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Sample records for bacterial detachment rates

  1. Direct observation of bacterial deposition on and detachment from nanocomposite membranes embedded with silver nanoparticles.

    Science.gov (United States)

    Liu, Yaolin; Rosenfield, Eric; Hu, Meng; Mi, Baoxia

    2013-06-01

    A microscope-equipped online monitoring system was used to investigate the bacterial deposition and detachment kinetics of a nanocomposite membrane that was synthesized by embedding silver nanoparticles in a polysulfone membrane. A pure polysulfone membrane was used as a control in the experiments. The deposition experiments with live bacteria showed that the bacterial deposition rates were the same for the nanocomposite and control polysulfone membranes. After the rinsing experiments, however, on average a high bacterial detachment ratio of 75% was observed for the nanocomposite membrane, compared with 18% for the control polysulfone membrane. These results indicate that the presence of silver nanoparticles as an antibacterial agent enhances the antiadhesive property of the nanocomposite membrane by decreasing the capability of bacteria in permanently attaching to the membrane surface. A quartz crystal microbalance with dissipation was used to study silver leaching. It was found that silver leaching significantly decreased within the first few hours. Deposition and rinsing experiments with dead bacterial cells revealed that the dead cell deposition rates were similar for both membranes, and so were the detachment ratios. Since the nanocomposite membrane did not show any antiadhesive action against dead cells, its antiadhesive property was most likely attributed to its ability to inhibit biological activities. Results of the antibacterial experiments confirmed that the nanocomposite membrane was highly effective in inhibiting bacterial growth with an antibacterial efficiency of over 98%, which did not decrease even after the membrane was soaked in DI water for seven days.

  2. Quantifying bacterial attachment and detachment using leaching solutions of various ionic strengths after bacterial pulse.

    Science.gov (United States)

    Choi, Nag-Choul; Choi, Jae-Woo; Kwon, Kyu-Sang; Lee, Sang-Gil; Lee, Soonjae

    2017-12-01

    In this study, we quantified the attachment and detachment of bacteria during transport in order to elucidate the contributions of reversible attachment on bacterial breakthrough curves. The first set of breakthrough experiment was performed for a laboratory sand column using leaching solutions of deionized water and mineral salt medium (MSM) of 200 mM with reference to KCl solution by employing Pseudomonas putida as a model bacterium. In the second set of experiment, the ionic strengths of leaching solutions immediately after bacterial pulse were lowered to tenfold and 100-fold diluted system (2 and 20 mM MSM) to focus on the influence of physicochemical factor. Results have shown that bacterial retention occurred in the sand column due to the physical deposition and physicochemical attachment. The physicochemical attachment was attributed to the high ionic strength (200 mM MSM) of leaching solution and the formation of primary energy minimum. Replacing the 200 mM leaching solution with the lower ionic strengths after pulse resulted in the increased tailing of breakthrough curve due to the detachment from the attached bacteria. The detachment could be well explained by DLVO theory, which showed the formation of energy barrier and disappearance of the secondary minimum as the ionic strength gradually decreased. Analysis of mass recovery revealed that 12-20% of the attachment was due to physical and physicochemical attachment, respectively, where the latter consisted of 25-75% of irreversible and reversible attachment respectively.

  3. Analysis of bacterial detachment from substratum surfaces by the passage of air-liquid interfaces

    NARCIS (Netherlands)

    Gomez-Suarez, C; Busscher, HJ; van der Mei, HC

    A theoretical analysis of the detachment of bacteria adhering to substratum surfaces upon the passage of an air-liquid interface is given, together with experimental results for bacterial detachment in the absence and presence of a conditioning film on different substratum surfaces. Bacteria

  4. Influence of cell detachment on the respiration rate of tumor and endothelial cells.

    Science.gov (United States)

    Danhier, Pierre; Copetti, Tamara; De Preter, Géraldine; Leveque, Philippe; Feron, Olivier; Jordan, Bénédicte F; Sonveaux, Pierre; Gallez, Bernard

    2013-01-01

    Cell detachment is a procedure routinely performed in cell culture and a necessary step in many biochemical assays including the determination of oxygen consumption rates (OCR) in vitro. In vivo, cell detachment has been shown to exert profound metabolic influences notably in cancer but also in other pathologies, such as retinal detachment for example. In the present study, we developed and validated a new technique combining electron paramagnetic resonance (EPR) oximetry and the use of cytodex 1 and collagen-coated cytodex 3 dextran microbeads, which allowed the unprecedented comparison of the OCR of adherent and detached cells with high sensitivity. Hence, we demonstrated that both B16F10 melanoma cells and human umbilical vein endothelial cells (HUVEC) experience strong OCR decrease upon trypsin or collagenase treatments. The reduction of cell oxygen consumption was more pronounced with a trypsin compared to a collagenase treatment. Cells remaining in suspension also encounter a marked intracellular ATP depletion and an increase in the lactate production/glucose uptake ratio. These findings highlight the important influence exerted by cell adhesion/detachment on cell respiration, which can be probed with the unprecedented experimental assay that was developed and validated in this study.

  5. Leading Edge Flame Detachment: Effect on Burning Rate of Ammonium Perchlorate Propellants

    Science.gov (United States)

    Price, E. W.; Chakravarthy, S. R.; Sambamurthi, J. K.; Sigman, R. K.

    1997-01-01

    Inner details of the transition from premixed to diffusion controlled burning are examined by considering the transition at the individual particle flamelet level. These considerations lead to prediction of observable singular burning rate behavior of bimodal AP formulations. Burning rate measurements verify the predictions, supporting the view that particle flamelets become detached and retreat to more remote premixed locations at definite (particles size dependent) pressures, revealed by the burning rate tests.

  6. Longtime behavior of one-dimensional biofilm models with shear dependent detachment rates.

    Science.gov (United States)

    Abbas, Fazal; Sudarsan, Rangarajan; Eberl, Hermann J

    2012-04-01

    We investigate the role of non shear stress and shear stressed based detachment rate functions for the longterm behavior of one-dimensional biofilm models. We find that the particular choice of a detachment rate function can affect the model prediction of persistence or washout of the biofilm. Moreover, by comparing biofilms in three settings: (i) Couette flow reactors, (ii) Poiseuille flow with fixed flow rate and (iii) Poiseuille flow with fixed pressure drop, we find that not only the bulk flow Reynolds number but also the particular mechanism driving the flow can play a crucial role for longterm behavior. We treat primarily the single species-case that can be analyzed with elementary ODE techniques. But we show also how the results, to some extent, can be carried over to multi-species biofilm models, and to biofilm models that are embedded in reactor mass balances.

  7. Importance of anisotropy in detachment rates for force production and cargo transport by a team of motor proteins.

    Science.gov (United States)

    Takshak, Anjneya; Kunwar, Ambarish

    2016-05-01

    Many cellular processes are driven by collective forces generated by a team consisting of multiple molecular motor proteins. One aspect that has received less attention is the detachment rate of molecular motors under mechanical force/load. While detachment rate of kinesin motors measured under backward force increases rapidly for forces beyond stall-force; this scenario is just reversed for non-yeast dynein motors where detachment rate from microtubule decreases, exhibiting a catch-bond type behavior. It has been shown recently that yeast dynein responds anisotropically to applied load, i.e. detachment rates are different under forward and backward pulling. Here, we use computational modeling to show that these anisotropic detachment rates might help yeast dynein motors to improve their collective force generation in the absence of catch-bond behavior. We further show that the travel distance of cargos would be longer if detachment rates are anisotropic. Our results suggest that anisotropic detachment rates could be an alternative strategy for motors to improve the transport properties and force production by the team.

  8. Removal of bacterial suspension water occupying the intercellular space of detached leaves after agroinfiltration improves the yield of recombinant hemagglutinin in a Nicotiana benthamiana transient gene expression system.

    Science.gov (United States)

    Fujiuchi, Naomichi; Matsuda, Ryo; Matoba, Nobuyuki; Fujiwara, Kazuhiro

    2016-04-01

    The use of detached leaves instead of whole plants provides an alternative means for recombinant protein production based on Agrobacterium tumefaciens-mediated transient gene overexpression. However, the process for high-level protein production in detached leaves has not yet been established. In this study, we focused on leaf handling and maintenance conditions immediately after infiltration with Agrobacterium suspension (agroinfiltration) to improve recombinant protein expression in detached Nicotiana benthamiana leaves. We demonstrated that the residual water of bacterial suspension in detached leaves had significant impact on the yield of recombinant influenza hemagglutinin (HA). Immediately after agroinfiltration, detached leaves were stored in a dehumidified chamber to allow bacterial suspension water occupying intercellular space to be removed by transpiration. We varied the duration of this water removal treatment from 0.7 to 4.4 h, which resulted in leaf fresh weights ranging from 0.94 to 1.28 g g(-1) relative to weights measured just before agroinfiltration. We used these relative fresh weights (RFWs) as an indicator of the amount of residual water. The detached leaves were then incubated in humidified chambers for 6 days. We found that the presence of residual water significantly decreased HA yield, with a clear inverse correlation observed between HA yield and RFW. We next compared HA yields in detached leaves with those obtained from intact leaves by whole-plant expression performed at the same time. The maximum HA yield obtained from a detached leaf with a RFW of approximately 1.0, namely, 800 μg gFW(-1), was comparable to the mean HA yield of 846 μg gFW(-1) generated in intact leaves. Our results indicate the necessity of removing bacterial suspension water from agroinfiltrated detached leaves in transient overexpression systems and point to a critical factor enabling the detached-leaf system as a viable recombinant protein factory.

  9. Variations in soil detachment rates after wildfire as a function of soil depth, flow properties, and root properties

    Science.gov (United States)

    Moody, John A.; Nyman, Peter

    2013-01-01

    Wildfire affects hillslope erosion through increased surface runoff and increased sediment availability, both of which contribute to large post-fire erosion events. Relations between soil detachment rate, soil depth, flow and root properties, and fire impacts are poorly understood and not represented explicitly in commonly used post-fire erosion models. Detachment rates were measured on intact soil cores using a modified tilting flume. The cores were mounted flush with the flume-bed and a measurement was made on the surface of the core. The core was extruded upward, cut off, and another measurement was repeated at a different depth below the original surface of the core. Intact cores were collected from one site burned by the 2010 Fourmile Canyon (FMC) fire in Colorado and from one site burned by the 2010 Pozo fire in California. Each site contained contrasting vegetation and soil types. Additional soil samples were collected alongside the intact cores and were analyzed in the laboratory for soil properties (organic matter, bulk density, particle-size distribution) and for root properties (root density and root-length density). Particle-size distribution and root properties were different between sites, but sites were similar in terms of bulk density and organic matter. Soil detachment rates had similar relations with non-uniform shear stress and non-uniform unit stream power. Detachment rates within single sampling units displayed a relatively weak and inconsistent relation to flow variables. When averaged across all clusters, the detachment rate displayed a linear relation to shear stress, but variability in soil properties meant that the shear stress accounted for only a small proportion of the overall variability in detachment rates (R2 = 0.23; R2 is the coefficient of determination). Detachment rate was related to root-length density in some clusters (R2 values up to 0.91) and unrelated in others (R2 values 2 value improved and the range of exponents became

  10. Distribution of large-scale detachment faults on mid-ocean ridges in relation to spreading rates

    Institute of Scientific and Technical Information of China (English)

    YU Zhiteng; LI Jiabiao; LIANG Yuyang; HAN Xiqiu; ZHANG Jie; ZHU Lei

    2013-01-01

    Large-scale detachment faults on mid-ocean ridges (MORs) provide a window into the deeper earth. They have megamullion on their corrugated surfaces, with exposed lower crustal and upper mantle rocks, rela-tively high residual Bouguer gravity anomaly and P-wave velocity, and are commonly associated with ocean-ic core complex. According to 30 detachment faults identified on MORs, we found that their distances to the axis mostly range from 5 to 50 km, half-spreading rates range from 6.8 to 17 mm/a, and activity time ranges from recent to 3 Ma. Most of the detachment faults are developed on the slow spreading Mid-Atlantic Ridge (MAR) and ultra-slow spreading Southwest Indian Ridge (SWIR), with the dominant half-spreading rates of 7-13 mm/a, especially 10-13 mm/a. Furthermore, they mostly occur at the inside corner of one segment end and result in an asymmetric seafloor spreading. The detachment faults on MORs are mainly controlled by the tectonism and influenced by the magmatism. Long-lived detachment faults tend to be formed where the ridge magma supply is at a moderate level, although the tectonism is a first-order controlling factor. At the slow spreading ridges, detachment faults tend to occur where local magma supply is relatively low, whilst at the ultra-slow spreading ridges, they normally occur where local magma supply is relatively high. These faults are accompanied by hydrothermal activities, with their relationships being useful in the study of hydrothermal polymetallic sulfides and their origin.

  11. Incidence rates of surgically treated rhegmatogenous retinal detachment among manual workers, non-manual workers and housewives in Tuscany, Italy

    OpenAIRE

    Curti, Stefania; Coggon, David; Baldasseroni, Alberto; Cooke, Robin M. T.; Fresina, Michela; Campos, Emilio C; Semeraro, Francesco; Zanardi, Francesca; Farioli, Andrea; Violante, Francesco S.; Mattioli, Stefano

    2013-01-01

    Purpose Candidate risk factors for idiopathic rhegmatogenous retinal detachment (RRD) include heavy manual handling (requiring Valsalva’s maneuver). We assessed incidence rates of surgically treated idiopathic RRD among manual workers, non-manual workers and housewives resident in Tuscany, Italy. Methods We retrieved all hospital discharge records bearing a principal diagnosis corresponding to RRD coupled with retinal surgery for any resident of Tuscany during 1997–2009. After elimination of ...

  12. The Frank-Starling mechanism is not mediated by changes in rate of cross-bridge detachment.

    Science.gov (United States)

    Wannenburg, T; Janssen, P M; Fan, D; de Tombe, P P

    1997-11-01

    We tested the hypothesis that the Frank-Starling relationship is mediated by changes in the rate of cross-bridge detachment in cardiac muscle. We simultaneously measured isometric force development and the rate of ATP consumption at various levels of Ca2+ activation in skinned rat cardiac trabecular muscles at three sarcomere lengths (2.0, 2.1, and 2.2 microns). The maximum rate of ATP consumption was 1.5 nmol.s-1.microliter fiber vol-1, which represents an estimated adenosinetriphosphatase (ATPase) rate of approximately 10 s-1 per myosin head at 24 degrees C. The rate of ATP consumption was tightly and linearly coupled to the level of isometric force development, and changes in sarcomere length had no effect on the slope of the force-ATPase relationships. The average slope of the force-ATPase relationships was 15.5 pmol.mN-1.mm-1. These results suggest that the mechanisms that underlie the Frank-Starling relationship in cardiac muscle do not involve changes in the kinetics of the apparent detachment step in the cross-bridge cycle.

  13. Detachment and successive re-attachment of multiple, reversibly-binding tethers result in irreversible bacterial adhesion to surfaces

    NARCIS (Netherlands)

    Sjollema, Jelmer; van der Mei, Henny C; Hall, Connie L; Peterson, Brandon W; de Vries, Joop; Song, Lei; Jong, Ed D de; Busscher, Henk J; Swartjes, Jan J T M

    2017-01-01

    Bacterial adhesion to surfaces occurs ubiquitously and is initially reversible, though becoming more irreversible within minutes after first contact with a surface. We here demonstrate for eight bacterial strains comprising four species, that bacteria adhere irreversibly to surfaces through multiple

  14. Divertor detachment

    Science.gov (United States)

    Krasheninnikov, Sergei

    2015-11-01

    The heat exhaust is one of the main conceptual issues of magnetic fusion reactor. In a standard operational regime the large heat flux onto divertor target reaches unacceptable level in any foreseeable reactor design. However, about two decades ago so-called ``detached divertor'' regimes were found. They are characterized by reduced power and plasma flux on divertor targets and look as a promising solution for heat exhaust in future reactors. In particular, it is envisioned that ITER will operate in a partly detached divertor regime. However, even though divertor detachment was studied extensively for two decades, still there are some issues requiring a new look. Among them is the compatibility of detached divertor regime with a good core confinement. For example, ELMy H-mode exhibits a very good core confinement, but large ELMs can ``burn through'' detached divertor and release large amounts of energy on the targets. In addition, detached divertor regimes can be subject to thermal instabilities resulting in the MARFE formation, which, potentially, can cause disruption of the discharge. Finally, often inner and outer divertors detach at different plasma conditions, which can lead to core confinement degradation. Here we discuss basic physics of divertor detachment including different mechanisms of power and momentum loss (ionization, impurity and hydrogen radiation loss, ion-neutral collisions, recombination, and their synergistic effects) and evaluate the roles of different plasma processes in the reduction of the plasma flux; detachment stability; and an impact of ELMs on detachment. We also evaluate an impact of different magnetic and divertor geometries on detachment onset, stability, in- out- asymmetry, and tolerance to the ELMs. Supported by the U.S. Department of Energy Office of Science, Office of Fusion Energy Sciences under Award Number DE-DE-FG02-04ER54739 at UCSD.

  15. Bounds on bacterial cell growth rates

    CERN Document Server

    Landy, Jonathan

    2013-01-01

    Recent experiments have shown that rod-like bacteria in nutrient-rich media grow in length at an exponential rate. Here, I point out that it is the elongated shape of these bacteria that allows for this behavior. Further, I show that when a bacterium's growth is limited by some nutrient -- taken in by the cell through a diffusion-to-capture process -- its growth is suppressed: In three-dimensional geometries, the length $L$ is bounded by $\\log L \\lesssim t^{1/2}$, while in two dimensions the length is bounded by a power-law form. Fits of experimental growth curves to these predicted, sub-exponential forms could allow for direct measures of quantities relating to cellular metabolic rates.

  16. Enteric bacterial growth rates in river water.

    Science.gov (United States)

    Hendricks, C W

    1972-08-01

    Enteric bacteria, including stocked strains of pathogenic species and organisms naturally present in the stream, were capable of growth in a chemostat with autoclaved river water taken 750 m below a sewage outfall. Maximal specific growth rates for all organisms occurred at 30 C, whereas culture generation times ranged between 33.3 and 116 hr. Of the six laboratory strains of enteric species used, Escherichia coli and Enterobacter aerogenes grew at generation times of 34.5 and 33.3 hr, respectively, while the remaining Proteus, Arizona, Salmonella, and Shigella spp. reproduced at a rate two to three times slower than the coliforms. Little or no growth occurred in the water at incubation temperatures of 20 and 5 C, and death was observed for Salmonella senftenberg at 20 and 5 C and for E. aerogenes and Proteus rettgeri at 5 C. When enteric bacteria naturally present in the river water were employed in similar experiments, coliform bacteria demonstrated a generation time of approximately 116 hr, whereas fecal coliforms failed to grow. Growth of the bacteria from the river demonstrated a periodicity of approximately 100 hr, which suggests that much of the growth of these organisms in the chemostat may be on the glass surfaces. This phenomenon, however, was not observed with any of the stocked enteric species. Neither the stock cultures nor the aquatic strains were capable of growth in autoclaved river water taken above the sewage outfall at the three temperatures tested.

  17. Can we estimate bacterial growth rates from ribosomal RNA content?

    Energy Technology Data Exchange (ETDEWEB)

    Kemp, P.F.

    1995-12-31

    Several studies have demonstrated a strong relationship between the quantity of RNA in bacterial cells and their growth rate under laboratory conditions. It may be possible to use this relationship to provide information on the activity of natural bacterial communities, and in particular on growth rate. However, if this approach is to provide reliably interpretable information, the relationship between RNA content and growth rate must be well-understood. In particular, a requisite of such applications is that the relationship must be universal among bacteria, or alternately that the relationship can be determined and measured for specific bacterial taxa. The RNA-growth rate relationship has not been used to evaluate bacterial growth in field studies, although RNA content has been measured in single cells and in bulk extracts of field samples taken from coastal environments. These measurements have been treated as probable indicators of bacterial activity, but have not yet been interpreted as estimators of growth rate. The primary obstacle to such interpretations is a lack of information on biological and environmental factors that affect the RNA-growth rate relationship. In this paper, the available data on the RNA-growth rate relationship in bacteria will be reviewed, including hypotheses regarding the regulation of RNA synthesis and degradation as a function of growth rate and environmental factors; i.e. the basic mechanisms for maintaining RNA content in proportion to growth rate. An assessment of the published laboratory and field data, the current status of this research area, and some of the remaining questions will be presented.

  18. Primary Vitrectomy for Rhegmatogenous Retinal Detachment Associated with Choroidal Detachment

    Institute of Scientific and Technical Information of China (English)

    Xiaohui Zhao; Yiqiao Xing; Ying Chen

    2006-01-01

    Purpose: To evaluate the role and the anatomic and visual results of primary pars plana vitrectomy (PPV) in treating cases with rhegmatogenous retinal detachment associated with choroidal detachment.Methods: All patients were divided into 2 groups. Each group included 23 consecutive eyes with rhegmatogenous retinal detachment and choroidal detachment with proliferative vitreoretinopathy less than grade C. In the study group, controlled removal of vitreous traction was achieved by primary vitrectomy and augmented by scleral buckling if needed. The breaks were treated by focused endolaser coagulation.Postoperative tamponade was done by SF6 or C3F8 gas. In the control group, all patients underwent regular scleral buckling procedure. The cases were followed up for 6 to 12 months.Results: In the study group, retinal reattachment could be achieved in 21 cases (91.30%) after the first operation and in all cases after the second procedure. No occurrence of choroidal detachment occurred after the first procedure. Retinal reattachment rate and visual results tended to be better compared with conventional surgical techniques in the control group.Conclusion: Primary vitrectomy represents a safe, effective method in the management of rhegmatogenous retinal detachment associated with choroidal detachment.

  19. Medium-dependent control of the bacterial growth rate.

    Science.gov (United States)

    Ehrenberg, Måns; Bremer, Hans; Dennis, Patrick P

    2013-04-01

    By combining results from previous studies of nutritional up-shifts we here re-investigate how bacteria adapt to different nutritional environments by adjusting their macromolecular composition for optimal growth. We demonstrate that, in contrast to a commonly held view the macromolecular composition of bacteria does not depend on the growth rate as an independent variable, but on three factors: (i) the genetic background (i.e. the strain used), (ii) the physiological history of the bacteria used for inoculation of a given growth medium, and (iii) the kind of nutrients in the growth medium. These factors determine the ribosome concentration and the average rate of protein synthesis per ribosome, and thus the growth rate. Immediately after a nutritional up-shift, the average number of ribosomes in the bacterial population increases exponentially with time at a rate which eventually is attained as the final post-shift growth rate of all cell components. After a nutritional up-shift from one minimal medium to another minimal medium of higher nutritional quality, ribosome and RNA polymerase syntheses are co-regulated and immediately increase by the same factor equal to the increase in the final growth rate. However, after an up-shift from a minimal medium to a medium containing all 20 amino acids, RNA polymerase and ribosome syntheses are no longer coregulated; a smaller rate of synthesis of RNA polymerase is compensated by a gradual increase in the fraction of free RNA polymerase, possibly due to a gradual saturation of mRNA promoters. We have also analyzed data from a recent publication, in which it was concluded that the macromolecular composition in terms of RNA/protein and RNA/DNA ratios is solely determined by the effector molecule ppGpp. Our analysis indicates that this is true only in special cases and that, in general, medium adaptation also depends on factors other than ppGpp.

  20. Determination of the shear force at the balance between bacterial attachment and detachment in weak-adherence systems, using a flow displacement chamber

    NARCIS (Netherlands)

    Nejadnik, M. Reza; van der Mei, Hermy C.; Busscher, Henk J.; Norde, Willem

    2008-01-01

    We introduce a procedure for determining shear forces at the balance between attachment and detachment of bacteria under flow. This procedure can be applied to derive adhesion forces in weak-adherence systems, such as polymer brush coatings, which are currently at the center of attention for their c

  1. Influence of detachment procedure and diet on recovery of solid-associated bacteria from sheep ruminal digesta and representativeness of bacterial isolates as assessed by automated ribosomal intergenic spacer analysis-polymerase chain reaction.

    Science.gov (United States)

    Ramos, S; Tejido, M L; Ranilla, M J; Martínez, M E; Saro, C; Carro, M D

    2009-11-01

    Six ruminally and duodenally cannulated sheep were used in a partially replicated 4 x 4 Latin square experiment designed to evaluate the efficiency of 3 detachment procedures (DP) to recover solid-associated bacteria (SAB) from ruminal digesta. The 4 experimental diets contained forage to concentrate (F:C) ratios of 70:30 or 30:70 with either alfalfa hay or grass hay as the forage. Bacterial biomass was labeled with 15NH4Cl. The DP were 1) MET: digesta was incubated at 38 degrees C for 15 min with saline solution (0.9% NaCl) containing 0.1% methylcellulose under continuous shaking; 2) STO: digesta was mixed with cold saline solution and homogenized with a stomacher for 5 min at 230 rpm; 3) FRE: digesta was immediately frozen at -20 degrees C for 72 h, thawed at 4 degrees C, mixed with saline solution and subjected to STO procedure. Common to all treatments was storing at 4 degrees C for 24 h after the treatment, homogenization, filtration, and resuspension of digesta 2 times in the treatment solutions. The automated ribosomal intergenic spacer analysis of the 16S ribosomal DNA was used to analyze the similarity between bacterial communities attached to the digesta and those in the pellet obtained after each DP. There were no significant F:C x DP or forage x DP interactions for any variable. On average, STO treatment detached 65.8% of SAB from ruminal digesta, about 1.2 and 1.5 times more than FRE and MET treatments, respectively. Total recovery of SAB in STO pellets (48.9%) was greater compared with FRE (31.7%) and MET (33.1%), values being greater for high-forage compared with high-concentrate diets. Similarity index between the bacteria attached to digesta and those in the pellets were lower for FRE (48.2%) compared with MET (54.1%) and STO (54.1%), which suggests that FRE could have destroyed cell integrity of some bacterial species, thus reducing the bacterial diversity present in the pellets. The STO method was the most effective removing SAB from digesta, but

  2. Adsorption behavior of human plasma fibronectin on hydrophobic and hydrophilic Ti6Al4V substrata and its influence on bacterial adhesion and detachment.

    Science.gov (United States)

    Vadillo-Rodríguez, Virginia; Pacha-Olivenza, Miguel A; Gónzalez-Martín, M Luisa; Bruque, José M; Gallardo-Moreno, Amparo M

    2013-05-01

    Biomaterial implant-associated infections, a common cause of medical devices' failure, are initiated by bacterial adhesion to an adsorbed protein layer on the implant material surface. In this study, the influence of protein surface orientation on bacterial adhesion has been examined using three clinically relevant bacterial strains known to express specific binding sites for human plasma fibronectin (HFN). HFN was allowed to adsorb on hydrophobic Ti6Al4V and physically modified hydrophilic Ti6Al4V substrata. Ellipsometric data reveal that the characteristics of the adsorbed protein layers primary depend on solid surface tension and the initial protein concentration in solution. In particular, HFN molecules adopt a more extended conformation on hydrophobic than hydrophilic surfaces, an effect that is more pronounced at low than at high initial protein concentrations. Moreover, the extended conformation of the protein molecules on these surfaces likely facilitates the exposure of specific sites for adhesion, resulting in the higher bacterial-cell attachment observed regardless of the strain considered. Contact angle measurements and the analysis of the number of remaining adhering cells after being subjected to external forces further suggest that both specific and nonspecific (hydrophobic) interactions play an important role on bacterial attachment. This study is the first one to evaluate the influence of surface hydrophobicity on protein adsorption and its subsequent effect on bacterial adhesion using a material whose hydrophobicity was not modified using chemical treatments that potentially led to surface properties changes other than hydrophobicity.

  3. [Surgical managment of retinal detachment].

    Science.gov (United States)

    Haritoglou, C; Wolf, A

    2015-05-01

    The detachment of the neurosensory retina from the underlying retinal pigment epithelium can be related to breaks of the retina allowing vitreous fluid to gain access to the subretinal space, to exudative changes of the choroid such as tumours or inflammatory diseases or to excessive tractional forces exerted by interactions of the collagenous vitreous and the retina. Tractional retinal detachment is usually treated by vitrectomy and exudative detachment can be addressed by treatment of the underlying condition in many cases. In rhegmatogenous retinal detachment two different surgical procedures, vitrectomy and scleral buckling, can be applied for functional and anatomic rehabilitation of our patients. The choice of the surgical procedure is not really standardised and often depends on the experience of the surgeon and other more ocular factors including lens status, the number of retinal breaks, the extent of the detachment and the amount of preexisting PVR. Using both techniques, anatomic success rates of over 90 % can be achieved. Especially in young phakic patients scleral buckling offers the true advantage to prevent the progression of cataract formation requiring cataract extraction and intraocular lens implantation. Therefore, scleral buckling should be considered in selected cases as an alternative surgical option in spite of the very important technical refinements in modern vitrectomy techniques. Georg Thieme Verlag KG Stuttgart · New York.

  4. Halophilic (aerobic) bacterial growth rate of mangrove ecosystem.

    Science.gov (United States)

    Khan, A Saleem; Ali, M Sheik; Baig, I Juned Ahmed

    2009-09-01

    Mangroves are woody specialized trees of tropics and are valuable flora contributing to economical, ecological, scientific and cultural resources. They thrive in salty environments like coastal regions and are aid towards disaster management facing the onslaught of giant waves such as Tsunami. Analysis of mangrove soil on the banks of the Adyar river behind the Theosophical society campus, Adyar, Chennai, India, gave a startling revelation of microorganisms that can tolerate different salinity ranges. Previous studies in Pichavaram delta, have reported bacterial isolates such as nitrogen fixing bacteria, halophiles and several others. However their efficiency in the growth of mangrove forest has been studied to a lesser extent. The present study has been designed and formulated to estimate halophilic (aerobic) bacterial load from mangroves soil sample based on depth and salinity of the soil and further the efficiency if any of these isolates in the growth of mangroves. Results have been correlated and a cohesive conclusion reached for further intensive research. This study throws light on the ecology of the bacterial population in the coastal marine environment inhabited bymangroves and its possible role in disaster mitigation.

  5. Vision-related quality of life and self-rated satisfaction outcomes of rhegmatogenous retinal detachment surgery: three-year prospective study.

    Directory of Open Access Journals (Sweden)

    Haidong Zou

    Full Text Available BACKGROUND: Subjective functional outcomes measurements, such as vision health-related quality of life (VRQoL and self-rated satisfaction measures can provide helpful multidimensional vision health information that is more comprehensive than traditional objective measures, such as best corrected visual acuity (BCVA. The purpose of this study is to demonstrate 3-year longitudinal postoperative VRQoL and self-rated satisfaction changes after rhegmatogenous retinal detachment (RRD surgery. METHODOLOGY/PRINCIPAL FINDINGS: A prospective case series report was conducted in 92 RRD patients who underwent surgery during January 2004 through December 2006. Preoperative, 3-month, 1-year and 3-year postoperative patient VRQoL and self-rated satisfaction were assessed by face-to-face interviews. The importance of objective variables for predicting three dependent variables: CLVQOL composite scores change, 3-year postoperative CVLQOL composite score and self-rated satisfaction degree scores were calculated by stepwise multivariate linear or logistic regression analysis methods. RESULTS: The total CLVQOL composite scores change ranged between -48 and 90 (mean±standard deviation: 19.48±31.34, including positive changes in 62 patients. The self-rated satisfaction degree scores ultimately improved in 86 patients as compared with preoperative degrees. Statistically significant increases occurred only in the composite scores of subscale mobility and self-rated satisfaction degrees in the first 3 months, while the composite scores of the remaining subscales, and the total CLVQOL, BCVA in the RRD eye and weighted average BCVA, increased steadily throughout the first postoperative year. A better 3-year postoperative weighted average BCVA was associated with all of the 3 dependent outcome variables. CONCLUSIONS: VRQoL of RRD patients improved substantially after surgery and they were satisfied with their postoperative vision. The BCVA, VRQoL and self-rated

  6. Comparison of Heterotrophic Bacterial Production-Rates in Early Spring in the Turbid Estuaries of the Scheldt and the Elbe

    NARCIS (Netherlands)

    Goosen, N.K.; Van Rijswijk, P.; Brockmann, U.

    1995-01-01

    In spring bacterial production rates were estimated by tritiated thymidine incorporation in the turbid estuaries of the rivers Scheldt and Elbe. Bacterial production rates in the Scheldt were 5 times higher than in the Elbe. In the Scheldt bacterial production rates correlated better with the DOC co

  7. Mechanisms and rates of bacterial colonization of sinking aggregates

    DEFF Research Database (Denmark)

    Kiørboe, Thomas; Grossart, H.P.; Ploug, H.;

    2002-01-01

    (0 to 2 s(-1)). The rates at which these bacteria colonized artificial aggregates (stationary and sinking) largely agreed with model predictions. We report several findings. (i) Motile bacteria rapidly colonize aggregates, whereas nonmotile bacteria do not. 00 Flow enhances colonization rates. (iii...... frequency, and turn angles) and the hydrodynamic environment (stationary versus sinking aggregates). We then experimentally tested the models with 10 strains of bacteria isolated from marine particles: two strains were nonmotile; the rest were swimming at 20 to 60 mum s(-1) with different tumbling frequency...

  8. Detachment of multi species biofilm in circulating fluidized bed bioreactor.

    Science.gov (United States)

    Patel, Ajay; Nakhla, George; Zhu, Jingxu

    2005-11-20

    In this study, the detachment rates of various microbial species from the aerobic and anoxic biofilms in a circulating fluidized bed bioreactor (CFBB) with two entirely separate aerobic and anoxic beds were investigated. Overall detachment rate coefficients for biomass, determined on the basis of volatile suspended solids (VSS), glucose and protein as well as for specific microbial groups, i.e., for nitrifiers, denitrifiers, and phosphorous accumulating organisms (PAOs), were established. Biomass detachment rates were found to increase with biomass attachment on carrier media in both beds. The detachment rate coefficients based on VSS were significantly affected by shear stress, whereas for protein, glucose and specific microbial groups, no significant effect of shear stress was observed. High detachment rates were observed for the more porous biofilm structure. The presence of nitrifiers in the anoxic biofilm and denitrifiers in the aerobic biofilm was established by the specific activity measurements. Detachment rates of PAOs in aerobic and anoxic biofilms were evaluated.

  9. Retinal Detachment in Preeclampsia

    Directory of Open Access Journals (Sweden)

    Prado Renata Silva do

    2002-01-01

    Full Text Available Preeclampsia is an obstetric disease of unknown cause that affects approximately 5% of pregnant women. The visual system may be affected with variable intensity, being the retinal detachment a rare complication. The retinal detachment in preeclampsia is usually bilateral and serous, and its pathogenesis is related to the choroidal ischemia secondary to an intense arteriolar vasospasm. The majority of patients have complete recovery of vision with clinical management, and surgery is unnecessary. This is a case report of a 27 year old patient who developed the severe form of preeclampsia on her first pregnancy. She had progressive blurred vision, until she could see only shadows. Ophthalmic examination diagnosed spread and bilateral retinal detachment. With blood pressure control at postpartum, the patient had her retina reattached, and recovery of vision.

  10. Influence of bacterial activities on nitrogen uptake rates determined by the application of antibiotics

    Directory of Open Access Journals (Sweden)

    Clavery Tungaraza

    2003-09-01

    Full Text Available The influence of bacterial activities on inorganic nutrients has always affected total phytoplankton uptake rates owing to the absence of a reliable method that can exclude these effects. The use of natural samples to determine the contribution of bacterial activities has been based on the size fractionation method which, unfortunately, is encumbered with uncertainties, especially because of the size overlap between bacteria and phytoplankton communities. In this paper, the results are reported of an estimation of bacterial activities by the use of inhibitors (antibiotics. It was shown that the contribution of bacterial activities to the uptake of nitrogenous nutrients was highest for ammonium (79%, followed by nitrate (72% and urea (62%. In a second set of experiments the concentration of ammonium was raised by 5 µM. This was done to avoid nutrient limitation resulting from the absence of recycled nutrients following the addition of antibiotics and the maximum contribution of bacterial activity to the uptake rate of ammonium increased to 87%. It can be concluded that the use of inhibitors is a good method, a reliable alternative to the fractionation method. However, it is important to note that inhibitors can affect both phytoplankton growth and the nutrient recycling process. Our results indicate that the application of antibiotics had measurable effects not only on the target bacteria but also on the uptake behaviour of phytoplankton. Our observations were therefore limited to the period when there was no effect on the phytoplankton, as was demonstrated by a carbon protein incorporation experiment.

  11. Bacterial growth on surfaces: Automated image analysis for quantification of growth rate-related parameters

    DEFF Research Database (Denmark)

    Møller, S.; Sternberg, Claus; Poulsen, L. K.

    1995-01-01

    A fast routine method for estimating bacterial cell growth rates by using the metachromatic dye acridine orange is described. The method allows simultaneous estimates of cellular RNA and DNA contents of single cells. Acridine orange staining can be used as a nonspecific supplement to quantitative...

  12. Plasma detachment in linear devices

    Science.gov (United States)

    Ohno, N.

    2017-03-01

    Plasma detachment research in linear devices, sometimes called divertor plasma simulators, is reviewed. Pioneering works exploring the concept of plasma detachment were conducted in linear devices. Linear devices have contributed greatly to the basic understanding of plasma detachment such as volume plasma recombination processes, detached plasma structure associated with particle and energy transport, and other related issues including enhancement of convective plasma transport, dynamic response of plasma detachment, plasma flow reversal, and magnetic field effect. The importance of plasma detachment research using linear devices will be highlighted aimed at the design of future DEMO.

  13. Chlorhexidine Gluconate Dressings Reduce Bacterial Colonization Rates in Epidural and Peripheral Regional Catheters

    Directory of Open Access Journals (Sweden)

    Klaus Kerwat

    2015-01-01

    Full Text Available Introduction. Bacterial colonization of catheter tips is common in regional anesthesia and is a suspected risk factor for infectious complications. This is the first study evaluating the effect of CHG-impregnated dressings on bacterial colonization of regional anesthesia catheters in a routine clinical setting. Methods. In this prospective study, regional anesthesia catheter infection rates were examined in two groups of patients with epidural and peripheral regional catheters. In the first group, regional anesthesia was dressed with a conventional draping. The second group of patients underwent catheter dressing using a CHG-impregnated draping. Removed catheters and the insertion sites were both screened for bacterial colonization. Results. A total of 337 catheters from 308 patients were analysed. There was no significant reduction of local infections in either epidural or peripheral regional anesthesia catheters in both CHG and conventional groups. In the conventional group, 21% of the catheter tips and 41% of the insertion sites showed positive culture results. In the CHG-group, however, only 3% of the catheter tips and 8% of the insertion sites were colonised. Conclusion. CHG dressings significantly reduce bacterial colonization of the tip and the insertion site of epidural and peripheral regional catheters. However, no reductions in rates of local infections were seen.

  14. The rate of change of a soil bacterial community after liming as a function of temperature.

    Science.gov (United States)

    Pettersson, M; Bååth, E

    2003-08-01

    The response of a bacterial community to liming of a forest humus soil (pH 4.9 increased to pH 7.5) was studied in the laboratory at three temperatures (5, 20, and 30 degrees C). As a comparison an unlimed soil (pH 4.9) and a soil limed in the field 15 years ago (pH around 6) were also included. The bacterial community tolerance of pH was measured using TdR incorporation. The pH of the bacterial suspensions (bacteria directly extracted from soil) was altered to 3.6 and 8.3 using different buffers before measuring TdR incorporation. The logarithmic ratio between TdR incorporation at 8.3 and 3.6 was then used as an indicator of the community pH tolerance. The rate of changes in the community tolerance to pH after liming was fastest for the soil incubated at 30 degrees C, but only minor differences in rate of change could be seen between samples incubated at 5 and 20 degrees C. Changes in phospholipid fatty acid (PLFA) pattern after increasing the pH were most rapid for the bacterial community in the soil incubated at 30 degrees C followed by the soil incubated at 20 degrees C, whereas no changes could be seen in the PLFA pattern of the soil incubated at 5 degrees C, even after 82 days' incubation. Thus, the changes in the PLFA pattern were considerably slower than the changes in bacterial community tolerance to pH measured using TdR incorporation.

  15. Between Involvement and Detachment

    DEFF Research Database (Denmark)

    Thomasen, Gry

    a traditional reading of de Gaulle’s policies, and feared that if Gaullist thinking spread among the European allies, it would merit to a return to traditional European power politics. The analysis shows that, by 1964 the administration believed, according to this study, that NATO’s principle of integration......Between Involvement and Detachment takes grasp with the Johnson administration’s (1963-1969) perceptions of and responses to the Western European realignments. Arguing that the Johnson administration set out to maintain the American unilateralist position in the transatlantic relation, not just......, essentially, were detached as America rejected the European reason of state. The Western European realignments were recorded in the Johnson administration with de Gaulle’s critique of US hegemony in Western Europe in the early 1960s. The thesis argues that the administration to a large extent had...

  16. Between Involvement and Detachment

    DEFF Research Database (Denmark)

    Thomasen, Gry

    Between Involvement and Detachment takes grasp with the Johnson administration’s (1963-1969) perceptions of and responses to the Western European realignments. Arguing that the Johnson administration set out to maintain the American unilateralist position in the transatlantic relation, not just a...... the administration maintained. Despite changed circumstances, the Nixon administration’s relation with and perceptions of the European allies largely resemble the traditionalist view of the Johnson administration....

  17. The treatment of bullous rhegmatogenous retinal detachment.

    Science.gov (United States)

    Wong, D; Chignell, A H; Inglesby, D V; Little, B C; Franks, W

    1992-01-01

    We describe the results of a consecutive series of 97 cases of bullous superior retinal detachment treated by conventional surgery. The retinal detachments were characterized by either a single retinal break or multiple retinal breaks confined within 1 clock hour and no proliferative vitreoretinopathy. The surgery involved sequential drainage of subretinal fluid, injection of air, cryotherapy and the application of local explant. All cases would otherwise be suitable for pneumatic retinopexy. The anatomical success rate was 85.5% with a single operation and 97% with further procedures. We report on the complications encountered and appraise the advantages and disadvantages of this operation. Forty-five of the 97 cases had detachment of the macula for less than 2 weeks, and 35 of the 45 (80%) achieved a visual acuity of 6/18 or better. These visual results challenge the assertion that better visual outcome might be attained with pneumatic retinopexy.

  18. Bacterial growth rates are influenced by cellular characteristics of individual species when immersed in electromagnetic fields.

    Science.gov (United States)

    Tessaro, Lucas W E; Murugan, Nirosha J; Persinger, Michael A

    2015-03-01

    Previous studies have shown that exposure to extremely low-frequency electromagnetic fields (ELF-EMFs) have negative effects on the rate of growth of bacteria. In the present study, two Gram-positive and two Gram-negative species were exposed to six magnetic field conditions in broth cultures. Three variations of the 'Thomas' pulsed frequency-modulated pattern; a strong-static "puck" magnet upwards of 5000G in intensity; a pair of these magnets rotating opposite one another at ∼30rpm; and finally a strong dynamic magnetic field generator termed the 'Resonator' with an average intensity of 250μT were used. Growth rate was discerned by optical density (OD) measurements every hour at 600nm. ELF-EMF conditions significantly affected the rates of growth of the bacterial cultures, while the two static magnetic field conditions were not statistically significant. Most interestingly, the 'Resonator' dynamic magnetic field increased the rates of growth of three species (Staphylococcus epidermidis, Staphylococcus aureus, and Escherichia coli), while slowing the growth of one (Serratia marcescens). We suggest that these effects are due to individual biophysical characteristics of the bacterial species.

  19. Effect of microstructure on anomalous strain-rate-dependent behaviour of bacterial cellulose hydrogel.

    Science.gov (United States)

    Gao, Xing; Shi, Zhijun; Lau, Andrew; Liu, Changqin; Yang, Guang; Silberschmidt, Vadim V

    2016-05-01

    This study is focused on anomalous strain-rate-dependent behaviour of bacterial cellulose (BC) hydrogel that can be strain-rate insensitive, hardening, softening, or strain-rate insensitive in various ranges of strain rate. BC hydrogel consists of randomly distributed nanofibres and a large content of free water; thanks to its ideal biocompatibility, it is suitable for biomedical applications. Motivated by its potential applications in complex loading conditions of body environment, its time-dependent behaviour was studied by means of in-aqua uniaxial tension tests at constant temperature of 37 °C at various strain rates ranging from 0.000 1s(-1) to 0.3s(-1). Experimental results reflect anomalous strain-rate-dependent behaviour that was not documented before. Micro-morphological observations allowed identification of deformation mechanisms at low and high strain rates in relation to microstructural changes. Unlike strain-rate softening behaviours in other materials, reorientation of nanofibres and kinematics of free-water flow dominate the softening behaviour of BC hydrogel at high strain rates.

  20. Detachment of solids and nitrifiers in integrated, fixed-film activated sludge systems.

    Science.gov (United States)

    Maas, Carol L A; Parker, Wayne J; Legge, Raymond L

    2008-12-01

    Despite the importance of detachment to biofilm processes, detachment phenomena are not well understood. In this study, researchers investigated biofilm detachment from free-floating biofilm carriers that were established in an integrated, fixed-film activated sludge (IFAS) installation in Mississauga, Ontario. A method for assessing detachment from biofilm carrier systems was devised, evaluated, and refined during this study. In the absence of substrate, superficial air velocity significantly affected the 24-hour detachment rates of total suspended solids from the carriers. Short-term growth conditions did not appear to significantly affect the rate of detachment of solids and nitrifiers. The measured solids-detachment rates were found to be described by a second order function of biofilm attached growth total solids with a detachment coefficient of 0.006 +/- 0.0008 (g/m x d)(-1).

  1. Bacterial Respiration and Growth Rates Affect the Feeding Preferences, Brood Size and Lifespan of Caenorhabditis elegans.

    Directory of Open Access Journals (Sweden)

    Li Yu

    Full Text Available Bacteria serve as live food and nutrients for bacterial-feeding nematodes (BFNs in soils, and influence nematodes behavior and physiology through their metabolism. Five bacterial taxa (Bacillus amyloliquefaciens JX1, Variovorax sp. JX14, Bacillus megaterium JX15, Pseudomonas fluorescens Y1 and Escherichia coli OP50 and the typical BFN Caenorhabditis elegans were selected to study the effects of bacterial respiration and growth rates on the feeding preferences, brood size and lifespan of nematodes. P. fluorescens Y1 and E. coli OP50 were found to be more active, with high respiration and rapid growth, whereas B. amyloliquefaciens JX1 and B. megaterium JX15 were inactive. The nematode C. elegans preferred active P. fluorescens Y1 and E. coli OP50 obviously. Furthermore, worms that fed on these two active bacteria produced more offspring but had shorter lifespan, while inactive and less preferred bacteria had increased nematodes lifespan and decreased the brood size. Based on these results, we propose that the bacterial activity may influence the behavior and life traits of C. elegans in the following ways: (1 active bacteria reproduce rapidly and emit high levels of CO2 attracting C. elegans; (2 these active bacteria use more resources in the nematodes' gut to sustain their survival and reproduction, thereby reducing the worm's lifespan; (3 inactive bacteria may provide less food for worms than active bacteria, thus increasing nematodes lifespan but decreasing their fertility. Nematodes generally require a balance between their preferred foods and beneficial foods, only preferred food may not be beneficial for nematodes.

  2. Bacterial Respiration and Growth Rates Affect the Feeding Preferences, Brood Size and Lifespan of Caenorhabditis elegans.

    Science.gov (United States)

    Yu, Li; Yan, Xiaomei; Ye, Chenglong; Zhao, Haiyan; Chen, Xiaoyun; Hu, Feng; Li, Huixin

    2015-01-01

    Bacteria serve as live food and nutrients for bacterial-feeding nematodes (BFNs) in soils, and influence nematodes behavior and physiology through their metabolism. Five bacterial taxa (Bacillus amyloliquefaciens JX1, Variovorax sp. JX14, Bacillus megaterium JX15, Pseudomonas fluorescens Y1 and Escherichia coli OP50) and the typical BFN Caenorhabditis elegans were selected to study the effects of bacterial respiration and growth rates on the feeding preferences, brood size and lifespan of nematodes. P. fluorescens Y1 and E. coli OP50 were found to be more active, with high respiration and rapid growth, whereas B. amyloliquefaciens JX1 and B. megaterium JX15 were inactive. The nematode C. elegans preferred active P. fluorescens Y1 and E. coli OP50 obviously. Furthermore, worms that fed on these two active bacteria produced more offspring but had shorter lifespan, while inactive and less preferred bacteria had increased nematodes lifespan and decreased the brood size. Based on these results, we propose that the bacterial activity may influence the behavior and life traits of C. elegans in the following ways: (1) active bacteria reproduce rapidly and emit high levels of CO2 attracting C. elegans; (2) these active bacteria use more resources in the nematodes' gut to sustain their survival and reproduction, thereby reducing the worm's lifespan; (3) inactive bacteria may provide less food for worms than active bacteria, thus increasing nematodes lifespan but decreasing their fertility. Nematodes generally require a balance between their preferred foods and beneficial foods, only preferred food may not be beneficial for nematodes.

  3. Conserved rates and patterns of transcription errors across bacterial growth states and lifestyles

    Science.gov (United States)

    Traverse, Charles C.; Ochman, Howard

    2016-01-01

    Errors that occur during transcription have received much less attention than the mutations that occur in DNA because transcription errors are not heritable and usually result in a very limited number of altered proteins. However, transcription error rates are typically several orders of magnitude higher than the mutation rate. Also, individual transcripts can be translated multiple times, so a single error can have substantial effects on the pool of proteins. Transcription errors can also contribute to cellular noise, thereby influencing cell survival under stressful conditions, such as starvation or antibiotic stress. Implementing a method that captures transcription errors genome-wide, we measured the rates and spectra of transcription errors in Escherichia coli and in endosymbionts for which mutation and/or substitution rates are greatly elevated over those of E. coli. Under all tested conditions, across all species, and even for different categories of RNA sequences (mRNA and rRNAs), there were no significant differences in rates of transcription errors, which ranged from 2.3 × 10−5 per nucleotide in mRNA of the endosymbiont Buchnera aphidicola to 5.2 × 10−5 per nucleotide in rRNA of the endosymbiont Carsonella ruddii. The similarity of transcription error rates in these bacterial endosymbionts to that in E. coli (4.63 × 10−5 per nucleotide) is all the more surprising given that genomic erosion has resulted in the loss of transcription fidelity factors in both Buchnera and Carsonella. PMID:26884158

  4. Conserved rates and patterns of transcription errors across bacterial growth states and lifestyles.

    Science.gov (United States)

    Traverse, Charles C; Ochman, Howard

    2016-03-22

    Errors that occur during transcription have received much less attention than the mutations that occur in DNA because transcription errors are not heritable and usually result in a very limited number of altered proteins. However, transcription error rates are typically several orders of magnitude higher than the mutation rate. Also, individual transcripts can be translated multiple times, so a single error can have substantial effects on the pool of proteins. Transcription errors can also contribute to cellular noise, thereby influencing cell survival under stressful conditions, such as starvation or antibiotic stress. Implementing a method that captures transcription errors genome-wide, we measured the rates and spectra of transcription errors in Escherichia coli and in endosymbionts for which mutation and/or substitution rates are greatly elevated over those of E. coli Under all tested conditions, across all species, and even for different categories of RNA sequences (mRNA and rRNAs), there were no significant differences in rates of transcription errors, which ranged from 2.3 × 10(-5) per nucleotide in mRNA of the endosymbiont Buchnera aphidicola to 5.2 × 10(-5) per nucleotide in rRNA of the endosymbiont Carsonella ruddii The similarity of transcription error rates in these bacterial endosymbionts to that in E. coli (4.63 × 10(-5) per nucleotide) is all the more surprising given that genomic erosion has resulted in the loss of transcription fidelity factors in both Buchnera and Carsonella.

  5. 2,4,6-Trinitrotoluene mineralization and bacterial production rates of natural microbial assemblages from coastal sediments

    Energy Technology Data Exchange (ETDEWEB)

    Montgomery, Michael T., E-mail: michael.montgomery@nrl.navy.mil [Naval Research Laboratory, Marine Biogeochemistry Section, Code 6114, 4555 Overlook Avenue, Washington, DC 20375 (United States); Coffin, Richard B., E-mail: richard.coffin@nrl.navy.mil [Naval Research Laboratory, Marine Biogeochemistry Section, Code 6114, 4555 Overlook Avenue, Washington, DC 20375 (United States); Boyd, Thomas J., E-mail: thomas.boyd@nrl.navy.mil [Naval Research Laboratory, Marine Biogeochemistry Section, Code 6114, 4555 Overlook Avenue, Washington, DC 20375 (United States); Smith, Joseph P., E-mail: joseph.smith@nrl.navy.mil [Naval Research Laboratory, Marine Biogeochemistry Section, Code 6114, 4555 Overlook Avenue, Washington, DC 20375 (United States); Walker, Shelby E., E-mail: Shelby.Walker@noaa.gov [Naval Research Laboratory, Marine Biogeochemistry Section, Code 6114, 4555 Overlook Avenue, Washington, DC 20375 (United States); Osburn, Christopher L., E-mail: chris_osburn@ncsu.edu [Marine, Earth and Atmospheric Sciences, North Carolina State University, Raleigh, NC 27695 (United States)

    2011-12-15

    The nitrogenous energetic constituent, 2,4,6-Trinitrotoluene (TNT), is widely reported to be resistant to bacterial mineralization (conversion to CO{sub 2}); however, these studies primarily involve bacterial isolates from freshwater where bacterial production is typically limited by phosphorus. This study involved six surveys of coastal waters adjacent to three biome types: temperate broadleaf, northern coniferous, and tropical. Capacity to catabolize and mineralize TNT ring carbon to CO{sub 2} was a common feature of natural sediment assemblages from these coastal environments (ranging to 270+/-38 {mu}g C kg{sup -1} d{sup -1}). More importantly, these mineralization rates comprised a significant proportion of total heterotrophic production. The finding that most natural assemblages surveyed from these ecosystems can mineralize TNT ring carbon to CO{sub 2} is consistent with recent reports that assemblage components can incorporate TNT ring carbon into bacterial biomass. These data counter the widely held contention that TNT is recalcitrant to bacterial catabolism of the ring carbon in natural environments. - Highlights: > TNT mineralization is a common feature of natural bacterial assemblages in coastal sediments. > TNT mineralization rates comprised a significant proportion of total heterotrophic production. > These data counter the widely held contention that TNT is recalcitrant to bacterial catabolism of the ring carbon in natural environments. - The capacity to mineralize TNT ring carbon to CO{sub 2} is a common feature of natural bacterial assemblages in coastal sediment.

  6. Efficacy of ophthalmic solutions to detach adhering Pseudomonas aeruginosa from contact lenses

    NARCIS (Netherlands)

    Landa, AS; van der Mei, HC; van Rij, G; Busscher, HJ

    1998-01-01

    Purpose. To compare the efficacies of two all-in-one contact lens (CL) cleaning solutions and a detergent mixture on the detachment of a pathogenic bacterium adhering to two types of contact lenses in the absence and presence of a tear film. Methods. Bacterial-detachment studies were carried out in

  7. Electric block current induced detachment from surgical stainless steel and decreased viability of Staphylococcus epidermidis

    NARCIS (Netherlands)

    van der Borden, AJ; van der Mei, HC; Busscher, H

    2005-01-01

    In vitro Studies investigating the influence of electric DC current on bacterial detachment have demonstrated that continuous currents of only 25-125 mu A stimulated Staphylococcal strains to detach from surgical stainless steel. However, DC Currents produce more power that has to be dissipated by t

  8. INFLUENCE OF THE PHYSICAL STATE OF THE BACTERIAL CELL MEMBRANE UPON THE RATE OF RESPIRATION.

    Science.gov (United States)

    HENNEMAN, D H; UMBREIT, W W

    1964-06-01

    Henneman, Dorothy H. (Rutgers, The State University, New Brunswick, N.J.), and W. W. Umbreit. Influence of the physical state of the bacterial cell membrane upon the rate of respiration. J. Bacteriol. 87:1274-1280. 1964.-NaCl and KCl in concentrations of the order of 0.2 to 0.5 m inhibit the respiration of Escherichia coli B and other gram-negative organisms. Cell-free enzymes concerned in respiration and prepared from the same organisms are not inhibited by these salts, whereas these same enzymes tested in intact cells are. The physical state of the cell membrane appears to be a factor controlling its respiratory activity.

  9. Syneresis and delayed detachment in agar plates

    Science.gov (United States)

    Divoux, Thibaut; Mao, Bosi; Snabre, Patrick

    Biogels made of crosslinked polymers such as proteins or polysaccharides behave as porous soft solids and store large amount of solvent. These gels undergo spontaneous aging, called syneresis that consists in the shrinkage of the gel matrix and the progressive expulsion of the solvent. As a result, a biogel originally casted in a container often lose contact with the container sidewalls, and the detachment time is a priori difficult to anticipate since it may occur over variable time spans (from hours to days). Here we report on the syneresis phenomena in agar plates that consist in Petri dishes filled with a gel mainly composed of agar. Direct observations and speckle pattern correlation analysis allow us to rationalize the delayed detachment of the gel from the sidewall of the Petri dish. The detachment time $t^*$ is surprisingly not controlled by the mass loss as one would intuitively expect. Instead, $t^*$ is strongly correlated to the gel minimum thickness $e_{min}$ measured along the sidewall of the plate, and increases as a robust function of $e_{min}$ independently of the prior mass-loss history. Time-resolved correlation spectroscopy atypically applied to such weakly diffusive media gives access to the local thinning rate of the gel. This technique also allows us to detect the gel micro-displacements that are triggered by the water evaporation prior to the detachment, and even to anticipate the latter from a few hours. Our work provides observables to predict the detachment time of agar gels in dishes, and highlights the relevance of speckle pattern correlation analysis for the quantitative investigation of the syneresis dynamics in biopolymer gels.

  10. Syneresis and delayed detachment in agar plates.

    Science.gov (United States)

    Divoux, Thibaut; Mao, Bosi; Snabre, Patrick

    2015-05-14

    Biogels made of crosslinked polymers such as proteins or polysaccharides behave as porous soft solids and store large amounts of solvent. These gels undergo spontaneous aging, called syneresis, which consists of the shrinkage of the gel matrix and the progressive expulsion of solvent. As a result, a biogel originally casted in a container often loses contact with the container sidewalls, and the detachment time is difficult to anticipate a priori, since it may occur over variable time spans (from hours to days). Here we report on syneresis phenomena in agar plates, which consist of Petri dishes filled with a gel mainly composed of agar. Direct observations and speckle pattern correlation analysis allow us to rationalize the delayed detachment of the gel from the sidewall of the Petri dish. The detachment time t* is surprisingly not controlled by the mass loss as one would intuitively expect. Instead, t* is strongly correlated to the gel minimum thickness emin measured along the sidewall of the plate, and increases as a robust function of emin, independently of the prior mass-loss history. Time-resolved correlation spectroscopy atypically applied to such weakly diffusive media gives access to the local thinning rate of the gel. This technique also allows us to detect the gel micro-displacements that are triggered by water evaporation prior to the detachment, and even to anticipate the latter from a few hours. Our work provides observables to predict the detachment time of agar gels in dishes, and highlights the relevance of speckle pattern correlation analysis for the quantitative investigation of the syneresis dynamics in biopolymer gels.

  11. Shock detachment from curved wedges

    Science.gov (United States)

    Mölder, S.

    2017-03-01

    Curved shock theory is used to show that the flow behind attached shocks on doubly curved wedges can have either positive or negative post-shock pressure gradients depending on the freestream Mach number, the wedge angle and the two wedge curvatures. Given enough wedge length, the flow near the leading edge can choke to force the shock to detach from the wedge. This local choking can preempt both the maximum deflection and the sonic criteria for shock detachment. Analytical predictions for detachment by local choking are supported by CFD results.

  12. Shock detachment from curved wedges

    Science.gov (United States)

    Mölder, S.

    2017-09-01

    Curved shock theory is used to show that the flow behind attached shocks on doubly curved wedges can have either positive or negative post-shock pressure gradients depending on the freestream Mach number, the wedge angle and the two wedge curvatures. Given enough wedge length, the flow near the leading edge can choke to force the shock to detach from the wedge. This local choking can preempt both the maximum deflection and the sonic criteria for shock detachment. Analytical predictions for detachment by local choking are supported by CFD results.

  13. Stool consistency is strongly associated with gut microbiota richness and composition, enterotypes and bacterial growth rates.

    Science.gov (United States)

    Vandeputte, Doris; Falony, Gwen; Vieira-Silva, Sara; Tito, Raul Y; Joossens, Marie; Raes, Jeroen

    2016-01-01

    The assessment of potentially confounding factors affecting colon microbiota composition is essential to the identification of robust microbiome based disease markers. Here, we investigate the link between gut microbiota variation and stool consistency using Bristol Stool Scale classification, which reflects faecal water content and activity, and is considered a proxy for intestinal colon transit time. Through 16S rDNA Illumina profiling of faecal samples of 53 healthy women, we evaluated associations between microbiome richness, Bacteroidetes:Firmicutes ratio, enterotypes, and genus abundance with self-reported, Bristol Stool Scale-based stool consistency. Each sample's microbiota growth potential was calculated to test whether transit time acts as a selective force on gut bacterial growth rates. Stool consistency strongly correlates with all known major microbiome markers. It is negatively correlated with species richness, positively associated to the Bacteroidetes:Firmicutes ratio, and linked to Akkermansia and Methanobrevibacter abundance. Enterotypes are distinctly distributed over the BSS-scores. Based on the correlations between microbiota growth potential and stool consistency scores within both enterotypes, we hypothesise that accelerated transit contributes to colon ecosystem differentiation. While shorter transit times can be linked to increased abundance of fast growing species in Ruminococcaceae-Bacteroides samples, hinting to a washout avoidance strategy of faster replication, this trend is absent in Prevotella-enterotyped individuals. Within this enterotype adherence to host tissue therefore appears to be a more likely bacterial strategy to cope with washout. The strength of the associations between stool consistency and species richness, enterotypes and community composition emphasises the crucial importance of stool consistency assessment in gut metagenome-wide association studies. Published by the BMJ Publishing Group Limited. For permission to

  14. Phenomenology of infant death rates. Identification of the peaks of viral and bacterial diseases

    CERN Document Server

    Richmond, Peter

    2016-01-01

    After birth setting up an effective immune system is a major challenge for all living organisms. In this paper we show that this process can be explored by using the age-specific infant death rate as a kind of sensor. This is made possible because, as shown by the authors in Berrut et al. (2016), between birth and a critical age t_c, for all mammals the death rate decreases with age as an hyperbolic function. For humans t_c is equal to 10 years. At some ages the hyperbolic fall displays spikes which, it is assumed, correspond to specific events in the organism's response to exogenous factors. One of these spikes occurs 10 days after birth and there is another at the age of about 300 days. It is shown that the first spike is related to viral infections whereas the second is related to bacterial diseases. By going back to former time periods during which infant mortality was much higher than currently, it is possible to get a magnified view of these peaks which in turn may give us useful information about how a...

  15. Rhegmatogenous retinal detachment: current opinion

    Directory of Open Access Journals (Sweden)

    T. A. Avanesova

    2015-03-01

    Full Text Available Rhegmatogenous retinal detachment (RRD is a severe ocular disorder which requires prompt treatment to prevent low vision and blindness. It is also a significant socio-economic problem as 84% of RDD patients are able-bodied. RRD grading systems (including current Machemer grading system, risk factors, and pathogenesis are reviewed. The role of proliferative vitreoretinopathy in RDD pathogenesis and recurrence is described. Macula involvement determines RDD outcome. Optical coherence tomography (OCT provides the study of retina anatomy and the analysis of parameters that affect post-op best corrected visual acuity, i.e., defects of the junction between inner segments and outer segments (IS/OS, the integrity of external (ELM and internal limiting membrane (ILM, outer nuclear layer thickness (ONLT etc. Fluorescent angiography allows to understand the reasons for low vision in anatomically successful RDD surgery. Scleral buckling, balloon buckling, pneumatic retinopexy, vitrectomy, cryopexy, and laser coagulation are important tools in surgical armamentarium. In recent years, vitrectomy is growing in popularity for RDD treatment. Criteria for procedure selection and surgical success rate in phakic and pseudophakic eyes are discussed. The outcomes of vitrectomy with air/gas and silicone oil tamponade are compared. Bimanual vitrectomy benefits are discussed. 

  16. Rhegmatogenous retinal detachment: current opinion

    Directory of Open Access Journals (Sweden)

    T. A. Avanesova

    2015-01-01

    Full Text Available Rhegmatogenous retinal detachment (RRD is a severe ocular disorder which requires prompt treatment to prevent low vision and blindness. It is also a significant socio-economic problem as 84% of RDD patients are able-bodied. RRD grading systems (including current Machemer grading system, risk factors, and pathogenesis are reviewed. The role of proliferative vitreoretinopathy in RDD pathogenesis and recurrence is described. Macula involvement determines RDD outcome. Optical coherence tomography (OCT provides the study of retina anatomy and the analysis of parameters that affect post-op best corrected visual acuity, i.e., defects of the junction between inner segments and outer segments (IS/OS, the integrity of external (ELM and internal limiting membrane (ILM, outer nuclear layer thickness (ONLT etc. Fluorescent angiography allows to understand the reasons for low vision in anatomically successful RDD surgery. Scleral buckling, balloon buckling, pneumatic retinopexy, vitrectomy, cryopexy, and laser coagulation are important tools in surgical armamentarium. In recent years, vitrectomy is growing in popularity for RDD treatment. Criteria for procedure selection and surgical success rate in phakic and pseudophakic eyes are discussed. The outcomes of vitrectomy with air/gas and silicone oil tamponade are compared. Bimanual vitrectomy benefits are discussed. 

  17. Rates of gyrase supercoiling and transcription elongation control supercoil density in a bacterial chromosome.

    Directory of Open Access Journals (Sweden)

    Nikolay Rovinskiy

    Full Text Available Gyrase catalyzes negative supercoiling of DNA in an ATP-dependent reaction that helps condense bacterial chromosomes into a compact interwound "nucleoid." The supercoil density (σ of prokaryotic DNA occurs in two forms. Diffusible supercoil density (σ(D moves freely around the chromosome in 10 kb domains, and constrained supercoil density (σ(C results from binding abundant proteins that bend, loop, or unwind DNA at many sites. Diffusible and constrained supercoils contribute roughly equally to the total in vivo negative supercoil density of WT cells, so σ = σ(C+σ(D. Unexpectedly, Escherichia coli chromosomes have a 15% higher level of σ compared to Salmonella enterica. To decipher critical mechanisms that can change diffusible supercoil density of chromosomes, we analyzed strains of Salmonella using a 9 kb "supercoil sensor" inserted at ten positions around the genome. The sensor contains a complete Lac operon flanked by directly repeated resolvase binding sites, and the sensor can monitor both supercoil density and transcription elongation rates in WT and mutant strains. RNA transcription caused (- supercoiling to increase upstream and decrease downstream of highly expressed genes. Excess upstream supercoiling was relaxed by Topo I, and gyrase replenished downstream supercoil losses to maintain an equilibrium state. Strains with TS gyrase mutations growing at permissive temperature exhibited significant supercoil losses varying from 30% of WT levels to a total loss of σ(D at most chromosome locations. Supercoil losses were influenced by transcription because addition of rifampicin (Rif caused supercoil density to rebound throughout the chromosome. Gyrase mutants that caused dramatic supercoil losses also reduced the transcription elongation rates throughout the genome. The observed link between RNA polymerase elongation speed and gyrase turnover suggests that bacteria with fast growth rates may generate higher supercoil densities

  18. Spectroscopic investigations of divertor detachment in TCV

    CERN Document Server

    Verhaegh, K; Duval, B P; Harrison, J R; Reimerdes, H; Theiler, C; Labit, B; Maurizio, R; Marini, C; Nespoli, F; Sheikh, U; Tsui, C K; Vianello, N; Vijvers, W A J

    2016-01-01

    The aim of this work is to provide an understanding of detachment at TCV with emphasis on analysis of the Balmer line emission. A new Divertor Spectroscopy System has been developed for this purpose. Further development of Balmer line analysis techniques has allowed detailed information to be extracted on free-free and three-body recombination. During density ramps, the plasma at the target detaches as inferred from a drop in density at, and ion current to, the target. At the same time the Balmer $6\\rightarrow2$ and $7\\rightarrow2$ line emission near the target is dominated by recombination, indicating that the ionization region has also detached from the target to be replaced by a recombining region with densities more than a factor 2 higher than at the target. As the core density increases further, the density and recombination rate are rising all along the outer leg to the x-point while remaining highest at the target. Even at the highest core densities accessed (Greenwald fraction 0.7) the peaks in recomb...

  19. CELL DETACHMENT BY PROLYL-SPECIFIC ENDOPEPTIDASE FROM WOLFIPORIA COCOS

    Directory of Open Access Journals (Sweden)

    Katharina Cierpka

    2014-01-01

    Full Text Available As requirements for Advanced Therapy Medicinal Product (ATMP production differ from other production processes (e.g., therapeutic protein production, cell detachment is often a crucial step for the process success. In most cases, cell detachment is done enzymatically. Although many peptidases are established in cell culture in R&D, e.g., Trypsin as gold standard, many of them seem to be unsuitable in ATMP production processes. Therefore, the present study investigated a novel endopeptidase used in food biotechnology for its applicability in ATMP processes where cell detachment is needed. The Prolyl-specific Peptidase (PsP is of non-mammalian origin and considered as safe for humans. PsP was purified from the supernatant of the fungus Wolfiporia cocos. The isolation and purification resulted in an enzyme solution with 0.19 U mg-1 prolyl-specific activity. By in silico analysis it was confirmed that attachment-promoting proteins can be cleaved by PsP in a similar amount than with Trypsin. Further the proteolytic activity was determined for PsP and Trypsin by using the same enzymatic assay. Detachment with both enzymes was compared for cells used in typical therapeutic production processes namely a mesenchymal stem cell line (hMSC-TERT as a model for a cell therapeutic, Vero and MA104 cells used for viral therapeutic or vaccine production. The cell detachment experiments were performed with comparable enzyme activities (1.6 U mL-1. hMSC-TERT detachment was faster with PsP than with Trypsin. For Vero cells the detachment with PsP was not only faster but also more efficient. For MA104 cells the detachment rate with PsP was similar to Trypsin. For all cell types, detachment with PsP showed less influence on cell growth and metabolism compared to standard Trypsin.Thus, three cell types used in ATMP, viral therapeutics or vaccine production can be detached efficiently and gently with PsP. Therefore, PsP shows

  20. Retinal detachment surgery without cryotherapy.

    OpenAIRE

    Chignell, A H; Markham, R H

    1981-01-01

    A series of cases of retinal detachment treated without the application of cryotherapy at the time of surgery has been studied. The omission of cryotherapy while not interfering with retinal reattachment, carries the risk of redetachment at a later date. Macular pucker may still occur in spite of the absence of cryotherapy.

  1. The mechanics of retinal detachment

    Science.gov (United States)

    Chou, Tom; Siegel, Michael

    2013-03-01

    We present a model of the mechanical and fluid forces associated with exudative retinal detachments where the retinal photoreceptor cells separate typically from the underlying retinal pigment epithelium (RPE). By computing the total fluid volume flow arising from transretinal, vascular, and retinal pigment epithelium (RPE) pump currents, we determine the conditions under which the subretinal fluid pressure exceeds the maximum yield stress holding the retina and RPE together, giving rise to an irreversible, extended retinal delamination. We also investigate localized, blister-like retinal detachments by balancing mechanical tension in the retina with both the retina-RPE adhesion energy and the hydraulic pressure jump across the retina. For detachments induced by traction forces, we find a critical radius beyond which the blister is unstable to growth. Growth of a detached blister can also be driven by inflamed tissue within which e.g., the hydraulic conductivities of the retina or choroid increase, the RPE pumps fail, or the adhesion properties change. We determine the parameter regimes in which the blister either becomes unstable to growth, remains stable and finite-sized, or shrinks, allowing possible healing. This work supported by the Army Research Office through grant 58386MA

  2. Characterization of the rate and temperature sensitivities of bacterial remineralization of dissolved organic phosphorus by natural populations

    Directory of Open Access Journals (Sweden)

    Angelicque E. White

    2012-08-01

    Full Text Available Production, transformation, and degradation are the principal components of the cycling of dissolved organic matter (DOM in marine systems. Heterotrophic Bacteria (and Archaea play a large part in this cycling via enzymatic decomposition and intracellular transformations of organic material to inorganic carbon (C, nitrogen (N , and phosphorus (P. The rate and magnitude of inorganic nutrient regeneration from DOM is related to the elemental composition and lability of DOM substrates as well as the nutritional needs of the mediating organisms. While many previous efforts have focused on C and N cycling of DOM, less is known in regards to the controls of organic P utilization and remineralization by natural populations of bacteria. In order to constrain the relative time scales and degradation of select dissolved organic P (DOP compounds we have conducted a series of experiments focused on (1 assessment of the short-term lability of a range of DOP compounds, (2 characterization of labile DOP remineralization rates and (3 examination of temperature sensitivities of labile DOP remineralization for varying bacterial populations. Results reinforce previous findings of monoester and polyphosphate lability and the relative recalcitrance of a model phosphonate: 2-aminoethylphosphonate. High resolution time-series of P monoester remineralization indicates decay constants on the order of 0.67-7.04 d-1 for bacterial populations isolated from coastal and open ocean surface waters. The variability of these rates is predictably related to incubation temperature and initial concentrations of heterotrophic bacteria. Additional controls on DOP hydrolysis included seasonal shifts in bacterial populations and the physiological state of bacteria at the initiation of DOP addition experiments. Composite results indicate that bacterial hydrolysis of P-monoesters exceeds bacterial P demand and thus DOP remineralization efficiency may control P availability to autotrophs.

