Morphology and Chemical Composition of soot particles emitted by Wood-burning Cook-Stoves: a HRTEM, XPS and Elastic backscattering Studies.

Science.gov (United States)

Carabali-Sandoval, G. A., Sr.; Castro, T.; Peralta, O.; De la Cruz, W.; Días, J.; Amelines, O.; Rivera-Hernández, M.; Varela, A.; Muñoz-Muñoz, F.; Policroniades, R.; Murillo, G.; Moreno, E.

2014-12-01

The morphology, microstructure and the chemical composition on surface of soot particles were studied by using high resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS) and elastic backscattering spectrometry. In order to obtain freshly soot particles emitted by home-made wood-burning cook stoves, copper grids for Transmission Electron Microscope (TEM) were placed on the last two of an 8-stages MOUDI cascade impactor. The analysis of HRTEM micrographs revealed the nanostructure and the particle size of soot particles. The XPS survey spectra show a large carbon peak around 285 eV and the oxygen signal at 533 eV. Some differences observed in the carbon/oxygen (C/O) ratio of the particles probably depend on the combustion process efficiency of each cook-stove analyzed. The C-1s XPS spectra show an asymmetric broad peak and other with low intensity that corresponds to sp2 and sp3hybridization, which were fitted with a convolution using Gaussian functions. Elastic backscattering technique allows a chemical elemental analysis of samples and confirms the presence of C, O and Si observed by XPS. Additionally, the morphological properties of soot aggregates were analyzed calculating the border-based fractal dimension (Df). Particles exhibit complex shapes with high values of Df. Also, real-time absorption (σabs) and scattering (σsct) coefficients of fine (with aerodynamic diameter < 2.5 µm) soot particles were measured. The trend in σabs and σsct indicate that the cooking process has two important combustion stages which varied in its flaming strength, being vigorous in the first stage and soft in the second one.

Extracting electron backscattering coefficients from backscattered electron micrographs

International Nuclear Information System (INIS)

Zupanic, F.

2010-01-01

Electron backscattering micrographs possess the so-called Z-contrast, carrying information about the chemical compositions of phases present in microstructures. The intensity at a particular point in the backscattered electron micrograph is proportional to the signal detected at a corresponding point in the scan raster, which is, in turn, proportional to the electron backscattering coefficient of a phase at that point. This article introduces a simple method for extracting the electron backscattering coefficients of phases present in the microstructure, from the backscattered electron micrographs. This method is able to convert the micrograph's greyscale to the backscattering-coefficient-scale. The prerequisite involves the known backscattering coefficients for two phases in the micrograph. In this way, backscattering coefficients of other phases can be determined. The method is unable to determine the chemical compositions of phases or the presence of an element only from analysing the backscattered electron micrograph. Nevertheless, this method was found to be very powerful when combined with energy dispersive spectroscopy, and the calculations of backscattering coefficients. - Research Highlights: →A simple method for extracting the electron backscattering coefficients →The prerequisite is known backscattering coefficients for two phases →The information is complementary to the EDS-results. →This method is especially useful when a phase contains a light element (H, Li, Be, and B)

Microfluidic Platform for Enzyme-Linked and Magnetic Particle-Based Immunoassay

Directory of Open Access Journals (Sweden)

Dorota G. Pijanowska

2013-06-01

Full Text Available This article presents design and testing of a microfluidic platform for immunoassay. The method is based on sandwiched ELISA, whereby the primary antibody is immobilized on nitrocelluose and, subsequently, magnetic beads are used as a label to detect the analyte. The chip takes approximately 2 h and 15 min to complete the assay. A Hall Effect sensor using 0.35-μm BioMEMS TSMC technology (Taiwan Semiconductor Manufacturing Company Bio-Micro-Electro-Mechanical Systems was fabricated to sense the magnetic field from the beads. Furthermore, florescence detection and absorbance measurements from the chip demonstrate successful immunoassay on the chip. In addition, investigation also covers the Hall Effect simulations, mechanical modeling of the bead–protein complex, testing of the microfluidic platform with magnetic beads averaging 10 nm, and measurements with an inductor-based system.

Particle-in-cell studies of laser-driven hot spots and a statistical model for mesoscopic properties of Raman backscatter

International Nuclear Information System (INIS)

Albright, B.J.; Yin, L.; Bowers, K.J.; Kline, J.L.; Montgomery, D.S.; Fernandez, J.C.; Daughton, W.

2006-01-01

The authors use explicit particle-in-cell simulations to model stimulated scattering processes in media with both solitary and multiple laser hot spots. These simulations indicate coupling among hot spots, whereby scattered light, plasma waves, and hot electrons generated in one laser hot spot may propagate to neighboring hot spots, which can be destabilized to enhanced backscatter. A nonlinear statistical model of a stochastic beam exhibiting this coupled behavior is described here. Calibration of the model using particle-in-cell simulations is performed, and a threshold is derived for 'detonation' of the beam to high reflectivity. (authors)

Calculated effects of backscattering on skin dosimetry for nuclear fuel fragments

International Nuclear Information System (INIS)

Aydarous, A. Sh

2008-01-01

The size of hot particles contained in nuclear fallout ranges from 10 nm to 20 μm for the worldwide weapons fallout. Hot particles from nuclear power reactors can be significantly bigger (100 μm to several millimetres). Electron backscattering from such particles is a prominent secondary effect in beta dosimetry for radiological protection purposes, such as skin dosimetry. In this study, the effect of electron backscattering due to hot particles contamination on skin dose is investigated. These include parameters such as detector area, source radius, source energy, scattering material and source density. The Monte-Carlo Neutron Particle code (MCNP4C) was used to calculate the depth dose distribution for 10 different beta sources and various materials. The backscattering dose factors (BSDF) were then calculated. A significant dependence is shown for the BSDF magnitude upon detector area, source radius and scatterers. It is clearly shown that the BSDF increases with increasing detector area. For high Z scatterers, the BSDF can reach as high as 40 and 100% for sources with radii 0.1 and 0.0001 cm, respectively. The variation of BSDF with source radius, source energy and source density is discussed. (authors)

Improvements in backscatter measurement devices

International Nuclear Information System (INIS)

Saunders, J.; Hay, W.D.

1978-01-01

Improvements in measuring the thickness of a coating on a substrate by the technique of backscattered particles are described. These improvements enable the measurements to be carried out continuously as an integral part of the coating production line and also permit measurements where the coated elements are separated from one another by a predetermined distance. The former is achieved by situating the backscatter probe and detector on the rim of the measurement wheel and rotating this wheel at a speed such that the coated element and probe are stationary relative to one another. The latter improvement is achieved by an indexing apparatus which automatically positions the probe beside a coated element. (U.K.)

Comparison of STIM and particle backscattering spectrometry mass determination for quantitative microanalysis of cultured cells

International Nuclear Information System (INIS)

Deves, G.; Ortega, R.

2001-01-01

In biological sample microanalysis, a mass-normalisation method is commonly used as a quantitative index of elemental concentrations determined by particle-induced X-ray emission (PIXE). The organic mass can either be determined using particle backscattering spectrometry (BS) or scanning transmission ion microscopy (STIM). However, the accuracy of quantitative microanalysis in samples such as cultured cells is affected by beam-induced loss of organic mass during analysis. The aim of this paper is to compare mass measurements determined by particle BS or by STIM. In order to calibrate STIM and BS analyses, we measured by both techniques the thickness of standard foils of polycarbonate (3 and 6 μm), Mylar[reg] (4 μm), Kapton[reg] (7.5 μm) and Nylon[reg] (15 μm), as well as biological samples of mono-layered cultured cells. Non-damaging STIM analysis of samples before PIXE irradiation is certainly one of the most accurate ways to determine the sample mass, however, this requires strong experimental handling. On the other hand, BS performed simultaneously to PIXE is the simplest method to determine the local mass in polymer foils, but appears less accurate in the case of cultured cells

A Bidirectional Subsurface Remote Sensing Reflectance Model Explicitly Accounting for Particle Backscattering Shapes

Science.gov (United States)

He, Shuangyan; Zhang, Xiaodong; Xiong, Yuanheng; Gray, Deric

2017-11-01

The subsurface remote sensing reflectance (rrs, sr-1), particularly its bidirectional reflectance distribution function (BRDF), depends fundamentally on the angular shape of the volume scattering functions (VSFs, m-1 sr-1). Recent technological advancement has greatly expanded the collection, and the knowledge of natural variability, of the VSFs of oceanic particles. This allows us to test the Zaneveld's theoretical rrs model that explicitly accounts for particle VSF shapes. We parameterized the rrs model based on HydroLight simulations using 114 VSFs measured in three coastal waters around the United States and in oceanic waters of North Atlantic Ocean. With the absorption coefficient (a), backscattering coefficient (bb), and VSF shape as inputs, the parameterized model is able to predict rrs with a root mean square relative error of ˜4% for solar zenith angles from 0 to 75°, viewing zenith angles from 0 to 60°, and viewing azimuth angles from 0 to 180°. A test with the field data indicates the performance of our model, when using only a and bb as inputs and selecting the VSF shape using bb, is comparable to or slightly better than the currently used models by Morel et al. and Lee et al. Explicitly expressing VSF shapes in rrs modeling has great potential to further constrain the uncertainty in the ocean color studies as our knowledge on the VSFs of natural particles continues to improve. Our study represents a first effort in this direction.

Card controlled beta backscatter thickness measuring instrument

International Nuclear Information System (INIS)

Schlesinger, J.

1978-01-01

An improved beta backscatter instrument for the nondestructive measurement of the thickness of thin coatings on a substrate is described. Included therein is the utilization of a bank of memory stored data representative of isotope, substrate, coating material and thickness range characteristics in association with a control card having predetermined indicia thereon selectively representative of a particular isotope, substrate material, coating material and thickness range for conditioning electronic circuit means by memory stored data selected in accord with the predetermined indicia on a control card for converting backscattered beta particle counts into indicia of coating thickness

Magnetic Particle-Based Immunoassay of Phosphorylated p53 Using Protein-Cage Templated Lead Phosphate and Carbon Nanospheres for Signal Amplification

Energy Technology Data Exchange (ETDEWEB)

Chen, Aiqiong; Bao, Yuanwu; Ge, Xiaoxiao; Shin, Yongsoon; Du, Dan; Lin, Yuehe

2012-11-20

Phosphorylated p53 at serin 15 (phospho-p53-15) is a potential biomarker of Gamma-radiation exposure. In this paper, we described a new magnetic particles (MPs)-based electrochemical immunoassay of human phospho-p53-15 using carbon nanospheres (CNS) and protein-cage templated lead phosphate nanoparticles for signal amplification. Greatly enhanced sensitivity was achieved by three aspects: 1) The protein-cage nanoparticle (PCN) and p53-15 signal antibody (p53-15 Ab2) are linked to CNS (PCNof each apoferritin; 3) MPs capture a large amount of primary antibodies. Using apoferritin templated metallic phosphate instead of enzyme as label has the advantage of eliminating the addition of mediator or immunoreagents and thus makes the immunoassay system simpler. The subsequent stripping voltammetric analysis of the released lead ions were detected on a disposable screen printed electrode. The response current was proportional to the phospho-p53-15 concentration in the range of 0.02 to 20 ng mL-1 with detection limit of 0.01 ng mL-1. This method shows a good stability, reproducibility and recovery.

Particle backscatter and relative humidity measured across cirrus clouds and comparison with microphysical cirrus modelling

Directory of Open Access Journals (Sweden)

M. Brabec

2012-10-01

Full Text Available Advanced measurement and modelling techniques are employed to estimate the partitioning of atmospheric water between the gas phase and the condensed phase in and around cirrus clouds, and thus to identify in-cloud and out-of-cloud supersaturations with respect to ice. In November 2008 the newly developed balloon-borne backscatter sonde COBALD (Compact Optical Backscatter and AerosoL Detector was flown 14 times together with a CFH (Cryogenic Frost point Hygrometer from Lindenberg, Germany (52° N, 14° E. The case discussed here in detail shows two cirrus layers with in-cloud relative humidities with respect to ice between 50% and 130%. Global operational analysis data of ECMWF (roughly 1° × 1° horizontal and 1 km vertical resolution, 6-hourly stored fields fail to represent ice water contents and relative humidities. Conversely, regional COSMO-7 forecasts (6.6 km × 6.6 km, 5-min stored fields capture the measured humidities and cloud positions remarkably well. The main difference between ECMWF and COSMO data is the resolution of small-scale vertical features responsible for cirrus formation. Nevertheless, ice water contents in COSMO-7 are still off by factors 2–10, likely reflecting limitations in COSMO's ice phase bulk scheme. Significant improvements can be achieved by comprehensive size-resolved microphysical and optical modelling along backward trajectories based on COSMO-7 wind and temperature fields, which allow accurate computation of humidities, homogeneous ice nucleation, resulting ice particle size distributions and backscatter ratios at the COBALD wavelengths. However, only by superimposing small-scale temperature fluctuations, which remain unresolved by the numerical weather prediction models, can we obtain a satisfying agreement with the observations and reconcile the measured in-cloud non-equilibrium humidities with conventional ice cloud microphysics. Conversely, the model-data comparison provides no evidence that additional

A magnetic particles-based chemiluminescence enzyme immunoassay for rapid detection of ovalbumin.

Science.gov (United States)

Feng, Xiao-Li; Ren, Hong-Lin; Li, Yan-Song; Hu, Pan; Zhou, Yu; Liu, Zeng-Shan; Yan, Dong-Ming; Hui, Qi; Liu, Dong; Lin, Chao; Liu, Nan-Nan; Liu, Yan-Yan; Lu, Shi-Ying

2014-08-15

Egg allergy is an important public health and safety concern, so quantification and administration of food or vaccines containing ovalbumin (OVA) are urgently needed. This study aimed to establish a rapid and sensitive magnetic particles-chemiluminescence enzyme immunoassay (MPs-CLEIA) for the determination of OVA. The proposed method was developed on the basis of a double antibodies sandwich immunoreaction and luminol-H2O2 chemiluminescence system. The MPs served as both the solid phase and separator, the anti-OVA MPs-coated polyclonal antibodies (pAbs) were used as capturing antibody, and the horseradish peroxidase (HRP)-labeled monoclonal antibody (mAb) was taken as detecting antibody. The parameters of the method were evaluated and optimized. The established MPs-CLEIA method had a linear range from 0.31 to 100ng/ml with a detection limit of 0.24ng/ml. The assays showed low reactivities and less than 5% of intraassay and interassay coefficients of variation (CVs), and the average recoveries were between 92 and 97%. Furthermore, the developed method was applied in real samples analysis successfully, and the correlation coefficient with the commercially available OVA kit was 0.9976. Moreover, it was more rapid and sensitive compared with the other methods for testing OVA. Copyright © 2014 Elsevier Inc. All rights reserved.

Results of Monte-Carlo studies on backscattering and sputtering from 'pocket' and 'finned' structures

International Nuclear Information System (INIS)

Brown, K.P.

1978-01-01

A Monte-Carlo computer program which has been developed for studying backscattering and sputtering processes involving high energy particles in complex vacuum structures has been used to show that useful reductions in backscattering and sputtering can be achieved by pocketing or finning the wall surfaces of plasma containment vessels. (author)

Multiple scattering wavelength dependent backscattering of kaolin dust in the IR: Measurements and theory

Science.gov (United States)

Ben-David, Avishai

1992-01-01

Knowing the optical properties of aerosol dust is important for designing electro-optical systems and for modeling the effect on propagation of light in the atmosphere. As CO2 lidar technology becomes more advanced and is used for multiwavelength measurements, information on the wavelength dependent backscattering of aerosol dust particles is required. The volume backscattering coefficient of aerosols in the IR is relatively small. Thus, only a few field measurements of backscattering, usually at only a few wavelengths, are reported in the literature. We present spectral field measurements of backscattering of kaolin dust in the 9-11 micron wavelength range. As the quantity of dust increases, multiple scattering contributes more to the measured backscattered signal. The measurements show the effect of the dust quantity of the spectral backscatter measurements. A simple analytical two stream radiative transfer model is applied to confirm the measurements and to give insight to the multiple scattering spectra of backscattering.

Immunoassays in Biotechnology

Science.gov (United States)

Immunoassays have broad applications for a wide variety of important biological compounds and environmental contaminants. Immunoassays can detect the presence of an antigen in the human body, a pollutant in the environment, or a critical antibody in a patient’s serum to develop a...

Retrieving Marine Inherent Optical Properties from Satellites Using Temperature and Salinity-dependent Backscattering by Seawater

Science.gov (United States)

Werdell, Paul J.; Franz, Bryan Alden; Lefler, Jason Travis; Robinson, Wayne D.; Boss, Emmanuel

2013-01-01

Time-series of marine inherent optical properties (IOPs) from ocean color satellite instruments provide valuable data records for studying long-term time changes in ocean ecosystems. Semi-analytical algorithms (SAAs) provide a common method for estimating IOPs from radiometric measurements of the marine light field. Most SAAs assign constant spectral values for seawater absorption and backscattering, assume spectral shape functions of the remaining constituent absorption and scattering components (e.g., phytoplankton, non-algal particles, and colored dissolved organic matter), and retrieve the magnitudes of each remaining constituent required to match the spectral distribution of measured radiances. Here, we explore the use of temperature- and salinity-dependent values for seawater backscattering in lieu of the constant spectrum currently employed by most SAAs. Our results suggest that use of temperature- and salinity-dependent seawater spectra elevate the SAA-derived particle backscattering, reduce the non-algal particles plus colored dissolved organic matter absorption, and leave the derived absorption by phytoplankton unchanged.

An analytical model for light backscattering by coccoliths and coccospheres of Emiliania huxleyi.

Science.gov (United States)

Fournier, Georges; Neukermans, Griet

2017-06-26

We present an analytical model for light backscattering by coccoliths and coccolithophores of the marine calcifying phytoplankter Emiliania huxleyi. The model is based on the separation of the effects of diffraction, refraction, and reflection on scattering, a valid assumption for particle sizes typical of coccoliths and coccolithophores. Our model results match closely with results from an exact scattering code that uses complex particle geometry and our model also mimics well abrupt transitions in scattering magnitude. Finally, we apply our model to predict changes in the spectral backscattering coefficient during an Emiliania huxleyi bloom with results that closely match in situ measurements. Because our model captures the key features that control the light backscattering process, it can be generalized to coccoliths and coccolithophores of different morphologies which can be obtained from size-calibrated electron microphotographs. Matlab codes of this model are provided as supplementary material.

Evaluation of a magnetic particles-based chemiluminescence enzyme immunoassay for Golgi protein 73 in human serum.

Science.gov (United States)

Liu, Xiangyi; Wan, Xiaohua; Lu, Sheng; Zhang, Lijun; Yu, Shaohua; Lu, Xinxin

2015-05-20

Golgi protein 73 (GP73) is regarded as a potential serum biomarker for early diagnosis of hepatocellular carcinoma (HCC). We developed a rapid magnetic particles-based chemiluminescence enzyme immunoassay (MPs-CLEIA) for the determination of serum GP73. Fluorescein isothiocyanate (FITC) and alkaline phosphatase (ALP) were used to label 2 different monoclonal antibodies to GP73. Serum GP73 was captured with labeled antibodies and formed a sandwiched immunoreaction. The magnetic particles (MPs) coated with anti-FITC antibody were used as a means of separation of the GP73 protein from other serum proteins. After adding the enzyme substrate solution, the relative light unit (RLU) was measured. A MPs-CLEIA for serum GP73 was established and evaluated. The RLU was directly proportional to the concentration of GP73. The method linearity was 5-600 μg/l. Limit of the blank was 2.19 μg/l. The intra- and inter-assay imprecision was 73-0.89), and the sensitivity and specificity, with cut-off value of 115.6 μg/l, were 75.4% and 92.1%, respectively. The proposed method demonstrates an acceptable performance for quantifying serum GP73. This assay could be appropriate for routine use in clinical laboratories. Copyright © 2015 Elsevier B.V. All rights reserved.

A digital instrument for nondestructive measurements of coating thicknesses by beta backscattering

Science.gov (United States)

Farcasiu, D. M.; Apostolescu, T.; Bozdog, H.; Badescu, E.; Bohm, V.; Stanescu, S. P.; Jianu, A.; Bordeanu, C.; Cracium, M. V.

1992-02-01

The elements of nondestructive gauging of coatings applied on various metal bases are presented. The intensity of the backscattered beta radiations is related to the thickness of the coating. With a fixed measuring geometry and radioactive sources (147Pm, 204Tl, 90Sr+90Y) the intensity of the backscattered beta particles is dependent on the following parameters: coating thickness, atomic number of the coating material and of the base, the beta particle energy and the surface finish. It can be used for the measurement of a wide range of coating thicknesses provided that the difference between the coating and the support atomic numbers is at least 20%. Fields of application include electronics, electrotechnique and so on.

A digital instrument for nondestructive measurements of coating thicknesses by beta backscattering

International Nuclear Information System (INIS)

Farcasiu, D.M.; Apostolescu, T.; Bozdog, H.; Badescu, E.; Bohm, V.; Stanescu, S.P.; Jianu, A.; Bordeanu, C.; Craciun, M.V.

1992-01-01

The elements of nondestructive gauging of coatings applied on various metal bases are presented. The intensity of the backscattered beta radiations is related to the thickness of the coating. With a fixed measuring geometry and radioactive sources ( 147 Pm, 204 Tl, 90 Sr+ 90 Y) the intensity of the backscattered beta particles is dependent on the following parameters: Coating thickness, atomic number of the coating material and of the base, the beta particle energy and the surface finish. It can be used for the measurement of a wide range of coating thicknesses provided that the difference between the coating and the support atomic numbers is at least 20%. Fields of application include electronics, electrotechnique and so on. (orig.)

Trace element determination in tomato puree using particle induced X-ray emission and Rutherford backscattering

International Nuclear Information System (INIS)

Romero-Davila, E.; Miranda, J.

2004-01-01

Particle induced X-ray emission (PIXE) and Rutherford backscattering spectrometry (RBS) were used to determine the concentrations of trace elements in samples of 12 tomato puree brands sold in the Mexican market. While RBS offered information about the main elements present in the matrix, PIXE gave results on trace elements. As a whole, data for 17 elements (C, N, O, Na, Mg, S, Cl, K, Ca, Ti, V, Cr, Mn, Fe, Ni, Cu, and Zn) were obtained. To evaluate the results, a comparison with brands from USA, Japan, Colombia, and Chile was carried out, using tomato purees produced following the domestic technology recipe. Additionally, the results were considered in the light of the Codex Alimentarius and the Mexican standard. It was found that all of the brands fall within the limits established by these standards, being of the same order of magnitude as the foreign brands. (author)

ILC beam energy measurement by means of laser Compton backscattering

Energy Technology Data Exchange (ETDEWEB)

Muchnoi, N. [Budker Inst. for Nuclear Physics, Novosibirsk (Russian Federation); Schreiber, H.J.; Viti, M. [Deutsches Elektronen-Synchrotron (DESY), Zeuthen (Germany)

2008-10-15

A novel, non-invasive method of measuring the beam energy at the International Linear Collider is proposed. Laser light collides head-on with beam particles and either the energy of the Compton scattered electrons near the kinematic end-point is measured or the positions of the Compton backscattered {gamma}-rays, the edge electrons and the unscattered beam particles are recorded. A compact layout for the Compton spectrometer is suggested. It consists of a bending magnet and position sensitive detectors operating in a large radiation environment. Several options for high spatial resolution detectors are discussed. Simulation studies support the use of an infrared or green laser and quartz fiber detectors to monitor the backscattered photons and edge electrons. Employing a cavity monitor, the beam particle position downstream of the magnet can be recorded with submicrometer precision. Such a scheme provides a feasible and promising method to access the incident beam energy with precisions of 10{sup -4} or better on a bunch-to-bunch basis while the electron and positron beams are in collision. (orig.)

ILC beam energy measurement by means of laser Compton backscattering

International Nuclear Information System (INIS)

Muchnoi, N.; Schreiber, H.J.; Viti, M.

2008-10-01

A novel, non-invasive method of measuring the beam energy at the International Linear Collider is proposed. Laser light collides head-on with beam particles and either the energy of the Compton scattered electrons near the kinematic end-point is measured or the positions of the Compton backscattered γ-rays, the edge electrons and the unscattered beam particles are recorded. A compact layout for the Compton spectrometer is suggested. It consists of a bending magnet and position sensitive detectors operating in a large radiation environment. Several options for high spatial resolution detectors are discussed. Simulation studies support the use of an infrared or green laser and quartz fiber detectors to monitor the backscattered photons and edge electrons. Employing a cavity monitor, the beam particle position downstream of the magnet can be recorded with submicrometer precision. Such a scheme provides a feasible and promising method to access the incident beam energy with precisions of 10 -4 or better on a bunch-to-bunch basis while the electron and positron beams are in collision. (orig.)

Specificity of immunoassays. Pt. 2

International Nuclear Information System (INIS)

Pratt, J.J.; Woldring, M.G.; Boonman, R.; Kittikool, J.

1979-01-01

Practical aspects of the measurement of the specificity of immunoassay are reviewed. Antibody heterogeneity in an antiserum makes a pragmatic rather than a theoretical approach necessary. A new method for the measurement of immunoassay specificity is described. This method is based on the errors caused by the cross-reacting antigens and is directly relevant to the validity of results obtained by immunoassay methods. The effect of selectively blocking the least specific antibodies in antisera raised against steroid haptens is tested. The practical consequences of these considerations are tested using steroid radioimmunoassay and enzyme-immunoassay. (orig.) [de

An Inexpensive, Fast and Sensitive Quantitative Lateral Flow Magneto-Immunoassay for Total Prostate Specific Antigen

Directory of Open Access Journals (Sweden)

Jacqueline M. Barnett

2014-07-01

Full Text Available We describe the detection characteristics of a device the Resonant Coil Magnetometer (RCM to quantify paramagnetic particles (PMPs in immunochromatographic (lateral flow assays. Lateral flow assays were developed using PMPs for the measurement of total prostate specific antigen (PSA in serum samples. A detection limit of 0.8 ng/mL was achieved for total PSA using the RCM and is at clinically significant concentrations. Comparison of data obtained in a pilot study from the analysis of serum samples with commercially available immunoassays shows good agreement. The development of a quantitative magneto-immunoassay in lateral flow format for total PSA suggests the potential of the RCM to operate with many immunoassay formats. The RCM has the potential to be modified to quantify multiple analytes in this format. This research shows promise for the development of an inexpensive device capable of quantifying multiple analytes at the point-of-care using a magneto-immunoassay in lateral flow format.

Suspended sediment assessment by combining sound attenuation and backscatter measurements - analytical method and experimental validation

Science.gov (United States)

Guerrero, Massimo; Di Federico, Vittorio

2018-03-01

The use of acoustic techniques has become common for estimating suspended sediment in water environments. An emitted beam propagates into water producing backscatter and attenuation, which depend on scattering particles concentration and size distribution. Unfortunately, the actual particles size distribution (PSD) may largely affect the accuracy of concentration quantification through the unknown coefficients of backscattering strength, ks2, and normalized attenuation, ζs. This issue was partially solved by applying the multi-frequency approach. Despite this possibility, a relevant scientific and practical question remains regarding the possibility of using acoustic methods to investigate poorly sorted sediment in the spectrum ranging from clay to fine sand. The aim of this study is to investigate the possibility of combining the measurement of sound attenuation and backscatter to determine ζs for the suspended particles and the corresponding concentration. The proposed method is moderately dependent from actual PSD, thus relaxing the need of frequent calibrations to account for changes in ks2 and ζs coefficients. Laboratory tests were conducted under controlled conditions to validate this measurement technique. With respect to existing approaches, the developed method more accurately estimates the concentration of suspended particles ranging from clay to fine sand and, at the same time, gives an indication on their actual PSD.

Single Particle Differentiation through 2D Optical Fiber Trapping and Back-Scattered Signal Statistical Analysis: An Exploratory Approach.

Science.gov (United States)

Paiva, Joana S; Ribeiro, Rita S R; Cunha, João P S; Rosa, Carla C; Jorge, Pedro A S

2018-02-27

Recent trends on microbiology point out the urge to develop optical micro-tools with multifunctionalities such as simultaneous manipulation and sensing. Considering that miniaturization has been recognized as one of the most important paradigms of emerging sensing biotechnologies, optical fiber tools, including Optical Fiber Tweezers (OFTs), are suitable candidates for developing multifunctional small sensors for Medicine and Biology. OFTs are flexible and versatile optotools based on fibers with one extremity patterned to form a micro-lens. These are able to focus laser beams and exert forces onto microparticles strong enough (piconewtons) to trap and manipulate them. In this paper, through an exploratory analysis of a 45 features set, including time and frequency-domain parameters of the back-scattered signal of particles trapped by a polymeric lens, we created a novel single feature able to differentiate synthetic particles (PMMA and Polystyrene) from living yeasts cells. This single statistical feature can be useful for the development of label-free hybrid optical fiber sensors with applications in infectious diseases detection or cells sorting. It can also contribute, by revealing the most significant information that can be extracted from the scattered signal, to the development of a simpler method for particles characterization (in terms of composition, heterogeneity degree) than existent technologies.

Specific absorption and backscatter coefficient signatures in southeastern Atlantic coastal waters

Science.gov (United States)

Bostater, Charles R., Jr.

1998-12-01

Measurements of natural water samples in the field and laboratory of hyperspectral signatures of total absorption and reflectance were obtained using long pathlength absorption systems (50 cm pathlength). Water was sampled in Indian River Lagoon, Banana River and Port Canaveral, Florida. Stations were also occupied in near coastal waters out to the edge of the Gulf Stream in the vicinity of Kennedy Space Center, Florida and estuarine waters along Port Royal Sound and along the Beaufort River tidal area in South Carolina. The measurements were utilized to calculate natural water specific absorption, total backscatter and specific backscatter optical signatures. The resulting optical cross section signatures suggest different models are needed for the different water types and that the common linear model may only appropriate for coastal and oceanic water types. Mean particle size estimates based on the optical cross section, suggest as expected, that particle size of oceanic particles are smaller than more turbid water types. The data discussed and presented are necessary for remote sensing applications of sensors as well as for development and inversion of remote sensing algorithms.

Coherent Backscattering in the Cross-Polarized Channel

Science.gov (United States)

Mischenko, Michael I.; Mackowski, Daniel W.

2011-01-01

We analyze the asymptotic behavior of the cross-polarized enhancement factor in the framework of the standard low-packing-density theory of coherent backscattering by discrete random media composed of spherically symmetric particles. It is shown that if the particles are strongly absorbing or if the smallest optical dimension of the particulate medium (i.e., the optical thickness of a plane-parallel slab or the optical diameter of a spherically symmetric volume) approaches zero, then the cross-polarized enhancement factor tends to its upper-limit value 2. This theoretical prediction is illustrated using direct computer solutions of the Maxwell equations for spherical volumes of discrete random medium.

Monoclonal antibody-based immunoassays.

Science.gov (United States)

Appleby, P; Reischl, U

1998-01-01

An immunoassay may be defined as an assay that employs an immunological reagent, usually an antibody, to confer specificity for the ligand being measured. As a corollary to this, the discovery, and subsequent development, of monoclonal antibodies (MAbs) has greatly expanded the application and use of immunoassays. Polyclonal reagents, with their associated problems of specificity and quality control, have now been largely replaced by readily available MAbs of potential immortality and well-defined specificity and affinity. This has resulted, in the last two decades, in a great expansion in the range of immunoassays available and also a significant improvement in their reproducibility and reliability.

Materials for Microfluidic Immunoassays: A Review.

Science.gov (United States)

Mou, Lei; Jiang, Xingyu

2017-08-01

Conventional immunoassays suffer from at least one of these following limitations: long processing time, high costs, poor user-friendliness, technical complexity, poor sensitivity and specificity. Microfluidics, a technology characterized by the engineered manipulation of fluids in channels with characteristic lengthscale of tens of micrometers, has shown considerable promise for improving immunoassays that could overcome these limitations in medical diagnostics and biology research. The combination of microfluidics and immunoassay can detect biomarkers with faster assay time, reduced volumes of reagents, lower power requirements, and higher levels of integration and automation compared to traditional approaches. This review focuses on the materials-related aspects of the recent advances in microfluidics-based immunoassays for point-of-care (POC) diagnostics of biomarkers. We compare the materials for microfluidic chips fabrication in five aspects: fabrication, integration, function, modification and cost, and describe their advantages and drawbacks. In addition, we review materials for modifying antibodies to improve the performance of the reaction of immunoassay. We also review the state of the art in microfluidic immunoassays POC platforms, from the laboratory to routine clinical practice, and also commercial products in the market. Finally, we discuss the current challenges and future developments in microfluidic immunoassays. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Advanced techniques in immunoassay

International Nuclear Information System (INIS)

Toth, G.

1982-01-01

A brief overview of the development history of radioimmunoassay and related techniques with their theory and practice are given. A comparison of radioimmunoassay (RIA), enzyme immunoassay (EIA), spin immunoassay (SIA), sequential saturation analysis (SSA) etc., based on their main parameters, and their fields of application and recent trends are presented. (Sz.J.)

Radiolabelling for immunoassay

International Nuclear Information System (INIS)

Chapman, R.S.

1998-01-01

Since the early 1960s labelled compounds employed in immunoassay techniques, both radioimmunoassay and immunoradiometric assay, have involved radioisotopes typically 3 H (tritium) and 125 Iodine. With the advent of increasingly stringent governmental regulations regarding usage and disposal of radioisotopes and the impetus of research towards improved immunoassay sensitivity following the discovery of monoclonal antibodies and their application to excess reagent immunometric assay methodology, radioisotopic labels are gradually being replaced by non-isotopic labels: enzyme, fluorescence and chemiluminescence

Backscattering Moessbauer spectroscopy of Martian dust

International Nuclear Information System (INIS)

Bertelsen, P.; Madsen, M. B.; Binau, C. S.; Goetz, W.; Gunnlaugsson, H. P.; Hviid, S. F.; Kinch, K. M.; Klingelhoefer, G.; Leer, K.; Madsen, D. E.; Merrison, J.; Olsen, M.; Squyres, S. W.

2005-01-01

We report on the determination of the mineralogy of the atmospherically suspended Martian dust particles using backscattering 57 Fe Moessbauer spectroscopy on dust accumulated onto the magnets onboard the Mars Exploration Rovers. The spectra can be interpreted in terms of minerals of igneous origin, and shows only limited, if any, amounts of secondary minerals that may have formed in the presence of liquid water. These findings suggest that the dust has formed in a dry environment over long time in the history of the planet.

Model for H- and D- production by hydrogen backscattering

International Nuclear Information System (INIS)

Hiskes, J.R.; Schneider, P.J.

1980-01-01

The Marlowe Monte-Carlo backscattering code has been used to calculate particle reflection coefficients and energy distributions for H, D incident upon Li, K, Ni, Cu, Mo, Ag, Cs, Hf, W, Pt, and U surfaces. The backscattered energy and angular distributions are combined with a model for formation and survival probabilities for H - , D - leaving the surface. A least-squares fit of experimental measurements of H - yields from the composite surface, Cs/Cu, has been used to obtain two semi-empirical constants, α, β which enter into the formation and survival probabilities. These probabilities are used to calculate the production probability which in turn provides an upper limit to the negative ion yield. The choice of electrode material is discussed as a function of atomic number

Immunoassay of paralytic shellfish toxins by moving magnetic particles in a stationary liquid-phase lab-on-a-chip.

Science.gov (United States)

Kim, Myoung-Ho; Choi, Suk-Jung

2015-04-15

In this study, we devised a stationary liquid-phase lab-on-a-chip (SLP LOC), which was operated by moving solid-phase magnetic particles in the stationary liquid phase. The SLP LOC consisted of a sample chamber to which a sample and reactants were added, a detection chamber containing enzyme substrate solution, and a narrow channel connecting the two chambers and filled with buffer. As a model system, competitive immunoassays of saxitoxin (STX), a paralytic shellfish toxin, were conducted in the SLP LOC using protein G-coupled magnetic particles (G-MPs) as the solid phase. Anti-STX antibodies, STX-horseradish peroxidase conjugate, G-MPs, and a STX sample were added to the sample chamber and reacted by shaking. While liquids were in the stationary state, G-MPs were transported from the sample chamber to the detection chamber by moving a magnet below the LOC. After incubation to allow the enzymatic reaction to occur, the absorbance of the detection chamber solution was found to be reciprocally related to the STX concentration of the sample. Thus, the SLP LOC may represent a novel, simple format for point-of-care testing applications of enzyme-linked immunosorbent assays by eliminating complicated liquid handling steps. Copyright © 2014 Elsevier B.V. All rights reserved.

Electromagnetic backscattering from one-dimensional drifting fractal sea surface II: Electromagnetic backscattering model

International Nuclear Information System (INIS)

Xie Tao; Zhao Shang-Zhuo; Fang He; Yu Wen-Jin; He Yi-Jun; Perrie, William

2016-01-01

Sea surface current has a significant influence on electromagnetic (EM) backscattering signals and may constitute a dominant synthetic aperture radar (SAR) imaging mechanism. An effective EM backscattering model for a one-dimensional drifting fractal sea surface is presented in this paper. This model is used to simulate EM backscattering signals from the drifting sea surface. Numerical results show that ocean currents have a significant influence on EM backscattering signals from the sea surface. The normalized radar cross section (NRCS) discrepancies between the model for a coupled wave-current fractal sea surface and the model for an uncoupled fractal sea surface increase with the increase of incidence angle, as well as with increasing ocean currents. Ocean currents that are parallel to the direction of the wave can weaken the EM backscattering signal intensity, while the EM backscattering signal is intensified by ocean currents propagating oppositely to the wave direction. The model presented in this paper can be used to study the SAR imaging mechanism for a drifting sea surface. (paper)

Backscattered Helium Spectroscopy in the Helium Ion Microscope: Principles, Resolution and Applications

NARCIS (Netherlands)

van Gastel, Raoul; Hlawacek, G.; Dutta, S.; Poelsema, Bene

2015-01-01

We demonstrate the possibilities and limitations for microstructure characterization using backscattered particles from a sharply focused helium ion beam. The interaction of helium ions with matter enables the imaging, spectroscopic characterization, as well as the nanometer scale modification of

The management of isolated positive syphilis enzyme immunoassay results in HIV-negative patients attending a sexual health clinic.

Science.gov (United States)

Thorley, Nicola; Adebayo, Michael; Smit, Erasmus; Radcliffe, Keith

2016-08-01

An unconfirmed positive treponemal enzyme immunoassay (enzyme immunoassay positive, Treponema pallidum particle agglutination negative and rapid plasma reagin negative) presents a clinical challenge to distinguish early syphilis infection from false-positive results. These cases are referred for syphilis line assay (INNO-LIA) and recalled for repeat syphilis serology. We performed a retrospective audit to establish the proportion of HIV-negative cases with unconfirmed positive enzyme immunoassay results, the proportion of these cases that received an INNO-LIA test and repeat syphilis serology testing and reviewed the clinical outcomes; 0.35% (80/22687) cases had an unconfirmed positive treponemal enzyme immunoassay result. Repeat syphilis serology was performed in 80% (64/80) cases, but no additional cases of syphilis were identified. Eighty-eight per cent (70/80) received an INNO-LIA test; 14% (5/37) unconfirmed enzyme immunoassay-positive cases with no prior history of syphilis were confirmed on INNO-LIA assay, supporting a diagnosis of latent syphilis. As a confirmatory treponemal test, the INNO-LIA assay may be more useful than repeat syphilis serological testing. © The Author(s) 2016.

Survey of immunoassay techniques for biological analysis

International Nuclear Information System (INIS)

Burtis, C.A.

1986-10-01

Immunoassay is a very specific, sensitive, and widely applicable analytical technique. Recent advances in genetic engineering have led to the development of monoclonal antibodies which further improves the specificity of immunoassays. Originally, radioisotopes were used to label the antigens and antibodies used in immunoassays. However, in the last decade, numerous types of immunoassays have been developed which utilize enzymes and fluorescent dyes as labels. Given the technical, safety, health, and disposal problems associated with using radioisotopes, immunoassays that utilize the enzyme and fluorescent labels are rapidly replacing those using radioisotope labels. These newer techniques are as sensitive, are easily automated, have stable reagents, and do not have a disposal problem. 6 refs., 1 fig., 2 tabs

Dynamic coherent backscattering mirror

Energy Technology Data Exchange (ETDEWEB)

Zeylikovich, I.; Xu, M., E-mail: mxu@fairfield.edu [Physics Department, Fairfield University, Fairfield, CT 06824 (United States)

2016-02-15

The phase of multiply scattered light has recently attracted considerable interest. Coherent backscattering is a striking phenomenon of multiple scattered light in which the coherence of light survives multiple scattering in a random medium and is observable in the direction space as an enhancement of the intensity of backscattered light within a cone around the retroreflection direction. Reciprocity also leads to enhancement of backscattering light in the spatial space. The random medium behaves as a reciprocity mirror which robustly converts a diverging incident beam into a converging backscattering one focusing at a conjugate spot in space. Here we first analyze theoretically this coherent backscattering mirror (CBM) phenomenon and then demonstrate the capability of CBM compensating and correcting both static and dynamic phase distortions occurring along the optical path. CBM may offer novel approaches for high speed dynamic phase corrections in optical systems and find applications in sensing and navigation.

Modeling radar backscattering from melting snowflakes using spheroids with nonuniform distribution of water

International Nuclear Information System (INIS)

Tyynelä, Jani; Leinonen, Jussi; Moisseev, Dmitri; Nousiainen, Timo; Lerber, Annakaisa von

2014-01-01

In a number of studies it is reported that at the early stages, melting of aggregate snowflakes is enhanced at lower parts. In this paper, the manifestation of the resulting nonuniform distribution of water is studied for radar backscattering cross sections at C, Ku, Ka and W bands. The melting particles are described as spheroids with a mixture of water and air at the bottom part of the particle and a mixture of ice and air at the upper part. The radar backscattering is modeled using the discrete-dipole approximation in a horizontally pointing geometry. The results are compared to the T-matrix method, Mie theory, and the Rayleigh approximation using the Maxwell Garnett mixing formula. We find that the differential reflectivity and the linear depolarization ratio show systematic differences between the discrete-dipole approximation and the T-matrix method, but that the differences are relatively small. The horizontal cross sections show only small differences between the methods with the aspect ratio and the presence of resonance peaks having a larger effect on it than the nonuniform distribution of water. Overall, the effect of anisotropic distribution of water, reported for early stages of melting, is not significant for radar observations at the studied frequencies. -- Highlights: • We model backscattering from spheroidal melting snowflakes at C, Ku, Ka, and W bands. • We study the effect of anisotropic distribution of meltwater in the snow particles. • We find systematic, but relatively small differences for the backscattering properties. • We find that the aspect ratio and resonance peaks have a bigger effect than anisotropic distribution of water. • Anisotropic distribution of water is not significant for radar observations at early stages of melting

Re-examining the effect of particle phase functions on the remote-sensing reflectance.

Science.gov (United States)

Xiong, Yuanheng; Zhang, Xiaodong; He, Shuangyan; Gray, Deric J

2017-08-20

Even though it is well known that both the magnitude and detailed angular shape of scattering (phase function, PF), particularly in the backward angles, affect the color of the ocean, the current remote-sensing reflectance (R rs ) models typically account for the effect of its magnitude only through the backscattering coefficient (b b ). Using 116 volume scattering function (VSF) measurements previously collected in three coastal waters around the U.S. and in the water of the North Atlantic Ocean, we re-examined the effect of particle PF on R rs in four scenarios. In each scenario, the magnitude of particle backscattering (i.e., b bp ) is known, but the knowledge on the angular shape of particle backscattering is assumed to increase from knowing nothing about the shape of particle PFs to partially knowing the particle backscattering ratio (B p ), the exact backscattering shape as defined by β˜ p (γ≥90°) (particle VSF normalized by the particle total scattering coefficient), and the exact backscattering shape as defined by the χ p factor (particle VSF normalized by the particle backscattering coefficient). At sun zenith angle=30°, the nadir-viewed R rs would vary up to 65%, 35%, 20%, and 10%, respectively, as the constraints on the shape of particle backscattering become increasingly stringent from scenarios 1 to 4. In all four scenarios, the R rs variations increase with both viewing and sun angles and are most prominent in the direction opposite the sun. Our results show a greater impact of the measured particle PFs on R rs than previously found, mainly because our VSF data show a much greater variability in B p , β˜ p (γ≥90°), and χ p than previously known. Among the uncertainties in R rs due to the particle PFs, about 97% can be explained by χ p , 90% by β˜ p (γ≥90°), and 27% by B p . The results indicate that the uncertainty in ocean color remote sensing can be significantly constrained by accounting for χ p of the VSFs.

Europium(III) chelate-dyed nanoparticles as donors in a homogeneous proximity-based immunoassay for estradiol

International Nuclear Information System (INIS)

Kokko, Leena; Sandberg, Kaisa; Loevgren, Timo; Soukka, Tero

2004-01-01

Nanoparticles containing thousands of fluorescent europium(III) chelates have a very high specific activity compared to traditional lanthanide chelate labels. It can be assumed that if these particles are used in a homogeneous assay as donors, multiple chelates can excite a single acceptor in turns and the energy transfer to the acceptor is increased. The principle was employed in an immunoassay using luminescent resonance energy transfer from a long lifetime europium(III) chelate-dyed nanoparticle to a short lifetime, near-infrared fluorescent molecule. Due to energy transfer fluorescence lifetime of the sensitised emission was prolonged and fluorescence could be measured using a time-resolved detection. A competitive homogeneous immunoassay for estradiol was created using 92 nm europium(III) chelate-dyed nanoparticle coated with 17β-estradiol specific recombinant antibody Fab fragments as a donor and estradiol conjugated with near-infrared dye AlexaFluor 680 as an acceptor. The density of Fab fragments on the surface of the particle influenced the sensitivity of the immunoassay. The optimal Fab density was reached when the entire surface of the particle participated in the energy transfer, but the areas where the energy was transferred to a single acceptor, did not overlap. We were able to detect estradiol concentrations down to 70 pmol l -1 (3xSD of a standard containing 0 nmol l -1 of E2) using a 96-well platform. In this study we demonstrated that nanoparticles containing lanthanide chelates could be used as efficient donors in homogeneous assays

MEASURING THE PARTICULATE BACKSCATTERING OF INLAND WATERS: A COMPARISON OF TECHNIQUES

Directory of Open Access Journals (Sweden)

G. Campbell

2012-07-01

Full Text Available The objective of this work was to examine whether the standard particulate backscattering IOP (Inherent Optical Properties measurement method could be simplified. IOP measurements are essential for parameterising several forms of algorithms used to estimate water quality parameters from airborne and satellite images. Field measurements of the backscattering IOPs are more difficult to make than absorption measurements as correction of the raw Hydroscat-6 backscattering sensor observations is required to allow for the systematic errors associated with the water and water quality parameter absorption. The standard approach involves making simultaneous measurement of the absorption and attenuation of the water with an absorption and attenuation meter (ac-9 or making assumptions about the particulate backscattering probability. Recently, a number of papers have been published that use an alternative method to retrieve the particulate backscattering spectrum by using laboratory measured absorption values and in situ spectroradiometric observations. The alternative method inverts a model of reflectance iteratively using non-linear least squares fitting to solve for the particulate backscattering at 532 nm (bbp0(532 and the particulate backscattering spectral slope (γ. In this paper, eleven observations made at Burdekin Falls Dam, Australia are used to compare the alternative reflectance method to the conventional corrected Hydroscat-6 observations. Assessment of the alternative reflectance method showed that the result of the inversions were highly dependent on the starting conditions. To overcome this limitation, Particle Swarm Optimisation, a stochastic search technique which includes a random element in the search approach, was used. It was found that when compared to the conventionally corrected Hydroscat-6 observations, the alternative reflectance method underestimated bbp0(532 by approximately 50% and overestimated γ by approximately 40

Light backscattering efficiency and related properties of some phytoplankters

Science.gov (United States)

Ahn, Yu-Hwan; Bricaud, Annick; Morel, André

1992-11-01

the interspecific variations in cellssize ( D) and intracellular pigment concentration ( Ci) (actually by the variations of the product DCi). Though b b∗ is involved in the modelling of the diffuse reflectance of waters, the impact of its actual variation is greatly limited because typical b b∗ values, even at their maximum (10 -3 m 2 mg -1), are very low. This result confirms that living algae have a negligible influence on the backscattering process by oceanic waters; other particles (bacteria, detritus, etc.) associated with algae are mainly responsible for this process.

A magnetic particle micro-trap for large trapping surfaces

KAUST Repository

Gooneratne, Chinthaka P.

2012-01-08

Manipulation of micron-size magnetic particles of the superparamagnetic type contributes significantly in many applications like controlling the antibody/antigen binding process in immunoassays. Specifically, more target biomolecules can be attached/tagged and analyzed since the three dimensional structure of the magnetic particles increases the surface to volume ratio. Additionally, such biomolecular-tagged magnetic particles can be easily manipulated by an external magnetic field due to their superparamagnetic behavior. Therefore, magnetic particle- based immunoassays are extensively applied in micro-flow cytometry. The design of a square-loop micro-trap as a magnetic particle manipulator as well as numerical and experimental analysis is presented. Experimental results showed that the micro-trap could successfully trap and concentrate magnetic particles from a large to a small area with a high spatial range.

A magnetic particle micro-trap for large trapping surfaces

KAUST Repository

Gooneratne, Chinthaka P.; Liang, Cai; Giouroudi, Ioanna; Kosel, Jü rgen

2012-01-01

Manipulation of micron-size magnetic particles of the superparamagnetic type contributes significantly in many applications like controlling the antibody/antigen binding process in immunoassays. Specifically, more target biomolecules can be attached/tagged and analyzed since the three dimensional structure of the magnetic particles increases the surface to volume ratio. Additionally, such biomolecular-tagged magnetic particles can be easily manipulated by an external magnetic field due to their superparamagnetic behavior. Therefore, magnetic particle- based immunoassays are extensively applied in micro-flow cytometry. The design of a square-loop micro-trap as a magnetic particle manipulator as well as numerical and experimental analysis is presented. Experimental results showed that the micro-trap could successfully trap and concentrate magnetic particles from a large to a small area with a high spatial range.

Corneal Backscatter Analysis by In Vivo Confocal Microscopy: Fellow Eye Comparison of Small Incision Lenticule Extraction and Femtosecond Laser-Assisted LASIK

Directory of Open Access Journals (Sweden)

Alper Agca

2014-01-01

Full Text Available Purpose. To evaluate and compare corneal backscatter from anterior stroma between small incision lenticule extraction (SMILE and femtosecond laser-assisted LASIK (femto-LASIK. Methods. A cohort of 60 eyes of 30 patients was randomized to receive SMILE in one eye and femto-LASIK in the fellow eye. In vivo confocal microscopy was performed at 1 week and 1, 3, and 6 months after surgery. The main outcome measurements were maximum backscattered intensity and the depth from which it was measured, the backscattered light intensity 30 μm below Bowman’s membrane at the flap interface and 150 μm below the superficial epithelium, and the number of refractive particles at the flap interface. Results. The mean backscattered light intensity (LI at all measured depths and the maximum backscattered LI were higher in the SMILE group than the femto-LASIK group at all postoperative visits. LI differences at 1 week and 1- and 3-month visits were statistically significant (P<0,05. LI differences at 6 months were not statistically significant. There was no difference in the number of refractive particles at the flap interface between the groups at any visit. Conclusions. SMILE results in increased backscattered LI in the anterior stroma when compared with femto-LASIK were evaluated.

Backscattering position detection for photonic force microscopy

International Nuclear Information System (INIS)

Volpe, Giovanni; Kozyreff, Gregory; Petrov, Dmitri

2007-01-01

An optically trapped particle is an extremely sensitive probe for the measurement of pico- and femto-Newton forces between the particle and its environment in microscopic systems (photonic force microscopy). A typical setup comprises an optical trap, which holds the probe, and a position sensing system, which uses the scattering of a beam illuminating the probe. Usually the position is accurately determined by measuring the deflection of the forward-scattered light transmitted through the probe. However, geometrical constraints may prevent access to this side of the trap, forcing one to make use of the backscattered light instead. A theory is presented together with numerical results that describes the use of the backscattered light for position detection. With a Mie-Debye approach, we compute the total (incident plus scattered) field and follow its evolution as it is collected by the condenser lenses and projected onto the position detectors and the responses of position sensitive detectors and quadrant photodetectors to the displacement of the probe in the optical trap, both in forward and backward configurations. We find out that in the case of backward detection, for both types of detectors the displacement sensitivity can change sign as a function of the probe size and is null for some critical sizes. In addition, we study the influence of the numerical aperture of the detection system, polarization, and the cross talk between position measurements in orthogonal directions. We finally discuss how these features should be taken into account in experimental designs

Particle optical backscattering along a chlorophyll gradient in the upper layer of the eastern South Pacific Ocean

Directory of Open Access Journals (Sweden)

Y. Huot

2008-04-01

Full Text Available The particulate scattering, b_p, and backscattering, b_bp, coefficients are determined by the concentration and physical properties of suspended particles in the ocean. They provide a simple description of the influence of these particles on the scattering of light within the water column. For the remote observation of ocean color, b_bp along with the total absorption coefficient govern the amount and spectral qualities of light leaving the sea surface. However, for the construction and validation of ocean color models measurements of b_bp are still lacking, especially at low chlorophyll a concentrations ([Chl]. Here, we examine the relationships between spectral b_bp and b_p vs. [Chl] along an 8000 km transect crossing the Case 1 waters of the eastern South Pacific Gyre. In these waters, over the entire range of [Chl] encountered (~0.02–2 mg m³, both b_bp and b_p can be related to [Chl] by power functions (i.e. b_p or b_bp=α[Chl]^β. Regression analyses are carried out to provide the parameters α and β for several wavelengths throughout the visible for both b_bp and b_p. When applied to the data, these functions retrieve the same fraction of variability in b_bp and b_p (coefficients of determination between 0.82 and 0.88. The b_bp coefficient fall within the bounds of previous measurements at intermediate and high [Chl] recently published. Its dependence on [Chl] below ~0.1 mg m⁻³ is described for the first time with in situ data. The backscattering ratio (i.e. b_bp/b_p with values near 0.01 for all stations appears to be spectrally neutral and not significantly dependent on [Chl]. These results should foster the

Light-scattering evolution from particles to regolith

International Nuclear Information System (INIS)

Videen, Gorden; Muinonen, Karri

2015-01-01

The radiative-transfer coherent-backscattering (RT–CB) model is unique among light-scattering methodologies as it can be used to calculate accurate light-scattering properties of sparsely populated particle volumes with sizes ranging from subwavelength to infinity. We use the RT–CB model to examine the evolution of light-scattering properties as a volume of particles increases from wavelength-sized to several hundreds of wavelengths. We examine the evolution of light-scattering intensity phase function and polarization, as well as linear and circular polarization ratios. We confirm the expected trends for backscattering features to shift to smaller phase angles as the volume increases. In addition, we also see the amplitude of these features increases to some maximum for volumes having size parameters kR∼100, before decaying to less than half this amplitude as their volumes approach infinity. - Highlights: • We use the RT–CB method to examine how gross light-scattering properties evolve as particle size increases. • The transition from a wavelength-sized particle to a large particle is not monotonic. • Backscattering properties associated with the CB mechanism appear to have a peak value before decaying asymptotically

The right environment for the immunoassay

International Nuclear Information System (INIS)

Emon, J.M. Van; Gerlach, C.L.

1995-01-01

For the US Environmental Protection Agency (EPA), the first in-house research effort began in 1987, when results of an early immunoassay field study verified the technology's potential for environmental applications. Looking at the fundamental features of immunochemical reactions from the clinical laboratories, analytical chemists realized the potential value of these methods for hazardous waste site characterization and pesticide monitoring. Immunoassays rely on the interaction between an antibody and a target analyte. For environmental purposes, enzyme immunoassays are generally used. After the target analyte binds to the antibody, an enzymatic reaction yields a colorimetric change. This change, read visually or by a spectrophotometer, indicates the concentration of the target analyte. Promising results with assays for compounds (such as paraquat and pentachlorophenol) and compound groups (such as total petroleum hydrocarbons and polychlorinated biphenyls) spurred interest among various entrepreneurs. The first target market for immunoassays was environmental engineers and field crews who needed quick answers on-site to determine the direction of further remediation efforts

Characterization of highly scattering media by measurement of diffusely backscattered polarized light

Science.gov (United States)

Hielscher, Andreas H.; Mourant, Judith R.; Bigio, Irving J.

2000-01-01

An apparatus and method for recording spatially dependent intensity patterns of polarized light that is diffusely backscattered from highly scattering media are described. These intensity patterns can be used to differentiate different turbid media, such as polystyrene-sphere and biological-cell suspensions. Polarized light from a He-Ne laser (.lambda.=543 nm) is focused onto the surface of the scattering medium, and a surface area of approximately 4.times.4 cm centered on the light input point is imaged through polarization analysis optics onto a CCD camera. A variety of intensity patterns may be observed by varying the polarization state of the incident laser light and changing the analyzer configuration to detect different polarization components of the backscattered light. Experimental results for polystyrene-sphere and Intralipid suspensions demonstrate that the radial and azimuthal variations of the observed pattern depend on the concentration, size, and anisotropy factor, g, of the particles constituting the scattering medium. Measurements performed on biological cell suspensions show that intensity patterns can be used to differentiate between suspensions of cancerous and non-cancerous cells. Introduction of the Mueller-matrix for diffusely backscattered light, permits the selection of a subset of measurements which comprehensively describes the optical properties of backscattering media.

The laser-backscattering equations and their application to the study of the atmospheric structure

CERN Document Server

Castrejon, R; Castrejon, J; Morales, A

2002-01-01

In this work a method for interpreting backscattering signals acquired by a lidar is described. The method is based on the elastic scattering of laser radiation due to gases and particles suspended in the atmosphere (bulk effects). We propose a space-time diagram which helps to evaluate the arguments of the equation that serves to calculate the lidar signal in terms of the backscattering coefficient. We describe how the system detects gradients on this coefficient, along the laser optical path. To illustrate the method, we present some typical lidar results obtained in the neighborhood of Mexico City. (Author)

Incoherent and coherent backscattering of light by a layer of densely packed random medium

Energy Technology Data Exchange (ETDEWEB)

Tishkovets, Victor P. [Institute of Radio Astronomy of NASU, 4 Chervonopraporna Street, Kharkiv 61002 (Ukraine)], E-mail: tishkovets@ira.kharkov.ua

2007-12-15

The problem of light scattering by a layer of densely packed discrete random medium is considered. The theory of light scattering by systems of nonspherical particles is applied to derive equations corresponding to incoherent (diffuse) and interference parts of radiation reflected from the medium. A solution of the system of linear equations describing light scattering by a system of particles is represented by iteration. It is shown that the symmetry properties of the T-matrices and of the translation coefficients for the vector Helmholtz harmonics lead to the reciprocity relation for an arbitrary iteration. This relation is applied to consider the backscattering enhancement phenomenon. Equations expressing the incoherent and interference parts of reflected light from statistically homogeneous and isotropic plane-parallel layer of medium are given. In the exact backscattering direction the relation between incoherent and interference parts is identical to that of sparse media.

Measurement of the thickness of thin films by backscattered protons

International Nuclear Information System (INIS)

Samaniego, L.E.Q.

1976-07-01

The method of backscattered protons has been used to measure the thickness of thin films. A monoenergetic beam of protons is directed on the film to be measured and the backscattered protons are detected with a particle detector. The film thickness is calculated from the energy spectrum of the protons. In the case of films consisting of several layers of elements with well separated atomic masses, it is possible to separate the spectra of protons scattered from the different elements, permitting a measurement of the thicknesses of the different layers. The method consists of calculating the energy loss of the protons throughout their trajectory, from the point of incidence on the film to the final detection. Thicknesses were measured for the following film combinations: gold on mylar, chromium on mylar, gold on chromium on mylar, and pure mylar. (Author) [pt

Composition of Renaissance paint layers: simultaneous particle induced X-ray emission and backscattering spectrometry.

Science.gov (United States)

de Viguerie, L; Beck, L; Salomon, J; Pichon, L; Walter, Ph

2009-10-01

Particle induced X-ray emission spectroscopy (PIXE) is now routinely used in the field of cultural heritage. Various setups have been developed to investigate the elemental composition of wood/canvas paintings or of cross-section samples. However, it is not possible to obtain information concerning the quantity of organic binder. Backscattering spectrometry (BS) can be a useful complementary method to overcome this limitation. In the case of paint layers, PIXE brings the elemental composition (major elements to traces) and the BS spectrum can give access to the proportion of pigment and binder. With the use of 3 MeV protons for PIXE and BS simultaneously, it was possible to perform quantitative analysis including C and O for which the non-Rutherford cross sections are intense. Furthermore, with the use of the same conditions for PIXE and BS, the experiment time and the potential damage by the ion beam were reduced. The results obtained with the external beam of the Accélérateur Grand Louvre pour l'Analyse Elementaire (AGLAE) facility on various test painting samples and on cross sections from Italian Renaissance masterpieces are shown. Simultaneous combination of PIXE and BS leads to a complete characterization of the paint layers: elemental composition and proportion of the organic binder have been determined and thus provide useful information about ancient oil painting recipes.

Highly sensitive immunoassay of protein molecules based on single nanoparticle fluorescence detection in a nanowell

Science.gov (United States)

Han, Jin-Hee; Kim, Hee-Joo; Lakshmana, Sudheendra; Gee, Shirley J.; Hammock, Bruce D.; Kennedy, Ian M.

2011-03-01

A nanoarray based-single molecule detection system was developed for detecting proteins with extremely high sensitivity. The nanoarray was able to effectively trap nanoparticles conjugated with biological sample into nanowells by integrating with an electrophoretic particle entrapment system (EPES). The nanoarray/EPES is superior to other biosensor using immunoassays in terms of saving the amounts of biological solution and enhancing kinetics of antibody binding due to reduced steric hindrance from the neighboring biological molecules. The nanoarray patterned onto a layer of PMMA and LOL on conductive and transparent indium tin oxide (ITO)-glass slide by using e-beam lithography. The suspension of 500 nm-fluorescent (green emission)-carboxylated polystyrene (PS) particles coated with protein-A followed by BDE 47 polyclonal antibody was added to the chip that was connected to the positive voltage. The droplet was covered by another ITO-coated-glass slide and connected to a ground terminal. After trapping the particles into the nanowells, the solution of different concentrations of anti-rabbit- IgG labeled with Alexa 532 was added for an immunoassay. A single molecule detection system could quantify the anti-rabbit IgG down to atto-mole level by counting photons emitted from the fluorescent dye bound to a single nanoparticle in a nanowell.

Chemiluminescence enzyme immunoassay based on magnetic nanoparticles for detection of hepatocellular carcinoma marker glypican-3

Directory of Open Access Journals (Sweden)

Qian-Yun Zhang

2011-08-01

Full Text Available Glypican-3 (GPC3 is reported as a great promising tumor marker for hepatocellular carcinoma (HCC diagnosis. Highly sensitive and accurate analysis of serum GPC3 (sGPC3, in combination with or instead of traditional HCC marker alpha-fetoprotein (AFP, is essential for early diagnosis of HCC. Biomaterial-functionalized magnetic particles have been utilized as solid supports with good biological compatibility for sensitive immunoassay. Here, the magnetic nanoparticles (MnPs and magnetic microparticles (MmPs with carboxyl groups were further modified with streptavidin, and applied for the development of chemiluminescence enzyme immunoassay (CLEIA. After comparing between MnPs- and MmPs-based CLEIA, MnPs-based CLEIA was proved to be a better method with less assay time, greater sensitivity, better linearity and longer chemiluminescence platform. MnPs-based CLEIA was applied for detection of sGPC3 in normal liver, hepatocirrhosis, secondary liver cancer and HCC serum samples. The results indicated that sGPC3 was effective in diagnosis of HCC with high performance. Keywords: Magnetic nanoparticle, Magnetic microparticle, Chemiluminescence enzyme immunoassay, Glypican-3, Hepatocellular carcinoma

Backscattering/transmission of 2 MeV He{sup ++} ions quantitative correlation study

Energy Technology Data Exchange (ETDEWEB)

Berec, V., E-mail: bervesn@gmail.com [Institute of Nuclear Sciences Vinca, University of Belgrade, P.O. Box 522, 11001 Belgrade (Serbia); Germogli, G.; Mazzolari, A.; Guidi, V. [INFN Sezione di Ferrara and Dipartimento di Fisica e Scienze della Terra, Via Saragat 1, 44100 Ferrara (Italy); De Salvador, D. [Dipartimento di Fisica, Università di Padova, Via Marzolo n.8, 35131 Padova (Italy); INFN Laboratori Nazionali di Legnaro, Viale Università 2, 35020 Legnaro, PD (Italy); Bacci, L. [INFN Laboratori Nazionali di Legnaro, Viale Università 2, 35020 Legnaro, PD (Italy)

2015-07-15

In this work we report on detailed findings of planar channeling oscillations of 2 MeV He{sup ++} particles in (1 1 0) silicon crystal. The exact correlation and coherence mechanism between confined particles oscillating trajectories are analyzed theoretically and experimentally in backscattering/transmission geometry. Regular patterns of channeled He{sup ++} ion planar oscillations are shown to be dominated by the crystal harmonic-oscillator potential and multiple scattering effect. For the first time it was shown that under the planar channeling conditions trajectories of positively charged particles exhibit observable correlation dynamics, including the interference effect. Quantitative estimation of channeling efficiency is performed using path integral method.

Rapid micromotor-based naked-eye immunoassay.

Science.gov (United States)

de Ávila, Berta Esteban-Fernández; Zhao, Mingjiao; Campuzano, Susana; Ricci, Francesco; Pingarrón, José M; Mascini, Marcello; Wang, Joseph

2017-05-15

A dynamic micromotor-based immunoassay, exemplified by cortisol detection, based on the use of tubular micromotors functionalized with a specific antibody is described. The use of antibody-functionalized micromotors offers huge acceleration of both direct and competitive cortisol immunoassays, along with greatly enhanced sensitivity of direct and competitive immunoassays. The dramatically improved speed and sensitivity reflect the greatly increased likelihood of antibody-cortisol contacts and fluid mixing associated with the dynamic movement of these microtube motors and corresponding bubble generation that lead to a highly efficient and rapid recognition process. Rapid naked-eye detection of cortisol in the sample is achieved in connection to use of horseradish peroxidase (HRP) tag and TMB/H 2 O 2 system. Key parameters of the competitive immunoassay (e.g., incubation time and reaction volume) were optimized. This fast visual micromotor-based sensing approach enables "on the move" specific detection of the target cortisol down to 0.1μgmL -1 in just 2min, using ultrasmall (50µL) sample volumes. Copyright © 2017 Elsevier B.V. All rights reserved.

Composite poly(methyl methacrylate-methacrylic acid-2-hydroxyethyl methacrylate) latex for immunoassay. The case of plasminogen.

Science.gov (United States)

Miksa, B; Wilczynska, M; Cierniewski, C; Basinska, T; Slomkowski, S

1995-01-01

Poly(methyl methacrylate-methacrylic acid-2-hydroxyethyl methacrylate) latex (ACRYLAT) was synthesized by radical precipitation polymerization. The mass median diameter (MMD) and the geometrical standard deviation (GSD) of the ACRYLAT particles were 138 nm and 1.2, respectively. The concentration of the titrable carboxylic groups in the surface layer of latex particles was equal to 8.41 x 10(-6) mol m-2. Latex was able to bind up to 2.82 x 10(-7) mol of 1-aminopyrene per 1 m2 of the surface of the latex particles due to the ionic interactions between carboxylate anions and ammonium cations of protonated 1-aminopyrene. ACRYLAT was able to immobilize covalently human serum albumin in amounts up to 0.23 mg m-2. Aggregation of ACRYLAT with immobilized HSA, induced with specific antibodies (anti-HSA), was investigated turbidimetrically. The results indicated that in the model turbidimetric immunoassay, ACRYLAT coated with HSA can be used for the detection of anti-HSA in the goat anti-HSA serum diluted from 50 to 7000-fold. Immobilization of rabbit antibodies to plasminogen (anti-Plg) to ACRYLAT via the epsilon-aminocaproic acid linkers provided particles which were used for the development of the turbidimetric immunoassay for plasminogen. In this assay plasminogen could be detected in concentration ranging from 0.75 to 75 micrograms ml-1 in the blood plasma.

