavado bucal y debridación, posteriormente se administró tratamiento antibiótico y analgésico. La evolución no fue satisfactoria y terminó con la muerte del animal. El diagnóstico final fue una severa neumonía supurativa necrotizante de origen bacteriano posiblemente por Pasteurella o Pseudomona, con SE secundaria. El cuadro clínico fue típico de EUI de tipo crónico, con desarrollo de una NB y un cuadro septicémico, generando una tríada de patologías que hizo que el pronóstico del paciente fuera malo pese al continuo tratamiento. Es necesaria la determinación microbiológica del agente para mejorar la eficacia de la terapia antibiótica, aunque muchas de las bacterias de ofidios son resistentes a la antibioterapia.

  5. Assessing the diversity of bacterial communities associated with plants Avaliação da diversidade de comunidades bacterianas associadas às plantas

    Directory of Open Access Journals (Sweden)

    Fernando Dini Andreote

    2009-09-01

    Full Text Available Plant-bacteria interactions result from reciprocal recognition between both species. These interactions are responsible for essential biological processes in plant development and health status. Here, we present a review of the methodologies applied to investigate shifts in bacterial communities associated with plants. A description of techniques is made from initial isolations to culture-independent approaches focusing on quantitative Polymerase Chain Reaction in real time (qPCR, Denaturing Gradient Gel Electrophoresis (DGGE, clone library construction and analysis, the application of multivariate analyses to microbial ecology data and the upcoming high throughput methodologies such as microarrays and pyrosequencing. This review supplies information about the development of traditional methods and a general overview about the new insights into bacterial communities associated with plants.As interações planta-bactéria resultam de um reconhecimento recíproco de ambas espécies. Estas interações são responsáveis por processos biológicos essenciais para o desenvolvimento e a proteção das plantas. Este trabalho revisa as metodologias aplicadas na investigação de alterações nas comunidades bacterianas associadas às plantas. Uma descrição das técnicas é feita, desde o isolamento até a aplicação de técnicas independentes de cultivo, destacando as técnicas de qPCR, Gel de Eletroforese em Gradiente Desnaturante (DGGE, construção e análise de bibliotecas de clones, a aplicação de análise multivariada em dados de ecologia microbiana, e as novas metodologias de alto processamento de amostras como microarranjos e pirosequenciamento. Em resumo, esta revisão fornece informações sobre o desenvolvimento das técnicas tradicionais e uma visão geral sobre as novas tendências dos estudos de comunidades bacterianas associadas às plantas.

  6. Infeções hospitalares bacterianas no século XXI

    OpenAIRE

    Simões, Cristina Maria Senra Barroso

    2016-01-01

    No decorrer do século XX, a descoberta da penicilina veio contribuir para o controlo das infeções bacterianas, entre elas as hospitalares. Contudo, em pleno século XXI, apesar do avanço tecnológico e científico, as infeções hospitalares estão associadas a altas taxas de morbilidade e mortalidade em todo o mundo. As infeções hospitalares apresentam-se como um problema transversal às várias instituições, e muito difícil de resolver. Nos últimos anos as infeções hospitalares têm registado um aum...

  7. Genome Sequence of Lactococcus lactis subsp. lactis NCDO 2118, a GABA-Producing Strain

    DEFF Research Database (Denmark)

    Oliveira, Letícia C; Saraiva, Tessália D L; Soares, Siomar C;

    2014-01-01

    Lactococcus lactis subsp. lactis NCDO 2118 is a nondairy lactic acid bacterium, a xylose fermenter, and a gamma-aminobutyric acid (GABA) producer isolated from frozen peas. Here, we report the complete genome sequence of L. lactis NCDO 2118, a strain with probiotic potential activity.......Lactococcus lactis subsp. lactis NCDO 2118 is a nondairy lactic acid bacterium, a xylose fermenter, and a gamma-aminobutyric acid (GABA) producer isolated from frozen peas. Here, we report the complete genome sequence of L. lactis NCDO 2118, a strain with probiotic potential activity....

  8. Resistance to antibiotics in Lacid acid bacteria - strain Lactococcus

    Directory of Open Access Journals (Sweden)

    Filipić Brankica

    2015-01-01

    Full Text Available Lactic acid bacteria (LAB are widely used in the food industry, especially in the production of fermented dairy products and meat. The most studied species among Lis Lactococcus lactis. L. lactis strains are of great importance in the production of fermented dairy products such as yogurt, butter, fresh cheese and some kind of semi-hard cheese. Although L. lactis acquired the „Generally Regarded As Safe“ (GRAS status, many investigations indicated that lactococci may act as reservoirs of antibiotic resistance genes, which could be transferred to other bacterial species in human gastrointestinal tract includ­ing pathogens. The genome analysis of L. lactis indicated the presence of at least 40 putative drug transporter genes, and only four multidrug resistance (MDR transporters are functionally characterized: LmrA, LmrP, LmrCD i CmbT. LmrA is the first described MDR transporter in prokaryotes. LmrCD is responsible for resistance to cholate, which is an integral part of human bile and LmrCD is important for intestinal survival of lactococci that are used as probiotics. Secondary multidrug transporter LmrP confers resistance to lincosamides, macrolides, streptogramins and tetracyclines. CmbT protein has an effect on the host cell resistance to lincomycin, sulfadiazine, streptomycin, rifampicin, puromycin and sulfametox­azole. Since the food chain is an important way of transmitting resistance genes in human and animal population, it is of great importance to study the mechanisms of resistance in lactococci and other LAB, intended for the food industry. [Projekat Ministarstva nauke Republike Srbije, br. 173019: Izučavanje gena i molekularnih mehanizama u osnovi probiotičke aktivnosti bakterija mlečne kiseline izolovanih sa područja Zapadnog Balkana

  9. Modeling Lactococcus lactis using a genome-scale flux model

    Directory of Open Access Journals (Sweden)

    Nielsen Jens

    2005-06-01

    Full Text Available Abstract Background Genome-scale flux models are useful tools to represent and analyze microbial metabolism. In this work we reconstructed the metabolic network of the lactic acid bacteria Lactococcus lactis and developed a genome-scale flux model able to simulate and analyze network capabilities and whole-cell function under aerobic and anaerobic continuous cultures. Flux balance analysis (FBA and minimization of metabolic adjustment (MOMA were used as modeling frameworks. Results The metabolic network was reconstructed using the annotated genome sequence from L. lactis ssp. lactis IL1403 together with physiological and biochemical information. The established network comprised a total of 621 reactions and 509 metabolites, representing the overall metabolism of L. lactis. Experimental data reported in the literature was used to fit the model to phenotypic observations. Regulatory constraints had to be included to simulate certain metabolic features, such as the shift from homo to heterolactic fermentation. A minimal medium for in silico growth was identified, indicating the requirement of four amino acids in addition to a sugar. Remarkably, de novo biosynthesis of four other amino acids was observed even when all amino acids were supplied, which is in good agreement with experimental observations. Additionally, enhanced metabolic engineering strategies for improved diacetyl producing strains were designed. Conclusion The L. lactis metabolic network can now be used for a better understanding of lactococcal metabolic capabilities and potential, for the design of enhanced metabolic engineering strategies and for integration with other types of 'omic' data, to assist in finding new information on cellular organization and function.

  10. Genes but not genomes reveal bacterial domestication of Lactococcus lactis.

    Directory of Open Access Journals (Sweden)

    Delphine Passerini

    Full Text Available BACKGROUND: The population structure and diversity of Lactococcus lactis subsp. lactis, a major industrial bacterium involved in milk fermentation, was determined at both gene and genome level. Seventy-six lactococcal isolates of various origins were studied by different genotyping methods and thirty-six strains displaying unique macrorestriction fingerprints were analyzed by a new multilocus sequence typing (MLST scheme. This gene-based analysis was compared to genomic characteristics determined by pulsed-field gel electrophoresis (PFGE. METHODOLOGY/PRINCIPAL FINDINGS: The MLST analysis revealed that L. lactis subsp. lactis is essentially clonal with infrequent intra- and intergenic recombination; also, despite its taxonomical classification as a subspecies, it displays a genetic diversity as substantial as that within several other bacterial species. Genome-based analysis revealed a genome size variability of 20%, a value typical of bacteria inhabiting different ecological niches, and that suggests a large pan-genome for this subspecies. However, the genomic characteristics (macrorestriction pattern, genome or chromosome size, plasmid content did not correlate to the MLST-based phylogeny, with strains from the same sequence type (ST differing by up to 230 kb in genome size. CONCLUSION/SIGNIFICANCE: The gene-based phylogeny was not fully consistent with the traditional classification into dairy and non-dairy strains but supported a new classification based on ecological separation between "environmental" strains, the main contributors to the genetic diversity within the subspecies, and "domesticated" strains, subject to recent genetic bottlenecks. Comparison between gene- and genome-based analyses revealed little relationship between core and dispensable genome phylogenies, indicating that clonal diversification and phenotypic variability of the "domesticated" strains essentially arose through substantial genomic flux within the dispensable

  11. Mobile CRISPR/Cas-mediated bacteriophage resistance in Lactococcus lactis.

    Directory of Open Access Journals (Sweden)

    Anne M Millen

    Full Text Available Lactococcus lactis is a biotechnological workhorse for food fermentations and potentially therapeutic products and is therefore widely consumed by humans. It is predominantly used as a starter microbe for fermented dairy products, and specialized strains have adapted from a plant environment through reductive evolution and horizontal gene transfer as evidenced by the association of adventitious traits with mobile elements. Specifically, L. lactis has armed itself with a myriad of plasmid-encoded bacteriophage defensive systems to protect against viral predation. This known arsenal had not included CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins, which forms a remarkable microbial immunity system against invading DNA. Although CRISPR/Cas systems are common in the genomes of closely related lactic acid bacteria (LAB, none was identified within the eight published lactococcal genomes. Furthermore, a PCR-based search of the common LAB CRISPR/Cas systems (Types I and II in 383 industrial L. lactis strains proved unsuccessful. Here we describe a novel, Type III, self-transmissible, plasmid-encoded, phage-interfering CRISPR/Cas discovered in L. lactis. The native CRISPR spacers confer resistance based on sequence identity to corresponding lactococcal phage. The interference is directed at phages problematic to the dairy industry, indicative of a responsive system. Moreover, targeting could be modified by engineering the spacer content. The 62.8-kb plasmid was shown to be conjugally transferrable to various strains. Its mobility should facilitate dissemination within microbial communities and provide a readily applicable system to naturally introduce CRISPR/Cas to industrially relevant strains for enhanced phage resistance and prevention against acquisition of undesirable genes.

  12. Candida albicans skin abscess Abscesso de pele por Candida albicans

    Directory of Open Access Journals (Sweden)

    Felipe Francisco Tuon

    2006-10-01

    Full Text Available Subcutaneous candidal abscess is a very rare infection even in immunocompromised patients. Some cases are reported when breakdown in the skin occurs, as bacterial cellulites or abscess, iatrogenic procedures, trauma and parenteral substance abuse. We describe a case of Candida albicans subcutaneous abscess without fungemia, which can be associated with central venous catheter.Abscesso subcutâneo por Candida é infecção muito rara mesmo em pacientes imunocomprometidos. Alguns casos são relatados quando ocorre dano na pele, como celulite bacteriana ou abscesso, procedimentos iatrogênicos, trauma e abuso de substância parenteral. Relatamos caso de abscesso subcutâneo por Candida albicans sem fungemia, que pode estar associado com cateter venoso central.

  13. Cuerpos de inclusión, células bacterianas y composiciones que los contienen y sus usos

    OpenAIRE

    Villaverde Corrales, Antonio Pedro; Vázquez Gómez, Esther; Díez Gil, César; García Fruitós, Elena; Ratera Bastardas, Inmaculada; Veciana Miró, Jaume

    2008-01-01

    Cuerpos de inclusión, células bacterianas y composiciones que los contienen y sus usos. La presente invención se refiere a un cuerpo de inclusión aislado que comprende un polipéptido caracterizado porque el cuerpo da inclusión está en forma particulada. La presente invención también se refiere a una célula bacteriana que comprenda dicho cuerpo de inclusión. La presente invención se refiere además a una composición que comprende dicho cuerpo de inclusión y una célula eu...

  14. Tratamento da vaginose bacteriana com gel vaginal de Aroeira (Schinus terebinthifolius Raddi: ensaio clínico randomizado

    Directory of Open Access Journals (Sweden)

    Amorim Melania Maria Ramos de

    2003-01-01

    Full Text Available OBJETIVOS: testar a eficácia e a tolerância do gel de aroeira (Schinus terebinthifolius Raddi para tratamento da vaginose bacteriana. MÉTODOS: quarenta e oito mulheres com vaginose bacteriana sintomática (de acordo com os critérios de Amsel foram incluídas em ensaio clínico randomizado, duplo-cego, controlado, comparando-se o uso do gel vaginal de aroeira (25 casos com placebo (23 casos. Os principais desfechos avaliados foram: taxa de cura, presença de lactobacilos na colpocitologia depois do tratamento e efeitos colaterais. Realizou-se análise estatística usando os testes chi2 e exato de Fisher, ao nível de significância de 5%. RESULTADOS: adotando-se os parâmetros clínicos de Amsel para vaginose bacteriana, a taxa de cura foi de 84% no grupo da aroeira e 47,8% no grupo placebo (p = 0,008. Observou-se freqüência significativamente maior de lactobacilos na colpocitologia entre as pacientes tratadas com aroeira (43,5% em relação ao placebo (4,3% (p = 0,002. Efeitos adversos relacionados ao tratamento não foram freqüentes em ambos os grupos. CONCLUSÕES: o presente estudo indica que o gel vaginal de aroeira é efetivo e seguro para o tratamento da vaginose bacteriana. Além disso, sugerem-se potenciais efeitos benéficos na flora vaginal.

  15. The structure of the lantibiotic lacticin 481 produced by Lactococcus lactis : location of the thioether bridges

    NARCIS (Netherlands)

    Hooven, Henno W. van den; Lagerwerf, Fija M.; Heerma, Wigger; Haverkamp, Johan; Piard, Jean-Christophe; Hilbers, Cornelis W.; Siezen, Roland J.; Kuipers, Oscar P.; Rollema, Harry S.

    1996-01-01

    The lantibiotic lacticin 481 is a bacteriocin produced by Lactococcus lactis ssp. lactis. This polypeptide contains 27 amino acids, including the unusual residues dehydrobutyrine and the thioether-bridging lanthionine and 3-methyllanthionine. Lacticin 481 belongs to a structurally distinct group of

  16. Lactococcus lactis Dihydroorotate Dehydrogenase A Mutants Reveal Important Facets of the Enzymatic Function

    DEFF Research Database (Denmark)

    Nørager, Sofie Charlotte; Arent, S; Björnberg, Olof;

    2003-01-01

    and 1B, and class 2. This division corresponds to differences in cellular location and the nature of the electron acceptor. Herein we report a study of Lactococcus lactis DHODA, a representative of the class 1A enzymes. Based on the DHODA structure we selected seven residues that are highly conserved...

  17. 21 CFR 184.1985 - Aminopeptidase enzyme preparation derived from lactococcus lactis.

    Science.gov (United States)

    2010-04-01

    ...) and 1 CFR part 51. Copies are available from the National Academy Press, 2101 Constitution Ave. NW... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Aminopeptidase enzyme preparation derived from... Aminopeptidase enzyme preparation derived from lactococcus lactis. (a) Aminopeptidase enzyme preparation...

  18. Development, molecular characterization and exploitation of the nisin controlled expression system in Lactococcus lactis.

    NARCIS (Netherlands)

    Ruyter, de P.G.G.A.

    1998-01-01

    Lactic acid bacteria are gram-positive bacteria that are widely used in a variety of dairy fermentation processes. Notably, strains of the lactic acid starter bacterium Lactococcus lactis are of great economic importance because of their world-wide use in cheese making. The characteristic aroma, fla

  19. Complete Genome Sequence of Nonagglutinating Lactococcus garvieae Strain 122061 Isolated from Yellowtail in Japan

    Science.gov (United States)

    Nishiki, Issei; Oinaka, Daisaku; Iwasaki, Yuki; Yasuike, Motoshige; Nakamura, Yoji; Yoshida, Terutoyo; Nagai, Satoshi; Katoh, Masaya; Kobayashi, Takanori

    2016-01-01

    Nonagglutinating Lactococcus garvieae has been isolated from diseased farmed yellowtail in Japan since 2012. In this study, the complete genome and plasmid sequence of nonagglutinating L. garvieae strain 122061 was determined, to our knowledge, for the first time. PMID:27389264

  20. Increasing acidification of nonreplicating Lactococcus lactis Delta thyA mutants by incorporating ATPase activity

    DEFF Research Database (Denmark)

    Pedersen, Martin Bastian; Købmann, Brian Jensen; Jensen, Peter Ruhdal;

    2002-01-01

    Lactococcus lactis MBP71 DeltathyA (thymidylate synthase) cannot synthesize dTTP de novo, and DNA replication is dependent on thymidine in the growth medium. In the nonreplicating state acidification by MBP71 was completely insensitive to bacteriophages (M. B. Pedersen, P. R. Jensen, T. Janzen, and...

  1. Sec-Mediated Transport of Posttranslationally Dehydrated Peptides in Lactococcus lactis

    NARCIS (Netherlands)

    Kuipers, Anneke; Wierenga, Jenny; Rink, Rick; Kluskens, Leon D.; Driessen, Arnold J.M.; Kuipers, Oscar P.; Moll, Gert N.

    2006-01-01

    Nisin is a lanthionine-containing antimicrobial peptide produced by Lactococcus lactis. Its (methyl)lanthionines are introduced by two posttranslational enzymatic steps involving the dehydratase NisB, which dehydrates serine and threonine residues, and the cyclase NisC, which couples these dehydrate

  2. CONTINUOUS MEASUREMENT OF THE CYTOPLASMIC PH IN LACTOCOCCUS-LACTIS WITH A FLUORESCENT PH INDICATOR

    NARCIS (Netherlands)

    MOLENAAR, D; ABEE, T; KONINGS, WN

    1991-01-01

    The cytoplasmic pH of Lactococcus lactis was studied with the fluorescent pH indicator 2',7'-bis-(2-carboxyethyl)-5 (and-6)-carboxyfluorescein (BCECF). A novel method was applied for loading bacterial cells with BCECF, which consists of briefly treating a dense cell suspension with acid in the prese

  3. Controlles modulation of folate polyglutamyl tail length by metabolic engineering of Lactococcus lactis

    NARCIS (Netherlands)

    Sybesma, W.F.H.; Born, van den E.; Starrenburg, M.; Mierau, I.; Kleerebezem, M.; Vos, de W.M.; Hugenholtz, J.

    2003-01-01

    The dairy starter bacterium Lactococcus lactis is able to synthesize folate and accumulates >90% of the produced folate intracellularly, predominantly in the polyglutamyl form. Approximately 10% of the produced folate is released into the environment. Overexpression of folC in L. lactis led to an

  4. Quantitative physiology of Lactococcus lactis at extreme low-growth rates

    NARCIS (Netherlands)

    Ercan, O.; Smid, E.J.; Kleerebezem, M.

    2013-01-01

    This paper describes the metabolic adaptation of Lactococcus lactis during the transition from a growing to a non-growing state using retentostat cultivation. Under retentostat cultivation, the specific growth rate decreased from 0.025 h-1 to 0.0001 h-1 in 42 days, while doubling time increased to m

  5. Effect of X-Prolyl Dipeptidyl Aminopeptidase Deficiency on Lactococcus lactis

    NARCIS (Netherlands)

    Mayo, Baltasar; Kok, Jan; Bockelmann, Wilhelm; Haandrikman, Alfred; Leenhouts, Kees J.; Venema, Gerhardus

    1993-01-01

    The genetic determinant (pepXP) of an X-prolyl dipeptidyl aminopeptidase (PepXP) has recently been cloned and sequenced from both Lactococcus lactis subsp. cremoris (B. Mayo, J. Kok, K. Venema, W. Bockelmann, M. Teuber, H. Reinke, and G. Venema, Appl. Environ. Microbiol. 57:38-44, 1991) and L. lacti

  6. Lactococcus lactis YfiA is necessary and sufficient for ribosome dimerization

    NARCIS (Netherlands)

    Puri, Pranav; Eckhardt, Thomas H; Franken, Linda E; Fusetti, Fabrizia; Stuart, Marc C A; Boekema, Egbert J; Kuipers, Oscar P; Kok, Jan; Poolman, Berend

    2014-01-01

    Dimerization and inactivation of ribosomes in Escherichia coli is a two-step process that involves the binding of ribosome modulation factor (RMF) and hibernation promotion factor (HPF). Lactococcus lactisMG1363 expresses a protein, YfiA(Ll), which associates with ribosomes in the stationary phase o

  7. Mechanism of Citrate Metabolism by an Oxaloacetate Decarboxylase-Deficient Mutant of Lactococcus lactis IL1403

    NARCIS (Netherlands)

    Pudlik, Agata M.; Lolkema, Juke S.

    2011-01-01

    Citrate metabolism in resting cells of Lactococcus lactis IL1403(pFL3) results in the formation of two end products from the intermediate pyruvate, acetoin and acetate (A. M. Pudlik and J. S. Lolkema, J. Bacteriol. 193:706-714, 2011). Pyruvate is formed from citrate following uptake by the transport

  8. Relationships between MDR proteins, bacteriocin production and proteolysis in Lactococcus lactis

    NARCIS (Netherlands)

    Gajic, Olivera

    2003-01-01

    The Gram-positive lactic acid bacterium Lactococcus lactis can harbour a wide variety of circular extrachromosomal DNA molecules, so-called plasmids. Many of the traits that make them useful for manufacturing of fermented food products (e.g. bacteriophage resistance, bacteriocin and proteinase produ

  9. Increasing the heme-dependent respiratory efficiency of Lactococcus lactis by inhibition of lactate dehydrogenase.

    Science.gov (United States)

    Arioli, Stefania; Zambelli, Daniele; Guglielmetti, Simone; De Noni, Ivano; Pedersen, Martin B; Pedersen, Per Dedenroth; Dal Bello, Fabio; Mora, Diego

    2013-01-01

    The discovery of heme-induced respiration in Lactococcus lactis has radically improved the industrial processes used for the biomass production of this species. Here, we show that inhibition of the lactate dehydrogenase activity of L. lactis during growth under respiration-permissive conditions can stimulate aerobic respiration, thereby increasing not only growth efficiency but also the robustness of this organism. PMID:23064338

  10. An ABC-type multidrug transporter of Lactococcus lactis possesses an exceptionally broad substrate specificity

    NARCIS (Netherlands)

    Poelarends, GJ; Mazurkiewicz, P; Putman, M; Cool, RH; van Veen, HW; Konings, WN

    2000-01-01

    LmrA is a 590-amino acid membrane protein which confers multidrug resistance on Lactococcus lactis cells by extruding amphiphilic compounds from the inner leaflet of the cytoplasmic membrane at the expense of ATP hydrolysis. Its structural and functional characteristics place it in the P-glycoprotei

  11. Guías de manejo de las queratitis infecciosas bacterianas. Hospital Universitario del Valle, enero-julio 2002 .

    Directory of Open Access Journals (Sweden)

    Alejandro de La Torre

    2009-11-01

    Full Text Available Propósito. Evaluar los resultados de la aplicación de las guías de manejo de las queratitis infecciosas bacterianas en pacientes que llegan al Servicio de Oftalmología. Métodos. Ingresaron con diagnóstico de queratitis infecciosas bacterianas 21 pacientes durante 6 meses. Resultados. De 18 queratitis infecciosas, 16 (88.9% se manejaron medicamente y 2 se les realizó tratamiento quirúrgico; 50% (9 se diagnosticaron como severas, 14 cumplieron criterios para tomar muestras de laboratorio: sensibilidad del Gram es de 50% y la sensibilidad de cultivos para bacterias es de 64.2%. La bacteria encontrada con más frecuencia fue el Staphylococcus epidermidis. Conclusión. El estudio demostró evidentemente que las guías de manejo propuestas para las queratitis infecciosas bacterianas son útiles para lograr su recuperación sin complicaciones (prueba exacta de Fisher p= 0.001032.

  12. Construction and Expression of β-galactosidase Genetically Engineered Lactococcus lactis

    Institute of Scientific and Technical Information of China (English)

    吕晓英; 张朝武; 裴晓方; 刘祥; 余倩; 刘衡川

    2004-01-01

    Our objective is to solve the lactose malabsorption and intolerance of human beings by combining mlcro-ecology path with genetic engineering technique. Plasmid pMG36e was used to clone and express a β-galactosidase gene from L.delbrueckii bulgaricus strain 1. 1480 in the Lactococcus lactis subsp, cremoris MG1363 and Lactococcus lactis subsp. lactis IL1403. The recombinant plasmid was preserved and proliferated in Escherichia coli ( E. coli) JM109, and transformed into MG1363 and 1L1403 by electroporation. The protein expression was studied. (1) The bifidobacterium culture medium (BBL) was suitable for the growth of the strain 1. 1480. (2) With 13 amino acids at the N-terminus from the vector, β-galactosidase fusion protein (which retained the enzyme activity) could be successfully expressed in E. coli JM109, MG1363 and IL1403, but the expression quantity was larger in the former than in the latter two. (3) The SD sequence designed could be successfully recognized by both the E. coli and the Lactococcus lactis, but the expression level of the non-fusion β-galac-tosidase protein was lower than that of the fusion protein in the same host. The β-galactosidase genetically engineered E.coli JM109 is a useful tool to produce this enzyme in vitro. The signal peptide of the usp45 protein from the Lactococcus lactis can be added before the promoter sequence to promote β-galactosidase secretion from Lactococcus lactis. The potential application of the β-galactosidase genetically engineered MG1363 and IL1403 to cure the lactose malabsorption and lactose intolerance in both health food and medicine is promising。

  13. Cell Wall Anchoring of the Campylobacter Antigens to Lactococcus lactis.

    Science.gov (United States)

    Kobierecka, Patrycja A; Olech, Barbara; Książek, Monika; Derlatka, Katarzyna; Adamska, Iwona; Majewski, Paweł M; Jagusztyn-Krynicka, Elżbieta K; Wyszyńska, Agnieszka K

    2016-01-01

    Campylobacter jejuni is the most frequent cause of human food-borne gastroenteritis and chicken meat is the main source of infection. Recent studies showed that broiler chicken immunization against Campylobacter should be the most efficient way to lower the number of human infections by this pathogen. Induction of the mucosal immune system after oral antigen administration should provide protective immunity to chickens. In this work we tested the usefulness of Lactococcus lactis, the most extensively studied lactic acid bacterium, as a delivery vector for Campylobacter antigens. First we constructed hybrid protein - CjaA antigen presenting CjaD peptide epitopes on its surface. We showed that specific rabbit anti-rCjaAD serum reacted strongly with both CjaA and CjaD produced by a wild type C. jejuni strain. Next, rCjaAD and CjaA were fused to the C-terminus of the L. lactis YndF containing the LPTXG motif. The genes expressing these proteins were transcribed under control of the L. lactis Usp45 promoter and their products contain the Usp45 signal sequences. This strategy ensures a cell surface location of both analyzed proteins, which was confirmed by immunofluorescence assay. In order to evaluate the impact of antigen location on vaccine prototype efficacy, a L. lactis strain producing cytoplasm-located rCjaAD was also generated. Animal experiments showed a decrease of Campylobacter cecal load in vaccinated birds as compared with the control group and showed that the L. lactis harboring the surface-exposed rCjaAD antigen afforded greater protection than the L. lactis producing cytoplasm-located rCjaAD. To the best of our knowledge, this is the first attempt to employ Lactic Acid Bacteria (LAB) strains as a mucosal delivery vehicle for chicken immunization. Although the observed reduction of chicken colonization by Campylobacter resulting from vaccination was rather moderate, the experiments showed that LAB strains can be considered as an alternative vector to

  14. Estudo da transmigração bacteriana na apendicite experimental em coelhos

    Directory of Open Access Journals (Sweden)

    RAMOS ECF

    2000-01-01

    Full Text Available Introdução: A queda extrínseca dos mecanismos de defesa de um hospedeiro é vista classicamente como uma infecção, que resulta na aquisição de um tecido normal, de um novo microorganismo, cuja virulência é fundamental para a sua patogenia.Na década de 50, estudos comprovaram que bactérias e endotoxinas podem ultrapassar a barreira intestinal e atingir a circulação, demonstrando ainda, que a flora intestinal representa uma importante fonte para o desenvolvimento da infecção sistêmica. Objetivo: o presente estudo visa avaliar a ocorrência de transmigração bacteriana em apendicite experimental induzida em coelhos, mediante a oclusão completa do apêndice vermiforme. Métodos: foram utilizados 24 coelhos brancos, da linhagem Nova Zelândia, com peso corporal variando de 2500 a 3000 gramas, sendo então divididos em 2 grupos. Os animais do Grupo I foram submetidos a laparotomia mediana e retirada de fragmentos do baço, rim esquerdo, pulmão direito, linfonodo abdominal, conteúdo da luz do apêndice vermiforme e sangue da veia porta para cultura. Os animais do Grupo II foram submetidos a laparotomia mediana e oclusão completa do apêndice vermiforme para a indução de apendicite e, após 24 horas, realizada a coleta do material nos moldes do Grupo I. Resultados: No Grupo controle (Grupo I, não se observou o crescimento de bactérias nas amostras analisadas. No Grupo experimento (Grupo II, observou-se o crescimento de Escherichia coli em todos os animais. Conclusão: Ocorreu transmigração bacteriana após 24 horas de observação em coelhos com apendicite experimental.

  15. Novel Food-Grade Plasmid Vector Based on Melibiose Fermentation for the Genetic Engineering of Lactococcus lactis

    OpenAIRE

    Boucher, Isabelle; Parrot, Marc; Gaudreau, Hélène; Champagne, Claude P.; Vadeboncoeur, Christian; Moineau, Sylvain

    2002-01-01

    The α-galactosidase gene (aga) and a gene coding for a putative transcriptional regulator from the LacI/GalR family (galR) of Lactococcus raffinolactis ATCC 43920 were cloned and sequenced. When transferred into Lactococcus lactis and Pediococcus acidilactici strains, aga modified the sugar fermentation profile of the strains from melibiose negative (Mel−) to melibiose positive (Mel+). Analysis of galA mutants of L. lactis subsp. cremoris MG1363 indicated that the putative galactose permease ...

  16. Relación placa bacteriana y caries en un grupo de niños escolarizados entre 5 y 14 años de la población de la Boquilla - Cartagena en el año 20041

    Directory of Open Access Journals (Sweden)

    Lizelia Alfaro Zolá

    2013-10-01

    Full Text Available Objetivo: Describir la ocurrencia de placa bacteriana en las superficies dentales y relacionarla con el tipo de lesión de caries que se presenta en niños escolares de 5 a 14 años de la Boquilla - Cartagena. Materiales y Métodos: Se realizó un estudio de corte transversal, en el cual se seleccionó una muestra de 89 niños escolares de la población de la Boquilla a través de un muestreo aleatorio sistemático, a quienes se les evaluó el estado de higiene oral mediante el índice de placa comunitario de Corchuelo modificado y el índice de caries superficial con criterios de diagnóstico Ekstrand. Resultados: La superficie con lesiones de caries de mayor frecuencia fue la oclusal, presentándose un mayor numero de lesiones detenidas, seguido en palatino por las lesiones precavitacionales, a diferencia de las superficies vestibular y lingual donde se encontraron un mayor número de superficies sanas. En todas las superficies evaluadas predominó la presencia de placa bacteriana independientemente de la presencia de caries y no se encontró una relación estadísticamente significativa entre estas dos variables. Conclusiones: La alta frecuencia de placa bacteriana en las superficies dentarias nos mostró el deficiente estado de higiene oral de la población, sin embargo, sigue en duda la pertinencia de los índices de placa para la predicción de la caries debido a que no se encontró relación estadística. (Duazary 2007; 2: 119 - 126

  17. Etiologia e evolução das meningites bacterianas em centro de pediatria Etiology and evolution of bacterial meningitis in a pediatric center

    Directory of Open Access Journals (Sweden)

    Roberta M.C. Romanelli

    2002-02-01

    Full Text Available Objetivo: determinar a prevalência dos agentes etiológicos das meningites bacterianas em serviço de referência, no atendimento de doenças infecciosas para o estado de Minas Gerais, e verificar a resposta ao tratamento utilizado.Métodos: estudo descritivo em que foram incluídas todas as crianças com diagnóstico provável de meningite, admitidas na instituição no período de junho a novembro/99.Resultados: obteve-se 210 casos de meningite, sendo 111 casos de etiologia bacteriana (52,9%. Destes, 52 casos foram diagnósticos prováveis (por alteração do liquor rotina e 59 com diagnósticos de certeza (por cultura e/ou isolamento de antígeno. Os principais agentes isolados foram, em ordem decrescente, H. influenzae, N. meningitidis e S. pneumoniae. O tratamento inicial para a faixa etária de três meses a cinco anos foi ampicilina e cloranfenicol, sendo posteriormente restrito para penicilina em casos de meningococo e pneumococo, e para cloranfenicol nos casos de H. influenzae. A mudança para antimicrobiano de maior espectro foi realizada com base em dados clínicos ou laboratoriais, não havendo isolamento de microorganismo resistente.Conclusões: o acompanhamento do perfil epidemiológico das meningites deve ser contínuo, e cada serviço deve se basear em dados locais para direcionar a terapia antimicrobiana. A monitorização contínua dos agentes prevalentes em cada instituição e de sua resistência é fundamental para a escolha antimicrobiana, atuando com menor interferência na colonização individual, sem contribuir para a crescente resistência dos agentes responsáveis pelas infecções meníngeas.Objective: to establish the prevalence of the etiological agents of bacterial meningitis in a reference center for the treatment of infectious diseases in the state of Minas Gerais. Methods: descriptive study including all children with probable diagnosis of meningitis between June/1999 and November/1999.Results: there were 210

  18. Medición de cambios cuantitativos de la microbiota subgingival posterior a la remoción de placa bacteriana supragingival Measurement of quantitative changes of the microbiota subgingival after to removal of bacterial plaque supragingival

    Directory of Open Access Journals (Sweden)

    C Godoy

    2010-04-01

    Full Text Available Uno de los campos de interés en el estudio de la microbiología periodontal para muchos investigadores ha sido identificar si es posible que los microorganismos responsables del origen y progresión de la enfermedad periodontal que habitan sobre el margen gingival (supragingival y bajo este (subgingival tengan una relación directa que permita mantener interacciones influyentes en el crecimiento y desarrollo de las diferentes especies bacterianas que habitan en los tejidos periodontales. Por lo tanto, al remover los microorganismos que se ubican supragingivalmente sería posible encontrar cambios en el medio subgingival al no existir un intercambio entre los ambientes aerobios (supragingival y anaerobios (subgingival una vez desorganizada la placa bacteriana supragingival. Para demostrar esta relación se seleccionaron 7 individuos con diagnóstico de periodontitis crónica moderada y severa a los cuales se les realizó un destartraje supragingival de boca completa para lograr desorganizar la placa bacteriana supragingival. A su vez se tomaron muestras microbiológicas de los sacos periodontales más profundos de cada cuadrante de estos individuos, siendo la primera muestra tomada previo al destartraje supragingival considerada como muestra basal (día 0, luego se tomaron a las 24 horas, a los 7 y 21 días de removida la placa bacteriana supragingival. De los resultados del presente estudio pudimos concluir que al desorganizar el biofilm supragingival se observa una disminución en la cantidad total de microorganismos subgingivales, así como también disminuye de manera considerable la proporción de Porphyoromona gingivalis presente en el medio subgingival. Lo cual permitiría establecer la existencia de una relación directa y dependiente entre los microorganismos que habitan el medio supragingival y subgingival.One of the fields of interest in the study of the microbiology periodontal for many investigators has been to identify if it is

  19. Infecciones en pacientes hospitalizados por cirrosis Infections in hospitalized patients with cirrhosis

    Directory of Open Access Journals (Sweden)

    Sebastián Mathurin

    2009-04-01

    Full Text Available Evaluamos la prevalecencia y relevancia clínica de las infecciones bacterianas y no bacterianas en pacientes cirróticos predominantemente alcohólicos internados en un hospital de mediana complejidad, y comparamos las características clínicas, de laboratorio y la evolución de pacientes con y sin infección bacteriana en un estudio prospectivo de cohorte. Se incluyeron 211 internaciones consecutivas de 132 pacientes con diagnóstico de cirrosis, de abril 2004 a julio 2007. El promedio de edad (±DS fue 51.8 (±8 años, 112 fueron hombres (84.8%; etiología alcohólica 95.4%. Se diagnosticaron 129 episodios de infecciones bacterianas en 99/211 (46.9% internaciones, adquiridos en la comunidad 79 (61.2% y 50 (38.8% intrahospitalarios: peritonitis bacteriana espontánea (23.3%; infección urinaria (21.7%; neumonías (17.8%; infecciones de piel y partes blandas (17.1%; sepsis por bacteriemia espontánea (7.7%; otras infecciones bacterianas (12.4%. El 52.2% fueron por gérmenes gram-positivos. Hubo ocho casos de tuberculosis e infecciones graves por hongos y parásitos. La prevalecencia de tuberculosis fue del 6% con una mortalidad anual de 62.5%. El 28.1% (9/32 de los exámenes coproparasitológicos tuvieron Strongyloides stercolaris. La mortalidad hospitalaria fue mayor en los pacientes con infección bacteriana (32.4% vs. 13.2%; p=0.02. Fueron identificados como predictores independientes de mortalidad: las infecciones bacterianas, el score de Child-Pügh y creatininemia > 1.5 mg/dl. En el análisis multivariado fueron factores independientes asociados a infección bacteriana la leucocitosis y la encefalopatía hepática grado III/IV. Este estudio confirma que las infecciones bacterianas y no bacterianas son una complicación frecuente y grave en pacientes cirróticos internados, con un aumento de la mortalidad hospitalaria.We evaluated the prevalence and the clinical relevance of bacterial and nonbacterial infections in predominantly alcoholic

  20. Gene-cassette for adaptation of Lactococcus lactis to a plant environment

    OpenAIRE

    Doman-Pytka, Monika; Renault, Pierre; Bardowski, Jacek

    2004-01-01

    International audience The generally accepted opinion is that the natural niche for lactococci are plants. Several genes reminiscent of the environmental adaptation of these bacteria to the plant habitat were found as a result of our work on the pullulanase coding region in the Lactococcus lactis IBB500 strain. All genes were located within an 11-kb DNA fragment of a 35-kb plasmid. Analysis of the nucleotide sequence of the 11-kb DNA fragment showed three regions: (i) a middle region - enc...

  1. High efficiency electrotransformation of Lactococcus lactis spp. lactis cells pretreated with lithium acetate and dithiothreitol

    OpenAIRE

    Filioussis George; Avramidis Nicholaos; Papagianni Maria

    2007-01-01

    Abstract Background A goal for the food industry has always been to improve strains of Lactococcus lactis and stabilize beneficial traits. Genetic engineering is used extensively for manipulating this lactic acid bacterium, while electropolation is the most widely used technique for introducing foreign DNA into cells. The efficiency of electrotransformation depends on the level of electropermealization and pretreatment with chemicals which alter cell wall permeability, resulting in improved t...

  2. Physiological Adaptation of the Bacterium Lactococcus lactis in Response to the Production of Human CFTR*

    OpenAIRE

    A. Steen; Wiederhold, E.; T Gandhi; Breitling, R.; D. J. Slotboom

    2010-01-01

    Biochemical and biophysical characterization of CFTR (the cystic fibrosis transmembrane conductance regulator) is thwarted by difficulties to obtain sufficient quantities of correctly folded and functional protein. Here we have produced human CFTR in the prokaryotic expression host Lactococcus lactis. The full-length protein was detected in the membrane of the bacterium, but the yields were too low (< 0.1% of membrane proteins) for in vitro functional and structural characterization, and indu...

  3. Physiological function of the maltose operon regulator, MalR, in Lactococcus lactis

    OpenAIRE

    Rådström Peter; Andersson Ulrika

    2002-01-01

    Abstract Background Maltose metabolism is initiated by an ATP-dependent permease system in Lactococcus lactis. The subsequent degradation of intracellular maltose is performed by the concerted action of Pi-dependent maltose phosphorylase and β-phosphoglucomutase. In some Gram-positive bacteria, maltose metabolism is regulated by a maltose operon regulator (MalR), belonging to the LacI-GalR family of transcriptional regulators. A gene presumed to encode MalR has been found directly downstream ...

  4. Bacteriocinogenic Lactococcus lactis subsp. lactis DF04Mi isolated from goat milk: Characterization of the bacteriocin

    OpenAIRE

    Furtado, Danielle N.; Todorov, Svetoslav D.; Mariza Landgraf; Maria T. Destro; Franco, Bernadette D.G.M.

    2015-01-01

    Lactic acid bacteria capable of producing bacteriocins and presenting probiotic potential open innovative technological applications in the dairy industry. In this study, a bacteriocinogenic strain (Lactococcus lactis subsp. lactis DF4Mi) was isolated from goat milk, and studied for its antimicrobial activity. The bacteriocin presented a broad spectrum of activity, was sensitive to proteolytic enzymes, resistant to heat and pH extremes, and not affected by the presence of SDS, Tween 20, Tween...

  5. Development, molecular characterization and exploitation of the nisin controlled expression system in Lactococcus lactis.

    OpenAIRE

    Ruyter, de, D.J.

    1998-01-01

    Lactic acid bacteria are gram-positive bacteria that are widely used in a variety of dairy fermentation processes. Notably, strains of the lactic acid starter bacterium Lactococcus lactis are of great economic importance because of their world-wide use in cheese making. The characteristic aroma, flavor and texture of cheese develops during ripening of the cheese curd through the action of numerous enzymes derived from the cheese milk, the coagulant, and the starter and non-starter bacteria. R...

  6. Plasmid biology of natural Lactococcus lactis strains and molecular mechanisms of bacteriophage-host interaction

    OpenAIRE

    Fallico, Vincenzo

    2011-01-01

    Lacticin 3147, enterocin AS-48, lacticin 481, variacin, and sakacin P are bacteriocins offering promising perspectives in terms of preservation and shelf-life extension of food products and should find commercial application in the near future. The studies detailing their characterization and bio-preservative applications are reviewed. Transcriptomic analyses showed a cell wall-targeted response of Lactococcus lactis IL1403 during the early stages of infection with the lytic bacteriophage c2,...

  7. Some chemical and physical properties of nisin, a small-protein antibiotic produced by Lactococcus lactis.

    OpenAIRE

    Liu, W.; Hansen, J N

    1990-01-01

    Nisin is a small gene-encoded antimicrobial protein produced by Lactococcus lactis that contains unusual dehydroalanine and dehydrobutyrine residues. The reactivity of these residues toward nucleophiles was explored by reacting nisin with a variety of mercaptans. The kinetics of reaction with 2-mercaptoethane-sulfonate and thioglycolate indicated that the reaction pathway includes a binding step. Reaction of nisin at high pH resulted in the formation of multimeric products, apparently as a re...

  8. Lactococcus lactis as an adjuvant and delivery vehicle of antigens against pneumococcal respiratory infections

    OpenAIRE

    Medina, Marcela; Vintiñi, Elisa; Villena, Julio; Raya, Raul; Alvarez, Susana

    2010-01-01

    Most studies of Lactococcus lactis as delivery vehicles of pneumococcal antigens are focused on the effectiveness of mucosal recombinant vaccines against Streptococcus pneumoniae in animal models. At present, there are three types of pneumococcal vaccines: capsular polysaccharide pneumococcal vaccines (PPV), protein-polysaccharide conjugate pneumococcal vaccines (PCV) and protein-based pneumococcal vaccines (PBPV). Only PPV and PCV have been licensed. These vaccines, however, do not represent...

  9. Characterization of the Lactococcus lactis lactose genes and regulation of their expression.

    OpenAIRE

    Rooijen, van, J.

    1993-01-01

    An important trait of the lactic acid bacterium Lactococcus lactis , that is used in industrial dairy fermentations, is the conversion of lactose into lactic acid. The enzymatic steps involved in the breakdown of lactose, that is transported into the cell via a phosphoenolpyruvate-dependent lactose phosphotransferase system (PEP-PTS lac), have been well established (Fig. 1). However, except for the molecular cloning and characterization of the plasmid-located phospho-B-galactosidase gene (Boi...

  10. Engineering Trehalose Synthesis in Lactococcus lactis for Improved Stress Tolerance ▿ †

    OpenAIRE

    Carvalho, Ana Lúcia; Cardoso, Filipa S.; Bohn, Andreas; Neves, Ana Rute; Santos, Helena

    2011-01-01

    Trehalose accumulation is a common cell defense strategy against a variety of stressful conditions. In particular, our team detected high levels of trehalose in Propionibacterium freudenreichii in response to acid stress, a result that led to the idea that endowing Lactococcus lactis with the capacity to synthesize trehalose could improve the acid tolerance of this organism. To this end, we took advantage of the endogenous genes involved in the trehalose catabolic pathway of L. lactis, i.e., ...

  11. Engineering of Carbon Distribution between Glycolysis and Sugar Nucleotide Biosynthesis in Lactococcus lactis

    OpenAIRE

    Boels, Ingeborg C.; Kleerebezem, Michiel; de Vos, Willem M.

    2003-01-01

    We describe the effects of modulating the activities of glucokinase, phosphofructokinase, and phosphoglucomutase on the branching point between sugar degradation and the biosynthesis of sugar nucleotides involved in the production of exopolysaccharide biosynthesis by Lactococcus lactis. This was realized by using a described isogenic L. lactis mutant with reduced enzyme activities or by controlled expression of the well-characterized genes for phosphoglucomutase or glucokinase from Escherichi...

  12. Novel Antibacterial Activity of Lactococcus Lactis Subspecies Lactis Z11 Isolated from Zabady

    OpenAIRE

    Enan, Gamal; Abdel-Shafi, Seham; Ouda, Sahar; Negm, Sally

    2013-01-01

    The purpose of this study was to select and characterize a probiotic bacterium with distinctive antimicrobial activities. In this respect, Lactococcus lactis subspecies lactis Z11 (L. lactis Z11) isolated from Zabady (Arabian yoghurt) inhibited other strains of lactic acid bacteria and some food-born pathogens including Listeria monocytogenes, Bacillus cereus and staphylococcus aureus. The inhibitory activity of cell free supernatant (CFS) of L. lactis Z11 isolated from zabady was lost by pro...

  13. Cloning and Characterization of upp, a Gene Encoding Uracil Phosphoribosyltransferase from Lactococcus lactis

    DEFF Research Database (Denmark)

    Martinussen, Jan; Hammer, Karin

    1994-01-01

    Uracil phosphoribosyltransferase catalyzes the key reaction in the salvage of uracil in many microorganisms. The gene encoding uracil phosphoribosyltransferase (upp) was cloned from Lactococcus lactis subsp. cremoris MG1363 by complementation of an Escherichia coli mutant. The gene was sequenced......-negative bacterial strains. The phenotype of the uracil phosphoribosyltransferase-deficient strain was established. Surprisingly, the upp strain is resistant only to very low concentrations of 5-fluorouracil. Secondary mutants in thymidine phosphorylase and thymidine kinase were isolated by selection for resistance...

  14. Purification and Characterization of an Aminopeptidase from Lactococcus lactis subsp. cremoris Wg2

    OpenAIRE

    Tan, P. S. T.; Konings, W. N.

    1990-01-01

    An aminopeptidase was purified to homogeneity from a crude cell extract of Lactococcus lactis subsp. cremoris Wg2 by a procedure that included diethyl-aminoethane-Sephacel chromatography, phenyl-Sepharose chromatography, gel filtration, and high-performance liquid chromatography over an anion-exchange column. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified enzyme showed a single protein band with a molecular weight of 95,000. The aminopeptidase was capable of degradi...

  15. Functional and Morphological Adaptation to Peptidoglycan Precursor Alteration in Lactococcus lactis*

    OpenAIRE

    Deghorain, Marie; Fontaine, Laetitia; David, Blandine; Mainardi, Jean-Luc; Courtin, Pascal; Daniel, Richard; Errington, Jeff; Sorokin, Alexei; Bolotin, Alexander; Chapot-Chartier, Marie-Pierre; Hallet, Bernard; Hols, Pascal

    2010-01-01

    Cell wall peptidoglycan assembly is a tightly regulated process requiring the combined action of multienzyme complexes. In this study we provide direct evidence showing that substrate transformations occurring at the different stages of this process play a crucial role in the spatial and temporal coordination of the cell wall synthesis machinery. Peptidoglycan substrate alteration was investigated in the Gram-positive bacterium Lactococcus lactis by substituting the peptidoglycan precursor bi...

  16. Generation of Dipeptidyl Peptidase-IV-Inhibiting Peptides from β-Lactoglobulin Secreted by Lactococcus lactis

    Directory of Open Access Journals (Sweden)

    Suguru Shigemori

    2014-01-01

    Full Text Available Previous studies showed that hydrolysates of β-lactoglobulin (BLG prepared using gastrointestinal proteases strongly inhibit dipeptidyl peptidase-IV (DPP-IV activity in vitro. In this study, we developed a BLG-secreting Lactococcus lactis strain as a delivery vehicle and in situ expression system. Interestingly, trypsin-digested recombinant BLG from L. lactis inhibited DPP-IV activity, suggesting that BLG-secreting L. lactis may be useful in the treatment of type 2 diabetes mellitus.

  17. The Carbohydrate Metabolism Signature of Lactococcus lactis Strain A12 Reveals Its Sourdough Ecosystem Origin

    OpenAIRE

    Passerini, Delphine; Coddeville, Michèle; Le Bourgeois, Pascal; Loubière, Pascal; Ritzenthaler, Paul; Fontagné-Faucher, Catherine; Daveran-Mingot, Marie-Line; Cocaign-Bousquet, Muriel

    2013-01-01

    Lactococcus lactis subsp. lactis strain A12 was isolated from sourdough. Combined genomic, transcriptomic, and phenotypic analyses were performed to understand its survival capacity in the complex sourdough ecosystem and its role in the microbial community. The genome sequence comparison of strain A12 with strain IL1403 (a derivative of an industrial dairy strain) revealed 78 strain-specific regions representing 23% of the total genome size. Most of the strain-specific genes were involved in ...

  18. Peptide uptake is essential for growth of Lactococcus lactis on the milk protein casein.

    OpenAIRE

    Smid, E. J.; Plapp, R; Konings, W. N.

    1989-01-01

    The chlorated dipeptide L-alanyl-beta-chloro-L-alanine (diACA) is very toxic for Lactococcus lactis. Spontaneous mutants resistant to the dipeptide were isolated from plates. The presence and activities of cell wall-associated proteinase, different peptidases in cell extracts, amino acid transport systems, and di- and oligopeptide transport systems were examined and compared in a diACA-resistant mutant and the wild type. Only the rates of di- and tripeptide transport were found to be signific...

  19. Regulation of Exopolysaccharide Production by Lactococcus lactis subsp. cremoris by the Sugar Source

    OpenAIRE

    Looijesteijn, Petronella J.; Boels, Ingeborg C.; Kleerebezem, Michiel; Hugenholtz, Jeroen

    1999-01-01

    Lactococcus lactis produced more exopolysaccharide (EPS) on glucose than on fructose as the sugar substrate, although the transcription level of the eps gene cluster was independent of the sugar source. A major difference between cells grown on the two substrates was the capacity to produce sugar nucleotides, the EPS precursors. However, the activities of the enzymes required for the synthesis of nucleotide sugars were not changed upon growth on different sugars. The activity of fructosebisph...

  20. Prevention of gastrointestinal lead poisoning using recombinant Lactococcus lactis expressing human metallothionein-I fusion protein

    OpenAIRE

    Xue Xiao; Changbin Zhang; Dajun Liu; Weibin Bai; Qihao Zhang; Qi Xiang; Yadong Huang; Zhijian Su

    2016-01-01

    Low-level lead poisoning is an insidious disease that affects millions of children worldwide, leading to biochemical and neurological dysfunctions. Blocking lead uptake via the gastrointestinal tract is an important prevention strategy. With this in mind, we constructed the recombinant Lactococcus lactis strain pGSMT/MG1363, which constitutively expressed the fusion protein glutathione S-transferase (GST)–small molecule ubiquitin-like modifier protein (SUMO)–metallothionein-I (GST-SUMO-MT). T...

  1. Síntesis intratecal de C3c e inmunoglobulinas en niños con meningoencefalitis bacteriana

    OpenAIRE

    Bárbara Padilla-Docal; Alberto Juan Dorta-Contreras; Raisa Bu Coifiu-Fanego; Elena Noris-García; Hermes Fundora-Hernández; Jesus Callol-Barroso; Marlen González-Hernández

    2008-01-01

    Las meningoencefalitis bacterianas constituyen una fuente importante de morbilidad, mortalidad y discapacidad en diferentes regiones del mundo. El objetivo del presente trabajo es conocer si el sistema de complemento puede estar involucrado en la lisis de las bacterias productoras de meningoencefalitis a través de la liberación de C3c al líquido cefalorraquídeo. Se estudiaron siete pacientes con edad promedio de 3 años, que ingresaron en el Hospital Pediátrico de San Miguel del Padrón, a los ...

  2. Efecto de cisapride sobre el sobrecrecimiento bacteriano intestinal y la translocación bacteriana en la cirrosis

    OpenAIRE

    Pardo Balteiro, Alberto

    2003-01-01

    La alteración de la motilidad intestinal que se detecta con frecuencia en la cirrosis puede facilitar la presencia de sobrecrecimiento bacteriano intestinal (SBI). Este, a su vez, puede conducir a la aparición de translocación bacteriana (TB). El objetivo principal del estudio fue evaluar el efecto de la administración de cisapride sobre el SBI y la TB en la cirrosis. Se utilizó el modelo de cirrosis experimental en ratas mediante la administración de tetracloruro de carbono. Dos grupos de r...

  3. Etiologia e evolução das meningites bacterianas em centro de pediatria Etiology and evolution of bacterial meningitis in a pediatric center

    OpenAIRE

    Roberta M. C. Romanelli; Claudete A. Araújo; Fernando Boucinhas; Inácio R. Carvalho; Nelson R.L. Martins; Heliane B. M. Freire

    2002-01-01

    Objetivo: determinar a prevalência dos agentes etiológicos das meningites bacterianas em serviço de referência, no atendimento de doenças infecciosas para o estado de Minas Gerais, e verificar a resposta ao tratamento utilizado.Métodos: estudo descritivo em que foram incluídas todas as crianças com diagnóstico provável de meningite, admitidas na instituição no período de junho a novembro/99.Resultados: obteve-se 210 casos de meningite, sendo 111 casos de etiologia bacteriana (52,9%). Destes, ...

  4. Estomatitis ulcerativa infecciosa como consecuencia de un traumatismo en una Boa constrictor con desarrollo de neumonía bacteriana y septicemia.

    OpenAIRE

    Zulma Esperanza Rojas-Sereno; Ximena Gómez-Acosta; Claudia Brieva-Rico

    2015-01-01

    Las neumonías bacterianas se asocian con estomatitis en serpientes, debido al descenso de los exudados de la cavidad hacia el pulmón. El objetivo de este reporte es describir los hallazgos clínicos, y el manejo diagnóstico y terapéutico de una Boa constrictor con estomatitis ulcerativa infecciosa (EUI) postraumática que se complicó con neumonía bacteriana (NB) y septicemia (SE). Un adulto de B. constrictor, ingresó con mucosas congestionadas, presencia de tierra en la cavidad oral, ulceracion...