  3. Bacterial diversity in five Icelandic geothermal waters: temperature and sinter growth rate effects

    OpenAIRE

    D.J. Tobler; Liane G. Benning

    2011-01-01

    The microbial ecology associated with siliceous sinters was studied in five geochemically diverse Icelandic geothermal systems. Bacterial 16S rRNA clone libraries were constructed from water-saturated precipitates from each site resulting in a total of 342 bacterial clone sequences and 43 species level phylotypes. In near-neutral, saline (2.6-4.7% salinity) geothermal waters where sinter growth varied between 10 and similar to 300 kg year(-1) m(-2), 16S rRNA gene analyses revealed very low (n...

  4. Organic loading rates affect composition of soil-derived bacterial communities during continuous, fermentative biohydrogen production

    Energy Technology Data Exchange (ETDEWEB)

    Luo, Yonghua; Bruns, Mary Ann [Department of Crop and Soil Sciences, The Pennsylvania State University, University Park, PA 16802 (United States); Zhang, Husen; Salerno, Michael; Logan, Bruce E. [Department of Civil and Environmental Engineering, The Pennsylvania State University, University Park, PA 16802 (United States)

    2008-11-15

    Bacterial community composition during steady-state, fermentative H{sub 2} production was compared across a range of organic loading rates (OLRs) of 0.5-19 g COD l{sup -1} h{sup -1} in a 2-l continuous flow reactor at 30 C. The varied OLRs were achieved with glucose concentrations of 2.5-10 g l{sup -1} and hydraulic retention times of 1-10 h. The synthetic wastewater feed was amended with L-cysteine and maintained at a pH of 5.5. For each run at a given glucose concentration, the reactor was inoculated with an aliquot of well-mixed agricultural topsoil that had been heat-treated to reduce numbers of vegetative cells. At OLRs less than 2 g COD l{sup -1} h{sup -1}, DNA sequences from ribosomal RNA intergenic spacer analysis profiles revealed more diverse and variable populations (Selenomonas, Enterobacter, and Clostridium spp.) than were observed above 2 g COD l{sup -1} h{sup -1} (Clostridium spp. only). An isolate, LYH1, was cultured from a reactor sample (10 g glucose l{sup -1} at a 10-h HRT) on medium containing L-cysteine. In confirming H{sub 2} production by LYH1 in liquid batch culture, lag periods for H{sub 2} production in the presence and absence of L-cysteine were 5 and 50 h, respectively. The 16S rRNA gene sequence of LYH1 indicated that the isolate was a Clostridium sp. affiliated with RNA subcluster Ic, with >99% similarity to Clostridium sp. FRB1. In fluorescent in situ hybridization tests, an oligonucleotide probe complementary to the 16S rRNA of LYH1 hybridized with 90% of cells observed at an OLR of 2 g COD h{sup -1}, compared to 26% of cells at an OLR of 0.5 g COD l{sup -1} h{sup -1}. An OLR of 2 g COD l{sup -1} h{sup -1} appeared to be a critical threshold above which clostridia were better able to outcompete Enterobacteriaceae and other organisms in the mixed soil inoculum. Our results are discussed in light of other biohydrogen studies employing pure cultures and mixed inocula. (author)

  5. Antimicrobials Influence Bond Stiffness and Detachment of Oral Bacteria.

    Science.gov (United States)

    Song, L; Hou, J; van der Mei, H C; Veeregowda, D H; Busscher, H J; Sjollema, J

    2016-07-01

    Oral biofilm can never be fully removed by oral hygiene measures. Biofilm left behind after brushing is often left behind on the same sites and exposed multiple times to antimicrobials from toothpastes and mouthrinses, after which removal becomes increasingly difficult. On the basis of this observation, we hypothesize that oral bacteria adhering to salivary conditioning films become more difficult to remove after adsorption of antimicrobials due to stiffening of their adhesive bond. To verify this hypothesis, bacteria adhering to bare and saliva-coated glass were exposed to 3 different mouthrinses, containing chlorhexidine-digluconate, cetylpyridinium-chloride, or amine-fluoride, after which bacterial vibration spectroscopy was carried out or a liquid-air interface was passed over the adhering bacteria to stimulate their detachment. Brownian motion-induced nanoscopic vibration amplitudes of 4 oral streptococcal strains, reflecting their bond stiffness, decreased after exposure to mouthrinses. Concurrently, the percentage detachment of adhering bacteria upon the passage of a liquid-air interface decreased after exposure to mouthrinses. A buffer control left both vibration amplitudes and detachment percentages unaffected. Exposure to either of the selected mouthrinses yielded more positively charged bacteria by particulate microelectrophoresis, suggesting antimicrobial adsorption to bacterial cell surface components. To rule out that exposure of adhering bacteria to the mouthrinses stimulated polysaccharide production with an impact on their detachment, Fourier transform infrared spectroscopy was carried out on bacteria adhering to an internal reflection element, prior to and after exposure to the mouthrinses. Infrared absorption band areas indicated no significant change in amount of polysaccharides after exposure of adhering bacteria to mouthrinses, but wave number shifts demonstrated stiffening of polysaccharides in the bond, as a result of antimicrobial

  6. Evaluation of bacterial contamination rate of the anterior chamber during phacoemulsification surgery using an automated microbial detection system

    Institute of Scientific and Technical Information of China (English)

    Ibrahim; Kocak; Funda; Kocak; Bahri; Teker; Ali; Aydin; Faruk; Kaya; Hakan; Baybora

    2014-01-01

    ·AIM: To assess the incidence of anterior chamber bacterial contamination during phacoemulsification surgery using an automated microbial detection system(BacT/Alert).·METHODS: Sixty-nine eyes of 60 patients who had uneventful phacoemulsification surgery, enrolled in this prospective study. No prophylactic topical or systemic antibiotics were used before surgery. After antisepsis with povidone-iodine, two intraoperative anterior chamber aqueous samples were obtained, the first whilst entering anterior chamber, and the second at the end of surgery. BacT/Alert culture system was used to detect bacterial contamination in the aqueous samples.·RESULTS: Neither aqueous samples obtained at the beginning nor conclusion of the surgery was positive for microorganisms on BacT/Alert culture system. The rate of bacterial contamination during surgery was 0%. None of the eyes developed acute-onset endophthalmitis after surgery.· CONCLUSION: In this study, no bacterial contamination of anterior chamber was observed during cataract surgery. This result shows that meticulous surgical preparation and technique can prevent anterior chamber contamination during phacoemulsification cataract surgery.

  7. Detached Growth of Germanium by Directional Solidification

    Science.gov (United States)

    Palosz, W.; Volz, M. P.; Cobb, S.; Motakef, S.; Szofran, F. R.

    2004-01-01

    The conditions of detached solidification under controlled pressure differential across the meniscus were investigated. Uncoated and graphite- or BN-coated silica and pBN crucibles were used. Detached and partly detached growth was achieved in pBN and BN-coated crucibles, respectively. The results of the experiments are discussed based on the theory of Duffar et al.

  8. Selective detachment process in column flotation froth

    Energy Technology Data Exchange (ETDEWEB)

    Honaker, R.Q.; Ozsever, A.V.; Parekh, B.K. [University of Kentucky, Lexington, KY (United States). Dept. of Mining Engineering

    2006-05-15

    The selectivity in flotation columns involving the separation of particles of varying degrees of floatability is based on differential flotation rates in the collection zone, reflux action between the froth and collection zones, and differential detachment rates in the froth zone. Using well-known theoretical models describing the separation process and experimental data, froth zone and overall flotation recovery values were quantified for particles in an anthracite coal that have a wide range of floatability potential. For highly floatable particles, froth recovery had a very minimal impact on overall recovery while the recovery of weakly floatable material was decreased substantially by reductions in froth recovery values. In addition, under carrying-capacity limiting conditions, selectivity was enhanced by the preferential detachment of the weakly floatable material. Based on this concept, highly floatable material was added directly into the froth zone when treating the anthracite coal. The enriched froth phase reduced the product ash content of the anthracite product by five absolute percentage points while maintaining a constant recovery value.

  9. Kinematic Fitting of Detached Vertices

    Energy Technology Data Exchange (ETDEWEB)

    Mattione, Paul [Rice Univ., Houston, TX (United States)

    2007-05-01

    The eg3 experiment at the Jefferson Lab CLAS detector aims to determine the existence of the $\\Xi_{5}$ pentaquarks and investigate the excited $\\Xi$ states. Specifically, the exotic $\\Xi_{5}^{--}$ pentaquark will be sought by first reconstructing the $\\Xi^{-}$ particle through its weak decays, $\\Xi^{-}\\to\\pi^{-}\\Lambda$ and $\\Lambda\\to\\pi^{-}$. A kinematic fitting routine was developed to reconstruct the detached vertices of these decays, where confidence level cuts on the fits are used to remove background events. Prior to fitting these decays, the exclusive reaction $\\gamma D\\rightarrow pp\\pi^{-}$ was studied in order to correct the track measurements and covariance matrices of the charged particles. The $\\Lambda\\rightarrow p\\pi^{-}$ and $\\Xi^{-}\\to\\pi^{-}\\Lambda$ decays were then investigated to demonstrate that the kinematic fitting routine reconstructs the decaying particles and their detached vertices correctly.

  10. Classification of posterior vitreous detachment

    OpenAIRE

    Kakehashi A; Takezawa M; Akiba J

    2013-01-01

    Akihiro Kakehashi,1 Mikiko Takezawa,1 Jun Akiba21Department of Ophthalmology, Jichi Medical University, Saitama Medical Center, Saitama, 2Kanjodori Eye Clinic, Asahikawa, JapanAbstract: Diagnosing a posterior vitreous detachment (PVD) is important for predicting the prognosis and determining the indication for vitreoretinal surgery in many vitreoretinal diseases. This article presents both classifications of a PVD by slit-lamp biomicroscopy and of a shallow PVD by optical coherence tomography...

  11. Bacterial diversity in five Icelandic geothermal waters: temperature and sinter growth rate effects.

    Science.gov (United States)

    Tobler, Dominique J; Benning, Liane G

    2011-07-01

    The microbial ecology associated with siliceous sinters was studied in five geochemically diverse Icelandic geothermal systems. Bacterial 16S rRNA clone libraries were constructed from water-saturated precipitates from each site resulting in a total of 342 bacterial clone sequences and 43 species level phylotypes. In near-neutral, saline (2.6-4.7% salinity) geothermal waters where sinter growth varied between 10 and ~300 kg year(-1) m(-2), 16S rRNA gene analyses revealed very low (no OTUs could be detected) to medium (9 OTUs) microbial activity. The most dominant phylotypes found in these waters belong to marine genera of the Proteobacteria. In contrast, in alkaline (pH = 9-10), meteoric geothermal waters with temperature = 66-96°C and geothermal waters; alkaliphilic thermophilic microbial communities with phylotypes related to heterotrophic and autotrophic microorganisms developed in alkaline high temperature waters, whereas halophilic mesophilic communities dominated coastal geothermal waters.

  12. RISK OF MULTIPLE RECURRING RETINAL DETACHMENT AFTER PRIMARY RHEGMATOGENOUS RETINAL DETACHMENT REPAIR.

    Science.gov (United States)

    Enders, Philip; Schick, Tina; Schaub, Friederike; Kemper, Carolin; Fauser, Sascha

    2017-05-01

    To evaluate functional and anatomical outcomes of patients with retinal redetachments (re-RD) after surgery for primary rhegmatogenous retinal detachment. Medical records of eyes with re-RD after rhegmatogenous retinal detachment surgery between 1999 and 2014 at the Department of Ophthalmology, University of Cologne, Germany, were retrospectively evaluated. Data included preoperative and postoperative clinical findings, best-corrected visual acuity, presence and grade of proliferative vitreoretinopathy, surgical procedures, and complication rates. Three hundred and twenty-eight eyes of 2,457 developed a re-RD (13.3%). Of these 328 eyes, 242 eyes (73.8%) had only one re-RD, whereas 86 eyes (26.2%) had 2 or more re-RDs. Visible presence of proliferative vitreoretinopathy during first redetachment surgery increased risk of re-RD with relative risk ratio of 1.46 (P = 0.05). Best-corrected visual acuity deteriorated with every additional re-RD (P < 0.001). Two hundred and thirty-seven eyes received oil endotamponde at least once. In 91 cases, oil endotamponade was left for long-term until last follow-up. Multiple re-RD (≥2 re-RDs) is an infrequent complication after rhegmatogenous retinal detachment surgery. After a first re-RD occurred, risk for multiple re-RD doubles compared with the risk of a first redetachment. Mean functional outcome is unfavorable, whereas predictability remains nevertheless poor because of the wide range of interindividual postoperative best-corrected visual acuity.

  13. Surgical and Visual Outcome for Recurrent Retinal Detachment Surgery

    Directory of Open Access Journals (Sweden)

    Constantin Pournaras

    2014-01-01

    Full Text Available Purpose. To evaluate the anatomical and functional outcome of repeated surgeries for recurrent retinal detachment. Methods. We retrospectively reviewed 70 cases with refractory retinal detachment of various etiologies that required multiple operations. Anatomical success (attached retina or failure (totally/partially-detached retina was assessed biomicroscopically. The BCVA was used for the evaluation of the functional outcome, at presentation and at the end of follow-up. Various pre-, intra-, and postoperative factors were associated with anatomical success or failure as well as with final functionality. Results. The mean number of surgeries was 4 (range: 2 to 10. The anatomical success rate was 80% (56 attached cases, 14 detached cases. 29% of the attached cases had a BCVA better than 20/40 (Snellen chart. The number of operations doesn’t seem to affect significantly the final visual acuity. The PVR was found to affect both the anatomical and functional outcome (P=0.014 & P=0.002, respectively. Conclusions. In the present study, it is suggested that multiple operations for refractory retinal detachment may result in successful anatomic results, with a fare functional outcome at the same time. Eventually, we verified that the existence of PVR worsens the prognosis.

  14. Repeat Descemetopexy after Descemet's Membrane Detachment following Phacoemulsification

    Directory of Open Access Journals (Sweden)

    Sameer Datar

    2014-07-01

    Full Text Available Descemet's membrane detachment (DMD is an uncommon condition with a wide range of possible etiologies. Probably the commonest cause is a localized detachment occurring after cataract extraction surgery. Descemetopexy gives good anatomic attachment rates and visual outcomes and has become the standard treatment for DMD. However, in cases with failed initial descemetopexy, the next step in the management of such cases remains unclear. Before initiating a complex surgical procedure like keratoplasty, which requires good postoperative care and regular follow-ups, repeat descemetopexy with a long-term tamponade using 14% C3F8 gas for recurrent DMD is definitely a worthwhile attempt.

  15. [Treatment of primary retinal detachment. Minimal extraocular or intraocular?].

    Science.gov (United States)

    Kreissig, I

    2002-06-01

    The developments in treatment modalities for a primary retinal detachment over the last 70 years have been reviewed. There was a change from a surgery limited to the area of the break to a form of prophylactic surgery including the extent of the detachment. In between Rosengren had limited the treatment to the break with an intraocular gas bubble. A change was brought about by Custodis in 1953 who limited surgery to the break and omitted drainage. This procedure had serious postoperative complications which were eliminated by Lincoff by developing the cryosurgical detachment operation which was subsequently refined to extraocular minimal surgery. The ultimate realization of a minimal extraocular approach was the operation with a temporary balloon. Two additional intraocular procedures evolved, pneumatic retinopexy and primary vitrectomy, following one or the other pattern of treatment. With all four methods reattachment can result in 94-99% of the cases but differences can be seen in the morbidity and rate of reoperations.

  16. Progression of foveola-on rhegmatogenous retinal detachment

    DEFF Research Database (Denmark)

    Hajari, Javad Nouri; Kyhnel, Alexander; Bech-Azeddine, Julia

    2014-01-01

    AIMS: Quantitative assessment of the rate of progression of recent onset posterior rhegmatogenous retinal detachment (RRD). METHODS: A prospective observational study on patients presenting with acute symptomatic primary foveola-on RRD over a 32-month period. Patients were evaluated with optical ...

  17. Classification of posterior vitreous detachment

    Directory of Open Access Journals (Sweden)

    Kakehashi A

    2013-12-01

    Full Text Available Akihiro Kakehashi,1 Mikiko Takezawa,1 Jun Akiba21Department of Ophthalmology, Jichi Medical University, Saitama Medical Center, Saitama, 2Kanjodori Eye Clinic, Asahikawa, JapanAbstract: Diagnosing a posterior vitreous detachment (PVD is important for predicting the prognosis and determining the indication for vitreoretinal surgery in many vitreoretinal diseases. This article presents both classifications of a PVD by slit-lamp biomicroscopy and of a shallow PVD by optical coherence tomography (OCT. By biomicroscopy, the vitreous condition is determined based on the presence or absence of a PVD. The PVD then is classified as either a complete posterior vitreous detachment (C-PVD or a partial posterior vitreous detachment (P-PVD. A C-PVD is further divided into a C-PVD with collapse and a C-PVD without collapse, while a P-PVD is divided into a P-PVD with shrinkage of the posterior hyaloid membrane (P-PVD with shrinkage and a P-PVD without shrinkage of the posterior hyaloid membrane (P-PVD without shrinkage. A P-PVD without shrinkage has a subtype characterized by vitreous gel attachment through the premacular hole in a posterior hyaloid membrane to the macula (P-PVD without shrinkage [M]. By OCT, a shallow PVD is classified as the absence of a shallow PVD or as a shallow PVD. A shallow PVD is then subclassified as a shallow PVD without shrinkage of the posterior vitreous cortex, a shallow PVD with shrinkage of the posterior vitreous cortex, and a peripheral shallow PVD. A shallow PVD without shrinkage of the posterior vitreous cortex has two subtypes: an age-related shallow PVD and a perifoveal PVD associated with a macular hole.Keywords: classification, optical coherence tomography, PVD, slit-lamp biomicroscopy

  18. Classification of posterior vitreous detachment.

    Science.gov (United States)

    Kakehashi, Akihiro; Takezawa, Mikiko; Akiba, Jun

    2014-01-01

    Diagnosing a posterior vitreous detachment (PVD) is important for predicting the prognosis and determining the indication for vitreoretinal surgery in many vitreoretinal diseases. This article presents both classifications of a PVD by slit-lamp biomicroscopy and of a shallow PVD by optical coherence tomography (OCT). By biomicroscopy, the vitreous condition is determined based on the presence or absence of a PVD. The PVD then is classified as either a complete posterior vitreous detachment (C-PVD) or a partial posterior vitreous detachment (P-PVD). A C-PVD is further divided into a C-PVD with collapse and a C-PVD without collapse, while a P-PVD is divided into a P-PVD with shrinkage of the posterior hyaloid membrane (P-PVD with shrinkage) and a P-PVD without shrinkage of the posterior hyaloid membrane (P-PVD without shrinkage). A P-PVD without shrinkage has a subtype characterized by vitreous gel attachment through the premacular hole in a posterior hyaloid membrane to the macula (P-PVD without shrinkage [M]). By OCT, a shallow PVD is classified as the absence of a shallow PVD or as a shallow PVD. A shallow PVD is then subclassified as a shallow PVD without shrinkage of the posterior vitreous cortex, a shallow PVD with shrinkage of the posterior vitreous cortex, and a peripheral shallow PVD. A shallow PVD without shrinkage of the posterior vitreous cortex has two subtypes: an age-related shallow PVD and a perifoveal PVD associated with a macular hole.

  19. Biofilm formation on polystyrene in detached vs. planktonic cells of polyhydroxyalkanoate-accumulating Halomonas venusta.

    Science.gov (United States)

    Berlanga, Mercedes; Domènech, Òscar; Guerrero, Ricardo

    2014-12-01

    Biofilm development is characterized by distinct stages of initial attachment, microcolony formation and maturation (sessile cells), and final detachment (dispersal of new, planktonic cells). In this work we examined the influence of polyhydroxyalkanoate (PHA) accumulation on bacterial surface properties and biofilm formation on polystyrene in detached vs. planktonic cells of an environmental strain isolated from microbial mats, Halomonas venusta MAT28. This strain was cultured either in an artificial biofilm in which the cells were immobilized on alginate beads (sessile) or as free-swimming (planktonic) cells. For the two modes of growth, conditions allowing or preventing PHA accumulation were established. Cells detached from alginate beads and their planktonic counterparts were used to study cell surface properties and cellular adhesion on polystyrene. Detached cells showed a slightly higher affinity than planktonic cells for chloroform (Lewis-acid) and a greater hydrophobicity (affinity for hexadecane and hexane). Those surface characteristics of the detached cells may explain their better adhesion on polystyrene compared to planktonic cells. Adhesion to polystyrene was not significantly different between H. venusta cells that had accumulated PHA vs. those that did not. These observations suggest that the surface properties of detached cells clearly differ from those of planktonic cells and that for at least the first 48 h after detachment from alginate beads H. venusta retained the capacity of sessile cells to adhere to polystyrene and to form a biofilm.

  20. PIC Simulation of plasma detachment

    Science.gov (United States)

    Ishiguro, Seiji; Pianpanit, Theerasarn; Hasegawa, Hiroki; Kanno, Ryutaro

    2014-10-01

    The detached plasma, which is caused by gas puffing, has been proposed and it is the most promising way to reduce the heat load to the divertor plate of fusion oriented devices. Dynamical and kinetic behavior of the detached plasma is unresolved. So we are developing particle-in-cell simulation model with atomic processes such as line radiation, ionization, charge-exchange collision and recombination. As a first step, we have performed PIC simulation with Monte Carlo collisions, where spatial and velocity space distributions of charged particles, self-consistent electric field, and atomic processes such as ionization and charge exchange are included. Temperature decrease and density increase in front of the target is observed and electric potential structure along the axis is created. This work is performed with the support and under the auspices of NIFS Collaboration Research programs (NIFS14KNXN279 and 8 NIFS13KNSS038) and the Research Cooperation Program on Hierarchy and Holism in Natural Sciences at the NINS.

  1. Shock detachment process on cones in hypervelocity flows

    Science.gov (United States)

    Leyva, Ivett A.

    1999-11-01

    The shock detachment process on cones in hypervelocity flows is one of the most sensitive flows to relaxation effects. The critical angle for shock detachment under frozen conditions can be very different from the critical angle under chemical and thermal equilibrium. The rate of increase of the detachment distance with cone angle is also affected by the relaxation rate. The purpose of this study is to explain the effects of nonequilibrium on the shock detachment distance and its growth rate on cones in hypervelocity flows. The study consists of an experimental and a computational program. The experimental part has been carried out at Caltech's hypervelocity reflected shock tunnel (T5). Six different free-stream conditions have been chosen, four using N2 as the test gas and two using CO2. About 170 shots were performed on 24 cones. The cones range in diameter from 2 cm to 16 cm with half-angles varying from 55° to 75°. The experimental data obtained are holographic interferograms of every shot, and surface temperature and pressure measurements for the bigger cones. Extensive numerical simulations were made for the N2 flows and some were also made for the CO2 flows. The code employed is a Navier-Stokes solver that can account for thermal and chemical nonequilibrium in axisymmetric flows. The experimental and computational data obtained for the shock detachment distance confirms a previous theoretical model that predicts the detachment distance will grow more slowly for relaxing flows than for frozen or equilibrium flows. This difference is explained in terms of the behavior of the sonic line inside the shock layer. Different growth rates result when the detachment distance is controlled by the diameter of the cone (frozen and equilibrium cases) than when it is controlled by the extent of the relaxation zone inside the shock layer (nonequilibrium flows). The experimental data are also complemented with computational data to observe the behavior of the detachment

  2. DETACHMENT OF BACTERIOPHAGE FROM ITS CARRIER PARTICLES.

    Science.gov (United States)

    Hetler, D M; Bronfenbrenner, J

    1931-05-20

    The active substance (phage) present in the lytic broth filtrate is distributed through the medium in the form of particles. These particles vary in size within broad limits. The average size of these particles as calculated on the basis of the rate of diffusion approximates 4.4 mmicro in radius. Fractionation by means of ultrafiltration permits partial separation of particles of different sizes. Under conditions of experiments here reported the particles varied in the radius size from 0.6 mmicro to 11.4 mmicro. The active agent apparently is not intimately identified with these particles. It is merely carried by them by adsorption, and under suitable experimental conditions it can be detached from the larger particles and redistributed on smaller particles of the medium.

  3. Anthocyanin Incorporated Dental Copolymer: Bacterial Growth Inhibition, Mechanical Properties, and Compound Release Rates and Stability by 1H NMR

    Directory of Open Access Journals (Sweden)

    Halyna Hrynash

    2014-01-01

    Full Text Available Objective. To evaluate bacterial growth inhibition, mechanical properties, and compound release rate and stability of copolymers incorporated with anthocyanin (ACY; Vaccinium macrocarpon. Methods. Resin samples were prepared (Bis-GMA/TEGDMA at 70/30 mol% and incorporated with 2 w/w% of either ACY or chlorhexidine (CHX, except for the control group. Samples were individually immersed in a bacterial culture (Streptococcus mutans for 24 h. Cell viability (n=3 was assessed by counting the number of colony forming units on replica agar plates. Flexural strength (FS and elastic modulus (E were tested on a universal testing machine (n=8. Compound release and chemical stability were evaluated by UV spectrophotometry and 1H NMR (n=3. Data were analyzed by one-way ANOVA and Tukey’s test (α = 0.05. Results. Both compounds inhibited S. mutans growth, with CHX being most effective (P<0.05. Control resin had the lowest FS and E values, followed by ACY and CHX, with statistical difference between control and CHX groups for both mechanical properties (P<0.05. The 24 h compound release rates were ACY: 1.33 μg/mL and CHX: 1.92 μg/mL. 1H NMR spectra suggests that both compounds remained stable after being released in water. Conclusion. The present findings indicate that anthocyanins might be used as a natural antibacterial agent in resin based materials.

  4. Roller coaster-associated retinal detachments.

    Science.gov (United States)

    Shaikh, Saad

    2011-01-01

    The purpose of this study was to report two cases of rhegmatogenous retinal detachment noted immediately after roller coaster riding in an at-risk population. In separate incidents, a 35-year-old woman and a 45-year-old woman, both significantly myopic, presented with visual symptoms after riding roller coasters. Both patients were found to have acute rhegmatogenous retinal detachments associated with myopic degenerative changes. The pathology supported an acute, traumatic etiology for the detachments. Roller coaster riding should be considered an adjunct risk factor for retinal detachment in predisposed patients.

  5. Vitrectomy, lensectomy and silicone oil tamponade in the management of retinal detachment associated with choroidal detachment

    Directory of Open Access Journals (Sweden)

    Jian-Di Liu

    2013-06-01

    Full Text Available AIM: To report the results of combined vitrectomy, lensectomy and silicone oil (SO tamponade in treating primary rhegmatogenous retinal detachment (RRD associated with choroidal detachment (CD.METHODS: A retrospective, consecutive and case series study of 21 subjects with concurrent RRD associated with CD was conducted. All subjects underwent a standard three-port 20G pars plana vitrectomy (PPV with lensectomy and silicone oil tamponade. Mean follow-up time was 8 months (rang from 4 to 19 months. The primary and final anatomic success rate, visual acuity and final intraocular pressure(IOP were recorded and analyzed.RESULTS: Of 21 subjects, 8 were women and 13 were men. Age at presentation ranged from 22 to 75 years (mean 57.4 years. The presenting vision ranged from light perception to 0.15. The initial IOP ranged from 3mmHg to 12mmHg (mean 6.2mmHg. All eyes were phakic except one pseudophakic. No intraocular lens was implanted during the primary surgical intervention. Fifteen of 21 (71.4% eyes had retina reattached after one operation. Six eyes had recurrent inferior retinal detachment due to proliferation. Five of them were successfully reattached after one or more additional operations. Mean IOP at final follow-up was 15.2mmHg (range from 8mmHg to 20mmHg. One case declined for further operation. The final reattachment rate was 95.2%. Visual acuity improved in 19 (90.5% eyes, was unchanged in 1 (4.8% eye and decreased in 1 (4.8% eye.CONCLUSION: Combination of vitrectomy, lensectomy and silicone tamponade is an effective method in treating RRD associated with CD, reducing the incidence of postoperative hypotony.

  6. Determinants of the rate of mRNA translocation in bacterial protein synthesis.

    Science.gov (United States)

    Borg, Anneli; Ehrenberg, Måns

    2015-05-08

    Studying the kinetics of translocation of mRNA and tRNAs on the translating ribosome is technically difficult since the rate-limiting steps involve large conformational changes without covalent bond formation or disruption. Here, we have developed a unique assay system for precise estimation of the full translocation cycle time at any position in any type of open reading frame (ORF). Using a buffer system optimized for high accuracy of tRNA selection together with high concentration of elongation factor G, we obtained in vivo compatible translocation rates. We found that translocation was comparatively slow early in the ORF and faster further downstream of the initiation codon. The maximal translocation rate decreased from the in vivo compatible value of 30 s(-1) at 1 mM free Mg2+ concentration to the detrimentally low value of 1 s(-1) at 6 mM free Mg2+ concentration. Thus, high and in vivo compatible accuracy of codon translation, as well as high and in vivo compatible translocation rate, required a remarkably low Mg2+ concentration. Finally, we found that the rate of translocation deep inside an ORF was not significantly affected upon variation of the standard free energy of interaction between a 6-nt upstream Shine-Dalgarno (SD)-like sequence and the anti-SD sequence of 16S rRNA in a range of 0-6 kcal/mol. Based on these experiments, we discuss the optimal choice of Mg2+ concentration for maximal fitness of the living cell by taking its effects on the accuracy of translation, the peptide bond formation rate and the translocation rate into account.

  7. Growth against entropy in bacterial metabolism: the phenotypic trade-off behind empirical growth rate distributions in E. coli

    Science.gov (United States)

    De Martino, Daniele; Capuani, Fabrizio; De Martino, Andrea

    2016-06-01

    The solution space of genome-scale models of cellular metabolism provides a map between physically viable flux configurations and cellular metabolic phenotypes described, at the most basic level, by the corresponding growth rates. By sampling the solution space of E. coli's metabolic network, we show that empirical growth rate distributions recently obtained in experiments at single-cell resolution can be explained in terms of a trade-off between the higher fitness of fast-growing phenotypes and the higher entropy of slow-growing ones. Based on this, we propose a minimal model for the evolution of a large bacterial population that captures this trade-off. The scaling relationships observed in experiments encode, in such frameworks, for the same distance from the maximum achievable growth rate, the same degree of growth rate maximization, and/or the same rate of phenotypic change. Being grounded on genome-scale metabolic network reconstructions, these results allow for multiple implications and extensions in spite of the underlying conceptual simplicity.

  8. Detached divertor plasmas in JET

    Energy Technology Data Exchange (ETDEWEB)

    Horton, L.D.; Borrass, K.; Corrigan, G.; Gottardi, N.; Lingertat, J.; Loarte, A.; Simonini, R.; Stamp, M.F.; Taroni, A. [Commission of the European Communities, Abingdon (United Kingdom). JET Joint Undertaking; Stangeby, P.C. [Toronto Univ., ON (Canada). Inst. for Aerospace Studies

    1994-07-01

    In simulations with high radiated power fractions, it is possible to produce the drop in ion current to the divertor targets typical of detached plasmas. Despite the fact that these experiments are performed on beryllium target tiles, radiation from deuterium and beryllium cannot account for the measured power losses. The neutral deuterium levels in the SOL in these plasmas are higher than the model predicts. This may be due to leakage from the divertor or to additional wall sources related to the non-steady nature of these plasmas. In contrast, a surprisingly high level of carbon is present in these discharges; higher even than would be predicted are the divertor target tiles pure carbon. This level may well be large enough to produce the measured radiation. (authors). 6 refs., 2 figs., 1 tab.

  9. Bacterial growth on surfaces: Automated image analysis for quantification of growth rate-related parameters

    DEFF Research Database (Denmark)

    Møller, S.; Sternberg, Claus; Poulsen, L. K.

    1995-01-01

    species-specific hybridizations with fluorescence-labelled ribosomal probes to estimate the single-cell concentration of RNA. By automated analysis of digitized images of stained cells, we determined four independent growth rate-related parameters: cellular RNA and DNA contents, cell volume......, and the frequency of dividing cells in a cell population. These parameters were used to compare physiological states of liquid-suspended and surfacegrowing Pseudomonas putida KT2442 in chemostat cultures. The major finding is that the correlation between substrate availability and cellular growth rate found...

  10. Influence of biogas flow rate on biomass composition during the optimization of biogas upgrading in microalgal-bacterial processes.

    Science.gov (United States)

    Serejo, Mayara L; Posadas, Esther; Boncz, Marc A; Blanco, Saúl; García-Encina, Pedro; Muñoz, Raúl

    2015-03-03

    The influence of biogas flow rate (0, 0.3, 0.6, and 1.2 m(3) m(-2) h(-1)) on the elemental and macromolecular composition of the algal-bacterial biomass produced from biogas upgrading in a 180 L photobioreactor interconnected to a 2.5 L external bubbled absorption column was investigated using diluted anaerobically digested vinasse as cultivation medium. The influence of the external liquid recirculation/biogas ratio (0.5 biogas, was also evaluated. A L/G ratio of 10 was considered optimum to support CO2 and H2S removals of 80% and 100%, respectively, at all biogas flow rates tested. Biomass productivity increased at increasing biogas flow rate, with a maximum of 12 ± 1 g m(-2) d(-1) at 1.2 m(3) m(-2) h(-1), while the C, N, and P biomass content remained constant at 49 ± 2%, 9 ± 0%, and 1 ± 0%, respectively, over the 175 days of experimentation. The high carbohydrate contents (60-76%), inversely correlated to biogas flow rates, would allow the production of ≈100 L of ethanol per 1000 m(3) of biogas upgraded under a biorefinery process approach.

  11. Influence of body temperature on bacterial growth rates in experimental pneumococcal meningitis in rabbits.

    OpenAIRE

    Small, P M; Täuber, M G; Hackbarth, C J; Sande, M A

    1986-01-01

    We examined the role of fever as a host defense in experimental pneumococcal meningitis in rabbits. Twelve hours after intracisternal inoculation of an encapsulated type 3 Streptococcus pneumoniae strain, body temperature was manipulated by using two different anesthetic drugs: pentobarbital, which did not affect temperature, and urethane, which mitigated the febrile response to infection. Growth rates of pneumococci in cerebrospinal fluid were dramatically influenced by modification of the f...

  12. Turbulent Simulations of Divertor Detachment Based On BOUT + + Framework

    Science.gov (United States)

    Chen, Bin; Xu, Xueqiao; Xia, Tianyang; Ye, Minyou

    2015-11-01

    China Fusion Engineering Testing Reactor is under conceptual design, acting as a bridge between ITER and DEMO. The detached divertor operation offers great promise for a reduction of heat flux onto divertor target plates for acceptable erosion. Therefore, a density scan is performed via an increase of D2 gas puffing rates in the range of 0 . 0 ~ 5 . 0 ×1023s-1 by using the B2-Eirene/SOLPS 5.0 code package to study the heat flux control and impurity screening property. As the density increases, it shows a gradually change of the divertor operation status, from low-recycling regime to high-recycling regime and finally to detachment. Significant radiation loss inside the confined plasma in the divertor region during detachment leads to strong parallel density and temperature gradients. Based on the SOLPS simulations, BOUT + + simulations will be presented to investigate the stability and turbulent transport under divertor plasma detachment, particularly the strong parallel gradient driven instabilities and enhanced plasma turbulence to spread heat flux over larger surface areas. The correlation between outer mid-plane and divertor turbulence and the related transport will be analyzed. Prepared by LLNL under Contract DE-AC52-07NA27344. LLNL-ABS-675075.

  13. Quantum wavepacket study of electron detachment from ? by electron impact

    Science.gov (United States)

    Kazansky, A. K.; Taulbjerg, K.

    1996-10-01

    Electron detachment from negative hydrogen ions by low-energy electron impact is considered as an especially simple example to test the validity of a sequence of approximations that allow quantum wavepacket computations to be implemented with desktop facilities. In the present approach, the motion of the electron initially localized in the loosely bound ion state is treated quantum mechanically while the incident electron is assumed to move along a classical trajectory. In addition, the initial azimuthal symmetry is assumed to be conserved during the collision in the co-rotating frame such that the quantum motion is restricted to two degrees of freedom. The wave-dynamical approach is used directly to determine the electron detachment cross section. It also provides a valuable means to clarify physical aspects of the considered process. As examples, we show that the effective detachment rate is strongly affected by projectile-induced polarization effects and that non-adiabatic features are important for a complete account of the electron detachment process. The results of the wave-dynamic calculations are in excellent agreement with recent experimental data.

  14. Production rates of bacterial tetraether lipids and fatty acids in peatland under varying oxygen concentrations

    Science.gov (United States)

    Huguet, Arnaud; Meador, Travis B.; Laggoun-Défarge, Fatima; Könneke, Martin; Wu, Weichao; Derenne, Sylvie; Hinrichs, Kai-Uwe

    2017-04-01

    Interpretations of the abundance and distribution of branched glycerol dialkyl glycerol tetraether (brGDGT) lipids have been increasingly applied to infer changes in paleoenvironment and to estimate terrigenous organic matter inputs into estuarine and marine sediments. However, only preliminary information is known regarding the ecology and physiology of the source organisms of these biomarkers. We assessed the production rates of brGDGTs under different redox conditions in peat, where these lipids are found in high concentrations, particularly at greater depths below the fluctuating water table. The incorporation of hydrogen relative to carbon into lipids observed in our dual stable isotope probing assay indicates that brGDGTs were produced by heterotrophic bacteria. Unexpectedly, incubations with stable isotope tracers of the surface horizon (5-20 cm) initiated under oxic conditions before turning suboxic and eventually anoxic exhibited up to one order of magnitude higher rates of brGDGT production (16-87 ng cm-3 y-1) relative to the deeper, anoxic zone (20-35 cm; ca. 7 ng cm-3 y-1), and anoxic incubations of the surface horizon (cell membrane in comparison to fatty acids, despite the typically high brGDGT concentrations observed in peat. Multivariate analysis identified two branched fatty acids that shared a similar production pattern as brGDGTs among the experimental treatments and may be associated with brGDGT biosynthesis.

  15. Chemoautotrophic carbon fixation rates and active bacterial communities in intertidal marine sediments.

    Directory of Open Access Journals (Sweden)

    Henricus T S Boschker

    Full Text Available Chemoautotrophy has been little studied in typical coastal marine sediments, but may be an important component of carbon recycling as intense anaerobic mineralization processes in these sediments lead to accumulation of high amounts of reduced compounds, such as sulfides and ammonium. We studied chemoautotrophy by measuring dark-fixation of 13C-bicarbonate into phospholipid derived fatty acid (PLFA biomarkers at two coastal sediment sites with contrasting sulfur chemistry in the Eastern Scheldt estuary, The Netherlands. At one site where free sulfide accumulated in the pore water right to the top of the sediment, PLFA labeling was restricted to compounds typically found in sulfur and ammonium oxidizing bacteria. At the other site, with no detectable free sulfide in the pore water, a very different PLFA labeling pattern was found with high amounts of label in branched i- and a-PLFA besides the typical compounds for sulfur and ammonium oxidizing bacteria. This suggests that other types of chemoautotrophic bacteria were also active, most likely Deltaproteobacteria related to sulfate reducers. Maximum rates of chemoautotrophy were detected in first 1 to 2 centimeters of both sediments and chemosynthetic biomass production was high ranging from 3 to 36 mmol C m(-2 d(-1. Average dark carbon fixation to sediment oxygen uptake ratios were 0.22±0.07 mol C (mol O2(-1, which is in the range of the maximum growth yields reported for sulfur oxidizing bacteria indicating highly efficient growth. Chemoautotrophic biomass production was similar to carbon mineralization rates in the top of the free sulfide site, suggesting that chemoautotrophic bacteria could play a crucial role in the microbial food web and labeling in eukaryotic poly-unsaturated PLFA was indeed detectable. Our study shows that dark carbon fixation by chemoautotrophic bacteria is a major process in the carbon cycle of coastal sediments, and should therefore receive more attention in future

  16. Retinal detachment after open globe injury.

    Science.gov (United States)

    Stryjewski, Tomasz P; Andreoli, Christopher M; Eliott, Dean

    2014-01-01

    To characterize the development of retinal detachment (RD) after open globe trauma. Case-control study. A total of 892 patients comprising 893 open globe injuries (OGIs), of whom 255 were ultimately diagnosed with RD, with the remaining eyes serving as controls. Retrospective chart review of patients with OGIs presenting to the Massachusetts Eye and Ear Infirmary between 1999 and 2011. Kaplan-Meier analysis was used to estimate the time to detachment, and multivariable logistic regression was used to define the clinical factors associated with RD after OGI. Demographic and clinical characteristics at the time of presentation after OGI, date of RD diagnosis, and last date of follow-up. Primary repair of the open globe was typically undertaken within hours of presentation. A total of 255 eyes were ultimately diagnosed with RD after open globe trauma, yielding an incidence of 29% (95% confidence interval, 26-32). For eyes that developed RD, 27% (69/255) detached within 24 hours of primary open globe repair, 47% (119/255) detached within 1 week, and 72% (183/255) detached within 1 month. Multivariable regression analysis revealed the presence of vitreous hemorrhage (odds ratio [OR], 7.29; P Globe Injury score. Retinal detachment is common after open globe trauma, although often not appearing until days to weeks after the initial traumatic event. Several clinical variables at the time of initial presentation can predict the future risk of detachment. Copyright © 2014 American Academy of Ophthalmology. Published by Elsevier Inc. All rights reserved.

  17. Curative effect of minimally invasive sclera buckling on single retinal detachment

    Directory of Open Access Journals (Sweden)

    Yun-Huan Li

    2015-02-01

    Full Text Available AIM: To investigate the curative effect of minimally invasive sclera buckling on single retinal detachment. METHODS:Totally, 100 cases of patients with retinal detachment(106 eyesenrolled in our hospital were randomly divided into observation group and control group, 53 eyes in each group. Patients in observation group were treated with minimally invasive sclera buckling, while patients in control group received traditional limbal conjunctival incision. After surgery, patients were all followed up for 6~18mo, during which the retinal recurrence situation, degree of vision enhancement and compliance occurrence rate was recorded. RESULTS: The retinal reattachment rate once of observation group(96.22%was significantly higher than that of control group(88.68%, there was statistically significance(PPPCONCLUSION: The improved minimally invasive sclera buckling can significantly enhance the curative effect for retinal detachment, decrease the compliance occurrence rate, improve vision function, and is a scientific, practical and rigorous tool for retinal detachment treatment.

  18. The effect of slope angle on splash detachment in steep forest plantation

    Science.gov (United States)

    Mizugaki, S.; Nanko, K.; Onda, Y.

    2007-12-01

    To study splash detachment rate and investigate the effects of rainfall and slope angle on splash detachment, the field observation of splash detachment was conducted for five months using 27 splash cups under natural rainfall events in Japanese cypress (Hinoki; Chamaecyparis obtusa) plantation in the Shimanto River watershed, southern Japan. In this plantation forest, the unit kinetic energy of throughfall (unit KE; J/m2/mm) was found to be constant independent of rainfall intensity. The total rainfall over six observation periods was 853 mm and the maximum rainfall intensity for 1 h ( RI1h) varied from 8.0 to 19.6 mm h-1. A significantly high coefficient of linear regression was found between RI1h and the average splash detachment of all splash cups over six periods, although the splash detachment from the individual cups had larger variations with RI1h. This variation in splash detachment may attribute to the spatial variability in soil surface condition such as slope angle. In the relationship between the splash detachment and slope angle, no correlation was found over the entire periods. However, different correlations were found among the observation periods due to the differences in rainfall intensity. The splash detachment from a lower slope angle (14°) exhibited a strong relation with the maximum rainfall intensity for a shorter period, such as 10 to 30 minutes. In contrast, the splash detachment from a slope angle of over 35° exhibited high correlation with the maximum rainfall intensity for 3 h, suggesting that longer time is required for ponding in steeper slopes than gentler slopes. In gentler slopes, prolonged rainfall may cause the higher ponding depth, resulting in reducing the raindrop impact and less splash detachment. Therefore, under the forest canopies, the effect of slope angle on the rainfall parameter should be incorporated into the future splash erosion model.

  19. Bubble Growth and Detachment from a Needle

    Science.gov (United States)

    Shusser, Michael; Rambod, Edmond; Gharib, Morteza

    1999-11-01

    The release of bubbles from an underwater nozzle or orifice occurs in large number of applications, such as perforated plate columns, blood oxygenators and various methods of water treatment. It is also a widely used method in laboratory research on multiphase flow and acoustics for generating small bubbles in a controlled fashion. We studied experimentally the growth and pinch-off of air bubbles released from a submerged needle into a quiescent liquid or a liquid flowing parallel to the needle. Micron-sized bubbles were generated by an air-liquid dispenser. High-speed imaging was performed to study the formation and detachment of bubbles from the tip of the needle. The impact of the needle diameter was investigated and the size and number of produced bubbles were assessed for different flow rates of air and for different velocities of the imposed upward liquid flow. The results were compared with available theoretical models and numerical computations. The existence of a critical gas flow rate and two regimes of bubble growth were verified.

  20. Biofilm detachment mechanisms in a liquid-fluidized bed.

    Science.gov (United States)

    Chang, H T; Rittmann, B E; Amar, D; Heim, R; Ehlinger, O; Lesty, Y

    1991-08-20

    Bed fluidization offers the possibility of gaining the advantages of fixed-film biological processes without the disadvantage of pore clogging. However, the biofilm detachment rate, due to hydrodynamics and particle-to-particle attrition, is very poorly understood for fluidized-bed biofilm processes. In this work, a two-phase fluidized-bed biofilm was operated under a constant surface loading (0.09 mg total organic carbon/cm(2) day) and with a range of bed height (H), fluid velocities (U), and support-particle concentrations (C(p)). Direct measurements were made for the specific biofilm loss rate coefficient (b(s))and the total biofilm accumulation (X(f)L(f)). A hydrodynamic model allowed independent determination of the biofilm density (X(f)), biofilm thickness (L(f)), liquid shear stress (tau), and Reynolds number (Re). Multiple regression analysis of the results showed that increased particle-to-particle attrition, proportional to C(p) and increased turbulence, described by Re, caused the biofilms to be denser and thinner. The specific detachment rate coefficient (b(s)) increased as C(p) and Re increased. Almost all of the 6, values were larger than predicted by a previous model derived for smooth biofilms on a nonfluidized surface. Therefore, the turbulence and attrition of bed fluidization appear to be dominant detachment mechanisms.

  1. Timing and Outcomes of Vitreoretinal Surgery after Traumatic Retinal Detachment

    Directory of Open Access Journals (Sweden)

    Molly Orban

    2016-01-01

    Full Text Available Traumatic retinal detachments are a significant cause of morbidity. There are currently no evidence-based guidelines on the appropriate time to perform vitreoretinal surgery to repair a traumatic retinal detachment. Early intervention, within seven days of the inciting trauma, may decrease proliferative vitreoretinopathy and postoperative endophthalmitis. Later intervention may yield a reduced risk of inflammation and hemorrhage, particularly in cases of concomitant open globe injuries. This article reviews the literature on the management of retinal detachments associated with ocular trauma from the years 2006 to 2016. Particular focus was placed on the timing of surgery, concomitant open globe injury, anatomical success rates, visual acuity, and complication rates. In this review, anatomical success was not significantly related to timing of intervention when compared between early and delayed intervention in eyes with and without concomitant open globe injuries. Visual acuities postoperatively varied widely despite timing of intervention due to the large variation in mechanism and extent of ocular injuries. Proliferative vitreoretinopathy was a common complication. Preliminary data indicate that endophthalmitis rates may be lower when early vitreoretinal surgery is performed. There is insufficient data to conclude whether early or delayed surgery leads to improved outcomes, highlighting the need for further research in this domain.

  2. [Intraocular hypertension after retinal detachment surgery].

    Science.gov (United States)

    Muşat, O; Cristescu, R; Coman, Corina; Asandi, R

    2012-01-01

    This papers presents a case of a patient with retinal detachment, 3 days ago operated (posterior vitrectomy, internal tamponament with silicon oil 1000) who developed increased ocular pressure following silicon oil output in the anterior chamber.

  3. [Ocular hypertension after surgery for retinal detachment].

    Science.gov (United States)

    Muşat, O; Cristescu, R; Coman, Corina; Asandi, R

    2012-01-01

    This papers presents a case of a patient with retinal detachement, 3 days ago operated (posterior vitrectomy internal tamponament with silicon oil 1000) who develop increased ocular pressure following silicon oil output in the anterior chamber.

  4. Complications of acute posterior vitreous detachment.

    Science.gov (United States)

    Kanski, J J

    1975-07-01

    Of 201 patients whose presenting symptoms were acute entoptic phenomena or photopsia, or both, 150 patients had posterior vitreous detachment; 69 patients (46%) had retinal breaks; 18 (12%) had a vitreous hemorrhage without detectable retinal breaks; and two (1.3%) had peripheral retinal hemorrhages without retinal breaks or vitreous hemorrhage. Retinal breaks that occur in eyes in conjunction with acute posterior vitreous detachment are potentially dangerous and there is a possibility of delayed break formation.

  5. Current surgery of retinal detachment recurrence. Review

    Directory of Open Access Journals (Sweden)

    V. D. Zakharov

    2012-01-01

    Full Text Available this review presents a detailed analysis and an experience of surgical treatment of retinal detachment recurrence associated with light silicone oil tamponade of vitreous cavity. Approaches and variants of treatment were described in the historical aspect and till now. there are considered general and particular issues in case of retinal detachment recurrence appearance, expediency and volume of intraoperative manipulations, time of operation and choice of temporary substitute of vitreous body for a purpose of postoperative tamponade of vitreous cavity.

  6. Current surgery of retinal detachment recurrence. Review

    Directory of Open Access Journals (Sweden)

    V. D. Zakharov

    2014-07-01

    Full Text Available this review presents a detailed analysis and an experience of surgical treatment of retinal detachment recurrence associated with light silicone oil tamponade of vitreous cavity. Approaches and variants of treatment were described in the historical aspect and till now. there are considered general and particular issues in case of retinal detachment recurrence appearance, expediency and volume of intraoperative manipulations, time of operation and choice of temporary substitute of vitreous body for a purpose of postoperative tamponade of vitreous cavity.

  7. The DEBCat detached eclipsing binary catalogue

    OpenAIRE

    2014-01-01

    Detached eclipsing binary star systems are our primary source of measured physical properties of normal stars. I introduce DEBCat: a catalogue of detached eclipsing binaries with mass and radius measurements to the 2% precision necessary to put useful constraints on theoretical models of stellar evolution. The catalogue was begun in 2006, as an update of the compilation by Andersen (1991). It now contains over 170 systems, and new results are added on appearance in the refereed literature. DE...

  8. Fluoroquinolone-macrolide combination therapy for chronic bacterial prostatitis: retrospective analysis of pathogen eradication rates, inflammatory findings and sexual dysfunction

    Institute of Scientific and Technical Information of China (English)

    Vittorio Magri; Emanuele Montanari; Vi(s)nja (S)kerk; Alemka Markoti(c); Emanuela Marras; Antonella Restelli; Kurt G Naber; Gianpaolo Perletti

    2011-01-01

    We previously demonstrated the safety and efficacy of fluoroquinolone-macrolide combination therapy in category Ⅱ chronic bacterial prostatitis (CBP).The aim of this study is to retrospectively compare the microbiological and clinical findings of two treatment schemes for CBP based on the combination of azithromycin (500 mg,thrice-weekly) with a once-daily 500-or 750-mg dose of ciprofloxacin (Cipro-500 or Cipro-750 cohort,respectively).Combined administration of azithromycin (1500 mg week-1) with ciprofloxacin at the rate of 750 mg day-1 for 4 weeks rather than at 500 mg day-1 for 6 weeks increased the eradication rates from 62.35% to 77.32% and the total bacteriological success from 71.76% to 85.57%.A significant decrease in pain and voiding signs/symptoms and a significant reduction in inflammatory leukocyte counts and serum prostate-specific antigen (PSA) were sustained throughout an 18-month follow-up period in both groups.Ejaculatory pain,haemospermia and premature ejaculation were significantly attenuated on microbiological eradication in both groups,but the latter subsided more promptly in the Cipro-750 cohort.In total,59 Cipro-750 patients showed mild-to-severn erectile dysfunction (ED) at baseline,while 22 patients had no ED on microbiological eradication and throughout the follow-up period.In conclusion fluoroquinolone-macrolide therapy resulted in pathogen eradication and CBP symptom attenuation,including pain,voiding disturbances and sexual dysfunction.A once-daily 750-mg dose of ciprofloxacin for 4 weeks showed enhanced eradication rates and lower inflammatory white blood cell counts compared to the 500-mg dose for 6 weeks.Our results are open to further prospective validation.

  9. Fluoroquinolone–macrolide combination therapy for chronic bacterial prostatitis: retrospective analysis of pathogen eradication rates, inflammatory findings and sexual dysfunction

    Science.gov (United States)

    Magri, Vittorio; Montanari, Emanuele; Škerk, Višnja; Markotić, Alemka; Marras, Emanuela; Restelli, Antonella; Naber, Kurt G; Perletti, Gianpaolo

    2011-01-01

    We previously demonstrated the safety and efficacy of fluoroquinolone–macrolide combination therapy in category II chronic bacterial prostatitis (CBP). The aim of this study is to retrospectively compare the microbiological and clinical findings of two treatment schemes for CBP based on the combination of azithromycin (500 mg, thrice-weekly) with a once-daily 500- or 750-mg dose of ciprofloxacin (Cipro-500 or Cipro-750 cohort, respectively). Combined administration of azithromycin (1500 mg week−1) with ciprofloxacin at the rate of 750 mg day−1 for 4 weeks rather than at 500 mg day−1 for 6 weeks increased the eradication rates from 62.35% to 77.32% and the total bacteriological success from 71.76% to 85.57%. A significant decrease in pain and voiding signs/symptoms and a significant reduction in inflammatory leukocyte counts and serum prostate-specific antigen (PSA) were sustained throughout an 18-month follow-up period in both groups. Ejaculatory pain, haemospermia and premature ejaculation were significantly attenuated on microbiological eradication in both groups, but the latter subsided more promptly in the Cipro-750 cohort. In total, 59 Cipro-750 patients showed mild-to-severe erectile dysfunction (ED) at baseline, while 22 patients had no ED on microbiological eradication and throughout the follow-up period. In conclusion fluoroquinolone–macrolide therapy resulted in pathogen eradication and CBP symptom attenuation, including pain, voiding disturbances and sexual dysfunction. A once-daily 750-mg dose of ciprofloxacin for 4 weeks showed enhanced eradication rates and lower inflammatory white blood cell counts compared to the 500-mg dose for 6 weeks. Our results are open to further prospective validation. PMID:21765442

  10. Stability of Menisci in Detached Bridgman Growth

    Science.gov (United States)

    Mazuruk, Konstantin; Volz, Martin P.

    2013-01-01

    Detached growth, also referred to as dewetted growth, is a Bridgman crystal growth process in which the melt is in contact with the crucible wall but the crystal is not. A meniscus bridges the gap between the top of the crystal and the crucible wall. The meniscus shape depends on the contact angle of the melt with the crucible wall, the growth angle of the melt with respect to the solidifying crystal, the gas pressure differential, the Weber number describing the rotation rate of the crucible, and the Bond number. Only some of the meniscus shapes are stable and the stability criterion is the sign of the second variation of the potential energy upon admissible meniscus shape perturbations. The effects of confined gas volumes above and below the melt and crucible rotation are evaluated. The analysis is applicable to the non-stationary case where the crystal radius changes during growth. Static stability maps (crystal radius versus pressure differential) are obtained for a series of Bond numbers, growth angles and Weber numbers. Also, the specific cases of Ge and InSb, in both terrestrial and microgravity conditions, are analyzed. Stability was found to depend significantly on whether the interior surface was considered to be microscopically rough or smooth, corresponding to pinned or unpinned states. It was also found that all meniscus shapes are statically stable in a microgravity environment.

  11. Modeling of detachment experiments at DIII-D

    Energy Technology Data Exchange (ETDEWEB)

    Canik, J.M., E-mail: canikjm@ornl.gov [Oak Ridge National Laboratory, PO Box 2008, Oak Ridge, TN 37831 (United States); Briesemeister, A.R. [Oak Ridge National Laboratory, PO Box 2008, Oak Ridge, TN 37831 (United States); Lasnier, C.J. [Lawrence Livermore National Laboratory, 700 East Ave, Livermore, CA 94550 (United States); Leonard, A.W. [General Atomics, PO Box 85608, San Diego, CA 92186-5608 (United States); Lore, J.D. [Oak Ridge National Laboratory, PO Box 2008, Oak Ridge, TN 37831 (United States); McLean, A.G. [Lawrence Livermore National Laboratory, 700 East Ave, Livermore, CA 94550 (United States); Watkins, J.G. [Sandia National Laboratories, 7011 East Ave, Livermore, CA 94550 (United States)

    2015-08-15

    Edge fluid–plasma/kinetic–neutral modeling of well-diagnosed DIII-D experiments is performed in order to document in detail how well certain aspects of experimental measurements are reproduced within the model as the transition to detachment is approached. Results indicate, that at high densities near detachment onset, the poloidal temperature profile produced in the simulations agrees well with that measured in experiment. However, matching the heat flux in the model requires a significant increase in the radiated power compared to what is predicted using standard chemical sputtering rates. These results suggest that the model is adequate to predict the divertor temperature, provided that the discrepancy in radiated power level can be resolved.

  12. Experimental Study of Single and Double Electron Detachment Cross Sections for Cl- in Collision with He

    Institute of Scientific and Technical Information of China (English)

    YANG En-Bo; GAO Mei; HUANG Yong-Yi; WU Shi-Min; LI Guang-Wu; ZHANG Xue-Mei; LU Fu-Quan

    2006-01-01

    @@ Single-electron detachment (SED) and double-electron detachment (DED) for chlorine negative ions in collision with helium are investigated in the energy region of 5-30 keV (SED) and 5-19keV (DED) by growth rate method,respectively. Experimental data from this work are compared with the previous reported data, and a general discussion is given.

  13. Single-Electron Detachment for Ti-,Fe-,Co-,Ni- and Cu- in Collision with Ar

    Institute of Scientific and Technical Information of China (English)

    BAI Xue; ZHAO Jun; WEI Bao-Ren; ZHANG Xue-Mei

    2010-01-01

    @@ By using the micro-channel plate position sensitive detector with the delay-line anode we measure the single electron detachment cross sections for some transition elements in collision with Ar in the energy region 10-30 keV.These single electron detachment cross sections perform as velocity and electron affinity dependences.The experiments are carried out using the growth rate method.

  14. Crust rheology, slab detachment and topography

    Science.gov (United States)

    Duretz, T.; Gerya, T. V.

    2012-04-01

    The collision between continents following the closure of an ocean can lead to the subduction of continental crust. The introduction of buoyant crust within subduction zones triggers the development of extensional stresses in slabs which eventually result in their detachment. The dynamic consequences of slab detachment affects the development of topography, the exhumation of high-pressure rocks and the geodynamic evolution of collision zones. We employ two-dimensional thermo-mechanical modelling in order to study the importance of crustal rheology on the evolution of spontaneous subduction-collision systems and the occurrence of slab detachment. The modelling results indicate that varying the rheological structure of the crust can results in a broad range of collisional evolutions involving slab detachment, delamination (associated to slab rollback), or the combination of both mechanisms. By enhancing mechanical coupling at the Moho, a strong crust leads to the deep subduction of the crust (180 km). These collisions are subjected to slab detachment and subsequent coherent exhumation of the crust accommodated by eduction (inversion of subduction sense) and thrusting. In these conditions, slab detachment promotes the development of a high (> 4.5 km) and narrow (delamination of the lithosphere, preventing slab detachment to occur. Further shortening leads to buckling and thickening of the crust resulting in the development of topographic bulging on the lower plate. Collisions involving rheologically layered crust are characterised by a decoupling level at mid-crustal depths. These initial condition favours the delamination of the upper crust as well as the deep subduction of the lower crust. These collisions are thus successively affected by delamination and slab detachment and both processes contribute to the exhumation of the subducted crust. A wide (> 200 km) topographic plateau develops as the results of the buoyant extrusion of the upper crust onto the foreland

  15. Influence of filtration and glucose amendment on bacterial growth rate at different tidal conditions in the Minho Estuary River (NW Portugal)

    DEFF Research Database (Denmark)

    Anne, I.; Fidalgo, M. L.; Thosthrup, L.

    2006-01-01

    Bacterioplankton abundance, biomass and growth rates were studied in the Minho Estuary River (NW Portugal). The influence of tidal conditions, glucose amendment, and the filtration process on total bacterial abundance, total and faecal coliforms, as well as faecal streptococci, were evaluated...

  16. Gene Transcription Profile of the Detached Retina (An AOS Thesis)

    Science.gov (United States)

    Zacks, David N.

    2009-01-01

    Purpose: Separation of the neurosensory retina from the retinal pigment epithelium (RPE) yields many morphologic and functional consequences, including death of the photoreceptor cells, Müller cell hypertrophy, and inner retinal rewiring. Many of these changes are due to the separation-induced activation of specific genes. In this work, we define the gene transcription profile within the retina as a function of time after detachment. We also define the early activation of kinases that might be responsible for the detachment-induced changes in gene transcription. Methods: Separation of the retina from the RPE was induced in Brown-Norway rats by the injection of 1% hyaluronic acid into the subretinal space. Retinas were harvested at 1, 7, and 28 days after separation. Gene transcription profiles for each time point were determined using the Affymetrix Rat 230A gene microarray chip. Transcription levels in detached retinas were compared to those of nondetached retinas with the BRB-ArrayTools Version 3.6.0 using a random variance analysis of variance (ANOVA) model. Confirmation of the significant transcriptional changes for a subset of the genes was performed using microfluidic quantitative real-time polymerase chain reaction (qRT-PCR) assays. Kinase activation was explored using Western blot analysis to look for early phosphorylation of any of the 3 main families of mitogen-activated protein kinases (MAPK): the p38 family, the Janus kinase family, and the p42/p44 family. Results: Retinas separated from the RPE showed extensive alterations in their gene transcription profile. Many of these changes were initiated as early as 1 day after separation, with significant increases by 7 days. ANOVA analysis defined 144 genes that had significantly altered transcription levels as a function of time after separation when setting a false discovery rate at ≤0.1. Confirmatory RT-PCR was performed on 51 of these 144 genes. Differential transcription detected on the microarray

  17. Role of pulsed winds on detachment of low salinity water from the Pearl River Plume: Upwelling and mixing processes

    Science.gov (United States)

    Chen, Zhaoyun; Pan, Jiayi; Jiang, Yuwu

    2016-04-01

    The detachment of low salinity water (LSW) from the Pearl River plume occurs frequently as revealed by in situ observations and satellite images, and plays an important role in cross-shore transport of the nutrient-rich plume water. In this study, the Regional Ocean Modeling System (ROMS) is used to simulate the LSW detachment process forced by realistic and idealized winds, and to explore its dynamical mechanisms. Modeling results show that the LSW detachment appears under a pulsed southwesterly wind, while tidal mixing modifies the size and salinity of the detached LSW. Strong pulsed wind causes the LSW to separate from the plume and move offshore quickly after the detachment. Under a pulsed northeasterly wind, however, the plume without separation of the LSW moves shoreward, indicating that the LSW detachment is sensitive to wind direction. In the plume region, upwelling develops under the forcing of the pulsed southwesterly wind, which transports high salinity bottom water to the surface layer, while the shear mixing in the upper layer further enhances the surface buoyancy flux, leading to appearance of high salinity water in the surface layer off the Pearl River estuary mouth, cutting off the eastward-spreading plume water, and resulting in the plume LSW detachment. Further analysis shows that the pulsed southwesterly wind induces positive local salinity change rate in the LSW detachment area. The pulsed upwelling-favorable wind with duration of 2-5 days is responsible for the detachment process.

  18. Influence of filtration and glucose amendment on bacterial growth rate at different tidal conditions in the Minho Estuary River (NW Portugal)

    DEFF Research Database (Denmark)

    Anne, I.; Fidalgo, M. L.; Thosthrup, L.;

    2006-01-01

    Bacterioplankton abundance, biomass and growth rates were studied in the Minho Estuary River (NW Portugal). The influence of tidal conditions, glucose amendment, and the filtration process on total bacterial abundance, total and faecal coliforms, as well as faecal streptococci, were evaluated....... In contrast, a significant decrease of bacterial indicators of faecal pollution at high tide was probably the result of various causes, such as the decrease of continental and agriculturalland mn-off effect by dilution, aml/or increase in the abundance of potential specific predators. Thus, drastic changes...

  19. Mobility improvement by detached solidification of CZT crystals

    Energy Technology Data Exchange (ETDEWEB)

    Gamal, G.A. [Physics Department, Faculty of Science, South Valley University, Qena 83523 (Egypt); Abou Zied, M. [Physics Department, Faculty of Science, South Valley University, Qena 83523 (Egypt); Ebnalwaled, A.A. [Physics Department, Faculty of Science, South Valley University, Qena 83523 (Egypt)]. E-mail: Kh_ebnalwaled@yahoo.com

    2007-06-15

    The mobility of undoped CdZnTe (CZT) crystals grown by vertical Bridgman method is improved by growing crystals without wall contact. Among the different factors playing an important role for appearance of detached growth, we have chosen the freezing rate as an effective factor for investigation with other factors were held constant. After growing a set of samples we have measured the dislocation densities for the grown samples by Warren-Averbach method. Unique results were obtained when we compared the dislocation densities and mobility, together with the freezing rate. This work and the included relations are published for the first time.

  20. Retinal Detachment in Down Syndrome: Characteristics and Surgical Outcomes

    Directory of Open Access Journals (Sweden)

    Badr O. AlAhmadi

    2016-01-01

    Full Text Available Purpose. To determine the functional and anatomic outcomes of rhegmatogenous retinal detachment (RRD surgery in patients with Down syndrome. Methods. A retrospective chart review was performed of patients with Down syndrome who had undergone surgery for RRD at King Khalid Eye Specialist Hospital between 1995 and 2014. Results. A total of 245 patients with Down syndrome were evaluated during the study period. Eighteen eyes of 15 patients (6.1% with RRD were identified. Three out of 15 patients (20% presented with bilateral retinal detachment. All eyes presented with macula off retinal detachment. The retina was successfully reattached in 16/18 (88.8% eyes after a mean follow-up of 48 months. The final postoperative visual acuity ranged from light perception to 20/125 (median: hand motion (11/18 eyes. Conclusions. The anatomic success rate of retinal reattachment surgery in patients with Down syndrome is comparable to the general population. Patients with Down syndrome should undergo regular ophthalmic examinations for early diagnosis. Despite late diagnosis and the presence of proliferative vitreoretinopathy (PVR in some patients, favorable anatomical outcomes can be achieved.

  1. Quantitative microbiological analysis of bacterial community shifts in a high-rate anaerobic bioreactor treating sulfite evaporator condensate.

    Science.gov (United States)

    Ney, U; Macario, A J; Conway de Macario, E; Aivasidis, A; Schoberth, S M; Sahm, H

    1990-08-01

    The bacterial population of a high-rate, anaerobic, fixed-bed loop reactor treating sulfite evaporator condensate from the pulp industry was studied over a 14-month period. This period was divided into seven cycles that included a startup at the beginning of each cycle. Some 82% of the total biomass was immobilized on and between the porous glass rings filling the reactor. The range of the total number of microorganisms in these biofilms was 2 x 10 to 7 x 10 cells per ml. Enumeration and characterization by microbiological methods and by phase-contrast, epifluorescence, and electron microscopy showed that the samples consisted mainly of the following methanogens: a Methanobacterium sp., a Methanosarcina sp., a Methanobrevibacter sp., and a Methanothrix sp., as well as furfural-degrading sulfate-reducing bacteria resembling Desulfovibrio furfuralis. Viable counts of hydrogenotrophic methanogens were relatively stable (mostly within the range of 3.2 x 10 to 7.5 x 10 cells per ml), but Methanobrevibacter cells increased from fixed bed into a second reactor vessel. Acetotrophic methanogens reached their highest numbers of 1.3 x 10 to 2.6 x 10 cells per ml in the last fermentation cycles. They showed a morphological shift from sarcinalike packets in early samples to single coccoid forms in later phases of the fermentation. Furfural-degrading sulfate reducers reached counts of 1 x 10 to 5.8 x 10 cells per ml. The distribution of the chief metabolic groups between free fluid and biofilms was analyzed in the fifth fermentation cycle: 4.5 times more furfural degraders were found in the free fluid than in the biofilms. In contrast, 5.8 times more acetotrophic and 16.6 times more hydrogenotrophic methanogens were found in the biofilms than in the free liquid. The data concerning time shifts of morphotypes among the trophic groups of methanogens corroborated the trends observed by using immunological assays on the same samples.

  2. Marfan Syndrome Presenting with Bilateral Retinal Detachment

    Directory of Open Access Journals (Sweden)

    Subrata Chakrabarti

    2014-02-01

    Full Text Available Marfan syndrome is an autosomal dominant systemic disorder of the connective tissue. Marfan syndrome affects most organs and tissues, especially the skeleton, lungs, eyes, heart, and the large blood vessels. Eye involvement may be in the form of retinal detachment which is a potentially dangerous manifestation for its sight threatening nature .We report a case where a 17 year old male developed sudden blindness due to spontaneous bilateral retinal detachment. Examination revealed features of Marfan syndrome and was stamped as a case of Marfan syndrome by Ghent criteria . The point to stress upon is that a young male developing spontaneous retinal detachment, a diagnosis of underlying Marfan syndrome should be kept in mind if appropriate clinical stigmata are present. [Natl J Med Res 2014; 4(1.000: 104-105

  3. [Binocular vision after treatment of retinal detachment].

    Science.gov (United States)

    Maksymowicz, Małgorzata; Raczyńska, Krystyna; Maksymowicz, Jarosław

    2003-01-01

    The study covered 79 patients after treatment of retinal detachment. Double vision, strabismus and disturbances of eyeballs motility were found. Up to 12 months after intervention, the deterioration of binocular vision was observed in 48.28 to 89.66% of patients, depending on the method used. The majority of disturbances were observed during the first 3 months with tendency to gradual subsidence during consecutive 9 months. A patient, after treatment of retinal detachment, can be qualified to return to work where stereopsis is needed under condition that ophthalmologic examination is done every three months during the first year after operation and than once a year.

  4. The DEBCat detached eclipsing binary catalogue

    CERN Document Server

    Southworth, John

    2014-01-01

    Detached eclipsing binary star systems are our primary source of measured physical properties of normal stars. I introduce DEBCat: a catalogue of detached eclipsing binaries with mass and radius measurements to the 2% precision necessary to put useful constraints on theoretical models of stellar evolution. The catalogue was begun in 2006, as an update of the compilation by Andersen (1991). It now contains over 170 systems, and new results are added on appearance in the refereed literature. DEBCat is available at: http://www.astro.keele.ac.uk/jkt/debcat/

  5. Characteristics of divertor detachment for ITER conditions

    Energy Technology Data Exchange (ETDEWEB)

    Kukushkin, A.S., E-mail: andre.kukushkin@iter.org [ITER Organization, St Paul Lez Durance (France); Pacher, H.D. [INRS-EMT, Varennes, Québec (Canada); Pitts, R.A. [ITER Organization, St Paul Lez Durance (France)

    2015-08-15

    The relative role of particle balance vs. momentum balance in the phenomenon of divertor plasma detachment in tokamaks is re-assessed. Ion removal from the plasma flow by volumetric recombination and/or cross-field transport is identified as the key element in the formation of the rollover of the ion saturation current on the targets, whereas “momentum removal” (friction) is responsible for maintaining high plasma pressure upstream. The deterioration of neutral particle confinement in the divertor as particle throughput increases is the primary cause of the solution collapse typically seen when deep detachment is modelled for present day experiments.

  6. High Frequency Color Doppler Image of Choroidal Detachment

    Institute of Scientific and Technical Information of China (English)

    Jinghong Wu; Lijuan Zou; Zhongyao Wu; Lixun Cheng

    2000-01-01

    Purpose: To study the Color Doppler Image (CDI) characteristics of choroidal detachment and the applied value of CDI.Methods: Seventy-two cases (74 eyes) of choroidal detachment were studied retrospectively.Results: The typical ultragraph of chroridal detachment displayed one or several smooth hemispherical or lobuler circular thick bands, with convex side toward vitreous cavity. Most of the choroidal detachments were located before the equator, a few of them were beyond the equator. CDI displayed blood flow singnal in the band. Pulse Doppler showed the frequency spectrum features of retinal detachment band were similar to those of central retinal vessels, whereas the frequency spectum features of choroidal detachment bend resembled those of ciliary artery in some cases of retinal detachment (RD) accompanied by choroidal detachment.Conclusion: CDI could make a correct and precise diagnosis of choroidal detachment.Eye Science 2000; 16. 61 ~ 64.