Nanoparticle-based sandwich electrochemical immunoassay for carbohydrate antigen 125 with signal enhancement using enzyme-coated nanometer-sized enzyme-doped silica beads.

Science.gov (United States)

Tang, Dianping; Su, Biling; Tang, Juan; Ren, Jingjing; Chen, Guonan

2010-02-15

A novel nanoparticle-based electrochemical immunoassay of carbohydrate antigen 125 (CA125) as a model was designed to couple with a microfluidic strategy using anti-CA125-functionalized magnetic beads as immunosensing probes. To construct the immunoassay, thionine-horseradish peroxidase conjugation (TH-HRP) was initially doped into nanosilica particles using the reverse micelle method, and then HRP-labeled anti-CA125 antibodies (HRP-anti-CA125) were bound onto the surface of the synthesized nanoparticles, which were used as recognition elements. Different from conventional nanoparticle-based electrochemical immunoassays, the recognition elements of the immunoassay simultaneously contained electron mediator and enzyme labels and simplified the electrochemical measurement process. The sandwich-type immunoassay format was used for the online formation of the immunocomplex in an incubation cell and captured in the detection cell with an external magnet. The electrochemical signals derived from the carried HRP toward the reduction of H(2)O(2) using the doped thionine as electron mediator. Under optimal conditions, the electrochemical immunoassay exhibited a wide working range from 0.1 to 450 U/mL with a detection limit of 0.1 U/mL CA125. The precision, reproducibility, and stability of the immunoassay were acceptable. The assay was evaluated for clinical serum samples, receiving in excellent accordance with results obtained from the standard enzyme-linked immunosorbent assay (ELISA) method. Concluding, the nanoparticle-based assay format provides a promising approach in clinical application and thus represents a versatile detection method.

Backscatter measurements for NIF ignition targets (invited).

Science.gov (United States)

Moody, J D; Datte, P; Krauter, K; Bond, E; Michel, P A; Glenzer, S H; Divol, L; Niemann, C; Suter, L; Meezan, N; MacGowan, B J; Hibbard, R; London, R; Kilkenny, J; Wallace, R; Kline, J L; Knittel, K; Frieders, G; Golick, B; Ross, G; Widmann, K; Jackson, J; Vernon, S; Clancy, T

2010-10-01

Backscattered light via laser-plasma instabilities has been measured in early NIF hohlraum experiments on two beam quads using a suite of detectors. A full aperture backscatter system and near backscatter imager (NBI) instrument separately measure the stimulated Brillouin and stimulated Raman scattered light. Both instruments work in conjunction to determine the total backscattered power to an accuracy of ∼15%. In order to achieve the power accuracy we have added time-resolution to the NBI for the first time. This capability provides a temporally resolved spatial image of the backscatter which can be viewed as a movie.

Backscatter measurements for NIF ignition targets (invited)

Energy Technology Data Exchange (ETDEWEB)

Moody, J. D.; Datte, P.; Krauter, K.; Bond, E.; Michel, P. A.; Glenzer, S. H.; Divol, L.; Suter, L.; Meezan, N.; MacGowan, B. J.; Hibbard, R.; London, R.; Kilkenny, J.; Wallace, R.; Knittel, K.; Frieders, G.; Golick, B.; Ross, G.; Widmann, K.; Jackson, J. [Lawrence Livermore National Laboratory, P.O. Box 808, Livermore, California 94550 (United States); and others

2010-10-15

Backscattered light via laser-plasma instabilities has been measured in early NIF hohlraum experiments on two beam quads using a suite of detectors. A full aperture backscatter system and near backscatter imager (NBI) instrument separately measure the stimulated Brillouin and stimulated Raman scattered light. Both instruments work in conjunction to determine the total backscattered power to an accuracy of {approx}15%. In order to achieve the power accuracy we have added time-resolution to the NBI for the first time. This capability provides a temporally resolved spatial image of the backscatter which can be viewed as a movie.

Backscatter measurements for NIF ignition targets (invited)

International Nuclear Information System (INIS)

Moody, J. D.; Datte, P.; Krauter, K.; Bond, E.; Michel, P. A.; Glenzer, S. H.; Divol, L.; Suter, L.; Meezan, N.; MacGowan, B. J.; Hibbard, R.; London, R.; Kilkenny, J.; Wallace, R.; Knittel, K.; Frieders, G.; Golick, B.; Ross, G.; Widmann, K.; Jackson, J.

2010-01-01

Backscattered light via laser-plasma instabilities has been measured in early NIF hohlraum experiments on two beam quads using a suite of detectors. A full aperture backscatter system and near backscatter imager (NBI) instrument separately measure the stimulated Brillouin and stimulated Raman scattered light. Both instruments work in conjunction to determine the total backscattered power to an accuracy of ∼15%. In order to achieve the power accuracy we have added time-resolution to the NBI for the first time. This capability provides a temporally resolved spatial image of the backscatter which can be viewed as a movie.

Multibeam sonar backscatter data processing

Science.gov (United States)

Schimel, Alexandre C. G.; Beaudoin, Jonathan; Parnum, Iain M.; Le Bas, Tim; Schmidt, Val; Keith, Gordon; Ierodiaconou, Daniel

2018-06-01

Multibeam sonar systems now routinely record seafloor backscatter data, which are processed into backscatter mosaics and angular responses, both of which can assist in identifying seafloor types and morphology. Those data products are obtained from the multibeam sonar raw data files through a sequence of data processing stages that follows a basic plan, but the implementation of which varies greatly between sonar systems and software. In this article, we provide a comprehensive review of this backscatter data processing chain, with a focus on the variability in the possible implementation of each processing stage. Our objective for undertaking this task is twofold: (1) to provide an overview of backscatter data processing for the consideration of the general user and (2) to provide suggestions to multibeam sonar manufacturers, software providers and the operators of these systems and software for eventually reducing the lack of control, uncertainty and variability associated with current data processing implementations and the resulting backscatter data products. One such suggestion is the adoption of a nomenclature for increasingly refined levels of processing, akin to the nomenclature adopted for satellite remote-sensing data deliverables.

Retrieving simulated volcanic, desert dust and sea-salt particle properties from two/three-component particle mixtures using UV-VIS polarization lidar and T matrix

Directory of Open Access Journals (Sweden)

G. David

2013-07-01

Full Text Available During transport by advection, atmospheric nonspherical particles, such as volcanic ash, desert dust or sea-salt particles experience several chemical and physical processes, leading to a complex vertical atmospheric layering at remote sites where intrusion episodes occur. In this paper, a new methodology is proposed to analyse this complex vertical layering in the case of a two/three-component particle external mixtures. This methodology relies on an analysis of the spectral and polarization properties of the light backscattered by atmospheric particles. It is based on combining a sensitive and accurate UV-VIS polarization lidar experiment with T-matrix numerical simulations and air mass back trajectories. The Lyon UV-VIS polarization lidar is used to efficiently partition the particle mixture into its nonspherical components, while the T-matrix method is used for simulating the backscattering and depolarization properties of nonspherical volcanic ash, desert dust and sea-salt particles. It is shown that the particle mixtures' depolarization ratio δ p differs from the nonspherical particles' depolarization ratio δns due to the presence of spherical particles in the mixture. Hence, after identifying a tracer for nonspherical particles, particle backscattering coefficients specific to each nonspherical component can be retrieved in a two-component external mixture. For three-component mixtures, the spectral properties of light must in addition be exploited by using a dual-wavelength polarization lidar. Hence, for the first time, in a three-component external mixture, the nonsphericity of each particle is taken into account in a so-called 2β + 2δ formalism. Applications of this new methodology are then demonstrated in two case studies carried out in Lyon, France, related to the mixing of Eyjafjallajökull volcanic ash with sulfate particles (case of a two-component mixture and to the mixing of dust with sea-salt and water-soluble particles

Ion beam sputtering of Ti: Influence of process parameters on angular and energy distribution of sputtered and backscattered particles

Energy Technology Data Exchange (ETDEWEB)

Lautenschläger, T. [Leibniz-Institute of Surface Modification, 04318 Leipzig (Germany); Feder, R., E-mail: thomas.lautenschlaeger@iom-leipzig.de [Leibniz-Institute of Surface Modification, 04318 Leipzig (Germany); Neumann, H. [Leibniz-Institute of Surface Modification, 04318 Leipzig (Germany); Rice, C.; Schubert, M. [Department of Electrical and Computer Engineering and Center for Nanohybrid Functional Materials, University of Nebraska-Lincoln, Lincoln, Nebraska 68588-0511 (United States); Bundesmann, C. [Leibniz-Institute of Surface Modification, 04318 Leipzig (Germany)

2016-10-15

Highlights: • Ion beam sputter deposition under systematic variation of process parameters. • Angular and energy distribution of secondary particles. • Interaction between incorporated and impinging process gas. • Measured data compared with simulations. - Abstract: In the present study, the influence of ion energy and geometrical parameters onto the angular and energy distribution of secondary particles for sputtering a Ti target with Ar ions is investigated. The angular distribution of the particle flux of the sputtered Ti atoms was determined by the collection method, i.e. by growing Ti films and measuring their thickness. The formal description of the particle flux can be realized by dividing it into an isotropic and an anisotropic part. The experimental data show that increasing the ion energy or decreasing the ion incidence angle lead to an increase of the isotropic part, which is in good agreement with basic sputtering theory. The energy distribution of the secondary ions was measured using an energy-selective mass spectrometer. The energy distribution of the sputtered target ions shows a maximum at an energy between 10 eV and 20 eV followed by a decay proportional to E{sup −n}, which is in principle in accordance with Thompson’s theory, followed by a high energetic tail. When the sum of incidence angle and emission angle is increased, the high-energetic tail expands to higher energies and an additional peak due to direct sputtering events may occur. In the case of backscattered primary Ar ions, a maximum at an energy between 5 eV and 10 eV appears and, depending on the scattering geometry, an additional broad peak at a higher energy due to direct scattering events is observed. The center energy of the additional structure shifts systematically to higher energies with decreasing scattering angle or increasing ion energy. The experimental results are compared to calculations based on simple elastic two-particle-interaction theory and to

Can LC and LC-MS ever replace immunoassays?

Directory of Open Access Journals (Sweden)

Timothy G. Cross

2016-10-01

Full Text Available Immunoassays have been the technology of choice for the analysis of biomolecules for many decades across a wide range of applications in research, diagnostics and infectious disease monitoring. There are good reasons for the wide adoption of immunoassays but even such a well established and characterised technique has limitations and as such investigators are looking at alternative technologies. One such alternative is liquid chromatography (LC and, more specifically, liquid chromatography coupled with mass spectrometry (LC-MS. This article will review both immunoassay and LC and LC-MS technologies and methodologies and discuss the advantages and limitations of both approaches. In addition, the next developments that will need to occur before there is widespread adoption of LC and LC-MS technology preferentially over immunoassays will be examined.

Status of immunoassay as an analytical tool in environmental investigations

International Nuclear Information System (INIS)

Van Emon, J.M.

2000-01-01

Immunoassay methods were initially applied in clinical situations where their sensitivity and selectivity were utilized for diagnostic purposes. In the 1970s, pesticide chemists realized the potential benefits of immunoassay methods for compounds difficult to analyze by gas chromatography. This transition of the technology has extended to the analysis of soil, water, food and other matrices of environmental and human exposure significance particularly for compounds difficult to analyze by chromatographic methods. The utility of radioimmunoassays and enzyme immunoassays for environmental investigations was recognized in the 1980s by the U.S. Environmental Protection Agency (U.S. EPA) with the initiation of an immunoassay development programme. The U.S. Department of Agriculture (USDA) and the U.S. Food and Drug Administration (PDA) have investigated immunoassays for the detection of residues in food both from an inspection and a contamination prevention perspective. Environmental immunoassays are providing rapid screening information as well as quantitative information to fulfill rigorous data quality objectives for monitoring programmes

Catch and measure-mass spectrometry-based immunoassays in biomarker research.

Science.gov (United States)

Weiß, Frederik; van den Berg, Bart H J; Planatscher, Hannes; Pynn, Christopher J; Joos, Thomas O; Poetz, Oliver

2014-05-01

Mass spectrometry-based (MS) methods are effective tools for discovering protein biomarker candidates that can differentiate between physiological and pathophysiological states. Promising candidates are validated in studies comprising large patient cohorts. Here, targeted protein analytics are used to increase sample throughput. Methods involving antibodies, such as sandwich immunoassays or Western blots, are commonly applied at this stage. Highly-specific and sensitive mass spectrometry-based immunoassays that have been established in recent years offer a suitable alternative to sandwich immunoassays for quantifying proteins. Mass Spectrometric ImmunoAssays (MSIA) and Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA/iMALDI) are two prominent types of MS-based immunoassays in which the capture is done either at the protein or the peptide level. We present an overview of these emerging types of immunoassays and discuss their suitability for the discovery and validation of protein biomarkers. This article is part of a Special Issue entitled: Biomarkers: A Proteomic Challenge. © 2013.

Absorption and backscatter of internal conversion electrons in the measurements of surface contamination of 137Cs

International Nuclear Information System (INIS)

Yunoki, A.; Kawada, Y.; Yamada, T.; Unno, Y.; Sato, Y.; Hino, Y.

2013-01-01

We measured 4π and 2π counting efficiencies for internal conversion electrons (ICEs), gross β-particles and also β-rays alone with various source conditions regarding absorber and backing foil thickness using e-X coincidence technique. Dominant differences regarding the penetration, attenuation and backscattering properties among ICEs and β-rays were revealed. Although the abundance of internal conversion electrons of 137 Cs- 137 Ba is only 9.35%, 60% of gross counts may be attributed to ICEs in worse source conditions. This information will be useful for radionuclide metrology and for surface contamination monitoring. - Highlights: • Counting efficiencies for internal conversion electrons from 137 Cs were measured, and compared with those for β-rays. • Electron-X coincidence technique was employed. • A thin NaI(Tl) scintillation detector was used for X-ray detection. • Backscattering fractions of electrons and beta particles were studied by similar experiments

Spatially coded backscatter radiography

International Nuclear Information System (INIS)

Thangavelu, S.; Hussein, E.M.A.

2007-01-01

Conventional radiography requires access to two opposite sides of an object, which makes it unsuitable for the inspection of extended and/or thick structures (airframes, bridges, floors etc.). Backscatter imaging can overcome this problem, but the indications obtained are difficult to interpret. This paper applies the coded aperture technique to gamma-ray backscatter-radiography in order to enhance the detectability of flaws. This spatial coding method involves the positioning of a mask with closed and open holes to selectively permit or block the passage of radiation. The obtained coded-aperture indications are then mathematically decoded to detect the presence of anomalies. Indications obtained from Monte Carlo calculations were utilized in this work to simulate radiation scattering measurements. These simulated measurements were used to investigate the applicability of this technique to the detection of flaws by backscatter radiography

Laboratory evaluation of the Sequoia Scientific LISST-ABS acoustic backscatter sediment sensor

Science.gov (United States)

Snazelle, Teri T.

2017-12-18

Sequoia Scientific’s LISST-ABS is an acoustic backscatter sensor designed to measure suspended-sediment concentration at a point source. Three LISST-ABS were evaluated at the U.S. Geological Survey (USGS) Hydrologic Instrumentation Facility (HIF). Serial numbers 6010, 6039, and 6058 were assessed for accuracy in solutions with varying particle-size distributions and for the effect of temperature on sensor accuracy. Certified sediment samples composed of different ranges of particle size were purchased from Powder Technology Inc. These sediment samples were 30–80-micron (µm) Arizona Test Dust; less than 22-µm ISO 12103-1, A1 Ultrafine Test Dust; and 149-µm MIL-STD 810E Silica Dust. The sensor was able to accurately measure suspended-sediment concentration when calibrated with sediment of the same particle-size distribution as the measured. Overall testing demonstrated that sensors calibrated with finer sized sediments overdetect sediment concentrations with coarser sized sediments, and sensors calibrated with coarser sized sediments do not detect increases in sediment concentrations from small and fine sediments. These test results are not unexpected for an acoustic-backscatter device and stress the need for using accurate site-specific particle-size distributions during sensor calibration. When calibrated for ultrafine dust with a less than 22-µm particle size (silt) and with the Arizona Test Dust with a 30–80-µm range, the data from sensor 6039 were biased high when fractions of the coarser (149-µm) Silica Dust were added. Data from sensor 6058 showed similar results with an elevated response to coarser material when calibrated with a finer particle-size distribution and a lack of detection when subjected to finer particle-size sediment. Sensor 6010 was also tested for the effect of dissimilar particle size during the calibration and showed little effect. Subsequent testing revealed problems with this sensor, including an inadequate temperature

Rapid determination of recent cocaine use with magnetic particles-based enzyme immunoassays in serum, saliva, and urine fluids.

Science.gov (United States)

Vidal, Juan C; Bertolín, Juan R; Bonel, Laura; Asturias, Laura; Arcos-Martínez, M Julia; Castillo, Juan R

2016-06-05

Cocaine is one of the most worldwide used illicit drugs. We report a magnetic particles-based enzyme-linked immunoassay (mpEIA) method for the rapid and sensitive determination of cocaine (COC) in saliva, urine and serum samples. Under optimized conditions, the limits of detections were 0.09ngmL(-1) (urine), 0.15ngmL(-1) (saliva), and 0.06ngmL(-1) COC (human serum). Sensitivities were in the range EC50=0.6-2.5ngmL(-1) COC. The cross-reactivity with the principal metabolite benzoylecgonine (BZE) was only 1.6%. Recovering percentages of doped samples (0, 10, 50, and 100ngmL(-1) of COC) ranged from about 86-111%. Some advantages of the developed mpEIA over conventional ELISA kits are faster incubations, improved reproducibility, and consumption of lower amounts of antibody and enzyme conjugates due to the use of magnetic beads. The reported method was validated following the guidelines on bioanalytical methods of the European Medicines Agency (2011). Unmetabolized COC detection has a great interest in pharmacological, pharmacokinetics, and toxicokinetics studies, and can be used to detect a very recent COC use (1-6h). Copyright © 2016 Elsevier B.V. All rights reserved.

Magnetic particles in medical research - a review

International Nuclear Information System (INIS)

Sajid, K.M.

2001-01-01

Magnetic (or magnetizable) particles have assumed increasing importance in medical and biological research since 1966 when the effect of a magnetic field on the movement of suspended particles was initially studied. In fields like haematology, cell biology, microbiology, biochemistry and immunoassays, they currently provide the basis for separation techniques, which previously relied on gravitational forces. The body cells (e.g., blood cells) can be made magnetic by incubating them in a medium containing several Fe/sub 3/O/sub 4/ particles, which are adsorbed to the membrane surfaces. Some bacteria (also called magnetostatic bacteria) respond to externally applied magnetic lines of force due to their intracellular magnetic particles. These properties are useful in the isolation of these cells/bacteria. In biochemistry magnetic particles are used to immobilize enzymes without any loss of enzyme activity. The immobilized enzymes can facilitate the separation of end products without extensive instrumentation. In immunoassays the antibodies are covalently linked to polymer coated iron oxide particles. An electromagnet is used to sediment these particles after reaction. This excludes the use of centrifuge to separate antigen-antibody complexes. In pharmacy and pharmacology the magnetic particles are important in drug transport. In techniques like ferrography, nuclear magnetic resonance imaging (NMRI), spectroscopic studies and magnetic resonance imaging (MRI) the magnetic particles serve as contrast agents and give clinically important spatial resolution. Magnetic particles also find extensive applications in cancer therapy, genetic engineering, pneumology, nuclear medicine, radiology and many other fields. This article reviews these applications. (author)

Bead-based immunoassays

NARCIS (Netherlands)

Wal, van der F.J.; Bergervoet, J.H.W.; Achterberg, R.P.; Haasnoot, W.

2014-01-01

Since the first immunoassay with (radioactive) labeled antibodies in the middle of the 20th century [1], many different formats on various platforms have been developed, using antibodies for capture and/or detection. If antibodies are used to capture compounds, a support, such as the wall of a

Streptavidin-functionalized capillary immune microreactor for highly efficient chemiluminescent immunoassay

Energy Technology Data Exchange (ETDEWEB)

Yang Zhanjun [State Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093 (China); College of Chemistry and Engineering, Yangzhou University, 88 South University Avenue, Yangzhou 225002 (China); Zong Chen [State Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093 (China); Ju Huangxian, E-mail: hxju@nju.edu.cn [State Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093 (China); Yan Feng, E-mail: yanfeng2007@sohu.com [Jiangsu Institute of Cancer Prevention and Cure, Nanjing 210009 (China)

2011-11-07

Highlights: {yields} A novel capillary immune microreactor was proposed for highly efficient flow-through chemiluminescent immunoassay. {yields} The microreactor was prepared by functionalizing capillary inner wall with streptavidin for capture of biotinylated antibody. {yields} The proposed immunoassay method showed wide dynamic range, good reproducibility, stability and practicality. {yields} The microreactor was low-cost and disposable, and possessed several advantages over the conventional immunoreactors. - Abstract: A streptavidin functionalized capillary immune microreactor was designed for highly efficient flow-through chemiluminescent (CL) immunoassay. The functionalized capillary could be used as both a support for highly efficient immobilization of antibody and a flow cell for flow-through immunoassay. The functionalized inner wall and the capture process were characterized using scanning electron microscopy. Compared to conventional packed tube or thin-layer cell immunoreactor, the proposed microreactor showed remarkable properties such as lower cost, simpler fabrication, better practicality and wider dynamic range for fast CL immunoassay with good reproducibility and stability. Using {alpha}-fetoprotein as model analyte, the highly efficient CL flow-through immunoassay system showed a linear range of 3 orders of magnitude from 0.5 to 200 ng mL{sup -1} and a low detection limit of 0.1 ng mL{sup -1}. The capillary immune microreactor could make up the shortcoming of conventional CL immunoreactors and provided a promising alternative for highly efficient flow-injection immunoassay.

Linear Depolarization of Lidar Returns by Aged Smoke Particles

Science.gov (United States)

Mishchenko, Michael I.; Dlugach, Janna M.; Liu, Li

2016-01-01

We use the numerically exact (superposition) T-matrix method to analyze recent measurements of the backscattering linear depolarization ratio (LDR) for a plume of aged smoke at lidar wavelengths ranging from 355 to 1064 nm. We show that the unique spectral dependence of the measured LDRs can be modeled, but only by assuming expressly nonspherical morphologies of smoke particles containing substantial amounts of nonabsorbing (or weakly absorbing) refractory materials such as sulfates. Our results demonstrate that spectral backscattering LDR measurements can be indicative of the presence of morphologically complex smoke particles, but additional (e.g., passive polarimetric or bistatic lidar) measurements may be required for a definitive characterization of the particle morphology and composition.

Comparison of capillary electrophoresis-based immunoassay with fluorescence polarization immunoassay for the immunodetermination of methamphetamine using various methamphetamine antibodies.

Science.gov (United States)

Choi, J; Kim, C; Choi, M J

1998-11-01

An accurate and simple immunoassay using capillary electrophoresis (CE) with laser-induced fluorescence (LIF) was performed for the detection of methamphetamine (MA) in urine. The CE-LIF was conducted with an untreated fused-silica column using antiserum and a tracer of fluorescein isothiocyanate (FITC)-labeled MA. This CE-LIF system was compared with fluorescence polarization immunoassay (FPIA) in a TDx analyzer in the photo-check mode using the same FITC-labeled tracer and the same antiserum. Various antibodies, not only those prepared by our own immunogens but also those from commercial sources, were screened and characterized in both assay systems with regard to sensitivity, precision, and cross-reactivity. Both systems satisfied analytical precision and gave similar cross-reactivity patterns. However, the CE-LIF-based immunoassay was approximately one order superior to FPIA in sensitivity, requiring less volume of sample, antiserum, and tracer for the assay. Considering that the FPIA system is well known to be a useful tool for screening antibodies and detecting drugs, the CE-LIF-based immunoassay system, which is seemingly more advantageous than the FPIA system, appears to have great power for the characterization of antibodies and for the detection of MA in urine.

Calibrating a ground-based backscatter lidar for continuous measurements of PM2.5

Science.gov (United States)

Pesch, Markus; Oderbolz, Daniel

2007-10-01

One of the main issues of atmospheric research and air quality control is the reduction of harmful particulate matter (PM) in the atmosphere. Small particles can enter the human airways and cause serious health problems such as COPD (Chronic Obstructive Pulmonary Disease), asthma or even lung cancer. Recently, interest has shifted from PM10 to finer fractions of particulate matter, e.g. PM2.5, because the health impact of finer particles is considered to be more severe. Up to now measurements of particulate matter were carried out mainly at ground level. However important atmospheric processes, i.e. particle formation, transport and vertical mixing processes, take place predominantly at higher altitudes in the planetary boundary layer. Lidar in principle provides the ability to observe these processes where they occur. The new method outlined in this paper demonstrates the use of a small sized and quite inexpensive lidar in stand-alone operation to investigate transport processes of particulate matter, and PM2.5 in particular. Continuous measurements of PM2.5 as a reference are gained with a conventional in-situ monitor, installed on a tower at an altitude of 325 m in the North of Berlin (Frohnauer Turm). These PM2.5 measurements will be compared with backscatter Lidar data (1064 nm) taken from approx. 60 m over ground up to an altitude of 15 km with a spatial resolution of 15 m. The vertical backscatter profiles at 325 m will be correlated to the concentrations obtained by the PM2,5 monitor on the tower. Both measurements have a time resolution of 180 s to observe also processes that take place at short time scales. The objective is to gain correlation functions for estimating PM2.5 concentrations from backscatter Lidar data. Such a calibrated Lidar system is a valuable instrument for environmental agencies and atmospheric research groups to observe and investigate causes of high level PM concentrations. First results show a reasonably good linear correlation

The use of ion beam analysis in the synthesis of materials. A review of the determination of light elements by high-energy resonant backscattering

International Nuclear Information System (INIS)

Nastasi, M.

1999-01-01

For the ion synthesis of materials plasma inversion ion processing (PIIP) technology, has been applied in the framework of materials science research. The characteristics of this technique are discussed. PIIP has been complemented and compared with alpha-particle Rutherford Backscattering Spectrometry (RBS) High-energy Backscattering Spectrometry (HEBS) is the third material testing method for comparison. Examples for each technology are presented and discussed. (R.P.)

Main types of optical beams giving predominant contributions to the light backscatter for the irregular hexagonal columns

Science.gov (United States)

Shishko, Victor A.; Konoshonkin, Alexander V.; Kustova, Natalia V.; Borovoi, Anatoli G.

2017-11-01

This work presents the estimation of contribution of the main types of optical beams to the light backscatter for randomly oriented hexagonal ice column, the right dihedral angle of which was distorted within the range of 0° (regular particle) to 10°. Calculations were obtained within the physical optics approximation. The wavelength was 532 nm and the refractive index was 1.3116. The results showed that the total contribution of the main types of optical beams to the total backscattering cross section reach the value of 85% at small distortion angle of the hexagonal column and at substantial distortion angle the total contribution of the main types of optical beams decrease up to 55% of the total backscattering cross section. The obtained conclusions can significantly reduce the calculation time in the case when there is no need for high accuracy of the calculation.

Recent advancements in the immunoassay domain

International Nuclear Information System (INIS)

Pradelles, Ph.

1997-01-01

The two types of immunoassay techniques, the competition analysis and the immuno-metric analysis (sandwich type), are described; the tracers used with theses methods have high specific radioactivity levels in order to be traced at extremely low content. Non radioactive tracers have been also developed, such as enzymatic, fluorescent, luminescent tracers, which are simpler and may be used at home. The Cea has recently developed some innovative immunoassay formats, such as acetylcholinesterase as a new enzymatic tracer, and immuno-metric dosage for very small molecules such as haptenes

Relating P-band AIRSAR backscatter to forest stand parameters

Science.gov (United States)

Wang, Yong; Melack, John M.; Davis, Frank W.; Kasischke, Eric S.; Christensen, Norman L., Jr.

1993-01-01

As part of research on forest ecosystems, the Jet Propulsion Laboratory (JPL) and collaborating research teams have conducted multi-season airborne synthetic aperture radar (AIRSAR) experiments in three forest ecosystems including temperate pine forest (Duke, Forest, North Carolina), boreal forest (Bonanza Creek Experimental Forest, Alaska), and northern mixed hardwood-conifer forest (Michigan Biological Station, Michigan). The major research goals were to improve understanding of the relationships between radar backscatter and phenological variables (e.g. stand density, tree size, etc.), to improve radar backscatter models of tree canopy properties, and to develop a radar-based scheme for monitoring forest phenological changes. In September 1989, AIRSAR backscatter data were acquired over the Duke Forest. As the aboveground biomass of the loblolly pine forest stands at Duke Forest increased, the SAR backscatter at C-, L-, and P-bands increased and saturated at different biomass levels for the C-band, L-band, and P-band data. We only use the P-band backscatter data and ground measurements here to study the relationships between the backscatter and stand density, the backscatter and mean trunk dbh (diameter at breast height) of trees in the stands, and the backscatter and stand basal area.

Review of the biochemical basis of enzyme immunoassays

International Nuclear Information System (INIS)

Klingler, W.

1982-01-01

The ever increasing number of radioimmunological determination poses problems allied with the handling of radioactive substances. In recent years various non-radioactive methods have been developed, among which the enzyme immunoassay is already in routine use. Homogeneous and heterogeneous enzyme immunoassays are described. Criteria for enzymes, substrates and enzyme-substrate reactions are listed. (orig.) [de

Backscattering Properties of Nonspherical Ice Particles Calculated by Geometrical-Optics-Integral-Equation Method

Directory of Open Access Journals (Sweden)

Masuda Kazuhiko

2016-01-01

Full Text Available Backscattering properties of ice crystal models (Voronoi aggregates (VA, hexagonal columns (COL, and six-branched bullet rosettes (BR6 are calculated by using geometrical-opticsintegral-equation (GOIE method. Characteristics of depolarization ratio (δ and lidar ratio (L of the crystal models are examined. δ (L values are 0.2~0.3 (4~50, 0.3~0.4 (10~25, and 0.5~0.6 (50~100 for COL, BR6, and VA, respectively, at wavelength λ=0.532 μm. It is found that small deformation of COL model could produce significant changes in δ and L.

Understanding the radar backscattering from flooded and nonflooded Amazonian forests: results from canopy backscatter modeling

International Nuclear Information System (INIS)

Wang, Y.; Hess, L.L.; Filoso, S.; Melack, J.M.

1995-01-01

To understand the potential of using multiwavelength imaging radars to detect flooding in Amazonian floodplain forests, we simulated the radar backscatter from a floodplain forest with a flooded or nonflooded ground condition at C-, L-, and P-bands. Field measurements of forest structure in the Anavilhanas archipelago of the Negro River, Brazil, were used as inputs to the model. Given the same wavelength or incidence angle, the ratio of backscatter from the flooded forest to that from the nonflooded forest was higher at HH polarization than at VV polarization. Given the same wavelength or polarization, the ratio was larger at small incidence angles than at large incidence angles. Given the same polarization or incidence angle, the ratio was larger at a long wavelength than at a short wavelength. As the surface soil moisture underneath the nonflooded forest increased from 10% to 50% of volumetric moisture, the flooded/nonflooded backscatter ratio decreased; the decreases were small at C- and L-band but large at P-band. When the leaf size was comparable to or larger than the wavelength of C-band, the leaf area index (LAI) had a large effect on the simulated C-band (not L-band or P-band) backscatter from the flooded and nonflooded forests. (author)

Chemiluminescence immunoassay for chloramphenicol

International Nuclear Information System (INIS)

Lin Si; Xu Wenge; Liu Yibing

2007-06-01

A simple, solid-phase chemiluminescence immunoassay (CLIA) for the measurement of Chloramphenicol(CAP) in foodstuffs is described. A rabbit anti-CAP IgG is passively adsorbed onto the walls of polypropylene plates. The labeled conjugant is horseradish peroxidase(HRP) conjugate of CAP. Luminol solution is used as the substrate of HRP. The light yield is inversely proportional to the concentration of CAP. The method has a similar sensitivity (0.05 ng/mL), specificity, precision, and accuracy to a conventional enzyme immunoassay (EIA). The intra-assay and inter-assay CVs of ten samples were <8 and <20%, respectively, and the analytical recovery of the method was 87% 100%. The experimental correlation coefficient of dilution was found to be 0.999 using milk supernatant as buffer. The assay range for the method was 0.1-10 ng/mL, and it displayed good linearity. (authors)

Nodule bottom backscattering study using multibeam echosounder

Digital Repository Service at National Institute of Oceanography (India)

Chakraborty, B.; Raju, Y.S.N.; Nair, R.R.

A study is carried out to observe the angular dependence of backscattering strength at nodule area where grab sample and photographic data is available. Theoretical study along with the experimentally observed data shows that the backscattering...

An Ultrasonic Backscatter Instrument for Cancellous Bone Evaluation in Neonates

Directory of Open Access Journals (Sweden)

Chengcheng Liu

2015-09-01

Full Text Available Ultrasonic backscatter technique has shown promise as a noninvasive cancellous bone assessment tool. A novel ultrasonic backscatter bone diagnostic (UBBD instrument and an in vivo application for neonatal bone evaluation are introduced in this study. The UBBD provides several advantages, including noninvasiveness, non-ionizing radiation, portability, and simplicity. In this study, the backscatter signal could be measured within 5 s using the UBBD. Ultrasonic backscatter measurements were performed on 467 neonates (268 males and 199 females at the left calcaneus. The backscatter signal was measured at a central frequency of 3.5 MHz. The delay (T1 and duration (T2 of the backscatter signal of interest (SOI were varied, and the apparent integrated backscatter (AIB, frequency slope of apparent backscatter (FSAB, zero frequency intercept of apparent backscatter (FIAB, and spectral centroid shift (SCS were calculated. The results showed that the SOI selection had a direct influence on cancellous bone evaluation. The AIB and FIAB were positively correlated with the gestational age (|R| up to 0.45, P10 µs. Moderate positive correlations (|R| up to 0.45, P10 µs. The T2 mainly introduced fluctuations in the observed correlation coefficients. The moderate correlations observed with UBBD demonstrate the feasibility of using the backscatter signal to evaluate neonatal bone status. This study also proposes an explicit standard for in vivo SOI selection and neonatal cancellous bone assessment.

3D Backscatter Imaging System

Science.gov (United States)

Whitaker, Ross (Inventor); Turner, D. Clark (Inventor)

2016-01-01

Systems and methods for imaging an object using backscattered radiation are described. The imaging system comprises both a radiation source for irradiating an object that is rotationally movable about the object, and a detector for detecting backscattered radiation from the object that can be disposed on substantially the same side of the object as the source and which can be rotationally movable about the object. The detector can be separated into multiple detector segments with each segment having a single line of sight projection through the object and so detects radiation along that line of sight. Thus, each detector segment can isolate the desired component of the backscattered radiation. By moving independently of each other about the object, the source and detector can collect multiple images of the object at different angles of rotation and generate a three dimensional reconstruction of the object. Other embodiments are described.

The immunoassay handbook

National Research Council Canada - National Science Library

Wild, David (David G.)

2001-01-01

... importantly, enabling them to keep current on the basic theory behind immunoassay. Since the publication of the previous edition in 1994, the field has continued to evolve rapidly, and the need for a fully updated version of this book is now paramount. The second edition has been comprehensively updated and new chapters have been added to each section" [publisher's web site].

Evaluation of Six Different Immunoassays for Serum Thyrotropin

International Nuclear Information System (INIS)

Ma Donghong; Lu Hankui; Gao Yunchao; Ge Wenli; Xiong Jiang; Liu Qiaoping; Gu Qing

2010-01-01

To analyzes the discrepancy and association among six different thyrotropin (TSH) immunoassay methods and to study their impact on the clinical diagnoses of thyroid diseases, the 150 serum samples from three groups consisting of hyperthyroidism, hypothyroidism and healthy subjects, 50 samples in each group were included in this study. The serum TSH levels were measured simultaneously by radioimmunoassay (RIA), immunoradiometric assay (IRMA), three-type chemilumiminescence immunoassay (CLIA) and electrochemiluminescence immunoassay (ECLIA). The results showed that individual serum TSH level varied significantly from one assay to another. There was no correlation between TSH RIA and other five assays in groups of hyperthyroidism and healthy subjects(P>0.05). The correlations between TSH IRMA and four automatic assays in hyperthyroidism group were relatively low (r= 0.38∼0.41). However, among the four automatic assays, TSH levels were well correlated (r= 0.92∼0.99). For clinical diagnoses, TSH RIA alone was not useful in the differentiation of hyperthyroidism and normal subjects, and TSH IRMA was misleading in some hyperthyroidism. There were no significant differences for four TSH automatic immunoassays in differential diagnoses of thyroid diseases. (authors)

A Homogeneous Time-Resolved Fluorescence Immunoassay Method for the Measurement of Compound W.

Science.gov (United States)

Huang, Biao; Yu, Huixin; Bao, Jiandong; Zhang, Manda; Green, William L; Wu, Sing-Yung

2018-01-01

Using compound W (a 3,3'-diiodothyronine sulfate [T 2 S] immuno-crossreactive material)-specific polyclonal antibodies and homogeneous time-resolved fluorescence immunoassay assay techniques (AlphaLISA) to establish an indirect competitive compound W (ICW) quantitative detection method. Photosensitive particles (donor beads) coated with compound W or T 2 S and rabbit anti-W antibody were incubated with biotinylated goat anti-rabbit antibody. This constitutes a detection system with streptavidin-coated acceptor particle. We have optimized the test conditions and evaluated the detection performance. The sensitivity of the method was 5 pg/mL, and the detection range was 5 to 10 000 pg/mL. The intra-assay coefficient of variation averages W levels in extracts of maternal serum samples. This may have clinical application to screen congenital hypothyroidism in utero.

Interlinking backscatter, grain size and benthic community structure

Science.gov (United States)

McGonigle, Chris; Collier, Jenny S.

2014-06-01

The relationship between acoustic backscatter, sediment grain size and benthic community structure is examined using three different quantitative methods, covering image- and angular response-based approaches. Multibeam time-series backscatter (300 kHz) data acquired in 2008 off the coast of East Anglia (UK) are compared with grain size properties, macrofaunal abundance and biomass from 130 Hamon and 16 Clamshell grab samples. Three predictive methods are used: 1) image-based (mean backscatter intensity); 2) angular response-based (predicted mean grain size), and 3) image-based (1st principal component and classification) from Quester Tangent Corporation Multiview software. Relationships between grain size and backscatter are explored using linear regression. Differences in grain size and benthic community structure between acoustically defined groups are examined using ANOVA and PERMANOVA+. Results for the Hamon grab stations indicate significant correlations between measured mean grain size and mean backscatter intensity, angular response predicted mean grain size, and 1st principal component of QTC analysis (all p PERMANOVA for the Hamon abundance shows benthic community structure was significantly different between acoustic groups for all methods (p ≤ 0.001). Overall these results show considerable promise in that more than 60% of the variance in the mean grain size of the Clamshell grab samples can be explained by mean backscatter or acoustically-predicted grain size. These results show that there is significant predictive capacity for sediment characteristics from multibeam backscatter and that these acoustic classifications can have ecological validity.

Nanobody medicated immunoassay for ultrasensitive detection of cancer biomarker alpha-fetoprotein.

Science.gov (United States)

Chen, Jing; He, Qing-hua; Xu, Yang; Fu, Jin-heng; Li, Yan-ping; Tu, Zhui; Wang, Dan; Shu, Mei; Qiu, Yu-lou; Yang, Hong-wei; Liu, Yuan-yuan

2016-01-15

Immunoassay for cancer biomarkers plays an important role in cancer prevention and early diagnosis. To the development of immunoassay, the quality and stability of applied antibody is one of the key points to obtain reliability and high sensitivity for immunoassay. The main purpose of this study was to develop a novel immunoassay for ultrasensitive detection of cancer biomarker alpha-fetoprotein (AFP) based on nanobody against AFP. Two nanobodies which bind to AFP were selected from a phage display nanobody library by biopanning strategy. The prepared nanobodies are clonable, thermally stable and applied in both sandwich enzyme linked immunoassay (ELISA) and immuno-PCR assay for ultrasensitive detection of AFP. The limit detection of sandwich ELISA setup with optimized nanobodies was 0.48ng mL(-1), and the half of saturation concentration (SC50) value was 6.68±0.56ng mL(-1). These nanobodies were also used to develop an immuno-PCR assay for ultrasensitive detection of AFP, its limit detection values was 0.005ng mL(-1), and the linear range was 0.01-10,000ng mL(-1). These established immunoassays based on nanobodies were highly specific to AFP and with negligible cross reactivity with other tested caner biomarkers. Furthermore, this novel concept of nanobodies mediated immunoassay may provide potential applications in a general method for the ultrasensitive detection of various cancer biomarkers. Copyright © 2015 Elsevier B.V. All rights reserved.

The physical-optics approximation and its application to light backscattering by hexagonal ice crystals

International Nuclear Information System (INIS)

Borovoi, A.; Konoshonkin, A.; Kustova, N.

2014-01-01

The physical-optics approximation in the problem of light scattering by large particles is so defined that it includes the classical physical optics concerning the problem of light penetration through a large aperture in an opaque screen. In the second part of the paper, the problem of light backscattering by quasi-horizontally oriented atmospheric ice crystals is considered where conformity between the physical-optics and geometric-optics approximations is discussed. The differential scattering cross section as well as the polarization elements of the Mueller matrix for quasi-horizontally oriented hexagonal ice plates has been calculated in the physical-optics approximation for the case of vertically pointing lidars. - Highlights: • The physical-optics Mueller matrix is a smoothed geometric-optics counterpart. • Backscatter by partially oriented hexagonal ice plates has been calculated. • Depolarization ratio for partially oriented hexagonal ice plates is negligible

X-ray backscatter imaging with a spiral scanner

International Nuclear Information System (INIS)

Bossi, R.H.; Cline, J.L.; Friddell, K.D.

1989-01-01

X-ray backscatter imaging allows radiographic inspections to be performed with access to only one side of the object. A collimated beam of radiation striking an object will scatter x-rays by Compton scatter and x-ray fluorescence. A detector located on the source side of the part will measure the backscatter signal. By plotting signal strength as gray scale intensity vs. beam position on the object, an image of the object can be constructed. A novel approach to the motion of the collimated incident beam is a spiral scanner. The spiral scanner approach, described in this paper, can image an area of an object without the synchronized motion of the object or detector, required by other backscatter imaging techniques. X-ray backscatter is particularly useful for flaw detection in light element materials such as composites. The ease of operation and the ability to operate non-contact from one side of an object make x-ray backscatter imaging of increasing interest to industrial inspection problems

On line ultrasonic integrated backscatter

International Nuclear Information System (INIS)

Landini, L.; Picano, E.; Mazzarisi, A.; Santarelli, F.; Benassi, A.; De Pieri, G.

1988-01-01

A new equipment for on-line evaluation of index based on two-dimensional integrated backscatter from ultrasonic images is described. The new equipment is fully integrated into a B-mode ultrasonic apparatus which provides a simultaneous display of conventional information together with parameters of tissue characterization. The system has been tested with a backscattering model of microbubbles in polysaccharide solution, characterized by a physiological exponential time decay. An exponential fitting to the experimental data was performed which yielded r=0.95

Backscattering at a pulsed neutron source, the MUSICAL instrument

International Nuclear Information System (INIS)

Alefeld, B.

1995-01-01

In the first part the principles of the neutron backscattering method are described and some simple considerations about the energy resolution and the intensity are presented. A prototype of a backscattering instrument, the first Juelich instrument, is explained in some detail and a representative measurement is shown which was performed on the backscattering instrument IN10 at the ILL in Grenoble. In the second part a backscattering instrument designed for a pulsed neutron source is proposed. It is shown that a rather simple modification, which consists in the replacement of the Doppler drive of the conventional backscattering instrument by a multi silicon monochromator crystal (MUSICAL) leads to a very effective instrument, benefitting from the peak flux of the pulsed source. ((orig.))

Super-virtual Interferometric Separation and Enhancement of Back-scattered Surface Waves

KAUST Repository

Guo, Bowen

2015-08-19

Back-scattered surface waves can be migrated to detect near-surface reflectors with steep dips. A robust surface-wave migration requires the prior separation of the back-scattered surface-wave events from the data. This separation is often difficult to implement because the back-scattered surface waves are masked by the incident surface waves. We mitigate this problem by using a super-virtual interferometric method to enhance and separate the back-scattered surface waves. The key idea is to calculate the virtual back-scattered surface waves by stacking the resulting virtual correlated and convolved traces associated with the incident and back-scattered waves. Stacking the virtual back-scattered surface waves improves their signal-to-noise ratio and separates the back-scattered surface-waves from the incident field. Both synthetic and field data results validate the robustness of this method.

Effective gasoline site assessment using the D TECH trademark BTEX immunoassay

International Nuclear Information System (INIS)

Hudak, R.T.; Melby, J.M.; Stave, J.W.

1994-01-01

The application of immunoassay to environmental testing can greatly aid in assessing sites for various contaminants including PCB, TNT, RDX, PAH and BTEX. Immunoassay offers several benefits to site assessment: it is accurate, reproducible and relatively inexpensive compared to instrumental analysis. In addition, because of its ease-of-use, this technique is ideal for the field and requires minimal user training. To demonstrate not only the effectiveness of the BTEX immunoassay, but also the reliability of the field results, a gasoline contaminated site was assessed comparing the BTEX immunoassay to gas chromatography. All sampling and site related activities were executed in accordance to the USEPA SW-846 guidelines. Three (3) analyses were performed on each sample. One immunoassay analysis was performed in the field by an individual who received two (2) hours of training prior to the start of the study. A technician familiar with the immunoassay ran the second analysis in a laboratory. Finally, an independent GC laboratory certified for BTEX method 8020 and 602 performed the GC analyses. One hundred one (101) samples were analyzed: thirty-nine (39) samples were water, the other sixty-two (62) were soils ranging from clay to silt. The results and costs of the methods are compared

Remote sensing of particle backscattering in Chesapeake Bay: a 6-year SeaWiFS retrospective view

Science.gov (United States)

Zawada, D.G.; Hu, C.; Clayton, T.; Chen, Z.; Brock, J.C.; Muller-Karger, F. E.

2007-01-01

Traditional field techniques to monitor water quality in large estuaries, such as boat-based surveys and autonomous moored sensors, generally provide limited spatial coverage. Satellite imagery potentially can be used to address both of these limitations. Here, we show that satellite-based observations are useful for inferring total-suspended-solids (TSS) concentrations in estuarine areas. A spectra-matching optimization algorithm was used to estimate the particle backscattering coefficient at 400 nm, bbp(400), in Chesapeake Bay from Sea-viewing Wide-Field-of-view Sensor (SeaWiFS) satellite imagery. These estimated values of bbp(400) were compared to in situ measurements of TSS for the study period of September 1997–December 2003. Contemporaneous SeaWiFS bbp(400) values and TSS concentrations were positively correlated (N = 340, r2 = 0.4, P bp(400) values served as a reasonable first-order approximation for synoptically mapping TSS. Overall, large-scale patterns of SeaWiFS bbp(400) appeared to be consistent with expectations based on field observations and historical reports of TSS. Monthly averages indicated that SeaWiFS bbp(400) was typically largest in winter (>0.049 m−1, November–February) and smallest in summer (−1, June–August), regardless of the amount of riverine discharge to the bay. The study period also included Hurricanes Floyd and Isabel, which caused large-scale turbidity events and changes in the water quality of the bay. These results demonstrate that this technique can provide frequent synoptic assessments of suspended solids concentrations in Chesapeake Bay and other coastal regions.

Evidence of a tropospheric aerosol backscatter background mode

Science.gov (United States)

Rothermel, Jeffry; Bowdle, David A.; Vaughan, J. Michael; Post, Madison J.

1989-01-01

Vertical profiles of atmospheric aerosol backscatter coefficients at 10.6 microns obtained with airborne and ground-based lidar are compared. Both sets of profiles show a high frequency of occurrence of low backscatter over a limited range of values in the middle and upper troposphere. It is suggested that this narrow range indicates a ubiquitous background mode for atmospheric backscatter around the globe. Implications of such a mode for global scale aerosol models and for the design of satellite-borne lidar-based sensors are discussed.

Preliminary backscatter results from the hydrosweep multibeam system

Digital Repository Service at National Institute of Oceanography (India)

Hagen, R.A.; Chakraborty, B.; Schenke, H.W.

of Oceanography to convert the measured electrical energy into acoustic backscatter energy. This conversion includes corrections for the position, slope, and area of the scattering surface. In this paper we present backscatter data from several areas surveyed...

Detection of narcotics with an immunoassay film badge

International Nuclear Information System (INIS)

Lukens, H.R.

1993-01-01

Efficient personnel performance, a major requirement for a safe nuclear industry, is jeopardized where personnel use narcotics. However, detection of narcotics at nuclear plants is a challenge. The unique specificity and sensitivity of an immunoassay has been implemented in the form of a small, dry immunoassay film badge (IFB) for the detection of vapors emitted by narcotics. The device is suitable as an area monitor, and its characteristics are suitable for use as a breath monitor for the detection of drug use

Transport equation theory of electron backscattering and x-ray production

International Nuclear Information System (INIS)

Fathers, D.J.; Rez, P.

1978-02-01

A transport equation theory of electron backscattering and x ray production is derived and applied to energy dissipation of 30-KeV electrons for copper as a function of depth and to the energy distribution of backscattered electrons for copper, aluminum, and gold. These results are plotted and compared with experiment. Plots for variations of backscattering with atomic number and with angle of incidence, and polar plots of backscattering for 30-keV electrons at normal incidence are also presented. 10 references, seven figures

Development of national immunoassay reagent programmes

International Nuclear Information System (INIS)

Sufi, S.B.; Micallef, J.V.; Ahsan, R.; Goncharov, N.P.

1992-01-01

Despite the existence of networks of fully equipped laboratories with well-trained staff, the availability of immunodiagnostic services in developing countries is often limited by the high cost of imported kits. There are a number of ways of tackling this problem, ranging from bulk purchase of kits or reagents to local development and production of assay systems. Argentina/Chile, China, Cuba/Mexico, and Thailand are amongst the countries which have established local immunoassay reagent programmes to manufacture low cost, high quality immunoassay reagents. Kits from these projects are now beginning to become available, and it is hoped that they will promote national diagnostic services and research, as well as stimulating the development of reagent programmes for other analytes. (author). 4 refs, 1 tab

Estimating sub-surface dispersed oil concentration using acoustic backscatter response.

Science.gov (United States)

Fuller, Christopher B; Bonner, James S; Islam, Mohammad S; Page, Cheryl; Ojo, Temitope; Kirkey, William

2013-05-15

The recent Deepwater Horizon disaster resulted in a dispersed oil plume at an approximate depth of 1000 m. Several methods were used to characterize this plume with respect to concentration and spatial extent including surface supported sampling and autonomous underwater vehicles with in situ instrument payloads. Additionally, echo sounders were used to track the plume location, demonstrating the potential for remote detection using acoustic backscatter (ABS). This study evaluated use of an Acoustic Doppler Current Profiler (ADCP) to quantitatively detect oil-droplet suspensions from the ABS response in a controlled laboratory setting. Results from this study showed log-linear ABS responses to oil-droplet volume concentration. However, the inability to reproduce ABS response factors suggests the difficultly in developing meaningful calibration factors for quantitative field analysis. Evaluation of theoretical ABS intensity derived from the particle size distribution provided insight regarding method sensitivity in the presence of interfering ambient particles. Copyright © 2013 Elsevier Ltd. All rights reserved.

Control of (pre-analytical aspects in immunoassay measurements of metabolic hormones in rodents

Directory of Open Access Journals (Sweden)

Maximilian Bielohuby

2018-04-01

Full Text Available The measurement of circulating hormones by immunoassay remains a cornerstone in preclinical endocrine research. For scientists conducting and interpreting immunoassay measurements of rodent samples, the paramount aim usually is to obtain reliable and meaningful measurement data in order to draw conclusions on biological processes. However, the biological variability between samples is not the only variable affecting the readout of an immunoassay measurement and a considerable amount of unwanted or unintended variability can be quickly introduced during the pre-analytical and analytical phase. This review aims to increase the awareness for the factors ‘pre-analytical’ and ‘analytical’ variability particularly in the context of immunoassay measurement of circulating metabolic hormones in rodent samples. In addition, guidance is provided how to gain control over these variables and how to avoid common pitfalls associated with sample collection, processing, storage and measurement. Furthermore, recommendations are given on how to perform a basic validation of novel single and multiplex immunoassays for the measurement of metabolic hormones in rodents. Finally, practical examples from immunoassay measurements of plasma insulin in mice address the factors ‘sampling site and inhalation anesthesia’ as frequent sources of introducing an unwanted variability during the pre-analytical phase. The knowledge about the influence of both types of variability on the immunoassay measurement of circulating hormones as well as strategies to control these variables are crucial, on the one hand, for planning and realization of metabolic rodent studies and, on the other hand, for the generation and interpretation of meaningful immunoassay data from rodent samples.

Strong Localization in Disordered Media: Analysis of the Backscattering Cone

KAUST Repository

Delgado, Edgar

2012-06-01

A very interesting effect in light propagation through a disordered system is Anderson localization of light, this phenomenon emerges as the result of multiple scattering of waves by electric inhomogeneities like spatial variations of index of refraction; as the amount of scattering is increased, light propagation is converted from quasi-diffusive to exponentially localized, with photons confined in a limited spatial region characterized by a fundamental quantity known as localization length. Light localization is strongly related to another interference phenomenon emerged from the multiple scattering effect: the coherent backscattering effect. In multiple scattering of waves, in fact, coherence is preserved in the backscattering direction and produces a reinforcement of the field flux originating an observable peak in the backscattered intensity, known as backscattering cone. The study of this peak provide quantitative information about the transport properties of light in the material. In this thesis we report a complete FDTD ab-initio study of light localization and coherent backscattering. In particular, we consider a supercontinuum pulse impinging on a sample composed of randomly positioned scatterers. We study coherent backscattering by averaging over several realizations of the sample properties. We study then the coherent backscattering cone properties as the relative permittivity of the sample is changed, relating the latter with the light localization inside the sample. We demonstrate important relationships between the width of the backscattering cone and the localization length, which shows a linear proportionality in the strong localization regime.

Relation between Raman backscattering from droplets and bulk water: Effect of refractive index dispersion

Science.gov (United States)

Plakhotnik, Taras; Reichardt, Jens

2018-03-01

A theoretical framework is presented that permits investigations of the relation between inelastic backscattering from microparticles and bulk samples of Raman-active materials. It is based on the Lorentz reciprocity theorem and no fundamental restrictions concerning the microparticle shape apply. The approach provides a simple and intuitive explanation for the enhancement of the differential backscattering cross-section in particles in comparison to bulk. The enhancement factor for scattering of water droplets in the diameter range from 0 to 60 μm (vitally important for the a priori measurement of liquid water content of warm clouds with spectroscopic Raman lidars) is about a factor of 1.2-1.6 larger (depending on the size of the sphere) than an earlier study has shown. The numerical calculations are extended to 1000 μm and demonstrate that dispersion of the refractive index of water becomes an important factor for spheres larger than 100 μm. The physics of the oscillatory phenomena predicted by the simulations is explained.

Backscattered electron imaging at low emerging angles: A physical approach to contrast in LVSEM

Energy Technology Data Exchange (ETDEWEB)

Cazaux, J., E-mail: jacques.cazaux@univ-reims.fr [LISM, EA 4695 Faculty of Sciences, BP 1039, 51687 Reims Cedex 2 (France); Kuwano, N. [Malaysia–Japan International Institute of Technology, Universiti Teknologi Malaysia, Jalan Semarak, 54100 Kuala Lumpur (Malaysia); Sato, K. [Steel Research Laboratory, JFE Steel Corporation, 1 Kawasaki-cho, Chuo-ku, Chiba 260-0835 (Japan)

2013-12-15

Due to the influence of refraction effects on the escape probability of the Back-Scattered Electrons (BSE), an expression of the fraction of these BSE is given as a function of the beam energy, E°, and emission angle (with respect to the normal) α. It has been shown that these effects are very sensitive to a local change of the work function in particular for low emerging angles. This sensitivity suggests a new type of contrast in Low Voltage Scanning Electron Microscopy (LVSEM for E°<2 keV): the work function contrast. Involving the change of ϕ with crystalline orientation, this possibility is supported by a new interpretation of a few published images. Some other correlated contrasts are also suggested. These are topographical contrasts or contrasts due to subsurface particles and cracks. Practical considerations of the detection system and its optimization are indicated. - Highlights: • Refraction effects experienced by Back-Scattered Electrons at sample/vacuum interfaces are evaluated as a function of energy and angles. • Sensitive to local work function changes with crystalline orientation these effects concern mainly keV-electrons at low emerging angles. • A new type of contrast in SEM is thus deduced and illustrated. • Some other correlated contrasts, topographical contrasts or contrasts due to subsurface particles and cracks are also suggested.

Effect of the cortex on ultrasonic backscatter measurements of cancellous bone

International Nuclear Information System (INIS)

Hoffmeister, Brent K; Holt, Andrew P; Kaste, Sue C

2011-01-01

Ultrasonic backscatter techniques offer a promising new approach for detecting changes in bone caused by osteoporosis. However, several challenges impede clinical implementation of backscatter techniques. This study examines how the dense outer surface of bone (the cortex) affects backscatter measurements of interior regions of porous (cancellous) bone tissue. Fifty-two specimens of bone were prepared from 13 human femoral heads so that the same region of cancellous bone could be ultrasonically interrogated through the cortex or along directions that avoided the cortex. Backscatter signals were analyzed over a frequency range of 0.8-3.0 MHz to determine two ultrasonic parameters: apparent integrated backscatter (AIB) and frequency slope of apparent backscatter (FSAB). The term 'apparent' means that the parameters are sensitive to the frequency-dependent effects of diffraction and attenuation. Significant (p < 0.001) changes in AIB and FSAB indicated that measurements through the cortex decreased the apparent backscattered power and increased the frequency dependence of the power. However, the cortex did not affect the correlation of AIB and FSAB with the x-ray bone mineral density of the specimens. This suggests that results from many previous in vitro backscatter studies of specimens of purely cancellous bone may be extrapolated with greater confidence to in vivo conditions.

Effect of the cortex on ultrasonic backscatter measurements of cancellous bone

Energy Technology Data Exchange (ETDEWEB)

Hoffmeister, Brent K; Holt, Andrew P [Department of Physics, Rhodes College, Memphis, TN (United States); Kaste, Sue C, E-mail: hoffmeister@rhodes.edu [Department of Diagnostic Imaging, St Jude Children' s Research Hospital, Memphis, TN (United States)

2011-10-07

Ultrasonic backscatter techniques offer a promising new approach for detecting changes in bone caused by osteoporosis. However, several challenges impede clinical implementation of backscatter techniques. This study examines how the dense outer surface of bone (the cortex) affects backscatter measurements of interior regions of porous (cancellous) bone tissue. Fifty-two specimens of bone were prepared from 13 human femoral heads so that the same region of cancellous bone could be ultrasonically interrogated through the cortex or along directions that avoided the cortex. Backscatter signals were analyzed over a frequency range of 0.8-3.0 MHz to determine two ultrasonic parameters: apparent integrated backscatter (AIB) and frequency slope of apparent backscatter (FSAB). The term 'apparent' means that the parameters are sensitive to the frequency-dependent effects of diffraction and attenuation. Significant (p < 0.001) changes in AIB and FSAB indicated that measurements through the cortex decreased the apparent backscattered power and increased the frequency dependence of the power. However, the cortex did not affect the correlation of AIB and FSAB with the x-ray bone mineral density of the specimens. This suggests that results from many previous in vitro backscatter studies of specimens of purely cancellous bone may be extrapolated with greater confidence to in vivo conditions.

Label-Free Electrochemical Immunoassay for C-Reactive Protein

Directory of Open Access Journals (Sweden)

Madasamy Thangamuthu

2018-03-01

Full Text Available C-reactive protein (CRP is one of the most expressed proteins in blood during acute phase inflammation, and its minute level increase has also been recognized for the clinical diagnosis of cardio vascular diseases. Unfortunately, the available commercial immunoassays are labour intensive, require large sample volumes, and have practical limitations, such as low stability and high production costs. Hence, we have developed a simple, cost effective, and label-free electrochemical immunoassay for the measurement of CRP in a drop of serum sample using an immunosensor strip made up of a screen printed carbon electrode (SPE modified with anti-CRP functionalized gold nanoparticles (AuNPs. The measurement relies on the decrease of the oxidation current of the redox indicator Fe3+/Fe2+, resulting from the immunoreaction between CRP and anti-CRP. Under optimal conditions, the present immunoassay measures CRP in a linear range from 0.4–200 nM (0.047–23.6 µg mL−1, with a detection limit of 0.15 nM (17 ng mL−1, S/N = 3 and sensitivity of 90.7 nA nM−1, in addition to a good reproducibility and storage stability. The analytical applicability of the presented immunoassay is verified by CRP measurements in human blood serum samples. This work provides the basis for a low-priced, safe, and easy-to-use point-of-care immunosensor assay to measure CRP at clinically relevant concentrations.

Micromotor-based lab-on-chip immunoassays

Science.gov (United States)

García, Miguel; Orozco, Jahir; Guix, Maria; Gao, Wei; Sattayasamitsathit, Sirilak; Escarpa, Alberto; Merkoçi, Arben; Wang, Joseph

2013-01-01

Here we describe the first example of using self-propelled antibody-functionalized synthetic catalytic microengines for capturing and transporting target proteins between the different reservoirs of a lab-on-a-chip (LOC) device. A new catalytic polymer/Ni/Pt microtube engine, containing carboxy moieties on its mixed poly(3,4-ethylenedioxythiophene) (PEDOT)/COOH-PEDOT polymeric outermost layer, is further functionalized with the antibody receptor to selectively recognize and capture the target protein. The new motor-based microchip immunoassay operations are carried out without any bulk fluid flow, replacing the common washing steps in antibody-based protein bioassays with the active transport of the captured protein throughout the different reservoirs, where each step of the immunoassay takes place. A first microchip format involving an `on-the-fly' double-antibody sandwich assay (DASA) is used for demonstrating the selective capture of the target protein, in the presence of excess of non-target proteins. A secondary antibody tagged with a polymeric-sphere tracer allows the direct visualization of the binding events. In a second approach the immuno-nanomotor captures and transports the microsphere-tagged antigen through a microchannel network. An anti-protein-A modified microengine is finally used to demonstrate the selective capture, transport and convenient label-free optical detection of a Staphylococcus aureus target bacteria (containing proteinA in its cell wall) in the presence of a large excess of non-target (Saccharomyces cerevisiae) cells. The resulting nanomotor-based microchip immunoassay offers considerable potential for diverse applications in clinical diagnostics, environmental and security monitoring fields.Here we describe the first example of using self-propelled antibody-functionalized synthetic catalytic microengines for capturing and transporting target proteins between the different reservoirs of a lab-on-a-chip (LOC) device. A new catalytic

Quantitative characterization of abyssal seafloor with transit multibeam backscatter data

Science.gov (United States)

Pockalny, R. A.; Ferrini, V. L.

2014-12-01

The expanding volume of deep-water multibeam echosounder data provides emerging opportunities for the improved characterization of the abyssal seafloor. Nearly 500 cruises criss-cross the oceans with modern wide-swath multibeam systems, and these cruise tracks have imaged a variety of morphologic, tectonic and magmatic environments. The qualitative analysis of the seafloor backscatter data strongly suggests a local and regional variability that correlates with sediment thickness, sediment type and/or depositional environment. We present our initial attempts to develop a method that quantifies this observed seafloor backscatter variability and to explore the causes and potential implications of this variability. Our approach is rooted in the Angular Range Analysis methodology, which utilizes changes in backscatter amplitude observed as a function of grazing angle, to characterize the seafloor. The primary difference in our approach is that we do not invert for geo-acoustical parameters, but rather explores empirical relationships between geological observations and stacked slope and y-intercept values. In addition, we also include the mean and the variance of detrended backscatter measurements. Our initial results indicate intriguing relationships between backscatter parameters and the CaCO3 content of surface sediments. Seafloor regions reported to have high manganese nodule concentrations also tend to have characteristic trends in backscatter parameters. We will present these regional correlations as well as some preliminary statistical analyses of the backscatter parameters and key environmental factors.

Determining total thyroxine with the aid of the homogeneous enzyme immunoassay

International Nuclear Information System (INIS)

Vogt, W.

1978-01-01

Total thyrozine values obtained with the aid of the homogeneous enzyme immunoassay are compared with those delivered by the RIA and the efficiency of the EMIT technique is evaluated. Some results obtained via Enzymun-T4, a heterologous enzyme immunoassay, are also given. (VJ) 891 VJ [de

Lidar- and balloon-borne particle counter comparisons following recent volcanic eruptions

Science.gov (United States)

Hofmann, D. J.; Rosen, J. M.; Reiter, R.; Jager, H.

1983-01-01

Balloon-borne particle counter measurements at Laramie, Wyoming (41 deg N) are used to calculate the expected lidar backscatter at 0.694 micron wavelength from July 1979 to February 1982, a period which included at least four detectable perturbations of the stratospheric aerosol layer due to volcanic eruptions. These calculations are compared with lidar measurements conducted at Garmisch-Partenkirchen (47.5 deg N) during the same period. While the agreement is generally good using only the main mode in the particle size distribution (radius about 0.07 micron) during approximately the first 6 months following a major volcanic eruption, a measured secondary mode near 1 micron radius, when included, improves the agreement. Calculations of the expected backscatter at 25-30 km reveal that substantial number of particles diffuse into this high altitude region about 7 months after a major eruption, and these particles should be taken into account when normalizing lidar at these altitudes.

Time evolution analysis of the electron distribution in Thomson/Compton back-scattering

International Nuclear Information System (INIS)

Petrillo, V.; Bacci, A.; Curatolo, C.; Maroli, C.; Serafini, L.; Rossi, A. R.

2013-01-01

We present the time evolution of the energy distribution of a relativistic electron beam after the Compton back-scattering with a counter-propagating laser field, performed in the framework of the Quantum Electrodynamics, by means of the code CAIN. As the correct angular distribution of the spontaneous emission is accounted, the main effect is the formation of few stripes, followed by the diffusion of the more energetic particles toward lower values in the longitudinal phase space. The Chapman-Kolmogorov master equation gives results in striking agreement with the numerical ones. An experiment on the Thomson source at SPARC-LAB is proposed

Ocean subsurface particulate backscatter estimation from CALIPSO spaceborne lidar measurements

Science.gov (United States)

Chen, Peng; Pan, Delu; Wang, Tianyu; Mao, Zhihua

2017-10-01

A method for ocean subsurface particulate backscatter estimation from the Cloud-Aerosol Lidar with Orthogonal Polarization (CALIOP) on the Cloud-Aerosol Lidar and Infrared Pathfinder Satellite Observation (CALIPSO) satellite was demonstrated. The effects of the CALIOP receiver's transient response on the attenuated backscatter profile were first removed. The two-way transmittance of the overlying atmosphere was then estimated as the ratio of the measured ocean surface attenuated backscatter to the theoretical value computed from wind driven wave slope variance. Finally, particulate backscatter was estimated from the depolarization ratio as the ratio of the column-integrated cross-polarized and co-polarized channels. Statistical results show that the derived particulate backscatter by the method based on CALIOP data agree reasonably well with chlorophyll-a concentration using MODIS data. It indicates a potential use of space-borne lidar to estimate global primary productivity and particulate carbon stock.

Identification of major backscattering sources in trees and shrubs at 10 GHz

Science.gov (United States)

Zoughi, R.; Wu, L. K.; Moore, R. K.

