WorldWideScience

Sample records for bacillus-like organisms isolated

  1. Isolation and characterization of organisms growing with methane only

    International Nuclear Information System (INIS)

    More than 20 obligate methane utilizing bacteria were isolated from local soil following shake flask enrichment and continuous subculture technique isolation of methanotrophs proved to the difficult and slow. All isolates were gram negative, strictly aerobic, use methane as their sole carbon and energy source, unable to grow on nutrient media and can use nitrate as N source. Some isolates were motile. Few were able to utilize methanol. All the tested soil samples contained methane utilizing organisms. (author)

  2. Draft Genome Sequence of Bacillus mesonae FJAT-13985T (=DSM 25968T) for Setting Up Phylogenomics in Genomic Taxonomy of the Bacillus-Like Bacteria

    Science.gov (United States)

    Liu, Guo-hong; Zhu, Yu-jing; Wang, Jie-ping; Che, Jian-mei; Chen, Qian-qian; Chen, Zheng

    2016-01-01

    Bacillus mesonae FJAT-13985T is a Gram-positive, spore-forming, and aerobic bacterium. Here, we report the draft genome sequence of B. mesonae FJAT-13985T with 5,807,726 bp, which will provide useful information for setting up phylogenomics in the genomic taxonomy of the Bacillus-like bacteria, as well as for the functional gene mining and application of B. mesonae FJAT-13985T. PMID:27313309

  3. Directional charge separation in isolated organic semiconductor crystalline nanowires

    OpenAIRE

    Labastide, J. A.; Thompson, H. B.; Marques, S.R.; Colella, N. S.; Briseno, A. L.; M.D. Barnes

    2016-01-01

    One of the fundamental design paradigms in organic photovoltaic device engineering is based on the idea that charge separation is an extrinsically driven process requiring an interface for exciton fission. This idea has driven an enormous materials science engineering effort focused on construction of domain sizes commensurate with a nominal exciton diffusion length of order 10 nm. Here, we show that polarized optical excitation of isolated pristine crystalline nanowires of a small molecule n...

  4. Directional charge separation in isolated organic semiconductor crystalline nanowires

    Science.gov (United States)

    Labastide, J. A.; Thompson, H. B.; Marques, S. R.; Colella, N. S.; Briseno, A. L.; Barnes, M. D.

    2016-02-01

    One of the fundamental design paradigms in organic photovoltaic device engineering is based on the idea that charge separation is an extrinsically driven process requiring an interface for exciton fission. This idea has driven an enormous materials science engineering effort focused on construction of domain sizes commensurate with a nominal exciton diffusion length of order 10 nm. Here, we show that polarized optical excitation of isolated pristine crystalline nanowires of a small molecule n-type organic semiconductor, 7,8,15,16-tetraazaterrylene, generates a significant population of charge-separated polaron pairs along the π-stacking direction. Charge separation was signalled by pronounced power-law photoluminescence decay polarized along the same axis. In the transverse direction, we observed exponential decay associated with excitons localized on individual monomers. We propose that this effect derives from an intrinsic directional charge-transfer interaction that can ultimately be programmed by molecular packing geometry.

  5. Directional charge separation in isolated organic semiconductor crystalline nanowires.

    Science.gov (United States)

    Labastide, J A; Thompson, H B; Marques, S R; Colella, N S; Briseno, A L; Barnes, M D

    2016-01-01

    One of the fundamental design paradigms in organic photovoltaic device engineering is based on the idea that charge separation is an extrinsically driven process requiring an interface for exciton fission. This idea has driven an enormous materials science engineering effort focused on construction of domain sizes commensurate with a nominal exciton diffusion length of order 10 nm. Here, we show that polarized optical excitation of isolated pristine crystalline nanowires of a small molecule n-type organic semiconductor, 7,8,15,16-tetraazaterrylene, generates a significant population of charge-separated polaron pairs along the π-stacking direction. Charge separation was signalled by pronounced power-law photoluminescence decay polarized along the same axis. In the transverse direction, we observed exponential decay associated with excitons localized on individual monomers. We propose that this effect derives from an intrinsic directional charge-transfer interaction that can ultimately be programmed by molecular packing geometry. PMID:26912040

  6. Simple method of isolating humic acids from organic soils

    Science.gov (United States)

    Ahmed, O. H.; Susilawati, K.; Nik Muhamad, A. B.; Khanif, M. Y.

    2009-04-01

    Humic substances particularly humic acids (HA) play a major role in soil conditioning e.g. erosion control, soil cation exchange capacity, complexation of heavy metal ions and pesticides, carbon and nitrogen cycles, plant growth and reduction of ammonia volatilization from urea. Humified substances such as coal, composts, and peat soils have substantial amounts of HA but the isolation of these acids is expensive, laborious, and time consuming. Factors that affect the quality and yield of HA isolated from these materials include extraction, fractionation, and purification periods. This work developed a simple, rapid, and cost effective method of isolating HA from peat soils. There was a quadratic relationship between extraction period and HA yield. Optimum extraction period was estimated at 4 h instead of the usual range of 12 to 48 h. There was no relationship between fractionation period and HA yield. As such 2 h instead of the usual range of 12 to 24 h fractionation period could be considered optimum. Low ash content (5%), remarkable reduction in K, coupled with the fact that organic C, E4/E6, carboxylic COOH, phenolic OH, and total acidity values of the HA were consistent with those reported by other authors suggest that the HA dealt with were free from mineral matter. This was possible because the distilled water used to purify the HA served as Bronsted-Lowry acid during the purification process of the HA. Optimum purification period using distilled waster was 1 h instead of the usual range of 1 and 7 days (uses HF and HCl and dialysis). Humic acids could be isolated from tropical peat soils within 7 h (i.e. 4 h extraction, 2 h fractionation, and 1 h purification) instead of the existing period of 2 and 7 days. This could facilitate the idea of producing organic fertilizers such as ammonium-humate and potassium-humate from humified substances since techniques devised in this study did not alter the true nature of the HA. Besides, the technique is rapid, simple

  7. Radiation-resistant micro-organisms isolated from textiles

    International Nuclear Information System (INIS)

    Towels from private homes and public offices and underwear contaminated by being used by employees at a public health laboratory were examined for occurrence of radiation-resistant bacteria and fungi. Three different methods were used for isolation of the most resistant organisms, one with multiplication of the microbial population prior to an irradiation used for selection, and two witout this multiplication and with the organisms placed on membrane filters or in situ on the textiles, respectively. A total of 44 different strains were isolated. Differences in the three methods used for selection of the most radiation-resistant microorganisms were not reflected in the results. 16 pigmentproducing Gram-positive cocci, tentatively classified as Micrococcus radiodurans, were the most radiation-resistant and were isolated in about half of the examinations. Other Gram-positive cocci, nonspore forming rods, some Nocardia and Candida parapsilosis strains and two Bacillus strains constituted the rest of the collection. With few exceptions dose-response curves for the strains were upward convex. D-6 values determined to be between 1.5 megarad for the most radiation sensitive, a Candida, and 5.7 megarad for the most resistant, tentatively classified as M. radiodurans. The D-6 values for the Bacillus strains were in both cases 1.8 megarad, consistent with a D-value of 0.3 megarad. The same resistance is reported to be the maximum resistance for B. pumilus, strain E601, commonly used as reference strain in the literature on radiation sterilization of medical devices and supplies. (author)

  8. Isolating factors that determine the organic enrichment of sea spray

    Science.gov (United States)

    Gantt, B.; Morris, E.; Petters, M. D.; Meskhidze, N.

    2010-12-01

    the sea spray. We calculated organic aerosol enrichment, aerosol number emissions, and CCN number at 0.2% supersaturation as a function of bubble size (~200-1000 µm) and organic compound concentration (mass fractions 10-4 - 10-1 relative to sea salt). Our results indicate that organic film thickness at the artificial seawater surface and the bubble size play major roles in determining the organic enrichment and hygroscopicity of the aerosols formed in the experimental chamber. Decreasing the bubble diameter from 1000 to 200 µm decreased the κ-value of the emitted aerosols from ~1.2 to ~0.6. A κ-value of 0.6 suggests that the aerosol is composed of ~50% organic by volume, a fraction many orders of magnitude greater than organic compound-to-sea salt ratio in the artificial sea water. An organic film thickness > ~1µm at the surface decreases κ-values to 50 µm. This implies that despite the fact that a large number of particles are being emitted above the solution, a 50 µm thick film over the water reduced the number emission of aerosols capable of serving as CCN (at 0.2% super saturation) to near 0. Analysis of our results suggest that in current laboratory studies we were able to isolate several factors controlling organic enrichment and CCN formation potential of sea spray.

  9. Isolation and characterization of organic-sulfur degradation bacterial strain

    Institute of Scientific and Technical Information of China (English)

    YANG Yu; DIAO Meng-xue; SHI Wu-yang; LI Li; DAI Qin-yun; QIU Guan-zhou

    2007-01-01

    A bacterial strain that was capable of degrading organic sulfur (dibenzothiophene) was isolated by enrichment techniques from the petroleum-contaminated soil collected from Zhongyuan Oil Field. The strain is named ZYX and is gram-positive.This strain undergoes bacilus-coccus morphological change, and forms yellow-pigment glossy circular colonies with 1.5 mm in diameter on average after 2 d incubation on Luria-Bertani(LB) plates. The full-length of 16S rDNA sequence of strain ZYX was determined and analyzed. Strain ZYX is found most relative with the genus of Arthrobacter. The similarity values between ZYX and Arthrobacter sp. P2 is 99.53%. The main morphological, biochemical and physiological features of strain ZYX accord with those of Arthrobacter. It is found that the optimal initial pH for growth is about 7.0, and the optimal concentration of dibenzothiophene(DBT)for growth is 0.10 g/L. Additionally, the results show that the best carbon source and nitrogen source are glycerol and glutamine,respectively.

  10. Photobacterium swingsii sp. nov., isolated from marine organisms.

    Science.gov (United States)

    Gomez-Gil, Bruno; Roque, Ana; Rotllant, Guiomar; Peinado, Lauro; Romalde, Jesus L; Doce, Alejandra; Cabanillas-Beltrán, Hector; Chimetto, Luciane A; Thompson, Fabiano L

    2011-02-01

    Six Gram-negative coccobacilli were isolated from Pacific oysters (Crassostrea gigas) from Mexico and haemolymph of spider crabs (Maja brachydactyla) from Spain. All of the isolates grew as small green colonies on thiosulphate-citrate-bile salts-sucrose (TCBS) agar and were facultatively anaerobic, oxidase-positive and sensitive to the vibriostatic agent O/129. Repetitive palindromic PCR analysis revealed a high degree of genomic homogeneity among the isolates. Several phenotypic traits differentiated the isolates from the type strains of species of the genus Photobacterium. DNA-DNA relatedness between two representative isolates and their closest phylogenetic neighbours by 16S rRNA gene sequence similarity, Photobacterium aplysiae CAIM 14(T) and Photobacterium frigidiphilum CAIM 20(T), was 44.01-53.85 %. We propose a novel species of the genus Photobacterium to accommodate the six isolates, with the name Photobacterium swingsii sp. nov. The type strain is CAIM 1393(T) (=CECT 7576(T)). PMID:20228205

  11. Biological control of chestnut canker, caused by Cryphonectria parasitica, by antagonistic organisms and hypovirulent isolates

    OpenAIRE

    AKILLI, Seçil; KATIRCIOĞLU, Yakup Zekai; MADEN, Salih

    2011-01-01

    Biological control of chestnut blight was investigated by using 3 hypovirulent isolates of Cryphonectria parasitica, 5 Trichoderma sp., 4 Penicillium sp., and 4 Bacillus sp. isolates. Hypovirulent isolates and antagonistic organisms were obtained from samples collected from the Black Sea region of Turkey, in 2008 and 2009. Effectiveness of the hypovirulent isolates and antagonistic microorganisms was tested on 3-year-old chestnut saplings. In the tests, bark disks of 6 mm were removed from th...

  12. Prevalence and characterization of antimicrobial-resistant Escherichia coli isolated from conventional and organic vegetables.

    Science.gov (United States)

    Kim, Sara; Woo, Gun-Jo

    2014-10-01

    To compare the characteristics and to identify the epidemiological relationships of Escherichia coli isolated from organic and conventional vegetables, the antimicrobial resistance and genetic properties of E. coli were investigated from 2010 to 2011. E. coli was isolated from 1 of 111 (0.9%) organic vegetables and from 20 of 225 (8.9%) conventional vegetables. The majority of strains were isolated from the surrounding farming environment (n=27/150 vs. 49/97 in organic vs. conventional samples). The majority of the vegetable strains were isolated from the surrounding farming environments. E. coli isolated from organic vegetables showed very low antimicrobial resistance rates except for cephalothin, ranging from 0% to 17.9%, while the resistance rates to cephalothin (71%) were extremely high in both groups. E. coli isolates expressed various resistance genes, which most commonly included blaTEM, tet(A), strA, strB, and qnrS. However, none of the isolates harbored tet(D), tet(E), tet(K), tet(L), tet(M), or qnrA. The transferability of tet gene, tet(A), and tet(B) was identified in tetracycline-resistant E. coli, and the genetic relationship was confirmed in a few cases from different sources. With regard to the lower antimicrobial resistance found in organic produce, this production mode seems able to considerably reduce the selection of antimicrobial-resistant bacteria on vegetables. PMID:25140978

  13. Organic fragments from graphene oxide: Isolation, characterization and solvent effects

    Indian Academy of Sciences (India)

    Ravula Thirupathi; Y Jayasubba Reddy; Erode N Prabhakaran; Hanudatta S Atreya

    2014-05-01

    As-prepared graphene oxide (GO) contains oxidative debris which can be washed using basic solutions. We present the isolation and characterization of these debris. Dynamic light scattering (DLS) is used to monitor the separation of the debris in various solvents in the presence of different protic and aprotic alkylamino bases. The study reveals that the debris are rich in carbonyl functional groups and water is an essential component for separation and removal of the debris from GO under oxidative reaction conditions.

  14. Sterols of Pneumocystis carinii hominis organisms isolated from human lungs

    DEFF Research Database (Denmark)

    Kaneshiro, E S; Amit, Z; Chandra, Jan Suresh; Baughman, R P; Contini, C; Lundgren, B

    1999-01-01

    The opportunistic pathogen Pneumocystis carinii causes pneumonia (P. carinii pneumonia, or PCP) in immunocompromised individuals such as AIDS patients. Rat-derived P. carinii carinii organisms have distinct sterols which are not synthesized by mammals and not found in other microbes infecting mam...

  15. An Approach for Isolated Testing of Self-Organization Algorithms

    OpenAIRE

    Eberhardinger, Benedikt; Anders, Gerrit; Seebach, Hella; Siefert, Florian; Knapp, Alexander; Reif, Wolfgang

    2016-01-01

    We provide a systematic approach for testing self-organization (SO) algorithms. The main challenges for such a testing domain are the strongly ramified state space, the possible error masking, the interleaving of mechanisms, and the oracle problem resulting from the main characteristics of SO algorithms: their inherent non-deterministic behavior on the one hand, and their dynamic environment on the other. A key to success for our SO algorithm testing framework is automation, since it is rarel...

  16. Antifouling Activity of Secondary Metabolites Isolated from Chinese Marine Organisms

    KAUST Repository

    Li, Yong Xin

    2013-04-25

    Biofouling results in tremendous economic losses to maritime industries around the world. A recent global ban on the use of organotin compounds as antifouling agents has further raised demand for safe and effective antifouling compounds. In this study, 49 secondary metabolites, including diterpenoids, steroids, and polyketides, were isolated from soft corals, gorgonians, brown algae, and fungi collected along the coast of China, and their antifouling activity was tested against cyprids of the barnacle Balanus (Amphibalanus) amphitrite. Twenty of the compounds were found to inhibit larval settlement significantly at a concentration of 25 μg ml-1. Two briarane diterpenoids, juncin O (2) and juncenolide H (3), were the most promising non-toxic antilarval settlement candidates, with EC50 values less than 0.13 μg ml-1 and a safety ratio (LC50/EC50) higher than 400. A preliminary structure-activity relationships study indicated that both furanon and furan moieties are important for antifouling activity. Intriguingly, the presence of hydroxyls enhanced their antisettlement activity. © 2013 Springer Science+Business Media New York.

  17. Fluorescence Characterization of Dissolved Organic Matter Isolates from Sediments and the Association with Phenanthrene Binding Affinity

    Science.gov (United States)

    Hur, Jin; Lee, Bo-Mi; Shin, Kyung-Hoon

    2014-05-01

    In this study, selected spectroscopic characteristics of sediment organic matter (SOM) were compared and discussed with respect to their different isolation methods, the source discrimination capabilities, and the association with the extent of phenanthrene binding. A total of 16 sediments were collected from three categorized locations including a costal lake, industrial areas, and the upper streams, each of which is likely influenced by the organic sources of algal production, industrial effluent, and terrestrial input, respectively. The spectroscopic properties related to aromatic structures and terrestrial humic acids were more pronounced for alkaline extractable organic matter (AEOM) isolates than for the SOM isolates based on water soluble extracts and porewater. The three categorized sampling locations were the most differentiated in the AEOM isolates, suggesting AEOM may be the most representative SOM isolates in describing the chemical properties and the organic sources of SOM. Parallel factor analysis (PARAFAC) based on fluorescence excitation-emission matrix (EEM) showed that a combination of four fluorescent groups could represent all the fluorescence features of SOM. The three categorized sampling locations were well discriminated by the percent distributions of terrestrial and microbial humic-like fluorescent groups of the AEOM isolates. The relative distribution of terrestrial humic-like fluorophores was highly correlated with the extent of phenanthrene binding (r=0.676; phumic acids in SOM may contribute to the enhancement of binding with hydrophobic organic contaminants in sediments. Principal component analysis (PCA) further demonstrated that the extent of SOM's binding affinity might be affected by the degree of biological transformation in SOM as well as the abundance of aromatic carbon structures.

  18. Genome Sequences of Three Spore-Forming Bacteria Isolated from the Feces of Organically Raised Chickens

    Science.gov (United States)

    Kennedy, Victoria; Van Laar, Tricia A.; Aleru, Omoshola; Thomas, Michael; Ganci, Michelle

    2016-01-01

    Antibiotic feed supplements have been implicated in the rise of multidrug-resistant bacteria. An alternative to antibiotics is probiotics. Here, we report the genome sequences of two Bacillus and one Solibacillus species, all spore-forming, Gram-positive bacteria, isolated from the feces organically raised chicken feces, with potential to serve as probiotics. PMID:27587809

  19. Genome Sequences of Three Spore-Forming Bacteria Isolated from the Feces of Organically Raised Chickens.

    Science.gov (United States)

    Kennedy, Victoria; Van Laar, Tricia A; Aleru, Omoshola; Thomas, Michael; Ganci, Michelle; Rawat, Mamta

    2016-01-01

    Antibiotic feed supplements have been implicated in the rise of multidrug-resistant bacteria. An alternative to antibiotics is probiotics. Here, we report the genome sequences of two Bacillus and one Solibacillus species, all spore-forming, Gram-positive bacteria, isolated from the feces organically raised chicken feces, with potential to serve as probiotics. PMID:27587809

  20. Draft Genome Sequence of a New Zealand Rickettsia-Like Organism Isolated from Farmed Chinook Salmon

    Science.gov (United States)

    Draper, Jenny; Brosnahan, Cara L.; Orr, Della; McFadden, Andrew; Jones, Brian

    2016-01-01

    We report here the draft genome sequence of a rickettsia-like organism, isolated from a New Zealand Chinook salmon farm experiencing high mortality. The genome is approximately 3 Mb in size, has a G+C content of approximately 39.2%, and is predicted to contain 2,870 coding sequences. PMID:27365345

  1. Isolation studies on the prevalance of salmonellae in chicken organs, eggs and feed components

    International Nuclear Information System (INIS)

    Background: Salmonella is an important zoonotic pathogen and its prevalence in the chicken meat and eggs acts as a continuous threat to human population. The current studies covering a time period of three years, was carried out to report the isolation of salmonellae from the chicken tissues, eggs and feed ingredient. Methods: A total of 1747 random samples from twelve different sources and 56 locations in Islamabad and Northern Punjab area of Pakistan, were screened for isolation studies according to the already published established protocols. Results: The analysis of 1747 random samples comprising of 1069 (61.19%) chicken organs and 678 (38.81%) allied sources including eggs and feed ingredients, showed that a total of 162 (9.27%) were positive for salmonellae. Isolation prevalence in various chicken organs and allied sources was 86 (8.04%) and 76 (11.20%) respectively. The maximum isolation prevalence was recorded in meat meal (19.35%), followed by fish meal (17.54%), hatchery fluff (14.63%), livers (13.17%), poultry litter (10.89%), and eggs (9.64%). The range of Salmonella isolated varied from 19.35% to 4.72% in various organs and allied sources. Conclusions: Our findings highlighted a potential public health hazard and emphasized the significance of continuous surveillance system in the country to understand the ever changing epidemiological pattern of Salmonella serovers. The endemic prevalence of various serovars can cause outbreaks of human salmonellosis due to the consumption of contaminated meat and eggs as has already been reported worldwide. (author)

  2. Antimicrobial Effect of Bee Honey in Comparison to Antibiotics on Organisms Isolated From Infected Burns

    OpenAIRE

    Abd-El Aal, A.M.; El-Hadidy, M.R.; El-Mashad, N.B.; El-Sebaie, A.H.

    2007-01-01

    Despite recent advances in antimicrobial chemotherapy and burn wound management, infection continues to be an important problem in burns. Honey is the most famous rediscovered remedy that is used to treat infected wounds and promote healing. The present study aims to evaluate the antimicrobial effect of bee honey on organisms isolated from infected burns in comparison to the antibiotics used in treatment of burn infection, and to evaluate the effects produced when bee honey is added to antibi...

  3. Impact of Isolated Coronary Artery Bypass Grafting on Non-Organic Tricuspid Regurgitation Severity

    Directory of Open Access Journals (Sweden)

    Mahmood Sheikhfathollahi

    2009-12-01

    Full Text Available Background: Moderate non-organic tricuspid regurgitation (TR concomitant with coronary artery disease is not uncommon. Whether or not TR improves after pure coronary artery bypass grafting (CABG, however, is unclear. The aim of this study was to evaluate the effect of isolated CABG on moderate non-organic TR.Methods: This study recruited 50 patients (40% female, mean age: 65.38±8.01 years, mean left ventricular ejection fraction (LVEF: 45.74±13.05% with moderate non-organic TR who underwent isolated CABG. TR severity before and after CABG was compared. Pulmonary arterial systolic pressure (PAPs>30mmHg and LVEF<50% were considered elevated PAPs (EPAPs and LV systolic dysfunction, respectively. Presence of Q-wave in leads II, III, and aVF was considered inferior myocardial infarction (inf. MI.Results: Pre-operatively, 81.5% of the patients had EPAPs, 16% right ventricle (RV dilation, and 50% left ventricle (LV and 16% RV systolic dysfunction. TR severity improved in 64% after CABG, whereas it remained unchanged or even worsened in others (P value<0.001. Patients with inf. MI showed no improvement in TR, while patients without inf. MI had significant TR regression after CABG (P value=0.050. Improvement of TR severity after CABG was not related to pre-operative RV size and function, LV systolic function, or PAPs reduction.Conclusion: Although TR severity decreased remarkably after isolated CABG, a considerable number of the patients had no TR regression. In addition, only absence of inf. MI was significantly correlated to TR improvement after CABG. Further prospective studies with long-term follow-up are needed to determine the other factors predicting TR regression after isolated CABG.

  4. A Comparative Study on Probiotic Organisms Isolated from Different food and Milk Products and Medicinal Capsules

    Directory of Open Access Journals (Sweden)

    Raksha L Bayas

    2014-06-01

    Full Text Available A number of health benefits have been claimed for probiotic bacteria such as Lactobacillus species and Enterococcus species. Beneficial effects of food, milk product and medicinal capsules with live microbes (probiotics on human health are being increasingly promoted by professionals. These benefits include antimicrobial, anti-mutagenic effects, anti-carcinogenic properties, improvement in lactose metabolism, reduction in serum cholesterol and immune system stimulation. Several studies in recent years have shown the benefits deriving from the ingestion of probiotics. The objective of this study was to screen ten potential probiotic organisms from total of 10 different food samples, milks products, and medicinal capsules were collected. The organisms identified on the basis of phenotypic characters and biochemical properties were Enterococci durans, Lactobacillus rhamnous, Lactobacillus plantarum, Lactobacillus paracasei, and Lactobacillus lactis. It was observed that similar types of probiotic organisms were isolated from food items, milk products and medicinal capsules.

  5. Ardrea characterisation of acidophilic micro-organisms isolated from gold mines in Marmato, Colombia

    Directory of Open Access Journals (Sweden)

    Edna Judith Márquez F.

    2008-02-01

    Full Text Available Mineral bio-oxidation improves the extraction of valuable metals and also decreases the impact caused by mining waste; however, the interactions between the micro-organisms so involved are little known. Double-layer solid culture media techniques and amplified ribosomal DNA restriction enzyme analysis (Ardrea, using Eco72I, Eco24I, XcmI and BsaAI enzymes, were used for characterising four micro-organisms isolated from gold mines located in Marmato, Colombia. This work was aimed at better understanding of native acidophilic micro-organisms’ microbial interactions in mixed cultures. Iron and sulphur oxidising isolates revealed similar restriction patterns to those previously reported for Acidithiobacillus ferrooxidans; however, one of them exhibited different colony morphology compared to previously reported morphology. The iron non-oxidising isolate presented a restriction pattern agreeing with theoretical analysis of Acidithiobacillus thiooxidans database sequences. ARDREA proved to be a viable technique for differentiating between At. ferrooxidans and At. thiooxidans; in turn, it enabled checking isolates’ identity with their physiological traits and colony morphology.

  6. Complex organic molecules along the accretion flow in isolated and externally irradiated protoplanetary disks

    CERN Document Server

    Walsh, Catherine; Nomura, Hideko; Millar, T J; Weaver, Susanna Widicus

    2014-01-01

    (Abridged) The birth environment of the Sun will have influenced the conditions in the pre-solar nebula, including the attainable chemical complexity, important for prebiotic chemistry. The formation and distribution of complex organic molecules (COMs) in a disk around a T Tauri star is investigated for two scenarios: (i) an isolated disk, and (ii) a disk irradiated externally by a nearby massive star. The chemistry is calculated along the accretion flow from the outer disk inwards using a comprehensive network. Two simulations are performed, one beginning with complex ices and one with simple ices only. For the isolated disk, COMs are transported without major alteration into the inner disk where they thermally desorb into the gas reaching an abundance representative of the initial assumed ice abundance. For simple ices, COMs efficiently form on grain surfaces under the conditions in the outer disk. Gas-phase COMs are released into the molecular layer via photodesorption. For the irradiated disk, complex ice...

  7. Membrane filtration of agro-industrial wastewaters and isolation of organic compounds with high added values.

    Science.gov (United States)

    Zagklis, Dimitris P; Paraskeva, Christakis A

    2014-01-01

    The aim of the current study was the exploitation of agro-industrial wastes or by-products such as olive mill wastewater (OMW) and defective wines. A cost-effective system for their maximum exploitation is suggested, using a combined process of membrane filtration and other physicochemical processes. Wastewaters are first treated in a membrane system (prefiltration, ultrafiltration, nanofiltration, and reverse osmosis) where pure water and other organic fractions (by-products) are obtained. Organic fractions, called hereafter byproducts and not wastes, are further treated for the separation of organic compounds and isolation of high added value products. Experiments were performed with OMW and defective wines as characteristic agro-industrial wastewaters. Profit from the exploitation of agro-industrial wastewaters can readily help the depreciation of the indeed high cost process of membrane filtration. The simple phenolic fraction of the OMW was successfully isolated from the rest of the waste, and problems occurring during winemaking, such as high volatile acidity and odours, were tackled. PMID:24434988

  8. Characterization of the Causal Organism of Soft Rot of Tomatoes and Other Vegetables and Evaluation of Its Most Aggressive Isolates

    OpenAIRE

    Asma Akbar; Musharaf Ahmad;   Azra;   Neelam; Sana Zeb Khan; Zahoor Ahmad

    2015-01-01

    To isolate the causal organism of soft rot of vegetables, diseased samples of potato, tomato, carrot, chilies, and bell pepper, were analyzed in the lab, using nutrient agar (NA) and/or the enrichment host (Bell pepper) technique. Successful isolations were purified by sub-culturing, identified as Erwinia carotovora subsp. carotovora through biochemical tests and their pathogenicity was confirmed through inoculation on green tomato fruits. The isolates were tested for their aggressiveness to ...

  9. A comparison of surface water natural organic matter in raw filtered water samples, XAD, and reverse osmosis isolates

    Science.gov (United States)

    Maurice, P.A.; Pullin, M.J.; Cabaniss, S.E.; Zhou, Q.; Namjesnik-Dejanovic, K.; Aiken, G.R.

    2002-01-01

    This research compared raw filtered waters (RFWs), XAD resin isolates (XAD-8 and XAD-4), and reverse osmosis (RO) isolates of several surface water samples from McDonalds Branch, a small freshwater fen in the New Jersey Pine Barrens (USA). RO and XAD-8 are two of the most common techniques used to isolate natural organic matter (NOM) for studies of composition and reactivity; therefore, it is important to understand how the isolates differ from bulk (unisolated) samples and from one another. Although, any comparison between the isolation methods needs to consider that XAD-8 is specifically designed to isolate the humic fraction, whereas RO concentrates a broad range of organic matter and is not specific to humics. The comparison included for all samples: weight average molecular weight (Mw), number average molecular weight (Mn), polydispersity (??), absorbance at 280nm normalized to moles C (??280) (RFW and isolates); and for isolates only: elemental analysis, % carbon distribution by 13C NMR, and aqueous FTIR spectra. As expected, RO isolation gave higher yield of NOM than XAD-8, but also higher ash content, especially Si and S. Mw decreased in the order: RO>XAD-8>RFW>XAD-4. The Mw differences of isolates compared with RFW may be due to selective isolation (fractionation), or possibly in the case of RO to condensation or coagulation during isolation. 13C NMR results were roughly similar for the two methods, but the XAD-8 isolate was slightly higher in 'aromatic' C and the RO isolate was slightly higher in heteroaliphatic and carbonyl C. Infrared spectra indicated a higher carboxyl content for the XAD-8 isolates and a higher ester:carboxyl ratio for the RO isolates. The spectroscopic data thus are consistent with selective isolation of more hydrophobic compounds by XAD-8, and also with potential ester hydrolysis during that process, although further study is needed to determine whether ester hydrolysis does indeed occur. Researchers choosing between XAD and RO

  10. Antipoliovirus Activity of the Organic Extract of Eupatorium buniifolium: Isolation of Euparin as an Active Compound

    OpenAIRE

    Muschietti, Liliana V.; Lucía V. Cavallaro; Martino, Virginia S.; María Florencia Visintini Jaime; Campos, Rodolfo H.

    2013-01-01

    The antiviral activity of the organic extract (OE) of Eupatorium buniifolium against poliovirus type 1 was determined by in vitro assays with an effective concentration 50 (EC50) of 23.3 ± 3.3 µg/mL. Bioassay-guided fractionation of the OE allowed the isolation of an active principle that was identified by spectroscopic methods (1H- and 13C-NMR, EI-MS, UV, and IR spectroscopy) as the benzofuran euparin. The plaque reduction assay in Vero cells was used to assess the antiviral activity of eupa...

  11. Purification and Characterization of Haloalkaline, Organic Solvent Stable Xylanase from Newly Isolated Halophilic Bacterium-OKH

    OpenAIRE

    Sanghvi, Gaurav; Jivrajani, Mehul; Patel, Nirav; Jivrajani, Heta; Bhaskara, Govinal Badiger; Patel, Shivani

    2014-01-01

    A novel, alkali-tolerant halophilic bacterium-OKH with an ability to produce extracellular halophilic, alkali-tolerant, organic solvent stable, and moderately thermostable xylanase was isolated from salt salterns of Mithapur region, Gujarat, India. Identification of the bacterium was done based upon biochemical tests and 16S rRNA sequence. Maximum xylanase production was achieved at pH 9.0 and 37°C temperature in the medium containing 15% NaCl and 1% (w/v) corn cobs. Sugarcane bagasse and whe...

  12. Two dimensional gel electrophoretic resolution of the polypeptides of mitochondria isolated from different rat organs

    International Nuclear Information System (INIS)

    A comparative study of rat liver, kidney, heart, spleen, and brain mitochondria were made by resolving their polypeptides by two dimensional gel electrophoresis and staining with silver. The mitochondria were purified using either a sucrose or a ficoll gradient and the purity monitored by enzyme markers. Mitochondria from each organ exhibited a characteristic 2-D gel pattern. There were many similarities and differences between the mitochondria isolated from different organs. Under optimal conditions, the liver mitochondria (40 μg) produced 200 to 250 spots. The total number of spots decreased in the order: liver > brain > heart, kidney > spleen. The liver mitochondria showed no spots in the pI 3H-pargyline, and the alpha- and beta-subunits of the F1 ATPase by comparing with published data

  13. Rapid isolation of gene homologs across taxa: Efficient identification and isolation of gene orthologs from non-model organism genomes, a technical report

    Directory of Open Access Journals (Sweden)

    Heffer Alison

    2011-03-01

    Full Text Available Abstract Background Tremendous progress has been made in the field of evo-devo through comparisons of related genes from diverse taxa. While the vast number of species in nature precludes a complete analysis of the molecular evolution of even one single gene family, this would not be necessary to understand fundamental mechanisms underlying gene evolution if experiments could be designed to systematically sample representative points along the path of established phylogenies to trace changes in regulatory and coding gene sequence. This isolation of homologous genes from phylogenetically diverse, representative species can be challenging, especially if the gene is under weak selective pressure and evolving rapidly. Results Here we present an approach - Rapid Isolation of Gene Homologs across Taxa (RIGHT - to efficiently isolate specific members of gene families. RIGHT is based upon modification and a combination of degenerate polymerase chain reaction (PCR and gene-specific amplified fragment length polymorphism (AFLP. It allows targeted isolation of specific gene family members from any organism, only requiring genomic DNA. We describe this approach and how we used it to isolate members of several different gene families from diverse arthropods spanning millions of years of evolution. Conclusions RIGHT facilitates systematic isolation of one gene from large gene families. It allows for efficient gene isolation without whole genome sequencing, RNA extraction, or culturing of non-model organisms. RIGHT will be a generally useful method for isolation of orthologs from both distant and closely related species, increasing sample size and facilitating the tracking of molecular evolution of gene families and regulatory networks across the tree of life.

  14. SIPCAn (Separation, Isolation, Purification, Characterization, and Analysis): A One-Term, Integrated Project for the Undergraduate Organic Laboratory

    Science.gov (United States)

    Dintzner, Matthew R.; Kinzie, Charles R.; Pulkrabek, Kimberly A.; Arena, Anthony F.

    2011-01-01

    SIPCAn, an acronym for separation, isolation, purification, characterization, and analysis, is presented as a one-term, integrated project for the first-term undergraduate organic laboratory course. Students are assigned two mixtures of unknown organic compounds--a mixture of two liquid compounds and a mixture of two solid compounds--at the…

  15. Isolation of a lytic bacteriophage against virulent Aeromonas hydrophila from an organized equine farm.

    Science.gov (United States)

    Anand, Taruna; Vaid, Rajesh Kumar; Bera, Bidhan Ch; Singh, Jitender; Barua, Sanjay; Virmani, Nitin; K, Rajukumar; Yadav, Neeraj Kumar; Nagar, Dinesh; Singh, Raj K; Tripathi, B N

    2016-04-01

    A bacteriophage (VTCCBPA6) against a pathogenic strain of Aeromonas hydrophila was isolated from the sewage of an organized equine breeding farm. On the basis of TEM analysis, phage belonged to family Myoviridae. PCR amplification and sequence analysis of gp23 gene (encoding for major capsid protein) revealed phylogenetic resemblance to T4 like virus genus. Protein profiling by SDS-PAGE also indicated its resemblance to T4 like phage group. However, the comparison of its gp23 gene sequence with previously reported phages showed similarity with T4-like phages infecting Enterobacteriaceae instead of Aeromonas spp. Thus, to our knowledge, this report points toward the fact that a novel/evolved phage might exist in equine environment against A. hydrophila, which can be potentially used as a biocontrol agent. PMID:26748732

  16. A 2D zinc-organic network being easily exfoliated into isolated sheets

    Science.gov (United States)

    Yu, Guihong; Li, Ruiqing; Leng, Zhihua; Gan, Shucai

    2016-08-01

    A metal-organic aggregate, namely {Zn2Cl2(BBC)}n (BBC = 4,4‧,4‧‧-(benzene-1,3,5-triyl-tris(benzene-4,1-diyl))tribenzoate) was obtained by solvothermal synthesis. Its structure is featured with the Zn2(COO)3 paddle-wheels with two chloride anions on axial positions and hexagonal pores in the layers. The exclusion of water in the precursor and the solvent plays a crucial role in the formation of target compound. This compound can be easily dissolved in alkaline solution and exfoliated into isolated sheets, which shows a novel way for the preparation of 2D materials.

  17. A STUDY OF EPIDEMIOLOGY AND ANTIMICROBIOLOGICAL PROFILE OF ORGANISM ISOLATED FROM NEONATAL SEPSIS PATIENTS

    Directory of Open Access Journals (Sweden)

    Ramesh

    2015-12-01

    Full Text Available INTRODUCTION Neonatal sepsis is one of the major cause of neonatal death In our country. It is a clinical syndrome characterized by signs and symptoms of infection with or without accompanying bacteraemia in first month of life it in compasses various systemic infections of new born such as septicaemia, meningitis, and urinary tract infections. Present study is a retrospective study in which all the date of neonatal sepsis which has positive blood culture admitted in the NISU Department of paediatrics Konaseema institute of medical science in last one and half year between June-2014 to Nov 2015 were collected. The most common organism isolated in our study was staphylococcus aureus that is 62.5%.

  18. Isolation of Hypervalent Group-16 Radicals and Their Application in Organic-Radical Batteries.

    Science.gov (United States)

    Imada, Yasuyuki; Nakano, Hideyuki; Furukawa, Ko; Kishi, Ryohei; Nakano, Masayoshi; Maruyama, Hitoshi; Nakamoto, Masaaki; Sekiguchi, Akira; Ogawa, Masahiro; Ohta, Toshiaki; Yamamoto, Yohsuke

    2016-01-20

    Using a newly prepared tridentate ligand, we isolated hypervalent sulfur and selenium radicals for the first time and characterized their structures. X-ray crystallography, electron spin resonance spectroscopy, and density functional theory calculations revealed a three-coordinate hypervalent structure. Utilizing the reversible redox reactions between hypervalent radicals and the corresponding anions bearing Li(+), we developed organic radical batteries with these compounds as cathode-active materials. Furthermore, an all-radical battery, with these compounds as the cathode and a silyl radical as the anode, was developed that exhibited a practical discharge potential of ∼ 1.8 V and stable cycle performance, demonstrating the potential of these materials for use in metal-free batteries that can replace conventional Li-ion batteries where Li is used in the metal form. PMID:26721786

  19. Quantifying and isolating stable soil organic carbon using long-term bare fallow experiment

    DEFF Research Database (Denmark)

    Barré, P; Eglin, T; Christensen, Bent Tolstrup;

    2010-01-01

    The stability of soil organic matter (SOM) is a major source of uncertainty in predicting atmospheric CO2 concentration during the 21st century. Isolating the stable soil carbon (C) from other, more labile, C fractions in soil is of prime importance for calibrating soil C simulation models......, and gaining insights into the mechanisms that lead to soil C stability. Long-term experiments with continuous bare fallow (vegetation-free) treatments in which the decay of soil C is monitored for decades after all inputs of C have stopped, provide a unique opportunity to assess the quantity of stable soil C....... We analyzed data from six bare fallow experiments of long-duration (>30 yrs), covering a range of soil types and climate conditions, and sited at Askov (Denmark), Grignon and Versailles (France), Kursk (Russia), Rothamsted (UK), and Ultuna (Sweden). A conceptual three pool model dividing soil C...

  20. Acid-base and copper-binding properties of three organic matter fractions isolated from a forest floor soil solution

    OpenAIRE

    van Schaik, Joris W. J.; Kleja, Dan B.; Gustafsson, Jon Petter

    2010-01-01

    Vast amounts of knowledge about the proton- and metal-binding properties of dissolved organic matter (DOM) in natural waters have been obtained in studies on isolated humic and fulvic (hydrophobic) acids. Although macromolecular hydrophilic acids normally make up about one-third of DOM, their proton- and metal-binding properties are poorly known. Here, we investigated the acid-base and Cu-binding properties of the hydrophobic (fulvic) acid fraction and two hydrophilic fractions isolated from ...

  1. Large nonlinear optical activity from hybrid inorganic–organic films with fluorinated benzene as isolation group

    International Nuclear Information System (INIS)

    Two azo chromophores containing fluorinated benzene and alkyl chain as isolation group were designed and synthesized, respectively, and the corresponding alkoxysilane dyes were obtained by coupling 3-isocyanatopropyltriethoxysilane with the chromophores. The molecular structures were verified by elemental analysis, hydrogen nuclear magnetic resonance, and Fourier transform infrared spectrum. Followed by a sol–gel process of the alkoxysilane dyes, inorganic–organic hybrid films were prepared by spin-coating. After electric poling, these hybrid films show the higher nonlinear optical (NLO) response than their analog containing chromophore DR1. Furthermore, the fluorinated benzene group exhibits better enhanced effect than the flexible alkyl group. The highest NLO coefficients (d33) of the hybrid film containing fluorinated benzene group was determined to be 140.5 pm V−1 at the chromophore concentration of 40%. - Highlights: • Inorganic-organic hybrid films are prepared via sol-gel process of alkoxysilane dyes • Nonlinear optical properties of hybrid films are investigated • Fluorinated benzene group effectively improves the nonlinear optical property

  2. Isolation of Renewable Phenolics by Adsorption on Ultrastable Hydrophobic MIL-140 Metal-Organic Frameworks.

    Science.gov (United States)

    Van de Voorde, Ben; Damasceno Borges, Daiane; Vermoortele, Frederik; Wouters, Robin; Bozbiyik, Belgin; Denayer, Joeri; Taulelle, Francis; Martineau, Charlotte; Serre, Christian; Maurin, Guillaume; De Vos, Dirk

    2015-09-21

    The isolation and separation of phenolic compounds from aqueous backgrounds is challenging and will gain in importance as we become more dependent on phenolics from lignocellulose-derived bio-oil to meet our needs for aromatic compounds. Herein, we show that highly stable and hydrophobic Zr metal-organic frameworks of the MIL-140 type are effective adsorbent materials for the separation of different phenolics and far outperform other classes of porous solids (silica, zeolites, carbons). The mechanism of the hydroquinone-catechol separation on MIL-140C was studied in detail by combining experimental results with computational techniques. Although the differences in adsorption enthalpy between catechol and hydroquinone are negligible, the selective uptake of catechol in MIL-140C is explained by its dense π-π stacking in the pores. The interplay of enthalpic and entropic effects allowed separation of a complex, five-compound phenol mixture through breakthrough over a MIL-140C column. Unlike many other metal-organic frameworks, MIL-140C is remarkably stable and maintained structure, porosity and performance after five adsorption-desorption cycles. PMID:26373364

  3. Low levels of persistent organic pollutants (POPs) in New Zealand eels reflect isolation from atmospheric sources

    International Nuclear Information System (INIS)

    Polychlorinated biphenyls (PCBs) and organic pesticides (i.e., DDTs) were measured in long finned eels (Anguilla dieffenbachii) in 17 streams on the west coast of South Island, New Zealand. Very low levels of PCBs and low levels of ppDDE were found. The concentrations of PCBs and ppDDE were not correlated within sites indicating that different processes determined the levels of the two pollutants in New Zealand eels. The PCBs probably originate from atmospheric transport, ppDDE levels are determined by land use and are higher in agriculture areas. The low contamination level of these aquatic systems seems to be a function of a low input from both long and short-range transport as well as few local point sources. No correlation could be found between lipid content and persistent organic pollutants (POPs) concentration (as shown in previous studies) in the eels which could be explained by low and irregular intake of the pollutants. - Low levels of PCBs found in New Zealand eels reflect isolation from atmospheric sources while DDTs levels are determined by land use

  4. Influence of Zinc Supplementation in Acute Diarrhea Differs by the Isolated Organism

    Directory of Open Access Journals (Sweden)

    Archana B. Patel

    2010-01-01

    Full Text Available Zinc supplementation is recommended in all acute diarrheas in children from developing countries. We aimed to assess whether zinc supplementation would be equally effective against all the common organisms associated with acute diarrheas. We used data on 801 children with acute diarrhea recruited in a randomized, double blind controlled trial (ISRCTN85071383 of zinc and copper supplementation. Using prespecified subgroup analyses, multidimensionality reduction analyses, tests of heterogeneity, and stepwise logistic regression for tests of interactions, we found that the influence of zinc on the risk of diarrhea for more than 3 days depended on the isolated organism—beneficial in Klebsiella, neutral in Esherichia coli and parasitic infections, and detrimental in rotavirus coinfections. Although we found similar results for the outcome of high stool volume, the results did not reach statistical significance. Our findings suggest that the current strategy of zinc supplementation in all cases of acute diarrheas in children may need appropriate fine tuning to optimize the therapeutic benefit based on the causative organism, but further studies need to confirm and extend our findings.

  5. Whole-organ isolation approach as a basis for tissue-specific analyses in Schistosoma mansoni.

    Directory of Open Access Journals (Sweden)

    Steffen Hahnel

    Full Text Available BACKGROUND: Schistosomiasis is one of the most important parasitic diseases worldwide, second only to malaria. Schistosomes exhibit an exceptional reproductive biology since the sexual maturation of the female, which includes the differentiation of the reproductive organs, is controlled by pairing. Pathogenicity originates from eggs, which cause severe inflammation in their hosts. Elucidation of processes contributing to female maturation is not only of interest to basic science but also considering novel concepts combating schistosomiasis. METHODOLOGY/PRINCIPAL FINDINGS: To get direct access to the reproductive organs, we established a novel protocol using a combined detergent/protease-treatment removing the tegument and the musculature of adult Schistosoma mansoni. All steps were monitored by scanning electron microscopy (SEM and bright-field microscopy (BF. We focused on the gonads of adult schistosomes and demonstrated that isolated and purified testes and ovaries can be used for morphological and structural studies as well as sources for RNA and protein of sufficient amounts for subsequent analyses such as RT-PCR and immunoblotting. To this end, first exemplary evidence was obtained for tissue-specific transcription within the gonads (axonemal dynein intermediate chain gene SmAxDynIC; aquaporin gene SmAQP as well as for post-transcriptional regulation (SmAQP. CONCLUSIONS/SIGNIFICANCE: The presented method provides a new way of getting access to tissue-specific material of S. mansoni. With regard to many still unanswered questions of schistosome biology, such as elucidating the molecular processes involved in schistosome reproduction, this protocol provides opportunities for, e.g., sub-transcriptomics and sub-proteomics at the organ level. This will promote the characterisation of gene-expression profiles, or more specifically to complete knowledge of signalling pathways contributing to differentiation processes, so discovering involved

  6. Induction of arthritis with Mycoplasma hyosynoviae in pigs: Clinical response and re-isolation of the organism from body fluids and organs

    DEFF Research Database (Denmark)

    Hagedorn-Olsen, T.; Nielsen, N.C.; Friis, N.F.

    1999-01-01

    systemic phase. An apparent persistent infection of the tonsils became established in all the pigs. M. hyosynoviae spreads via the blood to different organs from which it could be re-isolated during the acute phase of the infection. In general, M. hyosynoviae was recovered from joints from day 3 until day...

  7. Use of radiocarbon and spectroscopic analyses to characterise soil organic matter pools isolated using different fractionation techniques.

    Science.gov (United States)

    Miller, Gemma; Cloy, Joanna; Garnett, Mark; Sohi, Saran; Rees, Robert; Griffiths, Bryan

    2015-04-01

    Experimental division of soil organic matter (SOM) into functional pools has the potential to improve soil C modelling. Soil physical fractionation techniques seek to quantify these pools, however the fractions isolated vary in number, size, ecological role and composition. The use of different techniques to quantify soil C fractions in different studies presents a question - do similar fractions isolated by different methods fit the same conceptual definition? This study examined a sandy loam from the south-west of Scotland, sampled in summer, which had been under grassland management for at least 20 years. We compared average 14C ages of SOM fractions isolated using three published and frequently applied physical fractionation methods (1) a density separation technique isolating three fractions - free light (FLF) 1.8 g cm 3 (Sohi et al, 2001); (2) a combined physical and chemical separation isolating five fractions: sand and aggregates (S+A) > 63 µm and > 1.8 g cm-3, particulate organic matter (POM) > 63 µm and 45 µm, residual organic carbon (rSOC) the residue left after s+c is oxidised with NaOCl, and dissolved organic carbon (DOC) 4000 years BP for DOC and POM. Both Method 3 fractions were dominated by modern C. The average 14C ages of FLF, IALF, DOC and POM were surprisingly higher than the mineral bound fractions, although they made up a relatively small proportion of the total organic C (8.4 and 12.4 % for Methods 1 and 2 respectively). These results will be discussed alongside data from FTIR and UV-vis spectroscopy. The characterisation of physically separated organic matter pools is likely to provide improved opportunities for modelling the long term behaviour of SOM on the basis of defined chemical and physical properties. References Sohi, S.P., Mahieu, N., Arah, J.R.M., Powlson, D.S., Madari, B. and Gaunt J.L. (2001) A procedure for isolating soil organic matter fractions suitable for modelling. Soil Science Society of America Journal 65

  8. Site-specific distribution and competitive ability of indigenous bean-nodulating rhizobia isolated from organic fields in Minnesota.

    Science.gov (United States)

    Wongphatcharachai, Manoosak; Wang, Ping; Staley, Christopher; Chun, Chan Lan; Ferguson, John A; Moncada, Kristine M; Sheaffer, Craig C; Sadowsky, Michael J

    2015-11-20

    Organic dry bean production systems have received increasing interest in many regions of the US, including Minnesota. Thus, improving biological N2 fixation would be highly beneficial for organic crop production. To date, only limited work has been done to select efficient N2-fixing rhizobia for organic dry bean production. In this study, soil samples from 25 organic fields in Minnesota, with a previous cropping history of dry beans, soybeans or both, were collected during May to July 2012. Genetic diversity of indigenous dry bean-rhizobia (511 isolates) was determined by using horizontal, fluorophore-enhanced, repetitive, extragenic, and palindromic-PCR (HFERP) DNA fingerprinting and isolates were classified as belonging to 58 different genotypes. The more abundant rhizobia isolated from bean nodules comprised 35.6% of the population. None of the isolates were identical to commonly-used commercial strains used in the U.S., including Rhizobium tropici CIAT899. Seventeen predominant genotypes were shown to represent two main species, Rhizobium leguminosarum bv. phaseoli (67.1%) and Rhizobium etli (30.2%). One of the indigenous strains, orgK9, displayed efficient N2-fixation and competitive ability relative to the commercial strains tested. The lack of large numbers of indigenous dry bean-rhizobia at most study sites will be useful to avoid competition problems between inoculant strains and indigenous rhizobia. This will allow inoculation with highly effective N2-fixing rhizobia, thus resulting in improved crop productivity. Our results highlight the existence of site-specific rhizobial genotypes in different organic fields and identify strains that may prove useful as novel inoculants for organic dry bean production systems. PMID:26403588

  9. Quantifying and isolating stable soil organic carbon using long-term bare fallow experiments

    Directory of Open Access Journals (Sweden)

    P. Barré

    2010-11-01

    Full Text Available The stability of soil organic matter (SOM is a major source of uncertainty in predicting atmospheric CO2 concentration during the 21st century. Isolating the stable soil carbon (C from other, more labile, C fractions in soil is of prime importance for calibrating soil C simulation models, and gaining insights into the mechanisms that lead to soil C stability. Long-term experiments with continuous bare fallow (vegetation-free treatments in which the decay of soil C is monitored for decades after all inputs of C have stopped, provide a unique opportunity to assess the quantity of stable soil C. We analyzed data from six bare fallow experiments of long-duration (>30 yrs, covering a range of soil types and climate conditions, and sited at Askov (Denmark, Grignon and Versailles (France, Kursk (Russia, Rothamsted (UK, and Ultuna (Sweden. A conceptual three pool model dividing soil C into a labile pool (turnover time of a several years, an intermediate pool (turnover time of a several decades and a stable pool (turnover time of a several centuries or more fits well with the long term C decline observed in the bare fallow soils. The estimate of stable C ranged from 2.7 g C kg−1 at Rothamsted to 6.8 g C kg−1 at Grignon. The uncertainty associated with estimates of the stable pool was large due to the short duration of the fallow treatments relative to the turnover time of stable soil C. At Versailles, where there is least uncertainty associated with the determination of a stable pool, the soil contains predominantly stable C after 80 years of continuous bare fallow. Such a site represents a unique research platform for characterization of the nature of stable SOM and its vulnerability to global change.

  10. ISOLATING AND FRACTIONATING ORGANIC TOXICANTS IN SEDIMENTS: EVALUATION OF AN EXPERIMENTAL APPROACH

    Science.gov (United States)

    Most solid-phase sediment TIE techniques for organic chemicals have been focused on solid phase sorptive techniques, such as amending contaminated sediments with the carbonaceousresin, Ambersorb coconut charcoal, or XAD resin to reduce toxicity caused by organic contaminants. Cha...

  11. Isolation and Identification of The Causative Organisms of Conjunctivitis in Healthy Subject and Patients

    International Nuclear Information System (INIS)

    An investigation concerning the isolation of bacterial flora from healthy and patient eyes was carried out. Micrococcus lylae and Moraxella lacunata represent the normal strains while Acinetobacter baumanni, Klebsiella pneumoniae, Moraxella cuniculi, M. ovis, Neisseria lactamica and Streptococcus pyogenes were isolated from infected eyes only. NaCl of concentration 7.5% (w/v) can inhibit the growth of all isolated strains. The action of UV on population of bacteria was varied according to the species. Pseudomonas aeruginosa was resistant to rifampcin, erythromycin, fucidin and bacitracin. Some bacterial strains have a capsule containing carbohydrate and many have carbohydrate and amino acids containing capsules. The detected predominant chemical subunits in capsule structure were glucose in Moraxella ovis, and fructose and mannose in M. cuniculi.

  12. Enterobacteriaceae and related organisms isolated from shell eggs collected during commercial processing.

    Science.gov (United States)

    Musgrove, M T; Northcutt, J K; Jones, D R; Cox, N A; Harrison, M A

    2008-06-01

    In the United States, commercial shell eggs are washed and graded before retail. Since passage of the Egg Products Inspection Act in 1971, processing guidelines have been set to ensure that external and internal characteristics are maintained. However, less is known about how commercial processing affects the safety of shell eggs. To identify enteric bacteria entering plants and persisting throughout processing, eggs were collected from 3 US commercial shell egg-processing plants on 3 separate visits. On each plant visit, 12 eggs were collected from each of 12 sites along the processing line: accumulator, prewash rinse, first washer, second washer, sanitizer rinse, dryer, oiler, check detection/scales, 2 egg grader/packer head lanes, rewash belt entrance, and rewash belt exit. Each egg was sampled by a rinse technique, and the rinsate was plated onto violet red bile glucose agar with overlay for the detection and enumeration of Enterobacteriaceae. From each plate, up to 5 colonies were randomly selected and isolated for identification to genus or species by using biochemical tests. Several genera and species were detected at each of the 3 plants. Sites from which the greatest numbers of isolates were identified were those collected from eggs during preprocessing (accumulator, prewash rinse) or from eggs judged as dirty (rewash belt entrance or exit). Sites yielding the smallest number of isolates were those during or at the end of processing. Escherichia coli and Enterobacter spp. were isolated from each of the 9 plant visits. Other genera isolated from at least 1 of the 3 plants included Cedecea, Citrobacter, Erwinia, Hafnia, Klebsiella, Kluyvera, Leclercia, Morganella, Proteus, Providencia, Rahnella, Salmonella, and Serratia. Non-Enterobacteriaceae isolated and identified included Aeromonas, Chryseomonas, Listonella, Pseudomonas, Sphingobacterium, Vibrio, and Xanthomonas. All of the genera and species were recovered less frequently from fully processed eggs than

  13. Characterization of clay minerals and organic matter in shales: Application to high-level nuclear waste isolation

    International Nuclear Information System (INIS)

    The objective of the Sedimentary Rock Program at the Oak Ridge National Laboratory is to conduct investigations to assess the potential for shale to serve as a host medium for the isolation of high-level nuclear wastes. The emphasis on shale is a result of screening major sedimentary rock types (shale, sandstone, carbonate , anhydrite, and chalk) for a variety of attributes that affect the performance of repositories. The retardation of radionuclides was recognized as one of the potentially favorable features of shale. Because shale contains both clay minerals and organic matter, phases that may provide significant sorption of radioelement, the characterization of these phases is essential. In addition, the organic matter in shale has been identified as a critical area for study because of its potential to play either a favorable (reductant) or deleterious (organic ligands) role in the performance of a repository sited in shale. 36 refs., 36 figs., 10 tabs

  14. Evaluation of supercritical fluid extraction/gas chromatography/matrix isolation-infrared spectrometry for analysis of organic compounds

    International Nuclear Information System (INIS)

    Analysis of soil samples for organic compounds typically first requires Soxhlet extraction or sonication. These processes are time consuming and generate large amounts of waste solvent. Supercritical fluid extraction (SFE), which uses a supercritical fluid such as carbon dioxide, has recently been shown to extract organic compounds from soil samples in good yields. Moreover, SFE does not generate waste solvent and can be performed rapidly. Gas Chromatography/Matrix Isolation-Infrared Spectrometry (GC/MI-IR) has been used in our laboratories for determining organic compounds present in extracts from various matrices. The authors have interfaced an SFE extraction apparatus to GC/MI-IR instruments. In this paper the utility of SPE/GC/MI-IR instrumentation is discussed

  15. Genotypic diversity of Lactobacillus sanfranciscensis strains isolated from French organic sourdoughs.

    Science.gov (United States)

    Lhomme, Emilie; Onno, Bernard; Chuat, Victoria; Durand, Karine; Orain, Servane; Valence, Florence; Dousset, Xavier; Jacques, Marie-Agnès

    2016-06-01

    Lactobacillus sanfranciscensis is the predominant key lactic acid bacterium in traditionally fermented sourdoughs. Despite its prevalence, sourdough and their related breads could be different regarding their physicochemical and sensorial characteristics. The intraspecific diversity of L. sanfranciscensis might explain these observations. Fifty-nine strains isolated from French sourdoughs were typed by a polyphasic approach including Multilocus Sequence Typing (MLST) and Pulsed-field Gel Electrophoresis (PFGE), in order to study their genotypic diversity. MLST scheme can be reduced from six to four gene fragments (gdh, gyrA, nox and pta) without a major loss of discrimination between strains. The genes mapA and pgmA are not good candidates for inclusion in an MLST scheme to type L. sanfranciscensis strains, as they could not be amplified for a set of 18 strains among the 59 studied. This method revealed 20 sequence types (STs). Of these, 19 STs were grouped in one clonal complex, showing a strong relatedness between these strains. PFGE using SmaI discriminated 41 pulsotypes and so distinguished isolates better than the MLST scheme. Both genotypic methods indicate a low diversity between strains isolated from the same sourdough and a higher diversity between strains isolated from different sourdoughs, suggesting an influence of baker practices and/or environmental conditions on the selection of strains. The use of these two methods targeting genetic variations gives an optimal genotypic characterization of L.sanfranciscensis strains. PMID:27015297

  16. Broad-Spectrum Antimicrobial Epiphytic and Endophytic Fungi from Marine Organisms: Isolation, Bioassay and Taxonomy

    Directory of Open Access Journals (Sweden)

    Yan-Hua Zhu

    2009-04-01

    Full Text Available In the search for new marine derived antibiotics, 43 epi- and endophytic fungal strains were isolated from the surface or the inner tissue of different marine plants and invertebrates. Through preliminary and secondary screening, 10 of them were found to be able to produce broad-spectrum antimicrobial metabolites. By morphological and molecular biological methods, three active strains were characterized to be Penicillium glabrum, Fusarium oxysporum, and Alternaria alternata.