  5. Aglutinación de partículas de látex vs. contrainmunoelectroforesis en meningitis bacteriana aguda Latex agglutination vs. counterimmunoelectrophoresis in the diagnosis of acute bacterial meningitis

    Directory of Open Access Journals (Sweden)

    Carmen Tulia Zapata Muñoz

    1991-01-01

    Full Text Available Se estudiaron 57 pacientes con meningitis aguda, de etiología bacteriana comprobada; 47.4% (27 casos fueron causados por Haemophilus influenzae tipo b; 21.0% (12 casos por Streptococcus pneumoniae; 17.5% (10 casos por Neisseria meningitidis; 5.3% (3 casos por Staphylococcus aureus,. 5.3% (3 casos por enterobacterias y 3.5% (2 casos por gérmenes no Identificados por cultivos. Se comparó la aglutinación de partículas de látex (APL con la contralnmunoelectroforesis (CIE en los pacientes con cultivo positivo. La exactitud de ambas fue similar para el H. influenzae tipo b y el S. pneumoniae. Tres de los 10 casos con cultivo positivo para N. meningítidis fueron positivos en la APL pero ninguno lo fue en la CIE. Se presentó un falso positivo para H. ínfluenzae con la APL que correspondió a meningitis por Salmonella typhí, Las pruebas inmunológicas estuvieron plenamente justificadas en 12 de los 57 pacientes (21.0%, previamente tratados, en quienes la bacteriología tradicional fue negativa o se quería identificar el germen porque lo único positivo era el gram y se justificaba utilizar el antibiótico más especifico. Se sugiere el uso de la APL en el Hospital Infantil de Medellín, por ser una prueba confiable y más simple y rápida que la CIE.

    A comparison was made between latex particles agglutination (LPA and counterimmunoelectrophoresis (CIE in the diagnosis of 57 children with acute bacterial meningitis; reagents were utllized to detect infection by Haemophilus influenzae, Streptococcus pneumoniae and Neísseria meningitídís. Results of both tests were similar for diagnosis of H. ínfluenzae and S. pneumoniae; in contrast only 30.0% of cases due to N. meningitidis gave a positive result with LP A and none was detected with CIE.in 12 patients (21.0% LPA and CIE were the only tests that allowed a precise determination ot the etiology of the disease. The authors

  6. Perfil clínico e microbiológico de mulheres com vaginose bacteriana Clinical and microbiological profile of women with bacterial vaginosis

    Directory of Open Access Journals (Sweden)

    Sonia Regina Ribeiro de Figueiredo Leite

    2010-02-01

    Full Text Available OBJETIVO: estudar o perfil clínico e microbiológico de mulheres portadoras de vaginose bacteriana participantes de um ensaio clínico randomizado, duplamente mascarado, que comparou aroeira e metronidazol, em uso vaginal, para tratamento do corrimento genital. MÉTODOS: o estudo constitui-se em uma série de casos de 277 mulheres portadoras de vaginose bacteriana diagnosticada, concomitantemente, pelos critérios de Amsel e Nugent, selecionadas a partir de um total de 462 recrutadas, utilizando as informações colhidas antes da intervenção. A análise dos dados foi efetuada utilizando-se o programa Epi-Info 3.32. Para comparar as frequências dos desfechos entre os grupos de intervenção, foi utilizado o teste do χ2 e foi calculada a razão de risco e o intervalo de confiança a 95%. Foi feita análise por intenção de tratar. Além dos parâmetros de diagnósticos, foram também colhidas cultura do conteúdo vaginal e uma citologia de Papanicolaou. RESULTADOS: entre as queixas clínicas, as mais frequentes foram o corrimento genital, observado em 206 participantes (74,4% e o odor de peixe da secreção vaginal, que ocorreu em 68,6% dos casos (190 pacientes. Dentre os critérios clínicos de diagnósticos, a presença de clue-cells foi positiva em 275 mulheres (99,3%, o teste de Whiff positivo apareceu em 266 participantes (96,0%, seguido do pH >4,5, que ocorreu em 92,8% dos casos e da presença de corrimento fluido e acinzentado, citado por 206 participantes (74,4%. Com relação ao critério de Nugent, a mediana dos escores foi o valor 8,0. As culturas de conteúdo vaginal permitiram a identificação de Gardnerella vaginalis em 96,8% e de Mobiluncus, em 53,1% dos casos. Apenas uma terça parte dos exames mostrou a presença de Lactobacillus (89 mulheres - 32,1%. Houve crescimento de fungos em culturas de 14 participantes (5,1%. Na maior parte dos casos, os resultados das culturas demonstraram a presença de Corynebacterium (94

  7. Inoculação de suspensão bacteriana de Plesiomonas shigelloides em Jundiá, Rhamdia quelen (Teleostei: Pimelodidae Inoculation of bacterial suspension of Plesiomonas shigelloides in jundiá, Rhamdia quelen (Teleostei: Pimelodidae

    Directory of Open Access Journals (Sweden)

    Cheila de Lima Boijink

    2001-06-01

    Full Text Available Com o crescimento da aqüicultura mundial e intensificação da criação de peixes, os animais ficam sujeitos às enfermidades bacterianas e outras. Com o objetivo de avaliar a patogenicidade da Plesiomonas shigelloides para jundiás (Rhamdia quelen, diferentes concentrações bacterianas (3 x 10(8 e 9 x 10(8 UFC - Unidade Formadora de Colônia/ml foram inoculadas por via intraperitoneal. Foram utilizados 84 jundiás juvenis com peso e comprimento médios de 24,37 ± 4,28g e 14,42 ± 1,62cm, respectivamente. Os animais inoculados foram mantidos durante 21 dias, em caixas d'agua de amianto, em condições semelhantes de temperatura, pH, alcalinidade e dureza. Os jundiás foram sacrificados a cada dois dias para contagem de UFC/ml de tecido renal. Por observações diárias, constatou-se que a inoculação intraperitoneal de Plesiomonas shigelloides não ocasionou nenhuma alteração nos jundiás, independente da concentração inoculada. As contagens das bactérias nos rins dos jundiás mantiveram-se entre 10(5 e 10(6UFC/ml até o 21º dia, quando o experimento foi finalizado.As worldwide aquaculture has grown, and intensification in fish raising, the animals are subject to bacterial diseases and others. With the aim of evaluating pathogenicity of Plesiomonas shigelloides for "jundiá" (Rhamdia quelen, different bacterial concentrations (3 x 10(8 e 9 x 10(8 CFU - Colony Former Unit/ml were inoculated via peritoneum. Eigthy four juvenile "jundiá" averaging 24.37 ± 4,28g of weight and 14.42 ± 1,62cm of length were utilized. The inoculated animals were maintained for 21 days, in asbestos water tanks, at similar temperature, pH, alkalinity and hardness conditions. The "jundiás" were slaughtered every other day for counting UFC/ml renal tissue. For daily inspections, it was observed that intraperitoneal inoculation of Plesiomonas shigelloides did not cause any change in the catfishes, regardless inoculated concentration. Bacteria counting in

  8. Lactococcus lactis NCC 2287 Alleviates Food Allergic Manifestations in Sensitized Mice by Reducing IL-13 Expression Specifically in the Ileum

    Directory of Open Access Journals (Sweden)

    Adrian W. Zuercher

    2012-01-01

    Full Text Available Objective. Utilizing a food allergy murine model, we have investigated the intrinsic antiallergic potential of the Lactococcus lactis NCC 2287 strain. Methods. BALB/c mice were sensitized at weekly intervals with ovalbumin (OVA plus cholera toxin (CT by the oral route for 7 weeks. In this model, an oral challenge with a high dose of OVA at the end of the sensitization period leads to clinical symptoms. Lactococcus lactis NCC 2287 was given to mice via the drinking water during sensitization (prevention phase or after sensitization (management phase. Results. Lactococcus lactis NCC 2287 administration to sensitized mice strikingly reduced allergic manifestations in the management phase upon challenge, when compared to control mice. No preventive effect was observed with the strain. Lactococcus lactis NCC 2287 significantly decreased relative expression levels of the Th-2 cytokine, IL-13, and associated chemokines CCL11 (eotaxin-1 and CCL17 (TARC in the ileum. No effect was observed in the jejunum. Conclusion/Significance. These results taken together designate Lactococcus lactis NCC 2287 as a candidate probiotic strain appropriate in the management of allergic symptoms.

  9. Factores para el escalado del proceso de producción de celulosa por fermentación estática

    OpenAIRE

    Luis Alfonso Caicedo; Da França, F. P.; Lopez, L.

    2011-01-01

    En la producción de celulosa bacteriana por el método estático, el factor de rendimiento Yp/s es afectado por la concentración de glucosa inicial y el tiempo de fermentación. Bajas concentraciones dan valores altos de Yp/s al inicio de la fermentación, mientras que altas concentraciones requieren tiempos largos para lograr valores comparables. El área superficial aumenta la producción de celulosa, pero existe una relación (área interfacial/volumen de medio) límite a partir de la cual la celul...

  10. por valores

    Directory of Open Access Journals (Sweden)

    Jazmín Díaz-Barrios

    2005-01-01

    Full Text Available La era actual se caracteriza por una búsqueda de la identidad del hombre, donde el pivote del cambio organizacional es el individuo; cambiar en este entorno depende, en gran medida, de los valores que imperen en la cultura de la organización. Este trabajo documental intenta identificar aquellos valores, condición sine-qua-non, para el logro de los objetivos de cambio y analizarlos a fin de determinar sus indicadores, con el objeto de presentar una herramienta a las organizaciones que les facilite adaptarse a los nuevos tiempos. Se encontró que los valores: Delegación, Comunicación, Colaboración, Participación y Aprendizaje, son esenciales en los cambios integrales de esta era. Se determinaron 20 indicadores de su presencia. Se concluye que con esta información cada ente puede elaborar instrumentos que le permitan saber si los valores requeridos están presentes y en caso contrario tomar decisiones que alineen a la organización alrededor de los valores humanistas planteados, incrementando así las posibilidades de éxito en el proceso de cambio y por ende, las posibilidades de supervivencia en esta nueva época.

  11. Control of Brochothrix thermosphacta in pork meat using Lactococcus lactis subsp. lactis I23 isolated from beef

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    Olusegun A Olaoye

    2015-06-01

    Full Text Available This study evaluated the antimicrobial activities of two lactic acid bacteria (LAB Lactococcus lactis subsp. lactis I23 and L. lactis subsp. hordinae E91 against Brochothrix thermosphacta in pork during storage at ambient temperature (30oC over 7 days. Both the LAB strains and spoilage organism were inoculated on fresh pork samples at 1x106cfu/g. About 3 log reduction in the spoilage organism was obtained in LAB treated samples after 48 h of storage. The spoilage organism was confirmed to be sensitive to the bacteriocin nisin produced by Lactococcus lactis subsp. lactis I23. There were reductions in the counts of Salmonella typhimurium, Listeria monocytogenes, Enterobacteriaceae and Staphylococcus in the treated samples. Conclusively, growth of B. thermosphacta could be effectively controlled by nisin producing Lactococcus lactis subsp. lactis I23 in fresh pork during storage, thereby enhancing shelf life of the product.

  12. Activities of amylase, proteinase, and lipase enzymes from Lactococcus chungangensis and its application in dairy products.

    Science.gov (United States)

    Konkit, Maytiya; Kim, Wonyong

    2016-07-01

    Several enzymes are involved in the process of converting milk to lactic acid and coagulated milk to curd and, therefore, are important in dairy fermented products. Amylase, proteinase, and lipase are enzymes that play an important role in degrading milk into monomeric molecules such as oligosaccharides, amino acids, and fatty acids, which are the main molecules responsible for flavors in cheese. In the current study, we determined the amylase, proteinase, and lipase activities of Lactococcus chungangensis CAU 28(T), a bacterial strain of nondairy origin, and compared them with those of the reference strain, Lactococcus lactis ssp. lactis KCTC 3769(T), which is commonly used in the dairy industry. Lactococcus chungangensis CAU 28(T) and L. lactis ssp. lactis KCTC 3769(T) were both found to have amylase, proteinase, and lipase activities in broth culture, cream cheese, and yogurt. Notably, the proteinase and lipase activities of L. chungangensis CAU 28(T) were higher than those of L. lactis ssp. lactis KCTC 3769(T), with proteinase activity of 10.50 U/mL in tryptic soy broth and 8.64 U/mL in cream cheese, and lipase activity of 100 U/mL of tryptic soy broth, and 100 U/mL of cream cheese. In contrast, the amylase activity was low, with 5.28 U/mL in tryptic soy broth and 8.86 U/mL in cream cheese. These enzyme activities in L. chungangensis CAU 28(T) suggest that this strain has potential to be used for manufacturing dairy fermented products, even though the strain is of nondairy origin. PMID:27108177

  13. Exploring optimization parameters to increase ssDNA recombineering in Lactococcus lactis and Lactobacillus reuteri

    OpenAIRE

    van Pijkeren, Jan-Peter; Neoh, Kar Mun; Sirias, Denise; Findley, Anthony S.; Britton, Robert A.

    2012-01-01

    Single-stranded DNA (ssDNA) recombineering is a technology which is used to make subtle changes in the chromosome of several bacterial genera. Cells which express a single-stranded DNA binding protein (RecT or Bet) are transformed with an oligonucleotide which is incorporated via an annealing and replication-dependent mechanism. By in silico analysis we identified ssDNA binding protein homologs in the genus Lactobacillus and Lactococcus lactis. To assess whether we could further improve the r...

  14. Complete genome sequence and description of Lactococcus garvieae M14 isolated from Algerian fermented milk

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    M. Moumene

    2016-03-01

    Full Text Available We describe using a polyphasic approach that combines proteomic by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF analysis, genomic data and phenotypic characterization the features of Lactococcus garvieae strain M14 newly isolated from the fermented milk (known as raib of an Algerian cow. The 2 188 835 bp containing genome sequence displays a metabolic capacity to form acid fermentation that is very useful for industrial applications and encodes for two bacteriocins responsible for its eventual bioprotective properties.

  15. Cloning, nucleotide sequence, and regulatory analysis of the Lactococcus lactis dnaJ gene.

    OpenAIRE

    van Asseldonk, M; Simons, A.; Visser, H.; DE VOS W.M.; Simons, G

    1993-01-01

    The dnaJ gene of Lactococcus lactis was isolated from a genomic library of L. lactis NIZO R5 and cloned into pUC19. Nucleotide sequencing revealed an open reading frame of 1,137 bp in length, encoding a protein of 379 amino acids. The deduced amino acid sequence showed homology to the DnaJ proteins of Escherichia coli, Mycobacterium tuberculosis, Bacillus subtilis, and Clostridium acetobutylicum. The level of the dnaJ monocistronic mRNA increased approximately threefold after heat shock. The ...

  16. Effects of Lactococcus lactis on composition of intestinal microbiota: Role of nisin

    DEFF Research Database (Denmark)

    Bernbom, Nete; Licht, Tine Rask; Brogren, Carl-Henrik;

    2006-01-01

    This study examined the ability of (i) pure nisin, (ii) nisin-producing Lactococcus lactis strain CHCC5826, and (iii) the non-nisin-producing L. lactis strain CHCH2862 to affect the composition of the intestinal microbiota of human flora-associated rats. The presence of both the nisin-producing and...... intestine and 200-fold higher in feces than the corresponding concentrations estimated by a biological assay. This indicates that nisin was degraded or inactivated in the gastrointestinal tract, since fragments of this bacteriocin are detected by ELISA while an intact molecule is needed to retain biological...

  17. Elucidating Flux Regulation of the Fermentation Modes of Lactococcus lactis:A Mutlilevel Study

    OpenAIRE

    Chan, Siu Hung Joshua; Solem, Christian; Jensen, Peter Ruhdal

    2014-01-01

    De mange års anvendelse af mælkesyrebakterien Lactococcus lactis (L. lactis) indenfor mejeriindustrien, har været medvirkende til at L. lactis er blevet en af de mest velkarakteriserede bakterier. Denne Gram positive bakterie, som har et lavt GC indhold, har en relativt simpel metabolisme og er let at modificere genetisk. Dette har gjort den til et attraktivt mål for ”metabolic engineering”, bl.a. med henblik på produktion af non-food relaterede kemikalier. Derudover har den status som den fø...

  18. Secretion of biologically active murine interleukin-2 by Lactococcus lactis subsp. lactis.

    OpenAIRE

    Steidler, L; Wells, J M; Raeymaekers, A; Vandekerckhove, J; Fiers, W; Remaut, E

    1995-01-01

    Secretion of functional recombinant murine interleukin-2 (mIL2) by Lactococcus lactis was achieved by fusion of the sequence encoding mature mIL2 to the secretion signal leader of the lactococcal usp45 gene placed under transcriptional control of the phage T7 promoter-T7 RNA polymerase expression system. The recombinant mature mIL2 was one of only a few proteins which accumulated in the growth medium. Sequence analysis revealed correct processing at the first amino acid of the mature protein....

  19. Oxidative Stress at High Temperatures in Lactococcus lactis Due to an Insufficient Supply of Riboflavin

    OpenAIRE

    Chen, Jun; Shen, Jing; Solem, Christian; Jensen, Peter Ruhdal

    2013-01-01

    Lactococcus lactis MG1363 was found to be unable to grow at temperatures above 37°C in a defined medium without riboflavin, and the cause was identified to be dissolved oxygen introduced during preparation of the medium. At 30°C, growth was unaffected by dissolved oxygen and oxygen was consumed quickly. Raising the temperature to 37°C resulted in severe growth inhibition and only slow removal of dissolved oxygen. Under these conditions, an abnormally low intracellular ratio of [ATP] to [ADP] ...

  20. Synthesis and Posttranslational Regulation of Pyruvate Formate-Lyase in Lactococcus lactis

    OpenAIRE

    Melchiorsen, Claus Rix; Jokumsen, Kirsten Væver; Villadsen, John; Johnsen, Mads G.; Israelsen, Hans; Arnau, José

    2000-01-01

    The enzyme pyruvate formate-lyase (PFL) from Lactococcus lactis was produced in Escherichia coli and purified to obtain anti-PFL antibodies that were shown to be specific for L. lactis PFL. It was demonstrated that activated L. lactis PFL was sensitive to oxygen, as in E. coli, resulting in the cleavage of the PFL polypeptide. The PFL protein level and its in vivo activity and regulation were shown by Western blotting, enzyme-linked immunosorbent assay, and metabolite measurement to be depend...

  1. Genética y genómica enfocadas en el estudio de la resistencia bacteriana Genetics and Genomics for the study of bacterial resistance

    Directory of Open Access Journals (Sweden)

    Ulises Garza-Ramos

    2009-01-01

    Full Text Available La resistencia bacteriana es un problema de salud pública causante de índices elevados de morbi-mortalidad hospitalaria. En la medida en que se usan los diferentes antibióticos se seleccionan bacterias resistentes a múltiples fármacos. El desarrollo de nuevas herramientas moleculares de la genómica y proteómica, como el PCR en tiempo real, pirosecuenciación de ADN, espectrometría de masas, microarreglos de ADN y bioinformática, permite conocer en forma más estrecha la fisiología y estructura de las bacterias y los mecanismos de resistencia a los antibióticos. Estos estudios hacen posible identificar nuevos blancos farmacológicos y diseñar antibióticos específicos para suministrar tratamientos más certeros que combatan las infecciones producidas por bacterias. Con estas técnicas también es posible la identificación rápida de los genes que confieren la resistencia a los antibióticos y el reconocimiento de las estructuras genéticas complejas como los integrones, que intervienen en la diseminación de los genes que producen la multirresistencia.Bacterial resistance is a public health problem causing high rates of morbidity and mortality in hospital settings. To the extent that different antibiotics are used, bacteria resistant to multiple drugs are selected. The development of new molecular genomic and proteomic tools such as real-time PCR, DNA pyrosequencing, mass spectrometry, DNA microarrays, and bioinformatics allow for more in-depth knowledge about the physiology and structure of bacteria and mechanisms involved in antibiotic resistance. These studies identify new targets for drugs and design specific antibiotics to provide more accurate treatments to combat infections caused by bacteria. Using these techniques, it will also be possible to rapidly identify genes that confer resistance to antibiotics, and to identify complex genetic structures, such as integrons that are involved in the spread of genes that confer

  2. Validación de nuevos parámetros predictivos de infecciones bacterianas severas en niños febriles menores de 36 meses de edad

    Directory of Open Access Journals (Sweden)

    Alicia Álvarez Rodríguez

    1999-12-01

    Full Text Available Se realizó una investigación descriptiva-prospectiva con el objetivo de validar nuestros criterios de riesgos en la identificación de la infección bacteriana severa (IBS en niños febriles, para lo cual se establecieron las categorías de riesgos y se aplicaron en 215 niños febriles que habían ingresado por esta causa en un período de 12 meses. Se obtuvo que el 54,9 % de los niños se clasificaron como bajo riesgo y el 71,6 % sin IBS. No obstante, la frecuencia de dicha afección resultó elevada, 28,4 %, con predominio de las infecciones urinarias. Se triplicó el riesgo de padecer IBS en los niños con aspecto tóxico, temperatura mayor o igual a 39°C, test clínico de Bonadio mayor que 8, cituria mayor o igual a 10 000 células/mm³, con diferencias significativas entre las categorías de riesgo y la IBS, y fue mayor la frecuencia en los niños tóxicos y de riesgos, 92,8 y 54,3 %, respectivamente. Uno de cada 4 niños febriles presentó IBS. Se introdujo la categoría "Riesgo" y se recomienda un flujograma de evaluación.A descriptive-prospective research study was carried out to validate our risk criteria in the identification of severe bacterial infection in febrile young children. For this purpose, risk categories were set and applied in 215 febrile children who had been admitted in a period of 12 months. 54.9 % of febrile children were at low risk for serious bacterial infections whereas 71.6 % presented no risk at all. Nevertheless, the frequency of this affection was high, accounting for 28.4 % with predominance of urinary infections. The risk of getting SBI trippled in children who shared toxic look, temperature greater than or equal to 39 °C, Bonadium clinical test higher than 8, cyturia values over or equal to 10 000 cell/mm³, significant differences between risk and SBI categories and the highest frequency of such affection found in toxic children and in children at low risk (92.8 vs 54.3 %. One in every four febrile

  3. Tamanho do tubérculo-semente de batata não interfere na manifestação da murcha bacteriana Potato seed tuber size does not interfere with the incidence of potato bacterial wilt

    Directory of Open Access Journals (Sweden)

    Carlos A Lopes

    2011-06-01

    Full Text Available A batata-semente é comercializada no Brasil em diferentes tamanhos, conforme determinação do MAPA, variando do tipo 0 (zero, com tubérculos acima de 60 mm, até o tipo V, com tubérculos menores que 23 mm. Neste trabalho, foi avaliado o efeito do tamanho do tubérculo da batata-semente na manifestação da murcha bacteriana nas cultivares Agata, BRS Ana, Asterix e Bintje, em campo naturalmente infestado com Ralstonia solanacearum, em Brasília-DF. Foram usados tubérculos pequenos, médios e grandes, com aproximadamente 30, 50 e 90 mm em média, tamanhos correspondentes respectivamente aos tipos IV, II e 0 das normas do MAPA. Diferenças significativas quanto à incidência da doença foram encontradas entre cultivares: BRS Ana e Asterix não diferiram entre si e foram mais resistentes que Agata e Bintje que, por sua vez, tampouco diferiram entre si. As diferenças entre tamanhos de tubérculos e as interações entre cultivar e tamanho de tubérculos não foram significativas. Portanto concluiu-se que o tamanho de tubérculos dentro dos limites avaliados neste trabalho, não interferiu na manifestação da doença em testes de avaliação para resistência à murcha bacteriana.Potato seed tubers in Brazil are commercialized in different sizes as determined by the Ministry of Agriculture, Livestock and Food Supply. The sizes are classified in types, which vary from type 0 (zero, tubers above 60 mm, up to type V, tubers below 23 mm. In this work, we evaluated the influence of seed tuber size on the incidence of bacterial wilt on cultivars Agata, BRS Ana, Asterix, and Bintje, grown in a field naturally infested with Ralstonia solanacearum, in Brasilia, Brazil. We used small, medium and large tubers, measuring in average 30, 50 and 90 mm, which correspond respectively to types IV, II and 0 according to the Brazilian legislation. Significant differences in bacterial wilt incidences were detected among cultivars: BRS Ana and Asterix did not

  4. Bacterial translocation in an experimental intestinal obstruction model: C-reactive protein reliability? Translocação bacteriana no modelo experimental de obstrução intestinal: A proteína C-reativa é confiável?

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    Saleh Ibrahim El-Awady

    2009-04-01

    Full Text Available BACKGROUND: Bacterial translocation occurs in preseptic conditions such as intestinal obstruction through unclear mechanism. The C-reactive protein is an acute phase reactant and a marker of ischemia. METHODS: 45 albino male rats were divided into 3 groups each 15 rats. GI control, GII simple intestinal-obstruction and GIII strangulated obstruction. Outcome measures were: (1 Bacteriologic count and typing for intestinal contents, intestinal wall, liver, mesenteric lymph nodes and blood (cardiac and portal (2 Histopathologic: mucosal injury score, inflammatory cell infiltrate in the wall, MLN, liver, (3 Biochemical: serum CRP, IL-10, mucosal stress pattern (glutathione peroxidase-malonyldialdhyde tissue levels. RESULTS: (1 Intestinal obstruction associates with BT precursors (Bact-overgrowth, mucosal-acidosis, immuno-incomptence, (2 Bacterial translocation (frequency and density was found higher in strangulated I.O, that was mainly enteric (aerobic and anaerobic and mostly E.coli, (3 The pathogen commonality supports the gut origin hypothesis but the systemic inflammatory response goes with the cytokine generating one. (4 The CRP median values for GI, II, III were 0.5, 6.9, 8.5 mg/L, for BT +ve 8 mg/L and 0.75 mg/L for BT -ve rats. CONCLUSION: Bacterial translocation occurs bi-directional (systemic-portal in intestinal obstruction and the resultant inflammatory response pathogenesis is mostly 3 hit model. The CRP is a non selective marker of suspected I.O cases. However, it is a reliable marker of BT, BT density and vascular compromise during I.O.OBJETIVO: Translocação bacteriana ocorre em condições pré-sépticas como na obstrução intestinal por mecanismo não esclarecido. A proteína C-reativa é um marcador de ischemia em fase aguda. A proposição é investigar os possíveis efeitos da obstrução intestinal no equilíbrio ecológico microbiano. MÉTODOS: 45 ratos machos albinos foram distribuídos em três grupos de 15 ratos. GI

  5. Efeito de produtos químicos e biológicos sobre a mancha bacteriana, flora microbiana no filoplano e produtividade de pimentão Effects of chemical and biological products on bacterial spot, microbial flora and yield in bell pepper

    Directory of Open Access Journals (Sweden)

    Débora AG da Silva

    2006-06-01

    Full Text Available Avaliou-se o efeito de pulverizações semanais com sulfato de estreptomicina + oxitetraciclina (0,8 g ia L-1, oxicloreto de cobre (2,4 g ia L-1, biofertilizante Agrobio (5%, e testemunha (água sobre o controle da mancha-bacteriana, sobre a flora microbiana no filoplano e sobre a produtividade de três cultivares de pimentão (Magda, Cascadura Itaipu e Magali R. Os experimentos foram realizados em campo de maio a outubro de 2002. Adotou-se o delineamento de blocos ao acaso, em esquema fatorial (4x3, com quatro repetições. Realizaram-se avaliações semanais, durante três meses para altura de plantas (cm; número de hastes; número total de folhas; número de folhas caídas; incidência de folhas lesionadas; severidade da mancha bacteriana e produtividade (t ha-1. Paralelamente, quantificou-se a população microbiana residente no filoplano e nos frutos. Não houve efeito dos tratamentos sobre a produção de frutos, porém, observou-se efeito sobre o desenvolvimento vegetativo das plantas, sobre a mancha bacteriana e sobre a flora microbiana no filoplano. O oxicloreto de cobre inibiu o desenvolvimento vegetativo, a taxa de abscisão foliar e a flora bacteriana no filoplano. O Agrobio favoreceu o desenvolvimento vegetativo e, assim como o sulfato de estreptomicina + oxitetraciclina, reduziu a população de bactérias, exceto de Bacillus sp., e de fungos, exceto Cladosporium sp., no filoplano. O híbrido Magali R foi significativamente superior às demais cultivares quanto ao vigor das plantas, produtividade e resistência à mancha bacteriana. Estes resultados sugerem atividade bactericida do Agrobio em condições de campo.The effect of weekly sprayings with streptomycin sulfate + oxytetracycline (0.8 g ia L-1, copper oxychloride (2.4 g ia L-1 and Agrobio fertilizer (5% plus a control (water was evaluated in the control of bacterial spot, the microbial flora in the phyloplan, and over yield in three bell pepper cultivars (Magda, Cascadura

  6. Bacterial translocation in rats nonfunctioning diverted distal colon Translocação bacteriana no coto colônico distal desfuncionalizado de ratos

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    Francisco Edilson Leite Pinto Júnior

    2007-06-01

    colite de derivação fecal, seriam capazes de permitir Translocação Bacteriana (TB, ou se a mucosa intestinal atrofiada permitiria a passagem de bactérias para órgãos à distância. MÉTODOS: Foram utilizados 62 ratos Wistar, machos, pesando entre 220 e 320 gramas, divididos em dois grupos: A (Colostomia e B (Controle, contendo cada um 31 animais. No grupo A, os animais foram submetidos à colostomia, terminal boca única, em cólon ascendente. A partir do 70º dia de observação os seguintes procedimentos foram adotados: em cinco ratos foi injetado por via retal no segmento desfuncionalizado - 2ml de uma solução salina 0,9% nos animais (subgrupo A1; em oito inoculou-se, por via retal, uma solução de 2ml contendo Escherichia coli ATCC 25922 (American Type Culture Collection, na concentração de 10(8 Unidades Formadoras de Colônias por mililitros (UFC/ml - Subgrupo A2; em dez animais inoculava-se a mesma solução de E. coli, na concentração de 10(11UFC/ml (Subgrupo A3; e em oito colhia-se o muco do segmento colônico distal desfuncionalizado, para dosagens de açúcares neutros e proteínas totais (subgrupo A4. Os animais do grupo B foram submetidos aos mesmos procedimentos do grupo A, e não foram submetidos à colostomia. Nos animais dos subgrupos A1, A2, A3, B1, B2, e B3, após serem mortos, realizou-se punção cardíaca para coleta de 2ml de sangue e retiraram-se fragmentos de tecidos de linfonodo do mesocólon, fígado, baço, pulmão e rim, para análise microbiológica. Essa análise consistia em evidenciar a presença de UFC de Escherichia coli ATCC 25922. Os testes de Mann-Whitney e Anova foram aplicados como técnicas investigativas para associação das variáveis. RESULTADOS: A ocorrência de TB, só foi evidenciada nos animais em que a concentração inoculada de Escherichia coli ATCC 25922, atingia níveis de 10(11UFC/ml, ou seja, nos Subgrupos A3 e B3, no entanto, sendo significantemente superior (80% nos animais sem colostomia (subgrupo B3

  7. Tulum Peynirlerinden izole Edilen Lactococcus lactis subsp. lactis YBML9 ve

    Directory of Open Access Journals (Sweden)

    Yasin TUNCER

    2009-04-01

    Full Text Available Bu çalısmanın amacı tulum peynirlerinden izole edilen Lactococcus lactis suslarının fenotipik tanısı ve bu suslar tarafından üretilen bakteriyosinlerin kısmi karakterizasyonlarıdır. Bu amaçla Türkiye'nin sekiz farklı ilinden (Ankara, Antalya, Burdur, Denizli, Erzincan, Isparta, İstanbul ve İzmir yöresel pazarlardan toplanan 60 adet tulum peyniri örneginden 40 adet Lactococcus lactis susu (31 adet L. lactis subsp. lactis ve 9 adet L. lactis subsp. cremoris izole edildi. 40 adet L. lactis susu içerisinden, 2 adet L. lactis subsp. lactis (YBML9 ve YBML21 susu bakteriyosin üretme yeteneginde bulundu. L. lactis subsp. lactis YBML9 ve YBML21 susları tarafından üretilen bakteriyosinler, farklı enzim, pH ve sıcaklık uygulamaları sonucu; sırasıyla nisin ve laktisin 481 olarak tanımlandı.

  8. Enhancing bile tolerance improves survival and persistence of Bifidobacterium and Lactococcus in the murine gastrointestinal tract

    Directory of Open Access Journals (Sweden)

    Hill Colin

    2008-10-01

    Full Text Available Abstract Background The majority of commensal gastrointestinal bacteria used as probiotics are highly adapted to the specialised environment of the large bowel. However, unlike pathogenic bacteria; they are often inadequately equipped to endure the physicochemical stresses of gastrointestinal (GI delivery in the host. Herein we outline a patho-biotechnology strategy to improve gastric delivery and host adaptation of a probiotic strain Bifidobacterium breve UCC2003 and the generally regarded as safe (GRAS organism Lactococcus lactis NZ9000. Results In vitro bile tolerance of both strains was significantly enhanced (P Listeria monocytogenes bile resistance mechanism BilE. Strains harbouring bilE were also recovered at significantly higher levels (P n = 5, following oral inoculation. Furthermore, a B. breve strain expressing bilE demonstrated increased efficacy relative to the wild-type strain in reducing oral L. monocytogenes infection in mice. Conclusion Collectively the data indicates that bile tolerance can be enhanced in Bifidobacterium and Lactococcus species through rational genetic manipulation and that this can significantly improve delivery to and colonisation of the GI tract.

  9. Cloning and verification of the Lactococcus lactis pyrG gene and characterization of the gene product, CTP synthase

    DEFF Research Database (Denmark)

    Wadskov-Hansen, Steen Lyders Lerche; Willemoës, M.; Martinussen, Jan;

    2001-01-01

    The pyrG gene of Lactococcus lactis subsp. cremoris, encoding CTP synthase, has been cloned and sequenced. It is flanked upstream by an open reading frame showing homology to several aminotransferases and downstream by an open reading frame of unknown function. L. lactis strains harboring disrupted...

  10. The riboflavin transporter RibU in Lactococcus lactis : Molecular characterization of gene expression and the transport mechanism

    NARCIS (Netherlands)

    Burgess, CM; Slotboom, DJ; Geertsma, ER; Duurkens, Hinderika; Poolman, B; van Sinderen, D

    2006-01-01

    This study describes the characterization of the riboflavin transport protein RibU in the lactic acid bacterium Lactococcus lactis subsp. cremoris NZ9000. RibU is predicted to contain five membrane-spanning segments and is a member of a novel transport protein family, not described in the Transport

  11. BIOLOG MICROLOG® IDENTIFICATION OF Lactococcus garvieae INFECTION IN NILE TILAPIA Oreochromis niloticus AND PINTADO Pseudoplathystoma corruscans FROM BRAZIL

    Science.gov (United States)

    Lactococcus garvieae infection in cultured Nile tilapia, Oreochromis niloticus, and pintado, Pseudoplathystoma corruscans from Brazil is reported. This is the first evidence of the presence of this pathogen from Brazilian fish and the first report of L. garvieae infection in either Nile tilapia or ...

  12. Complete Genome Sequence of Lactococcus lactis subsp. lactis CV56, a Probiotic Strain Isolated from the Vaginas of Healthy Women▿

    OpenAIRE

    Gao, Yong; Lu, Ying; Teng, Kun-Ling; Chen, Mei-Ling; Zheng, Hua-Jun; Zhu, Yong-Qiang; Zhong, Jin

    2011-01-01

    Lactic acid bacteria that exist in the urinogenital system play an important role in maintaining the health of the host. Here, we report the finished and annotated genome of a Lactococcus strain that was isolated from the vaginas of healthy women and shows probiotic properties, including nisin A production and adhesion to vaginal epithelial cells.

  13. Molecular Cloning and Sequence Analysis of the X-Prolyl Dipeptidyl Aminopeptidase Gene From Lactococcus lactis subsp. cremoris

    NARCIS (Netherlands)

    Mayo, Baltasar; Kok, Jan; Venema, Konraad; Bockelmann, Wilhelm; Teuber, Michael; Reinke, Heinz; Venema, Gerhardus

    1991-01-01

    Lactococcus lactis subsp. cremoris P8-2-47 contains an X-prolyl dipeptidyl aminopeptidase (X-PDAP; EC 3.4.14.5). A mixed-oligonucleotide probe prepared on the basis of the N-terminal amino acid sequence of the purified protein was made and used to screen a partial chromosomal DNA bank in Escherichia

  14. Heterologous Gene Expression in Lactococcus lactis subsp. lactis : Synthesis, Secretion, and Processing of the Bacillus subtilis Neutral Protease

    NARCIS (Netherlands)

    Guchte, Maarten van de; Kodde, Jan; Vossen, Jos M.B.M. van der; Kok, Jan; Venema, Gerard

    1990-01-01

    The Bacillus subtilis nprE gene lacking its own promoter sequence was inserted in the lactococcal expression vector pMG36e. Upon introduction of the recombinant plasmid into Lactococcus lactis subsp. lactis strain MG1363, neutral protease activity could be visualized by the appearance of large clear

  15. Complete Genome Sequence of Lactococcus lactis Strain AI06, an Endophyte of the Amazonian Açaí Palm

    OpenAIRE

    McCulloch, John Anthony; de Oliveira, Viviane Matoso; de Almeida Pina, André Vicioli; Pérez-Chaparro, Paula Juliana; Almeida, Lara Mendes; de Vasconcelos, Janaina Mota; de Oliveira, Layanna Freitas; da Silva, Daisy Elaine Andrade; Rogez, Hervé Louis Ghislain; CRETENET, Marina; Mamizuka, Elsa Masae; Nunes, Marcio Roberto Teixeira

    2014-01-01

    We report the genome, in a single chromosome, of Lactococcus lactis strain AI06, isolated from the mesocarp of the açaí fruit (Euterpe oleracea) in eastern Amazonia, Brazil. This strain is an endophyte of the açaí palm and also a component of the microbiota of the edible food product.

  16. The carB gene encoding the large subunit of carbamoylphosphate synthetase from Lactococcus lactis is transcribed monocistronically

    DEFF Research Database (Denmark)

    Martinussen, Jan; Hammer, Karin

    1998-01-01

    The biosynthesis of carbamoylphosphate is catalysed by the heterodimeric enzyme carbamoylphosphate synthetase (CPSase). The genes encoding the two subunits in procaryotes are normally transcribed as an operon, whereas in Lactococcus lactis, the gene encoding the large subunit (carB) is shown to b...

  17. Versatile vector suite for the extracytoplasmic production and purification of heterologous His-tagged proteins in Lactococcus lactis

    NARCIS (Netherlands)

    Neef, Jolanda; Milder, Fin J.; Koedijk, Danny G. A. M.; Klaassens, Marindy; Heezius, Erik C.; van Strijp, Jos A. G.; Otto, Andreas; Becher, Doerte; van Dijl, Jan Maarten; Buist, Girbe

    2015-01-01

    Recent studies have shown that the Gram-positive bacterium Lactococcus lactis can be exploited for the expression of heterologous proteins; however, a versatile set of vectors suitable for inducible extracellular protein production and subsequent purification of the expressed proteins by immobilized

  18. Heterologous Expression and Characterization of an N-Acetyl-beta-D-hexosaminidase from Lactococcus lactis ssp. lactis IL1403

    Czech Academy of Sciences Publication Activity Database

    Nguyen, A. H.; Nguyen, T.-H.; Křen, Vladimír; Eijsink, V. G. H.; Haltrich, D.; Peterbauer, C.

    2012-01-01

    Roč. 60, č. 12 (2012), s. 3275-3281. ISSN 0021-8561 R&D Projects: GA ČR(CZ) GAP207/11/0629 Keywords : N-acetyl-beta-D-hexosaminidase * Lactococcus lactis ssp lactis IL1403 * pNP-GlcNAc Subject RIV: CE - Biochemistry Impact factor: 2.906, year: 2012

  19. Cold shock of Lactococcus lactis MG1363 are involved in cryoprotection and in the production of cold-induced proteins

    NARCIS (Netherlands)

    Wouters, J.A.; Frenkiel, H.; Vos, de W.M.; Kuipers, O.P.; Abee, T.

    2001-01-01

    Members of the group of 7-kDa cold-shock proteins (CSPs) are the proteins with the highest level of induction upon cold shock in the lactic acid bacterium Lactococcus lactis MG1363. By using double-crossover recombination, two L. lactis strains were generated in which genes encoding CSPs are disrupt

  20. Complete Genome Sequence of Lactococcus lactis Strain AI06, an Endophyte of the Amazonian Açaí Palm.

    Science.gov (United States)

    McCulloch, John Anthony; de Oliveira, Viviane Matoso; de Almeida Pina, André Vicioli; Pérez-Chaparro, Paula Juliana; de Almeida, Lara Mendes; de Vasconcelos, Janaina Mota; de Oliveira, Layanna Freitas; da Silva, Daisy Elaine Andrade; Rogez, Hervé Louis Ghislain; Cretenet, Marina; Mamizuka, Elsa Masae; Nunes, Marcio Roberto Teixeira

    2014-01-01

    We report the genome, in a single chromosome, of Lactococcus lactis strain AI06, isolated from the mesocarp of the açaí fruit (Euterpe oleracea) in eastern Amazonia, Brazil. This strain is an endophyte of the açaí palm and also a component of the microbiota of the edible food product. PMID:25414513

  1. Desarrollo de plataformas bacterianas para la biosíntesis de polifenoles

    OpenAIRE

    Gutiérrez del Río Menéndez, Ignacio

    2015-01-01

    Los flavonoides son un grupo mayoritario de polifenoles integrado por 6000 compuestos diferentes ampliamente distribuidos en la dieta debido a su presencia ubicua en plantas. A pesar de que no son nutrientes esenciales para la vida, generan efectos beneficiosos para la salud realizando una importante función en la quimioprevención de enfermedades asociadas con el estrés oxidativo como son el cáncer o las enfermedades cardiovasculares y neurodegenerativas. El creciente interés por estos compue...

  2. Higiene íntima femenina y vaginosis bacteriana: Encuesta Epidemiológica Latinoamericana 2008 Intimate hygiene and bacterial vaginosis: Epidemiological Latinoamerican Survey 2008

    Directory of Open Access Journals (Sweden)

    Aura Cuevas

    2010-09-01

    Full Text Available Objetivos: hacer una aproximación a la prevalencia de vaginosis bacteriana (VB en mujeres en Latinoamérica y explorar la asociación entre el tipo de hábitos higiénicos y prácticas sexuales con la presencia de este diagnóstico. Metodología: estudio de corte transversal en mujeres de 10 países de Latinoamérica entre los 16 y 49 años, vida sexual activa y ciclos menstruales. Se excluyeron las mujeres que presentaban otro tipo de infección vaginal, en gestación o en puerperio, con incapacidad para responder la encuesta o con cambio de pareja sexual en el último trimestre. Igualmente, se realizó muestreo por conveniencia de 1.000 mujeres. Se presenta la prevalencia de punto de VB. Se aplicó una encuesta que contenía información sociodemográfica, información sobre actividad sexual e higiene íntima y se hizo el diagnóstico presuntivo de vaginosis bacteriana basado en criterios clínicos y de laboratorio. Se evalúa la asociación entre VB, los hábitos higiénicos y prácticas sexuales por medio del OR y su intervalos de confianza del 95%. Resultados: la prevalencia de VB fue del 40% en las pacientes encuestadas. Se encontró asociación con el uso de alcohol y tabaco. Como actores protectores se identificaron el lavarse las manos después de orinar o defecar (OR=0,32; IC95%, 0,16-0,63; utilizar una técnica “adecuada” de aseo genital (OR=0,55; IC95%, 0,41-0,74 el uso de jabón en forma líquida (OR=0,62; IC95%, 0,46-0,84 y el uso de un jabón exclusivo para el área genital (OR=0,56; IC95%, 0,42-0,75. Como factores de riesgo se encontraron el uso de tampón (OR=3,00; IC95%,1,88-4,80, tener relaciones sexuales durante el período menstrual (OR=1,46; IC95%, 1,03-2,07 y usar lubricantes durante el coito (OR=1,68; IC 95%, 1,07-2,64. Conclusión: la prevalencia de VB es similar a la previamente informada en Latinoamérica. Su frecuencia esta asociada a los hábitos higiénicos y prácticas sexuales.Objectives: presenting an

  3. Peritonitis bacteriana espontánea: estudio en pacientes cirróticos descompensados con ascitis Spontaneous bacterial peritonitis in decompensated cirrhotic patients with ascites

    Directory of Open Access Journals (Sweden)

    Fernando Montoya Maya

    1995-02-01

    Full Text Available Se realizó un estudio descriptivo prospectivo entre febrero de 1993 y marzo de 1994. Se estudiaron 25 pacientes cirróticos (3 de ellos en dos ocasiones con ascitis clínica o ecográfica que ingresaron al servicio de Medicina Interna del Hospital Universitario San Vicente de Paúl, Medellín, Colombia, por descompensación de su cuadro clínico. A todos se les practicó paracentesis diagnóstica con estudio de citoquímico, citología y cultivo para bacterias aerobias y anaerobias. La edad promedio de los pacientes fue de 43.9 años (13 a 77 afíos, 13 fueron mujeres (52% y 12 hombres (48%. En 11 episodios (39.3% se encontró ascitis infectada, así: ascitis bacteriana 6 casos (54.6% (monomicrobiana en tres y polimicrobiana en otros tres; peritonitis bacteriana espontánea 3 casos (27.2% (polimicrobiana en dos y monomicrobiana en uno y ascitis neutrofílica dos casos (18.2%. El 72.7% de los casos con ascitis infectada tuvieron fiebre contra un 23.5% de aquéllos sin infección (p < 0.014. Asimismo, el dolor abdominal fue un síntoma bastante frecuente (45.5% de infección. La ascitis infectada se presentó únicamente en individuos categoría C de la clasificación de Child-Pugh, con niveles séricos de albúmina, generalmente menores de 2 g/dl (p < 0.01. Los niveles de albúmina en líquido ascítico menores de 1 g/dl no se asociaron a la infección del mismo. Los gérmenes prevalentes fueron Escherichia coli y estreptococos del grupo viridans, cada uno aislado en 5 ocasiones. Los aislamientos fueron polimicrobianos en 5 de nueve casos (55.5%. La mortalidad por ascitis infectada fue de 27.3% (3/11 casos comparable con la debida a causas diferentes (29.4% en individuos con ascitis sin infección (5/17. Se concluye que la infección del líquido ascítico es una complicación frecuente en los pacientes cirróticos descompensados. La fiebre y el dolor abdominal son los signos clínicos cardinales de la infección. El aislamiento frecuente de E

  4. Properties and genomic analysis of Lactococcus garvieae lysogenic bacteriophage PLgT-1, a new member of Siphoviridae, with homology to Lactococcus lactis phages.

    Science.gov (United States)

    Hoai, Truong Dinh; Nishiki, Issei; Yoshida, Terutoyo

    2016-08-15

    The lysogenic phage PLgT-1 is highly prevalent in Lactococcus garvieae, which is a serious bacterial pathogen in marine fish. Therefore, information regarding this phage is one of the key factors to predict the evolution of this bacterium. However, many properties of this phage, its complete genome sequence, and its relationship with other viral communities has not been investigated to date. Here, we demonstrated that the phage PLgT-1 was not only induced by an induction agent (Mitomycin C), but could be released frequently during cell division in a nutrient-rich environment or in natural seawater. Integration of PLgT-1 into non-lysogenic bacteria via transduction changed the genotype, resulting in the diversification of L. garvieae. The complete DNA sequence of PLgT-1 was also determined. This phage has a dsDNA genome of 40,273bp with 66 open reading frames (ORFs). Of these, the biological functions of 24 ORFs could be predicted but those of 42 ORFs are unknown. Thus, PLgT-1 is a novel phage with several novel proteins encoded in its genome. The strict MegaBLAST search program for the PLgT-1 genome revealed that this phage had no similarities with other previously investigated phages specific to L. garvieae (WP-2 and GE1). Notably, PLgT-1 was relatively homologous with several phages of Lactococcus lactis and 17 of the 24 predicted proteins encoded in PLgT-1 were homologous with the deduced proteins of various phages from these dairy bacteria. Comparative genome analysis revealed that the L. garvieae phage PLgT-1 was most closely related to the L. lactis phage TP712. However, they differed from each other in genome size and gene arrangement. The results obtained in this study suggest that the lysogenic phage PLgT-1 is a new member of the family Siphoviridae and has been involved in horizontal gene exchange with microbial communities, especially with L. lactis and its phages. PMID:27234995

  5. Avaliação da rinussinusite bacteriana aguda em pacientes asmáticos com base em parâmetros clínicos, exame otorrinolaringológico e estudo de imagem Evaluation of acute bacterial rhinosinusitis in asthma patients based on clinical parameters and imaging studies, together with ear, nose and throat examination

    Directory of Open Access Journals (Sweden)

    Alecsandra Calil Moises Faure

    2008-06-01

    Full Text Available OBJETIVO: Avaliar os seios paranasais em pacientes com asma estável ou asma aguda para determinar a prevalência de rinossinusite bacteriana aguda. MÉTODOS: Estudo transversal incluindo 30 pacientes com asma aguda (73% do sexo feminino tratados na sala de emergência e 30 pacientes com asma estável (80% do sexo feminino regularmente acompanhados em ambulatório. Todos os pacientes responderam a um questionário sobre sinais e sintomas respiratórios e foram submetidos a exame otorrinolaringológico e a radiograma e tomografia computadorizada de seios paranasais. RESULTADOS: Com base no diagnóstico clínico, a prevalência de rinossinusite bacteriana aguda foi de 40% nos pacientes com asma aguda e de 3% nos com asma estável. O exame otorrinolaringológico e os exames de imagem isoladamente não foram úteis para a confirmação diagnóstica. CONCLUSÕES: O exame otorrinolaringológico e o radiograma e a tomografia de seios paranasais por si só não foram úteis para o diagnóstico de rinossinusite bacteriana aguda. Nossos resultados confirmam a evidência de que o diagnóstico clínico de rinossinusite aguda deve ser dado com cautela.OBJECTIVE: To evaluate paranasal sinuses in patients with stable or acute asthma in order to determine the prevalence of acute bacterial rhinosinusitis. METHODS: A cross-sectional study including 30 patients with acute asthma (73% females treated in the emergency room and 30 patients with stable asthma (80% females regularly monitored as outpatients. All patients completed a questionnaire on respiratory signs and symptoms and were submitted to ear, nose and throat (ENT examination, as well as to X-ray and computed tomography (CT imaging of the sinuses. RESULTS: Based on the clinical diagnosis, the prevalence of acute bacterial rhinosinusitis was 40% in the patients with acute asthma and 3% in those with stable asthma. The ENT examination findings and the imaging findings in isolation were not useful to confirm

  6. Bactérias endofíticas no controle e inibição in vitro de Pseudomonas syringae pv tomato, agente da pinta bacteriana do tomateiro Control with endophytic bacteria and in vitro inhibition of Pseudomonas syringae pv tomato, agent of bacterial speck of tomato

    Directory of Open Access Journals (Sweden)

    Juliana Resende Campos Silva

    2008-08-01

    Full Text Available Para avaliar o potencial de 53 isolados de bactérias endofíticas no controle da pinta bacteriana do tomateiro (Lycopersicum esculentum Mill., realizaram-se seleções massais em casa-de-vegetação e a seguir foi avaliado, in vitro, o antagonismo desses isolados sobre a bactéria desafiante Pseudomonas syringae pv. tomato (Pst. A inoculação das bactérias endofíticas foi feita por microbiolização das sementes de tomate cv. Santa Clara e da desafiante (Pst por pulverização. Aos 7, 14 e 21 dias após a inoculação da Pst, foram realizadas as avaliações da severidade da pinta bacteriana, bem como da altura das plantas. As espécies e os isolados bacterianos mais eficazes na redução da severidade da pinta bacteriana foram: Acinetobacter johnsonii (isolado 10, Bacillus pumilus (isolados 3, 12, 20, 39, 51, Paenibacillus macerans (isolados 37 e 47, PIM 11, Bacillus sphaericus (isolado 45, B. amyloliquefaciens (isolado 50, TOM 2, TOM 24 e Staphylococcus aureus (isolado 18. Mais de 50% dos isolados eficazes na redução da severidade foram da espécie Bacillus pumilus. Das espécies endofíticas mais eficazes na redução da severidade da pinta bacteriana, Bacillus pumilus e B. amyloliquefaciens inibiram também o crescimento da Pst in vitro.Vários dos isolados promoveram também o crescimento das plantas.To asses the potential of fifty three isolates of endophytic bacteria on the control of Pseudomonas syringae pv. tomato (Pst in tomato (Lycopersicum esculentum Mill., several screening were done in greenhouse followed by in vitro studies on antagonism of those isolates to Pst. The inoculation of endophytic bacteria was done by microbiolization of tomato cv Santa Clara seeds. The challenging bacterium (Pst inoculation was done by spraying. At 7, 14 and 21 days after Pst inoculation the assessment of bacterial speck severity was done, and height of plants was also measured. The most efficient endophytic species and isolates in reducing

  7. Atividade enzimática e perfil da comunidade bacteriana em solo submetido à solarização e biofumigação Enzymatic activity and bacterial community profile in soil under solarization e biofumigation

    Directory of Open Access Journals (Sweden)

    Samuel Ribeiro Passos

    2008-07-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito da solarização e da biofumigação sobre a comunidade microbiana do solo, por meio da atividade da enzima beta-glicosidase e do perfil do 16S rDNA, determinado com PCR-DGGE. A solarização do solo, com cobertura de plástico, foi feita por períodos de dois, quatro e seis meses, e a biofumigação foi realizada pela incorporação de 2 e 5% (v/v de cama-de-frango ao solo. Logo após a retirada da cobertura de plástico e aos 30 dias após a remoção, a atividade da beta-glicosidase foi menor em relação ao tratamento não solarizado. Aos 60 dias, não foram mais observadas diferenças entre os tratamentos. A adição de cama-de-frango a 5% estimulou a atividade da beta-glicosidase. O perfil da estrutura da comunidade bacteriana foi influenciado pelo tempo de solarização, independentemente da época da retirada da cobertura de plástico. Não foi observado efeito da adição de cama-de-frango ao solo, no perfil da comunidade. A solarização afeta a atividade da beta-glicosidase, mas esses efeitos não são mais detectáveis após 60 dias da retirada da cobertura de plástico, diferentemente do que foi observado em relação à estrutura da comunidade bacteriana por PCR-DGGE. A biofumigação estimula a atividade da beta-glicosidase, mas não afeta o perfil da comunidade microbiana.The objective of this work was to evaluate the effects of solarization and biofumigation on the soil microbial communities, by means of beta-glucosidase activity and 16S rDNA PCR-DGGE analyses. Solarization with a plastic covering of the soil took place over two, four and six months, and the soils were biofumigated by the addition of 2 and 5% (v/v of chicken litter to the soil. Right after the plastic cover removal and after 30 days, beta-glucosidase was lower than in the nonsolarizated control. After 60 days, there were no longer significant differences in beta-glucosidase activity between treatments. The addition

  8. Comparative effect of Pediococcus acidilactici and Lactococcus lactis on growth performance, survival and enzyme activity of western white leg shrimp (Litopenaeus vannamei)

    OpenAIRE

    Ahmadi, Sara

    2014-01-01

    This study was done in Shahid Kiani Marine Aquaculture Development Center, Choebde, Abadan in order to evaluate the effects of Pediiococcus acidilactici, Lactococcus lactis and vitamin C on growth performance, survival, enzymatic activities and immune responses of L. vannamei during three months. Treatments were included control group, Pediiococcus and Lactococcus treatments which fed with diet containing 1×10P9P cfu gP_1P bacteria and vitamin C. At the end of the experiment, the growth facto...

