WorldWideScience

Sample records for bacteriana del semen

  1. Flora bacteriana del semen de toro antes y después de la congelación (Bacterial flora of bull semen before and after freezing process

    Directory of Open Access Journals (Sweden)

    Enrique A. Silveira Prado

    2005-10-01

    Full Text Available Se investigó por bacteriología general el semen fresco y después de la congelación de 50 toros de inseminación artificial y se efectuó el conteo total de unidades formadoras de colonias (UFC. A l5 de los toros se les realizó el examen bacteriológico de sus lavados prepuciales. En todas las muestras de semen fresco se obtuvo crecimiento bacteriano y los gérmenes más frecuentemente aislados fueron: Escherichia coli (50,0%, Staphylococcus aureus (36,0% y Staphylococcus coagulasa negativa (28,0%. En el semen congelado solamente se obtuvo crecimiento en el 20,0%. El 74,0% del semen fresco alcanzó conteos  1 x 104 UFC/mL antes de ser procesado; después de la congelación el 80,0% fue estéril. En el total de lavados prepuciales se obtuvo crecimiento y se detectó en mayor proporción el Staphylococcus coagulasa negativa (60,0%, microorganismo también aislado en el semen fresco de estos toros. Se concluyó que la adición de antibióticos al menstruo y posterior congelación en pastillas, disminuye notablemente la carga microbiana presente en el semen. It was investigated through general bacteriology both fresh semen and after the freezing process, carried out in 50 bulls of artificial insemination, total counting of colony forming units (CFU was made. A bacteriological analysis of the prepucial washing was made on 15 of these bulls. In all samples of fresh semen there was bacterial growing. The most frequently germs were: Escherichia coli (50,0%, Staphylococcus aureus (36,0% and coagulase negative Staphylococcus (28,0%. In samples of frozen semen growth was only obtained in the 20,0%. The 74,0% of samples of fresh semen reached counts  1 x 104 CFU/mL before being processed; after freezing 80,0% of the samples were sterile. In all prepucial washings it was obtained growth and mostly detected coagulase negative Staphylococcus (60.0%, was also isolated in the fresh semen of these bulls. We concluded that the addition of antibiotics to

  2. Flora bacteriana del semen de toro antes y después de la congelación (Bacterial flora of bull semen before and after freezing process)

    OpenAIRE

    Silveira Prado, Enrique A; Roberto Machado Pérez

    2005-01-01

    Se investigó por bacteriología general el semen fresco y después de la congelación de 50 toros de inseminación artificial y se efectuó el conteo total de unidades formadoras de colonias (UFC). A l5 de los toros se les realizó el examen bacteriológico de sus lavados prepuciales. En todas las muestras de semen fresco se obtuvo crecimiento bacteriano y los gérmenes más frecuentemente aislados fueron: Escherichia coli (50,0%), Staphylococcus aureus (36,0%) y Staphylococcus coagulasa negativa (28,...

  3. Perdurabilidad de la efectividad de la combinación penicilina-estreptomicina en la reducción de la carga bacteriana del semen de toro congelado en pastillas (Durability of the effectiveness of the penicillin-streptomycin combination in the reduction of the bacterial content of frozen bulls semen in concentrated pellet form)

    OpenAIRE

    2005-01-01

    Se investigaron por bacteriología general 206 muestras de semen fresco, 133 de semen diluido y congelado en forma de pastillas con adición de penicilina-estreptomicina y 33 sin antibióticos, de diferentes toros de inseminación artificial clínicamente sanos. El resumen de los aislamientos agrupados según las especies bacterianas reveló que las especies más frecuentemente aisladas en muestras de semen fresco fueron E. coli (50,6%), otras enterobacterias (27,7%), Staphylococcus coag. neg. (26,2%...

  4. Perdurabilidad de la efectividad de la combinación penicilina-estreptomicina en la reducción de la carga bacteriana del semen de toro congelado en pastillas (Durability of the effectiveness of the penicillin-streptomycin combination in the reduction of the bacterial content of frozen bulls semen in concentrated pellet form

    Directory of Open Access Journals (Sweden)

    Luis O. Alba Gómez

    2005-07-01

    Full Text Available Se investigaron por bacteriología general 206 muestras de semen fresco, 133 de semen diluido y congelado en forma de pastillas con adición de penicilina-estreptomicina y 33 sin antibióticos, de diferentes toros de inseminación artificial clínicamente sanos. El resumen de los aislamientos agrupados según las especies bacterianas reveló que las especies más frecuentemente aisladas en muestras de semen fresco fueron E. coli (50,6%, otras enterobacterias (27,7%, Staphylococcus coag. neg. (26,2% y S. aureus (17,5%. En el semen congelado sin antibióticos y congelado con antibióticos estas bacterias fueron igualmente las predominantes. Se observó disminución de la frecuencia de aislamientos en las muestras de semen congelado sin antibióticos (78,8% que se hizo más notable en las muestras con antibióticos (18,8%, diferencias estadísticamente significativas

  5. Fertilidad y supervivencia del semen canino criopreservado

    OpenAIRE

    Stornelli, María Alejandra; Sota, Rodolfo Luzbel de la

    2006-01-01

    En la presente revisión se tratan los procedimientos de evaluación seminal, los aspectos más importantes de la criopreservación de semen en el perro y el alcance de la valoración in vitro de la calidad de semen al descongelado. Se realiza también la evaluación de los alcances de la inseminación artificial con semen criopreservado en esta especie.

  6. IDENTIFICACIÓN MOLECULAR DE POBLACIONES BACTERIANAS ASOCIADAS AL CARACOL PALA (Strombus gigas DEL CARIBE COLOMBIANO

    Directory of Open Access Journals (Sweden)

    EDINSON ANDRÉS ACOSTA

    2009-01-01

    Full Text Available El caracol Pala, Strombus gigas (Strombidae, es de gran importancia ecológica y so- cioeconómica en el área caribeña colombiana. Sin embargo, es una especie catalogada como "vulnerable" y existe muy poca información referente a las especies bacterianas asociadas al caracol que puedan ser importantes para el desarrollo, manejo productivo y de seguridad acuícola de estos gastrópodos. En este trabajo, nosotros empleamos un estudio microbiológico y molecular de la región intergénica entre los genes 16S y 23S rDNA, análisis del gen rDNA 16S y secuenciación, para analizar las bacterias asociadas al caracol Pala (S. gigas. La composición de bacterias cultivables asociadas fue evaluada por su capacidad para crecer en agar marino y en medios de cultivos selectivos. De un total de 28 muestras analizadas encontramos que el número de bacterias cultivadas en condiciones aerobias fue de alrededor 106 ufc mL-1 donde las bacterias pertenecientes a la familia Vibrionacea fueron las más abundantes, cerca de >105 ufc mL-1 . El análisis molecular de la región intergénica entre los genes 16S y 23S rDNA de las diferentes muestras, reveló una gran complejidad bacteriana asociada a S. gigas. Las secuencias de los amplificados del gen rDNA 16S identificó Pseudoalteromonas sp., Halomonas sp., Psycrobacter sp., Cobetia sp., Pseudomonas sp. y Vibrios sp. Nuestros resultados podrían sugerir un rol importante de estas bacterias como componentes de la comunidad asociada al S. gigas. Esta información puede complementar los estudios que se están implementando en los procesos para la conservación y repoblamiento de las poblaciones de S. gigas en Colombia.

  7. Guías de manejo de las queratitis infecciosas bacterianas. Hospital Universitario del Valle, enero-julio 2002 .

    Directory of Open Access Journals (Sweden)

    Alejandro de La Torre

    2009-11-01

    Full Text Available Propósito. Evaluar los resultados de la aplicación de las guías de manejo de las queratitis infecciosas bacterianas en pacientes que llegan al Servicio de Oftalmología. Métodos. Ingresaron con diagnóstico de queratitis infecciosas bacterianas 21 pacientes durante 6 meses. Resultados. De 18 queratitis infecciosas, 16 (88.9% se manejaron medicamente y 2 se les realizó tratamiento quirúrgico; 50% (9 se diagnosticaron como severas, 14 cumplieron criterios para tomar muestras de laboratorio: sensibilidad del Gram es de 50% y la sensibilidad de cultivos para bacterias es de 64.2%. La bacteria encontrada con más frecuencia fue el Staphylococcus epidermidis. Conclusión. El estudio demostró evidentemente que las guías de manejo propuestas para las queratitis infecciosas bacterianas son útiles para lograr su recuperación sin complicaciones (prueba exacta de Fisher p= 0.001032.

  8. IDENTIFICACIÓN MOLECULAR DE POBLACIONES BACTERIANAS ASOCIADAS AL CARACOL PALA (Strombus gigas DEL CARIBE COLOMBIANO

    Directory of Open Access Journals (Sweden)

    ROMERO MAGALLY

    2009-08-01

    Full Text Available RESUMEN

    El caracol Pala, Strombus gigas (Strombidae, es de gran importancia ecológica y socioeconómica en el área caribeña colombiana. Sin embargo, es una especie catalogada como “vulnerable” y existe muy poca información referente a las especies bacterianas asociadas al caracol que puedan ser importantes para el desarrollo, manejo productivo y de seguridad acuícola de estos gastrópodos. En este trabajo, nosotros empleamos un estudio microbiológico y molecular de la región intergénica entre los genes 16S y 23S rDNA, análisis del gen rDNA 16S y secuenciación, para analizar las bacterias asociadas al caracol Pala (S. gigas. La composición de bacterias cultivables asociadas fue evaluada por su capacidad para crecer en agar marino y en medios de cultivos selectivos. De un total de 28 muestras analizadas encontramos que el número de bacterias cultivadas en condiciones aerobias fue de alrededor 106 ufc mL-1 donde las bacterias pertenecientes a la familia Vibrionacea fueron las más abundantes, cerca de >105 ufc mL-1 . El análisis molecular de la región intergénica entre los genes 16S y 23S rDNA de las diferentes muestras, reveló una gran complejidad bacteriana asociada a S. gigas. Las secuencias de los amplificados del gen rDNA 16S identificó Pseudoalteromonas sp., Halomonas sp., Psycrobacter sp., Cobetia sp., Pseudomonas sp. y Vibrios sp. Nuestros resultados podrían sugerir un rol importante de estas bacterias como componentes de la comunidad asociada al S. gigas. Esta información puede complementar los estudios que se están implementando en los procesos para la conservación y repoblamiento de las poblaciones de S. gigas en Colombia.

    Palabras clave: Strombus gigas, Caracol pala, Bacteria, Región intergénica 16S-23S, rDNA 16S.

    ABSTRACT

    The Queen Conch, Strombus gigas (Strombidae, is a species of great ecological and socioeconomic importance in the Caribbean area of Colombia

  9. Efecto del plasma seminal sobre el estado redox del semen equino criopreservado

    Directory of Open Access Journals (Sweden)

    Edison Pizarro L.

    2013-10-01

    Full Text Available Objetivo. Determinar el efecto del plasma seminal sobre la generación de especies reactivas de oxígeno (ERO y la peroxidación lipídica de semen equino criopreservado y su asociación con parámetros de calidad seminal. Materiales y métodos. El semen de cinco caballos de la raza criollo colombiano (dos eyaculados cada uno, fue criopreservado mediante un protocolo de congelación rápida, empleando un diluyente leche-yema de huevo, suplementado con 0%, 10% y 20% de plasma seminal equino. En muestras de semen fresco y criopreservado se evaluó la generación de ERO y la peroxidación lipídica por espectrofluorimetría, y los parámetros de calidad seminal de movilidad progresiva, vitalidad e integridad de membrana, mediante microscopia de contraste de fase. Para el análisis estadístico se ajustaron modelos mixtos y se realizaron análisis de regresión y correlación. Resultados. Se hallaron promedios post-descongelación de movilidad progresiva, vitalidad e integridad de membrana de 37.8%±20.2, 50.6% ± 14.6 y 37.8% ± 15.5, respectivamente. Para el semen fresco y criopreservado suplementado con 0%, 10% y 20% de plasma seminal, los promedios de producción de ERO (URF fueron de 13.34±10.7, 16.15 ± 13.5, 17.32 ± 16 y 22.98 ± 19.4, respectivamente; mostrando un incremento estadísticamente significativo (p≤0.05 en la producción de ERO por efecto de la criopreservación y la suplementación con plasma seminal. Los promedios de peroxidación lipídica (nmolMDA/ml para estos mismos tratamientos, fueron de 0.41 ± 0.25, 0.72±0.37, 0.51 ± 0.29 y 0.47±0.26, respectivamente; mostrando una reducción significativa (p≤0.05 de la peroxidación lipídica del semen suplementado con 10% y 20% de plasma seminal, respecto al semen no suplementado (0%. Conclusiones. El plasma seminal reduce la peroxidación lipídica del semen equino criopreservado.

  10. Encefalitis bacteriana e hidrocefalia

    OpenAIRE

    Iglesias Rozas, José Rafael, 1942-

    1982-01-01

    Nueve imágenes de una encefalitis bacteriana en un niño con hidrocefalia por oclusión del acueducto de Silvio. Nine pictures of a bacterial encephalitis in a child with hydrocephalus caused by an occlusion of the Sylvian aqueduct.

  11. Estudio comparativo de dos tratamientos con antibióticos sobre la calidad bacteriológica y espermática del semen bovino.

    Directory of Open Access Journals (Sweden)

    Trujillo A Luis Emilio

    2002-06-01

    Full Text Available Para investigar el efecto antibacteriano y sobre la calidad del semen bovino se aplicaron dos tratamientos con antibióticos al diluyente de conservación. Una terapia consideró el uso de una nueva combinación de antibióticos (100 μg/ml de tilosina, 500 μg/ml de gentamicina, 300 μg/ml de lincomicina y 600 μgl/ml de espectinomicina y la otra utilizó la combinación tradicional de 1000 Ul/ml de penicilina y 1 μg/ml de sulfato de estreptomicina. La investigacion se realizó en el Laboratorio de Procesamiento de Semen de San Pablo de la Universidad Nacional de Colombia Sede Medellín bajo condiciones bioclimáticas correspondientes a una zona de vida de bosque húmedo montano bajo (bh-MB. El semen recibió el tratamiento antibiótico en la fracción A del diluyente a una temperatura de 32C y se le permitió interactuar por espacio de 10 minutos antes de darse inicio al descenso de la temperatura hasta 5C, momento en el cual se adicionó la fracción B del diluyente con el crioprotector, se estabilizó durante tres horas y se congeló en pajillas francesas de 0,5 ml. Para efecto de la evaluación del crecimiento bacterial e identificación de las colonias las muestras fueron sometidas a cultivo y aislamiento en agar sangre y agar MacConkey. La calidad seminal se evaluó por el porcentaje de espermas móviles, porcentaje de espermas vivos y morfología espermática. El efecto de los tratamientos se analizó por análisis de varianza y los promedios fueron comparados por la prueba de los rangos múltiples de Duncan. El 62,5% de las muestras presentaron contaminación bacteriana por Escherichia coli, Proteus spp o Klebsiella spp.y el promedio de unidades formadoras de colonias (UFC en las muestras contaminadas fue de 6,416 UFC/ml de semen, variando entre cero (0 y 30.000. Los dos tratamientos con antibióticos ejercieron un efecto bactericida que permaneció durante el proceso de congelación y descongelación del semen. No se presentaron

  12. Prevalencia de vaginosis bacteriana y factores asociados en veinte ciudades del Perú

    Directory of Open Access Journals (Sweden)

    Lorena López-Torres

    Full Text Available Objetivos. Determinar la prevalencia de vaginosis bacteriana (VB y factores asociados en mujeres peruanas de 18 a 29 años de edad en 20 ciudades a partir de datos del proyecto PREVEN. Materiales y métodos. Estudio de tipo transversal, la definición de VB se realizó previa selección de una muestra de secreción vaginal en una lámina portaobjetos. Las láminas fueron teñidas usando la tinción Gram para ser observadas al microscopio usando el puntaje de Nugent, el diagnóstico de VB se aplicó a los puntajes 7-10. Se estimaron razones de prevalencias (RP y sus intervalos de confianza al 95% (IC 95% mediante el uso de modelos lineales generalizados. Resultados. Un total de 6322 mujeres contestaron la encuesta epidemiológica y proporcionaron muestras vaginales. La prevalencia de VB fue de 23,7% (IC95%: 22,6-24,7 y se asoció con tener un mayor número de parejas sexuales en los últimos 12 meses (RP: 1,22, IC 95%: 1,03-1,44, p=0,020; para dos parejas y RP: 1,46, IC 95%: 1,23-1,74, p<0,001 para tres o más parejas, no usar condón en la última relación sexual (RP: 1,16, IC 95%: 1,01-1,34, p=0,034, ser residente de la sierra (RP: 1,18, IC 95%: 1,05-1,31, p=0,004 y tener flujo vaginal anormal o con mal olor (RP: 1,20, IC 95%: 1,09-1,33, p<0,001. Conclusiones. La alta prevalencia de VB encontrada remarca la necesidad de fortalecer los servicios de salud para la detección y tratamiento de esta condición.

  13. Validación del método LAL para determinar endotoxinas bacterianas en el inyectable heparina sódica

    Directory of Open Access Journals (Sweden)

    Nancy Burguet

    2012-12-01

    Full Text Available Dentro del control de la calidad de los productos farmacéuticos, la United State Pharmacopeia establece la cuantificación de endotoxinas bacterianas por el método de lisado de amebocito de Limulus , como monitor de pirógenos para más del 90% de los parenterales que regula. Este método se aplicó de forma específica a vacunas bacterianas y virales, agentes antineoplásicos, radiofármacos y parenterales que se producen en la industria médico farmacéutica. En el presente trabajo se mostró la metodología a seguir para realizar la validación de la técnica de determinación de endotoxinas bacterianas por el método de gelificación. Para ello se confirmó la sensibilidad del reactivo utilizado (0,03125 UE/mL y la validez de los analistas para poder obtener resultados confiables. Las pruebas preliminares para el producto ensayado, heparina sódica 5000 UI/mL, demostraron que este producto no potencia ni inhibe la reacción del reactivo. Se escogió la dilución de trabajo (1/128 para la validación del método. De esta manera quedaron estandarizadas las condiciones para la validación del test de lisado de amebocito de Limulus por gelificación en este producto parenteral, método que se hace extensivo a la determinación de endotoxinas bacterianas en vacunas y en otros medicamentos por vía de administración intravenosa.

  14. Desarrollo de la comunidad bacteriana del floc biológico de barros activados y respuesta a la desestabilización mediada por agentes externos

    OpenAIRE

    2013-01-01

    Ayarza, J. M. (2013). Desarrollo de la comunidad bacteriana del floc biológico de barros activados y respuesta a la desestabilización mediada por agentes externos (Tesis de Doctorado). Universidad Nacional de Quilmes, Bernal, Argentina. El tratamiento de efluentes basado en barros activados es el proceso más ampliamente empleado en todo el mundo para el tratamiento biológico de efluentes de origen industrial o domiciliario. El éxito del proceso radica no sólo en la selección de poblacion...

  15. La mancha bacteriana del champiñón y otras setas cultivadas

    OpenAIRE

    2011-01-01

    El cultivo de champiñón y otras setas cultivadas se ve afectado por enfermedades de origen bacteriano que pueden causar pérdidas de producción importantes. Para controlar esta enfermedad es fundamental realizar un manejo adecuado del cultivo evitando el exceso de humedad.

  16. Obtención y evaluación del semen de capibara Hydrochoerus hydrochaeris

    Directory of Open Access Journals (Sweden)

    José Rodríguez P.

    2012-08-01

    Full Text Available Objetivo. Determinar las características del semen y la morfometría de los espermatozoides del Capibara (Hydrochoerus hydrochaeris. Materiales y métodos. Se utilizaron 10 machos con peso entre 21-45 kg, los cuales fueron restringidos y anestesiados. El semen se obtuvo mediante electroeyaculación y se determinó el color, volumen, pH, motilidad en masa, motilidad individual, viabilidad, concentración y morfología. Se realizaron además mediciones de la cabeza y la cola de los espermatozoides. Resultados. Se obtuvo semen en el 100% (10/10 de los animales. El mayor número de eyaculaciones (80%; 8/10, se obtuvo con un voltaje máximo de 6V. El color fue blanco, de aspecto lechoso, los valores promedio fueron volumen 135.5±93.56 μl, pH 8.14±0.38, motilidad masal 32.60±13.46%, motilidad individual 34±19.81%, viabilidad 51.3±19.42%, concentración espermática 127±59.01x106 espermatozoides/mL y morfología 51.3±19.42 espermatozoides normales. La longitud de la cabeza fue 5.41±0.7 μm, el ancho de la cabeza 3.77±0.5 μm y área de la cabeza 75.66±20.6 μm2. La longitud de la cola fue 27.9±11.3 μm. Conclusiones. La obtención del semen fue satisfactoria mediante electroeyaculación, sin presentar notables diferencias en las características del semen y morfología de los espermatozoides con otros roedores silvestres de menor tamaño, aunque se observó una alta variabilidad de estas características entre los animales muestreados posiblemente por la heterogeneidad de los animales experimentales.

  17. AMONIO-OXIDASAS BACTERIANAS Y ARQUEALES INVOLUCRADAS EN EL CICLO DEL NITRÓGENO

    Directory of Open Access Journals (Sweden)

    Sergio Andrade Ochoa

    2015-09-01

    Full Text Available La nitrificación, la oxidación microbiana de amoníaco a nitrato, juega un papel crítico en el ciclo global del nitrógeno. Se ha considerado a dos grupos distintos de bacterias capaces de oxidar el amoníaco (AOB como las responsables de llevar a cabo el primer paso de la nitrificación; en esos dos grupos se encuentran las Beta-proteobacterias, que incluyen a los géneros Nitrosomonas y Nitrosospira, y las Gamma-proteobacterias, que engloban al género Nitrosococcus. Sin embargo, se ha demostrado la existencia de arqueas oxidantes de amoníaco (AOA y su contribución al ciclo del nitrógeno, siendo Nitrosopumilus maritimus la más estudiada en este proceso. La dinámica entre estos dos sistemas de oxidantes de amoníaco es compleja y los estudios se han enfocado a distintos hábitats, se ha propuesto que las contribuciones relativas de las comunidades AOA y AOB dependen de propiedades fisicoquímicas del ambiente, que afectan su abundancia y diversidad; esto dificulta establecer con exactitud el nicho ecológico en el que se desempeña cada comunidad. El objetivo de esta revisión es discutir la evidencia científica que facilite el entendimiento del papel que desempeñan los microorganismos oxidantes del amoníaco.

  18. Inoculación bacteriana en el crecimiento y calidad del fruto de cinco variedades de fresa en suelos con pH contrastante

    Directory of Open Access Journals (Sweden)

    José Alberto Ortiz Texon

    2016-04-01

    Full Text Available En un primer experimento se determinó el crecimiento de cinco variedades de fresa en dos suelos con pH contrastante (4.4 y 8.8. La producción de materia seca de la parte aérea, área foliar, volumen radical y concentración de N, P, K en la parte aérea se determinaron 125 días después del trasplante (ddt. Las plantas de todas las variedades cultivadas en pH 4.4 superaron a las cultivadas en pH 8.8 en todas las variables. Las variedades CP-06-15 y Festival fueron las que mejor se adaptaron a las condiciones de pH alcalino. En un segundo experimento se determinó la función de la inoculación con rizobacterias solubilizadoras de fosfatos en la reducción de los efectos negativos del pH alcalino en las variedades de fresa. Dos tipos de inoculación al momento del trasplante fueron comparadas: aplicación con pipeta de precisión e inmersión de las raíces en la suspensión bacteriana; además del testigo (sin inoculación. Se determinó crecimiento de las plantas y la producción y calidad de los frutos. La inoculación bacteriana no tuvo efecto en el crecimiento de la parte aérea y la raíz de las plantas, excepto en la variedad más sensible al pH alcalino (CP-LE-07 inoculada por inmersión de la raíz. También, esta fue la única variedad en la que la inoculación bacteriana aumentó el número de frutos producidos. De acuerdo al promedio de todas las variedades, la inoculación bacteriana, en ambas de sus formas, redujo el número y peso de los frutos producidos.

  19. EFICACIA DEL CEPILLADO CON Y SIN DENTÍFRICO EN EL CONTROL DE LA PLACA BACTERIANA SUPRAGINGIVAL EN ALUMNOS DEL 1ER GRADO DEL NIVEL PRIMARIO DE LA INSTITUCIÓN EDUCATIVA JUAN MANUEL POLAR DEL DISTRITO DE JOSÉ LUIS BUSTAMANTE Y RIVERO. AREQUIPA, 2015.

    OpenAIRE

    CÁRDENAS DEL CARPIO, ROSA ELENA

    2016-01-01

    CEPILLADO OBJETIVOS FRECUENCIA DE CEPILLADO DURACIÓN DEL CEPILLADO TÉCNICAS DE CEPILLADO REQUISITOS DE UN CEPILLADO DE DIENTES SATISFACTORIO DENTÍFRICO O PASTA DENTAL PLACA BACTERIANA ÍNDICE DE HIGIENE ORAL SIMPLIFICADO ANÁLISIS DE ANTECEDENTES INVESTIGATIVOS

  20. Características seminales del macho cabrío Serrano Transmontano (Semen characteristics of the Serrano Transmontano buck

    Directory of Open Access Journals (Sweden)

    Almendra, L.

    2005-12-01

    Full Text Available Las características de producción espermática constituyen uno de los factores más importantes del desarrollo de programas de inseminación artificial. El objetivo del presente trabajo consistió en el estudio de algunas de las características del semen producido a lo largo del año por machos cabríos de la raza Serrana Ecotipo Transmontano. Fueron estudiados 8 animales (4 adultos datando la primera recogida de semen con más de 18 meses y 4 machos jóvenes con 6-10 meses de edad. Las variables analizadas fueron el volumen (n=378; 0,859 ± 0,337 ml, la concentración (n=227; 4,791 x 106 ± 1,694 espermatozóides/ml, la motilidad masal (n=314; 3,7 ± 0,8 y la motilidad individual (n=308; 69,2 ± 14,1 %. Se observó una influencia muy significativa (P0,05. A pesar de las variaciones observadas, las características seminales (volumen de eyaculado y motilidad espermática, no parecen constituir un factor adverso en la utilización del semen en inseminación artificial, cualquiera que sea la época del año. Sperm production is one of the most important factors for the development of artificial insemination programs. The objective of this study was the evaluation of some characteristics of semen produced throughout the year by bucks of the breed Serrana ecotype Transmontano. Eight male goats were studied (4 adult males, more than 18 months old at the first semen collection and 4 young males 6-10 months old. Variables studied were volume of ejaculate (n=378; 0.859 ± 0.337 ml, sperm number (n=227; 4.791 x 106 ± 1.694 sperm cells/ml, sperm mass motility (n=314; 3.7 ± 0.8 and sperm individual motility (n=308; 69.2 ± 14.1 %. Age of males influenced significantly (P0,05. In spite of the observed variations, the seminal characteristics (volume of the ejaculate and sperm motility don't seem to constitute an adverse factor to the use of the semen in artificial insemination in any season throughout the year

  1. Relación entre el nivel de motricidad fina y remoción de placa bacteriana a través del cepillado manual en niños

    OpenAIRE

    Macote Orosco, Lisbeth Melchora

    2015-01-01

    Introducción: En la eficacia del cepillado intervienen múltiples factores como el tiempo, zonas a cepillar y habilidades motoras de la persona. ¿El niñ@ de cinco años de edad tiene las habilidades motoras pra realizar un cepillado dental eficaz?.Objetivos: Determinar la relación entre el nivel de motricidad fina y la remoción de placa bacteriana a través del cepillado manual en niñ@s de 5 años de edad. determinar la higiene oral antes y después del cepillado, relacionar el nivel de motricidad...

  2. Relación entre el nivel de motricidad fina y remoción de placa bacteriana a través del cepillado manual en niños

    OpenAIRE

    Macote Orosco, Lisbeth Melchora

    2016-01-01

    Introducción: En la eficacia del cepillado intervienen múltiples factores como el tiempo, zonas a cepillar y habilidades motoras de la persona. ¿El niñ@ de cinco años de edad tiene las habilidades motoras pra realizar un cepillado dental eficaz?.Objetivos: Determinar la relación entre el nivel de motricidad fina y la remoción de placa bacteriana a través del cepillado manual en niñ@s de 5 años de edad. determinar la higiene oral antes y después del cepillado, relacionar el nivel de motricidad...

  3. Antibióticos vs. resistencia bacteriana

    OpenAIRE

    Elso Manuel Cruz Cruz

    2015-01-01

    La resistencia bacteriana a los antibióticos es un problema de salud mundial que se encuentra en constante evolución. De manera frecuente se reportan nuevos mecanismos de resistencia bacteriana a los antibióticos, tanto en bacterias gram negativas como en gram positivas. La presencia de resistencia en una bacteria causante de infección disminuye las posibilidades de obtener la curación clínica y la erradicación bacteriológica e incrementa los costos del tratamiento, la morbilidad y la mortali...

  4. MOMENTO DE ADICIÓN DEL GLICEROL SOBRE LA CALIDAD ESPERMÁTICA EN LA CRIOPRESERVACIÓN DE SEMEN CANINO.

    OpenAIRE

    Carlotto R., Gino; Laboratorio de Reproducción Animal, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima-Perú.; Fernández A., Víctor; Clínica de Animales Menores, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima-Perú.; Lira M., Boris; Laboratorio de Fisiología Animal, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima-Perú.

    2011-01-01

    El objetivo del presente trabajo fue evaluar el efecto del momento de adición del glicerol durante el proceso de enfriamiento sobre la calidad espermática del semen canino. Se utilizaron 15 eyaculados de 4 perros adultos. Cada muestra de semen fue dividida en alícuotas para someterlas a tres métodos de adición de glicerol (T1: al inicio; T2: al inicio y final; T3: al final de la curva de enfriamiento). El semen se envasó en pajillas de 0.5 ml y se congeló en nitrógeno líquido. Se evaluó la mo...

  5. Consideraciones prácticas acerca de la calidad del semen de conejos aplicado en estudios de toxicología de la fertilidad (Practice considerations about the semen quality of rabbits for applied in fertility toxicology study.

    Directory of Open Access Journals (Sweden)

    Arencibia Arrebola Daniel Francisco

    2009-08-01

    Full Text Available ResumenEl conejo doméstico es un descendiente del conejo salvaje que habitaba en el oeste de Europa y en el noroeste de África. En los estudios de toxicología experimental se destaca su uso en la determinación de toxicidad por irritación dérmica, ocular, toxicología de vacunas, además de ser básico como modelo animal para determinar compuestos teratogénicos, los cuales influyen tanto en la fertilidad de la hembra como en la del macho. Los métodos para la valoración de la calidad del semen, tanto para la inseminación artificial como en la investigación, están sufriendo un constante desarrollo para intentar estimar con mayor precisión la fertilidadde los machos. Desafortunadamente, las valoraciones de laboratorio nopredicen con exactitud la fertilidad y tampoco se obtiene una repetitividad de unos análisis a otros, debido a la subjetividad de muchas de dichas valoraciones. El objetivo de este trabajo es la confección de una guía teórico-práctica que permita realizar estudios del semen en conejos de la raza Nueva Zelanda Blanca, aplicado a la temática de toxicología de la fertilidad. Trataremos los temas de inducción del celo en las hembras, condiciones y método para la extracción del semen, pruebas macroscópicas y microscópicas del semen, valoración del peso de órganos y examen anatomopatológico. Todas las pruebas descritas aportan datos que nosindicarán una disminución de la calidad del semen, pero debemos realizar valoraciones que nos aporten sencillez, rapidez y que sean económicamente viables; para ello cada investigador debe utilizar aquellas que mejor se adapten a sus condiciones de trabajo.SummaryThe domestic rabbit is descendend from the wilds rabbits was to inhabited of western Europe and Northwestern Africa. The rabbits is very usefull in the experimental Toxicology study, in dermal irritation, ocular irritation and the vaccines Toxicology, beside is a basic animal models in teratogenicity evaluation of

  6. Resistencia bacteriana a los antibióticos

    OpenAIRE

    Crispín Pérez, Victor

    2014-01-01

    El descubrimiento de la antibiosis "in vitro" y el desarrollo de los antibióticos generó la ilusión del control de las enfermedades infecciosas bacterianas. Sin embargo, tan pronto como estas moléculas maravillosas fuero introducidas en la clínica, casi de inmediato surgieron las cepas bacterianas con resistencia adquirida, como se ha comprobado en las colecciones de cepas aisladas en las décadas del 50 del siglo pasado. Las moléculas de antibióticos inhiben o matan a las cepas sensibles, inh...

  7. Meningitis bacteriana Bacterial meningitis

    Directory of Open Access Journals (Sweden)

    Ana Teresa Alvarado Guevara

    2006-03-01

    Full Text Available En Costa Rica la meningitis bacteriana se ha convertido en un tema prioritario en lo que a vigilancia epidemiológica se refiere, en los últimos meses se ha dado un aumento en la atención pública de este tema, debido a este fenómeno se hace necesario realizar una revisión del tema. La meningitis es una inflamación de las leptomeninges y colonización del líquido cefalorraquídeo (LCR debido a diferentes agentes, lo cual produce síntomas meníngeos (Ej., cefalea, rigidez nucal, fotofobia y pleocitosis a nivel de LCR. Dependiendo de las variables se pueden agrupar en diferentes clasificaciones, tomando en cuenta el tiempo de evolución se pueden dividir en agudas o crónicas, a las primeras con pocas horas o días de inicio de la sintomatología, mientras que la crónica presenta un curso mas larvado de la enfermedad de aproximadamente 4 semanas de instauración. Existe también diferencia según su etiología, pueden ser infecciosas y no infecciosas. Causas no infecciosas incluyen: drogas antiinflamatorias, antibióticos y carcinomatosis. A su vez existe una clasificación según el agente causal. La meningitis bacteriana aguda remarca el origen bacteriano de este síndrome, el cual se caracteriza por el inicio agudo de sus síntomas y pleocitosis de predominio neutrofílico. Cada uno de los agentes bacterianos, parasíticos o fúngicos terminan por categorizar las diferentes presentaciones de este cuadro clínico (Ej., meningitis meningocóccica, meningitis criptocóccica. Es en este grupo en específico de etiología en el cual se basara el siguiente artículo. Por último pero no menos importante tenemos la meningitis aséptica, denominada de esta forma debido a una respuesta celular no pirógena causada por muchos tipos de agentes. Los pacientes muestran un inicio agudo de síntomas meníngeos, fiebre y pleocitosis pero de predominio linfocítico. Después de análisis especializados, se da pro concluido que la mayoría de los agentes

  8. Efecto de la adición de materia orgánica sobre la dinámica poblacional bacteriana del suelo en cultivos de papa y maíz

    Directory of Open Access Journals (Sweden)

    David García Ventocilla

    2012-02-01

    Full Text Available Se determinó el efecto de la fertilización orgánica sobre las poblaciones bacterianas del suelo en cultivos de papa y maíz durante la campaña agrícola 2008-2009 en terrenos de cuatro localidades del Valle del Mantaro: INIA Santa Ana (Huancayo, en la EEA El Mantaro (Jauja, Vista Alegre y Huayao (ambos en Chupaca. En estos lugares se instalaron parcelas experimentales de papa (Solanum tuberosum L. Var. Canchan y maíz (Zea maíz L. Var. Cusco mejorado bajo abonamiento orgánico (vacuno, ovino, cuy, fertilización química y sin fertilización alguna (testigo. Para dicho efecto se empleó las técnicas de la Electroforesis en Gel de Gradiente Desnaturalizante (DGGE con amplificación de la región 968 – 1401 del rDNA 16S. Los resultados obtenidos muestran que la variabilidad de las poblaciones bacterianas en los suelos está afectado directamente por el tipo de cultivo mas no por el tipo de fertilización ya que el efecto de este último resulta variable para cada zona experimental y cultivo encontrándose solo en la zona experimental de Chupaca - Maíz una segregación de los tratamientos con fertilización orgánica de los tratamientos químicos. También se ha encontrado que la variación de las comunidades microbianas no sufre variaciones significativas en los suelos con cultivos de maíz obteniéndose coeficientes de similaridad para todos los tratamientos por encima del 80% mientras que para los tratamientos en los cultivos de papa dicho coeficiente fue de tan solo del 60%.

  9. Evaluación de una solución inmovilizadora para criopreservación del semen de Colossoma macropomum, “Gamitana”

    Directory of Open Access Journals (Sweden)

    Ehrlich Llasaca-Calizaya

    2015-01-01

    Full Text Available El objetivo del trabajo fue la criopreservacion de semen, que permitirá constituir un banco genético, para lo cual se buscó obtener una solución  inactivadora de colecta para el semen de Colossoma macropomum  “gamitana”, que permita obtener espermatozoides, con buena motilidad de activación después de la descongelación, en nitrógeno líquido. Se utilizó semen de reproductores mantenidos, del Instituto de Investigaciones de la Amazonía Peruana (IIAP inducidos con Conceptal® y sin inducir mantenidos en el Centro de Acuicultura Nuevo Horizonte del Fondo Nacional de Desarrollo Pesquero (CANH - FONDEPES. El semen fue colectado en soluciones inactivadoras de 9% y 10% de NaCl, añadiendo 2 g/L, 4 g/L y 8 g/L de NaHCO3,  soluciones de sacarosa (300 mM, 400 mM y 500 mM sola o con 1,5 g/L, 1 g/L y 0,5 g/Lde NaCl. Se concluye que el tratamiento de 400 mM de sacarosa dio el mejor resultado, con una motilidad del 80% y 40 segundos de duración. También se evaluó la motilidad, después de una hora de almacenamiento a temperatura ambiente, con 60% de motilidad después de la activación y 20 segundos de duración. Este trabajo permitirá desarrollar  un protocolo de criopreservación para lotes de semen inmovilizados, con tiempo suficiente para preparar las pajuelas, congelarlas en nitrógeno líquido y optimizar el manejo de reproductores.

  10. Evaluación de una solución inmovilizadora para criopreservación del semen de Colossoma macropomum, “Gamitana”

    Directory of Open Access Journals (Sweden)

    Ehrlich Llasaca Calizaya

    2014-12-01

    Full Text Available El objetivo del trabajo fue la criopreservacion de semen, que permitirá constituir un banco genético, para lo cual se buscó obtener una solución inactivadora de colecta para el semen de Colossoma macropomum  “gamitana”, que permita obtener espermatozoides, con buena motilidad de activación después de la descongelación, en nitrógeno líquido. Se utilizó semen de reproductores mantenidos, del Instituto de Investigaciones de la Amazonía Peruana (IIAP inducidos con Conceptal® y sin inducir mantenidos en el Centro de Acuicultura Nuevo Horizonte del Fondo Nacional de Desarrollo Pesquero (CANH - FONDEPES. El semen fue colectado en soluciones inactivadoras de 9% y 10% de NaCl, añadiendo 2 g/L, 4 g/L y 8 g/L de NaHCO3, soluciones de sacarosa (300 mM, 400 mM y 500 mM sola o con 1,5 g/L, 1 g/L y 0,5 g/Lde NaCl. Se concluye que el tratamiento de 400 mM de sacarosa dio el mejor resultado, con una motilidad del 80% y 40 segundos de duración. También se evaluó la motilidad, después de una hora de almacenamiento a temperatura ambiente, con 60% de motilidad después de la activación y 20 segundos de duración. Este trabajo permitirá desarrollar un protocolo de criopreservación para lotes de semen inmovilizados, con tiempo suficiente para preparar las pajuelas, congelarlas en nitrógeno líquido y optimizar el manejo de reproductores.

  11. Efecto del método de obtención de semen de ovino sobre la calidad espermática (Effect of the method of obtaining of ovine semen on the spermatic quality

    Directory of Open Access Journals (Sweden)

    Córdova-Izquierdo, Alejandro1*; ; ; y

    2006-08-01

    Full Text Available La colecta del semen del ganado ovino, ayuda a identificar problemas relacionados con el desempeño reproductivo de estos animales. Se han utilizado diferentes pruebas laboratoriales, para valorar la calidad seminal de los ovinos y poder realizar predicciones indirectas del potencial reproductivo de los machos reproductores; no obstante, su naturaleza subjetiva, no asegura el poder de fecundación de los machos. El objetivo de este trabajo fue valorar el efecto del método de obtención del semen de ovinos sobre la calidad espermática. Se empelaron 10 machos ovinos de la raza Suffolk de una Unidad de Producción Ovina, ubicada en el Estado de México, México. Para la recolección seminal se utilizó vagina artificial y directamente de la vagina, después de la monta natural. El semen fue valorado macro y microscópicamente. Los resultados promedios fueron, para vagina artificial: volumen 1.11 ml, pH 6.62, motilidad 64.5 %, concentración de espermatozoides/ml 206.4 x106, vivos 68.5 %, Muertos 31.87% y anormalidades 23.96%; directamente de la vagina: volumen 0.57 ml, pH 6.75, motilidad 60.4%, concentración de espermatozoides/ml 176.02 x 106, vivos 62.62 %, muertos 37.27% y anormalidades 11.12%. Los resultados indican que los resultados obtenidos con vagina artificial fueron los mejores. The collection of the semen of the livestock ovine, helps to identify problems related with the reproductive acting of these animals. They have been used different you prove laboratories, to value the seminal quality of the ovines and power to carry out indirect predictions of the reproductive potential of the reproductive males; nevertheless, its subjective nature, doesn't assure the power of fecundation of the males. The objective of this work was to value the effect of the method of obtaining of the ovines semen on the spermatic quality. You empelaron 10 male ovines of the race Suffolk of a Unit of Production Ovine, located in the State of Mexico, Mexico

  12. Estrategia para la mejora de la degradación del ácido p-cumárico mediante selección bacteriana

    OpenAIRE

    Otal, E.; Arnáiz Franco, Carmen; Gutiérrez, J.C.; Lebrato Martínez, Julián

    2003-01-01

    Artículo original escaneado para el Repositorio (archivo delegado) Recientemente está recibiendo gran aceptación el tratamiento integrado quimicobiológico de aguas residuales que contienen compuestos orgánicos defícilmente biodegradables. Sim embargo, estudios realizados con aguas sintéticas con ácido p-cumárico mostraron que el tratamiento químico previo al tratamiento biológico no mejoraba su degradación. En este trabajo se han aislado e identificado estirpes bacterianas capaces de crece...

  13. Role of intestinal bacterial overgrowth and intestinal motility in bacterial translocation in experimental cirrhosis Papel del sobrecrecimiento bacteriano intestinal y de la motilidad intestinal en la traslocación bacteriana en un modelo experimental de cirrosis

    Directory of Open Access Journals (Sweden)

    E. Sánchez

    2005-11-01

    Full Text Available Background: intestinal bacterial overgrowth (IBO is related to small bowel motility and has been involved in the pathogenesis of bacterial translocation (BT in experimental models, and both overgrowing gut flora and translocating bacteria to mesenteric lymph nodes are common features in cirrhosis. Objectives: the aims of this study were to analyze cecal aerobic bacteria and intestinal transit in cirrhotic rats, and their relationship with BT, evaluating the role of intestinal bacterial overgrowth and small bowel dismotility in the development of BT in experimental cirrhosis. Material and methods: we included twenty-seven male Sprague-Dawley rats with carbon tetrachloride-induced cirrhosis without ascites and ten controls. Cultures of mesenteric lymph nodes (MLN, peripheral and portal blood, liver, spleen and cecal samples were carried out. Small intestinal transit was determined in ten cirrhotic rats and in ten control rats. Results: the prevalence of bacterial translocation was 56%. Total cecal aerobic bacteria count was significantly higher in cirrhotic rats than in control rats (p Introducción: el sobrecrecimiento bacteriano intestinal (SBI está relacionado con la motilidad del intestino delgado y diferentes trabajos con modelos experimentales han sugerido su relación con el desarrollo de traslocación bacteriana (TB. Tanto el sobrecrecimiento bacteriano intestinal como la traslocación bacteriana son eventos frecuentes en la cirrosis hepática. Objetivos: los objetivos de este estudio han sido analizar la población cecal de bacterias aerobias y el tránsito intestinal en un modelo de ratas cirróticas y su relación con la TB. Material y métodos: el estudio se ha realizado en un modelo experimental de cirrosis inducida por tetracloruro de carbono por vía oral en ratas Sprague-Dawley. Se llevaron a cabo cultivos microbiológicos convencionales a partir de ganglios linfáticos mesentéricos (GLM, sangre portal y periférica, h

  14. Aplicación de visualización de una ontología para el dominio del análisis del semen humano Application to visualize an ontology for the human semen analysis domain

    Directory of Open Access Journals (Sweden)

    Roberto Casañas

    2007-06-01

    Full Text Available En este trabajo se presenta el diseño e implementación de una ontología para el dominio del análisis del semen humano, cuyo objetivo es representar, organizar, formalizar y estandarizar el conocimiento del dominio, para que éste pueda ser compartido y reutilizado por distintos grupos de personas y aplicaciones de software. Para visualizar la ontología se desarrolló una aplicación basada en una arquitectura cliente/servidor para ambientes Web, la cual está constituida por un módulo de Administración y otro de Acceso Público. A través del primero se mantiene el sitio Web de la ontología, mientras que el segundo permite a los usuarios acceder al conocimiento almacenado y a un conjunto de recursos tales como imágenes, videos, artículos relativos al dominio, manuales y protocolos de laboratorio. La arquitectura propuesta facilita la observación y recuperación de las complejas estructuras de conocimiento, así como la navegación y administración de la información representada en la ontología. El enfoque utilizado en el diseño de los mecanismos de recuperación de información está dirigido tanto a usuarios poco familiarizados con el vocabulario del dominio, como a aquellos que ya lo conocen. Esta funcionalidad es de especial interés dado lo heterogénea que resulta la audiencia a la que está dirigida la ontología, como son profesionales y estudiantes de las ciencias de la salud, entre otros. La metodología Methontology fue seleccionada para desarrollar la ontología y se utilizó el editor Protégé para su implementación.The following work presents the design and implementation of an ontology for human semen analysis whose objective is to present, organize, formalize and standardize the domain knowledge, in order to be shared and reused by different groups of people and software applications. To visualize this ontology, a Web application based on a client/server architecture was developed, which is constituted by an

  15. Las enfermedades periodontales como infecciones bacterianas Periodontal diseases as bacterial infection

    OpenAIRE

    2005-01-01

    Las infecciones periodontales son un conjunto de enfermedades localizadas en las encías y estructuras de soporte del diente. Están producidas por ciertas bacterias provenientes de la placa bacteriana. Estas bacterias son esenciales para el inicio de la enfermedad, pero existen factores predisponentes del hospedador y microbianos que influyen en la patogénesis de la enfermedad. La microbiota bacteriana periodontopatógena es necesaria pero no suficiente para que exista enfermedad, siendo necesa...

  16. Efecto de la manipulación del semen criopreservado de bovinos Bos Taurus sobre la integridad espermática

    OpenAIRE

    Norberto Villa-Duque; Claudia Marcela Amaya-Torres; Darwin García-Rojas; Natalia Nieto-Omeara; Natalia Terán-Acuña

    2016-01-01

    En el estudio se evaluó el efecto de descongelar y aplicar semen de bovinos Bos Taurus en 33 ganaderías del Magdalena Medio colombiano, y se estudió in vitro el efecto de la injuria encontrada sobre la integridad de las membranas espermáticas. La información en fincas se recopiló mediante formulario específico, mientras que el estudio in vitro se ejecutó en el laboratorio de Biotecnología Reproductiva Animal del Instituto Universitario de la Paz (Barrancabermeja, Santander). El estudio consis...

  17. Conservación del semen bovino con 2-bromo-5-(2-bromo-2-nitrovinil-Furano (Bovine semen conservation with 2-bromo-5-(2-bromo-2-nitrovinyl-furan

    Directory of Open Access Journals (Sweden)

    Enrique A. Silveira Prado

    2005-11-01

    Full Text Available Se evaluó la eficacia del 2-bromo-5-(2-bromo-2-nitrovinil-furano (denominado G-1 ¾ingrediente farmacéutico activo de acción bactericida de amplio espectro y fungicida¾, como sustituto de los antibióticos comúnmente utilizados en la preservación del semen bovino. Con este objetivo cada uno de 20 eyaculados se dividió en tres alícuotas (grupos A, B y C; al grupo A se le adicionó penicilina (1000 UI/mL y estreptomicina (500 mg/mL y a los grupos B y C concentraciones del G-1 de 5 y 10 mg/mL respectivamente. A las 24 horas post congelación se descongelaron pas­tillas de cada grupo y se evaluaron respecto a las características físicas y calidad higiénico-sanitaria según se encuentra establecido. En las condiciones del ensayo no se detectaron diferencias estadísticamente significativas en los parámetros de calidad evaluados entre los grupos A y C, por lo que se concluyó que el G-1 en concentraciones de 10 mg/mL posee una eficacia semejante a los antimicrobianos utilizados comúnmente con este fin. The effectiveness of 2-bromo-5-(2-bromo-2-nitrovinyl-furan (denominated G-1 ¾on active pharmaceutical ingredient having broad spectrum bactericide and fungicide activity¾ as a substitute of antibiotics commonly used to preserve bovine semen was evaluated. From this purpose each one of 20 ejaculations was divided into three aliquots (groups A, B and C. Penicillin (1000 IU/mL and streptomycin (500 mg/mL was added to group A and G-1 at a concentrations of 5 and 10 mg/mL to groups B and C respectively. Pills were defrosted 24 hours post freezing and their physical characteristics and hygienic-sanitary qualities were evaluated according to standard procedures. There were no statistically significant differences in the parameters evaluated between groups A and C. It was concluded that G-1 at a concentration of 10 mg/mL has an effectiveness similar to that of antimicrobials commonly used to preserve semen.

  18. Elaboración del video educativo sobre la identificación bacteriana por el método de gram

    OpenAIRE

    Medina, Jose

    2009-01-01

    El presente video fue creado y diseñado con la finalidad de proporcionar un instrumento didáctico para aprender de una manera eficaz sobre la identificación bacteriana empleando el método de tinción de Gram. La tinción de Gram es uno de los métodos más utilizados en el laboratorio bacteriológico, por lo cual es necesario llegar a un dominio adecuado de su manipulación. Este video tiene una duración de 8 minutos, durante los cuales se podrá adquirir el conocimiento suficiente para entender el ...

  19. Las enfermedades periodontales como infecciones bacterianas

    Directory of Open Access Journals (Sweden)

    A. Bascones Martínez

    Full Text Available Las infecciones periodontales son un conjunto de enfermedades localizadas en las encías y estructuras de soporte del diente. Están producidas por ciertas bacterias provenientes de la placa bacteriana. Estas bacterias son esenciales para el inicio de la enfermedad, pero existen factores predisponentes del hospedador y microbianos que influyen en la patogénesis de la enfermedad. La microbiota bacteriana periodontopatógena es necesaria pero no suficiente para que exista enfermedad, siendo necesaria la presencia de un hospedador susceptible. Estas enfermedades se han clasificado en gingivitis, limitadas a las encías y periodontitis, extendidas a tejidos más profundos. La clasificación de las enfermedades periodontales ha ido variando a lo largo de los años y es en el International Workshop for a Clasification of Periodontal Diseases and Conditions, en 1999, cuando se aprueba la clasificación que se expone en este trabajo. En él, se hace una revisión global de los diferentes cuadros de las enfermedades periodontales. Posteriormente, se propone el empleo de antibioterapia de utilización sistémica como la amoxicilina, amoxicilina-clavulánico y metronidazol como primera opción de tratamiento coadyuvante de estas enfermedades.

  20. Centrifugación coloidal del semen crioconservado del perro para la selección de espermatozoides

    OpenAIRE

    Alcaraz Aranda, Lucrecia

    2014-01-01

    La crioconservación del esperma es un pilar básico en los programas de reproducción asistida en la especie canina, al eliminar ésta los inconvenientes del transporte de animales, permitir la reproducción de perros genéticamente superiores en aquellos casos en los que el apareamiento natural resulta imposible y al ofertar la posibilidad de almacenar el esperma indefinidamente para su posterior uso. Pese a todas estas ventajas, la fertilidad potencial del esperma congelado se ve ...

  1. Evaluación de la resistencia bacteriana frente a tres antibióticos usados en la maduración del camarón marino (Litopenaeus vannamei).

    OpenAIRE

    Luna G., Diana; De León L., Jorge; Vallejo I., Adriana; Velásquez L., Gustavo

    2003-01-01

    La incidencia de las enfermedades bacteriana en camaronicultura, ha conducido a la utilización de terapéuticos, con el subsecuente problema de generación de resistencia a mediano y largo plazo, afectando la producción. Con el objetivo de evaluar la resistencia bacteriana en un sistema de maduración de Litopenaeus vannamei , fueron aisladas 18 cepas bacterianas correspondientes a V. parahaemolyticus (n=3), V. alginolyiticus (n=5), V. hollisae (n=5) y Flavobacterium spp. (n=5). ...

  2. Revisão sistemática do uso da dexametasona como terapia adjuvante na meningite bacteriana em crianças Revisión sistemática del uso de la dexametasona como terapia adyuvante en la meningitis bacteriana en niños Systematic review of dexamethasone as an adjuvant therapy for bacterial meningitis in children

    OpenAIRE

    2012-01-01

    OBJETIVO: Analisar a melhor evidência disponível nos últimos 15 anos com relação aos benefícios da terapia adjuvante com dexametasona na meningite bacteriana em população pediátrica. FONTES DE DADOS: Das bases de dados Medline, Lilacs e SciELO, foram analisados ensaios clínicos randomizados de 1996 a 2011, os quais comparavam a dexametasona ao placebo e/ou a outra terapia adjuvante em pacientes com meningite bacteriana diagnosticada laboratorialmente por critérios quimiocitológicos e/ou bacte...

  3. Efectividad del cepillo iónico basado en dióxido de titanio en comparación con el cepillo vitis junior en la eliminación de placa bacteriana en niños de 8 a 10 años del Colegio Weberbauer Schule

    OpenAIRE

    Conte Pujalt, Giacomo; Olivares Espinoza, Jimmy

    2011-01-01

    Objetivo. Determinar la efectividad del cepillo iónico basado en dióxido de titanio comparándolo con el cepillo Vitis Junior en la remoción de placa bacteriana en niños de 8 a 10 años del colegio Weberbauer Schule. Material y método. El estudio se realizó en un grupo de 30 niños, los cuales se comportaron como grupo control y experimental, ya que se trabajó con ellos en dos oportunidades diferentes. En la primera se realizó el cepillado con el cepillo Vitis Junior mediante la técnica...

  4. IDENTIFICACIÓN MOLECULAR DE POBLACIONES BACTERIANAS ASOCIADAS AL CARACOL PALA (Strombus gigas DEL CARIBE COLOMBIANO Molecular Identification Of Bacterial Populations Associated To Queen Conch (Strombus gigas From Colombian Caribbe

    Directory of Open Access Journals (Sweden)

    EDINSON ANDRÉS ACOSTA

    Full Text Available El caracol Pala, Strombus gigas (Strombidae, es de gran importancia ecológica y socioeconómica en el área caribeña colombiana. Sin embargo, es una especie catalogada como "vulnerable" y existe muy poca información referente a las especies bacterianas asociadas al caracol que puedan ser importantes para el desarrollo, manejo productivo y de seguridad acuícola de estos gastrópodos. En este trabajo, nosotros empleamos un estudio microbiológico y molecular de la región intergénica entre los genes 16S y 23S rDNA, análisis del gen rDNA 16S y secuenciación, para analizar las bacterias asociadas al caracol Pala (S. gigas. La composición de bacterias cultivables asociadas fue evaluada por su capacidad para crecer en agar marino y en medios de cultivos selectivos. De un total de 28 muestras analizadas encontramos que el número de bacterias cultivadas en condiciones aerobias fue de alrededor 10(6 ufc mL-1 donde las bacterias pertenecientes a la familia Vibrionacea fueron las más abundantes, cerca de >10(5 ufc mL-1. El análisis molecular de la región intergénica entre los genes 16S y 23S rDNA de las diferentes muestras, reveló una gran complejidad bacteriana asociada a S. gigas. Las secuencias de los amplificados del gen rDNA 16S identificó Pseudoalteromonas sp., Halomonas sp., Psycrobacter sp., Cobetia sp., Pseudomonas sp. y Vibrios sp. Nuestros resultados podrían sugerir un rol importante de estas bacterias como componentes de la comunidad asociada al S. gigas. Esta información puede complementar los estudios que se están implementando en los procesos para la conservación y repoblamiento de las poblaciones de S. gigas en Colombia.The Queen Conch, Strombus gigas (Strombidae, is a species of great ecological and socioeconomic importance in the Caribbean area of Colombia. However, it is currently catalogued as "vulnerable"; there is limited information concerning the bacterial species associated with conch and important in the

  5. Aplicación de la celulosa bacteriana a la restauración del patrimonio bibliográfico y documental en papel

    OpenAIRE

    Santos de Dios, Sara M.

    2015-01-01

    La preservación del patrimonio bibliográfico y documental en papel es uno de los mayores retos a los que se enfrentan bibliotecas y archivos de todo el mundo. La búsqueda de soluciones al problema del papel degradado ha sido abordada históricamente desde dos líneas de trabajo predominantes: la conservación de estos documentos mediante la neutralización de los ácidos presentes en ellos con agentes alcalinos, y su restauración mediante el método de laminación fundamentalmente con papel de orige...

  6. La placa bacteriana: conceptos básicos para el higienista bucodental

    OpenAIRE

    Poyato Ferrera, Manuel María; Segura-Egea, Juan J.; Ríos-Santos, J.V.; Bullon, Pedro

    2001-01-01

    La placa bacteriana es el factor etiológico principal de las dos enfermedades bucodentales de mayor prevalencia, la caries y la enfermedad periodontal. El higienista bucodental y colaborador del odontoestomatólogo en la aplicación del programa de control de placa, debe conocer en profundidad la microbiología de la placa bacteriana dental, así como los mecanismos implicados en su patogenicidad cariogénica y periodontal, aspectos que se revisan a continuación. Dental plaque is the main etiol...

  7. Influencia del tabaquismo en la resistencia bacteriana después de la profilaxis frente a infecciones urinarias recurrentes con antibiótico o con vacuna

    OpenAIRE

    Santos Antunes, María Tatiana

    2014-01-01

    [ES]La infección del tracto urinario (ITU) es la segunda infección más frecuente del ser humano después de las respiratorias. En las mujeres, el riesgo de padecer una ITU a lo largo de su vida es superior al 50%. Hata el 20% de mujeres jóvenes con una primera ITU tendrán una infección recurrente. Con cada ITU se incrementa el riesgo de padecer una nueva ITU. El tabaco es una de las mayores amenazas para la salud pública que ha tenido que afrontar nunca el mundo.Mata a casi 6 millones de pe...

  8. Inoculación bacteriana en el crecimiento y calidad del fruto de cinco variedades de fresa en suelos con pH contrastante

    OpenAIRE

    2016-01-01

    En un primer experimento se determinó el crecimiento de cinco variedades de fresa en dos suelos con pH contrastante (4.4 y 8.8). La producción de materia seca de la parte aérea, área foliar, volumen radical y concentración de N, P, K en la parte aérea se determinaron 125 días después del trasplante (ddt). Las plantas de todas las variedades cultivadas en pH 4.4 superaron a las cultivadas en pH 8.8 en todas las variables. Las variedades CP-06-15 y Festival fueron las que mejor se adaptaron a l...

  9. La lectura interpretativa del antibiograma: Una herramienta para predecir la resistencia bacteriana en el laboratorio de microbiología de rutina.

    Directory of Open Access Journals (Sweden)

    María del Pilar Crespo

    2009-11-01

    Full Text Available La aparición cada vez más frecuente y diversa de los mecanismos de resistencia a nivel microbiano y sobre todo en aquellas bacterias patógenas facultativas e incluso oportunistas, ha traído consecuencias importantes en términos de morbilidad y mortalidad y millonarias pérdidas no sólo humanas sino económicas. El impacto de la diseminación de estas cepas escapa a los cálculos establecidos y en la mayoría de los casos no se ha dado la relevancia real y pertinente al problema que se afronta. Como es tal vez menos complicado evitar que tratar en este caso específico, es de suma importancia poder reconocer, descubrir, tratar eficazmente y prevenir las infecciones por microorganismos resistentes. Esta revisión tiene como objeto profundizar en el cómo y para qué de una herramienta que puede utilizarse para este efecto en el laboratorio de microbiología de rutina: “la lectura interpretativa del antibiograma”. Esta puede ser aplicada en las bacterias más frecuentes y constituye una herramienta sencilla y accesible que permite hacer inferencias sobre los mecanismos de resistencia más estudiados. Lo anterior representa un importante aporte para un mejor y mayor enfoque en el tratamiento antibiótico del paciente infectado con estas cepas y a la vez suministra un control de calidad al informe y diagnóstico microbiológico.

  10. A resistência bacteriana no contexto da infecção hospitalar

    OpenAIRE

    Neusa Santos de Queiroz

    2004-01-01

    Este artículo aporta algunos aspectos sobre el impacto de la resistencia bacteriana en el contexto de la infección hospitalar. Alerta sobre el papel importante de los profissionais de la salud para el control de la infección hospitalar y del grave problema del uso indiscriminado de los antibióticos que han generado una presión selectiva sobre las bacterias del ambiente hospitalario tornándolas multiresistentes

  11. A resistência bacteriana no contexto da infecção hospitalar

    Directory of Open Access Journals (Sweden)

    Neusa Santos de Queiroz

    2004-01-01

    Full Text Available Este artículo aporta algunos aspectos sobre el impacto de la resistencia bacteriana en el contexto de la infección hospitalar. Alerta sobre el papel importante de los profissionais de la salud para el control de la infección hospitalar y del grave problema del uso indiscriminado de los antibióticos que han generado una presión selectiva sobre las bacterias del ambiente hospitalario tornándolas multiresistentes

  12. Resistencia bacteriana Bacterial resistance to antimicrobial agents

    Directory of Open Access Journals (Sweden)

    Jesualdo Fuentes

    1993-01-01

    Full Text Available

    Se presenta un panorama de la resistencia bacteriana incluyendo su fisiopatogenia y formas de presentación y se establecen algunas consideraciones generales de tipo clínico como auxiliares para racionalizar el uso de los antimicrobianos y evitar o retardar el problema de la resistencia; éste plantea la necesidad de un reordenamiento definitivo en la prescripción de antimicrobianos. No será tanto la creación o descubrimiento de nuevos antibióticos sino la racionalización del manejo de los existentes lo que permitirá alcanzar victorias sobre estos microorganismos. Es Importante mantener educación continua sobre el uso adecuado de los antimicrobianos desde los puntos de vista epidemiológico, farmacocinético y fisiopatogénico.

    An overview on bacterial resistance to antimicrobial agents is presented. It includes the different genetic mechanisms for Its development and the biochemical phenomena that explain It. Some clinical considerations are proposed in order to rationalize the use of these drugs and to avoid or delay the appearance of resistance.

  13. Cumplimiento de las recomendaciones internacionales en la lucha contra las resistencias bacterianas

    DEFF Research Database (Denmark)

    Malo, Sara; Jose Rabanaque, Mara; Bjerrum, Lars

    2016-01-01

    Introducción: El aumento de la resistencia a antibióticosrepresenta una amenaza para la salud pública al poner en riesgoel tratamiento futuro de las infecciones bacterianas. Este estudiotiene como objetivo describir el cumplimiento de las recomendaciones del Advisory Group on Integrated Surveilla...

  14. Revisão sistemática do uso da dexametasona como terapia adjuvante na meningite bacteriana em crianças Revisión sistemática del uso de la dexametasona como terapia adyuvante en la meningitis bacteriana en niños Systematic review of dexamethasone as an adjuvant therapy for bacterial meningitis in children

    Directory of Open Access Journals (Sweden)

    João Antonio G. G. Prats

    2012-12-01

    Full Text Available OBJETIVO: Analisar a melhor evidência disponível nos últimos 15 anos com relação aos benefícios da terapia adjuvante com dexametasona na meningite bacteriana em população pediátrica. FONTES DE DADOS: Das bases de dados Medline, Lilacs e SciELO, foram analisados ensaios clínicos randomizados de 1996 a 2011, os quais comparavam a dexametasona ao placebo e/ou a outra terapia adjuvante em pacientes com meningite bacteriana diagnosticada laboratorialmente por critérios quimiocitológicos e/ou bacteriológicos, na faixa etária de 29 dias aos 18 anos. Os desfechos avaliados foram mortalidade e ocorrência de sequelas neurológicas e/ou auditivas. Foram excluídos estudos relacionados à meningite tuberculosa. SÍNTESE DOS DADOS: Com os critérios utilizados, foram identificadas cinco publicações correspondentes a quatro protocolos de estudo. Nenhum dos estudos mostrou diferenças entre a dexametasona e o placebo para os desfechos avaliados. Os estudos analisados tiveram alta qualidade (escore de Jadad et al=5. CONCLUSÕES: As evidências encontradas na literatura são insuficientes para indicar de forma rotineira o uso da dexametasona como terapia adjuvante para redução de mortalidade, perda auditiva e sequelas neurológicas em pacientes pediátricos com meningite bacteriana não tuberculosa.OBJETIVO: El presente estudio tiene por objetivo el análisis de la mejor evidencia disponible los últimos 15 años respecto a los beneficios de la terapia adyuvante con dexametasona en la meningitis bacteriana en población pediátrica por medio de revisión sistemática. FUENTES DE DATOS: De las bases de datos Medline, Lilacs y ScieLO, se analizaron ensayos clínicos aleatorios de 1996 a 2011 que comparaban la dexametasona al placebo y/u otra terapia adyuvante, en pacientes con meningitis bacteriana diagnosticada laboratorialmente por criterios quimiocitológicos y/o bacteriológicos, en la franja de edad de 29 días a 18 años. Los desenlaces

  15. Antibióticos vs. resistencia bacteriana

    Directory of Open Access Journals (Sweden)

    Elso Manuel Cruz Cruz

    2015-02-01

    Full Text Available La resistencia bacteriana a los antibióticos es un problema de salud mundial que se encuentra en constante evolución. De manera frecuente se reportan nuevos mecanismos de resistencia bacteriana a los antibióticos, tanto en bacterias gram negativas como en gram positivas. La presencia de resistencia en una bacteria causante de infección disminuye las posibilidades de obtener la curación clínica y la erradicación bacteriológica e incrementa los costos del tratamiento, la morbilidad y la mortalidad; por lo que es importante seleccionar el tratamiento adecuado. Uno de los aspectos que más preocupa a los médicos en el diagnóstico y tratamiento de una infección es la llamada resistencia adquirida, la cual ocurre en una bacteria inicialmente sensible a los antibióticos, por cambios, mutaciones o la adquisición de genes de resistencia durante el fenómeno de transferencia genética lateral, proceso por medio del cual un organismo transfiere material genético a otra célula que no es descendiente. La resistencia bacteriana adquirida a los antibióticos puede ser de distintos tipos, dependiendo de la presión selectiva, las mutaciones o la transferencia de genes de resistencia. Las definiciones de resistencia se clasifican según el número y clase de antibióticos afectados. La multirresistencia (Multiple Drug Resistance, MDR se define como la ausencia de sensibilidad a, por lo menos, un fármaco en tres o más de las categorías de antibióticos; la resistencia extrema (Extensively Drug-Resistant, XDR se refiere a la ausencia de sensibilidad a, por lo menos, un agente en todas las categorías de antimicrobianos, excepto en dos de ellas o menos, y la resistencia a todos los antimicrobianos se define como resistencia a todas las categorías de antibióticos. (1 Los mecanismos de resistencia dependen del tipo de bacteria que los desarrollen. Las bacterias gram positivas que producen con más frecuencias infecciones en humanos y que por

  16. GENERACION DE UN BANCO DE SEMEN DE CABALLOS FINA SANGRE DE TIRO PESADO, A PARTIR DEL DISEÑO DE PROTOCOLOS DE CRIOPRESERVACION PARA USO EN INSEMINACION ARTIFICIAL (IA) EN EL PLAN NACIONAL DE FOMENTO EQUINO (PNFE).

    OpenAIRE

    RAMIREZ REVECO, ALFREDO RAUL

    2008-01-01

    Este proyecto se gestó y ejecutó con el fin de apoyar el Plan Nacional de Fomento Equino (PNFE) para la agricultura, a través del estudio y la inclusión de la Inseminación Artificial (IA) con semen equino congelado y refrigerado. El PNFE nace hace tres décadas con el objetivo de mejorar la genética caballar de tiro en Chile a través de cruzamientos absorbentes ejecutados mediante monta dirigida de sementales de tiro pesado del Ejercito de Chile sobre yeguas mestizas de los beneficiarios. En e...

  17. Cambios en el espectro de la meningitis bacteriana espontánea en el paciente adulto en un hospital de tercer nivel

    OpenAIRE

    Pomar Solchaga, Virginia

    2014-01-01

    La tesis titulada "Cambios en el espectro de la meningitis bacteriana espontánea en el paciente adulto en un hospital de tercer nivel" está formada por 3 artículos y tiene como objetivo global analizar los cambios observados en las meningitis bacterianas del adulto en las últimas décadas en un hospital de tercer nivel de Barcelona. En el primer artículo, se evalúan 635 meningitis bacterianas desde 1982 al 2010, y se analizan en 2 períodos: 1982-1995 y 1996-2010. En el segundo periodo la pobla...

  18. Efectividad del uso empírico de vancomicina para tratar la meningitis bacteriana aguda por Streptococcus pneumoniae. Revisión sistemática de la literatura y metaanálisis.

    OpenAIRE

    Galeano Tapias, Adriana Catalina; Piedrahita Vargas, Cristian Alfonso

    2015-01-01

    Introducción. Debido al aumento de la frecuencia de Streptococcus pneumoniae resistente a penicilina en meningitis bacteriana aguda y de los casos de fracaso al tratamiento con betalactámicos, se recomienda el uso combinado de vancomicina con cefalosporinas de tercera generación como primera línea de tratamiento empírico. Sin embargo, la efectividad de dicho régimen y su relación con la resistencia antibiótica no se conoce con claridad. Objetivo. Evaluar la efectividad de la administración em...

  19. HELICOBACTER PYLORI EN LA FLORA BACTERIANA ORAL

    OpenAIRE

    Moromi Nakata, Hilda; Departamento Académico Ciencias Básicas Estomatológicas. Facultad de Odontología. UNMSM.

    2014-01-01

    No hay duda de la relación existente entre enfermedades orales con otras enfermedades sistémicas. En tal contexto, las bacterias de la flora bacteriana oral, que alcanzan alrededor de 350 especies, para la mayoría de tales bacterias no se ha demostrado un rol específico, conociéndose sí una clara relación entre los Estreptococos orales (Streptococcus sanguis, Strecoccus mutans, Streptococcus sobri nus) y Actinobacillus actinomycetencomitans, entre otros, con la endocarditis bacteriana; así co...

  20. Tratamento ambulatorial da endocardite bacteriana estreptocócica Tratamiento clínico de la endocarditis bacteriana estreptocócica Ambulatory treatment of streptococcal bacterial endocarditis

    Directory of Open Access Journals (Sweden)

    Sirio Hassem Sobrinho

    2010-04-01

    Full Text Available A endocardite bacteriana é uma grave doença infecciosa cujo tratamento é tradicionalmente feito com o paciente internado. recebendo medicação intravenosa. A possibilidade de tratamento domiciliar ou ambulatorial. em casos estritamente selecionados. é atraente tanto do ponto de vista social quanto do econômico. Apresentamos o relato de 6 pacientes com diagnóstico de endocardite bacteriana por Streptococcus. tratados parcial ou integralmente em regime ambulatorial. Todos evoluíram sem complicações e com resolução completa do quadro infeccioso.La endocarditis bacteriana es una severa enfermedad infecciosa cuyo tratamiento se hace tradicionalmente con el paciente internado, recibiendo medicación intravenosa. La posibilidad de tratamiento domiciliar o clínico, en casos estrictamente seleccionados, es atractivo desde el punto de vista social como del económico. Presentamos el caso clínico de 6 pacientes con diagnóstico de endocarditis bacteriana por streptococcus, tratados parcial o integralmente en régimen ambulatorio. Todos evolucionaron sin complicaciones y con resolución completa del cuadro infeccioso.Bacterial endocarditis is a severe infectious disease. of which treatment is traditionally carried out in hospitalized patients through intravenous medication. The possibility of at-home or ambulatory treatment. for stringently selected cases. is attractive from the social as well as from the economic point of view. We report 6 patients with a diagnosis of bacterial endocarditis caused by Streptococcus. treated partially or completely on an outpatient basis. All of them evolved without complications and presented complete resolution of the infection.

  1. Blood in the semen

    Science.gov (United States)

    ... semen URL of this page: //medlineplus.gov/ency/article/003163.htm Blood in the semen To use ... and look for signs of: Discharge from the urethra Enlarged or tender ... Saunders; 2016:chap 129. Small EJ. Prostate cancer. In: Goldman L, Schafer AI, eds. Goldman's Cecil ...

  2. Optimización de las técnicas de acondicionamiento del semen equino para los procesos de conservación seminal

    OpenAIRE

    Gutiérrez Cepeda, Luna

    2013-01-01

    Desde hace muchos años en las técnicas de reproducción asistida en medicina humana existen protocolos de procesamiento de semen para incrementar su calidad. En la industria reproductiva equina, las técnicas de inseminación artificial se han desarrollado ampliamente, aunque el uso de semen criopreservado es menor que en otras especies debido a problemas de supervivencia espermática y variabilidad individual. Esta variabilidad individual también ha sido establecida entre razas. En general, sólo...