  17. Complete Nucleotide Sequences and Genome Organization of Two Pepper Mild Mottle Virus Isolates from Capsicum annuum in South Korea.

    Science.gov (United States)

    Choi, Seung-Kook; Choi, Gug-Seoun; Kwon, Sun-Jung; Yoon, Ju-Yeon

    2016-01-01

    The complete genome sequences of pepper mild mottle virus (PMMoV)-P2 and -P3 were determined by the Sanger sequencing method. Although PMMoV-P2 and PMMoV-P3 have different pathogenicity in some pepper cultivars, the complete genome sequences of PMMoV-P2 and -P3 are composed of 6,356 nucleotides (nt). In this study, we report the complete genome sequences and genome organization of PMMoV-P2 and -P3 isolates from pepper species in South Korea. PMID:27198033

  18. ISOLATING AND EVALUATING ORGANIC TOXICANTS IN SEDIMENTS: EVALUATION OF AN EXPERIMENTAL APPROACH

    Science.gov (United States)

    Most solid-phase sediment toxicity identification and evaluation (TIE) techniques for organic chemicals have been focused on solid phase sorptive techniques, such as amending contaminated sediments with the carbonaceous resin, Ambersorb, coconut charcoal, or XAD resin to reduce t...

  19. Isolation, identification and characterization of organic solvent tolerant protease from Bacillus sp. DAF-01

    OpenAIRE

    Arastoo Badoei-Dalfard; Mostafa Amiri-Bahrami; Ali Riahi-Madvar; Zahra Karami; Mohammad Ali Ebrahimi

    2012-01-01

    Introduction: Organic solvent-tolerant bacteria are relatively novel extermophilic microorganisms, which can produce organic tolerant protease with capacity of being used in industrial biotechnology for producing high-value compounds. Therefore, finding of these bacteria has drawn much researchers attention nowadays. Materials and Methods: In this project, samples were collected from a hot spring, located in Jiroft. Samples were incubated in medium supplemented with cyclohexane and toluene fo...

  20. Isolation of Organic and Usakti Deep Well Water Turbidity Using Dual Media Direct Filtration

    International Nuclear Information System (INIS)

    The study of direct filtration deep well water with permanganate organic content between using 15.3 -16.2 mg/l and turbidity after additional 210 mg/l alum, 12 - 14 Ntu, has been carried out. The type of media were used dual media consist of anthracite 60 cm height and silica sand 30 cm height. The variety of three media grains types have shown effectiveness not same. The effectiveness order of filtration on initial filtration were effective diameter of anthracite (d10a) 0.90 mm and effective diameter of silica sand (d10s) 0.38 mm > d10a 0.90 mm and d10s 0.55 mm > d10a 1.57 mm and d10s 0.55 mm; but then the effectiveness change after efluen turbidity greater than 0.38 turbidity of influent to be, d10a 0.90 mm and d10s 0.55 mm > d10a 1.57 mm and d10s 0.55 mm >, d10a 0.90 mm and d10s 0.38 mm. The breakthrough curves have been illustrated as correlation between efluen turbidity to water production per surface area per porosity of filter have two filtration constants, K and two proportional constants, λ0 respectively where before these constants had respectively one value in conventional single media filtrations. The equation of permanganate-organic efluen in filtration, Cet as water production, Π per surface area of filter, A per porosity, ε, and determination of organic based on specifically suspension organic content and specifically non soluble organic fraction, f, have shown the significant results with determinant coefficients, r2 = 0.8524 - 0.9216. The equation form was Cet = f. Co + Co (1 - f)/[1 + exp{-K.Π/A.ε + In (eλ0L -1)}]. C0 is influent organic total. (author)

  1. Impact of Isolated Burns on Major Organs: A Large Animal Model Characterized.

    Science.gov (United States)

    Burmeister, David M; McIntyre, Matthew K; Baker, Bryan A; Rizzo, Julie A; Brown, Ammon; Natesan, Shanmugasundaram; Chung, Kevin K; Christy, Robert J

    2016-09-01

    Severe burn results in systemic inflammatory response syndrome (SIRS) and multiple organ dysfunction (MOD). Currently, large-animal models of burn-induced SIRS/MOD mostly use secondary insults resulting in a paucity of knowledge on the effect of burn alone on different organ systems. The objective of the current study was to develop and characterize a large animal model of burn-induced SIRS over the course of 2 weeks. Yorkshire swine (n = 16) were randomized to sham controls (n = 4) or 40% total body surface area contact burns (n = 6 at 2 and 14 days post-burn). Blood chemistry and complete blood count analyses were performed at baseline and post-burn days 1, 2, 3, 7, 10, and 14. Upon euthanasia, tissue samples were taken for histopathology. Burns were found to be full thickness and did not re-epithelialize. SIRS was evidenced by increased body temperature, respiration rate, pulse, and white blood cell count for the duration of the experiment. Both acute liver injury and acute kidney injury were induced as determined biochemically and histologically. Histology also revealed atelectasis of the lungs which was associated with increased myeloperoxidase activity. Intestinal structure as well as enterocyte homeostasis was also disrupted. All of these organ abnormalities recovered to varying degrees by 14 days post-burn. We report a unique reproducible large animal model of burn-induced SIRS that can be tailored to specific organ systems for investigation into potential immunomodulatory interventions that prevent organ failure or promote organ recovery after burn injury. PMID:27380531

  2. Organic extracts with antimicrobial activity from Penicillium sp (Moniliales) isolated from the sponge ircinia felix (Porifera : Demospongiae)

    International Nuclear Information System (INIS)

    This research expresses the potential of the bacterial activity present in the organic extracts obtained from Penicillium sp., isolated from the e sponge Incr. felix. This activity was evaluated through agar diffusion test and Minimal Inhibitory Concentration (MIC). The susceptibility trials of organic fractions were carried out against Staphylococcus aureus, S. epidermidis. Bacillus cereus and B. subtilis. The use of the chromatographic techniques (CLV and TLC), permitted to obtain bioactive organic extracts of different polarities, of which only the EtOAc and MeOH fractions inhibited the growth of the bacteria used. Of the EtOAc fractionation, only fraction number 3 EtOAc/Hex presented greatest activity against the Gram-positive bacteria. Number 1 EtOAc/Hex fraction increased its activity against S. aureus (24 mm) and S. epidermidis (25 mm), which can be explained by the loss of possible antagonistic effect during the fractionation process. The CMI trials were carried out for the EtOAc number I subtraction against S. aureus, S. epidermidis, B. cereus and B. subtilis, wich was clinical interest, and shows the potential of this organic extract as antimicrobial agent. (Author)

  3. CONSTANTS FOR MERCURY BINDING BY DISSOLVED ORGANIC MATTER ISOLATES FROM THE FLORIDA EVERGLADES. (R827653)

    Science.gov (United States)

    Dissolved organic matter (DOM) has been implicated as an important complexing agent for Hg that can affect its mobility and bioavailability in aquatic ecosystems. However, binding constants for natural Hg-DOM complexes are not well known. We employed a competitive ligand appro...

  4. Examinations Of The Matrix Isolation Fourier Transform Infrared Spectra Of Organic Compounds.

    Science.gov (United States)

    Coleman, W. M.

    1989-12-01

    Matrix isolation Fourier transform infrared spectra (MI/FT-IR), mass spectra (MS), carbon-13 Nuclear Magnetic Resonance (13C-NMR) spectra, condensed phase infrared spectra and vapor phase infrared (IR) spectra are presented for a series of terpene compounds. Subtle differences in positional and configurational isomers commonly found with terpenes could be easily detected by the. MI/FT-IR spqctra. The results are comparable in some aspects to those obtainable from IJC-NMR and thin film IR, however, most importantly, they are acquired at the low nanogram level for MI/FTIR as compared to the milligram level for the other techniques. These results represent an advance in the technology available for the analysis of complex mixtures such as essential oils containing terpene-like molecules.

  5. Enterobacteriaceae and related organisms isolated from nest run cart shelves in commercial shell egg processing facilities.

    Science.gov (United States)

    Musgrove, M T; Jones, D R; Shaw, J D; Sheppard, M; Harrison, M A

    2009-10-01

    Enterobacteriaceae, including Salmonella, may be recovered from foods and processing facilities. High levels of Enterobacteriaceae in the processing plant environment can be an indication of inadequate sanitation. This experiment was designed to determine if nest run egg carts serve as reservoirs for Enterobacteriaceae. Eggs that are produced by hens not housed in buildings connected to the processing plant are referred to as nest run. Many of these eggs are transported to the plant on carts to be processed. Two plants in the southeastern United States were sampled. On each of 3 visits, 5 shelves on each of 5 carts were sampled (n=25/visit). A 12x12 cm area on each shelf was swabbed with a sterile gauze pad moistened with PBS and transported on ice back to the laboratory. Enterobacteriaceae were enumerated using violet red bile glucose agar incubated at 37 degrees C for 24 h. There was 100% prevalence for Enterobacteriaceae at plant A with an average 3.8 log10 cfu/mL swab diluent. Plant B had 90% prevalence for Enterobacteriaceae with an average 3.2 log10 cfu/mL swab diluent. Two randomly selected isolates from each positive sample were recultured 3 times to increase the likelihood of clonality and were then identified biochemically. Of the 124 isolates analyzed, genera identified were Citrobacter spp., Escherichia spp., Enterobacter spp., Klebsiella spp., Hafnia spp., Kluyvera spp., Leclercia spp., and Salmonella spp. Pseudomonas spp. was the only non-Enterobacteriaceae identified by our methods. This work demonstrates that nest run egg carts serve as reservoirs for Enterobacteriaceae in the shell egg processing environment. PMID:19762864

  6. Bacterial Agents Andantibiogram of Most Common Isolated Organisms from Hands of Surgical Team Members after Scrubbing

    Directory of Open Access Journals (Sweden)

    PS Mohseni- Meybodi

    2008-04-01

    Full Text Available Introduction: Many post-surgical wound infections in hospitals cause morbidity and morality of patients and these are usually transmitted via hands of surgical personnel. The aim of the present study was to detect and antibiogram the bacterial agents following scrubbing of hands of surgical personnel before operation. Methods: Hands of 134 personnels of operation room were swabbed following scrubbing with antiseptic Betadine solution. Swab samples were inoculated on selective and differential media such as blood ager, McConky and manitol salt agar(MSA. Following incubation of media at 37c° for 24hr, bacterial species were identified using differential related tests. The isolated species were than antibiogramed and the results together with other data was analysed by SPSS software program. Results: Of the total of 134 cases, 81(60.4% were male and 53(39.6% female. The mean scrub time for each person was (206.1+/-103.2 seconds; 6 to 60 seconds base change. Increasing time of scrub was significantly correlated with decreasing rate of bacteria (P=0.003, (R=-0.254. Contamination was present in 129(96.3% cases following scrubbing. Maximum contamination was observed in nails (92.5%. Average number of bacteria for each individual was between 0 and 159. 62.6% of isolated bacteria were non- staphylococci and 7.7% were S. aureus. Vancomycin and ceftizoxim were the most sensitive, while penicillin was the least sensitive antibiotic. Conclusion: Results revealed that hand contamination was more than the expected standard level. Therefore, regarding the critical task of surgical personnel, training of all operation room staff is highly recommended to minimize the rate of contamination.

  7. Drug resistance of organisms isolated from feces of laboratory mice and rats.

    Science.gov (United States)

    Maejima, K; Urano, T; Tamura, H; Terakado, N

    1980-01-01

    A total of 248 strains of EScherichia coli, 132 of Staphylococcus epidermidis, 137 of Streptococcus faecalis and 89 of STr. faecium were collected from feces of 40 mice and 36 rats of 8 colonies in 1978, and drug resistance were examined by an agar dieution method using 23 antibiotics. The results indicated a positive relation between use of antibiotics and appearance of multiple drug resistant organisms. PMID:6772455

  8. ISOLATION AND CHARACTERIZATION OF BACTERIA FOR REMOVAL OF SULFUR IN ORGANIC COMPOUNDS

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    1. IntroductionThe presence of sulfur in fossil fuels contributesto corrosion of production and refining equipments,when burning these high-sulfur fuels, it will releasesulfur oxides into the atmosphere, which leading tothe formation of acid rain.Various processes have been developed for theremoval of sulfur compounds from fossil fuel.Inorganic sulfur can be reduced through physicalbeneficiation techniques, but organically boundsulfur is difficult to be removed by these ways 1142].Biological desulfurization...

  9. Triplet photochemistry of effluent and natural organic matter in whole water and isolates from effluent-receiving rivers.

    Science.gov (United States)

    Bodhipaksha, Laleen C; Sharpless, Charles M; Chin, Yu-Ping; Sander, Michael; Langston, William K; MacKay, Allison A

    2015-03-17

    Effluent organic matter (EfOM), contained in treated municipal wastewater, differs in composition from naturally occurring dissolved organic matter (DOM). The presence of EfOM may thus alter the photochemical production of reactive intermediates in rivers that receive measurable contributions of treated municipal wastewater. Quantum yield coefficients for excited triplet-state OM (3OM*) and apparent quantum yields for singlet oxygen (1O2) were measured for both whole water samples and OM isolated by solid phase extraction from whole water samples collected upstream and downstream of municipal wastewater treatment plant discharges in three rivers receiving differing effluent contributions: Hockanum R., CT (22% (v/v) effluent flow), E. Fork Little Miami R., OH (11%), and Pomperaug R., CT (6%). While only small differences in production of these reactive intermediates were observed between upstream and downstream whole water samples collected from the same river, yields of 3OM* and 1O2 varied by 30-50% between the rivers. Apparent quantum yields of 1O2 followed similar trends to those of 3OM*, consistent with 3OM* as a precursor to 1O2 formation. Higher 3OM* reactivity was observed for whole water samples than for OM isolates of the same water, suggesting differential recoveries of photoreactive moieties by solid phase extraction. 3OM* and 1O2 yields increased with increasing E2/E3 ratio (A254 nm divided by A365 nm) and decreased with increasing electron donating capacities of the samples, thus exhibiting trends also observed for reference humic and fulvic acid isolates. Mixing experiments with EfOM and DOM isolates showed evidence of quenching of triplet DOM by EfOM when measured yields were compared to theoretical yields. Together, the results suggest that effluent contributions of up to 25% (v/v) to river systems have a negligible influence on photochemical production of 3OM* and 1O2 apparently because of quenching of triplet DOM by EfOM. Furthermore, the results

  10. [Antimicrobial activity of organic extracts isolated from Aplysina fistularis (Demospongiae: Aplysinidae)].

    Science.gov (United States)

    Morales, T; Cubero, J; Lanz, Z; Gómez-Guiñán, Y; Segnini-Bravo, M I

    2000-12-01

    Organic extracts of the sponge Aplysina fistularis (Pallas 1766) were tested for antimicrobial activity against Gram positive bacteria (Staphylococcus aureus) and Gram negative bacteria (Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa). The minimal inhibitory concentration (MIC) and toxic activity of extract were determined. Susceptibility trials of organic fractions obtained by VLC: Hexane, EtOAc and CHCl3 showed that EtOAc fraction has antibacterial activity against E. coli, while CHCl3 fraction inhibited E. coli and S. aureus growth. The later refractioning of EtOAc fraction and the biodirected assays showed that fractions F12 and F13 of EtOAc/Hex and EtOAc F14 were bioactive against Gram positive and Gram negative bacteria. Only EtOAc/MeOH Sf2 from subfractionig of EtOAc F14 produced inhibition for E. coli and S. aureus. In Sf2 EtOAc/MeOH, MIC was moderate for S. aureus (MIC > 256 g/ml). F4 CHCl3/MeOH produced a high inhibition in S. aureus (MIC = 0.125 g/ml) and for E. coli (MIC > 16 g/ml). F10 CHCl3/MeOH showed a moderate activity against S. aureus (MIC > 128 g/ml) and low activity against E. coli (MIC = 512 g/ml). F10 CHCL3/MeOH did no present toxic activity against Artemia salina. The fractiorts F4 CHCL3/MeOH and Sf2 EtOAc/MeOH were toxic for this organism when the concentration was higher than 100 microg/ml. LC50 in both cases was 548.4 and 243.4 microg/ml respectively. Secondary metabolites of medium polarity obtained from A. fistularis have a wide spectrum of anti bacterial activity. Toxicity analysis suggests that only F10 CHCL3/MeOH has potential as an antimicrobial agent for clinical use. PMID:15266809

  11. Complete nucleotide sequence and genome organization of tobacco mosaic virus isolated from Viciafaba

    Institute of Scientific and Technical Information of China (English)

    周雪平; 薛朝阳; 陈青; 戚益军; 李德葆

    2000-01-01

    Based on reported TMV-U1 sequence, primers were designed and fragments covering the entire genome of TMV broad bean strain (TMV-B) were obtained with RT-PCR. These fragments were cloned and sequenced and the 5’ and 3’ end sequences of genome were confirmed with RACE. The complete sequence of TMV-B comprises 6 395 nucleotides (nt) and four open reading frames, which correspond to 126 ku (1 116 amino acids), 183 ku (1 616 amino acids), 30 ku (268 amino acids) and 17.5 ku proteins (159 amino acids). The complete nucleotide sequence of TMV-B is 99.4% identical to that of TMV-U1. The two virus isolates share the same sequence of 5’, 3’ non-coding region and 17.5 K ORF, and 6, 1 and 3 amino acid changes are found in 126 K protein, 54 K protein and 30 K protein, respectively. The possible mechanism on the infection of TMV-B in Vicia faba is discussed.

  12. Isolation of selenium organic species from antarctic krill after enzymatic hydrolysis

    Energy Technology Data Exchange (ETDEWEB)

    Siwek, Mariana; Galunsky, Boris [Technical University Hamburg-Harburg, Institute of Biotechnology II, Hamburg (Germany); Niemeyer, Bernd [GKSS Research Centre, Institute for Coastal Research, Geesthacht (Germany); University of the Federal Armed Forces Hamburg, Institute of Thermodynamics, Hamburg (Germany)

    2005-02-01

    Total selenium content and its distribution in the soluble and insoluble protein-bound fractions obtained after aqueous extraction of antarctic krill samples were determined. About 26% of the total selenium (2.4 {mu}g g{sup -1} dry weight) was found in the supernatant; the rest was in the pellet. Isolation of low molecular selenium-containing fractions was also performed by enzymatic digestion of the protein, followed by size-exclusion chromatography in conjunction with atomic absorption spectrometry. From the applied various proteinases (pronase E, subtilisin Carlsberg, trypsin, chymotrypsin, proteinase and proteinase N from Bacillus subtilis and Novo 0.6 MPX enzyme), the treatment with pronase E led to best recovery of selenium. About 96% of the total Se was found in the hydrolysate, mainly in low molecular weight fractions. Eighty percent of the Se species were in fractions with molecular weights in the range of amino acids and short peptides. High-performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC-ICP-MS) allowed the identification of selenomethionine and the assumption that selenocystine or its derivatives were the main species in these fractions. (orig.)

  13. Microbial selenite reduction with organic carbon and electrode as sole electron donor by a bacterium isolated from domestic wastewater.

    Science.gov (United States)

    Nguyen, Van Khanh; Park, Younghyun; Yu, Jaecheul; Lee, Taeho

    2016-07-01

    Selenium is said to be multifaceted element because it is essential at a low concentration but very toxic at an elevated level. For the purpose of screening a potential microorganism for selenite bioremediation, we isolated a bacterium, named strain THL1, which could perform both heterotrophic selenite reduction, using organic carbons such as acetate, lactate, propionate, and butyrate as electron donors under microaerobic condition, and electrotrophic selenite reduction, using an electrode polarized at -0.3V (vs. standard hydrogen electrode) as the sole electron donor under anaerobic condition. This bacterium determined to be a new strain of the genus Cronobacter, could remove selenite with an efficiency of up to 100%. This study is the first demonstration on a pure culture could take up electrons from an electrode to perform selenite reduction. The selenium nanoparticles produced by microbial selenite reduction might be considered for recovery and use in the nanotechnology industry. PMID:27099943

  14. High-pressure size exclusion chromatography analysis of dissolved organic matter isolated by tangential-flow ultra filtration

    Science.gov (United States)

    Everett, C.R.; Chin, Y.-P.; Aiken, G.R.

    1999-01-01

    A 1,000-Dalton tangential-flow ultrafiltration (TFUF) membrane was used to isolate dissolved organic matter (DOM) from several freshwater environments. The TFUF unit used in this study was able to completely retain a polystyrene sulfonate 1,800-Dalton standard. Unaltered and TFUF-fractionated DOM molecular weights were assayed by high-pressure size exclusion chromatography (HPSEC). The weight-averaged molecular weights of the retentates were larger than those of the raw water samples, whereas the filtrates were all significantly smaller and approximately the same size or smaller than the manufacturer-specified pore size of the membrane. Moreover, at 280 nm the molar absorptivity of the DOM retained by the ultrafilter is significantly larger than the material in the filtrate. This observation suggests that most of the chromophoric components are associated with the higher molecular weight fraction of the DOM pool. Multivalent metals in the aqueous matrix also affected the molecular weights of the DOM molecules. Typically, proton-exchanged DOM retentates were smaller than untreated samples. This TFUF system appears to be an effective means of isolating aquatic DOM by size, but the ultimate size of the retentates may be affected by the presence of metals and by configurational properties unique to the DOM phase.

  15. Antimicrobial activity of organic extracts isolated from Aplysina fistularis (Demospongiae: Aplysinidae)

    International Nuclear Information System (INIS)

    Organic extracts of the sponge Aplysina fistularis (Pallas 1766), were tested for antimicrobial activity against Gram positive bacteria (Staphylococcus aureus) and Gram negative bacteria (Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa). The minimal inhibitory concentration (MIC) and toxic activity of bioactive extract were determined. Susceptibility trials of organic fractions obtained by VLC. Hexane, EtOAc and CHCl3 showed that EtOAc fraction has antibacterial activity against E. coli, while CHCl3 fraction inhibited E. coli and S. aureus growth. The later refractioning of EtOAc fraction and the biodirected assays showed that fractions F12 and F13 of EtOAc/Hex and EtOAc F14 were bioactive against E. coli, K. pneumoniae and S. aureus. Only EtOAc/MeOH Sf2 from subfractioning of EtOAc F14 produced inhibition for E. coli and S. aureus. In Sf2 EtOAc/MeOH, MIC was moderate for S. aureus (MIC > 256 μg/ml). F4 CHCl3/MeOH produced a high inhibition in S. aureus (MIC = 0.125 μg/ml) and for E. coli (MIC > 16 μg/ml). F10 CHCl3/MeOH showed a moderate activity against S. aureus (MIC > 128 μg/ml) and low activity against E. coli (MIC = 512 μg/ml). F10 CHCI3/MeOH did no present toxic activity against Artemia salina. The fractiorts F4 CHCI3/MeOH and Sf2 EtOAc/MeOH were toxic for this organism when the concentration was higher than 100 μg/ml. LC50 in both cases was 548.4 and 243.4 μg/ml respectively. The conclusion is that secondary metabolites of medium polarity obtained from A. fistularis have a wide spectrum of anti bacterial activity. Toxicity analysis suggests that only F10 CHCI3/MeOH has potential as an antimicrobial agent for clinical use. (author)

  16. Isolation, purification and characterisation of an organic solvent-tolerant Ca2+-dependent protease from Bacillus megaterium AU02.

    Science.gov (United States)

    Priya, J Deepa Arul; Divakar, K; Prabha, M Suriya; Selvam, G Panneer; Gautam, Pennathur

    2014-01-01

    A new organic solvent-tolerant strain Bacillus megaterium AU02 which secretes an organic solvent-tolerant protease was isolated from milk industry waste. Statistical methods were employed to achieve optimum protease production of 43.6 U/ml in shake flask cultures. The productivity of the protease was increased to 53 U/ml when cultivated under controlled conditions in a 7-L fermentor. The protease was purified to homogeneity by a three-step process with 24 % yield and specific activity of 5,375 U/mg. The molecular mass of the protease was found to be 59 kDa. The enzyme was active over a wide range of pH (6.0–9.0), with an optimum activity at pH 7.0 and temperature from 40 to 70 °C having an optimum activity at 50 °C. The thermal stability of the enzyme increased significantly in the presence of CaCl2, and it retained 90 % activity at 50 °C for 3 h. The Km and Vmax values were determined as 0.722 mg/ml and 0.018 U/mg respectively. The metalloprotease exhibited significant stability in the presence of organic solvents with log P values more than 2.5, nonionic detergents and oxidising agent. An attempt was made to test the synthesis of aspartame precursor (Cbz-Asp-Phe-NH2) which was catalysed by AU02 protease in the presence of 50 % DMSO. These properties of AU02 protease make it an ideal choice for enzymatic peptide synthesis in organic media. PMID:24122712

  17. Isolation of a Halophilic Bacterium, Bacillus sp. Strain NY-6 for Organic Contaminants Removal in Saline Wastewater on Ship

    Institute of Scientific and Technical Information of China (English)

    Jie Gao; Zhenjiang Yu; Xiaohui Zhang; Dan Zhao; Fangbo Zhao

    2013-01-01

    The objective of this research was to examine if certain strains of Bacillus bacteria,could survive in dry powder products and if so,could the bacteria degrade organic contaminants in saline wastewater on a ship.As part of the study,we isolated 7 domesticated strains named NY1,NY2,…,and NY7,the strain NY6 showed to have the best performance for organic matter degradation and could survive in dry powder more than 3 months.NY6 was identified as Bacillus aerius,based on the morphological and physic-chemical properties.Its optimal growth conditions were as follows:salinity was 2%; temperature was 37℃; pH was in 6.5-7.0; best ratio of C∶ N∶ P was 100∶5∶1.The capability of its dry powder for Chemical Oxygen Demand (COD) removal was 800mg COD/g in synthesized marine wastewater with 2% salinity.The spores in the dry powder were 1.972× 108 g1.

  18. Optical properties of estuarine dissolved organic matter isolated using cross-flow ultrafiltration

    Institute of Scientific and Technical Information of China (English)

    YI Yueyuan; ZHENG Airong; GUO Weidong; YANG Liyang; CHEN Ding

    2014-01-01

    Dissolved organic matter (DOM) from freshwater, mid-salinity, and seawater endmember samples in the Jiulong River Estuary, China were fractionated using cross-flow ultrafiltration with a 10-kDa membrane. The colloidal organic matter (COM;10 kDa-0.22μm) retentate, low molecular weight (LMW ) DOM (<10 kDa) permeate, and bulk samples were analyzed using absorption spectroscopy and three-dimensional fluores-cence excitation-emission-matrix spectroscopy. The UV-visible spectra of COM were very similar to those obtained for permeate and bulk samples, decreasing monotonically with increasing wavelength. Most of the chromophoric DOM (CDOM, expressed as the absorption coefficient a355) occurred in the LMW fraction, while the percentage of CDOM in the colloidal fraction was substantially higher in the freshwater endmem-ber (13.4%of the total) than in the seawater endmember (6.8%). The bulk CDOM showed a conservative mixing behavior in the estuary, while there was removal of the COM fraction and a concurrent addition of the permeate fraction in the mid-salinity sample, implying that part of the colloidal CDOM was transformed into LMW CDOM. Two humic-like components (C1:<250, 325/402 nm;and C2:265, 360/458 nm) and one protein-like component (C3:275/334 nm) were identified using parallel factor analysis. The contributions of the C1, C2, and C3 components of the COM fraction to the bulk sample were 2.5%-8.7%, 4.8%-12.6%, and 7.4%-14.7%, respectively, revealing that fluorescent DOM occurred mainly in the LMW fraction in the Jiulong River Estuary. The C1 and C2 components in the retentate and permeate samples showed conserva-tive mixing behavior, but the intensity ratio of C2/C1 was higher in the retentate than in the permeate frac-tions for all salinity samples, showing that the humic component was more enriched in the COM than the fulvic component. The intensity ratio of C3/(C1+C2) was much higher in the retentate than in the permeate fraction for mid-salinity and seawater

  19. Identification and prospective isolation of a mesothelial precursor lineage giving rise to smooth muscle cells & fibroblasts for mammalian internal organs, and their vasculature

    OpenAIRE

    Rinkevich, Yuval; Mori, Taisuke; Sahoo, Debashis; Xu, Pin-Xian; Bermingham, John R., Jr.; Weissman, Irving L

    2012-01-01

    Fibroblasts and smooth muscle cells (FSMCs) are principal cell types of connective and adventitial tissues that participate in the development, physiology and pathology of internal organs, with incompletely defined cellular origins. Here, we identify and prospectively isolate from mesothelium a mouse cell lineage that is committed to FSMCs. Mesothelium is an epithelial monolayer covering the vertebrate thoracic and abdominal cavities and internal organs. Time-lapse imaging and transplantation...

  20. Introducing Organic Chemistry Students to Natural Product Isolation Using Steam Distillation and Liquid Phase Extraction of Thymol, Camphor, and Citral, Monoterpenes Sharing a Unified Biosynthetic Precursor

    Science.gov (United States)

    McLain, Katherine A.; Miller, Kenneth A.; Collins, William R.

    2015-01-01

    Plants have provided and continue to provide the inspiration and foundation for modern medicines. Natural product isolation is a key component of the process of drug discovery from plants. The purpose of this experiment is to introduce first semester undergraduate organic chemistry students, who have relatively few lab techniques at their…

  1. Implementation of the technique in isolated organ vascular as tool for the validation of medicinal plants: Study of the vasodilator effect of the S. scutellarioides

    Directory of Open Access Journals (Sweden)

    Jorge Hernán Ramírez

    2007-03-01

    Full Text Available Introduction: Salvia scutellarioides is a plant with antihypertensive and diuretic properties. In this study, its vasodilator effect is evaluated using the isolated organ technique.Objectives: To standardize the isolated vascular organ, and to study the effect of S. scutellarioides in vasoconstriction by a1 receptors stimulation.Methodology: Rat aortic rings were placed in an organ bath equipment (BIOPAC®, at 37o C temperature and constant carbogen gas bubbling. Tension changes were registered with an isometric tension transducer and data acquisition equipment. During the standardization, six curves of concentration-tension with phenylephrine at 1, 3 and 5 hours were registered, in order to determine maximum tension (TM, and the effective concentration 50 (EC50. Finally, three concentration-tension curves were obtained for phenylephrine with S. scutellarioides at a concentration of 10 mg/dl and 20 mg/dl. Results: During the technique standardization, no statistically significant differences were found in TM for phenylephrine at 1, 3 and 5 hours. The administration of S. scutellarioides produced a TM dose dependent reduction with no EC50 changes.Discussion: The implemented isolated organ technique is viable up to 5 hours, and does not produce a1 receptors desensibilization. The TM diminution by S. scutellarioides is explained by a non competitive a1 antagonism effect. Studies to isolate active principles for allowing the initiation of phase I human clinical trials are required.

  2. Isolates of Sarcocystis falcatula-like organisms from South American opossums Didelphis marsupialis and Didelphis albiventris from São Paulo, Brazil.

    Science.gov (United States)

    Dubey, J P; Lindsay, D S; Rosenthal, B M; Kerber, C E; Kasai, N; Pena, H F; Kwok, O C; Shen, S K; Gennari, S M

    2001-12-01

    Isolates of Sarcocystis falcatula-like organisms from South American opossums were characterized based on biological and morphological criteria. Sporocysts from intestinal scrapings of 1 Didelphis marsupialis and 8 Didelphis albiventris from São Paulo, Brazil, were fed to captive budgerigars (Melopsittacus undulatus). Budgerigars fed sporocysts from all 9 isolates became ill and S. falcatula-like schizonts were identified in sections of their lungs by immunohistochemical staining. Sarcocystis falcatula-like organisms were cultured from lungs of budgerigars fed sporocysts from D. marsupialis and from lungs of budgerigars fed sporocysts from 3 of 8 D. albiventris. The 33/54 locus amplified by polymerase chain reaction from culture-derived merozoites contained both a HinfI endonuclease recognition site previously suggested to diagnose S. falcatula and a DraI site thought to diagnosed S. neurona. Development of the isolate from D. marsupialis was studied in cell culture; its schizonts divided by endopolygeny, leaving a residual body. Morphological and genetic variation differentiated this Sarcocystis isolate originating in D. marsupialis from the Cornell I isolate of S. falcatula. This is the first report of a S. falcatula infection in the South American opossum, D. marsupialis. PMID:11780836

  3. Short communication: Prevalence of methicillin resistance in coagulase-negative staphylococci and Staphylococcus aureus isolated from bulk milk on organic and conventional dairy farms in the United States.

    Science.gov (United States)

    Cicconi-Hogan, K M; Belomestnykh, N; Gamroth, M; Ruegg, P L; Tikofsky, L; Schukken, Y H

    2014-05-01

    The objective of this study was to evaluate the presence of methicillin-resistant Staphylococcus aureus and coagulase-negative Staphylococcus spp. in bulk tank milk samples from 288 organic and conventional dairy farms located in New York, Wisconsin, and Oregon from March 2009 to May 2011. Due to recent publications reporting the presence mecC (a mecA homolog not detected by traditional mecA-based PCR methods), a combination of genotypic and phenotypic approaches was used to enhance the recovery of methicillin-resistant organisms from bulk tank milk. In total, 13 isolates were identified as methicillin resistant: Staph. aureus (n=1), Staphylococcus sciuri (n=5), Staphylococcus chromogenes (n=2), Staphylococcus saprophyticus (n=3), Staphylococcus agnetis (n=1), and Macrococcus caseolyticus (n=1). The single methicillin-resistant Staph. aureus isolate was identified from an organic farm in New York, for an observed 0.3% prevalence at the farm level. The methicillin-resistant coagulase-negative staphylococci prevalence was 2% in the organic population and 5% in the conventional population. We did not identify mecC in any of the isolates from our population. Of interest was the relatively high number of methicillin-resistant Staph. sciuri recovered, as the number of isolates from our study was considerably higher than those recovered from other recent studies that also assessed milk samples. Our research suggests that the presence of a potential methicillin-resistant Staphylococcus reservoir in milk, and likely the dairy farm population in the United States, is independent of the organic or conventional production system. PMID:24582450

  4. In vitro activity of dalbavancin and five comparator agents against common and uncommon Gram-positive organisms isolated from cancer patients.

    Science.gov (United States)

    Rolston, Kenneth V I; Wang, Weiqun; Nesher, Lior; Shelburne, Samuel A; Prince, Randall A

    2016-05-01

    Dalbavancin is a long acting, bactericidal lipoglycopeptide. Its in vitro activity was compared with that of vancomycin, daptomycin, linezolid, trimethoprim/sulfamethoxazole (TMP/SMX) and levofloxacin against 241 Gram-positive organisms isolated from cancer patients. The rank order of potency for the glycopeptides based on MIC90 (μg ml(-1)), that is, the concentration of antimicrobial agent required to inhibit 90% of isolates tested was dalbavancin (0.12 μg ml(-1))>daptomycin (1.0 μg ml(-1))>vancomycin (2.0 μg ml(-1)) for coagulase-negative staphylococci and Staphylococcus aureus isolates (including methicillin-susceptible (MSSA) and methicillin-resistant (MRSA) strains). Dalbavancin had potent activity against staphylococcal isolates with vancomycin MICs⩾1.0 μg ml(-1). TMP/SMX also had potent activity against staphylococci including methicillin-resistant strains, whereas levofloxacin had moderate to poor anti-staphylococcal activity. Dalbavancin also exhibited more potent activity than vancomycin and daptomycin against Bacillus spp., Corynebacterium spp., Micrococcus spp. and various streptococci (including Streptococcus pneumoniae, viridans group streptococci (VGS), beta-hemolytic streptococci and gamma-hemolytic streptococci). MBC determinations showed that dalbavancin had potent bactericidal activity against MRSA with no tolerance being detected. These data suggest that dalbavancin may be considered as an alternative to vancomycin, especially in institutions wherein a substantial proportion of infections are caused by organisms with vancomycin MICs⩾1.0 μg ml(-1). PMID:26626876

  5. DNA isolation from teeth by organic extraction and identification of sex of the individual by analyzing the AMEL gene marker using PCR

    OpenAIRE

    Subramanian Thangaraj Praveen Kumar; Nalini Aswath

    2016-01-01

    Background: To identify the sex of the deceased individual from dental hard tissue such as enamel and dentine. Objective: To isolate the DNA from dental hard tissue (enamel and dentin) from teeth extracted for prophylactic purpose, to assess the quality and purity of DNA and to identify the sex using polymerized chain reactor (PCR). Materials and Methods: DNA was extracted following phenol/chloroform (organic) extraction from 20 male and 20 female teeth. The samples that contain the amelogeni...

  6. Genome organization of the Kresse strain of porcine parvovirus: identification of the allotropic determinant and comparison with those of NADL-2 and field isolates.

    OpenAIRE

    Bergeron, J.; Hébert, B; Tijssen, P.

    1996-01-01

    The Kresse strain of porcine parvovirus (PPV) was cloned into pUC19, and independent infectious clones were sequenced. The PPV Kresse and NADL-2 strains, which have different pathogenicities, shared an identical genomic organization and a high degree of sequence identity. Partial genomes (1.5 or 1.6 kb) of 15 field isolates were also amplified by PCR in regions with significant sequence differences between the laboratory strains. Five amino acid differences were consistently present within th...

  7. Complete Detoxification of Short Chain Chlorinated Aliphatic Compounds: Isolation of Halorespiring Organisms and Biochemical Studies of the Dehalogenating Enzyme Systems - Final Report; FINAL

    International Nuclear Information System (INIS)

    Work focused on the isolation and characterization of halorespiring populations, and the initial investigation of the dechlorinating enzyme systems. In addition, tools to evaluate the presence/activity to halorespiring populations in the environment were developed. The tools developed in this work (measurements of hydrogen consumption thresholds, molecular probes) are relevant for regulatory agencies in order to facilitate decisions on which bioremediation technology (biostimulation or bioaugmentation) is most promising at a particular site. In addition, a better understanding of the physiology of the halorespiring organisms as well as the biochemistry of the dehalogenating enzyme systems enhances our knowledge of how these organisms can successfully be employed in the bioremediation of contaminated sites

  8. Complete Detoxification of Short Chain Chlorinated Aliphatic Compounds: Isolation of Halorespiring Organisms and Biochemical Studies of the Dehalogenating Enzyme Systems - Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Tiedje, J.M.

    1999-10-01

    Work focused on the isolation and characterization of halorespiring populations, and the initial investigation of the dechlorinating enzyme systems. In addition, tools to evaluate the presence/activity to halorespiring populations in the environment were developed. The tools developed in this work (measurements of hydrogen consumption thresholds, molecular probes) are relevant for regulatory agencies in order to facilitate decisions on which bioremediation technology (biostimulation or bioaugmentation) is most promising at a particular site. In addition, a better understanding of the physiology of the halorespiring organisms as well as the biochemistry of the dehalogenating enzyme systems enhances our knowledge of how these organisms can successfully be employed in the bioremediation of contaminated sites.

  9. Resistance to penicillin of Staphylococcus aureus isolates from cows with high somatic cell counts in organic and conventional dairy herds in Denmark

    Directory of Open Access Journals (Sweden)

    Vaarst Mette

    2006-11-01

    Full Text Available Abstract Background Quarter milk samples from cows with high risk of intramammary infection were examined to determine the prevalence of Staphylococcus aureus (SA and penicillin resistant SA (SAr in conventional and organic dairy herds and herds converting to organic farming in a combined longitudinal and cross-sectional study. Methods 20 conventional herds, 18 organic herds that converted before 1995, and 19 herds converting to organic farming in 1999 or 2000 were included in the study. Herds converting to organic farming were sampled three times one year apart; the other herds were sampled once. Risk of infection was estimated based on somatic cell count, milk production, breed, age and lactation stage. Results The high-risk cows represented about 49 % of the cows in the herds. The overall prevalence of SA and SAr among these cows was 29% (95% confidence interval: 24%–34% and 4% (95% confidence interval: 2%–5% respectively. The prevalence of penicillin resistance among SA infected cows was 12% (95% confidence interval: 6%–19% when calculated from the first herd visits. No statistically significant differences were observed in the prevalence of SAr or the proportion of isolates resistant to penicillin between herd groups. Conclusion The proportion of isolates resistant to penicillin was low compared to studies in other countries except Norway and Sweden. Based on the low prevalence of penicillin resistance of SA, penicillin should still be the first choice of antimicrobial agent for treatment of bovine intramammary infection in Denmark.

  10. Characterization and copper binding of humic and nonhumic organic matter isolated from the South Platte River: Evidence for the presence of nitrogenous binding site

    Science.gov (United States)

    Croue, J.-P.; Benedetti, M.F.; Violleau, D.; Leenheer, J.A.

    2003-01-01

    Humic substances typically constitute 40-60% of the dissolved organic matter (DOM) in surface waters. However, little information is available regarding the metal binding properties of the nonhumic hydrophilic portion of the DOM. In this study, humic and nonhumic DOM samples were isolated from the South Platte River (Colorado, DOC = 2.6 mg??L-1, SUVA254 = 2.4 L/mg??m) using a two-column array of XAD-8 and XAD-4 resins. The three major isolated fractions of DOM, which accounted for 57% of the bulk DOM, were characterized using a variety of analytical tools. Proton and copper binding properties were studied for each fraction. The main objective of this work was to compare the structural and chemical characteristics of the isolated fractions and test models describing DOM reactivity toward metal ions. The characterization work showed significant structural differences between the three isolated fractions of DOM. The hydrophobic acid fraction (i.e., humic substances isolated from the XAD-8 resin) gave the largest C/H, C/O, and C/N ratios and aromatic carbon content among the three isolated fractions. The transphilic acid (TPHA) fraction ("transphilic" meaning fraction of intermediate polarity isolated from the XAD-4 resin) was found to incorporate the highest proportion of polysaccharides, whereas the transphilic neutral (TPHN) fraction was almost entirely proteinaceous. The gradual increase of the charge with pH for the three DOM fractions is most likely caused by a large distribution of proton affinity constants for the carboxylic groups, as well as a second type of group more generally considered to be phenolic. In the case of the DOM fraction enriched in proteinaceous material (i.e., TPHN fraction), the results showed that the amino groups are reponsible for the charge reversal. For low copper concentrations, nitrogen-containing functional groups similar to those of amino acids are likely to be involved in complexation, in agreement with previously published data.

  11. Solubilization of inorganic phosphate and production of organic acids by bacteria isolated from a Moroccan mineral phosphate deposit

    OpenAIRE

    Mardad, Illham; Serrano, Aurelio; Soukri, Abdelaziz

    2013-01-01

    Three efficient inorganic-phosphate solubilizing bacteria (PSB) were isolated from a phosphate rock deposit of a Moroccan mine. The phosphate solubilization index of these isolates, determined in National Botanical Research Institute's phosphate (NBRIP) medium supplemented with tribasic calcium phosphate, ranging from 2.8 to 4.4. The medium pH dropped from 7.0 to 3.5 units after growth under continuous agitation for seven days. PSB6, the most efficient PSB, closely related to Enterobacter hor...

  12. Isolation of pure Babesia equi and Babesia caballi organisms in splenectomized horses from endemic areas in South Africa.

    Science.gov (United States)

    De Waal, D T; Van Heerden, J; Van den Berg, S S; Stegmann, G F; Potgieter, F T

    1988-03-01

    Both Babesia equi and Babesia caballi are endemic in large parts of South Africa. Attempts were made to obtain pure local isolates of both B. equi and B. caballi for the purpose of developing serological tests to study the epidemiology of equine babesiosis in this country. The indirect fluorescent antibody test was used to screen horses for B. equi and B. caballi in an endemic area. Seven horses and 3 donkeys between 3 and 36 months of age that tested negative were subsequently splenectomized. The splenectomy operation was performed through the abdominal approach. A 100% survival rate was achieved through this method, probably because it reduced the risk involved in the operation. Blood collected from naturally infected horses and passaged in fully susceptible splenectomized horses and a donkey, under laboratory conditions, produced 2 isolates of Babesia caballi and 1 of B. equi. Microscopical and serological examinations confirmed that these were pure isolates. PMID:3353098

  13. Contribution of effluent organic matter (EfOM) to ultrafiltration (UF) membrane fouling: Isolation, characterization, and fouling effect of EfOM fractions

    KAUST Repository

    Zheng, Xing

    2014-11-01

    EfOM has been regarded as a major organic foulant resulting in UF membrane fouling in wastewater reclamation. To investigate fouling potential of different EfOM fractions, the present study isolated EfOM into hydrophobic neutrals (HPO-N), colloids, hydrophobic acids (HPO-A), transphilic neutrals and acids (TPI), and hydrophilics (HPI), and tested their fouling effect in both salt solution and pure water during ultrafiltration (UF). Major functional groups and chemical structure of the isolates were identified using Fourier transform infrared spectroscopy (FT-IR) and solid-state carbon nuclear magnetic resonance (13C NMR) analysis. The influence of the isolation process on the properties of EfOM fractions was minor because the raw and reconstituted secondary effluents were found similar with respect to UV absorbance, molecular size distribution, and fluorescence character. In membrane filtration tests, unified membrane fouling index (UMFI) and hydraulic resistance were used to quantify irreversible fouling potential of different water samples. Results show that under similar DOC level in feed water, colloids present much more irreversible fouling than other fractions. The fouling effect of the isolates is related to their size, chemical properties, and solution chemistry. Further investigations have identified that the interaction between colloids and other fractions also influences the performance of colloids in fouling phenomena. © 2014 Elsevier Ltd.

  14. On the nature of organic matter from natural and contaminated materials : isolation methods, characterisation and application to geochemical modelling

    NARCIS (Netherlands)

    Zomeren, van A.

    2008-01-01

    Natural organic matter (NOM) is the material that is formed after the natural decomposition and transformation of dead plant and animal matter. The fresh organic matter (e.g. plant leaves or animal debris) is decomposed and transformed by microbial activity. As such, NOM is found everywhere in th

  15. UTILIZATION OF ORGANIC NITROGEN-SOURCES BY 2 PHYTOPLANKTON SPECIES AND A BACTERIAL ISOLATE IN PURE AND MIXED CULTURES

    NARCIS (Netherlands)

    IETSWAART, T; SCHNEIDER, PJ; PRINS, RA

    1994-01-01

    Algal production of dissolved organic carbon and the regeneration of nutrients from dissolved organic carbon by bacteria are important aspects of nutrient cycling in the sea, especially when inorganic nitrogen is limiting. Dissolved free amino acids are a major carbon source for bacteria and can be

  16. Organizations

    DEFF Research Database (Denmark)

    Hatch, Mary Jo

    Most of us recognize that organizations are everywhere. You meet them on every street corner in the form of families and shops, study in them, work for them, buy from them, pay taxes to them. But have you given much thought to where they came from, what they are today, and what they might become in...... considers many more. Mary Jo Hatch introduces the concept of organizations by presenting definitions and ideas drawn from the a variety of subject areas including the physical sciences, economics, sociology, psychology, anthropology, literature, and the visual and performing arts. Drawing on examples from...... prehistory and everyday life, from the animal kingdom as well as from business, government, and other formal organizations, Hatch provides a lively and thought provoking introduction to the process of organization....

  17. Induction of arthritis with Mycoplasma hyosynoviae in pigs: Clinical response and re-isolation of the organism from body fluids and organs

    DEFF Research Database (Denmark)

    Hagedorn-Olsen, T.; Nielsen, N.C.; Friis, N.F.

    1999-01-01

    mycoplasma is cultivable from synovial fluids and other tissues. In this article we report on the clinical response to infection and the results obtained from re-isolation attempts. In three inoculation experiments with M. hyosynoviae, clinical arthritis was produced by intravenous and by intranasal exposure...... half of the cases the onset was acute and the lameness severe, Epically involving the hindlegs and with affected pigs assuming a dog-sitting position. A systemic phase was found in the majority (86 %) of the pigs. However, the infection was in two cases established in the tonsils without detection of a...

  18. PHOTOGENERATION OF SINGLET OXYGEN AND FREE RADICALS IN DISSOLVED ORGANIC MATTER ISOLATED FROM THE MISSISSIPPI AND ATCHAFALAYA RIVER PLUMES

    Science.gov (United States)

    The photoreactivity to UV light of ultrafiltered dissolved organic matter (DOM) collected during cruises along salinity transects in the Mississippi and Atchafalaya River plumes was examined by measuring photogenerated free radicals and singlet molecular oxygen (1O2) photosensiti...

  19. On the nature of organic matter from natural and contaminated materials : isolation methods, characterisation and application to geochemical modelling

    OpenAIRE

    Zomeren, van, A.

    2008-01-01

    Natural organic matter (NOM) is the material that is formed after the natural decomposition and transformation of dead plant and animal matter. The fresh organic matter (e.g. plant leaves or animal debris) is decomposed and transformed by microbial activity. As such, NOM is found everywhere in the natural environment; in soils, surface water and oceans. Due to its abundance at the earth’s surface, the production and decomposition of NOM plays an important role in the global carbon cycling. In...

  20. Prevalence and diversity of Arcobacter spp. isolated from the internal organs of spontaneous porcine abortions in Denmark

    DEFF Research Database (Denmark)

    On, Stephen L.W.; Jensen, Tim Kåre; Bille-Hansen, Vivi; Jorsal, Sven Erik Lind; Vandamme, P.

    2002-01-01

    aborted fetuses from a single sow. The high prevalence of arcobacters in Danish pig abortions may account for at least some of the >90% of cases in which no established abortifacient agent is detected, but further studies are needed to define the role of each species, especially where co-infections with......A study was conducted to determine the prevalence and possible significance of campylobacteria in pig abortions in Denmark, Surface-cauterised liver and kidney samples from 55 aborted pig fetuses submitted to the Danish Veterinary Laboratory were taken and a sensitive isolation procedure used to...

  1. Purification and characterization of novel organic solvent tolerant 98kDa alkaline protease from isolated Stenotrophomonas maltophilia strain SK.

    Science.gov (United States)

    Waghmare, Shailesh R; Gurav, Aparna A; Mali, Sonal A; Nadaf, Naiem H; Jadhav, Deepak B; Sonawane, Kailas D

    2015-03-01

    Ability of microorganisms to grow at alkaline pH makes them an attractive target for several industrial applications. Thus, search for new extremozyme producing microorganisms must be a continuous exercise. Hence, we isolated a potent alkaline protease producing bacteria from slaughter house soil. The morphological, biochemical and 16S rDNA gene sequencing studies revealed that the isolated bacteria is Stenotrophomonas maltophilia strain SK. Alkaline protease from S. maltophilia strain SK was purified by using ammonium sulphate precipitation and DEAE-cellulose ion exchange column chromatography. The purified enzyme was optimally active at pH 9.0 and temperature 40°C with broad substrate specificity. It was observed that the metal ions such as Ca(++), Mg(++) and Fe(+++) completely repressed the enzyme activity. The enzyme was stable in presence of various water miscible solvents like ethanol, methanol, isopropanol at 25% (v/v) concentration and less stable at 37.5% (v/v) concentration. These robust properties of enzyme might be applicable for various applications in detergent and pharmaceutical industries. PMID:25462807

  2. Formation of brominated disinfection byproducts from natural organic matter isolates and model compounds in a sulfate radical-based oxidation process

    KAUST Repository

    Wang, Yuru

    2014-12-16

    A sulfate radical-based advanced oxidation process (SR-AOP) has received increasing application interest for the removal of water/wastewater contaminants. However, limited knowledge is available on its side effects. This study investigated the side effects in terms of the production of total organic bromine (TOBr) and brominated disinfection byproducts (Br-DBPs) in the presence of bromide ion and organic matter in water. Sulfate radical was generated by heterogeneous catalytic activation of peroxymonosulfate. Isolated natural organic matter (NOM) fractions as well as low molecular weight (LMW) compounds were used as model organic matter. Considerable amounts of TOBr were produced by SR-AOP, where bromoform (TBM) and dibromoacetic acid (DBAA) were identified as dominant Br-DBPs. In general, SR-AOP favored the formation of DBAA, which is quite distinct from bromination with HOBr/OBr- (more TBM production). SR-AOP experimental results indicate that bromine incorporation is distributed among both hydrophobic and hydrophilic NOM fractions. Studies on model precursors reveal that LMW acids are reactive TBM precursors (citric acid > succinic acid > pyruvic acid > maleic acid). High DBAA formation from citric acid, aspartic acid, and asparagine was observed; meanwhile aspartic acid and asparagine were the major precursors of dibromoacetonitrile and dibromoacetamide, respectively.

  3. Isolation of dissolved organic matter from permafrost soil and freshwater environments of the Kolyma River basin, east Siberia, for high resolution structural analysis

    Science.gov (United States)

    Dubinenkov, I. V.; Perminova, I. V.; Bulygina, E. B.; Holmes, R. M.; Davydov, S.; Mann, P. J.; Vonk, J.; Zimov, S. A.

    2010-12-01

    The Arctic and Subarctic ecosystems are known to be the most vulnerable with respect to climate change. Hence, research on carbon cycling in the Arctic region is very important for understanding the current climatic trends and their consequences. The Kolyma River watershed is one of the Arctic Ocean’s largest. It is dominated by continuous permafrost which is underlain with rich organic soils susceptible to increased fluvial transport. The thaw of permafrost enhanced due to global warming might provide additional large source of organic carbon to the Kolyma River and to the Arctic Ocean as a whole. For estimating the contribution of this source to the total pool of organic carbon, specific structural features of permafrost dissolved organic matter (DOM) as opposed to the waterborne DOM of the Kolyma River should be identified and monitored. The objective of this work was to isolate a representive set of the DOM samples from permafrost soil and freshwater environments of the Kolyma River basin suitable for further structural analysis using high resolution Fourier Transform Ion Cyclotron Resonance Mass Spectroscopy (FTICR-MS) and 1H NMR spectroscopy. The isolation protocol of DOM used in this study has been developed by Dittmar et al, 2008 for sampling marine DOM for NMR studies. It is based on the solid phase extraction of DOM from seawater using PPL Varian Bond Elute cartridges Those cartridges were shown to possess the highest efficiency in DOM isolation from marine water. Prior to discharge through the cartridge, a water sample was filtered through 0.45 μm filter for separation of particulate matter and acidified to pH 2 using HCl. About 50mg of DOM could be sequestered from aqueous phase using one cartridge. Sorption extent was monitored by measurements of DOC concentration and UV-vis spectra at the inlet and outlet of the cartridge. It was determined that from 60 to 65% of the total DOC could be extracted from the tested samples of freshwater. As a result

  4. Isolation and characterization of nanosheets containing few layers of the Aurivillius family of oxides and metal-organic compounds

    Energy Technology Data Exchange (ETDEWEB)

    Sreedhara, M.B.; Prasad, B.E.; Moirangthem, Monali [Chemistry and Physics of Materials Unit, New Chemistry Unit, International Centre for Materials Science and Sheik Saqr Laboratory, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur P.O., Bangalore 560064 (India); Murugavel, R. [Department of Chemistry, Indian Institute of Technology–Bombay, Powai, Mumbai 400076 (India); Rao, C.N.R., E-mail: cnrrao@jncasr.ac.in [Chemistry and Physics of Materials Unit, New Chemistry Unit, International Centre for Materials Science and Sheik Saqr Laboratory, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur P.O., Bangalore 560064 (India)

    2015-04-15

    Nanosheets containing few-layers of ferroelectric Aurivillius family of oxides, Bi{sub 2}A{sub n−1}B{sub n}O{sub 3n+3} (where A=Bi{sup 3+}, Ba{sup 2+} etc. and B=Ti{sup 4+}, Fe{sup 3+} etc.) with n=3, 4, 5, 6 and 7 have been prepared by reaction with n-butyllithium, followed by exfoliation in water. The few-layer samples have been characterized by Tyndall cones, atomic force microscopy, optical spectroscopy and other techniques. The few-layer species have a thickness corresponding to a fraction of the c-parameter along which axis the perovskite layers are stacked. Magnetization measurements have been carried out on the few-layer samples containing iron. Few-layer species of a few layered metal-organic compounds have been obtained by ultrasonication and characterized by Tyndall cones, atomic force microscopy, optical spectroscopy and magnetic measurements. Significant changes in the optical spectra and magnetic properties are found in the few-layer species compared to the bulk samples. Few-layer species of the Aurivillius family of oxides may find uses as thin layer dielectrics in photovoltaics and other applications. - Graphical abstract: Exfoliation of the layered Aurivillius oxides into few-layer nanosheets by chemical Li intercalation using n-BuLi followed by reaction in water. Exfoliation of the layered metal-organic compounds into few-layer nanosheets by ultrasonication. - Highlights: • Few-layer nanosheets of Aurivillius family of oxides with perovskite layers have been generated by lithium intercalation. • Few-layer nanosheets of few layered metal-organic compounds have been generated by ultrasonication. • Few-layer nanosheets of the Aurivillius oxides have been characterized by AFM, TEM and optical spectroscopy. • Aurivillius oxides containing Fe show layer dependent magnetic properties. • Exfoliated few-layer metal-organic compounds show changes in spectroscopic and magnetic properties compared with bulk materials.

  5. Isolation and characterization of nanosheets containing few layers of the Aurivillius family of oxides and metal-organic compounds

    International Nuclear Information System (INIS)

    Nanosheets containing few-layers of ferroelectric Aurivillius family of oxides, Bi2An−1BnO3n+3 (where A=Bi3+, Ba2+ etc. and B=Ti4+, Fe3+ etc.) with n=3, 4, 5, 6 and 7 have been prepared by reaction with n-butyllithium, followed by exfoliation in water. The few-layer samples have been characterized by Tyndall cones, atomic force microscopy, optical spectroscopy and other techniques. The few-layer species have a thickness corresponding to a fraction of the c-parameter along which axis the perovskite layers are stacked. Magnetization measurements have been carried out on the few-layer samples containing iron. Few-layer species of a few layered metal-organic compounds have been obtained by ultrasonication and characterized by Tyndall cones, atomic force microscopy, optical spectroscopy and magnetic measurements. Significant changes in the optical spectra and magnetic properties are found in the few-layer species compared to the bulk samples. Few-layer species of the Aurivillius family of oxides may find uses as thin layer dielectrics in photovoltaics and other applications. - Graphical abstract: Exfoliation of the layered Aurivillius oxides into few-layer nanosheets by chemical Li intercalation using n-BuLi followed by reaction in water. Exfoliation of the layered metal-organic compounds into few-layer nanosheets by ultrasonication. - Highlights: • Few-layer nanosheets of Aurivillius family of oxides with perovskite layers have been generated by lithium intercalation. • Few-layer nanosheets of few layered metal-organic compounds have been generated by ultrasonication. • Few-layer nanosheets of the Aurivillius oxides have been characterized by AFM, TEM and optical spectroscopy. • Aurivillius oxides containing Fe show layer dependent magnetic properties. • Exfoliated few-layer metal-organic compounds show changes in spectroscopic and magnetic properties compared with bulk materials

  6. Medium-based optimization of an organic solvent-tolerant extracellular lipase from the isolated halophilic Alkalibacillus salilacus.

    Science.gov (United States)

    Samaei-Nouroozi, Amene; Rezaei, Shahla; Khoshnevis, Nika; Doosti, Mahmoud; Hajihoseini, Reza; Khoshayand, Mohammad Reza; Faramarzi, Mohammad Ali

    2015-09-01

    A haloalkaliphilic solvent-tolerant lipase was produced from Alkalibacillus salilacus within 48 h of growth in liquid medium. An overall 4.9-fold enhanced production was achieved over unoptimized media after medium optimization by statistical approaches. Plackett-Burman screening suggested lipase production maximally influenced by olive oil, KH2PO4, NaCl, and glucose; and response surface methodology predicted the appropriate levels of each parameter. Produced lipase was highly active and stable over broad ranges of temperature (15-65 °C), pH (4.0-11.0), and NaCl concentration (0-30 %) showing excellent thermostable, pH-stable, and halophilic properties. The enzyme was optimally active at pH 8.0 and 40 °C. Majority of cations, except some like Co(2+) and Al(3+) were positive signals for lipase activity. In addition, the presence of chemical agents and organic solvents with different log P ow was well tolerated by the enzyme. Finally, efficacy of lipase-mediated esterification of various alcohols with oleic acid in organic solvents was studied. PMID:26198037

  7. Isolation of Pythium oligandrum from Egyptian soil and its mycoparasitic effect on Pythium ultimum var. ultimum the damping-off organism of wheat.

