Sample records for bacterial triphosphate tunnel

  1. Bacterial adenosine triphosphate as a measure of urinary tract infection (United States)

    Chappelle, E. W.; Picciolo, G. L.


    Procedure detects and counts bacteria present in urine samples. Method also determines bacterial levels in other aqueous body fluids including lymph fluid, plasma, blood, spinal fluid, saliva and mucous.

  2. Monitoring of bacterial contamination of dental unit water lines using adenosine triphosphate bioluminescence. (United States)

    Watanabe, A; Tamaki, N; Yokota, K; Matsuyama, M; Kokeguchi, S


    Bacterial contamination of dental unit waterlines (DUWLs) was evaluated using ATP bioluminescence analysis and a conventional culture method. Water samples (N=44) from DUWLs were investigated for heterotrophic bacteria by culture on R2A agar, which gave counts ranging from 1.4×10(3) to 2.7×10(5) cfu/mL. The ATP bioluminescence results for DUWL samples ranged from 6 to 1189 relative light units and could be obtained within 1min; these correlated well with the culture results (r=0.727-0.855). We conclude that the results of the ATP bioluminescence assay accurately reflect the results of conventional culture-based testing. This method is potentially useful for rapid and simple monitoring of DUWL bacterial contamination.

  3. Imaging Adenosine Triphosphate (ATP). (United States)

    Rajendran, Megha; Dane, Eric; Conley, Jason; Tantama, Mathew


    Adenosine triphosphate (ATP) is a universal mediator of metabolism and signaling across unicellular and multicellular species. There is a fundamental interdependence between the dynamics of ATP and the physiology that occurs inside and outside the cell. Characterizing and understanding ATP dynamics provide valuable mechanistic insight into processes that range from neurotransmission to the chemotaxis of immune cells. Therefore, we require the methodology to interrogate both temporal and spatial components of ATP dynamics from the subcellular to the organismal levels in live specimens. Over the last several decades, a number of molecular probes that are specific to ATP have been developed. These probes have been combined with imaging approaches, particularly optical microscopy, to enable qualitative and quantitative detection of this critical molecule. In this review, we survey current examples of technologies available for visualizing ATP in living cells, and identify areas where new tools and approaches are needed to expand our capabilities.

  4. Behavior and stability of adenosine triphosphate (ATP) during chlorine disinfection. (United States)

    Nescerecka, Alina; Juhna, Talis; Hammes, Frederik


    Adenosine triphosphate (ATP) analysis is a cultivation-independent alternative method for the determination of bacterial viability in both chlorinated and non-chlorinated water. Here we investigated the behavior and stability of ATP during chlorination in detail. Different sodium hypochlorite doses (0-22.4 mg-Cl2 L(-1); 5 min exposure) were applied to an Escherichia coli pure culture suspended in filtered river water. We observed decreasing intracellular ATP with increasing chlorine concentrations, but extracellular ATP concentrations only increased when the chlorine dose exceeded 0.35 mg L(-1). The release of ATP from chlorine-damaged bacteria coincided with severe membrane damage detected with flow cytometry (FCM). The stability of extracellular ATP was subsequently studied in different water matrixes, and we found that extracellular ATP was stable in sterile deionized water and also in chlorinated water until extremely high chlorine doses (≤11.2 mg-Cl2 L(-1); 5 min exposure). In contrast, ATP decreased relatively slowly (k = 0.145 h(-1)) in 0.1 μm filtered river water, presumably due to degradation by either extracellular enzymes or the fraction of bacteria that were able to pass through the filter. Extracellular ATP decreased considerably faster (k = 0.368 h(-1)) during batch growth of a river water bacterial community. A series of growth potential tests showed that extracellular ATP molecules were utilized as a phosphorus source during bacteria proliferation. From the combined data we conclude that ATP released from bacteria at high chlorine doses could promote bacteria regrowth, contributing to biological instability in drinking water distribution systems.

  5. Thiamine diphosphate adenylyl transferase from E. coli: functional characterization of the enzyme synthesizing adenosine thiamine triphosphate


    Brans Alain; Makarchikov Alexander F; Bettendorff Lucien


    Abstract Background We have recently identified a new thiamine derivative, adenosine thiamine triphosphate (AThTP), in E. coli. In intact bacteria, this nucleotide is synthesized only in the absence of a metabolizable carbon source and quickly disappears as soon as the cells receive a carbon source such as glucose. Thus, we hypothesized that AThTP may be a signal produced in response to carbon starvation. Results Here we show that, in bacterial extracts, the biosynthesis of AThTP is carried o...

  6. Short tunnels.

    NARCIS (Netherlands)

    Schreuder, D.A.


    Before dealing with the question of lighting short tunnels, it is necessary define what is meant by a tunnel and when it should be called 'short'. Confined to motorized road traffic the following is the most apt definition of a tunnel: every form of roofing-over a road section, irrespective of it le

  7. Nucleoside triphosphate synthesis catalysed by adenylate kinase is ADP dependent

    DEFF Research Database (Denmark)

    Willemoës, Martin; Kilstrup, Mogens


    Adenylate kinase (Adk) that catalyses the synthesis of ADP from ATP and AMP has also been shown to perform an ATP dependent phosphorylation of ribo- and deoxynucleoside diphosphates to their corresponding nucleoside triphosphate; ATP+(d)NDPADP+(d)NTP. This reaction, suggested to occur by the tran......Adenylate kinase (Adk) that catalyses the synthesis of ADP from ATP and AMP has also been shown to perform an ATP dependent phosphorylation of ribo- and deoxynucleoside diphosphates to their corresponding nucleoside triphosphate; ATP+(d)NDPADP+(d)NTP. This reaction, suggested to occur...

  8. 21 CFR 864.7040 - Adenosine triphosphate release assay. (United States)


    ... device that measures the release of adenosine triphosphate (ATP) from platelets following aggregation. This measurement is made on platelet-rich plasma using a photometer and a luminescent firefly extract. Simultaneous measurements of platelet aggregation and ATP release are used to evaluate platelet...

  9. Nucleoside triphosphate synthesis catalysed by adenylate kinase is ADP dependent

    DEFF Research Database (Denmark)

    Willemoes, Martin; Kilstrup, M.


    Adenylate kinase (Adk) that catalyses the synthesis of ADP from ATP and AMP has also been shown to perform an ATP dependent phosphorylation of ribo- and deoxynucleoside diphosphates to their corresponding nucleoside triphosphate; ATP + (d)NDP ¿ ADP + (d)NTP. This reaction, suggested to occur...

  10. Modified Nucleoside Triphosphates for in-vitro Selection Techniques (United States)

    Iribarren, Adolfo; Dellafiore, María; Montserrat, Javier


    The development of SELEX (Selective Enhancement of Ligands by Exponential Enrichment) provides a powerful tool for the search of functional oligonucleotides with the ability to bind ligands with high affinity and selectivity (aptamers) and for the discovery of nucleic acid sequences with diverse enzymatic activities (ribozymes and DNAzymes). This technique has been extensively applied to the selection of natural DNA or RNA molecules but, in order to improve chemical and structural diversity as well as for particular applications where further chemical or biological stability is necessary, the extension of this strategy to modified oligonucleotides is desirable. Taking into account these needs, this review intends to collect the research carried out during the past years, focusing mainly on the use of modified nucleotides in SELEX and the development of mutant enzymes for broadening nucleoside triphosphates acceptance. In addition, comments regarding the synthesis of modified nucleoside triphosphate will be briefly discussed.

  11. Synthesis of (gamma-/sup 32/P)thiamine triphosphate

    Energy Technology Data Exchange (ETDEWEB)

    Grandfils, C.; Bettendorff, L.; de Rycker, C.; Schoffeniels, E.


    We developed a novel chemical synthesis of thiamine triphosphate which allows us to incorporate /sup 32/P in the gamma position. The reaction is based on the condensation of (/sup 32/P)orthophosphoric acid and thiamine diphosphate in the presence of ethyl chloroformate. After purification by two ion-exchange purification steps, the thiamine derivative has a specific radioactivity of 10 Ci/mmol. The average final yield synthesis is about 10%.

  12. Adenosine triphosphate (ATP) as a possible indicator of extraterrestrial biology (United States)

    Chappelle, E. W.; Picciolo, G. L.


    The ubiquity of adenosine triphosphate (ATP) in terrestrial organisms provides the basis for proposing the assay of this vital metabolic intermediate for detecting extraterrestrial biological activity. If an organic carbon chemistry is present on the planets, the occurrence of ATP is possible either from biosynthetic or purely chemical reactions. However, ATP's relative complexity minimizes the probability of abiogenic synthesis. A sensitive technique for the quantitative detection of ATP was developed using the firefly bioluminescent reaction. The procedure was used successfully for the determination of the ATP content of soil and bacteria. This technique is also being investigated from the standpoint of its application in clinical medicine.

  13. Treatment of paroxysmal supraventricular tachycardia with intravenous injection of adenosine triphosphate.


    Saito, D.; Ueeda, M; Abe, Y.; Tani, H; Nakatsu, T.; Yoshida, H.; Haraoka, S; Nagashima, H


    Intravenous adenosine triphosphate rapidly terminated all 11 episodes of paroxysmal supraventricular tachycardia in 10 patients. Eight patients reported side effects but these resolved within 20 seconds and did not require treatment. Adenosine triphosphate is a suitable agent for the rapid termination of paroxysmal supraventricular tachycardia.

  14. Autophagy occurs within an hour of adenosine triphosphate treatment after nerve cell damage:the neuroprotective effects of adenosine triphosphate against apoptosis

    Institute of Scientific and Technical Information of China (English)

    Na Lu; Baoying Wang; Xiaohui Deng; Honggang Zhao; Yong Wang; Dongliang Li


    After hypoxia, ischemia, or inlfammatory injuries to the central nervous system, the damaged cells release a large amount of adenosine triphosphate, which may cause secondary neuronal death. Autophagy is a form of cell death that also has neuroprotective effects. Cell Counting Kit assay, monodansylcadaverine staining, lfow cytometry, western blotting, and real-time PCR were used to determine the effects of exogenous adenosine triphosphate treatment at different concentrations (2, 4, 6, 8, 10 mmol/L) over time (1, 2, 3, and 6 hours) on the apoptosis and autophagy of SH-SY5Y cells. High concentrations of extracellular adenosine triphosphate induced autophagy and apoptosis of SH-SY5Y cells. The enhanced autophagy ifrst appeared, and peaked at 1 hour after treatment with adenosine triphosphate. Cell apoptosis peaked at 3 hours, and persisted through 6 hours. With prolonged exposure to the adenosine triphosphate treatment, the fraction of apoptotic cells increased. These data suggest that the SH-SY5Y neural cells initiated autophagy against apoptosis within an hour of adenosine triphosphate treatment to protect themselves against injury.

  15. Adenylate kinase-independent thiamine triphosphate accumulation under severe energy stress in Escherichia coli

    Directory of Open Access Journals (Sweden)

    Wins Pierre


    Full Text Available Abstract Background Thiamine triphosphate (ThTP exists in most organisms and might play a role in cellular stress responses. In E. coli, ThTP is accumulated in response to amino acid starvation but the mechanism of its synthesis is still a matter of controversy. It has been suggested that ThTP is synthesized by an ATP-dependent specific thiamine diphosphate kinase. However, it is also known that vertebrate adenylate kinase 1 catalyzes ThTP synthesis at a very low rate and it has been postulated that this enzyme is responsible for ThTP synthesis in vivo. Results Here we show that bacterial, as vertebrate adenylate kinases are able to catalyze ThTP synthesis, but at a rate more than 106-fold lower than ATP synthesis. This activity is too low to explain the high rate of ThTP accumulation observed in E. coli during amino acid starvation. Moreover, bacteria from the heat-sensitive CV2 strain accumulate high amounts of ThTP (>50% of total thiamine at 37°C despite complete inactivation of adenylate kinase and a subsequent drop in cellular ATP. Conclusion These results clearly demonstrate that adenylate kinase is not responsible for ThTP synthesis in vivo. Furthermore, they show that E. coli accumulate large amounts of ThTP under severe energy stress when ATP levels are very low, an observation not in favor of an ATP-dependent mechanisms for ThTP synthesis.

  16. 2-Selenouridine triphosphate synthesis and Se-RNA transcription. (United States)

    Sun, Huiyan; Jiang, Sibo; Caton-Williams, Julianne; Liu, Hehua; Huang, Zhen


    2-Selenouridine ((Se)U) is one of the naturally occurring modifications of Se-tRNAs ((Se)U-RNA) at the wobble position of the anticodon loop. Its role in the RNA-RNA interaction, especially during the mRNA decoding, is elusive. To assist the research exploration, herein we report the enzymatic synthesis of the (Se)U-RNA via 2-selenouridine triphosphate ((Se)UTP) synthesis and RNA transcription. Moreover, we have demonstrated that the synthesized (Se)UTP is stable and recognizable by T7 RNA polymerase. Under the optimized conditions, the transcription yield of (Se)U-RNA can reach up to 85% of the corresponding native RNA. Furthermore, the transcribed (Se)U-hammerhead ribozyme has the similar activity as the corresponding native, which suggests usefulness of (Se)U-RNAs in function and structure studies of noncoding RNAs, including the Se-tRNAs.

  17. Inositol tri-phosphate inhuman and ascidian spermatozoa. (United States)

    Tosti, E; Palumbo, A; Dale, B


    Using a specific protein binding assay we have shown that a spermatozoon of the ascidian Ciona intestinalis contains 1.58 +/- 0.74 x 10(-19) moles of inositol 1,4,5-tri-phosphate (InsP3), while a human spermatozoon contains 6.4 +/- 0.14 x 10(-19) moles. Induction of the acrosome reaction (AR) in both species, by exposure to the calcium ionophore A23187, does not significantly alter levels of InsP3, suggesting that phosphatidylinositol (PI) turnover is not necessary for the calcium ionophore induced AR. Furthermore, PI turnover in ascidian spermatozoa appears to be insensitive to lithium and phorbol ester. The high intracellular concentration of InsP3 in spermatozoa, corresponding to 50-200 microM, suggests it may play a role in egg activation.

  18. Crystal Structure of a Legionella pneumophila Ecto -Triphosphate Diphosphohydrolase, A Structural and Functional Homolog of the Eukaryotic NTPDases

    Energy Technology Data Exchange (ETDEWEB)

    Vivian, Julian P.; Riedmaier, Patrice; Ge, Honghua; Le Nours, Jérôme; Sansom, Fiona M.; Wilce, Matthew C.J.; Byres, Emma; Dias, Manisha; Schmidberger, Jason W.; Cowan, Peter J.; d' Apice, Anthony J.F.; Hartland, Elizabeth L.; Rossjohn, Jamie; Beddoe, Travis (Monash); (Melbourne)


    Many pathogenic bacteria have sophisticated mechanisms to interfere with the mammalian immune response. These include the disruption of host extracellular ATP levels that, in humans, is tightly regulated by the nucleoside triphosphate diphosphohydrolase family (NTPDases). NTPDases are found almost exclusively in eukaryotes, the notable exception being their presence in some pathogenic prokaryotes. To address the function of bacterial NTPDases, we describe the structures of an NTPDase from the pathogen Legionella pneumophila (Lpg1905/Lp1NTPDase) in its apo state and in complex with the ATP analog AMPPNP and the subtype-specific NTPDase inhibitor ARL 67156. Lp1NTPDase is structurally and catalytically related to eukaryotic NTPDases and the structure provides a basis for NTPDase-specific inhibition. Furthermore, we demonstrate that the activity of Lp1NTPDase correlates directly with intracellular replication of Legionella within macrophages. Collectively, these findings provide insight into the mechanism of this enzyme and highlight its role in host-pathogen interactions.

  19. Detection of somatic coliphages through a bioluminescence assay measuring phage mediated release of adenylate kinase and adenosine 5'-triphosphate. (United States)

    Guzmán Luna, Carolina; Costán-Longares, Ana; Lucena, Francisco; Jofre, Joan


    The feasibility of detecting somatic coliphages by phage infection of Escherichia coli WG5 and measurement of phage propagation by the lysis mediated release of the bacterial host adenylate kinase (AK) and adenosine 5'-triphosphate (ATP) detected by a bioluminescent signal was evaluated. After 2h of incubation, all cultures infected with reference bacteriophage phiX174 showed a significant increase in the bioluminescent signal, even with number of phages as low as less of 10 plaque forming units (PFU). Naturally occurring somatic coliphages ensured a significant bioluminescent signal after 3h of infection when >10 PFU were inoculated. These results indicate that an easy and reliable method to detect low numbers of coliphages in less than 3h is feasible.

  20. Adenosine thiamine triphosphate accumulates in Escherichia coli cells in response to specific conditions of metabolic stress

    Directory of Open Access Journals (Sweden)

    Zorzi Willy


    Full Text Available Abstract Background E. coli cells are rich in thiamine, most of it in the form of the cofactor thiamine diphosphate (ThDP. Free ThDP is the precursor for two triphosphorylated derivatives, thiamine triphosphate (ThTP and the newly discovered adenosine thiamine triphosphate (AThTP. While, ThTP accumulation requires oxidation of a carbon source, AThTP slowly accumulates in response to carbon starvation, reaching ~15% of total thiamine. Here, we address the question whether AThTP accumulation in E. coli is triggered by the absence of a carbon source in the medium, the resulting drop in energy charge or other forms of metabolic stress. Results In minimal M9 medium, E. coli cells produce AThTP not only when energy substrates are lacking but also when their metabolization is inhibited. Thus AThTP accumulates in the presence of glucose, when glycolysis is blocked by iodoacetate, or in the presence lactate, when respiration is blocked by cyanide or anoxia. In both cases, ATP synthesis is impaired, but AThTP accumulation does not appear to be a direct consequence of reduced ATP levels. Indeed, in the CV2 E. coli strain (containing a thermolabile adenylate kinase, the ATP content is very low at 37°C, even in the presence of metabolizable substrates (glucose or lactate and under these conditions, the cells produce ThTP but not AThTP. Furthermore, we show that ThTP inhibits AThTP accumulation. Therefore, we conclude that a low energy charge is not sufficient to trigger AThTP accumulation and the latter can only accumulate under conditions where no ThTP is synthesized. We further show that AThTP production can also be induced by the uncoupler CCCP but, unexpectedly, this requires the presence of pyruvate or a substrate yielding pyruvate (such a D-glucose or L-lactate. Under the conditions described, AThTP production is not different when RelA or SpoT mutants are used. Conclusions In E. coli, AThTP accumulates in response to two different conditions of

  1. Carpal Tunnel Syndrome (United States)

    ... arm. Just a passing cramp? It could be carpal tunnel syndrome. The carpal tunnel is a narrow passageway of ligament and ... difficult. Often, the cause is having a smaller carpal tunnel than other people do. Other causes include ...

  2. Intracellular Adenosine Triphosphate Deprivation through Lanthanide-Doped Nanoparticles. (United States)

    Tian, Jing; Zeng, Xiao; Xie, Xiaoji; Han, Sanyang; Liew, Oi-Wah; Chen, Yei-Tsung; Wang, Lianhui; Liu, Xiaogang


    Growing interest in lanthanide-doped nanoparticles for biological and medical uses has brought particular attention to their safety concerns. However, the intrinsic toxicity of this new class of optical nanomaterials in biological systems has not been fully evaluated. In this work, we systematically evaluate the long-term cytotoxicity of lanthanide-doped nanoparticles (NaGdF4 and NaYF4) to HeLa cells by monitoring cell viability (mitochondrial activity), adenosine triphosphate (ATP) level, and cell membrane integrity (lactate dehydrogenase release), respectively. Importantly, we find that ligand-free lanthanide-doped nanoparticles induce intracellular ATP deprivation of HeLa cells, resulting in a significant decrease in cell viability after exposure for 7 days. We attribute the particle-induced cell death to two distinct cell death pathways, autophagy and apoptosis, which are primarily mediated via the interaction between the nanoparticle and the phosphate group of cellular ATP. The understanding gained from the investigation of cytotoxicity associated with lanthanide-doped nanoparticles provides keen insights into the safe use of these nanoparticles in biological systems.

  3. Adenylate kinase 1 knockout mice have normal thiamine triphosphate levels. (United States)

    Makarchikov, Alexander F; Wins, Pierre; Janssen, Edwin; Wieringa, Bé; Grisar, Thierry; Bettendorff, Lucien


    Thiamine triphosphate (ThTP) is found at low concentrations in most animal tissues and it may act as a phosphate donor for the phosphorylation of proteins, suggesting a potential role in cell signaling. Two mechanisms have been proposed for the enzymatic synthesis of ThTP. A thiamine diphosphate (ThDP) kinase (ThDP+ATP if ThTP+ADP) has been purified from brewer's yeast and shown to exist in rat liver. However, other data suggest that, at least in skeletal muscle, adenylate kinase 1 (AK1) is responsible for ThTP synthesis. In this study, we show that AK1 knockout mice have normal ThTP levels in skeletal muscle, heart, brain, liver and kidney, demonstrating that AK1 is not responsible for ThTP synthesis in those tissues. We predict that the high ThTP content of particular tissues like the Electrophorus electricus electric organ, or pig and chicken skeletal muscle is more tightly correlated with high ThDP kinase activity or low soluble ThTPase activity than with non-stringent substrate specificity and high activity of adenylate kinase.

  4. Kinetic and biochemical characterization of Trypanosoma evansi nucleoside triphosphate diphosphohydrolase. (United States)

    Weiss, Paulo Henrique Exterchoter; Batista, Franciane; Wagner, Glauber; Magalhães, Maria de Lourdes Borba; Miletti, Luiz Claudio


    Nucleoside triphosphate diphospho-hydrolases (NTPDases) catalyze the hydrolysis of several nucleosides tri and diphosphate playing major roles in eukaryotes including purinergic signaling, inflammation, hemostasis, purine salvage and host-pathogen interactions. These enzymes have been recently described in parasites where several evidences indicated their involvement in virulence and infection. Here, we have investigated the presence of NTPDase in the genome of Trypanosoma evansi. Based on the genomic sequence from Trypanosoma brucei, we have amplified an 1812 gene fragment corresponding to the T. evansi NTPDase gene. The protein was expressed in the soluble form and purified to homogeneity and enzymatic assays were performed confirming the enzyme identity. Kinetic parameters and substrate specificity were determined. The dependence of cations on enzymatic activity was investigated indicating the enzyme is stimulated by divalent cations and carbohydrates but inhibited by sodium. Bioinformatic analysis indicates the enzyme is a membrane bound protein facing the extracellular side of the cell with 98% identity to the T. brucei homologous NTPDase gene.

  5. Electron tunnel sensor technology (United States)

    Waltman, S. B.; Kaiser, W. J.


    The recent development of Scanning Tunneling Microscopy technology allows the application of electron tunneling to position detectors for the first time. The vacuum tunnel junction is one of the most sensitive position detection mechanisms available. It is also compact, simple, and requires little power. A prototype accelerometer based on electron tunneling, and other sensor applications of this promising new technology are described.

  6. The synthesis of 2′-methylseleno adenosine and guanosine 5′-triphosphates


    Santner, Tobias; Siegmund, Vanessa; Marx, Andreas; Micura, Ronald


    Modified nucleoside triphosphates (NTPs) represent powerful building blocks to generate nucleic acids with novel properties by enzymatic synthesis. We have recently demonstrated the access to 20-SeCH3-uridine and 20-SeCH3-cytidine derivatized RNAs for applications in RNA crystallography, using the correspondingnucleoside triphosphates and distinct mutants of T7 RNA polymerase. In the present note, we introduce the chemical synthesis of the novel 20-methylseleno-20-deoxyadenosine and -guanosin...

  7. Adenosine triphosphate inhibits melatonin synthesis in the rat pineal gland. (United States)

    Souza-Teodoro, Luis Henrique; Dargenio-Garcia, Letícia; Petrilli-Lapa, Camila Lopes; Souza, Ewerton da Silva; Fernandes, Pedro A C M; Markus, Regina P; Ferreira, Zulma S


    Adenosine triphosphate (ATP) is released onto the pinealocyte, along with noradrenaline, from sympathetic neurons and triggers P2Y1 receptors that enhance β-adrenergic-induced N-acetylserotonin (NAS) synthesis. Nevertheless, the biotransformation of NAS into melatonin, which occurs due to the subsequent methylation by acetylserotonin O-methyltransferase (ASMT; EC, has not yet been evaluated in the presence of purinergic stimulation. We therefore evaluated the effects of purinergic signaling on melatonin synthesis induced by β-adrenergic stimulation. ATP increased NAS levels, but, surprisingly, inhibited melatonin synthesis in an inverse, concentration-dependent manner. Our results demonstrate that enhanced NAS levels, which depend on phospholipase C (PLC) activity (but not the induction of gene transcription), are a post-translational effect. By contrast, melatonin reduction is related to an ASMT inhibition of expression at both the gene transcription and protein levels. These results were independent of nuclear factor-kappa B (NF-kB) translocation. Neither the P2Y1 receptor activation nor the PLC-mediated pathway was involved in the decrease in melatonin, indicating that ATP regulates pineal metabolism through different mechanisms. Taken together, our data demonstrate that purinergic signaling differentially modulates NAS and melatonin synthesis and point to a regulatory role for ATP as a cotransmitter in the control of ASMT, the rate-limiting enzyme in melatonin synthesis. The endogenous production of melatonin regulates defense responses; therefore, understanding the mechanisms involving ASMT regulation might provide novel insights into the development and progression of neurological disorders since melatonin presents anti-inflammatory, neuroprotective, and neurogenic effects.

  8. Thiamine diphosphate adenylyl transferase from E. coli: functional characterization of the enzyme synthesizing adenosine thiamine triphosphate

    Directory of Open Access Journals (Sweden)

    Brans Alain


    Full Text Available Abstract Background We have recently identified a new thiamine derivative, adenosine thiamine triphosphate (AThTP, in E. coli. In intact bacteria, this nucleotide is synthesized only in the absence of a metabolizable carbon source and quickly disappears as soon as the cells receive a carbon source such as glucose. Thus, we hypothesized that AThTP may be a signal produced in response to carbon starvation. Results Here we show that, in bacterial extracts, the biosynthesis of AThTP is carried out from thiamine diphosphate (ThDP and ADP or ATP by a soluble high molecular mass nucleotidyl transferase. We partially purified this enzyme and characterized some of its functional properties. The enzyme activity had an absolute requirement for divalent metal ions, such as Mn2+ or Mg2+, as well as for a heat-stable soluble activator present in bacterial extracts. The enzyme has a pH optimum of 6.5–7.0 and a high Km for ThDP (5 mM, suggesting that, in vivo, the rate of AThTP synthesis is proportional to the free ThDP concentration. When ADP was used as the variable substrate at a fixed ThDP concentration, a sigmoid curve was obtained, with a Hill coefficient of 2.1 and an S0.5 value of 0.08 mM. The specificity of the AThTP synthesizing enzyme with respect to nucleotide substrate is restricted to ATP/ADP, and only ThDP can serve as the second substrate of the reaction. We tentatively named this enzyme ThDP adenylyl transferase (EC Conclusion This is the first demonstration of an enzyme activity transferring a nucleotidyl group on thiamine diphosphate to produce AThTP. The existence of a mechanism for the enzymatic synthesis of this compound is in agreement with the hypothesis of a non-cofactor role for thiamine derivatives in living cells.

  9. Quantum theory of tunneling

    CERN Document Server

    Razavy, Mohsen


    In this revised and expanded edition, in addition to a comprehensible introduction to the theoretical foundations of quantum tunneling based on different methods of formulating and solving tunneling problems, different semiclassical approximations for multidimensional systems are presented. Particular attention is given to the tunneling of composite systems, with examples taken from molecular tunneling and also from nuclear reactions. The interesting and puzzling features of tunneling times are given extensive coverage, and the possibility of measurement of these times with quantum clocks are critically examined. In addition by considering the analogy between evanescent waves in waveguides and in quantum tunneling, the times related to electromagnetic wave propagation have been used to explain certain aspects of quantum tunneling times. These topics are treated in both non-relativistic as well as relativistic regimes. Finally, a large number of examples of tunneling in atomic, molecular, condensed matter and ...

  10. Road and Railroad Tunnels (United States)

    Department of Homeland Security — Tunnels in the United States According to the HSIP Tiger Team Report, a tunnel is defined as a linear underground passageway open at both ends. This dataset is based...

  11. Transonic Dynamics Tunnel (TDT) (United States)

    Federal Laboratory Consortium — The Transonic Dynamics Tunnel (TDT) is a continuous flow wind-tunnel facility capable of speeds up to Mach 1.2 at stagnation pressures up to one atmosphere. The TDT...

  12. Hypersonic Tunnel Facility (HTF) (United States)

    Federal Laboratory Consortium — The Hypersonic Tunnel Facility (HTF) is a blow-down, non-vitiated (clean air) free-jet wind tunnel capable of testing large-scale, propulsion systems at Mach 5, 6,...

  13. The Tunnels of Samos (United States)

    Apostol, Tom M. (Editor)


    This 'Project Mathematics' series video from CalTech presents the tunnel of Samos, a famous underground aquaduct tunnel located near the capital of Pithagorion (named after the famed Greek mathematician, Pythagoras, who lived there), on one of the Greek islands. This tunnel was constructed around 600 BC by King Samos and was built under a nearby mountain. Through film footage and computer animation, the mathematical principles and concepts of why and how this aquaduct tunnel was built are explained.

  14. Magnetic tunnel junctions (MTJs)

    Institute of Scientific and Technical Information of China (English)


    We review the giant tunnel magnetoresistance (TMR) in ferromagnetic-insulator-ferromagnetic junctions discovered in recent years, which is the magnetoresistance (MR) associated with the spin-dependent tunneling between two ferromagnetic metal films separated by an insulating thin tunnel barrier. The theoretical and experimental results including junction conductance, magnetoresistance and their temperature and bias dependences are described.

  15. Comparison of plate counts, Petrifilm, dipslides, and adenosine triphosphate bioluminescence for monitoring bacteria in cooling-tower waters. (United States)

    Mueller, Sherry A; Anderson, James E; Kim, Byung R; Ball, James C


    Effective bacterial control in cooling-tower systems requires accurate and timely methods to count bacteria. Plate-count methods are difficult to implement on-site, because they are time- and labor-intensive and require sterile techniques. Several field-applicable methods (dipslides, Petrifilm, and adenosine triphosphate [ATP] bioluminescence) were compared with the plate count for two sample matrices--phosphate-buffered saline solution containing a pure culture of Pseudomonas fluorescens and cooling-tower water containing an undefined mixed bacterial culture. For the pure culture, (1) counts determined on nutrient agar and plate-count agar (PCA) media and expressed as colony-forming units (CFU) per milliliter were equivalent to those on R2A medium (p = 1.0 and p = 1.0, respectively); (2) Petrifilm counts were not significantly different from R2A plate counts (p = 0.99); (3) the dipslide counts were up to 2 log units higher than R2A plate counts, but this discrepancy was not statistically significant (p = 0.06); and (4) a discernable correlation (r2 = 0.67) existed between ATP readings and plate counts. For cooling-tower water samples (n = 62), (1) bacterial counts using R2A medium were higher (but not significant; p = 0.63) than nutrient agar and significantly higher than tryptone-glucose yeast extract (TGE; p = 0.03) and PCA (p ATP readings and plate counts varied from system to system, was poor (r2 values ranged from ATP method was not sufficiently sensitive to measure counts below approximately 10(4) CFU/mL.

  16. Proton tunneling in solids

    Energy Technology Data Exchange (ETDEWEB)

    Kondo, J.


    The tunneling rate of the proton and its isotopes between interstitial sites in solids is studied theoretically. The phonons and/or the electrons in the solid have two effects on the tunneling phenomenon. First, they suppress the transfer integral between two neighbouring states. Second, they give rise to a finite lifetime of the proton state. Usually the second effect is large and the tunneling probability per unit time (tunneling rate) can be defined. In some cases, however, a coherent tunneling is expected and actually observed. (author)

  17. Radiologically placed tunneled peritoneal catheter in palliation of malignant ascites

    Energy Technology Data Exchange (ETDEWEB)

    Akinci, Devrim; Erol, Bekir; Ciftci, Tuerkmen T. [Hacettepe University, Faculty of Medicine, Department of Radiology, 06100 Ankara (Turkey); Akhan, Okan, E-mail: [Hacettepe University, Faculty of Medicine, Department of Radiology, 06100 Ankara (Turkey)


    The purpose of this study was to evaluate retrospectively the safety and effectiveness of radiologically placed tunneled peritoneal catheter in palliation of malignant ascites. Between July 2005 and June 2009, 41 tunneled peritoneal catheters were placed under ultrasonographic and fluoroscopic guidance in 40 patients (mean age, 55 years; 22 women) who had symptomatic malignant ascites. No procedure related mortality was observed. Major complication occurred in one patient (2.5%) in the form of serious bacterial peritonitis that necessitated catheter removal. Minor complications such as minor bacterial peritonitis, catheter dislodgement, tunnel infection, and catheter blockage occurred in 11 patients (27.5%). The mean duration of survival after catheter placement was 11.8 weeks. All patients expired of their primary malignancies in the follow-up. Radiologically placed tunneled peritoneal catheter is safe and effective in palliation of symptomatic malignant ascites.

  18. Rapid, quantitative determination of bacteria in water. [adenosine triphosphate (United States)

    Chappelle, E. W.; Picciolo, G. L.; Thomas, R. R.; Jeffers, E. L.; Deming, J. W. (Inventor)


    A bioluminescent assay for ATP in water borne bacteria is made by adding nitric acid to a water sample with concentrated bacteria to rupture the bacterial cells. The sample is diluted with sterile, deionized water, then mixed with a luciferase-luciferin mixture and the resulting light output of the bioluminescent reaction is measured and correlated with bacteria present. A standard and a blank also are presented so that the light output can be correlated to bacteria in the sample and system noise can be substracted from the readings. A chemiluminescent assay for iron porphyrins in water borne bacteria is made by adding luminol reagent to a water sample with concentrated bacteria and measuring the resulting light output of the chemiluminescent reaction.

  19. Determination of Adenosine Triphosphate on Marine Particulates:Synthesis of Methods for Use on OTEC Samples

    Energy Technology Data Exchange (ETDEWEB)

    Jones, Anthony T.; Hartwig, Eric O.


    Adenosine triphosphate (ATP) is an indicator of living biomass in marine particulates. This report details the method used by Lawrence Berkeley Laboratory to analyze particulate ATP in samples taken from oligotrophic, tropical ocean waters. It represents a synthesis of previously published methods.

  20. Determination of adenosine triphosphate on marine particulates: synthesis of methods for use on OTEC samples

    Energy Technology Data Exchange (ETDEWEB)

    Jones, A.T.; Hartwig, E.O.


    Adenosine triphosphate (ATP) is an indicator of living biomass in marine particulates. This report details the method used by Lawrence Berkeley Laboratory to analyze particulate ATP in samples taken from oligotrophic, tropical ocean waters. It represents a synthesis of previously published methods.

  1. Unexpected Discovery of Dichloroacetate Derived Adenosine Triphosphate Competitors Targeting Pyruvate Dehydrogenase Kinase To Inhibit Cancer Proliferation. (United States)

    Zhang, Shao-Lin; Hu, Xiaohui; Zhang, Wen; Tam, Kin Yip


    Pyruvate dehydrogenase kinases (PDKs) have recently emerged as an attractive target for cancer therapy. Herein, we prepared a series of compounds derived from dichloroacetate (DCA) which inhibited cancer cells proliferation. For the first time, we have successfully developed DCA derived inhibitors that preferentially bind to the adenosine triphosphate (ATP) pocket of PDK isoform 1 (PDK1).

  2. Resonance Enhanced Tunneling

    CERN Document Server

    Matsumoto, S; Matsumoto, Sh.


    Time evolution of tunneling in thermal medium is examined using the real-time semiclassical formalism previously developed. Effect of anharmonic terms in the potential well is shown to give a new mechanism of resonance enhanced tunneling. If the friction from environment is small enough, this mechanism may give a very large enhancement for the tunneling rate. The case of the asymmetric wine bottle potential is worked out in detail.

  3. Ulnar tunnel syndrome. (United States)

    Bachoura, Abdo; Jacoby, Sidney M


    Ulnar tunnel syndrome could be broadly defined as a compressive neuropathy of the ulnar nerve at the level of the wrist. The ulnar tunnel, or Guyon's canal, has a complex and variable anatomy. Various factors may precipitate the onset of ulnar tunnel syndrome. Patient presentation depends on the anatomic zone of ulnar nerve compression: zone I compression, motor and sensory signs and symptoms; zone II compression, isolated motor deficits; and zone III compression; purely sensory deficits. Conservative treatment such as activity modification may be helpful, but often, surgical exploration of the ulnar tunnel with subsequent ulnar nerve decompression is indicated.

  4. Microsystem Aeromechanics Wind Tunnel (United States)

    Federal Laboratory Consortium — The Microsystem Aeromechanics Wind Tunnel advances the study of fundamental flow physics relevant to micro air vehicle (MAV) flight and assesses vehicle performance...

  5. Bacterial Vaginosis (United States)

    ... Issues > Conditions > Sexually Transmitted > Bacterial Vaginosis Health Issues Listen Español Text Size Email Print Share Bacterial Vaginosis Page Content Bacterial vaginosis (BV) is the most common vaginal infection in sexually active teenaged girls . It appears to be caused by ...

  6. Tunnel fire dynamics

    CERN Document Server

    Ingason, Haukur; Lönnermark, Anders


    This book covers a wide range of issues in fire safety engineering in tunnels, describes the phenomena related to tunnel fire dynamics, presents state-of-the-art research, and gives detailed solutions to these major issues. Examples for calculations are provided. The aim is to significantly improve the understanding of fire safety engineering in tunnels. Chapters on fuel and ventilation control, combustion products, gas temperatures, heat fluxes, smoke stratification, visibility, tenability, design fire curves, heat release, fire suppression and detection, CFD modeling, and scaling techniques all equip readers to create their own fire safety plans for tunnels. This book should be purchased by any engineer or public official with responsibility for tunnels. It would also be of interest to many fire protection engineers as an application of evolving technical principles of fire safety.

  7. Increased deoxythymidine triphosphate levels is a feature of relative cognitive decline

    DEFF Research Database (Denmark)

    Madsen, Claus Desler; Frederiksen, Jane H; Olsen, Maria Nathalie Angleys;


    Mitochondrial bioenergetics, mitochondrial reactive oxygen species (ROS) and cellular levels of nucleotides have been hypothesized as early indicators of Alzheimer's disease (AD). Utilizing relative decline of cognitive ability as a predictor of AD risk, we evaluated the correlation between change...... of deoxythymidine-triphosphate (dTTP) (20%), but not mitochondrial bioenergetics parameters measured in this study or mitochondrial ROS. Levels of dTTP in PBMCs are indicators of relative cognitive change suggesting a role of deoxyribonucleotides in the etiology of AD....... of deoxyribonucleotide triphosphates were measured in peripheral blood mononuclear cells (PBMCs) from a total of 103 selected participants displaying the most pronounced relative cognitive decline and relative cognitive improvement. We show that relative cognitive decline is associated with higher PBMC content...

  8. Microcontroller-assisted compensation of adenosine triphosphate levels: instrument and method development. (United States)

    Hu, Jie-Bi; Chen, Ting-Ru; Chen, Yu-Chie; Urban, Pawel L


    In order to ascertain optimum conditions for biocatalytic processes carried out in vitro, we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP concentration range of 2-48 μM. The report demonstrates feasibility of the device operation using apyrase as the ATP-depleting enzyme.

  9. Description of a novel eukaryotic deoxyuridine 5'-triphosphate nucleotidohydrolase in Leishmania major

    DEFF Research Database (Denmark)

    Camacho, A; Arrebola, R; Pena Diaz, Javier;


    . None of the characteristic motifs were readily identifiable and the sequence encoded a larger polypeptide. However, the products of the reaction were dUMP and PPi, competition experiments with other deoxyribonucleoside triphosphates showed that the reaction is specific for dUTP, and the protozoan gene...... of recombinant enzyme in large quantities will now permit detailed mechanistic and structural studies, which might contribute to a rational design of specifically targeted inhibitors against dUTPase from L. major....

  10. Ultrafast scanning tunneling microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Botkin, D.A. [California Univ., Berkeley, CA (United States). Dept. of Physics]|[Lawrence Berkeley Lab., CA (United States)


    I have developed an ultrafast scanning tunneling microscope (USTM) based on uniting stroboscopic methods of ultrafast optics and scanned probe microscopy to obtain nanometer spatial resolution and sub-picosecond temporal resolution. USTM increases the achievable time resolution of a STM by more than 6 orders of magnitude; this should enable exploration of mesoscopic and nanometer size systems on time scales corresponding to the period or decay of fundamental excitations. USTM consists of a photoconductive switch with subpicosecond response time in series with the tip of a STM. An optical pulse from a modelocked laser activates the switch to create a gate for the tunneling current, while a second laser pulse on the sample initiates a dynamic process which affects the tunneling current. By sending a large sequence of identical pulse pairs and measuring the average tunnel current as a function of the relative time delay between the pulses in each pair, one can map the time evolution of the surface process. USTM was used to measure the broadband response of the STM`s atomic size tunnel barrier in frequencies from tens to hundreds of GHz. The USTM signal amplitude decays linearly with the tunnel junction conductance, so the spatial resolution of the time-resolved signal is comparable to that of a conventional STM. Geometrical capacitance of the junction does not appear to play an important role in the measurement, but a capacitive effect intimately related to tunneling contributes to the measured signals and may limit the ultimate resolution of the USTM.

  11. Regulation of adenosine triphosphate-sensitive potassium channels suppresses the toxic effects of amyloid-beta peptide (25-35)

    Institute of Scientific and Technical Information of China (English)

    Min Kong; Maowen Ba; Hui Liang; Peng Shao; Tianxia Yu; Ying Wang


    In this study, we treated PC12 cells with 0-20 μM amyloid-β peptide (25-35) for 24 hours to induce cytotoxicity, and found that 5-20 μM amyloid-β peptide (25-35) decreased PC12 cell viability, but adenosine triphosphate-sensitive potassium channel activator diazoxide suppressed the decrease reactive oxygen species levels. These protective effects were reversed by the selective mitochondrial adenosine triphosphate-sensitive potassium channel blocker 5-hydroxydecanoate. An inducible nitric oxide synthase inhibitor, Nω-nitro-L-arginine, also protected PC12 cells from intracellular reactive oxygen species levels. However, the H2O2-degrading enzyme catalase could that the increases in both mitochondrial membrane potential and reactive oxygen species levels adenosine triphosphate-sensitive potassium channels and nitric oxide. Regulation of adenosine triphosphate-sensitive potassium channels suppresses PC12 cell cytotoxicity induced by amyloid-β

  12. Structural and functional insights into DR2231 protein, the MazG-like nucleoside triphosphate pyrophosphohydrolase from Deinococcus radiodurans. (United States)

    Gonçalves, Ana Maria D; de Sanctis, Daniele; McSweeney, Sean M


    Deinococcus radiodurans is among the very few bacterial species extremely resistant to ionizing radiation, UV light, oxidizing agents, and cycles of prolonged desiccation. The proteome of D. radiodurans reflects the evolutionary pressure exerted by chronic exposure to (nonradioactive) forms of DNA and protein damage. A clear example of this adaptation is the overrepresentation of protein families involved in the removal of non-canonical nucleoside triphosphates (NTPs) whose incorporation into nascent DNA would promote mutagenesis and DNA damage. The three-dimensional structure of the DR2231 protein has been solved at 1.80 Å resolution. This protein had been classified as an all-α-helical MazG-like protein. The present study confirms that it holds the basic structural module characteristic of the MazG superfamily; two helices form a rigid domain, and two helices form a mobile domain and connecting loops. Contrary to what is known of MazG proteins, DR2231 protein shows a functional affinity with dUTPases. Enzymatic and isothermal calorimetry assays have demonstrated high specificity toward dUTP but an inability to hydrolyze dTTP, a typical feature of dUTPases. Co-crystallization with the product of hydrolysis, dUMP, in the presence of magnesium or manganese cations, suggests similarities with the dUTP/dUDP hydrolysis mechanism reported for dimeric dUTPases. The genome of D. radiodurans encodes for all enzymes required for dTTP synthesis from dCMP, thus bypassing the need of a dUTPase. We postulate that DR2231 protein is not essential to D. radiodurans and rather performs "house-cleaning" functions within the framework of oxidative stress response. We further propose DR2231 protein as an evolutionary precursor of dimeric dUTPases.

  13. Lack of correlation between Legionella colonization and microbial population quantification using heterotrophic plate count and adenosine triphosphate bioluminescence measurement. (United States)

    Duda, Scott; Baron, Julianne L; Wagener, Marilyn M; Vidic, Radisav D; Stout, Janet E


    This investigation compared biological quantification of potable and non-potable (cooling) water samples using pour plate heterotrophic plate count (HPC) methods and adenosine triphosphate (ATP) concentration measurement using bioluminescence. The relationship between these measurements and the presence of Legionella spp. was also examined. HPC for potable and non-potable water were cultured on R2A and PCA, respectively. Results indicated a strong correlation between HPC and ATP measurements in potable water (R = 0.90, p ATP and HPC were much weaker but statistically significant (make-up water: R = 0.37, p = 0.005; cooling tower 1: R = 0.52, p ATP. However, ATP measurements showed higher microbial concentrations than HPC measurements. Following chlorination of the cooling towers, ATP measurements indicated very low bacterial concentrations (1000 CFU/mL) which consisted primarily of non-tuberculous mycobacteria. HPC concentrations have been suggested to be predictive of Legionella presence, although this has not been proven. Our evaluation showed that HPC or ATP demonstrated a fair predictive capacity for Legionella positivity in potable water (HPC: receiver operating characteristic (ROC) = 0.70; ATP: ROC = 0.78; p = 0.003). However, HPC or ATP correctly classified sites as positive only 64 and 62% of the time, respectively. No correlation between HPC or ATP and Legionella colonization in non-potable water samples was found (HPC: ROC = 0.28; ATP: ROC = 0.44; p = 0.193).

  14. Virtual photons in macroscopic tunneling


    Aichmann, Horst; Nimtz, Guenter; Bruney, Paul


    Tunnelling processes are thought to proceed via virtual waves due to observed superluminal (faster than light) signal speeds. Some assume such speeds must violate causality. These assumptions contradict, for instance, superluminally tunnelled music and optical tunnelling couplers applied in fiber communication. Recently tunnelling barriers were conjectured to be cavities, wherein the tunnelled output signal is not causally related with the input. The tests described here resolve that tunnelli...

  15. Wind Tunnel Facility (United States)

    Federal Laboratory Consortium — This ARDEC facility consists of subsonic, transonic, and supersonic wind tunnels to acquire aerodynamic data. Full-scale and sub-scale models of munitions are fitted...

  16. Wind Tunnel Testing Facilities (United States)

    Federal Laboratory Consortium — NASA Ames Research Center is pleased to offer the services of our premier wind tunnel facilities that have a broad range of proven testing capabilities to customers...

  17. Water Tunnel Facility (United States)

    Federal Laboratory Consortium — NETL’s High-Pressure Water Tunnel Facility in Pittsburgh, PA, re-creates the conditions found 3,000 meters beneath the ocean’s surface, allowing scientists to study...


    Directory of Open Access Journals (Sweden)

    Florin MUNTEANU


    Full Text Available The 1.2 m x 1.2 m Trisonic Blowdown Wind Tunnel is the largest of the experimental facilities at the National Institute for Aerospace Research - I.N.C.A.S. "Elie Carafoli", Bucharest, Romania. The tunnel has been designed by the Canadian company DSMA (now AIOLOS and since its commissioning in 1978 has performed high speed aerodynamic tests for more than 120 projects of aircraft, missiles and other objects among which the twin jet fighter IAR-93, the jet trainer IAR-99, the MIG-21 Lancer, the Polish jet fighter YRYDA and others. In the last years the wind tunnel has been used mostly for experimental research in European projects such as UFAST. The high flow quality parameters and the wide range of testing capabilities ensure the competitivity of the tunnel at an international level.

  19. Counter Tunnel Project (United States)


    the point clouds . This counter-tunnel effort was funded by the Joint Ground Robotics Enterprise (JGRE) from fiscal year 2010 through 2013, run...slide images against an iterative closest point (ICP) algorithm initialized using the centroids of the point clouds . The slide image algorithm performed...unnecessary for most of the time in the tunnels. However, the 3D point clouds made it possible to measure the height of objects lying in the path, detect

  20. Gravity Tunnel Drag

    CERN Document Server

    Concannon, Thomas


    The time it takes to fall down a tunnel through the center of the Earth to the other side takes approximately 42 minutes, but only when given several simplifying assumptions: a uniform density Earth; a gravitational field that varies linearly with radial position; a non-rotating Earth; a tunnel evacuated of air; and zero friction along the sides of the tunnel. Though several papers have singularly relaxed the first three assumptions, in this paper we relax the final two assumptions and analyze the motion of a body experiencing these types of drag forces in the tunnel. Under such drag forces, we calculate the motion of a transport vehicle through a tunnel of the Earth under uniform density, under constant gravitational acceleration, and finally under the more realistic Preliminary Reference Earth Model (PREM) density data. We find the density profile corresponding to a constant gravitational acceleration better models the motion through the tunnel compared to the PREM density profile, and the uniform density m...

  1. Using an Adenosine Triphosphate Bioluminescent Assay to Determine Effective Antibiotic Combinations against Carbapenem-Resistant Gram Negative Bacteria within 24 Hours.

    Directory of Open Access Journals (Sweden)

    Yiying Cai

    Full Text Available Current in vitro combination testing methods involve enumeration by bacterial plating, which is labor-intensive and time-consuming. Measurement of bioluminescence, released when bacterial adenosine triphosphate binds to firefly luciferin-luciferase, has been proposed as a surrogate for bacterial counts. We developed an ATP bioluminescent combination testing assay with a rapid turnaround time of 24h to determine effective antibiotic combinations.100 strains of carbapenem-resistant (CR GNB [30 Acinetobacter baumannii (AB, 30 Pseudomonas aeruginosa (PA and 40 Klebsiella pneumoniae (KP] were used. Bacterial suspensions (105 CFU/ml were added to 96-well plates containing clinically achievable concentrations of multiple single and two-antibiotic combinations. At 24h, the luminescence intensity of each well was measured. Receiver operator characteristic curves were plotted to determine optimal luminescence threshold (TRLU to discriminate between inhibitory/non-inhibitory combinations when compared to viable plating. The unweighted accuracy (UA [(sensitivity + specificity/2] of TRLU values was determined. External validation was further done using 50 additional CR-GNB.Predictive accuracies of TRLU were high for when all antibiotic combinations and species were collectively analyzed (TRLU = 0.81, UA = 89%. When individual thresholds for each species were determined, UA remained high. Predictive accuracy was highest for KP (TRLU = 0.81, UA = 91%, and lowest for AB (TRLU = 0.83, UA = 87%. Upon external validation, high overall accuracy (91% was observed. The assay distinguished inhibitory/non-inhibitory combinations with UA of 80%, 94% and 93% for AB, PA and KP respectively.We developed an assay that is robust at identifying useful combinations with a rapid turn-around time of 24h, and may be employed to guide the timely selection of effective antibiotic combinations.

  2. Suppression of tunneling rate fluctuations in tunnel field-effect transistors by enhancing tunneling probability (United States)

    Mori, Takahiro; Migita, Shinji; Fukuda, Koichi; Asai, Hidehiro; Morita, Yukinori; Mizubayashi, Wataru; Liu, Yongxun; O’uchi, Shin-ichi; Fuketa, Hiroshi; Otsuka, Shintaro; Yasuda, Tetsuji; Masahara, Meishoku; Ota, Hiroyuki; Matsukawa, Takashi


    This paper discusses the impact of the tunneling probability on the variability of tunnel field-effect transistors (TFETs). Isoelectronic trap (IET) technology, which enhances the tunneling current in TFETs, is used to suppress the variability of the ON current and threshold voltage. The simulation results show that suppressing the tunneling rate fluctuations results in suppression of the variability. In addition, a formula describing the relationship between the tunneling rate fluctuations and the electric field strength is derived based on Kane’s band-to-band tunneling model. This formula indicates that the magnitude of the tunneling rate fluctuations is proportional to the magnitude of the fluctuations in the electric field strength and a higher tunneling probability results in a lower variability. The derived relationship is universally valid for any technologies that exploit enhancement of the tunneling probability, including IET technology, channel material engineering, heterojunctions, strain engineering, etc.

  3. Estimation of adenosine triphosphate utilization of rat mast cells during and after anaphylactic histamine secretion

    DEFF Research Database (Denmark)

    Johansen, Torben


    Determination of the cellular content of adenosine triphosphate (ATP) and the rate of ATP-synthesis were used to estimate the cellular utilization of ATP in relation to anaphylactic histamine secretion. There was an increased rate of oxidative ATP-synthesis and a decreased cellular ATP content...... during the time period of histamine secretion and immediately after its completion. During secretion the additional ATP-utilization above the basal level of ATP-synthesis was 0.51 pmol/10(3) cells. 2.5 min after cell activation, the rate of additional ATP-utilization was 0.30 pmol/10(3) cells...

  4. Enhanced Diffusion of Molecular Motors in the Presence of Adenosine Triphosphate and External Force (United States)

    Shinagawa, Ryota; Sasaki, Kazuo


    The diffusion of a molecular motor in the presence of a constant external force is considered on the basis of a simple theoretical model. The motor is represented by a Brownian particle moving in a series of parabolic potentials placed periodically on a line, and the potential is switched stochastically from one parabola to another by a chemical reaction, which corresponds to the hydrolysis or synthesis of adenosine triphosphate (ATP) in motor proteins. It is found that the diffusion coefficient as a function of the force exhibits peaks. The mechanism of this diffusion enhancement and the possibility of observing it in F1-ATPase, a biological rotary motor, are discussed.

  5. [An adenosine triphosphate bioluminescence assay for detecting the number of living cells]. (United States)

    Liu, S; Peng, Z; Wang, H; Lou, J; He, B; Tang, Q; Qiu, D


    The method for detecting the number of living cells was studied. Using an adenosine triphosphate (ATP) bioluminescence assay, the present authors reported a perfect linear relationship between lg ATP concentrations and lg luminescence counts (r = 0.9963) as well as a relationship between lg number of cells and lg ATP luminescence counts (r = 0.9922). The detectable cells ranged from 10(2) to 10(6) cells/ml, the coefficients of variation 1-3%. This method is simple, accurate and sensitive and has a high reproducibility.

  6. Bacterial gastroenteritis (United States)

    Bacterial gastroenteritis is present when bacteria cause an infection of the stomach and intestines ... has not been treated Many different types of bacteria can cause ... Campylobacter jejuni E coli Salmonella Shigella Staphylococcus ...

  7. Femtosecond scanning tunneling microscope

    Energy Technology Data Exchange (ETDEWEB)

    Taylor, A.J.; Donati, G.P.; Rodriguez, G.; Gosnell, T.R.; Trugman, S.A.; Some, D.I.


    This is the final report of a three-year, Laboratory Directed Research and Development (LDRD) project at the Los Alamos National Laboratory (LANL). By combining scanning tunneling microscopy with ultrafast optical techniques we have developed a novel tool to probe phenomena on atomic time and length scales. We have built and characterized an ultrafast scanning tunneling microscope in terms of temporal resolution, sensitivity and dynamic range. Using a novel photoconductive low-temperature-grown GaAs tip, we have achieved a temporal resolution of 1.5 picoseconds and a spatial resolution of 10 nanometers. This scanning tunneling microscope has both cryogenic and ultra-high vacuum capabilities, enabling the study of a wide range of important scientific problems.

  8. Early times in tunneling

    CERN Document Server

    García-Calderón, G; Garcia-Calderon, Gaston; Villavicencio, Jorge


    Exact analytical solutions of the time-dependent Schr\\"odinger equation with the initial condition of an incident cutoff wave are used to investigate the traversal time for tunneling. The probability density starts from a vanishing value along the tunneling and transmitted regions of the potential. At the barrier width it exhibits, at early times, a distribution of traversal times that typically has a peak $\\tau_p$ and a width $\\Delta \\tau$. Numerical results for other tunneling times, as the phase-delay time, fall within $\\Delta \\tau$. The B\\"uttiker traversal time is the closest to $\\tau_p$. Our results resemble calculations based on Feynman paths if its noisy behaviour is ignored.