  7. A systematic review and meta-analysis of the association between systemic fluoroquinolones and retinal detachment.

    Science.gov (United States)

    Alves, Carlos; Penedones, Ana; Mendes, Diogo; Batel Marques, Francisco

    2016-08-01

    Several pharmacoepidemiologic studies have been carried out evaluating the risk of retinal detachment associated with systemic fluoroquinolones. This meta-analysis aims to investigate such association, in the light of the best scientific evidence available. A literature search was conducted to identify relevant studies evaluating the risk for retinal detachment associated with systemic fluoroquinolones. A meta-analysis was performed to pool rate ratios (RRs). Meta-regressions were conducted aiming to evaluate the influence of time interval between fluoroquinolones use and retinal detachment diagnosis or treatment risk estimates. Ten observational studies from seven publications were included. Overall, fluoroquinolones were not associated with an increased risk for retinal detachment [RR 1.47 (95% CI 0.95-2.27): p = 0.09; I(2)  = 92.8%]. When the analysis was stratified according to different study designs, the result was statistically significant for retrospective cohort studies [RR 1.87 (95% CI 1.36-2.58); p fluoroquinolones, based on data from case-control studies [RR 1.07 (95% CI 1.01-1.12); p = 0.01; I(2)  = 0.0%]. According to meta-regressions, the risk for retinal detachment did not vary due to different time intervals between fluoroquinolones prescription and retinal detachment occurrence. No statistically significant results were identified among studies evaluating only rhegmatogenous retinal detachments, as well as among studies that evaluated patients not requiring a prior ophthalmologist visit to be included. In light of the current available evidence, systemic fluoroquinolones do not seem to be associated with retinal detachment. © 2016 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.

  8. Low-energy electron attachment and detachment in vibrationally excited oxygen

    Science.gov (United States)

    Aleksandrov, N. L.; Anokhin, E. M.

    2009-11-01

    Three-body electron attachment to O2 molecules and electron detachment from O_{2}^{-} ions have been theoretically studied in vibrationally excited oxygen and O2-containing mixtures. Assuming that electron attachment and detachment proceed via the formation of vibrationally excited temporary O_{2}^{-} ions, the rates of these processes were determined on the basis of the statistical approach for the vibrational transfer and relaxation in collisions between O_{2}^{-} ions and O2 molecules. The calculated attachment and detachment rate constants turned out to agree well with available measurements in unexcited oxygen. This method was extended to calculate attachment and detachment rates in vibrationally excited oxygen. It was shown that the effect of vibrational excitation on electron detachment is profound, whereas attachment of low-energy electrons to vibrationally excited O2 is inefficient. The calculated vibrational distribution of stable O_{2}^{-} ions turned out to be non-equilibrium in an excited gas and the effective vibrational temperature of the ions was much lower than the vibrational temperature of molecules. An analytical method was suggested to determine this distribution and the effective vibrational temperature. The calculated rate constants were used to simulate the formation and decay of an electron-beam-generated plasma in N2 : O2 mixtures at elevated vibrational temperatures. The calculations showed that vibrational excitation of molecules leads to orders of magnitude increase in the plasma density and in the plasma lifetime, in agreement with available observations.

  9. Evaluation of the selective detachment process in flotation froth

    Energy Technology Data Exchange (ETDEWEB)

    Honaker, R.Q.; Ozsever, A.V. [University of Kentucky, Lexington, KY (United States). Dept. for Mining Engineering

    2003-10-01

    The improved selectivity between particles of varying degrees of hydrophobicity in flotation froths has been well documented in literature, especially in the deep froths utilized in flotation columns. The phenomenon is believed to be due to the selective detachment process whereby the least hydrophobic particles are released from the bubble surface upon bubble coalescence. To quantify the selective detachment process, column flotation experiments were performed under various operating conditions that provided varying amounts of reflux between the froth and collection zones. The flotation column incorporated the ability to provide instantaneous stoppage of the process streams and separation between the collection and froth zones after ensuring steady-state operation of the column. The samples collected from the two zones and process streams were evaluated to quantify the flotation rate distribution of the particles comprising each sample. The flotation rate was used as an indicator of the degree of hydrophobicity and thus a relative measure of the binding force between the particle and bubble in the froth zone. The flotation rate data was used as input into well known flotation models to obtain the froth zone recovery rate and the quantity of material that refluxes between the collection and froth zones.

  10. Device-associated infection rates and bacterial resistance in six academic teaching hospitals of Iran: Findings from the International Nocosomial Infection Control Consortium (INICC).

    Science.gov (United States)

    Jahani-Sherafat, Somayeh; Razaghi, Maryam; Rosenthal, Victor D; Tajeddin, Elahe; Seyedjavadi, Simasadat; Rashidan, Marjan; Alebouyeh, Masoud; Rostampour, Maryam; Haghi, Arezo; Sayarbayat, Masoumeh; Farazmandian, Somayeh; Yarmohammadi, Tahere; Arshadi, Fardokht K; Mansouri, Nahid; Sarbazi, Mohammad R; Vilar, Mariano; Zali, Mohammad R

    2015-01-01

    Device-associated health care-acquired infections (DA-HAIs) pose a threat to patient safety, particularly in the intensive care unit (ICU). However, few data regarding DA-HAI rates and their associated bacterial resistance in ICUs from Iran are available. A DA-HAI surveillance study was conducted in six adult and pediatric ICUs in academic teaching hospitals in Tehran using CDC/NHSN definitions. We collected prospective data regarding device use, DA-HAI rates, and lengths of stay from 2584 patients, 16,796 bed-days from one adult ICU, and bacterial profiles and bacterial resistance from six ICUs. Among the DA-HAIs, there were 5.84 central line-associated bloodstream infections (CLABs) per 1000 central line-days, 7.88 ventilator-associated pneumonias (VAPs) per 1000 mechanical ventilator-days and 8.99 catheter-associated urinary tract infections (CAUTIs) per 1000 urinary catheter-days. The device utilization ratios were 0.44 for central lines, 0.42 for mechanical ventilators and 1.0 for urinary catheters. The device utilization ratios of mechanical ventilators and urinary catheters were higher than those reported in the ICUs of the INICC and the CDC's NHSN reports, but central line use was lower. The DA-HAI rates in this study were higher than the CDC's NHSN report. However, compared with the INICC report, the VAP rate in our study was lower, while the CLAB rate was similar and the CAUTI rate was higher. Nearly 83% of the samples showed a mixed-type infection. The most frequent pathogens were Acinetobacter baumannii, Staphylococcus aureus and Pseudomonas aeruginosa, followed by Klebsiella pneumoniae and Enterococcus spp. In the S. aureus isolates, 100% were resistant to oxacillin. Overall resistances of A. baumannii and K. pneumonia to imipenem were 70.5% and 76.7%, respectively. A multiple drug resistance phenotype was detected in 68.15% of the isolates. The DA-HAI rates in Iran were shown to be higher than the CDC-NHSN rates and similar to the INICC rates

  11. [Retinal detachment with retinoschisis--case report].

    Science.gov (United States)

    Cristescu, R; Muşat, O; Toma, Oana; Coma, Corina; Gabej, Ioana; Burcea, M

    2013-01-01

    We present the case of a 43 year old patient diagnosed with rhegmatogenous retinal detachment and retinoschizis, a rare case of disease association. Surgery is recommended and we practice 23 gauge vitrectomy, laser retinopexy, criopexy in the periphery and internal heavy oil tamponade. Postoperatory evolution was favorable.

  12. Modeling Detached Plasmas in DIII-D

    Science.gov (United States)

    Porter, Gary D.; Rognlien, T. D.; Rensink, M. E.; DIII-D Team

    1996-11-01

    The ITER divertor design relies on operation of the machine with a detached divertor plasma as a means of reducing the divertor heat load to manageable levels. This operating mode has been seen on all of the world's diverted tokamaks, and is characterized by very low plate temperatures and ion currents. Experimental results on DIII-D have shown the plate electron temperature is between 1 and 2 eV. We describe the results of modeling these detached plasmas with the UEDGE code in this paper. Plasma detachment can be achieved in a variety of ways in the code as well as in experiment. Simulations indicate the detachment process occurs in two steps: a thermal collapse in which the plate temperature drops to 1 to 2 eV, followed by a decrease in the plate ion current. When the low temperature region extends off the plate, parallel momentum of the plasma is reduced by ion/neutral interactions. The plate ion current decreases when the parallel momentum is reduced sufficiently to permit volume recombination processes to compete with ion flow to the plate.

  13. Inactivation rates of Cronobacter spp. and selected other bacterial strains in powdered infant formulae stored at different temperatures

    NARCIS (Netherlands)

    Kandhai, M.C.; Reij, M.W.; Schothorst, van M.; Gorris, L.G.M.; Zwietering, M.H.

    2010-01-01

    The aim of this study was to determine the survival of two strains of Cronobacter (Enterobacter sakazakii) and six other bacterial strains inoculated into dry powdered infant formula (PIF) stored for 22 weeks at several temperatures between 7 and 42°C. The experimental setup involved a relatively hi

  14. The Factors Affecting Success of Pneumatic Retinopexy in Treatment of Retinal Detachment

    Directory of Open Access Journals (Sweden)

    Kemal Yüksel

    2016-06-01

    Full Text Available Objective: To present the factors that influence the suc­cess rate of pneumatic retinopexy on rhegmatogenous retinal detachment treatment. Methods: Ninety eyes of 29 female and 61 male patient were included to the study. The patient’s age, sex, du­ration of symptoms, the best corrected visual acuities (BCVA on the time of application, lens status, preopera­tive retinal tear number and location, retinal detachment size, macular involvement, tamponade type, coexisting complications, and anatomic success rates were record­ed at preoperative, postoperative 3rd months and final examination. Results: Only one treatment session success number was 55 over 90 (61.1% patients. Median preoperative, postoperative 3rd month and final examination Log­MAR BCVA values were 1.3, 0.5 and 0.5 respectively.(p=0.0001 Preoperative and postoperative other param­eters was not revealed significant differences with one treatment session. However there was a significant posi­tive correlation between the detachment size and ana­tomical success. (p=0.03, r=0.324 Conclusion: Pneumatic retinopexy is an alternative treat­ment option for the anatomical and functional success in retinal detachment at suitable patients. In our study the success rate was higher in patients with less than 3 clock dial retinal detachment patients.

  15. Bacterial growth rates, production and estimates of detrital carbon utilization in deep-sea sediments of the Solomon and Coral Seas

    Science.gov (United States)

    Alongi, Daniel M.

    1990-05-01

    Surface (0-5 mm) sediments at 10 bathyal and abyssal (695-4350 m) stations were sampled to determine variations in bacterial densities, productivity (tritiated thymidine incorporation at simulated in situ conditions) and specific growth rates (μ) in relation to environmental conditions and phytodetritus in the Solomon and Coral Seas. bacterial direct counts ranged from 2.5 to 60.6 × 10 8 cells g -1 sediment dry wt, but did not decline significantly with ocean depth or correlate with any other variables measured. Bacterial productivity was generally low, ranging from 34 to 7010 μg C m -2 d -1, but specific growth rates varied widely, ranging from 0.001 to 0.12 d -1. Incubation of samples at in situ temperature and atmospheric pressure at two stations resulted in either no detectable growth or significantly decreased thymidine incorporation, indicating a barophilic response. Quantities of macroalgal detritus were found at stations closest to reefs, whereas vascular plant and wood debris were found at most of the other stations, indicating that some detrital material transported from adjacent reefs and by massive riverine export from Papua New Guinea reaches the deep-sea floor in both seas. Significant quantities of sedimentary chlorophyll α and phaeo-pigments also were detected, indicating some deposition of phytoplankton-derived detritus. The fluxes of phytoplankton-derived, detrital carbon to the benthos were estimated (using available plankton data and the flux equation of Suess) as on the order of 2-23 mg C m -2 d -1. Assuming a median carbon assimilation efficiency of 50%, utilization of this material by sedimentary bacteria was estimated to vary widely (2-97%), averaging 40% oof total detrital carbon flux. Rates of bacterial productivity declined significantly with water depth and correlated with no other factors, but specific growth rates correlated positively with the standing amounts of macroalgal debris and were comparatively rapid (= turnover times of

  16. How Cosmic Web Detachment Drives Galaxy Quenching

    CERN Document Server

    Aragon-Calvo, Miguel A; Silk, Joseph

    2016-01-01

    We present the Cosmic Web Detachment (CWD) model, a conceptual framework to interpret galaxy evolution in a cosmological context, providing a direct link between the star formation history of galaxies and the cosmic web. The CWD model unifies several mechanism known to disrupt or stop star formation into one single physical process and provides a natural explanation for a wide range of galaxy properties. Galaxies begin accreting star-forming gas at early times via a network of primordial highly coherent filaments. The efficient star formation phase ends when non-linear interactions with other galaxies or elements of the cosmic web detach the galaxy from its network of primordial filaments, thus ending the efficient accretion of cold gas. The stripping of the filamentary web around galaxies is the physical process responsible of star formation quenching in gas stripping, harassment, strangulation and starvation. Being a purely gravitational/mechanical process CWD acts at a more fundamental level than internal ...

  17. Mechanism of bubble detachment from vibrating walls

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Dongjun; Park, Jun Kwon, E-mail: junkeun@postech.ac.kr; Kang, Kwan Hyoung [Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Pohang 790-784 (Korea, Republic of); Kang, In Seok [Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Pohang 790-784 (Korea, Republic of)

    2013-11-15

    We discovered a previously unobserved mechanism by which air bubbles detach from vibrating walls in glasses containing water. Chaotic oscillation and subsequent water jets appeared when a wall vibrated at greater than a critical level. Wave forms were developed at water-air interface of the bubble by the wall vibration, and water jets were formed when sufficiently grown wave-curvatures were collapsing. Droplets were pinched off from the tip of jets and fell to the surface of the glass. When the solid-air interface at the bubble-wall attachment point was completely covered with water, the bubble detached from the wall. The water jets were mainly generated by subharmonic waves and were generated most vigorously when the wall vibrated at the volume resonant frequency of the bubble. Bubbles of specific size can be removed by adjusting the frequency of the wall's vibration.

  18. Effect of Biofilm Detachment on Nitrification at Low C/N Ratio%低C/N值条件下生物膜脱落对硝化作用的影响

    Institute of Scientific and Technical Information of China (English)

    殷峻; 徐恒娟

    2013-01-01

    Biofilm was grown at constant flow rate and low C/N ratio in a tube biofilm reactor. Detachment experiment was performed by temporarily increasing the influent flow rate into the reactor to e-valuate the effect of mixed-population biofilm detachment on the nitrification process. Changes in the composition of biofilm were examined by denaturing gradient gel electrophoresis ( DGGE). The biofilm thickness was determined. The results showed that ammonium oxidation rate reached above 99% after three weeks. Then, nitrate was detected. However, during the experiment, nitrite was observed in the effluent all the time. Biofilm detachment could cause the increase of nitrite concentration in the effluent. Detachment events happened mainly under constant hydrodynamic conditions, even though flow rate in the tube was increased to 4 000 mL/min, which did not cause a significant biofilm detachment. DGGE fingerprints showed that microbial population in attached biofilm was similar to that in detached biofilm, indicating that there was no significant shift in the composition of bacterial population throughout the experiment. The intensity of some bands in detached biofilm was higher than that in attached biofilm. Therefore, biofilm detachment could induce change in bacterial abundance in biofilms and then influence the nitrification process.%利用管式生物膜反应器,在恒定水力和低C/N值条件下培养生物膜,当系统稳定后,通过暂时增大反应器内水流流速的方式进行膜脱落试验,以评价在混合菌生物膜内生物膜脱落对硝化过程的影响.采用变性梯度凝胶电泳(DGGE)技术检测生物膜菌群组成的变化,同时测定生物膜的厚度.结果表明,在试验开始3周后氨氧化率就达到了99%,且在出水中检测到了硝酸盐.不过,在整个试验过程中出水一直能检测到一定浓度的亚硝酸盐.试验发现,生物膜脱落会引起出水亚硝酸盐浓度的增加.生物膜脱落主要发生在恒定

  19. [Treatment of retinal detachment with macular hole].

    Science.gov (United States)

    Pikulski, Z; Nawrocki, J; Dziegielewski, K

    1993-01-01

    The methods and results of surgery in 6 cases of retinal detachment with macular hole are presented. In all 6 cases pars plana vitrectomy was performed, in 4 with subsequent SF6 and in 2 with silicone oil tamponade. Retinal attachment was achieved in 4 eyes. Visual acuity 1/50-2/50 was found after surgery in 5 cases. The follow-up ranged from 6 to 9 months.

  20. Tonic shock induces detachment of Giardia lamblia.

    Directory of Open Access Journals (Sweden)

    Wendy R Hansen

    Full Text Available BACKGROUND: The parasite Giardia lamblia must remain attached to the host small intestine in order to proliferate and subsequently cause disease. However, little is known about the factors that may cause detachment in vivo, such as changes in the aqueous environment. Osmolality within the proximal small intestine can vary by nearly an order of magnitude between host fed and fasted states, while pH can vary by several orders of magnitude. Giardia cells are known to regulate their volume when exposed to changes in osmolality, but the short-timescale effects of osmolality and pH on parasite attachment are not known. METHODOLOGY AND PRINCIPAL FINDINGS: We used a closed flow chamber assay to test the effects of rapid changes in media osmolality, tonicity, and pH on Giardia attachment to both glass and C2(Bbe-1 intestinal cell monolayer surfaces. We found that Giardia detach from both surfaces in a tonicity-dependent manner, where tonicity is the effective osmolality experienced by the cell. Detachment occurs with a characteristic time constant of 25 seconds (SD = 10 sec, n = 17 in both hypo- and hypertonic media but is otherwise insensitive to physiologically relevant changes in media composition and pH. Interestingly, cells that remain attached are able to adapt to moderate changes in tonicity. By exposing cells to a timed pattern of tonicity variations and adjustment periods, we found that it is possible to maximize the tonicity change experienced by the cells, overcoming the adaptive response and resulting in extensive detachment. CONCLUSIONS AND SIGNIFICANCE: These results, conducted with human-infecting Giardia on human intestinal epithelial monolayers, highlight the ability of Giardia to adapt to the changing intestinal environment and suggest new possibilities for treatment of giardiasis by manipulation of tonicity in the intestinal lumen.

  1. Extinguishing ELMs in detached radiative divertor plasmas

    Science.gov (United States)

    Pigarov, Alexander; Krasheninnikov, Sergei; Rognlien, Thomas

    2016-10-01

    In order to avoid deleterious effects of ELMs on PFCs in next-step fusion devices it has been suggested to operate with small-sized ELMs naturally extinguishing in the divertor. Our modeling effort is focusing at extinguishing type-I ELMs: conditions for expelled plasma dissipation; efficiency of ELM power handling by detached radiative divertors; and the ELM impact on detachment state. Here time-dependent modeling of a sequence of many ELMs was performed with 2-D edge plasma transport code UEDGE-MB-W which incorporates the Macro-Blob (MB) approach to simulate non-diffusive filamentary transport and various ``Wall'' (W) models for time-dependent hydrogen wall inventory and recycling. Three cases were modeled, in which extinguishing ELMs are achieved due to: (i) intrinsic impurities via graphite sputtering, (ii) extrinsic impurity gas puff (Ne), and (iii) =(i) +(ii). For each case, we performed a series of UEDGE-MB-W runs scanning the deuterium and impurity inventories, pedestal losses and ELM frequency. Temporal variations of the degree of detachment, ionization front shape, recombination sink strength, radiated fraction, peak power loads, OSP, impurity charge states, and in/out asymmetries were analyzed. We discuss the onset of extinguishing ELMs, conditions for not burning through and enhanced plasma recombination as functions of scanned parameters. Efficiencies of intrinsic and extrinsic impurities in ELM extinguishing are compared.

  2. Fermentative hydrogen production and bacterial community structure in high-rate anaerobic bioreactors containing silicone-immobilized and self-flocculated sludge.

    Science.gov (United States)

    Wu, Shu-Yii; Hung, Chun-Hsiung; Lin, Chi-Neng; Chen, Hsin-Wei; Lee, An-Sheng; Chang, Jo-Shu

    2006-04-01

    A novel continuously stirred anaerobic bioreactor (CSABR) seeded with silicone-immobilized sludge was developed for high-rate fermentative H2 production using sucrose as the limiting substrate. The CSABR system was operated at a hydraulic retention time (HRT) of 0.5-6 h and an influent sucrose concentration of 10-40 g COD/L. With a high feeding sucrose concentration (i.e., 30-40 g COD/L) and a short HRT (0.5 h), the CSABR reactor produced H2 more efficiently with the highest volumetric rate (VH2) of 15 L/h/L (i.e., 14.7 mol/d/L) and an optimal yield of ca. 3.5 mol H2/mol sucrose. The maximum VH2 value obtained from this work is much higher than any other VH2 values ever documented. Formation of self-flocculated granular sludge occurred during operation at a short HRT. The granule formation is thought to play a pivotal role in the dramatic enhancement of H2 production rate, because it led to more efficient biomass retention. A high biomass concentration of up to 35.4 g VSS/L was achieved even though the reactor was operated at an extremely low HRT (i.e., 0.5 h). In addition to gaining high biomass concentrations, formation of granular sludge also triggered a transition in bacterial community structure, resulting in a nearly twofold increase in the specific H2 production rate. According to denatured-gradient-gel-electrophoresis analysis, operations at a progressively decreasing HRT resulted in a decrease in bacterial population diversity. The culture with the best H2 production performance (at HRT = 0.5 h and sucrose concentration = 30 g COD/L) was eventually dominated by a presumably excellent H2-producing bacterial species identified as Clostridium pasteurianum.

  3. Can a New Antiseptic Agent Reduce the Bacterial Colonization Rate of Central Venous Lines in Post- Cardiac Surgery Patients?

    Directory of Open Access Journals (Sweden)

    Fardin Yousefshahi

    2015-10-01

    Full Text Available Background: Central venous (CV catheters play an essential role in the management of critically ill patients in the Intensive Care Unit (ICU. CV lines are, however, allied to catheter-associated blood stream infections. Bacterial colonization of CV lines is deemed the main cause of catheter-associated infection. The purpose of our study was to compare bacterial colony counts in the catheter site before CV line insertion in two groups of post-cardiac surgery patients: a group receiving Sanosil (an antiseptic agent composed of H2 O2 and silver and a control group.Methods: This interventional prospective double-blinded clinical trial recruited the patients in three post-cardiac surgeryICUs of a heart center. The participants were divided into interventional (113 patients and control (136 patients groups. Sanosil was added to the routine preparation procedure (Chlorhexidine bath one day before and scrub with Povidone-Iodine just before the CV line insertion. After the removal of the CV lines, the catheters tips were sent for culture and evaluation of colony counts.Results: Catheter colonization occurred in 55 (22.1% patients: 26 (23% patients in the Sanosil group and 29 (21.3% in the control group; there was no significant statistical difference between the two groups (p value = 0.75, RR = 1.05, 95%CI:0.76-1.45. The most common organism having colonized in the cultures of the catheter tips was staphylococcus epidermis:20 cases in the control group and 16 cases in the intervention group.Conclusion: Catheter colonization frequently occurs in post-cardiac surgery patients. However, our results did not indicate the effectiveness of adding Sanosil to the routine preparation procedure with respect to reducing catheter bacterial colonization.

  4. Selective reactivity of monochloramine with extracellular matrix components affects the disinfection of biofilm and detached clusters.

    Science.gov (United States)

    Xue, Zheng; Lee, Woo Hyoung; Coburn, Kimberly M; Seo, Youngwoo

    2014-04-01

    The efficiency of monochloramine disinfection was dependent on the quantity and composition of extracellular polymeric substances (EPS) in biofilms, as monochloramine has a selective reactivity with proteins over polysaccharides. Biofilms with protein-based (Pseudomonas putida) and polysaccharide based EPS (Pseudomonas aeruginosa), as well as biofilms with varied amount of polysaccharide EPS (wild-type and mutant P. aeruginosa), were compared. The different reactivity of EPS components with monochloramine influenced disinfectant penetration, biofilm inactivation, as well as the viability of detached clusters. Monochloramine transport profiling measured by a chloramine-sensitive microelectrode revealed a broader diffusion boundary layer between bulk and biofilm surface in the P. putida biofilm compared to those of P. aeruginosa biofilms. The reaction with proteins in P. putida EPS multiplied both the time and the monochloramine mass required to achieve a full biofilm penetration. Cell viability in biofilms was also spatially influenced by monochloramine diffusion and reaction within biofilms, showing a lower survival in the surface section and a higher persistence in the middle section of the P. putida biofilm compared to the P. aeruginosa biofilms. While polysaccharide EPS promoted biofilm cell viability by obstructing monochloramine reactive sites on bacterial cells, protein EPS hindered monochloramine penetration by reacting with monochloramine and reduced its concentration within biofilms. Furthermore, the persistence of bacterial cells detached from biofilm (over 70% for P. putida and ∼40% for polysaccharide producing P. aeruginosa) suggested that currently recommended monochloramine residual levels may underestimate the risk of water quality deterioration caused by biofilm detachment.

  5. Coastal Response, a system of detached breakwaters

    Science.gov (United States)

    García Ortiz, Isabelo; Negro Valdecantos, Vicente; Santos López, Jose; Esteban, María Dolores

    2017-04-01

    The coastline's sedimentary response in the form of a tombolo or semi-tombolo (salient) as a result of the construction of detached breakwaters is an aspect that should be known in the design phase so that these marine structures may be properly designed. In achieving an ecological, social and economic value, such areas must also be properly managed. All design methods in existence since Dean (1978) are mainly based on hypotheses formulated from geometric studies on existing formations. No relationship at all is established with climate and littoral dynamics typical of the location (only Suh and Darlymple (1987) and the Japanese Ministry of Construction (1986) present relationships depending on wave variables). Neither has the influence on systems with more than two breakwaters been studied. These methods are not fully adapted to the cases existing on the Spanish Mediterranean littoral. The lines of investigation as proposed by L. Bricio and V. Negro (2010) were continued with for this study. These researchers developed a method for dimensioning isolated, detached breakwaters and their semi-tombolo or tombolo associated formations using all the characteristics of the site (energy, geometric and structural), specific climate and geomorphology and littoral dynamics' characteristics. This methodology is currently acknowledged and accepted in works undertaken on the Spanish Mediterranean littoral. A linear regression was obtained in the investigation undertaken on the 18 detached breakwater systems along the whole of the 1670 km of the Spanish Mediterranean littoral using the proposals made by L. Bricio and V. Negro. The adjustment of R2 ≥ 0.90 was used for the sandy, tombolo formations behind all the detached breakwater systems between several non-dimensional monomials displaying the most representative characteristics of the site. L/H12 + (2ṡB)/G =12,15ṡ(X/Xc)+7,3231 X: Distance of breakwaters from coastline Xc: Distance from coastline where the closure depth

  6. Seasonal capacity of attached and detached vineyard roots to support grape phylloxera (Homoptera: Phylloxeridae).

    Science.gov (United States)

    Granett, J; Omer, A D; Walker, M A

    2001-02-01

    Field experiments were conducted to evaluate population densities and survival, developmental rate, and fecundity of grape phylloxera, Daktulosphaira vitifoliae (Fitch), as influenced by root attachment or detachment from mature, field-grown, Vitis vinifera L. grapevines through the growing season. Experiments were performed using artificial infestations of California biotype A grape phylloxera. Thirty-day bioassays on attached- and detached-roots were repeated monthly from May to September in 1997 (cultivar 'Carignane') and April to September in 1998 (cultivar 'Thompson Seedless'). The bioassays showed that attached roots had lower population densities than detached roots in all months of both years. Densities varied by month, tending to be higher in spring than in summer. Of the population parameters studied, survival was most influenced by attachment condition, being higher on detached than on attached roots by up to 25-fold. These results imply the importance of vine-related mortality factors to grape phylloxera population density. Influence of root attachment condition on developmental rate and fecundity was not uniform across bioassay months for either year; however, in the four out of 21 assays where there was a significant difference it favored detached roots by twofold. Fruit harvest resulted in higher survival in the July assay but not for assays in August and September; however, neither developmental rate nor fecundity was affected by harvest in any ofthe assays. We conclude that mortality rather than nutritional factors are most limiting for field populations on susceptible vines. This work suggests that detachment of roots as occurs with root girdling by root pathogens may increase grape phylloxera populations on infested, susceptible vines. These results imply that excised root bioassays over-estimate grape phylloxera virulence and underestimate rootstock resistance.

  7. Primary 23-gauge sutureless vitrectomy for rhegmatogenous retinal detachment

    Directory of Open Access Journals (Sweden)

    Mario R Romano

    2012-01-01

    Full Text Available Aims : To report a prospective non-comparative consecutive interventional study on the safety and efficacy of 23-Gauge transconjunctival sutureless pars plana vitrectomy for primary rhegmatogenous retinal detachment (RRD. Materials and Methods: Fifty eyes of 50 consecutive patients were recruited between June 2007 and January 2008. All surgeries were performed using the one-step 23-Gauge system with angled incisions. The surgical protocol consisted of a minimum of eight clinical visits: baseline, 1 day, 1 week, 1-, 3- and 6- months after the initial surgery. The endpoints were anatomical, functional results and complications arising from the surgery. Results : Anatomical success was achieved in 82% of cases (41 out of 50 with single surgery and rose to 98% (49 out of 50 with additional surgery. Mean visual acuity improved from logMAR 0.48(SD 0.36 to 0.26(SD 0.31, P < 0.001. Two cases with ocular hypotony, defined as an intraocular pressure ≤ 6mmHg, that were associated with a choroidal detachment were seen. Conclusions : Acceptable anatomical and functional success rates can be achieved with primary 23-Gauge transconjunctival sutureless vitrectomy for RRD. We found that the approach technique is different from conventional vitrectomy and the complications arising from post surgical hypotony and leakage from sclerotomies are potentially higher compared to 20-Gauge vitrectomy.

  8. Forces involved in bacterial adhesion to hydrophilic and hydrophobic surfaces

    NARCIS (Netherlands)

    Boks, N.P.; Norde, W.; Meil, H.C.; Busscher, H.J.

    2008-01-01

    Using a parallel-plate flow chamber, the hydrodynamic shear forces to prevent bacterial adhesion (F-prev) and to detach adhering bacteria (F-det) were evaluated for hydrophilic glass, hydrophobic, dimethyldichlorosilane (DDS)-coated glass and six different bacterial strains, in order to test the

  9. Forces involved in bacterial adhesion to hydrophilic and hydrophobic surfaces

    NARCIS (Netherlands)

    Boks, Niels P.; Norde, Willem; van der Mei, Henny C.; Busscher, Henk J.

    2008-01-01

    Using a parallel-plate flow chamber, the hydrodynamic shear forces to prevent bacterial adhesion (F(prev)) and to detach adhering bacteria (F(det)) were evaluated for hydrophilic glass, hydrophobic, dimethyldichlorosilane (DDS)-coated glass and six different bacterial strains, in order to test the

  10. Forces involved in bacterial adhesion to hydrophilic and hydrophobic surfaces

    NARCIS (Netherlands)

    Boks, N.P.; Norde, W.; Meil, H.C.; Busscher, H.J.

    2008-01-01

    Using a parallel-plate flow chamber, the hydrodynamic shear forces to prevent bacterial adhesion (F-prev) and to detach adhering bacteria (F-det) were evaluated for hydrophilic glass, hydrophobic, dimethyldichlorosilane (DDS)-coated glass and six different bacterial strains, in order to test the fol

  11. Forces involved in bacterial adhesion to hydrophilic and hydrophobic surfaces

    NARCIS (Netherlands)

    Boks, Niels P.; Norde, Willem; van der Mei, Henny C.; Busscher, Henk J.

    2008-01-01

    Using a parallel-plate flow chamber, the hydrodynamic shear forces to prevent bacterial adhesion (F(prev)) and to detach adhering bacteria (F(det)) were evaluated for hydrophilic glass, hydrophobic, dimethyldichlorosilane (DDS)-coated glass and six different bacterial strains, in order to test the f

  12. Experimental Study of Single- and Double-Electron Detachment for Negative Carbon Ions Incident on Helium

    Institute of Scientific and Technical Information of China (English)

    HUANG Yong-Yi; WU Shi-Min; ZHANG Xue-Mei; LI Guang-Wu; LU Fu-Quan; YANG Fu-Jia

    2004-01-01

    Using the growth rate method, we obtain the single-electron detachment (SED) cross-sections for 5-30keV C-+He, and double-electron detachment (DED) cross-sections for 5-15keV C--+He. The SED cross-sections first increase with the increasing incident ion energy, and then decrease with further increase of the energy. The DED cross-sections increase with the increasing incident energy in the 5-15keV region.

  13. Temporal Variation in Splash Detachment at the Forest Floor of Japanese Cypress (Chamaecyparis obtusa) plantations

    Science.gov (United States)

    Wakiyama, Y.; Onda, Y.; Nanko, K.; Mizugaki, S.; Kitahara, H.; Ono, H.

    2008-12-01

    Temporal variation in each sub-process of soil erosion during rainfall is one of causes which add the difficulty in the prediction of their extents. However, monitoring such variation in each process had caused technical difficulties especially under natural rainfall conditions. This study presents temporal variations in splash detachment under natural rainfall using a piezoelectric saltation sensor, SENSIT (H11B; SENSIT. Co., USA) in Japanese cypress stand where splash erosion occurred triggered by raindrop with high energy. A laboratory test evaluating the applicability of SENSIT for field situations suggested that SENSIT can detect the impacts by splashed sediment. Field observations were conducted in 21yr- and 36yr-stand of Japanese cypress Chamaecyparis obtusa with mean stand"fs height of 9.2 m and 17.4 m, respectively. Splash sediment was collected at seven times using splash cup. Raindrop kinetic energy of each stand was measured using LD gauges. SENSIT was installed to monitor saltation counts, which were converted to temporal data of splash detachment (splash detachment rate; g m-2 10 min-1) with the relationship between splash detachment and cumulative saltation counts. Surface runoff was also monitored using runoff plots (0.5 m width × 2.0 m length) to discuss the effect of flow depth on splash detachment. Total splashed sediment during observations was 5100 g m-2 in 21yr-stand and 8192 g m-2 in 36yr-stand. Raindrop kinetic energy in 21yr-stand and 36yr-stand was 16.9 and 21.0 J m-2 mm-1, respectively. These results suggested that the growth of tree height in Japanese cypress stands increase splash detachment due to the increase of raindrop kinetic energy. During an extreme rainstorm event with 268 mm over 60 hours, splash detachment rate in 21yr-stand gradually decreased whereas that in 36yr-stand increased with time. In contrast, surface runoff appeared as low runoff rate in 21yr-stand and gradual increase with time in 36yr-stand. These results

  14. Post-operative hospitalization in retinal detachment correlation to recurrences

    Directory of Open Access Journals (Sweden)

    Enzo Maria Vingolo

    2013-12-01

    Full Text Available PURPOSE: To evaluate relationships between the incidence of re-detachment and postoperative days of hospitalization in patients with diagnosis of retinal detachment (RD who underwent surgery either through ab-externo approach (scleral buckling and encircling or ab-interno approach (vitrectomy and oil-gas tamponade. METHODS: This retrospective study included 268 patients (268 eyes with diagnosis of primary RD: 127 males (47% and 141 (53% females, mean age of 64.1 ± 17.3 years. 46 patients (17% underwent a surgical ab-externo approach (group A, while 222 patients (83% underwent an ab-interno surgical approach. Each RD was graded according to the "Retinal detachment grading system of Royal College of Ophthalmologists" and treated within 5 days after diagnosis. The redetachment (RT related to a surgical failure was considered within 30 days after first operation. RESULTS: In the group 1 without night hospitalization (day surgery global RT rate was 3.5% (4.17% for ab-interno technique and 0% for ab-externo techniques. In the group 2 with one day of hospitalization global RT rate was 1.33% (1.49% for ab-interno technique and 0% for ab-externo techniques. In the group 3 with 2 days of hospitalization global RT rate was 3.80% (4.62% for ab-interno techniques and 0% for ab-externo techniques. In the group 4 with 3 or more days of hospitalization global RT rate was 12.28% (14.29% for ab-interno techniques for 6.67% ab-externo techniques. The incidence of RT in group 1, day surgery, is comparable to the other groups and it is less compared to group 4 (3 or more days of hospitalization. CONCLUSIONS: The incidence of retinal redetachment (RRD is not increased in day surgery mode. These data support outpatient management of RD patients to reduce medical cost and problems related to the hospitalization. Further studies are needed for patients' security.

  15. Therapeutic effect of external-route microsurgery for rhegmatogenous retinal detachment

    Directory of Open Access Journals (Sweden)

    Jian-Xun Zhu

    2014-07-01

    Full Text Available AIM: To study the therapeutic effect of external-route microsurgery forrhegmatogenous retinal detachment.METHODS: In 55 patients(55 eyeswith rhegmatogenous retinal detachment, drainage of subretinal fluid, examination of locating the holes, sclera cryotherapy, scleral buckling, and vitreous cavity injection of filtrated air were performed under surgical microscope.RESULTS: The retinal reattachment occurred in 50 cases after the primary surgery. The final rate of reattachment was 91% during 6-12mo follow-up. The retinal reattachment occurred in 1 case(recurrent retinal detachmentafter the secondary surgery and in 4 cases(recurrent retinal detachmentafter vitrectomy. The eyesight was improved with different degrees in 55 cases.CONCLUSION: The external-route microsurgery for rhegmatogenous retinal detachment is simple, safe and effective.

  16. Bacteriophage PRD1 batch experiments to study attachment, detachment and inactivation processes

    NARCIS (Netherlands)

    Sadeghi, G.; Schijven, J.F.; Behrends, T.; Hassanizadeh, S.M.; van Genuchten, M.T.

    2013-01-01

    Knowledge of virus removal in subsurface environments is pivotal for assessing the risk of viral contamination of water resources and developing appropriate protection measures. Columns packed with sand are frequently used to quantify attachment, detachment and inactivation rates of viruses. Since

  17. Detachment force of particles from fluid droplets.

    Science.gov (United States)

    Ettelaie, Rammile; Lishchuk, Sergey V

    2015-06-07

    We calculate the deformation of a spherical droplet, resulting from the application of a pair of opposite forces to particles located diametrically opposite at the two ends of the droplet. The free-energy analysis is used to calculate the force-distance curves for the generated restoring forces, arising from the displacement of the particles relative to each other. While the logarithmic dependence of the "de Gennes-Hooke" constant on the particle to droplet size ratio, ν, is rather well known in the limit of very small ν, we find that for more realistic particle to droplet size ratios, i.e. ν = 0.001 to 0.01, the additional constant terms of O(1) constitute a significant correction to previously reported results. We derive the restoring force constant to be 2πγ[0.5 - ln (ν/2)](-1), in perfect agreement with the exact semi-numerical analysis of the same problem. The deviation from the linear force-displacement behaviour, occurring close to the point of detachment, is also investigated. A study of the energy dissipated shows it to be an increasingly dominant component of the work done during the detachment of the particles, as ν decreases. This indicates the existence of a significantly higher energy barrier to desorption of very small particles, compared to the one suggested by their adsorption energy alone. The influence of the line tension on the detachment force is also considered. It is shown that where line tension is important, the contact angle is no longer a constant but instead alters with the displacement of the particles from their equilibrium positions.

  18. Mediterranean detachment zones : thermicity vs heritage.

    Science.gov (United States)

    Labrousse, Loic; Huet, Benjamin; Le Pourhiet, Laetitia; Jolivet, Laurent; Burov, Evgenii

    2017-04-01

    Even if the seminal comprehensive descriptions of Metamorphic Core Complexes (MCCs) in the American Cordillera mentionned lower plates constituted of gneiss and intruded by granites (e. g. Snake Range, Whipple Mountains), the actual definition of MCCs : « Cordilleran metamorphic core complexes appear to be bodies from the middle crust that have been dragged out from beneath fracturing and extending upper crustal rocks, and exposed beneath shallow-dipping (normal slip) faults of large areal extent » {as in Lister & Davis, 1989, Journal of Structural Geology, v. 11, pp. 65-94} refers to rocks exhumed from the middle crust whatever their thermal history. The fundamental property of this middle crust resides in its ability fo flow lateraly toward the forming dome, to accommodate stretching of the upper plate and preserve a relatively flat moho. Even though thermal reequilibration can induce weakening of the lower crust, a similar strength profile can also be inherited from pre-extension evolution of the continental crust and promote development of the original structure of MCCs : their detachment. In order to unravel the rheological meaning of detachments, we propose here a review of extensional shear zones described as detachments in the Mediterranean realm, and establish a three end-members typology with « hot MCCs » as one end-member, and two cold MCC end-members with a weak middle crust due to stacking of high pressure metapelitic nappes or a strong upper crust responsible for the strength contrast exaggeration between the upper and lower crust. New fully coupled thermo-mechanical modeling experiments together with a review of comparable published results allow to test this three end-member typology and determine the critical strength constrast for the perennial development of a detachment zone. A 1000 ratio between the strength at the brittle-ductile transition and the strength at the base of the crust seems a boundary value between localized extensional modes

  19. From detached to attached buildup complexes

    DEFF Research Database (Denmark)

    Rafaelsen, B.; Elvebakk, G.; Andreassen, K.

    2008-01-01

    warm- and cool-water carbonate buildups from the Finnmark Platform is controlled by faults and the sea floor morphology at the time of their growth. A fluctuating sea level affected the growth of the carbonate buildups, but they also influenced their own environment by forming lagoons, atoll...... deposition and buildup growth bridged the detached platform with the attached platform. In the Bjarmeland Group (Lower Permian) 0.35-4.8 km wide, 1.5-27 km long and 60-420 m thick cool-water bryozoan-dominated straight, sinuous and continuous carbonate ridges or atoll-like ridges are located on top...

  20. Oral fluoroquinolones and the incidence of rhegmatogenous retinal detachment and symptomatic retinal breaks: a population-based study

    Science.gov (United States)

    Kapoor, Kapil G.; Hodge, David O.; St Sauver, Jennifer L.; Barkmeier, Andrew J.

    2016-01-01

    Objective To examine whether oral fluoroquinolone antibiotics are associated with an increase in subsequent rhegmatogenous retinal detachment and symptomatic retinal breaks in a large, population-based cohort. Design Population-based cohort study Participants and Controls Adult residents of Olmsted County, Minnesota who were prescribed oral fluoroquinolone medications from 1/01/03 – 6/30/11. Comparison cohorts consisted of patients prescribed oral macrolide and β-lactam antibiotics during the study period. Methods Procedure codes were used to identify retinal detachment repair and prophylaxis procedures occurring within 1 year of prescription dates. Travel clinic, pro re nata, and self-treatment prescriptions were excluded. Patients with tractional retinal detachment, previous retinal detachment repair, endophthalmitis, and necrotizing retinitis were excluded, as were those with intraocular surgery or severe head/eye trauma ≤ 90 days prior to the procedure. Main Outcome Measures Rates of retinal detachment repair and prophylaxis procedures within 7, 30, 90, and 365 days of the first prescription were calculated and compared between antibiotic prescription cohorts using Chi-square tests. Retinal detachment repair rates were also compared to the expected Olmsted County, Minnesota rates using the one-sample log rank test. Results Oral fluoroquinolones were prescribed for 38,046 patients (macrolide n=48,074, β-lactam n=69,079) during the study period. Retinal detachment repair procedures were performed within 365 days of the first prescription in 0.03% (95% confidence interval [CI] 0.01–0.06%) of the fluoroquinolone, 0.02% (95% CI 0.01–0.03%) of the macrolide, and 0.03% (95% CI 0.02–0.05%) of the β-lactam cohorts (p>0.05). Retinal detachment prophylaxis procedures for symptomatic retinal breaks were performed within 365 days of the first prescription in 0.01% (95% CI 0.00–0.03%) of the fluoroquinolone, 0.02% (95% CI 0.01–0.04%) of the macrolide, and 0

  1. The dark recovery rate in the photocycle of the bacterial photoreceptor YtvA is affected by the cellular environment and by hydration.

    Directory of Open Access Journals (Sweden)

    Francesca Pennacchietti

    Full Text Available We report thermal recovery kinetics of the lit state into the parental dark state, measured for the blue light-sensing photoreceptor YtvA inside overexpressing E. coli and B. subtilis bacterial cells, performed for the wild type and several mutated proteins. Recovery was followed as a recovery of the fluorescence, as this property is only found for the parental but not for the photochemically generated lit state. When cells were deposited onto a microscope glass plate, the observed thermal recovery rate in the photocycle was found ca. ten times faster in comparison to purified YtvA in solution. When the E. coli or B. subtilis colonies were soaked in an isotonic buffer, the dark relaxation became again much slower and was very similar to that observed for YtvA in solution. The observed effects show that rate constants can be tuned by the cellular environment through factors such as hydration.

  2. Bacterial vaginosis among women at high risk for HIV in Uganda: high rate of recurrent diagnosis despite treatment.

    Science.gov (United States)

    Francis, Suzanna C; Looker, Clare; Vandepitte, Judith; Bukenya, Justine; Mayanja, Yunia; Nakubulwa, Susan; Hughes, Peter; Hayes, Richard J; Weiss, Helen A; Grosskurth, Heiner

    2016-03-01

    Bacterial vaginosis (BV) is associated with increased risk for sexually transmitted infections (STIs) and HIV acquisition. This study describes the epidemiology of BV in a cohort of women at high risk for STI/HIV in Uganda over 2 years of follow-up between 2008-2011. 1027 sex workers or bar workers were enrolled and asked to attend 3-monthly follow-up visits. Factors associated with prevalent BV were analysed using multivariate random-effects logistic regression. The effect of treatment on subsequent episodes of BV was evaluated with survival analysis. Prevalences of BV and HIV at enrolment were 56% (573/1027) and 37% (382/1027), respectively. Overall, 905 (88%) women tested positive for BV at least once in the study, over a median of four visits. Younger age, a higher number of previous sexual partners and current alcohol use were independently associated with prevalent BV. BV was associated with STIs, including HIV. Hormonal contraception and condom use were protective against BV. Among 853 treated BV cases, 72% tested positive again within 3 months. There was no difference in time to subsequent BV diagnosis between treated and untreated women. BV was highly prevalent and persistent in this cohort despite treatment. More effective treatment strategies are urgently needed. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

  3. Risk of Retinal Detachment After Pediatric Cataract Surgery

    DEFF Research Database (Denmark)

    Haargaard, Birgitte; Andersen, Elisabeth W; Oudin, Anna;

    2014-01-01

    PURPOSE: To determine the long-term risk of retinal detachment following pediatric cataract surgery and to identify risk factors for retinal detachment. METHODS: We included all children (aged 0 to 17 years) who during the time period of 1977 to 2005 underwent pediatric cataract surgery in Denmark...... was based on medical chart review. RESULTS: Among 1043 eyes of 656 children undergoing surgery for pediatric cataract, 25 eyes (23 children) developed retinal detachment at a median time of 9.1 years after surgery. The overall 20-year risk of retinal detachment was 7% (95% confidence interval [CI]: 3...... (16% [95% CI: 6%-24%]). CONCLUSIONS: The estimated overall risk of retinal detachment 20 years after pediatric cataract surgery was 7%, but only 3% for isolated cataract. Particularly high risks of retinal detachment after cataract surgery were associated with mental retardation and having other...

  4. The ironic detachment of Edward Gibbon.

    Science.gov (United States)

    Trosman, Harry

    2009-06-01

    Edward Gibbon, the author of The History of the Decline and Fall of the Roman Empire, has been widely recognized as a master of irony. The historian's early life with parents he found self-serving and unreliable, his reaction to the events surrounding the death of his mother at the age of 9 and the decline of his father, left an impact on his personality and played a role in determining his choice of his life work. Irony has been approached from a psychoanalytic perspective as a mode of communication, as a stylistic device, as a modality through which one might view reality and as a way of uncovering the linkage between pretense and aspiration, between the apparent and the real. Gibbon's ironic detachment can be understood as rooted in his life history. He felt detached from his family of origin, in need of a protective device which would enable him to deal with passion. Sexual and aggressive impulses mobilized defensive postures that were later transformed into an attitude of skepticism and an interest in undercutting false beliefs and irrational authority, positions he attributes to religious ideation which served to instigate historical decline.

  5. A Sensitivity Analysis of SOLPS Plasma Detachment

    Science.gov (United States)

    Green, D. L.; Canik, J. M.; Eldon, D.; Meneghini, O.; AToM SciDAC Collaboration

    2016-10-01

    Predicting the scrape off layer plasma conditions required for the ITER plasma to achieve detachment is an important issue when considering divertor heat load management options that are compatible with desired core plasma operational scenarios. Given the complexity of the scrape off layer, such predictions often rely on an integrated model of plasma transport with many free parameters. However, the sensitivity of any given prediction to the choices made by the modeler is often overlooked due to the logistical difficulties in completing such a study. Here we utilize an OMFIT workflow to enable a sensitivity analysis of the midplane density at which detachment occurs within the SOLPS model. The workflow leverages the TaskFarmer technology developed at NERSC to launch many instances of the SOLPS integrated model in parallel to probe the high dimensional parameter space of SOLPS inputs. We examine both predictive and interpretive models where the plasma diffusion coefficients are chosen to match an empirical scaling for divertor heat flux width or experimental profiles respectively. This research used resources of the National Energy Research Scientific Computing Center, a DOE Office of Science User Facility, and is supported under Contracts DE-AC02-05CH11231, DE-AC05-00OR22725 and DE-SC0012656.

  6. Detachments of the retinal pigment epithelium at the posterior pole.

    Science.gov (United States)

    Noble, K G; Levitzky, M J; Carr, R E

    1976-08-01

    Multiple vitelliform cysts of the retina, a disorder of unknown cause in which there are multiple detachments of the retinal pigment epithelium at the posterior pole, occurred in five patients. In four patients all lesions were located outside the parafoveal area while one patient showed bilateral foveal elevations associated with more eccentric detachments. Several patients showed slow resolution of some of the detachments with mild disturbances of the pigment epithelium.

  7. Density threshold for plasma detachment in gas target

    Energy Technology Data Exchange (ETDEWEB)

    Ezumi, N. [Nagoya Univ. (Japan). Dept. of Energy Engineering and Science; Mori, S. [Department of Electrical Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-01 (Japan); Ohno, N. [Nagoya Univ. (Japan). Dept. of Energy Engineering and Science; Takagi, M. [Nagoya Univ. (Japan). Dept. of Energy Engineering and Science; Takamura, S. [Nagoya Univ. (Japan). Dept. of Energy Engineering and Science; Suzuki, H. [National Institute for Fusion Science, Nagoya 464-01 (Japan); Park, J. [Plasma Physics Laboratory, Princeton University, Princeton, NJ 08543 (United States)

    1997-02-01

    The simulated gas target divertor experiment has been performed to investigate the fundamental physics of plasma detachment in the linear plasma device, TPD-I, which has a high heat flux and high density plasma in steady state. The existence of a density threshold for plasma detachment was observed in our experiment. It is found that the electron-ion temperature relaxation process is a key to determine the density dependence of the plasma detachment. (orig.).

  8. Abrupt tectonics and rapid slab detachment with grain damage

    OpenAIRE

    2015-01-01

    Subduction zones are delineated by Earth’s ocean trenches, and are where tectonic plates sink into the mantle as cold heavy slabs, which in turn drive plate motion. But slabs can detach from their surface plates, thus altering tectonic driving forces. Slab detachment can occur if thick crust from continents or oceanic plateaux is swept by plate motion into the subduction zone, thus plugging it up. Detachment is also accelerated because mineral grains in the slab become smaller during deformat...

  9. Low-energy electron attachment and detachment in vibrationally excited oxygen

    Energy Technology Data Exchange (ETDEWEB)

    Aleksandrov, N L; Anokhin, E M, E-mail: nick_aleksandrov@mail.r, E-mail: nick_aleksandrov@hotmail.co [Moscow Institute of Physics and Technology, Dolgoprudny, 141700 (Russian Federation)

    2009-11-21

    Three-body electron attachment to O{sub 2} molecules and electron detachment from O{sub 2}{sup -} ions have been theoretically studied in vibrationally excited oxygen and O{sub 2}-containing mixtures. Assuming that electron attachment and detachment proceed via the formation of vibrationally excited temporary O{sub 2}{sup -} ions, the rates of these processes were determined on the basis of the statistical approach for the vibrational transfer and relaxation in collisions between O{sub 2}{sup -} ions and O{sub 2} molecules. The calculated attachment and detachment rate constants turned out to agree well with available measurements in unexcited oxygen. This method was extended to calculate attachment and detachment rates in vibrationally excited oxygen. It was shown that the effect of vibrational excitation on electron detachment is profound, whereas attachment of low-energy electrons to vibrationally excited O{sub 2} is inefficient. The calculated vibrational distribution of stable O{sub 2}{sup -} ions turned out to be non-equilibrium in an excited gas and the effective vibrational temperature of the ions was much lower than the vibrational temperature of molecules. An analytical method was suggested to determine this distribution and the effective vibrational temperature. The calculated rate constants were used to simulate the formation and decay of an electron-beam-generated plasma in N{sub 2} : O{sub 2} mixtures at elevated vibrational temperatures. The calculations showed that vibrational excitation of molecules leads to orders of magnitude increase in the plasma density and in the plasma lifetime, in agreement with available observations.

  10. Archaeal abundance across a pH gradient in an arable soil and its relationship to bacterial and fungal growth rates.

    Science.gov (United States)

    Bengtson, Per; Sterngren, Anna E; Rousk, Johannes

    2012-08-01

    Soil pH is one of the most influential factors for the composition of bacterial and fungal communities, but the influence of soil pH on the distribution and composition of soil archaeal communities has yet to be systematically addressed. The primary aim of this study was to determine how total archaeal abundance (quantitative PCR [qPCR]-based estimates of 16S rRNA gene copy numbers) is related to soil pH across a pH gradient (pH 4.0 to 8.3). Secondarily, we wanted to assess how archaeal abundance related to bacterial and fungal growth rates across the same pH gradient. We identified two distinct and opposite effects of pH on the archaeal abundance. In the lowest pH range (pH 4.0 to 4.7), the abundance of archaea did not seem to correspond to pH. Above this pH range, there was a sharp, almost 4-fold decrease in archaeal abundance, reaching a minimum at pH 5.1 to 5.2. The low abundance of archaeal 16S rRNA gene copy numbers at this pH range then sharply increased almost 150-fold with pH, resulting in an increase in the ratio between archaeal and bacterial copy numbers from a minimum of 0.002 to more than 0.07 at pH 8. The nonuniform archaeal response to pH could reflect variation in the archaeal community composition along the gradient, with some archaea adapted to acidic conditions and others to neutral to slightly alkaline conditions. This suggestion is reinforced by observations of contrasting outcomes of the (competitive) interactions between archaea, bacteria, and fungi toward the lower and higher ends of the examined pH gradient.

  11. Applicability of different hydraulic parameters to describe soil detachment in eroding rills.

    Science.gov (United States)

    Wirtz, Stefan; Seeger, Manuel; Zell, Andreas; Wagner, Christian; Wagner, Jean-Frank; Ries, Johannes B

    2013-01-01

    This study presents the comparison of experimental results with assumptions used in numerical models. The aim of the field experiments is to test the linear relationship between different hydraulic parameters and soil detachment. For example correlations between shear stress, unit length shear force, stream power, unit stream power and effective stream power and the detachment rate does not reveal a single parameter which consistently displays the best correlation. More importantly, the best fit does not only vary from one experiment to another, but even between distinct measurement points. Different processes in rill erosion are responsible for the changing correlations. However, not all these procedures are considered in soil erosion models. Hence, hydraulic parameters alone are not sufficient to predict detachment rates. They predict the fluvial incising in the rill's bottom, but the main sediment sources are not considered sufficiently in its equations. The results of this study show that there is still a lack of understanding of the physical processes underlying soil erosion. Exerted forces, soil stability and its expression, the abstraction of the detachment and transport processes in shallow flowing water remain still subject of unclear description and dependence.

  12. Scleral buckle is good option for treatment of uncomplicated retinal detachment

    Directory of Open Access Journals (Sweden)

    Iuuki Takasaka

    2012-12-01

    Full Text Available OBJETIVE: To describe the reattachment rate and visual acuity results of patients with uncomplicated rhegmatogenous retinal detachment who underwent segmental scleral buckle surgery. METHODS: Prospective case series of 100 patients with visual loss or symptoms (floaters and photopsia of less than 30 days' duration scheduled for surgery. No patient had a retinal break greater than 30°, a retinal detachment larger than 2 quadrants or proliferative vitreoretinopathy. RESULTS: The 1-week, 1-month, and 6-month anatomical success rates were 93%, 100%, and 100%, respectively. Seven patients underwent one additional retinal detachment surgery (pars plan vitrectomy after primary failure at 1-week follow-up. The preoperative, 1-month, and 6-month best correct visual acuity were 20/100, 20/80, and 20/50, respectively. The postoperative complications were: eyelid edema in 10% of the patients, transient ocular hypertension in 5%, macular pucker in 3%, transient diplopia in 3%, and hyphema (<0.5mm in 1%. CONCLUSION: In patients with uncomplicated retinal detachment, segmental scleral buckle showed a very good anatomical and functional success, with a few number of major complications.

  13. Differential response of planktonic primary, bacterial, and dimethylsulfide production rates to static vs. dynamic light exposure in upper mixed-layer summer sea waters

    Science.gov (United States)

    Galí, M.; Simó, R.; Pérez, G. L.; Ruiz-González, C.; Sarmento, H.; Royer, S.-J.; Fuentes-Lema, A.; Gasol, J. M.

    2013-12-01

    Microbial plankton experience short-term fluctuations in total solar irradiance and in its spectral composition as they are vertically moved by turbulence in the oceanic upper mixed layer (UML). The fact that the light exposure is not static but dynamic may have important consequences for biogeochemical processes and ocean-atmosphere fluxes. However, most biogeochemical processes other than primary production, like bacterial production or dimethylsulfide (DMS) production, are seldom measured in sunlight and even less often in dynamic light fields. We conducted four experiments in oligotrophic summer stratified Mediterranean waters, where a sample from the UML was incubated in ultraviolet (UV)-transparent bottles at three fixed depths within the UML and on a vertically moving basket across the same depth range. We assessed the response of the phyto- and bacterioplankton community with physiological indicators based on flow cytometry singe-cell measurements, fast repetition rate fluorometry (FRRf), phytoplankton pigment concentrations and particulate light absorption. Dynamic light exposure caused a subtle disruption of the photoinhibition and photoacclimation processes associated with ultraviolet radiation (UVR), which slightly alleviated bacterial photoinhibition but did not favor primary production. Gross DMS production (GPDMS) decreased sharply with depth in parallel to shortwave UVR, and displayed a dose-dependent response that mixing did not significantly disrupt. To our knowledge, we provide the first measurements of GPDMS under in situ UV-inclusive optical conditions.

  14. Differential response of planktonic primary, bacterial, and dimethylsulfide production rates to vertically-moving and static incubations in upper mixed-layer summer sea waters

    Directory of Open Access Journals (Sweden)

    M. Galí

    2013-05-01

    Full Text Available Microbial plankton experience fluctuations in total solar irradiance and in its spectral composition as they are vertically moved by turbulence in the oceanic upper mixed layer (UML. The fact that the light exposure is not static but dynamic may have important consequences for biogeochemical processes and ocean-atmosphere fluxes. However, most biogeochemical processes other than primary production, like bacterial production or dimethylsulfide (DMS production, are seldom measured in sunlight and even less often in dynamic light fields. We conducted four experiments in oligotrophic summer stratified Mediterranean waters, where a sample from the UML was incubated in ultraviolet (UV-transparent bottles at three fixed depths within the UML and on a vertically-moving basket across the same depth range. We assessed the response of the phyto- and bacterioplankton community with physiological indicators based on flow cytometry singe-cell measurements, Fast Repetition Rate fluorometry (FRRf, phytoplankton pigment concentrations and particulate light absorption. Dynamic light exposure caused a disruption of the photoinhibition and photoacclimation processes associated to ultraviolet radiation (UVR, which slightly alleviated bacterial photoinhibition but did not favor primary production. Gross DMS production (GPDMS decreased sharply with depth in parallel to shortwave UVR, and displayed a dose-dependent response that mixing did not significantly disrupt. To our knowledge, we provide the first measurements of GPDMS under in situ UV-inclusive optical conditions.

  15. Differential response of planktonic primary, bacterial, and dimethylsulfide production rates to vertically-moving and static incubations in upper mixed-layer summer sea waters

    Science.gov (United States)

    Galí, M.; Simó, R.; Pérez, G. L.; Ruiz-González, C.; Sarmento, H.; Royer, S.-J.; Fuentes-Lema, A.; Gasol, J. M.

    2013-05-01

    Microbial plankton experience fluctuations in total solar irradiance and in its spectral composition as they are vertically moved by turbulence in the oceanic upper mixed layer (UML). The fact that the light exposure is not static but dynamic may have important consequences for biogeochemical processes and ocean-atmosphere fluxes. However, most biogeochemical processes other than primary production, like bacterial production or dimethylsulfide (DMS) production, are seldom measured in sunlight and even less often in dynamic light fields. We conducted four experiments in oligotrophic summer stratified Mediterranean waters, where a sample from the UML was incubated in ultraviolet (UV)-transparent bottles at three fixed depths within the UML and on a vertically-moving basket across the same depth range. We assessed the response of the phyto- and bacterioplankton community with physiological indicators based on flow cytometry singe-cell measurements, Fast Repetition Rate fluorometry (FRRf), phytoplankton pigment concentrations and particulate light absorption. Dynamic light exposure caused a disruption of the photoinhibition and photoacclimation processes associated to ultraviolet radiation (UVR), which slightly alleviated bacterial photoinhibition but did not favor primary production. Gross DMS production (GPDMS) decreased sharply with depth in parallel to shortwave UVR, and displayed a dose-dependent response that mixing did not significantly disrupt. To our knowledge, we provide the first measurements of GPDMS under in situ UV-inclusive optical conditions.

  16. Using random walk in models specified by stochastic differential equations to determine the best expression for the bacterial growth rate

    DEFF Research Database (Denmark)

    Optical Density bioscreen measurements of P. aeruginosa to perform a Maximum Likelihood estimation of the model parameters and subsequently obtain a smoothing estimate for the model state variables by means of a nonlinear smoothing algorithm based on the extended Kalman filter, using an implementation....... aeruginosa we observe a growth pattern far from Monod growth. Therefore a reformulation of the growth expression is necessary. Without any pre-knowledge about the functional dependence between the growth rate and the substrate content and with only limited experimental resources necessary, the proposed...... described by Kristensen et. al [2]. The resulting time series allows us graphically to inspect the functional dependence of the growth rate on the substrate content. From the method described above we find three new plausible expressions for μ(S). Therefore we apply the likelihood-ratio test to compare...

  17. The effects of temperature and growth rate on the proportion of unsaturated fatty acids in bacterial lipids.

    Science.gov (United States)

    Gill, C O; Suisted, J R

    1978-01-01

    The effects of temperature and growth rate on the fatty acid composition of the extractable lipids of four mesophilic and three psychotrophic bacteria were examined. Two of the mesophiles (Escherichia coli and Pseudomonas aeruginosa) increased the proportion of unsaturated fatty acids in their lipids with decreasing temperature over their whole growth temperature range. The other mesophiles (Enterobacter aerogenes and Lactobacillus casei) increased the proportion of unsaturated fatty acids with decreasing temperature only over the lower half of their growth temperature ranges. The psychrotrophs Pseudomonas fluorescens and Enterobacter sp. had a constant proportion of unsaturated acids over the lower half of their growth temperature range, while the psychotrophic Lactobacillus sp. showed no consistent change in its unsaturated fatty acid composition with temperature. All species showed some variation of unsaturated fatty acid composition with growth rate at the highest and lowest growth temperatures, although such variations were small in some species (Ent. aerogenes and Lactobacillus sp.).

  18. Crystal Shape Evolution in Detached Bridgman Growth

    Science.gov (United States)

    Volz, M. P.; Mazuruk, K.

    2013-01-01

    Detached (or dewetted) Bridgman crystal growth defines that process in which a gap exists between a growing crystal and the crucible wall. Existence of the gap provides several advantages, including no sticking of the crystal to the crucible wall, reduced thermal and mechanical stresses, reduced dislocations, and no heterogeneous nucleation by the crucible. Numerical calculations are used to determine the conditions in which a gap can exist. According to crystal shape stability theory, only some of these gap widths will be dynamically stable. Beginning with a crystal diameter that differs from stable conditions, the transient crystal growth process is analyzed. In microgravity, dynamic stability depends only on capillary effects and is decoupled from heat transfer. Depending on the initial conditions and growth parameters, the crystal shape will evolve towards the crucible wall, towards a stable gap width, or towards the center of the crucible, collapsing the meniscus. The effect of a tapered crucible on dynamic stability is also described.

  19. Maritime Training Serbian Autonomous Vessel Protection Detachment

    Directory of Open Access Journals (Sweden)

    Šoškić Svetislav D.

    2014-06-01

    Full Text Available The crisis in Somalia has caused appearance of piracy at sea in the Gulf of Aden and the Western Indian Ocean. Somali pirates have become a threat to economic security of the world because almost 30 percent of world oil and 20 percent of global trade passes through the Gulf of Aden. Solving the problem of piracy in this part of the world have included international organizations, institutions, military alliances and the states, acting in accordance with international law and UN Security Council resolutions. The European Union will demonstrate the application of a comprehensive approach to solving the problem of piracy at sea and the crisis in Somalia conducting naval operation — EU NAVFOR Atalanta and operation EUTM under the Common Security and Defense Policy. The paper discusses approaches to solving the problem of piracy in the Gulf of Aden and the crisis in Somalia. Also, the paper points to the complexity of the crisis in Somalia and dilemmas correctness principles that are applied to solve the problem piracy at sea. One of goals is protections of vessels of the World Food Programme (WFP delivering food aid to displaced persons in Somalia. Republic of Serbia joined in this mission and trained and sent one a autonomous team in this military operation for protection WFP. This paper consist the problem of modern piracy, particularly in the area of the Horn of Africa became a real threat for the safety of maritime ships and educational process of Serbian Autonomous vessel protection detachment. Serbian Military Academy adopted and developed educational a training program against piracy applying all the provisions and recommendations of the IMO conventions and IMO model courses for Serbian Autonomous vessel protection detachment.

  20. Recurrent Annular Peripheral Choroidal Detachment after Trabeculectomy

    Science.gov (United States)

    Liu, Shaohui; Sun, Lisa L.; Kavanaugh, A. Scott; Langford, Marlyn P.; Liang, Chanping

    2013-01-01

    We report a challenging case of recurrent flat anterior chamber without hypotony after trabeculectomy in a 54-year-old Black male with a remote history of steroid-treated polymyositis, cataract surgery, and uncontrolled open angle glaucoma. The patient presented with a flat chamber on postoperative day 11, but had a normal fundus exam and intraocular pressure (IOP). Flat chamber persisted despite treatment with cycloplegics, steroids, and a Healon injection into the anterior chamber. A transverse B-scan of the peripheral fundus revealed a shallow annular peripheral choroidal detachment. The suprachoroidal fluid was drained. The patient presented 3 days later with a recurrent flat chamber and an annular peripheral choroidal effusion. The fluid was removed and reinforcement of the scleral flap was performed with the resolution of the flat anterior chamber. A large corneal epithelial defect developed after the second drainage. The oral prednisone was tapered quickly and the topical steroid was decreased. One week later, his vision decreased to count fingers with severe corneal stromal edema and Descemet's membrane folds that improved to 20/50 within 24 h of resumption of the oral steroid and frequent topical steroid. The patient's visual acuity improved to 20/20 following a slow withdrawal of the oral and topical steroid. Eight months after surgery, the IOP was 15 mm Hg without glaucoma medication. The detection of a shallow anterior choroidal detachment by transverse B-scan is critical to making the correct diagnosis. Severe cornea edema can occur if the steroid is withdrawn too quickly. Thus, steroids should be tapered cautiously in steroid-dependent patients. PMID:24348402

  1. Recurrent Annular Peripheral Choroidal Detachment after Trabeculectomy

    Directory of Open Access Journals (Sweden)

    Shaohui Liu

    2013-10-01

    Full Text Available We report a challenging case of recurrent flat anterior chamber without hypotony after trabeculectomy in a 54-year-old Black male with a remote history of steroid-treated polymyositis, cataract surgery, and uncontrolled open angle glaucoma. The patient presented with a flat chamber on postoperative day 11, but had a normal fundus exam and intraocular pressure (IOP. Flat chamber persisted despite treatment with cycloplegics, steroids, and a Healon injection into the anterior chamber. A transverse B-scan of the peripheral fundus revealed a shallow annular peripheral choroidal detachment. The suprachoroidal fluid was drained. The patient presented 3 days later with a recurrent flat chamber and an annular peripheral choroidal effusion. The fluid was removed and reinforcement of the scleral flap was performed with the resolution of the flat anterior chamber. A large corneal epithelial defect developed after the second drainage. The oral prednisone was tapered quickly and the topical steroid was decreased. One week later, his vision decreased to count fingers with severe corneal stromal edema and Descemet's membrane folds that improved to 20/50 within 24 h of resumption of the oral steroid and frequent topical steroid. The patient's visual acuity improved to 20/20 following a slow withdrawal of the oral and topical steroid. Eight months after surgery, the IOP was 15 mm Hg without glaucoma medication. The detection of a shallow anterior choroidal detachment by transverse B-scan is critical to making the correct diagnosis. Severe cornea edema can occur if the steroid is withdrawn too quickly. Thus, steroids should be tapered cautiously in steroid-dependent patients.

  2. In Vivo Synthesis and Turnover of α-Amylase in Attached and Detached Cotyledons of Vigna mungo Seeds 1

    Science.gov (United States)

    Koshiba, Tomokazu; Minamikawa, Takao

    1983-01-01

    α-Amylase activity increased in attached cotyledons of germinated Vigna mungo seeds until the 5th day after imbibition and decreased thereafter, whereas in detached and incubated cotyledons the activity continuously increased and, at the 6th day, reached the value more than three times that of the maximum activity of attached cotyledons. Zymograms of the activities and Ouchterlony double immunodiffusion test on the activities of attached and detached cotyledons showed that the increase of activity in detached cotyledons was due to the identical enzyme as in attached tissues. α-Amylase contents, determined by single radial immunodiffusion method, changed in parallel with enzyme activity in both attached and detached cotyledons, which also suggested the de novo synthesis of α-amylase in V. mungo cotyledons. The rate of incorporation of the label from [3H]leucine into α-amylase and the ratios of dpm in α-amylase/dpm in trichloroacetic acid-insoluble fraction did not show significant difference between attached and detached cotyledons. The results indicated that in attached cotyledons fluctuation of α-amylase activity was regulated by both synthesis and degradation of the enzyme, whereas in detached cotyledons α-amylase was synthesized and accumulated, because of low degrading activity during incubation. Images Fig. 1 Fig. 2 Fig. 4 PMID:16662780

  3. In Vivo Synthesis and Turnover of alpha-Amylase in Attached and Detached Cotyledons of Vigna mungo Seeds.

    Science.gov (United States)

    Koshiba, T; Minamikawa, T

    1983-01-01

    alpha-Amylase activity increased in attached cotyledons of germinated Vigna mungo seeds until the 5th day after imbibition and decreased thereafter, whereas in detached and incubated cotyledons the activity continuously increased and, at the 6th day, reached the value more than three times that of the maximum activity of attached cotyledons. Zymograms of the activities and Ouchterlony double immunodiffusion test on the activities of attached and detached cotyledons showed that the increase of activity in detached cotyledons was due to the identical enzyme as in attached tissues. alpha-Amylase contents, determined by single radial immunodiffusion method, changed in parallel with enzyme activity in both attached and detached cotyledons, which also suggested the de novo synthesis of alpha-amylase in V. mungo cotyledons.The rate of incorporation of the label from [(3)H]leucine into alpha-amylase and the ratios of dpm in alpha-amylase/dpm in trichloroacetic acid-insoluble fraction did not show significant difference between attached and detached cotyledons. The results indicated that in attached cotyledons fluctuation of alpha-amylase activity was regulated by both synthesis and degradation of the enzyme, whereas in detached cotyledons alpha-amylase was synthesized and accumulated, because of low degrading activity during incubation.

  4. Return to Work and Risk of Subsequent Detachment From Employment After Myocardial Infarction: Insights From Danish Nationwide Registries.