    Science.gov (United States)

    Abdelzaher, H M; Elnaghy, M A; Fadl-Allah, E M

    1997-01-01

    Pythium oligandrum Drechsler bearing spherical sporangia with complex subglobose elements was isolated for the first time in Egypt from agricultural field soil cultivated with alfalfa (Trifolium alexandrinum) in El-Minia, Egypt. This fungus was found to be an active bio-control agent against P. ultimum var. ultimum, the damping-off organism of wheat. In agar plates, P. oligandrum parasitized P. ultimum var. ultimum hyphae with the aid of thin haustorial branches or infection pegs, eventually leading to host destruction. Incorporation of P. oligandrum into carboxymethylcellulose seed coating successfully eliminated pre-emergence damping-off of wheat caused by P. ultimum var. ultimum, whereas Post-emergence damping-off was prevented by adding inocula of P. oligandrum to the soil. PMID:16284719

  8. Biodegradation of azaarenes and creosote in aqueous and organic liquid phase immobilized cell bioreactors by bacteria isolated from creosote contaminated soil

    International Nuclear Information System (INIS)

    The biodegradation of azaarenes and coal-tar creosote was studied using aerobic bacteria isolated from creosote contaminated soil as inocula in batch cultures and in immobilized cell bioreactors. Biodegradation of quinoline, isoquinoline, and 6-methylquinoline by pure and mixed cultures yielded mono-hydroxylated metabolites as the primary products of azaarene metabolism. All azaarene degrading cultures could degrade quinoline, suggesting a common metabolic pathway based on quinoline metabolism. Mixed cultures attacking creosote degraded 2- and 3-ring polyaromatic hydrocarbons and heterocycles, but were unable to degrade 4- and 5-ring PAH. The degradation rate and loading capacity for quinoline was greatly enhanced in the bioreactors in comparison to batch cultures. The rates of isoquinoline, 6-methylquinoline degrading strain of Pseudomonas putida successfully removed 6-methylquinoline from solution in decane in a water-limited, non-aqueous liquid phase immobilized cell bioreactor. These experiments demonstrate the ability of environmental organisms to biodegrade several biologically active compounds under conditions suitable for bioremediation applications

  9. An organic solvent-stable lipase from a newly isolated Staphylococcus aureus ALA1 strain with potential for use as an industrial biocatalyst.

    Science.gov (United States)

    Ben Bacha, Abir; Moubayed, Nadine Ms; Al-Assaf, Alaa

    2016-05-01

    In this study, a new strain, ALA1, was identified as Staphylococcus aureus by biochemical tests, and its 16S ribosomal DNA sequence was isolated from dromedary milk. ALA1 lipase production was optimized in shake flask experiments and measured with varying pH (3-11), temperature (20-55 °C) and substrate concentrations. The maximum lipase production was recorded at pH 8 and 30 °C for up to 30 H of culture period for the S. aureus ALA1 strain. Among the substrates tested, selected carbon sources, xylose, nitrogen source, yeast extract, and olive oil (1%) were suitable for maximizing lipase production. The effects of surfactants were investigated and showed that Tween 20, Tween 80, and Triton X-100 prevented lipase production. Interestingly, isolate ALA1 was able to grow in high concentrations of benzene or toluene (up to 50% (v/v)). Moreover, the lipolytic activity of the S. aureus ALA1 lipase was stimulated by diethyl ether, whereas almost 100% of S. aureus ALA1 lipase activity was retained in 25% acetone, acetonitrile, benzene, 2-propanol, ethanol, methanol, or toluene. Because of its stability in organic solvent, the S. aureus ALA1 lipase was used as a biocatalyst to synthesize high levels of added value molecules. S. aureus ALA1 lipase could be considered as an ideal choice for applications in detergent formulations because of its high stability and compatibility with various surfactants, oxidizing agents, and commercial detergents. PMID:25828848

  10. Characterization and Function of the First Antibiotic Isolated from a Vent Organism: The Extremophile Metazoan Alvinella pompejana

    Science.gov (United States)

    Tasiemski, Aurélie; Jung, Sascha; Boidin-Wichlacz, Céline; Jollivet, Didier; Cuvillier-Hot, Virginie; Pradillon, Florence; Vetriani, Costantino; Hecht, Oliver; Sönnichsen, Frank D.; Gelhaus, Christoph; Hung, Chien-Wen; Tholey, Andreas; Leippe, Matthias; Grötzinger, Joachim; Gaill, Françoise

    2014-01-01

    The emblematic hydrothermal worm Alvinella pompejana is one of the most thermo tolerant animal known on Earth. It relies on a symbiotic association offering a unique opportunity to discover biochemical adaptations that allow animals to thrive in such a hostile habitat. Here, by studying the Pompeii worm, we report on the discovery of the first antibiotic peptide from a deep-sea organism, namely alvinellacin. After purification and peptide sequencing, both the gene and the peptide tertiary structures were elucidated. As epibionts are not cultivated so far and because of lethal decompression effects upon Alvinella sampling, we developed shipboard biological assays to demonstrate that in addition to act in the first line of defense against microbial invasion, alvinellacin shapes and controls the worm's epibiotic microflora. Our results provide insights into the nature of an abyssal antimicrobial peptide (AMP) and into the manner in which an extremophile eukaryote uses it to interact with the particular microbial community of the hydrothermal vent ecosystem. Unlike earlier studies done on hydrothermal vents that all focused on the microbial side of the symbiosis, our work gives a view of this interaction from the host side. PMID:24776651

  11. Characterization and function of the first antibiotic isolated from a vent organism: the extremophile metazoan Alvinella pompejana.

    Directory of Open Access Journals (Sweden)

    Aurélie Tasiemski

    Full Text Available The emblematic hydrothermal worm Alvinella pompejana is one of the most thermo tolerant animal known on Earth. It relies on a symbiotic association offering a unique opportunity to discover biochemical adaptations that allow animals to thrive in such a hostile habitat. Here, by studying the Pompeii worm, we report on the discovery of the first antibiotic peptide from a deep-sea organism, namely alvinellacin. After purification and peptide sequencing, both the gene and the peptide tertiary structures were elucidated. As epibionts are not cultivated so far and because of lethal decompression effects upon Alvinella sampling, we developed shipboard biological assays to demonstrate that in addition to act in the first line of defense against microbial invasion, alvinellacin shapes and controls the worm's epibiotic microflora. Our results provide insights into the nature of an abyssal antimicrobial peptide (AMP and into the manner in which an extremophile eukaryote uses it to interact with the particular microbial community of the hydrothermal vent ecosystem. Unlike earlier studies done on hydrothermal vents that all focused on the microbial side of the symbiosis, our work gives a view of this interaction from the host side.

  12. The Identification of Complex Organic Molecules in the Interstellar Medium: Using Lasers and Matrix Isolation Spectroscopy to Simulate the Interstellar Environment

    Science.gov (United States)

    Stone, Bradley M.

    1998-01-01

    The Astrochemistry Group at NASA Ames Research Center is interested in the identification of large organic molecules in the interstellar medium Many smaller organic species (e.g. hydrocarbons, alcohols, etc.) have been previously identified by their radiofrequency signature due to molecular rotations. However, this becomes increasingly difficult to observe as the size of the molecule increases. Our group in interested in the identification of the carriers of the Diffuse Interstellar Bands (absorption features observed throughout the visible and near-infrared in the spectra of stars, due to species in the interstellar medium). Polycyclic Aromatic Hydrocarbons (PAHs) and related molecules are thought to be good candidates for these carriers. Laboratory experiments am performed at Ames to simulate the interstellar environment, and to compare spectra obtained from molecules in the laboratory to those derived astronomically. We are also interested in PAHs with respect to their possible connection to the UIR (Unidentified infrared) and ERE (Extended Red Emission) bands - emission features found to emanate from particular regions of our galaxy (e.g. Orion nebula, Red Rectangle, etc.). An old, "tried and proven spectroscopic technique, matrix isolation spectroscopy creates molecular conditions ideal for performing laboratory astrophysics.

  13. PRODUCTION AND CHARACTERIZATION OF AN ALKALOTHERMOSTABLE, ORGANIC SOLVENT TOLERANT AND SURFACTANT TOLERANT ESTERASE PRODUCED BY A THERMOPHILIC BACTERIUM GEOBACILLUS SP. AGP-04, ISOLATED FROM BAKRESHWAR HOT SPRING, INDIA

    Directory of Open Access Journals (Sweden)

    Amit Ghati

    2013-10-01

    Full Text Available A thermophilic bacteria, Geobacillus sp. AGP-04, isolated from Surya Kund hot spring, Bakreshwar, West Bengal, India was studied in terms of capability of tributyrin hydrolysis and characterization of its thermostable esterase activity using p-nitrophenyl butyrate (PNPB as substrate. The extracellular crude preparation was characterized in terms of pH and temperature optima and stability, organic solvent tolerance capacity and stability, substrate specificity, surfactant tolerance capacity, kinetic parameters and activation/inhibition behavior towards some metal ions and chemicals. Tributyrin agar assay exhibited that Geobacillus sp. AGP-04 secretes an extracellular esterase. The Vmax and Km values of the esterase were found to be 5099 U/Land 103.5µM, respectively in the presence of PNPB as substrate. The optimum temperature and pH, for Geobacillus sp. AGP-04 esterase was 60oC and 8.0, respectively. Although the enzyme activity was not significantly altered by incubating crude extract solution at 20-70oC for 1 hour, the enzyme activity was fully lost at 90oC for same incubation period. The pH stability profile showed that original crude esterase activity is stable at a broad range (pH 5.0-10.0. Moreover, the enzyme was highly organic solvent and surfactant tolerant. The effect of some chemical on crude esterase activity indicated that Geobacillus sp. AGP-04 produce an esterase which contains a serine residue in active site and for its activity -SH groups are essential. Besides, enzyme production was highly induced if fermentation medium contain polysaccharides and oil as carbon source.

  14. DNA isolation from teeth by organic extraction and identification of sex of the individual by analyzing the AMEL gene marker using PCR

    Directory of Open Access Journals (Sweden)

    Subramanian Thangaraj Praveen Kumar

    2016-01-01

    Full Text Available Background: To identify the sex of the deceased individual from dental hard tissue such as enamel and dentine. Objective: To isolate the DNA from dental hard tissue (enamel and dentin from teeth extracted for prophylactic purpose, to assess the quality and purity of DNA and to identify the sex using polymerized chain reactor (PCR. Materials and Methods: DNA was extracted following phenol/chloroform (organic extraction from 20 male and 20 female teeth. The samples that contain the amelogenin gene (amel were amplified by PCR. The products of the PCR were run on agarose gel with ethidium bromide staining on gel documentation system. Results: The results on the gel showed the presence of X-specific bands at 212 bp and Y-specific bands at 218 bp. Males were distinguished from females by the presence of two bands whereas female samples showed only one, that is, X-specific band on the gel. The gender from the known samples was determined with complete accuracy, and the results were analyzed statistically by the Chi-square test. Conclusion: In our study, the PCR-based method showed 100% specificity and sensitivity.

  15. LIFE experiment: isolation of cryptoendolithic organisms from Antarctic colonized sandstone exposed to space and simulated Mars conditions on the international space station.

    Science.gov (United States)

    Scalzi, Giuliano; Selbmann, Laura; Zucconi, Laura; Rabbow, Elke; Horneck, Gerda; Albertano, Patrizia; Onofri, Silvano

    2012-06-01

    Desiccated Antarctic rocks colonized by cryptoendolithic communities were exposed on the International Space Station (ISS) to space and simulated Mars conditions (LiFE-Lichens and Fungi Experiment). After 1.5 years in space samples were retrieved, rehydrated and spread on different culture media. Colonies of a green alga and a pink-coloured fungus developed on Malt-Agar medium; they were isolated from a sample exposed to simulated Mars conditions beneath a 0.1 % T Suprasil neutral density filter and from a sample exposed to space vacuum without solar radiation exposure, respectively. None of the other flight samples showed any growth after incubation. The two organisms able to grow were identified at genus level by Small SubUnit (SSU) and Internal Transcribed Spacer (ITS) rDNA sequencing as Stichococcus sp. (green alga) and Acarospora sp. (lichenized fungal genus) respectively. The data in the present study provide experimental information on the possibility of eukaryotic life transfer from one planet to another by means of rocks and of survival in Mars environment. PMID:22688852

  16. Ceftolozane/tazobactam activity tested against aerobic Gram-negative organisms isolated from intra-abdominal and urinary tract infections in European and United States hospitals (2012).

    Science.gov (United States)

    Sader, Helio S; Farrell, David J; Flamm, Robert K; Jones, Ronald N

    2014-09-01

    Ceftolozane/tazobactam is under clinical development for treatment of complicated intra-abdominal infections (IAI), complicated urinary tract infections (UTI) and ventilator-associated pneumonia. We evaluated the in vitro activity of ceftolozane/tazobactam and comparator agents tested against Gram-negative aerobic bacteria causing IAI and healthcare-associated UTI (HCA-UTI). The organisms were consecutively collected from January to December 2012 from 59 medical centers located in the United States (USA) and 15 European countries by the Program to Assess Ceftolozane/Tazobactam Susceptibility (PACTS). The collection included 809 organisms from IAI and 2474 organisms from HCA-UTI, and susceptibility testing was performed by reference broth microdilution methods as described by the Clinical and Laboratory Standards Institute (CLSI) M07-A9 document. Overall, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa were the most frequently isolated pathogens from both infection types. Ceftolozane/tazobactam was very active against E. coli (MIC50/90, 0.25/0.5 mg/L; 98.5-99.9% inhibited at an MIC of ≤8 mg/L) and retained activity against many of the multidrug-resistant (MDR; MIC50/90, 0.5/2->32 mg/L) and ESBL-phenotype strains (MIC50/90, 0.5/2-32 mg/L). Ceftolozane/tazobactam was active against most K. pneumoniae strains (MIC50/90, 0.25/16 mg/L, 88.9-89.6% inhibited at an MIC of ≤8 mg/L), but some ESBL-phenotype (MIC50/90, 4-8/>32 mg/L) and MDR (MIC50/90, 16/>32 mg/L) isolates exhibited elevated MIC values. Ceftolozane/tazobactam was the most active agent tested against P. aeruginosa (MIC50/90, 0.5/4 mg/L; 93.4-95.7% inhibited at ≤8 mg/L) and retained potency against many MDR (MIC50/90, 2-4/>32 mg/L), ceftazidime-nonsusceptible (MIC50/90, 2-4/>32 mg/L) and meropenem-nonsusceptible (MIC50/90, 2/>32 mg/L) strains. Ceftolozane/tazobactam was also active against Klebsiella oxytoca (MIC50/90, ≤0.12-0.25/0.5-1 mg/L), Enterobacter spp. (MIC50/90, 0

  17. Analysis of Plant Growth-Promoting Effects of Fluorescent Pseudomonas Strains Isolated from Mentha piperita Rhizosphere and Effects of Their Volatile Organic Compounds on Essential Oil Composition.

    Science.gov (United States)

    Santoro, Maricel V; Bogino, Pablo C; Nocelli, Natalia; Cappellari, Lorena Del Rosario; Giordano, Walter F; Banchio, Erika

    2016-01-01

    Many species or strains of the genus Pseudomonas have been characterized as plant growth promoting rhizobacteria (PGPR). We used a combination of phenotypic and genotypic techniques to analyze the community of fluorescent Pseudomonas strains in the rhizosphere of commercially grown Mentha piperita (peppermint). Biochemical techniques, Amplified rDNA Restriction Analysis (ARDRA), and 16S rRNA gene sequence analysis revealed that the majority of the isolated native fluorescent strains were P. putida. Use of two Repetitive Sequence-based PCR (rep-PCR) techniques, BOX-PCR and ERIC-PCR, allowed us to evaluate diversity among the native strains and to more effectively distinguish among them. PGPR activity was tested for the native strains and reference strain P. fluorescens WCS417r. Micropropagated M. piperita plantlets were exposed to microbial volatile organic compounds (mVOCs) emitted by the bacterial strains, and plant biomass parameters and production of essential oils (EOs) were measured. mVOCs from 11 of the native strains caused an increase in shoot fresh weight. mVOCs from three native strains (SJ04, SJ25, SJ48) induced changes in M. pierita EO composition. The mVOCs caused a reduction of metabolites in the monoterpene pathway, for example menthofuran, and an increase in menthol production. Menthol production is the primary indicator of EO quality. The mVOCs produced by native strains SJ04, SJ25, SJ48, and strain WCS417r were analyzed. The obtained mVOC chromatographic profiles were unique for each of the three native strains analyzed, containing varying hydrocarbon, aromatic, and alogenic compounds. The differential effects of the strains were most likely due to the specific mixtures of mVOCs emitted by each strain, suggesting a synergistic effect occurs among the compounds present. PMID:27486441

  18. Analysis of Plant Growth-Promoting Effects of Fluorescent Pseudomonas Strains Isolated from Mentha piperita Rhizosphere and Effects of Their Volatile Organic Compounds on Essential Oil Composition

    Science.gov (United States)

    Santoro, Maricel V.; Bogino, Pablo C.; Nocelli, Natalia; Cappellari, Lorena del Rosario; Giordano, Walter F.; Banchio, Erika

    2016-01-01

    Many species or strains of the genus Pseudomonas have been characterized as plant growth promoting rhizobacteria (PGPR). We used a combination of phenotypic and genotypic techniques to analyze the community of fluorescent Pseudomonas strains in the rhizosphere of commercially grown Mentha piperita (peppermint). Biochemical techniques, Amplified rDNA Restriction Analysis (ARDRA), and 16S rRNA gene sequence analysis revealed that the majority of the isolated native fluorescent strains were P. putida. Use of two Repetitive Sequence-based PCR (rep-PCR) techniques, BOX-PCR and ERIC-PCR, allowed us to evaluate diversity among the native strains and to more effectively distinguish among them. PGPR activity was tested for the native strains and reference strain P. fluorescens WCS417r. Micropropagated M. piperita plantlets were exposed to microbial volatile organic compounds (mVOCs) emitted by the bacterial strains, and plant biomass parameters and production of essential oils (EOs) were measured. mVOCs from 11 of the native strains caused an increase in shoot fresh weight. mVOCs from three native strains (SJ04, SJ25, SJ48) induced changes in M. pierita EO composition. The mVOCs caused a reduction of metabolites in the monoterpene pathway, for example menthofuran, and an increase in menthol production. Menthol production is the primary indicator of EO quality. The mVOCs produced by native strains SJ04, SJ25, SJ48, and strain WCS417r were analyzed. The obtained mVOC chromatographic profiles were unique for each of the three native strains analyzed, containing varying hydrocarbon, aromatic, and alogenic compounds. The differential effects of the strains were most likely due to the specific mixtures of mVOCs emitted by each strain, suggesting a synergistic effect occurs among the compounds present. PMID:27486441

  19. Isolation of Stem rot Disease Causing Organism of Brinjal and their in-vitro Inhibition with Fungicides and Bio-control Agents

    Directory of Open Access Journals (Sweden)

    Shaily Javeria

    2014-09-01

    Full Text Available Different strains of Sclerotinia sclerotiorum were isolated from the diseased samples collected from different hosts and locations. Among the 14 isolates, 12 isolates colonies covered the entire Petri plates within 96 hours but, two isolates from fababean and yellow mustard showed slow colony growth within 96 hours. All isolates produced sclerotia which were varied in number, but the fenugreek isolate produced maximum (43 number of sclerotia and lambs quarter isolate produced minimum number of sclerotia (12 on PDA medium. To examine inhibitory effect of fungicide on the mycelial growth of the pathogen, 9 fungicides were tested in vitro against Sclerotinia sclerotiorum, of those carbendazim, carboxin, topsin-M and carbendazim+ mancozeb (SAAF were found most effective and inhibited the mycelial growth of pathogen up to 100 per cent at 0.05%, 0.1%, and 0.2% concentration. The effect of different bioagents viz., Trichoderma harzianum, T. viride, T. koningii, T. atroviride, T. longibraciatum, Aspergillus niger, Chaetomium globosome and Penicillium notatum in inhibiting the growth of Sclerotinia sclerotiorum was studied through “Dual Culture Technique”. The data showed that among the eight biocontrol agent six were fond effective. The maximum inhibition was found by T. harzianum causing 70.82% inhibition of mycelial growth of the pathogen S. sclerotiorum.

  20. Isolation and characterization of antimicrobial proteins produced by a potential probiotic strain of human Lactobacillus rhamnosus 231 and its effect on selected human pathogens and food spoilage organisms

    OpenAIRE

    Ambalam, P. S.; Prajapati, J. B.; Dave, J. M.; Nair, Baboo M.; Ljungh, Åsa; Vyas, B. R. M.

    2011-01-01

    Objective: To study in vitro properties of potential probiotics and the antimicrobial activity of Lactobacillus rhamnosus 231 isolated from human faeces. Methods and Results: Lact. rhamnosus 231 isolated from human faeces tolerated bile salt (4%), phenol (0.5%), and NaCl (4%) and retained viability at low pH (2.5). The cell-free culture (CFC) fi ltrate and extracellular protein concentrate (EPC) of Lact. rhamnosus 231 contained antimicrobial substances active against Pseudomonas aeruginosa, E...

  1. Effect of Lactobacillus sp. isolates supernatant on Escherichia coli O157:H7 enhances the role of organic acids production as a factor for pathogen control

    Directory of Open Access Journals (Sweden)

    Larissa B. Poppi

    2015-04-01

    Full Text Available Many attempts have been made to establish the control of foodborne pathogens through Lactobacillus isolates and their metabolism products with success being obtained in several situations. The aim of this study was to investigate the antagonistic effect of eight Lactobacillus isolates, including L. casei subsp. pseudoplantarum, L. plantarum, L. reuteri and L. delbrueckii subsp. delbrueckii, on the pathogenic Escherichia colistrain O157:H7. The inhibitory effect of pure cultures and two pooled cultures supernatants of Lactobacillus on the growth of pathogenic bacteria was evaluated by the spot agar method and by monitoring turbidity. Antimicrobial activity was confirmed for L. reuteri and L. delbrueckii subsp. delbrueckii and for a pool of lactic acid bacteria. The neutralized supernatant of the pool exerted a higher antimicrobial activity than that of the individual strains. Furthermore, D-lactic acid and acetic acid were produced during growth of the Lactobacillus isolates studied.

  2. [Comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (1995). III. Differences in susceptibilities from previous years].

    Science.gov (United States)

    Kumamoto, Y; Tsukamoto, T; Hirose, T; Yokoo, A; Hikichi, Y; Shigeta, S; Takahashi, T; Shiraiwa, Y; Ogiwara, M; Yoshida, H; Imafuku, Y; Murai, M; Watanabe, K; Uchida, H; Kobayashi, Y; Matsuda, S; Fujime, M; Fujita, K; Igari, J; Oguri, T; Kosakai, N; Yamaguchi, K; Matsumoto, T; Kashitani, F; Mochida, C

    1997-04-01

    Susceptibilities to various antimicrobial agents were examined for Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Klebsiella spp. and Pseudomonas aeruginosa that were isolated from patients with urinary tract infections (UTIs) in 11 hospitals during June, 1995 through May, 1996, and the results were compared with those obtained during the same period in earlier years. 1. Macrolide resistant E. faecalis isolated from uncomplicated UTIs during the latest study period appeared to have increased compared to those in previous study periods. More than 50% of the isolated E. faecalis during the latest study period were resistant to macrolide antibiotics, for the first time in our history. 2. No obvious changes were observed through the years for susceptibilities of S. aureus to various antimicrobial agents. Vancomycin (VCM) showed the highest activity against S. aureus, with MICs below 2 micrograms/ml or below. 3. Among E. coli strains, those with low susceptibilities to quinolones appeared to have increased over the years with MIC90 changed from between 0.125 microgram/ml or below and 0.5 microgram/ml in the 1989-1990 period to between 8 micrograms/ml and 128 micrograms/ml in the latest study period. 4. Klebsiella spp. showed higher resistance to most antimicrobial agents during periods of 1993-1994 and 1994-1995, but somewhat lower resistance during period of 1995-1996. No resistant Klebsiella spp. were detected from uncomplicated UTIs during the latest study period. 5. Among P. aeruginosa isolates from complicated UTIs, resistance isolates to gentamicin appeared to be increasing over the years. Resistant strains to quinolones were isolated at lower frequencies during periods of 1991-1994, but higher frequency was observed in the latest period, and MIC50s were between 0.5 and 4 micrograms/ml during 1991-1994, but were 16-32 micrograms/ml during 1995-1996. These susceptibility changes should be utilized in determining clinical treatments. PMID:9192248

  3. Resistance to antimicrobial agents in campylobacter isolated from chickens raised in intensive and organic farms and its implications for the management of risk to human health

    OpenAIRE

    Soonthornchaikul, Nantika

    2006-01-01

    The use of antimicrobials in poultry may lead to the emergence of resistant micro-organisms that could cause additional health risk to humans through food consumption. This study aims to investigate the relative health risks from Campylobacter and its antimicrobial resistance associated with chicken raised in organic and intensive rearing systems. Three groups of chicken were tested, pre-packaged intensively reared (PIC) and pre-packaged organically reared chickens (POC) both purchased fro...

  4. Potential influence of organic compounds on the transport of radionuclides from a geologic repository. Assessment of effectiveness of geologic isolation systems

    International Nuclear Information System (INIS)

    This study identifies organic compounds that may be present in a repository and outlines plausible interactions and mechanisms that may influence the forms and chemical behavior of these compounds. A review of the literature indicates that large quantities of organic radioactive wastes are generated by the nuclear industry and if placed in a repository could increase or decrease the leach rate and sorption characteristics of waste radionuclides. The association of radionuclides with organic matter can render the nuclides soluble or insoluble depending on the particular nuclide and such parameters as the pH, Eh, and temperature of the hydrogeologic system as well as the properties of the organic compounds themselves. 44 references

  5. Ca2+influx insensitive to organic Ca2+entry blockers contributes to noradrenaline-induced contractions of the isolated guinea pig aorta

    NARCIS (Netherlands)

    Gouw, M.A.M.; Wilffert, B.; Wermelskirchen, D.; Van Zwieten, P.A.

    1990-01-01

    We determined the contribution of intracellular Ca2+to the noradrenaline (NA, 3 x 10-5mmol/l)-induced contraction of the isolated guinea pig aorta. Since only about 55% of the NA-induced contraction could be attributed to intracellular Ca2+release, we assumed that a Ca2+influx component contributes

  6. Similarities in the genome organization of tobacco rattle virus and pea early-browning virus isolates that are transmitted by the same vector nematode.

    Science.gov (United States)

    MacFarlane, S A; Vassilakos, N; Brown, D J

    1999-01-01

    Although sequence data have been obtained for several tobravirus isolates, only two of these isolates are nematode-transmissible. Tobacco rattle virus (TRV) PpK20 is transmitted by Paratrichodorus pachydermus, whereas pea early-browning virus (PEBV) TpA56 is transmitted by Trichodorus primitivus. To clarify whether differences in the genome structure of these isolates are relevant to the specificity of interactions with particular vector nematodes, or merely reflect a taxonomic difference between TRV and PEBV, we have sequenced RNA2 of a new isolate of TRV (TpO1) that is transmitted by the same vector nematode as PEBV TpA56 but is not transmitted by the nematode vector of TRV PpK20. TRV TpO1 RNA2 encodes, in 5' to 3' order, a coat protein (CP), a 9K protein, a 2b (29K) protein and a 2c (18K) protein. Amino acid sequence comparison shows that both the CP and 2b proteins of TRV TpO1 resemble more closely the analogous proteins from PEBV TpA56 than those from TRV PpK20. Also, the TRV TpO1 9K protein has similarities with the PEBV 9K protein whereas this protein is lacking in TRV PpK20. PMID:9934712

  7. Draft Genome Sequences of Psychrobacter Strains JCM 18900, JCM 18901, JCM 18902, and JCM 18903, Isolated Preferentially from Frozen Aquatic Organisms

    OpenAIRE

    Kudo, Toshiaki; Kidera, Akihiro; Kida, Muneaki; Kawauchi, Ayumi; Shimizu, Ryo; Nakahara, Tomomi; Zhang, Xiaochi; Yamada, Akinori; Amano, Masao; Hamada, Yuki; Taniyama, Shigeto; Arakawa, Osamu; Yoshida, Asami; Oshima, Kenshiro; Suda, Wataru

    2014-01-01

    Four Psychrobacter strains, JCM 18900, JCM 18901, JCM 18902, and JCM 18903, related to either Psychrobacter nivimaris or Psychrobacter cibarius, were isolated from frozen marine animals. The genome information of these four strains will be useful for studies of their physiology and adaptation properties to frozen conditions.

  8. 论“信息孤岛”对政府组织发展的制约与对策--基于学习型组织理论的视角%The Information Isolated Island Restrictions to the Development of Governmental Organizations and Its Solutions:On the Perspective of Learning Organization Theory

    Institute of Scientific and Technical Information of China (English)

    唐平秋; 蒋晓飞

    2015-01-01

    “信息孤岛”作为我国信息化社会发展的重要障碍,正制约着政府组织的发展。本文根据不同标准对政府组织中的信息孤岛进行了分类,借鉴学习型组织理论中系统思考的方法,运用“成长上限”基模对政府组织的发展问题进行分析,探讨了“信息孤岛”对政府组织发展的制约作用,并提出了打破政府组织中信息孤岛的具体措施。%The information isolated island is a great barrier to the development of the information -based society,con-stantly restricting the development of governmental organizations.This article classifies the information isolated island of the governmental organizations,on the basis of different standards.It applies the systematic thinking method of learning organization theory and the fundamental model of limits to growth to analyzing the restriction effect of the information isolated island on the development of the governmental organizations,and brings up some practical solutions.

  9. [Comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (1996), III. Secular changes in susceptibility].

    Science.gov (United States)

    Kumamoto, Y; Tsukamoto, T; Hirose, T; Yokoo, A; Shigeta, S; Takahashi, T; Shiraiwa, Y; Ogiwara, M; Yoshida, H; Imafuku, Y; Murai, M; Watanabe, K; Uchida, H; Kobayashi, Y; Matsuda, S; Fujime, M; Fujita, K; Igari, J; Oguri, T; Yamaguchi, K; Matsumoto, T; Kashitani, F; Kaku, M; Ooe, H; Mochida, C

    1998-03-01

    Susceptibilities to various antimicrobial agents were examined for Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Klebsiella spp., and Pseudomonas aeruginosa that were isolated from patients with urinary tract infections (UTIs) in 10 hospitals during June 1996 to May 1997, and the results were compared with those obtained during the same period in earlier years. 1. E. faecalis Among E. faecalis strains, those with high susceptibilities to ampicillin and minocycline appeared to have decreased in the latest study period. 2. S. aureus To almost antimicrobial agents, S. aureus isolated from uncomplicated UTIs showed low susceptibilities. But the MIC50s of those agents for S. aureus from complicated UTIs have changed better state. Particularly, the MIC50s of imipenem and clindamycin were 0.125 microgram/ml or below in the latest period for the first time in our history. 3. E. coli The susceptibilities to piperacillin and quinolones of E. coli isolated from uncomplicated UTIs were better than those isolated from complicated UTIs. 4. Klebsiella spp. The susceptibilities to almost antimicrobial agents of Klebsiella spp. have been better during the latest period, compared to those during period of 1995-1996, but to ofloxacin and ciprofloxacin have appeared to have been lower. 5. P. aeruginosa The susceptibilities to quinolones of P. aeruginosa have been better during the latest period compared those during periods of 1995-1996. But, the susceptibilities to cefozopran, carbapenems and monobactams of P. aeruginosa isolated from complicated UTIs appeared to have been lower. These susceptibility changes should be utilized in determining clinical treatments. PMID:9597551

  10. Co-permeability of 3H-labeled water and 14C-labeled organic acids across isolated plant cuticles. Investigating cuticular paths of diffusion and predicting cuticular transpiration

    International Nuclear Information System (INIS)

    Penetration of 3H-labeled water (3H2O) and the 14C-labeled organic acids benzoic acid ([14C]BA), salicylic acid ([14C]SA), and 2,4-dichlorophenoxyacetic acid ([14C]2,4-D) were measured simultaneously in isolated cuticular membranes of Prunus laurocerasus L., Ginkgo biloba L., and Juglans regia L. For each of the three pairs of compounds (3H2O/[14C]BA, 3H2O/[14C]SA, and 3H2O/[14C]2,4-D) rates of cuticular water penetration were highly correlated with the rates of penetration of the organic acids. Therefore, water and organic acids penetrated the cuticles by the same routes. With the combination 3H2O/[14C]BA, co-permeability was measured with isolated cuticles of nine other plant species. Permeances of 3H2O of all 12 investigated species were highly correlated with the permeances of [14C]BA (r2 = 0.95). Thus, cuticular transpiration can be predicted from BA permeance. The application of this experimental method, together with the established prediction equation, offers the opportunity to answer several important questions about cuticular transport physiology in future investigations

  11. [Comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (1998). III. Secular changes in susceptibility].

    Science.gov (United States)

    Kumamoto, Y; Tsukamoto, T; Hirose, T; Matsukawa, M; Kunishima, Y; Matsuda, S; Sato, S; Ogiwara, M; Ishibashi, K; Fujime, M; Fujita, K; Shigeta, S; Watanabe, J; Igari, J; Yoshida, H; Imafuku, Y; Oguri, T; Murai, M; Yamaguchi, K; Matsumoto, T; Kashitani, F; Watanabe, K; Kobayashi, Y; Uchida, H; Ooe, H; Nishikawa, M; Kohno, S; Oka, T; Takano, Y; Miyagawa, Y; Miyazaki, Y; Izumikawa, K; Yamaguchi, T; Mochida, C; Furuhama, T

    2000-05-01

    Susceptibilities to various antimicrobial agents were examined for Enterococcus faecalis, Staphylococcus aureus, Echerichia coli, Klebsiella spp. and Pseudomonas aeruginosa that were isolated from patients with urinary tract infections (UTIs) in 9 hospitals during June 1998 to May 1999, and the results were compared with those obtained during the same period from 1990 to 1997 in uncomplicated UTIs and complicated UTIs. Among E. faecalis strains, those with low susceptibilities to almost drugs have increased in the latest period. All 5 S. aureus strains isolated from uncomplicated UTIs were the most susceptible to gentamicin (GM). Over 50% of S. aureus strains isolated from complicated UTIs were susceptible to GM, and on the contrary the resistant strains have increased with the MIC90 of 256 micrograms/ml or above. Among S. aureus strains isolated from complicated UTIs, those with low susceptibilities to arbekacin (ABK) have increased in the latest period compared to those during period of 1996-1997, and the MIC90s of them have changed into the lower state from 1 microgram/ml in 1996-1997 to 4 micrograms/ml in 1998. S. aureus strains have continued high susceptibilities to vancomycin (VCM). The susceptibilities to minocycline (MINO) of E. coli showed MIC90: 4 micrograms/ml in 1997, but those have returned in the latest period in uncomplicated UTIs. The MIC90s of ofloxacin (OFLX) to E. coli isolated from uncomplicated and complicated UTIs have been lower 2-3 classes in the latest period than those in 1997. Among Klebsiella spp. strains isolated from uncomplicated UTIs, those with low susceptibilities to almost cephems had increased in 1997, but few of them were detected in the latest study. The sensitive strains of P. aeruginosa to almost drugs have increased during the latest period. The MIC50s of cefozopran (CZOP) and OFLX against P. aeruginosa were the best in our history. The sensitive strains of P. aeruginosa to ceftazidime (CAZ) have increased and its

  12. Testing the biocompatibility of a glutathione-containing intra-ocular irrigation solution by using an isolated perfused bovine retina organ culture model - an alternative to animal testing.

    Science.gov (United States)

    Januschowski, Kai; Zhour, Ahmad; Lee, Albert; Maddani, Ramin; Mueller, Sebastien; Spitzer, Martin S; Schnichels, Sven; Schultheiss, Maximilian; Doycheva, Deshka; Bartz-Schmidt, Karl-Ulrich; Szurman, Peter

    2012-03-01

    The effects of a glutathione-containing intra-ocular irrigation solution, BSS Plus©, on retinal function and on the survival of ganglion cells in whole-mount retinal explants were studied. Evidence is provided that the perfused ex vivo bovine retina can serve as an alternative to in vivo animal testing. Isolated bovine retinas were prepared and perfused with an oxygen-saturated standard irrigation solution, and an electroretinogram was recorded to assess retinal function. After stable b-waves were detected, the isolated retinas were perfused with BSS Plus for 45 minutes. To investigate the effects of BSS Plus on photoreceptor function, 1mM aspartate was added to the irrigation solution in order to obtain a-waves, and the ERG trace was monitored for 75 minutes. For histological analysis, isolated whole retinal mounts were stored for 24 hours at 4°C, in the dark. The percentages of cell death in the retinal ganglion cell layer and in the outer and inner nuclear layers were estimated by using an ethidium homodimer-1 stain and the TUNEL assay. General swelling of the retina was examined with high-resolution optical coherence tomography. During perfusion with BSS Plus, no significant changes in a-wave and b-wave amplitudes were recorded. Retinas stored for 24 hours in BSS Plus showed a statistically significant smaller percentage (52.6%, standard deviation [SD] = 16.1%) of cell death in the retinal ganglion cell layer compared to the control group (69.6%, SD = 3.9, p = 0.0031). BSS Plus did not seem to affect short-term retinal function, and had a beneficial effect on the survival of retinal ganglion cells. This method for analysing the isolated perfused retina represents a valuable alternative for testing substances for their retinal biocompatibility and toxicity. PMID:22558975

  13. Bioavailability of the sodium pertechnetate and morphometry of organs isolated from rats: study of possible pharmacokinetic interactions of a ginkgo biloba extract

    International Nuclear Information System (INIS)

    Many compounds affect the bioavailability of radiobiocomplexes as radiopharmaceuticals. Ginkgo Biloba extract (EGb) has several effects. The influence of an EGb on the bioavailability of the radiobiocomplex sodium pertechnetate (Na99m TcO4) and on the morphometry of the organs was evaluated. Rats were treated with EGb and Na99m TcO4 was injected. The animals were sacrificed; the radioactivity in the organs was counted. The results showed that EGb altered the Na99m TcO4 bioavailability in the kidneys, liver and duodenum. Morphometric analysis of the organs showed significant alterations (P99m TcO4. (author)

  14. Partial purification and characterization of the organic solvent-tolerant lipase produced by Pseudomonas fluorescens RB02-3 isolated from milk.

    Science.gov (United States)

    Boran, Rukiye; Ugur, Aysel

    2010-01-01

    Eighty-five putative Pseudomonas isolates were obtained from various raw milk and pasteurized milk samples using Pseudomonas CFC agar. Among them, 36 isolates were identified as Pseudomonas fluorescens, and one isolate was identified as Pseudomonas putida. Lipase activity of the strains was quantitatively measured by the spectrophotometric method using p-nitrophenyl palmitate (p-NPP) as substrate. Detected lipase activity of the strains was between 10.03 U/mL and 22.16 U/mL. Pseudomonas fluorescens RB02-3 possessed the highest lipase activity. The extracellular lipase of P. fluorescens RB02-3 strain was homogeneously purified using a combination of ammonium sulfate precipitation, dialysis, and gel filtration column chromatography. This purification procedure resulted in 2.97-fold purification with 20.3% recovery. The enzyme was characterized, and exhibited maximum activity at pH 7.0 and 50 °C; after it was incubated for 1 h it was activated in the presence of hexane, ethyl acetate, isopropanol, and ethanol and remained stable after the incubation was extended for 2 hr. The lipase was slightly inhibited in the presence of Zn²+, Co²+, Cu²+, Ni²+ salts, and ethylenediamine tetraacetic acid (EDTA), whereas Cd²+, sodium dodecyl sulfate (SDS), and Tween-80 had no effect on its activity. PMID:21108127

  15. Tribological study of hard coatings without cobalt intended to isolation components of PWR primary cooling system; Etude tribologique de revetements durs sans cobalt destines aux organes d`isolement du circuit primaire des REP

    Energy Technology Data Exchange (ETDEWEB)

    Cachon, L.

    1995-10-18

    The objective is to qualify coatings without cobalt to replace ``Stellites`` coatings in isolation valves of PWR primary cooling system, as Co is activated when passing in the reactor core and contaminated the cooling loop. Three families of coatings were tested: PVD thin films from 1 to 8 {mu}m monolayers of Cr/C{sub x} with x varying between 1.6 and 9.5 at% or multilayers of pure chromium and Cr/C{sub 1.6} at%, coatings with a thickness between 100 and 200 {mu}m of cermets NiCr{sub y} (y varying from 5 to 35 at%) matrix binding chromium or tungsten carbides, and thick coatings 2 mm thickness of cermets Nitronic 60 or Inconel 625 matrix binding 10, 20 or 30% titanium or niobium carbides. Stellite 6 (2 mm) is the reference coating for tribology. Coatings were qualified and selected by thermal shocks, corrosion and plane friction. The thin film and the thick families were disqualified by their destruction or by their high friction coefficient. Then coatings between 100 and 200 {mu}m were used in a valve mock-up working in PWR primary cooling system pressure and temperature conditions. Tests show that these coatings have better wear or tightness performances than stellite 6, except for a slightly higher friction coefficient. (A.B.).

  16. Promoter isolation and characterization of GhAO-like1, a Gossypium hirsutum gene similar to multicopper oxidases that is highly expressed in reproductive organs.

    Science.gov (United States)

    Lambret-Frotté, Julia; Artico, Sinara; Muniz Nardeli, Sarah; Fonseca, Fernando; Brilhante Oliveira-Neto, Osmundo; Grossi-de-Sá, Maria Fatima; Alves-Ferreira, Marcio

    2016-01-01

    Cotton is one of the most economically important cultivated crops. It is the major source of natural fiber for the textile industry and an important target for genetic modification for both biotic stress and herbicide tolerance. Therefore, the characterization of genes and regulatory regions that might be useful for genetic transformation is indispensable. The isolation and characterization of new regulatory regions is of great importance to drive transgene expression in genetically modified crops. One of the major drawbacks in cotton production is pest damage; therefore, the most promising, cost-effective, and sustainable method for pest control is the development of genetically resistant cotton lines. Considering this scenario, our group isolated and characterized the promoter region of a MCO (multicopper oxidase) from Gossypium hirsutum, named GhAO-like1 (ascorbate oxidase-like1). The quantitative expression, together with the in vivo characterization of the promoter region reveals that GhAO-like1 has a flower- and fruit-specific expression pattern. The GUS activity is mainly observed in stamens, as expected considering that the GhAO-like1 regulatory sequence is enriched in cis elements, which have been characterized as a target of reproductive tissue specific transcription factors. Both histological and quantitative analyses in Arabidopsis thaliana have confirmed flower (mainly in stamens) and fruit expression of GhAO-like1. In the present paper, we isolated and characterized both in silico and in vivo the promoter region of the GhAO-like1 gene. The regulatory region of GhAO-like1 might be useful to confer tissue-specific expression in genetically modified plants. PMID:26692462

  17. Bioavailability of the sodium pertechnetate and morphometry of organs isolated from rats: study of possible pharmacokinetic interactions of a ginkgo biloba extract

    Energy Technology Data Exchange (ETDEWEB)

    Moreno, Silvana Ramos Farias; Arnobio, Adriano; Caldas, Luiz Querino de Araujo [Universidade Federal Fluminense, Niteroi, RJ (Brazil). Programa de Pos-graduacao em Ciencias Medicas]. E-mail: srfmoreno@hotmail.com; Carvalho, Jorge Jose; Nascimento, Ana Lucia; Pereira, Mario [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Dept. de Histologia e Embriologia; Dire, Glaucio; Bernardo Filho, Mario [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Dept. de Biofisica e Biometria; Rocha, Emely Kazan [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Dept. de Biologia Celular e Genetica

    2005-10-15

    Many compounds affect the bioavailability of radiobiocomplexes as radiopharmaceuticals. Ginkgo Biloba extract (EGb) has several effects. The influence of an EGb on the bioavailability of the radiobiocomplex sodium pertechnetate (Na{sup 99m} TcO{sub 4}) and on the morphometry of the organs was evaluated. Rats were treated with EGb and Na{sup 99m} TcO{sub 4} was injected. The animals were sacrificed; the radioactivity in the organs was counted. The results showed that EGb altered the Na{sup 99m} TcO{sub 4} bioavailability in the kidneys, liver and duodenum. Morphometric analysis of the organs showed significant alterations (P<0.05), probably caused by metabolites generated by EGb and capable of altering the bioavailability of the Na{sup 99m} TcO{sub 4}. (author)

  18. Pythium kandovanense sp. nov., a fungus-like eukaryotic micro-organism (Stramenopila, Pythiales) isolated from snow-covered ryegrass leaves.

    Science.gov (United States)

    Chenari Bouket, Ali; Arzanlou, Mahdi; Tojo, Motoaki; Babai-Ahari, Asadollah

    2015-08-01

    Pythiumkandovanense sp. nov. (ex-type culture CCTU 1813T = OPU 1626T = CBS 139567T) is a novel oomycete species isolated from Lolium perenne with snow rot symptoms in a natural grassland in East-Azarbaijan province, Iran. Phylogenetic analyses based on sequence data from internal transcribed spacer (ITS)-rDNA, coxI and coxII mitochondrial genes clustered our isolates in Pythium group E as a unique, well supported clade. Pythium kandovanense sp. nov. is phylogenetically and morphologically distinct from the other closely related species in this clade, namely Pythium rostratifingens and Pythium rostratum. Pythium kandovanense sp. nov. can be distinguished from these two species by its cylindrical sporangia and lower temperatures for optimum and maximum growth rate. The development of zoospores released through a shorter discharge tube is an additional morphological feature which can be used to differentiate Pythium kandovanense sp. nov. from Pythium rostratifingens. Laboratory inoculation tests demonstrated the pathogenicity of Pythium kandovanense sp. nov. to L. perenne under wet cold (0-3 °C) conditions. PMID:25933619

  19. An in vivo/in vitro evaluation of Ginkgo Biloba extract on the biodistribution of sodium 99mTc- Pertechnetate and on the morphology of organs isolated from the rats

    International Nuclear Information System (INIS)

    The use of radionuclides for a multitude of basic research applications has continued to grow at a very rapid pace.Technetium-99m(99mTc) is the most widely used radionuclide in clinical nuclear medicine. Although serendipity played a significant role in its choice, 99mTc does possess both chemical and physical characteristics which make it the workhorse of radionuclide imaging and it has been used for labeling of various radiopharmaceuticals. Many substances have been reported to affect the biodistribution of different radiopharmaceuticals. Ginkgo Biloba extract (EGb) is a phytoterapic used in the treatment of hypoxic conditions. This medicinal plant has several biological effects, specially, vasodilator, anti-inflammatory and anti-coagulant properties. It has antioxidant characteristics.We evaluated the influence of an EGb on the biodistribution of the sodium 99mTc-pertechnetate (99mTcO4Na) and on the morphology of the organs from WISTAR rats. The animals were treated (6 days, intragastric via) with EGb (40 and 400 mg/mL). After that, 99mTcO4 Na was injected and the animals were sacrificed (after 10 minutes). The organs were isolated and counted in a well counter. The percentages of radioactivity per organ (%ATI/organ) and radioactivity per gram (%ATI/gram) of each organ were calculated. Histological preparations were carried out with the pieces of organs withdrawn from the treated animals (400mg/mL EGb). The results showed that EGb altered (not significant, n=5, p>0.05) the biodistribution of the 99mTcO4 Na in the kidneys and liver. A significant (n=5, P99mTcO4 Na in the treated animals

  20. Complete Genome Sequence of a Gluconacetobacter hansenii ATCC 23769 Isolate, AY201, Producer of Bacterial Cellulose and Important Model Organism for the Study of Cellulose Biosynthesis

    Science.gov (United States)

    Mehta, Kalpa

    2016-01-01

    The cellulose producer and model organism used for the study of cellulose biosynthesis, Gluconacetobacter hansenii AY201, is a variant of G. hansenii ATCC 23769. We report here the complete nucleotide sequence of G. hansenii AY201, information which may be utilized to further the research into understanding the genes necessary for cellulose biosynthesis. PMID:27516506

  1. Complete Genome Sequence of a Gluconacetobacter hansenii ATCC 23769 Isolate, AY201, Producer of Bacterial Cellulose and Important Model Organism for the Study of Cellulose Biosynthesis.

    Science.gov (United States)

    Pfeffer, Sarah; Mehta, Kalpa; Brown, R Malcolm

    2016-01-01

    The cellulose producer and model organism used for the study of cellulose biosynthesis, Gluconacetobacter hansenii AY201, is a variant of G. hansenii ATCC 23769. We report here the complete nucleotide sequence of G. hansenii AY201, information which may be utilized to further the research into understanding the genes necessary for cellulose biosynthesis. PMID:27516506

  2. Isolated Pelvic Hyperthermochemotherapeutic Perfusion -An Experimental Study on Isolating Efficacy

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Hyperthermochemotherapeutic perfusion model through isolated pelvic vessels was developed to evaluate the leakage of hyperthermia and drugs (such as adriamycin) from the isolated pelvic circulation to systemic circulation and its associated side/toxic effects. The isolated pelvic circulation was perfused through a femoral artery catheter with hyperthermic (48 ℃ to 55 ℃) adriamycin solution (50 μg/ml) for 30 min. The efflux was drained through a femoral vein catheter. And the pelvic temperature was kept at the level of 43±0.5 ℃. The temperature of pelvic circulation was kept at 4 ℃ to 5 ℃ greater than the systemic/core temperature. The adriamycin concentration of pelvic efflux was 12 to 46 folds of that of systemic serum. The difference between them was very significant (P<0.001). As the perfusion pressure was increased, which kept lower than the mean systemic artery pressure, the leakage of the adriamycin from the isolated pelvic circulation to systemic circulation was increased, but there was no significant difference between them (P>0.05). During isolated perfusion, the systemic blood dynamics remained stable and there were no organic injuries on the important organs. It was suggested that the isolating efficacy of the modality of isolated pelvic hyperthermochemotherapeutic perfusion through vessels was rather high. The hyperthermia and drugs could be effectively limited in the isolated pelvic region with minor side effects on the systemic circulation and important organs.

  3. SCREENING, ISOLATION, IDENTIFICATION OF HOMOFERMENTATIVE BACILLI AND HOMOFERMENTATIVE COCCI FROM IDLI BATTER AND ESTIMATION OF LACTIC ACID PRODUCED BY THEM TO GET THE HIGHEST YIELD GIVING ORGANISM

    OpenAIRE

    Pathak LP*, Mali RD and PS Deshmukh

    2013-01-01

    Lactic acid is an organic acid produced by bacterial fermentation. It has several industrial applications. Lactic acid has been produced on commercial scale using various Lactic acid bacteria.   Lactobacillus leishmanii is the most commonly employed species4. Screening for high yielding Lactic acid producing bacillus or coccus from natural source will improve its commercial value. Lactic acid bacteria include some rod shaped genera and some genera with the morphology, cocci2. They are abundan...

  4. Identification of detoxification pathways in plants that are regulated in response to treatment with organic compounds isolated from oil sands process-affected water.

    Science.gov (United States)

    Widdup, Ellen E; Chatfield-Reed, Kate; Henry, Darren; Chua, Gordon; Samuel, Marcus A; Muench, Douglas G

    2015-11-01

    Bitumen mining in the Athabasca oil sands region of northern Alberta results in the accumulation of large volumes of oil sands process-affected water (OSPW). The acid-extractable organic (AEO) fraction of OSPW contains a variety of compounds, including naphthenic acids, aromatics, and sulfur- and nitrogen-containing compounds that are toxic to aquatic and terrestrial organisms. We have studied the effect of AEO treatment on the transcriptome of root and shoot tissues in seedlings of the model plant, Arabidopsis thaliana. Several genes encoding enzymes involved in the xenobiotic detoxification pathway were upregulated, including cytochrome P450s (CYPs), UDP-dependent glycosyltransferases (UGTs), glutathione-S-transferases (GSTs), and membrane transporters. In addition, gene products involved in oxidative stress, β-oxidation, and glucosinolate degradation were also upregulated, indicating other potential mechanisms of the adaptive response to AEO exposure. These results provide insight into the pathways that plants use to detoxify the organic acid component of OSPW. Moreover, this study advances our understanding of genes that could be exploited to potentially develop phytoremediation and biosensing strategies for AEO contaminants resulting from oil sands mining. PMID:26052061

  5. Isolated dextrogastria

    Energy Technology Data Exchange (ETDEWEB)

    Cha, Kyung Soo; Kim, Soo Ryun; Lee, Yong Chul; Park, Soo Soung [Chung Ang University College of Medicine, Seoul (Korea, Republic of); Sim, Young Soo [Seoul National University College of Medicine, Seoul (Korea, Republic of)

    1982-06-15

    Isolated situs inversus of the stomach with otherwise normal position of the thoracic and abdominal viscera is an extremely rare anomaly occurring in two district forms. Majority of cases are associated with eventration of the diphragm and are reported as being confused with spontaneous pneumothorax or pyopneumothorax at base of the right lung. The right sided stomach may produce interesting and confusing changes in liver scan. We have experienced 2 cases of the isolated dextrogastria.

  6. Isolated dextrogastria

    International Nuclear Information System (INIS)

    Isolated situs inversus of the stomach with otherwise normal position of the thoracic and abdominal viscera is an extremely rare anomaly occurring in two district forms. Majority of cases are associated with eventration of the diphragm and are reported as being confused with spontaneous pneumothorax or pyopneumothorax at base of the right lung. The right sided stomach may produce interesting and confusing changes in liver scan. We have experienced 2 cases of the isolated dextrogastria

  7. Bioavailability of the sodium pertechnetate and morphometry of organs isolated from rats: study of possible pharmacokinetic interactions of a ginkgo biloba extract

    Directory of Open Access Journals (Sweden)

    Silvana Ramos Farias Moreno

    2005-10-01

    Full Text Available Many compounds affect the bioavailability of radiobiocomplexes as radiopharmaceuticals. Ginkgo Biloba extract (EGb has several effects. The influence of an EGb on the bioavailability of the radiobiocomplex sodium pertechnetate (Na99mTcO4 and on the morphometry of the organs was evaluated. Rats were treated with EGb and Na99mTcO4 was injected. The animals were sacrificed; the radioactivity in the organs was counted. The results showed that EGb altered the Na99mTcO4 bioavailability in the kidneys, liver and duodenum. Morphometric analysis of the organs showed significant alterations (PSubstâncias podem interferir na biodisponibilidade de radiobiocomplexos, como os radiofármacos. O extrato de Ginkgo Biloba (EGb apresenta efeitos. Avaliou-se a influência de um EGb na biodisponibilidade do pertecnetato de sódio (99mTcO-4Na e na morfometria de órgãos de ratos que foram tratados com EGb. 99mTcO-4Na foi injetado, os animais sacrificados e a radioatividade nos órgãos contada. Os resultados mostraram que o EGb alterou a biodisponibilidade do 99mTcO-4Na em rins, fígado e duodeno e alterações morfométricas significativas (p<0.05 foram encontradas. Sugere-se que o EGb poderia gerar metabólitos capazes de alterar morfometricamente os órgãos citados e alterar a biodisponibilidade do 99mTcO-4Na.

  8. Isolation and functional characterization of N-methyltransferases that catalyze betaine synthesis from glycine in a halotolerant photosynthetic organism Aphanothece halophytica.

    Science.gov (United States)

    Waditee, Rungaroon; Tanaka, Yoshito; Aoki, Kenji; Hibino, Takashi; Jikuya, Hiroshi; Takano, Jun; Takabe, Tetsuko; Takabe, Teruhiro

    2003-02-14

    Glycine betaine (N,N,N-trimethylglycine) is an important osmoprotectant and is synthesized in response to abiotic stresses. Although almost all known biosynthetic pathways of betaine are two-step oxidation of choline, here we isolated two N-methyltransferase genes from a halotolerant cyanobacterium Aphanothece halophytica. One of gene products (ORF1) catalyzed the methylation reactions of glycine and sarcosine with S-adenosylmethionine acting as the methyl donor. The other one (ORF2) specifically catalyzed the methylation of dimethylglycine to betaine. Both enzymes are active as monomers. Betaine, a final product, did not show the feed back inhibition for the methyltransferases even in the presence of 2 m. A reaction product, S-adenosyl homocysteine, inhibited the methylation reactions with relatively low affinities. The co-expressing of two enzymes in Escherichia coli increased the betaine level and enhanced the growth rates. Immunoblot analysis revealed that the accumulation levels of both enzymes in A. halophytica cells increased with increasing the salinity. These results indicate that A. halophytica cells synthesize betaine from glycine by a three-step methylation. The changes of amino acids Arg-169 to Lys or Glu in ORF1 and Pro-171 to Gln and/or Met-172 to Arg in ORF2 significantly decreased V(max) and increased K(m) for methyl acceptors (glycine, sarcosine, and dimethylglycine) but modestly affected K(m) for S-adenosylmethionine, indicating the importance of these amino acids for the binding of methyl acceptors. Physiological and functional properties of methyltransferases were discussed. PMID:12466265

  9. Marine organisms as source of extracts to disrupt bacterial communication: bioguided isolation and identification of quorum sensing inhibitors from Ircinia felix

    Directory of Open Access Journals (Sweden)

    Jairo Quintana

    2015-06-01

    Full Text Available AbstractIn this study, 39 extracts from marine organisms were evaluated as quorum sensing inhibitors, collected in the Colombian Caribbean Sea and the Brazilian Coast including 26 sponges, seven soft corals, five algae and one zooanthid. The results showed that crude extracts from the soft coral Eunicea laciniata, and the sponges Svenzea tubulosa, Ircinia felix and Neopetrosia carbonaria were the most promising source of quorum sensing inhibitors compounds without affecting bacterial growth, unlike the raw extracts of Agelas citrina, Agelas tubulata, Iotrochota arenosa, Topsentia ophiraphidites, Niphates caycedoi, Cliona tenuis, Ptilocaulis walpersi, Petrosia pellasarca, and the algae Laurencia catarinensis and Laurencia obtusa, which displayed potent antibacterial activity against the biosensors employed. The crude extract from the sponge I. felix was fractionated, obtaining furanosesterterpenes which were identified and evaluated as quorum sensing inhibitors, showing a moderate activity without affecting the biosensor's growth.

  10. Isolation of Diverse Structural Compartments of Natural Organic Matter from the Kolyma River Watershed in East Siberian Arctic Using DEAE-Cellulose, XAD-8 Resin, C18 and PPL Cartridges

    Science.gov (United States)

    Andzhushev, M.; Dubinenkov, I.; Holmes, R. M.; Hatfield, K.; Perminova, I.; Bulygina, E. B.; Konstantinov, A.

    2011-12-01

    Natural Organic Matter (NOM) is an essential part of the global carbon cycle and plays a significant role in transport of organic carbon from terrestrial ecosystems into the World Ocean. The Arctic region is one of the most vulnerable with respect to climate change. The Kolyma River is one of the great Arctic Rivers. The particular feature of the Kolyma River watershed is its location in the continuous permafrost zone. Hence, research on structural composition of NOM in the Kolyma River basin is very important for understanding the carbon flux and NOM transformations on the way from permafrost to the Arctic Ocean under conditions of the changing climate. The purpose of this work was to isolate diverse structural compartments of NOM from permafrost mud streams and freshwater environments of the Kolyma River basin suited for further structural studies using a suite of different sorbents. Another goal was to assess applicability of these sorbents for developing a NOM fluxmeter - passive device for in situ measurement of fluxes. The following sorbents were used in this study: diethylaminoethyl (DEAE) cellulose , XAD-8 resin, Varian Bond Elute PPL and C18-cartridges. The choice of the sorbents was based on the following considerations. DEAE-cellulose is an anion-exchanging resin. It is suited the best for isolation of negatively charged NOM constituents of high and low molecular weight which represent the major part of freshwater NOM. Given positive charge inherent within the sorbent, sorption of negatively charged compartments from natural water occurs under flow through conditions without any prior treatment. This makes the DEAE cellulose very promising for in situ applications (e.g., for fluxmeter). Amberlite XAD-8 is a macroreticular resin which is used as a part of the standard protocol of International Humic Substances Society for isolation of freshwater humic substances (HS). The XAD-8 resin represents a neutral hydrophobic polymer. As a result, for isolation of

  11. Isoler nu

    DEFF Research Database (Denmark)

    Andersen, N.E.; Ankerstjerne, P.; Jørgensen, B.;

    Omfattende vejledning, der angiver, hvordan energiforbruget i eksisterende huse kan nedbringes ved isolering, tætning og forbedring af varmeanlægget, og hvor meget det koster. Anvisningen indeholder detaljerede eksempler på efterisolering af ydervægge, tage, gulve og vinduer. Henvender sig til ej...