  3. Peritonite bacteriana espontânea

    Directory of Open Access Journals (Sweden)

    Strauss Edna

    2003-01-01

    Full Text Available A peritonite bacteriana espontânea ocorre em 30% dos cirróticos com ascite e, neste grupo, apresenta altas taxas de morbidade e mortalidade. Os fatores predisponentes incluem a diminuição da defesa imunológica encontrada no homem nas fases avançadas da cirrose, o supercrescimento da flora intestinal e a translocação bacteriana da luz dos intestinos aos linfonodos mesentéricos. As manifestações clínicas variam de graves a leves ou ausentes, sendo sempre necessária a análise do líquido ascítico. O diagnóstico de peritonite bacteriana espontânea se faz pela contagem de neutrófilos > 250/mm³ no líquido ascítico associado ou não ao crescimento de bactéria na cultura. As enterobactérias predominam como causa da infecção, sendo a Echerichia coli a bactéria mais freqüentemente isolada. O diagnóstico precoce e o tratamento adequado provocaram a queda das taxas de mortalidade nas duas últimas décadas. O uso endovenoso de cefalosporinas de terceira geração mostra-se eficaz em 70% a 95% dos casos. A recorrência de peritonite bacteriana espontânea é comum e pode ser prevenida com norfloxacina oral, de uso contínuo. O surgimento de resistência bacteriana tem estimulado a procura de novas opções para a profilaxia da peritonite bacteriana espontânea; os probióticos constituem nova abordagem promissora, mas que necessita melhor avaliação. Recomenda-se a profilaxia primária de curta duração aos cirróticos com ascite que apresentem episódio de hemorragia digestiva alta.

  4. Congelación y conservación del semen en la especie caprina mediante la utilización de ultracongeladores de -152ºC: Tasa de fertilidad tras inseminación con semen congelado por diferentes protocolos de crioconservación

    OpenAIRE

    Niño González, Tara

    2008-01-01

    [ES] El análisis de los distintos parámetros evaluados para determinar la calidad seminal de siete machos cabríos de raza Majorera, demuestra que la congelación del semen mediante ultracongeladores de -152 ºc es igual de efectiva que la congelación mediante nitrógeno líquido, técnica que se ha venido usando desde hace ya muchos años. se observa cómo la calidad seminal in vitro tras la descongelación no varía en los diferentes períodos de congelación testados (1, 30 y 180 días), así mismo, el ...

  5. Semen quality in Greenland

    OpenAIRE

    Toft, Gunnar; Pedersen, Henning Sloth; Bonde, Jens Peter; research team, INUENDO

    2004-01-01

    Objectives. To quantify semen quality in the Greenlandic population. Study design. A cross-sectional study including recently proven fertile men from four regions including nine municipalities and one settlement in Greenland. Methods. The samples were analysed for sperm cell concentrations and motility using standard methods. Results. In total 201 semen samples were collected. The median sperm cell concentration of fertile men in Greenland was 53 x106 sperm cells/ml, with a median sperm cell ...

  6. Microbiota bacteriana asociada a los cultivos de dos especies de diatomeas bentónicas.

    OpenAIRE

    Hernández-Zulueta, Joicye; Leal, Sylvia; Loza, Sandra

    2014-01-01

    Las diatomeas bentónicas forman biopelículas debido a las sustancias que secretan, que les permite mantenerse adheridas al sustrato. A ellas se asocian bacterias que, dada las condiciones no axénicas de los cultivos, pueden alterar su valor nutricional. El objetivo del presente trabajo fue caracterizar la microbiota bacteriana asociada a los cultivos de las microalgas bentónicas Navicula germanopolonica y Amphora sp., especies utilizadas para la precría del camarón en cultivo. Se aislaron e i...

  7. VULVOVAGINITIS BACTERIANA EN LA PREPÚBER

    OpenAIRE

    Barbosa R.,Gabriel; Sánchez D.,Paola

    2005-01-01

    Considerando la alta incidencia de vulvovaginitis en la consulta de ginecología pediátrica y de adolescentes, y los aspectos únicos de la microbiología y endocrinología de la paciente prepúber se presenta una revisión de la literatura con lo más relevante de los últimos 5 años, en lo que se refiere a epidemiología, etiología, diagnóstico y tratamiento de la vulvovaginitis bacteriana específica de la niña prepúber. No existe claridad acerca de la microflora vaginal normal, lo cual dificulta en...

  8. Adhesión bacteriana a biomateriales Bacterial adhesion to biomaterials

    Directory of Open Access Journals (Sweden)

    C Ábalos

    2005-02-01

    Full Text Available En términos generales, para la adhesión bacteriana, influyen cuatro elementos: Material, Microorganismos, antimicrobianos y mecanismos de defensa. La influencia del material es más importante en los estadios iniciales de la adhesión, pudiendo influir el mismo material, su rugosidad o su energía superficial., si es que existe una influencia del material en la adhesión bacteriana, esta reside en las caracteristicas de la película adquirida y en la especificidad de las proteinas adsorbidas salivares (receptores, que puedan ser condicionadas por la composición del material o por las características de superficie de este.In general terms, there are four elements which influence on bacterial adhesion: the material, the micro organisms, antimicrobials and defence mechanisms. The influence of the material is more relevant at the initial states of adhesion where the proper material, its roughness or its superficial energy can have some influence. If there is some influence of the material in the bacterial adhesion, it relies on the features of the acquired film and on the specificity of the adsorved salivary proteins (receptors, which can be influenced by the composition of the material or the characteristics of its surface.

  9. [Antimutagenic substances in the Armeniacae semen and Persicae semen].

    Science.gov (United States)

    Yamamoto, K; Osaki, Y; Kato, T; Miyazaki, T

    1992-12-01

    Using the Ames/Salmonella/microsome assay, we examined the antimutagenic effect of the hexane extract of Armeniacae semen (apricot (Prunus armeniaca L.) seed), Persicae semen (peach (P. persica Bat.) seed), and seeds of cherry (P. avium L.), plum (P. salicina Lindle) and almond (P. dulcis Mill). Hexane extracts of Armeniacae semen and Persicae semen inhibited the mutagenicity of benzo[a]pyrene (B[a]P), but those of seeds of cherry, plum and almond did not. The mutagenicities of 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2) were also inhibited by the extracts of Armeniacae semen and Persicae semen. Inhibitory substances in Persicae semen were fractionated by silica gel column chromatography and high performance liquid chromatography, and were identified as oleic acid and linoleic acid. The contents of oleic acid and linoleic acid were 0.7 and 0.4% in the hexane extract of Armeniacae semen, and 1.5 and 0.5% in that of Persicae semen, respectively.

  10. Etiología y sensibilidad bacteriana en infección urinaria en niños. Hospital Infantil Club Noel y Hospital Universitario del Valle, Cali, Colombia

    Directory of Open Access Journals (Sweden)

    Consuelo de Rovetto

    2007-06-01

    Full Text Available Introducción: La infección del sistema urinario es la enfermedad renal más común en la edad pediátrica. El aislamiento del germen y el manejo temprano con el medicamento adecuado son prioritarios para evitar cicatrices renales permanentes. Objetivos: Describir los agentes patógenos causantes de infección urinaria y la sensibilidad a los antibióticos y quimioterápicos en niños atendidos en los hospitales Universitario del Valle e Infantil Club Noel en Cali, Colombia.Materiales y métodos: Se llevó a cabo un estudio descriptivo de corte transversal en niños menores de 14 años que recurrieron a los servicios de Urgencias y Consulta Externa de los hospitales Universitario e Infantil Club Noel con sospecha clínica y para-clínica de infección urinaria entre agosto, 2004 y febrero, 2005 a quienes se hizo urocultivo por punción suprapúbica, sonda vesical o micción espontánea. La definición de infección urinaria se basó en el recuento de colonias del urocultivo según el método de recolección. De los urocultivos positivos se tomó el germen y sensibilidad antibiótica. Se excluyeron niños con recuentos de colonias que no cumplían los criterios establecidos, con bacteriuria asintomática, en cateterismo intermitente, inmunocomprometidos, con vesicostomía y hospitalizados en cuidados intensivos.Resultados: De los 154 niños que ingresaron al estudio se excluyeron 31 por recuento de colonias menor al estipulado en el urocultivo. Quedaron para el análisis un total de 123 urocultivos. Se tomó por sonda vesical 50% de los urocultivos, 33 % por micción espontánea y 17% por punción suprapúbica; 58% de los pacientes era de género femenino. El germen que se aisló con más frecuencia (72% fue Escherichia coli, luego Klebsiella (16.4%, Proteus (2.5% y otros tipos de gérmenes (9%. Los antibióticos con sensibilidad mayor de 80% fueron: cefixime, norfloxacina, cefuroxima, cefprozil, ácido nalidíxico, ceftriaxone, amikacina y

  11. Las enfermedades periodontales como infecciones bacterianas Periodontal diseases as bacterial infection

    Directory of Open Access Journals (Sweden)

    A. Bascones Martínez

    2005-12-01

    Full Text Available Las infecciones periodontales son un conjunto de enfermedades localizadas en las encías y estructuras de soporte del diente. Están producidas por ciertas bacterias provenientes de la placa bacteriana. Estas bacterias son esenciales para el inicio de la enfermedad, pero existen factores predisponentes del hospedador y microbianos que influyen en la patogénesis de la enfermedad. La microbiota bacteriana periodontopatógena es necesaria pero no suficiente para que exista enfermedad, siendo necesaria la presencia de un hospedador susceptible. Estas enfermedades se han clasificado en gingivitis, limitadas a las encías y periodontitis, extendidas a tejidos más profundos. La clasificación de las enfermedades periodontales ha ido variando a lo largo de los años y es en el International Workshop for a Clasification of Periodontal Diseases and Conditions, en 1999, cuando se aprueba la clasificación que se expone en este trabajo. En él, se hace una revisión global de los diferentes cuadros de las enfermedades periodontales. Posteriormente, se propone el empleo de antibioterapia de utilización sistémica como la amoxicilina, amoxicilina-clavulánico y metronidazol como primera opción de tratamiento coadyuvante de estas enfermedades.

  12. Biodegradación de HAPs durante la biorremediación aeróbica de suelos contaminados con hidrocarburos del petróleo. Análisis de poblaciones bacterianas y genes funcionales

    OpenAIRE

    Izquierdo Romero, Andrés Ricardo

    2014-01-01

    [spa] La contaminación de suelos con derivados del petróleo es un problema a nivel mundial, especialmente en los países productores y exportadores de crudo, teniendo consecuencias muy perjudiciales para el medio ambiente y la salud humana. Una de las mejores alternativas de remediación de suelos es la aplicación de métodos biológicos, como la biorremediación, por tratarse de una tecnología amigable con el medio ambiente y por sus menores costos en comparación con técnicas físicas y químicas. ...

  13. Biofertilizantes no controle da mancha bacteriana (Xanthomonas spp. do tomateiro

    Directory of Open Access Journals (Sweden)

    Vinícius William Borges Rodrigues

    2016-03-01

    Full Text Available RESUMO A mancha bacteriana do tomateiro, causada por quatro espécies de Xanthomonas pode provocar perdas significativas na produção da cultura e a utilização de biofertilizantes na proteção de plantas tende a reduzir a incidência de doenças. O objetivo do trabalho foi avaliar o efeito dos biofertilizantes no controle preventivo e curativo da mancha bacteriana do tomateiro. Para o controle preventivo da doença, plantas de tomate cultivar Santa Cruz Kada, com 3 a 4 folhas foram pulverizadas com os biofertilizantes (Soil-Set, Agro-Mos e Cop-R-Quick e água (testemunha; e dois dias após foram inoculadas por aspersão com a suspensão bacteriana nas concentrações 109 UFC mL-1 (OD550=0,5 e 106UFC mL-1, com o isolado UFU A35 de Xanthomonas sp. Para o controle curativo, as plantas foram inoculadas com a suspensão bacteriana, e dois dias após foram pulverizadas com os biofertilizantes e água. A severidade da mancha bacteriana foi avaliada usando uma escala diagramática; aos 3, 5, 8, 11 e 14 dias após a inoculação e calculada a área abaixo da curva de progresso de doença (AACPD. O controle preventivo foi mais eficiente no manejo da mancha bacteriana do tomateiro, e os diferentes biofertilizantes reduziram a severidade da doença.

  14. Vaginosis bacteriana por Gardnerella vaginalis: Nuevas enseñanzas desde la ecología molecular

    Directory of Open Access Journals (Sweden)

    Andrés Zúñiga

    2015-06-01

    Full Text Available La vaginosis bacteriana (VB, es la afección vaginal más frecuente en las mujeres en edad reproductiva generada por un desbalance en el ecosistema vaginal que ocasiona complicaciones severas para la salud reproductiva. Existen hipótesis de origen biológico que relacionan la presencia de organismos como Gardnerella vaginalis, Prevotella sp, Atopobium vaginae como la causa más frecuente relacionada con la vaginosis, los cuales logran desplazar poblacionalmente microorganismos con capacidad protectora del epitelio vaginal como Lactobacillus crispatus y Lactobacillus jensenii. En la actualidad y de acuerdo a la OMS, la vaginosis bacteriana estaría implicada en alteraciones durante el embarazo como parto pre termino, bajo peso al nacer, corioamnionitis, ruptura prematura de membranas (RPM, endometritis post parto, entre otras. En los últimos años, con base estudios apoyados en datos de patrones moleculares, así como tecnología de análisis de genomas, surge una visión mucho más completa de condiciones ecológicas y agentes participantes en la vaginosis bacteriana.

  15. Duchas vaginales y otros riesgos de vaginosis bacteriana

    Directory of Open Access Journals (Sweden)

    Natividad Chávez

    2009-07-01

    Full Text Available La vaginosis bacteriana (VB es una infección caracterizada por el cambio en la microflora de la vagina, se asocia con resultados adversos del embarazo y a la adquisición de infecciones de transmisión sexual (ITS, que incluyen el VIH. Objetivos. Identificar la asociación entre el uso de duchas vaginales y otros factores de riesgo con VB. Materiales y métodos. Se realizó un estudio transversal en 1252 mujeres que asistieron al servicio de planificación familiar de tres hospitales nacionales y un instituto especializado de Lima, durante el año 1997. Se utilizó un cuestionario estructurado donde se registró las variables sociodemográficas y las características del estilo de vida de las participantes. La VB fue diagnosticada mediante el puntaje de Nugent. Se empleó análisis de regresión logística para calcular odds ratio (OR e intervalos de confianza al 95%. Resultados. La edad promedio fue 25,1 ± 4,7 años, el 23,4% tenían más de 11 años de educación. La prevalencia de VB fue 20,1%. Las mujeres que practicaban duchas vaginales (OR: 2,28; IC95%: 1,0- 5,0, que tenían dos o más parejas sexuales (OR: 2,0; IC95%: 1,2-3,5 y que iniciaron una relación sexual a una edad temprana (OR: 1,4; IC95%: 1,0 -1,9 tuvieron mayor probabilidad de adquirir VB. Conclusiones. El uso de duchas vaginales es un factor de riesgo de VB en mujeres peruanas. Los programas destinados a la salud de la mujer deben abordar las repercusiones perjudiciales para la salud asociados con las duchas vaginales.

  16. DUCHAS VAGINALES Y OTROS RIESGOS DE VAGINOSIS BACTERIANA

    Science.gov (United States)

    Chávez, Natividad; Molina, Helfer; Sánchez, Jorge; Gelaye, Bizu; Sánchez, Sixto E.

    2010-01-01

    Vaginosis bacteriana (VB) es una infección caracterizada por el cambio en la microflora de la vagina, asociándose a resultados adversos del embarazo y a la adquisición de infecciones de transmisión sexual (ITS), incluyendo el VIH. En este estudio se buscó la asociación entre el uso de duchas vaginales y otros factores de riesgos con VB. Se usó un diseño observacional descriptivo transversal prospectivo, en 1,252 mujeres que asistieron al servicio de planificación familiar de tres hospitales nacionales (Dos de Mayo, Arzobispo Loayza, San Bartolomé) y el Instituto Materno Perinatal, durante el año 1997. Se utilizó un cuestionario estructurado donde se registraron variables socio demográficas y características del estilo de vida de las participantes. VB fue diagnosticada mediante el puntaje de Nugent. Se empleó análisis de regresión logística para calcular odds ratio (OR) e intervalos de confianza al 95%. La edad promedio de las participantes fue 25.1 ± 4,7 años, el 23.4% tenían más de 11 años de educación. La prevalencía de VB fue 20,1%. Las mujeres que practicaban duchas vaginales tuvieron 2.28 veces (OR = 2.28, IC 95% [1.0–5.0]) mayor probabilidad de tener VB comparado con aquellas que no lo practicaban. Tener dos o más parejas sexuales estuvo asociado con 2.0 veces (OR =2.0, IC 95% [1.2–3.5]) mayor probabilidad de adquirir VB comparado con aquellas que habían tenido solo una pareja sexual. Las participantes que iniciaron una relación sexual a una edad temprana tuvieron 1.4 veces (OR=1.4, IC 95% [1.0 –1.9]) mayor probabilidad de adquirir VB. El uso de duchas vaginales es un factor de riesgo de VB. Los programas destinados a la salud de la mujer deben abordar las repercusiones perjudiciales para la salud asociados con las duchas vaginales. PMID:21132048

  17. Advances in Boar Semen Cryopreservation

    Directory of Open Access Journals (Sweden)

    Heriberto Rodriguez-Martinez

    2011-01-01

    Full Text Available The present paper highlights aspects of the cryopreservation of boar semen, a species with particular large, fractionated ejaculates, and a cumbersome cryotechnology that had prevented its commercial application. With the dramatic increase of use of liquid pig semen for artificial breeding over the past decade, developments on cryopreservation alongside the routine use of stud boar semen for AI had been promoted. Recent advances in our laboratory, accommodating the best use of portions of the sperm-rich fraction of the ejaculate for cryopreservation of the sperm-peak portion (P1 and parallel use of the rest of the collected ejaculated spermatozoa, appears as a suitable commercial alternative.

  18. Bacteremia secundaria a una traslocación bacteriana, una complicación de la obstrucción intestinal mecánica

    OpenAIRE

    Chavarriaga Soto, Julián Ánd´res; Pontificia Universidad Javeriana, Bogotá, Colombia.

    2016-01-01

     Introduccion: la obstrucción intestinal es el trastorno quirúrgico más frecuente del intestino delgado. Como parte del manejo médico se ha sugerido el tratamiento empírico con antibióticos por el hallazgo patológico de traslocación bacteriana secundaria a la obstrucción. Métodos: se llevó a cabo una revisión de la literatura en las bases de datos Medline, Embase y PubMed y se incluyeron artículos con resultados sobre traslocación bacteriana, fisiología de la barrera intestinal, obstrucción i...

  19. Avian artificial insemination and semen preservation

    Science.gov (United States)

    Gee, G.F.; Risser, Arthur C.; Todd, Frank S.

    1983-01-01

    Summary: Artificial insemination is a practical propagation tool that has been successful with a variety of birds. Cooperative, massage, and electroejaculation and modifications of these three basic methods of semen collection are described for a variety of birds. Semen color and consistency and sperm number, moti!ity, and morphology, as discussed, are useful indicators of semen quality, but the most reliable test of semen quality is the production of fertile eggs. Successful cryogenic preservation of avian semen with DMSO or glycerol as the cryoprotectant has been possible. Although the methods for preservation require special equipment, use of frozen semen requires only simple insemination supplies

  20. Efecto de la pluviosidad y el brillo solar sobre la producción y características del semen en el pez Brycon henni (Pisces: Characidae)

    OpenAIRE

    Tabares, C. J.; Montoya, A. F.; Arboleda, L.; Echeverri, A; Restrepo, L. F.; M Olivera-Angel

    2014-01-01

    El pez Brycon henni es una especie endémica protegida por la legislación colombiana, que habita cuerpos de agua de zonas cafeteras (700-1900 m.s.n.m), comprendidas entre los 4º35’56’’ N y 74º04’51’’ W, con temperaturas que oscilan entre los 18 y los 28ºC. A pesar de las características promisorias de esta especie, su reproducción en cautiverio a nivel comercial no ha sido posible por falta de conocimientos básicos de su biología y comportamiento. El objetivo del presente trabajo fue caracteri...

  1. Partial replacement of chicken semen by turkey semen in artificial insemination of chickens.

    Science.gov (United States)

    Gavora, J S; Hodgson, G C

    1976-07-01

    Chicken semen undiluted, diluted with a diluent containing fructose and/or mixed with turkey semen was used to inseminate Leghorn hens. In two of three experiments there was an improvement in fertility from insemination by mixed semen as compared to semen diluted to the same extent with the diluent.

  2. Semenic Mountains’ alpine skiing area

    Directory of Open Access Journals (Sweden)

    Petru BANIAȘ

    2017-03-01

    Full Text Available The present paper presents, after a short history of alpine skiing which describes apparition, necessity, utility and universality of skiing during time, a comparative study referring to the alpine skiing domain in the Semenic Mountains area. In the paper are also presented general notions about alpine skiing methodology together with an ample description of the plateau area form Semenic Mountains, describing localization and touristic potential. Based on the SWOT analysis made for each slope, was realized a complex analysis of the entire skiing domain, an analysis which includes technical, financial, climatic and environmental aspects, along with an analysis of the marketing policy applied for the specific zone.

  3. Eficacia de cinco desinfectantes para la reducción bacteriana doméstica

    Directory of Open Access Journals (Sweden)

    Julián Stambullian

    2011-06-01

    Full Text Available El uso adecuado de hipoclorito de sodio, sales de amonio cuaternario y triclosán ha demostrado ser eficaz para eliminar gérmenes dentro del hogar. Nuestro objetivo fue evaluar la eficacia inmediata, a la semana y al mes del uso controlado de cinco productos con estos componentes, comparados con otros productos de uso habitual. Se incluyeron 32 hogares de clase media de la Ciudad de Buenos Aires y la periferia en un estudio con intervención, abierto, aleatorizado, y de grupos paralelos, durante 6 meses. La mitad de los hogares fue seleccionada para usar hipoclorito de sodio, sales de amonio cuaternario y triclosán en la cocina y el baño durante un mes. El grupo control mantuvo prácticas habituales de limpieza doméstica. Se tomaron muestras para recuento y tipificación bacteriana de los sitios estudiados: muestras basales (sin discriminación de grupo en cocina, que presentaron recuento bacteriano promedio de 66.0 UFC/cm²; baño: 40.1 UFC/cm². Las muestras inmediatas a la limpieza (sin discriminación de grupo: en cocina: 0.8 UFC/cm²; baño: <1 UFC/cm². A la semana (grupo intervenido vs. grupo control: cocina 18.0 vs. 32.5 UFC/cm²; baño 12.7 vs. 7.7 UFC/cm². Al mes (intervenido vs. control: cocina: 60.1 vs. 62.1 UFC/cm²; baño: 37.0 vs. 42.0 UFC/cm². Se observó una notable disminución de la carga bacteriana en ambos grupos, lo que sugiere que no sólo la calidad de los productos sino también la educación en el uso desempeñan un papel clave en la desinfección del hogar. Este enfoque podría ser una herramienta importante para prevenir infecciones transmitidas por alimentos, dado que los coliformes fecales predominaron ampliamente en todas las muestras tipificadas.

  4. Tiempo de latencia para semen colectado de Colossoma macropomum “Gamitana” en solución sacarosa

    Directory of Open Access Journals (Sweden)

    Ehrlich Llasaca-Calizaya

    2014-12-01

    Full Text Available El objetivo fue estimar el tiempo de latencia (almacenamiento, para el semen de Colossoma macropomum, “gamitana” en solución de 400 mM de Sacarosa. Se consideró aceptable los niveles de motilidad superiores al 40%, lo cual garantiza eficientes tasas de fertilización. Para el desarrollo del experimento se colectó 2 lotes de semen inmótiles de gamitana (inducidos con Conceptal®, los cuales posteriormente fueron activados con agua destilada. El primer lote estuvo constituido por semen en sacarosa 400 mM, puro, a temperatura ambiente y refrigerado (4°C. La motilidad fue evaluada, cada hora, hasta la 7ma hora post colecta. El segundo lote con un semen en sacarosa 400mM a temperatura refrigerada y evaluada cada 12 horas. Los resultados del primer lote de semen demuestran que a partir de la 7ma hora hacia delante los índices de motilidad caen significativamente por debajo del 40%. Los resultados del segundo lote demuestran la viabilidad de utilizar solución de sacarosa, como medio de conservación, para mantener semen refrigerado por 2 días y activarlos con agua destilada. El proceso de extraer y colocar repetidas veces la misma muestra en refrigeración, limita el tiempo de viabilidad de semen con sacarosa en 8 horas aproximadamente. La utilización de sacarosa como medio para almacenar semen inmotil viable de gamitana, ayuda a conservar los espermatozoides por tiempos relativamente cortos.

  5. Tiempo de latencia para semen colectado de Colossoma macropomum “Gamitana” en solución sacarosa

    Directory of Open Access Journals (Sweden)

    Ehrlich Llasaca-Calizaya

    2015-01-01

    Full Text Available El objetivo fue estimar el tiempo de latencia (almacenamiento, para el semen de Colossoma macropomum, “gamitana” en solución de 400 mM de Sacarosa. Se consideró aceptable los niveles de motilidad superiores al 40%, lo cual garantiza eficientes tasas de fertilización. Para el desarrollo del experimento se colectó 2 lotes de semen inmótiles de gamitana (inducidos con Conceptal®, los cuales posteriormente fueron activados con agua destilada. El primer lote estuvo constituido por semen en sacarosa 400 mM, puro, a temperatura ambiente y refrigerado (4°C. La motilidad fue evaluada, cada hora, hasta la 7ma hora post colecta. El segundo lote con un semen en sacarosa 400mM a temperatura refrigerada y evaluada cada 12 horas. Los resultados del primer lote de semen demuestran que a partir de la 7ma hora hacia delante los índices de motilidad caen significativamente por debajo del 40%. Los resultados del segundo lote demuestran la viabilidad de utilizar solución de sacarosa, como medio de conservación, para mantener semen refrigerado por 2 días y activarlos con agua destilada. El proceso de extraer y colocar repetidas veces la misma muestra en refrigeración, limita el tiempo de viabilidad de semen con sacarosa en 8 horas aproximadamente. La utilización de sacarosa como medio para almacenar semen inmotil viable de gamitana, ayuda a conservar los espermatozoides por tiempos relativamente cortos.

  6. Semen Anxiety: Materiality, Agency and the Internet.

    Science.gov (United States)

    Shand, Alex

    2007-12-01

    Semen is a potent cultural symbol of masculinity. The social life of semen is poorly understood because of the intensely personal nature of its being. But the Internet has opened up new avenues for people to explore sensitive issues without disclosing their identity. This paper examines a set of questions submitted anonymously for answering by a medical team over a three month period to a UK-based consumer health website. The questions are analysed for emergent themes and these are divided into three groups: those concerning the material quality of semen; semen relating to masturbation; and those that concern semen and potency. It argues that far from being a phenomenon isolated to non-western cultures, semen anxiety is present in the UK in the twenty-first century and is the expression of anxieties surrounding shifting gender roles and masculine identities.

  7. Semen Allergy Manifesting As Chronic Pruritus Vulva

    Directory of Open Access Journals (Sweden)

    Pavithran K

    1997-01-01

    Full Text Available A young woman of 24 with personal and family history of atopy development pruritus vulva each time after sexual intercourse with her husband. History of urticaria of sites of contact with semen on her thighs gave suspicion of contact urticaria. Positive wheal and flare response to pin prick test with semen, excellent therapeutic response to topical steroid and oral Cetirizine and non- recurrence of the problem after using condom by her husband confirmed the diagnosis of semen allergy.

  8. Semen parameters in polyzoospermic men

    Directory of Open Access Journals (Sweden)

    S. Sh. Khayat

    2015-01-01

    Full Text Available Polyzoospermia is extremely high (above 250 mln/ml sperm concentration with normal volume of ejaculate. In the laboratory of genetic disorders of reproduction of Research Centre for Medical Genetics in 2007–2012 years we analyzed more than 14 000 semen samples from infertile men and men with reproductive disorders in order to study the distribution and characteristics of spermatogenesis in polyzoospermic men. Only polyzoospermic men semen analysis results were interpretered in present article. Polyzoospermia (sperm count over 250 mln/ml in volume over 1,5 ml was detected in 191 semen samples (1.3 %. At the same time 15 % of the samples with polyzoospermia were normozoospermic. Among 85 % of the sperm pathology samples asthenozoospermia occured most frequently (77 %, astenoteratozoospermia was detected in 8 % of cases with polyzoospermia. The average proportion of vitality was 90.09 ± 10.02 %, normal morphology – 14.93 ± 8.51 %, and progressive motility – 17.72 ± 11.81 %. The average concentration of spermatozoa in the ejaculate in the examined samples was 313.29 ± 64.78 mln/ml. More than half of the tested samples with polyzoospermia had a concentration 250–300 mln/ml. Concentration of 450 mln/ml and more detected in 3 % of samples. The maximum total number of spermatozoa in the ejaculate in our study was more than 2 billion sperm cells and was observed in two polyzoospermic men. One of these patients had the maximum concentration (615 mln/ml also. We found a high correlation (r = 0.89; p < 0.01 between the volume of ejaculate and the total number of spermatozoa. Immature germ cells from ejaculate were estimated in 7 patients. There was a partial spermatogenesis arrest at meiosis I prophase in 5 out 7 of the examined semen samples. 

  9. Parámetros protéicos del plasma seminal y su relación con la calidad del semen en toros de la raza nelore (bos taurus indicus

    Directory of Open Access Journals (Sweden)

    Ana Sánchez

    2006-10-01

    Full Text Available El propósito de este estudio fue investigar la presenciae incidencia de bandas proteicas específicas delplasma seminal en toros Nelore, completa y parcialmente aptos para la actividad reproductiva. Se utilizaron 68 ejemplares; 20 de variedad Padrón y 48 Mochos, con edad media de 4 años. En el perímetro escrotal (35,05±0,49 cm e 33,30±0,39cm, índice de masa corpórea (302,62±5,87 e 284,19±5,15Kg|m2 hubo diferencia (p<0,05 entre las variedades Padrón y Mocho, respectivamente. Con respecto al peso corpóreo (627,70±11,37 e 611,58±8,66Kg; la altura (1,44±0,01e 1,47±0,01m; el volumen del eyaculado (5,82±0,48 e 5,17±0,29 mL, la motilidad espermática progresiva (73,50±2,81% e75,62±0,97%, el vigor espermático (4,30±0,19 e4,27±0,11 y motilidad en masa (4,27±0,11 e3,33±0,23 no se presentó diferencia (p>0,05. En morfología espermática, tampoco hubo desigualdad entre las variedades Padrón y Mocho, respectivamente con 5,06 ± 8,20% e 5,32 ± 6,40% de defectos mayores; 9,91±6,74% e 8,36±6,06% para los defectos menores; e 14,76±13,20% e 13,82±12,61% para los defectos totales. La electroforesis del plasma seminal reveló bandas proteicas con pesos entre 5 a 105 KDa. En el 100% de toros aptos para la reproducción, la proteína con pesos de 13 Kda estuvo presente. De la misma forma ocurrió con las bandas de 20 KDa. El resto de las bandas proteicas mostraron presencia con diferentes porcentajes de incidencia en toros aptos o parcialmente aptos para la actividad reproductiva. Las dos variedades estudiadas hicieron evidente la adaptación reproductiva eficaz en condiciones de clima semejantes.

  10. Stainless steel welding and semen quality

    DEFF Research Database (Denmark)

    Jelnes, J E; Knudsen, Lisbeth E.

    1988-01-01

    Questionnaire studies of patients from fertility clinics suggest that welders may have an increased risk of reduced semen quality. In this study, welders and nonwelders from the same plants were asked to provide blood, urine, and semen samples. Urine was analyzed for chromium and nickel, and for ...

  11. Ebola Virus Persistence in Semen Ex Vivo.

    Science.gov (United States)

    Fischer, Robert J; Judson, Seth; Miazgowicz, Kerri; Bushmaker, Trent; Munster, Vincent J

    2016-02-01

    On March 20, 2015, a case of Ebola virus disease was identified in Liberia that most likely was transmitted through sexual contact. We assessed the efficiency of detecting Ebola virus in semen samples by molecular diagnostics and the stability of Ebola virus in ex vivo semen under simulated tropical conditions.

  12. Evaluación de la eficacia de dos prescripciones de cepillos dentales en la remoción de placa bacteriana en pacientes ortodóncicos

    OpenAIRE

    Zúñiga García,D; Pastén Castro,EJ; Araya-Díaz,PA; Palomino Montenegro,H

    2012-01-01

    La aparatología empleada en los tratamientos de ortodoncia, favorece la retención de placa bacteriana y dificulta su eliminación por parte del paciente, aumentando el riesgo de desarrollar caries, manchas blancas y enfermedad periodontal. Diferentes cepillos dentales se han desarrollado para facilitar la mantención de una adecuada higiene oral en estos pacientes, sin embargo, se ha observado que los cepillos comunmente indicados (cepillo ortodóncico mas cepillo unipenacho), no siempre son bie...

  13. Caracterización de pacientes con bronconeumonía bacteriana asociada a la ventilación artificial mecánica

    OpenAIRE

    Danilo Eranio Utra Cardoso; Leonila Atencio González; Aida Esther Leyva Peña; Gladis Marrero Ávila; Idalia Arcias Castillo

    2015-01-01

    Fundamento: las neumonías nosocomiales constituyen hoy en día un problema de salud a nivel mundial. En las unidades de cuidados intensivos de las instituciones hospitalarias ingresa un elevado número de pacientes que requieren ventilación artificial mecánica, factor de riesgo asociado a este tipo de infecciones. Objetivo: caracterizar a los pacientes con neumonías bacterianas asociadas a la ventilación artificial mecánica, ingresados en la sala de cuidados intensivos del Hospital General D...

  14. Cerclaje profiláctico en mujeres con nacimientos prematuros espontáneos previos, asociados con infección bacteriana ascendente

    OpenAIRE

    Ovalle S,Alfredo; Valderrama C,Oscar; Rencoret P,Gustavo; Fuentes G,Ariel; del Río V,María José; Kakarieka W,Elena; Martínez T,María Angélica; Pizarro S,Dagoberto

    2012-01-01

    Objetivo: Determinar la eficacia del cerclaje cervical profiláctico en pacientes con embarazos únicos, cérvix >25 mm e historia de nacimientos prematuros espontáneos, asociados con infección bacteriana ascendente (IBA). Métodos: Estudio clínico en pacientes con embarazos únicos y partos prematuros y/o abortos de 2° trimestre espontáneos previos, sin partos de término, asociados con IBA. Se incluyeron los casos con longitud cervical de >25 mm al ingreso. Se comparó el cerclaje cervical h...

  15. Medición de cambios cuantitativos de la microbiota subgingival posterior a la remoción de placa bacteriana supragingival

    OpenAIRE

    C. Godoy; Melej,C; Silva, N

    2010-01-01

    Uno de los campos de interés en el estudio de la microbiología periodontal para muchos investigadores ha sido identificar si es posible que los microorganismos responsables del origen y progresión de la enfermedad periodontal que habitan sobre el margen gingival (supragingival) y bajo este (subgingival) tengan una relación directa que permita mantener interacciones influyentes en el crecimiento y desarrollo de las diferentes especies bacterianas que habitan en los tejidos periodontales. Por l...

  16. Aislamiento e identificación de diez cepas bacterianas desnitrificantes a partir de un suelo agrícola contaminado con abonos nitrogenados proveniente de una finca productora de cebolla en la Laguna de Tota, Boyacá, Colombia

    OpenAIRE

    ostos ortiz, olga lucia; Benavides López de Mesa, Joaquín; Quintero, Gladys

    2006-01-01

    Este articulo presenta los resultados de la versatilidad adaptativa y tolerancia de una consorcio bacteriano constituido por 10 cepas bacterianas endémicas de un suelo agrícola contaminado, proveniente de una finca productora de cebolla ubicada en la laguna de Tota en Aquitania, Boyacá, Colombia, expuesto al uso incontrolado de fertilizantes nitrogenados. La consorcio bacteriano aislado del suelo está conformado por: Bacillus megaterium, Bacillus licheniformis, Pseudomonas stutzeri, Acinetoba...

  17. Actividad sialidasa en mujeres con vaginosis bacteriana Sialidase activity in women with bacterial vaginosis

    Directory of Open Access Journals (Sweden)

    Adriana M. Ombrella

    2006-04-01

    Full Text Available La vaginosis bacteriana (VB es un síndrome caracterizado por el sobrecrecimiento bacteriano de flora endógena Gram negativa, que desplaza a la flora lactobacilar normal. Dentro de las enzimas bacterianas, las sialidasas han sido consideradas factores de virulencia de muchos microorganismos patógenos que colonizan las distintas mucosas. Su presencia en fluidos vaginales puede estar correlacionada con VB. El propósito de este estudio fue comprobar la actividad de dicha enzima en mujeres con este síndrome y sin evidencia clínica de infección genital. Se estudiaron 112 mujeres (51 fueron pacientes con VB y 61 mujeres con flora colonizante habitual. Para la cuantificación de la actividad sialidasa se empleó la técnica basada en la hidrólisis enzimática de un derivado ácido del ácido metoxifenil acetil murámico. En la población estudiada se encontró que ambos grupos mostraron valores comprendidos entre 0.5 a 5.1 nmoles de metoxifenol, mientras que 11 de 52 pacientes con VB (21.17%, registraron valores superiores a 5.1 nmoles. La presencia de actividad sialidasa solamente no es índice de VB, excepto para valores mayores de 5.5 nmoles de metoxifenol, producidos en la reacción enzimática.Bacterial vaginosis (VB is a syndrome characterized by overgrowth of endogenous Gram negative bacterial flora and the lack of the normal flora. Within bacterial enzymes, sialidases have been considered a virulence factor of many pathogenic microorganisms colonizing the different mucous membranes. Their presence in vaginal discharges can be correlated with VB. The aim of this study was to detect the activity of this enzyme in women with this syndrome and without clinical evidence of genital infection. Out of a total 112 women studied, 51 were patients with VB and the other 61 women presented normal vaginal flora. For the quantification of enzyme activity, the technique based on the enzymatic hydrolysis of a derivative acid of the acetyl metoxifenil

  18. Antithrombotic lipids from Semen Persicae.

    Science.gov (United States)

    Yang, Nian-Yun; Liu, Li; Tao, Wei-Wei; Duan, Jin-Ao; Liu, Xun-Hong; Huang, Su-Ping

    2011-10-01

    Chemical investigation of Semen Persicae has led to the isolation of decane (1), triolein (2), nonacosanoic acid (3), oleic acid ethyl ester (4), palmitic acid (5), oleic acid (6) and 15,16-dihydroxy-9Z,12Z-octadecadienoic acid 2,3-dihydroxypropyl ester (7). Amongst these, compound 7 is a new lipid. Their structures were elucidated by chemical and extensive spectral analysis. Their anticoagulative activities were also evaluated in vitro, which showed that petroleum ether extract and compounds 5-6 could significantly prolong thrombin time while methanol extract could obviously inhibit platelet aggregation.

  19. Recomendaciones para el manejo de la faringoamigdalitis aguda del adulto

    OpenAIRE

    Cots, Josep M; Juan-Ignacio Alós; Mario Bárcena; Xavier Boleda; José L. Cañada; Niceto Gómez; Ana Mendoza; Isabel Vilaseca; Carles Llor

    2015-01-01

    La faringoamigdalitis aguda (FAA) en el adulto es una de las enfermedades infecciosas más comunes en la consulta del médico de familia. La etiología más frecuente es viral. Dentro de la etiología bacteriana, el principal agente responsable es Streptococcus pyogenes o estreptococo β-hemolítico del grupo A (EBHGA), causante del 5-30% de los casos. En el manejo diagnóstico, las escalas de valoración clínica para predecir la posible etiología bacteriana, son una buena ayuda para seleccionar a qué...

  20. Meningoencefalitis Bacteriana. Estudio clínico-epidemiológico. Hospital Pediátrico Provincial de Holguín. 2008-2009.

    Directory of Open Access Journals (Sweden)

    Michel Escalona Martín

    2010-01-01

    Full Text Available Presentó un estudio clínico-epidemiológico de la Meningoencefalitis Bacteriana en el Hospital Pediátrico de Holguín en el período de enero del 2008 a diciembre del 2009. Al ingreso fueron diagnosticados 13 pacientes, al egreso el diagnóstico definitivo fue de 11 pacientes. El grupo de edad más afectado resultó ser hasta los cuatro años y se reportó un mayor número decasos en el primer semestre para ambos años. Las manifestaciones clínicas frecuentes resultaron ser la fiebre, rigidez de nuca, irritabilidad y alteraciones de conciencia. Se cuantificó un mayor número de pacientes con celularidad superior a 1000 x106/L. En más del 50% de los pacientes fueron observadas formas bacterianas en el Gram y el Streptococcus pneumoniae fue el germen predominante. En el año 2008 el 20% de los pacientes falleció y el 40% presentó secuelas, en el 2009 el 33.4% de los pacientes fallecieron y no se reportaron secuelas.

  1. Estudio de la interacción de bacterias implicadas en la formulación de placa dentro-bacteriana con superficies de titanio comercialmente puro in vitro y su asociación con la peri-implantitis.

    OpenAIRE

    Rodríguez Hernández, Ana Guadalupe

    2009-01-01

    La peri-implantitis puede poner en riesgo la integridad funcional de un implante dental, ya que dependiendo de la severidad del daño, éste puede ocasionar la pérdida irremediable del implante. Dicho padecimiento está asociado directamente con la formación de la placa dento-bacteriana sobre la superficie del implante.En este trabajo de tesis se estudiarón, por un lado las propiedades de superficie de 6 tipos diferentes de tratamientos utilizados en implantes dentales (tales como: la rugosidad,...

  2. Métodos de colección de semen en camélidos sudamericanos

    Directory of Open Access Journals (Sweden)

    Pacheco Curie, Joel Iván

    2008-05-01

    Full Text Available La colección de semen depende de una buena y constante producciónespermática para que la calidad del semen sea buena. Las técnicas decolección de semen están bastante desarrolladas en otros animales,especialmente en rumiantes domésticos en los cuales ya es unprocedimiento de rutina, pero en camélidos, dadas las especialescaracterísticas reproductivas, anatómicas y fisiológicas de estas especies, esta colección es bastante dificultosa y no existe un protocolo recomendado y una técnica optima, así como su manejo posterior.

  3. Study on Semen Freezing Preservation of German Shepherd Dogs

    Institute of Scientific and Technical Information of China (English)

    Huang He; Tian Ya-guang; Zheng Peng; Zhang Gui-xue

    2012-01-01

    In order to prolong semen preservation and improve pregnancy rate, semen freezing was studied with German shepherd dogs for experimental animals. The semen of four dogs was collected 40 times in four dilution frozen into two formulations, according to the sperm motility to compare the advantages and disadvantages. The result indicated that the sperm motility of the pellet frozen semen in dilute 2 was significantly higher than that in dilution 1, 3, and 4 (P0.01). The sperm motility of dogs semen with fried smoked method was notablely higher than that of frozen semen of program method (P0.01). The dilution which contained yolk-Tris mainly was the best; the pellet semen frozen with fried smoked method was superior to tuble semen frozen with program freezing method; sperm motility of pellet semen was higher than that of tuble semen in the same dilution. The conception rate and litter size of the natural matting were higher than AI.

  4. Semen characteristics in pubertal boys. I. Semen quality after first ejaculation.

    Science.gov (United States)

    Janczewski, Z; Bablok, L

    1985-01-01

    Semen specimens from 134 pubertal boys were examined, and some 274 assays were made. An analysis of the biological quality of semen in relation to the period of time after first ejaculation brings high values of statistical dependence of the volume of semen, its liquefaction, spermatozoal concentration, percentage of morphologically normal forms of spermatozoa, and normal spermatozoal motility on the period of time after first ejaculation. Normal figures for semen volume, semen liquefaction, spermatozoal concentration, and morphology are observed 12-14 months after first ejaculation. The percentage of normally motile spermatozoa becomes standard 21-23 months after first ejaculation. There were changes in semen characteristics from azoospermia through cryptozoospermia, oligozoospermia, and asthenozoospermia to normospermia. Azoospermia dominates until the fifth month after the first ejaculation, oligozoospermia from the sixth to the eleventh month, asthenozoospermia from the twelfth to the twentieth month, and normospermia from the twenty-first month.

  5. Semen preparation techniques for intrauterine insemination

    NARCIS (Netherlands)

    Boomsma, C. M.; Heineman, M. J.; Cohlen, B. J.; Farquhar, C.

    2007-01-01

    Background Semen preparation techniques for assisted reproduction, including intrauterine insemination (IUI), were developed to separate the motile morphological normal spermatozoa. Leucocytes, bacteria and dead spermatozoa produce oxygen radicals that negatively influence the ability to fertilize t

  6. Artificial insemination of cranes with frozen semen

    Science.gov (United States)

    Gee, G.F.; Sexton, T.J.; Lewis, J.C.

    1979-01-01

    For the first time (1978) artificial insemination (AI) with frozen greater sandhill crane (Grus canadensis tabida) semen resulted in fertile eggs and chicks. During the 2 year (1977-78) study, 6 of 27 eggs produced were fertile. Three chicks hatched. Semen samples used for insemination were frozen and stored in liquid nitrogen for two months or less. Recent improvements in the laboratory indicated that a more effective sample can be prepared and greater fertility rates should be expected.

  7. Semen collection using phantom in dromedary camel.

    Science.gov (United States)

    Ziapour, S; Niasari-Naslaji, A; Mirtavousi, M; Keshavarz, M; Kalantari, A; Adel, H

    2014-12-10

    Semen collection is relatively long, unsafe, and tedious procedure in dromedary camel. The innovation of safe, hygienic, and simple approach to collect semen could make great progress in development of AI program in this species. This study investigated two methods of semen collection using phantom and artificial vagina in dromedary camel. Semen was collected using phantom (n = 4 bulls; 26 collections) and artificial vagina (n = 6 bulls; 11 collections) and diluted with INRA96 at the ratio of 1:10. The duration of semen collection, semen parameters, and morphometric features of sperm were evaluated. For specimen collected through phantom and AV, the respected duration of semen collection (411.2 ± 48.19 vs 326 ± 37.05 sec), volume (6.6 ± 0.87 vs 6 ± 1.57 ml), osmolarity (328 ± 1.6 vs 319.4 ± 3.21 mOsm/kg H2O), pH (7.7 ± 0.06 vs 7.9 ± 0.16) of semen, concentration (161.4 ± 44.05 × 10(6)/mL vs 160.2 ± 58.42 × 10(6)/mL), total motility (84.1 ± 1.89 vs 78.3 ± 3.97%), progressive forward motility (45.5 ± 3.69 vs 44.3 ± 6.41%), live percentage (72.2 ± 3.11 vs 76 ± 2.53%), and plasma membrane integrity (61.5 ± 2.49 vs 58.9 ± 4.19%) of sperm were similar (P > 0.05). The number of specimens contaminated with visible particles was greater using AV (72.7%) compared to phantom (0%; P dromedary camel.

  8. Applications of sexed semen in cattle production.

    Science.gov (United States)

    Hohenboken, W D

    1999-12-01

    Sexed semen will contribute to increased profitability of dairy and beef cattle production in a variety of ways. It could be used to produce offspring of the desired sex from a particular mating to take advantage of differences in value of males and females for specific marketing purposes. Commercial dairy farmers, those who produce and market milk, could use sexed semen to produce replacement daughters from genetically superior cows and beef crossbred sons from the remainder of their cow population. To increase the rate of response to selection, seedstock dairy cattle breeders could produce bulls for progeny testing from a smaller number of elite dams by using sexed semen to ensure that all of them produced a son. Using sexed semen could then reduce the cost of progeny testing those bulls, because fewer matings would be necessary to produce any required number of daughters. Commercial beef cattle farmers, producing animals for eventual slaughter, could use sexed semen to capitalize on the higher value of male than female offspring for meat production. They could also use sexed semen to produce specialized, genetically superior replacement heifers from as small a proportion of the herd as possible. This would allow the remainder of the herd to produce male calves from bulls or breeds with superior genetic merit for growth, feed conversion efficiency, and carcass merit. Single-sex, bred-heifer systems, in which each female is sold for slaughter soon after weaning her replacement daughter, would be possible with the use of X-chromosome-sorted semen. Use of sexed semen would make terminal crossbreeding systems more efficient and sustainable in beef cattle. Fewer females would be required to produce specialized maternal crossbred daughters, and more could be devoted to producing highly efficient, terminal crossbred sons.

  9. [Morphological semen changes in Chlamydia trachomatis infection].

    Science.gov (United States)

    Maciejewski, Z; Dziecielski, H; Swierczyński, W; Semmler, G

    1989-06-01

    Semen was examined in 150 men patients of the Andrology Clinic for demonstration of Chlamydia trachomatis and for analysis of the effect of this infection on semen quality depression. A correlation was noted between the degree of infection (large number of organisms per field of vision) and such changes as cryptozoospermia, azoospermia, asthenozoospermia, teratozoospermia, oligoasthenozoospermia, asthenoteratozoospermia. Of interest was a high proportion of infection (56%) with Ch. trachomatis in this group.

  10. Detection of Campylobacter or Salmonella in turkey semen and the ability of poultry semen extenders to reduce their concentrations.

    Science.gov (United States)

    Donoghue, A M; Blore, P J; Cole, K; Loskutoff, N M; Donoghue, D J

    2004-10-01

    Campylobacter and Salmonella are the most commonly reported pathogens causing foodborne illness in the United States. In turkeys, the potential that semen used for artificial insemination is contaminated with these foodborne pathogens has not been investigated. Because semen on turkey farms is pooled and then used to inseminate multiple hens, contaminated semen could easily spread these bacteria throughout entire flocks via artificial insemination. The objectives of this study were to 1) determine if semen from commercial turkey farms contained these foodborne pathogens and 2) if present, evaluate the efficacy of semen extenders to reduce or eliminate Campylobacter and Salmonella from semen. Semen was collected from randomized pools of ejaculates from 10 to 30 toms per farm from 6 flocks over a 7-wk period and, on occasion, was found to contain Campylobacter, Salmonella, or both. To evaluate the efficacy of semen extenders to reduce or eliminate pathogens, pooled ejaculates were challenged with Campylobacter or Salmonella and treated with commercial poultry extenders containing various concentrations of antibiotics or an antibiotic combination previously demonstrated to remove Campylobacter from mammalian semen. Results demonstrate that commercial turkey semen may contain Campylobacter or Salmonella, and the semen extenders tested either did not reduce the bacteria or reduced but did not eliminate these bacteria from semen. We concluded that semen may be a potential vehicle for Campylobacter transfer to hens, and, if this is true, development of a method for eliminating pathogens in semen before insemination could reduce the risk of colonization.

  11. Una nueva ultraestructura de ópalo CT en silcretas. Posible indicador de influencia bacteriana

    Directory of Open Access Journals (Sweden)

    Bustillo, Mª A.

    1995-04-01

    Full Text Available Electron microscope observations of opaline silcretes found in the playa of a Miocene shallow lake reveal new opal CT microstructures. These microstructures are made up of fibres, filaments or rods, which are formed, in tum by coalesced microspheres or microhemispheres (0,1-0,2 µ diameter. They are up to 10 µ in length although larger ones could exist. They are straight, bent, wavy or even hairpin shaped. These microstructures comprise part of the opaline mass of the silcretes and are very similar to both neogenic opaline structures which are formed by bacterial intluence and to silicified biofilms. Therefore microbial mediation could exist in the genesis of this opal CT, although a possible inorganic origin by silicification of sepiolite is also considered.Un estudio mediante MEB de silcretas miocenas opalinas formadas por silicificación de playas en un ambiente de «playa-lake» pone de manifiesto la existencia de una nueva microestructura de ópalo CT. Esta microestructura denominada en general fibrosa, se manifiesta por la presencia de multitud de fibras, filamentos o varillas formadas por la alineación de microesferas o microhemisferas que presentan un diámetro entre 0,1 y 0,2 µ. Las fibras, filamentos o varillas tienen formas variables: rectas, curvadas, onduladas y «en horquilla», y presentan longitudes de hasta 10 µ, aunque podrían existir longitudes mayores. La disposición organizada de las microesferas en filamentos o fibras, constituyendo la masa general del ópalo, y su parecido con microestructuras opalinas que han sido formadas por influencia bacteriana, hacen considerar esta hipótesis, aunque no se puede descartar totalmente un origen inorgánico por silicificación de sepiolita.

  12. Acumulación de antibioticos y su efecto sobre la comunidad bacteriana presente en sedimentos de piscinas camaroneras

    OpenAIRE

    Montoya, Nelson; Uyaguari, Miguel; Sotomayor, Mariuxi

    2003-01-01

    ACUMULACION DE ANTIBIOTICOS Y SU EFECTO SOBRE LA COMUNIDAD BACTERIANA PRESENTE EN SEDIMENTOS DE PISCINAS CAMARONERAS La mayor preocupación que ha surgido al considerar el uso de agentes antibacteriales en acuicultura, es la posibilidad de que sus residuos puedan estimular la presencia de resistencia bacteriana.

  13. CIRCULACION DEL NEUMOCOCO RELACIONADO A CAMBIOS EN LOS FACTORES CLIMÁTICOS EN LA PROVINCIA DE LA RIOJA

    OpenAIRE

    Patricia Cordoba, PhD; Mónica Romanazzi; Sonia Flores; Patricia Torres; Jose Maria Barrios

    2003-01-01

    Resumen: Streptococo pneumoniae (Neumococo), Neisseria meningitidis y Haemophilus influenzae producen meningitis bacterianas. Este trabajo describe las caracteristicas del aumento de casos de meningitis bacterianas en la Provincia de la Rioja, Argentina. Se realizó un estudio descriptivo retrospectivo de las meningitis desde 1997 hasta 2001. Se estudió la circulación de los agentes etiologicos; la distribución de las neumonías y bacteriemias producidas por neumococo; las variaciones de la ...

  14. Síntesis intratecal de C3c e inmunoglobulinas en niños con meningoencefalitis bacteriana

    Directory of Open Access Journals (Sweden)

    Bárbara Padilla-Docal

    2008-12-01

    Full Text Available Las meningoencefalitis bacterianas constituyen una fuente importante de morbilidad, mortalidad y discapacidad en diferentes regiones del mundo. El objetivo del presente trabajo es conocer si el sistema de complemento puede estar involucrado en la lisis de las bacterias productoras de meningoencefalitis a través de la liberación de C3c al líquido cefalorraquídeo. Se estudiaron siete pacientes con edad promedio de 3 años, que ingresaron en el Hospital Pediátrico de San Miguel del Padrón, a los que se les realizó una punción lumbar diagnóstica y se les aislaron los gérmenes siguientes: Neisseria meningitidis, Streptococcus pneumoniae y Haemophilus influenzae. La cuantificación de los niveles de C3c, albúmina e inmunoglobulinas mayores en suero y líquido cefalorraquídeo se realizó en placas de inmunodifusión radial. Los resultados obtenidos fueron recogidos en un reibergrama. El total de los pacientes estudiados mostraron síntesis intratecal del componente C3c del sistema de complemento. Este hecho evidenció la activación de este sistema en alguna de sus vías y que una vez cumplidas sus funciones biológicas, ha sufrido un proceso de degradación y liberación al LCR en forma de C3c.

  15. Scientists Map DNA of Zika Virus from Semen

    Science.gov (United States)

    ... news/fullstory_161474.html Scientists Map DNA of Zika Virus From Semen It's another step in trying to ... complete genetic "blueprint" -- genome -- of a sample of Zika virus derived from semen has been obtained by researchers. ...

  16. Ebola May Be Present in Semen for Year or More

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_160697.html Ebola May Be Present in Semen for Year or ... 31, 2016 TUESDAY, Aug. 30, 2016 (HealthDay News) -- Ebola virus stays present in semen longer than previously ...

  17. History of febrile illness and variation in semen quality

    DEFF Research Database (Denmark)

    Carlsen, Elisabeth; Andersson, Anna-Maria; Petersen, Jørgen Holm

    2003-01-01

    The purpose of this study was to analyse the effect of a history of febrile illness on semen quality.......The purpose of this study was to analyse the effect of a history of febrile illness on semen quality....

  18. Demonstration of DSI-semen--A novel DNA methylation-based forensic semen identification assay.

    Science.gov (United States)

    Wasserstrom, Adam; Frumkin, Dan; Davidson, Ariane; Shpitzen, Moshe; Herman, Yael; Gafny, Ron

    2013-01-01

    Determining whether the source tissue of biological material is semen is important in confirming sexual assaults, which account for a considerable percentage of crime cases. The gold standard for confirming the presence of semen is microscopic identification of sperm cells, however, this method is labor intensive and operator-dependent. Protein-based immunologic assays, such as PSA, are highly sensitive and relatively fast, but suffer from low specificity in some situations. In addition, proteins are less stable than DNA under most environmental insults. Recently, forensic tissue identification advanced with the development of several approaches based on mRNA and miRNA for identification of various body fluids. Herein is described DNA source identifier (DSI)-semen, a DNA-based assay that determines whether the source tissue of a sample is semen based on detection of semen-specific methylation patterns in five genomic loci. The assay is comprised of a simple single tube biochemical procedure, similar to DNA profiling, followed by automatic software analysis, yielding the identification (semen/non-semen) accompanied by a statistical confidence level. Three additional internal control loci are used to ascertain the reliability of the results. The assay, which aims to replace microscopic examination, can easily be integrated by forensic laboratories and is automatable. The kit was tested on 135 samples of semen, saliva, venous blood, menstrual blood, urine, and vaginal swabs and the identification of semen vs. non-semen was correct in all cases. In order to test the assay's applicability in "real-life" situations, 33 actual casework samples from the forensic biological lab of the Israeli police were analyzed, and the results were compared with microscopic examination performed by Israeli police personnel. There was complete concordance between both analyses except for one sample, in which the assay identified semen whereas no sperm was seen in the microscope. This

  19. Semen abnormalities with SSRI antidepressants.

    Science.gov (United States)

    2015-01-01

    Despite decades of widespread use, the adverse effect profile of "selective" serotonin reuptake inhibitor (SSRI) antidepressants has still not been fully elucidated. Studies in male animals have shown delayed sexual development and reduced fertility. Three prospective cohort studies conducted in over one hundred patients exposed to an SSRI for periods ranging from 5 weeks to 24 months found altered semen param-eters after as little as 3 months of exposure: reduced sperm concentration, reduced sperm motility, a higher percentage of abnormal spermatozoa, and increased levels of sperm DNA fragmentation. One clinical trial showed growth retardation in children considered depressed who were exposed to SSRls. SSRls may have endocrine disrupting properties. Dapoxetine is a short-acting serotonin reuptake inhibitor that is chemically related to fluoxetine and marketed in the European Union for men complaining of premature ejaculation. But the corresponding European summary of product characteristics does not mention any effects on fertility. In practice, based on the data available as of mid-2014, the effects of SSRI exposure on male fertility are unclear. However, it is a risk that should be taken into account and pointed out to male patients who would like to father a child or who are experiencing fertility problems.

  20. SIFAT FISIKA PAPAN SEMEN PARTIKEL PELEPAH RUMBIA

    Directory of Open Access Journals (Sweden)

    Gusti Ahmad Rahmat Thamrin

    2016-09-01

    Full Text Available ABSTRACT. Manufacture of cement particle board and testing physical properties are implemented in the laboratory of panels of wood forest products Technology majors Lambung Mangkurat University. Research on physical properties include water content, density, water absorption, and the development of thick. The experimental use is a random complete ral ( 2 nonfaktorial by remedial treatment and 5 times , a particle that is making cement with raw materials pelepah rumbia without the skin and cement making a particle with raw materials pelepah rumbia with skin . Standard used for comparison is SNI-03-2104-1991 . The average levels of the water between the semen of a particle midrib metroxylon sagu without the skin and board cement particles midrib metroxylon sagu with skin successive adalah13,02 % and 10,75 %. Both treatment entry in the SNI (maximum 14%. The average value of the density of the particle cement board without bark and branches and kiray cement particle board with the skin in a row as kiray is 0.73 gr/cm3 and 0.90 gr/cm3. Both treatment entry in the SNI (minimum 0.57 per gr/cm3.The average value of water absorption of cement particle board as kiray without skin and cement particle board with the skin in a row as kiray is 43,86% and 35.86%. The second treatment was not entered in the SNI (10-30%. Keywords: cement particle board, Rumbia’s Midrib ABSTRAK.    Pembuatan papan semen partikel dan pengujian sifat fisika dilaksanakan di Laboratorium Panel-Panel Kayu jurusan Teknologi Hasil Hutan Universitas Lambung Mangkurat. Penelitian sifat fisika meliputi kadar air, kerapatan, penyerapan air, dan pengembangan tebal. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL nonfaktorial dengan 2 perlakuan dan 5 kali ulangan, yaitu pembuatan papan semen partikel dengan bahan baku pelepah rumbia tanpa kulit dan pembuatan papan semen partikel dengan bahan baku pelepah rumbia dengan kulit. Standar yang digunakan untuk perbandingan

  1. Contaminación bacteriana en concentrados de plaquetas de caballos Bacterial contamination in platelet concentrates of horses

    Directory of Open Access Journals (Sweden)

    ME Álvarez

    2010-01-01

    Full Text Available Los objetivos del estudio fueron: 1 evaluar el riesgo de contaminación bacteriana de concentrados autólogos de plaquetas (APCs de caballos obtenidos mediante el método del tubo en tres condiciones técnicas diferentes (cámara de flujo laminar y en ambiente de laboratorio limpio con mechero o sin mechero, 2 identificar los puntos críticos del proceso de preparación de los APCs con posibilidad de contaminación bacteriana y 3 identificar las bacterias potencialmente contaminantes en el proceso. Se tomaron muestras bacteriológicas de la piel (rasurada o no del sitio de venopunción de 15 caballos, antes y después de ser desinfectados; manos y garganta del operario; tapones de los tubos donde se procesó la sangre; medio ambiente donde se tomaron las muestras de sangre, ambiente de laboratorio, cámara de flujo laminar, estufa bacteriológica y de los APCs obtenidos bajo condiciones técnicas. Se aislaron bacterias de la piel equina sin desinfectar, manos y garganta del operario y del lugar donde se tomaron las muestras de sangre. No se aislaron bacterias de los tapones de los tubos, medio ambiente del laboratorio, cámara de flujo laminar, ni de los APCs. Las bacterias aisladas fueron biota normal de la piel equina, de la piel y garganta humana, y contaminantes medioambientales. En conclusión, los APCs pueden ser obtenidos mediante el método del tubo en un ambiente limpio.The aims of the study were to: 1 assess the risk of bacterial contamination in equine platelet concentrates (PCs obtained by the tube method under three technical conditions (laminar flow cabinet or in a clean laboratory environment both with burner and without burner 2 identify the critical points of the process of PCs preparation with risk of bacterial contamination; and 3 identify the potential bacterial contaminants in the process. Bacteriological samples were taken from the skin (shaved or unshaved of the venipuncture site in 15 horses, both before and after being

  2. Relationship between Thyroid Profile and Semen Quality

    Directory of Open Access Journals (Sweden)

    Manoj kumar Sharma, Deepak Parchwani, Pankaj Maheria, Amit Upadhyah

    2012-01-01

    Full Text Available Background: Endogenous hormones are critical to spermatogenesis and maintenance of male reproductive function. Follicle stimulating hormone (FSH, luteinizing hormone (LH, inhibin B and testosterone all serve important and well-known functions in the male hypothalamopituitary-gonadal axis and male reproduction. However, the potential relationship between other hormones, including thyroid hormones, and semen quality are still not completely understood. Thus in the present study an attempt has been made to report the degree of associations between thyroid hormones and semen quality. Methods: Fifty-five men were recruited from an infertility clinic between August 2010 to May 2011. Fresh semen samples were assessed for quality (concentration, motility and morphology and the serum levels of Tetraiodothyronine (T4, Triiodothyronine (T3, and Thyroid stimulation hormone (TSH were measured. Result & Conclusion: We have found that though men with abnormal semen profile had higher total T3, T4 concentrations and lower TSH concentrations compared to those with normal semen profile, only T4 showed significant increase and further it was found that only total T4 was significantly associated with asthenozoospermia. Further studies and observation are needed on a larger number of patients, to validate the correlation with Thyroid status and to justify the trial of a small dose of anti-thyroid drug in asthenozoospermic patients.

  3. Field data analysis of boar semen quality.

    Science.gov (United States)

    Broekhuijse, M L W J; Feitsma, H; Gadella, B M

    2011-09-01

    This contribution provides an overview of approaches to correlate sow fertility data with boar semen quality characteristics. Large data sets of fertility data and ejaculate data are more suitable to analyse effects of semen quality characteristics on field fertility. Variation in fertility in sows is large. The effect of semen factors is relatively small and therefore impossible to find in smaller data sets. Large data sets allow for statistical corrections on both sow- and boar-related parameters. Remaining sow fertility variation can then be assigned to semen quality parameters, which is of huge interest to AI (artificial insemination) companies. Previous studies of Varkens KI Nederland to find the contribution to field fertility of (i) the number of sperm cells in an insemination dose, (ii) the sperm motility and morphological defects and (iii) the age of semen at the moment of insemination are discussed in context of the possibility to apply such knowledge to select boars on the basis of their sperm parameters for AI purposes.

  4. Controle biológico da murcha bacteriana do tomateiro, por Pseudomonas spp. fluorescentes

    OpenAIRE

    Peixoto, Ana Rosa

    1997-01-01

    Esta revisão bibliográfica teve como objetivo avaliar o potencial de antagonismo de espécies de Pseudomonas fluorescentes a Pseudomonas solanacearum, agente causal da murcha bacteriana do tomateiro. Devido a dificuldade encontrada nas estratégias utilizadas para o controle da Murcha Bacteriana por meio de métodos convencionais, alguns outros tem sido estudados, como o uso de microrganismos benéficos. As rizobactérias vem proporcionando solução viável a algumas doenças consideradas de difícil ...

  5. Etiologia e evolução das meningites bacterianas em centro de pediatria

    OpenAIRE

    Romanelli Roberta M.C.; Araújo Claudete A.; Boucinhas Fernando; Carvalho Inácio R.; Martins Nelson R.L.; Freire Heliane B.M.

    2002-01-01

    Objetivo: determinar a prevalência dos agentes etiológicos das meningites bacterianas em serviço de referência, no atendimento de doenças infecciosas para o estado de Minas Gerais, e verificar a resposta ao tratamento utilizado.Métodos: estudo descritivo em que foram incluídas todas as crianças com diagnóstico provável de meningite, admitidas na instituição no período de junho a novembro/99.Resultados: obteve-se 210 casos de meningite, sendo 111 casos de etiologia bacteriana (52,9%). Destes, ...

  6. A orbifloxacina no tratamento das cistites bacterianas em gatos domésticos

    OpenAIRE

    Reche Junior Archivaldo

    2005-01-01

    O presente estudo teve como objetivos, avaliar o uso da orbifloxacina nas cistites bacterianas de felinos, e seus possíveis efeitos colaterais, mormente a nefro e/ou hepatotoxicidade. Para tanto, foram colhidas amostras de urina de 53 gatos com históricos variados, mas com predisposição à infecção urinária. A cistite bacteriana foi comprovada, através de exame microbiológico, em 13 pacientes. No entanto, apenas 10 apresentaram infecção por agentes sensíveis a orbifloxacina. Esses 10 pacientes...

  7. INTERACCIONES ENTRE Gluconacetobacter diazotrophicus Y LA COMUNIDAD BACTERIANA ENDÓFITA DE LA CAÑA DE AZÚCAR

    Directory of Open Access Journals (Sweden)

    Marcia M. Rojas

    2007-01-01

    Full Text Available Gluconacetobacter diazotrophicus es una bacteria endófita de caña de azúcar, que se ha comprobado que es capaz de fijar cantidades considerables de nitrógeno y producir sustancias estimuladoras del crecimiento vegetal, lo cual puede ser de suma importancia en el mejoramiento de este cultivo sobre bases agroecológicas. En el presente trabajo se demuestran, por primera vez, las interacciones que esta especie establece con otros representantes de la comunidad bacteriana endófita de la caña de azúcar empleando el micrométodo en portaobjetos. G. diazotrophicus ejerce efecto antagonista sobre otros miembros de la comunidad, lo cual está influido por las cepas y el tiempo de crecimiento de la especie, que a su vez es estimulada por otras presentes en el interior de la caña de azúcar. Este hecho pudiera contribuir a potenciar la promoción del crecimiento que puede ejercer G. diazotrophicus en la caña de azúcar.

  8. Effects of seasons on some semen parameters and bacterial contamination of Awassi ram semen.

    Science.gov (United States)

    Azawi, O I; Ismaeel, M A

    2012-06-01

    The objectives of the present study were to determine the effects of season on some semen parameters and bacterial contamination of Awassi ram semen. Semen samples from six mature Awassi rams were used in this study. Semen collection was performed with artificial vagina every week, from September 2009 to October 2010. Volume, sperm concentration, mass motility, individual motility, percentage live sperm and sperm abnormalities were evaluated. Moreover, determination of viable bacterial count of the rams was also recorded weekly. Higher (p highest (p highest values and differed significantly (p highest value of the mean sperm acrosomal defects (13.33 ± 0.63%) was recorded in December. The highest value of the mean viable bacterial count (138.3 ± 21.6) was recorded in July (summer). A significant decrease (p bacterial count was observed from the middle of winter towards the end of spring. The lowest bacterial count was noted in January (60.5 ± 2.98). It could be concluded from the results of the present study that there is an effect of season on ram semen quality, and summer high temperature in northern Iraq has no effect on Awassi ram semen. There is a significant effect of season on bacterial count on Awassi ram semen.

  9. Evaluation of CD52 positive sperms in subfertile human semen samples: Is there any relationship with main semen parameters?

    Directory of Open Access Journals (Sweden)

    Roshanak Aboutorabi

    2014-01-01

    Conclusion: Our results showed that the correlation between CD52 labeling and sperm motility was negatively significant, but we did not observe any relation with other semen parameters, such as sperm normal morphology, sperm concentration, and semen viscosity.

  10. Semen quality in Peruvian pesticide applicators: association between urinary organophosphate metabolites and semen parameters

    Directory of Open Access Journals (Sweden)

    Gasco Manuel

    2008-11-01

    Full Text Available Abstract Background Organophosphates are broad class of chemicals widely used as pesticides throughout the world. We performed a cross-sectional study of associations between dialkylphosphate metabolites of organophosphates and semen quality among pesticide applicators in Majes (Arequipa, Peru. Methods Thirty-one men exposed to organophosphate (OP pesticides and 31 non-exposed were recruited (age, 20–60 years. In exposed subjects, semen and a blood sample were obtained one day after the last pesticide application. Subjects were grouped according to levels of OP metabolites in urine. Semen samples were analyzed for sperm concentration, percentage of sperm motility, percentage of normal morphology, semen leucocytes and concentrations of fructose and zinc. Exposure to OP was assessed by measuring six urinary OP metabolites (dimethyl and diethyl phosphates and thiophosphates by gas chromatography using a single flame photometric detector. Results Diethyldithiophosphate (p = 0.04 and diethylthiophosphate (p = 0.02 better reflected occupational pesticide exposure than other OP metabolites. Semen analysis revealed a significant reduction of semen volume and an increase in semen pH in men with OP metabolites. Multiple regression analysis showed that both occupational exposure to pesticides and the time of exposure to pesticides were more closely related to alterations in semen quality parameters than the single measurement of OP metabolites in urine. Conclusion The study demonstrated that occupational exposure to OP pesticides was more closely related to alterations in semen quality than a single measurement of urine OP metabolites. Current measurement of OP metabolites in urine may not reflect the full risk.

  11. Urethral anatomy and semen flow during ejaculation

    Science.gov (United States)

    Kelly, Diane

    2016-11-01

    Ejaculation is critical for reproductive success in many animals, but little is known about its hydrodynamics. In mammals, ejaculation pushes semen along the length of the penis through the urethra. Although the urethra also carries urine during micturition, the flow dynamics of micturition and ejaculation differ: semen is more viscous than urine, and the pressure that drives its flow is derived primarily from the rhythmic contractions of muscles at the base of the penis, which produce pulsatile rather than steady flow. In contrast, Johnston et al. (2014) describe a steady flow of semen through the crocodilian urethral groove during ejaculation. Anatomical differences of tissues associated with mammalian and crocodilian urethral structures may underlie these differences in flow behavior.

  12. Chronic boron exposure and human semen parameters.

    Science.gov (United States)

    Robbins, Wendie A; Xun, Lin; Jia, Juan; Kennedy, Nola; Elashoff, David A; Ping, Liu

    2010-04-01

    Boron found as borates in soil, food, and water has important industrial and medical applications. A panel reviewing NTP reproductive toxicants identified boric acid as high priority for occupational studies to determine safe versus adverse reproductive effects. To address this, we collected boron exposure/dose measures in workplace inhalable dust, dietary food/fluids, blood, semen, and urine from boron workers and two comparison worker groups (n=192) over three months and determined correlations between boron and semen parameters (total sperm count, sperm concentration, motility, morphology, DNA breakage, apoptosis and aneuploidy). Blood boron averaged 499.2 ppb for boron workers, 96.1 and 47.9 ppb for workers from high and low environmental boron areas (pBoron concentrated in seminal fluid. No significant correlations were found between blood or urine boron and adverse semen parameters. Exposures did not reach those causing adverse effects published in animal toxicology work but exceeded those previously published for boron occupational groups.