    Science.gov (United States)

    Smedegaard, Laerke; Numé, Anna-Karin; Charlot, Mette; Kragholm, Kristian; Gislason, Gunnar; Hansen, Peter R

    2017-10-04

    Limited data are available on return to work and subsequent detachment from employment after admission for myocardial infarction (MI). Using individual-level linkage of data from nationwide registries, we identified patients of working age (30-65 years) discharged after first-time MI in the period 1997 to 2012, who were employed before admission. To assess the cumulative incidence of return to work and detachment from employment, the Aalen Johansen estimator was used. Incidences were compared with population controls matched on age and sex. Logistic regression was applied to estimate odds ratios for associations between detachment from employment and age, sex, comorbidities, income, and education level. Of 39 296 patients of working age discharged after first-time MI, 22 394 (56.9%) were employed before admission. Within 1 year 91.1% (95% confidence interval [CI], 90.7%-91.5%) of subjects had returned to work, but 1 year after their return 24.2% (95% CI, 23.6%-24.8%) were detached from employment and received social benefits. Detachment rates were highest in patients aged 60 to 65 and 30 to 39 years, and significantly higher in patients with MI compared with population controls. Predictors of detachment were heart failure (odds ratio 1.20 [95% CI, 1.08-1.34]), diabetes mellitus (odds ratio 1.13 [95% CI, 1.01-1.25]), and depression (odds ratio 1.77 [95% CI, 1.55-2.01]). High education level and high income favored continued employment. Despite that most patients returned to work after first-time MI, about 1 in 4 was detached from employment after 1 year. Several factors including age and lower socioeconomic status were associated with risk of detachment from employment. © 2017 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

  5. Tamponade in surgery for retinal detachment associated with proliferative vitreoretinopathy

    Science.gov (United States)

    Schwartz, Stephen G; Flynn, Harry W; Lee, Wen-Hsiang; Wang, Xue

    2014-01-01

    Background Retinal detachment (RD) with proliferative vitreoretinopathy (PVR) often requires surgery to restore normal anatomy and to stabilize or improve vision. PVR usually occurs in association with recurrent RD (that is, after initial retinal re-attachment surgery) but occasionally may be associated with primary RD. Either way, a tamponade agent (gas or silicone oil) is needed during surgery to reduce the rate of postoperative recurrent RD. Objectives The objective of this review was to assess the relative safety and effectiveness of various tamponade agents used with surgery for retinal detachment (RD) complicated by proliferative vitreoretinopathy (PVR). Search methods We searched CENTRAL (which contains the Cochrane Eyes and Vision Group Trials Register) (The Cochrane Library 2013, Issue 5), Ovid MEDLINE, Ovid MEDLINE In-Process and Other Non-Indexed Citations, Ovid MEDLINE Daily, Ovid OLDMEDLINE (January 1946 to June 2013), EMBASE (January 1980 to June 2013), Latin American and Caribbean Literature on Health Sciences (LILACS) (January 1982 to June 2013), the metaRegister of Controlled Trials (mRCT) (www.controlled-trials.com), ClinicalTrials.gov (www.clinicaltrials.gov) and the WHO International Clinical Trials Registry Platform (ICTRP) (www.who.int/ictrp/search/en). We did not use any date or language restrictions in the electronic searches for trials. We last searched the electronic databases on 26 June 2013. Selection criteria We included randomized controlled trials (RCTs) of participants undergoing surgery for RD associated with PVR that compared various tamponade agents. Data collection and analysis Two review authors screened the search results independently. We used the standard methodological procedures expected by The Cochrane Collaboration. PMID:24532038

  6. DETACHMENT OF HUMAN FIBROBLASTS FROM FEP-TEFLON SURFACES

    NARCIS (Netherlands)

    VANKOOTEN, TG; SCHAKENRAAD, JM; VANDERMEI, HC; BUSSCHER, HJ

    1991-01-01

    In this study a comparison is made between the detachment behavior of human fibroblasts adhered to hydrophobic FEP-Teflon (water contact angle 109 degrees) and to hydrophilic glass (water contact angle smaller than 15 degrees) during exposure to a laminar, incrementally loaded flow. Detachment from

  7. Cell detachment method using shock-wave–induced cavitation

    NARCIS (Netherlands)

    Junge, L.; Ohl, C.D.; Wolfrum, B.; Arora, M.; Ikink, R.

    2003-01-01

    The detachment of adherent HeLa cells from a substrate after the interaction with a shock wave is analyzed. Cavitation bubbles are formed in the trailing, negative pressure cycle following the shock front. We find that the regions of cell detachment are strongly correlated with spatial presence of c

  8. Recalcitrance of Streptococcus mutans biofilms towards detergent-stimulated detachment

    NARCIS (Netherlands)

    Landa, AS; van de Belt-Gritter, B; van der Mei, HC; Busscher, HJ

    1999-01-01

    The biofilm mode of growth protects the plaque microorganisms against environmental attacks, such as from antimicrobials or detergents. Detergents have a demonstrated ability to detach initially adhering bacteria from enamel surfaces, but the ability of detergent components to detach plaque bacteria

  9. Effect of aeration rate on performance and stability of algal-bacterial symbiosis system to treat domestic wastewater in sequencing batch reactors.

    Science.gov (United States)

    Tang, Cong-Cong; Zuo, Wei; Tian, Yu; Sun, Ni; Wang, Zhen-Wei; Zhang, Jun

    2016-12-01

    This study investigated aeration rate (0, 0.2, 0.4 and 1.0L/min) effects on algal-bacterial symbiosis (ABS) and conventional activated sludge (CAS) systems while treating domestic wastewater in sequencing batch reactors. Experiment results showed that ABS system performed better on NH4(+)-N, total nitrogen and total phosphorus removal than CAS system, especially under lower aeration rate condition (0.2Lair/min), with removal efficiencies improvements of 18.90%, 12.45% and 46.66%, respectively. The mechanism study demonstrated that a favorable aeration rate reduction (half of traditional value in CAS system) could enhance algae growth but weaken hydraulic shear force, which contributed to the interactions between algae and sludge flocs and further stability of ABS system. In addition, algae growth protected both ammonia and nitrite oxidizing bacteria from optical damage. It is expected that the present study would provide some new insights into ABS system and be helpful for development of low-energy demand wastewater treatment process. Copyright © 2016 Elsevier Ltd. All rights reserved.

  10. Changes in performance and bacterial communities in response to various process disturbances in a high-rate biohydrogen reactor fed with galactose.

    Science.gov (United States)

    Park, Jeong-Hoon; Kumar, Gopalakrishnan; Park, Jong-Hun; Park, Hee-Deung; Kim, Sang-Hyoun

    2015-01-01

    High-rate biohydrogen production was achieved via hybrid immobilized cells fed with galactose in a continuous reactor system. The hybrid immobilized cells were broken down after 20 days and began to form granules by self-aggregation. The peak hydrogen production rate (HPR) and hydrogen yield (HY) of 11.8 ± 0.6 LH2/L-d and 2.1 ± 0.1 mol H2/molgalactose(added), respectively, were achieved at the hydraulic retention time (HRT) of 8h with an organic loading rate (OLR) of 45 g/L-d. This is the highest yet reported for the employment of galactose in a continuous system. Various process disturbances including shock loading, acidification, alkalization and starvation were examined through bacterial community analysis via pyrosequencing of the 16S rRNA genes. The proportion of Clostridia increased during the stable biohydrogen production periods, while that of Bacilli increased when the reactor was disturbed. However, due to the stability of the self-aggregated granules, the process performance was regained within 4-7 days.

  11. Ocular hemodynamics in patients with rhegmatogenous retinal detachment

    Directory of Open Access Journals (Sweden)

    N. H. Zavgorodnya

    2014-10-01

    Full Text Available Aim. In case of retinal detachment atrophic processes lead to irreversible loss of functions within 4–6 days, it happens on underlying low ocular blood flow. In order to evaluate the degree of violation of regional hemodynamics in patients with retinal detachment two groups of patients were examined: the main group (52 patients with rhegmatogenous retinal detachment and the control group (24 myopic patients with lattice form of peripheral chorioretinal dystrophy. Methods and results. Doppler and reography results had been compared, significant decrease of blood flow in patients with retinal detachment was found. No differences between affected and fellow eye in these patients, close negative correlation between the level of ocular blood flow and the degree of myopia in the control group. Conclusion. This demonstrates the feasibility of actions to improve regional blood flow in patients operated on for retinal detachment.

  12. Droplet detachment by air flow for microstructured superhydrophobic surfaces.

    Science.gov (United States)

    Hao, Pengfei; Lv, Cunjing; Yao, Zhaohui

    2013-04-30

    Quantitative correlation between critical air velocity and roughness of microstructured surface has still not been established systematically until the present; the dynamics of water droplet detachment by air flow from micropillar-like superhydrophobic surfaces is investigated by combining experiments and simulation comparisons. Experimental evidence demonstrates that the onset of water droplet detachment from horizontal micropillar-like superhydrophobic surfaces under air flow always starts with detachment of the rear contact lines of the droplets from the pillar tops, which exhibits a similar dynamic mechanism for water droplet motion under a gravity field. On the basis of theoretical analysis and numerical simulation, an explicit analytical model is proposed for investigating the detaching mechanism, in which the critical air velocity can be fully determined by several intrinsic parameters: water-solid interface area fraction, droplet volume, and Young's contact angle. This model gives predictions of the critical detachment velocity of air flow that agree well with the experimental measurements.

  13. Spontaneous Solitaire™ AB Thrombectomy Stent Detachment During Stroke Treatment

    Energy Technology Data Exchange (ETDEWEB)

    Akpinar, Suha, E-mail: akpinarsuha@hotmail.com; Yilmaz, Guliz, E-mail: glz.yilmaz@hotmail.com [Near East University Hospital, Department of Radiology, Faculty of Medicine (Turkey)

    2015-04-15

    Spontaneous Solitaire™ stent retriever detachment is a rarely defined entity seen during stroke treatment, which can result in a disastrous clinical outcome if it cannot be solved within a critical stroke treatment time window. Two solutions to this problem are presented in the literature. The first is to leave the stent in place and apply angioplasty to the detached stent, while the second involves surgically removing the stent from the location at which it detached. Here, we present a case of inadvertent stent detachment during stroke treatment for a middle cerebral artery M1 occlusion resulting in progressive thrombosis. The detached stent was removed endovascularly by another Solitaire stent, resulting in the recanalization of the occluded middle cerebral artery.

  14. Tracing detached and attached care practices in nursing education

    DEFF Research Database (Denmark)

    Soffer, Ann Katrine B.

    2014-01-01

    of care are not explicated in the curriculum or textbooks; however, they surfaced once this crooked approach to studying care in a simulated practice was applied. The article starts from the assertion that detached engagements are not recognized within the field of nursing education as an equal component...... to attachments. Yet empirical cases from the skills lab and hospitals illustrate how students sometimes felt emotionally attached to plastic dummies and how experienced nurses sometimes practised a degree of detachment in relation to human patients. Detached engagements will therefore be presented as part...... of care practices of nurses rendering the ability to detach in engagement with patients a professional skill that students also need to learn. In the analysis to follow, attached and detached engagements are located on an equal plane by integrating both in to the same conceptual framework, rather than...

  15. Interkingdom Responses to Bacterial Quorum Sensing Signals Regulate Frequency and Rate of Nodulation in Legume-Rhizobia Symbiosis.

    Science.gov (United States)

    Palmer, Andrew G; Mukherjee, Arijit; Stacy, Danielle M; Lazar, Stephen; Ané, Jean-Michel; Blackwell, Helen E

    2016-11-17

    Density-dependent phenotypic switching in bacteria, the phenomenon of quorum sensing (QS), is instrumental in many pathogenic and mutualistic behaviors. In many Gram-negative bacteria, QS is regulated by N-acylated-l-homoserine lactones (AHLs). Synthetic analogues of these AHLs hold significant promise for regulating QS at the host-symbiont interface. Regulation depends on refined temporal and spatial models of quorums under native conditions. Critical to this is an understanding of how the presence of these signals may affect a prospective host. We screened a library of AHL analogues for their ability to regulate the legume-rhizobia mutualistic symbiosis (nodulation) between Medicago truncatula and Sinorhizobium meliloti. Using an established QS-reporter line of S. meliloti and nodulation assays with wild-type bacteria, we identified compounds capable of increasing either the rate of nodule formation or total nodule number. Most importantly, we identified compounds with activity exclusive to either host or pathogen, underscoring the potential to generate QS modulators selective to bacteria with limited effects on a prospective host.

  16. Exfoliation rate of mammary epithelial cells in milk on bovine mastitis caused by Staphylococcus aureus is associated with bacterial load.

    Science.gov (United States)

    Nagasawa, Yuya; Kiku, Yoshio; Sugawara, Kazue; Tanabe, Fuyuko; Hayashi, Tomohito

    2017-09-11

    The exfoliation rate of mammary epithelial cells (MECs) in milk is affected by physiological, breeding and environmental factors. Little is known about the relationship between the MEC exfoliation into milk and mammary-infected Staphylococcus aureus (S. aureus) load on bovine mastitis caused by S. aureus. The aim of this study was to investigate the relationship between S. aureus load and the proportion of MEC exfoliation in milk using five substantial bovine mastitis models. In 64 randomly extracted milk samples from udders at 3-21 days after S. aureus infusion, there were various samples with different numbers of S. aureus counts and somatic cell counts. No significant correlations were found between the S. aureus counts and somatic cell count (r = 0.338). In contrast, a significant correlation was noted between S. aureus counts and the proportion of cytokeratin-positive cells in the milk from the infused udders (r = 0.734, P S. aureus may contribute to reduced milk yield. © 2017 Japanese Society of Animal Science.

  17. Bacterial cell curvature through mechanical control of cell growth

    DEFF Research Database (Denmark)

    Cabeen, M.; Charbon, Godefroid; Vollmer, W.

    2009-01-01

    The cytoskeleton is a key regulator of cell morphogenesis. Crescentin, a bacterial intermediate filament-like protein, is required for the curved shape of Caulobacter crescentus and localizes to the inner cell curvature. Here, we show that crescentin forms a single filamentous structure...... that collapses into a helix when detached from the cell membrane, suggesting that it is normally maintained in a stretched configuration. Crescentin causes an elongation rate gradient around the circumference of the sidewall, creating a longitudinal cell length differential and hence curvature. Such curvature...... can be produced by physical force alone when cells are grown in circular microchambers. Production of crescentin in Escherichia coli is sufficient to generate cell curvature. Our data argue for a model in which physical strain borne by the crescentin structure anisotropically alters the kinetics...

  18. Modeling soil detachment capacity by rill flow using hydraulic parameters

    Science.gov (United States)

    Wang, Dongdong; Wang, Zhanli; Shen, Nan; Chen, Hao

    2016-04-01

    The relationship between soil detachment capacity (Dc) by rill flow and hydraulic parameters (e.g., flow velocity, shear stress, unit stream power, stream power, and unit energy) at low flow rates is investigated to establish an accurate experimental model. Experiments are conducted using a 4 × 0.1 m rill hydraulic flume with a constant artificial roughness on the flume bed. The flow rates range from 0.22 × 10-3 m2 s-1 to 0.67 × 10-3 m2 s-1, and the slope gradients vary from 15.8% to 38.4%. Regression analysis indicates that the Dc by rill flow can be predicted using the linear equations of flow velocity, stream power, unit stream power, and unit energy. Dc by rill flow that is fitted to shear stress can be predicted with a power function equation. Predictions based on flow velocity, unit energy, and stream power are powerful, but those based on shear stress, especially on unit stream power, are relatively poor. The prediction based on flow velocity provides the best estimates of Dc by rill flow because of the simplicity and availability of its measurements. Owing to error in measuring flow velocity at low flow rates, the predictive abilities of Dc by rill flow using all hydraulic parameters are relatively lower in this study compared with the results of previous research. The measuring accuracy of experiments for flow velocity should be improved in future research.

  19. Detachment of liquid droplets from fibres--experimental and theoretical evaluation of detachment force due to interfacial tension effects.

    Science.gov (United States)

    Mullins, Benjamin J; Pfrang, Andreas; Braddock, Roger D; Schimmel, Thomas; Kasper, Gerhard

    2007-08-15

    The detachment of barrel-shaped oil droplets from metal, glass and polymer fibres was examined using an atomic force microscope (AFM). The AFM was used to detach the droplets from the fibres while measuring the force-distance relationship. A novel fibre-droplet interfacial tension model was applied to predict the force required to draw the droplet away from its preferential axisymmetric position on the fibre, and also to predict the maximal force required to detach the droplet. The model assumes that the droplet retains a spherical shape during detachment, i.e., that droplet distortion is negligible. This assumption was found to be reasonably accurate for small radius oil droplets (droplets (>25 microm). However, it was found that the model produced a good agreement with the maximal detachment force measured experimentally--regardless of droplet size and degree of deformation--even though the model could not predict droplet extension beyond a length of one droplet radius.

  20. Advantages of diabetic tractional retinal detachment repair

    Directory of Open Access Journals (Sweden)

    Sternfeld A

    2015-10-01

    Full Text Available Amir Sternfeld, Ruth Axer-Siegel, Hadas Stiebel-Kalish, Dov Weinberger, Rita Ehrlich Department of Ophthalmology, Rabin Medical Center, Beilinson Campus, Petach Tikva, Israel Purpose: To evaluate the outcomes and complications of patients with diabetic tractional retinal detachment (TRD treated with pars plana vitrectomy (PPV.Patients and methods: We retrospectively studied a case series of 24 eyes of 21 patients at a single tertiary, university-affiliated medical center. A review was carried out on patients who underwent PPV for the management of TRD due to proliferative diabetic retinopathy from October 2011 to November 2013. Preoperative and final visual outcomes, intraoperative and postoperative complications, and medical background were evaluated.Results: A 23 G instrumentation was used in 23 eyes (95.8%, and a 25 G instrumentation in one (4.2%. Mean postoperative follow-up time was 13.3 months (4–30 months. Visual acuity significantly improved from logarithm of the minimum angle of resolution (LogMAR 1.48 to LogMAR 1.05 (P<0.05. Visual acuity improved by ≥3 lines in 75% of patients. Intraoperative complications included iatrogenic retinal breaks in seven eyes (22.9% and vitreal hemorrhage in nine eyes (37.5%. In two eyes, one sclerotomy was enlarged to 20 G (8.3%. Postoperative complications included reoperation in five eyes (20.8% due to persistent subretinal fluid (n=3, vitreous hemorrhage (n=1, and dislocated intraocular lens (n=1. Thirteen patients (54.2% had postoperative vitreous hemorrhage that cleared spontaneously, five patients (20.8% required antiglaucoma medications for increased intraocular pressure, seven patients (29.2% developed an epiretinal membrane, and two patients (8.3% developed a macular hole.Conclusion: Patients with diabetic TRD can benefit from PPV surgery. Intraoperative and postoperative complications can be attributed to the complexity of this disease. Keywords: diabetic retinopathy, proliferative

  1. Young Detached Triple System LT CMa

    CERN Document Server

    Bakis, V; Bilir, S; Bakis, H; Demircan, O; Hensberge, H

    2010-01-01

    New high resolution spectra of the short period (P~1.76 days) young detached binary LT CMa are reported for the first time. By combining the results from the analysis of new radial velocity curves and published light curves, we determine values for the masses, radii and temperatures as follows: M_1= 5.59 (0.20) M_o, R_1=3.56 (0.07) R_o and T_eff1= 17000 (500) K for the primary and M_2=3.36 (0.14) M_o, R_2= 2.04 (0.05) R_o and T_eff2= 13140 (800) K for the secondary. Static absorbtion features apart from those coming from the close binary components are detected in the several spectral regions. If these absorbtion features are from a third star, as the light curve solutions support, its radial velocity is measured to be RV_3=70(8) km s^-1. The orbit of the binary system is proved to be eccentric (e=0.059) and thus the apsidal motion exists. The estimated linear advance in longitude of periastron corresponds to an apsidal motion of U=694+/-5 yr for the system. The average internal structure constant log k_2,obs...

  2. SHARPIN Regulates Uropod Detachment in Migrating Lymphocytes

    Directory of Open Access Journals (Sweden)

    Jeroen Pouwels

    2013-11-01

    Full Text Available SHARPIN-deficient mice display a multiorgan chronic inflammatory phenotype suggestive of altered leukocyte migration. We therefore studied the role of SHARPIN in lymphocyte adhesion, polarization, and migration. We found that SHARPIN localizes to the trailing edges (uropods of both mouse and human chemokine-activated lymphocytes migrating on intercellular adhesion molecule-1 (ICAM-1, which is one of the major endothelial ligands for migrating leukocytes. SHARPIN-deficient cells adhere better to ICAM-1 and show highly elongated tails when migrating. The increased tail lifetime in SHARPIN-deficient lymphocytes decreases the migration velocity. The adhesion, migration, and uropod defects in SHARPIN-deficient lymphocytes were rescued by reintroducing SHARPIN into the cells. Mechanistically, we show that SHARPIN interacts directly with lymphocyte-function-associated antigen-1 (LFA-1, a leukocyte counterreceptor for ICAM-1, and inhibits the expression of intermediate and high-affinity forms of LFA-1. Thus, SHARPIN controls lymphocyte migration by endogenously maintaining LFA-1 inactive to allow adjustable detachment of the uropods in polarized cells.

  3. Densiron® 68 as an intraocular tamponade for complex inferior retinal detachments

    Directory of Open Access Journals (Sweden)

    Hussain RN

    2011-05-01

    Full Text Available Rumana N Hussain, Somnath BanerjeeLeicester Royal Infirmary, Leicester, UKIntroduction: Densiron® 68 is a high-density liquid used to tamponade inferior retinal detachments. We present a case series of 12 patients treated with Densiron as an intraocular tamponade agent.Methods: A retrospective analysis of 12 eyes in 12 patients was carried out. The primary endpoint was anatomic reattachment of the retina following removal of Densiron oil.Results: All patients had inferior detachments; 33% had associated proliferative vitreoretinopathy (PVR. Densiron was utilized as a primary agent in five patients (42%; the remaining patients had prior unsuccessful surgery for retinal reattachment, including pars plana vitrectomy, cryotherapy, laser, encirclement, gas (C3F8 or C2F6, or silicone oil. Eleven patients (91% had successful reattachment of the retina at 3 months following removal of Densiron; one patient had extensive PVR, total retinal detachment, preretinal macula fibrosis, and chronic hypotony, and surgical intervention was unsuccessful. Six patients (50% had raised intraocular pressure (IOP, resolving in the majority of cases following Densiron removal; two patients had long-term raised IOP requiring topical or surgical therapy. Of the six phakic patients, 50% developed significant cataract in the operated eye. Of those with successful retinal reattachment, visual outcome was variable, with 36% patients gaining two to four lines on Snellen, 27% remaining objectively the same, and 36% losing one to two lines.Conclusion: The anatomic success rate is high (91% in patients requiring Densiron tamponade for inferior retinal detachments with or without evidence of PVR either as a primary or secondary intervention. A common complication is raised IOP; however, this most often resolves following removal of the oil.Keywords: intraocular tamponade, silicone oil, retinal detachment, retinal reattachments

  4. Contrasting Modes of Detachment Faulting at Slower-Spreading Mid-Ocean Ridges

    Science.gov (United States)

    MacLeod, C. J.; Deans, J. R.; Morris, A.; Evans, A.; Cheadle, M. J.; Ferrando, C.; Pluemper, O.; Viegas, G.; Ildefonse, B.; Dick, H. J.; Expedition 360 Scientists, I.

    2016-12-01

    Detachment-mode spreading is recognised as playing an essential role in accommodating plate separation at a substantial portion of the slower spreading mid-ocean ridge system. The basic geometry of oceanic detachments is now well established, from study of oceanic core complexes (OCCs) on the Mid-Atlantic Ridge (MAR) supported by numerical models. With a concave upwards geometry, faults form and slip at a steep angle sub-surface, flattening through a 'rolling hinge' as the footwall is exhumed and flexurally rotated, exposing flat or gently domed fault planes on the seafloor, often with marked spreading-direction-parallel corrugations. In well-studied examples (e.g. 15deg45N) strain localisation is achieved by formation and deformation of weak, relatively low-temperature phyllosilicates on the fault zone associated with penetration of water to deep levels on the detachment. Questions however remain about the style of deformation, controls and feedbacks between the locus of melt emplacement and locus of the deformation. In some cases gabbro bodies, emplaced within the detachment footwalls, are little affected by high-T crystal-plastic deformation (e.g. MAR at 15deg45N), in which case deformation is strongly localised in a narrow, low-T (greenschist facies) fault damage zone. In other cases, best typified by Atlantis Bank on the SW Indian Ridge, gabbros are instead characterised by extensive high-T crystal-plastic deformation. New drilling results from Atlantis Bank (IODP Expedition 360) emphasise the fundamentally different deformation mechanisms, likely geometry of detachment faulting, and rheology of newly-forming lithosphere in this magma-dominated, ultraslow-spreading system. We suggest these contrasting regions represent end-members of a broader spectrum of deformation styles at OCCs, controlled mostly by the locus and amount of magmatic heat input to the system and consequent rate of cooling of the active zone of deformation.

  5. Over-the-barrier electron detachment in the hydrogen negative ion

    CERN Document Server

    Milosevic, M Z

    2016-01-01

    The electron detachment from the hydrogen negative ion in strong fields is studied using the two-electron and different single-electron models within the quasistatic approximation. A special attention is payed to over-the-barrier regime where the Stark saddle is suppressed below the lowest energy level. It is demonstrated that the single-electron description of the lowest state of ion, that is a good approximation for weak fields, fails in this and partially in the tunneling regime. The exact lowest state energies and detachment rates for the ion at different strengths of the applied field are determined by solving the eigenvalue problem of the full two-electron Hamiltonian. An accurate formula for the rate, that is valid in both regimes, is determined by fitting the exact data to the expression estimated using single-electron descriptions.

  6. Rescue endoscopic bleeding control for nonvariceal upper gastrointestinal hemorrhage using clipping and detachable snaring.

    Science.gov (United States)

    Lee, J H; Kim, B K; Seol, D C; Byun, S J; Park, K H; Sung, I K; Park, H S; Shim, C S

    2013-06-01

    Nonvariceal upper gastrointestinal (UGI) bleeding recurs after appropriate endoscopic therapy in 10 % - 15 % of cases. The mortality rate can be as high as 25 % when bleeding recurs, but there is no consensus about the best modality for endoscopic re-treatment. The aim of this study was to evaluate clipping and detachable snaring (CDS) for rescue endoscopic control of nonvariceal UGI hemorrhage. We report a case series of seven patients from a Korean tertiary center who underwent endoscopic hemostasis using the combined method of detachable snares with hemoclips. The success rate of endoscopic hemostasis with CDS was 86 %: six of the seven patients who had experienced primary endoscopic treatment failure or recurrent bleeding after endoscopic hemostasis were treated successfully. In conclusion, rescue endoscopic bleeding control by means of CDS is an option for controlling nonvariceal UGI bleeding when no other method of endoscopic treatment for recurrent bleeding and primary hemostatic failure is possible.

  7. Section detachment in immunohistochemistry: causes, troubleshooting, and problem-solving.

    Science.gov (United States)

    Gambella, Alessandro; Porro, Lucia; Pigozzi, Simona; Fiocca, Roberto; Grillo, Federica; Mastracci, Luca

    2017-07-01

    Section detachment in immunohistochemistry (IHC) is a common phenomenon, increasing times and costs of diagnosis and research. However, it has poorly been investigated. The aim of this study was to identify the causes of section detachment, with the purpose of defining a quality assured laboratory procedure to minimize detachment frequency. We screened 3349 IHC sections from formalin-fixed paraffin-embedded tissue, identifying 177 cases with section detachment (5.3% of the sample). Detachment regarded mainly samples of surgical breast tissue and IHC procedures in which heat pretreatment was used. Focusing on pre-analytical factors, we investigated seven main critical issues: (1) section aging; (2) section thickness; (3) slide contamination; (4) slide aging; (5) slide brand; (6) "human" influence; and (7) sample size and fixation. Each of these issues was individually investigated to establish their influence on detachment. Targeted experiments were performed by varying section age, thickness, cleanliness, slide brand and age, and sample size and fixation. Finally, to investigate operator-dependent causes, sections were cut by different operators blinded to aim. The most important factors influencing section detachment were demonstrated to be: section thickness, slide aging, slide brand, "human" influence, and size and fixation of samples. The pre-analytical phase, including all the aforementioned issues, should be standardized within a quality assurance program. By adopting these recommendations, we obtained a 34% drop in section detachment. Although section detachment remains difficult to eradicate completely, many other influences can be addressed and corrected in any laboratory leading to an increase in efficiency and cost saving.

  8. Bacterial adhesion force quantification by fluidic force microscopy

    Science.gov (United States)

    Potthoff, Eva; Ossola, Dario; Zambelli, Tomaso; Vorholt, Julia A.

    2015-02-01

    Quantification of detachment forces between bacteria and substrates facilitates the understanding of the bacterial adhesion process that affects cell physiology and survival. Here, we present a method that allows for serial, single bacterial cell force spectroscopy by combining the force control of atomic force microscopy with microfluidics. Reversible bacterial cell immobilization under physiological conditions on the pyramidal tip of a microchanneled cantilever is achieved by underpressure. Using the fluidic force microscopy technology (FluidFM), we achieve immobilization forces greater than those of state-of-the-art cell-cantilever binding as demonstrated by the detachment of Escherichia coli from polydopamine with recorded forces between 4 and 8 nN for many cells. The contact time and setpoint dependence of the adhesion forces of E. coli and Streptococcus pyogenes, as well as the sequential detachment of bacteria out of a chain, are shown, revealing distinct force patterns in the detachment curves. This study demonstrates the potential of the FluidFM technology for quantitative bacterial adhesion measurements of cell-substrate and cell-cell interactions that are relevant in biofilms and infection biology.Quantification of detachment forces between bacteria and substrates facilitates the understanding of the bacterial adhesion process that affects cell physiology and survival. Here, we present a method that allows for serial, single bacterial cell force spectroscopy by combining the force control of atomic force microscopy with microfluidics. Reversible bacterial cell immobilization under physiological conditions on the pyramidal tip of a microchanneled cantilever is achieved by underpressure. Using the fluidic force microscopy technology (FluidFM), we achieve immobilization forces greater than those of state-of-the-art cell-cantilever binding as demonstrated by the detachment of Escherichia coli from polydopamine with recorded forces between 4 and 8 nN for many

  9. Shape Evolution of Detached Bridgman Crystals Grown in Microgravity

    Science.gov (United States)

    Volz, M. P.; Mazuruk, K.

    2015-01-01

    A theory describing the shape evolution of detached Bridgman crystals in microgravity has been developed. A starting crystal of initial radius r0 will evolve to one of the following states: Stable detached gap; Attachment to the crucible wall; Meniscus collapse. Only crystals where alpha plus omega is great than 180 degrees will achieve stable detached growth in microgravity. Results of the crystal shape evolution theory are consistent with predictions of the dynamic stability of crystallization (Tatarchenko, Shaped Crystal Growth, Kluwer, 1993). Tests of transient crystal evolution are planned for ICESAGE, a series of Ge and GeSi crystal growth experiments planned to be conducted on the International Space Station (ISS).

  10. Sensitivity of detachment extent to magnetic configuration and external parameters

    Science.gov (United States)

    Lipschultz, Bruce; Parra, Felix I.; Hutchinson, Ian H.

    2016-05-01

    Divertor detachment may be essential to reduce heat loads to magnetic fusion tokamak reactor divertor surfaces. Yet in experiments it is difficult to control the extent of the detached, low pressure, plasma region. At maximum extent the front edge of the detached region reaches the X-point and can lead to degradation of core plasma properties. We define the ‘detachment window’ in a given position control variable C (for example, the upstream plasma density) as the range in C within which the front location can be stably held at any position from the target to the X-point; increased detachment window corresponds to better control. We extend a 1D analytic model [1] to determine the detachment window for the following control variables: the upstream plasma density, the impurity concentration and the power entering the scrape-off layer (SOL). We find that variations in magnetic configuration can have strong effects; increasing the ratio of the total magnetic field at the X-point to that at the target, {{B}×}/{{B}t} , (total flux expansion, as in the super-x divertor configuration) strongly increases the detachment window for all control variables studied, thus strongly improving detachment front control and the capability of the divertor plasma to passively accommodate transients while still staying detached. Increasing flux tube length and thus volume in the divertor, through poloidal flux expansion (as in the snowflake or x-divertor configurations) or length of the divertor, also increases the detachment window, but less than the total flux expansion does. The sensitivity of the detachment front location, z h , to each control variable, C, defined as \\partial {{z}h}/\\partial C , depends on the magnetic configuration. The size of the radiating volume and the total divertor radiation increase \\propto {{≤ft({{B}×}/{{B}t}\\right)}2} and \\propto {{B}×}/{{B}t} , respectively, but not by increasing divertor poloidal flux expansion or field line length. We

  11. Evidences on Secular Dynamical Evolution of Detached Active Binary Orbits and Contact Binary Formation

    CERN Document Server

    Eker, Z; Bilir, S; Karatas, Y

    2006-01-01

    Evidence of secular dynamical evolution for detached active binary orbits are presented. First order decreasing rates of orbital angular momentum (OAM), systemic mass ($M=M_{1}+M_{2}$) and orbital period of detached active binaries have been determined as $\\dot J/J = 3.48 \\times 10^{-10}$yr$^{-1}$, $\\dot M/M = 1.30 \\times 10^{-10}$yr$^{-1}$ and $\\dot P/P = 3.96\\times 10^{-10}$yr$^{-1}$ from the kinematical ages of 62 field detached systems. The ratio of $d \\log J/ d \\log M = 2.68$ implies that either there are mechanisms which amplify AM loss $\\delta=2.68$ times with respect to isotropic AM loss of hypothetical isotropic winds or there exist external causes contributing AM loss in order to produce this mean rate of decrease for orbital periods. Various decreasing rates of OAM ($d \\log J / dt$) and systemic mass ($d \\log M/ dt$) determine various speeds of dynamical evolutions towards a contact configuration. According to average dynamical evolution with $\\delta = 2.68$, the fraction of 10, 22 and 39 per cent ...

  12. An investigation of the potential association between retinal detachment and oral fluoroquinolones: a self-controlled case series study.

    Science.gov (United States)

    Chui, Celine S L; Man, Kenneth K C; Cheng, Ching-Lan; Chan, Esther W; Lau, Wallis C Y; Cheng, Vincent C C; Wong, David S H; Yang Kao, Yea-Huei; Wong, Ian C K

    2014-09-01

    A study reported a significant association between oral fluoroquinolones and the development of retinal detachment among current users of oral fluoroquinolones (Etminan M, Forooghian F, Brophy JM et al. JAMA 2012; 307: 1414-9). However, other published studies have discordant results. This study aimed to investigate this association and to estimate the absolute risk of developing retinal detachment in patients exposed to oral fluoroquinolones. A self-controlled case series study was conducted with data retrieved from the Hong Kong Clinical Data Analysis and Reporting System database and the Taiwan National Health Insurance Research Database. Hong Kong and Taiwanese patients who had prescriptions for oral fluoroquinolones and a procedure for retinal detachment between 2001 and 2012 and between 2000 and 2010, respectively, were defined as cases and included in the analysis. A total of 9 events were found during the fluoroquinolone-exposed period and 1407 events were found during the non-exposed period. The adjusted incidence rate ratio in the combined model was 1.26 (0.65-2.47). The crude absolute risk of experiencing retinal detachment whilst on oral fluoroquinolones was ∼1.3 per 200 000 prescriptions. Our study does not support the association between the use of fluoroquinolones and the development of retinal detachment and our findings are strikingly similar to that of the study conducted in Denmark. Doubt is cast on the association between the use of fluoroquinolones and the development of retinal detachment. Therefore, the use of fluoroquinolones should not be precluded based on the current evidence on the risk of retinal detachment. The impact of different ethnicities on the response to fluoroquinolones should also be investigated. © The Author 2014. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  13. Retinal detachment after laser In Situ keratomileusis

    Directory of Open Access Journals (Sweden)

    Saba Al-Rashaed

    2011-01-01

    Full Text Available Purpose : To report characteristics and outcome of rhegmatogenous retinal detachment (RRD after laser in situ keratomileusis (LASIK for myopia. Materials and Methods : A retrospective chart review of patients who presented with RRD after myopic LASIK over a 10-year period. Results : Fourteen eyes were identified with RRD. Of these, two of 6112 LASIK procedures were from our center. The mean age of patients with RRD was 35.43 years. The mean interval of RRD after LASIK was 37.71 months (range, 4 months to 10 years. The macula was involved in eight eyes and spared in six eyes. Retinal breaks included a macular hole in two eyes, and giant tear in two eyes. Multiple breaks (>2 breaks occurred in 6 cases. Pars plana vitrectomy (PPV was performed in 3 (21.4% eyes, a scleral buckle (SB was performed in 4 (28.5% eyes and 7 (50% eyes underwent combined PPV and SB. Mean follow-up was 15.18 months (range, 1 month to 7 years. The retina was successfully attached in all cases. The final visual acuity was 20/40 or better in 7 (50% eyes, 20/40 to 20/60 in 4 (28.5% eyes, and 20/200 or less in 3 (21.4% eyes. Poor visual outcome was secondary to proliferative vitreoretinopathy, epiretinal membrane, macular scar and amblyopia. Conclusion : The prevalence of RRD after LASIK was low at our institute. Anatomical and visual outcomes were acceptable in eyes that were managed promptly. Although there is no cause-effect relationship between LASIK and RRD, a dilated fundus examination is highly recommended before and after LASIK for myopia.

  14. Advantages of diabetic tractional retinal detachment repair

    Science.gov (United States)

    Sternfeld, Amir; Axer-Siegel, Ruth; Stiebel-Kalish, Hadas; Weinberger, Dov; Ehrlich, Rita

    2015-01-01

    Purpose To evaluate the outcomes and complications of patients with diabetic tractional retinal detachment (TRD) treated with pars plana vitrectomy (PPV). Patients and methods We retrospectively studied a case series of 24 eyes of 21 patients at a single tertiary, university-affiliated medical center. A review was carried out on patients who underwent PPV for the management of TRD due to proliferative diabetic retinopathy from October 2011 to November 2013. Preoperative and final visual outcomes, intraoperative and postoperative complications, and medical background were evaluated. Results A 23 G instrumentation was used in 23 eyes (95.8%), and a 25 G instrumentation in one (4.2%). Mean postoperative follow-up time was 13.3 months (4–30 months). Visual acuity significantly improved from logarithm of the minimum angle of resolution (LogMAR) 1.48 to LogMAR 1.05 (P<0.05). Visual acuity improved by ≥3 lines in 75% of patients. Intraoperative complications included iatrogenic retinal breaks in seven eyes (22.9%) and vitreal hemorrhage in nine eyes (37.5%). In two eyes, one sclerotomy was enlarged to 20 G (8.3%). Postoperative complications included reoperation in five eyes (20.8%) due to persistent subretinal fluid (n=3), vitreous hemorrhage (n=1), and dislocated intraocular lens (n=1). Thirteen patients (54.2%) had postoperative vitreous hemorrhage that cleared spontaneously, five patients (20.8%) required antiglaucoma medications for increased intraocular pressure, seven patients (29.2%) developed an epiretinal membrane, and two patients (8.3%) developed a macular hole. Conclusion Patients with diabetic TRD can benefit from PPV surgery. Intraoperative and postoperative complications can be attributed to the complexity of this disease. PMID:26604667

  15. Bacterial Vaginosis

    Science.gov (United States)

    ... Form Controls Cancel Submit Search the CDC Bacterial Vaginosis (BV) Note: Javascript is disabled or is not ... STD on Facebook Sexually Transmitted Diseases (STDs) Bacterial Vaginosis – CDC Fact Sheet Language: English (US) Españ ...

  16. Serous retinal detachment after trabeculectomy in angle recession glaucoma

    Directory of Open Access Journals (Sweden)

    Roy, Avik Kumar

    2015-12-01

    Full Text Available An 18-year-old male with 360 degree angle recession after blunt trauma in his right eye developed uncontrolled intraocular pressure (IOP despite four antiglaucoma medications (AGM with advancing disc damage. He underwent trabeculectomy with intraoperative mitomycin-c (MMC application. There was an intraoperative vitreous prolapse which was managed accordingly. On post-surgery day 1, he had shallow choroidal detachment superiorly with non-recordable IOP. This was deteriorated 1 week postoperatively as choroidal detachment proceeded to serous retinal detachment. He was started with systemic steroid in addition to topical route. The serous effusions subsided within 2 weeks time. At the last follow up at 3 months, he was enjoying good visual acuity, deep anterior chamber, diffuse bleb, an IOP in low teens off any AGM and attached retina. This case highlights the rare occurrence of serous retinal detachment after surgical management of angle recession glaucoma.

  17. Inner detached frequency response curves: an experimental study

    Science.gov (United States)

    Gatti, Gianluca; Brennan, Michael J.

    2017-05-01

    Certain nonlinear vibrating systems have frequency response curves (FRCs), in which isolated detached curves exist inside the main continuous FRC. The behavior of these systems has hitherto been studied analytically and numerically, but to the authors' knowledge, there is no record of an inner detached FRC being detected experimentally. These curves may be hidden by numerical or experimental analysis, particularly when a system is subject to swept or stepped-sine excitation. Their existence may thus lead to unexpected dramatic changes in the amplitude of the system response. This paper presents an experimental study that involves the design, construction and testing of a specific system that has an isolated detached FRC inside the main continuous FRC. The experimental design of the test rig is supported by multibody dynamic simulations, and in the experimental tests the existence of a detached FRC was verified.

  18. Use of Perfluorocarbon Liquids in Complicated Retinal Detachments

    Institute of Scientific and Technical Information of China (English)

    B; C; Ang

    1992-01-01

    We now have an additional tool to help in managing complicated retinal detachments. We look forward to the day when we can leave the liquid without having to remove it because of the possibility of ocular complications.

  19. Tracing detached and attached care practices in nursing education

    DEFF Research Database (Denmark)

    Soffer, Ann Katrine B.

    2014-01-01

    The implementation of skills labs in Danish nursing education can, in itself, be viewed as a complexity. The students are expected to eventually carry out their work in a situated hospital practice, but they learn their professional skills in a different space altogether, detached and removed from...... of care are not explicated in the curriculum or textbooks; however, they surfaced once this crooked approach to studying care in a simulated practice was applied. The article starts from the assertion that detached engagements are not recognized within the field of nursing education as an equal component...... to attachments. Yet empirical cases from the skills lab and hospitals illustrate how students sometimes felt emotionally attached to plastic dummies and how experienced nurses sometimes practised a degree of detachment in relation to human patients. Detached engagements will therefore be presented as part...

  20. Method of detaching adherent cells for flow cytometry

    KAUST Repository

    Kaur, Mandeep

    2015-12-24

    In one aspect, a method for detaching adherent cells can include adding a cell lifting solution to the media including a sample of adherent cells and incubating the sample of adherent cells with the cell lifting solution. No scraping or pipetting is needed to facilitate cell detachment. The method do not require inactivation of cell lifting solution and no washing of detaching cells is required to remove cell lifting solution. Detached cells can be stained with dye in the presence of cell lifting solution and are further analyzed using flow cytometer. The method has been tested using 6 different cell lines, 4 different assays, two different plate formats (96 and 384 well plates) and two different flow cytometry instruments. The method is simple to perform, less time consuming, with no cell loss and makes high throughput flow cytometry on adherent cells a reality.

  1. Plasma detachment with molecular processes in divertor plasmas

    Energy Technology Data Exchange (ETDEWEB)

    Ohno, N.; Ezumi, N.; Nishijima, D.; Takamura, S. [Dept. of Energy Engineering and Science, Graduate School of Engineering, Nagoya Univ., Nagoya, Aichi (Japan); Krasheninnikov, S.I.; Pigarov, A.Yu. [MIT Plasma Science and Fusion Center, Cambridge, MA (United States)

    2000-01-01

    Molecular processes in detached recombining plasmas are briefly reviewed. Several reactions with vibrationally excited hydrogen molecule related to recombination processes are described. Experimental evidence of molecular activated recombination observed in a linear divertor plasma simulator is also shown. (author)

  2. The study of organic removal efficiency and halophilic bacterial mixed liquor characteristics in a membrane bioreactor treating hypersaline produced water at varying organic loading rates.

    Science.gov (United States)

    Sharghi, Elham Abdollahzadeh; Bonakdarpour, Babak

    2013-12-01

    In this study the organic pollutant removal performance and the mixed liquor characteristics of a membrane bioreactor (MBR), employing a halophilic bacterial consortium, for the treatment of hypersaline synthetic produced water - at varying organic loading rates (OLR) from 0.3 to 2.6 kg CODm(-3)d(-1) - were considered. The oil and grease (O&G) and COD removal efficiency were 95-99% and 83-93%, respectively with only transient O&G (mainly polycyclic aromatic hydrocarbons) and soluble microbial products accumulation being observed. With increasing OLR, in the range 0.9-2.6 kg COD m(-3)d(-1), as a result of change in both extracellular polymeric substances (EPS) and zeta potential, bioflocculating ability improved but the compressibility of the flocs decreased resulting in the occurrence of EPS bulking at the highest OLR studied. The latter resulted in a change in the rheology of the mixed liquor from Newtonian to non-Newtonian and the occurrence of significant membrane fouling. Copyright © 2013 Elsevier Ltd. All rights reserved.

  3. Impact of restricted amoxicillin/clavulanic acid use on Escherichia coli resistance--antibiotic DU90% profiles with bacterial resistance rates: a visual presentation.

    Science.gov (United States)

    Mimica Matanovic, Suzana; Bergman, Ulf; Vukovic, Dubravka; Wettermark, Björn; Vlahovic-Palcevski, Vera

    2010-10-01

    High use of amoxicillin/clavulanic acid (AMC) at the University Hospital Osijek (Croatia) contributed to high rates of resistance in Enterobacteriaceae, in particular Escherichia coli (50%). Thus, in order to decrease bacterial resistance, AMC use was restricted. We present results of the restriction on resistance amongst antibiotics accounting for 90% of antibiotic use [drug utilisation 90% (DU90%)]. Data were analysed on antibiotic use and microbiological susceptibility of E. coli during two 9-month periods, before and after the restriction of AMC use. Drug use was presented as numbers of defined daily doses (DDDs) and DDDs/100 bed-days. Resistance of E. coli to antibiotics was presented as percentages of isolated strains in the DU90% segment. Use of AMC was 16 DDDs/100 bed-days or 30% of all antibiotics before the intervention. Use of AMC fell to 2 DDDs/100 bed-days or 4% after the intervention, and resistance of E. coli fell from 37% to 11%. In conclusion, restricted use of AMC resulted in a significant decrease of E. coli resistance. DU90% resistance profiles are simple and useful tools in highlighting problems in antibiotic use and resistance but may also be useful in long-term follow-up of antibiotic policy.

  4. Ventilator-associated pneumonia: the influence of bacterial resistance, prescription errors, and de-escalation of antimicrobial therapy on mortality rates

    Directory of Open Access Journals (Sweden)

    Ana Carolina Souza-Oliveira

    Full Text Available Abstract Ventilator-associated pneumonia is the most prevalent nosocomial infection in intensive care units and is associated with high mortality rates (14–70%. Aim This study evaluated factors influencing mortality of patients with Ventilator-associated pneumonia (VAP, including bacterial resistance, prescription errors, and de-escalation of antibiotic therapy. Methods This retrospective study included 120 cases of Ventilator-associated pneumonia admitted to the adult adult intensive care unit of the Federal University of Uberlândia. The chi-square test was used to compare qualitative variables. Student's t-test was used for quantitative variables and multiple logistic regression analysis to identify independent predictors of mortality. Findings De-escalation of antibiotic therapy and resistant bacteria did not influence mortality. Mortality was 4 times and 3 times higher, respectively, in patients who received an inappropriate antibiotic loading dose and in patients whose antibiotic dose was not adjusted for renal function. Multiple logistic regression analysis revealed the incorrect adjustment for renal function was the only independent factor associated with increased mortality. Conclusion Prescription errors influenced mortality of patients with Ventilator-associated pneumonia, underscoring the challenge of proper Ventilator-associated pneumonia treatment, which requires continuous reevaluation to ensure that clinical response to therapy meets expectations.

  5. Forces involved in bacterial adhesion to hydrophilic and hydrophobic surfaces.

    Science.gov (United States)

    Boks, Niels P; Norde, Willem; van der Mei, Henny C; Busscher, Henk J

    2008-10-01

    Using a parallel-plate flow chamber, the hydrodynamic shear forces to prevent bacterial adhesion (F(prev)) and to detach adhering bacteria (F(det)) were evaluated for hydrophilic glass, hydrophobic, dimethyldichlorosilane (DDS)-coated glass and six different bacterial strains, in order to test the following three hypotheses. 1. A strong hydrodynamic shear force to prevent adhesion relates to a strong hydrodynamic shear force to detach an adhering organism. 2. A weak hydrodynamic shear force to detach adhering bacteria implies that more bacteria will be stimulated to detach by passing an air-liquid interface (an air bubble) through the flow chamber. 3. DLVO (Derjaguin, Landau, Verwey, Overbeek) interactions determine the characteristic hydrodynamic shear forces to prevent adhesion and to detach adhering micro-organisms as well as the detachment induced by a passing air-liquid interface. F(prev) varied from 0.03 to 0.70 pN, while F(det) varied from 0.31 to over 19.64 pN, suggesting that after initial contact, strengthening of the bond occurs. Generally, it was more difficult to detach bacteria from DDS-coated glass than from hydrophilic glass, which was confirmed by air bubble detachment studies. Calculated attractive forces based on the DLVO theory (F(DLVO)) towards the secondary interaction minimum were higher on glass than on DDS-coated glass. In general, all three hypotheses had to be rejected, showing that it is important to distinguish between forces acting parallel (hydrodynamic shear) and perpendicular (DLVO, air-liquid interface passages) to the substratum surface.

  6. Bacterial Vaginosis

    Science.gov (United States)

    ... Issues > Conditions > Sexually Transmitted > Bacterial Vaginosis Health Issues Listen Español Text Size Email Print Share Bacterial Vaginosis Page Content Bacterial vaginosis (BV) is the most common vaginal infection in sexually active teenaged girls . It appears to be caused by ...

  7. Detached eclipsing binaries with very unequal members - HR 7464

    Science.gov (United States)

    Giuricin, G.; Mardirossian, F.; Mezzetti, M.

    1984-06-01

    Waelkens and Rufener's (1983) photoelectric lightcurve (as yet unexplored) of the newly discovered eclipsing binary HR 7464 has been analyzed. The photometric solution presented here reveals that this binary is an A5m + G main sequence detached system, which is particularly remarkable for the great dissimilarity between its members. The frequency of detached eclipsing pairs with very unequal members (i.e., with low mass ratio) is then discussed; some bimodality in the innate mass ratio distribution of close binaries is inferred.

  8. Scleral buckling for retinal detachment in patients with retinoblastoma

    Energy Technology Data Exchange (ETDEWEB)

    Buzney, S.M.; Pruett, R.C.; Regan, C.D.; Walton, D.S.; Smith, T.R.

    1984-10-15

    Three children (two girls and one boy) with bilateral retinoblastoma each developed a presumed rhegmatogenous retinal detachment in one eye. All three eyes had previously received radiation and cryotherapy. In each case the retinal detachment responded promptly to conventional surgical methods via scleral buckling in the area of treated retinoblastoma and presumed retinal break. All three eyes have retained useful vision for follow-up periods of 3.5 to 12 years.

  9. HAMLET binding to α-actinin facilitates tumor cell detachment.

    Science.gov (United States)

    Trulsson, Maria; Yu, Hao; Gisselsson, Lennart; Chao, Yinxia; Urbano, Alexander; Aits, Sonja; Mossberg, Ann-Kristin; Svanborg, Catharina

    2011-03-08

    Cell adhesion is tightly regulated by specific molecular interactions and detachment from the extracellular matrix modifies proliferation and survival. HAMLET (Human Alpha-lactalbumin Made LEthal to Tumor cells) is a protein-lipid complex with tumoricidal activity that also triggers tumor cell detachment in vitro and in vivo, suggesting that molecular interactions defining detachment are perturbed in cancer cells. To identify such interactions, cell membrane extracts were used in Far-western blots and HAMLET was shown to bind α-actinins; major F-actin cross-linking proteins and focal adhesion constituents. Synthetic peptide mapping revealed that HAMLET binds to the N-terminal actin-binding domain as well as the integrin-binding domain of α-actinin-4. By co-immunoprecipitation of extracts from HAMLET-treated cancer cells, an interaction with α-actinin-1 and -4 was observed. Inhibition of α-actinin-1 and α-actinin-4 expression by siRNA transfection increased detachment, while α-actinin-4-GFP over-expression significantly delayed rounding up and detachment of tumor cells in response to HAMLET. In response to HAMLET, adherent tumor cells rounded up and detached, suggesting a loss of the actin cytoskeletal organization. These changes were accompanied by a reduction in β1 integrin staining and a decrease in FAK and ERK1/2 phosphorylation, consistent with a disruption of integrin-dependent cell adhesion signaling. Detachment per se did not increase cell death during the 22 hour experimental period, regardless of α-actinin-4 and α-actinin-1 expression levels but adherent cells with low α-actinin levels showed increased death in response to HAMLET. The results suggest that the interaction between HAMLET and α-actinins promotes tumor cell detachment. As α-actinins also associate with signaling molecules, cytoplasmic domains of transmembrane receptors and ion channels, additional α-actinin-dependent mechanisms are discussed.

  10. Simulating Biofilm Deformation and Detachment with the Immersed Boundary Method

    Science.gov (United States)

    Sudarsan, Rangarajan; Ghosh, Sudeshna; Stockie, John M.; Eberl, Hermann J.

    2016-03-01

    We apply the immersed boundary (or IB) method to simulate deformation and detachment of a periodic array of wall-bounded biofilm colonies in response to a linear shear flow. The biofilm material is represented as a network of Hookean springs that are placed along the edges of a triangulation of the biofilm region. The interfacial shear stress, lift and drag forces acting on the biofilm colony are computed by using fluid stress jump method developed by Williams, Fauci and Gaver [Disc. Contin. Dyn. Sys. B 11(2):519-540, 2009], with a modified version of their exclusion filter. Our detachment criterion is based on the novel concept of an averaged equivalent continuum stress tensor defined at each IB point in the biofilm which is then used to determine a corresponding von Mises yield stress; wherever this yield stress exceeds a given critical threshold the connections to that node are severed, thereby signalling the onset of a detachment event. In order to capture the deformation and detachment behaviour of a biofilm colony at different stages of growth, we consider a family of four biofilm shapes with varying aspect ratio. Our numerical simulations focus on the behaviour of weak biofilms (with relatively low yield stress threshold) and investigate features of the fluid-structure interaction such as locations of maximum shear and increased drag. The most important conclusion of this work is that the commonly employed detachment strategy in biofilm models based only on interfacial shear stress can lead to incorrect or inaccurate results when applied to the study of shear induced detachment of weak biofilms. Our detachment strategy based on equivalent continuum stresses provides a unified and consistent IB framework that handles both sloughing and erosion modes of biofilm detachment, and is consistent with strategies employed in many other continuum based biofilm models.

  11. Retinal Detachment due to CrossFit Training Injury

    OpenAIRE

    Stephanie A. Joondeph; Brian C. Joondeph

    2013-01-01

    The purpose of this paper is to describe a traumatic retinal detachment occurring as a result of CrossFit training using an elastic exercise band. The patient sustained an ocular injury from an elastic band during CrossFit training, resulting in a giant retinal dialysis and retinal detachment, which were successfully repaired. Trainers and athletes need to be aware of the potential for ocular injury from elastic exercise bands and take appropriate precautions.

  12. Detachment and flow cytometric quantification of seagrass-associated bacteria.

    Science.gov (United States)

    Trevathan-Tackett, Stacey; Macreadie, Peter; Ralph, Peter; Seymour, Justin

    2014-07-01

    A new protocol was developed to detach bacteria from seagrass tissue and subsequently enumerate cells using flow cytometry (FCM). A method involving addition of the surfactant Tween 80 and vortexing resulted in maximum detachment efficiency of seagrass attached bacteria, providing a robust protocol for precisely enumerating seagrass-associated bacteria with FCM. Using this approach we detected cell concentrations between 2.0×10(5) and 8.0×10(6)cells mg(-1) DW tissue.

  13. Retinal Detachment due to CrossFit Training Injury

    Directory of Open Access Journals (Sweden)

    Stephanie A. Joondeph

    2013-01-01

    Full Text Available The purpose of this paper is to describe a traumatic retinal detachment occurring as a result of CrossFit training using an elastic exercise band. The patient sustained an ocular injury from an elastic band during CrossFit training, resulting in a giant retinal dialysis and retinal detachment, which were successfully repaired. Trainers and athletes need to be aware of the potential for ocular injury from elastic exercise bands and take appropriate precautions.

  14. Retinal Detachment due to CrossFit Training Injury

    OpenAIRE

    Stephanie A. Joondeph; Joondeph, Brian C.

    2013-01-01

    The purpose of this paper is to describe a traumatic retinal detachment occurring as a result of CrossFit training using an elastic exercise band. The patient sustained an ocular injury from an elastic band during CrossFit training, resulting in a giant retinal dialysis and retinal detachment, which were successfully repaired. Trainers and athletes need to be aware of the potential for ocular injury from elastic exercise bands and take appropriate precautions.

  15. Retinal Detachment due to CrossFit Training Injury.

    Science.gov (United States)

    Joondeph, Stephanie A; Joondeph, Brian C

    2013-01-01

    The purpose of this paper is to describe a traumatic retinal detachment occurring as a result of CrossFit training using an elastic exercise band. The patient sustained an ocular injury from an elastic band during CrossFit training, resulting in a giant retinal dialysis and retinal detachment, which were successfully repaired. Trainers and athletes need to be aware of the potential for ocular injury from elastic exercise bands and take appropriate precautions.

  16. Duration of rhegmatogenous retinal detachment predicts recovery of retinal sensitivity

    Directory of Open Access Journals (Sweden)

    Rose Rose

    2016-02-01

    Full Text Available The decision to treat a disease is often based on the presence or absence of symptoms, one prototype case being rhegmatogenous retinal detachment. Detachment of the neural retina from the pigment epithelium is a major cause of anatomical and functional dysfunction of the retina, where retinal recovery is inversely related to duration of detachment. The purpose of retinal reattachment is to effect recovery of the photoreceptors and pigment epithelium from degeneration. The aim of this study was to determine the critical duration of rhegmatogenous retinal detachment resulting in optimal retinal recovery after reattachment. A prospective study was conducted at a private hospital in Yogyakarta. Thirty five eyes were involved in this study. Three months after reattachment, central retinal recovery was measured by means of a Goldmann manual kinetic perimeter. The results showed that retinal recovery developed three months after surgery if the onset of rhegmatogenous retinal detachment was less than 28 days before surgery. The results were not significant if the onset of rhegmatogenous retinal detachment was more than 35 days. Although the Goldmann manual kinetic perimeter can efficiently detect central retinal sensitivity, it should be supported by more sensitive tools to evaluate the anatomy and function of the retina.

  17. OUTCOMES OF RETINAL DETACHMENT REPAIR AFTER POSTERIOR OPEN GLOBE INJURY.

    Science.gov (United States)

    Reed, David C; Juhn, Alexander T; Rayess, Nadim; Hsu, Jason; Chiang, Allen

    2016-04-01

    To report outcomes of retinal detachment (RD) repair following posterior open globe injury. This retrospective, consecutive case series examined patients who underwent RD repair following Zone II and/or III open globe injury repair between January 1, 2007 and October 31, 2013. Patients with globe injury repair were excluded. Of 30 patients who met inclusion criteria, reattachment of the retina was achieved in 25 (83%) during the first vitreoretinal surgical procedure and 5 (17%) were deemed inoperable intraoperatively. Ten patients (30%) developed recurrent RD, and 8 underwent additional surgery. At last follow-up, reattachment was observed in 4 of these 8. The overall rate of final reattachment was 63% (19 patients). The mean number of surgeries for RD was 1.5 (range, 1-3). Fifteen patients (50%) achieved final visual acuity of counting fingers or better. Mean follow-up from the last vitreoretinal surgery was 23 months (range, 3-52). Although RD following posterior open globe injury confers a grave prognosis, successful anatomic reattachment of the retina was achieved in the majority of patients in this series, with half achieving ambulatory vision.

  18. First period investigation of detached binary star AM Tau

    Science.gov (United States)

    Wang, Z.-H.; Zhu, L.-Y.

    2016-11-01

    AM Tau is a detached eclipsing binary with the secondary component more evolved than the primary one. The period changes of this neglected eclipsing binary are analyzed based on four times of primary minimum obtained between 2007 and 2016 together with those collected from the literature. It is detected that the general trend of the O-C curve shows a downward parabolic variation, which reveals a continuous period decrease at a rate of dP / dt = - 9.0 ×10-7(± 0.2) d yr-1 , meanwhile, the system undergoes two cyclic oscillations with periods of 24.1 and 8.8 years. The continuous decrease in the orbital period may be caused by angular momentum loss (AML) via an enhanced stellar wind of the evolved secondary star. The two cyclic variations in the O-C diagram are interpreted by the light travel-time effect via the presence of two additional stellar companions with masses estimated as M3sin i3 ∼ 1.36 M⊙ and M4sin i4 ∼ 0.33 M⊙. Their orbital separations are about 14 and 8 AU, respectively, and the orbital periods are almost in 3 : 1 resonance orbits.

  19. Bacterial Sialidase

    Science.gov (United States)

    2004-01-01

    Data shows that elevated sialidase in bacterial vaginosis patients correlates to premature births in women. Bacterial sialidase also plays a significant role in the unusual colonization of Pseudomonas aeruginosa in cystic fibrosis patients. Crystals of Salmonella sialidase have been reproduced and are used for studying the inhibitor-enzyme complexes. These inhibitors may also be used to inhibit a trans-sialidase of Trypanosome cruzi, a very similar enzyme to bacterial sialidase, therefore preventing T. cruzi infection, the causitive agent of Chagas' disease. The Center for Macromolecular Crystallography suggests that inhibitors of bacterial sialidases can be used as prophylactic drugs to prevent bacterial infections in these critical cases.

  20. Evolving techniques in the treatment of macular detachment caused by optic nerve pits.

    Science.gov (United States)

    Cox, M S; Witherspoon, C D; Morris, R E; Flynn, H W

    1988-07-01

    Fourteen patients with macular detachments caused by optic nerve pits were treated for progressive visual loss, cystoid macular changes, or atrophy of the macular retinal pigment epithelium. Photocoagulation of the temporal disc border alone was unsuccessful in two cases but promptly reattached the macula of two patients immobilized after laser surgery. Vitrectomy and gas tamponade improved vision and flattened the macula of three patients over various periods. The detachment recurred in one patient. Prompt and sustained macular reattachment with improved vision was noted after photocoagulation, vitrectomy, and gas tamponade in eight patients, although four required second operations. The prompt reattachment and visual recovery noted in these eight patients surpasses the reported 25% rate of spontaneous resolution.

  1. Fluid flow in extensional detachments determined from stable isotope analyses: Application to Kettle dome detachment, Washington, USA

    Science.gov (United States)

    Quilichini, A.; Teyssier, C.; Mulch, A.; Nachlas, W.

    2009-04-01

    In detachment systems that border metamorphic core complexes fluids convect from the surface to the detachment along faults and fractures in the brittle crust that serve as zones of recharge and discharge. This buoyancy-driven fluid flow is controlled by a high heat flow at the base of the system, beneath the detachment, where heat is advected by crustal thinning and magma intrusions. This hydrothermal convective flow is focused in the detachment for the duration of activity of the detachment and at relatively high temperature (300-500°C), resulting in very significant fluid-rock interaction and isotopic exchange. Studies of detachments in the North American Cordilleran core complexes suggest that meteoric fluids permeate detachment zones, as recorded by the deuterium composition of hydrous phases such as white mica, biotite, and amphibole. Quantifying fluid flux in detachments is a challenge because permeability of ductilely deforming rocks is poorly understood. The approach we are using focuses on oxygen and hydrogen isotopes in quartzite (+ minor mica) sections of detachments, complemented by high-precision chemical analyses of mica to understand their growth history and recrystallization process. The initial fluid isotopic composition is approximated using the deuterium composition of mica at a particular temperature that is given by oxygen isotopes in quartz-mica pairs. The more fluid interact with the quartzite, the larger the expected shift in oxygen isotope value. The Eocene Kettle Dome detachment in the North American Cordillera provides a continuous section of ~200 m thick quartzite mylonite where this methodology is applied. High-resolution sampling (up to 5 m) complements the initial sampling that was performed every 10 m in this section (Mulch et al., 2006, Tectonics, TC4001). Based on mica deuterium values, the fluid that participated in mica crystallization was meteoric in origin (~110 per mil). Interaction of this fluid with the quartz mylonite

  2. The potential and need for energy saving in standard family detached and semi-detached wooden houses in arctic Greenland

    DEFF Research Database (Denmark)

    Bjarløv, Søren Peter; Vladyková, Petra

    2011-01-01

    The paper gives an account of the potential and need for energy saving in standard family detached and semi-detached wooden houses in Greenland. It is based on studies of house construction compared with Building Regulation requirements and the spread of buildings over time. In the climatic...... to approximately half of the housing stock without changing the architectural expression or having to relocate the occupants during the renovation....

  3. Anatomical and functional outcomes of scleral buckling versus primary vitrectomy in pseudophakic retinal detachment.

    Science.gov (United States)

    Cankurtaran, Veysel; Citirik, Mehmet; Simsek, Mert; Tekin, Kemal; Teke, Mehmet Yasin

    2017-02-21

    Retinal detachment is the separation of the sensory retina from the retinal pigment epithelium by subretinal fluid. There are several types of retinal re-attachment surgery, including scleral buckling (SB), pneumatic retinopexy, and vitrectomy (with or without SB). The objective of this study was to compare anatomical and visual outcomes between patients with pseudophakic rhegmatogenous retinal detachment (RRD) who underwent pars plana vitrectomy (PPV) with silicone oil (SO) or perfluoropropane (C3F8) gas tamponade and pseudophakic RRD patients who underwent SB surgery. We evaluated retrospectively 101 pseudophakic RRD patients from a single center. The patients were classified into three groups according to the surgical procedure performed: PPV + Silicone - patients who underwent PPV with SO tamponade; PPV + Gas - patients who underwent PPV with perfluoropropane gas tamponade; and SB group - patients who underwent SB surgery. The groups were compared with regard to primary and final anatomical and visual outcomes. The number of patients in PPV + Silicone, PPV + Gas, and SB group was 39 (38.6%), 32 (31.7%), and 30 (29.7%), respectively. The mean follow-up period in PPV + Silicone, PPV + Gas, and SB group was 33.95 ± 23.58, 32.62 ± 10.95, and 33.76 ± 16.62 months, respectively. No significant difference was observed between the groups neither with regard to primary and final anatomical and visual success rates nor in relation to the recurrence rate of retinal detachment. According to our anatomical and visual outcome results, either of the three methods (i.e., PPV + Silicone, PPV + Gas, or SB) can be used in the treatment of pseudophakic retinal detachment.

  4. Anatomical and functional outcomes of scleral buckling versus primary vitrectomy in pseudophakic retinal detachment

    Science.gov (United States)

    Cankurtaran, Veysel; Citirik, Mehmet; Simsek, Mert; Tekin, Kemal; Teke, Mehmet Yasin

    2017-01-01

    Retinal detachment is the separation of the sensory retina from the retinal pigment epithelium by subretinal fluid. There are several types of retinal re-attachment surgery, including scleral buckling (SB), pneumatic retinopexy, and vitrectomy (with or without SB). The objective of this study was to compare anatomical and visual outcomes between patients with pseudophakic rhegmatogenous retinal detachment (RRD) who underwent pars plana vitrectomy (PPV) with silicone oil (SO) or perfluoropropane (C3F8) gas tamponade and pseudophakic RRD patients who underwent SB surgery. We evaluated retrospectively 101 pseudophakic RRD patients from a single center. The patients were classified into three groups according to the surgical procedure performed: PPV + Silicone - patients who underwent PPV with SO tamponade; PPV + Gas - patients who underwent PPV with perfluoropropane gas tamponade; and SB group - patients who underwent SB surgery. The groups were compared with regard to primary and final anatomical and visual outcomes. The number of patients in PPV + Silicone, PPV + Gas, and SB group was 39 (38.6%), 32 (31.7%), and 30 (29.7%), respectively. The mean follow-up period in PPV + Silicone, PPV + Gas, and SB group was 33.95 ± 23.58, 32.62 ± 10.95, and 33.76 ± 16.62 months, respectively. No significant difference was observed between the groups neither with regard to primary and final anatomical and visual success rates nor in relation to the recurrence rate of retinal detachment. According to our anatomical and visual outcome results, either of the three methods (i.e. PPV + Silicone, PPV + Gas, or SB) can be used in the treatment of pseudophakic retinal detachment. PMID:28135566

  5. Improving the Outcome of Rhegmatogenous Retinal Detachment Repair by Adding Pieces to the Puzzle : Historical review and recent contributions

    NARCIS (Netherlands)

    M.A.H. Veckeneer (Marc)

    2015-01-01

    markdownabstractAbstract Thanks to the consecutive introduction of penetrating thermocautery, buckling surgery and pars plana vitrectomy, the anatomical success rate of rhegmatogenous retinal detachment (RRD) repair improved dramatically, from 1/1000 about a hundred years ago, to about 90% by the

  6. Improving the Outcome of Rhegmatogenous Retinal Detachment Repair by Adding Pieces to the Puzzle : Historical review and recent contributions

    NARCIS (Netherlands)

    M.A.H. Veckeneer (Marc)

    2015-01-01

    markdownabstractAbstract Thanks to the consecutive introduction of penetrating thermocautery, buckling surgery and pars plana vitrectomy, the anatomical success rate of rhegmatogenous retinal detachment (RRD) repair improved dramatically, from 1/1000 about a hundred years ago, to about 90% by

  7. Human lactobacilli as supplementation of clindamycin to patients with bacterial vaginosis reduce the recurrence rate; a 6-month, double-blind, randomized, placebo-controlled study

    Directory of Open Access Journals (Sweden)

    Ryttig Kjeld R

    2008-01-01

    Full Text Available Abstract Background The primary objective of this study was to investigate if supplementary lactobacilli treatment could improve the initial cure rate after vaginal clindamycin therapy, and secondly, if lactobacilli as repeated adjunct treatment during 3 menstrual cycles could lengthen the time to relapse after initial cure. Methods Women (n = 100 with bacterial vaginosis diagnosed by Amsel criteria were after informed consent offered vaginal clindamycin therapy followed by vaginal gelatine capsules containing either 109 freeze-dried lactobacilli or identical placebo capsules for 10 days during 3 menstrual cycles in a double-blind, randomized, placebo-controlled trial. Results The initial intent to treat (ITT analysis for the one-month cure rate was 64% in the lactobacilli group and 78% in the placebo group (p > 0.05. However, any patient with missing or unclassified smears at the initial visit who continued the study and whose next smear indicated a cure was included in the cured group; the study also excluded two of the patients in the lactobacilli group who reported that they did not take any vaginal capsules. With consideration to these population changes, the initial cure rate would be 77% in the lactobacilli group. The 76 cured women were followed for 6 menstrual cycles or until relapse within that time span. At the end of the study, 64.9% (24/37 of the lactobacilli treated women were still BV-free compared to 46.2% (18/39 of the placebo treated women. Comparison of the two groups regarding "Time from cure to relapse" was statistically significant (p = 0.027 in favour of the lactobacilli treatment. Adjuvant therapy with lactobacilli contributed significantly to avoidance of relapse with a proportional Hazard Risk ratio (HR of 0.73 (0.54–0.98 (p Conclusion The study shows that supplementary treatment combining two different strains of probiotic lactobacilli does not improve the efficacy of BV therapy during the first month of treatment

  8. INITIAL COMPARISON OF INTRACRANIAL ANEURYSM EMBOLIZATION WITH MECHANICAL DETACHABLE SPIRALS AND WITH GUGLIELMI DETACHABLE COILS

    Institute of Scientific and Technical Information of China (English)

    王大明; 凌锋; 王安顺; 蔡艺龄; 李萌

    2003-01-01

    Objective.To compare the embolization effects of intracranial aneurysms with mechanical detachable spirals(MDS)and with Guglielmi detachable coils(GDC). Methods. One hundred and twenty cases with 125 intracranial aneurysms were embolized in Beijing Hospital from March 1995 to July 1999. Sixty-six aneurysms in 64 cases were embolized with MDS,51 in 48 with GDC,and 8 in 8 with both MDS and GDC.Clinical data including sex,age,subarachnoid hemorrhage(SAH),Hunt & Hess grading,diameter and neck width of aneurysms,number and length of coils used per aneurysm,occlusive ratio,and complications were compared between MDS and GDC groups. Results. MDS and GDC groups were comparable(t-test or χ2 -test,all P value >0.10)in terms of age,sex,diameter of aneurysms[(8.46±3.42)mm vs.(7.38±3.45)mm],neck width[(3.49±1.50)mm vs.(3.26±1.52)mm],coils number[(4.65±3.01)vs.(4.24±2.65)]and their length[(460.2±398.5)mm vs.(422.9±387.1)mm]used per aneurysm,occlusive ratio in aneurysms embolized≥80%[(95.00%±6.32%)vs.(94.19%±7.63%)],mortality and permanent complications(7.8% vs. 4.2%). Conclusions. MDS and GDC are all materials for embolization of intracranial aneurysms. MDS is less expensive,but more difficult to control and of propensity to complications while GDC is more compliant,easier to be used,safer,and have many alternative types for use as well as more extensive indications.