1986-01-01

A short-range very-fine-resolution FM-CW radar scatterometer has been used to identify the primary contributors to 10-GHz radar backscatter from pine, pin oak, American sycamore and sugar maple trees, and from creeping juniper shrubs. This system provided a range resolution of 11 cm and gave a 16-cm diameter illumination area at the target range of about 4 m. For a pine tree, the needles caused the strongest backscatter as well as the strongest attenuation in the radar signal. Cones, although insignificant contributors to the total backscatter, were more important for backscattering than for attenuation. For the rest of the trees, leaves were the strongest cause of backscattering and attenuation. However, in the absence of leaves, the petioles, small twigs, and branches gave relatively strong backscatter. For American sycamore and sugar maple trees, the fruits did not affect the total backscatter unless they were packed in clusters. For creeping juniper the backscattered energy and attenuation in the radar signal were mainly due to the top two layers of the evergreen scales. The contribution of the tree trunks was not determined.

Rational design of Raman-labeled nanoparticles for a dual-modality, light scattering immunoassay on a polystyrene substrate.

Science.gov (United States)

Israelsen, Nathan D; Wooley, Donald; Hanson, Cynthia; Vargis, Elizabeth

2016-01-01

Surface-enhanced Raman scattering (SERS) is a powerful light scattering technique that can be used for sensitive immunoassay development and cell labeling. A major obstacle to using SERS is the complexity of fabricating SERS probes since they require nanoscale characterization and optical uniformity. The light scattering response of SERS probes may also be modulated by the substrate used for SERS analysis. A typical SERS substrate such as quartz can be expensive. Polystyrene is a cheaper substrate option but can decrease the SERS response due to interfering Raman emission peaks and high background fluorescence. The goal of this research is to develop an optimized process for fabricating Raman-labeled nanoparticles for a SERS-based immunoassay on a polystyrene substrate. We have developed a method for fabricating SERS nanoparticle probes for use in a light scattering immunoassay on a polystyrene substrate. The light scattering profile of both spherical gold nanoparticle and gold nanorod SERS probes were characterized using Raman spectroscopy and optical absorbance spectroscopy. The effects of substrate interference and autofluorescence were reduced by selecting a Raman reporter with a strong light scattering response in a spectral region where interfering substrate emission peaks are minimized. Both spherical gold nanoparticles and gold nanorods SERS probes used in the immunoassay were detected at labeling concentrations in the low pM range. This analytical sensitivity falls within the typical dynamic range for direct labeling of cell-surface biomarkers using SERS probes. SERS nanoparticle probes were fabricated to produce a strong light scattering signal despite substrate interference. The optical extinction and inelastic light scattering of these probes was detected by optical absorbance spectroscopy and Raman spectroscopy, respectively. This immunoassay demonstrates the feasibility of analyzing strongly enhanced Raman signals on polystyrene, which is an

BATS - Backscattering And Time-of-flight Spectrometer

International Nuclear Information System (INIS)

Van Eijck, L.; Seydel, T.; Frick, B.; Schober, H.

2011-01-01

The new backscattering spectrometer IN16b will go into commissioning end 2011, providing in its final state about ten times higher count rate than its predecessor, IN16. Here we propose to increase its dynamic range by a factor of 7 with the TOF mode extension, BATS. This will make IN16b the leading high resolution backscattering spectrometer for incoherent quasi-elastic and inelastic neutron scattering; it will be competitive to the coarser resolution inverted geometry backscattering spectrometers that are being brought online at spallation sources. The increased dynamic range will extend the scope of science addressed on IN16b, generating considerable potential in fields such as the hydrogen economy (proton conduction, fuel cells, hydrogen storage), soft matter, biology and nano-science (nano-scale confinement, functionalized polymers). Such a large impact can be achieved using only a moderate investment. (authors)

Highly sensitive immunoassay based on E. coli with autodisplayed Z-domain

International Nuclear Information System (INIS)

Jose, Joachim; Park, Min; Pyun, Jae-Chul

2010-01-01

The Z-domain of protein A has been known to bind specifically to the F c region of antibodies (IgGs). In this work, the Z-domain of protein A was expressed on the outer membrane of Escherichia coli by using 'Autodisplay' technology as a fusion protein of autotransport domain. The E. coli with autodisplayed Z-domain was applied to the sandwich-type immunoassay as a solid-support of detection-antibodies against a target analyte. For the feasibility demonstration of the E. coli based immunoassay, C-reactive protein (CRP) assay was carried out by using E. coli with autodisplayed Z-domain. The limit of detection (LOD) and binding capacity of the E. coli based immunoassay were estimated to be far more sensitive than the conventional ELISA. Such a far higher sensitivity of E. coli based immunoassay than conventional ELISA was explained by the orientation control of immobilized antibodies and the mobility of E. coli in assay matrix. From the test results of 45 rheumatoid arthritis (RA) patients' serum and 15 healthy samples, a cut-off value was established to have optimal sensitivity and selectivity values for RA. The CRP test result of each individual sample was compared with ELISA which is the reference method for RA diagnosis. From this work, the E. coli with Z-domain was proved to be feasible for the medical diagnosis based on sandwich-type immunoassay.

Multiplex Immunoassay Profiling of Hormones Involved in Metabolic Regulation.

Science.gov (United States)

Stephen, Laurie; Guest, Paul C

2018-01-01

Multiplex immunoassays are used for rapid profiling of biomarker proteins and small molecules in biological fluids. The advantages over single immunoassays include lower sample consumption, cost, and labor. This chapter details a protocol to develop a 5-plex assay for glucagon-like peptide 1, growth hormone, insulin, leptin, and thyroid-stimulating hormone on the Luminex ® platform. The results of the analysis of insulin in normal control subjects are given due to the important role of this hormone in nutritional programming diseases.

Concentration Measurements of Suspended Load using ADV with Influence of the Particle Size

Science.gov (United States)

Schwarzwälder, Kordula

2017-04-01

ADV backscatter data can be used under certain conditions to gain information about the concentrations of suspended loads. This was shown in many studies before (Fugate and Friedrichs 2002; Chanson et al 2008; Ha et al. 2009). This paper reports on a pre-study to investigate the influence of particle size on concentration measurements for suspended sediment load with ADV. The study was conducted in a flume in the Oskar-von-Miller-Institute using fresh water from a river including the natural suspended load. The ADV used in the experiments was a Vectrino Profiler (Nortek). In addition water samples were taken for TSS and TOC. For the measurements a surge was generated in the flume to ensure that also particles of larger size will be present in the water phase. The measurements and samples were taken during the whole surge event. Therefore we were able to find a good correlation between the backscatter data of the ADV and the TSS as well as TOC results. For the decreasing part of the flow event the concentration of TOC in the suspended load of the water phase is decreasing much slower than the TSS and results in a damped decrease of the backscatter values. This means that the results for concentration measurements might be slightly influenced by the size of the particles. Further evaluations of measurements conducted with a LISST SL (Sequoia) will be investigated to show the trend of the particle sizes during this process and fortify this result. David C. Fugate, Carl T. Friedrichs, Determining concentration and fall velocity of estuarine particle populations using ADV, OBS and LISST, Continental Shelf Research, Volume 22, Issues 11-13, 2002 H.K. Ha, W.-Y. Hsu, J.P.-Y. Maa, Y.Y. Shao, C.W. Holland, Using ADV backscatter strength for measuring suspended cohesive sediment concentration, Continental Shelf Research, Volume 29, Issue 10, 2009 Hubert Chanson, Maiko Takeuchi, Mark Trevethan, Using turbidity and acoustic backscatter intensity as surrogate measures of

Multi-beam backscatter image data processing techniques employed to EM 1002 system

Digital Repository Service at National Institute of Oceanography (India)

Fernandes, W.A.; Chakraborty, B.

to compensate outer-beam backscatter strength data in such a way that the effect of angular backscatter strength is removed. In this work we have developed backscatter data processing techniques for EM1002 multi-beam system...

Potential applications of immunoassays in studies of flatfish recruitment

Science.gov (United States)

Feller, Robert J.

The fisheries recruitment-stock problem, a lack of correlation between measures of reproductive output of the parent stock and recruitment to the fishery, has several potential biotic and abiotic causes. Immunoassays may be useful in examining several aspects of this and several other problems in flatfish ecology: stock identification, parasitism and disease, and trophic interactions. Given stage-specific antisera capable of recognozing antigenic moieties of, for instance, eggs, larvae, or newly-settled juveniles, it is possible to screen stomach contents of many putative predators ( e.g., shrimp or crabs) rapidly for the presence and amounts of platfish prey. This trophic application of immunological methods has great promise for measuring loss of potential recruits to predation. All immunoassays are limited by the quality of antisera used and the researcher's ability to interpret quantitative data in an ecologically meaningful way. Key references for applications of immunoassays in fish-related questions are provided with recommendations for their utilization.

Negative interference by rheumatoid factor in alpha-fetoprotein chemiluminescent microparticle immunoassay.

Science.gov (United States)

Wang, Hui; Bi, Xiaohui; Xu, Lei; Li, Yirong

2017-01-01

Background Rheumatoid factor causes positive interference in multiple immunoassays. Recently, negative interference has also been found in immunoassays in the presence of rheumatoid factor. The chemiluminescent microparticle immunoassay is widely used to determine serum alpha-fetoprotein. However, it is not clear whether the presence of rheumatoid factor in the serum causes interference in the chemiluminescent microparticle immunoassay of alpha-fetoprotein. Methods Serum alpha-fetoprotein was determined using the ARCHITECT alpha-fetoprotein assay. The estimation of alpha-fetoprotein recovery was carried out in samples prepared by diluting high-concentration alpha-fetoprotein serum with rheumatoid factor-positive or rheumatoid factor-negative serum. Paramagnetic microparticles coated with hepatitis B surface antigen-anti-HBs complexes were used to remove rheumatoid factor from the serum. Results The average recovery of alpha-fetoprotein was 88.4% and 93.8% in the rheumatoid factor-positive and rheumatoid factor-negative serum samples, respectively. The recovery of alpha-fetoprotein was significantly lower in the rheumatoid factor-positive serum samples than in the rheumatoid factor-negative serum samples. In two of five rheumatoid factor-positive samples, a large difference was found (9.8%) between the average alpha-fetoprotein recoveries in the serially diluted and initial recoveries. Fourteen rheumatoid factor-positive serum samples were pretreated with hepatitis B surface antigen-anti-HBs complex-coated paramagnetic microparticles. The alpha-fetoprotein concentrations measured in the pretreated samples increased significantly. Conclusions It was concluded that the alpha-fetoprotein chemiluminescent microparticle immunoassay is susceptible to interference by rheumatoid factor, leading to significantly lower results. Eliminating the incidence of negative interference from rheumatoid factor should be an important goal for immunoassay providers. In the meantime

Development of a novel ultrasensitive enzyme immunoassay for human glutamic acid decarboxylase 65 antibody.

Science.gov (United States)

Numata, Satoshi; Katakami, Hideki; Inoue, Shinobu; Sawada, Hirotake; Hashida, Seiichi

2016-07-01

We developed a novel, ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for determination of glutamic acid decarboxylase autoantibody concentrations in serum samples from patients with type 2 diabetes. We developed an immune complex transfer enzyme immunoassay for glutamic acid decarboxylase autoantibody and measured glutamic acid decarboxylase autoantibody from 22 patients with type 1 diabetes, 29 patients with type 2 diabetes, and 32 healthy controls. A conventional ELISA kit identified 10 patients with type 1 diabetes and one patient with type 2 diabetes as glutamic acid decarboxylase autoantibody positive, whereas 15 patients with type 1 diabetes and six patients with type 2 diabetes were identified as glutamic acid decarboxylase autoantibody positive using immune complex transfer enzyme immunoassay. Immune complex transfer enzyme immunoassay is a highly sensitive and specific assay for glutamic acid decarboxylase autoantibody and might be clinically useful for diabetic onset prediction and early diagnosis. © The Author(s) 2016.

Molecularly Imprinted Polymer as an Antibody Substitution in Pseudo-immunoassays for Chemical Contaminants in Food and Environmental Samples.

Science.gov (United States)

Chen, Chaochao; Luo, Jiaxun; Li, Chenglong; Ma, Mingfang; Yu, Wenbo; Shen, Jianzhong; Wang, Zhanhui

2018-03-21

The chemical contaminants in food and the environment are quite harmful to food safety and human health. Rapid, accurate, and cheap detection can effectively control the potential risks derived from these chemical contaminants. Among all detection methods, the immunoassay based on the specific interaction of antibody-analyte is one of the most widely used techniques in the field. However, biological antibodies employed in the immunoassay usually cannot tolerate extreme conditions, resulting in an unstable state in both physical and chemical profiles. Molecularly imprinted polymers (MIPs) are a class of polymers with specific molecular recognition abilities, which are highly robust, showing excellent operational stability under a wide variety of conditions. Recently, MIPs have been used in biomimetic immunoassays for chemical contaminants as an antibody substitute in food and the environment. Here, we reviewed these applications of MIPs incorporated in different analytical platforms, such as enzyme-linked immunosorbent assay, fluorescent immunoassay, chemiluminescent immunoassay, electrochemical immunoassay, microfluidic paper-based immunoassay, and homogeneous immunoassay, and discussed current challenges and future trends in the use of MIPs in biomimetic immunoassays.

Heparin interferes with the radioenzymatic and homogeneous enzyme immunoassays for aminoglycosides

International Nuclear Information System (INIS)

Krogstad, D.J.; Granich, G.G.; Murray, P.R.; Pfaller, M.A.; Valdes, R.

1981-01-01

Heparin interferes with measurement of aminoglycosides in serum by biological, radioenzymatic, and homogeneous enzyme immunoassay techniques, but not with radioimmunoassay. At concentrations greater than or equal to 10 5 and greater than or equal to 3 X 10 6 USP units/L, respectively, it interferes with the radioenzymatic assay by inhibiting the gentamicin 3-acetyltransferase and kanamycin 6'-acetyltransferase enzymes used in the assay. It interferes with the homogeneous enzyme immunoassays for gentamicin and tobramycin (at concentrations greater than or equal to 10 5 and greater than or equal to10 4 USP units/L, respectively), but not with the commercially available homogeneous enzyme immunoassays for other drugs. Heparin interference with the homogeneous enzyme immunoassay for aminoglycosides requires both the heparin polyanion and glucose-6-phosphate dehydrogenase bound to a cationic aminoglycoside. This interference can be reproduced with dextran sulfate (but not dextran), and does not occur with free enzyme (glucose-6-phosphate dehydrogenase) alone. Heparin interference with these two assays and at concentrations that may be present in intravenous infusions or in seriously underfilled blood-collection tubes is described

Sensitivity-Enhancement of FRET Immunoassays by Multiple-Antibody Conjugation on Quantum Dots.

Science.gov (United States)

Annio, Giacomo; Jennings, Travis; Tagit, Oya; Hildebrandt, Niko

2018-05-23

Quantum dots (QDs) are not only advantageous for color-tuning, improved brightness, and high stability, but their nanoparticle surfaces also allow for the attachment of many biomolecules. Because IgG antibodies (ABs) are in the same size range of biocompatible QDs and the AB orientation after conjugation to the QD is often random, it is difficult to predict if few or many ABs per QD will lead to an efficient AB-QD conjugate. This is particularly true for homogeneous Förster resonance energy transfer (FRET) sandwich immunoassays, for which the ABs on the QD must bind a biomarker that needs to bind a second AB-FRET-conjugate. Here, we investigate the performance of Tb-to-QD FRET immunoassays against total prostate specific antigen (TPSA) by changing the number of ABs per QD while leaving all the other assay components unchanged. We first characterize the AB-QD conjugation by various spectroscopic, microscopic, and chromatographic techniques and then quantify the TPSA immunoassay performance regarding sensitivity, limit of detection, and dynamic range. Our results show that an increasing conjugation ratio leads to significantly enhanced FRET immunoassays. These findings will be highly important for developing QD-based immunoassays in which the concentrations of both ABs and QDs can significantly influence the assay performance.

Evaluation of the Lumipulse G TP-N Chemiluminescent Immunoassay as a Syphilis Screening Test.

Science.gov (United States)

Ortiz, Daniel A; Loeffelholz, Michael J

2017-11-01

A syphilis diagnosis is often aided by the detection of treponemal and nontreponemal antibodies. Automated treponemal antibody detection systems enable high-volume clinical laboratories to perform syphilis screening at a faster pace with lower labor costs. The Lumipulse G TP-N chemiluminescent immunoassay is an automated system that qualitatively detects IgG and IgM antibodies against Treponema pallidum antigens in human serum and plasma. To assess performance characteristics and workflow efficiency, the Lumipulse G TP-N assay was compared to the Bioplex 2200 Syphilis IgG multiplex flow immunoassay. Among the 4,134 routine and HIV samples tested by the two automated assays, the percentage of agreement was excellent at 99.0% (95% confidence interval [CI], 98.6% to 99.2%; κ, 0.89), with the Lumipulse G TP-N having a shorter time to first and subsequent results. All specimens with reactive syphilis screening results were further tested by rapid plasma reagin (RPR) and Treponema pallidum particle agglutination (TP·PA) testing ( n = 231). The results from the RPR-reactive samples ( n = 82) showed complete concordance with the two automated assays, while the TP·PA assay displayed some discrepancies. The positive percent agreement (PPA) and negative percent agreement (NPA) between the TP·PA test and the Lumipulse G TP-N test were 98.9% and 77.3%, respectively. The Bioplex 2200 Syphilis IgG immunoassay displayed a similar PPA (100%) but a substantially lower NPA (15.9%). Patient chart reviews of discrepant results suggested that the Lumipulse G TP-N assay produced 27 fewer falsely reactive results and can reduce the amount of additional confirmatory RPR and TP·PA testing needed. The analogous performance characteristics of the two automated systems indicate that the Lumipulse G TP-N assay is suitable for high-throughput syphilis screening. Copyright © 2017 American Society for Microbiology.

Uniqueness for the inverse backscattering problem for angularly controlled potentials

International Nuclear Information System (INIS)

Rakesh; Uhlmann, Gunther

2014-01-01

We consider the problem of recovering a smooth, compactly supported potential on R 3 from its backscattering data. We show that if two such potentials have the same backscattering data and the difference of the two potentials has controlled angular derivatives, then the two potentials are identical. In particular, if two potentials differ by a finite linear combination of spherical harmonics with radial coefficients and have the same backscattering data then the two potentials are identical. (paper)

Quantitative Analysis of Venus Radar Backscatter Data in ArcGIS

Science.gov (United States)

Long, S. M.; Grosfils, E. B.

2005-01-01

Ongoing mapping of the Ganiki Planitia (V14) quadrangle of Venus and definition of material units has involved an integrated but qualitative analysis of Magellan radar backscatter images and topography using standard geomorphological mapping techniques. However, such analyses do not take full advantage of the quantitative information contained within the images. Analysis of the backscatter coefficient allows a much more rigorous statistical comparison between mapped units, permitting first order selfsimilarity tests of geographically separated materials assigned identical geomorphological labels. Such analyses cannot be performed directly on pixel (DN) values from Magellan backscatter images, because the pixels are scaled to the Muhleman law for radar echoes on Venus and are not corrected for latitudinal variations in incidence angle. Therefore, DN values must be converted based on pixel latitude back to their backscatter coefficient values before accurate statistical analysis can occur. Here we present a method for performing the conversions and analysis of Magellan backscatter data using commonly available ArcGIS software and illustrate the advantages of the process for geological mapping.

Evaluation of the photon monitor backscatter in medical electron accelerators

International Nuclear Information System (INIS)

Zrenner, M.; Krieger, H.

1999-01-01

Background: Modern linear accelerators permit the use of irregular fields due to their flexible collimator systems with separately movable jaws or multileaf collimators. When using such irregular fields in the clinical practice output factors have to be corrected for enhanced backscatter to the dose monitor as compared with the conventional block shieldings. Methods: A method is presented to detect the monitor backscatter contributions to the output factor for irregular field settings. Results: The monitor backscatter factors have been measured using a telescopic device for 2 different treatment head geometries (Varian Clinac 2100C/D, General Electric Saturne 15) and for 3 photon radiation qualities (nominal energies X6, X18, X12). A method is introduced to calculate the monitor backscatter for arbitrary irregular treatment fields from the experimental data for square or rectangular fields. Conclusions: Besides the corrections for changes in phantom scatter and changes in the aperture, corrections for monitor backscatter have to be taken into account in many clinical cases. They can contribute up to more than 10% compared with the monitor values for free regular fields. (orig.) [de

A simple way to obtain backscattered electron images in a scanning transmission electron microscope.

Science.gov (United States)

Tsuruta, Hiroki; Tanaka, Shigeyasu; Tanji, Takayoshi; Morita, Chiaki

2014-08-01

We have fabricated a simple detector for backscattered electrons (BSEs) and incorporated the detector into a scanning transmission electron microscope (STEM) sample holder. Our detector was made from a 4-mm(2) Si chip. The fabrication procedure was easy, and similar to a standard transmission electron microscopy (TEM) sample thinning process based on ion milling. A TEM grid containing particle objects was fixed to the detector with a silver paste. Observations were carried out using samples of Au and latex particles at 75 and 200 kV. Such a detector provides an easy way to obtain BSE images in an STEM. © The Author 2014. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Study of multibeam techniques for bathymetry and seabottom backscatter applications

Digital Repository Service at National Institute of Oceanography (India)

Nair, R.R.; Chakraborty, B.

Indian ocean is presented using Hydrosweep-multibeam installed onboard ORV Sagarkanya. A seabottom classification model is proposed which can be applied for multibeam backscatter data. Certain aspects of the multibeam backscatter signal data processing...

HF coherent backscatter in the ionosphere: In situ measurements of SuperDARN backscatter with e-POP RRI

Science.gov (United States)

Perry, G. W.; James, H. G.; Hussey, G. C.; Howarth, A. D.; Yau, A. W.

2017-12-01

We report in situ polarimetry measurements of HF scattering obtained by the Enhanced Polar Outflow Probe (e-POP) Radio Receiver Instrument (RRI) during a coherent backscatter scattering event detected by the Saskatoon Super Dual Auroral Radar Network (SuperDARN). On April 1, 2015, e-POP conducted a 4 minute coordinated experiment with SuperDARN Saskatoon, starting at 3:38:44 UT (21:38:44 LT). Throughout the experiment, SuperDARN was transmitting at 17.5 MHz and e-POP's ground track moved in a northeastward direction, along SuperDARN's field-of-view, increasing in altitude from 331 to 352 km. RRI was tuned to 17.505 MHz, and recorded nearly 12,000 SuperDARN radar pulses during the experiment. In the first half of the experiment, radar pulses recorded by RRI were "well behaved": they retained their transmitted amplitude envelope, and their pulse-to-pulse polarization characteristics were coherent - Faraday rotation was easily measured. During the second half of the experiment the pulses showed clear signs of scattering: their amplitude envelopes became degraded and dispersed, and their pulse-to-pulse polarization characteristics became incoherent - Faraday rotation was difficult to quantify. While these pulses were being received by RRI, SuperDARN Saskatoon detected a latitudinal band of coherent backscatter at e-POP's location, indicating that the scattered pulses measured by RRI may be a signature of HF backscatter. In this presentation, we will outline the polarimetric details of the scattered pulses, and provide an analytic interpretation of RRI's measurements to give new insight into the nature of HF coherent backscatter mechanism taking place in the terrestrial ionosphere.

Interference in immunoassay

International Nuclear Information System (INIS)

Chapman, R.S.

1998-01-01

Interfering factors are evident in both limited reagent (radioimmunoassay) and excess reagent (immunometric assay) technologies and should be suspected whenever there is a discrepancy between analytical results and clinical findings in the investigation of particular diseases. The overall effect of interference in immunoassay is analytical bias in result, either positive or negative of variable magnitude. The interference maybe caused by a wide spectrum of factors from poor sample collection and handling to physiological factors e.g. lipaemia, heparin treatment, binding protein abnormalities, autoimmunity and drug treatments. The range of interfering factors is extensive and difficult to discuss effectively in a short review

A hybrid EKF and switching PSO algorithm for joint state and parameter estimation of lateral flow immunoassay models.

Science.gov (United States)

Zeng, Nianyin; Wang, Zidong; Li, Yurong; Du, Min; Liu, Xiaohui

2012-01-01

In this paper, a hybrid extended Kalman filter (EKF) and switching particle swarm optimization (SPSO) algorithm is proposed for jointly estimating both the parameters and states of the lateral flow immunoassay model through available short time-series measurement. Our proposed method generalizes the well-known EKF algorithm by imposing physical constraints on the system states. Note that the state constraints are encountered very often in practice that give rise to considerable difficulties in system analysis and design. The main purpose of this paper is to handle the dynamic modeling problem with state constraints by combining the extended Kalman filtering and constrained optimization algorithms via the maximization probability method. More specifically, a recently developed SPSO algorithm is used to cope with the constrained optimization problem by converting it into an unconstrained optimization one through adding a penalty term to the objective function. The proposed algorithm is then employed to simultaneously identify the parameters and states of a lateral flow immunoassay model. It is shown that the proposed algorithm gives much improved performance over the traditional EKF method.

Compositional analysis of silicon nitride films on Si and GaAs by backscattering spectrometry and nuclear resonance reaction analysis

International Nuclear Information System (INIS)

Kumar, Sanjiv; Raju, V.S.

2004-01-01

This paper describes the application of proton and α-backscattering spectrometry for the determination of atomic ratio of Si to N in 1100-5000 A silicon nitride films on Si and GaAs. The conventional α-Rutherford backscattering spectrometry is suitable for the analysis of films on Si; it is rather inadequate for films on GaAs due to higher background from the substrate. It is shown that these films can be analysed by 14 N(α,α) 14 N scattering with 3.5 MeV α-particles. Proton elastic scattering with enhanced cross sections for 28 Si(p,p) 28 Si and 14 N(p,p) 14 N scatterings, is also suitable for analysing films on GaAs. However, the analysis of films on Si by this technique is difficult due to interferences between the signals of Si from the film and the substrate. In addition, the hydrogen content in films is determined by 1 H( 19 F,αγ) 16 O nuclear reaction analysis using the resonance at 6.4 MeV. The combination of backscattering spectrometry with nuclear reaction analysis provides compositional analysis of ternary Si 1-(x+y) N x H y films

Nanogold–polyaniline–nanogold microspheres-functionalized molecular tags for sensitive electrochemical immunoassay of thyroid-stimulating hormone

International Nuclear Information System (INIS)

Cui Yuling; Chen Huafeng; Hou Li; Zhang Bing; Liu Bingqian; Chen Guonan; Tang Dianping

2012-01-01

Highlights: ► A novel immunosensing strategy was designed for detection of thyroid-stimulating hormone. ► Using nanogold–polyaniline–nanogold microspheres as molecular tags. ► Improvement of electrochemical activity of nanolabels. ► Combination enzyme labels with nanolabels for signal amplification. - Abstract: Methods based on nanomaterial labels have been developed for electrochemical immunosensors and immunoassays, but most involved low sensitivity. Herein a novel class of molecular tags, nanogold–polyaniline–nanogold microspheres (GPGs), was first synthesized and functionalized with horseradish peroxidase-conjugated thyroid-stimulating hormone antibody (HRP-Ab 2 ) for sensitive electrochemical immunoassay of thyroid-stimulating hormone (TSH). X-ray diffraction, confocal Raman spectroscopy, scanning electron microscope and transmission electron microscope were employed to characterize the prepared GPGs. Based on a sandwich-type immunoassay format, the assay was performed in pH 5.0 acetate buffer containing 6.0 mmol L −1 H 2 O 2 by using GPG-labeled HRP-Ab 2 as molecular tags. Compared with pure polyaniline nanospheres and gold nanoparticles alone, the GPG hybrid nanostructures increased the surface area of the nanomaterials, and enhanced the immobilized amount of HRP-Ab 2 . Several labeling protocols comprising HRP-Ab 2 , nanogold particle-labeled HRP-Ab 2 , and polyaniline nanospheres-labeled HRP-Ab 2 , were also investigated for determination of TSH and improved analytical features were obtained by using the GPG-labeled HRP-Ab 2 . With the GPG labeling method, the effects of incubation time and pH of acetate buffer on the current responses of the immunosensors were also studied. The strong attachment of HRP-Ab 2 to the GPGs resulted in a good repeatability and intermediate precision down to 7%. The dynamic concentration range spanned from 0.01 to 20 μIU mL −1 with a detection limit (LOD) of 0.005 μIU mL −1 TSH at the 3s B criterion

Effects of Hot-Spot Geometry on Backscattering and Down-Scattering Neutron Spectra

Science.gov (United States)

Mohamed, Z. L.; Mannion, O. M.; Forrest, C. J.; Knauer, J. P.; Anderson, K. S.; Radha, P. B.

2017-10-01

The measured neutron spectrum produced by a fusion experiment plays a key role in inferring observable quantities. One important observable is the areal density of an implosion, which is inferred by measuring the scattering of neutrons. This project seeks to use particle-transport simulations to model the effects of hot-spot geometry on backscattering and down-scattering neutron spectra along different lines of sight. Implosions similar to those conducted at the Laboratory of Laser Energetics are modeled by neutron transport through a DT plasma and a DT ice shell using the particle transport codes MCNP and IRIS. Effects of hot-spot geometry are obtained by ``detecting'' scattered neutrons along different lines of sight. This process is repeated for various hot-spot geometries representing known shape distortions between the hot spot and the shell. This material is based upon work supported by the Department of Energy National Nuclear Security Administration under Award Number DE-NA0001944.

Investigating the size, shape and surface roughness dependence of polarization lidars with light-scattering computations on real mineral dust particles: Application to dust particles' external mixtures and dust mass concentration retrievals

Science.gov (United States)

Mehri, Tahar; Kemppinen, Osku; David, Grégory; Lindqvist, Hannakaisa; Tyynelä, Jani; Nousiainen, Timo; Rairoux, Patrick; Miffre, Alain

2018-05-01

Our understanding of the contribution of mineral dust to the Earth's radiative budget is limited by the complexity of these particles, which present a wide range of sizes, are highly-irregularly shaped, and are present in the atmosphere in the form of particle mixtures. To address the spatial distribution of mineral dust and atmospheric dust mass concentrations, polarization lidars are nowadays frequently used, with partitioning algorithms allowing to discern the contribution of mineral dust in two or three-component particle external mixtures. In this paper, we investigate the dependence of the retrieved dust backscattering (βd) vertical profiles with the dust particle size and shape. For that, new light-scattering numerical simulations are performed on real atmospheric mineral dust particles, having determined mineralogy (CAL, DOL, AGG, SIL), derived from stereogrammetry (stereo-particles), with potential surface roughness, which are compared to the widely-used spheroidal mathematical shape model. For each dust shape model (smooth stereo-particles, rough stereo-particles, spheroids), the dust depolarization, backscattering Ångström exponent, lidar ratio are computed for two size distributions representative of mineral dust after long-range transport. As an output, two Saharan dust outbreaks involving mineral dust in two, then three-component particle mixtures are studied with Lyon (France) UV-VIS polarization lidar. If the dust size matters most, under certain circumstances, βd can vary by approximately 67% when real dust stereo-particles are used instead of spheroids, corresponding to variations in the dust backscattering coefficient as large as 2 Mm- 1·sr- 1. Moreover, the influence of surface roughness in polarization lidar retrievals is for the first time discussed. Finally, dust mass-extinction conversion factors (ηd) are evaluated for each assigned shape model and dust mass concentrations are retrieved from polarization lidar measurements. From

A Portable Immunoassay Platform for Multiplexed Detection of Biotoxins in Clinical and Environmental Samples

Energy Technology Data Exchange (ETDEWEB)

Koh, Chung-Yan [Sandia National Lab. (SNL-CA), Livermore, CA (United States); Piccini, Matthew Ernest [Sandia National Lab. (SNL-CA), Livermore, CA (United States); Cepheid, Sunnyvale, CA (United States); Schaff, Ulrich Y. [Sandia National Lab. (SNL-CA), Livermore, CA (United States); Sandstone Diagnostics, Livermore, CA (United States); Stanker, Larry H. [US Dept. of Agriculture, Albany, CA (United States). Western Regional Research Center, Foodborne Contaminants Research Unit; Cheng, Luisa W. [US Dept. of Agriculture, Albany, CA (United States). Western Regional Research Center, Foodborne Contaminants Research Unit; Ravichandran, Easwaran [Univ. of Massachusetts, Dartmouth, MA (United States); Singh, Bal-Ram [Univ. of Massachusetts, Dartmouth, MA (United States); Sommer, Greg J. [Sandia National Lab. (SNL-CA), Livermore, CA (United States); Sandstone Diagnostics, Livermore, CA (United States); Singh, Anup K. [Sandia National Lab. (SNL-CA), Livermore, CA (United States)

2015-01-01

Multiple cases of attempted bioterrorism events using biotoxins have highlighted the urgent need for tools capable of rapid screening of suspect samples in the field (e.g., mailroom and public events). We present a portable microfluidic device capable of analyzing environmental (e.g., white powder), food (e.g., milk) and clinical (e.g., blood) samples for multiplexed detection of biotoxins. The device is rapid (<15-30 min sample-to-answer), sensitive (< 0.08 pg/mL detection limit for botulinum toxin), multiplexed (up to 64 parallel assays) and capable of analyzing small volume samples (< 20 μL total sample input). The immunoassay approach (SpinDx) is based on binding of toxins in a sample to antibody-laden capture particles followed by sedimentation of particles through a density-media in a microfluidic disk and quantification using a laser-induced fluorescence detector. A direct, blinded comparison with a gold standard ELISA revealed a 5-fold more sensitive detection limit for botulinum toxin while requiring 250-fold less sample volume and a 30 minute assay time with a near unity correlation. A key advantage of the technique is its compatibility with a variety of sample matrices with no additional sample preparation required. Ultrasensitive quantification has been demonstrated from direct analysis of multiple clinical, environmental and food samples, including white powder, whole blood, saliva, salad dressing, whole milk, peanut butter, half and half, honey, and canned meat. We believe that this device can met an urgent need in screening both potentially exposed people as well as suspicious samples in mail-rooms, airports, public sporting venues and emergency rooms. The general-purpose immunodiagnostics device can also find applications in screening of infectious and systemic diseases or serve as a lab device for conducting rapid immunoassays.

Procedures for Sensitive Immunoassay

Energy Technology Data Exchange (ETDEWEB)

Givol, D. [Department of Chemical Immunology, Weizmann Institute of Science, Rehovot (Israel)

1970-02-15

Sensitive immunoassay methods should be applied to small molecules of biological importance, which are non-immunogenic by themselves, such as small peptide hormones (e.g. bradykinin), plant hormones (e.g. indoleacetic acid), nucleotides and other small molecules. Methods of binding these small molecules, as haptens, to immunogenic carriers by various cross-linking agents are described (dicyclohexylcarbodiimide, tolylene-diisocyanate and glutaraldehyde), and the considerations involved in relation to the methods of binding and the specificity of the antibodies formed are discussed. Some uses of antibody bound to bromoacetyl cellulose as an immuno adsorbent convenient for assay of immunoglobulins are described. Finally, the sensitive immunoassay method of chemically modified phage is described. This includes methods of binding small molecules (such as the dinitrophenyl group, penicillin, indoleacetic acid) or proteins (such as insulin, immunoglobulins) to phages. Methods of direct chemical conjugation, or an indirect binding via anti-phage Fab, are described. The phage inactivation method by direct plating and its modifications (such as decision technique and complex inactivation) are compared with the more simple end-point titration method. The inhibition of phage inactivation has some advantages as it does not require radioactive material, or expensive radioactive counters, and avoids the need for separation between bound and unbound antigen. Hence, if developed, it could be used as an alternative to radioimmunoassay. (author)

Relating multifrequency radar backscattering to forest biomass: Modeling and AIRSAR measurement

Science.gov (United States)

Sun, Guo-Qing; Ranson, K. Jon

1992-01-01

During the last several years, significant efforts in microwave remote sensing were devoted to relating forest parameters to radar backscattering coefficients. These and other studies showed that in most cases, the longer wavelength (i.e. P band) and cross-polarization (HV) backscattering had higher sensitivity and better correlation to forest biomass. This research examines this relationship in a northern forest area through both backscatter modeling and synthetic aperture radar (SAR) data analysis. The field measurements were used to estimate stand biomass from forest weight tables. The backscatter model described by Sun et al. was modified to simulate the backscattering coefficients with respect to stand biomass. The average number of trees per square meter or radar resolution cell, and the average tree height or diameter breast height (dbh) in the forest stand are the driving parameters of the model. The rest of the soil surface, orientation, and size distributions of leaves and branches, remain unchanged in the simulations.

Acoustic backscatter at a Red Sea whale shark aggregation site

KAUST Repository

Hozumi, Aya; Kaartvedt, Stein; Rø stad, Anders; Berumen, Michael L.; Cochran, Jesse E.M.; Jones, Burton

2018-01-01

An aggregation of sexually immature whale sharks occurs at a coastal submerged reef near the Saudi Arabian Red Sea coast each spring. We tested the hypothesis that these megaplanktivores become attracted to a prey biomass peak coinciding with their aggregation. Acoustic backscatter of the water column at 120 kHz and 333 kHz –a proxy for potential prey biomass –was continuously measured spanning the period prior to, during, and subsequent to the seasonal whale shark aggregations. No peak in acoustic backscatter was observed at the time of the aggregation. However, we observed a decrease in acoustic backscatter in the last days of deployment, which coincided the trailing end of whale shark season. Organisms forming the main scattering layer performed inverse diel vertical migration, with backscatter peaking at mid-depths during the day and in the deeper half of the water column at night. Target strength analyses suggested the backscatter was likely composed of fish larvae. Subsurface foraging behavior of the whale sharks within this aggregation has not been described, yet this study does not support the hypothesis that seasonal peaks in local whale shark abundance correspond to similar peaks in prey availability.

Acoustic backscatter at a Red Sea whale shark aggregation site

KAUST Repository

Hozumi, Aya

2018-03-28

An aggregation of sexually immature whale sharks occurs at a coastal submerged reef near the Saudi Arabian Red Sea coast each spring. We tested the hypothesis that these megaplanktivores become attracted to a prey biomass peak coinciding with their aggregation. Acoustic backscatter of the water column at 120 kHz and 333 kHz –a proxy for potential prey biomass –was continuously measured spanning the period prior to, during, and subsequent to the seasonal whale shark aggregations. No peak in acoustic backscatter was observed at the time of the aggregation. However, we observed a decrease in acoustic backscatter in the last days of deployment, which coincided the trailing end of whale shark season. Organisms forming the main scattering layer performed inverse diel vertical migration, with backscatter peaking at mid-depths during the day and in the deeper half of the water column at night. Target strength analyses suggested the backscatter was likely composed of fish larvae. Subsurface foraging behavior of the whale sharks within this aggregation has not been described, yet this study does not support the hypothesis that seasonal peaks in local whale shark abundance correspond to similar peaks in prey availability.

Optimal Time Allocation in Backscatter Assisted Wireless Powered Communication Networks

Science.gov (United States)

Lyu, Bin; Yang, Zhen; Gui, Guan; Sari, Hikmet

2017-01-01

This paper proposes a wireless powered communication network (WPCN) assisted by backscatter communication (BackCom). This model consists of a power station, an information receiver and multiple users that can work in either BackCom mode or harvest-then-transmit (HTT) mode. The time block is mainly divided into two parts corresponding to the data backscattering and transmission periods, respectively. The users first backscatter data to the information receiver in time division multiple access (TDMA) during the data backscattering period. When one user works in the BackCom mode, the other users harvest energy from the power station. During the data transmission period, two schemes, i.e., non-orthogonal multiple access (NOMA) and TDMA, are considered. To maximize the system throughput, the optimal time allocation policies are obtained. Simulation results demonstrate the superiority of the proposed model. PMID:28587171

Self ordering threshold and superradiant backscattering to slow a fast gas beam in a ring cavity with counter propagating pump.

Science.gov (United States)

Maes, C; Asbóth, J K; Ritsch, H

2007-05-14

We study the dynamics of a fast gaseous beam in a high Q ring cavity counter propagating a strong pump laser with large detuning from any particle optical resonance. As spontaneous emission is strongly suppressed the particles can be treated as polarizable point masses forming a dynamic moving mirror. Above a threshold intensity the particles exhibit spatial periodic ordering enhancing collective coherent backscattering which decelerates the beam. Based on a linear stability analysis in their accelerated rest frame we derive analytic bounds for the intensity threshold of this selforganization as a function of particle number, average velocity, kinetic temperature, pump detuning and resonator linewidth. The analytical results agree well with time dependent simulations of the N-particle motion including field damping and spontaneous emission noise. Our results give conditions which may be easily evaluated for stopping and cooling a fast molecular beam.

Interference phenomena at backscattering by ice crystals of cirrus clouds.

Science.gov (United States)

Borovoi, Anatoli; Kustova, Natalia; Konoshonkin, Alexander

2015-09-21

It is shown that light backscattering by hexagonal ice crystals of cirrus clouds is formed within the physical-optics approximation by both diffraction and interference phenomena. Diffraction determines the angular width of the backscattering peak and interference produces the interference rings inside the peak. By use of a simple model for distortion of the pristine hexagonal shape, we show that the shape distortion leads to both oscillations of the scattering (Mueller) matrix within the backscattering peak and to a strong increase of the depolarization, color, and lidar ratios needed for interpretation of lidar signals.

Abbott prism: a multichannel heterogeneous chemiluminescence immunoassay analyzer.

Science.gov (United States)

Khalil, O S; Zurek, T F; Tryba, J; Hanna, C F; Hollar, R; Pepe, C; Genger, K; Brentz, C; Murphy, B; Abunimeh, N

1991-09-01

We describe a multichannel heterogeneous immunoassay analyzer in which a sample is split between disposable reaction trays in a group of linear tracks. The system's pipettor uses noninvasive sensing of the sample volume and disposable pipet tips. Each assay track has (a) a conveyor belt for moving reaction trays to predetermined functional stations, (b) temperature-controlled tunnels, (c) noncontact transfer of the reaction mixture between incubation and detection wells, and (d) single-photon counting to detect a chemiluminescence (CL) signal from the captured immunochemical product. A novel disposable reaction tray, with separate reaction and detection wells and self-contained fluid removal, is used in conjunction with the transfer device on the track to produce a carryover-free system. The linear immunoassay track has nine predetermined positions for performing individual assay steps. Assay step sequence and timing is selected by changing the location of the assay modules between these predetermined positions. The assay methodology, a combination of microparticle capture and direct detection of a CL signal on a porous matrix, offers excellent sensitivity, specificity, and ease of automation. Immunoassay configurations have been tested for hepatitis B surface antigen and for antibodies to hepatitis B core antigen, hepatitis C virus, human immunodeficiency virus I and II, and human T-cell leukemia virus I and II.

Backscatter and attenuation characterization of ventricular myocardium

Science.gov (United States)

Gibson, Allyson Ann

2009-12-01

This Dissertation presents quantitative ultrasonic measurements of the myocardium in fetal hearts and adult human hearts with the goal of studying the physics of sound waves incident upon anisotropic and inhomogeneous materials. Ultrasound has been used as a clinical tool to assess heart structure and function for several decades. The clinical usefulness of this noninvasive approach has grown with our understanding of the physical mechanisms underlying the interaction of ultrasonic waves with the myocardium. In this Dissertation, integrated backscatter and attenuation analyses were performed on midgestational fetal hearts to assess potential differences in the left and right ventricular myocardium. The hearts were interrogated using a 50 MHz transducer that enabled finer spatial resolution than could be achieved at more typical clinical frequencies. Ultrasonic data analyses demonstrated different patterns and relative levels of backscatter and attenuation from the myocardium of the left ventricle and the right ventricle. Ultrasonic data of adult human hearts were acquired with a clinical imaging system and quantified by their magnitude and time delay of cyclic variation of myocardial backscatter. The results were analyzing using Bayes Classification and ROC analysis to quantify potential advantages of using a combination of two features of cyclic variation of myocardial backscatter over using only one or the other feature to distinguish between groups of subjects. When the subjects were classified based on hemoglobin A1c, the homeostasis model assessment of insulin resistance, and the ratio of triglyceride to high-density lipoprotein-cholesterol, differences in the magnitude and normalized time delay of cyclic variation of myocardial backscatter were observed. The cyclic variation results also suggested a trend toward a larger area under the ROC curve when information from magnitude and time delay of cyclic variation is combined using Bayes classification than when

Standards for backscattering analysis

International Nuclear Information System (INIS)

Mitchell, I.V.; Eschbach, H.L.

1978-01-01

The need for backscattering standards appears to be twofold and depends on the uses and requirements of the users. The first is as a calibrated reference by which samples of a similar nature to the standard may be absolutely compared. The second is as a means of intercomparing the relative results obtained by different laboratories using, as near as possible, identical samples. This type of comparison is of a relative nature and the absolute values are not necessarily required. In the present work the authors try to satisfy both needs by providing identical samples which have been absolutely calibrated to a high accuracy. Very thin copper and vanadium layers were evaporated onto bismuth implanted silicon crystals and on glass plates under carefully controlled conditions. The mass of the deposits was determined in situ using a sensitive UHV microbalance. In addition, two quartz oscillator monitors were used. The samples have been analysed by Rutherford backscattering and the absolute quantity of bismuth determined by a comparison with the known amounts of deposited material. (Auth.)

Immunoassay for determination of trilobolide

Czech Academy of Sciences Publication Activity Database

Huml, L.; Jurášek, M.; Mikšátková, P.; Zimmermann, T.; Tomanová, P.; Buděšínský, Miloš; Rottnerová, Z.; Šimková, M.; Harmatha, Juraj; Kmoníčková, Eva; Lapčík, O.; Drašar, P. B.

2017-01-01

Roč. 117, Jan (2017), s. 105-111 ISSN 0039-128X. [Conference on Isoprenoids /23./. Minsk, 04.09.2016-07.09.2016] Institutional support: RVO:61388963 ; RVO:68378041 Keywords : trilobolide * avidin-biotin * ELISA * Laser trilobum * synthesis * immunoassay Subject RIV: CE - Biochemistry; FR - Pharmacology ; Medidal Chemistry (UEM-P) OBOR OECD: Biochemical research methods; Pharmacology and pharmacy (UEM-P) Impact factor: 2.282, year: 2016

Development of immunoassays for detecting clothianidin residue in agricultural products.

Science.gov (United States)

Li, Ming; Sheng, Enze; Cong, Lujing; Wang, Minghua

2013-04-17

Two enzyme-linked immunosorbent assays (ELISAs) based on polyclonal antibodies (PcAbs) for clothianidin are described: colorimetric detection format (ELISA) and pattern of chemiluminescent assay (CLEIA). Clothianidin hapten was synthesized and conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to produce immunogen and coating antigen. Anticlothianidin PcAbs were obtained from immunized New Zealand white rabbits. Under optimal conditions, the half-maximal inhibition concentration (IC₅₀) and the limit of detection (LOD, IC₂₀) of clothianidin were 0.046 and 0.0028 mg/L for the ELISA and 0.015 and 0.0014 mg/L for the CLEIA, respectively. There were no obvious cross-reactivities of the antibodies with its analogues except for dinotefuran. Recoveries of 76.4-116.4% for the immunoassays were achieved from spiked samples. The results of immunoassays for the spiked and authentic samples were largely consistent with gas chromatography. Therefore, the proposed immunoassays would be convenient and satisfactory analytical methods for the monitoring of clothianidin in agricultural products.

Aerosol backscatter measurements at 10.6 microns with airborne and ground-based CO2 Doppler lidars over the Colorado High Plains. II - Backscatter structure

Science.gov (United States)

Bowdle, David A.; Rothermel, Jeffry; Vaughan, J. Michael; Post, Madison J.

1991-01-01

Measurements of tropospheric aerosol volume backscatter coefficients at 10.6-microns wavelength were obtained with airborne continuous wave and ground-based pulsed CO2 Doppler lidars over the Colorado High Plains during a 20-day period in summer 1982. A persistent 'background' layer was found between 6- and 10-km altitude, with a generally uniform backscatter mixing ratio of about 10 to the -10th sq m/kg per sr. The upper boundary of this background layer varied with the tropopause height; the lower boundary varied with the strength and diurnal cycle of convective mixing in the planetary boundary layer (PBL). For quiescent meteorological conditions, the transition from the PBL to the background layer was usually very sharp, with backscatter decreases sometimes as large as 3 decades in about 70 m. Sharp gradients were also found at the boundaries of shallow (tens of meters) subvisible cirrus clouds. For less stable conditions, associated with vertical aerosol transport by deep cumuliform clouds, backscatter tended to decrease exponentially with altitude.

Passive amplification of a fiber laser in a Fabry-Perot cavity: application to gamma-ray production by Compton backscattering

International Nuclear Information System (INIS)

Labaye, F.

2012-01-01

One of the critical points of the International Linear Collider (ILC) is the polarized positrons source. Without going through further explanation on the physical process of polarized positrons production, we point out that they are produced when circularly polarized gamma rays interact with mater. Thus, the critical point is the circularly polarized gamma-ray source. A technical solution for this source is the Compton backscattering and in the end, this thesis takes place in the framework for the design of a high average power laser systems enslaved to Fabry-Perot cavities for polarized gamma-ray production by Compton backscattering. In the first part, we present this thesis context, the Compton backscattering principle and the choice for an optical architecture based on a fiber laser and a Fabry-Perot cavity. We finish by enumerating several possible applications for Compton backscattering which shows that the work presented here might benefits from technology transfer through others research fields. In the second part, we present the different fiber laser architecture studied as well as the results obtained. In the third part, we remind the operating principle of a Fabry-Perot cavity and present the one used for our experiment as well as its specificities. In the fourth part, we address the Compton backscattering experiment which enables us to present the joint utilization of a fiber laser and a Fabry-Perot cavity in a particles accelerator to generate gamma rays for the first time to our knowledge. This experiment took place in the Accelerator Test Facility (ATF). The experimental apparatus as well as the results obtained are thus presented. In the end, we summarize the results presented in this manuscript and propose different evolution possibilities for the system in a general conclusion. (author)

Backscatter Correction Algorithm for TBI Treatment Conditions

Energy Technology Data Exchange (ETDEWEB)

Sanchez-Nieto, B.; Sanchez-Doblado, F.; Arrans, R.; Terron, J.A. [Dpto. Fisiología Médica y Biofísica, Universidad de Sevilla, Avda. Sánchez Pizjuán, 4. E-41009, Sevilla (Spain); Errazquin, L. [Servicio Oncología Radioterápica, Hospital Univ.V. Macarena. Dr. Fedriani, s/n. E-41009, Sevilla (Spain)

2015-01-15

The accuracy requirements in target dose delivery is, according to ICRU, ±5%. This is so not only in standard radiotherapy but also in total body irradiation (TBI). Physical dosimetry plays an important role in achieving this recommended level. The semi-infinite phantoms, customarily used for dosimetry purposes, give scatter conditions different to those of the finite thickness of the patient. So dose calculated in patient’s points close to beam exit surface may be overestimated. It is then necessary to quantify the backscatter factor in order to decrease the uncertainty in this dose calculation. The backward scatter has been well studied at standard distances. The present work intends to evaluate the backscatter phenomenon under our particular TBI treatment conditions. As a consequence of this study, a semi-empirical expression has been derived to calculate (within 0.3% uncertainty) the backscatter factor. This factor depends lineally on the depth and exponentially on the underlying tissue. Differences found in the qualitative behavior with respect to standard distances are due to scatter in the bunker wall close to the measurement point.

AN ENVIRONMENTAL TECHNOLOGY VERIFICATION (ETV) TESTING OF THREE IMMUNOASSAY TEST KITS FOR ANTHRAX, BOTULINUM TOXIN AND RICIN

Science.gov (United States)

Immunoassay test kits are based on immunoassay methods, where specific antibodies are used to detect and measure the contaminants of interest. Immunoassay test kits rely on the reaction of a contaminant or antigen with a selective antibody to give a product that can be measures....

Simplified immunoassay for rapid Dengue serotype diagnosis, revealing insensitivity to non-specific binding interference

Directory of Open Access Journals (Sweden)

Fernanda C.C.L. Loureiro

2017-04-01

Full Text Available Proof of concept of an immunoassay, which is easy to implement, for rapid Dengue virus (DENV serotype diagnosis, in the early infection stage, is reported. The four-layer assay is immobilized onto a thin gold film and relies on a low cost, disposable polymer biochip for optical surface plasmon resonance sensing and detection. The protocol comprises Neutravidin-Biotin mediated monoclonal antibody (MAB attachment as the functionalized sensing element. Formation of the MAB-DENV complex results in a pronounced thickness change that is optically recorded in real time, employing a microfluidic set-up. Virus presence is confirmed by atomic force microscopy from the same sample. Serum samples were collected from a patient in acute febrile state. Simultaneous serological analysis by means of the reverse transcription polymerase chain reaction, independently, confirmed presence of DENV2 and DENV3. The protocol proved applicable in presence of strong non-specific binding interference that originates from, and is caused by, various blood, serum and other body fluid constituents. False positive indications for both, negative serum and blood control samples were not observed. The achievable limit of detection was estimated to be 2×104 particles/ml. Eventually, the method can be modified towards detection of other viruses by using the same protocol. Keywords: Immuno-assay, Dengue virus detection, Non-specific binding

History of inductively coupled plasma mass spectrometry-based immunoassays

International Nuclear Information System (INIS)

Giesen, Charlotte; Waentig, Larissa; Panne, Ulrich; Jakubowski, Norbert

2012-01-01

The analysis of biomolecules requires highly sensitive and selective detection methods capable of tolerating a complex, biological matrix. First applications of biomolecule detection by ICP-MS relied on the use of heteroelements as a label for quantification. However, the combination of immunoassays and ICP-MS facilitates multiparametric analyses through elemental tagging, and provides a powerful alternative to common bioanalytical methods. This approach extends the detection of biomarkers in clinical diagnosis, and has the potential to provide a deeper understanding of the investigated biological system. The results might lead to the detection of diseases at an early stage, or guide treatment plans. Immunoassays are well accepted and established for diagnostic purposes, albeit ICP-MS is scarcely applied for the detection of immune-based assays. However, the screening of biomarkers demands high throughput and multiplex/multiparametric techniques, considering the variety of analytes to be queried. Finally, quantitative information on the expression level of biomarkers is highly desirable to identify abnormalities in a given organism. Thus, it is the aim of this review to introduce the fundamentals, and to discuss the enormous strength of ICP-MS for the detection of different immunoassays on the basis of selected applications, with a special focus on LA‐ICP‐MS. - Highlights: ► We discuss the fundamentals of elemental tagging for ICP‐MS applications. ► We propose a definition for the expressions “label” and “tag”. ► We highlight LA‐ICP‐MS‐based heteroelement detection. ► We give an historic overview on ICP-MS and LA‐ICP‐MS-based immunoassays. ► In a personal outlook, we discuss future improvements realistically attainable.

BIOTIN INTERFERENCE WITH ROUTINE CLINICAL IMMUNOASSAYS: UNDERSTAND THE CAUSES AND MITIGATE THE RISKS.

Science.gov (United States)

Samarasinghe, Shanika; Meah, Farah; Singh, Vinita; Basit, Arshi; Emanuele, Nicholas; Emanuele, Mary Ann; Mazhari, Alaleh; Holmes, Earle W

2017-08-01

The objectives of this report are to review the mechanisms of biotin interference with streptavidin/biotin-based immunoassays, identify automated immunoassay systems vulnerable to biotin interference, describe how to estimate and minimize the risk of biotin interference in vulnerable assays, and review the literature pertaining to biotin interference in endocrine function tests. The data in the manufacturer's "Instructions for Use" for each of the methods utilized by seven immunoassay system were evaluated. We also conducted a systematic search of PubMed/MEDLINE for articles containing terms associated with biotin interference. Available original reports and case series were reviewed. Abstracts from recent scientific meetings were also identified and reviewed. The recent, marked, increase in the use of over-the-counter, high-dose biotin supplements has been accompanied by a steady increase in the number of reports of analytical interference by exogenous biotin in the immunoassays used to evaluate endocrine function. Since immunoassay methods of similar design are also used for the diagnosis and management of anemia, malignancies, autoimmune and infectious diseases, cardiac damage, etc., biotin-related analytical interference is a problem that touches every area of internal medicine. It is important for healthcare personnel to become more aware of immunoassay methods that are vulnerable to biotin interference and to consider biotin supplements as potential sources of falsely increased or decreased test results, especially in cases where a lab result does not correlate with the clinical scenario. FDA = U.S. Food & Drug Administration FT3 = free tri-iodothyronine FT4 = free thyroxine IFUs = instructions for use LH = luteinizing hormone PTH = parathyroid hormone SA/B = streptavidin/biotin TFT = thyroid function test TSH = thyroid-stimulating hormone.

Analytical purpose electron backscattering system

International Nuclear Information System (INIS)

Desdin, L.; Padron, I.; Laria, J.

1996-01-01

In this work an analytical purposes electron backscattering system improved at the Center of Applied Studies for Nuclear Development is described. This system can be applied for fast, exact and nondestructive testing of binary and AL/Cu, AL/Ni in alloys and for other applications

Power Control for Passive QAM Multisensor Backscatter Communication Systems

Directory of Open Access Journals (Sweden)

Shengbo Hu

2017-01-01

Full Text Available To achieve good quality of service level such as throughput, power control is of great importance to passive quadrature amplitude modulation (QAM multisensor backscatter communication systems. First, we established the RF energy harvesting model and gave the energy condition. In order to minimize the interference of subcarriers and increase the spectral efficiency, then, the colocated passive QAM backscatter communication signal model is presented and the nonlinear optimization problems of power control are solved for passive QAM backscatter communication systems. Solutions include maximum and minimum access interval, the maximum and minimum duty cycle, and the minimal RF-harvested energy under the energy condition for node operating. Using the solutions above, the maximum throughput of passive QAM backscatter communication systems is analyzed and numerical calculation is made finally. Numerical calculation shows that the maximal throughput decreases with the consumed power and the number of sensors, and the maximum throughput is decreased quickly with the increase of the number of sensors. Especially, for a given consumed power of sensor, it can be seen that the throughput decreases with the duty cycle and the number of sensors has little effect on the throughput.

On particles in the Arctic stratosphere

Directory of Open Access Journals (Sweden)

T. S. Jørgensen

2003-06-01

Full Text Available Soon after the discovery of the Antarctic ozone hole it became clear that particles in the polar stratosphere had an infl uence on the destruction of the ozone layer. Two major types of particles, sulphate aerosols and Polar Stratospheric Clouds (PSCs, provide the surfaces where fast heterogeneous chemical reactions convert inactive halogen reservoir species into potentially ozone-destroying radicals. Lidar measurements have been used to classify the PSCs. Following the Mt. Pinatubo eruption in June 1991 it was found that the Arctic stratosphere was loaded with aerosols, and that aerosols observed with lidar and ozone observed with ozone sondes displayed a layered structure, and that the aerosol and ozone contents in the layers frequently appeared to be negatively correlated. The layered structure was probably due to modulation induced by the dynamics at the edge of the polar vortex. Lidar observations of the Mt. Pinatubo aerosols were in several cases accompanied by balloon-borne backscatter soundings, whereby backscatter measurements in three different wavelengths made it possible to obtain information about the particle sizes. An investigation of the infl uence of synoptic temperature histories on the physical properties of PSC particles has shown that most of the liquid type 1b particles were observed in the process of an ongoing, relatively fast, and continuous cooling from temperatures clearly above the nitric acid trihydrate condensation temperature (TNAT. On the other hand, it appeared that a relatively long period, with a duration of at least 1-2 days, at temperatures below TNAT provide the conditions which may lead to the production of solid type 1a PSCs.

Snowfall retrieval at X, Ka and W bands: consistency of backscattering and microphysical properties using BAECC ground-based measurements

Directory of Open Access Journals (Sweden)

M. T. Falconi

2018-05-01

Full Text Available Radar-based snowfall intensity retrieval is investigated at centimeter and millimeter wavelengths using co-located ground-based multi-frequency radar and video-disdrometer observations. Using data from four snowfall events, recorded during the Biogenic Aerosols Effects on Clouds and Climate (BAECC campaign in Finland, measurements of liquid-water-equivalent snowfall rate S are correlated to radar equivalent reflectivity factors Ze, measured by the Atmospheric Radiation Measurement (ARM cloud radars operating at X, Ka and W frequency bands. From these combined observations, power-law Ze–S relationships are derived for all three frequencies considering the influence of riming. Using microwave radiometer observations of liquid water path, the measured precipitation is divided into lightly, moderately and heavily rimed snow. Interestingly lightly rimed snow events show a spectrally distinct signature of Ze–S with respect to moderately or heavily rimed snow cases. In order to understand the connection between snowflake microphysical and multi-frequency backscattering properties, numerical simulations are performed by using the particle size distribution provided by the in situ video disdrometer and retrieved ice particle masses. The latter are carried out by using both the T-matrix method (TMM applied to soft-spheroid particle models with different aspect ratios and exploiting a pre-computed discrete dipole approximation (DDA database for rimed aggregates. Based on the presented results, it is concluded that the soft-spheroid approximation can be adopted to explain the observed multi-frequency Ze–S relations if a proper spheroid aspect ratio is selected. The latter may depend on the degree of riming in snowfall. A further analysis of the backscattering simulations reveals that TMM cross sections are higher than the DDA ones for small ice particles, but lower for larger particles. The differences of computed cross sections for larger and

Snowfall retrieval at X, Ka and W bands: consistency of backscattering and microphysical properties using BAECC ground-based measurements

Science.gov (United States)

Tecla Falconi, Marta; von Lerber, Annakaisa; Ori, Davide; Silvio Marzano, Frank; Moisseev, Dmitri

2018-05-01

Radar-based snowfall intensity retrieval is investigated at centimeter and millimeter wavelengths using co-located ground-based multi-frequency radar and video-disdrometer observations. Using data from four snowfall events, recorded during the Biogenic Aerosols Effects on Clouds and Climate (BAECC) campaign in Finland, measurements of liquid-water-equivalent snowfall rate S are correlated to radar equivalent reflectivity factors Ze, measured by the Atmospheric Radiation Measurement (ARM) cloud radars operating at X, Ka and W frequency bands. From these combined observations, power-law Ze-S relationships are derived for all three frequencies considering the influence of riming. Using microwave radiometer observations of liquid water path, the measured precipitation is divided into lightly, moderately and heavily rimed snow. Interestingly lightly rimed snow events show a spectrally distinct signature of Ze-S with respect to moderately or heavily rimed snow cases. In order to understand the connection between snowflake microphysical and multi-frequency backscattering properties, numerical simulations are performed by using the particle size distribution provided by the in situ video disdrometer and retrieved ice particle masses. The latter are carried out by using both the T-matrix method (TMM) applied to soft-spheroid particle models with different aspect ratios and exploiting a pre-computed discrete dipole approximation (DDA) database for rimed aggregates. Based on the presented results, it is concluded that the soft-spheroid approximation can be adopted to explain the observed multi-frequency Ze-S relations if a proper spheroid aspect ratio is selected. The latter may depend on the degree of riming in snowfall. A further analysis of the backscattering simulations reveals that TMM cross sections are higher than the DDA ones for small ice particles, but lower for larger particles. The differences of computed cross sections for larger and smaller particles are

Nanoparticle-based immunosensors and immunoassays for aflatoxins

Energy Technology Data Exchange (ETDEWEB)

Wang, Xu; Niessner, Reinhard [Institute of Hydrochemistry and Chair of Analytical Chemistry, Technische Universität München, Marchioninistrasse 17, D-81377 München (Germany); Tang, Dianping [Key Laboratory of Analysis and Detection for Food Safety, MOE & Fujian Province, Department of Chemistry, Fuzhou University, Fuzhou 350108 (China); Knopp, Dietmar, E-mail: dietmar.knopp@ch.tum.de [Institute of Hydrochemistry and Chair of Analytical Chemistry, Technische Universität München, Marchioninistrasse 17, D-81377 München (Germany)

2016-03-17

Aflatoxins are naturally existing mycotoxins produced mainly by Aspergillus flavus and Aspergillus parasiticus, present in a wide range of food and feed products. Because of their extremely high toxicity and carcinogenicity, strict control of maximum residue levels of aflatoxins in foodstuff is set by many countries. In daily routine, different chromatographic methods are used almost exclusively. As supplement, in several companies enzyme immunoassay-based sample testing as primary screening is performed. Recently, nanomaterials such as noble metal nanoparticles, magnetic particles, carbon nanomaterials, quantum dots, and silica nanomaterials are increasingly utilized for aflatoxin determination to improve the sensitivity and simplify the detection. They are employed either as supports for the immobilization of biomolecules or as electroactive or optical labels for signal transduction and amplification. Several nanoparticle-based electrochemical, piezoelectric, optical, and immunodipstick assays for aflatoxins have been developed. In this review, we summarize these recent advances and illustrate novel concepts and promising applications in the field of food safety. - Highlights: • Novel concepts and promising applications of nanoparticle-based immunological methods for the determination of aflatoxins. • Inclusion of most important nanomaterials and hybrid nanostructures. • Inclusion of electrochemical, optical and mass-sensitive biosensors as well as optical and immunochromatographic assays.

Mitigating the effect of optical back-scatter in multispectral underwater imaging

International Nuclear Information System (INIS)

Mortazavi, Halleh; Oakley, John P; Barkat, Braham

2013-01-01

Multispectral imaging is a very useful technique for extracting information from the underwater world. However, optical back-scatter changes the intensity value in each spectral band and this distorts the estimated spectrum. In this work, a filter is used to detect the level of optical back-scatter in each spectral band from a set of multispectral images. Extraction of underwater object spectra can be done by subtracting the estimated level of optical back-scatter and scaling the remainder in each spectral band from the captured image in the corresponding band. An experiment has been designed to show the performance of the proposed filter for correcting the set of multispectral underwater images and recovering the pixel spectra. The multispectral images are captured by a B/W CCD digital camera with a fast tunable liquid-crystal filter in 33 narrow spectral bands in clear and different levels of turbid water. Reference estimates for the optical back-scatter spectra are found by comparing a clear and a degraded set of multispectral images. The accuracy and consistency of the proposed method, the extended Oakley–Bu cost function, is examined by comparing the estimated values with the reference level of an optical back-scatter spectrum. The same comparison is made for the simple estimation approach. The results show that the simple method is not reliable and fail to estimate the level of optical back-scatter spectrum accurately. The results from processing experimental images in turbid water show that the effect of optical back-scatter can be mitigated in the image of each spectral band and, as a result, the spectra of the object can be recovered. However, for a very high level of turbid water the recovery is limited because of the effect of extinction. (paper)

Dependence of equilibrium properties of channeled particles on transverse quasi temperature

International Nuclear Information System (INIS)

Kashlev, Yu.A.

2006-01-01

Quasi-equilibrium and kinetic characteristics of channeled particles are investigated by methods of nonequilibrium statistical thermodynamics. The equilibrium equation of the transverse energy of fast particles and the equilibrium equation of the transverse momentum of particles are derived. It is shown that equilibrium equations solution permits to obtain the expression for the transverse quasi-temperature of the channeled particle subsystem. The quasi-equilibrium angular distribution of particles after transmission through a thin monocrystal and the angular distribution at backscattering are studied. The evaluated data of the transverse quasi-temperature are presented for the case of iodine ion channeling through silver crystals [ru

Automation on an Open-Access Platform of Alzheimer's Disease Biomarker Immunoassays.