  13. Ejaculatory process and related semen characteristics.

    Science.gov (United States)

    Bravo, P W; Moscoso, R; Alarcon, V; Ordoñez, C

    2002-01-01

    South American camelids are dribble ejaculators, and urethral contractions occur throughout copulation, which may last 25 min. The urethral contractions and their association with semen characteristics during copulation were determined in llamas and alpacas. A transrectal probe was held in the rectum of the male while copulating an artificial vagina, which was accessed underneath the dummy through a hole. The semen-collecting tube was changed every 5 min. Semen characteristics, color, volume, consistency, motility, concentration, and percentage of live sperm were determined at 5-min intervals. Urethral contractions were evenly distributed during copulation: 40 in alpacas and 63 in llamas (p < .05), with a general range of 11 to 132. Semen color was milky in 63%, and translucent in 36.5% for alpacas; and creamy (9.9%), milky (47%), and translucent (42%) for llamas. The mean volume of ejaculate was 0.3, 0.4, 0.6, 0.7, 0.6, 0.8, 0.3, and 3.0 mL for 5, 10, 15, 20, 25, and 30 min, respectively. Semen consistency was variable: viscous (65%) and semiviscous (34%) in alpacas; and viscous (57%) and semiviscous (42%) in llamas. Spermatic motility varied between 60 and 80% for the llama, and 40 and 80% for the alpaca. Spermatic concentration varied between 60 and 188 x 10(3)/mm3 in llamas, and 30 and 170 x 10(3)/mm3 in alpacas. Percentage of live sperm varied the least: 81 to 90% in llamas and 65 to 90% in alpacas. The ejaculate of llamas and alpacas is not fractionated, urethral contractions are evenly distributed, during copulation, and semen characteristics are present throughout the copulatory period.

  14. Effect of alternate day collection on semen quality of Asian elephants (Elephas maximus) with poor initial fresh semen quality

    NARCIS (Netherlands)

    Imrat, P.; Mahasawangkul, Sittidet; Thitaram, Chatchote; Suthanmapinanth, P.; Kornkaewrat, K.; Sombutputorn, P.; Jansittiwate, S.; Thongtip, Nikorn; Pinyopummin, A.; Colenbrander, B.; Holt, W.V.; Stout, Tom

    2014-01-01

    In captivity, male Asian elephants often yield poor quality semen after transrectal manually assisted semen collection; however, the reasons for the disappointing semen quality are not clear. Here we test the hypothesis that accumulation of senescent spermatozoa is a contributory factor, and that se

  15. USO DE PATRONES DE DIFRACCIÓN DE LUZ LÁSER Y ANÁLISIS DE IMAGENES PARA DIFERENCIACIÓN DE TRES TIPOS DE COLONIAS BACTERIANAS

    Directory of Open Access Journals (Sweden)

    GLORIA ARANGO

    2013-01-01

    Full Text Available La diferenciación rápida de colonias bacterianas es esencial en muchos campos industriales, científicos y de salud humana y animal. Generalmente esa diferenciación se logra por técnicas microscópicas, bioquímicas o genéticas muy elaboradas que requieren tiempo y entrenamiento. En este estudio se utilizaron técnicas de análisis de imágenes sobre patrones de difracción de luz láser para evaluar su sensibilidad en la diferenciación de colonias bacterianas. Adicionalmente se estudió el efecto del tiempo de crecimiento de las colonias en la generación de estos patrones y se determinó la influencia de la cantidad de medio de cultivo en la difracción de luz láser. Los patrones de difracción del láser se obtuvieron sobre cultivos puros de cinco aislamientos bacterianos B1, B2, B3, B2Ca y B2Cb. En la primera prueba de diferenciación se utilizaron colonias de B2, B3, B2Ca. En el estudio del efecto del tiempo de crecimiento sobre patrones de difracción, las bacterias B1 y B2Cb con uno o dos días de crecimiento. Finalmente, el efecto de la cantidad de medio de cultivo en la difracción de la luz, se evaluó en una prueba en cajas petri con 10, 15 o 20 ml de medio de cultivo estéril sin colonias de bacterias. Mediante un análisis de escalado multidimensional realizado con los parámetros de textura extraídos de las imágenes de los patrones, se obtuvo un agrupamiento adecuado de los patrones asociados a las colonias de cada bacteria evaluada, lo cual indicó que las colonias bacterianas se pueden diferenciar por sus patrones de difracción. Adicionalmente se encontró que no hay variaciones significativas en los patrones de difracción obtenidos de dos aislamientos bacterianos en dos tiempos de crecimiento. El espesor del medio de cultivo afectó la difracción del haz de luz pues patrones obtenidos de cajas petri con 10 ml se separan completamente de los patrones obtenidos con 15 y 20 ml, aunque no hay diferencias entre los dos

  16. EVALUACIÓN DE ETILENGLICOL COMO CRIOPROTECTOR EN LA CRIOCONSERVACIÓN DE SEMEN DE BAGRE BLANCO (Sorubim cuspicaudus, PIMELODIDAE

    Directory of Open Access Journals (Sweden)

    VÍCTOR J. ATENCIO-GARCÍA

    2014-01-01

    Full Text Available Se evaluó el semen crioconservado de Sorubim cuspicaudus utilizando etilenglicol (ETG a tres niveles de inclusión (5, 10, 15 %. Machos (n = 13 en fase de espermiación y hembras (n = 6 en maduración final se indujeron con 0,4 ml de Ovaprim®/Kg, después de 12 a 14 horas post-inducción se colectó el semen en viales Eppendorf de 2 ml de capacidad. Las diferentes soluciones crioprotectoras se prepararon con glucosa 6 % (p/v, leche en polvo descremada 5 % (pv y agua destilada. El semen fue diluido en proporción 1:3 (semen:diluyente, empacado en macrotubos de 2,5 ml y congelado en vapores de nitrógeno líquido (NL durante 30 minutos y luego almacenados en termos criogénicos sumergidos directamente en NL (-196 °C. El semen crioconservado fue descongelado en baño serológico a 35 °C durante 90 segundos. La movilidad total, progresividad y velocidad espermática del semen fresco y descongelado se analizó con el software Sperm Class Analizer SCA® (Microptic SL, España. La fertilidad y eclosión se evaluó con 1,0-1,5 g de ovocitos en incubadoras experimentales de flujo ascendente de dos litros de capacidad. Se utilizó un diseño completamente aleatorizado. El semen fresco registró tasa de eclosión de 51,8±21 %, sin observarse diferencia significativa con la obtenida con el semen crioconservado con ETG 5 % (38,6 ± 13,9 % (p> 0,05; mientras que ETG 15 % (9,6 ± 2,9 % reportó la menor eclosión (p <0,05. Los resultados sugieren que la solución crioprotectora compuesta por ETG 5 %, glucosa 6 % y leche en polvo 5 % es una alternativa viable para la criocon- servación de semen de Sorubim cuspicaudus con fecundaciones similares al usar semen fresco.

  17. Recognition of MIR Data of Semen Armeniacae Amarum and Semen Persicae Using Discrete Wavelet Transformation and BP-Artificial Neural Network

    OpenAIRE

    Cheng, Cun-Gui; Yu, Peng; Wu, Chang-Shun; Shou, Jia-Ni

    2012-01-01

    Horizontal attenuation total reflection-Fourier transformation infrared spectroscopy (HATR-FT-IR) is used to measure the Mid-IR (MIR) of semen armeniacae amarum and its confusable varieties semen persicae. In order to extrude the difference between semen armeniacae amarum and semen persicae, discrete wavelet transformation (DWT) is used to decompose the MIR of semen armeniacae amarum and semen persicae. Two main scales are selected as the feature extracting space in the DWT domain. According ...

  18. Impact of Organophosphorous Pesticide Exposure on Semen Quality

    Institute of Scientific and Technical Information of China (English)

    Wen-ying LI; Jun-qing WU; Xiao-ping ZOU; Cai-qin XIAO; Wei-jin ZHOU; Er-sheng GAO

    2003-01-01

    Objective To investigate the impact of organophorous pesticide exposure on semen quality and other risk factors of semen qualityMethod Questionnaire investigation, external genital examination and laboratory examination of semen quality were conducted on 322 subjects, who were divided into exposed and control group, each consisting of 161 subjects. Results Multivariate analysis showed that educational level and years of alcohol drinking had effect on semen volume. Compared with subjects with primary school education or lower, those with junior high school education had higher value of semen volume(OR=1.961). Proportion of subjects with high semen volume decreased with the increasing years of alcohol drinking (OR=0.962). Organophosphorous pesticide exposure could result in the decline of the number of sperms with progressive forward progression (OR=0.528), figure of semen density (OR=0.266), semen viability (OR=0.398) and percentage of normal sperm morphology (OR=0.281). There are possible relationships between the season of semen collection and the number of sperms with progressive forward progression, semen viability and percentage of normal sperm morphology. Compared to summer, values of the above three indices would be higher in winter (OR was 2.272, 4.060 and 5.249, respectively).Conclusion Exposure to organophosphorous pesticide could result in the deterioration of semen quality of the peasants.

  19. Semen analysis and sperm function testing

    Institute of Scientific and Technical Information of China (English)

    Daniel R Franken; Sergio Oehninger

    2012-01-01

    Despite controversy regarding the clinical value of semen analysis,male fertility investigation still relies on a standardized analysis of the semen parameters.This is especially true for infertility clinics in both developing and developed countries.Other optional tests or sophisticated technologies have not been widely applied.The current review addresses important changes in the analysis of semen as described in the new World Health Organization (WHO) manual for semen analysis.The most important change in the manual is the use of evidence-based publications as references to determine cutoff values for normality.Apart from the above mentioned changes,the initial evaluation and handling methods remain,in most instances,the same as in previous editions.Furthermore,the review evaluates the importance of quality control in andrology with emphasis on the evaluation of sperm morphology.WHO sperm morphology training programmes for Sub-Saharan countries were initiated at Tygerberg Hospital in 1995.1he external quality control programme has ensured that the majority of participants have maintained their morphological reading skills acquired during initial training.This review reports on current sperm functional tests,such as the induced acrosome reaction,and sperm-zona pellucida binding assays,as well as the impact of sperm qualitv in terms of DNA integritv,and the relationship of sperm function tests to sperm morphologv.

  20. Semen quality improves marginally during young adulthood

    DEFF Research Database (Denmark)

    Perheentupa, Antti; Sadov, Sergey; Rönkä, Riitta

    2016-01-01

    in several studies. The longitudinal development of semen quality in early adulthood is insufficiently understood. STUDY DESIGN, SIZE, DURATION: A longitudinal follow-up of two cohorts of volunteer young adult Finnish men representing the general population was carried out. Cohorts A (discovery cohort, born...

  1. Métodos de coleccion de semen en camélidos sudamericanos - Methods of semen collection in south american camelids

    Directory of Open Access Journals (Sweden)

    Pacheco Curie, Joel Iván;

    2008-04-01

    Full Text Available ResumenLa colección de semen depende de una buena y constante producciónespermática para que la calidad del semen sea buena. Las técnicas decolección de semen están bastante desarrolladas en otros animales,especialmente en rumiantes domésticos en los cuales ya es unprocedimiento de rutina, pero en camélidos, dadas las especialescaracterísticas reproductivas, anatómicas y fisiológicas de estasespecies, esta colección es bastante dificultosa y no existe un protocolo recomendado y una técnica optima, así como su manejo posterior. Las características seminales son también muy variables y dependen de la forma de colección y existen varios factores que afectan su calidad, así como frecuencia de colección, edad, época, etc., por lo que también se evaluaron y desarrollaron diferentes técnicas de degelificar, diluir, conservar y congelar estas células espermáticas, de acuerdo a la técnica de colección y la especie, así como la utilización de dilutores y crioprotectores utilizados para otras especies, pudiéndose posteriormente utilizar en la evaluación reproductiva de los machos.SummaryThe collection of semen depends on a good and constant spermaticproduction so that the quality of the semen is good. The techniques ofcollection of semen enough developed in other animals are, especially in ruminant domestic in which is already a routine procedure, but incamélids, given the special ones characteristic reproductive, anatomical and physiologic of these species, this collection is quite difficult and it doesn't exist a recommended protocol and a good technique, as well as its later handling. The seminal characteristics are also very variable and they depend in the collection way and several factors that affect their quality, exist as well as collection frequency, age, time, etc, for what too were also evaluated and they developed different techniques of degelification, to dilute, to conserve and to freeze these spermatic cells

  2. Cadherina epitelial como biomarcador de espermatozoides humanos funcionales. Estudios realizados en muestras de semen fresco y previamente criopreservado de donantes y de pacientes en tratamiento por infertilidad

    OpenAIRE

    Vazquez, Monica Hebe; Veiga, Maria Florencia; Marin Briggiler, Clara Isabel; Valcarcel, Alberto

    2015-01-01

    Las técnicas de reproducción asistida son procedimientos estándar para el tratamiento de la infertilidad, si bien, en algunos casos, los resultados aún son adversos. Las alteraciones en el hombre contribuyen a esta patología en hasta 50% de los casos. Para la terapéutica se emplean espermatozoides del semen obtenidos en el momento del procedimiento, aunque para ciertos pacientes y para todos los donantes, se emplea semen previamente criopreservado que produce injurias en la gameta. El monitor...

  3. A comparison of ABAcard(®) p30 and RSID™-Semen test kits for forensic semen identification.

    Science.gov (United States)

    Boward, Emily S; Wilson, Stacey L

    2013-11-01

    The screening and confirmatory tests available to a forensic laboratory allow evidence to be examined for the presence of bodily fluids. With the majority of evidence being submitted involving sexual assaults, it is important to have confirmatory tests for the identification of semen that are straightforward, quick, and reliable. The purpose of this study was to compare two commonly used semen identification kits utilized by forensic laboratories: ABAcard(®) p30 and Rapid Stain Identification of Human Semen (RSID™-Semen). These kits were assessed with aged semen stains, fresh and frozen post-vasectomy semen, post-coital samples collected on different substrates, post-vasectomy semen mixed with blood, saliva, and urine, a series of swabs collected at increasing time intervals after sexual intercourse, and multiple non-semen samples. The test kits were compared on the basis of sensitivity, specificity, and the cost and time effectiveness of each protocol. Overall, both semen identification tests performed well in the studies. Both kits proved specificity for identifying semen, however the ABAcard(®) p30 test surpassed the RSID™-Semen test in sensitivity, cost per test, and simplified test protocol.

  4. Tratamiento de agrandamiento gingival inducido por fenitoina asociado a placa bacteriana

    Directory of Open Access Journals (Sweden)

    Carmen Julia Rovira

    2013-10-01

    Full Text Available ResumenLa ingesta de algunos medicamentos puede producir un sobre crecimiento de las encías. Se reportan medicamentos tales como los bloqueadores de los canales de calcio, inmunosupresores, anticonvulsivantes que producen estos efectos adversos. Múltiples casos de agrandamientos gingivales están asociados a la ingesta de Fenitoína. El tratamiento de los agrandamientos gingivales inducidos por drogas por lo general es quirúrgico, pero mantener un buen control de placa bacteriana puede evitar en muchos casos que se presente un exagerado aumento de volumen gingival. Se presenta un caso de agrandamiento gingival en paciente masculino de 22 años de edad, bajo tratamiento antiepiléptico con fenitoína. Al examen clínico se observó aumento del volumen gingival de forma generalizada, en maxilar superior e inferior, predominante en sector anterior, produciendo ardor y sangrado durante el cepillado y la ingestión de alimentos. Al sondaje presenta múltiples pseudobolsas en los diferentes cuadrantes. La radiografía panorámica reveló pérdida ósea moderada horizontal, predominante en el sector posterosuperior, posteroinferior y anteroinferior. El plan de tratamiento consistió en fase higiénica, instrucción y motivación en higiene oral a la madre, gingivectomía y gingivoplastía en ambos maxilares. (DUAZARY 2011 No. 2, 226 - 231Abstract The importance of endodontic treatment is to achieve a complete filling and sealing of the root canal to prevent the transport of contaminants to and from root canal, with materials compatible with the surrounding tissues. Occasionally a situation may arise in which the purpose of the maintenance of working length is compromised such as overenlargement. This situation allows the extrusion of filling materials, mainly sealer cements, causing post treatment complications such as inflammatory reactions, injury to adjacent structures (orbital pain, inferior alveolar nerve injury, headache, etc., and infections

  5. Medición de cambios cuantitativos de la microbiota subgingival posterior a la remoción de placa bacteriana supragingival Measurement of quantitative changes of the microbiota subgingival after to removal of bacterial plaque supragingival

    OpenAIRE

    C. Godoy; Melej,C; Silva, N

    2010-01-01

    Uno de los campos de interés en el estudio de la microbiología periodontal para muchos investigadores ha sido identificar si es posible que los microorganismos responsables del origen y progresión de la enfermedad periodontal que habitan sobre el margen gingival (supragingival) y bajo este (subgingival) tengan una relación directa que permita mantener interacciones influyentes en el crecimiento y desarrollo de las diferentes especies bacterianas que habitan en los tejidos periodontales. Por l...

  6. Bacterial diversity in the sediments of a temperate artificial lake, Rapel reservoir Diversidad bacteriana en sedimentos de un lago artificial temperado, embalse Rapel

    Directory of Open Access Journals (Sweden)

    CRISTINA DORADOR

    2007-06-01

    Full Text Available Rapel reservoir is an eutrophic system in Chile that has undergone a series of anthropogenic impacts in recent decades. To provide a better understanding of the processes occurring in the reservoir, we examined bacterial composition from surface sediments using traditional microbiology techniques and molecular biology tools. Our results showed significant temporal variation in the physical and chemical composition of the water column, but no depth-related differences during the study period. To detect temporal changes in bacterial composition, cultivable heterotrophic bacteria, heterotrophic iron oxidizing bacteria, and sulfate-reducing bacteria were extracted from the surface sediments and their concentration measured. Microbial diversity in sediments was represented by closest relatives of eight different bacterial phyla. The most frequently recovered phylotypes in the clone library of 16S rDNA were related to sulfate-reducing bacteria belong to the Deltaproteobacteria groupEl embalse Rapel es un sistema eutrófico en Chile que ha experimentado diversos impactos antropogénicos en las últimas décadas. Para conocer mejor los posibles procesos que ocurren en el embalse, la composición bacteriana fue estudiada en la zona superficial de los sedimentos utilizando técnicas microbiológicas tradicionales y herramientas de biología molecular. Nuestros resultados mostraron variación temporal significativa en la composición física y química del agua, no encontrándose diferencias de las variables medidas entre superficie y fondo en la columna de agua durante el período de estudio. Para detectar cambios temporales en la concentración y composición bacteriana, se midieron bacterias heterótrofas, heterótrofas oxidantes de fierro y bacterias reductoras de sulfato extraídas de la superficie de los sedimentos. La diversidad bacteriana en los sedimentos estuvo representada por 8 diferentes Phyla del dominio Bacteria. Los filotipos m

  7. Evaluación de dimetilacetamida como crioprotector para la crioconservación de semen de bocachico Prochilodus magdalenae

    Directory of Open Access Journals (Sweden)

    VJ Atencio

    2013-01-01

    Full Text Available Se evaluó dimetilacetamida (DMA como crioprotector, a tres concentraciones: 8% (0,85 M, 10% (1,07 M y 12% (1,29 M combinado con glucosa 6% (0.33 M y yema de huevo 12% para crioconservar semen de bocachico Prochilodus magdalenae. El semen fue diluido en proporción 1:4 en la solución crioprotectora, empacado en pajuelas de 2,5 mL y congelado en vapores de nitrógeno líquido (N2L durante 30 minutos y luego almacenados en un termo de N2L de 34 L. La concentración, movilidad total, progresividad y velocidad tanto en semen fresco (control como en semen crioconservado-descongelado se evaluó con ayuda de Sperm Class Analyzer (SCA. Las pajuelas fueron descongeladas en baño serológico a 60°C durante 45 segundos. La fertilidad y eclosión fueron evaluadas inseminando dos gramos de huevos (1540 huevos/g a razón de 320.000 spz/ovocito. El semen fresco registró mayores valores de movilidad total, progresividad total, velocidad curvilínea y velocidad lineal con respecto al semen crioconservado-descongelado (P 0,05. DMA 12% reportó los menores valores de todas las variables que evaluaron la calidad seminal. Con semen fresco se obtuvo fertilidad de 74,0 ± 5,3% y eclosión de 62,3 ± 3,1%; mientras que con semen crioconservado-descongelado con DMA 8% se obtuvo adecuada fertilidad (60,4 ± 8,4% y eclosión (50,1 ± 9,8%. Los resultados del estudio sugieren que la solución crioprotectora compuesta por DMA 8%, glucosa 6% y yema de huevo 12% es una alternativa viable para la crioconservación de semen de bocachico.

  8. QUIMIOTAXIS BACTERIANA Y FLAVONOIDES: PERSPECTIVAS PARA EL USO DE PROBIOTICOS.

    OpenAIRE

    Mónica Marcela Galicia-Jiménez; Carlos Sandoval-Castro; Rafael Rojas-Herrera; Héctor Magaña-Sevilla

    2011-01-01

    La aplicación de diversas dietas se han enfocado en buscar mayor producción en los rumiantes variando el alimento del animal, afectando así la población microbiana ruminal, sin embargo, la eficacia en la producción del rumiante ha sido reportada de manera inconsistente. Por otro lado, se han realizado estudios de metabolitos secundarios secretados por plantas y se ha visto que tienen una función de atrayente o repelente en la interacción planta-microorganismo. Por lo que nuestro objetivo es i...

  9. Semen phthalate metabolites, semen quality parameters and serum reproductive hormones: A cross-sectional study in China.

    Science.gov (United States)

    Wang, Yi-Xin; Zeng, Qiang; Sun, Yang; Yang, Pan; Wang, Peng; Li, Jin; Huang, Zhen; You, Ling; Huang, Yue-Hui; Wang, Cheng; Li, Yu-Feng; Lu, Wen-Qing

    2016-04-01

    Exposure to phthalates has been found to have adverse effects on male reproductive function in animals. However, the findings from human studies are inconsistent. Here we examined the associations of phthalate exposure with semen quality and reproductive hormones in a Chinese population using phthalate metabolite concentrations measured in semen as biomarkers. Semen (n = 687) and blood samples (n = 342) were collected from the male partners of sub-fertile couples who presented to the Reproductive Center of Tongji Hospital in Wuhan, China. Semen quality parameters and serum reproductive hormone levels were determined. Semen concentrations of 8 phthalate metabolites were assessed using high-performance liquid chromatography and tandem mass spectrometry. Associations of the semen phthalate metabolites with semen quality parameters and serum reproductive hormones were assessed using confounder-adjusted linear and logistic regression models. Semen phthalate metabolites were significantly associated with decreases in semen volume [mono-n-butyl phthalate (MBP), mono-(2-ethylhexyl) phthalate (MEHP), mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP), mono(2-ethyl-5-oxohexyl) phthalate (MEOHP)], sperm curvilinear velocity [monobenzyl phthalate (MBzP), MEHP, the percentage of di-(2-ethylhexyl)-phthalate metabolites excreted as MEHP (%MEHP)], and straight-line velocity (MBzP, MEHP, %MEHP), and also associated with an increased percentage of abnormal heads and tails (MBzP) (all p for trend hormones. Our findings suggest that environmental exposure to phthalates may impair human semen quality.

  10. SIFAT FISIKA DAN KETEGUHAN PATAH PAPAN SEMEN PARTIKEL DARI PELEPAH KELAPA SAWIT (Elaeis guineensis Jack

    Directory of Open Access Journals (Sweden)

    Violet Burhanuddin

    2016-08-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui kemungkinan pemanfaatan pelepah kelapa sawit sebagai bahan baku papan semen partikel dan untuk mengetahui sifat fisika dan mekanika papan semen partikel pada berbagai perbandingan semen dan partikel dari dua merek semen (Tonasa dan Tiga Roda. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL dengan pola faktorial 2 x 3 sebanyak 5 kali ulangan, dimana faktor A adalah merek semen yaitu semen tonasa dan semen Tiga Roda kemudian faktor B adalah perbandingan antara partikel dan semen. Hasil pengujian sifat fisika dan mekanika masing-masing menunjukan pengaruh yang sangat berbeda nyata, hal ini disebabkan karena semakin banyak penambahan jumlah perekat semen maka semakin baik pula sifat fisika dan mekanika papan semen yang dibuat, sedangkan untuk faktor merek semen tidak menunjukan perbedaan yang nyata. Kata kunci : Pelapah kelapa sawit, sifat fisika dan mekanika, papan semen partikel

  11. Utilization of Frozen Semen of Wild Yak

    Institute of Scientific and Technical Information of China (English)

    阎萍

    2005-01-01

    After domestication of wild yak and utilization of its frozen semen was successful in 1983, frozen semen of wild yak was used to improve domestic yak and local yellow cattle by artificial insemination(AI). Hybrid vigor of their F1 was obvious, i. e. , productive performance of F1 was significantly increased. Their offspring did not only have significant heterosis in performance but also can rejuvenate effectively their adaptability and survival, and can use alpine grassland more efficiently. This resulted in significant social and economic benefits.Compared with dairy cattle, beef cattle and yellow cattle, AI of yak was more difficult. Using AI to improve yak performance was difficult and significant in yak production areas of our country. It is necessary to invest more technique and fund to extend AI.

  12. ALKALINE PHOSPHATASE ACTIVITY AS A MARKER OF DOG SEMEN FREEZABILITY

    Directory of Open Access Journals (Sweden)

    KOSINIAK-KAMYSZ K.

    2007-01-01

    Full Text Available The investigation was performed to evaluate the dog semen freezability and itsquality after thawing allowing its use for artificial insemination (AI. On the basis ofsperm motility, concentration and alkaline phosphatase (AP activity in semenplasma it was possible to establish that AP activity corresponds with the basic factorof semen examination. Significant statistical differences occurred between thequality of ejaculates which were qualified or disqualified to deep freezing and AI.These results show that AP activity in raw dog semen plasma can be used as amarker for the dog semen qualification for deep freezing and AI with 95%probability of the prognosis of the results.

  13. Uranium quantification in semen by inductively coupled plasma mass spectrometry.

    Science.gov (United States)

    Todorov, Todor I; Ejnik, John W; Guandalini, Gustavo; Xu, Hanna; Hoover, Dennis; Anderson, Larry; Squibb, Katherine; McDiarmid, Melissa A; Centeno, Jose A

    2013-01-01

    In this study we report uranium analysis for human semen samples. Uranium quantification was performed by inductively coupled plasma mass spectrometry. No additives, such as chymotrypsin or bovine serum albumin, were used for semen liquefaction, as they showed significant uranium content. For method validation we spiked 2g aliquots of pooled control semen at three different levels of uranium: low at 5 pg/g, medium at 50 pg/g, and high at 1000 pg/g. The detection limit was determined to be 0.8 pg/g uranium in human semen. The data reproduced within 1.4-7% RSD and spike recoveries were 97-100%. The uranium level of the unspiked, pooled control semen was 2.9 pg/g of semen (n=10). In addition six semen samples from a cohort of Veterans exposed to depleted uranium (DU) in the 1991 Gulf War were analyzed with no knowledge of their exposure history. Uranium levels in the Veterans' semen samples ranged from undetectable (<0.8 pg/g) to 3350 pg/g. This wide concentration range for uranium in semen is consistent with known differences in current DU body burdens in these individuals, some of whom have retained embedded DU fragments.

  14. Oftalmoplegia internuclear achado clínico inicial e único em endocardite bacteriana

    Directory of Open Access Journals (Sweden)

    Antonio L. dos Santos Werneck

    1990-03-01

    Full Text Available Oftalmoplegia internuclear unilateral traduz-se por paralisia homolateral da adução ocular e nistagmo contralateral. As principais causas são a esclerose múltipla e a insuficiência vértebro-basilar. Apresentamos um caso secundário a endocardite bacteriana, em que este sinal iniciou o quadro e foi o único achado neurológico.

  15. Tratamiento de agrandamiento gingival inducido por fenitoina asociado a placa bacteriana

    OpenAIRE

    Carmen Julia Rovira; Jennifer Orozco Páez; Antonio Díaz Caballero

    2013-01-01

    ResumenLa ingesta de algunos medicamentos puede producir un sobre crecimiento de las encías. Se reportan medicamentos tales como los bloqueadores de los canales de calcio, inmunosupresores, anticonvulsivantes que producen estos efectos adversos. Múltiples casos de agrandamientos gingivales están asociados a la ingesta de Fenitoína. El tratamiento de los agrandamientos gingivales inducidos por drogas por lo general es quirúrgico, pero mantener un buen control de placa bacteriana puede evitar e...

  16. Caracterización de pacientes con bronconeumonía bacteriana asociada a la ventilación artificial mecánica

    Directory of Open Access Journals (Sweden)

    Danilo Eranio Utra Cardoso

    2015-06-01

    Full Text Available Fundamento: las neumonías nosocomiales constituyen hoy en día un problema de salud a nivel mundial. En las unidades de cuidados intensivos de las instituciones hospitalarias ingresa un elevado número de pacientes que requieren ventilación artificial mecánica, factor de riesgo asociado a este tipo de infecciones. Objetivo: caracterizar a los pacientes con neumonías bacterianas asociadas a la ventilación artificial mecánica, ingresados en la sala de cuidados intensivos del Hospital General Docente “Dr. Ernesto Guevara de la Serna”, Las Tunas, Cuba; durante el año 2012. Métodos: se realizó un estudio descriptivo, retrospectivo, en los pacientes (262 ingresados en esa unidad, en el período de enero a diciembre de 2012. La muestra quedó conformada por los 173 pacientes complicados por neumonías bacterianas. Los datos fueron tomados de los expedientes clínicos. Se midieron las variables: grupos de edad, manifestaciones radiológicas, gérmenes más frecuentes y modalidades ventilatorias empleadas. Resultados: predominaron los pacientes mayores de 60 años, para un 41 %, seguido por el grupo de 50 a 59 años con 26,5 %. El germen más frecuente fue el bacilo no fermentador para el 76,6 %; la modalidad ventilatoria de volumen control fue la más utilizada, en 75 pacientes (43,6 %; el trastorno hidromineral se presentó en el 41 % de los casos, todos con tendencia a la hipokaliemia, que dio lugar a un difícil destete; los síntomas radiológicos anatomoclínicos fueron los relacionados con las neumonías a focos diseminados con 95 pacientes para 54,9 %. Conclusiones: se logró caracterizar a los pacientes con neumonías bacterianas asociadas a la ventilación artificial mecánica.

  17. Recognition of FT-IR Data Cuscutae Semen, Japanese Dodder, and Sinapis Semen Using Discrete Wavelet Transformation and RBF Networks

    Directory of Open Access Journals (Sweden)

    Tao Hu

    2013-01-01

    Full Text Available Horizontal attenuation total reflection Fourier transformation infrared spectroscopy (HATR-FT-IR studies on cuscutae semen and its confusable varieties Japanese dodder and sinapis semen combined with discrete wavelet transformation (DWT and radial basis function (RBF neural networks have been conducted in order to classify them. DWT is used to decompose the FT-IRs of cuscutae semen, Japanese dodder, and sinapis semen. Two main scales are selected as the feature extracting space in the DWT domain. According to the distribution of cuscutae semen, Japanese dodder, and sinapis semen’s FT-IRs, three feature regions are determined at detail 3, and two feature regions are determined at detail 4 by selecting two scales in the DWT domain. Thus five feature parameters form the feature vector. The feature vector is input to the RBF neural networks to train so as to accurately classify the cuscutae semen, Japanese dodder, and sinapis semen. 120 sets of FT-IR data are used to train and test the proposed method, where 60 sets of data are used to train samples, and another 60 sets of FT-IR data are used to test samples. Experimental results show that the accurate recognition rate of cuscutae semen, Japanese dodder, and sinapis semen is average of 100.00%, 98.33%, and 100.00%, respectively, following the proposed method.

  18. Fermentation Tecniques and Applications of Bacterial Cellulose: a Review Técnicas de fermentación y aplicaciones de la celulosa bacteriana: una revisión

    Directory of Open Access Journals (Sweden)

    Luz Dary Carreño Pineda

    2012-12-01

    Full Text Available Bacterial cellulose is a polymer obtained by fermentation with microorganismsfrom Acetobacter, Rhizobium, Agrobacterium and Sarcina genera. Amongthem, Acetobacter xylinum is the most efficient specie. This polymer hasthe same chemical composition of plant cellulose, but its conformation andphysicochemical properties are different, making it attractive for several applications, especially in the areas of food, separation processes, catalysis andhealth, due to its biocompatibility. However, the main problem is the production in mass that is constrained by low yield. It is therefore necessaryto develop some alternatives. This paper presents a review about synthesis,production, properties and principal applications of bacterial cellulose, as wellas some alternatives to reduce the difficulties for process scaling.La celulosa bacteriana es un polímero obtenido por fermentación con microrganismosde los géneros Acetobacter, Rhizobium, Agrobacterium y Sarcina, delas cuales la especie más eficiente es la Acetobacter Xylinum. Este polímero presenta la misma estructura química de la celulosa de origen vegetal, pero difiereen su conformación y propiedades fisicoquímicas, lo que lo hace atractivo para diversas aplicaciones, especialmente en las áreas de alimentos, procesosde separación, catálisis y en medicina, gracias a su biocompatibilidad. Sin embargo, el principal problema es la producción a gran escala limitada por losbajos rendimientos, lo que genera la necesidad de desarrollar alternativas que permitan disminuir o eliminar las causas de esta limitación. En este artículo se hace una revisión acerca de la síntesis, producción, propiedades y principales aplicaciones de la celulosa bacteriana, así como de algunas alternativas estudiadas para disminuir los inconvenientes en el escalamiento del proceso.

  19. POBLACIONES BACTERIANAS NATIVAS: ALTERNATIVA SUSTENTABLE PARA LA AGRICULTURA

    OpenAIRE

    Lina Hernández-Flores; J. Antonio Munive-Hernández; Engelberto Sandoval-Castro; Daniel Martínez-Carrera; Ma. Carmen Villegas-Hernández

    2012-01-01

    La producción agrícola moderna requiere gran cantidad de agroquímicos derivados del petróleo, por lo que su producción y uso contaminan el ambiente, además de generar daños en la salud de los seres vivos. Una alternativa para mantener un nivel rentable de producción, con un menor uso de agroquímicos, y enfocando la agricultura hacia un manejo sustentable, es la aplicación de microorganismos fijadores de nitrógeno. De esta forma, se busca implementar alternativas que permitan reducir los costo...

  20. Serum and semen zinc levels in normozoospermic and oligozoospermic men

    Energy Technology Data Exchange (ETDEWEB)

    Madding, C.I.; Jacob, M.; Ramsay, V.P.; Sokol, R.Z.

    1986-01-01

    We studied 11 unselected men who presented to a Reproductive Endocrinology Clinic with histories of infertility and low sperm counts. Reproductive hormones and semen und serum zinc levels were measured. All men had semen analyses performed on at least three separate occasions. A similar set of laboratory evaluations were performed on 11 other men who had normal semen analyses and no history of infertility. No abnormalities of reproductive hormones were found in either group. Mean serum zinc levels were significantly lower in the infertile men. Mean semen zinc levels were not significantly different. There was no correlation between serum and semen zinc levels in either group. A significant correlation was found between sperm count and semen zinc in the volunteers with normal counts, but not in the oligozoospermic men. The results obtained in this study suggest that lowered serum zinc is more common than formerly appreciated in unselected patients with infertility. The high level of zinc found in semen is due primarily to the secretions of the prostate gland and reflects prostatic stores. Serum zinc is thought to be a reasonable indicator of zinc status. The lack of correlation between serum zinc and semen zinc found in our study suggests that mild zinc deficiency may lower serum zinc while the larger prostatic zinc stores remain unaffected.

  1. AZF Microdeletions in Human Semen Infected with Bacteria

    Directory of Open Access Journals (Sweden)

    Hayfa H Hassani

    2011-11-01

    Full Text Available Bacterial infections are associated with infertility in men. This study was aimed to investigate microdeletions on Yq chromosome in semen infected with bacteria by using bacteriological, biochemical, and serological assays. The investigation showed that 107 of 300 (84.80% semen samples collected from infertile men with primary or secondary infertility were infected with different species of bacteria. Chlamydia trachomatis and Neisseria gonorrheae were the most frequently diagnosed bacteria in the infected semen samples. The percentages of infections of semen samples with C. trachomatis and N. gonorrhea were 42.31% and 35.28% respectively. Genomic DNA from each semen sample infected with predominant bacteria was analyzed for AZF deletions by using multiplex PCR. Different patterns of AZF microdeletions were obtained. It can be concluded that sexually transmitted bacteria may contribute in microdeletions of Yq chromosome by indirectly producing reactive oxygen species and causing gene defect in AZF regions.

  2. PATOGENICIDAD BACTERIANA EN MAÍZ (ZEA MAYS

    Directory of Open Access Journals (Sweden)

    Rocío Pérez-y-Terrón

    2012-01-01

    Full Text Available El maíz es un grano de consumo mundial para alimento tanto humano como animal. México se encuentra en el séptimo lugar de la producción, China y Estados Unidos son los principales consumidores. Zea mays pertenece a la familia de las gramíneas y tiene producción anual. La planta se puede ver afectada por plagas principalmente de diferentes especies de insectos. Puede también desarrollar enfermedad debida a hongos y virus, así como a bacterias patogénicas. Dentro de estas la causada por organismos del género Pantoea. P. stewartii se sabe que tiene genes como cps que codifican para la producción de exopolisacarido sterwatan controlada por un mecanismo de quórum sensing, y hrp para el sistema de secreción tipo III involucrados en la patogenicidad de la bacteria.

  3. Perfluorochemicals and Human Semen Quality: The LIFE Study

    Science.gov (United States)

    Chen, Zhen; Schisterman, Enrique F.; Kim, Sungduk; Sweeney, Anne M.; Sundaram, Rajeshwari; Lynch, Courtney D.; Gore-Langton, Robert E.; Barr, Dana Boyd

    2014-01-01

    Background: The relation between persistent environmental chemicals and semen quality is evolving, although limited data exist for men recruited from general populations. Objectives: We examined the relation between perfluorinated chemicals (PFCs) and semen quality among 501 male partners of couples planning pregnancy. Methods: Using population-based sampling strategies, we recruited 501 couples discontinuing contraception from two U.S. geographic regions from 2005 through 2009. Baseline interviews and anthropometric assessments were conducted, followed by blood collection for the quantification of seven serum PFCs (perfluorosulfonates, perfluorocarboxylates, and perfluorosulfonamides) using tandem mass spectrometry. Men collected a baseline semen sample and another approximately 1 month later. Semen samples were shipped with freezer packs, and analyses were performed on the day after collection. We used linear regression to estimate the difference in each semen parameter associated with a one unit increase in the natural log–transformed PFC concentration after adjusting for confounders and modeling repeated semen samples. Sensitivity analyses included optimal Box-Cox transformation of semen quality end points. Results: Six PFCs [2-(N-methyl-perfluorooctane sulfonamido) acetate (Me-PFOSA-AcOH), perfluorodecanoate (PFDeA), perfluorononanoate (PFNA), perfluorooctane sulfonamide (PFOSA), perfluorooctane sulfonate (PFOS), and perfluorooctanoic acid (PFOA)] were associated with 17 semen quality end points before Box-Cox transformation. PFOSA was associated with smaller sperm head area and perimeter, a lower percentage of DNA stainability, and a higher percentage of bicephalic and immature sperm. PFDeA, PFNA, PFOA, and PFOS were associated with a lower percentage of sperm with coiled tails. Conclusions: Select PFCs were associated with certain semen end points, with the most significant associations observed for PFOSA but with results in varying directions. Citation

  4. A successful new approach to honeybee semen cryopreservation.

    Science.gov (United States)

    Wegener, Jakob; May, Tanja; Kamp, Günter; Bienefeld, Kaspar

    2014-10-01

    Honeybee biodiversity is under massive threat, and improved methods for gamete cryopreservation could be a precious tool for both the in situ- and ex situ-conservation of subspecies and ecotypes. Recent cryoprotocols for drone semen have improved the viability and fertility of frozen-thawed semen by using increased diluent:semen-ratios, but there is still much room for progress. As semen cryopreserved after dilution often appeared hyperactive, we speculated that the disruption of sperm-sperm interactions during dilution and cryopreservation could reduce the fertile lifespan of the cells. We therefore developed protocols to reduce admixture, or abolish it altogether by dialyzing semen against a hypertonic solution of cryoprotectant. Additionally, we tested methods to reduce the cryoprotectant concentration after thawing. Insemination of queens with semen cryopreserved after dialysis yielded 49%, 59% and 79% female (= stemming from fertilized eggs) pupae in three separate experiments, and the numbers of sperm found in the spermathecae of the queens were significantly higher than those previously reported. Post-thaw dilution and reconcentration of semen for cryoprotectant removal reduced fertility, but sizeable proportions of female brood were still produced. Workers stemming from cryopreserved semen did not differ from bees stemming from untreated semen with regard to indicators of fluctuating asymmetry, but were slightly heavier. Cryopreservation after dialysis tended to increase the proportion of cells with DNA-nicks, as measured by the TUNEL-assay, but this increase appears small when compared to the baseline variations of this indicator. Overall, we conclude that cryoprotectant-addition through dialysis can improve the quality of cryopreserved drone semen. Testing of offspring for vitality and genetic integrity should continue.

  5. The effect of semen collection method and level of egg yolk on capability of dilution and storage of buck semen

    Directory of Open Access Journals (Sweden)

    N.N. Dhaher

    2013-12-01

    Full Text Available The study was conducted to evaluate the effect of semen collection method for reduction of the deleterious interaction between the enzymes of bulbourethral gland and egg yolk during the dilution and storage of buck semen by three different level of egg yolk. Ten bucks were used in this study; the bucks were divided into two groups (five bucks in each group. Semen samples were collected once a week for four weeks from the bucks in first group using an artificial vagina, and from the animals in second group using an electroejaculator. The collected semen samples were diluted with sodium citrate extender with three different level of egg yolk (5, 10 and 20%. Extend semen samples were stored at 5 °C for three days. Computer assisted sperm analysis and Sperm Class Analyzer® were used for evaluation of the buck semen samples. Sperm motility parameters were evaluated which includes; percentage of motile sperm, percentage of progressive motile sperm, the value of the linear velocity (VSL, the value of the average velocity (VAP, the value of the curvilinear velocity (VCL, and the amplitude of lateral movement of the head (ALH. Results showed that all sperm motility parameters under the different level of egg yolk in semen samples that collected by artificial vagina were significantly higher than those which collected by electroejaculator. The percentage of motile sperm and progressive motile sperm of samples that collected by artificial vagina were higher at 10% of egg yolk, while these motility parameters were higher at 5% of egg yolk for semen samples that collected by electroejaculator. The differences between the two methods of semen collection in VCL and ALH were clear and the values were higher in samples that collected using the artificial vagina. The values of VSL, VAP and VCL of semen samples that collected by artificial vagina were higher at the second day than first day of semen storage under 10% of egg yolk. In conclusion, there are effects

  6. Effect of semen extender and storage temperature on ram sperm motility over time

    Science.gov (United States)

    Storage of ram semen for long period of time depends on a number of factors, including type of extender and storage temperature. A study compared the effect of semen extender and storage temperature on motility of ram semen stored for 72 h. Semen collected via electroejaculator from 5 mature Katahd...

  7. Aspectos importantes del análisis de antibióticos

    OpenAIRE

    Montoya, Nelson

    2002-01-01

    Aspectos importantes del análisis de antibióticos Muchas drogas (antibióticos) y otros químicos, son comúnmente administrados durante el cultivo de organismos acuáticos, para prevenir o tratar infecciones u otras enfermedades bacterianas.

  8. Escova dental e dedeira na remoção da placa bacteriana dental em cães

    OpenAIRE

    Lima Tânia Berbert Ferreira; Eurides Duvaldo; Rezende Renata Junqueira; Milken Vanessa Martins Fayad; Silva Luiz Antonio Franco da; Fioravanti Maria Clorinda Soares

    2004-01-01

    A placa bacteriana é fator primário na formação de gengivite, cálculo dentário, halitose e doença periodontal. Objetivou-se avaliar a quantidade de placa bacteriana dental removida pela escova dental e dedeira. Foram utilizados 60 cães machos e fêmeas de diferentes raças, idade e peso, divididos em dois grupos. O índice Logan & Boyce foi utilizados para quantificar a placa bacteriana antes e após a escovação. Observou-se diferença estatística (p0,05) entre a utilização da escova dental e ...

  9. Environmental Exposure to Triclosan and Semen Quality.

    Science.gov (United States)

    Zhu, Wenting; Zhang, Hao; Tong, Chuanliang; Xie, Chong; Fan, Guohua; Zhao, Shasha; Yu, Xiaogang; Tian, Ying; Zhang, Jun

    2016-02-17

    Triclosan (2,4,4'-trichloro-2'-hydroxy-diphenyl ether, TCS) is widely used in personal care, household, veterinary and industrial products. It was considered as a potential male reproductive toxicant in previous in vitro and in vivo studies. However, evidence from human studies is scarce. Our study aims to investigate the relationship between TCS exposure and semen quality. We measured urinary TCS concentrations in 471 men recruited from a male reproductive health clinic. TCS was detected in 96.7% of urine samples, with a median concentration of 0.97 ng (mg·creatinine)(-1) (interquartile range, 0.41-2.95 ng (mg·creatinine)(-1)). A multiple linear regression analysis showed a negative association between natural logarithm (Ln) transformed TCS concentration (Ln-TCS) and Ln transformed number of forward moving sperms (adjusted coefficient β = -0.17; 95% confidence interval (CI) (-0.32, -0.02). Furthermore, among those with the lowest tertile of TCS level, Ln-TCS was negatively associated with the number of forward moving sperms (β = -0.35; 95% CI (-0.68, -0.03)), percentage of sperms with normal morphology (β = -1.64; 95% CI (-3.05, -0.23)), as well as number of normal morphological sperms, sperm concentration and count. Our findings suggest that the adverse effect of TCS on semen quality is modest at the environment-relevant dose in humans. Further studies are needed to confirm our findings.

  10. Resistencia bacteriana a los antibióticos: una crisis global

    OpenAIRE

    Alós Cortés, Juan Ignacio

    2015-01-01

    La introducción de los antibióticos en la práctica clínica supuso una de las intervenciones más importantes para el control de las enfermedades infecciosas. Los antibióticos han salvado millones de vidas, y además han supuesto una revolución en la medicina. Sin embargo, una amenaza creciente deteriora la eficacia de estos fármacos: la resistencia bacteriana a los antibióticos, que se define en este trabajo como la capacidad de una bacteria para sobrevivir en concentraciones de antibiótico que...

  11. Caracterização da microbiota bacteriana de Biomphalaria glabrata (Say, 1818)(Mollusca: Gastropoda)

    OpenAIRE

    Silva, Tatiana Maria da

    2012-01-01

    Devido a grande importância da microbiota no desenvolvimento de parasitas nos invertebrados, muitos estudos vêm sendo desenvolvidos para o melhor entendimento desta interação. Desta forma, a associação entre microrganismos e seus hospedeiros invertebrados, vetores de doenças, está se tornando cada vez mais evidente. Com base nisto, o objetivo do presente estudo foi caracterizar a microbiota bacteriana de caramujos da espécie Biomphalaria glabrata, principal hospedeiro interm...

  12. Desarrollo de Nanocompuestos de Celulosa Bacteriana para Aplicaciones Biomédicas

    OpenAIRE

    Grande Cruz, Cristian Jaime

    2014-01-01

    La celulosa bacteriana es un polisacárido obtenido a partir de microorganismos de la especie Gluconacetobacter cuando son cultivados en medios que contienen fuentes de carbono y nitrógeno. El material que se obtiene a partir de los procesos bioquímicos que ocurren dentro de la membrana celular de las bacterias se presenta físicamente como un gel que contiene agua y fibras de celulosa de alta pureza, cuyas dimensiones de sección se encuentran en el orden de los nanómetros. Debido a sus buenas ...

  13. Los bacteriófagos como una alternativa en el tratamiento de enfermedades infecciosas Bacterianas (Fagoterapia)

    OpenAIRE

    Nallelyt Segundo A.; Efrén Hernández B.; Oliver López V.; Oscar Torres A.

    2010-01-01

    Los bacteriófagos son virus que infectan y lisan bacterias. Se han aplicado en: Biología Molecular e Investigación Clínica, principalmente en el tratamiento de infecciones bacterianas (Fagoterapia). Los bacteriófagos se han utilizado contra Pseudomonas, Staphylococcus, Escherichia y Mycobacterium, resistentes a antibióticos. Existen compañías que comercializan fagos para terapia en humanos o animales además de otras aplicaciones como sanitizantes. Sin embargo, las farmacopeas mexicana y estad...

  14. Nanocompósitos baseados em celulose bacteriana para aplicações ópticas

    OpenAIRE

    Santos, Molíria Vieira dos [UNESP

    2012-01-01

    Neste trabalho membranas de biocelulose ou celulose bacteriana (CB), obtidas de meios de cultura da bactéria Gluconacetobacter xylinus, foram utilizadas como matriz para preparação de novos compósitos, a saber, celulose - colóides metálicos, celulose - sílica e celuloses - composto de európio. Nanopartículas (NP) esféricas de prata, ouro e prata-ouro, foram preparadas utilizando-se citrato de sódio e/ou pectina como agentes ao mesmo tempo redutores e estabilizantes. As propriedades ópticas de...

  15. Semen preservation and artificial insemination in domesticated South American camelids.

    Science.gov (United States)

    Bravo, P Walter; Alarcon, V; Baca, L; Cuba, Y; Ordoñez, C; Salinas, J; Tito, F

    2013-01-10

    Semen preservation and artificial insemination in South American camelids are reviewed giving emphasis to work done in Peru and by the authors. Reports on semen evaluation and the preservation process indicate that semen of alpacas and llamas can be manipulated by making it liquid first. Collagenase appears to be the best enzyme to eliminate viscosity. Tris buffer solution maintains a higher motility than egg-yolk citrate, phosphate buffered saline (PBS), Triladyl, and Merck-I extenders. Cooling of semen took 1h after collected, and equilibrated with 7% glycerol presented a better motility and spermatozoa survival at 1, 7, 15 and 30days after being slowly frozen in 0.25mL plastic straws. Trials of artificial insemination with freshly diluted semen and frozen-thawed semen are encouraging and needs to be tested extensively under field conditions. Recently, fertility rates varied from 3 to 67%. Semen preservation and most important, artificial insemination appear to be a reality, and could be used to improve the genetic quality of alpacas and llamas.

  16. Comparison of four diluents for the retriever dogs semen preservation

    Directory of Open Access Journals (Sweden)

    A. Wicaksono

    2009-03-01

    Full Text Available The quality of chilled semen depends on the composition of diluent. The choice of the buffer, anti-cold shock and nutrition sources can be the first decision in order to choose appropriate diluents. Nowadays a lot of diluent are used for canine semen preservation such as Tris buffer and Citrate buffer. This study was aimed to observe the differences of diluent for preserving Retriever dog spermatozoa. The semen sample collected from four Retriever dogs with three times repetition. The semen was evaluated macro-and microscopically. The semen with >70% sperm motility was divided into four tubes and diluted with diluter 1 (P1, diluter: P2, P3 and P4 (modified P3. The diluted semen was divided into two tubes and each sample was stored at room and 50C temperature. The viability of chilled semen was observed every 3 hours at room temperature and 12 hours at 50C. The result showed that P2 keep the sperm viability better than the other diluents. On 50C at 24 hours storage P2 showed the highest motile and live sperm percentage (46.25 ± 0.22%; 57.11 ± 0.25%. In room temperature at 6 hours P2 showed the highest motile and live sperm percentage (40.94 ± 0.20%; 52.65 ± 0.23%. It is concluded that P2 can keep the sperm viability by 84 hours of 50C and 21 hours at room temperature.

  17. Administração endovenosa de antibióticos e resistência bacteriana: responsabilidade da enfermagem

    OpenAIRE

    Heloisa Helena Karnas Hoefel; Liana Lautert

    2006-01-01

    O sucesso da terapêutica com antibióticos e o desenvolvimento da resistência bacteriana dependem de diversos fatores, sendo que os relacionados ao cuidado de enfermagem são o seu preparo e a sua administração. Objetivo: realizar uma revisão da literatura sobre falhas na administração de antibióticos analisando sua possível influência na resistência bacteriana. Delineamento: realizada uma pesquisa bibliográfica das fontes do LILACS e Medline. Métodos: realizada revisão bibliográfica sistematiz...

  18. Administração endovenosa de antibióticos e resistência bacteriana : responsabilidade da enfermagem

    OpenAIRE

    Hoefel, Heloisa Helena Karnas; Lautert, Liana

    2006-01-01

    O sucesso da terapêutica com antibióticos e o desenvolvimento da resistência bacteriana dependem de diversos fatores, sendo que os relacionados ao cuidado de enfermagem são o seu preparo e a sua administração. Objetivo: realizar uma revisão da literatura sobre falhas na administração de antibióticos analisando sua possível influência na resistência bacteriana. Delineamento: realizada uma pesquisa bibliográfica das fontes do LILACS e Medline. Métodos: realizada revisão bibliográfica sistematiz...

  19. Perfil etiológico das meningites bacterianas em crianças Etiological profile of bacterial meningitis in children

    OpenAIRE

    Mantese,Orlando C.; Jorge Hirano; Silva,Valéria M.; Irenize C. Santos; Elísio de Castro

    2002-01-01

    Objetivo: analisar o perfil etiológico e alguns aspectos epidemiológicos das crianças com meningite bacteriana, internadas em um hospital público universitário. Métodos: foram seguidas, prospectivamente, as crianças internadas com meningite bacteriana, diagnosticada segundo os critérios clínicos e laboratoriais habituais. Foram excluídos os casos de meningite pós-trauma, de meningite na vigência de derivação liquórica, ou de defeitos congênitos do tubo neural, e de meningite tuberculosa. Resu...

  20. Clinical Practice Guidelines for Bacterial Meningoencephalitis. Guía de práctica clínica para el tratamiento de la meningoencefalitis bacteriana.

    Directory of Open Access Journals (Sweden)

    Luciano Núñez Almoguea

    2009-03-01

    Full Text Available Clinical Practice Guidelines for Bacterial Meningoencephalitis. It has been defined as an acute inflammatory process caused by bacteria, often purulent, which involves the meninges, subarachnoid space around the brain, spinal cord and usually includes the ventricles. It is caused in the 80% of the patients by three bacteria: Haemophilus influenzae, Neisseria meningitides and Streptococcus pneumonia. Concepts, classification, diagnosis and treatment were reviewed. It includes assessment guidelines focused on the most important aspects to be accomplished.Guía de práctica clínica para el tratamiento de la meningoencefalitis bacteriana. Definida como un proceso inflamatorio agudo causado por bacterias, muchas veces purulento, que involucra a membranas meníngeas, espacio subaracnoideo alrededor del cerebro, médula espinal y suele incluir los ventrículos. Causada en cerca del 80 % por tres bacterias: Haemophilus influenzae, Neisseria meningitidis y Streptococcus pneumoniae. Se revisan los conceptos, clasificación, diagnóstico y tratamiento. Concluye con su guía de evaluación, enfocada en los aspectos más importantes a cumplir.

  1. Involuntary reduction in vigour of calves born from sexed semen.

    Science.gov (United States)

    Djedović, Radica; Bogdanović, Vladan; Stanojević, Dragan; Nemes, Zsolt; Gáspárdy, András; Cseh, Sándor

    2016-06-01

    The objective of this study was to compare the reproductive traits of heifers and the development characteristics of their calves following artificial insemination (AI) with sexed and non-sexed semen. The analysed characteristics included conception rate, gestation length, calf birth weight, calf vigour, stillbirth rate, and twinning rate. Data of 530 calves produced with sexed and 1,163 calves produced with non-sexed semen were analysed. The General Linear Model (GLM) was applied to assess the influence of semen type, farm, season of insemination, the calf's sex and the inseminating sire on gestation length and calf birth weight. With the exception of gestation length (P > 0.05), all other traits studied were significantly (P gestation length was 274.6 and 274.9 days, respectively. The mean calf birth weight was 37.47 kg for non-sexed and 36.75 kg for sexed semen. The stillbirth rate was 6.19% for conventional and 7.54% for sexed semen, while the twinning rate was 3.78% for conventional and 1.13% for sexed semen. The calves produced with non-sexed and sexed semen differed significantly in viability (P 0.05); however, artificial insemination with X-sorted sexed semen significantly altered the sex ratio of calves (85.1:14.9%, P < 0.01). The results obtained in this investigation are in agreement with the majority of studies which compared the fertility traits, sex ratio and calf characteristics depending on the application of artificial insemination with sexed or conventional semen.

  2. Efecto del cobre y del cadmio sobre la respuesta inmune innata del pez Colossoma macropomum

    Directory of Open Access Journals (Sweden)

    A. Vargas

    2012-01-01

    Full Text Available En este trabajo se evaluó la respuesta inmunológica celular inespecífica del pez Colossoma macropomum (Cuvier, 1818 expuesto a concentraciones subletales de cobre y cadmio, a través de la determinación de la viabilidad celular, la quimiotaxis, la fagocitosis y la muerte bacteriana en células inmunocompetentes extraídas del riñón cefálico, la sangre y el hígado. Los peces fueron expuestos por separado a 0,5 mg/L de cloruro de cobre y 1,0 mg/L de cloruro de cadmio durante 28 días; después de la exposición, se depuraron por el mismo período. La viabilidad celular se mantuvo entre 99-94%. La exposición al cadmio causó una reducción significativa en la respuesta quimiotáctica de granulocitos en los tejidos evaluados. Tanto los peces expuestos a cobre como los expuestos a cadmio presentaron una disminución significativa en la respuesta fagocítica de granulocitos. En sangre, el cadmio afectó la efectividad de los granulocitos de inducir muerte bacteriana; el cobre sin embargo, afectó la efectividad de inducir muerte bacteriana en los granulocitos provenientes del hígado. Los peces expuestos a cadmio presentaron un incremento significativo en los niveles de cortisol sérico y una disminución de esta hormona en los peces expuestos a cobre. Estos resultados demuestran que la exposición crónica a los metales cobre y cadmio produce una inhibición de la respuesta inmune inespecífica en el pez Colossoma macropomum.

  3. Evaluation of sperm chromatin structure in boar semen

    Directory of Open Access Journals (Sweden)

    Banaszewska Dorota

    2015-06-01

    Full Text Available This study was an attempt to evaluate sperm chromatin structure in the semen of insemination boars. Preparations of semen were stained with acridine orange, aniline blue, and chromomycin A3. Abnormal protamination occurred more frequently in young individuals whose sexual development was not yet complete, but may also be an individual trait. This possibility is important to factor into the decision regarding further exploitation of insemination boars. Thus a precise assessment of abnormalities in the protamination process would seem to be expedient as a tool supplementing morphological and molecular evaluation of semen. Disruptions in nucleoprotein structure can be treated as indicators of the biological value of sperm cells.

  4. MicroHLPC determination of amygdalin in Semen pruni armeniacae and Semen pruni persicae.

    Science.gov (United States)

    He, L Y; Li, B M

    1988-11-01

    The application of micro HPLC to the determination of amygdalin in Semen pruni armeniacae and Semen pruni persicae is described. Amygdalin is separated at ambient temperature on a reversed phase column of U-Finepak SIL C18(150 x 0.5 mm) with methanol + water (25:75 v/v) as the mobile phase at a flow rate of 10 microL/min. The results are calculated by the internal standard method. The linear range is 1-7 micrograms. The CV and recovery of pure amygdalin are 1.47% (n = 10) and 98.13%, respectively. The results of analysis are lower than those obtained by TLC, but microHPLC is much simpler, faster, and more sensitive and reproducible than TLC.

  5. Bacterial communities in semen from men of infertile couples: metagenomic sequencing reveals relationships of seminal microbiota to semen quality.

    Directory of Open Access Journals (Sweden)

    Shun-Long Weng

    Full Text Available Some previous studies have identified bacteria in semen as being a potential factor in male infertility. However, only few types of bacteria were taken into consideration while using PCR-based or culturing methods. Here we present an analysis approach using next-generation sequencing technology and bioinformatics analysis to investigate the associations between bacterial communities and semen quality. Ninety-six semen samples collected were examined for bacterial communities, measuring seven clinical criteria for semen quality (semen volume, sperm concentration, motility, Kruger's strict morphology, antisperm antibody (IgA, Atypical, and leukocytes. Computer-assisted semen analysis (CASA was also performed. Results showed that the most abundant genera among all samples were Lactobacillus (19.9%, Pseudomonas (9.85%, Prevotella (8.51% and Gardnerella (4.21%. The proportion of Lactobacillus and Gardnerella was significantly higher in the normal samples, while that of Prevotella was significantly higher in the low quality samples. Unsupervised clustering analysis demonstrated that the seminal bacterial communities were clustered into three main groups: Lactobacillus, Pseudomonas, and Prevotella predominant group. Remarkably, most normal samples (80.6% were clustered in Lactobacillus predominant group. The analysis results showed seminal bacteria community types were highly associated with semen health. Lactobacillus might not only be a potential probiotic for semen quality maintenance, but also might be helpful in countering the negative influence of Prevotella and Pseudomonas. In this study, we investigated whole seminal bacterial communities and provided the most comprehensive analysis of the association between bacterial community and semen quality. The study significantly contributes to the current understanding of the etiology of male fertility.

  6. Effects of sperm concentration at semen collection and storage period of frozen semen on dairy cow conception.

    Science.gov (United States)

    Haugan, T; Gröhn, Y T; Kommisrud, E; Ropstad, E; Reksen, O

    2007-01-01

    The present study was based on data obtained from artificial inseminations (AIs) performed with cryopreserved semen from elite bulls used in the Norwegian breeding program. Semen was diluted to standardize the number of spermatozoa to 18 million per AI dose. The aim of the study was to investigate whether the net sperm concentration at semen collection and the storage period in liquid nitrogen have any effect on probability of conception in dairy cattle. We demonstrated that the natural range of sperm concentration at semen collection within some of the bulls was associated with the probability of conception. However, no primary trend among bulls was found on the effect of sperm concentration at semen collection. This appears to be due to differences among bulls in their response to the dilution ratio of seminal plasma to extender. The effect of storage time was investigated in semen that had been stored between 1000 days and 2400 days in AI straws in liquid nitrogen at the AI center. Our findings showed that use of semen with the longest storage period, i.e. 1951-2400 days, resulted in a more than one percentage point lower probability of conception than semen with a shorter storage period. In conclusion, the net sperm concentration at semen collection, which affects the dilution ratio of seminal plasma to extender, should be considered individually among bulls to achieve optimal reproductive performance. Furthermore, this study gives support to the idea that a measurable degree of damage to the spermatozoa could occur during the preservation time in liquid nitrogen.

  7. Validación de la prueba de Papanicolaou en el diagnóstico de vaginosis bacteriana. Antioquia, Colombia

    Directory of Open Access Journals (Sweden)

    María Elena Vásquez Peña

    2002-01-01

    Full Text Available A descriptive cross sectional study was carry out in 423 women in seven rural communities of the municipality of Amagá, Antioquia. One of the objectives of this study was to evaluate the sensibility and specificity and predictive values of the test of Papanicolaou in relation to the Gram exam, for the diagnosis of Bacterial Vaginosis (BV. In the 423 studied women the results showed a prevalence of BV for the bacteriological exam Gram of 20.8 % (88 cases and for Papanicolaou of 21% (89 cases. The sensitivity was 87.5%, the specificity was 96.4%. The value predictive positive was 86,5% and the value predictive negative was 96.7%. The high sensitivity, specificity and predictive values of the Papanicolaou test reported in this study make it a good screening method, since it is an exam that is carried out commonly and no additional testa are required. Se realizó un estudio descriptivo de corte en 423 mujeres de la población rural del municipio de Amagá, Antioquia, cuyas edades estaban comprendidas entre los 15 y 65 años, a quienes se les tomó muestras cérvicovaginales para estudio citológico de Papanicolau y coloración de Gram. El objetivo de este estudio fue evaluar la sensibilidad, especificidad y valores predictivos de la prueba de Papanicolaou frente al Gram, para el diagnóstico de vaginosis bacteriana (vb. La prevalencia de vb por el Gram, en las 423 mujeres estudiadas, fue de 20.8% (88 casos y la prevalencia de vb por Papanicolaou, fue del 21 % (89 casos. La sensibilidad fue 87.5%, la especificidad 96.4%, valor predictivo positivo (VPP 86,5% y valor predictivo negativo (VPN 96.7%. La alta sensibilidad, especificidad y valores predictivos de la prueba de Papanicolaou, reportados en este estudio hacen de ésta un buen método de tamizaje, dado que es un examen que se realiza de rutina y no se requiere una prueba adicional para hacer el diagnóstico; lo que a su vez permite disminuir costos porque se omite el estudio de la flora vaginal

  8. A orbifloxacina no tratamento das cistites bacterianas em gatos domésticos

    Directory of Open Access Journals (Sweden)

    Reche Junior Archivaldo

    2005-01-01

    Full Text Available O presente estudo teve como objetivos, avaliar o uso da orbifloxacina nas cistites bacterianas de felinos, e seus possíveis efeitos colaterais, mormente a nefro e/ou hepatotoxicidade. Para tanto, foram colhidas amostras de urina de 53 gatos com históricos variados, mas com predisposição à infecção urinária. A cistite bacteriana foi comprovada, através de exame microbiológico, em 13 pacientes. No entanto, apenas 10 apresentaram infecção por agentes sensíveis a orbifloxacina. Esses 10 pacientes foram então submetidos ao tratamento com orbifloxacina na dose de 2,5mg kg-1 a cada 24 horas por 10 dias. Não houve crescimento bacteriano nas amostras de urina submetidas à cultura, 72 horas após a interrupção do tratamento. Não foram observados sinais de nefro e/ou hepatotoxicidade nesses pacientes, uma vez que os níveis séricos de alanina aminotransferase (ALT, aspartato aminotransferase (AST, fosfatase alcalina (FA, albumina, uréia e creatinina não tiveram variação significativa após o uso do fármaco. Portanto, os resultados apresentados sugerem que a orbifloxacina pode ser usada como agente antimicrobiano nas infecções urinárias de felinos.

  9. History of commercializing sexed semen for cattle.

    Science.gov (United States)

    Garner, D L; Seidel, G E

    2008-04-15

    Although the basic principles controlling the sex of mammalian offspring have been known for a relatively long time, recent application of certain modern cellular methodologies has led to development of a flow cytometric system capable of differentiating and separating living X- and Y-chromosome-bearing sperm in amounts suitable for AI and therefore, commercialization of this sexing technology. After a very long history of unsuccessful attempts to differentiate between mammalian sperm that produce males from those that produce females, a breakthrough came in 1981 when it was demonstrated that precise DNA content could be measured. Although these initial measurements of DNA content killed the sperm in the process, they led to the ultimate development of a sperm sorting system that was capable, not only of differentiating between live X- and Y-sperm, but of sorting them into relatively pure X- and Y-sperm populations without obvious cellular damage. Initial efforts to predetermine the sex of mammalian offspring in 1989 required surgical insemination, but later enhancements provided sex-sorted sperm in quantities suitable for use with IVF. Subsequent advances in flow sorting provided minimal numbers of sperm sufficient for use in AI. It was not until the flow cytometric sorting system was improved greatly and successful cryopreservation of sex-sorted bull sperm was developed that efficacious approaches to commercialization of sexed semen could be implemented worldwide in cattle. A number of companies now offer sex-sorted bovine sperm. Innovative approaches by a diverse group of scientists along with advances in computer science, biophysics, cell biology, instrumentation, and applied reproductive physiology provided the basis for commercializing sexed semen in cattle.

  10. Environmental Exposure to Triclosan and Semen Quality

    Directory of Open Access Journals (Sweden)

    Wenting Zhu

    2016-02-01

    Full Text Available Triclosan (2,4,4′-trichloro-2′-hydroxy-diphenyl ether, TCS is widely used in personal care, household, veterinary and industrial products. It was considered as a potential male reproductive toxicant in previous in vitro and in vivo studies. However, evidence from human studies is scarce. Our study aims to investigate the relationship between TCS exposure and semen quality. We measured urinary TCS concentrations in 471 men recruited from a male reproductive health clinic. TCS was detected in 96.7% of urine samples, with a median concentration of 0.97 ng (mg·creatinine−1 (interquartile range, 0.41–2.95 ng (mg·creatinine−1. A multiple linear regression analysis showed a negative association between natural logarithm (Ln transformed TCS concentration (Ln-TCS and Ln transformed number of forward moving sperms (adjusted coefficient β = −0.17; 95% confidence interval (CI (−0.32, −0.02. Furthermore, among those with the lowest tertile of TCS level, Ln-TCS was negatively associated with the number of forward moving sperms (β = −0.35; 95% CI (−0.68, −0.03, percentage of sperms with normal morphology (β = −1.64; 95% CI (−3.05, −0.23, as well as number of normal morphological sperms, sperm concentration and count. Our findings suggest that the adverse effect of TCS on semen quality is modest at the environment-relevant dose in humans. Further studies are needed to confirm our findings.

  11. SPONTANEOUS POTENTIAL INVESTIGATIONS IN SEMENIC MOUNTAINS

    Directory of Open Access Journals (Sweden)

    P. URDEA

    2014-11-01

    Full Text Available Spontaneous Potential Investigations in Semenic Mountains. The use of geophysical methods such as that of Spontaneous Potential (SP to investigate areas where the geomorphological processes occur, has the role to identify less visible processes as for example subcutaneous erosion or piping, subsoil water drainage and finding specific spatial differences of these processes. Comparative study of these sites allows correlation between geomorphological factors, soil and climate, but also to observe the evolution of subsurface erosion or underground water infiltration over time. During this investigation a series of mesh grids have been made in areas with different characteristics (lithology, pedology, slope, exposition, etc. at different time periods in order to spot and analyse the change in data in the chosen sites, various conditions given. Values expressed in millivolts (mV obtained by the Spontaneous Potential method have been put into an algorithm for interpolation looking to yield a pattern of values of what is happening in the soil during that period of time. Thus, in the autumn, the investigation site at the nivation niche Baia Vulturilor, returned values of between -22.6 mV and 65.6 mV, while in spring in the same site, values were within the range of -14.4 mV / 30.1 mV. On the other hand, on the site of the cryopediment under the Semenic peak, in the spring, return values ranged from -40.4 mV and -1.1 mV. A particular case is that of the glacis near Piatra Goznei peak; in this area anthropogenic electricity influences on soil can be found. Based on some models a trend of water movement in the soil could be established, this depending heavily on the amount of precipitation infiltration, local lithology, depth of soil and their structure, and evapotranspiration process. Water movement in the soil may be a correlation with sediment movement in soil horizons and instability manifested on the slopes.

  12. Research Development on Cryopreservation Technique to Preserve Avian Semen

    Directory of Open Access Journals (Sweden)

    Tatan Kostaman

    2011-09-01

    Full Text Available Cryopreservation technique could be used to preserve animal cell, plant or other genetic materials (included semen in frozen. In this case, the cryopreservation technique is a storage technique that carries out at very low temperature in liquid nitrogen at -196oC. At this temperature, semen does not experience the process of metabolism but still has the ability to live on when used later. Semen that is preserved by cryopreservation technique has unlimited shelf life. This method is more efficient in terms of cost, time, space, and labour than other methods. Cryopreservation techniques can be divided into conventional technique (controlled slow freezing and rapid freezing technique. Besides cryopreservation of semen, other genetic material from avian that can be cryopreservesed is Primodial Germ Cells (PGC. Balitnak has succesfully isolated the PGC of some Indonesian native chickens. The success of cryopreservation is indicated by not only the high rate of survival, but also the fertility after cryopreservation.

  13. Relación entre la estructura de la comunidad bacteriana y la supresión al ahogamiento causado por Rhizoctonia solani en sustratos para plántulas de tomate

    Directory of Open Access Journals (Sweden)

    O. Robledo

    2010-01-01

    Full Text Available En este trabajo el objetivo fue estudiar la relación entre la supresión del ahogamiento causado por Rhizoctonia solani y la estructura de la comunidad bacteriana en sustratos orgánicos (con base en vermicomposta y composta, para el crecimiento de plántulas de tomate. Dicha estructura comunitaria se determinó mediante T-RFLP (Terminal Restriction Fragment Length Polymorphisms del gen ARNr 16S, con la enzima Hin6I. Análisis de componentes principales (PCA a partir de las abundancias relativas de los T-RFs (Fragmentos de Restricción Terminales predominantes, mostraron que los sustratos con tendencia supresiva al patógeno poseen comunidades bacterianas similares, indicando la existencia de una relación entre la estructura de la comunidad y la supresividad. Los T-RFs predominantes se relacionaron con secuencias obtenidas de una librería genómica del gen ribosomal ARNr 16S de un sustrato con tendencia supresiva. Dicho análisis de PCA reveló que entre los TRFs que contribuyen a la diferenciación de los sustratos con tendencias supresivas, se encuentran tres (64, 205 y 211 que corresponden a secuencias 16S de los siguientes géneros: Burkholderia, Duganella, Telluria, Janthinobacterium, Pseudmomonas, Stenotrophomonas y Xanthomonas. Tales géneros presentan especies con capacidad biocontroladora específica sobre el patógeno, por lo que estos resultados sugieren que los T-RFs (Hin6I 64, 205, 211 y otros, podrían ser utilizados como indicadores a priori de la capacidad supresiva de un sustrato al ahogamiento causado por Rhizoctonia solani.