  9. ABDOMINAL CLOSURE WITH ANTI BACTERIAL COATED SUTURE MATERIALS AND ITS RELATION TO THE INCIDENCE OF POST OPERATIVE SUPERFICIAL SURGICAL SITE INFECTION RATES

    Directory of Open Access Journals (Sweden)

    Josephine Pudumai Selvi

    2017-07-01

    Full Text Available BACKGROUND Surgical site infection (SSI is an immense burden on healthcare resources even in the modern era of immaculate sterilization approaches and highly effective antibiotics. An estimated 234 million various surgical procedures, involving skin incisions requiring various types of wound closure techniques, are performed in the world, with the majority resulting in a wound healing by primary intention. Triclosan (5-chloro-2-(2, 4-dichlorophenoxy phenol is a broad-spectrum bactericidal agent that has been used for more than 40 years in various products, such as toothpaste and soaps. Higher concentrations of Triclosan work as a bactericide by attacking different structures in the bacterial cytoplasm and cell membrane. Use of Triclosan-coated sutures should theoretically result in the reduction of SSI. The aim of the study is to assess the abdominal closure with antibacterial coated suture materials and its relation to the incidence of post-operative superficial surgical site infection rates. MATERIALS AND METHODS The data will be collected from hospital records of surgery performed, post-operative daily progress notes and outpatient folders and telephonic conversations with patients after discharge. All patients undergoing laparotomy procedure for any cause. 100 patients divided as 50 in each group. RESULTS The positive outcome of infection (21.5% in patients using ordinary sutures was significantly differed with the positive outcome of infection (11.4% of Triclosan coated sutures. CONCLUSION In conclusion since there was a definite advantage inferred to the patients by using Triclosan coated polyglactin 910, it is the opinion of the researcher that Triclosan coated sutures has a role to play in reducing SSI in clean wounds and its use should be confined to areas where its application has proven benefits. However more studies should be done to clearly define its role and indications in surgery.

  10. Mesospheric electron detachment and LORE recovery times

    Science.gov (United States)

    Gordillo-Vazquez, Francisco J.; Haldoupis, Christos; Luque, Alejandro

    2015-04-01

    We present new results concerning the recovery times (> 10 minutes) of LOng Recovery Early VLF events (LORE) in the upper mesosphere connected to electromagnetic pulses (EMP) of large (> 250 kA km) charge moment change (CMC) ± CG (cloud to ground) lightning capable of producing elves or elve-sprite pairs (in the case of +CG parent lightning) [1], [2]. We have modeled two possible scenarios considering first the relaxation of slightly perturbed ambient electron densities (ne0 + Δne) without an impulsive ionization source and another scenario where the ambient electron density is considerably enhanced due to an impulsive ionization source (the lightning EMP). The full non-equilibrium kinetic and 2D EMP modelling of the perturbed mesosphere in the 76 km - 92 km range during LORE occurring conditions indicates that the electron density relaxation time (defined as the time the perturbed electron density, Δne, takes to decay a factor 1/e of the way to the ambient electron density (ne0)) is critically controlled at each altitude by the relative importance of associative detachment (of O- by, respectively, O and CO and of O2- by O) with respect to electron loss mechanisms (mainly 3-body, 2-body attachment and electron-ion recombination at the highest altitudes). We found that the maximum electron density relaxation time (> 15000 s) occur between 80 km and 82 km while it decreases with increasing altitudes to 12000 s (at 85 km) and about 2000 s (at 92 km). However, LORES are presumably due to VLF scattering from electron density enhancements caused by lightning-induced EMPs in the uppermost D region ionosphere (85 - 92 km). Thus the observed VLF signal recoveries (LORE recovery times) should associate with the relaxation of the maximum enhanced electron densities produced by elves between 85 km and 92 km [3]. Finally, our results for the lowest altitudes considered (76 km and 77 km) are in good agreement with the recovery times (between 20 s and 120 s) of the typical

  11. 23-gauge vitrectomy and silicone oil tamponade with and without phacoemulsification in rhegmatogenous retinal detachment.

    Science.gov (United States)

    Ozkan, Seyhan Sonar; Citirik, Mehmet; Beyazyildiz, Emrullah; Beyazyildiz, Ozlem

    2015-01-01

    The aim of this study is to assess clinical outcomes of 23-gauge vitrectomy and silicone oil (SO) tamponade combined with and without phacoemulsification (PE) in rhegmatogenous retinal detachment (RRD). The study included forty eyes of 40 patients that underwent 23-gauge vitrectomy and SO tamponade combined with and without PE. Twenty eyes of 20 cases, of whom underwent 23-gauge vitrectomy and SO tamponade combined with PE were allocated to the group 1. Likewise, 20 eyes of 20 cases that underwent 23-gauge vitrectomy and SO tamponade alone were allocated to the group 2. Best corrected visual acuity (BCVA) between two groups was compared. There was no significant difference in BCVA between the two groups during the 6 months (P = 0.3). Recurrent retinal detachments were observed in 2 cases (10%) in both groups. There was no statistically significant difference between two groups as a point of recurrent retinal detachments (P = 1). We have found higher rates of post-vitrectomy cataract progression (45%) in the eyes with RRD who underwent 23-gauge vitrectomy and SO tamponade. Combined vitrectomy and PE is safe and effective for the patients with RRD.

  12. Dynamical evolution of active detached binaries on log Jo - log M diagram and contact binary formation

    CERN Document Server

    Eker, Z; Bilir, S; Karatas, Y

    2006-01-01

    Orbital angular momentum (Jo), systemic mass (M) and orbital period (P) distributions of chromospherically active binaries (CAB) and W Ursae Majoris (W UMa) systems were investigated. The diagrams of log Jo - log P, log M - log P and log Jo-log M were formed from 119 CAB and 102 W UMa stars. The log Jo-log M diagram is found to be most meaningful in demonstrating dynamical evolution of binary star orbits. A slightly curved borderline (contact border) separating the detached and the contact systems was discovered on the log Jo - log M diagram. Since orbital size (a) and period (P) of binaries are determined by their current Jo, M and mass ratio q, the rates of orbital angular momentum loss (dlog Jo/dt) and mass loss (dlog M/dt) are primary parameters to determine the direction and the speed of the dynamical evolution. A detached system becomes a contact system if its own dynamical evolution enables it to pass the contact border on the log Jo - log M diagram. Evolution of q for a mass loosing detached system is...

  13. Selection of anthracnose resistant common beans using detached leaves in partially controlled environment

    Directory of Open Access Journals (Sweden)

    Alisson Campos Pereira

    2014-08-01

    Full Text Available The objectives of this study were to evaluate the possibility of selecting anthracnose resistant common bean plants using detached primary leaves in partially controlled environment of a greenhouse and identify differences in the reaction of genotypes to anthracnose. The common bean cultivars Ouro Negro, OuroVermelho, ManteigãoFosco 11, Rudá, Rudá-R, VP8, BRSMG Madrepérola, Pérola, MeiaNoite and BRSMG Talismãwere characterizedfor resistance to the races 65, 81 and 453 of Colletotrichum lindemuthianum and the method of detached primary leaves was compared to the method with the traditional inoculation of plants at the phenological stage V2. The lines Rudá, Rudá-R and Pérola were inoculated with the races 65 and 453 of C. lindemuthianum, aiming to assess the rate of coincidence of anthracnose severity by both inoculation methods. In general, the two methods presented similar results for the reaction of the cultivars. The use of detached primary leaves of common bean plants in the partially controlled environment was feasible for selection of plants resistant to anthracnose and has the advantages of low-needed infrastructure and reduction of resources, space and time.

  14. Bilateral patching in retinal detachment: fluid mechanics and retinal "settling".

    Science.gov (United States)

    Foster, William J

    2011-07-20

    When a patient suffers a retinal detachment and surgery is delayed, it is known clinically that bilaterally patching the patient may allow the retina to partially reattach or "settle." Although this procedure has been performed since the 1860s, there is still debate as to how such a maneuver facilitates the reattachment of the retina. Finite element calculations using commercially available analysis software are used to elucidate the influence of reduction in eye movement caused by bilateral patching on the flow of subretinal fluid in a physical model of retinal detachment. It was found that by coupling fluid mechanics with structural mechanics, a physically consistent explanation of increased retinal detachment with eye movements can be found in the case of traction on the retinal hole. Large eye movements increase vitreous traction and detachment forces on the edge of the retinal hole, creating a subretinal vacuum and facilitating increased subretinal fluid. Alternative models, in which intraocular fluid flow is redirected into the subretinal space, are not consistent with these simulations. The results of these simulations explain the physical principles behind bilateral patching and provide insight that can be used clinically. In particular, as is known clinically, bilateral patching may facilitate a decrease in the height of a retinal detachment. The results described here provide a description of a physical mechanism underlying this technique. The findings of this study may aid in deciding whether to bilaterally patch patients and in counseling patients on pre- and postoperative care.

  15. Numerical Investigation of Plasma Detachment in Magnetic Nozzle Experiments

    Science.gov (United States)

    Sankaran, Kamesh; Polzin, Kurt A.

    2008-01-01

    At present there exists no generally accepted theoretical model that provides a consistent physical explanation of plasma detachment from an externally-imposed magnetic nozzle. To make progress towards that end, simulation of plasma flow in the magnetic nozzle of an arcjet experiment is performed using a multidimensional numerical simulation tool that includes theoretical models of the various dispersive and dissipative processes present in the plasma. This is an extension of the simulation tool employed in previous work by Sankaran et al. The aim is to compare the computational results with various proposed magnetic nozzle detachment theories to develop an understanding of the physical mechanisms that cause detachment. An applied magnetic field topology is obtained using a magnetostatic field solver (see Fig. I), and this field is superimposed on the time-dependent magnetic field induced in the plasma to provide a self-consistent field description. The applied magnetic field and model geometry match those found in experiments by Kuriki and Okada. This geometry is modeled because there is a substantial amount of experimental data that can be compared to the computational results, allowing for validation of the model. In addition, comparison of the simulation results with the experimentally obtained plasma parameters will provide insight into the mechanisms that lead to plasma detachment, revealing how they scale with different input parameters. Further studies will focus on modeling literature experiments both for the purpose of additional code validation and to extract physical insight regarding the mechanisms driving detachment.

  16. Dynamic displacement of normal and detached semicircular canal cupula.

    Science.gov (United States)

    Rabbitt, Richard D; Breneman, Kathryn D; King, Curtis; Yamauchi, Angela M; Boyle, Richard; Highstein, Stephen M

    2009-12-01

    The dynamic displacement of the semicircular canal cupula and modulation of afferent nerve discharge were measured simultaneously in response to physiological stimuli in vivo. The adaptation time constant(s) of normal cupulae in response to step stimuli averaged 36 s, corresponding to a mechanical lower corner frequency for sinusoidal stimuli of 0.0044 Hz. For stimuli equivalent to 40-200 deg/s of angular head velocity, the displacement gain of the central region of the cupula averaged 53 nm per deg/s. Afferents adapted more rapidly than the cupula, demonstrating the presence of a relaxation process that contributes significantly to the neural representation of angular head motions by the discharge patterns of canal afferent neurons. We also investigated changes in time constants of the cupula and afferents following detachment of the cupula at its apex-mechanical detachment that occurs in response to excessive transcupular endolymph pressure. Detached cupulae exhibited sharply reduced adaptation time constants (300 ms-3 s, n = 3) and can be explained by endolymph flowing rapidly over the apex of the cupula. Partially detached cupulae reattached and normal afferent discharge patterns were recovered 5-7 h following detachment. This regeneration process may have relevance to the recovery of semicircular canal function following head trauma.

  17. Impaired Mucus Detachment Disrupts Mucociliary Transport in a Piglet Model of Cystic Fibrosis

    Science.gov (United States)

    Hoegger, Mark J.; Fischer, Anthony J.; McMenimen, James D.; Ostedgaard, Lynda S.; Tucker, Alex J.; Awadalla, Maged A.; Moninger, Thomas O.; Michalski, Andrew S.; Hoffman, Eric A.; Zabner, Joseph; Stoltz, David A.; Welsh, Michael J.

    2015-01-01

    Lung disease in people with cystic fibrosis (CF) is initiated by defective host defense that predisposes airways to bacterial infection. People with advanced CF exhibit deficits in mucociliary transport (MCT), a process that traps and propels bacteria out of lungs, but whether this occurs first or is secondary to airway remodeling has been unclear. To assess MCT, we tracked movement of radiodense microdisks in airways of newborn CF piglets. Cholinergic stimulation, which elicits mucus secretion, caused microdisks to become stuck. Impaired MCT was not due to periciliary liquid depletion; rather, CF submucosal glands secreted mucus strands that remained tethered to gland ducts and hindered MCT. Inhibiting anion secretion in non-CF airways replicated CF abnormalities. These findings identify impaired MCT as a primary defect, link CFTR loss in submucosal glands to failure of mucus detachment from glands, and suggest that submucosal glands and tethered mucus may be targets for early CF treatment. PMID:25124441

  18. [Vitrectomy in treatment of retinal detachment with proliferative vitreoretinopathy].

    Science.gov (United States)

    Nawrocki, J; Dziegielewski, K; Pikulski, Z

    1993-01-01

    The authors presented their experiences concerning the treatment of 26 eyes (in 25 patients) with retinal detachment complicated by PVR in which pars plana vitrectomy and silicone oil tamponade were applied. The patients have been previously operated with traditional methods however without success. Positive results, it is retinal attachment completely or in the superior and central part of the fundus with residual detachment inferiorly were achieved in 19 eyes (73%). An useful visual acuity (> 1/50) was achieved in 12 eyes and in 7 (27%) it was better than 5/50. Our results confirmed that this method (pars plana vitrectomy with silicone oil tamponade) is a method of choice in complicated retinal detachment, in which traditional methods failed.

  19. [Retinal pneumopexy in the treatment of rhegmatogenous retinal detachment].

    Science.gov (United States)

    Levai, L; Gavriş, Monica; Gábor, Radó; Bagosi, P

    2014-01-01

    To evaluate the efficiency of retinal pneumopexy in patients with rhegmatogenous retinal detachment. This clinical prospective study unrolled between november 2010-june 2012 in the Ophthalmology Department of the Military Hospital in Cluj-Napoca and Satu Mare Emergency Hospital included 20 patients (20 eyes) with rhegmatogenous retinal detachment. Patients were treated with retinal pneumopexy followed by laser photocoagulation. Anatomical and functional results were evaluated 1, 3, 6, 12 and 19 months after treatment. In 17 eyes out of 20, we achieved retinal reattachment and visual recovery. Three cases yelded no success, these being further treated with posterior vitrectomy. Retinal pneumopexy is a minimally invasive treatment method of rhegmatogenous retinal detachment with very good results in well selected cases.

  20. The Detached Point of View in The Great Gatsby

    Institute of Scientific and Technical Information of China (English)

    冯德平

    2008-01-01

    The Great Gatsby is Fitzgerald's finest novel, which was published in 1925, and Eliot considered it "to be the first step that America has taken since Henry James". It is a sensitive and symbolic treatment of themes of contemporary life related with irony and pathos to the legendry of "American dream". In this novel, Fitzgerald used many techniques: the controlled and detached point of view, the crafted structure and symbolism, all these distinguish The Great Gatsby from the style of his earlier works.However, in this paper I want to present my ideas of the detached point of view in The Great Gatsby. During my presentation, I have quoted some materials from critics to support my arguments. In the conclusion part, I also repeat my own opinion: the detached point of view, made The Great Gatsby the "small" masterpiece of American literature.

  1. Management of Post-traumatic Aniridia with Retinal Detachment

    Institute of Scientific and Technical Information of China (English)

    Shibo Tang; Guanting Qiu; Pei Wang; Xiaoling Liang

    2001-01-01

    Purpose: To reconstruct the anatomic and functional impairment in patients with post-traumatic aniridia, aphakia, and retinal detachment. Methods: Four patients with unilateral aniridia and aphakia as well as retinal detachment as results of severe eye injuries underwent scleral buckling, vitrectomy, membrane peeling, endolaser photocoagulation, silicone oil or gas temponade, combined with iris diaphragm-IOL implantation. Results: All four patients achieved successfully anatomic and functional reconstruction after surgery. During five to 22 months postoperative follow-up, all retinas remained attached. The final visual acuity increased from finger counting to 0.1 ~ 0.3. Conclusions: The combination of vitreoretinal surgery and iris diaphrgm-IOL implantation is an effective method for post-traumatic aniridia, aphakia and traumatic retinal detachment. It could ameliorate photophobia and improve the biocular vision.Furthermore, artificial iris diaphragm implantation could prevent silicone oil-endothelia contact and salvage silicone keratopathy. Eye Science 2001; 17:35 ~ 38.

  2. Detached Solidification of Germanium-Silicon Crystals on the ISS

    Science.gov (United States)

    Volz, M. P.; Mazuruk, K.; Croell, A.

    2016-01-01

    A series of Ge(sub 1-x) Si(sub x) crystal growth experiments are planned to be conducted in the Low Gradient Furnace (LGF) onboard the International Space Station. The primary objective of the research is to determine the influence of containment on the processing-induced defects and impurity incorporation in germanium-silicon alloy crystals. A comparison will be made between crystals grown by the normal and "detached" Bridgman methods and the ground-based float zone technique. Crystals grown without being in contact with a container have superior quality to otherwise similar crystals grown in direct contact with a container, especially with respect to impurity incorporation, formation of dislocations, and residual stress in crystals. "Detached" or "dewetted" Bridgman growth is similar to regular Bridgman growth in that most of the melt is in contact with the crucible wall, but the crystal is separated from the wall by a small gap, typically of the order of 10-100 microns. Long duration reduced gravity is essential to test the proposed theory of detached growth. Detached growth requires the establishment of a meniscus between the crystal and the ampoule wall. The existence of this meniscus depends on the ratio of the strength of gravity to capillary forces. On Earth, this ratio is large and stable detached growth can only be obtained over limited conditions. Crystals grown detached on the ground exhibited superior structural quality as evidenced by measurements of etch pit density, synchrotron white beam X-ray topography and double axis X-ray diffraction.

  3. Retinal detachment secondary to ocular perforation during retrobulbar Anaesthesia

    Directory of Open Access Journals (Sweden)

    Gopal Lingam

    1995-01-01

    Full Text Available The clinical characteristics and the retinal breaks associated with rhegmatogenous retinal detachments secondary to accidental globe perforation during local infiltration anaesthesia in five highly myopic eyes are presented. Retinal detachment was total with variable proliferative vitreoretinopathy. The pattern of retinal breaks was rather typical and predictable. Management involved vitreous surgery with internal tamponade by silicone oil in four eyes and perfluoropropane gas in one eye. At the last follow-up, all eyes had attached retina. One eye did not recover useful vision due to possible concurrent optic nerve damage.

  4. Bilateral Simultaneous Rhegmatogenous Retinal Detachment following Laser in situ Keratomileusis

    Directory of Open Access Journals (Sweden)

    Erhan Yumusak

    2016-06-01

    Full Text Available A 21-year-old woman developed simultaneous rhegmatogenous retinal detachment after laser in situ keratomileusis (LASIK in both eyes. She underwent pars plana vitrectomy surgery combined with endolaser photocoagulation and silicone oil tamponade in the right eye. A week later, pneumatic retinopexy was done in the left eye. As the retinal tear did not seal, 360° scleral buckling surgery was performed and retina was attached. Bilateral simultaneous rhegmatogenous retinal detachment after LASIK for correction of myopia can be a serious complication. Patients should be informed about the possibility of this complication.

  5. Calculation of Depth to Detachment and Its Significance in the Kuqa Depression

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The depth to detachment level is a critical factor affecting the quality of structural modeling in fold and thrust belts. There are several detachment levels developed in the Kuqa depression. Based on the excess-area diagram, this paper concerns mainly the calculation of the depth to detachment in the Kuqa depression. The result demonstrates that the detachment levels are situated in different strata in varying zones, such as the Paleogene Kugeliemu Formation, the Paleozoic and the crystalline basement. The calculated depth to detachment level is very helpful for testing whether a structural interpretation is reasonable and for defining the depth of deeper detachment levels which were not discerned in seismic profiles.

  6. Rear Detachments: Capturing and Resourcing How the Army Fights

    Science.gov (United States)

    2012-03-22

    operations/ plans officer), is the Rear Detachment Commander. Captain Basil H. Liddell-Hart was correct in noting that “even the bonds of patriotism...January, 2010; Kyle Moore and Erica Koelder, “Family Readiness in the ARFORGEN Cycle,” Engineer, May/August 2010. 8 Godwin, The Mission, Organization

  7. Spontaneous resolution of optic nerve coloboma-associated retinal detachment.

    Science.gov (United States)

    Ying, Michelle S; Fuller, Jeff; Young, Joshua; Marcus, Dennis M

    2004-01-01

    We report a congenital neurosensory retinal detachment associated with an optic nerve coloboma with subsequent spontaneous reattachment. This represents the earliest reported case of such a clinical situation. An observation period is recommended for infants with this clinical course to allow for the opportunity of spontaneous reattachment.

  8. Liquid Droplet Detachment and Entrainment in Microscale Flows

    Science.gov (United States)

    Hidrovo, Carlos

    2005-11-01

    In this talk we will present a first order study of liquid water detachment and entrainment into air flows in hydrophobic microchannels. Silicon based microstructures consisting of 23 mm long U-shaped channels of different geometry were used for this purpose. The structures are treated with a Molecular Vapor Deposition (MVD) process that renders them hydrophobic. Liquid water is injected through a side slot located 2/3 of the way downstream from the air channel inlet. The water entering the air channel beads up into slugs or droplets that grow in size at this injection location until they fill and flood the channel or are carried away by the air flow. The slugs/droplets dimensions at detachment are correlated against superficial gas velocity and proper dimensionless parameters are postulated and examined to compare hydrodynamic forces against surface tension. It is found that slug/droplet detachment is dominated by two main forces: pressure gradient drag, arising from confinement of a viscous flow in the channel, and inertial drag, arising from the stagnation of the air due to obstruction by the slugs/droplets. A detachment regime map is postulated based on the relative importance of these forces under different flow conditions.

  9. Detached Eddy Simulations of an Airfoil in Turbulent Inflow

    DEFF Research Database (Denmark)

    Gilling, Lasse; Sørensen, Niels; Davidson, Lars

    2009-01-01

    The effect of resolving inflow turbulence in detached eddy simulations of airfoil flows is studied. Synthetic turbulence is used for inflow boundary condition. The generated turbulence fields are shown to decay according to experimental data as they are convected through the domain with the free ...

  10. Existence and Stability of Menisci in Detached Bridgman Growth

    Science.gov (United States)

    Volz, M. P.; Mazuruk, K.

    2011-01-01

    Detached growth, also referred to as dewetted growth, is a Bridgman crystal growth process in which the melt is in contact with the crucible wall but the crystal is not. A meniscus bridges the gap between the top of the crystal and the crucible wall. The Young-Laplace capillary equation was used to calculate the crystal radii of detached states as a function of the pressure differential across the meniscus. The detached states depend on the contact angle of the melt with the crucible wall, the growth angle of the melt with respect to the solidifying crystal, and the Bond number. A static stability analysis was performed on the calculated detached states. The stability criterion was the sign of the second variation of the potential energy upon admissible meniscus shape perturbations. The conditions considered corresponded to the growth of Ge and InSb, in both terrestrial and microgravity conditions. Stability was found to depend significantly on whether the interior surface was considered to be microscopically rough or smooth, corresponding to pinned or unpinned states. It was also found that all meniscus shapes which are single-valued functions of the radius are statically stable in a microgravity environment.

  11. A case of bilateral posterior scleritis with serous detachment

    Directory of Open Access Journals (Sweden)

    Murat Aslankurt, , ,,

    2012-12-01

    Full Text Available Seventeen years old male patient was admitted to theclinic with complaints of pain in the right eye, reductionof vision, painful eye movements and headache for 10days. Visual acuity was found Snellen 0.2 on the right eyeand 1.0 on the left eye. Anterior segment examinationwas normal. Fundus examination revealed bilateral chorioretinalfold on the papillo-macular bundle and serousmacular detachment on the right eye. Fundus angiography(FA revealed late macular hyperfluorescence due toleakage. Exudative retinal detachment on the right maculawas observed in optical coherence tomography (OCT.Orbital ultrasonography and computed tomography wererevealed thickening of the right posterior scleral wall. Investigationsdid not reveal any other cause of headacheor systemic associated autoimmune disease. Oral steroidtreatment (metil prednisolon 64 mg/day was started. Itwas seen that the left eye also affected at the secondweek of treatment. OCT revealed bilateral exudative retinaldetachment. Treatment was going on. Complaint ofdecreased visual acuity was recovered one month later,and bilateral exudative retinal detachment resolved. Inthis article, we aimed to present a case of bilateral idiopathicposterior scleritis and associated exudative retinaldetachment and with good response to therapy.Key words: Posterior scleritis, serous retinal detachment,steroid therapy

  12. Repertoire of NBC The Red Detachment of Women

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    The Red Detachment of Women is a Chinese ballet premiered in 1964.Adapted from the earlier film of the same title, it depicts the liberation of a peasant girl from Hainan Island and her rise in the Communist Party of China.

  13. Commitment and Detachment in English and Bulgarian Academic Writing.

    Science.gov (United States)

    Vassileva, Irena

    2001-01-01

    Examines similarities and differences in the degree of commitment and detachment in English, Bulgarian, and Bulgarian English research articles in linguistics. Analysis shows considerable difference in the overall distribution of hedges and boosters throughout the introductions, discussions, and conclusions of the articles, which may lead to…

  14. Simulating biofilm deformation and detachment with the immersed boundary method

    CERN Document Server

    Sudarsan, Rangarajan; Stockie, John M; Eberl, Hermann J

    2015-01-01

    We apply the immersed boundary (or IB) method to simulate deformation and detachment of a periodic array of wall-bounded biofilm colonies in response to a linear shear flow. The biofilm material is represented as a network of Hookean springs that are placed along the edges of a triangulation of the biofilm region. The interfacial shear stress, lift and drag forces acting on the biofilm colony are computed by using fluid stress jump method developed by Williams, Fauci and Gaver [Disc. Contin. Dyn. Sys. B 11(2):519-540, 2009], with a modified version of their exclusion filter. Our detachment criterion is based on the novel concept of an averaged equivalent continuum stress tensor defined at each IB point in the biofilm which is then used to determine a corresponding von Mises yield stress; wherever this yield stress exceeds a given critical threshold the connections to that node are severed, thereby signalling the onset of a detachment event. In order to capture the deformation and detachment behaviour of a bio...

  15. Characterizing the transition from high recycling to partial detachment

    Science.gov (United States)

    McLean, A. G.; Allen, S. L.; Fenstermacher, M.; Lasnier, C.; Meyer, W. H.; Porter, G.; Soukhanovskii, V.; Bray, B. D.; Carlstrom, T. N.; Leonard, A. W.; Liu, C.; Eldon, D.; Groth, M.; Stangeby, P. C.; Tsui, C.

    2014-10-01

    Experiments at DIII-D have explored the transition from the high recycling to the partially detached divertor condition in L- and H-mode with an unprecedented level of detail. Improved divertor and core Thomson scattering diagnostics were coupled with high resolution spectroscopic studies of molecular and neutral emissions. 2-D Te and ne profiles of the outer leg reveal the earliest indications of formation of the detachment front at the target plate, reducing local Te at the outer strike point from 8-10 eV to 2-3 eV with a marginal ( upstream. These data help guide and expose any missing physics in simulations of detachment onset using state-of-the-art boundary codes, and in predictions for operation with a partial detached divertor in future devices. This work supported in part by the US Department of Energy under DE-AC52-07NA27344 and DE-FC02-04ER54698.

  16. Endovascular therapy of arteriovenous fistulae with electrolytically detachable coils

    Energy Technology Data Exchange (ETDEWEB)

    Jansen, O.; Doerfler, A.; Forsting, M.; Hartmann, M.; Kummer, R. von; Tronnier, V.; Sartor, K. [Dept. of Neuroradiology, University of Heidelberg Medical School (Germany)

    1999-12-01

    We report our experience in using Guglielmi electrolytically detachable coils (GDC) alone or in combination with other materials in the treatment of intracranial or cervical high-flow fistulae. We treated 14 patients with arteriovenous fistulae on brain-supplying vessels - three involving the external carotid or the vertebral artery, five the cavernous sinus and six the dural sinuses - by endovascular occlusion using electrolytically detachable platinum coils. The fistula was caused by trauma in six cases. In one case Ehlers-Danlos syndrome was the underlying disease, and in the remaining seven cases no aetiology could be found. Fistulae of the external carotid and vertebral arteries and caroticocavernous fistulae were reached via the transarterial route, while in all dural fistulae a combined transarterial-transvenous approach was chosen. All fistulae were treated using electrolytically detachable coils. While small fistulae could be occluded with electrolytically detachable coils alone, large fistulae were treated by using coils to build a stable basket for other types of coil or balloons. In 11 of the 14 patients, endovascular treatment resulted in complete occlusion of the fistula; in the remaining three occlusion was subtotal. Symptoms and signs were completely abolished by this treatment in 12 patients and reduced in 2. On clinical and neuroradiological follow-up (mean 16 months) no reappearance of symptoms was recorded. (orig.)

  17. Collisional Detachment of Anions using Fourier Transform Mass Spectrometry

    Science.gov (United States)

    1992-12-01

    fractional horsepower gearmotors ( Bodine Electric Company type NCI-13D3). There are additional specialized valves in the source gas manifolding, which...1-9 (February 1984). 37. Gauyacq, Jean Pierre. Dynamics of Negative Ions. Singapore: World Scientific, 1987. 38. Geltman, S. "Electron Detachment

  18. A xylogalacturonan epitope is specifically associated with plant cell detachment

    DEFF Research Database (Denmark)

    Willats, William George Tycho; McCartney, L.; Steele-King, C.G.

    2004-01-01

    A monoclonal antibody (LM8) was generated with specificity for xyloglacturonan (XGA) isolated from pea (Pisum sativum L.) testae. Characterization of the LM8 epitope indicates that it is a region of XGA that is highly substituted with xylose. Immunocytochemical analysis indicates that this epitope...... that is specifically associated with a plant cell separation process that results in complete cell detachment....

  19. Rings and filaments: The remarkable detached CO shell of U Antliae

    Science.gov (United States)

    Kerschbaum, F.; Maercker, M.; Brunner, M.; Lindqvist, M.; Olofsson, H.; Mecina, M.; De Beck, E.; Groenewegen, M. A. T.; Lagadec, E.; Mohamed, S.; Paladini, C.; Ramstedt, S.; Vlemmings, W. H. T.; Wittkowski, M.

    2017-09-01

    Aims: Our goal is to characterize the intermediate age, detached shell carbon star U Antliae morphologically and physically in order to study the mass-loss evolution after a possible thermal pulse. Methods: High spatial resolution ALMA observations of unprecedented quality in thermal CO lines allow us to derive first critical spatial and temporal scales and constrain modeling efforts to estimate mass-loss rates for both the present day as well as the ejection period of the detached shell. Results: The detached shell is remarkably thin, overall spherically symmetric, and shows a barely resolved filamentary substructure possibly caused by instabilities in the interaction zone of winds with different outflow velocities. The expansion age of the detached shell is of the order of 2700 yr and its overall width indicates a high expansion-velocity and high mass-loss period of only a few hundred years at an average mass-loss rate of ≈10-5 M⊙ yr-1. The post-high-mass-loss-rate-epoch evolution of U Ant shows a significant decline to a substantially lower gas expansion velocity and a mass-loss rate amounting to 4 × 10-8 M⊙ yr-1, at present being consistent with evolutionary changes as predicted for the period between thermal pulses. This paper makes use of the following ALMA data: ADS/JAO.ALMA2015.1.00007.S. ALMA is a partnership of ESO (representing its member states), NSF (USA) and NINS (Japan), together with NRC (Canada), NSC and ASIAA (Taiwan), and KASI (Republic of Korea), in cooperation with the Republic of Chile. The Joint ALMA Observatory is operated by ESO, AUI/NRAO and NAOJ.The reduced ALMA FITS data cubes are available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/605/A116The movie is available at http://www.aanda.org

  20. RESEARCH ON THE RELATFD FACTORS INFLUENCING THE POSITIVE RATE OF SURGICAL INCISION BACTERIAL CULTURE%影响手术切口细菌培养阳性率相关因素研究

    Institute of Scientific and Technical Information of China (English)

    彭燕萍

    2012-01-01

    Objective To study the relative factors which influence the positive rate of bacterial culture from surgical incision, and to improve the accuracy of detection. Methods Retrospectively investigation was used to analysis 1 000 cases of surgical incisions bacterial culture results in our hospital, and find out the related factors which influence the positive rate. Results Several factors were found affecting the bacterial species and positive rate of surgical incision bacterial culture, including the disinfection of patient's skin and doctor's arm, the type of surgical incision, the time of operation, and so on. Conclusion When collecting and culturing the bacteria from surgical incision, we should pay attention to the disinfection of patient's skin and doctor's arm and other influencing factors, and take actions to obtain the accurate culture results.%目的 研究影响手术切口细菌培养阳性率的相关因素,提高检测的准确性.方法 通过回顾性调查方法,对某医院1000例手术患者切口细菌培养结果进行分析,找出相关因素.结果 切口细菌培养的细菌种类及阳性率与病人的皮肤和医生手臂的消毒、手术切口类型、手术时间、手术接台情况等相关因素呈正相关.结论 患者手术切口细菌学标本采集和培养应考虑到手术皮肤和术者手消毒及其他因素,采取控制措施,获得准确的培养结果.

  1. Bacterial gastroenteritis

    Science.gov (United States)

    ... most common types of bacterial gastroenteritis in a couple of days. The goal is to make you feel better and avoid dehydration. Drinking enough fluids and learning what to eat will help ease symptoms. You ...

  2. Bacterial vaginosis

    National Research Council Canada - National Science Library

    Islam, Aliya; Safdar, Anjum; Malik, Ayesha

    2009-01-01

    To estimate the frequency of bacterial vaginosis in women with preterm labour. Descriptive cross sectional study carried out in department of Obstetrics and Gynaecology, Military Hospital and Army Medical College Laboratory, Rawalpindi...

  3. Eukaryotic behaviour of a prokaryotic energy-transducing membrane: fully detached vesicular organelles arise by budding from the Rhodobacter sphaeroides intracytoplasmic photosynthetic membrane.

    Science.gov (United States)

    Niederman, Robert A

    2010-05-01

    A major feature that distinguishes prokaryotic organisms from eukaryotes is their less complex internal structure, in which all membrane-associated functions are thought to be present within a continuous lipid-protein bilayer, rather than with distinct organelles. Contrary to this notion, as described by Tucker and co-workers in this issue of Molecular Microbiology, the application of cryo-electron tomography to the purple bacterium Rhodobacter sphaeroides has demonstrated a heretofore unrecognized ultrastructural complexity within the intracytoplasmic membrane (ICM) housing the photosynthetic apparatus. In addition to distinguishing invaginations of the cytoplasmic membrane (CM) and interconnected vesicular structures still attached to the CM, a eukaryote-like ICM budding process was revealed, which results in the formation of fully detached vesicular structures. These bacterial organelles are able to carry out both the light-harvesting and light-driven energy transduction activities necessary for the cells to assume a photosynthetic lifestyle. Their formation is shown to represent the final stage in a membrane invagination and growth process, originating with small CM indentations, which after cell disruption give rise to a membrane fraction that can be separated from mature ICM vesicles by rate-zone sedimentation.

  4. Being engaged at work and detached at home: A week-level study on work engagement, psychological detachment, and affect

    NARCIS (Netherlands)

    Sonnentag, S.E.; Mojza, E.J.; Binnewies, C.; Scholl, A.

    2008-01-01

    Although earlier research has shown that work engagement is associated with positive outcomes for the employee and the organization, this paper suggests that employees also need time periods for temporarily disengaging (i.e., psychological detaching) from work. We hypothesized that work engagement a

  5. Scleral depressed vitreous shaving, 360 laser, and perfluoropropane (C 3 F 8 for retinal detachment

    Directory of Open Access Journals (Sweden)

    Vivek Chaturvedi

    2014-01-01

    Full Text Available Purpose : To review the characteristics and outcomes of patients who underwent pars plana vitrectomy (PPV with scleral depressed vitreous shaving, 360 degree peripheral endolaser, and 14% C3F8 gas for rhegmatogenous retinal detachment (RRD. Materials and Methods : A retrospective review of a consecutive series of patients who underwent primary repair of RRD by PPV with scleral depressed vitreous shaving, 360 degree peripheral endolaser, and 14% perfluoropropane (C 3 F 8 was conducted. Patients with less than 3 months follow-up, previous retinal surgery, and higher than grade B proliferative vitreoretinopathy were excluded. Results : Ninety-one eyes were included in the study. The mean age was 60.1 years. The mean follow-up was 13.7 months. The macula was detached in 63% (58/91 of the eyes. The reattachment rate after one surgical procedure was 95% (86/91 while overall reattachment rate was 100%. There was no statistically significant difference between reattachment rates of superior, nasal/temporal, or inferior RRDs. The mean final best corrected visual acuity (BCVA was 20/40. Of all the patients, 66% of patients with macula-off RRDs had a final BCVA of 20/40 or better. Conclusions: PPV with scleral depressed vitreous shaving, 360 degree peripheral endolaser, and 14% C 3 F 8 leads to successful anatomical reattachment with visual improvement in patients with primary RRD.

  6. Syphilis presenting as retinal detachment and orchitis in a young man with HIV.

    Science.gov (United States)

    Yogo, Norihiro; Nichol, Aran Cunningham; Campbell, Thomas B; Erlandson, Kristine M

    2014-02-01

    Retinal detachment and testicular lesions are 2 rare presentations of syphilis. We describe a man with bilateral retinal detachment from ocular syphilis and syphilitic orchitis as a manifestation of syphilis and HIV coinfection.

  7. Screening for anthracnose disease resistance in strawberry using a detached leaf assay

    Science.gov (United States)

    Inoculation of detached strawberry leaves with Colletotrichum species may provide a rapid, non-destructive method of identifying anthracnose resistant germplasm. The reliability and validity of assessing disease severity is critical to disease management decisions. We inoculated detached strawberr...

  8. Biofilm Formation and Detachment in Gram-Negative Pathogens Is Modulated by Select Bile Acids.

    Science.gov (United States)

    Sanchez, Laura M; Cheng, Andrew T; Warner, Christopher J A; Townsley, Loni; Peach, Kelly C; Navarro, Gabriel; Shikuma, Nicholas J; Bray, Walter M; Riener, Romina M; Yildiz, Fitnat H; Linington, Roger G

    2016-01-01

    Biofilms are a ubiquitous feature of microbial community structure in both natural and host environments; they enhance transmission and infectivity of pathogens and provide protection from human defense mechanisms and antibiotics. However, few natural products are known that impact biofilm formation or persistence for either environmental or pathogenic bacteria. Using the combination of a novel natural products library from the fish microbiome and an image-based screen for biofilm inhibition, we describe the identification of taurine-conjugated bile acids as inhibitors of biofilm formation against both Vibrio cholerae and Pseudomonas aeruginosa. Taurocholic acid (1) was isolated from the fermentation broth of the fish microbiome-derived strain of Rhodococcus erythropolis and identified using standard NMR and MS methods. Screening of the twelve predominant human steroidal bile acid components revealed that a subset of these compounds can inhibit biofilm formation, induce detachment of preformed biofilms under static conditions, and that these compounds display distinct structure-activity relationships against V. cholerae and P. aeruginosa. Our findings highlight the significance of distinct bile acid components in the regulation of biofilm formation and dispersion in two different clinically relevant bacterial pathogens, and suggest that the bile acids, which are endogenous mammalian metabolites used to solubilize dietary fats, may also play a role in maintaining host health against bacterial infection.

  9. Biofilm Formation and Detachment in Gram-Negative Pathogens Is Modulated by Select Bile Acids.

    Directory of Open Access Journals (Sweden)

    Laura M Sanchez

    Full Text Available Biofilms are a ubiquitous feature of microbial community structure in both natural and host environments; they enhance transmission and infectivity of pathogens and provide protection from human defense mechanisms and antibiotics. However, few natural products are known that impact biofilm formation or persistence for either environmental or pathogenic bacteria. Using the combination of a novel natural products library from the fish microbiome and an image-based screen for biofilm inhibition, we describe the identification of taurine-conjugated bile acids as inhibitors of biofilm formation against both Vibrio cholerae and Pseudomonas aeruginosa. Taurocholic acid (1 was isolated from the fermentation broth of the fish microbiome-derived strain of Rhodococcus erythropolis and identified using standard NMR and MS methods. Screening of the twelve predominant human steroidal bile acid components revealed that a subset of these compounds can inhibit biofilm formation, induce detachment of preformed biofilms under static conditions, and that these compounds display distinct structure-activity relationships against V. cholerae and P. aeruginosa. Our findings highlight the significance of distinct bile acid components in the regulation of biofilm formation and dispersion in two different clinically relevant bacterial pathogens, and suggest that the bile acids, which are endogenous mammalian metabolites used to solubilize dietary fats, may also play a role in maintaining host health against bacterial infection.

  10. Association between oral fluoroquinolone use and the development of retinal detachment: a systematic review and meta-analysis of observational studies.

    Science.gov (United States)

    Chui, Celine S L; Wong, Ian C K; Wong, Lisa Y L; Chan, Esther W

    2015-04-01

    Several observational studies have been published investigating the association between oral fluoroquinolone use and the development of retinal detachment; however, the findings are not concordant. This study is a meta-analysis of the existing literature and estimates the overall absolute risk of such an event. Electronic databases were searched for observational studies on the association between oral fluoroquinolone and retinal detachment up to August 2014. Studies that did not meet the criteria for meta-analysis were narratively reviewed. Cases of retinal detachment during current fluoroquinolone use were also extracted for absolute risk calculation. Seven observational studies were included. Three (case-control and self-controlled case series studies) were eligible for meta-analysis and four (cohort studies) were narratively reviewed. The rate ratio of the case-control studies was 1.82 (95% CI 0.67-4.93), I(2) =96% and the incidence rate ratio of the self-controlled case series was 1.03 (95% CI 0.84-1.27), I(2) =36%. Three of the four cohort studies found no significant association between oral fluoroquinolone use and the development of retinal detachment. The pooled absolute risk of retinal detachment whilst on current oral fluoroquinolone treatment is estimated to be 4.85 per 1000000 prescriptions (95% CI 0.78-8.91). The findings of this systematic review and meta-analysis do not support an association between oral fluoroquinolone use and the development of retinal detachment. Given the low absolute risk, such an event would be rare if there were an association. The current prescribing practice for fluoroquinolones should not be altered because of a previously suggested potential risk of retinal detachment. © The Author 2014. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  11. Results of a temporary balloon buckle in the treatment of 500 retinal detachments and a comparison with pneumatic retinopexy.

    Science.gov (United States)

    Kreissig, I; Failer, J; Lincoff, H; Ferrari, F

    1989-04-15

    Five hundred detachments with a single break or a group of breaks close together were treated with parabulbar balloon and cryopexy in Tübingen between November 1980 and September 1986. Follow-up ranged from six to 91 months. Primary attachment was achieved in 466 eyes (93%). Twelve (2.4%) redetached after the balloon was removed. After a second operation, 490 eyes (98%) were attached and after a third, 493 (99%) were attached. The results with the extraocular balloon were compared with results of a similar group of detachments treated with an intraocular gas bubble. The primary attachment rate with the expanding gas operation was 91%; 11% of these eyes redetached. Although the final attachment rate was 99%, the complications after gas were much more frequent and of serious consequence.

  12. Stressing of the New Madrid Seismic Zone by a lower crust detachment fault

    Science.gov (United States)

    Stuart, William D.; Hildenbrand, Thomas G.; Simpson, Robert W.

    1997-12-01

    A new mechanical model for the cause of the New Madrid seismic zone in the central United States is analyzed. The model contains a subhorizontal detachment fault which is assumed to be near the domed top surface of locally thickened anomalous lower crust ("rift pillow"). Regional horizontal compression induces slip on the fault, and the slip creates a stress concentration in the upper crust above the rift pillow dome. In the coseismic stage of the model earthquake cycle, where the three largest magnitude 7-8 earthquakes in 1811-1812 are represented by a single model mainshock on a vertical northeast trending fault, the model mainshock has a moment equivalent to a magnitude 8 event. During the interseismic stage, corresponding to the present time, slip on the detachment fault exerts a right-lateral shear stress on the locked vertical fault whose failure produces the model mainshock. The sense of shear is generally consistent with the overall sense of slip of 1811-1812 and later earthquakes. Predicted rates of horizontal strain at the ground surface are about 10-7 year-1 and are comparable to some observed rates. The model implies that rift pillow geometry is a significant influence on the maximum possible earthquake magnitude.

  13. Highly edge-connected detachments of graphs and digraphs

    DEFF Research Database (Denmark)

    Berg, Alex Rune; Jackson, Bill; Jordan, Tibor

    2003-01-01

    Let G=(V,E) be a graph or digraph and r:V \\to Z+. An r-detachment of G is a graph H obtained by `splitting' each vertex v \\in V into r(v) vertices. The vertices v1,...,vr(v) obtained by splitting v are called the pieces of v in H. Every edge uv \\in E corresponds to an edge of H connecting some...... piece of u to some piece of v. Crispin Nash-Williams gave necessary and sufficient conditions for a graph to have a k-edge-connected r-detachment. He also solved the version where the degrees of all the pieces are specified. In this paper we solve the same problems for directed graphs. We also give...

  14. Highly Edge-Connected Detachments of Graphs and Digraphs

    DEFF Research Database (Denmark)

    Berg, Alex Rune; Jackson, Bill; Jordán, Tibor

    2003-01-01

    Let G = (V,E) be a graph or digraph and r : V → Z+. An r-detachment of G is a graph H obtained by ‘splitting’ each vertex ν ∈ V into r(ν) vertices. The vertices ν1,…,νr(ν) obtained by splitting ν are called the pieces of ν in H. Every edge uν ∈ E corresponds to an edge of H connecting some piece...... of u to some piece of ν. Crispin Nash-Williams [9] gave necessary and sufficient conditions for a graph to have a k-edge-connected r-detachment. He also solved the version where the degrees of all the pieces are specified. In this paper, we solve the same problems for directed graphs. We also give...

  15. Adhesion Force Measurements of Polymer Particles by Detachment Field Method

    Institute of Scientific and Technical Information of China (English)

    Masashi Nagayama; Nobuyasu Sakurai; Tatsuaki Wada; Manabu Takeuchi

    2004-01-01

    The adhesion force distributions of polymer particles to aluminum substrates were measured by the detachment field method. Polymer particles with conducting surface treatment were used for the measurements.Further the conventional detachment field method was modified to be applicable to the adhesion force measurements of a single particle. The adhesion force of the polymer particles increased with an increase in relative humidity. The surface roughness of the substrate influenced the adhesion forces of particles significantly. The influence of the CF4 plasma treatment of the polymer particles and thin layer coating of the substrate surface on the adhesion forces of the polymer particles was also studied, and factors affecting adhesion forces of polymer particles are discussed.

  16. Tamponade in the surgical management of retinal detachment

    Directory of Open Access Journals (Sweden)

    Vaziri K

    2016-03-01

    Full Text Available Kamyar Vaziri, Stephen G Schwartz, Krishna S Kishor, Harry W Flynn Jr Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA Abstract: Despite treatment advances, rhegmatogenous retinal detachment (RD can have poor visual outcomes even with prompt and appropriate therapy. Pars plana vitrectomy is a leading management modality for the treatment of RD. This procedure is generally accompanied by the use of internal tamponade. Various gases and silicone oils may yield beneficial outcomes. Heavy silicone oils have been approved in some European nations but are not available in the USA. Different tamponade agents have unique benefits and risks, and choice of the agent should be individualized according to the characteristics of the patient and RD, as well as perioperative and postoperative factors. Keywords: tamponade, retinal detachment, silicone oil, gas, air, perfluorocarbon liquids

  17. Intellectual property rights and detached human body parts.

    Science.gov (United States)

    Pila, Justine

    2014-01-01

    This paper responds to an invitation by the editors to consider whether the intellectual property (IP) regime suggests an appropriate model for protecting interests in detached human body parts. It begins by outlining the extent of existing IP protection for body parts in Europe, and the relevant strengths and weaknesses of the patent system in that regard. It then considers two further species of IP right of less obvious relevance. The first are the statutory rights of ownership conferred by domestic UK law in respect of employee inventions, and the second are the economic and moral rights recognised by European and international law in respect of authorial works. In the argument made, both of these species of IP right may suggest more appropriate models of sui generis protection for detached human body parts than patent rights because of their capacity better to accommodate the relevant public and private interests in respect of the same.

  18. Unity and Detachment: A Discourse Analysis of Doctoral Supervision

    Directory of Open Access Journals (Sweden)

    Caroline Bradbury-Jones

    2007-12-01

    Full Text Available In this article the authors report on an approach that they used to enhance their understanding of the complex nature of doctoral supervision by analyzing e-mail communication within a supervisory relationship. Although some scholars have discussed research supervision, empirical research on the subject is limited, and the authors found no published attempts to explore doctoral supervision through the analysis of e-mail communication. The authors analyze correspondence between one doctoral student and two supervisors using discourse analysis influenced by the Foucauldian notion of disciplinary power. The findings revealed the discourses of unity and detachment operating throughout the course of the doctoral relationship. The authors suggest that research students might be no less detached from their supervisors on completion of their studies than at the beginning of their relationship and argue that understanding the discourses of doctoral supervision can enhance the quality and successful outcome of the experience.

  19. Treatment of retinal detachments with multiple breaks by pneumatic retinopexy.

    Science.gov (United States)

    McAllister, I L; Zegarra, H; Meyers, S M; Gutman, F A

    1987-07-01

    Pneumatic retinopexy is a recent innovation in the treatment of uncomplicated retinal detachments due to a superior retinal break extending for 30 degrees or less. We describe four patients with retinal detachments involving multiple breaks who were successfully treated by a modification of this technique. One case involved a calculation of the size of the gas bubble required to achieve tamponade of both breaks simultaneously. The gas was then administered in two sequential injections, as the volume was too large to be given in a single dose. In the remaining three cases, the gas bubble was rotated from the position of tamponade for one break into a position where tamponade of the second break or groups of breaks was achieved.

  20. Evaluation of metal trace detachment from dosing pumps using PIXE

    Science.gov (United States)

    Lozano, Omar; Mejia, Jorge; Laloy, Julie; Alpan, Lütfiye; Toussaint, Olivier; Dogné, Jean-Michel; Lucas, Stéphane

    2014-07-01

    Metal trace detachment evaluation is essential for instruments destined for pharmaceutical applications, such as pumps. Particle-Induced X-ray Emission (PIXE) was used to determine and quantify metal traces originated from stainless steel and ceramic dosing pumps. Metal traces were quantified from either distilled water samples or cellulose filters in two tests: a short-term test of 16 h mimicking a daily cycle of a dosing pump for industrial applications, and a long-term test of 9 days evaluating the pump wearing. The main result is that ceramic dosing pumps present lower metal detachment than stainless steel counterparts. Traces of Si and Al were found originating from pieces around the pumps (pipes and joints).

  1. "Aequanimitas" Redux: William Osler on detached concern versus humanistic empathy.

    Science.gov (United States)

    Bryan, Charles S

    2006-01-01

    Recent critics make William Osler "the father of cool detachment" in medicine, largely because of his "Aequanimitas" address emphasizing objectivity and imperturbability. Closer analysis suggests that Osler's aequanimitas resembles more nearly the metriopatheia of later Stoic philosophy than the apatheia of the early Stoics. A previously unpublished memoir clarifies at least in part Osler's motive for teaching control of the "medullary centres" to minimize facial expression: he did not want to frighten patients, who typically had serious illnesses for which he lacked effective therapy. Twenty-first century challenges to medicine as a profession differ substantially from those of Osler's era. Physicians and educators must focus more closely on the tension between detached concern ("competence") and humanistic empathy ("caring") if medicine is to thrive as a learned profession as opposed to a technical service, a commodity to be bought and sold like any other.

  2. [Treatment of serous macular retinal detachment with antihistamines].

    Science.gov (United States)

    Kirschfeld, K

    2015-01-01

    The etiology of retinal detachment in central serous retinopathy (CSR) is unknown; however, three facts are generally accepted: (1) the serous exudate which raises the layers of the receptors/pigment epithelium is formed due to hyperpermeability in the choriocapillaries, (2) patients frequently suffer from headaches and (3) stress promotes the incidence of CSR. A high blood plasma histamine concentration can cause the abovementioned symptoms which suggests that histamine might provoke CSR. Within 1 week after administration of the antihistamine loratadin a considerable reduction in the retinal exudate and restoration of vision were observed. This supports the hypothesis that histamine could be involved in the process of retinal detachment. Further investigations and large scale clinical trials should clarify if this hypothesis can be proved or disproved and whether antihistamines can be used for age-related macular degeneration (AMD).

  3. Evaluation of metal trace detachment from dosing pumps using PIXE

    Energy Technology Data Exchange (ETDEWEB)

    Lozano, Omar, E-mail: omar.lozanogarcia@unamur.be [Research Centre for the Physics of Matter and Radiation (PMR), Namur Nanosafety Centre (NNC), Namur Research Institute for Life Sciences - NARILIS, University of Namur - UNamur, Rue de Bruxelles 61, B-5000 Namur (Belgium); Mejia, Jorge [Research Centre for the Physics of Matter and Radiation (PMR), Namur Nanosafety Centre (NNC), Namur Research Institute for Life Sciences - NARILIS, University of Namur - UNamur, Rue de Bruxelles 61, B-5000 Namur (Belgium); Laloy, Julie; Alpan, Lütfiye [Namur Thrombosis and Hemostasis Centre (NTHC), Namur Nanosafety Centre (NNC), Namur Research Institute for Life Sciences - NARILIS, University of Namur - UNamur, Rue de Bruxelles 61, B-5000 Namur (Belgium); Toussaint, Olivier [Laboratory of Biochemistry and Cellular Biology (URBC), Namur Nanosafety Centre (NNC), Namur Research Institute for Life Sciences - NARILIS, University of Namur - UNamur, Rue de Bruxelles 61, B-5000 Namur (Belgium); Dogné, Jean-Michel [Namur Thrombosis and Hemostasis Centre (NTHC), Namur Nanosafety Centre (NNC), Namur Research Institute for Life Sciences - NARILIS, University of Namur - UNamur, Rue de Bruxelles 61, B-5000 Namur (Belgium); Lucas, Stéphane [Research Centre for the Physics of Matter and Radiation (PMR), Namur Nanosafety Centre (NNC), Namur Research Institute for Life Sciences - NARILIS, University of Namur - UNamur, Rue de Bruxelles 61, B-5000 Namur (Belgium)

    2014-07-15

    Metal trace detachment evaluation is essential for instruments destined for pharmaceutical applications, such as pumps. Particle-Induced X-ray Emission (PIXE) was used to determine and quantify metal traces originated from stainless steel and ceramic dosing pumps. Metal traces were quantified from either distilled water samples or cellulose filters in two tests: a short-term test of 16 h mimicking a daily cycle of a dosing pump for industrial applications, and a long-term test of 9 days evaluating the pump wearing. The main result is that ceramic dosing pumps present lower metal detachment than stainless steel counterparts. Traces of Si and Al were found originating from pieces around the pumps (pipes and joints)

  4. Fruit Detachment and Classification Method for Strawberry Harvesting Robot

    Directory of Open Access Journals (Sweden)

    Guo Feng

    2008-11-01

    Full Text Available Fruit detachment and on-line classification is important for the development of harvesting robot. With the specific requriements of robot used for harvesting strawberries growing on the ground, a fruit detachment and classification method is introduced in this paper. OHTA color spaces based image segmentation algorithm is utilized to extract strawberry from background; Principal inertia axis of binary strawberry blob is calculated to give the pose information of fruit. Strawberry is picked selectively according to its ripeness and classified according to its shape feature. Histogram matching based method for fruit shape judgment is introduced firstly. Experiment results show that this method can achieve 93% accuracy of strawberry's stem detection, 90% above accuracy of ripeness and shape quality judgment on black and white background. With the improvement of harvesting mechanism design, this method has application potential in the field operation.

  5. Fruit Detachment and Classification Method for Strawberry Harvesting Robot

    Directory of Open Access Journals (Sweden)

    Guo Feng

    2008-03-01

    Full Text Available Fruit detachment and on-line classification is important for the development of harvesting robot. With the specific requriements of robot used for harvesting strawberries growing on the ground, a fruit detachment and classification method is introduced in this paper. OHTA color spaces based image segmentation algorithm is utilized to extract strawberry from background; Principal inertia axis of binary strawberry blob is calculated to give the pose information of fruit. Strawberry is picked selectively according to its ripeness and classified according to its shape feature. Histogram matching based method for fruit shape judgment is introduced firstly. Experiment results show that this method can achieve 93% accuracy of strawberry's stem detection, 90% above accuracy of ripeness and shape quality judgment on black and white background. With the improvement of harvesting mechanism design, this method has application potential in the field operation.

  6. Detachment of Conductive Coatings by Pulsed Electromagnetic Field

    OpenAIRE

    Mironov, V.; Tatarinov, A.; Kolbe, M; Gluschenkov, V.

    2016-01-01

    The paper presents results of studies on the detachment of conductive coatings from the metal substrate by pulsed electromagnetic field (PEMF). It is known that at the boundary of a metal substrate and an electrically conductive coating having different electrical conductivities sharp changes of PEMF strength arise. This effect has been used to remove a copper layer from a steel substrate. Experimental studies were carried out in the Riga Technical University (Latvia), West Sax...

  7. Multiples among detached eclipsing binaries from the ASAS catalog

    CERN Document Server

    Hełminiak, K G; Ratajczak, M; Jordán, A; Espinoza, N; Brahm, R; Kambe, E; Ukita, N

    2015-01-01

    For more than three years now we have been conducting a spectroscopic survey of detached eclipsing binaries (DEBs) from the All-Sky Automated Survey (ASAS) database. Thousands of high-resolution spectra of over 300 systems were secured, and used for radial velocity measurements and spectral analysis. In our sample we found a zoo of multiple systems, such as spectroscopic triples and quadruples, visual binaries with eclipsing components, and circumbinary low-mass companions, including sub-stellar-mass candidates

  8. Tamponade in the surgical management of retinal detachment

    OpenAIRE

    Vaziri K; Schwartz SG; Kishor KS; Flynn HW Jr

    2016-01-01

    Kamyar Vaziri, Stephen G Schwartz, Krishna S Kishor, Harry W Flynn Jr Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA Abstract: Despite treatment advances, rhegmatogenous retinal detachment (RD) can have poor visual outcomes even with prompt and appropriate therapy. Pars plana vitrectomy is a leading management modality for the treatment of RD. This procedure is generally accompanied by the use of internal tamp...

  9. Particle detachment from fluid interfaces: theory vs. experiments.

    Science.gov (United States)

    Anachkov, Svetoslav E; Lesov, Ivan; Zanini, Michele; Kralchevsky, Peter A; Denkov, Nikolai D; Isa, Lucio

    2016-09-28

    Microparticle adsorption and self-assembly at fluid interfaces are strongly affected by the particle three-phase contact angle θ. On the single-particle level, θ can be determined by several techniques, including colloidal-probe AFM, the gel-trapping technique (GTT) and the freeze-fracture shadow-casting (FreSCa) method. While GTT and FreSCa provide contact angle distributions measured over many particles, colloidal-probe AFM measures the wettability of an individual (specified) particle attached onto an AFM cantilever. In this paper, we extract θ for smooth microparticles through the analysis of force-distance curves upon particle approach and retraction from the fluid interface. From each retraction curve, we determine: (i) the maximal force, Fmax; (ii) the detachment distance, Dmax; and (iii) the work for quasistatic detachment, W. To relate Fmax, Dmax and W to θ, we developed a detailed theoretical model based on the capillary theory of flotation. The model was validated in three different ways. First, the contact angles, evaluated from Fmax, Dmax and W, are all close in value and were used to calculate the entire force-distance curves upon particle retraction without any adjustable parameters. Second, the model was successfully applied to predict the experimental force-distance curve of a truncated sphere, whose cut is positioned below the point of particle detachment from the interface. Third, our theory was confirmed by the excellent agreement between the particle contact angles obtained from the colloidal-probe AFM data and the ensemble-average contact angles measured by both GTT and FreSCa. Additionally, we devised a very accurate closed-form expression for W (representing the energy barrier for particle detachment), thus extending previous results in the literature.

  10. Characterizing the Outer Divertor Leg Transition to Full Detachment

    Science.gov (United States)

    McLean, A. G.; Allen, S. L.; Fenstermacher, M. E.; Lasnier, C. J.; Meyer, W. H.; Porter, G. D.; Soukhanovskii, V. A.; Bray, B. D.; Carlstrom, T. N.; Leonard, A. W.; Liu, C.; Eldon, D.; Groth, M.; Stangeby, P. C.; Tsui, C. K.

    2013-10-01

    Experiments at DIII-D have explored the transition from an attached to fully detached divertor condition in L- and H-mode with an unprecedented level of detail. Improved divertor Thomson scattering capturing Te operation in future devices. This work supported in part by the US Department of Energy under DE-AC52-07NA27344 and DE-FC02-04ER54698.

  11. Activation of autophagy in photoreceptor necroptosis after experimental retinal detachment

    Institute of Scientific and Technical Information of China (English)

    Kai; Dong; Zi-Cheng; Zhu; Feng-Hua; Wang; Gen-Jie; Ke; Zhang; Yu; Xun; Xu

    2014-01-01

    AIM:To investigate whether photoreceptor necroptosis induced by z-VAD-FMK(pan caspase inhibitor) was involved the activation of autophagy and whether Necrostatin-1, a specific necroptosis inhibitor, could inhibit this induction of autophagy after experimental retinal detachment.METHODS:Experimental retinal detachment models were created in Sprague-Dawley rats by subretinal injection of sodium hyaluronate and subretinal injections of z-VAD-FMK, vehicle or z-VAD-FMK plus Necrostatin-1.Three days after retinal detachment, morphologic changes were observed by transmission electron microscopy. In other animals, retinas were subjected to immunoprecipitation and Western Blotting, then probed with anti-RIP1, phosphoserine, LC-3II or caspase 8antibody.RESULTS:It was proved by immunoprecipitation and western blotting, that photoreceptor necroptosis was mediated by caspase-8 inhibition and receptor interacting protein kinase(RIP1) phosphorylation activation. Transmission electron microscope and western blotting results indicated that photoreceptornecroptosis was involved the LC-3II and autophagosomes induction. We also discovered Necrostatin-1 could inhibit RIP1 phosphorylation and LC-3II induction.CONCLUSION:These data firstly indicate photoreceptor necroptosis is associated with the activation of autophagy. Necrostatin-1 protects photoreceptors from necroptosis and autophagy by down-regulation of RIP1 phosphorylation and LC-3II.

  12. Primary 25-gauge transconjunctival sutureless vitrectomy in pseudophakic retinal detachment

    Directory of Open Access Journals (Sweden)

    Horozoglu Fatih

    2007-01-01

    Full Text Available Aims: There are few reports on 25-gauge transconjunctival sutureless vitrectomy (TSV in cases of pseudophakic retinal detachment. We conducted this study to report the anatomic and functional outcomes of 25-gauge TSV in the treatment of primary pseudophakic retinal detachment (RD. Design: Prospective, interventional case series. Materials and Methods: Fifteen eyes of 15 patients with RD after cataract surgery with phacoemulsification were evaluated. Primary pseudophakic RDs with macular detachment and proliferative vitreoretinopathy Stage B or less were included in the study. Pars plana vitrectomy with the 25-gauge TSV system, perfluorocarbon liquid injection followed by air exchange, endolaser photocoagulation and sulfur hexafluoride gas (20% injection were applied to all eyes. Results: Mean follow-up time was 9.2 months (range, six to 12 months. Retinal reattachment with a single operation was achieved in 93% of eyes and with additional surgery, the retina was reattached in 100% of eyes. Preoperative visual acuity was less than 20/200 in all eyes (range, hand motions to 20/400. Postoperative visual acuity was 20/40 or better in eight eyes (53% and between 20/50 and 20/200 in seven eyes (47%. No severe hypotony was encountered and no sutures were required to close the scleral and conjunctival openings. Postoperative complications were macular pucker in one eye (7% and cystoid macular edema in another eye (7%. Conclusions: Primary 25-gauge TSV system appears to be an effective and safe procedure in the treatment of uncomplicated pseudophakic RD.

  13. Computation of particle detachment from floors due to human walking

    Science.gov (United States)

    Elhadidi, Basman; Khalifa, Ezzat

    2005-11-01

    A computational model for detachment of fine particles due to the unsteady flow under a foot is developed. As the foot approaches the floor, fluid volume is displaced laterally as a wall jet from the perimeter of the contact area at high velocity and acceleration. Unsteady aerodynamic forces on particles attached to the floor are considered. Results show that the jet velocity is ˜40 m/s for a foot idealized as a 15 cm circular disk approaching the floor at 1 m/s with a final gap of 0.8 mm. This velocity is sufficient to detach small particles (1˜μm). The flow accelerates at ˜400 m/s^2 which affects the detachment of larger sized particles (˜100 μm). As the disk is brought to rest, the unsteady jet expands outwards, advecting a vortex ring closely attached to it. At the disk edge, a counter rotating vortex is generated by the sudden deceleration of the disk. Both vortices can play a role in entrainment of the suspended particles in the flowfield. Numerical studies also show that the maximum jet velocity is ˜20 m/s for a simplified foot immediately after heel contact in the stance phase of the gait.

  14. Pars plana vitrectomy for primary rhegmatogenous retinal detachment

    Directory of Open Access Journals (Sweden)

    Stephen G Schwartz

    2008-03-01

    Full Text Available Stephen G Schwartz, Harry W Flynn JrDepartment of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USAAbstract: Pars plana vitrectomy (PPV is growing in popularity for the treatment of primary rhegmatogenous retinal detachment (RD. PPV achieves favorable anatomic and visual outcomes in a wide variety of patients, especially in pseudophakic RD. A growing number of clinical series, both retrospective and prospective, have demonstrated generally comparable outcomes comparing PPV and scleral buckling (SB under a variety of circumstances. The Scleral Buckling Versus Primary Vitrectomy in Rhegmatogenous Retinal Detachment (SPR study is a multicenter, randomized, prospective, controlled clinical trial comparing SB versus PPV. This study should provide useful guidelines in the future. At this time, the choice of SB versus PPV should be based on the characteristics of the RD, the patient as a whole, and the experience and preference of the individual retinal surgeon.Keywords: pars plana vitrectomy, rhegmatogneous retinal detachment, scleral buckling

  15. Detached cataclysmic variables are crossing the orbital period gap

    CERN Document Server

    Zorotovic, Monica; Parsons, Steven G; Gänsicke, Boris T; Hardy, Adam; Agurto-Gangas, Carolina; Gómez-Morán, Ada Nebot; Rebassa-Mansergas, Alberto; Schwope, Axel D

    2016-01-01

    A central hypothesis in the theory of cataclysmic variable (CV) evolution is the need to explain the observed lack of accreting systems in the ~2-3 h orbital period range, known as the period gap. The standard model, disrupted magnetic braking (DMB), reproduces the gap by postulating that CVs transform into inconspicuous detached white dwarf (WD) plus main sequence (MS) systems, which no longer resemble CVs. However, observational evidence for this standard model is currently indirect and thus this scenario has attracted some criticism throughout the last decades. Here we perform a simple but exceptionally strong test of the existence of detached CVs (dCVs). If the theory is correct dCVs should produce a peak in the orbital period distribution of detached close binaries consisting of a WD and an M4-M6 secondary star. We measured six new periods which brings the sample of such binaries with known periods below 10 h to 52 systems. An increase of systems in the ~2-3 h orbital period range is observed. Comparing ...

  16. On the W7-X divertor performance under detached conditions

    Science.gov (United States)

    Feng, Y.; Beidler, C. D.; Geiger, J.; Helander, P.; Hölbe, H.; Maassberg, H.; Turkin, Y.; Reiter, D.; W7-X Team

    2016-12-01

    We present a theoretical/numerical predictive analysis of the performance of the W7-X island divertor under conditions of detachment characterized by intensive radiation. The analysis is based on EMC3-Eirene simulations and the earlier W7-AS experimental and numerical experience. Carbon is employed as a representative radiator. The associated drawbacks, i.e. core contamination and recycling degradation (reduced recycling flux), are evaluated by determining the carbon density at the last closed flux surface (LCFS) and the neutral pressure in the divertor chamber. Optimum conditions are explored in both configuration and plasma parameter space. This study aims to identify the key geometric/magnetic and plasma parameters that affect the performance of detached plasmas in W7-X. Emphasis is placed on what occurs when the islands are enlarged far beyond the maximum size available in W7-AS and whether an island size limit for optimal detachment operation exists, and why. Further issues addressed are the power removal ability of the W7-X edge islands, potentially limiting factors, compatibility between particle and power exhaust, and particle refueling capability of the recycling neutrals.

  17. Farnesol induces cell detachment from established S. epidermidis biofilms.

    Science.gov (United States)

    Cerca, Nuno; Gomes, Fernanda; Bento, Joana C; França, Angela; Rolo, Joana; Miragaia, Maria; Teixeira, Pilar; Oliveira, Rosário

    2013-05-01

    Antibiotic resistance is a serious problem in Staphylococcus epidermidis infections as many clinical isolates of this organism are resistant to up to eight different antibiotics. The increased resistance to conventional antibiotic therapy has lead to the search for new antimicrobial therapeutic agents. Farnesol, an essential oil found in many plants, has been shown to be active against S. epidermidis. Using a type control strain we recently described that although farnesol was not efficient at killing biofilm bacteria, a strong reduction on biofilm biomass was detected, and we hypothesize that farnesol could, somehow, induce biofilm detachment. In this report, to test our hypothesis we used 36 representative clinical strains of S. epidermidis from different geographic locations and characterized them in terms of genetic variability by multilocus sequence typing and staphylococcal chromosome cassette mec. Strains were tested for biofilm formation, and the presence of ica, bhp and aap genes was determined. Stronger biofilms had always the presence of ica operon but often co-harbored bhp and aap genes. Farnesol was then used in biofilm-forming strains, and biofilm detachment was detected in half of the strains tested. Furthermore, we also showed that farnesol inability to kill biofilm bacteria was not the result of the biofilm structure but was related to high cell density. Our results demonstrate, for the first time, that the biomass reduction previously found by us, and many other groups, is the result not of cell killing but instead is the result of biofilm detachment.

  18. Bacterial Ecology

    DEFF Research Database (Denmark)

    Fenchel, Tom

    2011-01-01

    Bacterial ecology is concerned with the interactions between bacteria and their biological and nonbiological environments and with the role of bacteria in biogeochemical element cycling. Many fundamental properties of bacteria are consequences of their small size. Thus, they can efficiently exploit...

  19. Bacterial lipases

    NARCIS (Netherlands)

    Jaeger, Karl-Erich; Ransac, Stéphane; Dijkstra, Bauke W.; Colson, Charles; Heuvel, Margreet van; Misset, Onno

    1994-01-01

    Many different bacterial species produce lipases which hydrolyze esters of glycerol with preferably long-chain fatty acids. They act at the interface generated by a hydrophobic lipid substrate in a hydrophilic aqueous medium. A characteristic property of lipases is called interfacial activation, mea

  20. Bacterial Adhesion & Blocking Bacterial Adhesion

    DEFF Research Database (Denmark)

    Vejborg, Rebecca Munk

    2008-01-01

    tract to the microbial flocs in waste water treatment facilities. Microbial biofilms may however also cause a wide range of industrial and medical problems, and have been implicated in a wide range of persistent infectious diseases, including implantassociated microbial infections. Bacterial adhesion...... is the first committing step in biofilm formation, and has therefore been intensely scrutinized. Much however, still remains elusive. Bacterial adhesion is a highly complex process, which is influenced by a variety of factors. In this thesis, a range of physico-chemical, molecular and environmental parameters......, which influence the transition from a planktonic lifestyle to a sessile lifestyle, have been studied. Protein conditioning film formation was found to influence bacterial adhesion and subsequent biofilm formation considerable, and an aqueous extract of fish muscle tissue was shown to significantly...

  1. Retinal detachment in southwest Ethiopia: a hospital based prospective study.

    Directory of Open Access Journals (Sweden)

    Tsedeke Asaminew

    Full Text Available PURPOSE: The incidence of retinal detachment in Blacks is generally considered to be low though there are few supporting studies in Africa. This study, thus, aimed at describing the clinical profile of patients with retinal detachment in Southwest Ethiopia. METHODS: A hospital-based study was done on all consecutive retinal detachment patients who presented to Jimma University Hospital over six months period. A semi-structured questionnaire was used to collect patients' sociodemographic characteristics and clinical history. Comprehensive anterior and posterior segment eye examinations were done and risk factors were sought for. Statistical tests were considered significant if P < 0.05. RESULTS: A total of 94 eyes of 80 patients (1.5% had retinal detachment (RD and about 69% of patients were symptomatic for over a month before presentation. The mean age was 41.4 years (SD ±16.5. Fourteen patients (17.5% had bilateral RD. At presentation, 61 eyes (64.9% were blind from RD and 11 (13.8% patients were bilaterally blind from RD. Rhegmatogenous RD was seen in 55 eyes (58.5% and tractional RD in 22 eyes (23.4%. The most common risk factors were ocular trauma (32 eyes, 34.0%, myopia (23 eyes, 24.5%, posterior uveitis (13 eyes, 13.8% and diabetic retinopathy (9 eyes, 9.6%. Most retinal breaks (25 eyes, 43.1% were superotemporal and horse-shoe tear was the most common (19 eyes, 20.2%. Macula was off in 77 eyes (81.9% and 38 eyes (69.1% of RRD eyes had grade C proliferative vitreoretinopathy (PVR. Macular status was significantly associated with PVR (P=0.011, and duration of symptoms (RR=1.25, 95%CI: 1.059-1.475, P=0.040. CONCLUSIONS: A significant numbers of patients with ocular problem had retinal detachment, and nearly two third of the patients presented late. Trauma and myopia were the most important risk factors. People should be educated to improve their health seeking behavior and use eye safety precautions to prevent ocular trauma.