Science.gov (United States)

Gille, Benjamin; Dedeene, Lieselot; Stoops, Erik; Demeyer, Leentje; Francois, Cindy; Lefever, Stefanie; De Schaepdryver, Maxim; Brix, Britta; Vandenberghe, Rik; Tournoy, Jos; Vanderstichele, Hugo; Poesen, Koen

2018-04-01

The lack of (inter-)laboratory standardization has hampered the application of universal cutoff values for Alzheimer's disease (AD) cerebrospinal fluid (CSF) biomarkers and their transfer to general clinical practice. The automation of the AD biomarker immunoassays is suggested to generate more robust results than using manual testing. Open-access platforms will facilitate the integration of automation for novel biomarkers, allowing the introduction of the protein profiling concept. A feasibility study was performed on an automated open-access platform of the commercial immunoassays for the 42-amino-acid isoform of amyloid-β (Aβ 1-42 ), Aβ 1-40 , and total tau in CSF. Automated Aβ 1-42 , Aβ 1-40 , and tau immunoassays were performed within predefined acceptance criteria for bias and imprecision. Similar accuracy was obtained for ready-to-use calibrators as for reconstituted lyophilized kit calibrators. When compared with the addition of a standard curve in each test run, the use of a master calibrator curve, determined before and applied to each batch analysis as the standard curve, yielded an acceptable overall bias of -2.6% and -0.9% for Aβ 1-42 and Aβ 1-40 , respectively, with an imprecision profile of 6.2% and 8.4%, respectively. Our findings show that transfer of commercial manual immunoassays to fully automated open-access platforms is feasible, as it performs according to universal acceptance criteria.

Super-virtual Interferometric Separation and Enhancement of Back-scattered Surface Waves

KAUST Repository

Guo, Bowen; Hanafy, Sherif; Schuster, Gerard T.

2015-01-01

Back-scattered surface waves can be migrated to detect near-surface reflectors with steep dips. A robust surface-wave migration requires the prior separation of the back-scattered surface-wave events from the data. This separation is often difficult

Retrieving forest stand parameters from SAR backscatter data using a neural network trained by a canopy backscatter model

International Nuclear Information System (INIS)

Wang, Y.; Dong, D.

1997-01-01

It was possible to retrieve the stand mean dbh (tree trunk diameter at breast height) and stand density from the Jet Propulsion Laboratory (JPL) Airborne Synthetic Aperture Radar (AIRSAR) backscatter data by using threelayered perceptron neural networks (NNs). Two sets of NNs were trained by the Santa Barbara microwave canopy backscatter model. One set of the trained NNs was used to retrieve the stand mean dbh, and the other to retrieve the stand density. Each set of the NNs consisted of seven individual NNs for all possible combinations of one, two, and three radar wavelengths. Ground and multiple wavelength AIRSAR backscatter data from two ponderosa pine forest stands near Mt. Shasta, California (U.S.A.) were used to evaluate the accuracy of the retrievals. The r.m.s. and relative errors of the retrieval for stand mean dbh were 6.1 cm and 15.6 per cent for one stand (St2), and 3.1 cm and 6.7 per cent for the other stand (St11). The r.m.s. and relative errors of the retrieval for stand density were 71.2 treesha-1 and 23.0 per cent for St2, and 49.7 treesha-1 and 21.3 per cent for St11. (author)

Design and Fabrication of a PDMS Microchip Based Immunoassay

Energy Technology Data Exchange (ETDEWEB)

Shao, Guocheng; Wang, Wanjun; Wang, Jun; Lin, Yuehe

2010-07-01

In this paper, we describe the design and fabrication process of a polydimethylsiloxane (PDMS) microchip for on-chip multiplex immunoassay application. The microchip consists of a PDMS microfluidic channel layer and a micro pneumatic valve control layer. By selectively pressurizing the pneumatic microvalves, immuno reagents were controlled to flow and react in certain fluidic channel sites. Cross contamination was prevented by tightly closed valves. Our design was proposed to utilize PDMS micro channel surface as the solid phase immunoassay substrate and simultaneously detect four targets antigens on chip. Experiment result shows that 20psi valve pressure is sufficient to tightly close a 200µm wide micro channel with flow rate up to 20µl/min.

Compositional Signatures in Acoustic Backscatter Over Vegetated and Unvegetated Mixed Sand-Gravel Riverbeds

Science.gov (United States)

Buscombe, D.; Grams, P. E.; Kaplinski, M. A.

2017-10-01

Multibeam acoustic backscatter has considerable utility for remote characterization of spatially heterogeneous bed sediment composition over vegetated and unvegetated riverbeds of mixed sand and gravel. However, the use of high-frequency, decimeter-resolution acoustic backscatter for sediment classification in shallow water is hampered by significant topographic contamination of the signal. In mixed sand-gravel riverbeds, changes in the abiotic composition of sediment (such as homogeneous sand to homogeneous gravel) tend to occur over larger spatial scales than is characteristic of small-scale bedform topography (ripples, dunes, and bars) or biota (such as vascular plants and periphyton). A two-stage method is proposed to filter out the morphological contributions to acoustic backscatter. First, the residual supragrain-scale topographic effects in acoustic backscatter with small instantaneous insonified areas, caused by ambiguity in the local (beam-to-beam) bed-sonar geometry, are removed. Then, coherent scales between high-resolution topography and backscatter are identified using cospectra, which are used to design a frequency domain filter that decomposes backscatter into the (unwanted) high-pass component associated with bedform topography (ripples, dunes, and sand waves) and vegetation, and the (desired) low-frequency component associated with the composition of sediment patches superimposed on the topography. This process strengthens relationships between backscatter and sediment composition. A probabilistic framework is presented for classifying vegetated and unvegetated substrates based on acoustic backscatter at decimeter resolution. This capability is demonstrated using data collected from diverse settings within a 386 km reach of a canyon river whose bed varies among sand, gravel, cobbles, boulders, and submerged vegetation.

Compositional signatures in acoustic backscatter over vegetated and unvegetated mixed sand-gravel riverbeds

Science.gov (United States)

Buscombe, Daniel; Grams, Paul E.; Kaplinski, Matt A.

2017-01-01

Multibeam acoustic backscatter has considerable utility for remote characterization of spatially heterogeneous bed sediment composition over vegetated and unvegetated riverbeds of mixed sand and gravel. However, the use of high-frequency, decimeter-resolution acoustic backscatter for sediment classification in shallow water is hampered by significant topographic contamination of the signal. In mixed sand-gravel riverbeds, changes in the abiotic composition of sediment (such as homogeneous sand to homogeneous gravel) tend to occur over larger spatial scales than is characteristic of small-scale bedform topography (ripples, dunes, and bars) or biota (such as vascular plants and periphyton). A two-stage method is proposed to filter out the morphological contributions to acoustic backscatter. First, the residual supragrain-scale topographic effects in acoustic backscatter with small instantaneous insonified areas, caused by ambiguity in the local (beam-to-beam) bed-sonar geometry, are removed. Then, coherent scales between high-resolution topography and backscatter are identified using cospectra, which are used to design a frequency domain filter that decomposes backscatter into the (unwanted) high-pass component associated with bedform topography (ripples, dunes, and sand waves) and vegetation, and the (desired) low-frequency component associated with the composition of sediment patches superimposed on the topography. This process strengthens relationships between backscatter and sediment composition. A probabilistic framework is presented for classifying vegetated and unvegetated substrates based on acoustic backscatter at decimeter resolution. This capability is demonstrated using data collected from diverse settings within a 386 km reach of a canyon river whose bed varies among sand, gravel, cobbles, boulders, and submerged vegetation.

Preparation by irradiation of a solid support for enzyme immunoassay

International Nuclear Information System (INIS)

Kumakura, M.; Kaetsu, I.

1984-01-01

Reagents (immobilized anti-α-fetoprotein discs) having a porous structure were prepared for enzyme immunoassay of α-fetoprotein by radiation polymerization at low temperatures. Discs were attached to sticks for easy handling. The activity (determined by absorbance at 492 nm) of the discs varied with the hydrophilic properties and size of the discs. The discs are sufficiently sensitive and precise for enzyme immunoassay of α-fetoprotein. Anti-AFP dissolved in PBS solution was mixed with a monomer solution of hydroxyethyl methacrylate and hydroxypropyl methacrylate. The mixture was frozen to -78 0 C and gamma irradiated. (Auth.)

Backscatter, anisotropy, and polarization of solar hard X-rays

International Nuclear Information System (INIS)

Bai, T.; Ramaty, R.

1978-01-01

Hard X-rays incident upon the photosphere with energies > or approx. =15 keV have high probabilities of backscatter due to Compton collisions with electrons. This effect has a strong influence on the spectrum, intensity, and polarization of solar hard X-rays - especially for anisotropic models in which the primary X-rays are emitted predominantly toward the photosphere. We have carried out a detailed study of X-ray backscatter, and we have investigated the interrelated problems of anisotropy, polarization, center-to-limb variation of the X-ray spectrum, and Compton backscatter in a coherent fashion. The results of this study are compared with observational data. Because of the large contribution from backscatter, for an anisotropic primary X-ray source which is due to bremsstrahlung of accelerated electrons moving predominantly down toward the photosphere, the observed X-ray flux around 30 keV does not depend significantly on the position of flare on the Sun. For such an anisotropic source, the X-ray spectrum observed in the 15-50 keV range becomes steeper with the increasing heliocentric angle of the flare. These results are compatible with the data. The degree of polarization of the sum of the primary and reflected X-rays with energies between about 15 and 30 keV can be very large for anisotropic primary X-ray sources, but it is less than about 4% for isotropic sources. We also discuss the characteristics of the brightness distribution of the X-ray albedo patch created by the Compton backscatter. The height and anisotropy of the primary hard X-ray source might be inferred from the study of the albedo patch

The relationship between VHF radar auroral backscatter amplitude and Doppler velocity: a statistical study

Directory of Open Access Journals (Sweden)

B. A. Shand

Full Text Available A statistical investigation of the relationship between VHF radar auroral backscatter intensity and Doppler velocity has been undertaken with data collected from 8 years operation of the Wick site of the Sweden And Britain Radar-auroral Experiment (SABRE. The results indicate three different regimes within the statistical data set; firstly, for Doppler velocities <200 m s^–1, the backscatter intensity (measured in decibels remains relatively constant. Secondly, a linear relationship is observed between the backscatter intensity (in decibels and Doppler velocity for velocities between 200 m s^–1 and 700 m s^–1. At velocities greater than 700 m s^–1 the backscatter intensity saturates at a maximum value as the Doppler velocity increases. There are three possible geophysical mechanisms for the saturation in the backscatter intensity at high phase speeds: a saturation in the irregularity turbulence level, a maximisation of the scattering volume, and a modification of the local ambient electron density. There is also a difference in the dependence of the backscatter intensity on Doppler velocity for the flow towards and away from the radar. The results for flow towards the radar exhibit a consistent relationship between backscatter intensity and measured velocities throughout the solar cycle. For flow away from the radar, however, the relationship between backscatter intensity and Doppler velocity varies during the solar cycle. The geometry of the SABRE system ensures that flow towards the radar is predominantly associated with the eastward electrojet, and flow away is associated with the westward electrojet. The difference in the backscatter intensity variation as a function of Doppler velocity is attributed to asymmetries between the eastward and westward electrojets and the geophysical parameters controlling the backscatter amplitude.

The relationship between VHF radar auroral backscatter amplitude and Doppler velocity: a statistical study

Directory of Open Access Journals (Sweden)

B. A. Shand

1996-08-01

Full Text Available A statistical investigation of the relationship between VHF radar auroral backscatter intensity and Doppler velocity has been undertaken with data collected from 8 years operation of the Wick site of the Sweden And Britain Radar-auroral Experiment (SABRE. The results indicate three different regimes within the statistical data set; firstly, for Doppler velocities <200 m s–1, the backscatter intensity (measured in decibels remains relatively constant. Secondly, a linear relationship is observed between the backscatter intensity (in decibels and Doppler velocity for velocities between 200 m s–1 and 700 m s–1. At velocities greater than 700 m s–1 the backscatter intensity saturates at a maximum value as the Doppler velocity increases. There are three possible geophysical mechanisms for the saturation in the backscatter intensity at high phase speeds: a saturation in the irregularity turbulence level, a maximisation of the scattering volume, and a modification of the local ambient electron density. There is also a difference in the dependence of the backscatter intensity on Doppler velocity for the flow towards and away from the radar. The results for flow towards the radar exhibit a consistent relationship between backscatter intensity and measured velocities throughout the solar cycle. For flow away from the radar, however, the relationship between backscatter intensity and Doppler velocity varies during the solar cycle. The geometry of the SABRE system ensures that flow towards the radar is predominantly associated with the eastward electrojet, and flow away is associated with the westward electrojet. The difference in the backscatter intensity variation as a function of Doppler velocity is attributed to asymmetries between the eastward and westward electrojets and the geophysical parameters controlling the backscatter amplitude.

Ultrasonic characterization of cancellous bone using apparent integrated backscatter

Energy Technology Data Exchange (ETDEWEB)

Hoffmeister, B K [Department of Physics, Rhodes College, 2000 North Parkway, Memphis, TN 38112 (United States); III, C I Jones [Department of Physics, Rhodes College, 2000 North Parkway, Memphis, TN 38112 (United States); Caldwell, G J [Department of Physics, Rhodes College, 2000 North Parkway, Memphis, TN 38112 (United States); Kaste, S C [Department of Diagnostic Imaging, St Jude Children' s Research Hospital, Memphis, TN 38105 (United States)

2006-06-07

Apparent integrated backscatter (AIB) is a measure of the frequency-averaged (integrated) backscattered power contained in some portion of a backscattered ultrasonic signal. AIB has been used extensively to study soft tissues, but its usefulness as a tissue characterization technique for cancellous bone has not been demonstrated. To address this, we performed measurements on 17 specimens of cancellous bone over two different frequency ranges using a 1 MHz and 5 MHz broadband ultrasonic transducer. Specimens were obtained from bovine tibiae and prepared in the shape of cubes (15 mm side length) with faces oriented along transverse (anterior, posterior, medial and lateral) and longitudinal (superior and inferior) principal anatomic directions. A mechanical scanning system was used to acquire multiple backscatter signals from each direction for each cube. AIB demonstrated highly significant linear correlations with bone mineral density (BMD) for both the transverse (R{sup 2} = 0.817) and longitudinal (R{sup 2} = 0.488) directions using the 5 MHz transducer. In contrast, the correlations with density were much weaker for the 1 MHz transducer (R{sup 2} = 0.007 transverse, R{sup 2} = 0.228 longitudinal). In all cases where a significant correlation was observed, AIB was found to decrease with increasing BMD.

Effects of Biota on Backscatter: Experiments with the Portable Acoustic Laboratory (PAL)

National Research Council Canada - National Science Library

Jones, Christopher

2003-01-01

.... Monitoring showed a sudden drop in backscatter, but this was short lived. Abundant mysid shrimp appeared to have caused microtopographic changes that quickly raised backscatter intensity to near-background levels...

Correlation and uncertainties evaluation in backscattering of entrance surface air kerma measurements

Energy Technology Data Exchange (ETDEWEB)

Teixeira, G.J.; Sousa, C.H.S.; Peixoto, J.G.P., E-mail: gt@ird.gov.br [Instituto de Radioproteção e Dosimetria (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil)

2017-07-01

The air kerma measurement is important to verify the applied doses in radiodiagnostic. The literature determines some methods to measure the entrance surface air kerma or entrance surface dose but some of this methods may increase the measurement with the backscattering. Were done setups of measurements to do correlations between them. The expanded uncertainty exceeded 5% for measurements with backscattering, reaching 8.36%, while in situations where the backscattering was avoided, the uncertainty was 3.43%. (author)

Novel immunoassay formats for integrated microfluidic circuits: diffusion immunoassays (DIA)

Science.gov (United States)

Weigl, Bernhard H.; Hatch, Anson; Kamholz, Andrew E.; Yager, Paul

2000-03-01

Novel designs of integrated fluidic microchips allow separations, chemical reactions, and calibration-free analytical measurements to be performed directly in very small quantities of complex samples such as whole blood and contaminated environmental samples. This technology lends itself to applications such as clinical diagnostics, including tumor marker screening, and environmental sensing in remote locations. Lab-on-a-Chip based systems offer many *advantages over traditional analytical devices: They consume extremely low volumes of both samples and reagents. Each chip is inexpensive and small. The sampling-to-result time is extremely short. They perform all analytical functions, including sampling, sample pretreatment, separation, dilution, and mixing steps, chemical reactions, and detection in an integrated microfluidic circuit. Lab-on-a-Chip systems enable the design of small, portable, rugged, low-cost, easy to use, yet extremely versatile and capable diagnostic instruments. In addition, fluids flowing in microchannels exhibit unique characteristics ('microfluidics'), which allow the design of analytical devices and assay formats that would not function on a macroscale. Existing Lab-on-a-chip technologies work very well for highly predictable and homogeneous samples common in genetic testing and drug discovery processes. One of the biggest challenges for current Labs-on-a-chip, however, is to perform analysis in the presence of the complexity and heterogeneity of actual samples such as whole blood or contaminated environmental samples. Micronics has developed a variety of Lab-on-a-Chip assays that can overcome those shortcomings. We will now present various types of novel Lab- on-a-Chip-based immunoassays, including the so-called Diffusion Immunoassays (DIA) that are based on the competitive laminar diffusion of analyte molecules and tracer molecules into a region of the chip containing antibodies that target the analyte molecules. Advantages of this

Nitrocellulose membrane-based enzyme-linked immunoassay for dengue serotype-1 IgM detection

International Nuclear Information System (INIS)

Leon, S.; Guevara, C.; Chunga, A.

1999-01-01

To evaluate the sensitivity and specifity of a nitrocellulose membrane-based immunoassay for dengue IgM, with respect to capture enzyme immunoassay, for the diagnosis of dengue virus infection. 101 serum samples were processed and divided into 2 groups: 53 from dengue serotype 1 (DEN1) infected patients, and 48 from healthy subjects. Both groups were tested with a nitrocellulose membrane-based IgM capture enzyme immunoassay (NMB-EIA) and also with an ELISA as referential pattern. NMB-EIA testing detected IgM anti-DEN1 in 94,34% of samples from infected patients, and in 14,58% of control samples, whereas ELISA fails to report false positive or false negative results: NMB-EIA appears to be a good alternative for dengue infection diagnosis. (authors)

Rapid detection of fungal alpha-amylase in the work environment with a lateral flow immunoassay

NARCIS (Netherlands)

Bogdanovic, J.; Koets, M.; Sander, I.; Wouters, I.; Meijster, T.; Heederik, D.J.J.; Amerongen, van A.; Doekes, G.

2006-01-01

Background Occupational allergen exposure assessment usually requires airborne dust sampling at the worksite followed by dust extraction and enzyme immunoassay (EIA) analysis at the laboratory. Use of semiquantitative lateral flow immunoassays (LFIAs) may allow a more rapid detection procedure with

A framework to quantify uncertainties of seafloor backscatter from swath mapping echosounders

Science.gov (United States)

Malik, Mashkoor; Lurton, Xavier; Mayer, Larry

2018-06-01

Multibeam echosounders (MBES) have become a widely used acoustic remote sensing tool to map and study the seafloor, providing co-located bathymetry and seafloor backscatter. Although the uncertainty associated with MBES-derived bathymetric data has been studied extensively, the question of backscatter uncertainty has been addressed only minimally and hinders the quantitative use of MBES seafloor backscatter. This paper explores approaches to identifying uncertainty sources associated with MBES-derived backscatter measurements. The major sources of uncertainty are catalogued and the magnitudes of their relative contributions to the backscatter uncertainty budget are evaluated. These major uncertainty sources include seafloor insonified area (1-3 dB), absorption coefficient (up to > 6 dB), random fluctuations in echo level (5.5 dB for a Rayleigh distribution), and sonar calibration (device dependent). The magnitudes of these uncertainty sources vary based on how these effects are compensated for during data acquisition and processing. Various cases (no compensation, partial compensation and full compensation) for seafloor insonified area, transmission losses and random fluctuations were modeled to estimate their uncertainties in different scenarios. Uncertainty related to the seafloor insonified area can be reduced significantly by accounting for seafloor slope during backscatter processing while transmission losses can be constrained by collecting full water column absorption coefficient profiles (temperature and salinity profiles). To reduce random fluctuations to below 1 dB, at least 20 samples are recommended to be used while computing mean values. The estimation of uncertainty in backscatter measurements is constrained by the fact that not all instrumental components are characterized and documented sufficiently for commercially available MBES. Further involvement from manufacturers in providing this essential information is critically required.

Modification of diode characteristics by electron back-scatter from high-atomic-number anodes

International Nuclear Information System (INIS)

Mosher, D.; Cooperstein, G.; Rose, D.V.; Swanekamp, S.B.

1996-01-01

In high-power vacuum diodes with high-atomic-number anodes, back-scattered electrons alter the vacuum space charge and resulting electron and ion currents. Electron multiple back-scattering was studied through equilibrium solutions of the Poisson equation for 1-dimensional, bipolar diodes in order to predict their early-time behavior. Before ion turn-on, back-scattered electrons from high-Z anodes suppress the diode current by about 10%. After ion turn-on in the same diodes, electron back-scatter leads to substantial enhancements of both the electron and ion currents above the Child-Langmuir values. Current enhancements with ion flow from low-Z anodes are small. (author). 5 figs., 7 refs

Modification of diode characteristics by electron back-scatter from high-atomic-number anodes

Energy Technology Data Exchange (ETDEWEB)

Mosher, D; Cooperstein, G [Naval Research Laboratory, Washington, DC (United States); Rose, D V; Swanekamp, S B [JAYCOR, Vienna, VA (United States)

1997-12-31

In high-power vacuum diodes with high-atomic-number anodes, back-scattered electrons alter the vacuum space charge and resulting electron and ion currents. Electron multiple back-scattering was studied through equilibrium solutions of the Poisson equation for 1-dimensional, bipolar diodes in order to predict their early-time behavior. Before ion turn-on, back-scattered electrons from high-Z anodes suppress the diode current by about 10%. After ion turn-on in the same diodes, electron back-scatter leads to substantial enhancements of both the electron and ion currents above the Child-Langmuir values. Current enhancements with ion flow from low-Z anodes are small. (author). 5 figs., 7 refs.

Immunoassay separation technique

International Nuclear Information System (INIS)

1977-01-01

A method for effecting the immunoassay of a multiplicity of samples, each possibly containing an antigen or an antibody to be assayed, is discussed. Each sample is incubated with a solution containing a detectable antigen or antibody to form a multiplicity of mixtures, each mixture containing as components antigen-antibody, non-complexed antigen and non-complexed antibody. At least one of the components of the said mixture is separated by adsorption. There after, quantity of detectable antigen or antibody is detected in one of the non-adsorbed portions of the mixture. An improvement, compared to other techniques, is the continuous and sequential separation of at least one component, which is intended to be separated from each said multiplicity of mixtures

Elastic and inelastic surface effects on ion penetration and the resulting sputtering and backscattering

International Nuclear Information System (INIS)

Hassanein, A.M.; Smith, D.L.

1985-01-01

The computer code ITMC (Ion Transport in Materials and Compounds) has been developed to study in detail the transport of charged particles in solid materials and surface related phenomena such as sputtered atoms and backscattered ions. The code is based on Monte Carlo methods to follow the path and the damage produced by the charged particles in three dimension as they slow down in target materials. Single-element targets as well as alloys with possible different surface and bulk compositions or with layered structures of different materials can be used. Various models developed to calculate the inelastic energy losses with target electrons can be used in the code. Most known interatomic potentials can also be used to calculate the elastic energy losses. The major advantages of the code are its ability and flexibility to use and compare various models of elastic and inelastic energy losses in any target with different compounds and different surface and bulk composition

Optical diagnostics of CO2 laser-fusion targets using backscattered light

International Nuclear Information System (INIS)

Casperson, D.E.

1981-01-01

With the f/2.4 focusing optics on one of the eight Helios CO 2 laser beam lines, direct backscattered light from a variety of glass microballoon targets has been observed. The quantities that have been measured include: (1) the total backscattered energy; (2) relative amplitudes of the backscattered fundamental and low harmonics (n = 1, 2, 3) of the 10.6 μm incident light; (3) the 3/2 harmonic emission from a double pulse backscatter experiment; (4) the temporally resolved 10.6 μm light using a fast pyroelectric detector and a Los Alamos 5-GHz oscilloscope; and (5) the time-integrated spectrally resolved fundamental using a 3/4 meter spectrometer and a high resolution pyroelectric detector array (resolution approx. 40 A at 10.6 μm). The suitability of these diagnostics for evaluating the CO 2 laser plasma in terms of stimulated scattering processes, plasma density gradients, velocity of the critical surface, etc., is discussed

Principles of electron backscattering by solids and thin films

International Nuclear Information System (INIS)

Niedrig, H.

1977-01-01

The parameters concerning the electron backscattering from thin films and solids (atomic scattering cross-section, atomic number, single/multiple scattering, film thickness of self-supporting films and of surface films on bulk substrates, scattering angular distribution, angle of incidence, diffraction effects) are described. Their influence on some important contrast mechanisms in scanning electron microscopy (thickness contrast, Z/material contrast, tilting/topography contrast, orientation contrast) is discussed. The main backscattering electron detection systems are briefly described. (orig.) [de

Comparison of pre-processing methods for multiplex bead-based immunoassays.

Science.gov (United States)

Rausch, Tanja K; Schillert, Arne; Ziegler, Andreas; Lüking, Angelika; Zucht, Hans-Dieter; Schulz-Knappe, Peter

2016-08-11

High throughput protein expression studies can be performed using bead-based protein immunoassays, such as the Luminex® xMAP® technology. Technical variability is inherent to these experiments and may lead to systematic bias and reduced power. To reduce technical variability, data pre-processing is performed. However, no recommendations exist for the pre-processing of Luminex® xMAP® data. We compared 37 different data pre-processing combinations of transformation and normalization methods in 42 samples on 384 analytes obtained from a multiplex immunoassay based on the Luminex® xMAP® technology. We evaluated the performance of each pre-processing approach with 6 different performance criteria. Three performance criteria were plots. All plots were evaluated by 15 independent and blinded readers. Four different combinations of transformation and normalization methods performed well as pre-processing procedure for this bead-based protein immunoassay. The following combinations of transformation and normalization were suitable for pre-processing Luminex® xMAP® data in this study: weighted Box-Cox followed by quantile or robust spline normalization (rsn), asinh transformation followed by loess normalization and Box-Cox followed by rsn.

The studies of radiation distorations in CdS single crystals by using a proton back-scattering method

International Nuclear Information System (INIS)

Grigor'ev, A.N.; Dikij, N.P.; Matyash, P.P.; Nikolajchuk, L.I.; Pivovar, L.I.

1974-01-01

The radiation defects in semiconducting CdS single crystals induced during doping with 140 keV Na ions (10 15 -2.10 16 ion/cm 2 ) were studied by the orientation dependence of 700 keV proton backscattering. The absence of discrete peaks in the scattered proton eneryg spectra indicates a small contribution of direct scattering at large angles. The defects formed during doping increase the fractionof dechanneled particles, which are then scattered at large anlges. No amorphization of CdS was observed at high Na ion dose 2x10 16 ion/cm 2

Distributed Sensor Particles for Remote Fluorescence Detection of Trace Analytes: UXO/CW; TOPICAL

International Nuclear Information System (INIS)

SINGH, ANUP K.; GUPTA, ALOK; MULCHANDANI, ASHOK; CHEN, WILFRED; BHATIA, RIMPLE B.; SCHOENIGER, JOSEPH S.; ASHLEY, CAROL S.; BRINKER, C. JEFFREY; HANCE, BRADLEY G.; SCHMITT, RANDAL L.; JOHNSON, MARK S.; HARGIS JR. PHILIP J.; SIMONSON, ROBERT J.

2001-01-01

This report summarizes the development of sensor particles for remote detection of trace chemical analytes over broad areas, e.g residual trinitrotoluene from buried landmines or other unexploded ordnance (UXO). We also describe the potential of the sensor particle approach for the detection of chemical warfare (CW) agents. The primary goal of this work has been the development of sensor particles that incorporate sample preconcentration, analyte molecular recognition, chemical signal amplification, and fluorescence signal transduction within a ''grain of sand''. Two approaches for particle-based chemical-to-fluorescence signal transduction are described: (1) enzyme-amplified immunoassays using biocompatible inorganic encapsulants, and (2) oxidative quenching of a unique fluorescent polymer by TNT

Spectral dependence of backscattering coefficient of mixed phase clouds over West Africa measured with two-wavelength Raman polarization lidar: Features attributed to ice-crystals corner reflection

Science.gov (United States)

Veselovskii, I.; Goloub, P.; Podvin, T.; Tanre, D.; Ansmann, A.; Korenskiy, M.; Borovoi, A.; Hu, Q.; Whiteman, D. N.

2017-11-01

The existing models predict that corner reflection (CR) of laser radiation by simple ice crystals of perfect shape, such as hexagonal columns or plates, can provide a significant contribution to the ice cloud backscattering. However in real clouds the CR effect may be suppressed due to crystal deformation and surface roughness. In contrast to the extinction coefficient, which is spectrally independent, consideration of diffraction associated with CR results in a spectral dependence of the backscattering coefficient. Thus measuring the spectral dependence of the cloud backscattering coefficient, the contribution of CR can be identified. The paper presents the results of profiling of backscattering coefficient (β) and particle depolarization ratio (δ) of ice and mixed-phase clouds over West Africa by means of a two-wavelength polarization Mie-Raman lidar operated at 355 nm and 532 nm during the SHADOW field campaign. The lidar observations were performed at a slant angle of 43 degree off zenith, thus CR from both randomly oriented crystals and oriented plates could be analyzed. For the most of the observations the cloud backscatter color ratio β355/β532 was close to 1.0, and no spectral features that might indicate the presence of CR of randomly oriented crystals were revealed. Still, in two measurement sessions we observed an increase of backscatter color ratio to a value of nearly 1.3 simultaneously with a decrease of the spectral depolarization ratio δ355/δ532 ratio from 1.0 to 0.8 inside the layers containing precipitating ice crystals. We attribute these changes in optical properties to corner reflections by horizontally oriented ice plates.

Characterizing Indian Ocean manganese nodule-bearing seafloor using multi-beam angular backscatter

Digital Repository Service at National Institute of Oceanography (India)

Chakraborty, B.; Kodagali, V.N.

backscattering in delineating seafloor parameters characteristic of nodule-rich sediments. In this paper, processed Hydrosweep multi-beam backscatter data from 45 spot locations in the CIOB (where nodule samples are available) were analysed to estimate seafloor...

The experimental method of measurement for spatial distribution of full aperture backscatter light by circular PIN-array

International Nuclear Information System (INIS)

Zhao Xuefeng; Wang Chuanke; Hu Feng; Kuang Longyu; Wang Zhebin; Li Sanwei; Liu Shengye; Jiang Gang

2011-01-01

The spatial distribution of backscatter light is very important for understanding the production of backscatter light. The experimental method of spatial distribution of full aperture backscatter light is based on the circular PIN array composed of concentric orbicular multi-PIN detectors. The image of backscatter light spatial distribution of full aperture SBS is obtained by measuring spatial distribution of full aperture backscatter light using the method in the experiment of laser hohlraum targets interaction at 'Shenguang II'. A preliminary method to measure spatial distribution of full aperture backscatter light is established. (authors)

Feasibility of a simple microsieve-based immunoassay platform

NARCIS (Netherlands)

Zweitzig, D.R.; Tibbe, Arjan G.J.; Nguyen, A.T.; van Rijn, C.J.M.; Kopnitsky, M.J.; Cichonski, K.; Terstappen, Leonardus Wendelinus Mathias Marie

2016-01-01

The intrinsic properties of silicon microsieves, such as an optically flat surface, high overall porosity, and low flow resistance have led to an increasing number of biotechnology applications. In this report, the feasibility of creating a microsieve-based immunoassay platform was explored.

Feasibility of a simple microsieve-based immunoassay platform

NARCIS (Netherlands)

Zweitzig, Daniel R.; Tibbe, Arjan G.; Nguyen, Ai T.; Rijn, van Cees J.M.; Kopnitsky, Mark J.; Cichonski, Kathleen; Terstappen, Leon W.M.M.

2016-01-01

The intrinsic properties of silicon microsieves, such as an optically flat surface, high overall porosity, and low flow resistance have led to an increasing number of biotechnology applications. In this report, the feasibility of creating a microsieve-based immunoassay platform was explored.

Alkaline phosphatase-fused repebody as a new format of immuno-reagent for an immunoassay

Energy Technology Data Exchange (ETDEWEB)

Seo, Hyo-Deok; Lee, Joong-jae [Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701 (Korea, Republic of); Kim, Yu Jung [Industrial Biotechnology and Bioenergy Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon (Korea, Republic of); Hantschel, Oliver [School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne (Switzerland); Lee, Seung-Goo [Industrial Biotechnology and Bioenergy Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon (Korea, Republic of); Kim, Hak-Sung, E-mail: hskim76@kaist.ac.kr [Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701 (Korea, Republic of)

2017-01-15

Enzyme-linked immunoassays based on an antibody-antigen interaction are widely used in biological and medical sciences. However, the conjugation of an enzyme to antibodies needs an additional chemical process, usually resulting in randomly cross-linked molecules and a loss of the binding affinity and enzyme activity. Herein, we present the development of an alkaline phosphatase-fused repebody as a new format of immuno-reagent for immunoassays. A repebody specifically binding to human TNF-α (hTNF-α) was selected through a phage display, and its binding affinity was increased up to 49 nM using a modular engineering approach. A monomeric alkaline phosphatase (mAP), which was previously isolated from a metagenome library, was genetically fused to the repebody as a signal generator, and the resulting repebody-mAP fusion protein was used for direct and sandwich immunoassays of hTNF-α. We demonstrate the utility and potential of the repebody-mAP fusion protein as an immuno-reagent by showing the sensitivity of 216 pg mL{sup −1} for hTNF-α in a sandwich immunoassay. Furthermore, this repebody-mAP fusion protein enabled the detection of hTNF-α spiked in a serum-supplemented medium with high accuracy and reproducibility. It is thus expected that a mAP-fused repebody can be broadly used as an immuno-reagent in immunoassays. - Highlights: • A human TNF-α (hTNF-α)-specific repebody was selected using a phage display. • A monomeric alkaline phosphatase (mAP) was genetically fused to the repebody. • mAP-fused repebody enabled detection of hTNF-α with high sensitivity and accuracy. • mAP-fused repebody can be widely used as a new immuno-reagent in immunoassays.

Study on the determination of human placental lactogen (HPL) using an enzyme-immunoassay. Comparison with a commercial radio-immunoassay in the course of normal pregnancies

International Nuclear Information System (INIS)

Schneider, B.

1982-01-01

A novel enzyme-immunoassay (EIA) for determining human placental lactogen (HPL) was studied for its practicability and quality. The precision of the system in series was tested by using a serum taken each in the 19th, 29th and 40th pregnancy week. A normal range graph between the 10th and the 40th pregnancy week (10 sera per pregnancy week) was established from 310 sera of normal-course pregnancies. The graph practically agreed with the known RIA-established graphs. When comparing with a radio-immunoassay for HPL of routine application and known quality criteria, r=0.93 indicated a close correlation of the values found. (orig./MG) [de

Surface-Enhanced Raman Scattering (SERS) for Detection in Immunoassays: applications, fundamentals, and optimization

International Nuclear Information System (INIS)

Jeremy Daniel Driskell

2006-01-01

Immunoassays have been utilized for the detection of biological analytes for several decades. Many formats and detection strategies have been explored, each having unique advantages and disadvantages. More recently, surface-enhanced Raman scattering (SERS) has been introduced as a readout method for immunoassays, and has shown great potential to meet many key analytical figures of merit. This technology is in its infancy and this dissertation explores the diversity of this method as well as the mechanism responsible for surface enhancement. Approaches to reduce assay times are also investigated. Implementing the knowledge gained from these studies will lead to a more sensitive immunoassay requiring less time than its predecessors. This dissertation is organized into six sections. The first section includes a literature review of the previous work that led to this dissertation. A general overview of the different approaches to immunoassays is given, outlining the strengths and weaknesses of each. Included is a detailed review of binding kinetics, which is central for decreasing assay times. Next, the theoretical underpinnings of SERS is reviewed at its current level of understanding. Past work has argued that surface plasmon resonance (SPR) of the enhancing substrate influences the SERS signal; therefore, the SPR of the extrinsic Raman labels (ERLs) utilized in our SERS-based immunoassay is discussed. Four original research chapters follow the Introduction, each presented as separate manuscripts. Chapter 2 modifies a SERS-based immunoassay previously developed in our group, extending it to the low-level detection of viral pathogens and demonstrating its versatility in terms of analyte type, Chapter 3 investigates the influence of ERL size, material composition, and separation distance between the ERLs and capture substrate on the SERS signal. This chapter links SPR with SERS enhancement factors and is consistent with many of the results from theoretical treatments

Surface-Enhanced Raman Scattering (SERS) for Detection in Immunoassays. Applications, fundamentals, and optimization

Energy Technology Data Exchange (ETDEWEB)

Driskell, Jeremy Daniel [Iowa State Univ., Ames, IA (United States)

2006-08-09

Immunoassays have been utilized for the detection of biological analytes for several decades. Many formats and detection strategies have been explored, each having unique advantages and disadvantages. More recently, surface-enhanced Raman scattering (SERS) has been introduced as a readout method for immunoassays, and has shown great potential to meet many key analytical figures of merit. This technology is in its infancy and this dissertation explores the diversity of this method as well as the mechanism responsible for surface enhancement. Approaches to reduce assay times are also investigated. Implementing the knowledge gained from these studies will lead to a more sensitive immunoassay requiring less time than its predecessors. This dissertation is organized into six sections. The first section includes a literature review of the previous work that led to this dissertation. A general overview of the different approaches to immunoassays is given, outlining the strengths and weaknesses of each. Included is a detailed review of binding kinetics, which is central for decreasing assay times. Next, the theoretical underpinnings of SERS is reviewed at its current level of understanding. Past work has argued that surface plasmon resonance (SPR) of the enhancing substrate influences the SERS signal; therefore, the SPR of the extrinsic Raman labels (ERLs) utilized in our SERS-based immunoassay is discussed. Four original research chapters follow the Introduction, each presented as separate manuscripts. Chapter 2 modifies a SERS-based immunoassay previously developed in our group, extending it to the low-level detection of viral pathogens and demonstrating its versatility in terms of analyte type, Chapter 3 investigates the influence of ERL size, material composition, and separation distance between the ERLs and capture substrate on the SERS signal. This chapter links SPR with SERS enhancement factors and is consistent with many of the results from theoretical treatments

Multi-temporal RADARSAT-1 and ERS backscattering signatures of coastal wetlands in southeastern Louisiana

Science.gov (United States)

Kwoun, Oh-Ig; Lu, Z.

2009-01-01

Using multi-temporal European Remote-sensing Satellites (ERS-1/-2) and Canadian Radar Satellite (RADARSAT-1) synthetic aperture radar (SAR) data over the Louisiana coastal zone, we characterize seasonal variations of radar backscat-tering according to vegetation type. Our main findings are as follows. First, ERS-1/-2 and RADARSAT-1 require careful radiometric calibration to perform multi-temporal backscattering analysis for wetland mapping. We use SAR backscattering signals from cities for the relative calibration. Second, using seasonally averaged backscattering coefficients from ERS-1/-2 and RADARSAT-1, we can differentiate most forests (bottomland and swamp forests) and marshes (freshwater, intermediate, brackish, and saline marshes) in coastal wetlands. The student t-test results support the usefulness of season-averaged backscatter data for classification. Third, combining SAR backscattering coefficients and an optical-sensor-based normalized difference vegetation index can provide further insight into vegetation type and enhance the separation between forests and marshes. Our study demonstrates that SAR can provide necessary information to characterize coastal wetlands and monitor their changes.

Effect of species structure and dielectric constant on C-band forest backscatter

Science.gov (United States)

Lang, R. H.; Landry, R.; Kilic, O.; Chauhan, N.; Khadr, N.; Leckie, D.

1993-01-01

A joint experiment between Canadian and USA research teams was conducted early in Oct. 1992 to determine the effect of species structure and dielectric variations on forest backscatter. Two stands, one red pine and one jack pine, in the Petawawa National Forestry Institute (PNFI) were utilized for the experiment. Extensive tree architecture measurements had been taken by the Canada Centre for Remote Sensing (CCRS) several months earlier by employing a Total Station surveying instrument which provides detailed information on branch structure. A second part of the experiment consisted of cutting down several trees and using dielectric probes to measure branch and needle permittivity values at both sites. The dielectric and the tree geometry data were used in the George Washington University (GWU) Vegetation Model to determine the C band backscattering coefficients of the individual stands for VV polarization. The model results show that backscatter at C band comes mainly from the needles and small branches and the upper portion of the trunks acts only as an attenuator. A discussion of variation of backscatter with specie structure and how dielectric variations in needles for both species may affect the total backscatter returns is provided.

Current status and future developments in radiolabelled immunoassays

International Nuclear Information System (INIS)

Edwards, R.

1998-01-01

Radioisotopes are used extensively in medical practice and their use in RIA or IRMA usually represent a small proportion of the total. Radiolabelled immunoassays based on 125 I constitute a simple didactic, cost effective and robust technology which is still regarded as the reference method in many clinical applications. The IAEA has implemented many successful programmes using the ''bulk reagent'' approach, involving 68 countries in all the different regions. The main achievements have been in technology transfer with self sufficiency in production for some countries; training of large numbers of staff; quality control and quality assurance schemes; devolution of screening programmes for neonatal congenital hypothryoidism. Alternatives to the use of radioisotopic tracers are constrained by many factors and are often only available in restricted commercial packages. They are often not suitable for technology transfer programmes and often lack any didactic component in addition to a relative high cost. The production of radiolabels using 125 I is both simple and adaptable. In addition expertise in their preparation and purification is widespread even in developing countries. Together with the ease of producing antibodies, the facts have made 125 I-radiolabelled immunoassays ideal for investigative procedures for many research activities (30,31) particularly in the medical context where radioisotopes are commonly used. In conclusion, even a superficial examination of public health statistics for various countries throughout the continents indicates a need for a simple, inexpensive and robust analytical tool. In this light, there is a predicted continuing role for radiolabelled immunoassays. (author)

Evaluation of six immunoassays for detection of dengue virus-specific immunoglobulin M and G antibodies

NARCIS (Netherlands)

J. Groen (Jan); P. Koraka (Penelope); J. Velzing (Jans); C. Copra (Cederick); A.D.M.E. Osterhaus (Albert)

2000-01-01

textabstractThe performance of six commercially available immunoassay systems for the detection of dengue virus-specific immunoglobulin M (IgM) and IgG antibodies in serum was evaluated. These included two IgM and IgG enzyme immunoassays (EIA) from MRL Laboratories and PanBio, a rapid

A dedicated beam line for Rutherford backscattering analysis at IFIN-HH cyclotron

International Nuclear Information System (INIS)

Ivanov, E. A.; Dudu, D.; Plostinaru, D.; Catana, D.; Vata, I.

2003-01-01

Rutherford back-scattering technique (RBS) is an analytical tool that uses elastic scattering of 1-5 MeV charged particles for analysis of the surface and the outer few micrometers of solids. IFIN-HH RBS system consists of the U-120 Cyclotron, a dedicated beam line and a scattering chamber with sample manipulators and particle detectors. In our RBS system the samples are bombarded with 2-5 alpha particles accelerated by U-120 Cyclotron (in 3-rd subharmonic regime) while the scattered particles are detected by a surface barrier detector. The signal from the detector is processed by common nuclear electronics and the particle energy spectra are stored in a computer based multichannel analyser. The data evaluation is accomplished using standard procedures and computer codes. The necessary vacuum inside chamber is obtained with an oil-free turbo pump. The beam spot dimension on the target is 1x1 mm. The standard measurement are done at Θ = 165 angle. The samples are electrically insulated and can be rotated around a vertical axis. The advantage of the RBS technique lies in the quantitative analysis of major and minor constituents lying in the first 0.5 to 2.0 micrometers of a material. Depending on the sample structure and composition, the detection limits vary from 10 11 to 10 15 at. cm -2 for heavy and light elements, respectively. The depth distribution of constituents can be reconstructed with a depth resolution of 10-20 nm. The RBS technique is non-destructive since the erosion and the radiation degradation of the sample material by the particle impact is negligible. The most extensive use of the RBS technique is in the field of electronic and optical materials, special coatings and in the study of various physico-chemical processes on the solid surfaces. (authors)

Validation of automated supervised segmentation of multibeam backscatter data from the Chatham Rise, New Zealand

Science.gov (United States)

Hillman, Jess I. T.; Lamarche, Geoffroy; Pallentin, Arne; Pecher, Ingo A.; Gorman, Andrew R.; Schneider von Deimling, Jens

2018-06-01

Using automated supervised segmentation of multibeam backscatter data to delineate seafloor substrates is a relatively novel technique. Low-frequency multibeam echosounders (MBES), such as the 12-kHz EM120, present particular difficulties since the signal can penetrate several metres into the seafloor, depending on substrate type. We present a case study illustrating how a non-targeted dataset may be used to derive information from multibeam backscatter data regarding distribution of substrate types. The results allow us to assess limitations associated with low frequency MBES where sub-bottom layering is present, and test the accuracy of automated supervised segmentation performed using SonarScope® software. This is done through comparison of predicted and observed substrate from backscatter facies-derived classes and substrate data, reinforced using quantitative statistical analysis based on a confusion matrix. We use sediment samples, video transects and sub-bottom profiles acquired on the Chatham Rise, east of New Zealand. Inferences on the substrate types are made using the Generic Seafloor Acoustic Backscatter (GSAB) model, and the extents of the backscatter classes are delineated by automated supervised segmentation. Correlating substrate data to backscatter classes revealed that backscatter amplitude may correspond to lithologies up to 4 m below the seafloor. Our results emphasise several issues related to substrate characterisation using backscatter classification, primarily because the GSAB model does not only relate to grain size and roughness properties of substrate, but also accounts for other parameters that influence backscatter. Better understanding these limitations allows us to derive first-order interpretations of sediment properties from automated supervised segmentation.

A fast universal immobilization of immunoglobulin G at 4 °C for the development of array-based immunoassays.

Directory of Open Access Journals (Sweden)

Shu-Lin Guo

Full Text Available To maintain the antibody activity and enhance performance of array-based immunoassays, protein G was used to allow a shorter duration of immunoglobulin G immobilization at 4 °C, with the antibody placed in the appropriate orientation. The multiplexed detection of six pain-related message molecules (PRMMs was used as examples for the development of array-based immunoassays: substance P, calcitonin gene-related peptide, nerve growth factor, brain-derived neurotrophic factor, tumor necrosis factor-α, and β-endorphin. Protein G- and non-protein G-coated slides were tested. Compared to non-protein G immunoassays, protein G shortened the antibody immobilization time at 4 °C from overnight to 2 hours. Only protein G-facilitated immunoassays succeeded in simultaneously detecting all six PRMMs with high specificity. Dose-response curves showed that the limits of detection of the protein G-multiplexed immunoassays for the PRMMs was approximately 164, 167, 120, 60, 80, and 92 pg/ml, respectively. Thus, protein G effectively shortens the duration of antibody immobilization at 4 °C, allowing the use of sensitive array-based immunoassays for the simultaneous detection of PRMMs.

Contrast and decay of cathodoluminescence from phosphor particles in a scanning electron microscope

Energy Technology Data Exchange (ETDEWEB)

Engelsen, Daniel den; Harris, Paul G.; Ireland, Terry G., E-mail: terry.ireland@brunel.ac.uk; Fern, George R.; Silver, Jack

2015-10-15

Cathodoluminescence (CL) studies are reported on phosphors in a field emission scanning electron microscope (FESEM). ZnO: Zn and other luminescent powders manifest a bright ring around the periphery of the particles: this ring enhances the contrast. Additionally, particles resting on top of others are substantially brighter than underlying ones. These phenomena are explained in terms of the combined effects of electrons backscattered out of the particles, together with light absorption by the substrate. The contrast is found to be a function of the particle size and the energy of the primary electrons. Some phosphor materials exhibit a pronounced comet-like structure at high scan rates in a CL-image, because the particle continues to emit light after the electron beam has moved to a position without phosphor material. Image analysis has been used to study the loss of brightness along the tail and hence to determine the decay time of the materials. The effect of phosphor saturation on the determination of decay times by CL-microscopy was also investigated. - Highlights: • Contrast enhancement are observed in secondary electron and cathodoluminescent images of phosphor particles sitting on top of others. • Backscattered electrons largely explain the observed contrast enhancement. • After glow effects in CL-micrographs of phosphors enable the determination of decay times. • Phosphor saturation can be used to determine the decay time of individual spectral transitions.

Selection Algorithm for the CALIPSO Lidar Aerosol Extinction-to-Backscatter Ratio

Science.gov (United States)

Omar, Ali H.; Winker, David M.; Vaughan, Mark A.

2006-01-01

The extinction-to-backscatter ratio (S(sub a)) is an important parameter used in the determination of the aerosol extinction and subsequently the optical depth from lidar backscatter measurements. We outline the algorithm used to determine Sa for the Cloud and Aerosol Lidar and Infrared Pathfinder Spaceborne Observations (CALIPSO) lidar. S(sub a) for the CALIPSO lidar will either be selected from a look-up table or calculated using the lidar measurements depending on the characteristics of aerosol layer. Whenever suitable lofted layers are encountered, S(sub a) is computed directly from the integrated backscatter and transmittance. In all other cases, the CALIPSO observables: the depolarization ratio, delta, the layer integrated attenuated backscatter, beta, and the mean layer total attenuated color ratio, gamma, together with the surface type, are used to aid in aerosol typing. Once the type is identified, a look-up-table developed primarily from worldwide observations, is used to determine the S(sub a) value. The CALIPSO aerosol models include desert dust, biomass burning, background, polluted continental, polluted dust, and marine aerosols.

An ultrasensitive chemiluminescence immunoassay of chloramphenicol based on gold nanoparticles and magnetic beads.

Science.gov (United States)

Tao, Xiaoqi; Jiang, Haiyang; Yu, Xuezhi; Zhu, Jinghui; Wang, Xia; Wang, Zhanhui; Niu, Lanlan; Wu, Xiaoping; Shen, Jianzhong

2013-05-01

A competitive, direct, chemiluminescent immunoassay based on a magnetic beads (MBs) separation and gold nanoparticles (AuNPs) labelling technique to detect chloramphenicol (CAP) has been developed. Horseradish peroxidase (HRP)-labelled anti-CAP monoclonal antibody conjugated with AuNPs and antigen-immobilized MBs were prepared. After optimization parameters of immunocomplex MBs, the IC50 values of chemiluminescence magnetic nanoparticles immunoassay (CL-MBs-nano-immunoassay) were 0.017 µg L(-1) for extract method I and 0.17 µg L(-1) for extract method II. The immunoassay with two extract methods was applied to detect CAP in milk. Comparison of these two extract methods showed that extract method I was advantageous in better sensitivity, in which the sensitivity was 10 times compared to that of extract method II, while extract method II was superior in simple operation, suitable for high throughout screen. The recoveries were 86.7-98.0% (extract method I) and 80.0-103.0% (extract method II), and the coefficients of variation (CVs) were all recovery with both extract methods and high correlation with traditional ELISA kit in milk system confirmed that the immunomagnetic assay based on AuNPs exhibited promising potential in rapid field screening for trace CAP analysis. Copyright © 2013 John Wiley & Sons, Ltd.

Simulation of multistatic and backscattering cross sections for airborne radar

Science.gov (United States)

Biggs, Albert W.

1986-07-01

In order to determine susceptibilities of airborne radar to electronic countermeasures and electronic counter-countermeasures simulations of multistatic and backscattering cross sections were developed as digital modules in the form of algorithms. Cross section algorithms are described for prolate (cigar shape) and oblate (disk shape) spheroids. Backscattering cross section algorithms are also described for different categories of terrain. Backscattering cross section computer programs were written for terrain categorized as vegetation, sea ice, glacial ice, geological (rocks, sand, hills, etc.), oceans, man-made structures, and water bodies. PROGRAM SIGTERRA is a file for backscattering cross section modules of terrain (TERRA) such as vegetation (AGCROP), oceans (OCEAN), Arctic sea ice (SEAICE), glacial snow (GLASNO), geological structures (GEOL), man-made structures (MAMMAD), or water bodies (WATER). AGCROP describes agricultural crops, trees or forests, prairies or grassland, and shrubs or bush cover. OCEAN has the SLAR or SAR looking downwind, upwind, and crosswind at the ocean surface. SEAICE looks at winter ice and old or polar ice. GLASNO is divided into a glacial ice and snow or snowfields. MANMAD includes buildings, houses, roads, railroad tracks, airfields and hangars, telephone and power lines, barges, trucks, trains, and automobiles. WATER has lakes, rivers, canals, and swamps. PROGRAM SIGAIR is a similar file for airborne targets such as prolate and oblate spheroids.

Cavitation inception by the backscattering of pressure waves from a bubble interface

Energy Technology Data Exchange (ETDEWEB)

Takahira, Hiroyuki, E-mail: takahira@me.osakafu-u.ac.jp; Ogasawara, Toshiyuki, E-mail: oga@me.osakafu-u.ac.jp; Mori, Naoto, E-mail: su101064@edu.osakafu-u.ac.jp; Tanaka, Moe [Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai-shi, Osaka 599-8531 (Japan)

2015-10-28

The secondary cavitation that occurs by the backscattering of focused ultrasound from a primary cavitation bubble caused by the negative pressure part of the ultrasound (Maxwell, et al., 2011) might be useful for the energy exchange due to bubble oscillations in High Intensity Focused Ultrasound (HIFU). The present study is concerned with the cavitation inception by the backscattering of ultrasound from a bubble. In the present experiment, a laser-induced bubble which is generated by a pulsed focused laser beam with high intensity is utilized as a primary cavitation bubble. After generating the bubble, focused ultrasound is emitted to the bubble. The acoustic field and the bubble motion are observed with a high-speed video camera. It is confirmed that the secondary cavitation bubble clouds are generated by the backscattering from the laser-induced bubble. The growth of cavitation bubble clouds is analyzed with the image processing method. The experimental results show that the height and width of the bubble clouds grow in stepwise during their evolution. The direct numerical simulations are also conducted for the backscattering of incident pressure waves from a bubble in order to evaluate a pressure field near the bubble. It is shown that the ratio of a bubble collapse time t{sub 0} to a characteristic time of wave propagation t{sub S}, η = t{sub 0}/t{sub s}, is an important determinant for generating negative pressure region by backscattering. The minimum pressure location by the backscattering in simulations is in good agreement with the experiment.

Remote sensing of particle dynamics: a two-component unmixing model in a western UK shelf sea.

Science.gov (United States)

Mitchell, Catherine; Cunningham, Alex

2014-05-01

The relationship between the backscattering and absorption coefficients, in particular the backscattering to absorption ratio, is mediated by the type of particles present in the water column. By considering the optical signals to be driven by phytoplankton and suspended minerals, with a relatively constant influence from CDOM, radiative transfer modelling is used to propose a method for retrieving the optical contribution of phytoplankton and suspended minerals to the total absorption coefficient with mean percentage errors of below 5% for both components. These contributions can be converted to constituent concentrations if the appropriate specific inherent optical properties are known or can be determined from the maximum and minimum backscattering to absorption ratios of the data. Remotely sensed absorption and backscattering coefficients from eight years of MODIS data for the Irish Sea reveal maximum backscattering to absorption coefficient ratios over the winter (with an average for the region of 0.27), which then decrease to a minimum over the summer months (with an average of 0.06) before increasing again through to winter, indicating a change in the particles present in the water column. Application of the two-component unmixing model to this data showed seasonal cycles of both phytoplankton and suspended mineral concentrations which vary in both amplitude and periodicity depending on their location. For example, in the Bristol Channel the amplitude of the suspended mineral concentration throughout one cycle is approximately 75% greater than a yearly cycle in the eastern Irish Sea. These seasonal cycles give an insight into the complex dynamics of particles in the water column, indicating the suspension of sediment throughout the winter months and the loss of sediments from the surface layer over the summer during stratification. The relationship between the timing of the phytoplankton spring bloom and changes in the availability of light in the water

Comparative determination of phenytoin by spectrophotometry, gas chromatography, liquid chromatography, enzyme immunoassay, and radioimmunoassay

International Nuclear Information System (INIS)

Castro, A.; Ibanez, J.; DiCesare, J.L.; Adams, R.F.; Malkus, H.

1978-01-01

Sera from patients being treated with phenytoin were analyzed for the drug by spectrophotometry, gas chromatography, radioimmunoasay, enzyme immunoassay, and liquid chromatography. The assay values obtained were intercompared statistically. Enzyme immunoassay and liquid chromatography appear to be attractive alternatives to the more traditional methods of spectrophotometry and gas chromatography. Our radioimmunoassay data correlated poorly with results by the four other methods

Compton backscattered collmated X-ray source

Science.gov (United States)

Ruth, Ronald D.; Huang, Zhirong

2000-01-01

A high-intensity, inexpensive and collimated x-ray source for applications such as x-ray lithography is disclosed. An intense pulse from a high power laser, stored in a high-finesse resonator, repetitively collides nearly head-on with and Compton backscatters off a bunched electron beam, having relatively low energy and circulating in a compact storage ring. Both the laser and the electron beams are tightly focused and matched at the interaction region inside the optical resonator. The laser-electron interaction not only gives rise to x-rays at the desired wavelength, but also cools and stabilizes the electrons against intrabeam scattering and Coulomb repulsion with each other in the storage ring. This cooling provides a compact, intense bunch of electrons suitable for many applications. In particular, a sufficient amount of x-rays can be generated by this device to make it an excellent and flexible Compton backscattered x-ray (CBX) source for high throughput x-ray lithography and many other applications.

Compton backscattered collimated x-ray source

Science.gov (United States)

Ruth, R.D.; Huang, Z.

1998-10-20

A high-intensity, inexpensive and collimated x-ray source is disclosed for applications such as x-ray lithography is disclosed. An intense pulse from a high power laser, stored in a high-finesse resonator, repetitively collides nearly head-on with and Compton backscatters off a bunched electron beam, having relatively low energy and circulating in a compact storage ring. Both the laser and the electron beams are tightly focused and matched at the interaction region inside the optical resonator. The laser-electron interaction not only gives rise to x-rays at the desired wavelength, but also cools and stabilizes the electrons against intrabeam scattering and Coulomb repulsion with each other in the storage ring. This cooling provides a compact, intense bunch of electrons suitable for many applications. In particular, a sufficient amount of x-rays can be generated by this device to make it an excellent and flexible Compton backscattered x-ray (CBX) source for high throughput x-ray lithography and many other applications. 4 figs.

A facility for low energy charged particle induced reaction studies

International Nuclear Information System (INIS)

Vilaithong, T.; Singkarat, S.; Yu, L.D.; Intarasiri, S.; Tippawan, U.

2000-01-01

In Chiang Mai, a highly stable low energy ion accelerator (0 - 350 kV) facility is being established. A subnano-second pulsing system will be incorporated into the beam transport line. The detecting system will consist of a time-of-flight charged particle spectrometer and a high resolution gamma-ray system. The new facility will be used in the studies of low energy heavy ion backscattering and charged particle induced cross section measurement in the interests of material characterization and nucleosynthesis. (author)

Tropospheric aerosol backscatter background mode at CO2 wavelengths

Science.gov (United States)

Rothermel, Jeffry; Bowdle, David A.; Menzies, Robert T.; Post, Madison J.; Vaughan, J. Michael

1989-01-01

A comparison is made between three climatologies of backscatter measurements in the troposphere and lower stratosphere at CO2 wavelengths. These were obtained from several locations using ground-based and airborne lidar systems. All three measurement sets show similar features, specifically, a high frequency of occurrence of low backscatter over a limited range of values in the middle and upper atmosphere (the 'background mode'). This background mode is important for the design and performance simulation of the prospective satellite sensors that rely on atmospheric aerosols as scattering targets.

Morning sector drift-bounce resonance driven ULF waves observed in artificially-induced HF radar backscatter

Directory of Open Access Journals (Sweden)

L. J. Baddeley

Full Text Available HF radar backscatter, which has been artificially-induced by a high power RF facility such as the EISCAT heater at Tromsø, has provided coherent radar ionospheric electric field data of unprecedented temporal resolution and accuracy. Here such data are used to investigate ULF wave processes observed by both the CUTLASS HF radars and the EISCAT UHF radar. Data from the SP-UK-OUCH experiment have revealed small-scale (high azimuthal wave number, m -45 waves, predominantly in the morning sector, thought to be brought about by the drift-bounce resonance processes. Conjugate observations from the Polar CAM-MICE instrument indicate the presence of a non-Maxwellian ion distribution function. Further statistical analysis has been undertaken, using the Polar TIMAS instrument, to reveal the prevalence and magnitude of the non-Maxwellian energetic particle populations thought to be responsible for generating these wave types.

Key words. Ionosphere (active experiments; wave-particle interactions Magnetospheric physics (MHD waves and instabilities

Morning sector drift-bounce resonance driven ULF waves observed in artificially-induced HF radar backscatter

Directory of Open Access Journals (Sweden)

L. J. Baddeley

2002-09-01

Full Text Available HF radar backscatter, which has been artificially-induced by a high power RF facility such as the EISCAT heater at Tromsø, has provided coherent radar ionospheric electric field data of unprecedented temporal resolution and accuracy. Here such data are used to investigate ULF wave processes observed by both the CUTLASS HF radars and the EISCAT UHF radar. Data from the SP-UK-OUCH experiment have revealed small-scale (high azimuthal wave number, m -45 waves, predominantly in the morning sector, thought to be brought about by the drift-bounce resonance processes. Conjugate observations from the Polar CAM-MICE instrument indicate the presence of a non-Maxwellian ion distribution function. Further statistical analysis has been undertaken, using the Polar TIMAS instrument, to reveal the prevalence and magnitude of the non-Maxwellian energetic particle populations thought to be responsible for generating these wave types.Key words. Ionosphere (active experiments; wave-particle interactions Magnetospheric physics (MHD waves and instabilities

Stimulated Brillouin backscattering losses in weakly inhomogeneous laser-produced plasmas

International Nuclear Information System (INIS)

Eidmann, K.; Brederlow, G.; Brodmann, R.; Petsch, R.; Sigel, R.; Tsarkiris, G.; Volk, R.; Witkowski, S.

1979-02-01

Studies of the reflection from a plane solid target plasma produced with a 1TW iodine laser (lambda = 1.3μm) at pulse durations of 300 ps are presented. The specularly reflected and the backscattered light was observed separately at different angles of incidence, intensities and spot sizes (up to 400 μm). Stimulated Brillouin scattering was identified as the main mechanism for backscattering with saturation at 20 - 30% reflection. (orig.) [de

Application of the Ultraviolet Scanning Elastic Backscatter LiDAR for the Investigation of Aerosol Variability

Directory of Open Access Journals (Sweden)

Fei Gao

2015-05-01

Full Text Available In order to investigate the aerosol variability over the southwest region of Slovenia, an ultraviolet scanning elastic backscatter LiDAR was utilized to make the vertical scan for atmospheric probing. With the assumption of horizontal atmospheric homogeneity, aerosol optical variables were retrieved from the horizontal pixel data points of two-dimensional range-height-indicator (RHI diagrams by using a multiangle retrieval method, in which optical depth is defined as the slope of the resulting linear function when height is kept constant. To make the data retrieval feasible and precise, a series of key procedures complemented the data processing, including construction of the RHI diagram, correction of Rayleigh scattering, assessment of horizontal atmospheric homogeneity and retrieval of aerosol optical variables. The measurement example demonstrated the feasibility of the ultraviolet scanning elastic backscatter LiDAR in the applications of the retrieval of aerosol extinction and determination of the atmospheric boundary layer height. Three months’ data combined with the modeling of air flow trajectories using Hybrid Single Particle Lagrangian Integrated Trajectory Model were analyzed to investigate aerosol variability. The average value of aerosol extinction with the presence of land-based air masses from the European continent was found to be two-times larger than that influenced by marine aerosols from the Mediterranean or Adriatic Sea.

Studies on direct and indirect electrochemical immunoassays

OpenAIRE

Buckley, Eileen

1989-01-01

Two approaches to electrochemical immunoassay are reported. The first approach was an indirect method, involving an electroactive, enzyme-catalysed, substrate to product reaction. Conditions were optimised for the amperometric detection of para-aminophenol, the electroactive product of the alkaline phosphatase catalysed hydrolysis of a new substrate, p-aminophenylphosphate, after separation by HPLC. The second approach involved the direct electrochemical detection of an immunoglo...

A compact Compton backscatter X-ray source for mammography and coronary angiography

International Nuclear Information System (INIS)

Nguyen, D.C.; Kinross-Wright, J.M.; Weber, M.E.; Volz, S.K.; Gierman, S.M.; Hayes, K.; Vernon, W.; Goldstein, D.J.

1998-01-01

This is the final report of a three-year, Laboratory Directed Research and Development (LDRD) project at Los Alamos National Laboratory (LANL). The project objective is to generate a large flux of tunable, monochromatic x-rays for use in mammography and coronary angiography. The approach is based on Compton backscattering of an ultraviolet solid-state laser beam against the high-brightness 20-MeV electron beams from a compact linear accelerator. The direct Compton backscatter approach failed to produce a large flux of x-rays due to the low photon flux of the scattering solid-state laser. The authors have modified the design of a compact x-ray source to the new Compton backscattering geometry with use of a regenerative amplifier free-electron laser. They have successfully demonstrated the production of a large flux of infrared photons and a high-brightness electron beam focused in both dimensions for performing Compton backscattering in a regenerative amplifier geometry

High Resolution Bathymetry and Backscatter of a High-Frequency Test Area

National Research Council Canada - National Science Library

Flood, Roger

2001-01-01

.... This is due to the presence of east-west trending wave-generated ripples with amplitudes of about 15 cm and wavelengths of about 75 cm on the ridge, with backscatter being higher when the sound beams are perpendicular to the ripples. The backscatter patterns on multi beam records may thus be used for determining the presence and orientation of small-scale topographic relief.

The Correlation Characteristics of Polarization Backscattering Matrix of Dense Chaff Clouds

Directory of Open Access Journals (Sweden)

B. Tang

2018-04-01

Full Text Available This paper studied the correlation characteristics of the polarization backscattering matrix of the dense chaff cloud with uniform orientation and location distributions in circular symmetry region. Based on the theoretical analysis and numerical experiments, the correlation coefficients of the four elements in the polarization backscattering matrix are obtained, and the results indicate that the cross to co-polar correlation coefficient is still zero; and that the sum of the co-polar cross-correlation coefficient and the two times of linear depolarization ratio equals one. The results are beneficial for better understanding of the backscattering characteristics of dense chaff clouds, and are useful in the application of jamming recognition in radar electronic warfare. Numerical experiments are performed by using the method of moments.

Test particle modeling of wave-induced energetic electron precipitation

International Nuclear Information System (INIS)

Chang, H.C.; Inan, U.S.

1985-01-01

A test particle computer model of the precipitation of radiation belt electrons is extended to compute the dynamic energy spectrum of transient electron fluxes induced by short-duration VLF wave packets traveling along the geomagnetic field lines. The model is adapted to estimate the count rate and associated spectrum of precipitated electrons that would be observed by satellite-based particle detectors with given geometric factor and orientation with respect to the magnetic field. A constant-frequency wave pulse and a lightning-induced whistler wave packet are used as examples of the stimulating wave signals. The effects of asymmetry of particle mirror heights in the two hemispheres and the atmospheric backscatter of loss cone particles on the computed precipitated fluxes are discussed

Which amphetamine-type stimulants can be detected by oral fluid immunoassays?

Science.gov (United States)

Souza, Daniele Z; Boehl, Paula O; Comiran, Eloisa; Prusch, Débora S; Zancanaro, Ivomar; Fuentefria, Alexandre M; Pechansky, Flavio; Duarte, Paulina C A V; De Boni, Raquel B; Fröehlich, Pedro E; Limberger, Renata P

2012-02-01

The use of oral fluid for monitoring drug consumption on roads has many advantages over conventional biological fluids; therefore, several immunoassays have been developed for this purpose. In this work, the ability of 3 commercial immunoassays to detect amphetamine-type stimulants (ATSs) in oral fluid was assessed. In addition, it was reviewed the main controlled ATSs available worldwide, as well as the oral fluid immunological screening tests that have been used for identifying ATSs in drivers. The analytical specificity of amphetamine direct enzyme-linked immunosorbent assay (ELISA), methamphetamine direct ELISA (Immunalysis Corporation), and Oral-View saliva multidrug of abuse test (Alfa Scientific Designs) was evaluated using ATS-spiked oral fluid. Legislation and published articles that report the use of immunological screening tests to detect ATS consumption in conductors were reviewed, including the kit's technical information, project reports, police and drug databases. Even at high concentrations, the tested assays were not able to detect methylphenidate, fenproporex, or diethylpropion, controlled ATSs legally marketed in many countries. This evidences the need to develop new kits that enable one to control the misuse of prescription ATSs on roads through oral fluid immunoassays.