  14. In vitro fertility assessment of Kundhi buffalo bull semen

    Directory of Open Access Journals (Sweden)

    Hamzo Khan Kunbhar,

    2011-02-01

    Full Text Available The study was conducted on in-vitro fertility assessment of frozen thawed semen collected from Kundhi buffalo bull maintained at Department of Animal Reproduction, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam. Before freezing of semen, each ejaculate was assessed for volume, sperm concentration, mass activity and moss motility percentage. Twenty semen samples having motility 60% or above were frozen for post-thaw assessment. Frozen thawed semen was incubated at 250C for 5 hours and examined for progressive linear motility and live dead sperm count. The mean volume, mass activity, moss motility percentage sperm concentrations and pH of the semen were found to be 2.79±0.217 ml, 2.85±0.111, 71.75±2.621, 11.35±1.255 millions/ml and 5.8185±0.092 respectively of fresh semen. No significant difference was found between the parameters except pH, which was significantly different between the bulls. The mean sperm motility percentage and live dead sperm count % of Kundhi buffalo bull semen was found to be 20.46±1.62 and 6.9± 0.2% for frozen semen. A significant (P< 0.05 difference was found between the bulls for post-thaw motility percentage. It was found that at 01 hour incubation, 43.25±2.95% of sperms were motile having 11.78±0.28 % dead sperm count. It was gradually decline from 0 to 5 hours incubation, After 5 hours, all sperms were found dead. It is concluded that sperms maintaining long term motility and having less live dead sperms count were considered suitable for artificial insemination.

  15. Artificial insemination and cryopreservation of semen from nondomestic birds

    Science.gov (United States)

    Gee, G.F.; Bakst, M.R.; Wishart, G.J.

    1995-01-01

    Studies of Al and cryopreservation of semen from nondomestic birds began because of the increased emphasis on conservation of avian species threatened with extinction. Over the years, aviculturists have developed techniques for Al and cryopreservation of semen obtained from a variety of birds ranging from passerines to Andean condors. Generally, for each new species, we develop a practical semen collection technique and then evaluate the semen. A commercial semen extender (Beltsville Poultry Semen Extender) is modified and used to dilute the semen and provide support for the sperm during the freezing process (the pH and osmolality of the extender is adjusted to reflect the pH and osmolality of the semen being frozen). We find that the freezing schedule developed by Sexton (1977), which utilizes dimethylsulfoxide (DMS0) as cryoprotectant, works well for many species. We cool the sample sequentially in an ethanol bath, in liquid nitrogen vapor, and lastly in liquid nitrogen. Although we have experimented with a variety of freezing protocols, we prefer a 15-min equilibration period in DMSO at 5 C. We begin the freezing process by cooling at -1 C/min from 5 to -20 C in the ethanol bath. The samples are transferred into a vapor tank at a location just above liquid nitrogen and frozen at -50 C/min to -80 C. To complete the freezing process, the samples are plunged into the liquid nitrogen in the bottom of the vapor tank. The samples remain in liquid nitrogen until they are thawed just before insemination. If necessary, the freezing equipment can be transported in a van to remote locations.

  16. Semen Displacement as a Sperm Competition Strategy in Humans

    Directory of Open Access Journals (Sweden)

    Gordon G. Gallup

    2004-01-01

    Full Text Available We examine some of the implications of the possibility that the human penis may have evolved to compete with sperm from other males by displacing rival semen from the cervical end of the vagina prior to ejaculation. The semen displacement hypothesis integrates considerable information about genital morphology and human reproductive behavior, and can be used to generate a number of interesting predictions.

  17. Efektifitas Penambahan Flour Pada Bahan Restorasi Semen Ionomer Kaca

    OpenAIRE

    Dewi Sri Kesumawati

    2008-01-01

    Semen ionomer kaca adalah bahan tumpatan yang dalam penggunaannya dapat mempertahankan atau menggantikan jaringan gigi sebanyak mungkin, membantu terjadinya proses remineralisasi, dan mempunyai estetik yang baik. Bahan restorasi yang biasa digunakan dan dapat melepaskan fluor adalah semen ionomer kaca. Bahan ini sebagai perekat yang mengikat struktur gigi dan melepas fluor sebanyak yang dibutuhkan gigi agar dapat mencegah terjadinya karies. Dentin merupakan lapisan dibawah email, sehingga...

  18. Study of semen parameters in male partners among infertile couples

    Directory of Open Access Journals (Sweden)

    Sheela N. Kulkarni

    2015-08-01

    Full Text Available Background: The Semen analysis provides valuable information about the etiology and fertility potential of an infertile male. The study was conducted to determine the abnormalities in semen parameters of male partners of infertile couples and to find out contribution of male factors. Methods: The descriptive study with cross sectional design was conducted in the department of Pathology at MIMSR medical college, Latur, Maharashtra, India, between January 2013 to December 2014. A total of 220 cases were analyzed during this period. Semen analysis was performed according to the methods and the standards defined by World Health Organisation (WHO 5th edition 2010. Results: Out of 220 male partners of infertile couples 96 (43.6% men had abnormal semen parameters. The male factor was responsible in 43.6% of cases. Asthenozoospermia constitutes maximum of 19.9%, followed by Oligozoospermia in 18.6%, Azoospermia in 10.9%, Oligoasthenoteratozoospermia in 7.3% and Oligoasthenozoospermia in 6.8% cases. Leucocytospermia was detected in 15.5% cases. Conclusions: Abnormal semen quality remains a significant contribution to overall infertility. Asthenozoospermia is the most common semen abnormality seen. [Int J Reprod Contracept Obstet Gynecol 2015; 4(4.000: 1016-1019

  19. The utility of nanowater for ram semen cryopreservation.

    Science.gov (United States)

    Murawski, Maciej; Schwarz, Tomasz; Grygier, Joanna; Patkowski, Krzysztof; Oszczęda, Zdzisław; Jelkin, Igor; Kosiek, Anna; Gruszecki, Tomasz M; Szymanowska, Anna; Skrzypek, Tomasz; Zieba, Dorota A; Bartlewski, Pawel M

    2015-05-01

    Nanowater (NW; water declusterized in the low-temperature plasma reactor) has specific physicochemical properties that could increase semen viability after freezing and hence fertility after artificial insemination (AI) procedures. The main goal of this study was to evaluate ram semen quality after freezing in the media containing NW. Ejaculates from 10 rams were divided into two equal parts, diluted in a commercially available semen extender (Triladyl®; MiniTüb GmbH, Tiefenbach, Germany) prepared with deionized water (DW) or NW, and then frozen in liquid nitrogen. Semen samples were examined for sperm motility and morphology using the sperm class analyzer system and light microscopy. Cryo-scanning electron microscopy (cryo-SEM) was employed to determine the size of extracellular water crystals in frozen semen samples. Survival time at room temperature, aspartate aminotransferase (AspAT) and alkaline phosphatase (ALP) concentrations post-thawing as well as conception/lambing rates after laparoscopic intrauterine AI of 120 ewes were also determined. There were no significant differences between DW and NW groups in sperm progressive motility (26.4 ± 12.2 and 30.8 ± 12.4%) or survival time (266.6 ± 61.3 and 270.9 ± 76.7 min) after thawing and no differences in the percentages of spermatozoa with various morphological defects before or after freezing. There were, however, differences (P ram semen and lamb productivity of inseminated ewes.

  20. Diversidade e estrutura da comunidade bacteriana associada às armadilhas da planta carnívora Utricularia gibba (Lentibulariaceae) e ao ambiente aquático.

    OpenAIRE

    Almir José Ferreira

    2011-01-01

    A diversidade microbiana em ambientes aquáticos e sua associação com plantas carnívoras ainda é pouco estudada. Assim, a comunidade bacteriana da planta carnívora Utricularia gibba e do seu meio aquático foi avaliada por meio do seqüenciamento em larga escala (454 Roche) de uma biblioteca do gene 16S rRNA. Os resultados indicaram que a comunidade bacteriana na água é significativamente diferente da comunidade dos utrículos. Além disso, a comunidade bacteriana da água é composta principalmente...

  1. Identifying factors affecting age at first semen freezing and age at first semen use in Sahiwal bulls

    Directory of Open Access Journals (Sweden)

    B. C. Naha

    2015-07-01

    Full Text Available Aim: The objective of the study was to evaluate the effects of non-genetic factors on reproduction traits viz. age at first semen freezing and age at first semen use of breeding bulls in Sahiwal bulls by fitting least-squares analysis. Materials and Methods: The information on reproduction traits of 43 Sahiwal breeding bulls belonging to 8 sets of Sahiwal breeding program at Indian Council of Agricultural Research-National Dairy Research Institute (ICAR-NDRI, Karnal (Haryana, India during 27 years (1987-2013 were analyzed using fixed linear model. The information was collected from AI records, reproduction sheets, and bull AI register maintained at different sections of Institute viz. record room of Dairy Cattle Breeding Division (DCB, Cattle Yard, Artificial Breeding Research Centre, ICAR-NDRI, Karnal. Results: The average age at first semen freezing and age at first semen use of Sahiwal breeding bulls was estimated as 3.17±0.01 years and 5.35±0.01 years, with the coefficient of variation 18.93% and 20%, respectively. The overall least squares mean for age at first semen freezing and age at first semen use was estimated as 3.14±0.09 years and 5.25±0.02 years, respectively, in Sahiwal breeding bulls. Period of freezing/use had significant effects on reproductive traits (p<0.01. Season had no significant effect on any of the traits considered in this study. Conclusion: It can be concluded that management inputs such as nutrition, breeding, and optimum environment should be taken care of to optimize age at first semen freezing and age at first semen use for better utilization of superior germplasm.

  2. Identifying factors affecting age at first semen freezing and age at first semen use in Sahiwal bulls

    OpenAIRE

    Naha, B. C.; Chakravarty, A. K.; Mir, M. A.; V. Jamuna; Singh, A. P.; Maher, D

    2015-01-01

    Aim: The objective of the study was to evaluate the effects of non-genetic factors on reproduction traits viz. age at first semen freezing and age at first semen use of breeding bulls in Sahiwal bulls by fitting least-squares analysis. Materials and Methods: The information on reproduction traits of 43 Sahiwal breeding bulls belonging to 8 sets of Sahiwal breeding program at Indian Council of Agricultural Research-National Dairy Research Institute (ICAR-NDRI), Karnal (Haryana), India durin...

  3. Estudo da incidência de vaginose bacteriana em parturientes pré-termo ou gestantes com amniorrexe prematura

    Directory of Open Access Journals (Sweden)

    Raquel G. A. Torres

    2008-01-01

    Full Text Available Objetivos: relacionar a incidência de vaginose bacteriana entre parturientes com diagnóstico de trabalho de parto prematuro ou gestantes com amniorexe prematura em Sorocaba/SP. Metodologia: estudamos, de setembro/2003 a junho/2004, 30 pacientes internadas com diagnóstico clínico de trabalho de parto prematuro e/ou amniorexe prematura com idade gestacional inferior a 37 semanas. Como grupo controle tivemos 30 gestantes internadas para tratamento de outras intercorrências clínicas e/ou obstétricas, pareadas com o grupo de estudo pela idade cronológica e idade gestacional. A vaginose bacteriana foi diagnosticada pelo pH vaginal superior a 4.5, liberação de odor de “peixe podre” ao adicionar KOH (10% ao conteúdo vaginal, presença de “clue-cell” nesse material, e a caracterização de esfregaço sugestivo de vaginose bacteriana pelo método de Gram. Resultados: a vaginose bacteriana foi encontrada em 10.0% das pacientes do grupo de estudo e em 13.3% das pacientes do grupo controle. Encontramos também a presença de processos infecciosos vaginais, especialmente causados por leveduras, ambos achados não apresentaram significância estatística. Conclusão: a vaginose bacteriana não se associou de maneira significante com a ocorrência de trabalho de parto prematuro e/ou amniorexe prematura. Trabalho subsidiado por bolsa PIBIC-CNPq.

  4. Human semen assays for workplace monitoring. [Monitoring of hazardous materials by determining effects on semen of personnel

    Energy Technology Data Exchange (ETDEWEB)

    Wyrobek, A.J.; Gledhill, B.L.

    1978-11-07

    Decades of human semen studies have yielded compelling evidence that sperm can be used to access reproductive potential and diagnose pathology. With these studies as background, the small number of detailed semen studies of men exposed to physical and chemical agents point with optimism to the application of human semen assays as efficient, effective means to monitor for reproductive hazards in the workplace. Sperm are the most accessible of human gonadal tissue and provide a means of monitoring exposure induced changes in the human testes, changes which may result in infertility and increased frequencies of genetically abnormal gametes. The focus on semen has precipitated the development of new sperm bioassays which use older conventional andrological methods (i.e., sperm counts, motility, and morphology) as well as recently developed high speed flow and scanning methods for automated cytological analyses. The status of these sperm assays for workplace surveillance is reviewed, procedures are suggested with examples of use, and their effectiveness is evaluated. The available mouse models of induced semen changes are briefly described and the importance of these models for evaluating the genetic implications of findings in human semen is discussed.

  5. Association of Blood and Semen Lead and Zinc Level with Semen Parameter in the Male Partner of Infertile Couple.

    Science.gov (United States)

    Fatima, P; Hossain, M M; Rahman, D; Rahman, M W; Mugni, C R; Sumon, G M; Hossain, H B; Hossain, H N

    2015-07-01

    This cross sectional study was carried out in Center for Assisted Reproduction, Dhaka, and in the Department of Biochemistry, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh from July 2012 to December 2012. The study population was 71 consecutive male partners of infertile couple suffering from at least one year of infertility. Lead and Zinc level was measured in blood and semen in the male partner of infertile couple and compared with semen parameters. Serum zinc at different values did not show any statistically significant change in semen volume, total count of sperm and total motility of sperm. At serum zinc level 80-sperm (54.00 ± 46.67 million/ml) but was not statistically significant. Rapid linear motility and normal sperm morphology was also highest at values 80-zinc level > 90 μg/dl semen lead level was significantly higher (120.73 ± 58.02 μg/dl) and showed statistically significant decrease in rapid linear motility and normal sperm morphology. Total count of sperm was lowest at blood zinc level of 70-Sperm morphology also showed statistically significant improvement at Serum zinc values of 80-zinc level of values 80-zinc levels higher as well as lower than values 80-< 90 μg/dl was associated with increased semen lead values and with negative impact on semen parameters.

  6. Comparative Examination of Capercaillie (Tetrao urogallus L. Behaviour Responses and Semen Quality to Two Methods of Semen Collection.

    Directory of Open Access Journals (Sweden)

    Ewa Teresa Łukaszewicz

    Full Text Available Artificial insemination (AI is very helpful in solving the reproductive and biodiversity problems observed in small, closed avian populations. The successful production of fertilized eggs using AI is dependent on the collection of good quality semen. Two methods of male sexual stimulation and semen collection from captive kept capercaillie (Tetrao urogallus L., one of the most seriously endangered grouse species in Europe, are compared in this study. Ejaculates were obtained either with the use of a dummy female or by the dorso-abdominal massage method. Differences in the individual responses of the males to the two methods of semen collection as well as in their semen quality were noted. Only sperm concentration (432.4 x 10(6 mL(-1 with dummy female and 614.5 x 10(6 mL(-1 for massage method was significantly affected by capercaillie stimulation method. Sperm motility and morphology were not affected (P ≥ 0.05. Thus, for semen collection from captive kept capercaillie both methods can be used successfully. The dummy female can be an alternative to dorso-abdominal massage method, commonly used for semen collection from domesticated bird species.

  7. Placa bacteriana dentária em cães Dental bacterial plaque in dogs

    Directory of Open Access Journals (Sweden)

    Duvaldo Eurides

    1996-12-01

    Full Text Available Com objetivo de avaliar a incidência de placa bacteriana dentária, foram utilizados 30 cães, sem raça definida, distribuídos em três grupos iguais, de acordo com a faixa etária, sendo o grupo I de 0 a 2 anos, o grupo II de 3 a 5 anos e o grupo III acima de 6 anos. Os animais foram submetidos a anestesia geral e sobre os dentes foi aplicado cloreto de metilrosanilina, para evidenciar acúmulos bacterianos. As placas encontravam-se distribuídas em 80,99% no grupo I, 71,45% no grupo II e 83,96% no grupo III. Os grupos dentários foram considerados separadamente, apresentando índices de 64,84% nos dentes incisivos, 84,80% nos caninos, 87,23% nos pré-molares e 78,34% nos molares. Nos três grupos de cães observou-se índices semelhantes de placa bacteriana nos diferentes grupos dentários. Os animais do grupo II e o grupo de dentes incisivos apresentaram menores índices de placa bacteriana.To avaliate the incidence of bacterial plaque 30 mogreal dogs were utilizated, distributed among three equal groups. According to age, group I was composed of 0 to 2 years-old dogs, group II was 3 to 5 years-old, and group III over 6 years-old. The animals underwent a general anesthesia and metilrosanilin cloret was applied over the tooth, to evidence the bacterial mass. The plaques were distributed to 80.99% in group I, 71.42% in group II and 83.96% in group III. The dental groups were considerated apart, showing index of 64.84% on the incisar tooth, 84.80% on the canine tooth, 87.23% on the premolar tooth, and 78.34% on the molar tooth. At the dogs groups, similar Índex of bacterial plaque were observed on the diffèrent dental groups. The dogs from group II and the incisive tooth group showed minar bacterial plaque index.

  8. SEMEN QUALITY ASSESSMENT OF NEW ZEALAND WHITE RABBIT BUCKS

    Directory of Open Access Journals (Sweden)

    Martyna Błaszczyk

    2013-02-01

    Full Text Available Rabbits have been extensively used as a model for large animals and humans. All the reproduction techniques employed with farm animals can be performed with the low-cost rabbit model, and certain placental membrane characteristics make them especially relevant for studies of human teratology. The purpose of this study was to assess semen quality of New Zealand White rabbits. The material represents semen samples collected from adult rabbits (n=30. The semen was obtained by means of artificial vagina. All samples were analyzed using CASA Sperm VisionTM system. To assessed spermatozoa morphology (the length and the width of head and tail; presence of abnormal spermatozoa we used QuickPhoto Micro system. Received data were statistically analyzed. Our research showed decrease of semen parameters value after one hour storage in 37°C. Correlation analysis showed negative correlation between presence of spermatozoa with separated flagellum and CASA parameters value e.g. motility, progressive motility, DAP, DCL, DSL, VAP, VCL, VSL, ALH and BCF. From among 3000 analyzed spermatozoa 14.2% posed abnormal forms. We observed negative influence of semen storage on its quality. Also negative correlations between all types of tail defect and motility of spermatozoa were detectedRabbits have been extensively used as a model for large animals and humans. All the reproduction techniques employed with farm animals can be performed with the low-cost rabbit model, and certain placental membrane characteristics make them especially relevant for studies of human teratology. The purpose of this study was to assess semen quality of New Zealand White rabbits. The material represents semen samples collected from adult rabbits (n=30. The semen was obtained by means of artificial vagina. All samples were analyzed using CASA Sperm VisionTM system. To assessed spermatozoa morphology (the length and the width of head and tail; presence of abnormal spermatozoa we used Quick

  9. Evaluación de la eficacia de dos prescripciones de cepillos dentales en la remoción de placa bacteriana en pacientes ortodóncicos Evaluation of two toothbrushes prescriptions on the removal of plaque in patients with fixed orthodontic appliances

    OpenAIRE

    Zúñiga García,D; EJ Pastén Castro; PA Araya-Díaz; Palomino Montenegro,H

    2012-01-01

    La aparatología empleada en los tratamientos de ortodoncia, favorece la retención de placa bacteriana y dificulta su eliminación por parte del paciente, aumentando el riesgo de desarrollar caries, manchas blancas y enfermedad periodontal. Diferentes cepillos dentales se han desarrollado para facilitar la mantención de una adecuada higiene oral en estos pacientes, sin embargo, se ha observado que los cepillos comunmente indicados (cepillo ortodóncico mas cepillo unipenacho), no siempre son bie...

  10. “VALORACIÓN DE PLACA BACTERIANA Y GINGIVITIS, MEDIANTE EL ÍNDICE DE PLACA DE O ́LEARY E ÍNDICE GINGIVAL DE LOE-SILNESS, POSTERIOR AL USO DE CLORHEXIDINA AL 0.12%, EN PACIENTES CON APARATOLOGIA FIJA”

    OpenAIRE

    Bucio Hernández, María Sugey

    2012-01-01

    Antecedentes: La búsqueda de agentes para el control de la placa bacteriana ha sido amplia, más teniendo en cuenta la importancia que ha adquirido la enfermedad periodontal en los últimos años; esto ha llevado a diversas industrias farmaceuticas a investigar en este campo. En el presente estudio se utilizo el índice Gingival de Löe y Silness (1963), por ser sencillo y valorar todas las áreas del diente, el más utilizado actualmente para medir el estado de inflamación y de salud...

  11. Efecto del consumo moderado del vino sobre la microbiota de la cavidad bucal

    OpenAIRE

    Tabasco, Raquel; García-Cayuela, Tomás; Muñoz-González, Irene; Moreno-Arribas, M. Victoria; Bartolomé, Begoña; Martínez-Cuesta, M. Carmen; Peláez, Carmen; Requena, Teresa

    2011-01-01

    La cavidad bucal está compuesta por cientos de especies de microorganismos diferentes, siendo en su mayoría bacterias, aunque aproximadamente sólo el 50% de estas especies se pueden cultivar. Diversos estudios han demostrado que existe un efecto entre los componentes del vino y la salud bucal, sin embargo, apenas existe información acerca de la modulación bacteriana por parte de los polifenoles del vino en la cavidad bucal. El objetivo de este trabajo se ha centrado en el estudio de los posib...

  12. Semen and reproductive profiles of genetically identical cloned bulls.

    Science.gov (United States)

    Tecirlioglu, R Tayfur; Cooney, Melissa A; Korfiatis, Natasha A; Hodgson, Renee; Williamson, Mark; Downie, Shara; Galloway, David B; French, Andrew J

    2006-06-01

    In this comparative study, reproductive parameters and semen characteristics of cloned bulls (n = 3) derived from somatic cell nuclear transfer (SCNT) were compared to their original cell donor Holstein-Friesian (n = 2) bulls from the same enterprise to assess the differences in reproductive potential between a donor bull and its clones. The parameters evaluated included motility of fresh, frozen-thawed and Percoll-treated frozen-thawed spermatozoa, as well as in vitro fertilization (IVF) ability, embryo quality, birth and survival of calves following IVF and embryo transfer with frozen-thawed semen. With fresh semen, spermatozoa from one cloned bull had lower motility than its donor. Cloned bulls had higher velocity parameters in fresh semen, but those effects were not obvious in frozen-thawed or frozen-thawed semen selected with a Percoll gradient. Semen collected from cloned bulls had significantly higher IVF rates compared to donors; however, embryo development per cleaved embryo or quality of blastocysts did not differ between donors and cloned bulls. Pregnancy and live offspring rates from one donor and its cloned bull did not differ between fresh (40%, 16/40 versus 46%, 17/37) and vitrified/thawed (13%, 2/16 versus 25%, 4/16) embryo transfer following IVF. A total of 26 calves were obtained from genotypically identical donor and cloned bulls with no signs of phenotypical abnormalities. These preliminary results suggested that the physiology of surviving postpubertal cloned bulls and quality of collected semen had equivalent reproductive potential to their original cell donor, with no evidence of any deleterious effects in their progeny.

  13. Aportes a la epidemiología de la meningitis bacteriana, Cuba:1998-2007

    OpenAIRE

    Dickinson Meneses, Félix Orlando

    2012-01-01

    En la presente investigación se abordó la epidemiología de la meningitis bacteriana en Cuba sobre la base de 10 años de vigilancia (1998-2007). Streptococcus pneumoniae fue el principal y más letal agente causal, que afectó sobre todo a las edades extremas de la vida y ocasionó una alta letalidad en los adultos y ancianos. La incidencia de la meningitis neumocócica y meningocócica se agrupó en algunos municipios de la región central. Esta última se mantuvo muy baja luego de 20 años de vacu...

  14. Piomiosite bacteriana aguda (PBA em crianças eutróficas

    Directory of Open Access Journals (Sweden)

    Mirtha B. Talavera

    2003-08-01

    Full Text Available A infecção aguda do músculo esquelético é denominada piomiosite bacteriana aguda (PBA. Ocorre mais freqüentemente em regiões tropicais e é mais prevalecente em crianças em idade escolar. A maioria dos casos relatados aconteceu em crianças desnutridas, com parasitoses. Relatamos quatro casos de PBA em crianças previamente eutróficas. A presença de dor muscular aguda localizada, com ou sem sintomas inflamatórios, ocorreu em todos os quatro casos. Osteomielite e/ou artrite séptica também ocorreram em todos os casos. O exame de escolha para o diagnóstico definitivo foi a ressonância nuclear magnética. A terapia foi realizada com antibioticoterapia endovenosa e drenagem cirúrgica dos abscessos.

  15. Nuevos métodos bacteriológicos para detectar y evitar la resistencia bacteriana

    Directory of Open Access Journals (Sweden)

    Margaret Ordoñez-Smith de Danies

    2003-09-01

    Full Text Available

    DEFINICIÓN DE LA RESISTENCIA

    De acuerdo al COC (Centro de Control de Enfermedades de Estados Unidos, la resistencia se da cuando un medicamento deja de inhibir el crecimiento o matar un microorganismo. 

    Hay dos tipos de resistencia la adquirida y la clínica. La adquirida se realiza en el laboratorio a través de las mutaciones o por transferencia de genes, sistemas de conjugación, transducción y transformación.

    La resistencia clínica es la observada en la práctica médica, donde no todos los organismos son igualmente sensibles o resistentes a cierto antibiótico y que requiere la ayuda de técnicas de laboratorio para medir cuantitativamente o cualitativamente dicho proceso.

    MECANISMOS DE LA RESISTENCIA BACTERIANA

    En la detección de la Resistencia Bacteriana se han identificado diferentes mecanismos utilizados por las bacterias: destrucción o inactivación enzimática, cambios en la permeabilidad de la membrana interna, alteraciones de los precursores de la pared celular, de la membrana y de los ribosomas.

    Para los años 90 se dispuso de una serie de antimicrobianos como betalactámicos, aminoglucósidos, macrólidos, sulfonamidas, trimetoprim, cloranfenicol, glicopéptidos, rifampicina, quinolonas, tetraciclinas....

     

  16. Semen quality in relation to antioxidant intake in a healthy male population

    DEFF Research Database (Denmark)

    Zareba, Piotr; Colaci, Daniela S; Afeiche, Myriam;

    2013-01-01

    To assess the relationship between dietary antioxidant intake and semen quality in young healthy males.......To assess the relationship between dietary antioxidant intake and semen quality in young healthy males....

  17. Maternal folic acid supplement intake and semen quality in Danish sons

    DEFF Research Database (Denmark)

    Jacobsen, Kristoffer; Ramlau-Hansen, Cecilia Høst; Thulstrup, Ane Marie

    2011-01-01

    To examine whether maternal folic acid supplement intake during pregnancy is related to better semen quality in male offspring.......To examine whether maternal folic acid supplement intake during pregnancy is related to better semen quality in male offspring....

  18. Propriedades físicas, químicas e de barreira em filme formados por blenda de celulose bacteriana e fécula de batata

    Directory of Open Access Journals (Sweden)

    Denise M. Almeida

    2013-01-01

    Full Text Available A associação da celulose bacteriana com outros polímeros pode ser um método efetivo para aumentar suas características e estrutura, proporcionando novas aplicações industriais. O objetivo do trabalho foi verificar a influência da celulose bacteriana nas propriedades físicas, químicas e de barreira em filmes a partir de blendas de celulose bacteriana e fécula de batata. Os filmes foram produzidos segundo planejamento de mistura ternária, tendo como variáveis independentes a celulose bacteriana, fécula de batata e glicerol. Blendas com maiores concentrações de celulose bacteriana apresentaram aumento na espessura, gramatura, taxa de permeabilidade ao vapor de água e permeabilidade ao vapor de água e, redução da densidade, solubilidade, opacidade, índice de intumescimento em pH 1,8 e 8,0 (1, 10, 30 e 60 minuto. A microscopia eletrônica de varredura mostrou filmes com matriz coesa, porém irregular com presença de partículas insolúveis de celulose bacteriana. O filme de celulose bacteriana pura apresentou aumento na gramatura, opacidade, taxa de permeabilidade ao vapor de água e no intumescimento em pH 1,8 1 minuto e pH 8,0 1, 10, 30 e 60 minutos. A celulose bacteriana promoveu elevada capacidade de retenção, absorção e baixa dessorção a água, proporcionando biofilmes com características de barreira à luz e manutenção da umidade.

  19. Deteksi Kerusakan DNA Spermatozoa Semen Segar dan Semen Beku Sapi Menggunakan Pewarnaan Toluidine Blue (DETECTION OF SPERM DNA DAMAGE INFRESH AND FROZEN SEMEN USING TOLUIDINE BLUE STAINING

    Directory of Open Access Journals (Sweden)

    Langgeng Priyanto

    2015-05-01

    Full Text Available Sperm DNA integrity is very important in the success of fertilization and embryo development.Toluidine blue (TB is a sensitive staining to examine the chromatin structure of sperm. The aim of thisstudy was to detect the sperm DNA damage before and after freezing using TB. Semen was obtained fromeight superior bulls (two Brahman, two Ongole, two Simental and two Limosin belong to Lembang ArtificialInsemination Centre. Semen was collected twice a week using artificial vagina, then was evaluatedmacrocopically and microscopically after collected, including sperm motility, viability, plasma membraneintegrity (MI and acrosome intact (AI, sperm concentration, abnormality and sperm DNA integrity. Thesemen that been used in this study showed the total motility was more than 70%, sperm concentrationwas more than 1000x106, and the sperm abnormality was below 20%. The result showed that the qualityof semen after freezing processed was decreased significantly (P<0.05 on percentage of sperm motility,viability, MI and AI, whereas there was no different on DNA integrity (P>0.05. Sperm DNA integrity offresh and frozen semen were 93.91±4.77% and 92.06 ±2.41% respectively. The decrease of DNA integritywas low (1.84% compared to motility (28.3%, viability (21.6%, MI (14.1%, and AI (11.8%. In conclusion,toluidine blue can be used to detect of DNA damage and the freezing process will not decrease the DNAintegrity.

  20. Semen characteristics in pubertal boys. IV. Semen quality and hormone profile.

    Science.gov (United States)

    Janczewski, Z; Bablok, L

    1985-01-01

    The biological quality of semen was contrasted with levels of FSH, LH, and testosterone in plasma. The level of FSH rises significantly from azoospermia/cryptozoospermia (3.87 +/- 1.1 mU/ml) to asthenozoospermia (5.73 +/- 2.11 mU/ml). In normospermia, however (4.63 +/- 1.88 mU/ml), the level of FSH decreases in a statistically significant manner and remains at the standard level. Comparing the level of LH to the quality of semen, it rises in a statistically significant manner from azoospermia/cryptozoospermia (6.46 +/- 1.35 mU/ml) to oligozoospermia (9.03 +/- 3.35 mU/ml). The level decreases in a statistically significant manner in normospermia (7.15 +/- 1.69 mU/ml). The level of testosterone shows a progressive linear growth from azoospermia/cryptozoospermia (6.03 +/- 2.09 micrograms/ml) to normospermia (6.55 +/- 2.12 micrograms/ml). The growth is statistically insignificant.

  1. ANALISIS PENERAPAN TAX PLANNING ATAS PAJAK PENGHASILAN BADAN PADA PT SEMEN TONASA PANGKEP

    OpenAIRE

    WIJAYA, AKBAR

    2014-01-01

    2014 ABSTRAK ANALISIS PENERAPAN TAX PLANNING ATAS PAJAK PENGHASILAN BADAN PADA PT SEMEN TONASA PANGKEP Implementation Analysis of Tax Planning on Board Incoming Tax of Semen Tonasa Ltd. Pangkep Akbar Wijaya Amiruddin Darmawati Penelitian ini bertujuan untuk mengetahui tax planning yang dilakukan oleh PT Semen Tonasa Pangkep, dan untuk menganalisis penerapan tax planning yang dilakukan oleh PT Semen Tonasa Pangkep dengan undang-undang perpajakan yang berlaku. Data...

  2. HIV-1 Populations in Semen Arise through Multiple Mechanisms.

    Directory of Open Access Journals (Sweden)

    Jeffrey A Anderson

    Full Text Available HIV-1 is present in anatomical compartments and bodily fluids. Most transmissions occur through sexual acts, making virus in semen the proximal source in male donors. We find three distinct relationships in comparing viral RNA populations between blood and semen in men with chronic HIV-1 infection, and we propose that the viral populations in semen arise by multiple mechanisms including: direct import of virus, oligoclonal amplification within the seminal tract, or compartmentalization. In addition, we find significant enrichment of six out of nineteen cytokines and chemokines in semen of both HIV-infected and uninfected men, and another seven further enriched in infected individuals. The enrichment of cytokines involved in innate immunity in the seminal tract, complemented with chemokines in infected men, creates an environment conducive to T cell activation and viral replication. These studies define different relationships between virus in blood and semen that can significantly alter the composition of the viral population at the source that is most proximal to the transmitted virus.

  3. Sensitive detection of Myobacterium avium subsp paratuberculosis in bovine semen by real-time PCR

    NARCIS (Netherlands)

    Herthnek, D.; Englund, S.; Willemsen, P.T.J.; Bolske, G.

    2006-01-01

    Aims: To develop a fast and sensitive protocol for detection of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine semen and to make a critical evaluation of the analytical sensitivity. Methods and Results: Processed semen was spiked with known amounts of MAP. Semen from different bulls as

  4. 9 CFR 98.35 - Declaration, health certificate, and other documents for animal semen.

    Science.gov (United States)

    2010-01-01

    ... establishments in which no case of scrapie had been confirmed during their residency; and (iii) Neither showed clinical signs of scrapie at the time of semen collection nor developed scrapie between the time of semen... not, affected with scrapie. (2) In the region where the semen originates: (i) Scrapie is...

  5. Relationship between age and semen parameters in men with normal sperm concentration: analysis of 6022 semen samples.

    Science.gov (United States)

    Levitas, E; Lunenfeld, E; Weisz, N; Friger, M; Potashnik, G

    2007-04-01

    This study evaluates retrospectively the relationship between age and semen parameters among men with normal sperm concentration. It was based on computerized data and performed in an Academic Fertility and IVF Unit. Six thousand and twenty-two semen samples with sperm concentrations of >or=20 x 10(6) ml(-1) were examined according to WHO criteria and analysed in relation to patients' age. For each age group, mean values +/- SD of semen volume, sperm concentration, percentage of motile spermatozoa, normal morphology, acrosome index, total sperm count/ejaculate, total motile sperm count/ejaculate and sexual abstinence duration were examined. A peak semen volume of 3.51 +/- 1.76 ml(-1) was observed at age >or=30 to age >or=55 years (PSperm motility was found to be inversely related to age with peak motility of 44.39 +/- 20.69% at age age >or=55 years (Psperm, between values of 103.34 +/- 107 x 10(6) at age >or=30 to age >55 years. A statistically significant and inverse relationship was observed between semen volume, sperm quality and patient age, in spite of prolonged sexual abstinence duration. Top sperm parameters were observed at age >or=30 to sperm parameters occurred after the age of 55 years.

  6. Rotation of Boar Semen Doses During Storage Affects Sperm Quality.

    Science.gov (United States)

    Schulze, M; Rüdiger, K; Waberski, D

    2015-08-01

    It is common practice to rotate boar semen doses during storage for prevention of sperm sedimentation. In this study, the effect of rotation of boar semen doses during storage on sperm quality was investigated. Manual turning twice daily and automatic rotation five times per hour resulted in the following effects: alkalinization of the BTS-extender, loss of membrane integrity at day 3, and loss of motility and changes in sperm kinematics during a thermoresistance test at day 5. Using a pH-stabilized variant of BTS extender, sperm motility and velocity decreased in continuously rotated samples, whereas membrane integrity and mitochondrial activity remain unaffected. It is concluded that rotation of semen samples adversely affects sperm quality and, therefore, should no longer be recommended for AI practice.

  7. [Semen analysis: spermiogram according to WHO 2010 criteria].

    Science.gov (United States)

    Gottardo, F; Kliesch, S

    2011-01-01

    Semen analysis plays a key role in the diagnostics of male infertility. Semen analysis has to be performed according to World Health Organisation (WHO) criteria. The updated version of the WHO manual was completed at the end of 2009 and published in 2010. Standard procedures in semen analysis include evaluation of sperm concentration, motility, morphology and vitality. In this new version particular attention has been paid to internal and external quality control, helping to identify and correct incidental and systematic errors both in routine analysis as well as in the field of research. The new manual describes all laboratory solutions, procedures and calculation formulas, and focuses on the definition of cryptozoospermia or azoospermia. A chapter concerning cryopreservation of spermatozoa has been newly integrated. The following overview presents the most important aspects of the updated WHO manual.

  8. Sperm penetration assay and its correlation with semen analysis parameters

    Directory of Open Access Journals (Sweden)

    Laxmi Kant Pandey

    2015-11-01

    Full Text Available Background: Aim of current study was to determine whether the Sperm Penetration Assay (SPA can be used as a test to discriminate the infertile male from fertile one. We have also correlated the SPA with semen analysis. Methods: Sperm characteristics namely Semen analysis and the sperm penetration assay were tested in 44 infertile and 10 fertile men. Sperm penetration assay was determined by using zona free hamster eggs. Results: With decreasing spermatozoa concentration in the semen there was significant decrease in percentage penetration of zona free Hamster eggs (p0.05. Conclusions: The Sperm penetration assay could discriminate the infertile group from fertile group significantly (p<0.001. The test appeared to be highly reproducible and probably identifies a truly infertile male. [Int J Res Med Sci 2015; 3(11.000: 3197-3201

  9. Aislamiento y selección de una cepa bacteriana degradadora de hidrocarburos a partir de suelos contaminados con petróleo

    Directory of Open Access Journals (Sweden)

    Rosa María Pérez Silva

    2008-01-01

    Full Text Available El conocimiento científico acerca del papel que desempeñan los microorganismos en el tratamiento de agentes contaminantes del medio ambiente como el petróleo, es esencial para prevenir y controlar los daños que puedan ocasionar los derrames o fugas de estos contaminantes. La degradación de petróleo es un proceso que puede ocurrir de forma natural por los microorganismos nativos de las zonas contaminadas aprovechando sus rutas metabólicas. Por esta razón, en estos momentos se prevé que los microorganismos pueden ofrecer esta posibilidad en tecnologías basadas en el uso de estos en la remediación de la contaminación ambiental por petróleo y sus derivados. En el presente trabajo, se estudió la biodegradación de petróleo por cepas aisladas de suelos contaminados con petróleo, mediante un aislamiento realizado por enriquecimiento secuencial utilizando petróleo Mesa 30/Puerto escondido (80 : 20 como única fuente de carbono y energía. Se aislaron 9 cepas bacterianas, cinco Gram negativas y cuatro Gram positivas, que fueron identificadas según el Manual Bergey's, 1994. Las pruebas bioquímicas evaluadas confirmaron que las cepas aisladas AT14, AT15, AT16, AT17 y AT18 corresponden a Pseudomonas aeruginosa. En este estudio, se seleccionó la cepa Pseudomonas aeruginosa AT18, por mostrar mayor crecimiento sobre petróleo como única fuente de carbono y energía al obtenerse 1,83 g/L de biomasa celular, lo que representa un 57 % de biodegradación de petróleo.

  10. Impact of pig insemination technique and semen preparation on profitability.

    Science.gov (United States)

    Gonzalez-Peña, D; Knox, R V; Pettigrew, J; Rodriguez-Zas, S L

    2014-01-01

    Artificial insemination technique and semen preparation impact boar utilization efficiency, genetic dissemination, and biosecurity. Intrauterine (IUI) and deep intrauterine (DUI) AI techniques require lower number of spermatozoa per dose compared to conventional (CON) AI. Frozen semen (FRO) has been associated with lower reproductive performance compared to fresh semen (FRE) preparation. The combined effects of 3 AI techniques (CON, IUI, and DUI) and 2 semen preparations (FRE and FRO) on the financial indicators of a pig crossbreeding system were studied. A 3-tier system was simulated in ZPLAN and the genetic improvement in a representative scenario was characterized. The cross of nucleus lines B and A generated 200,000 BA sows at the multiplier level. The BA sows were inseminated (CON, IUI, or DUI) with FRE or FRO from line C boars at the commercial level. Semen preparation and AI technique were represented by distinct sow:boar ratios in the C × BA cross. A range of farrowing rates (60 to 90%) and litter sizes (8 to 14 liveborn pigs) were tested. Genetic improvement per year for number born alive, adjusted 21-d litter weight, days to 113.5 kg, backfat, and ADG were 0.01 pigs per litter, 0.06 kg, -0.09 d, -0.29 mm, and 0.88 g, respectively. On average, the net profit for FRE (FRO) increased (P-value FRE and FRO were lower than -5%. The difference in variable costs between FRE and FRO ranged from -5.3 (CON) to -24.7% (DUI). Overall, insemination technique and semen preparation had a nonlinear effect on profit. The average relative difference in profit between FRE and FRO was less than 3% for the scenarios studied.

  11. Administração endovenosa de antibióticos e resistência bacteriana: responsabilidade da enfermagem

    Directory of Open Access Journals (Sweden)

    Heloisa Helena Karnas Hoefel

    2006-12-01

    Full Text Available O sucesso da terapêutica com antibióticos e o desenvolvimento da resistência bacteriana dependem de diversos fatores, sendo que os relacionados ao cuidado de enfermagem são o seu preparo e a sua administração. Objetivo: realizar uma revisão da literatura sobre falhas na administração de antibióticos analisando sua possível influência na resistência bacteriana. Delineamento: realizada uma pesquisa bibliográfica das fontes do LILACS e Medline. Métodos: realizada revisão bibliográfica sistematizada com pesquisa de artigos de 1994 a 2005 na Internet usando-se os seguintes descritores juntamente com enfermagem: resistência bacteriana, controle de antibióticos, infecções hospitalares, administração de medicamentos, erros de medicações e eventos adversos. Foram selecionados 58 artigos que apresentaram correlação com a enfermagem e/ou eram básicos na fundamentação brasileira ou internacional. Resultados: Foram descritos aspectos gerais sobre a resistência bacteriana, antibióticos e custos incluindo estratégias para prevenção da resistência e classificações de erros utilizadas internacionalmente. Conclusões: Baseado nesse conhecimento são recomendadas intervenções para implementação do cuidado de enfermagem prático e seguro.

  12. The extent of increase in first calving age as a result of implementing various sexed semen breeding strategies

    Directory of Open Access Journals (Sweden)

    Sahereh Joezy-Shekalgorabi

    2014-01-01

    Full Text Available A deterministic simulation was conducted to assess the effects of sexed semen utilization strategies on age at first calving (AFC. Four different strategies were implemented on dairy heifers: continuous use of conventional semen only (CC, continuous use of sexed semen only (SS, utilization of sexed semen for both the first and second services with conventional semen afterwards (S2, and utilization of sexed semen for the first service with conventional semen afterwards (S1. Results indicated that continuous utilization of sexed semen led to the greatest AFC; however at high conception rates, strategies displayed negligible differences on AFC. Increases in estrus detection rate had the greatest effects on decreasing AFC of the SS scenarios. Negative effect of sexed semen on AFC increased when the effect of low estrus detection rate was combined with low conception rate of sexed semen. Results indicated that in the case of access to sexed semen conception rate, prediction of AFC is possible by quadratic polynomial or exponential equations, depending to the applied breeding strategy. Simultaneous utilization of sexed and conventional semen in a herd did not make a substantial change in AFC when a low percentage of sexed semen was employed. Increasing the contribution of different sexed semen strategies led to higher AFC variation, especially for the SS strategy. AFC of strategies that utilize sexed semen is highly dependent on the conception rate, estrus detection rate and the contribution of sex sorted semen in the total number of inseminations of the heifer herd.

  13. Influence of chamber type integrated with computer-assisted semen analysis (CASA) system on the results of boar semen evaluation.

    Science.gov (United States)

    Gączarzewicz, D

    2015-01-01

    The objective of the study was to evaluate the effect of different types of chambers used in computer-assisted semen analysis (CASA) on boar sperm concentration and motility parameters. CASA measurements were performed on 45 ejaculates by comparing three commonly used chambers: Leja chamber (LJ), Makler chamber (MK) and microscopic slide-coverslip (SL). Concentration results obtained with CASA were verified by manual counting on a Bürker hemocytometer (BH). No significant differences were found between the concentrations determined with BH vs. LJ and SL, whereas higher (p0.05). The results obtained show that CASA assessment of boar semen should account for the effect of counting chamber on the results of sperm motility and concentration, which confirms the need for further study on standardizing the automatic analysis of boar semen.

  14. No increased sperm DNA fragmentation index in semen containing human papillomavirus or herpesvirus

    DEFF Research Database (Denmark)

    Kaspersen, Maja Døvling; Bungum, Mona; Fedder, Jens;

    2013-01-01

    It remains unknown whether human papillomaviruses (HPVs) or human herpesviruses (HHVs) in semen affect sperm DNA integrity. We investigated whether the presence of these viruses in semen was associated with an elevated sperm DNA fragmentation index. Semen from 76 sperm donors was examined by a PCR......-based hybridization array that identifies all HHVs and 35 of the most common HPVs. Sperm DNA integrity was determined by the sperm chromatin structure assay. HPVs or HHVs, or both, were found in 57% of semen samples; however, sperm DNA fragmentation index was not increased in semen containing these viruses....

  15. Effects of Vitamin E Addition to Chicken Semen on Sperm Quality During in Vitro Storage of Semen

    Directory of Open Access Journals (Sweden)

    Saleh Tabatabaei

    2011-06-01

    Full Text Available The purpose of this study was to evaluate the probable effects of the vitamin E addition in different levels to the extender of chicken semen on spermatozoa quality during storage of semen at 4°C for 0, 3, 6, 10 and 24 hours. Eight young Ross broiler breeder strain 308 roosters were used in this experiment. The collected semen from all roosters was mixed together and diluted with modified a Ringer’s solution. The diluted pooled semen was divided into 5 treatments (T. T1 was a control group without any vitamin E addition. For T2 to T5 groups 0.5 %, 1 %, 2 % and 3 % vitamin E (w/v, were added respectively. Treatments were evaluated for sperm motility, sperm viability and probable morphological defects after 0, 3, 6, 10 and 24 hours of incubation at 4°C. The evaluations of spermatozoa immediately after semen collection, were revealed no significant differences among values of treatment groups, whereas after incubating the treatments for different spans of time, the sperm progressive motility and viability rates for groups supplemented with vitamin E were significantly (P < 0.05 higher than that of the control group. In addition, morphological defect rates of chicken spermatozoa in the groups supplemented with different levels of vitamin E were significantly (P < 0.05 lower than that in control group. According to the results of this study we conclude that, the most excellent level of vitamin E for supplementation to the extended semen of chicken in order to improve the sperm motility and viability plus to reduce the morphological defect rates of the spermatozoa up to 24 hours storage time at 4°C is 2 % (w/v.

  16. Medición de cambios cuantitativos de la microbiota subgingival posterior a la remoción de placa bacteriana supragingival Measurement of quantitative changes of the microbiota subgingival after to removal of bacterial plaque supragingival

    Directory of Open Access Journals (Sweden)

    C Godoy

    2010-04-01

    Full Text Available Uno de los campos de interés en el estudio de la microbiología periodontal para muchos investigadores ha sido identificar si es posible que los microorganismos responsables del origen y progresión de la enfermedad periodontal que habitan sobre el margen gingival (supragingival y bajo este (subgingival tengan una relación directa que permita mantener interacciones influyentes en el crecimiento y desarrollo de las diferentes especies bacterianas que habitan en los tejidos periodontales. Por lo tanto, al remover los microorganismos que se ubican supragingivalmente sería posible encontrar cambios en el medio subgingival al no existir un intercambio entre los ambientes aerobios (supragingival y anaerobios (subgingival una vez desorganizada la placa bacteriana supragingival. Para demostrar esta relación se seleccionaron 7 individuos con diagnóstico de periodontitis crónica moderada y severa a los cuales se les realizó un destartraje supragingival de boca completa para lograr desorganizar la placa bacteriana supragingival. A su vez se tomaron muestras microbiológicas de los sacos periodontales más profundos de cada cuadrante de estos individuos, siendo la primera muestra tomada previo al destartraje supragingival considerada como muestra basal (día 0, luego se tomaron a las 24 horas, a los 7 y 21 días de removida la placa bacteriana supragingival. De los resultados del presente estudio pudimos concluir que al desorganizar el biofilm supragingival se observa una disminución en la cantidad total de microorganismos subgingivales, así como también disminuye de manera considerable la proporción de Porphyoromona gingivalis presente en el medio subgingival. Lo cual permitiría establecer la existencia de una relación directa y dependiente entre los microorganismos que habitan el medio supragingival y subgingival.One of the fields of interest in the study of the microbiology periodontal for many investigators has been to identify if it is

  17. Efecto del cobre y del cadmio sobre la respuesta inmune innata del pez Colossoma macropomum

    OpenAIRE

    2012-01-01

    En este trabajo se evaluó la respuesta inmunológica celular inespecífica del pez Colossoma macropomum (Cuvier, 1818) expuesto a concentraciones subletales de cobre y cadmio, a través de la determinación de la viabilidad celular, la quimiotaxis, la fagocitosis y la muerte bacteriana en células inmunocompetentes extraídas del riñón cefálico, la sangre y el hígado. Los peces fueron expuestos por separado a 0,5 mg/L de cloruro de cobre y 1,0 mg/L de cloruro de cadmio durante 28 días; después de l...

  18. New methods and media for the centrifugation of honey bee (Hymenoptera: Apidae) drone semen.

    Science.gov (United States)

    Wegener, Jakob; May, Tanja; Kamp, Günter; Bienefeld, Kaspar

    2014-02-01

    Centrifugation of Apis mellifera L. drone semen is a necessary step in the homogenization of semen pools for the enlargement of the effective breeding population, as well as in the collection of semen by the so-called washing technique. It is also of interest for the removal of cryoprotectants after cryopreservation. The adoption of methods involving semen centrifugation has been hampered by their damaging effect to sperm. Here, we tested four new diluents as well as three additives (catalase, hen egg yolk, and a protease inhibitor), using sperm motility and dual fluorescent staining as indicators of semen quality. Three of the new diluents significantly reduced motility losses after centrifugation, as compared with the literature standard. Values of motility and propidium iodide negativity obtained with two of these diluents were not different from those measured with untreated semen. The least damaging diluent, a citrate-HEPES buffer containing trehalose, was then tested in an insemination experiment with centrifuged semen. Most queens receiving this semen produced normal brood, and the number of sperm reaching the storage organ of the queen was not significantly different from that in queens receiving untreated semen. These results could improve the acceptance of techniques involving the centrifugation of drone semen. The diluent used in the insemination experiment could also serve as semen extender for applications not involving centrifugation.

  19. Apoptosis induction of Persicae Semen extract in human promyelocytic leukemia (HL-60) cells.

    Science.gov (United States)

    Kwon, Hee-Young; Hong, Seon-Pyo; Hahn, Dong-Hoon; Kim, Jeong Hee

    2003-02-01

    The major ingredient of Persicae Semen is a cynogenic compound, amygdalin (D-mandelonitrile-beta-gentiobioside). Controversial results on the anticancer activity of amygdalin were reported due to its conversion to its inactive isomer, neoamygdalin. In order to inhibit the epimerization of amygdalin, we used newly developed simple acid boiling method in preparation of Persicae Semen extract. HPLC analysis revealed most of amygdalin in Persicae Semen extract was active D-form. Persicae Semen extract was used to analyze its effect on cell proliferation and induction of apoptosis in human promyelocytic leukemia (HL-60) cells. Persicae Semen extract was cytotoxic to HL-60 cells with IC50 of 6.4 mg/mL in the presence of 250 nM of beta-glucosidase. The antiproliferative effects of Persicae Semen extract appear to be attributable to its induction of apoptotic cell death, as Persicae Semen extract induced nuclear morphology changes and internucleosomal DNA fragmentation.

  20. Maintaining semen quality by improving cold chain equipment used in cattle artificial insemination

    Science.gov (United States)

    Lieberman, Daniel; McClure, Elizabeth; Harston, Stephen; Madan, Damian

    2016-06-01

    Artificial insemination of dairy cattle is a common practice in the developing world that can improve farmer incomes and food security. Maintaining the fertilizing potential of frozen semen as it is manipulated, transported and stored is crucial to the success of this process. Here we describe simple technological improvements to protect semen from inadvertent thermal fluctuations that occur when users mishandle semen using standard equipment. We show that when frozen semen is mishandled, characteristics of semen biology associated with fertility are negatively affected. We describe several design modifications and results from thermal performance tests of several improved prototypes. Finally, we compare semen that has been mishandled in standard and improved equipment. The data suggest that our canister improvements can better maintain characteristics of semen biology that correlate with fertility when it is mishandled.

  1. Do Ureaplasma urealyticum infections in the genital tract affect semen quality?

    Institute of Scientific and Technical Information of China (English)

    Yan Wang; Cui-Ling Liang; Jun-Qing Wu; Chen Xu; Shi-Xiao Qin; Er-Sheng Gao

    2006-01-01

    Aim: To investigate the relationship between Ureaplasma urealyticum (UU) infection and semen quality. Methods:From 2001 to 2003, 346 eligible patients aged 20-45 years were invited from two hospitals in Shanghai, China, to participate in an investigation which included questionnaires about general and reproductive health, an external genital tract examination, UU culture and semen analysis. Multiple linear regression models were used to examine whether UU had a significant effect on semen quality after adjustment for confounding factors. Results: Findings suggested that UU infection was associated with higher semen viscosity and lower semen pH value. Sperm concentration was lower in UU positive subjects than that in UU negative subjects (54.04 × 106/mL vs.70.58 × 106/mL). However, Uudid not significantly affect other semen quality indexes. Conclusion: UU infection of the male genital tract could negatively influence semen quality.

  2. Reproduction in nondomestic birds: Physiology, semen collection, artificial insemination and cryopreservation

    Science.gov (United States)

    Gee, G.F.; Bertschinger, H.; Donoghue, A.M.; Blanco, J.; Soley, J.

    2004-01-01

    Pioneering work by Quinn and Burrows in the late 1930s led to successful artificial insemination (AI) programs in the domestic poultry industry. A variety of species specific modifications to the Quinn and Burrows massage technique made AI possible in nondomestic birds. Massage semen collection and insemination techniques span the entire range of species from sparrows to ostriches. Also, cooperative semen collection and electroejaculation have found limited use in some nondomestic species. Artificial insemination produces good fertility, often exceeding fertility levels in naturally copulating populations. However, aviculturists should explore other ways to improve fertility before resorting to AI. Artificial insemination is labor intensive and may pose risks to nondomestic birds as well as handlers associated with capture and insemination. Semen collection and AI makes semen cryopreservation and germ plasma preservation possible. Yet, semen cryopreservation techniques need improvement before fertility with frozen-thawed semen will equal fertility from AI with fresh semen.

  3. Insights into semen analysis:a Chinese perspective on the fifth edition of the WHO laboratory manual for the examination and processing of human semen

    Institute of Scientific and Technical Information of China (English)

    Wen-Hong Lu; Yi-Qun Gu

    2010-01-01

    @@ Dear Editor, We are very glad to see that the Asian Journal of Andrology published a Special Issue on Semen Analysis in 21st Century Medicine, which well revealed some behind-the-scene controversies of the 5th edition of the WHO laboratory manual for the examination and processing of human semen [1]. Three articles from the special issue, two on the reference values of semen parameters [2,3] and another presenting the investigation results of 118 laboratories performing semen analysis in Mainland China [4], are very thoughtprovoking and we would like to share some of our views on these topics.

  4. Correlation between the neonatal EEG and the neurological examination in the first year of life in infants with bacterial meningitis Correlación entre el EEG neonatal y el examen neurológico en el primer año de vida en recién nacidos con meningitis bacteriana

    Directory of Open Access Journals (Sweden)

    Adrián Poblano

    2007-09-01

    Full Text Available OBJECTIVE: To assess the contribution of neonatal electroencephalogram (EEG and its correlation with the neurological examination at age of 9 months in newborns with bacterial neonatal meningitis. METHOD: Twenty seven infants were studied with positive cerebrospinal fluid (CSF culture for bacteria. We used the worse EEG result during acute phase of meningitis, and performed neurologic follow-up after discharge from hospital. Background cerebral activity was classified as normal or mildly, moderately, or markedly abnormal. Neurologic examination outcomes was classified normal, mild abnormalities, moderate abnormalities and severe abnormalities. RESULTS: EEG performed in the neonatal period during acute bacterial meningitis predicts adverse outcome early at age of 9 months, and had a significant correlation with cephalic perimeter and active tone alterations. CONCLUSION: Neonatal EEG is useful for predicting abnormal outcomes, especially cephalic perimeter and active tone abnormalities at 9 months of age in infants with bacterial neonatal meningitis.OBJETIVO: Medir la contribución del electroencefalograma (EEG neonatal y su correlación con el examen neurológico a la edad de 9 meses en recién nacidos con meningitis neonatal bacteriana. MÉTODO: Se estudió a 27 neonatos con cultivos positivos de líquido cefalorraquídeo a bacterias. Se uso el peor resultado del EEG obtenido durante el periodo agudo de la meningitis. El seguimiento neurológico se efectuó tras el egreso hospitalario. La actividad de fondo del EEG se clasificó en normal y anormal leve, moderada y severa. El examen neurológico se clasificó en normal, y anormal leve moderado y severo. RESULTADOS: El EEG realizado durante el periodo neonatal durante la fase aguda de la meningitis bacteriana predice bien un resultado adverso a la edad de 9 meses, con correlaciones significativas con el perímetro cefálico y con las alteraciones del tono activo. CONCLUSION: El EEG neonatal es

  5. Influence of addition of different antibiotics in semen diluent on viable bacterial count and spermatozoal viability of Awassi ram semen

    Directory of Open Access Journals (Sweden)

    O I Azawi

    2012-04-01

    Full Text Available The objectives of the present study were to determine the effects of six different antibiotics in controlling the growth of semen contaminating bacteria and if these antibiotics have any adverse effect on Awassi ram spermatozoa. Semen samples from six mature Awassi rams were used in this study. A total number of 120 ejaculates were collected from the rams using an artificial vagina once a week. Semen ejaculates were evaluated for volume, sperm concentration, mass motility, individual motility, percentage live sperm, sperm abnormalities, and viable bacterial count. Semen samples were diluted by sodium citrate-fructose-egg yolk. The diluted semen sample was divided into 7 parts. Six types of antibiotics were added to the semen diluent parts including; penicillin G 1000 IU ml-1 with streptomycin 1 mg ml-1, gentamicin sulphate 250 mg ml-1, tetracycline 0.5 mg ml-1, lincomycin 1 mg ml-1, cefoperazone sodium 1mg ml-1, cefdinir 1 mg ml-1 and the seventh part considered as a control group without antibiotic addition. The diluted semen samples were cooled and preserved at 5 Co for 5 days. Cooled diluted semen samples were examined for individual motility, percent of live sperm, sperm abnormalities, acrosomal defects and bacterial count every 24 h until 5 days. Comparing with the control, all the antibiotics examined were effective in controlling bacterial growth (P<0.05 from 24 h to 96 h of preservation at 5 Co. Cefdinir and cefoperazone sodium proved to be significantly (P<0.05 effective than other antibiotics in controlling bacterial growth at 96 h of preservation as the bacterial count were 23.3 ± 3.7 x 103 / ml and 25.4 ± 6.2 x 103 / ml, respectively. Lincomycin, gentamicin sulphate and tetracycline proved ineffective in controlling bacterial growth at 96 h of preservation as the bacterial count were 57.1 ± 20.1 x 103 / ml, 52.5 ± 29.4 x 103 / ml and 46.5 ± 8.8 x 103 / ml, respectively. The addition of tetracycline to diluted ram semen

  6. Preservation of mithun (Bos frontalis) semen at refrigeration temperature.

    Science.gov (United States)

    Karunakaran, M; Dhali, A; Mech, A; Khate, K; Rajkhowa, C; Mishra, D P

    2007-10-01

    The objective of the present study was to investigate the possibility of preserving mithun (Bos frontalis) spermatozoa at refrigeration temperature using tris-egg yolk diluent. Semen samples were collected from four adult mithun bulls through rectal massage method. Good quality semen samples (n=30) were preserved at 4 degrees C using tris-egg yolk diluent for 72 h. Progressive motility, live spermatozoa count and morphological abnormalities were evaluated every 12 h until 72 h of preservation. The colour, consistency and mass activity of fresh semen samples were found to be creamy white, medium and 3+ to 4+ (5+ scale), respectively. The average (mean+/-S.E.) volume (ml), pH and spermatozoa concentration (10(6) ml(-1)) of fresh semen samples were found to be 0.6+/-0.01, 6.8+/-0.03 and 425+/-48, respectively. Progressive motility and live spermatozoa count were found to be less than 30% (Prefrigeration temperature in tris-egg yolk diluent, which can be further used for artificial insemination within 36 h of storage.

  7. Association of Sleep Disturbances With Reduced Semen Quality

    DEFF Research Database (Denmark)

    Jensen, Tina Kold; Andersson, Anna-Maria; Skakkebæk, Niels Erik;

    2013-01-01

    Several studies have found an association between sleep duration and morbidity and mortality, but no previous studies have examined the association between sleep disturbances and semen quality. We conducted a cross-sectional study among 953 young Danish men from the general population who were re...

  8. Complete Genome Sequence of Zika Virus Isolated from Semen

    Science.gov (United States)

    Graham, Victoria; Lewandowski, Kuiama; Dowall, Stuart D.; Pullan, Steven T.; Hewson, Roger

    2016-01-01

    Zika virus (ZIKV) is an emerging pathogenic flavivirus currently circulating in numerous countries in South America, the Caribbean, and the Western Pacific Region. Using an unbiased metagenomic sequencing approach, we report here the first complete genome sequence of ZIKV isolated from a clinical semen sample. PMID:27738033

  9. Effect of air-conditioner exposure on semen quality

    Institute of Scientific and Technical Information of China (English)

    Min-LiLu; Jun-QingWu; Qiu-YingYang; Wei-JinZhou; Er-ShengGao

    2004-01-01

    Aim: To investigate the effect of air-conditioner exposure on semen quality. Methods: The data came from the healthy male volunteers, aged 22 to 30 years, who went to centers for maternity and children health for premarital physical examination in Shanghai,Henan, Zbejiang and Hebei from December 1998 to February 2000.

  10. Effect of various levels of catalase antioxidant in semen extenders on lipid peroxidation and semen quality after the freeze-thawing bull semen

    Directory of Open Access Journals (Sweden)

    Reza Asadpour

    2011-11-01

    Full Text Available The objective of this study was to evaluate effect of different concentrations of catalase in two extenders on motility, viability and lipid peroxidation bull spermatozoa during semen freezing process. Thirty ejaculates collected from ten Holstein bulls were pooled and evaluated at 37 °C. Pool ejaculated was split into two main experimental groups, 1 and 2. In experiment 1, specimen was diluted to a final concentration of 30 × 106 spermatozoa with citrate-egg yolk and in experiment 2; specimen was diluted with tris-egg yolk extender to the same concentration. In both experiments diluted semen was divided into three aliquots, including a control and two test groups. Each aliquot was rediluted with an equal volume of extender either without (control or with one of the antioxidants contained one of the following antioxidants: catalase (CAT; 100 IU mL-1 catalase (CAT; 200 IU mL-1 and control group. No significant differences were observed in sperm viability and motility following addition of catalase enzyme at concentration of 100 IU mL-1 and 200 IU mL-1 to citrate-egg yolk extender. But the highest sperm viability was achieved by addition of 100 IU mL-1 and 200 IU mL-1 catalase to tris-egg yolk semen extender compared with the control group (P < 0.05. Malondialdehyde levels did not change with addition of catalase in both extenders compared with the control group. The obtained results provide a new approach to the cryopreservation of bull semen, and could positively contribute to intensive cattle production.

  11. Estimación de poblaciones de microorganismos del ciclo del nitrógeno y su relación con el grado de eutroficación del humedal Jaboque, Cundinamarca, Colombia

    OpenAIRE

    Valencia Hernando; Rodríguez Alejandro A.

    2006-01-01

    Se investigaron las poblaciones bacterianas del ciclo del nitrógeno relacionadas con procesos de descomposición
    y mineralización de la materia orgánica y grado de eutrofización del humedal Jaboque. En general se
    encontró un predominio de las poblaciones de bacterias amonificantes en los diferentes muestreos y zonas
    descritas. Éstas realizan la mineralización de la materia orgánica y su gran actividad se relaciona con el alto
    grado de eutroficación d...

  12. A RIFAMPICINA NA DESCONTAMINAÇÃO BACTERIANA DE EXPLANTES DE MAMOEIRO PROVENIENTES DO CAMPO

    Directory of Open Access Journals (Sweden)

    GIOVANNI RODRIGUES VIANNA

    1997-01-01

    Full Text Available Observou-se alta contaminação bacteriana nos explantes de mamoeiro introduzidos in vitro, a partir de plantas matrizes desenvolvidas no campo, independentemente da época do ano em que se realizaram as coletas. O uso de desinfestantes superficiais, como álcool e hipoclorito de sódio, garantiram níveis aceitáveis de controle apenas para fungos, não para bactérias. A rifampicina, por tratamento de imersão ou introdução em meio de cultura, controlou satisfatoriamente as contaminações de caráter endofítico, obtendo-se 70% de explantes sadios, sem sinais de fitotoxicidade.High contamination by bacteria was observed in papaya tissue cuttings introduced in vitro from plants grown in the field, independent of the period of the year that samples were collected. The use of alcohol and sodium hypoclorite did not guarantee good bacteria control. Rifampicin, added as an immersion solution treatment or in the culture media, controlled the internal contamination of explants, without damaging the cuttings. Up to 70% of healthy tissue explants were obtained by the use of rifampicin.

  13. Infecções bacterianas tratadas com Ro 6-2580

    Directory of Open Access Journals (Sweden)

    Paulo F. A. Lopes

    1970-10-01

    Full Text Available Nova combinação medicamentosa composta de sulfametoxazól e trimetoprim ("Bactrim", com efeito bactericida sobre germes Gram negativos e Gram positivos, foi ensaiada no tratamento de 15 casos de salmonelose e 9 casos de infecções bacterianas. Os resultados foram favoráveis, tanto nas salmoneloses, como em infecções por estafilococos, shigelas, pneumococos e coliformes. A remissão do estado tóxico nos casos de febre tifóide foi observada dentro de 48 horas, e o tempo médio para desaparecimento da febre e demais sintomas, nessa infecção, foi de 3 dias. Foram constatados efeitos secundários em alguns pacientes, tais como hipertermia medicamentosa, farmacodermia, vômitos, linfomonocitose e eosinofilia, possivelmente relacionados a posologia excessiva, e todos remissíveis. Os autores concluem pela utilidade do novo medicamento na terapêutica de salmoneloses, estafilococcias e outras infecções.

  14. Screening for Sexually Transmitted Infection Pathogens in Semen Samples

    Directory of Open Access Journals (Sweden)

    RW Peeling

    2005-01-01

    Full Text Available The transmission of sexually transmitted infection (STI pathogens from an infected donor to the recipient of a semen donation in assisted conception may result not only in acute infection but also in long-term reproductive complications or adverse outcomes of pregnancy, including infection of the offspring. Screening for bacterial STI pathogens, Chlamydia trachomatis and Neisseria gonorrhoeae is strongly recommended because these pathogens can cause serious reproductive complications in the recipients of semen donations and infection in their offspring. Screening for these pathogens should be performed using the most sensitive methods, such as nucleic acid amplified tests. False-negative results due to inhibitory substances in the semen sample should be monitored using amplification controls. Where specimen transport is not a problem and culture facilities are available, N gonorrhoeae can also be detected by culture. Laboratories performing screening should subscribe to proficiency programs and have strict quality controls. Although Trichomonas vaginalis, group B streptococcus and genital mycoplasmas have been associated with adverse outcomes of pregnancy, the frequent finding of these organisms in healthy individuals brings into question the validity of mandatory inclusion of these organisms in the screening panel. Although viral STI pathogens and Treponema pallidum -- the causative agent of syphilis -- may be detected in semen, their presence may be more sensitively detected through antibody testing of the donor. Screening donors for HIV, hepatitis B and syphilis by serology is uniformly recommended in all of the guidelines, but the value of screening either donors or semen samples for cytomegalovirus, herpes simplex viruses and human papilloma viruses is less clear.

  15. Screening for sexually transmitted infection pathogens in semen samples.

    Science.gov (United States)

    Peeling, Rw; Embree, J

    2005-03-01

    The transmission of sexually transmitted infection (STI) pathogens from an infected donor to the recipient of a semen donation in assisted conception may result not only in acute infection but also in long-term reproductive complications or adverse outcomes of pregnancy, including infection of the offspring. Screening for bacterial STI pathogens, Chlamydia trachomatis and Neisseria gonorrhoeae is strongly recommended because these pathogens can cause serious reproductive complications in the recipients of semen donations and infection in their offspring. Screening for these pathogens should be performed using the most sensitive methods, such as nucleic acid amplified tests. False-negative results due to inhibitory substances in the semen sample should be monitored using amplification controls. Where specimen transport is not a problem and culture facilities are available, N gonorrhoeae can also be detected by culture. Laboratories performing screening should subscribe to proficiency programs and have strict quality controls. Although Trichomonas vaginalis, group B streptococcus and genital mycoplasmas have been associated with adverse outcomes of pregnancy, the frequent finding of these organisms in healthy individuals brings into question the validity of mandatory inclusion of these organisms in the screening panel. Although viral STI pathogens and Treponema pallidum - the causative agent of syphilis - may be detected in semen, their presence may be more sensitively detected through antibody testing of the donor. Screening donors for HIV, hepatitis B and syphilis by serology is uniformly recommended in all of the guidelines, but the value of screening either donors or semen samples for cytomegalovirus, herpes simplex viruses and human papilloma viruses is less clear.

  16. EFFECT OF TWO PROTOCOLS OF CRYOPRESERVATION ON FERTILIZING CAPACITY OF STALLION (Equus caballus SEMEN EFECTO DE DOS PROTOCOLOS DE CRIOPRESERVACIÓN SOBRE LA CAPACIDAD FECUNDANTE DE SEMEN EQUINO (Equus caballus

    Directory of Open Access Journals (Sweden)

    Giovanni Restrepo Betancur

    2012-06-01

    soportes para el almacenamiento del semen, se ha constituido en una alternativa para mejorar los protocolos empleados. El objetivo de este trabajo fue evaluar el efecto de dos protocolos de criopreservación (congelación y vitrificación, sobre la capacidad fecundante del semen equino. El estudio se realizó con equinos de la raza Criollo Colombiano. Para la congelación se empleó un diluyente suplementado con de yema de huevo (4% y dimetilformamida (5%, y pajillas de 0,5 mL como soportes; mientras que para la vitrificación, el diluyente fue suplementado con yema de huevo (8% y dimetilformamida (8% y se usaron crioviales como soportes. Post-descongelación, se evaluaron los parámetros: movilidad progresiva, vitalidad, morfología normal e integridad de la membrana plasmática (HOS. Para la evaluación estadística se ajustó un modelo lineal generalizado (GLM y las medias se compararon por la prueba de Tukey. Se encontraron porcentajes promedio de movilidad progresiva, vitalidad, morfología normal y HOS de 41,6±11,8 y 37,0±8,5, 54,3±10,2 y 52,3±7,8, 83,1±5,4 y 83,6±5,8, 41,7±9,8 y 38,9±3,6, para el semen criopreservado por congelación y vitrificación, respectivamente. No se encontraron diferencias estadísticamente significativas (P ≤ 0,05 entre los tratamientos para ninguno de los parámetros evaluados. La capacidad fecundante del semen equino criopreservado por vitrificación es equiparable a la obtenida por congelación convencional.

  17. Supplementing oregano essential oil to boar diet with strengthened fish oil: Effects on semen antioxidant status and semen quality parameters.