  9. Tunneling Through Black Rings

    Institute of Scientific and Technical Information of China (English)

    ZHAO Liu


    Hawking radiation of black ring solutions to 5-dimensional Einstein-Maxwell-dilaton theory is analyzed by use of the Parikh-Wilczek tunneling method. To get the correct tunneling amplitude and emission rate, we adopt and develop the Angheben-Nadalini-Vanzo-Zerbini covariant approach to cover the effects of rotation and electronic discharge all at once, and the effect of back reaction is also taken into account. This constitutes a unified approach to the tunneling problem. Provided the first law of thermodynamics for black rings holds, the emission rate is proportional to the exponential of the change of Bekenstein-Hawking entropy. Explicit calculation for black ring temperatures agrees exactly with the results obtained via the classical surface gravity method and the quasi-local formalism.

  10. Long distance tunneling

    CERN Document Server

    Ivlev, B I


    Quantum tunneling between two potential wells in a magnetic field can be strongly increased when the potential barrier varies in the direction perpendicular to the line connecting the two wells and remains constant along this line. A periodic structure of the wave function is formed in the direction joining the wells. The resulting motion can be coherent like motion in a conventional narrow band periodic structure. A particle penetrates the barrier over a long distance which strongly contrasts to WKB-like tunneling. The whole problem is stationary. The coherent process can be influenced by dissipation.

  11. Tunneling in axion monodromy (United States)

    Brown, Jon; Cottrell, William; Shiu, Gary; Soler, Pablo


    The Coleman formula for vacuum decay and bubble nucleation has been used to estimate the tunneling rate in models of axion monodromy in recent literature. However, several of Coleman's original assumptions do not hold for such models. Here we derive a new estimate with this in mind using a similar Euclidean procedure. We find that there are significant regions of parameter space for which the tunneling rate in axion monodromy is not well approximated by the Coleman formula. However, there is also a regime relevant to large field inflation in which both estimates parametrically agree. We also briefly comment on the applications of our results to the relaxion scenario.

  12. Tunneling in Axion Monodromy

    CERN Document Server

    Brown, Jon; Shiu, Gary; Soler, Pablo


    The Coleman formula for vacuum decay and bubble nucleation has been used to estimate the tunneling rate in models of axion monodromy in recent literature. However, several of Coleman's original assumptions do not hold for such models. Here we derive a new estimate with this in mind using a similar Euclidean procedure. We find that there are significant regions of parameter space for which the tunneling rate in axion monodromy is not well approximated by the Coleman formula. However, there is also a regime relevant to large field inflation in which both estimates parametrically agree. We also briefly comment on the applications of our results to the relaxion scenario.

  13. Breaking through the tranfer tunnel

    CERN Multimedia

    Laurent Guiraud


    This image shows the tunnel boring machine breaking through the transfer tunnel into the LHC tunnel. Proton beams will be transferred from the SPS pre-accelerator to the LHC at 450 GeV through two specially constructed transfer tunnels. From left to right: LHC Project Director, Lyn Evans; CERN Director-General (at the time), Luciano Maiani, and Director for Accelerators, Kurt Hubner.

  14. Electrophysiologic effects of adenosine triphosphate on rabbit sinoatrial node pace maker cells via P1 receptors

    Institute of Scientific and Technical Information of China (English)

    RENLei-Ming; LIJun-Xia; SHIChen-Xia; ZHAODing


    AIM: To study the electrophysiologic effects of adenosine triphosphate (ATP) on rabbit sinoatrial node pacemakercells and the receptors related with the action of ATP. METHODS: Intracellular microelectrode method was usedto record the parameters of action potential (AP) in the rabbit sinoatrial nodes. RESULTS: ATP (0.1-3 mmol/L)decreased the rate of pacemaker firing (RPF) by 16 %-43 % and velocity of diastolic depolarization (VDD) by 33 %-67 %, increased the amplitude of AP (APA) by 6 %-9 % and maximal rate of depolarization (Vmax) by 30 %-76 %,shortened APD50 by 7 %-12 % and APD90 by 6.3 %-9 %, concentration-dependently. The effects of ATP, adenos-ine (Ado), and adenosine diphosphate at the same concentration on AP were not different from each other significantly.Neither uridine triphosphate nor, α,β-methylene ATP had significant electrophysiologic effects on the sinoatrialnode of rabbits. Both the electrophysiologic effects of ATP and Ado on pacemaker cells were inhibited by P1receptor antagonist aminophylline 0.1 mmol/L (P0.05). CONCLUSION: There are nofunctional P2X1 and P2Y2 receptors on pacemaker cells of the rabbit sinoatrial nodes, and the electrophysiologiceffects of ATP in the rabbit sinoatrial node pacemaker cells are mediated via P1 receptors by Ado degraded fromATP.

  15. Intercalation of gaseous thiols and sulfides into Ag+ ion-exchanged aluminum dihydrogen triphosphate. (United States)

    Hayashi, Aki; Saimen, Hiroki; Watanabe, Nobuaki; Kimura, Hitomi; Kobayashi, Ayumi; Nakayama, Hirokazu; Tsuhako, Mitsutomo


    Ag(+) ion-exchanged layered aluminum dihydrogen triphosphate (AlP) with the interlayer distance of 0.85 nm was synthesized by the ion-exchange of proton in triphosphate with Ag(+) ion. The amount of exchanged Ag(+) ion depended on the concentration of AgNO(3) aqueous solution. Ag(+) ion-exchanged AlP adsorbed gaseous thiols and sulfides into the interlayer region. The adsorption amounts of thiols were more than those of sulfides, thiols with one mercapto group > thiol with two mercapto groups > sulfides, and depended on the amount of exchanged Ag(+) ion in the interlayer region. The thiols with one mercapto group were intercalated to expand the interlayer distance of Ag(+) ion-exchanged AlP, whereas there was no expansion in the adsorption of sulfide. In the case of thiol with two mercapto groups, there was observed contraction of the interlayer distance through the bridging with Ag(+) ions of the upper and lower sides of the interlayer region.

  16. Encapsulation of antiviral nucleotide analogues azidothymidine-triphosphate and cidofovir in poly(iso-butylcyanoacrylate) nanocapsules. (United States)

    Hillaireau, H; Le Doan, T; Besnard, M; Chacun, H; Janin, J; Couvreur, P


    Nucleoside analogues are widely used in the treatment of various viral infections. However, the poor in vivo conversion of the nucleoside analogues like azidothymidine (AZT) into their active triphosphate nucleotide counterpart limits their pharmacological efficacy. This could be overcome by the direct administration of azidothymidine triphosphate (AZT-TP), but it requires an appropriate drug delivery approach. Besides nucleoside analogues, nucleotide analogues like cidofovir (CDV) are also used in the treatment of viral infections. CDV has raised recent interest because of its promising activity against smallpox, but its use is limited by its poor bioavailability and nephrotoxicity. Here again, a proper drug delivery system should address these issues. In this study, we investigated the encapsulation of the nucleotide analogues AZT-TP and CDV into poly(iso-butylcyanoacrylate) aqueous core nanocapsules, known to efficiently entrap oligonucleotides. We show here that the encapsulation of these mono-nucleotides is less efficient than with oligonucleotides and that a rapid release of AZT-TP from the nanocapsules occurred in vitro. This highlights the importance of the molecular weight of the entrapped molecules which, if they are too small, are diffusing through the thin polymer membrane of the nanocapsules. On the other hand, a good protection of the encapsulated AZT-TP was observed.


    Directory of Open Access Journals (Sweden)

    Corneliu STOICA


    Full Text Available The INCAS Subsonic Wind Tunnel is a closed circuit, continuous, atmospheric pressure facility with a maximum speed of 110 m/s. The test section is octagonal ,of 2.5 m wide, 2.0 m high and 4 m long. The tunnel is powered by a 1200 kW, air cooled variable speed DC motor which drives a 12 blade, 3.5 m diameter fan and is equipped with a six component pyramidal type external mechanical balance with a 700 Kgf maximum lift capacity.The angle of attack range is between -45º and +45º while the yaw angle range is between -140º and +216º .The data acquisition system has been modified recently to allow the recording of all test data on a PC - type computer using LABVIEW and a PXI – type chassis containing specialized data acquisition modules.The tunnel is equipped with a variable frequency electrical supply system for powered models and a 10 bar compressed air supply for pneumatic flow control applications.In the recent years the subsonic wind tunnel has been intensively used for tests within several European projects (AVERT, CESAR and others.

  18. Classical trajectories and quantum tunneling

    CERN Document Server

    Ivlev, B I


    The problem of inter-band tunneling in a semiconductor (Zener breakdown) in a nonstationary and homogeneous electric field is solved exactly. Using the exact analytical solution, the approximation based on classical trajectories is studied. A new mechanism of enhanced tunneling through static non-one-dimensional barriers is proposed in addition to well known normal tunneling solely described by a trajectory in imaginary time. Under certain conditions on the barrier shape and the particle energy, the probability of enhanced tunneling is not exponentially small even for non-transparent barriers, in contrast to the case of normal tunneling.

  19. Comparison of immunomagnetic separation/adenosine triphosphate rapid method to traditional culture-based method for E. coli and enterococci enumeration in wastewater (United States)

    Bushon, R.N.; Likirdopulos, C.A.; Brady, A.M.G.


    Untreated wastewater samples from California, North Carolina, and Ohio were analyzed by the immunomagnetic separation/adenosine triphosphate (IMS/ATP) method and the traditional culture-based method for E. coli and enterococci concentrations. The IMS/ATP method concentrates target bacteria by immunomagnetic separation and then quantifies captured bacteria by measuring bioluminescence induced by release of ATP from the bacterial cells. Results from this method are available within 1 h from the start of sample processing. Significant linear correlations were found between the IMS/ATP results and results from traditional culture-based methods for E. coli and enterococci enumeration for one location in California, two locations in North Carolina, and one location in Ohio (r??values ranged from 0.87 to 0.97). No significant linear relation was found for a second location in California that treats a complex mixture of residential and industrial wastewater. With the exception of one location, IMS/ATP showed promise as a rapid method for the quantification of faecal-indicator organisms in wastewater.

  20. Comparison of immunomagnetic separation/adenosine triphosphate rapid method to traditional culture-based method for E. coli and enterococci enumeration in wastewater. (United States)

    Bushon, Rebecca N; Likirdopulos, Christina A; Brady, Amie M G


    Untreated wastewater samples from California, North Carolina, and Ohio were analyzed by the immunomagnetic separation/adenosine triphosphate (IMS/ATP) method and the traditional culture-based method for E. coli and enterococci concentrations. The IMS/ATP method concentrates target bacteria by immunomagnetic separation and then quantifies captured bacteria by measuring bioluminescence induced by release of ATP from the bacterial cells. Results from this method are available within 1h from the start of sample processing. Significant linear correlations were found between the IMS/ATP results and results from traditional culture-based methods for E. coli and enterococci enumeration for one location in California, two locations in North Carolina, and one location in Ohio (r values ranged from 0.87 to 0.97). No significant linear relation was found for a second location in California that treats a complex mixture of residential and industrial wastewater. With the exception of one location, IMS/ATP showed promise as a rapid method for the quantification of faecal-indicator organisms in wastewater.

  1. Bacterial Adhesion & Blocking Bacterial Adhesion

    DEFF Research Database (Denmark)

    Vejborg, Rebecca Munk


    tract to the microbial flocs in waste water treatment facilities. Microbial biofilms may however also cause a wide range of industrial and medical problems, and have been implicated in a wide range of persistent infectious diseases, including implantassociated microbial infections. Bacterial adhesion...... is the first committing step in biofilm formation, and has therefore been intensely scrutinized. Much however, still remains elusive. Bacterial adhesion is a highly complex process, which is influenced by a variety of factors. In this thesis, a range of physico-chemical, molecular and environmental parameters......, which influence the transition from a planktonic lifestyle to a sessile lifestyle, have been studied. Protein conditioning film formation was found to influence bacterial adhesion and subsequent biofilm formation considerable, and an aqueous extract of fish muscle tissue was shown to significantly...

  2. Bacterial lipases

    NARCIS (Netherlands)

    Jaeger, Karl-Erich; Ransac, Stéphane; Dijkstra, Bauke W.; Colson, Charles; Heuvel, Margreet van; Misset, Onno


    Many different bacterial species produce lipases which hydrolyze esters of glycerol with preferably long-chain fatty acids. They act at the interface generated by a hydrophobic lipid substrate in a hydrophilic aqueous medium. A characteristic property of lipases is called interfacial activation, mea

  3. Bacterial Ecology

    DEFF Research Database (Denmark)

    Fenchel, Tom


    Bacterial ecology is concerned with the interactions between bacteria and their biological and nonbiological environments and with the role of bacteria in biogeochemical element cycling. Many fundamental properties of bacteria are consequences of their small size. Thus, they can efficiently exploit...

  4. Adenosine triphosphate concentration in relation to microbial biomass in aquatic systems

    Energy Technology Data Exchange (ETDEWEB)

    Cunningham, H.W. Jr.


    Analyses of adenosine triphosphate (ATP) extracted from a sediment community by the sulfuric acid method are complicated by inhibitions from inorganic and organic compounds. Inhibitions by inorganic compounds are reversible while those by organic compounds are irreversible. The primary inhibition by organic compounds results by complexing with acid-soluble fulvic acids which will prevent the detection of as much as 80% of the ATP present in a sample by the luciferin-luciferase reaction. Analytical techniques were developed to parially circumvent such interferences. Biomass interpretations from ATP concentrations in aquatic systems are complicated by the diversity of the microbiota and by the variability in the carbon to ATP ratio caused by environmental conditions. However, when levels of ATP are considered as a physiological condition of a sedimentary community, this data provide a means to interpret community metabolism not available hitherto.

  5. Adenosine triphosphate concentration in relation to microbial biomass in aquatic systems

    Energy Technology Data Exchange (ETDEWEB)

    Cunningham, H.W. Jr.


    Analyses of adenosine triphosphate (ATP) extracted from a sediment community of an aquatic ecosystem by the sulfuric acid method are complicated by inhibitions from inorganic and organic compounds. Inhibitions by inorganic compounds are reversible while those by organic compounds are irreversible. The primary inhibition by organic compounds results by complexing with acid-soluble fulvic acids which will prevent the detection of as much as 80% of the ATP present in a sample by the luciferin-luciferase reaction. Analytical techniques were developed to partially circumvent such interferences. Biomass interpretations from ATP concentrations in aquatic systems are complicated by the diversity of the microbiota and by the variability in the carbon to ATP ratio caused by environmental conditions. However, when levels of ATP are considered as a physiological condition of a sedimentary community, this data provides a means to interpret community metabolism not available hitherto.

  6. A Destabilized Case of Stable Effort Angina Pectoris Induced by Low-dose Adenosine Triphosphate (United States)

    Sueta, Daisuke; Kojima, Sunao; Izumiya, Yasuhiro; Yamamuro, Megumi; Kaikita, Koichi; Hokimoto, Seiji; Ogawa, Hisao


    A 79-year-old man was diagnosed with sudden deafness. He had previously experienced a suspected episode of angina pectoris. At a local hospital, after 500 mg of hydrocortisone and 80 mg adenosine triphosphate (ATP) were administered, he became aware of chest discomfort. An electrocardiogram revealed serious ST-segment depressions. He was diagnosed with a non-ST elevated myocardial infarction (NSTEMI). Emergency coronary angiography revealed triple vessel disease, and the lesion was successfully stented. The mechanisms whereby the stable effort angina pectoris destabilized in this case were thought to include a reduction of the local blood flow because of an ATP product and probable thrombus formation in response to the administered steroids. PMID:27853071

  7. Interaction of Divalent Metal Ions with the Adenosine Triphosphate Measured Using Nuclear Magnetic Resonance

    Institute of Scientific and Technical Information of China (English)


    The interaction of adenosine triphosphate with divalent metal ions is important in biochemical functions. The effects of pH and metal ions Mg2+, Ca2+, Zn2+, Mn2+, and Co2+ on the chemical shift of the phosphate group of ATP have been studied using Nuclear Magnetic Resonance. The chemical shift of the β-phosphate of ATP is the most sensitive to pH. Ca2+ and Mg2+ bind with the α- and β-phosphate groups of ATP. Zn2+ binds to the adenosine ring hydrogen as well as to phosphate. The paramagnetic ions Mn2+ and Co2+ do not cause chemical shifts of the phosphate or proton peak. Mn2+ and Co2+ broaden the resonance peak only.

  8. Genetics and complementation of Haemophilus influenzae mutants deficient in adenosine 5'-triphosphate-dependent nuclease

    Energy Technology Data Exchange (ETDEWEB)

    Kooistra, J.; Small, G.D.; Setlow, J.K.; Shapanka, R.


    Eight different mutations in Haemophilus influenzae leading to deficiency in adenosine 5'-triphosphate (ATP)-dependent nuclease have been investigated in strains in which the mutations of the originally mutagenized strains have been transferred into the wild type. Sensitivity to mitomycin C and deoxycholate and complementation between extracts and deoxyribonucleic acid (DNA)-dependent ATPase activity have been measured. Genetic crosses have provided information on the relative position of the mutations on the genome. There are three complementation groups, corresponding to three genetic groups. The strains most sensitive to mitomycin and deoxycholate, derived from mutants originally selected on the basis of sensitivity to mitomycin C or methyl methanesulfonate, are in one group. Apparently all these sensitive strains lack DNA-dependent ATPase activity, as does a strain intermediate in sensitivity to deoxycholate, which is the sole representative of another group. There are four strains that are relatively resistant to deoxycholate and mitomycin C, and all of these contain the ATPase activity.

  9. Localization of RNA transcription sites in insect oocytes using microinjections of 5-bromouridine 5'-triphosphate.

    Directory of Open Access Journals (Sweden)

    Dmitry Bogolyubov


    Full Text Available In the present study we used 5-bromouridine 5'-triphosphate (BrUTP microinjections to localize the transcription sites in oocytes of insects with different types of the ovarium structure: panoistic, meroistic polytrophic, and meroistic telotrophic. We found that in an insect with panoistic ovaries (Acheta domesticus, oocyte nuclei maintain their transcription activity during the long period of oocyte growth. In insects with meroistic ovaries (Tenebrio molitor and Panorpa communis, early oocyte chromosomes were found to be transcriptionally active, and some transcription activity still persist while the karyosphere, a compact structure formed by all condensed oocyte chromosomes, begins to develop. At the latest stages of karyosphere development, no anti-Br-RNA signal was registered in the karyosphere.

  10. Educational Wind Tunnel

    Directory of Open Access Journals (Sweden)

    Juozas Bielskus


    Full Text Available The paper analyzes an educational wind tunnel produced by the Department of Building Energetics (DBE of Vilnius Gediminas Technical University. The equipment could be used for performing laboratory works and simple research. The article presents the projection of inflow and outlet velocity in the working chamber of DBE wind tunnel and carries out actual noise level measurement. The received data are compared with information on the level of noise generated by the fan considering instructions provided by the manufacturer. In order to assess the reliability of the computer program, simulation applying PHOENICS software has been conducted. The aim of modeling is to simulate a pilot model and to compare the obtained results with those of an analogous test presented in scientific articles.Article in Lithuanian

  11. Programmable ferroelectric tunnel memristor

    Directory of Open Access Journals (Sweden)

    Andy eQuindeau


    Full Text Available We report an analogously programmable memristor based on genuine electronic resistive switching combining ferroelectric switching and electron tunneling. The tunnel current through an 8 unit cell thick epitaxial Pb(Zr[0.2]Ti[0.8]O[3] film sandwiched between La[0.7]Sr[0.3]MnO[3] and cobalt electrodes obeys the Kolmogorov-Avrami-Ishibashi model for bidimensional growth with a characteristic switching time in the order of 10^-7 seconds. The analytical description of switching kinetics allows us to develop a characteristic transfer function that has only one parameter viz. the characteristic switching time and fully predicts the resistive states of this type of memristor.

  12. Carpal tunnel syndrome treatment

    Directory of Open Access Journals (Sweden)

    Emilio Filippucci


    Full Text Available Carpal tunnel syndrome, the most common peripheral neuropathy, results from compression of the median nerve at the wrist, and is a cause of pain, numbness and tingling in the upper extremities and an increasingly recognized cause of work disability. If carpal tunnel syndrome seems likely, conservative management with splinting should be initiated. Moreover, it has suggested that patients reduce activities at home and work that exacerbate symptoms. Pyridoxine and diuretics, since are largely utilised, are no more effective than placebo in relieving the symptoms. Non steroidal anti-inflammatory drugs and orally administered corticosteroids can be effective for short-term management (two to four weeks, but local corticosteroid injection may improve symptoms for a longer period. Injection is especially effective if there is no loss of sensibility or thenar-muscle atrophy and weakness, and if symptoms are intermittent rather than constant. If symptoms are refractory to conservative measures, the option of surgical therapy may be considered.

  13. [Carpal tunnel syndrome treatment]. (United States)

    De Angelis, Rossella; Salaffi, Fausto; Filippucci, Emilio; Grassi, Walter


    Carpal tunnel syndrome, the most common peripheral neuropathy, results from compression of the median nerve at the wrist, and is a cause of pain, numbness and tingling in the upper extremities and an increasingly recognized cause of work disability. If carpal tunnel syndrome seems likely, conservative management with splinting should be initiated. Moreover, it has suggested that patients reduce activities at home and work that exacerbate symptoms. Pyridoxine and diuretics, since are largely utilised, are no more effective than placebo in relieving the symptoms. Non steroidal anti-inflammatory drugs and orally administered corticosteroids can be effective for short-term management (two to four weeks), but local corticosteroid injection may improve symptoms for a longer period. Injection is especially effective if there is no loss of sensibility or thenar-muscle atrophy and weakness, and if symptoms are intermittent rather than constant. If symptoms are refractory to conservative measures, the option of surgical therapy may be considered.

  14. Thiamine triphosphate synthesis in rat brain occurs in mitochondria and is coupled to the respiratory chain. (United States)

    Gangolf, Marjorie; Wins, Pierre; Thiry, Marc; El Moualij, Benaïssa; Bettendorff, Lucien


    In animals, thiamine deficiency leads to specific brain lesions, generally attributed to decreased levels of thiamine diphosphate, an essential cofactor in brain energy metabolism. However, another far less abundant derivative, thiamine triphosphate (ThTP), may also have a neuronal function. Here, we show that in the rat brain, ThTP is essentially present and synthesized in mitochondria. In mitochondrial preparations from brain (but not liver), ThTP can be produced from thiamine diphosphate and P(i). This endergonic process is coupled to the oxidation of succinate or NADH through the respiratory chain but cannot be energized by ATP hydrolysis. ThTP synthesis is strongly inhibited by respiratory chain inhibitors, such as myxothiazol and inhibitors of the H(+) channel of F(0)F(1)-ATPase. It is also impaired by disruption of the mitochondria or by depolarization of the inner membrane (by protonophores or valinomycin), indicating that a proton-motive force (Deltap) is required. Collapsing Deltap after ThTP synthesis causes its rapid disappearance, suggesting that both synthesis and hydrolysis are catalyzed by a reversible H(+)-translocating ThTP synthase. The synthesized ThTP can be released from mitochondria in the presence of external P(i). However, ThTP probably does not accumulate in the cytoplasm in vivo, because it is not detected in the cytosolic fraction obtained from a brain homogenate. Our results show for the first time that a high energy triphosphate compound other than ATP can be produced by a chemiosmotic type of mechanism. This might shed a new light on our understanding of the mechanisms of thiamine deficiency-induced brain lesions.

  15. The beam dump tunnels

    CERN Multimedia

    Patrice Loïez


    In these images workers are digging the tunnels that will be used to dump the counter-circulating beams. Travelling just a fraction under the speed of light, the beams at the LHC will each carry the energy of an aircraft carrier travelling at 12 knots. In order to dispose of these beams safely, a beam dump is used to extract the beam and diffuse it before it collides with a radiation shielded graphite target.

  16. Carpal tunnel release

    DEFF Research Database (Denmark)

    Larsen, Morten Bo; Sørensen, A I; Crone, K L;


    A single-blind, randomized, controlled trial was done to compare the results of carpal tunnel release using classic incision, short incision, or endoscopic technique. In total, 90 consecutive cases were included. Follow-up was 24 weeks. We found a significantly shorter sick leave in the endoscopi...... incision could be found. There were no serious complications in either group. The results indicate that the endoscopic procedure is safe and has the benefit of faster rehabilitation and return to work....

  17. Adenosine triphosphate levels during anaphylactic histamine release in rat mast cells in vitro. Effects of glycolytic and respiratory inhibitors

    DEFF Research Database (Denmark)

    Johansen, Torben


    The adenosine triphosphate (ATP) content of rat mast cells was studied during and after anaphylactic histamine release. The almost identical time course of ATP decrease from mast cells treated with either glycolytic or respiratory inhibitors supports the view that the ATP depletion was largely re...

  18. Regulation of the intersubunit ammonia tunnel in Mycobacterium tuberculosis glutamine-dependent NAD[superscript +] synthetase

    Energy Technology Data Exchange (ETDEWEB)

    Chuenchor, Watchalee; Doukov, Tzanko I.; Resto, Melissa; Chang, Andrew; Gerratana, Barbara (SSRL); (Maryland)


    Glutamine-dependent NAD{sup +} synthetase is an essential enzyme and a validated drug target in Mycobacterium tuberculosis (mtuNadE). It catalyses the ATP-dependent formation of NAD{sup +} from NaAD{sup +} (nicotinic acid-adenine dinucleotide) at the synthetase active site and glutamine hydrolysis at the glutaminase active site. An ammonia tunnel 40 {angstrom} (1 {angstrom} = 0.1 nm) long allows transfer of ammonia from one active site to the other. The enzyme displays stringent kinetic synergism; however, its regulatory mechanism is unclear. In the present paper, we report the structures of the inactive glutaminase C176A variant in an apo form and in three synthetase-ligand complexes with substrates (NaAD{sup +}/ATP), substrate analogue {l_brace}NaAD{sup +}/AMP-CPP (adenosine 5'-[{alpha},{beta}-methylene]triphosphate){r_brace} and intermediate analogues (NaAD{sup +}/AMP/PPi), as well as the structure of wild-type mtuNadE in a product complex (NAD{sup +}/AMP/PPi/glutamate). This series of structures provides snapshots of the ammonia tunnel during the catalytic cycle supported also by kinetics and mutagenesis studies. Three major constriction sites are observed in the tunnel: (i) at the entrance near the glutaminase active site; (ii) in the middle of the tunnel; and (iii) at the end near the synthetase active site. Variation in the number and radius of the tunnel constrictions is apparent in the crystal structures and is related to ligand binding at the synthetase domain. These results provide new insight into the regulation of ammonia transport in the intermolecular tunnel of mtuNadE.

  19. Detection of adenosine triphosphate through polymerization-induced aggregation of actin-conjugated gold/silver nanorods (United States)

    Liao, Yu-Ju; Shiang, Yen-Chun; Chen, Li-Yi; Hsu, Chia-Lun; Huang, Chih-Ching; Chang, Huan-Tsung


    We have developed a simple and selective nanosensor for the optical detection of adenosine triphosphate (ATP) using globular actin-conjugated gold/silver nanorods (G-actin-Au/Ag NRs). By simply mixing G-actin and Au/Ag NRs (length ˜56 nm and diameter ˜12 nm), G-actin-Au/Ag NRs were prepared which were stable in physiological solutions (25 mM Tris-HCl, 150 mM NaCl, 5.0 mM KCl, 3.0 mM MgCl2 and 1.0 mM CaCl2; pH 7.4). Introduction of ATP into the G-actin-Au/Ag NR solutions in the presence of excess G-actin induced the formation of filamentous actin-conjugated Au/Ag NR aggregates through ATP-induced polymerization of G-actin. When compared to G-actin-modified spherical Au nanoparticles having a size of 13 nm or 56 nm, G-actin-Au/Ag NRs provided better sensitivity for ATP, mainly because the longitudinal surface plasmon absorbance of the Au/Ag NR has a more sensitive response to aggregation. This G-actin-Au/Ag NR probe provided high sensitivity (limit of detection 25 nM) for ATP with remarkable selectivity (>10-fold) over other adenine nucleotides (adenosine, adenosine monophosphate and adenosine diphosphate) and nucleoside triphosphates (guanosine triphosphate, cytidine triphosphate and uridine triphosphate). It also allowed the determination of ATP concentrations in plasma samples without conducting tedious sample pretreatments; the only necessary step was simple dilution. Our experimental results are in good agreement with those obtained from a commercial luciferin-luciferase bioluminescence assay. Our simple, sensitive and selective approach appears to have a practical potential for the clinical diagnosis of diseases (e.g. cystic fibrosis) associated with changes in ATP concentrations.

  20. Inelastic tunneling in superconducting junctions

    Energy Technology Data Exchange (ETDEWEB)

    Hlobil, Patrik Christian


    In this dissertation a theoretical formalism of elastic and inelastic tunneling spectroscopy is developed for superconductors. The underlying physical processes behind the different two tunneling channels and their implications for the interpretation of experimental tunneling data are investigated in detail, which can explain the background conductance seen in the cuprate and iron-based superconductors. Further, the properties of the emitted light from a superconducting LED are investigated.

  1. [Bacterial vaginosis]. (United States)

    Romero Herrero, Daniel; Andreu Domingo, Antonia


    Bacterial vaginosis (BV) is the main cause of vaginal dysbacteriosis in the women during the reproductive age. It is an entity in which many studies have focused for years and which is still open for discussion topics. This is due to the diversity of microorganisms that cause it and therefore, its difficult treatment. Bacterial vaginosis is probably the result of vaginal colonization by complex bacterial communities, many of them non-cultivable and with interdependent metabolism where anaerobic populations most likely play an important role in its pathogenesis. The main symptoms are an increase of vaginal discharge and the unpleasant smell of it. It can lead to serious consequences for women, such as an increased risk of contracting sexually transmitted infections including human immunodeficiency virus and upper genital tract and pregnancy complications. Gram stain is the gold standard for microbiological diagnosis of BV, but can also be diagnosed using the Amsel clinical criteria. It should not be considered a sexually transmitted disease but it is highly related to sex. Recurrence is the main problem of medical treatment. Apart from BV, there are other dysbacteriosis less characterized like aerobic vaginitis of which further studies are coming slowly but are achieving more attention and consensus among specialists.

  2. Engineers win award for Swiss tunnel

    CERN Multimedia


    A Derby engineering consultancy has won the Tunnelling Industry Award 2003 for Excellence in Tunnel Design, offered by the British Tunnelling Society, for its work on the LHC in Geneva, Switzerland (1/2 page).

  3. A virtual detector approach to tunnel ionization and tunneling times

    CERN Document Server

    Teeny, Nicolas; Bauke, Heiko


    Tunneling times in atomic ionization is studied theoretically by a virtual detector approach. A virtual detector is a hypothetical device that allows to monitor the wave-function's density with spatial and temporal resolution during the ionization process. With this theoretical approach, it becomes possible to define unique moments when the electron enters and leaves with highest probability the classically forbidden region from first principles and a tunneling time can be specified unambiguously. It is shown that neither the moment when the electron enters the tunneling barrier nor when it leaves the tunneling barrier coincide with the moment when the external electric field reaches its maximum. Under the tunneling barrier as well as at the exit the electron has a nonzero velocity in electric field direction. This nonzero exit velocity has to be incorporated when the free motion of the electron is modeled by classical equations of motion.

  4. Tunnel face stability and ground settlement in pressurized shield tunnelling

    Institute of Scientific and Technical Information of China (English)

    苏艺; 汪国锋; 周庆宏


    An analysis of the stability of large-diameter circular tunnels and ground settlement during tunnelling by a pressurized shield was presented. An innovative three-dimensional translational multi-block failure mechanism was proposed to determine the face support pressure of large-shield tunnelling. Compared with the currently available mechanisms, the proposed mechanism has two unique features: (1) the supporting pressure applied to the tunnel face is assumed to have a non-uniform rather than uniform distribution, and (2) the method takes into account the entire circular excavation face instead of merely an inscribed ellipse. Based on the discrete element method, a numerical simulation of the Shanghai Yangtze River Tunnel was carried out using the Particle Flow Code in two dimensions. The immediate ground movement during excavation, as well as the behaviour of the excavation face, the shield movement, and the excavated area, was considered before modelling the excavation process.

  5. Tunneling Ionization of Diatomic Molecules

    DEFF Research Database (Denmark)

    Svensmark, Jens Søren Sieg


    barriers, an ability classical particles do not possess. Tunnelling is a fundamental quantum mechanical process, a process that is distinctly non-classical, so solving this tunnelling problem is not only relevant for molecular physics, but also for quantum theory in general. In this dissertation the theory...

  6. Tunneling magnetic force microscopy (United States)

    Burke, Edward R.; Gomez, Romel D.; Adly, Amr A.; Mayergoyz, Isaak D.


    We have developed a powerful new tool for studying the magnetic patterns on magnetic recording media. This was accomplished by modifying a conventional scanning tunneling microscope. The fine-wire probe that is used to image surface topography was replaced with a flexible magnetic probe. Images obtained with these probes reveal both the surface topography and the magnetic structure. We have made a thorough theoretical analysis of the interaction between the probe and the magnetic fields emanating from a typical recorded surface. Quantitative data about the constituent magnetic fields can then be obtained. We have employed these techniques in studies of two of the most important issues of magnetic record: data overwrite and maximizing data-density. These studies have shown: (1) overwritten data can be retrieved under certain conditions; and (2) improvements in data-density will require new magnetic materials. In the course of these studies we have developed new techniques to analyze magnetic fields of recorded media. These studies are both theoretical and experimental and combined with the use of our magnetic force scanning tunneling microscope should lead to further breakthroughs in the field of magnetic recording.

  7. Tunnel electroresistance through organic ferroelectrics (United States)

    Tian, B. B.; Wang, J. L.; Fusil, S.; Liu, Y.; Zhao, X. L.; Sun, S.; Shen, H.; Lin, T.; Sun, J. L.; Duan, C. G.; Bibes, M.; Barthélémy, A.; Dkhil, B.; Garcia, V.; Meng, X. J.; Chu, J. H.


    Organic electronics is emerging for large-area applications such as photovoltaic cells, rollable displays or electronic paper. Its future development and integration will require a simple, low-power organic memory, that can be written, erased and readout electrically. Here we demonstrate a non-volatile memory in which the ferroelectric polarisation state of an organic tunnel barrier encodes the stored information and sets the readout tunnel current. We use high-sensitivity piezoresponse force microscopy to show that films as thin as one or two layers of ferroelectric poly(vinylidene fluoride) remain switchable with low voltages. Submicron junctions based on these films display tunnel electroresistance reaching 1,000% at room temperature that is driven by ferroelectric switching and explained by electrostatic effects in a direct tunnelling regime. Our findings provide a path to develop low-cost, large-scale arrays of organic ferroelectric tunnel junctions on silicon or flexible substrates.

  8. Carpal tunnel syndrome and acromegaly. (United States)

    Baum, H; Lüdecke, D K; Herrmann, H D


    50 patients with acromegaly and carpal tunnel syndrome have been examined electrophysiologically before and after transnasal operation of the pituitary adenoma. 32 of the 50 patients (64%) had symptoms of carpal tunnel syndrome. 13 of them had neurological deficits. 28 of the examined patients had pathological neurographical findings only. About 1 week post-operatively DL was decreased in 43%; in 10 out of 13 patients with neurological deficits DL decreased. GH was normalized in 80% and reduced to 5-10 micrograms/l in a further 10%. The investigation did not show whether the carpal tunnel syndrome only depended on a GH increase or on other factors also such as e.g., on the duration of symptoms or tissue changes. None of the patients had the transversal carpal ligament operated on. The coincidence between acromegaly and carpal tunnel syndrome was 64%. In 3 cases the carpal tunnel syndrome was the leading sign to the diagnosis of acromegaly.

  9. Electroacupuncture improves neuropathic pain Adenosine,adenosine 5'-triphosphate disodium and their receptors perhaps change simultaneously

    Institute of Scientific and Technical Information of China (English)

    Wen Ren; Wenzhan Tu; Songhe Jiang; Ruidong Cheng; Yaping Du


    Applying a stimulating current to acupoints through acupuncture needles-known as electroacupuncture-has the potential to produce analgesic effects in human subjects and experimental animals.When acupuncture was applied in a rat model,adenosine 5'-triphosphate disodium in the extracellular space was broken down into adenosine,which in turn inhibited pain transmission by means of an adenosine A1 receptor-dependent process.Direct injection of an adenosine A1 receptor agonist enhanced the analgesic effect of acupuncture.The analgesic effect of acupuncture appears to be mediated by activation of A1 receptors located on ascending nerves.In neuropathic pain,there is upregulation of P2X purinoceptor 3(P2X3)receptor expression in dorsal root ganglion neurons.Conversely,the onset of mechanical hyperalgesia was diminished and established hyperalgesia was significantly reversed when P2X3 receptor expression was downregulated.The pathways upon which electroacupuncture appear to act are interwoven with pain pathways,and electroacupuncture stimuli converge with impulses originating from painful areas.Electroacupuncture may act via purinergic A1 and P2X3 receptors simultaneously to induce an analgesic effect on neuropathic pain.

  10. Trypanosoma brucei brucei: effects of ferrous iron and heme on ecto-nucleoside triphosphate diphosphohydrolase activity. (United States)

    Leite, Milane S; Thomaz, Rachel; Oliveira, José Henrique M; Oliveira, Pedro L; Meyer-Fernandes, José Roberto


    Trypanosoma brucei brucei is the causative agent of animal African trypanosomiasis, also called nagana. Procyclic vector form resides in the midgut of the tsetse fly, which feeds exclusively on blood. Hemoglobin digestion occurs in the midgut resulting in an intense release of free heme. In the present study we show that the magnesium-dependent ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase) activity of procyclic T. brucei brucei is inhibited by ferrous iron and heme. The inhibition of E-NTPDase activity by ferrous iron, but not by heme, was prevented by pre-incubation of cells with catalase. However, antioxidants that permeate cells, such as PEG-catalase and N-acetyl-cysteine prevented the inhibition of E-NTPDase by heme. Ferrous iron was able to induce an increase in lipid peroxidation, while heme did not. Therefore, both ferrous iron and heme can inhibit E-NTPDase activity of T. brucei brucei by means of formation of reactive oxygen species, but apparently acting through distinct mechanisms.


    Institute of Scientific and Technical Information of China (English)

    杨军; 李吉平; 钱敏飞; 徐秀玲; 王家东; 丁大连


    Objective To study effects of adenosine 5'-triphosphate (ATP) on cochlear function of guinea pig. Methods After perfusion of ATP into perilymphatic spaces of the guinea pig cochlea, summating potential (SP), cochlear microphonic (CM) , auditory nerve compound action potential ( CAP ) , distortion product otoacoustic emission (DPOAE) and auditory brainstem response (ABR) were measured. Results The results showed concentration-dependent effect of ATP on the response alterations of bioelectric activity in cochlea. Administration of lmmol/L ATP caused an increase both in the amplitude of the SP and in the threshold of ABR, a decrease in amplitude of the CAP and DPOAE. In addition, response alterations of the CAP and DPOAE showed in an intensity- and frequency-dependent manner, respectively. At levels of 20 -70dB nHL sound intensity, lmmol/L ATP caused a significant decrease in the CAP amplitude, while at moderate and high frequency ranges of 2 -8kHz it reduced DPOAE amplitude significantly. 330μmol/L ATP also increased the threshold of ABR. Conclusion ATP through perilymphatic perfusion could inhibit cochlear function of guinea pig.

  12. In vivo effects of adenosine 5´-triphosphate on rat preneoplastic liver

    Directory of Open Access Journals (Sweden)

    Ana V. Frontini


    Full Text Available The utilization of adenosine 5´-triphosphate (ATP infusions to inhibit the growth of some human and animals tumors was based on the anticancer activity observed in in vitro and in vivo experiments, but contradictory results make the use of ATP in clinical practice rather controversial. Moreover, there is no literature regarding the use of ATP infusions to treat hepatocarcinomas. The purpose of this study was to investigate whether ATP prevents in vivo oncogenesis in very-early-stage cancer cells in a well characterized two-stage model of hepatocarcinogenesis in the rat. As we could not preclude the possible effect due to the intrinsic properties of adenosine, a known tumorigenic product of ATP hydrolysis, the effect of the administration of adenosine was also studied. Animals were divided in groups: rats submitted to the two stage preneoplasia initiation/promotion model of hepatocarcinogenesis, rats treated with intraperitoneal ATP or adenosine during the two phases of the model and appropriate control groups. The number and volume of preneoplastic foci per liver identified by the expression of glutathione S-transferase placental type and the number of proliferating nuclear antigen positive cells significantly increased in ATP and adenosine treated groups. Taken together, these results indicate that in this preneoplastic liver model, ATP as well as adenosine disturb the balance between apoptosis and proliferation contributing to malignant transformation.

  13. Five putative nucleoside triphosphate diphosphohydrolase genes are expressed in Trichomonas vaginalis. (United States)

    Frasson, Amanda Piccoli; Dos Santos, Odelta; Meirelles, Lúcia Collares; Macedo, Alexandre José; Tasca, Tiana


    Trichomonas vaginalis is a protozoan that parasitizes the human urogenital tract causing trichomoniasis, the most common non-viral sexually transmitted disease. The parasite has unique genomic characteristics such as a large genome size and expanded gene families. Ectonucleoside triphosphate diphosphohydrolase (E-NTPDase) is an enzyme responsible for hydrolyzing nucleoside tri- and diphosphates and has already been biochemically characterized in T. vaginalis. Considering the important role of this enzyme in the production of extracellular adenosine for parasite uptake, we evaluated the gene expression of five putative NTPDases in T. vaginalis. We showed that all five putative TvNTPDase genes (TvNTPDase1-5) were expressed by both fresh clinical and long-term grown isolates. The amino acid alignment predicted the presence of the five crucial apyrase conserved regions, transmembrane domains, signal peptides, phosphorylation and catalytic sites. Moreover, a phylogenetic analysis showed that TvNTPDase sequences make up a clade with NTPDases intracellularly located. Biochemical NTPDase activity (ATP and ADP hydrolysis) is responsive to the serum-restrictive conditions and the gene expression of TvNTPDases was mostly increased, mainly TvNTPDase2 and TvNTPDase4, although there was not a clear pattern of expression among them. In summary, the present report demonstrates the gene expression patterns of predicted NTPDases in T. vaginalis.

  14. Adenosine triphosphate bioluminescence analysis for rapid screening of microbial contamination in non-sterile pharmaceutical samples. (United States)

    Jimenez, Luis


    An Adenosine Triphosphate (ATP) bioluminescence system was compared and validated against standard methods for rapid microbiological monitoring of several non-sterile pharmaceutical formulations such as creams, tablets, and capsules. Results obtained using 1%, 2.5%, and 10% of product suspensions indicated that most samples that did not contain non-microbial ATP neither inhibited the bioluminescence reaction nor did something else. Ten percent product suspensions were inoculated with different concentrations of Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, Candida albicans, and Aspergillus niger. Samples were incubated for 24-120 h at 35 degrees C with shaking. Results indicated a strong inhibitory effect of microbial growth, as no microorganisms were detected by using the ATP bioluminescence assay. However, when 1% and 2.5% product suspensions were spiked with the same microorganisms, positive detection was confirmed. After incubation, all microorganisms were detected by the bioluminescence system within 24-72 h. All positive samples were confirmed by using standard plating media. However, to optimize detection of all microorganisms, different enrichment media were developed.

  15. An efficient extraction method for quantitation of adenosine triphosphate in mammalian tissues and cells. (United States)

    Chida, Junji; Yamane, Kazuhiko; Takei, Tunetomo; Kido, Hiroshi


    Firefly bioluminescence is widely used in the measurement of adenosine 5'-triphosphate (ATP) levels in biological materials. For such assays in tissues and cells, ATP must be extracted away from protein in the initial step and extraction efficacy is the main determinant of the assay accuracy. Extraction reagents recommended in the commercially available ATP assay kits are chaotropic reagents, trichloroacetic acid (TCA), perchloric acid (PCA), and ethylene glycol (EG), which extract nucleotides through protein precipitation and/or nucleotidase inactivation. We found that these reagents are particularly useful for measuring ATP levels in materials with relatively low protein concentrations such as blood cells, cultured cells, and bacteria. However, these methods are not suitable for ATP extraction from tissues with high protein concentrations, because some ATP may be co-precipitated with the insolubilized protein during homogenization and extraction, and it could also be precipitated by neutralization in the acid extracts. Here we found that a phenol-based extraction method markedly increased the ATP and other nucleotides extracted from tissues. In addition, phenol extraction does not require neutralization before the luciferin-luciferase assay step. ATP levels analyzed by luciferase assay in various tissues extracted by Tris-EDTA-saturated phenol (phenol-TE) were over 17.8-fold higher than those extracted by TCA and over 550-fold higher than those in EG extracts. Here we report a simple, rapid, and reliable phenol-TE extraction procedure for ATP measurement in tissues and cells by luciferase assay.

  16. Light scattering change precedes loss of cerebral adenosine triphosphate in a rat global ischemic brain model. (United States)

    Kawauchi, Satoko; Sato, Shunichi; Ooigawa, Hidetoshi; Nawashiro, Hiroshi; Ishihara, Miya; Kikuchi, Makoto


    Measurement of intrinsic optical signals (IOSs) is an attractive technique for monitoring tissue viability in brains since it enables noninvasive, real-time monitoring of morphological characteristics as well as physiological and biochemical characteristics of tissue. We previously showed that light scattering signals reflecting cellular morphological characteristics were closely related to the IOSs associated with the redox states of cytochrome c oxidase in the mitochondrial respiratory chain. In the present study, we examined the relationship between light scattering and energy metabolism. Light scattering signals were transcranially measured in rat brains after oxygen and glucose deprivation, and the results were compared with concentrations of cerebral adenosine triphosphate (ATP) measured by luciferin-luciferase bioluminescence assay. Electrophysiological signal was also recorded simultaneously. After starting saline infusion, EEG activity ceased at 108+/-17s, even after which both the light scattering signal and ATP concentration remained at initial levels. However, light scattering started to change in three phases at 236+/-15s and then cerebral ATP concentration started to decrease at about 260s. ATP concentration significantly decreased during the triphasic scattering change, indicating that the start of scattering change preceded the loss of cerebral ATP. The mean time difference between the start of triphasic scattering change and the onset of ATP loss was about 24s in the present model. DC potential measurement showed that the triphasic scattering change was associated with anoxic depolarization. These findings suggest that light scattering signal can be used as an indicator of loss of tissue viability in brains.

  17. A novel conductometric biosensor based on hexokinase for determination of adenosine triphosphate. (United States)

    Kucherenko, I S; Kucherenko, D Yu; Soldatkin, O O; Lagarde, F; Dzyadevych, S V; Soldatkin, A P


    The paper presents a simple and inexpensive reusable biosensor for determination of the concentration of adenosine-5'-triphosphate (ATP) in aqueous samples. The biosensor is based on a conductometric transducer which contains two pairs of gold interdigitated electrodes. An enzyme hexokinase was immobilized onto one pair of electrodes, and bovine serum albumin-onto another pair (thus, a differential mode of measurement was used). Conditions of hexokinase immobilization on the transducer by cross-linking via glutaraldehyde were optimized. Influence of experimental conditions (concentration of magnesium ions, ionic strength and concentration of the working buffer) on the biosensor work was studied. The reproducibility of biosensor responses and operational stability of the biosensor were checked during one week. Dry storage at -18 °C was shown to be the best conditions to store the biosensor. The biosensor was successfully applied for measurements of ATP concentration in pharmaceutical samples. The proposed biosensor may be used in future for determination of ATP and/or glucose in water samples.

  18. A Graphene and Aptamer Based Liquid Gated FET-Like Electrochemical Biosensor to Detect Adenosine Triphosphate. (United States)

    Mukherjee, Souvik; Meshik, Xenia; Choi, Min; Farid, Sidra; Datta, Debopam; Lan, Yi; Poduri, Shripriya; Sarkar, Ketaki; Baterdene, Undarmaa; Huang, Ching-En; Wang, Yung Yu; Burke, Peter; Dutta, Mitra; Stroscio, Michael A


    Here we report successful demonstration of a FET-like electrochemical nano-biosensor to accurately detect ultralow concentrations of adenosine triphosphate. As a 2D material, graphene is a promising candidate due to its large surface area, biocompatibility, and demonstrated surface binding chemistries and has been employed as the conducting channel. A short 20-base DNA aptamer is used as the sensing element to ensure that the interaction between the analyte and the aptamer occurs within the Debye length of the electrolyte (PBS). Significant increase in the drain current with progressive addition of ATP is observed whereas for control experiments, no distinct change in the drain current occurs. The sensor is found to be highly sensitive in the nanomolar (nM) to micromolar ( μM) range with a high sensitivity of 2.55 μA (mM) (-1), a detection limit as low as 10 pM, and it has potential application in medical and biological settings to detect low traces of ATP. This simplistic design strategy can be further extended to efficiently detect a broad range of other target analytes.

  19. [Nucleoside-5'-triphosphate hydrolysis in the liver and kidney of rats with chronic alloxan diabetes]. (United States)

    Rusina, I M; Makarchikov, A F; Makar, E A; Kubyshin, V L


    Activity and some properties of a soluble enzyme hydrolyzing nucleoside-5'-triphosphates were studied in the liver and kidney of normal and diabetic rats. The enzyme activity was shown to be reduced by 34% (p < 0.01) in the liver extracts of diabetic animals, while no difference was observed in the kidney. When ITP was used as substrate, the apparent Michaelis constant of the enzyme was significantly lower in the liver of controls as compared to experimental rats (32.3 +/- 1.3 microM and 54.3 +/- 1.0 microM, respectively, p < 0.01). The KM values of the enzyme in the kidney were not distinguishable in both groups. NTPase exhibits maximal activity at pH 7.0 and has a broad substrate specificity with respect to different nucleoside-5'-tri- and diphosphates. Molecular mass of the enzyme was estimated by gel filtration to be 63.7 +/- 0.9 kD.

  20. Age-associated repression of type 1 inositol 1, 4, 5-triphosphate receptor impairs muscle regeneration (United States)

    Lee, Bora; Lee, Seung-Min; Bahn, Young Jae; Lee, Kwang-Pyo; Kang, Moonkyung; Kim, Yeon-Soo; Woo, Sun-Hee; Lim, Jae-Young; Kim, Eunhee; Kwon, Ki-Sun


    Skeletal muscle mass and power decrease with age, leading to impairment of mobility and metabolism in the elderly. Ca2+ signaling is crucial for myoblast differentiation as well as muscle contraction through activation of transcription factors and Ca2+-dependent kinases and phosphatases. Ca2+ channels, such as dihydropyridine receptor (DHPR), two-pore channel (TPC) and inositol 1,4,5-triphosphate receptor (ITPR), function to maintain Ca2+ homeostasis in myoblasts. Here, we observed a significant decrease in expression of type 1 IP3 receptor (ITPR1), but not types 2 and 3, in aged mice skeletal muscle and isolated myoblasts, compared with those of young mice. ITPR1 knockdown using shRNA-expressing viruses in C2C12 myoblasts and tibialis anterior muscle of mice inhibited myotube formation and muscle regeneration after injury, respectively, a typical phenotype of aged muscle. This aging phenotype was associated with repression of muscle-specific genes and activation of the epidermal growth factor receptor (EGFR)-Ras-extracellular signal-regulated kinase (ERK) pathway. ERK inhibition by U0126 not only induced recovery of myotube formation in old myoblasts but also facilitated muscle regeneration after injury in aged muscle. The conserved decline in ITPR1 expression in aged human skeletal muscle suggests utility as a potential therapeutic target for sarcopenia, which can be treated using ERK inhibition strategies. PMID:27658230

  1. Ionic interaction of amiloride and uridine 5'-triphosphate in nebulizer solutions. (United States)

    Pettis, Ronald J; Knowles, Michael R; Olivier, Kenneth N; Kazantseva, Masha; Hickey, Anthony J


    Combination therapy using nebulized amiloride hydrochloride and uridine-5'-triphosphate (UTP) trisodium salt aerosols has been investigated for the treatment of cystic fibrosis (CF). Amiloride in aqueous solution precipitates in the presence of UTP, reducing drug concentrations. Interactions between these drugs and NaCl in solution were studied using phase-solubility techniques monitored by UV spectrophotometry. Elemental analyses were employed for precipitate characterization. Amiloride solubility was reduced by more than 85% in saline. Amiloride solubility decreased with increasing UTP concentration, resulting in formation of a precipitated complex. The theoretical molar ratio of complexes range from 1-3 amiloride:1 UTP. At most concentrations only 3 amiloride:1 UTP complex was observed in precipitate. This is a reflection of low Ksp for the 3:1 complex of 2.92 x 10(-11) M4 compared with 2.09 x 10(-4) M2 for amiloride alone. Equilibration over excess bulk solid resulted in higher solubility estimates and different phase solubility diagrams than solubility studies utilizing precipitation technique. This may be explained by the absence of amiloride in the solid state and its impact on complex equilibria with UTP. The solubility suppressing effects of UTP and saline were largely additive. A number of ionic interactions increase complex solubility profile of amiloride hydrochloride in the presence of UTP and NaCl.

  2. The roles of initiation factor 2 and guanosine triphosphate in initiation of protein synthesis (United States)

    Antoun, Ayman; Pavlov, Michael Y.; Andersson, Kerstin; Tenson, Tanel; Ehrenberg, Måns


    The role of IF2 from Escherichia coli was studied in vitro using a system for protein synthesis with purified components. Stopped flow experiments with light scattering show that IF2 in complex with guanosine triphosphate (GTP) or a non-cleavable GTP analogue (GDPNP), but not with guanosine diphosphate (GDP), promotes fast association of ribosomal subunits during initiation. Biochemical experiments show that IF2 promotes fast formation of the first peptide bond in the presence of GTP, but not GDPNP or GDP, and that IF2–GDPNP binds strongly to post-initiation ribosomes. We conclude that the GTP form of IF2 accelerates formation of the 70S ribosome from subunits and that GTP hydrolysis accelerates release of IF2 from the 70S ribosome. The results of a recent report, suggesting that GTP and GDP promote initiation equally fast, have been addressed. Our data, indicating that eIF5B and IF2 have similar functions, are used to rationalize the phenotypes of GTPase-deficient mutants of eIF5B and IF2. PMID:14532131

  3. Visual and Plasmon Resonance Absorption Sensor for Adenosine Triphosphate Based on the High Affinity between Phosphate and Zr(IV) (United States)

    Qi, Wenjing; Liu, Zhongyuan; Zhang, Wei; Halawa, Mohamed Ibrahim; Xu, Guobao


    Zr(IV) can form phosphate and Zr(IV) (–PO32−–Zr4+–) complex owing to the high affinity between Zr(IV) with phosphate. Zr(IV) can induce the aggregation of gold nanoparticles (AuNPs), while adenosine triphosphate(ATP) can prevent Zr(IV)-induced aggregation of AuNPs. Herein, a visual and plasmon resonance absorption (PRA)sensor for ATP have been developed using AuNPs based on the high affinity between Zr(IV)with ATP. AuNPs get aggregated in the presence of certain concentrations of Zr(IV). After the addition of ATP, ATP reacts with Zr(IV) and prevents AuNPs from aggregation, enabling the detection of ATP. Because of the fast interaction of ATP with Zr(IV), ATP can be detected with a detection limit of 0.5 μM within 2 min by the naked eye. Moreover, ATP can be detected by the PRA technique with higher sensitivity. The A520nm/A650nm values in PRA spectra increase linearly with the concentrations of ATP from 0.1 μM to 15 μM (r = 0.9945) with a detection limit of 28 nM. The proposed visual and PRA sensor exhibit good selectivity against adenosine, adenosine monophosphate, guanosine triphosphate, cytidine triphosphate and uridine triphosphate. The recoveries for the analysis of ATP in synthetic samples range from 95.3% to 102.0%. Therefore, the proposed novel sensor for ATP is promising for real-time or on-site detection of ATP. PMID:27754349

  4. Visual and Plasmon Resonance Absorption Sensor for Adenosine Triphosphate Based on the High Affinity between Phosphate and Zr(IV

    Directory of Open Access Journals (Sweden)

    Wenjing Qi


    Full Text Available Zr(IV can form phosphate and Zr(IV (–PO32−–Zr4+– complex owing to the high affinity between Zr(IV with phosphate. Zr(IV can induce the aggregation of gold nanoparticles (AuNPs, while adenosine triphosphate(ATP can prevent Zr(IV-induced aggregation of AuNPs. Herein, a visual and plasmon resonance absorption (PRAsensor for ATP have been developed using AuNPs based on the high affinity between Zr(IVwith ATP. AuNPs get aggregated in the presence of certain concentrations of Zr(IV. After the addition of ATP, ATP reacts with Zr(IV and prevents AuNPs from aggregation, enabling the detection of ATP. Because of the fast interaction of ATP with Zr(IV, ATP can be detected with a detection limit of 0.5 μM within 2 min by the naked eye. Moreover, ATP can be detected by the PRA technique with higher sensitivity. The A520nm/A650nm values in PRA spectra increase linearly with the concentrations of ATP from 0.1 μM to 15 μM (r = 0.9945 with a detection limit of 28 nM. The proposed visual and PRA sensor exhibit good selectivity against adenosine, adenosine monophosphate, guanosine triphosphate, cytidine triphosphate and uridine triphosphate. The recoveries for the analysis of ATP in synthetic samples range from 95.3% to 102.0%. Therefore, the proposed novel sensor for ATP is promising for real-time or on-site detection of ATP.