  9. Pneumonia associada à ventilação mecânica: impacto da multirresistência bacteriana na morbidade e mortalidade Ventilator-associated pneumonia: impact of bacterial multidrug-resistance on morbidity and mortality

    Directory of Open Access Journals (Sweden)

    Paulo José Zimermann Teixeira

    2004-12-01

    Full Text Available INTRODUÇÃO: A pneumonia associada à ventilação mecânica é a infecção hospitalar mais comum nas unidades de terapia intensiva. OBJETIVO: Determinar o impacto da multirresistência dos microorganismos na morbidade e mortalidade dos pacientes com pneumonia associada à ventilação mecânica. MÉTODO: Estudo de coorte retrospectivo. Em 40 meses consecutivos, 91 pacientes sob ventilação mecânica tiveram o diagnóstico de pneumonia. Os casos foram divididos entre causados por microorganismo multirresistente e causados por microorganismo sensível à antibioticoterapia. RESULTADOS: Pneumonia foi causada por microorganismo multirresistente em 75 casos (82,4% e por microorganismo sensível 16 (17,6% deles. As características clínicas e epidemiológicas não foram estatisticamente diferentes entre os grupos. O Staphylococcus aureus foi responsável por 27,5% dos episódios de pneumonia associada à ventilação mecânica e a Pseudomonas aeruginosa por 17,6%. A doença foi de início recente em 33 pacientes (36,3% e de início tardio em 58 deles (63,7%. Os tempos de ventilação mecânica, de internação em unidade de terapia intensiva e de internação hospitalar total não diferiram. O tratamento empírico foi considerado inadequado em 42 pacientes com pneumonia por microorganismo multirresistente (56% e em 4 com pneumonia por microorganismo sensível (25% (p = 0,02. Óbito ocorreu em 46 pacientes com a pneumonia por microorganismo multirresistente (61,3%, e em 4 daqueles com pneumonia por microorganismo sensível (25% (p = 0,008. CONCLUSÃO: A multirresistência bacteriana não determinou nenhum impacto na morbidade, mas esteve associada à maior mortalidade.BACKGROUND: Ventilator-associated pneumonia is the most common nosocomial infection occurring in intensive care units. OBJECTIVE: To determinate the impact of multidrug-resistant bacteria on morbidity and mortality in patients with ventilator-associated pneumonia. METHOD

  10. ClpE from Lactococcus lactis promotes repression of CtsR-dependent gene expression

    DEFF Research Database (Denmark)

    Varmanen, P.; Vogensen, F.K.; Hammer, Karin;

    2003-01-01

    The heat shock response in bacterial cells is characterized by rapid induction of heat shock protein expression, followed by an adaptation period during which heat shock protein synthesis decreases to a new steady-state level. In this study we found that after a shift to a high temperature the Clp...... ATPase (ClpE) in Lactococcus lactis is required for such a decrease in expression of a gene negatively regulated by the heat shock regulator (CtsR). Northern blot analysis showed that while a shift to a high temperature in wild-type cells resulted in a temporal increase followed by a decrease in......R homologue in Bacillus subtilis. Thus, our data point to a regulatory role of ClpE in turning off clpP gene expression following temporal heat shock induction, and we propose that this effect is mediated through CtsR....

  11. Analysis of heat shock gene expression in Lactococcus lactis MG1363

    DEFF Research Database (Denmark)

    Arnau, José; Sørensen, Kim; Appel, Karen Fuglede;

    1996-01-01

    The induction of the heat shock response in Lactococcus lactis subsp. cremoris strain MG1363 was analysed at the RNA level using a novel RNA isolation procedure to prevent degradation. Cloning of the dnaJ and groEL homologous was carried out. Nothern blot analysis showed a similar induction pattern...... heat shock response in L. lactis MG1363 is presented. A gene located downstream of the dnaK operon in strain MG1363, named orf4, was shown not to be regulated by heat shock......., although maximum induction was observed earlier for orf1 and grpE. Novel transcript sizes were detected in heat-shocked cells. The induction kinetics observed for ftsH suggested a different regulation for this gene. Experimental evidence for a prenounced transcriptional regulation being involved in the...

  12. Bacteriocinogenic Lactococcus lactis subsp. lactis DF04Mi isolated from goat milk: characterization of the bacteriocin.

    Science.gov (United States)

    Furtado, Danielle N; Todorov, Svetoslav D; Landgraf, Mariza; Destro, Maria T; Franco, Bernadette D G M

    2014-01-01

    Lactic acid bacteria capable of producing bacteriocins and presenting probiotic potential open innovative technological applications in the dairy industry. In this study, a bacteriocinogenic strain (Lactococcus lactis subsp. lactis DF4Mi) was isolated from goat milk, and studied for its antimicrobial activity. The bacteriocin presented a broad spectrum of activity, was sensitive to proteolytic enzymes, resistant to heat and pH extremes, and not affected by the presence of SDS, Tween 20, Tween 80, EDTA or NaCl. Bacteriocin production was dependent on the components of the culture media, especially nitrogen source and salts. When tested by PCR, the bacteriocin gene presented 100% homology to nisin Z gene. These properties indicate that this L. lactis subsp. lactis DF4Mi can be used for enhancement of dairy foods safety and quality. PMID:25763065

  13. Bacteriocinogenic Lactococcus lactis subsp. lactis DF04Mi isolated from goat milk: characterization of the bacteriocin

    Directory of Open Access Journals (Sweden)

    Danielle N. Furtado

    2014-12-01

    Full Text Available Lactic acid bacteria capable of producing bacteriocins and presenting probiotic potential open innovative technological applications in the dairy industry. In this study, a bacteriocinogenic strain (Lactococcus lactis subsp. lactis DF4Mi was isolated from goat milk, and studied for its antimicrobial activity. The bacteriocin presented a broad spectrum of activity, was sensitive to proteolytic enzymes, resistant to heat and pH extremes, and not affected by the presence of SDS, Tween 20, Tween 80, EDTA or NaCl. Bacteriocin production was dependent on the components of the culture media, especially nitrogen source and salts. When tested by PCR, the bacteriocin gene presented 100% homology to nisin Z gene. These properties indicate that this L. lactis subsp. lactis DF4Mi can be used for enhancement of dairy foods safety and quality.

  14. Production of recombinant peanut allergen Ara h 2 using Lactococcus lactis

    DEFF Research Database (Denmark)

    Glenting, J.; Poulsen, Lars K.; Kato, K.; Madsen, S.M.; Frøkiær, Hanne; Wendt, C.; Sorensen, H.W.

    2007-01-01

    Background: Natural allergen sources can supply large quantities of authentic allergen mixtures for use as immunotherapeutics. However, such extracts are complex, difficult to define, vary from batch to batch, which may lead to unpredictable efficacy and/ or unacceptable levels of side effects. The...... use of recombinant expression systems for allergen production can alleviate some of these issues. Several allergens have been tested in high- level expression systems and in most cases show immunereactivity comparable to their natural counterparts. The gram positive lactic acid bacterium Lactococcus...... lactis is an attractive microorganism for use in the production of protein therapeutics. L. lactis is considered food grade, free of endotoxins, and is able to secrete the heterologous product together with few other native proteins. Hypersensitivity to peanut represents a serious allergic problem. Some...

  15. Oxidative Stress at High Temperatures in Lactococcus lactis Due to an Insufficient Supply of Riboflavin

    DEFF Research Database (Denmark)

    Shen, Jing; Solem, Christian; Jensen, Peter Ruhdal;

    2013-01-01

    Lactococcus lactis MG1363 was found to be unable to grow at temperatures above 37°C in a defined medium without riboflavin, and the cause was identified to be dissolved oxygen introduced during preparation of the medium. At 30°C, growth was unaffected by dissolved oxygen and oxygen was consumed...... riboflavin to the medium, it was possible to improve growth and oxygen consumption at 37°C, and this also normalized the [ATP]-to-[ADP] ratio. A codon-optimized redox-sensitive green fluorescent protein (GFP) was introduced into L. lactis and revealed a more oxidized cytoplasm at 37°C than at 30°C. These...... results indicate that L. lactis suffers from heat-induced oxidative stress at increased temperatures. A decrease in intracellular flavin adenine dinucleotide (FAD), which is derived from riboflavin, was observed with increasing growth temperature, but the presence of riboflavin made the decrease smaller...

  16. The pyrimidine operon pyrRPB-carA from Lactococcus lactis

    DEFF Research Database (Denmark)

    Martinussen, Jan; Schallert, J.; Andersen, Birgit;

    2001-01-01

    The four genes pyrR, pyrP, pyrB, and carA were found to constitute an operon in Lactococcus lactis subsp, lactis MG1363. The functions of the different genes were established by mutational analysis. The first gene in the operon is the pyrimidine regulatory gene, pyrR, which is responsible for the...... regulation of the expression of the pyrimidine biosynthetic genes leading to UMP formation. The second gene encodes a membrane-bound high-affinity uracil permease, required for utilization of exogenous uracil. The last two genes in the operon, pyrB and carA, encode pyrimidine biosynthetic enzymes; aspartate...... transcarbamoylase (pyrB) is the second enzyme in the pathway, whereas carbamoyl-phosphate synthetase subunit A (carA) is the small subunit of a heterodimeric enzyme, catalyzing the formation of carbamoyl phosphate. The carA gene product is shown to be required for both pyrimidine and arginine biosynthesis. The...

  17. Angiotensin-converting enzyme inhibitory activity of milk fermented by wild and industrial Lactococcus lactis strains.

    Science.gov (United States)

    Rodríguez-Figueroa, J C; Reyes-Díaz, R; González-Córdova, A F; Troncoso-Rojas, R; Vargas-Arispuro, I; Vallejo-Cordoba, B

    2010-11-01

    Angiotensin I-converting enzyme inhibitory (ACEI) activity was evaluated and compared in milk fermented by wild and commercial starter culture Lactococcus lactis strains after 48 h of incubation. The highest ACEI activities were found in WSE from milk inoculated with wild L. lactis strains isolated from artisanal dairy products and commercial starter cultures. On the other hand, the lowest ACEI activities were found in WSE from milk inoculated with wild strains isolated from vegetables. Moreover, the IC(50) values (concentration that inhibits 50% activity) of WSE from artisanal dairy products were the lowest, indicating that these fractions were the most effective in inhibiting 50% of ACE activity. In fact, a strain isolated from artisanal cheese presented the lowest IC(50) (13 μg/mL). Thus, it appears that wild L. lactis strains isolated from artisanal dairy products and commercial starter cultures showed good potential for the production of fermented dairy products with ACEI properties. PMID:20965317

  18. Physiochemical parameters optimization for enhanced nisin production by Lactococcus lactis (MTCC 440

    Directory of Open Access Journals (Sweden)

    Puspadhwaja Mall

    2010-02-01

    Full Text Available The influence of various physiochemical parameters on the growth of Lactococcus lactis sub sp. lactis MTCC 440 was studied at shake flask level for 20 h. Media optimization (MRS broth was studied to achieve enhanced growth of the organism and also nisin production. Bioassay of nisin was done with agar diffusion method using Streptococcus agalactae NCIM 2401 as indicator strain. MRS broth (6%, w/v with 0.15μg/ml of nisin supplemented with 0.5% (v/v skimmed milk was found to be the best for nisin production as well as for growth of L lactis. The production of nisin was strongly influenced by the presence of skimmed milk and nisin in MRS broth. The production of nisin was affected by the physical parameters and maximum nisin production was at 30(0C while the optimal temperature for biomass production was 37(0C.

  19. Characterization of Lactococcus lactis mutants with improved performance at high temperatures and potential dairy applications

    DEFF Research Database (Denmark)

    Chen, Jun

    Lactococcus lactis (L. lactis) is a Gram-positive mesophile, which has considerable importance in the dairy industry for production of cheese and butter milk, and which carries the “GRAS” (generally recognized as safe) designation. Temperature has a great impact on dairy fermentation processes...... latter because the increased energy consumption at high temperatures potentially could stimulate glycolysis. However in many cases the fitness is affected and mostly negative effects on productivity are observed. In this study, the non-GMO approach, experimental adaptation, was employed for isolating...... lactate production rates when compared to MG1363 at high temperatures. Whole genome re-sequencing identified 13 SNPs, one DIP and one large deletion in TM29, and additional sequencing of the isolated intermediates indicated dynamic accumulation of mutations with rising fitness in a temporal order. DNA...

  20. Increased biomass yield of Lactococcus lactis during energetically limited growth and respiratory conditions

    DEFF Research Database (Denmark)

    Købmann, Brian Jensen; Blank, Lars Mathias; Solem, Christian;

    2008-01-01

    subtracting these contributions, approx. 0.3 mol of ATP/mol of glucose remained unaccounted for. A similar response to respiratory conditions (0.2 mol of ATP/mol of glucose) was observed in a mutant that had a decreased glucose uptake rate during growth on glucose caused by disruption of the PTSmannose......Lactococcus lactis is known to be capable of respiration under aerobic conditions in the presence of haemin. In the present study the effect of respiration on ATP production during growth on different sugars was examined. With glucose as the sole carbon source, respiratory conditions in L. lactis...... MG1363 resulted in only a minor increase, 21%, in biomass yield. Since ATP production through substrate-level phosphorylation was essentially identical with and without respiration, the increased biomass yield was a result of energy-saving under respiratory conditions estimated to be 0.4 mol of ATP...

  1. Bacterial pneumonia following bone marrow transplantation: HRCT findings Achados de TCAR nas pneumonias bacterianas após transplante de medula óssea

    Directory of Open Access Journals (Sweden)

    Luiz Otávio de Mattos Coelho

    2009-05-01

    Full Text Available OBJECTIVE: To describe HRCT findings in patients with bacterial pneumonia following bone marrow transplantation (BMT. METHODS: This was a retrospective study involving 30 patients diagnosed with bacterial pneumonia in whom HRCT of the chest was performed within 24 h after the onset of symptoms and the diagnosis was confirmed, based on a positive culture of sputum or bronchial aspirate, together with a positive pleural fluid or blood culture, within one week after symptom onset. There were 20 male patients and 10 female patients. The median age was 21 years (range, 1-41 years. The BMT had been performed for the treatment of the following: chronic myeloid leukemia, in 14 cases; severe aplastic anemia, in 6; acute myeloid leukemia, in 4; Fanconi's anemia, in 3; and acute lymphocytic leukemia, in 3. Two radiologists analyzed the HRCT scans and reached their final decisions by consensus. RESULTS: The most common HRCT findings were air-space consolidation (in 60%, small centrilobular nodules (in 50%, ground-glass opacities (in 40%, bronchial wall thickening (in 20%, large nodules (in 20%, pleural lesions (in 16.7% and tree-in-bud opacities (in 10%. The pulmonary lesions were distributed in the central and peripheral areas in 15 patients, whereas they were exclusively peripheral in 11. Lesions were located in the lower and middle lobes of the lung in 22 and 20 patients, respectively. CONCLUSIONS: The most common HRCT findings in our patient sample were air-space consolidation, small centrilobular nodules and ground-glass opacities, most often in the central and peripheral regions of the middle and lower lung zones.OBJETIVO: Descrever os achados de TCAR em pacientes com pneumonia bacteriana após transplante de medula óssea (TMO. MÉTODOS: Estudo retrospectivo com 30 pacientes diagnosticados com pneumonia bacteriana, documentada com TCAR do tórax realizada em até 24 h do início dos sintomas, e com diagnóstico comprovado com base em cultura positiva

  2. Sensibilidade a cobre, estreptomicina e oxitetraciclina em Xanthomonas spp. associadas à mancha-bacteriana do tomate para processamento industrial

    Directory of Open Access Journals (Sweden)

    Quezado-Duval Alice Maria

    2003-01-01

    Full Text Available Apesar de amplamente empregados em lavouras de tomate para processamento industrial no Brasil, fungicidas cúpricos e antibióticos registrados para uso agrícola nem sempre resultam em controle eficiente das bacterioses que afetam a cultura. O aparecimento de estirpes resistentes é uma das causas dessa baixa eficiência. Avaliou-se, in vitro, a sensibilidade a cobre, estreptomicina e oxitetraciclina de 389 isolados de Xanthomonas spp. associadas à mancha-bacteriana do tomateiro, sendo 92 de X. axonopodis pv. vesicatoria (60 do grupo "A"/raça T1 e 32 do "C"/raça T3, 93 de X. vesicatoria (grupo "B"/raça T2 e 204 de X. gardneri (grupo "D"/raça T2. Os isolados foram obtidos de plantas doentes em campos comerciais de tomate para processamento industrial nos estados de Goiás, Minas Gerais, Pernambuco e Bahia, nos anos de 1995 a 1998 e em 2000. Alíquotas de 5 ml de suspensões bacterianas foram depositadas em meio Nutriente-Ágar suplementado com sulfato de cobre, nas concentrações de 50 e 200 µg/ml; sulfato de estreptomicina, a 25 e 200 µg/ml e cloridrato de oxitetraciclina, a 25 µg/ml. Nenhum isolado foi resistente a oxitetraciclina, como também nenhum foi resistente ao cobre na concentração de 200 µg/ml do sulfato de cobre. No entanto, houve diferença entre isolados quanto à sensibilidade ao sulfato de cobre na concentração de 50 µg/ml e ao sulfato de estreptomicina nas duas concentrações empregadas. As freqüências de isolados de X. gardneri, X. axonopodis pv. vesicatoria (grupos "A" e "C" e X. vesicatoria resistentes à estreptomicina (25 µg/ml do produto usado foram, respectivamente, 98%, 38% e 2%, ao passo que, ao cobre, foram, respectivamente, 48%, 4% e 74%. Todos os isolados do grupo "C" foram sensíveis à estreptomicina e 97% sensíveis ao cobre.

  3. Tumorogênese em plantas causadas por espécies de Agrobacterium Tumorigenesis in plants induced by species of Agrobacterium

    OpenAIRE

    Reginaldo da Silva Romeiro; José Roberto Vieira Júnior; Sérgio Hermínio Brommonschenkel

    2007-01-01

    Tumores - sintomas hiperplásicos em plantas - incitados por espécies de Agrobacterium sp. sempre exerceram fascínio sobre fitopatologistas desde o início do Século XX, quando Erwin Smith e colaboradores demonstraram serem eles de etiologia bacteriana. No início, imaginava-se que os tumores eram decorrentes de alterações hormonais na planta provocadas pela bactéria. Contudo, até recentemente, a microbiologia e a biologia molecular não eram suficientemente avançadas para que os cientistas pudes...

  4. Uso de corticosteroide na infecção grave de origem peritoneal por bacterias gram-positivas ou gram-negativas, experimentalmente induzida em camundongos

    OpenAIRE

    Fadel, Marcus Vinicius Telles

    2012-01-01

    Resumo: Objetivos: O principal objetivo deste trabalho foi avaliar o papel dos corticosteróides em um modelo experimental de infecção. A hipótese era que, se tais drogas são capazes de inibir a produção e/ou efeito de diversos mediadores da sepse, elas poderiam exercer um efeito benéfico, contanto que sua administração fosse feita antes da liberação maciça de produtos da parede bacteriana (ocasionada, dentre outros fatores, por antibióticos), produtos estes encarados como responsáveis pela se...

  5. Receptores de la prostaglandina E2 implicados en los trastornos motores digestivos y la fiebre inducidos por endotoxinas en la oveja: Papel de los macrófagos

    OpenAIRE

    Gimeno Soriano, Aurora; Plaza Carrión, Miguel Ángel; Castro López, Marta

    2014-01-01

    El objetivo general de este trabajo ha sido el estudio de los mecanismos fisiopatológicos implicados en la fiebre y en las alteraciones motoras digestivas inducidas por una endotoxina bacteriana: el lipopolisacárido (LPS) de Escherichia coli en la oveja. Se ha estudiado la participación de los receptores de la prostaglandina (PG) E2 (EP1, EP2, EP3 y EP4), los tipos celulares que están implicados en la síntesis y liberación de PGs en el hipotálamo y bulbo raquídeo y la posible participación de...

  6. Valorización de residuos agroindustriales para la obtención de liquenisina por Bacillus licheniformis AL 1.1

    OpenAIRE

    Coronel León, Jonathan

    2015-01-01

    [spa] La cepa bacteriana AL 1.1 se aisló de una muestra de suelo de la isla Decepción del continente Antártico. Inicialmente se observó que dicha cepa tenía la capacidad de reducir la tensión superficial del medio, por lo que fue seleccionada para realizar la presente tesis doctoral. Después de confirmar la identificación del aislado como un Bacillus licheniformis se caracterizó el biotensioactivo (BT) producido como liquenisina (LchAL1.1). El estudio de las propiedades físico-químicas de la ...

  7. Microbiota of Minas cheese as influenced by the nisin producer Lactococcus lactis subsp. lactis GLc05.

    Science.gov (United States)

    Perin, Luana Martins; Dal Bello, Barbara; Belviso, Simona; Zeppa, Giuseppe; de Carvalho, Antônio Fernandes; Cocolin, Luca; Nero, Luís Augusto

    2015-12-01

    Minas cheese is a popular dairy product in Brazil that is traditionally produced using raw or pasteurized cow milk. This study proposed an alternative production of Minas cheese using raw goat milk added of a nisin producer Lactococcus lactis subsp. lactis GLc05. An in situ investigation was carried on to evaluate the interactions between the L. lactis subsp. lactis GLc05 and the autochthonous microbiota of a Minas cheese during the ripening; production of biogenic amines (BAs) was assessed as a safety aspect. Minas cheese was produced in two treatments (A, by adding L. lactis subsp. lactis GLc05, and B, without adding this strain), in three independent repetitions (R1, R2, and R3). Culture dependent (direct plating) and independent (rep-PCR and PCR-DGGE) methods were employed to characterize the microbiota and to assess the possible interferences caused by L. lactis subsp. lactis GLc05. BA amounts were measured using HPLC. A significant decrease in coagulase-positive cocci was observed in the cheeses produced by adding L. lactis subsp. lactis GLc05 (cheese A). The rep-PCR and PCR-DGGE highlighted the differences in the microbiota of both cheeses, separating them into two different clusters. Lactococcus sp. was found as the main microorganism in both cheeses, and the microbiota of cheese A presented a higher number of species. High concentrations of tyramine were found in both cheeses and, at specific ripening times, the BA amounts in cheese B were significantly higher than in cheese A (p<0.05). The interaction of nisin producer L. lactis subsp. lactis GLc05 was demonstrated in situ, by demonstration of its influence in the complex microbiota naturally present in a raw goat milk cheese and by controlling the growth of coagulase-positive cocci. L. lactis subsp. lactis GLc05 influenced also the production of BA determining that their amounts in the cheeses were maintained at acceptable levels for human consumption. PMID:26310130

  8. Efeito da solarização e biofumigação na incidência da murcha bacteriana em tomateiro no campo Effect of soil solarization and biofumigation on tomato bacterial wilt incidence

    Directory of Open Access Journals (Sweden)

    Mírian J Baptista

    2006-06-01

    Full Text Available As doenças causadas por fitopatógenos do solo causam elevados prejuízos na maioria das espécies olerícolas. A solarização e a biofumigação são estratégias para controle destes organismos com potencial para aplicação em sistemas de manejo integrado. Neste trabalho, foi conduzido um experimento na Embrapa Hortaliças, Brasília - DF, para avaliar os efeitos da adição de resíduos orgânicos (biofumigação e da solarização na incidência natural da murcha-bacteriana do tomateiro, causada por Ralstonia solanacearum, nas características químicas do solo e na ocorrência de plantas invasoras. Os tratamentos avaliados foram adição de resíduos de brássicas (2% v/v, cama de frango (2% v/v, tratamento com brometo de metila e solo sem tratamento, todos solarizados ou não solarizados. Após a solarização, foram coletadas amostras de solo para determinação de suas características químicas e do banco de sementes de plantas invasoras. A solarização do solo provocou redução significativa no pH e nos teores de B e Zn. A adição de matéria orgânica ocasionou aumentos nos teores de Ca, K, e Na e reduziu os teores de Al. O banco de sementes de plantas invasoras, principalmente monocotiledôneas foi também reduzido significativamente. O tratamento com brometo de metila e a adição de cama de frango reduziram significativamente a incidência de murcha-bacteriana.Soilborne plant pathogens cause heavy losses to many vegetable crops. Solarization and organic residues amendments have been evaluated as disease control strategies with good perspectives for aplication in integrated management. Field experiments were carried out at Embrapa Hortaliças, Brasília-DF, to evaluate the effects of amending soil with organic residues (biofumigation and solarization on the incidence of bacterial wilt (Ralstonia solanacearum on tomato, on soil chemical characteristics, and weed control. Amendments with brassica residues (2%, chicken litter

  9. Raças de Xanthomonas spp. associadas à mancha-bacteriana em tomate para processamento industrial no Brasil Races of Xanthomonas spp. associated to bacterial spot in processing tomatoes in Brazil

    Directory of Open Access Journals (Sweden)

    Alice Maria Quezado-Duval

    2004-03-01

    Full Text Available A grande diversidade genética dos agentes causadores da mancha-bacteriana dificulta sobremaneira o desenvolvimento de variedades de pimentão e tomate com resistência durável. Setenta e dois isolados de Xanthomonas spp. provenientes de campos comerciais de tomate para processamento industrial dos estados de Goiás, Minas Gerais, Pernambuco e Bahia foram classificados em raças com base nas reações de genótipos diferenciais de tomateiro (Walter, Hawaii 7998 e NIL 216 e de Capsicum (ECW [Early Calwonder], ECW-10R, ECW-20R, ECW-30R e PI235047. As plantas foram inoculadas no estádio de três a cinco folhas verdadeiras por infiltração de suspensão bacteriana (5 ´ 10(8 UFC/ml na superfície abaxial da folha. Em seguida, foram mantidas em câmara de crescimento em fotoperíodo de 12 h/12 h (luz/escuro a 28ºC. A reação de hipersensibilidade foi observada até 36 horas após a inoculação, dependendo do genótipo da hospedeira. Foram identificadas as raças T1P2, T1P8 e T3 em X. axonopodis pv. vesicatoria; a raça T2 em X. vesicatoria; e as raças T2P7 e T2P8 em X. gardneri. A presença dos genes avrRxv e avrXv3 nos isolados que causaram reação de hipersensibilidade em 'Hawaii 7998' (raça T1 e 'NIL 216' (raça T3, respectivamente, foi confirmada por reação em cadeia da polimerase (PCR usando iniciadores específicos. Este é o primeiro relato da ocorrência no Brasil das raças T3, T1P8, T2P7 e T2P8.The great genetic diversity of the causal agents of bacterial spot is the main problem to the development of tomato and pepper varieties with durable resistance. Seventy two strains of Xanthomonas spp. collected from commercial fields of processing tomatoes in the states of Goiás, Minas Gerais, Pernambuco, and Bahia were classified in races according to their reactions on differential genotypes of tomato (Walter, Hawaii 7998 and NIL 216 and Capsicum [ECW (Early Calwonder, ECW-10R, ECW-20R, ECW-30R and PI 235047]. Bacterial suspensions (5

  10. Produção e degradação in vitro de estruturas tubulares de celulose bacteriana

    Directory of Open Access Journals (Sweden)

    Vanessa A. Oliveira

    2013-01-01

    Full Text Available A necessidade de novos recursos em cardiologia tem direcionado a Engenharia de Tecidos ao desenvolvimento de vasos sanguíneos artificiais que atendam aos requisitos do organismo. Neste trabalho, estruturas tubulares de celulose bacteriana (CB foram produzidas e sua degradação in vitro foi avaliada. Através de microscopia eletrônica de varredura constatou-se que não houve alterações significativas na microestrutura e morfologia das fibras de CB após ensaios de degradação. Os ensaios de degradação em soluções fisiológicas PBS e salina revelaram uma degradação substancialmente baixa após 20 semanas. A baixa velocidade de degradação dos vasos é de grande importância, visto que o processo para a formação de novos vasos (angiogênese demanda tempo.

  11. Characterization of the Lactococcus lactis lactose operon promoter: contribution of flanking sequences and LacR repressor to promoter activity.

    OpenAIRE

    van Rooijen, R J; Gasson, M. J.; de Vos, W M

    1992-01-01

    We determined the location, activity, and regulation of the promoter of the Lactococcus lactis 8-kb lactose operon (lacABCDFEGX), which encodes the enzymes of the lactose phosphotransferase system and the tagatose 6-phosphate pathway. The lac promoter sequence corresponds closely to the consensus promoter described for gram-positive bacteria and is located in a back-to-back configuration with the promoter of the divergently transcribed lacR gene, which encodes the LacR repressor. The transcri...

  12. Transcriptional Regulation and Evolution of Lactose Genes in the Galactose-Lactose Operon of Lactococcus lactis NCDO2054

    OpenAIRE

    Vaughan, Elaine E.; Pridmore, R. David; Mollet, Beat

    1998-01-01

    The genetics of lactose utilization within the slow-lactose-fermenting Lactococcus lactis strain NCDO2054 was studied with respect to the organization, expression, and evolution of the lac genes. Initially the β-galactosidase gene (lacZ) was cloned by complementation of an Escherichia coli mutant on a 7-kb HpaI fragment. Nucleotide sequence analysis of the complete fragment revealed part of a gal-lac operon, and the genes were characterized by inactivation and complementation analyses and in ...

  13. Draft Genome Sequence of Lactococcus lactis subsp. lactis bv. diacetylactis CRL264, a Citrate-Fermenting Strain

    OpenAIRE

    Zuljan, Federico; Espariz, Martín; Blancato, Victor S.; Esteban, Luis; Alarcón, Sergio; Magni, Christian

    2016-01-01

    We report the draft genome sequence of Lactococcus lactis subsp. lactis bv. diacetylactis CRL264, a natural strain isolated from artisanal cheese from northwest Argentina. L. lactis subsp. lactis bv. diacetylactis is one of the most important microorganisms used as starter culture around the world. The CRL264 strain constitutes a model microorganism in the studies on the generation of aroma compounds (diacetyl, acetoin, and 2,3-butanediol) by lactic acid bacteria. Our genome analysis shows si...

  14. Bacteriocinogenic Lactococcus lactis subsp. lactis DF04Mi isolated from goat milk: Evaluation of the probiotic potential

    OpenAIRE

    Furtado, Danielle N.; Todorov, Svetoslav D.; Mariza Landgraf; Maria T. Destro; Franco, Bernadette D.G.M.

    2014-01-01

    Lactic acid bacteria capable of producing bacteriocins and presenting probiotic potential open innovative technological applications in the dairy industry. In this study, a bacteriocinogenic strain (Lactococcus lactis subsp. lactis DF4Mi) was isolated from goat milk, and studied for its probiotic potential. Lc. lactis DF4Mi was resistant to acidic pH and oxbile, presented co-aggregation with Listeria monocytogenes, and was not affected by several drugs from different generic groups, being sen...

  15. Expression of a Heterologous Glutamate Dehydrogenase Gene in Lactococcus lactis Highly Improves the Conversion of Amino Acids to Aroma Compounds

    OpenAIRE

    Rijnen, Liesbeth; Courtin, Pascal; Gripon, Jean-Claude; Yvon, Mireille

    2000-01-01

    The first step of amino acid degradation in lactococci is a transamination, which requires an α-keto acid as the amino group acceptor. We have previously shown that the level of available α-keto acid in semihard cheese is the first limiting factor for conversion of amino acids to aroma compounds, since aroma formation is greatly enhanced by adding α-ketoglutarate to cheese curd. In this study we introduced a heterologous catabolic glutamate dehydrogenase (GDH) gene into Lactococcus lactis so ...

  16. Molecular and Functional Analyses of the metC Gene of Lactococcus lactis, Encoding Cystathionine β-Lyase

    OpenAIRE

    Fernández, María; Doesburg, Wim van; Rutten, Ger A.M.; Marugg, Joey D.; Alting, Arno C.; van Kranenburg, Richard; Oscar P. Kuipers

    2000-01-01

    The enzymatic degradation of amino acids in cheese is believed to generate aroma compounds and therefore to be essential for flavor development. Cystathionine β-lyase (CBL) can convert cystathionine to homocysteine but is also able to catalyze an α,γ elimination. With methionine as a substrate, it produces volatile sulfur compounds which are important for flavor formation in Gouda cheese. The metC gene, which encodes CBL, was cloned from the Lactococcus lactis model strain MG1363 and from str...

  17. The protective effect of recombinant Lactococcus lactis oral vaccine on a Clostridium difficile-infected animal model

    OpenAIRE

    Yang, Xiao-qiang; Zhao, Ya-Gang; Chen, Xue-Qing; Jiang, Bo; SUN, DA-YONG

    2013-01-01

    Background Oral immunization with vaccines may be an effective strategy for prevention of Clostridium difficile infection (CDI). However, application of previously developed vaccines for preventing CDI has been limited due to various reasons. Here, we developed a recombinant Lactococcus lactis oral vaccine and evaluated its effect on a C. difficile-infected animal model established in golden hamsters in attempt to provide an alternative strategy for CDI prevention. Methods Recombinant L. lact...

  18. The carB Gene Encoding the Large Subunit of Carbamoylphosphate Synthetase from Lactococcus lactis Is Transcribed Monocistronically

    OpenAIRE

    Martinussen, Jan; Hammer, Karin

    1998-01-01

    The biosynthesis of carbamoylphosphate is catalyzed by the heterodimeric enzyme carbamoylphosphate synthetase. The genes encoding the two subunits of this enzyme in procaryotes are normally transcribed as an operon, but the gene encoding the large subunit (carB) in Lactococcus lactis is shown to be transcribed as an isolated unit. Carbamoylphosphate is a precursor in the biosynthesis of both pyrimidine nucleotides and arginine. By mutant analysis, L. lactis is shown to possess only one carB g...

  19. Secretion of TEM beta-lactamase with signal sequences isolated from the chromosome of Lactococcus lactis subsp. lactis.

    OpenAIRE

    Sibakov, M; Koivula, T; von Wright, A.; Palva, I

    1991-01-01

    With TEM beta-lactamase as a reporter gene, a set of expression-secretion-promoting fragments were isolated from the chromosome of Lactococcus lactis subsp. lactis. The fact that only translocated beta-lactamase renders cells resistant to ampicillin allowed direct ampicillin selection with an Escherichia coli vector (pKTH33). The clones showing the greatest ampicillin resistance were subcloned onto a replicon capable of replication in lactic acid bacteria (pVS2), and the nucleotide sequences ...

  20. Glucose Metabolism in Lactococcus lactis MG1363 under Different Aeration Conditions: Requirement of Acetate To Sustain Growth under Microaerobic Conditions

    OpenAIRE

    Nordkvist, Mikkel; Jensen, Niels Bang Siemsen; Villadsen, John

    2003-01-01

    Lactococcus lactis subsp. lactis MG1363 was grown in batch cultures on a defined medium with glucose as the energy source under different aeration conditions, namely, anaerobic conditions, aerobic conditions, and microaerobic conditions with a dissolved oxygen tension of 5% (when saturation with air was used as the reference). The maximum specific growth rate was high (0.78 to 0.91 h−1) under all aeration conditions but decreased with increasing aeration, and more than 90% of the glucose was ...

  1. Oral Treatment with Lactococcus lactis Expressing Staphylococcus hyicus Lipase Enhances Lipid Digestion in Pigs with Induced Pancreatic Insufficiency

    OpenAIRE

    Drouault, Sophie; Juste, Catherine; Marteau, Philippe; Renault, Pierre; Corthier, Gérard

    2002-01-01

    The Staphylococcus hyicus lip gene was cloned in Lactococcus lactis. Pancreatic insufficiency was induced by ligation of the pancreatic duct in pigs. In pigs who had undergone pancreatic ligation, the coefficient of fat absorption was higher after consumption of lipase-expressing L. lactis (91.9% ± 3.7%) than that after consumption of the inactive control strain (78.4% ± 2.4%).

  2. Intranasal Immunization with Recombinant Lactococcus lactis Secreting Murine Interleukin-12 Enhances Antigen-Specific Th1 Cytokine Production

    OpenAIRE

    Bermudez Humaran, Luis; Langella, Philippe; Cortes-Perez, Naima; Gruss, Alexandra; Tamez-Guerra, Reyes S; Oliveira, Sergio C.; Saucedo-Cardenas, Odila; Montes de Oca-Luna, Roberto; Le Loir, Yves

    2003-01-01

    Interleukin-12 (IL-12), a heterodimeric cytokine, plays an important role in cellular immunity to several bacterial, viral, and parasitic infections and has adjuvant activity when it is codelivered with DNA vaccines. IL-12 has also been used with success in cancer immunotherapy treatments. However, systemic IL-12 therapy has been limited by high levels of toxicity. We describe here inducible expression and secretion of IL-12 in the food-grade lactic acid bacterium Lactococcus lactis. IL-12 wa...

  3. Reassessing the Role of Staphylococcus aureus Clumping Factor and Fibronectin-Binding Protein by Expression in Lactococcus lactis

    OpenAIRE

    Que, Yok-Ai; François, Patrice; Haefliger, Jacques-Antoine; Entenza, José-Manuel; Vaudaux, Pierre; Moreillon, Philippe

    2001-01-01

    Since Staphylococcus aureus expresses multiple pathogenic factors, studying their individual roles in single-gene-knockout mutants is difficult. To circumvent this problem, S. aureus clumping factor A (clfA) and fibronectin-binding protein A (fnbA) genes were constitutively expressed in poorly pathogenic Lactococcus lactis using the recently described pOri23 vector. The recombinant organisms were tested in vitro for their adherence to immobilized fibrinogen and fibronectin and in vivo for the...

  4. Heterologous production of methionine-γ-lyase from brevibacterium linens in lactococcus lactis and formation of volatile sulfur compounds

    OpenAIRE

    Hanniffy, Sean; Philo, Mark; Peláez, Carmen; Gasson, M. J.; Requena, Teresa; Martínez-Cuesta, M. Carmen

    2009-01-01

    The conversion of methionine to volatile sulfur compounds (VSCs) is of great importance in flavor formation during cheese ripening and is the focus of biotechnological approaches toward flavor improvement. A synthetic mgl gene encoding methionine-γ-lyase (MGL) from Brevibacterium linens BL2 was cloned into a Lactococcus lactis expression plasmid under the control of the nisin-inducible promoter PnisA. When expressed in L. lactis and purified as a recombinant protein, MGL was shown to degrade ...

  5. Proton motive force-driven and ATP-dependent drug extrusion systems in multidrug-resistant Lactococcus lactis.

    OpenAIRE

    Bolhuis, H.; Molenaar, D.; POELARENDS, G; VANVEEN, HW; Poolman, B; Driessen, AJM; KONINGS, WN

    1994-01-01

    Three mutants of Lactococcus lactis subsp. lactis MG1363, termed Eth(R), Dau(R), and Rho(R), were selected for resistance to high concentrations of ethidium bromide, daunomycin, and rhodamine 6G, respectively. These mutants were found to be cross resistant to a number of structurally and functionally unrelated drugs, among which were typical substrates of the mammalian multidrug transporter (P-glycoprotein) such as daunomycin, quinine, actinomycin D, gramicidin D, and rhodamine 6G. The three ...

  6. Rhizosphere bacterial communities of potato cultivars evaluated through PCR-DGGE profiles Comunidades bacterianas associadas à rizosfera de cultivares de batata avaliadas por perfis de PCR-DGGE

    OpenAIRE

    Enderson Petrônio de Brito Ferreira; André Nepomuceno Dusi; Gustavo Ribeiro Xavier; Norma Gouvêa Rumjanek

    2008-01-01

    The objective of this work was to determine the shifts on the PCR-DGGE profiles of bacterial communities associated to the rhizosphere of potato cultivars, in order to generate baseline information for further studies of environmental risk assessment of genetically modified potato plants. A greenhouse experiment was carried out with five potato cultivars (Achat, Bintje, Agata, Monalisa and Asterix), cultivated in pots containing soil from an integrated system for agroecological production. Th...

  7. Uso de Extractos de Pseudomonas sp (PB11 para el Control de la Mancha Bacteriana en tomate (Solanum lycopersicum Use of Pseudomonas sp (PB11 Extracts for Control of Bacterial Speck in Tomatoes (Solanum lycopersicum

    Directory of Open Access Journals (Sweden)

    Guadalupe Oyoque

    2011-01-01

    Full Text Available Se ha usado extracto libre de células de la cepa patógena Pseudomonas sp (PB11 causante de la mancha bacteriana en tomate para control de la misma cepa en plantas de tomate (Solanum lycopersicum en condiciones de invernadero. El extracto libre de células de PB11 fue aplicado a plantas de un mes de edad. Después de cuatro días, las plantas fueron inoculadas con una suspensión de Pseudomonas sp PB11. La severidad de la infección fue determinada por el número de lesiones necróticas presentes en cada planta y expresada como porcentaje de daño total. El extracto permitió la disminución de la infección en más del 60 % (pCell free extract from a pathogenic strain of Pseudomonas sp (PB11 able to cause bacterial speck was prompted for protection against that disease in tomato plants (Solana lycopersicum under greenhouse conditions. This extract was applied to one month old tomato plants. After four days, plants were inoculated with Pseudomonas sp PB11. Infection severity was determined by counting the number of necrotic lesions present in each plant and it were expressed as damage percentage. The extract led to a significantly infection reduction of more than 60% (p<0.05 . The protection effect was observed on the fifth day after inoculation and it was maintained for the next 30 days. This study shows the potential of PB11 cell free extract to induce a defence response in tomato plants to Pseudomonas sp (PB11 under greenhouse conditions.

  8. Adequação da contagem de células somáticas e da contagem bacteriana total em leite cru refrigerado aos parâmetros da legislação

    Directory of Open Access Journals (Sweden)

    G.A. Bozo

    2013-04-01

    Full Text Available O objetivo deste trabalho foi adequar a qualidade do leite cru refrigerado de cinco propriedades leiteiras aos parâmetros microbiológicos e de contagem de células somáticas (CCS estabelecidos pela legislação estadual do Paraná. As propriedades leiteiras estudadas foram monitoradas durante sete meses após a implantação de boas práticas de ordenha e de recomendações quanto ao tratamento de mastites e à manutenção e higienização de equipamentos de ordenha. Antes da adoção das recomendações, a contagem bacteriana total (CBT média era de 1,36 x 10(6UFC/mL e a CCS média, de 1,87 x 10(6/mL. Todas as propriedades atingiram os padrões estabelecidos para CBT, com redução média de 93,4%. A redução de CCS foi, em média, 74,3%. Apenas três propriedades atingiram os valores estabelecidos para CCS. As demais atingiram valores muito próximos ao limite. A adequação aos padrões de CBT e CCS gerou aumento na renda mensal, em razão do pagamento por qualidade do produto, entre R$120,00 e R$828,00, de acordo com a produção de cada propriedade.

  9. Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant

    Science.gov (United States)

    Vasquez, Abel E; Manzo, Ricardo A; Soto, Daniel A; Barrientos, Magaly J; Maldonado, Aurora E; Mosqueira, Macarena; Avila, Anastasia; Touma, Jorge; Bruce, Elsa; Harris, Paul R; Venegas, Alejandro

    2015-01-01

    The Neisseria meningitidis outer membrane protein PorA from a Chilean strain was purified as a recombinant protein. PorA mixed with AbISCO induced bactericidal antibodies against N. meningitidis in mice. When PorA was fused to the Helicobacter pylori HpaA antigen gene, the specific response against H. pylori protein increased. Splenocytes from PorA-immunized mice were stimulated with PorA, and an increase in the secretion of IL-4 was observed compared with that of IFN-γ. Moreover, in an immunoglobulin sub-typing analysis, a substantially higher IgG1 level was found compared with IgG2a levels, suggesting a Th2-type immune response. This study revealed a peculiar behavior of the purified recombinant PorA protein per se in the absence of AbISCO as an adjuvant. Therefore, the resistance of PorA to proteolytic enzymes, such as those in the gastrointestinal tract, was analyzed, because this is an important feature for an oral protein adjuvant. Finally, we found that PorA fused to the H. pylori HpaA antigen, when expressed in Lactococcus lactis and administered orally, could enhance the antibody response against the HpaA antigen approximately 3 fold. These observations strongly suggest that PorA behaves as an effective oral adjuvant. PMID:25750999

  10. Alguns aspectos epidemiológicos da mancha bacteriana (Xanthomonas spp. do tomateiro na região de Caçador/SC Some epidemiological aspects of bacterial spot (Xanthomonas spp. of tomato in Caçador/SC, Brazil

    Directory of Open Access Journals (Sweden)

    Leandro Luiz Marcuzzo

    2009-06-01

    Full Text Available Objetivando explorar alguns aspectos da epidemiologia da de orvalho e um período médio de 13 horas continuas de umidade mancha bacteriana do tomateiro, incitada por Xanthomonas spp., relativa e"90%. A população bacteriana epifítica oscilou nas 10 em Caçador/SC, um ensaio a campo foi conduzido com plantas semanas após o plantio, conforme as condições climáticas, no inoculadas antes do transplantio. A cada sete dias e durante 19 entanto após o inicio dos sintomas manteve-se estável. O semanas foi monitorada a população bacteriana epifítica, as progresso da doença foi representado pelo modelo logístico y = condições climáticas e a severidade na planta. Constatou-se que o 0.99964/(1+exp(10.35989-0.69762*x e devido a pratica de inicio da epidemia teve concomitância com início da maturação apenas 1 colheita semanal, a severidade em frutos foi alta, fisiológica dos frutos do primeiro cacho, sendo que 77 dias antes atingindo 30,22% com produtividade total de117,88 ton.ha-1. Este do início da colheita não houve sintomas nas folhas. Observou-se, estudo epidemiológico servirá de um indicativo para determinação que mesmo em condição de estiagem, houve acréscimo da doença do inicio da epidemia e será usado na validação de um sistema de devido ao constante molhamento foliar decorrente da formação previsão para a mancha bacteriana do tomateiro.Aiming to explore some epidemiological aspects of bacterial spot of tomato incited by Xanthomonas spp., a field experiment was carried out in Caçador, SC. Tomato plants were inoculated with a bacterial suspension before transplanting. At seven-day intervals the bacterial population on leaf surface and disease severity were monitored during 19 weeks. Daily weather records were registered during the crop season. It was observed that epidemic onset coincided with physiological maturation of the first fruit clusters. Leaves were symptomless until 77 days before harvest. It was

  11. Bacterial Levan: tecnological aspects, characteristics and production/ Levana Bacteriana: aspectos tecnológicos, características e produção

    Directory of Open Access Journals (Sweden)

    Crispin Humberto Garcia-Cruz

    2005-06-01

    Full Text Available Levan is an exopolysaccharide, constituted by fructose units, ? (2- 6 linked, obtained by transfructosilation reaction during microorganisms fermentation in a sucrose rich but wi thout glucose, fructose or mixtures in the culture media. Bacterial levan production is a good alternative fructose source, besides having certain functional characteristics in the human body, such as a hypocholesterolemic and an anticarcinogenic agent. In the food industry, levan can be used to fix colors and flavors, as well as thickening and stabilizing agent. In the bacterial levan production, Zymomonas mobilis has been considered the best possible alternative, since it uses as carbon source sucrose or industrial residues that contain this sugar, in different concentrations, in a mineral salts rich medium. Levan production is not only influenced by carbon source and its concentration, but also by pH, temperature and type of salts. Moreover, the oxygenation of the fermentation medium, also affect the characteristics of the molecule and the cellular growth. In this revision some important topics concerning the bacterial levan production are presented.Levana é um exopolissacarídeo, constituído por unidades de frutose, unidas através de ligações ? (2- 6, obtido pela reação de transfrutosilação durante a fermentação de microrganismos em meio rico em sacarose mas não em frutose, glucose ou misturas de ambas. Pesquisas sobre bactérias produtoras de levana vêm sendo implementadas, uma vez que a mesma é uma fonte alternativa de frutose, além de apresentar características funcionais no organismo humano, como agente hipocolesterolêmico e anticarcinogênico. Na indústria de alimentos a levana pode ser empregada como fixador de cores e sabores, bem como espessante e estabilizante de vários alimentos. Das bactérias produtoras de levana, Zymomonas mobilis tem sido a melhor alternativa, uma vez que usa como fonte de carbono a sacarose ou resíduos industriais

  12. Evaluación de la transferencia de oxígeno en cultivos con lactococcus lactis empleando un sistema de fermentación con aireación externa

    Directory of Open Access Journals (Sweden)

    Andrea Soler

    2010-10-01

    Full Text Available Título en inglés: Evaluating oxygen transfer in a Lactococcus lactis cultures using an external aeration fermentation system (EAFS Resumen En fermentaciones aerobias el oxígeno, como aceptor terminal de electrones en el proceso de respiración, comúnmente se constituye en limitante debido entre otros factores al diseño del biorreactor (factores geométricos, a las condiciones de operación de los fermentadores (condiciones ambientales requeridas en el cultivo, potencia transferida al cultivo por el sistema de agitación, propiedades del medio líquido, demanda de oxígeno por parte del microorganismo, sistema de aireación (concentración de oxígeno en el gas, solubilidad del oxígeno. La limitación de oxígeno se refleja en la fermentación con Lactococcus lactis cepa IBUN 34.1, en que presenta una baja disponibilidad de oxígeno desde muy temprano en la fase exponencial del cultivo. Para superar estas limitaciones se diseñó y desarrolló un sistema de suministro de oxígeno de alta tasa de transferencia, consistente en un sistema de fermentación con aireación externa (SFAE, el cual es comparado en este trabajo con el sistema tradicional de fermentador agitado dotado con dos turbinas tipo Rushton y aireación por difusor interno. En este trabajo se evalúa la operación del SFAE, se seleccionan y estudian algunas variables operacionales y su efecto sobre la transferencia de oxígeno gas-líquido. Los resultados indican que las variables que tienen efecto significativo sobre el coeficiente volumétrico global de transferencia de masa kLa son la agitación y el flujo de medio de cultivo que circula por el aireador externo denominado flujo de recirculación. Los valores de kLa obtenidos indican que con el fermentador convencional con aireación interna el mayor valor de kLa alcanzado fue de 40,68 (h-1, en tanto que con el SFAE se alcanzaron valores de 63,18 (h-1. Palabras clave: biorreactores; kLa; transferencia de ox

  13. Escova dental e dedeira na remoção da placa bacteriana dental em cães The dental brush and thumb-stall in the removal of the dental plaque in dogs

    OpenAIRE

    Tânia Berbert Ferreira Lima; Duvaldo Eurides; Renata Junqueira Rezende; Vanessa Martins Fayad Milken; Luiz Antonio Franco da Silva; Maria Clorinda Soares Fioravanti

    2004-01-01

    A placa bacteriana é fator primário na formação de gengivite, cálculo dentário, halitose e doença periodontal. Objetivou-se avaliar a quantidade de placa bacteriana dental removida pela escova dental e dedeira. Foram utilizados 60 cães machos e fêmeas de diferentes raças, idade e peso, divididos em dois grupos. O índice Logan & Boyce foi utilizados para quantificar a placa bacteriana antes e após a escovação. Observou-se diferença estatística (p0,05) entre a utilização da escova dental e a de...

  14. Plasmid elimination of Lactococcus lactis%乳酸乳球菌的质粒消除

    Institute of Scientific and Technical Information of China (English)

    柳国霞; 范丽平; 霍贵成

    2009-01-01

    质粒消除是签定质粒和获得无质粒菌株的重要方法,是乳酸菌进行遗传学改造所需的一项重要技术.试验采用高温和消除剂结合的方法,对乳酸乳球菌镉抗性菌株进行质粒消除,探讨温度、消除剂吖啶橙的用量和作用时间对乳酸乳球菌镉抗性质粒消除的影响.结果表明,39℃高温可以质粒消除,而37和41℃均无此效果;独自吖叮橙作用未获得质粒消除菌株;39℃高温-吖啶橙同时作用比高温-吖啶橙交替作用消除率高,而39℃高温-20 μg·mL-1吖啶橙共同作用12 d,消除率可达98%.根据消除结果,以疑似功能性质粒为模板,进行PCR扩增,获得预期片段,进一步证实了其功能.%Plasmid elimination is a key method of plasmid identificaton and obtaining non-plasmid strain, and it is an important technology which was needed by genetics transformation in lactic acid bacteria. High temperature and the elimination agent were combined by plasmid elimination for a cadmium-resistant Lactococcus lactis strains, the amount and the time by using temperature and eUminaton agent acridine orange (AO) were approached on the effect of cadmium-resistant Lactococcus lactis plasmid. The results showed that 39℃ high temperature could eliminate the plasmid, but 37 and 41℃ had no effects. Plasmid elimination strain was not obtained by using AO alone, the elimination rate by the method of both high temperature and AO at the same time was higher than that of by interchanging between high temperature and AO. The combined effect of 39℃ temperature and 20 μg·mL-1 AO for 12 days was significant, the elimination rate was as high as 98%. According to the results, PCR amplification was based on the suspected functional plasmid as a template, achieved the desired fragment, and further confirmed its functions.

  15. Control of tomato bacterial wilt through the incorporation of aerial part of pigeon pea and crotalaria to soil Controle da murcha bacteriana do tomateiro pela incorporação da parte aérea de guandu e crotalária no solo

    Directory of Open Access Journals (Sweden)

    Suane C. Cardoso

    2006-03-01

    aérea de guandu e crotalária foi incubado por 30 e 60 dias, antes do plantio. Mudas de tomateiro 'Santa Clara' foram transplantadas para sacos de polietileno contendo 3 kg de substrato (solo infestado + matéria orgânica e avaliou-se, por um período de 45 dias, o sintoma de murcha bacteriana e a percentagem de plantas com floração. A incorporação e incubação por 30 dias com guandu e crotalária promoveu 100 % de controle da murcha, em todas as concentrações avaliadas. Com 60 dias de incubação, apenas a concentração de 10 % de guandu e crotalária não controlou a doença. Esses resultados indicam que a incorporação no solo com a parte aérea de guandu e crotalária é um método eficiente para o controle da murcha bacteriana do tomateiro.