    Science.gov (United States)

    Liu, Q; Duan, R J; Zhou, Y F; Wei, H K; Peng, J; Li, J L

    2017-02-22

    Previous research has shown benefits of dietary fish oil supplementation on semen quality of boars. However, little is known about how antioxidant protects lipid peroxidation on spermatozoa from n-3 polyunsaturated fatty acid (PUFA) addition. This study evaluated the effect of oregano essential oil (OEO) supplementation on semen antioxidant status and semen quality in boars fed a diet enriched with fish oil. Thirty-four mature boars of proven fertility, received daily 2.5 kg basal diet top-dressed with 45 g soybean oil and 15 g fish oil to meet the n-3 PUFA requirement of spermatozoa, randomly allocated to one of four groups supplemented with 100 mg α-tocopheryl acetate kg(-1) (control), or 250 or 500 or 750 mg OEO kg(-1) for 16 weeks. Semen was collected at weeks 0, 8, 12 and 16 for measurements of sperm production, motion characteristics, sperm α-tocopherol content, antioxidant enzyme activities, reactive oxygen species (ROS), DNA damage (8-hydroxydeoxyguanosine, 8-OHdG), lipoperoxidation (malondialdehyde, MDA) and seminal total antioxidant capacity (TAC). Sperm production and motion characteristics were similar (p > .05) among groups throughout the experimental week 16, but increased (p oil has a positive effect on antioxidant capacity in boar when used fish oil.

  18. CRIOCONSERVACIÓN DE SEMEN EN PECES: EFECTOS SOBRELA MOVILIDAD ESPERMÁTICA Y LA FERTILIDAD. Semen Cryopreservation in Fish: Effects on Sperm Motility and Fertility.

    Directory of Open Access Journals (Sweden)

    JOSÉ GREGORIO MARTÍNEZ

    Full Text Available La crioconservación de semen de peces, como de otras especies, presenta aún efectos que disminuyen la calidad espermática y comprometen directamente la capacidad de la célula para participar exitosamente en los procesos de fertilización y desarrollo embrionario. Características como movilidad y capacidad de fertilización del espermatozoide son consideradas criterios de calidad que permiten medir el éxito o fracaso del proceso, pues se consideran variables integradoras, siendo indicadores que dependen no de un solo factor sino de la estabilidad y bienestar del conjunto de estructuras, enzimas y compuestos funcionales subcelulares que dan lugar a estas características espermáticas. Daños en la membrana (adenilato ciclasa, canales iónicos, agrupamiento de otras proteínas, entre otras y su implicación en la ruta de señalización que da lugar a la activación espermática, degradación del ATP, fragmentación del ADN nuclear y mitocondrial (genoma, degradación de enzimas Kinasas y otras proteínas citosólicas (proteoma son considerados hoy día como algunos de los factores moleculares que más se afectan durante la crioconservación y que disminuyen ostensiblemente la capacidad fertilizante y la movilidad del espermatozoide en los peces. Propuestas sobre los mecanismos moleculares por los cuales se interrelacionan y actúan estos factores subcelulares como consecuencia de la crioconservación, son algunos de los temas tratados en la presente revisión. Comprender los principios y factores que están involucrados en el origen de dichos daños, permitirá mejorar los procesos de crioconservación, haciéndolos menos nocivos y más eficientes.The cryopreservation of semen in fish, as in many species even shows effects that decrease sperm quality and directly engage cell ability to successfully participate in the processes of fertilization and embryonic development. The characteristics such as mobility and fertilizing capacity of

  19. Critical sources of bacterial contamination and adoption of standard sanitary protocol during semen collection and processing in Semen Station

    Directory of Open Access Journals (Sweden)

    Chandrahas Sannat

    2015-05-01

    Full Text Available Aim: The present investigation was conducted to locate the critical sources of bacterial contamination and to evaluate the standard sanitation protocol so as to improve the hygienic conditions during collection, evaluation, and processing of bull semen in the Semen Station. Materials and Methods: The study compared two different hygienic procedures during the collection, evaluation and processing of semen in Central Semen Station, Anjora, Durg. Routinely used materials including artificial vagina (AV inner liner, cone, semen collection tube, buffer, extender/diluter, straws; and the laboratory environment like processing lab, pass box and laminar air flow (LAF cabinet of extender preparation lab, processing lab, sealing filling machine, and bacteriological lab were subjected to bacteriological examination in two phases of study using two different sanitary protocols. Bacterial load in above items/environment was measured using standard plate count method and expressed as colony forming unit (CFU. Results: Bacterial load in a laboratory environment and AV equipments during two different sanitary protocol in present investigation differed highly significantly (p<0.001. Potential sources of bacterial contamination during semen collection and processing included laboratory environment like processing lab, pass box, and LAF cabinets; AV equipments, including AV Liner and cone. Bacterial load was reduced highly significantly (p<0.001 in AV liner (from 2.33±0.67 to 0.50±0.52, cone (from 4.16±1.20 to 1.91±0.55, and extender (from 1.33±0.38 to 0 after application of improved practices of packaging, handling, and sterilization in Phase II of study. Glasswares, buffers, and straws showed nil bacterial contamination in both the phases of study. With slight modification in fumigation protocol (formalin @600 ml/1000 ft3, bacterial load was significantly decreased (p<0.001 up to 0-6 CFU in processing lab (from 6.43±1.34 to 2.86±0.59, pass box (from 12

  20. Effect of semen extender and density gradient centrifugation on the motility and fertility of turkey spermatozoa.

    Science.gov (United States)

    Morrell, J M; Persson, B; Tjellström, H; Laessker, A; Nilsson, H; Danilova, M; Holmes, P V

    2005-12-01

    In the absence of commercially viable methods for cryopreserving turkey spermatozoa, new processing methods are required to extend the functional life of stored turkey spermatozoa for artificial insemination. The present study evaluates the efficacy of a new extender (Turkey Semen Extend) and investigates the use of density gradient centrifugation in processing turkey spermatozoa for artificial insemination. The new extender is compared with two commercially available turkey semen extenders, Beltsville Poultry Semen Extender and Ovodyl. Turkey spermatozoa in Turkey Semen Extend were still motile 20 h after collection, representing a considerable improvement over the other semen extenders (40%, 0% and 8% for Turkey Semen Extend, Beltsville Poultry Semen Extender and Ovodyl, respectively). A field trial on a commercial turkey farm showed improved fertilization rates following insemination of turkey hens with semen extended in Turkey Semen Extend (89.7%) compared with Beltsville Poultry Semen Extender (86.9%). This difference is statistically significant (p < 0.05). Processing on a density gradient, optimized for turkey spermatozoa, also increased sperm survival (50% gradient-prepared spermatozoa still motile after 18 h compared with <10% non-processed spermatozoa). Preliminary studies indicate that gradient preparation of spermatozoa may aid survival during cryopreservation.

  1. Caracterización de tres cepas bacterianas capaces de fijar nitrógeno y biodegradar hidrocarburos aisladas de un suelo de la Patagonia

    Directory of Open Access Journals (Sweden)

    A.J. Acuña

    2010-01-01

    Full Text Available El objetivo de este trabajo fue caracterizar tres microorganismos capaces de biodegradar hidrocarburos en ausencia de nitrógeno aislados a partir de un suelo de la Patagonia con una larga historia de contaminación por petróleo y sus derivados. Para ello se aislaron tres cepas bacterianas de un suelo patagónico con baja concentración de nitrógeno y contaminado con hidrocarburos. Estos fueron identificados por ARNr 16S y ácidos grasos de membrana. También se estudiaron sus perfiles de biodegradación de hidrocarburos en presencia y en ausencia de nitrógeno con hidrocarburos alifáticos, cíclicos, aromáticos y poliaromáticos. La capacidad de realizar fijación biológica de nitrógeno se estudió por reducción de acetileno y detección del gen nifD. Por otro lado, se realizó un estudio por desarrollo en cultivo líquido para conocer la adaptación a diferentes pH, entre 5 y 9, temperatura, de 15 a 65 °C, y concentraciones crecientes de NaCl entre 0 y 3M. Los microorganismos aislados correspondieron a los géneros Pseudomonas, Bacillus y Gordonia, todos con capacidad de fijar nitrógeno. Las cepas biodegradaron casi la totalidad de los hidrocarburos ensayados en presencia y ausencia de nitrógeno y tuvieron una buena adaptación a los cambios de pH, temperatura y concentración de NaCl, demostrando una buena adaptación a los principales factores de estrés en los suelos patagónicos.

  2. Sensibilidad y resistencia en el antibiograma del Staphylococcus aureus en pacientes del Hospital Clinico Viedma

    OpenAIRE

    Gustavo Favio Lazo Arispe; Esedim Mamani Flores; Efraín Vargas Loroño; Jhunior Ramiro Camacho Aguilar; Dr. Oscar Sahonero Ampuero

    2013-01-01

    La resistencia bacteriana a los antibióticos es un problema de salud, ya que conlleva la utilización de fármacos más complejos y muy poco accesibles. El objetivo principal de este trabajo es conocer la sensibilidad y resistencia de Staphylococus aureus a los antibióticos, en pacientes que asistieron a Emergencias del Hospital Clínico Viedma. Mediante la realización de un estudio de tipo descriptivo-prospectivo-transversal; se tomó muestras por conveniencia no probabilística de ...

  3. Functional characterisation of semen in honeybee queen (A.m.ligustica S. spermatheca and efficiency of the diluted semen technique in instrumental insemination

    Directory of Open Access Journals (Sweden)

    Andrea Galli

    2010-01-01

    Full Text Available Differences over time in the quality of semen present in the honey bee (Apis mellifera ligustica queen spermatheca werestudied. An increase in the non-vital spermatozoa was shown to be evident (P>0.05 between the 12th and 24th month.The study of semen viability demonstrated that the passage of the semen to the spermatheca is due to sperm motility.In the queen inseminated with non-viable spermatozoa, no semen was detected in the spermatheca. Queens inseminatedtwice with a Hyes solution/semen mixture (1:1 stored as many spermatozoa in their spermatheca as those inseminatedonce with the classic technique. Queen replacement, oviposition and other functional characteristics were similarto those observed in the classic insemination procedure.

  4. Clinical and biochemical correlates of successful semen collection for cryopreservation from 12-18-year-old patients: a single-center study of 86 adolescents

    DEFF Research Database (Denmark)

    Hagenäs, Isabella; Jørgensen, Niels; Rechnitzer, Catherine;

    2010-01-01

    Cryopreservation of semen should be offered to adults before gonadotoxic treatment. However, the experience with semen collection in adolescents is still limited. The objective of this study was to evaluate potential correlates of successful semen sampling in adolescents....

  5. Genetic gain in dairy cattle populations is increased using sexed semen in commercial herds

    DEFF Research Database (Denmark)

    Sørensen, Morten Kargo; Andersen, Jakob Voergaard; Pedersen, Louise Dybdahl

    2011-01-01

    with 100 cows each. Each year 50 young bulls (YB), 10 active sires and 215 BD were selected on best linear unbiased prediction estimated breeding values by truncation selection across the simulated population, and the YB were tested within the population. Use of sexed semen alone gave a positive increase......Using stochastic simulation, the effect of using sexed semen to cow dams (CD) in a dairy cattle breeding scheme, with or without use of multiple ovulation and embryo transfer (MOET) to bull dams (BD), on annual genetic gain at the population level was examined. Three levels of sexed semen were...... combined with three levels of MOET: no sexed semen, sexed semen to the best CD and sexed semen to all heifers, combined with no MOET, MOET on all BD and MOET randomly on 20% of the BD. In total, nine scenarios were compared. The simulated population was monitored for 30 years and included 450 herds...

  6. Identification of Semen Celosiae and Cockscomb Flower Using Continuous Wavelet Transform with FTIR

    Institute of Scientific and Technical Information of China (English)

    Changjiang Zhang; Cungui Cheng

    2006-01-01

    Infrared spectra of semen celosiae and cockscomb flower can be obtained directly, quickly and accurately employing Fourier transform infrared spectroscopy (FTIR) with OMNI sampler. Continuous wavelet transform (CWT) is employed to zoom in local region of infrared spectra of semen celosiae and cockscomb flower. Thus difference of infrared spectra between semen celosiae and cockscomb flower is greatly extruded. Identification rate is greatly improved.Daubechies wavelet is used as mother wavelet. CWT is implemented to the infrared spectra of semen celosiae and cockscomb flower. The difference between semen celosiae and cockscomb flower is observed at all scales in the continuous wavelet domain. An optimal scale is selected to identify semen celosiae and cockscomb flower. Experimental results show that it is effective to apply CWT on the basis of FTIR to identify traditional Chinese medicinal materials, which are the same general but different species.

  7. Influência de diferentes tipos de micro-organismos na contagem bacteriana total por citometria de fluxo do leite cru refrigerado

    Directory of Open Access Journals (Sweden)

    V.S.C. Sampaio

    2015-04-01

    Full Text Available Contagens bacterianas de 179 amostras de leite cru refrigerado, provenientes de diferentes bacias leiteiras de Minas Gerais, foram realizadas pelos métodos de citometria de fluxo e contagem padrão em placas. O estudo das associações entre a contagem bacteriana total e as contagens bacterianas dos diferentes grupos de micro-organismos foi realizado pela correlação de Pearson. Houve uma forte correlação positiva e significativa entre os valores de contagem de psicrotróficos (r = 0,67 e mesófilos (r = 0,70, determinados pelo método de referência, e os valores da contagem bacteriana total, determinados pela citometria de fluxo. Foi possível estabelecer uma curva de calibração para o citômetro de fluxo utilizando equações de regressão linear. As equações para a contagem bacteriana obtida por citometria de fluxo em função da contagem de mesófilos e psicrotróficos foram: log (CBI = 0,701 log mesófilos + 1,9091 e log (CBI = 0,4703 log psicrotróficos + 3,2784, respectivamente. Não foi possível estabelecer curvas de calibrações para termófilos e termodúricos, pois as correlações obtidas entre as contagens desses micro-organismos e a contagem bacteriana total, determinada por citometria de fluxo, foram fracas (r = 0,30 e 0,27, respectivamente.

  8. Trombosis del seno lateral. Complicación de una otitis media subaguda

    OpenAIRE

    Fuente Cañibano, Rebeca de la

    2011-01-01

    [ES] La otitis media es una entidad de gran prevalencia mundial, afectando especialmente a niños menores de 3 años cuando se trata de la forma aguda. La mayor parte de sus complicaciones son de origen infeccioso y para su desarrollo influirán factores como la existencia de bacteriemia, la agresividad del germen causal, la resistencia bacteriana al tratamiento, la correcta instauración del mismo y factores dependientes del individuo como su estado inmunológico. Las complicaciones deben sospech...

  9. Genética y genómica enfocadas en el estudio de la resistencia bacteriana

    OpenAIRE

    Ulises Garza-Ramos; Jesús Silva-Sánchez; Esperanza Martínez-Romero

    2009-01-01

    La resistencia bacteriana es un problema de salud pública causante de índices elevados de morbi-mortalidad hospitalaria. En la medida en que se usan los diferentes antibióticos se seleccionan bacterias resistentes a múltiples fármacos. El desarrollo de nuevas herramientas moleculares de la genómica y proteómica, como el PCR en tiempo real, pirosecuenciación de ADN, espectrometría de masas, microarreglos de ADN y bioinformática, permite conocer en forma más estrecha la fisiología y estructura ...

  10. Avaliação da resistência de clones e cultivares de batata à murcha bacteriana (Ralstonia solanacearum)

    OpenAIRE

    Lima Neto, Artur Ferreira

    2005-01-01

    Cultivares e clones de batata (Solanum tuberosum L.) foram avaliados de 2002 a 2004 na Embrapa Hortaliças, Brasília-DF, para resistência à murcha bacteriana em campo naturalmente infestado com a raça 1, biovar 1 de Ralstonia solanacearum. Os testes de avaliação foram conduzidos com as cultivares atualmente mais plantadas no Brasil e com conjuntos de clones relatados como resistentes no Brasil e em outras partes do mundo. Em todos os experimentos, a doença foi avaliada pela incidência de plant...

  11. Sobre la resistencia bacteriana hacia antibióticos de acción bactericida y bacteriostática

    OpenAIRE

    Jhoana P. Romero L.; Eduardo Ibargüen Mondragón

    2014-01-01

    En este artículo se formula un modelo matemático simple que describe la interacción entre bacterias sensibles y resistentes a múltiples antibióticos de acción bactericida y bacteriostática de forma simultánea, en el supuesto de que la adquisición de resistencia bacteriana se da a través de mutaciones espontáneas y adquiridas por la exposición a diferentes antibióticos. El análisis cualitativo revela la existencia de un equilibrio libre de bacterias, un equilibrio solo con ba...

  12. Estudo prospectivo dos fatores de risco para complicações neurológicas na meningite bacteriana infantil

    Directory of Open Access Journals (Sweden)

    Sadie Namani

    2013-06-01

    Full Text Available OBJETIVO: Análise prospectiva de fatores de prognóstico para complicações neurológicas da meningite bacteriana infantil. MÉTODOS: Este estudo prospectivo recrutou 77 crianças de um mês a 16 anos de idade tratadas de meningite bacteriana durante o período de 1/1/2009 a 31/12/2010. Foram escolhidos 16 preditores relevantes para analisar sua associação com a incidência de complicações neurológicas. Valores P abaixo de 0,05 foram considerados estatisticamente significativos. RESULTADOS: Das 77 crianças tratadas para meningite bacteriana, desenvolveram-se complicações neurológicas em 33 pacientes (43%, e duas crianças morreram (2,6%. A etiologia dos casos de meningite bacteriana foi comprovada em 57/77 (74% dos casos: foram encontrados 32 isolados de meningococos; 8 de pneumococos; 6 de bacilos gram-negativos; 5 de H. influenzae; 5 de estafilococos e 1 de S. viridans. Os fatores que se mostraram associados a aumento do risco de desenvolvimento de complicações neurológicas foram idade 5.000 células/mm³, pleiocitose > 5.000 células/mm³ depois de 48 horas, baixa relação da glicose no LCS/sangue 48 horas, presença de comorbidade e foco primário de infecção não se associaram a aumento do risco para o desenvolvimento de complicações neurológicas. CONCLUSÃO: Idade inferior a 12 meses e gravidade da apresentação clínica na admissão foram identificadas como os preditores mais fortes de complicações neurológicas e podem ter valor para selecionar pacientes para tratamento mais intensivo.

  13. Effect of different antioxidant additives in semen diluent on cryopreservability (−196°C of buffalo semen

    Directory of Open Access Journals (Sweden)

    Hardik A. Patel

    2016-03-01

    Full Text Available Aim: The aim of this study was to evaluate the effect of different antioxidant additives in standard tris-fructose-egg yolkglycerol (TFYG extender on the cryopreservability of buffalo semen. Materials and Methods: Semen collection using artificial vagina, twice weekly for 5 weeks from three pedigreed health breeding bulls of Mehsani breed, aged between 6 and 8 years. Immediately after initial evaluation all 30 qualifying ejaculates (10/bull were split into three aliquots and diluted at 34°C keeping the concentration of 100 million spermatozoa/ml with standard TFYG extender as control and TFYG having two antioxidant additives - Cysteine HCl at 1 mg/ml and ascorbic acid at 0.2 mg/ml to study their comparative performance. Semen filled in French Mini straws using IS-4 system and gradually cooled to 4°C and equilibrated for 4 h in cold handing cabinet. After completion of equilibration, straws were cryopreserved in LN2 by Programmable Bio-freezer. Semen was examined at post-dilution, post-equilibration, and post-thaw stages for sperm quality parameters, and at each stage plasma was separated for enzymatic analysis of aspartate aminotransferase (AST, lactate dehydrogenase (LDH, and alkaline phosphatase (AKP. Results: The mean percentage of sperms in TFYG, TFYG + cysteine HCl and TFYG + ascorbic acid diluents at postthaw stage in terms of progressive motility (52.83±0.52, 57.83±0.52, 57.83±0.52, livability (78.70±0.21, 82.33±0.23, 81.73±0.22, and abnormality (5.43±0.21, 5.03±0.17, 5.23±0.18 varied significantly (p<0.05 between control TFYG and TFYG having antioxidant additives. The mean U/L activities of AST (78.70±0.47, 72.80±0.48, 73.30±0.54, LDH (172.70±0.41, 155.78±0.42, 156.33±0.41, and AKP (103.61±0.34, 90.20±0.34, 91.03±0.34 in semen diluted with TFYG, TFYG + cysteine HCl and TFYG + ascorbic acid diluents at post-thaw stage, respectively, which showed significantly (p<0.05 higher leakage of enzymes in control TFYG than TFYG

  14. Bull breeding soundness, semen evaluation and cattle productivity.

    Science.gov (United States)

    Chenoweth, P J; McPherson, F J

    2016-06-01

    The bull breeding soundness evaluation (BBSE) has evolved as a cost-effective veterinary procedure which provides benefits such as risk-reduction and improvements in strategic bull usage, herd fertility and economics. Semen evaluation is an important component of the BBSE when performed appropriately; a consideration that is increasingly addressed by third party andrology laboratories. The combination of competent physical/reproductive exams (including scrotal circumference measurements) and semen evaluations can contribute greatly to the fertility and economics of individual herds as well as adding to understanding of those factors which affect cattle fertility. Despite such advantages, there remain challenges in achieving full acceptance of BBSEs, particularly by the dairy industry and in developing countries.

  15. Semen collection and evaluation of captive coatis (Nasua nasua

    Directory of Open Access Journals (Sweden)

    R.C.R. Paz

    2012-04-01

    Full Text Available Semen samples (n=105 were collected through eletroejaculation from six adult male coatis (Nasua nasua between January 2007 and December 2008 at Universidade Federal de Mato Grosso Zoo, Cuiabá, Brazil. Mean values were: volume (mL; concentration (sperm/mL; total motility (%; progressive sperm motility (scale, 0-5; live spermatozoa (%; acrossome integrity (%; primary defects (%; and secondary defects (%. There was high correlation between total motility and live sperm; total motility and progressive sperm motility; total motility and acrossome integrity; live sperm and progressive motility; live sperm and acrossome integrity and volume and concentration. The method for semen collection was considered safe and efficient. It can be used for the evaluation of breeding potential of coati in captivity and for the establishment of new assisted reproductive technology (ART for threatened neotropical carnivores species.

  16. Parental age at delivery and a man's semen quality

    DEFF Research Database (Denmark)

    Priskorn, Lærke; Jensen, Tina K; Lindahl-Jacobsen, Rune;

    2014-01-01

    the possibility that a trend may have been identified with a still larger sample. In addition, the Danish Civil Registration System is merely administrative and hence does not discriminate between biological and adopted children. However, the low rate of adoption (≈2%) suggests that misclassification would have......STUDY QUESTION: Is parental age at delivery associated with a man's semen quality? SUMMARY ANSWER: In this large register-based study both mother's and father's age are found to have minimal effects on semen quality in men. WHAT IS KNOWN ALREADY: Both maternal and paternal age have been associated...... with a range of adverse health effects in the offspring. Given the varied health effects of parental age upon offspring, and the sensitivity of genital development to external factors, it is plausible that the age of a man's mother and father at conception may impact his reproductive health. To our knowledge...

  17. Identification and determination of (+)-sesamin in Semen Cuscutae by capillary GC and GC-MS

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    (+)-Sesamin was found in Semen Cuscutae for the first time. A rapid and simple approach for the analysis of (+)-sesamin in different sources of Semen Cuscutae is proposed, which used GC-FID for the determination of (+)-sesamin and GC-MS for its identification. The result suggested that this approach could be used to identify Semen Cuscutae from various sources based on the different content of (+)-sesamin in them.

  18. Gallic Acid Is an Antagonist of Semen Amyloid Fibrils That Enhance HIV-1 Infection.

    Science.gov (United States)

    LoRicco, Josephine G; Xu, Changmingzi Sherry; Neidleman, Jason; Bergkvist, Magnus; Greene, Warner C; Roan, Nadia R; Makhatadze, George I

    2016-07-01

    Recent in vitro studies have demonstrated that amyloid fibrils found in semen from healthy and HIV-infected men, as well as semen itself, can markedly enhance HIV infection rates. Semen fibrils are made up of multiple naturally occurring peptide fragments derived from semen. The best characterized of these fibrils are SEVI (semen-derived enhancer of viral infection), made up of residues 248-286 of prostatic acidic phosphatase, and the SEM1 fibrils, made up of residues 86-107 of semenogelin 1. A small molecule screen for antagonists of semen fibrils identified four compounds that lowered semen-mediated enhancement of HIV-1 infectivity. One of the four, gallic acid, was previously reported to antagonize other amyloids and to exert anti-inflammatory effects. To better understand the mechanism by which gallic acid modifies the properties of semen amyloids, we performed biophysical measurements (atomic force microscopy, electron microscopy, confocal microscopy, thioflavin T and Congo Red fluorescence assays, zeta potential measurements) and quantitative assays on the effects of gallic acid on semen-mediated enhancement of HIV infection and inflammation. Our results demonstrate that gallic acid binds to both SEVI and SEM1 fibrils and modifies their surface electrostatics to render them less cationic. In addition, gallic acid decreased semen-mediated enhancement of HIV infection but did not decrease the inflammatory response induced by semen. Together, these observations identify gallic acid as a non-polyanionic compound that inhibits semen-mediated enhancement of HIV infection and suggest the potential utility of incorporating gallic acid into a multicomponent microbicide targeting both the HIV virus and host components that promote viral infection.

  19. Embryo production with sex-sorted semen in superovulated dairy heifers and cows.

    Science.gov (United States)

    Kaimio, I; Mikkola, M; Lindeberg, H; Heikkinen, J; Hasler, J F; Taponen, J

    2013-11-01

    The aim of this study was to examine the effect of sex-sorted semen on the number and quality of embryos recovered from superovulated heifers and cows on commercial dairy farm conditions in Finland. The data consist of 1487 commercial embryo collections performed on 633 and 854 animals of Holstein and Finnish Ayrshire breeds, respectively. Superovulation was induced by eight intramuscular injections of follicle-stimulating hormone, at 12-hour intervals over 4 days, involving declining doses beginning on 9 to 12 days after the onset of standing estrus. The donors were inseminated at 9 to 15-hour intervals beginning 12 hours after the onset of estrus with 2 + 2 (+1) doses of sex-sorted frozen-thawed semen (N = 218) into the uterine horns or with 1 + 1 (+1) doses of conventional frozen-thawed semen (N = 1269) into the uterine corpus. Most conventional semen (222 bulls) straws contained 15 million sperm (total number 30-45 million per donor). Sex-sorted semen (61 bulls) straws contained 2 million sperm (total number 8-14 million per donor). Mean number of transferable embryos in recoveries from cows bred with sex-sorted semen was 4.9, which is significantly lower than 9.1 transferable embryos recovered when using conventional semen (P ≤ 0.001). In heifers, no significant difference was detected between mean number of transferable embryos in recoveries using sex-sorted semen and conventional semen (6.1 and 7.2, respectively). The number of unfertilized ova was higher when using sex-sorted semen than when using conventional semen in heifers (P cows (P cows (P protocol used seemed to be adequate for heifers. In superovulated cows, an optimal protocol for using sex-sorted semen remains to be found.

  20. ANALISIS ECONOMIC VALUE ADDED UNTUK MENILAI KINERJA KEUANGAN PERUSAHAAN SEMEN YANG TERDAFTAR DI BURSA EFEK INDONESIA

    OpenAIRE

    PATA, MARIANUS

    2015-01-01

    2015 Tujuan dari penelitian ini adalah untuk mengetahui kinerja keuangan pada perusahaan semen yang terdaftar di Bursa Efek Indonesia melalui metode analisis Economic Value Added sebagai alat ukur kinerja keuangan pada perusahaan semen yang terdaftar di Bursa Efek Indonesia. Data penelitian ini diperoleh dari laporan keuangan tahun 2011-2013 masing-masing perusahaan yang terdaftar di Bursa Efek Indonesia. Hasil analisis kinerja keuangan dari 5 perusahaan semen yang ter...

  1. Controle biológico da murcha bacteriana do tomateiro, por Pseudomonas spp. fluorescentes Biological control of bacterial wilt of tomato by fluorescent Pseudomonas spp

    OpenAIRE

    Ana Rosa Peixoto

    1997-01-01

    Esta revisão bibliográfica teve como objetivo avaliar o potencial de antagonismo de espécies de Pseudomonas fluorescentes a Pseudomonas solanacearum, agente causal da murcha bacteriana do tomateiro. Devido a dificuldade encontrada nas estratégias utilizadas para o controle da Murcha Bacteriana por meio de métodos convencionais, alguns outros tem sido estudados, como o uso de microrganismos benéficos. As rizobactérias vem proporcionando solução viável a algumas doenças consideradas de difícil ...

  2. O uso de antibióticos e as resistências bacterianas: breves notas sobre a sua evoluc¸ão

    OpenAIRE

    Loureiro, Rui João; Roque, Fátima; Teixeira-Rodrigues, António; Herdeiro, Maria Teresa; Ramalheirae, Elmano

    2016-01-01

    A resistência bacteriana aos antibióticos é atualmente um dos problemas de saúde pública mais relevantes a nível global, dado que apresenta consequências clínicas e económicas preocupantes, estando associada ao uso inadequado de antibióticos. Portugal é, no contexto europeu, um país com um elevado consumo de antibióticos, apesar de uma diminuic¸ão no consumo destes fármacos nos últimos anos. A resistência bacteriana tem crescido acentuadamente, sendo que as bactérias Gram-posit...

  3. Etiologia e evolução das meningites bacterianas em centro de pediatria Etiology and evolution of bacterial meningitis in a pediatric center

    OpenAIRE

    Roberta M.C. Romanelli; Claudete A. Araújo; Fernando Boucinhas; Carvalho,Inácio R.; Nelson R.L. Martins; Heliane B. M. Freire

    2002-01-01

    Objetivo: determinar a prevalência dos agentes etiológicos das meningites bacterianas em serviço de referência, no atendimento de doenças infecciosas para o estado de Minas Gerais, e verificar a resposta ao tratamento utilizado.Métodos: estudo descritivo em que foram incluídas todas as crianças com diagnóstico provável de meningite, admitidas na instituição no período de junho a novembro/99.Resultados: obteve-se 210 casos de meningite, sendo 111 casos de etiologia bacteriana (52,9%). Destes, ...

  4. A orbifloxacina no tratamento das cistites bacterianas em gatos domésticos The orbifloxacin in the treatment of lower urinary tract infections in cats

    OpenAIRE

    Archivaldo Reche Junior

    2005-01-01

    O presente estudo teve como objetivos, avaliar o uso da orbifloxacina nas cistites bacterianas de felinos, e seus possíveis efeitos colaterais, mormente a nefro e/ou hepatotoxicidade. Para tanto, foram colhidas amostras de urina de 53 gatos com históricos variados, mas com predisposição à infecção urinária. A cistite bacteriana foi comprovada, através de exame microbiológico, em 13 pacientes. No entanto, apenas 10 apresentaram infecção por agentes sensíveis a orbifloxacina. Esses 10 pacientes...

  5. Strategies to improve the fertility of fresh and frozen donkey semen.

    Science.gov (United States)

    de Oliveira, José Victor; Oliveira, Pedro Victor de Luna Freire; Melo e Oña, Cely Marini; Guasti, Priscilla Nascimento; Monteiro, Gabriel Augusto; Sancler da Silva, Yamê Fabres Robaina; Papa, Patrícia de Mello; Alvarenga, Marco Antônio; Dell'Aqua Junior, Jose Antonio; Papa, Frederico Ozanam

    2016-04-15

    Fertility rates of donkey semen in jennies are lower compared to mares. The aims of this study were to evaluate different sperm cryopreservation methods and insemination strategies to improve the fertility of donkey semen in jennies. Three experiments were performed: (1) the comparison of two freezing methods of donkey semen (conventional method and automated method); (2) the determination of a suitable insemination dose of fresh donkey semen for jennies and mares; and (3) the influence of the semen deposition site on fertility of jennies inseminated with frozen donkey semen. For experiment 1, no differences were observed in total motility, angular velocity, curvilinear velocity, straight-line velocity, and plasma membrane integrity between samples frozen with the conventional (Styrofoam box) and the automated method (TK 4000C). However, the automated method provided higher values of progressive motility and rapid cells in frozen-thawed samples in comparison with the conventional method (P frozen-thawed donkey semen. No pregnancies were achieved with inseminations performed in the UB (0/12). The pregnancy rate for uterine horn group was 28.26% (13/46) and thus significantly higher than the UB group (0%; 0/12; P frozen-thawed donkey semen increased the pregnancy rate in jennies, and the multiple inseminations may be an option to improve the fertility rates of donkey semen in jennies.

  6. Cadmium may impair prostate function as measured by prostate specific antigen in semen

    DEFF Research Database (Denmark)

    Andreucci, Alessandro; Mocevic, Emina; Jönsson, Bo A G

    2015-01-01

    We investigated the association between cadmium in blood and the concentration of the prostate specific antigen (PSA) in semen, including the modifying effects of zinc or the CAG polymorphism in the androgen receptor (AR). Blood and semen samples were collected from 504 partners of pregnant women.......0009). Inverse trends between cadmium and PSA were found when semen zinc concentrations were below the median value for men from Ukraine and Greenland. These outcomes suggest that cadmium may impair prostate function, as measured by PSA in semen, while high zinc levels and a low number of CAG repeats protects...

  7. Pengaruh Berbagai Konsentrasi Dimethylsulfoxide terhadap Kualitas Semen Beku Ayam Hutan Hijau Post Thawing

    Directory of Open Access Journals (Sweden)

    Wayan Bebas

    2015-06-01

    Full Text Available The process of freezing and thawing on semen can lead to physical stress, often called cold shock, and couses the structural and biochemical damage that affecting cell function and ultimately lead to the death of the cell The aim of this study was to know the effect of the addition of various concentrations of dimethylsulfoxide (DMSO as the intracellular cryoprotectant in phosphate yolk diluent on the post thowing quality of the green jungle fowl semen. The study used eight green jungle fowl semens which were collected with massage techniques. Semen was evaluated macroscopically and microscopically. Good quality semen was diluted with phosphate yolk which was added four different concentration of DMSO, namely 4%, 6%, 8%, and 10%. Semen was then filled and sealed in a mini straw (0.25 mL with the concentration of 150.106 cells, and equilibrated at 4oC for 4 hours. The semen freezing was processed using conventional method. Evaluation was performed on post thawing semen. The evaluation of semen quality included the progressive motility and plasma membrane intact. Data were analyzed by analysis of variance. If there were any significant differences, the data were futher analyzed by Duncan test. The results showed that addition of DMSO concentration of 6% has resulted the progressive motility and intact plasma membrane higher significantly (P <0.05 than those of the addition of DMSO concentration 4%, 8%, and 10%.

  8. PROSTAGLANDIN F2α SUPPLEMENTED SEMEN IMPROVES LANDRACE BOARS SPERM MOTILITY

    Directory of Open Access Journals (Sweden)

    IOANA SGURĂ

    2013-07-01

    Full Text Available This study investigated whether the sperm motility from Landrace boars improves when PGF2α (Dinolytic®; 5 mg PGF2α /ml was added to diluted semen. Boars from one large production unit, were manually collected; semen was either enriched with PGF2α (group 1, n=38, either untreated (group 2, n=32. Total volume of semen collected, percent of motility and number of obtained doses were recorded. The highest sperm volume collected from the two groups is corresponding to ejaculates from Landrace boars with PGF2α supplemented semen (267.6 ml. Regarding motility, the sperm collected from Landrace boars with PGF2α supplemented semen was higher from the one collected from Landrace boars with untreated semen (81.37% and very significant differences were statistically determined. The ejaculates with highest number of obtained doses is corresponding to the ones collected from boars with PGF2α supplemented semen (25.21. Only boars from the first group (with PGF2α supplemented semen showed motility over 70% and even 100%. The untreated semen showed motility values around 65-70%.

  9. Semen quality in Schistosoma haematobium infected men in Madagascar.

    Science.gov (United States)

    Leutscher, Peter D C; Høst, Erik; Reimert, Claus M

    2009-01-01

    The seminal vesicles and the prostate are frequently affected by egg-induced inflammation in Schistosoma haematobium infected men. The objective of this study was to assess the semen quality in men with male genital schistosomiasis (MGS). The examination of the semen samples was performed in men aged 15 to 49 years living an S. haematobium endemic area in Madagascar prior to anti-schistosoma treatment with praziquantel and five months later. Men from the high endemic Sirama sugarcane plantation with positive egg excretion in the urine and circulating anodic antigen (CAA) present in serum (n=85) were compared to men (without egg excretion and no CAA) from the neighbouring low-endemic Mataipako village (n=57). The proportion of men with egg excretion in semen was significantly higher in Sirama than in Mataipako (53% versus 4%), whereas the median ejaculate volume was significantly lower in Sirama (1.8 ml versus 2.4 ml). There was no statistical difference in median spermatocyte counts and in the proportions of men detected with azoospermia. The mean apoptotic rate was 7.8% in a subgroup of 30 men from Sirama. A positive correlation was found between apoptotic rate and seminal eosinophil cationic protein (ECP) level (rho=0.560; Pinfection is associated with sperm apoptosis and a reduced production of seminal fluid. Egg induced inflammation in the seminal vesicles and the prostate could be underlying mechanism for both observations.

  10. Prospective surveillance of semen quality in the workplace

    Energy Technology Data Exchange (ETDEWEB)

    Schenker, M.B.; Samuels, S.J.; Perkins, C.; Lewis, E.L.; Katz, D.F.; Overstreet, J.W.

    1988-04-01

    We performed a prospective surveillance of semen quality among workers in the plant where 1,2-dibromo-3-chloropropane was first recognized as an occupational cause of impaired semen quality and of infertility. All male employees of the Agricultural Chemical Division were required to participate. Ninety-seven workers (92% participation) provided 258 semen samples over the 4 years of the program. Most samples were analyzed at the plant with a mini-laboratory designed for the study. Motility and shape measures were made objectively. Sixty-six subjects (68%) were non-azoospermic. Generalized multiple regression showed no significant predictors for any response, with the exception of the motility measures, which were reduced with longer times between ejaculation and assay. Between- and within-person standard deviations and correlations were calculated. Comparison of this population with fertile artificial insemination donors (16 men, 498 ejaculates) revealed generally higher ejaculate-to-ejaculate standard deviations in the worker samples. This is probably due to less well controlled conditions of sperm collection in the workplace setting. For cross-sectional studies, one ejaculate per worker is recommended as sufficient; for estimating an individual worker's mean, even three ejaculates may not provide enough precision.

  11. Glyph-Based Video Visualization for Semen Analysis

    KAUST Repository

    Duffy, Brian

    2015-08-01

    © 2013 IEEE. The existing efforts in computer assisted semen analysis have been focused on high speed imaging and automated image analysis of sperm motility. This results in a large amount of data, and it is extremely challenging for both clinical scientists and researchers to interpret, compare and correlate the multidimensional and time-varying measurements captured from video data. In this work, we use glyphs to encode a collection of numerical measurements taken at a regular interval and to summarize spatio-temporal motion characteristics using static visual representations. The design of the glyphs addresses the needs for (a) encoding some 20 variables using separable visual channels, (b) supporting scientific observation of the interrelationships between different measurements and comparison between different sperm cells and their flagella, and (c) facilitating the learning of the encoding scheme by making use of appropriate visual abstractions and metaphors. As a case study, we focus this work on video visualization for computer-aided semen analysis, which has a broad impact on both biological sciences and medical healthcare. We demonstrate that glyph-based visualization can serve as a means of external memorization of video data as well as an overview of a large set of spatiotemporal measurements. It enables domain scientists to make scientific observation in a cost-effective manner by reducing the burden of viewing videos repeatedly, while providing them with a new visual representation for conveying semen statistics.

  12. Antioxidant supplements and semen parameters: An evidence based review

    Science.gov (United States)

    Ahmadi, Sedigheh; Bashiri, Reihane; Ghadiri-Anari, Akram; Nadjarzadeh, Azadeh

    2016-01-01

    Many studies have focused on male infertility. There is limited evidence about the influence of nutrition on quality of semen. Approximately, 30-80% of infertility cases are caused by oxidative stress and decreased level of seminal total antioxidant capacity. This study was aimed to review the effects of oral antioxidant supplements on improving major semen parameters such as sperm concentration, motility, morphology, DNA damage, and fertility rate. Data were extracted from PubMed and Google scholar database by using the terms “antioxidant”, “multivitamin”, “carnitine”, “CoQ10”, “vitamin C”, “vitamin E”, “zinc”, “folic acid”, “N-acetyl cysteine” and “selenium” combined with “male infertility”, “semen”, and “sperm” to generate a set of relevant citations. Supplements such as CoQ10 and alpha-tocopherol significantly improve sperm count. Also, carnitine has positive effects on sperm motility and morphology. Simultaneous administration of vitamin E and vitamin C reduces the sperm DNA damage. However, in some studies, one or more factors have not changed substantially. In most of the studies, antioxidant supplementation improved the number, motility, morphology and sometimes DNA integrity of sperm. The present study showed that antioxidant supplements, especially a combination of antioxidants such as vitamin C, vitamin E, and CoQ10 intake can effectively improve semen parameters in infertile men. PMID:28066832

  13. Fennel (Foeniculum vulgare) provides antioxidant protection for boar semen cryopreservation.

    Science.gov (United States)

    Malo, C; Gil, L; Cano, R; González, N; Luño, V

    2012-05-01

    Boar semen is extremely vulnerable to cold shock and it is also sensitive to peroxidation due to the high content of unsaturated fatty acids in the plasma membrane. Antioxidants exert a protective effect on the plasma membrane of frozen boar sperm. Fennel has been shown to contain antioxidant substances. Therefore, this study was performed to evaluate the effect of different concentrations of fennel added to the freezing extender on boar semen quality and lipid peroxidation after thawing. Semen collected from four boars was cryopreserved in lactose-egg-yolk extender or in the same extender with varying concentration of fennel essences: low (LF); medium (MF); high (HF). Analysis of data clearly indicated that higher concentrations of fennel produced significant improvement in total motility. Moreover, when fennel was included in the extender, a dose-dependent tendency to increase sperm viability was observed. In contrast, the addition of fennel had no effect on acrosome integrity or hypoosmotic swelling test (HOST) compared with the control. Malondialdehyde (MDA) formation decreased significantly in fennel groups, yielding similar results for MF and HF. Fennel seems a new antioxidant for use in sperm cryopreservation, but its particular effects on sperm physiology must be further studied, especially the causes of motility stimulation and its effect on lipoxidation.

  14. Sperm banking for male cancer patients: social and semen profiles

    Directory of Open Access Journals (Sweden)

    Tatiana C.S. Bonetti

    2009-04-01

    Full Text Available PURPOSE: Report the characteristics of cryopreserved semen from a cohort of male cancer patients, attitudes towards cryopreservation and outcomes of semen samples based on a 12-year cryopreservation program. MATERIAL AND METHODS: Data from 98 male cancer patients whose sperm samples were banked were evaluated. Demographic parameters, semen characteristics, destination of sperm banked samples and questionnaires answered by the patients regarding cryopreservation time were evaluated. RESULTS: The cancer diagnoses were testicle (56.1%, prostate (15.3%, Hodgkin’s lymphomas (9.2%, non-Hodgkin’s lymphomas (7.1%, leukemia (3.1% and other malignancies (9.2%. The patients with testicular cancer presented lower sperm concentration (p < 0.001; however, there were no differences with the percentage of normozoospermic patients among cancer type groups (p = 0.185. A shorter time between cancer diagnosis and sperm banking was observed for testicular and prostate cancer patients (p < 0.001. Most of the patients (89.5% favored sperm banking as a fertility preservation method. CONCLUSIONS: Although less than 20% of banked sperm samples were disposed of, the majority of patients related sperm banking with safe for fertility preservation. Our results show that all male cancer patients of reproductive age facing cancer treatment could be offered sperm banking.

  15. Semen analysis standardization: is there any problem in Polish laboratories?

    Science.gov (United States)

    Walczak-Jedrzejowska, Renata; Marchlewska, Katarzyna; Oszukowska, Elzbieta; Filipiak, Eliza; Bergier, Leszek; Slowikowska-Hilczer, Jolanta

    2013-09-01

    The aim of the study was to determine the degree of compliance of Polish laboratories with World Health Organization (WHO) recommendations, with regard to semen analysis methodology. A survey requesting information about methods of semen analysis was distributed to employees of 55 laboratories. Respondents who had participated in external seminological workshops (31%) were termed certified respondents (CR), the remaining (69%)-non-certified respondents (NCR). Only one laboratory (6%) in the CR group and none in the NCR were compliant with WHO guidelines for methods and equipment used to evaluate seminal volume, sperm motility, concentration, vitality and morphology. Most problems were of volume measurement (weighing method was reported by 17% of CR and 10% of NCR) and staining method for sperm morphology (Papanicolau or Diff-Quik were found in 33% of CR and 23% of NCR). A three- or four-point grading of sperm motility was used by the majority of respondents; however, 17% of CR and 37% of NCR did not use a laboratory counter to tally spermatozoa. Although a haemocytometer method was used by 80% of laboratories in each group, the improved Neubauer chamber was used only by 42% of CR and 19% of NCR. In each group, 24% of laboratories did not perform a vitality test. Procedural errors and the interchangeable utilization of two or even three methods to analyse a given parameter was observed in both groups. The results indicate a need for standardisation of the methods and continuous, unified training in semen analysis in Polish laboratories.

  16. Decreases in Human Semen Quality with Age Among Healthy Men

    Energy Technology Data Exchange (ETDEWEB)

    Eskenazi, B.; Wyrobek, A.J.; Kidd, S.A.; Moore, L.; Young, S.S.; Moore, D.

    2001-12-01

    The objective of this report is to characterize the associations between age and semen quality among healthy active men after controlling for identified covariates. Ninety-seven healthy, nonsmoking men between 22 and 80 years without known fertility problems who worked for or retired from a large research laboratory. There was a gradual decrease in all semen parameters from 22-80 years of age. After adjusting for covariates, volume decreased 0.03 ml per year (p = 0.001); sperm concentration decreased 2.5% per year (p = 0.005); total count decreased 3.6% per year of age (p < 0.001); motility decreased 0.7% per year (P < 0.001); progressive motility decreased 3.1% per year (p < 0.001); and total progressively motile sperm decreased 4.8% per year (p < 0.001). In a group of healthy active men, semen volume, sperm concentration, total sperm count, and sperm motility decrease continuously between 22-80 years of age, with no evidence of a threshold.

  17. Semen analysis in chronic bacterial prostatitis: diagnostic and therapeutic implications

    Science.gov (United States)

    Magri, Vittorio; Wagenlehner, Florian M E; Montanari, Emanuele; Marras, Emanuela; Orlandi, Viviana; Restelli, Antonella; Torresani, Erminio; Naber, Kurt G; Perletti, Gianpaolo

    2009-01-01

    The significance and diagnostic value of semen analysis in chronic bacterial prostatitis has been extensively debated and remains controversial. To investigate the diagnostic relevance of semen culture in the bacteriological workup of prostatitis patients, we retrospectively analyzed a clinical database of 696 symptomatic patients. All patients were routinely subjected to a four-glass test, followed by semen culture and analysis. This allowed to dissect from the database three different diagnostic scenarios, and to compare the 'two-glass' pre-/post- massage test and the standard 'four-glass' test with a 'five-glass' test (four-glass plus post-VB3 semen culture). The 'five-glass' test showed 3.6- or 6.5-fold increases in relative sensitivity and lesser reductions (−13.2% or −14.7%) in relative specificity for traditional uropathogens (TUs) compared with the four-glass or two-glass test, respectively. The area under the ROC curve and Jouden's index were increased, whereas positive and negative likelihood ratios were lower than comparators, indicating that the 'five-glass' assay may be superior in confirming the negative outcome of both standard tests. The five-, four-, and two-glass tests detected TUs (Enterobacteriaceae, Enterococci, etc.) in 120, 33, and 20 patients and unusual pathogens (Streptococci, other Gram-positive species, Mycoplasmata, and others) in 130, 56, and 45 patients, respectively. When patients were subjected to pharmacological treatment, including a combination of a fluoroquinolone and a macrolide, no differences in eradication rates were observed between groups diagnosed with different tests, irrespective of pathogen category. Eradication was associated with long-term sign/symptom remission; no significant intergroup differences in sign/symptom scores were observed throughout a 24-month off-therapy follow-up period. In conclusion, our data support the usefulness of semen analysis in the diagnostic workup of prostatitis patients when this

  18. Peritonitis bacteriana espontánea y bacteriemia por Aeromonas hydrophila Spontaneous bacterial peritonitis and bacteraemia due to Aeromonas hydrophila

    Directory of Open Access Journals (Sweden)

    J. J. García-Irure

    2003-12-01

    Full Text Available La peritonitis bacteriana es una complicación frecuente en los pacientes cirróticos. De los gérmenes etiológicos, Aeromonas es muy poco frecuente. Presentamos un caso clínico de un paciente cirrótico con peritonitis y bacteriemia por Aeromonas hydrophila. Este microorganismo es un bacilo gramnegativo de la familia Vibrionaceae. En el ser humano produce gastroenteritis con mucha frecuencia. De forma excepcional puede dar origen a infecciones extraintestinales, sobre todo en pacientes inmunodeprimidos. La peritonitis por Aeromonas se ha descrito en asociación a peritonitis bacteriana espontánea en pacientes cirróticos, en pacientes en diálisis peritoneal y en casos de peritonitis secundaria a perforación intestinal.Bacterial peritonitis is a frequent complication in cirrhotic patients. Amongst the aetiological germs, Aeromonas is very infrequent. We present the clinical case of a cirrhotic patient with peritonitis and bacteraemia due to Aeromonas hydrophila. This micro-organism is a Gram-negative bacillus of the Vibrionaceae family. It very frequently produces gastroenteritis in the human being. Exceptionally it can give rise to extraintestinal infections, especially in immunodepressed patients. Peritonitis due to Aeromonas has been described in association with spontaneous bacterial peritonitis in cirrhotic patients, in patients on peritoneal dialysis and in cases of peritonitis secondary to intestinal perforation.

  19. Fatores de risco para meningite bacteriana no recém-nascido Risk factors for bacterial meningitis in the newborn

    Directory of Open Access Journals (Sweden)

    Vera Lúcia Jornada Krebs

    2004-09-01

    Full Text Available O objetivo do estudo é descrever os fatores de risco para meningite bacteriana em recém-nascidos e analisar a prevalência destes fatores, considerando-se a presença ou não de baixo peso ao nascimento. Foram analisados 50 recém-nascidos com meningite bacteriana, excluindo-se aqueles com meningomielocele ou infecção congênita. Na análise estatística utilizou-se o teste exato de Fisher, considerando-se significantes os valores de p The aim of this study is to describe the risk factors for bacterial meningitis in newborns, and to analyze the prevalence of these factors, considering or not the low birth weight presence. Fifty newborns with bacterial meningitis were analyzed, excluding the ones with meningomyelocele or congenital infection. In the statistical analysis, the Fisher's exact test was used, considering significant the p < 0.05 values. This study has shown that prematurity, low birth weight and presence of previous infectious diseases in the newborn or in the mother were important risk factors for meningitis. Among low birth weight newborns, invasive procedures, especially tracheal intubation, use of central venous catheter and previous use of antibiotics, were significantly associated to the meningitis occurrence. These results indicate that the improvement in the prenatal care and in the hospital infection control are measures of high importance in the decrease of the incidence of neonatal bacterial meningitis.

  20. Survey of carnitine content of human semen using a semiquantitative auxanographic method: decreased semen total carnitine concentration in patients with azoospermia or severe oligozoospermia.

    Science.gov (United States)

    Soffer, Y; Shalev, D P; Weissenberg, R; Orenstein, H; Nebel, L; Lewin, L M

    1981-01-01

    A microbiological method, using the carnitine-requiring yeast, Torulopsis bovina ATCC 26014, was developed to identify samples of human semen which contained low levels (less than 250 micron M) of total carnitine. Of 399 semen samples from a male infertility clinic which were tested, 30 (7.5%) were low in carnitine. Of these, 14 were azoospermic and 16 were severely oligozoospermic. Some azoospermic samples (19 = 58%) and severely oligozoospermic samples (51 = 79%) did not give evidence of low carnitine concentrations. These results indicate that decreased total carnitine concentration in semen occurs in certain classes of azoospermic and severely oligozoospermic patients.

  1. Body mass index in relation to semen quality and reproductive hormones among 1,558 Danish men

    DEFF Research Database (Denmark)

    Jensen, Tina Kold; Andersson, Anne-Maria; Jørgensen, Niels;

    2004-01-01

    To examine the relationship between body mass index (BMI) and semen quality among young men from the general population.......To examine the relationship between body mass index (BMI) and semen quality among young men from the general population....

  2. An effective method for improving the fertility of glycerol-exposed poultry semen.

    Science.gov (United States)

    Long, J A; Kulkarni, G

    2004-09-01

    Semen cryopreservation is necessary for banking germplasm from critical poultry stocks. To date, glycerol is the most effective cryoprotectant for poultry sperm; however, the contraceptive effects of glycerol require a significant reduction of the cryoprotectant from thawed semen before artificial insemination (AI). The effectiveness of glycerol reduction by dialysis, Percoll density gradient centrifugation, or washing through 12% (wt/vol) Accudenz was evaluated by fertility trials with highly inbred chicken research lines and commercial turkey lines. Semen was extended 1:1 and then diluted with glycerolized extender to yield a final 11% (vol/vol) glycerol concentration. Glycerolized rooster semen was aliquoted for control, Accudenz centrifugation, and dialysis treatments. A total of 90 pure line and 85 F1 hybrid chicken hens were each inseminated with 100 x 10(6) sperm at 7-d intervals for 4 to 6 wk. All eggs from the glycerolized control semen treatments were infertile, and fertility rates from dialyzed semen decreased steadily from 26.4 to 0% within the first 4 wk for the pure lines. In contrast, fertility rates for Accudenz-processed semen increased from 17.9 to 37.17% during the first 4 wk. Similar fertility rates occurred with the F1 hybrid cross lines. For turkey fertility trials, the dialysis treatment was not used; glycerolized turkey semen was processed by Accudenz or Percoll centrifugation to reduce glycerol. A total of 36 hens were inseminated with 150 x 10(6) sperm at 7-d intervals for 6 wk. Similar to the chicken trials, fertility rates of Accudenz-processed semen steadily increased to 49.4% by the sixth week of insemination. The average fertility of Percoll-processed semen was only 19.1%. These data demonstrate that Accudenz centrifugation is an acceptable glycerol reduction method for nonfrozen poultry semen.

  3. Effect of species, breed, and age on bacterial load in bovine and bubaline semen

    Directory of Open Access Journals (Sweden)

    Chandrahas Sannat

    2015-04-01

    Full Text Available Aim: The present study was conducted to investigate the effect of species, breed and age on bacterial load in fresh and frozen semen of Cattle and Buffalo bull. Materials and Methods: Present study covered 56 cow and 10 buffalo bulls stationed at Central Semen Station Anjora, Durg (Chhattisgarh. Impact of breeds on bacterial load in semen was assessed using six breeds of cattle viz. Sahiwal, Gir, Red Sindhi, Tharparkar, Jersey and Holstein Friesian (HF cross. Cow bulls were categorized into four different groups based on their age ( 6 years to study variation among age groups. Bacterial load was measured in fresh and frozen semen samples from these bulls using the standard plate count (SPC method and count was expressed as colony forming unit (CFU per ml of semen. Results: Higher bacterial load was reported in fresh (2.36 × 104 ± 1943 CFU/ml and frozen (1.00 × 10 ± 90 CFU/ml semen of cow bulls as compared to buffalo bulls (1.95 × 104 ± 2882 and 7.75 × 102 ± 160 CFU/ml in fresh and frozen semen, respectively. Jersey bull showed significantly higher bacterial count (p < 0.05 both in fresh (4.07 × 104 ± 13927 CFU/ ml and frozen (1.92 × 103 ± 178 CFU/ml semen followed by HF cross, Sahiwal, Gir, Red Sindhi and Tharparkar bull. Bulls aged < 4 years and more than 6 years yielded increased bacterial load in their semen. Although a minor variation was reported between species and among age groups, no significant differences were measured. Conclusion: Bacterial load in semen did not differ significantly between species and age groups; however significant variation was reported among different breeds. Bulls of Jersey breed showed significantly higher bacterial load in semen as compared to the crossbred and indigenous bull.

  4. Peritonitis bacteriana espontánea: estudio en pacientes cirróticos descompensados con ascitis Spontaneous bacterial peritonitis in decompensated cirrhotic patients with ascites

    Directory of Open Access Journals (Sweden)

    Fernando Montoya Maya

    1995-02-01

    Full Text Available Se realizó un estudio descriptivo prospectivo entre febrero de 1993 y marzo de 1994. Se estudiaron 25 pacientes cirróticos (3 de ellos en dos ocasiones con ascitis clínica o ecográfica que ingresaron al servicio de Medicina Interna del Hospital Universitario San Vicente de Paúl, Medellín, Colombia, por descompensación de su cuadro clínico. A todos se les practicó paracentesis diagnóstica con estudio de citoquímico, citología y cultivo para bacterias aerobias y anaerobias. La edad promedio de los pacientes fue de 43.9 años (13 a 77 afíos, 13 fueron mujeres (52% y 12 hombres (48%. En 11 episodios (39.3% se encontró ascitis infectada, así: ascitis bacteriana 6 casos (54.6% (monomicrobiana en tres y polimicrobiana en otros tres; peritonitis bacteriana espontánea 3 casos (27.2% (polimicrobiana en dos y monomicrobiana en uno y ascitis neutrofílica dos casos (18.2%. El 72.7% de los casos con ascitis infectada tuvieron fiebre contra un 23.5% de aquéllos sin infección (p < 0.014. Asimismo, el dolor abdominal fue un síntoma bastante frecuente (45.5% de infección. La ascitis infectada se presentó únicamente en individuos categoría C de la clasificación de Child-Pugh, con niveles séricos de albúmina, generalmente menores de 2 g/dl (p < 0.01. Los niveles de albúmina en líquido ascítico menores de 1 g/dl no se asociaron a la infección del mismo. Los gérmenes prevalentes fueron Escherichia coli y estreptococos del grupo viridans, cada uno aislado en 5 ocasiones. Los aislamientos fueron polimicrobianos en 5 de nueve casos (55.5%. La mortalidad por ascitis infectada fue de 27.3% (3/11 casos comparable con la debida a causas diferentes (29.4% en individuos con ascitis sin infección (5/17. Se concluye que la infección del líquido ascítico es una complicación frecuente en los pacientes cirróticos descompensados. La fiebre y el dolor abdominal son los signos clínicos cardinales de la infección. El aislamiento frecuente de E

  5. Bioactivity-guided fractionation identifies amygdalin as a potent neurotrophic agent from herbal medicine semen persicae extract

    OpenAIRE

    Chuanbin Yang; Jia Zhao; Yuanyuan Cheng; Xuechen Li; Jianhui Rong

    2014-01-01

    Herbal medicine Semen Persicae is widely used to treat blood stasis in Chinese medicine and other oriental folk medicines. Although little is known about the effects of Semen Persicae and its active compounds on neuron differentiation, our pilot study showed that Semen Persicae extract promoted neurite outgrowth in rat dopaminergic PC12 cells. In the present study, we developed a bioactivity-guided fractionation procedure for the characterization of the neurotrophic activity of Semen Persicae...

  6. Efecto del metil paratión en la diversidad bacteriana presente en el suelo de una chinampa de Xochimilco /

    OpenAIRE

    Chávez López, Claudia

    2012-01-01

     tesis que para obtener el grado de Doctorado en Ciencias Biológicas, presenta Claudia Chávez López ; asesor Nathalie Cabirol, Silke Cram Heydrich, Francisco José Fernández Perrino89 páginas : ilustracionesDoctorado en Ciencias Biológicas UNAM, Instituto de Ecología, 2012

  7. Effect of sexed-semen use on Holstein conception rate, calf sex, dystocia, and stillbirth in the United States

    Science.gov (United States)

    Most artificial-insemination organizations in the United States now market sex-sorted semen. For 10.8 million US Holstein breedings with conventional semen since January 2006 and 122,705 sexed-semen breedings, data were available from all breedings for conception rate, 12 and 9% of breedings for cal...

  8. 9 CFR 98.38 - Restrictions on the importation of swine semen from the APHIS-defined EU CSF region.

    Science.gov (United States)

    2010-01-01

    ... swine semen from the APHIS-defined EU CSF region. 98.38 Section 98.38 Animals and Animal Products ANIMAL... Semen § 98.38 Restrictions on the importation of swine semen from the APHIS-defined EU CSF region. In...-defined EU CSF region must meet the following conditions, except as noted in paragraph (h) of this...

  9. Does last week's alcohol intake affect semen quality or reproductive hormones?

    DEFF Research Database (Denmark)

    Hansen, Mette Lausten; Thulstrup, A M; Bonde, Jens Peter;

    2012-01-01

    The association between last 5 days of alcohol intake, semen quality and reproductive hormones was estimated in this cross-sectional study among 347 men. Conventional semen characteristics, DNA fragmentation index and reproductive hormones (testosterone, estradiol, sex hormone-binding globulin...

  10. Semen quality in adult male survivors 5 years after the 2008 Wenchuan earthquake.

    Science.gov (United States)

    Chen, X M; Chen, S M; Yue, H X; Lin, L; Wu, Y B; Liu, B; Jiang, M; Ma, Y X

    2016-12-01

    The influence of the Wenchuan earthquake on semen quality of adult male survivors is unclear. We investigated the semen quality included 673 male survivors from the worse-affected counties in the earthquake between Aug 2008 and July 2013. Semen parameters including pH, volume, concentration, motility and morphology were measured according to the World Health Organization (WHO) criteria. Kruskal-Wallis analysis of variance was used to examine the statistical differences between years, and a logistic regression was used to analyse the impacts caused by earthquake on the changes of semen quality. We found the medians (5th and 95th) were 2.5 ml (0.6-5.5) for semen volume, 59.0 × 10(6)  ml(-1) [(13.0-133.0)] × 10(6)  ml(-1) for semen concentration, 46% (13-64%) for sperm progressive motility and 3.0% (0-17.5%) for normal morphology for adult male survivors. Semen concentration, the percentage of sperm progressive motility, total motility and sperm normal morphology were all decreased in the first 3 years, and the differences among years 1, 2 and 3 were significant except the percentage of sperm progressive motility (P earthquake had a negative effect on semen quality. The main findings will provide further diagnosis and therapy basis of male fertility by data, for affected populations in the earthquake.

  11. ANALISIS PENGARUH STRATEGI PEMASARAN DALAM PENINGKATAN PANGSA PASAR PADA PT. SEMEN TONASA

    OpenAIRE

    ARIANSYAH, IRWIN

    2014-01-01

    2014 ABSTRAK ANALISIS PENGARUH STRATEGI PEMASARAN DALAM PENINGKATAN PANGSA PASAR PADA PT. SEMEN TONASA Analysis Affect of Marketing Strategy in Increasing of Market Share on Semen Tonasa Ltd. Irwin Ariansyah H. Cepi Pahlevi Wardhani Hakim Penelitian ini bertujuan untuk mengkaji dan menganalisis pengaruh strategi pemasaran yang terdiri atas segmentasi, target dan posisi pasar terhadap peningkatan pangsa pas...

  12. Semen quality of male partners of infertile couples living with HIV infection

    Directory of Open Access Journals (Sweden)

    Oliver Chukwujekwu Ezechi

    2016-05-01

    Conclusions: Male factor contributes about one third of infertility in this study and severity of HIV diseases impacts on semen quality. Further studies are needed to evaluate if antiretroviral therapy will reverse the effect of HIV infection on semen quality. [Int J Reprod Contracept Obstet Gynecol 2016; 5(5.000: 1423-1427

  13. Application of computer-assisted semen analysis to explain variations in pig fertility.

    NARCIS (Netherlands)

    Broekhuijse, M.L.W.J.; Sostaric, E.; Feitsma, H.; Gadella, B.M.

    2012-01-01

    Sperm quality is often evaluated through computer-assisted semen analysis (CASA) and is an indicator of boar fertility. The aim of this research was to study the relationship between CASA motility parameters and fertility results in pigs. Insemination records and semen parameters from a total of 45,

  14. Phthalate Exposure and Human Semen Quality in Shanghai A Cross-sectional Study

    Institute of Scientific and Technical Information of China (English)

    YUN-HUI ZHANG; LI-XING ZHENG; BING-HENG CHEN

    2006-01-01

    Objective To monitor the level of phthalates in human semen samples and to analyze the relationship between phthalate levels and semen parameters. Methods Concentrations of three kinds of commonly used phthalates (di-ethyl phthalate, DEP; di-n-butyl phthalate, DBP; di-2-ethylhexyl phthalate, DEHP) were measured using reversed-phase HPLC. Semen parameters were measured by computer aided sperm analysis (CASA). Results The three phthalates were detected in most of the biological samples, With median levels of 0.30 mg/L (0.08-1.32 mg/L) in semen specimens. There was a significant positive association between liquefied time of semen and phthalate concentrations of semen. The correlation coefficient was 0.456 for DEP, 0.475 for DBP, and 0.457 for DEHP, respectively. There was no significant difference between phthalate concentrations of semen and sperm density or livability, though the correlation coefficients were negative. Conclusion These results suggest that people who reside in Shanghai are exposed to phthalates, especially to DBP and DEHP. Although the level of phthalates is relatively mild, an association of phthalate levels and reduced quality of human semen has been shown in the present study.

  15. Schmallenberg virus detection in bovine semen after experimental infection of bulls.

    NARCIS (Netherlands)

    Poel, van der W.H.M.; Parlevliet, J.M.; Verstraten, E.R.A.M.; Kooi, E.A.; Hakze-van der Honing, van der R.W.; Stockhofe-Zurwieden, N.

    2014-01-01

    To study Schmallenberg virus (SBV) excretion in bovine semen after experimental infection, two bulls were inoculated subcutaneously with a SBV isolate (1 ml Vero cell culture 106 TCID50). After inoculation (at day 0), semen was collected daily from both animals for 21 days and samples were tested fo

  16. [Infrared spectrum analysis of admixture decoction of herba ephedrae with semen armeniacae amarum].

    Science.gov (United States)

    Lin, Wen-Shuo; Chen, Rong; Guo, Shao-zhong; Lin, Ju-qiang; Feng, Shang-yuan; Li, Yong-zeng; Huang, Zu-fang; Cai, Yu-hui

    2008-12-01

    The infrared spectra of decoction of herba ephedra and semen armeniacae amarum and the mixed decoction of herba ephedra + semen armeniacae amarum were tested. The change in the the mixed decoction was discussed to study the relationship between herba ephedra and semen armeniacae amarum after decoction. The results showed that some absorption peaks of herba ephedra and semen armeniacae amarum were retained in the mixed decoction of herba ephedra + semen armeniacae amarum, such as 1402 and 1076 cm(-1), but some absorption peaks that never appear in the two ingredient spectra increased such as 1394 and 682 cm(-1). New absorption peaks were generated in the mixed decoction of herba ephedra + semen armeniacae amarum, such as 688 and 1187 cm(-1). It can be showed that there were differences in the chemistry environment of the various chemical groups in the three decoctions introduced above, with the variation in absorption peak position, and the biochemical structure of the material changed, possibly with some new chemical compositions created. Medical ingredients in the mixed decoction of herba ephedra + semen armeniacae amarum were not simply the addition of herba ephedra and semen armeniacae amarum based on studies of infrared spectrum of decoction, and the new notion of prescription spectroscopy was proposed.

  17. Resazurin reduction and other tests of semen quality and fertility of bulls

    Institute of Scientific and Technical Information of China (English)

    RobertH.Foote

    1999-01-01

    Aim: This study was undertaken to compare the reduction in color of two dyes methylene blue (MBRT) anddye (RRT) with other tests of bull semen quality and to examine their relationship to fertility. Methods: One hundredsixty-four ejaculates from 59 bulls were examined, processed, fertility, and semen from these bulls averaged

  18. [Evaluation of the quality of the human spermatozoon: comparison between spermatic DNA integrity and semen variables].

    Science.gov (United States)

    Cruz, Ibis; Colmenares, Melisa; Berrueta-Carrillo, Leidith; Gomez-Perez, Roald; Montes, Henry; Berrueta, Lisbeth; Salmen, Siham; Osuna, Jesús Alfonso

    2010-03-01

    Semen analysis does not have an absolute predictive value on fertility, however it is a reflection of male fertility potential, which is related to its spermatozoa quality and other semen variables. Great variability in human semen parameters has been demonstrated within a single individual, an observation that could explain why a male with low semen quality can successfully fertilize an egg. Although conventional semen analysis, such as sperm concentration, motility and morphology, provide important information about the clinical status of male fertility, new procedures to predict the sperm functional capability have been developed in the last decade, such as analysis of nuclear DNA integrity, which have improved considerably the clinical diagnosis of male infertility, and increased the knowledge about spermatozoa function. DNA fragmentation consist in interruptions, both in single and double DNA strains, that frequently occur in sperm samples from infertile patients. We have conducted a clinical study in semen samples from patients who have attended the Andrology laboratory of the University of Los Andes, between March 2007 and March 2009. The aim of this study was to compare sperm DNA integrity, analyzed by flow cytometry, with traditional semen parameters. Our results show remarkable correlations between conventional human semen variables and sperm chromatin integrity, contributing to asses an integral evaluation of sperm quality allowing the analysis of its fertilizing potential in clinical studies.

  19. Ebola Virus RNA in Semen from an HIV-Positive Survivor of Ebola

    Science.gov (United States)

    Rogers, Emerson; Baller, April; White, Stephen; Soka, Moses; Choi, Mary J.; Mahmoud, Nuha; Wasunna, Christine; Massaquoi, Moses; Kollie, Jomah; Dweh, Straker; Bemah, Philip; Ladele, Victor; Kpaka, Jonathan; Jawara, Mary; Mugisha, Margaret; Subah, Onyekachi; Faikai, Mylene; Bailey, Jeff A.; Rollin, Pierre; Marston, Barbara; Nyenswah, Tolbert; Gasasira, Alex; Knust, Barbara; Nichol, Stuart; Williams, Desmond

    2017-01-01

    Ebola virus is known to persist in semen of male survivors of Ebola virus disease (EVD). However, maximum duration of, or risk factors for, virus persistence are unknown. We report an EVD survivor with preexisting HIV infection, whose semen was positive for Ebola virus RNA 565 days after recovery from EVD. PMID:28287374

  20. Computer-assisted semen analysis parameters as predictors for fertility of men from the general population

    DEFF Research Database (Denmark)

    Larsen, L; Scheike, Thomas Harder; Jensen, Tina Kold;

    2000-01-01

    The predictive value of sperm motility parameters obtained by computer-assisted semen analysis (CASA) was evaluated for the fertility of men from general population. In a prospective study with couples stopping use of contraception in order to try to conceive, CASA was performed on semen samples...

  1. Efecto bactericida del láser Nd: YAP. Estudio in vitro Bactericidal effects of Nd: YAP. An in vitro study

    OpenAIRE

    G Caccianiga; Urso, E.; R Monguzzi; Gallo, K.; G. Rey

    2007-01-01

    La utilización del láser en el ambiente odontológico está teniendo cada vez más difusión gracias al hecho que éste puede conciliar un elevado standard de confort para el paciente con la eficacia terapéutica. El presente estudio ha evaluado la eficacia bactericida de la radiación láser asociada al empleo del agua oxigenada respecto a cinco cepas bacterianas comúnmente presentes en las bolsas periodontales activas y resistentes al empleo separado del láser y del agua oxigenada. Las cinco bacter...

  2. Application of a quantitative 1H-NMR method for the determination of amygdalin in Persicae semen, Armeniacae semen, and Mume fructus.

    Science.gov (United States)

    Tanaka, Rie; Nitta, Akane; Nagatsu, Akito

    2014-01-01

    A quantitative (1)H-NMR method (qHNMR) was used to measure the amygdalin content of Persicae semen, Armeniacae semen, and Mume fructus, in each of which amygdalin constitutes a major component. The purity of amygdalin was calculated from the ratio of the intensity of the amygdalin H-2 signal at δ 6.50 ppm in pyridine-d 5 to that of the hexamethyldisilane (HMD) signal at 0 ppm. The HMD concentration was corrected by the International System of Units (SI) traceability with certified reference material (CRM)-grade bisphenol A. qHNMR revealed the amygdalin contents to be 2.72 and 3.13% in 2 lots of Persicae semen, 3.62 and 5.19% in 2 lots of Armeniacae semen, and 0.23% in Mume fructus. Thus, we demonstrated the utility of this method for the quantitative analysis of crude drugs.

  3. Presence and Persistence of Zika Virus RNA in Semen, United Kingdom, 2016

    Science.gov (United States)

    Atkinson, Barry; Thorburn, Fiona; Petridou, Christina; Bailey, Daniel; Hewson, Roger; Simpson, Andrew J.H.; Brooks, Timothy J.G.

    2017-01-01

    Zika virus RNA has been detected in semen collected several months after onset of symptoms of infection. Given the potential for sexual transmission of Zika virus and for serious fetal abnormalities resulting from infection during pregnancy, information regarding the persistence of Zika virus in semen is critical for advancing our understanding of potential risks. We tested serial semen samples from symptomatic male patients in the United Kingdom who had a diagnosis of imported Zika virus infection. Among the initial semen samples from 23 patients, Zika virus RNA was detected at high levels in 13 (56.5%) and was not detected in 9 (39.1%); detection was indeterminate in 1 sample (4.4%). After symptomatic infection, a substantial proportion of men have detectable Zika virus RNA at high copy numbers in semen during early convalescence, suggesting high risk for sexual transmission. Viral RNA clearance times are not consistent and can be prolonged. PMID:27997333

  4. Air pollution and decreased semen quality: a comparative study of Chongqing urban and rural areas.

    Science.gov (United States)

    Zhou, Niya; Cui, Zhihong; Yang, Sanming; Han, Xue; Chen, Gangcai; Zhou, Ziyuan; Zhai, Chongzhi; Ma, Mingfu; Li, Lianbing; Cai, Min; Li, Yafei; Ao, Lin; Shu, Weiqun; Liu, Jinyi; Cao, Jia

    2014-04-01

    To investigate the association and effects of air pollution level on male semen quality in urban and rural areas, this study examines the outdoor concentrations of particulate matter (PM10), sulfur dioxide (SO2), nitrous dioxide (NO2) and semen quality outcomes for 1346 volunteers in both urban and rural areas in Chongqing, China. We found the urban area has a higher pollution level than the rural area, contrasted with better semen quality in the rural residents, especially for sperm morphology and computer assistant semen analysis (CASA) motility parameters. A multivariate linear regression analysis demonstrates that concentrations of PM10, SO2, and NO2 significantly and negatively are associated with normal sperm morphology percentage (P urban ambient air may account for worse semen quality in urban males.