  12. Sugar Utilization Potential of Micropolyspora Isolated from Extreme Environment

    Directory of Open Access Journals (Sweden)

    A. B. Koli

    2012-01-01

    Full Text Available Problem statement: Waste water of various industries contain large amount of organic compounds which are continuously added in sea via river which causes water pollution. There is need to degrade the organic content to abate water pollution. Approach: To stabilize the organic content in sea water, there was need to search such an organism which was efficient to degrade organic content. We had screened the actinomycetes having potential to degrade organic compounds. Results: Total six isolates of Micropolyspora were obtained, from which all isolates were found to utilize galactose, 50% isolates were able to utilize arabinose, fructose and xylose. 83% of isolates showed mannitol utilization while 67% isolates uses lactose as carbon source and 33% isolates utilizes rhamnose. Conclusion/Recommendation: From the study performed, we conclude that, obtained most of Micropolyspora isolate may be used for the stabilization of organic content and hence in abatement of pollution.

  13. LEADERSHIP IN VIRTUAL ORGANIZATION

    OpenAIRE

    Marian NÃSTASE; Alexandru ROJA

    2013-01-01

    Current economic circumstances, very dynamic and characterized by uncertainty, require organizations to identify new sources of competitive advantage to remain competitive. Collaboration between organizations in collaborative networks are forms and environments in which organizations can use their distinctive potential. Capitalization of organizational potential in isolated manner, will not generate competitive advantage.Also important, the globalization and relocation of business, and integr...

  14. Eksistentiel Isolation

    OpenAIRE

    Matikainen, Oliver Albert; Andersen, Kian Alexander; Thorup, Johannes Hoff; Slotsager, Christian Knud; Jensen, Mette Ingersholm; Bachmann, Zenia Gruhl; Razga, Pauline Marie

    2015-01-01

    This study examines whether or not isolation can lead to personal growth. In order to answer this question we distinguish between loneliness and aloneness. We explain the theory of existential psychotherapist Irvin D. Yalom and on the basis of this examination, we analyze a conducted interview through the theory. Based on the theory, interview and analysis, we discuss the validity of Yalom’s theory. Considering the examination, the analysis and the distinction between the two types of isolati...

  15. Organ culture

    DEFF Research Database (Denmark)

    Alm, Rikard; Edvinsson, Lars; Malmsjö, Malin

    2002-01-01

    BACKGROUND: Endothelium dysfunction is believed to play a role in the development of cardiovascular disease. The aim of the present study was to evaluate the suitability of organ culture as a model for endothelium dysfunction. METHODS: The isometric tension was recorded in isolated segments of the...... rat mesenteric artery branch, before and after organ culture for 20 h. Vasodilatation was expressed as % of preconstriction with U46619. The acetylcholine (ACh) induced nitric oxide (NO) mediated dilatation was studied in the presence of 10 microM indomethacin, 50 nM charybdotoxin and 1 microM apamin....... Endothelium-derived hyperpolarising factor (EDHF) was studied in the presence of 0.1 mM L-NOARG and indomethacin. Prostaglandins were studied in the presence of L-NOARG, charybdotoxin and apamin. RESULTS: The ACh-induced NO and prostaglandin-mediated dilatations decreased significantly during organ culture...

  16. Cultura cromogênica dum bacilo ácido-álcool resistente isolado de pus de lesão fechada de lepra humana Chromogenic culture of an acid-fast organism isolated from human leprosy

    Directory of Open Access Journals (Sweden)

    H. C. de Souza-Araujo

    1942-01-01

    Full Text Available Last October 2nd the Author smeared nine tubes of Loewenstein medium with material obtained from closed pustulae of a seven years old boy, L2 case of leprosy. This material was very rich in Hansen bacilli in its different forms, inclusive globus, as is seen in the figures 2 and 3 of Plate 1. Part of this material obtained from pustulae opened by galvanocautery, was inoculated, at the same day, into white rats and guinea-pigs. November 26th a new biopsy gave more rich material, which was smeared again into Loewenstein fresh medium. December 15th three of the first and two of the second series of tubes of cultures showed germination of a yellow, dry and rough culture, covering almost the total surface of the medium. Microscopic examination of the culture showed that it was a pure culture of an acid-fast organism. Passages into glycerinated potatoes germinated well covering the surface of the same with a clear yellow granulated culture remaining the fluid (glycerinated water quite limpid. The germination in glycerinated broth produced a yellow velum in the surface of the medium, as is seen in fig. 3 of Plate 2, without becoming turbid. The microorganism isolated twice from the same source of material was cocciforme (as Mycobacterium pulviforme of Marchoux, in the original culture, becoming more bacilliforme, always acid-fast, after passage into glycerinated media. The A. sent his culture to foreigner colleagues to study it and will inoculate it soon into laboratory animals.

  17. Propriedades redox e grupos funcionais de ácidos húmicos isolados de adubos orgânicos Redox properties and functional groups of humic acids isolated from organic residues

    Directory of Open Access Journals (Sweden)

    Marihus Altoé Baldotto

    2007-06-01

    Full Text Available Dependendo das condições do sistema, os ácidos húmicos podem atuar como oxidantes ou redutores. Nos sistemas naturais, o fluxo de elétrons está diretamente associado à quantidade e à qualidade do húmus. O potencial do eletrodo e a capacidade de oxidação informam sobre os fatores intensidade e capacidade dos sistemas redox, respectivamente. Estudos prévios têm aventado a hipótese de que radicais livres nos ácidos húmicos participam dessas reações redox. No presente estudo, seis ácidos húmicos isolados de adubos orgânicos foram titulados com um oxidante (I2 em atmosfera inerte e condições especificadas. Os ácidos húmicos apresentaram valores do potencial formal-padrão do eletrodo semelhantes: entre 0,773 e 0,794 V a 25 ºC. A capacidade de oxidação dos ácidos húmicos variou de 3,88 a 4,39 mol c kg-1 a pH 5,0 e de 5,35 a 7,89 mol c kg-1 a pH 7,0. Foi observada correlação positiva e significativa entre a capacidade de oxidação dos ácidos húmicos e as suas concentrações de grupos funcionais fenólicos, quinonas e semiquinonas.According to the system conditions, humic acids act as oxidants or as reductants. In natural systems the electron flow is directly associated with humus content and quality. Electrode potential and oxidation capacity inform about the intensity and capacity of the redox systems. In previous studies it has been assumed that free radicals participate in humic acid redox reactions. In our study six humic acids isolated from composed organic residues were titrated with an oxidant (I2 in inert atmosphere and specified conditions. Humic acids presented similar standard formal electrode potentials, with values between 0.773 and 0.794 V, at 25 ºC. The oxidation capacity of humic acids varied from 3.88 to 4.39 mol c kg-1 at pH 5.0 and from 5.35 to 7.89 mol c kg-1 at pH 7.0. Positive and significant correlations were observed between the oxidation capacity and the concentrations of the phenolic

  18. Fasciola hepatica: Histology of the Reproductive Organs and Differential Effects of Triclabendazole on Drug-Sensitive and Drug-Resistant Fluke Isolates and on Flukes from Selected Field Cases

    Directory of Open Access Journals (Sweden)

    Robert Hanna

    2015-06-01

    Full Text Available This review summarises the findings of a series of studies in which the histological changes, induced in the reproductive system of Fasciola hepatica following treatment of the ovine host with the anthelmintic triclabendazole (TCBZ, were examined. A detailed description of the normal macroscopic arrangement and histological features of the testes, ovary, vitelline tissue, Mehlis’ gland and uterus is provided to aid recognition of the drug-induced lesions, and to provide a basic model to inform similar toxicological studies on F. hepatica in the future. The production of spermatozoa and egg components represents the main energy consuming activity of the adult fluke. Thus the reproductive organs, with their high turnover of cells and secretory products, are uniquely sensitive to metabolic inhibition and sub-cellular disorganisation induced by extraneous toxic compounds. The flukes chosen for study were derived from TCBZ-sensitive (TCBZ-S and TCBZ-resistant (TCBZ-R isolates, the status of which had previously been proven in controlled clinical trials. For comparison, flukes collected from flocks where TCBZ resistance had been diagnosed by coprological methods, and from a dairy farm with no history of TCBZ use, were also examined. The macroscopic arrangement of the reproductive system in flukes was studied using catechol/carmine stained whole mounts, and the histology of the main organs was examined using conventional haematoxylin-eosin stained sections. Validation of apoptosis in the fluke sections was carried out using an in situ hybridisation method designed to label endonuclease-induced DNA strand breaks. In TCBZ-S flukes exposed to TCBZ metabolites for 24–96 h in vivo, but not in TCBZ-R flukes, those tissues where active meiosis and/or mitosis occurred (testis, ovary, and vitelline follicles, were found to display progressive loss of cell content. This was due to apparent failure of cell division to keep pace with expulsion of the mature or

  19. Alternativ isolering i Tyskland

    DEFF Research Database (Denmark)

    Hansen, Ernst Jan de Place

    2001-01-01

    Resume af rapport om brugen af alternativ isolering i Tyskland, som er udarbejdet af Teknologisk Insititut under Energistyrelsens udviklingsprogram "Miljø- og arbejdsmiljøvenlig isolering"......Resume af rapport om brugen af alternativ isolering i Tyskland, som er udarbejdet af Teknologisk Insititut under Energistyrelsens udviklingsprogram "Miljø- og arbejdsmiljøvenlig isolering"...

  20. Organizations as Designed Islands

    Directory of Open Access Journals (Sweden)

    Pasquale Gagliardi

    2009-05-01

    Full Text Available The literature and practice of organizational design are mostly based on simplistic conceptions which ignore recent theoretical developments in organizational studies. Conceiving of organizations as ‘designed islands’, it is argued, can contribute to a more solid theoretical foundation to organization theory, viewed as normative science. Relying on the work of Peter Sloterdijk, who describes the forms of life in space in terms of spheres, the heuristic power of the island metaphor is explored. What can be learnt from the art of isolating in order to construct lived organizational environments is then discussed, and the paradoxical relationship between connection and isolation is highlighted.

  1. Genetic Diversity of Gallibacterium anatis Isolates from Different Chicken Flocks

    OpenAIRE

    Bojesen, Anders Miki; Torpdahl, Mia; Christensen, Henrik; Olsen, John Elmerdahl; Bisgaard, Magne

    2003-01-01

    Amplified fragment length polymorphisms (AFLPs) were used to characterize the genotypic diversity of a total of 114 Gallibacterium anatis isolates originating from a reference collection representing 15 biovars from four countries and isolates obtained from tracheal and cloacal swab samples of chickens from an organic, egg-producing flock and a layer parent flock. A subset of strains was also characterized by pulsed-field gel electrophoresis and biotyping. The organic flock isolates were char...

  2. Isolation and identification of local Bacillus isolates for xylanase biosynthesis.

    Directory of Open Access Journals (Sweden)

    Hassan Ammoneh

    2014-04-01

    Full Text Available Bacillus species are attractive industrial organisms due to their rapid growth rates leading to a short fermentation cycle and for their capacity to secrete important enzymes and proteins such as xylanase into the extracellular medium. Considering the industrial importance of xylanase, in this current study, Bacillus spp. were isolated from different soils and were screened for their xylanase production.Bacillus isolates used in this study were obtained from a national screening program carried out during 2006-2007 in which soil samples that covered areas throughout the interior of Syria were collected. The prepared inoculum from each of Bacillus isolates was aliquoted onto xylan agar plates, incubated at 30°C for 72 h and screened for xylanase synthesis.Xylanolytic isolates were selected depending on the clear zones of xylan hydrolysis. Fifteen isolates having the highest clearing zone were determined and grown in a solid state fermentation. Of the 15 isolates, three bacilli namely SY30A, SY185C and SY190E that showed maximum xylanase production, were identified using the 16S rDNA sequencing method. According to 16S rDNA gene sequence data, the closest phylogenetic neighbor for SY30A was Bacillus pumilus and for SY185C and SY190E isolates was Bacillus subtilis. Optimal pH and temperature for xylanase activity was 7.0 and 55ºC for SY30A and 6.0 and 60ºC for SY185C and SY190E, respectively. Under these conditions, the following activities were found to be around 1157 ± 58, 916 ± 46 and 794 ± 39 (U/g for SY30A, SY185C and SY190E, respectivly.Selected local Bacillus isolates were found to be a potential source of xylanase which was proven to be quite suitable for multiple biotechnological applications. These isolates might after extensive optimization steps be an alternative to commercially available strains.

  3. Organization within Organization Studies

    DEFF Research Database (Denmark)

    Lopdrup-Hjorth, Thomas

    This paper explores how prevalent contemporary problematizations of organizations coincide with a widespread assessment that Organization Studies (OS) has run out of steam. This impasse, the paper argues, is largely due to the emergence of an organization-phobia that has come to seize several...... strands of theorizing. By attending to the wide-ranging and far-reaching history of this organization-phobia, the paper argues that OS has become increasingly incapable of speaking about its core object. I show how organizations went from being conceptualized as entities of major importance to becoming...... associated with all kinds of ills. Through this history, organizations as distinct entities have been rendered so problematic that they have gradually come to be removed from the center of OS. The costs of this have been rather significant. Besides undermining the grounds that gave OS intellectual...

  4. Isolation of Saprophytic Basidiomycetes from Soil

    OpenAIRE

    Thorn, R.G.; Reddy, C. A.; Harris, D.; Paul, E. A.

    1996-01-01

    A method with the combined advantages of soil particle washing, selective inhibitors, and an indicator substrate was developed to isolate saprophytic basidiomycetes from soil. Organic particles were washed from soil and plated on a medium containing lignin, guaiacol, and benomyl, which reduced mold growth and allowed detection of basidiomycetes producing laccase or peroxidase. The 64 soil samples yielded 67 basidiomycete isolates, representing 51 groups on the basis of morphology and physiolo...

  5. Combating isolation: Building mutual mentoring networks

    Science.gov (United States)

    Cox, Anne J.

    2015-12-01

    Women physicists can often feel isolated at work. Support from a grant through the ADVANCE program of the National Science Foundation (U.S. government funding) created mutual mentoring networks aimed at combating isolation specifically for women faculty at undergraduate-only institutions. This paper will discuss the organization of one such network, what contributed to its success, some of the outcomes, and how it might be implemented in other contexts.

  6. Knowledge Organization = Information Organization?

    DEFF Research Database (Denmark)

    Hjørland, Birger

    Are the terms ―information organization‖ (IO), ―organization of information‖ (OI) and ―information architecture‖ (IA) synonyms for knowledge organization (KO)? This study uses bibliometric methods, among others, to determine some relations between these terms and their meanings. Apparently the data...

  7. STREPTOMYCETE Producing Antibiotics Isolated From Egyptian Soil

    International Nuclear Information System (INIS)

    An investigation was concerned the distribution of Streptomyces species in some types of Egyptian soils. Thirty nine actinomycetes isolates were obtained from 7 types of Egyptian soils; 8 isolates exhibited antimicrobial activities against all tested organisms and three of them were identified as Streptomyces griseoluteus (YM23), Streptomyces aurantiogriseus (S15) and Streptomyces nogalator (H12). RAPD-PCR showed correlation between 8 isolates in 3 clades. The active metabolite was extracted with ethyl acetate and concentrated in vacuum and the crude fraction was purified using thin layer and column chromatography

  8. Understand Quarantine and Isolation

    Science.gov (United States)

    ... Social Media What CDC is Doing Blog: Public Health Matters What's New Preparation & Planning Emergency Preparedness and Response Understand Quarantine and Isolation Recommend on Facebook Tweet Share Compartir Isolation and quarantine help protect the public by ...

  9. Genetic diversity of Gallibacterium anatis isolates from different chicken flocks

    DEFF Research Database (Denmark)

    Bojesen, A.M.; Torpdahl, Mia; Christensen, H.;

    2003-01-01

    chickens from an organic, egg-producing flock and a layer parent flock. A subset of strains was also characterized by pulsed-field gel electrophoresis and biotyping. The organic flock isolates were characterized by more than 94% genetic similarity, indicating that only a single clone was apparent in the...... flock. The layer parent flock isolates were grouped into two subclusters, each with similarity above 90%. One subcluster contained only tracheal isolates, while the other primarily included cloacal isolates. In conclusion, we show that AFLP analysis enables fingerprinting of G. anatis, which seems to...

  10. Epidemiology of extended spectrum β-lactamase, AmpC and class A carbapenemases-producing organisms isolated at San Camillo Hospital of Treviso (Italy between April 2012 and March 2014

    Directory of Open Access Journals (Sweden)

    Margherita Scapaticci

    2016-03-01

    Full Text Available The indiscriminate use of broad-spectrum cephalosporins of the last years has favoured the selection of extended spectrum β-lactamases (ESBLs, AmpC and class A carbapenemases (KPC-producing Enterobacteriaceae strains, representing a real health emergency. At San Camillo Hospital of Treviso, Italy, between April 2012 and March 2014, we isolated 263 suspected ESBL-producing strains from various specimens, including urine (76.4%, wound swabs (9.9%, blood cultures (4.6%, vaginal swabs (2.7%, fragments of bone (1.5% and other materials (4.9%. The majority of the isolated bacteria were represented by Escherichia coli (43.3%, followed by Klebsiella pneumoniae (34.2%, Proteus mirabilis (15.2%, Enterobacter spp. (3.8%, Morganella morganii (1.1%, Serratia spp. (0.8%, Proteus vulgaris (0.4%, Citrobacter freudii (0.4%, Providencia spp. (0.4% and Pseudomonas aeruginosa (0.4%. Using confirmatory phenotypic tests, 89.4% of the isolated resulted ESBL producer, 15.3% of which were also AmpC-producers, 1.5% were ESBL negative and AmpC positive, 4.2% were ESBL negative and AmpC negative, and 4.9%, consisting solely of K.pneumoniae, were confirmed as KPC positive. ESBL-mediated resistance to cephalosporin is not always clearly evident using susceptibility testing performed by agar diffusion-disc or dilution methods, for this reason it is strictly recommended to use specific tests able to reveal important mechanisms of resistance. The optimal use of diagnostic tools in microbiology is necessary to fight the spreading of pathogens with multiple antibiotic resistance mechanisms and in order to avoid giving useless antibiotic therapies to the patients.

  11. Earthquakes: Isolation, energy dissipation and control of vibrations of structures for nuclear and industrial facilities and buildings. Overview of lectures and papers of a seminar organized jointly with the Italian Working Group on Seismic Isolation (GLIS) and held in Capri, Italy, 23-25 August 1993

    International Nuclear Information System (INIS)

    This report summarizes the contributions to the seminar together with the main technical issues and conclusions. Particular attention is paid to contributions which provided new or updated information with respect to that given at the IAEA Specialists Meeting on Seismic Isolation Technology, held at San Jose (California, USA), 18-20 March 1992. Attention is also paid to the development and implementation of more recent but very promising innovative techniques for the reduction of seismic and other dynamic loads. 64 refs, 1 tab

  12. Epidemiology of extended spectrum β-lactamase, AmpC and class A carbapenemases-producing organisms isolated at San Camillo Hospital of Treviso (Italy) between April 2012 and March 2014

    OpenAIRE

    Margherita Scapaticci; Giacomo Fossen; Vittorina Ius

    2016-01-01

    The indiscriminate use of broad-spectrum cephalosporins of the last years has favoured the selection of extended spectrum β-lactamases (ESBLs), AmpC and class A carbapenemases (KPC)-producing Enterobacteriaceae strains, representing a real health emergency. At San Camillo Hospital of Treviso, Italy, between April 2012 and March 2014, we isolated 263 suspected ESBL-producing strains from various specimens, including urine (76.4%), wound swabs (9.9%), blood cultures (4.6%), vaginal swabs (2.7%)...

  13. Genetics Home Reference: isolated growth hormone deficiency

    Science.gov (United States)

    ... deficiency dwarfism, pituitary growth hormone deficiency dwarfism isolated GH deficiency isolated HGH deficiency isolated human growth hormone deficiency isolated somatotropin deficiency isolated somatotropin deficiency disorder ...

  14. Isolated microalbuminuria indicates a poor medical prognosis

    NARCIS (Netherlands)

    Scheven, Lieneke; Van der Velde, Marije; Heerspink, Hiddo J. Lambers; De Jong, Paul E.; Gansevoort, Ron T.

    2013-01-01

    Background. Microalbuminuria is often regarded as a sign of end-organ damage due to diabetes and/or hypertension, and as such to be associated with an increased risk for cardiovascular events. It has been questioned whether isolated microalbuminuria, that is microalbuminuria in the absence of a card

  15. Isolation of actinomycetes from cervical specimens.

    OpenAIRE

    Traynor, R M; Parratt, D; Duguid, H L; Duncan, I D

    1981-01-01

    Cervical smears from 30 women were examined. Fifteen of these had actinomycetes visible by direct smear examination and the organisms were isolated in 13. Of the remainder, all had negative smears and culture was similarly negative in 12, whilst in 3, the cultures were either positive (1) or suspicious (2).

  16. Isolation and cultivation of Lyme disease spirochetes.

    OpenAIRE

    Barbour, A G

    1984-01-01

    The successful isolation and cultivation of Lyme disease spirochetes traces its lineage to early attempts at cultivating relapsing fever borreliae. Observations on the growth of Lyme disease spirochetes under different in vitro conditions may yield important clues to both the metabolic characteristics of these newly discovered organisms and the pathogenesis of Lyme disease. Images FIG. 1

  17. Particulate Organic Matter (POM) Separation

    International Nuclear Information System (INIS)

    Information on soil organic matter (SOM) pools is of vital importance for studying the impact of soil management and environmental factors on soil organic carbon, an important part of the global carbon cycle. Several conceptual SOM pools with different turnover rates are available to feed models or to study carbon cycles. The fractionation scheme of Zimmermann allows isolating the labile particulate organic matter (POM) pool. Besides its use in conventional soil organic carbon dynamics studies and modelling, this pool can be determining as well in the evaluation of soil organic carbon stability based on the use of stable 15N and 13C isotopes

  18. Isolated Galaxies and Isolated Satellite Systems

    CERN Document Server

    Ann, H B; Choi, Yun-Young

    2009-01-01

    We search for isolated galaxies using a volume-limited sample of galaxies with 0.02r_{vir,nei} and \\rho <\\bar{\\rho} well segregates the CIG galaxies. We confirm the morphology conformity between the host and their satellites, which suggests importance of hydrodynamic interaction among galaxies within their virial radii in galaxy evolution.

  19. Isolation of Borrelia spirochetes from patients in Texas.

    OpenAIRE

    Rawlings, J A; Fournier, P V; Teltow, G. J.

    1987-01-01

    The Texas Department of Health Laboratory began culturing the Lyme disease spirochete Borrelia burgdorferi in 1985. This organism was subsequently isolated from blood, cerebrospinal fluid, joint fluid, skin, bone, and autopsy tissues from humans. Fluorescent-antibody tests with murine monoclonal antibodies confirmed that seven of these isolates were B. burgdorferi and that two others belonged to the genus Borrelia.

  20. DNA ISOLATION FROM SMALL TISSUE SAMPLES USING SALT AND SPERMINE

    Science.gov (United States)

    Common DNA isolation methods rely upon protein denaturation by organic solvents such as phenol and chloroform. hese solvents pose some risk to the user and require special disposal procedures. e have previously reported a method for isolating DNA from peripheral blood lymphocytes...

  1. Isolating the shaft

    International Nuclear Information System (INIS)

    Paper describes the environment contact preventing isolation of the shaft used to dispose off waste at the Dounreay site (Great Britain) and containing a mixture of radioactive and chemical waste. Well drilling, hydraulic testing, ability to work of isolation are phases of the working. Application of remote controlled transport facility for the waste removal from horizontal tunnel is discussed. Financial problems are treated

  2. Review on recent advances in the analysis of isolated organelles

    Energy Technology Data Exchange (ETDEWEB)

    Satori, Chad P. [Department of Chemistry, University of Minnesota, Minneapolis, MN 55455 (United States); Kostal, Vratislav [Department of Chemistry, University of Minnesota, Minneapolis, MN 55455 (United States); Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno 616 00 (Czech Republic); Arriaga, Edgar A., E-mail: arriaga@umn.edu [Department of Chemistry, University of Minnesota, Minneapolis, MN 55455 (United States)

    2012-11-13

    Highlights: Black-Right-Pointing-Pointer Advancements in organelle release. Black-Right-Pointing-Pointer New approaches to fractionate organelles. Black-Right-Pointing-Pointer Updates on new techniques to characterize isolated organelles. - Abstract: The analysis of isolated organelles is one of the pillars of modern bioanalytical chemistry. This review describes recent developments on the isolation and characterization of isolated organelles both from living organisms and cell cultures. Salient reports on methods to release organelles focused on reproducibility and yield, membrane isolation, and integrated devices for organelle release. New developments on organelle fractionation after their isolation were on the topics of centrifugation, immunocapture, free flow electrophoresis, flow field-flow fractionation, fluorescence activated organelle sorting, laser capture microdissection, and dielectrophoresis. New concepts on characterization of isolated organelles included atomic force microscopy, optical tweezers combined with Raman spectroscopy, organelle sensors, flow cytometry, capillary electrophoresis, and microfluidic devices.

  3. Isolation and screening phosphate solubilizers from composts as biofertilizer

    International Nuclear Information System (INIS)

    Phosphate solubilizers are miroorganisms that able to solubilize insoluble inorganic phosphate compounds or hydrolyze organic phosphate to inorganic P. Therefore make the P to be available for plant and consequently enhance plant growth and yield. Recently, phosphate solubilizing microorganisms has been shown to play an important role in the biofertilizer industry. Fifty-one bacterial were isolated from eleven composts. Most of the phosphate solubilizers were isolated from natural farming composted compost and normal composting compost. This shows that both of these composts are more suitable to use for phosphate solubilizer isolation compare commercial composts. Fourteen of the isolates were found to be phosphate solubilizers. These isolates produced a clear zone on the phosphate agar plates, showing their potential as biofertilizer. AP3 was significantly produced the largest clear zone compared with other isolates. This indicates that isolate AP 3 could be a good phosphate solubilizer. Thus, their effectiveness in the greenhouse and field should be evaluated. (Author)

  4. Isolated Asymptomatic Short Sternum in a Healthy Young Girl

    OpenAIRE

    Francesco Turturro; Cosma Calderaro; Antonello Montanaro; Luca Labianca; Giuseppe Argento; Andrea Ferretti

    2014-01-01

    Congenital sternal defects are rare deformities frequently associated with other anomalies of the chest wall and other organ systems. Although pectus excavatum, pectus carinatum, and cleft sternum can present as isolated deformity, in most cases they are associated with heart and inner organs anomalies and described as symptoms of syndromes like Marfan syndrome, Noonan syndrome, Poland anomaly, and Cantrell pentalogy. In contrast, the etiology of an isolated defect is not well understood. We ...

  5. Solid state 1H spin-lattice relaxation and isolated-molecule and cluster electronic structure calculations in organic molecular solids: The relationship between structure and methyl group and t-butyl group rotation

    Science.gov (United States)

    Wang, Xianlong; Mallory, Frank B.; Mallory, Clelia W.; Odhner, Hosanna R.; Beckmann, Peter A.

    2014-05-01

    We report ab initio density functional theory electronic structure calculations of rotational barriers for t-butyl groups and their constituent methyl groups both in the isolated molecules and in central molecules in clusters built from the X-ray structure in four t-butyl aromatic compounds. The X-ray structures have been reported previously. We also report and interpret the temperature dependence of the solid state 1H nuclear magnetic resonance spin-lattice relaxation rate at 8.50, 22.5, and 53.0 MHz in one of the four compounds. Such experiments for the other three have been reported previously. We compare the computed barriers for methyl group and t-butyl group rotation in a central target molecule in the cluster with the activation energies determined from fitting the 1H NMR spin-lattice relaxation data. We formulate a dynamical model for the superposition of t-butyl group rotation and the rotation of the t-butyl group's constituent methyl groups. The four compounds are 2,7-di-t-butylpyrene, 1,4-di-t-butylbenzene, 2,6-di-t-butylnaphthalene, and 3-t-butylchrysene. We comment on the unusual ground state orientation of the t-butyl groups in the crystal of the pyrene and we comment on the unusually high rotational barrier of these t-butyl groups.

  6. Solid state {sup 1}H spin-lattice relaxation and isolated-molecule and cluster electronic structure calculations in organic molecular solids: The relationship between structure and methyl group and t-butyl group rotation

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Xianlong, E-mail: WangXianlong@uestc.edu.cn, E-mail: pbeckman@brynmawr.edu [Key Laboratory for NeuroInformation of Ministry of Education, School of Life Science and Technology, University of Electronic Science and Technology of China, 4 North Jianshe Rd., 2nd Section, Chengdu 610054 (China); Mallory, Frank B. [Department of Chemistry, Bryn Mawr College, 101 North Merion Ave., Bryn Mawr, Pennsylvania 19010-2899 (United States); Mallory, Clelia W. [Department of Chemistry, Bryn Mawr College, 101 North Merion Ave., Bryn Mawr, Pennsylvania 19010-2899 (United States); Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6323 (United States); Odhner, Hosanna R.; Beckmann, Peter A., E-mail: WangXianlong@uestc.edu.cn, E-mail: pbeckman@brynmawr.edu [Department of Physics, Bryn Mawr College, 101 North Merion Ave., Bryn Mawr, Pennsylvania 19010-2899 (United States)

    2014-05-21

    We report ab initio density functional theory electronic structure calculations of rotational barriers for t-butyl groups and their constituent methyl groups both in the isolated molecules and in central molecules in clusters built from the X-ray structure in four t-butyl aromatic compounds. The X-ray structures have been reported previously. We also report and interpret the temperature dependence of the solid state {sup 1}H nuclear magnetic resonance spin-lattice relaxation rate at 8.50, 22.5, and 53.0 MHz in one of the four compounds. Such experiments for the other three have been reported previously. We compare the computed barriers for methyl group and t-butyl group rotation in a central target molecule in the cluster with the activation energies determined from fitting the {sup 1}H NMR spin-lattice relaxation data. We formulate a dynamical model for the superposition of t-butyl group rotation and the rotation of the t-butyl group's constituent methyl groups. The four compounds are 2,7-di-t-butylpyrene, 1,4-di-t-butylbenzene, 2,6-di-t-butylnaphthalene, and 3-t-butylchrysene. We comment on the unusual ground state orientation of the t-butyl groups in the crystal of the pyrene and we comment on the unusually high rotational barrier of these t-butyl groups.

  7. Organ Transplantation

    Science.gov (United States)

    ... their death. One strategy is simply to permit organ sale by changing the National Organ Transplant Act (NOTA), the federal law that bans organ sales. Then, individuals would be free to broker contracts ...

  8. Propagation and isolation of ranaviruses in cell culture

    DEFF Research Database (Denmark)

    Ariel, Ellen; Nicolajsen, Nicole; Christophersen, Maj-Britt;

    2009-01-01

    The optimal in vitro propagation procedure for a panel of ranavirus isolates and the best method for isolation of Epizootic haematopoietic necrosis virus (EHNV) from organ material in cell-culture were investigated. The panel of ranavirus isolates included: Frog virus 3 (FV3), Bohle iridovirus (BIV......), Pike-perch iridovirus (PPIV), European catfish virus (ECV), European sheatfish virus (ESV), EHNV, Doctor fish virus (DFV), Guppy virus 6 (GF6), short-finned eel virus (SERV) and Rana esculenta virus Italy 282/102 (REV 282/102). Each isolate was titrated in five cell lines: bluegill fry (BF-2...

  9. Base isolation: Fresh insight

    Energy Technology Data Exchange (ETDEWEB)

    Shustov, V.

    1993-07-15

    The objective of the research is a further development of the engineering concept of seismic isolation. Neglecting the transient stage of seismic loading results in a widespread misjudgement: The force of resistance associated with velocity is mostly conceived as a source of damping vibrations, though it is an active force at the same time, during an earthquake type excitation. For very pliant systems such as base isolated structures with relatively low bearing stiffness and with artificially added heavy damping mechanism, the so called `damping`` force may occur even the main pushing force at an earthquake. Thus, one of the two basic pillars of the common seismic isolation philosophy, namely, the doctrine of usefulness and necessity of a strong damping mechanism, is turning out to be a self-deception, sometimes even jeopardizing the safety of structures and discrediting the very idea of seismic isolation. There is a way out: breaking with damping dependancy.

  10. Isolated sleep paralysis

    OpenAIRE

    Sawant, Neena S.; Shubhangi R Parkar; Tambe, Ravindra

    2005-01-01

    Sleep paralysis (SP) is a cardinal symptom of narcolepsy. However, little is available in the literature about isolated sleep paralysis. This report discusses the case of a patient with isolated sleep paralysis who progressed from mild to severe SP over 8 years. He also restarted drinking alcohol to be able to fall asleep and allay his anxiety symptoms. The patient was taught relaxation techniques and he showed complete remission of the symptoms of SP on follow up after 8 months.

  11. Alternativ isolering i bygninger

    DEFF Research Database (Denmark)

    Hansen, Ernst Jan de Place

    2001-01-01

    Resume af rapport om målinger på alternative isoleringsmaterialer i bygninger, udgivet af Statens Byggeforskningsinstitut (SBI meddelelse 128) under Energistyrelsens udviklingsprogram "Miljø- og arbejdsmiljøvenlig isolering"......Resume af rapport om målinger på alternative isoleringsmaterialer i bygninger, udgivet af Statens Byggeforskningsinstitut (SBI meddelelse 128) under Energistyrelsens udviklingsprogram "Miljø- og arbejdsmiljøvenlig isolering"...

  12. Matching Organs

    Science.gov (United States)

    ... Donor Organs With Transplant Candidates When a deceased organ donor is identified, a transplant coordinator from an organ ... transplant candidate, you are registered on the national organ transplant waiting list. A living donor may also be identified and evaluated for living ...

  13. Terrestrial plants: a potent source for isolation of eco-friendly antifouling compounds

    Digital Repository Service at National Institute of Oceanography (India)

    Sawant, S.S.; Wagh, A.B.

    All over the world efforts are oriented towardes isolation of eco-friently antifouling toxins from marine plants and organisms. Consequently number of compounds having antifouling properties have been identified from marine plants and organisms...

  14. Isolation of Pasteurella multocida subspec. Multocida from chronic periapical lesion: First isolation in ex-Yugoslavia

    Directory of Open Access Journals (Sweden)

    Suvajdžić Ljiljana Ð.

    2006-01-01

    Full Text Available This study presents five isolates of Pasteurella multocida subsp. multo-cida isolated from chronic periapical inflammatory lesion. We described the methods of sampling and cultivation as well as diagnostic criteria. Pasteurella multocida was diagnosed on the basis of characteristic cultural and tinctorial properties and the facts that all strains produced indole and induced ornithine decarboxilation, glucose, saccharose and manitole fermentation. Isolates produced neither urease, nor fermented lactose and maltose. Further classification to subspecies multocida was based on the fact that all investigated isolates fermented trechalose, xylose and sorbitol the traits which are diagnostically significant for the species. Patients deny any contact with farm animals or pets, which indicates a possible aerosol transport and animal-human as well as human-human infection. We consider that this organism should be paid more attention by dentist, oral surgeons and microbiologists.

  15. Protolichesterinic acid, isolated from the lichen Cetraria islandica, reduces LRRC8A expression and volume-sensitive release of organic osmolytes in human lung epithelial cancer cells

    DEFF Research Database (Denmark)

    Thorsteinsdottir, Unnur Arna; Thorsteinsdottir, Margret; Lambert, Ian Henry

    2016-01-01

    cancer cells (A549) to PA (20 μg/mL, 24 h) reduces LRRC8A protein expression by 25% and taurine release following osmotic cell swelling (320 → 200 mOsm) by 60%. C75 (20 μg/mL, 24 h), a γ-lactone with a C8 carbon fatty acid chain, reduces VSOAC activity by 30%, i.e. less than PA. Stearic acid (20 μg......We have tested the effect of protolichesterinic acid (PA) on the activity of the volume-sensitive release pathway for the organic osmolyte taurine (VSOAC) and the expression of the leucine-rich-repeat-channel 8A (LRRC8A) protein, which constitutes an essential VSOAC component. Exposing human lung....../mL, 24 h) has no effect on VSOAC. Hence, length of PA's fatty acid chain adds to γ-lactone's inhibitory action. 5-Lipoxygenase (5-LO) activity is essential for swelling-induced activation of VSOAC. PA has no effect on cellular concentration of leukotrienes (5-HETE/LTB4) under hypotonic conditions...

  16. Magnetism in metal-organic capsules

    Energy Technology Data Exchange (ETDEWEB)

    Atwood, Jerry L.; Brechin, Euan K; Dalgarno, Scott J.; Inglis, Ross; Jones, Leigh F.; Mossine, Andrew; Paterson, Martin J.; Power, Nicholas P.; Teat, Simon J.

    2010-01-07

    Nickel and cobalt seamed metal-organic capsules have been isolated and studied using structural, magnetic and computational approaches. Antiferromagnetic exchange in the Ni capsule results from coordination environments enforced by the capsule framework.

  17. Isolation of technogenic magnetic particles

    Energy Technology Data Exchange (ETDEWEB)

    Catinon, Mickaël, E-mail: mickael.catinon@gmail.com [Laboratoire LECA, UMR 5553, Equipe Pollution, Environnement, Ecotoxicologie et Ecoremédiation, Univ. J. Fourier, 38041 Grenoble (France); Ayrault, Sophie, E-mail: sophie.ayrault@lsce.ispl.fr [Laboratoire des Sciences du Climat et de l' Environnement, UMR 8212, CEA-CNRS-UVSQ/IPSL, 91198 Gif-sur-Yvette (France); Boudouma, Omar, E-mail: boudouma@ccr.jussieu.fr [Service du MEB, UFR928, Université Pierre et Marie Curie, 75252 Paris VI (France); Bordier, Louise, E-mail: Louise.Bordier@lsce.ipsl.fr [Laboratoire des Sciences du Climat et de l' Environnement, UMR 8212, CEA-CNRS-UVSQ/IPSL, 91198 Gif-sur-Yvette (France); Agnello, Gregory, E-mail: contact@evinrude.fr [Evinrude, Espace St Germain, 38200 Vienne (France); Reynaud, Stéphane, E-mail: stephane.reynaud@ujf-grenoble.fr [Laboratoire LECA, UMR 5553, Equipe Pollution, Environnement, Ecotoxicologie et Ecoremédiation, Univ. J. Fourier, 38041 Grenoble (France); Tissut, Michel, E-mail: michel.tissut@ujf-grenoble.fr [Laboratoire LECA, UMR 5553, Equipe Pollution, Environnement, Ecotoxicologie et Ecoremédiation, Univ. J. Fourier, 38041 Grenoble (France)

    2014-03-01

    Technogenic magnetic particles (TMPs) emitted by various industrial sources, such as smelting plants, end up after atmospheric transfer on the soil surface. In the present study, we characterised the origin and composition of such particles emitted by a large iron smelting plant and deposited on particular substrates, namely tombstones, which act as a very interesting and appropriate matrix when compared to soil, tree bark, lichens or attic dust. The isolation and subsequent description of TMPs require a critical step of separation between different components of the sample and the magnetic particles; here, we described an efficient protocol that fulfils such a requirement: it resorts to water suspension, sonication, repeated magnetic extraction, sedimentation, sieving and organic matter destruction at 550 °C in some instances. The isolated TMPs displayed a noticeable crystalline shape with variable compositions: a) pure iron oxides, b) iron + Cr, Ni or Zn, and c) a complex structure containing Ca, Si, Mg, and Mn. Using Scanning Electron Microscope Energy Dispersive X-ray (SEM–EDX), we obtained profiles of various and distinct magnetic particles, which allowed us to identify the source of the TMPs. - Highlights: • The developed method offers a low-cost approach of large-scale dry deposition. • Tombstones are excellent supports for sampling these atmospheric deposits. • Smelted elements crystallise after cooling, giving typical technogenic magnetic particles (TMPs). • Coupling microscopic and bulk analyses allows identifying TMP origin. • Magnetic TMPs issued from steel industry were separated by a new technique.

  18. Isolation of technogenic magnetic particles

    International Nuclear Information System (INIS)

    Technogenic magnetic particles (TMPs) emitted by various industrial sources, such as smelting plants, end up after atmospheric transfer on the soil surface. In the present study, we characterised the origin and composition of such particles emitted by a large iron smelting plant and deposited on particular substrates, namely tombstones, which act as a very interesting and appropriate matrix when compared to soil, tree bark, lichens or attic dust. The isolation and subsequent description of TMPs require a critical step of separation between different components of the sample and the magnetic particles; here, we described an efficient protocol that fulfils such a requirement: it resorts to water suspension, sonication, repeated magnetic extraction, sedimentation, sieving and organic matter destruction at 550 °C in some instances. The isolated TMPs displayed a noticeable crystalline shape with variable compositions: a) pure iron oxides, b) iron + Cr, Ni or Zn, and c) a complex structure containing Ca, Si, Mg, and Mn. Using Scanning Electron Microscope Energy Dispersive X-ray (SEM–EDX), we obtained profiles of various and distinct magnetic particles, which allowed us to identify the source of the TMPs. - Highlights: • The developed method offers a low-cost approach of large-scale dry deposition. • Tombstones are excellent supports for sampling these atmospheric deposits. • Smelted elements crystallise after cooling, giving typical technogenic magnetic particles (TMPs). • Coupling microscopic and bulk analyses allows identifying TMP origin. • Magnetic TMPs issued from steel industry were separated by a new technique

  19. Genetic diversity of Gallibacterium anatis isolates from different chicken flocks

    DEFF Research Database (Denmark)

    Bojesen, A.M.; Torpdahl, Mia; Christensen, H.; Olsen, J.E.; Bisgaard, M.

    2003-01-01

    chickens from an organic, egg-producing flock and a layer parent flock. A subset of strains was also characterized by pulsed-field gel electrophoresis and biotyping. The organic flock isolates were characterized by more than 94% genetic similarity, indicating that only a single clone was apparent in the...

  20. Dynamics of Aeromonas species isolated from wastewater treatment system.

    Science.gov (United States)

    Martone-Rocha, S; Piveli, R P; Matté, G R; Dória, M C; Dropa, M; Morita, M; Peternella, F A; Matté, M H

    2010-12-01

    Aeromonas are widely distributed in the aquatic environment, and are considered to be emerging organisms that can produce a series of virulence factors. The present study was carried out in a sanitary sewage stabilization pond treatment system, located in Lins, State of São Paulo, Brazil. Most probable number was applied for estimation of the genus Aeromonas. Colony isolation was carried out on blood agar ampicillin and confirmed by biochemical characterization. Aeromonas species were isolated in 72.4% of influent samples, and in 55.2 and 48.3% of effluent from anaerobic and facultative lagoons, respectively. Thirteen Aeromonas species were isolated, representing most of the recognized species of these organisms. Even though it was possible to observe a tendency of decrease, total elimination of these organisms from the studied system was not achieved. Understanding of the pathogenic organism's dynamics in wastewater treatment systems with a reuse potential is especially important because of the risk it represents. PMID:20705981

  1. Seasonality of Legionella isolates from environmental sources.

    Science.gov (United States)

    Tobiansky, L; Drath, A; Dubery, B; Koornhof, H J

    1986-09-01

    Legionnaires' Disease in South Africa has tended to show a seasonal pattern of occurrence with peaks in late summer and autumn (November to May). We investigated the possibility of a correlation between the seasonal finding of the organism in environmental habitats and the occurrence of clinical cases. Results of tests performed on environmental specimens indicate that the isolation rate is higher in summer-autumn than during winter-spring. PMID:3793446

  2. Isolation of total RNA from pollens.

    Science.gov (United States)

    Bijli, K M; Singh, B P; Sridhara, S; Arora, N

    2001-05-01

    Isolation of total RNA from plant materials has been difficult, due to the presence of complex organic substances and the associated pigmentation. In fact, there is a dearth of standardized protocols for isolating total RNA from pollens. To find a simple and reliable method for isolating total RNA from pollen, four methods, viz. phenol/SDS (PS), guanidine HCl (GH), tri-reagent (TR), and modified SDS-betaME (SB) were tested with fresh pollen of Ricinus communis (procured at -70 degrees C) and pollen dried at 30-37 degrees C. The quality and quantity of RNA was superior for the material processed at -70 degrees C. SB gave the highest RNA yield (2.35 mg/g, OD260/280 >2.0), compared to other methods. The results obtained by the SB method were found to be comparable with the widely used tri-reagent method. This was validated with other pollens of Imperata cylindrica and Xanthium strumarium. The yield obtained from graded amounts of pollen was consistent with SB, compared to the TR method. The RNA isolated by SB gave good quality mRNA for synthesizing cDNA. The SDS-betaME method is simple, efficient, and uses less expensive reagents. Hence, we recommend the modified SDS-betaME method for isolating total RNA from pollens. PMID:11426703

  3. Permanent isolation surface barrier development plan

    International Nuclear Information System (INIS)

    The exhumation and treatment of wastes may not always be the preferred alternative in the remediation of a waste site. In-place disposal alternatives, under certain circumstances, may be the most desirable alternatives to use in the protection of human health and the environment. The implementation of an in-place disposal alternative will likely require some type of protective covering that will provide long-term isolation of the wastes from the accessible environment. Even if the wastes are exhumed and treated, a long-term barrier may still be needed to adequately dispose of the treated wastes or any remaining waste residuals. Currently, no open-quotes provenclose quotes long-term barrier is available. The Hanford Site Permanent Isolation Surface Barrier Development Program (BDP) was organized to develop the technology needed to provide a long-term surface barrier capability for the Hanford Site. The permanent isolation barrier technology also could be used at other sites. Permanent isolation barriers use engineered layers of natural materials to create an integrated structure with redundant protective features. Drawings of conceptual permanent isolation surface barriers are shown. The natural construction materials (e.g., fine soil, sand, gravel, riprap, asphalt) have been selected to optimize barrier performance and longevity. The objective of current designs is to use natural materials to develop a maintenance-free permanent isolation surface barrier that isolates wastes for a minimum of 1,000 years by limiting water drainage to near-zero amounts; reducing the likelihood of plant, animal, and human intrusion; controlling the exhalation of noxious gases; and minimizing erosion-related problems

  4. Isolation of Campylobacter from human stool samples

    Directory of Open Access Journals (Sweden)

    S M Salim

    2014-01-01

    Full Text Available Context: Campylobacter is an undetected cause of diarrhoea especially under 5 years of age in most of the countries. Isolation of this organism is difficult, expensive and cumbersome. Aims: Our objective of this study was to isolate this pathogen from the stool specimens on routinely available blood containing laboratory media using the candle jar for creating the microaerophilic atmosphere in our setup. Settings and Designs: A descriptive study. Materials and Methods: A total of 50 stool samples were inoculated onto selective and non-selective media with and without filtration using a 0.45 μm membrane. The inoculated media were simultaneously incubated in microaerophilic conditions using the Anoxomat as well as in candle jars at temperatures 37°C and 42°C. The culture isolates were confirmed by standard phenotypic tests. A simplex polymerase chain reaction (PCR targeting the 16S ribosomal deoxyribonucleic acid of Campylobacter was performed on the deoxyribonucleic acid (DNA of the culture isolates as well as on the DNA extracted from the stool filtrates. Statistical Analysis: Data was expressed as a proportion. Results: Campylobacter could be isolated in 5 out of 50 stool samples using both the Anoxomat as well as the candle jar. Furthermore, we did not find any difference between the isolation using the selective and blood containing media as well as the different incubation temperatures. All the five were confirmed phenotypically and genotypically to be Campylobacter jejuni. The PCR results corroborated with that of the culture. Conclusions: Isolation by culture was as sensitive as that of the PCR.

  5. Lever mechanism for vibration isolation

    Directory of Open Access Journals (Sweden)

    Mitchell Gohnert

    2010-04-01

    Full Text Available By introducing lever mechanism into the conventional vibration isolation system, new vibration isolation systems such as lever-damper isolator (LDI, lever-spring isolator (LSI and lever-spring-mass isolator (LSMI can be developed. The transmissibility of LDI, transmissibility of LSI and that of LSMI are obtained analytically. With numerical simulation, the vibration isolation performance of these systems and the effect of parameters on the performance are investigated. The results show that the performance of traditional vibration isolator can be improved by the introducing of lever mechanism. The results also show that the new systems have less constraint in design.

  6. Primary isolated hepatic tuberculosis

    International Nuclear Information System (INIS)

    Isolated hepatic tuberculosis without pulmonary or bowel involvement is a diagnostic challenge and can cause considerable morbidity. A young lady from Lahore presented with fever, pain in right hypochondria, nausea and weight loss. CT scan of abdomen showed multiple small hypodense non-enhancing lesions and a heterogeneous texture of liver. Biopsy confirmed the diagnosis of hepatic tuberculosis. It was concluded a case of isolated hepatic tuberculosis without evidence of other primary sites involvement. It is important to consider tuberculosis in the differential diagnosis when suspecting lymphoproliferative or metastatic diseases in a patient with vague symptoms and abnormal hepatic texture on CT. (author)

  7. Interaction of Treponema pallidum with isolated rabbit capillary tissues.

    OpenAIRE

    Quist, E E; Repesh, L A; Zeleznikar, R; Fitzgerald, T J

    1983-01-01

    Within infected tissue Treponema pallidum shows a characteristic predilection for perivascular areas. After intact capillaries had been prepared from rabbit brain tissue treponemes were incubated with isolated capillaries and visualised by darkfield, phase contrast, and scanning electron microscopy. The organisms rapidly attached to the surface of the capillaries at the tip of the treponeme; attached organisms retained motility for longer periods than unattached organisms. Treponema pertenue ...

  8. Local self-government development factors. Assessment of importance isolation and isolationism

    OpenAIRE

    Juri Plusnin

    2008-01-01

    The relationship between the development of local self-governance and their exclusion in modern Russia had found the research of municipal management organization. The hypothesis of local self-government development determination by factors of isolation and isolationism was proposed. The higher the isolation, the higher the level of local government and vice versa. Isolation promotes isolationism as a result of the factors limiting the development of local self-government. Types of isolation ...

  9. An Improved Method of RNA Isolation from Loblolly Pine (P. taeda L.) and Other Conifer Species

    OpenAIRE

    Lorenz, W Walter; Yu, Yuan-Sheng; Dean, Jeffrey F. D.

    2010-01-01

    Tissues isolated from conifer species, particularly those belonging to the Pinaceae family, such as loblolly pine (Pinus taeda L.), contain high concentrations of phenolic compounds and polysaccharides that interfere with RNA purification. Isolation of high-quality RNA from these species requires rigorous tissue collection procedures in the field and the employment of an RNA isolation protocol comprised of multiple organic extraction steps in order to isolate RNA of sufficient quality for mic...

  10. Proteoglycan isolation and analysis

    DEFF Research Database (Denmark)

    Woods, A; Couchman, J R

    Proteoglycans can be difficult molecules to isolate and analyze due to large mass, charge, and tendency to aggregate or form macromolecular complexes. This unit describes detailed methods for purification of matrix, cell surface, and cytoskeleton-linked proteoglycans. Methods for analysis of...

  11. Emotion regulation during isolation

    Czech Academy of Sciences Publication Activity Database

    Poláčková Šolcová, Iva; Šolcová, Iva

    2012-01-01

    Roč. 47, Suppl. 1 (2012). ISSN 0020-7594. [International Congress of Psychology /30./. 22.07.2012-27.07.2012, Cape Town] R&D Projects: GA ČR(CZ) GAP407/11/2226 Institutional support: RVO:68081740 Keywords : emotion regulation * isolation * Mars500 Subject RIV: AN - Psychology

  12. Isolated fructose malabsorption.

    OpenAIRE

    Wales, J. K.; Primhak, R A; Rattenbury, J; Taylor, C J

    1990-01-01

    A patient with isolated fructose malabsorption presented with diarrhoea and colic during the first year of life and subsequently responded to a fructose free diet. Fructose malabsorption has been implicated in some cases of irritable bowel syndrome in adults and may also be an infrequently recognised cause of gastrointestinal symptoms in children.

  13. Isolated mucosal Leishmaniasis

    OpenAIRE

    Deepak Sundriyal; Naveen Kumar; Raj Kumar; Brijesh Sharma

    2013-01-01

    Leishmaniasis is a term used to define a group of clinical syndrome caused by various species of parasite Leishmania. Three main clinical types of leishmaniasis are visceral leishmaniasis, cutaneous leishmaniasis and mucocutaneous leishmaniasis. However, isolated presentation as mucosal disease is rare. We report a case of primarily mucosal leishmaniasis.

  14. Chiral fiber optical isolator

    Science.gov (United States)

    Kopp, Victor I.; Zhang, Guoyin; Zhang, Sheng; Genack, Azriel Z.; Neugroschl, Dan

    2009-02-01

    We propose an in-fiber chiral optical isolator based on chiral fiber polarizer technology and calculate its performance by incorporating the magnetic field into the scattering matrix. The design will be implemented in a special preform, which is passed through a miniature heat zone as it is drawn and twisted. The birefringence of the fiber is controlled by adjusted the diameter of a dual-core optical fiber. By adjusting the twist, the fiber can convert linear to circular polarization and reject one component of circular polarization. In the novel central portion of the isolator, the fiber diameter is large. The effective birefringence of the circular central core with high Verdet constant embedded in an outer core of slightly smaller index of refraction is small. The central potion is a non-reciprocal polarization converter which passes forward traveling left circularly polarized (LCP) light as LCP, while converting backward propagating LCP to right circularly polarized (RCP) light. Both polarizations of light traveling backwards are scattered out of the isolator. Since it is an all-glass structure, we anticipate that the isolator will be able to handle several watts of power and will be environmentally robust.

  15. Isolating Triggered Star Formation

    Energy Technology Data Exchange (ETDEWEB)

    Barton, Elizabeth J.; Arnold, Jacob A.; /UC, Irvine; Zentner, Andrew R.; /KICP, Chicago /Chicago U., EFI; Bullock, James S.; /UC, Irvine; Wechsler, Risa H.; /KIPAC, Menlo

    2007-09-12

    Galaxy pairs provide a potentially powerful means of studying triggered star formation from galaxy interactions. We use a large cosmological N-body simulation coupled with a well-tested semi-analytic substructure model to demonstrate that the majority of galaxies in close pairs reside within cluster or group-size halos and therefore represent a biased population, poorly suited for direct comparison to 'field' galaxies. Thus, the frequent observation that some types of galaxies in pairs have redder colors than 'field' galaxies is primarily a selection effect. We use our simulations to devise a means to select galaxy pairs that are isolated in their dark matter halos with respect to other massive subhalos (N= 2 halos) and to select a control sample of isolated galaxies (N= 1 halos) for comparison. We then apply these selection criteria to a volume-limited subset of the 2dF Galaxy Redshift Survey with M{sub B,j} {le} -19 and obtain the first clean measure of the typical fraction of galaxies affected by triggered star formation and the average elevation in the star formation rate. We find that 24% (30.5 %) of these L* and sub-L* galaxies in isolated 50 (30) h{sup -1} kpc pairs exhibit star formation that is boosted by a factor of {approx}> 5 above their average past value, while only 10% of isolated galaxies in the control sample show this level of enhancement. Thus, 14% (20 %) of the galaxies in these close pairs show clear triggered star formation. Our orbit models suggest that 12% (16%) of 50 (30) h{sup -1} kpc close pairs that are isolated according to our definition have had a close ({le} 30 h{sup -1} kpc) pass within the last Gyr. Thus, the data are broadly consistent with a scenario in which most or all close passes of isolated pairs result in triggered star formation. The isolation criteria we develop provide a means to constrain star formation and feedback prescriptions in hydrodynamic simulations and a very general method of understanding

  16. Epidemiology and antimicrobial resistance of B. fragilis group organisms isolated from clinical specimen and human intestinal microbiota Epidemiologia e resistência a antimicrobianos de microorganismos do grupo B. fragilis isolados de espécime clínico e microbiota intestinal humana

    Directory of Open Access Journals (Sweden)

    Cibele Barreto Mano de Carvalho

    1996-10-01

    Full Text Available Epidemiological aspects and the antimicrobial susceptibility profile of the Bacteroides fragilis group isolated from clinical and human intestinal specimens were examined in this study. B. fragilis group strains were isolated from 46 (37% of 124 clinical specimens and the source of the samples was: Blood culture (3, intraabdominal infection (27, brain abscess (2, soft tissue infection (17, respiratory sinus (3, pleural aspirate (9, breast abscess (3, surgical infected wound (22, pelvic inflammatory disease (22, chronic otitis media (9 and miscellaneous (7. Intraabdominal and soft tissue infections were responsible for more than half of the clinical isolates. Susceptibility to penicillin, cefoxitin, tetracycline, metronidazole, chloramphenicol and clindamycin was examined. All isolates were susceptible to metronidazole and chloramphenicol. For clindamycin and cefoxitin the resistance rates observed were 21.7% and 10.9% respectively. Susceptibility profiles varied among the different species tested. A total of 37 species of B. fragilis group isolated from intestinal microbiota of individuals who had no antimicrobial therapy for at least 1 month before the sampling was also examined. All strains were also susceptible to chloramphenicol and motronidazole and the resistance rates to clindamycin and cefoxitin were 19.4% and 5.4% respectively. A few institutions, in Brazil, have monitored the antimicrobial susceptibility of B. fragilis group strains isolated from anaerobic infections. The resistance rates to cefoxitin and clindamycin and the variation in susceptibility patterns among the species isolated in this study emphasize the need for monitoring of susceptibility patterns of B. fragilis group organisms isolated, especially at our University Hospitals.Alguns aspectos epidemiológicos e o perfil de sensibilidade a antimicrobianos de amostras do grupo B. fragilis isoladas de espécime clínico e microbiota intestinal humana foram delineados neste

  17. Sugar Utilization Potential of Micropolyspora Isolated from Extreme Environment

    OpenAIRE

    A. B. Koli; Prakash Thorat; S. W. Kulkarni

    2012-01-01

    Problem statement: Waste water of various industries contain large amount of organic compounds which are continuously added in sea via river which causes water pollution. There is need to degrade the organic content to abate water pollution. Approach: To stabilize the organic content in sea water, there was need to search such an organism which was efficient to degrade organic content. We had screened the actinomycetes having potential to degrade organic compounds. Results: Total six isolates...

  18. High-Quality Draft Genome Sequence of Aneurinibacillus migulanus ATCC 9999T (DSM 2895), a Gramicidin S-Producing Bacterium Isolated from Garden Soil.

    Science.gov (United States)

    Wang, Jie-Ping; Liu, Bo; Liu, Guo-Hong; Ge, Ci-Bin; Xiao, Rong-Feng; Zheng, Xue-Fang; Shi, Huai

    2015-01-01

    Aneurinibacillus migulanus ATCC 9999(T) (DSM 2895) is a Gram-positive, round-spore-forming, and gramicidin S-producing bacterium. Here, we report the 6.35-Mb high-quality draft genome sequence of A. migulanus ATCC 9999(T), which will provide useful information for the genomic taxonomy and phylogenomics of Bacillus-like bacteria. PMID:26494674

  19. Method for isolating nucleic acids

    Science.gov (United States)

    Hurt, Jr., Richard Ashley; Elias, Dwayne A.

    2015-09-29

    The current disclosure provides methods and kits for isolating nucleic acid from an environmental sample. The current methods and compositions further provide methods for isolating nucleic acids by reducing adsorption of nucleic acids by charged ions and particles within an environmental sample. The methods of the current disclosure provide methods for isolating nucleic acids by releasing adsorbed nucleic acids from charged particles during the nucleic acid isolation process. The current disclosure facilitates the isolation of nucleic acids of sufficient quality and quantity to enable one of ordinary skill in the art to utilize or analyze the isolated nucleic acids for a wide variety of applications including, sequencing or species population analysis.