  2. Happy, healthy, and productive: the role of detachment from work during nonwork time.

    Science.gov (United States)

    Fritz, Charlotte; Yankelevich, Maya; Zarubin, Anna; Barger, Patricia

    2010-09-01

    Mentally distancing oneself from work during nonwork time can help restore resources lost because of work demands. In this study, we examined possible outcomes of such psychological detachment from work, specifically well-being and job performance. Although employees may need to mentally detach from work to restore their well-being, high levels of detachment may require a longer time to get back into "working mode," which may be negatively associated with job performance. Our results indicate that higher levels of self-reported detachment were associated with higher levels of significant other-reported life satisfaction as well as lower levels of emotional exhaustion. In addition, we found curvilinear relationships between psychological detachment and coworker reported job performance (task performance and proactive behavior). Thus, although high psychological detachment may enhance employee well-being, it seems that medium levels of detachment are most beneficial for job performance.

  3. Thermochronologic constraints on the slip history of the South Tibetan detachment system in the Everest region, southern Tibet

    Science.gov (United States)

    Schultz, Mary Hannah; Hodges, Kip V.; Ehlers, Todd A.; van Soest, Matthijs; Wartho, Jo-Anne

    2017-02-01

    North-dipping, low-angle normal faults of the South Tibetan detachment system (STDS) are tectonically important features of the Himalayan-Tibetan orogenic system. The STDS is best exposed in the N-S-trending Rongbuk Valley in southern Tibet, where the primary strand of the system - the Qomolangma detachment - can be traced down dip from the summit of Everest for a distance of over 30 km. The metamorphic discontinuity across this detachment implies a large net displacement, with previous studies suggesting >200 km of slip. Here we refine those estimates through thermal-kinematic modeling of new (U-Th)/He and 40Ar/39Ar data from deformed footwall leucogranites. While previous studies focused on the early ductile history of deformation along the detachment, our data provide new insights regarding the brittle-ductile to brittle slip history. Thermal modeling results generated with the program QTQt indicate rapid, monotonic cooling from muscovite 40Ar/39Ar closure (ca. 15.4-14.4 Ma at ca. 490 °C) to zircon (U-Th)/He closure (ca. 14.3-11.0 Ma at ca. 200 °C), followed by slower cooling to apatite (U-Th)/He closure at ca. 9-8 Ma (at ca. 70 °C). Although previous work has suggested that ductile slip on the detachment lasted only until ca. 15.6 Ma, thermal-kinematic modeling of our new data suggests that rapid (ca. 3-4 km/Ma) tectonic exhumation by brittle-ductile to brittle fault slip continued to at least ca. 13.0 Ma. Much lower modeled exhumation rates (≤0.5 km/Ma) after ca. 13 Ma are interpreted to reflect erosional denudation rather than tectonic exhumation. Projection of fault-related exhumation rates backward through time suggests total slip of ca. 61 to 289 km on the Qomolangma detachment, with slightly more than a third of that slip occurring under brittle-ductile to brittle conditions.

  4. Identification of Potential Biomarkers for Rhegmatogenous Retinal Detachment Associated with Choroidal Detachment by Vitreous iTRAQ-Based Proteomic Profiling

    Directory of Open Access Journals (Sweden)

    Zhifeng Wu

    2016-12-01

    Full Text Available Rhegmatogenous retinal detachment associated with choroidal detachment (RRDCD is a complicated and serious type of rhegmatogenous retinal detachment (RRD. In this study, we identified differentially expressed proteins in the vitreous humors of RRDCD and RRD using isobaric tags for relative and absolute quantitation (iTRAQ combined with nano-liquid chromatography-electrospray ion trap-mass spectrometry-mass spectrometry (nano-LC-ESI-MS/MS and bioinformatic analysis. Our result shows that 103 differentially expressed proteins, including 54 up-regulated and 49 down-regulated proteins were identified in RRDCD. Gene ontology (GO analysis suggested that most of the differentially expressed proteins were extracellular.The Kyoto encyclopedia of genes and genomes (KEGG pathway analysis suggested that proteins related to complement and coagulation cascades were significantly enriched. iTRAQ-based proteomic profiling reveals that complement and coagulation cascades and inflammation may play important roles in the pathogenesis of RRDCD. This study may provide novel insights into the pathogenesis of RRDCD and offer potential opportunities for the diagnosis and treatment of RRDCD.

  5. Identification of Potential Biomarkers for Rhegmatogenous Retinal Detachment Associated with Choroidal Detachment by Vitreous iTRAQ-Based Proteomic Profiling

    Science.gov (United States)

    Wu, Zhifeng; Ding, Nannan; Yu, Mengxi; Wang, Ke; Luo, Shasha; Zou, Wenjun; Zhou, Ying; Yan, Biao; Jiang, Qin

    2016-01-01

    Rhegmatogenous retinal detachment associated with choroidal detachment (RRDCD) is a complicated and serious type of rhegmatogenous retinal detachment (RRD). In this study, we identified differentially expressed proteins in the vitreous humors of RRDCD and RRD using isobaric tags for relative and absolute quantitation (iTRAQ) combined with nano-liquid chromatography-electrospray ion trap-mass spectrometry-mass spectrometry (nano-LC-ESI-MS/MS) and bioinformatic analysis. Our result shows that 103 differentially expressed proteins, including 54 up-regulated and 49 down-regulated proteins were identified in RRDCD. Gene ontology (GO) analysis suggested that most of the differentially expressed proteins were extracellular.The Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis suggested that proteins related to complement and coagulation cascades were significantly enriched. iTRAQ-based proteomic profiling reveals that complement and coagulation cascades and inflammation may play important roles in the pathogenesis of RRDCD. This study may provide novel insights into the pathogenesis of RRDCD and offer potential opportunities for the diagnosis and treatment of RRDCD. PMID:27941623

  6. Device-associated infection rates, mortality, length of stay and bacterial resistance in intensive care units in Ecuador: International Nosocomial Infection Control Consortium’s findings

    Science.gov (United States)

    Salgado Yepez, Estuardo; Bovera, Maria M; Rosenthal, Victor D; González Flores, Hugo A; Pazmiño, Leonardo; Valencia, Francisco; Alquinga, Nelly; Ramirez, Vanessa; Jara, Edgar; Lascano, Miguel; Delgado, Veronica; Cevallos, Cristian; Santacruz, Gasdali; Pelaéz, Cristian; Zaruma, Celso; Barahona Pinto, Diego

    2017-01-01

    AIM To report the results of the International Nosocomial Infection Control Consortium (INICC) study conducted in Quito, Ecuador. METHODS A device-associated healthcare-acquired infection (DA-HAI) prospective surveillance study conducted from October 2013 to January 2015 in 2 adult intensive care units (ICUs) from 2 hospitals using the United States Centers for Disease Control/National Healthcare Safety Network (CDC/NHSN) definitions and INICC methods. RESULTS We followed 776 ICU patients for 4818 bed-days. The central line-associated bloodstream infection (CLABSI) rate was 6.5 per 1000 central line (CL)-days, the ventilator-associated pneumonia (VAP) rate was 44.3 per 1000 mechanical ventilator (MV)-days, and the catheter-associated urinary tract infection (CAUTI) rate was 5.7 per 1000 urinary catheter (UC)-days. CLABSI and CAUTI rates in our ICUs were similar to INICC rates [4.9 (CLABSI) and 5.3 (CAUTI)] and higher than NHSN rates [0.8 (CLABSI) and 1.3 (CAUTI)] - although device use ratios for CL and UC were higher than INICC and CDC/NSHN’s ratios. By contrast, despite the VAP rate was higher than INICC (16.5) and NHSN’s rates (1.1), MV DUR was lower in our ICUs. Resistance of A. baumannii to imipenem and meropenem was 75.0%, and of Pseudomonas aeruginosa to ciprofloxacin and piperacillin-tazobactam was higher than 72.7%, all them higher than CDC/NHSN rates. Excess length of stay was 7.4 d for patients with CLABSI, 4.8 for patients with VAP and 9.2 for patients CAUTI. Excess crude mortality in ICUs was 30.9% for CLABSI, 14.5% for VAP and 17.6% for CAUTI. CONCLUSION DA-HAI rates in our ICUs from Ecuador are higher than United States CDC/NSHN rates and similar to INICC international rates. PMID:28289522

  7. Dashed line relaxing retinotomy in the management of retinal detachment with anterior proliferative vitreoretinopathy

    Directory of Open Access Journals (Sweden)

    Tsen CL

    2015-04-01

    Full Text Available Chui-Lien Tsen,1 Yu-Harn Horng,1 Shwu-Jiuan Sheu1,2 1Department of Ophthalmology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan; 2School of Medicine, National Yang-Ming University, Taipei, Taiwan Background: We describe the anatomical and functional outcomes of eyes that underwent a modified technique of relaxing retinotomy, dashed line relaxing retinotomy, in the management of retinal detachment with anterior proliferative vitreoretinopathy.Methods: We retrospectively reviewed 54 consecutive eyes in 52 patients who received pars plana vitrectomy with relaxing retinotomy during retinal detachment repair. Perfluorocarbon liquid (PFCL was used as a standard procedure to stabilize the retina during retinotomy to prevent slippage or inversion of the posterior flap. If PFCL was not available due to economic reasons, dashed line relaxing retinotomy was performed instead. Best-corrected visual acuity, slit-lamp biomicroscopy, intraocular pressure measurement, lens status, and fundus examination were analyzed. We excluded patients who were followed up <4 months.Results: Regarding anatomical success rates and visual outcomes, we found no significant differences between patients treated with intraoperative PFCL and those treated with dashed line relaxing retinotomy without PFCL.Conclusion: Compared to the simple and efficient PFCL-assisted relaxing retinotomy, dashed relaxing retinotomy is not the first choice when PFCL is available. Based on our results, this modified technique may offer an alternative in patients with anterior proliferative vitreoretinopathy for whom PFCL is not available. Keywords: perfluorocarbon liquid, PFCL 

  8. Treatment of retinal detachment due to macular hole in high myopia by vitrectomy with posterior sclera reinforcement

    Directory of Open Access Journals (Sweden)

    Hui-Ru Lin

    2015-05-01

    Full Text Available AIM: To evaluate the efficacy of the method of vitrectomy combined with posterior sclera reinforcement for retinal detachment due to macular hole in high myopia.METHODS: From January 2012 to December 2013, in 45 eyes of 45 high myopic patients with retinal detachment due to macular hole, 28 eyes were in group A of vitrectomy with posterior sclera reinforcement and 17 eyes were in group B of vitrectomy. Preoperative examinations included visual acuity, intraocular pressure, indirect ophthalmoscopy and OCT were performed. In follow-up 6 to 12mo, postoperative examinations of visual acuity, OCT were performed and effects of retinal reattachment and macular hole closure were compared between the two groups. RESULTS:(1Postoperative examinations: visual acuity was 1.19±0.39 in group A and 1.51±0.34 in group B. The differences were statistically significant(PP>0.05.(3The macular hole closure rate was 82% in group A and 53% in group B. The differences were statistically significant(PCONCLUSION: The treatment of vitrectomy with posterior sclera reinforcement is safe and feasible, which could improve visual acuity and increase the rate of macular hole closure in treating retinal detachment due to macular hole in high myopia.

  9. [Bacterial vaginosis].

    Science.gov (United States)

    Romero Herrero, Daniel; Andreu Domingo, Antonia

    2016-07-01

    Bacterial vaginosis (BV) is the main cause of vaginal dysbacteriosis in the women during the reproductive age. It is an entity in which many studies have focused for years and which is still open for discussion topics. This is due to the diversity of microorganisms that cause it and therefore, its difficult treatment. Bacterial vaginosis is probably the result of vaginal colonization by complex bacterial communities, many of them non-cultivable and with interdependent metabolism where anaerobic populations most likely play an important role in its pathogenesis. The main symptoms are an increase of vaginal discharge and the unpleasant smell of it. It can lead to serious consequences for women, such as an increased risk of contracting sexually transmitted infections including human immunodeficiency virus and upper genital tract and pregnancy complications. Gram stain is the gold standard for microbiological diagnosis of BV, but can also be diagnosed using the Amsel clinical criteria. It should not be considered a sexually transmitted disease but it is highly related to sex. Recurrence is the main problem of medical treatment. Apart from BV, there are other dysbacteriosis less characterized like aerobic vaginitis of which further studies are coming slowly but are achieving more attention and consensus among specialists.

  10. Multicenter prospective study on device-associated infection rates and bacterial resistance in intensive care units of Venezuela: International Nosocomial Infection Control Consortium (INICC) findings.

    Science.gov (United States)

    Empaire, Gabriel D; Guzman Siritt, Maria E; Rosenthal, Victor D; Pérez, Fernando; Ruiz, Yvis; Díaz, Claudia; Di Silvestre, Gabriela; Salinas, Evelyn; Orozco, Nelva

    2017-01-01

    Device-associated healthcare-acquired infections (DA-HAI) pose a threat to patient safety in the intensive care unit (ICU). A DA-HAI surveillance study was conducted by the International Nosocomial Infection Control Consortium (INICC) in two adult medical/surgical ICUs at two hospitals in Caracas, Venezuela, in different periods from March 2008 to April 2015, using the US Centers for Disease Control and Prevention's National Healthcare Safety Network (CDC/NHSN) definitions and criteria, and INICC methods. We followed 1041 ICU patients for 4632 bed days. Central line-associated bloodstream infection (CLABSI) rate was 5.1 per 1000 central line days, ventilator-associated pneumonia (VAP) rate was 7.2 per 1000 mechanical ventilator days, and catheter-associated urinary tract infection (CAUTI) rate was 3.9 per 1000 urinary catheter days, all similar to or lower than INICC rates (4.9 [CLABSI]; 16.5 [VAP]; 5.3 [CAUTI]), and higher than CDC/NHSN rates (0.8 [CLABSI]; 1.1 [VAP]; and 1.3 [CAUTI]). Device utilization ratios were higher than INICC and CDC/NHSN rates, except for urinary catheter, which was similar to INICC. Extra length of stay was 8 days for patients with CLABSI, 9.6 for VAP and 5.7 days for CAUTI. Additional crude mortality was 3.0% for CLABSI, 4.4% for VAP, and 16.9% for CAUTI. DA-HAI rates in our ICUs are higher than CDC/NSHN's and similar to or lower than INICC international rates. © The Author 2017. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. All rights reserved.For permissions, please e-mail: journals.permissions@oup.com.

  11. The role of partners and children for employees' psychological detachment from work and well-being.

    Science.gov (United States)

    Hahn, Verena C; Dormann, Christian

    2013-01-01

    This study examined the role of partners and children for employees' psychological detachment from work during off-job time. Building on boundary theory, we hypothesized that not only employees' own work-home segmentation preference but also their partners' work-home segmentation preference is associated with employees' psychological detachment. In addition, partners' psychological detachment should influence employees' psychological detachment. We hypothesized that the presence of children in the household moderates partners' influence on employees' psychological detachment. Further, we expected both employees' and their partners' psychological detachment to contribute to employees' well-being. Participants were 114 dual-earner couples who responded to Web-based questionnaires. The hypotheses were tested with multilevel analyses, using the actor-partner interdependence model. Results confirmed our hypotheses. Employees' and their partners' work-home segmentation preferences were associated with employees' psychological detachment. The presence of children moderated the relation between partners' work-home segmentation preference and employees' psychological detachment. The relation was weaker when there were children in the household. Moreover, employees' and their partners' psychological detachment were positively associated. Again, the relation was weaker when there were children in the household. Finally, both employees' and their partners' psychological detachment contributed to employees' well-being.

  12. Outcomes of Retinal Detachment Surgery in Eyes with Chorioretinal Coloboma

    Directory of Open Access Journals (Sweden)

    Alireza Ramezani

    2010-01-01

    Full Text Available Purpose: To report the anatomical and functional outcomes of surgery for retinal detachment associated with chorioretinal colobomas. Methods: In this retrospective interventional case series, 28 eyes of 28 patients (including 18 male subjects who had undergone surgery for retinal detachment associated with chorioretinal colobomas were evaluated regarding the type of intervention, final visual acuity and anatomical outcomes, as well as complications. Cases with less than 3 months of follow-up were excluded. Results: Primary surgery included vitrectomy in 25 (89.3% and scleral buckling in 3 (10.7% eyes. The internal tamponade used in eyes undergoing vitrectomy was silicone oil in 23 (92% eyes and 20% sulfur hexafluoride (SF6 in 2 (8% eyes. Silicone oil was removed in 11 eyes (45.8%. The mean number of operations per eye was 1.57±0.74, mean follow-up was 40±36 months, and the retina remained attached in 26 eyes (92.9% at final follow-up. Mean preoperative visual acuity was 2.33±0.55 (range, 1.15-2.9 logMAR which significantly improved to 1.72±0.9 (range, 0.09-3.1 logMAR postoperatively (P < 0.001, however, final median visual acuity was counting fingers at 2 m. The most common complications were cataracts (100% and ocular hypertension (46.4%. Conclusion: The most prevalent surgical procedure for treatment of retinal detachment associated with chorioretinal coloboma was pars plana vitrectomy and the most frequently used tamponade was silicone oil. Although anatomical success was satisfactory, functional outcomes were not encouraging which reflects the complexity of the condition and associated abnormalities.

  13. E-CONCAVE:Passive Design of Solar Detached House

    Institute of Scientific and Technical Information of China (English)

    ZhengHao Lin; YiQiang Xiao; ZuLue Cao; HanYang Zhang; ShiQi Qian

    2014-01-01

    With current resources shortages and environmental destruction, one of the important methods in building energy saving is to develop passive design and technologies. “E-concave”, which is one of the entries in the Solar Decathlon 2013, has the core concepts of “Saving”, Comfort”, “Unitization” and “Technology”and brings passive strategies (layout, insulation, daylighting, ventilation, material, etc.) into the design. This article aims to explore the utilization of passive design in solar detached houses through the studies on E-concave and tries to be an example to the development of sustainable buildings in China.

  14. The disappearance and reappearance of Titan's detached haze layer

    Science.gov (United States)

    West, Robert; Rannou, Pascal; Lavvas, Panayotis; Seignovert, Benoit; Turtle, Elizabeth P.; Perry, Jason; Ovanessian, Aida; Roy, Mou

    2016-10-01

    Titan's extended haze is a prominent and long-lived feature of the atmosphere that encompasses a rich variety of chemical, dynamical and microphysical processes operating over a wide range of temporal and spatial scales. The so-called 'detached' haze layer is easily seen in high-resolution short-wave (near-UV and blue wavelengths) images and is a consequence of a nearly global (outside of the winter polar hood region) layer depleted in aerosol content. It was first seen near 350 Km altitude in Voyager images (Rages and Pollack, 1983) and later observed by the Cassini ISS cameras (Porco et al., 2005; West et al., 2010) and UV stellar occultation profiles (Koskinen et al. 2011). A series of Cassini images from 2009 to 2010 revealed what appears to be a seasonally related altitude variation with remarkable regularity (comparing the Voyager and Cassini images). The drop in altitude is most rapid at equinox. Here we report on images of the upper haze layer over the period 2012 to early 2016. In the early part of this period the detached haze continued to drop in altitude and disappeared. There was no evidence for it beginning late in 2012 and extending to early 2016 when it was again detected with very low contrast at an altitude near 500 Km. We document this behavior and examine the evolution of the haze as functions of both latitude and time. These new details put additional constraints on models that attempt to account for the existence of the detached layer. Part of this work was done by the Jet Propulsion Laboratory, California Institute of Technology. References: Rages, K., and J. B. Pollack (1983), Vertical distribution of scattering hazes in Titan's upper atmosphere, Icarus, 55, 50-62, doi:10.1016/0019-1035 (83)90049-0; Porco, C. C. et al., Imaging Titan from the Cassini Spacecraft, Nature 434, 159-168 (2005); West, R. A. et al., The evolution of Titans detached haze layer near equinox in 2009", Geophys. Res. Lett. 38, L06204, doi:10.1029/2011GL046843, 2011

  15. Tamponade in the surgical management of retinal detachment.

    Science.gov (United States)

    Vaziri, Kamyar; Schwartz, Stephen G; Kishor, Krishna S; Flynn, Harry W

    2016-01-01

    Despite treatment advances, rhegmatogenous retinal detachment (RD) can have poor visual outcomes even with prompt and appropriate therapy. Pars plana vitrectomy is a leading management modality for the treatment of RD. This procedure is generally accompanied by the use of internal tamponade. Various gases and silicone oils may yield beneficial outcomes. Heavy silicone oils have been approved in some European nations but are not available in the USA. Different tamponade agents have unique benefits and risks, and choice of the agent should be individualized according to the characteristics of the patient and RD, as well as perioperative and postoperative factors.

  16. Proteomic Analysis of Elevated Intraocular Pressure with Retinal Detachment.

    Science.gov (United States)

    Velez, Gabriel; Roybal, C Nathaniel; Binkley, Elaine; Bassuk, Alexander G; Tsang, Stephen H; Mahajan, Vinit B

    2017-04-01

    To report a case of elevated intraocular pressure with retinal detachment. Liquid chromatography and tandem mass spectrometry was performed on the patient aqueous biopsy. Protein levels were analyzed with 1-way analysis of variance (ANOVA) and unbiased clustering. High levels of rod outer segment proteins were not detected, suggesting that this was not a case of Schwartz-Matsuo syndrome. Instead, elevated levels of Hepcidin (HEPC) and Cystatin C (CYTC; candidate biomarkers for primary open angle glaucoma) were detected, suggesting a different, unknown etiology. Molecular diagnoses can differentiate between clinical diagnoses and point to common biomarkers or disease mechanisms.

  17. Tamponade in the surgical management of retinal detachment

    Science.gov (United States)

    Vaziri, Kamyar; Schwartz, Stephen G; Kishor, Krishna S; Flynn, Harry W

    2016-01-01

    Despite treatment advances, rhegmatogenous retinal detachment (RD) can have poor visual outcomes even with prompt and appropriate therapy. Pars plana vitrectomy is a leading management modality for the treatment of RD. This procedure is generally accompanied by the use of internal tamponade. Various gases and silicone oils may yield beneficial outcomes. Heavy silicone oils have been approved in some European nations but are not available in the USA. Different tamponade agents have unique benefits and risks, and choice of the agent should be individualized according to the characteristics of the patient and RD, as well as perioperative and postoperative factors. PMID:27041988

  18. Hypovolemic shock due to giant uterus leiomyoma detachment.

    Science.gov (United States)

    Galani, P; Kapetanakis, S; Papadopoulos, C; Dimitrakopoulou, G; Kosma, L; Lafoyianni, S; Dimitrakova, E; Papathanasiou, J; Fiska, A

    2010-01-01

    Uterine leiomyoma (UL) is the most common benign gynecologic tumor of the reproductive age females. It is symptomatic only in 25% of the patients causing dysmenorrhea, menorrhagia or anemia. In some patients it occurs just as a palpable abdominal mass. In this study, we report a 50-year-old woman with a giant mass, attached to the uterus, which was detached and therefore led to shock due to major hemorrhage. Surgical removal of both the mass and the uterus confirmed the diagnosis of a pedunculated uterine leiomyoma.

  19. Impact of neonatal intensive care bed configuration on rates of late-onset bacterial sepsis and methicillin-resistant Staphylococcus aureus colonization.

    Science.gov (United States)

    Julian, Samuel; Burnham, Carey-Ann D; Sellenriek, Patricia; Shannon, William D; Hamvas, Aaron; Tarr, Phillip I; Warner, Barbara B

    2015-10-01

    Infections cause morbidity and mortality in neonatal intensive care units (NICUs). The association between nursery design and nosocomial infections is unclear. To determine whether rates of colonization by methicillin-resistant Staphylococcus aureus (MRSA), late-onset sepsis, and mortality are reduced in single-patient rooms. DESIGN Retrospective cohort study. NICU in a tertiary referral center. Our NICU is organized into single-patient and open-unit rooms. Clinical data sets including bed location and microbiology results were examined over 29 months. Differences in outcomes between bed configurations were determined by χ2 and Cox regression. All NICU patients. Among 1,823 patients representing 55,166 patient-days, single-patient and open-unit models had similar incidences of MRSA colonization and MRSA colonization-free survival times. Average daily census was associated with MRSA colonization rates only in single-patient rooms (hazard ratio, 1.31; P=.039), whereas hand hygiene compliance on room entry and exit was associated with lower colonization rates independent of bed configuration (hazard ratios, 0.834 and 0.719 per 1% higher compliance, respectively). Late-onset sepsis rates were similar in single-patient and open-unit models as were sepsis-free survival and the combined outcome of sepsis or death. After controlling for demographic, clinical, and unit-based variables, multivariate Cox regression demonstrated that bed configuration had no effect on MRSA colonization, late-onset sepsis, or mortality. MRSA colonization rate was impacted by hand hygiene compliance, regardless of room configuration, whereas average daily census affected only infants in single-patient rooms. Single-patient rooms did not reduce the rates of MRSA colonization, late-onset sepsis, or death.

  20. Impact of neonatal intensive care bed configuration on rates of late-onset bacterial sepsis and methicillin-resistant Staphylococcus aureus colonization

    Science.gov (United States)

    Julian, Samuel; Burnham, Carey-Ann D.; Sellenriek, Patricia; Shannon, William D.; Hamvas, Aaron; Tarr, Phillip I.; Warner, Barbara B.

    2016-01-01

    Objectives Infections cause significant morbidity and mortality in neonatal intensive care units (NICUs). The association between nursery design and nosocomial infections has not been delineated. We hypothesized that rates of colonization by methicillin-resistant Staphylococcus aureus (MRSA), late-onset sepsis, and mortality are reduced in single-patient rooms. Design Retrospective cohort study. Setting NICU in a tertiary referral center. Methods Our NICU is organized into single-patient and open-unit rooms. Clinical datasets including bed location and microbiology results were examined over a 29-month period. Differences in outcomes between bed configurations were determined by Chi-square and Cox regression. Patients All NICU patients. Results Among 1823 patients representing 55,166 patient-days, single-patient and open-unit models had similar incidences of MRSA colonization and MRSA colonization-free survival times. Average daily census was associated with MRSA colonization rates only in single-patient rooms (hazard ratio 1.31, p=0.039), while hand hygiene compliance on room entry and exit was associated with lower colonization rates independent of bed configuration (hazard ratios 0.834 and 0.719 per 1% higher compliance, respectively). Late-onset sepsis rates were similar in single-patient and open-unit models as were sepsis-free survival and the combined outcome of sepsis or death. After controlling for demographic, clinical and unit-based variables, multivariate Cox regression demonstrated that bed configuration had no effect on MRSA colonization, late-onset sepsis, or mortality. Conclusions MRSA colonization rate was impacted by hand hygiene compliance, regardless of room configuration, while average daily census only affected infants in single-patient rooms. Single-patient rooms did not reduce the rates of MRSA colonization, late-onset sepsis or death. PMID:26108888

  1. Rates of bacterial sulfate reduction and their response to experimental temperature changes in coastal sediments of Qi’ao Island, Zhujiang River Estuary in China

    Institute of Scientific and Technical Information of China (English)

    WU Zijun; ZHOU Huaiyang; PENG Xiaotong; LI Jiangtao; CHEN Guangqian

    2014-01-01

    Subtropical sediment cores (QA09-1 and QA12-9) from the coastal zone of Qi’ao Island in the Zhujiang River Estuary were used to determine the rates of sulfate reduction and their response to experimental tempera-ture changes. The depth distribution of the sulfate reduction rates was measured from whole-core incu-bations with radioactive tracer35SO42-, and peaks of 181.19 nmol/(cm3·d) and 107.49 nmol/(cm3·d) were exhibited at stations QA09-1 and QA12-9, respectively. The profiles of the pore water methane and sulfate concentrations demonstrated that anaerobic oxidation of methane occurred in the study area, which result-ed in an increase in the sulfate reduction rate at the base of the sulfate-reducing zone. Meanwhile, the sulfate concentration was not a major limiting factor for controlling the rates of sulfate reduction. In addition, the incubation of the sediment slurries in a block with a temperature gradient showed that the optimum tem-perature for the sulfate reduction reaction was 36°C. The Arrhenius plot was linear from the lowest tempera-ture to the optimum temperature, and the activation energy was at the lower end of the range of previously reported values. The results suggested that the ambient temperature regime of marine environments prob-ably selected for the microbial population with the best-suited physiology for the respective environment.

  2. Aerosols optical properties in Titan's Detached Haze Layer

    Science.gov (United States)

    Seignovert, Benoit; Rannou, Pascal; Lavvas, Panayotis; West, Robert

    2016-10-01

    Titan's Detached Haze Layer (DHL) was first observed in 1983 by Rages and Pollack during the Voyager 2 is a consistent spherical haze feature surrounding Titan's upper atmosphere and detached from the main haze. Since 2005, the Imaging Science Subsystem (ISS) instrument on board the Cassini mission performs a continuous survey of the Titan's atmosphere and confirmed its persistence at 500 km up to the equinox (2009) before its drop and disappearance in 2012 (West et al. 2011). Previous analyses showed, that this layer corresponds to the transition area between small spherical aerosols and large fractal aggregates and play a key role in the aerosols formation in Titan's atmosphere (Rannou et al. 2000, Lavvas et al. 2009, Cours et al. 2011).In this talk we will present the UV photometric analyses based on radiative transfer inversion to retrieve aerosols particles properties in the DHL (bulk and monomer radius and local density) performed on ISS observations taken from 2005 to 2007.References:- Rages and Pollach, Icarus 55 (1983)- West, et al., Icarus 38 (2011)- Rannou, et al., Icarus 147 (2000)- Lavvas, et al., Icarus 201 (2009)- Cours, et al., ApJ Lett. 741 (2015)

  3. Sector mapping method for 3D detached retina visualization.

    Science.gov (United States)

    Zhai, Yi-Ran; Zhao, Yong; Zhong, Jie; Li, Ke; Lu, Cui-Xin; Zhang, Bing

    2016-10-01

    A new sphere-mapping algorithm called sector mapping is introduced to map sector images to the sphere of an eyeball. The proposed sector-mapping algorithm is evaluated and compared with the plane-mapping algorithm adopted in previous work. A simulation that maps an image of concentric circles to the sphere of the eyeball and an analysis of the difference in distance between neighboring points in a plane and sector were used to compare the two mapping algorithms. A three-dimensional model of a whole retina with clear retinal detachment was generated using the Visualization Toolkit software. A comparison of the mapping results shows that the central part of the retina near the optic disc is stretched and its edges are compressed when the plane-mapping algorithm is used. A better mapping result is obtained by the sector-mapping algorithm than by the plane-mapping algorithm in both the simulation results and real clinical retinal detachment three-dimensional reconstruction.

  4. Current understanding of divertor detachment: experiments and modelling

    Energy Technology Data Exchange (ETDEWEB)

    Wischmeier, W; Groth, M; Kallenbach, A; Chankin, A; Coster, D; Dux, R; Herrmann, A; Muller, H; Pugno, R; Reiter, D; Scarabosio, A; Watkins, J; Team, T D; Team, A U

    2008-05-23

    A qualitative as well as quantitative evaluation of experimentally observed plasma parameters in the detached regime proves to be difficult for several tokamaks. A series of ohmic discharges have been performed in ASDEX Upgrade and DIII-D at similar as possible plasma parameters and at different line averaged densities, {bar n}{sub e}. The experimental data represent a set of well diagnosed discharges against which numerical simulations are compared. For the numerical modeling the fluid-code B2.5 coupled to the Monte Carlo neutrals transport code EIRENE is used. Only the combined enhancement of effects, such as geometry, drift terms, neutral conductance, increased radial transport and divertor target composition, explains a significant fraction of the experimentally observed asymmetries of the ion fluxes as a function of {bar n}{sub e} to the inner and outer target plates in ASDEX Upgrade. The relative importance of the mechanisms leading to detachment are different in DIII-D and ASDEX Upgrade.

  5. Macula-Sparing rhegmatogenous retinal detachment: Is emergent surgery necessary?

    Directory of Open Access Journals (Sweden)

    Sasan Mahmoudi

    2016-01-01

    Full Text Available The status of the macula is a significant factor in determining final visual outcomes in rhegmatogenous retinal detachment (RRD and should be considered in the timing of surgical repair. Several studies have shown that macula-involving RRDs attain similar visual and anatomic outcomes when surgery is performed within seven days as compared to emergent surgery (within 24 hours. In contrast, surgery prior to macular detachment in macula-sparing RRDs generally yields the best visual outcomes. In the case of macula-sparing RRDs, it is not clear how long the macula may remain attached, therefore, standard practice dictates emergent surgery. Timing of presentation, examination findings, case complexity, co-existing medical conditions, surgeon expertise, and timing and quality of access to operating facilities and staff, however, should all be considered in determining whether a macula-sparing RRD requires immediate intervention or if equivalent visual and possibly better overall outcomes can be achieved with scheduled surgery within an appropriate time frame.

  6. Searching for heavy photons at Jefferson Lab using detached vertices

    Science.gov (United States)

    Weinstein, Lawrence; Szumila-Vance, Holly; HPS Collaboration Collaboration

    2017-01-01

    The Heavy Photon Search (HPS) experiment in Jefferson Lab Hall B will look for a new U(1) vector boson (called a ``heavy photon'', ``dark photon'', or A') in the mass range from 20 to 600 MeV/c2. This A' could potentially couple to the ordinary photon through kinetic mixing, which would create a coupling to electric charge of α' / α =ɛ2 . If so, then the A' can be produced through electron interactions with a heavy target through a process analogous to bremsstrahlung. The A' could then decay to e+e- . If the coupling ɛ is large, we can directly detect a peak in the e+e- mass spectrum above the dominant QED background. If ɛ is small, then we can look for e+e- events with a detached vertex resulting from A' decays downstream of the production target. In 2015 and 2016 we measured a few beam days each of 1.1 and 2.2 GeV electron scattering from tungsten using a large acceptance forward spectrometer consisting of a silicon vertex tracker located inside a dipole magnet and a lead tungstate electromagnetic calorimeter for triggering. This talk will present the detached vertex A' search, including preliminary results at 1.1 GeV if available.

  7. Effects of biocides on chlorophyll contents of detached basil leaves

    Directory of Open Access Journals (Sweden)

    Titima Arunrangsi

    2013-06-01

    Full Text Available Herbicides and insecticides have been widely and intensively used in agricultural areas worldwide to enhance crop yield. However, many biocides cause serious environmental problems. In addition, the biocides may also have some effects on the treated agricultural crops. To study effects of biocides on chlorophyll content in detached basil leaves, 2,4-D dimethylamine salt (2,4 D-Amine, paraquat, carbosulfan, and azadirachtin, were chosen as representatives of biocide. After applying the chemicals to detached basil leaves overnight in darkness, chlorophyll contents were determined. Only treatment with 2,4 D-Amine resulted in reduction of chlorophyll contents significantly compared to treatment with deionized (DI water. In the case of paraquat and carbosulfan, chlorophyll contents were not significantly changed, while slightly higher chlorophyll contents, compared to DI water, after the treatment with azadirachtin, were observed. The results indicated that 2,4 D-Amine shows an ability to accelerate chlorophyll degradation, but azadirachtin helps to retard chlorophyll degradation, when each biocide is used at the concentration recommended by the manufacturer.

  8. Engagement and detachment: understanding patients' experiences with nursing.

    Science.gov (United States)

    Kralik, D; Koch, T; Wotton, K

    1997-08-01

    This study aimed to understand what post-operative patients perceived was important about the nursing care they had experienced. The participants were nine women recovering from total hip replacement surgery which had been performed in a large public, acute care hospital in south Australia. Participants volunteered to be involved in the study and were interviewed pre- and post-operatively and interviews continued in their home environment following discharge. The study took place during 1995 within a 10-month time frame. Methodological guidance was sought from the phenomenology literature, with the ideas from Husserl and Heideggar providing shape for the interpretive framework. The analysis of data utilized Colaizzi's (1978) seven procedural steps. For the purposes of this paper the authors have selected to focus only on the findings of this study. Two major themes emerged from the conversations with women. Patients described nurses as being engaged or detached with their nursing care. These themes will be explicated in this paper. In the light of these dominant themes the nursing literature around engagement and detachment are examined. The implications for nursing practice are discussed.

  9. Electron detachment from negative ions in a short laser pulse

    Energy Technology Data Exchange (ETDEWEB)

    Shearer, S. F. C.; Smyth, M. C.; Gribakin, G. F. [School of Mathematics and Physics, Queen' s University Belfast, Belfast, BT7 1NN (United Kingdom)

    2011-09-15

    We present an efficient and accurate method to study electron detachment from negative ions by a few-cycle linearly polarized laser pulse. The adiabatic saddle-point method of Gribakin and Kuchiev [Phys. Rev. A 55, 3760 (1997)] is adapted to calculate the transition amplitude for a short laser pulse. Its application to a pulse with N optical cycles produces 2(N+1) saddle points in complex time, which form a characteristic 'smile.' Numerical calculations are performed for H{sup -} in a 5-cycle pulse with frequency 0.0043 a.u. and intensities of 10{sup 10}, 5x10{sup 10}, and 10{sup 11} W/cm{sup 2}, and for various carrier-envelope phases. We determine the spectrum of the photoelectrons as a function of both energy and emission angle, as well as the angle-integrated energy spectra and total detachment probabilities. Our calculations show that the dominant contribution to the transition amplitude is given by 5-6 central saddle points, which correspond to the strongest part of the pulse. We examine the dependence of the photoelectron angular distributions on the carrier-envelope phase and show that measuring such distributions can provide a way of determining this phase.

  10. Glaucoma associated with the management of rhegmatogenous retinal detachment

    Directory of Open Access Journals (Sweden)

    Mangouritsas G

    2013-04-01

    Full Text Available George Mangouritsas, Spyridon Mourtzoukos, Dimitra M Portaliou, Vassilios I Georgopoulos, Anastasia Dimopoulou, Elias Feretis Eye Clinic, General Hospital "Hellenic Red Cross", Athens, Greece Abstract: Transient or permanent elevation of intraocular pressure (IOP is a common complication following vitreoretinal surgery. Usually secondary glaucoma, which develops after scleral buckling procedures, or pars plana vitrectomy for repair of rhegmatogenous retinal detachment, is of multifactorial origin. It is essential, for appropriate management, to detect the cause of outflow obstruction. An exacerbation of preexisting open-angle glaucoma or a steroid-induced elevation of IOP should also be considered. Scleral buckling may be complicated by congestion and anterior rotation of the ciliary body resulting in secondary angle closure, which can usually resolve with medical therapy. The use of intravitreal gases may also induce secondary angle-closure with or without pupillary block. Aspiration of a quantity of the intraocular gas may be indicated. Secondary glaucoma can also develop after intravitreal injection of silicone oil due to pupillary block, inflammation, synechial angle closure, or migration of emulsified silicone oil in the anterior chamber and obstruction of the aqueous outflow pathway. In most eyes medical therapy is successful in controlling IOP; however, silicone oil removal with or without concurrent glaucoma surgery may also be required. Diode laser transscleral cyclophotocoagulation and glaucoma drainage devices constitute useful treatment modalities for long-term IOP control. Cooperation between vitreoretinal and glaucoma specialists is necessary to achieve successful management. Keywords: retinal detachment, intraocular pressure elevation, glaucoma, vitrectomy, intravitreal gas, silicone oil

  11. Microstructural and deformational studies on mylonite in the detachment faults of Yalashangbo dome, North Himalayan domes zone

    Institute of Scientific and Technical Information of China (English)

    ZHANG Bo; ZHANG Jinjiang; GUO Lei; WANG Weiliang

    2005-01-01

    The Yalashangbo dome, located at the eastern end of North Himalayan domes zone, has a geometry and structure similar to those of a metamorphic core complex. Ductile shear zones formed the detachment system around the dome and these zones are composed of garnet-bearing phyllonite, mylonitic schist, mylonitic gneiss and mylonitic granite. Ductile shear fabrics developed well in mylonitic rocks, and penetrative lineation and foliation were formed by stretched quartz and feldspar and preferred orientation of mica. Polar Mohr diagram method is used to calculate the kinematic vorticity numbers of the shear zones in the detachment system. Results indicate that the shear zone is a thinned shear zone (thinning of 23 % )in an extensional setting which underwent a general shear dominated by simple shear. Comparison of the vorticity numbers between the northern and southern flanks of the Yalashangbo dome shows that the dome is an asymmetric system formed by a north-northwest-directed detaching unanimously. Statistical fractal analysis shows that the shapes of dynamically recrystallized quartz grains in the mylonites have characteristics of self-similarity, with fractal numbers ranging from 1.05 to 1.18. From these fractal numbers, the strain rate of the rock was deduced from 10-9.2 S-1 to 10-7.3 S-1, the differential paleao-stress was 13.7-25.6 MPa during the deformation happened at a temperature over 500 ℃. The ductile shear zones in the detachment system around Yalashangbo dome were formed under a high green-schist grade condition or happened simultaneously with the intrusion of granite.

  12. SiO2/TiO2 Nanocomposite Films on Polystyrene for Light-Induced Cell Detachment Application.

    Science.gov (United States)

    Cheng, Zhiguo; Cheng, Kui; Weng, Wenjian

    2017-01-25

    Light-induced cell detachment shows much potential in in vitro cell culture and calls for high-performance light-responsive films. In this study, a smooth and dense SiO2/TiO2 nanocomposite thin film with thickness of around 250 nm was first fabricated on H2O2 treated polystyrene (PS) substrate via a low-temperature sol-gel method. It was observed that the film could well-adhere on the PS surface and the bonding strength became increasingly high with the increase of SiO2 content. The peeling strength and shear strength reached 3.05 and 30.02 MPa, respectively. It was observed the surface of the film could transform into superhydrophilic upon 20 min illumination of ultraviolet with a wavelength of 365 nm (UV365). In cell culture, cells, i.e., NIH3T3 and MC3T3-E1 cells, cultured on SiO2/TiO2 nanocomposite film were easily detached after 10 min of UV365 illumination; the detachment rates reached 90.8% and 88.6%, respectively. Correspondingly, continuous cell sheets with good viability were also easily obtained through the same way. The present work shows that SiO2/TiO2 nanocomposite thin film could be easily prepared on polymeric surface at low temperature. The corresponding film exhibits excellent biocompatibility, high bonding strength, and good light responses. It could be a good candidate for the surface of cell culture utensils with light-induced cell detachment property.

  13. Detached eddy simulation of unsteady cavitation and pressure fluctuation around 3-D NACA66 hydrofoil

    Directory of Open Access Journals (Sweden)

    Zhang De-Sheng

    2015-01-01

    Full Text Available The unsteady cavitating flow and pressure fluctuation around the 3-D NACA66 hydrofoil were simulated and validated based on detached eddy simulation turbulence model and a homogeneous cavitation model. Numerical results show that detached eddy simulation can predict the evolution of cavity inception, sheet cavitation growth, cloud cavitation shedding, and breakup, as well as the pressure fluctuation on the surface of hydrofoil. The sheet cavitation growth, detachment, cloud cavitation shedding are responsible for the features of the pressure fluctuation.

  14. Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in Rabbits

    DEFF Research Database (Denmark)

    Mandal, Nakul; Lewis, Geoffrey P; Fisher, Steven K

    2015-01-01

    of the vitreous following experimental retinal detachment using a comparative proteomic based approach. Materials and Methods. Retinal detachment was created in the right eyes of six New Zealand red pigmented rabbits. Sham surgery was undertaken in five other rabbits that were used as controls. After seven days......, and α-1-antiproteinase F. Conclusions. Proteomic investigation of the rabbit vitreous has identified a set of proteins that help further our understanding of the pathogenesis of rhegmatogenous retinal detachment and its complications....

  15. Influence of indoor air conditions on radon concentration in a detached house.

    Science.gov (United States)

    Akbari, Keramatollah; Mahmoudi, Jafar; Ghanbari, Mahdi

    2013-02-01

    Radon is released from soil and building materials and can accumulate in residential buildings. Breathing radon and radon progeny for extended periods hazardous to health and can lead to lung cancer. Indoor air conditions and ventilation systems strongly influence indoor radon concentrations. This paper focuses on effects of air change rate, indoor temperature and relative humidity on indoor radon concentrations in a one family detached house in Stockholm, Sweden. In this study a heat recovery ventilation system unit was used to control the ventilation rate and a continuous radon monitor (CRM) was used to measure radon levels. FLUENT, a computational fluid dynamics (CFD) software package was used to simulate radon entry into the building and air change rate, indoor temperature and relative humidity effects using a numerical approach. The results from analytical solution, measurements and numerical simulations showed that air change rate, indoor temperature and moisture had significant effects on indoor radon concentration. Increasing air change rate reduces radon level and for a specific air change rate (in this work Ach = 0.5) there was a range of temperature and relative humidity that minimized radon levels. In this case study minimum radon levels were obtained at temperatures between 20 and 22 °C and a relative humidity of 50-60%.

  16. Synkinematic skarns and fluid drainage along detachments: The West Cycladic Detachment System on Serifos Island (Cyclades, Greece) and its related mineralization

    Science.gov (United States)

    Ducoux, M.; Branquet, Y.; Jolivet, L.; Arbaret, L.; Grasemann, B.; Rabillard, A.; Gumiaux, C.; Drufin, S.

    2017-01-01

    Back-arc extension in the Aegean Sea has been accommodated by several large-scale detachments such as the West Cycladic Detachment System (WCDS) in the Oligocene and Miocene. The WCDS especially crops out on Serifos Island (Cyclades) with a synkinematic granodioritic intrusion. As skarns represent metasomatic reactions near the contact between intrusions and the host rocks, they sign the position of the main drains used by fluids. While the mineralogy of the Serifos skarns is well known, geometrical and kinematic relations between the detachments and ore bodies remains poorly studied. This study allows us to distinguish different types of skarn. High Temperature skarns correspond to (1) massive garnet endoskarns, (2) ribbon and ;bubble; garnet-pyroxene endoskarns, (3) garnet-pyroxene cracks exoskarns, whereas medium-temperature pyroxene ± ilvaite bearing skarn breccias within the Meghàlo Livadhi and Kàvos Kiklopas detachments belonging to the WCDS. Our observations show that skarn formation is associated with the activity of detachments and the syntectonic pluton emplacement. Endo and exoskarn deposits formed coeval with the ductile and brittle structures resulting from the activity of the WCDS, such as echelon veins, veins with antithetic shear and boudinaged veins wrapped within sheath folds, with a top-to-the SSW or SW shear senses compatible with the regional kinematics. Some skarn breccias formed within detachment planes far from the contact of the main granodioritic body, attesting for the intense flow of magmatic fluids along these discontinuities. These over-pressurized fluids play a major role in the mechanical behaviour of the detachments and strain localization. The two detachments acting as preferential crustal-scale drains, the pattern of magmatic fluid flows is not centred on the intrusion. In this context, iron-rich skarns and associated primary magnetite deposits have been remobilized during late brittle increments of motion along the detachments

  17. A Rayleigh number based dendrite fragmentation criterion for detachment of solid crystals during solidification

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, Arvind; Dutta, Pradip [Department of Mechanical Engineering, Indian Institute of Science, Bangalore 560012 (India)], E-mail: pradip@mecheng.iisc.ernet.in

    2008-08-07

    Movement of solid crystals in the form of dendrite fragments causes severe macro-segregation in solidified products. Dendrite fragmentation in the developing mushy zone occurs as a result of remelting (causing dissolution) and subsequent breakage of dendritic side arms from the dendritic stalks. An understanding of the mechanisms of dendrite fragmentation is essential for predicting the transport of fragmented solid crystals for possible control of macro-segregation. In this work, a Rayleigh number based fragmentation criterion is developed for detachment of dendrites from the developing mushy zone, which determines the conditions favourable for fragmentation of dendrites. The Rayleigh number, defined in this paper, measures the ratio of the driving buoyancy force for the flow in the mushy zone to the retarding frictional force associated with the permeability of the mush. The criterion developed is a function of the concentration difference, liquid fraction, permeability, growth rate of mushy layer and thermophysical properties of the material.

  18. Variance-corrected Michaelis-Menten equation predicts transient rates of single-enzyme reactions and response times in bacterial gene-regulation

    CERN Document Server

    Pulkkinen, O

    2016-01-01

    Many chemical reactions in biological cells occur at very low concentrations of constituent molecules. Thus, transcriptional gene-regulation is often controlled by poorly expressed transcription-factors, such as E.coli lac repressor with few tens of copies. Here we study the effects of inherent concentration fluctuations of substrate-molecules on the seminal Michaelis-Menten scheme of biochemical reactions. We present a universal correction to the Michaelis-Menten equation for the reaction-rates. The relevance and validity of this correction for enzymatic reactions and intracellular gene-regulation is demonstrated. Our analytical theory and simulation results confirm that the proposed variance-corrected Michaelis-Menten equation predicts the rate of reactions with remarkable accuracy even in the presence of large non-equilibrium concentration fluctuations. The major advantage of our approach is that it involves only the mean and variance of the substrate-molecule concentration. Our theory is therefore accessi...

  19. The rate of second electron transfer to QB(-) in bacterial reaction center of impaired proton delivery shows hydrogen-isotope effect.

    Science.gov (United States)

    Maróti, Ágnes; Wraight, Colin A; Maróti, Péter

    2015-02-01

    The 2nd electron transfer in reaction center of photosynthetic bacterium Rhodobacter sphaeroides is a two step process in which protonation of QB(-) precedes interquinone electron transfer. The thermal activation and pH dependence of the overall rate constants of different RC variants were measured and compared in solvents of water (H2O) and heavy water (D2O). The electron transfer variants where the electron transfer is rate limiting (wild type and M17DN, L210DN and H173EQ mutants) do not show solvent isotope effect and the significant decrease of the rate constant of the second electron transfer in these mutants is due to lowering the operational pKa of QB(-)/QBH: 4.5 (native), 3.9 (L210DN), 3.7 (M17DN) and 3.1 (H173EQ) at pH7. On the other hand, the proton transfer variants where the proton transfer is rate limiting demonstrate solvent isotope effect of pH-independent moderate magnitude (2.11±0.26 (WT+Ni(2+)), 2.16±0.35 (WT+Cd(2+)) and 2.34±0.44 (L210DN/M17DN)) or pH-dependent large magnitude (5.7 at pH4 (L213DN)). Upon deuteration, the free energy and the enthalpy of activation increase in all proton transfer variants by about 1 kcal/mol and the entropy of activation becomes negligible in L210DN/M17DN mutant. The results are interpreted as manifestation of equilibrium and kinetic solvent isotope effects and the structural, energetic and kinetic possibility of alternate proton delivery pathways are discussed.

  20. Diagnostic tools for studying divertor detachment: bolometry, spectroscopy, and thermography for surface heat-flux

    Science.gov (United States)

    Terry, J. L.; Reinke, M. L.

    2017-04-01

    Some of the key aspects of divertor detachment that are addressed by bolometry, impurity spectroscopy, hydrogen spectroscopy, and measurements of divertor target heat-flux are reviewed. Measurement requirements for these diagnostic areas are defined, and brief descriptions of the techniques used for these diagnostics are given. Examples from the literature of measurements using these tools applied to detachment are presented. Feedback control of detachment using some of these diagnostics as the ‘sensors’ is reviewed. Challenges and some future directions for these diagnostics in the context of studying divertor detachment are described.

  1. Static and dynamic characteristics of plasma detachment in gas target scheme

    Energy Technology Data Exchange (ETDEWEB)

    Ezumi, N.; Tanaka, M.; Mori, S.; Ohno, N.; Takagi, M.; Takamura, S. [Nagoya Univ. (Japan); Suzuki, H.

    1997-12-31

    A simulated gas target divertor experiment has been performed to investigate fundamental physics of plasma detachment in the linear plasma device, TPD-I, which has a high heat flux and high density plasma in steady and pulse state. The existence of density threshold for plasma detachment was observed in our experiment. It is found that the electron-ion temperature relaxation process is a key to determine the density dependence of the plasma detachment. Furthermore, the preliminary experimental results for dynamic behavior of the detached plasma for heat pulse plasma are shown. (author)

  2. Vitrectomy for circumscribed choroidal hemangioma with exudative retinal detachment refractory to transpupillary thermotherapy

    Directory of Open Access Journals (Sweden)

    Shukla Dhananjay

    2007-01-01

    Full Text Available We report successful surgical management of a circumscribed choroidal hemangioma with exudative retinal detachment refractory to transpupillary thermotherapy (TTT. A 33-year-old man with symptomatic serous macular detachment in the left eye (Snellen acuity: 20/200 secondary to a paramacular choroidal hemangioma was treated with TTT. The nonresponsive detachment was subsequently managed by vitrectomy, endophotocoagulation and silicon-oil tamponade. It resulted in complete resolution of the tumor and the detachment. Silicon oil was removed at four months. Visual acuity improved to 20/80 by the last follow-up visit at 10 months without any recurrence.

  3. Surface micropattern limits bacterial contamination

    OpenAIRE

    Mann, Ethan E.; Manna, Dipankar; Mettetal, Michael R; May, Rhea M.; Dannemiller, Elisa M; Chung, Kenneth K.; Brennan, Anthony B; Reddy, Shravanthi T

    2014-01-01

    Background Bacterial surface contamination contributes to transmission of nosocomial infections. Chemical cleansers used to control surface contamination are often toxic and incorrectly implemented. Additional non-toxic strategies should be combined with regular cleanings to mitigate risks of human error and further decrease rates of nosocomial infections. The Sharklet micropattern (MP), inspired by shark skin, is an effective tool for reducing bacterial load on surfaces without toxic additiv...

  4. Bacterial Adhesion & Blocking Bacterial Adhesion

    DEFF Research Database (Denmark)

    Vejborg, Rebecca Munk

    2008-01-01

    tract to the microbial flocs in waste water treatment facilities. Microbial biofilms may however also cause a wide range of industrial and medical problems, and have been implicated in a wide range of persistent infectious diseases, including implantassociated microbial infections. Bacterial adhesion...... in the formation of highly complex sessile communities, referred to as biofilms. Such microbial communities are often highly dynamic and heterogeneous in nature. Microbial biofilms are of great importance in a wide range of natural processes and industrial settings, from the commensal flora of the gastrointestinal...

  5. Kinetics of H+ ion binding by the P+QA-state of bacterial photosynthetic reaction centers: rate limitation within the protein.

    Science.gov (United States)

    Maróti, P; Wraight, C A

    1997-07-01

    The kinetics of flash-induced H+ ion binding by isolated reaction centers (RCs) of Rhodobacter sphaeroides, strain R-26, were measured, using pH indicators and conductimetry, in the presence of terbutryn to block electron transfer between the primary and secondary quinones (QA and QB), and in the absence of exogenous electron donors to the oxidized primary donor, P+, i.e., the P+QA-state. Under these conditions, proton binding by RCs is to the protein rather than to any of the cofactors. After light activation to form P+QA-, the kinetics of proton binding were monoexponential at all pH values studied. At neutral pH, the apparent bimolecular rate constant was close to the diffusional limit for proton transfer in aqueous solution (approximately 10(11) M-1 s-1), but increased significantly in the alkaline pH range (e.g., 2 x 10(13) M-1 s-1 at pH 10). The average slope of the pH dependence was -0.4 instead of -1.0, as might be expected for a H+ diffusion-controlled process. High activation energy (0.54 eV at pH 8.0) and weak viscosity dependence showed that H+ ion uptake by RCs is not limited by diffusion. The salt dependence of the H+ ion binding rate and the pK values of the protonatable amino acid residues of the reaction center implicated surface charge influences, and Gouy-Chapman theory provided a workable description of the ionic effects as arising from modulation of the pH at the surface of the RC. Incubation in D2O caused small increases in the pKs of the protonatable groups and a small, pH (pD)-dependent slowing of the binding rate. The salt, pH, temperature, viscosity, and D2O dependences of the proton uptake by RCs in the P+QA- state were accounted for by three considerations: 1) parallel pathways of H+ delivery to the RC, contributing to the observed (net) H+ disappearance; 2) rate limitation of the protonation of target groups within the protein by conformational dynamics; and 3) electrostatic influences of charged groups in the protein, via the surface pH.

  6. Kinetics of H+ ion binding by the P+QA-state of bacterial photosynthetic reaction centers: rate limitation within the protein.

    Science.gov (United States)

    Maróti, P; Wraight, C A

    1997-01-01

    The kinetics of flash-induced H+ ion binding by isolated reaction centers (RCs) of Rhodobacter sphaeroides, strain R-26, were measured, using pH indicators and conductimetry, in the presence of terbutryn to block electron transfer between the primary and secondary quinones (QA and QB), and in the absence of exogenous electron donors to the oxidized primary donor, P+, i.e., the P+QA-state. Under these conditions, proton binding by RCs is to the protein rather than to any of the cofactors. After light activation to form P+QA-, the kinetics of proton binding were monoexponential at all pH values studied. At neutral pH, the apparent bimolecular rate constant was close to the diffusional limit for proton transfer in aqueous solution (approximately 10(11) M-1 s-1), but increased significantly in the alkaline pH range (e.g., 2 x 10(13) M-1 s-1 at pH 10). The average slope of the pH dependence was -0.4 instead of -1.0, as might be expected for a H+ diffusion-controlled process. High activation energy (0.54 eV at pH 8.0) and weak viscosity dependence showed that H+ ion uptake by RCs is not limited by diffusion. The salt dependence of the H+ ion binding rate and the pK values of the protonatable amino acid residues of the reaction center implicated surface charge influences, and Gouy-Chapman theory provided a workable description of the ionic effects as arising from modulation of the pH at the surface of the RC. Incubation in D2O caused small increases in the pKs of the protonatable groups and a small, pH (pD)-dependent slowing of the binding rate. The salt, pH, temperature, viscosity, and D2O dependences of the proton uptake by RCs in the P+QA- state were accounted for by three considerations: 1) parallel pathways of H+ delivery to the RC, contributing to the observed (net) H+ disappearance; 2) rate limitation of the protonation of target groups within the protein by conformational dynamics; and 3) electrostatic influences of charged groups in the protein, via the surface p

  7. Variance-corrected Michaelis-Menten equation predicts transient rates of single-enzyme reactions and response times in bacterial gene-regulation.

    Science.gov (United States)

    Pulkkinen, Otto; Metzler, Ralf

    2015-12-04

    Many chemical reactions in biological cells occur at very low concentrations of constituent molecules. Thus, transcriptional gene-regulation is often controlled by poorly expressed transcription-factors, such as E.coli lac repressor with few tens of copies. Here we study the effects of inherent concentration fluctuations of substrate-molecules on the seminal Michaelis-Menten scheme of biochemical reactions. We present a universal correction to the Michaelis-Menten equation for the reaction-rates. The relevance and validity of this correction for enzymatic reactions and intracellular gene-regulation is demonstrated. Our analytical theory and simulation results confirm that the proposed variance-corrected Michaelis-Menten equation predicts the rate of reactions with remarkable accuracy even in the presence of large non-equilibrium concentration fluctuations. The major advantage of our approach is that it involves only the mean and variance of the substrate-molecule concentration. Our theory is therefore accessible to experiments and not specific to the exact source of the concentration fluctuations.

  8. Outcomes of 23-gauge pars plana vitrectomy in combined scleral buckling and vitrectomy for complex rhegmatogenous retinal detachments

    Directory of Open Access Journals (Sweden)

    Michael R. Petersen

    2011-06-01

    Full Text Available Rhegmatogenous retinal detachments associated with proliferative vitreoretinopathy, giant retinal tears, ocular trauma, proliferative diabetic retinopathy, or necrotizing retinitis are considered more complex than those without these factors. The aim of the current review is to address the surgical outcomes and complications of 23-gauge pars plana vitrectomy with scleral buckling (23GPPV/SB for repair of these complex retinal detachments. This retrospective study involved 54 eyes of 53 patients who underwent 23GPPV/SB between July 2007 and September 2009. Preoperative diagnosis, surgical technique, preoperative and postoperative visual acuities, intraoperative and postoperative complications, and anatomic reattachment rates were examined. Fifty-four eyes of 53 patients were reviewed in this study and indications for surgery varied. Mean logarithm of the minimal angle of resolution (logMAR pre- and post-operative visual acuities were 1.166 (20/293 and 0.780 (20/120, respectively, which led to a statistically significant improvement in logMAR (P=0.0165. Single operation and final reattachment rates were 87% (47 of 54 eyes and 100%, respectively. Postoperative complications included choroidal effusion/hemorrhage (14.8%, 8 of 54 eyes and vitreous hemorrhage (11.1%, 6 of 54 eyes. Other more infrequent complications included hyphema (9.3%, 5 of 54 eyes, hypotony (5.6%, 3 of 54 eyes and ocular hypertension > 35 mmHg (3.7%, 2 of 54 eyes. A total of 31.5% (17 of 54 eyes of patients had a complication in the postoperative time period, but 58.8% of these resolved spontaneously without requiring an intervention. 23GPPV/SB may be considered for complex retinal detachment repair with good anatomic reattachment rates, but with relatively high complication rates.

  9. Bacterial hydrodynamics

    CERN Document Server

    Lauga, Eric

    2015-01-01

    Bacteria predate plants and animals by billions of years. Today, they are the world's smallest cells yet they represent the bulk of the world's biomass, and the main reservoir of nutrients for higher organisms. Most bacteria can move on their own, and the majority of motile bacteria are able to swim in viscous fluids using slender helical appendages called flagella. Low-Reynolds-number hydrodynamics is at the heart of the ability of flagella to generate propulsion at the micron scale. In fact, fluid dynamic forces impact many aspects of bacteriology, ranging from the ability of cells to reorient and search their surroundings to their interactions within mechanically and chemically-complex environments. Using hydrodynamics as an organizing framework, we review the biomechanics of bacterial motility and look ahead to future challenges.

  10. An Assessment of Molten Metal Detachment Hazards During Electron Beam Welding in Space

    Science.gov (United States)

    Fragomeni, James M.; Nunes, Arthur C., Jr.

    1998-01-01

    The safety issue has been raised with regards to potential molten metal detachments from the weld pool and cold filler wire during electron beam welding in space. This investigation was undertaken to evaluate if molten metal could detach and come in contact with astronauts and burn through the fabric of the astronauts' Extravehicular Mobility Unit (EMU) during electron beam welding in space. Molten metal detachments from either the weld/cut substrate or weld wire could present harm to a astronaut if the detachment was to burn through the fabric of the EMU. Theoretical models were developed to predict the possibility and size of the molten metal detachment hazards during the electron beam welding exercises at Low Earth Orbit (LEO). The primary molten metal detachment concerns were those cases of molten metal separation from the metal surface due to metal cutting, weld pool splashing, entrainment and release of molten metal due to filler wire snap-out from the weld puddle, and molten metal accumulation and release from the end of the weld wire. Some possible ways of obtaining molten metal drop detachments would include an impulse force, or bump, to the weld sample, cut surface, or filler wire. Theoretical models were developed for these detachment concerns from principles of impact and kinetic energies, surface tension, drop geometry, surface energies, and particle dynamics. The surface tension represents the force opposing the liquid metal drop from detaching whereas the weight of the liquid metal droplet represents a force that is tending to detach the molten metal drop. Theoretical calculations have indicated that only a small amount of energy is required to detach a liquid metal drop; however, much of the energy of an impact is absorbed in the sample or weld plate before it reaches the metal drop on the cut edge or surface. The tendency for detachment is directly proportional to the weld pool radius and metal density and inversely proportional to the surface

  11. Detached Eddy Simulation of Flap Side-Edge Flow

    Science.gov (United States)

    Balakrishnan, Shankar K.; Shariff, Karim R.

    2016-01-01

    Detached Eddy Simulation (DES) of flap side-edge flow was performed with a wing and half-span flap configuration used in previous experimental and numerical studies. The focus of the study is the unsteady flow features responsible for the production of far-field noise. The simulation was performed at a Reynolds number (based on the main wing chord) of 3.7 million. Reynolds Averaged Navier-Stokes (RANS) simulations were performed as a precursor to the DES. The results of these precursor simulations match previous experimental and RANS results closely. Although the present DES simulations have not reached statistical stationary yet, some unsteady features of the developing flap side-edge flowfield are presented. In the final paper it is expected that statistically stationary results will be presented including comparisons of surface pressure spectra with experimental data.

  12. 3D description of particle detachment from a smooth surface

    Science.gov (United States)

    Liberzon, Alex; Traugott, Hadar; International CollaborationTurbulence Research Collaboration

    2011-11-01

    This study explores the necessary turbulent flow conditions for initial entrainment of particles from smooth beds into zero-mean-shear flow in an oscillating grid chamber as compared to the bounded shear flow created in a lid-driven cavity. The experiments are not designed to fully mimic the real problem of sediment transport but rather identify key mechanisms, utilizing direct observation and quantification of particle motion at the beginning, during and after lift-off. In both turbulent flows, particle image velocimetry (PIV) and three-dimensional particle tracking velocimetry (3D-PTV) are used to determine the properties of turbulent flows and to track the movement of individual particles through the various phases of the resuspension. The combination of the experimental methods allow to correlate in a quantitative manner the flow conditions responsible for rolling, pick-up, detachment and re-entrainment of particles. The study is supported by the Israel Science Foundation under grant 782/08.

  13. Dwarf Detachment and Globular Cluster Formation in Arp 305

    CERN Document Server

    Hancock, M; Struck, C; Giroux, M L; Hurlock, S

    2009-01-01

    Tidal Dwarf Galaxies (TDG), concentrations of interstellar gas and stars in the tidal features of interacting galaxies, have been the subject of much scrutiny. The `smoking gun' that will prove the TDG hypothesis is the discovery of independent dwarf galaxies that are detached from other galaxies, but have clear tidal histories. As part of a search for TDGs we are using GALEX to conduct a large UV imaging survey of interacting galaxies selected from the Arp Atlas. As part of that study, we present a GALEX UV and SDSS and SARA optical study of the gas-rich interacting galaxy pair Arp 305. The GALEX UV data reveal much extended diffuse UV emission and star formation outside the disks including a candidate TDG between the two galaxies. We have used a smooth particle hydrodynamics code to model the interaction and determine the fate of the candidate TDG.

  14. Compatibility of detached divertor operation with robust edge pedestal performance

    Energy Technology Data Exchange (ETDEWEB)

    Leonard, A.W., E-mail: leonard@fusion.gat.com [General Atomics, PO Box 85608, San Diego, CA 92186-5608 (United States); Makowski, M.A.; McLean, A.G. [Lawrence Livermore National Laboratory, Livermore, CA (United States); Osborne, T.H.; Snyder, P.B. [General Atomics, PO Box 85608, San Diego, CA 92186-5608 (United States)

    2015-08-15

    The compatibility of detached radiative divertor operation with a robust H-mode pedestal is examined in DIII-D. A density scan produced low temperature plasmas at the divertor target, T{sub e} ⩽ 2 eV, with high radiation leading to a factor of ⩾4 drop in peak divertor heat flux. The cold radiative plasma was confined to the divertor and did not extend across the separatrix in X-point region. A robust H-mode pedestal was maintained with a small degradation in pedestal pressure at the highest densities. The response of the pedestal pressure to increasing density is reproduced by the EPED pedestal model. However, agreement of the EPED model with experiment at high density requires an assumption of reduced diamagnetic stabilization of edge Peeling–Ballooning modes.

  15. Descemet's membrane detachments post cataract surgery: a management paradigm

    Science.gov (United States)

    Samarawickrama, Chameen; Beltz, Jacqueline; Chan, Elsie

    2016-01-01

    Descemet's membrane detachments (DMD) are relatively common after cataract surgery and most do not require any treatment. However, if large DMD are not treated appropriately, significant visual morbidity can ensue. We aim to develop a guideline for the management of DMD post cataract surgery based on a retrospective review of all cases encountered at the Royal Victorian Eye and Ear Hospital, Melbourne, Australia over a 4-year period from 2010 to 2014. We suggest conservative management if the visual axis is not involved; however, after 3mo surgical intervention may be warranted to prevent corneal sequelae. In cases where the visual axis is involved we suggest early intervention with air tamponade. The main risk factor for irreversible corneal oedema and subsequent endothelial transplant appears to be direct endothelial trauma rather than the DMD itself. PMID:28003989

  16. Scleral buckling biomaterials and implants for retinal detachment surgery.

    Science.gov (United States)

    Baino, Francesco

    2010-11-01

    Scleral buckling is a widely used surgical procedure that aims at repairing retinal detachments. Many materials and procedural techniques have been variously proposed and tested in an attempt to find the best combination for providing optimal results to the patient. This review highlights the evolution of scleral buckling implants and chronicles the main advances that have been made in such a context. Specifically, the limitations of the materials and implants fallen in disuse, as well as the advantages of currently adopted devices are critically examined and discussed. Future directions for the research are considered, underlining in particular the great potential carried by the development of accurate mathematical models for describing the postoperative evolution of buckled eye. These analytical models, supported by a comprehensive data set provided by advanced techniques of medical investigations, may become useful tools for helping surgeons to choose, and to design if necessary, the best buckling material and configuration to be used in each specific clinical case.

  17. SELECTIVE WASTE COLLECTION IN THE DETACHED HOUSING AREA

    Directory of Open Access Journals (Sweden)

    Iwona Pasiecznik

    2015-06-01

    Full Text Available When it comes to waste management, the main objective of the European Union is to prevent waste production. Produced waste should be primarily recycled or other recovery should be applied. Introduction of an effective waste collection system is a basic activity that guarantees achieving European Union agenda including the minimal level of recycling and preparation for reuse. The aim of the research was the analysis of the selective waste collection in the detached housing area (before 1st July 2013. The evaluation was prepared on the basis of an opinion research conducted among the residents. One hundred respondents, living in one of the housing estates in Wrocław, took part in the survey.

  18. Runoff initiation, soil detachment and connectivity are enhanced as a consequence of vineyards plantations.

    Science.gov (United States)

    Cerdà, A; Keesstra, S D; Rodrigo-Comino, J; Novara, A; Pereira, P; Brevik, E; Giménez-Morera, A; Fernández-Raga, M; Pulido, M; di Prima, S; Jordán, A

    2017-11-01

    Rainfall-induced soil erosion is a major threat, especially in agricultural soils. In the Mediterranean belt, vineyards are affected by high soil loss rates, leading to land degradation. Plantation of new vines is carried out after deep ploughing, use of heavy machinery, wheel traffic, and trampling. Those works result in soil physical properties changes and contribute to enhanced runoff rates and increased soil erosion rates. The objective of this paper is to assess the impact of the plantation of vineyards on soil hydrological and erosional response under low frequency - high magnitude rainfall events, the ones that under the Mediterranean climatic conditions trigger extreme soil erosion rates. We determined time to ponding, Tp; time to runoff, Tr; time to runoff outlet, Tro; runoff rate, and soil loss under simulated rainfall (55 mm h(-1), 1 h) at plot scale (0.25 m(2)) to characterize the runoff initiation and sediment detachment. In recent vine plantations (50 years; O). Slope gradient, rock fragment cover, soil surface roughness, bulk density, soil organic matter content, soil water content and plant cover were determined. Plantation of new vineyards largely impacted runoff rates and soil erosion risk at plot scale in the short term. Tp, Tr and Tro were much shorter in R plots. Tr-Tp and Tro-Tr periods were used as connectivity indexes of water flow, and decreased to 77.5 and 33.2% in R plots compared to O plots. Runoff coefficients increased significantly from O (42.94%) to R plots (71.92%) and soil losses were approximately one order of magnitude lower (1.8 and 12.6 Mg ha(-1) h(-1) for O and R plots respectively). Soil surface roughness and bulk density are two key factors that determine the increase in connectivity of flows and sediments in recently planted vineyards. Our results confirm that plantation of new vineyards strongly contributes to runoff initiation and sediment detachment, and those findings confirms that soil erosion control strategies

  19. Grip and detachment of locusts on inverted sandpaper substrates

    Energy Technology Data Exchange (ETDEWEB)

    Han Longbao; Wang Zhouyi; Ji Aihong; Dai Zhendong, E-mail: zddai@nuaa.edu.cn [Institute of Bio-inspired Structure and Surface Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016 (China)

    2011-12-15

    Locusts (Locusta migratoria manilensis) are characterized by their strong flying and grasping ability. Research on the grasping mechanism and behaviour of locusts on sloping substrates plays an important role in elucidating the mechanics of hexapod locomotion. Data on the maximum angles of slope at which locusts can grasp stably (critical angles of detachment) were obtained from high-speed video recordings at 215 fps. The grasping forces were collected by using two sensors, in situations where all left legs were standing on one and the right legs on the other sensor plate. These data were used to illustrate the grasping ability of locusts on slopes with varying levels of roughness. The grasping morphologies of locusts' bodies and tarsi were observed, and the surface roughness as well as diameters of their claw tips was measured under a microscope to account for the grasping mechanism of these insects on the sloping substrate. The results showed that the claw tips and part of the pads were in contact with the inverted substrate when the mean particle diameter was in the range of 15.3-40.5 {mu}m. The interaction between pads and substrates may improve the stability of contact, and claw tips may play a key role in keeping the attachment reliable. A model was developed to explain the significant effects of the relative size of claw tips and mean particle diameter on grasping ability as well as the observed increase in lateral force (2.09-4.05 times greater than the normal force during detachment) with increasing slope angle, which indicates that the lateral force may be extremely important in keeping the contact reliable. This research lays the groundwork for the probable design and development of biomimetic robotics.