  16. Contaminación bacteriana en concentrados de plaquetas de caballos Bacterial contamination in platelet concentrates of horses

    Directory of Open Access Journals (Sweden)

    ME Álvarez

    2010-01-01

    Full Text Available Los objetivos del estudio fueron: 1 evaluar el riesgo de contaminación bacteriana de concentrados autólogos de plaquetas (APCs de caballos obtenidos mediante el método del tubo en tres condiciones técnicas diferentes (cámara de flujo laminar y en ambiente de laboratorio limpio con mechero o sin mechero, 2 identificar los puntos críticos del proceso de preparación de los APCs con posibilidad de contaminación bacteriana y 3 identificar las bacterias potencialmente contaminantes en el proceso. Se tomaron muestras bacteriológicas de la piel (rasurada o no del sitio de venopunción de 15 caballos, antes y después de ser desinfectados; manos y garganta del operario; tapones de los tubos donde se procesó la sangre; medio ambiente donde se tomaron las muestras de sangre, ambiente de laboratorio, cámara de flujo laminar, estufa bacteriológica y de los APCs obtenidos bajo condiciones técnicas. Se aislaron bacterias de la piel equina sin desinfectar, manos y garganta del operario y del lugar donde se tomaron las muestras de sangre. No se aislaron bacterias de los tapones de los tubos, medio ambiente del laboratorio, cámara de flujo laminar, ni de los APCs. Las bacterias aisladas fueron biota normal de la piel equina, de la piel y garganta humana, y contaminantes medioambientales. En conclusión, los APCs pueden ser obtenidos mediante el método del tubo en un ambiente limpio.The aims of the study were to: 1 assess the risk of bacterial contamination in equine platelet concentrates (PCs obtained by the tube method under three technical conditions (laminar flow cabinet or in a clean laboratory environment both with burner and without burner 2 identify the critical points of the process of PCs preparation with risk of bacterial contamination; and 3 identify the potential bacterial contaminants in the process. Bacteriological samples were taken from the skin (shaved or unshaved of the venipuncture site in 15 horses, both before and after being

  17. Vaginose Bacteriana em Mulheres com Infertilidade e em Menopausadas Bacterial Vaginosis In Menopausal Women and in Women with Infertility

    Directory of Open Access Journals (Sweden)

    Miriam da Silva Wanderley

    2001-12-01

    Full Text Available Objetivo: analisar a prevalência de vaginose bacteriana (VB em mulheres inférteis e em menopausadas e os métodos mais comumente usados na prática clínica para o seu diagnóstico. Métodos: foram avaliadas retrospectivamente 104 pacientes na menopausa e 86 inférteis. A presença de corrimento vaginal característico, pH vaginal >4,5, teste das aminas (whiff test positivo e achado de vaginose bacteriana à coloração da secreção pelo Gram foram considerados positivos. Foi estabelecido diagnóstico de VB quando 3 dos 4 critérios acima fossem satisfeitos. Resultados: analisando os métodos diagnósticos separadamente observamos, entre as menopausadas, 29 pacientes com corrimento vaginal característico (28,1%, 10 (9,6% com whiff test positivo, 68 (65,4% com pH vaginal >4,5 e 34 (32,7% com teste do Gram positivo. Nas mulheres inférteis os resultados foram 20 (23,2%, 13 (15,1%, 61 (70,9% e 26 (30,2%, respectivamente. Ao analisarmos todos os critérios em conjunto, em 14 pacientes na menopausa (13,5% e em 15 inférteis (17,4% foi diagnosticada VB. Conclusão: a prevalência de VB foi similar nos 2 grupos de pacientes. Além disso, todos os métodos diagnósticos devem ser utilizados a fim de não se sub ou super-diagnosticar essa patologia.Purpose: to evaluate the prevalence of bacterial vaginosis (BV in menopausal and in infertile outpatients and to analyze the current clinical diagnostic methods. Methods: we evaluated retrospectively 104 menopausal women and 86 with infertility. Characteristic vaginal discharge on gynecological examination, pH >4.5, positive KOH whiff test, and bacterial vaginosis by Gram test were considered positive. BV was established when at least 3 out of 4 criteria were found. Results: among the menopausal women, 29 patients (28.1% were clinically positive for BV, 10 (9.6% had positive whiff test, 68 (65.4% vaginal pH >4.5, and 34 (32.7% positive Gram test. For the infertile patients the figures were 20 (23.2%, 13

  18. Detection and characterization of bacteriocin-producing Lactococcus lactis strains Detecção e caracterização de Lactococcus lactis produtores de bacteriocinas

    Directory of Open Access Journals (Sweden)

    Izildinha Moreno

    1999-04-01

    Full Text Available One hundred sixty seven strains of Lactococcus lactis were screened for bacteriocin production by well diffusion assay of GM17 agar. Fourteen (8.4% produced antimicrobial activity other than organic acids, bacteriophages or hydrogen peroxide. The frequency of bacteriocin production ranged from 2% in L. lactis subsp. cremoris up to 12% in L. lactis subsp. lactis. Antimicrobial activities were not observed in any strain of L. lactis subsp. lactis var. diacetylactis. Among thirteen bacteriocin-producing strains and two nisin-producing strains (L. lactis subsp. lactis ATCC 11454 and L. lactis subsp. lactis CNRZ 150, eight (53% were characterized as lactose-positive (Lac+ and proteinase-negative (Prt-. The bacteriocin-producing cultures were also characterized on the basis of plasmid content. All strains had 2 to 7 plasmids with molecular weights varying from 0.5 to 28.1 Mdal. Four strains (ITAL 435, ITAL 436, ITAL 437 and ITAL 438 showed identical profiles and the other were quite distinct.Um total de 167 linhagens de L. lactis foi selecionado para os testes de produção de bacteriocinas pelo método de difusão em poços em agar GM17. Desse total, 14 (8.4% produziram substâncias inibidoras que não foram associadas com ácidos orgânicos, peróxido de hidrogênio e bacteriófagos. A frequência de produção de bacteriocinas variou de 2% em L. lactis subsp. cremoris a 12% em L. lactis subsp. lactis. Nenhuma das linhagens de L. lactis subsp. lactis var. diacetylactis produziu substâncias inibidoras. De 13 linhagens produtoras de bacteriocinas e duas de nisina (L. lactis subsp. lactis ATCC 11454 e L. lactis subsp. lactis CNRZ 150, 8 (53% foram caracterizadas como lactose-positivas (Lac+ e proteinase-negativas (Prt-. As linhagens produtoras de bacteriocinas também foram caracterizadas no seu conteúdo de plasmídios. Elas apresentaram de 2 a 7 plasmídios, com pesos moleculares aproximados de 0.5 a 28.1 Mdal. Quatro linhagens (ITAL 435, ITAL 436

  19. Tireoidite bacteriana supurativa: relato de caso e revisão da literatura Acute suppurative thyroiditis: a case report and review of the literature

    Directory of Open Access Journals (Sweden)

    Adriana Vieira Pedreira

    2002-11-01

    Full Text Available Neste trabalho é apresentado um caso de tireoidite bacteriana aguda em um criança lúpica de nove anos de idade, em que o diagnóstico precoce foi imprescindível pela gravidade do quadro. Em seguida é apresentada uma revisão da literatura sobre o assunto.We present the case of a 9-year-old girl with acute suppurative thyroiditis and systemic lupus erythematosus. Early diagnosis was crucial for the outcome of the patient due to the severity of the disease. A review of the literature is also presented.

  20. Biofilme de blenda de fécula de batata e celulose bacteriana na conservação de fruta minimamente processada

    OpenAIRE

    Almeida, Denise Milléo

    2011-01-01

    Resumo: A eficiência dos biofilmes comestíveis está ligada às propriedades dos biopolímeros utilizados como base. Os polissacarídeos de origem microbiana apresentam-se como nova perspectiva, na influencia das propriedades de barreira e mecânica dos biofilmes e, auxilio no método de conservação. O objetivo deste trabalho foi avaliar o efeito de biofilme formado de blenda de fécula de batata e celulose bacteriana na conservação de fruta minimamente processada. O trabalho foi desenvolvido em trê...

  1. Estratégias para prevenção da resistência bacteriana: contribuições para a segurança do paciente

    Directory of Open Access Journals (Sweden)

    Roberta Soldatelli Pagno Paim

    2014-11-01

    Full Text Available Introdução: A resistência bacteriana tem como consequências, o aumento da morbidade, mortalidade e da internação hospitalar, o prolongamento das doenças, a perda da produtividade, o aumento dos custos e maior risco de complicações. Materiais e Métodos: Trata-se de um estudo descritivo, com abordagem qualitativa, na perspectiva de uma revisão integrativa da literatura, realizada em janeiro de 2014. Resultados e Discussão: Foram selecionados oito artigos que relatavam estratégias de prevenção da resistência bacteriana em um recorte temporal de 2008 a 2014. O uso racional de antimicrobianos, a higienização adequada das mãos, a cultura de vigilância microbiológica, a educação continuada, a desinfecção de superfícies, uso de testes de suscetibilidade, o isolamento de contato, quando indicado e a manutenção de um banco de dados, são alguns dos métodos encontrados neste trabalho para prevenir a seleção de micro-organismos resistentes. Conclusões: A diminuição do lançamento de novos antimicrobianos pela indústria farmacêutica e a necessidade de novos antibióticos reforça ainda mais a importância da conscientização de toda a equipe multidisciplinar no processo de prevenção de resistência antimicrobiana.            Palavras chave: Farmacorresistência Bacteriana, Antibacterianos, Programa de Controle de Infecção Hospitalar, Infecção Hospitalar, Segurança do Paciente. (Fonte: DeCS BIREME.Cómo citar este artículo: Paim R, Lorenzini E. Estrategias para prevenção da resistência bacteriana. Rev Cuid. 2014; 5(2:757-64. http://dx.doi.org/10.15649/cuidarte.v5i2.88

  2. Estratégias para prevenção da resistência bacteriana: contribuições para a segurança do paciente

    OpenAIRE

    Roberta Soldatelli Pagno Paim; Elisiane Lorenzini

    2014-01-01

    Introdução: A resistência bacteriana tem como consequências, o aumento da morbidade, mortalidade e da internação hospitalar, o prolongamento das doenças, a perda da produtividade, o aumento dos custos e maior risco de complicações. Materiais e Métodos: Trata-se de um estudo descritivo, com abordagem qualitativa, na perspectiva de uma revisão integrativa da literatura, realizada em janeiro de 2014. Resultados e Discussão: Foram selecionados oito artigos que relatavam estratégias de prevenção d...

  3. Caracterización de pacientes con bronconeumonía bacteriana asociada a la ventilación artificial mecánica

    OpenAIRE

    Danilo Eranio Utra Cardoso; Leonila Atencio González; Aida Esther Leyva Peña; Gladis Marrero Ávila; Idalia Arcias Castillo

    2015-01-01

    Fundamento: las neumonías nosocomiales constituyen hoy en día un problema de salud a nivel mundial. En las unidades de cuidados intensivos de las instituciones hospitalarias ingresa un elevado número de pacientes que requieren ventilación artificial mecánica, factor de riesgo asociado a este tipo de infecciones. Objetivo: caracterizar a los pacientes con neumonías bacterianas asociadas a la ventilación artificial mecánica, ingresados en la sala de cuidados intensivos del Hospital General D...

  4. Genética y genómica enfocadas en el estudio de la resistencia bacteriana Genetics and Genomics for the study of bacterial resistance

    OpenAIRE

    Ulises Garza-Ramos; Jesús Silva-Sánchez; Esperanza Martínez-Romero

    2009-01-01

    La resistencia bacteriana es un problema de salud pública causante de índices elevados de morbi-mortalidad hospitalaria. En la medida en que se usan los diferentes antibióticos se seleccionan bacterias resistentes a múltiples fármacos. El desarrollo de nuevas herramientas moleculares de la genómica y proteómica, como el PCR en tiempo real, pirosecuenciación de ADN, espectrometría de masas, microarreglos de ADN y bioinformática, permite conocer en forma más estrecha la fisiología y estructura ...

  5. Análisis de costo/efectividad en Colombia de los tratamientos antimicrobianos para la vaginosis bacteriana en mujeres sintomáticas no gestantes

    OpenAIRE

    Díaz Ortega, Miguel Hernando

    2012-01-01

    Introducción: existen diferentes recomendaciones para el tratamiento de la vaginosis bacteriana, sin embargo, no existe evidencia en nuestro país de la razón de costo/efectividad de estas tecnologías. Objetivo:estimar desde la perspectiva del tercer pagador la razón de costo/efectividad promedio e incremental en Colombia de la monoterapia con metronidazol oral en multidosis, metronidazol intravaginal en gel, clindamicina oral en multidosis y clindamicina intravaginal en crema, comparados c...

  6. Estudi de la població bacteriana endobronquial en la Malaltia Pulmonar Obstructiva Crònica. Aplicació del raspall protegit

    OpenAIRE

    Rosell i Gratacòs, Antoni

    2001-01-01

    Descripció del recurs: 6 març 2002 Títol obtingut de la portada digitalitzada La caracterització de la població bacteriana de les vies respiratòries baixes dels pacients amb Malaltia Pulmonar Obstructiva Crònica (MPOC) a partir de l'esput, tant en fase estable com en fase d'agudització, ha d'interpretar-se amb cautela per la inherent contaminació orofaringea de la mostra. Per aquest motiu, s'han intentat buscar mètodes alternatius com l'aspirat transtraqueal o les tècniques fibrobroncos...

  7. Diversidad bacteriana en un biorreactor de lecho fluidificado durante el tratamiento de agua contaminada con nafta Bacterial diversity in a fluidized bed bioreactor (FBR) treating gasoline-contaminated groundwater

    OpenAIRE

    S. L. Ara-Rojas; Massol-Deyá, A

    2007-01-01

    El objetivo principal de esta investigación fue determinar la diversidad bacteriana del proceso de biorremediación de agua contaminada con nafta en un biorreactor de lecho fluidificado en el Recinto Universitario de Mayagüez, de la Universidad de Puerto Rico. El aislamiento y la caracterización de las colonias bacterianas del sistema de biorremediación fueron realizados en medio R2A. Las pruebas morfológicas incluyeron la determinación de la morfología celular y de las colonias, y la reacción...

  8. Tratamento da vaginose bacteriana com gel vaginal de Aroeira (Schinus terebinthifolius Raddi): ensaio clínico randomizado Treatment of bacterial vaginosis with Schinus terebinthifolius Raddi vaginal gel: a randomized controlled trial

    OpenAIRE

    Melania Maria Ramos Amorim; Luiz Carlos Santos

    2003-01-01

    OBJETIVOS: testar a eficácia e a tolerância do gel de aroeira (Schinus terebinthifolius Raddi) para tratamento da vaginose bacteriana. MÉTODOS: quarenta e oito mulheres com vaginose bacteriana sintomática (de acordo com os critérios de Amsel) foram incluídas em ensaio clínico randomizado, duplo-cego, controlado, comparando-se o uso do gel vaginal de aroeira (25 casos) com placebo (23 casos). Os principais desfechos avaliados foram: taxa de cura, presença de lactobacilos na colpocitologia depo...

  9. Composição diferencial das comunidades bacterianas na rizosfera de variedas de cana-de-açúcar

    Directory of Open Access Journals (Sweden)

    Diogo Paes da Costa

    2014-12-01

    Full Text Available A cultura da cana-de-açúcar é de extrema importância no cenário agrícola nacional. No entanto, pouco se sabe sobre a estruturação das comunidades microbianas associadas aos solos e às rizosferas de tais plantas. O objetivo deste trabalho foi avaliar a estrutura e diversidade das comunidades de bactérias associadas ao solo e à rizosfera de seis variedades de cana-de-açúcar cultivadas no Estado de São Paulo (Brasil. As análises foram realizadas com base em métodos independentes de cultivo, em que a técnica de PCR-DGGE revelou alterações na rizosfera para os grupos de bactérias totais e também para os grupos de Alphaproteobacteria e Betaproteobacteria. Após essa análise, quatro amostras (três de rizosfera e uma de solo foram usadas para o sequenciamento da região V6 do gene 16S DNAr na plataforma Ion Torrent TM. Essa análise gerou um total de 95.812 sequências, dentro das quais houve a predominância das afiliadas aos filos Actinobacteria, Proteobacteria e Acidobateria . Os resultados revelaram que as comunidades bacterianas na rizosfera são distintas daquelas encontradas no solo. Foi possível ainda observar efeito diferencial de plantas das variedades. Alguns grupos bacterianos apresentaram menor frequência na rizosfera (Acidobacteria , enquanto outros se mostraram fortemente estimulados pela presença das raízes, comumente para todas as variedades (Betaproteobacteria , Nitrospora e Chloroflexi , ou em respostas variedade-específicas (Bacilli e Sphingobacteria .

  10. IDENTIFICACIÓN MOLECULAR DE POBLACIONES BACTERIANAS ASOCIADAS AL CARACOL PALA (Strombus gigas DEL CARIBE COLOMBIANO Molecular Identification Of Bacterial Populations Associated To Queen Conch (Strombus gigas From Colombian Caribbe

    Directory of Open Access Journals (Sweden)

    EDINSON ANDRÉS ACOSTA

    Full Text Available El caracol Pala, Strombus gigas (Strombidae, es de gran importancia ecológica y socioeconómica en el área caribeña colombiana. Sin embargo, es una especie catalogada como "vulnerable" y existe muy poca información referente a las especies bacterianas asociadas al caracol que puedan ser importantes para el desarrollo, manejo productivo y de seguridad acuícola de estos gastrópodos. En este trabajo, nosotros empleamos un estudio microbiológico y molecular de la región intergénica entre los genes 16S y 23S rDNA, análisis del gen rDNA 16S y secuenciación, para analizar las bacterias asociadas al caracol Pala (S. gigas. La composición de bacterias cultivables asociadas fue evaluada por su capacidad para crecer en agar marino y en medios de cultivos selectivos. De un total de 28 muestras analizadas encontramos que el número de bacterias cultivadas en condiciones aerobias fue de alrededor 10(6 ufc mL-1 donde las bacterias pertenecientes a la familia Vibrionacea fueron las más abundantes, cerca de >10(5 ufc mL-1. El análisis molecular de la región intergénica entre los genes 16S y 23S rDNA de las diferentes muestras, reveló una gran complejidad bacteriana asociada a S. gigas. Las secuencias de los amplificados del gen rDNA 16S identificó Pseudoalteromonas sp., Halomonas sp., Psycrobacter sp., Cobetia sp., Pseudomonas sp. y Vibrios sp. Nuestros resultados podrían sugerir un rol importante de estas bacterias como componentes de la comunidad asociada al S. gigas. Esta información puede complementar los estudios que se están implementando en los procesos para la conservación y repoblamiento de las poblaciones de S. gigas en Colombia.The Queen Conch, Strombus gigas (Strombidae, is a species of great ecological and socioeconomic importance in the Caribbean area of Colombia. However, it is currently catalogued as "vulnerable"; there is limited information concerning the bacterial species associated with conch and important in the

  11. Variable volume fed-batch fermentation for nisin production by Lactococcus lactis subsp. lactis W28.

    Science.gov (United States)

    Wu, Zhaoliang; Wang, Lin; Jing, Yingjun; Li, Xueliang; Zhao, Yanli

    2009-03-01

    A feeding technology that was suitable for improving the nisin production by Lactococcus lactis subsp. lactis W28 was established. The effects of initial sucrose concentration (ISC) in the fermentation broth, feeding time, and feeding rate on the fermentation were studied. It was observed that a fed-batch culture (ISC = 10 g l(-1)) with 100 ml sucrose solution (190 g l(-1)) being evenly fed (9-10 ml h(-1)) into the fermenter after 3-h fermentation gave the best performance in terms of biomass and nisin yield. Under these conditions, the total biomass and the total nisin yield were approximately 23% and 51% higher than those in batch fermentation, respectively. When the sucrose concentration was controlled at 5-10 g l(-1) in variable volume intermittent fed-batch fermentation (VVIF) with ISC = 10 g l(-1), the total biomass and the total nisin yield were 29% and 60% above those in batch fermentation, respectively. The VVIF proved to be effective to eliminate the substrate inhibition by maintaining sucrose at appropriate levels. It is also easy to be scaled up, since various parameters involved in industrial production were taken into account. PMID:18712289

  12. Exploring optimization parameters to increase ssDNA recombineering in Lactococcus lactis and Lactobacillus reuteri.

    Science.gov (United States)

    Van Pijkeren, Jan-Peter; Neoh, Kar Mun; Sirias, Denise; Findley, Anthony S; Britton, Robert A

    2012-01-01

    Single-stranded DNA (ssDNA) recombineering is a technology which is used to make subtle changes in the chromosome of several bacterial genera. Cells which express a single-stranded DNA binding protein (RecT or Bet) are transformed with an oligonucleotide which is incorporated via an annealing and replication-dependent mechanism. By in silico analysis we identified ssDNA binding protein homologs in the genus Lactobacillus and Lactococcus lactis. To assess whether we could further improve the recombineering efficiency in Lactobacillus reuteri ATCC PTA 6475 we expressed several RecT homologs in this strain. RecT derived from Enterococcus faecalis CRMEN 19 yielded comparable efficiencies compared with a native RecT protein, but none of the other proteins further increased the recombineering efficiency. We successfully improved recombineering efficiency 10-fold in L. lactis by increasing oligonucleotide concentration combined with the use of oligonucleotides containing phosphorothioate-linkages (PTOs). Surprisingly, neither increased oligonucleotide concentration nor PTO linkages enhanced recombineering in L. reuteri 6475. To emphasize the utility of this technology in improving probiotic features we modified six bases in a transcriptional regulatory element region of the pdu-operon of L. reuteri 6475, yielding a 3-fold increase in the production of the antimicrobial compound reuterin. Directed genetic modification of lactic acid bacteria through ssDNA recombineering will simplify strain improvement in a way that, when mutating a single base, is genetically indistinguishable from strains obtained through directed evolution. PMID:22750793

  13. Heterologous protein secretion in Lactococcus lactis: a novel antigen delivery system

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    Langella P.

    1999-01-01

    Full Text Available Lactic acid bacteria (LAB are Gram-positive bacteria and are generally regarded as safe (GRAS organisms. Therefore, LAB could be used for heterologous protein secretion and they are good potential candidates as antigen delivery vehicles. To develop such live vaccines, a better control of protein secretion is required. We developed an efficient secretion system in the model LAB, Lactococcus lactis. Staphylococcal nuclease (Nuc was used as the reporter protein. We first observed that the quantity of secreted Nuc correlated with the copy number of the cloning vector. The nuc gene was cloned on a high-copy number cloning vector and no perturbation of the metabolism of the secreting strain was observed. Replacement of nuc native promoter by a strong lactococcal one led to a significant increase of nuc expression. Secretion efficiency (SE of Nuc in L. lactis was low, i.e., only 60% of the synthesized Nuc was secreted. Insertion of a synthetic propeptide between the signal peptide and the mature moiety of Nuc increased the SE of Nuc. On the basis of these results, we developed a secretion system and we applied it to the construction of an L. lactis strain which secretes a bovine coronavirus (BCV epitope-protein fusion (BCV-Nuc. BCV-Nuc was recognized by both anti-BCV and anti-Nuc antibodies. Secretion of this antigenic fusion is the first step towards the development of a novel antigen delivery system based on LAB-secreting strains.

  14. Induced Levels of Heat Shock Proteins in dnaK mutants of Lactococcus lactis

    DEFF Research Database (Denmark)

    Koch, Birgit; Hammer, Karin; Vogensen, Finn K.;

    1998-01-01

    The bacterial heat shock response is characterized by the elevated expression of a number of chaperone complexes and proteases including the DnaK-GrpE-DnaJ and the GroELS chaperone complexes. In order to investigate the importance of the DnaK chaperone complex for the growth and the heat shock...... the inferred substrate binding site of the DnaK protein, exhibits a pronounced temperature sensitive phenotype and shows altered regulation of the heat shock response. The expression of the heat shock proteins are increased at the normal growth temperature measured both as protein synthesis rates and...... mRNA levels which indicate that DnaK could be involved in the regulation of the heat shock response in Lactococcus lactis. In Bacillus subtilis it has been found by Mogk,A., G.Homouth, C. Scholz, L. Kim, F.X. Schmid, and W. Schumann. 1997. EMBO J. 16: 4579-4590, that the activity of the heat shock...

  15. Adaptation of Lactococcus lactis to high growth temperature leads to a dramatic increase in acidification rate.

    Science.gov (United States)

    Chen, Jun; Shen, Jing; Ingvar Hellgren, Lars; Ruhdal Jensen, Peter; Solem, Christian

    2015-01-01

    Lactococcus lactis is essential for most cheese making, and this mesophilic bacterium has its growth optimum around 30 °C. We have, through adaptive evolution, isolated a mutant TM29 that grows well up to 39 °C, and continuous growth at 40 °C is possible if pre-incubated at a slightly lower temperature. At the maximal permissive temperature for the wild-type, 38 °C, TM29 grows 33% faster and has a 12% higher specific lactate production rate than its parent MG1363, which results in fast lactate accumulation. Genome sequencing was used to reveal the mutations accumulated, most of which were shown to affect thermal tolerance. Of the mutations with more pronounced effects, two affected expression of single proteins (chaperone; riboflavin transporter), two had pleiotropic effects (RNA polymerase) which changed the gene expression profile, and one resulted in a change in the coding sequence of CDP-diglyceride synthase. A large deletion containing 10 genes was also found to affect thermal tolerance significantly. With this study we demonstrate a simple approach to obtain non-GMO derivatives of the important L. lactis that possess properties desirable by the industry, e.g. thermal robustness and increased rate of acidification. The mutations we have identified provide a genetic basis for further investigation of thermal tolerance. PMID:26388459

  16. Mechanism of flavin reduction in the class 1A dihydroorotate dehydrogenase from Lactococcus lactis

    DEFF Research Database (Denmark)

    Fagan, Rebecca L; Jensen, Kaj Frank; Björnberg, Olof; Palfey, Bruce A

    2007-01-01

    is concerted or stepwise was addressed for the class 1A enzyme from Lactococcus lactis by determining kinetic isotope effects (KIEs) on flavin reduction in anaerobic stopped-flow experiments. Isotope effects were determined at two pH values. At pH 7.0, KIEs were approximately 2-fold for DHO labeled....... These isotope effects are consistent with a concerted oxidation of DHO. The pH dependence of reduction was also determined, and a pKa of 8.3 was found. This pKa can be attributed to the ionization of the active site cysteine which deprotonates C5 of DHO during the reaction. To further investigate the...... importance of the active site base, two site-directed mutants were also studied: Cys130Ala (removal of the active site base) and Cys130Ser (replacement with the active site base used by class 2 DHODs). Both mutant enzymes exhibited binding affinities for DHO similar to that of the wild-type enzyme. Reduction...

  17. A genome-scale integration and analysis of Lactococcus lactis translation data.

    Directory of Open Access Journals (Sweden)

    Julien Racle

    Full Text Available Protein synthesis is a template polymerization process composed by three main steps: initiation, elongation, and termination. During translation, ribosomes are engaged into polysomes whose size is used for the quantitative characterization of translatome. However, simultaneous transcription and translation in the bacterial cytosol complicates the analysis of translatome data. We established a procedure for robust estimation of the ribosomal density in hundreds of genes from Lactococcus lactis polysome size measurements. We used a mechanistic model of translation to integrate the information about the ribosomal density and for the first time we estimated the protein synthesis rate for each gene and identified the rate limiting steps. Contrary to conventional considerations, we find significant number of genes to be elongation limited. This number increases during stress conditions compared to optimal growth and proteins synthesized at maximum rate are predominantly elongation limited. Consistent with bacterial physiology, we found proteins with similar rate and control characteristics belonging to the same functional categories. Under stress conditions, we found that synthesis rate of regulatory proteins is becoming comparable to proteins favored under optimal growth. These findings suggest that the coupling of metabolic states and protein synthesis is more important than previously thought.

  18. Transcriptomic profile of aguR deletion mutant of Lactococcus lactis subsp. cremoris CECT 8666.

    Science.gov (United States)

    Del Rio, Beatriz; Linares, Daniel M; Redruello, Begoña; Martin, Maria Cruz; Fernandez, Maria; de Jong, Anne; Kuipers, Oscar P; Ladero, Victor; Alvarez, Miguel A

    2015-12-01

    Lactococcus lactis subsp. cremoris CECT 8666 (formerly GE2-14) is a dairy strain that catabolizes agmatine (a decarboxylated derivative of arginine) into the biogenic amine putrescine by the agmatine deiminase (AGDI) pathway [1]. The AGDI cluster of L. lactis is composed by five genes aguR, aguB, aguD, aguA and aguC. The last four genes are responsible for the deamination of agmatine to putrescine and are co-transcribed as a single policistronic mRNA forming the catabolic operon aguBDAC[1]. aguR encodes a transmembrane protein that functions as a one-component signal transduction system that senses the agmatine concentration of the medium and accordingly regulates the transcription of aguBDAC[2], which is also transcriptionally regulated by carbon catabolic repression (CCR) via glucose, but not by other sugars such as lactose and galactose [1], [3]. Here we report the transcriptional profiling of the aguR gene deletion mutant (L. lactis subsp. cremoris CECT 8666 ∆aguR) [2] compared to the wild type strain, both grown in M17 medium with galactose as carbon source and supplemented with agmatine. The transcriptional profiling data of AguR-regulated genes were deposited in the Gene Expression Omnibus (GEO) database under accession no. GSE59514. PMID:26697381

  19. Novel antibacterial activity of lactococcus lactis subspecies lactis z11 isolated from zabady.

    Science.gov (United States)

    Enan, Gamal; Abdel-Shafi, Seham; Ouda, Sahar; Negm, Sally

    2013-09-01

    The purpose of this study was to select and characterize a probiotic bacterium with distinctive antimicrobial activities. In this respect, Lactococcus lactis subspecies lactis Z11 (L. lactis Z11) isolated from Zabady (Arabian yoghurt) inhibited other strains of lactic acid bacteria and some food-born pathogens including Listeria monocytogenes, Bacillus cereus and staphylococcus aureus. The inhibitory activity of cell free supernatant (CFS) of L. lactis Z11 isolated from zabady was lost by proteolytic enzymes, heat resistant. Consequently, the active substance(s) of CFS was characterized as a bacteriocin. This bacteriocin has been shown to consist of protein but has no lipidic or glucidic moieties in its active molecule. Its activity was stable in the pH range 2.0 to 7.0 and was not affected by organic solvents. The L. lactis Z11 bacteriocin was produced in CFS throughout the mide to the late exponential phase of growth of the producer organism and maximum bacteriocin production was obtained at initial pH 6.5 at incubation temperature of about 30°C. PMID:24151453

  20. Phosphoglycerate Mutase Is a Highly Efficient Enzyme without Flux Control in Lactococcus lactis

    DEFF Research Database (Denmark)

    Solem, Christian; Petranovic, D.; Købmann, Brian;

    2010-01-01

    The glycolytic enzyme phosphoglycerate mutase (PGM), which catalyzes the conversion of 3-phosphoglycerate to 2-phosphoglycerate, was examined in Lactococcus lactis with respect to its function, kinetics and glycolytic flux control. A library of strains with PGM activities ranging between 15-465% of....... lactis PGM was dependent on 2,3-bisphosphoglyceric acid for activity, which showed that the enzyme is of the dPGM type in accordance with its predicted homology to dPGM enzymes from other organisms. In conclusion, PGM from L. lactis is a highly efficient catalyst, which partially explains why this enzyme...... at highly reduced PGM activities. At the wild-type level PGM operated very far from V-max. Consequently, in a strain with only 15% PGM activity, the catalytic rate of PGM was almost six times higher than in the wildtype. K-m of PGM for 3-phosphoglycerate was 1.0 m M and k(cat) was 3,200 s(-1). The L...

  1. Improvement of bovine ß-lactoglobulin production and secretion by Lactococcus lactis

    Directory of Open Access Journals (Sweden)

    S. Nouaille

    2005-03-01

    Full Text Available The stabilizing effects of staphylococcal nuclease (Nuc and of a synthetic propeptide (LEISSTCDA, hereafter called LEISS on the production of a model food allergen, bovine ß-lactoglobulin (BLG, in Lactococcus lactis were investigated. The fusion of Nuc to BLG (Nuc-BLG results in higher production and secretion of the hybrid protein. When LEISS was fused to BLG, the production of the resulting protein LEISS-BLG was only slightly improved compared to the one obtained with Nuc-BLG. However, the secretion of LEISS-BLG was dramatically enhanced (~10- and 4-fold higher than BLG and Nuc-BLG, respectively. Finally, the fusion of LEISS to Nuc-BLG resulting in the protein LEISS-Nuc-BLG led to the highest production of the hybrid protein, estimated at ~8 µg/ml (~2-fold higher than Nuc-BLG. In conclusion, the fusions described here led to the improvement of the production and secretion of BLG. These tools will be used to modulate the immune response against BLG via delivery of recombinant lactococci at the mucosal level, in a mouse model of cow's milk allergy.

  2. Induction and characterization of a lysogenic bacteriophage of Lactococcus garvieae isolated from marine fish species.

    Science.gov (United States)

    Hoai, T D; Yoshida, T

    2016-07-01

    This study investigated the presence of prophages in Lactococcus garvieae isolated from several marine fish species in Japan. Representative strains of 16 bacterial genotypes (S1-S16) selected from more than 400 L. garvieae isolates were used to induce lysogenic bacteriophages. These strains were treated with 500 ng mL(-1) freshly prepared mitomycin C. A cross-spotting assay was performed to validate the lysogenic and indicator strains. The lysogenic strains were selected for isolation and concentration of the phages. Phage DNA was digested with EcoRI for biased sinusoidal field gel electrophoresis analysis. Polymerase chain reaction (PCR) was used to detect integrated prophage DNA. Of the 16 representative bacterial genotypes, 12 strains integrated prophages as indicated by the PCR assay, and 10 phages were detected and isolated using two indicator bacterial strains. Analysis of genomic DNA showed that these phages were homologous and named as PLgT-1. Transmission electron microscopy revealed that the morphology of PLgT-1 was consistent with the virus family Siphoviridae. PCR analysis of the prophage DNA revealed that all of the S1 genotype strains were lysogenic (30/30), but none of the S16 genotype strains were lysogenic (0/30). This is the first study to investigate lysogenic bacteriophages from L. garvieae. PMID:26471724

  3. Enhance nisin yield via improving acid-tolerant capability of Lactococcus lactis F44.

    Science.gov (United States)

    Zhang, Jian; Caiyin, Qinggele; Feng, Wenjing; Zhao, Xiuli; Qiao, Bin; Zhao, Guangrong; Qiao, Jianjun

    2016-01-01

    Traditionally, nisin was produced industrially by using Lactococcus lactis in the neutral fermentation process. However, nisin showed higher activity in the acidic environment. How to balance the pH value for bacterial normal growth and nisin activity might be the key problem. In this study, 17 acid-tolerant genes and 6 lactic acid synthetic genes were introduced in L. lactis F44, respectively. Comparing to the 2810 IU/mL nisin yield of the original strain F44, the nisin titer of the engineered strains over-expressing hdeAB, ldh and murG, increased to 3850, 3979 and 4377 IU/mL, respectively. These engineered strains showed more stable intracellular pH value during the fermentation process. Improvement of lactate production could partly provide the extra energy for the expression of acid tolerance genes during growth. Co-overexpression of hdeAB, murG, and ldh(Z) in strain F44 resulted in the nisin titer of 4913 IU/mL. The engineered strain (ABGL) could grow on plates with pH 4.2, comparing to the surviving pH 4.6 of strain F44. The fed-batch fermentation showed nisin titer of the co-expression L. lactis strain could reach 5563 IU/mL with lower pH condition and longer cultivation time. This work provides a novel strategy of constructing robust strains for use in industry process. PMID:27306587

  4. Characterization and antimicrobial activity of natural isolate Lactococcus lactis subsp. lactis BGSM1-19

    Directory of Open Access Journals (Sweden)

    Strahinić Ivana

    2007-01-01

    Full Text Available The strain Lactococcus lactis subsp. lactis BGSM1-19, isolated from traditionally homemade white cheese, produces two bacteriocins: lactococcin B-like bacteriocin named bacteriocin BacSMa and bacteriocin BacSMb which have shown similarity with lacticin RM. Plasmid curing resulted in a low yield (0.33% of BacSMa- BacSMas and BacSMa- BacSMas, BacSMb-, BacSMbs derivatives. The bacteriocin biosynthesis was observed in the logarithmic phase of growth and the production plateau was reached after 8 h of incubation at 30oC, when the culture entered the early stationary phase. Biochemical characterization showed that strain BGSM1-19 retained antimicrobial activity within the pH range of 1 to 12 or after treatment at 100oC for 15 min. However, bacteriocin activity was completely lost after treatment with different proteolytic enzymes. The strain BGSM1-19 contains five plasmids. Plasmid curing indicated that genes coding for bacteriocins synthesis and immunity seem to be located on plasmids. BGSM1-19 exhibited antimicrobial activity against some pathogenic bacteria such as Salmonella paratyphi, Micrococcus flavus, Pseudomonas aeruginosa and Staphylococcus aureus.

  5. Protective effect of clove oil-supplemented fish diets on experimental Lactococcus garvieae infection in tilapia.

    Science.gov (United States)

    Rattanachaikunsopon, Pongsak; Phumkhachorn, Parichat

    2009-09-01

    The essential oils extracted from the four herbs, cinnamon (Cinnamomum verum), clove (Syzygium aromaticum), ginger (Zingiber officinale) and holy basil (Ocimum sanctum), were investigated for their antimicrobial activity and mode of action against Lactococcus garvieae, a fish pathogenic bacteria causing lactococcosis. Of all the tested oils, clove oil had the strongest inhibitory effect and exhibited a bactericidal mode of action against the pathogenic bacterium. When an intraperitoneal infection of tilapia (Oreochromis niloticus) with L. garvieae was performed, the median lethal dose (LD(50)) was determined to be 1.78x10(2) CFU/fish. For an in vivo trial, no mortality was apparent in fish fed on the fish diets supplemented with 3% (w/w) of clove oil and with 0.5% (w/w) of oxytetracycline 5 d prior to the infection with L. garvieae. These results indicate that clove oil had a protective effect on experimental L. garvieae infection in tilapia and the potential to replace antibiotics for controlling the disease. PMID:19734665

  6. Deciphering a unique biotin scavenging pathway with redundant genes in the probiotic bacterium Lactococcus lactis.

    Science.gov (United States)

    Zhang, Huimin; Wang, Qingjing; Fisher, Derek J; Cai, Mingzhu; Chakravartty, Vandana; Ye, Huiyan; Li, Ping; Solbiati, Jose O; Feng, Youjun

    2016-01-01

    Biotin protein ligase (BPL) is widespread in the three domains of the life. The paradigm BPL is the Escherichia coli BirA protein, which also functions as a repressor for the biotin biosynthesis pathway. Here we report that Lactococcus lactis possesses two different orthologues of birA (birA1_LL and birA2_LL). Unlike the scenario in E. coli, L. lactis appears to be auxotrophic for biotin in that it lacks a full biotin biosynthesis pathway. In contrast, it retains two biotin transporter-encoding genes (bioY1_LL and bioY2_LL), suggesting the use of a scavenging strategy to obtain biotin from the environment. The in vivo function of the two L. lactis birA genes was judged by their abilities to complement the conditional lethal E. coli birA mutant. Thin-layer chromatography and mass spectroscopy assays demonstrated that these two recombinant BirA proteins catalyze the biotinylation reaction of the acceptor biotin carboxyl carrier protein (BCCP), through the expected biotinoyl-AMP intermediate. Gel shift assays were used to characterize bioY1_LL and BirA1_LL. We also determined the ability to uptake (3)H-biotin by L. lactis. Taken together, our results deciphered a unique biotin scavenging pathway with redundant genes present in the probiotic bacterium L. lactis. PMID:27161258

  7. Prevention of gastrointestinal lead poisoning using recombinant Lactococcus lactis expressing human metallothionein-I fusion protein.

    Science.gov (United States)

    Xiao, Xue; Zhang, Changbin; Liu, Dajun; Bai, Weibin; Zhang, Qihao; Xiang, Qi; Huang, Yadong; Su, Zhijian

    2016-01-01

    Low-level lead poisoning is an insidious disease that affects millions of children worldwide, leading to biochemical and neurological dysfunctions. Blocking lead uptake via the gastrointestinal tract is an important prevention strategy. With this in mind, we constructed the recombinant Lactococcus lactis strain pGSMT/MG1363, which constitutively expressed the fusion protein glutathione S-transferase (GST)-small molecule ubiquitin-like modifier protein (SUMO)-metallothionein-I (GST-SUMO-MT). The thermodynamic data indicated that the average number of lead bound to a GST-SUMO-MT molecule was 3.655 and this binding reaction was a spontaneous, exothermic and entropy-increasing process. The total lead-binding capacity of pGSMT/MG1363 was 4.11 ± 0.15 mg/g dry mass. Oral administration of pGSMT/MG1363 (1 × 10(10) Colony-Forming Units) to pubertal male rats that were also treated with 5 mg/kg of lead acetate daily significantly inhibited the increase of blood lead levels, the impairment of hepatic function and the decrease of testosterone concentration in the serum, which were all impaired in rats treated by lead acetate alone. Moreover, the administration of pGSMT/MG1363 for 6 weeks did not affect the serum concentration of calcium, magnesium, potassium or sodium ions. This study provides a convenient and economical biomaterial for preventing lead poisoning via the digestive tract. PMID:27045906

  8. Evaluation of the rosemary essential oil (Rosmarinus officinalis L. as modulator of bacterial resistanceAvaliação do óleo essencial de alecrim (Rosmarinus officinalis L. como modulador da resistência bacteriana

    Directory of Open Access Journals (Sweden)

    Eudes Silva Velozo

    2012-05-01

    Full Text Available Microorganisms can be transmitted by food causing diseases in humans. The antibiotics commonly used in treatment of these diseases have shown little or no effect, and in view of the resistance that many microorganisms have acquired. This study evaluated the essential oil leaves of Rosmarinus officinalis L. as a modulator of resistance bacterial drug. The essential oil was obtained by hydrodistillation in a Clevenger apparatus for 3 hours. We tested four strains of E.coli resistant ampicillin (AMP and tetracycline (TET and four strains of Salmonella spp. Resistant to nitrofurantoin (NIT. The strains in 0.5 MacFarland scale suspension were inoculated on Mueller Hinton agar, then soaked antibiotic disks with 10 and 20?L oil pure rosemary were placed on the plates. After 24h/37 º C were measured halos around the discs. All strains tested showed susceptibility to the combined action of essential oil with antibiotics tested. The results indicate that the use of promising rosemary essential oil in combination with antibiotics to combat pathogenic bacteria. Diversos microrganismos podem ser veiculados por alimentos causando doenças nos seres humanos. Os antibióticos comumente utilizados no tratamento dessas doenças têm apresentado baixo ou nenhum efeito, tendo em vista à resistência que muitos microrganismos têm adquirido. Diante deste quadro, o objetivo desta pesquisa foi avaliar o óleo essencial das folhas de Rosmarinus officinalis L. como modulador da resistência bacteriana a drogas. O óleo essencial foi obtido através de hidrodestilação em aparelho de Clevenger por 3h. Foram testadas 4 cepas de E.coli resistentes a Amplicilina (AMP e a Tetraciclina (TET e 4 cepas de Salmonella spp. resistentes a Nitrofurantoína (NIT. As cepas em suspensão escala MacFarland 0,5 foram inoculadas em agar Mueller Hinton, em seguida os discos dos antibióticos embebidos com 10 e 20?L do óleo de alecrim puro foram dispostos sobre as placas. Após 24h/37º

  9. Aspectos clínicos e laboratoriais da mastite induzida por inoculação intramamária de Staphylococcus aureus em vacas e búfalas

    OpenAIRE

    A.M. Lazzari; M.V.S. Oliveira; B. Moreti; Guimarães, G; Krug, F.; G. Noleto; A.J. Mesquita; J.P. Neves

    2014-01-01

    Vários aspectos relacionados à mastite bubalina precisam ser esclarecidos. Muitas condutas são adaptadas a essa espécie pelo conhecimento adquirido com a espécie bovina. Com este experimento, objetivou-se evidenciar particularidades da mastite em búfalas e vacas que tiveram uma glândula inoculada com 1,0 x 103 UFC de S. aureus. Os animais foram monitorados por cultura bacteriana do leite, California Mastitis Test (CMT), e escores para avaliação da severidade da mastite. Foram utilizados os se...

  10. Nucleotide Sequence and Analysis of an orotate transporter-containing plasmid isolated from the Lactococcus lactis ssp. lactis biovar diacetylactis strain DB0410

    DEFF Research Database (Denmark)

    Defoor, Els Marie Celine; Martinussen, Jan

    A new lactococcal plasmid, pDBORO, was isolated from the Lactococcus lactis ssp. lactis biovar diacetylactis strain DB0410 responsible for the sensitivity of DB0410 towards the pyrimidine-analog 5´-fluoroorotate. The plasmid pDBORO amounts to 16404 bp and its complete nucleotide sequence has been...... molecular cloning, we identified the open reading frames on pDBORO necessary for the utilization of orotate as the sole pyrimidine source. Surprisingly, homologs are found on the Lactococcus lactis IL1403 and MG1363 chromosomes despite the fact that they are resistant towards fluoroorotate....

  11. Bacteriocinogenic Lactococcus lactis subsp: lactis DF04Mi isolated from goat milk: Evaluation of the probiotic potential

    Directory of Open Access Journals (Sweden)

    Danielle N. Furtado

    2014-09-01

    Full Text Available Lactic acid bacteria capable of producing bacteriocins and presenting probiotic potential open innovative technological applications in the dairy industry. In this study, a bacteriocinogenic strain (Lactococcus lactis subsp. lactis DF4Mi was isolated from goat milk, and studied for its probiotic potential. Lc. lactis DF4Mi was resistant to acidic pH and oxbile, presented co-aggregation with Listeria monocytogenes, and was not affected by several drugs from different generic groups, being sensitive to most tested antibiotics. These properties indicate that this Lc. lactis strain can be used for enhancement of dairy foods safety and quality, in combination with potential probiotic properties.

  12. Bacteriocinogenic Lactococcus lactis subsp. lactis DF04Mi isolated from goat milk: evaluation of the probiotic potential.

    Science.gov (United States)

    Furtado, Danielle N; Todorov, Svetoslav D; Landgraf, Mariza; Destro, Maria T; Franco, Bernadette D G M

    2014-01-01

    Lactic acid bacteria capable of producing bacteriocins and presenting probiotic potential open innovative technological applications in the dairy industry. In this study, a bacteriocinogenic strain (Lactococcus lactis subsp. lactis DF4Mi) was isolated from goat milk, and studied for its probiotic potential. Lc. lactis DF4Mi was resistant to acidic pH and oxbile, presented co-aggregation with Listeria monocytogenes, and was not affected by several drugs from different generic groups, being sensitive to most tested antibiotics. These properties indicate that this Lc. lactis strain can be used for enhancement of dairy foods safety and quality, in combination with potential probiotic properties. PMID:25477942

  13. Suitability of Lactococcus lactis subsp lactis ATCC 11454 as a protective culture for lightly preserved fish products

    DEFF Research Database (Denmark)

    Wessels, Stephen Wallace; Huss, Hans Henrik

    1996-01-01

    This study is part of strategy to control the human pathogen Listeria monocytogenes in lightly preserved fish products by using food-grade lactic acid bacteria. When the nisin-producing Lactococcus lactis subsp lactis ATCC 11454 was cultured in the same vessel as L-monocytogenes Scott A in brain......-heart infusion broth (BHI) at 30-degrees C, the pathogen declined from 5x10(5) to fewer than 5 cfu ml(-1) within 31 h. The effect was not due to lactic acid inhibition. Growth and nisin production by L- lactis ATCC 11454 were investigated under the conditions of temperature and salt used for light preservation...

  14. Draft Genome Sequence of Lactococcus lactis subsp. lactis bv. diacetylactis CRL264, a Citrate-Fermenting Strain.

    Science.gov (United States)

    Zuljan, Federico; Espariz, Martín; Blancato, Victor S; Esteban, Luis; Alarcón, Sergio; Magni, Christian

    2016-01-01

    We report the draft genome sequence of Lactococcus lactis subsp. lactis bv. diacetylactis CRL264, a natural strain isolated from artisanal cheese from northwest Argentina. L. lactis subsp. lactis bv. diacetylactis is one of the most important microorganisms used as starter culture around the world. The CRL264 strain constitutes a model microorganism in the studies on the generation of aroma compounds (diacetyl, acetoin, and 2,3-butanediol) by lactic acid bacteria. Our genome analysis shows similar genetic organization to other available genomes of L. lactis bv. diacetylactis strains. PMID:26847906

  15. Expression of PprI from Deinococcus radiodurans Improves Lactic Acid Production and Stress Tolerance in Lactococcus lactis

    OpenAIRE

    Dong, Xiangrong; Tian, Bing; Dai, Shang; Li, Tao; Guo, Linna; Tan, Zhongfang; JIAO, Zhen; Jin, Qingsheng; Wang, Yanping; Hua, Yuejin

    2015-01-01

    PprI is a general switch protein that regulates the expression of certain proteins involved in pathways of cellular resistance in the extremophilic bacterium Deinococcus radiodurans. In this study, we transformed pprI into Lactococcus lactis strain MG1363 using the lactococcal shuttle vector pMG36e and investigated its effects on the tolerance and lactic acid production of L. lactis while under stress. PprI was stably expressed in L. lactis as confirmed by western blot assays. L. lactis expre...

  16. In Situ Determination of the Intracellular pH of Lactococcus lactis and Lactobacillus plantarum during Pressure Treatment

    OpenAIRE

    Molina-Gutierrez, Adriana; Stippl, Volker; Delgado, Antonio; Gänzle, Michael G.; Rudi F. Vogel

    2002-01-01

    Hydrostatic pressure may affect the intracellular pH of microorganisms by (i) enhancing the dissociation of weak organic acids and (ii) increasing the permeability of the cytoplasmic membrane and inactivation of enzymes required for pH homeostasis. The internal pHs of Lactococcus lactis and Lactobacillus plantarum during and after pressure treatment at 200 and 300 MPa and at pH values ranging from 4.0 to 6.5 were determined. Pressure treatment at 200 MPa for up to 20 min did not reduce the vi...

  17. Identification of the Minimal Replicon of Lactococcus lactis subsp. lactis UC317 Plasmid pCI305

    OpenAIRE

    Hayes, Finbarr; Daly, Charles; Fitzgerald, Gerald F.

    1990-01-01

    Replication functions of the stable, cryptic 8.7-kilobase (kb) plasmid pCI305 from multi-plasmid-containing Lactococcus lactis subsp. lactis UC317 were studied. Analysis of this replicon was facilitated by the construction of replication probe vectors that consisted of the pBR322 replication region, a pUC18-derived multiple cloning site, and either the cat gene of pC194 (pCI341; 3.1 kb) or the erm gene of pAMβ1 (pCI3330; 4.0 kb). Plasmid pCI305 was introduced into plasmid-free L. lactis subsp...

  18. Mdt(A), a New Efflux Protein Conferring Multiple Antibiotic Resistance in Lactococcus lactis and Escherichia coli

    OpenAIRE

    Perreten, Vincent; Schwarz, Franziska V.; Teuber, Michael; Levy, Stuart B.

    2001-01-01

    The mdt(A) gene, previously designated mef214, from Lactococcus lactis subsp. lactis plasmid pK214 encodes a protein [Mdt(A) (multiple drug transporter)] with 12 putative transmembrane segments (TMS) that contain typical motifs conserved among the efflux proteins of the major facilitator superfamily. However, it also has two C-motifs (conserved in the fifth TMS of the antiporters) and a putative ATP-binding site. Expression of the cloned mdt(A) gene decreased susceptibility to macrolides, lin...

  19. Glucose metabolism in Lactococcus lactis MG1363 under different aeration conditions: Requirement of acetate to sustain growth under microaerobic conditions

    DEFF Research Database (Denmark)

    Nordkvist, Mikkel; Jensen, N.B.S.; Villadsen, John

    2003-01-01

    Lactococcus lactis subsp. lactis MG1363 was grown in batch cultures on a defined medium with glucose as the energy source under different aeration conditions, namely, anaerobic conditions, aerobic conditions, and microaerobic conditions with a dissolved oxygen tension of 5% (when saturation with...... air was used as the reference). The maximum specific growth rate was high (0.78 to 0.91 h(-1)) under all aeration conditions but decreased with increasing aeration, and more than 90% of the glucose was converted to lactate. However, a shift in by-product formation was observed. Increasing aeration...

  20. Purification and characterization of a cell wall peptidase from Lactococcus lactis subsp. cremoris IMN-C12.

    OpenAIRE

    Sahlstrøm, S; J. Chrzanowska; Sørhaug, T

    1993-01-01

    A peptidase from the cell wall fraction of Lactococcus lactis subsp. cremoris IMN-C12 has been purified to homogeneity by hydrophobic interaction chromatography, two steps of anion-exchange chromatography, and gel filtration. The molecular mass of the purified enzyme was estimated to be 72 kDa by gel filtration and 23 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme has a pI of 4.0, and it has the following N-terminal sequence from the 2nd to the 17th amino acid re...

  1. Draft Genome Sequence of Lactococcus lactis subsp. lactis bv. diacetylactis CRL264, a Citrate-Fermenting Strain

    Science.gov (United States)

    Zuljan, Federico; Espariz, Martín; Blancato, Victor S.; Esteban, Luis; Alarcón, Sergio

    2016-01-01

    We report the draft genome sequence of Lactococcus lactis subsp. lactis bv. diacetylactis CRL264, a natural strain isolated from artisanal cheese from northwest Argentina. L. lactis subsp. lactis bv. diacetylactis is one of the most important microorganisms used as starter culture around the world. The CRL264 strain constitutes a model microorganism in the studies on the generation of aroma compounds (diacetyl, acetoin, and 2,3-butanediol) by lactic acid bacteria. Our genome analysis shows similar genetic organization to other available genomes of L. lactis bv. diacetylactis strains. PMID:26847906

  2. Characterization and overexpression of the Lactococcus lactis pepN gene and localization of its product, aminopeptidase N.

    OpenAIRE

    van Alen-Boerrigter, I J; Baankreis, R; de Vos, W M

    1991-01-01

    The chromosomal pepN gene encoding lysyl-aminopeptidase activity in Lactococcus lactis has been identified in a lambda EMBL3 library in Escherichia coli by using an immunological screening with antiserum against a purified aminopeptidase fraction. The pepN gene was localized and subcloned in E. coli on the basis of its expression and hybridization to a mixed-oligonucleotide probe for the previously determine N-terminal amino acid sequence of lysyl-aminopeptidase (P. S. T. Tan and W. N. Koning...

  3. The extracellular PI-type proteinase of Lactococcus lactis hydrolyzes beta-casein into more than one hundred different oligopeptides.

    OpenAIRE

    Juillard, V; van der Laan, H.; Kunji, E R; Jeronimus-Stratingh, C M; Bruins, A P; Konings, W. N.

    1995-01-01

    The peptides released from beta-casein by the action of PI-type proteinase (PrtP) from Lactococcus lactis subsp. cremoris Wg2 have been identified by on-line coupling of liquid chromatography to mass spectrometry. After 24 h of incubation of beta-casein with purified PrtP, a stable mixture of peptides was obtained. The trifluoroacetic acid-soluble peptides of this beta-casein hydrolysate were fractionated by high-performance liquid chromatography and introduced into the liquid chromatography-...

  4. Effect of Initial Oxygen Concentration on Diacetyl and Acetoin Production by Lactococcus lactis subsp. lactis biovar diacetylactis

    OpenAIRE

    Bassit, Naïma; Boquien, Clair-Yves; Picque, Daniel; Corrieu, Georges

    1993-01-01

    The production of aroma compounds (acetoin and diacetyl) in fresh unripened cheese by Lactococcus lactis subsp. lactis biovar diacetylactis CNRZ 483 was studied at 30°C at different initial oxygen concentrations (0, 21, 50, and 100% of the medium saturation by oxygen). Regardless of the initial O2 concentration, maximal production of these compounds was reached only after all the citrate was consumed. Diacetyl and acetoin production was 0.01 and 2.4 mM, respectively, at 0% oxygen. Maximum ace...

  5. Importance of bacterial endotoxin (LPS in endodontics A importância da endotoxina bacteriana (LPS na endodontia atual

    Directory of Open Access Journals (Sweden)

    Mario Roberto Leonardo

    2004-06-01

    Full Text Available New knowledge of the structure and biological activity of endotoxins (LPS has revolutionized concepts concerning their mechanisms of action and forms of inactivation. Since the 1980's, technological advances in microbiological culture and identification have shown that anaerobic microorganisms, especially Gram-negative, predominate in root canals of teeth with pulp necrosis and radiographically visible chronic periapical lesions. Gram-negative bacteria not only have different factors of virulence and generate sub-products that are toxic to apical and periapical tissues, as also contain endotoxin (LPS on their cell wall. This is especially important because endotoxin is released during multiplication or bacterial death, causing a series of biological effects that lead to an inflammatory reaction and resorption of mineralized tissues. Thus, due to the role of endotoxin in the pathogenesis of periapical lesions, we reviewed the literature concerning the biological activity of endotoxin and the relevance of its inactivation during treatment of teeth with pulp necrosis and chronic periapical lesion.O conhecimento mais aprofundado sobre a estrutura e atividade biológica das endotoxinas (LPS revolucionou os conceitos sobre seu mecanismo de ação e formas de inativação. A partir da década de 80, os avanços tecnológicos na cultura e identificação microbiológica demonstraram que, em canais radiculares de dentes portadores de necrose pulpar e lesão periapical crônica, visível radiograficamente, predominam microrganismos anaeróbios, particularmente os gram-negativos. Como se sabe, os microrganismos gram-negativos, além de possuírem diferentes fatores de virulência e gerarem produtos e sub-produtos tóxicos aos tecidos apicais e periapicais, contêm endotoxina em sua parede celular. Esse conhecimento é particularmente importante, uma vez que a endotoxina é liberada durante a multiplicação ou morte bacteriana, exercendo uma série de

  6. Miocarditis por dengue

    OpenAIRE

    Ricardo Amador García Hernández; Fidel Espinosa Rivera; Lianet Rivero Seriel

    2013-01-01

    El dengue es un arbovirus transmitido por el Aedes aegypti, produce los cuadros clínicos de dengue clásico, dengue hemorrágico y síndrome de choque por dengue, aisladamente se reportan casos de miocarditis. Se presentó un paciente con antecedentes de dengue clásico que manifestó dolor precordial asociado a cambios electrocardiográficos y fue ingresado en este hospital por síndrome coronario agudo probable, al cual se le diagnosticó clínicamente miocarditis por dengue.