Plasmon enhanced fluoro-immunoassay using egg yolk antibodies for ultra-sensitive detection of herbicide diuron.

Science.gov (United States)

Sharma, Priyanka; Kukkar, Manil; Ganguli, Ashok K; Bhasin, Aman; Suri, C Raman

2013-08-07

Plasmon enhanced fluorescence immunoassay (PEFI) format has been reported in developing a sensitive heterogeneous fluoroimmunoassay for monitoring the phenylurea herbicide diuron. Computer-assisted molecular modeling was carried out to study the conformational and electrostatic effects of synthesized hapten for producing highly specific egg yolk antibody against a phenyl urea herbicide diuron. The generated antibodies were labeled with fluorescein isothiocyanate at different molar ratios and used as tracer in the developed fluorescence based immunoassay. The sensitivity of the assay format was enhanced by using silver nanoparticles tagged with bovine serum albumin as a new blocking reagent in the developed PEFI format. Enhancer treatment on the developed immunoassay showed a significant improvement of fluorescence signal intensity with approximately 10 fold increase in assay sensitivity. The immunoassay has a detection limit of 0.01 ng mL(-1) with good signal precision (~2%) in the optimum working concentration range between 1 pg mL(-1) to 10 μg mL(-1) of diuron. These findings facilitate high throughput fluorescence-based processes that could be useful in biology, drug discovery and compound screening applications.

Radio-analysis of hydrogenous material using neutron back-scattering technique

International Nuclear Information System (INIS)

Holly, Wiam Ahmed Alteghany

2014-10-01

In this work, we have explored the possibility of using neutron back-scattering technique in performing radio analysis for samples of hydrogenous materials such as explosives, drugs, crude oil and water, looking for different signals that may be used to discriminate these samples. Monte Carlo simulations were carried out to model the detection system and select the optimal geometry as well. The results were determined in terms of the energy spectra of the back-scattered neutrons.(Author)

RFID tag modification for full depth backscatter modulation

Science.gov (United States)

Scott, Jeffrey Wayne [Pasco, WA; Pratt, Richard M [Richland, WA

2010-07-20

A modulated backscatter radio frequency identification device includes a diode detector configured to selectively modulate a reply signal onto an incoming continuous wave; communications circuitry configured to provide a modulation control signal to the diode detector, the diode detector being configured to modulate the reply signal in response to be modulation control signal; and circuitry configured to increase impedance change at the diode detector which would otherwise not occur because the diode detector rectifies the incoming continuous wave while modulating the reply signal, whereby reducing the rectified signal increases modulation depth by removing the reverse bias effects on impedance changes. Methods of improving depth of modulation in a modulated backscatter radio frequency identification device are also provided.

Standard-target calibration of an acoustic backscatter system

Science.gov (United States)

Foote, Kenneth G.; Martini, Marinna A.

2010-01-01

The standard-target method used to calibrate scientific echo sounders and other scientific sonars by a single, solid elastic sphere is being adapted to acoustic backscatter (ABS) systems. Its first application, to the AQUAscat 1000, is described. The on-axis sensitivity and directional properties of transducer beams at three operating frequencies, nominally 1, 2.5, and 4 MHz, have been determined using a 10-mm-diameter sphere of tungsten carbide with 6% cobalt binder. Preliminary results are reported for the 1-MHz transducer. Their application to measurements of suspended sediment made in situ with the same device is described. This will enable the data to be expressed directly in physical units of volume backscattering.

ISIS 1 measurements of high-frequency backscatter inside the ionosphere

International Nuclear Information System (INIS)

James, H.G.

1989-01-01

A study has been made of high-frequency backscatter observed by the ISIS 1 topside sounder at orbital heights from approximately 600 to 800 km. Signals are detected during high-latitude passes at radar ranges x of up to about 400 km and at frequencies from about the X mode cutoff at the spacecraft (usually around 5 MHz) up to 10 MHz typically and 18 MHz occasionally. The signals are interpreted as coherent aspect-sensitive scatter. The absolute power of return signals at high latitudes usually varies as x -2 for x ≤ 150 km. This implies that the scattering cross-section density σ is constant for all azimuths about the magnetic field and for ranges out to 150 km or, in other words, that irregularity patches have a constant σ over cross-field dimensions of 300 km. At larger ranges the signal often falls off more sharply with x, indicating azimuthal variations in σ. The cross section scaled from the data using the radar equation is found to have values centered near 10 -8 to 10 -7 m -1 for the most intense signals, referred to an assumed polar-aspect angle sensitivity. However, the magnitude of σ drops by about 2 orders of magnitude in the frequency range 5-15 MHz. Previous statistical studies have established that strong backscatter is restricted to auroral latitudes. Here the polar distribution of backscatter has been plotted for two sets of passes over the south pole, one collected during austral winter and the other during summer. The strongest backscatter is located at invariant latitudes around 80 degree. Backscatter is significantly stronger and distributed more extensively over the polar cap in winter than in summer

Forty-five degree backscattering-mode nonlinear absorption imaging in turbid media.

Science.gov (United States)

Cui, Liping; Knox, Wayne H

2010-01-01

Two-color nonlinear absorption imaging has been previously demonstrated with endogenous contrast of hemoglobin and melanin in turbid media using transmission-mode detection and a dual-laser technology approach. For clinical applications, it would be generally preferable to use backscattering mode detection and a simpler single-laser technology. We demonstrate that imaging in backscattering mode in turbid media using nonlinear absorption can be obtained with as little as 1-mW average power per beam with a single laser source. Images have been achieved with a detector receiving backscattered light at a 45-deg angle relative to the incoming beams' direction. We obtain images of capillary tube phantoms with resolution as high as 20 microm and penetration depth up to 0.9 mm for a 300-microm tube at SNR approximately 1 in calibrated scattering solutions. Simulation results of the backscattering and detection process using nonimaging optics are demonstrated. A Monte Carlo-based method shows that the nonlinear signal drops exponentially as the depth increases, which agrees well with our experimental results. Simulation also shows that with our current detection method, only 2% of the signal is typically collected with a 5-mm-radius detector.

Fake news? Biotin interference in thyroid immunoassays.

Science.gov (United States)

Koehler, Viktoria F; Mann, Ulrike; Nassour, Ayham; Alexander Mann, W

2018-05-29

We report on a 47 year old male patient with multiple sclerosis (MS) presenting in our outpatient neurology clinic in Frankfurt/Main for therapy evaluation. Before change of treatment laboratory investigations were performed. Thyroid function tests (TFTs) with a streptavidin/biotin based immunoassay revealed severe hyperthyroidism with positive thyroid autoantibodies suggestive for Graves' disease. Clinical presentation and thyroid sonography were unremarkable. Due to the discordance between clinical presentation and TFTs, we repeated medical history, in which the patient reported taking high-doses of biotin (300 mg/day) for MS. Recent studies with patients suffering from primary and secondary progressive MS, indicated promising effects of high-dose biotin on MS-related disability. In immunoassays relaying on streptavidin-biotin interaction, biotin intake can cause falsely high or low results. Two weeks after withdrawing biotin, biotin/streptavidin dependant assays showed no longer the biochemical picture of severe hyperthyroidism. Biotin intake should be paused for at least two to five days prior to the use of biotin/streptavidin dependant assays. Alternatively, non-biotin/streptavidin dependant assays (radioimmunoassay, gas chromatography-mass spectrometry/liquid chromatography-mass spectrometry) may be used. Copyright © 2017. Published by Elsevier B.V.

Theory of CW lidar aerosol backscatter measurements and development of a 2.1 microns solid-state pulsed laser radar for aerosol backscatter profiling

Science.gov (United States)

Kavaya, Michael J.; Henderson, Sammy W.; Frehlich, R. G.

1991-01-01

The performance and calibration of a focused, continuous wave, coherent detection CO2 lidar operated for the measurement of atmospheric backscatter coefficient, B(m), was examined. This instrument functions by transmitting infrared (10 micron) light into the atmosphere and collecting the light which is scattered in the rearward direction. Two distinct modes of operation were considered. In volume mode, the scattered light energy from many aerosols is detected simultaneously, whereas in the single particle mode (SPM), the scattered light energy from a single aerosol is detected. The analysis considered possible sources of error for each of these two cases, and also considered the conditions where each technique would have superior performance. The analysis showed that, within reasonable assumptions, the value of B(m) could be accurately measured by either the VM or the SPM method. The understanding of the theory developed during the analysis was also applied to a pulsed CO2 lidar. Preliminary results of field testing of a solid state 2 micron lidar using a CW oscillator is included.

Analysis of seafloor backscatter strength dependence on the survey azimuth using multibeam echosounder data

Science.gov (United States)

Lurton, Xavier; Eleftherakis, Dimitrios; Augustin, Jean-Marie

2018-06-01

The sediment backscatter strength measured by multibeam echosounders is a key feature for seafloor mapping either qualitative (image mosaics) or quantitative (extraction of classifying features). An important phenomenon, often underestimated, is the dependence of the backscatter level on the azimuth angle imposed by the survey line directions: strong level differences at varying azimuth can be observed in case of organized roughness of the seabed, usually caused by tide currents over sandy sediments. This paper presents a number of experimental results obtained from shallow-water cruises using a 300-kHz multibeam echosounder and specially dedicated to the study of this azimuthal effect, with a specific configuration of the survey strategy involving a systematic coverage of reference areas following "compass rose" patterns. The results show for some areas a very strong dependence of the backscatter level, up to about 10-dB differences at intermediate oblique angles, although the presence of these ripples cannot be observed directly—neither from the bathymetry data nor from the sonar image, due to the insufficient resolution capability of the sonar. An elementary modeling of backscattering from rippled interfaces explains and comforts these observations. The consequences of this backscatter dependence upon survey azimuth on the current strategies of backscatter data acquisition and exploitation are discussed.

Optical Modeling of Spectral Backscattering and Remote Sensing Reflectance From Emiliania huxleyi Blooms

Directory of Open Access Journals (Sweden)

Griet Neukermans

2018-05-01

Full Text Available In this study we develop an analytical model for spectral backscattering and ocean color remote sensing of blooms of the calcifying phytoplankton species Emiliania huxleyi. Blooms of this coccolithophore species are ubiquitous and particularly intense in temperate and subpolar ocean waters. We first present significant improvements to our previous analytical light backscattering model for E. huxleyi coccoliths and coccospheres by accounting for the elliptical shape of coccoliths and the multi-layered coccosphere architecture observed on detailed imagery of E. huxleyi liths and coccospheres. Our new model also includes a size distribution function that closely matches measured E. huxleyi size distributions. The model for spectral backscattering is then implemented in an analytical radiative transfer model to evaluate the variability of spectral remote sensing reflectance with respect to changes in the size distribution of the coccoliths and during a hypothetical E. huxleyi bloom decay event in which coccospheres shed their liths. Our modeled remote sensing reflectance spectra reproduced well the bright milky turquoise coloring of the open ocean typically associated with the final stages of E. huxleyi blooms, with peak reflectance at a wavelength of 0.49 μm. Our results also show that the magnitude of backscattering from coccoliths when attached to or freed from the coccosphere does not differ much, contrary to what is commonly assumed, and that the spectral shape of backscattering is mainly controlled by the size and morphology of the coccoliths, suggesting that they may be estimated from spectral backscattering.

Ion backscattering techniques applied in materials science research

International Nuclear Information System (INIS)

Sood, D.K.

1978-01-01

The applications of Ion Backscattering Technique (IBT) to material analysis have expanded rapidly during the last decade. It is now regarded as an analysis tool indispensable for a versatile materials research program. The technique consists of simply shooting a beam of monoenergetic ions (usually 4 He + ions at about 2 MeV) onto a target, and measuring their energy distribution after backscattering at a fixed angle. Simple Rutherford scattering analysis of the backscattered ion spectrum yields information on the mass, the absolute amount and the depth profile of elements present upto a few microns of the target surface. The technique is nondestructive, quick, quantitative and the only known method of analysis which gives quantitative results without recourse to calibration standards. Its major limitations are the inability to separate elements of similar mass and a complete absence of chemical-binding information. A typical experimental set up and spectrum analysis have been described. Examples, some of them based on the work at the Bhabha Atomic Research Centre, Bombay, have been given to illustrate the applications of this technique to semiconductor technology, thin film materials science and nuclear energy materials. Limitations of IBT have been illustrated and a few remedies to partly overcome these limitations are presented. (auth.)

Comparison of SeaWinds Backscatter Imaging Algorithms

Science.gov (United States)

Long, David G.

2017-01-01

This paper compares the performance and tradeoffs of various backscatter imaging algorithms for the SeaWinds scatterometer when multiple passes over a target are available. Reconstruction methods are compared with conventional gridding algorithms. In particular, the performance and tradeoffs in conventional ‘drop in the bucket’ (DIB) gridding at the intrinsic sensor resolution are compared to high-spatial-resolution imaging algorithms such as fine-resolution DIB and the scatterometer image reconstruction (SIR) that generate enhanced-resolution backscatter images. Various options for each algorithm are explored, including considering both linear and dB computation. The effects of sampling density and reconstruction quality versus time are explored. Both simulated and actual data results are considered. The results demonstrate the effectiveness of high-resolution reconstruction using SIR as well as its limitations and the limitations of DIB and fDIB. PMID:28828143

Evaluation of two automated chemiluminescence immunoassays, the LIAISON Treponema Screen and the ARCHITECT Syphilis TP, and the Treponema pallidum particle agglutination test for laboratory diagnosis of syphilis.

Science.gov (United States)

Wellinghausen, Nele; Dietenberger, Hanna

2011-08-01

Automated Treponema pallidum-specific chemi-luminescence immunoassays (CLIA) run on random-access analyzers allow for rapid diagnosis of syphilis infection. We evaluated the LIAISON Treponema Screen (LIA) and the ARCHITECT Syphilis TP (ARCH) in comparison to the Treponema pallidum particle agglutination (TPPA) test, as a screening test for syphilis. We performed a prospective study using 577 sera submitted for diagnosis of syphilis, including 318 samples from pregnant women. In addition, 42 stored sera from 32 patients with clinically and serologically characterized syphilis infection were investigated. In the prospective study, the sensitivity and specificity of LIA, ARCH, and TPPA were 100% (18/18), 100% (17/17), and 100% (18/18), and 100% (558/558), 99.8% (552/553), and 99.6% (556/558), respectively. In pregnant women, the specificity of LIA and ARCH was 100% (317/317) and of TPPA 99.7% (316/317). One sample from a child with assumed exposure to maternal antitreponemal antibodies was omitted from analysis. LIA, ARCH, and TPPA were also positive in all investigated sera from patients with known syphilis. Both automated CLIA demonstrated excellent diagnostic sensitivity and specificity when evaluated as a screening test for syphilis under routine conditions of a diagnostic laboratory. Thus, these may be used independently as an alternative to the manual TPPA screen.

Diffuse optical microscopy for quantification of depth-dependent epithelial backscattering in the cervix

Science.gov (United States)

Bodenschatz, Nico; Lam, Sylvia; Carraro, Anita; Korbelik, Jagoda; Miller, Dianne M.; McAlpine, Jessica N.; Lee, Marette; Kienle, Alwin; MacAulay, Calum

2016-06-01

A fiber optic imaging approach is presented using structured illumination for quantification of almost pure epithelial backscattering. We employ multiple spatially modulated projection patterns and camera-based reflectance capture to image depth-dependent epithelial scattering. The potential diagnostic value of our approach is investigated on cervical ex vivo tissue specimens. Our study indicates a strong backscattering increase in the upper part of the cervical epithelium caused by dysplastic microstructural changes. Quantization of relative depth-dependent backscattering is confirmed as a potentially useful diagnostic feature for detection of precancerous lesions in cervical squamous epithelium.

Electron backscattering for process control in electron beam welding

International Nuclear Information System (INIS)

Ardenne, T. von; Panzer, S.

1983-01-01

A number of solutions to the automation of electron beam welding is presented. On the basis of electron backscattering a complex system of process control has been developed. It allows an enlarged imaging of the material's surface, improved adjustment of the beam focusing and definite focus positioning. Furthermore, both manual and automated positioning of the electron beam before and during the welding process has become possible. Monitoring of the welding process for meeting standard welding requirements can be achieved with the aid of a control quantity derived from the results of electronic evaluation of the high-frequency electron backscattering

Application of the particle backscattering methods for the study of new oxide protective coatings at the surface of Al and Mg alloys

International Nuclear Information System (INIS)

Apelfeld, A.V.; Bespalova, O.V.; Borisov, A.M.; Dunkin, O.N.; Goryaga, N.G.; Kulikauskas, V.S.; Romanovsky, E.A.; Semenov, S.V.; Souminov, I.V.

2000-01-01

Rutherford (1.5 MeV 4 He + ) and Nuclear (7.7 MeV H + ) Backscattering Spectrometry were used for investigation of oxide protective coatings on the surface of Al and Mg alloys obtained by microarc oxidation (MAO). A model of microarc coating formation is proposed. For Mg alloy, the structure of MAO coating with very high corrosion resistance was determined

Simple patterned nanofiber scaffolds and its enhanced performance in immunoassay.

Directory of Open Access Journals (Sweden)

Jing Wang

Full Text Available Cancer has become the leading cause of death worldwide; early diagnosis and treatment of cancers is critical for the survival of the patients. The concentration of cancer markers in easy-to-access biological fluids can provide great assistance in screening for occult primary cancers, distinguishing malignant from benign findings, determining prognosis and prediction for cancer patients. The multiplex detection technology of a panel of cancer markers can greatly increase the accuracy of disease diagnosis. Herein, we briefly fabricate a high-throughput micro-immunoassay based on the electrospun polystyrene (PS substrates to improve detection sensitivity. The immunoassay was evaluated by analyzing three different cancer biomarkers (AFP, CEA, VEGF. For AFP, CEA, VEGF immunofluorescence assay, the LOD of assay conducted on electrospun PS substrates before or after plasma and the conventional PS substrates were 0.42, 0.10, 1.12 ng/mL, 0.57, 0.09, 1.24 ng/mL, and 159.75, 26.19, 385.59 pg/mL, respectively (P < 0.05. Due to the high porosity and large surface area-to-volume ratio which is the foremost merit of nanostructures, and the plasma treatment which make the hydrophobic PS nanofibers hydropholic, the nanofibers substrates showed sufficient retention of immunoassay functionality and high potential for capture molecules immobilization. Consequently, the immunofluorescence assay conducted on electrospun PS substrates could significantly enhance the sensitivity and limits of detection.

Deoxynivalenol-mimic nanobody isolated from a naïve phage display nanobody library and its application in immunoassay.

Science.gov (United States)

Qiu, Yu-Lou; He, Qing-Hua; Xu, Yang; Bhunia, Arun K; Tu, Zhui; Chen, Bo; Liu, Yuan-Yuan

2015-08-05

In this study, using mycotoxin deoxynivalenol (DON) as a model hapten, we developed a nanobody-based environmental friendly immunoassay for sensitive detection of DON. Two nanobodies (N-28 and N-31) which bind to anti-DON monoclonal antibody (MAb) were isolated from a naive phage display library. These nanobodies are clonable, thermally stable and mycotoxin-free products and can be served as coating antigen mimetics in heterologous immunoassay. The half inhibition concentration (IC50) of the immunoassay developed with N-28 and N-31 was 8.77 ± 0.41 ng mL(-1) and 19.97 ± 0.84 ng mL(-1), respectively, which were 18- and 8-fold more sensitive than the conventional coating antigen (DON-BSA) based immunoassay. In order to better understand the molecular mechanism of antigen mimicry by nanobody, the 3D structure of "nanobody (N-28) - anti-DON MAb" complex was presented and verified by molecular modeling and alanine-scanning mutagenesis. The results showed that hydrogen bond and hydrophobic interaction formed between Thr 102 - Ser 106 of N-28 and CDR H3 residues of anti-DON antibody may contribute to their binding. This novel concept of enhancing sensitivity of immunoassay for DON based on nanobody may provide potential applications in a general method for immunoassay of various food chemical contaminants. Copyright © 2015 Elsevier B.V. All rights reserved.

Balanced and optimal bianisotropic particles: maximizing power extracted from electromagnetic fields

International Nuclear Information System (INIS)

Ra'di, Younes; Tretyakov, Sergei A

2013-01-01

Here we introduce the concept of ‘optimal particles’ for strong interactions with electromagnetic fields. We assume that a particle occupies a given electrically small volume in space and study the required optimal relations between the particle polarizabilities. In these optimal particles, the inclusion shape and material are chosen so that the particles extract the maximum possible power from given incident fields. It appears that for different excitation scenarios the optimal particles are bianisotropic chiral, omega, moving and Tellegen particles. The optimal dimensions of resonant canonical chiral and omega particles are found analytically. Such optimal particles have extreme properties in scattering (e.g., zero backscattering or invisibility). Planar arrays of optimal particles possess extreme properties in reflection and transmission (e.g. total absorption or magnetic-wall response), and volumetric composites of optimal particles realize, for example, such extreme materials as the chiral nihility medium. (paper)

Development of a Nanobody-Based Lateral Flow Immunoassay for Detection of Human Norovirus.

Science.gov (United States)

Doerflinger, Sylvie Y; Tabatabai, Julia; Schnitzler, Paul; Farah, Carlo; Rameil, Steffen; Sander, Peter; Koromyslova, Anna; Hansman, Grant S

2016-01-01

Human noroviruses are the dominant cause of outbreaks of acute gastroenteritis. These viruses are usually detected by molecular methods, including reverse transcriptase PCR (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). Human noroviruses are genetically and antigenically diverse, with two main genogroups that are further subdivided into over 40 different genotypes. During the past decade, genogroup 2 genotype 4 (GII.4) has dominated in most countries, but recently, viruses belonging to GII.17 have increased in prevalence in a number of countries. A number of commercially available ELISAs and lateral flow immunoassays were found to have lower sensitivities to the GII.17 viruses, indicating that the antibodies used in these methods may not have a high level of cross-reactivity. In this study, we developed a rapid Nanobody-based lateral flow immunoassay (Nano-immunochromatography [Nano-IC]) for the detection of human norovirus in clinical specimens. The Nano-IC assay detected virions from two GII.4 norovirus clusters, which included the current dominant strain and a novel variant strain. The Nano-IC method had a sensitivity of 80% and specificity of 86% for outbreak specimens. Norovirus virus-like particles (VLPs) representing four genotypes (GII.4, GII.10, GII.12, and GII.17) could be detected by this method, demonstrating the potential in clinical screening. However, further modifications to the Nano-IC method are needed in order to improve this sensitivity, which may be achieved by the addition of other broadly reactive Nanobodies to the system. IMPORTANCE We previously identified a Nanobody (termed Nano-85) that bound to a highly conserved region on the norovirus capsid. In this study, the Nanobody was biotinylated and gold conjugated for a lateral flow immunoassay (termed Nano-IC). We showed that the Nano-IC assay was capable of detecting at least four antigenically distinct GII genotypes, including the newly emerging GII.17. In the clinical setting, the

Optimized Lateral Flow Immunoassay Reader for the Detection of Infectious Diseases in Developing Countries.

Science.gov (United States)

Pilavaki, Evdokia; Demosthenous, Andreas

2017-11-20

Detection and control of infectious diseases is a major problem, especially in developing countries. Lateral flow immunoassays can be used with great success for the detection of infectious diseases. However, for the quantification of their results an electronic reader is required. This paper presents an optimized handheld electronic reader for developing countries. It features a potentially low-cost, low-power, battery-operated device with no added optical accessories. The operation of this proof of concept device is based on measuring the reflected light from the lateral flow immunoassay and translating it into the concentration of the specific analyte of interest. Characterization of the surface of the lateral flow immunoassay has been performed in order to accurately model its response to the incident light. Ray trace simulations have been performed to optimize the system and achieve maximum sensitivity by placing all the components in optimum positions. A microcontroller enables all the signal processing to be performed on the device and a Bluetooth module allows transmission of the results wirelessly to a mobile phone app. Its performance has been validated using lateral flow immunoassays with influenza A nucleoprotein in the concentration range of 0.5 ng/mL to 200 ng/mL.

Optimized Lateral Flow Immunoassay Reader for the Detection of Infectious Diseases in Developing Countries

Directory of Open Access Journals (Sweden)

Evdokia Pilavaki

2017-11-01

Full Text Available Detection and control of infectious diseases is a major problem, especially in developing countries. Lateral flow immunoassays can be used with great success for the detection of infectious diseases. However, for the quantification of their results an electronic reader is required. This paper presents an optimized handheld electronic reader for developing countries. It features a potentially low-cost, low-power, battery-operated device with no added optical accessories. The operation of this proof of concept device is based on measuring the reflected light from the lateral flow immunoassay and translating it into the concentration of the specific analyte of interest. Characterization of the surface of the lateral flow immunoassay has been performed in order to accurately model its response to the incident light. Ray trace simulations have been performed to optimize the system and achieve maximum sensitivity by placing all the components in optimum positions. A microcontroller enables all the signal processing to be performed on the device and a Bluetooth module allows transmission of the results wirelessly to a mobile phone app. Its performance has been validated using lateral flow immunoassays with influenza A nucleoprotein in the concentration range of 0.5 ng/mL to 200 ng/mL.

Recommendations for processing atmospheric attenuated backscatter profiles from Vaisala CL31 ceilometers

Science.gov (United States)

Kotthaus, Simone; O'Connor, Ewan; Münkel, Christoph; Charlton-Perez, Cristina; Haeffelin, Martial; Gabey, Andrew M.; Grimmond, C. Sue B.

2016-08-01

Ceilometer lidars are used for cloud base height detection, to probe aerosol layers in the atmosphere (e.g. detection of elevated layers of Saharan dust or volcanic ash), and to examine boundary layer dynamics. Sensor optics and acquisition algorithms can strongly influence the observed attenuated backscatter profiles; therefore, physical interpretation of the profiles requires careful application of corrections. This study addresses the widely deployed Vaisala CL31 ceilometer. Attenuated backscatter profiles are studied to evaluate the impact of both the hardware generation and firmware version. In response to this work and discussion within the CL31/TOPROF user community (TOPROF, European COST Action aiming to harmonise ground-based remote sensing networks across Europe), Vaisala released new firmware (versions 1.72 and 2.03) for the CL31 sensors. These firmware versions are tested against previous versions, showing that several artificial features introduced by the data processing have been removed. Hence, it is recommended to use this recent firmware for analysing attenuated backscatter profiles. To allow for consistent processing of historic data, correction procedures have been developed that account for artefacts detected in data collected with older firmware. Furthermore, a procedure is proposed to determine and account for the instrument-related background signal from electronic and optical components. This is necessary for using attenuated backscatter observations from any CL31 ceilometer. Recommendations are made for the processing of attenuated backscatter observed with Vaisala CL31 sensors, including the estimation of noise which is not provided in the standard CL31 output. After taking these aspects into account, attenuated backscatter profiles from Vaisala CL31 ceilometers are considered capable of providing valuable information for a range of applications including atmospheric boundary layer studies, detection of elevated aerosol layers, and model

Influence of Plastic Deformation of Steel Samples on the Fast electron Backscattering

International Nuclear Information System (INIS)

Sierra Trujillo, J. X.; Herrera Palma, V.; Desdin Garcia, L. F.; Codorniu Pujals, D.

2013-01-01

A considerable fraction of a fast electron beam incident on a target is scattered in backward direction. It is a very complex process involving electron - nucleus and electron - electron collisions. The fraction of backscattered electrons is described by a parameterization as a function of the atomic number and energy of the incident electrons. In such approaches the possible influence of the material structure is not taken into account. In this paper, the behavior of the 90 Sr/ 90 Y backscattered electrons from 08JuA and 15GJuT steel strained samples is investigated. A clear dependence between the degree of plastic deformation and the fraction of backscattered electrons was observed. This relationship is explained by the interaction of electrons with the dislocations in the material, whose density depends on the magnitude of the strain in the plastic region. On the basis of a simple model for describing this interaction, a mathematical expression is obtained for the relationship between the fraction of backscattered electrons and the degree of deformation. (Author)

Modification of Spalart-Allmaras model with consideration of turbulence energy backscatter using velocity helicity

International Nuclear Information System (INIS)

Liu, Yangwei; Lu, Lipeng; Fang, Le; Gao, Feng

2011-01-01

The correlation between the velocity helicity and the energy backscatter is proved in a DNS case of 256 3 -grid homogeneous isotropic decaying turbulence. The helicity is then proposed to be employed to improve turbulence models and SGS models. Then Spalart-Allmaras turbulence model (SA) is modified with the helicity to take account of the energy backscatter, which is significant in the region of corner separation in compressors. By comparing the numerical results with experiments, it can be concluded that the modification for SA model with helicity can appropriately represent the energy backscatter, and greatly improves the predictive accuracy for simulating the corner separation flow in compressors. -- Highlights: → We study the relativity between the velocity helicity and the energy backscatter. → Spalart-Allmaras turbulence model is modified with the velocity helicity. → The modified model is employed to simulate corner separation in compressor cascade. → The modification can greatly improve the accuracy for predicting corner separation. → The helicity can represent the energy backscatter in turbulence and SGS models.

IMMUNOASSAYS FOR THE DETECTION OF UROKINASE RECEPTOR FORMS

DEFF Research Database (Denmark)

2004-01-01

amounts of uPAR forms in a sample with data indicative of the presence of the cancer disease. The method can be used for staging, prognosis or diagnosis of prostate cancer. Two novel monoclonal antibodies and a kit and immunoassays for detecting at least one uPAR form(s) are also described in the present...

Direct salivary cortisol radio-immunoassay determination. Clinical applications

International Nuclear Information System (INIS)

Simon, C.; Cherfan, J.; Kurtz, F.; Vignon, F.; Schlienger, J.L.; Chabrier, G.

1987-01-01

Salivary cortisol levels reflect the biologically active free fraction of blood cortisol. The authors describe the results obtained with the aim of a radio-immunoassay commercial serum cortisol kit, without prealable extraction in different physiological and pathological situations. Salivary cortisol determination appears performant both in nycthemeral studies and in stimulation or freination tests [fr

The Full Aperture Backscatter Station Measurement System on the National Ignition Facility

International Nuclear Information System (INIS)

Bower, D; McCarville, T; Alvarez, S; Ault, L; Brown, M; Chrisp, M; Damian, C; DeHope, W; Froula, D; Glenzer, S; Grace, S; Gu, K; Holdener, F; Huffer, C; Kamperschroer, J; Kelleher, T; Kimbrough, J

2004-01-01

A Full Aperture Backscatter Station (FABS) target diagnostic has been activated on the first four beams of the National Ignition Facility (NIF). Backscattered light from the target propagates back down the beam path into the FABS diagnostic system. FABS measures both stimulated Brillouin scattering (SBS) and stimulated Raman scattering (SRS) with a suite of measurement instruments. Digital cameras and spectrometers record spectrally resolved energy for both P and S polarized light. Streaked spectrometers measure the spectral and temporal behavior of the backscattered light. Calorimeters and fast photodetectors measure the integrated energy and temporal behavior of the light, respectively. This paper provides an overview of the FABS measurements system and detailed descriptions of the diagnostic instruments and the optical path

Evaluation of backscatter dose from internal lead shielding in clinical electron beams using EGSnrc Monte Carlo simulations.

Science.gov (United States)

De Vries, Rowen J; Marsh, Steven

2015-11-08

Internal lead shielding is utilized during superficial electron beam treatments of the head and neck, such as lip carcinoma. Methods for predicting backscattered dose include the use of empirical equations or performing physical measurements. The accuracy of these empirical equations required verification for the local electron beams. In this study, a Monte Carlo model of a Siemens Artiste linac was developed for 6, 9, 12, and 15 MeV electron beams using the EGSnrc MC package. The model was verified against physical measurements to an accuracy of better than 2% and 2mm. Multiple MC simulations of lead interfaces at different depths, corresponding to mean electron energies in the range of 0.2-14 MeV at the interfaces, were performed to calculate electron backscatter values. The simulated electron backscatter was compared with current empirical equations to ascertain their accuracy. The major finding was that the current set of backscatter equations does not accurately predict electron backscatter, particularly in the lower energies region. A new equation was derived which enables estimation of electron backscatter factor at any depth upstream from the interface for the local treatment machines. The derived equation agreed to within 1.5% of the MC simulated electron backscatter at the lead interface and upstream positions. Verification of the equation was performed by comparing to measurements of the electron backscatter factor using Gafchromic EBT2 film. These results show a mean value of 0.997 ± 0.022 to 1σ of the predicted values of electron backscatter. The new empirical equation presented can accurately estimate electron backscatter factor from lead shielding in the range of 0.2 to 14 MeV for the local linacs.

Waterfall notch-filtering for restoration of acoustic backscatter records from Admiralty Bay, Antarctica

Science.gov (United States)

Fonseca, Luciano; Hung, Edson Mintsu; Neto, Arthur Ayres; Magrani, Fábio José Guedes

2018-06-01

A series of multibeam sonar surveys were conducted from 2009 to 2013 around Admiralty Bay, Shetland Islands, Antarctica. These surveys provided a detailed bathymetric model that helped understand and characterize the bottom geology of this remote area. Unfortunately, the acoustic backscatter records registered during these bathymetric surveys were heavily contaminated with noise and motion artifacts. These artifacts persisted in the backscatter records despite the fact that the proper acquisition geometry and the necessary offsets and delays were applied during the survey and in post-processing. These noisy backscatter records were very difficult to interpret and to correlate with gravity-core samples acquired in the same area. In order to address this issue, a directional notch-filter was applied to the backscatter waterfall in the along-track direction. The proposed filter provided better estimates for the backscatter strength of each sample by considerably reducing residual motion artifacts. The restoration of individual samples was possible since the waterfall frame of reference preserves the acquisition geometry. Then, a remote seafloor characterization procedure based on an acoustic model inversion was applied to the restored backscatter samples, generating remote estimates of acoustic impedance. These remote estimates were compared to Multi Sensor Core Logger measurements of acoustic impedance obtained from gravity core samples. The remote estimates and the Core Logger measurements of acoustic impedance were comparable when the shallow seafloor was homogeneous. The proposed waterfall notch-filtering approach can be applied to any sonar record, provided that we know the system ping-rate and sampling frequency.

Modeling variability in dendritic ice crystal backscattering cross sections at millimeter wavelengths using a modified Rayleigh–Gans theory

International Nuclear Information System (INIS)

Lu, Yinghui; Clothiaux, Eugene E.; Aydin, Kültegin; Botta, Giovanni; Verlinde, Johannes

2013-01-01

Using the Generalized Multi-particle Mie-method (GMM), Botta et al. (in this issue) [7] created a database of backscattering cross sections for 412 different ice crystal dendrites at X-, Ka- and W-band wavelengths for different incident angles. The Rayleigh–Gans theory, which accounts for interference effects but ignores interactions between different parts of an ice crystal, explains much, but not all, of the variability in the database of backscattering cross sections. Differences between it and the GMM range from −3.5 dB to +2.5 dB and are highly dependent on the incident angle. To explain the residual variability a physically intuitive iterative method was developed to estimate the internal electric field within an ice crystal that accounts for interactions between the neighboring regions within it. After modifying the Rayleigh–Gans theory using this estimated internal electric field, the difference between the estimated backscattering cross sections and those from the GMM method decreased to within 0.5 dB for most of the ice crystals. The largest percentage differences occur when the form factor from the Rayleigh–Gans theory is close to zero. Both interference effects and neighbor interactions are sensitive to the morphology of ice crystals. Improvements in ice-microphysical models are necessary to predict or diagnose internal structures within ice crystals to aid in more accurate interpretation of radar returns. Observations of the morphology of ice crystals are, in turn, necessary to guide the development of such ice-microphysical models and to better understand the statistical properties of ice crystal morphologies in different environmental conditions. -- Highlights: • Significant variability exists in radar backscattering cross sections of dendrites. • Source of variability depends upon detailed distribution of mass within dendrites. • The Rayleigh–Gans theory (RG) captures most of the variability. • Improving RG by estimating dendrite

Microfluidic "Pouch" Chips for Immunoassays and Nucleic Acid Amplification Tests.

Science.gov (United States)

Mauk, Michael G; Liu, Changchun; Qiu, Xianbo; Chen, Dafeng; Song, Jinzhao; Bau, Haim H

2017-01-01

Microfluidic cassettes ("chips") for processing and analysis of clinical specimens and other sample types facilitate point-of-care (POC) immunoassays and nucleic acid based amplification tests. These single-use test chips can be self-contained and made amenable to autonomous operation-reducing or eliminating supporting instrumentation-by incorporating laminated, pliable "pouch" and membrane structures for fluid storage, pumping, mixing, and flow control. Materials and methods for integrating flexible pouch compartments and diaphragm valves into hard plastic (e.g., acrylic and polycarbonate) microfluidic "chips" for reagent storage, fluid actuation, and flow control are described. We review several versions of these pouch chips for immunoassay and nucleic acid amplification tests, and describe related fabrication techniques. These protocols thus offer a "toolbox" of methods for storage, pumping, and flow control functions in microfluidic devices.

A sandwich immunoassay for human prolyl 4-hydroxylase using monoclonal antibody

International Nuclear Information System (INIS)

Yoshida, Shinichi

1986-01-01

Monoclonal antibody was used in a sandwich enzyme immunoassay and in a radioimmunoassay for human serum immunoreactive prolyl 4-hydroxylase. The enzyme immunoassay utilized a monoclonal antibody as a solid phase and horseradish peroxidase-labeled rabbit antibody to human prolyl 4-hydroxylase as a conjugate. Sensitivity was 0.1 ng of enzyme per tube. With a conjugate purified by an enzyme-bound affinity column, sensitivity was increased to 0.01 ng per tube, and linearity was obtained between 0.01 to 30 ng per tube. The radioimmunoassay used a 125 I-labeled rabbit antibody (IgG) as the conjugate. Sensitivity of this technique was 0.4 ng of enzyme per tube. (Auth.)

Multi-angle backscatter classification and sub-bottom profiling for improved seafloor characterization

Science.gov (United States)

Alevizos, Evangelos; Snellen, Mirjam; Simons, Dick; Siemes, Kerstin; Greinert, Jens

2018-06-01

This study applies three classification methods exploiting the angular dependence of acoustic seafloor backscatter along with high resolution sub-bottom profiling for seafloor sediment characterization in the Eckernförde Bay, Baltic Sea Germany. This area is well suited for acoustic backscatter studies due to its shallowness, its smooth bathymetry and the presence of a wide range of sediment types. Backscatter data were acquired using a Seabeam1180 (180 kHz) multibeam echosounder and sub-bottom profiler data were recorded using a SES-2000 parametric sonar transmitting 6 and 12 kHz. The high density of seafloor soundings allowed extracting backscatter layers for five beam angles over a large part of the surveyed area. A Bayesian probability method was employed for sediment classification based on the backscatter variability at a single incidence angle, whereas Maximum Likelihood Classification (MLC) and Principal Components Analysis (PCA) were applied to the multi-angle layers. The Bayesian approach was used for identifying the optimum number of acoustic classes because cluster validation is carried out prior to class assignment and class outputs are ordinal categorical values. The method is based on the principle that backscatter values from a single incidence angle express a normal distribution for a particular sediment type. The resulting Bayesian classes were well correlated to median grain sizes and the percentage of coarse material. The MLC method uses angular response information from five layers of training areas extracted from the Bayesian classification map. The subsequent PCA analysis is based on the transformation of these five layers into two principal components that comprise most of the data variability. These principal components were clustered in five classes after running an external cluster validation test. In general both methods MLC and PCA, separated the various sediment types effectively, showing good agreement (kappa >0.7) with the Bayesian

Retrieval method of aerosol extinction coefficient profile based on backscattering, side-scattering and Raman-scattering lidar

Science.gov (United States)

Shan, Huihui; Zhang, Hui; Liu, Junjian; Tao, Zongming; Wang, Shenhao; Ma, Xiaomin; Zhou, Pucheng; Yao, Ling; Liu, Dong; Xie, Chenbo; Wang, Yingjian

2018-03-01

Aerosol extinction coefficient profile is an essential parameter for atmospheric radiation model. It is difficult to get higher signal to noise ratio (SNR) of backscattering lidar from the ground to the tropopause especially in near range. Higher SNR problem can be solved by combining side-scattering and backscattering lidar. Using Raman-scattering lidar, aerosol extinction to backscatter ratio (lidar ratio) can be got. Based on side-scattering, backscattering and Raman-scattering lidar system, aerosol extinction coefficient is retrieved precisely from the earth's surface to the tropopause. Case studies show this method is reasonable and feasible.

Significance of isolated reactive treponemal chemiluminescence immunoassay results.

Science.gov (United States)

Hunter, Michael G; Robertson, Peter W; Post, Jeffrey J

2013-05-01

Isolated reactive serum treponemal chemiluminescence immunoassay (CIA) specimens cause clinical uncertainty. Sera were screened by CIA, and reactive samples underwent reflex testing with rapid plasma reagin (RPR), Treponema pallidum particle agglutination (TPPA), and fluorescent treponemal antibody absorption (FTA Abs) assays. Samples reactive only on the CIA were deemed "isolated" reactive CIA samples. We undertook detailed review of a subset of subjects with isolated reactive CIA specimens. Of 28 261 specimens, 1171 (4.1%) were reactive on CIA, of which 133 (11.3%) had isolated CIA reactivity. Most subjects (66 of 82 [80.5%]) with isolated reactive CIA specimens were from high-prevalence populations. We found evidence of CIA, TPPA, and FTA Abs seroreversion. The median chemiluminescent signal-to-cutoff ratio was similar for isolated reactive CIA sera and sera that were reactive on either FTA Abs or TPPA assays (2.19 vs 2.32; P = .15) but lower than for sera reactive on both FTA Abs and TPPA assays (12.37; P < .001) or for sera reactive on RPR assays (25.53; P < .001). A total of 11 of 20 patients (55%) with an isolated reactive CIA specimen who underwent medical record review had previous or subsequent evidence of syphilis infection. Isolated reactive CIA specimens may represent true T. pallidum infection and may be found after seroreversion of traditional treponemal assays.

Immunoassay of β-endorphin

International Nuclear Information System (INIS)

Hoellt, V.; Gramsch, C.; Herz, A.

1979-01-01

The present paper describes the characteristics of a series of antisera against β-endorphin (β-E) developed in our laboratory which all recognize β-lipotropin and their use in (1) the determination of β-E in tissue and in body fluids and in (2) the immunocytochemical localization of β-E containing neurons in the rat brain and in (3) the study of the conversion of the β-E/β-LPH precursor into β-LPH and β-E in the pars intermedia/nervosa of the rat pituitary. It is the purpose of the present report to critically analyze the pitfalls and drawbacks, as well as the advantage, of the use of radioimmunoassay and other immunoassays in the determination of β-E. (Auth.)

A multislice theory of electron scattering in crystals including backscattering and inelastic effects.

Science.gov (United States)

Spiegelberg, Jakob; Rusz, Ján

2015-12-01

In the framework of the slice transition operator technique, a general multislice theory for electron scattering in crystals is developed. To achieve this generalization, we combine the approaches for inelastic scattering derived by Yoshioka [J. Phys. Soc. Jpn. 12, 6 (1957)] and backscattering based on the formalism of Chen and Van Dyck [Ultramicroscopy 70, 29-44 (1997)]. A computational realization of the obtained equations is suggested. The proposed computational scheme is tested on elastic backscattering of electrons, where we consider single backscattering in analogy to the computational scheme proposed by Chen and Van Dyck. Copyright © 2015 Elsevier B.V. All rights reserved.

Numerical simulation of range and backscattering for keV protons incident on random targets

International Nuclear Information System (INIS)

Robinson, J.E.; Agamy, S.

1975-01-01

Using a Monte-Carlo technique, projected range distributions and backscattering coefficients have been calculated for keV protons normally incident on heavy targets. For an incident reduced energy range of 1 less than epsilon 0 less than 20, both the projected range distributions and backscattering coefficients have been found to be in good agreement with a third order Edgeworth range approximation. Backscattered energy and angular distributions have also been calculated and are compared to available theoretical and experimental data. (4 figs.) (U.S.)

Towards the development of a radioenzyme-immunoassay

International Nuclear Information System (INIS)

Schuurs, A.H.W.M.; Waart, M. v. d.

1976-01-01

We have tried to develop a very sensitive enzyme-immunoassay. For this purpose, a very sensitive radiochemical enzyme assay was used. HCG was chosen as test model and AChE as labelling enzyme. The test appeared to be much more sensitive than the normal enzymeimmunoassay. And, in comparison with RIA, it was about as sensitive but less time-consuming, and it makes use, in principle, of stable reagents. (orig./GSE) [de

Modification of a deoxynivalenol-antigen-mimicking nanobody to improve immunoassay sensitivity by site-saturation mutagenesis.

Science.gov (United States)

Qiu, Yu-Lou; He, Qing-Hua; Xu, Yang; Wang, Wei; Liu, Yuan-Yuan

2016-01-01

A nanobody (N-28) which can act as a deoxynivalenol (DON) antigen has been generated, and its residues Thr102-Ser106 were identified to bind with anti-DON monoclonal antibody by alanine-scanning mutagenesis. Site-saturation mutagenesis was used to analyze the plasticity of five residues and to improve the sensitivity of the N-28-based immunoassay. After mutagenesis, three mutants were selected by phage immunoassay and were sequenced. The half-maximal inhibitory concentrations of the immunoassay based on mutants N-28-T102Y, N-28-V103L, and N-28-Y105F were 24.49 ± 1.0, 51.83 ± 2.5, and 35.65 ± 1.6 ng/mL, respectively, showing the assay was, respectively, 3.2, 1.5, and 2.2 times more sensitive than the wild-type-based assay. The best mutant, N-28-T102Y, was used to develop a competitive phage ELISA to detect DON in cereals with high specificity and accuracy. In addition, the structural properties of N-28-T102Y and N-28 were investigated, revealing that the affinity of N-28-T102Y decreased because of increased steric hindrance with the large side chain. The lower-binding-affinity antigen mimetic may contribute to the improvement of the sensitivity of competitive immunoassays. These results demonstrate that nanobodies would be a favorable tool for engineering. Moreover, our results have laid a solid foundation for site-saturation mutagenesis of antigen-mimicking nanobodies to improve immunoassay sensitivity for small molecules.

Performance of hepatitis B assays on the Bayer ADVIA Centaur Immunoassay System.

Science.gov (United States)

van Helden, Josef; Denoyel, Gérard; Karwowska, Sylwia; Reamer, Randy; Schmalz, John; Wright, Ted; Preisel-Simmons, Barbara

2004-01-01

Bayer HealthCare LLC, Diagnostics Division, has developed several new assays on the ADVIA Centaur immunoassay system for the detection of markers of hepatitis B virus infection in human serum and plasma. This panel includes assays for: hepatitis B surface antigen (HBsAg), a confirmatory test method for HBsAg, antibodies to hepatitis B surface antigen (anti-HBs), IgM and IgG antibodies to hepatitis B core antigen (anti-HBc Total) and IgM antibodies to hepatitis B core antigen (anti-HBc IgM). These assays employ magnetic particle separation technology with direct chemiluminescence for optimal assay performance. All of the assays are fully automated, require sample volumes ranging from 15 microl to 100 microl (with the exception of the ADVIA Centaur HBsAg Confirmatory Assay, which requires 2 x 100 microl), and have throughputs of up to 240 tests per hour. The five ADVIA Centaur HBV assays were tested in extensive performance evaluations conducted at two sites in Europe. The performance evaluations, which included samples from HBV-infected individuals, blood donors, hospitalized/clinical patients, and HBV vaccinees (for Anti-HBs evaluation), generated performance data in support of obtaining the Communautés Européennes (CE) mark for European market distribution. The HBV performance evaluations resulted in an overall diagnostic specificity > 99%, i.e. 99.94% for the ADVIA Centaur HBsAg Assay, 100% for the ADVIA Centaur Anti-HBs Assay, 100% for the ADVIA Centaur HBc IgM Assay and 99.94% for the ADVIA Centaur HBc Total Assay. All of the ADVIA Centaur assays showed a very good diagnostic sensitivity on these populations with 100% for the ADVIA Centaur HBsAg Assay, 99.0% for the ADVIA Centaur Anti-HBs Assay, 98.53% for the ADVIA Centaur HBc IgM Assay and 100% for the ADVIA Centaur HBc Total Assay. The ADVIA Centaur HBsAg Confirmatory Test confirmed 100% of the positive HBsAg samples. Testing of interfering substances and potential cross-reacting samples for all ADVIA

Computation of Nonlinear Backscattering Using a High-Order Numerical Method

Science.gov (United States)

Fibich, G.; Ilan, B.; Tsynkov, S.

2001-01-01

The nonlinear Schrodinger equation (NLS) is the standard model for propagation of intense laser beams in Kerr media. The NLS is derived from the nonlinear Helmholtz equation (NLH) by employing the paraxial approximation and neglecting the backscattered waves. In this study we use a fourth-order finite-difference method supplemented by special two-way artificial boundary conditions (ABCs) to solve the NLH as a boundary value problem. Our numerical methodology allows for a direct comparison of the NLH and NLS models and for an accurate quantitative assessment of the backscattered signal.

Three-dimensional imaging of hidden objects using positron emission backscatter

International Nuclear Information System (INIS)

Lee, Dongwon; Cowee, Misa; Fenimore, Ed; Galassi, Mark; Looker, Quinn; Mcneil, Wendy V.; Stonehill, Laura; Wallace, Mark

2009-01-01

Positron emission backscatter imaging is a technique for interrogation and three-dimensional (3-D) reconstruction of hidden objects when we only have access to the objects from one side. Using time-of-flight differences in detected direct and backscattered positron-emitted photons, we construct 3-D images of target objects. Recently at Los Alamos National Laboratory, a fully three-dimensional imaging system has been built and the experimental results are discussed in this paper. Quantitative analysis of images reconstructed in both two- and three-dimensions are also presented.

Backscatter dose from metallic materials due to obliquely incident high-energy photon beams

International Nuclear Information System (INIS)

Nadrowitz, Roger; Feyerabend, Thomas

2001-01-01

If metallic material is exposed to ionizing radiation of sufficient high energy, an increase in dose due to backscatter radiation occurs in front of this material. Our purpose in this study was to quantify these doses at variable distances between scattering materials and the detector at axial beam angles between 0 deg. (zero angle in beams eye view) and 90 deg. . Copper, silver and lead sheets embedded in a phantom of perspex were exposed to 10 MV-bremsstrahlung. The detector we developed is based on the fluorescence property of pyromellitic acid (1,2,4,5 benzenetetracarboxylic acid) after exposure to ionizing radiation. Our results show that the additional doses and the corresponding dose distribution in front of the scattering materials depend quantitatively and qualitatively on the beam angle. The backscatter dose increases with varying beam angle from 0 deg. to 90 deg. up to a maximum at 55 deg. for copper and silver. At angles of 0 deg. and 55 deg. the integral backscatter doses over a tissue-equivalent depth of 2 mm are 11.2% and 21.6% for copper and 24% and 28% for silver, respectively. In contrast, in front of lead there are no obvious differences of the measured backscatter doses at angles between 0 deg. and 55 deg. With a further increase of the beam angle from 55 deg. to 90 deg. the backscatter dose decreases steeply for all three materials. In front of copper a markedly lower penetrating depth of the backscattered electrons was found for an angle of 0 deg. compared to 55 deg. This dependence from the beam angle was less pronounced in front of silver and not detectable in front of lead. In conclusion, the dependence of the backscatter dose from the angle between axial beam and scattering material must be considered, as higher scattering doses have to be considered than previously expected. This may have a clinical impact since the surface of metallic implants is usually curved

Backscatter dose from metallic materials due to obliquely incident high-energy photon beams

Energy Technology Data Exchange (ETDEWEB)

Nadrowitz, Roger; Feyerabend, Thomas [Medical University of Luebeck, Germany, Department of Radiotherapy and Nuclear Medicine, Ratzeburger Allee 160, Luebeck, D-23538 (Germany)

2001-06-01

If metallic material is exposed to ionizing radiation of sufficient high energy, an increase in dose due to backscatter radiation occurs in front of this material. Our purpose in this study was to quantify these doses at variable distances between scattering materials and the detector at axial beam angles between 0 deg. (zero angle in beams eye view) and 90 deg. . Copper, silver and lead sheets embedded in a phantom of perspex were exposed to 10 MV-bremsstrahlung. The detector we developed is based on the fluorescence property of pyromellitic acid (1,2,4,5 benzenetetracarboxylic acid) after exposure to ionizing radiation. Our results show that the additional doses and the corresponding dose distribution in front of the scattering materials depend quantitatively and qualitatively on the beam angle. The backscatter dose increases with varying beam angle from 0 deg. to 90 deg. up to a maximum at 55 deg. for copper and silver. At angles of 0 deg. and 55 deg. the integral backscatter doses over a tissue-equivalent depth of 2 mm are 11.2% and 21.6% for copper and 24% and 28% for silver, respectively. In contrast, in front of lead there are no obvious differences of the measured backscatter doses at angles between 0 deg. and 55 deg. With a further increase of the beam angle from 55 deg. to 90 deg. the backscatter dose decreases steeply for all three materials. In front of copper a markedly lower penetrating depth of the backscattered electrons was found for an angle of 0 deg. compared to 55 deg. This dependence from the beam angle was less pronounced in front of silver and not detectable in front of lead. In conclusion, the dependence of the backscatter dose from the angle between axial beam and scattering material must be considered, as higher scattering doses have to be considered than previously expected. This may have a clinical impact since the surface of metallic implants is usually curved.

Dual-Mode SERS-Fluorescence Immunoassay Using Graphene Quantum Dot Labeling on One-Dimensional Aligned Magnetoplasmonic Nanoparticles.

Science.gov (United States)

Zou, Fengming; Zhou, Hongjian; Tan, Tran Van; Kim, Jeonghyo; Koh, Kwangnak; Lee, Jaebeom

2015-06-10

A novel dual-mode immunoassay based on surface-enhanced Raman scattering (SERS) and fluorescence was designed using graphene quantum dot (GQD) labels to detect a tuberculosis (TB) antigen, CFP-10, via a newly developed sensing platform of linearly aligned magnetoplasmonic (MagPlas) nanoparticles (NPs). The GQDs were excellent bilabeling materials for simultaneous Raman scattering and photoluminescence (PL). The one-dimensional (1D) alignment of MagPlas NPs simplified the immunoassay process and enabled fast, enhanced signal transduction. With a sandwich-type immunoassay using dual-mode nanoprobes, both SERS signals and fluorescence images were recognized in a highly sensitive and selective manner with a detection limit of 0.0511 pg mL(-1).

Discordant Analytical Results Caused by Biotin Interference on Diagnostic Immunoassays in a Pediatric Hospital.

Science.gov (United States)

Ali, Mahesheema; Rajapakshe, Deepthi; Cao, Liyun; Devaraj, Sridevi

2017-09-01

Recent studies have reported that biotin interferes with certain immunoassays. In this study, we evaluated the analytical interference of biotin on immunoassays that use streptavidin-biotin in our pediatric hospital. We tested the effect of different concentrations of biotin (1.5-200 ng/ml) on TSH, Prolactin, Ferritin, CK-MB, β-hCG, Troponin I, LH, FSH, Cortisol, Anti-HAV antibody (IgG and IgM), assays on Ortho Clinical Diagnostic Vitros 5600 Analyzer. Biotin (up to 200 ng/mL) did not significantly affect Troponin I and HAV assays. Biotin (up to 12.5 ng/ml) resulted in biotin >6.25 ng/mL significantly affected TSH (>20% bias) assay. Prolactin was significantly affected even at low levels (Biotin 1.5 ng/mL). Thus, we recommend educating physicians about biotin interference in common immunoassays and adding an electronic disclaimer. © 2017 by the Association of Clinical Scientists, Inc.

340 nm pulsed UV LED system for europium-based time-resolved fluorescence detection of immunoassays

DEFF Research Database (Denmark)

Rodenko, Olga; Fodgaard, Henrik; Tidemand-Lichtenberg, Peter

2016-01-01

We report on the design, development and investigation of an optical system based on UV light emitting diode (LED) excitation at 340 nm for time-resolved fluorescence detection of immunoassays. The system was tested to measure cardiac marker Troponin I with a concentration of 200 ng....../L in immunoassay. The signal-to-noise ratio was comparable to state-of-the-art Xenon flash lamp based unit with equal excitation energy and without overdriving the LED. We performed a comparative study of the flash lamp and the LED based system and discussed temporal, spatial, and spectral features of the LED...... excitation for time-resolved fluorimetry. Optimization of the suggested key parameters of the LED promises significant increase of the signal-to-noise ratio and hence of the sensitivity of immunoassay systems....

340 nm pulsed UV LED system for europium-based time-resolved fluorescence detection of immunoassays.

Science.gov (United States)

Rodenko, Olga; Fodgaard, Henrik; Tidemand-Lichtenberg, Peter; Petersen, Paul Michael; Pedersen, Christian

2016-09-19

We report on the design, development and investigation of an optical system based on UV light emitting diode (LED) excitation at 340 nm for time-resolved fluorescence detection of immunoassays. The system was tested to measure cardiac marker Troponin I with a concentration of 200 ng/L in immunoassay. The signal-to-noise ratio was comparable to state-of-the-art Xenon flash lamp based unit with equal excitation energy and without overdriving the LED. We performed a comparative study of the flash lamp and the LED based system and discussed temporal, spatial, and spectral features of the LED excitation for time-resolved fluorimetry. Optimization of the suggested key parameters of the LED promises significant increase of the signal-to-noise ratio and hence of the sensitivity of immunoassay systems.

Implementation of a rapid HIT immunoassay at a university hospital - Retrospective analysis of HIT laboratory orders in patients with thrombocytopenia.

Science.gov (United States)

Black, Anne; Heimerl, Susanne; Oertli, Linnéa; Wilczek, Wolf; Greinacher, Andreas; Spannagl, Michael; Herr, Wolfgang; Hart, Christina

2017-10-01

Heparin-induced thrombocytopenia (HIT) is a rare cause of thrombocytopenia and a potentially life-threatening adverse drug reaction. Clinical overdiagnosis of HIT results in costly laboratory tests and anticoagulation. Criteria and algorithms for diagnosis are established, but their translation into clinical practice is still challenging. In a retrospective approach we studied all HIT related laboratory test requests within four years and evaluated data before (1st period, 24month) and after (2nd period, 24month) replacing particle gel immunoassay (PaGIA) and enzyme-linked immunosorbent assay (ELISA) by a chemiluminescent immunoassay (CLIA). HIT was confirmed by heparin-induced platelet activation (HIPA) test. Clinical pretest probability for HIT using an implemented simplified 4Ts score and platelet count were evaluated. Costs for laboratory tests and alternative anticoagulation were calculated. In 1850 patients with suspected HIT, 2327 laboratory orders were performed. In 87.2% of these orders an intermediate/high simplified 4Ts score was found. Thrombocytopenia was present in 87.1%. After replacing PaGIA and ELISA by CLIA the number of immunological and functional laboratory tests was reduced by 38.2%. The number of positive HIT immunoassays declined from 22.6% to 6.0%, while the number of positive HIPA tests among positive immunological tests increased by 19%. Altogether, acute HIT was confirmed in 59 patients. A decline in the use of alternative anticoagulants was observed in the 2nd period. Our study shows that in a university hospital setting HIT is well-known, but diagnosis requires a precise laboratory confirmation. Replacing PaGIA and ELISA by CLIA did not influence laboratory order behavior but results in reduced overall costs for laboratory diagnostics and alternative anticoagulation. Copyright © 2017 Elsevier Ltd. All rights reserved.

Moessbauer backscatter spectrometer with full data processing capability

International Nuclear Information System (INIS)

1976-01-01

The design and operation of a Moessbauer backscatter spectrometer with full data processing capability is described, and the investigation of the applicability of this technique to a variety of practical metallurgical problems is discussed

[Automated analyzer of enzyme immunoassay].

Science.gov (United States)

Osawa, S

1995-09-01

Automated analyzers for enzyme immunoassay can be classified by several points of view: the kind of labeled antibodies or enzymes, detection methods, the number of tests per unit time, analytical time and speed per run. In practice, it is important for us consider the several points such as detection limits, the number of tests per unit time, analytical range, and precision. Most of the automated analyzers on the market can randomly access and measure samples. I will describe the recent advance of automated analyzers reviewing their labeling antibodies and enzymes, the detection methods, the number of test per unit time and analytical time and speed per test.

Electrochemical immunoassay using magnetic beads for the determination of zearalenone in baby food: An anticipated analytical tool for food safety

International Nuclear Information System (INIS)

Hervas, Miriam; Lopez, Miguel Angel; Escarpa, Alberto

2009-01-01

In this work, electrochemical immunoassay involving magnetic beads to determine zearalenone in selected food samples has been developed. The immunoassay scheme has been based on a direct competitive immunoassay method in which antibody-coated magnetic beads were employed as the immobilisation support and horseradish peroxidase (HRP) was used as enzymatic label. Amperometric detection has been achieved through the addition of hydrogen peroxide substrate and hydroquinone as mediator. Analytical performance of the electrochemical immunoassay has been evaluated by analysis of maize certified reference material (CRM) and selected baby food samples. A detection limit (LOD) of 0.011 μg L -1 and EC 50 0.079 μg L -1 were obtained allowing the assessment of the detection of zearalenone mycotoxin. In addition, an excellent accuracy with a high recovery yield ranging between 95 and 108% has been obtained. The analytical features have shown the proposed electrochemical immunoassay to be a very powerful and timely screening tool for the food safety scene.

Electrochemical immunoassay using magnetic beads for the determination of zearalenone in baby food: an anticipated analytical tool for food safety.

Science.gov (United States)

Hervás, Miriam; López, Miguel Angel; Escarpa, Alberto

2009-10-27

In this work, electrochemical immunoassay involving magnetic beads to determine zearalenone in selected food samples has been developed. The immunoassay scheme has been based on a direct competitive immunoassay method in which antibody-coated magnetic beads were employed as the immobilisation support and horseradish peroxidase (HRP) was used as enzymatic label. Amperometric detection has been achieved through the addition of hydrogen peroxide substrate and hydroquinone as mediator. Analytical performance of the electrochemical immunoassay has been evaluated by analysis of maize certified reference material (CRM) and selected baby food samples. A detection limit (LOD) of 0.011 microg L(-1) and EC(50) 0.079 microg L(-1) were obtained allowing the assessment of the detection of zearalenone mycotoxin. In addition, an excellent accuracy with a high recovery yield ranging between 95 and 108% has been obtained. The analytical features have shown the proposed electrochemical immunoassay to be a very powerful and timely screening tool for the food safety scene.

Electrochemical immunoassay using magnetic beads for the determination of zearalenone in baby food: An anticipated analytical tool for food safety

Energy Technology Data Exchange (ETDEWEB)

Hervas, Miriam; Lopez, Miguel Angel [Departamento Quimica Analitica, Universidad de Alcala, Ctra. Madrid-Barcelona, Km. 33600, E-28871 Alcala de Henares, Madrid (Spain); Escarpa, Alberto, E-mail: alberto.escarpa@uah.es [Departamento Quimica Analitica, Universidad de Alcala, Ctra. Madrid-Barcelona, Km. 33600, E-28871 Alcala de Henares, Madrid (Spain)

2009-10-27

In this work, electrochemical immunoassay involving magnetic beads to determine zearalenone in selected food samples has been developed. The immunoassay scheme has been based on a direct competitive immunoassay method in which antibody-coated magnetic beads were employed as the immobilisation support and horseradish peroxidase (HRP) was used as enzymatic label. Amperometric detection has been achieved through the addition of hydrogen peroxide substrate and hydroquinone as mediator. Analytical performance of the electrochemical immunoassay has been evaluated by analysis of maize certified reference material (CRM) and selected baby food samples. A detection limit (LOD) of 0.011 {mu}g L{sup -1} and EC{sub 50} 0.079 {mu}g L{sup -1} were obtained allowing the assessment of the detection of zearalenone mycotoxin. In addition, an excellent accuracy with a high recovery yield ranging between 95 and 108% has been obtained. The analytical features have shown the proposed electrochemical immunoassay to be a very powerful and timely screening tool for the food safety scene.

Biosensor immunoassay for flumequine in broiler serum and muscle

NARCIS (Netherlands)

Haasnoot, W.; Gercek, H.; Cazemier, G.; Nielen, M.W.F.

2007-01-01

Flumequine (Flu) is one of the fluoroquinolones most frequently applied for the treatment of broilers in The Netherlands. For the detection of residues of Flu in blood serum of broilers, a biosensor immunoassay (BIA) was developed which was fast (7.5 min per sample) and specific (no cross-reactivity

A Strange Box and a Stubborn Brit: Rutherford's Experiments with Alpha Particles.

Science.gov (United States)

Digilov, M.

1991-01-01

Discusses 5 innovative experiments conducted by Rutherford in early 1900s utilizing the 30 milligrams of radium salt he personally carried from Europe to Canada in 1903. Traces his work with alpha particles from his original results which determined their nature, charge, and mass, to his technique of backscattering which helped to advance…

Measuring the stopping power of α particles in compact bone for BNCT

Science.gov (United States)

Provenzano, L.; Rodríguez, L. M.; Fregenal, D.; Bernardi, G.; Olivares, C.; Altieri, S.; Bortolussi, S.; González, S. J.

2015-01-01

The stopping power of α particles in thin films of decalcified sheep femur, in the range of 1.5 to 5.0 MeV incident energy, was measured by transmission of a backscattered beam from a heavy target. Additionally, the film elemental composition was determined by Rutherford Backscattering Spectrometry (RBS). These data will be used to measure boron concentration in thin films of bone using a spectrometry technique developed by the University of Pavia, since the concentration ratio between healthy tissue and tumor is of fundamental importance in Boron Neutron Capture Therapy (BNCT). The present experimental data are compared with numerical simulation results and with tabulated stopping power data of non-decalcified human bone.

Chimeric recombinant antibody fragments in cardiac troponin I immunoassay.

Science.gov (United States)

Hyytiä, Heidi; Heikkilä, Taina; Brockmann, Eeva-Christine; Kekki, Henna; Hedberg, Pirjo; Puolakanaho, Tarja; Lövgren, Timo; Pettersson, Kim

2015-03-01

To introduce a novel nanoparticle-based immunoassay for cardiac troponin I (cTnI) utilizing chimeric antibody fragments and to demonstrate that removal of antibody Fc-part and antibody chimerization decrease matrix related interferences. A sandwich-type immunoassay for cTnI based on recombinant chimeric (mouse variable/human constant) antigen binding (cFab) antibodies and intrinsically fluorescent nanoparticles was developed. To test whether using chimeric antibody fragments helps to avoid matrix related interferences, samples (n=39) with known amounts of triglycerides, bilirubin, rheumatoid factor (RF) or human anti-mouse antibodies (HAMAs) were measured with the novel assay, along with a previously published nanoparticle-based research assay with the same antibody epitopes. The limit of detection (LoD) was 3.30ng/L. Within-laboratory precision for 29ng/L and 2819ng/L cTnI were 13.7% and 15.9%, respectively. Regression analysis with Siemens ADVIA Centaur® yielded a slope (95% confidence intervals) of 0.18 (0.17-1.19) and a y-intercept of 1.94 (-1.28-3.91) ng/L. When compared to a previously published nanoparticle-based assay, the novel assay showed substantially reduced interference in the tested interference prone samples, 15.4 vs. 51.3%. A rheumatoid factor containing sample was decreased from 241ng/L to immunoassay for the detection of cTnI and decreased matrix related interferences, thus resulting in a lower number of falsely elevated cTnI-values. Copyright © 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

Forest anisotropy assessment by means of spatial variations analysis of PolSAR backscattering

Directory of Open Access Journals (Sweden)

A. V. Dmitriev

2017-06-01

Full Text Available The possibility to synthesize polarization response from earth covers at any desired combination of transmit and receive antenna polarizations is the significant advantage of polarimetric radar. It permits better identification of dominant scattering mechanisms especially when analyzing polarization signatures. These signatures depict more details of physical information from target backscattering in various polarization bases. However, polarization signatures cannot reveal spatial variations of the radar backscattering caused by volume heterogeneity of a target. This paper proposes a new approach for estimating volume target heterogeneity from polarimetric synthetic aperture radar (PolSAR images. The approach is based on the analysis of a novel type of polarization signature, which we call fractal polarization signature (FPS. This signature is a result of polarization synthesis of initial fully polarimetric data and subsequent fractal analysis of synthesized images. It is displayed as a 3D plot and can be produced for each point in an image. It is shown that FPS describes backscattering variations or image roughness at different states of polarization. Fully polarimetric data of SIR-C and ALOS PALSAR at ascending/descending orbits were used for testing the proposed approach. The azimuthal dependence of the radar backscattering variations is discovered when analyzing backscattering from a pine forest. It correlates with the results of a field survey of trees branch distribution.