  20. Bacterial adhesion and biofilm formation over a substrate with micro printed oily patches

    Science.gov (United States)

    Jalali, Maryam; Sheng, Jian

    2014-11-01

    Over the past few years, there has been a significant focus on the processes involved in biodegradation of crude oil. In prior studies, using soft lithography and surface functionalization, we have fabricated solid substrates with micro-scale chemical patterns, and applied them to studying the bacteria-surface interactions as well as the formation of biofilm over these micro-patterned surfaces. A strong correlation between biofilm morphology and substrate patterns was found. In our current work we investigate the bacterial adhesion and biofilm formation of hydrocarbon degrading bacteria on micro printed oily surfaces with different micro-scale textures. The oily patterns were formed by contact printing of crude oil on a glass substrate with PDMS stamps. The oil patterned surface is additionally combined with a microfluidics as its bottom substrate. This unique lab-on-a-chip device allows us to investigate the complex interactions microscopically and over a long time. Additionally, it allows us to conduct experiments to elucidate the dynamic interactions such as swimming, dispersion, attachment, detachment, and adsorption between bacteria and micro printed oily surfaces under flow conditions in-situ. The growth rates and morphology of bacterial colony and biofilm are also studied and reported.

  1. Five year nationwide incidence of rhegmatogenous retinal detachment requiring surgery in Korea.

    Directory of Open Access Journals (Sweden)

    Sang Jun Park

    Full Text Available PURPOSE: To define the incidence and demographic characteristics of rhegmatogenous retinal detachment (RRD requiring surgery in Korea. DESIGN: Nationwide population-based retrospective study. METHODS: Patients who underwent surgery for RRD from 2007 to 2011 were retrospectively identified using the diagnostic code for RRD and the surgical codes for retinal detachment surgeries in the national claim database. The average incidence rate of RRD during the 5-year period was estimated using the population data of the 2010 Census in Korea. RESULTS: A total of 24,928 surgically treated RRD cases were identified. The average incidence of surgery requiring RRD was 10.39 cases per 100,000 person-years [95% confidence interval (CI, 10.26-10.52. The incidence in men (11.32 cases per 100,000 person-years; 95% CI: 11.13-11.51 was significantly higher than that in women (9.47 cases per 100,000 person-years; 95% CI: 9.29-9.64 (p<0.001. The incidence of surgery requiring RRD showed a bimodal distribution across age groups, with one peak (28.55 cases per 100,000 person-years; 95% CI: 27.46-29.67 representing patients between 65 and 69 years of age and the second peak (approximately 8.5 per 100,000 person-years representing patients between 20 and 29 years of age. The male-to-female ratio was approximately 1.0 for the peak-incidence age groups, whereas the ratio was higher for the other age groups. CONCLUSIONS: The incidence of RRD in the Korean population was similar to that reported previously, with the peak incidence being lower than that in the Caucasian population. The age-specific RRD incidence pattern in Korea followed a bimodal distribution.

  2. Bilateral detachment of the macular neuroepithelium in a patient with Klinefelter syndrome.

    NARCIS (Netherlands)

    Haarlem, J. van; Cruysberg, J.R.M.; Deutman, A.F.

    2003-01-01

    PURPOSE: To describe the occurrence of atypical, bilateral detachment of the macular neuroepithelium and Klinefelter syndrome in a young patient. METHODS: Case report. RESULTS: A 20-year-old male of Chinese origin with karyotype 47,XXY presented with bilateral central neurosensory retinal detachment

  3. Psychological Detachment Mediating the Daily Relationship between Workload and Marital Satisfaction

    Science.gov (United States)

    Germeys, Lynn; De Gieter, Sara

    2017-01-01

    Scholars already demonstrated that psychologically detaching from work after workhours can diminish or avoid the negative effects of job demands on employees' well-being. In this study, we examined a curvilinear relationship between workload and psychological detachment. Moreover, we investigated the moderating influence of an employee's work-home segmentation preference on the relation between detachment and marital satisfaction. In addition, we applied and extended the stressor-detachment model by examining detachment as a mediator of the relation between workload and marital satisfaction. A total of 136 employees participated in our daily diary survey study during 10 consecutive working days. The results of the Bayesian 2-level path analyses revealed a negative linear and curvilinear relationship between workload and psychological detachment on a daily basis. Daily detachment positively related to marital satisfaction, with one's preference to segment work from home reinforcing this relationship. Moreover, psychological detachment fully mediated the daily relationship between workload and marital satisfaction. Implications for practice and suggestions for future research are discussed. PMID:28101076

  4. Fasudil, a Clinically Used ROCK Inhibitor, Stabilizes Rod Photoreceptor Synapses after Retinal Detachment.

    Science.gov (United States)

    Townes-Anderson, Ellen; Wang, Jianfeng; Halász, Éva; Sugino, Ilene; Pitler, Amy; Whitehead, Ian; Zarbin, Marco

    2017-06-01

    Retinal detachment disrupts the rod-bipolar synapse in the outer plexiform layer by retraction of rod axons. We showed that breakage is due to RhoA activation whereas inhibition of Rho kinase (ROCK), using Y27632, reduces synaptic damage. We test whether the ROCK inhibitor fasudil, used for other clinical applications, can prevent synaptic injury after detachment. Detachments were made in pigs by subretinal injection of balanced salt solution (BSS) or fasudil (1, 10 mM). In some animals, fasudil was injected intravitreally after BSS-induced detachment. After 2 to 4 hours, retinae were fixed for immunocytochemistry and confocal microscopy. Axon retraction was quantified by imaging synaptic vesicle label in the outer nuclear layer. Apoptosis was analyzed using propidium iodide staining. For biochemical analysis by Western blotting, retinal explants, detached from retinal pigmented epithelium, were cultured for 2 hours. Subretinal injection of fasudil (10 mM) reduced retraction of rod spherules by 51.3% compared to control detachments (n = 3 pigs, P = 0.002). Intravitreal injection of 10 mM fasudil, a more clinically feasible route of administration, also reduced retraction (28.7%, n = 5, P ROCK, was decreased with 30 μM fasudil (n = 8-10 explants, P ROCK signaling with fasudil reduced photoreceptor degeneration and preserved the rod-bipolar synapse after retinal detachment. These results support the possibility, previously tested with Y27632, that ROCK inhibition may attenuate synaptic damage in iatrogenic detachments.

  5. Staying Well and Engaged When Demands Are High: The Role of Psychological Detachment

    Science.gov (United States)

    Sonnentag, Sabine; Binnewies, Carmen; Mojza, Eva J.

    2010-01-01

    The authors of this study examined the relation between job demands and psychological detachment from work during off-job time (i.e., mentally switching off) with psychological well-being and work engagement. They hypothesized that high job demands and low levels of psychological detachment predict poor well-being and low work engagement. They…

  6. Prevention of retinal detachment in Stickler syndrome: the Cambridge prophylactic cryotherapy protocol.

    Science.gov (United States)

    Fincham, Gregory S; Pasea, Laura; Carroll, Christopher; McNinch, Annie M; Poulson, Arabella V; Richards, Allan J; Scott, John D; Snead, Martin P

    2014-08-01

    The Stickler syndromes are the most common causes of inherited and childhood retinal detachment; however, no consensus exists regarding the effectiveness of prophylactic intervention. We evaluate the long-term safety and efficacy of the Cambridge prophylactic cryotherapy protocol, a standardized retinal prophylactic treatment developed to prevent retinal detachment arising from giant retinal tears in type 1 Stickler syndrome. Retrospective comparative case series. Four hundred eighty seven patients with type 1 Stickler syndrome. Time to retinal detachment was compared between patients who received bilateral prophylaxis and untreated controls, with and without individual patient matching. Patients receiving unilateral prophylaxis (after fellow eye retinal detachment) were similarly compared with an appropriate control subgroup. Individual patient matching ensured equal age and follow-up between groups and that an appropriate control (who had not suffered a retinal detachment before the age at which their individually matched treatment patient underwent prophylactic treatment) was selected. Matching was blinded to outcome events. Individual patient matching protocols purposely weighted bias against the effectiveness of treatment. All treatment side effects are reported. Time to retinal detachment and side effects occurring after prophylactic treatment. The bilateral control group (n = 194) had a 7.4-fold increased risk of retinal detachment compared to the bilateral prophylaxis group (n = 229) (hazard ratio [HR], 7.40; 95% confidence interval [CI], 4.53-12.08; PCambridge prophylactic cryotherapy protocol is safe and markedly reduces the risk of retinal detachment. Copyright © 2014 American Academy of Ophthalmology. Published by Elsevier Inc. All rights reserved.

  7. Postoperative recovery of visual function after macula-off rhegmatogenous retinal detachment

    NARCIS (Netherlands)

    van de Put, Mathijs A. J.; Croonen, Danna; Nolte, Ilja M.; Japing, Wouter J.; Hooymans, Johanna M. M.; Los, Leonoor I.

    2014-01-01

    Purpose: To determine which factors affect the recovery of visual function in macula-off rhegmatogenous retinal detachment (RRD). Methods: In a prospective study of forty-five patients with a primary macula-off RRD of 24 hours to 6 weeks duration, the height of the macular detachment was determined

  8. Protein changes in the retina following experimental retinal detachment in rabbits

    DEFF Research Database (Denmark)

    Mandal, Nakul; Lewis, Geoffrey P; Fisher, Steven K

    2011-01-01

    Retinal detachment leads to the widespread cellular remodeling of the retina. The purpose of this study was to identify protein changes that accompany these cellular alterations by comparing the proteomic profiles of sham and experimentally detached rabbit retina. Elucidation of the proteins most...

  9. Visual and electronic comparisons of detached strawberry leaves inoculated with two Colletotrichum species

    Science.gov (United States)

    Inoculation of detached strawberry leaves with Colletotrichum species may provide an accurate, rapid, non-destructive method of identifying anthracnose resistant germplasm. The purpose of this study was to compare two methods for screening anthracnose resistance. Detached leaves of 60 susceptible an...

  10. Evolution of radiation profiles during detached plasmas and radiative collapse in LHD

    Energy Technology Data Exchange (ETDEWEB)

    Pandya, Shwetang N., E-mail: pandya.shwetang@LHD.nifs.ac.jp [The Graduate University for Advance Studies, 322-6 Oroshi-cho, Toki 509-5292 (Japan); Peterson, Byron J.; Kobayashi, Masahiro; Mukai, Kiyofumi [National Institute for Fusion Science, 322-6 Oroshi-cho, Toki 509-5292 (Japan); Sano, Ryuichi [The Graduate University for Advance Studies, 322-6 Oroshi-cho, Toki 509-5292 (Japan); Tanaka, Hirohiko; Masuzaki, Suguru; Miyazawa, Junichi; Akiyama, Tsuyoshi; Motojima, Gen [National Institute for Fusion Science, 322-6 Oroshi-cho, Toki 509-5292 (Japan); Ohno, Noriyasu [Nagoya University, Chikusa-ku, Nagoya 464-8603 (Japan)

    2015-08-15

    The divertor heat loads can be efficiently controlled by plasma detachment, hence it is foreseen as a suitable operational regime for divertor operation of future machines. Detachment regime is normally approached by raising the plasma density to a point where plasma detaches from the divertor. If such an approach is adapted to detach the Large Helical Device (LHD) plasma, the plasma goes to radiative collapse due to thermal instabilities. Another approach to detach LHD plasma is by intrinsic impurity seeding using Ne. The discharges detach but ultimately collapse at comparatively lower densities. The detachment is achieved and sustained at high densities by the induction of an m/n = 1/1 resonant magnetic perturbation (RMP) in the stochastic edge of the LHD plasma. First quantitative measurements of the two-dimensional (2D) impurity radiation profiles from the three-dimensional (3D) plasma edge of LHD showing the time evolution of radiative collapse and RMP assisted detachment measured by recently upgraded and calibrated Infrared imaging Video Bolometer (IRVB) are presented in this article.

  11. Pregnancy Complications: Bacterial Vaginosis

    Science.gov (United States)

    ... Complications & Loss > Pregnancy complications > Bacterial vaginosis and pregnancy Bacterial vaginosis and pregnancy E-mail to a friend Please ... this page It's been added to your dashboard . Bacterial vaginosis (also called BV or vaginitis) is an infection ...

  12. Pregnancy Complications: Bacterial Vaginosis

    Science.gov (United States)

    ... Loss > Pregnancy complications > Bacterial vaginosis and pregnancy Bacterial vaginosis and pregnancy E-mail to a friend Please ... page It's been added to your dashboard . Bacterial vaginosis (also called BV or vaginitis) is an infection ...

  13. Bacterial vaginosis -- aftercare

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/patientinstructions/000687.htm Bacterial vaginosis - aftercare To use the sharing features on this ... back after you use the bathroom. Preventing Bacterial Vaginosis You can help prevent bacterial vaginosis by: Not ...

  14. Bacterial pericarditis (image)

    Science.gov (United States)

    Bacterial pericarditis is an inflammation of the pericardium, the sac-like covering of the heart, caused by a bacterial infection. The bacterial infection causes inflammation and swelling of the pericardium. Pain ...

  15. Rovibrationally Resolved Time-Dependent Collisional-Radiative Model of Molecular Hydrogen and Its Application to a Fusion Detached Plasma

    Directory of Open Access Journals (Sweden)

    Keiji Sawada

    2016-12-01

    Full Text Available A novel rovibrationally resolved collisional-radiative model of molecular hydrogen that includes 4,133 rovibrational levels for electronic states whose united atom principal quantum number is below six is developed. The rovibrational X 1 Σ g + population distribution in a SlimCS fusion demo detached divertor plasma is investigated by solving the model time dependently with an initial 300 K Boltzmann distribution. The effective reaction rate coefficients of molecular assisted recombination and of other processes in which atomic hydrogen is produced are calculated using the obtained time-dependent population distribution.

  16. Kinetic effects in edge plasma: kinetic modeling for edge plasma and detached divertor

    Science.gov (United States)

    Takizuka, T.

    2017-03-01

    Detached divertor is considered a solution for the heat control in magnetic-confinement fusion reactors. Numerical simulations using the comprehensive divertor codes based on the plasma fluid modeling are indispensable for the design of the detached divertor in future reactors. Since the agreement in the results between detached-divertor experiments and simulations has been rather fair but not satisfactory, further improvement of the modeling is required. The kinetic effect is one of key issues for improving the modeling. Complete kinetic behaviors are able to be simulated by the kinetic modeling. In this paper at first, major kinetic effects in edge plasma and detached divertor are listed. One of the most powerful kinetic models, particle-in-cell (PIC) model, is described in detail. Several results of PIC simulations of edge-plasma kinetic natures are presented. Future works on PIC modeling and simulation for the deeper understanding of edge plasma and detached divertor are discussed.

  17. Neglected bacterial zoonoses.

    Science.gov (United States)

    Chikeka, I; Dumler, J S

    2015-05-01

    Bacterial zoonoses comprise a group of diseases in humans or animals acquired by direct contact with or by oral consumption of contaminated animal materials, or via arthropod vectors. Among neglected infections, bacterial zoonoses are among the most neglected given emerging data on incidence and prevalence as causes of acute febrile illness, even in areas where recognized neglected tropical diseases occur frequently. Although many other bacterial infections could also be considered in this neglected category, five distinct infections stand out because they are globally distributed, are acute febrile diseases, have high rates of morbidity and case fatality, and are reported as commonly as malaria, typhoid or dengue virus infections in carefully designed studies in which broad-spectrum diagnoses are actively sought. This review will focus attention on leptospirosis, relapsing fever borreliosis and rickettsioses, including scrub typhus, murine typhus and spotted fever group rickettsiosis. Of greatest interest is the lack of distinguishing clinical features among these infections when in humans, which confounds diagnosis where laboratory confirmation is lacking, and in regions where clinical diagnosis is often attributed to one of several perceived more common threats. As diseases such as malaria come under improved control, the real impact of these common and under-recognized infections will become evident, as will the requirement for the strategies and allocation of resources for their control.

  18. Outcomes of Surgical Treatment for Retinal Detachment Resulting from Macular Hole in Highly Myopic Eyes

    Directory of Open Access Journals (Sweden)

    Amir-Khosro Ghaseminejad

    2008-12-01

    Full Text Available

    PURPOSE: To determine the type and outcome of surgery for retinal detachment resulting from macular hole in highly myopic eyes. METHODS: This retrospective analysis was performed on the medical records of highly myopic patients who underwent surgery for retinal detachment (RD resulting from macular hole at Labbafinejad Hospital, Tehran-Iran from 1992 to 2001. Variables included age, gender, number and type of operations, visual acuity before and after the procedures and surgical success rate. RESULTS: Overall, 28 eyes of 27 patients (26 female and one male with mean age of 59.8±11 years were included. Mean follow-up was 17.3 (range 3-72 months. Mean axial length was 29±2.74mm (range: 24 to 35mm and mean degree of myopia was -16.4±3.1 D (range -10 to -22 D. Posterior staphyloma was present in 20 eyes (71%. Seven eyes had undergone failed scleral buckling as the primary procedure prior to referral. Intravitreal SF6 injection was the primary procedure in 12 eyes with localized detachments; the retina became attached in 5 (41.6% of these eyes, however redetachment occurred in 7 (58.4% eyes. Overall, 23 eyes (including 7 failed scleral buckling cases, 7 redetachments

  19. Effect of Symptom Duration until Surgery on First-Year Outcome in Pseudophakic Primary Rhegmatogenous Retinal Detachment.

    Science.gov (United States)

    Enders, Philip; Schick, Tina; Kemper, Carolin; Schaub, Friederike; Fauser, Sascha

    2017-01-01

    To investigate the effect of symptom duration in pseudophakic primary rhegmatogenous retinal detachment (RRD) with or without macular involvement on functional and anatomical outcome during first-year follow-up. Retrospective review of cases with pars plana vitrectomy for retinal detachment repair. Symptom duration, functional outcome, and redetachment rates were analyzed. Symptom duration was grouped (1-3, 4-7, 8-14, 15-30, >30 days) and correlated to outcome in the first year of follow-up. 371 macula-off and 230 macula-on RRDs could be included. In macula-off RRDs, longer symptom duration was associated with higher redetachment rates (10-21%; p = 0.032). Patients presenting symptoms for less than 8 days showed significantly lower rates than those with a longer symptom duration (p = 0.046). In macula-on RRDs, symptom duration and outcome were not associated (p > 0.6). In macula-off RRD, early reattachment surgery is necessary to lower the risk for retinal redetachment. The aim in macula-on RRD is to prevent conversion to a macula-off situation. © 2017 S. Karger AG, Basel.

  20. A retrospective study comparing outcomes of primary rhegmatogenous retinal detachment repair by scleral buckling and pars plana vitrectomy in Finland

    Directory of Open Access Journals (Sweden)

    Sahanne S

    2017-03-01

    Full Text Available Sari Sahanne,1 Raimo Tuuminen,2 Jari Haukka,3 Sirpa Loukovaara4 1Department of Anesthesiology, Helsinki University Central Hospital, Helsinki, 2Department of Ophthalmology, Kymenlaakso Central Hospital, Kotka, 3Hjelt Institute, Faculty of Medicine, University of Helsinki, 4Unit of Vitreoretinal Surgery, Department of Ophthalmology, Helsinki University Central Hospital, University of Helsinki, Helsinki, Finland Background: Rhegmatogenous retinal detachment (RRD is the most common form of retinal detachment and an ophthalmic emergency. Here, we compared outcomes of primary RRD eyes operated with conventional scleral buckling (SB with cryoretinopexy to those operated with standard pars plana vitrectomy (PPV. Methods: This is an institutional, retrospective, register-based, observational, comparative study. Based on the surgical procedure, 319 eyes of 319 patients were divided into two groups: SB plus cryotherapy (n=50 and PPV (n=269. Changes in intraocular pressure (IOP and best-corrected visual acuity (BCVA were recorded at 30 days and reoperation rates within 180 days postoperatively. Results: Eyes operated with PPV had less reoperations within the first 180 days as compared with SB eyes (P=0.001, log-rank test; however, changes in IOP were more prominent (mean ± standard deviation: +8.1±8.8 vs. +4.4±7.0 mmHg, respectively; P=0.006. Changes in BCVA did not differ between the surgical procedures. Conclusion: PPV was associated with higher primary anatomic success rates and lower risk of reoperation but significant IOP elevation when compared to SB. These factors should be case-specifically considered when choosing treatment modality for primary RRD. Keywords: rhegmatogenous retinal detachment, vitrectomy, scleral buckling

  1. The Western Cycladic Detachment System on Makronisos, Greece

    Science.gov (United States)

    Loisl, Johannes; Lindner, Karoline; Huet, Benjamin; Grasemann, Bernhard; Rice, A. Hugh. N.; Soukis, Konstantinos; Schneider, David

    2014-05-01

    Makronisos, which lies 3 km east of the Attica port of Lavrion, is the northwesternmost part of the Western Cycladic archipelago. The Cyclades and adjacent part of Attica are dominated by Miocene low-angle detachments that developed during top-to-SSW crustal extension, forming the West Cycladic Detachment System. Although extension is well documented on the other Western Cycladic islands and in Attica, the geology of Makronisos is poorly known. The aim of this study is to provide data on the structural, microstructural and metamorphic evolution of Makronisos to resolve its tectonostratigraphic position and its relationships within the Cycladic realm. Most of Makronisos consists of grey, locally graphitic, pelitic schists and yellowish impure marbles, interlayed with blue-grey mylonitic marbles and quartzites, forming large-scale pinch-and-swell structures. Metabasites are present as small bodies along the east side of the island but are thicker and more continuous in the southeast. Petrography shows that metabasites usually contain blue amphiboles, although generally only as relicts after greenschist facies retrogression. Serpentinite has been found at two localities. The structurally highest level of the island consists of white-grey to pale-red ultramylonites up to 40 m thick. These mainly lie on the central ridge of the island, but, due to large-scale upright folding, also crop out along the east and west coasts. In several places, the ultramylonites overlie 1-2 m of foliated ultracataclasites derived from the footwall pelitic schists. Stretching lineations and macroscopic shear-criteria indicate a top-to-SSW shear-sense. Microstructural analyses consistently show the same shear-sense, indicated by shape and crystal preferred orientations, σ- and δ-clasts, mica-fish, rotated veins and SCC' structures. Deformation mechanisms observed in quartz (LT-bulging) and calcite (recrystallization) are evidence for deformation temperatures of c. 300°C. Albite

  2. Interaction between resource identity and bacterial community composition regulates bacterial respiration in aquatic ecosystems

    Directory of Open Access Journals (Sweden)

    A. P. F. Pires

    Full Text Available Abstract Resource identity and composition structure bacterial community, which in turn determines the magnitude of bacterial processes and ecological services. However, the complex interaction between resource identity and bacterial community composition (BCC has been poorly understood so far. Using aquatic microcosms, we tested whether and how resource identity interacts with BCC in regulating bacterial respiration and bacterial functional diversity. Different aquatic macrophyte leachates were used as different carbon resources while BCC was manipulated through successional changes of bacterial populations in batch cultures. We observed that the same BCC treatment respired differently on each carbon resource; these resources also supported different amounts of bacterial functional diversity. There was no clear linear pattern of bacterial respiration in relation to time succession of bacterial communities in all leachates, i.e. differences on bacterial respiration between different BCC were rather idiosyncratic. Resource identity regulated the magnitude of respiration of each BCC, e.g. Ultricularia foliosa leachate sustained the greatest bacterial functional diversity and lowest rates of bacterial respiration in all BCC. We conclude that both resource identity and the BCC interact affecting the pattern and the magnitude of bacterial respiration in aquatic ecosystems.

  3. Rhegmatogenous retinal detachment: a reappraisal of its pathophysiology and treatment.

    Science.gov (United States)

    Kuhn, Ferenc; Aylward, Bill

    2014-01-01

    This article represents a synthesis of an extensive literature review and the authors' decades-long personal experience with both scleral buckling (SB) and vitrectomy for rhegmatogenous retinal detachment (RD). Presenting a coherent understanding of the pathophysiology and treatment of RD, the authors confirm numerous findings described in earlier publications but also challenge certain long-standing dogmas. The key argument made here is that it is extremely rare for the chain of events leading to an RD to start with a retinal pathology. Rather, the initial pathology is syneresis of the vitreous, which then allows gel movement (intraocular currents). At the point of vitreoretinal adhesion, dynamic traction is exerted on the retina, which may be sufficient to tear it. If the tear is operculated and the dynamic traction overcomes the forces keeping the neuroretina and retinal pigment epithelium (RPE) together, the heretofore virtual subretinal space becomes accessible through the retinal tear. The intraocular currents allow the free (nonbound) intravitreal fluid to enter the subretinal space, and once the amount of the incoming fluid overwhelms the draining capacity of the RPE, an RD ensues. Detachment of the posterior cortical vitreous (PVD) is not a necessary prerequisite to RD development; furthermore, PVD cannot be diagnosed preoperatively with adequate certainty with current technology such as biomicroscopy, ultrasonography or optical coherence tomography. The surgeon should expect no or only partial (anomalous) PVD at the time of surgical repair in over half of eyes. The treatment's primary goal must thus be weakening (pneumatic retinopexy, SB) or eliminating (vitrectomy) this dynamic traction. If vitrectomy is employed, it must be a truly complete vitreous removal. This includes a surgically induced PVD if one is not present, close shaving at the periphery, and removing the vitreous immediately behind the lens. The vitrectomy is followed by the creation of a

  4. 大理某医院检验报告单的带菌情况调查及消毒方法研究%Survey on the Rates and Species of Bacterial Contamination of Laboratory Sheets and Disinfection Methods

    Institute of Scientific and Technical Information of China (English)

    董立文; 王菲; 沈彩玲; 张秀丹; 陆绍花; 王涛

    2011-01-01

    目的:对医院检验报告单的带菌情况进行调查及消毒方法研究。方法:先直接涂抹检验报告单取样培养。检验报告单采用紫外线消毒法、甲醛熏蒸消毒法、戊二醛熏蒸消毒法、微波消毒法消毒后,再涂抹检验报告单取样后接种培养。结果:紫外线消毒法,消毒前共生长菌292株,消毒后共长菌146株,消毒灭菌率为50%;甲醛熏蒸消毒法,消毒前共生长菌121株,消毒后共长菌76株,消毒灭菌率为37.19%;戊二醛熏蒸消毒法,未消毒共生长菌125株,消毒后共长菌60株,消毒灭菌率为52%;微波消毒法,消毒前共生长菌550株,消毒后共长菌189株,消毒灭菌率为65.64%。结论:微波消毒法是消毒检验报告单的较好方法。%Objective: To investigate the rates of bacterial contamination of laboratory sheets and relevant disinfection methods.Method: Laboratory sheets were sampled directly and bacteria were cultured in medium,and then laboratory sheets were disinfected by using different sterile techniques including ultraviolet sterilization,formaldehyde fumigation sterilization,glutaric dialdehyde fumigation method and microwave.Finally,those laboratory sheets were sampled directly again and bacteria were cultured again.Results: Two hundred and ninety-two strains bacteria grew on culture medium before ultraviolet sterilization and 146 strains after;the sterilization rate was 50%.There were 121 strains bacteria before formaldehyde fumigation sterilization and 76 strains after;the sterilization rate was 37.19%.There were 125 strains before glutaric dialdehyde fumigation sterilization and 60 strains after;the sterilization rate was 52%.There were 550 strains bacteria before Microwave sterilization and 189 strains after;the sterilization rate was 65.64%.Conclusion: Microwave sterilization showed better sterilization rate in laboratory sheets sterilization.

  5. Risk of pseudophakic retinal detachment in 202 226 patients using the fellow nonoperated eye as reference

    DEFF Research Database (Denmark)

    Bjerrum, Søren S; Mikkelsen, Kim Lyngby; La Cour, Morten

    2013-01-01

    To study the risk of pseudophakic retinal detachment (PRD) after first-eye phacoemulsification cataract surgery in Denmark relative to the risk of retinal detachment (RD) in the patients' fellow nonoperated eyes.......To study the risk of pseudophakic retinal detachment (PRD) after first-eye phacoemulsification cataract surgery in Denmark relative to the risk of retinal detachment (RD) in the patients' fellow nonoperated eyes....

  6. Strain localisation in mechanically layered rocks beneath detachment zones: insights from numerical modelling

    Directory of Open Access Journals (Sweden)

    L. Le Pourhiet

    2013-04-01

    Full Text Available We have designed a series of fully dynamic numerical simulations aimed at assessing how the orientation of mechanical layering in rocks controls the orientation of shear bands and the depth of penetration of strain in the footwall of detachment zones. Two parametric studies are presented. In the first one, the influence of stratification orientation on the occurrence and mode of strain localisation is tested by varying initial dip of inherited layering in the footwall with regard to the orientation of simple shear applied at the rigid boundary simulating a rigid hanging wall, all scaling and rheological parameter kept constant. It appears that when Mohr–Coulomb plasticity is being used, shear bands are found to localise only when the layering is being stretched. This corresponds to early deformational stages for inital layering dipping in the same direction as the shear is applied, and to later stages for intial layering dipping towards the opposite direction of shear. In all the cases, localisation of the strain after only γ=1 requires plastic yielding to be activated in the strong layer. The second parametric study shows that results are length-scale independent and that orientation of shear bands is not sensitive to the viscosity contrast or the strain rate. However, decreasing or increasing strain rate is shown to reduce the capacity of the shear zone to localise strain. In the later case, the strain pattern resembles a mylonitic band but the rheology is shown to be effectively linear. Based on the results, a conceptual model for strain localisation under detachment faults is presented. In the early stages, strain localisation occurs at slow rates by viscous shear instabilities but as the layered media is exhumed, the temperature drops and the strong layers start yielding plastically, forming shear bands and localising strain at the top of the shear zone. Once strain localisation has occured, the deformation in the shear band becomes

  7. A New Microsurgical Technique to Correct Retinal Detachment

    Institute of Scientific and Technical Information of China (English)

    Wen Liu; Chunfang Li; Suying Huang; Qichong Wu

    2006-01-01

    Purpose: To evaluate the efficacy of a new technique to repair retinal detachments (RD) under the microscope. Methods: Thirty-six consecutive patients (36 eyes) who presented to our clinic with rhegmatogenous RD without severe proliferative vitreoretinopathy (≤ C1) were included. The sutures for buckling and/or encircling bands were preplaced according to the preoperative location of the breaks using a three-mirror contact lens. Drainage of subretinal fluid, retinal cryotherapy, buckling, locating the retinal breaks, and intravitreal gases injection were performed under surgical microscopy. The surgical effects were compared with those in 37 consecutive patients with rhegmatogenous RD who underwent surgery under binocular indirect ophthalmoscopy.Results: The simultaneous intraoperative observation of fundus details and the sclera through the microscope was excellent in all cases. The effect of retinal cryotherapy was clearly visible. Mild opacity of the refractive media did not interfere with observing cryotherapy and locating the breaks. Retinal reattachment was obtained in31 eyes (86%) during the primary surgery and in three eyes after a second surgery(94% total). The best-corrected visual acuity was <0.1 in 6 eyes (16.7%), 0.1~0.4 in 15 eyes (41.7%) and ≥ 0.5 in 15 eyes (41.7%). The results were similar to that of RD surgery performed under indirect ophthalmoscopy.Conclusions: This microsurgical procedure to correct RD is simple, convenient,reliable, provides an upright image, and facilitates good recovery similar to conventional RD surgery.

  8. A new detached K7 dwarf eclipsing binary system

    CERN Document Server

    Young, T B; Webb, J K; Ashley, M C B; Christiansen, J L; Derekas, A; Nutto, C

    2006-01-01

    We present an analysis of a new, detached, double-lined eclipsing binary system with K7 Ve components, discovered as part of the University of New South Wales Extrasolar Planet Search. The object is significant in that only 6 other binary systems are known with comparable or lower mass. Such systems offer important tests of mass-radius theoretical models. Follow-up photometry and spectroscopy were obtained with the 40-inch and 2.3m telescopes at SSO respectively. An estimate of the radial velocity amplitude from spectral absorption features, combined with the orbital inclination (83.5 deg) estimated from lightcurve fitting, yielded a total mass of M=(1.041 +/- 0.06)M_sun and component masses of M_A=(0.529 +/- 0.035)M_sun and M_B=(0.512 +/- 0.035)M_sun. The radial velocity amplitude estimated from absorption features (167 +/- 3)kmps was found to be less than the estimate from the H_alpha emission lines (175 +/- 1.5)kmps. The lightcurve fit produced radii of R_A=(0.641 +/- 0.05)R_sun and R_B=(0.608 +/- 0.06)R_s...

  9. Molecular mechanisms of photochemically induced posterior vitreous detachment.

    Science.gov (United States)

    Kakehashi, A; Ueno, N; Chakrabarti, B

    1994-01-01

    Vitreous gel contraction and syneresis, commonly associated with age- and disease-related posterior vitreous detachment (PVD), were induced by a hematoporphyrin (HP)-photosensitized reaction. Calf vitreous gel was irradiated by white light in the presence of HP. Gel weights of the vitreous samples after 24 h of irradiation decreased by 14%, the irradiated control without HP by 8% and the control with HP stored in the dark by 8%. No significant difference in vitreous gel compressibility was found between the irradiated controls and the irradiated samples. In separate experiments, collagen gel in a glass capillary and hyaluronic acid (HA) were irradiated with white light in the presence of HP. The control collagen gel (irradiated without HP and stored in the dark with HP) decreased in length by 0.6% after 96 h, the experimental gel with HP decreased in length by 1.3 and 1.9% after 24- and 96-hour irradiation by visible light, respectively. The irradiated HA monitored by high-performance liquid chromatography showed a molecular weight decrease in the HP-treated polymer. Because the HP-sensitized reaction predominantly produces singlet oxygen, collagen gel contraction and HA degradation, in this case, are likely caused by this active oxygen species.

  10. Rocket dust storms and detached layers in the Martian atmosphere

    CERN Document Server

    Spiga, Aymeric; Madeleine, Jean-Baptiste; Määttänen, Anni; Forget, François

    2012-01-01

    Airborne dust is the main climatic agent in the Martian environment. Local dust storms play a key role in the dust cycle; yet their life cycle is poorly known. Here we use mesoscale modeling with radiatively-active transported dust to predict the evolution of a local dust storm monitored by OMEGA on board Mars Express. We show that the evolution of this dust storm is governed by deep convective motions. The supply of convective energy is provided by the absorption of incoming sunlight by dust particles, in lieu of latent heating in moist convection on Earth. We propose to use the terminology "rocket dust storm", or conio-cumulonimbus, to describe those storms in which rapid and efficient vertical transport takes place, injecting dust particles at high altitudes in the Martian troposphere (30 to 50 km). Combined to horizontal transport by large-scale winds, rocket dust storms form detached layers of dust reminiscent of those observed with instruments on board Mars Global Surveyor and Mars Reconnaissance Orbite...

  11. [Vitreo-retinal surgery for complicated retinal detachment].

    Science.gov (United States)

    Wang, J Z

    1993-07-01

    93 eyes (93 patients) of complicated retinal detachment were treated with vitreo-retinal surgery. Among the series, 75 eyes were rhegmatogenous with PVR C3-D3 in 66 eyes (88.0%), while the remaining 18 eyes were traction induced. None of the cases had giant tears or complicating diabetes. On discharge from the hospital, the operation was effective in 62 cases (66.7%), in whom the retina was totally reattached or only a small amount of subretinal fluid remained. In a group of 40 eyes where the inert gas SF6 was used, the operation was effective in 30 cases (75.0%). 41 cases were followed up postoperatively for over 3 months, averaging 13.7 months, to find the operative results stable in 33 eyes (80.5%), with the visual acuity improved in 22 cases (66.7%), unchanged in 9 cases (27.3%), and decreased in 2 cases (6.0%). The operative procedures, the peeling of pre-retinal membrane, the effect of PVR severity on the operative results, and the promotion of operative efficacy by application of wide encircling buckle and inert gas tamponade were discussed.

  12. A Mathematical Model of Quorum Sensing Induced Biofilm Detachment.

    Science.gov (United States)

    Emerenini, Blessing O; Hense, Burkhard A; Kuttler, Christina; Eberl, Hermann J

    2015-01-01

    Cell dispersal (or detachment) is part of the developmental cycle of microbial biofilms. It can be externally or internally induced, and manifests itself in discrete sloughing events, whereby many cells disperse in an instance, or in continuous slower dispersal of single cells. One suggested trigger of cell dispersal is quorum sensing, a cell-cell communication mechanism used to coordinate gene expression and behavior in groups based on population densities. To better understand the interplay of colony growth and cell dispersal, we develop a dynamic, spatially extended mathematical model that includes biofilm growth, production of quorum sensing molecules, cell dispersal triggered by quorum sensing molecules, and re-attachment of cells. This is a highly nonlinear system of diffusion-reaction equations that we study in computer simulations. Our results show that quorum sensing induced cell dispersal can be an efficient mechanism for bacteria to control the size of a biofilm colony, and at the same time enhance its downstream colonization potential. In fact we find that over the lifetime of a biofilm colony the majority of cells produced are lost into the aqueous phase, supporting the notion of biofilms as cell nurseries. We find that a single quorum sensing based mechanism can explain both, discrete dispersal events and continuous shedding of cells from a colony. Moreover, quorum sensing induced cell dispersal affects the structure and architecture of the biofilm, for example it might lead to the formation of hollow inner regions in a biofilm colony.

  13. Vertical detachment energies of anionic thymidine: Microhydration effects

    Science.gov (United States)

    Kim, Sunghwan; Schaefer, Henry F.

    2010-10-01

    Density functional theory has been employed to investigate microhydration effects on the vertical detachment energy (VDE) of the thymidine anion by considering the various structures of its monohydrates. Structures were located using a random searching procedure. Among 14 distinct structures of the anionic thymidine monohydrate, the low-energy structures, in general, have the water molecule bound to the thymine base unit. The negative charge developed on the thymine moiety increases the strength of the intermolecular hydrogen bonding between the water and base units. The computed VDE values of the thymidine monohydrate anions are predicted to range from 0.67 to 1.60 eV and the lowest-energy structure has a VDE of 1.32 eV. The VDEs of the monohydrates of the thymidine anion, where the N1H hydrogen of thymine has been replaced by a 2'-deoxyribose ring, are greater by ˜0.30 eV, compared to those of the monohydrates of the thymine anion. The results of the present study are in excellent agreement with the accompanying experimental results of Bowen and co-workers [J. Chem. Phys. 133, 144304 (2010)].

  14. Documents and detachment in the figurational sociology of sport

    Directory of Open Access Journals (Sweden)

    Paddy Dolan

    2015-01-01

    Full Text Available This article discusses the methodological implications of using a figurational approach to the study of Gaelic games (the Irish sports of hurling and Gaelic football and their organisation and governance from the late nineteenth century onwards. Following Norbert Elias, we argue that data generation guided by theories, and potentially modifying such theories, are essential components of research endeavours. The problem of generalisation or representativeness of historical documents can be addressed by treating documents as part of a fi guration of evidence, thereby constituting a mutually supporting network of data that serves as a scaffold or structure for theoretical and empirical findings. Based on the experience of using documents such as newspaper and referee reports of matches, and also organisational documents such as the minutes of meetings at various levels of governance, we contribute to the discussion concerning borders and boundary maintenance between history, sociology and historical sociology. While we elaborate on the Eliasian perspective on emotional detachment, and secondary involvement, when conducting research, we also note the dificulties in avoiding ‘preferred’ readings of national history given that Gaelic games have been intertwined with the narrative and project of national self-determination in Ireland. Researchers are compelled to recognise the theoretical significance of sports as a motor and symbol of emotional identifications, but must also create distance from their own national habitus in order to prioritise participation in intergenerational knowledge generation over further national myth-making.

  15. Clinical and multidetector CT follow-up results of renal artery aneurysms treated by detachable coil embolization using 3D rotational angiography

    Energy Technology Data Exchange (ETDEWEB)

    Seo, Jung Min; Park, Kwang Bo; Kim, Keon Ha; Jeon, Pyoung; Shin, Sung Wook; Park, Hong Suk; Do, Young Soo (Dept. of Radiology and the Center for Imaging Science, Samsung Medical Center, Sungkyunkwan Univ. School of Medicine, Seoul (Korea, Republic of)), email: kbparksmc@skku.edu; Kim, Dong-Ik; Kim, Young-Wook (Div. of Vascular Surgery, Dept. of Surgery, Samsung Medical Center, Sungkyunkwan Univ. School of Medicine, Seoul (Korea, Republic of))

    2011-10-15

    Background: There are very few reports regarding the use of 3D rotational angiography (3D RA) in embolization of renal artery aneurysms (RAAs). No valuable data have been reported on the follow-up result of coil embolization for RAAs on computed tomography (CT). Purpose: To evaluate the clinical and multidetector computed tomography (MDCT) follow-up results of renal artery aneurysms treated by detachable coil embolization using 3D RA. Material and Methods: Six patients diagnosed with RAAs were included. Five patients underwent detachable coil embolization. Five patients underwent 3D RA and the parameters used for planning endovascular treatment obtained by 2D CT, reformatted 3D CT angiography (3D CTA), 2D digital subtraction angiography (2D DSA) and 3D RA were compared. The post embolization MDCT follow-up findings were analyzed retrospectively. Results: The technical success rate for detachable coil embolization was 40%. The 3D CTA showed the dome-to-neck ratio (DNR) and tangential view of the renal artery aneurysm in five patients (83.3%) and the 2D CT showed it in four (66.7%). An optimal working angle assessment could not be obtained on the 2D CT and 3D CTA. The 3D RA showed the DNR, tangential view, and optimal working angle in all patients. Renal infarction occurred in three patients and Postprocedural hypertension developed in two patients during the follow-up period. Conclusion: The 3D RA was useful in preoperative determination of adequate working angle for detachable coil embolization of RAAs. Late complications of detachable coil embolization for RAAs were renal infarction and hypertension

  16. A Meta-Analysis on Antecedents and Outcomes of Detachment from Work.

    Science.gov (United States)

    Wendsche, Johannes; Lohmann-Haislah, Andrea

    2016-01-01

    Detachment from work has been proposed as an important non-work experience helping employees to recover from work demands. This meta-analysis (86 publications, k = 91 independent study samples, N = 38,124 employees) examined core antecedents and outcomes of detachment in employee samples. With regard to outcomes, results indicated average positive correlations between detachment and self-reported mental (i.e., less exhaustion, higher life satisfaction, more well-being, better sleep) and physical (i.e., lower physical discomfort) health, state well-being (i.e., less fatigue, higher positive affect, more intensive state of recovery), and task performance (small to medium sized effects). However, average relationships between detachment and physiological stress indicators and work motivation were not significant while associations with contextual performance and creativity were significant, but negative. Concerning work characteristics, as expected, job demands were negatively related and job resources were positively related to detachment (small sized effects). Further, analyses revealed that person characteristics such as negative affectivity/neuroticism (small sized effect) and heavy work investment (medium sized effect) were negatively related to detachment whereas detachment and demographic variables (i.e., age and gender) were not related. Moreover, we found a medium sized average negative relationship between engagement in work-related activities during non-work time and detachment. For most of the examined relationships heterogeneity of effect sizes was moderate to high. We identified study design, samples' gender distribution, and affective valence of work-related thoughts as moderators for some of these aforementioned relationships. The results of this meta-analysis point to detachment as a non-work (recovery) experience that is influenced by work-related and personal characteristics which in turn is relevant for a range of employee outcomes.

  17. A Meta-Analysis on Antecedents and Outcomes of Detachment from Work

    Science.gov (United States)

    Wendsche, Johannes; Lohmann-Haislah, Andrea

    2017-01-01

    Detachment from work has been proposed as an important non-work experience helping employees to recover from work demands. This meta-analysis (86 publications, k = 91 independent study samples, N = 38,124 employees) examined core antecedents and outcomes of detachment in employee samples. With regard to outcomes, results indicated average positive correlations between detachment and self-reported mental (i.e., less exhaustion, higher life satisfaction, more well-being, better sleep) and physical (i.e., lower physical discomfort) health, state well-being (i.e., less fatigue, higher positive affect, more intensive state of recovery), and task performance (small to medium sized effects). However, average relationships between detachment and physiological stress indicators and work motivation were not significant while associations with contextual performance and creativity were significant, but negative. Concerning work characteristics, as expected, job demands were negatively related and job resources were positively related to detachment (small sized effects). Further, analyses revealed that person characteristics such as negative affectivity/neuroticism (small sized effect) and heavy work investment (medium sized effect) were negatively related to detachment whereas detachment and demographic variables (i.e., age and gender) were not related. Moreover, we found a medium sized average negative relationship between engagement in work-related activities during non-work time and detachment. For most of the examined relationships heterogeneity of effect sizes was moderate to high. We identified study design, samples' gender distribution, and affective valence of work-related thoughts as moderators for some of these aforementioned relationships. The results of this meta-analysis point to detachment as a non-work (recovery) experience that is influenced by work-related and personal characteristics which in turn is relevant for a range of employee outcomes. PMID:28133454

  18. A retrospective study comparing outcomes of primary rhegmatogenous retinal detachment repair by scleral buckling and pars plana vitrectomy in Finland

    Science.gov (United States)

    Sahanne, Sari; Tuuminen, Raimo; Haukka, Jari; Loukovaara, Sirpa

    2017-01-01

    Background Rhegmatogenous retinal detachment (RRD) is the most common form of retinal detachment and an ophthalmic emergency. Here, we compared outcomes of primary RRD eyes operated with conventional scleral buckling (SB) with cryoretinopexy to those operated with standard pars plana vitrectomy (PPV). Methods This is an institutional, retrospective, register-based, observational, comparative study. Based on the surgical procedure, 319 eyes of 319 patients were divided into two groups: SB plus cryotherapy (n=50) and PPV (n=269). Changes in intraocular pressure (IOP) and best-corrected visual acuity (BCVA) were recorded at 30 days and reoperation rates within 180 days postoperatively. Results Eyes operated with PPV had less reoperations within the first 180 days as compared with SB eyes (P=0.001, log-rank test); however, changes in IOP were more prominent (mean ± standard deviation: +8.1±8.8 vs. +4.4±7.0 mmHg, respectively; P=0.006). Changes in BCVA did not differ between the surgical procedures. Conclusion PPV was associated with higher primary anatomic success rates and lower risk of reoperation but significant IOP elevation when compared to SB. These factors should be case-specifically considered when choosing treatment modality for primary RRD. PMID:28331284

  19. Influence of adjuvant detached mindfulness and stress management training compared to pharmacologic treatment in primiparae with postpartum depression.

    Science.gov (United States)

    Ahmadpanah, Mohammad; Nazaribadie, Marzieh; Aghaei, Elham; Ghaleiha, Ali; Bakhtiari, Azade; Haghighi, Mohammad; Bahmani, Dena Sadeghi; Akhondi, Amineh; Bajoghli, Hafez; Jahangard, Leila; Holsboer-Trachsler, Edith; Brand, Serge

    2017-07-18

    Ten to 15% of mothers experience postpartum depression (PPD). If untreated, PPD may negatively affect mothers' and infants' mental health in the long term. Accordingly, effective treatments are required. In the present study, we investigated the effect of detached mindfulness (DM) and stress management training (SMT) as adjuvants, compared to pharmacologic treatment only, on symptoms of depression in women with PPD. Forty-five primiparae (mean age: M = 24.5 years) with diagnosed PPD and treated with an SSRI (citalopram; CIT) took part in the study. At baseline, they completed questionnaires covering socio-demographic data and symptoms of depression. Experts rated also symptoms of depression. Next, participants were randomly assigned to one of the following study conditions: adjuvant detached mindfulness (CIT+DM); adjuvant stress management training (CIT+SMT); control condition (CIT). Self- and experts' ratings were completed at the end of the study 8 weeks later, and again at 8 weeks follow-up. Symptoms of depression decreased significantly over time, but more so in the CIT+DM and CIT+SMT group, compared to the control condition. The pattern of results remained stable at follow-up. In primiparae with PPD and treated with a standard SSRI, adjuvant psychotherapeutic interventions led to significant and longer-lasting improvements.

  20. On the electric breakdown field of the mesosphere and the influence of electron detachment

    DEFF Research Database (Denmark)

    Neubert, Torsten; Chanrion, Olivier Arnaud

    2013-01-01

    It has been suggested recently that electron associative detachment from negative atomic oxygen ions provides an additional source of free electrons in electric discharges of the mesosphere, the sprites, and gigantic jets. Here we study attachment under some simplifying assumptions and show...... for electron avalanches to grow. Detachment is likely to affect the conductivity of streamer filaments and other long-lasting space charge structures like gigantic jets or the ionization state of the mesosphere when illuminated by thunderstorm fields. Detachment by itself does not directly affect small...

  1. Peripapillary subretinal neovascularization and serous macular detachment. Association with congenital optic nerve pits.

    Science.gov (United States)

    Borodic, G E; Gragoudas, E S; Edward, W O; Brockhurst, R J

    1984-02-01

    Congenital anomalous disc changes were associated with acquired macular detachment and peripapillary choroidal neovascularization in two cases. The anomalous disc changes resembled optic nerve pits. In one case, the peripapillary choroidal neovascularization was treated with argon laser photocoagulation, with subsequent reattachment of the macula and considerable improvement in the visual acuity. Although the pathogenesis of macular detachment occurring with optic nerve pits is usually not disclosed by fluorescein angiography, leakage from choroidal neovascularization can occur with this congenital defect and may contribute to the formation of a neurosensory macular detachment. If found, choroidal neovascularization may represent a remedial cause for visual loss in a condition with an otherwise poor prognosis.

  2. Delayed Descemet's membrane detachment after successful cataract surgery: a case report

    Directory of Open Access Journals (Sweden)

    Aileen Walsh

    2012-10-01

    Full Text Available The detachment of Descemet's membrane can be a serious complication following cataract surgery, leading to severe corneal edema and reduced visual acuity. This report describes an unusual case of Descemet's membrane detachment 6 months after successful phacoemulsification, documented by anterior segment optic coherence tomography (OCT; RTVue, Optovue. The eye was treated successfully with pneumatic descemetopexy and transcorneal suturing, with reattachment of Descemet's membrane. This report should alert physicians that delayed corneal edema can be related to late-onset Descemet's membrane detachment, which requires proper treatment to avoid permanent corneal decompensation.

  3. Reply to 'Calculation of depth to detachment and its significance in the Kuqa Depression: A discussion'

    Institute of Scientific and Technical Information of China (English)

    Yu Yixin; Tang Liangjie; He Chunbo; Chen Xuyun

    2009-01-01

    This paper mainly replies to the discussion by Li et al (2009) on the calculation of the detachment depths in the Kuqa Depression. We think that viscous material flowing into or out of the cross section can affect the validity of the calculation method, yet the number of detachment levels does not bring any drawbacks to the calculation. In the Kuqa Depression, the salt flow influenced the structural deformation to some extent, and affected slightly the accuracy of the calculated depths of the true detachment levels. However, it does not mean that the calculation method loses effectiveness in the study area. Therefore, the calculation results are still relatively believable.

  4. Rhegmatogenous retinal detachment in an eye of retinoblastoma treated by radiation and cryocoagulation

    Energy Technology Data Exchange (ETDEWEB)

    Inoue, Makoto; Tanaka, Yasuhiko; Kawai, Masataka; Nii, Seiji [Keio Univ., Tokyo (Japan). School of Medicine; Katsura, Hiroshi; Kaneko, Akihiro

    1999-08-01

    A 4-month-old female infant was diagnosed as bilateral retinoblastoma. The left eye was treated by enucleation and the right by radiation. The right eye received repeated thermochemotherapy, cryocoagulation and photocoagulation for recurrence. Total retinal detachment was detected after surgery for complicated cataract at the age of 2 years 9 months. Vitreous surgery led to the detection of posterior vitreous detachment and retinal breaks in the coagulated scar areas with vitreoretinal adhesion around the calcified tumor. During the following one year, the retina has been attached with no reoccurrence of the tumor. This case illustrates that rhegmatogenous retinal detachment is a possibility in retinoblastoma treated by radiation or retinal coagulations. (author)

  5. Scleral Buckling with and without Retinopexy and Laser Photocoagulation for Treatment of Rhegmatogenous Retinal Detachment

    Directory of Open Access Journals (Sweden)

    A Ghodosi

    2008-10-01

    Full Text Available Introduction: There are different methods to repair retinal detachment. In this trial the retinopexy in repairmen of retinal regmatogenesis RD is compared with other methods in Qazvin Teaching Hospitals during 1993-2003. Methods: This out come study was conducted on 47 patients with S/B and retinal break cryopexy (group I , 30 patients undergoing S/B with barrier laser photocoagulation around retinal breaks (group II and 41 patients with S/B without retinopexy (group III. An encircling or segmental buckle was placed for all eyes. In some patients, subretinal fluid drainage (SRED or anterior chamber paracentesis and intravitreal air dubble injection was performed. The patients were followed for 3 to 48 months. T. test, chi square and fisher exact test were used for data analysis. Results: The groups were similar with respect to age, sex, lens status, visual acuity, type, number and location of break. There were no differences in the surgical procedure performed. The reattachment rate in group I was 91.5% , in group II 86.7% and in group III was 97.6%, a difference that was not significant. Conclusions: With S/B procedure, retinal cryopexy does not seem to increase success rate of operation.

  6. Exclusive Use of Air as Gas Tamponade in Rhegmatogenous Retinal Detachment

    Science.gov (United States)

    Pak, Kang Yeun; Lee, Seok Jae; Kwon, Han Jo; Lee, Ji Eun

    2017-01-01

    Purpose To investigate outcomes of vitrectomy for rhegmatogenous retinal detachment (RRD) using air exclusively as the gas tamponade. Methods This retrospective, interventional, consecutive case series involved reviewing medical records of patients that underwent vitrectomy and gas tamponade for RRD between January 2013 and December 2015. Patients whose eyes were treated exclusively with air tamponade since July 2014 were assigned to the air group, while those treated with heterogeneous gas agents before June 2014 were assigned to the control group. The primary outcome was the primary reattachment rate. Best-corrected visual acuity (BCVA) and duration to detect redetachments were assigned as the secondary outcomes. Results The air group and the control group included 71 and 72 eyes, respectively. The primary reattachment rate was 94.4% in the air group and there was no significant difference with 94.4% in the control group (p = 0.951). BCVA was significantly better in the air group at 1 month (p = 0.021) but not at 3 months postoperatively (p = 0.561). Redetachments were recognized earlier in the air group (9.3 ± 0.5 days) compared with those in the control group (21.3 ± 7.4 days) (p = 0.041). Conclusions In cases of simple RRD with sufficient removal of subretinal fluid, air could be considered for use as gas tamponade. This trial is registered with KCT0002358. PMID:28785481

  7. Patterning bacterial communities on epithelial cells.

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    Mohammed Dwidar

    Full Text Available Micropatterning of bacteria using aqueous two phase system (ATPS enables the localized culture and formation of physically separated bacterial communities on human epithelial cell sheets. This method was used to compare the effects of Escherichia coli strain MG1655 and an isogenic invasive counterpart that expresses the invasin (inv gene from Yersinia pseudotuberculosis on the underlying epithelial cell layer. Large portions of the cell layer beneath the invasive strain were killed or detached while the non-invasive E. coli had no apparent effect on the epithelial cell layer over a 24 h observation period. In addition, simultaneous testing of the localized effects of three different bacterial species; E. coli MG1655, Shigella boydii KACC 10792 and Pseudomonas sp DSM 50906 on an epithelial cell layer is also demonstrated. The paper further shows the ability to use a bacterial predator, Bdellovibriobacteriovorus HD 100, to selectively remove the E. coli, S. boydii and P. sp communities from this bacteria-patterned epithelial cell layer. Importantly, predation and removal of the P. Sp was critical for maintaining viability of the underlying epithelial cells. Although this paper focuses on a few specific cell types, the technique should be broadly applicable to understand a variety of bacteria-epithelial cell interactions.

  8. Calpain-controlled detachment of major glycoproteins from the cytoskeleton regulates adhesive properties of activated phosphatidylserine-positive platelets.

    Science.gov (United States)

    Artemenko, Elena O; Yakimenko, Alena O; Pichugin, Alexey V; Ataullakhanov, Fazly I; Panteleev, Mikhail A

    2016-02-15

    In resting platelets, adhesive membrane glycoproteins are attached to the cytoskeleton. On strong activation, phosphatidylserine(PS)-positive and -negative platelet subpopulations are formed. Platelet activation is accompanied by cytoskeletal rearrangement, although the glycoprotein attachment status in these two subpopulations is not clear. We developed a new, flow cytometry-based, single-cell approach to investigate attachment of membrane glycoproteins to the cytoskeleton in cell subpopulations. In PS-negative platelets, adhesive glycoproteins integrin αIIbβ3, glycoprotein Ib and, as shown for the first time, P-selectin were associated with the cytoskeleton. In contrast, this attachment was disrupted in PS-positive platelets; it was retained to some extent only in the small convex regions or 'caps'. It correlated with the degradation of talin and filamin observed only in PS-positive platelets. Calpain inhibitors essentially prevented the disruption of membrane glycoprotein attachment in PS-positive platelets, as well as talin and filamin degradation. With the suggestion that detachment of glycoproteins from the cytoskeleton may affect platelet adhesive properties, we investigated the ability of PS-positive platelets to resist shear-induced breakaway from the immobilized fibrinogen. Shear rates of 500/s caused PS-positive platelet breakaway, but their adhesion stability increased more than 10-fold after pretreatment of the platelets with calpain inhibitor. In contrast, the ability of PS-positive platelets to adhere to immobilized von Willebrand's factor at 100/s was low, but this was not affected by the preincubation of platelets with a calpain inhibitor. Our data suggest that calpain-controlled detachment of membrane glycoproteins is a new mechanism that is responsible for the loss of ability of the procoagulant platelets to resist detachment from thrombi by high shear stress.

  9. From detachment to transtensional faulting: A model for the Lake Mead extensional domain based on new ages and correlation of subbasins

    Science.gov (United States)

    Beard, L.; Umhoefer, P. J.; Martin, K. L.; Blythe, N.

    2007-12-01

    New studies of selected basins in the Miocene extensional belt of the northern Lake Mead domain suggest a new model for the early extensional history of the region (lower Horse Spring Formation and correlative strata). Critical data are from (i) Longwell Ridges area west of Overton Arm and within the Lake Mead fault system, (ii) Salt Spring Wash basin in the hanging wall of the South Virgin-White Hills detachment (SVWHD) fault, and (iii) previously studied subbasins of the south Virgin Mountains in the Gold Butte step-over region. The basins and faulting patterns suggest two stages of basin development related to two distinct faulting episodes, an early period of detachment faulting followed by a switch to faulting mainly along the Lake Mead transtensional fault system while detachment faulting waned. Apatite fission track ages suggest the footwall block of the SVWHD was cooling at 18-17 Ma, but the only evidence for basin deposition at that time is in the Gold Butte step-over where slow rates of sedimentation and facies patterns make faulting on the north side of the Gold Butte block ambiguous. The first basin stage was ca. 16.5 to 15.5 Ma, during which there was slow to moderate faulting and subsidence in a basin along the SVWHD and north of Gold Butte block in the Gold Butte step-over basin; the step- over basin had complex fluvial and lacustrine facies and was synchronous with landslides and debris flows in front of the SVWHD. At ca. 15.5-14.5 Ma, there was a [dramatic] increase in sedimentation rate related to formation of the Gold Butte fault, a change from lacustrine to widespread fluvial, playa, and local landslide facies in the step-over basin, and the peak of exhumation and faulting rates on the SVWHD. The simple step-over basin broke up into numerous subbasins [at[ as initial faults of the Lake Mead fault system formed. From 14.5 to 14.0 Ma, there was completion of a major change from dominantly detachment faulting to dominantly transtensional faulting

  10. Bacterial survival rate on toothbrushes and their decontamination with antimicrobial solutions Taxa de sobrevivência bacteriana em escovas dentais e sua descontaminação com soluções antimicrobianas

    Directory of Open Access Journals (Sweden)

    Sandra Sato

    2004-06-01

    Full Text Available The purpose of this study was to evaluate bacterial survival rate on toothbrushes after brushing and the efficacy of their decontamination by spraying antimicrobial solutions. Thirty subjects were instructed to spray the solutions on toothbrush bristles after brushing. Each volunteer tested three sprays, one solution per week; the sprays were labeled spray 1 (cetylpyridinium chloride - CPC - and basic formulation, 2 (basic formulation only and 3 (control - sterile tap water. At the end of each week, the brushes were collected and sonicated in Letheen Broth®; the suspensions were ten-fold diluted and the dilutions were plated on various culture media. Anaerobic bacteria, evaluated by colony count of black pigment producing organisms on Ask medium, were recovered from 83.3% of the samples, Streptococci from 80% and aerobic Gram-negative bacilli from 46.7% of them in the control tests. There was a significant decrease in toothbrush contamination with antimicrobial sprays 1 and 2, the first showing the greatest decrease on bacterial counts.O propósito deste estudo foi avaliar a taxa de sobrevivência bacteriana em escovas dentais após a escovação e a eficácia na sua descontaminação pelo borrifamento de soluções antimicrobianas. Trinta indivíduos foram instruídos a borrifar as soluções nas cerdas das escovas após a escovação. Cada voluntário testou três sprays, uma solução por semana; os sprays foram rotulados spray 1 (cloreto de cetilpiridínio - CCP - e formulação básica, 2 (formulação básica apenas e 3 (controle - água de torneira esterilizada. Ao final de cada semana, as escovas eram recolhidas e introduzidas no caldo Letheen®, submetidas a ultra-som, à diluição decimal seriada e as suspensões semeadas em vários meios de cultura. As bactérias anaeróbias, avaliadas pela contagem de colônias de microrganismos produtores de pigmento negro no meio Ask, foram recuperadas em 83,3% das amostras, estreptococos em 80

  11. Comparisons of electronic and visual ratings of strawberry leaves inoculated with two colletotrichum species

    Science.gov (United States)

    Inoculation of detached strawberry leaves with Colletotrichum species may provide a rapid and accurate method for identifying anthracnose resistant germplasm. The purpose of this study was to compare electronic and visual rating methods of screening for anthracnose resistance. Eight to 17 detached...

  12. Aniseikonia and Foveal Microstructure after Retinal Detachment Surgery.

    Science.gov (United States)

    Okamoto, Fumiki; Sugiura, Yoshimi; Okamoto, Yoshifumi; Hiraoka, Takahiro; Oshika, Tetsuro

    2014-07-17

    Purpose:To quantify aniseikonia after successful surgical repair of rhegmatogenous retinal detachment (RD), and to investigate the relationship between the severity of postoperative aniseikonia and retinal microstructures as well as clinical parameters. Methods:The study included 106 eyes of 106 patients, without any history of ocular disease/surgery and less than 2 diopters of anisometropia, who had undergone successful retinal reattachment surgery. Aniseikonia was measured with New Aniseikonia Test and foveal microstructure was assessed with the spectral-domain optical coherence tomography at 6 months postoperatively. Results:Twenty-eight of 106 patients (26%) had micropsia, 17 patients (16%) had macropsia, and 61 patients (58%) had no aniseikonia. The mean absolute value of aniseikonia was 2.3 ± 2.9% (range; -12.5% - +12.0%). Of 57 eyes with macula-on RD, 3 had micropsia and 12 had macropsia. Of 49 eyes with macula-off RD, 25 had micropsia and 5 had macropsia. Eyes with micropsia mostly exhibited persistent or transient cystoid macular edema, subretinal fluid, hyperreflective or disruption of IS/OS line, while most of the eyes with macropsia presented epiretinal membrane. Stepwise multiple regression analysis revealed that postoperative best-corrected visual acuity and the area of RD were significantly relevant to the mean absolute value of aniseikonia. Conclusions:These results suggested that about half of patients with successful repair of RD had aniseikonia. Eyes with macula-off RD tended to show micropsia, while those with macula-on RD mostly presented macropsia. Micropsia and macropsia were primarily caused by respective specific abnormal structures at the foveal region.

  13. [Treatment of retinal detachment with a giant tear--personal observations concerning use of perfluorocarbon liquid].

    Science.gov (United States)

    Nawrocki, J; Wesołek, A; Pikulski, Z; Suprunowicz, I

    1994-01-01

    The authors present results of the treatment of retinal detachment with giant tear in 12 eyes in 11 patients. The encirclement of the eyeball, vitrectomy and endo-tamponade with gas in 3 cases and silicone oil in 9 were applied. In 4 cases, perfluorocarbon liquid was used to unroll the retina. After 6-15 months follow-up retina was completely attached in 8 eyes (66%); in 3 eyes (25%) residual detachment was observed in the inferior part of the fundus and in 1 eye retina remained detached. In 8 eyes, visual acuity was above 5/50, in 3 above 1/50. The type of endo-tamponade and the role of perfluorocarbon liquid in this kind of retinal detachment are discussed.

  14. [Rhegmatogenous retinal detachment in a patient with previous penetrating keratoplasty (clinical case)].

    Science.gov (United States)

    Burcea, M; Muşat, O; Gheorghe, Andreea; Mahdi, Labib; Colta, Diana; Cernat, Corina; Mansour, Agajani

    2014-01-01

    We present the case of a 54 year old patient diagnosed with rhegmatogenous retinal detachment and perforating keratoplasty. Surgery is recommended and we performed posterior vitrectomy, endolaser, and internal heavy oil tamponade. The post-operative course was favorable.

  15. Atypical presentation of ocular toxoplasmosis: A Case report of exudative retinal detachment and choroidal Ischemia

    Directory of Open Access Journals (Sweden)

    Yahya A Al-Zahrani

    2016-01-01

    Full Text Available A 24-year-old healthy male presented with a chief complaint of blurred vision in the right eye for 1-week. Fundus examination indicated right exudative retinal detachment and choroidal ischemia. The patient responded well to anti-toxoplasmosis medications and steroids. Exudative retinal detachment and choroidal ischemia are atypical presentations of ocular toxoplasmosis. However, both conditions responded well to anti.parasitic therapy with steroid.

  16. Atypical Presentation of Ocular Toxoplasmosis: A Case Report of Exudative Retinal Detachment and Choroidal Ischemia.

    Science.gov (United States)

    Al-Zahrani, Yahya A; Al-Dhibi, Hassan A; Al-Abdullah, Abdulelah A

    2016-01-01

    A 24-year-old healthy male presented with a chief complaint of blurred vision in the right eye for 1-week. Fundus examination indicated right exudative retinal detachment and choroidal ischemia. The patient responded well to anti-toxoplasmosis medications and steroids. Exudative retinal detachment and choroidal ischemia are atypical presentations of ocular toxoplasmosis. However, both conditions responded well to anti.parasitic therapy with steroid.

  17. Lamellar macular hole formation following vitrectomy for rhegmatogenous retinal detachment repair

    OpenAIRE

    Kabanarou SA; Feretis E; Xirou T; Kidess A; Xirou V; Kourentis C

    2012-01-01

    Tina Xirou, Andrej Kidess, Christina Kourentis, Vasiliki Xirou, Elias Feretis, Stamatina A KabanarouRetina Department, Hellenic Red Cross General Hospital, Athens, GreeceBackground: The purpose of this study was to investigate lamellar macular hole formation in six patients after rhegmatogenous retinal detachment repair.Methods: A retrospective review of medical records of patients who underwent primary pars plana vitrectomy for rhegmatogenous retinal detachment repair was performed. Optical ...

  18. Detachment of esophageal carcinoma cells from extracellular matrix causes relocalization of death receptor 5 and apoptosis

    Institute of Scientific and Technical Information of China (English)

    Guang-Chao Liu; Jun Zhang; Shi-Gui Liu; Rong Gao; Zhang-Fu Long; Ke Tao; Yuan-Fang Ma

    2009-01-01

    AIM:To investigate the effect of detachment of esophageal cancer cells from extracellular matrix on the localization of death receptor 5 (DR5) and apoptosis.METHODS: Anchorage-dependent EC9706 cells of esophageal squamous cell carcinoma were pretreated or not treated with brefeldin A. Detached cells were harvested by ethylenediaminetetraacetic acid digestion. Expression and localization of DR5 in these cells were determined by immunocytochemical and immunofluorescence assays, as well as flow cytometry analysis. Apoptosis of EC9706 cells was detected by flow cytometry after stained with fluorescein isothiocyanate-labeled annexin V/propidium iodide. Activation of caspase 8 was detected by Western blot analysis.RESULTS: Immunocytochemical assay indicated that DR5 was predominantly perinuclear in adherent cells but was mainly localized in cell membrane in detached cells. In addition, immunofluorescence assay also confirmed the above-mentioned results, and further demonstrated that DR5 was present in the form of coarse granules in detached cells, but in the form of fine granules in adherent cells. Cytometry analysis revealed higher levels of DR5 expression on the surfaces of brefeldin-A-untreated cells than on the surfaces of brefeldin-A-treated cells, but brefeldin A treatment did not affect the total DR5 expression levels. Moreover, nocodazole did not influence the extracelluar DR5 expression levels in EC9706 cells. Apoptosis assay revealed that detached cells were more sensitive to DR5 antibody-induced apoptosis than adherent cells. Western blotting showed that caspase 8 was activated in temporarily detached cells 4 h earlier than in adherent cells.CONCLUSION: Progress from adhesion to detachment of EC9706 cells causes DR5 relocalization, and promotes cytoplasmic translocation of DR5 to cell surfaces via a Golgi-dependent pathway. Moreover, it might also result in DR5 aggregation to render apoptosis of detached cells.

  19. Podocyte detachment and reduced glomerular capillary endothelial fenestration promote kidney disease in type 2 diabetic nephropathy

    OpenAIRE

    Weil, E. Jennifer; Lemley, Kevin V; Mason, Clinton C; Yee, Berne; Jones, Lois I.; Blouch, Kristina; Lovato, Tracy; Richardson, Meghan; Myers, Bryan D.; Nelson, Robert G.

    2012-01-01

    Podocyte detachment and reduced endothelial cell fenestration and relationships between these features and the classic structural changes of diabetic nephropathy have not been described in patients with type 2 diabetes. Here we studied these relationships in 37 Pima Indians with type 2 diabetes of whom 11 had normal albuminuria, 16 had microalbuminuria, and 10 had macroalbuminuria. Biopsies from ten kidney donors (not Americans Indians) showed almost undetectable (0.03%) podocyte detachment a...

  20. Slab detachment during continental collision: Influence of crustal rheology and interaction with lithospheric delamination

    Science.gov (United States)

    Duretz, T.; Gerya, T. V.

    2013-08-01

    Collision between continents can lead to the subduction of continental material. If the crust remains coupled to the downgoing slab, a large buoyancy force is generated. This force slows down convergence and promotes slab detachment. If the crust resists to subduction, it may decouple from the downgoing slab and be subjected to buoyant extrusion. We employ two-dimensional thermo-mechanical modelling to study the importance of crustal rheology on the evolution of subduction-collision systems. We propose simple quantifications of the mechanical decoupling between lithospheric levels (σ*) and the potential for buoyant extrusion of the crust (ξ*). The modelling results indicate that a variable crustal rheological structure results in slab detachment, delamination, or the combination of both mechanisms. A strong crust provides coupling at the Moho (low σ*) and remains coherent during subduction (low ξ). It promotes deep subduction of the crust (180 km) and slab detachment. Exhumation occurs in coherent manners via eduction and thrusting. Slab detachment triggers the development of topography (> 4.5 km) close to the suture. A contrasting style of collision occurs using a weak crustal rheology. Mechanical decoupling at the Moho (high σ*) promotes the extrusion of the crust (high ξ), disabling slab detachment. Ongoing shortening leads to buckling of the crust and development of topography on the lower plate. Collisions involving rheologically layered crust allow decoupling at mid-crustal depths. This structure favours both the extrusion of upper crust and the subduction of the lower crust. Such collisions are successively affected by delamination and slab detachment. Topography develops together with the buoyant extrusion of crust onto the foreland and is further amplified by slab detachment. Our results suggest that the occurrence of both delamination (Apennines) and slab detachment (Himalayas) in orogens may indicate differences in the initial crustal structure of

  1. Repair of very severe tricuspid regurgitation following detachment of the tricuspid valve

    OpenAIRE

    Mahgoub, Ahmed; Kamel, Hassan; Simry, Walid; Hosny, Hatem

    2015-01-01

    We report on the case of 5-year-old girl with severe tricuspid regurgitation following previous repair of double outlet right ventricle with subaortic ventricular septal defect, performed through trans-atrial approach using detachment of tricuspid valve leaflet. The severe tricuspid regurgitation was found to be due to dehiscence at the site of the previous detachment and was repaired using a pericardial patch. In this report, we discuss the relative merits and risks of using this technique.