  5. Successful ram semen cryopreservation with lyophilized egg yolk-based extender.

    Science.gov (United States)

    Alcay, Selim; Berk Toker, M; Gokce, Elif; Ustuner, Burcu; Tekin Onder, N; Sagirkaya, Hakan; Nur, Zekariya; Kemal Soylu, M

    2015-10-01

    The aim of this study was to evaluate the effects of lyophilized egg yolk extender on ram semen cryopreservation. Ejaculates with a thick consistency, rapid wave motion (3-5 on a 0-5 scale) and >75% initial motility were pooled. Sperm were diluted to final concentration of 1/5 (semen/extender) in lyophilized egg yolk or fresh egg yolk extenders using two-step dilution method. The equilibrated semen was frozen in 0.25 mL straws. Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) at three time points: after dilution with extender A, equilibration and post-thaw. The results showed that freezing and thawing procedures (dilution, equilibration and thawing) had negative effects on motility (Pram semen.

  6. Assessment of semen quality in pure and crossbred Jersey bulls

    Directory of Open Access Journals (Sweden)

    Umesh Kumar

    2015-10-01

    Full Text Available Aim: To compare the seminal attributes of neat, pre-freeze (at equilibration, and post-freeze (24 h after freezing semen in pure and crossbred Jersey bulls. Materials and Methods: Total 36 ejaculates (3 ejaculates from each bull were collected from 6 pure Jersey and 6 crossbred Jersey bulls and evaluated for various seminal attributes during neat, pre-freeze, and post-freeze semen. Results: The mean (±standard error [SE] values of neat semen characteristics in pure and crossbred Jersey bulls were recorded such as volume (ml, color, consistency, mass activity (scale: 0-5, and sperm concentration (millions/ml. The extended semen was further investigated at pre-freeze and post-freeze stages and the mean (±SE values recorded at neat, pre-freeze, and post-freeze semen were compared between pure and crossbred Jersey bulls; sperm motility (80.55±1.70%, 62.77±1.35%, 46.11±1.43% vs. 80.00±1.80%, 65.00±1.66%, 47.22±1.08%, live sperm count (83.63±1.08%, 71.72±1.09%, 58.67±1.02% vs. 80.00±1.08%, 67.91±1.20%, 51.63±0.97%, total abnormal sperm count (8.38±0.32%, 12.30±0.39%, 16.75±0.42% vs. 9.00±0.45%, 12.19±0.48%, 18.11±0.64%, hypo-osmotic swelling (HOS reacted spermatozoa (71.88±0.77%, 62.05±0.80%, 47.27±1.05% vs. 72.77±1.02%, 62.11±0.89%, 45.94±1.33%, acrosome integrity (89.05±0.83%, 81.33±0.71%, 71.94±0.86% vs. 86.55±0.57%, 78.66±0.42%, 69.38±0.53%, and DNA integrity (99.88±0.07%, 100, 99.66±0.11% vs. 99.94±0.05%, 100, 99.44±0.18%,. The volume, color, consistency, sperm concentration, and initial motility in pure and crossbred Jersey bulls did not differ significantly (p>0.05. The mass activity was significantly (p0.05 was observed in abnormal sperm; HOS reacted spermatozoa and DNA integrity percentage among breeds. Conclusion: It may be concluded that the quality of pure Jersey bull semen was comparatively better than the crossbred Jersey bulls.

  7. Expresiones del CD44 en tejidos periapicales inflamados.

    OpenAIRE

    Siragusa, Martha; Dietrich, Gustavo; Pisterna, Gabriela; D'Arrigo, Mabel

    2012-01-01

    Recibido: Noviembre 2011 - Aceptado: Marzo 2012 El granuloma periapical es una lesión inflamatoria crónica causada por una infección poli bacteriana. Las moléculas de adhesión del CD44 están fuertemente expresadas en células variadas tales como los leucocitos, células parenquimatosas e incluyendo células endoteliales, en células epiteliales y en células musculares. Tiene interacción con el Acido Hialurónico, con el colágeno, lamininas y fibronectina y juega un rol importante en la migraci...

  8. Principal Component Analysis on Semen Quality among Chinese Young Men

    Institute of Scientific and Technical Information of China (English)

    Jun-qing WU; Er-sheng GAO; Jian-guo TAO; Cui-ling LIANG; Wenying LI; Qiu-ying YANG; Kang-shou YAO; Wei-qun LU; Lu CHEN

    2003-01-01

    Objective To understand the current semen quality status among Chinese young men and influential factors in China and to explore its evaluation index. Methods A total of 562 healthy male volunteers were recruited during their premarital examinations in seven provincials and municipal regions' MCH centers; descriptive and principal component analyses were used to analyze data.Results The findings show that semen volume (2.61±1.10 mL), sperm density (64.47±34.59×106/mL), percentage of sperm forward progression (59.89%±17.11%), percentage of sperm viability (77.19%±11.87%), and percentage of normal sperm morphology (78.23%±9.15%). The first principal component function is Z1=-8.512 54 + 0.001 36X1' +0.031 92X2'+0.043 52X3'+ 0.039 84X4', which is closely related to percentage of sperm viability (X3), percentage of sperm forward progression (X2), and percentage of normal sperm morphology (X4);The second principal component function is: Z2=0.491 92+ 0.080 80X1- 0.000 58X2 - 0.005 10X3 - 0.018 07X4, which depends on the total sperm count (X1). Conclusion Only 42.3% subjects meet all the common WHO standard of semen quality. The multiple analysis of Z1 showed that the highest Z1 are among subjects from Guizhou, workers, or town residents. Multiple analysis of Z2 showed that the older age when the subjects had the first sexual impulse, the longer period of sexual abstinence and more quantity of sperm they had; the more sexual activity subjects had, the less amount of sperm they had.

  9. Freezing African elephant semen as a new population management tool.

    Directory of Open Access Journals (Sweden)

    Robert Hermes

    Full Text Available BACKGROUND: The captive elephant population is not self-sustaining and with a limited number of breeding bulls, its genetic diversity is in decline. One way to overcome this is to import young and healthy animals from the wild. We introduce here a more sustainable alternative method - importation of semen from wild bulls without removing them from their natural habitat. Due to the logistics involved, the only practical option would be to transport cryopreserved sperm. Despite some early reports on African elephant semen cryopreservation, the utility of this new population management tool has not been evaluated. METHODOLOGY/PRINCIPAL FINDINGS: Semen was collected by electroejaculation from 14 wild African savanna elephant (Loxodonta africana bulls and cryopreserved using the directional freezing technique. Sperm treatments evaluated included the need for centrifugation, the use of hen or quail yolk, the concentration of glycerol (3%, 5% or 7% in the extender, and maintenance of motility over time after thawing. Our results suggest that dilution in an extender containing hen yolk and 7% glycerol after centrifugation best preserved post-thaw sperm motility when compared to all other treatments (P≤0.012 for all. Using this approach we were able to achieve after thawing (mean ± SD 54.6±3.9% motility, 85.3±2.4% acrosome integrity, and 86.8±4.6% normal morphology with no decrease in motility over 1 h incubation at 37°C. Sperm cryopreserved during this study has already lead to a pregnancy of a captive female elephant following artificial insemination. CONCLUSIONS/SIGNIFICANCE: With working techniques for artificial insemination and sperm cryopreservation of both African and Asian elephants in hand, population managers can now enrich captive or isolated wild elephant populations without removing valuable individuals from their natural habitat.

  10. EFFECT OF TWO DIFFERENT ANTIBIOTIC COMBINATIONS ON FERTILITY OF FROZEN BUFFALO AND SAHIW AL BULL SEMEN

    Directory of Open Access Journals (Sweden)

    S.M.H. Andrabi, N. Ahmad, A. Abbas and M. Anzar

    2001-09-01

    Full Text Available This study was carried out to identify the suitable antibiotic combinations in semen extender for improvement in fertility of frozen semen of buffalo and cow (Sahiwal bulls to obtain better pregnancy rate through artificial insemination (AI. For this study eight first ejaculates, four each from a buffalo and a cow (Sahiwal bull were used. The ejaculates were split-sampled and diluted with Tris-citric acid extender (at 37°C; 50x 106 spermatozoa/mI, containing either SP (streptomycin 1000 ~g/ml and penicillin 1000 IU/ml or GTLS (gentamycin 500 µg/ml, Tylosin 100 µg/ml and linco-spectin 300/600 µg/ml. There was no difference in post-thaw motility for these samples. Fertility test based on 75-days first service pregnancy rate was determined under field conditions. A total of 400 inseminations were recorded, 200 for each buffalo and cow (Sahiwal with J 00 of each antibiotic combination, respectively. Fertility rates for SP-based frozen semen of buffalo bull were 41.66% and were 55.2% for GTLS-containing frozen semen, respectively. The results for GTLS were higher (P<0.0001 than SP. Similarly, fertility rates were higher (P<0.0001 for GTLS-based frozen semen of Sahiwal bull (78.78% than SP-containing frozen semen (69.6% of the same specie. Fertility rates also differed due to species of donor bulls. They were better (P<0.0001 for the frozen Sahiwal bull semen than that of the buffalo bull in both SP and GTLS- based frozen semen samples, respectively. In conclusion. seminal quality measured by field fertility trial indicated GTLS combination of antibiotics added to the semen extender was better for improvement in the fertility of frozen buffalo and Sahiwal bull semen, by yielding better pregnancy rates through AI.

  11. Semen Bacterial Concentrations and HIV-1 RNA Shedding Among HIV-1–Seropositive Kenyan Men

    Science.gov (United States)

    Srinivasan, Sujatha; Huang, Dandi; Ko, Daisy L.; Sanders, Eduard J.; Peshu, Norbert M.; Krieger, John N.; Muller, Charles H.; Coombs, Robert W.; Fredricks, David N.; Graham, Susan M.

    2017-01-01

    Introduction: HIV-1 is transmitted through semen from men to their sexual partners. Genital infections can increase HIV-1 RNA shedding in semen, but shedding also occurs in the absence of typical pathogens. We hypothesized that higher bacterial concentrations in semen would be associated with higher HIV-1 RNA levels. Methods: We analyzed semen samples from 42 HIV-1–seropositive Kenyan men using quantitative polymerase chain reaction (PCR) to assess bacterial concentrations and real-time PCR to measure HIV-1 RNA levels. Generalized estimation equations were used to evaluate associations between these 2 measures. Broad-range 16S rRNA gene PCR with pyrosequencing was performed on a subset of 13 samples to assess bacterial community composition. Results: Bacteria were detected in 96.6% of 88 samples by quantitative PCR. Semen bacterial concentration and HIV-1 RNA levels were correlated 0.30 (P = 0.01). The association between bacterial concentration and HIV-1 RNA detection was not significant after adjustment for antiretroviral therapy (ART) (adjusted odds ratio: 1.27, 95% CI: 0.84 to 1.91). Factors associated with semen bacterial concentration included insertive anal sex (adjusted beta 0.92, 95% CI: 0.12 to 1.73) and ART use (adjusted beta: −0.77, 95% CI: −1.50 to 0.04). Among 13 samples with pyrosequencing data, Corynebacterium spp., Staphylococcus spp., and Streptococcus spp. were most frequently detected. Conclusion: Most of these HIV-1–infected men had bacteria in their semen. ART use was associated with undetectable semen HIV-1 RNA and lower semen bacterial concentrations, whereas insertive anal sex was associated with higher bacterial concentrations. Additional studies evaluating the relationship between semen bacteria, inflammation, mucosal immunity, and HIV-1 shedding are needed to understand implications for HIV-1 transmission. PMID:27861240

  12. Successful artificial insemination in the Asian elephant (Elephas maximus using chilled and frozen-thawed semen

    Directory of Open Access Journals (Sweden)

    Wongkalasin Warut

    2009-07-01

    Full Text Available Abstract Background Artificial insemination (AI using frozen-thawed semen is well established and routinely used for breeding in various mammalian species. However, there is no report of the birth of elephant calves following AI with frozen-thawed semen. The objective of the present study was to investigate the fertilizing ability of chilled and frozen-thawed semen in the Asian elephant following artificial insemination (AI. Methods Semen samples were collected by from 8 bulls (age range, 12-to 42-years by manual stimulation. Semen with high quality were either cooled to 4°C or frozen in liquid nitrogen (-196°C before being used for AI. Blood samples collected from ten elephant females (age range, 12-to 52-years were assessed for estrus cycle and elephants with normal cycling were used for AI. Artificial insemination series were conducted during 2003 to 2008; 55 and 2 AI trials were conducted using frozen-thawed and chilled semen, respectively. Pregnancy was detected using transrectal ultrasonography and serum progestagen measurement. Results One female (Khod inseminated with chilled semen became pregnant and gave birth in 2007. The gestation length was 663 days and the sex of the elephant calf was male. One female (Sao inseminated with frozen-thawed semen showed signs of pregnancy by increasing progestagen levels and a fetus was observed for 5 months by transrectal ultrasonography. Conclusion This is the first report showing pregnancy following AI with frozen-thawed semen in the Asian elephant. Successful AI in the Asian elephant using either chilled or frozen-thawed semen is a stepping stone towards applying this technology for genetic improvement of the elephant population.

  13. Twin pregnancy possibly associated with high semen quality

    DEFF Research Database (Denmark)

    Asklund, Camilla; Jensen, Tina Kold; Jørgensen, Niels

    2007-01-01

    a questionnaire. RESULTS: After adjustment, fathers of DZ and MZ twins had 3.6 (95% CI 1.7; 5.4) and 4.6 (95% CI 2.0; 7.2) percentage points higher percentage of sperm cells with normal morphologic features and percentages of motile sperm cells were 11.5 (95% CI 7.2; 15.9) and 12.5 (95% CI 6.3; 18.6) percentage...... points higher than the reference group (P sperm concentration than the reference group. CONCLUSIONS: Fathers of DZ twins had a better semen quality than the reference group, which supports...

  14. Analisa Pengelolaan Modal Kerja pada Perusahaan Industri Semen

    Directory of Open Access Journals (Sweden)

    Engelwati Gani

    2011-04-01

    Full Text Available The purpose of this research is to identify financial ratios, to analyse the relation among the financial ratios, and to compare financial ratios of PT Indocement Tunggal Prakarsa, Tbk., PT Semen Gresik (Persero, Tbk., dan PT Holcim Indonesia, Tbk. The research method is qualitative, descriptive research, with the research time dimension using time series. The research depth is using the three industrial cement companies that listed in Indonesia Stock Exchange (BEJ. The gathering data method is by indirect contact like archive data.

  15. Analisa Pengelolaan Modal Kerja pada Perusahaan Industri Semen

    Directory of Open Access Journals (Sweden)

    Engelwati Gani

    2011-05-01

    Full Text Available The purpose of this research is to identify financial ratios, to analyse the relation among the financial ratios, and to compare financial ratios of PT Indocement Tunggal Prakarsa, Tbk., PT Semen Gresik (Persero, Tbk., dan PT Holcim Indonesia, Tbk. The research method is qualitative, descriptive research, with the research time dimention using time series. The research depth is using the three industrial cement companies that listed in Indonesia Stock Exchange (BEJ. The gathering data method is by indirect contact like archive data. 

  16. LEONARDO DA VINCI AND THE ORIGIN OF SEMEN.

    Science.gov (United States)

    Noble, Denis; DiFrancesco, Dario; Zancani, Diego

    2014-12-20

    It is well known that Leonardo da Vinci made several drawings of the human male anatomy. The early drawings (before 1500) were incorrect in identifying the origin of semen, where he followed accepted teaching of his time. It is widely thought that he did not correct this mistake, a view that is reflected in several biographies. In fact, he made a later drawing (after 1500) in which the description of the anatomy is remarkably accurate and must have been based on careful dissection. In addition to highlighting this fact, acknowledged previously in only one other source, this article reviews the background to Leonardo's knowledge of the relevant anatomy.

  17. CORRELATION BETWEEN HYPO-OSMOTIC SWELLING TEST AND VARIOUS CONVENTIONAL SEMEN EVALUATION PARAMETERS IN FRESH NILI-RAVI BUFFALO AND SAHIWAL COW BULL SEMEN

    Directory of Open Access Journals (Sweden)

    L. A. LODHI, M. ZUBAIR, Z. I. QURESHI, I. AHMAD AND H. JAMIL

    2008-12-01

    Full Text Available The present study was conducted to determine the correlation of hypo-osmotic swelling test with conventional semen evaluation parameters of fresh semen collected from two Nili-Ravi buffalo and two Sahiwal cow bulls. A total of 10 pooled samples (each comprising two consecutive ejaculates from each bull were collected. Each semen sample was divided into two parts. One part was used for the evaluation of semen by conventional method, while the other part was subjected to hypo-osmotic swelling test by using 150 mOsm/L sodium citrate fructose solution. The mean sperm positive to HOS test was 85.25% both in Nili-Ravi buffalo and Sahiwal cow bull semen. Statistical analysis of the data revealed a significant (P<0.05 positive correlation between progressive motility, morphologically normal spermatozoa, sperm viability and percentage of HOS test positive spermatozoa for both species. It was inferred that HOS test could be a valuable method for routine evaluation of semen for artificial insemination.

  18. Adaptation of ubiquitin-PNA based sperm quality assay for semen evaluation by a conventional flow cytometer and a dedicated platform for flow cytometric semen analysis.

    Science.gov (United States)

    Odhiambo, J F; Sutovsky, M; DeJarnette, J M; Marshall, C; Sutovsky, P

    2011-10-01

    The purpose of semen quality evaluation is to predict the fertility potential of the sample in an objective, rapid and inexpensive manner. However, utilization of sperm quality biomarkers such as ubiquitin and lectin Arachis hypogaea agglutinin (PNA) for flow cytometric semen evaluation might eliminate the need for visual assessment by microscopy. Herein, we demonstrate a robust ubiquitin and PNA-based semen evaluation conducted on a simple, easy to operate, dedicated sperm flow cytometer, EasyCyte Plus (IMV Technologies, L'Aigle, France). Semen samples were collected periodically from two dairy bulls, which were subjected to temporary scrotal insults to induce variable semen quality. Samples were labeled with fluorescently-conjugated anti-ubiquitin antibodies (bind exclusively to the surface of defective sperm) and lectin PNA (binds to acrosomal surface in prematurely capacitated and acrosome-damaged sperm). Fluorescent properties of the samples were measured with a conventional flow cytometer (Becton Dickinson FACScan; Becton Dickinson Corp., Franklin Lakes, NJ, USA) and by the EasyCyte (IMV Technologies) instrument. Data from the two flow cytometers were positively correlated for the percentage of PNA-positive sperm with a damaged acrosome (r = 0.47; P flow cytometric semen evaluation.

  19. Effect of different antioxidant additives in semen diluent on cryopreservability (−196°C) of buffalo semen

    Science.gov (United States)

    Patel, Hardik A.; Siddiquee, G. M.; Chaudhari, Dinesh V.; Suthar, Vishal S.

    2016-01-01

    Aim: The aim of this study was to evaluate the effect of different antioxidant additives in standard tris-fructose-egg yolk-glycerol (TFYG) extender on the cryopreservability of buffalo semen. Materials and Methods: Semen collection using artificial vagina, twice weekly for 5 weeks from three pedigreed health breeding bulls of Mehsani breed, aged between 6 and 8 years. Immediately after initial evaluation all 30 qualifying ejaculates (10/bull) were split into three aliquots and diluted at 34°C keeping the concentration of 100 million spermatozoa/ml with standard TFYG extender as control and TFYG having two antioxidant additives - Cysteine HCl at 1 mg/ml and ascorbic acid at 0.2 mg/ml to study their comparative performance. Semen filled in French Mini straws using IS-4 system and gradually cooled to 4°C and equilibrated for 4 h in cold handing cabinet. After completion of equilibration, straws were cryopreserved in LN2 by Programmable Bio-freezer. Semen was examined at post-dilution, post-equilibration, and post-thaw stages for sperm quality parameters, and at each stage plasma was separated for enzymatic analysis of aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and alkaline phosphatase (AKP). Results: The mean percentage of sperms in TFYG, TFYG + cysteine HCl and TFYG + ascorbic acid diluents at post-thaw stage in terms of progressive motility (52.83±0.52, 57.83±0.52, 57.83±0.52), livability (78.70±0.21, 82.33±0.23, 81.73±0.22), and abnormality (5.43±0.21, 5.03±0.17, 5.23±0.18) varied significantly (p<0.05) between control TFYG and TFYG having antioxidant additives. The mean U/L activities of AST (78.70±0.47, 72.80±0.48, 73.30±0.54), LDH (172.70±0.41, 155.78±0.42, 156.33±0.41), and AKP (103.61±0.34, 90.20±0.34, 91.03±0.34) in semen diluted with TFYG, TFYG + cysteine HCl and TFYG + ascorbic acid diluents at post-thaw stage, respectively, which showed significantly (p<0.05) higher leakage of enzymes in control TFYG than TFYG

  20. Infecção bacteriana em pacientes portadores de Leishmaniase visceral Bacterial infection in patients with visceral leishmaniasis

    Directory of Open Access Journals (Sweden)

    Jaqueline Guerreiro

    1985-12-01

    Full Text Available Uma análise retrospectiva de 63 casos de Leishmaniose visceral (L.V. revelou a presença, em 33 deles, de infecção bacteriana associada. Infecções do trato respiratório foram observadas em 13 (39,3% pacientes, comprometimento de pele em 4 (12%, do trato urinário em 4 (12%, do ouvido em 3 (9%, e de orofaringe em 2 (6%. Sete (21% pacientes apresentaram infecção concomitante em múltiplos sítios. Documentação bacteriológica através de isolamneto do agente etiológico foi obtida em 10, não havendo predominância estatisticamente significante de bactérias Gram positivas ou negativas. Houve 9 casos de óbito nestes 63 pacientes, sendo que em 8 deles a infecção bacteriana fazia parte do quadro clínico final. A análise das taxas de globulinas séricas revelou que infecção esteve presente de modo significativo (p 0.05 com relação ao número de neutrófilos entre os grupos com e sem infecção bacteriana. Concluiu-se, portanto, que infecção bacteriana é um achado freqüente em pacientes com L.V. e se constitui num sinal de mau prognóstico da doença.In an analysis of 63 hospitalized cases with visceral leishmaniasis, the clinical or post-mortem diagnosis of bacterial infection was performed in 33; 13 (39.3% patients had respiratory infection, 4 (12.1% had skin infection, 4 had urinary tract infection, 3 (9.0% showed ear infection and 2 (6.6% had infection of the oral cavity. It is worth mentioning that in 7 (21% cases there was infection in multiple sites. Gram positive and/or Gram negative organisms were isolated from 10 patients. In only two (autopsied cases, infection with less common organisms was recorded, one with disseminated candidiasis and another with disseminated tuberculosis. Death occurred in 9 of the 63 cases, and in 8 of these, concomitant bacterial infection of importance was documented. Patients who had serum globulins lower than 4 g% had significantly more infection (p less than 0.05 than patients with

  1. Controlo da placa bacteriana em reabilitações implanto-suportadas: revisão da literatura

    OpenAIRE

    Marmelo, Catarina Filipa Dias

    2016-01-01

    Trabalho final do 5º ano com vista à atribuição do grau de mestre no âmbito do ciclo de estudos de Mestrado Integrado em Medicina Dentária apresentado à Faculdade de Medicina da Universidade de Coimbra. Introdução: A reabilitação oral com implantes tem tido uma divulgação exponencial graças aos seus resultados, estéticos e funcionais, altamente satisfatórios. A falta de motivação para a higiene oral e de destreza manual por parte do doente levam à acumulação de placa bacteriana, p...

  2. Cuerpos de inclusión, células bacterianas y composiciones que los contienen y sus usos

    OpenAIRE

    Veciana Miró, Jaume; Ratera Bastardas, Inmaculada; Díez Gil, César; Villaverde Corrales, Antonio Pedro; Vázquez Gómez, Esther; García Fruitós, Elena

    2008-01-01

    [ES] La presente invención se refiere a un cuerpo de inclusión aislado que comprende un polipéptido caracterizado porque el cuerpo de inclusión está en forma particulada. La presente invención también se refiere a una célula bacteriana que comprende dicho cuerpo de inclusión. La presente invención se refiere además a una composición que comprende dicho cuerpo de inclusión y una célula eucariota. La presente invención se refiere también a una composición que comprende dicho cuerpo de ...

  3. Sobre la resistencia bacteriana hacia antibióticos de acción bactericida y bacteriostática

    Directory of Open Access Journals (Sweden)

    Jhoana P. Romero L.

    2014-01-01

    Full Text Available En este artículo se formula un modelo matemático simple que descri- be la interacción entre bacterias sensibles y resistentes a múltiples antibióticos de acción bactericida y bacteriostática de forma simultánea, en el supuesto de que la adquisición de resistencia bacteriana se da a través de mutaciones espontáneas y adquiridas por la exposición a diferentes antibióticos. El análisis cualitativo revela la existencia de un equilibrio libre de bacterias, un equili- brio solo con bacterias resistentes y un equilibrio endémico donde coexisten ambas poblaciones de bacterias.

  4. Effect of magnetized extender on sperm membrane integrity and development of oocytes in vitro fertilized with liquid storage boar semen.

    Science.gov (United States)

    Lee, Sang-Hee; Park, Choon-Keun

    2015-03-01

    The objective of this study was to evaluate the effect of a magnetized extender on sperm membrane damage and development of oocytes in vitro fertilized with liquid storage boar semen. Before semen dilution, extender was flowed through a neodymium magnet (0, 2000, 4000 and 6000G) for 5min and collected semen was preserved for 168h at 18°C. In results, plasma membrane integrity with live sperm was significantly higher in semen treated with extenders magnetized at 4000G than sperm treated with extenders magnetized at 0G during semen preservation for 120-168h (psperm was significantly lower in semen treated with extenders magnetized at 2000G than other groups during semen preservation for 168h. The ability of semen to achieve successful in vitro fertilization was also not significantly different among the groups during preservation. However, when the semen was preserved for 168h, the blastocyst formation rates were significantly higher at 6000G compared to 0 and 2000G (psperm membrane from damage, and improve the ability of rates of in vitro blastocyst development and magnetized semen diluter is beneficial for long liquid preservation of boar semen.

  5. Role of semen in HIV-1 transmission: inhibitor or facilitator?

    Science.gov (United States)

    Doncel, Gustavo F; Joseph, Theresa; Thurman, Andrea R

    2011-03-01

    Sexual transmission of human immunodeficiency virus type 1 (HIV-1) accounts for 60-90% of new infections, especially in developing countries. During male-to-female transmission, the virus is typically deposited in the vagina as cell-free and cell-associated virions carried by semen. But semen is more than just a carrier for HIV-1. Evidence from in vitro and in vivo studies supports both inhibitory and enhancing effects. Intrinsic antiviral activity mediated by cationic antimicrobial peptides, cytotoxicity, and blockage of HIV-dendritic cell interactions are seminal plasma properties that inhibit HIV-1 infection. On the contrary, neutralization of vaginal acidic pH, enhanced virus-target cell attachment by seminal amyloid fibrils, opsonization by complement fragments, and electrostatic interactions are factors that facilitate HIV-1 infection. The end result, i.e., inhibition or enhancement of HIV mucosal infection, in vivo, likely depends on the summation of all these biological effects. More research is needed, especially in animal models, to dissect the role of these factors and establish their relevance in HIV-1 transmission.

  6. APLIKASI PEMETAAN ALIRAN NILAI DI INDUSTRI KEMASAN SEMEN

    Directory of Open Access Journals (Sweden)

    Iwan Vanany

    2005-01-01

    Full Text Available This paper describes the application of Value Stream Mapping to identify the waste in cement-package industry on PT IHSG. The Value Stream Mapping is used to map the activity towards the company's supply chain with the result that the non-value adding activity could be identified. The result will be an important fundament for identifying the defect and time. The effort for identifying the company's improvement meet the problem priority and it has a significant impact so that the time and cost consuming could be avoided. Abstract in Bahasa Indonesia : Paper ini menggambarkan bagaimana aplikasi pemetaan aliran nilai dapat mengidentifikasi waste pada industri kemasan semen. Pemetaan aliran nilai digunakan untuk memetakan aktivitas pada rantai pasok perusahaan sehingga aktivitas yang tidak bernilai tambah dapat diketahui. Hasil pemetaan akan menjadi landasan penting didalam mengetahui cacat dan waktu tunggu. Upaya untuk mengidentifikasi perbaikan perusahaan sesuai dengan masalah prioritas yang ada dan memiliki dampak yang signifikan sehingga tidak terjadi pemborosan biaya dan waktu program perbaikan. Kata kunci: pemetaan aliran nilai, industri kemasan semen, waste.

  7. Alkaline phosphatase in stallion semen: characterization and clinical applications.

    Science.gov (United States)

    Turner, R M O; McDonnell, S M

    2003-06-01

    Significant amounts of alkaline phosphatase (AP) activity have been found in semen plasma from numerous species. In species in which the majority of semen plasma AP (SPAP) activity originates from the epididymis and testicle, SPAP activity can be used clinically as a marker to differentiate testicular origin azoospermia or oligospermia from ejaculatory failure. Information on SPAP activity in stallions to date has been limited. In this study, a standard clinical chemistry analyzer was used to determine AP activity in pre-ejaculatory fluid and ejaculates from groups of normal stallions. Additionally, accessory glands, epididymides, testicles and other components of the urogenital tract of normal stallions were assayed to determine which tissues contain SPAP activity. The results indicated that levels of AP activity are low in pre-ejaculatory fluid, but significantly higher in ejaculatory fluid from normal stallions. Spermatozoa were not a significant source of SPAP activity. High levels of SPAP activity were found in the testes and epididymides. These findings suggest that SPAP activity is a candidate for a sperm-independent marker for ejaculation in the stallion. Finally, AP activity was determined in ejaculatory fluid from a stallion with bilaterally blocked ampullae, both before and after relief of the blockage. While the blockage was present, AP activity in ejaculatory fluid was low. However, following relief of the blockage, AP activity in ejaculatory fluid rose dramatically, thus suggesting that AP activity will be useful as an inexpensive, simple clinical assay for differentiating ejaculatory failure or excurrent duct blockages from testicular origin azoospermia and oligospermia.

  8. EFFECTS OF SELENOMETHIONINE AND VITAMIN C ON SEMEN.

    Directory of Open Access Journals (Sweden)

    Cristiane A Alvarez e Gentil Vanini de Moraes

    2006-06-01

    Full Text Available Spermatozoa are susceptible to peroxidative damages due to the high concentration of polyunsaturated fatty acids in its cytoplasmic membranes. Studies have shown that the spermatozoa and seminal leukocytes are capable to generate high levels of reactive oxygen species (ROSs that may reduce the viability and fertility of spermatozoa. However, small quantities of ROSs are necessary to initiation of the function of spermatozoa, as well as, capacitation and induction of the acrosomic reaction. Thus an equilibrium between the production of ROSs and antioxidative protection is necessary to assure the spermatic function. The antioxidative protection of the semen is supplied by enzymes such as superoxide dismutase, glutathione peroxidase (GPx, catalase, vitamin C, vitamin E and other substances (albumine, glutathione, taurine, hypotaurine found inside of spermatic cells or in seminal plasma. Thus, the objective of this revision is characterize how reactive oxygen species cause irreparable damages to spermatozoa membranes and the importance of the antioxidative protection of the semen that can be promoted by the addition of simple minerals like selenium and vitamins (e.g. ascorbic acid.

  9. Studies on Freezing RAM Semen in Absence of Glycerol.

    Science.gov (United States)

    Abdelnaby, Abdelhady Abdelhakeam

    1988-12-01

    Glycerol is widely used as a major cryoprotective agent for freezing spermatozoa of almost all species. However, it reduces fertility of sheep inseminated cervically compared with intrauterine insemination. Studies were conducted to develop a method and procedure for freezing ram semen in the absence of glycerol. Post -thaw survival of ram spermatozoa frozen in the absence of glycerol was affected by time and temperature after collection and before dilution and time after dilution and before freezing. Increase in time at 5^ circC before or after dilution and before freezing increased both post-thaw motility and number of cells passing through Sephadex filter. A cold dilution method was developed. Slow cooling of fresh ram semen and diluting at 5^circ C 2-3 hr. after collection, then freezing 1 hr. after dilution improved both post-thaw motility and number of cells passing through Sephadex filter compared with immediate dilution at 30-37^circC after collection and freezing 3-4 hr. later (P synchronization during breeding season resulted in higher heat response and lambing rate than two injections given 10 days apart.

  10. Semen quality and heavy metal and pesticides toxicity

    Directory of Open Access Journals (Sweden)

    Lidia Mínguez-Alarcón

    2014-06-01

    Full Text Available Male reproductive function has deteriorated significantly in the past 50 years and this change could be related to an exposure to occupational and environmental pollutants and toxicants. The purpose of this paper is to summarize the negative impact of human exposure to heavy metals and pesticides on the male reproductive function. Most pesticides and heavy metals are considered reproductive toxicants and may adversely harm the male reproductive system due to their disrupting effect on the hypothalamus- pituitary gland-gonads axis or by directly affecting spermatogenesis, resulting in impaired semen quality. The negative effects of these compounds have been linked to the main sperm parameters (concentration, normal morphology and motility, semen volume and total sperm count and DNA sperm damage, as well as to changes in serum reproductive hormone levels. Some of these substances have already been banned, whereas others are still on the market. Stricter laws are needed to completely prevent exposure to these toxicants given their proven deleterious effect on male reproductive health.

  11. A comparison of conventional and computer-assisted semen analysis (CRISMAS software) using samples from 166 young Danish men

    DEFF Research Database (Denmark)

    Vested, Anne; Ramlau-Hansen, Cecilia; Bonde, Jens P;

    2011-01-01

    The aim of the present study was to compare assessments of sperm concentration and sperm motility analysed by conventional semen analysis with those obtained by computer-assisted semen analysis (CASA) (Copenhagen Rigshospitalet Image House Sperm Motility Analysis System (CRISMAS) 4.6 software) us...... and motility analysis. This needs to be accounted for in clinics using this software and in studies of determinants of these semen characteristics.......The aim of the present study was to compare assessments of sperm concentration and sperm motility analysed by conventional semen analysis with those obtained by computer-assisted semen analysis (CASA) (Copenhagen Rigshospitalet Image House Sperm Motility Analysis System (CRISMAS) 4.6 software......) using semen samples from 166 young Danish men. The CRISMAS software identifies sperm concentration and classifies spermatozoa into three motility categories. To enable comparison of the two methods, the four motility stages obtained by conventional semen analysis were, based on their velocity...

  12. Congelación de semen de carnero en pellets con los diluyentes Tris-glucosa-yema de huevo o Lactosa-yema de huevo

    Directory of Open Access Journals (Sweden)

    F. I. Brito

    2004-01-01

    Full Text Available El objetivo del trabajo fue comparar la recuperación de la motilidad al descongelar el semen congelado en pellets utilizando el diluyente Tris glucosa-yema de huevo o Lactosa-yema de huevo. Se obtuvieron 30 eyaculados de nueve carneros por medio de una vagina artificial y se evaluó el volumen, la motilidad progresiva, la concentración y la morfología espermática. Cada eyaculado se dividió en dos partes, una de ellas se diluyó con Tris-glucosa yema de huevo y la otra con Lactosa yema de huevo, de manera que la dilución final contuviera 100 x 106 de espermatozoides en 0.1 ml. Los pellets se prepararon goteando 0.1 ml del semen diluido en perforaciones realizadas en hielo seco. Se evaluó la motilidad inicial, después de diluir y al descongelado. Se evaluó la motilidad de cinco pellets de cada eyaculado preparado con cada diluyente (300 en total. Los datos se analizaron estadísticamente mediante análisis de varianza, considerando la motilidad progresiva como variable dependiente y transformando al arcoseno los valores expresados en porcentaje. No se encontraron diferencias en la motilidad inicial de los eyaculados diluidos con tris o Lactosa (P>0.05. La motilidad espermática al descongelar fue significativamente mayor (P<0.05 en el semen diluido con Lactosa-yema (45.8%, que con Tris-glucosa-yema (40.2%

  13. Análisis microbiológico y caracterización de poblaciones bacterianas en sistemas de engorde de camarón durante un ciclo de cultivo

    OpenAIRE

    Cedeño, Ricardo

    2005-01-01

    Análisis microbiológico y caracterización de poblaciones bacterianas en sistemas de engorde de camarón durante un ciclo de cultivo En la literatura científica existen varios trabajos de caracterización de la microbiota bacteriana en sistemas de cultivo de Penaeidos, pero muy poca información con respecto a la especie Litopenaeus vannamei en sistemas de engorde locales.

  14. PROSTAGLANDIN F2α SUPPLEMENTED SEMEN IMPROVES LANDRACE BOARS SPERM MOTILITY

    Directory of Open Access Journals (Sweden)

    NICOLETA IONESCU

    2009-05-01

    Full Text Available This study investigated whether the sperm motility from Landrace boars improveswhen PGF2α (Dinolytic®; 5 mg PGF2α /ml was added to diluted semen. Boars fromone large production unit, were manually collected; semen was either enriched withPGF2α (group 1, n=38, either untreated (group 2, n=32. Total volume of semencollected, percent of motility and number of obtained doses were recorded. Thehighest sperm volume collected from the two groups is corresponding to ejaculatesfrom Landrace boars with PGF2α supplemented semen (267.6 ml. Regardingmotility, the sperm collected from Landrace boars with PGF2α supplemented semenwas higher from the one collected from Landrace boars with untreated semen(81.37% and very significant differences were statistically determined. Theejaculates with highest number of obtained doses is corresponding to the onescollected from boars with PGF2α supplemented semen (25.21. Only boars from thefirst group (with PGF2α supplemented semen showed motility over 70% and even100%. The untreated semen showed motility values around 65-70%.

  15. Dark-coloured semen in nonobstructive azoospermia: a report of four cases.

    Science.gov (United States)

    Halder, A; Jain, M; Chaudhary, I; Kumar, G; Das, T; Gupta, Y K

    2014-04-01

    Dark-coloured semen is rarely observed in men with nonobstructive azoospermia in absence of genital tract bleeding. Dark-coloured semen is also observed rarely in men with spinal cord injury with or without genital tract bleeding. However, this condition has not been reported in the literature in absence of genital tract bleeding or spinal cord injury. Here, we report men with nonobstructive azoospermia with dark-coloured semen and without genital tract bleeding or spinal cord injury. The study included four subjects with dark-coloured semen. All the samples first investigated for the presence of red blood cells as well as haemoglobin. All the cases were examined in details with seminal, hormonal and genetic parameters. In addition, an elemental electron microscopy and inductively coupled plasma-atomic emission spectrometry evaluation for heavy metals was performed. The semen parameters showed normal volume, pH, leucocyte count and azoospermia or oligoazoospermia. Dark-coloured semen specimens contained neither red blood cells nor haeme pigment. Electron microscopy showed presence of platinum in all the cases, whereas inductively coupled plasma-atomic emission spectrometry revealed increased levels of lead, manganese and nickel in serum as compared to controls. The results indicate dark-coloured semen may be linked with heavy metals found in seminal cells.

  16. Comparative evaluation of Nabi and Beltsville extenders for cryopreservation of rooster semen.

    Science.gov (United States)

    Nabi, Mohammad Mahdi; Kohram, Hamid; Zhandi, Mahdi; Mehrabani-Yeganeh, Hassan; Sharideh, Hossein; Zare-Shahaneh, Ahmad; Esmaili, Vahid

    2016-02-01

    Two experiments were conducted to evaluate the new rooster semen freezing extender which is containing a low level of glycerol and soybean lecithin as an alternative protective agent in the extender. The aim of the first experiment was to evaluate a new extender for freeze-thawing rooster semen known as "Nabi" extender compared to Beltsville. Second experiment was also performed to determine whether the Nabi extender has negative reactions on fertilization after artificial insemination (AI) or no. In the first experiment, post-thaw motion parameters, mitochondrial function and sperm apoptosis were analyzed using Sperm Class Analyzer (SCA), rhodamine-123 and Annexin-V, respectively for frozen-thawed semen in Nabi and Beltsville extender. Results showed that total motility, progressive motility, velocity parameters (VCL, VSL, VAP, LIN and STR) and live spermatozoa with active mitochondria were significantly higher in Nabi compare to Beltsville extender (P hens (Ross 308) were inseminated with thawed semen using the new freezing diluents or fresh semen for determination of fertility rate. Fertility rate with thawed semen (with Nabi extender) was lower compared to fresh semen (by approximately 8% points). It can be concluded that Nabi extender would improve post-thawed rooster sperm in vitro quality compared to Beltsville extender. The fertility rates of insemination in hens with freeze-thaw sperm were comparable with fresh sperm.

  17. Bacteriospermia and Its Impact on Basic Semen Parameters among Infertile Men

    Directory of Open Access Journals (Sweden)

    Sangeetha Vilvanathan

    2016-01-01

    Full Text Available Introduction. Semen analysis is considered as the surrogate marker for male fecundity while assessing infertile men. There are several reasons for altered semen quality and bacteriospermia could be one among them. Thereby the aim of our work is to study the semen culture and its impact on semen parameters among infertile men. Materials and Methods. Semen samples were collected from men attending infertility clinic. Semen parameters were analysed based on WHO guidelines. Also, samples were subjected to culture using standard bacteriological techniques. Results. A total of 85 samples were collected. A number of 47 (55.30% had normal sperm count, 37 (43.50% had oligozoospermia, and one (1.17% had azoospermia. Teratozoospermia was the most common abnormality observed (81.17% followed by asthenozoospermia (28.23%. The prevalence of bacteriospermia was 35.3%. Enterococcus faecalis (30% was the most common organism isolated followed by Coagulase negative Staphylococcus (23.33%, Staphylococcus aureus (20%, and E. coli (10%. Other less frequently isolated organisms were Klebsiella pneumoniae (6.66%, Proteus sp. (6.66%, and Citrobacter sp. (3.33%. Conclusion. The presence of asymptomatic bacteriospermia did not correlate with abnormal semen parameters.

  18. Bacteriospermia and Its Impact on Basic Semen Parameters among Infertile Men

    Science.gov (United States)

    Kandasamy, Balan; Jayachandran, Abiramy Lakshmy; Sathiyanarayanan, Sarasa; Tanjore Singaravelu, Vijayalakshmi; Krishnamurthy, Veeraraghavan; Elangovan, Vanithadevi

    2016-01-01

    Introduction. Semen analysis is considered as the surrogate marker for male fecundity while assessing infertile men. There are several reasons for altered semen quality and bacteriospermia could be one among them. Thereby the aim of our work is to study the semen culture and its impact on semen parameters among infertile men. Materials and Methods. Semen samples were collected from men attending infertility clinic. Semen parameters were analysed based on WHO guidelines. Also, samples were subjected to culture using standard bacteriological techniques. Results. A total of 85 samples were collected. A number of 47 (55.30%) had normal sperm count, 37 (43.50%) had oligozoospermia, and one (1.17%) had azoospermia. Teratozoospermia was the most common abnormality observed (81.17%) followed by asthenozoospermia (28.23%). The prevalence of bacteriospermia was 35.3%. Enterococcus faecalis (30%) was the most common organism isolated followed by Coagulase negative Staphylococcus (23.33%), Staphylococcus aureus (20%), and E. coli (10%). Other less frequently isolated organisms were Klebsiella pneumoniae (6.66%), Proteus sp. (6.66%), and Citrobacter sp. (3.33%). Conclusion. The presence of asymptomatic bacteriospermia did not correlate with abnormal semen parameters. PMID:26880908

  19. NEW HUMAN SEMEN ANALYSIS SYSTEM (CASA USING MICROSCOPIC IMAGE PROCESSING TECHNIQUES

    Directory of Open Access Journals (Sweden)

    N M Chaudhari

    2016-11-01

    Full Text Available Computer assisted semen analysis (CASA helps the pathologist or fertility specialist to evaluate the human semen. Detail analysis of spermatozoa like morphology and motility is very important in the process of intrauterine insemination (IUI or In-vitro fertilization (IVF in infertile couple. The main objective for this new semen analysis is to provide a low cost solution to the pathologist and gynecologist for the routine raw semen analysis, finding the concentration of the semen with dynamic background removal and classify the spermatozoa type (grade according to the motility and structural abnormality as per the WHO criteria. In this paper a new system , computer assisted semen analysis system is proposed in which hybrid approach is used to identify the moving object, scan line algorithm is applied for confirmation of the objects having tails, so that we can count the actual number of spermatozoa. For removal of background initially the dynamic background generation algorithm is proposed to create a background for background subtraction stage. The standard data set is created with 40× and 100× magnification from the different raw semen s. For testing the efficiency of proposed algorithm, same frames are applied to the existing algorithm. Another module of the system is focused on finding the motility and Type classification of individual spermatozoa.

  20. In vivo adverse effects of alpha-tocopherol on the semen quality of male bucks.

    Science.gov (United States)

    Majid, A; Qureshi, M S; Khan, R U

    2015-10-01

    Oxidative stress has detrimental effects on semen quality during spermatogenesis and semen processing for artificial insemination. This work was conducted to study the effect of different levels of vitamin E on the semen traits, oxidative status and trace minerals in Beetal bucks. Thirty-six bucks of similar body weight and age (1 year) were randomly divided into four groups. One group was kept as control with no supplementation (group 1), and the others were supplemented with 200 (group 2), 400 (group 3) and 800 IU (group 4) vitamin E/animal/day for 2 months. At the end of the experiment, semen samples were collected and evaluated. Seminal plasma was separated to study the concentration of superoxide dismutase (SOD), glutathione peroxidase (GPx), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and trace minerals (Zn, Cu, Mn and Fe). Group 3 showed significantly higher (p Zn, Cu and Mn were higher in the same group. The level of AST decreased in group 3 without any change on the concentration of ALT. It is suggested that vitamin E at the rate of 400 IU/buck/day supported higher semen volume, per cent motility, per cent live spermatozoa, antioxidants (SOD, GPx) and trace mineral levels (Zn, Cu, Mn) in the seminal plasma. The increased supplementation from 0 to 400 showed a general increasing trend in improving semen quality. However, the dose of 800 IU/kg had no useful effect in further improving the semen quality.

  1. Impact of collection method on assessment of semen HIV RNA viral load.

    Directory of Open Access Journals (Sweden)

    Brendan J W Osborne

    Full Text Available BACKGROUND: The blood HIV RNA viral load is the best-defined predictor of HIV transmission, in part due to ease of measurement and the correlation of blood and genital tract (semen or cervico-vaginal viral load, although recent studies found semen HIV RNA concentration to be a stronger predictor of HIV transmission. There is currently no standardized method for semen collection when measuring HIV RNA concentration. Therefore, we compared two collection techniques in order to study of the impact of antiretroviral therapy on the semen viral load. METHODOLOGY/PRINCIPAL FINDINGS: Semen was collected by masturbation from HIV-infected, therapy-naïve men who have sex with men (MSM either undiluted (Visit 1 or directly into transport medium (Visit 2. Seminal plasma was then isolated, and the HIV RNA concentration obtained with each collection technique was measured and corrected for dilution if necessary. Collection of semen directly into transport medium resulted in a median HIV RNA viral load that was 0.4 log10 higher than undiluted samples. CONCLUSIONS/SIGNIFICANCE: The method of semen collection is an important consideration when quantifying the HIV RNA viral load in this compartment.

  2. Season-induced variation in lipid composition is associated with semen quality in Holstein bulls.

    Science.gov (United States)

    Argov-Argaman, N; Mahgrefthe, K; Zeron, Y; Roth, Z

    2013-05-01

    Season-induced variation in fatty acid and cholesterol composition in bovine semen has been associated with semen quality. Given the specific roles of the various semen compartments (seminal fluids, sperm head, and sperm tail) in fertilization, we hypothesized that environmental-stress-induced alterations in the lipid composition of a specific compartment might impair semen quality and sperm function. Semen samples were collected from five mature Holstein-Friesian bulls during the summer (August to September) and winter (December to January). Semen was evaluated by computerized sperm-quality analyzer, calibrated for bulls' semen, and centrifuged to separate the spermatozoa from the seminal fluids. The spermatozoal fraction was sonicated to separate the sperm head and tail compartments. Cold lipid extraction was performed with chloroform:methanol (2:1, vol/vol). Lipids were identified and quantified by gas chromatography. Seasonal variation was found in both physiological and structural parameters. The proportion of spermatozoa defined as morphologically normal was higher in the winter, with higher motility, progressive motility, and velocity relative to summer samples. Lipid composition within fractions varied between seasons with prominent impairment in the tail compartment, characterized by high saturated fatty acid, low polyunsaturated fatty acid, and low cholesterol concentrations during the summer. Given the association between alterations in lipid composition and reduced sperm motility and velocity during the summer, it is suggested that lipid composition might serve to predict sperm quality.

  3. Does exposure to computers affect the routine parameters of semen quality?

    Institute of Scientific and Technical Information of China (English)

    Yue-Lian Sun; Wei-Jin Zhou; Jun-Qing Wu; Er-Sheng Gao

    2005-01-01

    Aim: To assess whether exposure to computers harms the semen quality of healthy young men. Methods: A total of 178 subjects were recruited from two maternity and children healthcare centers in Shanghai, 91 with a history of exposure to computers (I.e., exposure for 20 h or more per week in the last 2 years) and 87 persons to act as control (no or little exposure to computers). Data on the history of exposure to computers and other characteristics were obtained by means of a structured questionnaire interview. Semen samples were collected by masturbation in the place where the semen samples were analyzed. Results: No differences in the distribution of the semen parameters (semen volume, sperm density, percentage of progressive sperm, sperm viability and percentage of normal form sperm) were found between the exposed group and the control group. Exposure to computers was not found to be a risk factor for inferior semen quality after adjusting for potential confounders, including abstinence days, testicle size, occupation, history of exposure to toxic substances. Conclusion: The present study did not find that healthy men exposed to computers had inferior semen quality.

  4. POLARIDAD ESTRUCTURAL Y DINAMICA DE POLIMERIZACION DE LA TUBULINA BACTERIANA BTUBA/B DE PROSTHECOBACTER DEJONGEII

    OpenAIRE

    DIAZ CELIS, CESAR DAVID

    2010-01-01

    El heterodímero de α/β-tubulina , la proteína que forma el citoesqueleto de microtúbulos, está presente en todos los eucariontes conocidos, pero nunca ha sido detectado en bacterias o arqueas. Por este motivo, se consideró a la tubulina como una proteína típica del citoesqueleto de los eucariontes. Sin embargo, en el año 2002 se descubrió en el genoma de cuatro especies del género bacteriano Prosthecobacter (división Verrucomicrobia) dos nuevas secuencias de tubulina, btubA y btubB ("bacte...

  5. Ureaplasma Urealyticum or Mycoplasma Hominis Infections and Semen Quality of Infertile Men in Abidjan

    Institute of Scientific and Technical Information of China (English)

    Zinzendorf NY; Kouassi-Agbessi BT; Lathro JS; Don C; Kouadio L; Loukou YG

    2008-01-01

    Objective To determine the prevalence of U.urealvticum and M.hominis in semen samples collected from men admitted in clinic for infertility,and to compare the quality of these semen samples.Methods A total of 1058 semen samples collected were investigated.Sperm semiological assays were performed according to the guidelines of the World Health Organisation(WHO).Semen were examined by Mycoplasma IST for the detection of mycoplasma.Semen culture on agar media was used to detect other microorganisms.Chlamydia was detected using direct fluorescent assay(DFA)of Clamydia Trachomatis.Results Among 1058 semen samples,microorganisms were detected in 638(60.3%).The infected sperms consisted of mycoplasma alone in 507 cases(47.9%).mycoplasma and other microorganisms in 98(9.3%),giving in all 605(57.2%)samples infected with mycoplasma.The last 33(3.1%)consisted of other microorganisms alone.The frequency of U.urealyticum.M.hominis and mixed genital infections detected in semen samples of infertile men were 39%,23.8% and 5.6%,respectively.The rates of abnormal semen parameters recorded among patients infected with mycoplasma were for volume(22.2%-25%),viscosity(29.6%-43.5%),pH(64.7%-72.9%),motility(80.8%-93.8%),morphology(36.3%-47.9%),sperm concentration(53.3%-58.3%)and leukocyte count(51.4%-58.3%).Conclusion Frequency of U.urealyticum infection was higher than that of M.hominis.Mycoplasma infections were associated with disorders of pH,motility and sperm concentration.In addition M.hominis infection affected spermatozoa morphology.Therefore,screening of U.urealyticum and M.hominis for routine semen analysis is clinically relevant in Abidjan.

  6. Effect of preputial washing on bacterial load and preservability of semen in Murrah buffalo bulls

    Directory of Open Access Journals (Sweden)

    G. S. Meena

    2015-06-01

    Full Text Available Aim: To study the effect of preputial washing on bacterial load, preservability and semen quality in Murrah buffalo bulls Materials and Methods: A total of 36 collections of three Murrah buffalo bulls maintained at Artificial Breeding Research Centre, ICAR-National Dairy Research Institute, Karnal, were collected at weekly intervals from each bull without preputial washing and latter ejaculates from same bull with preputial washing by infusing normal saline (0.85%, KMnO4 (0.02% and savlon (2.0% to first, second and third bull, respectively. The microbial load and semen quality were evaluated during different hours of storage at refrigerated temperature (0, 24 and 48 h and after thrawing of cryopreserved (at −196°C semen. Results: The results of preservation of semen at refrigerated temperature showed that bacterial load was markedly lower in ejaculates of bulls subjected to preputial washing. Semen preserved at refrigerator temperature and cryopreserved, the effect of washing solution was significant for individual motility (IM, non-eosiniphilic count, hypo-osmotic swelling reactivity (HOST, total plate count (TPC and acrosome integrity. KMnO4 was found to be the best in lowering bacterial load, sperm abnormalities and in improving semen quality such as motility, non-eosinophilic count, HOST and acrosome integrity even up to 48 h of preservation and cryopreserved semen. Effect of duration of preservation and stage of cryopreservation was also significant for IM, non-eosiniphilic count, HOST, sperm abnormalities and acrosome integrity. Conclusion: Overall the results suggested that preputial washing with KMnO4 solution improved the semen quality and reduced microbial load of Murrah buffalo bull’s semen preserved at refrigerated temperature and cryopreservation.

  7. Kualitas Semen Beku Kuda pada Pengencer Susu Skim dengan Konsentrasi Gliserol yang Berbeda

    Directory of Open Access Journals (Sweden)

    Azizah -

    2009-06-01

    Full Text Available Cryoprotectant is one of determining factors for the success of stallion semen cryopreservation. Formost mammalian sperm cryopreservation,glycerol has been widely used as the cryoprotectant. Glycerol inhigh concentration is toxic to the sperm but in low concentration it has protective effect on the semen. Theobjectives of this experiment were to find appropiate glycerol concentration by using skim milk extender inorder to maintain motility and viability of spermatozoa of stallion frozen semen. Semen was collectedfrom three sexually mature stallions by using artificial vagina. The semen characteristics and qualitywere examined both macro and microscopically. Prior to extension, semen was centrifugated at 3000 rpmfor 15 minutes. The pellet was re-extended with skim milk extender with glycerol 5% (G5 7.5% (G7.5, and10% (G10. The extended semen as then packed in mini straw (0.3ml, equilibrated at 5 oC for 2 hours,frozen in the liquid N2 vapor for15 minutes and then stored in liquid N2 container until futher evaluation.The frozen semen, was thawed at 37 oC for 30 seconds. The percentages of sperm motility and viabilitywere examihed. The result of this research showed that the sperm motility and viability in G5 was (23.8 %;35.6 % significantly hihger (P<0.05 as compare a with G7.5 (18.1%; 28.6% and G10. (11.8%; 23.1%. Therecovery Rate in G5 (35.4% was significant high er (P<0.05 than G7.5 (26.9% and G10 (17.6%. In conclusion,glicerol of 5% is recomended for cryopreservation of stallion semen extended with skim milk.

  8. Semen evaluation in four autochthonous wild raptor species using computer-aided sperm analyzer.

    Science.gov (United States)

    Dogliero, Andrea; Rota, Ada; Lofiego, Renato; Mauthe von Degerfeld, Mitzy; Quaranta, Giuseppe

    2016-04-01

    At least 10 percent of the approximately 300 species of the order Falconiformes are listed as being globally threatened. The present work describes the seminal characteristics of three diurnal and one nocturnal raptor species. Semen was collected from clinically healthy Accipiter nisus (n = 1), Falco subbuteo (n = 6), and Falco tinnunculus (n = 5) adult males that were housed at the 'Centro Animali Non Convenzionali' of the Department of Veterinary Sciences of the University of Turin. The semen was collected after a period of recovery and before their release as well as from seven Bubo bubo males bred in captivity as part of a raptor conservation project. All the potential semen donors were trained in semen collection during the breeding season via a ritualized procedure. Ejaculation was achieved using a massaging technique. Each sample was evaluated for volume, degree of contamination, and spermatozoa concentration. The semen motility and kinetic parameters were assessed on diluted semen (modified tyroides albumin lactate pyruvate, pH 7.5, temperature 37.5 °C) using a computer-aided sperm analyzer. Semen collection was successful in all the diurnal species and in five B bubo individuals. The sperm motility and sperm kinetic parameters were very variable both among and within species. In contrast with previous studies that involved raptors bred in captivity and imprinted on humans, we worked with wild birds and attempted to overcome the problem of poor semen quality, which is strongly influenced by stress, by adopting a ritualized procedure that has never been reported for semen collection purposes.

  9. Effect of age and abstinence on semen quality:A retrospective study in a teaching hospital

    Institute of Scientific and Technical Information of China (English)

    Priyadarsini Sunanda; Babita Panda; Chidananda Dash; Rabindra N Padhy; Padmanav Routray

    2014-01-01

    Objective: To elucidate the effect of age and sexual abstinence on semen quality (semen volume, total count, progressive motility, vitality and morphology). Methods:A total of 730 semen samples were analyzed. Subjects were grouped according to the age (20-29, 30-34, 35-39 and 40-50) and abstinence (2-3, 4-5 and 6-7). Semen parameters were evaluated following WHO standard criteria. Results: Analysis of 730 semen samples showed negative correlation of progressive motility (r=-0.131, P< 0.01), vitality (r=-0.173, P< 0.01), morphology (r=-0.324, P< 0.01) with age. With increase in age percentage of progressive motility, vitality and normal morphology in mean values declined after the age group of 35-39 to 40-50 years, but no change in volume and count were observed. Increase in abstinence with individual days significantly affected semen volume (H=20.65, P<0.001), count (H=36.67, P<0.01), progressive motility (H=13.53, P<0.05) and vitality (H=15.33, P<0.01). But, no effect was found on sperm morphology. Mann Whitney U test confirmed the changes in semen volume, total count and vitality in paired grouping from 2-7 days (P<0.05), but changes in sperm motility were observed after 5 days of abstinence in each paired group upto 7 days (P<0.05). Mean values of semen parameters among three abstinence groups (2-3, 4-5 and 6-7 days) also showed similar result. Conclusions:In the present study, age negatively affected progressive motility, vitality and morphology of human sperm. Semen samples showed intra varied results within WHO amended abstinence period.

  10. The reference values for semen parameters of 1213 fertile men in Guangdong Province in China

    Directory of Open Access Journals (Sweden)

    Yun-Ge Tang

    2015-04-01

    Full Text Available Semen samples were collected from 1213 fertile men whose partners had a time-to-pregnancy (TTP ≤12 months in Guangdong Province in Southern China, and semen parameters including semen volume, sperm concentration, total counts, motility, and morphology were evaluated according to the World Health Organization (WHO 2010 guideline. All semen parameters analyzed were normal in ~62.2% of the total samples, whereas ~37.8% showed at least one of the semen parameters below normal threshold values. The fifth centiles (with 95% confidence intervals were 1.3 (1.2-1.5 ml for semen volume, 20 × 10 6 (18×10 6 -20×10 6 ml−1 for sperm concentration, 40 × 10 6 (38×10 6 -44×10 6 per ejaculate for total sperm counts, 48% (47%-53% for vitality, 39% (36%-43% for total motility, 25% (23%-27% for sperm progressive motility, 5.0% (4%-5% for normal morphology. The pH values ranged from 7.2 to 8.0 with the mean ± standard deviation at 7.32 ± 0.17. No effects of age and body mass index were found on semen parameters. Occupation, smoking and alcohol abuse, varicocele appeared to decrease semen quality. Sperm concentration, but not sperm morphology, is positively correlated with TTP, whereas vitality is negatively correlated with TTP. Our study provides the latest reference values for the semen parameters of Chinese fertile men in Guangdong Province, which are close to those described in the new WHO guidelines (5 th Edition.

  11. Improvement of the Shami goat semen quality by adding bovine serum albumin

    Directory of Open Access Journals (Sweden)

    O.I. Azawi

    2014-06-01

    Full Text Available The present study was aimed to improve the quality of Shami goat semen diluted with Tris diluent by adding bovine serum albumin. In the current study, six male goats were used. Semen was collected using artificial vagina of one ejaculate per week of every male included in this study. This study was performed during the breeding season from 1 \\ 10 \\ 2012 to 1 \\ 12 \\ 2012. In this study, two semen diluents were use first; Tris- fructose- egg yolk 2.5% and second Tris - fructose - 2.5% egg yolk with 1% of bovine serum albumin. Diluted semen samples were cooled gradually and stored at 5 ° C. Cooled diluted semen samples were examined every 24 h of storage to 144 h. These tests includes the proportion of live sperm and the percentage of secondary abnormalities of the sperm, the percentage of sperm acrosomal defects and percentage of progressive motility using a computer-aided sperm analysis. These results showed that the addition of bovine serum albumin with egg yolk to semen of male goats led to improved qualities of semen significantly (P<0.05 including the proportion of live sperm and the percentage of secondary abnormalities of the sperm, the percentage of sperm acrosomal defects and percentage of progressive motility. It could be concluded from the results of the current study, the possibility of storing goat semen for more than six days with alive sperm of more than 50% and the percentage of the progressive motility of more than 40% when adding bovine albumin serum to dilute goat semen at 1% level and this result has not reached by any previous study.

  12. Effect of organic selenium on turkey semen quality during liquid storage.

    Science.gov (United States)

    Dimitrov, S G; Atanasov, V K; Surai, P F; Denev, S A

    2007-08-01

    The aim of the present study was to evaluate the effect of dietary organic selenium on the turkey semen during storage. Twenty males (BUT, Big 6, 40 weeks of age) were divided into control (n=10) and experimental group (n=10). The turkeys in the both groups were fed with a commercial diet containing 0.1 ppm Se in the form of sodium selenite. The experimental birds were additionally supplied with 0.3 ppm organic Se in the form Sel-Plex (Alltech, Inc.). After 30 days of feeding, the semen samples were collected twice a week for the 3 weeks of the study and diluted 1+1(v/v) with TUR-2 diluent, and stored in a water bath (+10 to 15 degrees C) for 6 h. The percentage of motile spermatozoa, the sperm viability (live/dead spermatozoa), total lipids, phospholipids and total cholesterol were assessed in fresh and stored semen. The fertilizing ability of semen was assessed by artificial insemination of 30 hens per group with dose containing 200x10(6) spermatozoa weekly. After 6 h of semen storage, the motility of spermatozoa decreased significantly in the control group (by 8.7 relative percent, P0.05) in experimental group reflecting a protective effect of dietary Se supplementation. The proportion of live spermatozoa was higher in fresh semen and significantly lower in stored semen. The positive effect of Se supplementation was observed on the lipid composition of stored semen: the concentration of the total lipids and phospholipids in the seminal plasma from control group significantly increased, while in the experimental group remained constant. Better semen integrity in the experimental group was associated with an improved fertilizing ability of spermatozoa: the fertility rate of stored spermatozoa in the control group was 88%, while in the experimental group was 90.5%.

  13. Semen coagulum liquefaction, sperm activation and cryopreservation of capuchin monkey (Cebus apella) semen in coconut water solution (CWS) and TES-TRIS.

    Science.gov (United States)

    Oliveira, Karol G; Miranda, Stefania A; Leão, Danuza L; Brito, Adriel B; Santos, Regiane R; Domingues, Sheyla F S

    2011-01-01

    The objectives of the present study were to test the effect of coconut water solution and TES-TRIS on the seminal coagulum liquefaction, sperm activation in fresh diluted semen, and on the cryopreservation of semen from capuchin monkeys (Cebus apella). Semen was collected from six males by electro-ejaculation, diluted in TES-TRIS or coconut water solution (CWS), and incubated at 35°C until the coagulated fraction of the semen was completely liquefied. In the experiment I, after liquefaction, samples were diluted in TES-TRIS or CWS, plus 6 and 10mM/mL of caffeine. Sperm motility and vigor were evaluated during 5h. For experiment II, after liquefaction, semen samples were extended in TES-TRIS (3.5% glycerol in the final solution) or CWS (2.5% glycerol in the final solution), cryopreserved and stored in liquid nitrogen for 1 week. The seminal coagulum was liquefied in (mean±SDM) 4.5±1.7 and 2.8±1.1h in TES-TRIS and CWS, respectively. Sperm were motile in TES-TRIS and CWS for 5.0±1.4 and 1.0±0.5h, respectively. The mean motility in this period was 38±22% (TES-TRIS) and 22.0±16.0 (CWS). Motility increased after caffeine addition only in samples diluted in CWS containing 6mM (22.5±16.0) or 10mM (28.0±19.0) caffeine. Post-thaw live sperm percentage was 26.2% in TES-TRIS and 13.2% in CWS. For cryopreservation of semen from C. apella TES-TRIS (3.5% glycerol) was more appropriate than CWS (2.5% glycerol). CWS+caffeine potentially increase sperm motility and may be useful in artificial insemination of fresh diluted semen.

  14. Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis and Mycoplasma genitalium infections and semen quality of infertile men

    Directory of Open Access Journals (Sweden)

    Rebai Tarek

    2007-11-01

    Full Text Available Abstract Background Genital ureaplasmas (Ureaplasma urealyticum and Ureaplasma parvum and mycoplasmas (Mycoplasma genitalium and Mycoplasma hominis are potentially pathogenic species playing an etiologic role in both genital infections and male infertility. Reports are, however, controversial regarding the effects of these microorganisms infections in the sperm seminological variables. This study aimed at determining the frequency of genital ureplasmas and mycoplasmas in semen specimens collected from infertile men, and at comparing the seminological variables of semen from infected and non-infected men with these microorganisms. Methods A total of 120 semen samples collected from infertile men were investigated. Semen specimens were examined by in-house PCR-microtiter plate hybridization assay for the presence of genital ureaplasmas and mycoplasmas DNA. Semen analysis was assessed according to the guidelines of the World Health Organization. Standard parametric techniques (t-tests and nonparametric techniques (Wilcoxon tests were used for statistical analysis. Results The frequency of genital ureaplasmas and mycoplasmas detected in semen samples of infertile men was respectively 19.2% (23/120 and 15.8% (19/120. The frequency of Ureaplasma urealyticum (15% was higher than that of Mycoplasma hominis (10.8%, Ureaplasma parvum (4.2% and Mycoplasma genitalium (5%. Mixed species of mycoplasmas and ureaplasmas were detected in 6.7% of semen samples. Comparison of the parameters of the standard semen analysis between the male partners of the infertile couples with and without genital ureaplasmas and mycoplasmas infection showed that the presence of Mycoplasma hominis DNA in semen samples is associated with low sperm concentration (p = 0.007 and abnormal sperm morphology (p = 0.03 and a negative correlation between sperm concentration and the detection of Mycoplasma genitalium in semen samples of infertile men (p = 0.05. The mean values of seminal

  15. Use of immobilized cryopreserved bovine semen in a blind artificial insemination trial.

    Science.gov (United States)

    Standerholen, Fride Berg; Waterhouse, Karin Elisabeth; Larsgard, Anne Guro; Garmo, Randi Therese; Myromslien, Frøydis Deinboll; Sunde, Jan; Ropstad, Erik; Klinkenberg, Geir; Kommisrud, Elisabeth

    2015-08-01

    To make timing of artificial insemination (AI) relative to ovulation less critical, methods for prolonging shelf life of spermatozoa in vivo after AI have been attempted to be developed. Encapsulation of sperm cells is a documented technology, and recently, a technology in which sperm cells are embedded in alginate gel has been introduced and commercialized. In this study, standard processed semen with the Biladyl extender (control) was compared with semen processed by sperm immobilization technology developed by SpermVital AS in a blind field trial. Moreover, in vitro acrosome and plasma membrane integrity was assessed and compared with AI fertility data for possible correlation. Semen from 16 Norwegian Red young bulls with unknown fertility was collected and processed after splitting the semen in two aliquots. These aliquots were processed with the standard Biladyl extender or the SpermVital extender to a final number of 12 × 10(6) and 25 × 10(6) spermatozoa/dose, respectively. In total, 2000 semen doses were produced from each bull, divided equally by treatment. Artificial insemination doses were set up to design a blinded AI regime; 5 + 5 straws from each extender within ejaculates in ten-straw goblets were distributed to AI technicians and veterinarians all over Norway. Outcomes of the inseminations were measured as 56-day nonreturn rate (NRR). Postthaw sperm quality was assessed by flow cytometry using propidium iodide and Alexa 488-conjugated peanut agglutinin to assess the proportion of plasma membrane and acrosome-intact sperm cells, respectively. In total, data from 14,125 first inseminations performed over a 12-month period, 7081 with Biladyl and 7044 with SpermVital semen, were used in the statistical analyses. There was no significant difference in 56-day NRR for the two semen categories, overall NRR being 72.5% and 72.7% for Biladyl and SpermVital, respectively. The flow cytometric results revealed a significant higher level of acrosome-intact live

  16. Effect of vaginal douching and different semen extenders on bacterial load and fertility in turkeys.

    Science.gov (United States)

    Omprakash, A V; Venkatesh, G

    2006-08-01

    1. A study on artificial insemination of Beltsville Small White turkeys investigated the effect on bacterial load and fertility of vaginal douching with diluents containing Gentamicin 400 microg/ml and different semen extenders. 2. Irrespective of the extenders used, vaginal douching with Gentamicin reduced the microbial load of the vagina with resultant improvement in fertility and hatchability and corresponding reduction in embryonic mortality. 3. Eggs from hens inseminated with semen extended with Beltsville Poultry Semen Extender (BPSE) diluent along with vaginal douching showed a trend towards higher per cent fertility and per cent hatchability of total and fertile eggs set compared to other extenders, though this was non-significant.

  17. Content of heavy metals in the semen of bulls from various environments

    Energy Technology Data Exchange (ETDEWEB)

    Monkiewicz, J.; Jaczewski, S.; Dynarowicz, I.

    1975-01-01

    The content of metal ions (Cu, Pb, Zn) was examined in the semen of bulls kept under different environments. It was found that the content of Cu and Pb ions in the semen of bulls living near the copperworks and near to a highway was higher than that in the semen of bulls kept under normal conditions. The differences appeared to be highly significant statistically (P < 0.001). No significant statistical differences were found in the content of Zn. In addition, it was stated that the higher content of Pb influenced the survival of spermatozoons at highers temperature (46.5/sup 0/C). Further investigations will be performed under model conditions.

  18. Flora intermediária em mulheres em idade reprodutiva: aspectos inflamatórios, atividade de sialidases e carga bacteriana

    OpenAIRE

    Greatti, Mariana Morena de Vieira Santos [UNESP

    2014-01-01

    Espécies de lactobacilos são os principais componentes da microbiota vaginal e a manutenção do predomínio lactobacilar é importante para proteção desse ambiente contra possíveis patógenos. A vaginose bacteriana é uma condição em que se observa a perda de lactobacilos e substituição desses microrganismos por espécies bacterianas, anaeróbias em sua maioria. Tal condição pode acarretar inúmeras complicações ginecológicas e obstétricas, como o aumento do risco de aquisição de infecções sexualment...