  5. Thiamin diphosphate in biological chemistry: new aspects of thiamin metabolism, especially triphosphate derivatives acting other than as cofactors. (United States)

    Bettendorff, Lucien; Wins, Pierre


    Prokaryotes, yeasts and plants synthesize thiamin (vitamin B1) via complex pathways. Animal cells capture the vitamin through specific high-affinity transporters essential for internal thiamin homeostasis. Inside the cells, thiamin is phosphorylated to higher phosphate derivatives. Thiamin diphosphate (ThDP) is the best-known thiamin compound because of its role as an enzymatic cofactor. However, in addition to ThDP, at least three other thiamin phosphates occur naturally in most cells: thiamin monophosphate, thiamin triphosphate (ThTP) and the recently discovered adenosine thiamin triphosphate. It has been suggested that ThTP has a specific neurophysiological role, but recent data favor a much more basic metabolic function. During amino acid starvation, Escherichia coli accumulate ThTP, possibly acting as a signal involved in the adaptation of the bacteria to changing nutritional conditions. In animal cells, ThTP can phosphorylate some proteins, but the physiological significance of this mechanism remains unknown. Adenosine thiamin triphosphate, recently discovered in E. coli, accumulates during carbon starvation and might act as an alarmone. Among the proteins involved in thiamin metabolism, thiamin transporters, thiamin pyrophosphokinase and a soluble 25-kDa thiamin triphosphatase have been characterized at the molecular level, in contrast to thiamin mono- and diphosphatases whose specificities remain to be proven. A soluble enzyme catalyzing the synthesis of adenosine thiamin triphosphate from ThDP and ADP or ATP has been partially characterized in E. coli, but the mechanism of ThTP synthesis remains elusive. The data reviewed here illustrate the complexity of thiamin biochemistry, which is not restricted to the cofactor role of ThDP.

  6. Frequency driven inversion of tunnel magnetoimpedance and observation of positive tunnel magnetocapacitance in magnetic tunnel junctions (United States)

    Parui, Subir; Ribeiro, Mário; Atxabal, Ainhoa; Bedoya-Pinto, Amilcar; Sun, Xiangnan; Llopis, Roger; Casanova, Fèlix; Hueso, Luis E.


    The relevance for modern computation of non-volatile high-frequency memories makes ac-transport measurements of magnetic tunnel junctions (MTJs) crucial for exploring this regime. Here, we demonstrate a frequency-mediated effect in which the tunnel magnetoimpedance reverses its sign in a classical Co/Al2O3/NiFe MTJ, whereas we only observe a gradual decrease in the tunnel magnetophase. Such effects are explained by the capacitive coupling of a parallel resistor and capacitor in the equivalent circuit model of the MTJ. Furthermore, we report a positive tunnel magnetocapacitance effect, suggesting the presence of a spin-capacitance at the two ferromagnet/tunnel-barrier interfaces. Our results are important for understanding spin transport phenomena at the high frequency regime in which the spin-polarized charge accumulation due to spin-dependent penetration depth at the two interfaces plays a crucial role.

  7. Instrument detects bacterial life forms (United States)

    Plakas, C.


    Instrument assays enzymatic bioluminescent reaction that occurs when adenosine triphosphate /ATP/ combines with lucifrase and luciferin. Module assembly minimizes need for hardware associated with reaction fluid and waste transfer. System is applicable in marine biology and aerospace and medical fields.

  8. Bacterial hydrodynamics

    CERN Document Server

    Lauga, Eric


    Bacteria predate plants and animals by billions of years. Today, they are the world's smallest cells yet they represent the bulk of the world's biomass, and the main reservoir of nutrients for higher organisms. Most bacteria can move on their own, and the majority of motile bacteria are able to swim in viscous fluids using slender helical appendages called flagella. Low-Reynolds-number hydrodynamics is at the heart of the ability of flagella to generate propulsion at the micron scale. In fact, fluid dynamic forces impact many aspects of bacteriology, ranging from the ability of cells to reorient and search their surroundings to their interactions within mechanically and chemically-complex environments. Using hydrodynamics as an organizing framework, we review the biomechanics of bacterial motility and look ahead to future challenges.

  9. Tunnel fire testing and modeling the Morgex North tunnel experiment

    CERN Document Server

    Borghetti, Fabio; Gandini, Paolo; Frassoldati, Alessio; Tavelli, Silvia


    This book aims to cast light on all aspects of tunnel fires, based on experimental activities and theoretical and computational fluid dynamics (CFD) analyses. In particular, the authors describe a transient full-scale fire test (~15 MW), explaining how they designed and performed the experimental activity inside the Morgex North tunnel in Italy. The entire organization of the experiment is described, from preliminary evaluations to the solutions found for management of operational difficulties and safety issues. This fire test allowed the collection of different measurements (temperature, air velocity, smoke composition, pollutant species) useful for validating and improving CFD codes and for testing the real behavior of the tunnel and its safety systems during a diesel oil fire with a significant heat release rate. Finally, the fire dynamics are compared with empirical correlations, CFD simulations, and literature measurements obtained in other similar tunnel fire tests. This book will be of interest to all ...

  10. Dynamical tunneling theory and experiment

    CERN Document Server

    Keshavamurthy, Srihari


    A prominent aspect of quantum theory, tunneling arises in a variety of contexts across several fields of study, including nuclear, atomic, molecular, and optical physics and has led to technologically relevant applications in mesoscopic science. Exploring mechanisms and consequences, Dynamical Tunneling: Theory and Experiment presents the work of international experts who discuss the considerable progress that has been achieved in this arena in the past two decades.Highlights in this volume include:A historical introduction and overview of dynamical tunneling, with case histories ranging from

  11. Quantum Tunneling and Complex Trajectories (United States)

    Meynig, Max; Haggard, Hal


    In general, the semiclassical approximation of quantum mechanical tunneling fails to treat tunneling through barriers if real initial conditions and trajectories are used. By analytically continuing classical dynamics to the complex plane the problems encountered in the approximation can be resolved. While, the complex methods discussed here have been previously explored, no one has exhibited an analytically solvable case. The essential features of the complex method will be discussed in the context of a novel, analytically solvable problem. These methods could be useful in quantum gravity, with applications to the tunneling of spacetime geometries.

  12. Tunnel Cost-Estimating Methods. (United States)



  13. Tunneling of atoms, nuclei and molecules

    CERN Document Server

    Bertulani, C A


    This is a brief review of few relevant topics on tunneling of composite particles and how the coupling to intrinsic and external degrees of freedom affects tunneling probabilities. I discuss the phenomena of resonant tunneling, different barriers seen by subsystems, damping of resonant tunneling by level bunching and continuum effects due to particle dissociation.

  14. Bacterial vaginosis -- aftercare (United States)

    ... this page: // Bacterial vaginosis - aftercare To use the sharing features on this ... to back after you use the bathroom. Preventing Bacterial Vaginosis You can help prevent bacterial vaginosis by: Not ...

  15. Pregnancy Complications: Bacterial Vaginosis (United States)

    ... Complications & Loss > Pregnancy complications > Bacterial vaginosis and pregnancy Bacterial vaginosis and pregnancy E-mail to a friend Please ... this page It's been added to your dashboard . Bacterial vaginosis (also called BV or vaginitis) is an infection ...

  16. Coxsackievirus cloverleaf RNA containing a 5' triphosphate triggers an antiviral response via RIG-I activation.

    Directory of Open Access Journals (Sweden)

    Qian Feng

    Full Text Available Upon viral infections, pattern recognition receptors (PRRs recognize pathogen-associated molecular patterns (PAMPs and stimulate an antiviral state associated with the production of type I interferons (IFNs and inflammatory markers. Type I IFNs play crucial roles in innate antiviral responses by inducing expression of interferon-stimulated genes and by activating components of the adaptive immune system. Although pegylated IFNs have been used to treat hepatitis B and C virus infections for decades, they exert substantial side effects that limit their use. Current efforts are directed toward the use of PRR agonists as an alternative approach to elicit host antiviral responses in a manner similar to that achieved in a natural infection. RIG-I is a cytosolic PRR that recognizes 5' triphosphate (5'ppp-containing RNA ligands. Due to its ubiquitous expression profile, induction of the RIG-I pathway provides a promising platform for the development of novel antiviral agents and vaccine adjuvants. In this study, we investigated whether structured RNA elements in the genome of coxsackievirus B3 (CVB3, a picornavirus that is recognized by MDA5 during infection, could activate RIG-I when supplied with 5'ppp. We show here that a 5'ppp-containing cloverleaf (CL RNA structure is a potent RIG-I inducer that elicits an extensive antiviral response that includes induction of classical interferon-stimulated genes, as well as type III IFNs and proinflammatory cytokines and chemokines. In addition, we show that prophylactic treatment with CVB3 CL provides protection against various viral infections including dengue virus, vesicular stomatitis virus and enterovirus 71, demonstrating the antiviral efficacy of this RNA ligand.

  17. CaMKII Regulation of Cardiac Ryanodine Receptors and Inositol Triphosphate Receptors

    Directory of Open Access Journals (Sweden)

    Emmanuel eCamors


    Full Text Available Ryanodine receptors (RyRs and inositol triphosphate receptors (InsP3Rs are structurally related intracellular calcium release channels that participate in multiple primary or secondary amplified Ca2+ signals, triggering muscle contraction and oscillatory Ca2+ waves, or activating transcription factors. In the heart, RyRs play an indisputable role in the process of excitation-contraction coupling as the main pathway for Ca2+ release from sarcoplasmic reticulum (SR, and a less prominent role in the process of excitation-transcription coupling. Conversely, InsP3Rs are believed to contribute in subtle ways, only, to contraction of the heart, and in more important ways to regulation of transcription factors. Because uncontrolled activity of either RyRs or InsP3Rs may elicit life-threatening arrhythmogenic and/or remodeling Ca2+ signals, regulation of their activity is of paramount importance for normal cardiac function. Due to their structural similarity, many regulatory factors, accessory proteins, and posttranslational processes are equivalent for RyRs and InsP3Rs. Here we discuss regulation of RyRs and InsP3Rs by CaMKII phosphorylation, but touch on other kinases whenever appropriate. CaMKII is emerging as a powerful modulator of RyR and InsP3R activity but interestingly, some of the complexities and controversies surrounding phosphorylation of RyRs also apply to InsP3Rs, and a clear-cut effect of CaMKII on either channel eludes investigators for now. Nevertheless, some effects of CaMKII on global cellular activity, such as SR Ca2+ leak or force-frequency potentiation, appear clear now, and this constrains the limits of the controversies and permits a more tractable approach to elucidate the effects of phosphorylation at the single channel level.

  18. Adsorption of nucleotides on biomimetic apatite: The case of adenosine 5⿲ triphosphate (ATP) (United States)

    Hammami, Khaled; El-Feki, Hafed; Marsan, Olivier; Drouet, Christophe


    ATP is a well-known energy supplier in cells. The idea to associate ATP to pharmaceutical formulations/biotechnological devices to promote cells activity by potentially modulating their microenvironment thus appears as an appealing novel approach. Since biomimetic nanocrystalline apatites have shown great promise for biomedical applications (bone regeneration, cells diagnostics/therapeutics, ⿦), thanks to a high surface reactivity and an intrinsically high biocompatibility, the present contribution was aimed at exploring ATP/apatite interactions. ATP adsorption on a synthetic carbonated nanocrystalline apatite preliminarily characterized (by XRD, FTIR, Raman, TG-DTA and SEM-EDX) was investigated in detail, pointing out a good agreement with Sips isothermal features. Adsorption characteristics were compared to those previously obtained on monophosphate nucleotides (AMP, CMP), unveiling some specificities. ATP was found to adsorb effectively onto biomimetic apatite: despite smaller values of the affinity constant KS and the exponential factor m, larger adsorbed amounts were reached for ATP as compared to AMP for any given concentration in solution. m guided by direct surface bonding rather than through stabilizing intermolecular interactions. Although standard οGads ° was estimated to only ⿿4 kJ/mol, the large value of Nmax led to significantly negative effective οGads values down to ⿿33 kJ/mol, reflecting the spontaneous character of adsorption process. Vibrational spectroscopy data (FTIR and Raman) pointed out spectral modifications upon adsorption, confirming chemical-like interactions where both the triphosphate group of ATP and its nucleic base were involved. The present study is intended to serve as a basis for future research works involving ATP and apatite nanocrystals/nanoparticles in view of biomedical applications (e.g. bone tissue engineering, intracellular drug delivery, ⿦).

  19. Optimization of adenosine 5'-triphosphate extraction for the measurement of acidogenic biomass utilizing whey wastewater. (United States)

    Lee, Changsoo; Kim, Jaai; Hwang, Seokhwan


    A set of experiments was carried out to maximize adenosine 5'-triphosphate (ATP) extraction efficiency from acidogenic culture using whey wastewater. ATP concentrations at different microbial concentrations increased linearly as microbial concentration decreased. More than 50% of ATP was extracted from the sample of 39 mg volatile suspended solids (VSS)/l compared to the sample of 2.8 g VSS/l. The ATP concentrations of the corresponding samples were 0.74+/-0.06 and 0.49+/-0.05 mg/l, respectively. For low VSS concentrations ranging from 39 to 92 mg/l, the extracted ATP concentration did not vary significantly at 0.73+/-0.01 mg ATP/l. Response surface methodology with a central composite in cube design for the experiments was used to locate the optimum for maximal ATP extraction with respect to boiling and bead beating treatments. The overall designed intervals were from 0 to 15 min and from 0 to 3 min for boiling and bead beating, respectively. The extracted ATP concentration ranged from 0.01 to 0.74 mg/l within the design boundary. The following is a partial cubic model where eta is the concentration of ATP and x ( k ) is the corresponding variable term (k=boiling time and bead beating time in order): eta=0.629+0.035x (1)-0.818x (2)-0.002x (1) x (2)-0.003x (1) (2) +0.254x (2) (2) +0.002x (1) (2) x (2). This model successfully approximates the response of ATP concentration with respect to the boiling- and bead beating-time. The condition for maximal ATP extraction was 5.6 min boiling without bead beating. The maximal ATP concentration using the model was 0.74 mg/l, which was identical to the experimental value at optimum condition for ATP extraction.

  20. Thiamine triphosphate: a ubiquitous molecule in search of a physiological role. (United States)

    Bettendorff, Lucien; Lakaye, Bernard; Kohn, Gregory; Wins, Pierre


    Thiamine triphosphate (ThTP) was discovered over 60 years ago and it was long thought to be a specifically neuroactive compound. Its presence in most cell types, from bacteria to mammals, would suggest a more general role but this remains undefined. In contrast to thiamine diphosphate (ThDP), ThTP is not a coenzyme. In E. coli cells, ThTP is transiently produced in response to amino acid starvation, while in mammalian cells, it is constitutively produced at a low rate. Though it was long thought that ThTP was synthesized by a ThDP:ATP phosphotransferase, more recent studies indicate that it can be synthesized by two different enzymes: (1) adenylate kinase 1 in the cytosol and (2) FoF1-ATP synthase in brain mitochondria. Both mechanisms are conserved from bacteria to mammals. Thus ThTP synthesis does not seem to require a specific enzyme. In contrast, its hydrolysis is catalyzed, at least in mammalian tissues, by a very specific cytosolic thiamine triphosphatase (ThTPase), controlling the steady-state cellular concentration of ThTP. In some tissues where adenylate kinase activity is high and ThTPase is absent, ThTP accumulates, reaching ≥ 70% of total thiamine, with no obvious physiological consequences. In some animal tissues, ThTP was able to phosphorylate proteins, and activate a high-conductance anion channel in vitro. These observations raise the possibility that ThTP is part of a still uncharacterized cellular signaling pathway. On the other hand, its synthesis by a chemiosmotic mechanism in mitochondria and respiring bacteria might suggest a role in cellular energetics.

  1. Uridine Triphosphate Thio Analogues Inhibit Platelet P2Y12 Receptor and Aggregation (United States)

    Gündüz, Dursun; Tanislav, Christian; Sedding, Daniel; Parahuleva, Mariana; Santoso, Sentot; Troidl, Christian; Hamm, Christian W.; Aslam, Muhammad


    Platelet P2Y12 is an important adenosine diphosphate (ADP) receptor that is involved in agonist-induced platelet aggregation and is a valuable target for the development of anti-platelet drugs. Here we characterise the effects of thio analogues of uridine triphosphate (UTP) on ADP-induced platelet aggregation. Using human platelet-rich plasma, we demonstrate that UTP inhibits P2Y12 but not P2Y1 receptors and antagonises 10 µM ADP-induced platelet aggregation in a concentration-dependent manner with an IC50 value of ~250 µM. An eight-fold higher platelet inhibitory activity was observed with a 2-thio analogue of UTP (2S-UTP), with an IC50 of 30 µM. The 4-thio analogue (4S-UTP) with an IC50 of 7.5 µM was 33-fold more effective. A three-fold decrease in inhibitory activity, however, was observed by introducing an isobutyl group at the 4S- position. A complete loss of inhibition was observed with thio-modification of the γ phosphate of the sugar moiety, which yields an enzymatically stable analogue. The interaction of UTP analogues with P2Y12 receptor was verified by P2Y12 receptor binding and cyclic AMP (cAMP) assays. These novel data demonstrate for the first time that 2- and 4-thio analogues of UTP are potent P2Y12 receptor antagonists that may be useful for therapeutic intervention. PMID:28146050

  2. Antihyperlipidemic activity of adenosine triphosphate in rabbits fed a high-fat diet and hyperlipidemic patients. (United States)

    Zhang, Lianshan; Liang, Libin; Tong, Tong; Qin, Yuguo; Xu, Yanping; Tong, Xinglong


    Context Recently, adenosine triphosphate (ATP) was occasionally found to decrease the triglyceride (TG) levels in several hyperlipidemic patients in our clinical practice. Objective The study investigates the anti-hyperlipidemic effects of ATP in a high-fat fed rabbit model and hyperlipidemic patients. Materials and methods Twenty-four rabbits were randomly divided into three groups of eight animals each as follows: normal diet, high-fat diet and high-fat diet + ATP group. ATP supplementation (40 mg/day) was started at the 20th day and lasted for 10 days. Serum concentrations of total cholesterol (TC), TG, LDL-C, HDL-C were measured on the 20th day and 30th day. Heart, liver and aorta were subjected histopathological examination. Twenty outpatients diagnosed primary hyperlipidemia took ATP at a dose of 60 mg twice a day for 1 week. Results Feeding rabbits with a high-fat diet resulted in a significant elevation of lipid parameters including TC, TG, LDL-C, VLDL-C compared to the normal diet group (p < 0.01). ATP treatment significantly decreased serum TG level (p < 0.01), whilst other parameters remained statistically unaltered. Meanwhile, ATP significantly reduced the thickness of fat layer in cardiac epicardium (p < 0.05) and pathological gradation of ballooning degeneration in hepatocytes (p < 0.05). After taking ATP for 1 week, hyperlipidemia patients exhibited a significant decrease of TG (p < 0.01), but other lipid parameters had no significant change. Discussion and conclusion The study indicates that ATP selectively decreases serum TG levels in high-fat diet rabbits and hyperlipidemic patients. Therefore, ATP supplementation may provide an effective approach to control TG level.

  3. SNC-80-induced preconditioning: selective activation of the mitochondrial adenosine triphosphate-gated potassium channel. (United States)

    Fischbach, Peter S; Barrett, Terrance D; Reed, Nathan J; Lucchesi, Benedict R


    Pharmacologic preconditioning by delta-opioid agonists occurs via activation of an adenosine triphosphate (ATP)-gated potassium channel (I(KATP)). Opening of mitochondrial I(KATP) confers pharmacologic preconditioning whereas opening the sarcolemmal I(KATP) shortens action potential duration and is proarrhythmic. This study investigated whether SNC-80, a selective delta-opioid agonist, is associated with development of ventricular arrhythmia due to activation of I(KATP). Rabbit isolated hearts were subjected to 12 min of hypoxia and 40 min of reoxygenation after pretreatment with SNC-80 (1 microM, n = 6), pinacidil (1.25 microM, n = 12), or BMS-191095 (6.0 microM, n = 4). Nine additional hearts served as controls. The cytoprotective effects of SNC-80 at a concentration of 1 microM were confirmed using 30 min of regional ischemia followed by 120 min of reperfusion. Ventricular fibrillation (VF) developed in 11 of 12 pinacidil-treated hearts whereas none of the SNC-80-treated (zero of six) hearts developed VF (P SNC-80 reduced infarct size expressed as a percentage of the area at risk from 33 +/- 4% to 14 +/- 3% (P = 0.004) compared with control. SNC-80, which selectively activates the delta-opioid receptor, provided cytoprotection but did not induce VF after hypoxia reoxygenation. The results indicate that pinacidil-induced nonselective activation of I(KATP) results in proarrhythmia that is dependent on activation of the sarcolemmal I(KATP). Selectivity for the mitochondrial I(KATP) is necessary to prevent induction of a proarrhythmic state.

  4. Extracellular adenosine 5'-triphosphate and lipopolysaccharide induce interleukin-1β release in canine blood. (United States)

    Spildrejorde, Mari; Curtis, Stephen J; Curtis, Belinda L; Sluyter, Ronald


    Binding of extracellular adenosine 5'-triphosphate (ATP) or lipopolysaccharide (LPS) to the damage-associated molecular pattern receptor P2X7 or the pathogen-associated molecular pattern receptor Toll-like receptor (TLR)4, respectively, can induce the release of the pleiotropic cytokine interleukin (IL)-1β in humans and mice. However, the release of IL-1β in dogs remains poorly defined. Using a canine IL-1β enzyme-linked immunosorbent assay, this study investigated whether ATP or LPS could induce IL-1β release in a canine blood-based assay. Short-term incubations (30 min) with ATP induced IL-1β release in LPS-primed canine blood, and this process could be near-completely impaired by the P2X7 antagonist, A438079. In contrast, ATP failed to induce IL-1β release from blood not primed with LPS. ATP-induced IL-1β release was observed with LPS-primed blood from eight different pedigrees or cross breeds. Long-term incubations (24h) with LPS induced IL-1β release in canine blood in a concentration-dependent manner. This process was not altered by co-incubation with A438079. LPS-induced IL-1β release was observed with blood from 10 different pedigrees or cross breeds. These results demonstrate that both extracellular ATP and LPS can induce IL-1β release in dogs, and that ATP- but not LPS-induced IL-1β release in blood is dependent on P2X7 activation. These findings support the role of both P2X7 and TLR4 in IL-1β release in dogs.

  5. Free Surface Water Tunnel (FSWT) (United States)

    Federal Laboratory Consortium — Description: The Free Surface Water Tunnel consists of the intake plenum, the test section and the exit plenum. The intake plenum starts with a perforated pipe that...

  6. The Statistics of Chaotic Tunnelling

    CERN Document Server

    Creagh, S C; Creagh, Stephen C.; Whelan, Niall D.


    We discuss the statistics of tunnelling rates in the presence of chaotic classical dynamics. This applies to resonance widths in chaotic metastable wells and to tunnelling splittings in chaotic symmetric double wells. The theory is based on using the properties of a semiclassical tunnelling operator together with random matrix theory arguments about wave function overlaps. The resulting distribution depends on the stability of a specific tunnelling orbit and is therefore not universal. However it does reduce to the universal Porter-Thomas form as the orbit becomes very unstable. For some choices of system parameters there are systematic deviations which we explain in terms of scarring of certain real periodic orbits. The theory is tested in a model symmetric double well problem and possible experimental realisations are discussed.

  7. Self-Organizing Tunnel Peers

    Institute of Scientific and Technical Information of China (English)


    Tunneling is an important approach in IPv6 transition techniques. The tunnel broker model provides a way to build virtual IPv6 networks without manual configuration.However, neither it adapts performance variation on the IPv4 infrastructure,nor it is a scalable solution for a wide-area IPv6 networking environment. In this paper, a self-organizing tunnel peer (SOTP)model is presented. Tunnel peers are clustered in the SOTP system so that optimization is scalable. Four primitive operations related to cluster construction - arrest,release,division and death - endow the system with the nature of self-organization.Occurrence and behavior of the operations are decided by criteria on the IPv4 end-to-end performance; hence measurement is an indispensable component of the system. The metabolism of cluster relaxes the requirement to accuracy of measurement and optimization.

  8. Flatback airfoil wind tunnel experiment.

    Energy Technology Data Exchange (ETDEWEB)

    Mayda, Edward A. (University of California, Davis, CA); van Dam, C.P. (University of California, Davis, CA); Chao, David D. (University of California, Davis, CA); Berg, Dale E.


    A computational fluid dynamics study of thick wind turbine section shapes in the test section of the UC Davis wind tunnel at a chord Reynolds number of one million is presented. The goals of this study are to validate standard wind tunnel wall corrections for high solid blockage conditions and to reaffirm the favorable effect of a blunt trailing edge or flatback on the performance characteristics of a representative thick airfoil shape prior to building the wind tunnel models and conducting the experiment. The numerical simulations prove the standard wind tunnel corrections to be largely valid for the proposed test of 40% maximum thickness to chord ratio airfoils at a solid blockage ratio of 10%. Comparison of the computed lift characteristics of a sharp trailing edge baseline airfoil and derived flatback airfoils reaffirms the earlier observed trend of reduced sensitivity to surface contamination with increasing trailing edge thickness.

  9. 5-foot Vertical Wind Tunnel (United States)


    The researcher is sitting above the exit cone of the 5-foot Vertical Wind Tunnel and is examining the new 6-component spinning balance. This balance was developed between 1930 and 1933. It was an important advance in the technology of rotating or rolling balances. As M.J. Bamber and C.H. Zimmerman wrote in NACA TR 456: 'Data upon the aerodynamic characteristics of a spinning airplane may be obtained in several ways; namely, flight tests with full-scale airplanes, flight tests with balanced models, strip-method analysis of wind-tunnel force and moment tests, and wind-tunnel tests of rotating models.' Further, they note: 'Rolling-balance data have been of limited value because it has not been possible to measure all six force and moment components or to reproduce a true spinning condition. The spinning balance used in this investigation is a 6-component rotating balance from which it is possible to obtain wind-tunnel data for any of a wide range of possible spinning conditions.' Bamber and Zimmerman described the balance as follows: 'The spinning balance consists of a balance head that supports the model and contains the force-measuring units, a horizontal turntable supported by streamline struts in the center of the jet and, outside the tunnel, a direct-current driving motor, a liquid tachometer, an air compressor, a mercury manometer, a pair of indicating lamps, and the necessary controls. The balance head is mounted on the turntable and it may be set to give any radius of spin between 0 and 8 inches.' In an earlier report, NACA TR 387, Carl Wenzinger and Thomas Harris supply this description of the tunnel: 'The vertical open-throat wind tunnel of the National Advisory Committee for Aeronautics ... was built mainly for studying the spinning characteristics of airplane models, but may be used as well for the usual types of wind-tunnel tests. A special spinning balance is being developed to measure the desired forces and moments with the model simulating the actual

  10. Sensitive, Rapid Detection of Bacterial Spores (United States)

    Kern, Roger G.; Venkateswaran, Kasthuri; Chen, Fei; Pickett, Molly; Matsuyama, Asahi


    A method of sensitive detection of bacterial spores within delays of no more than a few hours has been developed to provide an alternative to a prior three-day NASA standard culture-based assay. A capability for relatively rapid detection of bacterial spores would be beneficial for many endeavors, a few examples being agriculture, medicine, public health, defense against biowarfare, water supply, sanitation, hygiene, and the food-packaging and medical-equipment industries. The method involves the use of a commercial rapid microbial detection system (RMDS) that utilizes a combination of membrane filtration, adenosine triphosphate (ATP) bioluminescence chemistry, and analysis of luminescence images detected by a charge-coupled-device camera. This RMDS has been demonstrated to be highly sensitive in enumerating microbes (it can detect as little as one colony-forming unit per sample) and has been found to yield data in excellent correlation with those of culture-based methods. What makes the present method necessary is that the specific RMDS and the original protocols for its use are not designed for discriminating between bacterial spores and other microbes. In this method, a heat-shock procedure is added prior to an incubation procedure that is specified in the original RMDS protocols. In this heat-shock procedure (which was also described in a prior NASA Tech Briefs article on enumerating sporeforming bacteria), a sample is exposed to a temperature of 80 C for 15 minutes. Spores can survive the heat shock, but nonspore- forming bacteria and spore-forming bacteria that are not in spore form cannot survive. Therefore, any colonies that grow during incubation after the heat shock are deemed to have originated as spores.

  11. Submucosal tunneling techniques: current perspectives

    Directory of Open Access Journals (Sweden)

    Kobara H


    Full Text Available Hideki Kobara,1 Hirohito Mori,1 Kazi Rafiq,2 Shintaro Fujihara,1 Noriko Nishiyama,1 Maki Ayaki,1 Tatsuo Yachida,1 Tae Matsunaga,1 Johji Tani,1 Hisaaki Miyoshi,1 Hirohito Yoneyama,1 Asahiro Morishita,1 Makoto Oryu,1 Hisakazu Iwama,3 Tsutomu Masaki1 1Department of Gastroenterology and Neurology, 2Department of Pharmacology, 3Life Science Research Center, Faculty of Medicine, Kagawa University, Miki-cho, Kita-Gun, Kagawa, Japan Abstract: Advances in endoscopic submucosal dissection include a submucosal tunneling technique, involving the introduction of tunnels into the submucosa. These tunnels permit safer offset entry into the peritoneal cavity for natural orifice transluminal endoscopic surgery. Technical advantages include the visual identification of the layers of the gut, blood vessels, and subepithelial tumors. The creation of a mucosal flap that minimizes air and fluid leakage into the extraluminal cavity can enhance the safety and efficacy of surgery. This submucosal tunneling technique was adapted for esophageal myotomy, culminating in its application to patients with achalasia. This method, known as per oral endoscopic myotomy, has opened up the new discipline of submucosal endoscopic surgery. Other clinical applications of the submucosal tunneling technique include its use in the removal of gastrointestinal subepithelial tumors and endomicroscopy for the diagnosis of functional and motility disorders. This review suggests that the submucosal tunneling technique, involving a mucosal safety flap, can have potential values for future endoscopic developments. Keywords: submucosal endoscopy, submucosal tunneling method, natural orifice transluminal endoscopic surgery, peroral endoscopic myotomy, gastrointestinal subepithelial tumor, functional and motility disorders

  12. Molecular structure of tetraaqua adenosine 5'-triphosphate aluminium(III) complex: a study involving Raman spectroscopy, theoretical DFT and potentiometry. (United States)

    Tenório, Thaís; Silva, Andréa M; Ramos, Joanna Maria; Buarque, Camilla D; Felcman, Judith


    The Alzheimer's disease is one of the most common neurodegenerative diseases that affect elderly population, due to the formation of β-amyloid protein aggregate and several symptoms, especially progressive cognitive decline. The result is a decrease in capture of glucose by cells leading to obliteration, meddling in the Krebs cycle, the principal biochemical route to the energy production leading to a decline in the levels of adenosine 5'-triphosphate. Aluminium(III) is connected to Alzheimer's and its ion provides raise fluidity of the plasma membrane, decrease cell viability and aggregation of amyloid plaques. Studies reveal that AlATP complex promotes the formation of reactive fibrils of β-amyloid protein and independent amyloidogenic peptides, suggesting the action of the complex as a chaperone in the role pathogenic process. In this research, one of complexes formed by Al(III) and adenosine 5'-triphosphate in aqueous solution is analyzed by potentiometry, Raman spectroscopy and ab initio calculations. The value of the logK(AlATP) found was 9.21±0.01 and adenosine 5'-triphosphate should act as a bidentate ligand in the complex. Raman spectroscopy and potentiometry indicate that donor atoms are the oxygen of the phosphate β and the oxygen of the phosphate γ, the terminal phosphates. Computational calculations using Density Functional Theory, with hybrid functions B3LYP and 6-311++G(d,p) basis set regarding water solvent effects, have confirmed the results. Frontier molecular orbitals, electrostatic potential contour surface, electrostatic potential mapped and Mulliken charges of the title molecule were also investigated.

  13. Cytosolic 5'-triphosphate ended viral leader transcript of measles virus as activator of the RIG I-mediated interferon response.

    Directory of Open Access Journals (Sweden)

    Sébastien Plumet

    Full Text Available BACKGROUND: Double stranded RNA (dsRNA is widely accepted as an RNA motif recognized as a danger signal by the cellular sentries. However, the biology of non-segmented negative strand RNA viruses, or Mononegavirales, is hardly compatible with the production of such dsRNA. METHODOLOGY AND PRINCIPAL FINDINGS: During measles virus infection, the IFN-beta gene transcription was found to be paralleled by the virus transcription, but not by the virus replication. Since the expression of every individual viral mRNA failed to activate the IFN-beta gene, we postulated the involvement of the leader RNA, which is a small not capped and not polyadenylated RNA firstly transcribed by Mononegavirales. The measles virus leader RNA, synthesized both in vitro and in vivo, was efficient in inducing the IFN-beta expression, provided that it was delivered into the cytosol as a 5'-trisphosphate ended RNA. The use of a human cell line expressing a debilitated RIG-I molecule, together with overexpression studies of wild type RIG-I, showed that the IFN-beta induction by virus infection or by leader RNA required RIG-I to be functional. RIG-I binds to leader RNA independently from being 5-trisphosphate ended; while a point mutant, Q299A, predicted to establish contacts with the RNA, fails to bind to leader RNA. Since the 5'-triphosphate is required for optimal RIG-I activation but not for leader RNA binding, our data support that RIG-I is activated upon recognition of the 5'-triphosphate RNA end. CONCLUSIONS/SIGNIFICANCE: RIG-I is proposed to recognize Mononegavirales transcription, which occurs in the cytosol, while scanning cytosolic RNAs, and to trigger an IFN response when encountering a free 5'-triphosphate RNA resulting from a mislocated transcription activity, which is therefore considered as the hallmark of a foreign invader.

  14. Molecular structure of tetraaqua adenosine 5'-triphosphate aluminium(III) complex: A study involving Raman spectroscopy, theoretical DFT and potentiometry (United States)

    Tenório, Thaís; Silva, Andréa M.; Ramos, Joanna Maria; Buarque, Camilla D.; Felcman, Judith


    The Alzheimer's disease is one of the most common neurodegenerative diseases that affect elderly population, due to the formation of β-amyloid protein aggregate and several symptoms, especially progressive cognitive decline. The result is a decrease in capture of glucose by cells leading to obliteration, meddling in the Krebs cycle, the principal biochemical route to the energy production leading to a decline in the levels of adenosine 5'-triphosphate. Aluminium(III) is connected to Alzheimer's and its ion provides raise fluidity of the plasma membrane, decrease cell viability and aggregation of amyloid plaques. Studies reveal that AlATP complex promotes the formation of reactive fibrils of β-amyloid protein and independent amyloidogenic peptides, suggesting the action of the complex as a chaperone in the role pathogenic process. In this research, one of complexes formed by Al(III) and adenosine 5'-triphosphate in aqueous solution is analyzed by potentiometry, Raman spectroscopy and ab initio calculations. The value of the log KAlATP found was 9.21 ± 0.01 and adenosine 5'-triphosphate should act as a bidentate ligand in the complex. Raman spectroscopy and potentiometry indicate that donor atoms are the oxygen of the phosphate β and the oxygen of the phosphate γ, the terminal phosphates. Computational calculations using Density Functional Theory, with hybrid functions B3LYP and 6-311++G(d,p) basis set regarding water solvent effects, have confirmed the results. Frontier molecular orbitals, electrostatic potential contour surface, electrostatic potential mapped and Mulliken charges of the title molecule were also investigated.

  15. Caenorhabditis elegans inositol 5-phosphatase homolog negatively regulates inositol 1,4,5-triphosphate signaling in ovulation. (United States)

    Bui, Yen Kim; Sternberg, Paul W


    Ovulation in Caenorhabditis elegans requires inositol 1,4,5-triphosphate (IP(3)) signaling activated by the epidermal growth factor (EGF)-receptor homolog LET-23. We generated a deletion mutant of a type I 5-phosphatase, ipp-5, and found a novel ovulation phenotype whereby the spermatheca hyperextends to engulf two oocytes per ovulation cycle. The temporal and spatial expression of IPP-5 is consistent with its proposed inhibition of IP(3) signaling in the adult spermatheca. ipp-5 acts downstream of let-23, and interacts with let-23-mediated IP(3) signaling pathway genes. We infer that IPP-5 negatively regulates IP(3) signaling to ensure proper spermathecal contraction.

  16. TunnelVision: LHC Tunnel Photogrammetry System for Structural Monitoring

    CERN Document Server

    Fallas, William


    In this document an algorithm to detect deformations in the LHC Tunnel of CERN is presented. It is based on two images, one represents the ideal state of the tunnel and the other one the actual state. To find the differences between both, the algorithm is divided in three steps. First, an image enhancement is applied to make easier the detection. Second, two different approaches to reduce noise are applied to one or both images. And third, it is defined a group of characteristics about the type of deformation desired to detect. Finally, the conclusions show the effectiveness of the algorithm in the experimental results.

  17. Use of a Novel 5′-Regioselective Phosphitylating Reagent for One-Pot Synthesis of Nucleoside 5′-Triphosphates from Unprotected Nucleosides (United States)

    Caton-Williams, Julianne; Hoxhaj, Rudiona; Fiaz, Bilal


    The 5′-triphosphates are the building blocks for the enzymatic synthesis of DNAs and RNAs. This unit presents a protocol for the convenient synthesis of 2′-deoxyribo- and ribo-nucleoside 5′-triphosphates (dNTPs and NTPs) containing all the natural bases and the modified bases. This one-pot synthesis can also be applied to prepare the triphosphate analogs that contain sulfur or selenium atoms replacing the non-bridging oxygen atoms of the alpha phosphates of the triphosphates. These S- or Se-modified dNTPs and NTPs can be used to prepare diastereomerically-pure phosphorothioate nucleic acids (PS-NAs) or phosphoroselenoate nucleic acids (PSe-NAs, i.e., one type of selenium-derivatized nucleic acids: SeNA). Even without extensive purification, the synthesized dNTPs or NTPs are of high quality and can be directly used in DNA polymerization or RNA transcription. Synthesis and purification of the 5′-triphosphates, analysis and confirmation of natural and sulfur-or selenium-modified nucleic acids are described in this protocol unit. PMID:23512692

  18. Design of tunneling injection quantum dot lasers

    Institute of Scientific and Technical Information of China (English)

    JIA Guo-zhi; YAO Jiang-hong; SHU Yong-chun; WANG Zhan-guo


    To implement high quality tunneling injection quantum dot lasers,effects of primary factors on performance of the tunneling injection quantum dot lasers were investigated. The considered factors were tunneling probability,tunneling time and carriers thermal escape time from the quantum well. The calculation results show that with increasing of the ground-state energy level in quantum well,the tunneling probability increases and the tunneling time decreases,while the thermal escape time decreases because the ground-state energy levelis shallower. Longitudinal optical phonon-assisted tunneling can be an effective method to solve the problem that both the tunneling time and the thermal escape time decrease simultaneously with the ground-state energy level increasing in quantum well.

  19. Ecto-nucleoside triphosphate diphosphohydrolase 2 modulates local ATP-induced calcium signaling in human HaCaT keratinocytes.

    Directory of Open Access Journals (Sweden)

    Chia-Lin Ho

    Full Text Available Keratinocytes are the major building blocks of the human epidermis. In many physiological and pathophysiological conditions, keratinocytes release adenosine triphosphate (ATP as an autocrine/paracrine mediator that regulates cell proliferation, differentiation, and migration. ATP receptors have been identified in various epidermal cell types; therefore, extracellular ATP homeostasis likely determines its long-term, trophic effects on skin health. We investigated the possibility that human keratinocytes express surface-located enzymes that modulate ATP concentration, as well as the corresponding receptor activation, in the pericellular microenvironment. We observed that the human keratinocyte cell line HaCaT released ATP and hydrolyzed extracellular ATP. Interestingly, ATP hydrolysis resulted in adenosine diphosphate (ADP accumulation in the extracellular space. Pharmacological inhibition by ARL 67156 or gene silencing of the endogenous ecto-nucleoside triphosphate diphosphohydrolase (NTPDase isoform 2 resulted in a 25% reduction in both ATP hydrolysis and ADP formation. Using intracellular calcium as a reporter, we found that although NTPDase2 hydrolyzed ATP and generated sustainable ADP levels, only ATP contributed to increased intracellular calcium via P2Y2 receptor activation. Furthermore, knocking down NTPDase2 potentiated the nanomolar ATP-induced intracellular calcium increase, suggesting that NTPDase2 globally attenuates nucleotide concentration in the pericellular microenvironment as well as locally shields receptors in the vicinity from being activated by extracellular ATP. Our findings reveal an important role of human keratinocyte NTPDase2 in modulating nucleotide signaling in the extracellular milieu of human epidermis.

  20. Tunneling field effect transistor technology

    CERN Document Server

    Chan, Mansun


    This book provides a single-source reference to the state-of-the art in tunneling field effect transistors (TFETs). Readers will learn the TFETs physics from advanced atomistic simulations, the TFETs fabrication process and the important roles that TFETs will play in enabling integrated circuit designs for power efficiency. · Provides comprehensive reference to tunneling field effect transistors (TFETs); · Covers all aspects of TFETs, from device process to modeling and applications; · Enables design of power-efficient integrated circuits, with low power consumption TFETs.

  1. Spin tunnelling in mesoscopic systems

    Indian Academy of Sciences (India)

    Anupam Garg


    We study spin tunnelling in molecular magnets as an instance of a mesoscopic phenomenon, with special emphasis on the molecule Fe8. We show that the tunnel splitting between various pairs of Zeeman levels in this molecule oscillates as a function of applied magnetic field, vanishing completely at special points in the space of magnetic fields, known as diabolical points. This phenomena is explained in terms of two approaches, one based on spin-coherent-state path integrals, and the other on a generalization of the phase integral (or WKB) method to difference equations. Explicit formulas for the diabolical points are obtained for a model Hamiltonian.

  2. Quantum Cosmology for Tunneling Universes

    CERN Document Server

    Kim, Sang Pyo


    In a quantum cosmological model consisting of a Euclidean region and a Lorentzian region, Hartle-Hawking's no-bounary wave function, and Linde's wave function and Vilenkin's tunneling wave function are briefly described and compared with each other. We put a particular emphasis on semiclassical gravity from quantum cosmology and compare it with the conventional quantum field theory in curved spacetimes. Finally, we discuss the recent debate on catastrophic particle production in the tunneling universe between Rubakov and Vilenkin within the semiclassical gravity.

  3. Drill and blast tunnelling; Konvensjonell drift av tunneler

    Energy Technology Data Exchange (ETDEWEB)

    Roenn, Paal-Egil


    This thesis treats drill and blast tunnelling. The rapid technological advance necessitates revised and updated design criteria, quality requirements and quality control. In situ blast experiments were carried out in order to test new methods and improve the basis for calculation and design. The main topics of the experiments were (1) longer rounds and increased drillhole diameter, (2) emulsion slurry as explosives in tunnelling, and (3) electronic detonators in contour blasting. The experiments show that it is technically feasible to blast rounds of up to 8.6 m length. Using current technology, the economical optimum round length is substantially shorter. Dust, low visibility, noise and toxic fumes are occupational environmental strains for the tunnel workers. Several of the environmental factors are strongly influenced by the type of explosives used. For example, emulsion slurry resulted in 4 to 5 times better visibility than Anolit and the concentration of respirable dust and total dust was reduced by 30-50 %. Electronic detonators were tested and found to give a higher percentage of remaining drillholes in the contour than Nonel detonators. The thesis includes a chapter on economic design of hydropower tunnels. 42 refs., 83 figs., 45 tabs.

  4. Fiber coupled ultrafast scanning tunneling microscope

    DEFF Research Database (Denmark)

    Keil, Ulrich Dieter Felix; Jensen, Jacob Riis; Hvam, Jørn Märcher


    We report on a scanning tunneling microscope with a photoconductive gate in the tunneling current circuit. The tunneling tip is attached to a coplanar transmission line with an integrated photoconductive switch. The switch is illuminated through a fiber which is rigidly attached to the switch...

  5. A Wind Tunnel Captive Aircraft Testing Technique (United States)


    Flight/Wind Tunnel Correlation of Aircraft Longitudinal Motion ....................................... 14 10. Fright/Wind Tunnel Correlation of...I 2 3 4 5 6 T IME, s e c Figure 9. Flight/wind tunnel correla- tion of aircraft longitudinal motion. ’ D A n ~ v i i i | ~ 0 0 - 4 0

  6. 78 FR 46117 - National Tunnel Inspection Standards (United States)


    ... demonstrate long-term accountability and accomplishment. To meet the needs of this management approach, the... ceilings in the D Street portal tunnels, the anchor creep that led to this accident would likely have been... border. Tests of 26 tunnels in 13 European countries in 2010 by the European Tunnel Assessment...

  7. Tunnel Face Stability & New CPT Applications

    NARCIS (Netherlands)

    Broere, W.


    Nearly all tunnels bored in soft soils have encountered problems with the stability of the tunnel face. In several cases these problems led to an extended stand-still of the boring process. A better understanding of the face stability, and of the soil conditions around the tunnel boring machine, can

  8. Association study on adenosine triphosphate biofluorescence detection technology%ATP生物荧光检测技术相关性基础研究

    Institute of Scientific and Technical Information of China (English)

    易滨; 刘军; 王芳; 涂显春; 赵晓晓; 赵江丽


    Objective To test the relation between adenosine triphosphate (ATP) biofluorescence detection technology and bacterial colony forming unit (CFU) and blood content Methods Escherichia coli (E. coli) ATCC 8099 and heathy controls' blood were diluted to the concentration of 10"' ,10-2,10-3,10-4,10-5,10-6 and 10-7', respectively, then lysate, luciferase and ATP standard substance were added, relative light unit (RLU) values were determined twice by fluorimeter, ATP contents(amol) were converted according to formulaCATP content = A1/A2-A1 × 106); hemoglobin values were measured by hematology analyzer to reflect protein residue leveL Curve demarcate standardization was made to show the relation between diluted bacteria, hemoglobin content and ATP content. Results Bacterial CFU and ATP content logarithm values (y = 1. 07x - 0. 55, R2 = 0. 99), bacterial CFU and ATP RLU logarithm values (y=1.14x + 0. 33,R2 =0. 99) showed linear relationship respectively. There was no significant difference between the logical values figured out by different RLU values and the actual values. After hemodilution, hemoglobin and ATP RLU logarithm values also showed linear relationship (y= 1. 03x-8. 42, R2 =0. 99). Conclusion ATP biofluorescence detection technology can detect the content of bacteria and protein through ATP value and RLU, it can determine contamination degree and clean effect of medical equipments and object surface, it's a new, sensitive and rapid detection method.%目的 测试三磷酸腺苷(ATP)生物荧光检测技术与菌落数和血液含量的关系.方法 将大肠埃希菌ATCC 8099菌液和健康人的血液分别稀释为10-1、10-2、10-3、10-4、10-5、10-6、10-7,加入裂解液、酶、ATP标准品,用荧光光度计测定2次相对发光值(RLU),按照公式(ATP含量=A1/A2-A1×106)换算成ATP含量(amol);用血细胞分析仪测定血红蛋白值,以反映蛋白残留量.将各稀释度菌液、血红蛋白含量与ATP含量之间的关系进行曲线

  9. Time tunnels meet warped passages

    CERN Multimedia

    Kushner, David


    "Just in time for its 40th anniversary, the classic sci-fi television show "The time tunnel" is out on DVD. The conceit is something every engineer can relate to: a pulled plug. Scientists in an underground lab are working on a secret government experiment in time travel. (1 page)

  10. Installation in the SPS tunnel

    CERN Multimedia


    The SPS tunnel is 6910 m in circumference and has a cross section of 4 m inner diameter. It is situated at an elevation of 400 m above sea level at a depth below the surface varying between 23 and 65 m. Its walls are lined with a concrete shell of about 30 cm thickness. See also 7410043X

  11. Tunneling in the SIS Structure (United States)

    Shukrinov, Yu.; Kohandel, M.


    We discuss the effects caused by the layered structure of high temperature superconductors (HTS). We use the layered S-N model to obtain the tunneling current of the SIS structure. The current-voltage characteristic is calculated in the limit cases when dI/dV is proportional to the state density of HTS.

  12. Apparent tunneling in chemical reactions

    DEFF Research Database (Denmark)

    Henriksen, Niels Engholm; Hansen, Flemming Yssing; Billing, G. D.


    A necessary condition for tunneling in a chemical reaction is that the probability of crossing a barrier is non-zero, when the energy of the reactants is below the potential energy of the barrier. Due to the non-classical nature (i.e, momentum uncertainty) of vibrational states this is, however...

  13. Josephson tunnel junction microwave attenuator

    DEFF Research Database (Denmark)

    Koshelets, V. P.; Shitov, S. V.; Shchukin, A. V.


    A new element for superconducting electronic circuitry-a variable attenuator-has been proposed, designed, and successfully tested. The principle of operation is based on the change in the microwave impedance of a superconductor-insulator-superconductor (SIS) Josephson tunnel junction when dc bias...

  14. Earth Pressure on Tunnel Crown

    DEFF Research Database (Denmark)

    Andersen, Lars

    Two different analyses have been carried out in order to find the vertical earth pressure, or overburden pressure, at the crown of a tunnel going through a dike. Firstly, a hand calculation is performed using a simple dispersion of the stresses over depth. Secondly, the finite‐element program...

  15. The recurrent carpal tunnel syndrome. (United States)

    Kern, B C; Brock, M; Rudolph, K H; Logemann, H


    Sixteen out of 720 patients with carpal tunnel syndrome who had undergone surgery since 1979 were reoperated for a "recurrence" (2.2%). Twelve of these patients had been originally operated on in our department. Thus, our own recurrence rate is 1.7%. Three patients deteriorated following surgery, 6 had an unsatisfactory improvement, and in 7 the symptoms recurred after initial improvement. Eight of the reoperated patients had a predisposing disease (terminal renal insufficiency, insulin-dependent diabetes mellitus, acromegaly). In 10 of the 16 cases the initial operation had been carried out by surgeons in the first three years of training. Reoperation revealed incomplete splitting of the transverse carpal ligament in 10 cases, compression of the median nerve by the scar in 4, injury of the muscular branch in 1, and an anatomical variant as cause of incomplete decompression in 1 patient. "Recurrences" after carpal tunnel surgery are predominantly due to inadequacies of the first procedure. A remarkable number of patients (50%) has predisposing diseases. Interfascicular or epineural neurolysis and complete exposure and neurolysis of the median nerve and its branches is necessary only in cases of recurrence. Their omission at the first surgery does not result in an increased recurrence rate. Our observations indicate that the number of operations for recurrent carpal tunnel syndrome can probably be reduced when the first operation is performed with care and experience. Patients with carpal tunnel syndrome secondary to a systemic disease are particularly at risk.

  16. Introduction to scanning tunneling microscopy

    CERN Document Server

    Chen, C Julian


    The scanning tunneling and the atomic force microscope, both capable of imaging individual atoms, were crowned with the Physics Nobel Prize in 1986, and are the cornerstones of nanotechnology today. This is a thoroughly updated version of this 'bible' in the field.

  17. Tunnel lighting in Europe (abridged).

    NARCIS (Netherlands)

    Schreuder, D.A.


    A set of principles embodied in 1971 recommendations of the cie (commission internationale de l'eclairage - international commission on lighting) is beginning to be generally accepted throughout europe, so that it is now possible to speak of a european system of tunnel lighting." the recommendations

  18. A Seamless Ubiquitous Telehealthcare Tunnel

    Directory of Open Access Journals (Sweden)

    Sao-Jie Chen


    Full Text Available Mobile handheld devices are rapidly using to implement healthcare services around the World. Fundamentally, these services utilize telemedicine technologies. A disconnection of a mobile telemedicine system usually results in an interruption, which is embarrassing, and reconnection is necessary during the communication session. In this study, the Stream Control Transmission Protocol (SCTP is adopted to build a stable session tunnel to guarantee seamless switching among heterogeneous wireless communication standards, such as Wi-Fi and 3G. This arrangement means that the telemedicine devices will not be limited by a fixed wireless connection and can switch to a better wireless channel if necessary. The tunnel can transmit plain text, binary data, and video streams. According to the evaluation of the proposed software-based SCTP-Tunnel middleware shown, the performance is lower than anticipated and is slightly slower than a fixed connection. However, the transmission throughput is still acceptable for healthcare professionals in a healthcare enterprise or home care site. It is necessary to build more heterogeneous wireless protocols into the proposed tunnel-switching scheme to support all possible communication protocols. In addition, SCTP is another good choice for promoting communication in telemedicine and healthcare fields.

  19. Inelastic scattering in resonant tunneling

    DEFF Research Database (Denmark)

    Wingreen, Ned S.; Jacobsen, Karsten Wedel; Wilkins, John W.


    The exact resonant-tunneling transmission probability for an electron interacting with phonons is presented in the limit that the elastic coupling to the leads is independent of energy. The phonons produce transmission sidebands but do not affect the integrated transmission probability or the esc...

  20. Tunnel widening in anterior cruciate ligament reconstruction

    DEFF Research Database (Denmark)

    Clatworthy, M G; Annear, P; Bulow, J U


    .1% in the patella tendon group (P = film measurements. Tunnel widening did not correlate with the clinical findings, knee scores, KT-1000 or isokinetic muscle strength. Tunnel widening is marked in the hamstring group. Tunnel widening does not correlate with instability......We report a prospective series evaluating the incidence and degree of tunnel widening in a well-matched series of patients receiving a hamstring or patella tendon graft for anterior cruciate ligament (ACL) deficiency. We correlated tunnel widening with clinical factors, knee scores, KT-1000...

  1. An Entropic Formulation of Tunneling Time

    CERN Document Server

    Demir, Durmus A


    Quantum tunneling governs numerous phenomena in biology, chemistry, physics and technology. Tunneling time, formulated in various different forms due to the absence of a time operator in quantum theory, has been measured recently in experiments based on the attoclock in ultrafast laser ionization of Helium atoms [A.~Landsman {\\it et al.}, Optica {\\bf 1}, 343 (2014)]. The experiment performs a refined measurement with which no tunneling time formula in the literature exhibits adequate congruence. Here we show that, entropic considerations lead to a real tunneling time which shows remarkable agreement with the experimental data and stays always subluminal. Indeed, with phase space volume setting the number of microstates for a single evanescing particle in a state of definite momentum consistent with energy conservation, one is naturally led to a statistical description for quantum tunneling in which thermal energy sets the tunneling time. This entropic tunneling time is rather general and might also be extende...

  2. Resonant tunnel magnetoresistance in a double magnetic tunnel junction

    KAUST Repository

    Useinov, Arthur


    We present quasi-classical approach to calculate a spin-dependent current and tunnel magnetoresistance (TMR) in double magnetic tunnel junctions (DMTJ) FML/I/FMW/I/FMR, where the magnetization of the middle ferromagnetic metal layer FMW can be aligned parallel or antiparallel with respect to the fixed magnetizations of the left FML and right FMR ferromagnetic electrodes. The transmission coefficients for components of the spin-dependent current, and TMR are calculated as a function of the applied voltage. As a result, we found a high resonant TMR. Thus, DMTJ can serve as highly effective magnetic nanosensor for biological applications, or as magnetic memory cells by switching the magnetization of the inner ferromagnetic layer FMW.© Springer Science+Business Media, LLC 2011.