  2. Inicial nasal mucosa detachment using piezoelectric device in the Le Fort I osteotomy: A technical note

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    Elio Hitoshi Shinohara

    2013-01-01

    Full Text Available In maxillary Le Fort I type osteotomy the detachment of the nasal mucosa should be done carefully. Piezoelectric surgery contributed much to increase the safety of osteotomies, despite the initial advantage of minimizing the risk of injury in nervous tissue, mainly in bilateral sagittal split osteotomy; we use the piezoelectric device for the initial detachment of the nasal mucosa in the maxillary osteotomy.

  3. Bacterial vaginosis and preterm birth.

    Science.gov (United States)

    Manns-James, Laura

    2011-01-01

    Although it has been clear for more than 2 decades that bacterial vaginosis increases the risk for preterm birth in some women, it is not yet fully understood why this association exists or how best to modify the risk. Incomplete understanding of this polymicrobial condition and difficulties in classification contribute to the challenge. The relationship between altered vaginal microflora and preterm birth is likely mediated by host immune responses. Because treatment of bacterial vaginosis during pregnancy does not improve preterm birth rates, and may in fact increase them, screening and treatment of asymptomatic pregnant women is discouraged. Symptomatic women should be treated for symptom relief. This article reviews the pathophysiology of bacterial vaginosis and controversy surrounding management during pregnancy. Agents currently recommended for treatment of this condition are reviewed. © 2011 by the American College of Nurse-Midwives.

  4. Keratinocyte detachment-differentiation connection revisited, or anoikis-pityriasi nexus redux.

    Science.gov (United States)

    Banno, Tomohiro; Blumenberg, Miroslav

    2014-01-01

    Epidermis, a continuously self-renewing and differentiating organ, produces a protective stratum corneum that shields us from external chemical, physical and microbial threats. Epidermal differentiation is a multi-step process regulated by influences, some unknown, others insufficiently explored. Detachment of keratinocytes from the basement membrane is one such pro-differentiation stimulus. Here, we define the transcriptional changes during differentiation, especially those caused by detachment from the substratum. Using comprehensive transcriptional profiling, we revisited the effects of detachment as a differentiation signal to keratinocytes. We identified the genes regulated by detachment, the corresponding ontological categories and, using metaanalysis, compared the genes and categories to those regulated by other pro-differentiating stimuli. We identified 762 genes overexpressed in suspended keratinocyte, including known and novel differentiation markers, and 1427 in attached cells, including basal layer markers. Detachment induced epidermis development, cornification and desmosomal genes, but also innate immunity, proliferation inhibitors, transcription regulators and MAPKs; conversely the attached cells overexpressed cell cycle, anchoring, motility, splicing and mitochondrial genes, and both positive and negative regulators of apoptosis. Metaanalysis identified which detachment-regulated categories overlap with those induced by suprabasal location in vivo, by reaching confluency in vitro, and by inhibition of JUN kinases. Attached and in vivo basal cells shared overexpression of mitochondrial components. Interestingly, melanosome trafficking components were also overexpressed in the attached and in vivo basal keratinocytes. These results suggest that specific pro-differentiation signals induce specific features of the keratinization process, which are in vivo orchestrated into harmonious epidermal homeostasis.

  5. An unusual case of bilateral multifocal retinal pigment epithelial detachment with methanol-induced optic neuritis.

    Science.gov (United States)

    Ranjan, Ratnesh; Kushwaha, Rajnath; Gupta, Ramesh Chandra; Khan, Perwez

    2014-03-01

    To describe an unusual case of methanol-induced optic neuritis with bilateral multifocal extrafoveal serous retinal pigment epithelial (RPE) detachment. Single case report. A 40-year-old male presented with acute bilateral loss of vision and history of consumption of adulterated alcohol. On examination, his vision was perception of light in the right eye and finger counting at 1-ft distance in the left eye. Pupillary reactions were sluggish. The optic discs were normal. An elevated lesion with subretinal serous fluid was present over macula adjacent to superior major vessel arcade in the right eye, which was confirmed as a large extrafoveal RPE detachment on fluorescein angiography. There were two more small RPE detachments in the right eye as well as in the left eye. All RPE detachments were extrafoveal in location. The patient was managed medically with intravenous methylprednisolone (1 g) in 500 ml of ringer lactate for three consecutive days. After three doses, visual acuity of both eyes was recorded as 20/20. We herein report an unusual case of bilateral multifocal extrafoveal serous RPE detachment in a patient of methanol-induced optic neuritis. RPE detachments may be due to the toxic effect of methanol metabolites.

  6. Lamellar macular hole formation following vitrectomy for rhegmatogenous retinal detachment repair

    Directory of Open Access Journals (Sweden)

    Kabanarou SA

    2012-04-01

    Full Text Available Tina Xirou, Andrej Kidess, Christina Kourentis, Vasiliki Xirou, Elias Feretis, Stamatina A KabanarouRetina Department, Hellenic Red Cross General Hospital, Athens, GreeceBackground: The purpose of this study was to investigate lamellar macular hole formation in six patients after rhegmatogenous retinal detachment repair.Methods: A retrospective review of medical records of patients who underwent primary pars plana vitrectomy for rhegmatogenous retinal detachment repair was performed. Optical coherence tomography characteristics and best-corrected visual acuity were evaluated. Patients who developed lamellar macular hole after pars plana vitrectomy for rhegmatogenous retinal detachment repair were identified.Results: A total of 1185 eyes underwent pars plana vitrectomy for retinal detachment between 2004 and 2009. Optical coherence tomography evaluation was available in 450 cases. Six of these cases demonstrated lamellar macular hole formation, which was diagnosed by OCT-3. The mean time from retinal detachment surgery to lamellar hole diagnosis was 4.1 months. The presence of an epiretinal membrane on the surface of the juxtafoveal retina was a common finding in all six patients. Visual acuity was improved after successful retinal reattachment and remained unchanged after lamellar hole formation.Conclusion: Lamellar macular holes developing after pars plana vitrectomy is a rare complication. Stability of optical coherence tomography findings and best-corrected visual acuity after lamellar macular hole formation may be observed for at least two years.Keywords: lamellar macular hole, rhegmatogenous retinal detachment

  7. Effects of temporal muscle detachment and coronoidotomy on facial growth in young rats

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    Fernanda Engelberg Fernandes Gomes

    2012-08-01

    Full Text Available This study analyzed the effects of unilateral detachment of the temporal muscle and coronoidotomy on facial growth in young rats. Thirty one-month-old Wistar rats were distributed into three groups: detachment, coronoidotomy and sham-operated. Under general anesthesia, unilateral detachment of the temporal muscle was performed for the detachment group, unilateral coronoidotomy was performed for the coronoidotomy group, and only surgical access was performed for the sham-operated group. The animals were sacrificed at three months of age. Their soft tissues were removed, and the mandible was disarticulated. Radiographic projections-axial views of the skulls and lateral views of hemimandibles-were taken. Cephalometric evaluations were performed, and the values obtained were submitted to statistical analyses. There was a significant homolateral difference in the length of the premaxilla, height of the mandibular ramus and body, and the length of the mandible in all three groups. However, comparisons among the groups revealed no significant differences between the detachment and coronoidotomy groups for most measurements. It was concluded that both experimental detachment of the temporal muscle and coronoidotomy during the growth period in rats induced asymmetry of the mandible and affected the premaxilla.

  8. The temporal dynamics of detached versus positive reappraisal: An ERP study.

    Science.gov (United States)

    Qi, Senqing; Li, Yangping; Tang, Xuemei; Zeng, Qinghong; Diao, Liuting; Li, Xiying; Li, Hong; Hu, Weiping

    2017-01-23

    Detached reappraisal and positive reappraisal are regarded as two distinct types of cognitive reappraisal strategy, and the former is considered more disengaging than the latter. The conceptual framework of emotion regulation choice posits that strategies involving disengagement operate earlier and more efficiently than engagement strategies. Here, we compare for the first time the temporal dynamics of detached and positive reappraisal during the regulation and re-exposure phases thereof by measuring event-related potentials. During the regulation phase, pictures were viewed or regulated using detached or positive reappraisal. During the re-exposure phase, the same pictures were viewed again. Results showed that during regulation, central-parietal late positive potentials (LPPs) were greatly attenuated under both strategy types, with the regulation effect of detached reappraisal occurring earlier than that of positive reappraisal and resulting in a stronger attenuation of LPP amplitudes. Upon re-exposure, detached reappraisal exerted enduring effects on self-reported arousal and the central-parietal LPP, whereas positive reappraisal had an enduring effect only on pleasantness. These findings demonstrate the differential effects of detached and positive reappraisal on valence, arousal, and neural responses, and underline the striking differences in the temporal dynamics of these reappraisal strategies.

  9. Posterior vitreous cortex contributes to macular hole in highly myopic eyes with retinal detachment

    Institute of Scientific and Technical Information of China (English)

    LIU Hai-yun; ZOU Hai-dong; LIU Kun; SONG Zheng-yu; XU Xun; SUN Xiao-dong

    2011-01-01

    Background It was well known that tangential vitreoretinal traction and epiretinal membrane play important roles during the formation of macular hole (MH) associated with retinal detachment (RD) in highly myopic eyes. But it was not clear about the correlations between anteroposterior traction, posterior vitreous cortex (PVC) and MH-RD. The vitreous status in highly myopic eyes were analyzed to explore the effect of PVC in the role of MH-RD formation.Methods Sixteen consecutive highly myopic eyes with RD due to MH were retrospectively analyzed from January 2009 to April 2009. The preoperative examinations for detecting posterior vitreous detachment (PVD) and vitreoretinal traction included B-mode ultrasonography and optical coherence tomography (OCT). The residual PVC and PVD were confirmed intraoperatively during triamcinolone acetonide (TA) assisted vitrectomy.Results Under ultrasonography, the preoperative PVD patterns were stratified as: complete PVD in three (19%) eyes,partial PVD in eight (50%) eyes, and no PVD in five (31%) eyes. OCT confirmed vitreoretinal traction and no complete PVD in 10 (63%) eyes, including anteroposterior traction in four eyes and tangential traction in six eyes. During TA-assisted vitrectomy, it was confirmed that no complete PVD existed in 16 eyes, including six eyes (38%) finally diagnosed of partial PVD, and five (31%) eyes with vitreoschisis. Anteroposterior vitreoretinal traction around MH is always in conjunction with partial PVD (67%), and high proportion (80%) of vitreoschisis is associated with tangential vitreoretinal traction. Comparing with the precision of TA staining of PVD diagnosis, the coincidence rate of ultrasonography was 69% (P=-0.02), and that of OCT was 63% (P <0.01).Conclusions The residual PVC due to partial PVD or vitreoschisis may cause the anteroposterior or tangential traction of macular area, which contributes to the formation of MH and subsequent RD in highly myopic eyes. And it is necessary to

  10. Spontaneous bacterial peritonitis

    Institute of Scientific and Technical Information of China (English)

    Anastasios Koulaouzidis; Shivaram Bhat; Athar A Saeed

    2009-01-01

    Since its initial description in 1964, research has transformed spontaneous bacterial peritonitis (SBP) from a feared disease (with reported mortality of 90%) to a treatable complication of decompensated cirrhosis,albeit with steady prevalence and a high recurrence rate. Bacterial translocation, the key mechanism in the pathogenesis of SBP, is only possible because of the concurrent failure of defensive mechanisms in cirrhosis.Variants of SBP should be treated. Leucocyte esterase reagent strips have managed to shorten the 'tap-toshot' time, while future studies should look into their combined use with ascitic fluid pH. Third generation cephalosporins are the antibiotic of choice because they have a number of advantages. Renal dysfunction has been shown to be an independent predictor of mortality in patients with SBP. Albumin is felt to reduce the risk of renal impairment by improving effective intravascular volume, and by helping to bind proinflammatory molecules. Following a single episode of SBP, patients should have long-term antibiotic prophylaxis and be considered for liver transplantation.

  11. Electric field effects on the dynamics of bubble detachment from an inclined surface

    Science.gov (United States)

    Di Marco, P.; Morganti, N.; Saccone, G.

    2015-11-01

    An experimental apparatus to study bubble detachment from an inclined surface under the action of electric forces is described. It consists of a container filled with FC72 at room temperature and pressure where a train of gas bubbles is injected from an orifice. An electrostatic field can be imposed around the bubble, while the cell can be tilted from 0 to 90°. It is possible to study interface growth with the aid of high-speed cinematography. Since the interface is asymmetrical, a mirror system allowed to acquire, in the same frame, two images at 90° of the bubble. Different inclinations, injection rates and voltages were tested in order to couple the effects of shear gravity and electric field. Curvature and contact angles have been derived with appropriate interpolation methods of the profile. Force balances on the bubble were checked, finding an electric force, which, at first pulls the bubbles from the orifice, then pushes it against the surface. The motion of the center of gravity confirms this behaviour. A power balance has been developed to determine the energy contributions, revealing that surface growth incorporates both the effects of inlet power and electric field.

  12. District heating and energy efficiency in detached houses of differing size and construction

    Energy Technology Data Exchange (ETDEWEB)

    Joelsson, Anna; Gustavsson, Leif [Ecotechnology, Department of Engineering, Physics and Mathematics, Mid Sweden University, SE-831 25 Oestersund (Sweden)

    2009-02-15

    House envelope measures and conversion of heating systems can reduce primary energy use and CO{sub 2} emission in the existing Swedish building stock. We analysed how the size and construction of electrically heated detached houses affect the potential for such measures and the potential for cogenerated district heating. Our starting point was two typical houses built in the 1970s. We altered the floor plans to obtain 6 houses, with heated floor space ranging between 100 and 306 m{sup 2}. One of the houses was also analysed for three energy standards with differing heat loss rates. CO{sub 2} emission, primary energy use and heating cost were estimated after implementing house envelope measures, conversions to other heating systems and changes in the generation of district heat and electricity. The study accounted for primary energy, including energy chains from natural resources to useful heat in the houses. We showed that conversion to district heating based on biomass, together with house envelope measures, reduced the primary energy use by 88% and the CO{sub 2} emission by 96%, while reducing the annual societal cost by 7%. The choice of end-use heating system was decisive for the primary energy use, with district heating being the most efficient. Neither house size nor energy standard did significantly change the ranking of the heating systems, either from a primary energy or an economic viewpoint, but did affect the extent of the annual cost reduction after implementing the measures. (author)

  13. Combined 23-gauge sutureless vitrectomy and clear corneal phacoemulsification for rhegmatogenous retinal detachment repair

    Institute of Scientific and Technical Information of China (English)

    Hoseok; Moon; Hee; Jin; Sohn; Dea; Yeong; Lee; Jong; Yeon; Lee; Dong; Heun; Nam

    2015-01-01

    AIM: To assess the outcomes of combined 23-gauge sutureless vitrectomy and clear corneal phacoemulsification with intraocular lens implantation for rhegmatogenous retinal detachment(RRD) repair.METHODS: This was a retrospective, consecutive,noncomparative, interventional case series of 30 eyes of30 patients who underwent combined sutureless vitrectomy and clear corneal cataract surgery for the repair of RRD. The principal outcome measures were primary anatomical success rate, reasons for redetachment, final visual acuity, and surgical complications.RESULTS: Primary reattachment was achieved in 27eyes(90.0%). The reasons for redetachment(3 eyes, 10%)were incomplete laser retinopexy, persistent chronic subretinal fluid, and proliferative vitreoretinopathy,respectively. The logarithm of the minimum angle of resolution visual acuity(mean±SD) improved from 0.76±0.74 preoperatively to 0. 21 ± 0. 37 6 months’ postoperatively(P <0.0001). Postoperative hypotony was not detected,but 1 eye( 3. 3 %) had increased intraocular pressure(30 mm Hg) with spontaneous resolution. No endophthalmitis developed during follow-up. Macular pucker was detected in 3 eyes(10.0%).· CONCLUSION: Combined 23-gauge sutureless vitrectomy and clear corneal phacoemulsification with intraocular lens implantation for RRD repair was proven safe and effective. It may provide not only the known advantages of conventional combined surgery, but also additional advantages such as less conjunctival fibrosis and the maintenance of stable intraocular pressure with low risks of postoperative hypotony and intraocular pressure elevation.

  14. Accretion in the detached post-common-envelope binary LTT 560

    CERN Document Server

    Tappert, C; Schmidtobreick, L; Ribeiro, T

    2011-01-01

    In a previous study, we found that the detached post-common-envelope binary LTT 560 displays an Halpha emission line consisting of two anti-phased components. While one of them was clearly caused by stellar activity from the secondary late-type main-sequence star, our analysis indicated that the white dwarf primary star is potentially the origin of the second component. However, the low resolution of the data means that our interpretation remains ambiguous. We here use time-series UVES data to compare the radial velocities of the Halpha emission components to those of metal absorption lines from the primary and secondary stars. We find that the weaker component most certainly originates in the white dwarf and is probably caused by accretion. An abundance analysis of the white dwarf spectrum yields accretion rates that are consistent with mass loss from the secondary due to a stellar wind. The second and stronger Halpha component is attributed to stellar activity on the secondary star. An active secondary is l...

  15. Detached Concern of Forensic Mental Health Nurses in Therapeutic Relationships With Patients: The Application of the Early Recognition Method Related to Detached Concern

    NARCIS (Netherlands)

    Fluttert, F.A.J.; Meijel, B.K.G. van; Nijman, H.L.I.; Björkly, S.; Grypdonck, M.H.F.

    2010-01-01

    Objective - Improvement of the interaction between forensic mental health nurses and patients may lead to a reduction of inpatient violence. The concept under study is detached concern, which refers to nurses' skills to neutralize the emotional appeal of patients by a balanced attitude between objec

  16. Detached Concern of Forensic Mental Health Nurses in Therapeutic Relationships With Patients: The Application of the Early Recognition Method Related to Detached Concern

    NARCIS (Netherlands)

    Fluttert, F.A.J.; Meijel, B.K.G. van; Nijman, H.L.I.; Björkly, S.; Grypdonck, M.H.F.

    2010-01-01

    Objective - Improvement of the interaction between forensic mental health nurses and patients may lead to a reduction of inpatient violence. The concept under study is detached concern, which refers to nurses' skills to neutralize the emotional appeal of patients by a balanced attitude between

  17. Association Between Oral Fluoroquinolone Use and Retinal Detachment.

    Science.gov (United States)

    Raguideau, Fanny; Lemaitre, Magali; Dray-Spira, Rosemary; Zureik, Mahmoud

    2016-04-01

    Several studies have focused on the current use of oral fluoroquinolones and the risk for retinal detachment (RD), but the existence of this association is under debate. Given the widespread fluoroquinolone use, investigation of this association is essential. To assess the association between oral fluoroquinolone use and the risk for RD, including the rhegmatogenous and exudative types. This case-crossover study included 27,540 adults with RD from French health care databases from July 1, 2010, through December 31, 2013. Patients with a history of RD or retinal break, endophthalmitis, intravitreal injection, choroidal retinal vitreal biopsy, and human immunodeficiency virus infection or those hospitalized within 6 months of RD were excluded. The risk period of primary interest was current use, defined as exposure to fluoroquinolones within 10 days immediately before RD surgery, according to previous findings. Oral fluoroquinolone use was assumed to start on the day the prescription was dispensed. Exposure to fluoroquinolones during the risk period (1-10 days) compared with the control period (61-180 days). The association was also assessed regarding use in the recent (11-30 days) and past (31-60 days) intermediate risk period, type of fluoroquinolone, and type of RD. Of the 27,540 eligible patients (57% men; mean [SD] age, 61.5 [13.6] years), 663 patients with RD were exposed to fluoroquinolones during the observation period, corresponding to 80 cases exposed during the 10-day risk period (≤10 days before RD) and 583 cases exposed during the control period (61-180 days). We found a significant increased risk for RD during the 10-day period after the dispensing of oral fluoroquinolones, with an adjusted odds ratio of 1.46 (95% CI, 1.15-1.87). The risk was significantly increased for rhegmatogenous and exudative RD, with adjusted odds ratios of 1.41 (95% CI, 1.04-1.92) and 2.57 (95% CI, 1.46-4.53), respectively. Recent and past use of fluoroquinolones were not

  18. Probiotics--a helpful additional therapy for bacterial vaginosis

    National Research Council Canada - National Science Library

    Bodean, O; Munteanu, O; Cirstoiu, C; Secara, D; Cirstoiu, M

    2013-01-01

    Bacterial vaginosis is a condition of unknown etiology, associated with an imbalance of the normal vaginal microbiota, characterized by a high recurrence rate despite of classical therapy solutions...

  19. A new structural model of the Pachitea Basin, Peru: Interaction of thick-skinned tectonics and salt detached thrusting

    Science.gov (United States)

    Witte, J.; Rebaza, J.; Westlund, D.; Stratton, M.; Alegria, C.

    2015-11-01

    We present four new structural transects, a new seismo-stratigraphic correlation, a refined structural model and new shortening rates for the Pachitea Basin (=PB), Peru. Our results are based on the integration and detailed interpretation of newly acquired industry seismic (2D, 2005 vintage), existing well data, existing and proprietary surface geology data and newly acquired aero magnetic data (2007 vintage). Our assessment confirms the presence of at least four distinct structural styles in the area, thick-skinned structures, thin-skinned detachment thrusting, salt-tectonics and localized strike-slip tectonics. Based on seismo-stratigraphic correlations we conclude that the oldest rocks carried to outcrop by the San Matias (=SM) thrust are of Jurassic age. We interpret the thin-skinned master detachment to be located in varying positions, directly below or above, autochtonous salt pillows. Timing assessment of the SM thrust sheet reveals that it has been active from at least ˜5 Ma to post-2 Ma, supporting regionally published timing data for this latitude. Positive topographic surface expressions indicate ongoing contraction along the mountain front of the Peruvian Eastern Cordillera (=EC). Across the PB we calculate between 2.6% and 5.5% for thick-skinned shortening and at least 25.5% for the thin-skinned shortening. For the SM thrust sheet we calculate a slip-rate of ˜1-1.6 mm/yr, which is in line with published slip rates on individual thrusts from around the world. Observations along the SM thrust system indicate that thin- and thick-skinned systems interact mechanically, and that they have been active intermittently. We conclude that the location of salt pillows as well as pre-existing or growing basement-involved structures helped trigger the SM thrust. Different types of salt bodies are present in the PB, autochtonous pillows, slightly thrusted pillows and allochtonous diapirs. Our results provide new insight into the structural interplay, particularly

  20. Fluid flow along North American Cordillera detachments determined from stable isotope and high resolution chemical analyses

    Science.gov (United States)

    Quilichini, A.; Teyssier, C.; Mulch, A.; Nachlas, W.

    2009-12-01

    Fluid flow is likely a major parameter controlling the dynamics of extensional detachment zones. Buoyancy-driven fluid flow is generated by high heat flow beneath the detachment zone, where heat is advected by crustal thinning and magma intrusions. This hydrothermal convective flow is focused in the detachment zone for the duration of activity of the detachment at relatively high temperature (300-500°C), resulting in very significant fluid-rock interaction and isotopic exchange. Quantifying sources and fluid flux in detachments is a challenge because permeability of ductilely deforming rocks is poorly understood. In order to solve these problems, we studied two different Eocene extensional systems in the North American Cordillera: the quartzitic detachment which borders the Kettle dome metamorphic core complex (WA), and the quartzo-feldspathic Bitterroot shear zone along the Idaho batholith (MT). The Kettle Dome detachment provides a continuous section of ~200 m thick quartzite mylonite where high-resolution sampling (~5 m) indicates that Deuterium isotopic ratios that are obtained from synkinematic muscovite grains are consistent with a meteoric fluid source (-130 per mil). In the Bitterroot shear zone, Coyner (2003) reported similar Deuterium isotopic ratios (down to -140 per mil) in muscovite from mylonites and ultramylonites. Microprobe analyses were obtained for white mica porphyroclasts by performing transects perpendicular to the basal (001) cleavage in order to determine intragrain chemical zoning. Preliminary results for the Kettle dome indicate increasing phengite composition with depth, suggesting enhanced activity of the Tschermak exchange. The variations of the phengitic signature in muscovite indicates that temperature diminuish downsection, which is contradictory with the results obtained by the Qz-Ms oxygen isotope thermometer along the Kettle section. Our recent work provides geologic data for numerical models that address the permeability of

  1. Prevention of bacterial adhesion

    DEFF Research Database (Denmark)

    Klemm, Per; Vejborg, Rebecca Munk; Hancock, Viktoria

    2010-01-01

    Management of bacterial infections is becoming increasingly difficult due to the emergence and increasing prevalence of bacterial pathogens that are resistant to available antibiotics. Conventional antibiotics generally kill bacteria by interfering with vital cellular functions, an approach...... that imposes selection pressure for resistant bacteria. New approaches are urgently needed. Targeting bacterial virulence functions directly is an attractive alternative. An obvious target is bacterial adhesion. Bacterial adhesion to surfaces is the first step in colonization, invasion, and biofilm formation....... As such, adhesion represents the Achilles heel of crucial pathogenic functions. It follows that interference with adhesion can reduce bacterial virulence. Here, we illustrate this important topic with examples of techniques being developed that can inhibit bacterial adhesion. Some of these will become...

  2. Mass transfer and loss of the massive semi-detached binary AI Crucis

    Institute of Scientific and Technical Information of China (English)

    Er-Gang Zhao; Sheng-Bang Qian; E.Fernández Lajús; Carolina von Essen; Li-Ying Zhu

    2010-01-01

    AI Crucis is a short-period semi-detached massive close binary (P =1.41771d,Sp.=B 1.5) in the open cluster NGC 4103. It is a good astrophysical laboratory for investigating the formation and evolution of massive close binary stars via case A mass transfer. Orbital period variations of the system were analyzed based on one newly determined eclipse time and the others compiled from the literature. It is discovered that the orbital period of the binary is continuously increasing at a rate of dP/dt = +1.00(+0.04) x 10-7 d yr-1. After the long-term increase is subtracted from the O-C diagram,weak evidence indicates the presence of a cyclic oscillation with a period of 30.1 yr,which may reveal a very cool stellar companion in the system. The long-term period increase can be explained by mass transfer from the less massive component to the more massive one. This is in agreement with the semidetached configuration of the binary,indicating that the system is undergoing a slow mass-transfer stage on the nuclear time scale of the secondary. However,it is found that the slow mass transfer is insufficient to cause the observed period increase,which suggests that the stellar wind from the hot component should contribute to the amount of period increase dP/dt = +0.54×10-7 d yr-1 that corresponds to a mass loss rate of M1 = 2.72×10-7 M⊙yr-1. It is estimated that the hot component lost a total mass of 4.1 M⊙ during the slow mass-transfer stage and,thus,the evolution of the binary system should be changed greatly by the mass loss.

  3. Refraining from intrusive thoughts is strategy dependent: a comment on Sugiura, et al. And a preliminary informal test of detached mindfulness, acceptance, and other strategies.

    Science.gov (United States)

    Wells, Adrian; Roussis, Panagiotis

    2014-10-01

    The control of cognition is fundamental to psychological well being. One dimension recently explored by Sugiura, Sugiura and Tanno (2013) is the perceived ability to refrain from catastrophic thinking-a construct that could be a marker of several factors. The current paper recommends deeper consideration in terms of metacognitive theory and exemplifies this by testing the effect of a strategy that focuses on abstaining from processes (detached mindfulness) vs. transforming content (acceptance, brief exposure). Fifty-six participants (M age = 21.5 yr., range = 18-42) were randomly assigned to detached mindfulness, acceptance, exposure, or a control group before watching a stressful film that induced intrusive images. Afterwards, they engaged in their respective strategies for 5 min. and the frequency of intrusive images was rated. Detached mindfulness was the only manipulation that was associated with a statistically significant lower frequency of intrusions than the control condition. It is argued that assessment of perceived skills to refrain from thinking should be conceptualized within a metacognitive framework that distinguish process- and content-oriented strategies and address the question: When is a strategy a true refrain?

  4. Absorption and metabolism of formaldehyde in solutions by detached banana leaves.

    Science.gov (United States)

    Zeng, Zhidong; Qi, Chuanjiao; Chen, Qi; Li, Kunzhi; Chen, Limei

    2014-05-01

    Detached banana leaves are one of the by-products of banana production. In this study, the absorption and metabolism of formaldehyde (HCHO) in solutions by detached banana leaves was investigated under submergence conditions. The results showed that banana leaves could effectively absorb HCHO in the treatment solutions, and the relationship between HCHO absorption and treatment time appeared to fit a radical root function model. (13)C nuclear magnetic resonance analysis was used to investigate the ability of detached banana leaves to metabolise H(13)CHO, and the results indicated that the H(13)CHO absorbed from the treatment solutions was converted into non-toxic compounds. High amounts of [U-(13)C]glucose, [U-(13)C]fructose, [3-(13)C]serine and [3-(13)C]citrate were produced as a result of H(13)CHO metabolism in banana leaves, and the production of a small amount of [2,4-(13)C]citrate and [2,3-(13)C]alanine was also observed. These results suggest that detached banana leaves can metabolise H(13)CHO and convert it to non-toxic compounds. The metabolic pathways that produce these intermediates in detached banana leaves are postulated based on our (13)C nuclear magnetic resonance data.

  5. Interactions between plutonism and detachments during metamorphic core complex formation, Serifos Island (Cyclades, Greece)

    Science.gov (United States)

    Rabillard, Aurélien; Arbaret, Laurent; Jolivet, Laurent; Le Breton, Nicole; Gumiaux, Charles; Augier, Romain; Grasemann, Bernhard

    2015-06-01

    In order to better understand the interactions between plutonic activity and strain localization during metamorphic core complex formation, the Miocene granodioritic pluton of Serifos (Cyclades, Greece) is studied. This pluton (11.6-9.5 Ma) intruded the Cycladic Blueschists during thinning of the Aegean domain along a system of low-angle normal faults belonging to the south dipping West Cycladic Detachment System (WCDS). Based on structural fieldwork, together with microstructural observations and anisotropy of magnetic susceptibility, we recognize a continuum of deformation from magmatic to brittle conditions within the magmatic body. This succession of deformation events is kinematically compatible with the development of the WCDS. The architecture of the pluton shows a marked asymmetry resulting from its interaction with the detachments. We propose a tectonic scenario for the emplacement of Serifos pluton and its subsequent cooling during the Aegean extension: (1) A first stage corresponds to the metamorphic core complex initiation and associated southwestward shearing along the Meghàlo Livadhi detachment. (2) In the second stage, the Serifos pluton has intruded the dome at shallow crustal level, piercing through the ductile/brittle Meghàlo Livadhi detachment. Southwest directed extensional deformation was contemporaneously transferred upward in the crust along the more localized Kàvos Kiklopas detachment. (3) The third stage was marked by synmagmatic extensional deformation and strain localization at the contact between the pluton and the host rocks resulting in nucleation of narrow shear zones, which (4) continued to develop after the pluton solidification.

  6. Vitrectomy for optic disc pit-associated maculopathy with or without preoperative posterior vitreous detachment

    Directory of Open Access Journals (Sweden)

    Haruta M

    2012-08-01

    Full Text Available Masatoshi Haruta, Rika Kamada, Yumi Umeno, Ryoji YamakawaDepartment of Ophthalmology, Kurume University School of Medicine, Kurume, Fukuoka, JapanBackground: The purpose of this study was to evaluate the efficacy of pars plana vitrectomy for the treatment of optic disc pit-associated maculopathy with or without preoperative posterior vitreous detachment.Methods: We reviewed the clinical records of four consecutive patients who underwent pars plana vitrectomy in one eye for the treatment of optic disc pit-associated maculopathy, with an emphasis on the preoperative condition of the posterior hyaloid membrane.Results: Two of four eyes were confirmed to have an attached posterior hyaloid membrane, which was subsequently removed during surgery. Following vitrectomy, these two eyes experienced an improvement in visual acuity with complete retinal attachment of the macula. However, the other two eyes, which already had a posterior vitreous detachment at the time of surgery, showed a decrease in visual acuity with persistent maculopathy postoperatively.Conclusion: Pars plana vitrectomy for optic disc pit-associated maculopathy was beneficial for improving visual acuity in two eyes without preoperative posterior vitreous detachment but not in two eyes with preoperative posterior vitreous detachment. Our study suggests that preoperative assessment of a posterior hyaloid membrane is clinically important in predicting the surgical outcome of optic disc pit-associated maculopathy.Keywords: gas tamponade, internal limiting membrane, macular hole, optical coherence tomography, posterior hyaloid membrane, retinal detachment

  7. MgS in detached shells around carbon stars. Mining the mass-loss history

    CERN Document Server

    Hony, S

    2004-01-01

    We investigate the dust composition of detached shells around carbon stars, with a focus to understand the origin of the cool magnesium-sulfide (MgS) material around warm carbon stars, which has been detected around several of these objects. We build a radiative transfer model of a carbon star surrounded by an expanding detached shell of dust. The shell contains amorphous carbon grains and MgS grains. We find that a small fraction of MgS dust (2% of the dust mass) can give a significant contribution to the IRAS 25 micron flux. However, the presence of MgS in the detached shell cannot be inferred from the IRAS broadband photometry alone but requires infrared spectroscopy. We apply the model to the detached-shell sources R Scl and U Cam, both exhibiting a cool MgS feature in their ISO/SWS spectra. We use the shell parameters derived for the molecular shell, using the CO submillimetre maps. The models, with MgS grains located in the detached shell, explain the MgS grain temperature, as derived from their ISO spe...

  8. Hexuronic acid stereochemistry determination in chondroitin sulfate glycosaminoglycan oligosaccharides by electron detachment dissociation.

    Science.gov (United States)

    Leach, Franklin E; Ly, Mellisa; Laremore, Tatiana N; Wolff, Jeremy J; Perlow, Jacob; Linhardt, Robert J; Amster, I Jonathan

    2012-09-01

    Electron detachment dissociation (EDD) has previously provided stereo-specific product ions that allow for the assignment of the acidic C-5stereochemistry in heparan sulfate glycosaminoglycans (GAGs), but application of the same methodology to an epimer pair in the chondroitin sulfate glycoform class does not provide the same result. A series of experiments have been conducted in which glycosaminoglycan precursor ions are independently activated by electron detachment dissociation (EDD), electron induced dissociation (EID), and negative electron transfer dissociation (NETD) to assign the stereochemistry in chondroitin sulfate (CS) epimers and investigate the mechanisms for product ion formation during EDD in CS glycoforms. This approach allows for the assignment of electronic excitation products formed by EID and detachment products to radical pathways in NETD, both of which occur simultaneously during EDD. The uronic acid stereochemistry in electron detachment spectra produces intensity differences when assigned glycosidic and cross-ring cleavages are compared. The variations in the intensities of the doubly deprotonated (0,2)X(3) and Y(3) ions have been shown to be indicative of CS-A/DS composition during the CID of binary mixtures. These ions can provide insight into the uronic acid composition of binary mixtures in EDD, but the relative abundances, although reproducible, are low compared with those in a CID spectrum acquired on an ion trap. The application of principal component analysis (PCA) presents a multivariate approach to determining the uronic acid stereochemistry spectra of these GAGs by taking advantage of the reproducible peak distributions produced by electron detachment.

  9. Physiological and Molecular Changes of Detached Wheat Leaves in Responding to Various Treatments

    Institute of Scientific and Technical Information of China (English)

    Lifeng Zhao; Haoshan Zhang; Bingjing Zhang; Xiaojuan Bai; Chunjiang Zhou

    2012-01-01

    Leaf senescence is induced or accelerated when leaves are detached.However,the senescence process and expression pattern of senescence-associated genes (SAGs) when leaves are detached are not clearly understood.To detect senescence-associated physiological changes and SAG expression,wheat (Triticum aestivum L.) leaves were detached and treated with light,darkness,low temperature (4 ℃),jasmonic acid (JA),abscisic acid (ABA),and salicylic acid (SA).The leaf phenotypes,chlorophyll content,delayed fluorescence (DF),and expression levels of two SAGs,namely,TaSAG3 and TaSAG5,were analyzed.Under these different treatments,the detached leaves turned yellow with different patterns and varying chlorophyll content.DF significantly decreased after the dark,ABA,JA and SA treatments.TaSAG3 and TaSAG5,which are expressed in natural senescent leaves,showed different expression patterns under various treatments.However,both TaSAG3 and TaSAG5 were upregulated after leaf detachment.Our results revealed senescence-associated physiological changes and molecular differences in leaves,which induced leaf senescence during different stress treatments.

  10. The detached dust shells around the carbon AGB stars R Scl and V644 Sco

    CERN Document Server

    Maercker, Matthias; Leal-Ferreira, Marcelo M L; Olofsson, Göran; Florén, Hans-Gustav

    2014-01-01

    Detached shells are believed to be created during a thermal pulse, and constrain the time scales and physical properties of one of the main drivers of late stellar evolution. We aim at determining the morphology of the detached dust shells around the carbon AGB stars R Scl and V644 Sco, and compare this to observations of the detached gas shells. We observe the polarised, dust-scattered stellar light around these stars using the PolCor instrument mounted on the ESO 3.6m telescope. Observations were done with a coronographic mask to block out the direct stellar light. The polarised images clearly show the detached shells. Using a dust radiative transfer code to model the dust-scattered polarised light, we constrain the radii and widths of the shells to 19.5 arcsec and 9.4 arcsec for the detached dust shells around R Scl and V644 Sco, respectively. Both shells have an overall spherical symmetry and widths of approx. 2 arcsec. For R Scl we can compare the observed dust emission directly with high spatial-resolut...

  11. Intense-Field Multiple-Detachment of F2¯: Competition with Photodissociation.

    Science.gov (United States)

    Shahi, Abhishek; Albeck, Yishai; Strasser, Daniel

    2017-04-07

    The competition of intense-field multiple-detachment with efficient photodissociation of F2¯ is studied as a function of laser peak intensity. The main product channels are disentangled and characterized by 3D coincidence fragment imaging. The presented kinetic energy release spectra, angular distributions as well as two color pump-probe measurements allow identification of competing sequential and non-sequential mechanisms. Dissociative detachment, producing two neutral atoms (F + F) is found to be dominated by a sequential mechanism of photodissociation (F¯ + F) followed by detachment of the atomic anion fragment. In contrast, dissociative ionization (F + F(+)) shows competing contributions of both a sequential two-step mechanism as well as a non-sequential double-detachment of the molecular anion, which are distinguished by the kinetic energy released in the dissociation. Triple-detachment is found to be non-sequential in nature and results in Coulomb explosion (F(+)+F(+)). Furthermore, the measured kinetic energy release for dissociation on the (2)Σg(+) state provides a direct measurement of the F2¯ dissociation energy, D0 = 1.26±0.03 eV.

  12. Vitreous advanced glycation endproducts and α-dicarbonyls in retinal detachment patients with type 2 diabetes mellitus and non-diabetic controls

    Science.gov (United States)

    Mulder, Douwe J.; Schalkwijk, Casper G.; Scheijen, Jean L.; Smit, Andries J.; Los, Leonoor I.

    2017-01-01

    Purpose Advanced glycation endproducts (AGEs) and their precursors α-dicarbonyls are implicated in the progression of diabetic retinopathy. The purpose of this study was to assess AGEs and α-dicarbonyls in the vitreous of patients with type 2 diabetes mellitus (T2DM) with early stages or absence of diabetic retinopathy. Methods We examined vitreous samples obtained during vitrectomy from 31 T2DM patients presenting themselves with rhegmatogenous retinal detachment and compared these to 62 non-diabetic rhegmatogenous retinal detachment patients, matched on age, estimated glomerular filtration rate, smoking, intra-ocular lens implantation, and proliferative vitreoretinopathy. AGEs (pentosidine, Nε-(carboxymethyl)lysine, Nε-(carboxyethyl)lysine, and 5-hydro-5-methylimidazolone) and α-dicarbonyls (3-deoxyglucosone, methylglyoxal, and glyoxal) were measured by ultra performance liquid chromatography or high performance liquid chromatography. Skin autofluorescence was measured by the AGE Reader. Results Mean age was 64 ± 7.6 years for T2DM patients and 63 ± 8.1 years for controls. For T2DM patients, median diabetes duration was 2.2 (0.3–7.4) years. Non-proliferative diabetic retinopathy was present in 1 patient and classified as absent or background retinopathy in 30 patients. Vitreous levels of pentosidine (2.20 vs. 1.59 μmol/mol lysine, p = 0.012) and 3-deoxyglucosone (809 vs. 615 nmol/L, p = 0.001) were significantly elevated in T2DM patients compared to controls. Other AGEs and α-dicarbonyls in the vitreous were not significantly different. There was a trend for increased skin autofluorescence in T2DM patients as compared to controls (p = 0.07). Conclusions Pentosidine and 3-deoxyglucosone concentrations were increased in the vitreous of rhegmatogenous retinal detachment patients with a relatively short duration of diabetes compared to non-diabetic rhegmatogenous retinal detachment patients. PMID:28264049

  13. Effect of harvesting with a trunk shaker and an abscission chemical on fruit detachment and defoliation of citrus grown under Mediterranean conditions

    Energy Technology Data Exchange (ETDEWEB)

    Moreno, R.; Torregrosa, A.; Moltó, E.; Chueca, P.

    2015-07-01

    Spain ranks as the world’s leading exporter of citrus for fresh consumption. Manual harvest accounts for 50% of the total production costs. Mechanical harvest would increase labor productivity and benefits of growers. Efficiency of these machines depends on the varieties and operating conditions. Use of abscission chemicals has been promoted to increase the detachment rate of fruit without affecting its quality. This work is aimed at studying whether the mechanical harvest and/or the application of an abscission agent affect the quality and quantity of harvested fruit and tree defoliation under the conditions of citrus cultivation in Spain. Trials were made in a completely randomized experimental design. From 2008 to 2011, different orchards of mandarin and orange trees were sprayed with different doses of ethephon as abscission agent and harvested with a trunk shaker. Harvest related variables (detachment percentage, defoliation and fruit without calyx) were measured. The percentage of fruit detached by the trunk shaker ranged between 70 and 85% and it did not depend on the orchard. The shaker produced minimal damage to the bark when gripped incorrectly. Increased doses of ethephon increased fruit detachment except in ‘Clemenules’ orchard, but also increased the fruit without calyx in 1-9%. Moreover, ethephon promoted significant defoliation. Neither gummosis nor death of branches was observed. This work demonstrates that mechanical harvesting with trunk shakers may be a feasible solution for citrus cultivated in Spain for fresh market. Use of ethephon could only be recommended for citrus destined to industry and only for certain varieties. (Author)

  14. Effect of harvesting with a trunk shaker and an abscission chemical on fruit detachment and defoliation of citrus grown under Mediterranean conditions

    Directory of Open Access Journals (Sweden)

    Rosana Moreno

    2015-03-01

    Full Text Available Spain ranks as the world’s leading exporter of citrus for fresh consumption. Manual harvest accounts for 50% of the total production costs. Mechanical harvest would increase labor productivity and benefits of growers. Efficiency of these machines depends on the varieties and operating conditions. Use of abscission chemicals has been promoted to increase the detachment rate of fruit without affecting its quality. This work is aimed at studying whether the mechanical harvest and/or the application of an abscission agent affect the quality and quantity of harvested fruit and tree defoliation under the conditions of citrus cultivation in Spain. Trials were made in a completely randomized experimental design. From 2008 to 2011, different orchards of mandarin and orange trees were sprayed with different doses of ethephon as abscission agent and harvested with a trunk shaker. Harvest related variables (detachment percentage, defoliation and fruit without calyx were measured. The percentage of fruit detached by the trunk shaker ranged between 70 and 85% and it did not depend on the orchard. The shaker produced minimal damage to the bark when gripped incorrectly. Increased doses of ethephon increased fruit detachment except in ‘Clemenules’ orchard, but also increased the fruit without calyx in 1-9%. Moreover, ethephon promoted significant defoliation. Neither gummosis nor death of branches was observed. This work demonstrates that mechanical harvesting with trunk shakers may be a feasible solution for citrus cultivated in Spain for fresh market. Use of ethephon could only be recommended for citrus destined to industry and only for certain varieties.

  15. Psychological detachment from work during non-work time: linear or curvilinear relations with mental health and work engagement?

    Science.gov (United States)

    Shimazu, Akihito; Matsudaira, Ko; Jonge, Jan DE; Tosaka, Naoya; Watanabe, Kazuhiro; Takahashi, Masaya

    2016-06-10

    This study examined whether a higher level of psychological detachment during non-work time is associated with better employee mental health (Hypothesis 1), and examined whether psychological detachment has a curvilinear relation (inverted U-shaped pattern) with work engagement (Hypothesis 2). A large cross-sectional Internet survey was conducted among registered monitors of an Internet survey company in Japan. The questionnaire included scales for psychological detachment, employee mental health, and work engagement as well as for job characteristics and demographic variables as potential confounders. The hypothesized model was tested with moderated structural equation modeling techniques among 2,234 respondents working in the tertiary industries with regular employment. Results showed that psychological detachment had curvilinear relations with mental health as well as with work engagement. Mental health improved when psychological detachment increased from a low to higher levels but did not benefit any further from extremely high levels of psychological detachment. Work engagement showed the highest level at an intermediate level of detachment (inverted U-shaped pattern). Although high psychological detachment may enhance employee mental health, moderate levels of psychological detachment are most beneficial for his or her work engagement.

  16. Biotechnological applications of bacterial cellulases

    Directory of Open Access Journals (Sweden)

    Esther Menendez

    2015-08-01

    Full Text Available Cellulases have numerous applications in several industries, including biofuel production, food and feed industry, brewing, pulp and paper, textile, laundry, and agriculture.Cellulose-degrading bacteria are widely spread in nature, being isolated from quite different environments. Cellulose degradation is the result of a synergic process between an endoglucanase, an exoglucanase and a,β-glucosidase. Bacterial endoglucanases degrade ß-1,4-glucan linkages of cellulose amorphous zones, meanwhile exoglucanases cleave the remaining oligosaccharide chains, originating cellobiose, which is hydrolyzed by ß-glucanases. Bacterial cellulases (EC 3.2.1.4 are comprised in fourteen Glycosil Hydrolase families. Several advantages, such as higher growth rates and genetic versatility, emphasize the suitability and advantages of bacterial cellulases over other sources for this group of enzymes. This review summarizes the main known cellulolytic bacteria and the best strategies to optimize their cellulase production, focusing on endoglucanases, as well as it reviews the main biotechnological applications of bacterial cellulases in several industries, medicine and agriculture.

  17. Comparison of outcomes: scleral buckling and pars plana vitrectomy versus vitrectomy alone for primary repair of rhegmatogenous retinal detachment

    Directory of Open Access Journals (Sweden)

    Lindsell LB

    2016-12-01

    Full Text Available Luke B Lindsell, Robert A Sisk, Daniel M Miller, Robert E Foster, Michael R Petersen, Christopher D Riemann, Robert K Hutchins Cincinnati Eye Institute, Department of Ophthalmology, University of Cincinnati, Cincinnati, OH, USA Objective: To assess the combination of scleral buckling (SB and pars plana vitrectomy (PPV versus PPV alone in the primary repair of rhegmatogenous retinal detachments (RRDs.Methods: The current study was a retrospective, comparative, interventional, consecutive case series of 179 eyes of 174 patients who underwent primary RRD repair by five surgeons between January 1, 2008 and December 31, 2010, utilizing SB with PPV or PPV. Univariate and multivariate analyses were used to compare the efficacy of the two surgical strategies and assess for risk factors of proliferative vitreoretinopathy (PVR.Results: Single surgery anatomic success (SSAS was similar (P=0.76 between the PPV group (112 of 132 eyes, 85% and SB with PPV group (39 of 47 eyes, 83%. Final anatomic success was 100% in each group. There was no difference in rates of PVR formation (PPV 16% vs SB with PPV 19%, P=0.70. Final logarithm of the minimum angle of resolution acuity was 0.33 (20/43 in the PPV group and 0.37 (20/47 in the SB with PPV group (P=0.62. Postoperative anterior chamber fibrin was highly correlated with PVR formation (PVR 13% vs no PVR 0.7%, P=0.003; odds ratio =68.37, P=0.007. Separate analysis of medium- to high-complexity cases showed similar SSAS (PPV 86% vs SB with PPV 83%, P=0.45.Conclusion: SB with PPV versus PPV alone were similarly efficacious for repair of primary RRDs of varying complexity. SSAS rates, PVR incidence, and final visual acuities were not significantly different. Keywords: scleral buckling, vitrectomy, rhegmatogenous retinal detachment, outcomes, proliferative vitreoretinopathy, comparison

  18. The radontechnical process of the sub-slab suction system in detached, semi-detached and terraced houses; Imupistejaerjestelmaen radontekninen toiminta eri tyyppisissae pien- ja rivitaloissa

    Energy Technology Data Exchange (ETDEWEB)

    Kettunen, A.V.; Rissanen, R.; Viljanen, M. [Helsinki Univ. of Technology, Otaniemi (Finland). Lab. of Structural Engineering and Building Physics; Arvela, H. [Finnish Centre for Radiation and Nuclear Safety, Helsinki (Finland)

    1997-12-31

    The sub-slab suction system is a repair method for decreasing radon transfer from soil to indoor air in houses. The sub-slab suction system changes the flow field with the aim to reduce the radon content under the ground slab or/and changes the flow direction from indoor air to foundation soil. The study is divided into the theoretical and experimental part. The theoretical part concentrates on solving the factors affecting the sub-slab suction system taking into consideration features of old houses. The experimental part uses the results of the theoretical part when the level and the type of the suction point had been chosen. The effect of the sub-slab suction system was tested with pressure difference experiments and the radon content measurements in 26 different, typical Finnish detached, semi-detached and terrace houses.

  19. The Role of Cognitive Control in Older Adult Cognitive Reappraisal: Detached and Positive Reappraisal.

    Science.gov (United States)

    Liang, Ying; Huo, Meng; Kennison, Robert; Zhou, Renlai

    2017-01-01

    Older adults are more likely to regulate their emotions by engaging in cognitive reappraisal. However, depending on the type of cognitive reappraisal used, efforts to regulate emotions are sometimes met with success and other times with failure. It has been suggested the well-known age-related decline in cognitive control might be the culprit behind the poor use of detached reappraisal by older adults. However, this possibility has not been thoroughly investigated. In addition, studies have not examined what aspects of cognitive control- shifting, updating or inhibition-might be relevant to cognitive reappraisal. In the present study, 41 older participants were tested on cognitive control and abilities to use detached and positive reappraisal. Results showed detached reappraisal compared to positive relied more heavily on cognitive control, specifically mental set shifting. Results of this study have important implications for development of cognitive training interventions for older adults.

  20. Detached-eddy simulation of flow around the NREL phase VI blade

    DEFF Research Database (Denmark)

    Johansen, Jeppe; Sørensen, Niels N.; Michelsen, J.A.;

    2002-01-01

    –Stokes method in the boundary layer with a large-eddy simulation in the free shear flow. The present study focuses on static and dynamic stall regions highly relevant for stall-regulated wind turbines. Computations do predict force coefficients and pressure distributions fairly well. Results using detached-eddy......The detached-eddy simulation model implemented in the computational fluid dynamics code EllipSys3D is used to calculate the flow around the non-rotating NREL Phase VI wind turbine blade. Results are presented for flow around a parked blade at fixed angle of attack and a blade pitching along...... the blade axis. Computed blade characteristics are compared with experimental data from the NREL/NASA Ames Phase VI unsteady experiment. The detached-eddy simulation model is a method for predicting turbulence in computational fluid dynamics computations, which combines a Reynolds-averaged Navier...

  1. Ancient Wall Tiles – The Importance of the Glaze/Ceramic Interface in Glaze Detachment

    Directory of Open Access Journals (Sweden)

    Marisa COSTA

    2014-04-01

    Full Text Available One of the most severe pathologies suffered by early industrially produced tiles in Portugal in late nineteenth century is glaze detachment in wall tiles placed in the lower part of the façade. It is known that salts crystallize provoking the glaze detachment, destroying the waterproofing and the beauty of the wall tile and this is one of the crucial factors towards this occurrence. The present work questions the importance of the thickness of glaze/ceramic body interface, in what concerns glaze detachment provoked by salt crystallization. SEM-EDS was used to perform all the observations that lead to the conclusion that the exuberance of the interface between glaze and ceramic body has no influence in the resistance of the glaze to salt crystallization though time, being the porous network more determinant. DOI: http://dx.doi.org/10.5755/j01.ms.20.1.3815

  2. [Serous macular detachment associated with circumscribed choroidal hemangioma: a case report].

    Science.gov (United States)

    Munteanu, M; Giuri, Stela; Rosca, C; Boruga, O; Chercota, V; Stanca, H T

    2013-01-01

    Choroidal hemangiomas are benign vascular hamartomas that typically present from second to fourth decade of life, when they can cause visual disturbance due to exudative retinal detachment. They represent uncommon benign choroidal vascular tumors, usually occuring sporadically in the absence of systemic disease. We report the case of a young female patient, presenting a juxtapapillary circumscribed choroidal haemangioma complicated with serous macular detachment. The patient underwent photodynamic therapy with verteporfin (PDT). Systematic follow-up using ophthalmoscopy, ultrasonography, Fluorescein angiography and Indocyanine green angiography was performed. The patient presented regression with flattening of tumour, resolution of the subretinal fluid, and significant improvement of vision. Mostly asymptomatic, the choroidal hemangiomas can be associated with serous retinal detachment, leading to reduced vision and metamorphopsia; in those cases, the long term visual prognosis is poor, even in adequately treated patients. PDT is effective in eliminating the subretinal fluid and improving vision in patients with circumscribed choroidal hemangioma.

  3. Morphological Findings of Intraocular Lens with Haptics Detached from Optics Following Cataract Surgery.

    Science.gov (United States)

    Suzuki, Yukihiko; Suzuki, Kaori; Metok, Tomomi; Nakazawai, Mitsuru

    2010-03-09

    A 55-year-old woman was treated using phacoemulsification, aspiration, and intraocular lens (IOL) implantation. Three months after surgery, the optical zone of the anterior capsule was completely closed due to strong capsular contractions. As the two haptics were found to be completely detached from the optics, the IOL was subsequently extracted and morphologically examined. Although the optics and haptics of the IOL when viewed with a stereomicroscope appeared to have no marked damage on their surfaces, an ultra-structural examination showed that a portion of the acrylic optics on the detached surface was defective. We considered that the IOL haptics might have become detached from the optics due to continuous force from postoperative capsular contractions.

  4. The role of energy advisors on adoption of energy efficiency measures in detached houses

    Energy Technology Data Exchange (ETDEWEB)

    Mahapatra, Krushna; Nair, Gireesh; Gustavsson, Leif (Ecotechnology, Mid Sweden Univ., Oestersund (Sweden)). e-mail: leif.gustavsson@miun.se

    2009-07-01

    External actors can influence potential adopters to adopt energy efficiency measures. In Sweden energy advisers are one such actor group who provides energy advice and information to the end users. Currently, all municipalities offer energy advisers' service. The success of such service for improvement of energy efficiency of detached houses depends on homeowners' perception towards it. In this context we conducted a national survey of about 3000 owners of detached houses through stratified random sampling method in 2008 summer. We found that majority of owners' of detached houses consider energy advisers as an important source of information. Furthermore, many homeowners who contacted energy advisers for advice had implemented the suggestions. However, only a few homeowners had actually contacted energy advisers. Our findings suggest that it is beneficial to continue the energy adviser service, but more efforts are needed to increase homeowners' awareness of and satisfaction with such services.

  5. Single-electron detachment cross sections in H{sup {minus}}+Ar

    Energy Technology Data Exchange (ETDEWEB)

    Kvale, T.J.; Sen, A.; Matulioniene, A.S.R.; Fang, X. [Univ. of Toledo, OH (United States)

    1993-05-01

    Measurements of absolute cross sections for the single electron detachment ({sigma}{sub -1,0}) in collisions between H{sup -} and Ar in the energy range 5-50 keV are reported. A well-collimated H{sup -} ion beam is directed toward an Ar gas target and all three of the exiting scattered beam components (i.e. H{sup 0}, H{sup +} and H{sup -}) are measured simultaneously. Using the closure relation among the collision beam components, (H{sup 0}, H{sup -} and H{sup +}) the neutral detection efficiencies are determined at each energy and thereby we are able to report absolute single detachment cross sections. The single detachment cross sections {sigma}{sub -1,0} exhibit a broad maximum with a value of 1.78 x 10{sup -15} cm{sup 2} at an impact energy of 25 keV.

  6. A path-independent integral for the characterization of solute concentration and flux at biofilm detachments

    Science.gov (United States)

    Moran, B.; Kulkarni, S.S.; Reeves, H.W.

    2007-01-01

    A path-independent (conservation) integral is developed for the characterization of solute concentration and flux in a biofilm in the vicinity of a detachment or other flux limiting boundary condition. Steady state conditions of solute diffusion are considered and biofilm kinetics are described by an uptake term which can be expressed in terms of a potential (Michaelis-Menten kinetics). An asymptotic solution for solute concentration at the tip of the detachment is obtained and shown to be analogous to that of antiplane crack problems in linear elasticity. It is shown that the amplitude of the asymptotic solution can be calculated by evaluating a path-independent integral. The special case of a semi-infinite detachment in an infinite strip is considered and the amplitude of the asymptotic field is related to the boundary conditions and problem parameters in closed form for zeroth and first order kinetics and numerically for Michaelis-Menten kinetics. ?? Springer Science+Business Media, Inc. 2007.

  7. Modeling of particle layer detachment-Role of transient kinetic effects

    Institute of Scientific and Technical Information of China (English)

    Qian Zhang; Eberhard Schmidt

    2009-01-01

    Particle layers tend to build up on walls in many filtration and separation processes, calling for periodic removal in order to keep the apparatus running. Important factors are the adhesion of the layer on the substrate and the cohesion of the particles in the layer. Models describing such layer detachment generally assume constant and homogeneous conditions for the forces acting on the layer. But in reality detachment is extremely non-stationary concerning place and time, primarily due to changing conditions of the detaching forces on the one hand and changes in the particle layer morphology on the other. This paper describes a model and a simulation based on this model considering such transient kinetic effects, for which some computing results are presented and discussed.

  8. Two cases of exudative retina detachment and uveitis following H1N1 influenza vaccination

    Institute of Scientific and Technical Information of China (English)

    TAO Yong; CHANG Li-bing; ZHAO Min; LI Xiao-xin

    2011-01-01

    Uveitis was a rare adverse event of vaccination.We met two cases of acute uveitis with exudative retinal detachment following vaccination of H1N1 influenza.Case 1 was a 10-year-old boy who was admitted for bilateral blurred vision at 10 days after vaccination of H1N1 influenza.Vitreous opacity was obvious in both eyes.Broad exudative retinal detachment was observed in the right eye.Case 2 was a 47-year-old female who suffered from an acute high fever at 2 days after the vaccination of H1 N1 influenza.Later,she encountered bilateral headache and decreasing vision.In both eyes,mutton fat keratic precipitates,positive Tyndall phenomenon,congestion of optic disc and exudative retinal detachment were observed.

  9. Influence of plasma resistance and fluctuation on probe characteristics in detached recombining plasmas

    Energy Technology Data Exchange (ETDEWEB)

    Ohno, N.; Tanaka, N.; Nishijima, D.; Takamura, S. [Nagoya Univ. (Japan). Dept. of Energy Engineering and Science; Ezumi, N. [Dept. of Electronics and Control Engineering, Nagano National Coll. of Technology, Tokuma (Japan)

    2001-07-01

    In order to find the causes of the strong anomaly of current-voltage characteristics of Langmuir probe observed in detached recombining plasmas in a linear divertor plasma simulator, NAGDIS-II, we have investigated plasma resistance along a magnetic field and potential fluctuations in the detached recombining plasmas. Simple calculation on the ratio between the plasma length, at which plasma resistance and resistance of ion sheath formed around a probe tip become equal, and an electron collection length indicates that the evaluation of electron temperature T{sub e} becomes inaccurate at T{sub e} of less than 0.6 eV when plasma density and neutral pressure are 1.0 x 10{sup 18} m{sup -3} and 10 mtorr, respectively. The potential fluctuation in detached recombining plasmas was found to be so large compared to T{sub e}/e, which can also modify the probe characteristics. (orig.)

  10. Postoperative Recovery of Light Sensitivity in Eyes with Rhegmatogenous Retinal Detachment.

    Science.gov (United States)

    Ooshiro, Tomohiro; Iijima, Hiroyuki

    2017-01-01

    To investigate whether postoperative light sensitivity recovers completely to the level prior to the development of rhegmatogenous retinal detachment (RRD) after successful surgery. We retrospectively studied 44 eyes of 44 patients with RRD who were successfully operated and who underwent Humphrey central 30-2 perimetry postoperatively. The averaged total deviation in Humphrey perimetry in the reattached retina was compared with that of the horizontal or vertical counterpart in the preoperatively non-detached retina. The averaged total deviation in the reattached retina was significantly lower than in its counterpart (p sensitivity did not correlate with postoperative visual acuity (p = 0.8047) or with its change (p = 0.1242). Light sensitivity in the detached retina in eyes with RRD does not completely recover after successful surgery. © 2017 The Author(s) Published by S. Karger AG, Basel.

  11. Management of retinal detachment in block related globe perforation with pneumatic retinopexy.

    Science.gov (United States)

    Rishi, Karandeep; Venkatesh, Pradeep; Garg, Satpal P

    2013-03-01

    Retinal detachment after ocular perforation related to local anesthesia is a common complication, which is usually associated with a poor prognosis despite complex vitreoretinal surgical procedures. We report a case of 62-year-old male with cataract surgery done 4 weeks back with nasal retinal detachment with a posterior break. Pneumatic retinopexy was performed and laser barrage of the breaks was done the next day when the retina got attached. A vision of 20/30 was achieved at the end of 2 months. To the best of our knowledge, this is a first case report in literature where pneumatic retinopexy was used to manage a retinal detachment caused by block-related perforation.

  12. Management of retinal detachment in block related globe perforation with pneumatic retinopexy

    Directory of Open Access Journals (Sweden)

    Karandeep Rishi

    2013-01-01

    Full Text Available Retinal detachment after ocular perforation related to local anesthesia is a common complication, which is usually associated with a poor prognosis despite complex vitreoretinal surgical procedures. We report a case of 62-year-old male with cataract surgery done 4 weeks back with nasal retinal detachment with a posterior break. Pneumatic retinopexy was performed and laser barrage of the breaks was done the next day when the retina got attached. A vision of 20/30 was achieved at the end of 2 months. To the best of our knowledge, this is a first case report in literature where pneumatic retinopexy was used to manage a retinal detachment caused by block-related perforation.

  13. Photo excitation and laser detachment of C60 − anions in a storage ring

    DEFF Research Database (Denmark)

    Støchkel, Kristian; Andersen, Jens Ulrik

    2013-01-01

    (REMPED) has been repeated both at room temperature and with the trap cooled to liquid nitrogen temperature. However, wavelength dependence of the overlap of the strongly focused laser beam with the ion beam introduces distortions of the absorption spectrum. We have therefore applied a new method...... level, is much weaker in the new measurements and could be an H g vibrational sideband. Also earlier studies of direct laser detachment from C60 − in the storage ring ASTRID have been revisited, with ions cooled by liquid nitrogen in the ion trap. We confirm the previous measurement with a determination......We have studied the photo physics of C60 − anions in the electrostatic storage ring ELISA with ions produced in a plasma source and cooled and bunched in a He filled ion trap. A previous study using delayed electron detachment as a signal of resonance-enhanced multiphoton electron detachment...

  14. Farallon slab detachment and deformation of the Magdalena Shelf, southern Baja California

    Science.gov (United States)

    Brothers, Daniel S.; Harding, Alistair J.; Gonzalez-Fernandez, Antonio; Holbrook, W.S. Steven; Kent, Graham M.; Driscoll, Neal W.; Fletcher, John M.; Lizarralde, Daniel; Umhoefer, Paul J.; Axen, Gary

    2012-01-01

    Subduction of the Farallon plate beneath northwestern Mexico stalled by ~12 Ma when the Pacific-Farallon spreading-ridge approached the subduction zone. Coupling between remnant slab and the overriding North American plate played an important role in the capture of the Baja California (BC) microplate by the Pacific Plate. Active-source seismic reflection and wide-angle seismic refraction profiles across southwestern BC (~24.5°N) are used to image the extent of remnant slab and study its impact on the overriding plate. We infer that the hot, buoyant slab detached ~40 km landward of the fossil trench. Isostatic rebound following slab detachment uplifted the margin and exposed the Magdalena Shelf to wave-base erosion. Subsequent cooling, subsidence and transtensional opening along the shelf (starting ~8 Ma) starved the fossil trench of terrigenous sediment input. Slab detachment and the resultant rebound of the margin provide a mechanism for rapid uplift and exhumation of forearc subduction complexes.

  15. Tunable wavefront coded imaging system based on detachable phase mask: Mathematical analysis, optimization and underlying applications

    Science.gov (United States)

    Zhao, Hui; Wei, Jingxuan

    2014-09-01

    The key to the concept of tunable wavefront coding lies in detachable phase masks. Ojeda-Castaneda et al. (Progress in Electronics Research Symposium Proceedings, Cambridge, USA, July 5-8, 2010) described a typical design in which two components with cosinusoidal phase variation operate together to make defocus sensitivity tunable. The present study proposes an improved design and makes three contributions: (1) A mathematical derivation based on the stationary phase method explains why the detachable phase mask of Ojeda-Castaneda et al. tunes the defocus sensitivity. (2) The mathematical derivations show that the effective bandwidth wavefront coded imaging system is also tunable by making each component of the detachable phase mask move asymmetrically. An improved Fisher information-based optimization procedure was also designed to ascertain the optimal mask parameters corresponding to specific bandwidth. (3) Possible applications of the tunable bandwidth are demonstrated by simulated imaging.

  16. Evolution of Bacterial Suicide

    Science.gov (United States)

    Tchernookov, Martin; Nemenman, Ilya

    2013-03-01

    While active, controlled cellular suicide (autolysis) in bacteria is commonly observed, it has been hard to argue that autolysis can be beneficial to an individual who commits it. We propose a theoretical model that predicts that bacterial autolysis is evolutionarily advantageous to an individualand would fixate in physically structured environments for stationary phase colonies. We perform spatially resolved agent-based simulations of the model, which predict that lower mixing in the environment results in fixation of a higher autolysis rate from a single mutated cell, regardless of the colony's genetic diversity. We argue that quorum sensing will fixate as well, even if initially rare, if it is coupled to controlling the autolysis rate. The model does not predict a strong additional competitive advantage for cells where autolysis is controlled by quorum sensing systems that distinguish self from nonself. These predictions are broadly supported by recent experimental results in B. subtilisand S. pneumoniae. Research partially supported by the James S McDonnell Foundation grant No. 220020321 and by HFSP grant No. RGY0084/2011.

  17. What Are the Origins of Detached Layers of Dust on Mars ? Investigation with Global Climate Model

    Science.gov (United States)

    Bertrand, T.; Spiga, A.; Forget, F.

    2014-12-01

    The climate on Mars is strongly controlled by the amount of dust lifted and transported in the atmosphere, which causes fluctuations of air opacity and affects temperatures and winds. Recently, observations of the vertical dust distribution of the Martian atmosphere by the Mars Climate Sounder on board the Mars Reconnaissance Orbiter revealed a phenomenon which is still poorly understood: the formation of detached layers of dust. These detached layers, also confirmed by the Thermal Emission Spectrometer on-board the Mars Global Surveyor, reside above the planetary boundary layer typically at altitudes between 20 and 40 km and have been mostly observed at low latitudes. These detached layers of dust are not reproduced by Global Climate Models (GCM) and different atmospheric processes are discussed and can be combined to explain their origin, such as small-scale lifting, upslope topographic winds, scavenging by water ice clouds, dust storms… Here we use the Martian GCM developed at the Laboratoire de Météorologie Dynamique (LMD) to simulate the formation of detached layers of dust. To start, we developed a new implementation of the water cycle, taking into account nucleation on dust particles, ice particle growth, and scavenging of dust particles due to the condensation of ice. However, this method didn't yield to satisfying results in the GCM. Then, we performed the parameterization in the GCM of the so-called "rocket dust storms", governed by deep convection and able to inject dust at high altitudes in the Martian troposphere. By coupling this new parameterization with general circulation of the GCM, we succeed to model detached layers of dust. Here we present this parameterization and we discuss about the spatial and temporal variability of the detached layers of dust, in comparison with observations.

  18. Keratinocyte detachment-differentiation connection revisited, or anoikis-pityriasi nexus redux.

    Directory of Open Access Journals (Sweden)

    Tomohiro Banno

    Full Text Available Epidermis, a continuously self-renewing and differentiating organ, produces a protective stratum corneum that shields us from external chemical, physical and microbial threats. Epidermal differentiation is a multi-step process regulated by influences, some unknown, others insufficiently explored. Detachment of keratinocytes from the basement membrane is one such pro-differentiation stimulus. Here, we define the transcriptional changes during differentiation, especially those caused by detachment from the substratum. Using comprehensive transcriptional profiling, we revisited the effects of detachment as a differentiation signal to keratinocytes. We identified the genes regulated by detachment, the corresponding ontological categories and, using metaanalysis, compared the genes and categories to those regulated by other pro-differentiating stimuli. We identified 762 genes overexpressed in suspended keratinocyte, including known and novel differentiation markers, and 1427 in attached cells, including basal layer markers. Detachment induced epidermis development, cornification and desmosomal genes, but also innate immunity, proliferation inhibitors, transcription regulators and MAPKs; conversely the attached cells overexpressed cell cycle, anchoring, motility, splicing and mitochondrial genes, and both positive and negative regulators of apoptosis. Metaanalysis identified which detachment-regulated categories overlap with those induced by suprabasal location in vivo, by reaching confluency in vitro, and by inhibition of JUN kinases. Attached and in vivo basal cells shared overexpression of mitochondrial components. Interestingly, melanosome trafficking components were also overexpressed in the attached and in vivo basal keratinocytes. These results suggest that specific pro-differentiation signals induce specific features of the keratinization process, which are in vivo orchestrated into harmonious epidermal homeostasis.

  19. Detachment of deposited colloids by advancing and receding air-water interfaces.

    Science.gov (United States)

    Aramrak, Surachet; Flury, Markus; Harsh, James B

    2011-08-16

    Moving air-water interfaces can detach colloidal particles from stationary surfaces. The objective of this study was to quantify the effects of advancing and receding air-water interfaces on colloid detachment as a function of interface velocity. We deposited fluorescent, negatively charged, carboxylate-modified polystyrene colloids (diameter of 1 μm) into a cylindrical glass channel. The colloids were hydrophilic with an advancing air-water contact angle of 60° and a receding contact angle of 40°. After colloid deposition, two air bubbles were sequentially introduced into the glass channel and passed through the channel at different velocities (0.5, 7.7, 72, 982, and 10,800 cm/h). The passage of the bubbles represented a sequence of receding and advancing air-water interfaces. Colloids remaining in the glass channel after each interface passage were visualized with confocal microscopy and quantified by image analysis. The advancing air-water interface was significantly more effective in detaching colloids from the glass surface than the receding interface. Most of the colloids were detached during the first passage of the advancing air-water interface, while the subsequent interface passages did not remove significant amounts of colloids. Forces acting on the colloids calculated from theory corroborate our experimental results, and confirm that the detachment forces (surface tension forces) during the advancing air-water interface movement were stronger than during the receding movement. Theory indicates that, for hydrophilic colloids, the advancing interface movement generally exerts a stronger detachment force than the receding, except when the hysteresis of the colloid-air-water contact angle is small and that of the channel-air-water contact angle is large.

  20. Transcriptomic analysis of human retinal detachment reveals both inflammatory response and photoreceptor death.

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    Marie-Noëlle Delyfer

    Full Text Available BACKGROUND: Retinal detachment often leads to a severe and permanent loss of vision and its therapeutic management remains to this day exclusively surgical. We have used surgical specimens to perform a differential analysis of the transcriptome of human retinal tissues following detachment in order to identify new potential pharmacological targets that could be used in combination with surgery to further improve final outcome. METHODOLOGY/PRINCIPAL FINDINGS: Statistical analysis reveals major involvement of the immune response in the disease. Interestingly, using a novel approach relying on coordinated expression, the interindividual variation was monitored to unravel a second crucial aspect of the pathological process: the death of photoreceptor cells. Within the genes identified, the expression of the major histocompatibility complex I gene HLA-C enables diagnosis of the disease, while PKD2L1 and SLCO4A1 -which are both down-regulated- act synergistically to provide an estimate of the duration of the retinal detachment process. Our analysis thus reveals the two complementary cellular and molecular aspects linked to retinal detachment: an immune response and the degeneration of photoreceptor cells. We also reveal that the human specimens have a higher clinical value as compared to artificial models that point to IL6 and oxidative stress, not implicated in the surgical specimens studied here. CONCLUSIONS/SIGNIFICANCE: This systematic analysis confirmed the occurrence of both neurodegeneration and inflammation during retinal detachment, and further identifies precisely the modification of expression of the different genes implicated in these two phenomena. Our data henceforth give a new insight into the disease process and provide a rationale for therapeutic strategies aimed at limiting inflammation and photoreceptor damage associated with retinal detachment and, in turn, improving visual prognosis after retinal surgery.