  19. Perfil etiológico das meningites bacterianas em crianças Etiological profile of bacterial meningitis in children

    Directory of Open Access Journals (Sweden)

    Orlando C. Mantese

    2002-12-01

    Full Text Available Objetivo: analisar o perfil etiológico e alguns aspectos epidemiológicos das crianças com meningite bacteriana, internadas em um hospital público universitário. Métodos: foram seguidas, prospectivamente, as crianças internadas com meningite bacteriana, diagnosticada segundo os critérios clínicos e laboratoriais habituais. Foram excluídos os casos de meningite pós-trauma, de meningite na vigência de derivação liquórica, ou de defeitos congênitos do tubo neural, e de meningite tuberculosa. Resultados: foram analisadas 415 crianças internadas com diagnóstico de meningite bacteriana, no Hospital de Clínicas da Universidade Federal de Uberlândia, no período de 01/01/1987 a 31/01/2001. O agente etiológico foi identificado em 315 pacientes (75,9%, sendo de modo definitivo em 289 (69,3% e presuntivo, por intermédio da bacterioscopia, em outros 26 (6,6%. Os agentes mais comumente identificados foram o Haemophilus influenzae b (54,2%, o meningococo (20,6% e o pneumococo (18,1% dos 315 pacientes. O tratamento antimicrobiano prévio, detectado em 47,2% dos casos, causou uma diminuição significante no rendimento das culturas de sangue (de 50,8% para 38,7% e de liquor (71,7% para 57,6%. Houve um predomínio do acometimento de crianças com idade até 48 meses pelo Haemophilus influenzae b, particularmente em relação ao meningococo. A letalidade geral foi de 10,1%, com diferença significante entre a letalidade do pneumococo, de 17,5%, e a do meningococo, de 4,6%. Conclusões: as crianças afetadas por Haemophilus influenzae b e por pneumococo foram mais jovens que aquelas com meningite por meningococo. A hemocultura e a cultura de liquor continuam sendo importantes recursos laboratoriais para o diagnóstico etiológico da meningite bacteriana, apesar do impacto negativo causado em seu rendimento pelo tratamento antibiótico prévio. Os agentes mais comumente isolados foram o Haemophilus influenzae b, o meningococo e o pneumococo. A

  20. La resistencia bacteriana transmitida al hombre a través de los alimentos y el uso responsable de los antimicrobianos como moduladores de crecimiento

    OpenAIRE

    Gimeno, Emilio J.

    2001-01-01

    El objetivo de la presente comunicación es hacer un aporte para esclarecer a la opinión pública sobre la teoría y práctica de la resistencia bacteriana en relación con los moduladores de crecimiento, aportando datos, informes e ideas basadas en aspectos técnicos y hechos prácticos , científicamente demostrados sobre información consolidada.

  1. SEASONAL INFLUENCE ON SEMINAL PATTERN AND FREEZABILITY OF PANTANEIRO BULL SEMEN

    Directory of Open Access Journals (Sweden)

    Nathalia Hack Moreira

    2016-01-01

    Full Text Available The purpose of this paper was to investigate the effect of the season of the year on testicular morphology, fresh and frozen/thawed semen quality from Pantaneiro bulls bred in Brasilia, DF. Six bulls were submitted, once a month for one year, to evaluation of testicular measurements, semen collection using an artificial vagina and cryopreserved with medium containing Tris-based, egg  yolk and glycerol. The testicular length and volume were greater (P 0.05. In conclusion, Pantaneiro bulls, bred in the Midwest region of Brazil, can be used in natural mating programs in all seasons. Aiming to improve the Brazilian Animal Germplasm Bank with a higher number of samples collected, we suggest that the freezing of the semen is done between June and August, although there will be no losses of semen quality at any time. Keywords: bovine; conservation; cryopreservation; reproduction; seasonality.

  2. Semen characteristics and diabetes mellitus: significance of insulin in male infertility.

    Science.gov (United States)

    García-Díez, L C; Corrales Hernandez, J J; Hernandez-Diaz, J; Pedraz, M J; Miralles, J M

    1991-01-01

    A study was made of semen quality and serum hormonal profiles (FSH, LH, prolactin, testosterone) of patients with type I diabetes mellitus. Semen parameters and levels of prolactin and testosterone were significantly altered in the diabetic state. The concentration of insulin in serum and seminal plasma and the serum levels of FSH, LH, and testosterone were measured in 80 men classified in the following groups: fertile subjects, infertile normoglycemic subjects, subjects with carbohydrate intolerance, and excretory and secretory azoospermic subjects. In all groups, seminal insulin concentrations were higher than those obtained in serum. The hormone appears to freely cross the blood-testis barrier, there to be concentrated in the semen. The levels of insulin in serum and seminal plasma did not correlate with semen parameters and are not suitable markers of seminal quality. For unknown reasons, the concentrations of insulin in seminal plasma were lower in the subjects suffering from carbohydrate intolerance.

  3. Is semen a useful diagnostic tool for rare infections of the central nervous system?

    Directory of Open Access Journals (Sweden)

    Siddiqui Ruqaiyyah

    2012-12-01

    Full Text Available Abstract Given that the blood-testis barrier is more permeable than the blood–brain barrier, the use of semen to detect rare parasitic antigens/infections of the CNS in males is hypothesized.

  4. A novel method for semen collection and artificial insemination in large parrots (Psittaciformes).

    Science.gov (United States)

    Lierz, Michael; Reinschmidt, Matthias; Müller, Heiner; Wink, Michael; Neumann, Daniel

    2013-01-01

    The paper described a novel technique for semen collection in large psittacines (patent pending), a procedure which was not routinely possible before. For the first time, a large set of semen samples is now available for analysis as well as for artificial insemination. Semen samples of more than 100 psittacine taxa were collected and analysed; data demonstrate large differences in the spermatological parameters between families, indicating an ecological relationship with breeding behaviour (polygamous versus monogamous birds). Using semen samples for artificial insemination resulted in the production of offspring in various families, such as Macaws and Cockatoos, for the first time ever. The present technique represents a breakthrough in species conservation programs and will enable future research into the ecology and environmental factors influencing endangered species.

  5. Effect of Air Space in Storage Vials on Motility of Spermatozoa in Chilled Buck Semen

    Directory of Open Access Journals (Sweden)

    Magnus Paul K and Lali F Anand 1

    Full Text Available This study was conducted in order to find out the effect of air space on the top of glass vial in which semen is stored, on the motility of spermatozoa. 45 samples collected from two bucks over a span of 6 months were used for experiment. Goat milk extender was the diluent used. Two ml each of diluted semen after noting their initial motility was stored in 2 ml and 5 ml vials. Samples were stored at 5°C and motility of spermatozoa noted at 24 and 48 hours. Semen without air space was found to preserve the motility better than semen with air space on 24 and 48 hours of incubation. This could be better attributed to reactive oxygen species production by the spermatozoa, but further investigation is needed in this aspect to confirm it. [Veterinary World 2010; 3(9.000: 421-423

  6. Exposure to perfluorinated compounds and human semen quality in arctic and European populations

    DEFF Research Database (Denmark)

    Toft, Gunnar; Jönsson, B A G; Lindh, C H;

    2012-01-01

    Perfluorinated compounds (PFCs) have been suspected to adversely affect human reproductive health. The aim of this study was to investigate the associations between PFC exposure and male semen quality....

  7. Associations between dietary patterns and semen quality in men undergoing IVF/ICSI treatment

    NARCIS (Netherlands)

    Vujkovic, M.; Vries, de J.H.M.; Dohle, G.R.; Bonsel, G.J.; Lindemans, J.; Macklon, N.S.; Spek, van der P.J.; Steegers, E.A.P.; Steegers-Theunissen, R.P.M.

    2009-01-01

    BACKGROUND: This study investigates whether dietary patterns, substantiated by biomarkers, are associated with semen quality. METHODS: In 161 men of subfertile couples undergoing in vitro fertilization treatment in a tertiary referral clinic in Rotterdam, the Netherlands, we assessed nutrient intake

  8. Environmental mercury exposure, semen quality and reproductive hormones in Greenlandic Inuit and European men

    DEFF Research Database (Denmark)

    Mocevic, Emina; Specht, Ina O; Marott, Jacob Louis;

    2013-01-01

    Several animal studies indicate that mercury is a male reproductive toxicant, but human studies are few and contradictory. We examined semen characteristics and serum levels of reproductive hormones in relation to environmental exposure to mercury. Blood and semen samples were collected from 529.......024 to 0.110). The association may be due to beneficial effects of polyunsaturated fatty acids (PUFAs), which are contained in seafood and fish. No significant association (P>0.05) was found between blood concentrations of mercury and any of the other measured semen characteristics (semen volume, total...... sperm count, sperm concentration, morphology and motility) and reproductive hormones (free androgen index (FAI), follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone and LH×testosterone) in any region. In conclusion, the findings do not provide evidence that environmental mercury...

  9. Effect of smoking on reproductive hormones and semen parameters of infertile Saudi Arabians

    Directory of Open Access Journals (Sweden)

    Haifa A Al-Turki

    2015-01-01

    Conclusions: This study shows that the effect of smoking is dramatic reduction in the hormonal levels and semen parameters. It is recommended that smoking men undergoing fertility treatment should stop smoking to increase their chances of having offspring.

  10. Effect of organic and inorganic forms of selenium in diets on turkey semen quality.

    Science.gov (United States)

    Slowińska, M; Jankowski, J; Dietrich, G J; Karol, H; Liszewska, E; Glogowski, J; Kozłowski, K; Sartowska, K; Ciereszko, A

    2011-01-01

    The effects of Se supplementation and its organic or inorganic form on semen quantitative parameters (ejaculate volume, sperm concentration, and total number of sperm) and biochemical parameters of seminal plasma (protein concentration, acid phosphatase activity, superoxide dismutase activity, and total antioxidant capacity) were investigated over a 25-wk reproductive season. Additionally, DNA fragmentation and motility characteristics of turkey spermatozoa were measured. The parameters of turkey semen in relation to yellow semen syndrome were also determined. Twenty-four males (Big 6) were divided into 3 experimental groups differing in form of Se supplementation (no Se supplementation, 0.3 mg/kg of inorganic Se from sodium selenite and 0.3 mg/kg of organic Se from Sel-Plex, Alltech Inc., Nicholasville, KY). Dietary Se supplementation enhanced the sperm concentration and total number of sperm and did not influence the antioxidative properties of turkey seminal plasma and most biochemical parameters. Only seminal plasma acid phosphatase activity was increased in turkeys fed inorganic Se. The main sperm DNA fragmentation parameters were not affected by dietary Se. The highest percentage of motile spermatozoa (85%) was recorded for the semen of turkeys fed organic Se. Values of the biochemical parameters (acid phosphatase, superoxide dismutase, total antioxidant capacity) of seminal plasma increased during the reproductive season. Yellow semen was characterized by increased biochemical parameters and decreased spermatozoa motility characteristics. However, the percentage of motile spermatozoa did not differ between white and yellow semen. Organic Se seemed to be the preferred form of diet supplementation in comparison with inorganic Se. Biochemical parameters of semen and spermatozoa motility parameters appear to be useful for evaluating the effect of age on semen quality. Monitoring the DNA fragmentation of spermatozoa at the end of the reproductive season could be

  11. Eficacia de cinco desinfectantes para la reducción bacteriana doméstica Efficacy of five disinfectants to reduce bacterial load in the household

    Directory of Open Access Journals (Sweden)

    Julián Stambullian

    2011-06-01

    Full Text Available El uso adecuado de hipoclorito de sodio, sales de amonio cuaternario y triclosán ha demostrado ser eficaz para eliminar gérmenes dentro del hogar. Nuestro objetivo fue evaluar la eficacia inmediata, a la semana y al mes del uso controlado de cinco productos con estos componentes, comparados con otros productos de uso habitual. Se incluyeron 32 hogares de clase media de la Ciudad de Buenos Aires y la periferia en un estudio con intervención, abierto, aleatorizado, y de grupos paralelos, durante 6 meses. La mitad de los hogares fue seleccionada para usar hipoclorito de sodio, sales de amonio cuaternario y triclosán en la cocina y el baño durante un mes. El grupo control mantuvo prácticas habituales de limpieza doméstica. Se tomaron muestras para recuento y tipificación bacteriana de los sitios estudiados: muestras basales (sin discriminación de grupo en cocina, que presentaron recuento bacteriano promedio de 66.0 UFC/cm²; baño: 40.1 UFC/cm². Las muestras inmediatas a la limpieza (sin discriminación de grupo: en cocina: 0.8 UFC/cm²; baño: The proper use of products containing sodium hypochlorite, ammonium salts and triclosan has proved to be effective in the elimination of infectious agents in the household environment. Our objective was to evaluate the immediate, one-week and one-month efficacy of controlled use of five products containing these components, compared to other commonly used products. Within a six month period, thirty two middle-class homes from Buenos Aires City and suburbs were included in this open-label, randomized, parallel-group intervention study. Sixteen homes were randomized to use products containing sodium hypochlorite, ammonia and triclosan in the kitchen and bathroom during one month. The remaining maintained usual practices for domestic cleaning. Bacterial counts and identification were performed from samples taken from each study site. Baseline samples (no group discrimination contained a mean bacterial

  12. Cryobiology and a new look at the preservation of stallion semen

    DEFF Research Database (Denmark)

    Grout, Brian William Wilson; Lehn-Jensen, Henrik; Petersen, Morten Rønn

    2009-01-01

      During freeze-preservation of high-viability ejaculates of horse semen the duration of the equilibration time for nucleated straws (achieved at -7 0C following induced nucleation and during controlled, slow cooling to -60 0C) has little impact on viability, measured using propidium iodide...... is discussed and new approaches for successful cryopreservation of semen of low viability are considered.   Keywords Equine spermatozoa; Cryopreservation; Nucleation; Osmotic stress; Repeated freezing...

  13. Effects of semen storage and separation techniques on sperm DNA fragmentation

    OpenAIRE

    Robert E. Jackson; Bormann, Charles L.; Hassun, Pericles A.; Rocha, Andre M.; Motta, Eduardo L. A.; Serafini, Paulo C.; Smith, Gary D.

    2010-01-01

    Objective: To determine the effect of semen storage and separation techniques on sperm DNA fragmentation.Design: Controlled clinical study.Setting: An assisted reproductive technology laboratory.Patient(s): Thirty normoozospermic semen samples obtained from patients undergoing infertility evaluation.Intervention(s): One aliquot from each sample was immediately prepared (control) for the sperm chromatin dispersion assay (SCD). Aliquots used to assess storage techniques were treated in the foll...

  14. Evaluation of semen quality in 1808 university students, from Wuhan, Central China

    Institute of Scientific and Technical Information of China (English)

    Meng Rao; TianQing Meng; SiHeng Hu; HuangTao Guan; QinYu Wei; Wei Xia; ChangHong Zhu; ChengLiang Xiong

    2015-01-01

    The aim of this study was to evaluate the semen quality of university students in Wuhan, the largest city in the world in terms of the number of university students. All student sperm donors recorded in the Hubei Province Human Sperm Bank from 1 March 2010 to 31 December 2013 were screened. At last, a total of 3616 semen samples from 1808 university student sperm donors were eligible and retrospectively analyzed. Each donor’s semen parameters were averaged over two samples and compared with the World Health Organization criteria, and a generalized linear regression model was used to examine several determinants of semen quality. We found that the mean and median values were 3.0 ml and 2.8 ml for semen volume, 50.2 × 106 ml−1 and 50.0 × 106 ml−1 for sperm concentration, 148.1 × 106 and 142.1 × 106 for total sperm count, and 58.6% and 60.0% for total sperm motility. About 85.0% of donors had parameters that were all normal. Season and duration of abstinence were critical factors affecting semen quality. We also found a decrease in sperm concentration during the 4 years observation; however, this may not be a strong evidence to confirm the declining trend of semen quality. In conclusion, semen quality of university students in Wuhan was not optimal and should be paid high attention, long‑term observation and further study should be carried out to confirm the present situation.

  15. Wet heat exposure: a potentially reversible cause of low semen quality in infertile men

    OpenAIRE

    2007-01-01

    OBJECTIVE: To evaluate the recovery of semen quality in a cohort of infertile men after known hyperthermic exposure to hot tubs, hot baths or whirlpool baths. MATERIALS AND METHODS: A consecutive cohort of infertile men had a history remarkable for wet heat exposure in the forms of hot tubs, Jacuzzi or hot baths. Clinical characteristics and exposure parameters were assessed before exposure was discontinued, and semen parameters analyzed before and after discontinuation of hyperthermic exposu...

  16. Characteristics of seminal plasma and cryopreservation of anoa (Bubalus sp. semen obtained by electroejaculation

    Directory of Open Access Journals (Sweden)

    Yudi

    2011-03-01

    Full Text Available The population of anoa, which is an endemic fauna to Indonesia, was getting decrease caused by the illegal hunting and deforestation. Anoa is included in endangered species by IUCN, and Appendix I by CITES. The experiment aimed to characterize the seminal plasma contents and to cryopreserve the anoa semen for artificial insemination application in captivity. The experiment was carried out in Taman Safari Indonesia (Bogor. Semen was collected from 2 anesthetized males (4-10 years by electroejaculation. Seminal plasma gained by centrifugation of ejaculate (3000 rpm, 20 minutes, and then was evaluated the biochemical contents. Other ejaculates were evaluated macroscopically and microscopically, and then extended in Tris and Na-citrate media to a total concentration of 100 billion cells mL-1. Extended semen was stored at 4oC, and evaluated the motility and viability every 12 h. Frozen semen was made in Tris medium added with 5% of glycerol. The seminal plasma of anoa contained total lipid, Na, Ca and Mg higher than the buffalo, but its total protein, K and Cl were lower. Electrophoresis of seminal plasma using by SDS-PAGE method showed 10 bands of proteins (17-148 kDa. The motility and viability of chilled-extended semen in Tris and Na-citrate media were not significantly different (P > 0.05 during 72 h of evaluation. Extended semen in both of media may applicable for AI program for 24-48 h. Post thawing motility of frozen semen was still low, 26.00 ± 9.62%. Therefore, it is necessary to improve each stages of semen processing, so the motility will increased and resulted high pregnancy in AI program.

  17. Evaluation of sperm DNA damage in bulls by TUNEL assay as a parameter of semen quality

    OpenAIRE

    TAKEDA, Kumiko; UCHIYAMA, Kyoko; KINUKAWA, Masashi; Tagami, Takahiro; Kaneda, Masahiro; Watanabe, Shinya

    2015-01-01

    Sperm DNA damage affects the conception rate resulting from human assisted reproduction technology. The objective of this study was to adapt the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay to provide a quality parameter for bull semen based on the detection of sperm DNA damage. Fresh semen was collected from two Japanese Black bulls (A, B) several times over the course of a year, and the percentage of TUNEL-positive spermatozoa (sperm TUNEL index) was d...

  18. The Association between Dietary Patterns and Semen Quality in a General Asian Population of 7282 Males

    OpenAIRE

    Chin-Yu Liu; Yu-Ching Chou; Jane C-J Chao; Chien-Yeh Hsu; Tai-Lung Cha; Chih-Wei Tsao

    2015-01-01

    Objective To explore the associations between different dietary patterns and semen quality in a general Asian male population. Methods Cross-sectional study. Healthy Taiwanese men aged 18 years or older who participated in a standard medical screening program from 2008-2013 run by a private firm were included in this study. Semen parameters including sperm concentration (SC), total sperm motility (TSM), progressive motility (PRM) and normal sperm morphology (NSM) were recorded. A dietary ques...

  19. The Type of Container and Filling Method Have Consequences on Semen Quality in Swine AI Doses

    Directory of Open Access Journals (Sweden)

    Iulian Ibanescu

    2016-05-01

    Full Text Available The automatic filling of semen doses for artificial insemination in swine shows economic advantages over the old-style, manual filling. However, no data could be found regarding the impact, if any, of this packing method on semen quality. This study aimed to compare two types of containers for boar semen, namely the automatically-filled tube and the manually-filled bottle, in terms of preserving the quality of boar semen. Five ejaculates from five different boars were diluted with the same extender and then divided in two aliquots. First aliquot was loaded in tubes filled by an automatic machine while the second was loaded manually in special plastic bottles. The semen was stored in liquid state at 17°C, regardless of the type of container and examined daily, for five days of storage by means of a computer-assisted sperm analyzer. Both types of containers maintained the semen within acceptable values, but after five days of storage significant differences (p<0.05 between the container types were observed in terms of all selected kinetic parameters. The tube showed better values for sperm motility and velocity, while the bottle showed superior values for straightness and linearity of sperm movement. The automatically-filled tubes offered better sperm motility in every day of the study. Given the fact that sperm motility is still the main criterion in assessing semen quality in semen production centers, the main conclusion of this study is that the automatic loading in tubes is superior and recommended over the old-style manual loading in bottles.

  20. Efecto del tipo de resina y del tipo de matriz en la rugosidad superficial de prótesis dentales fijas provisionales confeccionadas con la técnica individualizada

    OpenAIRE

    Ayuso Montero, Raúl

    2007-01-01

    La rugosidad superficial es una de las características más importantes a tener en cuenta en las restauraciones provisionales de prótesis fija, ya que influye en la acumulación de placa bacteriana y en la estética. Se han estudiado diferentes protocolos de pulido para conseguir rugosidades menores, pero se desconoce el efecto del uso de diferentes matrices sobre la rugosidad de las resinas. Los objetivos del estudio fueron evaluar y comparar la rugosidad de dos resinas de metacrilato y una de ...

  1. Semen parameters can be predicted from environmental factors and lifestyle using artificial intelligence methods.

    Science.gov (United States)

    Girela, Jose L; Gil, David; Johnsson, Magnus; Gomez-Torres, María José; De Juan, Joaquín

    2013-04-01

    Fertility rates have dramatically decreased in the last two decades, especially in men. It has been described that environmental factors as well as life habits may affect semen quality. In this paper we use artificial intelligence techniques in order to predict semen characteristics resulting from environmental factors, life habits, and health status, with these techniques constituting a possible decision support system that can help in the study of male fertility potential. A total of 123 young, healthy volunteers provided a semen sample that was analyzed according to the World Health Organization 2010 criteria. They also were asked to complete a validated questionnaire about life habits and health status. Sperm concentration and percentage of motile sperm were related to sociodemographic data, environmental factors, health status, and life habits in order to determine the predictive accuracy of a multilayer perceptron network, a type of artificial neural network. In conclusion, we have developed an artificial neural network that can predict the results of the semen analysis based on the data collected by the questionnaire. The semen parameter that is best predicted using this methodology is the sperm concentration. Although the accuracy for motility is slightly lower than that for concentration, it is possible to predict it with a significant degree of accuracy. This methodology can be a useful tool in early diagnosis of patients with seminal disorders or in the selection of candidates to become semen donors.

  2. Endocrinological, biophysical, and biochemical parameters of semen collected via masturbation versus sexual intercourse.

    Science.gov (United States)

    Sofikitis, N V; Miyagawa, I

    1993-01-01

    In clinical programs of assisted reproduction involving infertile males, it is essential to obtain semen of maximum quality. To evaluate ways of achieving this objective, and to assess the fertilizing capacity of the sperm, six semen samples were collected from each of 38 infertile men via masturbation. Six more samples were then collected from each man at sexual intercourse using a semen collection device (SCD). We confirmed that the volume of seminal plasma, total sperm count, sperm motility, and percentage of morphologically normal spermatozoa were significantly higher in samples collected at intercourse than masturbation, as reported previously. In addition, the markers of the secretory function of the prostate and the outcome of sperm function tests (hypoosmotic swelling test, acrosin assay, and sperm penetration assay) were significantly higher for the samples collected at intercourse. There were no significant differences in markers of the secretory function of the seminal vesicles and epididymis between the samples. The improved spermatozoal parameters in the samples collected at intercourse may reflect a higher prostatic secretory function at that time. There were no significant differences in the serum concentrations of gonadotropins, or in the serum or seminal plasma concentrations of testosterone, before or after masturbation or sexual intercourse. Therefore, the differences in prostatic secretory function and semen parameters may not be attributed to differences in hormonal levels. Semen collection during intercourse using an SCD appears to be the method of choice for selecting semen samples for artificial insemination.

  3. Automation of semen analysis using flow cytometer in comparison with manual methods.

    Science.gov (United States)

    Saleh, Mohamed; Fathy, Amal; El-Akras, Atef I; Eyada, Mostafa M; Younes, Soha; El-Gohary, Ahmed M

    2005-01-01

    In order to standardize techniques and limit the effect of human factors on the results of analyses of biological fluids, automation seems to be mandatory. In an attempt to automate semen analysis, computer assisted sperm analysis (CASA) system has been developed, however its use is still limited and its practical applications have many criticisms. In a trial to automate semen analysis, this study aimed to evaluate the usefulness of flow cytometer in the detection of some seminal parameters in comparison with the traditional manual methods. Isolated spermatogenic cells and isolated sperms from semen and EDTA blood of volunteers were analyzed by flow cytometer in order to define their respective regions. Ejaculates of 28 male patients were subjected to routine semen analyses, leucocytes detection by peroxidase test and monoclonal antibody CD53 using flow cytometer after preparation of the patients' semen samples for flow cytometeric analysis. A highly significant correlation (r=0.96, p= 0.001) of absolute neutrophils (pus cells) detected by peroxidase versus flow cytometer using CD53 monoclonal antibody. A poor correlation (r=0.39, p=0.035) of sperm counts assessed by manual technique and flow cytometer and a spurious sperm counts of 1.08 million/ml detected by flow cytometery in azoospermic patients. Flow cytometer could be used for the assessment of pus cells in semen but seems to be non reliable for the assessment of sperm count if gating depend on sperm size and granularity alone.

  4. Individual cryopreservation with dimethyl sulfoxide and polyvinylpyrrolidone of ejaculates and pooled semen of three avian species.

    Science.gov (United States)

    Herrera, J A; Quintana, J A; López, M A; Betancourt, M; Fierro, R

    2005-01-01

    Artificial insemination (AI) has been used for avian reproduction due to the discovery of cryoprotectants extending its usefulness both in production of domestic fowl and conservation of wild species. The goal of this study was to assess the effect on domestic and wild fowl pooled semen and individual ejaculate cryopreservation with dimethyl sulfoxide (DMSO) and polyvinylpyrrolidone (PVP). Twenty ejaculates and twenty samples of pooled semen of roosters, pheasants and hawks were frozen in media containing DMSO or PVP. DMSO and PVP cryopreservation are equally effective both for ejaculates and pooled semen. Even PVP is a good alternative since no significant difference was found when compared to DMSO. The fertilizing capacity of fresh and cryopreserved pooled semen was analyzed through AI of hens and female pheasants. Similar fertility rates using DMSO, PVP or frozen-thawed samples demonstrated that reproduction is possible through the use of cryopreserved semen. In the case of female pheasants, the same values were obtained with both cryopreserved and fresh semen.

  5. Evaluation of Semen Quality of Holstein Friesian and Jersey Bulls Maintained under Subtropical Environment

    Directory of Open Access Journals (Sweden)

    M. Fiaz*, R. H. Usmani1, M. Abdullah and T. Ahmad2

    2010-04-01

    Full Text Available Semen production data of Holstein-Friesian and Jersey bulls collected over a period of three years was analyzed to examine seasonal effects on quantity and quality of semen. Purebred breeding bulls of Holstein-Friesian and Jersey breeds (n = 18 for each breed, maintained under naturally ventilated open-sided sheds, were used for semen collection. Meteorological information on ambient temperature, relative humidity and rainfall was used to divide the calendar year into two stressful summer seasons viz; dry summer (April - June and wet summer (July–September and a stress free season (October - March. The number of ejaculates per bull recorded during three seasons were not significantly different (P>0.05 in both breeds. Friesian bulls produced lower ejaculatory volume during dry summer season, whereas Jersey bulls produced higher (P0.05 but in Jersey bulls it was lower during wet summer than other seasons. Wet summer resulted in reduced number of semen doses frozen per bull in both breeds. The results of this study indicated that wet summer season deteriorated semen quality in terms of mass motility, individual motility and number of doses in both breeds, except individual motility in semen of Friesian bulls.

  6. Effect of prolonged freezing of semen on exosome recovery and biologic activity.

    Science.gov (United States)

    Welch, Jennifer L; Madison, Marisa N; Margolick, Joseph B; Galvin, Shannon; Gupta, Phalguni; Martínez-Maza, Otoniel; Dash, Chandravanu; Okeoma, Chioma M

    2017-03-24

    Exosomes are important vehicles of intercellular communication that shape host responses to physiologic, tumorigenic, and pathogenic conditions. The composition and function of exosomes are dynamic and depends on the state and condition of the cellular source. In prior work, we found that semen exosomes (SE) from healthy donors who do not use illicit drugs potently inhibit HIV-1. Following semen donation, specimens are either used immediately or frozen for use at a later time. It has been shown that short-term freezing of semen has no effect on SE-mediated HIV-1 inhibition. However, the effect of illicit drugs and prolonged freezing on SE bioactivity is unknown. Here, we show preservation of SE physical properties, (morphology, concentration, intensity/size) irrespective of illicit drug use or duration of semen freezing. Interestingly, illicit drugs and prolonged freezing decreased the levels of SE-bound CD63/CD9 and acetylcholinesterase activity respectively. Furthermore, we show differential effects of illicit drug use and prolonged freezing on SE-mediated HIV-1 inhibition. Our results highlight the importance of the source of SE and condition of semen storage on SE content and function. In-depth evaluation of donor drug-use and duration of semen storage on SE cargo and bioactivity will advance our understanding of SE composition and function.

  7. Effect of prolonged freezing of semen on exosome recovery and biologic activity

    Science.gov (United States)

    Welch, Jennifer L.; Madison, Marisa N.; Margolick, Joseph B.; Galvin, Shannon; Gupta, Phalguni; Martínez-Maza, Otoniel; Dash, Chandravanu; Okeoma, Chioma M.

    2017-01-01

    Exosomes are important vehicles of intercellular communication that shape host responses to physiologic, tumorigenic, and pathogenic conditions. The composition and function of exosomes are dynamic and depends on the state and condition of the cellular source. In prior work, we found that semen exosomes (SE) from healthy donors who do not use illicit drugs potently inhibit HIV-1. Following semen donation, specimens are either used immediately or frozen for use at a later time. It has been shown that short-term freezing of semen has no effect on SE-mediated HIV-1 inhibition. However, the effect of illicit drugs and prolonged freezing on SE bioactivity is unknown. Here, we show preservation of SE physical properties, (morphology, concentration, intensity/size) irrespective of illicit drug use or duration of semen freezing. Interestingly, illicit drugs and prolonged freezing decreased the levels of SE-bound CD63/CD9 and acetylcholinesterase activity respectively. Furthermore, we show differential effects of illicit drug use and prolonged freezing on SE-mediated HIV-1 inhibition. Our results highlight the importance of the source of SE and condition of semen storage on SE content and function. In-depth evaluation of donor drug-use and duration of semen storage on SE cargo and bioactivity will advance our understanding of SE composition and function. PMID:28338013

  8. Escitalopram treatment for premature ejaculation has a negative effect on semen parameters.

    Science.gov (United States)

    Koyuncu, H; Serefoglu, E C; Yencilek, E; Atalay, H; Akbas, N B; Sarıca, K

    2011-01-01

    The aim of this study was to determine the impact of long-term escitalopram treatment on semen parameters of patients with lifelong premature ejaculation (PE). Between November 2008 and January 2010, patients admitted to urology outpatient clinic with a self-reported complaint of PE were evaluated. Medical and sexual history of patients were recorded and patients with lifelong PE (a total of 25 patients) who met the International Society of Sexual Medicine definition were asked to record their intravaginal ejaculatory latency time (IELT) for 1 month, complete Premature Ejaculation Diagnostic Tool (PEDT) questionnaire and give semen samples. Afterwards, patients received 10 mg escitalopram daily for 12 weeks and were invited for control visits at first and third month of treatment. During control visits, PEDT was administered again whereas IELTs were recorded and semen samples were re-examined. PEDT scores, arithmetic means of IELTs and results of semen analyses, which were recorded at baseline, first and third month were compared. At the third month of treatment, a significant increase in mean IELTs and a significant decrease in PEDT scores were detected. However there was a significant decrease in sperm concentration, motility and morphology when compared with the baseline semen measures. Daily escitalopram treatment effects the semen parameters of patients with lifelong PE. Further investigations with larger series are needed to see whether other serotonin reuptake inhibitors have similar side effects and to expose the exact mechanism underlying it. Different treatment modalities should be suggested to patients who desire fertility.

  9. Presence of aerobic micro-organisms and their influence on basic semen parameters in infertile men.

    Science.gov (United States)

    Filipiak, E; Marchlewska, K; Oszukowska, E; Walczak-Jedrzejowska, R; Swierczynska-Cieplucha, A; Kula, K; Slowikowska-Hilczer, J

    2015-09-01

    Urogenital tract infections in males are one of the significant etiological factors in infertility. In this prospective study, 72 patients with abnormal semen parameters or any other symptoms of urogenital tract infection were examined. Semen analysis according to the WHO 2010 manual was performed together with microbial assessment: aerobic bacteria culture, Chlamydia antigen test, Candida culture, Ureaplasma and Mycoplasma-specific culture. In total, 69.4% of semen samples were positive for at least one micro-organism. Ureaplasma sp. was the most common micro-organism found in 33% of semen samples of infertile patients with suspected male genital tract infection. The 2nd most common micro-organisms were Enterococcus faecalis (12.5%) and Escherichia coli (12.5%), followed by Staphylococcus aureus (7%), Chlamydia trachomatis (7%) and Candida sp. (5.6%). Generally, bacteria were sensitive to at least one of the antibiotics tested. No statistically significant relationship was observed between the presence of aerobic micro-organisms in semen and basic semen parameters: volume, pH, concentration, total count, motility, vitality and morphology.

  10. Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction

    Institute of Scientific and Technical Information of China (English)

    Wei-Ping Qian; Li-Ka Shing; Yue-Qiu Tan; Ying Chen; Ying Peng; Zhi Li; Guang-Xiu Lu; Marie C. Lin; Hsiang-Fu Kung; Ming-Ling He

    2005-01-01

    AIM: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen.METHODS: Hepatitis B viral DNA was isolated from HBV carriers' semen and sera using phenol extraction method and QTAamp DNA blood mini kit (Qiagen, Germany). HBV DNA was detected by conventional PCR and quantified by TaqMan technology-based real-time PCR (quantitative polymerase chain reaction (qPCR)). The detection threshold was 200 copies of HBV DNA for conventional PCR and 10 copies of HBV DNA for real time PCR per reaction.RESULTS: Both methods of phenol extraction and QIAamp DNA blood mini kit were suitable for isolating HBV DNA from semen. The value of the detection thresholds was 500 copies of HBV DNA per mL in the semen. The viral loads were 7.5×107 and 1.67×107 copies of HBV DNA per mL in two HBV infected patients' sera, while 2.L4×105 and 3.02×105 copies of HBV DNA per mL in the semen.CONCLUSION: Real-time PCR is a more sensitive and accurate method to detect and quantify HBV DNA in the semen.

  11. Collection, analysis and cryopreservation of semen from Malayan gaur (Bos gaurus hubbacki: A preliminary study

    Directory of Open Access Journals (Sweden)

    M.S. Khairiah

    2012-10-01

    Full Text Available The Malayan gaur (Bos gaurus hubbacki or Seladang is classified as vulnerable by the International Union for Conservation of Nature and Natural Resources (IUCN. The Malayan gaur is mainly distributed in the tropical woodlands of Peninsular Malaysia and Southern Thailand. The aim of this study was to collect, analyze and cryopreserve the semen of wild Malayan gaur. Transrectal massage (TM and electroejaculation (EEJ technique was applied in semen collection of the Malayan gaur. The semen was then cryopreserved in liquid nitrogen using slow freezing technique. Makler counting chamber was used to evaluate sperm concentration and motility, while the sperm viability and morphology of fresh and post-thaw sperm was determined using eosin-nigrosin staining protocol. As a result, we have successfully collected the Malayan gaur semen using EEJ technique. Sperm motility, viability and morphological changes of the post-thaw semen of Malayan gaur were found undesirable due to the complication of the cryopreservation process. On the basis of current study it can be concluded that Malayan gaur bulls semen can be obtain by EEJ with no evidence of rectal trauma. Optimization of the process of cryopreservation for Malayan gaur sperm is needed to maintain the cryoviability of the good sperm quality. The data generated in this study would be useful in conservation of genetic diversity program for Malayan gaur.

  12. Importancia clínica de la translocación bacteriana Clinical importance of bacterial translocation

    Directory of Open Access Journals (Sweden)

    A. García de Lorenzo y Mateos

    2007-05-01

    Full Text Available El tracto gastrointestinal está colonizado por una inmensa cantidad de microorganismos, a los que denominamos flora intestinal. Aunque el intestino proporciona una barrera funcional entre estos organismos y el huésped, la traslocación bacteriana no es un evento infrecuente entre las personas sanas. Sin embrago, en los pacientes críticamente enfermos, que son portadores de diferentes entidades nosológicas, la translocación bacteriana puede condicionar infecciones e incrementos en la morbi-mortalidad. Varios son los mecanismos propuestos para explicar la etiología, génesis y vías de esta entidad y, frecuentemente, los resultados de las investigaciones realizadas tanto in vitro como en animales de experimentación son controvertidos y de difícil aplicación a los humanos. Muchas enfermedades se han relacionado o están implicadas en el fenómeno de translocación pero desgraciadamente el sumatorio de estudios metodológicamente insuficientes, poblaciones reducidas y resultados conflictivos dejan, hoy en día, muchas preguntas abiertas y otras sin respuesta lógica. Esta problemática también se refleja a la hora de estudiar y valorar las diferentes estrategias terapéuticas utilizadas así como los métodos empleados para detectar la traslocación.The gastrointestinal tract is colonized by a huge number of microorganisms that we call intestinal flora. Although the bowel provides a functional barrier between these organisms and the host, bacterial translocation is not an infrequent event in healthy people. However, in critically ill patients, carriers of different morbid entities, bacterial translocation may favor infections and increased morbimortality. There are several proposed mechanisms explaining the etiology, genesis, and ways for this entity, and frequently the results from both in vitro and animal experimental investigations are controversial and difficult to apply to humans. Many diseases have been linked or are implicated in

  13. Sensibilidade a cobre, estreptomicina e oxitetraciclina em Xanthomonas spp. associadas à mancha-bacteriana do tomate para processamento industrial

    Directory of Open Access Journals (Sweden)

    Quezado-Duval Alice Maria

    2003-01-01

    Full Text Available Apesar de amplamente empregados em lavouras de tomate para processamento industrial no Brasil, fungicidas cúpricos e antibióticos registrados para uso agrícola nem sempre resultam em controle eficiente das bacterioses que afetam a cultura. O aparecimento de estirpes resistentes é uma das causas dessa baixa eficiência. Avaliou-se, in vitro, a sensibilidade a cobre, estreptomicina e oxitetraciclina de 389 isolados de Xanthomonas spp. associadas à mancha-bacteriana do tomateiro, sendo 92 de X. axonopodis pv. vesicatoria (60 do grupo "A"/raça T1 e 32 do "C"/raça T3, 93 de X. vesicatoria (grupo "B"/raça T2 e 204 de X. gardneri (grupo "D"/raça T2. Os isolados foram obtidos de plantas doentes em campos comerciais de tomate para processamento industrial nos estados de Goiás, Minas Gerais, Pernambuco e Bahia, nos anos de 1995 a 1998 e em 2000. Alíquotas de 5 ml de suspensões bacterianas foram depositadas em meio Nutriente-Ágar suplementado com sulfato de cobre, nas concentrações de 50 e 200 µg/ml; sulfato de estreptomicina, a 25 e 200 µg/ml e cloridrato de oxitetraciclina, a 25 µg/ml. Nenhum isolado foi resistente a oxitetraciclina, como também nenhum foi resistente ao cobre na concentração de 200 µg/ml do sulfato de cobre. No entanto, houve diferença entre isolados quanto à sensibilidade ao sulfato de cobre na concentração de 50 µg/ml e ao sulfato de estreptomicina nas duas concentrações empregadas. As freqüências de isolados de X. gardneri, X. axonopodis pv. vesicatoria (grupos "A" e "C" e X. vesicatoria resistentes à estreptomicina (25 µg/ml do produto usado foram, respectivamente, 98%, 38% e 2%, ao passo que, ao cobre, foram, respectivamente, 48%, 4% e 74%. Todos os isolados do grupo "C" foram sensíveis à estreptomicina e 97% sensíveis ao cobre.

  14. Molecular characterization of ruminal bacterial diversity in vitro = Caracterização molecular da diversidade bacteriana ruminal in vitro

    Directory of Open Access Journals (Sweden)

    Humberto Maciel França Madeira

    2008-04-01

    Full Text Available PCR analysis is a sensitive and specific tool to detect and monitormicroorganisms in complex environmental samples. The amplification of 16S ribosomal DNA sequences followed by gel electrophoresis under denaturing gradient (DGGE has been a powerful technique to genetically evaluate microbial ecosystems. Changes in rumenmicrobial populations were investigated in vitro using a basal diet with different lipid sources. PCRs were performed with two different sets of primers in order to amplify 16S rRNA sequences, and the amplified fragments were submitted to DGGE analysis. The findings presented in this study show that distinct microbial communities were present in each treatment. The presence of soybean oil seems to maximize growth of bacterial population, whereas fish oil appears to reduce growth. We demonstrated the successful application of molecular ecological techniques to analyze the structure and composition of bacterial communities in rumen ecosystems.A análise por PCR fornece um meio sensível e específico para detectar e monitorar microrganismos em amostras ambientais complexas. Desde sua aplicação inicial, o DNA ribossomal 16S (rRNA em eletroforese com gel com gradiente desnaturante (DGGE, tem sido uma técnica atrativa para a ecologia molecular microbiana. Foram investigadas mudanças na população microbiana no rúmen, a partir de alterações da dieta com tratamentos in vitro de diferentes fontes de lipídeos. ODGGE foi testado com dois pares de primers para o rRNA 16S. O uso do fragmento de 200 pb gerou um perfil de bandas mais discriminatório, mostrando que diferentes comunidades microbianas estavam presentes entre os tratamentos in vitro analisados. A presença de óleo de sojapotencializou o crescimento da população bacteriana, enquanto que óleo de peixe parece ter reduzido esse crescimento. Foi possível demonstrar o sucesso da aplicação de técnicas moleculares para analisar a estrutura e a composição de comunidades

  15. PERANAN BETA-KAROTEN DALAM MEMPERTAHANKAN DAYA HIDUP SPERMATOZOA SEMEN CAIR DOMBA GARUT ROLE OF BETA-CAROTENE INI MAINTAINING SPERM LIVABILITY OF CHILLED-SEMEN OF GARUT RAM

    Directory of Open Access Journals (Sweden)

    Muhammad Rizal

    2006-12-01

    Full Text Available Tujuan penelitian ini adalah untuk mengevaluasi kualitas semen cair domba garut yang diencerkan dengan larutan pengencer tris dengan penambahan berbagai konsentrasi beta-karoten. Semen dikoleksi dengan vagina buatan satu kali dalam satu minggu dari empat pejantan domba garut dewasa kelamin. Segera setelah dievaluasi, semen segar dibagi ke dalam tiga buah tabung reaksi dan masing-masing diencerkan dengan pengencer tris (kontrol, pengencer tris + 0,0125% beta-karoten

  16. Reviewing reports of semen volume and male aging of last 33 years:From 1980 through 2013

    Institute of Scientific and Technical Information of China (English)

    Pallav; Sengupta

    2015-01-01

    Since several decades numerous experimental and epidemiological experiments tend to establish that in humans the semen volume declines with progression of age. This literature review is intended to report the association between male age and semen volume. Review of English language-published research over the last 33 years, from January 1, 1980, up to December 31, 2013, has been conveniently constructed using MEDLINE database. Studies with inadequate numbers of subjects and case reports were excluded. Among the methodologically stronger studies, declines in semen volume of 3% - 22% were likely when comparing 30-year-old men to 50-year-old men. The report suggests that increased male age is associated with a decline in semen volume, i.e. there has been a genuine diminution in semen volume over the past 33 years. As male fertility is to some extent correlated with semen volume the results may reflect an overall reduction in male fertility.

  17. Genomic selection strategies in dairy cattle breeding programmes: Sexed semen cannot replace multiple ovulation and embryo transfer as superior reproductive technology

    DEFF Research Database (Denmark)

    Pedersen, Louise Dybdahl; Kargo, Morten; Berg, Peer

    2012-01-01

    of using sexed semen on the genetic gain was very small compared with the effect of MOET and highly dependent on whether cow dams or bull dams were inseminated with sexed semen and on what type of semen that was used for the bull dams. The rate of inbreeding was seldom affected by the use of sexed semen....... However, when all young bull candidates were born following MOET, the results showed that the use of Y-semen in the breeding nucleus tended to decrease the rate of inbreeding as it enabled GS to increase within-family selection. This implies that the benefit from using sexed semen in a modern dairy cattle...

  18. Degradación bacteriana de cianuro y compuestos nitrogenados tóxicos

    OpenAIRE

    2016-01-01

    La ruta de asimilación de cianuro en P. pseudoalcaligenes CECT5344 transcurre a través de un nitrilo formado por la reacción química del cianuro con el oxalacetato, siendo este último acumulado como consecuencia de la acción conjunta de una malato:quinona oxidoreductasa (MQO) y la oxidasa terminal resistente a cianuro (CioAB) (Luque-Almagro et al., 2011b). Los nitrilos pueden ser convertidos en amonio por la acción de una nitrilasa o un sistema nitrilo hidratasa/amidasa. ...

  19. Etiología de la diarrea aguda bacteriana en pacientes pediátricos ambulatorios

    OpenAIRE

    Giugno, Silvina; Oderiz, Sebastián

    2008-01-01

    La diarrea aguda es una causa importante de morbilidad y mortalidad en países en desarrollo y en pocos casos requiere tratamiento antimicrobiano. El correcto uso de éstos depende del conocimiento previo de la epidemiología local. Para conocer estos parámetros estudiamos en forma retrospectiva los resultados de las bacterias enteropatógenas aisladas en la ciudad de La Plata. Se estudiaron 7.075 muestras de materia fecal de niños ambulatorios hasta 15 a...

  20. Semen apoptotic M540 body levels correlate with testis abnormalities: a study in a cohort of infertile subjects.

    OpenAIRE

    Lotti F; Tamburrino L; Marchiani S; Muratori M; Corona G; Fino MG; Degl'innocenti S; Forti G; Maggi M; Baldi E.

    2012-01-01

    STUDY QUESTION: What are the associations between semen apoptotic M540 bodies and other parameters of semen quality and sonographic alterations of the male genital tract in a cohort of infertile subjects? SUMMARY ANSWER: In infertile subjects, semen M450 bodies are highly correlated with ultrasound and clinical signs of testis abnormalities but not with alterations of other parts of the male genital tract, suggesting a testicular origin of M540 bodies. WHAT IS KNOWN ALREADY:...

  1. Contextualización de las zoonosis bacterianas y virales transmitidas por roedores

    Directory of Open Access Journals (Sweden)

    Juan David Rodas G

    2010-04-01

    Full Text Available Hace un poco más de 3 años, registramos dentro del postgrado de biología de la Universidad de Antioquia, la línea de investigación en zoonosis emergentes y reemergentes; motivados por los, en aquel entonces, recientes eventos, que aparentemente involucraban la transmisión de Hantavirus desde roedores (1,2, e inspirado por la experiencia recién adquirida (finales del 2004, en investigación de Arenavirus y otros virus asociados con fiebres hemorrágica en laboratorios de Estados Unidos (Universidad de Wisconsin e Instituto de Virología Humana en Baltimore, Maryland (3, nos vimos estimulados a formular y proponer, en convenio con el Instituto Colombiano de Medicina Tropical (ICMT-CES, uno de los primeros estudios de Leptospirosis en roedores urbanos en Medellín (4, y más tarde, también con el ICMT y el laboratorio de Inmuno-virología, el estudio de Hantavirus, Arenavirus y Leptospira en roedores urbanos y silvestres y en humanos de la región de Urabá en Antioquia.

  2. BIODEGRADACIÓN BACTERIANA DE PLAGUICIDAS PERMETRINA Y CIPERMETRINA EN CULTIVO LOTE

    Directory of Open Access Journals (Sweden)

    José C. Mendoza

    2011-01-01

    Full Text Available Fue estudiada la biodegradación de permetrina y cipermetrina a concentraciones de 50 y 100 mg/L, con cepas de Pseudomonas putida, Pseudomonas mendocina, Chromobacterium violaceum y Burkholderia cepacia, en reactores por lotes. Las cepas de Pseudomonas putida y Pseudomonas mendocina fueron las que presentaron una mayor capacidad de biodegradación de los plaguicidas, a partir de los 5 días esta es del 65% para ambos plaguicidas y después de los 15 días se mantiene prácticamente constante, siendo de hasta el 95% para permetrina a 50 y 100 mg/L y para cipermetrina del 90% a 50 mg/L y 89% a 100 mg/L medido mediante espectrofotometría UV/Vis. La cinética de crecimiento y los espectros de infrarrojo muestran que hay una mayor capacidad de mineralización para el plaguicida permetrina que para la cipermetrina. Estos resultados indican que estas bacterias pueden ser usadas en procesos de biorremediación de estos plaguicidas.

  3. Diagnosis and effects of urine contamination in cooled-extended stallion semen.

    Science.gov (United States)

    Ellerbrock, R; Canisso, I; Feijo, L; Lima, F; Shipley, C; Kline, K

    2016-04-15

    Urospermia is known to affect semen quality in many mammals, including stallions. Determinations of semen pH and creatinine and urea concentrations have been used to diagnose urine contamination in raw stallion semen. Unfortunately, practitioners suspecting urine contamination in cooled-shipped samples have no proven means to confirm the presence of urine. Therefore, the objectives of this study were (1) to assess the effects of urine contamination on sperm motility of extended fresh and cooled-stored stallion semen, (2) to evaluate the usefulness of semen color, odor, pH, and creatinine and urea concentrations for urospermia diagnosis, and (3) to evaluate the accuracy of a commercial blood urea nitrogen test strip in diagnosing urine contamination in extended-cooled stallion semen. Thirty-seven ejaculates were obtained from 11 stallions with no history of urospermia before division into 5 mL aliquots, and contamination with stallion urine. Each resulting sample was assessed for sperm motility, color, odor, pH, creatinine, and urea nitrogen concentration using both a semiquantitative test strip (Azostix), and a quantitative automated analyzer before and after cooling for 24 hour. Sperm motility parameters, pH, and creatinine and urea concentrations were analyzed using mixed models. Urine contamination decreased total and progressive motility in all samples before and after cooling (P contaminated samples ranged from 30% to 71% at 0 hour and 27% to 61% at 24 hours. Control mean urea (29 mg/dL) and creatinine (0.6 mg/dL) concentrations were significantly different (P contaminated samples (158 mg/dL and 11.6 mg/dL, respectively) at 0 hour. Similarly, control mean urea (8 mg/dL) and creatinine (0.9 mg/dL) concentrations were significantly different than all urine-contaminated samples at 24 hours. Odor assessment presented moderate sensitivity (65%) and high specificity (100%), while color assessment presented low sensitivity (47%) and moderate specificity (79

  4. Semen-mediated enhancement of HIV infection is donor-dependent and correlates with the levels of SEVI

    Directory of Open Access Journals (Sweden)

    Hahn Beatrice H

    2010-06-01

    Full Text Available Abstract Background HIV-1 is usually transmitted in the presence of semen. We have shown that semen boosts HIV-1 infection and contains fragments of prostatic acid phosphatase (PAP forming amyloid aggregates termed SEVI (semen-derived enhancer of viral infection that promote virion attachment to target cells. Despite its importance for the global spread of HIV-1, however, the effect of semen on virus infection is controversial. Results Here, we established methods allowing the meaningful analysis of semen by minimizing its cytotoxic effects and partly recapitulating the conditions encountered during sexual HIV-1 transmission. We show that semen rapidly and effectively enhances the infectivity of HIV-1, HIV-2, and SIV. This enhancement occurs independently of the viral genotype and coreceptor tropism as well as the virus producer and target cell type. Semen-mediated enhancement of HIV-1 infection was also observed under acidic pH conditions and in the presence of vaginal fluid. We further show that the potency of semen in boosting HIV-1 infection is donor dependent and correlates with the levels of SEVI. Conclusions Our results show that semen strongly enhances the infectivity of HIV-1 and other primate lentiviruses and that SEVI contributes to this effect. Thus, SEVI may play an important role in the sexual transmission of HIV-1 and addition of SEVI inhibitors to microbicides may improve their efficacy.

  5. Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders

    Directory of Open Access Journals (Sweden)

    Guilherme Pugliesi

    2012-12-01

    Full Text Available Two semen extenders were compared for their ability to maintain viability of horse semen during 24 hours of cold preservation, and for the pregnancy rate after artificial insemination. In the experiment 1, five ejaculates from three stallions were split-diluted in either a skimmed milk-based extender (Kenney extender or a glycine egg yolk-based extender (Foote extender and cooled at 6-8 ºC for 24 hours. Semen samples stored in Kenney extender for 24 hours had higher motility and spermatic vigor compared with those stored in Foote extender. However, samples stored in Foote extender had higher number of reactive sperm by hypoosmotic test and greater viability by epifluorescence test compared with those in Kenney extender. In the experiment 2, 17 and 23 ejaculates from two stallions were split-diluted with Kenney extender and Foote extender. The sperm concentration in each extender was adjusted to 500 million viable sperms per insemination dose. Semen was cooled to 6-8 ºC and stored for 24 hours. Seventy-four cycles of crossbred mares were inseminated with either semen diluted in Kenney extender or semen diluted in Foote extender. The pregnancy rate was higher from semen diluted in Kenney extender than that from semen in Foote extender (0.553 vs. 0.306. The Kenney extender is effective in preserving the motility, vigor and fertility of stallion semen after 24 hours of cold storage, whereas the Foote extender is not acceptable.

  6. Isolation, culture and characterisation of somatic cells derived from semen and milk of endangered sheep and eland antelope.

    Science.gov (United States)

    Nel-Themaat, L; Gómez, M C; Damiani, P; Wirtu, G; Dresser, B L; Bondioli, K R; Lyons, L A; Pope, C E; Godke, R A

    2007-01-01

    Semen and milk are potential sources of somatic cells for genome banks. In the present study, we cultured and characterised cells from: (1) cooled sheep milk; (2) fresh, cooled and frozen-thawed semen from Gulf Coast native (GCN) sheep (Ovis aries); and (3) fresh eland (Taurotragus oryx) semen. Cells attached to the culture surface from fresh (29%), cooled (43%) and slow-frozen (1 degrees C/min; 14%) ram semen, whereas no attachment occurred in the fast-frozen (10 degrees C/min) group. Proliferation occurred in fresh (50%) and cooled (100%) groups, but no cells proliferated after passage 1 (P1). Eland semen yielded cell lines (100%) that were cryopreserved at P1. In samples from GCN and cross-bred milk, cell attachment (83% and 95%, respectively) and proliferation (60% and 37%, respectively) were observed. Immunocytochemical detection of cytokeratin indicated an epithelial origin of semen-derived cells, whereas milk yielded either fibroblasts, epithelial or a mixture of cell types. Deoxyribonucleic acid microsatellite analysis using cattle-derived markers confirmed that eland cells were from the semen donor. Eland epithelial cells were transferred into eland oocytes and 12 (71%), six (35%) and two (12%) embryos cleaved and developed to morulae or blastocyst stages, respectively. In conclusion, we have developed a technique for obtaining somatic cells from semen. We have also demonstrated that semen-derived cells can serve as karyoplast donors for nuclear transfer.

  7. Sialoadenitis bacteriana crónica recurrente de la infancia. Caracterización clínica

    Directory of Open Access Journals (Sweden)

    Carlos Ávila-Sánchez

    2015-03-01

    Full Text Available La sialoadenitis recurrente es el segundo cuadro inflamatorio más frecuente que afecta a la glándula parótida, precedida por la parotiditis viral. Esta afección tiene un cuadro clínico muy similar a la sialoadenitis, motivo por el cual se debe considerar dentro de los  iagnósticos diferenciales. La sialoadenitis recurrente se caracteriza por episodios periódicos de inflamación y aumento de volumen de la glándula parótida acompañados de fiebre y malestar general que alternan con intervalos de remisión en los que la glándula es clínicamente asintomática. En el Instituto Nacional de Pediatría consideramos al concepto Sialoadenitis bacteriana crónica recurrente de la infancia como la definición más específica y completa para referirse a esta enfermedad. Debido a su recurrencia decidimos escribir este artículo para señalar sus características clínicas.

  8. Sobre la resistencia bacteriana hacia antibióticos de acción bactericida y bacteriostática

    Directory of Open Access Journals (Sweden)

    Jhoana P. Romero L.

    2014-06-01

    Full Text Available En este artículo se formula un modelo matemático simple que describe la interacción entre bacterias sensibles y resistentes a múltiples antibióticos de acción bactericida y bacteriostática de forma simultánea, en el supuesto de que la adquisición de resistencia bacteriana se da a través de mutaciones espontáneas y adquiridas por la exposición a diferentes antibióticos. El análisis cualitativo revela la existencia de un equilibrio libre de bacterias, un equilibrio solo con bacterias resistentes y un equilibrio endémico donde coexisten ambas poblaciones de bacterias. Abstract. In this work we formulate a simple mathematical model that describes the population dynamics of bacteria exposed simultaneously to multiple bactericidal and bacteriostatic antibiotics, assuming that resistance is acquired through mutations due to antibiotic exposure. Qualitative analysis reveals the existence of a free-bacteria equilibrium, resistant-bacteria equilibrium and an endemic equilibrium where both bacteria coexist.

  9. Protocolo de actuación forense ante la sospecha de meningitis bacteriana y shock séptico fulminante Protocol for the forensic action with regard to the suspicion of bacterial meningitis and fulminant septicemia

    Directory of Open Access Journals (Sweden)

    A. Fernández-Rodríguez

    2004-07-01

    Full Text Available Una importante tarea de las instituciones médico-legales es la colaboración con las autoridades sanitarias en la prevención de riesgos para la salud pública. Uno de ellos es la infección meningocócica, que puede cursar rápidamente y con evolución fatal, causando muerte súbita. Neisseria meningitidis es la principal causa de meningitis bacteriana y septicemia en niños y adultos jóvenes. Su temprana detección es de extrema urgencia, ya que permite el adecuado tratamiento de los contactos. El rápido desarrollo de estas infecciones imposibilita en ocasiones el diagnóstico antemortem, resultando imprescindible el diagnóstico forense. Aunque los hallazgos postmortem suelen ser los del síndrome de Waterhouse-Friderichsen, estos, a veces, pueden pasar desapercibidos, o estar causados por otras bacterias. Todo ello hace necesario un diagnóstico microbiológico forense. Este artículo presenta un protocolo forense de actuación ante la sospecha de meningitis bacteriana y shock séptico fulminante. La toma de muestras para microbiología debe ser aséptica e incluir sangre, suero, LCR, líquido pleural, tejidos en fresco y orina. Estas muestras pueden remitirse al Instituto Nacional de Toxicología y Ciencias Forenses, donde se realizarán análisis específicos, que incluyen un screening antigénico y un diagnóstico de confirmación mediante PCR a tiempo real que identifica meningococo y su serogrupo. Los resultados de estas técnicas son informados 3 horas tras el inicio del análisis. También se realiza el cultivo bacteriano y PCR adicionales para detectar otras bacterias difíciles de aislar postmortem. La colaboración entre las distintas instituciones médico-legales y entre estas y las autoridades sanitarias es esencial para el adecuado manejo de estas graves infecciones.One of the main duties of medical-legal institutions is to collaborate with authorities to recognise and prevent risks to public health. One such risk is

  10. Evaluation and assessment of semen for IVF/ICSI

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Evaluation and assessment of sement is very important for both diagnosis of male infertility and selection of patients for treatment with IVF or ICSI.In standard IVF,sperm function is essential for normal fertilization:sperm must be able to bind to zona pellucida (ZP),undergo the acrosome reaction and penetrate the ZP and fuse with the oolemma before fertilization takes place.In contrast,most sperm functions are not required for fertilization in ICSI since sperm bypass the ZP and oolemma by injection of a single sperm directly into cytoplasm of oocyte.Therefore,the clinical decision on treatment of patients with either IVF or ICSI is mostly dependent on results of sperm tests.However,conventional semen analyses do not provide accurate information about sperm fertilizing ability since many patients with subtle sperm defects can not be detected.More advanced sperm function tests are required to detect sperm defects that may lead to failure of fertilization in standard IVF.In the last 15years wer performed extensive studies on relationship between sperm functions and fertilization rates by logistic regression analysis in large numbers of IVF patients including 370 patients with zero fertilization rate by IVF.We confirmed sperm morphology assessed strictly was strongly related to fertilisation rate with standard IVF.Thus sperm morphology assessment is very useful for selection of patients for ICSI.We also developed a number of new tests including sperm-ZP binding,sperm-ZP penetration and the ZP-induced AR and evaluated the clinical value of these tests.Sperm-ZP binding and sperm-ZP penetration tests are the most powerful indicators for sperm fertilizing ability in vitro.The ZP-induced AR is highly correlated with sperm-ZP peretration.We discovered a condition we call disordered ZP-induced AR which causes serve infertility in up to 25% men with otherwise idiopathic infertility In conclusion,the combination of semen analysis with advanced sperm function tests provide

  11. Single semen analysis as a predictor of semen quality: clinical and epidemiological implications%用单次精液分析预测精液质量:临床及流行病意义

    Institute of Scientific and Technical Information of China (English)

    Lars Rylander; Boel Wetterstrand; Trine B Haugen; Gunilla Malm; Johan Maim; Cathrine Bjφrsvik; Trine Henrichsen; Thomas Saether; Aleksander Giwercman

    2009-01-01

    It is generally thought that a single ejaculate is a bad predictor of semen quality of a subject, because of signifi-cant intra-individual variation. Therefore, we investigated the degree to which the results of a first semen analysis differ from that of a second analysis among men from a general population in Norway. In addition, we analysed how the two different semen results mirrored the overall semen quality assessment. A total of 199 volunteers par-ticipated in the study and delivered two semen samples with an interval of 6 months. The semen parameters were determined according to the World Health Organization (WHO) 1999 guidelines, which were also used to determine whether semen quality was normal or abnormal. In addition, the DNA fragmentation index (DFI) was determined using the Sperm Chromatin Structure Assay. The two samples from each individual were very similar with regard to standard semen parameters and DFI (r_s: 0.67-0.72), and there were no significant systematic differences between the two samples. The result of the first sample (normal/abnormal) was highly predictive of the overall conclusion based on the two samples (sperm concentration: in 93% of the cases (95% confidence interval [CI]: 89%-96%); sperm motility: in 85% of the cases (95% CI: 79%-89%); overall semen quality: in 85% of the cases (95% CI: 80%-90%). In epidemiological studies, one ejaculate is a sufficient indicator of semen quality in a group of subjects. In a clinical situation, when the question is whether the semen quality is normal or not, the first ejaculate will, in at least 85% of cases, give a correct overall conclusion.

  12. Pregnancy in the domestic cat after vaginal or transcervical insemination with fresh and frozen semen.

    Science.gov (United States)

    Chatdarong, K; Axnér, E; Manee-In, S; Thuwanut, P; Linde-Forsberg, C

    2007-12-01

    The objective was to compare pregnancy rates in domestic cats using fresh semen for intravaginal artificial insemination (IVI), either at the time of hCG treatment for induction of ovulation, or 28 h later, and to compare pregnancy rates following IVI or transcervical intrauterine insemination (IUI) of frozen-thawed semen. Eighteen queens were inseminated during 39 estrus cycles. Fresh semen with 13.5+/-5.4 x 10(6) sperm (range, 6.8-22 x 10(6)) collected by electroejaculation from four male cats was used in Experiment 1, and cryopreserved semen (20 x 10(6) sperm, with 70+/-5% post-thaw motility) from one male cat was used in Experiment 2. Serum concentrations of estradiol-17beta and progesterone were determined in most queens on the day of AI and again 30-40 days later. Treatment with 100 IU of hCG 3 days after the onset of estrus induced ovulation in 95% of treated queens. Pregnancy rates to IVI with fresh semen at the time of hCG administration versus 28 h later were not different (P=0.58); overall 33% (5/15) of the queens became pregnant. For frozen-thawed semen, AI was consistently done 28h after hCG administration; IUI and IVI resulted in pregnancy rates of 41.7% (5/12), whereas no queen (0/12) became pregnant by IVI (P=0.0083). In conclusion, an acceptable pregnancy rate was obtained with frozen-thawed semen in the domestic cat by non-surgical transcervical IUI; this method might also be useful in other small felids.

  13. Boar semen bacterial contamination in Italy and antibiotic efficacy in a modified extender

    Directory of Open Access Journals (Sweden)

    Carla Bresciani

    2014-02-01

    Full Text Available The aims of the study were to identify microbial flora in boar semen under field conditions in northern Italy, to investigate antibiotic resistance and sensitivity of isolated bacteria, and to evaluate elimination of bacteria after storage in two types of extenders added with different antibiotics (amikacin vs gentamicin. A total of 60 boars were collected in 13 pig farms. Bacteriological and mycological investigations were performed immediately on raw semen samples, then at 48 and 120 h of storage on semen diluted randomly in a new short-term modified extender (ME-S or in a commercial one (CRONOSTM. Bacterial contamination was found in 63% of raw semen samples and different bacterial species were isolated: E.coli, Serratia marcescens, Staphylococcus epidermidis and aureus, Proteus spp., Streptococcus spp. and Pseudomonas aeruginosa. E. coli was the most isolated contaminant (53%; Pseudomonas aeruginosa was found only in one semen sample. The analysis of variance of factors affecting contamination levels was significant for the farm of origin (P<0.05 and not significant for the breed. Antibiotic resistance of these bacteria was assessed using different antibiotics. Significant differences (P<0.05 between observed and expected frequencies of bacterial isolates resistant or not to the antibiotics contained in the extenders were found. At 48 h of storage a reduction of aerobic contamination was found after ME-S dilution by 85.3% and after CRONOSTM by 63.8%. This paper proved the presence of pathogenic bacteria in semen. We thus believe it is highly advisable to perform periodic microbiological screening of boar semen in the swine industry to avoid the use of low sperm quality.

  14. Liquid semen storage in elephants (Elephas maximus and Loxodonta africana): species differences and storage optimization.

    Science.gov (United States)

    Kiso, Wendy K; Brown, Janine L; Siewerdt, Frank; Schmitt, Dennis L; Olson, Deborah; Crichton, Elizabeth G; Pukazhenthi, Budhan S

    2011-01-01

    Artificial insemination plays a key role in the genetic management of elephants in zoos. Because freshly extended semen is typically used for artificial insemination in elephants, it has become imperative to optimize conditions for liquid storage and semen transport. The objectives of this study were to examine the interactions between different extenders and storage temperatures on sperm total motility, progressive motility, and acrosomal integrity in Asian (Elephas maximus) and African (Loxodonta africana) elephants. Ejaculates were collected by rectal massage, diluted using a split-sample technique in 5 semen extenders: TL-Hepes (HEP), Modena (MOD), Biladyl (BIL), TEST refrigeration medium (TES), and INRA96 (INR), maintained at 35°C, 22°C, or 4°C. At 0, 4, 6, 12, and 24 hours, aliquots were removed and assessed for sperm total motility, progressive motility, and acrosomal integrity. After 24 hours of storage, African elephant spermatozoa exhibited greater longevity and higher values in sperm quality parameters compared with those of Asian elephants. In both species, semen storage at 35°C resulted in a sharp decline in all sperm quality parameters after 4 hours of storage, whereas storage at 22°C and 4°C facilitated sperm survival. In Asian elephants, MOD and HEP were most detrimental, whereas BIL, TES, and INR maintained motility up to 12 hours when spermatozoa were cooled to 22°Cor4°C. In African elephants, there were no differences among extenders. All media maintained good sperm quality parameters at 22°C or 4°C. However, although MOD, BIL, and INR were most effective at lower temperatures, HEP and TES maintained sperm motility at all storage temperatures. This study demonstrated sperm sensitivity to components of various semen extenders and storage temperatures and offers recommendations for semen extender choices for liquid semen storage for both Asian and African elephants.

  15. Genetic Evaluation of Semen and Growth Traits of Young Simmental Bulls in Performance Test

    Directory of Open Access Journals (Sweden)

    Miroslav Kapš

    2000-03-01

    Full Text Available The objective of this study was to estimate genetic and environmental variances and heritabilities for body measurements, daily gain, semen volume and semen concentration of young Simmental bulls. In addition, genetic trends for those traits were analyzed. Data utilized in this study consisted of records of 955 young Simmetal bulls born from 1974 to 1995 and were provided by the Performance Test Station - Varazdin. The test for growth traits started at the age of 120-d, and finished at 365-d. Generally, body measurements were taken every month, but there were animals with lost records. In order to smooth data and predict values at ages of 205- and 365-d, the spline analysis was applied. The following growth traits were derived: weights, heights at withers, hearth girth, and chest depth, predicted at 205- and 365-d of age, respectively. Further, average daily gain from 205- do 365-d was calculated. The semen collection started at approximately 11 months of age. The records included semen volume and concentration. Variance and covariance components and associated heritabilities were estimated by REML from a set of single-trait animal models. Fixed effects were defined as birth year and season, and animal effect was defined as random effect. The heritability estimates for growth traits ranged from .14 to .38. Heritability estimate for semen concentration was moderate (.26. Thus, the improvement of concentration, or at least not negative genetic trend, can be expected even if only phenotypic selection is practiced. This cannot be said for semen volume, as heritability estimate in the present study was low (.04. In this study no clear genetic trend for semen nor growth traits were detected.

  16. Association between a biomarker of exposure to polycyclic aromatic hydrocarbons and semen quality

    Directory of Open Access Journals (Sweden)

    Joanna Jurewicz

    2013-10-01

    Full Text Available Objectives: Growing evidence supports the reproductive and developmental toxicity of polycyclic aromatic hydrocarbons (PAHs from prenatal and postnatal exposure, but the results of epidemiological studies regarding harmful effects of PAHs exposure on male reproductive system still remain limited and inconclusive. The aim of the present study was to investigate the relationship between 1-hydroxypyrene, a biomarker of polycyclic aromatic hydrocarbons exposure and semen quality. Materials and Methods: The study population consisted of 277 men attending an infertility clinic for diagnostic purposes and having normal semen concentration of 20-300 mln/ml or slight oligozoospermia (semen concentration: 15-20 mln/ml (WHO 1999. All the men were healthy and under 45 years of age. All participants were interviewed and provided a semen sample. The interview included questions concerning demographics, socio-economic status, medical history related to past diseases which may have an impact on semen quality, lifestyle factors and occupational information. Concentrations of 1-hydroxypyrene (1-OHP in the urine samples were analyzed using high performance liquid chromatography (HPLC. Results: A positive association was found between the level of 1-OHP in urine and sperm neck abnormalities as well as the percentage of static sperm cells (p = 0.001, p = 0.018, respectively. Additionally, exposure to PAHs measured by 1-OHP in urine decreased semen volume and the percentage of motile sperm cells (p = 0.014, p = 0.0001, respectively. Conclusions: Presented findings indicate that the environmental level of PAHs exposure adversely affects male semen quality. The future large-scale studies should incorporate different biomarkers to generate a more accurate and full assessment of the effects of PAHs exposure on male fertility.

  17. Effect of varicocelectomy on testis volume and semen parameters in adolescents: a meta-analysis

    Directory of Open Access Journals (Sweden)

    Tie Zhou

    2015-01-01

    Full Text Available Varicocele repair in adolescent remains controversial. Our aim is to identify and combine clinical trials results published thus far to ascertain the efficacy of varicocelectomy in improving testis volume and semen parameters compared with nontreatment control. A literature search was performed using Medline, Embase and Web of Science, which included results obtained from meta-analysis, randomized and nonrandomized controlled studies. The study population was adolescents with clinically palpable varicocele with or without the testicular asymmetry or abnormal semen parameters. Cases were allocated to treatment and observation groups, and testis volume or semen parameters were adopted as outcome measures. As a result, seven randomized controlled trials (RCTs and nonrandomized controlled trials studying bilateral testis volume or semen parameters in both treatment and observation groups were identified. Using a random effect model, mean difference of testis volume between the treatment group and the observation group was 2.9 ml (95% confidence interval [CI]: 0.6, 5.2; P< 0.05 for the varicocele side and 1.5 ml (95% CI: 0.3, 2.7; P< 0.05 for the healthy side. The random effect model analysis demonstrated that the mean difference of semen concentration, total semen motility, and normal morphology between the two groups was 13.7 × 10 6 ml−1 (95% CI: −1.4, 28.8; P = 0.075, 2.5% (95% CI: −3.6, 8.6; P= 0.424, and 2.9% (95% CI: −3.0, 8.7; P= 0.336 respectively. In conclusion, although varicocelectomy significantly improved bilateral testis volume in adolescents with varicocele compared with observation cases, semen parameters did not have any statistically significant difference between two groups. Well-planned, properly conducted RCTs are needed in order to confirm the above-mentioned conclusion further and to explore whether varicocele repair in adolescents could improve subsequently spontaneous pregnancy rates.