  20. Organic spintronics

    International Nuclear Information System (INIS)

    We review the emerging field of organic spintronics, where organic materials are applied as a medium to transport and control spin-polarized signals. The contacts for injecting and detecting spins are formed by ferromagnetic metals, oxides, or inorganic semiconductors. First, the basic concepts of spintronics and organic electronics are addressed, and phenomena which are in particular relevant for organic spintronics are highlighted. Experiments using different organic materials, including carbon nanotubes, organic thin films, self-assembled monolayers and single molecules are then reviewed. Observed magnetoresistance points toward successful spin injection and detection, but spurious magnetoresistance effects can easily be confused with spin accumulation. A few studies report long spin relaxation times and lengths, which forms a promising basis for further research. We conclude with discussing outstanding questions and problems. (topical review)

  1. Organic electroluminescence

    CERN Document Server

    Kafafi, Zakya H

    2005-01-01

    Organic light-emitting diode(OLED) technology has achieved significant penetration in the commercial market for small, low-voltage and inexpensive displays. Present and future novel technologies based on OLEDs involve rigid and flexible flat panel displays, solid-state lighting, and lasers. Display applications may range from hand-held devices to large flat panel screens that can be rolled up or hung flat on a wall or a ceiling. Organic Electroluminescence gives an overview of the on-going research in the field of organic light-emitting materials and devices, covering the principles of electroluminescence in organic thin films, as well as recent trends, current applications, and future potential uses. The book begins by giving a background of organic electroluminescence in terms of history and basic principles. It offers details on the mechanism(s) of electroluminescence in thin organic films. It presentsin-depth discussions of the parameters that control the external electroluminescence quantum efficien...

  2. Transnational Organizing

    DEFF Research Database (Denmark)

    Henriksen, Lasse Folke; Seabrooke, Leonard

    2015-01-01

    An ongoing question for institutional theory is how organizing occurs transnationally, where institution building occurs in a highly ambiguous environment. This article suggests that at the core of transnational organizing is competition and coordination within professional and organizational...... networks over who controls issues. Transnational issues are commonly organized through professional battles over how issues are treated and what tasks are involved. These professional struggles are often more important than what organization has a formal mandate over an issue. We highlight how ‘issue...... should be treated and governed by organizations. Using network and career sequences methods, we provide a case of transnational organizing through professionals who attempt issue control and network management on transnational environmental sustainability certification. The article questions how...

  3. Organic salmon

    DEFF Research Database (Denmark)

    Ankamah Yeboah, Isaac; Nielsen, Max; Nielsen, Rasmus

    The year 2016 is groundbreaking for organic aquaculture producers in EU, as it represents the deadline for implementing a full organic life cycle in the aquaculture production. Such a shift induces production costs for farmers and if it should be profitable, they must receive higher prices. This...... study identifies the price premium on organic salmon in the Danish retail sale sector using consumer panel scanner data for households by applying the hedonic price model while permitting unobserved heterogeneity between households. A premium of 20% for organic salmon is found. Since this premium is...... closer to organic labeled agriculture products than to ecolabelled capture fisheries products, it indicates that consumers value organic salmon as an agriculture product more than fisheries product....

  4. Isolation and characterization of Vibrio ( Listonella) anguillarum from catfish

    OpenAIRE

    Rad, Mehrnaz; Shahsavani, Davar

    2010-01-01

    Vibrio anguillarum was isolated from several organs of some aquarium catfish in a fish population with high mortality. Macroscopic examination of the affected catfish revealed ascites in the abdomen, site-petechiae, and dorsal erection. Although there are some variations in phenotypic characterization among different isolates of Vibrio anguillarum, the majority show a common biochemical profile with distinct microscopic appearance that can provide presumptive identification of Vibrio anguilla...

  5. Isolation, identification and characterization of regional indigenous Saccharomyces cerevisiae strains

    OpenAIRE

    Hana Šuranská; Dana Vránová; Jiřina Omelková

    2016-01-01

    Abstract In the present work we isolated and identified various indigenous Saccharomyces cerevisiae strains and screened them for the selected oenological properties. These S. cerevisiae strains were isolated from berries and spontaneously fermented musts. The grape berries (Sauvignon blanc and Pinot noir) were grown under the integrated and organic mode of farming in the South Moravia (Czech Republic) wine region. Modern genotyping techniques such as PCR-fingerprinting and interdelta PCR typ...

  6. Isolation, identification and characterization of regional indigenous Saccharomyces cerevisiae strains

    OpenAIRE

    Šuranská, Hana; Vránová, Dana; Omelková, Jiřina

    2016-01-01

    In the present work we isolated and identified various indigenous Saccharomyces cerevisiae strains and screened them for the selected oenological properties. These S. cerevisiae strains were isolated from berries and spontaneously fermented musts. The grape berries (Sauvignon blanc and Pinot noir) were grown under the integrated and organic mode of farming in the South Moravia (Czech Republic) wine region. Modern genotyping techniques such as PCR-fingerprinting and interdelta PCR typing were ...

  7. Untersuchungen der genetischen Varianz aktueller Isolate des porzinen Parvovirus

    OpenAIRE

    Zimmermann, Pia

    2003-01-01

    Studies on the genetic variability of recent isolates of porcine parvovirus The aim of this study was to review the antigenetic variability of porcine parvovirus and to examine the role of porcine parvovirus in reproductive failure. Organ specimens of 500 fetuses and stillborn piglets from a total of 172 farms were analysed. Total DNA was isolated from the explants and quantitative as well as qualitative PCR was subsequently carried out. In 82 samples (15,8%) out of 28 farms (16,3%), ...

  8. Spheres of isolation: adaptation of isolation levels to transactional workflow

    OpenAIRE

    Guabtni, Adnene; Charoy, François; Godart, Claude

    2005-01-01

    In Workflow Management Systems (WFMSs), transaction isolation is managed most of the time by the underlying database system using ANSI SQL strategies. These strategies do not take sufficiently into account process aspects. Our work consists in studying with more depth the relation between isolation strategy and process dimension as well as the real isolation needs in workflow environments. To carry out these needs, we define `spheres of isolation' inspired from `spheres of control' proposed b...

  9. Organic optoelectronics

    CERN Document Server

    Hu, Wenping; Gong, Xiong; Zhan, Xiaowei; Fu, Hongbing; Bjornholm, Thomas

    2012-01-01

    Written by internationally recognized experts in the field with academic as well as industrial experience, this book concisely yet systematically covers all aspects of the topic.The monograph focuses on the optoelectronic behavior of organic solids and their application in new optoelectronic devices. It covers organic electroluminescent materials and devices, organic photonics, materials and devices, as well as organic solids in photo absorption and energy conversion. Much emphasis is laid on the preparation of functional materials and the fabrication of devices, from materials synthesis a

  10. Organic synthesis

    International Nuclear Information System (INIS)

    The 1988 progress report of the Organic Synthesis Chemistry laboratory (Polytechnic School, France), is presented. The laboratory activities are centered on the chemistry of natural products, which have a biological activity and on the development of new reactions, useful in the organic synthesis. The research works involve the following domains: the natural products chemistry which are applied in pharmacology, the plants and insects chemistry, the organic synthesis, the radical chemistry new reactions and the bio-organic physicochemistry. The published papers, the congress communications and the thesis are listed

  11. Organic chemistry

    International Nuclear Information System (INIS)

    The activities of the mycotoxin research group are discussed. This includes the isolation and structure determination of mycotoxins, plant products, the biosyntheris of mycotoxins, the synthesis and characteristics of steroids, the synthesis and mechanistic aspects of heterocyclic chemistry and the functionality of steroids over long distances. Nmr spectra and mass spectroscopy are some of the techniques used

  12. Ultrasonic thermometer isolation standoffs

    International Nuclear Information System (INIS)

    A method is provided for minimizing sticking of the transmission line to the protective sheath and preventing noise echoes from interfering with signal echoes in an improved high temperature ultrasonic thermometer which includes an ultrasonic transmission line surrounded by a protective sheath. Small isolation standoffs are mounted on the transmission line to minimize points of contact between the transmission line and the protective sheath, the isolation standoffs serving as discontinuities mounted on the transmission line at locations where a signal echo is desired or where an echo can be tolerated. Consequently any noise echo generated by the sticking of the standoff to the protective sheath only adds to the amplitude of the echo generated at the standoff and does not interfere with the other signal echoes. 6 claims, 3 figures

  13. High voltage isolation transformer

    Science.gov (United States)

    Clatterbuck, C. H.; Ruitberg, A. P. (Inventor)

    1985-01-01

    A high voltage isolation transformer is provided with primary and secondary coils separated by discrete electrostatic shields from the surfaces of insulating spools on which the coils are wound. The electrostatic shields are formed by coatings of a compound with a low electrical conductivity which completely encase the coils and adhere to the surfaces of the insulating spools adjacent to the coils. Coatings of the compound also line axial bores of the spools, thereby forming electrostatic shields separating the spools from legs of a ferromagnetic core extending through the bores. The transformer is able to isolate a high constant potential applied to one of its coils, without the occurrence of sparking or corona, by coupling the coatings, lining the axial bores to the ferromagnetic core and by coupling one terminal of each coil to the respective coating encasing the coil.

  14. Electron isolation at ATLAS

    International Nuclear Information System (INIS)

    The ATLAS experiment at the Large Hadron Collider (LHC) will face the challenge of efficiently selecting interesting candidate events in pp collisions at 14 TeV centre-of-mass energy, whilst rejecting the enormous number of background events. Many of these interesting candidate events have isolated leptons in the final state, like for example events with a gauge boson or SUSY. On top of the standard ATLAS electron identification an isolation criterion has been developed using a likelihood as multivariate approach with several discriminating variables. The likelihood is constructed by selecting electrons from Z decays for the signal and for the background electrons from b quark jets. Results for the example of the associated Higgs boson production with top quarks and subsequent decay into a pair of W bosons are presented. In addition first results of a likelihood to discriminate against jets are given and a possible extension for muons is discussed

  15. Dynamic Isolated Domains

    OpenAIRE

    Nyman, Thomas

    2014-01-01

    Operating System-level Virtualization is virtualization technology based on running multiple isolated userspace instances, commonly referred to as containers, on top of a single operating system kernel. The fundamental difference compared to traditional virtualization is that the targets of virtualization in OS-level virtualization are kernel resources, not hardware. OS-level virtualization is used to implement Bring Your Own Device (BYOD) policies on contemporary mobile platforms. Current co...

  16. Isolated Pulmonary Valve Endocarditis

    Directory of Open Access Journals (Sweden)

    Mohammad Ali Hatamizadeh

    2009-06-01

    Full Text Available Infective endocarditis is one of the most severe complications of parenteral drug abuse. The outstanding clinical feature of infective endocarditis in intravenous drug abusers is the high incidence of right-sided valve infection, and the tricuspid valve is involved in 60% to 70% of the cases. We herein report a case of isolated pulmonic valve infective endocarditis with a native pulmonary valve.

  17. Galvanically Isolated Modular Converter

    OpenAIRE

    Christe, Alexandre; Dujic, Drazen

    2016-01-01

    Direct current (DC) electrical grids are already a reality in low voltage (LV) telecom distribution systems and point-to-point high voltage DC transmission. Medium voltage (MV) domain, despite its big potential, still suffers from a lack of suitable conversion and protection technologies. This study presents a bidirectional, galvanically isolated, high power converter for interface of emerging MVDC grids with readily available LVAC grids. To achieve high conversion efficiency, the integration...

  18. Napoleon in isolation

    OpenAIRE

    Calegari, Danny

    1999-01-01

    Napoleon's theorem in elementary geometry describes how certain linear operations on plane polygons of arbitrary shape always produce regular polygons. More generally, certain triangulations of a polygon that tiles R^2 admit deformations which keep fixed the symmetry group of the tiling. This gives rise to isolation phenomena in cusped hyperbolic 3-manifolds, where hyperbolic Dehn surgeries on some collection of cusps leaves the geometric structure at some other collection of cusps unchanged.

  19. Brown Fat Cell Isolation

    OpenAIRE

    sprotocols

    2014-01-01

    Author: C.R. Kahn ### 1.) ISOLATION AND PRIMARY CULTURE OF BROWN FAT PREADIPOCYTES ### Rationale: To prepare primary brown preadipocytes for immortalization: useful for metabolic studies from knockout mice. This consists of the following five protocols. References: Fasshauer, M., J. Klein, K M. Kriauciunas, K. Ueki, M.Benito, and C.R. Kahn. 2001. Essential role of insulin substrate 1 in differentiation of brown adipocytes. *Mol Cell Biol* 21: 319-329. Fasshauer, M....

  20. Isolated unilateral disk edema

    OpenAIRE

    Varner P

    2011-01-01

    Paul VarnerJohn J Pershing VAMC, Poplar Bluff, MO, USAAbstract: Isolated unilateral disk edema is a familiar clinical presentation with myriad associations. Related, non-consensus terminology is a barrier to understanding a common pathogenesis. Mechanisms for the development of disk edema are reviewed, and a new framework for clinical differentiation of medical associations is presented.Keywords: disk edema, axoplasmic flow, clinical multiplier, optic neuritis, ischemic optic neuropathy, papi...

  1. Mechanical beam isolator

    International Nuclear Information System (INIS)

    Back-reflections from a target, lenses, etc. can gain energy passing backwards through a laser just like the main beam gains energy passing forwards. Unless something blocks these back-reflections early in their path, they can seriously damage the laser. A Mechanical Beam Isolator is a device that blocks back-reflections early, relatively inexpensively, and without introducing aberrations to the laser beam

  2. Isolated Asymptomatic Short Sternum in a Healthy Young Girl

    Directory of Open Access Journals (Sweden)

    Francesco Turturro

    2014-01-01

    Full Text Available Congenital sternal defects are rare deformities frequently associated with other anomalies of the chest wall and other organ systems. Although pectus excavatum, pectus carinatum, and cleft sternum can present as isolated deformity, in most cases they are associated with heart and inner organs anomalies and described as symptoms of syndromes like Marfan syndrome, Noonan syndrome, Poland anomaly, and Cantrell pentalogy. In contrast, the etiology of an isolated defect is not well understood. We observed a short sternum (dysmorphic manubrium, hypoplastic body, and complete absence of the xiphoid process in a completely asymptomatic 13-year-old woman. A comprehensive instrumental exams panel was performed to exclude associated anomalies of the heart and of the other organ systems. The patient was completely asymptomatic and she did not need any medical or surgical treatment. To our knowledge, this is the first case of isolated short sternum reported in literature.

  3. First Australian isolation of epidemic Clostridium difficile PCR ribotype 027.

    Science.gov (United States)

    Riley, Thomas V; Thean, Sarah; Hool, Graham; Golledge, Clayton L

    2009-06-15

    We report the first isolation in Australia of a hypervirulent epidemic strain of Clostridium difficile, PCR ribotype 027. It was isolated from a 43-year-old woman with a permanent ileostomy, who appears to have been infected while travelling in the United States. The isolate was positive for toxin A, toxin B and binary toxin, and resistant to fluoroquinolone antimicrobials, and had characteristic deletions in the tcdC gene. All diagnostic laboratories and health care facilities in Australia should now be on high alert for this organism. PMID:19527210

  4. Standards for the testing and evaluation of seismic isolation systems

    Energy Technology Data Exchange (ETDEWEB)

    Taylor, A.W.; Chung, R.M. [National Inst. of Standards and Technology, Gaithersburg, MD (United States). Building and Fire Research Lab.; Shenton, H.W. [Univ. of Delaware, Newark, DE (United States). Dept. of Civil Engineering

    1995-12-01

    Draft guidelines for the testing and evaluation of seismic isolation systems have recently been developed at the National Institute of Standards and Technology (NIST). These guidelines are organized into three sections: pre-qualification, prototype, and quality control testing. The guidelines are broadly applicable, since they are independent of the type of isolation system and superstructure. The guidelines will serve as a resource document for industry, and as a basis for developing future standards for testing of isolation systems. This paper gives an overview of the NIST draft guidelines, emphasizing the philosophy behind the development of the guidelines. A brief summary of the contents of the guidelines is also presented.

  5. Isolation and characterization of deodorizing bacteria for organic sulfide malodor

    Institute of Scientific and Technical Information of China (English)

    JIANG An-xi; LIU Bo; ZHAO Yang-guo; LI Zheng; BAI Yu; CHENG Yang-xue

    2004-01-01

    Strain Jll screened out from different odor origins can efficiently degrade methyl mercaptan and ethanethiol whereas has no ability to remove dimethyl sulfide. The results indicated that the strain Jll breaks only the C-SH bond. The optimum temperature and pH of Jll are 20-30℃ and 6.0-8.3 respectively. A systematic identification method-16S rDNA gene sequence comparison, for deodorizing bacteria was carried out. The 16S rDNA gene sequence analysis of strain Jll showed the highest level of 97% homology to Rape rhizosphere.

  6. Protein profile of Chlamydophila abortus isolates from Kerala, India

    Directory of Open Access Journals (Sweden)

    Binu K Mani

    Full Text Available Chlamydiae are of microbiological interest because of their mode of interaction with eukaryotic host cells and their specialized life cycle with unique features of parasitism. Reports regarding prevalence of infections of Chlamydophila abortus, the causative organism for chlamydial abortions in livestock, was the basis of the study. Two isolates, one each from cattle and goat abortion along with a reference isolate, were used for characterization with Sodium Dodecyl Sulphate-Poly Acrylamide Gel Electrophoresis (SDS-PAGE. Elementary bodies infected Mc Coy cells, harvested from bottle cultures were disrupted by Teflon coated magnetic pellet. Urografin-76 diluted with Tris-Potassium hydrochloride was used for purification of Elementary bodies of Chlamydophila abortus organism. On protein estimation of Elementary bodies by Biuret method, all the three isolates revealed protein concentration between 500-1000 mg/100ml, which were sufficient for electrophoresis. Ten percent of resolving gel and five percent of stacking gel of polyacrylamide in which 10g of processed isolate samples along with standard protein marker and Mc Coy cell protein (control were electrophoresed. Using Alpha Imager Gel Documentation System, the protein bands were analyzed. Twelve bands each for local bovine isolate and reference isolate were noticed while only 10 bands were there in the caprine isolate. Additional bands of 148 kDa and 135 kDa were present in bovine isolate, compared to the reference isolate, while 152 kDa and 137 kDa bands were unique for caprine isolate. [Vet. World 2011; 4(10.000: 470-472

  7. Antibiotic susceptibility patterns among respiratory isolates of Gram-negative bacilli in a Turkish university hospital

    OpenAIRE

    Gonlugur Ugur; Bakici Mustafa; Akkurt Ibrahim; Efeoglu Tanseli

    2004-01-01

    Abstract Background Gram-negative bacteria cause most nosocomial respiratory infections. At the University of Cumhuriyet, we examined 328 respiratory isolates of Enterobacteriaceae and Acinetobacter baumanii organisms in Sivas, Turkey over 3 years. We used disk diffusion or standardized microdilution to test the isolates against 18 antibiotics. Results We cultured organisms from sputum (54%), tracheal aspirate (25%), and bronchial lavage fluid (21%). The most common organisms were Klebsiella ...

  8. Antibacterial susceptibility patterns for microbial isolates associated with infectious keratitis in horses: 63 cases (1986-1994).

    Science.gov (United States)

    Moore, C P; Collins, B K; Fales, W H

    1995-10-01

    Seventy-three aerobic bacterial isolates were cultured from 64 eyes of 63 horses with infectious keratitis. Forty-two (58%) of the organisms isolated initially were gram-positive (g+, 10 genera) and 31 (42%) were gram-negative (g-, 5 genera). After local antimicrobial treatment, repeat cultures from samples obtained from 15 eyes of hospitalized horses yielded 21 secondary bacterial isolates. Staphylococci spp and Streptococci spp were the most common g(+) isolates and accounted for 79% of g(+) organisms isolated initially. Antibiograms revealed ticarcillin to be the most efficacious antibiotic tested on g(+) organisms, with 28 of 30 (93%) being susceptible. Of commercially available topical ophthalmic antibiotics tested on g(+) organisms, erythromycin was the most efficacious, with 32 of 35 (91%) isolates being susceptible. Pseudomonas spp, Escherichia coli, and Acinetobacter spp accounted for 68% of g(-) organisms isolated initially. Gentamicin, tobramycin, polymyxin B, and neomycin were highly effective in vitro against initial g(-) isolates. Chloramphenicol was ineffective against g(+) and g(-) organisms isolated initially. A significantly (P ciprofloxacin. Overall, secondary g(-) isolates were more susceptible to ciprofloxacin, neomycin, tobramycin, or amikacin than to gentamicin. Fungi were isolated in 24 of 63 (38%) horses in the study. Twenty-five filamentous fungi and 2 yeasts were identified from 24 eyes.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:7559027

  9. Geological problems in radioactive waste isolation

    International Nuclear Information System (INIS)

    The problem of isolating radioactive wastes from the biosphere presents specialists in the fields of earth sciences with some of the most complicated problems they have ever encountered. This is especially true for high level waste (HLW) which must be isolated in the underground and away from the biosphere for thousands of years. Essentially every country that is generating electricity in nuclear power plants is faced with the problem of isolating the radioactive wastes that are produced. The general consensus is that this can be accomplished by selecting an appropriate geologic setting and carefully designing the rock repository. Much new technology is being developed to solve the problems that have been raised and there is a continuing need to publish the results of new developments for the benefit of all concerned. The 28th International Geologic Congress that was held July 9--19, 1989 in Washington, DC provided an opportunity for earth scientists to gather for detailed discussions on these problems. Workshop W3B on the subject, ''Geological Problems in Radioactive Waste Isolation -- A World Wide Review'' was organized by Paul A Witherspoon and Ghislain de Marsily and convened July 15--16, 1989 Reports from 19 countries have been gathered for this publication. Individual papers have been cataloged separately

  10. Geological problems in radioactive waste isolation

    Energy Technology Data Exchange (ETDEWEB)

    Witherspoon, P.A. (ed.)

    1991-01-01

    The problem of isolating radioactive wastes from the biosphere presents specialists in the fields of earth sciences with some of the most complicated problems they have ever encountered. This is especially true for high level waste (HLW) which must be isolated in the underground and away from the biosphere for thousands of years. Essentially every country that is generating electricity in nuclear power plants is faced with the problem of isolating the radioactive wastes that are produced. The general consensus is that this can be accomplished by selecting an appropriate geologic setting and carefully designing the rock repository. Much new technology is being developed to solve the problems that have been raised and there is a continuing need to publish the results of new developments for the benefit of all concerned. The 28th International Geologic Congress that was held July 9--19, 1989 in Washington, DC provided an opportunity for earth scientists to gather for detailed discussions on these problems. Workshop W3B on the subject, Geological Problems in Radioactive Waste Isolation -- A World Wide Review'' was organized by Paul A Witherspoon and Ghislain de Marsily and convened July 15--16, 1989 Reports from 19 countries have been gathered for this publication. Individual papers have been cataloged separately.

  11. Organ Transplantation

    Science.gov (United States)

    ... must be just and fair. Value judgments and economic considerations affect who is admitted to waiting lists and who gets available organs, calling into question the justice and fairness of the allocation system. It is essential to increase the supply of donated organs; one ...

  12. Organic Synthesis

    OpenAIRE

    Romea, Pedro

    2014-01-01

    Organic Synthesis is a one-semester course of the fourth year of the Chemistry Degree at the Universitat de Barcelona. This course covers the most important transformations in Organic Chemistry, including a short introduction to the Retrosynthetic Analysis. The aim is to provide a solid knowledge of the main reactions and their mechanism, which could later be improved during Master studies.

  13. Organ Facts

    Science.gov (United States)

    ... in certain types of transplants. Discover data and statistics for each center. Visit the OPTN's Organ DataSource now > I am looking for >> About organ allocation About UNOS Being a living donor Calculator - CPRA Calculator - KDPI Calculator - LAS Calculator - MELD ...

  14. Organ Transplantation

    Science.gov (United States)

    ... organs that can be transplanted include Heart Intestine Kidney Liver Lung Pancreas You often have to wait a long time for an organ transplant. Doctors must match donors to recipients to reduce the risk of transplant rejection. Rejection happens when your immune system attacks the ...

  15. Organic aerosols

    International Nuclear Information System (INIS)

    Organic aerosols scatter solar radiation. They may also either enhance or decrease concentrations of cloud condensation nuclei. This paper summarizes observed concentrations of aerosols in remote continental and marine locations and provides estimates for the sources of organic aerosol matter. The anthropogenic sources of organic aerosols may be as large as the anthropogenic sources of sulfate aerosols, implying a similar magnitude of direct forcing of climate. The source estimates are highly uncertain and subject to revision in the future. A slow secondary source of organic aerosols of unknown origin may contribute to the observed oceanic concentrations. The role of organic aerosols acting as cloud condensation nuclei (CCN) is described and it is concluded that they may either enhance or decrease the ability of anthropogenic sulfate aerosols to act as CCN

  16. Differentiation Between Two Isolates of Pythium ultimum var. ultimum Isolated from Diseased Plants in Two Different Continents

    Directory of Open Access Journals (Sweden)

    Hani M.A. Abdelzaher

    2010-01-01

    Full Text Available This study was undertaken to investigate the differences between two distinct isolates of Pythium ultimum var. ultimum, the diseased organisms of wheat and lettuce in agricultural fields in each of Egypt and Germany, respectively. Morphological study indicated that the Egyptian and German isolates comprised a single taxon, P. ultimum var. ultimum, by the current taxonomical keys. The isolates were morphologically distinguished as having smaller or larger sexual organs by the sizes of their antheridia and oogonia. The German isolate grew faster in the lower temperature range of 4-15°C whereas the Egyptian isolate grew faster in the higher temperature range of 25-37°C. Similarities in the sequence of the r-DNA-ITS including the 5.8S rDNA demonstrated evident genetic similarity at the species level between the two isolates and should be used for confirmation of identification of species of Pythium. The two isolates were not distinguishable by their pathogenicity to cucumber seedlings. The effect of temperature, hydrogen ion concentration and osmotic potential on oospores production and germination were studied. Oospores production and germination showed a similar manner, however, the Egyptian isolate produced more oospores than the German isolate. Identification of the Egyptian and German isolates of Pythium ultimum var. ultimum to the species level can be done using sequencing of r-DNA-ITS including the 5.8S rDNA, however, some morphological and physiological differences might present within the same species. This might be attributed to the effect of environmental factors and cultural conditions.

  17. First Isolation of a Giant Virus from Wild Hirudo medicinalis Leech: Mimiviridae isolation in Hirudo medicinalis

    OpenAIRE

    Mondher Boughalmi; Isabelle Pagnier; Sarah Aherfi; Philippe Colson; Didier Raoult; Bernard La Scola

    2013-01-01

    Giant viruses and amoebae are common in freshwater, where they can coexist with other living multicellular organisms. We screened leeches from the species Hirudo medicinalis for giant viruses. We analyzed five H. medicinalis obtained from Tunisia (3) and France (2). The leeches were decontaminated and then dissected to remove internal parts for co-culture with Acanthamoeba polyphaga. The genomes of isolated viruses were sequenced on a 454 Roche instrument, and a comparative genomics analysis ...

  18. Phosphate uptake kinetics by Acinetobacter isolates.

    Science.gov (United States)

    Pauli, A S; Kaitala, S

    1997-02-01

    Acinetobacter isolates from activated sludge treatment plants of forest industry were used as model organisms for polyphosphate accumulating bacteria to study excess phosphate uptake by the overplus phenomenon as well as luxury uptake of phosphate during growth. The initial, rapid phosphate uptake by the phosphorus-starved Acinetobacter isolates (the overplus phenomenon) followed the Michaelis-Menten model (maximum initial phosphate uptake rate 29 mg P g(-1) dry mass (DM) h(-1), half-saturation constant for excess phosphate uptake 17 mg P L(-1)). During the rapid uptake no growth was observed, but most cells contained polyphosphate granules. Also growth and luxury uptake of phosphate could be modeled with the Michaelis-Menten equation (maximum phosphate uptake rate 3.7-12 mg P g(-1) DM h(-1), half-saturation constant for growth 0.47-6.0 mg P L(-1), maximum specific growth rate 0.15-0.55 h(-1)). PMID:18633985

  19. Isolation of Pancreatic Islets from Nonhuman Primates.

    Science.gov (United States)

    Berman, Dora M

    2016-01-01

    Nonhuman primates (NHP) constitute a highly relevant pre-clinical animal model to develop strategies for beta cell replacement. The close phylogenetic and immunologic relationship between NHP and humans results in cross-reactivity of various biological agents with NHP cells, as well as a very similar cytoarchitecture between islets from human and NHP that is strikingly different from that observed in rodent islets. The composition and location of endocrine cells in human or NHP islets, randomly distributed and associated with blood vessels, have functional consequences and a predisposition for paracrine interactions. Furthermore, translation of approaches that proved successful in rodent models to the clinic has been limited. Consequently, data collected from NHP studies can form the basis for an IND submission to the FDA. This chapter describes in detail the key aspects for isolation of islets from NHP, from organ procurement up to assessment of islet function, comparing and emphasizing the similarities between isolation procedures for human and NHP islets. PMID:27586422

  20. Staphylococcus epidermidis isolated in 1965 are more susceptible to triclosan than current isolates.

    Directory of Open Access Journals (Sweden)

    Sissel Skovgaard

    Full Text Available Since its introduction to the market in the 1970s, the synthetic biocide triclosan has had widespread use in household and medical products. Although decreased triclosan susceptibility has been observed for several bacterial species, when exposed under laboratory settings, no in vivo studies have associated triclosan use with decreased triclosan susceptibility or cross-resistance to antibiotics. One major challenge of such studies is the lack of strains that with certainty have not been exposed to triclosan. Here we have overcome this challenge by comparing current isolates of the human opportunistic pathogen Staphylococcus epidermidis with isolates collected in the 1960s prior to introduction of triclosan to the market. Of 64 current S. epidermidis isolates 12.5% were found to have tolerance towards triclosan defined as MIC≥0.25 mg/l compared to none of 34 isolates obtained in the 1960s. When passaged in the laboratory in the presence of triclosan, old and current susceptible isolates could be adapted to the same triclosan MIC level as found in current tolerant isolates. DNA sequence analysis revealed that laboratory-adapted strains carried mutations in fabI encoding the enoyl-acyl carrier protein reductase isoform, FabI, that is the target of triclosan, and the expression of fabI was also increased. However, the majority of the tolerant current isolates carried no mutations in fabI or the putative promoter region. Thus, this study indicates that the widespread use of triclosan has resulted in the occurrence of S. epidermidis with tolerance towards triclosan and that the adaptation involves FabI as well as other factors. We suggest increased caution in the general application of triclosan as triclosan has not shown efficacy in reducing infections and is toxic to aquatic organisms.

  1. Beyond Insulation and Isolation

    DEFF Research Database (Denmark)

    Højlund, Marie Koldkjær

    2016-01-01

    insulation and isolation strategies to reduce measurable and perceptual noise levels. However, these strategies are unsuited to support the need to feel as an integral part of the shared hospital environment. This article suggests that the gap is intimately linked to a reductionist framework underlying the......Most research on the acoustic environment in the Western modern hospital identifies raised noise levels as the main causal explanation for the ranking of noise as critical stressors for patients, relatives and staffs. Therefore the most widely used strategies to tackle the problem in practice are...

  2. Isolation of RNP granules

    DEFF Research Database (Denmark)

    Jønson, Lars; Nielsen, Finn Cilius; Christiansen, Jan

    2011-01-01

    be regarded as a supramolecular assembly of RNA and protein, probably representing several overlapping post-transcriptional operons. The present protocol describes how RNP granules may be isolated by the transgenic expression of a 3X FLAG version of an RNA-binding protein under tetracycline control...... via the tetracycline receptor/operator complex. In this way, inclusion of an appropriate tetracycline concentration ensures expression of the tagged version at the endogenous level, and the 3X FLAG tag is a convenient "handle" for the subsequent immunoprecipitation by immobilized anti-FLAG antibody....

  3. Diabetes in Population Isolates

    DEFF Research Database (Denmark)

    Grarup, Niels; Moltke, Ida; Albrechtsen, Anders;

    2016-01-01

    Type 2 diabetes (T2D) is an increasing health problem worldwide with particularly high occurrence in specific subpopulations and ancestry groups. The high prevalence of T2D is caused both by changes in lifestyle and genetic predisposition. A large number of studies have sought to identify...... on glucose-stimulated plasma glucose, serum insulin levels, and T2D. The variant defines a specific subtype of non-autoimmune diabetes characterized by decreased post-prandial glucose uptake and muscular insulin resistance. These and other recent findings in population isolates illustrate the value...

  4. How isolated is Antarctica?

    Science.gov (United States)

    Clarke, Andrew; Barnes, David K A; Hodgson, Dominic A

    2005-01-01

    The traditional view of Antarctica and the surrounding Southern Ocean as an isolated system is now being challenged by the recent discovery at the Antarctic Peninsula of adult spider crabs Hyas areneus from the North Atlantic and of larvae of subpolar marine invertebrates. These observations question whether the well described biogeographical similarities between the benthic fauna of the Antarctic Peninsula and the Magellan region of South America result from history (the two regions were once contiguous), or from a previously unrecognized low level of faunal exchange. Such exchange might be influenced by regional climate change, and also exacerbated by changes in human impact. PMID:16701330

  5. Multicultural organizations

    DEFF Research Database (Denmark)

    Lauring, Jakob; Selmer, Jan

    2011-01-01

    In recent years the attention towards demographic diversity has increased among academics and practitioners dealing with organization management. Finding a possible connection between diversity and group performance has been the objective of much extant research. Still, however, results are...

  6. Colloidal organization

    CERN Document Server

    Okubo, Tsuneo

    2015-01-01

    Colloidal Organization presents a chemical and physical study on colloidal organization phenomena including equilibrium systems such as colloidal crystallization, drying patterns as an example of a dissipative system and similar sized aggregation. This book outlines the fundamental science behind colloid and surface chemistry and the findings from the author's own laboratory. The text goes on to discuss in-depth colloidal crystallization, gel crystallization, drying dissipative structures of solutions, suspensions and gels, and similar-sized aggregates from nanosized particles. Special emphas

  7. Organic superconductors

    International Nuclear Information System (INIS)

    The history of superconductivity is traced and the recent discovery of the property in organic materials is discussed. It has been shown that in those organic chemicals in which flat molecules are stacked like tiles, conditions are created in which electrons can flow unhindered. It is reasonable to believe that superconductivity at 20k is now a feasible goal though the dream of resistance-less conduction at room temperature still appears a remote dream. (UK)

  8. Organic Conductors

    DEFF Research Database (Denmark)

    Andersen, Jan Rud; Jacobsen, Claus S.; Rindorf, Grethe;

    1975-01-01

    2,3,6,7-Tetramethyl-1,4,5,8-tetraselenafulvalene reacts with 2,5-dimethyl-7,7′,8,8′-tetracyano-p-quinodi-methane to give a highly conducting organic solid.......2,3,6,7-Tetramethyl-1,4,5,8-tetraselenafulvalene reacts with 2,5-dimethyl-7,7′,8,8′-tetracyano-p-quinodi-methane to give a highly conducting organic solid....

  9. Organization Design

    OpenAIRE

    Milton Harris; Artur Raviv

    2002-01-01

    This paper attempts to explain organization structure based on optimal coordination of interactions among activities. The main idea is that each manager is capable of detecting and coordinating interactions only within his limited area of expertise. Only the CEO can coordinate company wide interactions. The optimal design of the organization trades off the costs and benefits of various configurations of managers. Our results consist of classifying the characteristics of activities and manager...

  10. Schizosaccharomyces isolation method

    Directory of Open Access Journals (Sweden)

    Benito Santiago

    2014-01-01

    Full Text Available This study discusses the optimization of a selective and differential medium which would facilitate the isolation of Schizosaccharomyces (a genus with a low incidence compared to other microorganisms to select individuals from this genus for industrial purposes, especially in light of the recent recommendation of the use of yeasts from this genus in the wine industry by the International Organisation of Vine and Wine, or to detect the presence of such yeasts, for those many authors who consider them food spoilers. To this end, we studied various selective differential agents based on the main physiological characteristics of these species, such as their high resistances to high concentrations of sugar, sulfur dioxide, sorbic acid, benzoic acid, acetic acid or malo ethanolic fermentation. This selective medium is based on the genus resistance to the antibiotic actidione and its high resistance to inhibitory agents such as benzoic acid. Malic acid was used as a differential factor due to the ability of this genus to metabolise it to ethanol, which allows detecting of the degradation of this compound. Lastly, the medium was successfully used to isolate strains of Schizosaccharomyces pombe from honey and honeycombs.

  11. Virtual Organizations: Beyond Network Organization

    Directory of Open Access Journals (Sweden)

    Liviu Gabriel CRETU

    2006-01-01

    Full Text Available One of the most used buzz-words in (e-business literature of the last decade is virtual organization. The term "virtual" can be identified in all sorts of combinations regarding the business world. From virtual products to virtual processes or virtual teams, everything that is “touched” by the computer’s processing power instantly becomes virtual. Moreover, most of the literature treats virtual and network organizations as being synonyms. This paper aims to draw a much more distinctive line between the two concepts. Providing a more coherent description of what virtual organization might be is also one of our intentions.

  12. Buoyancy effects of a growing, isolated dendrite

    Science.gov (United States)

    Canright, D.; Davis, S. H.

    1991-01-01

    The buoyancy effect of a growing isolated dendrite on the solidification process in the undercooling liquid material was investigated by developing an analytic solution to the growth/convection problem in powers of a buoyancy parameter G. The solution depends on the Prandtl number P and the Stefan number S (undercooling) for the local velocity and thermal fields and also the buoyant alteration of the interface shape. Results suggest that buoyancy effect for metals (low P) may be qualitatively different from that for organics (high P).

  13. Isolation of humic acids from leonardite

    Energy Technology Data Exchange (ETDEWEB)

    Shah, S.B.; Tartamella, T.L.; Lee, S. [Univ. of Akron, OH (United States). Dept. of Chemical Engineering; Kulik, C.J. [Ancon International, Newark, CA (United States)

    1996-12-31

    The primary interest in humic acid is its use as an effective fertilizer. Humic substances, found commonly in low-rank coals, enhance plant growth directly through positive physiological effects and indirectly by affecting the properties of the soil. Humic acids have traditionally been defined as the dark-colored organic matter that can be extracted from soil by dilute alkali and other reagents and which is insoluble in dilute acid. This paper discusses the isolation of humic acid from leonardite using the alkaline extraction method and the subsequent characterization using elemental analysis and infrared spectroscopy techniques. In this study, yields of more than 60% were obtained.

  14. Isolated Candida infection of the lung

    Directory of Open Access Journals (Sweden)

    Yousef Shweihat

    2015-01-01

    Full Text Available Candida pneumonia is a rare infection of the lungs, with the majority of cases occurring secondary to hematological dissemination of Candida organisms from a distant site, usually the gastrointestinal tract or skin. We report a case of a 77-year-old male who is life-long smoker with a history of rheumatoid arthritis and polymyalgia rheumatica, but did not take immunosuppressants for those conditions. Here, we present an extremely rare case of isolated pulmonary parenchymal Candida infection in the form pulmonary nodules without evidence of systemic disease which has only been described in a few previous reports.

  15. Isolation and characterization of regulatory peptides and bioactive compounds

    OpenAIRE

    Norberg, Åke

    2004-01-01

    Isolation of peptides and other bioactive compounds is an important and often necessary step to get the total information about their structures. This is demonstrated by a number of different characterizations in this thesis. Bioactive peptides and small organic molecules can act as signaling substances and messengers in multicellular organisms and are fundamental to higher forms of life. The following bioactive peptides and compounds were studied. 1) Different assays ca...

  16. [Microbiological isolates in patients with febrile neutropenia and hematological neoplasias].

    Science.gov (United States)

    Figuera Esparza, M; Carballo, M; Silva, M; Figueredo, A; Avilán, J

    2006-09-01

    We studied the frequency of culture isolation, type of microorganism isolated and local pattern of resistance in 309 adult febrile neutropenic inpatients with hematological neoplasm, who were hospitalized between January 1998 and December 2003, in Caracas University Hospital (Hospital Universitario de Caracas), in Venezuela. There were 576 febrile neutropenic episodes. Organisms were isolated in 41% of 940 cultures. The most common organisms involved were gram-negative bacilli (48%), followed by gram-positive cocci (35.1%), fungal (11.5%) and other agents (5.4%). Coagulase-negative staphylococci (22.4%) and Escherichia coli (13.4%) were the most isolated; 58.5% of 479 agents isolated had an antibiogram; 62.2% of coagulase-negative staphylococci and 23.1% coagulase-positive staphylococci were oxacillin-resistant. We did not find any vancomycin-resistant organisms. The gram-negative antimicrobial sensitivity for imipenem was 96.2%, cefepime 81%, and ceftazidime 57.5%. Tazobactam-piperacillin and sulbactam-cefoperazone were tested in 26% of antibiograms and had activity of 57.1% and 77.5%, respectively. Fungi were not characterized. Betalactamases producing gram-negative bacilli were found. In general, micro-biological identification and local vigilance of antibacterial resistance pattern must be done routinely in these patients, in order to improve empiric therapy guidelines. PMID:17099792

  17. Sulfated compounds from marine organisms.

    Science.gov (United States)

    Kornprobst, J M; Sallenave, C; Barnathan, G

    1998-01-01

    More than 500 sulfated compounds have been isolated from marine organisms so far but most of them originate from two phyla only, Spongia and Echinodermata. The sulfated compounds are presented according to the phyla they have been identified from and to their chemical structures. Biological activities, when available, are also given. Macromolecules have also been included in this review but without structural details. PMID:9530808

  18. Isolation, Characterization and Identification of Microalgae from the Red Sea

    KAUST Repository

    Luque Alanís, Patricio

    2013-05-01

    Eukaryotic microalgae from the Red Sea were isolated, characterized and identified with the purpose of building a culture collection that will serve future research activities in the area of industrial microbiology. Seven sampling locations were surveyed using an in-house designed isolation protocol. Microalgae enrichment was carried out in vitro using the streak plate method and fluorescence activated cell sorting approaches. Colonial and cellular microscopy, growth media preference assays, as well as temperature, pH and salinity tolerance tests were carried out to describe the isolates. DNA extraction, PCR amplification, template sequencing and in silico analyses were carried out to identify the isolates and arrange them in a proper phylogenetic description. In total, 129 isolates were obtained. From these, only 39 were selected for characterization given their increased ability of accumulating large amounts of biomass in solid and liquid media in relatively short periods of time. All of these have a green color, are unicellular, non-motile, photosynthetic organisms and have a cell size ranging from 5 to 8 µm. More than half of them showed growth preference in Walne media, followed by F/2, MN and BG-11 SW. Maximum temperature tolerance of all organisms was around 38 ºC, while optimum growth was observed close to 25 ºC. pH preference was diverse and three groups were identified: acidic (6), intermediate (8 - 9) and alkaline (> 10) growing isolates. Salinity tests showed an overall growth preference at 25 PSU, approximately 10 units lower than that found at the sampling stations. Most isolates showed diminished growth at high salinity and high pH, except for OS3S1b which grew well in both cases, and could be an interesting strain to study further. Twenty four isolates were related to Ulvophyceae sp. MBIC10591 by BLAST approaches with a maximum identity of 96 - 97%. A maximum likelihood phylogenetic tree was created for these isolates, relative to the BLAST hits

  19. Organic Computing

    CERN Document Server

    Würtz, Rolf P

    2008-01-01

    Organic Computing is a research field emerging around the conviction that problems of organization in complex systems in computer science, telecommunications, neurobiology, molecular biology, ethology, and possibly even sociology can be tackled scientifically in a unified way. From the computer science point of view, the apparent ease in which living systems solve computationally difficult problems makes it inevitable to adopt strategies observed in nature for creating information processing machinery. In this book, the major ideas behind Organic Computing are delineated, together with a sparse sample of computational projects undertaken in this new field. Biological metaphors include evolution, neural networks, gene-regulatory networks, networks of brain modules, hormone system, insect swarms, and ant colonies. Applications are as diverse as system design, optimization, artificial growth, task allocation, clustering, routing, face recognition, and sign language understanding.

  20. Isolation of Serratia fonticola from skin lesions in a Nile Crocodile (Crocodylus niloticus) with an associated septicaemia.

    Science.gov (United States)

    Garcia, Marta E; Lanzarot, Pilar; Costas, Eduardo; Lopez Rodas, Victoria; Marín, Mercedes; Blanco, Jose L

    2008-05-01

    This paper describes the first isolation of Serratia fonticola in a Nile Crocodile (Crocodylus niloticus). This organism was initially isolated from skin lesions and blood and subsequently from a variety of organs during necropsy. S. fonticola was confirmed as the pathogen causing the infection. PMID:17451977

  1. Genomics dataset of unidentified disclosed isolates.

    Science.gov (United States)

    Rekadwad, Bhagwan N

    2016-09-01

    Analysis of DNA sequences is necessary for higher hierarchical classification of the organisms. It gives clues about the characteristics of organisms and their taxonomic position. This dataset is chosen to find complexities in the unidentified DNA in the disclosed patents. A total of 17 unidentified DNA sequences were thoroughly analyzed. The quick response codes were generated. AT/GC content of the DNA sequences analysis was carried out. The QR is helpful for quick identification of isolates. AT/GC content is helpful for studying their stability at different temperatures. Additionally, a dataset on cleavage code and enzyme code studied under the restriction digestion study, which helpful for performing studies using short DNA sequences was reported. The dataset disclosed here is the new revelatory data for exploration of unique DNA sequences for evaluation, identification, comparison and analysis. PMID:27408929

  2. Isolated pauci-immune pulmonary capillaritis

    Directory of Open Access Journals (Sweden)

    Ashok Kumar Mehrotra

    2015-01-01

    Full Text Available A young house wife presented with low grade fever, cough, haemoptysis and SOB of unknown aetiology for 40 days duration. Respiratory system examination revealed diffuse crepts and rhonchi. Other organ system examination did not reveal any abnormality. X-ray chest PA view and CT thorax showed diffuse bilateral necrotising nodular lesions of various sizes with small pleural effusion. She also had low resting oxygen saturation with falling haematocrit. Her Serum was week positive for p-ANCA and negative for MPO-ANCA. Bronchoscopy revealed continuous bloody aspirates. We could not isolate any organisms in any of the specimens from her and she was unresponsive to any of the antibiotics either. Based on the clinical, laboratory data, radiological features and positive outcome to pulse therapy of methylprednisolone and cyclophosphamide, she was diagnosed as a case of IPIPC.

  3. ANTIMICROBIAL ACTIVITY OF LACTIC ACID BACTERIAL ISOLATES

    Directory of Open Access Journals (Sweden)

    Utkarsha S. Shivsharan

    2013-08-01

    Full Text Available Micro-organisms have tendency to produce antimicrobial substances which show biological activity against other kind of micro-organisms. This phenomenon of bacterial antagonism is observed in lactic acid bacteria with competitive advantages. The lactic acid bacteria are commonly present in many fermented products, fruits and milk products. The variety of antimicrobial substances produced by lactic acid bacteria showing good inhibition capacity include production of lactic acid, acetic acid, hydrogen peroxide, carbon dioxide, diacetyl and bacteriocin. Bacteriocins produced by lactic acid bacteria are the subject of intense research because of their antimicrobial activity against food born bacteria such as Listeria monocytogenes, staphylococcus aureus, Bacillus cereus, Clostridium botulinum and several others .Bacteriocins may be bacteriostatic or bactericidal with narrow or broad range of activity. The main of the study was to study the antimicrobial activity of such lactic acid bacterial isolates.

  4. Isolated pauci-immune pulmonary capillaritis.

    Science.gov (United States)

    Mehrotra, Ashok Kumar; Gupta, Prahlad Rai; Khublani, Trilok Kumar; Anupam; Soni, Shridha; Feroz, Asif

    2015-01-01

    A young house wife presented with low grade fever, cough, haemoptysis and SOB of unknown aetiology for 40 days duration. Respiratory system examination revealed diffuse crepts and rhonchi. Other organ system examination did not reveal any abnormality. X-ray chest PA view and CT thorax showed diffuse bilateral necrotising nodular lesions of various sizes with small pleural effusion. She also had low resting oxygen saturation with falling haematocrit. Her Serum was week positive for p-ANCA and negative for MPO-ANCA. Bronchoscopy revealed continuous bloody aspirates. We could not isolate any organisms in any of the specimens from her and she was unresponsive to any of the antibiotics either. Based on the clinical, laboratory data, radiological features and positive outcome to pulse therapy of methylprednisolone and cyclophosphamide, she was diagnosed as a case of IPIPC. PMID:25624599

  5. A hybrid base isolation system

    Energy Technology Data Exchange (ETDEWEB)

    Hart, G.C. [Univ. of California, Los Angeles, CA (United States); Lobo, R.F.; Srinivasan, M. [Hart Consultant Group, Santa Monica, CA (United States); Asher, J.W. [kpff Engineers, Santa Monica, CA (United States)

    1995-12-01

    This paper proposes a new analysis procedure for hybrid base isolation buildings when considering the displacement response of a base isolated building to wind loads. The system is considered hybrid because of the presence of viscous dampers in the building above the isolator level. The proposed analysis approach incorporates a detailed site specific wind study combined with a dynamic nonlinear analysis of the building response.

  6. Isolation and the parish ministry

    OpenAIRE

    Irvine, Andrew R.

    1989-01-01

    The purpose of this thesis was to examine the concept of isolation as it occurs within the profession of ministry. Isolation, for the purpose of this thesis, is defined social-psychologically. Within the field research isolation is considered as evidenced professionally, socially and spiritually. This study utilized as its sample base 200 hundred Church of Scotland ministers (15% of total population) which provided 159 usable responses to an extensive mail survey. The mai...

  7. The Challenge of Isolation. Professional Development--Your First Year

    Science.gov (United States)

    Cookson, Peter W., Jr.

    2005-01-01

    One of the ironies of teaching is that it is one of the most social occupations, but it is also one of the most isolating professions. Most schools are organized in an eggcrate manner, making professional collaborations difficult. In what other business would a professional not have easy access to a telephone? What other professional lives are…

  8. Isolation and Analysis of Essential Oils from Spices

    Science.gov (United States)

    O'Shea, Stephen K.; Von Riesen, Daniel D.; Rossi, Lauren L.

    2012-01-01

    Natural product isolation and analysis provide an opportunity to present a variety of experimental techniques to undergraduate students in introductory organic chemistry. Eugenol, anethole, and carvone were extracted from six common spices using steam-distillation and diethyl ether as the extraction solvent. Students assessed the purity of their…

  9. Brevibacterium casei isolated as a cause of relapsing peritonitis

    OpenAIRE

    Althaf, Mohammed Mahdi; Abdelsalam, Mohamed Said; Alsunaid, Mohammed Sunaid; Hussein, Maged Hassan

    2014-01-01

    We report a case of relapsing peritonitis in a 33-year-old woman on automated peritoneal dialysis. End-stage renal disease was secondary to systemic lupus erythematosus complicated with lupus nephritis. The organism isolated was Brevibacterium casei that was not readily identified, delaying appropriate management with an extended antibiotic course. Definite management of B casei peritonitis was peritoneal dialysis catheter removal.

  10. Safety organization

    International Nuclear Information System (INIS)

    After a rapid definition of a nuclear basis installation, the national organization of nuclear safety in France is presented, as also the main organizations concerned and their functions. This report shows how the licensing procedure leading to the construction and exploitation of such installations is applied in the case of nuclear laboratories of research and development: examinations of nuclear safety problems are carried out at different levels: - centralized to define the frame out of which the installation has not to operate, - decentralized to follow in a more detailed manner its evolution

  11. Isolation and identification of Pseudomonas azotoformans for induced calcite precipitation.

    Science.gov (United States)

    Heidari Nonakaran, Siamak; Pazhouhandeh, Maghsoud; Keyvani, Abdullah; Abdollahipour, Fatemeh Zahra; Shirzad, Akbar

    2015-12-01

    Biomineralization is a process by which living organisms produce minerals. The extracellular production of these biominerals by microbes has potential for various bioengineering applications. For example, crack remediation and improvement of durability of concrete is an important goal for engineers and biomineral-producing microbes could be a useful tool in achieving this goal. Here we report the isolation, biochemical characterization and molecular identification of Pseudomonas azotoformans, a microbe that produces calcite and which potentially be used to repair cracks in concrete structures. Initially, 38 bacterial isolates were isolated from soil and cements. As a first test, the isolates were screened using a urease assay followed by biochemical tests for the rate of urea hydrolysis, calcite production and the insolubility of calcite. Molecular amplification and sequencing of a 16S rRNA fragment of selected isolates permitted us to identify P. azotoformans as a good candidate for preparation of biotechnological concrete. This species was isolated from soil and the results show that among the tested isolates it had the highest rate of urea hydrolysis, produced the highest amount of calcite, which, furthermore was the most adhesive and insoluble. This species is thus of interest as an agent with the potential ability to repair cracks in concrete. PMID:26386580

  12. Bacteriological profile isolated from cases of otitis media diagnosed at tertiary care hospital of Gujarat, India

    Directory of Open Access Journals (Sweden)

    Kirankumar Navnitlal Patel

    2016-09-01

    Results: Out of 140 samples 114 (81.43% had positive culture. out of 114 cases, 64 (56.14% were male. Majority of the cases (32.45% were in the age group of 0-14 years. Out of 114 positive cases, single organism was isolated in 99 (86.8% cases. Out of total 116 strains 44 (37.9% were gram positive and 72 (62.1% were gram negative organisms. Among gram positive organisms, Staphylococcus aureus (63.63% was predominant organisms isolated followed by coagulase negative Staphylococci (20.45% and among gram negative organism Pseudomonas (69.44% was predominant organisms isolated followed by proteus species. Conclusions: Staphylococcus aureus and Pseudomonas sp. were found to be the common cause of otitis media in our study. [Int J Res Med Sci 2016; 4(9.000: 3891-3894

  13. Activity screening of plant growth promoting rhizobacteria isolated from alfalfa rhizosphere

    Directory of Open Access Journals (Sweden)

    shahla pashapour

    2016-03-01

    Full Text Available Introduction: Some rhizobacteria by various mechanisms influence plant growth as they are called plant growth promoting rhizobacteria (PGPR. Scientists identified some PGPR characters involved in promoting plant growth, while all these characters are not able to study. The aim of this study was to evaluate PGP activities of bacterial isolates, (45 isolates belonged to rhizobium and 2 bacterial isolates belonged to Pseudomonas fluorescens, which were isolated from alfalfa (Medicago sativa rhizosphere and root nodules grown around Zanjan. Materials and methods: These bacteria were isolated from alfalfa roots grown around Zinc industries in Zanjan province. After bacterial isolation and purification from root and soil samples, isolates were screened in vitro for plant growth promoting traits such as IAA (Indole Acetic Acid, ACC- deaminase (Amino Cyclopropan Carboxylate, HCN (Hydrogen Cyanide, siderophore, chitinase production and mineral and organic phosphate solubilization activities. Results: The results indicated that 43 bacterial isolates produced IAA (4.04- 4.95 μg/ml and 15 isolates produced ACC- deaminase (0.23- 1.05 μg/ml. Only one isolate (Rm66 produced high amount of HCN. Qualitative siderophore production was observed in 9 isolates. None of the isolates produced chitinase. Solubilization of mineral phosphate was commonly detected in 19 isolates (4.33- 5.86 μg/ml, and 15 isolates solubilized organic phosphate (1.66- 144.28 μg/ml. Discussion and conclusion: This study shows that most of the bacterial strains which isolated from alfalfa cultivated lands had PGP activities and also a good potential to increase plant growth after inoculation with to seeds as eco- friendly fertilizers.

  14. Organizing Process

    DEFF Research Database (Denmark)

    Hull Kristensen, Peer; Bojesen, Anders

    This paper invites to discuss the processes of individualization and organizing being carried out under what we might see as an emerging regime of change. The underlying argumentation is that in certain processes of change, competence becomes questionable at all times. The hazy characteristics of...

  15. Designing Organizations

    DEFF Research Database (Denmark)

    DESIGNING ORGANIZATIONS: 21st Century Approaches er en vigtig publikation inden for organisationsdesign. Fokus ligger på nye udviklinger inden for organisationsdesign og bogen hjælper med at skabe mere reflekterende forskining og stærkere empiriske analyser inden for dette vigtige felt inden for...

  16. Organic Nanowires

    DEFF Research Database (Denmark)

    Balzer, Frank; Schiek, Manuela; Al-Shamery, Katharina;

    Single crystalline nanowires from fluorescing organic molecules like para-phenylenes or thiophenes are supposed to become key elements in future integrated optoelectronic devices [1]. For a sophisticated design of devices based on nanowires the basic principles of the nanowire formation have to be...

  17. Enumeration and Characterization of Acidophilic Microorganisms Isolated from a Pilot Plant Stirred-Tank Bioleaching Operation

    OpenAIRE

    Okibe, Naoko; Gericke, Mariekie; Hallberg, Kevin B.; Johnson, D. Barrie

    2003-01-01

    Microorganisms were enumerated and isolated on selective solid media from a pilot-scale stirred-tank bioleaching operation in which a polymetallic sulfide concentrate was subjected to biologically accelerated oxidation at 45°C. Four distinct prokaryotes were isolated: three bacteria (an Acidithiobacillus caldus-like organism, a thermophilic Leptospirillum sp., and a Sulfobacillus sp.) and one archaeon (a Ferroplasma-like isolate). The relative numbers of these prokaryotes changed in the three...

  18. Analysis of Virulence of Clinical Isolates of Salmonella enteritidis In Vivo and In Vitro

    OpenAIRE

    Lu, Sangwei; Manges, Amee R.; Xu, Yisheng; Fang, Ferric C.; Riley, Lee W

    1999-01-01

    Salmonella enterica serotype Enteritidis (S. enteritidis) is a major food-borne pathogen, and its incidence among all Salmonella serotypes has increased dramatically in the last two decades. To study the virulence characteristics of clinical isolates of S. enteritidis, we determined the 50% lethal doses (LD50) in mice of isolates of two major phage types (4 and 8). Isolates of both phage types showed a wide range of LD50 after oral inoculation, varying from under 102 organisms to over 108 org...

  19. Evaluation of the bioremediation ability of the isolated white rot fungus on the textile effluents

    OpenAIRE

    Indira Priyadarsini* R; V Bhuvaneswari; K. Suresh Kumar

    2012-01-01

    Every one contributes to the environmental pollution by theiractivities either directly or indirectly and it is our moral responsibility to uncover a solution in order to come out of it also. Hence an attempt was made to isolate a white rot fungus with better remediation ability. The white rot fungus was isolated from decay wood sample and identified as Trametes hirsuta by molecular identification methods. The assessment of the bioremediation ability of the isolated organism was done with fou...

  20. Newly recognized Leptospira species ("Leptospira inadai" serovar lyme) isolated from human skin.

    OpenAIRE

    Schmid, G P; Steere, A. C.; Kornblatt, A N; Kaufmann, A. F.; Moss, C W; Johnson, R C; Hovind-Hougen, K.; Brenner, D. J.

    1986-01-01

    Leptospira strain 10, which represents a new Leptospira species, was isolated from a skin biopsy of a patient with Lyme disease. Although pathogenic for laboratory animals, the organism was not considered to have a significant role in the patient's illness.

  1. Antibiotic susceptibility patterns among respiratory isolates of Gram-negative bacilli in a Turkish university hospital

    Directory of Open Access Journals (Sweden)

    Gonlugur Ugur

    2004-08-01

    Full Text Available Abstract Background Gram-negative bacteria cause most nosocomial respiratory infections. At the University of Cumhuriyet, we examined 328 respiratory isolates of Enterobacteriaceae and Acinetobacter baumanii organisms in Sivas, Turkey over 3 years. We used disk diffusion or standardized microdilution to test the isolates against 18 antibiotics. Results We cultured organisms from sputum (54%, tracheal aspirate (25%, and bronchial lavage fluid (21%. The most common organisms were Klebsiella spp (35%, A. baumanii (27%, and Escherichia coli (15%. Imipenem was the most active agent, inhibiting 90% of Enterobacteriaceae and A. baumanii organisms. We considered approximately 12% of Klebsiella pneumoniae and 21% of E. coli isolates to be possible producers of extended-spectrum beta-lactamase. K. pneumoniae isolates of the extended-spectrum beta-lactamase phenotype were more resistant to imipenem, ciprofloxacin, and tetracycline in our study than they are in other regions of the world. Conclusions Our results suggest that imipenem resistance in our region is growing.

  2. GC-MS Quantitation and Identification of Bisphenol-A Isolated from Water

    Science.gov (United States)

    Mead, Ralph N.; Seaton, Pamela J.