  7. GABA Production in Lactococcus lactis Is Enhanced by Arginine and Co-addition of Malate.

    Science.gov (United States)

    Laroute, Valérie; Yasaro, Chonthicha; Narin, Waranya; Mazzoli, Roberto; Pessione, Enrica; Cocaign-Bousquet, Muriel; Loubière, Pascal

    2016-01-01

    Lactococcus lactis NCDO 2118 was previously selected for its ability to decarboxylate glutamate to γ-aminobutyric acid (GABA), an interesting nutritional supplement able to improve mood and relaxation. Amino acid decarboxylation is generally considered as among the biochemical systems allowing lactic acid bacteria to counteracting acidic stress and obtaining metabolic energy. These strategies also include arginine deiminase pathway and malolactic fermentation but little is known about their possible interactions of with GABA production. In the present study, the effects of glutamate, arginine, and malate (i.e., the substrates of these acid-resistance pathways) on L. lactis NCDO 2118 growth and GABA production performances were analyzed. Both malate and arginine supplementation resulted in an efficient reduction of acidity and improvement of bacterial biomass compared to glutamate supplementation. Glutamate decarboxylation was limited to narrow environmental conditions (pH < 5.1) and physiological state (stationary phase). However, some conditions were able to improve GABA production or activate glutamate decarboxylation system even outside of this compass. Arginine clearly stimulated glutamate decarboxylation: the highest GABA production (8.6 mM) was observed in cultures supplemented with both arginine and glutamate. The simultaneous addition of arginine, malate, and glutamate enabled earlier GABA production (i.e., during exponential growth) at relatively high pH (6.5). As far as we know, no previous study has reported GABA production in such conditions. Although further studies are needed to understand the molecular basis of these phenomena, these results represent important keys suitable of application in GABA production processes. PMID:27458444

  8. PpiA, a surface PPIase of the cyclophilin family in Lactococcus lactis.

    Directory of Open Access Journals (Sweden)

    Nicolas Trémillon

    Full Text Available BACKGROUND: Protein folding in the envelope is a crucial limiting step of protein export and secretion. In order to better understand this process in Lactococcus lactis, a lactic acid bacterium, genes encoding putative exported folding factors like Peptidyl Prolyl Isomerases (PPIases were searched for in lactococcal genomes. RESULTS: In L. lactis, a new putative membrane PPIase of the cyclophilin subfamily, PpiA, was identified and characterized. ppiA gene was found to be constitutively expressed under normal and stress (heat shock, H(2O(2 conditions. Under normal conditions, PpiA protein was synthesized and released from intact cells by an exogenously added protease, showing that it was exposed at the cell surface. No obvious phenotype could be associated to a ppiA mutant strain under several laboratory conditions including stress conditions, except a very low sensitivity to H(2O(2. Induction of a ppiA copy provided in trans had no effect i on the thermosensitivity of an mutant strain deficient for the lactococcal surface protease HtrA and ii on the secretion and stability on four exported proteins (a highly degraded hybrid protein and three heterologous secreted proteins in an otherwise wild-type strain background. However, a recombinant soluble form of PpiA that had been produced and secreted in L. lactis and purified from a culture supernatant displayed both PPIase and chaperone activities. CONCLUSIONS: Although L. lactis PpiA, a protein produced and exposed at the cell surface under normal conditions, displayed a very moderate role in vivo, it was found, as a recombinant soluble form, to be endowed with folding activities in vitro.

  9. Over-expressed CmbT multidrug resistance transporter improves the fitness of Lactococcus lactis

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    Filipić Brankica

    2013-01-01

    Full Text Available The influence of the over-expression of CmbT multidrug resistance transporter on the growth rate of Lactococcus lactis NZ9000 was studied. L. lactis is a lactic acid bacteria (LAB widely used as a starter culture in dairy industry. Recently characterized CmbT MDR transporter in L. lactis confers resistance to a wide variety of toxic compounds as well as to some clinically relevant antibiotics. In this study, the cmbT gene was over-expressed in the strain L. lactis NZ9000 in the presence of nisin inducer. Over-expression of the cmbT gene in L. lactis NZ9000 was followed by RT-PCR. The obtained results showed that the cmbT gene was successfully over-expressed by addition of sub-inhibitory amounts of nisin. Growth curves of L. lactis NZ9000/pCT50 over-expressing the cmbT gene and L. lactis NZ9000 control strain were followed in the rich medium as well as in the chemically defined medium in the presence solely of methionine (0.084 mM or mix of methionine and cysteine (8.4 mM and 8.2 mM, respectively. Resulting doubling times revealed that L. lactis NZ9000/pCT50 had higher growth rate comparing to the control strain. This could be a consequence of the CmbT efflux activity, which improves the fitness of the host bacterium through the elimination of toxic compounds from the cell.

  10. Food Safety: Secretome of Lactococcus lactis and Listeria monocytogenes in competition.

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    Isabella Alloggio

    2015-07-01

    Full Text Available Listeria monocytogenes (LM is a foodborne pathogen responsible of listeriosis. In the spreading of this pathology, milk and dairy products are key reservoir for this pathogen1. Food processing represents one of the major steps that could be linked to LM growth. Inhibition of LM growth through competition of Lactococcus lactis (LAC could represent a solution to this problem. Exoproteome of LM and two different strains of Lactic Acid Bacteria in co-culture have been studied in order to highlight mechanisms of bacterial competition useful to improve food safety. Two different strains of LAC and one strain of LM were cultivated in appropriate medium cultures (BHI, also in competition. Filtrated cultures (SECRETOME were lyophilized and resuspended for proteomics analysis. Shotgun analysis on each secretome was performed on nano UPLC-MS system. Obtained data reveal, during competition, the higher production by LM of moonlighting protein Enolase and Glucose 6 Phosphate isomerase, of Septation ring formation regulator EzrA, involved into cell replication and the lower secretion of Endopeptidase P60. In parallel, L. lactis produced higher amounts of Secreted 45 kDa protein and switched from lantibiotic Nisin A production to Nisin Z production. In competition with LM, LAC strain investigated produce higher amounts of Secreted 45 kDa protein with peptidoglycan lytic activity and the selective secretion of Nisin Z probably to improve lantibiotic solubility in less acidic environment. Next step will be validation of obtained results in dairy products. These results are of interesting to design new strategies of fighting LM as contaminant in food from animal origin.Work supported by Ministry of Health-CCM “Milano EXPO 2015 Project: Garantire la sicurezza alimentare- Valorizzare le produzioni”

  11. Coculture-inducible bacteriocin biosynthesis of different probiotic strains by dairy starter culture Lactococcus lactis

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    Blaženka Kos

    2011-12-01

    Full Text Available Bacteriocins produced by probiotic strains effectively contribute to colonization ability of probiotic strains and facilitate their establishment in the competitive gut environment and also protect the gut from gastrointestinal pathogens. Moreover, bacteriocins have received considerable attention due to their potential application as biopreservatives, especially in dairy industry. Hence, the objective of this research was to investigate antimicrobial activity of probiotic strains Lactobacillus helveticus M92, Lactobacillus plantarum L4 and Enterococcus faecium L3, with special focus on their bacteriocinogenic activity directed towards representatives of the same or related bacterial species, and towards distant microorganisms including potential food contaminants or causative agents of gut infections. In order to induce bacteriocin production, probiotic cells were cocultivated with Lactococcus lactis subsp. lactis LMG 9450, one of the most important starter cultures in cheese production. The presence of bacteriocin coding genes was investigated by PCR amplification with sequence-specific primers for helveticin and was confirmed for probiotic strain L. helveticus M92. All examined probiotic strains have shown bacteriocinogenic activity against Staphylococcus aureus 3048, Staphylococcus aureus K-144, Escherichia coli 3014, Salmonella enterica serovar Typhimurium FP1, Bacillus subtilis ATCC 6633, Bacillus cereus TM2, which is an important functional treat of probiotic strains significant in competitive exclusion mechanism which provides selective advantage of probiotic strains against undesirable microorganisms in gastrointestinal tract of the host. According to obtained results, living cells of starter culture Lc. lactis subsp. lactis LMG 9450 induced bacteriocin production by examined probiotic strains but starter culture itself was not sensitive to bacteriocin activity.

  12. Expanding the molecular toolbox for Lactococcus lactis: construction of an inducible thioredoxin gene fusion expression system

    LENUS (Irish Health Repository)

    Douillard, Francois P

    2011-08-09

    Abstract Background The development of the Nisin Inducible Controlled Expression (NICE) system in the food-grade bacterium Lactococcus lactis subsp. cremoris represents a cornerstone in the use of Gram-positive bacterial expression systems for biotechnological purposes. However, proteins that are subjected to such over-expression in L. lactis may suffer from improper folding, inclusion body formation and\\/or protein degradation, thereby significantly reducing the yield of soluble target protein. Although such drawbacks are not specific to L. lactis, no molecular tools have been developed to prevent or circumvent these recurrent problems of protein expression in L. lactis. Results Mimicking thioredoxin gene fusion systems available for E. coli, two nisin-inducible expression vectors were constructed to over-produce various proteins in L. lactis as thioredoxin fusion proteins. In this study, we demonstrate that our novel L. lactis fusion partner expression vectors allow high-level expression of soluble heterologous proteins Tuc2009 ORF40, Bbr_0140 and Tuc2009 BppU\\/BppL that were previously insoluble or not expressed using existing L. lactis expression vectors. Over-expressed proteins were subsequently purified by Ni-TED affinity chromatography. Intact heterologous proteins were detected by immunoblotting analyses. We also show that the thioredoxin moiety of the purified fusion protein was specifically and efficiently cleaved off by enterokinase treatment. Conclusions This study is the first description of a thioredoxin gene fusion expression system, purposely developed to circumvent problems associated with protein over-expression in L. lactis. It was shown to prevent protein insolubility and degradation, allowing sufficient production of soluble proteins for further structural and functional characterization.

  13. Interaction between the genomes of Lactococcus lactis and phages of the P335 species.

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    William John Kelly

    2013-08-01

    Full Text Available Phages of the P335 species infect Lactococcus lactis and have been particularly studied because of their association with strains of L. lactis subsp. cremoris used as dairy starter cultures. Unlike other lactococcal phages, those of the P335 species may have a temperate or lytic lifestyle, and are believed to originate from the starter cultures themselves. We have sequenced the genome of L. lactis subsp. cremoris KW2 isolated from fermented corn and found that it contains an integrated P335 species prophage. This 41 kb prophage (ΦKW2 has a mosaic structure with functional modules that are highly similar to several other phages of the P335 species associated with dairy starter cultures. Comparison of the genomes of 26 phages of the P335 species, with either a lytic or temperate lifestyle, shows that they can be divided into three groups and that the morphogenesis gene region is the most conserved. Analysis of these phage genomes in conjunction with the genomes of several L. lactis strains shows that prophage insertion is site specific and occurs at seven different chromosomal locations. Exactly how induced or lytic phages of the P335 species interact with carbohydrate cell surface receptors in the host cell envelope remains to be determined. Genes for the biosynthesis of a variable cell surface polysaccharide and for lipoteichoic acids are found in L. lactis and are the main candidates for phage receptors, as the genes for other cell surface carbohydrates have been lost from dairy starter strains. Overall, phages of the P335 species appear to have had only a minor role in the adaptation of L. lactis subsp. cremoris strains to the dairy environment, and instead they appear to be an integral part of the L. lactis chromosome. There remains a great deal to be discovered about their role, and their contribution to the evolution of the bacterial genome.

  14. Some chemical and physical properties of nisin, a small-protein antibiotic produced by Lactococcus lactis.

    Science.gov (United States)

    Liu, W; Hansen, J N

    1990-08-01

    Nisin is a small gene-encoded antimicrobial protein produced by Lactococcus lactis that contains unusual dehydroalanine and dehydrobutyrine residues. The reactivity of these residues toward nucleophiles was explored by reacting nisin with a variety of mercaptans. The kinetics of reaction with 2-mercaptoethane-sulfonate and thioglycolate indicated that the reaction pathway includes a binding step. Reaction of nisin at high pH resulted in the formation of multimeric products, apparently as a result of intramolecular and intermolecular reactions between nucleophilic groups and the dehydro residues. One of the nucleophiles had a pKa of about 9.8. The unique vinyl protons of the dehydro residues that give readily identifiable proton nuclear magnetic resonances were used to observe the addition of nucleophiles to the dehydro moiety. After reaction with nucleophiles, nisin lost its antibiotic activity and no longer showed the dehydro resonances, indicating that the dehydro groups had been modified. The effect of pH on the solubility of nisin was determined; the solubility was quite high at low pH (57 mg/ml at pH 2) and was much lower at high pH (0.25 mg/ml at pH 8 to 12), as measured before significant pH-induced chemical modification had occurred. High-performance liquid chromatography on a C18 column was an effective technique for separating unmodified nisin from its reaction products. The cyanogen bromide cleavage products of nisin were about 90% less active toward inhibition of bacterial spore outgrowth than was native nisin. These results are consistent with earlier observations, which suggested that the dehydro residues of nisin have a role in the mechanism of antibiotic action, in which they act as electrophilic Michael acceptors toward nucleophiles in the cellular target. PMID:2119570

  15. Transcriptome profiling of Lactococcus lactis subsp. cremoris CECT 8666 in response to agmatine.

    Science.gov (United States)

    Del Rio, Beatriz; Redruello, Begoña; Martin, M Cruz; Fernandez, Maria; de Jong, Anne; Kuipers, Oscar P; Ladero, Victor; Alvarez, Miguel A

    2016-03-01

    The dairy strain Lactococcus lactis subsp. cremoris CECT 8666 (formerly GE2-14) synthesizes the biogenic amine putrescine from agmatine via the agmatine deiminase (AGDI) pathway [1]. The AGDI cluster of L. lactis is composed by five genes aguR, aguB, aguD, aguA and aguC. The last four genes are co-transcribed as a single policistronic mRNA forming the catabolic operon aguBDAC, which encodes the proteins necessary for agmatine uptake and its conversion into putrescine [1], [2]. The first gene of the cluster, aguR, encodes a transmembrane protein that functions as a one-component signal transduction system that senses the agmatine concentration of the medium and accordingly regulates the transcription of aguBDAC[2]. The catabolic operon aguBDAC is transcriptionally activated by agmatine [2] and transcriptionally regulated by carbon catabolite repression (CCR) via glucose, but not by other sugars such as lactose or galactose [1], [3]. On the contrary, the transcription of the aguR regulatory gene is not subject to CCR regulation [1], [3] nor is regulated by agmatine [2]. In this study we report the transcriptional profiling of L. lactis subsp. cremoris CECT 8666 grown in M17 medium with galactose (GalM17) as carbon source and supplemented with agmatine, compared to that of the strain grown in the same culture medium without agmatine. The transcriptional profiling data of agmatine-regulated genes were deposited in the Gene Expression Omnibus (GEO) database under Accession no. GSE74808. PMID:26981381

  16. Characterization of plasmids in a human clinical strain of Lactococcus garvieae.

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    Mónica Aguado-Urda

    Full Text Available The present work describes the molecular characterization of five circular plasmids found in the human clinical strain Lactococcus garvieae 21881. The plasmids were designated pGL1-pGL5, with molecular sizes of 4,536 bp, 4,572 bp, 12,948 bp, 14,006 bp and 68,798 bp, respectively. Based on detailed sequence analysis, some of these plasmids appear to be mosaics composed of DNA obtained by modular exchange between different species of lactic acid bacteria. Based on sequence data and the derived presence of certain genes and proteins, the plasmid pGL2 appears to replicate via a rolling-circle mechanism, while the other four plasmids appear to belong to the group of lactococcal theta-type replicons. The plasmids pGL1, pGL2 and pGL5 encode putative proteins related with bacteriocin synthesis and bacteriocin secretion and immunity. The plasmid pGL5 harbors genes (txn, orf5 and orf25 encoding proteins that could be considered putative virulence factors. The gene txn encodes a protein with an enzymatic domain corresponding to the family actin-ADP-ribosyltransferases toxins, which are known to play a key role in pathogenesis of a variety of bacterial pathogens. The genes orf5 and orf25 encode two putative surface proteins containing the cell wall-sorting motif LPXTG, with mucin-binding and collagen-binding protein domains, respectively. These proteins could be involved in the adherence of L. garvieae to mucus from the intestine, facilitating further interaction with intestinal epithelial cells and to collagenous tissues such as the collagen-rich heart valves. To our knowledge, this is the first report on the characterization of plasmids in a human clinical strain of this pathogen.

  17. Transcriptome profiling of Lactococcus lactis subsp. cremoris CECT 8666 in response to agmatine

    Directory of Open Access Journals (Sweden)

    Beatriz del Rio

    2016-03-01

    Full Text Available The dairy strain Lactococcus lactis subsp. cremoris CECT 8666 (formerly GE2-14 synthesizes the biogenic amine putrescine from agmatine via the agmatine deiminase (AGDI pathway [1]. The AGDI cluster of L. lactis is composed by five genes aguR, aguB, aguD, aguA and aguC. The last four genes are co-transcribed as a single policistronic mRNA forming the catabolic operon aguBDAC, which encodes the proteins necessary for agmatine uptake and its conversion into putrescine [1,2]. The first gene of the cluster, aguR, encodes a transmembrane protein that functions as a one-component signal transduction system that senses the agmatine concentration of the medium and accordingly regulates the transcription of aguBDAC [2]. The catabolic operon aguBDAC is transcriptionally activated by agmatine [2] and transcriptionally regulated by carbon catabolite repression (CCR via glucose, but not by other sugars such as lactose or galactose [1,3]. On the contrary, the transcription of the aguR regulatory gene is not subject to CCR regulation [1,3] nor is regulated by agmatine [2]. In this study we report the transcriptional profiling of L. lactis subsp. cremoris CECT 8666 grown in M17 medium with galactose (GalM17 as carbon source and supplemented with agmatine, compared to that of the strain grown in the same culture medium without agmatine. The transcriptional profiling data of agmatine-regulated genes were deposited in the Gene Expression Omnibus (GEO database under Accession no. GSE74808.

  18. Heterologous expression of Brucella abortus GroEL heat-shock protein in Lactococcus lactis

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    Langella Philippe

    2006-03-01

    Full Text Available Abstract Background Brucella abortus is a facultative intracellular pathogen that mainly infects cattle and humans. Current vaccines rely on live attenuated strains of B. abortus, which can revert to their pathogenic status and thus are not totally safe for use in humans. Therefore, the development of mucosal live vaccines using the food-grade lactic acid bacterium, Lactococcus lactis, as an antigen delivery vector, is an attractive alternative and a safer vaccination strategy against B. abortus. Here, we report the construction of L. lactis strains genetically modified to produce B. abortus GroEL heat-shock protein, a candidate antigen, in two cellular locations, intracellular or secreted. Results Only the secreted form of GroEL was stably produced in L. lactis, suggesting a detrimental effect of GroEL protein when intracellularly produced in this bacterium. Only trace amounts of mature GroEL were detected in the supernatant fraction of induced lactococcal cultures, and the GroEL precursor remained stacked in the cell fraction. Attempts to raise the secretion yields were made, but even when GroEL was fused to a synthetic propeptide, secretion of this antigen was not improved. Conclusion We found that L. lactis is able to produce, and to secrete, a stable form of GroEL into the extracellular medium. Despite the low secretion efficiency of GroEL, which suggest that this antigen interacts with the cell envelope of L. lactis, secretion seems to be the best way to achieve both production and protein yields, regardless of cellular location. The L. lactis strain secreting GroEL has potential for in vivo immunization.

  19. Optimization of the Lactococcus lactis nisin-controlled gene expression system NICE for industrial applications

    Directory of Open Access Journals (Sweden)

    Mond James

    2005-05-01

    Full Text Available Abstract Background The nisin-controlled gene expression system NICE of Lactococcus lactis is one of the most widely used expression systems in Gram-positive bacteria. Despite its widespread use, no optimization of the culture conditions and nisin induction has been carried out to obtain maximum yields. As a model system induced production of lysostaphin, an antibacterial protein (mainly against Staphylococcus aureus produced by S. simulans biovar. Staphylolyticus, was used. Three main areas need optimization for maximum yields: cell density, nisin-controlled induction and protein production, and parameters specific for the target-protein. Results In a series of pH-controlled fermentations the following parameters were optimized: pH of the culture, use of NaOH or NH4OH as neutralizing agent, the addition of zinc and phosphate, the fermentation temperature, the time point of induction (cell density of the culture, the amount of nisin added for induction and the amount of three basic medium components, i.e. yeast extract, peptone and lactose. For each culture growth and lysostaphin production was followed. Lysostaphin production yields depended on all parameters that were varied. In the course of the optimization a three-fold increase in lysostaphin yield was achieved from 100 mg/l to 300 mg/l. Conclusion Protein production with the NICE gene expression system in L. lactis strongly depends on the medium composition, the fermentation parameters and the amount of nisin added for induction. Careful optimization of key parameters lead to a significant increase in the yield of the target protein.

  20. Fate of Lactococcus lactis starter cultures during late ripening in cheese models.

    Science.gov (United States)

    Ruggirello, Marianna; Cocolin, Luca; Dolci, Paola

    2016-10-01

    The presence of Lactococcus lactis, commonly employed as starter culture, was, recently, highlighted and investigated during late cheese ripening. Thus, the main goal of the present study was to assess the persistence and viability of this microorganism throughout manufacturing and ripening of model cheeses. Eight commercial starters, constituted of L. lactis subsp. lactis and L. lactis subsp. cremoris, were inoculated in pasteurized milk in order to manufacture miniature cheeses, ripened for six months. Samples were analysed at different steps (milk after inoculum, curd after cutting, curd after pressing and draining, cheese immediately after salting and cheese at 7, 15, 30, 60, 90, 120, 150 and 180 days of ripening) and submitted to both culture-dependent (traditional plating on M17) and -independent analysis (reverse transcription-quantitative PCR). On the basis of direct RNA analysis, L. lactis populations were detected in all miniature cheeses up to the sixth month of ripening, confirming the presence of viable cells during the whole ripening process, including late stages. Noteworthy, L. lactis was detected by RT-qPCR in cheese samples also when traditional plating failed to indicate its presence. This discrepancy could be explain with the fact that lactococci, during ripening process, enter in a stressed physiological state (viable not culturable, VNC), which might cause their inability to grow on synthetic medium despite their viability in cheese matrix. Preliminary results obtained by "resuscitation" assays corroborated this hypothesis and 2.5% glucose enrichment was effective to recover L. lactis cells in VNC state. The capability of L. lactis to persist in late ripening, and the presence of VNC cells which are known to shift their catabolism to peptides and amino acids consumption, suggests a possible technological role of this microorganism in cheese ripening with a possible impact on flavour formation. PMID:27375251

  1. Kefir-isolated Lactococcus lactis subsp. lactis inhibits the cytotoxic effect of Clostridium difficile in vitro.

    Science.gov (United States)

    Bolla, Patricia Araceli; Carasi, Paula; Serradell, María de los Angeles; De Antoni, Graciela Liliana

    2013-02-01

    Kefir is a dairy product obtained by fermentation of milk with a complex microbial population and several health-promoting properties have been attributed to its consumption. In this work, we tested the ability of different kefir-isolated bacterial and yeast strains (Lactobacillus kefir, Lb. plantarum, Lactococcus lactis subps. lactis, Saccharomyces cerevisiae and Kluyveromyces marxianus) or a mixture of them (MM) to antagonise the cytopathic effect of toxins from Clostridium difficile (TcdA and TcdB). Cell detachment assays and F-actin network staining using Vero cell line were performed. Although incubation with microbial cells did not reduce the damage induced by C. difficile spent culture supernatant (SCS), Lc. lactis CIDCA 8221 and MM supernatants were able to inhibit the cytotoxicity of SCS to Vero cells. Fraction of Lc. lactis CIDCA 8221 supernatant containing components higher than 10 kDa were responsible for the inhibitory activity and heating of this fraction for 15 min at 100 °C completely abrogated this ability. By dot-blot assay with anti-TcdA or anti-TcdB antibodies, concentration of both toxins seems to be reduced in SCS treated with Lc. lactis CIDCA 8221 supernatant. However, protective effect was not affected by treatment with proteases or proteases-inhibitors tested. In conclusion, we demonstrated that kefir-isolated Lc. lactis CIDCA 8221 secreted heat-sensitive products able to protect eukaryotic cells from cytopathic effect of C. difficile toxins in vitro. Our findings provide new insights into the probiotic action of microorganisms isolated from kefir against virulence factors from intestinal pathogens. PMID:23217732

  2. Nisin Z Production by Lactococcus lactis subsp. cremoris WA2-67 of Aquatic Origin as a Defense Mechanism to Protect Rainbow Trout (Oncorhynchus mykiss, Walbaum) Against Lactococcus garvieae.

    Science.gov (United States)

    Araújo, Carlos; Muñoz-Atienza, Estefanía; Pérez-Sánchez, Tania; Poeta, Patrícia; Igrejas, Gilberto; Hernández, Pablo E; Herranz, Carmen; Ruiz-Zarzuela, Imanol; Cintas, Luis M

    2015-12-01

    Probiotics represent an alternative to chemotherapy and vaccination to control fish diseases, including lactococcosis caused by Lactococcus garvieae. The aims of this study were (i) to determine the in vitro probiotic properties of three bacteriocinogenic Lactococcus lactis subsp. cremoris of aquatic origin, (ii) to evaluate in vivo the ability of L. cremoris WA2-67 to protect rainbow trout (Oncorhynchus mykiss, Walbaum) against infection by L. garvieae, and (iii) to demonstrate the role of nisin Z (NisZ) production as an anti-infective mechanism. The three L. cremoris strains survived in freshwater at 18 °C for 7 days, withstood exposure to pH 3.0 and 10 % (v/v) rainbow trout bile, and showed different cell surface hydrophobicity (37.93-58.52 %). The wild-type NisZ-producer L. cremoris WA2-67 and its non-bacteriocinogenic mutant L. cremoris WA2-67 ∆nisZ were administered orally (10(6) CFU/g) to rainbow trout for 21 days and, subsequently, fish were challenged with L. garvieae CLG4 by the cohabitation method. The fish fed with the bacteriocinogenic strain L. cremoris WA2-67 reduced significantly (p fish farming to prevent lactococcosis in rainbow trout. PMID:26307018

  3. Meningitis por Kluyvera sp. en una paciente con una derivación lumbo-peritoneal: reporte de un caso

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    Danny Paredes-Rodríguez

    2002-09-01

    Full Text Available Describimos el caso de una paciente portadora de una derivación lumbo- peritoneal, que desarrolló una meningitis bacteriana en la cual Kluyvera sp. se aisló del líquido cefalorraquídeo. En nuestro conocimiento este es el primer caso reportado de infección del SNC por este microorganismo. Esta bacteria era sensible a cefalosporinas de tercera generación y se logró la resolución con Cefotaxime. El Género Kluyvera comprende tres especies infrecuentemente aisladas de muestras clínicas. Se puede asociar a infecciones desde leves a severas y posiblemente se favorece en un huésped inmunocomprometido.

  4. Induction of antigen-specific tolerance by oral administration of Lactococcus lactis delivered immunodominant DQ8-restricted gliadin peptide in sensitized nonobese diabetic Abo Dq8 transgenic mice

    NARCIS (Netherlands)

    I.L. Huibregtse; E.V. Marietta; S. Rashtak; F. Koning; P. Rottiers; C.S. David; S.J.H. van Deventer; J.A. Murray

    2009-01-01

    Active delivery of recombinant autoantigens or allergens at the intestinal mucosa by genetically modified Lactococcus lactis (LL) provides a novel therapeutic approach for the induction of tolerance. Celiac disease is associated with either HLA-DQ2- or HLA-DQ8-restricted responses to specific antige

  5. How to distinguish between the vacuum cleaner and flippase mechanisms of the LmrA multi-drug transporter in Lactococcus lactis

    NARCIS (Netherlands)

    Hofmeyr, JHS; Rohwer, JM; Snoep, JL; Westerhoff, HV; Konings, WN

    2002-01-01

    A numerical model of the LmrA multi-drug transport system of Lactococcus lactis is used to explore the possibility of distinguishing experimentally between two putative transport mechanisms, i.e., the vacuum-cleaner and the flippase mechanisms. This comparative model also serves as an example of num

  6. Use of non-growing Lactococcus lactis cell suspensions for production of volatile metabolites with direct relevance for flavour formation during dairy fermentations

    OpenAIRE

    Bunt, van de, H.G.; Bron, P.A.; Sijtsma, L.; Vos, de, W.M.; Hugenholtz, J

    2014-01-01

    Abstract Background Lactococcus lactis is a lactic acid bacterium that has been used for centuries in the production of a variety of cheeses, as these bacteria rapidly acidify milk and greatly contribute to the flavour of the fermentation end-products. After a short growth phase during cheese ripening L. lactis enters an extended non-growing state whilst s...

  7. Differential expression of proteins and genes in the lag phase of Lactococcus lactis subsp lactis grown in synthetic medium and reconstituted skim milk

    DEFF Research Database (Denmark)

    Larsen, N.; Boye, Mette; Jakobsen, Marianne;

    2006-01-01

    We investigated protein and gene expression in the lag phase of Lactococcus lactis subsp. lactis CNRZ 157 and compared it to the exponential and stationary phases. By means of two-dimensional polyacrylamide gel electrophoresis, 28 highly expressed lag-phase proteins, implicated in nucleotide meta...

  8. Complete Genome Sequence of Lactococcus lactis IO-1, a Lactic Acid Bacterium That Utilizes Xylose and Produces High Levels of l-Lactic Acid

    OpenAIRE

    Kato, Hiroaki; Shiwa, Yuh; Oshima, Kenshiro; Machii, Miki; Araya-Kojima, Tomoko; Zendo, Takeshi; Shimizu-Kadota, Mariko; Hattori, Masahira; Sonomoto, Kenji; Yoshikawa, Hirofumi

    2012-01-01

    We report the complete genome sequence of Lactococcus lactis IO-1 (= JCM7638). It is a nondairy lactic acid bacterium, produces nisin Z, ferments xylose, and produces predominantly l-lactic acid at high xylose concentrations. From ortholog analysis with other five L. lactis strains, IO-1 was identified as L. lactis subsp. lactis.

  9. Cold Shock Proteins of Lactococcus lactis MG1363 Are Involved in Cryoprotection and in the Production of Cold-Induced Proteins

    NARCIS (Netherlands)

    Wouters, Jeroen A.; Frenkiel, Hélène; Vos, Willem M. de; Kuipers, Oscar P.; Abee, Tjakko

    2001-01-01

    Members of the group of 7-kDa cold-shock proteins (CSPs) are the proteins with the highest level of induction upon cold shock in the lactic acid bacterium Lactococcus lactis MG1363. By using double-crossover recombination, two L. lactis strains were generated in which genes encoding CSPs are disrupt

  10. Colonização e translocação bacteriana orofaríngea, gástrica e traqueal em crianças submetidas à ventilação pulmonar mecânica Colonización y translocación bacteriana orofaríngea, gástrica y traqueal en niños sometidos a ventilación pulmonar mecánica Oropharyngeal colonization, and gastric and tracheal bacterial translocation, in children experiencing mechanical ventilation

    Directory of Open Access Journals (Sweden)

    Denise Miyuki Kusahara

    2012-01-01

    Full Text Available OBJETIVO: Descrever o padrão de colonização e translocação bacteriana orofaríngea, gástrica e traqueal em crianças submetidas à ventilação pulmonar mecânica. MÉTODOS: Estudo descritivo, realizado em uma Unidade de Cuidados Intensivos Pediátricos. Admitiram-se no estudo 30 crianças, sendo analisadas 216 culturas seriadas de secreção orofaríngea, gástrica e traqueal. Características microbiológicas, demográficas, clínicas, e terapêuticas foram avaliadas. RESULTADOS: Houve predominância de crianças portadoras de doenças crônicas, que fizeram uso de antibióticos, sedativos e protetores gástricos, submetidas à sondagem gástrica. Houve aumento no número de crianças colonizadas por patógenos durante a internação e predomínio das espécies: Enterobacter spp, K.pneumoniae, P.aeruginosa, A. baumanii e S.aureus. A maioria das crianças (80,0% sofreu translocação orofaríngea durante a internação na UCIP. CONCLUSÃO: Crianças criticamente enfermas podem representar grupo de pacientes com risco aumentado para colonização e translocação bacteriana predominantemente da região orofaríngea para a traquéia.OBJETIVO: Describir el patrón de colonización y translocación bacteriana orofaríngea, gástrica y traqueal en niños sometidos a ventilación pulmonar mecánica. MÉTODOS: Estudio descriptivo, realizado en una Unidad de Cuidados Intensivos Pediátricos. Se admitieron en el estudio a 30 niños, siendo analizados 216 cultivos seriados de secreción orofaríngea, gástrica y traqueal. Fueron evaluadas características microbiológicas, demográficas, clínicas, y terapéuticas. RESULTADOS: Hubo predominio de niños portadores de enfermedades crónicas, que hicieron uso de antibióticos, sedantes y protectores gástricos, sometidos a sondaje gástrico. Hubo aumento en el número de niños colonizados por patógenos durante el internamiento y predominio de las especies: Enterobacter spp, K.pneumoniae, P

  11. Efeito do cultivo da soja na dinâmica da população bacteriana, em solos de cerrado Effects of soybean cultivation on the bacterial population dynamics in cerrado soils

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    JOÃO CARLOS PEREIRA

    2000-06-01

    Full Text Available Este trabalho teve por objetivo avaliar a influência do cultivo da soja sobre a dinâmica da população bacteriana, em dois solos de Cerrado do Estado de São Paulo, originalmente cobertos com Paspalum notatum (em Barretos e Brachiaria decumbens (em S��o Carlos. Nesses solos, a densidade da população de bactérias em geral variou de 398,1 x 10³ a 467,7 x 10³ e de 123 x 10³ a 218,8 x 10³ ufc (unidades formadoras de colônias/g de solo seco, respectivamente. O cultivo da soja, em ambos os solos, resultou em incrementos variados nos números de ufc/g de solo seco da população de bactérias em geral, das resistentes aos antibióticos estreptomicina e cloranfenicol, e de actinomicetos. A população de actinomicetos ocorreu no solo principalmente como esporos, e as variações das relações esporos/hifas entre os solos não-rizosférico e rizosférico não foram significativas. Os resultados evidenciam que o cultivo da soja influenciou de forma diferenciada a população desses solos.The effect of soybean cultivation on the population dynamics of the bacterial community was evaluated in two "Cerrado" soils of São Paulo State, Brazil. The experimental areas, in the vicinities of the cities of São Carlos and Barretos, were previously cultivated, respectively, with Paspalum notatum and Brachiaria decumbens. The bacterial population densities in these soils varied from 398.1 x 10³ to 467.7 x 10³ cfu (colony forming units and from 123 x 10³ to 218.8 x 10³ cfu/g of dried soil, respectively, in São Carlos and Barretos soils. Soybean cultivation in both soils resulted in increments in the total bacterial population density, in the actinomycetes population, and in the bacterial population resistant to the antibiotics streptomycin and chloramphenicol. Actinomycetes were present in these soils mainly as spores. Soybean cultivation did not alter the actinomycetes spores/hyphae ratio when comparing rhizospheric and non-rhizospheric soils

  12. Bacterial survival rate on toothbrushes and their decontamination with antimicrobial solutions Taxa de sobrevivência bacteriana em escovas dentais e sua descontaminação com soluções antimicrobianas

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    Sandra Sato

    2004-06-01

    Full Text Available The purpose of this study was to evaluate bacterial survival rate on toothbrushes after brushing and the efficacy of their decontamination by spraying antimicrobial solutions. Thirty subjects were instructed to spray the solutions on toothbrush bristles after brushing. Each volunteer tested three sprays, one solution per week; the sprays were labeled spray 1 (cetylpyridinium chloride - CPC - and basic formulation, 2 (basic formulation only and 3 (control - sterile tap water. At the end of each week, the brushes were collected and sonicated in Letheen Broth®; the suspensions were ten-fold diluted and the dilutions were plated on various culture media. Anaerobic bacteria, evaluated by colony count of black pigment producing organisms on Ask medium, were recovered from 83.3% of the samples, Streptococci from 80% and aerobic Gram-negative bacilli from 46.7% of them in the control tests. There was a significant decrease in toothbrush contamination with antimicrobial sprays 1 and 2, the first showing the greatest decrease on bacterial counts.O propósito deste estudo foi avaliar a taxa de sobrevivência bacteriana em escovas dentais após a escovação e a eficácia na sua descontaminação pelo borrifamento de soluções antimicrobianas. Trinta indivíduos foram instruídos a borrifar as soluções nas cerdas das escovas após a escovação. Cada voluntário testou três sprays, uma solução por semana; os sprays foram rotulados spray 1 (cloreto de cetilpiridínio - CCP - e formulação básica, 2 (formulação básica apenas e 3 (controle - água de torneira esterilizada. Ao final de cada semana, as escovas eram recolhidas e introduzidas no caldo Letheen®, submetidas a ultra-som, à diluição decimal seriada e as suspensões semeadas em vários meios de cultura. As bactérias anaeróbias, avaliadas pela contagem de colônias de microrganismos produtores de pigmento negro no meio Ask, foram recuperadas em 83,3% das amostras, estreptococos em 80

  13. Efeito da solarização e biofumigação, durante o outono, na incidência de murcha-bacteriana e produtividade da batata Effect of soil solarization and biofumigation during autumn on bacterial wilt incidence and potato yield

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    Mírian Josefina Baptista

    2006-03-01

    Full Text Available A murcha-bacteriana causada por Ralstonia solanacearum é uma das principais doenças da cultura da batata. A solarização tem sido estudada como opção para a desinfestação do solo e tem potencial para o controle da murcha bacteriana. A técnica é indicada para uso nas estações quentes do ano pois depende de condições climáticas adequadas. Devido ao período de plantio de determinadas culturas, é interessante avaliar o uso da solarização em outras épocas do ano e associada a outras técnicas para garantir sua eficiência. A biofumigação é a desinfestação do solo através da adição de matéria orgânica que, durante sua decomposição, libera substâncias tóxicas aos fitopatógenos. Neste trabalho avaliou-se durante o outono (maio a junho os efeitos da adição de cama de aves (biofumigação e da solarização na incidência natural da murcha-bacteriana e na produtividade da batata, através dos tratamentos: adição de cama de aves (20 t/ha, uréia (100 kg/ha, aplicação de brometo de metila e solo sem tratamento (testemunha, todos solarizados ou não solarizados. Avaliou-se a produção de tubérculos totais e comerciais e, a partir da incidência de murcha-bacteriana, foi feito o cálculo da área abaixo da curva de progresso da doença (AACPD. Na área infestada, apenas o uso do brometo de metila proporcionou reduções significativas na incidência da murcha-bacteriana. No entanto, a aplicação de brometo de metila e a adição de cama de aves em associação com a solarização possibilitaram produção significativamente maior de tubérculos de batata em relação à testemunha. Os efeitos da solarização associada à biofumigação com cama de aves sobre a produtividade da batata em área infestada com R. solanacearum devem ser melhor investigados.Bacterial wilt caused by Ralstonia solanacearum is one of the most important diseases of the potato. Soil solarization has been studied as an option for soilborne

  14. Relación placa bacteriana y caries en un grupo de niños escolarizados entre 5 y 14 años de la población de la Boquilla - Cartagena en el año 2004-1

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    Lizelia Alfaro Zolá

    2013-10-01

    Full Text Available ResumenObjetivo: Describir la ocurrencia de placa bacteriana en las superficies dentales y relacionarla con el tipo de lesión de caries que se presenta en niños escolares de 5 a 14 años de la Boquilla - Cartagena. Materiales y Métodos: Se realizó un estudio de corte transversal, en el cual se seleccionó una muestra de 89 niños escolares de la población de la Boquilla a través de un muestreo aleatorio sistemático, a quienes se les evaluó el estado de higiene oral mediante el índice de placa comunitario de Corchuelo modificado y el índice de caries superficial con criterios de diagnóstico Ekstrand. Resultados: La superficie con lesiones de caries de mayor frecuencia fue la oclusal, presentándose un mayor numero de lesiones detenidas, seguido en palatino por las lesiones precavitacionales, a diferencia de las superficies vestibular y lingual donde se encontraron un mayor número de superficies sanas. En todas las superficies evaluadas predominó la presencia de placa bacteriana independientemente de la presencia de caries y no se encontró una relación estadísticamente significativa entre estas dos variables. Conclusiones: La alta frecuencia de placa bacteriana en las superficies dentarias nos mostró el deficiente estado de higiene oral de la población, sin embargo, sigue en duda la pertinencia de los índices de placa para la predicción de la caries debido a que no se encontró relación estadística. (Duazary 2007; 2: 119 - 126AbstractObjectives: describe the presence of dental plaque in healthy in the dental surfaces and relate it with the type of lesion of caries in children between 5 and 14 years of age in the village of la Boquilla- Cartagena. Material and Methods: This is study transversal, in a universe of 89 scholar children in the village of la Boquilla, 60 of these were chosen through a systematic aleatory sampling. They were evaluated to establish their status of oral hygiene using the modified

  15. Endoftalmites bacterianas com culturas positivas: uma revisão de 6 anos Culture proven bacterial endophthalmitis: a 6-year review

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    Paulo José Martins Bispo

    2008-10-01

    Full Text Available OBJETIVO: Determinar a distribuição dos microrganismos isolados de pacientes com endoftalmite bacteriana e sua sensibilidade a antimicrobianos. MÉTODOS: Foram analisados retrospectivamente os dados clínicos e microbiológicos dos pacientes com hipótese diagnóstica de endoftalmite e cultura bacteriana positiva, atendidos no Departamento de Oftalmologia da UNIFESP de 1º de janeiro de 2000 a 31 de dezembro de 2005. RESULTADOS: De 451 pacientes, 153 (33,9% apresentaram cultura bacteriana positiva. Foram isolados 155 microrganismos, sendo 79,35% gram-positivos e 20,65% gram-negativos. Os Staphylococcus coagulase-negativos (SCoN (41,94% foram os mais freqüentemente isolados. A sensibilidade aos antimicrobianos entre os gram-negativos foi: amicacina 87,10%, tobramicina 80,65%, ciprofloxacina 96,67%, levofloxacina, gatifloxacina e moxifloxacina 100%, ceftazidima 85%, e gentamicina 80,65%. A sensibilidade à vancomicina entre os gram-positivos foi de 100%. S. aureus e SCoN apresentaram 83,33% de sensibilidade à oxacilina, 89,61% à ciprofloxacina e 100% à gatifloxacina e moxifloxacina. A forma de aquisição predominante foi a pós-operatória (60,65%. CONCLUSÃO: Observamos baixa sensibilidade da cultura para o diagnóstico etiológico das endoftalmites. Uma terapia antimicrobiana ou profilaxia empírica deve ser ativa contra os microrganismos gram-positivos, particularmente contra estafilococos. Estudos de vigilância de resistência bacteriana são importantes para adequação desses esquemas.PURPOSE: To assess the distribution of microorganisms isolated from patients with bacterial endophthalmitis and their antimicrobial susceptibility. METHODS: Retrospective analysis of medical and microbiological records of patients with suspected diagnosis of endophthalmitis and bacterial culture-proven at the Department of Ophthalmology, UNIFESP, between January 1 2000 and December 31 2005. RESULTS: 153 (33.9% of 451 patients showed positive bacterial

  16. DESENVOLVIMENTO DE MODELO DE PERITONITE BACTERIANA PARA AVALIAÇÃO DO TRATAMENTO MEDIANTE ACESSO LAPAROTÔMICO E VIDEO-LAPAROSCÓPICO

    OpenAIRE

    Salgado Jr W.; Cunha FQ; Sankarankuty A.S.; JS Santos

    2001-01-01

    Introdução: O emprego do acesso videolaparoscópico no tratamento das afecções digestivas que cursam com peritonite generalizada é motivo de controvérsia. Objetivo: Desenvolver um modelo de peritonite bacteriana para avaliação do tratamento mediante acesso laparotômico e videolaparoscópico. Métodos: Ratos machos Wistar foram submetidos à ligadura de ceco (CLP) sob molde rígido de 3mm de diâmetro; na seqüência foram feitas 14 punções no ceco com agulha 15X10. Após 6 horas de indução da peritoni...

  17. Dinâmica das populações bacterianas em solos de Cerrados Dynamic of bacterial populations from Cerrado soils

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    João Carlos Pereira

    1999-05-01

    Full Text Available Nos ambientes tropicais, os Cerrados destacam-se pelo seu potencial agrícola. Apesar das funções dos microrganismos no crescimento das plantas e na produtividade das culturas, existem poucas informações dos efeitos resultantes do manejo do solo, na ecologia microbiana. Neste estudo, foram avaliados os efeitos das condições ambientais e das práticas agrícolas sobre as populações bacterianas. As densidades das populações em solos com vegetação nativa foram variáveis e diferenciadas. Em Sete Lagoas, MG, as populações de actinomicetos variaram de 1,7 a 50 X 10(4 UFC/g de solo seco, enquanto em Planaltina as densidades das populações bacterianas em solo com primeiro e segundo ano de cultivo de soja foram semelhantes, mas superiores ao solo com vegetação nativa. A utilização agrícola deste solo não resultou em desequilíbrios acentuados das populações de actinomicetos provenientes de esporos e hifas. As relações esporos/hifas variaram de 1,1 a 5,8. Na rizosfera da soja, os coeficientes de correlação entre as populações de actinomicetos com as demais populações bacterianas foram significativos. Os resultados evidenciam que as práticas agrícolas utilizadas na introdução da cultura da soja em solos de Cerrados pode influenciar o equilíbrio das populações na comunidade bacteriana.Among tropical environments, Cerrados stand out because of its agriculture potencial. Although microorganisms play an important role on soil sustainability and crop production, few information is available on the effects of soil management systems on Cerrado's microbial ecology. In this study the effects of environmental conditions and soil management practices on bacterial populations were evaluated. Bacterial population densities in soil under native vegetation were variable and diferentiated. Actinomycetes densities varied from 1.7 to 50 X 10(4 CFU/g dry soil in Sete Lagoas region, Brazil, whereas bacterial populations in both the

  18. Fine tuning of the lactate and diacetyl production through promoter engineering in Lactococcus lactis.

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    Tingting Guo

    Full Text Available Lactococcus lactis is a well-studied bacterium widely used in dairy fermentation and capable of producing metabolites with organoleptic and nutritional characteristics. For fine tuning of the distribution of glycolytic flux at the pyruvate branch from lactate to diacetyl and balancing the production of the two metabolites under aerobic conditions, a constitutive promoter library was constructed by randomizing the promoter sequence of the H(2O-forming NADH oxidase gene in L. lactis. The library consisted of 30 promoters covering a wide range of activities from 7,000 to 380,000 relative fluorescence units using a green fluorescent protein as reporter. Eleven typical promoters of the library were selected for the constitutive expression of the H(2O-forming NADH oxidase gene in L. lactis, and the NADH oxidase activity increased from 9.43 to 58.17-fold of the wild-type strain in small steps of activity change under aerobic conditions. Meanwhile, the lactate yield decreased from 21.15 ± 0.08 mM to 9.94 ± 0.07 mM, and the corresponding diacetyl production increased from 1.07 ± 0.03 mM to 4.16 ± 0.06 mM with the intracellular NADH/NAD(+ ratios varying from 0.711 ± 0.005 to 0.383 ± 0.003. The results indicated that the reduced pyruvate to lactate flux was rerouted to the diacetyl with an almost linear flux variation via altered NADH/NAD(+ ratios. Therefore, we provided a novel strategy to precisely control the pyruvate distribution for fine tuning of the lactate and diacetyl production through promoter engineering in L. lactis. Interestingly, the increased H(2O-forming NADH oxidase activity led to 76.95% lower H(2O(2 concentration in the recombinant strain than that of the wild-type strain after 24 h of aerated cultivation. The viable cells were significantly elevated by four orders of magnitude within 28 days of storage at 4°C, suggesting that the increased enzyme activity could eliminate H(2O(2 accumulation and prolong cell survival.

  19. High efficiency electrotransformation of Lactococcus lactis spp. lactis cells pretreated with lithium acetate and dithiothreitol

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    Filioussis George

    2007-03-01

    Full Text Available Abstract Background A goal for the food industry has always been to improve strains of Lactococcus lactis and stabilize beneficial traits. Genetic engineering is used extensively for manipulating this lactic acid bacterium, while electropolation is the most widely used technique for introducing foreign DNA into cells. The efficiency of electrotransformation depends on the level of electropermealization and pretreatment with chemicals which alter cell wall permeability, resulting in improved transformation efficiencies is rather common practice in bacteria as in yeasts and fungi. In the present study, treatment with lithium acetate (LiAc and dithiothreitol (DTT in various combinations was applied to L. lactis spp. lactis cells of the early-log phase prior to electroporation with plasmid pTRKH3 (a 7.8 kb shuttle vector, suitable for cloning into L. lactis. Two strains of L. lactis spp. lactis were used, L. lactis spp. lactis LM0230 and ATCC 11454. To the best of our knowledge these agents have never been used before with L. lactis or other bacteria. Results Electrotransformation efficiencies of up to 105 transformants per μg DNA have been reported in the literature for L. lactis spp.lactis LM0230. We report here that treatment with LiAc and DDT before electroporation increased transformation efficiency to 225 ± 52.5 × 107 transformants per μg DNA, while with untreated cells or treated with LiAc alone transformation efficiency approximated 1.2 ± 0.5 × 105 transformants per μg DNA. Results of the same trend were obtained with L. lactis ATCC 11454, although transformation efficiency of this strain was significantly lower. No difference was found in the survival rate of pretreated cells after electroporation. Transformation efficiency was found to vary directly with cell density and that of 1010 cells/ml resulted in the highest efficiencies. Following electrotransformation of pretreated cells with LiAc and DDT, pTRKH3 stability was examined

  20. Immunogenicity and immunoprotection of porcine circovirus type 2 (PCV2) Cap protein displayed by Lactococcus lactis.

    Science.gov (United States)

    Li, Peng-cheng; Qiao, Xu-wen; Zheng, Qi-sheng; Hou, Ji-bo

    2016-01-27

    The capsid (Cap) protein, an important immunoprotective protein of porcine circovirus type 2 (PCV2), was expressed on the cell surface of the Gram-positive food-grade bacterium, Lactococcus lactis. Cap protein was fused to the peptidoglycan binding domain (known as the protein anchor domain, PA) of the lactococcal AcmA cell-wall hydrolase. The Cap protein fusion was non-covalently rebound to the surface of non-genetically modified, non-living high-binder L. lactis cells (designated Gram-positive enhancer matrix (GEM) particles). Expression of the recombinant GEM-displaying capsid protein (GEM-PA-Cap) was verified by Western blotting and immunofluorescence and transmission electron microscopy assays. To evaluate the immunogenicity of the recombinant Cap protein (rCap), 20 PCV2-seronegative piglets were immunized with the GEM-PA-Cap subunit vaccine, GEM alone, or phosphate-buffered saline (PBS, challenge control and empty control). Each group consisted of five piglets. The results showed that the level of PCV2-specific antibodies in piglets immunized with the GEM-PA-Cap subunit vaccine was significantly higher than that of the piglets immunized with GEM alone or the control group at all the time points post-vaccination (P<0.01). After challenge with the PCV2 wild-type strain, piglets that received the GEM-PA-Cap subunit vaccine showed significantly higher average daily weight gain (DWG) and shorter fever duration than the other two groups (P<0.001). Furthermore, a significant reduction in the gross lung lesion scores and lymph node lesion scores was noted in the GEM-PA-Cap-immunized group compared with the scores of the GEM or PBS-treated group (P<0.01). The results suggest that recombinant rCap displayed by L. lactis GEM particles provided the piglets with significant immunoprotection from PCV2-associated disease. Thus, the novel GEM-PA-Cap subunit vaccine has potential to be considered an effective and safe candidate vaccine against PCV2 infection in piglets. PMID

  1. Avaliação e acompanhamento audiológico após meningite bacteriana Audiological assessment and follow-up post bacterial meningitis

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    MARIA INÊS VIEIRA COUTO

    1999-09-01

    Full Text Available A deficiência auditiva é uma das sequelas da meningite bacteriana que ocorre com maior frequência em crianças. Este estudo descreve o perfil audiológico (periférico e central de crianças internadas com diagnóstico de meningite bacteriana. Nas 89 crianças que compareceram ao seguimento audiológico após a alta hospitalar e foram submetidas aos testes audiológicos, os resultados evidenciaram que 85,4% apresentaram acuidade auditiva normal em ambas orelhas, 10,1% apresentaram deficiência auditiva neurossensorial bilateral e 4,5% apresentaram deficiência auditiva neurossensorial unilateral. Nos testes que avaliaram as habilidades de processamento auditivo, os resultados mostraram que 10% dessas crianças apresentaram alteração no desempenho de localização auditiva e de reconhecimento de sentenças com mensagem competitiva ipsilateral.Hearing loss is the more frequent sequel of bacterial meningitis in children. This study describes the audiological profile (peripheric and central of 89 children admitted to the hospital wards with the diagnosis of bacterial meningitis. Those children attended audiological follow up, after their hospital descharge, and were submitted to audiological tests. The results showed that 85.4% among them presented normal hearing in both ears 10.1% presented bilateral neurosensorial hearing loss and 4.5% presented unilateral neurosensorial hearing loss. The results from the auditory processing skills assessment showed that 10% of those children presented auditory localization and recognition of sentences with competitive messages (Paediatric Sentences Identification - ipsilateral disorders.