    Directory of Open Access Journals (Sweden)

    Zvonimir Deković


    Full Text Available he paper focuses on the features of management of tunnels on the Zagreb-Macelj motorway. Management of tunnels is represented trough public-private partnership model that is applied on the Zagreb-Macelj motorway. In the course of tunnel operation and maintenance in the first five years of tunnels exploitation, the emphasis is put on the maintenance of the tunnel equipment systems. Tunnels as part of the motorway alignment are the most demanding facilities for maintaining appropriate safety and operational level in order to rich continuous availability providing safe and quality of service to the motorway users. The goal of the tunnel operation and maintenance is to ensure undisturbed and safe traffic flow through the tunnels by keeping the tunnel at the normal functional conditions.

  4. In-Tunnel Blast Pressure Empirical Formulas for Detonations External, Internal and at the Tunnel Entrance

    Institute of Scientific and Technical Information of China (English)

    LI Xiudi; ZHENG Yingren


    In order to define the loading on protective doors of an underground tunnel,the exact knowledge of the blast propagation through tunnels is needed.Thirty-three scale high-explosive tests are conducted to obtain in-tunnel blast pressure for detonations external,internal and at the tunnel entrance.The cross section of the concrete model tunnel is 0.67 m2.Explosive charges of TNT,ranging in mass from 400 g to 4 600 g,are detonated at various positions along the central axis of the model tunnel.Blast gages are flush-installed in the interior surface of the tunnel to record side-on blast pressure as it propagates down the tunnel.The engineering empirical formulas for predicting blast peak pressure are evaluated,and are found to be reasonably accurate for in-tunnel pressure prediction.

  5. Utilization of adenosine triphosphate in rat mast cells during histamine release induced by the ionophore A23187

    DEFF Research Database (Denmark)

    Johansen, Torben


    The role of endogenous adenosine triphosphate (ATP) in histamine release from rat mast cells induced by the ionophore A23187 in vitro has been studied. 2 The amount of histamine released by calcium from rat mast cells primed with the ionophore A23187 was dependent on the ATP content of the mast...... cells. 3 In aerobic experiments a drastic reduction in mast cell ATP content was found during the time when histamine release induced by A23187 takes place. 4 Anaerobic experiments were performed with metabolic inhibitors (antimycin A, oligomycin, and carbonyl cyanide p......-trifluorometroxyphenylnydrazone), which are known to block the energy-dependent calcium uptake by isolated mitochondria. The mast cell ATP content was reduced during A23187-induced histamine release under anaerobic conditions in the presence of glucose. This indicates an increased utilization of ATP during the release process. 5...

  6. Adenosine triphosphate-binding cassette member A3 gene mutation in children from one family from Saudi Arabia

    Directory of Open Access Journals (Sweden)

    Gawahir Mohamed Ahmed Mukhtar


    Full Text Available Mutation in ABCA3, which is adenosine triphosphate-binding cassette member A3, a member of protein transporter family for phospholipids into the lamellar bodies during synthesis of surfactant, can cause lung disease related to surfactant dysfunction with autosomal recessive pattern. We reported three cases from same family with ABCA3 mutation, their gene, clinical course, and outcomes mentioning that one patient had successful lung transplantation, one started the process of the lung transplantation while the third one died during infancy. We concluded that the patients with ABCA3 gene mutations are increasing in numbers may be due to the availability of the genetic testing and high index of suspicion among physicians. Lung transplantation is the definitive treatment, but availability is limited in our region.

  7. Caenorhabditis elegans Inositol 5-Phosphatase Homolog Negatively Regulates Inositol 1,4,5-Triphosphate Signaling in Ovulation V⃞ (United States)

    Bui, Yen Kim; Sternberg, Paul W.


    Ovulation in Caenorhabditis elegans requires inositol 1,4,5-triphosphate (IP3) signaling activated by the epidermal growth factor (EGF)-receptor homolog LET-23. We generated a deletion mutant of a type I 5-phosphatase, ipp-5, and found a novel ovulation phenotype whereby the spermatheca hyperextends to engulf two oocytes per ovulation cycle. The temporal and spatial expression of IPP-5 is consistent with its proposed inhibition of IP3 signaling in the adult spermatheca. ipp-5 acts downstream of let-23, and interacts with let-23–mediated IP3 signaling pathway genes. We infer that IPP-5 negatively regulates IP3 signaling to ensure proper spermathecal contraction. PMID:12006659

  8. Prevention of bacterial adhesion

    DEFF Research Database (Denmark)

    Klemm, Per; Vejborg, Rebecca Munk; Hancock, Viktoria


    Management of bacterial infections is becoming increasingly difficult due to the emergence and increasing prevalence of bacterial pathogens that are resistant to available antibiotics. Conventional antibiotics generally kill bacteria by interfering with vital cellular functions, an approach...... that imposes selection pressure for resistant bacteria. New approaches are urgently needed. Targeting bacterial virulence functions directly is an attractive alternative. An obvious target is bacterial adhesion. Bacterial adhesion to surfaces is the first step in colonization, invasion, and biofilm formation....... As such, adhesion represents the Achilles heel of crucial pathogenic functions. It follows that interference with adhesion can reduce bacterial virulence. Here, we illustrate this important topic with examples of techniques being developed that can inhibit bacterial adhesion. Some of these will become...

  9. Simple detection of hepatitis C virus using {sup 125}I-2'-deoxyuridine triphosphate and gamma counter

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Soo Jin; Ahn, S. H.; Chung, W. S.; Woo, K. S.; Lim, S. J.; Choi, C. W.; Lim, S. M. [Korea Cancer Center Hospital, Seoul (Korea, Republic of)


    Hepatitis C Virus (HCV) is the major cause of post transfusion and sporadic non A, non B hepatitis. Current infection of HCV can be detected by PCR method. Using PCR, it has been possible to detect HCV viremia prior to immunological sero-conversion and to detect fluctuation of viremia in antibody-positive chronic HCV patients undergoing therapy with interferon. In this study, we established the simple method to detect HCV DNA by incorporation of {sup 125}I-deoxyuridine triphosphate(dUTP) into DNA during the PCR, and counted the radioactivity of PCR product by gamma counter. {sup 125}I-2'-deoxyuridine 5'-triphosphate was prepared, and incorporated into DNA during PCR. dUTP was radiolabeled by the iododemercuration of 5-mercuri intermediate. Iododemercuration labeling was completed with 98% yield and the obtained product was incorporated into DNA without further purification. After incorporation, covalently bonded radioiodine substituent was remained stable during PCR procedure HCV positive standard and positive patient sera in immunological assay were centrifuged. HCV RNA is isolated from by GTC(Guanidine Thiocyanate) and phenol/chloroform extraction method and synthesized complementary DNA by using reverse transcriptase. The '1{sup 25}I-dUTP was incorporated into HCV C DNA during PCR. PCR product purified by fiber matrix column and counted by gamma counter. PCR products were electrophoresized, and autoradiography image obtained. Amplified HCV DNA by {sup 125}I-dUTP PCR obtained the band on the gel by electrophoresis and autoradiography at the same position. In patient sera, radioactivity of HCV positive sample was 8 times higher than HCV negative viremia sample. We established HCV detection method using {sup 125}I-dUTP. {sup 125}I-dUTP PCR detection of HCV is convenient and reporducible.

  10. Clofarabine 5'-di and -triphosphates inhibit human ribonucleotide reductase by altering the quaternary structure of its large subunit. (United States)

    Aye, Yimon; Stubbe, Joanne


    Human ribonucleotide reductases (hRNRs) catalyze the conversion of nucleotides to deoxynucleotides and are composed of α- and β-subunits that form active α(n)β(m) (n, m = 2 or 6) complexes. α binds NDP substrates (CDP, UDP, ADP, and GDP, C site) as well as ATP and dNTPs (dATP, dGTP, TTP) allosteric effectors that control enzyme activity (A site) and substrate specificity (S site). Clofarabine (ClF), an adenosine analog, is used in the treatment of refractory leukemias. Its mode of cytotoxicity is thought to be associated in part with the triphosphate functioning as an allosteric inhibitor of hRNR. Studies on the mechanism of inhibition of hRNR by ClF di- and triphosphates (ClFDP and ClFTP) are presented. ClFTP is a reversible inhibitor (K(i) = 40 nM) that rapidly inactivates hRNR. However, with time, 50% of the activity is recovered. D57N-α, a mutant with an altered A site, prevents inhibition by ClFTP, suggesting its A site binding. ClFDP is a slow-binding, reversible inhibitor ( K(i)*; t(1/2) = 23 min). CDP protects α from its inhibition. The altered off-rate of ClFDP from E•ClFDP* by ClFTP (A site) or dGTP (S site) and its inhibition of D57N-α together implicate its C site binding. Size exclusion chromatography of hRNR or α alone with ClFDP or ClFTP, ± ATP or dGTP, reveals in each case that α forms a kinetically stable hexameric state. This is the first example of hexamerization of α induced by an NDP analog that reversibly binds at the active site.

  11. Plaque retention by self-ligating vs elastomeric orthodontic brackets: quantitative comparison of oral bacteria and detection with adenosine triphosphate-driven bioluminescence.

    NARCIS (Netherlands)

    Pellegrini, P.; Sauerwein, R.W.; Finlayson, T.; McLeod, J.; Covell, D.A.; Maier, T.; Machida, C.A.


    INTRODUCTION: Enamel decalcification is a common problem in orthodontics. The objectives of this randomized clinical study were to enumerate and compare plaque bacteria surrounding 2 bracket types, self-ligating (SL) vs elastomeric ligating (E), and to determine whether adenosine triphosphate (ATP)-

  12. Adenosine 5 '-triphosphate (ATP) supplements are not orally bioavailable: a randomized, placebo-controlled cross-over trial in healthy humans

    NARCIS (Netherlands)

    Arts, I.C.W.; Coolen, E.J.C.M.; Bours, M.J.L.; Huyghebaert, N.; Cohen Stuart, M.A.; Bast, A.; Dagnelie, P.C.


    Background: Nutritional supplements designed to increase adenosine 5'-triphosphate (ATP) concentrations are commonly used by athletes as ergogenic aids. ATP is the primary source of energy for the cells, and supplementation may enhance the ability to maintain high ATP turnover during high-intensity


    NARCIS (Netherlands)



    An Escherichia coli mutant lacking deoxycytidine triphosphate deaminase (Dcd) activity and an unknown function encoded by a gene designated ior exhibits sensitivity to ionizing radiation whereas dcd mutants themselves are not sensitive. A DNA fragment from an E. coli genomic library that restores th

  14. An improved red blood cell additive solution maintains 2,3-diphosphoglycerate and adenosine triphosphate levels by an enhancing effect on phosphofructokinase activity during cold storage

    NARCIS (Netherlands)

    P. Burger; H. Korsten; D. de Korte; E. Rombout; R. van Bruggen; A.J. Verhoeven


    BACKGROUND: Current additive solutions (ASs) for red blood cells (RBCs) do not maintain constant 2,3-diphosphoglycerate (DPG) and adenosine triphosphate (ATP) levels during cold storage We have previously shown that with a new AS called phosphate-adenine-glucose-guanosine-gluconate-mannitol (PAGGGM)

  15. Sterol transporter adenosine triphosphate-binding cassette transporter G8, gallstones, and biliary cancer in 62,000 individuals from the general population

    DEFF Research Database (Denmark)

    Stender, Stefan; Frikke-Schmidt, Ruth; Nordestgaard, Børge G;


    Gallstone disease, a risk factor for biliary cancer, has a strong heritable component, but the underlying genes are largely unknown. To test the hypothesis that ABCG8 (adenosine triphosphate-binding cassette transporter G8) Asp19His (D19H) genotype predicted risk of gallstones and biliary cancer ...

  16. Single-contact tunneling thermometry

    Energy Technology Data Exchange (ETDEWEB)

    Maksymovych, Petro


    A single-contact tunneling thermometry circuit includes a tunnel junction formed between two objects. Junction temperature gradient information is determined based on a mathematical relationship between a target alternating voltage applied across the junction and the junction temperature gradient. Total voltage measured across the junction indicates the magnitude of the target alternating voltage. A thermal gradient is induced across the junction. A reference thermovoltage is measured when zero alternating voltage is applied across the junction. An increasing alternating voltage is applied while measuring a thermovoltage component and a DC rectification voltage component created by the applied alternating voltage. The target alternating voltage is reached when the thermovoltage is nullified or doubled by the DC rectification voltage depending on the sign of the reference thermovoltage. Thermoelectric current and current measurements may be utilized in place of the thermovoltage and voltage measurements. The system may be automated with a feedback loop.

  17. Tunneling magnetoresistance in Si nanowires (United States)

    Montes, E.; Rungger, I.; Sanvito, S.; Schwingenschlögl, U.


    We investigate the tunneling magnetoresistance of small diameter semiconducting Si nanowires attached to ferromagnetic Fe electrodes, using first principles density functional theory combined with the non-equilibrium Green’s functions method for quantum transport. Silicon nanowires represent an interesting platform for spin devices. They are compatible with mature silicon technology and their intrinsic electronic properties can be controlled by modifying the diameter and length. Here we systematically study the spin transport properties for neutral nanowires and both n and p doping conditions. We find a substantial low bias magnetoresistance for the neutral case, which halves for an applied voltage of about 0.35 V and persists up to 1 V. Doping in general decreases the magnetoresistance, as soon as the conductance is no longer dominated by tunneling.

  18. Seismic scanning tunneling macroscope - Theory

    KAUST Repository

    Schuster, Gerard T.


    We propose a seismic scanning tunneling macroscope (SSTM) that can detect the presence of sub-wavelength scatterers in the near-field of either the source or the receivers. Analytic formulas for the time reverse mirror (TRM) profile associated with a single scatterer model show that the spatial resolution limit to be, unlike the Abbe limit of λ/2, independent of wavelength and linearly proportional to the source-scatterer separation as long as the point scatterer is in the near-field region; if the sub-wavelength scatterer is a spherical impedance discontinuity then the resolution will also be limited by the radius of the sphere. Therefore, superresolution imaging can be achieved as the scatterer approaches the source. This is analogous to an optical scanning tunneling microscope that has sub-wavelength resolution. Scaled to seismic frequencies, it is theoretically possible to extract 100 Hz information from 20 Hz data by imaging of near-field seismic energy.

  19. Tunneling magnetoresistance in Si nanowires

    KAUST Repository

    Montes Muñoz, Enrique


    We investigate the tunneling magnetoresistance of small diameter semiconducting Si nanowires attached to ferromagnetic Fe electrodes, using first principles density functional theory combined with the non-equilibrium Green\\'s functions method for quantum transport. Silicon nanowires represent an interesting platform for spin devices. They are compatible with mature silicon technology and their intrinsic electronic properties can be controlled by modifying the diameter and length. Here we systematically study the spin transport properties for neutral nanowires and both n and p doping conditions. We find a substantial low bias magnetoresistance for the neutral case, which halves for an applied voltage of about 0.35 V and persists up to 1 V. Doping in general decreases the magnetoresistance, as soon as the conductance is no longer dominated by tunneling.

  20. Dissipative Effect and Tunneling Time

    Directory of Open Access Journals (Sweden)

    Samyadeb Bhattacharya


    Full Text Available The quantum Langevin equation has been studied for dissipative system using the approach of Ford et al. Here, we have considered the inverted harmonic oscillator potential and calculated the effect of dissipation on tunneling time, group delay, and the self-interference term. A critical value of the friction coefficient has been determined for which the self-interference term vanishes. This approach sheds new light on understanding the ion transport at nanoscale.

  1. Variable density turbulence tunnel facility (United States)

    Bodenschatz, E.; Bewley, G. P.; Nobach, H.; Sinhuber, M.; Xu, H.


    The Variable Density Turbulence Tunnel at the Max Planck Institute for Dynamics and Self-Organization in Göttingen, Germany, produces very high turbulence levels at moderate flow velocities, low power consumption, and adjustable kinematic viscosity between 10-4 m2/s and 10-7 m2/s. The Reynolds number can be varied by changing the pressure or flow rate of the gas or by using different non-flammable gases including air. The highest kinematic viscosities, and hence lowest Reynolds numbers, are reached with air or nitrogen at 0.1 bar. To reach the highest Reynolds numbers the tunnel is pressurized to 15 bars with the dense gas sulfur hexafluoride (SF6). Turbulence is generated at the upstream ends of two measurement sections with grids, and the evolution of this turbulence is observed as it moves down the length of the sections. We describe the instrumentation presently in operation, which consists of the tunnel itself, classical grid turbulence generators, and state-of-the-art nano-fabricated hot-wire anemometers provided by Princeton University [M. Vallikivi, M. Hultmark, S. C. C. Bailey, and A. J. Smits, Exp. Fluids 51, 1521 (2011)]. We report measurements of the characteristic scales of the flow and of turbulent spectra up to Taylor Reynolds number Rλ ≈ 1600, higher than any other grid-turbulence experiment. We also describe instrumentation under development, which includes an active grid and a Lagrangian particle tracking system that moves down the length of the tunnel with the mean flow. In this configuration, the properties of the turbulence are adjustable and its structure is resolvable up to Rλ ≈ 8000.

  2. Digging the CNGS decay tunnel

    CERN Multimedia

    Patrice Loiez


    Products of the collision between a proton beam and a graphite target will pass through a horn containing an electric field that will produce a focused beam. These particles will decay into muon neutrinos within the tunnel that is being constructed in these images. The neutrinos will then travel 730 km to Gran Sasso in Italy where huge detectors will observe the beam to study a process called neutrino oscillation.

  3. Electrodiagnosis of carpal tunnel syndrome. (United States)

    Wang, Leilei


    This article discusses the historical aspects related to the understanding of carpal tunnel syndrome (CTS) and its diagnosis, highlighting observations about this disease that have yet to be challenged. This is followed by a discussion regarding the use of electrodiagnostic testing as a diagnostic tool for CTS, as well as the author's approach to making the diagnosis of CTS. Finally, conclusions about future directions in the diagnosis and treatment of this disorder are presented.

  4. Variable Density Turbulence Tunnel Facility

    CERN Document Server

    Bewley, Gregory P; Sinhuber, Michael; Xu, Haitao; Bodenschatz, Eberhard


    The Variable Density Turbulence Tunnel (VDTT) at the Max Planck Institute for Dynamics and Self-Organization in G\\"ottingen, Germany produces very high turbulence levels at moderate flow velocities, low power consumption and adjustable kinematic viscosity. To reach the highest Reynolds number, the tunnel can be filled and pressurized up to 15 bar with the dense gas sulfur hexafluoride (SF$_6$). The Reynolds number can be varied by changing the pressure or flow rate of the gas or by using different non-flammable gases including air. Turbulence is generated at the upstream ends of two measurement sections with grids, and the evolution of this turbulence is observed as it moves down the length of the sections. We describe the instrumentation presently in operation, which consists of the tunnel itself, classical grid turbulence generators, and state-of-the-art nano-fabricated hot-wire anemometers provided by Princeton University [Vallikivi et al. (2011) Exp. Fluids 51, 1521]. We report measurements of the charact...

  5. Autonomous Robotic Inspection in Tunnels (United States)

    Protopapadakis, E.; Stentoumis, C.; Doulamis, N.; Doulamis, A.; Loupos, K.; Makantasis, K.; Kopsiaftis, G.; Amditis, A.


    In this paper, an automatic robotic inspector for tunnel assessment is presented. The proposed platform is able to autonomously navigate within the civil infrastructures, grab stereo images and process/analyse them, in order to identify defect types. At first, there is the crack detection via deep learning approaches. Then, a detailed 3D model of the cracked area is created, utilizing photogrammetric methods. Finally, a laser profiling of the tunnel's lining, for a narrow region close to detected crack is performed; allowing for the deduction of potential deformations. The robotic platform consists of an autonomous mobile vehicle; a crane arm, guided by the computer vision-based crack detector, carrying ultrasound sensors, the stereo cameras and the laser scanner. Visual inspection is based on convolutional neural networks, which support the creation of high-level discriminative features for complex non-linear pattern classification. Then, real-time 3D information is accurately calculated and the crack position and orientation is passed to the robotic platform. The entire system has been evaluated in railway and road tunnels, i.e. in Egnatia Highway and London underground infrastructure.

  6. Quantum Tunneling Affects Engine Performance. (United States)

    Som, Sibendu; Liu, Wei; Zhou, Dingyu D Y; Magnotti, Gina M; Sivaramakrishnan, Raghu; Longman, Douglas E; Skodje, Rex T; Davis, Michael J


    We study the role of individual reaction rates on engine performance, with an emphasis on the contribution of quantum tunneling. It is demonstrated that the effect of quantum tunneling corrections for the reaction HO2 + HO2 = H2O2 + O2 can have a noticeable impact on the performance of a high-fidelity model of a compression-ignition (e.g., diesel) engine, and that an accurate prediction of ignition delay time for the engine model requires an accurate estimation of the tunneling correction for this reaction. The three-dimensional model includes detailed descriptions of the chemistry of a surrogate for a biodiesel fuel, as well as all the features of the engine, such as the liquid fuel spray and turbulence. This study is part of a larger investigation of how the features of the dynamics and potential energy surfaces of key reactions, as well as their reaction rate uncertainties, affect engine performance, and results in these directions are also presented here.

  7. Confocal Annular Josephson Tunnel Junctions (United States)

    Monaco, Roberto


    The physics of Josephson tunnel junctions drastically depends on their geometrical configurations and here we show that also tiny geometrical details play a determinant role. More specifically, we develop the theory of short and long annular Josephson tunnel junctions delimited by two confocal ellipses. The behavior of a circular annular Josephson tunnel junction is then seen to be simply a special case of the above result. For junctions having a normalized perimeter less than one, the threshold curves in the presence of an in-plane magnetic field of arbitrary orientations are derived and computed even in the case with trapped Josephson vortices. For longer junctions, a numerical analysis is carried out after the derivation of the appropriate motion equation for the Josephson phase. We found that the system is modeled by a modified and perturbed sine-Gordon equation with a space-dependent effective Josephson penetration length inversely proportional to the local junction width. Both the fluxon statics and dynamics are deeply affected by the non-uniform annulus width. Static zero-field multiple-fluxon solutions exist even in the presence of a large bias current. The tangential velocity of a traveling fluxon is not determined by the balance between the driving and drag forces due to the dissipative losses. Furthermore, the fluxon motion is characterized by a strong radial inward acceleration which causes electromagnetic radiation concentrated at the ellipse equatorial points.

  8. Pharmacotherapy of carpal tunnel syndrome. (United States)

    de Pablo, Paola; Katz, Jeffrey N


    Carpal tunnel syndrome (CTS) is the most common peripheral nerve entrapment syndrome. CTS is a compression neuropathy caused by elevated pressure in the carpal tunnel. CTS has the potential to substantially limit performance of activities of daily living for some individuals. The goal of therapy for CTS is to improve symptoms and reduce signs of the disease, as well as prevent progression and loss of hand function. There are several treatment alternatives to relieve the pressure on the median nerve, both surgical and conservative. The most common measures employed in the initial treatment of CTS are NSAIDs, local and systemic corticosteroids, diuretics and pyridoxine. However, CTS treatment usually includes a combination of pharmacotherapy with other strategies such as splinting and activity modification. Injections of corticosteroids into the carpal tunnel are often employed for cases not responding to conservative treatment. Surgery is superior to conservative therapies for most persistently symptomatic patients. The aim of this paper is to review the pharmacological agents used for relieving the symptoms of CTS.

  9. Enhancement of bacterial denitrification for nitrate removal in groundwater with electrical stimulation from microbial fuel cells (United States)

    Zhang, Baogang; Liu, Ye; Tong, Shuang; Zheng, Maosheng; Zhao, Yinxin; Tian, Caixing; Liu, Hengyuan; Feng, Chuanping


    Electricity generated from the microbial fuel cell (MFC) is applied to the bioelectrical reactor (BER) directly as electrical stimulation means for enhancement of bacterial denitrification to remove nitrate effectively from groundwater. With maximum power density of 502.5 mW m-2 and voltage outputs ranging from 500 mV to 700 mV, the nitrate removal is accelerated, with less intermediates accumulation, compared with control sets without electrical stimulation. Denitrification bacteria proliferations and activities are promoted as its number and Adenosine-5'-triphosphate (ATP) concentration increased one order of magnitude (3.5 × 107 in per milliliter biofilm solution) and about 1.5 folds, respectively. Effects of electricity from MFCs on enhancement of bacterial behaviors are demonstrated for the first time. These results indicate that MFCs can be applied in the in-situ bioremediation of nitrate polluted groundwater for efficiency improvement.

  10. Investigation into scanning tunnelling luminescence microscopy

    CERN Document Server

    Manson-Smith, S K


    This work reports on the development of a scanning tunnelling luminescence (STL) microscope and its application to the study of Ill-nitride semiconductor materials used in the production of light emitting devices. STL microscopy is a technique which uses the high resolution topographic imaging capabilities of the scanning tunnelling microscope (STM) to generate high resolution luminescence images. The STM tunnelling current acts as a highly localised source of electrons (or holes) which generates luminescence in certain materials. Light generated at the STM tunnelling junction is collected concurrently with the height variation of the tunnelling probe as it is scanned across a sample surface, producing simultaneous topographic and luminescence images. Due to the very localised excitation source, high resolution luminescence images can be obtained. Spectroscopic resolution can be obtained by using filters. Additionally, the variation of luminescence intensity with tunnel current and with bias voltage can provi...

  11. Tunneling Dynamics Between Atomic Bright Solitons

    CERN Document Server

    Zhao, Li-Chen; Yang, Zhan-Ying; Yang, Wen-Li


    We investigate tunneling behavior between two bright solitons in a Bose-Einstein condensate with attractive contact interactions between atoms. The explicit tunneling properties including tunneling particles and oscillation period are described analytically, which indicates that the periodic tunneling form is a nonlinear Josephson type oscillation. The results suggest that the breathing behavior of solitons comes from the tunneling mechanism in an effective double-well potential, which is quite different from the modulational instability mechanism for Akhmediev breather and K-M breather. Furthermore, we obtain a phase diagram for two soliton interaction which admits tunneling property, particle-like property, interference property, and a resonant interaction case. The explicit conditions for them are clarified based on the defined critical distance $d_c$ and spatial interference period $D$.

  12. Tunnel design considering stress release effect

    Institute of Scientific and Technical Information of China (English)

    Van-hung DAO


    In tunnel design,the determination of installation time and the stiffness of supporting structures is very important to the tunnel stability.This study used the convergence-confinement method to determine the stress and displacement of the tunnel while considering the counter-pressure curve of the ground base,the stress release effect,and the interaction between the tunnel lining and the rock surrounding the tunnel chamber.The results allowed for the determination of the installation time,distribution and strength of supporting structures.This method was applied to the intake tunnel in the Ban Ve Hydroelectric Power Plant,in Nghe An Province,Vietnam.The results show that when a suitable displacement u0 ranging from 0.0865 m to 0.0919 m occurrs,we can install supporting structures that satisfy the stability and economical requirements.

  13. RITD – Wind tunnel testing (United States)

    Haukka, Harri; Harri, Ari-Matti; Aleksashkin, Sergei; Koryanov, Valeri; Schmidt, Walter; Heilimo, Jyri; Finchenko, Valeri; Martynov, Maxim; Ponomarenko, Andrey; Kazakovtsev, Victor; Arruego, Ignazio


    An atmospheric re-entry and descent and landing system (EDLS) concept based on inflatable hypersonic decelerator techniques is highly promising for the Earth re-entry missions. We developed such EDLS for the Earth re-entry utilizing a concept that was originally developed for Mars. This EU-funded project is called RITD - Re-entry: Inflatable Technology Development - and it was to assess the bene¬fits of this technology when deploying small payloads from low Earth orbits to the surface of the Earth with modest costs. The principal goal was to assess and develope a preliminary EDLS design for the entire relevant range of aerodynamic regimes expected to be encountered in Earth's atmosphere during entry, descent and landing. The RITD entry and descent system utilizes an inflatable hypersonic decelerator. Development of such system requires a combination of wind tunnel tests and numerical simulations. This included wind tunnel tests both in transsonic and subsonic regimes. The principal aim of the wind tunnel tests was the determination of the RITD damping factors in the Earth atmosphere and recalculation of the results for the case of the vehicle descent in the Mars atmosphere. The RITD mock-up model used in the tests was in scale of 1:15 of the real-size vehicle as the dimensions were (midsection) diameter of 74.2 mm and length of 42 mm. For wind tunnel testing purposes the frontal part of the mock-up model body was manufactured by using a PolyJet 3D printing technology based on the light curing of liquid resin. The tail part of the mock-up model body was manufactured of M1 grade copper. The structure of the mock-up model placed th center of gravity in the same position as that of the real-size RITD. The wind tunnel test program included the defining of the damping factor at seven values of Mach numbers 0.85; 0.95; 1.10; 1.20; 1.25; 1.30 and 1.55 with the angle of attack ranging from 0 degree to 40 degrees with the step of 5 degrees. The damping characteristics of

  14. Extended recombinant bacterial ghost system. (United States)

    Lubitz, W; Witte, A; Eko, F O; Kamal, M; Jechlinger, W; Brand, E; Marchart, J; Haidinger, W; Huter, V; Felnerova, D; Stralis-Alves, N; Lechleitner, S; Melzer, H; Szostak, M P; Resch, S; Mader, H; Kuen, B; Mayr, B; Mayrhofer, P; Geretschläger, R; Haslberger, A; Hensel, A


    Controlled expression of cloned PhiX174 gene E in Gram-negative bacteria results in lysis of the bacteria by formation of an E-specific transmembrane tunnel structure built through the cell envelope complex. Bacterial ghosts from a variety of bacteria are used as non-living candidate vaccines. In the recombinant ghost system, foreign proteins are attached on the inside of the inner membrane as fusions with specific anchor sequences. Ghosts have a sealed periplasmic space and the export of proteins into this space vastly extends the capacity of ghosts or recombinant ghosts to function as carriers of foreign antigens. In addition, S-layer proteins forming shell-like self assembly structures can be expressed in candidate vaccine strains prior to E-mediated lysis. Such recombinant S-layer proteins carrying foreign epitopes further extend the possibilities of ghosts as carriers of foreign epitopes. As ghosts have inherent adjuvant properties, they can be used as adjuvants in combination with subunit vaccines. Subunits or other ligands can also be coupled to matrixes like dextran which are used to fill the internal lumen of ghosts. Oral, aerogenic or parenteral immunization of experimental animals with recombinant ghosts induced specific humoral and cellular immune responses against bacterial and target components including protective mucosal immunity. The most relevant advantage of recombinant bacterial ghosts as immunogens is that no inactivation procedures that denature relevant immunogenic determinants are employed in this production. This fact explains the superior quality of ghosts when compared to other inactivated vaccines. The endotoxic component of the outer membrane does not limit the use of ghosts as vaccine candidates but triggers the release of several potent immunoregulatory cytokines. As carriers, there is no limitation in the size of foreign antigens that can be inserted in the membrane and the capacity of all spaces including the membranes, peri

  15. Quantum tunneling and field electron emission theories

    CERN Document Server

    Liang, Shi-Dong


    Quantum tunneling is an essential issue in quantum physics. Especially, the rapid development of nanotechnology in recent years promises a lot of applications in condensed matter physics, surface science and nanodevices, which are growing interests in fundamental issues, computational techniques and potential applications of quantum tunneling. The book involves two relevant topics. One is quantum tunneling theory in condensed matter physics, including the basic concepts and methods, especially for recent developments in mesoscopic physics and computational formulation. The second part is the f

  16. Raynaud's syndrome and carpal tunnel syndrome.


    Waller, D G; Dathan, J R


    We report three cases of Raynaud's syndrome with digital ischaemic ulceration, in association with carpal tunnel syndrome. In all cases, the aetiology of the Raynaud's syndrome was probably unrelated to the nerve compression. However, symptoms were worse on the side of the median nerve lesion in two patients and worse on the side with the most severe nerve dysfunction in the third; symptoms were relieved by carpal tunnel decompression in two patients. We suggest that carpal tunnel syndrome ma...



    ERKAN, İbrahim Hakkı; Yılmaz, Ülkü Sultan; Türken, Hakan


    Tunnel formwork systems became the most widespread construction technology preferred in recent years in Turkey due to rapidity and economy. In this study, before all else, a brief information was given about the types and construction techniques of tunnel formwork systems. Then a reinforced concrete building sample was firstly modeled as it was constructed with the tunnel formwork system and secondly modeled as it was formed by a frame system with shear walls constructed by the traditional re...

  18. Electromyographic diagnosis of the carpal tunnel syndrome

    Directory of Open Access Journals (Sweden)

    K. Toyonaga


    Full Text Available Sensory conduction velocities of the median nerVe were studied from digit to palm and from palm to wrist in normal subjects and in patients with the carpal tunnel syndrome. Definite slowing was noted in the palm to wrist segment, even in the early carpal tunnel syndrome. It was noted that 37% of normal women over 40 years of age had electrophysiological evidence of the carpal tunnel syndrome.

  19. Low-Frequency Electromagnetic Backscattering from Tunnels

    Energy Technology Data Exchange (ETDEWEB)

    Casey, K; Pao, H


    Low-frequency electromagnetic scattering from one or more tunnels in a lossy dielectric half-space is considered. The tunnel radii are assumed small compared to the wavelength of the electromagnetic field in the surrounding medium; a tunnel can thus be modeled as a thin scatterer, described by an equivalent impedance per unit length. We examine the normalized backscattering width for cases in which the air-ground interface is either smooth or rough.

  20. Peritonitis - spontaneous bacterial (United States)

    Spontaneous bacterial peritonitis (SBP); Ascites - peritonitis; Cirrhosis - peritonitis ... who are on peritoneal dialysis for kidney failure. Peritonitis may have other causes . These include infection from ...

  1. Predictive modelling of ferroelectric tunnel junctions (United States)

    Velev, Julian P.; Burton, John D.; Zhuravlev, Mikhail Ye; Tsymbal, Evgeny Y.


    Ferroelectric tunnel junctions combine the phenomena of quantum-mechanical tunnelling and switchable spontaneous polarisation of a nanometre-thick ferroelectric film into novel device functionality. Switching the ferroelectric barrier polarisation direction produces a sizable change in resistance of the junction—a phenomenon known as the tunnelling electroresistance effect. From a fundamental perspective, ferroelectric tunnel junctions and their version with ferromagnetic electrodes, i.e., multiferroic tunnel junctions, are testbeds for studying the underlying mechanisms of tunnelling electroresistance as well as the interplay between electric and magnetic degrees of freedom and their effect on transport. From a practical perspective, ferroelectric tunnel junctions hold promise for disruptive device applications. In a very short time, they have traversed the path from basic model predictions to prototypes for novel non-volatile ferroelectric random access memories with non-destructive readout. This remarkable progress is to a large extent driven by a productive cycle of predictive modelling and innovative experimental effort. In this review article, we outline the development of the ferroelectric tunnel junction concept and the role of theoretical modelling in guiding experimental work. We discuss a wide range of physical phenomena that control the functional properties of ferroelectric tunnel junctions and summarise the state-of-the-art achievements in the field.

  2. Femtosecond tunneling response of surface plasmon polaritons

    DEFF Research Database (Denmark)

    Keil, Ulrich Dieter Felix; Ha, Taekjip; Jensen, Jacob Riis;


    We obtain femtosecond (200 fs) time resolution using a scanning tunneling microscope on surface plasmon polaritons (SPPs) generated by two 100 fs laser beams in total internal reflection geometry. The tunneling gap dependence of the signal clearly indicates the tunneling origin of the signal...... and suggests that nanometer spatial resolution can be obtained together with femtosecond temporal resolution. This fast response, in contrast to the picosecond decay time of SPPs revealed by differential reflectivity measurements, can be attributed to a coherent superposition of SPPs rectified at the tunneling...

  3. Fire safety assessment of tunnel structures

    DEFF Research Database (Denmark)

    Gkoumas, Konstantinos; Giuliani, Luisa; Petrini, Francesco


    durability provisions, commitment to environmental aspects, issues of sustainability and safety assurance, for their whole lifecycle. The design for safety of tunnel infrastructures is a multifaceted process, since there are many aspects that need to be accounted for, regarding different aspects (e...... for upgrading fire safety provisions and tunnel management are also important for existing tunnels. In this study, following a brief introduction of issues regarding the above mentioned aspects, the structural performance of a steel rib for a tunnel infrastructure subject to fire is assessed by means...

  4. Surgical efficacy of carpal tunnel release for carpal tunnel syndrome in acromegaly: report of four patients. (United States)

    Iwasaki, N; Masuko, T; Ishikawa, J; Minami, A


    Although carpal tunnel syndrome is frequent in acromegaly, few acromegalics will be encountered by most hand surgeons. This paper considers the treatment of four cases of acromegaly in whom carpal tunnel syndrome arose, to discuss aspects of management of carpal tunnel syndrome in this patient group.

  5. Tunneling electroresistance of MgZnO-based tunnel junctions (United States)

    Belmoubarik, Mohamed; Al-Mahdawi, Muftah; Obata, Masao; Yoshikawa, Daiki; Sato, Hideyuki; Nozaki, Tomohiro; Oda, Tatsuki; Sahashi, Masashi


    We investigated the tunneling electroresistance (TER) in metal/wurtzite-MgZnO/metal junctions for applications in nonvolatile random-access memories. A resistive switching was detected utilizing an electric-field cooling at ±1 V and exhibited a TER ratio of 360%-490% at 2 K. The extracted change in the average barrier height between the two resistance states gave an estimation of the MgZnO electric polarization at 2.5 μC/cm2 for the low-temperature limit. In addition, the temperature-dependent TER ratio and the shift of the localized states energies at the barrier interface supported the ferroelectric behavior of the MgZnO tunnel-barrier. From the first-principles calculations, we found a similar effect of the barrier height change coming from the reversal of ZnO electric polarization. The possibility of using metal electrodes and lower growth temperatures, in addition to the ferroelectric property, make the ZnO-based memory devices suitable for CMOS integration.

  6. More about tunnelling times and superluminal tunnelling (Hartmann effect)

    Energy Technology Data Exchange (ETDEWEB)

    Olkhovsky, V.S. [Ukrainian Akademy of Sciences, Kiev (Ukraine). Inst. for Nuclear Research]|[INFN-Sezione di Catania (Italy); Recami, E. [Bergamo Univ. (Italy). Facolta` di Ingegneria]|[State Univ. at Campinas, Campinas (Brazil); Raciti, F. [Catania Univ. (Italy); Zaichenko, A. [Ukrainian Akademy of Sciences, Kiev (Ukraine). Inst. for Nuclear Reserch


    Aims of the present paper are: (i) presenting and analysing the results of various numerical calculations on the penetration and return times <{tau}{sub Pen}>, <{tau}{sub Ret}>, during tunnelling inside a rectangular potential barrier, for various penetration depths x{sub f}; (ii) putting forth and discussing suitable definitions, besides of the mean values, also of the variances (or dispersions) D{sub {tau}T} and D{sub {tau}R} for the time durations of transmission and reflection processes; (iii) mentioning, moreover, that our definition <{tau}{sub T}> for the average transmission time results to constitute an improvement of the ordinary dwell- time formula; (iv) commenting, at last, on the basis of the new numerical results, upon some recent criticism by C.R. Leavens. The paper stresses that numerical evaluations confirm that the approach implied, and implies, the existence of the Hartmann effect: an effect that in these days (due to the theoretical connections between tunnelling and evanescent-wave propagation) is receiving - at Cologne, Berkeley, Florence and Vienna - indirect, but quite interesting, experimental verification.

  7. Metal-Vacuum-Metal Tunneling (United States)


    the unexposed areas was removed with wet etchants. The Al lines formed in this way were 1000 A in width. The field emission mode was also used by...positioned close to the substrate within tunneling range. Whey they ramped the tip voltage to 10-20 volts they formed patterns on the substrate that were...associated with the light induced decomposition of III-V compounds. It is based on well-known work on photo- oxidation and the anisotropic etching of III-V

  8. Wall Interference in Wind Tunnels (United States)


    coefficient de trainee de pression coefficient de trainee de frottement coefficient de trainee totale (sillage) nombre de Mach ei M pression...correction by classical methods. | j SYMBOLS I j I b tunnel breadth I c aerofoil chord I Cn drag coefficient , referred to äpeUe 2c j Cm... coefficient of pitching moment about leading edge, nose up positive, referred j to JpeUe 2c2 L, i: CN normal-force coefficient , referred to ipeUg c | i Cp

  9. Tunneling without barriers with gravity

    CERN Document Server

    Kanno, Sugumi; Soda, Jiro


    We consider the vacuum decay of the flat Minkowski space to an anti-de Sitter space. We find a one-parameter family of potentials that allow exact, analytical instanton solutions describing tunneling without barriers in the presence of gravity. In the absence of gravity such instantons were found and discussed by Lee and Weinberg more than a quarter of a century ago. The bounce action is also analytically computed. We discuss possible implications of these new instantons to cosmology in the context of the string theory landscape.

  10. Full-Scale Tunnel (FST) (United States)


    Modified propeller and spinner in Full-Scale Tunnel (FST) model. On June 26, 1929, Elton W. Miller wrote to George W. Lewis proposing the construction of a model of the full-scale tunnel. 'The excellent energy ratio obtained in the new wind tunnel of the California Institute of Technology suggests that before proceeding with our full scale tunnel design, we ought to investigate the effect on energy ratio of such factors as: 1. small included angle for the exit cone; 2. carefully designed return passages of circular section as far as possible, without sudden changes in cross sections; 3. tightness of walls. It is believed that much useful information can be obtained by building a model of about 1/16 scale, that is, having a closed throat of 2 ft. by 4 ft. The outside dimensions would be about 12 ft. by 25 ft. in plan and the height 4 ft. Two propellers will be required about 28 in. in diameter, each to be driven by direct current motor at a maximum speed of 4500 R.P.M. Provision can be made for altering the length of certain portions, particularly the exit cone, and possibly for the application of boundary layer control in order to effect satisfactory air flow. This model can be constructed in a comparatively short time, using 2 by 4 framing with matched sheathing inside, and where circular sections are desired they can be obtained by nailing sheet metal to wooden ribs, which can be cut on the band saw. It is estimated that three months will be required for the construction and testing of such a model and that the cost will be approximately three thousand dollars, one thousand dollars of which will be for the motors. No suitable location appears to exist in any of our present buildings, and it may be necessary to build it outside and cover it with a roof.' George Lewis responded immediately (June 27) granting the authority to proceed. He urged Langley to expedite construction and to employ extra carpenters if necessary. Funds for the model came from the FST project

  11. Quantum tunnelling in condensed media

    CERN Document Server

    Kagan, Yu


    The essays in this book deal with of the problem of quantum tunnelling and related behavior of a microscopic or macroscopic system, which interacts strongly with an ""environment"" - this being some form of condensed matter. The ""system"" in question need not be physically distinct from its environment, but could, for example, be one particular degree of freedom on which attention is focussed, as in the case of the Josephson junction studied in several of the papers. This general problem has been studied in many hundreds, if not thousands, of articles in the literature, in contexts as diverse

  12. Dynamical tunneling in optical cavities

    CERN Document Server

    Hackenbroich, G; Hackenbroich, Gregor; Noeckel, Jens U.


    The lifetime of whispering gallery modes in a dielectric cavity with a metallic inclusion is shown to fluctuate by orders of magnitude when size and location of the inclusion are varied. We ascribe these fluctuations to tunneling transitions between resonances quantized in different regions of phase space. This interpretation is confirmed by a comparison of the classical phase space structure with the Husimi distribution of the resonant modes. A model Hamiltonian is introduced that describes the phenomenon and shows that it can be expected in a more general class of systems.

  13. Evidence for a "metabolically inactive" inorganic phosphate pool in adenosine triphosphate synthase reaction using localized 31P saturation transfer magnetic resonance spectroscopy in the rat brain at 11.7 T. (United States)

    Tiret, Brice; Brouillet, Emmanuel; Valette, Julien


    With the increased spectral resolution made possible at high fields, a second, smaller inorganic phosphate resonance can be resolved on (31)P magnetic resonance spectra in the rat brain. Saturation transfer was used to estimate de novo adenosine triphosphate synthesis reaction rate. While the main inorganic phosphate pool is used by adenosine triphosphate synthase, the second pool is inactive for this reaction. Accounting for this new pool may not only help us understand (31)P magnetic resonance spectroscopy metabolic profiles better but also better quantify adenosine triphosphate synthesis.

  14. Prevention of bacterial adhesion

    DEFF Research Database (Denmark)

    Klemm, Per; Vejborg, Rebecca Munk; Hancock, Viktoria


    Management of bacterial infections is becoming increasingly difficult due to the emergence and increasing prevalence of bacterial pathogens that are resistant to available antibiotics. Conventional antibiotics generally kill bacteria by interfering with vital cellular functions, an approach that ...... valuable weapons for preventing pathogen contamination and fighting infectious diseases in the future....

  15. Vimentin in Bacterial Infections

    DEFF Research Database (Denmark)

    Mak, Tim N; Brüggemann, Holger


    Despite well-studied bacterial strategies to target actin to subvert the host cell cytoskeleton, thus promoting bacterial survival, replication, and dissemination, relatively little is known about the bacterial interaction with other components of the host cell cytoskeleton, including intermediate...... filaments (IFs). IFs have not only roles in maintaining the structural integrity of the cell, but they are also involved in many cellular processes including cell adhesion, immune signaling, and autophagy, processes that are important in the context of bacterial infections. Here, we summarize the knowledge...... about the role of IFs in bacterial infections, focusing on the type III IF protein vimentin. Recent studies have revealed the involvement of vimentin in host cell defenses, acting as ligand for several pattern recognition receptors of the innate immune system. Two main aspects of bacteria...

  16. Computational Wind Tunnel: A Design Tool for Rotorcraft Project (United States)

    National Aeronautics and Space Administration — Rotorcraft engineers traditionally use the wind tunnel to evaluate and finalize designs. Insufficient correlation between wind tunnel results and flight tests, have...

  17. A Supermagnetic Tunnel Full of Subatomic Action

    CERN Multimedia


    Last year, before the gigantic hadron supercollider at CERN research facility was installed underground, a photographer captured this picture of a 1,950 metric ton tunnel containing giant magnets that will be placed in a tunnel and kept at near-zero temperatures.

  18. Virtual Processes and Quantum Tunnelling as Fictions (United States)

    Arthur, Richard T. W.


    In this paper it is argued that virtual processes are dispensable fictions. The argument proceeds by a comparison with the phenomenon of quantum tunnelling. Building on an analysis of Levy-Leblond and Balibar, it is argued that, although the phenomenon known as quantum tunnelling certainly occurs and is at the basis of many paradigmatic quantum…

  19. Superconducting Super Collider Laboratory : tunnel boring

    CERN Document Server

    SSC Media Production


    This film will take you down into the tunnel, show you the technology involved in boring the tunnel, and show what the SSC fmeans to the U.S. in terms of scientific discovery, innovative collaborations with industry and stimulating the job base nation-wide.

  20. Ultrasonographic assessment of carpal tunnel biomechanics

    NARCIS (Netherlands)

    van Doesburg, M.H.M.


    In this thesis, we searched for a way to assess flexor tendon and median nerve biomechanics, as well as subsynovial connective tissue thickness (SSCT) in the carpal tunnel with ultrasound, and tried to see if these patterns would give a clue towards understanding the etiology of carpal tunnel syndro

  1. Prediction of swelling rocks strain in tunneling (United States)

    Parsapour, D.; Fahimifar, A.


    Swelling deformations leading to convergence of tunnels may result in significant difficulties during the construction, in particular for long term use of tunnels. By extracting an experimental based explicit analytical solution for formulating swelling strains as a function of time and stress, swelling strains are predicted from the beginning of excavation and during the service life of tunnel. Results obtained from the analytical model show a proper agreement with experimental results. This closed-form solution has been implemented within a numerical program using the finite element method for predicting time-dependent swelling strain around tunnels. Evaluating effects of swelling parameters on time-dependent strains and tunnel shape on swelling behavior around the tunnel according to this analytical solution is considered. The ground-support interaction and consequent swelling effect on the induced forces in tunnel lining is considered too. Effect of delay in lining installation on swelling pressure which acting on the lining and its structural integrity, is also evaluated. A MATLAB code of " SRAP" is prepared and applied to calculate all swelling analysis around tunnels based on analytical solution.

  2. Transport of dangerous goods through road tunnels

    DEFF Research Database (Denmark)

    Jørgensen, N O; Lacroix, Didier; Amundsen, F.H.;


    A paper which describes the work of an OECD research group. The group has suggested a grouping of dangerous materials, a quantitative risk assessment model and a decision support model which should allow tunnel operators to determine if a given material should be allowed throug a given tunnel...

  3. Flow-based detection of DNS tunnels

    NARCIS (Netherlands)

    Ellens, W.; Zuraniewski, P.W.; Sperotto, A.; Schotanus, H.A.; Mandjes, M.; Meeuwissen, H.B.


    DNS tunnels allow circumventing access and security policies in firewalled networks. Such a security breach can be misused for activities like free web browsing, but also for command & control traffic or cyber espionage, thus motivating the search for effective automated DNS tunnel detection techniq

  4. Colloquium: Time-resolved scanning tunneling microscopy

    NARCIS (Netherlands)

    Houselt, van Arie; Zandvliet, Harold J.W.


    Scanning tunneling microscopy has revolutionized our ability to image, study, and manipulate solid surfaces on the size scale of atoms. One important limitation of the scanning tunneling microscope (STM) is, however, its poor time resolution. Recording a standard image with a STM typically takes abo

  5. Spin-valley filter and tunnel magnetoresistance in asymmetrical silicene magnetic tunnel junctions (United States)

    Wang, Dali; Huang, Zeyuan; Zhang, Yongyou; Jin, Guojun


    The spin and valley transports and tunnel magnetoresistance are studied in a silicene-based asymmetrical magnetic tunnel junction consisting of a ferromagnetic tunnel barrier, sandwiched between a ferromagnetic electrode and a normal electrode. For such an asymmetrical silicene junction, a general formulism is established. The numerical results show that the spin-valley resolved conductances strongly depend on the magnetization orientation of the ferromagnetic tunnel barrier, and the fully spin-valley polarized current can be realized by tuning a perpendicularly applied electric field. We also find that the tunnel magnetoresistance in this case can be effectively modified by the external electric field when the conductance is fully spin-valley polarized. In particular, the exchange field in the ferromagnetic electrode can further substantially enhance the tunnel magnetoresistance of the system. Our work provides a practical method for electric and magnetic manipulation of valley/spin polarization and tunnel magnetoresistance.

  6. [Carpal tunnel syndrome. Current approaches]. (United States)

    Kouyoumdjian, J A


    A clinical, epidemiological and nerve conduction studies report on carpal tunnel syndrome was done after electrophysiological author's experience on 668 cases and literature review. The median nerve underwent focal (nodal) or segmental demyelination after compression on carpal tunnel, 3-4 distal to wrist fold. The symptomatic complex includes nocturnal hands numbness and paraesthesia, mostly bilateral and between 40-60 years old. Familial cases are described and the gene could encode thick transverse carpal ligament. Anthropomorphic findings could also bring about an additional risk, but with low significance. Magnetic resonance could be a useful tool for selected atypical cases. Conservative treatment and controversies on surgery timing are discussed. Classical conduction studies on median nerve reveal a prolonged distal segmental sensory latency and also on distal motor latency. Increasing sensitivity may be reach using additional methods such as, median mixed mid-palm latency, comparative mid-palm latency median/ulnar, comparative sensory latency median/radial and median/ulnar, inching method from wrist to palm recording on index/middle finger and comparative motor median/ulnar recording on lumbrical/interosseous muscle.

  7. Hydrogen tunneling in enzyme reactions

    Energy Technology Data Exchange (ETDEWEB)

    Cha, Y.; Murray, C.J.; Klinman, J.P.


    Primary and secondary protium-to-tritium (H/T) and deuterium-to-tritium (D/T) kinetic isotope effects for the catalytic oxidation of benzyl alcohol to benzaldehyde by yeast alcohol dehydrogenase (YADH) at 25 degrees Celsius have been determined. Previous studies showed that this reaction is nearly or fully rate limited by the hydrogen-transfer step. Semiclassical mass considerations that do not include tunneling effects would predict that kH/kT = (kD/kT)3.26, where kH, kD, and kT are the rate constants for the reaction of protium, deuterium, and tritium derivatives, respectively. Significant deviations from this relation have now been observed for both primary and especially secondary effects, such that experimental H/T ratios are much greater than those calculated from the above expression. These deviations also hold in the temperature range from 0 to 40 degrees Celsius. Such deviations were previously predicted to result from a reaction coordinate containing a significant contribution from hydrogen tunneling.

  8. Hydrogen Tunneling in Enzyme Reactions (United States)

    Cha, Yuan; Murray, Christopher J.; Klinman, Judith P.


    Primary and secondary protium-to-tritium (H/T) and deuterium-to-tritium (D/T) kinetic isotope effects for the catalytic oxidation of benzyl alcohol to benzaldehyde by yeast alcohol dehydrogenase (YADH) at 25 degrees Celsius have been determined. Previous studies showed that this reaction is nearly or fully rate limited by the hydrogen-transfer step. Semiclassical mass considerations that do not include tunneling effects would predict that kH/kT = (kD/kT)3.26, where kH, kD, and kT are the rate constants for the reaction of protium, deuterium, and tritium derivatives, respectively. Significant deviations from this relation have now been observed for both primary and especially secondary effects, such that experimental H/T ratios are much greater than those calculated from the above expression. These deviations also hold in the temperature range from 0 to 40 degrees Celsius. Such deviations were previously predicted to result from a reaction coordinate containing a significant contribution from hydrogen tunneling.

  9. Temporary closure of the tunnel

    CERN Document Server

    Relations with the Host States Service


    Owing to major maintenance work, the tunnel linking the various parts of the CERN site will be closed from Monday 4 July to Sunday 24 July 2005 The Host State authorities have given authorisation for persons employed by CERN or the Institutes to travel and for goods belonging to these entities to be transported between the various parts of the site via Gate E (Charles de Gaulle) while this work is being carried out, subject to strict compliance with the Rules for the Use of the Tunnel (see Gate E will thus be open between 7.00 a.m. and 7.00 p.m. from Monday to Friday during the period concerned. The rules governing the use of Gate E to enter the Meyrin site between 7.30 a.m. and 9.00 a.m. or to leave the site between 5.00 p.m. and 6.30 p.m. (see will remain unaffected by this temporary authorisation. Relations with the Host States Service and TS-FM Group


    Sousa, Daniel; Sassul, Nicolás


    Objectives: About a group of cyclists, professionals / amateurs, Mountain bike, road and triathlon; achieve a good diagnosis of the disease, with a good clinical examination and sectorized according EGM injury evoked potentials. Methods: Clinical examination and accurate test with different signs of pathology. EGM with evocative potential and conduction velocity. Results: After 25 track cyclists, 18 professionals, 22 male and 3 female; for 24 months. Through good clinical examination and EMG. We got that 70% had direct compression injuries Carpal tunnel for poor support on the handlebars. The rest were cervical praxis, by poor body position on the bike, taking cervico very steep angles / dorsal, during competitions or training for more than 2 hrs. Conclusion: A good prevention work with our teacher / cyclist in the position of deposrtista in ciclo simulador. Work in the gym, on tone and elongation of the upper limb. A good EGM, made with a specialist physiatrist. It leads to the correct diagnosis, leads to a good final treatment; which agreed that:* Cervical praxis, had good results with treatment Conservative / FST / vit.B12.* The Carpal tunnel own injuries, treatment was quirúrg. (Open surgery) with subsequent FST / vit..B12 with satisfactory return in time to sporting activity.