    2011-01-01

    Isolation and identification of organic compounds is a necessary skill chemistry students must be able to do with proficiency. In this upper-level undergraduate laboratory, students isolate bisphenol-A (BPA; 4-4'-isopropylidenediphenol) from water using solid-phase extraction (SPE) followed by derivatization with analysis by GC-MS. The students…

  3. Seismic isolation for Advanced LIGO

    CERN Document Server

    Abbott, R; Allen, G; Cowley, S; Daw, E; Debra, D; Giaime, J; Hammond, G; Hammond, M; Hardham, C; How, J; Hua, W; Johnson, W; Lantz, B; Mason, K; Mittleman, R; Nichol, J; Richman, S; Rollins, J; Shoemaker, D; Stapfer, G; Stebbins, R

    2002-01-01

    The baseline design concept for a seismic isolation component of the proposed 'Advanced LIGO' detector upgrade has been developed with proof-of-principle experiments and computer models. It consists of a two-stage in-vacuum active isolation platform that is supported by an external hydraulic actuation stage. Construction is underway for prototype testing of a full-scale preliminary design.

  4. Seismic isolation for Advanced LIGO

    International Nuclear Information System (INIS)

    The baseline design concept for a seismic isolation component of the proposed 'Advanced LIGO' detector upgrade has been developed with proof-of-principle experiments and computer models. It consists of a two-stage in-vacuum active isolation platform that is supported by an external hydraulic actuation stage. Construction is underway for prototype testing of a full-scale preliminary design

  5. Isolated transfer of analog signals

    Science.gov (United States)

    Bezdek, T.

    1974-01-01

    Technique transfers analog signal levels across high isolation boundary without circuit performance being affected by magnetizing reactance or leakage inductance. Transfers of analog information across isolated boundary are made by interrupting signal flow, with switch, in such a manner as to produce alternating signal which is applied to transformer.

  6. Patients experience of source isolation

    DEFF Research Database (Denmark)

    Johansen, Kamilla; Pedersen, Didde; Kragbak, Nina;

    2013-01-01

    , Nursing education in Århus, Hedeager 2, 8200 Aarhus N, Denmark. Background: Medical treatment and care of patients with infections may include source isolation of the patient, to avoid spreading of the infection. However, isolation is a potential physiological and psychological stress factor to the....... Quality of Care: The patients felt abandoned and forgotten by the nurses, because of fewer visits and time limited communication. This led to emotions such as frustrations, insecurity and neglect. While isolated the patients felt it difficult to achieve contact and have an optimal relation with the nurses...... experience both positive and negative effects of source isolation, and areas of potential interest for developing new strategies to improve care of isolated patients were identified. Surprisingly, despite both verbal and written information, the information has not been understood by the patients. Repetitive...

  7. Improved Active Vibration Isolation Systems

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The control force, feedback gain, and actuator stroke of several active vibration isolation systems were analyzed based on a single-layer active vibration isolation system. The analysis shows that the feedback gain and actuator stroke cannot be selected independently and the active isolation system design must make a compromise between the feedback gain and actuator stroke. The performance of active isolation systems can be improved by the joint vibration reduction using an active vibration isolation system with an adaptive dynamic vibration absorber. The results show that the joint vibration reduction method can successfully avoid the compromise between the feedback gain and actuator stroke. The control force and the object vibration amplitude are also greatly reduced.

  8. Entangling Organizations

    DEFF Research Database (Denmark)

    Strand, Anete Mikkala Camille

    2016-01-01

    The chapter accounts for the process of becoming of a changed practice within the area of disability care in the Municipality of Aalborg in Denmark. Across a period of a few months in the fall of 2015 a group of employees across the organization and an action researcher from Aalborg University (the...... author) met and formed a research- practice group and across this period a revised model for cooperation emerged that – upon realization – would reconfigure the intra-play of all relevant areas of the organization involved in disability care. The model included the grasping of disability as dis/ability...... and thereby the model opened the possibility for reworking the binary of ability/disability to the benefit of restorying the citizen’s ability in the practices of changing the disability care....

  9. Entangled Organizations

    DEFF Research Database (Denmark)

    Strand, Anete Mikkala Camille

    The paper accounts for the process of becoming of a changed practice within the area of disability care in the Municipality of Aalborg in Denmark. Across a period of a few months in the fall of 2015 a group of employees across the organization and an action researcher from Aalborg University (the...... author) met and formed a research group and across this period a revised model for cooperation emerged that – upon realization – would reconfigure the intra-play of all relevant areas of the organization involved in disability care. The model included the grasping of disability as dis/ability and thereby...... of dis/ability care were developed over the course of the project and as a result a renewed material-discursive practice emerged as the recommended ‘modus operandi’ of dis/ability care. A recommendation entailing a ‘village-based-community of practice’ which would extend the organizational borders...

  10. Complete genome sequence of Corynebacterium pseudotuberculosis Cp31, isolated from an Egyptian buffalo

    DEFF Research Database (Denmark)

    Silva, Artur; Ramos, Rommel Thiago Jucá; Ribeiro Carneiro, Adriana; Cybelle Pinto, Anne; de Castro Soares, Siomar; Rodrigues Santos, Anderson; Silva Almeida, Sintia; Guimarães, Luis Carlos; Figueira Aburjaile, Flávia; Barbosa, Eudes; Alves Dorella, Fernanda; Souza Rocha, Flávia; Souza Lopes, Thiago; Kawasaki, Regiane; Gomes Sá, Pablo; da Rocha Coimbra, Nilson Antônio; Teixeira Cerdeira, Louise; Silvanira Barbosa, Maria; Cruz Schneider, Maria Paula; Miyoshi, Anderson; Selim, Salah Abdel Karim; Moawad, Mohamed Salah; Azevedo, Vasco

    2012-01-01

    Corynebacterium pseudotuberculosis is of major veterinary importance because it affects many animal species, causing economically significant livestock diseases and losses. Therefore, the genomic sequencing of various lines of this organism, isolated from different hosts, will aid in the developm......Corynebacterium pseudotuberculosis is of major veterinary importance because it affects many animal species, causing economically significant livestock diseases and losses. Therefore, the genomic sequencing of various lines of this organism, isolated from different hosts, will aid in the...

  11. Microwave scattering parameter imagery of an isolated canine kidney

    International Nuclear Information System (INIS)

    This paper describes a method for imaging biosystems using radiation in the microwave frequency range (3.9 GHz). The advantages of interrogation with microwave radiation are discussed in terms of its physiological significance. An electromechanical scanning system for imaging isolated organs and the subsequent image processing techniques are described. Imagery is presented for a phantom target and an isolated canine kidney. The kidney images are interpreted in terms of the known anatomical and functional organization of the organ. It does appear to be possible to distinguish cortex corticis corresponding to a high proportion of proximal and distal convoluted tubules, deeper cortical layers corresponding to a high population density of glomeri, a medullary outer zone corresponding to the loops of Henle as well as the collecting system, and the region of calyces/renal pelvis

  12. Estudo da motilidade espontânea de segmentos de cólon ascendente, transverso e descendente de ratos, em banhos fisiológicos para órgãos isolados Study of spontaneous motility of parts of colon in physiologic chamber for isolated organ, in rats

    Directory of Open Access Journals (Sweden)

    Francisco Rodrigues de Sales

    2004-01-01

    Full Text Available OBJETIVO: Identificar as diferenças na freqüência, amplitude de contrações, traçados negativos e forma das ondas. MÉTODOS: Fez-se um estudo da motilidade espontânea de segmentos de cólon ascendente, transverso e descendente de ratas, em banhos fisiológicos para órgãos isolados. RESULTADOS: Verificou-se grande variabilidade de freqüências e amplitudes das contrações registradas. Embora tenha havido predominância de contrações fortes no cólon descendente e de contrações menores no cólon transverso, todos os tipos de ondas foram encontrados nos três segmentos. CONCLUSÕES: O cólon apresenta motilidade muito variável, não permitindo identificar padrão característico inconfundível para cada um dos três segmentos, ascendente, transverso e descendente. O cólon transverso apresentou maior quantidade de traçados negativos, menor amplitude de contrações e menor freqüência de contrações. O cólon descendente apresentou o menor número de traçados negativos e a maior média de amplitude de contrações dos três segmentos.PURPOSE: The aim was to identify the difference in frequency, amplitude of contractions, negative tracing and shape of the waves. METHODS: We made one study of spontaneous motility of parts of colon from mouse, in physiologic chamber for isolated organ. RESULTS: It was found the biggest frequence variability and amplitude of contractions registered. Although there has been predominancy of strong contractions in the descending colon and weaker contractions in the transverse one, all kinds of waves were found in the three segments. CONCLUSIONS: The colon shows variable motility, which does not permit identify the unmistakable characteristic pattern for each of the three segments, ascendent, transverse and descending. The transverse colon shows a bigger quantity of negative traces, smaller amplitude of contractions and less frequency of contractions. The descending colon showed a smaller number of

  13. Organizing Violence

    OpenAIRE

    Singh, Smita; Greif, Avner; Bates, Robert

    2002-01-01

    In stateless societies, coercion is privately provided; violence is employed to engage in, and to defend against, predation. At best, violence results in mere redistribution; being destructive, it more often results in a loss of social welfare. When organized, however, violence can be socially productive; it can be employed to defend property rights, thereby strengthening the incentives to engage in productive activity. To explore how violence can be rendered a source of welfare, the authors ...

  14. LEARNING ORGANIZATIONS: PREREQUISITE FOR SUCCESSFUL TOURISM ORGANIZATIONS

    Directory of Open Access Journals (Sweden)

    Natasa Kraleva

    2011-06-01

    Full Text Available Nowadays, each and every organization, including the tourism organizations should be learning organizations. The globalization and the competition affect the process of working of tourism organizations, where only the organizations which have knowledge, and are learning organizations can succeed. In a time of constant change, tourism organizations can have a competitive advantage only if they can do something or offer something different than their competitors. Many organizations in order to enable the learning process, should firstly undertake changes in the organization design, the organizational culture and the leadership style. The paper discusses the relevance of implementing the process of learning organizations as a concept for successful tourism organizations.

  15. Microbiological and molecular characterization of Staphylococcus hominis isolates from blood.

    Directory of Open Access Journals (Sweden)

    Soraya Mendoza-Olazarán

    Full Text Available BACKGROUND: Among Coagulase-Negative Staphylococci (CoNS, Staphylococcus hominis represents the third most common organism recoverable from the blood of immunocompromised patients. The aim of this study was to characterize biofilm formation, antibiotic resistance, define the SCCmec (Staphylococcal Chromosomal Cassette mec type, and genetic relatedness of clinical S. hominis isolates. METHODOLOGY: S. hominis blood isolates (n = 21 were screened for biofilm formation using crystal violet staining. Methicillin resistance was evaluated using the cefoxitin disk test and the mecA gene was detected by PCR. Antibiotic resistance was determined by the broth microdilution method. Genetic relatedness was determined by pulsed-field gel electrophoresis (PFGE and SCCmec typed by multiplex PCR using two different methodologies described for Staphylococcus aureus. RESULTS: Of the S. hominis isolates screened, 47.6% (10/21 were categorized as strong biofilm producers and 23.8% (5/21 as weak producers. Furthermore, 81% (17/21 of the isolates were methicillin resistant and mecA gene carriers. Resistance to ampicillin, erythromycin, and trimethoprim was observed in >70% of isolates screened. Each isolate showed a different PFGE macrorestriction pattern with similarity ranging between 0-95%. Among mecA-positive isolates, 14 (82% harbored a non-typeable SCCmec type: eight isolates were not positive for any ccr complex; four contained the mec complex A ccrAB1 and ccrC, one isolate contained mec complex A, ccrAB4 and ccrC, and one isolate contained the mec complex A, ccrAB1, ccrAB4, and ccrC. Two isolates harbored the association: mec complex A and ccrAB1. Only one strain was typeable as SCCmec III. CONCLUSIONS: The S. hominis isolates analyzed were variable biofilm producers had a high prevalence of methicillin resistance and resistance to other antibiotics, and high genetic diversity. The results of this study strongly suggested that S. hominis isolates harbor

  16. LEARNING ORGANIZATIONS: PREREQUISITE FOR SUCCESSFUL TOURISM ORGANIZATIONS

    OpenAIRE

    Natasa Kraleva

    2011-01-01

    Nowadays, each and every organization, including the tourism organizations should be learning organizations. The globalization and the competition affect the process of working of tourism organizations, where only the organizations which have knowledge, and are learning organizations can succeed. In a time of constant change, tourism organizations can have a competitive advantage only if they can do something or offer something different than their competitors. Many organizations in order to ...

  17. Organic photovoltaics

    Science.gov (United States)

    Demming, Anna; Krebs, Frederik C.; Chen, Hongzheng

    2013-12-01

    Energy inflation, the constant encouragement to economize on energy consumption and the huge investments in developing alternative energy resources might seem to suggest that there is a global shortage of energy. Far from it, the energy the Sun beams on the Earth each hour is equivalent to a year's supply, even at our increasingly ravenous rate of global energy consumption [1]. But it's not what you have got it's what you do with it. Hence the intense focus on photovoltaic research to find more efficient ways to harness energy from the Sun. Recently much of this research has centred on organic solar cells since they offer simple, low-cost, light-weight and large-area flexible photovoltaic structures. This issue with guest editors Frederik C Krebs and Hongzheng Chen focuses on some of the developments at the frontier of organic photovoltaic technology. Improving the power conversion efficiency of organic photovoltaic systems, while maintaining the inherent material, economic and fabrication benefits, has absorbed a great deal of research attention in recent years. Here significant progress has been made with reports now of organic photovoltaic devices with efficiencies of around 10%. Yet operating effectively across the electromagnetic spectrum remains a challenge. 'The trend is towards engineering low bandgap polymers with a wide optical absorption range and efficient hole/electron transport materials, so that light harvesting in the red and infrared region is enhanced and as much light of the solar spectrum as possible can be converted into an electrical current', explains Mukundan Thelakkat and colleagues in Germany, the US and UK. In this special issue they report on how charge carrier mobility and morphology of the active blend layer in thin film organic solar cells correlate with device parameters [2]. The work contributes to a better understanding of the solar-cell characteristics of polymer:fullerene blends, which form the material basis for some of the most

  18. Evaluation of Wind Farm Controller based Fault Detection and Isolation

    DEFF Research Database (Denmark)

    Odgaard, Peter Fogh; Shafiei, Seyed Ehsan

    2015-01-01

    detection and isolation and fault tolerant control has previously been proposed. Based on this model, and international competition on wind farm FDI was organized. The contributions were presented at the IFAC World Congress 2014. In this paper the top three contributions to this competition are shortly......In the process of lowering cost of energy of power generated by wind turbines, some focus has been drawn towards fault detection and isolation and as well as fault tolerant control of wind turbines with the purpose of increasing reliability and availability of the wind turbines. Most modern wind...

  19. Isolated microorganisms from Iranian grapes and its derivatives

    Directory of Open Access Journals (Sweden)

    S Maulani

    2012-03-01

    Full Text Available Background and Objectives: The objective of this study was to monitor the microorganisms isolated from grapes and its derivative traditional products produced in Iran.Material and Methods: Four kinds of grapes cultivated summer of 2010 in vineyard of Takestan and also grape derived products from Shahrod, Hamedan and Takestan were used for this study. The samples were cultured in specific media to isolate the microorganisms that might grow on or pollute the products.Results: Species of bacteria and fungi isolated from 4 kinds of grapes cultivated in Takestan graveyards and also from 2 kinds of derived traditional products; grape sap and sour grape (abe-ghure locally named, were taken from Takestan, Shahrod and Hamedan cities. Also, bacteria Bacillus spp., Micrococcus spp., Clostridium spp., and fungus of Penicillium spp., and Aspergillus spp. were isolated.Conclusion: The isolated bacteria were common microorganisms that grow in soil or in the organic fertilizer and may appear from the environments that samples were collected. These bacteria were not pathogenic to human. The fungus isolated from the grapes may harm humans as they produce toxin. The results suggested that bacterial diversity on grapes and its derived traditional products are expected to be monitored and described in all Iranian graveyards as Iran has been known as one of the world’s biggest grape producers.

  20. ELASTOMERIC SEISMIC-PROTECTION ISOLATORS FOR BUILDINGS AND BRIDGES

    Institute of Scientific and Technical Information of China (English)

    Toshio Nishi; Nobuo Murota

    2013-01-01

    A giant earthquake of magnitude 9.0 occurred in Pacific Ocean off of Tohoku District Japan on March 11,2011.The highest seismic intensity of 7 in JMA scale was recorded in Miyagi.In the Tohoku district,around 230 buildings are seismically isolated (hereafter,SI) mainly by elastomeric isolators (seismic rubber bearings).According to the official survey reports by several organizations (for example[1]),the records of those buildings have verified the effectiveness of the seismic isolation.The response acceleration of the SI buildings was reduced by 30% to 50% of the input ground acceleration.Additionally,the difference of the conditions inside the room between SI and the fixed-base buildings was obvious as well as the damage in main structures of the buildings.The displacements of the isolators by the earthquake were around 200 mm according to the records of the instruments.As a result,the performance of SI buildings and the elastomeric isolators in the Tohoku District-Off the Pacific Ocean Earthquake 2011 were excellent,and the efficiency of the seismic isolation was verified by the records of many buildings in wide area.

  1. Multilocus Sequence Typing for Interpreting Blood Isolates of Staphylococcus epidermidis

    Directory of Open Access Journals (Sweden)

    Prannda Sharma

    2014-01-01

    Full Text Available Staphylococcus epidermidis is an important cause of nosocomial infection and bacteremia. It is also a common contaminant of blood cultures and, as a result, there is frequently uncertainty as to its diagnostic significance when recovered in the clinical laboratory. One molecular strategy that might be of value in clarifying the interpretation of S. epidermidis identified in blood culture is multilocus sequence typing. Here, we examined 100 isolates of this species (50 blood isolates representing true bacteremia, 25 likely contaminant isolates, and 25 skin isolates and the ability of sequence typing to differentiate them. Three machine learning algorithms (classification regression tree, support vector machine, and nearest neighbor were employed. Genetic variability was substantial between isolates, with 44 sequence types found in 100 isolates. Sequence types 2 and 5 were most commonly identified. However, among the classification algorithms we employed, none were effective, with CART and SVM both yielding only 73% diagnostic accuracy and nearest neighbor analysis yielding only 53% accuracy. Our data mirror previous studies examining the presence or absence of pathogenic genes in that the overlap between truly significant organisms and contaminants appears to prevent the use of MLST in the clarification of blood cultures recovering S. epidermidis.

  2. A broad review of the status of seismic isolation study in Japan

    International Nuclear Information System (INIS)

    In Japan, studies on seismic isolation technologies have been extensively, performed by a several organizations for the last decade, in order to apply them to fast reactors and thermal reactors. These programs have been managed by CRIEPl, JAPC, NUPEC, PNC the electric utilities and so on. Japanese major reactor manufacturers and construction companies have been participating in each program. Consequently, the base isolation technologies in Japan have been well-developed and are changing their stage from the basic studies to the integration for actual nuclear application. In this paper, the background, the current status and future perspective on the seismic isolation studies conducted by a several Japanese organizations are concisely described. (author)

  3. OBJECT-ORIENTED IN ORGANIZATION MANAGEMENT: ORGANIC ORGANIZATION

    OpenAIRE

    AmrBadr-El-Din

    2014-01-01

    In a time when virtual organizations are fast becoming a phenomenon, this paper aims to explore how IT-based approaches—and in specific the object-oriented (organic) approach—can be used to structure and facilitate the management of such virtual organizations. As with traditional organizations, a virtual organization needs a structure that is fast, reliable, adaptable and deterministic. In addition, such organizations need a time and cost-efficient structure to a...

  4. Status report on activities on seismic isolation in Italy

    International Nuclear Information System (INIS)

    buildings and studies are being started for the development of new and optimized bearing materials, analysis of different bearing types, seismic monitoring, and applications to energy production plants. Finally, tests have been planned for interface piping. Due to the high complexity and considerable cost of development work on seismic isolation, national and international collaborations are essential to optimize such studies: national co-operations have been established in Italy among several organizations, universities and companies, forming the Working Group on Seismic Isolation (GLIS); international collaborations exist with the USA and Japan. This paper summarizes the main features of the above-mentioned activities. More details on the studies [a] to [c] have been reported by separate technical papers. (author)

  5. Isolation and Characterization of Actinomycete Antagonists of a Fungal Root Pathogen †

    OpenAIRE

    Crawford, Don L.; Lynch, James M.; Whipps, John M.; Ousley, Margaret A.

    1993-01-01

    By use of selective media, 267 actinomycete strains were isolated from four rhizosphere-associated and four non-rhizosphere-associated British soils. Organic media with low nutrient concentrations were found to be best for isolating diverse actinomycetes while avoiding contamination and overgrowth of isolation media by eubacteria and fungi. While all isolates grew well at pHs 6.5 to 8.0, a few were unable to grow at pH 6.0 and a significant number failed to grow at pH 5.5. Eighty-two selected...

  6. History of deceased organ donation, transplantation, and organ procurement organizations.

    Science.gov (United States)

    Howard, Richard J; Cornell, Danielle L; Cochran, Larry

    2012-03-01

    The historical development of deceased organ donation, transplantation, and organ procurement organizations is reviewed. The concept of transplantation, taking parts from one animal or person and putting them into another animal or person, is ancient. The development of organ transplantation brought on the need for a source of organs. Although many early kidney transplants used kidneys from living donors, these donors could not satisfy the ever-growing need for organs, and extrarenal organs were recovered only from deceased donors. This need for organs to satisfy the great demand led to specialized organizations to identify deceased donors, manage them until recovery occurred, and to notify transplant centers that organs were available for their patients. The functions of these organ procurement organizations expanded to include other required functions such as education, accounting, and compliance with state and federal requirements. Because of the shortage of organs relative to the demand, lack of a unified organ allocation system, the perception that organs are a national resource and should be governed by national regulations, and to improve results of organ procurement organizations and transplant centers, the federal government has regulated virtually all phases of organ procurement and transplantation. PMID:22489438

  7. Isolation of rat adrenocortical mitochondria

    International Nuclear Information System (INIS)

    Highlights: ► A method for isolation of adrenocortical mitochondria from the adrenal gland of rats is described. ► The purified isolated mitochondria show excellent morphological integrity. ► The properties of oxidative phosphorylation are excellent. ► The method increases the opportunity of direct analysis of adrenal mitochondria from small animals. -- Abstract: This report describes a relatively simple and reliable method for isolating adrenocortical mitochondria from rats in good, reasonably pure yield. These organelles, which heretofore have been unobtainable in isolated form from small laboratory animals, are now readily accessible. A high degree of mitochondrial purity is shown by the electron micrographs, as well as the structural integrity of each mitochondrion. That these organelles have retained their functional integrity is shown by their high respiratory control ratios. In general, the biochemical performance of these adrenal cortical mitochondria closely mirrors that of typical hepatic or cardiac mitochondria.

  8. Development of base isolated APWR

    International Nuclear Information System (INIS)

    The applicability of a seismic-isolation device to an Advanced Pressurized Water Reactor (APWR) plant was investigated in an attempt to rationally achieve plant safety against earthquake and promote the standardization of plant design. Consequently, it was found that the seismic-isolation device is capable of significantly mitigating the effects of seismic force on the building. As a result, in addition to a reduction in building volume, and wall thickness, the optimal design of support structures for equipment and piping can be achieved while maintaining safety against earthquakes. Finally, it has been verified that the base-isolated APWR plant will have an advantage in construction costs even after taking into account the costs of providing the seismic-isolation device. (author)

  9. Isolation of rat adrenocortical mitochondria

    Energy Technology Data Exchange (ETDEWEB)

    Solinas, Paola [Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 (United States); Department of Medicine, Center for Mitochondrial Disease, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 (United States); Fujioka, Hisashi [Electron Microscopy Facility, Department of Pharmacology, Center for Mitochondrial Disease, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 (United States); Tandler, Bernard [Department of Biological Sciences, School of Dental Medicine, Center for Mitochondrial Disease, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 (United States); Hoppel, Charles L., E-mail: charles.hoppel@case.edu [Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 (United States); Department of Medicine, Center for Mitochondrial Disease, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 (United States)

    2012-10-12

    Highlights: Black-Right-Pointing-Pointer A method for isolation of adrenocortical mitochondria from the adrenal gland of rats is described. Black-Right-Pointing-Pointer The purified isolated mitochondria show excellent morphological integrity. Black-Right-Pointing-Pointer The properties of oxidative phosphorylation are excellent. Black-Right-Pointing-Pointer The method increases the opportunity of direct analysis of adrenal mitochondria from small animals. -- Abstract: This report describes a relatively simple and reliable method for isolating adrenocortical mitochondria from rats in good, reasonably pure yield. These organelles, which heretofore have been unobtainable in isolated form from small laboratory animals, are now readily accessible. A high degree of mitochondrial purity is shown by the electron micrographs, as well as the structural integrity of each mitochondrion. That these organelles have retained their functional integrity is shown by their high respiratory control ratios. In general, the biochemical performance of these adrenal cortical mitochondria closely mirrors that of typical hepatic or cardiac mitochondria.

  10. Isolated tubercles of some Palaeoscolecida

    NARCIS (Netherlands)

    Boogaard, van den M.

    1989-01-01

    Tubercles of Palaeoscolex? tenensis Kraft & Mergl, 1989 found as isolated phosphatic microfossils in Ordovician limestones are described. The probability that the phosphatic microfossils assigned to the genera Hadimopanella Gedik, 1977 and Milaculum Müller, 1973 represent tubercles of palaeoscolecid

  11. Organic photovoltaics

    Science.gov (United States)

    Demming, Anna; Krebs, Frederik C.; Chen, Hongzheng

    2013-12-01

    Energy inflation, the constant encouragement to economize on energy consumption and the huge investments in developing alternative energy resources might seem to suggest that there is a global shortage of energy. Far from it, the energy the Sun beams on the Earth each hour is equivalent to a year's supply, even at our increasingly ravenous rate of global energy consumption [1]. But it's not what you have got it's what you do with it. Hence the intense focus on photovoltaic research to find more efficient ways to harness energy from the Sun. Recently much of this research has centred on organic solar cells since they offer simple, low-cost, light-weight and large-area flexible photovoltaic structures. This issue with guest editors Frederik C Krebs and Hongzheng Chen focuses on some of the developments at the frontier of organic photovoltaic technology. Improving the power conversion efficiency of organic photovoltaic systems, while maintaining the inherent material, economic and fabrication benefits, has absorbed a great deal of research attention in recent years. Here significant progress has been made with reports now of organic photovoltaic devices with efficiencies of around 10%. Yet operating effectively across the electromagnetic spectrum remains a challenge. 'The trend is towards engineering low bandgap polymers with a wide optical absorption range and efficient hole/electron transport materials, so that light harvesting in the red and infrared region is enhanced and as much light of the solar spectrum as possible can be converted into an electrical current', explains Mukundan Thelakkat and colleagues in Germany, the US and UK. In this special issue they report on how charge carrier mobility and morphology of the active blend layer in thin film organic solar cells correlate with device parameters [2]. The work contributes to a better understanding of the solar-cell characteristics of polymer:fullerene blends, which form the material basis for some of the most

  12. Missed isolated posterior malleolar fractures

    OpenAIRE

    Ozler, Turhan; Guven, Melih; Onal, Ayberk; Ulucay, Cagatay; Beyzadeoglu, Tahsin; Altintas, Faik

    2014-01-01

    Objective:The aim of this study was to evaluate the injury mechanism and clinical and radiological results of the patients with isolated posterior malleolar fracture.Methods: Seven patients (5 male, 2 female; mean age: 32 years; range: 23-40) with a missed isolated posterior malleolar fracture were included in the study. All patients had initially been examined for an ankle sprain in the emergency room, where the initial plain radiographs did not show any abnormality. Due to the long lasting ...

  13. CHARACTERIZATION OF RUBBER DEGRADING ISOLATES

    Directory of Open Access Journals (Sweden)

    M. D. Chengalroyen

    2012-12-01

    Full Text Available Sixteen soil samples were screened for the presence of rubber degrading strains. Twenty-five strains displaying clear zone formation on latex agar plates were purified. Identification revealed that twenty three of these isolates were Streptomyces species, one Pseudonocardia species and one Methylibium species. The addition of carbon sources with the exception of tween 80 enhanced extracellular rubber biodegradation. Scanning electron microscopy revealed that the isolates were able to colonize and penetrate vulcanized glove rubber.

  14. CHARACTERIZATION OF RUBBER DEGRADING ISOLATES

    OpenAIRE

    M. D. Chengalroyen; Dabbs, E R

    2012-01-01

    Sixteen soil samples were screened for the presence of rubber degrading strains. Twenty-five strains displaying clear zone formation on latex agar plates were purified. Identification revealed that twenty three of these isolates were Streptomyces species, one Pseudonocardia species and one Methylibium species. The addition of carbon sources with the exception of tween 80 enhanced extracellular rubber biodegradation. Scanning electron microscopy revealed that the isolates were able to colonize...

  15. Base isolation of fluid containers

    Energy Technology Data Exchange (ETDEWEB)

    Kim, S. [Cygna Group Inc./ICF Kaiser International, Oakland, CA (United States)

    1995-12-01

    Fluid containers often constitute critical internal equipment in power plants. However, due to possible structure-equipment interaction effect they are particularly vulnerable during strong earthquake events. An effective technique for protecting fluid containers is base isolation. By deflecting the possible seismic input energy into the superstructure, base isolation can substantially reduce seismic demand on the containers, making it more cost effective than equivalent conventional design.

  16. Base isolation of fluid containers

    International Nuclear Information System (INIS)

    Fluid containers often constitute critical internal equipment in power plants. However, due to possible structure-equipment interaction effect they are particularly vulnerable during strong earthquake events. An effective technique for protecting fluid containers is base isolation. By deflecting the possible seismic input energy into the superstructure, base isolation can substantially reduce seismic demand on the containers, making it more cost effective than equivalent conventional design

  17. Evaluation of the bioremediation ability of the isolated white rot fungus on the textile effluents

    Directory of Open Access Journals (Sweden)

    Indira Priyadarsini* R

    2012-08-01

    Full Text Available Every one contributes to the environmental pollution by theiractivities either directly or indirectly and it is our moral responsibility to uncover a solution in order to come out of it also. Hence an attempt was made to isolate a white rot fungus with better remediation ability. The white rot fungus was isolated from decay wood sample and identified as Trametes hirsuta by molecular identification methods. The assessment of the bioremediation ability of the isolated organism was done with four textile effluent samples and the physico-chemical parameters namely colour, pH, TSS, TDS, COD, BOD, Total Hardness, level of Calcium, Magnesium, Chloride and Sulphate were evaluated. The change in the initial pH values of the four textile effluents after treatment with the isolated organism was not significant. All the other parameters were found to be reduced from their respective initial level after treatment with the isolated white rot fungus.

  18. Isolation and identification of iron ore-solubilising fungus

    Directory of Open Access Journals (Sweden)

    Damase Khasa

    2010-09-01

    Full Text Available Potential mineral-solubilising fungi were successfully isolated from the surfaces of iron ore minerals. Four isolates were obtained and identified by molecular and phylogenetic methods as close relatives of three different genera, namely Penicillium (for isolate FO, Alternaria (for isolates SFC2 and KFC1 and Epicoccum (for isolate SFC2B. The use of tricalcium phosphate (Ca3(PO42in phosphate-solubilising experiments confirmed isolate FO as the only phosphate solubiliser among the isolated fungi. The bioleaching capabilities of both the fungus and its spent liquid medium were tested and compared using two types of iron ore materials, conglomerate and shale, from the Sishen Iron Ore Mine as sources of potassium (K and phosphorus (P. The spent liquid medium removed more K (a maximum of 32.94% removal, from conglomerate, than the fungus (a maximum of 21.36% removal, from shale. However, the fungus removed more P (a maximum of 58.33% removal, from conglomerate than the spent liquid medium (a maximum of 29.25% removal, from conglomerate. The results also indicated a potential relationship between the removal of K or P and the production of organic acids by the fungus. A high production of gluconic acid could be related to the ability of the fungus to reduce K and P. Acetic, citric and maleic acids were also produced by the fungus, but in lower quantities. In addition, particle size and iron ore type were also shown to have significant effects on the removal of potassium and phosphorus from the iron ore minerals. We therefore conclude that the spent liquid medium from the fungal isolate FO can potentially be used for biobeneficiation of iron ore minerals.

  19. Draft for KINS Regulatory Guide on Seismic Isolation System of Nuclear Power Plant Structures

    International Nuclear Information System (INIS)

    Applying a seismic isolation system to NPP structures is being seriously considered and researched in several countries. To date, only a few NPPs including 4 units in France and 2 units in Republic of South Africa have adopted the seismic isolation systems. However, France recently adopted seismic isolation systems to research reactors under construction such as Jules Horowitze and ITER, and countries such as Japan and US are pushing forward to develop seismic isolation systems for commercial NPPs. Accordingly, regulatory organizations in the US and Japan such as US NRC and JNES have been performing researches for the preparation of regulatory guides on seismic isolation systems. Using the research results, JNES recently distributed a regulatory guide for a seismic isolation system. In Korea, the joint research team including KEPCO E and C, KHNP, KAERI, and EESK has performed a research on applying a seismic isolation system to the APR 1400 system since 2011. In response to this movement, Korea Institute of Nuclear Safety (KINS) is conducting an independent research project to provide a regulatory guide (RG) on seismic isolation systems for NPP structures. In this paper, the framework on a draft KINS RG addressing design, analysis, manufacture, installation, maintenance, and performance evaluation of isolators, isolation systems and isolated structures is presented. The draft RG has been developed based upon domestic and overseas industrial codes and standards, regulatory documents and the related research information available to date. As noticed, while the concept of seismic isolation is not new and the technique has been applied to thousands of civil engineering structures worldwide, a full scale application to an NPP structure is rare and technical guides for ensuring the safety is needed. In this paper, the framework on a draft KINS RG addressing design, analysis, manufacture, installation, maintenance, and performance evaluation of the isolators, isolation

  20. Draft for KINS Regulatory Guide on Seismic Isolation System of Nuclear Power Plant Structures

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Seihyun; Hyun, Changhun; Cho, Hohyun; Kim, Moonsoo; Im, Changbock [Korea Institute of Nuclear Safety, Daejeon (Korea, Republic of); Choi, Sanghyun [Univ. of Transportation, Uiwang (Korea, Republic of); Hong, Keejeung [Koomin Univ., Seoul (Korea, Republic of)

    2014-05-15

    Applying a seismic isolation system to NPP structures is being seriously considered and researched in several countries. To date, only a few NPPs including 4 units in France and 2 units in Republic of South Africa have adopted the seismic isolation systems. However, France recently adopted seismic isolation systems to research reactors under construction such as Jules Horowitze and ITER, and countries such as Japan and US are pushing forward to develop seismic isolation systems for commercial NPPs. Accordingly, regulatory organizations in the US and Japan such as US NRC and JNES have been performing researches for the preparation of regulatory guides on seismic isolation systems. Using the research results, JNES recently distributed a regulatory guide for a seismic isolation system. In Korea, the joint research team including KEPCO E and C, KHNP, KAERI, and EESK has performed a research on applying a seismic isolation system to the APR 1400 system since 2011. In response to this movement, Korea Institute of Nuclear Safety (KINS) is conducting an independent research project to provide a regulatory guide (RG) on seismic isolation systems for NPP structures. In this paper, the framework on a draft KINS RG addressing design, analysis, manufacture, installation, maintenance, and performance evaluation of isolators, isolation systems and isolated structures is presented. The draft RG has been developed based upon domestic and overseas industrial codes and standards, regulatory documents and the related research information available to date. As noticed, while the concept of seismic isolation is not new and the technique has been applied to thousands of civil engineering structures worldwide, a full scale application to an NPP structure is rare and technical guides for ensuring the safety is needed. In this paper, the framework on a draft KINS RG addressing design, analysis, manufacture, installation, maintenance, and performance evaluation of the isolators, isolation

  1. Geomorphology of Minnesota - Isolated Landform Structures

    Data.gov (United States)

    Minnesota Department of Natural Resources — Geomorphology of Minnesota - Isolated Landform Structures are essentially cartographic arcs representing isolated glacial features that were mapped in conjunction...

  2. Radioactive waste isolation in salt: peer review of Office of Nuclear Waste Isolation's Socioeconomic Program Plan

    International Nuclear Information System (INIS)

    The following recommendations have been abstracted from the body of this report. The Office of Nuclear Waste Isolation's Socioeconomic Program Plan for the Establishment of Mined Geologic Repositories to Isolate Nuclear Waste should be modified to: (1) encourage active public participation in the decision-making processes leading to repository site selection; (2) clearly define mechanisms for incorporating the concerns of local residents, state and local governments, and other potentially interested parties into the early stages of the site selection process. In addition, the Office of Nuclear Waste Isolation should carefully review the overall role that these persons and groups, including local pressure groups organized in the face of potential repository development, will play in the siting process; (3) place significantly greater emphasis on using primary socioeconomic data during the site selection process, reversing the current overemphasis on secondary data collection, description of socioeconomic conditions at potential locations, and development of analytical methodologies; (4) include additional approaches to solving socioeconomic problems. For example, a reluctance to acknowledge that solutions to socioeconomic problems need to be found jointly with interested parties is evident in the plan; (5) recognize that mitigation mechanisms other than compensation and incentives may be effective; (6) as soon as potential sites are identified, the US Department of Energy (DOE) should begin discussing impact mitigation agreements with local officials and other interested parties; and (7) comply fully with the pertinent provisions of NWPA

  3. Effect of irradiation on size of isolated liver mitochondria

    International Nuclear Information System (INIS)

    The authors study changes in hepatocyte mitochondria during exposure to some external factors, as reflected in the dynamics of changes in their size. Mitochondria were isolated from each of five organs of mice on two centrifuges (parallel procedures for the control and after gamma-irradiation of the animals). The time of the experiments was counted after irradiation. It is shown that the arithmetic mean values of the areas of isolated mitochondria under normal conditions are closely similar and vary within a narrow interval. The goodness of fit of the empirical distribution with theoretical distributions using Pearson's and Kolmogorov's tests, is tested. The parameters of gamma-law of distribution are shown. The statistical analysis of data on changes in size of isolated mouse liver mitochondria showed that irradiation leans to an increase in their size

  4. Isolated methylmalonic acidemia: a case report.

    Science.gov (United States)

    Es Sadki, Tarik; Badiou, Stéphanie; Boubal, Mathilde; Baleine, Julien; Sieso, Victor; Vallat, Catherine; Cristol, Jean-Paul; Vianey-Saban, Christine; Cambonie, Gilles

    2016-08-01

    Isolated methylmalonic acidemia (AMR) is an inborn error of metabolism due to an enzymatic deficit in methylmalonyl-CoA mutase. AMR lead to increased methylmalonic acid in plasma and urine without hyperhomocysteinemia. The clinical signs are recurrent episodes of ketoacidosis and bouts of vomiting, dehydration and mental retardation. These symptoms do not respond to the administration of vitamin B12. We report a case of a ten-months-old infant to whom the diagnosis was suspected in the presence of a metabolic acidosis, hyperammonemia, without hepatic impairment and ketosis. The chromatography of organic acids showed elevated methylmalonic acid levels. Molecular genetics allowed confirming the diagnosis of deficit in methylmalonyl-CoA mutase demonstrating the genetic abnormality of the gene MUT. PMID:27492701

  5. Isolated Uterine Metastasis of Invasive Ductal Carcinoma

    Directory of Open Access Journals (Sweden)

    Deniz Arslan

    2013-01-01

    Full Text Available Introduction. Most common metastasis sites of breast cancer are the lungs, bones, liver, and brain, whereas uterine involvement by metastatic breast disease is rare. Metastatic carcinoma of the uterus usually originates from other genital sites, most commonly being from the ovaries. Invasive lobular carcinoma spreads to gynecologic organs more frequently than invasive ductal carcinoma. Case Report. A 57-year-old postmenopausal woman was diagnosed with breast carcinoma 2 years ago and modified radical mastectomy was performed. Pathological examination of tumor revealed invasive ductal carcinoma, stage IIIc. She presented with abdominal pain and distension. Diagnostic workup and gynecologic examination revealed lesions that caused diffuse thickening of the uterus wall. Endometrial sampling was performed for confirmation of the diagnosis. She underwent total abdominal hysterectomy and bilateral salpingo-oophorectomy. Breast carcinoma metastases in endometrium and myometrium were confirmed histopathologically and immunohistochemically. Conclusion. We herein report the first case of isolated uterine patient who had invasive ductal carcinoma of breast.

  6. Sphingobium vermicomposti sp. nov., isolated from vermicompost.

    Science.gov (United States)

    Vaz-Moreira, Ivone; Faria, Cátia; Lopes, Ana R; Svensson, Liselott; Falsen, Enevold; Moore, Edward R B; Ferreira, António C Silva; Nunes, Olga C; Manaia, Célia M

    2009-12-01

    Strain VC-230(T) was isolated from homemade vermicompost produced from kitchen waste. The isolate was a Gram-negative-staining, catalase- and oxidase-positive, motile rod-shaped bacterium able to grow at 15-37 degrees C and pH 6-8. On the basis of 16S rRNA gene sequence analysis, strain VC-230(T) was determined to belong to the family Sphingomonadaceae by its clustering with type strains of the genus Sphingobium, with Sphingobium chlorophenolicum ATCC 33790(T) (97.7 %) and Sphingobium herbicidovorans DSM 11019(T) (97.4 %) as its closest neighbours. The polar lipid pattern, the presence of spermidine and ubiquinone 10, the predominance of the cellular fatty acids C(18 : 1)omega7c/9t/12t, C(16 : 1)omega7c and C(16 : 0) and the G+C content of the genomic DNA supported the affiliation of this organism to the genus Sphingobium. The phylogenetic, chemotaxonomic, phenotypic and DNA-DNA hybridization analyses verify that strain VC-230(T) represents a novel species, for which the name Sphingobium vermicomposti sp. nov. is proposed. The type strain is VC-230(T) (=CCUG 55809(T) =DSM 21299(T)). PMID:19643879

  7. Isolation of Amylase Producing Bacteria from Solar Salterns of Nellore District, Andhra Pradesh, India

    Directory of Open Access Journals (Sweden)

    Shameer Syed

    2013-03-01

    Full Text Available α-Amylases are a class of starch degrading enzymes catalyzing the hydrolysis of internal α-1,4-O-glycosidic bonds in polysaccharides. The following investigation were carried out to isolate haloalkaliphilic bacteria, a group of organisms with twin extremities of pH and salinity, capable of producing α-amylases from an artificial solar saltern. A total of 25 discrete colonies were isolated, 21 isolates showed amylase production. Among these 7 isolates produced amylase at extreme conditions such as salt, alkalinity and temperature. The isolates were characterized biochemically and also for other enzymes. From the results it is imperative that these isolates can be further studied to exploit them up to industrial scale.

  8. Isolation of listeria monocytogenes in neural forms of listeriosis and abortions in ruminants

    Directory of Open Access Journals (Sweden)

    Vidić Branka

    2006-01-01

    Full Text Available L. monocytogenes is a food borne pathogen capable of causing serious invasive diseases in humans and animals, including abortion, septicemia, meningitis and meningoencephalitis. Isolation of the agent is the most accurate diagnostic method in all situations of suspected L. monocytogenes infection. Direct isolation of L. monocytogenes is relatively simple in cases when the number of organisms is very large, such as septicaemic disease forms. Isolation is quite difficult if the agent is present in very small quantities, such as in encephalitis, or if the sample is highly contaminated. In this paper we presented the isolation of L. monocytogenes from clinical samples by using selective media for enrichment and isolation, combined with the cold enrichment technique. In our investigation we isolated L. monocytogenes from 18 of the total of 46 investigated tissue samples originating from animals with clinical diagnosis of listeriosis. We also presented the basic differential-diagnostic procedure in relation to the mimicking bacterial species.

  9. Hierarchical organization versus self-organization

    OpenAIRE

    Busseniers, Evo

    2014-01-01

    In this paper we try to define the difference between hierarchical organization and self-organization. Organization is defined as a structure with a function. So we can define the difference between hierarchical organization and self-organization both on the structure as on the function. In the next two chapters these two definitions are given. For the structure we will use some existing definitions in graph theory, for the function we will use existing theory on (self-)organization. In the t...

  10. The isolation, characterization and evaluation of Frankia strains

    Energy Technology Data Exchange (ETDEWEB)

    Normand, P.; Lalonde, M.; Fortin, J.A.; Chatarpaul, L.

    1984-01-01

    Osmium tetroxide (OsO/sub 4/) was used as the sterilizing agent to isolate the nitrogen-fixing endophyte Frankia from nodules of host plants. This treatment resulted in a pure culture collection of 250 Frankia isolates from several species of Alnus (crispa, rugosa, viridis, glutinosa, and serrulata) and from other actinorhizal species from Quebec. Frankia isolates from A. viridis and S. canadensis are reported in this document for the first time. Sugars from whole-cell hydrolysates of the organisms were analysed by gas liquid chromatography to identify strains previously screened on the basis of morphology and infectivity. This technique proved especially useful for those isolates whose morphology was atypical (ACN8, ArgN22, SCN9, and SCN10). It was demonstrated that sugar analysis (2-O-methy-D-mannose, in particular) can be important in resolving the taxonomy of Frankia. The nitrogen-fixing efficiency of some Frankia strains was evaluated by inoculating A. crispa. It was found that the efficiency of organisms was not influenced by the host from which they were first isolated. However, significant differences between some isolates of the same provenance occurred. It was also found that those strains of Frankia which do not sporulate were more effective in fixing nitrogen than those which do. It is proposed that the terms type P and type N be used to designate spore positive (Sp/sup +/) and spore negative (Sp/sup -/) Frankia strains, respectively. Further, it is recommended that sporulation type be used as a valid basis for species definition in the genus Frankia. 118 refs., 16 figs., 10 tabs.

  11. Isolation, identification and antimicrobial susceptibility of Pantoea (Enterobacter) agglomerans isolated from consumed powdered infant formula milk (PIF) in NICU ward: First report from Iran

    OpenAIRE

    Jalal Mardaneh; Mohammad Mehdi Soltan Dallal

    2013-01-01

    Background and Objectives Pantoea agglomerans is a Gram-negative rod in the Enterobacteriaceae family. It is reported as both commensal and opportunistic pathogen of animals and humans. This organism is potential candidates as powdered infant milk formula-borne opportunistic pathogen. The aim of our study was to perform isolation, identification and antimicrobial susceptibility pattern of Pantoea (Enterobacter) agglomerans strains isolated from consumed powdered infant formula milk (PIF) in N...

  12. An OXA-48-producing Escherichia coli isolated from a Danish patient with no hospitalization abroad

    DEFF Research Database (Denmark)

    Gedebjerg, Anne; Hasman, Henrik; Sorensen, Christian Moller; Wang, Mikala

    2015-01-01

    Carbapenemase-producing organisms are disseminating globally and are now emerging as a worrying threat in Scandinavia. Before August 2013, OXA-48-producing organisms had not been detected in Danish patients. Here we report the isolation of an ST746 OXA-48-producing Escherichia coli with the plasmid...... pOXA-48a carrying the bla(OXA-48) gene isolated from a Danish patient without history of hospitalization abroad. The patient reported tourist travel to Egypt and Turkey. The potential acquisition of carbapenemase-producing organisms by ingestion of contaminated food is discussed....

  13. TOXIGENICITY CHARACTERIZATION OF Clostridium perfringens FROM BOVINE ISOLATES

    Directory of Open Access Journals (Sweden)

    L. BALDASSI

    2002-01-01

    Full Text Available Clinical samples from 71 bovine from different Brazilian states were processed for the analysis of anaerobe organisms with emphasis on the isolation and characterization of Clostridium spp. From these, eighty-nine Clostridium perfringens strains were recovered: 32 from liver, 19 from intestinal contents, 14 from kidney, 6 from rumen, 5 from nervous system, 4 from bone marrow, 2 from udder tract, blood, spleen and lung, and one from muscle. Four reference Clostridium perfringens types A, B, C, and D were used as controls in this study. All isolates were cultivated in appropriate media, and after centrifugation the supernatant and sediment were separated. From pure supernatant post exopolysaccharide (EPS extraction, mouse toxigenicity tests were performed, determining protein and protein plus carbohydrate, respectively. ELISA was performed from sediments. The results showed that 51 (57.3% of the isolates were toxigenic to mice when inoculated by intraperitoneal route; bacteria from different organs had variable patterns of toxigenicity. Toxigenicity of EPS extracts was only expressed when protein concentration was 0.04 mg/mL and between 0.31 and 0.5 mg/mL for carbohydrate. Isolates were characterized as toxigenic when showing optimum protein and carbohydrate concentrations.

  14. Isolation, identification and characterization of regional indigenous Saccharomyces cerevisiae strains

    Science.gov (United States)

    Šuranská, Hana; Vránová, Dana; Omelková, Jiřina

    2016-01-01

    In the present work we isolated and identified various indigenous Saccharomyces cerevisiae strains and screened them for the selected oenological properties. These S. cerevisiae strains were isolated from berries and spontaneously fermented musts. The grape berries (Sauvignon blanc and Pinot noir) were grown under the integrated and organic mode of farming in the South Moravia (Czech Republic) wine region. Modern genotyping techniques such as PCR-fingerprinting and interdelta PCR typing were employed to differentiate among indigenous S. cerevisiae strains. This combination of the methods provides a rapid and relatively simple approach for identification of yeast of S. cerevisiae at strain level. In total, 120 isolates were identified and grouped by molecular approaches and 45 of the representative strains were tested for selected important oenological properties including ethanol, sulfur dioxide and osmotic stress tolerance, intensity of flocculation and desirable enzymatic activities. Their ability to produce and utilize acetic/malic acid was examined as well; in addition, H2S production as an undesirable property was screened. The oenological characteristics of indigenous isolates were compared to a commercially available S. cerevisiae BS6 strain, which is commonly used as the starter culture. Finally, some indigenous strains coming from organically treated grape berries were chosen for their promising oenological properties and these strains will be used as the starter culture, because application of a selected indigenous S. cerevisiae strain can enhance the regional character of the wines. PMID:26887243

  15. Isolation, identification and characterization of regional indigenous Saccharomyces cerevisiae strains.

    Science.gov (United States)

    Šuranská, Hana; Vránová, Dana; Omelková, Jiřina

    2016-01-01

    In the present work we isolated and identified various indigenous Saccharomyces cerevisiae strains and screened them for the selected oenological properties. These S. cerevisiae strains were isolated from berries and spontaneously fermented musts. The grape berries (Sauvignon blanc and Pinot noir) were grown under the integrated and organic mode of farming in the South Moravia (Czech Republic) wine region. Modern genotyping techniques such as PCR-fingerprinting and interdelta PCR typing were employed to differentiate among indigenous S. cerevisiae strains. This combination of the methods provides a rapid and relatively simple approach for identification of yeast of S. cerevisiae at strain level. In total, 120 isolates were identified and grouped by molecular approaches and 45 of the representative strains were tested for selected important oenological properties including ethanol, sulfur dioxide and osmotic stress tolerance, intensity of flocculation and desirable enzymatic activities. Their ability to produce and utilize acetic/malic acid was examined as well; in addition, H2S production as an undesirable property was screened. The oenological characteristics of indigenous isolates were compared to a commercially available S. cerevisiae BS6 strain, which is commonly used as the starter culture. Finally, some indigenous strains coming from organically treated grape berries were chosen for their promising oenological properties and these strains will be used as the starter culture, because application of a selected indigenous S. cerevisiae strain can enhance the regional character of the wines. PMID:26887243

  16. Isolation, identification and characterization of regional indigenous Saccharomyces cerevisiae strains

    Directory of Open Access Journals (Sweden)

    Hana Šuranská

    2016-03-01

    Full Text Available Abstract In the present work we isolated and identified various indigenous Saccharomyces cerevisiae strains and screened them for the selected oenological properties. These S. cerevisiae strains were isolated from berries and spontaneously fermented musts. The grape berries (Sauvignon blanc and Pinot noir were grown under the integrated and organic mode of farming in the South Moravia (Czech Republic wine region. Modern genotyping techniques such as PCR-fingerprinting and interdelta PCR typing were employed to differentiate among indigenous S. cerevisiae strains. This combination of the methods provides a rapid and relatively simple approach for identification of yeast of S. cerevisiae at strain level. In total, 120 isolates were identified and grouped by molecular approaches and 45 of the representative strains were tested for selected important oenological properties including ethanol, sulfur dioxide and osmotic stress tolerance, intensity of flocculation and desirable enzymatic activities. Their ability to produce and utilize acetic/malic acid was examined as well; in addition, H2S production as an undesirable property was screened. The oenological characteristics of indigenous isolates were compared to a commercially available S. cerevisiae BS6 strain, which is commonly used as the starter culture. Finally, some indigenous strains coming from organically treated grape berries were chosen for their promising oenological properties and these strains will be used as the starter culture, because application of a selected indigenous S. cerevisiae strain can enhance the regional character of the wines.

  17. Resistance of Mycobacterium chelonei-like organisms to formaldehyde.

    OpenAIRE

    Hayes, P S; McGiboney, D L; Band, J. D.; Feeley, J C

    1982-01-01

    Mycobacterium chelonei-like organisms have been isolated from patients in two outbreaks of peritonitis involving chronic peritoneal dialysis machines routinely disinfected with 2 to 3% formaldehyde. Susceptibility studies revealed that water-adapted M. chelonei-like organism strains could survive 2 h of exposure to 10% formaldehyde.

  18. Isolation and characterization of "Methanosphaera cuniculi" sp. nov.

    OpenAIRE

    Biavati, B.; Vasta, M; Ferry, J G

    1988-01-01

    A nonmotile, gram-positive, spherical organism was isolated from the intestinal tracts of rabbits. Both hydrogen and methanol were required for growth. No methane was produced from hydrogen-carbon dioxide, formate, acetate, methylamines, ethanol, or isopropanol. The optimum pH was 6.8, and the optimum temperature was 35 to 40 degrees C. The DNA G+C content is 23 mol%. The pseudomurein cell wall contained serine. These characteristics and the immunological fingerprinting results are consistent...

  19. TOXIGENICITY CHARACTERIZATION OF Clostridium perfringens FROM BOVINE ISOLATES

    OpenAIRE

    L. Baldassi; M.L. Barbosa; E. E. BACH; S.T. Iaria

    2002-01-01

    Clinical samples from 71 bovine from different Brazilian states were processed for the analysis of anaerobe organisms with emphasis on the isolation and characterization of Clostridium spp. From these, eighty-nine Clostridium perfringens strains were recovered: 32 from liver, 19 from intestinal contents, 14 from kidney, 6 from rumen, 5 from nervous system, 4 from bone marrow, 2 from udder tract, blood, spleen and lung, and one from muscle. Four reference Clostridium perfringens types A, B, C,...

  20. Intact individual heart cells isolated from human ventricular tissue.

    OpenAIRE

    Powell, T.; Sturridge, M F; Suvarna, S K; Terrar, D. A.; Twist, V. W.

    1981-01-01

    The intricate architecture of heart muscle, comprising irregularly shaped cells which interdigitate in a complex three-dimensional array, has often compromised clear interpretation of experimental data obtained from the whole organ. One approach to minimise some of the difficulties is to use individual muscle cells in suspension, and data have already been reported using myocytes isolated from mammalian ventricles. It is difficult, however, to extrapolate results obtained from animal tissues ...

  1. Identification and characterization of Salmonella isolates from captured house sparrows

    OpenAIRE

    MIRZAIE, Sara; Hassanzadeh, Mohammad; ASHRAFI, Iradj

    2010-01-01

    The objectives of this study were to determine the prevalence of Salmonella in a population of house sparrows, which are commonly found around poultry houses, and to characterize the obtained Salmonella isolates via serotyping, multiplex polymerase chain reaction (PCR), and antibiotic resistance analysis. Samples of visceral organs (gastrointestinal tract, liver, and heart) from 470 house sparrows were subjected to culture and the results show that 18 (3.8%) were positive for Salmonella. Of t...

  2. Brevibacterium casei isolated as a cause of relapsing peritonitis.

    Science.gov (United States)

    Althaf, Mohammed Mahdi; Abdelsalam, Mohamed Said; Alsunaid, Mohammed Sunaid; Hussein, Maged Hassan

    2014-01-01

    We report a case of relapsing peritonitis in a 33-year-old woman on automated peritoneal dialysis. End-stage renal disease was secondary to systemic lupus erythematosus complicated with lupus nephritis. The organism isolated was Brevibacterium casei that was not readily identified, delaying appropriate management with an extended antibiotic course. Definite management of B casei peritonitis was peritoneal dialysis catheter removal. PMID:24648477

  3. Isolation and characterization of lipid-associated and neurosecretory polypeptides

    OpenAIRE

    Stark, Margareta

    2000-01-01

    Lipid-interacting proteins play important roles in all living organisms. This thesis focuses on isolation and characterization of an enzyme in the triacylglycerol biosynthesis (phosphatidic acid phosphatase, PAP), hydrophobic polypeptides in bile, and polypeptides in cerebrospinal fluid. These fields constitute methodological challenges and mean development of suitable tools in between lipid and protein biochemistry. Two methods used to measure PAP activity were compared. I...

  4. Practical isolation of methyl mercury in natural waters

    Energy Technology Data Exchange (ETDEWEB)

    Schintu, M.; Kauri, T.; Contu, A.; Kudo, A.

    1987-12-01

    A simple method to isolate both organic and inorganic mercury in natural waters is described. The mercuric compounds were quantitatively extracted with dithizone from six different kinds of water spiked at nanogram levels with radioactive mercuric chloride and methylmercuric chloride. After the separation from the inorganic mercury with sodium nitrite, methyl mercury was transferred to aqueous medium with sodium thiosulfate. The method provides a high recovery of organic as well as inorganic mercury to an aqueous medium, prior to their determination by gold-trap cold vapor atomic absorption spectrophotometry. This method is easy, rapid, and inexpensive. Furthermore, the limited number of analytical steps should reduce loss and contamination.

  5. Practical isolation of methyl mercury in natural waters

    International Nuclear Information System (INIS)

    A simple method to isolate both organic and inorganic mercury in natural waters is described. The mercuric compounds were quantitatively extracted with dithizone from six different kinds of water spiked at nanogram levels with radioactive mercuric chloride and methylmercuric chloride. After the separation from the inorganic mercury with sodium nitrite, methyl mercury was transferred to aqueous medium with sodium thiosulfate. The method provides a high recovery of organic as well as inorganic mercury to an aqueous medium, prior to their determination by gold-trap cold vapor atomic absorption spectrophotometry. This method is easy, rapid, and inexpensive. Furthermore, the limited number of analytical steps should reduce loss and contamination

  6. Isolation of antimicrobial peptides and proteins from tomato

    Czech Academy of Sciences Publication Activity Database

    Králová, M.; Šanda, Miloslav; Macková, M.; Macek, Tomáš

    Praha : Institute of Organic Chemistry and Biochemistry AS CR, v. v. i, 2011 - (Slaninová, J.), s. 73-76 ISBN 978-80-86241-44-9. - (Collection Symposium Series. 13). [Biologically Active Peptides /12./. Praha (CZ), 27.04.2011-29.04.2011] Grant ostatní: GA ČR(CZ) GA522/09/1693; GA ČR(CZ) GD305/09/H008 Institutional research plan: CEZ:AV0Z40550506 Keywords : tomato * antimicrobial peptides * plant peptide isolation Subject RIV: CC - Organic Chemistry

  7. ISOLATION OF Rickettsia bellii FROM Amblyomma ovale AND Amblyomma incisum TICKS FROM SOUTHERN BRAZIL

    Directory of Open Access Journals (Sweden)

    Richard Pacheco

    2008-08-01

    Full Text Available Objective. To isolate and characterize rickettsiae from the ticks Amblyomma ovale and Amblyomma incisum collected in the state of São Paulo. Materials and methods. Adult, free-living A. ovale and A. incisum were collected in an Atlantic rainforest area in the state of São Paulo, Brazil. Each tick was tested using the hemolymph assay; samples from positive ticks were placed in shell vials in order to isolate rickettsiae and subsequently grown in Vero cells. Amplification of three rickettsial genes (gltA, htrA and ompA was attempted using polymerase chain reaction (PCR for each isolate obtained. Amplicons were subsequently sequenced. Results. A total of 388 A. incisum and 50 A. ovale were collected. Only one A. incisum and one A. ovale were hemolymph-test positive. Rickettsiae were successfully isolated from these ticks; however establishment in Vero cell culture was successful only for the isolate from A. ovale. Bacterial contamination in the first cell passage of the A. incisum isolate precluded successful isolation of the organism. PCR products were obtained with the gltA and htrA primers for the two isolates, but no product was obtained with the ompA primers. By BLAST analysis, partial gltA and htrA sequences of isolates from A. ovale and A. incisum were similar to the corresponding sequences of R. bellii. Conclusions. This is the first report of R. bellii infecting A. incisum and the first successful isolation from A. ovale.