Gliadin Detection in Food by Immunoassay

Science.gov (United States)

Grant, Gordon; Sporns, Peter; Hsieh, Y.-H. Peggy

Immunoassays are very sensitive and efficient tests that are commonly used to identify a specific protein. Examples of applications in the food industry include identification of proteins expressed in genetically modified foods, allergens, or proteins associated with a disease, including celiac disease. This genetic disease is associated with Europeans and affects about one in every 200 people in North America. These individuals react immunologically to wheat proteins, and consequently their own immune systems attack and damage their intestines. This disease can be managed if wheat proteins, specifically "gliadins," are avoided in foods.

Low-frequency acousto-optic backscattering of Bessel light beams

Science.gov (United States)

Khilo, Nikolai A.; Belyi, Vladimir N.; Khilo, Petr A.; Kazak, Nikolai S.

2018-05-01

The use of Bessel light beams, as well as Bessel acoustic beams, substantially enhances the capabilities of acousto-optic methods for control of optical field. We present a theoretical study of the process of optical Bessel beams conversion by means of backward acousto-optic scattering on a Bessel acoustic field in a transversely isotropic crystal. It is shown that, with an appropriate choice of Bessel beams parameters, the backscattering in visible spectral range can be realized at relatively low acoustic frequencies less than one gigahertz. Under conditions of phase matching and transverse spatial synchronism, the efficiency of backscattering is sufficiently high, which is interesting, for example, for construction of acousto-optic spectral analyzers.

Particle-in-cell plasma simulations of the modified two-stream instability

Directory of Open Access Journals (Sweden)

K. Schlegel

1994-08-01

Full Text Available We model the modified two-stream plasma instability occurring in the ionospheric E-region using a 2.5-dimensional particle-in-cell code. Compared to previous similar work we concentrate on simulated quantities that can easily be measured in the real ionosphere by coherent radars or rockets, such as the Doppler velocity, the backscattered power, backscattered spectra, aspect angle behaviour and electron temperature enhancement. Despite using a relatively small simulation model, we obtain remarkably good agreement between actual observed and simulated plasma parameters. The advantage of such a small system is that we were able to perform (other than in previous related work many simulation runs with different sets of input parameters, thus studying the unstable plasma under various conditions.

Numerical modelling of multiple scattering between two elastical particles

DEFF Research Database (Denmark)

Bjørnø, Irina; Jensen, Leif Bjørnø

1998-01-01

in suspension have been studied extensively since Foldy's formulation of his theory for isotropic scattering by randomly distributed scatterers. However, a number of important problems related to multiple scattering are still far from finding their solutions. A particular, but still unsolved, problem......Multiple acoustical signal interactions with sediment particles in the vicinity of the seabed may significantly change the course of sediment concentration profiles determined by inversion from acoustical backscattering measurements. The scattering properties of high concentrations of sediments...... is the question of proximity thresholds for influence of multiple scattering in terms of particle properties like volume fraction, average distance between particles or other related parameters. A few available experimental data indicate a significance of multiple scattering in suspensions where the concentration...

Particle stimulated nucleation during dynamic and metadynamic recrystallisation of Ni-30%Fe-Nb-C alloy

International Nuclear Information System (INIS)

Pereloma, Elena V.; Mannan, Parvez; Casillas, Gilberto; Saleh, Ahmed A.

2017-01-01

For the first time, a combination of scanning transmission electron microscopy and electron back-scattering diffraction is used to elucidate the early stages of particle stimulated recrystallisation at Nb carbides in Ni-30wt.%Fe alloy subjected to high temperature plane strain compression. While particles with sizes even below 1 μm were found to induce dynamic or metadynamic recrystallisation, only a small fraction of coarse particles served as nucleation sites. - Highlights: • The early stages of particle stimulated recrystallisation at Nb carbides are elucidated • A combination of transmission electron microscopy and electron back scattering diffraction used • Particles below 1 μm size could induce dynamic or metadynamic recrystallization

Particle stimulated nucleation during dynamic and metadynamic recrystallisation of Ni-30%Fe-Nb-C alloy

Energy Technology Data Exchange (ETDEWEB)

Pereloma, Elena V., E-mail: elenap@uow.edu.au [School of Mechanical, Materials and Mechatronics Engineering, University of Wollongong, Wollongong, NSW 2522 (Australia); UOW Electron Microscopy Centre, University of Wollongong, Wollongong, NSW 2500 (Australia); Mannan, Parvez [School of Mechanical, Materials and Mechatronics Engineering, University of Wollongong, Wollongong, NSW 2522 (Australia); Casillas, Gilberto [UOW Electron Microscopy Centre, University of Wollongong, Wollongong, NSW 2500 (Australia); Saleh, Ahmed A. [School of Mechanical, Materials and Mechatronics Engineering, University of Wollongong, Wollongong, NSW 2522 (Australia)

2017-03-15

For the first time, a combination of scanning transmission electron microscopy and electron back-scattering diffraction is used to elucidate the early stages of particle stimulated recrystallisation at Nb carbides in Ni-30wt.%Fe alloy subjected to high temperature plane strain compression. While particles with sizes even below 1 μm were found to induce dynamic or metadynamic recrystallisation, only a small fraction of coarse particles served as nucleation sites. - Highlights: • The early stages of particle stimulated recrystallisation at Nb carbides are elucidated • A combination of transmission electron microscopy and electron back scattering diffraction used • Particles below 1 μm size could induce dynamic or metadynamic recrystallization.

Detection of preferential particle orientation in the atmosphere: Development of an alternative polarization lidar system

International Nuclear Information System (INIS)

Geier, Manfred; Arienti, Marco

2014-01-01

Increasing interest in polarimetric characterization of atmospheric aerosols has led to the development of complete sample-measuring (Mueller) polarimeters that are capable of measuring the entire backscattering phase matrix of a probed volume. These Mueller polarimeters consist of several moving parts, which limit measurement rates and complicate data analysis. In this paper, we present the concept of a less complex polarization lidar setup for detection of preferential orientation of atmospheric particulates. On the basis of theoretical considerations of data inversion stability and propagation of measurement uncertainties, an optimum optical configuration is established for two modes of operation (with either a linear or a circular polarized incident laser beam). The conceptualized setup falls in the category of incomplete sample-measuring polarimeters and uses four detection channels for simultaneous measurement of the backscattered light. The expected performance characteristics are discussed through an example of a typical aerosol with a small fraction of particles oriented in a preferred direction. The theoretical analysis suggests that achievable accuracies in backscatter cross-sections and depolarization ratios are similar to those with conventional two-channel configurations, while in addition preferential orientation can be detected with the proposed four-channel system for a wide range of conditions. - Highlights: • A theoretical study of a new four-channel lidar concept is offered. • Preferential particle orientation detection could be realized with minor device modifications. • The proposed configuration is optimized to balance inversion uncertainties. • Circular polarized beam is demonstrated to provide the best noise performance. • Operation with ultra-short pulses is proposed to quantify particle number density

Bathymetry and acoustic backscatter-outer mainland shelf, eastern Santa Barbara Channel, California

Science.gov (United States)

Dartnell, Peter; Finlayson, David P.; Ritchie, Andrew C.; Cochrane, Guy R.; Erdey, Mercedes D.

2012-01-01

In 2010 and 2011, scientists from the U.S. Geological Survey (USGS), Pacific Coastal and Marine Science Center (PCMSC), acquired bathymetry and acoustic-backscatter data from the outer shelf region of the eastern Santa Barbara Channel, California. These surveys were conducted in cooperation with the Bureau of Ocean Energy Management (BOEM). BOEM is interested in maps of hard-bottom substrates, particularly natural outcrops that support reef communities in areas near oil and gas extraction activity. The surveys were conducted using the USGS R/V Parke Snavely, outfitted with an interferometric sidescan sonar for swath mapping and real-time kinematic navigation equipment. This report provides the bathymetry and backscatter data acquired during these surveys in several formats, a summary of the mapping mission, maps of bathymetry and backscatter, and Federal Geographic Data Committee (FGDC) metadata.

Response function of NaI(Tl) detectors and multiple backscattering of gamma rays in aluminium

International Nuclear Information System (INIS)

Sabharwal, Arvind D.; Singh, Manpreet; Singh, Bhajan; Sandhu, B.S.

2008-01-01

The response function, converting the observed pulse-height distribution of a NaI(Tl) detector to a true photon spectrum, is obtained experimentally with the help of an inverse matrix approach. The energy of gamma-ray photons continuously decreases as the number of scatterings increases in a sample having finite dimensions when one deals with the depth of the sample. The present experiments are undertaken to study the effect of target thickness on intensity distribution of gamma photons multiply backscattered from an aluminium target. A NaI(Tl) gamma-ray detector detects the photons backscattered from the aluminium target. The subtraction of analytically estimated singly scattered distribution from the observed intensity distribution (originating from interactions of primary gamma-ray photons with the target) results in multiply backscattered events. We observe that for each incident gamma photon energy, the number of multiply backscattered photons increases with increase in target thickness and then saturates at a particular target thickness called the saturation thickness (depth). Saturation thickness for multiply backscattering of gamma photons is found to decrease with increase in energy of incident gamma-ray photons

Jet-Tagged Back-Scattering Photons for Quark Gluon Plasma Tomography

Energy Technology Data Exchange (ETDEWEB)

Fries, Rainer J. [Cyclotron Institute and Department of Physics and Astronomy, Texas A and M University, College Station, TX 77845 (United States); De, Somnath; Srivastava, Dinesh K. [Variable Energy Cyclotron Centre, 1/AF, Bidhan Nagar, Kolkata – 700064 (India)

2013-05-02

Direct photons are important probes for quark gluon plasma created in high energy nuclear collisions. Various sources of direct photons in nuclear collisions are known, each of them endowed with characteristic information about the production process. However, it has been challenging to separate direct photon sources through measurements of single inclusive photon spectra and photon azimuthal asymmetry. Here we explore a method to identify photons created from the back-scattering of high momentum quarks off quark gluon plasma. We show that the correlation of back-scattering photons with a trigger jet leads to a signal that should be measurable at RHIC and LHC.

Anisotropy of the apparent frequency dependence of backscatter in formalin fixed human myocardium.

Science.gov (United States)

Hall, C S; Verdonk, E D; Wickline, S A; Perez, J E; Miller, J G

1997-01-01

Measurements of the frequency dependence of ultrasonic backscatter are presented for specific angles of insonification for regions of infarcted and noninfarcted human myocardium. A 5-MHz transducer was used to insonify cylindrical cores taken from 7 noninfarcted regions and 12 infarcted regions of the left ventricular free wall of 6 formalin-fixed human hearts explanted because of ischemic cardiomyopathy. The dependence of apparent (uncompensated for diffraction effects and attenuation) backscatter on frequency was approximated by a power-law dependence, magnitude of B(f)2 = afn. Under ideal conditions in a lossless medium, the effect of not compensating for the effects of diffraction and attenuation leads to the value of n to be 2.0 for Rayleigh scatterers while the frequency dependence of the fully compensated backscatter coefficient would be f4. The value of n was determined over the frequency range, 3-7 MHz. Both nonifarcted and infarcted myocardium exhibited anisotropy of the frequency dependence of backscatter, with maxima occurring at angles that were perpendicular to the predominant myofiber direction and minima when parallel to the fibers. Perpendicular insonification yielded results for n of 1.8 +/- 0.1 for noninfarcted myocardium and 1.2 +/- 0.1 for infarcted myocardium while parallel insonification yielded results of 0.4 +/- 0.1 for noninfarcted and 0.0 +/- 0.1 for infarcted myocardium. The functional form of the angle-dependent backscatter is similar for both noninfarcted and infarcted myocardium, although the frequency dependence is clearly different for both tissue states for all angles of insonification. The results of this study indicate that the anisotropy of the frequency dependence of backscatter may play a significant role in ultrasonic imaging and is an important consideration for ultrasonic tissue characterization in myocardium.

Recommendations for improved and coherent acquisition and processing of backscatter data from seafloor-mapping sonars

Science.gov (United States)

Lamarche, Geoffroy; Lurton, Xavier

2018-06-01

Multibeam echosounders are becoming widespread for the purposes of seafloor bathymetry mapping, but the acquisition and the use of seafloor backscatter measurements, acquired simultaneously with the bathymetric data, are still insufficiently understood, controlled and standardized. This presents an obstacle to well-accepted, standardized analysis and application by end users. The Marine Geological and Biological Habitat Mapping group (Geohab.org) has long recognized the need for better coherence and common agreement on acquisition, processing and interpretation of seafloor backscatter data, and established the Backscatter Working Group (BSWG) in May 2013. This paper presents an overview of this initiative, the mandate, structure and program of the working group, and a synopsis of the BSWG Guidelines and Recommendations to date. The paper includes (1) an overview of the current status in sensors and techniques available in seafloor backscatter data from multibeam sonars; (2) the presentation of the BSWG structure and results; (3) recommendations to operators, end-users, sonar manufacturers, and software developers using sonar backscatter for seafloor-mapping applications, for best practice methods and approaches for data acquisition and processing; and (4) a discussion on the development needs for future systems and data processing. We propose for the first time a nomenclature of backscatter processing levels that affords a means to accurately and efficiently describe the data processing status, and to facilitate comparisons of final products from various origins.

Evaluating multiple causes of persistent low microwave backscatter from Amazon forests after the 2005 drought

Science.gov (United States)

Hagen, Stephen; Braswell, Bobby; Milliman, Tom; Herrick, Christina; Peterson, Seth; Roberts, Dar; Keller, Michael; Palace, Michael

2017-01-01

Amazonia has experienced large-scale regional droughts that affect forest productivity and biomass stocks. Space-borne remote sensing provides basin-wide data on impacts of meteorological anomalies, an important complement to relatively limited ground observations across the Amazon’s vast and remote humid tropical forests. Morning overpass QuikScat Ku-band microwave backscatter from the forest canopy was anomalously low during the 2005 drought, relative to the full instrument record of 1999–2009, and low morning backscatter persisted for 2006–2009, after which the instrument failed. The persistent low backscatter has been suggested to be indicative of increased forest vulnerability to future drought. To better ascribe the cause of the low post-drought backscatter, we analyzed multiyear, gridded remote sensing data sets of precipitation, land surface temperature, forest cover and forest cover loss, and microwave backscatter over the 2005 drought region in the southwestern Amazon Basin (4°-12°S, 66°-76°W) and in adjacent 8°x10° regions to the north and east. We found moderate to weak correlations with the spatial distribution of persistent low backscatter for variables related to three groups of forest impacts: the 2005 drought itself, loss of forest cover, and warmer and drier dry seasons in the post-drought vs. the pre-drought years. However, these variables explained only about one quarter of the variability in depressed backscatter across the southwestern drought region. Our findings indicate that drought impact is a complex phenomenon and that better understanding can only come from more extensive ground data and/or analysis of frequent, spatially-comprehensive, high-resolution data or imagery before and after droughts. PMID:28873422

Stimulated brillouin backscatter of a short-pulse laser

International Nuclear Information System (INIS)

Hinkel, D.E.; Williams, E.A.; Berger, R.L.

1994-01-01

Stimulated Brillouin backscattering (SBBS) from a short-pulse laser, where the pulse length is short compared to the plasma length, is found to be qualitatively different than in the long pulse regime, where the pulse length is long compared to the plasma length. We find that after an initial transient of order the laser pulse length transit time, the instability reaches a steady state in the variables x' = x - V g t, t' = t, where V g is the pulse group velocity. In contrast, SBBS in a long pulse can be absolutely unstable and grows indefinitely, or until nonlinearities intervene. We find that the motion of the laser pulse induces Doppler related effects that substantially modify the backscattered spectrum at higher intensities, where the instability is strongly coupled (i.e. , has a growth rate large compared to the ion acoustic frequency)

Experimental evaluation of multiple Compton backscattering of gamma rays in copper

International Nuclear Information System (INIS)

Sabharwal, Arvind D.; Singh, Manpreet; Singh, Bhajan; Sandhu, B.S.

2009-01-01

The gamma ray photons continue to soften in energy as the number of scatterings increases in thick target, and results in the generation of singly and multiply scattered events. The number of these multiply scattered events increases with an increase in target thickness and saturates beyond a particular target thickness known as saturation depth. The present experiment is undertaken to study the saturation depth for 279 and 320 keV incident gamma ray photons multiply backscattered from copper targets of varying thickness. The backscattered photons are detected by a Nal(Tl) gamma detector whose pulse-height distribution is converted into a photon spectrum with the help of an inverse matrix approach. To extract the contribution of multiply backscattered photons only, the spectrum of singly scattered photon is reconstructed analytically. We observe that the numbers of multiply scattered events increases with an increase in target thickness and then saturate. The saturation depth is found to be decreasing with increase in incident gamma energy. (author)

Multiplex dipstick immunoassay for semi-quantitative determination of Fusarium mycotoxins in cereals

International Nuclear Information System (INIS)

Lattanzio, Veronica M.T.; Nivarlet, Noan; Lippolis, Vincenzo; Gatta, Stefania Della; Huet, Anne-Catherine; Delahaut, Philippe; Granier, Benoit; Visconti, Angelo

2012-01-01

Highlights: ► We developed a rapid method based on a multiplex dipstick immunoassay. ► The assay allowed the determination of major Fusarium toxins in wheat, oats, maize. ► We obtained cut off levels close to EU regulatory levels. - Abstract: A multiplex dipstick immunoassay based method for the simultaneous determination of major Fusarium toxins, namely zearalenone, T-2 and HT-2 toxins, deoxynivalenol and fumonisins in wheat, oats and maize has been developed. The dipstick format was based on an indirect competitive approach. Four test lines (mycotoxin–BSA conjugates) and one control line were located on the strip membrane. Labelled antibodies were freeze-dried within the microwell. Two matrix-related sample preparation protocols have been developed for wheat/oats (not containing fumonisins) and maize (containing fumonisins) respectively. The use of a methanol/water mixture for sample preparation allowed recoveries in the range 73–109% for all mycotoxins in all tested cereals, with relative standard deviation less than 10%. The optimized immunoassay was able to detect target mycotoxins at cut off levels equal to 80% of EU maximum permitted levels, i.e. 280, 400, 1400 and 3200 μg kg −1 , respectively, for zearalenone, T-2/HT-2 toxins, deoxynivalenol and fumonisins in maize, and 80, 400 and 1400 μg kg −1 , respectively, for zearalenone, T-2/HT-2 toxins and deoxynivalenol in wheat and oats. Analysis of naturally contaminated samples resulted in a good agreement between multiplex dipstick and validated confirmatory LC–MS/MS. The percentage of false positive results was less than or equal to 13%, whereas no false negative results were obtained. Data on the presence/absence of 6 mycotoxins at levels close to EU regulatory levels were obtained within 30 min. The proposed immunoassay protocol is rapid, inexpensive, easy-to-use and fit for purpose of rapid screening of mycotoxins in cereals.

Activity assays and immunoassays for plasma Renin and prorenin: information provided and precautions necessary for accurate measurement

DEFF Research Database (Denmark)

Campbell, Duncan J; Nussberger, Juerg; Stowasser, Michael

2009-01-01

into focus the differences in information provided by activity assays and immunoassays for renin and prorenin measurement and has drawn attention to the need for precautions to ensure their accurate measurement. CONTENT: Renin activity assays and immunoassays provide related but different information...... provided by these assays and of the precautions necessary to ensure their accuracy....

IFSA: a microfluidic chip-platform for frit-based immunoassay protocols

Science.gov (United States)

Hlawatsch, Nadine; Bangert, Michael; Miethe, Peter; Becker, Holger; Gärtner, Claudia

2013-03-01

Point-of-care diagnostics (POC) is one of the key application fields for lab-on-a-chip devices. While in recent years much of the work has concentrated on integrating complex molecular diagnostic assays onto a microfluidic device, there is a need to also put comparatively simple immunoassay-type protocols on a microfluidic platform. In this paper, we present the development of a microfluidic cartridge using an immunofiltration approach. In this method, the sandwich immunoassay takes place in a porous frit on which the antibodies have immobilized. The device is designed to be able to handle three samples in parallel and up to four analytical targets per sample. In order to meet the critical cost targets for the diagnostic market, the microfluidic chip has been designed and manufactured using high-volume manufacturing technologies in mind. Validation experiments show comparable sensitivities in comparison with conventional immunofiltration kits.

Evaluation of four immunoassays for diagnosis of brucellosis in Cuba

International Nuclear Information System (INIS)

Peraza, C.; Valdes, O.; Fonseca, N.; Garcia, M.; Alvarez, M.; Izquierdo, D.L.

1998-01-01

Four immunoassays (two indirect and two competitive ones) were evaluated by samples from areas free of disease, free by vaccination and affected areas using as reference techniques the Bengal Rose Tests, the Antigen in Buffered Plate Tests and the Complement Fixation Reaction Test. The evaluated samples demonstrated that the competitive assays (ELISAC-1 and ELISAC-2) detected less false positives than the indirect ones (ELISAI-1 and ELISAI-2). Of the competitive ELISAS, version 2 presented better sensitivity and specificity results in affected areas for 95% confidence: 80.9 - 96.9% and 97.5 - 99.4% respectively with positive predictive value in the range of 76 to 94% and negative predictive one between 98.1 and 99.7%. It was concluded that this assay can be used for brucellosis control because it gives higher assurance than the other evaluated immunoassays and it can discriminate infected from vaccinated animals. (author)

Analysis of soft magnetic materials by electron backscatter diffraction as a powerful tool

Science.gov (United States)

Schuller, David; Hohs, Dominic; Loeffler, Ralf; Bernthaler, Timo; Goll, Dagmar; Schneider, Gerhard

2018-04-01

The current work demonstrates that electron backscatter diffraction (EBSD) is a powerful and versatile characterization technique for investigating soft magnetic materials. The properties of soft magnets, e.g., magnetic losses strongly depend on the materials chemical composition and microstructure, including grain size and shape, texture, degree of plastic deformation and elastic strain. In electrical sheet stacks for e-motor applications, the quality of the machined edges/surfaces of each individual sheet is of special interest. Using EBSD, the influence of the punching process on the microstructure at the cutting edge is quantitatively assessed by evaluating the crystallographic misorientation distribution of the deformed grains. Using an industrial punching process, the maximum affected deformation depth is determined to be 200 - 300 μm. In the case of laser cutting, the affected deformation depth is determined to be approximately zero. Reliability and detection limits of the developed EBSD approach are evaluated on non-affected sample regions and model samples containing different indentation test bodies. A second application case is the investigation of the recrystallization process during the annealing step of soft magnetic composites (SMC) toroids produced by powder metallurgy as a function of compaction pressure, annealing parameters and powder particle size. With increasing pressure and temperature, the recrystallized area fraction (e.g., grains with crystallographic misorientations particle boundaries or areas with existing plastic deformation. The progress of recrystallization is visualized as a function of time and of different particle to grain size distributions. Here, large particles with coarse internal grain structures show a favorable recrystallization behavior which results in large bulk permeability of up to 600 - 700 and lower amount of residual misorientations (>3°).

Determination of digoxin by enzyme immunoassay and radioimmunoassay

International Nuclear Information System (INIS)

Mueller, H.; Braeuer, H.; Foerster, G.; Reinhardt, M.

1978-01-01

The results of parallel determinations of digoxin in the sera of non selected patients (n=104) by enzyme immunoassay (EMIT.EIA) and radioimmunoassay (J-125 labeled RIA) were compared with each other. The determinations revealed considerably different concentrations; the values determined by EIA were statistical lower (for EIA 1,09+'0,99ng/ml, for RIA 1,34+'1,01ng/ml, p [de

A study using an isotope probe comparing immunoassay with serology in detection of Brucella Abortus antibody

International Nuclear Information System (INIS)

Devlin, J.G.; Redington, F.; Stephenson, M.

1986-01-01

We report a comparison of radio-immunoassay with conventional serology in the detection of brucella abortus antibody from three laboratories. Overall agreement by Chi squared analysis is 5%. There are significant differences between laboratories and a significant number of sero negative suspect sera (from 20% - 60%) were positive by ratio-immunoassay test. We suspect that conventional serology under-reports the incidence of antibody to brucella abortus. (author)

Incidence angle normalization of radar backscatter data

Science.gov (United States)

NASA’s Soil Moisture Passive Active (SMAP) satellite (~2014) will include a radar system that will provide L-band multi-polarization backscatter at a constant incidence angle of 40º. During the pre-launch phase of the project there is a need for observations that will support the radar-based soil mo...

Lattice constant measurement from electron backscatter diffraction patterns

DEFF Research Database (Denmark)

Saowadee, Nath; Agersted, Karsten; Bowen, Jacob R.

2017-01-01

Kikuchi bands in election backscattered diffraction patterns (EBSP) contain information about lattice constants of crystallographic samples that can be extracted via the Bragg equation. An advantage of lattice constant measurement from EBSPs over diffraction (XRD) is the ability to perform local ...

About the information depth of backscattered electron imaging

Czech Academy of Sciences Publication Activity Database

Piňos, Jakub; Mikmeková, Šárka; Frank, Luděk

2017-01-01

Roč. 266, č. 3 (2017), s. 335-342 ISSN 0022-2720 Institutional support: RVO:68081731 Keywords : backscattered electrons * information depth * penetration of electrons Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering OBOR OECD: Materials engineering Impact factor: 1.692, year: 2016

Ultrasonic Characterization of Tissues via Backscatter Frequency Dependence

DEFF Research Database (Denmark)

Stetson, Paul F.; Sommer, F.G.

1997-01-01

, significantly lower mean frequency of ultrasound backscattered from cirrhotic, compared to normal, liver tissue was noted, Studies of benign and malignant liver tumors (hemangiomas and metastases, respectively) indicated differences in frequency content of these tumors, compared to the adjacent normal liver...

Optimization of phantom backscatter thickness and lateral scatter volume for radiographic film dosimetry

International Nuclear Information System (INIS)

Srivastava, R.P.; De Wagter, C.

2012-01-01

The aim of this study is to determine the optimal backscatter thickness and lateral phantom dimension beyond the irradiated volume for the dosimetric verification with radiographic film when applying large field sizes. Polystyrene and Virtual Water™ phantoms were used to study the influence of the phantom backscatter thickness. EDR2 and XV films were used in 6 and 18 MV photon beams. The results show 11.4% and 6.4% over-response of the XV2 film when compared to the ion chamber for 6 MV 30×30 and 10×10 cm 2 field sizes, respectively, when the phantom backscatter thickness is 5 cm. For the same setup, measurements with EDR2 films indicate 8.5% and 1.7% over-response. The XV2 film response in the polystyrene phantom is about 2.0% higher than in the Virtual Water™ phantom for the 6 MV beam and 20 cm backscatter thickness. Similar results were obtained for EDR2 film. In the lateral scatter study, film response was nearly constant within 5 cm of lateral thickness and it increases when lateral thickness increases due to more multiple scatter of low energy photons. The backscatter thickness of the phantom should be kept below 7 cm for the accuracy of the film dosimetry. The lateral extension of the phantom should not be more than 5 cm from the field boundary in case of large irradiated volumes.

X-ray backscatter radiography. Intrusive instead of penetrating, X-ray shadow phenomenon

International Nuclear Information System (INIS)

Wrobel, Norma; Kolkoori, Sanjeevareddy; Osterloh, Kurt; European Federation for Non-Destructive Testing

2013-01-01

Generally, the primary practical advantage of X-ray backscattering radiography is that there is no need to place a detector on the side of the specimen opposite to the source. Such a situation usually is encountered whenever the specimen is not only standing right in front of a wall or even inside a wall but also if the specimen is such big that radiography is not possible because of the layer thickness to be penetrated. The method used here differs fundamentally from the conventional method to interrogate the object with a scanning beam ('pencil beam') and to collect the whole backscattered radiation from the area. The object is fully illuminated by a (uncollimated) cone beam. Here, the image is recorded with a camera of absorbent material (tungsten, lead), which contains a matrix detector as the image receiver. The optical effect is generated by a special twisted slit collimator which operates according to an extended pinhole camera. The independent positioning of source and camera allows a variable irradiation geometry which causes different images as a result. As a consequence, a complex object in front of a backscattering wall appears completely different than standing alone. So X-ray backscatter images have to be interpreted according to their illumination with X-rays and their surroundings. (orig.)

A Novel Colorimetric Immunoassay Utilizing the Peroxidase Mimicking Activity of Magnetic Nanoparticles

Directory of Open Access Journals (Sweden)

Hyun Gyu Park

2013-05-01

Full Text Available A simple colorimetric immunoassay system, based on the peroxidase mimicking activity of Fe3O4 magnetic nanoparticles (MNPs, has been developed to detect clinically important antigenic molecules. MNPs with ca. 10 nm in diameter were synthesized and conjugated with specific antibodies against target molecules, such as rotaviruses and breast cancer cells. Conjugation of the MNPs with antibodies (MNP-Abs enabled specific recognition of the corresponding target antigenic molecules through the generation of color signals arising from the colorimetric reaction between the selected peroxidase substrate, 3,3',5,5'-tetramethylbenzidine (TMB and H2O2. Based on the MNP-promoted colorimetric reaction, the target molecules were detected and quantified by measuring absorbance intensities corresponding to the oxidized form of TMB. Owing to the higher stabilities and economic feasibilities of MNPs as compared to horseradish peroxidase (HRP, the new colorimetric system employing MNP-Abs has the potential of serving as a potent immunoassay that should substitute for conventional HRP-based immunoassays. The strategy employed to develop the new methodology has the potential of being extended to the construction of simple diagnostic systems for a variety of biomolecules related to human cancers and infectious diseases, particularly in the realm of point-of-care applications.

Fast and sensitive detection of enteropathogenic Yersinia by immunoassays.

Science.gov (United States)

Laporte, Jérôme; Savin, Cyril; Lamourette, Patricia; Devilliers, Karine; Volland, Hervé; Carniel, Elisabeth; Créminon, Christophe; Simon, Stéphanie

2015-01-01

Yersinia enterocolitica and Yersinia pseudotuberculosis, the two Yersinia species that are enteropathogenic for humans, are distributed worldwide and frequently cause diarrhea in inhabitants of temperate and cold countries. Y. enterocolitica is a major cause of foodborne disease resulting from consumption of contaminated pork meat and is further associated with substantial economic cost. However, investigation of enteropathogenic Yersinia species is infrequently performed routinely in clinical laboratories because of their specific growth characteristics, which make difficult their isolation from stool samples. Moreover, current isolation procedures are time-consuming and expensive, thus leading to underestimates of the incidence of enteric yersiniosis, inappropriate prescriptions of antibiotic treatments, and unnecessary appendectomies. The main objective of the study was to develop fast, sensitive, specific, and easy-to-use immunoassays, useful for both human and veterinary diagnosis. Monoclonal antibodies (MAbs) directed against Y. enterocolitica bioserotypes 2/O:9 and 4/O:3 and Y. pseudotuberculosis serotypes I and III were produced. Pairs of MAbs were selected by testing their specificity and affinity for enteropathogenic Yersinia and other commonly found enterobacteria. Pairs of MAbs were selected to develop highly sensitive enzyme immunoassays (EIAs) and lateral flow immunoassays (LFIs or dipsticks) convenient for the purpose of rapid diagnosis. The limit of detection of the EIAs ranged from 3.2 × 10(3) CFU/ml to 8.8 × 10(4) CFU/ml for pathogenic serotypes I and III of Y. pseudotuberculosis and pathogenic bioserotypes 2/O:9 and 4/O:3 of Y. enterocolitica and for the LFIs ranged from 10(5) CFU/ml to 10(6) CFU/ml. A similar limit of detection was observed for artificially contaminated human feces. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

Kinase Activity Studied in Living Cells Using an Immunoassay

Science.gov (United States)

Bavec, Aljos?a

2014-01-01

This laboratory exercise demonstrates the use of an immunoassay for studying kinase enzyme activity in living cells. The advantage over the classical method, in which students have to isolate the enzyme from cell material and measure its activity in vitro, is that enzyme activity is modulated and measured in living cells, providing a more…

Immunoassay for Capsular Antigen of Bacillus anthracis Enables Rapid Diagnosis in a Rabbit Model of Inhalational Anthrax.

Directory of Open Access Journals (Sweden)

Marcellene A Gates-Hollingsworth

Full Text Available Inhalational anthrax is a serious biothreat. Effective antibiotic treatment of inhalational anthrax requires early diagnosis; the further the disease has progressed, the less the likelihood for cure. Current means for diagnosis such as blood culture require several days to a result and require advanced laboratory infrastructure. An alternative approach to diagnosis is detection of a Bacillus anthracis antigen that is shed into blood and can be detected by rapid immunoassay. The goal of the study was to evaluate detection of poly-γ-D-glutamic acid (PGA, the capsular antigen of B. anthracis, as a biomarker surrogate for blood culture in a rabbit model of inhalational anthrax. The mean time to a positive blood culture was 26 ± 5.7 h (mean ± standard deviation, whereas the mean time to a positive ELISA was 22 ± 4.2 h; P = 0.005 in comparison with blood culture. A lateral flow immunoassay was constructed for detection of PGA in plasma at concentrations of less than 1 ng PGA/ml. Use of the lateral flow immunoassay for detection of PGA in the rabbit model found that antigen was detected somewhat earlier than the earliest time point at which the blood culture became positive. The low cost, ease of use, and rapid time to result of the lateral flow immunoassay format make an immunoassay for PGA a viable surrogate for blood culture for detection of infection in individuals who have a likelihood of exposure to B. anthracis.

Rocket measurements of energetic particles in the midlatitude precipitation zone

Science.gov (United States)

Voss, H. D.; Smith, L. G.; Braswell, F. M.

1980-01-01

Measurements of energetic ion and electron properties as a function of altitude in the midlatitude zone of nighttime energetic particle precipitation are reported. The measurements of particle fluxes, energy spectra and pitch angle distributions were obtained by a Langmuir probe, six energetic particle spectrometers and an electrostatic analyzer on board a Nike Apache rocket launched near the center of the midlatitude zone during disturbed conditions. It is found that the incident flux was primarily absorbed rather than backscattered, and consists of mainly energetic hydrogen together with some helium and a small energetic electron component. Observed differential energy spectra of protons having an exponential energy spectrum, and pitch angle distributions at various altitudes indicate that the energetic particle flux decreases rapidly for pitch angles less than 70 deg. An energetic particle energy flux of 0.002 ergs/sq cm per sec is calculated which indicates the significance of energetic particles as a primary nighttime ionization source for altitudes between 120 and 200 km in the midlatitude precipitation zone.

Improved detection and mapping of deepwater hydrocarbon seeps: optimizing multibeam echosounder seafloor backscatter acquisition and processing techniques

Science.gov (United States)

Mitchell, Garrett A.; Orange, Daniel L.; Gharib, Jamshid J.; Kennedy, Paul

2018-02-01

Marine seep hunting surveys are a current focus of hydrocarbon exploration surveys due to recent advances in offshore geophysical surveying, geochemical sampling, and analytical technologies. Hydrocarbon seeps are ephemeral, small, discrete, and therefore difficult to sample on the deep seafloor. Multibeam echosounders are an efficient seafloor exploration tool to remotely locate and map seep features. Geophysical signatures from hydrocarbon seeps are acoustically-evident in bathymetric, seafloor backscatter, midwater backscatter datasets. Interpretation of these signatures in backscatter datasets is a fundamental component of commercial seep hunting campaigns. Degradation of backscatter datasets resulting from environmental, geometric, and system noise can interfere with the detection and delineation of seeps. We present a relative backscatter intensity normalization method and an oversampling acquisition technique that can improve the geological resolvability of hydrocarbon seeps. We use Green Canyon (GC) Block 600 in the Northern Gulf of Mexico as a seep calibration site for a Kongsberg EM302 30 kHz MBES prior to the start of the Gigante seep hunting program to analyze these techniques. At GC600, we evaluate the results of a backscatter intensity normalization, assess the effectiveness of 2X seafloor coverage in resolving seep-related features in backscatter data, and determine the off-nadir detection limits of bubble plumes using the EM302. Incorporating these techniques into seep hunting surveys can improve the detectability and sampling of seafloor seeps.

Improved detection and mapping of deepwater hydrocarbon seeps: optimizing multibeam echosounder seafloor backscatter acquisition and processing techniques

Science.gov (United States)

Mitchell, Garrett A.; Orange, Daniel L.; Gharib, Jamshid J.; Kennedy, Paul

2018-06-01

Marine seep hunting surveys are a current focus of hydrocarbon exploration surveys due to recent advances in offshore geophysical surveying, geochemical sampling, and analytical technologies. Hydrocarbon seeps are ephemeral, small, discrete, and therefore difficult to sample on the deep seafloor. Multibeam echosounders are an efficient seafloor exploration tool to remotely locate and map seep features. Geophysical signatures from hydrocarbon seeps are acoustically-evident in bathymetric, seafloor backscatter, midwater backscatter datasets. Interpretation of these signatures in backscatter datasets is a fundamental component of commercial seep hunting campaigns. Degradation of backscatter datasets resulting from environmental, geometric, and system noise can interfere with the detection and delineation of seeps. We present a relative backscatter intensity normalization method and an oversampling acquisition technique that can improve the geological resolvability of hydrocarbon seeps. We use Green Canyon (GC) Block 600 in the Northern Gulf of Mexico as a seep calibration site for a Kongsberg EM302 30 kHz MBES prior to the start of the Gigante seep hunting program to analyze these techniques. At GC600, we evaluate the results of a backscatter intensity normalization, assess the effectiveness of 2X seafloor coverage in resolving seep-related features in backscatter data, and determine the off-nadir detection limits of bubble plumes using the EM302. Incorporating these techniques into seep hunting surveys can improve the detectability and sampling of seafloor seeps.

Interferometric evidence for the observation of ground backscatter originating behind the CUTLASS coherent HF radars

Science.gov (United States)

Milan, S. E.; Jones, T. B.; Robinson, T. R.; Thomas, E. C.; Yeoman, T. K.

1997-01-01

Interferometric techniques allow the SuperDARN coherent HF radars to determine the elevation angles of returned backscatter, giving information on the altitude of the scatter volume, in the case of ionospheric backscatter, or the reflection altitude, in the case of ground backscatter. Assumptions have to be made in the determination of elevation angles, including the direction of arrival, or azimuth, of the returned signals, usually taken to be the forward look-direction (north) of the radars, specified by the phasing of the antenna arrays. It is shown that this assumption is not always valid in the case of ground backscatter, and that significant returns can be detected from the backward look-direction of the radars. The response of the interferometer to backscatter from behind the radar is modelled and compared with observations. It is found that ground backscatter from a field-of-view that is the mirror image of the forward-looking field-of-view is a common feature of the observations, and this interpretation successfully explains several anomalies in the received backscatter. Acknowledgements. The authors are grateful to Prof. D. J. Southwood (Imperial College, London), J. C. Samson (University of Alberta, Edmonton), L. J. Lanzerotti (AT&T Bell Laboratories), A. Wolfe (New York City Technical College) and to Dr. M. Vellante (University of LÁquila) for helpful discussions. They also thank Dr. A. Meloni (Istituto Nazionale di Geofisica, Roma) who made available geomagnetic field observations from LÁquila Geomagnetic Observatory. This research activity at LÁquila is supported by MURST (40% and 60% contracts) and by GIFCO/CNR. Topical Editor K.-H. Glaßmeier thanks C. Waters and S. Fujita for their help in evaluating this paper.-> Correspondence to :P. Francia->

Method and Apparatus for Computed Imaging Backscatter Radiography

Science.gov (United States)

Shedlock, Daniel (Inventor); Meng, Christopher (Inventor); Sabri, Nissia (Inventor); Dugan, Edward T. (Inventor); Jacobs, Alan M. (Inventor)

2013-01-01

Systems and methods of x-ray backscatter radiography are provided. A single-sided, non-destructive imaging technique utilizing x-ray radiation to image subsurface features is disclosed, capable of scanning a region using a fan beam aperture and gathering data using rotational motion.

Massively multi-parametric immunoassays using ICPMS

International Nuclear Information System (INIS)

Tanner, S.D.; Ornatsky, O.; Bandura, D.R.; Baranov, V.I.

2009-01-01

The use of stable isotopes as tags in immunoassays, and their determination by ICPMS, is poised to have a huge impact on multi-parametric bioanalysis. A new technology, which we term 'mass cytometry', enables high throughput, highly multiplexed individual cell analysis. Preliminary results for T-cell immunophenotyping in peripheral blood mononuclear cells (PBMC), agonist influence on concomitant phosphorylation pathways, and sub-classification of acute myeloid leukemia patients' samples will be presented. The significance of individual cell analysis is demonstrated by the identification of populations of rogue cells in PBMC samples through the use of multidimensional neural network cluster analysis. (author)

False biochemical diagnosis of hyperthyroidism in streptavidin-biotin-based immunoassays: the problem of biotin intake and related interferences.

Science.gov (United States)

Piketty, Marie-Liesse; Polak, Michel; Flechtner, Isabelle; Gonzales-Briceño, Laura; Souberbielle, Jean-Claude

2017-05-01

Immunoassays are now commonly used for hormone measurement, in high throughput analytical platforms. Immunoassays are generally robust to interference. However, endogenous analytical error may occur in some patients; this may be encountered in biotin supplementation or in the presence of anti-streptavidin antibody, in immunoassays involving streptavidin-biotin interaction. In these cases, the interference may induce both false positive and false negative results, and simulate a seemingly coherent hormonal profile. It is to be feared that this type of errors will be more frequently observed. This review underlines the importance of keeping close interactions between biologists and clinicians to be able to correlate the hormonal assay results with the clinical picture.

Targeted selected reaction monitoring mass spectrometric immunoassay for insulin-like growth factor 1.

Directory of Open Access Journals (Sweden)

Eric E Niederkofler

Full Text Available Insulin-like growth factor 1 (IGF1 is an important biomarker of human growth disorders that is routinely analyzed in clinical laboratories. Mass spectrometry-based workflows offer a viable alternative to standard IGF1 immunoassays, which utilize various pre-analytical preparation strategies. In this work we developed an assay that incorporates a novel sample preparation method for dissociating IGF1 from its binding proteins. The workflow also includes an immunoaffinity step using antibody-derivatized pipette tips, followed by elution, trypsin digestion, and LC-MS/MS separation and detection of the signature peptides in a selected reaction monitoring (SRM mode. The resulting quantitative mass spectrometric immunoassay (MSIA exhibited good linearity in the range of 1 to 1,500 ng/mL IGF1, intra- and inter-assay precision with CVs of less than 10%, and lowest limits of detection of 1 ng/mL. The linearity and recovery characteristics of the assay were also established, and the new method compared to a commercially available immunoassay using a large cohort of human serum samples. The IGF1 SRM MSIA is well suited for use in clinical laboratories.

In vitro conditions modify immunoassayability of bovine pituitary prolactin and growth hormone: insights into their secretory granule storage forms

International Nuclear Information System (INIS)

Lorenson, M.Y.

1985-01-01

The amount of immunoassayable intracellular bovine (b) PRL and GH varies depending on treatment conditions. The present studies were designed to characterize the mechanisms involved and to compare immunoassayability of both hormones under similar conditions. Pituitary homogenate and secretory granule hormones displayed both time- and temperature-dependent increases when incubated at pH 10.5 with reduced glutathione. Changes in immunoassayability seem to reflect conversion from poorly immunoactive tissue hormone oligomers to monomeric hormone. The data indicate that oligomeric bPRL is stabilized primarily by intermolecular disulfide bonds, although it is also susceptible to urea, SDS, and EDTA; granule thiols may also influence the conversion to monomer. The storage form of bGH appears to be stabilized differently. Maneuvers demonstrated in these studies to influence immunoassayability correlate very well with their previously established effects on hormone release and secretion, strengthening the likelihood that a functional link exists between assayability and secretion

High-frequency attenuation and backscatter measurements of rat blood between 30 and 60 MHz

International Nuclear Information System (INIS)

Huang, Chih-Chung

2010-01-01

There has recently been a great deal of interest in noninvasive high-frequency ultrasound imaging of small animals such as rats due to their being the preferred animal model for gene therapy and cancer research. Improving the interpretation of the obtained images and furthering the development of the imaging devices require a detailed knowledge of the ultrasound attenuation and backscattering of biological tissue (e.g. blood) at high frequencies. In the present study, the attenuation and backscattering coefficients of the rat red blood cell (RBC) suspensions and whole blood with hematocrits ranging from 6% to 40% were measured between 30 and 60 MHz using a modified substitution approach. The acoustic parameters of porcine blood under the same conditions were also measured in order to compare differences in the blood properties between these two animals. For porcine blood, both whole blood and RBC suspension were stirred at a rotation speed of 200 rpm. Three different rotation speeds of 100, 200 and 300 rpm were carried out for rat blood experiments. The attenuation coefficients of both rat and porcine blood were found to increase linearly with frequency and hematocrit (the values of coefficients of determination (r 2 ) are around 0.82-0.97 for all cases). The average attenuation coefficient of rat whole blood with a hematocrit of 40% increased from 0.26 Nepers mm -1 at 30 MHz to 0.47 Nepers mm -1 at 60 MHz. The maximum backscattering coefficients of both rat and porcine RBC suspensions were between 10% and 15% hematocrits at all frequencies. The fourth-power dependence of backscatter on frequency was approximately valid for rat RBC suspensions with hematocrits between 6% and 40%. However, the frequency dependence of the backscatter estimate deviates from a fourth-power law for porcine RBC suspension with hematocrit higher than 20%. The backscattering coefficient plateaued for hematocrits higher than 15% in porcine blood, but for rat blood it was maximal around a

340 nm pulsed UV LED system for europium-based time-resolved fluorescence detection of immunoassays

OpenAIRE

Rodenko, Olga; Fodgaard, Henrik; Tidemand-Lichtenberg, Peter; Petersen, Paul Michael; Pedersen, Christian

2016-01-01

We report on the design, development and investigation of an optical system based on UV light emitting diode (LED) excitation at 340 nm for time-resolved fluorescence detection of immunoassays. The system was tested to measure cardiac marker Troponin I with a concentration of 200 ng/L in immunoassay. The signal-to-noise ratio was comparable to state-of-the-art Xenon flash lamp based unit with equal excitation energy and without overdriving the LED. We performed a comparative study of the flas...

User expectations for multibeam echo sounders backscatter strength data-looking back into the future

Science.gov (United States)

Lucieer, Vanessa; Roche, Marc; Degrendele, Koen; Malik, Mashkoor; Dolan, Margaret; Lamarche, Geoffroy

2018-06-01

With the ability of multibeam echo sounders (MBES) to measure backscatter strength (BS) as a function of true angle of insonification across the seafloor, came a new recognition of the potential of backscatter measurements to remotely characterize the properties of the seafloor. Advances in transducer design, digital electronics, signal processing capabilities, navigation, and graphic display devices, have improved the resolution and particularly the dynamic range available to sonar and processing software manufacturers. Alongside these improvements the expectations of what the data can deliver has also grown. In this paper, we identify these user-expectations and explore how MBES backscatter is utilized by different communities involved in marine seabed research at present, and the aspirations that these communities have for the data in the future. The results presented here are based on a user survey conducted by the GeoHab (Marine Geological and Biological Habitat Mapping) association. This paper summarises the different processing procedures employed to extract useful information from MBES backscatter data and the various intentions for which the user community collect the data. We show how a range of backscatter output products are generated from the different processing procedures, and how these results are taken up by different scientific disciplines, and also identify common constraints in handling MBES BS data. Finally, we outline our expectations for the future of this unique and important data source for seafloor mapping and characterisation.

Multi-Polarization ASAR Backscattering from Herbaceous Wetlands in Poyang Lake Region, China

Directory of Open Access Journals (Sweden)

Huiyong Sang

2014-05-01

Full Text Available Wetlands are one of the most important ecosystems on Earth. There is an urgent need to quantify the biophysical parameters (e.g., plant height, aboveground biomass and map total remaining areas of wetlands in order to evaluate the ecological status of wetlands. In this study, Environmental Satellite/Advanced Synthetic Aperture Radar (ENVISAT/ASAR dual-polarization C-band data acquired in 2005 is tested to investigate radar backscattering mechanisms with the variation of hydrological conditions during the growing cycle of two types of herbaceous wetland species, which colonize lake borders with different elevation in Poyang Lake region, China. Phragmites communis (L. Trin. is semi-aquatic emergent vegetation with vertical stem and blade-like leaves, and the emergent Carex spp. has rhizome and long leaves. In this study, the potential of ASAR data in HH-, HV-, and VV-polarization in mapping different wetland types is examined, by observing their dynamic variations throughout the whole flooding cycle. The sensitivity of ASAR backscattering coefficients to vegetation parameters of plant height, fresh and dry biomass, and vegetation water content is also analyzed for Phragmites communis (L. Trin. and Carex spp. The research for Phragmites communis (L. Trin. shows that HH polarization is more sensitive to plant height and dry biomass than HV polarization. ASAR backscattering coefficients are relatively less sensitive to fresh biomass, especially in HV polarization. However, both are highly dependent on canopy water content. In contrast, the dependence of HH- and HV- backscattering from Carex community on vegetation parameters is poor, and the radar backscattering mechanism is controlled by ground water level.

Ion backscattering and X-ray investigations of violin varnish and wood

International Nuclear Information System (INIS)

Sigurd, D.; Tove, P.A.; Petersson, S.

1980-01-01

The use of Rutherford backscattering (RBS) of ions (i.e. α-particles) and excitation of characteristic X-rays by protons, for determining the elemental composition of the top layers of violin varnish and wood is described. The amount of elements such as C, O, Si, S, Ca, Cu, Fe, Ni, Zn, Pb has been measured, on the varnished and unvarnished sides of wood samples from old instrument makers such as A. Stradivari, G. Guarneri del Gesu, Santo Serafin, C. Tononi etc. Differences in both the shape of the RBS spectra and in the presence of different elements are found and discussed. The use of X-ray excitation by ions (i.e. protons) helps in assessing the presence of the different elements. X-ray investigations can be done with instruments in air while RBS (at least in the presently used form) has to be performed in a vacuum chamber. Apart from offering an aid in determining the materials and procedures which were used in fabricating acoustically excellent instruments, the method could be of value for identifying their authenticity. (orig.)

Ion backscattering and x-ray investigations of violin varnish and wood

Science.gov (United States)

Arne Tove, Per; Sigurd, Dag; Petersson, Sture

1980-01-01

The use of Rutherford backscattering (RBS) of ions (i.e. α-particles) and excitation of characteristic X-rays by protons, for determining the elemental composition of the top layers of violin varnish and wood is described. The amount of elements such as C, O, Si, S, Ca, Cu, Fe, Ni, Zn, Pb has been measured, on the varnished and unvarnished sides of wood samples from old instrument makers such as A. Stradivari, G. Guarneri del Gesu, Santo Serafin, C. Tononi etc. Differences in both the shape of the RBS spectra and in the presence of different elements are found and discussed. The use of X-ray excitation by ions (i.e. protons) helps in assessing the presence of the different elements. X-ray investigations can be done with instruments in air while RBS (at least in the presently used form) has to be performed in a vacuum chamber. Apart from offering an aid in determining the materials and procedures which were used in fabricating acoustically excellent instruments, the method could be of value for identifying their authenticity.

Application of resonant backscattering spectrometry for determination of pore structure changes

Energy Technology Data Exchange (ETDEWEB)

Paszti, F. E-mail: paszti@rmki.kfki.hu; Szilagyi, E.; Manuaba, A.; Battistig, G

2000-03-01

In the present work slightly oxidised porous silicon samples of columnar type were investigated by resonant elastic scattering using the 3045 keV resonance in the {sup 16}O({alpha},{alpha}){sup 16}O reaction. If the incident energy is slightly above the resonance energy, a characteristic peak appears in the energy spectra of the backscattered particles. In porous material the individual ions travel fluctuating distance in pores, hence, the peak width depends on the structure of the sample. When changing the direction of the analysing beam to the sample, the width of the resonance peak changes in a special way characterising the angular distribution of the pore walls. This resonance method was applied to columnar type porous Si samples implanted by 4 MeV N ions at various tilt angles and fluences. It was shown that the beam tilts the pore walls by an angle proportional to the fluence and the sine of the angle between the beam and the pore walls. Meantime, the angular divergence of the pore walls also increases. The underlying mechanism is briefly discussed.

Application of neutron backscatter techniques to level measurement problems

International Nuclear Information System (INIS)

Leonardi-Cattolica, A.M.; McMillan, D.H.; Telfer, A.; Griffin, L.H.; Hunt, R.H.

1982-01-01

We have designed and built portable level detectors and fixed level monitors based on neutron scattering and detection principles. The main components of these devices, which we call neutron backscatter gauges, are a neutron emitting radioisotope, a neutron detector, and a ratemeter. The gauge is a good detector for hydrogen but is much less sensitive to most other materials. This allows level measurements of hydrogen bearing materials, such as hydrocarbons, to be made through the walls of metal vessels. Measurements can be made conveniently through steel walls which are a few inches thick. We have used neutron backscatter gauges in a wide variety of level measurement applications encountered in the petrochemical industry. In a number of cases, the neutron techniques have proven to be superior to conventional level measurement methods, including gamma ray methods

Backscattering analysis of high frequency ultrasonic imaging for ultrasound-guided breast biopsy

Science.gov (United States)

Cummins, Thomas; Akiyama, Takahiro; Lee, Changyang; Martin, Sue E.; Shung, K. Kirk

2017-03-01

A new ultrasound-guided breast biopsy technique is proposed. The technique utilizes conventional ultrasound guidance coupled with a high frequency embedded ultrasound array located within the biopsy needle to improve the accuracy in breast cancer diagnosis.1 The array within the needle is intended to be used to detect micro- calcifications indicative of early breast cancers such as ductal carcinoma in situ (DCIS). Backscattering analysis has the potential to characterize tissues to improve localization of lesions. This paper describes initial results of the application of backscattering analysis of breast biopsy tissue specimens and shows the usefulness of high frequency ultrasound for the new biopsy related technique. Ultrasound echoes of ex-vivo breast biopsy tissue specimens were acquired by using a single-element transducer with a bandwidth from 41 MHz to 88 MHz utilizing a UBM methodology, and the backscattering coefficients were calculated. These values as well as B-mode image data were mapped in 2D and matched with each pathology image for the identification of tissue type for the comparison to the pathology images corresponding to each plane. Microcalcifications were significantly distinguished from normal tissue. Adenocarcinoma was also successfully differentiated from adipose tissue. These results indicate that backscattering analysis is able to quantitatively distinguish tissues into normal and abnormal, which should help radiologists locate abnormal areas during the proposed ultrasound-guided breast biopsy with high frequency ultrasound.

Nanobody-based electrochemical immunoassay for Bacillus thuringiensis Cry1Ab toxin by detecting the enzymatic formation of polyaniline

International Nuclear Information System (INIS)

Zhu, Min; Li, Guanghui; Li, Min; Zhou, Zikai; Liu, Hong; Lei, Hongtao; Shen, Yanfei; Wan, Yakun

2015-01-01

We describe an electrochemical immunoassay for the Cry1Ab toxin that is produced by Bacillus thuringiensis. It is making use of a nanobody (a heavy-chain only antibody) that was selected from an immune phage displayed library. A biotinylated primary nanobody and a HRP-conjugated secondary nanobody were applied in a sandwich immunoassay where horseradish peroxidase (HRP) is used to produce polyaniline (PANI) from aniline. PANI can be easily detected by differential pulse voltammetry at a working voltage as low as 40 mV (vs. Ag/AgCl) which makes the assay fairly selective. This immunoassay for Cry1Ab has an analytical range from 0.1 to 1000 ng∙mL -1 and a 0.07 ng∙mL -1 lower limit of detection. The average recoveries of the toxin from spiked samples are in the range from 102 to 114 %, with a relative standard deviation of <7.5 %. The results demonstrated that the assay represented an attractive alternative to existing immunoassays in enabling affordable, sensitive, robust and specific determination of this toxin. (author)

Determination of particles concentration in Black Sea waters from spectral beam attenuation coefficient

Science.gov (United States)

Korchemkina, E. N.; Latushkin, A. A.; Lee, M. E.

2017-11-01

The methods of determination of concentration and scattering by suspended particles in seawater are compared. The methods considered include gravimetric measurements of the mass concentration of suspended matter, empirical and analytical calculations based on measurements of the light beam attenuation coefficient (BAC) in 4 spectral bands, calculation of backscattering by particles using satellite measurements in the visible spectral range. The data were obtained in two cruises of the R/V "Professor Vodyanitsky" in the deep-water part of the Black Sea in July and October 2016., Spatial distribution of scattering by marine particles according to satellite data is in good agreement with the contact measurements.

Probing insect backscatter cross section and melanization using kHz optical remote detection system

Science.gov (United States)

Gebru, Alem; Brydegaard, Mikkel; Rohwer, Erich; Neethling, Pieter

2017-01-01

A kHz optical remote sensing system is implemented to determine insect melanization features. This is done by measuring the backscatter signal in the visible and near-infrared (VIS-NIR) and short-wave infrared (SWIR) in situ. It is shown that backscatter cross section in the SWIR is insensitive to melanization and absolute melanization can be derived from the ratio of backscatter cross section of different bands (SWIR/VIS-NIR). We have shown that reflectance from insect is stronger in the SWIR as compared to NIR and VIS. This reveals that melanization plays a big role to determine backscatter cross section. One can use this feature as a tool to improve insect species and age classification. To support the findings, we illustrated melanization feature using three different insects [dead, dried specimens of snow white moth (Spilosoma genus), fox moth (Macrothylacia), and leather beetle (Odontotaenius genus)]. It is shown that reflectance from the leather beetle in the VIS and NIR is more affected by melanization as compared with snow white moth.

Backscattering study and theoretical investigation of planar channeling processes. I. Experimental results

International Nuclear Information System (INIS)

Abel, F.; Amsel, G.; Bruneaux, M.; Cohen, C.; L'Hoir, A.

1975-01-01

Backscattering experiments in planar channeling have been performed on iron single crystals with 1.9-MeV 4 He beams; these conditions having been chosen for optimal study of the structure of the spectra. Both for the (110) and (100) planes five equally spaced yield maxima are clearly resolved, the maxima damping out at lower energies. Spectra were also registered at various angles of incidence phi 0 with respect to the planes. Yield maxima are observed up to values of phi 0 twice the half-width at half-minimum psi 1 / 2 of an angular scan across the plane psi 1 / 2 =18' for the (110) plane. Except for the first two peaks, these maxima have the same spacing as in the aligned spectrum. They appear to be due to particles belonging to a well-defined transverse energy interval. The mean stopping power for these particles is close to the random stopping power and the mean half-wavelength of their oscillating trajectories in the planar channels calculated from the results is lambda=380 A for the (110) plane and lambda=320 A for the (100) plane. For phi 0 >1.2psi 1 / 2 , the yield on the first maximum is greater than the random yield, reaching approx.1.6 times the latter for 1.4psi 1 / 2 0 1 / 2 . The shoulder effect in the angular scans, as observed for various depths, is clearly related to the yield maxima and hence depends strongly on the position and width of the depth interval chosen. The meaning and validity of the assumption of statistical equilibrium for planar channeled particles are discussed in light of the results

Highly sensitive electrochemical immunoassay for human IgG using double-encoded magnetic redox-active nanoparticles

International Nuclear Information System (INIS)

Tang, D.; Tang, J.; Su, B.; Chen, H.; Chen, G.; Huang, J.

2010-01-01

A new sandwich-type electrochemical immunoassay was developed for the detection of human IgG using doubly-encoded and magnetic redox-active nanoparticles as recognition elements on the surface of a glassy carbon electrode modified with anti-IgG on nanogold particles. The recognition elements were synthesized by coating magnetic Fe3O4 nanoparticles with Prussian blue nanoparticles and then covered with peroxidase-labeled anti-IgG antibodies (POx-anti-IgG) on Prussian blue nanoparticles. The immunoelectrode displays very good electrochemical properties towards detection of IgG via using double-encoded magnetic redox-active nanoparticles as trace and hydrogen peroxide as enzyme substrate. Its limit of detection (10 pmol.L -1 ) is 10-fold better than that of using plain POx-anti-IgG secondary antibodies. The method was applied to the detection of IgG in serum samples, and an excellent correspondence with the reference values was found. (author)

High-energy particle emission from galena and pyrite bombarded with Cs and O ions

International Nuclear Information System (INIS)

Karpuzov, D.S.; McIntyre, N.S.

2002-01-01

The ejection of energetic particles during steady-state ion surface bombardment has been investigated by means of a dynamic computer simulation as well as in a secondary ion mass spectrometry (SIMS)/low-energy ion scattering from surfaces (LEIS) experiment. The emphasis of this comparative study is on the mass dependence of high-energy tails in sputtering and backscattering for the bombardment of galena (PbS) and pyrite (FeS 2 ) with keV energy ion beam of cesium and oxygen. In the experiment, kinetic energy distributions of sputtered secondary ions (S + , Fe + , Pb + , S - ), as well as backscattered or re-sputtered primary ions (Cs + , O + , O - ), have been measured on a modified Cameca IMS-3f magnetic sector mass spectrometer for keV cesium (Cs + ) and oxygen (O 2 + , O - ) bombardment of galena and pyrite. Ejection of high-energy particles, with emission energies of up to ∼40% or up to ∼60% of the bombarding energy for sputtering of the lighter component (S ± ) with cesium or oxygen, respectively, and of up to ∼40% (Cs + ) and ∼80% (O ± ) for backscattering, has been observed for PbS. The computer simulations were based on the well-known MARLOWE code. In order to model the change of the stoichiometry of the binary compounds, dynamic modification of the target composition in the near-surface region was introduced. Cs incorporation was included, and a relative enrichment of the metallic component (Pb, Fe) in the top few layers due to preferential sputtering of sulfur was allowed. The computer simulations provide information on the formation of altered layer under sputter equilibrium as well as on the energy and angular emission distributions of sputtered and backscattered particles in steady-state conditions. Multiple scattering of Cs projectiles and dynamic re-sputtering of cesium that was previously incorporated in the altered near-surface region can be distinguished in the simulation, and matched with the experimental observations. In addition

Photon emission produced by particle-surface collisions

International Nuclear Information System (INIS)

White, C.W.; Tolk, N.H.

1976-02-01

Visible, ultraviolet, and infrared optical emission results from low-energy (20 eV-10 keV) particle-surface collisions. Several distinct kinds of collision induced optical radiation are discussed which provide fundamental information on particle-solid collision processes. Line radiation arises from excited states of sputtered surface constituents and backscattered beam particles. This radiation uniquely identifies the quantum state of sputtered or reflected particles, provides a method for identifying neutral atoms sputtered from the surface, and serves as the basis for a sensitive surface analysis technique. Broadband radiation from the bulk of the solid is attributed to the transfer of projectile energy to the electrons in the solid. Continuum emission observed well in front of transition metal targets is believed to arise from excited atom clusters (diatomic, triatomic, etc.) ejected from the solid in the sputtering process. Application of sputtered atom optical radiation for surface and depth profile analysis is demonstrated for the case of submonolayer quantities of chromium on silicon and aluminum implanted in SiO 2

Depth distribution of damage obtained by Rutherford backscattering combined with channeling

International Nuclear Information System (INIS)

Behrisch, R.; Roth, J.

1976-01-01

The different approaches to determine depth distributions of damage in solids by Rutherford backscattering combined with channeling are reviewed. These methods are best applicable for damage introduced by ion bombardment. Most investigations up to now have been done at semiconductors where the ion damage seems to be more suited for analysis by this method than the ion damage in metals. The quantity used for getting depth profiles is mostly the increase in minimum yields in single alignment Rutherford backscattering, while only few measurements have been done at double alignment and at slight misalignment, i.e., the sides of the channeling dips

Shallow water acoustic backscatter and reverberation measurements using a 68-kHz cylindrical array

Science.gov (United States)

Gallaudet, Timothy Cole

2001-10-01

The characterization of high frequency, shallow water acoustic backscatter and reverberation is important because acoustic systems are used in many scientific, commercial, and military applications. The approach taken is to use data collected by the Toroidal Volume Search Sonar (TVSS), a 68 kHz multibeam sonar capable of 360° imaging in a vertical plane perpendicular to its direction of travel. With this unique capability, acoustic backscatter imagery of the seafloor, sea surface, and horizontal and vertical planes in the volume are constructed from data obtained in 200m deep waters in the Northeastern Gulf of Mexico when the TVSS was towed 78m below the surface, 735m astern of a towship. The processed imagery provide a quasi-synoptic characterization of the spatial and temporal structure of boundary and volume acoustic backscatter and reverberation. Diffraction, element patterns, and high sidelobe levels are shown to be the most serious problems affecting cylindrical arrays such as the TVSS, and an amplitude shading method is presented for reducing the peak sidelobe levels of irregular-line and non-coplanar arrays. Errors in the towfish's attitude and motion sensor, and irregularities in the TVSS's transmitted beampattern produce artifacts in the TVSS-derived bathymetry and seafloor acoustic backscatter imagery. Correction strategies for these problems are described, which are unique in that they use environmental information extracted from both ocean boundaries. Sea surface and volume acoustic backscatter imagery are used to explore and characterize the structure of near-surface bubble clouds, schooling fish, and zooplankton. The simultaneous horizontal and vertical coverage provided by the TVSS is shown to be a primary advantage, motivating further use of multibeam sonars in these applications. Whereas boundary backscatter fluctuations are well described by Weibull, K, and Rayleigh mixture probability distributions, those corresponding to volume backscatter are

Correlations between calcineurin phosphatase inhibition and cyclosporine metabolites concentrations in kidney transplant recipients: Implications for immunoassays

DEFF Research Database (Denmark)

Jørgensen, Kaj Anker; Karamperis, Nikolaos; Koefoed-Nielsen, Pernille Bundgaard

2006-01-01

by inhibiting the enzyme calcineurin phosphatase. Determination of the enzyme's activity is one of the most promising pharmacodynamic markers. It is unknown how calcineurin phosphatase inhibition correlates with various cyclosporine monitoring assays and what is the potential impact of metabolites...... by the enzyme multiplied immunoassay technique (EMIT) and by the polyclonal fluorescence polarization immunoassay (pFPIA). Calcineurin phosphatase activity was measured by its ability to dephosphorylate a previously phosphorylated 19-amino acid peptide. We found that calcineurin phosphatase inhibition...

Correlations between calcineurin phosphatase inhibition and cyclosporine metabolites concentrations in kidney transplant recipients: implications for immunoassays

DEFF Research Database (Denmark)

Karamperis, N; Koefoed-Nielsen, PB; Brahe, P

2006-01-01

by inhibiting the enzyme calcineurin phosphatase. Determination of the enzyme's activity is one of the most promising pharmacodynamic markers. It is unknown how calcineurin phosphatase inhibition correlates with various cyclosporine monitoring assays and what is the potential impact of metabolites...... by the enzyme multiplied immunoassay technique (EMIT) and by the polyclonal fluorescence polarization immunoassay (pFPIA). Calcineurin phosphatase activity was measured by its ability to dephosphorylate a previously phosphorylated 19-amino acid peptide. We found that calcineurin phosphatase inhibition...