  11. Binding of Mn-deoxyribonucleoside Triphosphates to the Active Site of the DNA Polymerase of Bacteriophage T7

    Energy Technology Data Exchange (ETDEWEB)

    B Akabayov; C Richardson


    Divalent metal ions are crucial as cofactors for a variety of intracellular enzymatic activities. Mg{sup 2+}, as an example, mediates binding of deoxyribonucleoside 5'-triphosphates followed by their hydrolysis in the active site of DNA polymerase. It is difficult to study the binding of Mg{sup 2+} to an active site because Mg{sup 2+} is spectroscopically silent and Mg{sup 2+} binds with low affinity to the active site of an enzyme. Therefore, we substituted Mg{sup 2+} with Mn{sup 2+}:Mn{sup 2+} that is not only visible spectroscopically but also provides full activity of the DNA polymerase of bacteriophage T7. In order to demonstrate that the majority of Mn{sup 2+} is bound to the enzyme, we have applied site-directed titration analysis of T7 DNA polymerase using X-ray near edge spectroscopy. Here we show how X-ray near edge spectroscopy can be used to distinguish between signal originating from Mn{sup 2+} that is free in solution and Mn{sup 2+} bound to the active site of T7 DNA polymerase. This method can be applied to other enzymes that use divalent metal ions as a cofactor.

  12. Upregulation of nucleoside triphosphate diphosphohydrolase-1 and ecto-5'-nucleotidase in rat hippocampus after repeated low-dose dexamethasone administration. (United States)

    Drakulić, Dunja; Stanojlović, Miloš; Nedeljković, Nadežda; Grković, Ivana; Veličković, Nataša; Guševac, Ivana; Mitrović, Nataša; Buzadžić, Ivana; Horvat, Anica


    Although dexamethasone (DEX), a synthetic glucocorticoid receptor (GR) analog with profound effects on energy metabolism, immune system, and hypothalamic-pituitary-adrenal axis, is widely used therapeutically, its impact on the brain is poorly understood. The aim of the present study was to explore the effect of repeated low-dose DEX administration on the activity and expression of the ectonucleotidase enzymes which hydrolyze and therefore control extracellular ATP and adenosine concentrations in the synaptic cleft. Ectonucleotidases tested were ectonucleoside triphosphate diphosphohydrolase 1-3 (NTPDase1-3) and ecto-5'-nucleotidase (eN), whereas the effects were evaluated in two brain areas that show different sensitivity to glucocorticoid action, hippocampus, and cerebral cortex. In the hippocampus, but not in cerebral cortex, modest level of neurodegenerative changes as well as increase in ATP, ADP, and AMP hydrolysis and upregulation of NTPDase1 and eN mRNA expression ensued under the influence of DEX. The observed pattern of ectonucleotidase activation, which creates tissue volume with enhanced capacity for adenosine formation, is the hallmark of the response after different insults to the brain.

  13. Peroxynitrite-dependent zinc release and inactivation of guanosine 5'-triphosphate cyclohydrolase 1 instigate its ubiquitination in diabetes. (United States)

    Zhao, Yu; Wu, Jiliang; Zhu, Huaiping; Song, Ping; Zou, Ming-Hui


    Aberrant degradation of guanosine 5'-triphosphate cyclohydrolase 1 (GTPCH1) with consequent deficiency of tetrahydrobiopterin is considered the primary cause for endothelial dysfunction in diabetes. How GTPCH1 becomes susceptible to the degradation remains unknown. We hypothesized that oxidation and release of the zinc ion by peroxynitrite (ONOO(-)), a potent oxidant generated by nitric oxide and superoxide anions, instigates GTPCH1 ubiquitination and degradation. Zinc contents, GTPCH1 ubiquitination, and GTPCH1 activity were assayed in purified GTPCH1, endothelial cells, and hearts from diabetic mice. Exogenous ONOO(-) dose-dependently released zinc, inhibited its activity, and increased the ubiquitin binding affinity of GTPCH1 in vitro and in endothelial cells. Consistently, high glucose (30 mmol/L) inhibited GTPCH1 activity with increased ubiquitination, which was inhibited by antioxidants. Furthermore, mutation of the zinc-binding cysteine (141) (C141R or C141A) significantly reduced GTPCH1 activity and reduced its half-life but increased GTPCH1 ubiquitination, indicating an essential role of the zinc ion in maintaining the catalytic activity and stability of GTPCH1. Finally, GTPCH1 ubiquitination and degradation markedly increased in parallel with decreased GTPCH1 activity in the aortas and hearts of diabetic mice, both of which were attenuated by the inhibitors of ONOO(-) in mice in vivo. Taken together, we conclude that ONOO(-) releases zinc and inhibits GTPCH1, resulting in its ubiquitination and degradation of the enzyme.

  14. The crystal structure of the Leishmania major deoxyuridine triphosphate nucleotidohydrolase in complex with nucleotide analogues, dUMP, and deoxyuridine. (United States)

    Hemsworth, Glyn R; Moroz, Olga V; Fogg, Mark J; Scott, Benjamin; Bosch-Navarrete, Cristina; González-Pacanowska, Dolores; Wilson, Keith S


    Members of the Leishmania genus are the causative agents of the life-threatening disease leishmaniasis. New drugs are being sought due to increasing resistance and adverse side effects with current treatments. The knowledge that dUTPase is an essential enzyme and that the all α-helical dimeric kinetoplastid dUTPases have completely different structures compared with the trimeric β-sheet type dUTPase possessed by most organisms, including humans, make the dimeric enzymes attractive drug targets. Here, we present crystal structures of the Leishmania major dUTPase in complex with substrate analogues, the product dUMP and a substrate fragment, and of the homologous Campylobacter jejuni dUTPase in complex with a triphosphate substrate analogue. The metal-binding properties of both enzymes are shown to be dependent upon the ligand identity, a previously unseen characteristic of this family. Furthermore, structures of the Leishmania enzyme in the presence of dUMP and deoxyuridine coupled with tryptophan fluorescence quenching indicate that occupation of the phosphate binding region is essential for induction of the closed conformation and hence for substrate binding. These findings will aid in the development of dUTPase inhibitors as potential new lead anti-trypanosomal compounds.

  15. Fast determination of adenosine 5'-triphosphate (ATP) and its catabolites in royal jelly using ultraperformance liquid chromatography. (United States)

    Zhou, Ling; Xue, XiaoFeng; Zhou, JinHui; Li, Yi; Zhao, Jing; Wu, LiMing


    To obtain insight into the metabolic regulation of adenosine 5'-triphosphate (ATP) in royal jelly and to determine whether ATP and its catabolites can be used as objective parameters to evaluate the freshness and quality of royal jelly (RJ), a rapid ultraperformance liquid chromatography (UPLC) method has been developed for feasible separation and quantitation of ATP and its catabolites in RJ, namely, adenosine 5'-diphosphate (ADP), adenosine 5'-monophosphate (AMP), inosine monophosphate (IMP), inosine (HxR), and hypoxanthine (Hx). The analytes in the sample were extracted using 5% precooled perchloric acid. Chromatographic separation was performed on a Waters Acquity UPLC system with a Waters BEH Shield RP18 column and gradient elution based on a mixture of two solvents: solvent A, 50 mM phosphate buffer (pH 6.5); and solvent B, acetonitrile. The recoveries were in the range of 86.0-102.3% with RSD of no more than 3.6%. The correlation coefficients of six analytes were high (r(2) ≥ 0.9988) and within the test ranges. The limits of detection and quantification for the investigated compounds were lower, at 0.36-0.68 and 1.22-2.30 mg/kg, respectively. The overall intra- and interday RSDs were no more than 1.8%. The developed method was successfully applied to the analysis of the analytes in samples. The results showed that ATP in RJ sequentially degrades to ADP, AMP, IMP, HxR, and Hx during storage.

  16. Calcium and adenosine triphosphate control of cellular pathology: asparaginase-induced pancreatitis elicited via protease-activated receptor 2 (United States)

    Peng, Shuang; Gerasimenko, Julia V.; Tsugorka, Tatiana; Gryshchenko, Oleksiy; Samarasinghe, Sujith; Gerasimenko, Oleg V.


    Exocytotic secretion of digestive enzymes from pancreatic acinar cells is elicited by physiological cytosolic Ca2+ signals, occurring as repetitive short-lasting spikes largely confined to the secretory granule region, that stimulate mitochondrial adenosine triphosphate (ATP) production. By contrast, sustained global cytosolic Ca2+ elevations decrease ATP levels and cause necrosis, leading to the disease acute pancreatitis (AP). Toxic Ca2+ signals can be evoked by products of alcohol and fatty acids as well as bile acids. Here, we have investigated the mechanism by which l-asparaginase evokes AP. Asparaginase is an essential element in the successful treatment of acute lymphoblastic leukaemia, the most common type of cancer affecting children, but AP is a side-effect occurring in about 5–10% of cases. Like other pancreatitis-inducing agents, asparaginase evoked intracellular Ca2+ release followed by Ca2+ entry and also substantially reduced Ca2+ extrusion because of decreased intracellular ATP levels. The toxic Ca2+ signals caused extensive necrosis. The asparaginase-induced pathology depended on protease-activated receptor 2 and its inhibition prevented the toxic Ca2+ signals and necrosis. We tested the effects of inhibiting the Ca2+ release-activated Ca2+ entry by the Ca2+ channel inhibitor GSK-7975A. This markedly reduced asparaginase-induced Ca2+ entry and also protected effectively against the development of necrosis. This article is part of the themed issue ‘Evolution brings Ca2+ and ATP together to control life and death’. PMID:27377732

  17. Supplementation of exogenous adenosine 5'-triphosphate enhances mechanical properties of 3D cell-agarose constructs for cartilage tissue engineering. (United States)

    Gadjanski, Ivana; Yodmuang, Supansa; Spiller, Kara; Bhumiratana, Sarindr; Vunjak-Novakovic, Gordana


    Formation of tissue-engineered cartilage is greatly enhanced by mechanical stimulation. However, direct mechanical stimulation is not always a suitable method, and the utilization of mechanisms underlying mechanotransduction might allow for a highly effective and less aggressive alternate means of stimulation. In particular, the purinergic, adenosine 5'-triphosphate (ATP)-mediated signaling pathway is strongly implicated in mechanotransduction within the articular cartilage. We investigated the effects of transient and continuous exogenous ATP supplementation on mechanical properties of cartilaginous constructs engineered using bovine chondrocytes and human mesenchymal stem cells (hMSCs) encapsulated in an agarose hydrogel. For both cell types, we have observed significant increases in equilibrium and dynamic compressive moduli after transient ATP treatment applied in the fourth week of cultivation. Continuous ATP treatment over 4 weeks of culture only slightly improved the mechanical properties of the constructs, without major changes in the total glycosaminoglycan (GAG) and collagen content. Structure-function analyses showed that transiently ATP-treated constructs, and in particular those based on hMSCs, had the highest level of correlation between compositional and mechanical properties. Transiently treated groups showed intense staining of the territorial matrix for GAGs and collagen type II. These results indicate that transient ATP treatment can improve functional mechanical properties of cartilaginous constructs based on chondrogenic cells and agarose hydrogels, possibly by improving the structural organization of the bulk phase and territorial extracellular matrix (ECM), that is, by increasing correlation slopes between the content of the ECM components (GAG, collagen) and mechanical properties of the construct.

  18. Synthesis of deoxynucleoside triphosphates that include proline, urea, or sulfonamide groups and their polymerase incorporation into DNA. (United States)

    Hollenstein, Marcel


    To expand the chemical array available for DNA sequences in the context of in vitro selection, I present herein the synthesis of five nucleoside triphosphate analogues containing side chains capable of organocatalysis. The synthesis involved the coupling of L-proline-containing residues (dU(tP)TP and dU(cP)TP), a dipeptide (dU(FP)TP), a urea derivative (dU(Bpu)TP), and a sulfamide residue (dU(Bs)TP) to a suitably protected common intermediate, followed by triphosphorylation. These modified dNTPs were shown to be excellent substrates for the Vent (exo(-)) and Pwo DNA polymerases, as well as the Klenow fragment of E. coli DNA polymerase I, although they were only acceptable substrates for the 9°N(m) polymerase. All of the modified dNTPs, with the exception of dU(Bpu)TP, were readily incorporated into DNA by the polymerase chain reaction (PCR). Modified oligonucleotides efficiently served as templates for PCR for the regeneration of unmodified DNA. Thermal denaturation experiments showed that these modifications are tolerated in the major groove. Overall, these heavily modified dNTPs are excellent candidates for SELEX.

  19. Action of angiotensin II, 5-hydroxytryptamine and adenosine triphosphate on ionic currents in single ear artery cells of the rabbit. (United States)

    Hughes, A D; Bolton, T B


    1. Angiotensin II, 5-hydroxytryptamine (5-HT) and adenosine triphosphate (ATP) evoked a transient inward current in isolated single car artery cells of rabbit held at -60 mV by whole cell voltage clamp in physiological saline using a KCL-containing pipette solution. Under these conditions agonist did not activate a calcium-dependent potassium current. 2. Responses to each agonist were transient and desensitized rapidly. Inward current at -60 mV holding potential was not abolished by blockade of voltage-dependent calcium channels or by buffering intracellular calcium with BAPTA, a calcium chelator, or following depletion of intracellular calcium stores with ryanodine. 3. The shape of the current-voltage relationships and the reversal potentials of the current induced by angiotensin II, 5-HT and ATP were similar under a variety of ionic conditions. Agonist-induced current was unaffected by replacing intracellular chloride with citrate ions or by replacing intracellular sodium with caesium or extracellular sodium with barium or calcium. Replacement of extracellular sodium with Tris shifted the reversal potential in all cases by around 30 mV negatively. 4. These data suggest that angiotensin II, 5-HT and ATP activate similar cationic conductances which are relatively non-selective allowing mono- and divalent cations to cross the smooth muscle cell membrane. These channels may allow the influx of calcium under physiological conditions.

  20. The role of microorganisms in the degradation of adenosine triphosphate (ATP) in chill-stored common carp (Cyprinus carpio) fillets. (United States)

    Li, Dapeng; Zhang, Longteng; Song, Sijia; Wang, Zhiying; Kong, Chunli; Luo, Yongkang


    Biochemical and microbial changes after harvest strongly affect the final quality and shelf life of fish and fish products. In this study, the role of microbes in the degradation of adenosine triphosphate (ATP), and the origin of adenosine monophosphate deaminase (AMPD) and acid phosphatase (ACP) in common carp fillets during different stages of chilled storage (at 4°C) were investigated. The content of ATP, ADP, AMP, IMP, HxR, and Hx, the activity of AMPD and ACP, and the total count of viable, Aeromonas, Pseudomonas, H2S-producing bacteria, and lactic acid bacteria were examined. Results indicated that the population of microbial communities in control samples increased with storage time, and Pseudomonas peaked on the 10th day of storage. Changes in AMPD activity were less related to the abundance of microbes during the entire storage period. However, ACP was derived from both fish muscle and microbial secretion during the middle and late stages of storage. Degradation of ATP to IMP was not affected by spoilage bacteria, but the hydrolysis of IMP, and the transformation of HxR to Hx was affected considerably by the spoilage bacteria.

  1. Control of tunneling by adapted signals

    CERN Document Server

    Ivlev, B I


    Process of quantum tunneling of particles in various physical systems can be effectively controlled even by a weak and slow varying in time electromagnetic signal if to adapt specially its shape to a particular system. During an under-barrier motion of a particle such signal provides a "coherent" assistance of tunneling by the multi-quanta absorption resulting in a strong enhancement of the tunneling probability. The semiclassical approach based on trajectories in the complex time is developed for tunneling in a non-stationary field. Enhancement of tunneling occurs when a singularity of the signal coincides in position at the complex time plane with a singularity of the classical Newtonian trajectory of the particle. The developed theory is also applicable to the over-barrier reflection of particles and to reflection of classical waves (electromagnetic, hydrodynamic, etc.) from a spatially-smooth medium.

  2. Detecting DNS Tunnels Using Character Frequency Analysis

    CERN Document Server

    Born, Kenton


    High-bandwidth covert channels pose significant risks to sensitive and proprietary information inside company networks. Domain Name System (DNS) tunnels provide a means to covertly infiltrate and exfiltrate large amounts of information passed network boundaries. This paper explores the possibility of detecting DNS tunnels by analyzing the unigram, bigram, and trigram character frequencies of domains in DNS queries and responses. It is empirically shown how domains follow Zipf's law in a similar pattern to natural languages, whereas tunneled traffic has more evenly distributed character frequencies. This approach allows tunnels to be detected across multiple domains, whereas previous methods typically concentrate on monitoring point to point systems. Anomalies are quickly discovered when tunneled traffic is compared to the character frequency fingerprint of legitimate domain traffic.

  3. Concept development for HLW disposal research tunnel

    Energy Technology Data Exchange (ETDEWEB)

    Queon, S. K.; Kim, K. S.; Park, J. H.; Jeo, W. J.; Han, P. S. [KAERI, Taejon (Korea, Republic of)


    In order to dispose high-level radioactive waste in a geological formation, it is necessary to assess the safety of a disposal concept by excavating a research tunnel in the same geological formation as the host rock mass. The design concept of a research tunnel depends on the actual disposal concept, repository geometry, experiments to be carried at the tunnel, and geological conditions. In this study, analysis of the characteristics of the disposal research tunnel, which is planned to be constructed at KAERI site, calculation of the influence of basting impact on neighbor facilities, and computer simuation for mechanical stability analysis using a three-dimensional code, FLAC3D, had been carried out to develop the design concept of the research tunnel.

  4. Characterization of magnetic tunnel junction test pads

    DEFF Research Database (Denmark)

    Østerberg, Frederik Westergaard; Kjær, Daniel; Nielsen, Peter Folmer


    We show experimentally as well as theoretically that patterned magnetic tunnel junctions can be characterized using the current-in-plane tunneling (CIPT) method, and the key parameters, the resistance-area product (RA) and the tunnel magnetoresistance (TMR), can be determined. The CIPT method...... on square tunnel junction pads with varying sizes and analyze the measured data using both the original and the modified CIPT model. Thus, we determine in which sample size range the modified CIPT model is needed to ensure validity of the extracted sample parameters, RA and TMR. In addition, measurements...... as a function of position on a square tunnel junction pad are used to investigate the sensitivity of the measurement results to probe misalignment....

  5. IPv6 Tunneling Over IPV4

    Directory of Open Access Journals (Sweden)

    A.Sankara Narayanan


    Full Text Available Due to the huge growth of the internet users, mobile users using internet connection makes development and implementation of IPv6 as an alternate solution. IPv6 is a long anticipated upgrade to the internets main communication protocol, which is called IPv4. The current address space provided by IPv4, with only4, 294, 967, 296 addresses. Nowadays IPv6 tunneling over IPv4 are widely used to form the global IPv6 Internet. The IPv6 128-bit address scheme it should provide enough addresses for everyones computer. Tunneling provides a path to use an existing IPv4 to IPv6. This paper describes typical IPv6 tunneling and tunnel brokers deployment in real IP networks. In the deployment of IPv6, it is a well adopted practice that IPv6 networks are interconnected via IPv6 over IPv4 (IPv6/IPv4 tunnels.

  6. Experimental evidence for Wigner's tunneling time

    CERN Document Server

    Camus, Nicolas; Fechner, Lutz; Klaiber, Michael; Laux, Martin; Mi, Yonghao; Hatsagortsyan, Karen Z; Pfeifer, Thomas; Keitel, Christoph H; Moshammer, Robert


    Tunneling of a particle through a potential barrier remains one of the most remarkable quantum phenomena. Owing to advances in laser technology, electric fields comparable to those electrons experience in atoms are readily generated and open opportunities to dynamically investigate the process of electron tunneling through the potential barrier formed by the superposition of both laser and atomic fields. Attosecond-time and angstrom-space resolution of the strong laser-field technique allow to address fundamental questions related to tunneling, which are still open and debated: Which time is spent under the barrier and what momentum is picked up by the particle in the meantime? In this combined experimental and theoretical study we demonstrate that for strong-field ionization the leading quantum mechanical Wigner treatment for the time resolved description of tunneling is valid. We achieve a high sensitivity on the tunneling barrier and unambiguously isolate its effects by performing a differential study of t...

  7. Snow and ice blocking of tunnels

    Energy Technology Data Exchange (ETDEWEB)

    Lia, Leif


    Hydroelectric power development in cold regions causes much concern about operational reliability and dam safety. This thesis studies the temperature distribution in tunnels by means of air temperature measurements in six tunnel spillways and five diversion tunnels. The measurements lasted for two consecutive winters. The air through flow tunnel is used as it causes cooling of both rock and water. In open spillway tunnels, frost reaches the entire tunnel. In spillway tunnels with walls, the frost zones reach about 100 m from the downstream end. In mildly-inclined diversion tunnels, a frost free zone is located in the middle of the tunnel and snow and ice problems were only observed in the inlet and outlet. Severe aufeis is accumulation is observed in the frost zones. The heat transfer from rock to air, water and ice is calculated and used in a prediction model for the calculation of aufeis build-up together with local field observation data. The water penetration of snow plugs is also calculated, based on the heat balance. It takes 20 to 50 days for water to enter the blocked tunnel. The empirical values are 30 to 60 days, but only 1 day if the temperature of the snow pack is 0{sup o}C. Sensitivity analyses are carried out for temperature variations in rock, snow, water and ice. Systematic field observation shows that it is important for hydropower companies to know about the effects of snow and ice blocking in an area. A risk analysis of dam safety is presented for a real case. Finally, the thesis proposes solutions which can reduce the snow and ice problems. 79 refs., 63 figs., 11 tabs.

  8. In Vivo Quantification of Active Decitabine-Triphosphate Metabolite: A Novel Pharmacoanalytical Endpoint for Optimization of Hypomethylating Therapy in Acute Myeloid Leukemia


    Wang, Hongyan; Chen, Ping; Wang, Jiang; Santhanam, Ramasamy; Aimiuwu, Josephine; Saradhi, U. V. Vijaya; Liu, Zhongfa; Schwind, Sebastian; Mims, Alice; Byrd, John C.; Grever, Michael R.; Villalona-Calero, Miguel A.; Klisovic, Rebecca; Walker, Alison; Garzon, Ramiro


    Decitabine (DAC) is used for treatment of patients with myelodysplastic syndromes and acute myeloid leukemia (AML). Following cellular uptake, DAC is activated to DAC-triphosphate (TP) and incorporated into DNA. Once incorporated into the DNA, DAC-TP binds and inactivates DNA methyltransferases (DNMTs), thereby leading to hypomethylation and re-expression of epigenetically silenced tumor suppressor genes and ultimately antileukemia activity. However, direct evidence of in vivo DAC-TP occurren...

  9. Effects of oral adenosine-5′-triphosphate supplementation on athletic performance, skeletal muscle hypertrophy and recovery in resistance-trained men


    Wilson, Jacob M; Joy, Jordan M; Ryan P. Lowery; Roberts, Michael D.; Lockwood, Christopher M; Manninen, Anssi H; Fuller, John C; Souza, Eduardo O.; Baier, Shawn M.; Wilson, Stephanie MC; Rathmacher, John A


    Background Currently, there is a lack of studies examining the effects of adenosine-5′-triphosphate (ATP) supplementation utilizing a long-term, periodized resistance-training program (RT) in resistance-trained populations. Therefore, we investigated the effects of 12 weeks of 400 mg per day of oral ATP on muscular adaptations in trained individuals. We also sought to determine the effects of ATP on muscle protein breakdown, cortisol, and performance during an overreaching cycle. Methods The ...

  10. Interfering with bacterial gossip

    DEFF Research Database (Denmark)

    Bjarnsholt, Thomas; Tolker-Nielsen, Tim; Givskov, Michael


    defense. Antibiotics exhibit a rather limited effect on biofilms. Furthermore, antibiotics have an ‘inherent obsolescence’ because they select for development of resistance. Bacterial infections with origin in bacterial biofilms have become a serious threat in developed countries. Pseudomonas aeruginosa...... that appropriately target bacteria in their relevant habitat with the aim of mitigating their destructive impact on patients. In this review we describe molecular mechanisms involved in “bacterial gossip” (more scientifically referred to as quorum sensing (QS) and c-di-GMP signaling), virulence, biofilm formation......, resistance and QS inhibition as future antimicrobial targets, in particular those that would work to minimize selection pressures for the development of resistant bacteria....

  11. Effects of diesel exhaust on the microbiota within a tuffaceous tunnel system

    Energy Technology Data Exchange (ETDEWEB)

    Haldeman, D.L.; Lagadinos, T.; Amy, P.S. [Univ. of Nevada, Las Vegas, NV (United States); Hersman, L. [Los Alamos National Lab., NM (United States); Meike, A. [Lawrence Livermore National Lab., Livermore, CA (United States)


    The abundance and distribution of microbiota that may be impacted by diesel and diesel exhaust were investigated from three depths into the walls and invert (floor) of U12n tunnel at Rainier Mesa, Nevada Test Site, a potential geological analog of Yucca Mountain. Enumerations included total cell counts, and numbers of aerobic heterotrophic, sulfate-reducing, nitrate-reducing, and diesel-degrading bacteria. Additionally, the disappearance of total petroleum hydrocarbons was determined in microcosms containing subsurface materials that were amended with diesel fuel. Results revealed that microbes capable of utilizing diesel and diesel combustion products were present in the subsurface in both the walls and the invert of the tunnel. The abundance of specific bacterial types in the tunnel invert, a perturbed environment, was greater than that observed in the tunnel wall. Few trends of microbial distribution either into the tunnel wall or the invert were noted with the exception of aerobic heterotrophic abundance which increased with depth into the wall and decreased with depth into the invert. No correlation between microbiota and a specific introduced chemical species have yet been determined. The potential for microbial contamination of the tunnel wall during sampling was determined to be negligible by the use of fluorescently labeled latex spheres (1{mu}m in dia.) as tracers. Results indicate that additional investigations might be needed to examine the microbiota and their possible impacts on the geology and geochemistry of the subsurface, both indigenous microbiota and those microorganisms that will likely be introduced by anthropogenic activity associated with the construction of a high-level waste repository.

  12. Quantitative risk assessment modeling for nonhomogeneous urban road tunnels. (United States)

    Meng, Qiang; Qu, Xiaobo; Wang, Xinchang; Yuanita, Vivi; Wong, Siew Chee


    Urban road tunnels provide an increasingly cost-effective engineering solution, especially in compact cities like Singapore. For some urban road tunnels, tunnel characteristics such as tunnel configurations, geometries, provisions of tunnel electrical and mechanical systems, traffic volumes, etc. may vary from one section to another. These urban road tunnels that have characterized nonuniform parameters are referred to as nonhomogeneous urban road tunnels. In this study, a novel quantitative risk assessment (QRA) model is proposed for nonhomogeneous urban road tunnels because the existing QRA models for road tunnels are inapplicable to assess the risks in these road tunnels. This model uses a tunnel segmentation principle whereby a nonhomogeneous urban road tunnel is divided into various homogenous sections. Individual risk for road tunnel sections as well as the integrated risk indices for the entire road tunnel is defined. The article then proceeds to develop a new QRA model for each of the homogeneous sections. Compared to the existing QRA models for road tunnels, this section-based model incorporates one additional top event-toxic gases due to traffic congestion-and employs the Poisson regression method to estimate the vehicle accident frequencies of tunnel sections. This article further illustrates an aggregated QRA model for nonhomogeneous urban tunnels by integrating the section-based QRA models. Finally, a case study in Singapore is carried out.

  13. Area and shape changes of the carpal tunnel in response to tunnel pressure. (United States)

    Li, Zong-Ming; Masters, Tamara L; Mondello, Tracy A


    Carpal tunnel mechanics is relevant to our understanding of median nerve compression in the tunnel. The compliant characteristics of the tunnel strongly influence its mechanical environment. We investigated the distensibility of the carpal tunnel in response to tunnel pressure. A custom balloon device was designed to apply controlled pressure. Tunnel cross sections were obtained using magnetic resonance imaging to derive the relationship between carpal tunnel pressure and morphological parameters at the hook of hamate. The results showed that the cross-sectional area (CSA) at the level of the hook of hamate increased, on average, by 9.2% and 14.8% at 100 and 200 mmHg, respectively. The increased CSA was attained by a shape change of the cross section, displaying increased circularity. The increase in CSA was mainly attributable to the increase of area in the carpal arch region formed by the transverse carpal ligament. The narrowing of the carpal arch width was associated with an increase in the carpal arch. We concluded that the carpal tunnel is compliant to accommodate physiological variations of the carpal tunnel pressure, and that the increase in tunnel CSA is achieved by increasing the circularity of the cross section.

  14. Tunneling magnetoresistance of silicon chains (United States)

    Matsuura, Yukihito


    The tunneling magnetoresistance (TMR) of a silicon chain sandwiched between nickel electrodes was examined by using first-principles density functional theory. The relative orientation of the magnetization in a parallel-alignment (PA) configuration of two nickel electrodes enhanced the current with a bias less than 0.4 V compared with that in an antiparallel-alignment configuration. Consequently, the silicon chain-nickel electrodes yielded good TMR characteristics. In addition, there was polarized spin current in the PA configuration. The spin polarization of sulfur atoms functioning as a linking bridge between the chain and nickel electrode played an important role in the magnetic effects of the electric current. Moreover, the hybridization of the sulfur 3p orbital and σ-conjugated silicon 3p orbital contributed to increasing the total current.

  15. Tunnel effect wave energy detection (United States)

    Kaiser, William J. (Inventor); Waltman, Steven B. (Inventor); Kenny, Thomas W. (Inventor)


    Methods and apparatus for measuring gravitational and inertial forces, magnetic fields, or wave or radiant energy acting on an object or fluid in space provide an electric tunneling current through a gap between an electrode and that object or fluid in space and vary that gap with any selected one of such forces, magnetic fields, or wave or radiant energy acting on that object or fluid. These methods and apparatus sense a corresponding variation in an electric property of that gap and determine the latter force, magnetic fields, or wave or radiant energy in response to that corresponding variation, and thereby sense or measure such parameters as acceleration, position, particle mass, velocity, magnetic field strength, presence or direction, or wave or radiant energy intensity, presence or direction.

  16. Hawking Radiation and Classical Tunneling

    CERN Document Server

    Tracy, Eugene R


    Acoustic waves in fluids undergoing the transition from sub- to supersonic flow satisfy governing equations similar to those for light waves in the immediate vicinity of a black hole event horizon. This acoustic analogy has been used by Unruh and others as a conceptual model for `Hawking radiation.' Here we use variational methods, originally introduced by Brizard for the study of linearized MHD, and ray phase space methods, to analyze linearized acoustics in the presence of background flows. The variational formulation endows the evolution equations with natural Hermitian and symplectic structures that prove useful for later analysis. We derive a $2\\times 2$ normal form governing the wave evolution in the vicinity of the `event horizon.' This shows that the acoustic model can be reduced locally (in ray phase space) to a standard (scalar) tunneling process weakly coupled to a unidirectional non-dispersive wave (the `incoming wave'). Given the normal form, the Hawking `thermal spectrum' can be derived by invok...

  17. Direct Approach to Quantum Tunneling (United States)

    Andreassen, Anders; Farhi, David; Frost, William; Schwartz, Matthew D.


    The decay rates of quasistable states in quantum field theories are usually calculated using instanton methods. Standard derivations of these methods rely in a crucial way upon deformations and analytic continuations of the physical potential and on the saddle-point approximation. While the resulting procedure can be checked against other semiclassical approaches in some one-dimensional cases, it is challenging to trace the role of the relevant physical scales, and any intuitive handle on the precision of the approximations involved is at best obscure. In this Letter, we use a physical definition of the tunneling probability to derive a formula for the decay rate in both quantum mechanics and quantum field theory directly from the Minkowski path integral, without reference to unphysical deformations of the potential. There are numerous benefits to this approach, from nonperturbative applications to precision calculations and aesthetic simplicity.

  18. Theory of dissociative tunneling ionization

    CERN Document Server

    Svensmark, Jens; Madsen, Lars Bojer


    We present a theoretical study of the dissociative tunneling ionization process. Analytic expressions for the nuclear kinetic energy distribution of the ionization rates are derived. A particularly simple expression for the spectrum is found by using the Born-Oppenheimer (BO) approximation in conjunction with the reflection principle. These spectra are compared to exact non-BO ab initio spectra obtained through model calculations with a quantum mechanical treatment of both the electronic and nuclear degrees freedom. In the regime where the BO approximation is applicable imaging of the BO nuclear wave function is demonstrated to be possible through reverse use of the reflection principle, when accounting appropriately for the electronic ionization rate. A qualitative difference between the exact and BO wave functions in the asymptotic region of large electronic distances is shown. Additionally the behavior of the wave function across the turning line is seen to be reminiscent of light refraction. For weak fiel...

  19. Bacterial intermediate filaments

    DEFF Research Database (Denmark)

    Charbon, Godefroid; Cabeen, M.; Jacobs-Wagner, C.


    Crescentin, which is the founding member of a rapidly growing family of bacterial cytoskeletal proteins, was previously proposed to resemble eukaryotic intermediate filament (IF) proteins based on structural prediction and in vitro polymerization properties. Here, we demonstrate that crescentin...

  20. Bacterial Wound Culture (United States)

    ... Home Visit Global Sites Search Help? Bacterial Wound Culture Share this page: Was this page helpful? Also known as: Aerobic Wound Culture; Anaerobic Wound Culture Formal name: Culture, wound Related ...

  1. Bacterial surface adaptation (United States)

    Utada, Andrew


    Biofilms are structured multi-cellular communities that are fundamental to the biology and ecology of bacteria. Parasitic bacterial biofilms can cause lethal infections and biofouling, but commensal bacterial biofilms, such as those found in the gut, can break down otherwise indigestible plant polysaccharides and allow us to enjoy vegetables. The first step in biofilm formation, adaptation to life on a surface, requires a working knowledge of low Reynolds number fluid physics, and the coordination of biochemical signaling, polysaccharide production, and molecular motility motors. These crucial early stages of biofilm formation are at present poorly understood. By adapting methods from soft matter physics, we dissect bacterial social behavior at the single cell level for several prototypical bacterial species, including Pseudomonas aeruginosa and Vibrio cholerae.

  2. Bacterial Meningitis in Infants

    Directory of Open Access Journals (Sweden)

    J Gordon Millichap


    Full Text Available A retrospective study of 80 infantile patients (ages 30-365 days; 47 male, 33 female with culture-proven bacterial meningitis seen over a 16 year period (1986-2001 is reported from Taiwan.

  3. Rudolf Hermann, wind tunnels and aerodynamics (United States)

    Lundquist, Charles A.; Coleman, Anne M.


    Rudolf Hermann was born on December 15, 1904 in Leipzig, Germany. He studied at the University of Leipzig and at the Aachen Institute of Technology. His involvement with wind tunnels began in 1934 when Professor Carl Wieselsberger engaged him to work at Aachen on the development of a supersonic wind tunnel. On January 6, 1936, Dr. Wernher von Braun visited Dr. Hermann to arrange for use of the Aachen supersonic wind tunnel for Army problems. On April 1, 1937, Dr. Hermann became Director of the Supersonic Wind Tunnel at the Army installation at Peenemunde. Results from the Aachen and Peenemunde wind tunnels were crucial in achieving aerodynamic stability for the A-4 rocket, later designated as the V-2. Plans to build a Mach 10 'hypersonic' wind tunnel facility at Kochel were accelerated after the Allied air raid on Peenemunde on August 17, 1943. Dr. Hermann was director of the new facility. Ignoring destruction orders from Hitler as WWII approached an end in Europe, Dr. Hermann and his associates hid documents and preserved wind tunnel components that were acquired by the advancing American forces. Dr. Hermann became a consultant to the Air Force at its Wright Field in November 1945. In 1951, he was named professor of Aeronautical Engineering at the University of Minnesota. In 1962, Dr. Hermann became the first Director of the Research Institute at the University of Alabama in Huntsville (UAH), a position he held until he retired in 1970.

  4. Pair Tunneling through Single Molecules (United States)

    Raikh, Mikhail


    Coupling to molecular vibrations induces a polaronic shift, and can lead to a negative charging energy, U. For negative U, the occupation of the ground state of the molecule is even. In this situation, virtual pair transitions between the molecule and the leads can dominate electron transport. At low temperature, T, these transitions give rise to the charge-Kondo effect [1]. We developed the electron transport theory through the negative-U molecule [2] at relatively high T, when the Kondo correlations are suppressed. Two physical ingredients distinguish our theory from the transport through a superconducting grain coupled to the normal leads [3]: (i) in parallel with sequential pair-tunneling processes, single-particle cotunneling processes take place; (ii) the electron pair on the molecule can be created (or annihilated) by two electrons tunneling in from (or out to) opposite leads. We found that, even within the rate-equation description, the behavior of differential conductance through the negative-U molecule as function of the gate voltage is quite peculiar: the height of the peak near the degeneracy point is independent of temperature, while its width is proportional to T. This is in contrast to the ordinary Coulomb-blockade conductance peak, whose integral strength is T-independent. At finite source-drain bias, V>>T, the width of the conductance peak is ˜V, whereas the conventional Coulomb-blockade peak at finite V splits into two sharp peaks at detunings V/2, and -V/2. Possible applications to the gate-controlled current rectification and switching will be discussed. [1] A. Taraphder and P. Coleman, Phys. Rev. Lett. 66, 2814 (1991). [2] J. Koch, M. E. Raikh, and F. von Oppen, Phys. Rev. Lett. 96, 056803 (2006). [3] F. W. J. Hekking, L. I. Glazman, K. A. Matveev, and R. I. Shekhter, Phys. Rev. Lett. 70, 4138 (1993).

  5. Bacterial proteases and virulence

    DEFF Research Database (Denmark)

    Frees, Dorte; Brøndsted, Lone; Ingmer, Hanne


    Bacterial pathogens rely on proteolysis for variety of purposes during the infection process. In the cytosol, the main proteolytic players are the conserved Clp and Lon proteases that directly contribute to virulence through the timely degradation of virulence regulators and indirectly by providing....... These extracellular proteases are activated in complex cascades involving auto-processing and proteolytic maturation. Thus, proteolysis has been adopted by bacterial pathogens at multiple levels to ensure the success of the pathogen in contact with the human host....

  6. [Diagnosis of bacterial vaginosis]. (United States)

    Djukić, Slobodanka; Ćirković, Ivana; Arsić, Biljana; Garalejić, Eliana


    Bacterial vaginosis is a common, complex clinical syndrome characterized by alterations in the normal vaginal flora. When symptomatic, it is associated with a malodorous vaginal discharge and on occasion vaginal burning or itching. Under normal conditions, lactobacilli constitute 95% of the bacteria in the vagina. Bacterial vaginosis is associated with severe reduction or absence of the normal H2O2-producing lactobacilli and overgrowth of anaerobic bacteria and Gardnerella vaginalis, Atopobium vaginae, Mycoplasma hominis and Mobiluncus species. Most types of infectious disease are diagnosed by culture, by isolating an antigen or RNA/DNA from the microbe, or by serodiagnosis to determine the presence of antibodies to the microbe. Therefore, demonstration of the presence of an infectious agent is often a necessary criterion for the diagnosis of the disease. This is not the case for bacterial vaginosis, since the ultimate cause of the disease is not yet known. There are a variety of methods for the diagnosis of bacterial vaginosis but no method can at present be regarded as the best. Diagnosing bacterial vaginosis has long been based on the clinical criteria of Amsel, whereby three of four defined criteria must be satisfied. Nugent's scoring system has been further developed and includes validation of the categories of observable bacteria structures. Up-to-date molecular tests are introduced, and better understanding of vaginal microbiome, a clear definition for bacterial vaginosis, and short-term and long-term fluctuations in vaginal microflora will help to better define molecular tests within the broader clinical context.

  7. Application of Important Factors in Tunnel Projects

    Directory of Open Access Journals (Sweden)

    Siamak Bagherian


    Full Text Available The objective of this thesis is to improve the quality of the basis for making decisions about tender prices and budgets for tunnel projects by developing a model for the estimation of construction time and cost. The planning and constructing of extensions to existing road and railway networks is an ongoing mission of transport infrastructure development. For functional, aesthetic or environmental reasons, a large number of these extensions are planned as tunnels. In the planning and procurement phases of tunnel projects, numerous decisions have to be made in relation to the tender price and project budget.

  8. Carpal tunnel syndrome - anatomical and clinical correlations. (United States)

    Iskra, Tomasz; Mizia, Ewa; Musial, Agata; Matuszyk, Aleksandra; Tomaszewski, Krzysztof A


    Carpal tunnel syndrome (CTS) is the most common and widely known of the entrapment neuropathies in which the body's peripheral nerves are compressed. Common symptoms of CTS involve the hand and result from compression of the median nerve within the carpal tunnel. In general, CTS develops when the tissues around the median nerve irritate or compress on the nerve along its course through the carpal tunnel, however often it is very difficult to determine cause of CTS. Proper treatment (conservative or surgical) usually can relieve the symptoms and restore normal use of the wrist and hand.


    Institute of Scientific and Technical Information of China (English)

    RUAN Shi-ping; WU Jian-hua; WU Wei-wei; XI Ru-ze


    The selection of the configuration and size of an aerator was of importance for a tunnel spillway under the conditions of high speed flows. Experimental investigations were conducted on the effects of entrained air on the tunnel spillway in the Goupitan Project, based on the criterion of gravity similarity and the condition of aerated flow velocity of over 6 m/s, with physical models. The configurations of the aerators were presented of a larger bottom air concentration, to protect the tunnel spillway from cavitation as well as to see no water fills in the grooves.

  10. The Thames Tideway Tunnel (3/3)

    CERN Document Server

    CERN. Geneva


    Lecture 3: Insight into a pioneering project at the cutting edge of engineering: the upgrade to London’s failing sewerage system. With a growing population and heavier rainfall, the River Thames is regularly polluted in breach of European Directive requirements. Two new storage and transfer tunnels will run up to 85m deep under the river and will intercept and divert sewer overflows to a treatment facility in east London. The challenges faced by constructing a tunnel project of this size under the river and through London’s historic urban environment will set a new UK record for this type of tunnelling.

  11. Extraskeletal chondroma casuing carpal tunnel syndrome: A case report

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Ok Hwa; Kim, Yeon Hee [Haeundae Paik Hospital, Inje University College of Medicine, Busan (Korea, Republic of)


    Carpal tunnel syndrome caused by extraskeletal chondroma has been scarcely reported in the literature. Authors report a case of carpal tunnel syndrome as a result of an extraskeletal chondroma arising within the carpal tunnel, and describe the radiological and pathological findings of the mass. We also discuss the differential diagnosis of the calcified space, occupying lesions that may occur in carpal tunnel.

  12. Pressure-morphology relationship of a released carpal tunnel. (United States)

    Kim, Dong Hee; Marquardt, Tamara L; Gabra, Joseph N; Shen, Zhilei Liu; Evans, Peter J; Seitz, William H; Li, Zong-Ming


    We investigated morphological changes of a released carpal tunnel in response to variations of carpal tunnel pressure. Pressure within the carpal tunnel is known to be elevated in patients with carpal tunnel syndrome and dependent on wrist posture. Previously, increased carpal tunnel pressure was shown to affect the morphology of the carpal tunnel with an intact transverse carpal ligament (TCL). However, the pressure-morphology relationship of the carpal tunnel after release of the TCL has not been investigated. Carpal tunnel release (CTR) was performed endoscopically on cadaveric hands and the carpal tunnel pressure was dynamically increased from 10 to 120 mmHg. Simultaneously, carpal tunnel cross-sectional images were captured by an ultrasound system, and pressure measurements were recorded by a pressure transducer. Carpal tunnel pressure significantly affected carpal arch area (p 62 mm(2) at 120 mmHg. Carpal arch height, length, and width also significantly changed with carpal tunnel pressure (p carpal tunnel pressure increased, carpal arch height and length increased, but the carpal arch width decreased. Analyses of the pressure-morphology relationship for a released carpal tunnel revealed a nine times greater compliance than that previously reported for a carpal tunnel with an intact TCL. This change of structural properties as a result of transecting the TCL helps explain the reduction of carpal tunnel pressure and relief of symptoms for patients after CTR surgery.

  13. Diastolic Dysfunction Induced by a High-Fat Diet Is Associated with Mitochondrial Abnormality and Adenosine Triphosphate Levels in Rats

    Directory of Open Access Journals (Sweden)

    Ki-Woon Kang


    Full Text Available BackgroundObesity is well-known as a risk factor for heart failure, including diastolic dysfunction. However, this mechanism in high-fat diet (HFD-induced obese rats remain controversial. The purpose of this study was to investigate whether cardiac dysfunction develops when rats are fed with a HFD for 10 weeks; additionally, we sought to investigate the association between mitochondrial abnormalities, adenosine triphosphate (ATP levels and cardiac dysfunction.MethodsWe examined myocardia in Wistar rats after 10 weeks of HFD (45 kcal% fat, n=6 or standard diet (SD, n=6. Echocardiography, histomorphologic analysis, and electron microscopy were performed. The expression levels of mitochondrial oxidative phosphorylation (OXPHOS subunit genes, peroxisome-proliferator-activated receptor γ co-activator-1α (PGC1α and anti-oxidant enzymes were assessed. Markers of oxidative stress damage, mitochondrial DNA copy number and myocardial ATP level were also examined.ResultsAfter 10 weeks, the body weight of the HFD group (349.6±22.7 g was significantly higher than that of the SD group (286.8±14.9 g, and the perigonadal and epicardial fat weights of the HFD group were significantly higher than that of the SD group. Histomorphologic and electron microscopic images were similar between the two groups. However, in the myocardium of the HFD group, the expression levels of OXPHOS subunit NDUFB5 in complex I and PGC1α, and the mitochondrial DNA copy number were decreased and the oxidative stress damage marker 8-hydroxydeoxyguanosine was increased, accompanied by reduced ATP levels.ConclusionDiastolic dysfunction was accompanied by the mitochondrial abnormality and reduced ATP levels in the myocardium of 10 weeks-HFD-induced rats.

  14. Application of adenosine 5'-triphosphate (ATP infusions in palliative home care: design of a randomized clinical trial

    Directory of Open Access Journals (Sweden)

    van den Borne Ben E


    Full Text Available Abstract Background Palliative care in cancer aims at alleviating the suffering of patients. A previous study in patients with advanced non-small-cell lung cancer showed that adenosine 5'-triphosphate (ATP infusions had a favourable effect on fatigue, appetite, body weight, muscle strength, functional status and quality of life. The present study was designed 1. To evaluate whether ATP has favourable effects in terminally ill cancer patients, 2. To evaluate whether ATP infusions may reduce family caregiver burden and reduce the use of professional health care services, and 3. To test the feasibility of application of ATP infusions in a home care setting. Methods/Design The study can be characterized as an open-labelled randomized controlled trial with two parallel groups. The intervention group received usual palliative care, two visits by an experienced dietician for advice, and regular ATP infusions over a period of 8 weeks. The control group received palliative care as usual and dietetic advice, but no ATP. This paper gives a description of the study design, selection of patients, interventions and outcome measures. Discussion From April 2002 through October 2006, a total of 100 patients have been randomized. Follow-up of patients will be completed in December 2006. At the time of writing, five patients are still in follow up. Of the 95 patients who have completed the study, 69 (73% have completed four weeks of follow-up, and 53 (56% have completed the full eight-week study period. The first results are expected in 2007.

  15. Interaction of 2'-deoxyguanosine triphosphate analogue inhibitors of HIV reverse transcriptase with human mitochondrial DNA polymerase gamma. (United States)

    Ray, Adrian S; Feng, Joy Y; Murakami, Eisuke; Chu, Chung K; Schinazi, Raymond F; Anderson, Karen S


    Mitochondrial toxicity is a limiting factor in the use of some nucleoside reverse transcriptase inhibitors of HIV. To further understand the impact of structural features on the incorporation and exonuclease removal of nucleoside monophosphate (MP) analogues by human mitochondrial DNA polymerase (pol gamma), transient kinetic studies were done with analogues of 2'-deoxyguanosine triphosphate. The kinetic parameters for the incorporation and removal of carbovir (CBV)-MP, dioxolane guanosine (DXG)-MP and 2',3'-dideoxy-2',3'-didehydroguanosine (d4G)-MP were studied with pol gamma holoenzyme. The importance of the ribose oxygen in incorporation by pol gamma was illustrated by an approximate 3,000-fold decrease in the incorporation efficiency of an analogue lacking the ribose oxygen (CBV-TP) relative to those containing a ribose oxygen (DXG-TP and d4G-TP). As a result, a comparison with previous data for the incorporation by HIV reverse transcriptase showed CBV-TP to be approximately 800-8,000-fold more selective for its antiviral target over pol gamma relative to the other guanosine analogues. However, DXG-TP and d4G-TP were found to be much more selective than previously reported values for mitochondrial toxic nucleoside analogues. Structural modelling based on sequence homology with other polymerase A family members suggests that an interaction between the ribose oxygen and arginine 853 in pol gamma may play a critical role in causing this differential incorporation. Exonuclease removal of a chain-terminating CBV-MP was also found to be more efficient by pol gamma. These results help to further elucidate the structure activity relationships for pol gamma and should aid in the design of more selective antiviral agents.

  16. Adenosine Triphosphate (ATP Is a Candidate Signaling Molecule in the Mitochondria-to-Nucleus Retrograde Response Pathway

    Directory of Open Access Journals (Sweden)

    Zhengchang Liu


    Full Text Available Intracellular communication from the mitochondria to the nucleus is achieved via the retrograde response. In budding yeast, the retrograde response, also known as the RTG pathway, is regulated positively by Rtg1, Rtg2, Rtg3 and Grr1 and negatively by Mks1, Lst8 and two 14-3-3 proteins, Bmh1/2. Activation of retrograde signaling leads to activation of Rtg1/3, two basic helix-loop-helix leucine zipper transcription factors. Rtg1/3 activation requires Rtg2, a cytoplasmic protein with an N-terminal adenosine triphosphate (ATP binding domain belonging to the actin/Hsp70/sugar kinase superfamily. The critical regulatory step of the retrograde response is the interaction between Rtg2 and Mks1. Rtg2 binds to and inactivates Mks1, allowing for activation of Rtg1/3 and the RTG pathway. When the pathway is inactive, Mks1 has dissociated from Rtg2 and bound to Bmh1/2, preventing activation of Rtg1/3. What signals association or disassociation of Mks1 and Rtg2 is unknown. Here, we show that ATP at physiological concentrations dissociates Mks1 from Rtg2 in a highly cooperative fashion. We report that ATP-mediated dissociation of Mks1 from Rtg2 is conserved in two other fungal species, K. lactis and K. waltii. Activation of Rtg1/3 upregulates expression of genes encoding enzymes catalyzing the first three reactions of the Krebs cycle, which is coupled to ATP synthesis through oxidative phosphorylation. Therefore, we propose that the retrograde response is an ATP homeostasis pathway coupling ATP production with ATP-mediated repression of the retrograde response by releasing Mks1 from Rtg2.

  17. Intrapulmonary arteries respond to serotonin and adenosine triphosphate in broiler chickens susceptible to idiopathic pulmonary arterial hypertension. (United States)

    Kluess, H A; Stafford, J; Evanson, K W; Stone, A J; Worley, J; Wideman, R F


    This study examined factors contributing to increased vascular resistance and plexiform lesion formation in broiler chickens susceptible to idiopathic pulmonary arterial hypertension (IPAH). A diet supplemented with excess tryptophan (high-Trp diet), the precursor for serotonin, was used to accelerate the development of IPAH. Broilers fed the high-Trp diet had higher pulmonary arterial pressures than broilers fed the control diet, and plexiform lesion incidences tended to be higher (P = 0.11) in the high-Trp group than in the control group at 30 d of age. The intrapulmonary arteries were assessed for vasoconstriction in response to serotonin and adenosine triphosphate (ATP) and for activities of key metabolic enzymes for serotonin and ATP. The pulmonary artery (defined as the first major branch of the pulmonary artery inside the lung) and the primary pulmonary arterial rami (defined as the second major branch of the pulmonary artery inside the lung) both exhibited vasoconstriction in response to serotonin and ATP. This is the first study to demonstrate purinergic-mediated vasoconstriction in intrapulmonary arteries from broilers. Arteriole responsiveness did not differ between broilers fed the control diet or the high-Trp diet. Therefore, the high-Trp diet enhanced the development of IPAH but did not affect the artery's sensitivity to serotonin or ATP. Monoamine oxidase activity, responsible for the breakdown of serotonin, was severely impaired in pulmonary arteries from broilers in the high-Trp group. Accordingly, serotonin may persist longer and elicit an amplified response in broilers fed the high-Trp diet.

  18. Nitrite-induced methemoglobinaemia affects blood ionized and total magnesium level by hydrolysis of plasma adenosine triphosphate in rat. (United States)

    Rahman, Md Mizanur; Kim, Shang-Jin; Kim, Gi-Beum; Hong, Chul-Un; Lee, Young-Up; Kim, Sung-Zoo; Kim, Jin-Shang; Kang, Hyung-Sub


    The objective of this study was to evaluate the effects of sodium nitrite (NaNO(2))-induced methemoglobinaemia on plasma ATP (adenosine triphosphate) and corresponding changes of blood-ionized magnesium (iMg(2+)) as well as total magnesium (tMg(2+)) in a time-dependent manner. This study was performed on male Sprague-Dawley rats to which NaNO(2) was injected (10 mg/kg i.p.) to induce methemoglobinaemia. Methemoglobin (MetHb) in blood was measured before (0 min.) and after 10, 30, 60 and 120 min. of NaNO(2) injection. At respective time points, the tMg(2+), blood ions and gases were measured by atomic absorption spectrometry and ion selective electrode, respectively. Haematological parameters were checked by automatic blood cell count, and blood films were observed under light microscope. Plasma ATP was measured by bioluminescence assay using a luminometer, and plasma proteins were measured by an automatic analyser. Blood cell count (RBC, WBC and platelet), haematocrit, and haemoglobin were found to be decreased with the advancement of MetHb concentration. With the gradual increase of MetHb concentration, the plasma ATP decreased and blood iMg(2+) and plasma tMg(2+) increased significantly as time passed by in comparison with the pre-drug values. A significant decrease of the ratio of ionized calcium to iMg(2+), Na(+) and increase of K(+) was observed. In conclusion, NaNO(2)-induced methemoglobinaemia is a cause of hydrolysis of plasma ATP which is responsible for the increase of blood iMg(2+) and plasma tMg(2+) in rats.

  19. A cascade amplification strategy based on rolling circle amplification and hydroxylamine amplified gold nanoparticles enables chemiluminescence detection of adenosine triphosphate. (United States)

    Wang, Ping; Zhang, Tonghuan; Yang, Taoyi; Jin, Nan; Zhao, Yanjun; Fan, Aiping


    A highly sensitive and selective chemiluminescent (CL) biosensor for adenosine triphosphate (ATP) was developed by taking advantage of the ATP-dependent enzymatic reaction (ATP-DER), the powerful signal amplification capability of rolling circle amplification (RCA), and hydroxylamine-amplified gold nanoparticles (Au NPs). The strategy relies on the ability of ATP, a cofactor of T4 DNA ligase, to trigger the ligation-RCA reaction. In the presence of ATP, the T4 DNA ligase catalyzes the ligation reaction between the two ends of the padlock probe, producing a closed circular DNA template that initiates the RCA reaction with phi29 DNA polymerase and dNTP. Therein, many complementary copies of the circular template can be generated. The ATP-DER is eventually converted into a detectable CL signal after a series of processes, including gold probe hybridization, hydroxylamine amplification, and oxidative gold metal dissolution coupled with a simple and sensitive luminol CL reaction. The CL signal is directly proportional to the ATP level. The results showed that the detection limit of the assay is 100 pM of ATP, which compares favorably with those of other ATP detection techniques. In addition, by taking advantage of ATP-DER, the proposed CL sensing system exhibits extraordinary specificity towards ATP and could distinguish the target molecule ATP from its analogues. The proposed method provides a new and versatile platform for the design of novel DNA ligation reaction-based CL sensing systems for other cofactors. This novel ATP-DER based CL sensing system may find wide applications in clinical diagnosis as well as in environmental and biomedical fields.