  8. Bacteria isolated from amoebae/bacteria consortium

    Science.gov (United States)

    Tyndall, Richard L.

    1995-01-01

    New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  9. Solo doctors and ethical isolation.

    Science.gov (United States)

    Cooper, R J

    2009-11-01

    This paper uses the case of solo doctors to explore whether working in relative isolation from one's peers may be detrimental to ethical decision-making. Drawing upon the relevance of communication and interaction for ethical decision-making in the ethical theories of Habermas, Mead and Gadamer, it is argued that doctors benefit from ethical discussion with their peers and that solo practice may make this more difficult. The paper identifies a paucity of empirical research related to solo practice and ethics but draws upon more general medical ethics research and a study that identified ethical isolation among community pharmacists to support the theoretical claims made. The paper concludes by using the literary analogy of Soderberg's Doctor Glas to illustrate the issues raised and how ethical decision-making in relative isolation may be problematical. PMID:19880707

  10. Sympathicotomy for isolated facial blushing

    DEFF Research Database (Denmark)

    Licht, Peter Bjørn; Pilegaard, Hans K; Ladegaard, Lars

    2012-01-01

    Background. Facial blushing is one of the most peculiar of human expressions. The pathophysiology is unclear, and the prevalence is unknown. Thoracoscopic sympathectomy may cure the symptom and is increasingly used in patients with isolated facial blushing. The evidence base for the optimal level...... of targeting the sympathetic chain is limited to retrospective case studies. We present a randomized clinical trial. Methods. 100 patients were randomized (web-based, single-blinded) to rib-oriented (R2 or R2-R3) sympathicotomy for isolated facial blushing at two university hospitals during a 6-year...... between R2 and R2-R3 sympathicotomy for isolated facial blushing. Both were effective, and QOL increased significantly. Despite very frequent side effects, the vast majority of patients were satisfied. Surprisingly, many patients experienced mild recurrent symptoms within the first year; this should...

  11. VIBRATION ISOLATION SYSTEM PROBABILITY ANALYSIS

    Directory of Open Access Journals (Sweden)

    Smirnov Vladimir Alexandrovich

    2012-10-01

    Full Text Available The article deals with the probability analysis for a vibration isolation system of high-precision equipment, which is extremely sensitive to low-frequency oscillations even of submicron amplitude. The external sources of low-frequency vibrations may include the natural city background or internal low-frequency sources inside buildings (pedestrian activity, HVAC. Taking Gauss distribution into account, the author estimates the probability of the relative displacement of the isolated mass being still lower than the vibration criteria. This problem is being solved in the three dimensional space, evolved by the system parameters, including damping and natural frequency. According to this probability distribution, the chance of exceeding the vibration criteria for a vibration isolation system is evaluated. Optimal system parameters - damping and natural frequency - are being developed, thus the possibility of exceeding vibration criteria VC-E and VC-D is assumed to be less than 0.04.

  12. Streptomyces mangrovi sp. nov., isolated from mangrove forest sediment.

    Science.gov (United States)

    Yousif, Ghada; Busarakam, Kanungnid; Kim, Byung-Yong; Goodfellow, Michael

    2015-09-01

    A Streptomyces strain isolated from a mangrove sediment was classified using a polyphasic approach. The organism, isolate GY1(T), was found to have chemical and morphological properties typical of members of the genus Streptomyces. The isolate was shown to form a distinct phyletic line within the Streptomyces radiopugnans 16S rRNA gene subclade and to be closely related to the type strain of Streptomyces fenhuangensis (98.7 % similarity). It is also closely related to the type strain of Streptomyces bakulensis which was also closely related to members of the Streptomyces glaucosporus 16S rRNA gene subclade. Isolate GY1(T) was distinguished readily from the S. barkulensis type strain and from species classified in the S. radiopugnans clade using a combination of morphological and physiological properties, including a requirement for seawater for growth. Based on the genotypic and phenotypic data, it is proposed that isolate GY1(T) (=NCIMB 14980(T), NRRL B-69296(T)) be classified in the genus Streptomyces as Streptomyces mangrovi sp. nov. PMID:26187116

  13. Phenotypic and molecular characterisation of Brucella isolates from marine mammals

    Directory of Open Access Journals (Sweden)

    Bashiruddin John B

    2008-12-01

    Full Text Available Abstract Background Bacteria of the genus Brucella are the causative organisms of brucellosis in animals and man. Previous characterisation of Brucella strains originating from marine mammals showed them to be distinct from the terrestrial species and likely to comprise one or more new taxa. Recently two new species comprising Brucella isolates from marine mammals, B. pinnipedialis and B. ceti, were validly published. Here we report on an extensive study of the molecular and phenotypic characteristics of marine mammal Brucella isolates and on how these characteristics relate to the newly described species. Results In this study, 102 isolates of Brucella originating from eleven species of marine mammals were characterised. Results obtained by analysis using the Infrequent Restriction Site (IRS-Derivative PCR, PCR-RFLP of outer membrane protein genes (omp and IS711 fingerprint profiles showed good consistency with isolates originating from cetaceans, corresponding to B. ceti, falling into two clusters. These correspond to isolates with either dolphins or porpoises as their preferred host. Isolates originating predominantly from seals, and corresponding to B. pinnipedialis, cluster separately on the basis of IS711 fingerprinting and other molecular approaches and can be further subdivided, with isolates from hooded seals comprising a distinct group. There was little correlation between phenotypic characteristics used in classical Brucella biotyping and these groups. Conclusion Molecular approaches are clearly valuable in the division of marine mammal Brucella into subtypes that correlate with apparent ecological divisions, whereas conventional bioyping is of less value. The data presented here confirm that there are significant subtypes within the newly described marine mammal Brucella species and add to a body of evidence that could lead to the recognition of additional species or sub-species within this group.

  14. Seismic, shock, and vibration isolation 1991

    International Nuclear Information System (INIS)

    It covers a wide variety of topics, including shock and vibration isolation analysis methods, development of new isolation system, characterization of material properties of vibration isolation components, analytical/experimental methods, optimal isolation design, and isolation system design criteria and requirements. The structures considered included buildings, high-tech facilities, nuclear power plants, and pipe lines. The work reported here is representative of current research activities and contributed to the advancement of the state of the art in vibration isolation. THe editor hopes this volume will serve as a useful resource for research and design engineers and stimulate further interest in seismic, shock, and vibration isolation research

  15. Active Fault Isolation in MIMO Systems

    DEFF Research Database (Denmark)

    Niemann, Hans Henrik; Poulsen, Niels Kjølstad

    2014-01-01

    isolation is based directly on the input/output s ignals applied for the fault detection. It is guaranteed that the fault group includes the fault that had occurred in the system. The second step is individual fault isolation in the fault group . Both types of isolation are obtained by applying dedicated......Active fault isolation of parametric faults in closed-loop MIMO system s are considered in this paper. The fault isolation consists of two steps. T he first step is group- wise fault isolation. Here, a group of faults is isolated from other pos sible faults in the system. The group-wise fault...

  16. Is an isolated cleft lip an isolated anomaly?

    NARCIS (Netherlands)

    Deelder, J. D.; Breugem, C. C.; de Vries, Iris; de Bruin, M.; van der Molen, A. B. Mink; van der Horst, C. M. A. M.

    2011-01-01

    Introduction: It is well known that patients with cleft lip/palate or cleft palate can have associated anomalies. However, there is a relative paucity of information about the possible anomalies associated with an isolated cleft lip. A recent study (Vallino et al., 2008) showed that children with cl

  17. Completely Isolated? Health Information Seeking among Social Isolates

    Science.gov (United States)

    Askelson, Natoshia M.; Campo, Shelly; Carter, Knute D.

    2011-01-01

    To better target messages it is important to determine where people seek their health information. Interpersonal networks are a common way most people gather health information, but some people have limited networks. Using data from the 2004 General Social Survey (N = 984), we compared social isolates and nonisolates in their health…

  18. Isolated complete corpus callosal agenesis

    Directory of Open Access Journals (Sweden)

    Jaiganesh S, Venkateshwaran A, Naresh Kumar C, Rajasekhar KV

    2014-11-01

    Full Text Available Isolated complete corpus callosal agenesis is a rare entity. Usually this condition will be an associated finding in other syndromes. 3 month old male child came with complaints of deformed foot on both sides, not having a social smile and neck holding. Patient referred to the Radiology department for MRI brain which showed complete absence of corpus callosum, widely separated and parallely placed lateral ventricles, colpocephaly, high riding of 3rd ventricle and absence of cingulate gyrus and radial arrangement of gyri along the interhemispheric fissure. Hence it was reported as isolated complete corpus callosal agenesis and this article describes the Embryogenesis, anatomy, developmental anomalies and its clinical manifestations & prognosis.

  19. Soluble organic nanotubes for catalytic systems

    Science.gov (United States)

    Xiong, Linfeng; Yang, Kunran; Zhang, Hui; Liao, Xiaojuan; Huang, Kun

    2016-03-01

    In this paper, we report a novel method for constructing a soluble organic nanotube supported catalyst system based on single-molecule templating of core-shell bottlebrush copolymers. Various organic or metal catalysts, such as sodium prop-2-yne-1-sulfonate (SPS), 1-(2-(prop-2-yn-1-yloxy)ethyl)-1H-imidazole (PEI) and Pd(OAc)2 were anchored onto the tube walls to functionalize the organic nanotubes via copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. Depending on the ‘confined effect’ and the accessible cavity microenvironments of tubular structures, the organic nanotube catalysts showed high catalytic efficiency and site-isolation features. We believe that the soluble organic nanotubes will be very useful for the development of high performance catalyst systems due to their high stability of support, facile functionalization and attractive textural properties.

  20. Database Replication Using Generalized Snapshot Isolation

    OpenAIRE

    Elnikety, Sameh; Pedone, Fernando; Zwaenepoel, Willy

    2005-01-01

    Generalized snapshot isolation extends snapshot isolation as used in Oracle and other databases in a manner suitable for replicated databases. While (conventional) snapshot isolation requires that transactions observe the “latest” snapshot of the database, generalized snapshot isolation allows the use of “older” snapshots, facilitating a replicated implementation. We show that many of the desirable properties of snapshot isolation remain. In particular, read-only transactions never block or a...

  1. Pelvic Organ Prolapse

    Science.gov (United States)

    ... Procedures Implants and Prosthetics Urogynecologic Surgical Mesh Implants Pelvic Organ Prolapse (POP) Share Tweet Linkedin Pin it More sharing ... Treatment Options for Pelvic Organ Prolapse? What is Pelvic Organ Prolapse? Pelvic organ prolapse (POP) occurs when the tissue ...

  2. Clostripain, the Missing Link in the Enzyme Blend for Efficient Human Islet Isolation

    Science.gov (United States)

    Ståhle, Magnus; Foss, Aksel; Gustafsson, Bengt; Lempinen, Marko; Lundgren, Torbjörn; Rafael, Ehab; Tufveson, Gunnar; Korsgren, Olle; Friberg, Andrew

    2015-01-01

    Background Effective digestive enzymes are crucial for successful islet isolation. Supplemental proteases are essential as they synergize with collagenase for effective pancreas digestion. The presence of tryptic-like activity has been implicated in efficient enzyme blends and the present study aimed to evaluate if addition of clostripain, an enzyme with tryptic-like activity, could improve efficacy of the islet isolation procedure. Methods Clostripain was added to the enzyme blend just before pancreas perfusion. Islets were isolated per standard method and numerous isolation parameters, islet quality control, and the number of isolations fulfilling standard transplantation criteria were evaluated. Two control organs per clostripain organ were chosen by blindly matching against body mass index, cold ischemia time, hemoglobin A1c, donor sex, and donor age. Results There were no differences in pancreas weight, dissection time, digestion time, harvest time, percent digested pancreas, or total pellet volume before islet purification between control or clostripain pancreases. Glucose-stimulated insulin release results were similar between groups. Total isolation islet equivalents, purified tissue volume and islet equivalents/g pancreas as well as fulfillment of transplantation criteria favored clostripain processed pancreases. Conclusions The addition of clostripain to the enzyme blend soundly improved islet yields and transplantation rates. It gently aided pancreas digestion and maintained proper islet functionality. The addition of clostripain to the enzyme blend has now been implemented into standard isolation protocols at the isolation centers in Uppsala and in Oslo.

  3. Organic plant breeding

    OpenAIRE

    Wolfe, Martin S.

    2002-01-01

    This report was presented at the UK Organic Research 2002 Conference. To a major extent, organic farming depends currently on plant and animal varieties that have been bred for non-organic farming and that are often not suited to organic production. This position is inconsistent with a holistic approach to organic agriculture. Organically-bred plant varieties are needed to develop both the potential of organic agriculture and its integrity. A first attempt at developing a concept for organic ...

  4. Effectiveness of Base Isolation Technique and Influence of Isolator Characteristics on Response of a Base Isolated Building

    Directory of Open Access Journals (Sweden)

    Sunita Tolani

    2016-06-01

    Full Text Available This study concerns with the seismic response comparison of a fixed base building with a base isolated building and parametric study of a base isolated building. The structural system considered for analysis is a three storey reinforced concrete building, which is idealized as a shear type building with one lateral degree of freedom at each floor level. The isolation systems considered for this study are Laminated Rubber bearing (LRB, Lead Rubber Bearing (N-Z bearing and Friction Pendulum System (FPS. The response of fixed base building and of base isolated building is compared in terms of maximum top floor acceleration, interstorey drift, maximum floor displacements and base shear. For parametric study important isolation system parameters considered are: (i isolation time period, isolator damping for LRB; (ii isolator yield strength, isolation time period, isolator damping for N-Z bearing and (iii isolation time period, friction coefficient for FPS. It is found that base isolation technique is very effective in reducing seismic response of structure and isolation system parameters significantly influence the earthquake response of a base isolated structure.

  5. Genetic Diversity of Mycobacterium avium Isolates Recovered from Clinical Samples and from the Environment: Molecular Characterization for Diagnostic Purposes▿

    OpenAIRE

    Álvarez, Julio; García, Ignacio Gómez; Aranaz, Alicia; Bezos, Javier; Romero, Beatriz; de Juan, Lucía; Mateos, Ana; Gómez-Mampaso, Enrique; Domínguez, Lucas

    2008-01-01

    Isolation of Mycobacterium avium complex (MAC) organisms from clinical samples may occur in patients without clinical disease, making the interpretation of results difficult. The clinical relevance of MAC isolates from different types of clinical samples (n = 47) from 39 patients in different sections of a hospital was assessed by comparison with environmental isolates (n = 17) from the hospital. Various methods for identification and typing (commercial probes, phenotypic characteristics, PCR...

  6. Isolation of mitochondria from CNS

    OpenAIRE

    Kristian, Tibor

    2010-01-01

    This unit contains a protocol describing the isolation of brain mitochondria by using discontinuous Percoll gradient centrifugation. The Percoll density gradient centrifugation separates synaptosomes, myelin, and free non-synaptic mitochondria released from cells during tissue homogenization into individual fractions. Mitochondria entrapped in synaptosomes (synaptic mitochondria) can be liberated using nitrogen cavitation and then further purified by Percoll gradient centrifugation. These met...

  7. Emotional isolation in BBC Forum

    International Nuclear Information System (INIS)

    We analyze emotionally annotated massive data from BBC Forum and examine properties of the isolation phenomenon of negative and positive users. Our results show the existence of a percolation threshold dependent on the average emotional value in the network of negatively charged nodes

  8. High-Voltage Isolation Transformer

    Science.gov (United States)

    Clatterbuck, C. H.; Ruitberg, A. P.

    1985-01-01

    Arcing and field-included surface erosion reduced by electrostatic shields around windings and ferromagnetic core of 80-kilovolt isolation transformer. Fabricated from high-resistivity polyurethane-based material brushed on critical surfaces, shields maintained at approximately half potential difference of windings.

  9. Emotional isolation in BBC Forum

    Science.gov (United States)

    Sienkiewicz, J.; Chmiel, A.

    2014-03-01

    We analyze emotionally annotated massive data from BBC Forum and examine properties of the isolation phenomenon of negative and positive users. Our results show the existence of a percolation threshold dependent on the average emotional value in the network of negatively charged nodes.

  10. Isolated tubercles of some Palaeoscolecida

    OpenAIRE

    Boogaard, van den, F.E.

    1989-01-01

    Tubercles of Palaeoscolex? tenensis Kraft & Mergl, 1989 found as isolated phosphatic microfossils in Ordovician limestones are described. The probability that the phosphatic microfossils assigned to the genera Hadimopanella Gedik, 1977 and Milaculum Müller, 1973 represent tubercles of palaeoscolecidans is discussed. A relation between Palaeoscolecida and Chordata is hinted at.

  11. Screening for Anti-Cancer Compounds in Marine Organisms in Oman

    OpenAIRE

    Sergey Dobretsov; Yahya Tamimi; Al-Kindi, Mohamed A.; Ikram Burney

    2016-01-01

    Objectives: Marine organisms are a rich source of bioactive molecules with potential applications in medicine, biotechnology and industry; however, few bioactive compounds have been isolated from organisms inhabiting the Arabian Gulf and the Gulf of Oman. This study aimed to isolate and screen the anti-cancer activity of compounds and extracts from 40 natural products of marine organisms collected from the Gulf of Oman. Methods: This study was carried out between January 2012 and December ...

  12. The financial crisis: Caused by unpreventable or organized failures?

    OpenAIRE

    2009-01-01

    In this paper, I analyze cutaways of the current financial crisis against the background of normal accident theory, high reliability theory, and disaster incubation theory. To avoid future financial crises I recommend reducing pressures to make profit and organizing the global financial markets like high reliability organizations. Furthermore I argue that risk management within organizations must no longer only be a symbolic gesture. The paper's purpose is to break with the isolated financial...

  13. Cumulative effects of biochar, mineral and organic fertilizers on soil organic matter

    Science.gov (United States)

    Plaza, César; López-de-Sá, Esther G.; Gascó, Gabriel; Méndez, Ana; Zaccone, Claudio

    2016-04-01

    We investigated the effect of three consecutive annual applications of biochar at rates of 0 and 20 t ha-1, in a factorial combination with a mineral fertilizer (NPK and nitrosulfate) and two types of organic amendment (municipal solid waste compost and sewage sludge), on soil organic matter in a field experiment under Mediterranean conditions. Biochar increased significantly soil organic C content and C/N ratio. In biochar-amended soils, soil organic C increased significantly with the addition of municipal solid waste compost and sewage sludge. To capture organic matter protection mechanisms related to aggregation and mineral interaction, the soil samples will be fractionated into free (unprotected), intra-macroaggregate, intra-microaggregate, and mineral-associated organic matter pools, and the isolated fractions will be subjected to further chemical and spectroscopic analysis.

  14. PmrB Mutations Promote Polymyxin Resistance of Pseudomonas aeruginosa Isolated from Colistin-Treated Cystic Fibrosis Patients

    DEFF Research Database (Denmark)

    Moskowitz, Samuel M; Brannon, Mark K; Dasgupta, Nandini;

    2012-01-01

    of this organism (MICs of 8 to 64 mg/liter). To explore the role of PmrAB in high-level clinical polymyxin resistance, P. aeruginosa isolates from chronically colistin-treated cystic fibrosis patients, most with colistin MICs of >512 mg/liter, were analyzed. These cystic fibrosis isolates contained...

  15. High-Frequency Recovery of Quinupristin-Dalfopristin-Resistant Enterococcus faecium Isolates from the Poultry Production Environment

    OpenAIRE

    Hayes, Joshua R.; McIntosh, Angela C.; Qaiyumi, Sadaf; Johnson, Judith A.; English, Linda L.; Carr, Lewis E.; Wagner, David D.; Joseph, Sam W.

    2001-01-01

    The occurrence of resistance to the streptogramin quinupristin-dalfopristin in Enterococcus faecium isolates from chickens on the Eastern Seaboard, was evaluated. Quinupristin-dalfopristin resistance was found in 51 to 78% of E. faecium isolates from the food production environment. The high level of resistance in this organism suggests that this reservoir of resistance may compromise the therapeutic potential of quinupristin-dalfopristin.

  16. Vapor-barrier Vacuum Isolation System

    Science.gov (United States)

    Weinstein, Leonard M. (Inventor); Taminger, Karen M. (Inventor)

    2014-01-01

    A system includes a collimated beam source within a vacuum chamber, a condensable barrier gas, cooling material, a pump, and isolation chambers cooled by the cooling material to condense the barrier gas. Pressure levels of each isolation chamber are substantially greater than in the vacuum chamber. Coaxially-aligned orifices connect a working chamber, the isolation chambers, and the vacuum chamber. The pump evacuates uncondensed barrier gas. The barrier gas blocks entry of atmospheric vapor from the working chamber into the isolation chambers, and undergoes supersonic flow expansion upon entering each isolation chamber. A method includes connecting the isolation chambers to the vacuum chamber, directing vapor to a boundary with the working chamber, and supersonically expanding the vapor as it enters the isolation chambers via the orifices. The vapor condenses in each isolation chamber using the cooling material, and uncondensed vapor is pumped out of the isolation chambers via the pump.

  17. Study on three dimensional seismic isolation system

    International Nuclear Information System (INIS)

    Japan Nuclear Cycle Development Institute (JNC) and Japan Atomic Power Company (JAPC) launched joint research programs on structural design and three-dimensional seismic isolation technologies, as part of the supporting R and D activities for the feasibility studies on commercialized fast breeder reactor cycle systems. A research project by JAPC under the auspices of the Ministry of Economy, Trade, and Industry (METI) with technical support by JNC is included in this joint study. This report contains the results of the research on the three dimensional seismic isolation technology, and the results of this study are summarized in the following five aspects. (1) Study on Requirements for the Realization of 3-dimensional Base Isolation System. The simulated seismic wave 'case study S2', which was applied to the horizontal seismic isolation study for demonstrative FBR, was adopted applying to this study. Seismic response analyses of NPP building and R/V were conducted by this wave. The conditions required for the realization of 3-dimensional isolation system for FBR were clarified in terms of vertical frequency and damping ratio of the system, considering the design criteria for the core support plate etc. and their responses in acceleration and displacement. (2) Investigation and Clarification of 3-dimensional Isolation Concepts. Three types of seismic isolation system were examined on the feature of the isolation system, development status and problems to be solved. Then, six types of seismic isolation device were also investigated on the same aspects by literature. (3) Preliminary Evaluation of 3-dimensional Isolation Concepts. By preliminarily evaluating three types of seismic isolation system on easiness, effectiveness and cost of development, 3-dimensional entire building base isolation system and horizontal entire building base isolation system with vertically isolated for main equipments were selected for FBR. Then, air and dish springs were selected as

  18. Methanogenic Archaea Isolated from Taiwan's Chelungpu Fault▿ †

    Science.gov (United States)

    Wu, Sue-Yao; Lai, Mei-Chin

    2011-01-01

    Terrestrial rocks, petroleum reservoirs, faults, coal seams, and subseafloor gas hydrates contain an abundance of diverse methanoarchaea. However, reports on the isolation, purification, and characterization of methanoarchaea in the subsurface environment are rare. Currently, no studies investigating methanoarchaea within fault environments exist. In this report, we succeeded in obtaining two new methanogen isolates, St545MbT of newly proposed species Methanolobus chelungpuianus and Methanobacterium palustre FG694aF, from the Chelungpu fault, which is the fault that caused a devastating earthquake in central Taiwan in 1999. Strain FG694aF was isolated from a fault gouge sample obtained at 694 m below land surface (mbls) and is an autotrophic, mesophilic, nonmotile, thin, filamentous-rod-shaped organism capable of using H2-CO2 and formate as substrates for methanogenesis. The morphological, biochemical, and physiological characteristics and 16S rRNA gene sequence analysis revealed that this isolate belongs to Methanobacterium palustre. The mesophilic strain St545MbT, isolated from a sandstone sample at 545 mbls, is a nonmotile, irregular, coccoid organism that uses methanol and trimethylamine as substrates for methanogenesis. The 16S rRNA gene sequence of strain St545MbT was 99.0% similar to that of Methanolobus psychrophilus strain R15 and was 96 to 97.5% similar to the those of other Methanolobus species. However, the optimal growth temperature and total cell protein profile of strain St545MbT were different from those of M. psychrophilus strain R15, and whole-genome DNA-DNA hybridization revealed less than 20% relatedness between these two strains. On the basis of these observations, we propose that strain St545MbT (DSM 19953T; BCRC AR10030; JCM 15159) be named Methanolobus chelungpuianus sp. nov. Moreover, the environmental DNA database survey indicates that both Methanolobus chelungpuianus and Methanobacterium palustre are widespread in the subsurface

  19. Antibiogram study of aerobic bacterial isolates from uropathogens

    Directory of Open Access Journals (Sweden)

    Mallikarjuna Reddy C, Himabindu M, Maity Soumendranath, Kanta RC, Kapur Indu

    2014-04-01

    Full Text Available Background: Bacteria are capable of invading and infecting humans, leading to disease and sometimes death. Systems and tissues in human body are vulnerable to different organisms. Infection pattern is likely to differ by geographical regions. Aim: This study was aimed to isolate and identify the type of aerobic bacteria causing Urinary Tract Infections (UTI in different age groups and sexes, and also in some predisposing conditions. Their antibiogram also was done. Materials and Methods: Midstream urine sample collected aseptically from 276 patients were subjected for isolation and identification of aerobic bacteria by standard technique and subsequently antibiogram was done by Kirby –Bayer Method. Both sexes of patients with an age range of 10-70 years and patients with diabetes (22, hypertension (8 and anemia (8 were also included in the study. Results: Escherichia coli was the predominant organism(50% among other isolates – Klebsiella species (27.3%, Proteus species(7.14%, Staphylococcus saprophyticus (5.95%, Staphylococcus aureus (3.57%, Enterococci (3.57%, Pseudomonas species(2.38%. UTI was more common among patients of 60 and more years of age; however, incidence was more in female patients (36.2 – 38.5% compared with male patients (25-30%. Anemia, Diabetes and Hypertension conditions were found to predispose UTI. Aminoglycosides and Quinolones were found to be more effective against the isolates. Conclusion: The present study reveals in spite of the topographical diversity, the infecting bacterial isolates from this area were found to be the same as from any other part of India.

  20. The Pathogenomic Sequence Analysis of B. cereus and B. Thuringiensis isolates closely related to Bacillus anthracis

    Energy Technology Data Exchange (ETDEWEB)

    Han, C S; Xie, G; Challacombe, J F; Altherr, M R; Bhotika, S S; Bruce, D; Campbell, C S; Campbell, M L; Chen, J; Chertkov, O; Cleland, C; Dimitrijevic-Bussod, M; Doggett, N A; Fawcett, J J; Glavina, T; Goodwin, L A; Hill, K K; Hitchcock, P; Jackson, P J; Keim, P; Kewalramani, A R; Longmire, J; Lucas, S; Malfatti, S; McMurry, K; Meincke, L J; Misra, M; Moseman, B L; Mundt, M; Munk, A C; Okinaka, R T; Parson-Quintana, B; Reilly, L P; Richardson, P; Robinson, D L; Rubin, E; Saunders, E; Tapia, R; Tesmer, J G; Thayer, N; Thompson, L S; Tice, H; Ticknor, L O; Wills, P L; Gilna, P; Brettin, T S

    2005-10-12

    The sequencing and analysis of two close relatives of Bacillus anthracis are reported. AFLP analysis of over 300 isolates of B. cereus, B. thuringiensis and B. anthracis identified two isolates as being very closely related to B. anthracis. One, a B. cereus, BcE33L, was isolated from a zebra carcass in Nambia; the second, a B. thuringiensis, 97-27, was isolated from a necrotic human wound. The B. cereus appears to be the closest anthracis relative sequenced to date. A core genome of over 3,900 genes was compiled for the Bacillus cereus group, including B anthracis. Comparative analysis of these two genomes with other members of the B. cereus group provides insight into the evolutionary relationships among these organisms. Evidence is presented that differential regulation modulates virulence, rather than simple acquisition of virulence factors. These genome sequences provide insight into the molecular mechanisms contributing to the host range and virulence of this group of organisms.

  1. Antimicrobial resistance and virulence profiles of Salmonella isolated from butcher shops in Minas Gerais, Brazil.

    Science.gov (United States)

    Cossi, Marcus Vinícius Coutinho; Burin, Raquel Cristina Konrad; Lopes, Danilo Augusto; Dias, Mariane Rezende; Castilho, Natalia Parma Augusto de; de Arruda Pinto, Paulo Sérgiode; Nero, Luís Augusto

    2013-09-01

    Salmonella can contaminate finished products of butcher shops, mainly through cross-contamination of utensils exposed to raw materials. To identify the main sources of contamination with this foodborne pathogen in four butcher shop environments, surface samples were obtained from employees' hands, cutting boards, knives, floor of the refrigeration room, meat grinders, and meat tenderizers (32 samples per area) and analyzed for Salmonella using the International Organization for Standardization method 6579, with modifications. Suspect isolates were identified by PCR (targeting ompC), and confirmed Salmonella isolates were subjected to pulsed-field gel electrophoresis (after treatment with restriction enzyme XbaI), analyzed for the presence of virulence genes (invA, sefA, and spvC), and screened for resistance to 12 antimicrobials. Salmonella isolates was identified only on cutting boards (five samples) from three butcher shops. Fifteen isolates were confirmed as Salmonella belonging to four pulse types (similarity of 71.1 to 100%). The invA gene was detected in 13 isolates, and the sefA was found in 8 isolates; no isolate carried spvC. All tested isolates were resistant to clindamycin and sensitive to amikacin and cefotaxine, and all isolates were resistant to at least 3 of the 12 antimicrobials tested. The results indicate the importance of cutting boards as a source of Salmonella contamination in butcher shops. The presence of multidrug-resistant Salmonella strains possessing virulence genes highlights the health risks for consumers. PMID:23992511

  2. Deinococcus antarcticus sp. nov., isolated from soil.

    Science.gov (United States)

    Dong, Ning; Li, Hui-Rong; Yuan, Meng; Zhang, Xiao-Hua; Yu, Yong

    2015-02-01

    A pink-pigmented, non-motile, coccoid bacterial strain, designated G3-6-20(T), was isolated from a soil sample collected in the Grove Mountains, East Antarctica. This strain was resistant to UV irradiation (810 J m(-2)) and slightly more sensitive to desiccation as compared with Deinococcus radiodurans. Phylogenetic analyses based on the 16S rRNA gene sequence of the isolate indicated that the organism belongs to the genus Deinococcus. Highest sequence similarities were with Deinococcus ficus CC-FR2-10(T) (93.5 %), Deinococcus xinjiangensis X-82(T) (92.8 %), Deinococcus indicus Wt/1a(T) (92.5 %), Deinococcus daejeonensis MJ27(T) (92.3 %), Deinococcus wulumuqiensis R-12(T) (92.3 %), Deinococcus aquaticus PB314(T) (92.2 %) and Deinococcus radiodurans DSM 20539(T) (92.2 %). Major fatty acids were C18 : 1ω7c, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), anteiso-C15 : 0 and C16 : 0. The G+C content of the genomic DNA of strain G3-6-20(T) was 63.1 mol%. Menaquinone 8 (MK-8) was the predominant respiratory quinone. Based on its phylogenetic position, and chemotaxonomic and phenotypic characteristics, strain G3-6-20(T) represents a novel species of the genus Deinococcus, for which the name Deinococcus antarcticus sp. nov. is proposed. The type strain is G3-6-20(T) ( = DSM 27864(T) = CCTCC AB 2013263(T)). PMID:25351880

  3. Characterization of Erysipelothrix rhusiopathiae isolates from poultry, pigs, emus, the poultry red mite and other animals

    OpenAIRE

    2009-01-01

    Abstract Erysipelothrix rhusiopathiae is the causative agent of erysipelas in mammals and birds, especially pigs and poultry. In order to investigate the suitability of different subtyping methods for genetic and phenotypic similarities among Swedish isolates of the organism, 45 isolates from poultry (n=23), pigs (n=17), emus (n=2) and the poultry red mite Dermanyssus gallinae (n=3) were investigated by serotyping, pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptib...

  4. Isolation of Acanthamoeba-Specific Antibodies from a Bacteriophage Display Library

    OpenAIRE

    Khan, Naveed A.; Greenman, John; Topping, Katherine P.; Victoria C. Hough; Temple, Graham S.; Paget, Timothy A.

    2000-01-01

    Acanthamoeba causes opportunistic eye infections in humans, which can lead to severe keratitis and may ultimately result in blindness. Current methods for identifying this organism rely on culture and microscopy. In this paper, we describe the isolation of antibody fragments that can be used for the unequivocal identification of Acanthamoeba. A bacteriophage antibody display library was used to isolate antibody fragments that bind specifically to Acanthamoeba. Individual clones were studied b...

  5. Data on the isolation of immunoglobulin from the serum of the green frog: Rana esculenta

    OpenAIRE

    Stošić Svetlana; Divjak Isidora; Ješić Snežana; Nešić V.; Račić A.; Popović Nada

    2006-01-01

    Even though the green frog (Rana esculenta) is often used as an experimental model for further studies of physiological laws, little is still known about its serum proteins and their role in immunity. Its serum proteins have been studied quite extensively, as when they are taken up into the organism of another animal they represent antigens themselves. In this work, an attempt was made to isolate some frog serum proteins and to investigate the electrophoretic qualities of the isolated compone...

  6. PRIMARY AND SECONDARY IMMUNE RESPONSE TO FORMALIN INACTIVATED STREPTOCOCCUS AGALACTIAE ISOLATES IN RABBITS

    OpenAIRE

    M. ABUBAKAR, G. MUHAMMAD1 AND K. IBRAHIM

    2006-01-01

    The present study was conducted to evaluate primary and secondary immune response to formalin inactivated Streptococcus agalactiae isolates in rabbits. Streptococcus agalactiae was isolated from mastitic buffaloes. The organism was characterized on the basis of morphological, cultural and biochemical tests. An increased geomean antibody titer was observed in rabbits inoculated with single dose (Group A) and double dose (Group B) of Streptococcus agalactiae antigen. It was also evident from th...

  7. Isolation and Characterization of Cellulose-decomposing Bacteria Inhabiting Sawdust and Coffee Residue Composts

    OpenAIRE

    Eida, Mohamed Fathallh; Nagaoka, Toshinori; Wasaki, Jun; Kouno, Kenji

    2012-01-01

    Clarifying the identity and enzymatic activities of microorganisms associated with the decomposition of organic materials is expected to contribute to the evaluation and improvement of composting processes. In this study, we examined the cellulolytic and hemicellulolytic abilities of bacteria isolated from sawdust compost (SDC) and coffee residue compost (CRC). Cellulolytic bacteria were isolated using Dubos mineral salt agar containing azurine cross-linked (AZCL) HE-cellulose. Bacterial iden...

  8. Reversible Pulmonary Hypertension and Isolated Right-sided Heart Failure Associated with Hyperthyroidism

    OpenAIRE

    Ismail, Hassan M.

    2007-01-01

    Hyperthyroidism may present with signs and symptoms related to dysfunction of a variety of organs. Cardiovascular pathology in hyperthyroidism is common. A few case reports describe isolated right heart failure, tricuspid regurgitation, and pulmonary hypertension as the prominent cardiovascular manifestations of hyperthyroidism. Although most textbooks do not mention hyperthyroidism as a cause of pulmonary hypertension and isolated right heart failure, the literature suggests that some hypert...

  9. STREPTOCOCCUS PHOCAE ISOLATED FROM A SPOTTED SEAL (PHOCA LARGHA) WITH PYOMETRA IN ALASKA

    OpenAIRE

    Hueffer, Karsten; Lieske, Camilla L; McGilvary, Lisa M.; Hare, Rebekah F.; Debra L Miller; O’Hara, Todd M.

    2011-01-01

    A spotted seal harvested by subsistence hunters in Kotzebue Sound, Alaska (USA), showed a grossly enlarged uterus and associated lymph nodes. Streptococcus phocae was isolated from the purulent uterine discharge. Histopathologic examination revealed inflammation that was limited to the uterine mucosa. Lymph nodes draining the affected organ were reactive but no evidence of active infection was found in the lymph nodes. This report is the first Streptococcus phocae isolated from spotted seals ...

  10. DNA Hybridization of Escherichia coli Strains Isolated from Uteri and Fecal Samples of Bitches with Pyometra

    OpenAIRE

    SANCAK, Aziz Arda

    2004-01-01

    Escherichia coli is the most common bacterium that has been isolated from the bacterial culture of uterine and fecal samples of dogs with pyometra. The aim of the present study was to determine whether this organism could be relevant to the pathogenesis of pyometra in dogs. Fecal and uterine samples were collected from 17 bitches with pyometra. E. coli strains were isolated in all samples. Representative colonies of E. coli from each sample were analyzed for pathogenicity determinants by h...

  11. ACTINOMYCES NAESLUNDII ISOLATED FROM BONE MARROW BIOPSIES OBTAINED FROM PATIENTS WITH MALIGNANT LYMPHORETICULAR DISEASES

    Directory of Open Access Journals (Sweden)

    I.Mobedi

    1981-08-01

    Full Text Available Different types of Actinomyces spp isolated from the oral cavity of human in healthy as well as in pathological state. Internally it is only found in association with pathological organs. Recently Actinomyces naeslundii has been isolated from blood culture of a leukemic patient. Present studies indicate the association of this microorganism with cells obtained from bone marrow by punction in more than 40 percent of patients of patients with malignant lymphoreticular disease.

  12. ACTINOMYCES NAESLUNDII ISOLATED FROM BONE MARROW BIOPSIES OBTAINED FROM PATIENTS WITH MALIGNANT LYMPHORETICULAR DISEASES

    OpenAIRE

    Mobedi, I; J.Vandyoussefi; H.Rassa; HA.Fardin

    1981-01-01

    Different types of Actinomyces spp isolated from the oral cavity of human in healthy as well as in pathological state. Internally it is only found in association with pathological organs. Recently Actinomyces naeslundii has been isolated from blood culture of a leukemic patient. Present studies indicate the association of this microorganism with cells obtained from bone marrow by punction in more than 40 percent of patients of patients with malignant lymphoreticular disease.

  13. The isolation, enumeration, and characterization of Rhizobium bacteria of the soil in Wamena Biological Garden

    Directory of Open Access Journals (Sweden)

    SRI PURWANINGSIH

    2005-04-01

    Full Text Available The eleven soil samples have been isolated and characterized. The aims of the study were to get the pure culture and some data which described about enumeration and especially their characters in relation to the acids and bases reaction in their growth. The isolation of the bacteria use Yeast Extract Mannitol Agar medium (YEMA while the characterization by using YEMA medium mixed with Brom Thymol Blue and Congo Red indicators respectively. The results showed that eighteen isolates have been isolated which consisted of three low growing and fifteen fast growing bacteria. Two isolates were not indicated Rhizobium and sixteen were Rhizobium. Density of Rhizobium enumeration was varied which related to soil organic matter content. The enumeration bacteria in YEMA medium were in the range of 0.6 x 105 and 11.6 x 105 CFU /g soil. The highest population was found in soil sample of Wieb vegetation.

  14. Isolation and identification of Helicobacter spp, from canine and feline gastric mucosa

    DEFF Research Database (Denmark)

    Jalava, K.; On, Stephen L.W.; VanDamme, P.A.R.;

    1998-01-01

    It is known that virtually all healthy adult dogs and cats harbor spiral helicobacters in their gastric mucosa, Three species, Helicobacter felis, Helicobacter bizzozeronii, and Helicobacter salomonis have been isolated in vitro from the gastric mucosa of these animals. The aims of this study were...... standardized conventional phenotypic tests, whole-cell protein profiling, and ultrastructural analysis in identifying the different species isolated from canine and feline gastric mucose. We cultured 95 and 22 gastric mucosal biopsies from dogs and cats, respectively. Twenty-one H. bizzozeronii strains, 8 H....... felis strains, 8 H. salomonis strains, 3 mixed cultures, 2 "Flexispira rappini"-like organisms, and 3 as get uncharacterized strains were isolated from the dogs, and 3 H. felis strains were isolated from the cats. The methods used here yielded Helicobacter isolation rates of 51% from dogs and 13.6% from...

  15. Next-generation sequencing of Coccidioides immitis isolated during cluster investigation.

    Science.gov (United States)

    Engelthaler, David M; Chiller, Tom; Schupp, James A; Colvin, Joshua; Beckstrom-Sternberg, Stephen M; Driebe, Elizabeth M; Moses, Tracy; Tembe, Waibhav; Sinari, Shripad; Beckstrom-Sternberg, James S; Christoforides, Alexis; Pearson, John V; Carpten, John; Keim, Paul; Peterson, Ashley; Terashita, Dawn; Balajee, S Arunmozhi

    2011-02-01

    Next-generation sequencing enables use of whole-genome sequence typing (WGST) as a viable and discriminatory tool for genotyping and molecular epidemiologic analysis. We used WGST to confirm the linkage of a cluster of Coccidioides immitis isolates from 3 patients who received organ transplants from a single donor who later had positive test results for coccidioidomycosis. Isolates from the 3 patients were nearly genetically identical (a total of 3 single-nucleotide polymorphisms identified among them), thereby demonstrating direct descent of the 3 isolates from an original isolate. We used WGST to demonstrate the genotypic relatedness of C. immitis isolates that were also epidemiologically linked. Thus, WGST offers unique benefits to public health for investigation of clusters considered to be linked to a single source. PMID:21291593

  16. Preparation of isolated carbon nanotubes

    International Nuclear Information System (INIS)

    Full text: Carbon nanotubes are of great interest for a large range of applications from physical chemistry, solid state physics to molecular quantum optics. We propose the preparation of molecular beams of isolated carbon nanotubes for future matter wave experiments, as well as for applications in the material sciences and spectroscopy. Carbon nanotubes may be particularly interesting for quantum experiments because of their low ionization threshold, high mechanical stability and high polarizability. This is expected to facilitate the cooling, coherent manipulation and efficient detection of such molecular beams. For this purpose we are investigating different methods of solvation, isolation and shortening of carbon nanotubes from commercial bundles. Length and diameter distributions are recorded by SPM whereas the unbundling of the tubes is determined by absorption spectroscopy. Established methods from physical chemistry, such as laser desorption are currently being modified and studied as potential tools for generating beams of nanotubes in the mass range of around 50.000-100.000 amu. (author)

  17. Ordered organic-organic multilayer growth

    Energy Technology Data Exchange (ETDEWEB)

    Forrest, Stephen R.; Lunt, Richard R.

    2016-04-05

    An ordered multilayer crystalline organic thin film structure is formed by depositing at least two layers of thin film crystalline organic materials successively wherein the at least two thin film layers are selected to have their surface energies within .+-.50% of each other, and preferably within .+-.15% of each other, whereby every thin film layer within the multilayer crystalline organic thin film structure exhibit a quasi-epitaxial relationship with the adjacent crystalline organic thin film.

  18. Protein isolation using affinity chromatography

    OpenAIRE

    Besselink, T.

    2012-01-01

    Many product or even waste streams in the food industry contain components that may have potential for e.g. functional foods. These streams are typically large in volume and the components of interest are only present at low concentrations. A robust and highly selective separation process should be developed for efficient isolation of the components. Affinity chromatography is such a selective method. Ligands immobilized to a stationary phase (e.g., a resin or membrane) are used to bind the c...

  19. Isolated tuberculosis of the sternum

    International Nuclear Information System (INIS)

    Isolated tuberculous foci of the sternum are rare. In a period of 26 years (1955 to 1981) we have treated ten sternal lesions surgically in our clinic. Eight of these were tuberculous. The clinical material (three females, five males) is described, including diagnostic and therapeutic details. The frequency, localisation, clinical features, diagnosis, treatment, prognosis and differential diagnosis of specific sternal infections are discussed and the relevant literature is reviewed. (orig.)

  20. Multipole moments of isolated horizons

    International Nuclear Information System (INIS)

    To every axi-symmetric isolated horizon we associate two sets of numbers, Mn and Jn with n = 0, 1, 2, ..., representing its mass and angular momentum multipoles. They provide a diffeomorphism invariant characterization of the horizon geometry. Physically, they can be thought of as the 'source multipoles' of black holes in equilibrium. These structures have a variety of potential applications ranging from equations of motion of black holes and numerical relativity to quantum gravity

  1. Customizable Isolation in Transactional Workflow

    OpenAIRE

    Guabtni, Adnene; Charoy, François; Godart, Claude

    2005-01-01

    In Workflow Management Systems (WFMSs) safety of execution is a main need of more and more business processes and transactional workflows are real needs inside enterprizes. In previous works, transactional models consider mainly atomicity as the main issue regarding long term transactions. It rarely consider the fact that many processes may run concurrently and thus access and update the same data. Usually, the main isolation item is the data on which we apply locking approaches and this atti...

  2. Isolation and Aggregation in Economics

    OpenAIRE

    Schlicht, Ekkehart

    1985-01-01

    In explaining economic phenomena, economic analysis concentrates on selected influences and fixes the host of other factors under a ceteris paribus clause. This view, which goes back to Alfred Marshall (1842-1924), is developed in the first part of the book. Aggregation is viewed as a particular application of ceteris paribus analysis - isolation from "structural effects". This leads to an approach, called "closed aggregation", which was introduced by Kenneth May and is also implicit in Keyne...

  3. Fungi isolated in school buildings

    OpenAIRE

    Elżbieta Ejdys

    2013-01-01

    The aim of the study was to determine the species composition of fungi occurring on wall surfaces and in the air in school buildings. Fungi isolated from the air using the sedimentation method and from the walls using the surface swab technique constituted the study material. Types of finish materials on wall surfaces were identified and used in the analysis. Samples were collected in selected areas in two schools: classrooms, corridors, men's toilets and women's toilets, cloakrooms, sports c...

  4. Autosomal dominant isolated ('uncomplicated') microcephaly.

    OpenAIRE

    Merlob, P; Steier, D; Reisner, S H

    1988-01-01

    A large family (13 affected members in three generations) is reported in which isolated microcephaly occurred without any other dysmorphic or neurological abnormalities. The family pedigree confirms the autosomal dominant mode of inheritance with incomplete penetrance, including one example of male to male transmission and the occurrence of a non-manifesting heterozygote resulting in a 'skipped generation'. There is considerable variation in the phenotypic expression of autosomal dominant mic...

  5. Multilocus sequence typing of Candida albicans isolates from a burn intensive care unit in Iran.

    Science.gov (United States)

    Afsarian, Mohammad H; Badali, Hamid; Boekhout, Teun; Shokohi, Tahereh; Katiraee, Farzad

    2015-03-01

    Burn intensive care unit (BICU) patients are specifically exposed to deep-seated nosocomial infections due to Candida albicans. Superficial carriage of C. albicans is a potential source of infection and dissemination, and typing methods could be useful to trace the different isolates. Multilocus sequence typing is a powerful genotyping method for pathogenic micro-organisms, including Candida albicans. Thirty clinical isolates of C. albicans obtained from 22 patients that were admitted to the BICU from a burn hospital at Sari, Mazandaran state, Iran, were studied epidemiologically by multilocus sequence typing (MLST). Seventy-five variable nucleotide sites were found. Sixty-two alleles were identified among the seven loci of the C. albicans isolates and one new allele was obtained. Eighteen diploid sequence types (DSTs) were identified, and among those 10 were new. These isolates belonged to nine clonal clusters (CCs) while two isolates occurred as singletons. Eleven (36.7 %) isolates belonged to CC 124 after eBURST analysis and 13 isolates (43.3 %) were assigned to clade 4. Approximately 17 % of the 30 isolates belonged to clade 1 (CC 69 and CC 766). Isolates from several patients with burns were found to be related genetically. Some patients yielded multiple isolates with identical DSTs, suggesting colonization or infection caused by cross-contamination between patients. Isolates that show identical or similar allelic profiles are presumed to be identical or closely related and may be used to evaluate the genetic relationships between isolates from a specific environment such as the BICU. PMID:25596113

  6. Pancreatic Islets: Methods for Isolation and Purification of Juvenile and Adult Pig Islets.

    Science.gov (United States)

    Brandhorst, Heide; Johnson, Paul R V; Brandhorst, Daniel

    2016-01-01

    The current situation of organ transplantation is mainly determined by the disbalance between the number of available organs and the number of patients on the waiting list. This obvious dilemma might be solved by the transplantation of porcine organs into human patients. The metabolic similarities which exist between both species made pancreatic islets of Langerhans to that donor tissue which will be most likely transplanted in human recipients. Nevertheless, the successful isolation of significant yields of viable porcine islets is extremely difficult and requires extensive experiences in the field. This review is focussing on the technical challenges, pitfalls and particularities that are associated with the isolation of islets from juvenile and adult pigs considering donor variables that can affect porcine islet isolation outcome. PMID:27586421

  7. Soil organic matter studies

    International Nuclear Information System (INIS)

    The use of isotopes in soil organic matter studies is reviewed. Such studies include measuring the input of organic carbon into soils, the decomposition of isotope-labelled materials such as organic compounds and microbial and plant material in soils, the characterization of soil organic matter and the availability of nutrients released from organic residues. (U.K.)

  8. Isolation and identification of species of Alicyclobacillus from orchard soil in the Western Cape, South Africa.

    Science.gov (United States)

    Groenewald, Willem H; Gouws, Pieter A; Witthuhn, R Corli

    2008-01-01

    Alicyclobacilli were isolated from orchard soil collected from an apple and pear farm in Elgin, Western Cape, South Africa. Morphological, biochemical and physiological characteristics of the isolates were used to presumptively classify them as belonging to the genus Alicyclobacillus. Strains were identified to species level by polymerase chain reaction (PCR) with genus-specific primers, and 16S ribosomal RNA (rRNA) gene sequencing. To our knowledge this is the first report on the isolation of Alicyclobacillus acidoterrestris and Alicyclobacillus acidocaldarius from orchard soil. The presence of these organisms in the soil suggests a possible source of contamination for the final fruit juice, concentrate or pulp. PMID:17938854

  9. Helicobacter ganmani sp nov., a urease-negative anaerobe isolated from the intestines of laboratory mice

    DEFF Research Database (Denmark)

    Robertson, B.R.; O'Rourke, J.L.; Vandamme, P.;

    2001-01-01

    Spiral bacteria were isolated from the intestines of laboratory mice during a study examining the presence of Helicobacter species and other spiral organisms naturally infecting mice maintained at four different animal facilities in Sydney, Australia. One group of 17 isolates, cultured from mice...... from three of the four facilities, were found to be helicobacters but did not fall within any of the 18 currently recognized species. These isolates were unusual in that they only grew anaerobically at 37 degreesC and were incapable of growth under microaerobic conditions. Like Helicobacter rodentium...

  10. First Isolation of a Giant Virus from Wild Hirudo medicinalis Leech: Mimiviridae isolation in Hirudo medicinalis

    Directory of Open Access Journals (Sweden)

    Mondher Boughalmi

    2013-11-01

    Full Text Available Giant viruses and amoebae are common in freshwater, where they can coexist with other living multicellular organisms. We screened leeches from the species Hirudo medicinalis for giant viruses. We analyzed five H. medicinalis obtained from Tunisia (3 and France (2. The leeches were decontaminated and then dissected to remove internal parts for co-culture with Acanthamoeba polyphaga. The genomes of isolated viruses were sequenced on a 454 Roche instrument, and a comparative genomics analysis was performed. One Mimivirus was isolated and the strain was named Hirudovirus. The genome assembly generated two scaffolds, which were 1,155,382 and 25,660 base pairs in length. Functional annotations were identified for 47% of the genes, which corresponds to 466 proteins. The presence of Mimividae in the same ecological niche as wild Hirudo may explain the presence of the mimivirus in the digestive tract of the leech, and several studies have already shown that viruses can persist in the digestive tracts of leeches fed contaminated blood. As leeches can be used medically and Mimiviruses have the potential to be an infectious agent in humans, patients treated with leeches should be surveyed to investigate a possible connection.

  11. Isolation of haloorganic groundwater humic substances

    DEFF Research Database (Denmark)

    Krog, M.; Grøn, C.

    1995-01-01

    Humic substances were isolated from groundwater according to a revised method designed to avoid organohalogen artefacts. The prepared humic substances exhibited lower halogen contents than humic substances isolated according to the conventionally used method. Excessive oxidation or hydrolysis...

  12. Mycoplasma contamination of Chlamydia pneumoniae isolates

    DEFF Research Database (Denmark)

    Huniche, BS; Jensen, Lise Torp; Birkelund, Svend;

    1998-01-01

    We examined 6 C. pneumonia isolates from The American Type Culture Collection (ATCC) and 2 Finnish isolates for Mycoplasma contamination. Three of the ATCC isolates and both of the Finnish isolates were Mycoplasma-contaminated. The contaminants were characterized by means of growth in BEa and BEg...... media, immunoblotting, polymerase chain reaction and pulsed field gel electrophoresis. Two of the 6 ATCC isolates [ATCC VR1355 (TWAR strain 2043) and ATCC VR1356 (TWAR strain 2023)] were infected with Mycoplasma hominis and 1 isolate [ATCC VR2282 (TWAR strain TW183)] was contaminated with both...... Mycoplasma hominis and Mycoplasma orale, whereas 3 of the ATCC isolates [ATCC VR1310, ATCC VR1360 (TWAR strain CM-1) and ATCC 53592 (TWAR strain AR39)] were not contaminated. The Finnish C. pneumoniae isolates Kajaani 6 and Parola were found to be contaminated with M. hominis and M. orale, respectively...

  13. Genetics Home Reference: isolated hyperCKemia

    Science.gov (United States)

    ... the elevated blood levels of creatine kinase that characterize this condition. In addition to isolated hyperCKemia , CAV3 ... cause the disorder. In most cases, an affected person has one parent with isolated hyperCKemia or another ...

  14. Isolation of Cellulolytic Actinomycetes from Marine Sediments

    OpenAIRE

    Veiga, Manuel; Esparis, Azucena; Fabregas, Jaime

    1983-01-01

    The cellulolytic activity of 36 actinomycetes strains isolated from marine sediments was investigated by the cellulose-azure method. Approximately 50% of the isolates exhibited various degrees of cellulolytic activity.

  15. Isolated malignant melanoma metastasis to the pancreas

    DEFF Research Database (Denmark)

    Larsen, Anne K; Krag, Christen; Geertsen, Poul;

    2013-01-01

    SUMMARY: Malignant melanomas rarely develop isolated pancreatic metastases. We describe a unique patient who is still alive 22 years following an isolated pancreatic melanoma metastasis, and we review the sparse literature in the field....

  16. Isolation of haloorganic groundwater humic substances

    DEFF Research Database (Denmark)

    Krog, M.; Grøn, C.

    Humic substances were isolated from groundwater according to a revised method designed to avoid organohalogen artefacts. The prepared humic substances exhibited lower halogen contents than humic substances isolated according to the conventionally used method. Excessive oxidation or hydrolysis was...

  17. Coaggregation occurs between microorganisms isolated from different environments.

    Science.gov (United States)

    Stevens, Michael R E; Luo, Ting L; Vornhagen, Jay; Jakubovics, Nicholas S; Gilsdorf, Janet R; Marrs, Carl F; Møretrø, Trond; Rickard, Alexander H

    2015-11-01

    Coaggregation, the specific recognition and adherence of different microbial species, is thought to enhance biofilm formation. To date, no studies have focused on the ability of microorganisms isolated from a broad range of environments to coaggregate with each other and it is unclear whether coaggregation promotes the transmission of microorganisms between environmental niches. We aimed to evaluate the coaggregation ability of 29 bacteria and one fungus, isolated from a range of different environments, and to characterize the cell-surface polymers that mediate coaggregation between selected pairs. Strains were categorized as belonging to one of the four microbial archetypes: aquatic, broad environment, human opportunistic pathogen or human oral. A total of 23 of the 30 strains (77%) coaggregated with at least one other and 21/30 (70%) coaggregated with strains belonging to other archetypes. Nasopharyngeal bacteria belonging to the human opportunistic pathogen archetype showed the least number of coaggregations, and five Haemophilus influenzae strains did not coaggregate. Protease and sugar treatments indicated that coaggregation between strains of different archetypes was often mediated by lectin-saccharide interactions (9 of 15 evaluated pairs). In conclusion, coaggregation can occur between taxonomically disparate species isolated from discrete environments. We propose that these organisms be labeled as 'cross-environment coaggregating organisms'. The ability to coaggregate may aid species to colonize non-indigenous biofilms. PMID:26475462

  18. Toxins and genes isolated from scorpions of the genus Tityus.

    Science.gov (United States)

    Becerril, B; Marangoni, S; Possani, L D

    1997-06-01

    Scorpion venoms contain a variety of low mol. wt peptides toxic to different organisms. These peptides have been intensively studied because they represent excellent models for investigating structure-function relationships and they are also fine probes for studying ionic channel functions. This review deals with the biological and chemical aspects of toxic peptides that affect Na+ or K+ channels and the cloning of the cDNAs and genes encoding the main alpha and beta neurotoxins present in the venom of the three most dangerous species of Brazilian scorpion, Tityus bahiensis, Tityus stigmurus and Tityus serrulatus, and the Venezuelan scorpion Tityus discrepans. At least 16 different peptides specific for Na+ channels and five affecting K+ channels were isolated and characterized from the venom of these scorpions. The isolation of cDNAs and genes encoding four distinct toxins has permitted the elucidation of their nucleotide sequences as well as their genomic organization. Venoms and isolated toxins from scorpions of the genus Tityus were shown to enhance the secretory activity of the pancreas. Antisera obtained against venom of T. serrulatus show cross-reactivity with other species of the Brazilian scorpions. PMID:9241777

  19. Degradation of transuranic-contaminated wastes under geological isolation conditions

    International Nuclear Information System (INIS)

    Results of extensive experimental studies on the degradation of existing defense transuranic-contaminated (TRU) wastes are presented. Environmental conditions for waste degradation are representative of deep geological isolation of TRU wastes in a bedded-salt repository, as anticipated for the proposed Waste Isolation Pilot Plant in southeastern New Mexico. TRU-contaminated waste matrices investigated include cellulosics, plastics, rubbers, mixed organic composite, bitumen, a developmental concrete-TRU ash matrix, mild steel, and inorganic process sludges. Degradation mechanisms studied were alpha radiolysis, microbial action, thermal decomposition and dewatering, and chemical corrosion. Experimental variables are waste composition, alpha-contamination level, temperature, pressure, time, water or brine content, aerobic or anaerobic conditions, etc. Results of waste degradation are presented in terms of gas generation rates, gas compositions, container corrosion rates, effectiveness of anti-corrosion coatings, microbial formation and degradation of chelating agents. Based on experimental results, the relative quantities of gas generated by individual mechanisms acting on the organic matrix wastes can be ranked as follows: (1) microbial action, (2) thermal decomposition (at 400C and higher), (3) alpha radiolysis, and (4) corrosion. Corrosion of metallic waste canisters and realistic bedded-salt repository conditions was determined not to have a significant effect on potential waste retrievability. The technical data provided in these studies are used in assessments to determine whether existing forms of TRU wastes are acceptable for safe geological isolation. (author)

  20. Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation.