  2. Ação do biofertilizante Agrobio sobre a mancha-bacteriana e desenvolvimento de mudas de pimentão Effect of the Agrobio biofertilizer on the bacterial spot and the development of bell pepper transplants

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    Cláudia S.R. Deleito

    2005-03-01

    Full Text Available O biofertilizante Agrobio é produzido a partir de esterco bovino fresco, água, melaço e sais minerais em recipientes abertos. Tem sido largamente utilizado por agricultores orgânicos e convencionais no estado do Rio de Janeiro, ao qual atribuem efeito nutricional e de controle de doenças. O presente trabalho foi feito com o objetivo de elucidar e quantificar aspectos relativos ao efeito do Agrobio sobre o controle da mancha-bacteriana e desenvolvimento de mudas de pimentão, em condições de casa-de-vegetação. Utilizaram-se as cultivares Cascadura Ikeda e Cascadura Itaipu e os tratamentos à base de Agrobio, diluído a 5% em água destilada, previamente submetido aos seguintes preparos: original; autoclavado (120ºC/20 min; filtrado em millipore (0,22 mm de diâmetro e a fração retida no filtro millipore após a filtragem do produto, e dois modos de aplicação (foliar e no substrato e, como testemunhas oxitetraciclina + sulfato de estreptomicina (0,8 g L-1, oxicloreto de cobre (2,4 g L-1 e água. O Agrobio original e filtrado em aplicação foliar, em geral, proporcionaram melhor controle da doença e maior desenvolvimento das mudas que o Agrobio autoclavado ou a sua fração retida em aplicação via substrato. O controle da doença pelo Agrobio, foi inferior ao proporcionado pelos produtos comerciais à base de oxicloreto de cobre e de oxitetraciclina + sulfato de estreptomicina, porém foi estatisticamente superior à testemunha água. O Agrobio favoreceu, porém, o desenvolvimento vegetativo das mudas, o aumento da área foliar, a maior retenção das folhas infectadas e a população de Bacillus spp.The Agrobio biofertilizer is prepared from fresh cattle manure, water, molasses and minerals in open containers. This product has been widely used by organic and conventional farmers of the Rio de Janeiro State to control several plant pathogens. This research aimed to elucidate and quantify some of the aspects related to the

  3. Cuatro métodos de predicción de riesgo de infección bacteriana grave en recién nacidos febriles Four methods to predict the severe bacterial infection risk in the febrile newborns

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    Manuel Díaz Álvarez

    2010-12-01

    Full Text Available INTRODUCCIÓN. El objetivo de esta investigación fue contrastar la efectividad de 4 métodos de evaluación de riesgo de infección bacteriana grave (IBG en recién nacidos (RN febriles, uno de los cuales fue desarrollado por los autores del trabajo y los otros son los utilizados en la práctica internacional. MÉTODOS. Se realizó un estudio descriptivo, retrospectivo y aplicado de 1358 RN febriles sin signos de focalización evaluados y clasificados por riesgo de IBG. Fue aplicado el método propuesto por los autores, que comprende: antecedente de ser sano; ausencia de impresión médica de un estado tóxico-infeccioso; ausencia de evidencias de infección focal en el examen físico; fiebre menor de 39 ºC y no persistente o recurrente; recuento de leucocitos sanguíneos > 5,0 x 10(9/L y INTRODUCTION. The aim of present research was to contrast the effectiveness of four assessment methods of severe bacterial infection risk (SBI in febrile newborns (NB , one of above mentioned methods was developed by paper's authors and the other are used in the international practice. METHODS. A retrospective and descriptive study was conducted and applied in 1358 febrile NB with no focalization signs and classified according to the SBI. The method proposed by authors was applied including a history of be healthy, lack of medical criterion of a infectious-toxic state, lack of evidences of focal infection in physical examination, fever lower than 39°C and no persistent or recurrent, blood leucocytes count >5,0 x 10(9/L and < 20,0 x 10(9/L, globular sedimentation speed <20 mm/h and urine leucocyte count <10 000/mL. This method and the other were applied in a initial assessment and at the end of the evolution 48 h. The predictive values were estimated for the different methods and also the effectiveness was assessed according to the gain function. RESULTS. The SBI was of 20,4%. The more frequent cause was the urinary tract infection. From the 652 NB initially

  4. Engineering the cell surface display of cohesins for assembly of cellulosome-inspired enzyme complexes on Lactococcus lactis

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    Wieczorek Andrew S

    2010-09-01

    Full Text Available Abstract Background The assembly and spatial organization of enzymes in naturally occurring multi-protein complexes is of paramount importance for the efficient degradation of complex polymers and biosynthesis of valuable products. The degradation of cellulose into fermentable sugars by Clostridium thermocellum is achieved by means of a multi-protein "cellulosome" complex. Assembled via dockerin-cohesin interactions, the cellulosome is associated with the cell surface during cellulose hydrolysis, forming ternary cellulose-enzyme-microbe complexes for enhanced activity and synergy. The assembly of recombinant cell surface displayed cellulosome-inspired complexes in surrogate microbes is highly desirable. The model organism Lactococcus lactis is of particular interest as it has been metabolically engineered to produce a variety of commodity chemicals including lactic acid and bioactive compounds, and can efficiently secrete an array of recombinant proteins and enzymes of varying sizes. Results Fragments of the scaffoldin protein CipA were functionally displayed on the cell surface of Lactococcus lactis. Scaffolds were engineered to contain a single cohesin module, two cohesin modules, one cohesin and a cellulose-binding module, or only a cellulose-binding module. Cell toxicity from over-expression of the proteins was circumvented by use of the nisA inducible promoter, and incorporation of the C-terminal anchor motif of the streptococcal M6 protein resulted in the successful surface-display of the scaffolds. The facilitated detection of successfully secreted scaffolds was achieved by fusion with the export-specific reporter staphylococcal nuclease (NucA. Scaffolds retained their ability to associate in vivo with an engineered hybrid reporter enzyme, E. coli β-glucuronidase fused to the type 1 dockerin motif of the cellulosomal enzyme CelS. Surface-anchored complexes exhibited dual enzyme activities (nuclease and β-glucuronidase, and were

  5. Biofertilizante agrobio: Uma alternativa no controle da mancha bacteriana em mudas de pimentão (Capsicum annuum l.)

    OpenAIRE

    Deleito, Cláudia Sayão Ramirez; Carmo, Margarida Goréte Ferreira do; Fernandes, Maria do Carmo de Araújo; Abboud, Antônio Carlos de Souza

    2004-01-01

    Agrobio é um biofertilizante líquido fabricado à base de esterco bovino, água, melaço e sais minerais, que são submetidos a um processo de fermentação à temperatura ambiente por 56 dias em recipientes abertos. Este produto tem sido largamente utilizado com sucesso por agricultores orgânicos e convencionais em todo o estado do Rio de Janeiro, no controle de várias enfermidades vegetais em diferentes culturas. Em bioensaios realizados em casa de vegetação, observou-se uma ação bacteriostática e...

  6. Transfer of nisin gene cluster from Lactococcus lactis ATCC 11454 into the chromosome of Bacillus subtilis 168.

    Science.gov (United States)

    Yuksel, Sahru; Hansen, J Norman

    2007-03-01

    Nisin is an antimicrobial peptide produced by certain strains of Lactococcus lactis. It is a gene-encoded peptide that contains unusual amino acid residues. These novel residues are introduced by posttranslational modification machinery and confer unique chemical and physical properties that are not attainable by regular amino acid residues. To study the modification mechanisms and to create structural analogs with superior properties, it would be advantageous to insert the nisin genes into a bacterial strain that is amenable to genetic manipulation. In this study, we report the cloning and integration of the complete and intact nisin gene cluster into the Bacillus subtilis 168 chromosome. Furthermore, we demonstrate that the nisin genes are transcriptionally active. These results should greatly facilitate the studies of the genes and proteins involved in nisin expression, as well as provide a standard system for the manipulation and expression of genes involved in other members of the lantibiotic family of antimicrobial peptides. PMID:17143619

  7. The carB gene encoding the large subunit of carbamoylphosphate synthetase from Lactococcus lactis is transcribed monocistronically

    DEFF Research Database (Denmark)

    Martinussen, Jan; Hammer, Karin

    1998-01-01

    The biosynthesis of carbamoylphosphate is catalysed by the heterodimeric enzyme carbamoylphosphate synthetase (CPSase). The genes encoding the two subunits in procaryotes are normally transcribed as an operon, whereas in Lactococcus lactis, the gene encoding the large subunit (carB) is shown to be...... an isolated transcriptional unit. Carbamoylphosphate is a precursor in the biosynthesis of both pyrimidine nucleotides and arginine. By mutant analysis L. lactis is shown to possess only one carB gene; the same gene product is thus required for both biosynthetic pathways. Furthermore, arginine may...... satisfy the requirement for carbamoylphosphate in pyrimidine biosynthesis through degradation by the arginine deiminase pathway. The expression of the carB gene is subject to regulation at the level of transcription by pyrimidines most probably by an attenuator mechanism. Upstream of the carB gene, an...

  8. The putrescine biosynthesis pathway in Lactococcus lactis is transcriptionally regulated by carbon catabolic repression, mediated by CcpA.

    Science.gov (United States)

    Linares, Daniel M; del Río, Beatriz; Ladero, Victor; Redruello, Begoña; Martín, María Cruz; Fernández, María; Alvarez, Miguel A

    2013-07-01

    Lactococcus lactis is the lactic acid bacterium most widely used by the dairy industry as a starter for the manufacture of fermented products such as cheese and buttermilk. However, some strains produce putrescine from agmatine via the agmatine deiminase (AGDI) pathway. The proteins involved in this pathway, including those necessary for agmatine uptake and conversion into putrescine, are encoded by the aguB, aguD, aguA and aguC genes, which together form an operon. This paper reports the mechanism of regulation of putrescine biosynthesis in L. lactis. It is shown that the aguBDAC operon, which contains a cre site at the promoter of aguB (the first gene of the operon), is transcriptionally regulated by carbon catabolic repression (CCR) mediated by the catabolite control protein CcpA. PMID:23688550

  9. Mdt(A), a New Efflux Protein Conferring Multiple Antibiotic Resistance in Lactococcus lactis and Escherichia coli

    Science.gov (United States)

    Perreten, Vincent; Schwarz, Franziska V.; Teuber, Michael; Levy, Stuart B.

    2001-01-01

    The mdt(A) gene, previously designated mef214, from Lactococcus lactis subsp. lactis plasmid pK214 encodes a protein [Mdt(A) (multiple drug transporter)] with 12 putative transmembrane segments (TMS) that contain typical motifs conserved among the efflux proteins of the major facilitator superfamily. However, it also has two C-motifs (conserved in the fifth TMS of the antiporters) and a putative ATP-binding site. Expression of the cloned mdt(A) gene decreased susceptibility to macrolides, lincosamides, streptogramins, and tetracyclines in L. lactis and Escherichia coli, but not in Enterococcus faecalis or in Staphylococcus aureus. Glucose-dependent efflux of erythromycin and tetracycline was demonstrated in L. lactis and in E. coli. PMID:11257023

  10. Multi-stress resistance in Lactococcus lactis is actually escape from purine-induced stress sensitivity

    DEFF Research Database (Denmark)

    Ryssel, Mia; Hviid, Anne-Mette Meisner; Dawish, Mohamed S.;

    2014-01-01

    to the acid-stress medium increased the stress sensitivity of L. lactis MG1363. It is also shown that high intracellular guanine nucleotide pools confer increased sensitivity to high temperatures, thus showing that it is indeed a multi-stress phenotype. Our analysis suggests that an increased level......Multi-stress resistance is a widely documented and fascinating phenotype of lactococci where single mutations, preferentially in genes involved in nucleotide metabolism and phosphate uptake, result in elevated tolerance to multiple stresses simultaneously. In this report, we have analysed the...... metabolic basis behind this multi-stress-resistance phenotype in Lactococcus lactis subsp. cremoris MG1363 using acid stress as a model of multi-stress resistance. Surprisingly, we found that L. lactis MG1363 is fully resistant to pH 3.0 in the chemically defined SA medium, contrary to its sensitivity in...

  11. Effect of Lactococcus lactis CLFP 100 and Leuconostoc mesenteroides CLFP 196 on Aeromonas salmonicida Infection in brown trout (Salmo trutta).

    Science.gov (United States)

    Balcázar, José Luis; Vendrell, Daniel; de Blas, Ignacio; Ruiz-Zarzuela, Imanol; Múzquiz, José Luis

    2009-01-01

    Aeromonas salmonicida is the etiological agent of furunculosis in salmonid fish. This pathogen is important from an epizootic perspective because fish surviving an outbreak can remain lifelong asymptomatic carriers, serving as reservoirs of infection. As a result, the early detection and the control of infection are essential to prevent the spread of new furunculosis outbreaks. We have thus analyzed the effect of probiotic administration on the incidence of A. salmonicida in brown trout (Salmo trutta), that were subjected to temperature stress. Treatment with probiotic strains (Lactococcus lactis CLFP 100 and Leuconostoc mesenteroides CLFP 196) resulted in a higher survival rate after challenge, activation of phagocytic cells in the head kidney, and a lower rate of pathogen proliferation in the intestine as determined by real-time PCR. PMID:19556745

  12. Neutropenia crónica e infección por el virus de la inmunodeficiencia humana

    Directory of Open Access Journals (Sweden)

    Ronald A Noguera-Valverde

    2008-09-01

    Full Text Available Se presenta el caso de un paciente masculino con neutropenia crónica e infección por el virus de inmunodeficiencia humana, con una revisión de los posibles mecanismos patogénicos. Las alteraciones hematológicas como anemia, trombocitopenia y leucopenia se presentan asociadas con frecuencia a la infección aguda por el virus de inmunodeficiencia humana. Al establecer la terapia antirretroviral y disminuir la actividad del virus, estas alteraciones tienden a mejorar. Sin embargo, algunos fármacos antirretrovirales, como la zidovudina, poseen toxicidad medular y pueden producir o empeorar las alteraciones hematológicas en estos pacientes, lo cual lleva a cambios en los esquemas de tratamiento. Los citotóxicos y antimetabolitos empleados en el tratamiento de neoplasias asociadas tienen conocida actividad depresora sobre la médula ósea. Algunos antimicrobianos utilizados en la profilaxis de infecciones poseen también toxicidad hematológica conocida, como el trimetoprim-sulfametoxazol, por lo que deben ser utilizados con precaución en pacientes con infección por el virus de inmunodeficiencia humana. Por otro lado, se plantean mecanismos alternativos que causan neutropenia en estos pacientes, como la formación de anticuerpos antineutrófilos, daño primario del progenitor granulocítico, por desbalance en la producción de neutrófilos, por anticuerpos contra la glicoproteína gp120 de la cápside viral del VIH, y deficiencias vitamínicas. En el caso del paciente neutropénico febril, en quien se sospecha infección bacteriana grave, se pueden utilizar los factores estimulantes de las colonias de granulocitos para aumentar los conteos absolutos de neutrófilos y mejorar la recuperación clínica.

  13. Evaluación de la eficacia de dos prescripciones de cepillos dentales en la remoción de placa bacteriana en pacientes ortodóncicos Evaluation of two toothbrushes prescriptions on the removal of plaque in patients with fixed orthodontic appliances

    Directory of Open Access Journals (Sweden)

    D Zúñiga García

    2012-12-01

    Full Text Available La aparatología empleada en los tratamientos de ortodoncia, favorece la retención de placa bacteriana y dificulta su eliminación por parte del paciente, aumentando el riesgo de desarrollar caries, manchas blancas y enfermedad periodontal. Diferentes cepillos dentales se han desarrollado para facilitar la mantención de una adecuada higiene oral en estos pacientes, sin embargo, se ha observado que los cepillos comunmente indicados (cepillo ortodóncico mas cepillo unipenacho, no siempre son bien manejados y difícilmente se usan ambos. El cepillo Cross Action Pro-Salud®, se ha presentado como una alternativa simple de usar y de transportar, por ser un único cepillo que combina las caracteristicas de los dos cepillos comunmente recomendados. Para evaluar la efectividad de este cepillo, en comparación con la prescripción convencional, se evaluaron 2 grupos, de 23 pacientes cada uno, portadores de aparatología fija. Un grupo utilizó la prescripción habitual y un segundo grupo utilizó cepillo Cross Action Pro-Salud®, por un período de 45 días. Los indices de higiene de O`leary, de placa en brackets y gingival modificado fueron registrados al inicio y 45 días después del uso diario de los cepillos antes mencionados. Se utilizó test-t para comparar los resultados obtenidos y se determinó que se produjo una disminución significativa en los tres indices de higiene, no existiendo diferencias en los resultados obtenidos entre ambas prescripciones. El cepillo Oral-B Cross-Action Pro-Salud® es una alternativa recomendable, ya que permite eliminar efectivamente la placa bacteriana en pacientes ortodónticos, y al ser un único cepillo, facilita y acorta el tiempo de cepillado.Orthodontic fixed appliances include elements that allow the accumulation of bacterial plaque, making tooth brushing more difficult and increasing the risk of developing caries, white spot lesions, and periodontal disease. Several toothbrushes designs have been

  14. Efecto de la adición de materia orgánica sobre la dinámica poblacional bacteriana del suelo en cultivos de papa y maíz Effect of addition of organic matter on bacterial population dynamics of soil in potato and corn crops

    Directory of Open Access Journals (Sweden)

    David García Ventocilla

    2012-02-01

    Full Text Available Se determinó el efecto de la fertilización orgánica sobre las poblaciones bacterianas del suelo en cultivos de papa y maíz durante la campaña agrícola 2008-2009 en terrenos de cuatro localidades del Valle del Mantaro: INIA Santa Ana (Huancayo, en la EEA El Mantaro (Jauja, Vista Alegre y Huayao (ambos en Chupaca. En estos lugares se instalaron parcelas experimentales de papa (Solanum tuberosum L. Var. Canchan y maíz (Zea maíz L. Var. Cusco mejorado bajo abonamiento orgánico (vacuno, ovino, cuy, fertilización química y sin fertilización alguna (testigo. Para dicho efecto se empleó las técnicas de la Electroforesis en Gel de Gradiente Desnaturalizante (DGGE con amplificación de la región 968 – 1401 del rDNA 16S. Los resultados obtenidos muestran que la variabilidad de las poblaciones bacterianas en los suelos está afectado directamente por el tipo de cultivo mas no por el tipo de fertilización ya que el efecto de este último resulta variable para cada zona experimental y cultivo encontrándose solo en la zona experimental de Chupaca - Maíz una segregación de los tratamientos con fertilización orgánica de los tratamientos químicos. También se ha encontrado que la variación de las comunidades microbianas no sufre variaciones significativas en los suelos con cultivos de maíz obteniéndose coeficientes de similaridad para todos los tratamientos por encima del 80% mientras que para los tratamientos en los cultivos de papa dicho coeficiente fue de tan solo del 60%.The effect of organic fertilization on soil bacterial populations in potato and corn crops during the crop season 2008-2009 at four sites in the Mantaro Valley locations: INIA Santa Ana (Huancayo, the EEA El Mantaro (Jauja, Vista Alegre and Huayao (both in Chupaca. In these places were set up experimental plots of potato (Solanum tuberosum L. var. Canchan and corn (Zea maize L. Var. Cusco enhanced under organic manure (cattle, sheep, guinea pig, chemical fertilizer

  15. Escova dental e dedeira na remoção da placa bacteriana dental em cães The dental brush and thumb-stall in the removal of the dental plaque in dogs

    Directory of Open Access Journals (Sweden)

    Tânia Berbert Ferreira Lima

    2004-02-01

    Full Text Available A placa bacteriana é fator primário na formação de gengivite, cálculo dentário, halitose e doença periodontal. Objetivou-se avaliar a quantidade de placa bacteriana dental removida pela escova dental e dedeira. Foram utilizados 60 cães machos e fêmeas de diferentes raças, idade e peso, divididos em dois grupos. O índice Logan & Boyce foi utilizados para quantificar a placa bacteriana antes e após a escovação. Observou-se diferença estatística (p0,05 entre a utilização da escova dental e a dedeira.The dental plaque is the primary factor for gingivitis formation, dental calculus, oral malodor and periodontal disease. To evaluate the amount of dental plaque removed by the dental brush and thumb-stall, 60 male and female dogs of different races, age and weight were divided in two groups and studies. The index of Logan & Boyce was used to quantify the dental plaque before and after the toothbrush. Statistical difference was observed (p 0.05 between the use of the dental brush and the thumb-stall.

  16. Poblaciones bacterianas utilizadoras de hidrógeno presentes en el tracto gastrointestinal del avestruz (Struthio camelus Var. Domesticus

    Directory of Open Access Journals (Sweden)

    J. M. Miramontes-Carrillo

    2008-01-01

    Full Text Available Tres grupos de bacterias compiten por hidrógeno en diferentes ecosistemas anaerobios, y junto con CO 2 , producen metano, sulfuro dehidrógeno y acetato. Estas reacciones representan ganancia de energía al animal. Motivo por el cual, el presente trabajo pretende evaluar la presencia y tamaño de poblaciones de bacterias utilizadoras de hidrógeno en el tracto gastrointestinaldel avestruz. Para ello se utilizaron medios AC11 para acetogénicas, Potgate para sulfato-reductoras y Fosfato buffer para metanogénicas. Las poblaciones se determinaron por el método del número más probable (NMP. El diseño fue completamente al azar, con arreglo factorial, A = porciones del tracto digestivo y B = microorganismo. El NMP, de acetogénicas y metanogénicas fue cero. Las bacterias sulfato-reductoras están presentes en todo el tracto gastrointestinal. Las poblaciones fueron 544.00; 532.00; 157.20; 155.32 y 76.48 x 10/ 6 para el intestino grueso, ciegos, intestino delgado, proventrículo y ventrículo, respectivamente. Los resultados confirman presencia y predominio de las sulfato-reductoras en todas las porciones del tracto gastrointestinal del Struthio camelus. La producción de AGV en el tracto gastrointestinal del avestruz, es producto del metabolismo y reutilización del hidrógeno por bacterias sulfato-reductoras.

  17. Enfermedad respiratoria grave en terapia intensiva durante la pandemia por el virus de influenza A (H1N1 2009

    Directory of Open Access Journals (Sweden)

    José Aquino-Esperanza

    2010-10-01

    Full Text Available Se describen pacientes hospitalizados en una unidad de terapia intensiva por enfermedad respiratoria aguda grave con características de influenza durante los primeros meses de la pandemia por influenza A(H1N1 2009 en la Argentina. Evaluamos datos clínicos, scores de gravedad, pruebas de laboratorio, microbiología y radiología torácica al ingreso, evolución y mortalidad hospitalaria, comparando pacientes con y sin confirmación de H1N1 por test de reacción de polimerasa en cadena, transcriptasa reversa (RT-PCR. Entre junio y julio de 2009 se internaron 31 pacientes adultos con una mediana de edad de 54 años (percentilo 25-75: 33-66. Presentaron test positivo para H1N1, 17 pacientes. Tenían al menos una condición concurrente 16 pacientes. La expresión radiográfica más frecuente fue infiltrados intersticio-alveolares bilaterales en 20 casos; 5 tenían consolidación lobar unilateral. La coinfección bacteriana (aislamiento de bacterias o IgM positiva para infecciones bacterianas, se demostró en 21 pacientes. Requirieron ventilación mecánica 23 pacientes y 18 desarrollaron síndrome de distrés respiratorio agudo (SDRA. La linfopenia y elevación de creatinina-fosfoquinasa fue frecuente (83% y 65%, respectivamente. Los 6 pacientes que murieron (19% eran mayores de 75 años o tenían cáncer o inmunodepresión. El tratamiento antiviral temprano (≤ 48 horas se asoció a menor necesidad de ventilación mecánica (54% vs. 89%; p: 0.043. No hubo diferencia significativa en las variables analizadas entre el grupo H1N1 positivo y el negativo, lo que sugiere tener igual enfoque terapéutico frente a una epidemia. La infección por H1N1 determinó falla respiratoria aguda y SDRA. La mortalidad ocurrió en pacientes añosos o con co-morbilidades graves.

  18. Bacteriocinogenic Lactococcus lactis subsp. lactis DF04Mi isolated from goat milk: Application in the control of Listeria monocytogenes in fresh Minas-type goat cheese

    OpenAIRE

    Danielle N. Furtado; Todorov, Svetoslav D.; Mariza Landgraf; Destro, Maria T.; Bernadette D.G.M. Franco

    2015-01-01

    Listeria monocytogenes is a pathogen frequently found in dairy products. Its control in fresh cheeses is difficult, due to the psychrotrophic properties and salt tolerance. Bacteriocinogenic lactic acid bacteria (LAB) with proven in vitro antilisterial activity can be an innovative technological approach but their application needs to be evaluated by means of in situ tests. In this study, a novel bacteriocinogenic Lactococcus lactis strain ( Lc . lactis DF4Mi), isolated from raw goat milk, wa...

  19. Determination of the phosphorylated sugars of the Embden-Meyerhoff-Parnas Pathway in Lactococcus lactis using a fast sampling technique and solid phase extraction

    DEFF Research Database (Denmark)

    Jensen, Niels B.s.; Jokumsen, Kirsten Væver; Villadsen, John

    1999-01-01

    An experimental procedure for the determination of intracellular concentrations of the phosphorylated sugars in the lactic acid bacterium Lactococcus lactis is presented. The first step of the procedure is a rapid sampling of a small volume of the growth medium into 60% (v/v) methanol precooled t...... components. The internal standard was recovered to an extent of 85-95%. (C) 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 63: 356-362, 1999....

  20. Mucosal Delivery of Murine Interleukin-2 (IL-2) and IL-6 by Recombinant Strains of Lactococcus lactis Coexpressing Antigen and Cytokine

    OpenAIRE

    Steidler, Lothar; Robinson, K.; Chamberlain, L.; SCHOFIELD, KM; Remaut, Erik; LE PAGE, RWF; Wells, JM

    1998-01-01

    Lactococcus lactis is a nonpathogenic and noncolonizing bacterium which is being developed as a vaccine delivery vehicle for immunization by mucosal routes. To determine whether lactococci can also deliver cytokines to the immune system, we have constructed novel constitutive expression strains of L. lactis which accumulate a test antigen, tetanus toxin fragment C (TTFC), within the cytoplasmic compartment and also secrete either murine interleukin-2 (IL-2) or IL-6. When mice were immunized i...

  1. Draft Genome Sequence of Lactococcus garvieae Strain PAQ102015-99, an Outbreak Strain Isolated from a Commercial Trout Farm in the Northwestern United States.

    Science.gov (United States)

    Nelson, Michael C; Varney, Jed S; Welch, Timothy J; Graf, Joerg

    2016-01-01

    We announce the draft genome assembly of Lactococcus garvieae strain PAQ102015-99, a recently isolated strain from an outbreak of lactococcosis at a commercial trout farm in the northwestern United States. The draft genome comprises 14 contigs totaling 2,068,357 bp with an N50 of 496,618 bp and average G+C content of 38%. PMID:27492003

  2. Improvement of LysM-Mediated Surface Display of Designed Ankyrin Repeat Proteins (DARPins) in Recombinant and Nonrecombinant Strains of Lactococcus lactis and Lactobacillus Species

    OpenAIRE

    Zadravec, Petra; Štrukelj, Borut; Berlec, Aleš

    2015-01-01

    Safety and probiotic properties make lactic acid bacteria (LAB) attractive hosts for surface display of heterologous proteins. Protein display on nonrecombinant microorganisms is preferred for therapeutic and food applications due to regulatory requirements. We displayed two designed ankyrin repeat proteins (DARPins), each possessing affinity for the Fc region of human IgG, on the surface of Lactococcus lactis by fusing them to the Usp45 secretion signal and to the peptidoglycan-binding C ter...

  3. Transport of β-Casein-derived Peptides by the Oligopeptide Transport System Is a Crucial Step in the Proteolytic Pathway of Lactococcus lactis

    OpenAIRE

    Kunji, E. R. S.; Hagting, A; de Vries, C. J.; Juillard, V; Haandrikman, A J; Poolman, B; Konings, W. N.

    1995-01-01

    In the proteolytic pathway of Lactococcus lactis, milk proteins (caseins) are hydrolyzed extracellularly to oligopeptides by the proteinase (PrtP). The fate of these peptides, i.e. extracellular hydrolysis followed by amino acid uptake or transport followed by intracellular hydrolysis, has been addressed. Mutants have been constructed that lack a functional di-tripeptide transport system (DtpT) and/or oligopeptide transport system (Opp) but do express the P-1-type proteinase (specific for hyd...

  4. Degradation and debittering of a tryptic digest from beta-casein by aminopeptidase N from Lactococcus lactis subsp. cremoris Wg2.

    OpenAIRE

    Tan, P S; van Kessel, T A; van de Veerdonk, F.L.; Zuurendonk, P F; Bruins, A P; Konings, W. N.

    1993-01-01

    The mode of action of purified aminopeptidase N from Lactococcus lactis subsp. cremoris Wg2 on a complex peptide mixture of a tryptic digest from bovine beta-casein was analyzed. The oligopeptides produced in the tryptic digest before and after aminopeptidase N treatment were identified by analysis of the N- and C-terminal amino acid sequences and amino acid compositions of the isolated peptides and by on-line liquid chromatography-mass spectrometry. Incubation of purified peptides with amino...

  5. Heterotrophic bacteria abundances in Rodrigo de Freitas Lagoon (Rio de Janeiro, Brazil Abundância bacteriana heterotrófica na Lagoa Rodrigo de Freitas (Rio de Janeiro, Brasil

    Directory of Open Access Journals (Sweden)

    Alessandra M. Gonzalez

    2006-12-01

    " em ágar R2A. Outro objetivo foi avaliar a influência da lagoa na qualidade das águas de Ipanema e do Leblon. Dados físico-químicos também foram adquiridos. Amostras de água sub-superficiais foram coletadas mensalmente, de dezembro de 1999 a outubro de 2000. Na lagoa, a abundância de bactérias cultivadas variou de 6,9x10(5 a 5,0x10(7 UFC.100 mL-1. Em Ipanema e no Leblon, esse parâmetro atingiu 1,4x10(5 e 2,8x10(6 UFC.100 mL-1, respectivamente. A abundância bacteriana total variou de 2,9x10(7 a 3,2x10(7 células.mL-1 na lagoa. Em Ipanema e no Leblon, este parâmetro alcançou, respectivamente, 8,7x10(6 a 1,1x10(7 células.mL-1. Dois sub-grupos bacterianos foram observados nas estações, com predomínio de células HNA. Amostras foram posteriormente coletadas visando um maior entendimento sobre as bactérias presentes nesses ambientes. A abundância bacteriana foi analisada apenas por citometria em fluxo e os resultados oscilaram entre 8,3x10(6 e 2,5x10(7 células.mL-1 na lagoa. Na praia, esse parâmetro atingiu 6,9x10(6 células.mL-1. Dois sub-grupos bacterianos também foram observados, com predomínio de células HNA na lagoa e LNA na praia adjacente. Os resultados indicaram que a lagoa é um ecossistema eutrofizado, onde as bactérias e os parâmetros físico-químicos distribuem-se de modo uniforme. Os dados ainda indicaram que as águas poluídas da lagoa afetam a qualidade das praias de Ipanema e do Leblon.

  6. Osteomielitis por salmonella

    Directory of Open Access Journals (Sweden)

    Alicia Velázquez Pérez

    2014-08-01

    Full Text Available Se presenta el caso de una paciente femenina de color blanco y dos años de edad, con diagnóstico prenatal de sicklemia, que desde edades tempranas tiene problemas de la enfermedad. Ingresó en esta ocasión por una de las complicaciones infecciosas que ocasiona este padecimiento, una osteomielitis del húmero izquierdo, aislándose el germen en el hemocultivo realizado, una salmonella. Necesitó de tratamiento enérgico y prolongado; se obtuvo un resultado satisfactorio en la evolución de la enfermedad y se sigue sistemáticamente por consulta externa en la actualidad

  7. tirados por tractores

    Directory of Open Access Journals (Sweden)

    Ciro E. Iglesias Coronel

    2006-01-01

    Full Text Available En el presente trabajo se brinda la metodología de realización y evaluación de los principales indicadores energéticos de los agregados de transporte agrícola, formados por tractores y remolques con el uso de la tensometría como herramienta para la toma de los datos experimentales de campo.

  8. Application of a bacterial extracellular polymeric substance in heavy metal adsorption in a co-contaminated aqueous system Aplicação de uma substância polimerica extracelular bacteriana na absorção de metais pesados em um sistema aquoso co-contaminado

    Directory of Open Access Journals (Sweden)

    Paula Salles de Oliveira Martins

    2008-12-01

    Full Text Available The application of a bacterial extracellular polymeric substance (EPS in the bioremediation of heavy metals (Cd, Zn and Cu by a microbial consortium in a hydrocarbon co-contaminated aqueous system was studied. At the low concentrations used in this work (1.00 ppm of each metal, it was not observed an inhibitory effect on the cellular growing. In the other hand, the application of the EPS lead to a lower concentration of the free heavy metals in solution, once a great part of them is adsorbed in the polymeric matrix (87.12% of Cd; 19.82% of Zn; and 37.64% of Cu, when compared to what is adsorbed or internalized by biomass (5.35% of Cd; 47.35% of Zn; and 24.93% of Cu. It was noted an increase of 24% in the consumption of ethylbenzene, among the gasoline components that were quantified, in the small interval of time evaluated (30 hours. Our results suggest that, if the experiments were conducted in a larger interval of time, it would possibly be noted a higher effect in the degradation of gasoline compounds. Still, considering the low concentrations that were evaluated, it is possible that a real system could be bioremediated by natural attenuation process, demonstrated by the low effect of those levels of contaminants and co-contaminants over the naturally present microbial consortium.A aplicação de uma substância polimérica extracelular (EPS bacteriana na biorremediação de metais pesados (Cd, Zn e Cu por um consórcio microbiano em um sistema aquoso co-contaminado com hidrocarbonetos foi estudada. Nas baixas concentrações usadas neste trabalho (1,00 ppm de cada metal, não foi observado um efeito inibitório no crescimento celular. Por outro lado, a aplicação da EPS bacteriana levou a uma menor concentração de metais livres em solução, uma vez que grande parte destes fica adsorvido na matriz polimérica (87,12% de Cd; 19,82% de Zn; e 37,64% de Cu quando comparado ao que é adsorvido ou interiorizado pela biomassa (5,35% de Cd; 47

  9. The clinicopathological and epidemiological aspects of bacterial endocarditis in dogs: 28 cases (2003-2008 / Aspectos clínico-patológicos e epidemiológicos da endocardite bacteriana em cães: 28 casos (2003-2008

    Directory of Open Access Journals (Sweden)

    Patrícia Mendes Pereira

    2010-04-01

    Full Text Available The aim of this study was to review the clinicopathological aspects and report the data obtained from bacterial endocarditis cases in dogs. A restropective study was carried out based on files of the Veterinary Hospital and the Animal Pathology Laboratory of Universidade Estadual de Londrina (UEL from January 2003 to may 2008. During this period, 28 dogs presented bacterial endocarditis, 50% males and 50% females. Medium to large dogs were the most affected and adult animals were prevalent, with the following clinical signs: apathy, hiporexy, hyperthermia, splenomegaly, dyspnea, dehydration, dysuria and cardiac murmur, Necropsy revealed the mitral valve as the most affected cardiac structure and among the organs, the kidneys were the most affected.O presente trabalho objetivou revisar os aspectos clínico-patológicos e relatar os dados obtidos sobre a endocardite bacteriana em cães, por meio de estudo retrospectivo realizado nos arquivos do Hospital Veterinário e Laboratório de Patologia Animal da Universidade Estadual de Londrina (UEL, durante o período de janeiro de 2003 a maio de 2008. No transcorrer do período considerado, 28 cães apresentaram a doença, sendo 50% machos e 50% fêmeas. Animais de médio a grande porte foram os mais acometidos e os adultos obtiveram prevalência. Os principais sinais clínicos apresentados foram apatia, hiporexia, hipertermia, esplenomegalia, dispnéia, desidratação, disúria e sopro cardíaco. À necropsia, observou-se que a válvula mitral foi a estrutura cardíaca mais afetada, sendo o rim o órgão mais frequentemente acometido.

  10. Hiperinfestação por Strongyloides Stercoralis - doi:10.5020/18061230.2006.p118

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    Tânia Maria Cavalcante Maia

    2012-01-01

    Full Text Available A estrongiloidíase é uma helmintíase predominantemente intestinal causada pelo Strongyloides stercoralis, sendo o homem seu hospedeiro definitivo, além da principal fonte de infecção. Este trabalho objetivou relatar um caso de identificação laboratorial de larva de Strongyloides stercolaris no líquido ascítico de um paciente do sexo masculino, 49 anos, agricultor, etilista há mais de 30 anos, encaminhado ao Hospital Universitário Walter Cantídio, por estar evoluindo com episódios de icterícia associada a aumento do volume abdominal diagnosticado como ascite, vômito, diarréia pastosa e diminuição do volume urinário. No internamento, foi iniciada medicação profilática para Peritonite Bacteriana Espontânea (PBE, bem como reposição de vitaminas, hidratação venosa e diuréticos, sem sucesso terapêutico. A partir de achados no líquido ascítico de presença de larvas de Strongyloides stercolaris em atividade, o quadro foi interpretado como estrongiloidíase disseminada em paciente imunocomprometido por hepatopatia avançada e alcoólica. O paciente evoluiu com quadro de choque caracterizado por hipotensão e hipotermia, tendo chegado a fazer uso de drogas vasoativas de forma não responsiva. O quadro evoluiu para óbito. Os autores chamam a atenção para a importância do diagnóstico e tratamento precoce da estrongiloidíase, principalmente em pacientes imunodeprimidos.

  11. Peritonite bacteriana espontânea na cirrose hepática: prevalência, fatores preditivos e prognóstico Spontaneous bacterial peritonitis in hepatic cirrhosis: prevalence, predictive factors and prognosis

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    F.A.F. Figueiredo

    1999-04-01

    Full Text Available OBJETIVO: Investigar prevalência, fatores preditivos e prognóstico dos episódios de Peritonite Bacteriana Espontânea (PBE na cirrose hepática. METODOLOGIA: Estudamos, prospectivamente, 143 pacientes com cirrose hepática, ambulatoriais ou internados, que foram atendidos nos Serviços de Clínica Médica do HUCFF e de Gastroenterologia do HUPE no período de janeiro/95 a janeiro/96. Estes pacientes foram submetidos a questionário, exame físico, colheita de sangue e paracentese abdominal com colheita de líquido ascítico (LA e, então, acompanhados por um período médio de 4 meses, onde a taxa de mortalidade foi determinada. RESULTADOS: A prevalência de PBE foi cerca de 20%, sendo 24% PBE cultura positiva, 66% Ascite Neutrofílica cultura negativa e 10% Bacterioascite. Na análise univariada, alcançaram significância estatística (p=0.05 como fatores preditivos do episódio de PBE: HGI na semana anterior; passado de encefalopatia hepática; classificação de Child; dosagens séricas de proteínas, albumina, C3, C4 e uréia; dosagens no LA de C3 e C4. Após serem introduzidas na análise multivariada, apenas HGI na semana anterior, albumina sérica e C4 do LA foram independentemente correlacionadas ao episódio de PBE (p=0.05. A mortalidade hospitalar e durante o acompanhento foi de 33,3% e 53,8% para o grupo com PBE; 8,5% e 31,9% para o grupo sem PBE; respectivamente (p=0.01 e p=0.04. A probabilidade cumulativa de sobrevida foi significativamente menor no grupo com PBE. CONCLUSÕES: A PBE é uma complicação freqüente, depende, principalmente, da gravidade da doença hepática e é um marcador de prognóstico desfavorável nos pacientes com cirrose hepática.BACKGROUND: Spontaneous Bacterial Peritonitis (SBP is a common and potentially fatal complication of cirrhosis. Multiple variants of this infection have been described during the past decade. Few studies have investigated SBP in Brazil. MATERIAL AND METHOD: In order to

  12. Diversidade e potencial biotecnológico da comunidade bacteriana endofítica de sementes de soja Diversity and biotechnological potential of endophytic bacterial community of soybean seeds

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    Laura de Castro Assumpção

    2009-05-01

    Full Text Available O objetivo deste trabalho foi isolar, caracterizar e identificar a comunidade bacteriana endofítica de sementes de soja e avaliar o seu potencial biotecnológico. Foram utilizadas sementes de 12 cultivares de soja. Os isolados bacterianos endofíticos obtidos foram avaliados in vitro quanto ao antagonismo a fungos fitopatogênicos, síntese de ácido indolacético (AIA e solubilização de fosfato. A caracterização foi realizada com técnicas de isolamento, análise de restrição do DNA ribossomal amplificado (ARDRA e sequenciamento parcial do gene 16S rDNA. Os isolados com maior potencial biotecnológico foram inoculados em sementes de soja, para se avaliar a capacidade de promoção de crescimento de plantas. Foi possível identificar 12 ribótipos por meio da ARDRA, que foram classificados como: Acinetobacter, Bacillus, Brevibacterium, Chryseobacterium, Citrobacter, Curtobacterium, Enterobacter, Methylobacterium, Microbacterium, Micromonospora, Pantoea, Paenibacillus, Pseudomonas, Ochrobactrum, Streptomyces e Tsukamurella. Quanto ao potencial biotecnológico da comunidade, 18% dos isolados controlaram o crescimento de fungos fitopatogênicos, 100% produziram AIA, e 39% solubilizaram fosfato. O isolado 67A(57 de Enterobacter sp. aumentou significativamente a massa de matéria seca da raiz. A inoculação de isolados com elevado potencial biotecnológico em avaliações in vitro não promoveu o crescimento de plantas de soja na maioria dos casos.The objectives of this work were to isolate, characterize and identify the endophytic bacterial community of soybean seeds, and to test the biotechnological potential of this community. Seeds from 12 soybean cultivars were used. The endophytic bacterial isolates were evaluated for in vitro antagonism against phytopathogenic fungi, synthesis of indoleacetic acid (IAA, and capacity to solubilize phosphate. Isolation techniques, amplified ribosomal DNA restriction analysis (ARDRA grouping, and

  13. Avaliação da contaminação bacteriana em desinfetantes de uso domiciliar

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    Miyagi Fumie

    2000-01-01

    Full Text Available OBJETIVO: Avaliar desinfetantes de uso domiciliar, identificando a presença de bactérias contaminantes, e conhecer o nível de tolerância dessas bactérias ao cloreto de benzalcônio. MÉTODOS: Foram adquiridas aleatoriamente no comércio da região metropolitana de São Paulo, SP, Brasil, 52 amostras de desinfetantes de uso domiciliar para análise quanto à presença de bactérias contaminantes. O nível de tolerância dessas bactérias ao cloreto de benzalcônio foi determinado pelo método da macrodiluição em caldo. RESULTADOS: De 52 amostras, 16 (30,77% estavam contaminadas por bactérias Gram negativas, com contagens variando entre 10(4 e 10(6 UFC/ml. Esses contaminantes foram identificados como Alcaligenes xylosoxidans, Burkholderia cepacia e Serratia marcescens. As Concentrações Inibitórias Mínimas (CIM: mg/ml do cloreto de benzalcônio para S. marcescens, A. xylosoxidans e B. cepacia foram: 2,48, 1,23 e 0,30, respectivamente. CONCLUSÕES Os desinfetantes de uso domiciliar à base de compostos de amônio quaternário são passíveis de contaminação por bactérias. As CIM do cloreto de benzalcônio para as bactérias contaminantes estavam abaixo das concentrações do princípio ativo presente nos desinfetantes, indicando que a tolerância ao biocida não é estável, podendo ser perdida com o cultivo das bactérias em meios de cultura sem o biocida.

  14. Avaliação da contaminação bacteriana em desinfetantes de uso domiciliar

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    Fumie Miyagi

    2000-10-01

    Full Text Available OBJETIVO: Avaliar desinfetantes de uso domiciliar, identificando a presença de bactérias contaminantes, e conhecer o nível de tolerância dessas bactérias ao cloreto de benzalcônio. MÉTODOS: Foram adquiridas aleatoriamente no comércio da região metropolitana de São Paulo, SP, Brasil, 52 amostras de desinfetantes de uso domiciliar para análise quanto à presença de bactérias contaminantes. O nível de tolerância dessas bactérias ao cloreto de benzalcônio foi determinado pelo método da macrodiluição em caldo. RESULTADOS: De 52 amostras, 16 (30,77% estavam contaminadas por bactérias Gram negativas, com contagens variando entre 10(4 e 10(6 UFC/ml. Esses contaminantes foram identificados como Alcaligenes xylosoxidans, Burkholderia cepacia e Serratia marcescens. As Concentrações Inibitórias Mínimas (CIM: mg/ml do cloreto de benzalcônio para S. marcescens, A. xylosoxidans e B. cepacia foram: 2,48, 1,23 e 0,30, respectivamente. CONCLUSÕES Os desinfetantes de uso domiciliar à base de compostos de amônio quaternário são passíveis de contaminação por bactérias. As CIM do cloreto de benzalcônio para as bactérias contaminantes estavam abaixo das concentrações do princípio ativo presente nos desinfetantes, indicando que a tolerância ao biocida não é estável, podendo ser perdida com o cultivo das bactérias em meios de cultura sem o biocida.

  15. Miotoxicidade por organofosforados

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    Cavaliere Maria J.

    1996-01-01

    Full Text Available Os organofosforados são um grupo de compostos químicos amplamente utilizados em agropecuária como inseticidas, ocasionando intoxicações acidentais em animais e humanos, e mesmo sendo utilizados em tentativas de suicídio. A toxicidade desses produtos decorre sobretudo de insuficiência cárdio-respiratória por compromentimento do sistema nervoso autônomo. Sabe-se que alguns destes compostos induzem em animais de experimentação e em humanos, uma miopatia caracterizada por degeneração de células musculares, comprometendo sobretudo a musculatura respiratória. Baseado no fato de que este comprometimento contribui para a piora da função respiratória, propõe-se um protocolo de avaliação rotineira de miotoxicidade por compostos organofosforados, através de uma bateria mínima e suficiente de colorações e reações histoquímicas para quantificação da necrose muscular. Utilizaram-se como modelo experimental, grupos de ratos albinos (Wistar intoxicados com o organofosforado paraoxon, com e sem antídotos (atropina ou pralidoxima. Verificou-se nos grupos tratados com paraoxon e paraoxon mais atropina, necrose de fibras musculares no diafragma, que atingia em determinadas áreas até 15% das fibras. No grupo tratado com paraoxon mais pralidoxima, a necrose foi mínima, evidenciando o papel mioprotetor deste último antídoto.

  16. Nucleotomia por laser

    OpenAIRE

    Cabral, oão José Lobato Guimarães Ferreira

    2009-01-01

    A hérnia de disco intervertebral com compressão nervosa que não cede ao tratamento médico, tradicionalmente, é indicação para tratamento cirúrgico. No entanto, técnicas minimamente invasivas e os procedimentos percutâneos têm sido usados e desenvolvidos nas últimas duas décadas, tanto como procedimentos alternativos, bem como possibilidades terapêuticas que antecedem o tratamento cirúrgico convencional, podendo adiar o mesmo por tempo indeterminado. De entre estas opções técnicas, surge a Nuc...

  17. Por mil devaluados pesos

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    Annie Rodríguez Collázos

    2013-03-01

    Full Text Available El estudio de lo popular y lo urbano hasta ahora se ha centrado en el comportamiento y en algunas relaciones de los habitantes con su entorno. “Por mil devaluados pesos. Publicidad popular y urbana”, pretende explorar las formas de publicidad, dispersas en diferentes espacios populares y urbanos en Bogotá, identificando esquemas y formas características de sus propios códigos comunicativos; se centran en un objeto de estudio consistente en las estrategias publicitarias y los códigos comunicativos en los mensajes publicitarios populares en las subculturas de San Victorino, 7 de Agosto y Sanandresito de San José.

  18. Nisin production of Lactococcus lactis N8 with hemin-stimulated cell respiration in fed-batch fermentation system.

    Science.gov (United States)

    Kördikanlıoğlu, Burcu; Şimşek, Ömer; Saris, Per E J

    2015-01-01

    In this study, nisin production of Lactococcus lactis N8 was optimized by independent variables of glucose, hemin and oxygen concentrations in fed-batch fermentation in which respiration of cells was stimulated with hemin. Response surface model was able to explain the changes of the nisin production of L. lactis N8 in fed-batch fermentation system with high fidelity (R(2) 98%) and insignificant lack of fit. Accordingly, the equation developed indicated the optimum parameters for glucose, hemin, and dissolved oxygen were 8 g L(-1) h(-1) , 3 μg mL(-1) and 40%, respectively. While 1711 IU mL(-1) nisin was produced by L. lactis N8 in control fed-batch fermentation, 5410 IU mL(-1) nisin production was achieved within the relevant optimum parameters where the respiration of cell was stimulated with hemin. Accordingly, nisin production was enhanced 3.1 fold in fed-batch fermentation using hemin. In conclusion the nisin production of L. lactis N8 was enhanced extensively as a result of increasing the biomass by stimulating the cell respiration with adding the hemin in the fed-batch fermentation. PMID:25826783

  19. Structure-guided engineering of Lactococcus lactis alcohol dehydrogenase LlAdhA for improved conversion of isobutyraldehyde to isobutanol

    KAUST Repository

    Liu, Xiang

    2013-03-01

    We have determined the X-ray crystal structures of the NADH-dependent alcohol dehydrogenase LlAdhA from Lactococcus lactis and its laboratory-evolved variant LlAdhA(RE1) at 1.9Å and 2.5Å resolution, respectively. LlAdhA(RE1), which contains three amino acid mutations (Y50F, I212T, and L264V), was engineered to increase the microbial production of isobutanol (2-methylpropan-1-ol) from isobutyraldehyde (2-methylpropanal). Structural comparison of LlAdhA and LlAdhA(RE1) indicates that the enhanced activity on isobutyraldehyde stems from increases in the protein\\'s active site size, hydrophobicity, and substrate access. Further structure-guided mutagenesis generated a quadruple mutant (Y50F/N110S/I212T/L264V), whose KM for isobutyraldehyde is ∼17-fold lower and catalytic efficiency (kcat/KM) is ∼160-fold higher than wild-type LlAdhA. Combining detailed structural information and directed evolution, we have achieved significant improvements in non-native alcohol dehydrogenase activity that will facilitate the production of next-generation fuels such as isobutanol from renewable resources.

  20. Expression of Helicobacter pylori hspA Gene in Lactococcus lactis NICE System and Experimental Study on Its Immunoreactivity

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    Xiao-Juan Zhang

    2015-01-01

    Full Text Available Aim. The aim of this study was to develop an oral Lactococcus lactis (L. lactis vaccine against Helicobacter pylori (H. pylori. Methods. After L. lactis NZ3900/pNZ8110-hspA was constructed, growth curves were plotted to study whether the growth of recombinant L. lactis was affected after hspA was cloned into L. lactis and whether the growth of empty bacteria, empty plasmid bacteria, and recombinant L. lactis was affected by different concentrations of Nisin; SDS-PAGE and Western blot were adopted, respectively, to detect the HspA expressed by recombinant L. lactis and its immunoreactivity. Results. There was no effect observed from the growth curve after exogenous gene hspA was cloned into L. lactis NZ3900; different concentrations of Nisin did not affect the growth of NZ3900 and NZ3900/pNZ8110, while different concentrations of Nisin inhibited the growth of NZ3900/pNZ8110-hspA except 10 ng/mL Nisin. No HspA strip was observed from SDS-PAGE. Western blot analysis showed that HspA expressed by recombinant bacteria had favorable immunoreactivity. Conclusion. The growth of recombinant L. lactis was suppressed even though a small amount of HspA had been induced to express. Therefore recombinant L. lactis only express HspA which was not suitable to be oral vaccine against Helicobacter pylori.

  1. Interaction of Saccharomyces cerevisiae and Lactococcus lactis in the fermentation and quality of artisanal cachaça

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    Fernanda Paula Carvalho

    2014-11-01

    Full Text Available Lactococcus lactis and Saccharomyces cerevisiae in co-culture were evaluated during sugar cane fermentantion for cachaça production. The inocula containing L. lactis UFLA CA 312 and S. cerevisiae UFLA CA 11 were used in the population of approximately 105 CFU mL-1 and 108 CFU mL-1,  respectively. The sugar cane medium plus 1% of yeast extract (SCM was efficient for growth of L. lactis UFLA CA 312 and S. cerevisiae UFLA CA 11 (letter b -Tukey test. In flasks and vats fermentation the growth of UFLA CA 11 was not negatively influenced by L. lactis UFLA CA 312. However, after 19 h of fermentation, bacterial population showed a slight decrease. Considering parameters higher alcohols and aldehydes, cachaça produced by pure culture of S. cerevisiae was similar to cachaça produced by mixed culture. Cachaça produced by mixed culture showed high values of volatile acidity (letter b -Scott-Knott test being characterized by this parameters in the principal component analysis. High percentage of acceptance (81.10% for the attribute aroma was observed in samples from cachaça produced by mixed culture.

  2. Identification and characterization of tetracycline resistance in Lactococcus lactis isolated from Polish raw milk and fermented artisanal products.

    Science.gov (United States)

    Zycka-Krzesinska, Joanna; Boguslawska, Joanna; Aleksandrzak-Piekarczyk, Tamara; Jopek, Jakub; Bardowski, Jacek K

    2015-10-15

    To assess the occurrence of antibiotic-resistant Lactic Acid Bacteria (LAB) in Polish raw milk and fermented artisanal products, a collection comprising 500 isolates from these products was screened. Among these isolates, six strains (IBB28, IBB160, IBB161, IBB224, IBB477 and IBB487) resistant to tetracycline were identified. The strains showing atypical tetracycline resistance were classified as Lactococcus lactis: three of them were identified as L. lactis subsp. cremoris (IBB224, IBB477 and IBB487) and the other three (IBB28, IBB160, IBB161) were identified as L. lactis subsp. lactis. The mechanism involving Ribosomal Protection Proteins (RPP) was identified as responsible for tetracycline resistance. Three of the tested strains (IBB28, IBB160 and IBB224) had genes encoding the TetS protein, whereas the remaining three (IBB161, IBB477 and IBB487) expressed TetM. The results also demonstrated that the genes encoding these proteins were located on genetic mobile elements. The tet(S) gene was found to be located on plasmids, whereas tet(M) was found within the Tn916 transposon. PMID:26204235

  3. Hsp65-producing Lactococcus lactis prevents experimental autoimmune encephalomyelitis in mice by inducing CD4+LAP+ regulatory T cells.

    Science.gov (United States)

    Rezende, Rafael M; Oliveira, Rafael P; Medeiros, Samara R; Gomes-Santos, Ana C; Alves, Andrea C; Loli, Flávia G; Guimarães, Mauro A F; Amaral, Sylvia S; da Cunha, André P; Weiner, Howard L; Azevedo, Vasco; Miyoshi, Anderson; Faria, Ana M C

    2013-02-01

    Heat shock proteins (Hsps) participate in the cellular response to stress and they are hiperexpressed in inflammatory conditions. They are also known to play a major role in immune modulation, controlling, for instance, autoimmune responses. In this study, we showed that oral administration of a recombinant Lactococcus lactis strain that produces and releases LPS-free Hsp65 prevented the development of experimental autoimmune encephalomyelitis (EAE) in C57BL/6 mice. This was confirmed by the reduced inflammatory cell infiltrate and absence of injury signs in the spinal cord. The effect was associated with reduced IL-17 and increased IL-10 production in mesenteric lymph node and spleen cell cultures. Hsp65-producing-L. lactis-fed mice had a remarkable increase in the number of natural and inducible CD4+Foxp3+ regulatory T (Treg) cells and CD4+LAP+ (Latency-associated peptide) Tregs - which express the membrane-bound TGF-β - in spleen, inguinal and mesenteric lymph nodes as well as in spinal cord. Moreover, many Tregs co-expressed Foxp3 and LAP. In vivo depletion of LAP+ cells abrogated the effect of Hsp65-producing L. lactis in EAE prevention and worsened disease in medium-fed mice. Thus, Hsp65-L.lactis seems to boost this critical regulatory circuit involved in controlling EAE development in mice. PMID:22939403

  4. Production of Ginsenoside F2 by Using Lactococcus lactis with Enhanced Expression of β-Glucosidase Gene from Paenibacillus mucilaginosus.