  18. Documento de consenso sobre el tratamiento antimicrobiano de las infecciones bacterianas odontogénicas Consensus statement on antimicrobial treatment of odontogenic bacterial infections

    Directory of Open Access Journals (Sweden)

    A Bascones Martínez

    2005-12-01

    Full Text Available Las infecciones de la cavidad bucal son un problema de salud pública frecuente y motivo constante de prescripción antibiótica; el 10% de los antibióticos se emplean para tratar este problema. Sin embargo, hasta la fecha son pocos los estudios realizados para determinar su incidencia. Asímismo, su relación con ciertas enfermedades sistémicas (cardiacas, endocrinas, etc... confiere a estas patologías una importancia vital. A pesar de la reconocida frecuencia e importancia de las infecciones odontogénicas, llama la atención la actual dispersión de criterio en varios aspectos referentes a su clasificación, terminología y recomendaciones terapéuticas. El objetivo principal de este documento, realizado con el consenso de especialistas en microbiología y odontología, es establecer unas recomendaciones útiles para todos los profesionales implicados en el manejo clínico de estas patologías. Recibe especial atención el aumento de la prevalencia de resistencias bacterianas observado durante los últimos años y, en concreto, la proliferación de cepas productoras de betalactamasas. Otro factor causal importante de la aparición de resistencias es la falta de cumplimiento terapéutico, en especial en lo que respecta a la dosis y a la duración del tratamiento. Así pues, estas patologías constituyen un problema complejo cuyo abordaje requiere la instauración de antimicrobianos de amplio espectro, con adecuados parámetros farmacocinéticos, con buena tolerancia y una posología cómoda que permita que el paciente reciba la dosis adecuada durante el tiempo necesario. Amoxicilina/ácido clavulánico a dosis altas (2000mg/ 125mg ha demostrado buenos resultados y capacidad para superar resistencias. Otros agentes como metronidazol y clindamicina, seguidos de claritromicina y azitromicina han demostrado también ser activos frente a la mayoría de los microorganismos responsables de las infecciones odontogénicas.The infection of the oral

  19. Elimination of toxicity and enhanced detection of lumpy skin disease virus on cell culture from experimentally infected bovine semen samples.

    Science.gov (United States)

    Bagla, V P; Osuagwuh, U I; Annandale, C H; Irons, P C; Venter, E H

    2006-12-01

    Lumpy skin disease virus (LSDV), a poxvirus of the genus Capripoxvirus, is shed in the semen of infected bulls. The screening of semen for infectious virus requires a sensitive diagnostic method. The isolation of the virus on cell cultures and/or the polymerase chain reaction (PCR) are sensitive diagnostic tests which may be used to screen semen for LSD viral DNA prior to artificial insemination. Although cell culture detects infectious virus and is a sensitive method, there are major difficulties in using this method due to the toxic effect of semen on the cells. The aim of this study was to find a method that decreases the toxic effect of semen and enhances the isolation of LSDV on cell culture. Semen samples from LSDV sero-negative bulls were collected and infected with a field isolate of LSDV, strain V248/93, with a titre of 6.5 log TCID50. The semen samples were treated with one of four different methods: centrifugation, serial dilution, filtration and chemical treatment with kaolin. The samples subjected to centrifugation, serial dilution and filtration were supplemented with gentamycin. Semen toxicity on cell cultures was eliminated when supernatants of semen samples centrifuged at 2000 rpm for 1, 3 and 5 min and serially diluted were used to inoculate confluent monolayer bovine dermis cells. The toxicity recorded when the pellet fractions of semen samples centrifuged for 5 min at 2000 rpm was comparable to results obtained from serially diluted samples supplemented with gentamycin. Filtration and kaolin treatment of semen samples did not remove the toxic effect.

  20. Altered expression of KLC3 may affect semen parameters

    Directory of Open Access Journals (Sweden)

    Pegah Kargar- Dastjerdy

    2016-01-01

    Full Text Available Background: KLC3 protein as a member of the kinesin light-chain protein family plays an important role in spermatogenesis, during formation of mitochondrial sheath in the mid piece of the sperm tail. Objective: This study for the first time aims to compare the expression of the KLC3 gene between fertile and infertile individuals. Materials and Methods: Semen samples were collected from 19 fertile individuals who were selected from embryo-donor volunteers and 57 infertile individuals who had abnormal sperm parameters according to world health organization criteria. Sperm parameters using computer assisted sperm analysis and the quantitative KLC3-gene expression using the real-time PCR method were measured. Results: Our results revealed a significant correlations between sperm concentration with relative expression of KLC3 only in infertile groups (r=0.45, p=0.00. A significant correlation was not found between KLC3 expression and sperm motility; however, the relative expression of KLC3 was significantly higher in asthenozoospermic compared to non-asthenozoospermic individuals. Conclusion: Low expression of KLC3 may result in improper function of midpiece, which has important function in sperm motility. The results of this study show that aberrant expression of KLC3 might be associated with phenomena like oligozoospermia and asthenozoospermia. This article is extracted from student’s thesis.

  1. Altered expression of KLC3 may affect semen parameters

    Science.gov (United States)

    Kargar- Dastjerdy, Pegah; Tavalaee, Marziyeh; Salehi, Mansoor; Falahati, Mojtaba; Izadi, Tayebeh; Nasr Esfahani, Mohammad Hossein

    2016-01-01

    Background: KLC3 protein as a member of the kinesin light-chain protein family plays an important role in spermatogenesis, during formation of mitochondrial sheath in the mid piece of the sperm tail. Objective: This study for the first time aims to compare the expression of the KLC3 gene between fertile and infertile individuals. Materials and Methods: Semen samples were collected from 19 fertile individuals who were selected from embryo-donor volunteers and 57 infertile individuals who had abnormal sperm parameters according to world health organization criteria. Sperm parameters using computer assisted sperm analysis and the quantitative KLC3-gene expression using the real-time PCR method were measured. Results: Our results revealed a significant correlations between sperm concentration with relative expression of KLC3 only in infertile groups (r=0.45, p=0.00). A significant correlation was not found between KLC3 expression and sperm motility; however, the relative expression of KLC3 was significantly higher in asthenozoospermic compared to non-asthenozoospermic individuals. Conclusion: Low expression of KLC3 may result in improper function of midpiece, which has important function in sperm motility. The results of this study show that aberrant expression of KLC3 might be associated with phenomena like oligozoospermia and asthenozoospermia. This article is extracted from student’s thesis. PMID:27141544

  2. Sperm motility patterns in Andalusian donkey (Equus asinus) semen: effects of body weight, age, and semen quality.

    Science.gov (United States)

    Dorado, J; Acha, D; Gálvez, M J; Ortiz, I; Carrasco, J J; Díaz, B; Gómez-Arrones, V; Calero-Carretero, R; Hidalgo, M

    2013-04-15

    The aims of this study were to (1) identify sperm subpopulations with specific motion characteristics in fresh Andalusian donkey ejaculates; (2) evaluate the effects of individual donkey and ejaculates within the same donkey on the distribution of the subpopulations found; and (3) explore the relationship between the age and the body weight of donkey donors, the sperm quality parameters, and the sperm subpopulations structure. Sixty ejaculates from 12 Andalusian donkeys (five ejaculates per donkey), ranging in age from 4 to 15 years, were collected. Immediately after collection, sperm characteristics (volume, sperm concentration, objective sperm motility, and sperm morphology) were assessed. Donkeys were evaluated for body weight. Significant (P donkeys and the pH (r = -0.52), sperm motility (percentage of motile spermatozoa: r = -0.31; percentage of progressive motile spermatozoa: r = -0.34), and total sperm abnormalities (r = 0.38). The correlations of the age with the measures of semen quality were low and not significant (P > 0.05). A multivariate clustering procedure separated 65,342 motile spermatozoa into four subpopulations: subpopulation 1, consisting of slow and nonprogressive spermatozoa (15.4%), subpopulation 2, consisting of moderately slow but progressive spermatozoa (35.9%), subpopulation 3, consisting of highly active but nonprogressive spermatozoa (18.5%), and subpopulation 4, consisting of highly active and progressive spermatozoa (30.2%). The distribution of these subpopulations varied significantly (P donkey, the ejaculate of the same donkey, the total motility, and the overall sperm concentration. Our results show the existence of four well-defined motile sperm subpopulations in Andalusian donkey ejaculates, and suggest a high heterogeneity in the ejaculate structure in donkey. The relationship between the distribution of the sperm subpopulations and individual donkey, total motility, and sperm concentration shows that the spermatozoa of each

  3. Simple and effective methods of freezing capercaillie (Tetrao urogallus L. semen.

    Directory of Open Access Journals (Sweden)

    Artur Kowalczyk

    Full Text Available A continuous decline in the number and range of capercaillie (Tetrao urogallus L. in many European countries can be observed, mostly due to habitat destruction by human activity, unecological forestry management, and increased density of natural predators. Ex situ in vitro gene banks provide a unique opportunity to preserve the genetic material for future generations. Simple and effective cryopreservation methods for capercaillie semen are discussed. Semen was collected from seven males kept in the Capercaillie Breeding Centre at Forestry Wisła in Poland. Within five minutes after collection, ejaculates were diluted with EK diluent, then divided into two parts, and subjected to two freezing procedures: in pellets and in straws. In fresh semen, ejaculate clearness, viscosity, color and volume, as well as sperm concentration, motility and morphology, were evaluated, while in frozen-thawed semen only motility and morphology of sperm were determined. Fertilizing ability of thawed semen was examined for samples frozen in straws. Significant (P<0.05 differences between individual males were found in relation to the majority of fresh semen traits: ejaculate volume averaged 102.1 µL (varying from 49.0 to 205.0; average sperm concentration was 632.5 x 10⁶ mL⁻¹ (178.8-1257.1; percentage of live normal cells varied from 39.2 to 70.3% (58.7% on an average; percentage of motile cells ranged from 76.0 to 85.7% and motility parameters were male dependent, as well. Both cryopreservation methods had a negative effect on morphology and motility of frozen-thawed semen; however, the straw method yielded 60.7% and the pellet method 42.5% of live cells in total in thawed semen (P<0.05, while the number of live normal (intact cells was similar (22.4 and 22.2%, respectively. Egg fertility varied between 77.8 and 91.7% (average 84.4%. Both freezing procedures seem to be effective in obtaining acceptable viability and high fertilizing potency of thawed sperm and

  4. Effect of dietary selenium and vitamin E on ganders' response to semen collection and ejaculate characteristics.

    Science.gov (United States)

    Jerysz, Anna; Lukaszewicz, Ewa

    2013-06-01

    Compared to other domestic bird species, geese exhibit the lowest reproductive efficiency (poor semen quality, low egg production, and poor fertility and hatchability rates). From an economic perspective, it is a necessity of improve these reproductive traits. Studies have demonstrated that the essential trace element-selenium-plays key roles in testicular development and the maintenance of spermatogenesis. The aim of the present study was to determine the effect of feed supplementation with organic selenium and vitamin E on ganders' response to manual semen collection and semen quality. Sixteen 3-year-old White Koluda ganders were randomly divided into two groups. The control group was provided commercial feed while the experimental group was provided with the same commercial feed supplemented with selenium (0.3 mg/kg) and vitamin E (100 mg/kg). The response of individual ganders from both groups to manual semen collection and the quality of the semen collected were evaluated. The supplements increased (P ≤ 0.05) the frequency and decreased the time interval of a complete ejaculatory response of the ganders to manual semen collections (82.7 % supplement vs. 73.5 % control). Males from the supplemented group had significantly higher (P ≤ 0.01; P ≤ 0.05) ejaculate volumes, sperm concentrations, and percentages of viable sperm and lower percentages of immature sperm (spermatids). Lipids peroxidation, expressed in terms of the malondialdehyde concentration, was lower (P ≤ 0.01) in semen of the supplemented group (0.172 nmol/50 × 10(6)) as compared to the controls (0.320 nmol/50 × 10(6)). Moreover, the duration of the reproductive period of the ganders in the experimental group was 1 week longer. The results show that supplemental dietary selenium and vitamin E improved both the ganders' response to manual semen collection and semen quality. We conclude that such feed supplementation could lead to greater economic benefits

  5. Artificial insemination field data on the use of sexed and conventional semen in nulliparous Holstein heifers.

    Science.gov (United States)

    Healy, A A; House, J K; Thomson, P C

    2013-03-01

    This study investigated conception rates and other reproductive outcomes achieved with artificial insemination (AI) of nulliparous Holstein heifers using sexed and conventional semen in a commercial Australian dairy herd in central western New South Wales from January 2004 to April 2009. Retrospective data from on-farm records of 9,870 inseminations of 4,456 heifers were analyzed using several mixed models to assess the effect of temperature and humidity surrounding breeding, insemination sire, artificial insemination technician, service number, and heifer weight and age at breeding on reproductive traits (conception rates, sex ratios, gestation length, and abortion and stillbirth rates). Semen was used from 15 sexed sires and 41 unsexed sires. Sexed semen was primarily used at first and second service. Empirical conception rates of 31.6 and 39.6% were achieved for sexed and unsexed semen respectively, whereas model-based predictions were lower, at 21.3 and 32.1%. Conception rates were significantly affected by insemination sire, sex-sorting, heifer age at breeding, temperature and humidity surrounding breeding, service number, and AI technician. Sexed semen yielded 86% heifers, compared with 48% for conventional semen. Significant predictors of calf sex included semen sexing, gestation length, and insemination sire. Twinning rate was high, at 3.6% for both semen types, and gestation length and heifer weight at breeding were significant predictors of twinning. Abortion rates for sexed and unsexed conceptions were similar at 6.1 and 6.5%, respectively, and were affected by heifer age at breeding. Stillbirth rate was affected by calf sex, twinning, gestation length, and AI technician; semen sorting, age at breeding, and temperature and humidity were marginally significant predictors. No abnormalities were observed in the development of offspring, except for a marginally higher stillbirth rate for sexed calves, a finding that needs further investigation. Many

  6. Recomendaciones para el manejo de la faringoamigdalitis aguda del adulto

    Directory of Open Access Journals (Sweden)

    Josep M. Cots

    2015-10-01

    Full Text Available La faringoamigdalitis aguda (FAA en el adulto es una de las enfermedades infecciosas más comunes en la consulta del médico de familia. La etiología más frecuente es viral. Dentro de la etiología bacteriana, el principal agente responsable es Streptococcus pyogenes o estreptococo β-hemolítico del grupo A (EBHGA, causante del 5-30% de los casos. En el manejo diagnóstico, las escalas de valoración clínica para predecir la posible etiología bacteriana, son una buena ayuda para seleccionar a qué pacientes se deben practicar las técnicas de detección rápida de antígeno estreptocócico. Es conocido que, en general, sin estas técnicas se tiende al sobrediagnóstico de FAA estreptocócica, con la consiguiente prescripción innecesaria de antibióticos, muchas veces de amplio espectro. Así, con el manejo de las escalas y la técnica de diagnóstico rápido, elaboramos los algoritmos de manejo de la FAA. Los objetivos del tratamiento son acelerar la resolución de los síntomas, reducir el tiempo de contagio y prevenir las complicaciones supurativas locales y no supurativas. Los antibióticos de elección para el tratamiento de la FAA estreptocócica son penicilina y amoxicilina. La asociación de amoxicilina y clavulánico no está indicada en el tratamiento inicial en la infección aguda. Los macrólidos tampoco son un tratamiento de primera elección; su uso debe reservarse para pacientes con alergia a la penicilina. Es importante en nuestro país adecuar tanto el diagnóstico de la FAA bacteriana y la prescripción de antibióticos a la evidencia científica disponible. La implantación de protocolos de actuación en las farmacias comunitarias puede ser de utilidad para identificar y cribar los casos que no requieran tratamiento antibiótico.

  7. SEMEN CHARACTERISTICS OF CROSSBRED (FRIESIAN X SAHIW AL) AND SAHIWAL YOUNG BULLS MAINT AINED UNDER SUB-TROPICAL CONDITIONS OF PUNJAB

    OpenAIRE

    Ahsan-ul-Haq, R.H. Mirza and I.A. Zahid

    2003-01-01

    Comparative studies of semen characteristics were carried out in 50% crossbred (Friesian X Sahiwal) and purebred Sahiwal young bulls maintained under sub-tropical conditions at Research Institute of Physiology of Animal Reproduction, Bhunikey and Semen Production Unit. Qadirabad, respectively. Semen was collected on weekly intervals for a period of one year. The semen characteristics (mean+SE), i.e., volume per ejaculate (ml), mass motility (0-5 score), individual motility of spermatozoa (%),...

  8. Use of commercial extenders and alternatives to prevent sperm agglutination for cryopreservation of brown bear semen.

    Science.gov (United States)

    Gomes-Alves, S; Alvarez, M; Nicolas, M; Lopez-Urueña, E; Martínez-Rodríguez, C; Borragan, S; de Paz, P; Anel, L

    2014-08-01

    The objective of this study was to evaluate different bovine and canine commercial semen extenders for cryopreservation of brown bear ejaculates and the effect of semen collection directly into extender on sperm agglutination. Semen samples were obtained by electroejaculation from 13 adult males. In experiment 1, eleven ejaculates from eight bears were used to evaluate Bioxcell and Andromed as extenders, whereas in experiment 2, nine ejaculates from six bears were used to evaluate Triladyl canine, CaniPro, and Extender 2 as extenders. An extender specifically developed for brown bears (Test-Tris-fructose-egg yolk-glycerol, TTF-ULE/bear) served as a control extender in both experiments. After thawing, total and progressive sperm motility and sperm viability were greater (P agglutination in fresh samples (score 0.5 ± 0.2 vs. 1.8 ± 0.4 in diluted and control samples, respectively) with no effect on pre-freeze and post-thawing semen quality. In conclusion, TTF-ULE/bear is the most suitable extender for brown bear semen cryopreservation, but comparable results can be obtained with the commercial extender Andromed. In addition, collection of ejaculates directly in TTF-H extender decreases sperm agglutination in fresh samples.

  9. Genetic parameters and breeding values for semen characteristics in Hanoverian stallions.

    Science.gov (United States)

    Labitzke, D; Sieme, H; Martinsson, G; Distl, O

    2014-08-01

    The objectives of this study were to show whether semen traits of 30 Hanoverian stallions regularly used in AI may be useful for breeding purposes. Semen characteristics were studied using 15 149 ejaculates from 30 Hanoverian stallions of the State Stud Celle of Lower Saxony. Semen samples were collected between 2005 and 2009. Traits analysed were gel-free volume, sperm concentration, total and motile sperm number and progressive motility. A linear multivariate animal model was employed to estimate heritabilities and permanent environmental variances for stallions. The same model was used to predict breeding values for all traits simultaneously. Heritabilities were high for gel-free volume (h(2) = 0.43) and moderate for total number of sperm (h(2) = 0.29) and progressive motility (h(2) = 0.20). Gel-free volume, sperm concentration and total number of sperm were genetically negatively correlated with progressive motility. The effect of the permanent environment for stallions accounted for 9-55% of the trait variance. The total variance among stallions explained 37-69% of the trait variance. The average reliabilities of the breeding values were 0.43-0.76 for the 30 Hanoverian stallions. In conclusion, the study could demonstrate large effects of stallions, routinely employed in a breeding programme, on semen characteristics analysed here. We could demonstrate that estimated breeding values (EBV) with sufficient high reliabilities can be predicted using data from these stallions and these EBV are useful in horse breeding programmes to achieve genetic improvement in semen quality.

  10. Assessment of semen quality in Swamp Buffalo AI Bulls in Thailand

    Directory of Open Access Journals (Sweden)

    S. Koonjaenak

    2010-02-01

    Full Text Available Characteristic of Thai swamp buffalo bulls semen used for artificial insemination (AI in Thailand, aspects relevance in freezing and thawing of semen are review. Semen and sperm characteristics were evaluated included sperm count, motility (assessed subjectively and by CASA, morphology (using phase-contrast light microscopy and SEM, plasma membrane integrity (PMI (using a hypo-osmotic swelling test [HOST] and SYBR- 14/propidium iodide [PI], plasma membrane stability (PMS (using Annexin-V/PI and deoxyribonucleic acid (DNA integrity (using SCSA and flow cytometry [FCM]. The average ejaculate volume was about 3.0–4.0 mL, with good viability (PMI measured by the HOST and motility (>65% and >70%, respectively. Sperm concentration ranged from 1.1 to 1.2 billion/mL, being also affected by bull age. Whereas semen quality (including sperm output, pH and initial sperm motility did not differ between the seasons. Few spermatozoa (<15%/ ejaculate had abnormal morphology with abnormalities resembling those in other bovidae. In FT semen, PMI (using SYBR-14/PI and PMS were highest in winter. Across seasons, ~50% of post-thaw spermatozoa depicted linear motility, a proportion that decreased to ~35% during incubation (38oC for 60 minutes, without marking any seasonal difference. The sperm DNA was hardly damaged (with <3% fragmentation, expressed as DNA fragmentation index [DFI], among seasons.

  11. Potential factors affecting semen quality in the Asian elephant (Elephas maximus

    Directory of Open Access Journals (Sweden)

    Pongsopavijitr Pornsawan

    2008-03-01

    Full Text Available Abstract Background One of the major obstacles in using artificial insemination to manage genetics of elephant population in captivity is the large variations in semen quality among ejaculates within the same and among individuals. The objectives of this study were to determine the influences of (1 age (2 seasonality (3 and circulating testosterone (SrTest, triiodothyronine (SrT3 and tetraiodothyronine (SrT4, as well as seminal (4 testosterone (SpTest, zinc (SpZn and protein (SpTP on semen quality in the Asian elephant Methods Analyses, including motility, viability and morphology were performed in semen samples collected twice monthly from 13 elephant bulls (age range, 10-to 72-years by manual stimulation between July 2004 and June 2005. Serum samples obtained monthly were assessed for SrTest, SrT3, SrT4, and seminal plasma samples were evaluated for, SpTest, SpZn and SpTP. Results The highest semen quality was observed at age 23 to 43 years. Percentages of progressive motility and viable sperm were lowest at age 51 to 70 years (P Conclusion This study indicates that age and seasonality had influence on semen characteristics in the Asian elephant. The knowledge obtained in this study will improve our understanding of the reproductive biology of this species.

  12. EFFECT OF DIFFERENT CRYOPROTECTIVE AGENTS ON SKIM MILK AND DIMITROPOULUS EXTENDER FOR STALLION SEMEN CRYOPRESERVATION

    Directory of Open Access Journals (Sweden)

    R.I. Arifiantini

    2014-10-01

    Full Text Available s to assess different CPAs on stallion semen cryopreservation. Skim milk (SM and Dimitropoulos(DV were the extenders used in this study; each was added by glycerol (Gly, combination of ethyleneglycol-glycerol (EG+Gly or dimethilformamide (DMF. Each semen sample was evaluated and dividedequally into six tubes; semen in the three tubes was diluted 1:1 with (SM, while in the remaining tubesthe semen was diluted 1:1 by DV. After being diluted, all tubes were centrifuged at 1006xg for 10minutes. The supernatan discarded, the pellet was rediluted by SM trehalosa or DV trehalose, and addedby G, EG+Gly, or DMF to reach the final sperm concentration of 200x106/ml. The extended semen wasindividually packed in 0.3 ml minitube, equilibrated at 4oC for 2 hours, frozen in liquid nitrogen vaporfor 10 minutes, and then was stored in liquid nitrogen container at -196 oC. After 24 hours, the semenwas thawed at 37 oC for 30 second. There were no significantly different (p>0.05 on the percentages ofmotile and viable sperm in SMT (21.7% and 43.4%, respectively compared with those extended withDV T extender (26.9% and 50.8%, respectively. DMF demonstrated better results as CPA compared tothe others; and DVTDMF combination had the best protection during cryopreservation in this study.

  13. HPV prophylactic vaccination in males improves the clearance of semen infection.

    Science.gov (United States)

    Foresta, Carlo; Garolla, Andrea; Parisi, Saverio; Ghezzi, Marco; Bertoldo, Alessandro; Di Nisio, Andrea; De Toni, Luca

    2015-10-01

    •We evaluated whether seroconversion in males influences HPV semen infection.•Naturally seroconverted patients showed reduced prevalence of HPV semen infection.•Naturally seroconverted patients also showed virtual absence of HPV multiple infection.•Prophylactic HPV vaccination induced clearance within 12 months from recruitment.•Seroconversion represents a key process involved in the clearance of the HPV.Available prophylactic vaccinations are considered of protective value for genital condyloma and precancerous lesions in female, but cost-effectiveness of the use of HPV vaccine in males is largely underinvestigated. HPV detection in semen is also an emerging problem in couples eligible for assisted reproduction techniques, since persistent infections are not compatible with repeated 6-months counselling-cycles to allow any spontaneous clearance of the virus in older infertile couples.In this study, we provide evidence that the development of seroconversion in human males affected by HPV infection in the genito-urinary tract, detected by HPV-DNA presence in the semen, has beneficial effects on the clearance of a viral load. Moreover, administration of prophylactic vaccination to HPV infected-seronegative patients induced seroconversion within 6 months from the first dosage administration, achieving 10 folds-higher antibody titre compared to natural seroconversion. If vaccine administration ameliorates the clearance of HPV semen infection, this could be a potential benefit to overcome fertility problems related to persistent HPV infections in males, after an obvious cost-effectiveness analysis.

  14. Semen Quality in Infertile Men with a History of Unilateral Cryptorchidism

    Institute of Scientific and Technical Information of China (English)

    P. Tzvetkova; Wei-Jie ZHU; D. Tzvetkov

    2004-01-01

    Objective To evaluate semen quality in infertile men with a history of unilateral cryptorchidismMethods Semen samples were obtained from 47 infertile men with a history of unilateral cryptorchidism. Semen analysis and membrane integrity including both eosin Y stain and hyo-osmotic swelling tests were performed using standard procedures. All men were divided into three groups according to their age at surgery oforchidopexy as follows: group A, 2-7 years (n=23), group B, 8-13 years (n=14) and group C,14-17 years (n=10).Results There was wide range for sperm count and motility for all the subjects studied.Majority of men had high percentage of abnormal sperm morphology. Among the three groups of men, group A and B had significantly higher sperm count, motility and membrane integrity than that of group C. Group C also had the highest frequency of oligoasthenoteraozoospermia (50%) than group A (17%) and group B (35%).Conclusion Semen quality of men with a history of unilateral cryptorchidism appears to be related to their age of performing orchidopexy. Surgery at early age of childhood may improve spermatogenesis which results in better semen quality in adulthood.

  15. Effects of Three Different Diluents on Quality of Boar Semen Stored at 17℃

    Institute of Scientific and Technical Information of China (English)

    Hu Shan; Zhang Xiao-gang; Han Cong; Wei Shuai-yi; Xie Dong-qi; Du Ren-rang; Hu Jian-hong

    2015-01-01

    To investigate the effects of different diluents on the quality of the boar semen stored at 17℃, and assess the relationship between sperm motility and the relative levels of enzymes, three commercial diluents (DiluentⅠ, DiluentⅡand DiluentⅢ) and three boar breed semens (Yorkshire, Landrace and Duroc) were utilized. The sperm motility, effective survival time, survival index, catalase (CAT), the total anti-oxidative capacity (T-AOC) and malondialdehyde (MDA) levels were evaluated. The results showed that there were significant interaction effects between diluents and breeds on the boar sperm motility (P0.05). All of the parameters varied significantly with the increase of the storage time (P<0.001). The survival time increased 12.9% in Yorkshire boar semen diluted with DiluentⅢ than with DiluentⅡ, while the survival time increased 6.6% in Landrace boar semen diluted with DiluentⅡ than with DiluentⅢ. Both CAT and T-AOC levels were significantly positive correlated with sperm motility in all the three boar breeds (P<0.001), while MDA levels were significantly negative correlated with sperm motility (P<0.001). These results indicated that DiluentⅢ and DiluentⅡwere the optimal commercial diluents for Yorkshire and Landrace boar semen stored at 17℃, respectively.

  16. Temperature management during semen processing: Impact on boar sperm quality under laboratory and field conditions.

    Science.gov (United States)

    Schulze, M; Henning, H; Rüdiger, K; Wallner, U; Waberski, D

    2013-12-01

    Freshly collected boar spermatozoa are sensitive to a fast reduction in temperature because of lipid phase transition and phase separation processes. Temperature management during semen processing may determine the quality of stored samples. The aim of this study was to evaluate the influence of isothermic and hypothermic semen processing protocols on boar sperm quality under laboratory and field conditions. In the laboratory study, ejaculates (n = 12) were first diluted (1:1) with Beltsville Thawing Solution (BTS) at 32 °C, then processed either with isothermic (32 °C) or hypothermic (21 °C) BTS, stored at 17 °C, and assessed on days 1, 3, and 6. Temperature curves showed that 150 minutes after the first dilution, semen doses of both groups reached the same temperature. Two-step hypothermic processing resulted in lower sperm motility on days 1 and 6 (P sperm on days 3 and 6 (P boar semen compared with isothermic dilution and that the type of semen extender affects the outcomes.

  17. EFFECT OF ANTIBIOTICS IN EXTENDER ON FERTILITY OF LIQUID BUFFALO BULL SEMEN

    Directory of Open Access Journals (Sweden)

    S. AKHTER, M. SAJJAD, S. M. H. ANDRABI1, N. ULLAH1 AND M. QAYYUM

    2007-01-01

    Full Text Available This study was carried out to determine if a new antibiotic combination comprising of gentamycin, tylosin and linco-spectin (GTLS in extender is suitable for improvement in fertility of liquid buffalo bull semen through artificial insemination (AI. Two consecutive ejaculates per week (4 weeks were collected from three Nili-Ravi buffalo bulls of known fertility by using artificial vagina. The pooled ejaculates were split-sampled and diluted with skimmed milk extender (37oC; 10x106 motile spermatozoa/ml containing either SP (streptomycin 1000 μg/ml and penicillin 1000 iu/ml or GTLS (gentamycin 500 μg/ml, tylosin 100 μg/ml, lincomycin 300 μg/ml, and spectinomycin 600 μg/ml. Liquid semen was stored at 5°C for seven days. Fertility, based on 90-days first service pregnancy rate, was determined under field conditions. The fertility rates for SP-based vs. GTLS-containing liquid semen of buffalo bull were 58.55 and 60.00%, respectively, the difference was non significant. The fertility rates also did not differ (P>0.05 due to antibiotics at different days of storage of liquid semen at 5oC. In conclusion, GTLS, in skimmed milk extender compared to SP, did not significantly improve the fertility of chilled buffalo bull semen.

  18. Low lead environmental exposure alters semen quality and sperm chromatin condensation in northern Mexico.

    Science.gov (United States)

    Hernández-Ochoa, Isabel; García-Vargas, Gonzalo; López-Carrillo, Lizbeth; Rubio-Andrade, Marisela; Morán-Martínez, Javier; Cebrián, Mariano E; Quintanilla-Vega, Betzabet

    2005-01-01

    We evaluated environmental-lead (Pb) effects on semen quality and sperm chromatin, considering Pb in seminal fluid (PbSF), spermatozoa (PbSpz), and blood (PbB) as exposure biomarkers in urban men (9.3 microg/dL PbB). Several individuals (44%) showed decreases in sperm quality; sperm concentration, motility, morphology and viability associated negatively with PbSpz, whereas semen volume associated negatively with PbSF. Multiple linear regression estimated PbSF and PbSpz thresholds for alterations in semen quality. Forty-eight percent of samples showed high values of nuclear chromatin condensation (NCD) positively associated with PbSF and zinc in spermatozoa (ZnSpz). ZnSpz values were higher than in fertile men. These results suggest that Pb may affect sperm chromatin by altering sperm Zn availability. PbB was not associated with semen quality or NCD, suggesting that Pb in semen compartments assesses better the amount of Pb in the reproductive tract; therefore, these are better biomarkers to evaluate toxicity at low Pb-exposure levels.

  19. Zinc and iron concentration and SOD activity in human semen and seminal plasma.

    Science.gov (United States)

    Marzec-Wróblewska, Urszula; Kamiński, Piotr; Lakota, Paweł; Szymański, Marek; Wasilow, Karolina; Ludwikowski, Grzegorz; Kuligowska-Prusińska, Magdalena; Odrowąż-Sypniewska, Grażyna; Stuczyński, Tomasz; Michałkiewicz, Jacek

    2011-10-01

    The aim of the present study was to measure zinc (Zn) and iron (Fe) concentration in human semen and superoxide dismutase (SOD) activity in seminal plasma and correlate the results with sperm quality. Semen samples were obtained from men (N = 168) undergoing routine infertility evaluation. The study design included two groups based on the ejaculate parameters. Group I (n = 39) consisted of males with normal ejaculate (normozoospermia), and group II (n = 129) consisted of males with pathological spermiogram. Seminal Zn and Fe were measured in 162 samples (group I, n = 38; group II, n = 124) and SOD activity in 149 samples (group I, n = 37; group II, n = 112). Correlations were found between SOD activity and Fe and Zn concentration, and between Fe and Zn concentration. SOD activity was negatively associated with volume of semen and positively associated with rapid progressive motility, nonprogressive motility, and concentration. Negative correlation was stated between Fe concentration and normal morphology. Mean SOD activity in seminal plasma of semen from men of group I was higher than in seminal plasma of semen from men of group II. Fe concentration was higher in teratozoospermic males than in males with normal morphology of spermatozoa in group II. Our results suggest that Fe may influence spermatozoa morphology.

  20. Determination of Sperm Sex Ratio in Bovine Semen Using Multiplex Real-time Polymerase Chain Reaction.

    Science.gov (United States)

    Khamlor, Trisadee; Pongpiachan, Petai; Sangsritavong, Siwat; Chokesajjawatee, Nipa

    2014-10-01

    Gender selection is important in livestock industries; for example, female calves are required in the dairy industry. Sex-sorted semen is commonly used for the production of calves of the desired gender. However, assessment of the sex ratio of the sorted semen is tedious and expensive. In this study, a rapid, cost effective and reliable method for determining the sex ratio was developed using a multiplex real-time polymerase chain reaction (PCR) assay. In this assay, the X and Y chromosome-specific markers, i.e., bovine proteolipid protein (PLP) gene and sex-determining region Y (SRY) were simultaneously quantified in a single tube. The multiplex real-time PCR assay was shown to have high amplification efficiencies (97% to 99%) comparable to the separated-tube simplex real-time PCR assay. The results obtained from both assays were not significantly different (p>0.05). The multiplex assay was validated using reference DNA of known X ratio (10%, 50%, and 90%) as templates. The measured %X in semen samples were the same within 95% confidence intervals as the expected values, i.e., >90% in X-sorted semen, PCR assay as shown in this study can thus be used to assess purity of sex-sorted semen.

  1. Effect of smoking on semen quality of infertile men in Shandong, China

    Institute of Scientific and Technical Information of China (English)

    Jin-PingZHANG; Qing-YuMENG; QiangWANG; Lei-JiaZHANG; Yi-LongMAO; Zhuo-XiangSUN

    2000-01-01

    Aim: To study the effect of smoking on the semen quality in infertile men in Shandong Province, China. Methods: Adult non-drinker males attending the infertility clinic, including 110 non-smokers and 191 smokers, were recruited for the study. Sixty-one fertile, non-smoker and non-drinker males, who had one or more children, served as the controls. The smokers were divided into subgroups according to the amount and duration of smoking. Semen parameters (semen volume and sperm density, viability, motility, and morphology) were examined and seminal plasma contents of Zn , Cu and superoxide dismutase (SOD) determined. Results: The semen volume and acidity, and the sperm density, viability and forward progression, as well as the seminal plasma contents of Zn, Cu and SOD were much lower in the medium, heavy and long-term smokers than in the non-smokers (P < 0.01). The sperm density, viability and forward progression, and the seminal plasma Zn, Cu and SOD levels were negatively correlated with the amount and duration of cigarette smoking (P < 0.01). Conclusion: Medium, heavy and long-term smoking adversely affected the semen quality in a population of men visiting the infertility clinic in Shandong, China.

  2. Computer assisted semen analysis for quantification of motion characteristics of bull sperm during cryopreservation cycle

    Directory of Open Access Journals (Sweden)

    M. N. Sundararaman

    Full Text Available Aim: The study was undertaken to quantify and to analyze the changes in the motion characteristics of bull spermatozoa during various stages of cryopreservation cycle. Materials and Methods: Using computer assisted semen analysis (CASA technique, 26 ejaculates, collected from two Jersey bulls were analyzed for motility, head behaviour and swimming pattern of spermatozoa on dilution, pre-freeze and post-thaw stages of cryopreservation. French straw technique was employed for deep-freezing of semen using liquid nitrogen. Results: Equilibration of diluted semen at 5 C has significantly (P< 0.01 reduced sperm motility, progressive motility, path velocity, and progressive velocity. Beat cross frequency was also affected significantly (P<0.05 by equilibration. Freezing and thawing processes drastically affected all the motility, velocity and head behaviour characteristics (P< 0.01. Conclusion: CASA facilitate objective evaluation sperm motion characteristics. Adoption of CASA technique has the potential for improvements in evaluation of semen thereby the quality of frozen semen for fertility can be enhanced. [Vet World 2012; 5(12.000: 723-726

  3. Embryo production and possible species preservation by nuclear transfer of somatic cells isolated from bovine semen.

    Science.gov (United States)

    Liu, Jie; Westhusin, Mark; Long, Charles; Johnson, Gregory; Burghardt, Robert; Kraemer, Duane

    2010-12-01

    Somatic cells in semen are a potential source of nuclei for nuclear transfer to produce genetically identical animals; this is especially important when an animal has died and the only viable genetic material available is frozen semen. Usefulness of somatic cells obtained from fresh (cultured) and frozen (isolated, not cultured) bovine semen for nuclear transfer was evaluated. Twelve ejaculates were collected from nine bulls representing three breeds: Charolais, Brahman, and crossbred Rodeo bull. All samples were processed immediately and cell growth was obtained from seven of the twelve ejaculates (58.3%). Cells from three bulls (with the best growth rates) were evaluated by optical microscopy and used in cloning experiments. In culture, these cells exhibited classic epithelial morphology and expressed cytokeratin and vimentin, indicating they were of epithelial origin. When cells from the three bulls were used as donor cells, 15.9% (18/113), 34.5% (29/84), and 14.4% (13/90) of the fused embryos developed into blastocysts, respectively. Of the blastocyst stage embryos, 38.9% (7/18), 72.4% (21/29), and 61.5% (8/13) hatched, respectively. Somatic cells isolated (not cultured) from frozen bovine semen were also used in the cloning experiments. Although cleavage occurred, no compact morulae or blastocysts were obtained. In conclusion, epithelial cell growth was obtained from fresh bovine ejaculates with relatively high efficiency. Somatic cells from semen can be used as nucleus donors to produce cloned blastocyst-stage embryos.

  4. Evaluation of sperm DNA damage in bulls by TUNEL assay as a parameter of semen quality.

    Science.gov (United States)

    Takeda, Kumiko; Uchiyama, Kyoko; Kinukawa, Masashi; Tagami, Takahiro; Kaneda, Masahiro; Watanabe, Shinya

    2015-01-01

    Sperm DNA damage affects the conception rate resulting from human assisted reproduction technology. The objective of this study was to adapt the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay to provide a quality parameter for bull semen based on the detection of sperm DNA damage. Fresh semen was collected from two Japanese Black bulls (A, B) several times over the course of a year, and the percentage of TUNEL-positive spermatozoa (sperm TUNEL index) was determined. Individual differences in semen were detected using the sperm TUNEL index in these bulls (P bulls with a conception rate lower than 10%) and Holstein (n = 34) bulls were analyzed. The average sperm TUNEL index and conception rate resulting from artificial insemination (AI) were 4.7% and 55.7% for Japanese Black, and 4.9% and 39.5% for Holstein, respectively. A weak negative correlation between sperm TUNEL index and conception rate was observed in Holstein bulls (P bulls with more than 10% sperm TUNEL index were studied, and these samples showed low sperm viability. However, semen resulting in a very low conception rate did not have a high sperm TUNEL index. Although it would be difficult to predict a low conception rate resulting from AI using the sperm TUNEL index alone, the index can be used as an additional parameter to provide a more comprehensive description of semen quality.

  5. Prognosis of bacterial meningitis in children Prognóstico de meningite bacteriana em crianças

    Directory of Open Access Journals (Sweden)

    Irenio Gomes

    1996-09-01

    Full Text Available We studied the incidence and prognosis of acute neurologic complications in 281 children under 13 years of age with a diagnosis of acute bacterial meningitis. All the patients were examined daily by the same group of neurologists, using a standardized neurological examination. Patients with signs of encephalic lesions, unsatisfactory response to antibiotics or decreased level of consciousness were submitted to brain computer tomography. The overall lethality rate was 20.3% and cases whose causative agent was identified presented a higher lethality rate (23.7% than those in which the agent was not found. The most important neurological abnormalities were meningeal signs (88.3% followed by decreased consciousness (47.7%, irritability (35.2%, seizures (22.4%, fontanel bulging (20.6% and cranial nerve palsy (14.2%. Seizures, cranial nerve palsy and the absence of meningeal signs were related to higher rates of lethality. Diminished consciousness, seizures, subdural effusion, abscess and hydrocephalus were the most important complications, respectively. We can conclude that acute bacterial meningitis continues to be an important health problem in developing countries and that public health measures will be necessary to minimize the impact of sequelae and reduce the mortality rate in children with that pathology.Para estudar os fatores prognósticos e incidência de complicações em meningite bacteriana, 281 crianças (idade abaixo de 13 anos com quadro clínico e do LCR compatíveis com esse diagnóstico foram acompanhadas, durante o internamente, através de ficha de avaliação padrão. Aquelas que apresentaram sinais de lesão encefálica, resposta inadequada ao tratamento ou diminuição do nível de consciência, com Glasgow < 7, foram submetidas a tomografia computadorizada de crânio. A taxa de letalidade global foi 20,3%, sendo maior quando o agente etiológico foi identificado (23,7%. As alterações neurológicas mais encontradas foram

  6. CRIOCONSERVACIÓN DE SEMEN EN PECES: EFECTOS SOBRE LA MOVILIDAD ESPERMÁTICA Y LA FERTILIDAD.

    Directory of Open Access Journals (Sweden)

    JOSÉ GREGORIO MARTÍNEZ

    2010-01-01

    Full Text Available La crioconservación de semen de peces, como de otras especies, presenta aún efectos que disminuyen la calidad espermática y comprometen directamente la capacidad de la célula para participar exitosamente en los procesos de fertilización y desarrollo embrionario. Características como movilidad y capacidad de fertilización del espermatozoide son consideradas criterios de calidad que permiten medir el éxito o fracaso del proceso, pues se consideran variables integradoras, siendo indicadores que dependen no de un solo factor sino de la estabilidad y bienestar del conjunto de estructuras, enzimas y compuestos funcionales subcelulares que dan lugar a estas características espermáticas. Daños en la membrana (adenilato ciclasa, canales iónicos, agrupamiento de otras proteínas, entre otras y su implicación en la ruta de señalización que da lugar a la activación espermática, degradación del ATP, fragmentación del ADN nuclear y mitocondrial (genoma, degradación de enzimas Kinasas y otras proteínas citosólicas (proteoma son considerados hoy día como algunos de los factores moleculares que más se afectan durante la crioconservación y que disminuyen ostensiblemente la capacidad fertilizante y la movilidad del espermatozoide en los peces. Propuestas sobre los mecanismos moleculares por los cuales se interrelacionan y actúan estos factores subcelulares como consecuencia de la crioconservación, son algunos de los temas tratados en la presente revisión. Comprender los principios y factores que están involucrados en el origen de dichos daños, permitirá mejorar los procesos de crioconservación, haciéndolos menos nocivos y más eficientes.

  7. Effects of different concentrations of sucrose or trehalose on the post-thawing quality of cattle bull semen

    Directory of Open Access Journals (Sweden)

    Reda I. El-Sheshtawy

    2015-03-01

    Conclusion: It is finally concluded that the addition of 50–100 mM trehalose or sucrose/L to TCYF have a beneficial effect in chilling diluted bull semen, while the use of 50 mM trehalose or 50–100 mM sucrose had their benefits on freezing-thawing of extended semen.

  8. Excretion of bovine herpesvirus 1 in semen is detected much longer by PCR than by virus isolation

    NARCIS (Netherlands)

    Engelenburg, van F.A.C.; Schie, van F.W.; Rijsewijk, F.A.M.; Oirschot, van J.T.

    1995-01-01

    To compare the sensitivities of PCR and virus isolation and to examine the course of virus excretion in semen, we intrapreputially inoculated eight bulls with bovine herpesvirus 1 (BHV1) and used two bulls as sentinels. From these bulls, we collected a large panel of semen samples during 65 days pos

  9. Relation between semen quality and fertility: a population-based study of 430 first-pregnancy planners

    DEFF Research Database (Denmark)

    Bonde, J.P.E.; Ernst, E.; Jensen, Tina Kold

    1998-01-01

    Semen analysis is part of the routine assessment of infertile couples. WHO defines a sperm concentration above 20x10(6) per mL seminal fluid as normal. We studied the association between semen quality and the probability of conception in a single menstrual cycle in Danish couples with no previous...

  10. Sensitive simultaneous detection of seven sexually transmitted agents in semen by multiplex-PCR and of HPV by single PCR.

    Directory of Open Access Journals (Sweden)

    Fabrícia Gimenes

    Full Text Available Sexually transmitted diseases (STDs may impair sperm parameters and functions thereby promoting male infertility. To date limited molecular studies were conducted to evaluate the frequency and type of such infections in semen Thus, we aimed at conceiving and validating a multiplex PCR (M-PCR assay for the simultaneous detection of the following STD pathogens in semen: Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, Herpes virus simplex (HSV -1 and -2, and Treponema pallidum; We also investigated the potential usefulness of this M-PCR assay in screening programs for semen pathogens. In addition, we aimed: to detect human Papillomavirus (HPV and genotypes by single PCR (sPCR in the same semen samples; to determine the prevalence of the seven STDs, HPV and co-infections; to assess the possibility that these infections affect semen parameters and thus fertility. The overall validation parameters of M-PCR were extremely high including agreement (99.2%, sensitivity (100.00%, specificity (99.70%, positive (96.40% and negative predictive values (100.00% and accuracy (99.80%. The prevalence of STDs was very high (55.3%. Furthermore, associations were observed between STDs and changes in semen parameters, highlighting the importance of STD detection in semen. Thus, this M-PCR assay has great potential for application in semen screening programs for pathogens in infertility and STD clinics and in sperm banks.

  11. Impact of porcine circovirus type 2 (PCV2) vaccination on boar semen quality and quantity using two different vaccines

    NARCIS (Netherlands)

    Caspari, K; Henning, H; Schreiber, F; Maass, P; Gössl, R; Schaller, C; Waberski, D

    2014-01-01

    Porcine circovirus type-2 (PCV2) is widespread in domestic pig populations. It can be shed with boar semen, but the role boars have in epidemiology is still unclear. Vaccinating boars against PCV2 can reduce disease and virus load in semen, but may have unwanted side effects, that is, impairment of

  12. Cryopreservation of turkey semen: effects of breeding line and freezing method on post-thaw sperm quality, fertilization, and hatching

    NARCIS (Netherlands)

    Long, J.A.; Purdy, P.H.; Zuidberg, C.A.; Hiemstra, S.J.; Velleman, S.G.; Woelders, H.

    2014-01-01

    Cryopreservation methods for poultry semen are not reliable for germplasm preservation, especially for turkeys, where fertility rates from frozen/thawed semen are particularly low. The objective was to evaluate cryopreservation methods for effectiveness in promoting cryosurvival and post-thaw functi

  13. Intravaginal artificial insemination in bitches using frozen/thawed semen after dilution in powdered coconut water (ACP-106c).

    Science.gov (United States)

    Uchoa, D C; Silva, T F P; Mota Filho, A C; Silva, L D M

    2012-12-01

    The aim of this study was to evaluate powdered coconut water extender (ACP-106c; ACP Serviços Tecnológicos Ltda, ACP Biotecnologia, Fortaleza, Ceará, Brazil) as a diluent for freezing dog semen and the fertility after vaginal insemination of semen frozen therein. Ten ejaculates were collected from five dogs, evaluated fresh, diluted in ACP-106c, 10% egg yolk and 6% glycerol, cooled and frozen. In the first phase of the study, straws with frozen semen were thawed and immediately subjected to the same analysis as the fresh semen and, in addition, to Computer-Assisted Semen Analysis (CASA). In phase 2, 10 bitches that had been subjected to natural breeding during a preceding oestrous cycle were vaginally inseminated with thawed semen that had been re-diluted in ACP-106c. After thawing, a mean of 77% sperm motility was obtained through subjective analysis and 77.3% through CASA. Following artificial insemination, a 60% pregnancy rate was observed, resulting in a 50% parturition rate and a mean litter size of 3.4 (SEM 0.6), with 47.1% males and 52.9% females. ACP-106c can be successfully used for freezing canine semen, and vaginal deposition of such semen yields similar pregnancy rates to those reported in other studies.

  14. Selenium status and GSH-Px activity in semen and blood of boars at different ages used for artificial insemination.

    Science.gov (United States)

    Lasota, B; Błaszczyk, B; Seremak, B; Udała, J

    2004-10-01

    This study was performed to determine the relationship between selenium (Se) content and Se-dependent glutathione peroxidase (GSH-Px) activity in blood and semen, and semen quality of boars at different age used in an artificial insemination (AI) station. Routine macroscopic and microscopic analyses of semen quality were accompanied by measurement of Se content and GSH-Px activity in blood and semen. The Se concentration in blood plasma, seminal fluid and spermatozoa was measured by fluorometric method, the GSH-Px activity by a method based on NADPH-coupled reaction. A total of 155 ejaculates and 58 blood samples were investigated. The results of this study showed that there was no direct relationship between the Se content and GSH-Px activity in blood plasma and semen, and semen quality of sexual matured boars. The mechanisms controlling Se content and GSH-Px activity in blood and semen seem to be independent. The age of boars as a differentiating factor for Se content and GSH-Px activity in blood and semen is possible. It is concluded that a determination of Se status and/or GSH-Px activity in organism before Se supplementation is indicated.

  15. Does weight loss improve semen quality and reproductive hormones? Results from a cohort of severely obese men

    DEFF Research Database (Denmark)

    Håkonsen, Linn Berger; Thulstrup, Ane Marie; Aggerholm, Anette Skærbech;

    2011-01-01

    A high body mass index (BMI) has been associated with reduced semen quality and male subfecundity, but no studies following obese men losing weight have yet been published. We examined semen quality and reproductive hormones among morbidly obese men and studied if weight loss improved the reprodu...

  16. Sensitive simultaneous detection of seven sexually transmitted agents in semen by multiplex-PCR and of HPV by single PCR.

    Science.gov (United States)

    Gimenes, Fabrícia; Medina, Fabiana Soares; Abreu, André Luelsdorf Pimenta de; Irie, Mary Mayumi Taguti; Esquiçati, Isis Baroni; Malagutti, Natália; Vasconcellos, Vinícius Rodrigo Bulla; Discacciati, Michele Garcia; Bonini, Marcelo Gialluisi; Maria-Engler, Silvya Stuchi; Consolaro, Marcia Edilaine Lopes

    2014-01-01

    Sexually transmitted diseases (STDs) may impair sperm parameters and functions thereby promoting male infertility. To date limited molecular studies were conducted to evaluate the frequency and type of such infections in semen Thus, we aimed at conceiving and validating a multiplex PCR (M-PCR) assay for the simultaneous detection of the following STD pathogens in semen: Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, Herpes virus simplex (HSV) -1 and -2, and Treponema pallidum; We also investigated the potential usefulness of this M-PCR assay in screening programs for semen pathogens. In addition, we aimed: to detect human Papillomavirus (HPV) and genotypes by single PCR (sPCR) in the same semen samples; to determine the prevalence of the seven STDs, HPV and co-infections; to assess the possibility that these infections affect semen parameters and thus fertility. The overall validation parameters of M-PCR were extremely high including agreement (99.2%), sensitivity (100.00%), specificity (99.70%), positive (96.40%) and negative predictive values (100.00%) and accuracy (99.80%). The prevalence of STDs was very high (55.3%). Furthermore, associations were observed between STDs and changes in semen parameters, highlighting the importance of STD detection in semen. Thus, this M-PCR assay has great potential for application in semen screening programs for pathogens in infertility and STD clinics and in sperm banks.

  17. The effect of presence of facultative bacteria species on semen and sperm quality of men seeking fertility care

    Directory of Open Access Journals (Sweden)

    C.A. Enwuru

    2016-09-01

    Conclusions: Although bacterial presence in semen reduced mean sperm concentration and viability, thereby contributed to oligozoospermia and by extension the chances of siring a child, however, factors other than bacterial presence may contribute more. Improved interpretative approaches of semen analyses are highlighted.

  18. Resistencia bacteriana de cultivos de orina en un hospital oncológico:seguimiento a diez años

    Directory of Open Access Journals (Sweden)

    Consuelo Velázquez-Acosta

    2016-07-01

    Full Text Available Objetivo. Describir los patrones de resistencia bacteriana en cultivos de orina de pacientes de un hospital oncológico en la Ciudad de México, de 2004 a 2013. Material y métodos. Se obtuvo el porcentaje de susceptibilidad para diferentes antibióticos, describiendo por separado las bacterias multidrogorresistentes (MDR. Se analizaron por separado las cepas obtenidas de pacientes hospitalizados de las de la comunidad. Resultados. Se realizaron 51 202 cultivos, de los cuales se identificaron 14 480 bacterias (28.3%. De éstas, se reportaron 11 427 Gram negativos (78.9%; 2 080 Gram positivos (14.4%; y 973 (6.6% levaduras. Escherichia coli fue el principal microorganismo aislado (56.1%; 24% de las cepas de la comunidad y 66% de las nosocomiales fueron productoras de beta-lactamasas de espectro extendido (BLEE. Klebsiella pneumoniae se identificó en 705 cultivos (4.8%, 115 de los cuales fueron BLEE (16%: 13.1% de la comunidad y 29.8% nosocomiales. Pseudomonas aeruginosa se identificó en 593 cultivos (4.1%: 9% de la comunidad y 51% nosocomiales. Conclusiones. Las cepas MDR son mucho más frecuentes en muestras de origen nosocomial. Es prioritario intensificar el uso racional de antibióticos en la comunidad y el programa de desescalamiento de antimicrobianos en el hospital.   DOI: http://dx.doi.org/10.21149/spm.v58i4.8025

  19. Reunión regional de vigilancia de la resistencia bacteriana a los antibióticos 2009. Lima, Perú

    OpenAIRE

    Sacsaquispe Contreras, Rosa

    2010-01-01

    Artículo online La Reunión Regional de Vigilancia de la Resistencia Bacteriana a los Antibióticos 2009, tuvo como sede la ciudad de Lima, se realizó durante los días 03 y 04 de diciembre de 2009, y fue organizada por USAID y la Organización Panamericana de la Salud (OPS). A la actividad asistieron participantes de 20 países de la Región, representando a sus respectivos Ministerios de Salud, Institutos de Salud, OPS, además de centros como la Caribbean Epidemiology Center (CAREC).

  20. Amigdalitis aguda recurrente bacteriana: Estudio prospectivo, comparativo y controlado de sus características clínicas y microbiológicas

    OpenAIRE

    Der M,Carolina; Iñiguez C,Rodrigo; Guzman D,Ana Maria; Jofré P,David; Iñiguez C,Armando; Labarca L,Jaime

    2007-01-01

    Introducción: La amigdalitis aguda recurrente es una patología de frecuente consulta, es una de las indicaciones de amigdalectomía. No se sabe con exactitud el origen de esta patología. Objetivo: Identificar la microbiología y patrones de susceptibilidad de las bacterias en la amigdalitis aguda recurrente bacteriana (AARB) a los antimicrobianos más comúnmente en el medio nacional, usados en su tratamiento. Material y método: Se planificó un estudio prospectivo, controlado y ciego. Se evaluaro...

  1. Tireoidite bacteriana supurativa: relato de caso e revisão da literatura Acute suppurative thyroiditis: a case report and review of the literature

    Directory of Open Access Journals (Sweden)

    Adriana Vieira Pedreira

    2002-11-01

    Full Text Available Neste trabalho é apresentado um caso de tireoidite bacteriana aguda em um criança lúpica de nove anos de idade, em que o diagnóstico precoce foi imprescindível pela gravidade do quadro. Em seguida é apresentada uma revisão da literatura sobre o assunto.We present the case of a 9-year-old girl with acute suppurative thyroiditis and systemic lupus erythematosus. Early diagnosis was crucial for the outcome of the patient due to the severity of the disease. A review of the literature is also presented.

  2. Acumulación de antibióticos y su efecto sobre la comunidad bacteriana presentes en sedimentos de piscinas camaroneras

    OpenAIRE

    Uyaguari, Miguel; Montoya, Nelson

    2002-01-01

    Acumulación de antibióticos y su efecto sobre la comunidad bacteriana presentes en sedimentos de piscinas camaroneras La proliferación de enfermedades ha sido uno de los grandes retos con los que ha tenido que lidiar el sector camaronero. Las medidas profilácticas son lo más importante para prevenir el brote de enfermedades, sin embargo cuando el brote se manifiesta, la medicación es necesaria para evitar las altas mortalidades.

  3. Simulação, desenvolvimento de equipamento e validação experimental de ensaios não destrutivos com células bacterianas

    OpenAIRE

    Costa, Alexandre Manuel Afonso

    2014-01-01

    A técnica de Ensaios Não Destrutivos (END) baseada em células bacterianas (CB) foi proposta recentemente e tem demonstrado viabilidade na identificação de micro defeitos superficiais, com espessuras e profundidades inferiores a 5 μm, em vários materiais de engenharia. O conhecimento processual já é significativo, mas diversos aspectos carecem de desenvolvimentos, nomeadamente, a interacção das bactérias com outros materiais, o limiar de detectabilidade da técnica, a aplicação a defeitos na...

  4. Controle alternativo da murcha bacteriana do pimentão utilizando óleos essenciais vegetais e silicato de cálcio.

    OpenAIRE

    Aldenir de Oliveira Alves

    2012-01-01

    A murcha bacteriana do pimentão, causada por Ralstonia solanacearum (Rs) raça 1, é uma fitobacteriose importante em Pernambuco e outros estados do Brasil. Este trabalho teve como objetivos testar o efeito de óleos vegetais e do silício (Si) no controle alternativo desta doença, seus efeitos diretos sobre Rs e verificar os possíveis mecanismos envolvidos nestes processos. Os óleos essenciais de bergamota, canela, capim limão, copaíba, eucalipto citriodora, eucalipto globulus, funcho, hortel...

  5. Genética y genómica enfocadas en el estudio de la resistencia bacteriana Genetics and Genomics for the study of bacterial resistance

    OpenAIRE

    Ulises Garza-Ramos; Jesús Silva-Sánchez; Esperanza Martínez-Romero

    2009-01-01

    La resistencia bacteriana es un problema de salud pública causante de índices elevados de morbi-mortalidad hospitalaria. En la medida en que se usan los diferentes antibióticos se seleccionan bacterias resistentes a múltiples fármacos. El desarrollo de nuevas herramientas moleculares de la genómica y proteómica, como el PCR en tiempo real, pirosecuenciación de ADN, espectrometría de masas, microarreglos de ADN y bioinformática, permite conocer en forma más estrecha la fisiología y estructura ...

  6. Efecto de las condiciones de cultivo y purificación sobre las propiedades fisicoquímicas y de transporte en membranas de celulosa bacteriana

    OpenAIRE

    Carreño Pineda, Luz Dary

    2011-01-01

    En el presente trabajo fueron sintetizadas membranas de celulosa bacteriana a partir de un aislado nativo de Acetobacter xylinum. Se variaron como condiciones de cultivo la temperatura y la fuente de carbono, y como condiciones de purificación el agente purificante, la temperatura y el tiempo de exposición, y se evaluó el efecto de estas condiciones sobre las propiedades fisicoquímicas y de transporte de las membranas. Se caracterizaron con pruebas de difracción de rayos X, calorimetría di...

  7. Evaluación de la congelación para conservación de especies autóctonas bacterianas

    OpenAIRE

    2005-01-01

    Los microorganismos bacterianos constituyen una buena parte de la biodiversidad de nuestro planeta y la importancia de su conocimiento y preservación se ha incrementado a medida que se descubren sus múltiples utilidades en diferentes campos. La conservación se puede hacer por diferentes métodos y uno de los que brinda mejores resultados es la congelación; por ello se realizó este proyecto cuyo objetivo fue evaluar los efectos de la congelación en 18 especies bacterianas autóctonas de la colec...

  8. Molecular biology applied to the laboratory diagnosis of bacterial endophthalmitis Biologia molecular aplicada ao diagnóstico laboratorial de endoftalmite bacteriana

    Directory of Open Access Journals (Sweden)

    Paulo José Martins Bispo

    2009-10-01

    Full Text Available Bacterial endophthalmitis is a serious but uncommon intraocular infection which frequently results in vision loss. Early diagnosis and appropriate therapy are associated with better visual outcome. Conventional microbiological methods are currently used for microbiological characterization of eyes with suspected endophthalmitis. However, the sensitivity of bacterial detection from aqueous and vitreous humor using microbiology techniques is poor, and time-consuming to confirm the results. The application of molecular methods enhances significantly laboratory confirmation of bacterial endophthalmitis, demanding a shorter time to draw a definitive result and thereby promoting the early initiation of a more specific therapy to limit the empirical or unnecessary use of broad-spectrum antibiotics. PCR-based techniques, including post-PCR methods such RFLP, DNA probe hybridization and DNA sequencing have been successfully used for the diagnostic elucidation of clinically suspected bacterial endophthalmitis cases, showing promising application in the routine practice of ocular microbiology laboratories.Endoftalmite bacteriana é uma infecção intraocular grave, mas de baixa frequência, podendo resultar em grande prejuízo visual. O diagnóstico precoce e a rápida instituição de terapia adequada estão associadas a um melhor prognóstico da doença. Os métodos microbiológicos convencionais são utilizados rotineiramente para caracterização microbiológica de olhos com suspeita de endoftalmite. No entanto, a sensibilidade de detecção bacteriana em amostras de humor aquoso e vítreo utilizando técnicas microbiológicas é baixa, além de demandar um maior tempo para a confirmação dos resultados. A utilização de métodos moleculares aumenta significativamente os casos de endoftalmite bacteriana confirmados laboratorialmente, com tempo menor para a liberação de um resultado definitivo, auxiliando assim a instituição precoce de uma terapia

  9. Resina acrílica: relação entre tratamento superficial e retenção de placa bacteriana

    OpenAIRE

    Rego, Marina R. M.; Kitahara, Flávia M. F.; SANTIAGO,Luiz Carlos

    2010-01-01

    Este estudo avaliou a influência de técnicas de tratamento superficial da resina acrílica na adesão de placa bacteriana utilizando microbiota encontrado na cavidade bucal. Foram confeccionados noventa corpos de prova cilíndricos em resina acrílica autopolimerizável e divididos em nove grupos, para utilização de várias técnicas de tratamento superfi¬cial usualmente utilizadas nesse material. Os corpos-de-prova fora...

  10. Estratégias para prevenção da resistência bacteriana: contribuições para a segurança do paciente

    OpenAIRE

    Roberta Soldatelli Pagno Paim; Elisiane Lorenzini

    2014-01-01

    Introdução: A resistência bacteriana tem como consequências, o aumento da morbidade, mortalidade e da internação hospitalar, o prolongamento das doenças, a perda da produtividade, o aumento dos custos e maior risco de complicações. Materiais e Métodos: Trata-se de um estudo descritivo, com abordagem qualitativa, na perspectiva de uma revisão integrativa da literatura, realizada em janeiro de 2014. Resultados e Discussão: Foram selecionados oito artigos que relatavam estratégias de prevenção d...

  11. REMOÇÃO DE PLACA BACTERIANA DENTÁRIA APÓS O USO DE ENXAGUANTES BUCAIS PRÉ-ESCOVAÇÃO

    OpenAIRE

    Regina da Silva Miranda

    2012-01-01

    Este trabalho teve como objetivo avaliar a influência do uso de enxaguantes bucais pré-escovação na remoção da placa bacteriana por adolescentes. Foi realizado um estudo seguindo o modelo cruzado, de duplo mascaramento, com duração total de 25 dias, com 4 dias de intervalo entre as fases. O protocolo da pesquisa obteve aprovação pelo Comitê de Ética em pesquisa da UFSM. Trinta e oito adolescentes (idade média de 13 anos) de uma escola estadual da cidade de Santa MariaRS, uti...

  12. Estratégias para prevenção da resistência bacteriana: contribuições para a segurança do paciente

    Directory of Open Access Journals (Sweden)

    Roberta Soldatelli Pagno Paim

    2014-11-01

    Full Text Available Introdução: A resistência bacteriana tem como consequências, o aumento da morbidade, mortalidade e da internação hospitalar, o prolongamento das doenças, a perda da produtividade, o aumento dos custos e maior risco de complicações. Materiais e Métodos: Trata-se de um estudo descritivo, com abordagem qualitativa, na perspectiva de uma revisão integrativa da literatura, realizada em janeiro de 2014. Resultados e Discussão: Foram selecionados oito artigos que relatavam estratégias de prevenção da resistência bacteriana em um recorte temporal de 2008 a 2014. O uso racional de antimicrobianos, a higienização adequada das mãos, a cultura de vigilância microbiológica, a educação continuada, a desinfecção de superfícies, uso de testes de suscetibilidade, o isolamento de contato, quando indicado e a manutenção de um banco de dados, são alguns dos métodos encontrados neste trabalho para prevenir a seleção de micro-organismos resistentes. Conclusões: A diminuição do lançamento de novos antimicrobianos pela indústria farmacêutica e a necessidade de novos antibióticos reforça ainda mais a importância da conscientização de toda a equipe multidisciplinar no processo de prevenção de resistência antimicrobiana.            Palavras chave: Farmacorresistência Bacteriana, Antibacterianos, Programa de Controle de Infecção Hospitalar, Infecção Hospitalar, Segurança do Paciente. (Fonte: DeCS BIREME.Cómo citar este artículo: Paim R, Lorenzini E. Estrategias para prevenção da resistência bacteriana. Rev Cuid. 2014; 5(2:757-64. http://dx.doi.org/10.15649/cuidarte.v5i2.88

  13. Desarrollo de consorcios de diazótrofos con actividad promotora de crecimiento en plantas de arroz inundado y secano en los departamentos del Tolima y Meta

    OpenAIRE

    2012-01-01

    La actividad de rizobacterias promotoras de crecimiento vegetal (PGPR) se ha potenciado mediante mezclas bacterianas. Sin embargo, no todas las mezclas son exitosas por lo que la selección de cepas es un paso crítico para la conformación de tales inóculos. En este trabajo se realizó una caracterización agroecológica de bacterias fijadoras de N2 en 16 fincas arroceras de los departamentos del Tolima y Meta. Las zonas del Tolima y Meta fueron diferenciadas por las características físico-química...

  14. INFECCION VAGINAL Y TRATAMIENTO DEL STREPTOCOCCUS GRUPO B EN EMBARAZADAS CON FACTORES UNIVERSALES DE RIESGO DE INFECCION: RESULTADOS NEONATALES Y FACTORES DE RIESGO DE INFECCION NEONATAL

    OpenAIRE

    Ovalle S,Alfredo; Gómez M,Ricardo; Martínez T,M Angélica; Aspillaga M,Carlos; Dolz C,Sergio

    2002-01-01

    Objetivos: Determinar la eficacia del tratamiento antibiótico en los resultados neonatales en embarazadas con factores universales de riesgo de infección e infección vaginal por Streptococcus grupo B (SGB) y conocer los factores de riesgo para infección neonatal por esta bacteria. Diseño del estudio: Las mujeres con infección vaginal por SGB en una población de cuatrocientos cuarenta y tres embarazadas con riesgo universal de infección bacteriana fueron incorporadas en este estudio. Se consid...

  15. Análise microbiológica da placa bacteriana da doença periodontal em cães e o efeito da antibioticoterapia sobre ela

    OpenAIRE

    Fonseca, Stella Alves da; Galera,Paula Diniz; Brito, Daiana Lima; Perecmanis,Simone; Silva,Anahí Souza; Cardoso, Larissa Borges; Marçola,Tatiana Guerrero; Drummond,Vinícius Oliveira; Pimentel,Concepta McManus

    2011-01-01

    Objetivou-se determinar a microbiota da placa bacteriana subgengival de cães com doença periodontal (DP) e estabelecer o efeito da antibioticoterapia. Avaliaram-se 20 cães com graus variados de DP e coletaram-se amostras da placa bacteriana subgengival antes e após antibioticoterapia. Preconizou-se antibioticoterapia distinta em dois grupos, com 10 animais cada: clindamicina (G1) e metronidazol + espiramicina (G2). Observou-se crescimento bacteriano subgengival na maioria dos cães com DP e co...

  16. Lead exposure and semen quality among traffic police in Arequipa, Peru.

    Science.gov (United States)

    Eibensteiner, Lynn; Del Carpio Sanz, Ada; Frumkin, Howard; Gonzales, Carla; Gonzales, Gustavo F

    2005-01-01

    This study examined lead exposure (n = 43) and semen quality (n = 18) among traffic police officers in Arequipa, Peru, where leaded gasoline is used. Blood lead (PbB) was measured using atomic absorption spectrophotometry, and semen was analyzed following World Health Organization (WHO) protocol. Mean PbB was 48.5 microg/dL. Although current PbB was associated with declines in several semen parameters (sperm morphology, concentration and total number of sperm), only sperm motility and viability differed significantly between the 40 microg/dL categories, and decreased with increasing PbB in simple linear regression. Traffic police are an indicator group for excessive ambient lead exposure, and these results support earlier findings on the male reproductive toxicity of lead. The results should be interpreted cautiously since the numbers were small and the analysis was unable to control for all potential confounders due to incomplete data.

  17. SPERM MORPHOLOGICAL ASSESSMENTS OF FRIESIAN HOLSTEIN BULL SEMEN COLLECTED FROM THREE ARTIFICIAL INSEMINATION CENTERS IN INDONESIA

    Directory of Open Access Journals (Sweden)

    B. Purwantara

    2014-10-01

    Full Text Available Morphologically abnormal sperm in semen has been associated with the sub fertility and sterilityfor many years. This study assessed the sperm morphology of Friesian Holstein bull semen which wascollected from three Artificial Insemination centers in Indonesia. Total of 22 bulls were used in thisstudy; an ejaculate from each bull was examined. Three to four glass slides were prepared for each bullsample; a drop of semen was placed on each glass slide, smeared, and air-dried. The smeared sampleswere stained with carbolfuchsin-eosin (Williams stain. Morphological abnormality types were recordedfrom total of 500 spermatozoa. Results demonstrated that all 22 bulls had low sperm abnormality(<10%. Pearshaped was the most frequently type of sperm abnormality found in the samples(0.81±0.93%; while detached head was the lowest (0.01±0.04%.

  18. The Canonical Correlation Analysis on Semen Quality and Serum Heavy Metals in Chinese Young Men

    Institute of Scientific and Technical Information of China (English)

    Jun-qing WU; Jiang ZHU; Zhan-hai FU; Yin-mei DU; Cui-ling LIANG; Er-sheng GAO; Jian-guo TAO; Qiu-ying YANG; Xiao XU; Wen-juan CAI; Jian GUO; Feng TANG

    2003-01-01

    Objective To explore the correlation between serum heavy metal and semen quality in normal Chinese young menMethods This study was designed as a multi-center cross-sectional investigation. The subjects consisted of 562 male vomunteers who had undergone premarital physical examination in maternal and children health centers in 7 provinces in China.Results Results from Spearman rank correlation analysis (partial variable: region) show that serum lead and cadmium are negatively related to percentage of morphological normal sperm, but canonical correlation between semen quality and serum heavy metal are not significant. Canonical correlation analysis among the subjects from Guizhou shows cadmium is harmful to sperm morphology. In Henan, furthermore, results show lead and cadmium could negatively affect sperm viability and morphology.Conclusion Among all study subjects, canonical correlation between semen quality and serum heavy metal were not significant; however, results in some region showed serum cadmium and lead might be harmful to sperm quality.

  19. Effect of cholesterol-loaded-cyclodextrin on sperm viability and acrosome reaction in boar semen cryopreservation.

    Science.gov (United States)

    Lee, Yong-Seung; Lee, Seunghyung; Lee, Sang-Hee; Yang, Boo-Keun; Park, Choon-Keun

    2015-08-01

    This study was undertaken to examine the effect of cholesterol-loaded-cyclodextrin (CLC) on boar sperm viability and spermatozoa cryosurvival during boar semen cryopreservation, and methyl-β-cyclodextrin (MBCD) was treated for comparing with CLC. Boar semen treated with CLC and MBCD before freezing process to monitor the effect on survival and capacitation status by flow cytometry with appropriate fluorescent probes. Sperm viability was higher in 1.5mg CLC-treated sperm (76.9±1.01%, Psperm before cryopreservation (58.7±1.31% and 60.3±0.31%, respectively). For CTC patterns, F-pattern was higher in CLC treated sperm than MBCD-treated sperm, for B-pattern was higher in CLC-treated sperm than fresh sperm (Psperm) was lower in CLC-treated sperm than MBCD-treated sperm (Pboar semen.

  20. Human semen quality in relation to dietary pesticide exposure and organic diet

    DEFF Research Database (Denmark)

    Juhler, R. K.; Larsen, S. B.; Meyer, Otto A.

    1999-01-01

    The objective of the study was to corroborate or refute the hypothesis that farmers having a high intake of organic grown commodities have a high semen quality due to their expected lower level of dietary pesticides intake. Food frequency data and semen were collected from 256 farmers (171...... traditional farmers and 85 organic farmers, overall participation rate: 32%) who were selected from central registers. Each farmer delivered one semen sample before the spraying season started. The farmers were divided into three groups where the commodities from organic production contributed no (N, 0......%), medium (M, 1-49%), or a high (H, 50-100%) proportion of the fruit and vegetables consumed. Farmers having a high relative intake of organically grown fruit and vegetables also had a high relative consumption of organically produced meat, milk, and bread, and differences were observed comparing the actual...