  20. Extracellular ATP Selectively Upregulates Ecto-Nucleoside Triphosphate Diphosphohydrolase 2 and Ecto-5'-Nucleotidase by Rat Cortical Astrocytes In Vitro. (United States)

    Brisevac, Dusica; Adzic, Marija; Laketa, Danijela; Parabucki, Ana; Milosevic, Milena; Lavrnja, Irena; Bjelobaba, Ivana; Sévigny, Jean; Kipp, Markus; Nedeljkovic, Nadezda


    Extracellular ATP (eATP) acts as a danger-associated molecular pattern which induces reactive response of astrocytes after brain insult, including morphological remodeling of astrocytes, proliferation, chemotaxis, and release of proinflammatory cytokines. The responses induced by eATP are under control of ecto-nucleotidases, which catalyze sequential hydrolysis of ATP to adenosine. In the mammalian brain, ecto-nucleotidases comprise three enzyme families: ecto-nucleoside triphosphate diphosphohydrolases 1-3 (NTPDase1-3), ecto-nucleotide pyrophosphatase/phospodiesterases 1-3 (NPP1-3), and ecto-5'-nucleotidase (eN), which crucially determine ATP/adenosine ratio in the pericellular milieu. Altered expression of ecto-nucleotidases has been demonstrated in several experimental models of human brain dysfunctions. In the present study, we have explored the pattern of NTPDase1-3, NPP1-3, and eN expression by cultured cortical astrocytes challenged with 1 mmol/L ATP (eATP). At the transcriptional level, eATP upregulated expression of NTPDase1, NTPDase2, NPP2, and eN, while, at translational and functional levels, these were paralleled only by the induction of NTPDase2 and eN. Additionally, eATP altered membrane topology of eN, from clusters localized in membrane domains to continuous distribution along the cell membrane. Our results suggest that eATP, by upregulating NTPDase2 and eN and altering the enzyme membrane topology, affects local kinetics of ATP metabolism and signal transduction that may have important roles in the process related to inflammation and reactive gliosis.

  1. Tunnel Waterproofing with Membranes Waterproofing at Senoko Cabele Tunnel in comparison with International Standards

    Institute of Scientific and Technical Information of China (English)

    Stefan Hotz


    Waterproofing of tunnels is and has always been a challenge, worldwide: Changing Geology with hardly predictable water inflow, limited space underground and only very little intervention opportunities once the tunnel is completed. This paper elaborates such challenges and focuses on the waterproofing solutions of mined tunnels with membranes. Different standards and approaches for the waterproofing system. In Singapore, a membrane waterproofing system is used for the shafts and adits at Senoko Cable Tunnel. The paper project specific waterproofing challenges and emphasizes on solution for them.

  2. Tunneling into quantum wires: Regularization of the tunneling Hamiltonian and consistency between free and bosonized fermions (United States)

    Filippone, Michele; Brouwer, Piet W.


    Tunneling between a point contact and a one-dimensional wire is usually described with the help of a tunneling Hamiltonian that contains a δ function in position space. Whereas the leading-order contribution to the tunneling current is independent of the way this δ function is regularized, higher-order corrections with respect to the tunneling amplitude are known to depend on the regularization. Instead of regularizing the δ function in the tunneling Hamiltonian, one may also obtain a finite tunneling current by invoking the ultraviolet cutoffs in a field-theoretic description of the electrons in the one-dimensional conductor, a procedure that is often used in the literature. For the latter case, we show that standard ultraviolet cutoffs lead to different results for the tunneling current in fermionic and bosonized formulations of the theory, when going beyond leading order in the tunneling amplitude. We show how to recover the standard fermionic result using the formalism of functional bosonization and revisit the tunneling current to leading order in the interacting case.

  3. Survey Of Wind Tunnels At Langley Research Center (United States)

    Bower, Robert E.


    Report presented at AIAA 14th Aerodynamic Testing Conference on current capabilities and planned improvements at NASA Langley Research Center's major wind tunnels. Focuses on 14 major tunnels, 8 unique in world, 3 unique in country. Covers Langley Spin Tunnel. Includes new National Transonic Facility (NTF). Also surveys Langley Unitary Plan Wind Tunnel (UPWT). Addresses resurgence of inexpensive simple-to-operate research tunnels. Predicts no shortage of tools for aerospace researcher and engineer in next decade or two.

  4. Construction of Foundation for 15-Foot Spin Tunnel (United States)


    Completed foundation for the outer housing for the 15-Foot Spin Tunnel. Charles Zimmerman was given the assignment to design and build a larger spin tunnel that would supplant the 5-foot Vertical Wind Tunnel. Authorization to build the tunnel using funds from the Federal Public Works Administration (PWA) came in June 1933. Construction started in late winter 1934 and the tunnel was operational in April 1935. The initial construction costs were $64,000.

  5. Many-electron tunneling in atoms

    CERN Document Server

    Zon, B A


    A theoretical derivation is given for the formula describing N-electron ionization of atom by a dc field and laser radiation in tunneling regime. Numerical examples are presented for noble gases atoms.

  6. Teelt van vruchtgewassen in lage tunnels

    NARCIS (Netherlands)

    Wijk, van C.A.P.; Geven, C.G.M.


    Een aantal warmteminnende vruchtgewassen zijn beproefd in lage tunnels en vergeleken met onbedekte teelt en met kasteelt. Het betrof aubergine, paprika, peper en diverse typen meloen. Daar is courgette als vergelijkend gewas aan toegevoegd

  7. Computer graphic of LHC in the tunnel

    CERN Multimedia


    A computer-generated image of the LHC particle accelerator at CERN in the tunnel originally built for the LEP accelerator that was closed in 2000. The cross-section of an LHC superconducting dipole magnet is also seen.

  8. Unknotting tunnels in hyperbolic 3-manifolds

    CERN Document Server

    Adams, Colin


    An unknotting tunnel in a 3-manifold with boundary is a properly embedded arc, the complement of an open neighborhood of which is a handlebody. A geodesic with endpoints on the cusp boundary of a hyperbolic 3-manifold and perpendicular to the cusp boundary is called a vertical geodesic. Given a vertical geodesic in a hyperbolic 3-manifold M, we find sufficient conditions for it to be an unknotting tunnel. In particular, if the vertical geodesic corresponds to a 4-bracelet, 5-bracelet or 6-bracelet in the universal cover and has short enough length, it must be an unknotting tunnel. Furthermore, we consider a vertical geodesic that satisfies the elder sibling property, which means that in the universal cover, every horoball except the one centered at infinity is connected to a larger horoball by a lift of the vertical geodesic. Such a vertical geodesic with length less than ln(2) is then shown to be an unknotting tunnel.

  9. Modeling direct interband tunneling. I. Bulk semiconductors

    Energy Technology Data Exchange (ETDEWEB)

    Pan, Andrew, E-mail: [Department of Electrical Engineering, University of California, Los Angeles, Los Angeles, California 90095 (United States); Chui, Chi On [Department of Electrical Engineering, University of California, Los Angeles, Los Angeles, California 90095 (United States); California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California 90095 (United States)


    Interband tunneling is frequently studied using the semiclassical Kane model, despite uncertainty about its validity. Revisiting the physical basis of this formula, we find that it neglects coupling to other bands and underestimates transverse tunneling. As a result, significant errors can arise at low and high fields for small and large gap materials, respectively. We derive a simple multiband tunneling model to correct these defects analytically without arbitrary parameters. Through extensive comparison with band structure and quantum transport calculations for bulk InGaAs, InAs, and InSb, we probe the accuracy of the Kane and multiband formulas and establish the superiority of the latter. We also show that the nonlocal average electric field should be used when applying either of these models to nonuniform potentials. Our findings are important for efficient analysis and simulation of bulk semiconductor devices involving tunneling.

  10. La maquette digitale du tunnel du LEP

    CERN Document Server

    Brouns, G


    Pour l'installation du LHC et son équipement périphérique (ligne cryogénique, câbles, tuyauterie, etc.) dans le tunnel du LEP, une maquette digitale CAO doit être faite. Après un rappel de la définition théorique du tunnel du LEP, cette LHC project Note décrit comment sont utilisées et intégrées d'anciennes et de nouvelles mesures du tunnel pour arriver à un ensemble de données qui permettent de construire la maquette digitale (CAO) du tunnel du LHC. Ensuite, les résultats sont comparés à leur valeur théorique d'une part, et aux anciennes valeurs disponibles d'autre part.

  11. Dielectric Sensors Based on Electromagnetic Energy Tunneling

    Directory of Open Access Journals (Sweden)

    Omar Siddiqui


    Full Text Available We show that metallic wires embedded in narrow waveguide bends and channels demonstrate resonance behavior at specific frequencies. The electromagnetic energy at these resonances tunnels through the narrow waveguide channels with almost no propagation losses. Under the tunneling behavior, high-intensity electromagnetic fields are produced in the vicinity of the metallic wires. These intense field resonances can be exploited to build highly sensitive dielectric sensors. The sensor operation is explained with the help of full-wave simulations. A practical setup consisting of a 3D waveguide bend is presented to experimentally observe the tunneling phenomenon. The tunneling frequency is predicted by determining the input impedance minima through a variational formula based on the Green function of a probe-excited parallel plate waveguide.

  12. Decoherence and tunneling of an interacting gas (United States)

    Anglin, James; Rico-Perez, Luis; Wohlfarth, Daniel


    In quasi-steady escape of a confined interacting gas by quantum tunneling, collisional decoherence can reduce the escape rate through a many-body version of the Caldeira-Leggett effect. This explains why classical fluids fail to tunnel, even though they are composed of particles small enough to be quantum mechanical. We compute this effect in the Maxwell-Boltzmann regime by deriving a quantum generalization of the Boltzmann equation. We show that decoherence effectively makes tunneling of an interacting gas into an irreversible process: a uniquely quantum mechanical form of throttling. The rate of entropy production in tunneling is related in the semi-classical limit to the imaginary part of the single-particle action.

  13. LEP sees the end of the tunnel

    CERN Multimedia


    After 14 months, which have seen the removal of 30,000 tonnes of material from the tunnel, the LEP dismantling operation has now been completed. LHC installation, which will be subject to new safety rules, can go ahead.

  14. Low Speed Wind Tunnel Facility (LSWTF) (United States)

    Federal Laboratory Consortium — Description: This facility consists of a large-scale, low-speed open-loop induction wind tunnel which has been modified to house a linear turbine cascade. A 125-hp...

  15. Hi-tech Tunnel Sets World Record

    Institute of Scientific and Technical Information of China (English)


    A sport utility vehicle takes the new direct route from Xi’an, capital city of northwest China’s Shaanxi Province, to Zhashui County through the Zhongnanshan Tunnel, which opened on January 21.With a total investment of nearly 3.2 billion yuan, the four-lane highway is the world’s longest double-tube tunnel, stretching 18.02 km.As a major part of the province’s expressway network, the tunnel connects Xi’an with southwest Shaanxi through the Qinling Mountains, shortening the previous three-hour trip to 40 minutes.The highway tunnel is also planned as part of a national highway linking north China’s Inner Mongolia Autonomous Region and south China’s Guangxi Zhuang Autonomous Region.

  16. Proton tunnelling in intermolecular hydrogen bonds

    Energy Technology Data Exchange (ETDEWEB)

    Horsewill, A.J. [Nottingham Univ. (United Kingdom); Johnson, M.R. [Institut Max von Laue - Paul Langevin (ILL), 38 - Grenoble (France); Trommsdorff, H.P. [Grenoble-1 Univ., 38 (France)


    The wavefunctions of particles extend beyond the classically accessible regions of potential energy-surfaces (PES). A manifestation of this partial delocalization is the quantum-mechanical tunneling effect which enables a particle to escape from a metastable potential-well. Tunnelling is most important for the lightest atoms, so that the determination of its contribution to proton transfer, one of the most fundamental chemical reactions, is an important issue. QENS and NMR techniques have been employed to study the motion of protons in the hydrogen bond of benzoic-acid crystals, a system which has emerged as a particularly suitable model since proton transfer occurs in a near symmetric double-well potential. The influence of quantum tunnelling was revealed and investigated in these experiments. This work provides an experimental benchmark for theoretical descriptions of translational proton-tunnelling. (author). 7 refs.

  17. Tertiary interactions within the ribosomal exit tunnel. (United States)

    Kosolapov, Andrey; Deutsch, Carol


    Although tertiary folding of whole protein domains is prohibited by the cramped dimensions of the ribosomal tunnel, dynamic tertiary interactions may permit folding of small elementary units within the tunnel. To probe this possibility, we used a beta-hairpin and an alpha-helical hairpin from the cytosolic N terminus of a voltage-gated potassium channel and determined a probability of folding for each at defined locations inside and outside the tunnel. Minimalist tertiary structures can form near the exit port of the tunnel, a region that provides an entropic window for initial exploration of local peptide conformations. Tertiary subdomains of the nascent peptide fold sequentially, but not independently, during translation. These studies offer an approach for diagnosing the molecular basis for folding defects that lead to protein malfunction and provide insight into the role of the ribosome during early potassium channel biogenesis.

  18. 7 x 10 Foot Wind Tunnel (United States)

    Federal Laboratory Consortium — This wind tunnel is used for basic and applied research in aeromechanics on advanced and unique technology rotorcraft. It supports research on advanced concepts and...

  19. Dynamical quenching of tunneling in molecular magnets

    Energy Technology Data Exchange (ETDEWEB)

    José Santander, María, E-mail: [Recursos Educativos Quántica, Santiago (Chile); Departamento de Física, Universidad de Santiago de Chile and CEDENNA, Avda. Ecuador 3493, Santiago (Chile); Nunez, Alvaro S., E-mail: [Departamento de Física, Facultad de Ciencias Físicas y Matemáticas, Universidad de Chile, Casilla 487-3, Santiago (Chile); Roldán-Molina, A. [Instituto de Física, Pontificia Universidad Católica de Valparaíso, Avenida Universidad 330, Curauma, Valparaíso (Chile); Troncoso, Roberto E., E-mail: [Centro para el Desarrollo de la Nanociencia y la Nanotecnología, CEDENNA, Avda. Ecuador 3493, Santiago 9170124 (Chile); Departamento de Física, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso (Chile)


    It is shown that a single molecular magnet placed in a rapidly oscillating magnetic field displays the phenomenon of quenching of tunneling processes. The results open a way to manipulate the quantum states of molecular magnets by means of radiation in the terahertz range. Our analysis separates the time evolution into slow and fast components thereby obtaining an effective theory for the slow dynamics. This effective theory presents quenching of the tunnel effect, in particular, stands out its difference with the so-called coherent destruction of tunneling. We support our prediction with numerical evidence based on an exact solution of Schrödinger's equation. - Highlights: • Single molecular magnets under rapidly oscillating magnetic fields is studied. • It is shown that this system displays the quenching of tunneling processes. • Our findings provide a control of quantum molecular magnets via terahertz radiation.

  20. Computational Multiqubit Tunnelling in Programmable Quantum Annealers (United States)


    classical simulated annealing6 that aims to take advantage of quantum tunnelling. In classical cooling optimization algorithms such as simulated have established a quantum speedup. To this end, one would have to demonstrate that no known classical algorithm finds the optimal solution as fast...classical algorithms such as Quantum Monte Carlo or by employing cluster update methods. However, the collective tunnelling phenomena demonstrated here

  1. Low Power Band to Band Tunnel Transistors (United States)


    the E-field and tunneling at the source- pocket junction you form a parasitic NPN + transistor and the injection mechanism of carriers into the...hypothesis that the 1000 ° C, 5s anneal split lead to a very wide pocket and the accidental formation of a NPN + transistor , while the 1000 ° C, 1s anneal...Low Power Band to Band Tunnel Transistors Anupama Bowonder Electrical Engineering and Computer Sciences University of California at Berkeley

  2. Aeroelastic instability stoppers for wind tunnel models (United States)

    Doggett, R. V., Jr.; Ricketts, R. H. (Inventor)


    A mechanism for constraining models or sections thereof, was wind tunnel tested, deployed at the onset of aeroelastic instability, to forestall destructive vibrations in the model is described. The mechanism includes a pair of arms pivoted to the tunnel wall and straddling the model. Rollers on the ends of the arms contact the model, and are pulled together against the model by a spring stretched between the arms. An actuator mechanism swings the arms into place and back as desired.

  3. Geology of Pletovarje motorway tunnel (Slovenia

    Directory of Open Access Journals (Sweden)

    Magda Čarman


    Full Text Available Styrian motorway crosses the Pletovarje ridge through the tunnel having the same name. It intersects the mosteastern extension of the Southern Karavanke tectonic unit. Considering geological conditions, the tunnel couldbe divided into three sections: the inner Donat / Dona~ka fault zone composed of tectonic lenses of differentlithostratigraphic units (of paleozoic, triassic and tertiary age, massive dolomite (lower triassic and Smrekovecseries of oligocene age. Main faults have east – west direction and are subvertical.

  4. The bacterial lipocalins. (United States)

    Bishop, R E


    The lipocalins were once regarded as a eukaryotic protein family, but new members have been recently discovered in bacteria. The first bacterial lipocalin (Blc) was identified in Escherichia coli as an outer membrane lipoprotein expressed under conditions of environmental stress. Blc is distinguished from most lipocalins by the absence of intramolecular disulfide bonds, but the presence of a membrane anchor is shared with two of its closest homologues, apolipoprotein D and lazarillo. Several common features of the membrane-anchored lipocalins suggest that each may play an important role in membrane biogenesis and repair. Additionally, Blc proteins are implicated in the dissemination of antibiotic resistance genes and in the activation of immunity. Recent genome sequencing efforts reveal the existence of at least 20 bacterial lipocalins. The lipocalins appear to have originated in Gram-negative bacteria and were probably transferred horizontally to eukaryotes from the endosymbiotic alpha-proteobacterial ancestor of the mitochondrion. The genome sequences also reveal that some bacterial lipocalins exhibit disulfide bonds and alternative modes of subcellular localization, which include targeting to the periplasmic space, the cytoplasmic membrane, and the cytosol. The relationships between bacterial lipocalin structure and function further illuminate the common biochemistry of bacterial and eukaryotic cells.

  5. Radiometric dating of the Siloam Tunnel, Jerusalem. (United States)

    Frumkin, Amos; Shimron, Aryeh; Rosenbaum, Jeff


    The historical credibility of texts from the Bible is often debated when compared with Iron Age archaeological finds (refs. 1, 2 and references therein). Modern scientific methods may, in principle, be used to independently date structures that seem to be mentioned in the biblical text, to evaluate its historical authenticity. In reality, however, this approach is extremely difficult because of poor archaeological preservation, uncertainty in identification, scarcity of datable materials, and restricted scientific access into well-identified worship sites. Because of these problems, no well-identified Biblical structure has been radiometrically dated until now. Here we report radiocarbon and U-Th dating of the Siloam Tunnel, proving its Iron Age II date; we conclude that the Biblical text presents an accurate historic record of the Siloam Tunnel's construction. Being one of the longest ancient water tunnels lacking intermediate shafts, dating the Siloam Tunnel is a key to determining where and when this technological breakthrough took place. Siloam Tunnel dating also refutes a claim that the tunnel was constructed in the second century bc.

  6. Some Caves in tunnels in Dinaric karst of Croatia (United States)

    Garasic, Mladen; Garasic, Davor


    In the last 50 years during the construction of almost all the tunnels in the Croatian Dinaric Karst thousands of caves have been encountered that represented the major problems during the construction works. Geological features (fissures, folding, faults, etc.) are described in this contribution, together with the hydrogeological conditions (rapid changes in groundwater levels). Special engineering geological exploration and survey of each cave, together with the stabilization of the tunnel ceiling, and groundwater protection actions according to basic engineering geological parameters are also presented. In karst tunneling in Croatia over 150 caves longer than 500 m have been investigated. Several caves are over 300 m deep (St. Ilija tunnel in Biokovo Mt), and 10 are longer than 1000 m (St.Rok tunnel, HE Senj and HE Velebit tunnels in Velebit Mt, Ucka tunnel in Ucka Mt, Mala kapela tunnel in Kapela Mt, caverns in HE Plat tunnel etc). Different solutions were chosen to cross the caves depending on the size and purpose of the tunnels (road, rail, pedestrian tunnel, or hydrotechnical tunnels). This is presentations of interesting examples of ceiling stabilization in big cave chambers, construction of bridges inside tunnels, deviations of tunnels, filling caves, grouting, etc. A complex type of karstification has been found in the cavern at the contact between the Palaeozoic clastic impervious formations and the Mesozoic complex of dolomitic limestones in the Vrata Tunnel and at the contact with flysch in the Učka Tunnel. However, karstification advancing in all directions at a similar rate is quite rare. The need to have the roadway and/or tunnel above water from a spring is the biggest possible engineering-geological, hydrogeological and civil engineering challenge. Significant examples are those above the Jadro spring (Mravinci tunnel) in flysch materials or above the Zvir spring in Rijeka (Katarina tunnel), and in fractured Mesozoic carbonates. Today in Croatian

  7. Le LHC, un tunnel cosmique

    CERN Document Server

    CERN. Geneva


    Et si la lumière au bout du tunnel du LHC était cosmique ? En d’autres termes, qu’est-ce que le LHC peut nous apporter dans la connaissance de l’Univers ? Car la montée en énergie des accélérateurs de particules nous permet de mieux appréhender l’univers primordial, chaud et dense. Mais dans quel sens dit-on que le LHC reproduit des conditions proches du Big bang ? Quelles informations nous apporte-t-il sur le contenu de l’Univers ? La matière noire est-elle détectable au LHC ? L’énergie noire ? Pourquoi l’antimatière accumulée au CERN est-elle si rare dans l’Univers ? Et si le CERN a bâti sa réputation sur l’exploration des forces faibles et fortes qui opèrent au sein des atomes et de leurs noyaux, est-ce que le LHC peut nous apporter des informations sur la force gravitationnelle qui gouverne l’évolution cosmique ? Depuis une trentaine d’années, notre compréhension de l’univers dans ses plus grandes dimensions et l’appréhension de son comportement aux plus peti...

  8. Tunneling and Transport in Nanowires

    Energy Technology Data Exchange (ETDEWEB)

    Goldman, Allen M. [Univ. of Minnesota, Minneapolis, MN (United States)


    The goal of this program was to study new physical phenomena that might be relevant to the performance of conductive devices and circuits of the smallest realizable feature sizes possible using physical rather than biological techniques. Although the initial scientific work supported involved the use of scanning tunneling microscopy and spectroscopy to ascertain the statistics of the energy level distribution of randomly sized and randomly shaped quantum dots, or nano-crystals, the main focus was on the investigation of selected properties, including superconductivity, of conducting and superconducting nanowires prepared using electron-beam-lithography. We discovered a magnetic-field-restoration of superconductivity in out-of-equilibrium nanowires driven resistive by current. This phenomenon was explained by the existence of a state in which dissipation coexisted with nonvanishing superconducting order. We also produced ultra-small superconducting loops to study a predicted anomalous fluxoid quantization, but instead, found a magnetic-field-dependent, high-resistance state, rather than superconductivity. Finally, we developed a simple and controllable nanowire in an induced charged layer near the surface of a masked single-crystal insulator, SrTiO3. The layer was induced using an electric double layer transistor employing an ionic liquid (IL). The transport properties of the induced nanowire resembled those of collective electronic transport through an array of quantum dots.

  9. Tunneling and Transport in Nanowires

    Energy Technology Data Exchange (ETDEWEB)

    Goldman, Allen M. [Univ. of Minnesota, Minneapolis, MN (United States)


    The goal of this program was to study new physical phenomena that might be relevant to the performance of conductive devices and circuits of the smallest realizable feature sizes possible using physical rather than biological techniques. Although the initial scientific work supported involved the use of scanning tunneling microscopy and spectroscopy to ascertain the statistics of the energy level distribution of randomly sized and randomly shaped quantum dots, or nano-crystals, the main focus was on the investigation of selected properties, including superconductivity, of conducting and superconducting nanowires prepared using electron-beam-lithography. We discovered a magnetic-field-restoration of superconductivity in out-of-equilibrium nanowires driven resistive by current. This phenomenon was explained by the existence of a state in which dissipation coexisted with nonvanishing superconducting order. We also produced ultra-small superconducting loops to study a predicted anomalous fluxoid quantization, but instead, found a magnetic-field-dependent, high-resistance state, rather than superconductivity. Finally, we developed a simple and controllable nanowire in an induced charged layer near the surface of a masked single-crystal insulator, SrTiO3. The layer was induced using an electric double layer transistor employing an ionic liquid (IL). The transport properties of the induced nanowire resembled those of collective electronic transport through an array of quantum dots.

  10. Study on calculation of rock pressure for ultra-shallow tunnel in poor surrounding rock and its tunneling procedure

    Institute of Scientific and Technical Information of China (English)

    Xiaojun Zhou; Jinghe Wang; Bentao Lin


    A computational method of rock pressure applied to an ultra-shallow tunnel is presented by key block theory, and its mathematical formula is proposed according to a mechanical tunnel model with super-shallow depth. Theoretical analysis shows that the tunnel is subject to asymmetric rock pressure due to oblique topography. The rock pressure applied to the tunnel crown and sidewall is closely related to the surrounding rock bulk density, tunnel size, depth and angle of oblique ground slope. The rock pressure applied to the tunnel crown is much greater than that to the sidewalls, and the load applied to the left side-wall is also greater than that to the right sidewall. Mean-while, the safety of the lining for an ultra-shallow tunnel in strata with inclined surface is affected by rock pressure and tunnel support parameters. Steel pipe grouting from ground surface is used to consolidate the unfavorable surrounding rock before tunnel excavation, and the reinforcing scope is proposed according to the analysis of the asymmetric load induced by tunnel excavation in weak rock with inclined ground surface. The tunneling procedure of bench cut method with pipe roof protection is still discussed and carried out in this paper according to the special geological condition. The method and tunneling procedure have been successfully utilized to design and drive a real expressway tunnel. The practice in building the super-shallow tunnel has proved the feasibility of the calculation method and tunneling procedure presented in this paper.

  11. Electron transfer pathway analysis in bacterial photosynthetic reaction center

    CERN Document Server

    Kitoh-Nishioka, Hirotaka


    A new computational scheme to analyze electron transfer (ET) pathways in large biomolecules is presented with applications to ETs in bacterial photosynthetic reaction center. It consists of a linear combination of fragment molecular orbitals and an electron tunneling current analysis, which enables an efficient first-principles analysis of ET pathways in large biomolecules. The scheme has been applied to the ET from menaquinone to ubiquinone via nonheme iron complex in bacterial photosynthetic reaction center. It has revealed that not only the central Fe$^{2+}$ ion but also particular histidine ligands are involved in the ET pathways in such a way to mitigate perturbations that can be caused by metal ion substitution and depletion, which elucidates the experimentally observed insensitivity of the ET rate to these perturbations.

  12. Bacterial glycosyltransferase toxins. (United States)

    Jank, Thomas; Belyi, Yury; Aktories, Klaus


    Mono-glycosylation of host proteins is a common mechanism by which bacterial protein toxins manipulate cellular functions of eukaryotic target host cells. Prototypic for this group of glycosyltransferase toxins are Clostridium difficile toxins A and B, which modify guanine nucleotide-binding proteins of the Rho family. However, toxin-induced glycosylation is not restricted to the Clostridia. Various types of bacterial pathogens including Escherichia coli, Yersinia, Photorhabdus and Legionella species produce glycosyltransferase toxins. Recent studies discovered novel unexpected variations in host protein targets and amino acid acceptors of toxin-catalysed glycosylation. These findings open new perspectives in toxin as well as in carbohydrate research.

  13. Type Ⅰ inositol 1,4,5-triphosphate receptors increase in kidney of mice with fulminant hepatic failure

    Institute of Scientific and Technical Information of China (English)

    Ying Wen; Wei Cui; Pei Liu


    AIM:To delineate the mechanisms of renal vasoconstriction in hepatorenal syndrome (HRS),we investigated the expression of type Ⅰ inositol 1,4,5-triphosphate receptors (IP3R Ⅰ) of kidney in mice with fulminant hepatic failure (FHF).METHODS:FHF was induced by lipopolysaccharide (LPS) in D-galactosamine (GaIN) sensitized BALB/c mice.There were 20 mice in normal saline (NS)-treated group,20 mice in LPS-treated group,20 mice in GaINtreated group,and 60 mice in GaIN/LPS-treated group (FHF group).Liver and kidney tissues were obtained at 2,6,and 9 h after administration.The liver and kidney specimens were stained with hematoxylin-eosin for studying morphological changes under light microscope.The expression of IP3R Ⅰ in kidney tissue was tested by immunohistochemistry,Western blot and reverse transcription (RT)-PCR.RESULTS:Kidney tissues were morphologically normal at all time points in all groups.IP3R Ⅰ proteins were found localized in the plasma region of glomerular mesangial cells (GMC) and vascular smooth muscle cells (VSMC) in kidney by immunohistochemical staining.In kidney of mice with FHF at 6 h and 9 h IP3R Ⅰ staining was upregulated.Results from Western blot demonstrated consistent and significant increment of IP3R Ⅰ expression in mice with FHF at 6 h and 9 h (t = 3.16,P<0.05;t = 5.43,P<0.01).Furthermore,we evaluated IP3R Ⅰ mRNA expression by RT-PCR and observed marked upregulation of IP3R Ⅰ mRNA in FHF samples at 2 h,6 h and 9 h compared to controls (t = 2.97,P<0.05;t = 4.42,P<0.01;t = 3.81,P<0.01).CONCLUSION:The expression of IP3R Ⅰ protein increased in GMC and renal VSMC of mice with FHF,possibly caused by up-regulation of IP3R Ⅰ mRNA.

  14. Quantum dot resonant tunneling diode single photon detector with aluminum oxide aperture defined tunneling area

    DEFF Research Database (Denmark)

    Li, H.W.; Kardynal, Beata; Ellis, D.J.P.;


    Quantum dot resonant tunneling diode single photon detector with independently defined absorption and sensing areas is demonstrated. The device, in which the tunneling is constricted to an aperture in an insulating layer in the emitter, shows electrical characteristics typical of high quality res...

  15. European Tunnelling Review Quality, Environment and Safety Focus

    Institute of Scientific and Technical Information of China (English)

    Bill Weburn


    This paper provides an overview of tunnelling projects with some of the themes covered by the Chengdu Tunnelling Conference.(a) Environmental issues and tunnels under high groundwater pressure (Hallandsis Rail Tunnel, Sweden)(b) Fire -fighting and tunnel safety on long highway tunnels (Socatop Tunnel, West Paris)(c) Mechanised tunnelling below the world's largest airport (Heathrow T5) of a quality that avoids disruption to its operation.Each of these overviews show an enhanced performance in regard to project specific events or objectives. Particularly in tunnelling, project owners expect more than just a facility delivered on time for their money: they expect an all round performance respecting deadlines, budget, environmental protection, safety, quality and community harmony. Funding agencies do not always have the same agenda as project owners, acknowledging peripheral selection criteria but often demanding that the lowest bidder be awarded work. All round performance requires all round project selection criteria.

  16. Development of the tunneling junction simulation environment for scanning tunneling microscope evaluation (United States)

    Gajewski, Krzysztof; Piasecki, Tomasz; Kopiec, Daniel; Gotszalk, Teodor


    Proper configuration of scanning tunneling microscope electronics plays an important role in the atomic scale resolution surface imaging. Device evaluation in the tunneling contact between scanning tip and sample may be prone to the surface quality or mechanical disturbances. Thus the use of tunneling junction simulator makes electronics testing more reliable and increases its repeatability. Here, we present the theoretical background enabling the proper selection of electronic components circuitry used as a tunneling junction simulator. We also show how to simulate mechanics related to the piezoelectric scanner, which is applied in real experiments. Practical use of the proposed simulator and its application in metrological characterization of the developed scanning tunneling microscope is also shown.

  17. Seizures Complicating Bacterial Meningitis

    Directory of Open Access Journals (Sweden)

    J Gordon Millichap


    Full Text Available The clinical data of 116 patients, 1 month to <5 years of age, admitted for bacterial meningitis, and grouped according to those with and without seizures during hospitalization, were compared in a study at Buddhist Dalin Tzu Chi General Hospital, Chang Gung Memorial Hospital and other centers in Taiwan.

  18. Determination of the effectiveness of concentrating mine-tunneling operations

    Energy Technology Data Exchange (ETDEWEB)

    Domanov, P.I.


    Experimental operations were conducted in 163 tunneling faces equipped with loading machines and mounted drilling machines and in 110 faces equipped with the pneumatic drilling machines 1PNB-2E in order to determine the optimal specific population of tunnellers in a working face. Following computerized processing of the obtained data, mathematical formulas were derived to compute the optimal rate of tunneling drifts with respect to productivity. Relationships were given for the productivity of drift miners and the rate of tunneling drifts, and between the complete cost of tunneling a drift and the velocity of tunneling. 3 figures.

  19. Performance Analysis of VOIP over GRE Tunnel

    Directory of Open Access Journals (Sweden)

    Aria. Asadi Eskandar


    Full Text Available Voice over IP (VoIP is commonly known as phone service over the Internet. Any service using public IP network requires certain extent of security. Demand for security in VOIP technology is increasing. VPN is one of the commonly used methods to secure VOIP traffic. In this paper we simulated behavior of a VOIP communication while running over a GRE VPN Tunnel using OPNET Modeler 17.5. During the simulation, such performance parameters as: choice of voice signaling protocol, voice Codec, parameters arising from network QoS (in this study, homogenous vs. heterogeneous network environment and type of VPN tunneling protocol, were examined. We evaluated performance of VOIP communications in homogenous and heterogeneous network environments, configured based on two different signaling protocols, namely H.323 and SIP. Also, G.711 and G.723 were configured and tested as the choice for voice Codecs. GRE was implemented as the tunneling protocol. Result analysis of this study indicated that GRE Tunnel didn't show a significant increase in such call quality of service (QoS performance factors as: end-to-end delay, call setup time, or a decrease in call MOS value. Even though in a non-ideal (heterogeneous network environment, call quality of service (QoS performance factors shoed poor results; however, there was no significant evidence to suggest that GRE Tunnel is the root cause for such poor results.

  20. Tunnel Field-Effect Transistor with Epitaxially Grown Tunnel Junction Fabricated by Source/Drain-First and Tunnel-Junction-Last Processes (United States)

    Morita, Yukinori; Mori, Takahiro; Migita, Shinji; Mizubayashi, Wataru; Tanabe, Akihito; Fukuda, Koichi; Masahara, Meishoku; Ota, Hiroyuki


    We fabricate p- and n-channel Si tunnel field-effect transistors (TFETs) with an epitaxially grown tunnel junction. In a novel source/drain-first and tunnel-junction-last fabrication process, a thin epitaxial undoped Si channel (epichannel) is deposited on a preferentially fabricated p- or n-type source area. The epichannel sandwiched by a gate insulator and a highly doped source well acts as a parallel-plate tunnel capacitor, which effectively multiplies drain current with an enlarged tunnel area. On the basis of its simple structure and easy fabrication, symmetric n- and p-transistor and complementary metal oxide semiconductor inverter operations were successfully demonstrated.

  1. Overview of traffic safety aspects and design in road tunnels

    Directory of Open Access Journals (Sweden)

    Shy Bassan


    Drivers in road tunnels generally reduce their speed and increase their lateral position from the right tunnel wall while driving. In shorter tunnels, with reduced driving speed, driver vigilance may be more robust without being hindered by dull driving, which is more common in longer tunnels. Still, in spite of driver alertness, crash rates in tunnels occur due to the tunnel's unusual driving environment. Crash rates are lower in the tunnel inner zone due to driver alertness, especially after passing the transition zone and acclimating to the tunnel environment. The number of crashes, however, is higher along zone 4 (tunnel inner zone, which is the principal zone, as it covers longer driving distance. According to most studies, short tunnels were found to exhibit higher crash rates than long tunnels because the entrance zones incorporate higher crash rates, compared with the midzones; nonetheless, longer unidirectional (freeway and multilane tunnels with higher design speed, entail lower driver alertness and diminished concentration due to relatively monotonous driving in spite of a tunnel's closed environment.

  2. Disposable bioluminescence-based biosensor for detection of bacterial count in food. (United States)

    Luo, Jinping; Liu, Xiaohong; Tian, Qing; Yue, Weiwei; Zeng, Jing; Chen, Guangquan; Cai, Xinxia


    A biosensor for rapid detection of bacterial count based on adenosine 5'-triphosphate (ATP) bioluminescence has been developed. The biosensor is composed of a key sensitive element and a photomultiplier tube used as a detector element. The disposable sensitive element consists of a sampler, a cartridge where intracellular ATP is chemically extracted from bacteria, and a microtube where the extracted ATP reacts with the luciferin-luciferase reagent to produce bioluminescence. The bioluminescence signal is transformed into relevant electrical signal by the detector and further measured with a homemade luminometer. Parameters affecting the amount of the extracted ATP, including the types of ATP extractants, the concentrations of ATP extractant, and the relevant neutralizing reagent, were optimized. Under the optimal experimental conditions, the biosensor showed a linear response to standard bacteria in a concentration range from 10(3) to 10(8) colony-forming units (CFU) per milliliter with a correlation coefficient of 0.925 (n=22) within 5min. Moreover, the bacterial count of real food samples obtained by the biosensor correlated well with those by the conventional plate count method. The proposed biosensor, with characteristics of low cost, easy operation, and fast response, provides potential application to rapid evaluation of bacterial contamination in the food industry, environment monitoring, and other fields.

  3. Simple, Fast and Selective Detection of Adenosine Triphosphate at Physiological pH Using Unmodified Gold Nanoparticles as Colorimetric Probes and Metal Ions as Cross-Linkers

    Directory of Open Access Journals (Sweden)

    Huan Pang


    Full Text Available We report a simple, fast and selective colorimetric assay of adenosine triphosphate (ATP using unmodified gold nanoparticles (AuNPs as probes and metal ions as cross-linkers. ATP can be assembled onto the surface of AuNPs through interaction between the electron-rich nitrogen atoms and the electron-deficient surface of AuNPs. Accordingly, Cu2+ ions induce a change in the color and UV/Vis absorbance of AuNPs by coordinating to the triphosphate groups and a ring nitrogen of ATP. A detection limit of 50 nM was achieved, which is comparable to or lower than that achievable by the currently used electrochemical, spectroscopic or chromatographic methods. The theoretical simplicity and high selectivity reported herein demonstrated that AuNPs-based colorimetric assay could be applied in a wide variety of fields by rationally designing the surface chemistry of AuNPs. In addition, our results indicate that ATP-modified AuNPs are less stable in Cu2+, Cd2+ or Zn2+-containing solutions due to the formation of the corresponding dimeric metal-ATP complexes.

  4. Diadenosine triphosphate is a novel factor which in combination with cyclodextrins synergistically enhances the biosynthesis of trans-resveratrol in Vitis vinifera cv. Monastrell suspension cultured cells. (United States)

    Pietrowska-Borek, Małgorzata; Czekała, Lukasz; Belchí-Navarro, Sarai; Pedreño, María Angeles; Guranowski, Andrzej


    Dinucleoside polyphosphates are considered as signal molecules that may evoke response of plant cells to stress. Other compounds whose biological effects have been recognized are cyclodextrins. They are cyclic oligosaccharides that chemically resemble the alkyl-derived pectic oligosaccharides naturally released from the cell walls during fungal attack, and they act as true elicitors, since, when added to plant cell culture, they induce the expression of genes involved in some secondary metabolism pathways. Previously, we demonstrated that some dinucleoside polyphosphates triggered the biosynthesis of enzymes involved in the phenylpropanoid pathway in Arabidopsis thaliana. In Vitis vinifera suspension cultured cells, cyclodextrins were shown to enhance the accumulation of trans-resveratrol, one of the basic units of the stilbenes derived from the phenylpropanoid pathway. Here, we show that diadenosine triphosphate, applied alone or in combination with cyclodextrins to the grapevine suspension-cultured cells, increased the transcript level of genes encoding key phenylpropanoid-pathway enzymes as well as the trans-resveratrol production inside cells and its secretion into the extracellular medium. In the latter case, these two compounds acted synergistically. However, the accumulation of trans-resveratrol and its glucoside trans-piceid inside cells were stimulated much better by diadenosine triphosphate than by cyclodextrins.

  5. Molecular characterization of a dual inhibitory and mutagenic activity of 5-fluorouridine triphosphate on viral RNA synthesis. Implications for lethal mutagenesis. (United States)

    Agudo, Rubén; Arias, Armando; Pariente, Nonia; Perales, Celia; Escarmís, Cristina; Jorge, Alberto; Marina, Anabel; Domingo, Esteban


    The basis for a dual inhibitory and mutagenic activity of 5-fluorouracil (5-FU) on foot-and-mouth disease virus (FMDV) RNA replication has been investigated with purified viral RNA-dependent RNA polymerase (3D) in vitro. 5-Fluorouridine triphosphate acted as a potent competitive inhibitor of VPg uridylylation, the initial step of viral replication. Peptide analysis by mass spectrometry has identified a VPg fragment containing 5-fluorouridine monophosphate (FUMP) covalently attached to Tyr3, the amino acid target of the uridylylation reaction. During RNA elongation, FUMP was incorporated in the place of UMP or CMP by FMDV 3D, using homopolymeric and heteropolymeric templates. Incorporation of FUMP did not prevent chain elongation, and, in some sequence contexts, it favored misincorporations at downstream positions. When present in the template, FUMP directed the incorporation of AMP and GMP, with ATP being a more effective substrate than GTP. The misincorporation of GMP was 17-fold faster opposite FU than opposite U in the template. These results in vitro are consistent with the mutational bias observed in the mutant spectra of 5-FU-treated FMDV populations. The dual mutagenic and inhibitory activity of 5-fluorouridine triphosphate may contribute to the effective extinction of FMDV by 5-FU through virus entry into error catastrophe.

  6. Biological effects of exogenous adenosine 5 prime -triphosphate on cultured mammalian cells: Evidence for a receptor mechanism and its regulation by desensitization

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez, F.A.


    Exogenous adenosine 5{prime}-triphosphate (ATP) mobilized intracellular calcium in human carcinoma A43l cells and in Swiss 3T3 and 3T6 mouse fibroblasts by increasing inositol trisphosphate similar to well down growth factors (platelet-derived growth factor (PDGF), epidermal growth factor (EGF), bradykinin (BK), serum). Calcium mobilization was examined by video imaging of fura-2 fluorescence is single cells, following the radioactive isotope {sup 45}Ca, and monitoring the decrease in fluorescence of cells loaded with chlortetracycline. Uridine 5{prime}-triphosphate, but not other nucleotides, mimicked ATP. Single-cell analysis revealed synchronous responses in 10 sec to ATP, BK or serum, while PDGF (3T3) and EGF (A431) produced slower signals with significant cell-to-cell variation. PDGF desensitized 3T3 cells to ATP and BK added 100 sec later but ATP or BK did not desensitized to PDGF. Homologous desensitization was seen with all agonists. Heterologous desensitization was also observed in A431 cells where ATP desensitized to serum, but serum did not to ATP. ATP-stimulated calcium entry was detected after 10 sec in A431 cells, but not in Swiss 3T6 cells. Entry started before significant efflux had occurred and did not fit the capacitance model of Putney. A 2-3 hr ATP pretreatment produced a homologous desensitization state that required 20 hr to disappear, probably due to down-regulation of the putative ATP receptors.

  7. Isolation of a gene encoding a developmentally regulated T cell-specific protein with a guanine nucleotide triphosphate-binding motif

    Energy Technology Data Exchange (ETDEWEB)

    Carlow, D.A.; Teh, H.S.; Marth, J. [Univ. of British Columbia, Vancouver (Canada)] [and others


    In this study, we describe a novel full length cDNA clone designated Tgtp that encodes a predicted 415-amino acid a T cell-specific guanine nucleotide triphosphate-binding protein (TGTP) bearing the characteristic motifs of a guanine nucleotide triphosphate (GTP) binding protein. Tgtp is expressed preferentially, if not exclusively, in T cells, and is up-regulated in both unfractionated and in purified CD4{sup +}8{sup +} thymocytes upon TCR cross-linking. In contrast, expression of Tgtp in peripheral T cells is maintained at relatively high levels and is not grossly affected by TCR cross-linking. Antiserum generated against synthetic peptides from the predicted TGTP amino acid sequence recognized a single protein with a molecular mass of {approx}50 kDa, corresponding well with the computed molecular mass of 47 kDa. The only known relative of Tgtp is MUSGTP, which is reportedly expressed in B cells and bears a GTP binding motif. Thus, the discovery of Tgtp resolves a subfamily of molecules with GTP binding motifs and apparent lymphoid lineage-restricted expression. Given the restricted expression pattern in T cells, the up-regulated expression observed in response to TCR signaling in immature thymocytes, and the presence of the motifs characteristic of GTP binding proteins, we suggest that TGTP may have an important function in T cell development and/or T cell activation. 51 refs., 6 figs.

  8. 三聚磷酸铝缓蚀性能的EIS研究%Electrochemical Impedance Spectroscopy Studies on Inhibitive Performance of Aluminum Triphosphate Pigment

    Institute of Scientific and Technical Information of China (English)

    廖永贵; 辜志俊; 张志刚; 郭琦龙; 苏方腾


    The epoxy coatings on A3 steel sheets containing calcite or aluminum triphosphate (AlTP), were investigated by Electrochemical Impedance Spectroscopy (EIS). The results showed that calcite pigment is not an inhibitor, but an extender pigment, and that AlTP pigment can considerably enhance the protective performance of epoxy coating. The triphosphate ion at the interface between the steel substrate and the coating can complex with the corrosion-product, such as ferric and ferrous ions, and form a compact protective film which effectively separates the steel substrate from the aggressive media.%利用电化学阻抗谱研究了方解石及三聚磷酸铝环氧涂层的耐蚀性能,并提出了它们的作用机理.结果表明,方解石只起体质颜料作用,不具备缓蚀性能;而三聚磷酸铝因在钢基表面作用形成致密的保护膜则表现出优良的缓蚀性能.

  9. Radioimmunoassay for guanosin-5'-diphosphate-3'-diphosphate and adenosine-5'-triphosphate-3'-diphosphate

    Energy Technology Data Exchange (ETDEWEB)

    Hamagishi, Y.; Oki, T.; Tone, H.; Inui, T. (Sanraku-Ocean Co. Ltd., Fujisawa, Kanagawa (Japan). Central Research Lab.)


    A radioimmunoassay for guanosine-5'-diphosphate-3'-diphosphate (ppGpp) and adenosine-5'-triphosphate-3'-diphosphate (pppApp) has been developed. The assay method is based on competition of an unlabeled highly phosphorylated nucleotide with /sup 3/H-labeled highly phosphorylated nucleotide for binding sites on a specific antibody. Antibodies to ppGpp and pppApp were obtained by immunizing rabbits with the antigen prepared by conjugating ppGpp with human serum albumin using 1-ethyl-3-(3-dimethylaminoprophyl)carbodiimide, and with the antigen prepared by conjugating 8-(6-aminohexyl)amino-adenosine-5'-triphosphate-3'-diphosphate with human serum albumin using glutaraldehyde, respectively. Antibody-bound /sup 3/H-labeled highly phosphorylated nucleotides were separated from the free /sup 3/H-labeled highly phosphorylated nucleotides by selective adsorption on dextran-coated charcoal. Displacement plots were linear over a concentration range of 5 - 1,000 pmol/assay tube in a log-probit percentage plot. Application of this method to biological systems offers improved accuracy and convenience compared with the previous /sup 32/PO/sub 4/-labeling technique.

  10. Multidimensional Quantum Tunneling in the Schwinger Effect

    CERN Document Server

    Dumlu, Cesim K


    We study the Schwinger effect, in which the external field having a spatio-temporal profile creates electron-positron pairs via multidimensional quantum tunneling. Our treatment is based on Gutzwiller's trace formula for the QED effective action, whose imaginary part is represented by a sum over complex wordlines. The worldlines are multi-periodic, and the periods of motion collectively depend on the strength of spatial and temporal inhomogeneity. We argue that Hamilton's characteristic function that leads to the correct tunneling amplitude must explicitly depend on both periods, and is represented by an average over the internal cycles of motion. We use this averaging method to calculate the pair production rate in an exponentially damped sinusoidal field, where we find that the initial conditions for each family of periodic trajectories lie on a curve in the momentum plane. The ratio of the periods, which may also be referred as the topological index, stays uniform on each curve. Calculation of tunneling am...

  11. Positioning systems for underground tunnel environments

    CERN Document Server

    Leite Pereira, Fernando; Theis, Christian

    In the last years the world has witnessed a remarkable change in the computing concept by entering the mobile era. Incredibly powerful smartphones have proliferated at stunning pace and tablet computers are capable of running demanding applications and meet new business requirements. Being wireless, localization has become crucial not only to serve individuals but also help companies in industrial and safety processes. In the context of the Radiation Protection group at CERN, automatic localization, besides allowing to find people, would help improving the radiation surveys performed regularly along the accelerator tunnels. The research presented in this thesis attempts to answer questions relatively to the viability of localization in a harsh conditions tunnel: “Is localization in a very long tunnel possible, meeting its restrictions and without incurring prohibitive costs and infrastructure?”, “Can one achieve meter-level accuracy with GSM deployed over leaky-feeder?”, “Is it possible to prototype...

  12. Electronic thermometry in tunable tunnel junction

    Energy Technology Data Exchange (ETDEWEB)

    Maksymovych, Petro


    A tunable tunnel junction thermometry circuit includes a variable width tunnel junction between a test object and a probe. The junction width is varied and a change in thermovoltage across the junction with respect to the change in distance across the junction is determined. Also, a change in biased current with respect to a change in distance across the junction is determined. A temperature gradient across the junction is determined based on a mathematical relationship between the temperature gradient, the change in thermovoltage with respect to distance and the change in biased current with respect to distance. Thermovoltage may be measured by nullifying a thermoelectric tunneling current with an applied voltage supply level. A piezoelectric actuator may modulate the probe, and thus the junction width, to vary thermovoltage and biased current across the junction. Lock-in amplifiers measure the derivatives of the thermovoltage and biased current modulated by varying junction width.

  13. Vibration Compensation for Scanning Tunneling Microscope

    Institute of Scientific and Technical Information of China (English)

    LI Meng-chao; FU Xing; WEI Xiao-lei; HU Xiao-tang


    The influence of vibration is already one of main obstacles for improving the nano measuring accuracy.The techniques of anti-vibration,vibration isolation and vibration compensation become an important branch in nano measuring field.Starting with the research of sensitivity to vibration of scanning tunneling microscope(STM),the theory,techniques and realization methods of nano vibration sensor based on tunnel effect are initially investigated,followed by developing the experimental devices.The experiments of the vibration detection and vibration compensation are carried out.The experimental results show that vibration sensor based on tunnel effect is characterized by high sensitivity,good frequency characteristic and the same vibratory response characteristic consistent with STM.

  14. Isotope effects of hydrogen and atom tunnelling (United States)

    Buchachenko, A. L.; Pliss, E. M.


    The abnormally high mass-dependent isotope effects in liquid-phase hydrogen (deuterium) atom transfer reactions, which are customarily regarded as quantum effects, are actually the products of two classical effects, namely, kinetic and thermodynamic ones. The former is determined by the rate constants for atom transfer and the latter is caused by nonbonded (or noncovalent) isotope effects in the solvation of protiated and deuterated reacting molecules. This product can mimic the large isotope effects that are usually attributed to tunnelling. In enzymatic reactions, tunnelling is of particular interest; its existence characterizes an enzyme as a rigid molecular machine in which the residence time of reactants on the reaction coordinate exceeds the waiting time for the tunnelling event. The magnitude of isotope effect becomes a characteristic parameter of the internal dynamics of the enzyme catalytic site. The bibliography includes 61 references.

  15. Coupling quantum tunneling with cavity photons. (United States)

    Cristofolini, Peter; Christmann, Gabriel; Tsintzos, Simeon I; Deligeorgis, George; Konstantinidis, George; Hatzopoulos, Zacharias; Savvidis, Pavlos G; Baumberg, Jeremy J


    Tunneling of electrons through a potential barrier is fundamental to chemical reactions, electronic transport in semiconductors and superconductors, magnetism, and devices such as terahertz oscillators. Whereas tunneling is typically controlled by electric fields, a completely different approach is to bind electrons into bosonic quasiparticles with a photonic component. Quasiparticles made of such light-matter microcavity polaritons have recently been demonstrated to Bose-condense into superfluids, whereas spatially separated Coulomb-bound electrons and holes possess strong dipole interactions. We use tunneling polaritons to connect these two realms, producing bosonic quasiparticles with static dipole moments. Our resulting three-state system yields dark polaritons analogous to those in atomic systems or optical waveguides, thereby offering new possibilities for electromagnetically induced transparency, room-temperature condensation, and adiabatic photon-to-electron transfer.

  16. Phonon tunneling through a double barrier system

    Energy Technology Data Exchange (ETDEWEB)

    Villegas, Diosdado [Departamento de Física, Universidad Central “Marta Abreu” de Las Villas, CP 54830, Santa Clara, Villa Clara (Cuba); Instituto de Física, Universidad Autónoma de Puebla, 18 Sur y San Claudio, Edif. 110A, Ciudad Universitaria, 72570 Puebla (Mexico); León-Pérez, Fernando de [Centro Universitario de la Defensa de Zaragoza, Ctra. de Huesca s/n, E-50090 Zaragoza (Spain); Pérez-Álvarez, R. [Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, CP 62209 Cuernavaca (Mexico); Arriaga, J., E-mail: [Instituto de Física, Universidad Autónoma de Puebla, 18 Sur y San Claudio, Edif. 110A, Ciudad Universitaria, 72570 Puebla (Mexico)


    The tunneling of optical and acoustic phonons at normal incidence on a double-barrier is studied in this paper. Transmission coefficients and resonance conditions are derived theoretically under the assumption that the long-wavelength approximation is valid. It is shown that the behavior of the transmission coefficients for the symmetric double barrier has a Lorentzian form close to resonant frequencies and that Breit–Wigner's formula have a general validity in one-dimensional phonon tunneling. Authors also study the so-called generalized Hartman effect in the tunneling of long-wavelength phonons and show that this effect is a numerical artifact resulting from taking the opaque limit before exploring the variation with a finite barrier width. This study could be useful for the design of acoustic devices.

  17. Quantum tunneling from paths in complex time

    CERN Document Server

    Bramberger, Sebastian F; Lehners, Jean-Luc


    We study quantum mechanical tunneling using complex solutions of the classical field equations. Simple visualization techniques allow us to unify and generalize previous treatments, and straightforwardly show the connection to the standard approach using Euclidean instanton solutions. We demonstrate that the negative modes of solutions along various contours in the complex time plane reveal which paths contribute to tunneling and which do not, and we provide a criterion for identifying the negative modes. Central to our approach is the solution of the background and perturbation equations not only along a single path, but over an extended region of the complex time plane. Our approach allows for a fully continuous and coherent treatment of classical evolution interspersed by quantum tunneling events, and is applicable in situations where singularities are present and also where Euclidean solutions might not exist.

  18. Electrodiagnostic approach to carpal tunnel syndrome

    Directory of Open Access Journals (Sweden)

    Cherian Ajith


    Full Text Available An algorithm for an electrophysiological approach to carpal tunnel syndrome is proposed. This technical note takes into account the standard tests, comparison tests and needle electromyography. If the standard tests are negative, a comparison study can be done to identify cases of minimal or very mild carpal tunnel syndrome (CTS. If comparison studies are negative, CTS can be ruled out and a search for other causes have to be made. If the standard tests are positive, carpal tunnel syndrome can be divided into extreme, severe,moderate and mild cases. Motor comparison study is useful in extreme CTS cases. Needle electromyography is a must in all cases where the standard tests are positive. This streamlined approach allows accurate diagnosis with minimum essential tests.

  19. Quantum tunneling from high dimensional G\\"odel black hole

    CERN Document Server

    Li, Hui-Ling; Zu, Xiao-Tao


    Considering quantum gravity effect, we investigate the quantum tunneling from high dimensional Kerr-G\\"odel black hole using generalized Dirac equation. As a result, revised tunneling probability is obtained, and the corrected Hawking temperature is also presented.

  20. Carpal tunnel syndrome after 22 years of Colle's fracture

    Directory of Open Access Journals (Sweden)

    Goyal V


    Full Text Available Delayed carpal tunnel syndrome is rare. We describe the electrophysiological findings in a patient with Colle's fracture, who developed carpal tunnel syndrome 22 years after a wrist injury.

  1. Carpal tunnel syndrome: an unusual presentation of brachial hypertrophy.


    Shenoy, K. T.; Saha, P. K.; Ravindran, M


    A patient with carpal tunnel syndrome in association with congenital hypertrophy of right upper limb is described. The median nerve also showed hypertrophy. The symptoms were relieved by decompression of the carpal tunnel.