Analytic representation of the backscatter correction factor at the exit of high energy photon beams

International Nuclear Information System (INIS)

Kappas, K.; Rosenwald, J.C.

1991-01-01

In high-energy X-ray beams, the dose calculated near the exit surface under electronic equilibrium conditions is generally over-estimated since it is derived from measurements performed in water with large thickness of backscattering material. The resulting error depends on a number of parameters such as beam energy, field dimension, thickness of overlying and underlying material. The authors have systematically measured for 4 different energies and for different para- meters and for different combinations of the above parameters, the reduction of dose due to backscatter. This correction is expressed as a multiplicative factor, called 'Backscatter Correction Factor' (BCF). This BCF is larger for lower energies, larger field sizes and larger depths. The BCF has been represented by an analytical expression which involves an exponential function of the backscattering thickness and linear relationships with depth field size and beam quality index. Using this expression, the BCF can be calculated within 0.5% for any conditions in the energy range investigated. (author). 14 refs.; 4 figs.; 3 tabs

Multiplex dipstick immunoassay for semi-quantitative determination of Fusarium mycotoxins in cereals

Energy Technology Data Exchange (ETDEWEB)

Lattanzio, Veronica M.T., E-mail: veronica.lattanzio@ispa.cnr.it [National Research Council of Italy, Institute of Sciences of Food Production (ISPA-CNR), Via Amendola 122/O, 70126 Bari (Italy); Nivarlet, Noan [UNISENSOR S.A., Zoning industriel du Dossay, Rue du Dossay no 3, B-4020 Liege (Belgium); Lippolis, Vincenzo; Gatta, Stefania Della [National Research Council of Italy, Institute of Sciences of Food Production (ISPA-CNR), Via Amendola 122/O, 70126 Bari (Italy); Huet, Anne-Catherine; Delahaut, Philippe [Centre d' Economie Rurale (CER Groupe), Rue du Point du Jour no 8, B-6900 Marloie (Belgium); Granier, Benoit [UNISENSOR S.A., Zoning industriel du Dossay, Rue du Dossay no 3, B-4020 Liege (Belgium); Visconti, Angelo [National Research Council of Italy, Institute of Sciences of Food Production (ISPA-CNR), Via Amendola 122/O, 70126 Bari (Italy)

2012-03-09

Highlights: Black-Right-Pointing-Pointer We developed a rapid method based on a multiplex dipstick immunoassay. Black-Right-Pointing-Pointer The assay allowed the determination of major Fusarium toxins in wheat, oats, maize. Black-Right-Pointing-Pointer We obtained cut off levels close to EU regulatory levels. - Abstract: A multiplex dipstick immunoassay based method for the simultaneous determination of major Fusarium toxins, namely zearalenone, T-2 and HT-2 toxins, deoxynivalenol and fumonisins in wheat, oats and maize has been developed. The dipstick format was based on an indirect competitive approach. Four test lines (mycotoxin-BSA conjugates) and one control line were located on the strip membrane. Labelled antibodies were freeze-dried within the microwell. Two matrix-related sample preparation protocols have been developed for wheat/oats (not containing fumonisins) and maize (containing fumonisins) respectively. The use of a methanol/water mixture for sample preparation allowed recoveries in the range 73-109% for all mycotoxins in all tested cereals, with relative standard deviation less than 10%. The optimized immunoassay was able to detect target mycotoxins at cut off levels equal to 80% of EU maximum permitted levels, i.e. 280, 400, 1400 and 3200 {mu}g kg{sup -1}, respectively, for zearalenone, T-2/HT-2 toxins, deoxynivalenol and fumonisins in maize, and 80, 400 and 1400 {mu}g kg{sup -1}, respectively, for zearalenone, T-2/HT-2 toxins and deoxynivalenol in wheat and oats. Analysis of naturally contaminated samples resulted in a good agreement between multiplex dipstick and validated confirmatory LC-MS/MS. The percentage of false positive results was less than or equal to 13%, whereas no false negative results were obtained. Data on the presence/absence of 6 mycotoxins at levels close to EU regulatory levels were obtained within 30 min. The proposed immunoassay protocol is rapid, inexpensive, easy-to-use and fit for purpose of rapid screening of mycotoxins

Aequorin fusion proteins as bioluminescent tracers for competitive immunoassays

Science.gov (United States)

Mirasoli, Mara; Michelini, Elisa; Deo, Sapna K.; Dikici, Emre; Roda, Aldo; Daunert, Sylvia

2004-06-01

The use of bio- and chemiluminescence for the development of quantitative binding assays offers undoubted advantages over other detection systems, such as spectrophotometry, fluorescence, or radioactivity. Indeed, bio- and chemiluminescence detection provides similar, or even better, sensitivity and detectability than radioisotopes, while avoiding the problems of health hazards, waste disposal, and instability associated with the use of radioisotopes. Among bioluminescent labels, the calcium-activated photoprotein aequorin, originally isolated from Aequorea victoria and today available as a recombinant product, is characterized by very high detectability, down to attomole levels. It has been used as a bioluminescent label for developing a variety of highly sensitive immunoassays, using various analyte-aequorin conjugation strategies. When the analyte is a protein or a peptide, genetic engineering techniques can be used to produce protein fusions where the analyte is in-frame fused with aequorin, thus producing homogeneous one-to-one conjugation products, available in virtually unlimited amount. Various assays were developed using this strategy: a short review of the most interesting applications is presented, as well as the cloning, purification and initial characterization of an endothelin-1-aequorin conjugate suitable for developing a competitive immunoassay for endothelin-1, a potent vasoconstrictor peptide, involved in hypertension.

Detection of alpha-fetoprotein in magnetic immunoassay of thin channels using biofunctional nanoparticles

Science.gov (United States)

Tsai, H. Y.; Gao, B. Z.; Yang, S. F.; Li, C. S.; Fuh, C. Bor

2014-01-01

This paper presents the use of fluorescent biofunctional nanoparticles (10-30 nm) to detect alpha-fetoprotein (AFP) in a thin-channel magnetic immunoassay. We used an AFP model biomarker and s-shaped deposition zones to test the proposed detection method. The results show that the detection using fluorescent biofunctional nanoparticle has a higher throughput than that of functional microparticle used in previous experiments on affinity reactions. The proposed method takes about 3 min (versus 150 min of previous method) to detect 100 samples. The proposed method is useful for screening biomarkers in clinical applications, and can reduce the run time for sandwich immunoassays to less than 20 min. The detection limits (0.06 pg/ml) and linear ranges (0.068 pg/ml-0.68 ng/ml) of AFP using fluorescent biofunctional nanoparticles are the same as those of using functional microparticles within experimental errors. This detection limit is substantially lower and the linear range is considerably wider than those of enzyme-linked immunosorbent assay (ELISA) and other methods in sandwich immunoassay methods. The differences between this method and an ELISA in AFP measurements of serum samples were less than 12 %. The proposed method provides simple, fast, and sensitive detection with a high throughput for biomarkers.

Trace-level mercury ion (Hg2+) analysis in aqueous sample based on solid-phase extraction followed by microfluidic immunoassay.

Science.gov (United States)

Date, Yasumoto; Aota, Arata; Terakado, Shingo; Sasaki, Kazuhiro; Matsumoto, Norio; Watanabe, Yoshitomo; Matsue, Tomokazu; Ohmura, Naoya

2013-01-02

Mercury is considered the most important heavy-metal pollutant, because of the likelihood of bioaccumulation and toxicity. Monitoring widespread ionic mercury (Hg(2+)) contamination requires high-throughput and cost-effective methods to screen large numbers of environmental samples. In this study, we developed a simple and sensitive analysis for Hg(2+) in environmental aqueous samples by combining a microfluidic immunoassay and solid-phase extraction (SPE). Using a microfluidic platform, an ultrasensitive Hg(2+) immunoassay, which yields results within only 10 min and with a lower detection limit (LOD) of 0.13 μg/L, was developed. To allow application of the developed immunoassay to actual environmental aqueous samples, we developed an ion-exchange resin (IER)-based SPE for selective Hg(2+) extraction from an ion mixture. When using optimized SPE conditions, followed by the microfluidic immunoassay, the LOD of the assay was 0.83 μg/L, which satisfied the guideline values for drinking water suggested by the United States Environmental Protection Agency (USEPA) (2 μg/L; total mercury), and the World Health Organisation (WHO) (6 μg/L; inorganic mercury). Actual water samples, including tap water, mineral water, and river water, which had been spiked with trace levels of Hg(2+), were well-analyzed by SPE, followed by microfluidic Hg(2+) immunoassay, and the results agreed with those obtained from reduction vaporizing-atomic adsorption spectroscopy.

Using multi-beam echo sounder backscatter data for sediment classification in very shallow water environments

NARCIS (Netherlands)

Amiri-Simkooei, A.R.; Snellen, M.; Simons, D.G.

2009-01-01

In a recent work described in Ref. [1], an angle-independent methodology was developed to use the multi-beam echo sounder backscatter (MBES) data for the seabed sediment classification. The method employs the backscatter data at a certain angle to obtain the number of sediment classes and to

High-resolution backscatter power observations of 440-MHz E region coherent echoes at Millstone Hill

International Nuclear Information System (INIS)

Foster, J.C.; Tetenbaum, D.

1991-01-01

A 40-μs pulse length has been used to provide 10-s temporal and 6-km range resolution observations of E region coherent backscatter from the premidnight eastward electrojet region to the north of Millstone Hill. The observations can be divided into two categories: strong events in which the backscattered amplitude nears saturation and weak events in which spatial structure and large-amplitude variations are common. Calibrated observations find a typical volume scattering coefficient of ∼10 -11 m -1 at 440 MHz during strong events with a maximum level of 9 x 10 -10 m -1 observed for brief intervals. During less intense events the radar backscatter is modulated by ∼30dB in amplitude at Pc 5 frequencies (150-500 s) by waves with spatial wavelength 50-100 km. The observations support the premise that the weak irregularities grow linearly with electric field strength and reach a saturation amplitude beyond which the oscillating electric field of the Pc pulsation has little effect. The observed variation of backscattered power with range is interpreted using a geometrical model which accounts for the detailed antenna beam pattern, a magnetic aspect angle sensitivity of -10 dB per degree, and a thin layer of irregularities centered at 110 km altitude. For strongly driven conditions a comparison of the range variation of backscattered power with the thin layer model suggests that the signal power becomes increasingly dominated by strong scatters confined to a narrower altitude range. The apparent altitude extent of the strongest irregularities decreases by a factor of 2 as the amplitude of the backscattered signal increases by a factor of 10

Corneal backscatter in insulin-dependent and non-insulin-dependent diabetes mellitus patients: a pilot study

Directory of Open Access Journals (Sweden)

Ana M Calvo-Maroto

Full Text Available ABSTRACT Purpose: To compare central corneal backscatter obtained from Scheimpflug images between patients with insulin-dependent and non-insulin-dependent diabetes mellitus (IDDM and NIDDM, respectively and healthy controls. Methods: Seven patients with IDDM (7 eyes, eleven patients with NIDDM (11 eyes, and sixteen healthy subjects (16 eyes were included in this pilot study. Scheimpflug imaging system (Pentacam, Oculus Inc., Germany was used to obtain optical sections of the cornea. Seven meridians were analyzed for each eye, oriented from 70° to 110°. Optical density values for the central 3-mm and 5-mm zones of the cornea were obtained by image analysis using external software. Results: Corneal backscatter was significantly higher in the diabetic patients than in the controls for the central 3-mm (p=0.016 and 5-mm (p=0.014 zones. No significant differences in corneal backscatter were found between the IDDM and NIDDM groups for either zone (both p>0.05. In the NIDDM group, significant correlations were observed for both central zones between corneal backscatter and age (3 mm: r=0.604, p=0.025; 5 mm: r=0.614, p=0.022 and central corneal thickness (3 mm: r=0.641, p=0.017; 5 mm: r=0.671, p=0.012; this was not found in the IDDM group (p>0.05. The presence of diabetes showed a significant effect on central corneal backscatter (Kruskal-Wallis test, p<0.001. Conclusions: Diabetic patients showed higher values of corneal light backscatter than healthy subjects. Corneal optical density analysis may be a useful tool for monitoring and assessing the ocular changes caused by diabetes.

Ultrasonic device for real-time sewage velocity and suspended particles concentration measurements.

Science.gov (United States)

Abda, F; Azbaid, A; Ensminger, D; Fischer, S; François, P; Schmitt, P; Pallarès, A

2009-01-01

In the frame of a technological research and innovation network in water and environment technologies (RITEAU, Réseau de Recherche et d'Innovation Technologique Eau et Environnement), our research group, in collaboration with industrial partners and other research institutions, has been in charge of the development of a suitable flowmeter: an ultrasonic device measuring simultaneously the water flow and the concentration of size classes of suspended particles. Working on the pulsed ultrasound principle, our multi-frequency device (1 to 14 MHz) allows flow velocity and water height measurement and estimation of suspended solids concentration. Velocity measurements rely on the coherent Doppler principle. A self developed frequency estimator, so called Spectral Identification method, was used and compared to the classical Pulse-Pair method. Several measurements campaigns on one wastewater collector of the French city of Strasbourg gave very satisfactory results and showed smaller standard deviation values for the Doppler frequency extracted by the Spectral Identification method. A specific algorithm was also developed for the water height measurements. It relies on the water surface acoustic impedance rupture and its peak localisation and behaviour in the collected backscattering data. This algorithm was positively tested on long time measurements on the same wastewater collector. A large part of the article is devoted to the measurements of the suspended solids concentrations. Our data analysis consists in the adaptation of the well described acoustic behaviour of sand to the behaviour of wastewater particles. Both acoustic attenuation and acoustic backscattering data over multiple frequencies are analyzed for the extrapolation of size classes and respective concentrations. Under dry weather conditions, the massic backscattering coefficient and the overall size distribution showed similar evolution whatever the measurement site was and were suggesting a global

X-ray backscatter imaging for radiography by selective detection and snapshot: Evolution, development, and optimization

Science.gov (United States)

Shedlock, Daniel

Compton backscatter imaging (CBI) is a single-sided imaging technique that uses the penetrating power of radiation and unique interaction properties of radiation with matter to image subsurface features. CBI has a variety of applications that include non-destructive interrogation, medical imaging, security and military applications. Radiography by selective detection (RSD), lateral migration radiography (LMR) and shadow aperture backscatter radiography (SABR) are different CBI techniques that are being optimized and developed. Radiography by selective detection (RSD) is a pencil beam Compton backscatter imaging technique that falls between highly collimated and uncollimated techniques. Radiography by selective detection uses a combination of single- and multiple-scatter photons from a projected area below a collimation plane to generate an image. As a result, the image has a combination of first- and multiple-scatter components. RSD techniques offer greater subsurface resolution than uncollimated techniques, at speeds at least an order of magnitude faster than highly collimated techniques. RSD scanning systems have evolved from a prototype into near market-ready scanning devices for use in a variety of single-sided imaging applications. The design has changed to incorporate state-of-the-art detectors and electronics optimized for backscatter imaging with an emphasis on versatility, efficiency and speed. The RSD system has become more stable, about 4 times faster, and 60% lighter while maintaining or improving image quality and contrast over the past 3 years. A new snapshot backscatter radiography (SBR) CBI technique, shadow aperture backscatter radiography (SABR), has been developed from concept and proof-of-principle to a functional laboratory prototype. SABR radiography uses digital detection media and shaded aperture configurations to generate near-surface Compton backscatter images without scanning, similar to how transmission radiographs are taken. Finally, a

An enzyme immunoassay to quantify neurofilament light chain in cerebrospinal fluid.

NARCIS (Netherlands)

Geel, W.J.A. van; Rosengren, L.E.; Verbeek, M.M.

2005-01-01

Neurofilament light chain is a component of the axonal cytoskeleton. The concentration of the neurofilament light chain in cerebrospinal fluid may reflect axonal damage or the extent of white matter damage. In this study we describe a sensitive immunoassay for the detection of neurofilament light

IMMUNOASSAY METHOD FOR THE DETERMINATION OF PENTACHLOROPHENOL IN SOIL AND SEDIMENT

Science.gov (United States)

The journal article describes the use of a prototype immunoassay method for the determination of pentacholorphenol (PCP) in soil and sediment. PCP was used as a pesticide and wood preservative and is not currently available to the general public. The paper stresses the importan...

A three-dimensional, two-way, parabolic equation model for acoustic backscattering in a cylindrical coordinate system

DEFF Research Database (Denmark)

Zhu, Dong; Jensen, Leif Bjørnø

2000-01-01

. The major drawback of using the cylindrical coordinate system, when the backscattering solution is valid within a limited area, is analyzed using a geometrical-optical interpretation. The model may be useful for studying three-dimensional backscattering phenomena comprising azimuthal diffraction effects...

Electromagnetic backscattering from freak waves in (1 + 1)-dimensional deep-water

International Nuclear Information System (INIS)

Tao, Xie; Tao, Shen; Wei, Chen; Hai-Lan, Kuang; Perrie, William

2010-01-01

To study the electromagnetic (EM) backscatter characteristics of freak waves at moderate incidence angles, we establish an EM backscattering model for freak waves in (1 + 1)-dimensional deep water. The nonlinear interaction between freak waves and Bragg short waves is considered to be the basic hydrodynamic spectra modulation mechanism in the model. Numerical results suggest that the EM backscattering intensities of freak waves are less than those from the background sea surface at moderate incidence angles. The normalised radar cross sections (NRCSs) from freak waves are highly polarisation dependent, even at low incidence angles, which is different from the situation for normal sea waves; moreover, the NRCS of freak waves is more polarisation dependent than the background sea surface. NRCS discrepancies between freak waves and the background sea surface with using horizontal transmitting horizomtal (HH) polarisation are larger than those using vertical transmitting vertical (VV) polarisation, at moderate incident angles. NRCS discrepancies between freak waves and background sea surface decreases with the increase of incidence angle, in both HH and VV polarisation radars. As an application, in the synthetic-aperture radar (SAR) imaging of freak waves, we suggest that freak waves should have extremely low backscatter NRCSs for the freak wave facet with the strongest slope. Compared with the background sea surface, the freak waves should be darker in HH polarisation echo images than in VV echo images, in SAR images. Freak waves can be more easily detected from the background sea surface in HH polarisation images than in VV polarisation images. The possibility of detection of freak waves at low incidence angles is much higher than at high incidence angles. (classical areas of phenomenology)

Near-surface fault detection by migrating back-scattered surface waves with and without velocity profiles

KAUST Repository

Yu, Han

2016-04-26

We demonstrate that diffraction stack migration can be used to discover the distribution of near-surface faults. The methodology is based on the assumption that near-surface faults generate detectable back-scattered surface waves from impinging surface waves. We first isolate the back-scattered surface waves by muting or FK filtering, and then migrate them by diffraction migration using the surface wave velocity as the migration velocity. Instead of summing events along trial quasi-hyperbolas, surface wave migration sums events along trial quasi-linear trajectories that correspond to the moveout of back-scattered surface waves. We have also proposed a natural migration method that utilizes the intrinsic traveltime property of the direct and the back-scattered waves at faults. For the synthetic data sets and the land data collected in Aqaba, where surface wave velocity has unexpected perturbations, we migrate the back-scattered surface waves with both predicted velocity profiles and natural Green\\'s function without velocity information. Because the latter approach avoids the need for an accurate velocity model in event summation, both the prestack and stacked migration images show competitive quality. Results with both synthetic data and field records validate the feasibility of this method. We believe applying this method to global or passive seismic data can open new opportunities in unveiling tectonic features.

Immunoassay for thymopoietin

International Nuclear Information System (INIS)

Goldstein, G.

1979-01-01

The patent describes the development of a radio-immunoassay for thymopoietin in biological samples. The method of raising antibodies to this polypeptide hormone is described. This is achieved by injecting a host animal with an antigen consisting of thymopoietin covalently bonded by glutaraldehyde to a carrier protein such as bovine serum albumin and equine globulin. Different methods of radiolabelling thymopoietin with 125 I for use as the tracer antigen are described. The Bolton-Hunter procedure was preferred to the chloramine-T method since direct iodination of the tyrosyl moieties of thymopoietin resulted in some loss of immunoreactivity. Systems for separating the antigen-antibody complex and unbound antigen are compared. Binding-inhibition curves for unlabelled thymopoietin in the assay employing polyethylene glycol separation showed a sensitivity of 5 ng thymopoietin/ml. However, using the double antibody or dextran coated charcoal separation techniques, the sensitivity of thymopoietin was 0.1 ng/ml. Thus these latter two procedures are thus especially suitable for measuring thymopoietin levels in serum or plasma samples. The assay was shown to be specific for thymopoietin, no significant displacement being produced by control polypeptides. (U.K.)

IMPROVED PARAMETERIZATION OF WATER CLOUD MODEL FOR HYBRID-POLARIZED BACKSCATTER SIMULATION USING INTERACTION FACTOR

Directory of Open Access Journals (Sweden)

S. Chauhan

2017-07-01

Full Text Available The prime aim of this study was to assess the potential of semi-empirical water cloud model (WCM in simulating hybrid-polarized SAR backscatter signatures (RH and RV retrieved from RISAT-1 data and integrate the results into a graphical user interface (GUI to facilitate easy comprehension and interpretation. A predominant agricultural wheat growing area was selected in Mathura and Bharatpur districts located in the Indian states of Uttar Pradesh and Rajasthan respectively to carry out the study. The three-date datasets were acquired covering the crucial growth stages of the wheat crop. In synchrony, the fieldwork was organized to measure crop/soil parameters. The RH and RV backscattering coefficient images were extracted from the SAR data for all the three dates. The effect of four combinations of vegetation descriptors (V1 and V2 viz., LAI-LAI, LAI-Plant water content (PWC, Leaf water area index (LWAI-LWAI, and LAI-Interaction factor (IF on the total RH and RV backscatter was analyzed. The results revealed that WCM calibrated with LAI and IF as the two vegetation descriptors simulated the total RH and RV backscatter values with highest R2 of 0.90 and 0.85 while the RMSE was lowest among the other tested models (1.18 and 1.25 dB, respectively. The theoretical considerations and interpretations have been discussed and examined in the paper. The novelty of this work emanates from the fact that it is a first step towards the modeling of hybrid-polarized backscatter data using an accurately parameterized semi-empirical approach.

Direct detection of near-surface faults by migration of back-scattered surface waves

KAUST Repository

Yu, Han

2014-08-05

We show that diffraction stack migration can be used to estimate the distribution of near-surface faults. The assumption is that near-surface faults generate detectable back-scattered surface waves from impinging surface waves. The processing steps are to isolate the back-scattered surface waves, and then migrate them by diffraction migration using the surface wave velocity as the migration velocity. Instead of summing events along trial quasi-hyperbolas, surface wave migration sums events along trial quasi-linear trajectories that correspond to the moveout of back-scattered surface waves. A deconvolution filter derived from the data can be used to collapse a dispersive arrival into a non-dispersive event. Results with synthetic data and field records validate the feasibility of this method. Applying this method to USArray data or passively recorded exploration data might open new opportunities in mapping tectonic features over the extent of the array.

Reson 8101 Backscatter imagery of Penguin Bank, Molokai, Hawaii, USA

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Backscatter imagery extracted from gridded bathymetry of Penguin Bank, Molokai, Hawaii, USA. These data provide almost complete coverage between 0 and 100 meters....

Serum sample containing endogenous antibodies interfering with multiple hormone immunoassays. Laboratory strategies to detect interference

Directory of Open Access Journals (Sweden)

Elena García-González

2016-04-01

Full Text Available Objectives: Endogenous antibodies (EA may interfere with immunoassays, causing erroneous results for hormone analyses. As (in most cases this interference arises from the assay format and most immunoassays, even from different manufacturers, are constructed in a similar way, it is possible for a single type of EA to interfere with different immunoassays. Here we describe the case of a patient whose serum sample contains EA that interfere several hormones tests. We also discuss the strategies deployed to detect interference. Subjects and methods: Over a period of four years, a 30-year-old man was subjected to a plethora of laboratory and imaging diagnostic procedures as a consequence of elevated hormone results, mainly of pituitary origin, which did not correlate with the overall clinical picture. Results: Once analytical interference was suspected, the best laboratory approaches to investigate it were sample reanalysis on an alternative platform and sample incubation with antibody blocking tubes. Construction of an in-house ‘nonsense’ sandwich assay was also a valuable strategy to confirm interference. In contrast, serial sample dilutions were of no value in our case, while polyethylene glycol (PEG precipitation gave inconclusive results, probably due to the use of inappropriate PEG concentrations for several of the tests assayed. Conclusions: Clinicians and laboratorians must be aware of the drawbacks of immunometric assays, and alert to the possibility of EA interference when results do not fit the clinical pattern. Keywords: Endogenous antibodies, Immunoassay, Interference, Pituitary hormones, Case report

Investigations of multiple backscattering and albedos of 1.12 MeV gamma photons in elements and alloys

International Nuclear Information System (INIS)

Sabharwal, Arvind D.; Singh, Bhajan; Sandhu, B.S.

2009-01-01

The energy and intensity distributions of multiple backscattering of 1.12 MeV gamma photons emerging from targets of elements and alloys are observed as a function of thickness and atomic number (Z) of the target. The numbers of these multiply backscattered events show an increase with increase in target thickness, and then saturate for a particular thickness of the target called saturation thickness (depth). The saturation thickness decreases with increasing atomic number and varies as e -Z . The multiple backscattering, an interfering background noise in Compton profile, has been successfully used to assign the 'effective atomic number' to alloys. Monte Carlo calculations also support the present experimental results. The number, energy and dose albedos are also found to be saturating for the same thickness where the numbers of multiply backscattered events saturate.

Detection of Avian Influenza Virus by Fluorescent DNA Barcode-based Immunoassay with Sensitivity Comparable to PCR

DEFF Research Database (Denmark)

Cao, Cuong; Dhumpa, Raghuram; Bang, Dang Duong

2010-01-01

involves the sandwiching of the target AIV between magnetic immunoprobes and barcode-carrying immunoprobes. Because each barcode-carrying immunoprobe is functionalized with a multitude of fluorophore-DNA barcode strands, many DNA barcodes are released for each positive binding event resulting......In this paper, a coupling of fluorophore-DNA barcode and bead-based immunoassay for detecting avian influenza virus (AIV) with PCR-like sensitivity is reported. The assay is based on the use of sandwich immunoassay and fluorophore-tagged oligonucleotides as representative barcodes. The detection...

Monte Carlo simulation of MOSFET dosimeter for electron backscatter using the GEANT4 code.

Science.gov (United States)

Chow, James C L; Leung, Michael K K

2008-06-01

The aim of this study is to investigate the influence of the body of the metal-oxide-semiconductor field effect transistor (MOSFET) dosimeter in measuring the electron backscatter from lead. The electron backscatter factor (EBF), which is defined as the ratio of dose at the tissue-lead interface to the dose at the same point without the presence of backscatter, was calculated by the Monte Carlo simulation using the GEANT4 code. Electron beams with energies of 4, 6, 9, and 12 MeV were used in the simulation. It was found that in the presence of the MOSFET body, the EBFs were underestimated by about 2%-0.9% for electron beam energies of 4-12 MeV, respectively. The trend of the decrease of EBF with an increase of electron energy can be explained by the small MOSFET dosimeter, mainly made of epoxy and silicon, not only attenuated the electron fluence of the electron beam from upstream, but also the electron backscatter generated by the lead underneath the dosimeter. However, this variation of the EBF underestimation is within the same order of the statistical uncertainties as the Monte Carlo simulations, which ranged from 1.3% to 0.8% for the electron energies of 4-12 MeV, due to the small dosimetric volume. Such small EBF deviation is therefore insignificant when the uncertainty of the Monte Carlo simulation is taken into account. Corresponding measurements were carried out and uncertainties compared to Monte Carlo results were within +/- 2%. Spectra of energy deposited by the backscattered electrons in dosimetric volumes with and without the lead and MOSFET were determined by Monte Carlo simulations. It was found that in both cases, when the MOSFET body is either present or absent in the simulation, deviations of electron energy spectra with and without the lead decrease with an increase of the electron beam energy. Moreover, the softer spectrum of the backscattered electron when lead is present can result in a reduction of the MOSFET response due to stronger

The Backscattering Phase Function for a Sphere with a Two-Scale Relief of Rough Surface

Science.gov (United States)

Klass, E. V.

2017-12-01

The backscattering of light from spherical surfaces characterized by one and two-scale roughness reliefs has been investigated. The analysis is performed using the three-dimensional Monte-Carlo program POKS-RG (geometrical-optics approximation), which makes it possible to take into account the roughness of objects under study by introducing local geometries of different levels. The geometric module of the program is aimed at describing objects by equations of second-order surfaces. One-scale roughness is set as an ensemble of geometric figures (convex or concave halves of ellipsoids or cones). The two-scale roughness is modeled by convex halves of ellipsoids, with surface containing ellipsoidal pores. It is shown that a spherical surface with one-scale convex inhomogeneities has a flatter backscattering phase function than a surface with concave inhomogeneities (pores). For a sphere with two-scale roughness, the dependence of the backscattering intensity is found to be determined mostly by the lower-level inhomogeneities. The influence of roughness on the dependence of the backscattering from different spatial regions of spherical surface is analyzed.

On-Chip Immunoassay for Determination of Urinary Albumin

OpenAIRE

Laiwattanapaisal, Wanida; Songjaroen, Temsiri; Maturos, Thitima; Lomas, Tanom; Sappat, Assawapong; Tuantranont, Adisorn

2009-01-01

An immunoassay performed on a portable microfluidic device was evaluated for the determination of urinary albumin. An increase in absorbance at 500 nm resulting from immunoagglutination was monitored directly on the poly(dimethylsiloxane) (PDMS) microchip using a portable miniature fibre-optic spectrometer. A calibration curve was linear up to 10 mg L–1 (r2 = 0.993), with a detection limit of 0.81 mg L–1 (S/N = 3). The proposed system showed good precision, with relative standard deviations (...

Numerical study of bandwidth effect on stimulated Raman backscattering in nonlinear regime

Science.gov (United States)

Zhou, H. Y.; Xiao, C. Z.; Zou, D. B.; Li, X. Z.; Yin, Y.; Shao, F. Q.; Zhuo, H. B.

2018-06-01

Nonlinear behaviors of stimulated Raman scattering driven by finite bandwidth pumps are studied by one dimensional particle-in-cell simulations. The broad spectral feature of plasma waves and backscattered light reveals the different coupling and growth mechanisms, which lead to the suppression effect before the deep nonlinear stage. It causes nonperiodic plasma wave packets and reduces packet and etching velocities. Based on the negative frequency shift and electron energy distribution, the long-time evolution of instability can be divided into two stages by the relaxation time. It is a critical time after which the alleviation effects of nonlinear frequency shift and hot electrons are replaced by enhancement. Thus, the broadband pump suppresses instability at early time. However, it aggravates in the deep nonlinear stage by lifting the saturation level due to the coupling of the incident pump with each frequency shifted plasma wave. Our simulation results show that the nonlinear effects are valid in a bandwidth range from 2.25% to 3.0%, and the physics are similar within a nearby parameter space.

New applications of old processes in nondestructive testing - irradiation and backscatter methods

International Nuclear Information System (INIS)

Segebade, C.

1995-01-01

The application of two non-destructive test processes based on photon irradiation measurement is described. The photon backscatter process and the irradiation measurement were used in the technical field and in examining artificial articles. With the aid of the two beam absorption method, wall thicknesses on large liquid containers made of polyethylene and of steel were measured. The same process with a somewhat modified test rig was used in measuring pipe wall thickness on an antique musical instrument. The components made of turbine blade material were excited to X-ray fluorescence with a source of radio nuclides and analysed with a semiconductor detector. This is particularly advantageous for elements which cannot be determined or can only be determined with difficulty by 'conventional' methods (e.g.: yttrium, rhenium). Also the wall thickness measurement for large (diameter approx. 6 m) plastic pipes with the aid of gamma backscatter is described, as is humidity measurement in brick material. Finally, there is a report on wood profile measurement in a stringed instrument with the aid of gamma backscatter. (orig./HP) [de

Airborne detection of oceanic turbidity cell structure using depth-resolved laser-induced water Raman backscatter

Science.gov (United States)

Hoge, F. E.; Swift, R. N.

1983-01-01

Airborne laser-induced, depth-resolved water Raman backscatter is useful in the detection and mapping of water optical transmission variations. This test, together with other field experiments, has identified the need for additional field experiments to resolve the degree of the contribution to the depth-resolved, Raman-backscattered signal waveform that is due to (1) sea surface height or elevation probability density; (2) off-nadir laser beam angle relative to the mean sea surface; and (3) the Gelbstoff fluorescence background, and the analytical techniques required to remove it. When converted to along-track profiles, the waveforms obtained reveal cells of a decreased Raman backscatter superimposed on an overall trend of monotonically decreasing water column optical transmission.

Lattice location of O{sup 18} in ion implanted Fe crystals by Rutherford backscattering spectrometry, channeling and nuclear reaction analysis

Energy Technology Data Exchange (ETDEWEB)

Vairavel, Mathayan; Sundaravel, Balakrishnan, E-mail: bsundar@igcar.gov.in; Panigrahi, Binaykumar

2016-09-15

There are contradictory theoretical predictions of lattice location of oxygen interstitial atom at tetrahedral and octahedral interstices in bcc Fe. For validating these predictions, 300 keV O{sup 18} ions with fluence of 5 × 10{sup 15} ions/cm{sup 2} are implanted into bcc Fe single crystals at room temperature and annealed at 400 °C. The Rutherford backscattering spectrometry (RBS) and nuclear reaction analysis (NRA)/channeling measurements are carried out with 850 keV protons. The lattice location of implanted O{sup 18} is analysed using the α-particles yield from O{sup 18}(p,α)N{sup 15} nuclear reaction. The tilt angular scans of α-particle yield along 〈110〉 and 〈100〉 axial directions are performed at room temperature. Lattice location of O{sup 18} is found to be at tetrahedral interstitial site by comparing the experimental scan with simulated scans using FLUX7 software.

Immunoassay: Principles, development and potential applications in the applied plant sciences

Energy Technology Data Exchange (ETDEWEB)

Hofman, P J

1986-02-01

The article briefly discusses the general principles of, and the methods involved in, immunoassay, and their development. Emplasis is placed on radioimmunoassay (RIA) and to a lesser extent, enzyme-linked immunosorbent assay (ELISA). The practical applications, with special reference to the citrus and subtropical fruit industries are discussed.

Effect of the stimulated Brillouin backscattering on selffocusing threshold

International Nuclear Information System (INIS)

Rubenchik, A.M.; Shapiro, E.G.; Turitsyn, S.K.

1994-03-01

In many physical problems stimulated Brillouin scattering (SBS) and selffocusing are manifested simultaneously. We consider effect of the stimulated Brillouin backscattering (SBS) on self-focusing of laser radiation in plasmas. It was found that the self-focusing may be supressed substantionally by the SBS effect

An algorithm to determine backscattering ratio and single scattering albedo

Digital Repository Service at National Institute of Oceanography (India)

Suresh, T.; Desa, E.; Matondkar, S.G.P.; Mascarenhas, A.A.M.Q.; Nayak, S.R.; Naik, P.

Algorithms to determine the inherent optical properties of water, backscattering probability and single scattering albedo at 490 and 676 nm from the apparent optical property, remote sensing reflectance are presented here. The measured scattering...

Design of novel hybrid organic-inorganic nanostructured biomaterials for immunoassay applications

Energy Technology Data Exchange (ETDEWEB)

Andrade, G [Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, PO Box 486, 31270.901, Belo Horizonte, MG (Brazil); Barbosa-Stancioli, E F [Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, PO Box 486, 31270.901, Belo Horizonte, MG (Brazil); Piscitelli Mansur, A A [Department of Metallurgical and Materials Engineering, Biomaterials and Tissue Engineering Laboratory, Federal University of Minas Gerais, Belo Horizonte, MG (Brazil); Vasconcelos, W L [Department of Metallurgical and Materials Engineering, Biomaterials and Tissue Engineering Laboratory, Federal University of Minas Gerais, Belo Horizonte, MG (Brazil); Mansur, H S [Department of Metallurgical and Materials Engineering, Biomaterials and Tissue Engineering Laboratory, Federal University of Minas Gerais, Belo Horizonte, MG (Brazil)

2006-12-01

The purpose of this study was to develop novel hybrid organic-inorganic materials based on poly(vinyl alcohol) (PVA) polymer chemically crosslinked network to be tested as solid support on bovine herpesvirus immunoassay. Hybrids were synthesized by reacting PVA with three different alkoxysilanes modifying chemical groups: tetraethoxysilane (TEOS), 3-mercaptopropyltrimethoxysilane (MPTMS) and 3-glycidoxypropyltrimethoxysilane (GPTMS). PVA-derived hybrids were also modified by chemically crosslinking with glutaraldehyde (GA) during the synthesis reaction. In order to investigate the structure in the nanometer-scale, PVA-derived hybrids were characterized by using small-angle x-ray scattering synchrotron radiation (SAXS) and x-ray diffraction (XRD). PVA hybrids' chemical functionalities and their interaction with herpesviruses were also characterized by Fourier transform infrared spectroscopy (FTIR). The bioactivity assays were tested through enzyme linked immunosorbent assay (ELISA). SAXS results have indicated nano-ordered disperse domains for PVA hybrids with different x-ray scattering patterns for PVA polymer and PVA-derived hybrids. FTIR spectra have shown major vibration bands associated with organic-inorganic chemical groups present in the PVA, PVA-derived by silane modifier and PVA chemically crosslinked by GA. The immunoassay results have shown that PVA hybrids with chemically functionalized structures regulated to some extent the specific bioimmobilization of herpesvirus onto solid phase. We think that it is due to the overall balance of forces associated with van der Waals interaction, hydrophilic and hydrophobic forces and steric hindrance acting at the surface. PVA and PVA-derived hybrid materials were successfully produced with GA crosslinking in a nanometer-scale network. Also, such a PVA-based material could be advantageously used in immunoassays with enhanced specificity for diagnosis.

Design of novel hybrid organic-inorganic nanostructured biomaterials for immunoassay applications

Energy Technology Data Exchange (ETDEWEB)

Andrade, G [Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, PO Box 486, 31270.901, Belo Horizonte, MG (Brazil); Barbosa-Stancioli, E F [Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, PO Box 486, 31270.901, Belo Horizonte, MG (Brazil); Piscitelli Mansur, A A [Department of Metallurgical and Materials Engineering, Biomaterials and Tissue Engineering Laboratory, Federal University of Minas Gerais, Belo Horizonte, MG (Brazil); Vasconcelos, W L [Department of Metallurgical and Materials Engineering, Biomaterials and Tissue Engineering Laboratory, Federal University of Minas Gerais, Belo Horizonte, MG (Brazil); Mansur, H S [Department of Metallurgical and Materials Engineering, Biomaterials and Tissue Engineering Laboratory, Federal University of Minas Gerais, Belo Horizonte, MG (Brazil)

2006-12-01

The purpose of this study was to develop novel hybrid organic-inorganic materials based on poly(vinyl alcohol) (PVA) polymer chemically crosslinked network to be tested as solid support on bovine herpesvirus immunoassay. Hybrids were synthesized by reacting PVA with three different alkoxysilanes modifying chemical groups: tetraethoxysilane (TEOS), 3-mercaptopropyltrimethoxysilane (MPTMS) and 3-glycidoxypropyltrimethoxysilane (GPTMS). PVA-derived hybrids were also modified by chemically crosslinking with glutaraldehyde (GA) during the synthesis reaction. In order to investigate the structure in the nanometer-scale, PVA-derived hybrids were characterized by using small-angle x-ray scattering synchrotron radiation (SAXS) and x-ray diffraction (XRD). PVA hybrids' chemical functionalities and their interaction with herpesviruses were also characterized by Fourier transform infrared spectroscopy (FTIR). The bioactivity assays were tested through enzyme linked immunosorbent assay (ELISA). SAXS results have indicated nano-ordered disperse domains for PVA hybrids with different x-ray scattering patterns for PVA polymer and PVA-derived hybrids. FTIR spectra have shown major vibration bands associated with organic-inorganic chemical groups present in the PVA, PVA-derived by silane modifier and PVA chemically crosslinked by GA. The immunoassay results have shown that PVA hybrids with chemically functionalized structures regulated to some extent the specific bioimmobilization of herpesvirus onto solid phase. We think that it is due to the overall balance of forces associated with van der Waals interaction, hydrophilic and hydrophobic forces and steric hindrance acting at the surface. PVA and PVA-derived hybrid materials were successfully produced with GA crosslinking in a nanometer-scale network. Also, such a PVA-based material could be advantageously used in immunoassays with enhanced specificity for diagnosis.

Development of a prototype lateral flow immunoassay (LFI for the rapid diagnosis of melioidosis.

Directory of Open Access Journals (Sweden)

Raymond L Houghton

2014-03-01

Full Text Available Burkholderia pseudomallei is a soil-dwelling bacterium and the causative agent of melioidosis. Isolation of B. pseudomallei from clinical samples is the "gold standard" for the diagnosis of melioidosis; results can take 3-7 days to produce. Alternatively, antibody-based tests have low specificity due to a high percentage of seropositive individuals in endemic areas. There is a clear need to develop a rapid point-of-care antigen detection assay for the diagnosis of melioidosis. Previously, we employed In vivo Microbial Antigen Discovery (InMAD to identify potential B. pseudomallei diagnostic biomarkers. The B. pseudomallei capsular polysaccharide (CPS and numerous protein antigens were identified as potential candidates. Here, we describe the development of a diagnostic immunoassay based on the detection of CPS. Following production of a CPS-specific monoclonal antibody (mAb, an antigen-capture immunoassay was developed to determine the concentration of CPS within a panel of melioidosis patient serum and urine samples. The same mAb was used to produce a prototype Active Melioidosis Detect Lateral Flow Immunoassay (AMD LFI; the limit of detection of the LFI for CPS is comparable to the antigen-capture immunoassay (∼0.2 ng/ml. The analytical reactivity (inclusivity of the AMD LFI was 98.7% (76/77 when tested against a large panel of B. pseudomallei isolates. Analytical specificity (cross-reactivity testing determined that 97.2% of B. pseudomallei near neighbor species (35/36 were not reactive. The non-reactive B. pseudomallei strain and the reactive near neighbor strain can be explained through genetic sequence analysis. Importantly, we show the AMD LFI is capable of detecting CPS in a variety of patient samples. The LFI is currently being evaluated in Thailand and Australia; the focus is to optimize and validate testing procedures on melioidosis patient samples prior to initiation of a large, multisite pre-clinical evaluation.

Influence of backscattering on the spatial resolution of semiconductor X-ray detectors

International Nuclear Information System (INIS)

Hoheisel, M.; Korn, A.; Giersch, J.

2005-01-01

Pixelated X-ray detectors using semiconductor layers or scintillators as absorbers are widely used in high-energy physics, medical diagnosis, or non-destructive testing. Their good spatial resolution performance makes them particularly suitable for applications where fine details have to be resolved. Intrinsic limitations of the spatial resolution have been studied in previous simulations. These simulations focused on interactions inside the conversion layer. Transmitted photons were treated as a loss. In this work, we also implemented the structure behind the conversion layer to investigate the impact of backscattering inside the detector setup. We performed Monte Carlo simulations with the program ROSI (Roentgen Simulation) which is based on the well-established EGS4 algorithm. Line-spread functions of different fully implemented detectors were simulated. In order to characterize the detectors' spatial resolution, the modulation transfer functions (MTF) were calculated. The additional broadening of the line-spread function by carrier transport has been ignored in this work. We investigated two different detector types: a directly absorbing pixel detector where a semiconductor slab is bump-bonded to a readout ASIC such as the Medipix-2 setup with Si or GaAs as an absorbing semiconductor layer, and flat-panel detectors with a Se or a CsI converter. We found a significant degradation of the MTF compared to the case without backscattering. At energies above the K-edge of the backscattering material the spatial resolution drops and can account for the observed low-frequency drop of the MTF. Ignoring this backscatter effect might lead to misinterpretations of the charge sharing effect in counting pixel detectors

Transport properties and superconductivity in presence of backscattering

International Nuclear Information System (INIS)

Chen, H.; Mattis, D.

1992-01-01

In this paper, the authors achieve an exact evaluation of the Kubo formula for electrical resistivity, with a model in which random impurity scattering is parametrized by random back-scattering matrix elements. If the alloy is a superconductor, our theory allows us to correlate T c with the normal-phase resistivity. The results are in nice agreement with experiment

A Switchable Linker-Based Immunoassay for Ultrasensitive Visible Detection of Salmonella in Tomatoes.

Science.gov (United States)

Hahn, Jungwoo; Kim, Eunghee; You, Young Sang; Gunasekaran, Sundaram; Lim, Seokwon; Choi, Young Jin

2017-10-01

On-site detection for sensitive identification of foodborne pathogens on fresh produce with minimal use of specialized instrumentation is crucial to the food industry. A switchable linker (SL)-based immunoassay was designed for ultrasensitive on-site detection of Salmonella in tomato samples. The assay is based on large-scale aggregation of gold nanoparticles (GNPs), induced by a quantitative relationship among the biotinylated Salmonella polyclonal antibody (b-Ab) used as the SL, the functionalized GNPs, and Salmonella. Important factors such as the concentration of SLs, time required for large-scale aggregation, and selectivity of b-Ab were optimized to minimize the detection time (within 45 min with gentle agitation) and achieve the lowest limit of detection (LOD; 10 CFU/g in tomato samples) possible. This SL-based immunoassay with its relatively low LOD and short detection time may meet the need for rapid, simple, on-site analysis of pathogens in fresh produce. The novel switchable linker-based immunoassay is a rapid, specific, and sensitive method that has potential applications for routine diagnostics of Salmonella in tomato products. These advantages make it a practical approach for general use in the processing industry to detect Salmonella rapidly and to implement appropriate regulatory procedures. Furthermore, it could be applied to other fresh products including cantaloupe, strawberry, and cucumbers. © 2017 Institute of Food Technologists®.

On-chip signal amplification of magnetic bead-based immunoassay by aviating magnetic bead chains.

Science.gov (United States)

Jalal, Uddin M; Jin, Gyeong Jun; Eom, Kyu Shik; Kim, Min Ho; Shim, Joon S

2017-11-06

In this work, a Lab-on-a-Chip (LOC) platform is used to electromagnetically actuate magnetic bead chains for an enhanced immunoassay. Custom-made electromagnets generate a magnetic field to form, rotate, lift and lower the magnetic bead chains (MBCs). The cost-effective, disposable LOC platform was made with a polymer substrate and an on-chip electrochemical sensor patterned via the screen-printing process. The movement of the MBCs is controlled to improve the electrochemical signal up to 230% when detecting beta-type human chorionic gonadotropin (β-hCG). Thus, the proposed on-chip MBC-based immunoassay is applicable for rapid, qualitative electrochemical point-of-care (POC) analysis. Copyright © 2017 Elsevier B.V. All rights reserved.

Statistical properties of intensity of partially polarised semiconductor laser light backscattered by a single-mode optical fibre

International Nuclear Information System (INIS)

Alekseev, A E; Potapov, V T; Gorshkov, B G

2015-01-01

We report the results of studying statistical properties of the intensity of partially polarised coherent light backscattered by a single mode optical fibre. An expression is derived for the deviation of the backscattered light intensity depending on the scattering region length, the degree of the light source coherence and the degree of scattered light polarisation. It is shown that the backscattered light in a fibre scattered-light interferometer is partially polarised with the polarisation degree P = 1/3 in the case of external perturbations of the interferometer fibre. (scattering of light)

Linkage of biomolecules to solid phases for immunoassay

International Nuclear Information System (INIS)

Chapman, R.S.

1998-01-01

Topics covered by this lecture include a brief review of the principal methods of linkage of biomolecules to solid phase matrices. Copies of the key self explanatory slides are presented as figures together with reprints of two publications by the author dealing with a preferred chemistry for the covalent linkage of antibodies to hydroxyl and amino functional groups and the effects of changes in solid phase matrix and antibody coupling chemistry on the performance of a typical excess reagent immunoassay for thyroid stimulating hormone

Sensitivity improvement of a laser interferometer limited by inelastic back-scattering, employing dual readout

International Nuclear Information System (INIS)

Meinders, Melanie; Schnabel, Roman

2015-01-01

Inelastic back-scattering of stray light is a long-standing and fundamental problem in high-sensitivity interferometric measurements and a potential limitation for advanced gravitational-wave (GW) detectors. The emerging parasitic interferences cannot be distinguished from a scientific signal via conventional single readout. In this work, we propose the subtraction of inelastic back-scatter signals by employing dual homodyne detection on the output light, and demonstrate it for a table-top Michelson interferometer. The additional readout contains solely parasitic signals and is used to model the scatter source. Subtraction of the scatter signal reduces the noise spectral density and thus improves the measurement sensitivity. Our scheme is qualitatively different from the previously demonstrated vetoing of scatter signals and opens a new path for improving the sensitivity of future GW detectors and other back-scatter limited devices. (paper)

Validation Test of Geant4 Simulation of Electron Backscattering

CERN Document Server

Kim, Sung Hun; Basaglia, Tullio; Han, Min Cheol; Hoff, Gabriela; Kim, Chan Hyeong; Saracco, Paolo

2015-01-01

Backscattering is a sensitive probe of the accuracy of electron scattering algorithms implemented in Monte Carlo codes. The capability of the Geant4 toolkit to describe realistically the fraction of electrons backscattered from a target volume is extensively and quantitatively evaluated in comparison with experimental data retrieved from the literature. The validation test covers the energy range between approximately 100 eV and 20 MeV, and concerns a wide set of target elements. Multiple and single electron scattering models implemented in Geant4, as well as preassembled selections of physics models distributed within Geant4, are analyzed with statistical methods. The evaluations concern Geant4 versions from 9.1 to 10.1. Significant evolutions are observed over the range of Geant4 versions, not always in the direction of better compatibility with experiment. Goodness-of-fit tests complemented by categorical analysis tests identify a configuration based on Geant4 Urban multiple scattering model in Geant4 vers...

Investigation of sheared liquids by neutron backscattering and reflectivity

CERN Document Server

Wolff, M; Hock, R; Frick, B; Zabel, H

2002-01-01

We have investigated by neutron scattering structural and dynamical properties of water solutions of the triblock copolymer P85 under shear. To this end a shear cell that suits the requirements for neutron backscattering and another for reflectivity experiments have been built. In reflectivity measurements we find the polymer concentration (nominal concentration of 33% by weight) to vary right at the surface between 12% and 52% for hydrophilic or hydrophobic coated silicon wavers, for temperatures between 18 C and 73 C and for shear rates up to 2500 s sup - sup 1. Additional structural changes deeper in the bulk are also observed. On the backscattering instrument (IN10 at ILL) we find that the liquid appears to stick to the plates of the shear cell, implying an unusual macroscopic velocity distribution that differs from that found earlier for lubrication oils. We report further on changes of the quasielastic line width in the direction of the shear gradient for different temperatures and shear rates. (orig.)

High-precision thickness measurements using beta backscatter

International Nuclear Information System (INIS)

Heckman, R.V.

1978-11-01

A two-axis, automated fixture for use with a high-intensity Pm-147 source and a photomultiplier-scintillation beta-backscatter probe for making thickness measurements has been designed and built. A custom interface was built to connect the system to a minicomputer, and software was written to position the tables, control the probe, and make the measurements. Measurements can be made in less time with much greater precision than by the method previously used

Analytical expressions for the electron backscattering coefficient

International Nuclear Information System (INIS)

August, H.J.; Wernisch, J.

1989-01-01

Several analytical expressions for the electron backscattering coefficient for massive homogeneous samples are compared with experimental data, directing special attention to the dependence of this quantity on the electron acceleration energy. It is shown that this dependence generally cannot be neglected. The expression proposed by Hunger and Kuechler turns out to be better than that of Love and Scott, although even the better formula can be slightly improved by a small modification. (author)

Variable solar control using thermotropic core/shell particles

Energy Technology Data Exchange (ETDEWEB)

Muehling, Olaf; Seeboth, Arno; Ruhmann, Ralf; Potechius, Elvira; Vetter, Renate [Fraunhofer Institute for Applied Polymer Research (IAP), Department of Chromogenic Polymers, Volmerstr. 7B, 12489 Berlin (Germany); Haeusler, Tobias [Brandenburg University of Technology (BTU Cottbus), Chair of Applied Physics/Thermophysics, Konrad-Zuse-Str. 1, 03046 Cottbus (Germany)

2009-09-15

Subject of our recent investigations is the utilization of a reversible thermotropic material for a self-regulating sun protection glazing that controls the solar energy input in order to avoid overheating. Based on the well-established UV curing technology for laminated glass a superior thermotropic material with tunable switching characteristics and of low material costs was developed. The polymer layer contains core/shell particles homogeneously dispersed in a UV-cured resin. The particle core in turn consists of an n-alkane mixture that is responsible for the temperature-induced clear/opaque switching. To obtain particles of well-defined size and with a narrow size distribution, the miniemulsion polymerization technique was used. The visible and solar optical properties (normal-normal, normal-hemispherical, and normal-diffuse transmittance) in the off (clear) and in the on state (opaque) were determined by UV/Vis/NIR spectroscopy. Samples containing particles of high median diameter (>800 nm) primarily scatter in the forward direction. However, with smaller particles (300-600 nm) a higher backscattering (reflection) efficiency was achieved. The largest difference in the normal-hemispherical transmittance could be found with a particle amount of 6% and a median scattering domain diameter of {proportional_to}380 nm. (author)

Diagnostic effectiveness of immunoassays systems for hepatitis C virus in samples from multi-transfusion patients

International Nuclear Information System (INIS)

Rivero Jimenez, Rene A; Merlin Linares, Julio C; Blanco de Armas, Madelin; Navea Leyva, Leonor M

2009-01-01

Hepatitis C virus (CHV) blood-transmission is a health problem in Cuba and in the world. Some types of diagnostic immunoassays have been developed for the blood certification and in general have a high diagnostic sensitivity and specificity in healthy donors. However, its behavior in samples from multi-transfusion patients could by less effective. To assess the diagnostic effectiveness of the UMELISA HCV third generation Cuban immunoassay (TecnoSUMA, S.A. La Habana), Cuba) in samples from multi-transfusion patients, in parallel, 335 sera from patients were processed by UBI HCV EIA 4.0 (United Biomedical, EE.UU) and UMELISA HCV third generation, and the samples with incongruous results were verified by PCR COBAS AmpliScreen HCV Test, v2 system (Roche, EE.UU.) Comparing the UMELISA HCV third generation system with the UBI HCV EIA 4.0 it was achieved a Sd of 95,8% CI(95%): 92,5-99,15 and a Ed of 100% CI (95%): 99,7-100, with IY: 0,96 (0,93-0,99) with k: 0,0582 ID (95%): 0,9276-0,9888, p = 0,000. Both immunoassay systems were satisfactory for immunodiagnosis of multi-transfusion patients

Evaluation of Four Supervised Learning Methods for Benthic Habitat Mapping Using Backscatter from Multi-Beam Sonar

Directory of Open Access Journals (Sweden)

Jacquomo Monk

2012-11-01

Full Text Available An understanding of the distribution and extent of marine habitats is essential for the implementation of ecosystem-based management strategies. Historically this had been difficult in marine environments until the advancement of acoustic sensors. This study demonstrates the applicability of supervised learning techniques for benthic habitat characterization using angular backscatter response data. With the advancement of multibeam echo-sounder (MBES technology, full coverage datasets of physical structure over vast regions of the seafloor are now achievable. Supervised learning methods typically applied to terrestrial remote sensing provide a cost-effective approach for habitat characterization in marine systems. However the comparison of the relative performance of different classifiers using acoustic data is limited. Characterization of acoustic backscatter data from MBES using four different supervised learning methods to generate benthic habitat maps is presented. Maximum Likelihood Classifier (MLC, Quick, Unbiased, Efficient Statistical Tree (QUEST, Random Forest (RF and Support Vector Machine (SVM were evaluated to classify angular backscatter response into habitat classes using training data acquired from underwater video observations. Results for biota classifications indicated that SVM and RF produced the highest accuracies, followed by QUEST and MLC, respectively. The most important backscatter data were from the moderate incidence angles between 30° and 50°. This study presents initial results for understanding how acoustic backscatter from MBES can be optimized for the characterization of marine benthic biological habitats.

A review of promising new immunoassay technology for monitoring forest herbicides

Science.gov (United States)

Charles K. McMahon

1993-01-01

Rising costs of classical instrumental methods of chemical analysis coupled with an increasing need for environmental monitoring has lead to the development of highly sensitive, low-cost immunochemical methods of analysis for the detection of environmental contaminants. These methods known simply as immunoassays are chemical assays which use antibodies as reagents. A...

Full aperture backscatter diagnostic for the NIF laser facility (abstract)

International Nuclear Information System (INIS)

Sewall, Noel; Lewis, Izzy; Kirkwood, Robert; Moody, John; Celeste, John

2001-01-01

The current schemes for achieving ignition on the National Ignition Facility require efficient coupling of energy from 192 laser beams to the deuterium--tritium fuel capsule. Each laser beam must propagate through a long scalelength plasma region before being converted to x rays (indirect drive) or being absorbed on the capsule (direct drive). Laser-plasma instabilities such as stimulated Brillouin and stimulated Raman scattering (SBS and SRS) will scatter a fraction of the incident laser energy out of the target leading to an overall reduction in the coupling efficiency. It is important to measure the character of this scattered light in order to understand it and to develop methods for reducing it to acceptable levels. We are designing a system called the full aperature backscatter diagnostic with the capability to measure the time-dependent amplitude and spectral content of the light which is backscattered through the incident beam focusing optic. The backscattered light will be collected over about 85% of the full beam aperture and separated into the SBS wavelength band (348--354 nm) and the SRS wavelength band (400--700 nm). Spectrometers coupled to streak cameras will provide time-resolved spectra for both scattered light components. The scattered light amplitude will be measured with fast and slow diodes. The entire system will be routinely calibrated. Analysis of the data will provide important information for reducing scattered power, achieving power balance, and finally achieving ignition

Trends in interfacial design for surface plasmon resonance based immunoassays

International Nuclear Information System (INIS)

Shankaran, Dhesingh Ravi; Miura, Norio

2007-01-01

Immunosensors based on surface plasmon resonance (SPR) have become a promising tool in sensor technology for biomedical, food, environmental, industrial and homeland security applications. SPR is a surface sensitive optical technique, suitable for real-time and label-free analysis of biorecognition events at functional transducer surfaces. Fabrication of highly active and robust sensing surfaces is an important part in immunoassays because the quality, quantity, chemistry and topography of the interfacial biomembranes play a major role in immunosensor performance. Eventually, a variety of immobilization methods such as physical adsorption, covalent coupling, Langmuir-Blodgett film, polymer thin film, self-assembly, sol-gel, etc, have been introduced over the years for the immobilization of biomolecules (antibody or antigen) on the transducer surfaces. The selection of an immobilization method for an immunoassay is governed by several factors such as nature and stability of the biomolecules, target analyte, application, detection principle, mode of signal transduction, matrix complexity, etc. This paper provides an overview of the various surface modification methods for SPR based immunosensor fabrication. The preparation, structure and application of different functional interfacial surfaces have been discussed along with a brief introduction to the SPR technology, biomolecules and detection principles. (review article)

Trends in interfacial design for surface plasmon resonance based immunoassays

Energy Technology Data Exchange (ETDEWEB)

Shankaran, Dhesingh Ravi [Art, Science and Technology Center for Cooperative Research, Kyushu University, Kasuga-shi, Fukuoka, 816-8580 (Japan); Miura, Norio [Art, Science and Technology Center for Cooperative Research, Kyushu University, Kasuga-shi, Fukuoka, 816-8580 (Japan)

2007-12-07

Immunosensors based on surface plasmon resonance (SPR) have become a promising tool in sensor technology for biomedical, food, environmental, industrial and homeland security applications. SPR is a surface sensitive optical technique, suitable for real-time and label-free analysis of biorecognition events at functional transducer surfaces. Fabrication of highly active and robust sensing surfaces is an important part in immunoassays because the quality, quantity, chemistry and topography of the interfacial biomembranes play a major role in immunosensor performance. Eventually, a variety of immobilization methods such as physical adsorption, covalent coupling, Langmuir-Blodgett film, polymer thin film, self-assembly, sol-gel, etc, have been introduced over the years for the immobilization of biomolecules (antibody or antigen) on the transducer surfaces. The selection of an immobilization method for an immunoassay is governed by several factors such as nature and stability of the biomolecules, target analyte, application, detection principle, mode of signal transduction, matrix complexity, etc. This paper provides an overview of the various surface modification methods for SPR based immunosensor fabrication. The preparation, structure and application of different functional interfacial surfaces have been discussed along with a brief introduction to the SPR technology, biomolecules and detection principles. (review article)

Universal quantum dot-based sandwich-like immunoassay strategy for rapid and ultrasensitive detection of small molecules using portable and reusable optofluidic nano-biosensing platform

International Nuclear Information System (INIS)

Zhou, Liping; Zhu, Anna; Lou, Xuening; Song, Dan; Yang, Rong; Shi, Hanchang; Long, Feng

2016-01-01

A universal sandwich-like immunoassay strategy based on quantum-dots immunoprobe (QD-labeled anti-mouse IgG antibody) was developed for rapid and ultrasensitive detection of small molecules. A portable and reusable optofluidic nano-biosensing platform was applied to investigate the sandwich-like immunoassay mechanism and format of small molecules, as well as the binding kinetics between QD immunoprobe and anti-small molecule antibody. A two-step immunoassay method that involves pre-incubation mixture of different concentration of small molecule and anti-small molecule antibody, and subsequent introduction of QD immunoprobe into the optofluidic cell was conducted for small molecule determination. Compared with the one-step immunoassay method, the two-step immunoassay method can obtain higher fluorescence signal and higher sensitivity index, thus improving the nano-biosensing performance. Based on the proposed strategy, two mode targets, namely, microcystin-LR (MC-LR) and Bisphenol A (BPA) were tested with high sensitivity, rapidity, and ease of use. A higher concentration of small molecules in the sample led to less anti-small molecule antibody bound with antigen-carrier protein conjugate immobilized onto the sensor surface, and less QD immunoprobes bound with anti-small molecule antibody. This phenomenon lowered the fluorescence signal detected by nano-biosensing platform. Under optimal operating conditions, MC-LR and BPA exhibited a limit of detection of 0.003 and 0.04 μg/L, respectively. The LODs were better than those of the indirect competitive immunoassay method for small molecules via Cy5.5-labeled anti-small molecule antibody. The proposed QD-based sandwich-like immunoassay strategy was evaluated in spiked water samples, and showed good recovery, precision and accuracy without complicated sample pretreatments. All these results demonstrate that the new detection strategy could be readily applied to the other trace small molecules in real water samples

Universal quantum dot-based sandwich-like immunoassay strategy for rapid and ultrasensitive detection of small molecules using portable and reusable optofluidic nano-biosensing platform

Energy Technology Data Exchange (ETDEWEB)

Zhou, Liping; Zhu, Anna; Lou, Xuening; Song, Dan; Yang, Rong [School of Environment and Natural Resources, Renmin University of China, Beijing (China); Shi, Hanchang [School of Environment, Tsinghua University, Beijing (China); Long, Feng, E-mail: longf04@ruc.edu.cn [School of Environment and Natural Resources, Renmin University of China, Beijing (China)

2016-01-28

A universal sandwich-like immunoassay strategy based on quantum-dots immunoprobe (QD-labeled anti-mouse IgG antibody) was developed for rapid and ultrasensitive detection of small molecules. A portable and reusable optofluidic nano-biosensing platform was applied to investigate the sandwich-like immunoassay mechanism and format of small molecules, as well as the binding kinetics between QD immunoprobe and anti-small molecule antibody. A two-step immunoassay method that involves pre-incubation mixture of different concentration of small molecule and anti-small molecule antibody, and subsequent introduction of QD immunoprobe into the optofluidic cell was conducted for small molecule determination. Compared with the one-step immunoassay method, the two-step immunoassay method can obtain higher fluorescence signal and higher sensitivity index, thus improving the nano-biosensing performance. Based on the proposed strategy, two mode targets, namely, microcystin-LR (MC-LR) and Bisphenol A (BPA) were tested with high sensitivity, rapidity, and ease of use. A higher concentration of small molecules in the sample led to less anti-small molecule antibody bound with antigen-carrier protein conjugate immobilized onto the sensor surface, and less QD immunoprobes bound with anti-small molecule antibody. This phenomenon lowered the fluorescence signal detected by nano-biosensing platform. Under optimal operating conditions, MC-LR and BPA exhibited a limit of detection of 0.003 and 0.04 μg/L, respectively. The LODs were better than those of the indirect competitive immunoassay method for small molecules via Cy5.5-labeled anti-small molecule antibody. The proposed QD-based sandwich-like immunoassay strategy was evaluated in spiked water samples, and showed good recovery, precision and accuracy without complicated sample pretreatments. All these results demonstrate that the new detection strategy could be readily applied to the other trace small molecules in real water samples

False-positive ethyl glucuronide immunoassay screening caused by a propyl alcohol-based hand sanitizer.

Science.gov (United States)

Arndt, Torsten; Grüner, Joachim; Schröfel, Stefanie; Stemmerich, Karsten

2012-11-30

Urine ethyl glucuronide (EtG) is considered as a specific marker of recent ethanol consumption. We describe false-positive DRI(®) EIA EtG enzyme immunoassay results caused by propyl glucuronides in urine after using a propanol-based hand sanitizer. EtG screening was done with the DRI(®) EIA EtG assay (Microgenics), using a cut-off of 0.5 mg/L as recommended by the manufacturer and of 0.1 mg/L as demanded by the German Regulations for Reissuing Drivers Licenses. Confirmatory EtG analysis was done with the ClinMass(®) EtG LC-MS/MS testkit (Recipe), extended by the mass transitions 235.1→75.1, 235.1→85.1, and 235.1→113.1 for the detection of the 1- and 2-propyl glucuronides. Self-experiments were done by staff members of our lab (n=7), using 3 mL Sterillium(®) Classic Pure (30 g/100 g 1-propanol and 45 g/100 g 2-propanol) for hand sanitation every quarter of an hour for 8 h according to DIN EN 1500:2011-05 with and without an exhauster and by passive inhalation of the sanitizer vapor. Spot urine samples were taken immediately before and up to 24 h after the first sanitizer use. False-positive immunoassay results of up to 4 mg/L or 2.3 mg/g creatinine were obtained after normal use of the sanitizer and also after passive inhalation of the sanitizer vapor (up to 0.89 mg/L or 0.61 mg/g). Immunoassay results were positive even after 4-fold use of the sanitizer (up to 0.14 mg/L or 0.38 mg/g) and up to 6 h after the last sanitizer contact (maximum 0.63 mg/L and 0.33 mg/g for sanitizer users and 0.25 mg/g after passive inhalation). Spiking of EtG-free urine with 1-propyl glucuronide (Athena Environmental Sciences) between 0.05 and 10 mg/L clearly demonstrated a cross reaction of the immunoassay of approx. 10% as compared to EtG. LC-MS/MS of urines with a positive immunoassay EtG result did not show EtG signals, but distinct signals of 1-propyl glucuronide (n-propyl glucuronide) and 2-propyl glucuronide (iso-propyl glucuronide). An exhauster effectively prevented

Simultaneous observations at different altitudes of ionospheric backscatter in the eastward electrojet

Directory of Open Access Journals (Sweden)

S. E. Milan

1998-01-01

Full Text Available A common feature of evening near-range ionospheric backscatter in the CUTLASS Iceland radar field of view is two parallel, approximately L-shell-aligned regions of westward flow which are attributed to irregularities in the auroral eastward electrojet region of the ionosphere. These backscatter channels are separated by approximately 100–200 km in range. The orientation of the CUTLASS Iceland radar beams and the zonally aligned nature of the flow allows an approximate determination of flow angle to be made without the necessity of bistatic measurements. The two flow channels have different azimuthal variations in flow velocity and spectral width. The nearer of the two regions has two distinct spectral signatures. The eastern beams detect spectra with velocities which saturate at or near the ion-acoustic speed, and have low spectral widths (less than 100 m s–1, while the western beams detect lower velocities and higher spectral widths (above 200 m s–1. The more distant of the two channels has only one spectral signature with velocities above the ion-acoustic speed and high spectral widths. The spectral characteristics of the backscatter are consistent with E-region scatter in the nearer channel and upper-E-region or F-region scatter in the further channel. Temporal variations in the characteristics of both channels support current theories of E-region turbulent heating and previous observations of velocity-dependent backscatter cross-section. In future, observations of this nature will provide a powerful tool for the investigation of simultaneous E- and F-region irregularity generation under similar (nearly co-located or magnetically conjugate electric field conditions.Key words. Auroral ionosphere · Ionospheric irregularities · Plasma convection

Gold nano-particles fixed on glass

International Nuclear Information System (INIS)

Worsch, Christian; Wisniewski, Wolfgang; Kracker, Michael; Rüssel, Christian

2012-01-01

Highlights: ► We produced wear resistant gold–ruby coatings on amorphous substrates. ► Thin sputtered gold layers were covered by or embedded in silica coatings. ► Annealing above T g of the substrate glass led to the formation of gold nano particles. ► A 1 1 1-texture of the gold particles is observed via XRD and EBSD. ► EBSD-patterns can be acquired from crystals covered by a thin layer of glass. - Abstract: A simple process for producing wear resistant gold nano-particle coatings on transparent substrates is proposed. Soda-lime-silica glasses were sputtered with gold and subsequently coated with SiO 2 using a combustion chemical vapor deposition technique. Some samples were first coated with silica, sputtered with gold and then coated with a second layer of silica. The samples were annealed for 20 min at either 550 or 600 °C. This resulted in the formation of round, well separated gold nano-particles with sizes from 15 to 200 nm. The color of the coated glass was equivalent to that of gold–ruby glasses. Silica/gold/silica coatings annealed at 600 °C for 20 min were strongly adherent and scratch resistant. X-ray diffraction and electron backscatter diffraction (EBSD) were used to describe the crystal orientations of the embedded particles. The gold particles are preferably oriented with their (1 1 1) planes perpendicular to the surface.