    Science.gov (United States)

    Li, Ling; Shin, So-Yeon; Lee, Soo Jin; Moon, Jin Seok; Im, Wan Taek; Han, Nam Soo

    2016-03-30

    This study aimed to produce a pharmacologically active minor ginsenoside F2 from the major ginsenosides Rb1 and Rd by using a recombinant Lactococcus lactis strain expressing a heterologous β-glucosidase gene. The nucleotide sequence of the gene (BglPm) was derived from Paenibacillus mucilaginosus and synthesized after codon optimization, and the two genes (unoptimized and optimized) were expressed in L. lactis NZ9000. Codon optimization resulted in reduction of unfavorable codons by 50% and a considerable increase in the expression levels (total activities) of β-glucosidases (0.002 unit/mL, unoptimized; 0.022 unit/mL, optimized). The molecular weight of the enzyme was 52 kDa, and the purified forms of the enzymes could successfully convert Rb1 and Rd into F2. The permeabilized L. lactis expressing BglPm resulted in a high conversion yield (74%) of F2 from the ginseng extract. Utilization of this microbial cell to produce F2 may provide an alternative method to increase the health benefits of Panax ginseng. PMID:26494255

  5. A food-grade fimbrial adhesin FaeG expression system in Lactococcus lactis and Lactobacillus casei.

    Science.gov (United States)

    Lu, W W; Wang, T; Wang, Y; Xin, M; Kong, J

    2016-03-01

    Enterotoxigenic Escherichia coli (ETEC) infection is the major cause of diarrhea in neonatal piglets. The fimbriae as colonizing factor in the pathogenesis of ETEC constitute a primary target for vaccination against ETEC. Lactic acid bacteria (LAB) are attractive tools to deliver antigens at the mucosal level. With the safety of genetically modified LAB in mind, a food-grade secretion vector (pALRc or pALRb) was constructed with DNA entirely from LAB, including the replicon, promoter, signal peptide, and selection marker alanine racemase gene (alr). To evaluate the feasibility of the system, the nuclease gene (nuc) from Staphylococcus aureus was used as a reporter to be expressed in both Lactococcus lactis and Lactobacillus casei. Subsequently, the extracellular secretion of the fimbrial adhesin FaeG of ETEC was confirmed by Western blot analysis. These results showed that this food-grade expression system has potential as the delivery vehicle for the safe use of genetically modified LAB for the development of vaccines against ETEC infection. PMID:26825016

  6. Preliminary X-ray crystallographic analysis of the d-xylulose 5-phosphate phosphoketolase from Lactococcus lactis

    International Nuclear Information System (INIS)

    The expression, purification, preliminary crystallization and crystallographic analysis of phosphoketolase from L. lactis ssp. lactis (strain IL 1403) are reported. Phosphoketolases are thiamine diphosphate-dependent enzymes which play a central role in the pentose-phosphate pathway of heterofermentative lactic acid bacteria. They belong to the family of aldehyde-lyases and in the presence of phosphate ion cleave the carbon–carbon bond of the specific substrate d-xylulose 5-phosphate (or d-fructose 6-phosphate) to give acetyl phosphate and d-glyceraldehyde 3-phosphate (or d-erythrose 4-phosphate). Structural information about phosphoketolases is particularly important in order to fully understand their mechanism as well as the steric course of phosphoketolase-catalyzed reactions. Here, the purification, preliminary crystallization and crystallographic characterization of d-xylulose 5-phosphate phosphoketolase from Lactococcus lactis are reported. The presence of thiamine diphosphate during purification was essential for the enzymatic activity of the purified protein. The crystals belonged to the monoclinic space group P21. Diffraction data were obtained to a resolution of 2.2 Å

  7. Biogenic amine production by Lactococcus lactis subsp. cremoris strains in the model system of Dutch-type cheese.

    Science.gov (United States)

    Flasarová, Radka; Pachlová, Vendula; Buňková, Leona; Menšíková, Anna; Georgová, Nikola; Dráb, Vladimír; Buňka, František

    2016-03-01

    The aim of this study was to compare the biogenic amine production of two starter strains of Lactococcus lactis subsp. cremoris (strains from the Culture Collection of Dairy Microorganisms - CCDM 824 and CCDM 946) with decarboxylase positive activity in a model system of Dutch-type cheese during a 90-day ripening period at 10°C. During ripening, biogenic amine and free amino acid content, microbiological characteristics and proximate chemical properties were observed. By the end of the ripening period, the putrescine content in both samples with the addition of the biogenic amine producing strain almost evened out and the concentration of putrescine was >800mg/kg. The amount of tyramine in the cheeses with the addition of the strain of CCDM 824 approached the limit of 400mg/kg by the end of ripening. In the cheeses with the addition of the strain of CCDM 946 it even exceeded 500mg/kg. In the control samples, the amount of biogenic amines was insignificant. PMID:26471528

  8. An evaluation and partial characterization of a bacteriocin produced by Lactococcus lactis subsp lactis ST1 isolated from goat milk.

    Science.gov (United States)

    Taheri, Parinaz; Samadi, Nasrin; Ehsani, Mohammad Reza; Khoshayand, Mohammad Reza; Jamalifar, Hossein

    2012-10-01

    A bacteriocin-like inhibitory substance producing Lactococcus lactis subsp lactis strain, ST1, isolated from goat milk of Iranian origin and with broad spectrum of activity and desirable technical properties was used for evaluating some futures of bacteriocin inhibitory activity. Cell growth and bacteriocin production studies were carried out in MRS medium incubated statically under uncontrolled pH condition. The antibacterial activity presented a primary metabolite pattern and showed a rapid decrease at the stationary phase. Microaerobiosis and capnophily growth conditions resulted in higher bacteriocin production while aerobiosis showed negative effect on both cell growth and bacteriocin production. Bacteriocin production, on the other hand, was favored in MRS broth (pH; 6.5) inoculated with 0.1 ml l(-1) fresh culture when incubation was carried out at 30 °C. This indicated that the conditions resulted in higher levels of growth were frequently favoring bacteriocin production by ST1 as well. Decrease in activity, at the stationary growth phase, was much pronounced in favored growth condition. Nutrient depletion, deferent effect of low pH on bacteriocin production and/or protein degradation seemed more responsible for this phenomenon. The study also provided further data on new method for bacteriocin release from the cell wall of producer. It was clearly shown that both heating and ultrasound shock for 5 min at pH 2 could increase bacteriocin activity significantly. The release was more pronounced in the presence of 0.5% Tween80. PMID:24031976

  9. An evaluation and partial characterization of a bacteriocin produced by Lactococcus lactis subsp lactis ST1 isolated from goat milk

    Directory of Open Access Journals (Sweden)

    Parinaz Taheri

    2012-12-01

    Full Text Available A bacteriocin-like inhibitory substance producing Lactococcus lactis subsp lactis strain, ST1, isolated from goat milk of Iranian origin and with broad spectrum of activity and desirable technical properties was used for evaluating some futures of bacteriocin inhibitory activity. Cell growth and bacteriocin production studies were carried out in MRS medium incubated statically under uncontrolled pH condition. The antibacterial activity presented a primary metabolite pattern and showed a rapid decrease at the stationary phase. Microaerobiosis and capnophily growth conditions resulted in higher bacteriocin production while aerobiosis showed negative effect on both cell growth and bacteriocin production. Bacteriocin production, on the other hand, was favored in MRS broth (pH; 6.5 inoculated with 0.1 ml l-1 fresh culture when incubation was carried out at 30 °C. This indicated that the conditions resulted in higher levels of growth were frequently favoring bacteriocin production by ST1 as well. Decrease in activity, at the stationary growth phase, was much pronounced in favored growth condition. Nutrient depletion, deferent effect of low pH on bacteriocin production and/or protein degradation seemed more responsible for this phenomenon. The study also provided further data on new method for bacteriocin release from the cell wall of producer. It was clearly shown that both heating and ultrasound shock for 5 min at pH 2 could increase bacteriocin activity significantly. The release was more pronounced in the presence of 0.5% Tween80.

  10. Antimicrobial activity of the Nisin Z producer Lactococcus lactis subsp. lactis Lc08 against Listeria monocytogenes in skim milk

    Directory of Open Access Journals (Sweden)

    L.M. Perin

    2013-10-01

    Full Text Available The presented study aimed to verify the effect of different pH values, enzyme solutions and heat treatments on the antimicrobial activity of the bacteriocinogenic strain Lactococcus lactis subsp. lactis Lc08 and to test their antimicrobial activity against Listeria monocytogenes in reconstituted skim milk at refrigeration temperatures. This strain was previously described as a nisin Z producer and capable of inhibiting L. monocytogenes growth in in vitro tests. The antimicrobial activity of the bacteriocin cell-free supernatant of Lc08 was sensitive to enzyme treatments (except papain. The pH values and heating (65ºC for 30min, 75ºC for 15s had no apparent effect on the antimicrobial activity of the bacteriocin produced by Lc08. Only treatment at autoclave conditions result in loss of their antimicrobial activity. Lc08 presented antimicrobial activity against L. monocytogenes in the milk system after 12h at 25ºC. No effect was found at 7ºC. The results show the application viability of the Lc08 in food systems as a biopreservative against L. monocytogenes.

  11. Induction of a stress response in Lactococcus lactis is associated with a resistance to ribosomally active antibiotics.

    Science.gov (United States)

    Dorrian, James M; Briggs, Deborah A; Ridley, Michael L; Layfield, Robert; Kerr, Ian D

    2011-11-01

    The acquisition of multidrug resistance in bacteria underlies the failure of antimicrobial therapy, and the emergence of pathogens that are resistant to almost the entire armoury of antibiotics. Among the proteins that can mediate or contribute to the drug-resistance profile in Gram-positive bacteria is a subset of ATP-binding cassette proteins that are comprised of a tandem-repeated nucleotide-binding domain. In this study, we expressed one of these NBD(2) proteins, LmrC, in an antibiotic-sensitive Gram-positive host strain (Lactococcus lactis) and demonstrated the acquisition of resistance to ribosomally active antibiotics. Mutation of key catalytic residues suggested that the resistance profile was the result of a cellular response, rather than being a function of the NBD(2) protein itself. This observation was confirmed by 2D SDS/PAGE, which demonstrated that the expression of the NBD(2) protein induced a stress response in L. lactis. A model combining this stress response induction and the acquisition of antibiotic resistance is proposed. PMID:21848804

  12. Improvement in lactic acid production from starch using alpha-amylase-secreting Lactococcus lactis cells adapted to maltose or starch.

    Science.gov (United States)

    Okano, Kenji; Kimura, Sakurako; Narita, Junya; Fukuda, Hideki; Kondo, Akihiko

    2007-07-01

    To achieve direct and efficient lactic acid production from starch, a genetically modified Lactococcus lactis IL 1403 secreting alpha-amylase, which was obtained from Streptococcus bovis 148, was constructed. Using this strain, the fermentation of soluble starch was achieved, although its rate was far from efficient (0.09 g l(-1) h(-1) lactate). High-performance liquid chromatography revealed that maltose accumulated during fermentation, and this was thought to lead to inefficient fermentation. To accelerate maltose consumption, starch fermentation was examined using L. lactis cells adapted to maltose instead of glucose. This led to a decrease in the amount of maltose accumulation in the culture, and, as a result, a more rapid fermentation was accomplished (1.31 g l(-1) h(-1) lactate). Maximum volumetric lactate productivity was further increased (1.57 g l(-1) h(-1) lactate) using cells adapted to starch, and a high yield of lactate (0.89 g of lactate per gram of consumed sugar) of high optical purity (99.2% of L: -lactate) was achieved. In this study, we propose a new approach to lactate production by alpha-amylase-secreting L. lactis that allows efficient fermentation from starch using cells adapted to maltose or starch before fermentation. PMID:17384945

  13. Oral Vaccination with the Porcine Rotavirus VP4 Outer Capsid Protein Expressed by Lactococcus lactis Induces Specific Antibody Production

    Directory of Open Access Journals (Sweden)

    Yi-jing Li

    2010-01-01

    Full Text Available The objective of this study to design a delivery system resistant to the gastrointestinal environment for oral vaccine against porcine rotavirus. Lactococcus lactis NZ9000 was transformed with segments of vP4 of the porcine rotavirus inserted into the pNZ8112 surface-expression vector, and a recombinant L. lactis expressing VP4 protein was constructed. An approximately 27 kDa VP4 protein was confirmed by SDS-PAGE , Western blot and immunostaining analysis. BALB/c mice were immunized orally with VP4-expression recombinant L. lactis and cellular, mucosal and systemic humoral immune responses were examined. Specific anti-VP4 secretory IgA and IgG were found in feces, ophthalmic and vaginal washes and in serum. The induced antibodies demonstrated neutralizing effects on porcine rotavirus infection on MA104 cells. Our findings suggest that oral immunization with VP4-expressing L. lactis induced both specific local and systemic humoral and cellular immune responses in mice.

  14. Construction of a new shuttle vector for DNA delivery into mammalian cells using non-invasive Lactococcus lactis.

    Science.gov (United States)

    Yagnik, Bhrugu; Padh, Harish; Desai, Priti

    2016-04-01

    Use of food grade Lactococcus lactis (L. lactis) is fast emerging as a safe alternative for delivery of DNA vaccine. To attain efficient DNA delivery, L. lactis, a non-invasive bacterium is converted to invasive strain either by expressing proteins like Internalin A (InlA) or Fibronectin binding protein A (FnBPA) or through chemical treatments. However the safety status of invasive L. lactis is questionable. In the present report, we have shown that non-invasive L. lactis efficiently delivered the newly constructed reporter plasmid pPERDBY to mammalian cells without any chemical enhancers. The salient features of the vector are; I) Ability to replicate in two different hosts; Escherichia coli (E. coli) and Lactic Acid Bacteria (LAB), II) One of the smallest reporter plasmid for DNA vaccine, III) Enhanced Green Fluorescence Protein (EGFP) linked to Multiple Cloning Site (MCS), IV) Immunostimulatory CpG motifs functioning as an adjuvant. Expression of EGFP in pPERDBY transfected CHO-K1 and Caco-2 cells demonstrates its functionality. Non-invasive r-L. lactis was found efficient in delivering pPERDBY to Caco-2 cells. The in vitro data presented in this article supports the hypothesis that in the absence of invasive proteins or relevant chemical treatment, L. lactis was found efficient in delivering DNA to mammalian cells. PMID:26655884

  15. Resistencia bacteriana a antibióticos en el Hospital San Juan de Dios, 1995-1999

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    Ricardo Boza-Cordero

    2001-07-01

    Full Text Available Justificación: La resistencia a antibióticos por parte de bacterias patógenas es un grave problema mundial. Su control ha sido difícil pero deben hacerse todos los esfuerzos por realizarlo. Conocer la epidemiología en las diferentes regiones y países se constituye en parte importante de este control. Este estudio se llevóa cabo con el fin de analizar el comportamiento de la resistencia en el hospital San Juan de Dios, centro de atención nacional de adultos de aproximadamente 700 camas. Materiales y métodos: El estudio se basóen los datos obtenidos en los años 1995-1999 ya que en 1995 se inició el empleo de equipo automatizado VITEK® en este hospital. Se analizaron 2817 aislamientos de cocos Gram positivos (CGP y 7626 de bacilos Gram negativos (BGN obtenidos de todos los sitios anatómicos y fluidos corporales. Por ser un estudio retrospectivo, no se pudo definir cuáles aislamientos provenían de infecciones intra o extrahospitalarias. Resultados: Staphylococcus aureus fue el CGP más frecuentemente aislado, mientras que el BGN más frecuente fue Escherichia coli. En más de 90% de los estafilococos aislados se demostróresistencia a la penicilina. La resistencia a la oxacilina en S.aureus se incrementóde 35% en 1995 a 52% en 1999 mientras que en los estafilococos coagulasa negativa, pasóde 70 a 77% en el mismo periodo. La resistencia a la cefalotina en S.aureus pasóde 35 a 50% mientras que en los estafilococos coagulasa negativa pasóde 65 a 76%. La resistencia a la clindamicina en ambos grupos bacterianos se mantuvo relativamente estable. No se demostróresistencia a la vancomicina. La mayoría de los enterococos aislados correspondieron a E. faecalis, 75% de ellos fueron sensibles a la penicilina y 50% no mostraron sinergismo de la gentamicina con los antibióticos que actúan sobre la pared. No se encontraron enterococos vancomicina resistentes. En las enterobacterias se observó un aumento importante de la resistencia a

  16. Advances in diagnostic tests for bacterial STDs Avances en las pruebas diagnósticas de ETS bacterianas

    Directory of Open Access Journals (Sweden)

    Stephen A Morse

    2003-01-01

    Full Text Available Because of their asymptomatic nature and nonspecific symptoms, laboratory tests are often required to diagnose a sexually transmitted infection. Over the past few years, there have been advances in technology, such as the development of nucleic acid amplification assays, which have improved our ability to diagnose infections caused by Chlamydia trachomatis. The finding that nucleic acid amplification tests can detect more infected individuals and are useful in screening low prevalence populations, has led to the development of strategies designed to reduce the cost of these assays without significantly impacting their sensitivity. The development of new tests for the diagnosis of syphilis has gained momentum from the report of a synthetic VDRL antigen, which will result in better nontreponemal antibody tests for syphilis. In spite of the completion of the genome sequence of Treponema pallidum and its annotation, we are still unable to cultivate this microorganism in vitro. However, the molecular revolution has resulted in the development of PCR assays for detecting Treponema pallidum in various types of clinical specimens, and to the production of recombinant antigens for use in tests that detect treponemal-specific antibodies. Further research will improve the availability of low cost, sensitive tests for the diagnosis of sexually transmitted infections.Las pruebas de laboratorio son necesarias a menudo para el diagnóstico de las infecciones transmitidas sexualmente, debido a la naturaleza asintomática o a la presencia de síntomas inespecíficos de esas infecciones. En este sentido, durante los años relativamente recientes se han registrado importantes avances tecnológicos, como por ejemplo los ensayos de amplificación de ácidos nucleicos que han permitido una mejora en la posibilidad de diagnosticar las infecciones causadas por Chlamydia trachomatis. El descubrimiento de que las pruebas de amplificación de ácidos nucleicos permiten

  17. Portable exhausters POR-004 SKID B, POR-005 SKID C, POR-006 SKID D storage plan

    International Nuclear Information System (INIS)

    This document provides a storage plan for portable exhausters POR-004 SKID B, POR-005 SKID C, AND POR-006 SKID D. The exhausters will be stored until they are needed by the TWRS (Tank Waste Remediation Systems) Saltwell Pumping Program. The storage plan provides criteria for portable exhauster storage, periodic inspections during storage, and retrieval from storage

  18. Pasteurización de calostro: efecto sobre la carga bacteriana y la concentración de inmunoglobulinas G.

    Directory of Open Access Journals (Sweden)

    Jorge A. Elizondo-Salazar

    2008-09-01

    Full Text Available La pasteurización de calostro a nivel de finca ha recibido una considerable atención en los últimos años, con el fin de reducir agentes patógenos bacterianos. La adopción de esta práctica a nivel de finca ha reportado resultados significativos en la salud de las terneras y en los ingresos económicos de los productores. Sin embargo, existe poca información con respecto al efecto que tiene la pasteurización sobre la concentración de inmunoglobulinas G (IgG. Por esta razón, se llevó a cabo una investigación con el objetivo de determinar el efecto de la pasteurización (baja temperatura-largo tiempo sobre el nivel bacterial y la concentración de IgG en calostro bovino. Se colectó calostro de primer ordeño de 28 vacasHolstein. Cada muestra se agitó completamente y dos sub-muestras de 10- mL fueron analizadas. La primera sub-muestra sirvió como control mientras que la segunda fue calentada a 62.8 °C por 30 min. Las muestras de calostro tratadas y sin tratar fueron analizadas para determinar el conteo estándar de placa (CEP, conteo preliminar de incubación (CPI, conteo de coliformes (CC, conteo de no-coliformes (CNC, conteo de estreptococos ambientales (CEA y conteo de Staphylococcus aureus (CSA. Las concentraciones de IgG1 e IgG2 fueron medidas utilizando la técnica de inmunodifusión radial. Los resultados del estudio mostraron que la pasteurización tuvo como resultado una reducción significativa (P < 0.01 en los niveles de CEP, CC, CNC, CEA y CSA. La pasteurización tuvo también como resultado la desnaturalización del 14% de las IgG totales en elcalostro.

  19. Resistencia bacteriana a los antibióticos en la Unidad de Cuidados Intensivos, Hospital de Caldas, 1992-1994.

    Directory of Open Access Journals (Sweden)

    Eduardo León Jaramillo V.

    2009-09-01

    Full Text Available Se describe y analiza el comportamiento de los microorganismos más frecuentes en la Unidad de Cuidados Intensivos (UCI, del Hospital de Caldas (HC, y su sensibilidad/resistencia a los antibióticos, según los antibiogramas hechos por el laboratorio clínico del HC, entre 1992 y 1994. Enterobacter aerogenes fue el germen más común en la UCI. Staphylococcus dnasa negativo presentó frecuencia creciente, a través de los años del estudio. La UCI aportó 39.6% de Pseudomonas del HC. El germen más frecuente en líquido peritoneal, secreciones traqueobronquiales y orina, fue E. aerogenes; en las puntas de los catéteres venosos, Staphylococcus dnasa negativo; y, en los tubos de tórax, P. aeruginosa. La resistencia a los antibióticos en la UCI fue casi el doble a la de otros servicios del HC. Las cepas de estafilococos meticilino resistentes, en la UCI, superan 60% y empiezan a aparecer cepas resistentes a la vancomicina. Pseudomonas aeruginosa fue muy resistente tanto a los antibióticos tradicionales como a los modernos antipseudomonas. Imipenem fue el antibiótico más eficaz contra Gram negativos aerobios, incluida P. aeruginosa.

  20. Ojo por ojo

    OpenAIRE

    Ramón del Llano Ibáñez

    2011-01-01

    Nuestro pan y café de la mañana se acompaña de noticias alrededor de ejecuciones, de fosas clandestinas, de batallas del ejército, de balaceras en las calles y civiles caídos por balas perdidas : la muerte acompaña nuestro desayuno. Las calaveras de azúcar del día de muertos hoy son calaveras reales y amargas : 40 000 muertos en cuatro años después que el Presidente Calderón declaró la guerra a los narcotraficantes. Se han apresado a varios e importantes miembros de los cárteles : pero han si...

  1. A vida por segundos

    OpenAIRE

    Silva, Pedro Miguel Paiva da

    2010-01-01

    A Enfermagem regista evolução a vários níveis, o que torna imperioso reconhecer como de significativo valor o papel do enfermeiro. A Especialização em Enfermagem Médico-Cirúrgica visa o desenvolvimento de competências para a assistência de Enfermagem avançada ao doente adulto e idoso em estado crítico, revelando uma maior apropriação, por parte dos enfermeiros especialistas de uma concepção de cuidados sustentada numa conceptualização científica dos cuidados, daí infere-se o contributo num...

  2. Ojo por ojo

    Directory of Open Access Journals (Sweden)

    Ramón del Llano Ibáñez

    2011-12-01

    Full Text Available Nuestro pan y café de la mañana se acompaña de noticias alrededor de ejecuciones, de fosas clandestinas, de batallas del ejército, de balaceras en las calles y civiles caídos por balas perdidas : la muerte acompaña nuestro desayuno. Las calaveras de azúcar del día de muertos hoy son calaveras reales y amargas : 40 000 muertos en cuatro años después que el Presidente Calderón declaró la guerra a los narcotraficantes. Se han apresado a varios e importantes miembros de los cárteles : pero han si...

  3. Onicomicosis por hongos fuliginosos

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    Ingrid Salas-Campos

    2009-12-01

    Full Text Available Justificación y objetivo: Las infecciones de las uñas producidas por hongos son un problema de frecuente consulta dermatológica. Los dermatofitos son la mayor causa de estas infecciones, siendo Trichophyton rubrum el agente etiológico más importante. Sin embargo, los hongos no dermatofitos, tanto hialinos como fuliginosos, pueden ser también responsables de estos cuadros clínicos. Debido a que estos hongos tienden a ser resistentes a los antimicóticos comúnmente usados para tratar las onicomicosis, es que se hace indispensable que el clínico se asegure por medio de un examen de laboratorio de la etiología de la infección. Métodos: Se estudiaron tres casos de pacientes con lesiones en uñas, que acudieron al laboratorio de Micología Médica, Facultad de Microbiología, Universidad de Costa Rica. Se realizaron los análisis de rutina para aislamiento e identificación de hongos patógenos. Resultados: En los tres casos estudiados se observó al examen directo micelio fuliginoso. En uno de los casos se aisló e identificó Scytalidium dimidiatum, hongo que presenta resistencia a los antifúngicos utilizados para tratar onicomicosis. Conclusión. El reporte de hongos diferentes a los dermatofitos como agentes etiológicos de onicomicosis es importante para que el médico pueda elegir el tratamiento más recomendado para este tipo de infección.

  4. Desarrollo de un chip de DNA para la detección de dianas génicas bacterianas de interés en el campo de la acuicultura

    OpenAIRE

    A. Labella; Trento, I.; Ghidini, V.; Gennari, M; Castro, D.; Borrego, Juan José; Lleò, M M

    2014-01-01

    La adquisición bacteriana de multiresistencias frente a antimicrobianos es un fenómeno cada vez más preocupante, con gran repercusión para la salud pública, pero también con impacto en sectores de la producción animal, como es el caso particular de la acuicultura. Entre los años 2010-2012, como objetivo de un Proyecto de Investigación Italiano (Progetti d’interesse Nazionale, PRIN), se realizaron diferentes campañas de muestreo a lo largo de la costa del mar Adriático, en centros dedicados a ...

  5. Infecção bacteriana na leishmaniose cutânea: padrão bacteriano e sensibilidade a antibióticos Bacterial infection in cutaneous leishmaniasis: bacterial pattern and sensitivity to antibiotics

    OpenAIRE

    Eloísa da Graça do Rosário Gonçalves; Stelito Assis dos Reis Filho; Eurípedes Gomes de Oliveira; André Luiz Neves Pareira; Antonio Rafael da Silva; Jackson Maurício Lopes Costa

    2009-01-01

    Foi estudada a flora bacteriana em úlceras leishmanióticas, destacando-se o encontro das espécies aeróbicas Staphylococus aureus e Pseudomonas aeruginosa. O estudo da sensibilidade destas espécies a antibióticos mostrou sensibilidade à vancomicina, à amicacina e ao cloranfenicol em 100% dos isolados testados de Staphylococus aureus e à amicacina, à gentamicina e à tobramicina em 100% dos isolados testados de Pseudomonas aeruginosa. Estas espécies foram, em geral, resistentes às penicilinas e ...

  6. Peritonite bacteriana espontânea na cirrose hepática: prevalência, fatores preditivos e prognóstico Spontaneous bacterial peritonitis in hepatic cirrhosis: prevalence, predictive factors and prognosis

    OpenAIRE

    F.A.F. Figueiredo; H.S.M. Coelho; J.A.S. Soares

    1999-01-01

    OBJETIVO: Investigar prevalência, fatores preditivos e prognóstico dos episódios de Peritonite Bacteriana Espontânea (PBE) na cirrose hepática. METODOLOGIA: Estudamos, prospectivamente, 143 pacientes com cirrose hepática, ambulatoriais ou internados, que foram atendidos nos Serviços de Clínica Médica do HUCFF e de Gastroenterologia do HUPE no período de janeiro/95 a janeiro/96. Estes pacientes foram submetidos a questionário, exame físico, colheita de sangue e paracentese abdominal com colhei...

  7. Investigation of bacterial microbiota and risk factors in dogs with external ocular diseases from Bandeirantes, Paraná State, BrazilInvestigação da microbiota bacteriana e associações de risco em cães com afecções oculares externas atendidos em Bandeirantes, Paraná, Brasil

    Directory of Open Access Journals (Sweden)

    João Luis Garcia

    2012-02-01

    bilaterais, e 120 cães sem doenças oculares (grupo controle, foram atendidos entre 08/2008 a 07/2009 no Hospital Veterinário da Universidade Estadual do Norte do Paraná. As amostras coletadas do saco conjuntival inferior foram semeadas em ágar sangue e MacConkey e incubadas em aerobiose a 37ºC por até 120 horas. Após a identificação presuntiva, as espécies bacterianas foram identificadas pelos sistemas APISTAPH (bio- Merieux, Inc., API 20 STREP (bio-Merieux, Inc. e BACTRAY (Laborclin, ltda. e semeadas em agar Mueller-Hinton, para determinação da sensibilidade por meio da técnica de difusão de discos. Para as associações de risco, os proprietários dos 158 cães responderam um questionário com variáveis epidemiológicas. Das 63 amostras, houve crescimento de microrganismos em 46 (73,02%, com isolamento de um microrganismo em 42 amostras e de dois microrganismos em quatro. Bactérias Gram positivas corresponderam a 76% dos isolamentos, Gram negativas a 20% e fungos leveduriformes a 4%. Staphylococcus spp totalizaram 66% dos isolamentos, sendo S. aureus (24% e S. intermedius (24% as mais prevalentes. Exceto S. intermedius (91,67% e S. epidermidis (66,67%, as espécies bacterianas isoladas apresentaram resistência de 100% às sulfonamidas. Os S. aureus isolados apresentaram sensibilidade de 91,67% para cloranfenicol, tobramicina e amoxicilina/clavulanato, e o mesmo percentual de resistência para tetraciclina. Os S. intermedius apresentaram 100% de sensibilidade para amoxicilina/clavulanato e 91,67% para gentamicina e resistência de 75% para tetraciclina e ceftriaxona. As associações de risco para as doenças oculares externas foram recidivas clínicas (OR=59,50, 7,29

  8. Aggregation of plaque disclosing agent in a dentifrice Incorporação de evidenciador de placa bacteriana a um dentifrício

    Directory of Open Access Journals (Sweden)

    Débora Dias da Silva

    2004-06-01

    Full Text Available Dental plaque removal is an important issue in health promotion. Toothbrushing is one of the main methods employed for such purpose, since it can prevent dental caries by means of the fluoride present in the dentifrice. Dentifrices might contain plaque disclosing agents and thus allow dental plaque observation. The aim of this study was to assess whether utilization of a plaque disclosing agent interfered with plaque removal among adolescents, as well as the difference between utilization of erythrosine tablets and dentifrices containing plaque disclosing agent. The sample was composed of 62 students from Piracicaba, SP, Brazil, aged 12 to 14 years old, divided into 3 groups: G1 or control group (toothbrushing without plaque disclosure; G2 (plaque disclosing with an erythrosine tablet and toothbrushing and G3 (toothbrushing with dentifrice containing plaque disclosing agent. After toothbrushing, disclosure of the remaining dental plaque was performed in all groups with a fuchsin tablet and measured through the Simplified Oral Health Assessment Index (OHI-S, in two stages with a 2-month interval between them. The analysis of variance (ANOVA showed that there was no difference in the OHI-S index between the groups (p>0.05, however the G3 displayed a higher proportion of students with plaque reduction (23% than G2 (21%, besides the smallest difference in the mean remaining dental plaque. There was no difference between groups; however, it was suggested that the dentifrice with plaque disclosing agent had positive results in relation to the erythrosine tablet, even though the small sample size may have interfered with the results, indicating the need of complementary studies.Na promoção de saúde, a remoção da placa bacteriana é um fator importante e um dos métodos que incentivam sua remoção, é a escovação dentária, podendo-se prevenir a cárie dental através do íon flúor dos dentifrícios. Estes podem conter evidenciadores

  9. INFLUENCIA DE BACTERIOCINAS PRODUCIDAS POR Lactobacillus plantarum LPBM10 SOBRE LA VIDA ÚTIL DE FILETES DEL HÍBRIDO DE CACHAMA Piaractus brachypomus x Colossoma macropomum EMPACADO AL VACÍO: INFLUENCE OF BACTERIOCINS PRODUCED BY Lactobacillus plantarum LPBM10 ON SHELF LIFE OF CACHAMA HYBRID FILLETS Piaractus rachypomus x Colossoma macropomum VACUUM PACKAGED

    OpenAIRE

    Héctor SUÁREZ M.; Alicia DE FRANCISCO; Luiz H. Beirão

    2008-01-01

    El uso de algunos de los géneros de las bacterias ácidolácticas (BAL) como Lactobacillus, Leuconostoc, Lactococcus, o sus productos antimicrobianos como las bacteriocinas, son alternativas en la biopreservación de productos mínimamente procesados que son mantenidos bajo refrigeración. El potencial de biopreservación de un extracto crudo de bacteriocinas producidas por una cepa nativa de Lactobacillus plantarum LPBM10, se evalúa sobre filetes de híbrido de cachama Piaractus brachypomus x Colos...

  10. Contaminação bacteriana e fúngica de canudos de refrigerantes e seus recipientes em lanchonetes de município do interior de São Paulo Fungal and bacterial contamination of drinking straws and their containers in snack bars in a municipality of São Paulo state, Brazil

    Directory of Open Access Journals (Sweden)

    Francisco Rafael Martins Soto

    2009-12-01

    Full Text Available OBJETIVO: Avaliar o nível de contaminação bacteriana e fúngica de canudos de refrigerantes e seus recipientes em 30 lanchonetes do Município de Ibiúna (SP, correlacionando com as condições de higiene, processos e métodos de desinfecção destes estabelecimentos. MÉTODOS: Foram colhidas três amostras por estabelecimento nas embalagens fechadas, em recipientes e swab em toda a superfície de contato. Foi aplicado um questionário a fim de avaliar: a empresa fornecedora dos canudos, higienização, freqüência e desinfecção, e foram efetuadas inspeções sanitárias nos estabelecimentos. Para as análises microbiológicas foi utilizada a técnica de lavagem superficial e semeadura em meios, para contagem de bactérias mesófilas. As amostras turvas foram semeadas em meios de cultura para: Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, Enterococcus, coliformes totais e/ou termotolerantes. RESULTADOS: Dentre os microorganismos isolados nas amostras dos canudos nos recipientes foi detectado Bacillus cereus em 36,6%, Enterococo spp. em 3,3%. O Bacillus cereus foi isolado em 46,6% nos swabs dos recipientes, e em 13,3%, Enterococos. Na análise de associação do nível de contaminação microbiana de canudos de refrigerantes e seus recipientes com as condições de higiene, os processos de higienização e a desinfecção dos estabelecimentos, não foi identifica significância estatística (p>0,05. CONCLUSÃO: O Bacillus cereus foi o microorganismo que prevaleceu nas embalagens íntegras dos canudos, nos seus recipientes e no swab das superfícies. Não foi comprovada a associação de fatores de risco de contaminação bacteriana e fúngica.OBJECTIVE: The objective of this study was to evaluate the fungal and bacterial contamination level of drinking straws and their containers of thirty snack bars at the municipality of Ibiuna (SP, Brazil and to correlate these data with conditions of hygiene and the processes and

  11. Efeito de produtos químicos sobre a mancha bacteriana (Xanthomonas perforans e na ativação de proteínas relacionadas à patogênese em tomateiro Effect of chemicals on the bacterial spot (Xanthomonas perforans and the activation of pathogenesis-related proteins in tomato

    Directory of Open Access Journals (Sweden)

    Adriana Terumi Itako

    2012-08-01

    Full Text Available O presente trabalho teve por objetivo avaliar a ação de fungicidas e antibiótico no controle da mancha bacteriana (Xanthomonas perforans em tomate e na ativação de proteínas relacionada à patogênese. Para avaliação da severidade da doença foi utilizada o híbrido de tomate "AP529". Os tratamentos consistiram da pulverização com acibenzolar-S-metil, fluazinam, piraclostrobina, piraclostrobina + methiran, oxicloreto de cobre, oxicloreto de cobre + mancozeb e oxitetraciclina, além de uma testemunha inoculada e uma testemunha não inoculada. Após três dias do tratamento, as plantas foram inoculadas com a bactéria X. perforans (10(6 UFC/mL. Discos foliares foram coletadas para avaliação das enzimas peroxidase, polifenoloxidase, β-1,3 glucanase, fenilalanina amonia liase e protease. Com os dados da severidade foi calculada a Área Abaixo da Curva de Progresso da Doença (AACPD. Todos os tratamentos apresentaram redução da AACPD em relação à testemunha inoculada. Os fungicidas acibenzolar-S-metil, piraclostrobina e piraclostrobina + methiran tiveram resultados mais satisfatórios na redução da severidade da mancha bacteriana em tomateiro. Os produtos à base de piraclostrobina juntamente com acibenzolar-S-metil induziram as atividades enzimáticas de peroxidase, polifenoloxidase e β-1,3 glucanase, evidenciando que estes produtos podem estar relacionados à indução de resistência à mancha bactéria em plantas de tomateiro.The present study aims to evaluate the effect of fungicides and antibiotics to control bacterial spot (Xanthomonas perforans in tomato, and the activation of pathogenesis-related proteins. Hybrid tomato AP 529 was used to assess the severity of disease. The treatments consisted of spraying with acibenzolar-S-methyl, fluazinam, pyraclostrobin, pyraclostrobin + methiran, copper oxychloride, copper oxychloride and mancozeb + oxytetracycline, and inoculated and non-inoculated controls. After three days of

  12. Molecular analysis of the bacterial diversity in a specialized consortium for diesel oil degradation Análise molecular da diversidade bacteriana de um consórcio degradador de óleo diesel

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    Douglas Antonio Alvaredo Paixão

    2010-06-01

    desse produto contribuem significativamente para derrames acidentais, ocasionando sérios problemas ecológicos no solo e água, alterando assim toda a diversidade microbiológica do ambiente. A estratégia de clonagem e sequenciamento do gene 16S rRNA é uma das técnicas moleculares que permitem estimar e comparar a diversidade microbiana de diferentes amostras ambientais, sejam elas impactadas ou não. O objetivo deste trabalho foi estimar a diversidade de microrganismos pertencentes ao domínio Bacteria em um consórcio degradador de óleo diesel por meio de sequenciamento parcial do gene 16S rRNA. Após extração do DNA metagenômico, o material foi amplificado por reação de PCR com oligonucleotídeos iniciadores específicos para o gene 16S rRNA. Os produtos da reação de PCR foram clonados em vetor pGEM T Easy (Promega e transformados em células competentes de Escherichia coli. O sequenciamento parcial dos clones foi feito com oligonucleotídeos universais do vetor. A biblioteca obtida gerou 431 clones. Todos os clones mostraram similaridade com o filo Proteobacteria, onde as Gammaproteobacteria compreenderam o grupo de maior representatividade, com 49,8 % dos clones, seguida das Alphaproteobacteira, com 44,8 %, e das Betaproteobacteria, com 5,4 %. O gênero Pseudomonas destacou-se como representante com maior frequência de clones na biblioteca, seguido pelos gêneros Parvibaculum e Sphingobium. Após o sequenciamento parcial do gene 16S rRNA, a diversidade bacteriana do consórcio foi estimada utilizando-se o software DOTUR. Essas sequências, quando comparadas com as do banco do National Center for Biotechnology Information (NCBI, mostraram grande correlação entre os dados gerados pelo software utilizado e aqueles depositados no NCBI.

  13. Integration and gene replacement in the Lactococcus lactis lac operon: induction of a cryptic phospho-beta-glucosidase in LacG-deficient strains.

    OpenAIRE

    Simons, G.; Nijhuis, M.; de Vos, W M

    1993-01-01

    Insertions, replacement mutations, and deletions were introduced via single or double crossover recombination into the lacE (enzyme IIlac) and lacG (phospho-beta-galactosidase) genes of the Lactococcus lactis chromosomal lacABCDFEGX operon. LacG production was abolished in strains missing the lacG gene or carrying multicopy insertions in the lacE gene that affected expression of the lacG gene. However, these LacG-deficient strains could still ferment lactose slowly and were found to contain a...

  14. Lactococcus garvieae endocarditis in a native valve identified by MALDI-TOF MS and PCR-based 16s rRNA in Spain: A case report

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    V. Heras Cañas

    2015-05-01

    Full Text Available Lactococcus garvieae is a Gram-positive, catalase negative coccus arranged in pairs or short chains, well-known as a fish pathogen. We report a case of Infective Endocarditis (IE by L. garvieae in a native valve from a 68-year-old male with unknown history of contact with raw fish and an extensive history of heart disease. This case highlights the reliability of MALDI-TOF MS compared to conventional methods in the identification of rare microorganisms like this.

  15. Exopolysaccharide Biosynthesis in Lactococcus lactis NIZO B40: Functional Analysis of the Glycosyltransferase Genes Involved in Synthesis of the Polysaccharide Backbone

    OpenAIRE

    Kranenburg, Richard van; van Swam, Iris I.; Marugg, Joey D.; Kleerebezem, Michiel; de Vos, Willem M.

    1999-01-01

    We used homologous and heterologous expression of the glycosyltransferase genes of the Lactococcus lactis NIZO B40 eps gene cluster to determine the activity and substrate specificities of the encoded enzymes and established the order of assembly of the trisaccharide backbone of the exopolysaccharide repeating unit. EpsD links glucose-1-phosphate from UDP-glucose to a lipid carrier, EpsE and EpsF link glucose from UDP-glucose to lipid-linked glucose, and EpsG links galactose from UDP-galactos...

  16. The crystal structure of Lactococcus lactis dihydroorotate dehydrogenase A complexed with the enzyme reaction product throws light on its enzymatic function

    DEFF Research Database (Denmark)

    Rowland, Paul; Bjørnberg, Olof; Nielsen, Finn S.; Jensen, Kaj Frank; Larsen, Sine

    1998-01-01

    Dihydroorotate dehydrogenases (DHODs) catalyze the oxidation of (S)-dihydroorotate to orotate, the fourth step and only redox reaction in the de novo biosynthesis of pyrimidine nucleotides. A description is given of the crystal structure of Lactococcus lactis dihydroorotate dehydrogenase A (DHODA......) complexed with the product of the enzyme reaction orotate. The structure of the complex to 2.0 A resolution has been compared with the structure of the native enzyme. The active site of DHODA is known to contain a water filled cavity buried beneath a highly conserved and flexible loop. In the complex the...

  17. Identification of a Conserved Sequence in Flavoproteins Essential for the Correct Conformation and Activity of the NADH Oxidase NoxE of Lactococcus lactis ▿ †

    OpenAIRE

    Tachon, Sybille; Chambellon, Emilie; Yvon, Mireille

    2011-01-01

    Water-forming NADH oxidases (encoded by noxE, nox2, or nox) are flavoproteins generally implicated in the aerobic survival of microaerophilic bacteria, such as lactic acid bacteria. However, some natural Lactococcus lactis strains produce an inactive NoxE. We examined the role of NoxE in the oxygen tolerance of L. lactis in the rich synthetic medium GM17. Inactivation of noxE suppressed 95% of NADH oxidase activity but only slightly affected aerobic growth, oxidative stress resistance, and NA...

  18. Efecto del pH, cepa bacteriana y tipo de muestra, en la detección microbiológica, de ácido oxolínico y oxitetraciclina en peces Influence of pH, microbial strain and sample type, on the microbiological detection of oxolinic acid and oxitetraciclyne in fish

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    ERIKA GESCHE

    2001-01-01

    Full Text Available Junto al desarrollo de la salmonicultura, surge la necesidad de aplicación de antimicrobianos para el control de enfermedades de los peces, aumentando el riesgo de la presencia de residuos en el producto destinado al consumo. Con la finalidad de aportar antecedentes a la detección microbiológica de residuos de ácido oxolínico y oxitetraciclina en peces, se trataron, 75 peces de la especie Salmo salar con cada uno de los antibióticos. Para ello se escogieron peces con un peso promedio de 2,7 Kg, y durante 10 días se les administró por vía oral la dosis indicada por el fabricante para ambos antibacterianos. Finalizado el período de tratamiento, se tomaron muestras de músculo y suero de 5 peces en los días 1 a 5 y 10, 15, 20 y 30 post tratamiento. Las muestras fueron conservadas en congelación para luego ser analizadas por el método de detección del antibiótico por inhibición bacteriana, utilizando Bacillus subtilis BGA y E. coli ATCC 11303 como cepas sensibles, incluidas en un medio de cultivo nutritivo a pH 6,0 y 8,0 respectivamente. Muestras de suero y de músculo de cada pez se colocaron en duplicado en cada una de las 4 placas de Petri con los diferentes substratos de cultivo. Después de la incubación de las placas a 35 ºC por 24 h se midió el halo de inhibición bacteriana (en mm provocado por la difusión del residuo. Los resultados demuestran que el ácido oxolínico y la oxitetraciclina se detectan mejor en el substrato a pH 6,0, y que la cepa Bacillus subtilis BGA, es más sensible que E.coli ATCC 11303, a la detección de ambos antibacterianos. Las muestras de suero provocaron halos de inhibición de mayor tamaño que las de músculo del mismo pez y demostraron una mayor persistencia del residuo a través del tiempo de depleción de los fármacosSimultaneously with the development of the salmoniculture, comes forth the need to use antimicrobial drugs to control fish disease thus, increasing the risk of residues in

  19. Atividades de quitinase e beta-1,3-glucanase após eliciação das defesas do tomateiro contra a mancha-bacteriana Chitinase and beta-1,3-glucanase activities after the elicitation of tomato defenses against bacterial spot

    Directory of Open Access Journals (Sweden)

    Fábio Rossi Cavalcanti

    2006-12-01

    Full Text Available O objetivo deste trabalho foi avaliar a influência de eliciadores biológicos e químicos sobre as atividades de duas proteínas relacionadas à patogênese (PR, quitinase e beta-1,3-glucanase, em folhas de tomateiro, e avaliar o potencial desses eliciadores na redução do progresso da mancha-foliar causada por Xanthomonas campestris pv. vesicatoria. Plantas de tomateiro da cultivar Santa Cruz Kada foram pulverizadas com: acibenzolar-S-metil (ASM; 0,2 g L-1; formulação biológica proveniente de biomassa cítrica, denominada Ecolife (5 mL L-1; suspensão de quitosana (MCp; 200 g L-1, proveniente de micélio de Crinipellis perniciosa; extrato aquoso de ramos de lobeira (Solanum lycocarpum infectados por C. perniciosa (VLA; 300 g L-1. As plantas foram desafiadas com um isolado virulento da bactéria, quatro dias depois das pulverizações. Plantas pulverizadas com extratos biológicos mostraram redução da mancha-bacteriana. ASM proporcionou 49,3% de proteção, e foi igual à MCp e Ecolife e superior ao VLA. Este último não diferiu significativamente de MCp e Ecolife. Observou-se maior atividade das duas enzimas nas plantas tratadas, principalmente nas primeiras horas após as pulverizações.The objective of this work was to assess the influence of foliar application of resistance inducers and the activation of plant pathogenesis-related (PR proteins, chitinases and beta-1,3-glucanases, against Xanthomonas campestris pv. vesicatoria, and evaluate the potential of these elicitors on the reduction of bacterial leaf spot. Tomato plants of the cultivar Santa Cruz Kada were sprayed with: acibenzolar-S-methyl (0.2 g L-1 ASM; Ecolife, a biological formulation based on citric biomass (5 mL L-1; chitosan suspension from Crinipellis perniciosa mycelium (MCp; 200 g L-1; an aqueous extract from branches of lobeira (Solanum lycocarpum infected with C. perniciosa (VLA; 300 g L-1. Plants were challenged with a virulent bacterial strain four days after

  20. Resistencia bacteriana a antibióticos en el Hospital San Juan de Dios, 1995-1999

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    Ricardo Boza-Cordero

    2001-07-01

    Full Text Available Justificación: La resistencia a antibióticos por parte de bacterias patógenas es un grave problema mundial. Su control ha sido difícil pero deben hacerse todos los esfuerzos por realizarlo. Conocer la epidemiología en las diferentes regiones y países se constituye en parte importante de este control. Este estudio se llevóa cabo con el fin de analizar el comportamiento de la resistencia en el hospital San Juan de Dios, centro de atención nacional de adultos de aproximadamente 700 camas. Materiales y métodos: El estudio se basóen los datos obtenidos en los años 1995-1999 ya que en 1995 se inició el empleo de equipo automatizado VITEK® en este hospital. Se analizaron 2817 aislamientos de cocos Gram positivos (CGP y 7626 de bacilos Gram negativos (BGN obtenidos de todos los sitios anatómicos y fluidos corporales. Por ser un estudio retrospectivo, no se pudo definir cuáles aislamientos provenían de infecciones intra o extrahospitalarias. Resultados: Staphylococcus aureus fue el CGP más frecuentemente aislado, mientras que el BGN más frecuente fue Escherichia coli. En más de 90% de los estafilococos aislados se demostróresistencia a la penicilina. La resistencia a la oxacilina en S.aureus se incrementóde 35% en 1995 a 52% en 1999 mientras que en los estafilococos coagulasa negativa, pasóde 70 a 77% en el mismo periodo. La resistencia a la cefalotina en S.aureus pasóde 35 a 50% mientras que en los estafilococos coagulasa negativa pasóde 65 a 76%. La resistencia a la clindamicina en ambos grupos bacterianos se mantuvo relativamente estable. No se demostróresistencia a la vancomicina. La mayoría de los enterococos aislados correspondieron a E. faecalis, 75% de ellos fueron sensibles a la penicilina y 50% no mostraron sinergismo de la gentamicina con los antibióticos que actúan sobre la pared. No se encontraron enterococos vancomicina resistentes. En las enterobacterias se observó un aumento importante de la resistencia a

  1. Sociedad por Acciones Simplificada

    Directory of Open Access Journals (Sweden)

    Carlos Andrés Arcila Salazar

    2009-07-01

    Full Text Available En Colombia, aspectos como la responsabilidad limitada; supresión de la llamada doctrina de ultra vires; duración ilimitada; eliminación de la regla, una acción, un voto; remedios al abuso de los derechos; posibilidad de eliminar prohibiciones a los administradores, libertad de establecer restricciones para la transferencia de acciones y la simplificación de los procedimientos de fusión; son inviables bajo nuestra legislación. Sin embargo, la Ley 1258 de 2008, ha incorporado al derecho societario colombiano un nuevo tipo societario híbrido, llamado “Sociedad por Acciones Simplificada”. Esta forma societaria híbrida se basa en un principio básico: garantizar amplia libertad a los empresarios en la estructuración de sus relaciones a través de disposiciones contractuales. La S.A.S. provee a los empresarios significativa flexibilidad contractual, a la vez que preserva beneficios como la limitación de la responsabilidad.

  2. Sociedad por Acciones Simplificada

    Directory of Open Access Journals (Sweden)

    Carlos Andrés Arcila Salazar

    2009-06-01

    Full Text Available En Colombia, aspectos como la responsabilidad limitada; supresión de la llamada doctrina de ultra vires; duración ilimitada; eliminación de la regla, una acción, un voto; remedios al abuso de los derechos; posibilidad de eliminar prohibiciones a los administradores, libertad de establecer restricciones para la transferencia de acciones y la simplificación de los procedimientos de fusión; son inviables bajo nuestra legislación. Sin embargo, la Ley 1258 de 2008, ha incorporado al derecho societario colombiano un nuevo tipo societario híbrido, llamado “Sociedad por Acciones Simplificada”. Esta forma societaria híbrida se basa en un principio básico: garantizar amplia libertad a los empresarios en la estructuración de sus relaciones a través de disposiciones contractuales. La S.A.S. provee a los empresarios significativa flexibilidad contractual, a la vez que preserva beneficios como la limitación de la responsabilidad.

  3. Genetically engineered Lactococcus lactis protect against house dust mite allergy in a BALB/c mouse model.

    Directory of Open Access Journals (Sweden)

    Chunqing Ai

    Full Text Available Mucosal vaccine based on lactic acid bacteria is an attractive concept for the prevention and treatment of allergic diseases, but their mechanisms of action in vivo are poorly understood. Therefore, we sought to investigate how recombinant major dust mite allergen Der p2-expressing Lactococcus lactis as a mucosal vaccine induced the immune tolerance against house dust mite allergy in a mouse model.Three strains of recombinant L. lactis producing Der p2 in different cell components (extracellular, intracellular and cell wall were firstly constructed. Their prophylactic potential was evaluated in a Der p2-sensitised mouse model, and immunomodulation properties at the cellular level were determined by measuring cytokine production in vitro.Der p2 expressed in the different recombinant L. lactis strains was recognized by a polyclonal anti-Der p2 antibody. Oral treatment with the recombinant L. lactis prior sensitization significantly prevented the development of airway inflammation in the Der p2-sensitized mice, as determined by the attenuation of inflammatory cells infiltration in the lung tissues and decrease of Th2 cytokines IL-4 and IL-5 levels in bronchoalveolar lavage. In addition, the serum allergen-specific IgE levels were significantly reduced, and the levels of IL-4 in the spleen and mesenteric lymph nodes cell cultures were also markedly decreased upon allergen stimulation in the mice fed with the recombinant L. lactis strains. These protective effects correlated with a significant up-regulation of regulatory T cells in the mesenteric lymph nodes.Oral pretreatment with live recombinant L. lactis prevented the development of allergen-induced airway inflammation primarily by the induction of specific mucosal immune tolerance.

  4. Reduced binding of the endolysin LysTP712 to Lactococcus lactis ΔftsH contributes to phage resistance

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    CLARA eROCES

    2016-02-01

    Full Text Available Absence of the membrane protease FtsH in Lactococcus lactis hinders release of the bacteriophage TP712. In this work we have analysed the mechanism responsible for the non-lytic phenotype of L. lactis ΔftsH after phage infection. The lytic cassette of TP712 contains a putative antiholin-pinholin system and a modular endolysin (LysTP712. Inducible expression of the holin gene demonstrated the presence of a dual start motif which is functional in both wildtype and L. lactis ΔftsH cells. Moreover, simulating holin activity with ionophores accelerated lysis of wildtype cells but not L. lactis ΔftsH cells, suggesting inhibition of the endolysin rather than a role of FtsH in holin activation. However, zymograms revealed the synthesis of an active endolysin in both wildtype and L. lactis ΔftsH TP712 lysogens. A reporter protein was generated by fusing the cell wall binding domain of LysTP712 to the fluorescent mCherry protein. Binding of this reporter protein took place at the septa of both wildtype and L. lactis ΔftsH cells as shown by fluorescence microscopy. Nonetheless, fluorescence spectroscopy demonstrated that mutant cells bound 40 % less protein. In conclusion, the non-lytic phenotype of L. lactis ΔftsH is not due to direct action of the FtsH protease on the phage lytic proteins but rather to a putative function of FtsH in modulating the architecture of the L. lactis cell envelope that contributes to a lower affinity of the phage endolysin to its substrate and, likely to its catalytic activity.

  5. Reduced Binding of the Endolysin LysTP712 to Lactococcus lactis ΔftsH Contributes to Phage Resistance.

    Science.gov (United States)

    Roces, Clara; Campelo, Ana B; Escobedo, Susana; Wegmann, Udo; García, Pilar; Rodríguez, Ana; Martínez, Beatriz

    2016-01-01

    Absence of the membrane protease FtsH in Lactococcus lactis hinders release of the bacteriophage TP712. In this work we have analyzed the mechanism responsible for the non-lytic phenotype of L. lactis ΔftsH after phage infection. The lytic cassette of TP712 contains a putative antiholin-pinholin system and a modular endolysin (LysTP712). Inducible expression of the holin gene demonstrated the presence of a dual start motif which is functional in both wildtype and L. lactis ΔftsH cells. Moreover, simulating holin activity with ionophores accelerated lysis of wildtype cells but not L. lactis ΔftsH cells, suggesting inhibition of the endolysin rather than a role of FtsH in holin activation. However, zymograms revealed the synthesis of an active endolysin in both wildtype and L. lactis ΔftsH TP712 lysogens. A reporter protein was generated by fusing the cell wall binding domain of LysTP712 to the fluorescent mCherry protein. Binding of this reporter protein took place at the septa of both wildtype and L. lactis ΔftsH cells as shown by fluorescence microscopy. Nonetheless, fluorescence spectroscopy demonstrated that mutant cells bound 40% less protein. In conclusion, the non-lytic phenotype of L. lactis ΔftsH is not due to direct action of the FtsH protease on the phage lytic proteins but rather to a putative function of FtsH in modulating the architecture of the L. lactis cell envelope that results in a lower affinity of the phage endolysin to its substrate. PMID:26904011

  6. Improved acid stress survival of Lactococcus lactis expressing the histidine decarboxylation pathway of Streptococcus thermophilus CHCC1524.

    Science.gov (United States)

    Trip, Hein; Mulder, Niels L; Lolkema, Juke S

    2012-03-30

    Degradative amino acid decarboxylation pathways in bacteria generate secondary metabolic energy and provide resistance against acid stress. The histidine decarboxylation pathway of Streptococcus thermophilus CHCC1524 was functionally expressed in the heterologous host Lactococcus lactis NZ9000, and the benefits of the newly acquired pathway for the host were analyzed. During growth in M17 medium in the pH range of 5-6.5, a small positive effect was observed on the biomass yield in batch culture, whereas no growth rate enhancement was evident. In contrast, a strong benefit for the engineered L. lactis strain was observed in acid stress survival. In the presence of histidine, the pathway enabled cells to survive at pH values as low as 3 for at least 2 h, conditions under which the host cells were rapidly dying. The flux through the histidine decarboxylation pathway in cells grown at physiological pH was under strict control of the electrochemical proton gradient (pmf) across the membrane. Ionophores that dissipated the membrane potential (ΔΨ) and/or the pH gradient (ΔpH) strongly increased the flux, whereas the presence of glucose almost completely inhibited the flux. Control of the pmf over the flux was exerted by both ΔΨ and ΔpH and was distributed over the transporter HdcP and the decarboxylase HdcA. The control allowed for a synergistic effect between the histidine decarboxylation and glycolytic pathways in acid stress survival. In a narrow pH range around 2.5 the synergism resulted in a 10-fold higher survival rate. PMID:22351775

  7. Production of Fibronectin Binding Protein A at the surface of Lactococcus lactis increases plasmid transfer in vitro and in vivo.