  2. Cooperative Bacterial Foraging Optimization

    Directory of Open Access Journals (Sweden)

    Hanning Chen


    Full Text Available Bacterial Foraging Optimization (BFO is a novel optimization algorithm based on the social foraging behavior of E. coli bacteria. This paper presents a variation on the original BFO algorithm, namely, the Cooperative Bacterial Foraging Optimization (CBFO, which significantly improve the original BFO in solving complex optimization problems. This significant improvement is achieved by applying two cooperative approaches to the original BFO, namely, the serial heterogeneous cooperation on the implicit space decomposition level and the serial heterogeneous cooperation on the hybrid space decomposition level. The experiments compare the performance of two CBFO variants with the original BFO, the standard PSO and a real-coded GA on four widely used benchmark functions. The new method shows a marked improvement in performance over the original BFO and appears to be comparable with the PSO and GA.

  3. Bacterial Colony Optimization

    Directory of Open Access Journals (Sweden)

    Ben Niu


    Full Text Available This paper investigates the behaviors at different developmental stages in Escherichia coli (E. coli lifecycle and developing a new biologically inspired optimization algorithm named bacterial colony optimization (BCO. BCO is based on a lifecycle model that simulates some typical behaviors of E. coli bacteria during their whole lifecycle, including chemotaxis, communication, elimination, reproduction, and migration. A newly created chemotaxis strategy combined with communication mechanism is developed to simplify the bacterial optimization, which is spread over the whole optimization process. However, the other behaviors such as elimination, reproduction, and migration are implemented only when the given conditions are satisfied. Two types of interactive communication schemas: individuals exchange schema and group exchange schema are designed to improve the optimization efficiency. In the simulation studies, a set of 12 benchmark functions belonging to three classes (unimodal, multimodal, and rotated problems are performed, and the performances of the proposed algorithms are compared with five recent evolutionary algorithms to demonstrate the superiority of BCO.

  4. Bacterial assays for recombinagens. (United States)

    Hoffmann, G R


    Two principal strategies have been used for studying recombinagenic effects of chemicals and radiation in bacteria: (1) measurement of homologous recombination involving defined alleles in a partially diploid strain, and (2) measurement of the formation and loss of genetic duplications in the bacterial chromosome. In the former category, most methods involve one allele in the bacterial chromosome and another in a plasmid, but it is also possible to detect recombination between two chromosomal alleles or between two extrachromosomal alleles. This review summarizes methods that use each of these approaches for detecting recombination and tabulates data on agents that have been found to be recombinagenic in bacteria. The assays are discussed with respect to their effectiveness in testing for recombinagens and their potential for elucidating mechanisms underlying recombinagenic effects.

  5. Resonant tunneling in graphene pseudomagnetic quantum dots. (United States)

    Qi, Zenan; Bahamon, D A; Pereira, Vitor M; Park, Harold S; Campbell, D K; Neto, A H Castro


    Realistic relaxed configurations of triaxially strained graphene quantum dots are obtained from unbiased atomistic mechanical simulations. The local electronic structure and quantum transport characteristics of y-junctions based on such dots are studied, revealing that the quasi-uniform pseudomagnetic field induced by strain restricts transport to Landau level- and edge state-assisted resonant tunneling. Valley degeneracy is broken in the presence of an external field, allowing the selective filtering of the valley and chirality of the states assisting in the resonant tunneling. Asymmetric strain conditions can be explored to select the exit channel of the y-junction.

  6. A study for the KAERI research tunnel

    Energy Technology Data Exchange (ETDEWEB)

    Kim, J.; Hwang, Y. S.; Park, H. S.; Park, S. K.; Park, B. Y.; Bang, K. S.; Kuh, J. H.; Kang, K. H


    Major goal of the R and D on the KAERI Research Tunnel in 1997 are 1) concept development of the KAERI research tunnel and its major units 2) computer simulation of facilities 3) study on thermo-hydro mechanical coupling in the vicinity of a waste repository 4) effect of excavated distrubed zone. In addition supplementary site investigation to understand the distribution of stresses in the site was done along with long term monitoring of the water table. (author). 44 refs., 16 tabs., 36 figs

  7. Dephasing of quantum tunnelling in molecular nanomagnets

    Institute of Scientific and Technical Information of China (English)

    Zhang Shu-Qun; Chen Zhi-De


    Dephasing mechanism of quantum tunnelling in molecular magnets has been studied by means of the spin-coherentstate path integral in a mean field approximation. It is found that the fluctuating uncompensated transverse field from the dipolar-dipolar interaction between molecular magnets contributes a random phase to the quantum interference phase. The resulting transition rate is determined by the average tunnel splitting over the random phase. Such a dephasing process leads to the suppression of quenching due to the quantum phase interference, and to the steps due to odd resonances in hysteresis loop survived, which is in good agreement with experimental observations in molecular nanomagnets Fee and Mn12.

  8. Prevalence of carpal tunnel syndrome in motorcyclists. (United States)

    Manes, Harvey R


    Carpal tunnel syndrome is prevalent in patients who have a repetitive motion, vibration, or pressure exerted on the wrist joint for an extended period of time. The prevalence of this condition in the general population is approximately 5%. Motorcyclists subject themselves to high levels of vibration from the road and use their wrists to control the motorcycle's brakes, gas intake, and gears via the handlebars. Under these conditions, the author hypothesized that an increased prevalence of carpal tunnel syndrome would be observed in this population.

  9. Treatment of repetitive use carpal tunnel syndrome (United States)

    Smith, Chadwick F.; Vangsness, C. Thomas; Anderson, Thomas; Good, Wayne


    In 1990, a randomized, double-blind study was initiated to evaluate the use of an eight-point conservative treatment program in carpal tunnel syndrome. A total of 160 patients were delineated with symptoms of carpal tunnel syndrome. These patients were then divided into two groups. Both groups were subjected to an ergonomically correct eight-point work modification program. A counterfeit low level laser therapy unit was utilized in Group A, while an actual low level laser therapy unit was utilized in Group B. The difference between Groups A and B was statistically significant in terms of return to work, conduction study improvement, and certain range of motion and strength studies.

  10. Wind Tunnel Measurements at LM Wind Power

    DEFF Research Database (Denmark)

    Bertagnolio, Franck


    This section presents the results obtained during the experimental campaign that was conducted in the wind tunnel at LM Wind Power in Lunderskov from August 16th to 26th, 2010. The goal of this study is to validate the so-called TNO trailing edge noise model through measurements of the boundary...... layer turbulence characteristics and the far-field noise generated by the acoustic scattering of the turbulent boundary layer vorticies as they convect past the trailing edge. This campaign was conducted with a NACA0015 airfoil section that was placed in the wind tunnel section. It is equipped with high...

  11. Gauge-independence of tunneling rates

    CERN Document Server

    Plascencia, Alexis D


    It is shown that tunneling rates can be defined in terms of a false-vacuum effective action whose reality and convexity properties differ from those of the corresponding groundstate functional. The tunneling rate is directly related to the false-vacuum effective action evaluated at an extremal "quantum bounce". The Nielsen identities of the false-vacuum functional ensure that the rate remains independent of the choice of gauge-fixing. Our results are nonperturbative and clarify issues related with convexity and radiative corrections.

  12. Traceable long range scanning tunneling microscopy

    Institute of Scientific and Technical Information of China (English)


    Dimensionally correct and directly traceable measurement is not feasible with conventional scanning tunneling microscopy (STMs) due to severe hysteresis and non-linearity of the commonly applied piezo tube scanners and the very short range.By integrating a custom made probing system based on tunneling current measurement into a commercially available and laser-interferometrically position controlled positioning system, an STM with a principal measuring range of 25 mm×25 mm×5 mm and traceable position measurement has been set-up and tested.

  13. Polaronic Tunnelling in Organic Triblock Copolymers

    Institute of Scientific and Technical Information of China (English)

    LIU De-Sheng; ZHANG Da-Cheng; XIE Shi-Jie; MEI Liang-Mo


    @@ Polaron tunnelling is studied in xPA/nPPP/xPA (PA for polyacetylene and PPP poly (p-phenylene)) triblock copolymer, which has a well-barrier-well structure. An extended tight-binding Hamiltonian including external electric field is adopted. Without electric field, the injected electrons would not extend over the whole copolymer chain but instead be confined in the segments of PA. This is different from the behaviour of the traditional semiconductors. It is found that the polaron can transfer to the potential barrier-PPP segment when the applied electric field reaches a certain value. The critical polaron tunnelling electric fields depend upon the lengths of PPP segments.

  14. Vibration measurement and modal analysis for tunneller

    Institute of Scientific and Technical Information of China (English)

    HUANG Min; WU Miao; WEI Ren-zhi


    In this paper, the method of vibration measurement and modal analysis for AM~50 Tunneller machine is presented. When the machine was used for cutting man-made coal bed and real coal bed, the vibration of the machine was measured and the results of signal analysis show that the vibration characteristics under the two kinds of working situations are similar. The modal model of the machine is established, and then, the intrinsic vibration characteristics of AM50 tunneller are investigated by means of the method of experimental modal analysis. The vibration response simulation under a set of loading spectra measured is carried out by force response simulation software.

  15. Fire Resistant Panels for the Tunnel Linings

    Directory of Open Access Journals (Sweden)

    Gravit Marina


    Full Text Available Presents the results of studies of innovative materials in the field of experimental and theoretical research fire resistance fireproof panels Pyro-Safe Aestuver T. Owing to the assembly simplicity, materials cheapness, high ecological standard, recycling, reuse potential, are benefit. Research work is running to improve the knowledge about fireproof panels Pyro-Safe Aestuver T for tunnel lining, its basic performance, its long term behavior and in particular also its fire proof for example when used for the lining of road tunnels.

  16. Bacterial transformation of terpenoids (United States)

    Grishko, V. V.; Nogovitsina, Y. M.; Ivshina, I. B.


    Data on the bacterial transformation of terpenoids published in the literature in the past decade are analyzed. Possible pathways for chemo-, regio- and stereoselective modifications of terpenoids are discussed. Considerable attention is given to new technological approaches to the synthesis of terpenoid derivatives suitable for the use in the perfume and food industry and promising as drugs and chiral intermediates for fine organic synthesis. The bibliography includes 246 references.

  17. Spontaneous bacterial peritonitis


    Al Amri Saleh


    Spontaneous bacterial peritonitis (SBP) is an infection of the ascitic fluid without obvious intra-abdominal source of sepsis; usually complicates advanced liver disease. The pathogenesis of the disease is multifactorial: low ascitic protein-content, which reflects defi-cient ascitic fluid complement and hence, reduced opsonic activity is thought to be the most important pathogenic factor. Frequent and prolonged bacteremia has been considered as another pertinent cause of SBP. This disease is...

  18. Modelling bacterial speciation



    A central problem in understanding bacterial speciation is how clusters of closely related strains emerge and persist in the face of recombination. We use a neutral Fisher–Wright model in which genotypes, defined by the alleles at 140 house-keeping loci, change in each generation by mutation or recombination, and examine conditions in which an initially uniform population gives rise to resolved clusters. Where recombination occurs at equal frequency between all members of the population, we o...

  19. Bias dependent inversion of tunneling magnetoresistance in Fe/GaAs/Fe tunnel junctions


    Moser, J.; Zenger, M.; Gerl, C.; Schuh, D.; Meier, R.; Chen, P.; Bayreuther, G.; Wegscheider, W.; Weiss, D; Lai, C. -H.; Huang, R. -T.; Kosuth, M.; Ebert, H.


    We investigated spin dependent transport through Fe/GaAs/Fe tunnel junctions. The tunneling magnetoresistance effect (TMR) was probed for different types of Fe/GaAs interfaces. For interfaces cleaned by hydrogen plasma the TMR effect is increased and observable at room temperature. If an epitaxial Fe/GaAs(001) interface is involved, the tunnel junction exhibits a bias dependent inversion of the TMR effect. This is a first experimental signature for band structure effects at a Fe/GaAs interfac...

  20. Doka formwork system SL-1 for tunneling. Example: Šentvid tunnel


    Pogačar, Boris


    The present work focuses on formwork system SL-1, which was developed by the company Doka - die Schalungstechniker for forming of tunnels and special structures. Formwork in general is presented, along with the methods of tunneling, which are in use today. Doka formwork system SL-1 and its typical parts are described in detail. The use of this formwork system is represented on the example of a cavern in Šentvid tunnel. Discussed are two possible shapes of the cavern: the form of three resembl...

  1. Adaptive Bacterial Foraging Optimization

    Directory of Open Access Journals (Sweden)

    Hanning Chen


    Full Text Available Bacterial Foraging Optimization (BFO is a recently developed nature-inspired optimization algorithm, which is based on the foraging behavior of E. coli bacteria. Up to now, BFO has been applied successfully to some engineering problems due to its simplicity and ease of implementation. However, BFO possesses a poor convergence behavior over complex optimization problems as compared to other nature-inspired optimization techniques. This paper first analyzes how the run-length unit parameter of BFO controls the exploration of the whole search space and the exploitation of the promising areas. Then it presents a variation on the original BFO, called the adaptive bacterial foraging optimization (ABFO, employing the adaptive foraging strategies to improve the performance of the original BFO. This improvement is achieved by enabling the bacterial foraging algorithm to adjust the run-length unit parameter dynamically during algorithm execution in order to balance the exploration/exploitation tradeoff. The experiments compare the performance of two versions of ABFO with the original BFO, the standard particle swarm optimization (PSO and a real-coded genetic algorithm (GA on four widely-used benchmark functions. The proposed ABFO shows a marked improvement in performance over the original BFO and appears to be comparable with the PSO and GA.

  2. Neglected bacterial zoonoses. (United States)

    Chikeka, I; Dumler, J S


    Bacterial zoonoses comprise a group of diseases in humans or animals acquired by direct contact with or by oral consumption of contaminated animal materials, or via arthropod vectors. Among neglected infections, bacterial zoonoses are among the most neglected given emerging data on incidence and prevalence as causes of acute febrile illness, even in areas where recognized neglected tropical diseases occur frequently. Although many other bacterial infections could also be considered in this neglected category, five distinct infections stand out because they are globally distributed, are acute febrile diseases, have high rates of morbidity and case fatality, and are reported as commonly as malaria, typhoid or dengue virus infections in carefully designed studies in which broad-spectrum diagnoses are actively sought. This review will focus attention on leptospirosis, relapsing fever borreliosis and rickettsioses, including scrub typhus, murine typhus and spotted fever group rickettsiosis. Of greatest interest is the lack of distinguishing clinical features among these infections when in humans, which confounds diagnosis where laboratory confirmation is lacking, and in regions where clinical diagnosis is often attributed to one of several perceived more common threats. As diseases such as malaria come under improved control, the real impact of these common and under-recognized infections will become evident, as will the requirement for the strategies and allocation of resources for their control.

  3. Large tunneling magnetoresistance in octahedral Fe3O4 nanoparticles


    Arijit Mitra; Barun Barick; Jeotikanta Mohapatra; Sharma, H.; Meena, S. S.; ASLAM, M.


    We have observed large tunneling Magnetoresistance (TMR) in amine functionalized octahedral nanoparticle assemblies. Amine monolayer on the surface of nanoparticles acts as an insulating barrier between the semimetal Fe3O4 nanoparticles and provides multiple tunnel junctions where inter-granular tunneling is plausible. The tunneling magnetoresistance recorded at room temperature is 38% which increases to 69% at 180 K. When the temperature drops below 150 K, coulomb staircase is observed in th...

  4. Dynamic Instability of Tunnel in Blocky Rock Mass

    Institute of Scientific and Technical Information of China (English)

    QI Chengzhi; CHEN Canshou; QIAN Qihu; LUO Jian


    The displacements and geometry of the rock blocks and the properties of the rock structure play an important role in the stability of tunnels.Based on the key block model, the dynamic instability analysis of underground tunnel subjected to intensive short-time compressional wave was conducted.The instability of the tunnel caused by the spallation and the inertial effect was distinguished.And the influence of the roof contour curvature of tunnel was also determined.

  5. Fabrication of magnetic tunnel junctions with epitaxial and textured ferromagnetic layers (United States)

    Chang, Y. Austin; Yang, Jianhua Joshua


    This invention relates to magnetic tunnel junctions and methods for making the magnetic tunnel junctions. The magnetic tunnel junctions include a tunnel barrier oxide layer sandwiched between two ferromagnetic layers both of which are epitaxial or textured with respect to the underlying substrate upon which the magnetic tunnel junctions are grown. The magnetic tunnel junctions provide improved magnetic properties, sharper interfaces and few defects.

  6. Flow Cytometric Assessment of Bacterial Abundance in Soils, Sediments and Sludge. (United States)

    Frossard, Aline; Hammes, Frederik; Gessner, Mark O


    Bacterial abundance is a fundamental measure in microbiology, but its assessment is often tedious, especially for soil, and sediment samples. To overcome this limitation, we adopted a time-efficient flow-cytometric (FCM) counting method involving cell detachment and separation from matrix particles by centrifugation in tubes receiving sample suspensions and Histodenz(®) solution. We used this approach to assess bacterial abundances in diverse soils (natural and agricultural), sediments (streams and lakes) and sludge from sand-filters in a drinking water treatment plant and compared the results to bacterial abundances determined by two established methods, epifluorescence microscopy (EM) and adenosine triphosphate (ATP) quantification. Cell abundances determined by FCM and EM correlated fairly well, although absolute cell abundances were generally lower when determined by FCM. FCM also showed significant relations with cell counts converted from ATP concentrations, although estimates derived from ATP determinations were typically higher, indicating the presence of ATP sources other than bacteria. Soil and sediment organic matter (OM) content influenced the goodness of fit between counts obtained with EM and FCM. In particular, bacterial abundance determined by FCM in samples containing less than 10% OM, such as stream sediment, was particularly well correlated with the cell counts assessed by EM. Overall, these results suggest that FCM following cell detachment and purification is a useful approach to increase sample throughput for determining bacterial abundances in soils, sediments and sludge. However, notable scatter and only partial concordance among the FCM and reference methods suggests that protocols require further improvement for assessments requiring high precision, especially when OM contents in samples are high.

  7. Consideration of rotational tunneling in rare gas cluster ions (United States)

    Albertoni, C. R.; Ferguson, A. W. Castleman E. E., Jr.


    Tunneling transmission coefficients through rotational barriers are investigated as a function of argon cluster ion size using the WKB approximation. The results indicate that tunneling coefficients corresponding to transmissions of a few percent, extend over time windows of ten orders of magnitude. Hence, a very broad distribution of metastable lifetimes is expected for decay of cluster ions by tunneling through rotational barriers.

  8. Alendronate reduced peri-tunnel bone loss and enhanced tendon graft to bone tunnel healing in anterior cruciate ligament reconstruction

    Directory of Open Access Journals (Sweden)

    PPY Lui


    Full Text Available Peri-tunnel bone loss after anterior cruciate ligament (ACL reconstruction is commonly observed, both clinically and experimentally. We aimed to study the effect and mechanisms of different doses of alendronate in the reduction of peri-tunnel bone loss and promotion of graft-bone tunnel healing in ACL reconstruction. Eighty-four ACL-reconstructed rats were divided into 4 groups. Alendronate at different dosages, or saline, were injected subcutaneously weekly, for 2 or 6 weeks post-reconstruction, for vivaCT (computed tomography imaging, biomechanical tests, histology and immunohistochemistry. Alendronate significantly increased bone mass and density of tissue inside bone tunnels except at the epiphyseal region of tibial tunnel. The femoral tunnel diameter decreased significantly in the mid-dose and high-dose alendronate groups compared to that in the saline group at week 6. Alendronate significantly increased the peri-tunnel bone mass and density along all tunnel regions at week 6. Better graft-bone tunnel integration and intra-tunnel graft integrity were observed in the alendronate groups. The ultimate load was significantly higher in the mid-dose and high-dose alendronate groups at week 2, but not at week 6. There was a reduction in matrix metalloprotein (MMP1, MMP13 and CD68-positive cells at the peri-tunnel region and graft-bone interface in the alendronate-treated group compared to the saline group. Alendronate reduced peri-tunnel bone resorption, increased mineralised tissue inside bone tunnel as well as histologically and biomechanically promoted graft-bone tunnel healing, probably by reducing the expression of MMP1, MMP13 and CD68-positive cells. Alendronate might be used for reducing peri-tunnel bone loss and promoting graft-bone tunnel healing at early stage post-ACL reconstruction.

  9. Anomalous Tunnel Magnetoresistance and Spin Transfer Torque in Magnetic Tunnel Junctions with Embedded Nanoparticles (United States)

    Useinov, Arthur; Ye, Lin-Xiu; Useinov, Niazbeck; Wu, Te-Ho; Lai, Chih-Huang


    The tunnel magnetoresistance (TMR) in the magnetic tunnel junction (MTJ) with embedded nanoparticles (NPs) was calculated in range of the quantum-ballistic model. The simulation was performed for electron tunneling through the insulating layer with embedded magnetic and non-magnetic NPs within the approach of the double barrier subsystem connected in parallel to the single barrier one. This model can be applied for both MTJs with in-plane magnetization and perpendicular one. We also calculated the in-plane component of the spin transfer torque (STT) versus the applied voltage in MTJs with magnetic NPs and determined that its value can be much larger than in single barrier system (SBS) for the same tunneling thickness. The reported simulation reproduces experimental data of the TMR suppression and peak-like TMR anomalies at low voltages available in leterature.

  10. Asymmetric voltage behavior of the tunnel magnetoresistance in double barrier magnetic tunnel junctions

    KAUST Repository

    Useinov, Arthur


    In this paper, we study the value of the tunnel magnetoresistance (TMR) as a function of the applied voltage in double barrier magnetic tunnel junctions (DMTJs) with the left and right ferromagnetic (FM) layers being pinned and numerically estimate the possible difference of the TMR curves for negative and positive voltages in the homojunctions (equal barriers and electrodes). DMTJs are modeled as two single barrier junctions connected in series with consecutive tunneling (CST). We investigated the asymmetric voltage behavior of the TMR for the CST in the range of a general theoretical model. Significant asymmetries of the experimental curves, which arise due to different annealing regimes, are mostly explained by different heights of the tunnel barriers and asymmetries of spin polarizations in magnetic layers. © (2012) Trans Tech Publications.

  11. Resonant tunnel magnetoresistance in double-barrier planar magnetic tunnel junctions

    KAUST Repository

    Useinov, A. N.


    We present a theoretical approach to calculate the spin-dependent current and tunnel magnetoresistance (TMR) in a double-barrier magnetic tunnel junction (DMTJ), in which the magnetization of the middle ferromagnetic metal layer can be aligned parallel or antiparallel in relation to the fixed magnetizations of the left and right ferromagnetic electrodes. The electron transport through the DMTJ is considered as a three-dimensional problem, taking into account all transmitting electron trajectories as well as the spin-dependent momentum conservation law. The dependence of the transmission coefficient and spin-polarized currents on the applied voltage is derived as an exact solution to the quantum-mechanical problem for the spin-polarized transport. In the range of the developed physical model, the resonant tunneling, nonresonant tunneling, and enhanced spin filtering can be explained; the simulation results are in good agreement with experimental data.

  12. Colloquium on mine shaft, roadway and tunnel. Proceedings; Kolloquium Schacht, Strecke und Tunnel 2005. Tagungsband

    Energy Technology Data Exchange (ETDEWEB)



    The conference intended to outline the economic mechanisms of the coal sector and to show the importance of mining and tunneling and the competence in problem solving acquired in this area. Efficient and sustainable use of domestic reserves may help to ensure long-term supply and keep prices stable. Most of the required infrastructure will be located underground in this highly populated country. Technologies of tunneling and horizontal drilling are described and illustrated by the example of a new Cologne underground railway project. Mining and tunnelling have much in common, e.g. drilling and shotfiring, overburden removal, rock strength, supports, protection against liquid and gas leakage, ventilation and air conditioning. Projects like the new Austrian high-speed railway track or the 'Loetschbergbasistunnel' project in Switzerland are examples of tunneling project which are clearly comparable with the construction of a new coal mine.

  13. Planar Tunneling Spectroscopy of Graphene Nanodevices (United States)

    Wang, Joel I.-Jan; Bretheau, Landry; Pisoni, Riccardo; Watanabe, Kenji; Taniguchi, Takashi; Jarillo-Herrero, Pablo

    2-D Van-der-Waals mesoscopic physics have seen a rapid development in the last 10 years, with new materials each year added to the toolbox. Stacking them like Lego enables the combination of their individual electronic properties. In particular, hexagonal boron nitride, which is an insulator, gives the possibility to perform planar (2-D to 2-D) tunneling spectroscopy within this type of heterostructures. Unlike standard transport measurements, tunneling spectroscopy enables to probe the electronic properties in the energy domain. Moreover, since planar tunneling probes a large area of the system, global quantum features such as quantum Hall effect, superconducting proximity effect or quantum confinement can be investigated. In this talk, we will present implementation of heterostructures consisting of graphene, hexagonal boron nitride, and graphite, fabricated for planar tunneling spectroscopy. In order to reveal the intrinsic properties of materials, the fabrication scheme aims at preserving the pristine nature of the 2-DEGS as well as minimizing the doping introduced by external probes. As a demonstration, measurements of these devices in normal states, high magnetic field environment, and induced superconducting state will be presented.

  14. A Student-Built Scanning Tunneling Microscope (United States)

    Ekkens, Tom


    Many introductory and nanotechnology textbooks discuss the operation of various microscopes including atomic force (AFM), scanning tunneling (STM), and scanning electron microscopes (SEM). In a nanotechnology laboratory class, students frequently utilize microscopes to obtain data without a thought about the detailed operation of the tool itself.…

  15. Integrated tunneling sensor for nanoelectromechanical systems

    DEFF Research Database (Denmark)

    Sadewasser, S.; Abadal, G.; Barniol, N.


    Transducers based on quantum mechanical tunneling provide an extremely sensitive sensor principle, especially for nanoelectromechanical systems. For proper operation a gap between the electrodes of below 1 nm is essential, requiring the use of structures with a mobile electrode. At such small...

  16. Prismatic Blade Measuring on a Wind Tunnel

    Directory of Open Access Journals (Sweden)

    Stech J.


    Full Text Available The results from measurement on the straight blade cascade are presented in the paper. The cascade is placed at the outlet of wind tunnel in ŠKODA POWER experimental base. The results in the form of velocity and loss fields behind blade cascade as well as the distribution of the loss coefficient in selected cross-section are evaluated.

  17. Resonant tunneling in a pulsed phonon field

    DEFF Research Database (Denmark)

    Kral, P.; Jauho, Antti-Pekka


    We theoretically investigate resonant tunneling through a single level assisted by short LO phonon pulses. The analysis is based on the recently developed nonequilibrium linked-cluster expansion [P. Kral, Phys. Rev. B 56, 7293 (1997)], extended in this work to transient situations, The nonequilib...

  18. The overshoot problem in inflation after tunneling

    Energy Technology Data Exchange (ETDEWEB)

    Dutta, Koushik; Vaudrevange, Pascal M.; Westphal, Alexander


    We show the absence of the usual parametrically large overshoot problem of small-field inflation if initiated by a Coleman-De Luccia (CDL) tunneling transition from an earlier vacuum in the limit of small inflationary scale compared to the tunneling scale. For low-power monomial exit potentials V({phi}){proportional_to} {phi}{sup n}; n<4, we derive an expression for the amount of overshoot. This is bounded from above by the width of the steep barrier traversed after emerging from tunneling and before reaching a slow-roll region of the potential. For n{>=}4 we show that overshooting is entirely absent. We extend this result through binomials to a general potential written as a series expansion, and to the case of arbitrary finite initial speed of the inflaton. This places the phase space of initial conditions for small-field and large-field inflation on the same footing in a landscape of string theory vacua populated via CDL tunneling. (orig.)

  19. Hemodialysis Tunneled Catheter-Related Infections (United States)

    Miller, Lisa M.; Clark, Edward; Dipchand, Christine; Hiremath, Swapnil; Kappel, Joanne; Kiaii, Mercedeh; Lok, Charmaine; Luscombe, Rick; Moist, Louise; Oliver, Matthew; MacRae, Jennifer


    Catheter-related bloodstream infections, exit-site infections, and tunnel infections are common complications related to hemodialysis central venous catheter use. The various definitions of catheter-related infections are reviewed, and various preventive strategies are discussed. Treatment options, for both empiric and definitive infections, including antibiotic locks and systemic antibiotics, are reviewed.

  20. Single electron tunneling based arithmetic computation

    NARCIS (Netherlands)

    Lageweg, C.R.


    In this dissertation we investigate the implementation of computer arithmetic operations with Single Electron Tunneling (SET) technology based circuits. In our research we focus on the effective utilization of the SET technologys specific characteristic, i.e., the ability to control the transport of

  1. Theoretical Analysis of Reinforcement Tunnel Lining Corrosion

    Directory of Open Access Journals (Sweden)

    ZhiQiang Zhangand


    Full Text Available The main cause of ageing damage in reinforced concrete structures is reinforcement corrosion. Damage can be detected visually as coincident cracks along the reinforcement bar, which are significant of both reduction of the re-bar, cross-section and loss of bond strength for reinforced concrete. The reinforced concrete is one of the most widely used engineering materials as final lining of tunnels. The corrosion is common durability problems that have significant effect on the tunnel performance. This study intends to analysis reinforcement concrete corrosion at the tunnel lining by applying temperature expansion theory on steel through numerical simulation process, with expansive force effect. The thickness of concrete cover and the diameter of steel bar have an impact on the stress for reinforcement concrete during propagation of corrosion process. The corrosion cracks appear at the corner of a tunnel lining then in invert and vault because the maximum stress will be in the corner then in invert and vault. The internal force in the concrete lining changes differently when the corrosion rate change.


    Institute of Scientific and Technical Information of China (English)

    徐磊; 顾冬红; 等


    Scanning tunneling microscope(STM) is used to investigate the optical dise.The areas with and without data stampers are all observedcarefully.Three-dimensional images of the disc surface clearly demonstrate the period.depth of the grooves and the shape of data stampers.Some phenomena of STM imaging are also discussed.

  3. Microscopic tunneling theory of long Josephson junctions

    DEFF Research Database (Denmark)

    Grønbech-Jensen, N.; Hattel, Søren A.; Samuelsen, Mogens Rugholm


    We present a numerical scheme for solving a nonlinear partial integro-differential equation with nonlocal time dependence. The equation describes the dynamics in a long Josephson junction modeled by use of the microscopic theory for tunneling between superconductors. We demonstrate...

  4. An Optimization Model of Tunnel Support Parameters

    Directory of Open Access Journals (Sweden)

    Su Lijuan


    Full Text Available An optimization model was developed to obtain the ideal values of the primary support parameters of tunnels, which are wide-ranging in high-speed railway design codes when the surrounding rocks are at the III, IV, and V levels. First, several sets of experiments were designed and simulated using the FLAC3D software under an orthogonal experimental design. Six factors, namely, level of surrounding rock, buried depth of tunnel, lateral pressure coefficient, anchor spacing, anchor length, and shotcrete thickness, were considered. Second, a regression equation was generated by conducting a multiple linear regression analysis following the analysis of the simulation results. Finally, the optimization model of support parameters was obtained by solving the regression equation using the least squares method. In practical projects, the optimized values of support parameters could be obtained by integrating known parameters into the proposed model. In this work, the proposed model was verified on the basis of the Liuyang River Tunnel Project. Results show that the optimization model significantly reduces related costs. The proposed model can also be used as a reliable reference for other high-speed railway tunnels.

  5. Carpal Tunnel Syndrome in ARL Libraries. (United States)

    Thornton, Joyce K.


    A survey of 72 member libraries in the Association of Research Libraries revealed the incidence of carpal tunnel syndrome (CTS) and the measures taken to cope with it. Recommends implementing proactive ergonomics programs; soliciting staff input for solutions; providing report guidelines; using external help; stressing preventive measures and…

  6. Employees' Knowledge of Carpal Tunnel Syndrome. (United States)

    Gandy-Goldston, Terrie M.

    A study examined employees' knowledge of the causes of carpal tunnel syndrome (CTS), its prevention, and their legal rights after being diagnosed with CTS. A 24-item questionnaire was administered to a random sample of 30 Chicago-area employees who had been afflicted with CTS. Of those surveyed, 99% considered their CTS injury related to their…

  7. Tunneling spectra of graphene on copper unraveled

    DEFF Research Database (Denmark)

    Zhang, Xin; Stradi, Daniele; Liu, Lei


    mechanisms, etc. The interpretation of the spectra can be complicated, however. Specifically for graphene grown on copper, there have been conflicting reports of tunneling spectra. A clear understanding of the mechanisms behind the variability is desired. In this work, we have revealed that the root cause...

  8. Enhanced Tunnelling Models for Child Universe Formation

    CERN Document Server

    Ansoldi, S; Shilon, I


    Starting from a recently proposed model that allows for an enhanced rate of child universe production under generic conditions, we elaborate on refinements that may allow for non-singular initial configurations. A possibility to treat both, the initial state and the tunnelling beyond the semiclassical level will also be introduced.

  9. Radiologic placement of tunneled central venous catheter

    Energy Technology Data Exchange (ETDEWEB)

    Hahn, Seong Tae; Yang, Po Song; Yang, Dong Hunn; Kim, Ki Tae; Kim, Choon Yul; Shinn, Kyung Sub [The Catholic Univ. College of Medicine, Seoul (Korea, Republic of); Yun, Eun Joo [Korea Veterans Hospital, Seoul (Korea, Republic of)


    To evaluate the efficacy and safety of fluoroscopy-guided, radiologic placement of a tunneled central venous catheter into the superior vena cava (SVC). Thirty five patients underwent tunneled central venous catheter placement to facilitate long-term chemotherapy. They included 33 leukemic patients, one colon cancer patient, and one multiple myeloma patient. After confirming central venous patency with a injection of contrast media via the peripheral cephalic or basilic vein in the wrist joint, the subclavian vein was punctured under fluoroscopic guidance. A 7F double lumen TPN catheter was placed into the SVC through a subcutaneous tunnel in the anterior chest wall. Catheter placements were successful in all patients. The mean procedure time was 17.2minutes, mean fluooscopy time was 1.3minutes, mean number of punctures was 1.4, and mean volume of injhected contrast media was 43.5cc. Only two of all leukemic patients developed mild hematomas at the puncture site, but these soon resolved themselves. None of the patients developed pneumothorax or hemothorax. But late complications included local infection in two patients (6%) and thrombotic occlusion of the catheter in one (3%). The occluded catheter was successfully recanalized with Urokinase infusion. Fluoroscopy-guided, radiologic placement of a tunneled central venous catheter is an easy and safe method, and useful for patients requiring long-term venous access.

  10. Dissipative tunneling of deuterons in Palladium Deuterides (United States)

    Sinha, K. P.; Hagelstein, Peter


    Decisive experimental evidence exists(B.N.Ganguly,Phys.Rev. B14), 3848(1976). for optical phonons being involved in charged particle-phonon interaction in PdD. We consider the tunneling of charged defects (D^+ or D^-) in a two-state system(K.P.Sinha,Mod.Phys.Lett. B1), 805 (1998)^, (P.L.Hagelstein,To be published.)^,(U.Weiss, \\underline Quantum) \\underline Dissipative \\underline Systems (World Scientific, Singapore, 1999).. The two minima of the potential energy are taken to be asymetrical to reflect the real situation occurring in the solid matrix with defects. A pseudospin formalism is used in which the charged particle interacts with conduction electrons as well as optical phonons. The renormalization effects of the tunneling matrix elements due to interaction with electrons and optical phonons are taken into account. The tunneling rate is derived for both coherent and incoherent situations at finite temperatures. The expressions contain factors which show the emission(absorption) of phonons depending on the direction of the tunneling process. Thus the moving deuteron flux stimulates the generation of optical phonons.

  11. Towards high-speed scanning tunneling microscopy

    NARCIS (Netherlands)

    Tabak, Femke Chantal


    In this thesis, two routes towards high-speed scanning tunneling microscopy (STM) are described. The first possibility for high-speed scanning that is discussed is the use of MEMS (Micro-Electro Mechanical Systems) devices as high-speed add-ons in STM microscopes. The functionality of these devices

  12. Bilateral carpal tunnel syndrome in Graves' disease. (United States)

    Manganelli, P; Pavesi, G; Salaffi, F


    Carpal tunnel syndrome (CTS) may be associated with endocrinopathies, such as hypothyroidism and acromegaly. A direct relationship between CTS and hyperthyroidism has recently been suggested. We now report a case in which bilateral CTS developed after treatment of Graves' disease, thus, questioning the possibility of a relationship between these two disease processes.

  13. Cooling of suspended nanostructures with tunnel junctions


    Koppinen, P. J.; Maasilta, I. J.


    We have investigated electronic cooling of suspended nanowires with SINIS tunnel junction coolers. The suspended samples consist of a free standing nanowire suspended by four narrow ($\\sim$ 200 nm) bridges. We have compared two different cooler designs for cooling the suspended nanowire. We demonstrate that cooling of the nanowire is possible with a proper SINIS cooler design.

  14. Tunnel Diode Discriminator with Fixed Dead Time

    DEFF Research Database (Denmark)

    Diamond, J. M.


    A solid state discriminator for the range 0.4 to 10 V is described. Tunnel diodes are used for the discriminator element and in a special fixed dead time circuit. An analysis of temperature stability is presented. The regulated power supplies are described, including a special negative resistance...

  15. 3D numerical design of tunnel hood

    CERN Document Server

    Uystepruyst, David; Monnoyer, François


    This paper relates to the parametric study of tunnel hoods in order to reduce the shape, i.e the temporal gradient, of the pressure wave generated by the entry of a High speed train in tunnel. This is achieved by using an in-house three-dimensional numerical solver which solves the Eulerian equations on a Cartesian and unstructured mesh. The efficiency of the numerical methodology is demonstrated through comparisons with both experimental data and empirical formula. For the tunnel hood design, three parameters, that can influence the wave shape, are considered: the shape, the section and the length of the hood. The numerical results show, (i) that a constant section hood is the most efficient shape when compared to progressive (elliptic or conical) section hoods, (ii) an optimal ratio between hood's section and tunnel section where the temporal gradient of the pressure wave can be reduced by half, (iii) a significant efficiency of the hood's length in the range of 2 to 8 times the length of the train nose. Fi...

  16. Explosion risks and consequences for tunnels

    NARCIS (Netherlands)

    Weerheijm, J.; Berg, A.C. van den


    Tunnel accidents with transports of dangerous goods may lead to explosions. Risk assessment for these accidents is complicated because of the low probability and the unknown, but disastrous effects expected. Especially the lack of knowledge on the strength of the explosion and the consequences for t

  17. Electric field modulation of tunneling anisotropic magnetoresistance in tunnel junctions with antiferromagnetic electrodes (United States)

    Goto, Minori; Nawaoka, Kohei; Miwa, Shinji; Hatanaka, Shohei; Mizuochi, Norikazu; Suzuki, Yoshishige


    We present electric field modulation of tunneling anisotropic magnetoresistance (TAMR) in MnIr|MgO|Ta tunnel junctions. TAMR enables direct observation of the antiferromagnetic spin direction at the MnIr|MgO interface. We found that the shape of magnetoresistance (MR) curve can be modulated by an electric field, which can be explained by electric field modulation of the interfacial magnetic anisotropy at MnIr|MgO.

  18. Pathogenesis and electrodiagnosis of cubital tunnel syndrome

    Institute of Scientific and Technical Information of China (English)

    贾志荣; 石昕; 孙相如


    Background Cubital tunnel syndrome is a well-recognized clinical condition and is the second most common peripheral compression neuropathy. This study was designed to investigate the causes of cubital tunnel syndrome by surgical means and to assess the clinical value of the neurophysiological diagnosis of cubital tunnel syndrome. Methods Twenty-one patients (involving a total of 22 limbs from 16 men and 5 women, aged 22 to 63, with a mean age of 49 years) with clinical symptoms and signs indicating a problem with their ulnar nerve underwent motor conduction velocity examinations at different sites along the ulnar nerve and examinations of sensory conduction velocity in the hand, before undergoing anterior transposition of the ulnar nerve.Results Electromyographic abnormalities were seen in 21 of 22 limbs [motor nerve conduction velocity (MCV) range (15.9-47.5) m/s, mean 32.7 m/s] who underwent motor conduction velocity examinations across the elbow segment of the ulnar nerve. Reduced velocity was observed in 13 of 22 limbs [MCV (15.7-59.6) m/s, mean 40.4 m/s] undergoing MCV tests in the forearms. An absent or abnormal sensory nerve action potential following stimulation was detected in the little finger of 14 of 22 limbs. The factors responsible for ulnar compression based on observations made during surgery were as follows: 15 cases involved compression by arcuate ligaments, muscle tendons, or bone hyperplasia; 2 involved fibrous adhesion; 3 involved compression by the venous plexus or a concurrent thick vein; 2 involved compression by cysts. Conclusions Factors inducing cubital tunnel syndrome include both common factors that have been reported and rare factors, involving the venous plexus, thick veins, and cysts. Tests of motor conduction velocity at different sites along the ulnar nerve should be helpful in diagnosis cubital tunnel syndrome, especially MCV tests indicating decreased velocity across the elbow segment of the ulnar nerve.

  19. Bacterial chromosome segregation. (United States)

    Possoz, Christophe; Junier, Ivan; Espeli, Olivier


    Dividing cells have mechanisms to ensure that their genomes are faithfully segregated into daughter cells. In bacteria, the description of these mechanisms has been considerably improved in the recent years. This review focuses on the different aspects of bacterial chromosome segregation that can be understood thanks to the studies performed with model organisms: Escherichia coli, Bacillus subtilis, Caulobacter crescentus and Vibrio cholerae. We describe the global positionning of the nucleoid in the cell and the specific localization and dynamics of different chromosomal loci, kinetic and biophysic aspects of chromosome segregation are presented. Finally, a presentation of the key proteins involved in the chromosome segregation is made.

  20. Bacterial Degradation of Pesticides

    DEFF Research Database (Denmark)

    Knudsen, Berith Elkær

    This PhD project was carried out as part of the Microbial Remediation of Contaminated Soil and Water Resources (MIRESOWA) project, funded by the Danish Council for Strategic Research (grant number 2104-08-0012). The environment is contaminated with various xenobiotic compounds e.g. pesticides......D student, to construct fungal-bacterial consortia in order to potentially stimulate pesticide degradation thereby increasing the chance of successful bioaugmentation. The results of the project are reported in three article manuscripts, included in this thesis. In manuscript I, the mineralization of 2...

  1. Spontaneous bacterial peritonitis

    Directory of Open Access Journals (Sweden)

    Al Amri Saleh


    Full Text Available Spontaneous bacterial peritonitis (SBP is an infection of the ascitic fluid without obvious intra-abdominal source of sepsis; usually complicates advanced liver disease. The pathogenesis of the disease is multifactorial: low ascitic protein-content, which reflects defi-cient ascitic fluid complement and hence, reduced opsonic activity is thought to be the most important pathogenic factor. Frequent and prolonged bacteremia has been considered as another pertinent cause of SBP. This disease is associated with high mortality and recurrence. Therefore, orompt recognition and institution of therapy and plan of prophylaxis is vital.

  2. Bacterial mitotic machineries

    DEFF Research Database (Denmark)

    Gerdes, Kenn; Møller-Jensen, Jakob; Ebersbach, Gitte;


    Here, we review recent progress that yields fundamental new insight into the molecular mechanisms behind plasmid and chromosome segregation in prokaryotic cells. In particular, we describe how prokaryotic actin homologs form mitotic machineries that segregate DNA before cell division. Thus, the Par......M protein of plasmid R1 forms F actin-like filaments that separate and move plasmid DNA from mid-cell to the cell poles. Evidence from three different laboratories indicate that the morphogenetic MreB protein may be involved in segregation of the bacterial chromosome....

  3. Thermal creation of a spin current by Seebeck spin tunneling (United States)

    Jansen, R.; Le Breton, J. C.; Deac, A. M.; Saito, H.; Yuasa, S.


    The thermoelectric analog of spin-polarized tunneling, namely Seebeck spin tunneling, is a recently discovered phenomenon that arises from the spin-dependent Seebeck coefficient of a magnetic tunnel contact. In a tunnel junction with one ferromagnetic electrode and one non-magnetic electrode, a temperature difference between the two electrodes creates a spin current across the contact. Here, the basic principle and the observation of Seebeck spin tunneling are described. It is shown how it can be used to create a spin accumulation in silicon driven by a heat flow across a magnetic tunnel contact, without a charge tunnel current. The sign of the spin current depends on the direction of the heat flow, whereas its magnitude is anisotropic, i.e., dependent on the absolute orientation of the magnetization of the ferromagnet. The connection between Seebeck spin tunneling and the tunnel magneto-Seebeck effect, observed in metal magnetic tunnel junctions, is also clarified. Seebeck spin tunneling may be used to convert waste heat into useful thermal spin currents that aid or replace electrical spin current, and thereby improve the energy efficiency of spintronic devices and technologies.

  4. Tunneling Time in Ultrafast Science is Real and Probabilistic

    CERN Document Server

    Landsman, Alexandra; Maurer, Jochen; Boge, Robert; Ludwig, André; Heuser, Sebastian; Cirelli, Claudio; Gallmann, Lukas; Keller, Ursula


    We compare the main competing theories of tunneling time against experimental measurements using the attoclock in strong laser field ionization of helium atoms. Refined attoclock measurements reveal a real and not instantaneous tunneling delay time over a large intensity regime, using two different experimental apparatus. Only two of the theoretical predictions are compatible within our experimental error: the Larmor time, and the probability distribution of tunneling times constructed using a Feynman Path Integral (FPI) formulation. The latter better matches the observed qualitative change in tunneling time over a wide intensity range, and predicts a broad tunneling time distribution with a long tail. The implication of such a probability distribution of tunneling times, as opposed to a distinct tunneling time, challenges how valence electron dynamics are currently reconstructed in attosecond science. It means that one must account for a significant uncertainty as to when the hole dynamics begin to evolve.

  5. Videogrammetric Model Deformation Measurement Technique for Wind Tunnel Applications (United States)

    Barrows, Danny A.


    Videogrammetric measurement technique developments at NASA Langley were driven largely by the need to quantify model deformation at the National Transonic Facility (NTF). This paper summarizes recent wind tunnel applications and issues at the NTF and other NASA Langley facilities including the Transonic Dynamics Tunnel, 31-Inch Mach 10 Tunnel, 8-Ft high Temperature Tunnel, and the 20-Ft Vertical Spin Tunnel. In addition, several adaptations of wind tunnel techniques to non-wind tunnel applications are summarized. These applications include wing deformation measurements on vehicles in flight, determining aerodynamic loads based on optical elastic deformation measurements, measurements on ultra-lightweight and inflatable space structures, and the use of an object-to-image plane scaling technique to support NASA s Space Exploration program.

  6. Use of 3D Printing for Custom Wind Tunnel Fabrication (United States)

    Gagorik, Paul; Bates, Zachary; Issakhanian, Emin


    Small-scale wind tunnels for the most part are fairly simple to produce with standard building equipment. However, the intricate bell housing and inlet shape of an Eiffel type wind tunnel, as well as the transition from diffuser to fan in a rectangular tunnel can present design and construction obstacles. With the help of 3D printing, these shapes can be custom designed in CAD models and printed in the lab at very low cost. The undergraduate team at Loyola Marymount University has built a custom benchtop tunnel for gas turbine film cooling experiments. 3D printing is combined with conventional construction methods to build the tunnel. 3D printing is also used to build the custom tunnel floor and interchangeable experimental pieces for various experimental shapes. This simple and low-cost tunnel is a custom solution for specific engineering experiments for gas turbine technology research.

  7. Inelastic scanning tunneling microscopy in conventional and unconventional superconductors

    Energy Technology Data Exchange (ETDEWEB)

    Hlobil, Patrik; Schmalian, Joerg; Wulfhekel, Wulf; Jandke, Jasmin [Karlsruhe Institute of Technologie (Germany)


    Electron tunneling spectroscopy has been used extremely successful in order to verify the microscopic phonon pairing mechanism in conventional BCS superconductors using the Eliashberg theory. Nevertheless, earlier theories and experiments focused mainly on elastic tunneling processes. We present, motivated by recent experiments, a theoretical description of inelastic tunneling in STM in which an electrons tunnels from the tip into a BCS superconductor and coherently excites a phonon during the tunneling process. This additional channel enhances the measured conductivity and we show that if the superconductor is in the normal state, within some limitations, the derivative dσ/dV will be proportional to the Eliashberg function α2F. Additionally, the influence of the inelastic contributions on the tunneling spectrum in the superconducting state will be discussed. Finally, we generalize the theory to other bosonic excitations and focus on the question if inelastic tunneling could be used to unveil the electronic pairing mechanism in the iron pnictides.

  8. Superpoissonian shot noise in organic magnetic tunnel junctions

    Energy Technology Data Exchange (ETDEWEB)

    Cascales, Juan Pedro; Martinez, Isidoro; Aliev, Farkhad G., E-mail: [Dpto. Fisica Materia Condensada C3, Instituto Nicolas Cabrera (INC), Condensed Matter Physics Institute (IFIMAC), Universidad Autonoma de Madrid, Madrid 28049 (Spain); Hong, Jhen-Yong; Lin, Minn-Tsong, E-mail: [Department of Physics, National Taiwan University, Taipei 10617, Taiwan and Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 10617, Taiwan (China); Szczepański, Tomasz; Dugaev, Vitalii K. [Department of Physics, Rzeszów University of Technology, al. Powstańców Warszawy 6, 35-959 Rzeszów (Poland); Barnaś, Józef [Faculty of Physics, Adam Mickiewicz University, ul. Umultowska 85, 61-614 Poznań, Poland and Institute of Molecular Physics, Polish Academy of Sciences, ul. Smoluchowskiego 17, 60-179 Poznań (Poland)


    Organic molecules have recently revolutionized ways to create new spintronic devices. Despite intense studies, the statistics of tunneling electrons through organic barriers remains unclear. Here, we investigate conductance and shot noise in magnetic tunnel junctions with 3,4,9,10-perylene-teracarboxylic dianhydride (PTCDA) barriers a few nm thick. For junctions in the electron tunneling regime, with magnetoresistance ratios between 10% and 40%, we observe superpoissonian shot noise. The Fano factor exceeds in 1.5–2 times the maximum values reported for magnetic tunnel junctions with inorganic barriers, indicating spin dependent bunching in tunneling. We explain our main findings in terms of a model which includes tunneling through a two level (or multilevel) system, originated from interfacial bonds of the PTCDA molecules. Our results suggest that interfaces play an important role in the control of shot noise when electrons tunnel through organic barriers.

  9. Further observations on the utilization of adenosine triphosphate in rat mast cells during histamine release induced by the ionophore A23187

    DEFF Research Database (Denmark)

    Johansen, Torben


    1 The relation between A23187-induced histamine release and the energy metabolism of the rat mast cells has been studied. 2 Ethacrynic acid was used as an inhibitor of calcium-induced histamine release from mast cells primed with the ionophore A23187, and to study calcium-induced changes...... in the adenosine triphosphate (ATP) content and the rate of lactate production of A23187-primed mast cells. 3 Ethacrynic acid by itself decreased the rate of glycolytic ATP production. 4 By measurement of the ATP content and the lactate production of mast cells with or without secretory activity, the increased...... demand of energy for exocytosis was estimated to be equivalent to 0.14 pmol of ATP pr 10(3) mast cells....

  10. Utilization of adenosine triphosphate in rat mast cells during and after secretion of histamine in response to compound 48/80

    DEFF Research Database (Denmark)

    Johansen, Torben


    The adenosine triphosphate (ATP) content of rat mast cells and their lactate production were measured during and after secretion of histamine induced by compound 48/80. Antimycin A and oligomycin were used to block oxidative ATP synthesis, and 2-deoxyglucose (2-DG) was used to block glycolytic ATP...... synthesis. Histamine secretion was completed after 10 sec. exposure of the cells to compound 48/80. During that time period there was an increased ATP-utilization of 0.15 pmol/10(3) cells. After completion of the secretory process there seemed to be an enhanced utilization of ATP of 0.40 pmol/10(3) cells....../min., which may be associated with recovery of the cells....

  11. Effects of bicarbonate buffer on acetylcholine-, adenosine 5'triphosphate-, and cyanide-induced responses in the cat petrosal ganglion in vitro. (United States)

    Soto, Carolina R; Arroyo, Jorge; Alcayaga, Julio


    Acetylcholine (ACh), adenosine 5'-triphosphate (ATP) and sodium cyanide (NaCN) activate petrosal ganglion (PG) neurons in vitro, and evoke ventilatory reflexes in situ, which are abolished after bilateral chemosensory denervation. Because in our previous experiments we superfused the isolated PG with solutions free of CO2/HCO3- buffer, we studied its effects on the PG responses evoked in vitro. PGs from adult cats were superfused at a constant pH, with HEPES-supplemented (5 mM) saline with or without CO2/HCO3- (5%/26.2 mM) buffer, and carotid (sinus) nerve frequency discharge (fCN) recorded. Increases in fCN evoked by ACh, ATP and NaCN in CO2- free saline were significantly reduced (P buffer appears to reduce PG neurons sensitivity to ACh, ATP and NaCN, an effect that may underlie the lack of ventilatory reflexes after bilateral chemodenervation.

  12. Visual and light scattering spectrometric method for the detection of melamine using uracil 5‧-triphosphate sodium modified gold nanoparticles (United States)

    Liang, Lijiao; Zhen, Shujun; Huang, Chengzhi


    A highly selective method was presented for colorimetric determination of melamine using uracil 5‧-triphosphate sodium modified gold nanoparticles (UTP-Au NPs) in this paper. Specific hydrogen-bonding interaction between uracil base (U) and melamine resulted in the aggregation of AuNPs, displaying variations of localized surface plasmon resonance (LSPR) features such as color change from red to blue and enhanced localized surface plasmon resonance light scattering (LSPR-LS) signals. Accordingly, the concentration of melamine could be quantified based on naked eye or a spectrometric method. This method was simple, inexpensive, environmental friendly and highly selective, which has been successfully used for the detection of melamine in pretreated liquid milk products with high recoveries.

  13. Variants of the inosine triphosphate pyrophosphatase gene are associated with reduced relapse risk following treatment for HCV genotype 2/3

    DEFF Research Database (Denmark)

    Rembeck, Karolina; Waldenström, Jesper; Hellstrand, Kristoffer;


    The present study evaluated the impact of variations in the inosine triphosphate pyrophosphatase (ITPase) gene (ITPA) on treatment outcome in patients with hepatitis C virus (HCV) genotype 2/3 infection receiving peginterferon-α2a and lower, conventional 800 mg daily dose of ribavirin. Previous...... naïve HCV genotype 2/3 infected patients, enrolled in a phase III trial (NORDynamIC), were genotyped for ITPA (rs1127354 and rs7270101). Homo- or heterozygosity at Ars1127354 or Crs7270101, entailing reduced ITPase activity, was observed in 37% of patients and was associated with increased likelihood...... duration arms, HCV genotype, fibrosis stage and IL28B genotype, and was not secondary to improved adherence to therapy or less pronounced anemia. Gene variants predicting reduced predicted ITPase activity also were associated with decreased risk of anemia (P

  14. Communication: Near edge x-ray absorption fine structure spectroscopy of aqueous adenosine triphosphate at the carbon and nitrogen K-edges. (United States)

    Kelly, Daniel N; Schwartz, Craig P; Uejio, Janel S; Duffin, Andrew M; England, Alice H; Saykally, Richard J


    Near edge x-ray absorption fine structure (NEXAFS) spectroscopy at the nitrogen and carbon K-edges was used to study the hydration of adenosine triphosphate in liquid microjets. The total electron yield spectra were recorded as a function of concentration, pH, and the presence of sodium, magnesium, and copper ions (Na(+)/Mg(2+)/Cu(2+)). Significant spectral changes were observed upon protonation of the adenine ring, but not under conditions that promote π-stacking, such as high concentration or presence of Mg(2+), indicating that NEXAFS is insensitive to the phenomenon. Intramolecular inner-sphere association of Cu(2+) did create observable broadening of the nitrogen spectrum, whereas outer-sphere association with Mg(2+) did not.

  15. Dielectric spectra broadening as a signature for dipole-matrix interaction. III. Water in adenosine monophosphate/adenosine-5'-triphosphate solutions. (United States)

    Puzenko, Alexander; Levy, Evgeniya; Shendrik, Andrey; Talary, Mark S; Caduff, Andreas; Feldman, Yuri


    In this, the third part of our series on the dielectric spectrum symmetrical broadening of water, we consider the nucleotide aqueous solutions. Where in Parts I [E. Levy et al., J. Chem. Phys. 136, 114502 (2012)] and II [E. Levy et al., J. Chem. Phys. 136, 114503 (2012)], the dipole-dipole or ion-dipole interaction had a dominant feature, now the interplay between these two types of dipole-matrix interactions will be considered. We present the results of high frequency dielectric measurements of different concentrations of adenosine monophosphate/adenosine-5'-triphosphate aqueous solutions. We observed the Cole-Cole broadening of the main relaxation peak of the solvent in the solutions. Moreover, depending on the nucleotide concentration, we observed both types of dipole-matrix interaction. The 3D trajectory approach (described in detail in Part I) is applied in order to highlight the differences between the two types of interaction.