Magnetic bead and gold nanoparticle probes based immunoassay for β-casein detection in bovine milk samples.

Science.gov (United States)

Li, Y S; Meng, X Y; Zhou, Y; Zhang, Y Y; Meng, X M; Yang, L; Hu, P; Lu, S Y; Ren, H L; Liu, Z S; Wang, X R

2015-04-15

In this work, a double-probe based immunoassay was developed for rapid and sensitive determination of β-casein in bovine milk samples. In the method, magnetic beads (MBs), employed as supports for the immobilization of anti-β-casein polyclonal antibody (PAb), were used as the capture probe. Colloidal gold nanoparticles (AuNPs), employed as a bridge for loading anti-β-casein monoclonal antibody (McAb) and horseradish peroxidase (HRP), were used as the amplification probe. The presence of β-casein causes the sandwich structures of MBs-PAb-β-casein-McAb-AuNPs through the interaction between β-casein and the anti-β-casein antibodies. The HRP, used as an enzymatic-amplified tracer, can catalytically oxidize the substrate 3,3',5,5'-tetramethylbenzidine (TMB), generating optical signals that are proportional to the quantity of β-casein. The linear range of the immunoassay was from 6.5 to 1520ngmL(-1). The limit of detection (LOD) was 4.8ngmL(-1) which was 700 times lower than that of MBs-antibody-HRP based immunoassay and 6-7 times lower than that from the microplate-antibody-HRP based assay. The recoveries of β-casein from bovine milk samples were from 95.0% to 104.3% that had a good correlation coefficient (R(2)=0.9956) with those obtained by an official standard Kjeldahl method. For higher sensitivity, simple sample pretreatment and shorter time requirement of the antigen-antibody reaction, the developed immunoassay demonstrated the viability for detection of β-casein in bovine milk samples. Copyright © 2014. Published by Elsevier B.V.

The use of multibeam backscatter intensity data as a tool for mapping glacial deposits in the Central North Sea, UK

Science.gov (United States)

Stewart, Heather; Bradwell, Tom

2014-05-01

Multibeam backscatter intensity data acquired offshore eastern Scotland and north-eastern England have been used to map drumlin fields, large arcuate moraine ridges, smaller scale moraine ridges, and incised channels on the sea floor. The study area includes the catchments of the previously proposed, but only partly mapped, Strathmore, Forth-Tay, and Tweed palaeo-ice streams. The ice sheet glacial landsystem is extremely well preserved on the sea bed and comprehensive mapping of the seafloor geomorphology has been undertaken. The authors demonstrate the value in utilising not only digital terrain models (both NEXTMap and multibeam bathymetry derived) in undertaking geomorphological mapping, but also examining the backscatter intensity data that is often overlooked. Backscatter intensity maps were generated using FM Geocoder by the British Geological Survey. FM Geocoder corrects the backscatter intensities registered by the multibeam echosounder system, and then geometrically corrects and positions each acoustic sample in a backscatter mosaic. The backscatter intensity data were gridded at the best resolution per dataset (between 2 and 5 m). The strength of the backscattering is dependent upon sediment type, grain size, survey conditions, sea-bed roughness, compaction and slope. A combination of manual interpretation and semi-automated classification of the backscatter intensity data (a predictive method for mapping variations in surficial sea-bed sediments) has been undertaken in the study area. The combination of the two methodologies has produced a robust glacial geomorphological map for the study area. Four separate drumlin fields have been mapped in the study area indicative of fast-flowing and persistent ice-sheet flow configurations. A number of individual drumlins are also identified located outside the fields. The drumlins show as areas of high backscatter intensity compared to the surrounding sea bed, indicating the drumlins comprise mixed sediments of

Backscatter factor and absorption ratio of fibrous zirconia media in the visible

International Nuclear Information System (INIS)

Njomo, Donatien; Tagne, Herve Thierry Kamdem

2001-11-01

Fibrous thermal insulations are widely used to conserve energy in ambient to high temperature applications including buildings, solar collectors, heat exchangers, furnaces and thermal protection systems of reusable launch vehicles. It has long been recognised that zirconia has the lowest thermal conductivity of commercial refractories. The thermal conductivity of a zirconia fibrous medium is strongly dependent of its bulk density; high bulk densities of zirconia fibers provide the most effective insulation at high temperatures. Lee's theory for radiative transfer through fibrous media is used in this paper. The two-flux model is applied to determine the backward and forward parameters of a medium of zirconia fibers oriented in parallel planes. Theoretical calculations of the backscatter factor and absorption ratio of this medium are carried out in the visible spectrum for different size parameters of the fibers and for three different temperatures. Our results show that the backscatter factor of zirconia fibrous insulations is maximum, and therefore the heat transfer by the fibrous medium is the lowest, for a size parameter of 0.45 for all the temperatures studied. We also observed that the backscatter factor decreases with increasing temperature. (author)

Seabottom characterization using multibeam echosounder angular backscatter: An application of the composite roughness theory

Digital Repository Service at National Institute of Oceanography (India)

Chakraborty, B.; Schenke, H.W.; Kodagali, V.N.; Hagen, R.

multibeam echosounding systems reveal significant results related to seabottom geological processes ([3] and references therein). Jackson et al., [1] had proposed simultaneous application of the two backscatter theories related to the large and small-scale... to acquire multibeam deep ocean seabottom backscatter data of higher angular range (62 20 14 incidence angle). However, with the commercial availability of the multibeam-Hydrosweep system [4], which operates at a 45 14 half fan width, it has become possible...

Investigating the spectral characteristics of backscattering from heterogeneous spheroidal nuclei using broadband finite-difference time-domain simulations

Science.gov (United States)

Chao, Guo-Shan; Sung, Kung-Bin

2010-02-01

Backscattered light spectra have been used to extract size distribution of cell nuclei in epithelial tissues for noninvasive detection of precancerous lesions. In existing experimental studies, size estimation is achieved by assuming nuclei as homogeneous spheres or spheroids and fitting the measured data with models based on Mie theory. However, the validity of simplifying nuclei as homogeneous spheres has not been thoroughly examined. In this study, we investigate the spectral characteristics of backscattering from models of spheroidal nuclei under plane wave illumination using three-dimensional finite-difference time-domain (FDTD) simulation. A modulated Gaussian pulse is used to obtain wavelength dependent scattering intensity with a single FDTD run. The simulated model of nuclei consists of a nucleolus and randomly distributed chromatin condensation in homogeneous cytoplasm and nucleoplasm. The results show that backscattering spectra from spheroidal nuclei have similar oscillating patterns to those from homogeneous spheres with the diameter equal to the projective length of the spheroidal nucleus along the propagation direction. The strength of backscattering is enhanced in heterogeneous spheroids as compared to homogeneous spheroids. The degree of which backscattering spectra of heterogeneous nuclei deviate from Mie theory is highly dependent on the distribution of chromatin/nucleolus but not sensitive to nucleolar size, refractive index fluctuation or chromatin density.

Constraining the physical properties of Titan's empty lake basins using nadir and off-nadir Cassini RADAR backscatter

Science.gov (United States)

Michaelides, R. J.; Hayes, A. G.; Mastrogiuseppe, M.; Zebker, H. A.; Farr, T. G.; Malaska, M. J.; Poggiali, V.; Mullen, J. P.

2016-05-01

We use repeat synthetic aperture radar (SAR) observations and complementary altimetry passes acquired by the Cassini spacecraft to study the scattering properties of Titan's empty lake basins. The best-fit coefficients from fitting SAR data to a quasi-specular plus diffuse backscatter model suggest that the bright basin floors have a higher dielectric constant, but similar facet-scale rms surface facet slopes, to surrounding terrain. Waveform analysis of altimetry returns reveals that nadir backscatter returns from basin floors are greater than nadir backscatter returns from basin surroundings and have narrower pulse widths. This suggests that floor deposits are structurally distinct from their surroundings, consistent with the interpretation that some of these basins may be filled with evaporitic and/or sedimentary deposits. Basin floor deposits also express a larger diffuse component to their backscatter, which is likely due to variations in subsurface structure or an increase in roughness at the wavelength scale (Hayes, A.G. et al. [2008]. Geophys. Res. Lett. 35, 9). We generate a high-resolution altimetry radargram of the T30 altimetry pass over an empty lake basin, with which we place geometric constraints on the basin's slopes, rim heights, and depth. Finally, the importance of these backscatter observations and geometric measurements for basin formation mechanisms is briefly discussed.

Comparison of Immunoassay methods for T3, T4 and TSH

International Nuclear Information System (INIS)

Alonso Rodríguez, Celia A.

2016-01-01

Measurements of T3, T4 and TSH have been considered very important in the diagnosis and monitoring of thyroid diseases both overt and subclinical. These subclinical diseases are actively seeking for years, both in healthy patients and hospitalized for other illnesses; and in the population over 35 years, especially women, in health checkups. The active search for these diseases requires the use of rapid and reliable techniques; that can be developed massively, with good level of detectability and comparable. The overall objective is to present the evaluation of different immunoassay techniques with respect to the RIA and IRMA: ELISA, chemiluminescence, Amplified Chemiluminescence, electrochemiluminescence Immunofluorescence. Compare including automatic methods and analyze the cost and feasibility of them for laboratory immunoassay. ELISA colorimetric technique for dosing was comparable to RIA T4, not for T3. Chemiluminescence (AMERLITE) compared to dosing RIA and IRMA T4 to TSH proved to be valid for both. Amplified Chemiluminescence (Immulite) compared to IRMA for TSH was no significant difference. Electrochemiluminescence (Elecsys 2010) compared to T3 and T4 RIA and IRMA for TSH, no significant differences for T4 and TSH; but no variation to T3. Immunofluorescence (AIA-600) used to compare with RIA for T3 and T4, and TSH IRMA, no significant differences for the measured analytes. Benchmarking of automatic methods suggests that the most thrifty of trials is Immunofluorescence the AIA-600, regarding calibration and control, programming time, randomization and the ability to save the value of the fluorescence deferred calculations for tests without valid at the time of realizing calibration. Analyzing the cost and feasibility of these methods for laboratory immunoassay, we must consider that their characteristics electrochemiluminescence is the fastest, but its price is prohibitive for our health systems. The AIA-600 appears to be the method of choice for its

Variations in daily quality assurance dosimetry from device levelling, feet position and backscatter material

International Nuclear Information System (INIS)

Ceylan, Abdurrahman; Cullen, Ashley; Butson, Martin; Yu, Peter K.N.; Alnawaf, Hani

2012-01-01

Daily quality assurance procedures are an essential part of radiotherapy medical physics. Devices such as the Sun Nuclear, DQA3 are effective tools for analysis of daily dosimetry including flatness, symmetry, energy, field size and central axis radiation dose measurement. The DQA3 can be used on the treatment couch of the linear accelerator or on a dedicated table/bed for superficial and orthovoltage x-ray machines. This device is levelled using its dedicated feet. This work has shown that depending on the quantity of backscatter material behind the DQA3 device, the position of the levelling feet can affect the measured central axis dose by up to 1.8 % (250 kVp and 6 MV) and that the introduction of more backscatter material behind the DQA3 can lead to up to 7.2 % (6 MV) variations in measured central axis dose. In conditions where no backscatter material is present, dose measurements can vary up to 1 %. As such this work has highlighted the need to keep the material behind the DQA3 device constant as well as maintaining the accuracy of the feet position on the device to effectively measure the most accurate daily constancy achievable. Results have also shown that variations in symmetry and energy calculations of up to 1 % can occur if the device is not levelled appropriately. As such, we recommend the position of the levelling feet on the device be as close as possible to the device so that a constant distance is kept between the DQA3 and the treatment couch and thus minimal levelling variations also occur. We would also recommend having no extra backscattering material behind the DQA3 device during use to minimise any variations which might occur from these backscattering effects.

Biotin IgM Antibodies in Human Blood: A Previously Unknown Factor Eliciting False Results in Biotinylation-Based Immunoassays

Science.gov (United States)

Chen, Tingting; Hedman, Lea; Mattila, Petri S.; Jartti, Laura; Jartti, Tuomas; Ruuskanen, Olli; Söderlund-Venermo, Maria; Hedman, Klaus

2012-01-01

Biotin is an essential vitamin that binds streptavidin or avidin with high affinity and specificity. As biotin is a small molecule that can be linked to proteins without affecting their biological activity, biotinylation is applied widely in biochemical assays. In our laboratory, IgM enzyme immuno assays (EIAs) of µ-capture format have been set up against many viruses, using as antigen biotinylated virus like particles (VLPs) detected by horseradish peroxidase-conjugated streptavidin. We recently encountered one serum sample reacting with the biotinylated VLP but not with the unbiotinylated one, suggesting in human sera the occurrence of biotin-reactive antibodies. In the present study, we search the general population (612 serum samples from adults and 678 from children) for IgM antibodies reactive with biotin and develop an indirect EIA for quantification of their levels and assessment of their seroprevalence. These IgM antibodies were present in 3% adults regardless of age, but were rarely found in children. The adverse effects of the biotin IgM on biotinylation-based immunoassays were assessed, including four inhouse and one commercial virus IgM EIAs, showing that biotin IgM do cause false positivities. The biotin can not bind IgM and streptavidin or avidin simultaneously, suggesting that these biotin-interactive compounds compete for the common binding site. In competitive inhibition assays, the affinities of biotin IgM antibodies ranged from 2.1×10−3 to 1.7×10−4 mol/L. This is the first report on biotin antibodies found in humans, providing new information on biotinylation-based immunoassays as well as new insights into the biomedical effects of vitamins. PMID:22879954

A proportional counter for efficient backscatter Moessbauer effect spectroscopy

International Nuclear Information System (INIS)

Pawlowski, Z.; Marzec, J.; Cudny, W.; Holnicka, J.; Walentek, J.

1979-01-01

The authors present a novel gas-tight proportional counter with flat beryllium windows for backscatter Moessbauer spectroscopy. The krypton-filled counter has a geometry that approaches 2π and a resolution of 12% fwhm for the 14.4 keV line of 57 Fe, and is easy to manufacture. (Auth.)

Raman backscattering of circularly polarized electromagnetic waves propagating along a magnetic field

International Nuclear Information System (INIS)

Maraghechi, B.; Willett, J.e.

1979-01-01

The stimulated Raman backscattering of an intense electromagnetic wave propagating in the extraordinary mode along a uniform, static magnetic field is considered. The dispersion relation for a homogeneous magnetized plasma in the presence of the circularly polarized pump waves is developed in the cold-plasma approximation with the pump frequency above the plasma frequency. Formulas are derived for the threshold νsub(OT) of the parametric instability and for the growth rate γ of the backscattered extraordinary wave and Langmuir wave. The effects of the magnetic field parallel to the direction of propagation on νsub(0T) and γ are studied numerically. (author)

Development and analytical performance of a new ARCHITECT automated dipeptidyl peptidase-4 immunoassay

Directory of Open Access Journals (Sweden)

Philip M. Hemken

2017-12-01

Full Text Available Background: Dipeptidyl peptidase-4 (DPP-4 may be a suitable biomarker to identify people with severe asthma who have greater activation of the interleukin-13 (IL-13 pathway and may therefore benefit from IL-13-targeted treatments. We report the analytical performance of an Investigational Use Only immunoassay and provide data on the biological range of DPP-4 concentrations. Methods: We assessed assay performance, utilising analyses of precision, linearity and sensitivity; interference from common endogenous assay interferents, and from asthma and anti-diabetic medications, were also assessed. The assay was used to measure the range of serum DPP-4 concentrations in healthy volunteers and subjects with diabetes and severe, uncontrolled asthma. Results: The total precision of DPP-4 concentration measurement (determined using percentage coefficient of variation was ≤5% over 20 days. Dilution analysis yielded linear results from 30 to 1305 ng/mL; the limit of quantitation was 19.2 ng/mL. No notable endogenous or drug interferences were observed at the expected therapeutic concentration. Median DPP-4 concentrations in healthy volunteers and subjects with asthma or Type 1 diabetes were assessed, with concentrations remaining similar in subjects with diabetes and asthma across different demographics. Conclusion: These analyses indicate that the ARCHITECT DPP-4 Immunoassay is a reliable and robust method for measuring serum DPP-4 concentration. Keywords: Asthma, Automated immunoassay, Biomarker, Dipeptidyl peptidase-4, IL-13

Acoustic backscattering and radiation force on a rigid elliptical cylinder in plane progressive waves.

Science.gov (United States)

Mitri, F G

2016-03-01

This work proposes a formal analytical theory using the partial-wave series expansion (PWSE) method in cylindrical coordinates, to calculate the acoustic backscattering form function as well as the radiation force-per-length on an infinitely long elliptical (non-circular) cylinder in plane progressive waves. The major (or minor) semi-axis of the ellipse coincides with the direction of the incident waves. The scattering coefficients for the rigid elliptical cylinder are determined by imposing the Neumann boundary condition for an immovable surface and solving a resulting system of linear equations by matrix inversion. The present method, which utilizes standard cylindrical (Bessel and Hankel) wave functions, presents an advantage over the solution for the scattering that is ordinarily expressed in a basis of elliptical Mathieu functions (which are generally non-orthogonal). Furthermore, an integral equation showing the direct connection of the radiation force function with the square of the scattering form function in the far-field from the scatterer (applicable for plane waves only), is noted and discussed. An important application of this integral equation is the adequate evaluation of the radiation force function from a bistatic measurement (i.e., in the polar plane) of the far-field scattering from any 2D object of arbitrary shape. Numerical predictions are evaluated for the acoustic backscattering form function and the radiation force function, which is the radiation force per unit length, per characteristic energy density, and per unit cross-sectional surface of the ellipse, with particular emphasis on the aspect ratio a/b, where a and b are the semi-axes, as well as the dimensionless size parameter kb, without the restriction to a particular range of frequencies. The results are particularly relevant in acoustic levitation, acousto-fluidics and particle dynamics applications. Copyright © 2015 Elsevier B.V. All rights reserved.

Remote sensing of PM2.5 during cloudy and nighttime periods using ceilometer backscatter

Science.gov (United States)

Li, Siwei; Joseph, Everette; Min, Qilong; Yin, Bangsheng; Sakai, Ricardo; Payne, Megan K.

2017-06-01

Monitoring PM2.5 (particulate matter with aerodynamic diameter d ≤ 2.5 µm) mass concentration has become of more importance recently because of the negative impacts of fine particles on human health. However, monitoring PM2.5 during cloudy and nighttime periods is difficult since nearly all the passive instruments used for aerosol remote sensing are not able to measure aerosol optical depth (AOD) under either cloudy or nighttime conditions. In this study, an empirical model based on the regression between PM2.5 and the near-surface backscatter measured by ceilometers was developed and tested using 6 years of data (2006 to 2011) from the Howard University Beltsville Campus (HUBC) site. The empirical model can explain ˜ 56, ˜ 34 and ˜ 42 % of the variability in the hourly average PM2.5 during daytime clear, daytime cloudy and nighttime periods, respectively. Meteorological conditions and seasons were found to influence the relationship between PM2.5 mass concentration and the surface backscatter. Overall the model can explain ˜ 48 % of the variability in the hourly average PM2.5 at the HUBC site when considering the seasonal variation. The model also was tested using 4 years of data (2012 to 2015) from the Atmospheric Radiation Measurement (ARM) Southern Great Plains (SGP) site, which was geographically and climatologically different from the HUBC site. The results show that the empirical model can explain ˜ 66 and ˜ 82 % of the variability in the daily average PM2.5 at the ARM SGP site and HUBC site, respectively. The findings of this study illustrate the strong need for ceilometer data in air quality monitoring under cloudy and nighttime conditions. Since ceilometers are used broadly over the world, they may provide an important supplemental source of information of aerosols to determine surface PM2.5 concentrations.

Direct biosensor immunoassays for the detection of nonmilk proteins in milk powder

NARCIS (Netherlands)

Haasnoot, W.; Olieman, K.; Cazemier, G.; Verheijen, R.

2001-01-01

The low prices of some nonmilk proteins make them attractive as potential adulterants in dairy products. An optical biosensor (BIACORE 3000) was used to develop a direct and combined biosensor immunoassay (BIA) for the simultaneous detection of soy, pea, and soluble wheat proteins in milk powders.

Klein tunneling in carbon nanostructures: A free-particle dynamics in disguise

International Nuclear Information System (INIS)

Jakubsky, Vit; Nieto, Luis-Miguel; Plyushchay, Mikhail S.

2011-01-01

The absence of backscattering in metallic nanotubes as well as perfect Klein tunneling in potential barriers in graphene are the prominent electronic characteristics of carbon nanostructures. We show that the phenomena can be explained by a peculiar supersymmetry generated by a first order Hamiltonian and zero-order supercharge operators. Like the supersymmetry associated with second order reflectionless finite-gap systems, it relates here the low-energy behavior of the charge carriers with the free-particle dynamics.

A competitive chemiluminescence enzyme immunoassay for rapid and sensitive determination of enrofloxacin

Science.gov (United States)

Yu, Fei; Wu, Yongjun; Yu, Songcheng; Zhang, Huili; Zhang, Hongquan; Qu, Lingbo; Harrington, Peter de B.

With alkaline phosphatase (ALP)-adamantane (AMPPD) system as the chemiluminescence (CL) detection system, a highly sensitive, specific and simple competitive chemiluminescence enzyme immunoassay (CLEIA) was developed for the measurement of enrofloxacin (ENR). The physicochemical parameters, such as the chemiluminescent assay mediums, the dilution buffer of ENR-McAb, the volume of dilution buffer, the monoclonal antibody concentration, the incubation time, and other relevant variables of the immunoassay have been optimized. Under the optimal conditions, the detection linear range of 350-1000 pg/mL and the detection limit of 0.24 ng/mL were provided by the proposed method. The relative standard deviations were less than 15% for both intra and inter-assay precision. This method has been successfully applied to determine ENR in spiked samples with the recovery of 103%-96%. It showed that CLEIA was a good potential method in the analysis of residues of veterinary drugs after treatment of related diseases.

Screening for cocaine on Euro banknotes by a highly sensitive enzyme immunoassay.

Science.gov (United States)

Abdelshafi, Nahla A; Panne, Ulrich; Schneider, Rudolf J

2017-04-01

This study focused on quantitative detection of cocaine on Euro banknotes in Germany. A sensitive direct competitive immunoassay was developed and optimized with a limit of detection (LOD) of 5.6ng/L. Exhaustive cocaine extraction by solvent was tested using different methanol concentrations and buffered solutions. Cross-reactivity studies were performed to determine the degree of interference of cocaine metabolites with the immunoassay. Sixty-five Euro banknotes obtained from different districts in Berlin were evaluated. A 100% contamination frequency with cocaine was detected. A comparison between the amount of cocaine extracted by cotton swabbing of one square centimeter of the banknote showed a good correlation for lower contamination levels. This assay showed high sensitivity of detecting pg of cocaine per 1cm 2 of one banknote by swabbing 1cm 2 : 0, 14, and 21pg/cm 2 . Moreover, three notes of different denominations revealed high cocaine concentration; 1.1mg/note, and twice 55µg/note. Copyright © 2017 Elsevier B.V. All rights reserved.

Users guide to the HELIOS backscattering spectrometer (BSS)

International Nuclear Information System (INIS)

Bunce, L.J.

1986-10-01

The BSS is a backscattering spectrometer installed on the Harwell 136 Mev electron linear accelerator, HELIOS. A general description of the instrument is given, along with the time of flight scales, and the run and sample changer control units. The sample environment, vacuum system and detectors of the BSS are described, as well as the preparation, starting and running of an experiment using the BSS. (UK)

Simulation of Neutron Backscattering applied to organic material detection

International Nuclear Information System (INIS)

Forero, N. C.; Cruz, A. H.; Cristancho, F.

2007-01-01

The Neutron Backscattering technique is tested when performing the task of localizing hydrogenated explosives hidden in soil. Detector system, landmine, soil and neutron source are simulated with Geant4 in order to obtain the number of neutrons detected when several parameters like mine composition, relative position mine-source and soil moisture are varied

Tuberculin purified protein derivative (PPD) immunoassay as an in vitro alternative assay for identity and confirmation of potency.

Science.gov (United States)

Ho, Mei M; Kairo, Satnam K; Corbel, Michael J

2006-01-01

Tuberculin purified protein derivative (PPD) currently can only be standardised by delayed hypersensitivity skin reactions in sensitised guinea pigs. An in vitro dot blot immunoassay was developed for both identity and confirmation of potency estimation of PPD. Polyclonal antibodies (mainly IgG) were generated and immunoreacted with human, bovine and, to lesser extent, avian PPD preparations. Combining size exclusion chromatography (FPLC-SEC) and dot blot immunoassay, the results showed that PPD preparations were mixtures of very heterogeneous tuberculoproteins ranging in size from very large aggregates to very small degraded molecules. All individual fractions of PPD separated by size were immunoreactive, although those of the largest molecular sizes appeared the most immunoreactive in this in vitro dot blot immunoassay. This method is very sensitive and specific to tuberculoproteins and can be an in vitro alternative for the in vivo intradermal skin assay which uses guinea pigs for identity of PPD preparations. Although the capacity of PPD to elicit cell-mediated immune responses on intradermal testing has to be confirmed by in vivo assay, the dot blot immunoassay offers a rapid, sensitive and animal-free alternative to in vivo testing for confirming the identity of PPD preparations with appropriate potencies. This alternative assay would be particularly useful for national regulatory laboratories for confirming the data of manufacturers and thus reducing the use of animals.

Analysis of a nuclear backscattering and reaction data by the method of convolution integrals

International Nuclear Information System (INIS)

Lewis, M.B.

1979-02-01

A quantitative description of nuclear backscattering and reaction processes is made. Various formulas pertinent to nuclear microanalysis are assembled in a manner useful for experimental application. Convolution integrals relating profiles of atoms in a metal substrate to the nuclear reaction spectra obtained in the laboratory are described and computed. Energy straggling and multiple scattering are explicitly included and shown to be important. Examples of the application of the method to simple backscattering, oxide films, and implanted gas are discussed. 7 figures, 1 table

Development of Indirect Competitive Immuno-Assay Method Using SPR Detection for Rapid and Highly Sensitive Measurement of Salivary Cortisol Levels

International Nuclear Information System (INIS)

Tahara, Yusuke; Huang, Zhe; Kiritoshi, Tetsuro; Onodera, Takeshi; Toko, Kiyoshi

2014-01-01

The monitoring of salivary cortisol as a key biomarker of an individual’s stress response has been increasingly focused on. This paper describes the development of a novel cortisol immuno-assay method based on an indirect competitive method using a commercially available surface plasmon resonance instrument. The surface of an Au chip was modified with PEG6-COOH aromatic dialkanethiol self-assembled monolayers and hydrocortisone 3-(O-carboxymethyl) oxime (hydrocortisone 3-CMO) as a cortisol analog. A detection limit of 38 ppt range with a measurement range of 10 ppt–100 ppb was accomplished without the incubation of a mixing solution consisting of standard cortisol and an anti-cortisol antibody, and the time for quantification of cortisol concentration was 8 min from the sample injection. We experimentally compared our immuno-assay with a commercialized salivary cortisol enzyme-linked immunosorbent assay (ELISA) kit using human saliva samples. It was found that the results obtained by the cortisol immuno-assay had a good correlation with those obtained by ELISA assay (R = 0.96). Our findings indicate the potential utility of the cortisol immuno-assay for measurements of human salivary cortisol levels.

Development of Indirect Competitive Immuno-Assay Method Using SPR Detection for Rapid and Highly Sensitive Measurement of Salivary Cortisol Levels

Energy Technology Data Exchange (ETDEWEB)

Tahara, Yusuke; Huang, Zhe; Kiritoshi, Tetsuro [Graduate School of Information Science and Electrical Engineering, Kyushu University, Fukuoka (Japan); Onodera, Takeshi [Research and Development Center for Taste and Odor Sensing, Kyushu University, Fukuoka (Japan); Toko, Kiyoshi, E-mail: toko@ed.kyushu-u.ac.jp [Graduate School of Information Science and Electrical Engineering, Kyushu University, Fukuoka (Japan); Research and Development Center for Taste and Odor Sensing, Kyushu University, Fukuoka (Japan)

2014-05-30

The monitoring of salivary cortisol as a key biomarker of an individual’s stress response has been increasingly focused on. This paper describes the development of a novel cortisol immuno-assay method based on an indirect competitive method using a commercially available surface plasmon resonance instrument. The surface of an Au chip was modified with PEG6-COOH aromatic dialkanethiol self-assembled monolayers and hydrocortisone 3-(O-carboxymethyl) oxime (hydrocortisone 3-CMO) as a cortisol analog. A detection limit of 38 ppt range with a measurement range of 10 ppt–100 ppb was accomplished without the incubation of a mixing solution consisting of standard cortisol and an anti-cortisol antibody, and the time for quantification of cortisol concentration was 8 min from the sample injection. We experimentally compared our immuno-assay with a commercialized salivary cortisol enzyme-linked immunosorbent assay (ELISA) kit using human saliva samples. It was found that the results obtained by the cortisol immuno-assay had a good correlation with those obtained by ELISA assay (R = 0.96). Our findings indicate the potential utility of the cortisol immuno-assay for measurements of human salivary cortisol levels.

Transition to turbulence via spatiotemporal intermittency in stimulated Raman backscattering

International Nuclear Information System (INIS)

Skoric, M.M.; Jovanovic, M.S.; Rajkovic, M.R.

1996-01-01

The spatiotemporal evolution of stimulated Raman backscattering in a bounded, uniform, weakly dissipative plasma is studied. The nonlinear model of a three-wave interaction involves a quadratic coupling of slowly varying complex amplitudes of the laser pump, the backscattered and the electron plasma wave. The corresponding set of coupled partial differential equations with nonlinear phase detuning that is taken into account is solved numerically in space time with fixed nonzero source boundary conditions. The study of the above open, convective, weakly confined system reveals a quasiperiodic transition to spatiotemporal chaos via spatiotemporal intermittency. In the analysis of transitions a dual scheme borrowed from fields of nonlinear dynamics and statistical physics is applied. An introduction of a nonlinear three-wave interaction to a growing family of paradigmatic equations which exhibit a route to turbulence via spatiotemporal intermittency is outlined in this work. copyright 1996 The American Physical Society

Single biosensor immunoassay for the detection of five aminoglycosides in reconstituted skimmed milk

NARCIS (Netherlands)

Haasnoot, W.; Cazemier, G.; Koets, M.; Amerongen, van A.

2003-01-01

The application of an optical biosensor (Biacore 3000), with four flow channels (Fcs), in combination with a mixture of four specific antibodies resulted in a competitive inhibition biosensor immunoassay (BIA) for the simultaneous detection of the five relevant aminoglycosides in reconstituted

Estimation of mineral concentration in bone samples by backscattering of beta-rays

International Nuclear Information System (INIS)

Meissner, J.; Marten, R.F.

1978-01-01

The method of using backscattered β-rays for the determination of density is used for the first time to measure the mineral concentration in specimens of bone and its usefulness as an aid in the diagnosis of osteopathies is investigated. The reliability of the technique is studied on the basis of methodical examinations employing model substances that are similar in composition to bone. For the geometry chosen for the measureing set-up, a calibration is carried out both for the determination of the effective atomic number and for the mineral concentration of measuring samples. The axial-symmetrical arrangement chose, permits the determination of the concentration to an accuracy of max. +-1% standard deviation. In agreement with computed deviations experiments confirm that with this set-up there is no dependence of the backscatter on sample density. After appropriate calibration, both mineral concentration and density can be measured in two suitable fixed positions of the beta source, sample and detector. The figure for mineral concentration calculated from the backscattering rates are compared with corresponding figure obtained with analytical chemical determinations. It can be seen that two methods have a high degree of agreement. The backscatter method seems to have the advantage of simplicity of the set-up that provides accurate results in thin specimens having a diameter of only 10 mm. With repect to measurements in bone, however, the method is limited to biopsy specimens obtained from compact bone. In such samples, however, both the absolute figure for mineral concentration can be determined and also its local variation in large parts of the skeleton examined. From the mineral concentration and density, the mineral content of the bone substance is computed; this provides differential diagnostic information on osteopathic conditions. (orig.) [de

Estimation of organ and effective dose due to Compton backscatter security scans

International Nuclear Information System (INIS)

Hoppe, Michael E.; Schmidt, Taly Gilat

2012-01-01

Purpose: To estimate organ and effective radiation doses due to backscatter security scanners using Monte Carlo simulations and a voxelized phantom set. Methods: Voxelized phantoms of male and female adults and children were used with the GEANT4 toolkit to simulate a backscatter security scan. The backscatter system was modeled based on specifications available in the literature. The simulations modeled a 50 kVp spectrum with 1.0 mm-aluminum-equivalent filtration and a previously measured exposure of approximately 4.6 μR at 30 cm from the source. Photons and secondary interactions were tracked from the source until they reached zero kinetic energy or exited from the simulation’s boundaries. The energy deposited in the phantoms’ respective organs was tallied and used to calculate total organ dose and total effective dose for frontal, rear, and full scans with subjects located 30 and 75 cm from the source. Results: For a full screen, all phantoms’ total effective doses were below the established 0.25 μSv standard, with an estimated maximum total effective dose of 0.07 μSv for full screen of a male child. The estimated maximum organ dose due to a full screen was 1.03 μGy, deposited in the adipose tissue of the male child phantom when located 30 cm from the source. All organ dose estimates had a coefficient of variation of less than 3% for a frontal scan and less than 11% for a rear scan. Conclusions: Backscatter security scanners deposit dose in organs beyond the skin. The effective dose is below recommended standards set by the Health Physics Society (HPS) and the American National Standards Institute (ANSI) assuming the system provides a maximum exposure of approximately 4.6 μR at 30 cm.

Simultaneous observations at different altitudes of ionospheric backscatter in the eastward electrojet

Directory of Open Access Journals (Sweden)

S. E. Milan

Full Text Available A common feature of evening near-range ionospheric backscatter in the CUTLASS Iceland radar field of view is two parallel, approximately L-shell-aligned regions of westward flow which are attributed to irregularities in the auroral eastward electrojet region of the ionosphere. These backscatter channels are separated by approximately 100–200 km in range. The orientation of the CUTLASS Iceland radar beams and the zonally aligned nature of the flow allows an approximate determination of flow angle to be made without the necessity of bistatic measurements. The two flow channels have different azimuthal variations in flow velocity and spectral width. The nearer of the two regions has two distinct spectral signatures. The eastern beams detect spectra with velocities which saturate at or near the ion-acoustic speed, and have low spectral widths (less than 100 m s^–1, while the western beams detect lower velocities and higher spectral widths (above 200 m s^–1. The more distant of the two channels has only one spectral signature with velocities above the ion-acoustic speed and high spectral widths. The spectral characteristics of the backscatter are consistent with E-region scatter in the nearer channel and upper-E-region or F-region scatter in the further channel. Temporal variations in the characteristics of both channels support current theories of E-region turbulent heating and previous observations of velocity-dependent backscatter cross-section. In future, observations of this nature will provide a powerful tool for the investigation of simultaneous E- and F-region irregularity generation under similar (nearly co-located or magnetically conjugate electric field conditions.

Key words. Auroral ionosphere · Ionospheric irregularities · Plasma convection

Chemiluminescence immunoassay for prostate-specific antigen

International Nuclear Information System (INIS)

Zhang Xuefeng; Liu Yibing; Jia Juanjuan; Xu Wenge; Li Ziying; Chen Yongli; Han Shiquan

2008-01-01

The chemiluminescence immunoassay (CLIA) for serum total prostate-specific antigen (T-PSA) was developed. The reaction of luminol with hydrogen peroxide was introduced into this chemiluminescence system. The detection limit is established as 0.12 μg/L (n=10, mean of zero standard + 2SD) and the analytical recovery of PSA is 83.8%-118.7%. The intra-assay and inter-assay CVs vary from 4.4%-5.0% and 6.2%-11.7%, respectively. The experimental correlation coefficient of dilution is found to be 0.999. Compared with immunoradiometric assay (IRMA) kits, the correlative equation is y=1.07x+0.68, and correlation coefficient r=0.97. The standard range for the method is 1.5-80 μg/L, and it presents good linearity. (authors)

Inkjet-printed point-of-care immunoassay on a nanoscale polymer brush enables subpicomolar detection of analytes in blood

Science.gov (United States)

Joh, Daniel Y.; Hucknall, Angus M.; Wei, Qingshan; Mason, Kelly A.; Lund, Margaret L.; Fontes, Cassio M.; Hill, Ryan T.; Blair, Rebecca; Zimmers, Zackary; Achar, Rohan K.; Tseng, Derek; Gordan, Raluca; Freemark, Michael; Ozcan, Aydogan; Chilkoti, Ashutosh

2017-08-01

The ELISA is the mainstay for sensitive and quantitative detection of protein analytes. Despite its utility, ELISA is time-consuming, resource-intensive, and infrastructure-dependent, limiting its availability in resource-limited regions. Here, we describe a self-contained immunoassay platform (the “D4 assay”) that converts the sandwich immunoassay into a point-of-care test (POCT). The D4 assay is fabricated by inkjet printing assay reagents as microarrays on nanoscale polymer brushes on glass chips, so that all reagents are “on-chip,” and these chips show durable storage stability without cold storage. The D4 assay can interrogate multiple analytes from a drop of blood, is compatible with a smartphone detector, and displays analytical figures of merit that are comparable to standard laboratory-based ELISA in whole blood. These attributes of the D4 POCT have the potential to democratize access to high-performance immunoassays in resource-limited settings without sacrificing their performance.

The ELSA laser beamline for electron polarization measurements via Compton backscattering

Energy Technology Data Exchange (ETDEWEB)

Switka, Michael; Hinterkeuser, Florian; Koop, Rebecca; Hillert, Wolfgang [Electron Stretcher Facility ELSA, Physics Institute of Bonn University (Germany)

2016-07-01

The Electron Stretcher Facility ELSA provides a spin polarized electron beam with energies of 0.5 - 3.2 GeV for double polarization hadron physics experiments. As of 2015, the laser beamline of the polarimeter based on Compton backscattering restarted operation. It consists of a cw disk laser with design total beam power of 40 W and features two polarized 515 nm photon beams colliding head-on with the stored electron beam in ELSA. The polarization measurement is based on the vertical profile asymmetry of the back-scattered photons, which is dependent on the polarization degree of the stored electron beam. After recent laser repairs, beamline and detector modifications, the properties of the beamline have been determined and first measurements of the electron polarization degree were conducted. The beamline performance and first measurements are presented.

Species-Independent Modeling of High-Frequency Ultrasound Backscatter in Hyaline Cartilage.

Science.gov (United States)

Männicke, Nils; Schöne, Martin; Liukkonen, Jukka; Fachet, Dominik; Inkinen, Satu; Malo, Markus K; Oelze, Michael L; Töyräs, Juha; Jurvelin, Jukka S; Raum, Kay

2016-06-01

Apparent integrated backscatter (AIB) is a common ultrasound parameter used to assess cartilage matrix degeneration. However, the specific contributions of chondrocytes, proteoglycan and collagen to AIB remain unknown. To reveal these relationships, this work examined biopsies and cross sections of human, ovine and bovine cartilage with 40-MHz ultrasound biomicroscopy. Site-matched estimates of collagen concentration, proteoglycan concentration, collagen orientation and cell number density were employed in quasi-least-squares linear regression analyses to model AIB. A positive correlation (R(2) = 0.51, p 70°) to the sound beam direction. These findings indicate causal relationships between AIB and cartilage structural parameters and could aid in more sophisticated future interpretations of ultrasound backscatter. Copyright © 2016 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.

Characterization of Rayleigh backscattering arising in various two-mode fibers

NARCIS (Netherlands)

Yu, Dawei; Fu, Songnian; Cao, Zizheng; Tang, Ming; Deng, Lei; Liu, Deming; Giles, I.; Koonen, T.; Okonkwo, C.

2016-01-01

We experimentally characterize the mode dependent characteristics of Rayleigh backscattering (RB) arising in various two-mode fibers (TMFs). With the help of an all-fiber photonic lantern, we are able to measure the RB power at individual modes. Consequently, mode dependent power distribution of RB

Evaluation of field test kits including immunoassays for the detection of contaminants in soil and water

International Nuclear Information System (INIS)

Waters, L.C.; Smith, R.R.; Counts, R.W.; Stewart, J.H.; Jenkins, R.A.

1993-01-01

Effective field test methods are needed for hazardous waste site characterization and remediation. Useful field methods should be rapid, analyte-specific, cost-effective and accurate in the concentration range at which the analyte is regulated. In this study, field test kits for polychlorinated biphenyls (PCBs), mercury, lead and nitrate were evaluated with reference to these criteria. PCBs and mercury, in soils, were analyzed by immunoassay. Ionic lead and nitrate, in water, were measured chemically using test strips. Except for lead, each analyte was measured in both spiked and actual field samples. Twenty to 40 samples per day can be analyzed with the immunoassays and even more with the strip tests. The sensitivity of the immunoassays is in the 1-3 ppM range. Nitrate was consistently detected at ≥5 ppM; lead ions at ≥20 ppM. Results obtained using these methods compared favorably with those obtained by standard laboratory methods. In addition to being useful field screening methods, these kits can be used in the laboratory to sort out negative samples and/or to define proper dilutions for positive samples requiring further analysis

Homogeneous immunoassay for human IgG using oriented hen egg IgY immobilized on gold sol nanoparticles

International Nuclear Information System (INIS)

Yeritsyan, H.E.; Gasparyan, V.K.

2012-01-01

Homogeneous immunoassays using (red) gold nanoparticles represent an attractive detection scheme because of the option of photometric readout. We have applied oriented immobilization of hen egg immunoglobulin Y (IgY) on gold nanoparticles when developing a homogeneous immunoassay for human IgG. In oriented immobilization, as opposed to random immobilization, the antigen binding capabilities of the antibodies are retained. It is shown that such immunoassay has significantly better sensitivity in comparison with methods based on conventional immobilization of affinity-purified antibodies. It is also shown that hen egg IgY is better suited than rabbit antibodies, because much more antibody can be immobilized on gold nanoparticles without any destabilization, probably because of the more acidic nature of these antibodies. In addition, hen egg IgY can be supplied in higher quantity and can be prepared more easily than IgG from rabbits. Bleeding and slaughtering of animals is not needed. The assay presented here has a wide detection range (30-500 ng. mL -1 ) and a limit of detection as low as 30 ng. mL -1 of human IgG. (author)

The Harwell back-scattering spectrometer

International Nuclear Information System (INIS)

Windsor, C.G.; Bunce, L.J.; Borcherds, P.H.; Cole, I.; Fitzmaurice, M.; Johnson, D.A.G.; Sinclair, R.N.

1976-01-01

Neutron diffraction spectra in which both high resolution (Δ Q/Q approximately equal to 0.003) and high intensity are maintained up to scattering vectors as high as 30A -1 (sin theta/lambda = 2.5) have been obtained with the back-scattering spectrometer (BSS) recently installed on the Harwell electron linac. The theory behind the spectrometer design is described, and it is shown how the above resolution requirement leads to its basic features of a 12m incident flight path, a 2m scattering flight path and a scattering angle (2theta) acceptance from 165 0 to 175 0 . Examples of the resolution, intensity and background are given. It is shown that the problem of frame overlap may be overcome by using an absorbing filter. (author)

Analysis of dose and risk associated with the use of backscatter vans

International Nuclear Information System (INIS)

Correa, Samanda C.A.; Aquino, Josilto O.; Silva, Ademir X.

2011-01-01

In recent years, imaging technology using radiation has been gaining in importance for the screening of persons for security reasons and in order to detect contraband. And currently, new X-ray detection technologies have been introduced such as the Backscatter Van. The Backscatter Van is used in port and border security, force protection, urban surveillance, and other critical security applications in order to seize drugs, explosives, and more. The system is mobile, affordable and the faster, more effective, and less expensive than any mobile screening solution in the marketplace. However, although the application of this equipment in the national security area is indeed relevant, its use has caused a great deal of controversy, especially with regard to the doses absorbed and to the cancer induction risk associated with these exposures. The aim of this study is to use the Monte Carlo MCNPX code and the male adult voxel (MAX) and female adult voxel (FAX) phantoms, to evaluate the absorbed dose, effective dose and cancer induction risk values associated with the exposures of individuals submitted to Backscatter Van. The effective dose values were calculated as recommended by the new ICRP 103 and the cancer induction risk values were estimated through the BEIR VII document. (author)

Analysis of dose and risk associated with the use of backscatter vans

Energy Technology Data Exchange (ETDEWEB)

Correa, Samanda C.A.; Aquino, Josilto O., E-mail: scorrea@cnen.gov.b, E-mail: josilto@cnen.gov.b [Comissao Nacional de Energia Nuclear (DIAPI/CGMI/CNEN), Rio de Janeiro, RJ (Brazil). Coordenacao Geral de Instalacoes Medicas e Industriais. Div. de Aplicacoes Industriais; Souza, Edmilson M., E-mail: emonteiro@nuclear.ufrj.b [Centro Universitario Estadual da Zona Oeste (CAPI/UEZO), Rio de Janeiro, RJ (Brazil); Silva, Ademir X., E-mail: ademir@con.ufrj.b [Coordenacao dos Programas de Pos-Graduacao de Engenharia (PEN/COPPE/UFRJ), Rio de Janeiro, RJ (Brazil). Programa de Engenharia Nuclear

2011-07-01

In recent years, imaging technology using radiation has been gaining in importance for the screening of persons for security reasons and in order to detect contraband. And currently, new X-ray detection technologies have been introduced such as the Backscatter Van. The Backscatter Van is used in port and border security, force protection, urban surveillance, and other critical security applications in order to seize drugs, explosives, and more. The system is mobile, affordable and the faster, more effective, and less expensive than any mobile screening solution in the marketplace. However, although the application of this equipment in the national security area is indeed relevant, its use has caused a great deal of controversy, especially with regard to the doses absorbed and to the cancer induction risk associated with these exposures. The aim of this study is to use the Monte Carlo MCNPX code and the male adult voxel (MAX) and female adult voxel (FAX) phantoms, to evaluate the absorbed dose, effective dose and cancer induction risk values associated with the exposures of individuals submitted to Backscatter Van. The effective dose values were calculated as recommended by the new ICRP 103 and the cancer induction risk values were estimated through the BEIR VII document. (author)

Monte Carlo calculation of secondary electron emission from carbon-surface by obliquely incident particles

International Nuclear Information System (INIS)

Ohya, Kaoru; Kawata, Jun; Mori, Ichiro

1990-01-01

Incidence angle dependences of secondary electron emission from a carbon surface by low energy electron and hydrogen atom are calculated using Monte Carlo simulations on the kinetic emission model. The calculation shows very small increase or rather decrease of the secondary electron yield with oblique incidence. It is explained in terms of not only multiple elastic collisions of incident particles with the carbon atoms but also small penetration depth of the particles comparable with the escape depth of secondary electrons. In addition, the two types of secondary electron emission are distinguished by using the secondary electron yield statistics; one is the emission due to trapped particles in the carbon, and the other is that due to backscattered particles. The high-yield component of the statistics on oblique incidence is more suppressed than those on normal incidence. (author)

Imaging near-surface heterogeneities by natural migration of backscattered surface waves

KAUST Repository

AlTheyab, Abdullah; Lin, Fan-Chi; Schuster, Gerard T.

2016-01-01

We present a migration method that does not require a velocity model to migrate backscattered surface waves to their projected locations on the surface. This migration method, denoted as natural migration, uses recorded Green's functions along

NiCoBP-doped carbon nanotube hybrid: A novel oxidase mimetic system for highly efficient electrochemical immunoassay

Energy Technology Data Exchange (ETDEWEB)

Zhang, Bing; He, Yu; Liu, Bingqian; Tang, Dianping, E-mail: dianping.tang@fzu.edu.cn

2014-12-03

Highlights: • We report a new oxidase mimetic system for highly efficient electrochemical immunoassay. • NiCoBP-doped carbon nanotube hybrids were used as the nanocatalysts. • NiCoBP-doped carbon nanotube hybrids were used as the mimic oxidase. - Abstract: NiCoBP-doped multi-walled carbon nanotube (NiCoBP–MWCNT) was first synthesized by using induced electroless-plating method and functionalized with the biomolecules for highly efficient electrochemical immunoassay of prostate-specific antigen (PSA, used as a model analyte). We discovered that the as-synthesized NiCoBP–MWCNT had the ability to catalyze the glucose oxidization with a stable and well-defined redox peak. The catalytic current increased with the increment of the immobilized NiCoBP–MWCNT on the electrode. Transmission electron microscope (TEM) and energy dispersive X-ray spectrometry (EDX) were employed to characterize the as-prepared NiCoBP–MWCNT. Using the NiCoBP–MWCNT-conjugated anti-PSA antibody as the signal-transduction tag, a new enzyme-free electrochemical immunoassay protocol could be designed for the detection of target PSA on the capture antibody-functionalized immunosensing interface. Experimental results revealed that the designed immunoassay system could exhibit good electrochemical responses toward target PSA, and allowed the detection of PSA at a concentration as low as 0.035 ng mL{sup −1}. More importantly, the NiCoBP-MWCNT-based oxidase mimetic system could be further extended for the monitoring of other low-abundance proteins or disease-related biomarkers by tuning the target antibody.

Comparison of Ultrasound Attenuation and Backscatter Estimates in Layered Tissue-Mimicking Phantoms among Three Clinical Scanners

Science.gov (United States)

Nam, Kibo; Rosado-Mendez, Ivan M.; Wirtzfeld, Lauren A.; Ghoshal, Goutam; Pawlicki, Alexander D.; Madsen, Ernest L.; Lavarello, Roberto J.; Oelze, Michael L.; Zagzebski, James A.; O’Brien, William D.; Hall, Timothy J.

2013-01-01

Backscatter and attenuation coefficient estimates are needed in many quantitative ultrasound strategies. In clinical applications, these parameters may not be easily obtained because of variations in scattering by tissues overlying a region of interest (ROI). The goal of this study is to assess the accuracy of backscatter and attenuation estimates for regions distal to nonuniform layers of tissue-mimicking materials. In addition, this work compares results of these estimates for “layered” phantoms scanned using different clinical ultrasound machines. Two tissue-mimicking phantoms were constructed, each exhibiting depth-dependent variations in attenuation or backscatter. The phantoms were scanned with three ultrasound imaging systems, acquiring radio frequency echo data for offline analysis. The attenuation coefficient and the backscatter coefficient (BSC) for sections of the phantoms were estimated using the reference phantom method. Properties of each layer were also measured with laboratory techniques on test samples manufactured during the construction of the phantom. Estimates of the attenuation coefficient versus frequency slope, α0, using backscatter data from the different systems agreed to within 0.24 dB/cm-MHz. Bias in the α0 estimates varied with the location of the ROI. BSC estimates for phantom sections whose locations ranged from 0 to 7 cm from the transducer agreed among the different systems and with theoretical predictions, with a mean bias error of 1.01 dB over the used bandwidths. This study demonstrates that attenuation and BSCs can be accurately estimated in layered inhomogeneous media using pulse-echo data from clinical imaging systems. PMID:23160474

Rapid Salmonella detection in experimentally inoculated equine faecal and veterinary hospital environmental samples using commercially available lateral flow immunoassays.

Science.gov (United States)

Burgess, B A; Noyes, N R; Bolte, D S; Hyatt, D R; van Metre, D C; Morley, P S

2015-01-01

Salmonella enterica is the most commonly reported cause of outbreaks of nosocomial infections in large animal veterinary teaching hospitals and the closure of equine hospitals. Rapid detection may facilitate effective control practices in equine populations. Shipping and laboratory testing typically require ≥48 h to obtain results. Lateral flow immunoassays developed for use in food-safety microbiology provide an alternative that has not been evaluated for use with faeces or environmental samples. We aimed to identify enrichment methods that would allow commercially available rapid Salmonella detection systems (lateral flow immunoassays) to be used in clinical practice with equine faecal and environmental samples, providing test results in 18-24 h. In vitro experiment. Equine faecal and environmental samples were inoculated with known quantities of S. enterica serotype Typhimurium and cultured using 2 different enrichment techniques for faeces and 4 enrichment techniques for environmental samples. Samples were tested blindly using 2 different lateral flow immunoassays and plated on agar media for confirmatory testing. In general, commercial lateral flow immunoassays resulted in fewer false-negative test results with enrichment of 1 g faecal samples in tetrathionate for 18 h, while all environmental sample enrichment techniques resulted in similar detection rates. The limit of detection from spiked samples, ∼4 colony-forming units/g, was similar for all methods evaluated. The lateral flow immunoassays evaluated could reliably detect S. enterica within 18 h, indicating that they may be useful for rapid point-of-care testing in equine practice applications. Additional evaluation is needed using samples from naturally infected cases and the environment to gain an accurate estimate of test sensitivity and specificity and to substantiate further the true value of these tests in clinical practice. © 2014 EVJ Ltd.

Long Wavelength SAR Backscatter Modelling Trends as a Consequence of the Emergent Properties of Tree Populations

Directory of Open Access Journals (Sweden)

Matthew Brolly

2014-07-01

Full Text Available This study describes the novel use of a macroecological plant and forest structure model in conjunction with a Radiative Transfer (RT model to better understand interactions between microwaves and forest canopies. Trends predicted by the RT model, resulting from interactions with mixed age, mono and multi species forests, are analysed in comparison to those predicted using a simplistic structure based scattering model. This model relates backscatter to scatterer cross sectional or volume specifications, dependent on the size. The Spatially Explicit Reiterative Algorithm (SERA model is used to provide a widely varied tree size distribution while maintaining allometric consistency to produce a natural-like forest representation. The RT model is parameterised using structural information from SERA and microwave backscatter simulations are used to analyse the impact of changes to the forest stand. Results show that the slope of the saturation curve observed in the Synthetic Aperture Radar (SAR backscatter-biomass relationship is sensitive to thinning and therefore forest basal area. Due to similarities displayed between the results of the RT and simplistic model, it is determined that forest SAR backscatter behaviour at long microwave wavelengths may be described generally using equations related to total stem volume and basal area. The nature of these equations is such that they describe saturating behaviour of forests in the absence of attenuation in comparable fashion to the trends exhibited using the RT model. Both modelled backscatter trends predict a   relationship to forest basal area from an early age when forest volume is increasing. When this is not the case, it is assumed to be a result of attenuation of the dominant stem-ground interaction due to the presence of excessive numbers of stems. This work shows how forest growth models can be successfully incorporated into existing independent scattering models and reveals, through the RT

Tumor specific lung cancer diagnostics with multiplexed FRET immunoassays

Science.gov (United States)

Geißler, D.; Hill, D.; Löhmannsröben, H.-G.; Thomas, E.; Lavigne, A.; Darbouret, B.; Bois, E.; Charbonnière, L. J.; Ziessel, R. F.; Hildebrandt, N.

2010-02-01

An optical multiplexed homogeneous (liquid phase) immunoassay based on FRET from a terbium complex to eight different fluorescent dyes is presented. We achieved highly sensitive parallel detection of four different lung cancer specific tumor markers (CEA, NSE, SCC and CYFRA21-1) within a single assay and show a proof-of-principle for 5- fold multiplexing. The method is well suited for fast and low-cost miniaturized point-of-care testing as well as for highthroughput screening in a broad range of in-vitro diagnostic applications.

[Particle Size and Number Density Online Analysis for Particle Suspension with Polarization-Differentiation Elastic Light Scattering Spectroscopy].

Science.gov (United States)

Chen, Wei-kang; Fang, Hui

2016-03-01

The basic principle of polarization-differentiation elastic light scattering spectroscopy based techniques is that under the linear polarized light incidence, the singlely scattered light from the superficial biological tissue and diffusively scattered light from the deep tissue can be separated according to the difference of polarization characteristics. The novel point of the paper is to apply this method to the detection of particle suspension and, to realize the simultaneous measurement of its particle size and number density in its natural status. We design and build a coaxial cage optical system, and measure the backscatter signal at a specified angle from a polystyrene microsphere suspension. By controlling the polarization direction of incident light with a linear polarizer and adjusting the polarization direction of collected light with another linear polarizer, we obtain the parallel polarized elastic light scattering spectrum and cross polarized elastic light scattering spectrum. The difference between the two is the differential polarized elastic light scattering spectrum which include only the single scattering information of the particles. We thus compare this spectrum to the Mie scattering calculation and extract the particle size. We then also analyze the cross polarized elastic light scattering spectrum by applying the particle size already extracted. The analysis is based on the approximate expressions taking account of light diffusing, from which we are able to obtain the number density of the particle suspension. We compare our experimental outcomes with the manufacturer-provided values and further analyze the influence of the particle diameter standard deviation on the number density extraction, by which we finally verify the experimental method. The potential applications of the method include the on-line particle quality monitoring for particle manufacture as well as the fat and protein density detection of milk products.

Proceedings of the international advisory committee on 'biomolecular dynamics instrument DNA' and the workshop on 'biomolecular dynamics backscattering spectrometers'

International Nuclear Information System (INIS)

Arai, Masatoshi; Aizawa, Kazuya; Nakajima, Kenji; Shibata, Kaoru; Takahashi, Nobuaki

2008-08-01

A workshop entitled 'Biomolecular Dynamics Backscattering Spectrometers' was held on February 27th - 29th, 2008 at J-PARC Center, Japan Atomic Energy Agency. This workshop was planned to be held for aiming to realize an innovative neutron backscattering instrument, namely DNA, in the MLF and thus four leading scientists in the field of neutron backscattering instruments were invited as the International Advisory Committee (IAC member: Dr. Dan Neumann (Chair); Prof. Ferenc Mezei; Dr. Hannu Mutka; Dr. Philip Tregenna-Piggott) for DNA from institutes in the United States, France and Switzerland, where backscattering instruments are in-service. It was therefore held in the form of lecture anterior and then in the form of the committee posterior. This report includes the executive summary of the IAC and materials of the presentations in the IAC and the workshop. (author)

Frequency shift of the Bragg and Non-Bragg backscattering from periodic water wave

Science.gov (United States)

Wen, Biyang; Li, Ke

2016-08-01

Doppler effect is used to measure the relative speed of a moving target with respect to the radar, and is also used to interpret the frequency shift of the backscattering from the ocean wave according to the water-wave phase velocity. The widely known relationship between the Doppler shift and the water-wave phase velocity was deduced from the scattering measurements data collected from actual sea surface, and has not been verified under man-made conditions. Here we show that this ob- served frequency shift of the scattering data from the Bragg and Non-Bragg water wave is not the Doppler shift corresponding to the water-wave phase velocity as commonly believed, but is the water-wave frequency and its integral multiple frequency. The power spectrum of the backscatter from the periodic water wave consists of serials discrete peaks, which is equally spaced by water wave frequency. Only when the water-wave length is the integer multiples of the Bragg wave, and the radar range resolution is infinite, does the frequency shift of the backscattering mathematically equal the Doppler shift according to the water-wave phase velocity.

Estimating Snow Water Equivalent with Backscattering at X and Ku Band Based on Absorption Loss

Directory of Open Access Journals (Sweden)

Yurong Cui

2016-06-01

Full Text Available Snow water equivalent (SWE is a key parameter in the Earth’s energy budget and water cycle. It has been demonstrated that SWE can be retrieved using active microwave remote sensing from space. This necessitates the development of forward models that are capable of simulating the interactions of microwaves and the snow medium. Several proposed models have described snow as a collection of sphere- or ellipsoid-shaped ice particles embedded in air, while the microstructure of snow is, in reality, more complex. Natural snow usually forms a sintered structure following mechanical and thermal metamorphism processes. In this research, the bi-continuous vector radiative transfer (bi-continuous-VRT model, which firstly constructs snow microstructure more similar to real snow and then simulates the snow backscattering signal, is used as the forward model for SWE estimation. Based on this forward model, a parameterization scheme of snow volume backscattering is proposed. A relationship between snow optical thickness and single scattering albedo at X and Ku bands is established by analyzing the database generated from the bi-continuous-VRT model. A cost function with constraints is used to solve effective albedo and optical thickness, while the absorption part of optical thickness is obtained from these two parameters. SWE is estimated after a correction for physical temperature. The estimated SWE is correlated with the measured SWE with an acceptable accuracy. Validation against two-year measurements, using the SnowScat instrument from the Nordic Snow Radar Experiment (NoSREx, shows that the estimated SWE using the presented algorithm has a root mean square error (RMSE of 16.59 mm for the winter of 2009–2010 and 19.70 mm for the winter of 2010–2011.

Use of GSR particle analysis program on an analytical SEM to identify sources of emission of airborne particles

International Nuclear Information System (INIS)

Chan, Y.C.; Trumper, J.; Bostrom, T.

2002-01-01

Full text: High concentrations of airborne particles, in particular PM 10 (particulate matter 10 , but has been little used in Australia for airborne particulates. Two sets of 15 mm PM 10 samples were collected in March and April 2000 from two sites in Brisbane, one within a suburb and one next to an arterial road. The particles were collected directly onto double-sided carbon tapes with a cascade impactor attached to a high-volume PM 10 sampler. The carbon tapes were analysed in a JEOL 840 SEM equipped with a Be-window energy-dispersive X-ray detector and Moran Scientific microanalysis system. An automated Gun Shot Residue (GSR) program was used together with backscattered electron imaging to characterise and analyse individual particulates. About 6,000 particles in total were analysed for each set of impactor samples. Due to limitations of useful pixel size, only particles larger than about 0.5 μm could be analysed. The size, shape and estimated elemental composition (from Na to Pb) of the particles were subjected to non-hierarchical cluster analysis and the characteristics of the clusters were related to their possible sources of emission. Both samples resulted in similar particle clusters. The particles could be classified into three main categories non-spherical (58% of the total number of analysed particles, shape factor >1 1), spherical (15%) and 'carbonaceous' (27%, ie with unexplained % of elemental mass >75%). Non-spherical particles were mainly sea salt and soil particles, and a small amount of iron, lead and mineral dust. The spherical particles were mainly sea salt particles and flyash, and a small amount of iron, lead and secondary sulphate dust. The carbonaceous particles included carbon material mixed with secondary aerosols, roadside dust, sea salt or industrial dust. The arterial road sample also contained more roadside dust and less secondary aerosols than the suburb sample. Current limitations with this method are the minimum particle size

A Laboratory Experiment on EM Backscatter from Farley-Buneman and Gradient Drift Waves

DEFF Research Database (Denmark)

Alport, M. J.; D'Angelo, N.; Pécseli, Hans

1981-01-01

Results are reported of a laboratory experiment on Bragg backscatter of 3-cm microwaves by turbulent waves driven by the Farley-Buneman and gradient drift instabilities. The present work is the third in a series of laboratory experiments performed to test, under controlled conditions, prevalent i...... ideas on EM scattering by equatorial and high-latitude ionospheric waves and irregularities.......Results are reported of a laboratory experiment on Bragg backscatter of 3-cm microwaves by turbulent waves driven by the Farley-Buneman and gradient drift instabilities. The present work is the third in a series of laboratory experiments performed to test, under controlled conditions, prevalent...

Magnetofluorescent nanocomposites and quantum dots used for optimal application in magnetic fluorescence-linked immunoassay.

Science.gov (United States)

Tsai, H Y; Li, S Y; Fuh, C Bor

2018-03-01

Magnetofluorescent nanocomposites with optimal magnetic and fluorescent properties were prepared and characterized by combining magnetic nanoparticles (iron oxide@polymethyl methacrylate) with fluorescent nanoparticles (rhodamine 6G@mSiO 2 ). Experimental parameters were optimized to produce nanocomposites with high magnetic susceptibility and fluorescence intensity. The detection of a model biomarker (alpha-fetoprotein) was used to demonstrate the feasibility of applying the magnetofluorescent nanocomposites combined with quantum dots and using magnetic fluorescence-linked immunoassay. The magnetofluorescent nanocomposites enable efficient mixing, fast re-concentration, and nanoparticle quantization for optimal reactions. Biofunctional quantum dots were used to confirm the alpha-fetoprotein (AFP) content in sandwich immunoassay after mixing and washing. The analysis time was only one third that required in ELISA. The detection limit was 0.2 pg mL -1 , and the linear range was 0.68 pg mL -1 -6.8 ng mL -1 . This detection limit is lower, and the linear range is wider than those of ELISA and other methods. The measurements made using the proposed method differed by less than 13% from those obtained using ELISA for four AFP concentrations (0.03, 0.15, 0.75, and 3.75 ng mL -1 ). The proposed method has a considerable potential for biomarker detection in various analytical and biomedical applications. Graphical abstract Magnetofluorescent nanocomposites combined with fluorescent quantum dots were used in magnetic fluorescence-linked immunoassay.

Implementation of a near backscattering imaging system on the National Ignition Facility

International Nuclear Information System (INIS)

Mackinnon, A.J.; McCarville, T.; Piston, K.; Niemann, C.; Jones, G.; Reinbachs, I.; Costa, R.; Celeste, J.; Holtmeier, G.; Griffith, R.; Kirkwood, R.; MacGowan, B.; Glenzer, S.H.; Latta, M.R.

2004-01-01

A near backscattering imaging diagnostic system is being implemented on the first quad of beams on the National Ignition Facility. This diagnostic images diffusing scatter plates, placed around the final focus lenses on the National Ignition Facility target chamber, to quantitatively measure the fraction of light backscattered outside of the focusing cone angle of incident laser beam. A wide-angle imaging system relays an image of light scattered outside the lens onto a gated charge coupled device camera, providing 3 mm resolution over a 2 m field of view. To account for changes of the system throughput due to exposure to target debris the system will be routinely calibrated in situ at 532 and 355 nm using a dedicated pulsed laser source