    Directory of Open Access Journals (Sweden)

    Daniela Pontes

    Full Text Available Lactococci are noninvasive lactic acid bacteria frequently used as protein delivery vectors and, more recently, as DNA delivery vehicles. We previously showed that Lactococcus lactis (LL expressing the Fibronectin-Binding Protein A of Staphylococcus aureus (LL-FnBPA+ showed higher internalization rates in vitro in Caco-2 cells than the native (wt lactococci and were able to deliver a eukaryotic Green Fluorescent Protein (GFP expression plasmid in 1% of human Caco-2 cells. Here, using the bovine beta-lactoglobulin (BLG, one of the major cow's milk allergen, and GFP we characterized the potential of LL-FnBPA+ as an in vivo DNA vaccine delivery vehicle. We first showed that the invasive strain LL-FnBPA+ carrying the plasmid pValac:BLG (LL-FnBPA+ BLG was more invasive than LL-BLG and showed the same invasivity as LL-FnBPA+. Then we demonstrated that the Caco-2 cells, co-incubated with LL-FnBPA+ BLG produced up to 30 times more BLG than the Caco-2 cells co-incubated with the non invasive LL-BLG. Using two different gene reporters, BLG and GFP, and two different methods of detection, EIA and fluorescence microscopy, we showed in vivo that: i in order to be effective, LL-FnBPA+ required a pre-coating with Fetal Calf Serum before oral administration; ii plasmid transfer occurred in enterocytes without regard to the strains used (invasive or not; iii the use of LL-FnBPA+ increased the number of mice producing BLG, but not the level of BLG produced. We thus confirmed the good potential of invasive recombinant lactic acid bacteria as DNA delivery vector in vivo.

  8. Reduced Binding of the Endolysin LysTP712 to Lactococcus lactis ΔftsH Contributes to Phage Resistance

    Science.gov (United States)

    Roces, Clara; Campelo, Ana B.; Escobedo, Susana; Wegmann, Udo; García, Pilar; Rodríguez, Ana; Martínez, Beatriz

    2016-01-01

    Absence of the membrane protease FtsH in Lactococcus lactis hinders release of the bacteriophage TP712. In this work we have analyzed the mechanism responsible for the non-lytic phenotype of L. lactis ΔftsH after phage infection. The lytic cassette of TP712 contains a putative antiholin–pinholin system and a modular endolysin (LysTP712). Inducible expression of the holin gene demonstrated the presence of a dual start motif which is functional in both wildtype and L. lactis ΔftsH cells. Moreover, simulating holin activity with ionophores accelerated lysis of wildtype cells but not L. lactis ΔftsH cells, suggesting inhibition of the endolysin rather than a role of FtsH in holin activation. However, zymograms revealed the synthesis of an active endolysin in both wildtype and L. lactis ΔftsH TP712 lysogens. A reporter protein was generated by fusing the cell wall binding domain of LysTP712 to the fluorescent mCherry protein. Binding of this reporter protein took place at the septa of both wildtype and L. lactis ΔftsH cells as shown by fluorescence microscopy. Nonetheless, fluorescence spectroscopy demonstrated that mutant cells bound 40% less protein. In conclusion, the non-lytic phenotype of L. lactis ΔftsH is not due to direct action of the FtsH protease on the phage lytic proteins but rather to a putative function of FtsH in modulating the architecture of the L. lactis cell envelope that results in a lower affinity of the phage endolysin to its substrate. PMID:26904011

  9. Complete sequences of four plasmids of Lactococcus lactis subsp. cremoris SK11 reveal extensive adaptation to the dairy environment.

    Science.gov (United States)

    Siezen, Roland J; Renckens, Bernadet; van Swam, Iris; Peters, Sander; van Kranenburg, Richard; Kleerebezem, Michiel; de Vos, Willem M

    2005-12-01

    Lactococcus lactis strains are known to carry plasmids encoding industrially important traits. L. lactis subsp. cremoris SK11 is widely used by the dairy industry in cheese making. Its complete plasmid complement was sequenced and found to contain the plasmids pSK11A (10,372 bp), pSK11B (13,332 bp), pSK11L (47,165 bp), and pSK11P (75,814 bp). Six highly homologous repB-containing replicons were found, all belonging to the family of lactococcal theta-type replicons. Twenty-three complete insertion sequence elements segment the plasmids into numerous modules, many of which can be identified as functional units or containing functionally related genes. Plasmid-encoded functions previously known to reside on L. lactis SK11 plasmids were now mapped in detail, e.g., lactose utilization (lacR-lacABCDFEGX), the proteolytic system (prtM-prtP, pepO, pepF), and the oligopeptide permease system (oppDFBCA). Newly identified plasmid-encoded functions could facilitate the uptake of various cations, while the pabA and pabB genes could be essential for folate biosynthesis. A competitive advantage could be obtained by using the putative flavin adenine dinucleotide-dependent d-lactate dehydrogenase and oxalate:formate antiporter for enhanced ATP synthesis, while the activity of the predicted alpha-acetolactate decarboxylase may contribute to the formation of an additional electron sink. Various stress response proteins are plasmid encoded, which could enhance strain robustness. A substantial number of these "adaptation" genes have not been described before on L. lactis plasmids. Moreover, several genes were identified for the first time in L. lactis, possibly reflecting horizontal gene transfer. PMID:16332824

  10. Mutations in the Lactococcus lactis Ll.LtrB group II intron that retain mobility in vivo

    Directory of Open Access Journals (Sweden)

    D'Souza Lisa M

    2002-12-01

    Full Text Available Abstract Background Group II introns are mobile genetic elements that form conserved secondary and tertiary structures. In order to determine which of the conserved structural elements are required for mobility, a series of domain and sub-domain deletions were made in the Lactococcus lactis group II intron (Ll.LtrB and tested for mobility in a genetic assay. Point mutations in domains V and VI were also tested. Results The largest deletion that could be made without severely compromising mobility was 158 nucleotides in DIVb(1–2. This mutant had a mobility frequency comparable to the wild-type Ll.LtrB intron (ΔORF construct. Hence, all subsequent mutations were done in this mutant background. Deletion of DIIb reduced mobility to approximately 18% of wild-type, while another deletion in domain II (nts 404–459 was mobile to a minor extent. Only two deletions in DI and none in DIII were tolerated. Some mobility was also observed for a DIVa deletion mutant. Of the three point mutants at position G3 in DV, only G3A retained mobility. In DVI, deletion of the branch-point nucleotide abolished mobility, but the presence of any nucleotide at the branch-point position restored mobility to some extent. Conclusions The smallest intron capable of efficient retrohoming was 725 nucleotides, comprising the DIVb(1–2 and DII(iia,b deletions. The tertiary elements found to be nonessential for mobility were alpha, kappa and eta. In DV, only the G3A mutant was mobile. A branch-point residue is required for intron mobility.

  11. Transcriptome analysis of Lactococcus lactis subsp. lactis during milk acidification as affected by dissolved oxygen and the redox potential.

    Science.gov (United States)

    Larsen, Nadja; Moslehi-Jenabian, Saloomeh; Werner, Birgit Brøsted; Jensen, Maiken Lund; Garrigues, Christel; Vogensen, Finn Kvist; Jespersen, Lene

    2016-06-01

    Performance of Lactococcus lactis as a starter culture in dairy fermentations depends on the levels of dissolved oxygen and the redox state of milk. In this study the microarray analysis was used to investigate the global gene expression of L. lactis subsp. lactis DSM20481(T) during milk acidification as affected by oxygen depletion and the decrease of redox potential. Fermentations were carried out at different initial levels of dissolved oxygen (dO2) obtained by milk sparging with oxygen (high dO2, 63%) or nitrogen (low dO2, 6%). Bacterial exposure to high initial oxygen resulted in overexpression of genes involved in detoxification of reactive oxygen species (ROS), oxidation-reduction processes, biosynthesis of trehalose and down-regulation of genes involved in purine nucleotide biosynthesis, indicating that several factors, among them trehalose and GTP, were implicated in bacterial adaptation to oxidative stress. Generally, transcriptional changes were more pronounced during fermentation of oxygen sparged milk. Genes up-regulated in response to oxygen depletion were implicated in biosynthesis and transport of pyrimidine nucleotides, branched chain amino acids and in arginine catabolic pathways; whereas genes involved in salvage of nucleotides and cysteine pathways were repressed. Expression pattern of genes involved in pyruvate metabolism indicated shifts towards mixed acid fermentation after oxygen depletion with production of specific end-products, depending on milk treatment. Differential expression of genes, involved in amino acid and pyruvate pathways, suggested that initial oxygen might influence the release of flavor compounds and, thereby, flavor development in dairy fermentations. The knowledge of molecular responses involved in adaptation of L. lactis to the shifts of redox state and pH during milk fermentations is important for the dairy industry to ensure better control of cheese production. PMID:27015296

  12. Bidirectional cell-surface anchoring function of C-terminal repeat region of peptidoglycan hydrolase of Lactococcus lactis IL1403.

    Science.gov (United States)

    Tarahomjoo, Shirin; Katakura, Yoshio; Satoh, Eiichi; Shioya, Suteaki

    2008-02-01

    With the aim of constructing an efficient protein display system for lactic acid bacteria (LABs), the effect of fusion direction on the cell-surface binding activity of the C-terminal region of the peptidoglycan hydrolase (CPH) of Lactococcus lactis IL1403 was studied. CPH fused to the alpha-amylase (AMY) of Streptococcus bovis 148 either at its C-terminus (CPH-AMY) or at its N-terminus (AMY-CPH) was expressed intracellularly in Escherichia coli. This domain was able to direct binding of AMY to the surface of L. lactis ATCC 19435 in both constructs. However, the number of bound molecules per cell and the specific activity for starch digestion in the case of CPH-AMY were 3 and 14 times greater than those in the case of AMY-CPH, respectively. Of the LABs tested, L. lactis ATCC 19435 showed the highest binding capability for CPH-AMY, up to 6 x 10(4) molecules per cell, with a dissociation rate constant of 5.00 x 10(-5) s(-1). The binding of CPH-AMY to the surface of Lactobacillus delbrueckii ATCC 9649 cells was very stable with a dissociation rate constant of 6.96 x 10(-6) s(-1). The production of CPH-AMY in the soluble form increased 3-fold as a result of coexpression with a molecular chaperone, trigger factor. The results of this study suggest the usefulness of CPH as a bidirectional anchor protein for the production of cell-surface adhesive enzymes in E. coli. Furthermore, the importance of the fusion direction of CPH in determining cell-surface binding and enzymatic activities was shown. PMID:18343337

  13. Infecção por Salmonella typhimurium de origem hídrica em garça gigante (Casmerodius albus egretta, em sua vida livre no Estado do Rio de Janeiro

    Directory of Open Access Journals (Sweden)

    Maria do Amparo Queiroz de Freitas

    1977-10-01

    Full Text Available De uma mortandade de garças de vida livre, no Estado do Rio de Janeiro, Brasil, foi isolada Salmonella typhimurium por hemocultura de material proveniente de uma ave que se mostrava enferma e que à necrópsia não apresentavam lesões a na tomopa tológicas. A Salmonella typhimurium foi também isolada de água de um lago existente no Jardim Zoológico do Rio de Janeiro, onde as aves tinham acesso permanente. Durante a mortandade das aves foi observada uma hepatite necrótica na qual havia colônias bacterianas, tesões atribuídas a esta salmonelose.

  14. 乳球菌介导的重组细菌疫苗的研制现状%Current condition of researches in recombinant bacteria mediated by lactococcus

    Institute of Scientific and Technical Information of China (English)

    李文桂; 陈雅棠

    2016-01-01

    细菌是一种人类感染的常见病原体,研制疫苗防治细菌感染是当前研究的热点领域之一。乳球菌是一种新型疫苗载体,本文阐述了乳球菌介导的肺炎链球菌、化脓链球菌、金黄色葡萄球菌、大肠埃希菌、奇异变形杆菌、流产布鲁菌、鼠疫耶尔森菌、破伤风梭菌、单增李斯特菌、霍乱弧菌、幽门螺杆菌、铜绿假单胞菌和嗜水气单胞菌等细菌疫苗的构建及其作用机制等方面的研制现状。%Bacteria is a common type of human infectious pathogen agent ,whose prevention measure ,vaccine , has become a popular research topic during recent years .Lactococcus is a new type of vaccine carrier .This article reviews the present condition of researches in construction and immune mechanisms of recombinant vaccine of bacteria ,such as Streptococcus pneumoniae ,Streptococcus pyogenes ,Staphylococcus aureus ,Escherichia coli ,Proteus mirabilis ,Brucella abortus ,Yersinia pestis ,Clostridium tetanus ,Listeria monocytogenes ,Vibro cholera ,Helicobacter pylori ,Pseudomonas aeruginosa and Aeromonas hydrophila mediated by lactococcus .

  15. Temperate phages TP901-1 and fLC3, belonging to the P335 species, apparently use different pathways for DNA injection in Lactococcus lactis subsp. cremoris 3107

    DEFF Research Database (Denmark)

    Breum, Solvej Østergaard; Neve, Horst; Heller, Knut J.; Vogensen, Finn Kvist

    2007-01-01

    Five mutant of Lactococcus lactis subsp. cremoris 3107 resistant to phage TP901-1 were obtained after treatment with ethyl methanesulfonate. Two of the mutants were also resistant to phage fLC3. The remaining three mutants were as sensitive as 3107. Mutants E46 and E100 did not adsorb the two pha...

  16. Proteome analysis of a Lactococcus lactis strain overexpressing gapA suggests that the gene product is an auxiliary glyceraldehyde 3-phosphate dehydrogenase

    DEFF Research Database (Denmark)

    Willemoës, Martin; Kilstrup, Mogens; Roepstorff, Peter;

    2002-01-01

    The sequence of the genome from the Lactococcus lactis subspecies lactis strain IL1403 shows the presence of two reading frames, gapA and gapB, putatively encoding glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Previous proteomic analysis of the L. lactis subspecies cremoris strain MG1363 has ...

  17. Probióticos em rações para frangos de corte utilizando farinha de carne e ossos com diferentes níveis de contaminação bacteriana Use of probiotics in broiler rations containing bone and meat meal with different levels of bacterial contamination

    Directory of Open Access Journals (Sweden)

    Antônio Soares Teixeira

    2003-08-01

    Full Text Available Com o objetivo de estudar os efeitos de probióticos no desempenho de frangos de corte consumindo dietas com farinhas de carne e ossos com diferentes contaminações bacterianas, foi realizado um experimento na Universidade Federal de Lavras, no qual foram utilizados 576 pintos da linhagem Hubbard, com um dia de idade, metade de cada sexo. O delineamento experimental foi inteiramente casualizado, num esquema fatorial 4 x 3 (rações e probióticos com 4 repetições de 12 aves alojadas em baterias metálicas e o período experimental foi de 1 a 28 dias de idade. Os tratamentos foram constituídos de 4 rações, uma sem farinha de carne e ossos e com fosfato bicálcico (testemunha, e as outras três com farinhas de carne e ossos de alto (1,0 x 104, médio(4,5 x 103 e baixo (1,0 x 103 teor de contaminação bacteriana. Cada uma dessas rações foi fornecida sem probiótico, ou com um probiótico com cerca de três bilhões de células viáveis por grama, que tem como agentes ativos o Lactobacillus acidophilus, Streptococcus faecium e Sacharomices cerevisae, fornecido na ração na dose de 10 g/ton ou ainda com um probiótico que contém Enterococcus faecium, Lactobacillus acidófillus, Lactobacillus plantarum e Lactobacillus sp como agentes ativos, na concentração de 3 x 1010 ufc/g do produto, adicionado na água nas primeiras 24 horas de vida dos pintinhos, na proporção de 1,33 g/L de água. Observou-se pela análise da variância dos resultados que não houve efeito dos tratamentos (P>0,05 sobre o ganho de peso e consumo de ração e na conversão alimentar, para as aves que consumiram a ração com baixa contaminação. A inclusão de farinha de carne e ossos nas rações melhorou a conversão alimentar para as aves que receberam a ração com alta e média contaminação, independente do uso de probiótico. Verificaram-se redução nos casos de diarréia durante a primeira semana de vida dos pintinhos, com o uso de probióticos, e aumento

  18. Translocação bacteriana para o rim na icterícia obstrutiva experimental Bacterial translocation to kidney in obstructive experimental jaundice

    Directory of Open Access Journals (Sweden)

    Aldo da Cunha Medeiros

    1997-06-01

    Full Text Available Estudo com o objetivo de verificar a possibilidade de ocorrência de translocação bacteriana para os rins de ratos, após ligadura de colédoco. Foram utilizados 29 ratos Wistar com peso médio de 171 ± 12,6g. Os animais foram divididos aleatoriamente em grupo I (n=14 e grupo II (n=15. Com técnica asséptica, nos ratos no grupo I foi feita ligadura de colédoco com fio de seda nº 3 zeros e no grupo II foi simulada a ligadura com a simples manipulação do colédoco com pinça de Adson ("sham operation". No 7º dia pós-operatório os animais foram sacrificados e ressecados os rins para exame histopatológico (coloração H.E. e microbiológico (meios agar sangue e agar MacConkey. Houve crescimento bacteriano de Klebsiela sp em 28,5% dos casos no grupo I e foram observadas alterações histopatológicas significativas no mesmo grupo. As diferenças foram estatisticamente significantes quando foram comparados os 2 grupos (pExperimental study in order to determine if obstructive jaundice promotes basterial translocation from the gastrointestinal tract to the kidney in rats. We used 29 Wistar male rats weighing 171±12,6g. They were ramdomly separated in goup I (n=14 and group II (n=15 and operated with asseptic technic. The group I underwent common bile ducts ligature with number 000 silk suture, and in group II (control the common bile ducts were manipulated with Adson forceps, as a sham operation. On 7th postoperative day the rats were killed with an overdose of anesthesic and had their kidneys resected. Portions of each kidney were harvested for quantitation of bacterial translocation and histopatologic examination. The incidance of bacterial translocation was 28,5% (Klebsiella sp in group I and 0% in group II (p<0,05. Intersticial edema, nephrosis and tubular degeneration were observed in group I and the histology was normal in group II. The authors conclude that obstructive ajundice promotes bacterial translocation to the kidney

  19. 重组乳酸乳球菌表达外源产物在养猪生产中的潜在应用%Potential Application of Expression of Exogenous Products by Recombinant Lactococcus lactis in Pig Production

    Institute of Scientific and Technical Information of China (English)

    张攀; 许蒙蒙; 林燕; 方正锋; 车炼强; 吴德; 徐盛玉

    2015-01-01

    Lactococcus lactis is a typical representative bacterium of lactic acid bacteria. With the deepening re-search in molecular biology of Lactococcus lactis, recombinant Lactococcus lactis is extensively researched as a bacterial carrier in the field of animal husbandry and veterinary due to having prebiotic effects and using expres-sion of exogenous functional proteins. Meanwhile, Lactococcus lactis also showed good potential in pig indus-try, which could provide a new way for the healthy development of pig industry. This paper reviewed the ex-pression of exogenous products of recombinant Lactococcus lactis ( epidermal growth factor, lactoferrin, etc. ) for improving pig performance as well as a vaccine carrier for prevention and treatment of swine diseases.%乳酸乳球菌是乳酸菌中的典型代表,随着乳酸乳球菌分子生物学研究的不断深入,重组乳酸乳球菌因具有益生作用和表达外源功能蛋白的双重功能被用做载体菌在畜牧兽医学领域广泛研究,同时在养猪产业中也显示出良好的应用潜力,可为养猪业的健康发展提供新的思路. 本文主要对重组乳酸乳球菌表达表皮生长因子、乳铁蛋白等外源产物来提高猪生产性能以及作为疫苗呈递载体用于猪疾病防治的应用做一综述.

  20. Bacterial diversity of soil under eucalyptus assessed by 16S rDNA sequencing analysis Diversidade bacteriana de solo sob eucaliptos obtida por seqüenciamento do 16S rDNA

    OpenAIRE

    Érico Leandro da Silveira; Rodrigo Matheus Pereira; Denilson César Scaquitto; Eliamar Aparecida Nascimbém Pedrinho; Silvana Pómpeia Val-Moraes; Ester Wickert; Lúcia Maria Carareto-Alves; Eliana Gertrudes Macedo Lemos

    2006-01-01

    Studies on the impact of Eucalyptus spp. on Brazilian soils have focused on soil chemical properties and isolating interesting microbial organisms. Few studies have focused on microbial diversity and ecology in Brazil due to limited coverage of traditional cultivation and isolation methods. Molecular microbial ecology methods based on PCR amplified 16S rDNA have enriched the knowledge of soils microbial biodiversity. The objective of this work was to compare and estimate the bacterial diversi...

  1. Stability of active prophages in industrial Lactococcus lactis strains in the presence of heat, acid, osmotic, oxidative and antibiotic stressors.

    Science.gov (United States)

    Ho, Chun-Hoong; Stanton-Cook, Mitchell; Beatson, Scott A; Bansal, Nidhi; Turner, Mark S

    2016-03-01

    Lactococcus lactis is a starter bacterium commonly used in cheese making where it has an important role in acid-mediated curd formation as well as the development of flavour compounds. Industrial L. lactis strains can harbour one or more inducible prophages which when induced can affect cell growth and possibly lead to cell lysis. This is undesirable during growth and fermentation, but can beneficially lead to faster release of enzymes during cheese ripening. Lactococci can encounter multiple stress inducing conditions during the production of cheese, such as low and high temperatures, low pH, high osmotic pressure and long-term incubation. In this study, we tested the effect of these industrial stressors on prophage induction in two cheese making L. lactis subsp. cremoris strains (ASCC890049 and ASCC890310) as well as the laboratory strain L. lactis MG1363. Firstly, in order to identify inducible prophages in these strains we exposed them to the prophage inducing chemical mitomycin C (MMC) for 1 and 2h and then subjected the total genomic DNA to next-generation Illumina sequencing. Mapping of sequence reads back to the genome sequences revealed regions which contained a much higher fold coverage indicating DNA replication. These regions were amplified by up to 332-fold per cell (relative to the control tufA gene) and were identified as having similarities to different subgroups of P335 phages including MG-5, TP901-1, ul36.k1, bIL286, TP712 and BK5-T. Next, quantitative PCR was used to confirm the strong induction of prophages by MMC and then determine the copy number of the inducible prophages following exposure to various growth inhibitory levels of HCl, lactic acid, high temperature, NaCl, hydrogen peroxide and bacitracin. With the exception of a slight induction (2 to 4-fold) with hydrogen peroxide and long-term incubation after 21days in one industrial strain, none of the other stressors induced prophage DNA replication. These findings show that the repression

  2. A derivative of Lactococcus lactis strain H61 with less interleukin-12 induction has a different cell wall.

    Science.gov (United States)

    Kimoto-Nira, H; Suzuki, C; Aoki, R; Kobayashi, M; Mizumachi, K

    2012-06-01

    Lactococcus lactis H61 can increase the cellular immune responses of aged (14-mo-old) senescence-accelerated mice. The aim of this study was to investigate the factors contributing to IL-12 induction by strain H61 by analyzing strains derived from it. Strain H61 derivative no. 13 was obtained by growing the parent strain at 37°C. This derivative induced significantly lower production of IL-12 from J774.1 macrophage cells than did the parent strain H61. The 2 strains differed in the resistance of their whole cells or cell walls to lysozyme, a cell wall-degrading enzyme. Sodium hydroxide treatment to de-O-acetylate muramic acid in the cell walls of the 2 strains reduced the lysozyme resistance, compared with untreated cell walls: at 3h after adding lysozyme, the lysozyme resistance of untreated and NaOH treated cell wall from strain H61 was 55.4% and 11.7%, respectively. The values of untreated and NaOH-treated cell walls from strain no.13 were 73.7 and 42.8%, respectively. The reduction was higher in strain H61, indicating that the cell walls of strain H61 were highly O-acetylated. Trichloroacetic acid treatment to remove wall-associated polymers such as teichoic acids made the lysozyme resistance of the cell walls of both strains similar. The sugar content of cell walls prepared from strain H61 was significantly higher than that of strain no. 13 cell wall. A derivative with less activity for inducing IL-12 by macrophage cells had less O-acetylation and had lower sugar content in the cell wall than did strain H61. Modifying the cell wall of strain H61 may be a useful way to regulate its ability to induce IL-12. Strain H61 has been used as a starter bacterium in the dairy industry. This study could lead to enhancing the value of dairy products made by strain H61 by characterizing the key factor(s) responsible for its stimulation of immunity. PMID:22612923

  3. Genotypic and phenotypic diversity of Lactococcus lactis isolates from Batzos, a Greek PDO raw goat milk cheese.

    Science.gov (United States)

    Psoni, L; Kotzamanidis, C; Yiangou, M; Tzanetakis, N; Litopoulou-Tzanetaki, E

    2007-03-10

    The genotypic and phenotypic variability of 40 Lactococcus lactis isolates obtained from three cheese-making trials of Batzos cheese made one in each, winter, spring and summer was investigated. RAPD-PCR, plasmid profiling and PFGE were used to study the genetic variability and distinguish closely related isolates. Results showed a high degree of heterogeneity among strains. According to PFGE data, all strains except one were clustered together (at a similarity level of approximately 50%) with the L. lactis subsp. lactis reference strain and eleven groups of isolates consisting of 2-8 strains each were distinguished. Plasmid profiling results revealed that there were eight isolates lacking plasmids and nine having unique plasmids. Twenty-three isolates were allocated into six groups. There was an interesting similarity between the plasmid profiling groups and those formed according to PFGE. Clustering of strains according to RAPD-PCR was in agreement with results obtained by both plasmid profiling and PFGE for the majority of the strains. In addition, results obtained by molecular methods indicate a grouping of most of the strains according to the season of cheese production. All strains inhibited the growth of Escherichia coli O157:H7. Their ability to affect the growth of Yersinia enterocolitica, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes and Enterococcus faecalis was strain dependent. In 42.5% of the isolates high acidifying ability in milk after 24 h was recorded and these were isolates, mainly, from fresh cheese. The 75% of the isolates from winter cheese exhibited higher Lys- than Leu-aminopeptidase activity while the approximately 67% of the isolates from summer cheese showed higher Leu- than Lys-aminopeptidase activity. Their caseinolytic activity after growth in milk for 24 h was significant with preference for alpha(s)-casein degradation. The majority (90%) of the strains formed methanethiol from methionine and this ability was strain

  4. Obtención de microcapsulas de tamaño controlado conteniendo Lactococcus lactis, utilizando mezclas de gelanos de alto y bajo acilo

    Directory of Open Access Journals (Sweden)

    Rafael Emilio Gonzalez

    2013-12-01

    Full Text Available Título en ingles: Obtaining size-controlled microcapsules by ionic gelation with high and low acyl gellans containing Lactococcus lactisResumen: Control the diameter of microcapsules obtained with functional biopolymer is a crucial parameter in the success of food applications, since it affects the protection of microencapsulated microorganism and also in the texture of the final product. The aim of this study was to assess the obtaining of controlled size microcapsules containing Lactococcus lactis, using mixtures of high acyl gellan (HA and low acyl gellan (LA. A concentration of 0.2% (w/w gellan was employed using a simple design, generating the following mixtures: 100HA/0.0LA, 0.0HA/100LA, 25HA/75LA, 50HA/50LA and 75HA/25LA. The diameter of the microcapsules, efficiency of microencapsulation and viability of the microencapsulated microorganism were studied in function of the speed of agitation (400-800 rpm and surfactant concentration (sorbitan monooleate (0.0-0.2%v/v. The results indicated that mixtures with concentration equal or greater than 50% of HA gellan are not efficient for obtaining microcapsules, only the LA gellan and the mixture 25HA/75LA gave acceptable results. The viability of the microorganism and the efficiency of microencapsulation were descending function of the stirring speed and surfactant concentration. The microcapsules obtained had diameters not greater than 80 µm when the highest concentrations of surfactant (0.2% v/v and stirring speed (800 rpm were used, suggesting that the ionic gelation can be used to obtain microcapsules of controlled size (15-75 µm containing Lactococcus lactis with high viability (83.32% and high efficiency of microencapsulation (82.4%, which makes it feasible for use in food applications.Palabras clave: microencapsulation, high acyl gellan, low acyl gellan, ionic gelation, Lactococcus lactisSummary: Controlar el diámetro de microcápsulas obtenidas con biopolímeros funcionales es un

  5. Inativação bacteriana e sensorialidade em bebidas formuladas a partir de extrato reconstituído de Ocimum gratissimum L. (Alfavaca - Labiatae - (Lamiaceae Bacterial inactivation and sensory analysis in drink formulations prepared with Ocimum gratissimum L. ("African basil" - Labiatae - (Lamiaceae reconstituted extract

    Directory of Open Access Journals (Sweden)

    Marcelo Gonzalez Passos

    2010-06-01

    Full Text Available A partir da formulação de quatro bebidas, duas alcoólicas e duas não alcoólicas, com e sem açúcar, respectivamente, e do extrato reconstituído (alcoolatura/planta verde de Ocimum gratisimum L. ("alfavacão", "alfavaca", "alfavaca-cravo" - Labiatae - (Lamiaceae em diferentes concentrações (5, 15 e 30%, foi determinada, através de testes de suspensão em sistema de tubos múltiplos, a intensidade de atividade de inativação bacteriana (IINAB/bactericidia, sobre Salmonella Enteritidis (ATCC 11076, bem como a aceitabilidade/preferência sensorial por escala hedônica destes quatro produtos. Todas as formulações apresentaram atividade bactericida para Salmonella Enteritidis, diretamente proporcional às concentrações do extrato e ao tempo de exposição da bactéria às bebidas, destacando-se, neste sentido, a formulação não alcoólica com açúcar. Na análise sensorial, a preferência aumentou com o decréscimo da concentração de extrato de Ocimum gratissimum na formulação. A bebida não alcoólica com açúcar, na concentração de 5% de extrato, destacou-se na preferência/aceitabilidade.The activity intensity of bacterial inactivation (IINAB/bactericidie on Salmonella Enteritidis (ATCC 11076 was determined in four drink formulations, two alcoholic and two no-alcoholic, with and without sugar, respectively, and in the reconstituted extract (alcoholature/green plant of Ocimum gratissimum L. (African basil - Labiatae - (Lamiaceae at different concentrations (5, 15, and 30% through suspension tests in multiple tube system. The acceptability/sensory preference of these four products was also determined using a hedonic scale. The bactericidal activity of all formulations against Salmonella Enteritidis was found to be directly proportional to the extract concentrations and the exposure time of the bacterium in the beverages, especially the non-alcoholic formulation with sugar. In the sensorial analysis, the preference increased

  6. Avaliação da resposta inflamatória traqueal ao curativo de celulose bacteriana após escarificação cirúrgica em coelhos Tracheal inflammatory response to bacterial cellulose dressing after surgical scarification in rabbits

    Directory of Open Access Journals (Sweden)

    Angelo D'urso Panerari

    2008-08-01

    Full Text Available Dentre as causas de insucesso nas cirurgias para a estenose traqueal está a formação de tecidos de cicatrização exuberantes. O uso de curativos para evitar esta reação pode ser de grande valia nestes casos. A celulose bacteriana produzida por acetobacter xylinun pode ser útil nestes casos. Não há estudos na região laringotraqueal. OBJETIVO: Avaliar a resposta tecidual subglótica de coelhos após escarificação e colocação de curativo de celulose, comparando com grupo controle. FORMA DE ESTUDO: Experimental MATERIAL E MÉTODOS: Foram estudados 26 coelhos, submetidos a escarificação da região laringotraqueal e tratados com curativo e comparados com controle. Foram estabelecidos 4 tempos de seguimento. Os seguimentos laringotraqueais foram examinados histologicamente e os resultados foram avaliados estaticamente. RESULTADOS: O grupo de estudo evoluiu com o passar do tempo com resultados estatisticamente semelhantes ao do grupo controle, nos parâmetros Congestão vascular, Exsudato purulento, Inflamação aguda, Integridade do epitélio, Proliferação fibrosa e Reação granulomatosa. CONCLUSÃO: Não foram observadas diferenças entre os grupos controle e de estudo quanto aos parâmetros inflamatórios ou cicatriciais. Não houve sinais inflamatórios relacionados ao uso da membrana de celulose que não tivessem ocorrido devido ao traumatismo cirúrgico.Exuberant scarring tissue formation is among the failure causes of tracheal stenosis surgery. Dressings that could avoid such reaction could be very helpful in these cases. Bacterial cellulose, produced by acetobacter xylinun can be useful in these cases. There are no studies in the laryngotracheal region. AIM: to assess subglottic tissue response in rabbits after scarification and placement of cellulose dressing, and comparing it to a control group. STUDY DESIGN: experimental. MATERIALS AND METHODS: 26 rabbits underwent laryngotracheal scarification, received the dressing and

  7. Erosión Bacteriana del Caparazón en el camarón Penaeus vannamei (Bacterial Erosion of the Shell in the species of shrimp Penaeus vannamei

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    Fonseca Moreno, E.

    2010-05-01

    Full Text Available ResumenEl presente trabajo describe un caso clínico de Erosión Bacteriana del Caparazón en camarones de la especie Penaeus vannamei, diagnosticado a partir de la observación macroscópica y microscópica de muestras de cutícula, exponiéndose fotografías de los resultados. También se presentan algunas consideraciones relacionadas con loselementos que frecuentemente aparecen previamente en esta enfermedad.SummaryThe present work describes e clinical case of Bacterium Erosion of the Shell in shrimps of the species Penaeus vannamei. The diagnosis was carried out by the macroscopic and microscopic observation of cuticles. It exposes photos of the results too. It shows some considerations about the elements that usually appear previous ofthis disease.

  8. DESENVOLVIMENTO DE MODELO DE PERITONITE BACTERIANA PARA AVALIAÇÃO DO TRATAMENTO MEDIANTE ACESSO LAPAROTÔMICO E VIDEO-LAPAROSCÓPICO DEVELOPMENT A MODEL OF BACTERIAL PERITONITES FOR THE EVALUATION OF THE TREATMENTS THROUGH LAPAROTOMY AND LAPAROSCOPY

    OpenAIRE

    W. Salgado Jr; Cunha FQ; A.S. Sankarankuty; JS Santos

    2001-01-01

    Introdução: O emprego do acesso videolaparoscópico no tratamento das afecções digestivas que cursam com peritonite generalizada é motivo de controvérsia. Objetivo: Desenvolver um modelo de peritonite bacteriana para avaliação do tratamento mediante acesso laparotômico e videolaparoscópico. Métodos: Ratos machos Wistar foram submetidos à ligadura de ceco (CLP) sob molde rígido de 3mm de diâmetro; na seqüência foram feitas 14 punções no ceco com agulha 15X10. Após 6 horas de indução da peritoni...

  9. Comunidade bacteriana como indicadora do efeito de feijoeiro geneticamente modificado sobre organismos não alvo Bacterial community as an indicator of genetically modified common bean effect on nontarget organisms

    Directory of Open Access Journals (Sweden)

    Adriano Moreira Knupp

    2009-12-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito do feijoeiro geneticamente modificado quanto à resistência ao Bean Golden Mosaic Vírus, BGMV (Olathe M1-4, sobre organismos não alvo. De um experimento implantado no campo, em delineamento inteiramente casualizado, com dois tratamentos (Olathe Pinto e evento elite Olathe M1-4, dois períodos amostrais (estádio V4 e R6 e dez repetições, obtiveram-se células bacterianas cultivadas e não cultivadas da rizosfera e do solo não rizosférico, para as quais se procedeu à extração de DNA total. A região V6-V8 do 16S rDNA foi amplificada para a comunidade bacteriana total, e também realizou-se amplificação com iniciadores específicos para o subgrupo alfa (α do filo Proteobacteria a partir de células não cultivadas. Foram obtidos dendrogramas comparativos entre a variedade Olathe Pinto (convencional e o evento elite Olathe M1-4 (geneticamente modificado utilizando-se o coeficiente de Jaccard e o método UPGMA (Unweighted pair-group method with arithmetic mean. Os agrupamentos obtidos dos perfis de 16S rDNA PCR-DGGE indicam alterações na comunidade bacteriana da rizosfera em função da transformação das plantas são mais notáveis nos perfis obtidos para alfa-proteobacteria. A origem das amostras e o estágio de desenvolvimento das plantas afetam a comunidade bacteriana.The objective of this work was to evaluate the effect of genetically modified common bean for Bean Golden Mosaic Virus, BGMV, resistance (Olathe M1-4 on nontarget organisms. In a field experiment established in a completely randomized design with two treatments (Olathe Pinto cultivar and M1-4 Olathe elite event, two sampling periods (V4 and R6 stages and ten replicates, cultivated and non-cultivated bacterial cells from rhizosphere soil and bulk soil were obtained, and their total DNA was extracted. The V6-V8 region of 16S rDNA was amplified for the whole bacterial community, and primers specific for the alpha (

  10. Eficacia de cinco desinfectantes para la reducción bacteriana doméstica Efficacy of five disinfectants to reduce bacterial load in the household

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    Julián Stambullian

    2011-06-01

    Full Text Available El uso adecuado de hipoclorito de sodio, sales de amonio cuaternario y triclosán ha demostrado ser eficaz para eliminar gérmenes dentro del hogar. Nuestro objetivo fue evaluar la eficacia inmediata, a la semana y al mes del uso controlado de cinco productos con estos componentes, comparados con otros productos de uso habitual. Se incluyeron 32 hogares de clase media de la Ciudad de Buenos Aires y la periferia en un estudio con intervención, abierto, aleatorizado, y de grupos paralelos, durante 6 meses. La mitad de los hogares fue seleccionada para usar hipoclorito de sodio, sales de amonio cuaternario y triclosán en la cocina y el baño durante un mes. El grupo control mantuvo prácticas habituales de limpieza doméstica. Se tomaron muestras para recuento y tipificación bacteriana de los sitios estudiados: muestras basales (sin discriminación de grupo en cocina, que presentaron recuento bacteriano promedio de 66.0 UFC/cm²; baño: 40.1 UFC/cm². Las muestras inmediatas a la limpieza (sin discriminación de grupo: en cocina: 0.8 UFC/cm²; baño: The proper use of products containing sodium hypochlorite, ammonium salts and triclosan has proved to be effective in the elimination of infectious agents in the household environment. Our objective was to evaluate the immediate, one-week and one-month efficacy of controlled use of five products containing these components, compared to other commonly used products. Within a six month period, thirty two middle-class homes from Buenos Aires City and suburbs were included in this open-label, randomized, parallel-group intervention study. Sixteen homes were randomized to use products containing sodium hypochlorite, ammonia and triclosan in the kitchen and bathroom during one month. The remaining maintained usual practices for domestic cleaning. Bacterial counts and identification were performed from samples taken from each study site. Baseline samples (no group discrimination contained a mean bacterial

  11. Bacterial diversity in soil in response to different plans, phosphate fertilizers and liming Diversidade bacteriana do solo em resposta a diferentes plantas, fertilizantes fosfatados e calagem

    Directory of Open Access Journals (Sweden)

    Paulo da Silva

    2002-12-01

    Gram positivos não esporulados e bacilos Gram negativos que o solo com C. cajan ou não cultivado. O número de bacilos Gram positivos esporulados foi superior nas parcelas fertilizadas com fosfato do que nas não adubadas ou fertilizadas com fosfato de rocha. Nas parcelas não fertilizadas, maior número de cocos Gram positivos e bacilos Gram negativos foi obtido do que nas parcelas fertilizadas. A ausência de calagem favoreceu os bacilos Gram positivos esporulados, cocos Gram positivos e os bacilos Gram negativos, enquanto que, com calagem foram encontradas proporções maiores de bacilos Gram positivos não esporulados. De 7 a 86% do total de isolados utilizaram diferentes carboidratos. O registro dos dados utilizado neste experimento foi efetivo na identificação dos isolados e pode ser útil para o diagnóstico das bactérias do solo. Os gêneros mais freqüentes foram Bacillus, Cellulomonas, Rhodococcus, Enterobacter, Flavobacterium, Micrococcus e Arthrobacter. A diversidade bacteriana foi aumentada nas parcelas com calagem, não fertilizadas e cultivadas com plantas.

  12. Disinfectant-resistant bacteria in Buenos Aires city hospital wastewater Resistência bacteriana a desinfetantes em efluentes de um hospital em Buenos Aires, Argentina

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    L. Nuñez

    2007-12-01

    antibióticos, os desinfetantes podem atuar como agentes seletivos de linhagens resistentes aos antimicrobianos. Este trabalho teve como objetivo o estudo do perfil da resistência aos desinfetantes das bactérias lançadas na rede de esgoto pelo efluente hospitalar. Na caracterização microbiológica do efluente do Hospital de Clínicas Buenos Aires, determinou-se a concentração de bactérias heterotróficas, bactérias indicadoras fecais, Pseudomonas sp. e Staphylococcus sp. presentes. A resistência aos desinfetantes empregados no hospital, glutaraldeído, iodo povidona, e clorexidina foi então avaliada. Verificou-se a existência de bactérias resistentes à clorexidina em número variando de 10³ a 10(6 bactérias/100 mL e de bactérias resistentes a outros desinfetantes em uma faixa de variação de 10³ a 10(4 bactérias/100 mL. Bactérias dos gêneros Staphylococcus e Bacillus, e da família Enterobacteriaceae, envolvidas em infecções hospitalares, apresentaram resistência aos desinfetantes testados. Estes resultados indicam que as águas residuárias de hospitais desempenham um papel de grande relevância na disseminação de linhagens bacterianas resistentes aos desinfetantes no meio aquático.

  13. Biopolímeros, Carbono e Enxofre Totais Associados à Atividade Bacteriana dos Sedimentos Superficiais do Delta do Paraíba do Sul, RJ-Brasil

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    Lazaro Luiz Mattos Laut

    2011-01-01

    Full Text Available A bacia de drenagem do Rio Paraíba do Sul localiza-se ao longo do eixo Rio de Janeiro-São Paulo, de grande densidade demográfica e intensa industrialização. Este trabalho objetivou analisar quali-quantitativamente a matéria orgânica (biopolímeros, carbono orgânico total e enxofre e o estado metabólico bacteriano nos sedimentos superficiais estuarino-deltaicos do Rio Paraíba do Sul, relacionando-os a distribuição granulométrica. Em média, 65,5% dos sedimentos foi classificado como arenoso e empobrecidos em carbono e enxofre, cujas concentrações médias foram 0,71% e 0,04%, respectivamente. A razão elementar C:S indica que o ambiente é predominantemente oxidante, coerente com a forte influência fluvial no estuário desse rio de médio porte. Os biopolímeros carboidratos (média de 2075,93 μg C/g, proteínas (média de 25,70 μg C/g e lipídeos (média de 363,10 μg C/g também apresentaram concentrações inferiores a outros ambientes. Em função dessas baixas concentrações, pôde-se classificar o ambiente como sendo meso-oligotrófico. A razão CARBOIDRATO:COT identificou o impacto de atividades antrópicas, mais especificamente o aporte de efluentes domésticos ao longo de todo o seu curso e da indústria açucareira fortemente presente no seu baixo curso. A comunidade bacteriana, responsável pela hidrólise da matéria orgânica, é predominantemente anaeróbia e anaeróbia facultativa, implicando em perda de nitrogênio para a atmosfera (desnitrificação e produção de sulfetos (sulfato-redução e os baixos valores da razão PROTEINA:CARBOIDRATO refletem essa perda de nitrogênio (presente nos compostos protéicos considerando a ocorrência de bactérias desnitrificantes. Os valores encontrados de carbono bacteriano são elevados (3,29 μg C/g que também, provavelmente, são devidos aos impactos descritos.

  14. Documento de consenso sobre el tratamiento antimicrobiano de las infecciones bacterianas odontogénicas Consensus statement on antimicrobial treatment of odontogenic bacterial infections

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    A Bascones Martínez

    2005-12-01

    Full Text Available Las infecciones de la cavidad bucal son un problema de salud pública frecuente y motivo constante de prescripción antibiótica; el 10% de los antibióticos se emplean para tratar este problema. Sin embargo, hasta la fecha son pocos los estudios realizados para determinar su incidencia. Asímismo, su relación con ciertas enfermedades sistémicas (cardiacas, endocrinas, etc... confiere a estas patologías una importancia vital. A pesar de la reconocida frecuencia e importancia de las infecciones odontogénicas, llama la atención la actual dispersión de criterio en varios aspectos referentes a su clasificación, terminología y recomendaciones terapéuticas. El objetivo principal de este documento, realizado con el consenso de especialistas en microbiología y odontología, es establecer unas recomendaciones útiles para todos los profesionales implicados en el manejo clínico de estas patologías. Recibe especial atención el aumento de la prevalencia de resistencias bacterianas observado durante los últimos años y, en concreto, la proliferación de cepas productoras de betalactamasas. Otro factor causal importante de la aparición de resistencias es la falta de cumplimiento terapéutico, en especial en lo que respecta a la dosis y a la duración del tratamiento. Así pues, estas patologías constituyen un problema complejo cuyo abordaje requiere la instauración de antimicrobianos de amplio espectro, con adecuados parámetros farmacocinéticos, con buena tolerancia y una posología cómoda que permita que el paciente reciba la dosis adecuada durante el tiempo necesario. Amoxicilina/ácido clavulánico a dosis altas (2000mg/ 125mg ha demostrado buenos resultados y capacidad para superar resistencias. Otros agentes como metronidazol y clindamicina, seguidos de claritromicina y azitromicina han demostrado también ser activos frente a la mayoría de los microorganismos responsables de las infecciones odontogénicas.The infection of the oral

  15. Atrofia mucosa/translocação bacteriana na sepse experimental em ratos Wistar Mucosal atrophy/bacterial translocation in experimental sepsis in Wistar rats

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    Armando José d'Acampora

    2004-10-01

    Full Text Available OBJETIVO: Avalizar a relação entre lesão mucosa e translocação bacteriana. MÉTODO: Utilizou-se 50 ratos distribuídos em 5 grupos: 1.Controle: injeção de inóculo padrão de Pseudomonas aeruginosa, 2.Dreno: injeção do inóculo padrão e drenagem da cavidade abdominal, após 6 horas, 3.Lavado: injeção do inóculo padrão e lavagem da cavidade abdominal, após 6 horas, 4.Lavado + dreno: injeção do inóculo padrão e após 6 horas, drenagem e lavagem da cavidade, 5.Normal: avaliação histológica da parede intestinal normal. Após o óbito, realizou-se hemocultura e cultura peritoneal. Realizou-se medida da espessura total da parede do jejuno e da camada mucosa em vilosidades seccionadas de forma longitudinal. RESULTADOS: Na hemocultura, houve crescimento de Pseudomonas aeruginosa e Escherichia coli em 90% e 52,5% dos animais. Na cultura peritoneal, houve crescimento de P. aeruginosa, E. coli e Klebsiella sp em 87,5%, 85% e 5% dos animais. Quanto a altura da camada mucosa e da parede intestinal, não houve alteração estatisticamente significativa entre os 5 grupos. CONCLUSÃO: A sepse aguda não causou alteração na camada mucosa do intestino delgado e a translocação ocorrida não pode ser considerada como decorrente de uma lesão da mucosa intestinal.PURPOSE: Observe the relation between small intestine's mucosal injury and bacterial translocation. METHODS: 50 adult female rats were distributed in 5 groups: 1. Control: intraperitoneal injection of Pseudomonas aeruginosa, 2. Drainage: intraperitoneal injection of P. aeruginosa and drainage of the abdominal cavity, after 6 hours, 3. Washed: intraperitoneal injection of P. aeruginosa and washing of the abdominal cavity, after 6 hours, 4. Washed + drainage: intraperitoneal injection of P. aeruginosa plus drainage and washing of the cavity, after 6 hours, 5. Normal: evaluation of the normal intestinal wall. After death, blood and peritoneal cultures were performed. Fragments of

  16. Lactococcus lactis and Lactobacillus sakei as bio-protective culture to eliminate Leuconostoc mesenteroides spoilage and improve the shelf life and sensorial characteristics of commercial cooked bacon.

    Science.gov (United States)

    Comi, Giuseppe; Andyanto, Debbie; Manzano, Marisa; Iacumin, Lucilla

    2016-09-01

    Cooked bacon is a typical Italian meat product. After production, cooked bacon is stored at 4 ± 2 °C. During storage, the microorganisms that survived pasteurisation can grow and produce spoilage. For the first time, we studied the cause of the deterioration in spoiled cooked bacon compared to unspoiled samples. Moreover, the use of bio-protective cultures to improve the quality of the product and eliminate the risk of spoilage was tested. The results show that Leuconostoc mesenteroides is responsible for spoilage and produces a greening colour of the meat, slime and various compounds that result from the fermentation of sugars and the degradation of nitrogen compounds. Finally, Lactococcus lactis spp. lactis and Lactobacillus sakei were able to reduce the risk of Leuconostoc mesenteroides spoilage. PMID:27217354

  17. Classification of Lactococcus lactis cell envelope proteinase based on gene sequencing, peptides formed after hydrolysis of milk, and computer modeling

    DEFF Research Database (Denmark)

    Børsting, Mette Winther; Qvist, K.B.; Brockmann, E.; Vindeløv, J.; Pedersen, T.L.; Vogensen, Finn Kvist; Ardö, Ylva Margareta

    2015-01-01

    Lactococcus lactis strains depend on a proteolytic system for growth in milk to release essential AA from casein. The cleavage specificities of the cell envelope proteinase (CEP) can vary between strains and environments and whether the enzyme is released or bound to the cell wall. Thirty-eight Lc....... lactis strains were grouped according to their CEP AA sequences and according to identified peptides after hydrolysis of milk. Finally, AA positions in the substrate binding region were suggested by the use of a new CEP template based on Streptococcus C5a CEP. Aligning the CEP AA sequences of 38 strains...... of Lc. lactis showed that 21 strains, which were previously classified as group d, could be subdivided into 3 groups. Independently, similar subgroupings were found based on comparison of the Lc. lactis CEP AA sequences and based on normalized quantity of identified peptides released from αS1-casein...

  18. Conjuntivite bacteriana secundária à doença dentária em chinchilas (Chinchilla lanigera Bacterial conjunctivitis secondary to dental disease in chinchillas (Chinchilla lanigera

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    Ricardo Barbosa Lucena

    2012-11-01

    Full Text Available São relatados três casos de conjuntivite bacteriana associada à doença dentária grave em chinchilas. Todas as chinchilas afetadas tinham histórico de emagrecimento, hipersalivação e epífora, que progrediu para exsudação ocular purulenta. Durante a necropsia, foi constatado marcado alongamento da coroa clínica dos incisivos e molares, e crescimento do ápice dentário, causando deformação óssea e compressão do canal lacrimal. Histologicamente, observou-se infiltrado de neutrófilos na conjuntiva e pálpebras. Cultivo microbiológico do exsudato ocular revelou crescimento de Staphylococcus coagulase-positiva . Uma compressão do canal lacrimal pelo crescimento dentário excessivo impediu a drenagem das lágrimas, resultando em epífora. Esse é um importante fator predisponente para infecção bacteriana ocular em chinchilas.Three cases of bacterial conjunctivitis associated with severe dental disease in chinchillas are described. All affected chinchillas had a history of weight loss, ptyalism, and epiphora which progressed to suppurative ocular exsudation. At necropsy incisor and molar teeth revealed marked elongation of the clinical crown and overgrowth of the dental apexes resulting in deformation of the tear ducts. Histologically, there was neutrophilic infiltrate in the conjunctiva and eyelid skin. Microbiological culture carried out in samples from the ocular exsudate yielded Staphylococcus coagulase-positive. Compression of the lacrimal duct by dental overgrowth compromised tear draining and resulting in epiphora. This is a major predisposing factor inducing bacterial ocular infection in chinchillas.

  19. QUALIDADE DO LEITE CRU REFRIGERADO ESTOCADO POR DIFERENTES PERÍODOS

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    Priscila Alonso dos Santos

    2008-10-01

    Full Text Available O objetivo da presente pesquisa foi avaliar a qualidade do leite cru refrigerado estocado por diferentes períodos. As amostras de leite cru refrigerado foram obtidas diretamente de 10 tanques de expansão individuais localizados em propriedades leiteiras da região Sudoeste do Estado de Goiás, seguindo-se uma rota determinada pela indústria devidamente registrada no Serviço de Inspeção Federal. Após definida a rota, as coletas foram realizadas acompanhando o período de armazenamento do leite por zero, 24, 48 e 72 horas, perfazendo um total de 40 amostras. As análises foram realizadas no Laboratório de Qualidade do Leite do Centro de Pesquisa em Alimentos da Escola de Veterinária da Universidade Federal de Goiás, no período de janeiro a fevereiro de 2008. A contagem de células somáticas (CCS foi realizada através de citometria de fluxo pelo equipamento Fossomatic 5000 BasicÒ. A composição centesimal foi determinada através da absorção diferencial de ondas infravermelhas utilizando-se o equipamento Milkoscan 4000Ò. A contagem bacteriana total (CBT foi realizada por meio do equipamento Bactoscan FCÒ, cuja análise baseia-se na citometria de fluxo. A análise de resíduos de antibióticos foi realizada em todas as amostras através do DELVOTEST® SP. As análises estatísticas foram realizadas de acordo com o pacote estatístico R (2005 utilizandose os procedimentos para estatística descritiva. Os valores encontrados para a CCS e composição centesimal atendem aos padrões exigidos pela legislação brasileira. A CBT apresentou valores elevados sendo necessária a implantação de boas práticas na obtenção e conservação do leite cru refrigerado. Não foi detectada a presença de resíduos de antibióticos nas amostras analisadas.

  20. The ltp gene of temperate Streptococcus thermophilus phage TP-J34 confers superinfection exclusion to Streptococcus thermophilus and Lactococcus lactis

    International Nuclear Information System (INIS)

    The ltp gene, located within the lysogeny module of temperate Streptococcus thermophilus phage TP-J34, has been shown to be expressed in lysogenic strain S. thermophilus J34. It codes for a lipoprotein, as demonstrated by inhibition of cleavage of the signal sequence by globomycin. Exposure of Ltp on the surface of Lactococcus lactis protoplasts bearing a plasmid-encoded copy of ltp has been demonstrated by immunogold labeling and electron microscopy. Expression of ltp in prophage- and plasmid-cured S. thermophilus J34-6f interfered with TP-J34 infection. While plating efficiency was reduced by a factor of about 40 and lysis of strain J34-6f in liquid medium was delayed considerably, phage adsorption was not affected at all. Intracellular accumulation of phage DNA was shown to be inhibited by Ltp. This indicates interference of Ltp with infection at the stage of triggering DNA release and injection into the cell, indicating a role of Ltp in superinfection exclusion. Expression of ltp in L. lactis Bu2-60 showed that the same superinfection exclusion mechanism was strongly effective against phage P008, a member of the lactococcal 936 phage species: no plaque-formation was detectable with even 109 phage per ml applied, and lysis in liquid medium did not occur. In Lactococcus also, Ltp apparently inhibited phage DNA release and/or injection. Ltp appears to be a member of a family of small, secreted proteins with a 42 amino acids repeat structure encoded by genes of Gram-positive bacteria. Some of these homologous genes are part of the genomes of prophages