  16. Spontaneous bacterial peritonitis

    Institute of Scientific and Technical Information of China (English)

    Anastasios Koulaouzidis; Shivaram Bhat; Athar A Saeed


    Since its initial description in 1964, research has transformed spontaneous bacterial peritonitis (SBP) from a feared disease (with reported mortality of 90%) to a treatable complication of decompensated cirrhosis,albeit with steady prevalence and a high recurrence rate. Bacterial translocation, the key mechanism in the pathogenesis of SBP, is only possible because of the concurrent failure of defensive mechanisms in cirrhosis.Variants of SBP should be treated. Leucocyte esterase reagent strips have managed to shorten the 'tap-toshot' time, while future studies should look into their combined use with ascitic fluid pH. Third generation cephalosporins are the antibiotic of choice because they have a number of advantages. Renal dysfunction has been shown to be an independent predictor of mortality in patients with SBP. Albumin is felt to reduce the risk of renal impairment by improving effective intravascular volume, and by helping to bind proinflammatory molecules. Following a single episode of SBP, patients should have long-term antibiotic prophylaxis and be considered for liver transplantation.

  17. Interaction of Beta-Hydroxy-Beta-Methylbutyrate Free Acid and Adenosine Triphosphate on Muscle Mass, Strength, and Power in Resistance Trained Individuals. (United States)

    Lowery, Ryan P; Joy, Jordan M; Rathmacher, John A; Baier, Shawn M; Fuller, John C; Shelley, Mack C; Jäger, Ralf; Purpura, Martin; Wilson, Stephanie M C; Wilson, Jacob M


    Lowery, RP, Joy, JM, Rathmacher, JA, Baier, SM, Fuller, JC Jr, Shelley, MC II, Jäger, R, Purpura, M, Wilson, SMC, and Wilson, JM. Interaction of beta-hydroxy-beta-methylbutyrate free acid and adenosine triphosphate on muscle mass, strength, and power in resistance trained individuals. J Strength Cond Res 30(7): 1843-1854, 2016-Adenosine-5'-triphosphate (ATP) supplementation helps maintain performance under high fatiguing contractions and with greater fatigue recovery demands also increase. Current evidence suggests that the free acid form of β-hydroxy-β-methylbutyrate (HMB-FA) acts by speeding regenerative capacity of skeletal muscle after high-intensity or prolonged exercise. Therefore, we investigated the effects of 12 weeks of HMB-FA (3 g) and ATP (400 mg) administration on lean body mass (LBM), strength, and power in trained individuals. A 3-phase double-blind, placebo-, and diet-controlled study was conducted. Phases consisted of an 8-week periodized resistance training program (phase 1), followed by a 2-week overreaching cycle (phase 2), and a 2-week taper (phase 3). Lean body mass was increased by a combination of HMB-FA/ATP by 12.7% (p jump and Wingate power were increased in the HMB-FA/ATP-supplemented group compared with the placebo-supplemented group, and the 12-week increases were 21.5 and 23.7%, respectively. During the overreaching cycle, strength and power declined in the placebo group (4.3-5.7%), whereas supplementation with HMB-FA/ATP resulted in continued strength gains (1.3%). In conclusion, HMB-FA and ATP in combination with resistance exercise training enhanced LBM, power, and strength. In addition, HMB-FA plus ATP blunted the typical response to overreaching, resulting in a further increase in strength during that period. It seems that the combination of HMB-FA/ATP could benefit those who continuously train at high levels such as elite athletes or military personnel.

  18. Antimicrobials for bacterial bioterrorism agents. (United States)

    Sarkar-Tyson, Mitali; Atkins, Helen S


    The limitations of current antimicrobials for highly virulent pathogens considered as potential bioterrorism agents drives the requirement for new antimicrobials that are suitable for use in populations in the event of a deliberate release. Strategies targeting bacterial virulence offer the potential for new countermeasures to combat bacterial bioterrorism agents, including those active against a broad spectrum of pathogens. Although early in the development of antivirulence approaches, inhibitors of bacterial type III secretion systems and cell division mechanisms show promise for the future.

  19. Scanning tunneling microscope assembly, reactor, and system (United States)

    Tao, Feng; Salmeron, Miquel; Somorjai, Gabor A


    An embodiment of a scanning tunneling microscope (STM) reactor includes a pressure vessel, an STM assembly, and three spring coupling objects. The pressure vessel includes a sealable port, an interior, and an exterior. An embodiment of an STM system includes a vacuum chamber, an STM reactor, and three springs. The three springs couple the STM reactor to the vacuum chamber and are operable to suspend the scanning tunneling microscope reactor within the interior of the vacuum chamber during operation of the STM reactor. An embodiment of an STM assembly includes a coarse displacement arrangement, a piezoelectric fine displacement scanning tube coupled to the coarse displacement arrangement, and a receiver. The piezoelectric fine displacement scanning tube is coupled to the coarse displacement arrangement. The receiver is coupled to the piezoelectric scanning tube and is operable to receive a tip holder, and the tip holder is operable to receive a tip.

  20. Tunneling of trapped-atom Bose condensates

    Indian Academy of Sciences (India)

    Subodh R Shenoy


    We obtain the dynamics in number and phase difference, for Bose condensates that tunnel between two wells of a double-well atomic trap, using the (nonlinear) Gross–Pitaevskii equation. The dynamical equations are of the canonical form for the two conjugate variables, and the Hamiltonian corresponds to that of a momentum-shortened pendulum, supporting a richer set of tunneling oscillation modes than for a superconductor Josephson junction, that has a fixed-length pendulum as a mechanical model. Novel modes include ‘inverted pendulum’ oscillations with an average angle of ; and oscillations about a self-maintained population imbalance that we term ‘macroscopic quantum self-trapping’. Other systems with this phase-number nonlinear dynamics include two-component (interconverting) condensates in a single harmonic trap, and He3B superfluids in two containers connected by micropores.

  1. Tunnel backfill erosion by dilute water

    Energy Technology Data Exchange (ETDEWEB)

    Olin, M. [VTT Technical Research Centre of Finland, Espoo (Finland)


    The goal was to estimate smectite release from tunnel backfill due to dilute groundwater pulse during post glacial conditions. The plan was to apply VTT's two different implementations (BESW{sub D} and BESW{sub S}) of well-known model of Neretnieks et al. (2009). It appeared difficult to produce repeatable results using this model in COMSOL 4.2 environment, therefore a semi-analytical approximate approach was applied, which enabled to take into account both different geometry and smectite content in tunnel backfill as compared to buffer case. The results are quite similar to buffer results due to the decreasing effect of smaller smectite content and the increasing effect of larger radius. (orig.)

  2. High-Performance Single Nanowire Tunnel Diodes

    DEFF Research Database (Denmark)

    Wallentin, Jesper; Persson, Johan Mikael; Wagner, Jakob Birkedal;


    We demonstrate single nanowire tunnel diodes with room temperature peak current densities of up to 329 A/cm(2). Despite the large surface to volume ratio of the type-II InP-GaAs axial heterostructure nanowires, we measure peak to valley current ratios (PVCR) of up to 8.2 at room temperature and 2.......6 at liquid helium temperature. These sub-100-nm-diameter structures are promising components for solar cells as well as electronic applications.......We demonstrate single nanowire tunnel diodes with room temperature peak current densities of up to 329 A/cm(2). Despite the large surface to volume ratio of the type-II InP-GaAs axial heterostructure nanowires, we measure peak to valley current ratios (PVCR) of up to 8.2 at room temperature and 27...

  3. Fully magnetic manganite spin filter tunnel junctions (United States)

    Prasad, Bhagwati; Blamire, Mark G.


    In this paper we demonstrate spintronic devices which combine magnetic tunnel junctions with a spin-filtering tunnel barrier. These consist of an ultrathin ferromagnetic insulating barrier, Sm0.75Sr0.25MnO3, sandwiched between two ferromagnetic half-metallic manganite electrodes, La0.7Sr0.3MnO3 and La0.7Ca0.3MnO3, in a nanopillar structure. Depending on the relative magnetic configurations of barrier and electrode layers, three resistance states are well defined, which therefore represent a potential three-state memory concept. These results open the way for the development of spintronic devices by exploiting the many degrees of freedom of perovskite manganite heterostructure systems.

  4. Multiorbital tunneling ionization of the CO molecule

    CERN Document Server

    Wu, J; Kunitski, M; Meckel, M; Voss, S; Sann, H; Kim, H; Jahnke, T; Czasch, A; Dörner, R


    We coincidently measure the molecular frame photoelectron angular distribution and the ion sum-momentum distribution of single and double ionization of CO molecules by using circularly and elliptically polarized femtosecond laser pulses, respectively. The orientation dependent ionization rates for various kinetic energy releases allow us to individually identify the ionizations of multiple orbitals, ranging from the highest occupied to the next two lower-lying molecular orbitals for various channels observed in our experiments. Not only the emission of a single electron, but also the sequential tunneling dynamics of two electrons from multiple orbitals are traced step by step. Our results confirm that the shape of the ionizing orbitals determine the strong laser field tunneling ionization in the CO molecule, whereas the linear Stark effect plays a minor role.

  5. Control of Smoke Flow in a Tunnel

    Directory of Open Access Journals (Sweden)

    K Brahim


    Full Text Available This study focuses on fires in road tunnels in order to increase the level of security for users. This paper proposed numerical investigations carried out on a small scale tunnel model to study the fire-induced smoke control by longitudinal and longitudinal-natural ventilation systems. We studied the effect of two ventilation systems on the temperature distribution and stratification of the pollutant to estimate the effectiveness of ventilation systems. The flow is characterized by the temperature fields, temperature profiles and the Froude number. The numerical tool used is FDS (version 4.0. This numerical study requires validation with experiment and numerical results and comparison with the model developed by Kunsch J.P. to evaluate the critical velocity. However, good agreement with experimental results, it confirms the possibility of using this code in the problem.

  6. Tunnelling matrix elements with Gutzwiller wave functions

    Energy Technology Data Exchange (ETDEWEB)

    Di Ciolo, Andrea; Tocchio, Luca F.; Gros, Claudius [Institut fuer Theoretische Physik, Goethe Universitaet Frankfurt, Frankfurt Am Main (Germany)


    We use a generalized Gutzwiller approach, in order to study projected particle (hole) excitations for superconducting systems and systems with antiferromagnetic (AFM) order. As in the standard Gutzwiller scheme the effects of the strong electronic correlations are given via the suppression of the site double occupancy; for our computations it is helpful to consider a lattice with a reservoir site unaffected by this suppression of the double occupancy. In this approach we obtain the probabilities for the tunnelling of a particle (hole) into the projected state. Our results are due only to the physical properties of the trial state and not to the choice of a specifical Hamiltonian: in this sense, they are model-independent but not universal, because they rely on the features of the chosen Gutzwiller wave function (projected Fermi Sea, BCS superconductor, AFM..) The accuracy and the reliability of our analytical approximation is tested using the Variational Monte Carlo. Possible comparisons with tunnelling experiments are discussed.

  7. View of the LHC tunnel with worker.

    CERN Document Server

    Maximilien Brice


    The 616th dipole out of 1232, on its way to its final position in the tunnel of the LHC. Technicians and engineers continue to work day and night carefully installing 20 magnets a week. Each of the dipoles weighs 34 tonnes and is 15 m long. Once they have been lowered down the specially constructed shaft on the Meyrin site, they begin a slow progression to their final destinations in the LHC tunnel, taking about 10 hours to arrive at Point 6, the furthest point on the LHC ring. Upon arrival, each of the dipoles is aligned and interconnected to the magnets that are already installed.Bigger files available (39Mpx)

  8. Subclinical carpal tunnel syndrome in acromegaly. (United States)

    Kameyama, S; Tanaka, R; Hasegawa, A; Tamura, T; Kuroki, M


    Median nerve conduction was studied in 16 acromegalic patients with asymptomatic carpal tunnel syndrome (CTS) to examine the incidence of subclinical CTS. Thirteen patients (81%) and 23 hands (72%) demonstrated subclinical CTS, 10 bilaterally and three unilaterally in the dominant hand. The incidence reflects the greater sensitivity of the inching method for detecting focal conduction abnormalities. Two of three patients without subclinical CTS showed normal plasma somatomedin-C concentration despite growth hormone hypersecretion. Following adenomectomy, nerve conduction normalized in only six hands (26%). The postoperative persistence of the conduction delay implies that irreversible narrowing of the carpal tunnel rather than reversible soft tissue edema is the principal cause of CTS associated with acromegaly.

  9. Seismic Response of Submerged Floating Tunnel Tether

    Institute of Scientific and Technical Information of China (English)

    SU Zhi-bin; SUN Sheng-nan


    A mathematical equation for vibration of submerged floating tunnel tether under the effects of earthquake and parametric excitation is presented.Multi-step Galerkin method is used to simplify this equation and the fourth-order Runge-Kuta integration method is used for numerical analysis.Finally,vibration response of submerged floating tunnel tether subjected to earthquake and parametric excitation is analyzed in a few numerical examples.The results show that the vibration response of tether varies with the seismic wave type; the steady maximum mid-span displacement of tether subjected to seismic wave keeps constant when parametric resonance takes place; the transient maximum mid-span displacement of tether is related to the peak value of input seismic wave acceleration.

  10. Wind tunnel measurements of windscreen performance (United States)

    Maniet, Edward R.


    Microphone windscreens are routinely used in outdoor acoustic measurements to reduce wind noise pickup. Characterization of windscreen performance outdoors with real wind has several drawbacks notably that the test conditions cannot be controlled. Test methodology has been developed that provides controlled, repeatable measurements of microphone windscreen performance in a laboratory setting. Wind noise measurements are performed using a high-speed/laminar-flow, low-noise wind tunnel that incorporates a large anechoic chamber. The wind tunnel is modified to produce a turbulent flow into which the microphone and windscreen under evaluation are placed. The turbulent velocity spectrum is measured using multi-axis hot-wire anemometers and compared to outdoor data to locate the best position in the turbulent flow to place the test article. Comparative performance measurements of several windscreen designs are presented.

  11. Structural and tunneling properties of Si nanowires

    KAUST Repository

    Montes Muñoz, Enrique


    We investigate the electronic structure and electron transport properties of Si nanowires attached to Au electrodes from first principles using density functional theory and the nonequilibrium Green\\'s function method. We systematically study the dependence of the transport properties on the diameter of the nanowires, on the growth direction, and on the length. At the equilibrium Au-nanowire distance we find strong electronic coupling between the electrodes and nanowires, which results in a low contact resistance. With increasing nanowire length we study the transition from metallic to tunneling conductance for small applied bias. For the tunneling regime we investigate the decay of the conductance with the nanowire length and rationalize the results using the complex band structure of the pristine nanowires. The conductance is found to depend strongly on the growth direction, with nanowires grown along the ⟨110⟩ direction showing the smallest decay with length and the largest conductance and current.

  12. Epigenetics and bacterial infections. (United States)

    Bierne, Hélène; Hamon, Mélanie; Cossart, Pascale


    Epigenetic mechanisms regulate expression of the genome to generate various cell types during development or orchestrate cellular responses to external stimuli. Recent studies highlight that bacteria can affect the chromatin structure and transcriptional program of host cells by influencing diverse epigenetic factors (i.e., histone modifications, DNA methylation, chromatin-associated complexes, noncoding RNAs, and RNA splicing factors). In this article, we first review the molecular bases of the epigenetic language and then describe the current state of research regarding how bacteria can alter epigenetic marks and machineries. Bacterial-induced epigenetic deregulations may affect host cell function either to promote host defense or to allow pathogen persistence. Thus, pathogenic bacteria can be considered as potential epimutagens able to reshape the epigenome. Their effects might generate specific, long-lasting imprints on host cells, leading to a memory of infection that influences immunity and might be at the origin of unexplained diseases.

  13. Bacterial polyhydroxyalkanoates: Still fabulous? (United States)

    Możejko-Ciesielska, Justyna; Kiewisz, Robert


    Bacterial polyhydroxyalkanoates (PHA) are polyesters accumulated as carbon and energy storage materials under limited growth conditions in the presence of excess carbon sources. They have been developed as biomaterials with unique properties for the past many years being considered as a potential substitute for conventional non-degradable plastics. Due to the increasing concern towards global climate change, depleting petroleum resource and problems with an utilization of a growing number of synthetic plastics, PHAs have gained much more attention from industry and research. These environmentally friendly microbial polymers have great potential in biomedical, agricultural, and industrial applications. However, their production on a large scale is still limited. This paper describes the backgrounds of PHAs and discussed the current state of knowledge on the polyhydroxyalkanoates. Ability of bacteria to convert different carbon sources to PHAs, the opportunities and challenges of their introduction to global market as valuable renewable products have been also discussed.

  14. Brownian refrigeration by hybrid tunnel junctions


    Peltonen, J. T.; Helle, M.; Timofeev, A. V.; Solinas, P.; Hekking, F. W. J.; Pekola, Jukka P.


    Voltage fluctuations generated in a hot resistor can cause extraction of heat from a colder normal metal electrode of a hybrid tunnel junction between a normal metal and a superconductor. We extend the analysis presented in Phys. Rev. Lett. 98, 210604 (2007) of this heat rectifying system, bearing resemblance to a Maxwell’s demon. Explicit analytic calculations show that the entropy of the total system is always increasing. We then consider a single-electron transistor configuration with two ...

  15. O(3)-invariant tunneling in general relativity

    Energy Technology Data Exchange (ETDEWEB)

    Berezin, V.A.; Tkachev, I.I.; Kuzmin, V.A.


    We derived a general formula for the action for any O(3)-invariant tunneling processes in false vacuum decay in general relativity. The general classification of the bubble euclidean trajectories is elaborated and explicit expressions for bounces for some processes like the vacuum creation of a double bubble in particular in the vicinity of a black hole, the subbarrier creation of the Einstein-Rosen bridge, creation from nothing of two Minkowski worlds connected by a shell, etc., are given.

  16. Python Engine Installed in Altitude Wind Tunnel (United States)


    An engine mechanic checks instrumentation prior to an investigation of engine operating characteristics and thrust control of a large turboprop engine with counter-rotating propellers under high-altitude flight conditions in the 20-foot-dianieter test section of the Altitude Wind Tunnel at the Lewis Flight Propulsion Laboratory of the National Advisory Committee for Aeronautics, Cleveland, Ohio, now known as the John H. Glenn Research Center at Lewis Field.

  17. Tunneling of electrons through semiconductor superlattices

    Indian Academy of Sciences (India)

    C L Roy


    The purpose of the present paper is to report a study of tunneling of electrons through semiconductor superlattices (SSL); specially, we have analysed diverse features of transmission coefficient of SSL. The SSL we have considered is Ga0.7Al0.3As–GaAs which has been drawing considerable attention during the recent past on account of some typical features of its band structure. We have indicated how our results would help fabrication of ultra high speed devices.

  18. Trophic ulcers in the carpal tunnel syndrome

    Directory of Open Access Journals (Sweden)

    Abelardo Q.-C. Araújo


    Full Text Available A patient with carpal tunnel syndrome (CTS and trophic ulcers is described. Despite the healing of the ulcers after surgery for CTS, the severe sensory deficit and the electrophysiological tests have not shown any significant improvement. We think these findings argue against the hypothesis of the sensory deficit being responsible for the trophic ulcers. We favor a major role for the sympathetic disturbances as the main cause for those lesions.

  19. Seebeck Effect in Magnetic Tunnel Junctions


    Walter, Marvin; Walowski, Jakob; Zbarsky, Vladyslav; Münzenberg, Markus; Schäfers, Markus; Ebke, Daniel; Reiss, Günter; Thomas, Andy; Peretzki, Patrick; Seibt, Michael; Moodera, Jagadeesh S.; Czerner, Michael; Bachmann, Michael; Heiliger, Christian


    Creating temperature gradients in magnetic nanostructures has resulted in a new research direction, i.e., the combination of magneto- and thermoelectric effects. Here, we demonstrate the observation of one important effect of this class: the magneto-Seebeck effect. It is observed when a magnetic configuration changes the charge based Seebeck coefficient. In particular, the Seebeck coefficient changes during the transition from a parallel to an antiparallel magnetic configuration in a tunnel j...

  20. The Femarnbelt Tunnel: Regional Development Perspectives

    DEFF Research Database (Denmark)

    Matthiessen, Christian Wichmann; Lundhus, Peter


    The Fehmarnbelt Link between Denmark and Germany, for which in September 2008 a bilateral government treaty was signed, is the last of the three links uniting transportation networks in Northern Europe. The three links (the Great Belt and the Øresund Link being the other two) are impressive mega...... it was decided that an immersed tunnel should form the basis for the continuous planning of the project, including the environmental impact studies. Completion of the link is scheduled for 2020...

  1. Aeronautical Wind Tunnels, Europe and Asia (United States)


    closed-circuit wind tunnel, 8-kW motor. Operates at sub-freezing temperatures. Built of wood and epoxy-fiberglass composite. Internally insulated with...airplane models. Also used to test wind loads on buildings, bridges, radar antennas, and television towers, and aerodynamic performance of windmills ...flow. This facility has a mahogany six- bladed fan powered by a 2000-hp, 750-V DC motor. At full speed this facility can run at 1.5 MVA. Data

  2. Tunnels: different construction methods and its use for pipelines installation

    Energy Technology Data Exchange (ETDEWEB)

    Mattos, Tales; Soares, Ana Cecilia; Assis, Slow de; Bolsonaro, Ralfo; Sanandres, Simon [Petroleo do Brasil S.A. (PETROBRAS), Rio de Janeiro, RJ (Brazil)


    In a continental dimensions country like Brazil, the pipeline modal faces the challenge of opening ROW's in the most different kind of soils with the most different geomorphology. To safely fulfill the pipeline construction demand, the ROW opening uses all techniques in earthworks and route definition and, where is necessary, no digging techniques like horizontal directional drilling, micro tunneling and also full size tunnels design for pipelines installation in high topography terrains to avoid geotechnical risks. PETROBRAS has already used the tunnel technique to cross higher terrains with great construction difficult, and mainly to make it pipeline maintenance and operation easier. For the GASBOL Project, in Aparados da Serra region and in GASYRG, in Bolivia, two tunnels were opened with approximately 700 meters and 2,000 meters each one. The GASBOL Project had the particularity of being a gallery with only one excavation face, finishing under the hill and from this point was drilled a vertical shaft was drilled until the top to install the pipeline section, while in GASYRG Project the tunnel had two excavation faces. Currently, two projects are under development with tunnels, one of then is the Caraguatatuba-Taubate gas pipeline (GASTAU), with a 5 km tunnel, with the same concepts of the GASBOL tunnel, with a gallery to be opened with the use of a TBM (Tunneling Boring Machine), and a shaft to the surface, and the gas pipeline Cabiunas-Reduc III (GASDUC III) project is under construction with a 3.7 km tunnel, like the GASYRG tunnel with two faces. This paper presents the main excavation tunneling methods, conventional and mechanized, presenting the most relevant characteristics from both and, in particular, the use of tunnels for pipelines installation. (author)

  3. Structure-function analysis of a bacterial deoxyadenosine kinase reveals the basis for substrate specificity. (United States)

    Welin, Martin; Wang, Liya; Eriksson, Staffan; Eklund, Hans


    Deoxyribonucleoside kinases (dNKs) catalyze the transfer of a phosphoryl group from ATP to a deoxyribonucleoside (dN), a key step in DNA precursor synthesis. Recently structural information concerning dNKs has been obtained, but no structure of a bacterial dCK/dGK enzyme is known. Here we report the structure of such an enzyme, represented by deoxyadenosine kinase from Mycoplasma mycoides subsp. mycoides small colony type (Mm-dAK). Superposition of Mm-dAK with its human counterpart's deoxyguanosine kinase (dGK) and deoxycytidine kinase (dCK) reveals that the overall structures are very similar with a few amino acid alterations in the proximity of the active site. To investigate the substrate specificity, Mm-dAK has been crystallized in complex with dATP and dCTP, as well as the products dCMP and dCDP. Both dATP and dCTP bind to the enzyme in a feedback-inhibitory manner with the dN part in the deoxyribonucleoside binding site and the triphosphates in the P-loop. Substrate specificity studies with clinically important nucleoside analogs as well as several phosphate donors were performed. Thus, in this study we combine structural and kinetic data to gain a better understanding of the substrate specificity of the dCK/dGK family of enzymes. The structure of Mm-dAK provides a starting point for making new anti bacterial agents against pathogenic bacteria.

  4. Tunneling ionization of vibrationally excited nitrogen molecules (United States)

    Kornev, Aleksei S.; Zon, Boris A.


    Ionization of molecular nitrogen plays an important role in the process of light-filament formation in air. In the present paper we theoretically investigated tunneling ionization of the valence 3 σg and 1 πu shells in a N2 molecule using a strong near-infrared laser field. This research is based on our previously proposed theory of anti-Stokes-enhanced tunneling ionization with quantum accounting for the vibrationally excited states of the molecules [A. S. Kornev and B. A. Zon, Phys. Rev. A 86, 043401 (2012), 10.1103/PhysRevA.86.043401]. We demonstrated that if the N2 molecule is ionized from the ground vibrational state, then the contribution of the 1 πu orbital is 0.5%. In contrast, for vibrationally excited states with a certain angle between the light polarization vector and the molecule axis, both shells can compete and even reverse their contributions due to the anti-Stokes mechanism. The structure constants of molecular orbitals are extracted from numerical solutions to the Hartree-Fock equations. This approach correctly takes into account the exchange interaction. Quantum consideration of vibrational motion results in the occurrence of the critical vibrational state, the tunneling ionization from which has the maximum rate. The numbers of the critical vibrational states are different for different valence shells. In addition, quantum description of vibrations changes the rate of ionization from the ground vibrational state by 20%-40% in comparison with the quasiclassical results.

  5. Gamma Radiation Tolerance of Magnetic Tunnel Junctions (United States)

    Ren, Fanghui; Jander, Albrecht; Dhagat, Pallavi; Nordman, Cathy


    Determining the radiation tolerance of magnetic tunnel junctions (MTJ), which are the storage elements of non-volatile magnetoresistive random access memories (MRAM), is important for investigating their potential application in space. In this effort, the effect of gamma radiation on MTJs with MgO tunnel barriers was studied. Experimental and control groups of samples were characterized by ex situ measurements of the magnetoresistive hysteresis loops and I-V curves. The experimental group was exposed to gamma rays from a ^60Co source. The samples initially received a dose of 5.9 Mrad (Si) after which they were again characterized electrically and magnetically. Irradiation was then continued for a cumulative dose of 10 Mrad and the devices re-measured. The result shows no change in magnetic properties such as coercivity or exchange coupling due to irradiation. After correcting for differences in temperature at the time of testing, the tunneling magnetoresistance was also found to be unchanged. Thus, it has been determined that MgO-based MTJs are highly tolerant of gamma radiation, particularly in comparison to silicon field-effect transistors which have been shown to degrade with gamma ray exposure even as low as 100 Krad [Zhiyuan Hu. et al., IEEE trans. on Nucl. Sci., vol. 58, 2011].

  6. Energy Optimization of Road Tunnel Lighting Systems

    Directory of Open Access Journals (Sweden)

    Ferdinando Salata


    Full Text Available A road tunnel is an enclosed and covered infrastructure for the vehicular traffic. Its lighting system provides 24 h of artificial sources only, with a higher amount of electric power used during the day. Due to safety reasons, when there is natural lighting outside the tunnel, the lighting levels in the stretches right after the entrance and before the exit must be high, in order to guide the driver’s eye towards the middle of the tunnel where the luminance must guarantee safe driving, avoid any over-dimensioning of the lighting systems, and produce energy savings. Such effects can be reached not only through the technological advances in the field of artificial lighting sources with high luminous efficiency, but also through new materials for road paving characterized by a higher reflection coefficient than other ordinary asphalts. This case study examines different technical scenarios, analyzing and comparing possible energy and economic savings. Traditional solutions are thus compared with scenarios suggesting the solutions previously mentioned. Special asphalts are interesting from an economic point of view, whereas the high costs of LED sources nowadays represent an obstacle for their implementation.

  7. Sensitivity of resonant tunneling diode photodetectors (United States)

    Pfenning, Andreas; Hartmann, Fabian; Langer, Fabian; Kamp, Martin; Höfling, Sven; Worschech, Lukas


    We have studied the sensitivity of AlGaAs/GaAs double barrier resonant tunneling diode photodetectors with an integrated GaInNAs absorption layer for light sensing at the telecommunication wavelength of λ = 1.3 μm for illumination powers from pico- to microwatts. The sensitivity decreases nonlinearly with power. An illumination power increase of seven orders of magnitude leads to a reduction of the photocurrent sensitivity from S I = 5.82 × 103 A W-1 to 3.2 A W-1. We attribute the nonlinear sensitivity-power dependence to an altered local electrostatic potential due to hole-accumulation that on the one hand tunes the tunneling current, but on the other hand affects the lifetime of photogenerated holes. In particular, the lifetime decreases exponentially with increasing hole population. The lifetime reduction results from an enhanced electrical field, a rise of the quasi-Fermi level, and an increased energy splitting within the triangular potential well. The non-constant sensitivity is a direct result of the non-constant lifetime. Based on these findings, we provide an expression that allows us to calculate the sensitivity as a function of illumination power and bias voltage, show a way to model the time-resolved photocurrent, and determine the critical power up to which the resonant tunneling diode photodetector sensitivity can be assumed constant.

  8. Sulphate reduction in the Aespoe HRL tunnel

    Energy Technology Data Exchange (ETDEWEB)

    Gustafson, G. [Chalmers Univ. of Technology, Goeteborg (Sweden). Dept. of Geology; Laaksoharju, M. [ed.; Skaarman, C. [GeoPoint AB, Sollentuna (Sweden); Pedersen, K. [Goeteborg Univ. (Sweden). Dept. of Genetics, Microbiology and Molecular Biology; Rhen, I. [VBB Viak (Sweden); Tullborg, E.L. [Terralogica AB (Sweden); Wallin, B. [Geokema AB (Sweden); Wikberg, P. [Swedish Nuclear Fuel and Waste Management Co., Stockholm (Sweden)


    Evidence and indications of sulphate reduction based on geological, hydrogeological, groundwater, isotope and microbial data gathered in and around the Aespoe Hard Rock Laboratory tunnel have been evaluated. This integrated investigation showed that sulphate reduction had taken place in the past but is most likely also an ongoing process. Anaerobic sulphate-reducing bacteria can live in marine sediments, in the tunnel sections under the sea and in deep groundwaters, since there is no access to oxygen. The sulphate-reducing bacteria seem to thrive when the Cl{sup -} concentration of the groundwater is 4000-6000 mg/l. Sulphate reduction is an in situ process but the resulting hydrogen-sulphide rich water can be transported to other locations. A more vigorous sulphate reduction takes place when the organic content in the groundwater is high (>10 mg/l DOC) which is the case in the sediments and in the groundwaters under the sea. Some bacteria use hydrogen as an electron donor instead of organic carbon and can therefore live in deep environments where access to organic material is limited. The sulphate-reducing bacteria seem to adapt to changing flow situations caused by the tunnel construction relatively fast. Sulphate reduction seems to have occurred and will probably occur where conditions are favourable for the sulphate-reducing bacteria such as anaerobic brackish groundwater with dissolved sulphate and organic carbon or hydrogen. 59 refs, 37 figs, 6 tabs.

  9. An air cleaner for road tunnels

    Institute of Scientific and Technical Information of China (English)


    an air cleaner employing pulse induced plasma chemical process to remove dust and carbon monoxide (CO) in road tunnels is presented, which is composed of mainly a precipitator, a reactor, a flow control system, a power supply and a measurement system. Its performances are studied in simulated air conditions. It is found that the rate of dust removal is dependent on the voltage of the pulse power, the distance between the two dust collecting plates of the electrostatic precipitator,the effective length of the precipitator and the air flow rate in the precipitator, and that of CO removal is affected by the voltage and frequency of the super pulse power, the air flow rate in the reactor and the relative humidity of air. Applying such an cleaner of a proper design to the treatment of polluted air at a flow rate of 7 m/s can achieve the rate of dust removal up to 93 % and that of CO removal up to 72.6 %, which efficiently controls the concentrations of CO and dust under allowable limits. It is implied that the proposed air cleaner is a potential solution to air control in road tunnels, and is prominent for its performances and saving the huge cost of longitudinal ventilation tunnel or vertical vent and ventilation facilities.

  10. Tunneling spectra of graphene on copper unraveled. (United States)

    Zhang, Xin; Stradi, Daniele; Liu, Lei; Luo, Hong; Brandbyge, Mads; Gu, Gong


    Scanning tunneling spectroscopy is often employed to study two-dimensional (2D) materials on conductive growth substrates, in order to gain information on the electronic structures of the 2D material-substrate systems, which can lead to insight into 2D material-substrate interactions, growth mechanisms, etc. The interpretation of the spectra can be complicated, however. Specifically for graphene grown on copper, there have been conflicting reports of tunneling spectra. A clear understanding of the mechanisms behind the variability is desired. In this work, we have revealed that the root cause of the variability in tunneling spectra is the variation in graphene-substrate coupling under various experimental conditions, providing a salutary perspective on the important role of 2D material-substrate interactions. The conclusions are drawn from measured data and theoretical calculations for monolayer, AB-stacked bilayer, and twisted bilayer graphene coexisting on the same substrates in areas with and without intercalated oxygen, demonstrating a high degree of consistency. The Van Hove singularities of the twisted graphene unambiguously indicate the Dirac energy between them, lending strong evidence to our assignment of the spectral features. In addition, we have discovered an O-Cu superstructure that has never been observed before.

  11. Charge Transport in Resonant Tunneling Double - Diodes (United States)

    Diff, Karim

    With the advent of semiconductor devices with typical lengths of the order of a few nanometers and response times of a few picoseconds, the conventional methods used in device modeling have reached their limits of validity. Modern devices based on heterostructures fabricated by Molecular Beam Epitaxy (MBE) require more fundamental approaches based entirely on quantum mechanics. These generally necessitate numerical solutions and are computationally intensive. This dissertation focuses on Resonant Tunneling Double-Barrier (RTDB) diodes as the prototype of "quantum devices". A one-electron model and the effective mass approximation are used. By solving numerically the time-dependent Schrodinger equation for Gaussian wavepackets, the various time characteristics of resonant tunneling are probed. These characteristics are usually overlooked in other treatments based on the time-independent Schrodinger equation. The transit time, the build-up time and the exponential decay time are studied. The difference between these various time scales and their relative importance are discussed. A new method that takes into account the finite extent of the electron wavefunction, is proposed to compute the I-V characteristics of such devices. Results indicate a possible explanation for the discrepancy observed between experimental results and previous analyses. The effect of high frequency fields on resonant tunneling is also studied, and a method to determine the intrinsic cut-off frequency is suggested. The role of the effective mass in the determination of the characteristics of RTDB diodes is emphasized throughout this work.

  12. Chloride Transport in Undersea Concrete Tunnel

    Directory of Open Access Journals (Sweden)

    Yuanzhu Zhang


    Full Text Available Based on water penetration in unsaturated concrete of underwater tunnel, a diffusion-advection theoretical model of chloride in undersea concrete tunnel was proposed. The basic parameters including porosity, saturated hydraulic conductivity, chloride diffusion coefficient, initial water saturation, and moisture retention function of concrete specimens with two water-binder ratios were determined through lab-scale experiments. The variation of chloride concentration with pressuring time, location, solution concentration, initial saturation, hydraulic pressure, and water-binder ratio was investigated through chloride transport tests under external water pressure. In addition, the change and distribution of chloride concentration of isothermal horizontal flow were numerically analyzed using TOUGH2 software. The results show that chloride transport in unsaturated concrete under external water pressure is a combined effect of diffusion and advection instead of diffusion. Chloride concentration increased with increasing solution concentration for diffusion and increased with an increase in water pressure and a decrease in initial saturation for advection. The dominant driving force converted with time and saturation. When predicting the service life of undersea concrete tunnel, it is suggested that advection is taken into consideration; otherwise the durability tends to be unsafe.

  13. High- Speed railway lines in Germany: Experience in Tunnelling in Karstic Geology and Discussion oF Tunnel Driving Methods

    Institute of Scientific and Technical Information of China (English)

    Rudolf Pottler


    In the course of Deutsche Bahn's new high - speed railway line between Nuremberg and Ingolstadt, a number of tunnels are being constructed, with a total length of about 15 km. One of the longest tunnel is the 7,250 m long Irlahüll tunnel.The tunnel was driven in karst geology. It was not expected that karst phenomena would be so widespread. This forced client, designer and contractor to take a new approach to the project. Different tools were developed to handle the problems on site. Additional site investigations had to be performed to identify karst phenomena in the vicinity of the tunnel. For validating the stability and serviceability of the tunnel, a new way of defining the probability of failure of the construction had to be found. Based on the experience of the tunnel driving at the high - speed railway line in Germany between Nuremberg and Ingolstadt, two different methods of tunnelling are discussed: Drill and blast ( New Austrian Tunnelling Method: NATM) and the use of tunnel boring machines (TBM).

  14. Structural Monitoring of Metro Infrastructure during Shield Tunneling Construction

    Directory of Open Access Journals (Sweden)

    L. Ran


    Full Text Available Shield tunneling construction of metro infrastructure will continuously disturb the soils. The ground surface will be subjected to uplift or subsidence due to the deep excavation and the extrusion and consolidation of the soils. Implementation of the simultaneous monitoring with the shield tunnel construction will provide an effective reference in controlling the shield driving, while how to design and implement a safe, economic, and effective structural monitoring system for metro infrastructure is of great importance and necessity. This paper presents the general architecture of the shield construction of metro tunnels as well as the procedure of the artificial ground freezing construction of the metro-tunnel cross-passages. The design principles for metro infrastructure monitoring of the shield tunnel intervals in the Hangzhou Metro Line 1 are introduced. The detailed monitoring items and the specified alarming indices for construction monitoring of the shield tunneling are addressed, and the measured settlement variations at different monitoring locations are also presented.

  15. Self-contained instrument for measuring subterranean tunnel wall deflection (United States)

    Rasmussen, Donald Edgar; Hof, Jr., Peter John


    The deflection of a subterranean tunnel is measured with a rod-like, self-contained instrument that is adapted to be inserted into a radially extending bore of the tunnel adjacent an end of the tunnel where the tunnel is being dug. One end of the instrument is anchored at the end of the bore remote from the tunnel wall, while the other end of the intrument is anchored adjacent the end of the wall in proximity to the tunnel wall. The two ends of the instrument are linearly displaceable relative to each other; the displacement is measured by a transducer means mounted on the instrument. Included in the instrument is a data storage means including a paper tape recorder periodically responsive to a parallel binary signal indicative of the measured displacement.

  16. Addressing student models of energy loss in quantum tunnelling

    CERN Document Server

    Wittmann, M C; Bao, L; Wittmann, Micael C.; Morgan, Jeffrey T.; Bao, Lei


    We report on a multi-year, multi-institution study to investigate student reasoning about energy in the context of quantum tunnelling. We use ungraded surveys, graded examination questions, individual clinical interviews, and multiple-choice exams to build a picture of the types of responses that students typically give. We find that two descriptions of tunnelling through a square barrier are particularly common. Students often state that tunnelling particles lose energy while tunnelling. When sketching wave functions, students also show a shift in the axis of oscillation, as if the height of the axis of oscillation indicated the energy of the particle. We find inconsistencies between students' conceptual, mathematical, and graphical models of quantum tunnelling. As part of a curriculum in quantum physics, we have developed instructional materials to help students develop a more robust and less inconsistent picture of tunnelling, and present data suggesting that we have succeeded in doing so.

  17. Maximum tunneling velocities in symmetric double well potentials

    CERN Document Server

    Manz, Jörn; Schmidt, Burkhard; Yang, Yonggang


    We consider coherent tunneling of one-dimensional model systems in non-cyclic or cyclic symmetric double well potentials. Generic potentials are constructed which allow for analytical estimates of the quantum dynamics in the non-relativistic deep tunneling regime, in terms of the tunneling distance, barrier height and mass (or moment of inertia). For cyclic systems, the results may be scaled to agree well with periodic potentials for which semi-analytical results in terms of Mathieu functions exist. Starting from a wavepacket which is initially localized in one of the potential wells, the subsequent periodic tunneling is associated with tunneling velocities. These velocities (or angular velocities) are evaluated as the ratio of the flux densities versus the probability densities. The maximum velocities are found under the top of the barrier where they scale as the square root of the ratio of barrier height and mass (or moment of inertia), independent of the tunneling distance. They are applied exemplarily to ...

  18. Microwave-induced co-tunneling in single electron tunneling transistors

    DEFF Research Database (Denmark)

    Ejrnaes, M.; Savolainen, M.; Manscher, M.;


    The influence of microwaves on the co-tunneling in single electron tunneling transistors has been investigated as function of frequency and power in the temperature range from 150 to 500 mK. All 20 low frequency connections and the RF line were filtered, and the whole cryostat was suspended...... fixed at maximum Coulomb blockade. With the microwave signal applied to one side of the transistor, we find that the conductance increases linearly with T-2 and microwave power. (C) 2002 Elsevier Science B.V. All rights reserved....

  19. Simulations of Resonant Intraband and Interband Tunneling Spin Filters (United States)

    Ting, David; Cartoixa-Soler, Xavier; McGill, T. C.; Smith, Darryl L.; Schulman, Joel N.


    This viewgraph presentation reviews resonant intraband and interband tunneling spin filters It explores the possibility of building a zero-magnetic-field spin polarizer using nonmagnetic III-V semiconductor heterostructures. It reviews the extensive simulations of quantum transport in asymmetric InAs/GaSb/AlSb resonant tunneling structures with Rashba spin splitting and proposes a. new device concept: side-gated asymmetric Resonant Interband Tunneling Diode (a-RITD).

  20. Quantum tunneling splittings from path-integral molecular dynamics (United States)

    Mátyus, Edit; Wales, David J.; Althorpe, Stuart C.


    We illustrate how path-integral molecular dynamics can be used to calculate ground-state tunnelling splittings in molecules or clusters. The method obtains the splittings from ratios of density matrix elements between the degenerate wells connected by the tunnelling. We propose a simple thermodynamic integration scheme for evaluating these elements. Numerical tests on fully dimensional malonaldehyde yield tunnelling splittings in good overall agreement with the results of diffusion Monte Carlo calculations.

  1. Tunneling Time of Particles Through a Potential Barrier

    Institute of Scientific and Technical Information of China (English)

    LI Chun-Fang; WANG Qi


    A new tunneling time is proposed by introducing the transfer speed of energy carried by tunneling particles. This speed is similar to the energy transfer speed in electromagnetism, and with the tunneling time, the well-known superluminality may be avoided. The low energy limit, the critical limit, and the opaque limit are considered,which are physically meaningful. Comparisons with dwell time and Biittiker and Landauer's semiclassical time are also made.

  2. Giant tunnel-electron injection in nitrogen-doped graphene

    DEFF Research Database (Denmark)

    Lagoute, Jerome; Joucken, Frederic; Repain, Vincent


    Scanning tunneling microscopy experiments have been performed to measure the local electron injection in nitrogen-doped graphene on SiC(000) and were successfully compared to ab initio calculations. In graphene, a gaplike feature is measured around the Fermi level due to a phonon-mediated tunneling...... and at carbon sites. Nitrogen doping can therefore be proposed as a way to improve tunnel-electron injection in graphene....

  3. Fermion Tunnelling of a New Form Finslerian Black Hole

    Institute of Scientific and Technical Information of China (English)

    LIN Kai; YANG Shu-Zheng


    We improve the fermion tunnelling theory proposed by Kerner and Mann, and research into the fermion tunnelling radiation from a Finslerian black hole. The Finsler black hole put forward by Rutz is a solution of Einstein's vacuum field equations in Finsler theory. We study the radiation from the black hole with a semi-classical method, and the result proves that the tunnelling rate depends on the tangent vector.

  4. Shot noise in magnetic tunnel junctions from first principles (United States)

    Liu, Kai; Xia, Ke; Bauer, Gerrit E. W.


    We compute the shot noise in ballistic and disordered Fe|MgO|Fe tunnel junctions by a wave-function-matching method. For tunnel barriers with ≲5 atomic layers we find a suppression of the Fano factor as a function of the magnetic configuration. In the antiparallel configuration the shot noise is full up to a threshold bias that indicates the onset of resonant tunneling. We find excellent agreement with recent experiments when interface disorder is taken into account.

  5. Unification of Filled Function and Tunnelling Function in Global Optimization

    Institute of Scientific and Technical Information of China (English)

    Wei Wang; Yong-jian Yang; Lian-sheng Zhang


    In this paper, two auxiliary functions for global optimization are proposed. These two auxiliary functions possess all characters of tunnelling functions and filled functions under certain general assumptions.Thus, they can be considered as the unification of filled function and tunnelling function. Moreover, the process of tunneling or filling for global optimization can be unified as the minimization of such auxiliary functions.Result of numerical experiments shows that such two auxiliary functions are effective.

  6. Electron tunneling and point contact Andreev reflection studies of superconductors (United States)

    Dai, Wenqing

    The energy gap is the most fundamental property of a superconductor. Electron tunneling spectroscopy and point contact spectroscopy (PCS) are powerful techniques for studying the density of states and energy gap features of superconductors. Two different superconducting systems, multiband superconductor MgB2 and proximity induced topological superconductor NbSe2/Bi 2Se3 heterostructures were studied using either quasiparticle tunneling in planar tunnel junctions or PCS in this work. (Abstract shortened by ProQuest.).



    Hrvoje Antičević; Mario Dobrilović; Hrvoje Perković


    Design of underground spaces, including tunnels, and repositories for radioactive waste include the application of the same or similar technologies. Tunnel excavation by blasting inevitably results in the damage in the rock mass around the excavation profile. The damage in the rock mass immediately next to the tunnel profile emerges as the expanding of the existing cracks and the appearance of new cracks, i.e. as the change of the physical and-mechanical properties of the rock mass. Concernin...

  8. Challenges and solutions in the bioanalysis of BMS-986094 and its metabolites including a highly polar, active nucleoside triphosphate in plasma and tissues using LC-MS/MS. (United States)

    Liu, Ang; Lute, John; Gu, Huidong; Wang, Bonnie; Trouba, Kevin J; Arnold, Mark E; Aubry, Anne-Françoise; Wang, Jian


    BMS-986094, a nucleotide polymerase inhibitor of the hepatitis C virus, was withdrawn from clinical trials because of a serious safety issue. To investigate a potential association between drug/metabolite exposure and toxicity in evaluations conducted after the termination of the BMS-986094 development program, it was essential to determine the levels of BMS-986094 and its major metabolites INX-08032, INX-08144 and INX-09054 in circulation and the active nucleoside triphosphate INX-09114 in target and non-target tissues. However, there were many challenges in the bioanalysis of these compounds. The chromatography challenge for the extremely polar nucleoside triphosphate was solved by applying mixed-mode chromatography which combined anion exchange and reversed-phase interactions. The LC conditions provided adequate retention and good peak shape of the analyte and showed good robustness. A strategy using simultaneous extraction but separate LC analysis of the prodrug BMS-986094 and its major circulating metabolites was used to overcome a carryover issue of the hydrophobic prodrug while still achieving good chromatography of the polar metabolites. In addition, the nucleotide analytes were not stable in the presence of endogenous enzymes. Low pH and low temperature were required for blood collection and plasma sample processing. However, the use of phosphatase inhibitor and immediate homogenization and extraction were critical for the quantitative analysis of the active triphosphate, INX-09114, in tissue samples. To alleviate the bioanalytical complexity caused by multiple analytes, different matrices, and various species, a fit-for-purpose approach to assay validation was implemented based on the needs of drug safety assessment in non-clinical (GLP or non-GLP) studies. The assay for INX-08032 was fully validated in plasma of toxicology species. The lower limit of quantification was 1.00ng/mL and the linear curve range was 1.00-500.00ng/mL using a weighted (1/x(2

  9. Rocket Plume Scaling for Orion Wind Tunnel Testing (United States)

    Brauckmann, Gregory J.; Greathouse, James S.; White, Molly E.


    A wind tunnel test program was undertaken to assess the jet interaction effects caused by the various solid rocket motors used on the Orion Launch Abort Vehicle (LAV). These interactions of the external flowfield and the various rocket plumes can cause localized aerodynamic disturbances yielding significant and highly non-linear control amplifications and attenuations. This paper discusses the scaling methodologies used to model the flight plumes in the wind tunnel using cold air as the simulant gas. Comparisons of predicted flight, predicted wind tunnel, and measured wind tunnel forces-and-moments and plume flowfields are made to assess the effectiveness of the selected scaling methodologies.

  10. Measuring voltage transients with an ultrafast scanning tunneling microscope

    DEFF Research Database (Denmark)

    Keil, Ulrich Dieter Felix; Jensen, Jacob Riis; Hvam, Jørn Märcher


    We use an ultrafast scanning tunneling microscope to resolve propagating voltage transients in space and time. We demonstrate that the previously observed dependence of the transient signal amplitude on the tunneling resistance was only caused by the electrical sampling circuit. With a modified...... circuit, where the tunneling tip is directly connected to the current amplifier of the scanning tunneling microscope, this dependence is eliminated. Ail results can be explained with coupling through the geometrical capacitance of the tip-electrode junction. By illuminating the current...

  11. Atomic Tunnelling Dynamics of Two Squeezed Bose-Einstein Condensates

    Institute of Scientific and Technical Information of China (English)

    LI Jin-Hui; KUANG Le-Man


    In this paper, tunnelling dynamics of squeezed Bose-Einstein condensates (BEC's) in the presence of the nonlinear self-interaction of each species, the interspecies nonlinear interaction, and the Josephson-like tunnelling interaction is investigated by using the second quantization approach. The influence of BEC squeezing on macroscopic quantum self-trapping (MQST) and quantum coherent atomic tunnelling is analyzed in detail. It is shown that the MQST and coherent atomic tunnelling between two squeezed BEC's can be manipulated through changing squeezing amplitude and squeezing phase of BEC squeezed states.

  12. Computational Wind Tunnel: A Design Tool for Rotorcraft Project (United States)

    National Aeronautics and Space Administration — During initial design studies, parametric variation of vehicle geometry is routine. In addition, rotorcraft engineers traditionally use the wind tunnel to evaluate...

  13. Pictorial essay: Role of ultrasound in failed carpal tunnel decompression

    Directory of Open Access Journals (Sweden)

    Rajesh Botchu


    Full Text Available USG has been used for the diagnosis of carpal tunnel syndrome. Scarring and incomplete decompression are the main causes for persistence or recurrence of symptoms. We performed a retrospective study to assess the role of ultrasound in failed carpal tunnel decompression. Of 422 USG studies of the wrist performed at our center over the last 5 years, 14 were for failed carpal tunnel decompression. Scarring was noted in three patients, incomplete decompression in two patients, synovitis in one patient, and an anomalous muscle belly in one patient. No abnormality was detected in seven patients. We present a pictorial review of USG findings in failed carpal tunnel decompression.

  14. Automatic control study of the icing research tunnel refrigeration system (United States)

    Kieffer, Arthur W.; Soeder, Ronald H.


    The Icing Research Tunnel (IRT) at the NASA Lewis Research Center is a subsonic, closed-return atmospheric tunnel. The tunnel includes a heat exchanger and a refrigeration plant to achieve the desired air temperature and a spray system to generate the type of icing conditions that would be encountered by aircraft. At the present time, the tunnel air temperature is controlled by manual adjustment of freon refrigerant flow control valves. An upgrade of this facility calls for these control valves to be adjusted by an automatic controller. The digital computer simulation of the IRT refrigeration plant and the automatic controller that was used in the simulation are discussed.

  15. Superconducting Tunnel Junction Arrays for UV Photon Detection Project (United States)

    National Aeronautics and Space Administration — An innovative method is described for the fabrication of superconducting tunnel junction (STJ) detector arrays offering true "three dimensional" imaging throughout...

  16. Simulation of smoke flow and longitudinal ventilation in tunnel fire

    Institute of Scientific and Technical Information of China (English)

    YANG Gao-shang; AN Yong-lin; PENG Li-min; ZHANG Jin-hua


    Understanding the characteristics of smoke flow in tunnel fire is very important for tunnel safety. The characteristics of tunnel fire were analyzed. The smoke development in different situations of an engineering example was simulated using commercial CFD software PHOENICS 3.5 by field modeling method. The spreading rules and characteristics of concentration field and temperature field of smoke flow with different longitudinal ventilation speeds were studied, which may provide the theoretical background for evacuation design in tunnel fire. The effective measures of fire rescue and crowd evacuation were also described.

  17. Quantum Tunnelling to the Origin and Evolution of Life. (United States)

    Trixler, Frank


    Quantum tunnelling is a phenomenon which becomes relevant at the nanoscale and below. It is a paradox from the classical point of view as it enables elementary particles and atoms to permeate an energetic barrier without the need for sufficient energy to overcome it. Tunnelling might seem to be an exotic process only important for special physical effects and applications such as the Tunnel Diode, Scanning Tunnelling Microscopy (electron tunnelling) or Near-field Optical Microscopy operating in photon tunnelling mode. However, this review demonstrates that tunnelling can do far more, being of vital importance for life: physical and chemical processes which are crucial in theories about the origin and evolution of life can be traced directly back to the effects of quantum tunnelling. These processes include the chemical evolution in stellar interiors and within the cold interstellar medium, prebiotic chemistry in the atmosphere and subsurface of planetary bodies, planetary habitability via insolation and geothermal heat as well as the function of biomolecular nanomachines. This review shows that quantum tunnelling has many highly important implications to the field of molecular and biological evolution, prebiotic chemistry and astrobiology.

  18. Sediment and Cavitation Erosion Studies through Dam Tunnels

    Directory of Open Access Journals (Sweden)

    Muhammad Abid


    Full Text Available This paper presents results of sediment and cavitation erosion through Tunnel 2 and Tunnel 3 of Tarbela Dam in Pakistan. Main bend and main branch of Tunnel 2 and outlet 1 and outlet 3 of Tunnel 3 are concluded to be critical for cavitation and sediment erosion. Studies are also performed for increased sediments flow rate, concluding 5 kg/sec as the critical value for sudden increase in erosion rate density. Erosion rate is concluded to be the function of sediment flow rate and head condition. Particulate mass presently observed is reasonably low, hence presently not affecting the velocity and the flow field.

  19. Cubital tunnel syndrome: A report of two cases

    Directory of Open Access Journals (Sweden)

    Farhana Ebrahim Suleman


    Full Text Available Cubital tunnel syndrome is the second most common peripheral neuropathy of the upper limb. This is due to the anatomy of the tunnel, the physiological changes that the nerve undergoes during elbow flexion, as well as pathological conditions that occur within the tunnel. We present two cases of ulnar neuropathy occurring at the level of the cubital tunnel, demonstrating that this entity may occur owing to an identifiable cause or may show only signal alteration without a visible cause on MRI.

  20. Proceedings of the meeting on tunneling reaction and low temperature chemistry, 97 October. Tunneling reaction and quantum medium

    Energy Technology Data Exchange (ETDEWEB)

    Miyazaki, Tetsuo; Aratono, Yasuyuki; Ichikawa, Tsuneki; Shiotani, Masaru [eds.


    Present report is the proceedings of the 3rd Meeting on Tunneling Reaction and Low Temperature Chemistry held in Oct. 13 and 14, 1997. The main subject of the meeting is `Tunneling Reaction and Quantum Medium`. In the meeting, the physical and chemical phenomena in the liquid helium such as quantum nucleation, spectroscopy of atoms and molecules, and tunneling abstraction reaction of tritium atom were discussed as the main topics as well as the tunneling reactions in the solid hydrogen and organic compounds. Through the meetings held in 1995, 1996, and 1997, the tunneling phenomena proceeding at various temperatures (room temperature to mK) in the wide fields of chemistry, biology, and physics were discussed intensively and the importance of the tunneling phenomena in the science has been getting clear. The 12 of the presented papers are indexed individually. (J.P.N.)