    Science.gov (United States)

    Amend, Sarah R; Valkenburg, Kenneth C; Pienta, Kenneth J

    2016-01-01

    Investigation of the bone and the bone marrow is critical in many research fields including basic bone biology, immunology, hematology, cancer metastasis, biomechanics, and stem cell biology. Despite the importance of the bone in healthy and pathologic states, however, it is a largely under-researched organ due to lack of specialized knowledge of bone dissection and bone marrow isolation. Mice are a common model organism to study effects on bone and bone marrow, necessitating a standardized and efficient method for long bone dissection and bone marrow isolation for processing of large experimental cohorts. We describe a straightforward dissection procedure for the removal of the femur and tibia that is suitable for downstream applications, including but not limited to histomorphologic analysis and strength testing. In addition, we outline a rapid procedure for isolation of bone marrow from the long bones via centrifugation with limited handling time, ideal for cell sorting, primary cell culture, or DNA, RNA, and protein extraction. The protocol is streamlined for rapid processing of samples to limit experimental error, and is standardized to minimize user-to-user variability. PMID:27168390

  1. Serotype and serovar distribution of Neisseria gonorrhoeae isolated from high-risk populations in Bangladesh.

    Science.gov (United States)

    Alam, M A; Chowdhury, M Z; Ahmed, F; Alam, A; Hossain, M A

    2012-12-01

    Neisseria gonorrhoeae, the causative agent of gonococcal infection, is known to frequently change their characteristics to evade host immune mechanism. Characterization of the clinical isolates of the organism can lead to identification of the circulating strains and often a sexual network in a community to help in designing the control strategy. Keeping in mind the above consideration, a total of 239 N. gonorrhoeae, isolated from high-risk populations, were characterized for serotypes and serovars by monoclonal antibodies against protein 1 of the organism. Majority of the serotypes were serotype B (142, 59.4%). Majority of the isolates showing resistance to at least one of the antibiotics tested were also serotype B (139, 59.2%), whereas, majority of the isolates showing resistance to any three of the antibiotics (multidrug resistant, MDR) (63%) was serotype A. A total of 41 different serovars were also identified and five of which (Arst, Bropt, Bopt, Arost, and Brop) included the highest percent (49.3%) of the isolates. Many serovars (23/41, 56.1%) were new emergent and included 58 (24.3%) of the isolates investigated. All of the new serovars were resistant to at least one of the antibiotics tested and the highest rate (40/102, 39.2%) was MDR. Serotyping and serovar determination was found contributory to understand the microepidemics of the N. gonorrhoeae isolates. Further studies including antibiogram and contact tracing can efficiently help in control of the disease. PMID:23540188

  2. Methods of making organic compounds by metathesis

    Energy Technology Data Exchange (ETDEWEB)

    Abraham, Timothy W.; Kaido, Hiroki; Lee, Choon Woo; Pederson, Richard L.; Schrodi, Yann; Tupy, Michael John

    2015-09-01

    Described are methods of making organic compounds by metathesis chemistry. The methods of the invention are particularly useful for making industrially-important organic compounds beginning with starting compositions derived from renewable feedstocks, such as natural oils. The methods make use of a cross-metathesis step with an olefin compound to produce functionalized alkene intermediates having a pre-determined double bond position. Once isolated, the functionalized alkene intermediate can be self-metathesized or cross-metathesized (e.g., with a second functionalized alkene) to produce the desired organic compound or a precursor thereto. The method may be used to make bifunctional organic compounds, such as diacids, diesters, dicarboxylate salts, acid/esters, acid/amines, acid/alcohols, acid/aldehydes, acid/ketones, acid/halides, acid/nitriles, ester/amines, ester/alcohols, ester/aldehydes, ester/ketones, ester/halides, ester/nitriles, and the like.

  3. The heart as an endocrine organ

    OpenAIRE

    Ogawa, Tsuneo; Adolfo J. De Bold

    2014-01-01

    The concept of the heart as an endocrine organ arises from the observation that the atrial cardiomyocytes in the mammalian heart display a phenotype that is partly that of endocrine cells. Investigations carried out between 1971 and 1983 characterised, by virtue of its natriuretic properties, a polypeptide referred to atrial natriuretic factor (ANF). Another polypeptide isolated from brain in 1988, brain natriuretic peptide (BNP), was subsequently characterised as a second hormone produced by...

  4. Quorum sensing antagonism from marine organisms

    DEFF Research Database (Denmark)

    Skindersø, Mette Elena; Ettinger-Epstein, P.; Rasmussen, Thomas Bovbjerg;

    2008-01-01

    target for the control of infectious bacteria. We present the results of screening of 284 extracts of marine organisms from the Great Barrier Reef, Australia, for their inhibition of QS. Of the 284 extracts, 64 (23%) were active in a general, LuxR-derived QS screen, and of these 36 (56%) were also active...... in a specific Pseudomonas aeruginosa QS screen. Extracts of the marine sponge Luffariella variabilis proved active in both systems. The secondary metabolites manoalide, manoalide monoacetate, and secomanoalide isolated from the sponge showed strong QS inhibition of a lasB::gfp(ASV) fusion......, demonstrating the potential for further identification of specific QS antagonists from marine organisms....

  5. In Vitro Susceptibility Test of Different Clinical Isolates against Ceftriaxone

    Directory of Open Access Journals (Sweden)

    Syed Hakim Masood

    2010-06-01

    Full Text Available Objectives: Because of the prevailing penicillin resistance in microorganisms, broad spectrum cephalosporins are used empirically specially in developing countries. The aim of this study is to determine the susceptibility pattern of different gram positive and gram negative pathogens against third generation cephalosporin-ceftriaxone to explore the existing effectiveness of this antibiotic.Methods: 180 clinical isolates of different gram positive and gram negative pathogens including P.mirabilis, S. typhi P.aeruginosa, E. coli, S. aureus and Klebsiella were collected from blood and urine samples of in-patients. 30 isolates of all species were tested against each of six brands of ceftriaxone using in vitro sensitivity tests by disc diffusion method (NCCLS criteria. The susceptibility limit was ≥21 mm zone of inhibition, while moderately susceptible was considered at 20-14 mm, and those isolates which showed >13 mm or no zone of inhibition were resistant to this antibacterial drug.Results: Ceftriaxone was found most effective against S. aureus. While 96.1% of the isolates showed susceptibility towards ceftriaxone, followed by E. coli (95%, P. aeruginosa (92.7%, K. pneumonia (89.4% and S. typhi (87.2%. P. mirabilis showed lowest susceptibility amongst all the test organisms (83.8%.Conclusion: Ceftriaxone can be used as a drug of choice in infections caused by S. aureus, E. coli, P. aurigenosa, K. pneumonia and S. typhi. However, it should be used with other antimicrobial agents in order to increase its effectiveness against P. mirabilis.

  6. Isolation and characterization of three mammalian orthoreoviruses from European bats.

    Directory of Open Access Journals (Sweden)

    Claudia Kohl

    Full Text Available In recent years novel human respiratory disease agents have been described in South East Asia and Australia. The causative pathogens were classified as pteropine orthoreoviruses with strong phylogenetic relationship to orthoreoviruses of flying foxes inhabiting these regions. Subsequently, a zoonotic bat-to-human transmission has been assumed. We report the isolation of three novel mammalian orthoreoviruses (MRVs from European bats, comprising bat-borne orthoreovirus outside of South East Asia and Australia and moreover detected in insectivorous bats (Microchiroptera. MRVs are well known to infect a broad range of mammals including man. Although they are associated with rather mild and clinically unapparent infections in their hosts, there is growing evidence of their ability to also induce more severe illness in dogs and man. In this study, eight out of 120 vespertilionid bats proved to be infected with one out of three novel MRV isolates, with a distinct organ tropism for the intestine. One isolate was analyzed by 454 genome sequencing. The obtained strain T3/Bat/Germany/342/08 had closest phylogenetic relationship to MRV strain T3D/04, isolated from a dog. These novel reoviruses provide a rare chance of gaining insight into possible transmission events and of tracing the evolution of bat viruses.

  7. Screening and isolation of halophilic bacteria producing industrially important enzymes

    Directory of Open Access Journals (Sweden)

    Sumit Kumar

    2012-12-01

    Full Text Available Halophiles are excellent sources of enzymes that are not only salt stable but also can withstand and carry out reactions efficiently under extreme conditions. The aim of the study was to isolate and study the diversity among halophilic bacteria producing enzymes of industrial value. Screening of halophiles from various saline habitats of India led to isolation of 108 halophilic bacteria producing industrially important hydrolases (amylases, lipases and proteases. Characterization of 21 potential isolates by morphological, biochemical and 16S rRNA gene analysis found them related to Marinobacter, Virgibacillus, Halobacillus, Geomicrobium, Chromohalobacter, Oceanobacillus, Bacillus, Halomonas and Staphylococcus genera. They belonged to moderately halophilic group of bacteria exhibiting salt requirement in the range of 3-20%. There is significant diversity among halophiles from saline habitats of India. Preliminary characterization of crude hydrolases established them to be active and stable under more than one extreme condition of high salt, pH, temperature and presence of organic solvents. It is concluded that these halophilic isolates are not only diverse in phylogeny but also in their enzyme characteristics. Their enzymes may be potentially useful for catalysis under harsh operational conditions encountered in industrial processes. The solvent stability among halophilic enzymes seems a generic novel feature making them potentially useful in non-aqueous enzymology.

  8. Organic waste treatment with organically modified clays

    International Nuclear Information System (INIS)

    The use of organically modified clays in hazardous waste management applications offers a significant new and untapped potential. These clays may be used in the stabilization of organic wastes and organically contaminated soils, for waste water treatment, for oil spill control, for liner systems beneath fuel oil storage tanks, and as a component within liner systems of hazardous waste storage treatment and disposal facilities. Organically modified clays (organophilic clays) may be employed in each of these systems to adsorb organic waste constituents, enhancing the performance of the applications

  9. Genetic diversity of Trichomonas vaginalis clinical isolates determined by EcoRI restriction fragment length polymorphism of heat-shock protein 70 genes.

    Science.gov (United States)

    Meade, John C; de Mestral, Jacqueline; Stiles, Jonathan K; Secor, W Evan; Finley, Richard W; Cleary, John D; Lushbaugh, William B

    2009-02-01

    Restriction fragment length polymorphism (RFLP) analysis using a multilocus heat-inducible cytoplasmic heat-shock protein 70 (Hsp70) hybridization probe with EcoRI-digested genomic DNA was used in molecular typing of 129 Trichomonas vaginalis isolates. Results indicate that Trichomonas organisms exhibit considerable polymorphism in their Hsp70 RFLP patterns. Analysis of seven American Type Culture Collection reference strains and 122 clinical isolates, including 84 isolates from Jackson, Mississippi, 18 isolates from Atlanta, Georgia, and 20 isolates from throughout the United States, showed 105 distinct Hsp70 RFLP pattern subtypes for Trichomonas. Phylogenetic analysis of the Hsp70 RFLP data showed that the T. vaginalis isolates were organized into two clonal lineages. These results illustrate the substantial genomic diversity present in T. vaginalis and indicate that a large number of genetically distinct Trichomonas isolates may be responsible for human trichomoniasis in the United States. PMID:19190222

  10. Slime production a virulence marker in Pseudomonas aeruginosa strains isolated from clinical and environmental specimens: A comparative study of two methods

    Directory of Open Access Journals (Sweden)

    Prasad S

    2009-04-01

    Full Text Available Detection of slime in Pseudomonas aeruginosa can be useful in understanding the virulence of this organism. Here, comparative studies of two phenotypic methods using the tube method and the spectrophotometric method for slime production from 100 clinically and 21 environmentally significant isolates of P. aeruginosa were performed. A total of 68 isolates were positive by either of the tests whereas only 34 were positive by both the tests. The tube method detected slime significantly in more number of isolates than the spectrophotometric method. The tube test was found to be superior to the spectrophotometric method in ease of performance, interpretation and sensitivity. Among the clinical isolates, systemic isolates produce less slime compared to wound, respiratory and urinary isolates. Isolates from the hospital environment produced more slime indicating that this virulence marker helps the organism to survive for longer periods and cause nosocomial infections.

  11. Molecular characterization of Danish Cryptosporidium parvum isolates

    DEFF Research Database (Denmark)

    Enemark, Heidi L.; Ahrens, Peter; Juel, Cynthia Dawn;

    2002-01-01

    characterized as C. meleagridis. The porcine Cryptosporidium isolates (N = 4) revealed a pattern which was genetically distinct from human and bovine isolates. Cryptosporidium in a hedgehog (Erinaceus europaeus L.) was identified for the first time. By microsatellite sequencing the hedgehog isolate showed a...

  12. Vibrio cholerae O1 isolated in Kenya.

    OpenAIRE

    Iwanaga, M; Mori, K.; Kaviti, J N

    1982-01-01

    Biological and serological analyses of 272 isolates of Vibrio cholerae O1 from six epidemics and from a few sporadic cases in Kenya were carried out. All of the isolates were identified as V. cholerae biotype E1 Tor, and 210 out of 272 isolates were hemolytic as examined by Feeley's method.

  13. Volatile Organic Compounds (VOCs)

    Science.gov (United States)

    ... Organic Compounds' Impact on Indoor Air Quality Volatile Organic Compounds' Impact on Indoor Air Quality On this page: ... Exposure Standards or Guidelines Additional Resources Introduction Volatile organic compounds (VOCs) are emitted as gases from certain solids ...

  14. Isolation of NDM-1-producing Klebsiella pnemoniae in Ireland, July 2011.

    LENUS (Irish Health Repository)

    McDermott, H

    2012-01-01

    We report the identification of New Delhi metallo-betalactamase 1 (NDM-1)-producing Klebsiella pnemoniae in Ireland. The organism was resistant to multiple antibiotic classes, including carbapenems, and PCR and sequencing confirmed the presence of the blaNDM-1 gene, carried on a 98 kb plasmid. The organism was isolated from an infant, who was born in India and moved to Ireland at the age of four months. This is the first reported isolation of an NDM-1-producing Enterobacteriaceae strain in Ireland.

  15. Ashes for organic farming

    OpenAIRE

    Kousa, T.; Heinonen, M; Suoniitty, T.; Peltonen, K

    2013-01-01

    Nowadays only eight percent of the cultivated field area is used for organic farming. The Ministry of Agriculture and Forestry has published the guidelines for the program of organic farming to diversify the supply and the consumption of organic food. The aim is to increase organically arable land to 20% by the year 2020.The demand of organic fertilizer products is strongly increasing. Interest in forestry by-products (ash, bark, zero fiber, etc.) for use in organic production has recently be...

  16. Organic Viticulture in Europe

    OpenAIRE

    Willer, Helga; Häseli, Andreas; Levite, Dominique; Tamm, Lucius

    2002-01-01

    Introduction Organic agriculture is a thriving sector in Europe, and in some countries 10 % of the agricultural area is organic. Not so, however, for organic viticulture: the percentage of organic viticulture land compared to all vineyards is lower than for organic agriculture. This is due to several constraints in the sector of production techniques, in marketing, legislation and state support. Material and Methods So far no official data on the current status of organic viticultur...

  17. Can particulate organic matter reveal emerging changes in soil organic carbon?

    DEFF Research Database (Denmark)

    Simonsson, Magnus; Kirchmann, Holger; Magid, Jakob;

    2014-01-01

    This study assessed whether particulate organic matter (POM) in sand fractions, isolated by wet sieving after treatment with Na hexametaphosphate, can be a sensitive indicator of incipient changes in the content and composition of soil organic matter. In five long-term field experiments including....... Although organic matter in Fraction B had a higher intrinsic sensitivity to soil management, which was partly able to overcome the larger errors, we concluded that an observer would be more likely to detect changes by measuring total organic C and N, when monitoring decadal changes in C and N pools....... This makes the investigated POM fractions less suitable as indicators for changes in soil C stocks. However, the C/N ratio of Fraction B showed a distinct signature of the history of organic matter input to the soil, which was absent in the C/N ratio of the total fine earth. © Soil Science Society of America...

  18. Spoilage potential of Pseudomonas species isolated from goat milk.

    Science.gov (United States)

    Scatamburlo, T M; Yamazi, A K; Cavicchioli, V Q; Pieri, F A; Nero, L A

    2015-02-01

    Pseudomonas spp. are usually associated with spoilage microflora of dairy products due to their proteolytic potential. This is of particular concern for protein-based products, such as goat milk cheeses and fermented milks. Therefore, the goal of the present study was to characterize the proteolytic activity of Pseudomonas spp. isolated from goat milk. Goat milk samples (n=61) were obtained directly from bulk tanks on dairy goat farms (n=12), and subjected to a modified International Organization for Standardization (ISO) protocol to determine the number and proteolytic activity of Pseudomonas spp. Isolates (n=82) were obtained, identified by PCR, and subjected to pulsed-field gel electrophoresis with XbaI macro-restriction. Then, the isolates were subjected to PCR to detect the alkaline protease gene (apr), and phenotypic tests were performed to check proteolytic activity at 7°C, 25°C, and 35°C. Mean Pseudomonas spp. counts ranged from 2.9 to 4.8 log cfu/mL, and proteolytic Pseudomonas spp. counts ranged from 1.9 to 4.6 log cfu/mL. All isolates were confirmed to be Pseudomonas spp., and 41 were identified as Pseudomonas fluorescens, which clustered into 5 groups sharing approximately 82% similarity. Thirty-six isolates (46.9%) were positive for the apr gene; and 57 (69.5%) isolates presented proteolytic activity at 7°C, 82 (100%) at 25°C, and 64 (78%) at 35°C. The isolates were distributed ubiquitously in the goat farms, and no relationship among isolates was observed when the goat farms, presence of apr, pulsotypes, and proteolytic activity were taken into account. We demonstrated proteolytic activity of Pseudomonas spp. present in goat milk by phenotypic and genotypic tests and indicated their spoilage potential at distinct temperatures. Based on these findings and the ubiquity of Pseudomonas spp. in goat farm environments, proper monitoring and control of Pseudomonas spp. during production are critical. PMID:25497792

  19. Occurrence of heavy metals and antibiotic resistance in bacteria from internal organs of american bullfrog (Rana catesbeiana) raised in Malaysia

    OpenAIRE

    Lee SW; M. Najiah; W Wendy; M Nadirah; SH Faizah

    2009-01-01

    A total of 40 bacteria have been successfully isolated from internal organs of the American bullfrog (Rana catesbeiana) raised in Malaysia, namely, eight isolates of Aeromonas spp., 21 of Edwardsiella spp., six of Flavobacterium spp. and five of Vibrio spp. In terms of antibiotic susceptibility testing, each isolate was tested against 21 antibiotics, resulting in 482 (57.3%) cases of sensitivity and 61 (7.3%) cases of partial sensitivity. Meanwhile, 297 (35.4%) bacterial isolates were registe...

  20. Organic brain syndrome

    Science.gov (United States)

    OBS; Organic mental disorder (OMS); Chronic organic brain syndrome ... Listed below are disorders associated with OBS. Brain injury caused by ... the brain ( subarachnoid hemorrhage ) Blood clot inside the ...

  1. Isolated pancreatic injury following blunt abdominal trauma in a child

    Directory of Open Access Journals (Sweden)

    Jain Sandeep

    2007-01-01

    Full Text Available Pancreatic injury following blunt abdominal trauma is rare as compared to other visceral organs. Isolated injury to the pancreas is even more rare. The clinical presentation is subtle resulting in delayed treatment with high morbidity and mortality. A three-year-old female child presented with vomiting 18h following a motor vehicle accident. She was hemodynamically stable with no external signs of injury. Investigations revealed hyperamylasemia and isolated grade III pancreatic injury. Laparotomy with distal pancreatic resection and splenectomy was done. A high degree of clinical suspicion with due consideration to the mechanism of injury is the key to good outcome in these patients. Major ductal injury is the critical issue in the management and a number of therapeutic choices are available specific to the location of the insult.

  2. BIODEGRADATION OF PETROLEUM BY FUNGI ISOLATED FROM UNPOLLUTED TROPICAL SOIL

    Directory of Open Access Journals (Sweden)

    Damisa, D

    2013-03-01

    Full Text Available Bioremediation studies on the capabilities of Aspergillus niger A1, Candida sp C10 and Rhizopus stolonifer R7 isolated from unpolluted soil in Minna, Niger State Nigeria was investigated and rate of degradation measured by weight loss. Aspergillus niger A1, exhibited the highest ability in degrading the crude oil than Candida sp C10, and Rhizopus stolonifer R7. Aspergillus niger A1 degraded 53.7% of the crude oil after 16 days period of incubation while Candida sp C10 and Rhizopus stolonifer R7 degraded 45.0% and 35.0% respectively over the same period of incubation.. The result obtained demonstrated that the three fungi isolates are competent petroleum degrading organisms and may be used as best approaches to restoring oil contaminated environments through bioremediation process.

  3. [Isolation of proteins in coconut water].

    Science.gov (United States)

    Birosel, D M; de Oliveira Ferro, V; Holcberg, I B; Pitelli, A C

    1976-01-01

    The isolation of protein fractions in cocont water was achieved by precipitation with controlled pH variation obtaining three isolates at pH 8,5, 10,5, and 11,5. By comparing each of these isolates with proteins of coconut milk, a similarity between properties of the first two isolates of the water - pH 8,5, 10,5--and those of coconut serum proteins -- glutelin and prolamin was observed. The third isolate is entirely absent from the milk, when coconut water is not used in the second pressing to obtain the milk. PMID:19819

  4. Simplified design procedure for base isolation system

    Energy Technology Data Exchange (ETDEWEB)

    Takayama, Mineo; Tada, Hideyuki [Fukuoka Univ. (Japan). Faculty of Engineering

    1995-12-01

    This paper presents a simplified design procedure which incorporates an existing response prediction method for base isolated buildings into design methods for isolation devices (rubber bearings and hysteresis dampers). The procedure enables a designer to easily identify the relationship between tile seismic behavior of base-isolated buildings and the characteristics of isolation devices. The prediction method, proposed by Prof. Akiyama, is based on energy balance between the total input seismic energy and the energy absorbed by the isolation devices. The method is very accurate. The design methods for devices were developed by authors based on experimental and finite element analysis results.

  5. Fungi isolated in school buildings

    Directory of Open Access Journals (Sweden)

    Elżbieta Ejdys

    2013-12-01

    Full Text Available The aim of the study was to determine the species composition of fungi occurring on wall surfaces and in the air in school buildings. Fungi isolated from the air using the sedimentation method and from the walls using the surface swab technique constituted the study material. Types of finish materials on wall surfaces were identified and used in the analysis. Samples were collected in selected areas in two schools: classrooms, corridors, men's toilets and women's toilets, cloakrooms, sports changing rooms and shower. Examinations were conducted in May 2005 after the heating season was over. Fungi were incubated on Czapek-Dox medium at three parallel temperatures: 25, 37 and 40°C, for at least three weeks. A total of 379 isolates of fungi belonging to 32 genera of moulds, yeasts and yeast-like fungi were obtained from 321 samples in the school environment. The following genera were isolated most frequently: Aspergillus, Penicillium and Cladosporium. Of the 72 determined species, Cladosporium herbarum, Aspergillus fumigatus and Penicillium chrysogenum occurred most frequently in the school buildings. Wall surfaces were characterised by an increased prevalence of mycobiota in comparison with the air in the buildings, with a slightly greater species diversity. A certain species specificity for rough and smooth wall surfaces was demonstrated. Fungi of the genera Cladosporium and Emericella with large spores adhered better to smooth surfaces while those of the genus Aspergillus with smaller conidia adhered better to rough surfaces. The application of three incubation temperatures helped provide a fuller picture of the mycobiota in the school environment.

  6. Basalt waste isolation project institutional activities

    International Nuclear Information System (INIS)

    The Basalt Waste Isolation Project (BWIP) was established in 1976 as part of the National Waste Terminal Storage Program, and characterization studies to evaluate the Hanford Site have been ongoing since that time. In early 1983, the Nuclear Waste Policy Act (NWPA) became law and established the Office of Civilian Radioactive Waste Management, which includes the BWIP as part of that program. This paper describes the interaction process required by the NWPA and has been ongoing for the past 3 to 4 yr with the State of Washington, Yakima Indian Nation, Confederated Tribes of the Umatilla Indian Reservation, Nez Perce Tribe, other federal agencies, city and county governments, the public and news media, and many other organizations. It identifies the issues that the state and the US Department of Energy (DOE) have noted during the consultation and cooperation (C and C) process. In addition, this paper covers the site recommendation/nomination process for the first repository, including number of sites, location, and types of media being investigated. It covers the site recommendation/nomination process for the second repository, including types of media and where those investigations are being performed. Discussion covering the OCRWM program strategy, which includes documentation being prepared for site recommendation/nomination, site investigations required to determine site suitability, the licensing process for the first repository, etc., are provided

  7. Speciation of coagulase negative Staphylococcal isolates from clinically significant specimens and their antibiogram

    Directory of Open Access Journals (Sweden)

    M G Usha

    2013-01-01

    Full Text Available Background: Despite their frequency as contaminants, coagulase-negative Staphylococci (CONS have become important nosocomial pathogens, accounting for 9% of all nosocomial infections. These infections are difficult to treat because of the risk factors and the multiple drug resistance nature of these organisms. Materials and Methods: One hundred and two consecutive CONS were isolated from various clinical samples like blood, pus, urine, urine catheter tip and gastric lavage. Most of the blood samples were from patients with risk factors (immunocompromised or on medical devices. After confirming the isolates as CONS, species-level identification was performed by simple, non-expensive conventional methods and antibiotic sensitivity testing was also carried out. Results: Of 102 CONS isolates, 100 isolates could be identified to the species level. Among the 100 isolates, epidermidis was the most common species isolated, seen in 32%, followed by S. hemolyticus (18%, S. lugdunensis (12%, S. hominis (10%, S. saprophyticus (8%, S. capitis (6%, S. caprae (4%, S. xylosus (4%, S. cohni and S. warneri (3% each. In the present study, 56% of the isolates were methicillin-resistant CONS. Most of the isolates showed resistance to ampicillin and amoxyclav (89% each, followed by ceftriaxone (52%, cotrimoxazole (46%, cefotaxime (32%, gentamicin (25%, amikacin (21%. Conclusion: The increased pathogenic potential and multiple-drug resistance demonstrates the need to adopt simple, reliable and non-expensive methods for identifying and determining the antibiotic sensitivity of CONS.

  8. Cloning and Clone Analysis of GRA1 Gene from Local Isolate Toxoplasma gondii Tachyzoite

    Directory of Open Access Journals (Sweden)

    Didik T Subekti

    2008-03-01

    Full Text Available The GRA1 gene of Toxoplasma gondii encoding protein called GRA1 protein. GRA1 protein known to be immunogenic and essentialy involved in modification of parasitophorus vacoule which has role in immune evasion and virulency of organism. The local isolate of T. gondii is successfuly isolated and known as highly pathogenic isolate similarly as its RH strain. Unfortunately, the homology sequence of GRA1 gene between those isolate still unknown. The purpose of the research are to clone the GRA1 gene and to analyze the homology from pathogenic T. gondii isolate and RH strain. Tachyzoite of T. gondii was grown in mice peritoneum by intraperitoneal injection. Then, total mRNA was isolated and purified. cDNA was synthesized from mRNA and then amplified using F1 dan R1 primers to get clone of GRA1 from local isolate. Homology analysis was perform using several bioinformatic softwares. The result showed that cDNA of GRA1 from local isolate has 84% homologs with RH strain of T.gondii. However, when subsequently editing performed to parts of suspected non coding sequence of cDNA GRA1 to get CDS of GRA1, the homology was increase to 100% compare to CDS of GRA1 of RH strain.

  9. Detection of Intracellular Adhesion (ica Gene and Biofilm Formation Staphylococcus aureus Isolates from Clinical Blood Cultures

    Directory of Open Access Journals (Sweden)

    Mohsen Mirzaee

    2015-10-01

    Full Text Available Background: In fact the biofilms are composed of bacterial cells living inmulticellular structures such as tissues and organs embedded within a self-produced matrix of extracellular polymeric substance (EPS. Ability to attach and biofilm formation are the most important virulence factors Staphylococcus aureus isolates. The aims of this study were to detect intracellular adhesion (ica locus and its relation to the biofilm formation phenotype in clinical isolates of S. aureus isolated from bloodcultures.Methods: A total of 31 clinical S. aureus isolates were collected from Loghman Hospital of Tehran, Iran. In vitro biofilm formation ability was determined by microliter tissue culture plates. All clinical isolates were examined for determination the ica locus by using PCR method.Results: Twelve (38.7% of the isolates were strong biofilm producers. The results showed that 18(80.6% of the isolates carried icaD gene, whereas the prevalence of icaA, icaB and icaC were 51.6%, 45.1% and 77.4% respectively.Conclusions: S. aureus clinical isolates have different ability to form biofilm. This may be caused by the differences in the expression of biofilm related genes, genetic make-up and physiological conditions.

  10. Biochemical and Molecular Characterization of Carotenogenic Flavobacterial Isolates from Marine Waters.

    Science.gov (United States)

    Sowmya, Rama; Sachindra, Nakkarike M

    2016-01-01

    Carotenoids are known to possess immense nutraceutical properties and microorganisms are continuously being explored as natural source for production of carotenoids. In this study, pigmented bacteria belonging to Flavobacteriaceae family were isolated using kanamycin-containing marine agar and identified using the molecular techniques and their phenotypic characteristics were studied along with their potential to produce carotenoids. Analysis of random amplification of polymorphic DNA (RAPD) banding patterns and the fragment size of the bands indicated that the 10 isolates fall under two major groups. Based on 16S rRNA gene sequence analysis the isolates were identified as Vitellibacter sp. (3 isolates), Formosa sp. (2 isolates) and Arenibacter sp. (5 isolates). Phenotypically, the isolates showed slight variation from the reported species of these three genera of Flavobacteriaceae. Only the isolates belonging to Vitellibacter and Formosa produced flexirubin, a typical yellow orange pigment produced by most of the organisms of the family Flavobacteriaceae. Vitellibacter sp. and Formosa sp. were found to produce higher amount of carotenoids compared to Arenibacter sp. and zeaxanthin was found to be the major carotenoid produced by these two species. The study indicated that Vitellibacter sp. and Formosa sp. can be exploited for production of carotenoids, particularly zeaxanthin. PMID:27281997

  11. Isolation and characterization of entomopathogenic bacteria from soil samples from the western region of Cuba.

    Science.gov (United States)

    González, Aileen; Rodríguez, Graciela; Bruzón, Rosa Y; Díaz, Manuel; Companionis, Ariamys; Menéndez, Zulema; Gato, René

    2013-06-01

    The use of insect pathogens is a viable alternative for insect control because of their relative specificity and lower environmental impact. The search for wild strains against dipterans could have an impact on mosquito control programs. We have made an extensive screening of soil in western Cuba to find bacteria with larvicidal activity against mosquitoes. A total of 150 soil samples were collected and isolates were identifying using the API 50 CHB gallery. Phenotypic characteristics were analyzed by hierarchical ascending classification. Quantitative bioassays were conducted under laboratory conditions following the World Health Organization protocol in order to ascertain the toxicity and efficacy of isolates. The protein profiles of the crystal components were determined by SDS-PAGE. Eight hundred and eighty-one bacterial isolates were obtained, and 13 isolates with entomopathogenic activity were isolated from nine samples. Nine isolates displayed higher entomopathogenic activity against both Cx. quinquefasciatus and Ae. aegypti compared with the reference strain 266/2. All toxic isolates showed higher biological potency than the 266/2 strain. These isolates with high entomopathogenic activity displayed a protein pattern similar to the B. thuringiensis var. israelensis IPS-82 and 266/2 strains. These results are a valuable tool for the control of Diptera of medical importance. PMID:23701606

  12. Evaluation of extended spectrum beta lactamase enzymes prevalence in clinical isolates of Escherichia coli

    Directory of Open Access Journals (Sweden)

    Ronak Bakhtiari

    2011-10-01

    Full Text Available Resistance to b-lactam antibiotics by gramnegative bacteria, especially Escherichia coli (E. coli, is a major public health issue worldwide. The predominant resistance mechanism in gram negative bacteria particularly E. coli is via the production of extended spectrum beta lactamase (ESBLs enzymes. In recent years, the prevalence of b-lactamase producing organisms is increased and identification of these isolates by using disk diffusion method and no-one else is not satisfactory. So, this investigation focused on evaluating the prevalence of ESBL enzymes by disk diffusion method and confirmatory test (Combined Disk. Five hundred clinical samples were collected and 200 E. coli isolates were detected by standard biochemical tests. To performing initial screening of ESBLs was used from Disk diffusion method on E. coli isolates. A confirmation test (Combined Disk method was performed on isolates of resistant to cephalosporin's indicators. Up to 70% isolates exhibited the Multi Drug Resistance phenotype. In Disk diffusion method, 128(64% E. coli isolates which resistant to ceftazidime and cefotaxime while in Combined Disk, among 128 screened isolates, 115 (89.8% isolates were detected as ESBLs producers. This survey indicate beta lactamase enzymes are playing a significant role in antibiotic resistance and correct detection of them in phenotypic test by using disk diffusion and combined Disk is essential for accurate recognition of ESBLs.

  13. Genetic relatedness of Legionella longbeachae isolates from human and environmental sources in Australia

    International Nuclear Information System (INIS)

    The genetic relatedness of Legionella longbeachae isolated in Australia since 1987 was investigated by restriction fragment length polymorphism (RFLP) analysis and allozyme electrophoresis. Three radiolabeled probes were used in Southern hybridizations for the RFLP studies. They were Escherichia coli 16S and 23S rRNA and cloned fragments of L. longbeachae selected empirically from genomal banks in lambda and a cosmid. The legionellae included in the study comprised 11 Legionella longbeachae serogroup 1 organisms isolated form humans, 28 L. longbeachae serogroup 1 isolates from environmental sources, 3 L. longbeachae serogroup 2 environmental isolates. These were compared with the American Type Culture Collection reference strains of both serogroups and some other related Legionella species. Results of allozyme and RFLP analysis showed that all the isolates from humans and all but three of the environmental L. longbeachae serogroup 1 isolates were closely related. They were also closely related to L. longbeachae serogroup 1 ATCC 33462. There was wider variation among the three L. longbeachae serogroup 2 environmental isolates. One of these was closely related to L. longbeachae serogroup 2 ATCC 33484. RFLP studies with the rRNA probe provided the most discrimination among isolates but did not distinguish between the two serogroups

  14. Thermodynamic laws in isolated systems.

    Science.gov (United States)

    Hilbert, Stefan; Hänggi, Peter; Dunkel, Jörn

    2014-12-01

    The recent experimental realization of exotic matter states in isolated quantum systems and the ensuing controversy about the existence of negative absolute temperatures demand a careful analysis of the conceptual foundations underlying microcanonical thermostatistics. Here we provide a detailed comparison of the most commonly considered microcanonical entropy definitions, focusing specifically on whether they satisfy or violate the zeroth, first, and second laws of thermodynamics. Our analysis shows that, for a broad class of systems that includes all standard classical Hamiltonian systems, only the Gibbs volume entropy fulfills all three laws simultaneously. To avoid ambiguities, the discussion is restricted to exact results and analytically tractable examples. PMID:25615053

  15. [Neuroanatomy of Isolated Body Lateropulsion].

    Science.gov (United States)

    Nakazato, Yoshihiko; Tamura, Naotoshi; Ikeda, Kei; Tanaka, Ai; Yamamoto, Toshimasa

    2016-03-01

    Axial body lateropulsion, a phenomenon where the body is pulled toward the side of the lesion, with tendency of falling down, is the well-known transient feature of lateral medullary syndrome. In some cases, axial body lateropulsion occurs without vestibular and cerebellar symptoms (isolated body lateropulsion:[iBL]). Patients with iBL have a lesion located in the spinocerebellar tract, descending lateral vestibulospinal tract, vestibulo-thalamic pathway, dentatorubrothalamic pathway, or thalamocortical fascicle. This review deals with the anatomic basis and clinical significance of iBL. PMID:27001775

  16. Basalt waste isolation project overview

    International Nuclear Information System (INIS)

    The proposed candidate site for a high-level nuclear waste repository is located beneath the Hanford Nuclear Reservation in southeastern Washington State. At this point, the Hanford Reservation has been selected as one of three preferred candidates in the draft environmental assessment. Project activities have concentrated on understanding the site location with respect to the 10CFR60, 40CFR191, and 10CFR960, identifying critical parameters for design of water package and repository seals, and identifying parameters for repository design. This paper describes the program to evaluate the site and to identify the natural processes that would effect isolation

  17. Isolation, speciation, and antibiogram of clinically relevant non-diphtherial Corynebacteria (Diphtheroids

    Directory of Open Access Journals (Sweden)

    B S Reddy

    2012-01-01

    Full Text Available Purpose: Coryneform or the non-diphtherial Corynebacterium species largely remains a neglected group with the traditional consideration of these organisms as contaminants. This concept, however, is slowly changing in the light of recent observations. This study has been done to find out the species distribution and antibiogram of various members of the clinically relevant Coryneform group, isolated from various clinical materials. Materials and Methods: One hundred and fourteen non-duplicate isolates of diphtheroids from various clinical isolates were selected for the study. The isolates were identified to the species level by using a battery of tests; and antimicrobial susceptibility was tested by using a combination of Clinical and Laboratory Standards Institute (CLSI and the British Society for Antimicrobial Chemotherapy (BSAC guidelines, in the absence of definitive CLSI guidelines. Results: Corynebacterium amycolatum was the predominant species (35.9% in our series followed by the CDC Group G organisms (15.7%. Each of the remaining 19 species comprised of less than 10% of the isolates. More than half the total isolates were resistant to the penicillins, erythromycin, and clindamycin; while excellent activity (all the strains being susceptible was shown by vancomycin, linezolid, and tigecycline. Chloramphenicol and tetracycline also had good activity in inhibiting more than 80% of the isolates. Multiply drug resistance was exhibited by all the species. Conclusion: This study was an attempt to establish the clinical significance of coryneform organisms. The high level of resistance shown by this group to some of the common antibacterial agents highlights the importance of processing these isolates in select conditions to guide the clinicians towards an appropriate therapy.

  18. Cultural conditions on the production of extracellular enzymes by Trichoderma isolates from tobacco rhizosphere

    Directory of Open Access Journals (Sweden)

    K.L.N Mallikharjuna Rao

    2016-03-01

    Full Text Available Abstract Twelve isolates of Trichoderma spp. isolated from tobacco rhizosphere were evaluated for their ability to produce chitinase and β-1,3-glucanase extracellular hydrolytic enzymes. Isolates ThJt1 and TvHt2, out of 12 isolates, produced maximum activities of chitinase and β-1,3-glucanase, respectively. In vitro production of chitinase and β-1,3-glucanase by isolates ThJt1 and TvHt2 was tested under different cultural conditions. The enzyme activities were significantly influenced by acidic pH and the optimum temperature was 30 °C. The chitin and cell walls of Sclerotium rolfsii, as carbon sources, supported the maximum and significantly higher chitinase activity by both isolates. The chitinase activity of isolate ThJt1 was suppressed significantly by fructose (80.28%, followed by glucose (77.42%, whereas the β-1,3-glucanase activity of ThJt1 and both enzymes of isolate TvHt2 were significantly suppressed by fructose, followed by sucrose. Ammonium nitrate as nitrogen source supported the maximum activity of chitinase in both isolates, whereas urea was a poor nitrogen source. Production of both enzymes by the isolates was significantly influenced by the cultural conditions. Thus, the isolates ThJt1 and TvHt2 showed higher levels of chitinase and β-1,3-glucanase activities and were capable of hydrolyzing the mycelium of S. rolfsii infecting tobacco. These organisms can be used therefore for assessment of their synergism in biomass production and biocontrol efficacy and for their field biocontrol ability against S. rolfsii and Pythium aphanidermatum infecting tobacco.

  19. Cultural conditions on the production of extracellular enzymes by Trichoderma isolates from tobacco rhizosphere.

    Science.gov (United States)

    Mallikharjuna Rao, K L N; Siva Raju, K; Ravisankar, H

    2016-01-01

    Twelve isolates of Trichoderma spp. isolated from tobacco rhizosphere were evaluated for their ability to produce chitinase and β-1,3-glucanase extracellular hydrolytic enzymes. Isolates ThJt1 and TvHt2, out of 12 isolates, produced maximum activities of chitinase and β-1,3-glucanase, respectively. In vitro production of chitinase and β-1,3-glucanase by isolates ThJt1 and TvHt2 was tested under different cultural conditions. The enzyme activities were significantly influenced by acidic pH and the optimum temperature was 30°C. The chitin and cell walls of Sclerotium rolfsii, as carbon sources, supported the maximum and significantly higher chitinase activity by both isolates. The chitinase activity of isolate ThJt1 was suppressed significantly by fructose (80.28%), followed by glucose (77.42%), whereas the β-1,3-glucanase activity of ThJt1 and both enzymes of isolate TvHt2 were significantly suppressed by fructose, followed by sucrose. Ammonium nitrate as nitrogen source supported the maximum activity of chitinase in both isolates, whereas urea was a poor nitrogen source. Production of both enzymes by the isolates was significantly influenced by the cultural conditions. Thus, the isolates ThJt1 and TvHt2 showed higher levels of chitinase and β-1,3-glucanase activities and were capable of hydrolyzing the mycelium of S. rolfsii infecting tobacco. These organisms can be used therefore for assessment of their synergism in biomass production and biocontrol efficacy and for their field biocontrol ability against S. rolfsii and Pythium aphanidermatum infecting tobacco. PMID:26887223

  20. Isolation of aerobic bacteria from ticks infested sheep in Iraq

    Institute of Scientific and Technical Information of China (English)

    Waleed Ibrahem Jalil; Mohammad Mushgil Zenad

    2016-01-01

    Objective: To highlight the presence of aerobic bacteria in live ticks infested sheep, in Diyala Governorate, Iraq. Methods: One hundred and thirty adult alive ticks were picked up from sheep which were reared in different farms in Diyala Governorate, Iraq, during the period from November 2012 to May 2013. Ticks were classified in the Natural History Museum in Baghdad. They were dissected aseptically for extraction of the salivary gland and mid-gut. The removed tissue from each organ was inoculated in buffer peptone water (1%) and incubated for 2 h at 37℃, to maintain weak and/or injured bacterial cells, then transmitted to nutrient broth incubated at 37℃ for 18 h. Culturing was done on three solid bacteriological media (nutrient, blood and McConkey agars), and then incubated at 37℃ for 24 h. Bacterial identification was performed by using multiple biochemical tests and API-20 strips. Data were analyzed by using Statistical Analysis System version 9.1, 2010. Chi-square test was used for comparison at significant level of P ≤0.05. Results: Two species of ticks were identified [Rhipicephalus (Boophilus) annulatus and Hyalomma turanicum]. High bacterial isolation rate was observed (483 isolates). A significant high isolation rate was recorded from Rhipicephalus annulatus (63.14%). Six bacterial species were identified [Escherichia coli (28.36%), Pseudomonas aeruginosa (18.01%), Bacillus cereus (14.69%), Staphylococcus aureus (13.66%), Citrobacter freundii (13.04%), and Enterobacter species (12.21%)]. Also the high bacterial isolation rates were recorded in the temperate months (November, March and April); these coin-cided with high reproductive performance of ticks. Conclusions: The high isolation rate of aerobic pathogens from ticks might reflect the active contribution of this arthropod in environmental contamination and increase the probability of transmitting bacterial pathogens to their hosts.

  1. Antimicrobial Resistance Patterns of Isolated Vibrio cholerae Strains

    Directory of Open Access Journals (Sweden)

    Hajia

    2016-02-01

    Full Text Available Background Cholera is a potentially life-threatening acute diarrheal disease caused by the toxigenic bacteria, Vibrio cholerae. Antibiotics should be selected using local antibiotic susceptibility testing patterns. Objectives This study was performed to identify the patterns of antimicrobial resistance in isolates collected from laboratory-confirmed cases of cholera during three years, from 2011 to 2013. Materials and Methods All isolates at the Health Reference Laboratory were tested by the Minimum Inhibitory Concentration (MIC Test using Liofilchem against ciprofloxacin, nalidixic acid, cefixime, ampicillin, tetracycline, trimethoprim-sulfamethoxazole, and erythromycin. The following organisms were used as quality control strains for MIC E-testing; Escherichia coli (ATCC 25922, Staphylococcus aureus (ATCC 29213, and Pseudomonas aeruginosa (ATCC 27853. Results Results of susceptibility testing showed complete sensitivity to ciprofloxacin, cefixime and amplicillin for both isolated Inaba and Ogawa serotypes except all isolated Inaba serotypes from year 2011, which were resistant to cefixime. These resistant Inaba serotypes were not isolated in the next year. Inaba serotypes showed an increased resistance rate of up to 100% to nalidixic acid, tetracycline and trimethoprim-sulfamethaxazone, while Ogawa serotypes were 100% sensitive at the end of year 2013. The susceptibility pattern of erytromycine was similar in these two types. Sensitivity to erythromycin was decreased in both Inaba and Ogawa serotypes. Conclusions The analyzed results indicate that tetracycline should not be considered as a first line antibiotic therapy for patients infected with Ogawa serotypes. Also, national guidelines for confirmation of cholera should be improved by responsible authorities to cover new resistance during outbreaks.

  2. Blackbody Radiation from Isolated Neptunes

    CERN Document Server

    Ginzburg, Sivan; Loeb, Abraham

    2016-01-01

    Recent analyses of the orbits of some Kuiper Belt objects hypothesize the presence of an undiscovered Neptune-size planet at a very large separation from the Sun. The energy budget of Neptunes on such distant orbits is dominated by the internal heat released by their cooling rather than solar irradiation (making them effectively "isolated"). The blackbody radiation that these planets emit as they cool may provide the means for their detection. Here we use an analytical toy model to study the cooling and radiation of isolated Neptunes. This model can translate a detection (or a null detection) to a constraint on the size and composition of the hypothesized "Planet Nine". Specifically, the thick gas atmosphere of Neptune-like planets serves as an insulating blanket which slows down their cooling. Therefore, a measurement of the blackbody temperature, $T_{\\rm eff}\\sim 50\\textrm{K}$, at which a Neptune emits can be used to estimate the mass of its atmosphere, $M_{\\rm atm}$. Explicitly, we find the relation $T_{\\r...

  3. Braking Index of Isolated Pulsars

    CERN Document Server

    Hamil, Oliver Q; Urbanec, Martin; Urbancova, Gabriela

    2016-01-01

    Isolated pulsars are rotating neutron stars with accurately measured angular velocities $\\Omega$, and their time derivatives that show unambiguously that the pulsars are slowing down. The commonly accepted view is that it arises through emission of magnetic dipole radiation (MDR) from a rotating magnetized body. The calculated energy loss by a rotating pulsar with a constant moment of inertia is assumed proportional to a model dependent power of $\\Omega$. This relation leads to the power law $\\dot{\\Omega}$ = -K $\\Omega^{\\rm n}$ where $n$ is called the braking index. The MDR model predicts $n$ exactly equal to 3. Selected observations of isolated pulsars provide rather precise values of $n$, individually accurate to a few percent or better, in the range 1$ <$ n $ < $ 2.8, which is consistently less than the predictions of the MDR model. In spite of an extensive investigation of various modifications of the MDR model, no satisfactory explanation of observation has been found yet. The aim of this work is t...

  4. Nuclear Activity In Isolated Galaxies

    CERN Document Server

    Hernandez-Ibarra, Francisco; Krongold, Yair; Del Olmo, Ascencion; Perea, Jaime; Gonzalez, Jesus

    2012-01-01

    We present a spectroscopic study of the incidence of AGN nuclear activity in two samples of isolated galaxies (Karachentseva, V.E. & Varela, J.). Our results show that the incidence of non-thermal nuclear activity is about 43% and 31% for galaxies with emission lines and for the total sample 40% and 27% respectively. For the first time we have a large number of bona-fide isolated galaxies (513 objects), with statistically significant number of all types. We find a clear relation between bulge mass and the incidence of nuclear activity in the sample with emission lines. This relation becomes flatter when we take into account the complete sample with no emission line galaxies. A large fration ($\\sim$70%) of elliptical galaxies or early type spirals have an active galactic nucleus and $\\sim$70% of them are LINERs. Only 3% of the AGN show the presence of broad lines (a not a single one can be classified as type 1 AGN). This is a remarkable result which is completely at odds with the unified model even if we c...

  5. Isolation of Enterobacter cowanii in tomatoes after gamma irradiation

    International Nuclear Information System (INIS)

    The tomato is one of the most consumed fruit in the world. Bacteria of the family Enterobacteriaceae are responsible for large outbreaks of gastroenteritis. Irradiation is a physical method which reduces waste by eliminating spoilage organisms in foods. The objective of this study was to identify and determine the resistance profile of micro-organisms of the family Enterobacteriaceae from irradiated tomatoes. Were used three batches each containing 80 tomatoes, and divided in control and irradiated. The samples were individually properly identified as the irradiation dose applied. The material was subjected to irradiation with gamma rays, for irradiating with a cobalt-60 source, using doses: 1.0, 1.5 and 2 kGy (6,060 kGy/h). For microbiological analysis tomatoes were cut out, and removing the shells to obtain samples weighing 25g. Each sample was transferred to an Erlenmeyer containing sterilized water, stirring the assembly mechanically. Aliquots of the wash waters were sown in differential and selective media. After reisolation, the colonies were subjected to Gram staining then performed biochemical tests for identification. The antibiotic susceptibility tests were performed according to CLSI (Clinical Laboratory Standard Institute). It was isolated three strains of Enterobacter cowanii in tomato samples irradiated with a dose of 1.0 kGy, without isolating the other doses. As for the resistance profile, the strains were resistant to Ampicillin identified. Gamma irradiation at a dose of 1.5 and 2 kGy was effective in tomatoes as well as the micro-organism isolated after irradiation showed no profile of multidrug resistance. (author)

  6. Progress in organic spintronics

    International Nuclear Information System (INIS)

    Recent progress in organic spintronics is given an informative overview, covering spin injection, detection, and transport in organic spin valve devices, and the magnetic field effect in organic semiconductors (OSCs). In particular, we focus on our own recent work in spin injection and the organic magnetic field effect (OMFE). (topical review - magnetism, magnetic materials, and interdisciplinary research)

  7. Intercomparison of analysis methods for seismically isolated nuclear structures. Part 1: Advanced test data and numerical methods. Working material

    International Nuclear Information System (INIS)

    The purpose of the meeting was to review proposed contributions from CRP participating organizations to discuss in detail the experimental data on seismic isolators, to review the numerical methods for the analysis of the seismic isolators, and to perform a first comparison of the calculation results. The aim of the CRP was to validate the reliable numerical methods used for both detailed evaluation of dynamic behaviour of isolation devices and isolated nuclear structures of different nuclear power plant types. The full maturity of seismic isolation for nuclear applications was stressed, as well as the excellent behaviour of isolated structures during the recent earthquakes in Japan and the USA. Participants from Italy, USA, Japan, Russian federation, Republic of Korea, United Kingdom, India and European Commission have presented overview papers on the present programs and their status of contribution to the CRP

  8. Deceased Organ Donation.

    Science.gov (United States)

    Israni, A K; Zaun, D; Bolch, C; Rosendale, J D; Snyder, J J; Kasiske, B L

    2016-01-01

    SRTR uses data collected by OPTN to calculate metrics such as donation/conversion rate, organ yield, and rate of organs recovered for transplant but not transplanted. In 2014, 9252 eligible deaths were reported by organ procurement organizations, a slight increase from 8944 in 2012, and the donation/conversation rate was 73.4 eligible donors per 100 eligible deaths, a slight increase from 71.3 in 2013. Some metrics show variation across organ procurement organizations, suggesting that sharing best practices could lead to gains in efficiency and organ retrieval. PMID:26755269

  9. STM Studies of Isolated Mn12-Ph Single Molecule Magnets

    OpenAIRE

    Reaves, Kelley; Kim, Kyongwan; Iwaya, Katsuya; Hitosugi, Taro; Zhao, Hanhua; Dunbar, Kim R.; Katzgraber, Helmut G.; Teizer, Winfried

    2012-01-01

    We study Mn12O12(C6H5COO)16(H2O)4 (Mn12-Ph) single-molecule magnets on highly ordered pyrolytic graphite (HOPG) using low temperature scanning tunneling microscopy (LT-STM) experiments. We report Mn12-Ph in isolation, resembling single molecules with metallic core atoms and organic outer ligands. The local tunneling current observed within the molecular structure shows a strong bias voltage dependency, which is distinct from that of the HOPG surface. Further, evidence of internal inhomogeneit...

  10. Isolation of antimicrobial peptides and proteins from tomato

    Czech Academy of Sciences Publication Activity Database

    Králová, M.; Šanda, Miloslav; Macková, M.; Macek, Tomáš

    Praha : Ústav organické chemie a biochemie AV ČR, v.v.i, 2011 - (Slaninová, J.; Borovičková, L.). s. 41-41 ISBN 978-80-86241-36-4. [Biologically Active Peptides /12./. 27.04.2011-29.04.2011, Praha] R&D Projects: GA ČR GD305/09/H008 Grant ostatní: GA ČR(CZ) GA522/09/1693 Institutional research plan: CEZ:AV0Z40550506 Keywords : antimicrobial peptides * isolation * tomato Subject RIV: CC - Organic Chemistry

  11. The committed organic consumer

    OpenAIRE

    Geen, Natalie; Firth, Chris

    2006-01-01

    Abstract - UK committed organic consumers are reported to be about 23 per cent of consumers and account for 84 per cent of organic sales. This paper compares the committed organic consumer who is also a member of the UK’s national charity for organic growing (HDRA), to committed organic consumers in the wider UK population. Broad parallels were found between these two groups of consumers although there were differences in purchasing drivers as environmental issues were relatively more impor...

  12. Isolation and characterization of type IV group B Streptococcus capsular polysaccharide.

    OpenAIRE

    Wessels, M R; Benedí, W J; Jennings, H J; Michon, F; DiFabio, J L; Kasper, D L

    1989-01-01

    An antigenically distinct serotype, type IV, has recently been added to the recognized serotypes of group B streptococci (GBS). We isolated and purified the capsular polysaccharide antigen from a prototype type IV GBS strain. The type IV capsular polysaccharide formed a precipitin line with rabbit antiserum to type IV GBS organisms but not with antiserum to organisms of GBS serotype Ia, Ib, II, or III. Enzyme-linked immunosorbent assay inhibition experiments showed no cross-reaction between t...

  13. On Structure and Organization: An Organizing Principle

    OpenAIRE

    Baas, Nils A.

    2012-01-01

    We discuss the nature of structure and organization, and the process of making new Things. Hyperstructures are introduced as binding and organizing principles, and we show how they can transfer from one situation to another. A guiding example is the hyperstructure of higher order Brunnian rings and similarly structured many-body systems.

  14. Effectiveness of Base Isolation Technique and Influence of Isolator Characteristics on Response of a Base Isolated Building

    OpenAIRE

    Sunita Tolani; Dr. Ajay Sharma

    2016-01-01

    This study concerns with the seismic response comparison of a fixed base building with a base isolated building and parametric study of a base isolated building. The structural system considered for analysis is a three storey reinforced concrete building, which is idealized as a shear type building with one lateral degree of freedom at each floor level. The isolation systems considered for this study are Laminated Rubber bearing (LRB), Lead Rubber Bearing (N-Z bearing) and Frictio...

  15. Antifouling potential of some marine organisms from India against species of Bacillus and Pseudomonas

    Digital Repository Service at National Institute of Oceanography (India)

    Bhosale, S.H.; Nagle, V.L.; Jagtap, T.G.

    Crude methanolic extracts of 37 marine organisms (16 species of flora, 21 species of fauna) were screened for antibacterial properties against 5 strains of bacteria isolated from marine environments. Of these, 10 plant and 9 animal extracts...

  16. Prevalence and antimicrobial susceptibility of thermophilic Campylobacter in organic and conventional broiler flocks

    DEFF Research Database (Denmark)

    Heuer, Ole Eske; Pedersen, Karl; Andersen, J.S.;

    2001-01-01

    and sixty broiler flocks originating from organic, conventional and extensive indoor production farms were investigated for the presence of Campylobacter at the time of slaughter. Campylobacter isolates from a subsample of positive flocks were subjected to susceptibility testing. Campylobacter spp....... were isolated from 100% of organic broiler flocks, from 36.7% of conventional broiler flocks and from 49.2% of extensive indoor broiler flocks. Six of 62 Campylobacter isolates were resistant to one or more of the antimicrobials tested. Conclusions: These results indicate that the special...... characteristics of organic broiler production provide a high prevalence of Campylobacter positive flocks. Antimicrobial resistance was scarce among Campylobacter isolates from all rearing systems. Significance and Impact of the Study: Organic broiler flocks constitute a strong potential for introduction of...

  17. The complete genome of a viable archaeum isolated from 123-million-year-old rock salt.

    Science.gov (United States)

    Jaakkola, Salla T; Pfeiffer, Friedhelm; Ravantti, Janne J; Guo, Qinggong; Liu, Ying; Chen, Xiangdong; Ma, Hongling; Yang, Chunhe; Oksanen, Hanna M; Bamford, Dennis H

    2016-02-01

    Live microbes have been isolated from rock salt up to Permian age. Only obligatory cellular functions can be performed in halite-buried cells. Consequently, their genomic sequences are likely to remain virtually unchanged. However, the available sequence information from these organisms is scarce and consists of mainly ribosomal 16S sequences. Here, live archaea were isolated from early Cretaceous (∼ 123 million years old) halite from the depth of 2000 m in Qianjiang Depression, Hubei Province, China. The sample was radiologically dated and subjected to rigorous surface sterilization before microbe isolation. The isolates represented a single novel species of Halobacterium, for which we suggest the name Halobacterium hubeiense, type strain Hbt. hubeiense JI20-1. The species was closely related to a Permian (225-280 million years old) isolate, Halobacterium noricense, originating from Alpine rock salt. This study is the first one to publish the complete genome of an organism originating from surface-sterilized ancient halite. In the future, genomic data from halite-buried microbes can become a key factor in understanding the mechanisms by which these organisms are able to survive in harsh conditions deep underground or possibly on other celestial bodies. PMID:26628271

  18. Isolation of chloroplastic phosphoglycerate kinase

    Energy Technology Data Exchange (ETDEWEB)

    Macioszek, J.; Anderson, L.E. (Univ. of Illinois, Chicago (USA)); Anderson, J.B. (Pennsylvania State Univ., University Park (USA))

    1990-09-01

    We report here a method for the isolation of high specific activity phosphoglycerate kinase (EC 2.7.2.3) from chloroplasts. The enzyme has been purified over 200-fold from pea (Pisum sativum L.) stromal extracts to apparent homogeneity with 23% recovery. Negative cooperativity is observed with the two enzyme phosphoglycerate kinase/glyceraldehyde-3-P dehydrogenase (EC 1.2.1.13) couple restored from the purified enzymes when NADPH is the reducing pyridine nucleotide, consistent with earlier results obtained with crude chloroplastic extracts. Michaelis Menten kinetics are observed when 3-phosphoglycerate is held constant and phosphoglycerate kinase is varied, which suggests that phosphoglycerate kinase-bound 1,3-bisphosphoglycerate may be the preferred substrate for glyceraldehyde-3-P dehydrogenase in the chloroplast.

  19. Multiple isolated cutaneous plexiform schwannomas

    Directory of Open Access Journals (Sweden)

    Enas A. S. Attia

    2011-01-01

    Full Text Available Plexiform schwannoma is a rare neurogenic tumor, arising from skin and subcutaneous tissue. The presence of multiple schwannomas suggests a possible association with neurofibromatosis type 2 (NF2. A 50-year old male patient presented with multiple papulo-nodular cutaneous lesions on both arms and forearms. Histopathological examination revealed a dermal multinodular pattern of well-circumscribed masses of closely packed cells, with peripheral myxoid tissue, well-encapsulated in a thin collagenous capsule. S-100 immunohistochemical staining was diffusely and strongly positive. Neuron-specific enolase was positive, confirming a neural tissue tumor. An audiogram and Magnetic Resonance Imaging (MRI of cerebro-pontine angle showed no detected abnormality, excluding acoustic neuroma. Thus, we present a case of multiple bilateral isolated cutaneous plexiform schwannomas, not associated with NF2. Multiple plexiform schwannomas is a very rare entity, distinct from neurofibromatosis (NF, and being confined to the dermis is even more rarely reported.

  20. Spectroscopy of Isolated Prebiotic Nucleobases

    Science.gov (United States)

    Svadlenak, Nathan; Callahan, Michael P.; Ligare, Marshall; Gulian, Lisa; Gengeliczki, Zsolt; Nachtigallova, Dana; Hobza, Pavel; deVries, Mattanjah

    2011-01-01

    We use multiphoton ionization and double resonance spectroscopy to study the excited state dynamics of biologically relevant molecules as well as prebiotic nucleobases, isolated in the gas phase. Molecules that are biologically relevant to life today tend to exhibit short excited state lifetimes compared to similar but non-biologically relevant analogs. The mechanism is internal conversion, which may help protect the biologically active molecules from UV damage. This process is governed by conical intersections that depend very strongly on molecular structure. Therefore we have studied purines and pyrimidines with systematic variations of structure, including substitutions, tautomeric forms, and cluster structures that represent different base pair binding motifs. These structural variations also include possible alternate base pairs that may shed light on prebiotic chemistry. With this in mind we have begun to probe the ultrafast dynamics of molecules that exhibit very short excited states and search for evidence of internal conversions.